LOCUS       DBNHKV010000001         7118 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1771025, whole genome shotgun sequence.
ACCESSION   DBNHKV010000001 DBNHKV010000000
VERSION     DBNHKV010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1771025"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..702)
                     /gene="pgsA"
                     /locus_tag="AC16PP_00005"
     CDS             complement(103..702)
                     /gene="pgsA"
                     /locus_tag="AC16PP_00005"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012315213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR6480031.1"
                     /translation="MNLPNLLTLGRILFVPVFVLVYVIPGERTYLLAATLFALAAFTD
                     WLDGYLARRLNQTTPFGAFLDPVADKLIVVTALVLLIGHHRTLWLTLPALVIVGREIV
                     ISALREWMAEMNRRGVVAVSWLGRVKTTFQMIAVVVLLANPPAFDRPWVLLDYGLLYV
                     AAAMTLASMALYLHAAWPTLKSGYGGPEGEPGEDPRDRS"
     gene            complement(739..2556)
                     /gene="uvrC"
                     /locus_tag="AC16PP_00010"
     CDS             complement(739..2556)
                     /gene="uvrC"
                     /locus_tag="AC16PP_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019396703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR6480032.1"
                     /translation="MTAFDSEAFLGTLTRKPGVYRMLDADGNVLYVGKARNLRSRVSS
                     YFRASGLATKTMALVGRIADIQITLTNSETEALLLEQSMIKEERPPYNIVLRDDKSYP
                     FIYLTEGEPFPRLTFHRGAKRRKGRYFGPFPSASAVRDSLNILQKLFQLRHCEDSFFK
                     NRSRPCLQDQIRRCSGPCVGLVDSQTYAEDVKLAVMFLEGRSNAVLEAFKENMQKAAE
                     AMDYEAAAKYRDQIAHLRKVQEQQYVHAAEGDVDVFALMEASGVCCVQALFVRAGRML
                     GQRTWFPKNELGLEPAEMLSAFLSQYFLAGAERDVPRTVITAERIVDQDLLADALSKR
                     SGRRVDVLSQVRGQRARWVRLGLDNAAHSLNAYLADKRNVYARLVALQDLLGLEDVPE
                     RLECFDISHTMGEATVASCVVFDRNGPLKSDYRRFNIEDVAAGDDYAAMEQALRRRYT
                     RLKQGEGKLPDVLVIDGGRGQLQRAKDVLDELQVDGVHLLAIAKGPSRKPGLEKLFAN
                     GAEVDYPEQGAAAHLLQHIRDEAHRFAITGHRQRRQRKRSRSELDDVAGVGPKRRREL
                     LTHFGGLSGVKGASVEEIAKVPGISRRLAEEIYGTLHAE"
     gene            complement(2593..3231)
                     /locus_tag="AC16PP_00015"
     CDS             complement(2593..3231)
                     /locus_tag="AC16PP_00015"
                     /inference="COORDINATES: protein motif:HMM:NF015146.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR6480033.1"
                     /translation="MRYDRLESCRICPRLAAHLDQVRQAHPGYRCLPVGATGSVRAKL
                     LMVGLAPGLHGANRTGRPFTGDASGTFLFAALARVGLASDADPLVARLVGARLTNAVK
                     CLPPQNRPNAAEMSACSRYLREEIDELWSPKVRSARCVLCLGRLAHRSVEIALGKRLP
                     AFLHGQLCEVKPGLWLADSYHPSRQNTNTRRLTPEMLDSVLENVKGVVGLTG"
     gene            complement(3221..3883)
                     /gene="uvrY"
                     /locus_tag="AC16PP_00020"
     CDS             complement(3221..3883)
                     /gene="uvrY"
                     /locus_tag="AC16PP_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HIR6480034.1"
                     /translation="MIRVMVVDDHKLFRLGLRRMLADVKGIHVIGEADCGEAAVRLAR
                     AERPNIVLMDILMPGIGGIEATRRIARLEMDIKVIAVTGCHENPFPMQMLRAGACGYL
                     SKGVDRDELVAAIRKVFLGRRYLSADIAQEVAASAFDQTRESPFEQLSNREMQIMMMV
                     VNCHKVNDISEDLHLSPKTVNSYRYRIFEKLNVSSDVELALMAVRHGMVIPQLNGMAR
                     AV"
     gene            complement(4023..5201)
                     /locus_tag="AC16PP_00025"
     CDS             complement(4023..5201)
                     /locus_tag="AC16PP_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008367295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR6480035.1"
                     /translation="MPALEPFVWDDPLLLEDQLTEDERMVRDTAREYAQRELMPRVLK
                     ANREEHFDREIMNEMGALGLLGATLPAKYGCSAVNYVSYGLMAREVERVDSGYRSAMS
                     VQSSLVMHPIYAYGNEDQRERYLPKLASGEWVGCFGLTEPDHGSDPGGMVTRAEPVSG
                     GYRLNGAKMWITNSPIADLAVVWAKLDGVIRGFVVERAFDGFSTPKIEGKMSLRASVT
                     GEIVLDDVVVPEENLLPNVKGLAGPFGCLNRARYGIAWGSMGAAEFCWHAARTYTLDR
                     EQFGRPLAANQLIQKKLADMQTEIALGLQGCLRMGRLLDEDRLAVENISMLKRNNCGK
                     ALEVARMARDMHGGNGISDEYHVIRHVMNLETVNTYEGTHDIHALILGRAQTGLQAFS
                     "
     gene            complement(5284..7107)
                     /locus_tag="AC16PP_00030"
     CDS             complement(5284..7107)
                     /locus_tag="AC16PP_00030"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6480036.1"
                     /translation="MTPEAVIDKYLEVAGNAPVDRRVFLRLLGADADLLGRWLGLLRC
                     PVDVPALSEALADLDETTFVRLASSQAWAGLPVSGSARLSMDQWRSVLRGALLGESLA
                     EHLGIGDPESTRWRLLLAASGVNLRHDPRMSELLAFRGARAELLEDAGPEFRLYAVVD
                     ALEVADEEAAADLARGLLDLEASEFGELLEKADASLEALLAELGLAGELDADWAERVW
                     IRQQVNLLGLLFADCESLGAVQVAHHLASQSLFGYEPRLLVVNPAQETLEPVDGNGMS
                     IALASQTSSVAASVREGHPRTLIESPELAVADRQLLWRMDVAEGACRPLRQQEDLLGA
                     LIFSLDEDLDTEFAMSVYAEELTRWIVRASEHVEPAQGTLARYREREEKRLRELVHEA
                     NNPLSVVYNYLHILELRLQHEPSAVEQLRMIGEELKRTGDIIRRARELPPVEEPELSG
                     TVEISAVDVNRLVGQVFELHRGYAADRGVTLELEAAPGALVAASDESRLLQVLTNLVR
                     NAIEAAPDGSVTIGSLGGVFRDGVEGVELSVADSGPGLPREVLERLGDPKQSAKGGDH
                     AGLGLHIVHRLVEEVKARIDVRTSTGSGTTFTVFLPLSQPV"
BASE COUNT         1300 a         2380 c         2194 g         1244 t
ORIGIN      
        1 agtccgctgc atgaccactc tgccactcag ccttcgtcgc cggccggcgc gcgacgtcag
       61 tcgcggtcgc cctgcgaaca cgccggcgaa ttctagcggg ctttaggagc gatcgcgagg
      121 gtcttcgccc ggctcgcctt ccgggccgcc gtaaccggac ttcagcgtcg gccaggcagc
      181 atgcagatac agggccatgg aggcgagggt catggcggcg gcaacgtaaa ggagcccata
      241 gtcgagcagc acccagggcc gatcgaaagc cggcgggttg gccagcagca cgaccaccgc
      301 gatcatctga aaggtggttt tcaccctccc cagccaggaa accgcaacga cgccgcggcg
      361 gttcatttcc gccatccact cgcgcagcgc gctgatgacg atctctcgcc cgacgatcac
      421 cagcgccggg agcgtcaacc acagcgtgcg gtggtggccg atgagaagca cgagagcggt
      481 gaccacgatg agcttgtccg ccaccgggtc gagaaacgcc ccgaagggcg tggtctggtt
      541 gaggcgtcgg gccaggtaac cgtccagcca gtcggtgaat gcggccagcg caaacagcgt
      601 ggctgccagg aggtaagtac gttctcccgg gatgacgtag acgagcacga aaaccggcac
      661 gaaaagaatt cgccccagcg tgagcagatt gggtaagttg atgaccggtt tcatggctgt
      721 gtgctcaccg cgtaacgatc actccgcgtg cagggtaccg tagatctctt ccgccagcct
      781 gcggctgatt ccgggcacct tggcgatctc ctcgacgctg gcacctttca cgccggagag
      841 cccaccgaag tgggtcaaca gctcgcgccg ccgcttaggc cccacgccgg cgacgtcatc
      901 gagctccgat cggctgcgtt tgcgctgacg gcgttggcgg tggccggtga tggcaaagcg
      961 gtgcgcttcg tcgcgaatgt gctgaagcag atgagcggca gccccctgct ctgggtagtc
     1021 cacctccgcg ccgttagcaa acagcttttc cagtcccggc tttctgctgg gccctttggc
     1081 gatggccagc aggtgcacgc cgtcgacctg caactcgtcg agaacgtcct tagcccgctg
     1141 cagttgcccg cggccaccgt cgatcaccag cacgtcgggc agctttcctt cgccctgctt
     1201 caagcgagtg tatcgacgcc ggagcgcttg ctccatagct gcgtagtcat cgccggccgc
     1261 cacgtcctcg atgttgaatc gccgatagtc cgacttgagc ggcccgttgc ggtcgaagac
     1321 gacgcaggac gccaccgtgg cctcgcccat ggtgtggctg atgtcgaagc actccaggcg
     1381 ttccggcacg tcctccaggc ccagcagatc ctgaagagca accagccgcg catagacgtt
     1441 gcgcttgtcg gccagatagg cgttcaggct gtgggccgcg ttgtcgagac ccagcctcac
     1501 ccagcgggcg cgctggccgc gaacctggga gaggacgtcc acccggcgcc cggaccgctt
     1561 ggaaagcgcg tcggcgagaa gatcctggtc gacgatgcgc tccgcggtga tcaccgtgcg
     1621 cggcacgtcc cgttcagcgc cggccagaaa gtactgagac agaaaagcgc tcagcatctc
     1681 ggccggctcc agaccgagct cgttctttgg aaaccacgtc ctctgcccca gcatgcgccc
     1741 cgcgcgcacg aacagcgcct gcacgcagca cacgccggaa gcctccatca gggcgaagac
     1801 gtccacgtct ccttccgcgg cgtgaacgta ctgctgctcc tgaaccttgc gcaggtgggc
     1861 gatctgatcc cggtactttg ccgcggcttc gtagtccatt gcttccgccg ctttctgcat
     1921 gttctccttg aaggcctcta gcaccgcgtt gcttcgaccc tcgagaaaca tcaccgcaag
     1981 cttcacgtct tcagcatacg tctgggaatc gaccaggccc acgcagggac cgctgcagcg
     2041 tcggatctgg tcctgcaggc acggtcgcga ccggttcttg aagaacgaat cctcgcaatg
     2101 acgcagctga aagagcttct gcagaatgtt caggctgtcc cgcacggcgc tcgcggaagg
     2161 aaacggcccg aagtagcgac ctttgcgtcg ttttgcgccc cggtgaaacg tcagccgagg
     2221 gaagggctca ccttcggtga gataaatgaa cgggtaagat ttgtcgtcac gaaggacgat
     2281 gttgtacggc ggccgctcct ccttgatcat gctctgctcg agcaacagcg cttccgtctc
     2341 gctgttggtg agcgtgatct ggatgtccgc gatgcggccg accagagcca tggtcttggt
     2401 cgcgagtccc gaagcacgga aatagctgct gacccggctc ctgagattcc gggccttgcc
     2461 cacgtacaga acgtttccgt cggcgtcgag catgcgatag acgccgggtt tgcgcgtcag
     2521 ggtcccaagg aaagcttcgg agtcgaaggc cgtcatcggc ggaactatag agtccgcgcc
     2581 catgggccgc aatcagccgg tcaacccgac gacgcctttc acgttttcca ggaccgaatc
     2641 gagcatctcg ggtgtgagcc gacgcgtgtt cgtgttctga cggcttggat gataggagtc
     2701 ggccagccac aggcccggct tcacctcgca gagctgccca tgaaggaatg cgggcagacg
     2761 ctttccgaga gcgatctcca ccgagcgatg ggccaggcgc ccaaggcaca gcacgcagcg
     2821 cgccgatcgc accttcggag accacagctc gtcgatctcc tcgcgcaggt agcggctaca
     2881 ggctgacatc tccgcggcat tgggccggtt ctgcggcggc aggcatttga cggcgttcgt
     2941 caaccgggcc ccgacgagac gagcaacgag aggatcagcg tcactggcca atccgaccct
     3001 ggcaagcgcc gcgaaaagaa acgtgccgct ggcgtcccct gtaaaggggc gtcccgtgcg
     3061 gttggcgccg tgcagccccg gcgcgagccc gaccatcagc agcttggcgc gcacagaccc
     3121 cgtggcgccc acgggcaggc agcggtaacc gggatgggct tgccgtacct gatcgagatg
     3181 agcggccaac cgagggcaga tgcggcaact ttccaggcgg tcataccgca cgggccatcc
     3241 cgttcagctg gggtatcacc atgccgtggc gtaccgccat cagggcgagc tccacgtcgc
     3301 tggagacgtt gagcttctcg aagattcggt accgatagct gttcaccgtc ttcggcgaaa
     3361 ggtgcaggtc ctcggagatg tcgttcacct tgtgacagtt gaccaccatc atcatgatct
     3421 gcatttcacg attggaaagt tgctcgaacg ggctctcccg ggtctggtcg aacgcgcttg
     3481 ccgccacctc ctgagcaatg tcggcgctca gatagcgcct gccgagaaag acctttcgga
     3541 tggccgcgac cagctcgtcg cggtcgacgc ccttgctgag atacccgcag gctccggccc
     3601 ggagcatctg catggggaaa gggttttcgt ggcacccggt cacggcgatc accttgatgt
     3661 ccatctccag acgcgcgatc cgccgggtgg cttcgatgcc gccgataccg ggcataagga
     3721 tgtccatgag aacgatgttc ggtctctccg cgcgggcgag ccgcaccgcc gcctccccgc
     3781 agtccgcttc gccgattacg tgtatacctt tgacgtccgc cagcatgcgc cgcagcccca
     3841 gacgaaacag cttgtgatcg tccacaacca taaccctgat catcttgccc ccaacaaagt
     3901 gtccagcatc tgaagcagtg acgataggcg ggcgccaggc ggctaacaac ggcgcggggt
     3961 gagaatctgc cgggttttca cgaaatttcc attttccgat gtcgcgctga tatcagatac
     4021 gatcaggaaa acgcctgcag accggtctgg gcgcgcccca gtatcagcgc gtggatgtcg
     4081 tgggtcccct cgtaggtgtt gaccgtctcc agattcatga catgccggat cacgtggtac
     4141 tcgtcggaga tcccgttgcc gccgtgcatg tcccgcgcca tgcgcgcgac ctccagcgct
     4201 ttgccgcagt tgttgcgctt gagcatggag atgttctcga cggcaagacg atcctcgtcc
     4261 agcaggcgac ccatcctgag gcagccctgc aggccgaggg cgatctccgt ctgcatgtcc
     4321 gcgagcttct tctgaatcag ctggttggcg gcgagcgggc gcccgaactg ctcccgatcg
     4381 agcgtgtagg tgcgtgccgc atgccagcag aattcggctg cccccatgga tccccaggcg
     4441 atgccgtagc gcgcgcggtt caagcaaccg aagggacccg ccaggccttt gacgttgggc
     4501 agcagattct cttcgggaac aacgacgtcg tcgagcacga tctccccggt gaccgacgcc
     4561 cgcaggctca tctttccctc tatcttgggg gtgctgaaac cgtcgaacgc gcgctcaacc
     4621 acaaacccgc ggatcacgcc gtcgagcttt gcccagacca ccgccagatc ggctattggc
     4681 gagttggtga tccacatctt cgcaccgttc agacggtagc cgcccgacac cggctcggcc
     4741 ctggtcacca tgccgccggg gtccgatccg tggtcgggct ccgtcaggcc gaaacagccg
     4801 acccactcac cgctggccag cttcggcagg tagcgctcac gctgatcctc gttgccgtaa
     4861 gcgtatatcg gatgcatgac cagcgaggat tgcacgctca tggccgaccg gtaccccgaa
     4921 tccacgcgct ccacctcccg ggccatcagg ccgtaagaga cgtagttgac ggccgaacag
     4981 ccatacttcg ccggcagcgt ggcacccagc agaccgagcg cccccatctc gttcatgatt
     5041 tcccggtcga agtgttcttc gcgattggcc ttgagaacgc gcggcatgag ctcgcgctgc
     5101 gcgtattcac gcgccgtatc gcggaccatg cgctcgtctt cggtcagctg atcttccagg
     5161 agcaacggat cgtcccagac gaacggttcg agagcaggca tgggctcatc ccagtttgcg
     5221 gaaagtcagg cccaagggta ggagcgatac ggccgctagg caaccggcgc ctggccaggc
     5281 atatcagacc ggctgggaga gcggaaggaa gacggtgaaa gtggttccgc tgcccgtact
     5341 ggtacgaacg tcgattcgag ctttcacctc ctccaccagc cggtgaacga tgtgaagacc
     5401 gagacccgca tgatcgcctc ccttggcaga ctgcttcggg tcgccgagac gctccaggac
     5461 ttcccgcgga agtcccggac cgctgtccgc cacggaaagc tcgacaccct cgacaccgtc
     5521 gcgaaagacg ccgccgaggc tgccaatggt gacactgccg tcaggtgcag cctcgatggc
     5581 gtttcgaacc aggttcgtga gcacctgcag cagccggctt tcgtccgagg ccgccaccag
     5641 cgcgcccggc gccgcctcca gctcgagcgt caccccgcga tcggcggcgt aaccgcggtg
     5701 cagctcgaag acctgcccga cgaggcgatt cacatccacc gcgctgatct ccacggtgcc
     5761 cgacagctcg ggctcctcca cgggcgggag ctcgcgcgcg cggcgaatga tgtcgcccgt
     5821 ccttttgagc tcctcgccga tcatgcgcag ctgctctacc gcgctcggct cgtgctgcag
     5881 cctgagctcc aggatgtgga gatagttgta gaccacgctc agcgggttgt tggcctcgtg
     5941 aacgagttcc cgcagacgtt tttcttcccg ttcccgatag cgcgcgagcg tgccttgcgc
     6001 cggttcaacg tgttccgacg cccgaacgat ccatcgggtc agctcctcgg catagaccga
     6061 catggcgaac tcggtatcca gatcttcgtc cagcgagaag atgagggcgc caagcaaatc
     6121 ctcttgctgc cgaagcgggc ggcacgcgcc ctcagccacg tccatgcgcc acagaagctg
     6181 tcggtccgcg acggcgagct cgggagattc gatgagcgtg cgcggatgcc cctcccgcac
     6241 gctggcggca accgagctgg tttgcgatgc cagcgcgatg gacatgccgt tgccatcgac
     6301 cggctccagc gtctcctgcg ccgggttcac gaccagcagg cgtggctcgt agccgaaaag
     6361 actctgggag gctaggtgat gggcgacctg cacggcccct aaggactcgc aatcggcgaa
     6421 cagcaatcca agcagattga cctgctggcg gatccagaca cgctcagccc agtcggcatc
     6481 cagctccccc gccaggccca gctctgcaag cagagcctcg agggacgcgt ccgccttttc
     6541 cagaagctcg ccgaactcgg aggcctccag atcgaggagc cctcgtgcca gatcggccgc
     6601 cgcctcctca tcggcgacct ccagggcgtc caccacggca tagagcctga actccggacc
     6661 cgcgtcctcg agcagctccg cccgggcgcc tcgaaaggca aggagctctg acattcgtgg
     6721 gtcgtgacga agattgacgc ccgatgcggc cagcaggagc cgccacctcg tcgattcggg
     6781 atcgccgatt cccaggtgtt cggccagcga ctcgccaaga agggcgccgc gcagcacgct
     6841 gcgccattga tccatgctca accgcgcgct gccgctcacc ggaagtccag cccaggcctg
     6901 ggaggaggcc agacgcacga acgtggtttc gtcgaggtcg gcaagggcct cgctaagagc
     6961 cggcacgtcc accgggcaac gcagcagccc gagccagcga cccagcaggt ccgcgtcggc
     7021 gcccagaagg cgcaggaaca ccctcctgtc taccggcgcg ttgccggcaa cctcgaggta
     7081 tttgtcgatt accgcttcag gggtcaaccc ctacttct
//
LOCUS       DBNHKV010000002         4484 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1700230, whole genome shotgun sequence.
ACCESSION   DBNHKV010000002 DBNHKV010000000
VERSION     DBNHKV010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1700230"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..844)
                     /locus_tag="AC16PP_00035"
     CDS             complement(188..844)
                     /locus_tag="AC16PP_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HIR6480037.1"
                     /translation="MANWKTALVAGLVALGSAGCETAYYGAMEQVGVYKRDILVDRVE
                     DAVEAQQDAKEEFADALEQFASVVAVEPSRLKDTYEELSDAYDSAEDRAERVRDRIDS
                     VEDVSEDLFDEWSEEIELIGNPKLKSASASQLRASQRKYDELIKGMRRAERKMEPVLD
                     TFRGHVLFLKHNLNARAIASLKSELTSIEADVAGLIREMEASIAQSQAFIEEMQLVDG
                     "
     gene            1086..1895
                     /locus_tag="AC16PP_00040"
     CDS             1086..1895
                     /locus_tag="AC16PP_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6480038.1"
                     /translation="MTAYTDTAIELLMTYGPKFLLALVVLIIGLWIIKRVVKVTSKAM
                     ERGNAEPTLAKFVASLVSVGLKALLIISVASMIGIETTSFIAILGAAGLAVGLALQGT
                     LANFAGGALILLFRPYKVGDWIDAQGVAGTVHAIQIFNTILKTPDNRTIIIPNGAISN
                     GIITNNSTEPTRRVDFVFGIGYGDDIAKAKGIIERLIGADERTLSDPAAQIVVSALAD
                     SSVNLTVRVWVNAADYWGVFFDLTEKVKLAFDEEGVSIPFPQQDVHMHQAG"
     gene            complement(2138..3424)
                     /locus_tag="AC16PP_00045"
     CDS             complement(2138..3424)
                     /locus_tag="AC16PP_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6480039.1"
                     /translation="MAVPEGRRGLWGPASLLARTNLILAVGSLVIALIAIAALNVFVI
                     EPIAERSADDEAALLVLSAQTWVELPPAARPYFEIELAQNHDLIVSAEVRDLPFADLD
                     QPYLSLVEEKLSQRLNTPARLREGDDLIWVTVPMGGYDIQIGFASNRRDIQPLYVGII
                     IVGLGAAVVFITSLLIVLRIARPLVETAERAETYRGGENFEPLPERGPRELVSLTRNF
                     NTMAREITALLANRTTLLAGISHDLRTPLTRMRLALELLPDNVDPKLVERFERNLESM
                     DELITDALRFARGTSEVAQEVELKPFVEDVVASFDQSIPLTSDGDDVRVSVAPGALQR
                     VLINLITNALQHGKGVRVHIAGREVHVLDSGPGIPEEYRERVFQPFFRLDDSRSVATG
                     GSGLGLAIVQQLCQAQGWRVAIQEADNGEIDVFVTL"
     gene            complement(3411..4121)
                     /locus_tag="AC16PP_00050"
     CDS             complement(3411..4121)
                     /locus_tag="AC16PP_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6480040.1"
                     /translation="MDAEGAKLLVVDDDPEIRELAQAYLSQQGFEVDCVDSGEAMDAY
                     LESNEVDLIILDLMLPGEHGLSIARRLKTDGDVPIIIVSAQGEEIDRIVGLEVGADDY
                     IGKPFNPRELLARVRAVLRRAQRTGQASSAENRVCFGSFELDLSAHQLTADGKTVPLT
                     SGEFDLLAILVSHPNKVLDRDRILDLLTGAERSPFDRSIDVRVTRLRAKIEPDPSTPV
                     FIKTIWGKGYMFCPDGRA"
     gene            complement(4131..>4484)
                     /locus_tag="AC16PP_00055"
     CDS             complement(4131..>4484)
                     /locus_tag="AC16PP_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480041.1"
                     /translation="SIPNVYAAKSDQELTALAAQWESLDTAERRALLTEVKMRMARQR
                     GPDGTIRITFQRRYGRIIRNPDGSVVRIETNVVRVRPGAAGNQTYGVGFEQRTADGQR
                     EEQSPPVITVKDPSP"
BASE COUNT          835 a         1427 c         1363 g          859 t
ORIGIN      
        1 gcgtcgtccg ccgcggcgag gcccaccgcc tgatgcagct cgtctagggt ggccagggag
       61 atcgcgttgt gcttgtccgc ccggttgatg gtgatgagcc ccatggggcc ctggacttcg
      121 tagaggatgt tctcgaattc gctcacgtgc ctggttccgt tgtttggccc atcgtctgct
      181 gcgcgggtca gccgtcgacc agctgcatct cttcgatgaa ggcctgggat tgcgcaatgg
      241 acgcttccat ctcgcggatg aggcccgcca cgtccgcttc tatggaggtt agctcgcttt
      301 tcagggacgc gatggcccgg gcgttgagat tgtgcttcaa aaacagaacg tggccccgga
      361 aagtgtccag caccggctcc attttccgtt ccgcccgccg cattcccttg atcagctcgt
      421 cgtactttcg ctgtgaggct cgcagctggc tggcgctggc gcttttgagc ttcgggttgc
      481 cgatcagctc gatctcctca ctccactcgt cgaaaagatc ttccgagacg tcttcgaccg
      541 aatcgatacg gtcccgaacc cgctcggctc gatcctccgc cgaatcgtag gcatccgaca
      601 gttcctcgta ggtgtccttt agtctcgatg gctctacagc aaccacgctg gcgaactgct
      661 ctaaagcgtc tgcgaactcc tccttggcgt cttgctgggc ttccacggcg tcctcgacgc
      721 gatccacgag tatgtcgcgc ttgtaaacgc caacctgctc catcgcaccg taataggccg
      781 tttcgcagcc tgctgacccc agcgctacga gcccggctac aagcgccgtt ttccaattcg
      841 ccaccagcct tctcccaacc cgatacaaac cctttccacg acccgaccaa tctcaacgaa
      901 ctcgggaaca cggtgctgaa cgctcaggtc gggctgttgt ttctcgcgcc gatccttagg
      961 acaatcgcgt cccaaatcag gcgctcaacg cagaagcgcg cgcatagcgt gcccgagccc
     1021 catcaaaaca acaaccactg aaacacggga ggccacaccc catggaggag atttccacag
     1081 agcagatgac tgcttacacc gacaccgcca tcgagctgct gatgacctac ggacccaagt
     1141 tcctgttggc gctcgtcgtt ctcatcatcg gcctgtggat catcaagcgc gtcgtcaaag
     1201 tcacgtcaaa ggcgatggag cgtgggaacg ccgagcccac ccttgcgaag tttgtcgcaa
     1261 gcctcgtctc cgtaggcttg aaagccctgt tgatcatcag cgtggcatcg atgatcggca
     1321 tagagaccac gtccttcatc gccattctcg gtgccgccgg cctggccgtc ggtctggctc
     1381 tgcagggcac cctcgcgaat tttgcgggcg gcgctctgat cctgctcttt cgcccttaca
     1441 aggttggtga ctggatcgac gctcagggcg ttgcaggaac ggtgcacgcc attcagattt
     1501 tcaacacgat tctgaagaca ccggacaacc ggaccatcat catcccgaac ggcgccatat
     1561 cgaacggcat catcaccaac aactccaccg agccgacccg ccgcgtggac ttcgtgttcg
     1621 gcatcggcta cggggacgac attgccaaag ccaaaggcat catcgagagg ctgatcgggg
     1681 ccgacgagcg gaccctttcc gaccccgcgg ctcagatcgt ggtttccgcg ctcgccgaca
     1741 gctcggtcaa cctcaccgta agggtctggg tcaacgccgc cgattactgg ggcgtgttct
     1801 tcgatctcac cgagaaggtc aagctcgcat tcgacgagga gggagtttcc attccgttcc
     1861 ctcagcagga cgtgcacatg caccaggcag gctgacgctc gctggggtgt tttcgggcgc
     1921 cgcctgacca gcggcgctcg agcaaaaaaa aacgccggct tggagagaaa gccggcgtct
     1981 aaaggacctt ttacaaggtc tcaaggggat agagattgcc tcatggggaa gcggtttaac
     2041 ccgcttcgag gtccaatatg agggaaagac gtttcgccgc gatttcctgc cgcgaccttt
     2101 ttgtgacatt ttgtttcccg atgcccaccg gttcgaccta gagcgtaaca aagacgtcga
     2161 tctcgccgtt gtccgcttcc tgaatggcca ctcgccagcc ctgcgcctgg caaagctgct
     2221 ggacgatggc cagcccgagg ccgctgccac ccgtggctac actgcgtgaa tcgtcaaggc
     2281 gaaagaaagg ctgaaagacc cgctcccggt actcttcggg gatgcccggc ccgctgtcga
     2341 ggacgtgcac ctcccggccg gcaatgtgga cgcgcacgcc cttgccgtgc tgcaaggcgt
     2401 tggtgatcag gtttatcaac acacgctgca gcgcgcccgg ggcgacgctg acgcgaacgt
     2461 cgtctccgtc actggtcagc gggatggact ggtcgaagct ggcaaccaca tcctccacga
     2521 agggcttgag ctccacctcc tgcgccacct cggaagtccc ccgcgcaaat cgcagggcgt
     2581 cggtgatcag ctcgtccatg gattcgagat tgcgctcaaa tcgctcaacg agcttggggt
     2641 cgacattgtc cggcagcaat tcgagcgcca gccgcattcg cgtgaggggg gtgcgcaggt
     2701 cgtgggagat gccggcaagc agcgtcgtgc gattggcgag cagagcggtg atctcacgcg
     2761 ccatggtgtt gaaattgcgg gtcagcgaga ccagctcccg aggcccacgc tccggcagcg
     2821 gctcgaaatt ctcgccaccg cgataggtct cggcgcgttc ggcggtctcg accagagggc
     2881 gcgcgatcct gagcacgatg agcagcgacg tgatgaacac cacggcagca ccaagcccga
     2941 cgatgatgat cccgacgtaa aggggctgga tgtcgcgccg gttggacgcg aagcctatct
     3001 ggatgtcata cccgcccatg ggcacggtga cccagatgag gtcatcgccc tcacgcaggc
     3061 gagccggcgt gttcaggcgc tggctgagct tctcctcgac cagagaaagg tagggctggt
     3121 ccaggtctgc gaaaggcaga tcgcgaacct ctgcgctcac gatcagatcg tgattctgcg
     3181 ccagctcgat ctcgaagtac gggcgggctg ccggcggcaa ctctacccag gtctgggcag
     3241 aaagcacgag cagggccgct tcgtcgtccg ccgagcgctc cgcgatgggc tcgatgacga
     3301 aaacgttgag cgcggcgatg gcgatcagcg cgatgaccag cgaaccgact gccaggatga
     3361 ggttggtccg tgcgagcaga gacgccggac cccagagccc ccgccgcccc tcaggcacgg
     3421 ccatcggggc agaacatgta ccctttaccc cagatggtct tgatgaatac cggcgtggag
     3481 ggatccggct cgattttcgc ccgcagccgg gtcacccgca cgtcgatgct gcgatcgaag
     3541 ggcgaccgct cggctccggt cagcagatcc aggatgcggt ctcggtccag aaccttgttg
     3601 ggatgggaga cgaggatcgc gagcagatcg aactctcccg acgtgagcgg taccgtcttg
     3661 ccatctgccg tcagctgatg agccgaaagg tccagctcga agctcccgaa acacacgcgg
     3721 ttctctgcgg acgacgcctg cccggttcgc tgagcgcggc gcagcacggc tcgaacccgg
     3781 gcaagcagct cgcgggggtt gaacggcttg ccgatgtagt cgtctgcgcc aacctcgagc
     3841 ccgacgatgc ggtcgatctc ttcgccctgg gcagacacga ttatgatggg gacatcgccg
     3901 tcggtcttga gccggcgggc gatcgaaagg ccgtgctcac cgggcagcat gagatcgaga
     3961 atgatgagat ccacctcgtt ggattccagg taggcgtcca tggcctcgcc gctgtcgacg
     4021 cagtccacct cgaatccctg ctgggagaga taggcctgtg ccagctcacg aatctctggg
     4081 tcgtcgtcaa cgactagaag cttggcacct tcagcatcca cggcgggggc ttacggtgag
     4141 ggatccttga ccgtgatgac cggcggcgac tgctcctccc gctggccgtc tgcggtcctt
     4201 tgctcgaagc ctacgccgta ggtttgattg cccgccgcgc cggggcggac tcgaaccacg
     4261 ttggtctcga tccgaaccac ggacccgtcc ggattgcgga tgatgcggcc gtaacggcgc
     4321 tgaaacgtga tgcggatggt gccgtccgga ccgcgctggc gcgccatacg catcttgacc
     4381 tcggtcaaca gggcccggcg ctcggcggtg tccagggatt cccactgggc ggcgagagcc
     4441 gtcagctcct ggtcggattt ggctgcatag acgttcggga tgct
//
LOCUS       DBNHKV010000003         6389 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_410, whole genome shotgun sequence.
ACCESSION   DBNHKV010000003 DBNHKV010000000
VERSION     DBNHKV010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6389)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_410"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="AC16PP_00060"
     CDS             complement(<1..1026)
                     /locus_tag="AC16PP_00060"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6480042.1"
                     /translation="MNQLVRSPDLPDWPKQQLRGHRIDGYRYTSREFFEKEFEHMWAK
                     VWLLLGRESEIPNPGDWQREDVGPESILMVRQQDGGIKAFYNVCQHRGNRLVSEEKGH
                     VSRFVCKYHSWAFMPDGELIHAQDADDFPEGNPCGKVNLLEIPCESFAGFIWVNMDPE
                     CESLREYLGPIWDDWERYEIEKWKRYVALTTVAPCNWKVVLDNFNESYHVPTVHKPMG
                     TAAERKRMHSGVDTNYKHTRFDLSDQGHNRMIMPGGYAGATMNSEGVIGEPLASIMRE
                     WQLDPNDFINRGQETRTALQEAKRRLGPERGYTHYARMADEMLTDAFHYTLFPNFAVS
                     LWSDGFHF"
     gene            complement(1087..1875)
                     /locus_tag="AC16PP_00065"
     CDS             complement(1087..1875)
                     /locus_tag="AC16PP_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6480043.1"
                     /translation="MDLGIRGRKAIVNGGSAGMGRGSVLALAREGAELYVSARGAQRL
                     EEACEEIAGQTGASITPIVADHSTDEGREKILAACPEPDILVGTCAPPPVTGDYQAIT
                     ADDWRENLAVTLLSPVQFMQAVIPGMVERGWGRVVNIGTGAAKFPTQIRILSGAPRAA
                     LVNYTVAVAKAVAKHNVMINNILPGMHHTATIHERYTAAAAANGTTYDEEVQKFVDEW
                     GIPANKFGDPDDVGAFVALFCSEFANYTTGQSLVIDGGITNATF"
     gene            1970..3217
                     /locus_tag="AC16PP_00070"
     CDS             1970..3217
                     /locus_tag="AC16PP_00070"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6480044.1"
                     /translation="MATPAQYLDKLSGTALQRVARLADDIVTGGDEAQQLRRLPDWLV
                     DKLIDEGFFRFTLPPELGGEDASTLETIEVLEAISAMDASVGWNVMLGSEINAMAAGG
                     MPKELAKEVYIDNPSVVMCGGGGPGSQPSYAIEQDDGSYRVWGETTFISGCHNATWCF
                     MGAPIIDDGQPRTDGNGMPIIKMWFLHRSQWDIIDTWDVAGLRGSGSHNVRAEGALVE
                     PKWLPVELMVTPALYDNPVFRIPVPLRLSYNKVAIALGVARGALDAFADLANNKVPML
                     SVSKLKDRPIAKYRMAEATATYRAARAYVFDTMGAVEEELRKGADFPSTETTVNARLA
                     CIHAAQICKDLVDDVHTAAGTSAAYMKNPLERKLRDAHGAASHRWVAHPLYETIGGIL
                     FGEEPDAEFAGTGGPVLGGAGKK"
     gene            3265..4128
                     /locus_tag="AC16PP_00075"
     CDS             3265..4128
                     /locus_tag="AC16PP_00075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6480045.1"
                     /translation="MKIGTFASITTPAASPATILDMGRRAEAAGLDSLWMGEHVVLFD
                     EMEFGYPGSADGRLPIPHGSGLPDTVVTLSYLAGVTENLRLGTGVSLIPQRNPIYTAN
                     EFATLDYLTGGRVDLGVGVGWCKEEVLACGYEWETRGERCDEILELMIKLWTEPVTTH
                     HGKHFRVDDCRMDPKPVQQPHVPLIVGGYSPAAMRRAARYGAGWIGFGTNPEQTAMAL
                     KGIDAALEAAGRSRDGFEIVVMPAEANADTVRAFEDIGVDRLVPLLVASGPDAVARRI
                     DELAELKALLA"
     gene            complement(4153..4680)
                     /locus_tag="AC16PP_00080"
     CDS             complement(4153..4680)
                     /locus_tag="AC16PP_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6480046.1"
                     /translation="MVRETGQVPGLEEVADRLAILDVLAKHSRGVDRADAEVLKSAYW
                     PDAQVAYGGFNGLAHEFCDYLPGAIKRYARTQHSISNTAIEFSGDEARVETYVTAYHY
                     LEVDGDQGTEMTYLGRYMDRMQKRGDVWKIIHRQVVMDWNQNVDTTAVWEGPTFSGLA
                     RGARAPDDPVNALLG"
     gene            4759..5907
                     /locus_tag="AC16PP_00085"
     CDS             4759..5907
                     /locus_tag="AC16PP_00085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012336.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6480047.1"
                     /translation="MSTDVAIIGIGIHPFGRTEGVSGLDQGVFAARQALKDVRLDWTD
                     MQFAYGGSAAAGSADTMVNKLGLTGLQFINVANGCATGGSALQSAFMAIQSGMFDIGM
                     AVGFDKHPRGAFTVDPEKRGLGKWYGDIGMALTPQFFAMKIQRYMHDYGITEDALTKV
                     AVKNYKNGALNPNAWRRNAFSYEEVAQSPMVCDPLRKYMFCSPAEGGVAMILCRADKA
                     AEYTSKPVFLDAVSVKTRNFGSFEVYTQGQALDRADSPTVMASKAAFEMAGIGPEDID
                     VAQLQDTESGAEIMHMAENGFCADGDQEKWLAEGRTEIDGRLPVNTDGGCLANGEPVG
                     ASGLRQVYEVALQLRGEAGERQVPNTPRTGYTHVYGAPGLSGVTILSR"
     gene            complement(5921..>6389)
                     /locus_tag="AC16PP_00090"
     CDS             complement(5921..>6389)
                     /locus_tag="AC16PP_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6480048.1"
                     /translation="KHPRVLFADIQREALAKVIPSIADKIADIEFSSVFFTVFPNFHP
                     WGSFNRINYRFRPNGRNVDECIMECIYLAPIPENGEYTPCREIHWLGPDDDWTEAPEL
                     GMLAKVFNQDVRNLPYVQEGLHATAKEHLQFADYNETKPRHLHMLMDEWIAKP"
BASE COUNT         1119 a         2116 c         2068 g         1086 t
ORIGIN      
        1 gaagtgaaag ccgtcggacc acaaagacac cgcgaagttg gggaacagcg tgtagtggaa
       61 ggcgtcggtg agcatctcgt cggccatcct ggcgtagtgg gtgtagcccc gctcaggccc
      121 cagtcggcgc ttcgcctcct gcagcgccgt gcgggtctcc tggccccggt tgatgaagtc
      181 gttcggatcc agctgccact cgcgcatgat cgaggccagc ggctcaccga tcacaccctc
      241 gctgttcatg gtcgcgcccg cgtagccgcc gggcatgatc atgcggttgt ggccctggtc
      301 ggagaggtcg aagcgcgtgt gcttgtagtt ggtgtccacc ccggagtgca tgcgcttgcg
      361 ctccgctgca gtgcccatgg gcttgtgcac cgtgggcacg tggtaggact cgttgaagtt
      421 gtcgagcacc accttccagt tacacggcgc taccgtggtg agcgccacgt agcgcttcca
      481 cttctcgatc tcgtagcgct cccagtcgtc ccagatgggc ccgagatact cacgcaggct
      541 ttcgcactcc ggatccatgt tgacccagat gaagccggca aacgactcgc agggaatctc
      601 cagcaggttg actttgccgc aggggttgcc ttcggggaag tcgtccgcgt cctgggcatg
      661 gatcagctcg ccgtcgggca tgaacgccca gctgtggtac ttgcagacga agcggctcac
      721 atggcccttc tcctcggaga cgagccgatt gccgcggtgc tggcagacgt tgtagaaggc
      781 cttgatgccg ccgtcttgct ggcggaccat gaggatggac tcgggaccaa cgtcctcgcg
      841 ctgccagtcg cccggattcg gaatctccga ttctcgcccg agcagcagcc agaccttggc
      901 ccacatgtgc tcgaattctt tttcgaagaa ttcccgggac gtgtaacggt agccgtctat
      961 tctgtggccg cgaagctgct gcttcggcca atcaggcaga tcggggctgc gtaccagctg
     1021 attcaatcaa ccaccctcct caatccgcgg aatagtataa cgcgtttccc ttttggtcca
     1081 gcaggctcaa aacgttgcgt tggtgatgcc cccgtcgatc accaggctct ggcccgtggt
     1141 gtagttggcg aactcgctgc agaagagcgc aacgaaagcg cccacgtcat ccggatcgcc
     1201 gaacttgttc gccgggatcc cccactcgtc gacgaacttc tgcacttcct catcgtaggt
     1261 ggtgccgttc gcggccgcgg cggccgtgta acgctcgtgg atcgtcgcgg tgtggtgcat
     1321 ccccggcagg atgttgttga tcatgacgtt gtgcttggcc acggccttgg ccaccgccac
     1381 ggtgtagttg accagggcag cgcggggggc gcccgacaga atacggatct gggtgggaaa
     1441 cttcgccgct cccgtgccga tgttgaccac cctgccccaa ccccgttcca ccatcccggg
     1501 aatcacggcc tgcatgaact gcaccgggct gagcagggtg accgccagat tctcccgcca
     1561 gtcgtcggcg gtgatggcct ggtagtcgcc tgtcaccggc ggcggggcgc aggtgcccac
     1621 gagaatgtcg ggttcaggac acgccgccag gatcttctcg cgaccctcgt cggtgctgtg
     1681 atcggccacg atgggcgtga tgctggcgcc cgtctgcccc gcgatctcct cgcaggcttc
     1741 ctcgagccgc tgcgcacccc gcgccgagac gtagagctcc gccccttccc gcgccagcgc
     1801 cagaacgctg ccgcgcccca tgccggcgct gccgccgttg acgatggcct tgcggccgcg
     1861 aatgccgaga tccactgtat gccctcctcc catacgtaca ttccgcatcg tagcgcgaaa
     1921 agtaaacgcg ttatactgac tgctggagca cggcactgag ggaaacgcca tggcgacacc
     1981 cgctcaatat ctggacaaac tcagcggcac cgcgctgcaa cgggtcgctc ggctggccga
     2041 cgacatcgtc accggcggcg acgaggctca gcagctcagg cgcctgcccg actggctggt
     2101 ggacaagctc atcgacgagg gcttcttccg cttcacgctg cccccggagc tgggcggcga
     2161 ggacgcgagc acgctggaga ccatcgaggt tttagaggcg atttctgcca tggacgcttc
     2221 cgtcggctgg aacgtcatgc tcggatccga gatcaacgcc atggccgccg gcggcatgcc
     2281 gaaggaactc gccaaagagg tctacatcga caaccccagc gtcgtcatgt gcggcggcgg
     2341 tggcccggga tctcagcctt cctacgccat cgagcaggac gacggcagct atcgcgtgtg
     2401 gggtgaaacc acgttcatca gcggctgtca caacgccacc tggtgcttca tgggcgcgcc
     2461 catcatcgac gacggccagc cccgcacgga cggcaacggc atgcccatca tcaagatgtg
     2521 gtttctgcac cgctcccagt gggacatcat cgacacgtgg gacgtggccg gtctgcgcgg
     2581 ttcgggcagc cacaacgtgc gagccgaagg cgccctggtg gagccgaaat ggctgcccgt
     2641 ggagctcatg gtgacacccg ccctctacga caacccggtg ttcaggattc ccgtgccgct
     2701 gcggctgtcc tacaacaagg tggccatcgc cctgggcgtc gccagaggcg cgctcgacgc
     2761 cttcgcggac ctggccaaca acaaggtgcc aatgctgtcc gtatcgaagc tcaaggacag
     2821 gcccatcgcc aagtaccgca tggccgaggc caccgccacc tatcgcgcgg ccagggccta
     2881 cgtcttcgac accatgggcg cggtggagga agagctgaga aagggcgccg attttccctc
     2941 cacggaaacc acggtcaacg cgcgcctggc ctgcatccat gcagcccaga tctgcaagga
     3001 cctggtggac gacgtccaca ccgcggcggg aaccagcgcg gcgtacatga agaacccgct
     3061 ggaacgcaag ctgcgagacg cccacggggc cgcgtcccat cgctgggtcg cgcacccgct
     3121 ctacgagacc atcggcggca tcctcttcgg cgaggagccc gacgccgagt tcgcgggaac
     3181 gggcggcccc gtgctgggag gcgctggcaa gaagtgagcg ggcccggtca aggcccgcag
     3241 ccccgcaagg acgaggtaac aaacatgaag atcgggacgt tcgcctccat caccacgccg
     3301 gcagccagcc cggcgaccat tctcgacatg ggccggcgcg ccgaggccgc tggtctggat
     3361 tcattgtgga tgggcgagca cgtggtgctc ttcgacgaga tggagttcgg gtatcccggc
     3421 tcggctgacg ggcgcctgcc gatcccccac ggctccgggc tgccggacac ggtggtgacg
     3481 ctgagctacc tggccggcgt gacggaaaat ctcaggctcg gcaccggcgt ctcgctgatt
     3541 ccacagcgca acccgatcta caccgccaac gagttcgcca cgctggatta cctcacgggc
     3601 ggcagggtcg atctcggcgt tggcgtaggc tggtgcaagg aggaagtgct ggcctgcggc
     3661 tacgagtggg agacccgggg cgagcgctgc gacgagatcc tggagctgat gatcaagctg
     3721 tggaccgagc ccgtcaccac ccaccacggc aagcacttcc gcgtggacga ctgccgcatg
     3781 gacccgaagc ccgtgcagca gccgcacgtc ccgctcatcg tgggcggcta cagccccgcc
     3841 gccatgcgcc gagccgcgcg ctacggcgcc ggctggatcg gcttcggcac caaccctgag
     3901 cagacggcca tggcactcaa aggcatcgat gccgcgctgg aagccgccgg gcgctcccgg
     3961 gacggcttcg agatcgtcgt catgccggcc gaggccaatg cggacaccgt gcgcgcgttc
     4021 gaggacatcg gcgtggaccg gctggtcccg ctgctggtgg cgagcggtcc cgacgccgtg
     4081 gcgcggcgca tcgacgagct ggcggagctc aaggcgctgc tggcctgacc cggcggcgag
     4141 ccggccggga tgtcagccta ggagcgcgtt gaccgggtcg tccggggcac gggcgccccg
     4201 cgccagcccc gagaacgtcg gcccttccca gacggccgtc gtgtccacgt tctggttcca
     4261 gtccatgacc acctggcggt ggatgatttt ccagacgtca ccgcgcttct gcatgcggtc
     4321 catgtagcgg ccgagatagg tcatctcggt gccctggtcg ccgtcgacct ccaggtagtg
     4381 gtaggcggtc acgtaggttt ccacccgcgc ttcgtcgccc gagaactcga tggccgtgtt
     4441 gctgatgctg tgctgagtac gcgcgtagcg cttgatagcg cctggcaggt agtcgcagaa
     4501 ctcgtgggca agaccgttga accccccgta cgccacctgg gcatccggcc agtaggccga
     4561 cttcaggacc tcggcatccg cgcgatccac gccccggctg tgcttggcaa gaacgtccag
     4621 gatggcgagc cgatcggcca cctcctccaa accgggcacc tgccccgtct ctctgaccat
     4681 gatgcgatcc ctccctgacg accgagccca agacaatact gtagaatctg ctcatctgaa
     4741 accgggaggt gagcaggcat gtctacagac gtagcgatca tcggtatcgg catccaccct
     4801 tttggacgga cggagggcgt ctccggcctg gaccagggcg tgttcgccgc gcggcaggcg
     4861 ctcaaggacg tgaggctcga ttggaccgac atgcagttcg cctacggcgg aagcgccgcc
     4921 gccggctccg cagacacgat ggtcaacaag ctcgggctca ccgggctgca gttcatcaac
     4981 gtcgccaacg gctgcgccac cggcggcagc gctctgcaat ccgccttcat ggccatacag
     5041 tcgggcatgt tcgacatcgg catggccgtg ggtttcgaca agcacccgcg cggcgctttc
     5101 accgtggatc cggaaaagcg cggtctgggc aagtggtacg gggacatcgg catggcgctc
     5161 acgccgcagt tcttcgccat gaagattcag cgctacatgc acgactacgg catcaccgag
     5221 gacgcgctga ccaaggtggc ggtgaagaac tacaaaaacg gcgctctgaa ccccaacgcc
     5281 tggcgacgca acgccttcag ctacgaggag gtggcccagt cgcccatggt ctgcgacccg
     5341 ctgcgcaagt acatgttctg ctcaccggcc gagggtggcg tggccatgat cctgtgccgc
     5401 gcggacaagg cggccgagta caccagcaag ccggtctttc tcgacgcggt cagcgtgaag
     5461 actcgcaact tcggctcctt cgaggtttac acccagggcc aggcgctcga ccgggccgat
     5521 tcgcccacgg tgatggcgtc caaggccgcc ttcgagatgg ccggcatcgg cccggaggac
     5581 atagacgtcg cccagctgca ggacaccgaa tccggcgccg agatcatgca catggcggaa
     5641 aacggcttct gcgccgacgg cgatcaggag aagtggctgg ccgaagggcg gacggagatc
     5701 gacggcaggc tgccggtcaa cacagacggc ggctgcctgg ccaacggcga gcccgtgggc
     5761 gcgtcaggcc tgcgacaggt ctacgaggtg gcgctgcagc tcagaggaga ggccggtgag
     5821 cggcaggtgc ccaacacacc ccgcacgggt tacacccacg tgtacggcgc accgggcttg
     5881 agcggcgtca ccatcctgtc acgctgaagc taccgccggg tcagggcttc gcgatccact
     5941 cgtccatcag catgtgcagg tggcgcggct tggtctcgtt gtagtccgcg aactgcaggt
     6001 gctccttggc ggtggcgtgc agaccctcct gcacgtaggg gaggttgcgc acgtcctggt
     6061 tgaacacctt cgccagcatt cccagctccg gcgcctccgt ccagtcgtcg tcgggcccca
     6121 gccagtgaat ctccctgcac ggcgtgtact cgccgttctc gggaatcggt gccaggtaga
     6181 tgcactccat gatgcactcg tccacgttcc ggccgttcgg acggaaacgg tagttgatcc
     6241 ggttgaacga cccccagggg tgaaagttgg gaaagaccgt gaagaagacg gacgagaact
     6301 cgatgtccgc gatcttgtcc gcgatcgagg gaatcacctt ggccagcgcc tcgcgctgga
     6361 tgtcggcgaa cagaacccgc ggatgcttg
//
LOCUS       DBNHKV010000004         5872 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1062855, whole genome shotgun sequence.
ACCESSION   DBNHKV010000004 DBNHKV010000000
VERSION     DBNHKV010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5872)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1062855"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="AC16PP_00095"
     CDS             <1..396
                     /locus_tag="AC16PP_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480049.1"
                     /translation="QLGLSSNWPVLRWFRLAIARADIDATALIVHFDDQGGVRAYGLD
                     QRSIDLGGGTGFQPIFVVTPLVDADEYLEDIWPAHRWGMSMLREAPATLNAHAGVDSG
                     MQGIEMRSTSLKVGQRTLEMQKEQSSSSR"
     gene            complement(407..1612)
                     /locus_tag="AC16PP_00100"
     CDS             complement(407..1612)
                     /locus_tag="AC16PP_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR6480050.1"
                     /translation="MNARGLIVVASMLLVGSVGWAEPDEYAVDAEDDFELAAQEMADA
                     MADGGFELDDAKFAGNPMQRLFKKWPEDLVVAPIPSRSPQLGFQLSVVGGYFLGDRGE
                     DAKKRPSAIGGFAMASDNGSYAFGAGGKFHLLDDRLRLQAGAGYADIRYKFYGFGDFV
                     GDSGINIEIEQEAPLYFANAKWRFWNQLYAGIGFVRGNVETRTRVPLPPEWPREITPS
                     FNVDLGALWVPVQFDSRDHEQFPRRGWLIDGDTKIYRDDFGSDFEAETYKLALNHYRA
                     MRTRDALALRAVIKATGGNAPFFLLSSFGSGPDLRGYPAGRYRDKLMYAVQGEYRWHL
                     HDSWILTGFAGVGEVADEIGDFGDDFLPAAGIGARYVLSKKHRVSISADLAVGKHGAE
                     FYIGIGEAF"
     gene            complement(1614..3314)
                     /locus_tag="AC16PP_00105"
     CDS             complement(1614..3314)
                     /locus_tag="AC16PP_00105"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR6480051.1"
                     /translation="MVARRLRAAAALAALLLITGIATVEAASRPKLILQITVDQLRAD
                     LPTRYYERLGKGGFKYLWEKGIVFTNAHHAHANTETIVGHATLATGAHPSVHGMVGNL
                     WFDRETGFTTYNVEDGDHRLLTEAADVDDATEIDPTQRAARSEGRSPAAILVTTFADE
                     LRSNTNGRAKAIGVSVKDRGAISMAGHSGTAYWFSKATGGFVTSTYYLDRYPDWVSDF
                     NAAGPAAAYAGTAWELLREQDTYLFGEADDRPWETDVAGFGRVFPHAFGDPSSPYFAT
                     WLTLSPAGDRLVLDFAKRAIVEESLGQDATTDYLSVSFSSTDYVGHVFGPSSLEAEDN
                     ILRLDRTLAELFAFVAKQVGLADTLIVLSADHGAPDSPGYLNSLGIPGGYVDPDSWDK
                     ADAIDRIKARFGIEGALFEKYAHPYLYFSPEVAGNEQIDRGALEAAVIEELTKLPGVA
                     LAVSSTALGSASIPDSEIHRRVLRNHHPDRSGDVFIVFEPNWFINDFDGLTVASTHGS
                     PWRYDTHVPVVFAGARLRPAKIDRAVRTVDVAPTLSALLQIKAPSGSVGEPLVEVLRR
                     "
     gene            complement(3316..4884)
                     /locus_tag="AC16PP_00110"
     CDS             complement(3316..4884)
                     /locus_tag="AC16PP_00110"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007977712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR6480052.1"
                     /translation="MKSSRRTSTTSILLALLFASMSAWSQDKPNILVIWGDDVGWSNI
                     SAYNLGMMGYQTPNIDRIAEEGALFTHYYAHQSCTAGRSSFILGQHPFRTGLLTIGMP
                     GADHGIPDWSPTIADLLKNHGYATGQFGKNHLGDRDEHLPTNHGFDEFFGNLYHLNAE
                     EEPETYHYPKDPEFRARFGPRGVIKSTADGQIQDTGPMTRKRMETADEEFLGSAMDFI
                     DRSVKADKPFFVWMNTTRMHVWTRLKAEAEGVTEMGLYPDGMAEHDGHVGLLLDQLDR
                     LGIADNTIVIYSTDNGAEKVTWPDGGTTPFHGEKGTTWEGGMRVPAVVRWPGTIKPGT
                     KIHDIMSHEDWLPTLLAAAGENDVVQKLKKGHRANGKRWRVHPDGYNFLPYLKGEADA
                     PREEIFYFGQNGDLDAVRWRDWKVSFATFDGNIATGIREVANWPVITHLRSDPFEEMH
                     YESSMYLRWMADNIWLFVPIQAKVQEFLSTMGDYPFQEGQSLNAAGINYRSLKALQIL
                     QQLNQKGVLSPPTN"
     gene            5192..>5872
                     /locus_tag="AC16PP_00115"
     CDS             5192..>5872
                     /locus_tag="AC16PP_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR6480053.1"
                     /translation="MRHWPPSRTSPIGAADIIFKAMLVPAVLLAVAACTREAEPRWLL
                     RALPGEYPEIVYFVPTTSRAVAMTIDDGLDPETTPLILETLKAHGASATFFVLSDSIA
                     GREVLLQRILYEGHEIGHHMTEDEVTVSLKPAAMAEKFHRAAQTLDAFAPITWFRPGS
                     GRYNDAVLELTRARGYRIALASVAPLDTVIHNSARMAGFVERMIEPGSVVVLHDVGER
                     GRRTAETLK"
BASE COUNT         1026 a         1889 c         1883 g         1074 t
ORIGIN      
        1 cagctcgggc tctcgtccaa ctggcccgtg ttgcgctggt tccggctggc catcgcccga
       61 gccgacattg acgccaccgc gttgatcgtg cacttcgacg accagggtgg cgtccgggcc
      121 tatggcctgg atcagcgatc catcgatctg ggcgggggca cgggcttcca gccaattttc
      181 gtggtgacgc ccctggtgga cgcggacgag tatctcgagg acatctggcc cgcccaccgc
      241 tggggcatgt ccatgctgcg cgaagccccc gccacgctga acgcccatgc gggcgtggat
      301 tccggcatgc aggggattga aatgcgctcg acgtcgctga aggtggggca gcgcaccctg
      361 gagatgcaga aagagcaaag cagctccagc cgctgaacgg ccgcggtcag aacgcctcgc
      421 cgatgccgat gtagaactcg gcgccgtgct tgccgacggc caggtccgca gaaatactca
      481 cccggtgctt cttcgacagc acgtagcgcg caccaatgcc cgccgccggc aggaagtcgt
      541 cgccgaagtc gccgatctcg tcagccacct cacccacccc ggcaaagccc gtcaggatcc
      601 agctgtcgtg aaggtgccag cggtactcac cctgcacggc gtacatgagc ttgtcacggt
      661 agcgtccggc cgggtaaccg cgcagatcgg ggccgctgcc gaagctgctg agcagaaaga
      721 acggcgcgtt accaccggtg gccttgatca ccgcacgcag ggccagagcg tcccgggtgc
      781 gcatggcccg atagtgattc agagcgagct tgtaggtctc cgcttcgaaa tcgctgccaa
      841 agtcgtcgcg gtagatcttg gtgtccccat cgatcagcca tccccgtcgg ggaaactgct
      901 cgtggtcgcg gctgtcgaac tgcaccggca cccacagcgc gccgaggtcc acgttgaacg
      961 acggggtgat ctctcgtggc cactcgggag gcagaggaac ccgcgtcctc gtctcgacgt
     1021 tgccgcggac gaagccgatt cccgcgtaaa gctggttcca gaaacgccac ttggcgttgg
     1081 cgaagtaaag cggtgcttcc tgctcgatct cgatgtttat gcccgagtca ccgacgaagt
     1141 cgccgaagcc gtagaacttg taacggatat cggcgtagcc cgcgcccgcc tgcaggcgca
     1201 ggcggtcgtc cagcaagtga aatttcccgc cggccccgaa cgcgtagctg ccattgtccg
     1261 aggccatggc gaagcccccg atggcagacg ggcgcttttt tgcgtcttca cccctgtcgc
     1321 caagaaaata tcccccgacc acggacagct ggaagccgag ctgaggcgat cggctgggga
     1381 tgggcgcgac gaccagatcc tccggccact tcttgaacag gcgctgcatg gggttgccgg
     1441 cgaatttggc atcgtcgagc tcgaagcccc catcggccat ggcatccgcc atttcctgcg
     1501 cggccagctc gaaatcgtcc tccgcatcga cggcgtactc gtccggttcg gcccaaccaa
     1561 ccgagccaac aagcagcatg gaggccacca cgatgagtcc gcgcgcattc atcctatcgc
     1621 ctcaacacct cgaccagcgg ctcgccaacg gagccgctgg gcgccttgat ctgcagcagc
     1681 gcggacagcg tcggcgcgac atctaccgtg cgcacggcac gatcgatctt tgccgggcgg
     1741 agccgagcgc cggcaaacac gacgggcacg tgggtgtcgt agcgccaggg agaaccgtgg
     1801 gtggaggcca ccgtgagccc gtcgaagtcg ttgatgaacc agttgggctc gaacacgatg
     1861 aacacatcgc cggagcggtc cggatgatgg ttgcgcagaa cgcggcgatg gatctccgag
     1921 tcgggaatgc tggccgagcc cagggccgtg ctggacacgg ccagggcaac tccgggcaac
     1981 ttcgtcagct cctcgatcac cgccgcttcc agggcgcctc ggtcgatttg ctcgttgccg
     2041 gcgacttccg ggctgaagta gagatacgga tgagcgtact tctcgaacag cgcgccctcg
     2101 atcccgaatc gcgctttgat ccgatcgatg gcgtcagcct tgtcccagct gtccgggtcg
     2161 acgtagccac ccgggatacc caggctgttc aggtatccgg gagaatccgg cgcgccgtga
     2221 tcggcggaaa gcactatcag cgtgtcggcc aggcccacct gcttggcgac gaaggcgaac
     2281 agctccgcca gggttcggtc gagccggaga atgttgtctt ccgcctccag gctggacggt
     2341 ccgaagacgt ggccaacgta gtcggtggag gaaaagctca cgctcaggta gtcggtggtc
     2401 gcgtcctgcc ccagcgactc ctcgacgatg gctcgtttgg cgaagtcgag caccagccgg
     2461 tccccggccg ggctcagggt cagccaggtg gcgaagtagg ggctgctggg gtcgccaaag
     2521 gcgtggggaa atacccggcc gaatcccgcc acgtcggttt cccagggccg atcgtcggcc
     2581 tccccaaaca ggtaggtgtc ctgttcgcgc aagagctccc aagccgtccc ggcgtatgcc
     2641 gcggcgggcc cggcggcgtt gaagtccgaa acccagtccg ggtaacggtc caggtaatag
     2701 gtgctggtga cgaacccgcc cgtcgccttt gaaaaccagt acgcggtgcc ggaatgaccc
     2761 gccatggaga tggcgcccct atccttgacc gagacgccaa tggctttcgc gcgaccgttg
     2821 gtattgctgc gaagctcatc ggcaaaggtg gtcaccagga tcgcggcggg cgagcggcct
     2881 tcgctgcggg cggcgcgctg ggtgggatcg atctccgtgg catcgtcgac gtcggcggcc
     2941 tcggtgagca gccggtggtc cccatcctcg acgttgtagg tcgtgaaccc ggtttcccgg
     3001 tcgaaccaga gattgcccac catgccgtga acggaggggt gcgcgccggt agccagggtg
     3061 gcgtggccga cgatggtttc cgtgttggcg tgtgcgtgat gggcgttcgt gaagacgatg
     3121 cccttctccc acagatactt gaagccgcct ttgccgagcc gctcgtagta gcgggtcggc
     3181 agatcggccc tgagctgatc caccgtgatc tggagaatca gctttggccg gctggccgcc
     3241 tcgacggttg cgatgccggt aatcagcagc agcgccgcga gggcggccgc tgcgcgcaat
     3301 cgacgcgcta ccacgtcagt tcgtcggcgg tgacagcacg cccttctgat tgagctgctg
     3361 aaggatctgc agcgctttca gcgatcggta gttgatgccc gcggcgttca gggattggcc
     3421 ctcctggaac ggatagtcac ccatggtcga gaggaactcc tgaaccttgg cctggatggg
     3481 cacgaacagc cagatattgt ccgccatcca gcggagatac atggacgact cgtagtgcat
     3541 ctcttcaaac ggatccgaac gcagatgggt gattaccggc cagttggcca cttcccggat
     3601 acccgtcgcg atgttgccgt cgaaggtcgc aaagctgacc ttccagtccc gccagcgaac
     3661 cgcatccaga tcgccgttct ggccgaagta gaagatctcc tcgcgcggcg cgtccgcttc
     3721 gcccttgagg taaggcagga agttgtaacc atccgggtgc accctccagc gcttgccgtt
     3781 ggcccggtgg ccctttttca gcttctgcac aacgtcgttt tcgcccgccg cggccagcag
     3841 cgtcggcagc cagtcctcgt gggacatgat gtcgtggatc ttcgtcccgg gcttgatggt
     3901 ccccggccag cgcaccaccg cgggcacccg catgccgcct tcccaggtag tgcccttctc
     3961 gccgtggaac ggcgtcgtgc cgccgtccgg ccaggtgacc ttctcggcgc cgttgtcggt
     4021 gctgtagatg acgatggtgt tgtcggcgat gccgaggcga tccagctgat cgagaagcag
     4081 gcccacgtgg ccgtcgtgtt cggccatgcc gtccgggtag agccccattt ccgtgacccc
     4141 ctcggcctct gccttcaatc gcgtccacac gtgcatgcgc gtcgtgttca tccagacgaa
     4201 gaagggcttg tcggccttca ccgaacgatc gatgaagtcc atggcgctcc cgagaaactc
     4261 ctcgtccgcc gtctccatgc gctttcgcgt catgggcccg gtgtcctgga tctggccgtc
     4321 cgccgtgctc tttatcactc ctcgcggccc gaagcgcgcc cggaactccg ggtccttcgg
     4381 atagtgatag gtctccggct cttcctccgc attgaggtga tagaggttgc cgaaaaactc
     4441 atcaaacccg tgattggtcg gcaggtgctc gtcccgatca cccagatggt tcttgccgaa
     4501 ctggcccgtc gcgtagccgt ggttcttcag cagatcggcg atggtcgggc tccagtcggg
     4561 tatcccgtga tcggcgccgg gcatgccgat ggtcagcagg cccgtgcgga acgggtgctg
     4621 gcccaggatg aacgaagacc gtcctgccgt gcagctctgg tgggcgtagt aatgggtgaa
     4681 caatgcaccc tcctcggcga tccggtcgat gttcggcgtc tggtagccca tcatccccag
     4741 gttgtaggcg ctgatgttgc tccagcccac gtcgtcaccc cagatgacca ggatgttcgg
     4801 cttgtcctga gaccatgcgg acattgaggc gaacagcagc gcaagcagga tcgacgtggt
     4861 cgacgtccgt ctactcgatt tcatctgtga gatctccaaa tccgtgcgca gttagagtcg
     4921 gtgaaggcct gagcgtctgg ttattcttct tccgcgggtt cctgaatgcg caccagggcc
     4981 gatcccgctg cgcttttcca acgcttggca aaaccgcaca gcctagcgcc cggtctcggg
     5041 attgcaagtt tgtcggtggg acggtcaggc cgaaccagca gggagccgaa gttcatgacg
     5101 gggggccggt ttcatgaaga cggcgactct gacacgatag agagccgtcg aatcacactc
     5161 gccactccca ggaagtccgg acgcaatcct gatgagacac tggccgccga gccgcacgtc
     5221 acccatcggc gcagcagaca tcatcttcaa agcgatgctg gtgccggccg tgctgctggc
     5281 cgttgccgcc tgcacgcggg aagcggagcc acggtggctg ctccgcgcgc tgcctgggga
     5341 gtatcccgag atcgtctact tcgtgccgac gacctcccgg gcggtggcca tgactatcga
     5401 cgacggtctg gatcccgaga ccacgcctct gatcctcgag acgctcaaag ctcacggagc
     5461 ctcagccacg ttcttcgtcc tgagcgacag cattgccggc cgcgaggttc tgctgcagag
     5521 aatcctctac gagggccacg agatcggcca ccacatgacc gaggacgagg tcacggtctc
     5581 ccttaaaccg gctgcgatgg cagagaaatt ccatcgcgcc gcgcagacgc tcgacgcgtt
     5641 tgcgcccatc acctggtttc ggccgggctc cggccgttac aacgacgcgg tgctcgagct
     5701 gacgcgcgcg cggggctacc ggatcgcgct ggcctcggtg gcaccgctgg acaccgtgat
     5761 tcataattcg gcccgcatgg cgggcttcgt cgagcggatg atagagcccg gcagcgtggt
     5821 ggtgcttcac gacgtgggcg agcggggcag gagaaccgca gaaaccttaa ag
//
LOCUS       DBNHKV010000005         4088 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1346356, whole genome shotgun sequence.
ACCESSION   DBNHKV010000005 DBNHKV010000000
VERSION     DBNHKV010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1346356"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="AC16PP_00120"
     CDS             <1..356
                     /locus_tag="AC16PP_00120"
                     /inference="COORDINATES: protein motif:HMM:NF028121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HIR6480054.1"
                     /translation="VKALMAQVVELDEGYDHGGAHLYMGVFETLLPPSLGGKPEIGRA
                     HFERAVALSGGQHMLTKVMFAEQYARLVFDRELHDRLLNEVIETDPVVPGLTLMNVVA
                     RQQAQELLESADEYF"
     gene            343..1356
                     /locus_tag="AC16PP_00125"
     CDS             343..1356
                     /locus_tag="AC16PP_00125"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6480055.1"
                     /translation="MNISRRWLRAAVCAVSLLAAAAPAGAVVFKIATDAPDGSLWVSS
                     LRSAAEQVVEHTQGRVTFKFYPGGVMGDDKAVMRKIRSGQLHGAVMTVGGLTRTYTDI
                     QLYNMPMAFRSLAEVDHVRANMDQTLLNGLEEHGFVSFGIAEVGFAYAMTQERIATLE
                     EARAQKVWVPDGDEGAARSLESFGITPIPLSIADVLGGLQTGLIDSVTVPPVAAIVLQ
                     WHTQLDHVLDLPLLYVYGTLAVSKRQFNKVSDEDQAYMRQVLGELVREVNAQNRQDHE
                     RAVEVLEEQGLAWHAADPVAVAQWQDLADAASLELVEAGIVSAPIYEDMVRLLVEYRD
                     SVD"
     gene            1349..1810
                     /locus_tag="AC16PP_00130"
     CDS             1349..1810
                     /locus_tag="AC16PP_00130"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR6480056.1"
                     /translation="MIDVLSRWVRLVENGLLVGMLGVMIFLAAYQVIARNFFDSGLIW
                     GDSAVRIAVLWVTLLGAMVASRNDTHIRMDVISQFVDEAARKLLRRIGSVFTCVICGL
                     FAWYSYQFVVLDYQDEIIAFGRVPAWICEAIMPLGGAVMSFRYLLHVFRPA"
     gene            1807..3087
                     /locus_tag="AC16PP_00135"
     CDS             1807..3087
                     /locus_tag="AC16PP_00135"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6480057.1"
                     /translation="MIWVGIVCVLIAAVVGAPLFAVLMAAAMLGFVASDIDLTIVAIE
                     VYRIVDTPLLMVLPLFTYSGYLLAEARTSERLVNLVQSLFGWLPSGLAMVGFVACAVF
                     TALTGASGVTIVALGALLLPALARSGYAEKFGLGLVTASGSLGLLLVPSVPLILYGIV
                     AQQLDVGEPFTIFDLFVAGVLPTLLMLTMLIGWTLWRHRGGVIPRTPFSRQRLWEAVK
                     AARWELPLPVIVLGGIYSGYFAISEAAAVTAVYVTLAEVILYREVTWRELPAVMRNSM
                     VMVGGILLVLGVALAFTNFLVDAEVPQKLFEFMQENIQSKIGFLLLLNLTLLVLGAFL
                     DIFSALVIVVPLILPVAVGYGIHPVHLGIIFLANMQIGYFTPPIGMNLFIASYRFNKP
                     LHELYMASLPFMLVLLVALLLITYVPWFSLVFVE"
     gene            3096..3701
                     /locus_tag="AC16PP_00140"
     CDS             3096..3701
                     /locus_tag="AC16PP_00140"
                     /inference="COORDINATES: protein motif:HMM:NF017319.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6480058.1"
                     /translation="MLGILAAAIWLLAAAAHGQAEADTADPGEDPSPRIESFHAALLE
                     AMRSNDAYDVRFASLSPVVGEVFDVPTIARISLGRTWKSLDEPARAQFCELLEELITA
                     TYTDRFDSYSGQTFHLLSAEPARRGWVVKTELEKSDGERVSLDYYFRGSRVFNVVADG
                     VSDLSLRRADYNSIVKQEGYDRLLAHIRDSIAEYEAGGTDG"
     gene            3694..>4088
                     /locus_tag="AC16PP_00145"
     CDS             3694..>4088
                     /locus_tag="AC16PP_00145"
                     /inference="COORDINATES: protein motif:HMM:NF016236.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HIR6480059.1"
                     /translation="MAKALMLAVAVLLGGCASLEGPEYGPYDPFDKFNRGSYKFSDAV
                     DKNVLIPVVKGYQAVTPQWLRNGIGNVFSNLRELDSALNGYLQLKPGSGTTDLARVAL
                     NTTVGIGGFFDVAATGGLIYQEEDLGQTLA"
BASE COUNT          656 a         1237 c         1355 g          840 t
ORIGIN      
        1 gggtcaaggc gctcatggcg caggtggtcg agctcgacga gggctacgac catggcgggg
       61 cgcaccttta catgggcgtg ttcgagacgc tgctgcctcc ttctctggga gggaagcccg
      121 agatagggcg cgcccacttc gagcgggccg tcgcgttgtc cggaggtcag cacatgctga
      181 ccaaggtgat gttcgcggaa cagtacgcgc ggctggtatt tgaccgcgag ctgcatgacc
      241 gtctgctgaa cgaggtgatc gaaacggatc cggtcgtgcc gggtctaacg ctcatgaacg
      301 tggttgcacg gcaacaggca caggagcttc tggaatcggc ggatgaatat ttctagacga
      361 tggctccgcg cggccgtatg cgcggtttcc ctgctcgcgg cagcagcgcc tgccggagcg
      421 gtggtgttca agatcgccac cgatgcgccg gacggttccc tgtgggtgag ctcgctgagg
      481 tctgctgcgg agcaggttgt cgagcacacg cagggccgtg tcaccttcaa gttctacccg
      541 ggcggcgtca tgggagacga caaggcggtg atgcgcaaga tccgctccgg gcagctgcat
      601 ggggccgtaa tgaccgtggg tggcctgacc cgcacctaca cggacattca gctgtacaac
      661 atgcccatgg cgttcaggtc cctcgccgag gtcgatcacg ttcgcgcaaa catggaccag
      721 acgctactta acggcctcga ggagcatggc ttcgtttcgt tcgggatcgc cgaagtgggc
      781 tttgcctacg ccatgaccca ggaacgcatc gccaccctgg aagaagcgcg ggcgcagaag
      841 gtgtgggtgc cggacggcga cgaaggggcc gcgaggtcgc tggaatcttt cggcatcacg
      901 cccatcccgt taagcatcgc cgacgttctg ggagggcttc agaccggcct gatcgatagc
      961 gtgacggtgc ccccggtggc ggccatcgtg ctgcagtggc acacccagct cgatcatgtg
     1021 ctggatcttc ccctgctgta cgtctacggc acgctggcag tcagcaagcg tcagttcaac
     1081 aaggtctcgg acgaggatca ggcctacatg cggcaggtcc tgggcgagct ggttcgcgag
     1141 gtcaacgccc agaaccggca agaccacgaa cgtgccgtgg aggtgctcga ggagcagggc
     1201 cttgcgtggc atgctgccga tccggtcgcg gtggcccagt ggcaggatct cgccgatgcg
     1261 gcttctctgg agctggtcga agccggcatc gtatccgcgc cgatctacga ggacatggtt
     1321 cgccttctcg tggaataccg agacagcgtt gattgacgtc ctctcacgct gggtgcggct
     1381 cgtcgagaac gggctgctgg tcgggatgct cggcgtgatg atctttctgg cggcgtatca
     1441 ggtcatcgcc agaaacttct tcgattccgg tctcatctgg ggcgattccg ccgtgcgcat
     1501 cgccgtgctc tgggtgacgc tgctcggcgc gatggtcgct tcgcgcaacg acacccacat
     1561 tcgcatggac gtcatctcgc agttcgtaga cgaggcggcg cgaaagctgc tgcggcggat
     1621 cggcagcgtg ttcacctgcg tcatctgcgg cctgttcgcc tggtacagct accagttcgt
     1681 cgtcttggac tatcaggacg agatcatcgc gttcggccgg gtgccggcat ggatctgtga
     1741 agccatcatg ccgttgggcg gcgccgtgat gagcttccgt tatctcctgc acgtgttcag
     1801 gcccgcatga tctgggtcgg gatcgtctgc gtgttgatcg ccgccgtggt cggggcgccc
     1861 ctcttcgccg tgctgatggc cgccgcgatg cttgggttcg tcgcttcgga catcgacctg
     1921 acgatcgttg ccatcgaggt ctaccggatc gtcgacacgc ctctgctgat ggtgctgccc
     1981 ctgttcacat acagcggcta cctgctggcg gaggcgcgca cctccgagcg gctcgtgaac
     2041 ctcgtgcaga gcctctttgg ttggttgccg agcggtctgg ccatggtggg gttcgtcgcc
     2101 tgcgcggtgt tcacggcgct gaccggcgcg tccggcgtga ccatcgtggc gctgggcgcc
     2161 ctgctcctgc ctgccctggc gcggtcgggg tatgcggaga agttcgggct gggcctggtg
     2221 accgcttccg gcagcctggg gctgctgctc gttccctccg tgccgctcat cctctacggc
     2281 atcgtggctc aacagctgga cgtgggagag ccgttcacga tcttcgacct gttcgttgcc
     2341 ggcgtgctgc ccacgctgct gatgctgacg atgctgatcg gctggacact gtggcgccat
     2401 cggggagggg tcattccaag aacgcccttc tccaggcagc gcctgtggga ggcagtcaaa
     2461 gcggcgcgtt gggaattgcc gctgccggtg atcgtgctgg gcggcatcta ctccgggtac
     2521 ttcgcgatct ccgaagccgc ggcggtaacc gccgtttacg ttacgctcgc cgaggtgatc
     2581 ctctaccgcg aggtgacctg gcgcgagctg cccgccgtca tgcgcaattc catggtgatg
     2641 gtgggcggga tcctgctggt tctgggcgtg gcgctggcgt tcaccaactt tctggtcgat
     2701 gcggaggtgc cgcagaagct gttcgagttc atgcaggaga acatccaaag caagatcggc
     2761 ttccttctgc tgctcaacct gacgctgctg gttctcggcg ccttcctcga catcttctcg
     2821 gcgctggtca tcgtggtgcc gctcattctc ccggtggccg tgggctacgg cattcacccc
     2881 gttcacctgg gcatcatctt tctggcgaac atgcagatcg gctacttcac gccgccaatt
     2941 ggcatgaatc tgttcattgc cagctaccgc ttcaataagc cgctgcacga gctctacatg
     3001 gcgagcctgc cgttcatgct cgtgttactg gtcgctctgt tgctgataac ctatgtgccc
     3061 tggttcagcc tggtgttcgt cgagtgagag agtcaatgct tgggatcctt gcggcggcga
     3121 tctggctgct cgccgcggcc gctcacgggc aagccgaagc agacaccgca gatccgggtg
     3181 aggacccgtc tcccagaatc gaaagcttcc atgcggccct gctcgaggcg atgcgctcca
     3241 acgacgccta cgatgtgcgc tttgcgtcgc tgtcgccggt ggtgggggag gtgttcgatg
     3301 tccccaccat cgcccgcatc agcctcggac gaacctggaa gtccctggac gagcctgccc
     3361 gtgcgcagtt ctgcgagctg ctggaggaac tgatcaccgc gacttacacc gaccggttcg
     3421 acagctacag cggccagact tttcacctct tgagcgcgga gcccgcgcgc cggggctggg
     3481 tggtcaagac cgaactcgag aagtctgacg gcgagcgggt gtctttggat tactacttcc
     3541 gcggaagtcg ggtattcaac gtcgtggcgg acggggtgag cgatctctct ctgcgcaggg
     3601 ccgattacaa cagcatcgtc aagcaggagg gatacgatcg gctgctggcc cacattcgcg
     3661 atagcattgc cgagtacgaa gcgggaggaa cggatggcta aagcgctgat gctcgcggtt
     3721 gcggtgctgc tgggtggctg cgcctcgctg gaggggcccg agtacggccc ctacgacccc
     3781 tttgataagt tcaaccgcgg cagctacaag ttctccgatg cggtcgacaa gaacgtcctc
     3841 attcccgtgg tcaagggata ccaggccgtc accccgcagt ggctcagaaa cgggatcggc
     3901 aacgtgttct ccaacttgcg ggagctggac agcgcgctga acggctatct tcagctcaag
     3961 cccggatcgg gcaccaccga tctggccagg gtggctctga ataccaccgt cggcatcggc
     4021 gggttcttcg acgtcgccgc gacaggcggc ctgatctatc aggaggaaga tctggggcag
     4081 acgctggc
//
LOCUS       DBNHKV010000006         5091 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1275732, whole genome shotgun sequence.
ACCESSION   DBNHKV010000006 DBNHKV010000000
VERSION     DBNHKV010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1275732"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..2190)
                     /gene="ppk1"
                     /locus_tag="AC16PP_00150"
     CDS             complement(103..2190)
                     /gene="ppk1"
                     /locus_tag="AC16PP_00150"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003918.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR6480060.1"
                     /translation="MSANPSTDFAGLANPELYINRELSLLEFHRRVLAQAQEERVPLL
                     ERLRYLCISSSVLDEFFEIRVAGLKQQEAYRATQRGPDNLSPSEQLRRIFETVRELVE
                     EQYRVLNEDLLPSLDDQGIRILDPEEWSSKQRQWLKRYFKRELAPIMSPIALDPAHPF
                     PQPLNKSLTFIVTLEGEDAFGRRSGKAIVQAPRALSRVVRLPESCAATPNEFVLLSSI
                     IEVHVGDLFPGMKSTGCFQFRVTRNSDLFVDMEEVDDLLRALEGELSSRRFGDAVRLE
                     VARDCPDPLEAFLTGQFHLKPEDVYRCSGPVNLMRLATVPDLVDRPELKFPGFTPGIP
                     DRLRNANDMFEVIRQGDVLLHHPFESFAPFIDFLRQAAADPQVLSIRQTLYRTGTESA
                     VVEALRDAATNGKEVLVVIELRARFDEEANIELANYLQRAGAQVVYGVVGHKTHAKMS
                     MVIRREGRPLRRYVHLGTGNYHTRTTRLYTDYGLFTCDPDIGDDVQQLFQQLTSMGKT
                     SGLKKLLQAPFTMHSSMLVFIEREAENARAGRPARILAKMNSLIEPQVIQALYAASQA
                     GVKIDLVVRGVCGLRPGIEGVSENVTVRSIIGRFLEHTRVFYFENEGDPRVYLSSADW
                     MNRNFFDRVEACFPIEDASLRDRIYRELGMYLGDNCQSWMLAADGKYRRLVPPEGERR
                     SAQEGLLEELAGD"
     gene            2283..3011
                     /gene="cmoA"
                     /locus_tag="AC16PP_00155"
     CDS             2283..3011
                     /gene="cmoA"
                     /locus_tag="AC16PP_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003124239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HIR6480061.1"
                     /translation="MSSRDRVYRESKGQIVDFAFDEAVADVFPDMIRRSVPGYETVIP
                     ITGMLAARHLEHMPDERRLAYDLGCSLGATTTAVLDQLDERPARVIAVDNAAAMIARA
                     VDAIRDPRARFEVADVTMLEFEPAGVVLMNFVLQFIDPTQRVPLLKRIRQALEPDGLL
                     IVSEKLRFEDPYTQALFERAHLDYKRANGYSELEISQKRSALEKVMIVDTEAEHRSRF
                     EAAGFATTNRWFQCLNWVSYLVAP"
     gene            3008..3943
                     /gene="cmoB"
                     /locus_tag="AC16PP_00160"
     CDS             3008..3943
                     /gene="cmoB"
                     /locus_tag="AC16PP_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HIR6480062.1"
                     /translation="MIFEPVLDLIPEWRRLGPDLNARLSTKGHGDYPRWEAALAALPD
                     LQAEQAALSDRVTVSGNASEDQRRDLRESLMALHPWRKGPFSLFEVDIDCEWRSDWKW
                     LRVLPHLDSLSGRRVLDVGCGNGYYGWRMLEAGATAVFGVDPTLVFCMQHLAVSRYLS
                     GAANWVLPLRFEQLPPHPFDTVFSMGVIYHRRDPAEHARRLAEFTRPGGQVVVESLVV
                     EHGRSLKPVGRYARMRNVHVVPTTKRLARWLKDAGFVDPELVDLTPTTVAEQRATTWM
                     RFESLEDALDPKMRNRTIEGHPAPVRAVMVARKPV"
     gene            3965..4396
                     /locus_tag="AC16PP_00165"
     CDS             3965..4396
                     /locus_tag="AC16PP_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480063.1"
                     /translation="MRFETAYGLKTAKPQNTTMMLGIIPISFRFRGKIMRLEDMYHCF
                     REHPGTGLHPTQISRYTGLGLAEVNARLEATPELFVRLPKRPDGITRYRLTSATTAKA
                     PEDVEKFLRQAARRESLMLYAVGSMVLLILLIVVILVGPAV"
     gene            4397..>5091
                     /locus_tag="AC16PP_00170"
     CDS             4397..>5091
                     /locus_tag="AC16PP_00170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6480064.1"
                     /translation="MPELLDDKQRALREHAATFAREQLEPRRSMDNKQAREEIVRAAK
                     AAGFYAMTQPAEFGGNPASVLELTIVRDELAASNAPHADAVFGPGPGVLAGVGEPLAS
                     DYLGPVLAGTKQGAFGFTEPDDAPGPTSGRLESDELIVSGQKSYVTGGEQADFINTLV
                     QIEGHGPALVVIDTHAAGVIRERTFSSLDGSRHAAFRFEEVRVPRSHVIGKPGEGLPR
                     AMRQIGDTRLAIAA"
BASE COUNT          942 a         1668 c         1598 g          883 t
ORIGIN      
        1 aaacgccacc cgctcacccg cctggatctg gaccaggaag ccgactacct gtcggcggtc
       61 ggagtcggtc tgcgcatcga cgagaacgaa aatgccgcgc gatcagtcgc cggctaactc
      121 ttcgagcagc ccttcctgag cgcttcgacg ctcgccctcc ggagggacaa gccgccggta
      181 cttcccgtcc gctgcgagca tccaagactg gcagttgtcg ccgagataca tcccgagctc
      241 cctgtagatc cggtcacgca gggaggcgtc ctcaatggga aagcaggcct caacccggtc
      301 gaagaagtta cggttcatcc agtccgcgct ggacagatag accctcggat cgccttcgtt
      361 ctcaaagtag aacaccctgg tgtgctctag aaaccgcccg atgatcgagc gaacggtgac
      421 gttctcggac acgccctcga tacccgggcg cagtccgcat acgccgcgga ccacgaggtc
      481 gatcttcacc ccggcctgac tcgcggcgta cagcgcctgt atgacctgcg gctcgatgag
      541 cgagttcatc ttggccagta tccgcgccgg tcgccccgcg cgggcgttct cggcttcgcg
      601 ctcgatgaag acgagcatgg acgaatgcat cgtgaacggt gcctgcagca gcttcttcag
      661 cccgctggtc ttgcccatgg acgtgagctg ctgaaagagc tgctgaacgt cgtcgccgat
      721 gtcggggtcg caggtgaaca ggccgtagtc ggtgtaaagc cgcgtggtgc gcgtgtggta
      781 gttgccggtg cccagatgca catagcggcg cagcggacgg ccttcccgac ggatgaccat
      841 gctcatcttc gcgtgggtct tgtgccccac cacgccgtaa accacctgtg cgcccgcgcg
      901 ctgaaggtag ttggcgagct cgatgttcgc ctcctcgtcg aaacgcgcac gcaactcgat
      961 gaccaccagc acttccttgc cgttggtcgc cgcgtcccgc agcgcttcca ccaccgccga
     1021 ctccgtcccc gtgcgataga gcgtctgacg gatcgaaagc acctggggat cggcggccgc
     1081 ctgtcgcaga aagtcgatga aaggcgcgaa cgactcgaaa gggtgatgca gcagcacgtc
     1141 tccctgacgg atgacctcga acatgtcgtt ggcgttacgc agccgatcgg gaatacccgg
     1201 cgtgaacccc ggaaacttca gctccgggcg atcgacgagg tccggaacgg tcgccaggcg
     1261 catcaggttg acggggccac tgcagcgata gacgtcttcg ggcttgaggt ggaactggcc
     1321 ggtgagaaaa gcttccagag gatcgggaca gtcccgggcg acttcgaggc gaacggcatc
     1381 cccgaaccgg cgcgaggaaa gctcgccttc gagcgcccgc aacaggtcgt cgacttcttc
     1441 catgtcgacg aaaagatcgc tgttgcgcgt cacgcgaaac tgaaaacagc ccgtgctctt
     1501 catgcccggg aacaggtcac cgacgtgcac ctcgatgatc gacgagagca gaacgaactc
     1561 gttcggcgtg gcggcgcagg actccggcag gcgcaccacc ctggagagcg cgcgaggtgc
     1621 ctgaacgatg gctttgccgc tgcggcggcc gaaggcgtct tcgccttcga gggtgacgat
     1681 gaaggtgagg ctcttgttca gcggctgggg gaaggggtgc gcggggtcga gcgcgatggg
     1741 gctcatgatc ggcgcgagct cccgcttgaa gtaccgcttg agccactgcc tttgcttgct
     1801 gctccactct tccggatcca gaattcggat gccctggtcg tcgagactcg gaagcagatc
     1861 ttcgttgaga acccggtact gttcttcaac aagctctcgg acggtctcga agatgcgccg
     1921 cagctgctcg ctgggagaca ggttgtccgg tccccgctgt gtggcgcgat acgcctcctg
     1981 ctgcttgaga ccggcgaccc ggatctcgaa aaactcgtcg agcaccgagc tcgatatgca
     2041 cagatagcgc aagcgttcca agagcggcac gcgctcttcc tgggcctggg cgagcacgcg
     2101 gcgatgaaac tctagcagcg agagctcacg attgatgtag agctccggat tcgcgagccc
     2161 ggcgaagtcc gtggaagggt ttgcgctcaa ctgagactct gactaacaat tgacaccgca
     2221 ttttaccatg cgcgccggag gccaatgtta aagcttcgtg ttgccgacgg ggggccgcct
     2281 ccttgagttc ccgcgaccgc gtctaccgcg aatcaaaagg acagatcgtc gacttcgcct
     2341 tcgacgaagc cgtggccgac gtgtttcccg acatgattcg ccgctccgtc cccggctacg
     2401 aaaccgtgat cccgatcacg ggaatgctgg cggccaggca tctcgagcac atgcccgacg
     2461 agcgccgtct ggcttacgac ctgggttgct cgctgggcgc caccaccacc gccgtcctgg
     2521 accaactgga cgaacggccc gctcgcgtca ttgccgtgga caacgctgcg gccatgatcg
     2581 cccgagcggt cgacgccatc cgtgacccgc gggcgcgctt cgaggttgcc gacgttacca
     2641 tgctggagtt cgaacctgcc ggcgtcgtgc tgatgaactt cgtgctccag ttcatcgacc
     2701 ccacgcaacg ggtgccactc ctgaaacgaa tcaggcaagc gctcgaacca gacggcttgc
     2761 tcatcgtctc cgagaagctg cgcttcgaag atccttacac ccaggccctg ttcgagcggg
     2821 cccacctcga ctacaagcgt gccaacggat actctgagct cgagatcagc cagaaacggt
     2881 cagcactgga aaaggtgatg attgtcgaca ccgaagcaga gcatcgcagc cgcttcgaag
     2941 ccgccggatt cgcaaccacc aacagatggt tccagtgcct gaactgggtt tcctacctcg
     3001 ttgccccatg atctttgagc ccgtcctgga tctcataccc gaatggcgcc ggctcggccc
     3061 ggacttaaac gcgcgcctgt cgacgaaggg gcacggcgac tacccgcgct gggaagccgc
     3121 gctcgcagcg ctgcccgacc tccaggcaga gcaggccgcc ctcagcgatc gggtaacggt
     3181 gagcgggaac gcctccgaag accagcggcg cgacctgcgg gaatcgctga tggccctgca
     3241 tccgtggcgc aagggaccct tcagcctgtt cgaagtggac atcgactgcg agtggcgctc
     3301 ggactggaag tggctgcgcg tgctccccca cctggattcc ctttccgggc gccgcgtcct
     3361 cgacgtggga tgcggcaacg gctactacgg ctggcgcatg ctggaagccg gcgccaccgc
     3421 ggttttcggc gtggatccca cgctggtttt ctgcatgcag catttggcag tcagccgcta
     3481 cctgtccggc gcggccaact gggtgctgcc cctgaggttc gagcagcttc ctccgcatcc
     3541 gttcgatacc gttttcagca tgggcgtgat ttatcaccga cgggatcccg ccgagcacgc
     3601 ccgccggctt gcggagttca cgaggcccgg tggccaggtc gtggtggaat cactcgtggt
     3661 cgagcacggc cgatctttga agcccgtagg ccgctacgcg cgaatgcgca acgttcacgt
     3721 ggtgcccacg acgaaacggc tcgcgcgctg gctcaaggac gcgggcttcg tcgatccgga
     3781 gctcgtcgac ctgacaccta ccacggtcgc cgagcagcgc gcgacgacgt ggatgcgatt
     3841 cgaatccctc gaggacgctc tagatcccaa gatgcggaac cgaaccatcg aaggtcatcc
     3901 cgcccccgtc cgagccgtca tggtcgcgcg caagccggtc tgattcgcgc cggcgggcgt
     3961 ggaaatgcgc tttgaaaccg cgtacggtct caagacggca aagccgcaga acacaaccat
     4021 gatgctcggg atcattccca tcagttttcg cttccgcggc aagatcatgc ggctggagga
     4081 catgtatcac tgctttcggg agcatcccgg aaccgggctg catccaacgc agatatcccg
     4141 ctatacgggc ctgggtctgg ccgaggtgaa cgccagactg gaagcgactc ccgagctctt
     4201 cgtgcgcctt ccgaaacgcc ccgacggcat aacgcgctac cggctgacca gtgccaccac
     4261 ggcaaaggcg cccgaggacg ttgagaagtt cctccgccag gcggccaggc gcgaaagcct
     4321 gatgctttac gccgtgggaa gcatggtgct gttgattctg ctcatcgtcg tcatcctcgt
     4381 cgggcctgcc gtctagatgc ccgaactgct cgacgataag cagcgcgcgc ttcgcgagca
     4441 cgccgcgacc ttcgcccgtg agcagctgga acccaggcgc agcatggaca acaagcaggc
     4501 gcgtgaggaa atcgtccgcg ctgccaaagc cgccggtttc tacgccatga cccagcccgc
     4561 ggagttcggc ggcaacccgg cgtcggttct ggaactcacg atcgtcagag acgagttggc
     4621 ggcgagcaac gcgccccacg ccgatgccgt gttcgggccg ggtccggggg tgctggccgg
     4681 cgtgggagag cccctggcta gcgactacct tgggcccgtg ctggcgggca ccaagcaagg
     4741 cgcgttcggt ttcaccgagc ccgatgacgc ccccggacca accagcgggc ggctggaatc
     4801 agacgagctg atcgtcagcg ggcagaagtc gtacgtcacc ggcggcgagc aggccgactt
     4861 catcaacacc ctcgtgcaga tcgagggcca cggccccgcg ctggtcgtga tcgataccca
     4921 tgccgccggg gtgatccgcg agcgaacctt ctcgtcgcta gacggcagcc gtcacgccgc
     4981 ctttcgattc gaagaagtgc gcgtgccccg ctcgcacgtg atcggcaagc ccggcgaggg
     5041 gctgccgcga gccatgcgcc agatcggcga tacgcgcctg gccatcgccg c
//
LOCUS       DBNHKV010000007         5967 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_567707, whole genome shotgun sequence.
ACCESSION   DBNHKV010000007 DBNHKV010000000
VERSION     DBNHKV010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5967)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_567707"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="AC16PP_00175"
     CDS             complement(<1..217)
                     /locus_tag="AC16PP_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6480065.1"
                     /translation="MAILELVTLPPAFGLRNVSPFCLKAEMLLKSLDLPFAQSQLQDP
                     RKAPKGKLPYLLSDGVKIADSELITEHL"
     gene            complement(252..719)
                     /locus_tag="AC16PP_00180"
     CDS             complement(252..719)
                     /locus_tag="AC16PP_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6480066.1"
                     /translation="MQGPVRTMTLDEVRSFTGKEIGVSDWFELDQSKINQFADLTEDH
                     MFLHVDPEAAKATPFGSTIAHGLLTLSMMPVMAYQAMPGVSGTKMGVNYGYNKVRFMA
                     PVKSGKRIRGHFVVQGIEDKGDGRQQITHDVSIEIEGESKPALVAEWMTMVWM"
     gene            complement(1068..1655)
                     /locus_tag="AC16PP_00185"
     CDS             complement(1068..1655)
                     /locus_tag="AC16PP_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480067.1"
                     /translation="MAAIIAWFGVRMTRIAGDLAHNTGLGEALVGALFIGASTSLSGI
                     TTSVSVAAAGHAELAVSNGLGGIAAQTSFLALADIVYRRANLEHAAASAENLFMSAFL
                     LTLLGIHALALAIPDVGIFGAHPATVILVIAYVFGVHLLARTHDLPMWLPIRTADTPT
                     ETADHNVRRRHQRRAVLACQLDGDDRRAAHGSHLS"
     gene            complement(1897..2649)
                     /locus_tag="AC16PP_00190"
     CDS             complement(1897..2649)
                     /locus_tag="AC16PP_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480068.1"
                     /translation="MDWRTWLGLSLTIAWLLLGALYVSDQIGWSQFAALNADALGSFL
                     EGAFAPLAFLWLVIGYFLQQKELQQNTRALEAQAQEIQRSAEQAVIQSEKMAESEMHA
                     RQQAFLQIAESVKRQLGSIGGMLFISSQGSDAEGNVTAEEFSELFSMKAGQDPEIFSR
                     RLLESHTRARPERQLAMFYGTPVRARHSNNFIYTFERLLARAELVDTDNMIRDSLLGS
                     AHGFVYSLAKRHRAAAPEELADPSQTGTDIRF"
     gene            2839..3711
                     /locus_tag="AC16PP_00195"
     CDS             2839..3711
                     /locus_tag="AC16PP_00195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6480069.1"
                     /translation="MAFSSKPNFGVTIFPTDYTIQPAELAQAVEERGLDSLFFPEHTH
                     IPASRATPFPGGTELPRMYWHTHDPFVALGACAAVTSRIRLGTGICLVIERDPITLAK
                     EAASLDMLSNGRLVLGIGAGWNREEMENHGADYKNRWRIVREKVLAMREIWANEAAEY
                     HGEFVDFDPIWSYPKPVQPGGPPIWIGANSKWVFDRIADYADGWMPIGGIGSGNMERL
                     SEALAARGRKLEDVDMALFGAPTDYDQLMGRIEQGFDELVFALPDADADTVLPMLDDL
                     AELAGKARGAGRTA"
     gene            3871..4404
                     /locus_tag="AC16PP_00200"
     CDS             3871..4404
                     /locus_tag="AC16PP_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480070.1"
                     /translation="MDIDRVGSLMGNFGEFFGSIAVVVTLIYLALQVRQNSQALRIGA
                     RQHVLDRLSDAIRHIVSEPDTRAVITKAFNQIDDLTQDERVHFNHLMMLFSNNFYDAL
                     LLYRDSDLNDDAFNLVANAYVGSCATPGGNAWWKQMSEAAGDTGINFPPDFIEYVNRR
                     LEEDTGSGRTVGAVWQL"
     gene            4879..5199
                     /locus_tag="AC16PP_00205"
     CDS             4879..5199
                     /locus_tag="AC16PP_00205"
                     /inference="COORDINATES: protein motif:HMM:NF020317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR6480071.1"
                     /translation="MPKYLLEVDYSAAGNAGLVEGGGTARQQTVENMVNGLGGKVESF
                     YFTFGIRDAIVIADLPDNVTAAAISIACSATGSVAYKTTVLLTPAELDAASKKTVAYS
                     PPGA"
BASE COUNT         1246 a         1792 c         1798 g         1131 t
ORIGIN      
        1 ccaggtgctc ggtaatcagc tccgaatccg cgatcttcac tccgtccgag aggagataag
       61 gcagcttacc cttcggcgct ttgcgggggt cctgcagttg ggactgggcg aacggcaaat
      121 cgagcgattt gaggagcatc tccgccttca ggcagaaggg actgacgttg cgcaaaccga
      181 acgccggtgg aagggtgacg agttccagga tggccatgag ttttaccttc tgatccctca
      241 ggagggagaa cctacatcca caccatagtc atccattccg ccaccagggc tggtttggat
      301 tcgccttcga tctcgatgct cacgtcgtga gtgatctgct gccggccatc gcctttgtcc
      361 tcaatgccct gcacgacgaa gtgcccgcgg atgcgcttgc cgctcttgac cggagccatg
      421 aaccggacct tgttgtagcc gtagttgacg cccatcttcg tcccggacac gccgggcatc
      481 gcctgatagg ccatcacagg catcatcgac agggtcaaca gcccatgggc gatggtgctg
      541 ccgaagggag tcgccttggc tgcttccgga tcgacgtgca gaaacatgtg atcctcggtg
      601 agatcagcga actgattgat cttgctctga tcgagctcga accagtccga aactccgatc
      661 tccttgcccg tgaacgaacg gacttcgtcc agggtcatgg tgcgtacggg tccttgcatg
      721 gcggttgctc cttttggatt gactaaaaga tcaggtcgtt cacgatcagc gcagcccctg
      781 ctctatgttg gcgggcggat caggctggcc gattgcggat aacccgcaag atggtaatcg
      841 tgaggaccac atcctaacgc aaaacgaacg tcgtgccgca gcaccgcgcg tgctcgcccc
      901 attcgtcccc gtcgctcgat gcgctggaac ttcgagggtc gaatgcgaaa cgggatcgga
      961 gccctcagca ccgacgctag ggccagacgg acagggtgac cagccctccg agataaagca
     1021 gcagcaggat cacgctttcc ataccgatgt tccccggacc gtggcgctca cgatagatga
     1081 gacccatgag cagcacgccg gtcatcacca tcgagttggc aagccagaac tgctcgacgc
     1141 tggtgacggc ggcgaacgtt gtggtcggcg gtttctgtcg gcgtgtccgc cgttcttatc
     1201 ggaagccaca tcggcaagtc gtgagtgcgc gccagaaggt gcacaccaaa cacatacgcg
     1261 atgaccagaa tcacggtcgc cgggtgcgcg ccgaagatac ccacatccgg gatcgccagc
     1321 gcgagcgcat gaataccaag cagtgtcaga aggaacgccg acatgaacag attttcggcc
     1381 gaggcagccg cgtgctcgag gtttgcgcgg cggtacacga tatccgccaa cgccagaaag
     1441 gacgtttgag ccgcaatgcc ccccaaaccg ttgcttaccg ccagctccgc atgcccggcc
     1501 gctgccactg agaccgacgt ggtgattcca gacaacgacg tcgatgcgcc aatgaagagc
     1561 gcgcccacga gcgcttcccc cagcccggta ttgtgcgcaa gatctccggc gattctggtc
     1621 atcctgaccc cgaaccacgc gatgatcgca gccactccag cgaacgcggc cgcaaggaaa
     1681 gctgccggcc aactacccag atctatcagg agaggcctcc gaaaatccgg tcaaacgccg
     1741 atgttatgaa cgcaaggcgc atcatcgagc gacgctaacc gtccgttccg aaaaccatta
     1801 ccaatcgccg gcttcggcca gaatgccgtt gatgaatgct tgaaattccg gcgcgaatat
     1861 ccatgcgcca ctggcgatga ctccgagggc agcggctcag aatcgaatgt cggtgcccgt
     1921 ttggctgggg tcggcgagct cttcgggggc cgcggcgcgg tgccgcttgg caagggaata
     1981 cacaaaaccg tgggcgctgc ccagcaacga gtcgcgaatc atgttgtctg tatcgaccag
     2041 ctcagctcgg gcgagcaatc gttcaaaagt gtagatgaag ttgtttgagt gcctggctcg
     2101 aacgggcgtt ccgtagaaca tagccaattg acgctccggt cgcgcccggg tgtgcgattc
     2161 cagaaggcgc cgcgagaata tctccgggtc ctgtcccgcc ttcatggaga agagctcgct
     2221 gaactcctcg gccgtgacgt tcccctccgc gtcggagccc tggctggaga tgaacagcat
     2281 gcccccgatg gatccaagct gccgtttcac gctctcggcg atctggagaa aggcctgttg
     2341 ccgcgcatgc atttccgatt ccgccatctt ctcggactgg atcaccgcct gctccgccga
     2401 gcgctggatc tcctgagcct gcgcttccag ggcccgggtg ttctgctgga gctctttctg
     2461 ctgaagaaag taaccgatca ccagccagag aaaggctagc ggcgcaaagg cgccctcgag
     2521 aaagctgccc aacgcgtcag cgttgagcgc tgcaaactgg ctccatccga tctggtccga
     2581 gacgtacagc gccccgagca gcagccacgc aatcgtcaga gagaggccca gccaggtccg
     2641 ccaatccacg ggaagctcct gtcggctatc acgataactg attatcgctc cagcggctgc
     2701 agacggaaat cggtttcggt aacgctcctt catgagctca ttgcgcgcta ccaaggaccg
     2761 cttgcggcac gattcctcgc tcctcgggca cggcgccggg gtatgatgcc ggcattcact
     2821 ggaggggcaa gcagcatcat ggcgttctca tcaaaaccga atttcggtgt caccatcttt
     2881 ccaaccgatt acaccattca acccgcggag ttggcccaag cggttgagga acgaggactc
     2941 gatagcctct tctttccgga gcacacacac attcctgcat cgcgcgcaac gcccttcccg
     3001 ggcggcaccg agctgccccg gatgtactgg catactcacg acccgttcgt agcactcggt
     3061 gcgtgcgcgg ccgtgaccag ccgcatccgc ctgggcaccg ggatctgtct ggtcatcgag
     3121 cgtgacccca ttaccctggc caaagaggcc gcatcgctgg atatgctgtc caacggacgt
     3181 ctggtgctcg gcatcggcgc gggatggaat cgggaagaga tggaaaacca cggcgcggac
     3241 tataagaacc gctggaggat cgttcgggag aaagttctcg ccatgcgcga gatctgggcg
     3301 aacgaagctg ccgagtatca cggggagttc gtggatttcg atccgatctg gtcttacccg
     3361 aaaccggtgc agccaggggg gccgccgatc tggatcggcg ccaactccaa atgggtcttc
     3421 gaccgtattg cagactatgc cgacggctgg atgcccattg gcggtatcgg ttcgggcaac
     3481 atggaacggc tttccgaggc gctggccgcc cgcggcagga agctggaaga cgtggacatg
     3541 gcgcttttcg gggcgcccac ggattatgac cagctgatgg gtcgcatcga gcagggtttc
     3601 gacgagctgg tgttcgcgct gcctgacgcg gacgccgata ccgtgttgcc gatgctcgac
     3661 gatctggccg agctggccgg caaggcccgc ggggcgggcc gcacagccta acgcgtcgtc
     3721 gttcgcgcgt ttatcagccg gggaaatcga ccgaaagggc ctcccgcagc tccggcacga
     3781 acggttcctg cagctatgga ccgccggtac tacgcacagc cgcccgggtc tttagaattc
     3841 ggattcacgc aactcaaagg gaattcagcc atggatattg atcgggtcgg atcgctgatg
     3901 ggaaatttcg gagagttttt tggatcgatt gccgtagtcg tcaccttgat ctatctcgcc
     3961 ctgcaggtca ggcaaaattc acaggctctg cgaattggcg cccggcaaca cgtgctggat
     4021 cgcttgtcgg atgccatccg ccatatcgtc agcgaaccgg atacgcgagc ggtcatcaca
     4081 aaagccttca accaaatcga cgacctcaca caggatgagc gagtccactt caatcatctg
     4141 atgatgctct tctccaacaa cttttacgac gcgcttctgc tgtatcgaga ctccgacctg
     4201 aatgatgacg ccttcaatct ggtggccaac gcctacgtag gatcgtgtgc aacaccggga
     4261 ggcaacgcct ggtggaagca gatgtcggag gccgcggggg acacaggcat caactttccc
     4321 ccagatttca tcgagtacgt caatcgaagg ttagaggagg acaccgggtc cggaaggacg
     4381 gtgggggccg tctggcagct ctagcctcat cagatcccgc gaagtgacgg atcggggccg
     4441 agagcagtcg tcactgatcc atccaaccat tcccgccgct ggtggcaacc ggtcggattg
     4501 gggaaggcga ccatccaggt acggccagac cttggagaac accattgcgg tccgctgcca
     4561 agattttcgc caaccggatg actgaagaaa gaccagaaag ttgccgctga tgcagtcgcg
     4621 cgtttccagg tcaaggatcc cgtccttgcg gagcccagcg ccatcaaacc caaagactca
     4681 tcatcgttcc ggagatccca catgaggtca gcgaacgcca cctgtccgtt cgaagcggtc
     4741 atcaacgcaa aaggtaagga acaactgcta ttaggacgga cccggatgtc gcgtttagcg
     4801 ggcggctcat cgacttatag cctggcctgc gccgaccgag ttggagtatg ttggactgcc
     4861 ttaaacggag gtaacaccat gccgaaatac ctgcttgaag tcgattactc tgcagccggc
     4921 aacgccgggc ttgtcgaagg cggcggcacc gcgcgccaac aaacggtcga gaatatggtg
     4981 aacgggctcg gcggcaaggt cgagagcttc tacttcacct tcggcatccg cgacgccatc
     5041 gtcattgccg atttgcccga caacgttacc gcggcagcca tttcgatcgc gtgcagtgca
     5101 accggctcag tggcctacaa gacgaccgtg ctgctcactc ccgcagaact cgacgcggcg
     5161 agcaagaaga ccgtggctta ctcgccgcct ggggcgtgag atcaggtctc ggcacctgcc
     5221 gctgccgccc tcatgtgtta aacgctgggt tgttcaggcc gaccgcaatc agcgcctcga
     5281 gggctactct ctcgtcctcg gggagcttgc catcaaggac aaccatcgtc ccgcctgacg
     5341 atagcccacg agtagccccg tcgatcaccg ggtcgagatc gccgatgtag ccgggaggac
     5401 ctacggcaat caccccggac agagcgcgag gtcatagcga ctcgcatgct tgtcgcagac
     5461 ctgccgaatc tgcttcaggg aaagctgcgc tgccggttgg ccctgctttc tttgaagcgt
     5521 tgtaagggat cggggggctt gcgcataaga caaatagcgg gccggaatga gctggctggg
     5581 aagtacgatt gaagggtgat aggttgacga gggcccatgc gggcggtcgc aggcggggct
     5641 tttgctgttc tttgggtgct gagtcgctgc ggtaacactg ggttcccggg ttgggggacg
     5701 caaacggccg tcaagcgctc gttgtcctgg aaccctgtca gtcgggcgat gaagtgttta
     5761 tcgctcgacc actgggatga gccattacga agcacgccgt caaaccgaga ttaaagaagc
     5821 tgccagctga ggcggcacag ggagggcgct ttgaaaacga tatacagtga acggcatcgc
     5881 ttgcgggacg cgaaaacgga gctttacgga ggcgaactcg ttcaaccctt tgagcggcca
     5941 tcacgggccg acatggtgat cagggcg
//
LOCUS       DBNHKV010000008         4247 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1205374, whole genome shotgun sequence.
ACCESSION   DBNHKV010000008 DBNHKV010000000
VERSION     DBNHKV010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1205374"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /gene="argC"
                     /locus_tag="AC16PP_00210"
     CDS             complement(<1..832)
                     /gene="argC"
                     /locus_tag="AC16PP_00210"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR6480072.1"
                     /translation="MIRVFIDGQAGTTGLEIVQRLAARDDVELLEIDPAERKSPAARQ
                     RLFDSADVAVLCLPDDAAREAAVLADGRCRIIDASTAHRIQEGWCYGLPELGSNQRQA
                     LAAAERVANPGCYPQGFILMIRPLIGAGLLDPEQPLAVQALSGYSGGGRTLIEARRAF
                     GSQEAERRNTEPYALSLKHKHVPEMQAYSGTKIAPLFTPSVGQFYQGMLVGVPLFCSQ
                     LRKGADYDGIYHILEEAYALEPFVDVLEPGAETALTDGFLNATACNGTNRLEIMLFGH
                     S"
     gene            complement(984..1760)
                     /locus_tag="AC16PP_00215"
     CDS             complement(984..1760)
                     /locus_tag="AC16PP_00215"
                     /inference="COORDINATES: protein motif:HMM:NF018649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR6480073.1"
                     /translation="MGLFELYGVAALTVWVMVTTLWAVSVPTKDASIIDMFWGPLFVA
                     IAWVLLPVYGGGVQAKPYLVMLLVTLWGLRLAYHLIGRNLAAGEDVRYARWRASGGPK
                     WWLRTYHRIFLLQGFLALLVATPILASFYAPRDLTLLNLAGVLIWAAGFVFELVADVQ
                     LSRFKADPANRGQLLNTGLWGLSRHPNYFGDALQWWGLSIVALSGAAWWALLGPVVMT
                     MIFLVLTADILEQGMSRRHPDYEEYVSNTPKFVPRLPGRD"
     gene            complement(1858..2511)
                     /locus_tag="AC16PP_00220"
     CDS             complement(1858..2511)
                     /locus_tag="AC16PP_00220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6480074.1"
                     /translation="MTDLLDCVEIDPGGTAGSVIWLHGVGADAHDFEPVVPLLPLNRP
                     LRFVFPNAPVRPVTINAGMEMRAWYDIDPGNPLSGDSDIQSSAKAIAGLVEREHDRGV
                     PYDGITLAGFSQGGVIALELGLSFPHRLAGIMALSTYVPDHEHLVDRIGFANVDAPIF
                     MAHGIGDPMIPITRAITSREALLAMNYEVEWHEYGMGHQVCPEEIADIGTWLNTIYR"
     gene            2645..>4247
                     /locus_tag="AC16PP_00225"
     CDS             2645..>4247
                     /locus_tag="AC16PP_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009686258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR6480075.1"
                     /translation="MEDGLDRQASRELTRLLPALGFLRPYVRQVVFASIALVVTAGVT
                     LSIGQGMRLVIDEGLGSGSAAVLVQSISIFAVLMVVLTAGTFLRFYFVSWVGERVSAD
                     IRQAVFAHLIRLHPGFFETNLPTEIQSRITTDTTVLQTVIGSSVSIALRNVLMFVGGL
                     VLLFVTNPKLSLIVVASVPFVVAPVILFGRRVRALSRDSQDTLAEVGSFAGESLRNIK
                     IVQSFNHEGLDIEAFRQRVERAFQVAVRRILQRSWLVALVMMLVLGAIATMLWVGGRD
                     VLAGQTSPGELAAFIFYAFIVAGSVGAISEVYSDLQRAAGAMERLVELLNSPSLLTDP
                     ESPVLPAAREPSVAVSPGGRLTVRNLGFAYPSRPDAPVLRGLSFAVAPGEMVALVGPS
                     GVGKSTLFDLLQRFYDPDAGQILLDGVDLRELRLADVRERVGLVPQDPVLFAGTVESN
                     LIYGAPDAGDDEKSRALELAHASAFIEALPEGLATVVGEGGVGLSGGQKQRLAIARAL
                     LKRPEILLLDEATSALDAESEQHIRA"
BASE COUNT          695 a         1350 c         1432 g          770 t
ORIGIN      
        1 ccgagtgccc gaacagcatg atctcgagcc ggttggtgcc gttgcaggcg gtggcgttga
       61 gaaaaccgtc cgtcagggcg gtctccgcac cgggctcaag cacgtcgacg aaaggttcca
      121 aggcgtaggc ttcctcgagg atgtgataaa tgccgtcata gtctgcgccc ttgcggagtt
      181 ggctgcaaaa caacgggacc ccaaccagca tgccctggta gaactggccc accgacggcg
      241 taaacagcgg ggcgatcttc gttcccgagt aagcctgcat ctcggggacg tgcttgtgct
      301 tgagcgacaa agcatagggc tcggtattgc ggcgctcggc ctcttgggaa ccgaaggccc
      361 tgcgagcctc gatgagcgtg cgccctcccc cggaatagcc ggaaagcgcc tgcacggcca
      421 ggggctgctc agggtccagc aggcctgcgc cgatgagggg gcggatcatg aggatgaagc
      481 cctggggata gcaaccggga ttggcgaccc tttctgccgc ggcaagggcc tggcgttggt
      541 tggatcctag ctcaggcaga ccgtagcacc atccctcctg aatccggtgg gccgtgctgg
      601 cgtcgatgat gcggcagcgg ccgtccgcca gaactgcggc ctcgcgcgcc gcgtcgtcgg
      661 gcaggcacag gacggcaacg tcggcagagt cgaacagccg ctgtcgggcc gcgggtgatt
      721 tgcgctcggc gggatcgatc tccagcaact cgacgtcgtc gcgcgccgcc aggcgctgga
      781 cgatctcgag gcccgtggtg ccggcctgtc cgtcgatgaa taccctgatc atggtgccga
      841 ggtccttccc cgcggggttg tttcttaggg gcgcgcgatt ttaggcaata tcggcccgat
      901 gtcacagcgg cgcgacggaa aagatggccc ctcggctgtt ccggcacccg tttgcccgtc
      961 tgtgcccccc gcggatcccc gggtcaatcg cggccgggga gccggggaac gaacttgggc
     1021 gtgttggata cgtactcctc gtaatcggga tgccggcgag acatcccttg ctcgagaatg
     1081 tcggcggtga gcacgagaaa tatcatggtc atgaccacag ggccgagcag cgcccaccac
     1141 gcggcgcccg acagcgcgac gatggacaga ccccaccact gcaacgcgtc gccgaagtag
     1201 ttggggtggc gggacaggcc ccacaggccc gtgttgagca gctggccccg gttggcagga
     1261 tctgccttga atcggctgag ctgaacgtcg gcgacgagct cgaagacgaa accggcggcc
     1321 cagatcagga cgccggcgag gttcagcagc gtcaggtccc ggggtgcgta gaagctggcg
     1381 aggatgggcg tggcgacgag cagggcgaga aatccctgca ggaggaagat tcggtggtag
     1441 gttctcagcc accacttcgg tccgcctgac gctcgccatc gggcatagcg gacgtcctcg
     1501 ccggcggcga gattgcgacc gatcaggtga taggccagcc gaaggcccca cagggtgacg
     1561 agcagcatga ccaggtaagg cttggcctgc acaccgccac cgtagacggg cagcagcacc
     1621 caggctatgg ccacgaacag cggcccccag aacatgtcga tgatgcttgc gtccttggtc
     1681 ggcacgctga cggcccacag tgtcgtgacc atcacccata cggtcagtgc ggcgaccccg
     1741 tacagctcga acagtcccat gtctatctcc actctatctc cgctcgatcc tcgcggccgt
     1801 tactgaattc gcgggtgcgc cttagggcca cccgagttct cgctctttcc cgacgcatca
     1861 gcggtagatg gtgttcagcc acgtgccgat gtcggcgatc tcctccgggc acacctggtg
     1921 gcccatgccg tactcgtgcc actcgacctc atagttcatc gccagcagcg cctcccgcga
     1981 ggtgatcgca cgggtgatgg ggatcatcgg atctccaatg ccgtgtgcca tgaagatggg
     2041 ggcgtccacg ttggcgaacc cgatccgatc caccaggtgc tcgtgatcgg gcacgtaggt
     2101 ggagagtgcc atgatcccgg ccagcctgtg ggggaacgac aggcccagct ccagcgcgat
     2161 gacgcccccc tgggaaaacc ccgcgagcgt gatgccatcg tatggcacgc cgcggtcgtg
     2221 ctcgcgctct accagccccg cgatcgcctt ggccgaggat tgaatgtccg aatcgccgga
     2281 cagcgggttc ccgggatcga tgtcgtacca ggcgcgcatt tccatgccgg cattgatggt
     2341 gaccgggcga acgggcgcgt tggggaacac gaagcgcagc ggccggttca gcggcagcag
     2401 gggcaccact ggttcgaagt cgtgcgcgtc ggcgcccacc ccgtgcagcc atatcaccga
     2461 accggcggtt ccgcccgggt cgatctcgac gcagtccagc agatcagtca ccgtatttcc
     2521 cgacgggcga gctccgcggc agctaaacaa gatctcgggg ggaaagcaat ctccgtttct
     2581 ctcgccaggg tcggtcaacg cggcggccct acgttcgcgg ccctgcaggt gagatactac
     2641 ggccatggag gacggactcg acagacaggc atcacgggag ctgacgcggc tgctgcccgc
     2701 tctgggcttt ctgcgcccgt atgttcgcca ggtggtattc gcgagcattg ccctcgtcgt
     2761 gacggcgggc gtgacgcttt ccataggcca ggggatgcgc ctggtcatcg acgaaggcct
     2821 gggcagcgga tcggccgccg tgctcgtgca gagcatatcg attttcgccg ttctcatggt
     2881 ggttctcacg gccgggacgt ttctgcgttt ctacttcgtc tcttgggtgg gagagcgcgt
     2941 cagcgcggac atccgccagg cggtcttcgc tcatctcatc cgccttcatc caggtttctt
     3001 cgagacgaat ctgcccaccg agatccagtc gcggatcacc accgacacga ccgtgctgca
     3061 gaccgtcatc ggatcgtccg tctccatcgc cctgcgcaat gtcctgatgt tcgtcggcgg
     3121 gctggtgctc ctgttcgtga ccaatccgaa gctctcgctg atcgtggtgg ccagcgtgcc
     3181 cttcgtggtg gccccggtga tcctgttcgg ccggcgcgtg cgggcgctgt cgcgcgacag
     3241 ccaggacacc cttgccgagg tgggaagctt tgccggcgag tcgctgcgca acatcaagat
     3301 cgttcagtcc ttcaatcacg aagggctgga catcgaagcg ttccggcagc gggtggagcg
     3361 ggcttttcag gttgccgtcc gccgtatcct gcagcgctcg tggctggtcg ccctggtcat
     3421 gatgctggtg ctcggggcca tcgcgaccat gctctgggtg ggggggcggg acgtgctcgc
     3481 cggccagacc tcgcccggag agcttgccgc cttcatcttc tatgccttca tcgtcgcagg
     3541 ctcggtcggt gccatcagcg aggtctacag cgacctgcag cgagctgccg gtgccatgga
     3601 gcgcctcgtg gagctgctga actcgccatc gctcctgacc gatcccgagt cgcccgtcct
     3661 gccggctgcc cgggagccgt cggtggcggt ttctccgggt ggccgcctga cggtccggaa
     3721 cttggggttt gcatatccga gccggccgga tgcgcccgtg ctgcgcggtc tgagctttgc
     3781 tgtggccccg ggagagatgg tcgccctggt ggggccgtcg ggcgtgggaa agagcacgct
     3841 gttcgatctg ctgcagcgtt tttacgatcc ggacgccggg cagattctgc tcgacggcgt
     3901 cgacctgagg gagttacggc ttgccgacgt tcgtgaacgg gtgggtttgg tgccccagga
     3961 tcctgtgctc tttgctggta ccgtagagag caacctgatc tacggcgcgc ccgacgcggg
     4021 cgatgacgaa aaatcccgcg cgctcgagct cgcccatgcc agcgccttca tcgaggctct
     4081 gcccgagggc ctggccacgg tggtgggcga gggtggcgtc gggctctccg gagggcagaa
     4141 gcagcggctg gccatcgccc gcgcccttct gaaacgaccg gagatcctgc tgctcgatga
     4201 ggcgaccagc gcgcttgatg ccgagagcga gcagcacatc cgggcga
//
LOCUS       DBNHKV010000009         5913 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_638669, whole genome shotgun sequence.
ACCESSION   DBNHKV010000009 DBNHKV010000000
VERSION     DBNHKV010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_638669"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="AC16PP_00230"
     CDS             complement(<1..593)
                     /locus_tag="AC16PP_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009868041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIR6480076.1"
                     /translation="MSKRVKILVPIPMDAAGVANRASQLPADRIRDGYEVEFVAVSWG
                     AALGDSYYDTMLMDFSVFEAGLKAQEEGFAAICIDTVSDSGLYALRSRLDIPVFGPGQ
                     AALHAACMLGHKFSIITMWDEWFHLYTKILKEYVLEQRCASLRSIKTRPDLEELLAGK
                     EEVIFKKLEEESLRAIEEDGADVIVLGSTTMHQSHAHL"
     gene            complement(611..2461)
                     /locus_tag="AC16PP_00235"
     CDS             complement(611..2461)
                     /locus_tag="AC16PP_00235"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6480077.1"
                     /translation="MPQREVIEGLYSGATYSPYAERSFPSEVFWGETHLHTGLSLDAG
                     LFGNILGHEDAYRLARGEQITSSSGLPIKLGRPLDWLVIADHSDMMGIATDIQRGAPN
                     IRAVPKGREWSDGFKQGGQAAAEAAFDLIQHFAQFKVPPELVEQYSPGSPIYSGVWDS
                     IIETADRFNDPGRFTAFIGFEWTSVPKGFNLHRNVVLRDDGRRASQVMPLTTQPPLGT
                     TDPLDLYQWLEDYESKTGGQALALAHNGNLSNGWMFPMGKTYAGGRVNKEYVQLRAKW
                     EPLYEVTQIKGDGEAHPALSLNDEFADYGTWDKGNLDLSELKKPEMLSGEYAREALKN
                     GLVLEEKFGTNPYKFGMVGATDSHTALPTAEEDNFFGKSTSVEPSATRVAHPFVASEL
                     ATIEGYELVAAGYQGVWATENTRAALFDAMERKETYATTGPRMIVRFFGGWDYNENDL
                     RSRAPAFAGYEKGVPMGGDLTAAPDGKSPTFMAYAVRDPVGANLDRLQIIKGWLDERG
                     KTHEKVYDVTWSGGRQLDANGKLPAVGNTVDIEAANWTNTIGASELAAVWTDPDFDAQ
                     ERAFYYVRVLEIPTPWWALYDKVRLGAEIPEGVELTHQERAYTSPIWYTP"
     gene            2760..3620
                     /locus_tag="AC16PP_00240"
     CDS             2760..3620
                     /locus_tag="AC16PP_00240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR6480078.1"
                     /translation="MRYESPASTKEAVALLAQEKGSAFVLAGGTDLLVRMKAELIEPD
                     LVVDIKRIPAMTDIRVTEKGFRIGAAVSAAQLGRHKALCKAWPGVVEAANLIGSDQIQ
                     GRCTVVGNLCNASPAADSVPALVAAGAKAVIVGRNGRRTVPVEKVITGPGRTSLEKGE
                     VIEALTLPKPASRTGDAYLRFTPRTEMDIAVVGVGISLALERGTVKKARVCLGAVAPT
                     PILVPAAARALIGSKLDDVALARLATACSAACNPIDDKRGTAAYRIRVAGVLARRAAT
                     IAFQRAGGKA"
     gene            3617..4105
                     /locus_tag="AC16PP_00245"
     CDS             3617..4105
                     /locus_tag="AC16PP_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013923.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR6480079.1"
                     /translation="MSNAVLVSTTINGDTVEFACPADEVLLDSLRDRVGLTGVKEGCG
                     TGDCGACSVTLDGELVCSCLVLGVEADGRAIGTVEGLTENGDLHPLQQNFIDHAALQC
                     GICTPGFLVAAKALLDRNPNPTETEIRYALAGNLCRCTGYDKIVRAVQASAKDLRKAK
                     KR"
     gene            4133..>5913
                     /locus_tag="AC16PP_00250"
     CDS             4133..>5913
                     /locus_tag="AC16PP_00250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6480080.1"
                     /translation="MAHDNSYTGQKFQVIGNRFARPDGVDKVTGRARYGADANAPGQL
                     VARVLRSPHAHARIKKIDTSKAEKLSGVKAVITSDDLPDLTGGNRGMTDILENCMARG
                     RALYDGHAVAAVAAIDEQTARKALKLIKVTYQMLPHVTDVDEAMQPEAPVIHDWVFTG
                     GMEPKPTKPSNVANRTEMGHGDVDAGFAAADVIVERSYKTEQTHQGYIEPHACLASVN
                     PDGSAELWVTTQGPFSYRNVCAGLLGLDIAKLKVTASEIGGGFGGKTHVWAEPLALAL
                     SRKANRPVKLVMTREEVFRATGPTASTSIDVKIGAKKNGEITAATLDMRYQGGAFPGI
                     WGMLGCMTGFACYDIKNQRSVGWDVIVNRPKVAAYRAPSAPMAAFAVESTMDIVAGEV
                     GMDAVDFRLKNVAREGSQAPYGPIFGPIGIESALQEVKKHPQMKARLKKNQGRGVACG
                     FWFNIGGQTCVDLNIGEDGTVNLAVGTVDVGGARSSLGMIAAEELGISYDDVKVHVAD
                     TASLGHNDTTEGSRGTFSSGMAAIFAARDAIEVLKDRAAQMLDVPRDAIVWEKGEARA
                     KGTQYSNLPKLSLREIAAKAARTGGPIA"
BASE COUNT         1119 a         1898 c         1899 g          997 t
ORIGIN      
        1 aggtgtgcgt gagactgatg catggtcgtg gatccaagca cgatcacgtc ggcgccgtcc
       61 tcctcgatgg cccgcaggct ctcctcctcc agcttcttga agatcacttc ctccttcccg
      121 gccagaagct cctccagatc gggacgggtc tttatcgaac gcagcgaggc acacctctgc
      181 tcgagcacgt actccttgag gatcttcgtg tagagatgaa accactcgtc ccacatcgtg
      241 atgatcgaga acttgtggcc cagcatgcag gccgcatgaa gcgccgcctg gccggggcca
      301 aagaccggaa tgtcgagccg cgagcgcagc gcataaaggc cggagtcgct caccgtatcg
      361 atgcagatgg cggcgaagcc ctcctcctga gccttcaggc ccgcctcgaa cacgctgaaa
      421 tccatgagca tcgtgtcgta gtaggaatcc cctaacgccg cgccccagct gaccgccaca
      481 aactccacct cgtaaccatc gcgaatgcga tcggccggca gctgcgatgc ccggttggca
      541 acgcccgccg cgtccatcgg tatcggcacc agaatcttga cccgcttact catgctcgac
      601 tccgtctggc tcagggcgtg taccagatgg gcgaggtata cgcccgctcc tggtgcgtca
      661 gctccacgcc ctcgggaatt tcggcgccga ggcgcacctt gtcgtaaagc gcccaccacg
      721 gcgtagggat ctccagcacc cgcacgtaat agaaggcgcg ctcctgcgca tcgaagtcag
      781 ggtccgtcca gacggcggcc agctccgagg cgccgatggt gttggtccag ttggccgctt
      841 cgatgtccac cgtgtttccc acggcgggaa gcttcccgtt ggcatccagc tgacggccgc
      901 cggaccaggt gacgtcgtag accttctcgt gggtcttgcc gcgctcgtcg agccagccct
      961 tgatgatctg caggcgatcg aggttggccc ccacgggatc ccggaccgcg taggccatga
     1021 aagttggcga cttgccgtcc ggcgccgctg tcagatcccc ccccatgggc acgcctttct
     1081 cgtagccggc aaaggcgggc gcccggctgc gcaggtcgtt ctcgttgtag tcccagccac
     1141 cgaagaagcg cacgatcatg cggggacccg tggtggcgta ggtctctttt cgctccatgg
     1201 cgtcgaacag cgcggcgcgc gtattctcgg ttgcccacac accctggtag ccggcggcga
     1261 ccagctcgta gccctcgatg gtggccagct ccgacgctac gaaaggatgg gcgacacgag
     1321 tcgccgacgg ctcgacgctg gtcgacttgc cgaagaagtt gtcctcctcc gccgtgggaa
     1381 gggcggtatg gctgtcggtg gccccgacca tgccgaactt gtaagggttg gtgccgaatt
     1441 tctcctcgag caccagcccg ttcttcaacg cctcgcgagc atactcgccg ctcagcattt
     1501 caggcttttt gagttcggac agatcgaggt tgcccttgtc ccatgtgccg tagtcggcga
     1561 actcatcgtt gagcgagagg gccgggtgcg cttcgccgtc tcccttgatc tgggtgacct
     1621 cgtaaagcgg ctcccacttc gcgcgcagct gcacgtactc cttgttcacg cggccccccg
     1681 cgtatgtctt gcccatggga aacatccaac cgttggacag gttcccgttg tgcgccagag
     1741 ccagcgcctg accgccggtt ttgctctcgt agtcctccaa ccactggtag agatccagag
     1801 ggtcggtggt ccccaacggc ggctgggtgg tcaggggcat cacctgactg gcccgtcggc
     1861 cgtcgtctcg cagcaccacg ttgcgatgca ggttgaaacc cttgggaacc gacgtccatt
     1921 cgaacccgat gaaagcggtg aaacgcccgg gatcgttgaa gcggtcggcc gtctcgatga
     1981 tgctgtccca cacgccggaa tagatgggcg aaccagggga atactgctcg accagctccg
     2041 ggggaacctt gaactgcgca aagtgctgaa tgagatcgaa cgcggcctcg gcagccgcct
     2101 gccccccctg cttgaacccg tcgctccact cgcggccttt cggcaccgcg cgaatgttgg
     2161 gggcgcctcg ctgaatgtcg gtggcgatgc ccatcatgtc ggagtggtcg gcgatcacca
     2221 gccagtcgag gggtcggccc agcttgatgg gcagaccact cgaggacgtg atctgctcgc
     2281 ctcgagccag gcgataagcg tcttcgtggc ccagaatgtt tccgaacagc ccagcgtcga
     2341 gagacaggcc ggtgtgaagg tgggtctctc cccagaagac ctcgctcggg aacgagcgct
     2401 cggcgtaagg cgagtatgtg gccccagagt agagcccttc gatgacctcc ctttgcggca
     2461 ttccgatgtc cgaggccgat gccgtcagag gtgccagaag gaacaaatac agtgcgatag
     2521 gacgtgtcat gccggactcc tcatcgaggg cgtgttaaat ttttttcagc ctgcagccag
     2581 cgcgcgccga ctgacgtttt cccccgatgc tcgaatggta gacgattcgt gtggacgaat
     2641 gaatcgatcc cggtcaatca gcttcgctcg aagacgagat cgttgccgga aggcggcgcc
     2701 gcggatagga tgcggagatc gtctgatccc tcaccgactt cagccacagg acactcacga
     2761 tgagatacga atccccagcc tcgacgaaag aagccgtcgc gctgctggcc caggaaaaag
     2821 gctcagcctt cgtgctggcg ggcggcaccg acctgctggt caggatgaag gccgagctga
     2881 tcgaacccga cctcgttgtc gacatcaaac gcattccagc aatgaccgac atccgggtga
     2941 ccgagaaggg gtttcggatc ggagcggccg tttccgctgc ccagctcggc cggcacaaag
     3001 ccctgtgcaa ggcgtggccg ggcgtggtgg aggcggccaa tctcatcggc tcggatcaga
     3061 ttcaggggcg ctgcacggtg gtcggaaacc tgtgcaacgc atcgcccgcg gccgacagcg
     3121 tgccggccct ggtcgccgcc ggggcaaagg ccgtcatcgt gggccggaac ggacgaagaa
     3181 cggtaccggt ggagaaggtg atcaccgggc cgggcagaac ctccctggaa aaaggcgagg
     3241 tcatcgaagc cctcacgctc ccgaaaccgg ccagccgcac gggcgacgcc tacttgcgtt
     3301 tcacccctcg aaccgagatg gatatcgccg tggtgggcgt cgggatcagc ctggcgctgg
     3361 agcgaggcac ggtcaaaaaa gcgcgcgtct gcctgggggc ggtggcgccc acgccgattc
     3421 tggtgccggc ggcggcaaga gcactgatcg gatccaagct cgacgacgtc gcgctggcca
     3481 ggctggccac agcatgctcc gcggcctgca accccatcga cgacaaacgc ggcacggcgg
     3541 cttaccgcat ccgcgtggcg ggagtgctgg cgcggcgcgc ggcgacgatc gcgtttcaac
     3601 gcgcaggagg taaggcatga gcaacgcggt tttagtatcc acgaccatca acggcgacac
     3661 ggtcgagttc gcctgcccgg cagacgaggt gctgctggac tccctgcgcg accgggtcgg
     3721 tctgaccggc gtcaaggagg gctgcgggac gggcgactgc ggtgcctgca gcgtgacgct
     3781 ggacggtgag ctggtgtgct cctgcctggt gctcggcgtg gaagccgacg gccgcgccat
     3841 cggcacggtg gaaggtctca ccgagaacgg cgacctgcac cccttacagc agaacttcat
     3901 cgatcatgcc gctctgcagt gcggcatctg cactccgggg ttcctcgtcg cggcgaaagc
     3961 gctgctagac agaaatccga acccgacgga gacggaaatt cgctatgccc tggcgggcaa
     4021 tctgtgccgc tgcaccggct acgacaagat cgttcgcgcg gtgcaggcct ccgccaagga
     4081 tctgcgcaaa gccaagaaac gttaaccact gacgcgacga taggaattcg acatggccca
     4141 cgacaacagc tacaccggac agaaattcca ggtgatcggc aaccggttcg cccggcccga
     4201 cggcgtcgac aaggtaaccg gccgggctcg ttacggtgcg gacgccaatg caccgggtca
     4261 actggtcgcc cgggttctcc gctcacctca tgcccacgcc aggatcaaga agatcgacac
     4321 gtccaaggcg gagaaattat ccggcgtcaa agccgtcatc acgagcgacg atctgccgga
     4381 cctgaccggc gggaatcgcg ggatgacgga catcctggag aactgcatgg cccgcgggcg
     4441 ggcgctctac gacggtcacg cagtggccgc ggtggccgcc atagacgagc agacggcccg
     4501 caaggcgctg aagctgatca aggtgaccta tcagatgctt ccccacgtca ccgacgtgga
     4561 cgaagcgatg caaccggagg cgcccgtgat tcacgactgg gtgttcaccg gcggcatgga
     4621 gcccaagccg acaaaaccct cgaacgtcgc caaccgcacg gaaatgggcc acggcgacgt
     4681 ggatgccggg ttcgcggcag ccgacgtgat cgtggagcgg tcgtacaaaa ccgagcagac
     4741 ccaccagggc tacatcgagc cccacgcttg cctggcatcg gtcaacccgg atggctcggc
     4801 ggagctgtgg gtaaccaccc agggaccgtt ctcgtaccgg aacgtgtgcg ccggcctgct
     4861 gggcctggac atcgccaagc tgaaggtgac ggcctcggag atcggcggcg gcttcggcgg
     4921 taagacccac gtctgggctg agcccctggc tctggccctt tcccgcaagg ccaaccggcc
     4981 cgtcaagctg gtgatgaccc gcgaagaggt ctttcgcgcc accggcccaa cggcgtccac
     5041 gtccatcgac gtgaagatcg gcgccaagaa gaacggcgag atcaccgcag cgaccctgga
     5101 catgcgctat cagggcggcg cgtttcccgg catctggggc atgctgggat gcatgaccgg
     5161 tttcgcctgt tacgacatca agaaccagcg atccgtgggc tgggacgtca tcgtcaaccg
     5221 gccgaaggtg gccgcctacc gggcgccctc ggcacccatg gcggcgttcg cggtggaaag
     5281 caccatggac atcgtagccg gcgaggtcgg catggatgcg gtggatttcc gtctcaaaaa
     5341 cgtcgccagg gagggctcgc aggcacctta cggtccgatc ttcggtccca tcggcatcga
     5401 gtccgcgttg caggaggtga agaaacaccc gcagatgaag gcccgcctga agaagaacca
     5461 gggaaggggc gtcgcctgcg gcttctggtt caacatcggt ggtcagacct gtgtggatct
     5521 caacatcgga gaagacggca ccgtcaatct ggcagttgga accgtagacg tgggcggcgc
     5581 gcgctcgtcc ctgggaatga tcgccgcgga ggaactggga atttcctatg acgacgtcaa
     5641 ggtgcacgtg gccgacaccg catcccttgg ccacaacgac accacggaag gcagccgggg
     5701 caccttctcc tcgggcatgg cggctatatt tgccgcccgg gacgccatcg aggtgttgaa
     5761 ggatcgcgcc gcgcagatgc tggacgtgcc ccgggacgcc atcgtctggg agaagggcga
     5821 ggcgcgtgca aagggtaccc aatacagcaa cctgcccaag ctctctctca gggagatcgc
     5881 cgccaaggcc gcacgcacgg gtggccccat cgc
//
LOCUS       DBNHKV010000010         5105 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1842739, whole genome shotgun sequence.
ACCESSION   DBNHKV010000010 DBNHKV010000000
VERSION     DBNHKV010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1842739"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="AC16PP_00255"
     CDS             complement(<1..671)
                     /locus_tag="AC16PP_00255"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HIR6480081.1"
                     /translation="MEDRALNRVYTGRECRELDRIAIEEFGIPGFELMMRAGRAAFSE
                     LLARWPDASCLTILCGKGNNAGDGYVIGGLAREIGMAVQLVQLGDAADLAGDAARARD
                     WAVERGVLIEPGPERALRGDVLVDALLGTGIKGELKPAYREQVEAVNGSGISVLAVDV
                     PTGVDADTGAACEPSVRADVTVTFIARKLGLVTGPGASFCGNLVLAGLGVPDQVYDSV
                     AGTALL"
     gene            739..1827
                     /gene="queG"
                     /locus_tag="AC16PP_00260"
     CDS             739..1827
                     /gene="queG"
                     /locus_tag="AC16PP_00260"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIR6480082.1"
                     /translation="MADLAALRKDIETWAGELGFQQIGVTDVDLSAHEPRVRAWLERG
                     FQGTMSYLERNLDKRFHPEKLETETCRVIVARMDYLTDPTSPRAALENPDRAYVSRYA
                     LGRDYHKLIRGRLATLARRIDSAAAGMQGRYRAFTDSAPVLEKALAEKAGLGWIGKNT
                     LLLNSKGGSWFFLGEIYTNLPLPLDEPGEPGEDGAGCGNCNACMTVCPTDAIVAPGQL
                     DARRCISYLTIELKEAIPEPLRAPIGNRIFGCDDCQIYCPWNRFAGVTNEPDFQPRHG
                     LEAATLLDLFGWSPETFERNTRGSAIRRISWDQWRRNLAVALGNGSPSAAALGALREA
                     RAGASAMVCEHIDWAIKRLTRRSGGSEP"
     gene            complement(1832..2377)
                     /gene="orn"
                     /locus_tag="AC16PP_00265"
     CDS             complement(1832..2377)
                     /gene="orn"
                     /locus_tag="AC16PP_00265"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HIR6480083.1"
                     /translation="MAMPTNLVWMDLEMTGLDPETDVIIEMATVITDSDLNILAEGPV
                     LAVWQPQAKLDAMDEWNQTHHGESGLIDRVRASAVSEREAEARTLAFIEGHVERKQAP
                     LCGNTIWQDRRFLTRYMPTLEGYLHYRNIDVSSLKELVARWRPELLKGINKANRHTAL
                     SDIRESIEELRYYRKHFIALS"
     gene            2563..3306
                     /locus_tag="AC16PP_00270"
     CDS             2563..3306
                     /locus_tag="AC16PP_00270"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR6480084.1"
                     /translation="MVHVGDAAGYLQGHVPGAVLVEPGELIGGAPPATGRLPSRERLE
                     ALFSRIGFDPDREVIVYDDEGGGWAGRMAWTLDVVGHTRWTYLNGGIHSWLAAGLPVE
                     TGPGLPVPPANVTIDIQGEPIAELEDVMAAIEDDDQVVWDVRSREEYLGLRSGSRRAG
                     HVPGAVNVDWMHLKDPERDLRLVDDVRSLLESRGIVPGKRIITHCQTHHRSGLSYMVA
                     RLLGYPRICAYHGSWAEWGNRDDTPVSRS"
     gene            3303..4130
                     /gene="asd"
                     /locus_tag="AC16PP_00275"
     CDS             3303..4130
                     /gene="asd"
                     /locus_tag="AC16PP_00275"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937125.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HIR6480085.1"
                     /translation="MSAWFAALQRLLPQHELSRFVGALAKSEAPWIRKPFIHGFARAY
                     GVSLDEAVCQELDDFRCFNDFFTRALRQDARPLARDPAAVLCPADGTVSQTGLIRDGQ
                     LLQAKGHRYSLRSLTGEADRAFEGGTFATVYLAPSDYHRVHLPADGTLLATTAIPGAL
                     FSVNAQTETTVEGLFARNERLVCRFRTGHGQMLVVLVGALIVASIETVWAGPESPYQS
                     VTTTSYDDAFARGAEIGRFVLGSTVIVCFEPGRIELLPHIKAGATVRMGEAIGAFCD"
     gene            complement(4131..5006)
                     /locus_tag="AC16PP_00280"
     CDS             complement(4131..5006)
                     /locus_tag="AC16PP_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase GenX"
                     /protein_id="HIR6480086.1"
                     /translation="MNAPPPAWRPACSIEALGARAAMLRTIRDFFFERGVIEVQSACL
                     GAHTVTDPAVDSLAVPGIGYLQTSPEYQLKRLLAAGAPSLYQLGPVFRGGEAGRLHNP
                     EFTMLEWYRLGYTDAELMAEVAELIDSVLGAADYERLSYERVVALGGGAESGAERAEL
                     DLAFADGIAALAGRRVFVVDYPADQAILARLRPGRPNVAARFELCVDGVELANGYWEL
                     GDRAAIERRFAEDLAARRQAGSELPEPDGAFLDAMDAGLPECAGVALGVDRLLMIKLG
                     AGSLAEVMAFPADRA"
     gene            complement(5003..5101)
                     /locus_tag="AC16PP_00285"
     CDS             complement(5003..5101)
                     /locus_tag="AC16PP_00285"
                     /inference="COORDINATES: protein motif:HMM:NF020845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480087.1"
                     /translation="MSTGATIKVPLFINVGDVLRVDTRKGEYVSRA"
BASE COUNT          861 a         1752 c         1673 g          819 t
ORIGIN      
        1 aggagggcag tgccggctac cgagtcgtac acctgatcag gcacgccgag gccggcaagc
       61 accaggttgc cgcagaacga tgcacccggc ccggtgacca gccccagctt gcgagcgatg
      121 aaggtcaccg tgacgtcggc cctgaccgaa ggctcacatg ccgcgccggt gtccgcatcc
      181 acgccggtag gtacgtccac cgcgagcacg ctgatccccg agccgttgac ggcctcgacc
      241 tgctcgcgat aggccggctt cagctctccc ttgatgcccg tgcccagcag agcgtcgacc
      301 aggacatcgc cacgcagcgc tctttccggt cccggctcga tgaggacgcc acgctcgacg
      361 gcccagtctc tcgcccgcgc ggcgtcgccc gcgaggtccg cggcatcgcc cagctggacc
      421 agctgcacgg ccatgccgat ttcccgggcg agcccgccga tgacataccc gtcccccgcg
      481 ttgttgccct tgccgcacag gatcgtgagg cacgaggcat cgggccagcg ggccagcagc
      541 tcggagaagg cggcgcgccc ggcacgcatc atgagctcga agccgggaat cccgaactcc
      601 tcgatggcga tgcggtcgag ctcgcggcac tcgcggccgg tgtaaacgcg attcagggct
      661 cgatcctcca catcaccgtc gatgaagacc aggcattata ctgggtggct tccccgccga
      721 tacgtggatt cagagcggat ggccgaccta gcagcgctgc gcaaggacat cgagacgtgg
      781 gccggcgagc tcggctttca gcagatcggc gtcaccgacg tcgatctgtc ggcccacgag
      841 ccccgcgttc gagcctggct ggaaaggggc ttccagggca ccatgtcgta cctcgagcgg
      901 aatctcgaca agcgctttca cccggaaaag ctcgagaccg aaacctgtcg ggtcatcgtc
      961 gcgaggatgg attacctgac cgaccctacc agccccaggg cggctttgga gaatcccgac
     1021 cgagcctacg tctcgcgcta cgcgctgggc cgcgactacc acaagctgat ccgcggccgg
     1081 ttggcaaccc ttgcccggag aatcgactct gcggccgcgg gtatgcaagg gcggtaccgg
     1141 gccttcaccg actcagcccc cgtgctcgag aaggcactgg cggaaaaggc cgggctgggc
     1201 tggatcggca agaacaccct gctcctcaac tcgaaaggcg gatcgtggtt ttttctcggc
     1261 gagatctaca cgaacctgcc gttgccgctc gatgaacccg gcgagccggg cgaggacggc
     1321 gccggctgcg gcaactgcaa cgcctgcatg accgtgtgcc cgacggatgc catcgtcgcg
     1381 cccgggcagc tggatgcacg ccggtgcatc tcctatctca ccatagagct caaggaggcg
     1441 attcccgagc cgcttcgcgc gcccatagga aaccgcatct tcggctgcga cgactgccag
     1501 atctactgcc cgtggaaccg ctttgccggc gtcaccaacg agccggactt ccagccacgc
     1561 catgggctcg aagcggcaac gctgctggac ctgttcggct ggtccccgga gaccttcgag
     1621 aggaacacgc gcggttcggc cattcggcgc atcagctggg atcagtggcg ccgaaacctc
     1681 gccgtggcgc tgggcaacgg ctctcccagc gcggccgctc tgggggccct gcgggaagcg
     1741 cgggcgggcg catcggccat ggtttgcgaa cacatcgact gggcgatcaa aagattgacc
     1801 cgacgatcgg gcggatctga gccgtgagcc gtcaggacaa cgcgatgaag tgcttccggt
     1861 agtaacgcag ctcctcgatg gattcccgaa tgtcggacag cgcggtgtgc cgattggctt
     1921 tgttgatccc tttcagaagc tccggccgcc agcgtgccac cagctccttg aggctgctca
     1981 cgtcgatgtt gcggtaatgc aggtagccct caagggtggg catgtagcgg gtgagaaacc
     2041 gcctgtcctg ccagatggtg ttcccgcaca ggggcgcctg ctttcgctcg acgtggccct
     2101 cgatgaaggc aagggtccgc gcctctgcct cccgctcgga gaccgctgag gcccgcacgc
     2161 gatcaatgag ccccgactcg ccgtggtggg tctggttcca ctcgtccatg gcgtccagct
     2221 ttgcctgggg ttgccaaacg gcgagcaccg ggccctcggc gagaatgttg agatcgctgt
     2281 cggtgatcac cgtggccatt tcgatgatga cgtcggtttc agggtcgaga cccgtcatct
     2341 cgagatccat ccacaccagg tttgtcggca ttgccactcc taaacggatg cgggtcgccc
     2401 gcccgccgcc ggccattctg ccacaagcct gggatctgcc acggcgattc agttctcggc
     2461 taagataggc ggcccttttg ccccggacag gaagccgtca tcgcgccacc gagaatcatc
     2521 gaacccgagg agctggccgg cgccgacccg gagggccggc tcgtggtgca tgtgggcgat
     2581 gcggccggct acctgcaggg gcacgttccc ggcgccgttc tcgtcgagcc tggggagctg
     2641 attggcggcg cgccgccggc taccggccgg ctgccgagca gggagaggct ggaagcgctt
     2701 ttctcgcgaa tcggtttcga tccggaccgg gaagtcatcg tctacgacga cgagggcggc
     2761 ggctgggccg gccggatggc gtggacgctg gatgtggtgg gccacacccg ctggacctac
     2821 ttgaacggcg gcatccacag ctggctggcc gccgggctgc cggttgagac cggaccgggg
     2881 ctgcccgtgc cgccagcaaa cgtcacgatc gacattcaag gcgaacccat cgcggagctc
     2941 gaggacgtca tggctgccat cgaggacgac gatcaggtgg tctgggacgt ccgctctcgc
     3001 gaggagtatc tggggctgcg cagcggttcc cgtcgggccg gccacgttcc cggcgcggtg
     3061 aacgtcgact ggatgcacct caaggacccc gagcgcgacc tgcgcctggt ggacgacgtt
     3121 cgctccctgc tcgaatccag gggaatcgtg ccgggcaagc gcatcatcac ccactgccag
     3181 actcatcacc gctccggcct ctcctacatg gtcgcgcggc tgctcggcta cccccgaatc
     3241 tgcgcctacc acggatcctg ggccgagtgg ggcaatcgcg acgacacccc ggtgagccgg
     3301 tcgtgagcgc ctggttcgcc gcgctgcaga ggctgcttcc ccagcacgag ctgtcccggt
     3361 tcgtcggcgc cctggcgaaa tcagaagcgc cctggatccg caaacccttc attcacgggt
     3421 tcgcacgagc ctacggggtg tcgctcgacg aggcggtatg tcaggagctg gacgatttcc
     3481 gctgcttcaa cgacttcttc acccgcgcgc tacgtcaaga cgcccggccg ctggcccggg
     3541 atccggccgc cgtgctctgc ccggctgacg ggaccgtgag ccagacgggc ctgatccggg
     3601 acggtcagct gctgcaggcg aaagggcacc ggtactcgct tcgctcgctg acgggcgagg
     3661 ccgatcgggc attcgaaggc ggaacgttcg caaccgtcta tcttgcacca agcgactatc
     3721 accgcgtcca cctgccggcg gacgggaccc tgctcgccac cacggcgatt ccgggggcgc
     3781 tcttttccgt caacgcgcag acggagacca ccgtcgaggg cctgttcgcg cgcaacgagc
     3841 ggctggtctg ccgattccgc acgggccacg gccagatgct cgtcgttctg gtgggcgcgc
     3901 tcatcgttgc aagcatcgag acggtgtggg cgggccccga gtcgccctac cagagcgtca
     3961 cgacgacctc ctacgatgac gccttcgcac ggggcgcgga gatcggccgc tttgtgctgg
     4021 gctccacggt gatcgtctgc ttcgagcctg gcaggatcga gctgctgcca cacatcaagg
     4081 ccggcgcgac cgttcgcatg ggcgaagcga taggcgcttt ctgcgactga tcaggcccgg
     4141 tcggcgggaa aggccatgac ctctgccagg ctccccgcac ccagcttgat catcagcaac
     4201 cgatcaacgc ccagcgccac gccggcgcat tcgggcagcc ccgcgtccat agcgtccagg
     4261 aacgcgccgt ccggctcagg cagctcggaa cccgcttgcc tgcgcgcggc gagatcctcg
     4321 gcgaagcggc gctcgatggc ggcacgatcg ccgagctccc aatagccgtt tgcaagctcc
     4381 acgccgtcga cgcacagctc gaaacgcgcg gcaacattcg gccgcccggg gcgcagccgc
     4441 gcgaggattg cctgatctgc cggatagtcc accacgaaca cccggcggcc ggcgagggcg
     4501 gcgatcccat ccgcaaacgc gaggtccagc tctgcacgct ccgccccact ctcggcgcca
     4561 ccgcccaggg caacgacccg ttcgtagctg agcctctcgt agtcggcggc ccccagcacc
     4621 gagtcgatca gctcggcaac ctcggccatg agctccgcgt cggtatagcc aagccggtac
     4681 cactccagca ttgtgaattc ggggttgtgc aaccgtcccg cctccccgcc gcgaaatacg
     4741 ggcccgagct ggtaaaggga aggtgccccg gctgcgagca ggcgcttgag ctgatactcg
     4801 ggagatgtct gcagatagcc gatcccgggc accgccagcg agtcgacggc cgggtccgtg
     4861 acggtgtgcg cgccgaggca ggcagactgc acctcaatga ccccgcgctc gaaaaagaaa
     4921 tcccggatcg tccgcagcat ggcggcgcgc gcgccgagcg cttcaatcga gcacgccggg
     4981 cgccaggcgg ggggtggcgc gttcaagcgc ggctgacgta ctcgcctttg cgggtatcga
     5041 cgcgcagcac gtctccaacg ttgatgaaca agggcacctt gatggtcgcg ccggtagaca
     5101 gcgtg
//
LOCUS       DBNHKV010000011         6850 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1701300, whole genome shotgun sequence.
ACCESSION   DBNHKV010000011 DBNHKV010000000
VERSION     DBNHKV010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6850)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1701300"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..588)
                     /locus_tag="AC16PP_00290"
     CDS             complement(169..588)
                     /locus_tag="AC16PP_00290"
                     /inference="COORDINATES: protein motif:HMM:NF016160.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HIR6480088.1"
                     /translation="MTTDLLTCRATVVASGPDGVEVGVMSPCGGCKAGCGSASRTGAR
                     MTLSSRAAVSGAPAAGTPVELAVSRSGLTKACALVFGIPIAGWLAATALVDAYWGEAV
                     ASAAGLGMLAWLLFAVRAFRPALRRWANIELIPLSRE"
     gene            complement(592..1578)
                     /locus_tag="AC16PP_00295"
     CDS             complement(592..1578)
                     /locus_tag="AC16PP_00295"
                     /inference="COORDINATES: protein motif:HMM:NF015825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HIR6480089.1"
                     /translation="MRPARFILLVFGVLVSAISVADEDPHAWLADMNRAFSELSYDGI
                     FTYFSGDDLDTLRVVHMVVDEVQRERLVHLNGAPREIVRTGEEVACILMPGDELLELE
                     GSIPSGPFARAFVRRYDRISSSYGLSLYGEDRIAGRQAVRMAVSPLDDDRFGYRLWLD
                     KETRLLLRSELIDADGNRLEIFQFNQIVFGDAVSLDALEPAGPDGSLVSHLTLARDDK
                     TPVPESRSAWQAGWLPAGFTMAAADIRRTPSSLKSIDTMMYSDGLAAFSVFIEDMPPA
                     GAASMVSRKGATVAVTHLVANAEDEHHLVTLVGELPTDTAQRIARSITFRQD"
     gene            complement(1575..2144)
                     /locus_tag="AC16PP_00300"
     CDS             complement(1575..2144)
                     /locus_tag="AC16PP_00300"
                     /inference="COORDINATES: protein motif:HMM:NF015810.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HIR6480090.1"
                     /translation="MSEKLRESLSATMDGEADEFELRRVLDEMQKDGGLREVWSRYHI
                     AGMLMRGERVHFSSDIRERVWSQLQLGTDDEAVESAALPAAQAGPADGPRSRLGRLAG
                     LAVAASVAFAAVIGVIGLWDEGAGEPPATAGTVTIEGPRVSNPAFTSEISPRDLQRAN
                     AYMMHHFQHQGMNQPGVSSFVKMVTYERR"
     gene            complement(2268..2849)
                     /gene="rpoE"
                     /locus_tag="AC16PP_00305"
     CDS             complement(2268..2849)
                     /gene="rpoE"
                     /locus_tag="AC16PP_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HIR6480091.1"
                     /translation="MAKDTDKELVRRVQKGDRYAFDLLFSRYQHKILNLVSRYLKDPQ
                     DVEDVTQEAFIKAFRALPRFRGESAFYTWLYRIAINTAKNHLVARGRRPPGVDVDMED
                     AEIMDGADALRVSESPEAVLERDELSAAIDVAISQLPDDLRSAVTLREFDGLSYEQIA
                     HIMDCPVGTVRSRIFRARESIDAHIEPLLPRRR"
     gene            2948..4567
                     /gene="nadB"
                     /locus_tag="AC16PP_00310"
     CDS             2948..4567
                     /gene="nadB"
                     /locus_tag="AC16PP_00310"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016568828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HIR6480092.1"
                     /translation="MGQRFSTDILVIGGGAAGLSAALHLAEHARVTVLCKGDVESAST
                     HWAQGGIAAVTDPDDTYEAHIQDTLNAGAGLCDHSVVAQTVERAPQVISQLVQWGIEF
                     DRSENEYHLTREGGHSHRRILHVADATGKALTQTLTDKVLDHPNIELFTDRVAIDLIN
                     LSKFGRHPNRCAGAYVLNRTTNHVEVFQAHFVILATGGASKVYLYTSNPDSSTGDGIA
                     MAWRAGCRVANMEFNQFHPTCLFHPHAKSFLISEAVRGEGGVLCLPSGERFMERFDPR
                     LELAPRDIVARAIDHEMKRLGLDCVYLDISHRPRSFIKSHFPTIAQRCADFGIDMANE
                     PIPVVPAAHYTCGGVVIDGNGASDVPGLYAVGEVSCSGLHGANRLASNSLLECLVFGE
                     AAARDIKRRLDEPRWDVNVPQWDESQVVDSDEDVIIAHNWEELRRFMWDYVGIVRTDK
                     RLQRAQRRIDLLKQEVHEYYSNYRVTNDLIELRNLIHVAELIVRSALVRKESRGLHYN
                     LDHPETMSRADDTILFPGSMDRLDVLQPVFS"
     gene            complement(4549..4851)
                     /locus_tag="AC16PP_00315"
     CDS             complement(4549..4851)
                     /locus_tag="AC16PP_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480093.1"
                     /translation="MAGIATLAAAAGWMWLGASGLALVVLWFRARWPLPDVVRYQLDA
                     RRVRLVSLGVVRVRWTEGWFKAGEVFSDEIDPADFAALRRFLKAAVRDRTDQLKTG"
     gene            complement(4877..5134)
                     /locus_tag="AC16PP_00320"
     CDS             complement(4877..5134)
                     /locus_tag="AC16PP_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006105 - succinate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0034552 - respiratory chain complex II
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HIR6480094.1"
                     /translation="MEGDASQGRIYWRSRRGLLELELLLIPFVRERFEELPQDDQDRY
                     VRLLEHEDLDIYDWLQGRSKPEDESLHAIVDRIRRHNSSRV"
     gene            5204..6088
                     /gene="ygfZ"
                     /locus_tag="AC16PP_00325"
     CDS             5204..6088
                     /gene="ygfZ"
                     /locus_tag="AC16PP_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAF17-like 4Fe-4S cluster assembly/insertion
                     protein YgfZ"
                     /protein_id="HIR6480095.1"
                     /translation="MLRQKWLSQTHPVTGEPDAPAGVCYLPDLSVLRFEGNDAATFLQ
                     GYVTCDTQKLSPKRLQPAAVCNLQGRVVFNGWCVRADDAEKGPVDLIVHRSLAPRVAS
                     FLEAYLRFSRTSLADMGEDILVFGYLANDPPPGALRISEDIGLLLADDLEKAEKIWHD
                     LPHAPTEVWQAHRLAEGWPLISTPTSESFLPQMLDLDRLGAISFDKGCYLGQEIVARA
                     QHRGKVKRRLARLNWTGPEAPAAGTEVTHADDASRACGTLVEAARLSEHGGIAIAVIA
                     EAAPDELRVGECRLSARG"
     gene            complement(6117..6689)
                     /locus_tag="AC16PP_00330"
                     /pseudo
     CDS             complement(6117..6689)
                     /locus_tag="AC16PP_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
BASE COUNT         1319 a         2322 c         2038 g         1171 t
ORIGIN      
        1 gctctggacg gtgcttatgt tcacaacggc cggcgagttc ttggcgacca tgtccacgaa
       61 atcaggcagc tgcgccccaa agcccgcctc tacccaggca aggagcagaa tcaggtaacc
      121 aaatctaagc atatttctca tacaaccatc ggtcgcctcg cggcgccatt actcgcgtga
      181 aagcgggatt aactcaatat tggcccagcg ccgcaacgca ggacgaaacg cccgaaccgc
      241 aaacagcaac caggcgagca tgccaagccc cgccgccgag gccacggctt cgccccaata
      301 agcgtcgacc agcgccgtgg cggccagcca gcccgcgatc gggataccga aaaccagggc
      361 acaggccttc gtcagaccgc tgcgggacac ggcaagctcg accggcgtgc cggccgccgg
      421 ggcaccgctc acagcggccc gggaagacag ggtcatcctc gcgccggtcc tggacgcgct
      481 cccgcagccg gctttacagc caccgcaggg cgacatcacc cccacctcga ccccatccgg
      541 gccgctcgcc accacggtgg cacggcaggt gagcaggtcc gtcgtcatca atcagtcctg
      601 ccggaacgtg atggaccggg caattctctg cgcggtgtcc gtgggcaact cacccaccag
      661 cgtcaccagg tgatgttcgt cttccgcatt cgcaaccaga tgcgtgaccg cgaccgtggc
      721 gcccttacgg gaaaccatgc tggcggcgcc cgctggcggc atgtcctcga tgaatacgga
      781 gaatgccgcc aacccgtccg aatacatcat ggtgtcgata gatttcagag aactgggcgt
      841 gcgacggatg tccgccgcgg ccatggtgaa cccggctggc agccaccctg cctgccaggc
      901 actgcgggac tccggcaccg gcgtcttgtc gtcccgcgca agggtcaggt gggaaaccag
      961 ggagccgtcc ggtccagcag gctcgagggc gtccaggctg accgcgtcgc cgaagacgat
     1021 ctggttgaac tgaaagatct ccaggcggtt gccgtcggcg tcgatcagct cggaacgcag
     1081 cagcagccgg gtctccttgt ccagccacag gcgatagccg aagcgatcgt cgtctagcgg
     1141 ggaaacggcc atgcgaaccg cctgacgccc cgcgatgcgg tcctcgccgt agagcgagag
     1201 cccataggaa ctcgaaatgc ggtcgtagcg ccggacgaag gcgcgcgcga agggcccgga
     1261 cgggatgctg ccttcgagct ccagcagctc gtcacccggc atcaggatgc aggccacctc
     1321 ctcacccgta cgcacgatct cccgcggcgc tccgttgagg tgcacgagcc gttcccgctg
     1381 aacctcgtcg accaccatgt gcaccacccg gagggtgtcg agatcatcgc cactgaaata
     1441 ggtgaatatg ccgtcgtagc tgagctcgct gaaggcacgg ttcatatcgg ccagccacgc
     1501 gtggggatcc tcgtcggcca cggaaatggc cgaaaccaac acgccgaaca ccagcagaat
     1561 gaagcgtgca ggtctcatcg acgctcgtag gtcaccatct tcacgaacga ggagacgccg
     1621 ggctggttca tcccctgatg ctgaaagtga tgcatcatgt acgcgttggc tcgctgcaga
     1681 tcgcggggcg agatctcgct ggtaaacgca ggattgctga cccggggccc ttcgatggtc
     1741 accgtgcccg cagtcgccgg gggctcgccc gctccttcgt cccacagacc gatcacgccg
     1801 atcaccgccg caaaggcgac cgacgccgcc actgcgagcc cggccaatct gcccagtctg
     1861 gaccgcgggc catccgcggg cccggcctga gctgccggca gcgcggccga ttcaaccgct
     1921 tcgtcatcgg taccgagctg caactggctc cagacccttt cccggatatc gctgctgaaa
     1981 tgcacgcgtt cgccccgcat caacattcct gcgatgtggt agcggctcca aacctcacgc
     2041 agcccaccat ctttctgcat ctcgtcgagg acgcgacgca gttcgaactc gtcagcctcg
     2101 ccgtccatgg tcgccgaaag cgactctctc agtttttccg acatctcctg cttcccctgc
     2161 tctcgtcttc aacgagcacc ttctttaccc tatccttacc ctgtcctgcg cggtctctcc
     2221 ccgcgcagcc gtatcgacca ccaaccgctg acccagcaac ctcgccgtca ccgcctgcgc
     2281 ggcaacagtg gctcgatgtg agcatcgatg gattctctgg ccctgaatat ccttgaacgt
     2341 accgtaccca ccgggcaatc catgatgtga gcgatctgct cgtagcttag accatcgaat
     2401 tcccgaagag ttaccgcgct gcgcaggtcg tcgggcaatt gagatatcgc cacatcgatg
     2461 gcggcggaaa gctcatcccg ttccagcacc gcttccgggc tttcgctcac ccgcagggcg
     2521 tcggcaccgt ccatgatttc cgcatcttcc atgtccacgt ctactccggg cgggcgccgc
     2581 ccgcgtgcga cgaggtggtt cttcgcggtg ttgatcgcga tgcggtacag ccaggtatag
     2641 aacgcgctct cgcctcgaaa tcgcggcaag gcccgaaacg cctttatgaa agcttcctgt
     2701 gtaacgtcct ccacatcctg gggatccttg aggtagcggg aaacgaggtt gaggatcttg
     2761 tgctgatagc ggctgaacaa aagatcgaac gcgtagcggt cacccttctg gactctcctt
     2821 accagttcct tgtcggtatc ttttgccact tagacccggt tcgaccccgg tgttccccaa
     2881 gaggcccgat attctgccga ttgcgtcccg tttatactag ttggctaact gattccgggc
     2941 ggtggacatg ggccagcggt tctccacaga cattctcgtc atcggcggcg gcgcggccgg
     3001 cctgtcggcc gctctgcacc tcgccgagca tgctcgtgtt accgttctgt gcaagggtga
     3061 cgtcgagagc gcttccactc actgggctca gggcggcata gccgcggtga cggatccgga
     3121 cgacacctac gaggcccaca tacaggacac gctgaacgcc ggcgccggtc tgtgcgatca
     3181 cagcgtcgtg gcgcagacag tggagcgcgc gccccaggta atcagccagc tggtgcagtg
     3241 gggcatcgag ttcgatcgca gcgagaacga gtaccacctg acccgcgaag gtggccactc
     3301 tcatcgccgc attcttcatg tcgccgacgc caccggcaaa gcgctcaccc aaaccctcac
     3361 cgacaaggtc ctcgaccacc cgaacatcga gctcttcacg gatcgggtcg ccatcgacct
     3421 gatcaacctg agcaagttcg ggcgccaccc aaaccgctgc gccggcgcct acgtgctcaa
     3481 ccgaaccacc aaccacgtgg aggttttcca ggcccacttc gtgattctgg cgaccggcgg
     3541 cgcgagcaag gtgtatctgt atacgagcaa cccggacagc tccacgggcg acggcatcgc
     3601 catggcgtgg cgggccgggt gccgcgtggc gaacatggag ttcaaccagt tccaccccac
     3661 ctgcctgttt cacccccacg ccaaatcgtt cctcatctcc gaggcggtcc gtggggaagg
     3721 cggcgtgctg tgcctgccct ccggggagag attcatggag cgtttcgatc cccggctgga
     3781 actcgccccg cgcgacatcg tcgccagagc catcgaccac gagatgaagc ggcttgggct
     3841 ggactgcgtc tacctggaca tcagtcaccg gccgcgctcg ttcataaaga gccactttcc
     3901 taccatcgcc cagcgttgcg ccgacttcgg catcgacatg gcaaacgaac ccatccccgt
     3961 cgtgccggcc gctcactaca cctgcggggg cgtggtcatc gatggcaacg gcgccagcga
     4021 cgtgcccggg ctatacgccg tgggcgaggt gagctgctcc gggctgcacg gagcaaaccg
     4081 gcttgcgagc aactcgctgc tcgaatgcct ggttttcggg gaagccgccg cccgggacat
     4141 caagcgccgc ctcgacgagc cgcggtggga cgtcaacgtg ccgcagtggg atgagagcca
     4201 ggtggttgat tccgacgagg acgtcatcat cgcccacaac tgggaggaac tcaggcgctt
     4261 catgtgggac tacgtcggca tcgtcaggac ggacaagcgc ctgcagcgcg cgcagcggcg
     4321 gatcgacctc ctcaagcagg aggtgcacga gtactacagc aactatcgcg tcaccaacga
     4381 cctgatcgag ttgagaaatc tgatccatgt cgccgagctc atcgtgcgct cggcgctggt
     4441 ccgaaaggag tccaggggcc tgcactacaa cctcgaccat cccgagacga tgagtcgggc
     4501 ggacgacacc atcctgttcc cgggatcgat ggaccgtctg gacgtgcttc aacccgtctt
     4561 cagctgatcc gtccggtctc ggaccgcggc tttgagaaac cggcgcaggg cggcgaagtc
     4621 cgccgggtcg atctcgtcgg agaacacttc cccggccttg aaccagccct cggtccagcg
     4681 cacccgcacc acccccaggc ttacgagcct gacgcgccgc gcatcgagct ggtagcggac
     4741 gacatcgggc aacggccacc gggcccggaa ccagagaacg accagcgcca ggcccgacgc
     4801 tccaagccac atccagccgg cggcggcagc cagagtcgcg atgccggcca cctcgaggcg
     4861 gcgaagccgc ccggtttcaa actcgagaag aattgtgtct gcggattctg tccacgatgg
     4921 cgtgcaggga ttcatcttcc ggcttcgaac gcccctgaag ccagtcgtag atgtcgagat
     4981 cctcatgctc gagaagccga acgtagcggt cctgatcgtc ttgcggcagt tcctcgaatc
     5041 gttcgcgtac aaacggaatc agcagaagct cgagctccag caatccgcgc cggctgcgcc
     5101 agtaaattcg accctgagac gcatcgcctt ccattgctcg acactaaccc caatgcggtt
     5161 aacatgcacc atcgaaaccc tgacgctcag gtgccatatc agcatgctca gacaaaagtg
     5221 gctgtcgcaa actcacccgg taaccggtga gccggatgct ccggcgggcg tgtgctacct
     5281 gcccgacctc agcgtcctgc gcttcgaagg caacgatgcg gccacgttcc tccagggtta
     5341 cgtgacttgc gacacccaga agctcagccc gaagcgtctg caaccggcag cggtctgcaa
     5401 cctgcagggt cgggtggtct tcaacggctg gtgcgtccgc gccgatgacg ccgagaaagg
     5461 cccggtggat ctcatcgtgc accgatcgct tgcaccgcgc gtcgcgagct ttctggaagc
     5521 ctatctgagg ttctcccgca ccagcctggc agatatgggg gaagacatcc tggtgttcgg
     5581 ctacctcgcg aacgatccgc cacccggggc gcttcggatc agtgaggata tcggcctgct
     5641 gctggcagat gacctcgaga aggctgagaa gatctggcac gacctgccac atgcgcccac
     5701 cgaggtgtgg caagcccaca ggctcgctga aggatggccg ctcatcagca caccgaccag
     5761 cgagtcgttt ctgccgcaga tgctcgacct cgaccggctg ggcgcgatca gcttcgacaa
     5821 aggctgctac ctgggccagg aaatcgtcgc ccgggcacaa cacaggggca aggtaaaacg
     5881 ccggctcgcc cgactgaact ggacaggccc cgaagcgcct gcggcaggaa cggaggtgac
     5941 ccacgccgat gacgccagcc gcgcctgcgg caccctcgtc gaggcagcaa ggctgagcga
     6001 gcatggcggg atcgccattg ccgtgatcgc agaagccgcg cccgacgagc tgcgcgtcgg
     6061 cgagtgtcgg ctgagcgccc ggggctgacc ggcctccggg taccccgcag gaccggctac
     6121 cccttcagat cgggtttgaa aagccactga atgctggaga agtccagacc gccggcatcg
     6181 tgcagctgct ggtgcacgcg atagagattc ttggcgagcg accccatggg cacggcgctg
     6241 cgcgactgct ccgcggtgtc catggctagc cccagatcct ttgtcatgag atcgaccagg
     6301 aaaccgccct cgtagtcgcg ggacgcgggc gcgccctcca tgacaccagg gtaaggattg
     6361 tagacgttca gcgcccagtt accgccggag gagcgtttca tcacctcgga cagaacggca
     6421 ggatcaagac cgttgctgac gccgagcgcc agagcctccg ccgttcccgc catcagcacg
     6481 gccagcagca tgttgttgca gatcttcgcg atctggcctg caccgctggc tccggcgtgg
     6541 aatatgttac tgcccatgac attgagcacc tcgcgggcgc gctcgagggc atctgcctcg
     6601 cccccgacga tgaacgagag cgtcccggct tccgcgccgg cggtgccgcc ggataccgga
     6661 gcatcgatca tcgcgagccc ctttttttta aacctcgtgt taactgttca gtttacgcgg
     6721 acaccccaag gagctcatca tgagccagtt tgatttgaaa gcgaccctcc gacagatcgg
     6781 cccacccctg cgcaagcaac tgcttgaaga cttgcccctg ctgcaagact tcgactgggc
     6841 acaggtcaag
//
LOCUS       DBNHKV010000012         2200 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_851409, whole genome shotgun sequence.
ACCESSION   DBNHKV010000012 DBNHKV010000000
VERSION     DBNHKV010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_851409"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /locus_tag="AC16PP_00335"
     CDS             complement(<1..1320)
                     /locus_tag="AC16PP_00335"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6480096.1"
                     /translation="MITERANPSLRLFAAVAMLAALTACAAKGPYVLELMPAPTVYQD
                     EDFSPFADDFSPESLPYRGMLYVTDRRPAGAGKPPDPADPHYVGKRGEGLRAGVASIQ
                     LGEGGFTWEEAIRVSMASDRGEGYRLSVTGVEELGFLEPSIEPFIEAPDFPLADEAFA
                     ALVNDKLAQSEQKDVYIYVHGYRVRFEYPVLVATELWHFLGYEGAFIAFSWPSTPSYL
                     AYFADAEVAVVSARQLRILLEFLAQSTDVRRIHILGYSQGTRTVMQALHMLALKYEED
                     DRESLLERLRIGNVALVGSDIDRETMGMYVADGLMRVTERMTVYASAKDKALRISRRV
                     LRRDRIGQLIPSDEITDELRGFLITTPEFEIINVTDAEDATTENGHRYFRSSPWVSSD
                     LLTSFRYGMTPGQRGLEILPETPIWQFPADYPTRLRSALNDVVDDQAS"
     gene            1503..>2200
                     /locus_tag="AC16PP_00340"
     CDS             1503..>2200
                     /locus_tag="AC16PP_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480097.1"
                     /translation="MGRYSVIFLMAALLMLGSSGLRAEQGDETWWSITPYIWAMDTKL
                     DLSWRDSPVGGTEIDFKDALGNLDAAFQVAVEGGRGRWSAFADVTFVKISDATGEDAL
                     RLKTKNKQVFLDAGVAFWPGGAGSPLSIYGGARYTGFDNTYRLQFQGDDVARRDSSAD
                     YFDALLGVRYRFDLSDRWSILTLGDVSFGESEGSWQAQALAAYTVGKRRMNRIMFGYR
                     YRDTEFDDAELTTE"
BASE COUNT          390 a          659 c          728 g          423 t
ORIGIN      
        1 cgatgcctgg tcgtcgacaa cgtcgttgag ggcagagcgc aggcgcgtcg ggtaatcggc
       61 cggaaactgc cagatcggag tctccggcag gatctcgaga ccgcgttgcc ctggcgtcat
      121 tccgtagcgg aagctggtga gcagatcgct gctcacccac gggctgcttc gaaagtaacg
      181 gtggccgttc tcggtggtgg cgtcttcggc atcggtgacg ttgatgatct cgaattccgg
      241 tgtggtaatg aggaagcccc gaagctcgtc ggtgatctcg tccgagggaa tgagctgccc
      301 gatccgatcg cgccgcaata cgcgccgcga gatgcgcaga gccttgtctt tggctgacgc
      361 gtagacggtc atacgctccg tcacccgcat caggccgtcg gctacgtaca tccccattgt
      421 ctcccgatcg atgtcgctgc cgacgagggc cacgttgccg atacgcagcc gctcgagcag
      481 cgactcgcga tcgtcctctt cgtacttcag cgccagcatg tgcagggcct gcatgaccgt
      541 gcgggtgccc tggctgtagc ccagaatgtg gatccgcctc acgtcggtgc tctgcgcaag
      601 gaattccagc aggatgcgca gctgtcgggc ggagaccacc gccacctcgg cgtcggcgaa
      661 gtaggcgaga tagctcggcg tggaaggcca cgagaaagcg atgaatgccc cttcgtagcc
      721 caggaagtgc cagagctccg tggccacgag caccgggtat tcgaaccgca cccggtagcc
      781 gtgcacgtag atgtagacgt ccttctgctc ggactgggcg agcttgtcgt tgaccagcgc
      841 cgcgaacgct tcgtccgcca gcgggaaatc gggcgcttcg atgaatggct cgatgctcgg
      901 ctcgaggaac ccgagctctt ccacgccggt cacgctcaac cggtatccct cgccgcgatc
      961 gctcgccatc gacacccgaa tggcttcttc ccaggtgaag ccgccctcgc cgagctggat
     1021 ggaggcgacg cctgcccgaa ggccctcgcc gcgcttgccg acgtagtgcg ggtctgcggg
     1081 atcgggcggc tttccggcac cagccggcct gcggtccgtt acgtacagca tgccgcgata
     1141 cggcaacgat tccgggctga agtcgtcggc gaaggggctg aagtcctcgt cctggtaaac
     1201 ggtgggcgcg ggcatcagct ccagcacgta gggccctttt gcagcgcatg cggtcagagc
     1261 agccaacatg gccacggcag caaacaagcg cagcgatggg ttggctcgct cagtaatcaa
     1321 ggcttggcga cctcttgatg gctgacgttt tcattctcga agaggtcaat gatgccacat
     1381 cagcacaggt ccagtatgat gcgcgtcgtc gagggcctgc tgatttcagg tacgtggcac
     1441 gttgttggga ctagccgagc gtagcgagag gacttgcagt tatgaatctt ggatgggtca
     1501 gcgtgggacg gtattcggtc atcttcctga tggccgcatt gctgatgctt ggcagcagcg
     1561 ggcttcgcgc ggaacaaggc gacgagacct ggtggtccat cacgccctac atctgggcca
     1621 tggatacgaa gctggatctc agctggcggg actcgcccgt cggcggcacc gagatcgatt
     1681 tcaaggacgc gctgggcaat ctcgatgcgg cgttccaggt ggccgtggaa ggggggcggg
     1741 gtcgctggag cgcctttgcg gatgtgacct tcgtcaagat ctcggacgcg actggtgagg
     1801 atgcgttgcg gctgaaaacc aagaacaagc aggtgttcct ggacgccggg gttgccttct
     1861 ggccgggcgg cgccggctcg ccactgagca tctatggcgg agcgcgctac accgggttcg
     1921 acaacaccta ccgtctgcaa ttccagggcg acgatgtcgc ccgtcgagat tcgtcggccg
     1981 actacttcga tgctcttctg ggcgttcgct accgtttcga tctgtcggat cggtggtcca
     2041 tcctcaccct cggggacgta agcttcggcg aatccgaagg cagctggcag gctcaggcac
     2101 tggccgccta cacggtgggc aagcggcgca tgaaccggat catgttcggg tatcgctatc
     2161 gggacaccga gttcgacgat gccgagctga ccacggagta
//
LOCUS       DBNHKV010000013         8698 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_993742, whole genome shotgun sequence.
ACCESSION   DBNHKV010000013 DBNHKV010000000
VERSION     DBNHKV010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8698)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8698)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8698
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_993742"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(473..1870)
                     /locus_tag="AC16PP_00345"
     CDS             complement(473..1870)
                     /locus_tag="AC16PP_00345"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006720 - isoprenoid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6480098.1"
                     /translation="MAETTRPATEPTAESDYRFLSGNFAPVAEEVTAFDLPVEGELPA
                     ELNGRYLRNGPNPFDDPDPGSYHWFTGAGMVHGIRLEDGRAEWYRNRYVGSEQLAASR
                     GEPDIPGANWNDNPSGPNTNVGGFAGTTWALVEAGGCPVELTYELETVARNDFFGTLP
                     GAFTAHPKVDPQTGELHGMAYAWPQWMDHVQYVAVAPDGRVRRTVDVKLPGMTMMHDF
                     SLTQRYVVIYDQPVTVDFAMVGNTSFPMRWNPDYGNRLGLLPREGDADEIIWIDAPLG
                     YCFHPMNAYDDPDGSVIVDLCVYERMFDAYPRDPFGDSMASLQRWRVNPANRTISVDV
                     IDASANEFPRHRGSLSTLPYRYGYCASPSSDMSAGWPTLKHDLQTGERSVFDHGPGRA
                     AGEPVFVAREGSAAEDDGYLITYVHDLAQSSAEFVVMDAQDFDRGYLARVALPQRVPF
                     GFHGNWVSDRAVSPG"
     gene            2060..2932
                     /locus_tag="AC16PP_00350"
     CDS             2060..2932
                     /locus_tag="AC16PP_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480099.1"
                     /translation="MYKVFAFLKRNPNLTHDEYRAGHVGYHCGQSRRLKNIRGYLVNV
                     WDNQPLAEKIGDLAGEIAFNQPDGFLDYWDGFPQVYFDDREAWVNARTVEPNRATADG
                     LVVDPDWSLGDSPHLFMPVPGEPTQFQAHHVHMEEHVVVAVRRPEFRLTKLIQFFQKR
                     EGLTDAEFADRLLGEYAPLAGSMPGLWGYVANLRDADPEAAMRGFFAPDSWVFGEEGR
                     ALHGRFCSLWRGANELHFDSVEAFAAARRDDRVRDRLLELERELFSSMWHVEVDENLI
                     VIPNRGPAPDFYFR"
     gene            2943..3794
                     /locus_tag="AC16PP_00355"
     CDS             2943..3794
                     /locus_tag="AC16PP_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480100.1"
                     /translation="MLKVFGFVRRHPNLTHDEYRAGHVGYHNSFGRRLNNIRGYLLNV
                     RANATIEETLGTDLVQSITLDEPPGFDDLWDGWGQLLFDSLDDYLAARSPARDMAGPD
                     GLQMDPGVAGVGGDFEYLYGGSPFQFHLAENVVVPVVRPERKLFKLVQFVKRPDGLEP
                     ELFRAYLSGRYARAAATMGGLCGLVVNFRTGVDVMTHFFAPDAEGFTPEGTAIRERFF
                     SYWDAMIEYWLESPADFVAGRATGSAAENMAALEKTLMSRSFFREVDETVAVLPNRAP
                     PPPFYHR"
     gene            complement(3834..4811)
                     /locus_tag="AC16PP_00360"
     CDS             complement(3834..4811)
                     /locus_tag="AC16PP_00360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6480101.1"
                     /translation="MKFGVLQFFSWPGRRGELRQVYERGLERVRIMDANGYDAVWIAE
                     HHFSTYSVCPSIHMMGAHFAANTKRLRIGTGVSLAAFYNPLRLAEEVALLDVLSGGRV
                     NWGAGSGFDPTEFRAFGIERGDRYPQFRENVEIVLKAWRSDRLTYHGRFHCYEDIEVL
                     PKPLQDPIPVWMAASSVDSVRWAAQEGFSILMDPHSSMPDIAAKQQIYHETLIAHGHD
                     PEGRDLPVARLLAIAPTDAQAREVAEAGAQWTTGSYAKQPLALKKKDNEQDPVQRYID
                     EIILWGSPERVADRLLEQQEDGHLNYLLCAPLSNQSFRLFTDEVLPRIA"
     gene            complement(4869..6455)
                     /locus_tag="AC16PP_00365"
     CDS             complement(4869..6455)
                     /locus_tag="AC16PP_00365"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     sulfotransferase family protein"
                     /protein_id="HIR6480102.1"
                     /translation="MQDSVAELRKEAARALNQRAYERAAVLCRRLLQQDPKCADGWFL
                     MGMVAAANGRITKALELVGNAALLDADNAEYLAQKARLHLLLSQPEAARETADRALAA
                     RPEDPLTLDTLGVVYTRTGNYDQARPLLQRAVAAADDNPQFHFNLASVEQFLGNLEAA
                     AAGYERSIELHPRFFRARWALSELRKSDSDARGIDVLEHLLADSQLTAEDELYLAHAL
                     ATEYEKDARHDDAFRVLARAKARRRARIGYAHENDARLFTAIRDAFPVGSLPARAQGD
                     AGSDAIFVIGMPRTGTTLVERILDSHPEVQSLGEIQDFAMAVKTASGSRSGLILDEDV
                     ISRARDADALQIGQSYLQSIAARLGHDGRFVDKMPLNFLYAGFILSTLPGARIVCLKR
                     HPLDTCISSFRQLFALDFSYYNYHYDLQDTARYYALFRQLMGHWQACGHGRIHEVSYE
                     ALTADPESEVRHLLSYLELPWHPACLEFHRNRGAVATASTVQVRQPLYRSSVSRWKRY
                     EAHLQDAIAVLEQNGIEWRD"
     gene            complement(6523..>8698)
                     /locus_tag="AC16PP_00370"
     CDS             complement(6523..>8698)
                     /locus_tag="AC16PP_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6480103.1"
                     /translation="DEQPDILVRGLNTTGLGPGFISDTVAVYFGEIPLNVDFKPVDME
                     RVEVLIGPQGTLYGQGTLGGAIRYIPKRADTSALSIDARAKAMSNKESDGLSTDIGVT
                     LNIPLVEDVLAARVNFDRLNDKGFIDYPFVVQESGVSNPEPDFSDPADVAANLREVED
                     ANGEEATSIRANLRFTPTDGIDVNLWYLNQENETEGRQIANRLAIGTGKYESGLRYEE
                     PNDYENELWAIEATADLRFAEATLIYGETQYDELGQRDQTDLLLNFEYGYEFFPTFSS
                     FTREILEEESDTLEVRLTSQHGGPLSWVVGYFKNEVSSVGTSEEFTPGFDQFAVDNFG
                     GVQLRPDSLEYYSIDDTEEEETAFYGEVSFAITDALDVTVGYRNYEFKADSTSGFALP
                     LLDTVFFGDDPDAINVDLGDPNQGKDDGDLFKFNVSYDIENFGLLYATFSEGYRNGGV
                     NSVPLCTPEQIASDQQQLCALEDEVLIDPDTIDNYEIGLKGMFLDNTLSLNLAAFFID
                     WKDLQVAEVTANGNLPITGNGSAAESKGIEMSGRWLINDNWEVMMSYAYTNAELTETA
                     PGLVGPLDALKGTRLPGHAEHQGSFNVVYNRTVWGDVDMTLNYGLVFSTDVFNIPGGD
                     EDTLVDEDGVPGDRGGEAIPGYDVHHLSASFSRDSWTVQAFVDNLWNEYYITGTRTSR
                     RFLQDENLGPGNTINGFTLRSYGQYVGMPRNIGISVSYTFER"
BASE COUNT         1421 a         2755 c         2911 g         1611 t
ORIGIN      
        1 cctcaccgcc atcgtgctcg gggcccgcca tctcgggaat ctcattcagg acgtgctgga
       61 tctgtcgagg atagaaagcg atcaccgtga tcgatacggg gataggcgtc ccagaggcat
      121 tcagagacgg cctctttgag cagtttgcgc gggctgacgc cacgatgacc agacgctacg
      181 gtgcgccggt ccggggcttt tcatcagcca gcggctggct tcgctgctgg gaggcagggt
      241 cgaatacgcg ccgggggaga ccggcagtgt tttccgtctg gtgctggcga tctgcagggc
      301 gacgtccttc gccacaagat gggttctgca gagatgaatc cgttcacgga catgctcccg
      361 aagcccatcg acattcagcg attgcagtcg ctgctgacgc gattgctgag atctcgcgac
      421 gccgtcaaag ctgccgatcc ctgaccgggc tcgaaatccc caggagctgc cgtcaaccgg
      481 gcgatacggc gcgatcgctc acccagttgc cgtgaaagcc gaagggcacc cgctgcggca
      541 gcgccacccg ggccagatag ccgcgatcga agtcctgggc gtccatcacc acgaactccg
      601 ccgagctttg tgcgagatcg tggacgtagg tgatcaggta gccgtcgtcc tctgccgccg
      661 agccttcccg ggcgacgaac acgggctcgc cggccgcccg gccgggcccg tgatcgaaga
      721 cgctgcgctc gcccgtttgc agatcgtgct tgagcgtcgg ccagccggcg gacatgtcgg
      781 aagacggcga tgcgcagtag ccgtagcgat agggcagggt cgaaaggctg ccgcggtggc
      841 gcggaaactc gttggctgag gcgtctatca cgtcgacgct gatcgtccgg ttggcgggat
      901 tcacgcgcca gcgctgaaga gaggccatgc tgtcgccgaa ggggtcgcga gggtacgcgt
      961 cgaacatacg ctcgtagacg cagaggtcga cgatcaccga gccgtcgggg tcatcgtagg
     1021 cgttcatcgg gtggaagcag tagccgagcg gcgcgtcgat ccagatgatc tcgtcggcgt
     1081 cgccttctct cggcagcagg cccaggcggt taccgtagtc cggattccag cgcatgggga
     1141 agctcgtgtt gcccaccatg gcgaagtcca cggtcacggg ctggtcgtag atgacgacgt
     1201 aacgctgggt gagcgagaag tcgtgcatca tcgtcatgcc gggcagctta acgtccaccg
     1261 tgcggcggac ccgcccgtcg ggcgccacgg cgacgtactg cacgtggtcc atccactggg
     1321 gccaggcgta ggccatgccg tgcagctcgc cggtctgggg gtctaccttc gggtgggccg
     1381 tgaacgcgcc gggcagggta ccgaaaaagt cgttgcgcgc cacggtttcc agctcgtagg
     1441 tgagctccac cgggcagccg ccggcctcga ccagcgccca ggtggtaccc gcgaagccgc
     1501 cgacgttggt gttgggcccg gaggggttgt cgttccagtt cgctccgggg atgtcgggct
     1561 cgccgcgaga cgcggcaagc tgctcagagc ccacatagcg gttgcggtac cactccgcgc
     1621 ggccgtcctc aaggcgaatg ccgtgcacca tgcccgcgcc ggtgaaccag tgatagctgc
     1681 ccggatcggg gtcatcgaag gggttcgggc cgttgcgcag gtagcggccg ttgagctcgg
     1741 ccggcagctc cccctccaca gggagatcga acgcggtgac ctcctcggcc accggggcga
     1801 agttcccgct gagaaatcga tagtcgcttt cggctgtggg ttccgttgcg ggccgggtcg
     1861 tttctgccat ggggtgttcc tgcgatggtg gggccgcgga cgttggaaga ccggtgcggc
     1921 gtcaggccga atcatagcgc gtcccgccgg cccgcgggac acggtgccgg cgcttctcct
     1981 gccccgttgg caatgaagcc ggctgagctt cgtgtatgct ccgggttgag cgagcggttg
     2041 ccggggagct gccggatcca tgtacaaagt cttcgcgttc ttgaaacgca atcccaacct
     2101 gactcacgac gagtaccgcg ccgggcacgt gggctatcac tgcggtcagt cgcggcggct
     2161 gaagaacatc cgcggctacc tggtgaacgt gtgggacaac cagcccctgg cggagaagat
     2221 cggtgatctg gccggggaga tcgccttcaa ccagccggac ggttttctcg attactggga
     2281 cgggttcccc caggtctact tcgacgaccg ggaagcgtgg gtgaacgccc gcaccgtgga
     2341 gcccaaccga gccacggccg atgggctggt cgtggacccg gactggagcc tgggcgacag
     2401 cccgcatctg ttcatgcccg tgccgggcga gcccacgcag ttccaggccc atcacgtgca
     2461 catggaggag cacgtcgtgg ttgctgtgcg gcggccggag ttccggctca ccaagctgat
     2521 ccagttcttt cagaaacgcg aagggctcac cgatgccgag tttgccgaca ggcttctcgg
     2581 cgagtatgcg cccctggccg gcagcatgcc gggcttgtgg ggctacgtgg cgaacctgcg
     2641 cgatgcggac cccgaggctg cgatgcgcgg cttcttcgct ccggacagct gggtgttcgg
     2701 cgaggagggc agggcactgc acggccgttt ctgttccctg tggcgcggcg cgaacgagct
     2761 gcacttcgac tccgttgaag cctttgccgc cgcccgccgc gacgatcggg tgcgagaccg
     2821 tctgctcgag ctcgagcgtg agcttttctc ttcaatgtgg cacgtggagg tggacgagaa
     2881 cctcatcgtc ataccgaatc gcgggcccgc ccccgatttt tatttccgct gagggggatc
     2941 gaatgctcaa ggtttttgga tttgtccggc gccaccccaa cctcacccac gacgagtacc
     3001 gtgcgggaca cgtcggttac cacaactcct tcggccgccg gctcaacaac atccgcggct
     3061 acctcctgaa cgtgcgcgcc aacgccacca tcgaggagac gctcggcacc gacctggtgc
     3121 aaagcatcac cctggacgag ccgcccggct tcgacgatct gtgggacggc tggggccagc
     3181 tgctgttcga ctccctcgac gactacctgg ccgcccgttc tcccgcccgg gacatggccg
     3241 gcccggacgg cctgcagatg gacccgggcg tggccggcgt cggcggggac ttcgagtatc
     3301 tctacggcgg ctcgccgttt cagtttcacc tggccgagaa cgtggtggtc ccggtggtca
     3361 ggcccgagcg caagctgttc aagctcgtgc agttcgtaaa gcgccccgac gggctggagc
     3421 ccgagctgtt tcgcgcctac ctttcggggc gctacgcgcg ggcggcggcg accatgggcg
     3481 gcctctgcgg gctcgtcgtg aacttccgga cgggcgtcga cgtgatgacc catttcttcg
     3541 cgcccgatgc cgaagggttc accccggagg gcacggccat ccgcgagcgt ttcttttctt
     3601 actgggacgc catgatcgag tactggctgg agagcccggc cgacttcgtt gccggccgcg
     3661 caacgggcag cgcggcggag aacatggccg cgctcgaaaa gacgctgatg agccgcagct
     3721 ttttccggga agtggacgaa accgtggccg tgctgccgaa tcgggcaccg ccgccgccct
     3781 tctaccatcg ctgattcgtc gctccgtgcg cttgccgagc gcgcgcctgc cgatcaggcg
     3841 atgcggggca ggacctcgtc ggtgaagagc cggaacgact ggttgctcag cggcgcgcag
     3901 agcagatagt tcaggtgccc gtcctcttgc tgctccagca gccgatcggc cacccgctcg
     3961 gggctgcccc agaggatgat ctcgtcgatg tagcgctgca ccgggtcctg ctcgttgtcc
     4021 tttttcttca gggccagcgg ctgcttggcg tagctgcccg tggtccactg agcgcctgcc
     4081 tcggccacct cgcgggcctg ggcgtccgtc ggcgcgatgg cgagcagccg cgccaccggc
     4141 aggtcgcggc cctccggatc gtggccgtgg gcgatcagcg tctcgtggta gatctgctgc
     4201 ttggcggcga tgtcgggcat ggacgagtgc ggatccatga gaatggaaaa tccctcctgc
     4261 gcggcccagc gaacgctgtc gacggaagaa gccgccatcc agaccgggat cgggtcctgg
     4321 agcggcttgg gcagcacctc gatgtcctcg tagcagtgga agcggccgtg gtaggtgagc
     4381 ctgtccgagc gccaggcctt gagaacgatc tcgacgttct cccggaactg ggggtagcgg
     4441 tcgccccgct cgattccgaa ggcgcgaaac tccgtgggat cgaagccgct gcctgctccc
     4501 cagttgaccc ggccgccgga gagcacgtcc agcagcgcca cctcctcggc cagccgcagc
     4561 gggttgtaga aggcggccag ggaaacgccg gtgccgatgc gcaggcgttt ggtgttggcc
     4621 gcgaagtggg cgcccatcat gtgaatggac gggcagacgc tgtaggtgct gaagtggtgc
     4681 tcggcgatcc acacggcgtc gtagccgttg gcgtccatga tgcgcacgcg ctcaaggcct
     4741 cgttcgtaga cctggcgcag ctcgccgcgg cggccgggcc agctgaagaa ctgcagaact
     4801 ccgaatttca ttcgtgcgcc gacacctgcg atgtttcggt gaagcctatc acgcagaacc
     4861 gagatctctc agtcgcgcca ctcgatgccg ttctgctcta aaaccgcaat cgcgtcctgc
     4921 agatgcgctt cgtagcgttt ccatcgcgat accgaggacc ggtacagggg ctgcctcacc
     4981 tgcaccgtgc tggcggtggc cacggcgccg cgattgcgat gaaactccag gcatgccggg
     5041 tgccagggaa gctcgaggta gctgagcaaa tgccgcacct cggattcggg atcggcagtc
     5101 agggcctcgt aggagacctc gtggatgcgt ccgtggccgc aggcctgcca gtgccccatc
     5161 agctgtcgga acagcgcgta gtagcgggcc gtgtcctgca gatcgtagtg gtagttgtag
     5221 taggagaaat ccagggcgaa aagctgccgg aagctgctga tgcaggtgtc cagcggatgc
     5281 cgtttcagac agacgatccg ggcgccgggg agggtgctga gaatgaagcc ggcatagaga
     5341 aaattcaggg gcatcttgtc gacgaacctg ccgtcgtgtc ccagtcgggc cgcgatggac
     5401 tgcagataac tttgaccgat ctgcaaagcg tccgcgtcgc gggctcgcga tatgacatct
     5461 tcgtccagga tcaggccgct gcgtgagccc gacgcggtct ttaccgccat ggcgaagtcc
     5521 tgaatctcgc caagcgattg aacctcggga tgactgtcca gaatgcgctc gacgagcgtc
     5581 gtgcccgttc gcggcatccc gatcacgaag atggcatcgc tgcctgcatc tccctgcgcc
     5641 cgtgcgggca gcgaaccgac ggggaaggcg tctcttatcg cagtgaacag ccgcgcatca
     5701 ttctcatggg cgtatccgat gcgggcgcgc cgcctggctt tggcccgagc cagcacccgg
     5761 aacgcgtcgt cgtggcgcgc gtccttttcg tactccgtcg caagggcgtg ggccagatag
     5821 agctcgtcct cggccgtgag ctgcgaatcg gcgagcaggt gctcgagcac gtcgatgcca
     5881 cgggcatccg aatccgactt cctcagctcc gacagggccc agcgcgcccg gaagaagcgc
     5941 ggatggagct caatgctgcg ctcgtacccg gcggccgcgg cttccaggtt gccgagaaac
     6001 tgctccacgg acgcaagatt gaagtggaac tggggattgt cgtctgcggc ggccaccgcg
     6061 cgctgcagca acggccgggc ttggtcgtag ttacccgtgc gcgtgtagac cacgcccagc
     6121 gtgtccaggg tgagcgggtc ctccggcctg gcggcgagcg ctcggtctgc cgtctcccgg
     6181 gccgcctccg gctgactcag caggaggtgc agcctcgcct tctgggccag gtactccgca
     6241 ttgtcggcgt ccagcagcgc ggcgttgccg acgagctcca gggccttggt gatccgcccg
     6301 ttggcggccg ccaccatgcc catcaggaac catccgtcgg cgcactttgg gtcctgctgc
     6361 agaagacgcc ggcacagcac ggcggctcgc tcgtaggccc gctggttcag cgctctggca
     6421 gcttctttcc tgagctcggc aactgaatct tgcatccgcg caccttaggc cggccgcggc
     6481 aacgatacaa cgctgctgct ggccaggccg gtaagggcgg gctcaacgct cgaaggtgta
     6541 gctcacgctg atcccgatgt tgcgcggcat gcccacgtac tggccgtacg agcgcagcgt
     6601 gaagccgttg atcgtgttgc cgggtccgag attctcgtcc tgcaggaacc ggcgcgaggt
     6661 gcgggtgccc gtgatgtagt actcgttcca cagattgtcg acgaatgcct ggacggtcca
     6721 ggaatcgcgg ctgaacgatg ccgacaggtg atgcacgtcg taacccggga tagcctcgcc
     6781 gccccggtct cccggcacgc cgtcctcgtc caccagcgtg tcctcgtcgc cgccgggaat
     6841 attgaagacg tctgtggaga agacgagccc gtagttcagc gtcatgtcca cgtcgcccca
     6901 caccgtccgg ttgtagacga cgttgaagga tccctgatgc tcggcgtggc cgggcagacg
     6961 cgtgcccttc aacgcgtcca gagggcccac caggccgggc gccgtctccg tcagctcggc
     7021 gttggtgtag gcgtagctca tcatcacttc ccagttgtcg ttgatgagcc agcggcccga
     7081 catctcgatg cccttgctct cagcggcgct gccgttaccg gtgatgggca ggttgccgtt
     7141 ggcggtgacc tcggcaacct ggaggtcctt ccagtcgatg aagaaggcgg ccaggttcag
     7201 cgacagggtg ttgtcgagga acatgccctt gaggccgatc tcgtagttgt cgatggtatc
     7261 gggatcgatg agaacctcgt cctcgagcgc gcacagctgc tgctgatcgc tggcgatttg
     7321 ttccggggtg cacaaaggta cggagttcac gccgccattg cggtagccct ccgagaacgt
     7381 ggcgtagagc agcccgaagt tctcaatatc gtaggagacg ttgaacttga agagatcacc
     7441 gtcgtccttc ccttgattgg gatcgcccag gtcgacgttg atggcatccg gatcgtcccc
     7501 aaagaacacc gtgtccagca gcggcagcgc aaatccgctg gtgctgtcgg ccttgaactc
     7561 gtaattgcgg tagccgacgg tcacgtccag cgcatccgtg atcgcgaacg agacctcacc
     7621 gtaaaaggcg gtctcttctt cttccgtgtc gtcaatcgag tagtactcga ggctgtccgg
     7681 ccgcagctgc acgccgccga agttgtccac ggcgaactgg tcgaagccag gggtgaactc
     7741 ttcgctcgtt ccgacactcg acacctcgtt cttgaagtag cccaccaccc atgacagggg
     7801 gccgccatgc tgggaggtga gccgcacttc cagggtgtcg ctttcctctt ccaaaatctc
     7861 ccgggtgaag gaagagaagg tggggaagaa ctcgtagccg tactcgaaat taagcagcag
     7921 gtccgtctga tcgcgctgcc cgagctcgtc gtactgggtt tccccgtaga tgagggttgc
     7981 ttcggcgaat cgcaggtcgg cggtcgcttc gatggcccac agctcgttct cgtagtcgtt
     8041 gggctcctcg tagcgcagtc ccgattcgta cttgccggta ccgatcgcca gacggttggc
     8101 gatctgccgt ccctccgtct cgttctcctg gttcaggtac cagaggttca cgtcgatccc
     8161 gtcggtgggg gtgaatcgca ggttggcgcg aatggaggtg gcttcctcac cgtttgcgtc
     8221 ctccacctca cgcaggttgg ctgcgacgtc cgccgggtcg ctgaagtcgg gctccgggtt
     8281 cgacacgccc gattcctgaa cgacgaacgg gtagtcgatg aagcccttgt cgttgagccg
     8341 gtcgaagttg acgcgtgccg ccagcacgtc ctccaccagc gggatgttca acgtcacgcc
     8401 gatgtcggtg ctcaggccgt cgctctcctt gttggacatc gccttggcgc gggcgtcgat
     8461 gctcagcgcg gaggtatccg cccgcttggg tatgtagcga atggcgccgc cgagggtgcc
     8521 ctggccgtac agcgtaccct ggggaccgat caggacctcg acccgctcca tgtcgacggg
     8581 cttgaagtcg acgttcagcg ggatctcgcc gaagtagacg gccacggtgt cgctgatgaa
     8641 tccgggcccc agccccgtgg tgttcaggcc gcgcaccaga atgtccggct gttcgtcc
//
LOCUS       DBNHKV010000014         6403 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1843702, whole genome shotgun sequence.
ACCESSION   DBNHKV010000014 DBNHKV010000000
VERSION     DBNHKV010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1843702"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="AC16PP_00375"
     CDS             <1..1177
                     /locus_tag="AC16PP_00375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6480104.1"
                     /translation="HLCRRGYRRRPAHMDDRVMKIIYVVNEGRFFLSHRLALAVEAKR
                     HEVEVVVVCGANTGEQGLADAGLRYVTVPMSRSGFNPFKELRTAAALLRIYRAEKPDL
                     VHHVTIKPVLYGTSMARWSGVPAVVNAVPGMGFVFTRRGLLARIRRSVVQLLYRLCMF
                     HPNMRVIFQNNEDMQAFTQRGIVDRQHVALIRGSGVDLDVFRCAPEPPGPVTFVLVGR
                     MLRHKGVYEYVEAARRVRQRRPDWRFQLVGDVDPGNPASLRPDELIAWQQAGEIEWLG
                     QRRDVPEVLAAAHVVCLPSYREGLPKTLLEAAACGRAMIASDIAGCREVVRPNVTGLL
                     VPPRVSEPLADAMLRLGEDDALRHRFARAARSAAEEVFSLEDVVRHTFRVYDELLLP"
     gene            1174..2103
                     /locus_tag="AC16PP_00380"
     CDS             1174..2103
                     /locus_tag="AC16PP_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6480105.1"
                     /translation="MRRAVVTGAGGFIGTHLCRALRHEGWEVAVITRAHALPGCERLP
                     VTLASADVELSRALGGAQVVFHLAGLAHGRASGAAEDELHRVNVESSARLLAASEAAG
                     VPAMIWLSSIKVLGDTSERPLRPEDAYRAGDAYARSKVEGERALLDREGFTRAAIVRP
                     PLVYGPGVAGNFASLARWVGSGVPLPLGAASAPRSMVGVDNLCSLMLRLAEEGSGVFH
                     VADARDRSVAQLIRAMAVLLGVPDRSWRVSPRLLAAVASALGQRARYTRLFEPLQVDQ
                     SMTRERLGWQPPHDPDQQLKETMRWFRERRQAT"
     gene            2073..3056
                     /locus_tag="AC16PP_00385"
     CDS             2073..3056
                     /locus_tag="AC16PP_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIR6480106.1"
                     /translation="MVSGTATGYLIAGFAASTVLVFLMLRVGRALNLMAVPNARSSHV
                     VPTPTAGGLGFVIPLLVFFGMLYLDGVDVALGLLLSLVLVAAASLWDDFAELSAGLRF
                     TCQTAAVALIVWQLDLPWAWYLLGLTGLALLWHVNLFNFMDGIDGIAAGQCLLYVVGV
                     QVLTLGAPGWMGELCWLTAGAMLGFLAYNWPPAKIFMGDVGSAVLGLLLALLALLLAR
                     DGVLPLIASLILLAGFWFDATYTLCVRMATGQAFTQAHRSHLYQKLAAFKGHLWTTGV
                     FIAFGLFWLLPLAWLTLRVADLAWVWLVAAVVPIGVASMRFGAGLPAQDEG"
     gene            3115..4995
                     /locus_tag="AC16PP_00390"
     CDS             3115..4995
                     /locus_tag="AC16PP_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012271055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR6480107.1"
                     /translation="MVTDFAVLPIALWTAIALRLGQVNPEVAQFWPAFVVSSLLCIPV
                     FGALGLYRHVVRHTGSEAIWAIVKGATIIALVVAAVAYMIPLPGFPRSVPIIFWLLTL
                     LYVSGTRFSMRAYFQWLQNRVRLRSPAVIYGAGSKGVELARVLSQQGDYLPIAFIDDD
                     RALHKRVIDGLNVYPPKSLGRVLRDSGARDVMVAIGSAEPDERRRIIEFLEPFPARVR
                     LIPDLVELESRRQTIANIRDVEIEDLIGRNTVSPLPHLLRGSVEGRSVLVTGAGGSIG
                     SELCRQIVRQGPQRMVLLDQSEFGLFEIQRELTQLIVSEGLPVDVVAVLGSVTNGTLM
                     RRNMESYAVETVYHAAAYKHVDLVEHNIIQGLRNNTFGTLYTAQAAMDAGVREFILIS
                     TDKAVRTTSVMGASKRLAEMVLQALQEISDSTRFSMVRFGNVLGSSGSVVPLFAEQIA
                     QGGPVTVTHPEVTRYFMTIPEAAELVLQAASMAEGGDVFLLDMGEPVKIRELAERMIR
                     LKGLTVRTSSQSGGDVEIRITGLKPGEKLHEELLVGDEVIGTEHAKIMRAEETFLPWP
                     ALRGALNTLEQACDAYDYDAIKQFIEGLVEGASLESQLGHLNAGPRVLQLDPNRESPS
                     QP"
     gene            complement(5017..>6403)
                     /gene="tcuA"
                     /locus_tag="AC16PP_00395"
     CDS             complement(5017..>6403)
                     /gene="tcuA"
                     /locus_tag="AC16PP_00395"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HIR6480108.1"
                     /translation="ERAPETLAGGNSRFTAGAMRVTYDGVEDLKRLMPELTDAEIART
                     EFGSYPRDAFLDDMGRITHYRTDPELAETLIDSSFSTLVWMRDQGVRFLPLYGRQSFE
                     VDGKARFWGGLTVEAWGGGPGLVEKLTEVARGRGVDIAYSARAVALDHDGQRVVGVRA
                     QTPAGLETIAGDAVILASGGFESNAAWRARHLGPGWDLARVRGTRFNTGDGIRMALEV
                     GAAARGHWSGCHAVAWDLNAPEYGDLSVGDGFQKHSYPLGILVNNAGRRFVDEGADFR
                     NYTYARYGAEILAQPQQTAWQVFDAKVAGLLRDEYRIRAVTRVVADSLQSLAEKMEGM
                     NVSGFRSTVEAFNRAVRTDVPFNPSVKDGRGTVGLAIPKSNWANQLDTAPFEAYGVTC
                     GITFTFGGLHTDSRARVIDQGGEPIEGLFAAGELVGGLFYFNYPGGTGLTSGSVFGRI
                     AGREAAGGRSA"
BASE COUNT          966 a         2059 c         2160 g         1218 t
ORIGIN      
        1 ccacctctgc cggcgcgggt accggcgccg acctgctcat atggatgatc gggtgatgaa
       61 gatcatctac gtggtcaacg aaggacggtt ctttctctct caccgcctgg ctttggccgt
      121 ggaagcaaaa cgtcacgagg tggaggtggt ggtggtttgc ggcgccaata ccggggagca
      181 gggactggcg gacgcggggc tgcgctacgt tacggtgccc atgtcgcgaa gcggtttcaa
      241 tccgttcaag gaactgcgca ccgccgccgc gctgctgcga atctaccgtg ccgagaagcc
      301 cgacctggtt catcacgtga ccatcaagcc ggttctttac ggcacttcca tggcgcgctg
      361 gagcggcgtg cccgccgtgg tgaacgccgt gccggggatg gggttcgtgt ttacccggcg
      421 cgggctgctt gcccgcatcc gacgaagcgt cgtgcagctg ctttaccgtt tgtgcatgtt
      481 ccatcccaac atgcgcgtga tattccagaa caacgaggac atgcaggcgt tcacccagcg
      541 gggcatcgta gaccggcaac acgttgcgct gattcgcgga tcgggcgtcg atctggacgt
      601 gttccggtgc gctcccgagc cgcccggccc cgtgacgttc gtgctcgttg gacggatgct
      661 ccggcacaaa ggggtttacg agtacgtcga ggccgcccgc cgggtcaggc aacggcgccc
      721 cgactggcga ttccagctcg tcggcgacgt ggatcctgga aatcctgcat cgctgcggcc
      781 cgatgagctg atcgcctggc agcaggccgg cgagattgag tggctcggac aacggcgcga
      841 tgttcccgag gtcctggctg cggctcacgt ggtctgcctg ccgtcctatc gggaggggct
      901 gcccaagacg cttctcgaag cggccgcctg cgggcgggcc atgatcgcct cggacatcgc
      961 cggatgccgg gaggtggtcc gcccgaacgt gacgggactg ctggtcccgc cccgggtcag
     1021 cgagccgctt gccgacgcca tgctgcgcct gggcgaggac gatgcgcttc gtcatcgctt
     1081 tgcccgcgcg gcgcgcagcg cggcggagga ggtcttctcc ctcgaggacg tggttcgaca
     1141 tacgttccgg gtctacgacg agttgctgct gccgtgagaa gagccgtcgt caccggcgcc
     1201 ggcggcttca tcggtaccca cctgtgccgc gcgcttcgtc atgagggttg ggaggtggcg
     1261 gtcattaccc gcgctcacgc tctccccgga tgcgagcggc ttcccgtaac gctggcctca
     1321 gccgacgtcg agctgtcgcg ggcgctcggc ggcgcacagg tggtgtttca tctcgccgga
     1381 cttgctcacg gccgggcatc gggcgcggct gaggacgagc tgcatcgggt aaacgtcgag
     1441 tcttccgccc gcctcctcgc cgcgtcggaa gccgccgggg tgccggcgat gatctggctg
     1501 agctcgatca aggtgctcgg agatacgagc gagcggccgc ttcgaccaga ggatgcctat
     1561 cgggctgggg atgcctacgc gcgcagcaaa gtcgaggggg aacgcgcgct tctggaccgg
     1621 gagggcttca cgcgcgcggc catcgtgcgc ccacctctgg tttacgggcc cggcgtggcc
     1681 ggaaacttcg cgagtctcgc gcggtgggtc ggcagcggcg tcccgttgcc gctcggagcg
     1741 gcgtctgcgc cgcgcagcat ggtcggggtc gacaatctgt gcagcctgat gcttcgtctt
     1801 gccgaggaag gctccggcgt gttccacgtt gcggatgctc gcgaccgcag cgtcgcccag
     1861 ctgattcgtg ccatggcggt gctgctgggg gttcccgacc gctcgtggcg ggtttcgccg
     1921 cggctgctgg cggccgtcgc ctcggcattg gggcagcgcg cacgctacac tcggctgttc
     1981 gagcccctgc aggtagatca gagcatgacc cgggagcgcc ttggctggca gccgccccac
     2041 gatcccgatc agcagttgaa ggaaacgatg cgatggtttc gggaacggcg acaggctacc
     2101 tgatcgcggg gttcgcggcc tcgacggtgc tcgtcttcct gatgctccgg gtcggccggg
     2161 cgttgaacct aatggccgtt cccaatgcgc gcagctccca cgtcgtcccc acgccgacgg
     2221 cgggggggtt ggggttcgtg ataccgctcc tggttttttt cgggatgctt taccttgacg
     2281 gcgtcgacgt ggccctcggc ttgctgctca gcctggttct cgtcgcggcg gccagcctct
     2341 gggacgattt tgccgagctt tcggccggtc tgcggtttac ctgtcagacg gctgccgttg
     2401 cgctcatcgt ctggcagctc gatctgccct gggcctggta tctactggga ttgacggggc
     2461 tggcgctgct ctggcacgtc aacctgttca acttcatgga tggcatcgac gggatcgcgg
     2521 cggggcaatg cctgctttac gtggtgggtg tccaggtcct caccctggga gcgcccggct
     2581 ggatgggcga gctttgctgg cttacggccg gcgccatgct gggattcttg gcctacaact
     2641 ggccgccggc aaaaatcttc atgggagatg tcgggagcgc ggtgctgggc ctgctgcttg
     2701 ctctgctcgc gctccttctg gcgcgcgacg gcgtgctgcc gctcatcgcc tctctcatac
     2761 tgcttgctgg gttctggttt gacgcaacgt atacgctgtg tgtcagaatg gcgaccggtc
     2821 aggcgttcac tcaggcgcat cgcagccacc tgtatcagaa gcttgcggca ttcaagggcc
     2881 atctctggac taccggtgtg ttcatcgcct tcggcttgtt ctggttgctg ccccttgcct
     2941 ggctgacgtt gcgcgttgcg gatctggcat gggtctggct tgtggccgct gtggttccca
     3001 tcggcgtggc gtccatgcgt ttcggcgcgg ggctgcccgc ccaggacgag ggctgagaag
     3061 atcatcgaga atttcatcaa tcagttgtcc cggcgcaagc ggcaggctat tgccgtggtc
     3121 acggactttg ccgtgcttcc catcgcgctc tggacagcga tcgccctgcg cctggggcag
     3181 gtcaatccgg aggtggcgca gttctggccg gccttcgtcg tcagcagcct gctgtgcatc
     3241 ccggttttcg gtgctcttgg cttgtatcgc cacgtggtcc gccatacggg cagcgaggcg
     3301 atctgggcca tcgtcaaggg cgcgaccatc attgccctgg ttgtggccgc cgtggcctac
     3361 atgatcccgc tgcccggatt cccccgatcc gttcccatca tcttctggct gctcaccctc
     3421 ctctacgtca gcggcacccg tttctccatg cgcgcttact tccaatggct gcagaatcgg
     3481 gtccgcctgc gcagtcccgc cgtcatctac ggagccgggt cgaagggcgt ggagctggcg
     3541 cgggtgctga gccagcaggg cgactacctc ccgatagcct tcatcgatga cgatcgtgcg
     3601 ctccacaagc gcgtcatcga cgggttgaac gtctacccgc ctaaatcgct cggaagggtg
     3661 ctgcgtgatt ccggagcgcg cgacgtcatg gtggcgatag ggtctgcgga gccggacgag
     3721 cggcgccgca tcatcgagtt tctggagccg tttcccgctc gcgtgcgctt gattcccgat
     3781 ctcgtggagc tggagtcacg ccggcagacc atcgccaaca tccgggacgt ggagatcgag
     3841 gacctgatcg gtcgcaacac ggtatctccg ttgccgcacc ttcttcgcgg ttcggtggaa
     3901 gggcgcagcg ttctcgttac cggagccggc gggtctatcg gctcggagct gtgtcggcag
     3961 atcgttcgtc agggcccaca acgcatggtg ctgctcgatc agtccgagtt cgggctgttc
     4021 gagatccagc gcgagctaac gcagctgatc gtatcggaag ggctgcccgt ggacgtcgtg
     4081 gcggtgctcg gctctgtcac caacggcacg ctcatgcggc gcaacatgga gagctacgcc
     4141 gtagaaaccg tttaccacgc cgcggcttac aagcacgtgg atctcgtcga gcacaacatc
     4201 atccaggggc tcagaaacaa caccttcggc accctttaca cggcgcaggc ggccatggac
     4261 gccggcgtgc gcgagttcat tctcatctcc accgacaaag ccgttcgcac caccagcgtc
     4321 atgggcgcga gcaagcggct ggccgagatg gtccttcagg cgctgcagga aatatccgac
     4381 agtacccgct tctccatggt gcgcttcggc aatgtcctgg gctcgtcggg atccgtggtg
     4441 ccgctgtttg ccgagcagat cgcccagggc gggccggtca ccgtcaccca tcccgaggtc
     4501 acccgctact tcatgaccat tccggaggcg gcggagctcg tcctgcaggc cgccagcatg
     4561 gccgaaggtg gagacgtttt ccttctcgac atgggcgaac ccgtcaagat ccgggagctc
     4621 gcggagcgga tgatcaggct caaagggctg acggtgcgca cttcctctca atcgggtgga
     4681 gacgtggaga tcaggatcac cggactcaag cctggcgaga agctccacga ggagctgctg
     4741 gtgggagacg aggtgatcgg caccgagcac gccaagatca tgcgcgccga ggagacgttc
     4801 ctgccgtggc cggcgctcag gggcgcgctg aacaccctcg aacaggcctg cgatgcctat
     4861 gattacgatg ccatcaagca gttcatcgag ggcctcgtgg aaggggcgag cctggagagc
     4921 cagctcgggc acctgaatgc cggaccgcgc gtcctgcagc tggaccccaa ccgcgagtcg
     4981 ccgtcgcaac cctgaaccgg cgatctggcc acggcctcaa gcagaacgcc cgccggccgc
     5041 ctcgcggccg gcgatcctcc cgaacaccga acctgacgtc agcccggtac cacccgggta
     5101 gttgaaatag aacagcccgc caaccagctc gccggcggca aacagccctt cgatgggctc
     5161 gcccccctga tcgatgaccc gggctcgcga gtcggtatgc aggccgccga aggtgaaggt
     5221 gatgccgcag gtgacgccgt aggcctcgaa gggggcggta tccagctggt ttgcccagtt
     5281 ggacttgggg atggcgagcc cgacggttcc acgaccgtcc tttacgctgg ggttgaaagg
     5341 aacgtcggtt cgaacggccc gattgaaggc ctctaccgtc gaccggaagc cgctcacgtt
     5401 catgccttcc attttttccg caagggattg gaggctgtcg gcgactaccc gggtgaccgc
     5461 gcggatccgg tactcgtcac gaaggagccc ggccaccttg gcatcgaata cctgccaggc
     5521 cgtctgttga ggctgggcga gaatctcggc gccgtatctg gcgtaggtgt agtttcggaa
     5581 gtcggctcct tcgtccacga agcgccgtcc cgcgttgttg acgagaatgc cgagcgggta
     5641 ggaatgtttc tgaaaaccgt cgccgacgct caggtccccg tactcggggg cgttgagatc
     5701 ccaggccacc gcatgacagc ccgaccagtg gccgcgggct gcggccccga cctccaatgc
     5761 catccggatc ccgtcgccgg tgttgaagcg ggtgccgcgc acccgggcca ggtcccagcc
     5821 cgggcccagg tggcgtgcgc gccaggcggc gttggactcg aacccgccgc tggccaggat
     5881 gacggcgtcg ccggcaatcg tctccaggcc ggccggggtc tgcgctcgga cgcccaccac
     5941 gcgctggccg tcatggtcga gggctaccgc gcgggccgag taggcgatgt ccacgccgcg
     6001 cccgcgggcg acctcggtga gcttttcgac cagcccgggt ccgccacccc aggcctcgac
     6061 ggtgagcccg ccccagaagc gcgcctttcc atccacctcg aatgattgcc gtccgtagag
     6121 aggcaggaag cggacgccct ggtcgcgcat ccacaccagc gtggaaaagc tcgagtcgat
     6181 gagcgtctcc gcaagctcgg ggtcggtgcg gtaatgggtg atgcggccca tgtcgtctaa
     6241 gaacgcatcg cgcggatagc tgccgaactc ggtgcgtgcg atttccgcgt cggtgagctc
     6301 cggcatcagc cgtttcaggt cctcgacgcc gtcgtaggtc acccgcatgg cgccggccgt
     6361 gaagcgggag ttgcccccgg cgagagtctc cggggcgcgt tcg
//
LOCUS       DBNHKV010000015         2693 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_710618, whole genome shotgun sequence.
ACCESSION   DBNHKV010000015 DBNHKV010000000
VERSION     DBNHKV010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_710618"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="AC16PP_00400"
     CDS             complement(<1..238)
                     /locus_tag="AC16PP_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HIR6480109.1"
                     /translation="MLNPEQARAAAMELDTAERGRRQIRPTSVTFQGMTVDDAYAVQD
                     AWVKLKIDSGRRIVGRKVGLTSRAMQEAMRIAEPD"
     gene            complement(252..1532)
                     /locus_tag="AC16PP_00405"
     CDS             complement(252..1532)
                     /locus_tag="AC16PP_00405"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6480110.1"
                     /translation="MDSVLTVVGIDPRARVSDNGASRENLAMDHLFDGLKVVDAASFL
                     AGPGAGTVLSDFGADVIKVEPLHGDGYRTLRGNYPIDYNWQLTSRNKRSIALDLDQEE
                     GREVLRTLMADADVLLVNYIGDQLHRFGLAYETVRQHNPGIVYAHLTGYGTEGPDAAK
                     RGFDSTAWWARTGMMDLVRDPGQAPLIGVPGFGDHSSAMSLFGAIMMGLYRRERTGEG
                     SYVSTSLVANGVWANGMQLQGVIAGIDLGAIRQEKGWTNPFTSVYGSSDGRYVLLTVT
                     NQAREWPALCKALGHQEWLEDERFADFRTLFRNRVELIRLISEATETMTLAELTAALD
                     AHGITYGVVSQMAEVVDDPHLRAAGIIVDTASPDESYAATIANPISMAEAPKRSHGRA
                     PEIGEHSREILREHGFTDEQIDELERCRVIGQHR"
     gene            complement(1581..>2693)
                     /locus_tag="AC16PP_00410"
     CDS             complement(1581..>2693)
                     /locus_tag="AC16PP_00410"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR6480111.1"
                     /translation="GVIIVLGSQGLISLEQGIALVFGANIGTCVTAVLASIGKKHRGL
                     QVALVHVTFNVAGVLMWFAFIDELAAFSRWLSPASPGLVGADRLAEEIPRQIANAHTL
                     FNVTNTLIFIWFTGAFAWAVTRLAPGKRSSGAGIFEPRYLDSALVQTPALALDAARRE
                     IVRMGDHLVPMIRRCGTAVLRGSHADLARIRELHGEVDELHVEIVRFLGRVSREEIAP
                     RDSDELHQYLNIATLYESVGDIVETELVRVGAERIRHNVRVSADTENVMSALADKVVW
                     ALETATRAVDLEDASLARDVIAAKESVNRIADEIGSRLMNRLVAEEPHRTSTYRLESQ
                     LIEHYKRVYYLAKRIAKLVASAESASGQDDAQAVIA"
BASE COUNT          483 a          908 c          826 g          476 t
ORIGIN      
        1 aatcgggctc agcgatccgc atcgcctcct gcatggcccg ggacgtgagc ccgactttgc
       61 gcccgacgat gcgtctgccg gaatcgatct tcagcttgac ccacgcgtcc tgcaccgcgt
      121 aggcgtcgtc tacggtcatt ccctgaaacg taactgaagt cggccggatc tgacgccggc
      181 cgcgctcggc ggtgtcaagt tccatggccg ccgcgcgagc ctgctcgggg ttcagcatgg
      241 gcgcggtgtc actacctgtg ctggccgatg acgcggcatc gctccagctc gtcgatctgc
      301 tcgtccgtaa agccgtgctc gcggagaatc tcacgggagt gctcgccgat ctcgggcgcc
      361 cgcccgtgag agcgtttggg ggcctcggcc atgctgatgg ggttcgcgat ggtagcggcg
      421 tagctctcgt cgggagaagc ggtgtcgacg atgatgcccg cggcgcgaag atgagggtcg
      481 tccaccacct cggccatctg gctgaccacg ccgtaggtga tgccatgggc atccagggcc
      541 gccgtgagtt ctgcgagcgt catggtctcc gtggcctcac tgatcagccg gatgagctcg
      601 acccgattcc ggaacagcgt tctgaaatcg gcgaaccgct cgtcttcgag ccactcctgg
      661 tgaccgagcg ctttgcacag cgccggccac tcgcgggcct ggttggtcac ggtcaggagc
      721 acgtaacgac catcgctgga cccgtagacg gaggtgaacg ggttggtcca tcccttctcc
      781 tggcggatgg cgccgagatc gattcccgcg atgacgccct gcagctgcat gccgttagcc
      841 cagaccccgt tcgccaccag cgaggtggag acatagctgc cctcccccgt tcgctcacga
      901 cgatacaaac ccatcatgat ggctccgaac aacgacatgg cgctggagtg gtcgccaaag
      961 ccgggcacgc cgatcagcgg cgcctgcccc ggatccctca ccaggtccat catccccgtg
     1021 cgcgcccacc aagcggtgga atcgaacccg cgcttggcgg catccgggcc ctcggtcccg
     1081 taacccgtaa ggtgggcgta aacgattccg ggattgtgct gcctgaccgt ttcgtacgcc
     1141 aggccgaaac ggtgcagctg atcgccgatg tagttgacga gcagaacgtc cgcatcggcc
     1201 atgagagtgc ggagcacttc ccgtccttct tcctggtcga ggtcgagggc aatgcttcgc
     1261 ttgttgcggg aagtgagctg ccagttgtag tcgatggggt agttgcccct gagggtgcgg
     1321 tagccgtcgc catggagcgg ttcgaccttg atcacgtccg caccgaaatc cgatagcacg
     1381 gtccccgccc ccggcccggc cagaaaagag gcggcatcca cgaccttcag gccgtcaaac
     1441 agatgatcca tagccagatt ctccctgctc gccccattgt cagagacccg cgcccgggga
     1501 tcaatgccga ccaccgtcag aacagaatcc atcgccggat ctcacccgcg caatgaccga
     1561 aacgccgcgg gacgttcggg tcacgcaatc acggcttggg catcgtcctg gccacttgct
     1621 gactcggccg aggcaaccag cttggcaatg cgtttcgcca gatagtacac gcgcttgtaa
     1681 tgctcgatca gctggctttc gagccgatag gtggaggtgc ggtggggctc ctcggccacg
     1741 agtcggttca tcagccggga gcctatctcg tccgcaatcc ggttcacgga ctccttggcg
     1801 gcaatcacgt cacgggccag cgacgcatcc tccagatcga ccgcgcgggt cgccgtctcc
     1861 agcgcccaga cgaccttgtc cgcaagcgcg ctcatcacgt tctccgtgtc cgcgctgacc
     1921 cgcacgttgt gcctgatacg ctcagcgccc acccgcacga gctctgtctc gacgatatcc
     1981 ccgacgcttt cgtagagcgt tgcgatgttc agatactggt gaagctcgtc ggagtctcga
     2041 ggcgcgatct cctccctgga cacgcgcccc aaaaagcgga cgatctccac gtgcagctca
     2101 tcgacctctc catgcagctc ccgaatcctg gccagatccg catgggaccc gcgcagcacc
     2161 gcggtcccgc atcgcctgat catgggcacg agatggtcgc ccatcctgac gatctcccgc
     2221 cgagcggcgt ccagggcaag ggccggcgtc tgaaccaggg ccgagtccag gtaacgcggc
     2281 tcgaagatcc cggcgcccga gctgcgcttc cccggcgcga gccgagttac cgcccacgca
     2341 aatgccccgg taaaccagat gaagatcagc gtattcgtga cgttgaacag cgtatgggcg
     2401 ttggcgatct gcctcgggat ctcctccgcg agacgatccg caccgaccaa ccccggagac
     2461 gcaggagaca gccatcgcga gaaggcggca agctcgtcta tgaaggcgaa ccacatcagc
     2521 accccggcaa cgttgaaggt gacgtggacc agggcgacct ggagcccgcg gtgtttcttt
     2581 ccgatggaag ccagcacggc ggtcacgcag gtgccgatgt tggcgccgaa aaccagagct
     2641 atcccctgct cgagggaaat gagcccctgg ctgcccagca cgatgatcac gcc
//
LOCUS       DBNHKV010000016         9068 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_498187, whole genome shotgun sequence.
ACCESSION   DBNHKV010000016 DBNHKV010000000
VERSION     DBNHKV010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9068
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_498187"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="AC16PP_00415"
     CDS             <1..149
                     /locus_tag="AC16PP_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HIR6480112.1"
                     /translation="LRPKGLIDMLDLKRPIYRNTAAYGHFGRSEPEFTWERTDKAEAL
                     AAAL"
     gene            175..1032
                     /locus_tag="AC16PP_00420"
     CDS             175..1032
                     /locus_tag="AC16PP_00420"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR6480113.1"
                     /translation="MPERGQRPRLSFEFFPPRSEEGFASLARVTAKLDELRPDFYSCT
                     YGAGGSTRDGTKQTVERLVSSGHATAPHLSIGADEEETTFAMLDGYRGMGIDRIVALR
                     GDVPSGIGRSRFRANAQALVGRIRKHSGSHFHIEVAAYPEVHPDARSPGSDLEFFRRK
                     VDAGADSAITQYFYSPHAYYDFVDRCVGAGVTIPIYPGIMPITSYEGLIRFSDNCGAD
                     VPRWLRKHLEAFGDDDDGLRRFGIETVSRLCDDLLRAGAPGLHFYTLNRWGASRAICQ
                     NLGLANRDG"
     gene            1029..1748
                     /locus_tag="AC16PP_00425"
     CDS             1029..1748
                     /locus_tag="AC16PP_00425"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIR6480114.1"
                     /translation="MSRRIYLEASLLPGATVRLDPDHSHYLTRVMRLKAGDALRCFDG
                     SGREHAARLAEPNPRSCSIEIAEELRNAAPATVRLHLAQAWLKGQAMDLVIQKATELG
                     VTDIWPIEALRSNVRIDAGRTAGRLMHWRGIAKSATEQSERLFLPRVHEPRRLEALLT
                     ERPCQRLILLDPGAAALPGALPFDSLVLMVGPEGGWTEHERELAQAAGADRYGLGDLV
                     LRAETAPIAALAAVRQSWGWR"
     gene            complement(1745..2236)
                     /locus_tag="AC16PP_00430"
     CDS             complement(1745..2236)
                     /locus_tag="AC16PP_00430"
                     /inference="COORDINATES: protein motif:HMM:NF013731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR6480115.1"
                     /translation="MEPATAQELIEMSCVMIPLQETNLLLPNICVAEILPWRRVSSIE
                     DAPKWCLGMLEWRGEVIPVVRFERMGDASVEDAEEGRCLIVMNRSRTIEGLPFYALAA
                     EGLPRIVQLTAEDMANVPTNLGVSETMALRVGSEPAIIPNLQYLEVQVANLVRGQETA
                     KQA"
     gene            complement(2247..8162)
                     /locus_tag="AC16PP_00435"
     CDS             complement(2247..8162)
                     /locus_tag="AC16PP_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017902932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR6480116.1"
                     /translation="MATSRENFALDWVKGALQESLDEAIAALDSFAEAGHGEAQMREC
                     MTALHQVHGALVMLELKGTTLLAEHLERLAQHLMIGAVGDSVGAGQCLMQGLLELPGY
                     LDEVQRGLSDAIEPMIPLINEVRGHLDERPLEGAMVTDLGVASEAAIERFDGIDGVEK
                     AGRIRAAYQQVLLCILRGDDRERAVITLRKIAEVLSRVCLGTPHETQWRALGEFAASL
                     ATDTGPIDPDAAKLLRRIDSEIRTLSRDGLGVAGSPVSLDLIKQLLDACRQRGYRSEA
                     ITDISNALAGRGDGGGLAMNGLKALVSASLVVREELALVKDQLDLFARARGRASVDLN
                     SLLAPLKQIRSTLTLLGFASAHEIVTQQLEALEQLASAEAVDPQDLFSVASALVRVDE
                     SMASFTEGDQEVEKITNDAQRTVTVEVRNGLEPVRQAVLEYIAAGWDSEPLAGVPEQI
                     AALCGALEMIPLRRAARILSSCGAYLRRDLLEAGATPGMDAMETLADAITAVDYYLQR
                     TAEDITGGIEDILDVADASLEKLGYAGIVDERPAAQPGAAFQAETAGVEETAEEGFDW
                     EGFVSEAADLREEPASSDAQDQEAPESEPELIDFEPVALEGGSDDEPEAAQAASADAE
                     PEAEPEDASAVEETPVAASAPSPALPPLSPAIAEKTSRLADAFESDEEIVEIFVEEVD
                     EVLETINGCLPRWGEHLSDEDSLTELRRAFHTLKGSGRMVGANIIGELAWSVENMLNR
                     LIDGTFEPTGQFALVVEEATSLMPYLRDAFEAQRAPDLDAMGRIMEKADVLASGGSLE
                     EQAPVAEEEPAVEAAEAGEGFEALGLFVSESEEHLAVLALCDDGEDFVLDEDAYRALH
                     TLAGSAGMAGIEVVSELARPTYEVALAARSEQEVTRASGAVAEFLRAATTALACMIET
                     LRAGSEPEEELQLIADADRLLVEIQETGESKLDELMKLPALAVVMDAEEFLGGWARGV
                     MDLEFAGKLIGALGEIADLARATERSAVADLAAALRATHEELEQETISPESHVALSRA
                     HETLLQQFDAMAAQQTVALATAEIASLTSLREAVTEPSSVEAFGTGAGSSAGSDYAEG
                     AGSRGAEDVAAGDTGTPAEPPAEAGPRVASLPIASLLPEEVDEDIIGIFFEEAEEILE
                     SLDNSIHEWTGEPENRLFLENLLRGLHTLKGGARLSGLSVLGDYTHEFETFLIDFQLS
                     SAPLPEGFFTDLGARYDELEILVSRIRKAIESGERGESFAEAPASATSTPPAEPVAEP
                     ERVAEEDTTGAAAPTDEQPQPAEEAPAQASEVPDLAAAEASAEEAEAEELPAQEIAAS
                     EETDEAEPEPVAEPEPVAEPEPVAAPAPVLRVAPEPSEEAPSAPDPTRADRAKEMVRV
                     GAGLLEELVNLAGESSIVRARIEQGMSDFTGALDEMETTIERVREQLRRLEIETEAQI
                     LFRRDTLEGPGYDEFDPLEMDRYSQLQQLSRGLSESASDMLDLKDTLLFKARESETLL
                     LQQARINTELQEGLMRTRMVPFGRLLPRLRRIVRQVSRELGKDVELHAHNMEGELDRN
                     LLERMVPPLEHMLRNAVDHGIESAEERRRAGKPNTGRIDIRLTREGGDVVIEIADDGA
                     GINVDSVRAKAIERGLMAENSVLGDEEICQFVLAPGFSTAKSVTQISGRGVGMDVVHS
                     EVKQLGGSINIVSRARKGSRFVVRVPFTVSVNRALMVSVGEDLYAIPLNTIEGIVLLS
                     AEELDKVHPPLSKPFEYAGVRYNVRYLGSYLGREYRGNPSQAAVPVVLVRSGDRAVAV
                     HVDAVQGSREIVVKSLGPQFAGVGGVSGATILGDGSVVVILDLLALIRAQKGDGLTVT
                     RRVMPGAAKTRCVMVVDDSVTVRKVTSRLLERQGMDVLVAKDGIEAIAMLQERRPDVM
                     LLDIEMPRMDGFEVARQVRRDERVQDLPIVMISSRTGEKHQEHAAELGVNRFLGKPFQ
                     ENELLATIDSLVN"
     gene            complement(8172..>9068)
                     /locus_tag="AC16PP_00440"
     CDS             complement(8172..>9068)
                     /locus_tag="AC16PP_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR6480117.1"
                     /translation="INFAIDQMRGLVSNINNLSRQVAKAAGDSQETASSLAGAAESQA
                     REIAAASQAINEMAVSIDHVSSNAAESASVAERAVGIANKGSDVVQDTIHGMDVIRGQ
                     IQETSKRIKRLGESSQEIGDIVSLINDIADQTNILSLNAAIQASMAGDAGRGFAVVAD
                     EVQRLAERSSAATKQIAALVKTIQTDTNGAVISMEQTTAEVVNGAKRAQDAGVALEEI
                     ESVSTSLAELIQNISNAARQQAASAGHVSNTMNVIQEITAQTVDSTEQTATSIGNLAA
                     MANEMRESVEGFKLPAQYQSQD"
BASE COUNT         1452 a         3095 c         2856 g         1665 t
ORIGIN      
        1 atttgcgccc gaaaggcctc atcgacatgc tggatctgaa gcgcccgatc tatcggaaca
       61 cggccgcata cggtcatttc ggcagatctg agcccgaatt cacctgggag cgcaccgaca
      121 aggcggaggc actggcggca gcgctgtaac gtcaacccgg agtaacgcgt cagcatgccc
      181 gaacgaggcc agcggccaag gctcagcttc gagttcttcc cgccccgcag cgaggagggt
      241 ttcgcaagcc tcgcccgggt gacggctaaa ctcgacgagt tgcggccaga tttttattcc
      301 tgcacgtacg gcgccggcgg atcgacccgg gacggcacca agcagacggt cgagcggctc
      361 gtgagcagcg ggcacgccac cgcgccgcac ctgtccatcg gggccgacga ggaggagacc
      421 acgttcgcca tgctcgatgg gtatcgcggc atgggcatcg accgtatcgt ggcgttgcgt
      481 ggggatgttc cttcgggcat cggacgctcg cgctttcgcg caaacgcgca agccctggtg
      541 ggccggatcc ggaagcattc cggcagccac ttccatatcg aggtggccgc ctatcccgag
      601 gtccatcccg acgccagatc ccccggctcc gacctggaat tcttccggcg caaagtagac
      661 gccggggccg acagcgcgat cacgcagtac ttctacagcc cgcacgccta ttacgacttt
      721 gtcgaccgct gcgtgggggc gggggtgacc atacccatct acccgggaat catgcccatt
      781 accagctacg agggcctgat tcgtttctcc gacaactgcg gcgcggacgt gccccgctgg
      841 ctgcgcaagc atctggaagc cttcggcgat gacgacgacg gcctgcgccg gttcggcatc
      901 gaaaccgtca gccgcctgtg cgacgacctg ctgcgcgcag gcgctcccgg tctgcatttc
      961 tataccctca accgatgggg cgcgagccgg gcgatctgtc agaacctggg cttggctaac
     1021 cgggacggat gagtcggcgc atctacctgg aggcgtcgct gctgcccggt gcgaccgttc
     1081 gcctcgatcc tgaccattcc cactacctca cgcgggtgat gcgcctgaag gccggggacg
     1141 ctctgcgatg ctttgacggc agcgggcgcg agcatgctgc ccggctggcc gaacccaacc
     1201 cccgctcctg ctcgatcgag atcgccgagg agctgcgcaa cgccgcgccg gcaactgtcc
     1261 ggctgcacct ggcccaggca tggctgaaag gccaggccat ggatctcgtg attcagaaag
     1321 caaccgagct cggggtcacg gacatctggc ccatcgaggc cctgcgcagc aacgtcagaa
     1381 tcgacgcggg gcgcacggct ggccgcctga tgcactggcg gggaatcgcc aagagcgcaa
     1441 cggagcagag cgagcggctg ttcctgccgc gggtgcacga gccccgacgg ctcgaagcgc
     1501 ttttgaccga acgcccctgc cagcggctca tcctgctcga tccaggggca gcggctcttc
     1561 ccggcgcgct gcctttcgac tcgctggtcc tgatggtcgg acccgagggg gggtggaccg
     1621 agcacgaacg ggagctggct caagcggcgg gagccgatcg ctacggcttg ggggatcttg
     1681 tgctccgggc cgaaaccgcg cccatcgccg ccctcgctgc ggtccgacag tcctggggct
     1741 ggcgctaagc ctgcttggcg gtttcctggc ctcgcaccag gttggccacc tgaacctcga
     1801 gatactgcag gttggggatg atggccggct cgctgccaac cctgagggcc atggtctcgg
     1861 acacaccaag attggttggg acgttcgcca tgtcctcggc ggtgagctgc acgatgcgcg
     1921 gcagaccctc cgccgccagg gcgtaaaacg gcagcccctc gatggtgcga gagcggttca
     1981 tcacgatgag gcagcgaccc tcttccgcat cttccaccga cgcatcgccc attcgctcga
     2041 aacgaaccac ggggatgacc tcgccacgcc actccagcat cccgagacac cacttcggcg
     2101 cgtcctcgat gctgctgacc cggcgccagg gcagaatctc cgctacgcag atgttgggta
     2161 gcagcagatt cgtctcctgc agcgggatca tcacgcagga catttcgatg agttcttgtg
     2221 cggtagcagg ttccatgggc ttctctttag ttcaccagcg agtcgatggt ggcgagcagc
     2281 tcgttctcct ggaacggctt gccgaggaat cggttcaccc cgagctcggc ggcatgctcc
     2341 tggtgcttct cgcccgttcg cgagctgatc atgacaatcg gcaggtcctg cacgcgctcg
     2401 tcgcgccgca cctggcgagc cacctcgaag ccgtccatgc gcggcatctc gatatcgagc
     2461 aacataacgt ccgggcggcg ttcctgcagc atggcaatgg cttcgatgcc gtccttggcc
     2521 acgaggacgt ccatgccctg ccgctccagc agccgggagg tcaccttgcg aaccgtgacc
     2581 gagtcgtcca ccaccatgac gcatcgtgtc ttcgccgcgc caggcatgac ccgacgggtg
     2641 acggtcagcc cgtcgccctt ctgcgcgcga atcagcgcaa ggagatcgag gatcacgaca
     2701 acgctgccgt ccccgagaat cgttgcaccg gacacaccgc ccacacccgc gaactgcggg
     2761 cccaggctct tcactacgat ttcccggctg ccctggaccg catccacatg cacggccact
     2821 gcccggtcac cagaccggac gagcaccacc ggcaccgccg cctgagacgg gttgccgcgg
     2881 tattcacggc caagatagct gccgagatat cgcacgttgt agcggacgcc ggcgtactcg
     2941 aagggcttgg aaaggggcgg atgcacctta tccagctcct cggcgctgag caggactatg
     3001 ccttcgatgg tgttgagcgg gatggcgtag agatcctctc ccaccgacac catgagcgcg
     3061 cggtttaccg atacggtgaa gggaacgcga acgacgaagc gggagccctt gcgcgcccgc
     3121 gagacgatgt tgatgctccc gccgagttgc ttgacctcgc tgtgcaccac gtccatgccc
     3181 acgccgcgac cggatatctg cgttaccgac ttggcggtgc tgaatccggg agccagaacg
     3241 aactggcaga tctcttcgtc gcccaggacc gagttttctg ccatcagccc tcgctcgatg
     3301 gccttggcac gaacggaatc gacgttgatg ccggccccgt cgtcggcgat ctcgatcacc
     3361 acgtcgccgc cttcgcgggt cagccggatg tcgatacgcc cggtgttcgg cttgccggcg
     3421 cggcgtcgtt cctcggcgct ctcgatcccg tggtccaccg cgtttcgcaa catgtgctcg
     3481 agcggcggca ccatccgttc caggaggttg cggtccagct cgccttccat gttgtgcgcg
     3541 tgcagctcga cgtccttgcc aagctcacgg gaaacctgtc gcacgatcct tcgcaggcgc
     3601 ggtaacaggc ggccgaacgg caccatgcgc gtgcgcatga gcccttcctg cagttccgta
     3661 ttgatccgcg cctgctggag caggagagtc tcggactcac gggccttgaa cagcagggtg
     3721 tctttcagat cgagcatgtc ggatgcggac tccgacagcc ctcgggagag ctgctgaagc
     3781 tgcgagtatc gatccatctc gagcgggtcg aactcgtcgt aacccgggcc ttccagcgtg
     3841 tcccgacgga acaggatctg cgcttcggtt tcgatctcga ggcgccgcag ctgctcgcgc
     3901 acgcgctcga tggtggtttc catctcgtcc agggcgccgg tgaagtcgct catgccctgc
     3961 tcgatgcggg ccctgacgat gctgctttcg ccggcaaggt tcacgagctc ttcgagcagt
     4021 ccggccccta cgcggaccat ctccttggcc cggtcggccc gtgtgggatc gggcgcgctc
     4081 ggcgcttcct cggacggctc cggggccacc cgcagcacgg gggcaggcgc agcaaccggc
     4141 tccggctctg caaccggctc cggctctgcg accggctcgg gctcggcttc gtcggtctcc
     4201 tcgctggccg cgatttcctg ggccggaagc tcctcggcct ctgcttcctc ggctgatgct
     4261 tcggccgccg ccaggtcggg cacttcgctc gcctgcgctg gcgcctcctc ggcgggctgc
     4321 ggctgctcgt ctgtcggagc ggctgcgccc gtcgtgtctt cttccgccac acgctccggc
     4381 tccgcaacgg gctctgccgg gggcgtcgac gtcgcgctgg ccggcgcctc ggcgaaggat
     4441 tcgccccttt cacccgattc gatggccttg cgaatcctgc tgaccaggat ctcgagctcg
     4501 tcgtaacggg cgcctagatc ggtgaagaag ccctcgggca gcggcgcgct ggataactga
     4561 aagtcgatga ggaaggtctc gaactcgtgg gtgtagtcac cgaggaccga caaccccgaa
     4621 agacgggccc cgcccttgag cgtgtgaagg ccacgcagca ggttctccag gaacagcctg
     4681 ttctcgggct cacccgtcca ttcgtgaata ctgttgtcca gcgattcaag gatctcctcg
     4741 gcttcctcga agaagatacc gatgatgtcc tcgtcgactt cttcaggaag cagcgaagcg
     4801 atgggcaggg aggcgacccg tgggccggcc tccgccggcg gctcggccgg cgtgccggtg
     4861 tcgcccgcgg cgacatcttc tgcgccgcgg gatcctgcac cctcggcgta gtcgctgccg
     4921 gcagaagagc ccgctccggt tccgaacgct tccaccgagg acggttccgt gaccgcctca
     4981 cgcagtgacg tgagcgatgc gatctccgcg gtagccaggg caaccgtctg ctgggcggcc
     5041 atggcgtcga actgttggag cagggtctcg tgggcgcgcg atagagccac atggctctcc
     5101 gggctgatcg tctcctgctc gagctcctcg tgagtggctc gcagcgcggc tgccagatcg
     5161 gcgaccgctg agcgctcagt cgctcgcgcg aggtcagcga tctcgcccag agccccgatc
     5221 agcttaccgg cgaactcgag gtccatgacg ccgcgagccc aaccgcccag aaactcctct
     5281 gcatccatga cgactgccag ggccggcagc ttcatgagct cgtcgagctt gctctctccg
     5341 gtctcctgta tctccaccag cagccgatcg gcatctgcga tgagctgcag ctcttcctcg
     5401 ggctccgagc cggctctcaa cgtctctatc atgcaggcca gcgccgtcgt cgcggcccga
     5461 aggaactcgg caaccgcacc tgaagctcgc gtgacctcct gttcagaccg ggcggcgaga
     5521 gccacctcat aggtaggacg ggccagctcg ctcaccacct caatgcccgc catgcccgca
     5581 ctgccagcca gggtgtgcag cgctctgtag gcatcctcgt cgagaacgaa atcttctccg
     5641 tcgtcgcaga gggcaagcac ggccagatgc tcctcggatt ccgatacgaa caggccaagc
     5701 gcctcgaagc cctcgcctgc ttccgcagct tccacggccg gctcttcctc cgcgaccggc
     5761 gcctgctcct ccagggagcc gcccgacgcc agcacgtcgg ccttctccat gattcggccc
     5821 atagcgtcga gatcgggggc ccgctgggct tcgaacgcat cgcgcagata cggcatcaac
     5881 gacgttgctt cttcaacgac cagcgcaaac tgtccggtgg gctcgaaggt gccgtcaatg
     5941 agccggttga gcatgttctc caccgaccag gccagctcgc cgatgatgtt ggcgccgacc
     6001 atgcggccgc tgcccttgag cgtgtgaaac gcgcgacgca gctcggtaag cgagtcctcg
     6061 tcgctcagat gctcgcccca gcgaggcaga cacccgttga tggtctccag aacttcgtcg
     6121 acttcctcca cgaatatctc gacgatttcc tcgtcggatt cgaacgcgtc ggccagccgg
     6181 ctggtctttt cggcgatggc cggcgacagc ggcggcagcg cgggtgacgg cgcgctggcg
     6241 gccaccggag tctcttccac agcggaggcg tcctccggct ctgcttccgg ctcggcgtcg
     6301 gcggaagcag cctgcgctgc ttccggctcg tcgtccgagc cgccctcgag ggcaacgggc
     6361 tcgaagtcga tgagctccgg ctcggattcg ggcgcttcct gatcctgagc atccgacgag
     6421 gcaggctctt cgcgcaggtc ggcagcctct gagacaaagc cctcccagtc gaagccttcc
     6481 tccgcggtct cttcgacgcc ggcggtctcg gcctgaaacg ctgcgcccgg ctgcgcggcc
     6541 gggcgctcgt cgacgatgcc cgcatacccg agcttctcga ggcttgcgtc cgcgacgtct
     6601 agaatgtcct cgatcccgcc agtaatgtcc tcagccgtgc gctggaggta gtagtcgacg
     6661 gccgtgatgg cgtctgccag ggtttccatg gcatccatgc ccggggtggc acccgcctcc
     6721 agcaggtctc gtcgcaggta tgcgccacag ctgctcagaa tccgggccgc gcgccgcagc
     6781 ggaatcatct ccagagcgcc gcacaaagcg gcaatctgct ccggcacgcc ggcgaggggt
     6841 tccgagtccc aacccgcggc gatgtactcg agcacggcct gtctgaccgg ctcgaggccg
     6901 ttacgcacct ccacggtgac ggtgcgctgg gcgtcgttgg tgatcttttc gacctcctga
     6961 tcgccctcgg tgaagctcgc catgctctcg tctacacgta cgagcgcgct ggcgacgctg
     7021 aagaggtcct gggggtcgac ggcttcggcc gacgccagct gctcgagcgc ttcgagctgc
     7081 tgcgtgacga tctcgtgagc acttgcgaaa ccgagcagcg tcagcgtgct gcggatctgc
     7141 ttgaggggcg ccaagagcga gttcaggtca acgctggcac gaccacgcgc gcgcgcgaac
     7201 aggtccagct ggtctttgac cagcgcgagc tcttcgcgca ccaccaagct tgcgctcacc
     7261 agggctttta agccgttcat ggccagcccg ccgccgtctc cgcggcccgc cagcgcattg
     7321 ctaatgtcgg tgatcgcttc gctgcgatac ccccgctggc gacacgcatc cagaagctgc
     7381 ttgatcaggt ctagagaaac cgggctgccg gcaactccga gaccgtctct ggacagcgtg
     7441 cggatctccg agtcgatccg gcgcagcagc tttgccgcgt ccgggtcgat ggggccggtg
     7501 tccgtggcca ggctcgcggc aaattcgccg agcgcgcgcc actgggtctc atgaggagtg
     7561 cccaggcata ccctggacag cacctcggca atctttcgca gggtgatgac cgcccgttcg
     7621 cgatcgtctc cccgcaggat gcagagcagg acctgctggt aagccgcccg tatccgcccc
     7681 gccttctcta cgccgtcgat cccatcgaag cgctctatgg ccgcttcgct ggcgacgccc
     7741 aggtcggtga ccatcgcgcc ttccaacggc cgctcgtcca gatgaccgcg gacctcgttg
     7801 atcagcggga tcatgggttc gatcgcgtcg gagagcccgc gctgcacctc gtcgagatac
     7861 ccgggcagct cgagaagccc ctgcatgagg cactgaccgg ccccaacgct atcgccgacc
     7921 gcaccgatca tcaggtgctg ggccagccgc tccaggtgct cggccagcag cgtggtgccc
     7981 ttcagctcca gcatcaccag agcgccgtgc acctggtgca gagccgtcat gcattcgcgc
     8041 atctgcgctt cgccgtgacc ggcttcagcg aacgagtcta gcgccgcgat cgcttcgtcc
     8101 agagactcct gcagcgcgcc ctttacccag tcgagggcaa aattttcccg gcttgtcgcc
     8161 ataccgaggt cttaatcctg gctttggtac tgggccggca gcttgaagcc ttcgaccgat
     8221 tcgcgcatct cgttggccat ggcggccagg ttaccgatcg aagtcgccgt ttgctcggtg
     8281 ctgtcaacgg tctgcgcggt aatctcttga atgacgttca ttgtattgga aacgtggccc
     8341 gcagaggcag cctgctggcg tgccgcgttg ctgatgttct ggatcagctc ggccagcgac
     8401 gtggatacgc tttcgatctc ctcgagagcc accccggcgt cctgggcgcg cttggcaccg
     8461 ttgacgacct ctgcggtggt ctgctccatg gagattacgg ccccgttggt gtccgtctga
     8521 atggtcttca ccagagccgc gatctgcttg gttgccgcgc tggatcgttc agccagccgc
     8581 tgcacctcgt cggccacaac ggcaaatcct cgaccggcat caccggccat ggaggcctga
     8641 atggcggcgt tgagggagag aatgttcgtc tggtccgcga tgtcgttgat gagagatacg
     8701 atgtcgccga tctcctggct ggactcgccg aggcgcttga tccgcttcga cgtttcctgg
     8761 atctggcccc gaatcacgtc catgccgtgg atggtgtcct ggaccacgtc cgatcctttg
     8821 ttggcgatgc ccacggcccg ctctgcaacc gatgcagact cggcggcgtt cgaggagacg
     8881 tgatcgatgg agacggccat ctcgttgatc gcctgggacg cggcggcaat ctctcgtgcc
     8941 tggctctcgg ctgcgccagc gaggctggat gccgtctcct ggctgtcgcc cgcggcttta
     9001 gcaacctggc gcgacaggtt gttgatgttg gatacgagcc cgcgcatctg gtcgatggcg
     9061 aagttgat
//
LOCUS       DBNHKV010000017         5506 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_498300, whole genome shotgun sequence.
ACCESSION   DBNHKV010000017 DBNHKV010000000
VERSION     DBNHKV010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_498300"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="AC16PP_00445"
     CDS             complement(<1..640)
                     /locus_tag="AC16PP_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6480118.1"
                     /translation="MTAAREFYVLVGLMVAATVPMIVEIRADHGVGVLQVAAQIWTDA
                     LSAFTGDILNRRPVDRATLTGPVLAGLAFTIVALLPLATLLVLTLVREPPATGQRRVP
                     LREGFRHLLRNGPMKRVLIIALLVHFGESFRNAVSLFFIRDIIGVPTIGAAYFFYFIA
                     GLGAIPFWLWLGRVIGKHRAFMWTLVVVAAVSAANLLLSYGDYLPFFLLFIIK"
     gene            921..1967
                     /locus_tag="AC16PP_00450"
     CDS             921..1967
                     /locus_tag="AC16PP_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6480119.1"
                     /translation="MKVSIGIGGAASGGRRDFEDQVAYVVEAEALGVDTVWTAEAWGQ
                     DAISPLAYLAAKTQRIRLGTGIMQISARTPSMTAMTALTMAAISNDRFILGLGASGPQ
                     VVEGLQGQPFKAPLTRMRETVDIIKLAFAGEKLAYEGKYHRLPLPGGEGKALRLAQPG
                     NDSIPIYLATLGPKSLEYTGEAADGWLGTSFTPEHADAHLGHIRAGAEAAGRSLADVD
                     VQVGGTVAFGDDLAALVAPLKPAMAFTLGAMGSPKTNFYNEAYKRGGWSEAAVEVQQL
                     WVSGRREEAAQRVPDEMIVQANLLGDEAMVRERIRAYRDAGVTTLRVNPMGEGASGRL
                     ETLARVMDMVKALD"
     gene            1998..2327
                     /locus_tag="AC16PP_00455"
     CDS             1998..2327
                     /locus_tag="AC16PP_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 family protein"
                     /protein_id="HIR6480120.1"
                     /translation="MNGTATQAKVIYVLYLAALLNGVTMLIGVVMAYLARGDAPGWLG
                     THYRFQIRTFWMGLLYSMLATMLTPVLIGFFGYLLIAIWLIVRCVKGLKTADREEPME
                     DVETWLF"
     gene            complement(2335..3120)
                     /locus_tag="AC16PP_00460"
     CDS             complement(2335..3120)
                     /locus_tag="AC16PP_00460"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR6480121.1"
                     /translation="MTDPSDLVLLERSGAVATVTLNRPEALNALSAALRDRIAEVFTE
                     IASTPDVEVVILTGAGRAFTVGLDLKELGGEVDPTATTSSRDLSAAIGSLHQPIIGAI
                     NGFAITGGFELALMCDFLIASPQAKFADTHARVGVVPGWGLSQRLPRLIGINRAKELS
                     LTGNYLDAQTACAWGLVNRVVPADELLSTCHRLAEDIVSTDRATRQEIRRIMDAGWGA
                     TLGEGLEIERDANAAHGRSEVRAEKVAARRADIQSRGREQSRD"
     gene            3270..4184
                     /locus_tag="AC16PP_00465"
     CDS             3270..4184
                     /locus_tag="AC16PP_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480122.1"
                     /translation="MQFSDYVTSRALHITSDCLRLLSGSSLEDGVLTAGAASNLLRSV
                     GRLESLWQLEQWCVGKAEYRRALARLRGAEQVLHPRAVWFDGVASQLLARTDDERARR
                     AVERARRAFAQTATGPEAVDPALLNRVFQEESAVWRAQASYSGTRDADLVNYGIGRAF
                     NESRRAVARLEEKPASRKRLRRAHRWVSHIAHHLELLEPALSEANEARAWFVGRLDAN
                     LTRRMDLARFAAVAETLPLKRRQRKRIRQLSLRLDQRLIEQSGKLVTGAYRGGRKKTL
                     RALARDVERLSLGEIALLPLAGRAGASN"
     gene            4209..4472
                     /locus_tag="AC16PP_00470"
     CDS             4209..4472
                     /locus_tag="AC16PP_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480123.1"
                     /translation="MNMTGGDSPHGRRGKEFELRLGAVLMGLLLWSSAASGSAEYLDQ
                     WGPALGIQLPTLQVPDEMGAVRTLDDLTGDEGLLLVLVRSADW"
     gene            4485..4844
                     /locus_tag="AC16PP_00475"
                     /pseudo
     CDS             4485..4844
                     /locus_tag="AC16PP_00475"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            4951..>5506
                     /locus_tag="AC16PP_00480"
     CDS             4951..>5506
                     /locus_tag="AC16PP_00480"
                     /inference="COORDINATES: protein motif:HMM:NF028121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HIR6480124.1"
                     /translation="MKQSRKRGAATRLLNTAVRAALAAALLMLGACTMVMTSVTGSFA
                     EDLSTAILDNPDVAMVRDGAPAYLILIDGLLAQSPDSVELLGQAAMLNSVYAAAFVSE
                     PERSMALHDKALALAERAVCIGIRDGCELRRRDFSDYEAWVARLRAAQVPLAYRLGTT
                     WAGWIQAHSEDFGAIAELGRVKALM"
BASE COUNT          949 a         1705 c         1925 g          927 t
ORIGIN      
        1 ccttgatgat gaacaagaga aagaagggca ggtagtcgcc gtaggacagc aacaggttgg
       61 cggcgctcac ggccgccacc accacgagcg tccacatgaa cgcgcggtgc ttgccgatga
      121 ccctgcccag ccacagccag aaaggaatcg cgccgaggcc ggcgatgaaa tagaagaaat
      181 acgcggcacc gatggtgggc acgccgatga tgtcgcgaat gaagaagagt gaaaccgcgt
      241 tgcggaacga ttcgccgaag tgaacgagca gcgcgatgat gagcacgcgt ttcatgggac
      301 cgttgcggag caggtggcgg aaaccctccc gcagggggac ccgccgctgg ccggtcgccg
      361 gcggttctct caccagggtg aggaccagca gggtggccaa cggcagcagc gcgacgatcg
      421 tgaacgccaa cccggccagc accgggccgg tcaacgtggc gcggtccacg ggtcgcctgt
      481 tgagaatgtc tcccgtgaag gcgctgagcg cgtctgtcca gatctgcgcg gccacctgga
      541 gcacgcccac gccgtggtcg gcccggatct ccacgatcat cggcacggtg gcggccacca
      601 tcagccccac cagcacgtag aactcccggg cagcggtcac ccggctgcgc tgatggcagg
      661 cgggcgacca tcaggatggt gcccaccagc gccagagaga taccgagccc tcccgagtaa
      721 tgggcgggga tccagatcgc cagcgggtat ccgatcacgg caagcggcag gccgaggcag
      781 ccgtgaatcc agatggtggg tctgggcagg ggaagaactg gagacgacgc gtcgtctgcc
      841 gcagcgctca tggctttctc gctcgtcgct ccgtgagcag ggtattatcc acgactttcg
      901 cgcagtggat ggaagccgga atgaaggtca gcatcggcat cggcggggcc gcctctgggg
      961 gccgacgtga tttcgaagat caggtcgcct acgtcgtcga ggcggaagcc ctcggcgtgg
     1021 ataccgtctg gacggcggaa gcatggggcc aggacgccat cagccctctt gcctacctgg
     1081 cggcgaaaac gcaacggata cggctcggga cggggatcat gcagatcagt gcccgcacgc
     1141 cttccatgac cgccatgacg gcgctcacca tggccgccat ctccaacgac cgtttcatcc
     1201 tgggcctggg tgccagcggt ccccaggtgg tcgaagggct gcagggacaa ccgttcaaag
     1261 ccccgttgac gcgcatgcgc gagacggtgg acatcatcaa gctggctttt gccggggaga
     1321 agctggctta cgaaggcaag tatcaccgac tccccctgcc cggcggcgag ggcaaggctc
     1381 tgcgcctggc ccagccgggc aacgacagca ttcccatcta tctggcgacc ctcggaccga
     1441 agtccctgga atacaccgga gaagccgccg acggctggct cggcacgtcg ttcacccccg
     1501 agcatgcgga cgcccatctt ggccacatcc gggccggcgc cgaagcggca ggacgcagcc
     1561 tggccgacgt ggacgtccag gtgggcggca ccgtggcgtt cggcgatgac ctggccgcgc
     1621 tggtggcgcc gttgaagccg gcaatggcat ttacgctcgg ggccatggga tcgcccaaga
     1681 ccaacttcta caacgaggca tacaagcgag ggggctggtc ggaagccgcg gtggaggttc
     1741 agcagctgtg ggtcagcggc aggcgcgaag aggccgcgca gcgggtgccc gacgagatga
     1801 tcgtccaggc caaccttctc ggtgacgagg ccatggtccg cgagcgcatt cgcgcctaca
     1861 gggacgcggg cgtcaccacg ctgcgggtca acccgatggg cgaaggcgca tccggacggc
     1921 tggagaccct cgcacgggtg atggacatgg tcaaagccct cgattgaaac catgaagata
     1981 ttccgggagg gacggtcatg aacggaacgg caacccaggc caaggtcatc tacgtgctgt
     2041 atctggctgc tttgctcaac ggggtcacca tgctgatcgg ggtggtgatg gcctacctcg
     2101 cccggggcga tgcgccgggc tggctgggca cgcactaccg tttccagatc cgcacgttct
     2161 ggatggggct gctctactcg atgctcgcca ccatgctgac gccggtgctg atcgggttct
     2221 tcggctatct gctgatcgcg atctggctca tcgttcgctg cgtgaagggg ctgaagaccg
     2281 cggatcgtga ggagcccatg gaagacgtgg agacctggct cttctgaagc gggttcagtc
     2341 ccgactctgc tcgcggccgc gtgactgtat gtcggctctg cgggcggcga ccttttcggc
     2401 ccgcacctcc gagcgtccat gggccgcgtt ggcgtcccgc tcgatctcga gcccttcgcc
     2461 aagggtcgct ccccatcctg cgtccatgat gcgcctgatt tcctggcggg ttgcccggtc
     2521 ggtggagacg atgtcctcgg ccagccgatg gcacgtcgac agaagctcgt ctgccgggac
     2581 cactcgattg accaggcccc aggcgcaagc cgtctgcgca tccaggtagt tgcccgtcag
     2641 cgaaagctcc ttggcccggt tgatgccaat cagccgcggc aagcgctggg acaggcccca
     2701 ccccgggacc acgcctaccc tggcgtgggt gtccgcgaac ttcgcctgag gagaggcgat
     2761 gaggaagtcg cacatcaggg cgagctcgaa gcccccggta atggcgaaac cattgatcgc
     2821 accgatgatg ggctggtgca gcgacccaat ggccgcgctg aggtctcggg aagacgtggt
     2881 ggccgtcggg tccacctcgc cgccaagctc tttgaggtcc agaccaacgg taaacgcgcg
     2941 tcccgcgccg gtgagaatca cgacctcgac gtctggcgta gaggcgatct cggtgaagac
     3001 ctcggctatt cggtcgcgca gagcggccga cagggcgttc agcgcttcgg gccggttcag
     3061 cgtgaccgtg gcaactgccc cggatcgttc gagaagaacg agatctgatg ggtccgtcat
     3121 gtcgctctcg tcagacaggg tggcgccatt ctagcagagc cgggtggact cccccgcgcg
     3181 gtcgtttgta cgatggggca gacggcgggc cgggcgtcgt ccgtaaggtc gtcaggggac
     3241 gacgtgtggc gccgcggaga agatgagcca tgcaattttc cgactacgtc accagccgcg
     3301 cgctccacat cacgagcgac tgcctgcggc tgctttcggg atcctcgtta gaggatgggg
     3361 tcctgaccgc cggtgcggct tccaatctcc tgcgcagcgt cggtcgcttg gagtccctgt
     3421 ggcagctcga gcagtggtgc gtaggcaagg cggagtatcg acgtgccttg gcgcggctga
     3481 ggggcgcaga gcaggttctg catccgcggg cagtctggtt cgatggggtc gcgtctcagc
     3541 tgcttgccag gacggacgac gagagggccc ggcgagcggt ggagcgtgcc cggagggcgt
     3601 ttgcgcagac ggccaccggg ccagaagcgg tcgacccggc gctgctcaat cgcgtgtttc
     3661 aggaagaatc ggcggtatgg cgggcacagg cttcttactc tgggacgcgc gatgccgatc
     3721 tggtcaatta cgggatcggc cgggctttca acgagtcccg ccgagcggtt gcgcggttgg
     3781 aggaaaagcc ggcgagcaga aaacgcctgc ggcgcgcgca tcgctgggtg agccacatcg
     3841 cccaccacct ggagctgctg gagcctgctc tgtccgaggc caacgaggcc agagcctggt
     3901 tcgtcggccg gctcgatgcg aacctgacca ggcgcatgga cctggcgcgc ttcgcggcgg
     3961 tggctgaaac gctgccgctc aaacggcggc agcgcaaacg catccggcag ctcagcctga
     4021 ggctcgacca gcgcctgata gagcagagcg gcaagctggt caccggcgcg taccggggtg
     4081 gccgcaaaaa aacgcttcgg gcgctcgcgc gcgacgtcga gcgcctgagc ctgggcgaga
     4141 ttgcgctgct gccgttggcg ggccgtgcgg gagcgagtaa ctgaacggat cgagcagggg
     4201 gaaggttgat gaacatgact ggcggcgata gcccgcacgg taggcgcggt aaagagtttg
     4261 agctccggct gggcgccgtt ctgatgggcc tgttgctgtg gtcgtctgcc gcgtccgggt
     4321 cggcggaata cttggatcag tggggtccgg cgctcggtat ccaactgccc acgctgcagg
     4381 ttcctgacga gatgggggcc gttcgaacgc ttgacgacct cacgggtgat gaggggctgc
     4441 ttctggttct cgtccgctcg gccgattggt gaccctattg tcagcgccag ctggtcgagc
     4501 tggccgagtg gaaggatcgt ttcgccgcga tgggcgtcaa cgtcgtgggc atgacctatg
     4561 atcatcccct gctgcttgcc ttctttcacg aagagcacga actcgcctac ccactgctgc
     4621 acgacgagga cggcgcctac ttcgaggccc tggggatccg caatcctgac tacgagccgg
     4681 gagaccgggc gtttggcgtg cctttgcccg gaattatctt cgtccggccg gacggcgtgc
     4741 tggcgctgaa attcgcgcgc ccggggtacc gggaccggcc ggcgttcgaa gaagtctacg
     4801 aggccgttcg cgccgcgctg gctgcgaatg aacctgggcg ttgattttgc tcggtcggct
     4861 tggctttaat gcgcgtgcac gttcccgcac cttagaccag ggccgatgct tttcggcagg
     4921 cggcgacgtt tggagcgtat ttcggcagtc atgaagcaat cgaggaaacg cggggccgcg
     4981 acccgcctct tgaacacggc ggtgcgggcg gcgctcgcag cggccctgct catgctcggc
     5041 gcgtgcacca tggtcatgac ctcggtgacg ggcagtttcg ccgaagacct ttccacggcc
     5101 attctggaca acccggacgt ggcgatggtt cgtgacgggg cgccggccta cctgatcctc
     5161 atcgacggcc tgctggccca gtcgccggac agcgtcgagc tgctgggcca ggcggcgatg
     5221 ttgaactctg tctacgccgc ggcgttcgtc agcgagcccg agcgcagcat ggcgctgcac
     5281 gataaggccc ttgccctggc cgagcgcgcc gtatgcatcg gcatacggga cggctgcgaa
     5341 ctgcggaggc gggacttctc ggactacgag gcttgggtcg cgcggttgcg cgcagctcag
     5401 gtgcccctgg cataccggct gggtacgacc tgggcgggat ggattcaggc ccactccgag
     5461 gacttcggcg ccatcgccga gctcggtcgg gtcaaggcgc tcatgg
//
LOCUS       DBNHKV010000018         5371 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1631843, whole genome shotgun sequence.
ACCESSION   DBNHKV010000018 DBNHKV010000000
VERSION     DBNHKV010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1631843"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="AC16PP_00485"
     CDS             <1..1416
                     /locus_tag="AC16PP_00485"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016837 - carbon-oxygen lyase activity,
                     acting on polysaccharides [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltF family protein"
                     /protein_id="HIR6480125.1"
                     /translation="PEDRPVVQADLESVEVDAQPLLPSVLGAELSEARYDLEGLKQRG
                     LLRVLVGYSHTHYFVDGLTIRGISAENLRQFEPFLQKRLGSPRPRIHVLPIPVARDEM
                     VAFLDQGFADMAVGNITITEERAELVDFSRPLQSDVSELVVHHKDVLNVSSLDDLAGQ
                     AVHVRLSSSFVQSVEALNEDLGRKGLEAIRIVAMDEHLQTEDILELVNAGVIPLTVAD
                     SHMAKFWAGVHDDIVVRDDLAVRSGQKIAWAIRKDLTGLKPIVDDFIRRHREGTLVGN
                     VLLQRYLDRNRYVQNAAASADRKRFEEVAELFQRYSKDYDFDWLLLAAQAYQESRLIQ
                     SLKSPAGAVGVMQLLPATALAPPVQISNIEDLEANIHAGHKYLRHIADHYFDDPEIDE
                     LNLHLLSFAAYNAGPTRISRLRRETARRNLDPNVWFGNVERVVSEKVGREPVTYVRNI
                     FKYYVTYKLMRDQGRSLGEGT"
     gene            1850..2098
                     /locus_tag="AC16PP_00490"
     CDS             1850..2098
                     /locus_tag="AC16PP_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480126.1"
                     /translation="MNEHEERLMFQYGITAETKAVFRYDGYRYERLADAVNYARTQQA
                     PSQMDDTPHHTNDPHGHDSESVTAPDARPSTEFDLGSS"
     gene            2058..2510
                     /locus_tag="AC16PP_00495"
     CDS             2058..2510
                     /locus_tag="AC16PP_00495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HIR6480127.1"
                     /translation="MHAHPPSSTWDLPEILNLKEGFTLSRLGSFRAAGLLLTAATFGS
                     GAAAETPTPGLLVPLHVIEPAQWVVHTSGFERGGEQSFRVSYSDLDLSKAIGVSELYE
                     RLQHASEKVCRLKIRSRSNSEKYLCIRGALAKAVAEVNLLSLNELHSG"
     gene            complement(2606..3136)
                     /locus_tag="AC16PP_00500"
     CDS             complement(2606..3136)
                     /locus_tag="AC16PP_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480128.1"
                     /translation="MDIQGIAQLLGNFGEFFGALAVFATLLYLATQVREAGRSAKLAA
                     VQANRAERIAWFRALRDSPHMIPILQKAAANETLSSEEQSRLLNHVSASWALQYSEWV
                     QREIGSAGEFATSDEHMRWIVADRFHMAWWNQFAESVYPAGFVEYVEALEPAETLDAL
                     WGDAYRQAPQHASEQN"
     gene            complement(3304..3663)
                     /locus_tag="AC16PP_00505"
     CDS             complement(3304..3663)
                     /locus_tag="AC16PP_00505"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR6480129.1"
                     /translation="MGELRRLEELRAEFDRRGVQVITVSTDLPEVIAEERHLHGIQAR
                     MLSDRRLLVTDAFGLRNKAFHSAPPGDGAEALPVPATLLIDTQGKVLWIDISEDYQRR
                     SDPSVVLEAMERYFDSL"
     gene            complement(3673..4065)
                     /locus_tag="AC16PP_00510"
     CDS             complement(3673..4065)
                     /locus_tag="AC16PP_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480130.1"
                     /translation="MKTGTKLGMAALALAFGTVVLWFYLARQVDLPDDRTLFVVVFLC
                     AAALGVSAYVKGTSVVGGIPPAIAIVIGLFLPFTIAVSPQHVEAGKVIQVGDTIPHFS
                     ALDDQGAKFNSTSLNGHLVLMKFFRGHW"
     gene            complement(4519..5061)
                     /locus_tag="AC16PP_00515"
     CDS             complement(4519..5061)
                     /locus_tag="AC16PP_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480131.1"
                     /translation="MKTITDEPHGGDTPKFSVDKYTFNVGEATIDLFDQACRHMFADV
                     VEVREFPSLAETCRLDAILVPDMESLDVTYIGGFDQIHRVTVRYRISVHDPGGATIDE
                     WVLIASAVQRHGGTFTPQREAAIAARLAMVDAIDQFMNGFRERQRIKAWLSQTGKSTN
                     IEDEPTRIFNFPSKIRMPDQ"
BASE COUNT         1168 a         1528 c         1540 g         1135 t
ORIGIN      
        1 cctgaggatc gtccggtcgt tcaggccgac ctggagtccg ttgaggtcga cgcgcaaccg
       61 ctgttgccca gcgtgctggg cgcggagttg agcgaggccc gctacgatct ggaaggcctg
      121 aaacagcgcg gtcttctccg cgtgctggtg ggttactcgc atacccacta tttcgtggat
      181 ggcctgacga tccgcggtat cagtgccgag aacctcaggc aattcgagcc gtttctgcag
      241 aaacggctcg gatcaccaag acccaggatc cacgtgctgc cgatacccgt cgctcgagat
      301 gaaatggtcg ccttcctcga ccagggattc gccgacatgg cggtgggcaa catcacgatc
      361 accgaagaac gggctgagct cgtggacttc tcgcgacctc tgcagagtga cgtctcggag
      421 ctggtcgttc accacaagga tgtcctgaac gtctcctcgc tcgacgacct ggccgggcaa
      481 gcggtacatg tgcgtctttc gagcagcttc gtgcagagcg tcgaagcatt gaacgaagac
      541 ctcggaagga aaggcctcga agcaattcgc atcgtggcga tggatgagca tctgcagacg
      601 gaggacattc tcgagctggt caatgcggga gtcataccgc tgaccgtggc agacagccac
      661 atggctaagt tctgggccgg cgtccacgac gatatcgtag ttcgagacga tctggcggtg
      721 cggagtggtc agaagatcgc gtgggccatc cgcaaggacc tgaccggatt aaaacccatc
      781 gtggacgact tcattcgcag gcatcgggaa ggcacgctgg tcggcaacgt gttgctccag
      841 cggtatctgg accggaacag gtatgtgcaa aatgccgcgg ccagcgcgga ccgcaaacgc
      901 tttgaggaag tggcagaact ctttcagcgc tactcgaagg actacgactt cgactggctg
      961 ctgcttgcag ctcaggctta tcaggagtcg cggctgattc agagtctcaa gagcccggct
     1021 ggtgcggtcg gagtgatgca actgctcccc gccacggctt tggcgccgcc cgtccagatc
     1081 tccaacatag aagatctcga ggcgaatatt cacgcgggac acaaatacct gcgtcacatc
     1141 gccgatcact acttcgatga tccggagatc gacgagctga atctccacct cctctctttt
     1201 gccgcctata acgccggccc gacgcgaatc agccggttgc ggcgcgagac ggcaaggcgg
     1261 aatctggacc ctaacgtctg gttcggtaac gtcgagcgcg ttgtttcgga aaaggtgggc
     1321 cgcgagccgg ttacctacgt ccgtaacatc tttaaatact acgtgacgta caagctcatg
     1381 agggatcagg gacgttcgct gggtgagggg acgtagatcc ccaagttcag cgcagacgtc
     1441 gatggcggcc tgggctgagt ctgccttcgc ctcagagcct tcatttcgca aagatagttc
     1501 gagctatcct cgtctggtac gcgtatcatc gcctcctgat tcgggaacct atgttccgat
     1561 ctcgttcgag cttcacacct cgcctgttca tcggcaaacg cttcttaatc aaaacgaaac
     1621 gccggcatct gtgccggcgc acgcgcgcgc gtgtctcggc taacgggatc acgcgccaga
     1681 agacgctcgc atgggatctg gagccgagaa ttgaaactgt atgctggatg ccttgcctgg
     1741 agcttcacag agactgaccc gtccaagctc ttcctccgag ttctgaacga ctgacgctta
     1801 tggccgggcc gaattggtgg cccggctctc tcaacaacag gttgcgcaca tgaacgagca
     1861 cgaagaacga ctgatgttcc agtatggcat cacggcagag acgaaggccg tgtttcgcta
     1921 cgacggatac cgctacgaac ggcttgccga tgctgtgaac tacgcaagaa cacagcaagc
     1981 tccgtcacag atggacgaca ccccgcatca tacgaatgac ccccacggtc acgactcgga
     2041 aagcgttacg gcgccggatg cacgcccatc caccgagttc gacctgggat cttcctgaga
     2101 ttcttaattt gaaagagggt tttacattgt cgaggttagg tagtttcaga gccgcaggac
     2161 ttttgctgac tgctgcgacg tttggttcgg gggcggccgc cgaaacgccg accccaggcc
     2221 tgctcgtgcc gcttcacgtc atcgagccgg cgcaatgggt cgttcatacg tctggattcg
     2281 agcgcggggg agaacaatcg tttcgcgtct cgtacagtga tctggatctc tctaaggcca
     2341 ttggcgtatc ggagctctat gaacgactgc agcacgcatc agaaaaggtt tgccggctaa
     2401 agatcagatc gcgaagcaac tcagaaaagt acctctgcat caggggggca ctggccaaag
     2461 cggtcgctga ggtgaacctg ctcagtttga acgagttaca ttcgggttga gtcagcgtgt
     2521 aacgggtctg atcagatcac tgcagcgttt caagatgcct cagcaacgag tgatcggttg
     2581 gatcagagag cgttctgtgg ccagactagt tttgctccga cgcgtgctga ggagcctggc
     2641 ggtaagcgtc gccccagagc gcgtcgagtg tctccgccgg ttcaagggct tcaacgtatt
     2701 cgacaaaccc tgccggatag accgattccg caaattgatt ccaccaggcc atgtggaacc
     2761 tgtcggctac aatccaccgc atgtgctcgt cgctcgtggc gaattccccc gcgctcccaa
     2821 tttcccgctg cacccactcc gagtattgca atgcccagct cgcagatacg tgattgagta
     2881 gtcgactctg ttcctcgctg ctcaatgtct cgttggcggc ggctttctgc aggattggaa
     2941 tcatgtgggg cgagtcgcgc agtgcccgaa accatgcgat acgttcagct cggtttgctt
     3001 gcactgccgc taacttcgcc gatctgccgg cctcccgcac ctgtgtagcg agatacagaa
     3061 gcgtggcaaa caccgcgagc gcgccaaaga actcaccaaa gttgcccagc agctgggcta
     3121 taccctgaat gtccatgatc aacctcctcc tgtggatgga gggatggtag cgcagaagac
     3181 gagtggcgga ttttggtatt gatgaaacga ctgcctgtca taaagcgcac ctgggcgctc
     3241 ccttggtcca tgcgggcctg aagcaaatcc cgggtggatg tttgccagag gaggctgctt
     3301 gcactagagc gaatcgaagt acctctccat ggcctcgagc actacggaag gatcggaacg
     3361 gcgctggtaa tcttccgaga tgtcgatcca caaaaccttg ccctgtgtgt cgatcagcag
     3421 agtggccggt accggcagcg cttcggcgcc gtccccaggc ggggccgagt ggaaggcctt
     3481 gtttcgcagg ccaaaggcgt cggtgacgag caatcgccgg tcggacagca ttcttgcctg
     3541 gatgccgtgc aggtgccgtt cttctgcaat cacctcgggc aggtccgtac tcaccgtgat
     3601 tacctgcacg ccgcgccgat caaactcagc gcgtaactcc tccagtcgac gtaactcgcc
     3661 gatacagtag ggtcaccagt gaccgcggaa aaacttcatc aggacgagat gaccgttgag
     3721 gctggtcgag ttgaacttcg caccctgatc atccaacgcc gagaaatggg gaatcgtgtc
     3781 tcccacctgg atgaccttcc ctgcctccac gtgctgaggg ctgacagcga tcgtaaacgg
     3841 cagaaacagc ccgatcacta tcgcgatcgc cggaggaatt cccccgacga cactggtgcc
     3901 tttgacgtag gcagagactc ccagcgccgc agcgcagaga aacacaacca cgaatagcgt
     3961 gcggtcatcg ggcaagtcga cttgtcgggc cagataaaac cacagcacca ctgtaccgaa
     4021 tgcgagggct aatgcagcca tgccgagctt ggttcccgtt ttcatcggta ctcttccgga
     4081 tttggattgg tctcgctcat gacacagggc gttggattta ctccgccaat ttcattcgcc
     4141 tgaagagagc gtcaccggtt agcccttttc cgcggacaac catagagata atcaatgtta
     4201 tcaaatcaaa gaggggaaca gagcacctgc tcgatccgaa tggaattccc gcgtgcgccc
     4261 aagcgttacg gtgagttcgt cggtgctgtc ggcgttgtcg tgacgcttgc ctacttggcg
     4321 gtacagattc ctctgaacac tcgttcgaat cgcatgtccg ccgagcttga atgctcaaaa
     4381 ctgctcaccg actgggtggg gcgagtctca gcgagtagag attcgcagcg atgcgccgga
     4441 aagcagacgt tccggagcct gggcgggggt gatcgctttc ggccccaagc gggcagcaag
     4501 gcgttagtgg gtaggggcct attggtcggg catgcgaatt ttgcttggaa agttaaatat
     4561 tcgagtcggc tcgtcctcaa tatttgtact tttgccagtc tgggaaagcc atgccttgat
     4621 cctttgccgt tctcggaacc cgttcatgaa ttgatctatt gcatccacca ttgccaaacg
     4681 tgcagcgata gctgcctctc tctgcggcgt aaatgtgcct ccgtgtcgct ggaccgccga
     4741 cgcgatcaaa acccattcat cgatggtcgc tccgcccgga tcatgaacgg aaatccgata
     4801 ccggacggtc acgcgatgaa tctgatcgaa cccaccaatg taggtcacgt caagagactc
     4861 catgtccggt accagtatcg catctaatct gcacgtttcg gcaagcgacg gaaactctcg
     4921 aacctcaacc acatctgcga acatgtgtcg acaagcttga tcaaacaggt cgatggtggc
     4981 ctcacccaca ttgaaggtgt atttgtccac cgagaacttc ggtgtgtccc ctccgtgtgg
     5041 ttcgtcggtg atcgttttca ctgcaaaccc cgccgggtag tgcagaccga ccttaatagg
     5101 aagtggcgct acctctggaa gatgagcgag agtggtatcg acatggactg catgcgagca
     5161 agcggctagg ttgaagatcc atatccaaaa gaatgaagaa agttgtcgtt ggcgtcgagg
     5221 cgaggaaccg cgatctggcc gcacgctcat ggacgtttcc tttgaagcct tccctcacga
     5281 tctttcccca gcgatcaaaa aaaaccatag gtatctgcgc gtatccgtat aaatcctgat
     5341 tgagaaaacg ctgcgcggag gaaactctgt a
//
LOCUS       DBNHKV010000019         3069 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_640345, whole genome shotgun sequence.
ACCESSION   DBNHKV010000019 DBNHKV010000000
VERSION     DBNHKV010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_640345"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="AC16PP_00520"
     CDS             <1..1070
                     /locus_tag="AC16PP_00520"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6480132.1"
                     /translation="MTQIKGDSETHPMLSPNDEFADYETYEHLIAAEGIEQQTMFTEG
                     FLGTLSDADRAVLESQEKRIVKNGDYARTGLLRGLVLEERGRGNPYKFGMIGSTDSHT
                     AMASAEEDNFHGKMAIDSTPGTKREDIIPGTPGWDMGAAGLAAVWATENTREGIFDAM
                     KRKEVYATTGPRIELRFFGGFDFAAADAQARDLASVGYGKGVPMGGDLTASPAGNAPS
                     FLVSAVKDPVGANLDRVQIVKGWVDAEGMPQEKIFDVALADGRTVGTDGKVAPVGNTV
                     DVSTGHYTNAIGDPQLTVVWRDPEFDPGKRAFYYVRVLQIPTPRHTLYDAIALGISVE
                     ETGHPATIQERAYSSPIWYAP"
     gene            1142..2041
                     /locus_tag="AC16PP_00525"
     CDS             1142..2041
                     /locus_tag="AC16PP_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR6480133.1"
                     /translation="MNKLLKEPLLHFLVLGLALFVLFDVVSDEKETSDSKLIRVDRET
                     LLTFIQFRTRAFEPSIAAQRLDGLSDQELKLLIDDYIREEALYREALALGMDKNDYVI
                     KRRMIQSIEFITRGFVTRAVEVSDDDVKAHYEANRNDYYVEPFVTFTHVFISGENRSE
                     AEVDALAAAELTELNARQIPFSAAPGRGERFPFFVNYVERDPEFVASHFGADMANAIF
                     ALQPDELVWRGPFLSTYGAHLVLLTRKAPGRFPPLGEIEESVRQDAERAALQKANETA
                     IQAIVDTYEVERQPEPRTRAGAG"
     gene            2038..3060
                     /locus_tag="AC16PP_00530"
     CDS             2038..3060
                     /locus_tag="AC16PP_00530"
                     /inference="COORDINATES: protein motif:HMM:NF025167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HIR6480134.1"
                     /translation="MRTLRSAVLGLWACCAALPAVGHDARPNYVQITETAPNSFNVAW
                     KVPASMPGNALPHPLLPDDCKPQQPPRWHTTGAEHAAQQEFICEQGLSGRVVGIEFPV
                     INPSLSTLYRVRLANGEEHLKILKPDETVWMVPDAEDRLSVAKEYTGLGIEHIWIGLD
                     HLLFVACLLFISRTPRRLLITITGFTVAHSITLALSALDLVRIPTPPVEAVIALSVVF
                     MAWEIVRGDDDSLAYRFPVAVSSSFGLLHGFGFAAVLRDIGLPQTELPTALLFFNVGV
                     EIGQILFVMALLIAFVALRPALGRALRAEPGGAVHWSYLTTPASYVIGTLASYWLIER
                     LAGFWG"
BASE COUNT          541 a         1038 c          961 g          529 t
ORIGIN      
        1 agatgaccca gatcaagggc gactccgaga cccatcccat gctctcgccg aatgacgagt
       61 tcgccgacta cgagacctac gagcacctca tcgctgccga aggcatcgag cagcagacca
      121 tgttcaccga gggctttctc ggcacgttga gcgacgcgga ccgagccgtg ctcgaaagcc
      181 aggagaagcg catcgtcaag aatggcgact acgcccgaac ggggctgctc cgcgggctgg
      241 tgctcgaaga gcgcgggcgc ggcaacccct acaagttcgg catgattggc tcgacggact
      301 ctcataccgc catggcatcc gccgaagagg acaacttcca cggcaagatg gccattgact
      361 ccacgccggg aaccaagcgc gaagacatca ttcccggcac gccgggctgg gacatgggcg
      421 cggcgggctt ggcagcggtg tgggccacgg agaacacccg cgaggggatc ttcgacgcca
      481 tgaagcgcaa ggaggtctac gccaccacgg ggccgcgcat cgagctgcgc ttcttcgggg
      541 gtttcgactt cgccgccgct gacgcccagg cccgggatct ggcctcggtg gggtacggga
      601 aaggggtccc catgggcggc gatctgaccg cgtcacccgc gggcaacgcg ccttcgtttc
      661 tggtgagcgc cgtgaaggat ccagtgggcg ccaatctcga tcgggtccag atcgtcaagg
      721 gctgggtgga cgccgagggg atgccccagg agaagatctt cgacgtggcg ctggcagacg
      781 gccgaacggt gggcacagat gggaaagtcg cccctgtggg caatacggtg gacgtctcca
      841 ccggccatta caccaacgcc atcggggatc cccagctcac cgtggtctgg agggacccgg
      901 agttcgaccc cggcaagcgc gccttctact acgtgcgggt gctgcagatt cccactcccc
      961 gacacacgct ctacgacgcc atcgcgctcg ggatatcggt ggaagagacg gggcatccgg
     1021 cgaccattca ggaacgggcg tactcctcgc ccatctggta cgcaccctga cgacgtttgg
     1081 actctgaccc cccgctgccg cccacccggc ggcagcgagc ggcgtcattt tagagagagc
     1141 catgaacaag cttctcaaag aaccccttct gcacttcctc gttctggggc tggccctgtt
     1201 cgtgctgttt gacgtggtct ccgacgagaa ggaaacgtca gacagcaagc tcatcagggt
     1261 ggatcgcgag accctgctga ccttcattca gttccggacc cgcgccttcg agccgagcat
     1321 cgctgcccag cggctcgacg gcctttccga tcaggagctc aagctgctca tcgacgacta
     1381 catccgcgag gaggccctgt accgcgaagc gctcgccctg ggcatggaca agaacgacta
     1441 cgtgatcaag cgccgcatga tccagtccat cgagttcatc accaggggct tcgtcacccg
     1501 ggccgtggag gtgtccgacg acgacgtgaa ggcccattac gaggccaatc ggaacgatta
     1561 ctacgtggaa ccgtttgtca cgttcacgca cgtcttcata tccggtgaga accgcagcga
     1621 agccgaggtc gacgcgctgg ctgccgccga gctcaccgag ctcaatgccc gccagatacc
     1681 gttcagcgcc gcccccgggc gaggggagcg tttccccttt ttcgtcaact acgtggagcg
     1741 cgacccggaa ttcgtcgcca gccacttcgg ggctgacatg gcgaacgcga tctttgctct
     1801 gcagcccgac gagctcgtct ggcgtggacc atttctgtca acctacggcg cccacctggt
     1861 cctgctgacc cgcaaagcgc ccggccgctt tcccccgctc ggtgagatcg aagaaagcgt
     1921 tcggcaggac gccgagcgag ccgcgttgca gaaggcgaac gaaaccgcca ttcaggccat
     1981 cgtggacacc tacgaggtgg agcggcagcc cgagccccgc acgcgcgccg gcgcaggatg
     2041 agaactctcc gctcagccgt cctgggtctg tgggcgtgct gcgctgcgct gcccgccgtc
     2101 ggccacgatg cgcggccgaa ctacgtccag atcacggaga cggcgcccaa tagcttcaac
     2161 gtggcgtgga aggttcctgc cagcatgccg ggcaacgcgc tgccccatcc tctgctgccg
     2221 gacgactgca agccgcagca gcctcccagg tggcacacca ccggggcaga gcacgcggcg
     2281 caacaggaat tcatctgcga gcagggcctc tccggacggg tcgtgggcat cgagtttccc
     2341 gtcatcaacc cttccctctc cacgctgtac cgcgttcggc tggcaaacgg cgaagagcat
     2401 ctgaagatcc tgaagcccga cgagacggtg tggatggttc ccgatgcgga ggaccgtctc
     2461 tcggtcgcga aggagtacac ggggctcggg atcgagcaca tctggatcgg cctcgaccac
     2521 ctcctgttcg tcgcctgcct gctgttcatc tcgcgcacgc cccgacgcct gctcatcacc
     2581 atcacgggct tcaccgtggc gcactccatc accctggcgc tgtcagctct cgacctggtg
     2641 cgcatcccca cgccgccggt ggaagccgtc atcgcgctca gcgtggtgtt catggcctgg
     2701 gagattgtgc gcggcgacga cgactctttg gcctaccgtt ttccggtcgc cgtttccagc
     2761 tccttcggtc tcctccacgg cttcggtttc gccgccgtgc tgcgcgacat tggcctgccc
     2821 cagacggagc tgcccaccgc gctgctgttc ttcaacgtgg gcgtggagat cggccagata
     2881 ctcttcgtga tggcgctgct gatcgccttc gtagcgctgc ggccggccct tgggcgtgcg
     2941 ctgcgcgctg agcccggcgg agccgttcac tggtcgtacc tgaccacccc ggcaagctac
     3001 gtcatcggca ccctggcgag ctactggctg atcgagcgcc ttgctggctt ctgggggtga
     3061 tcgcgcacc
//
LOCUS       DBNHKV010000020         4158 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_994974, whole genome shotgun sequence.
ACCESSION   DBNHKV010000020 DBNHKV010000000
VERSION     DBNHKV010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_994974"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="AC16PP_00535"
     CDS             <1..882
                     /locus_tag="AC16PP_00535"
                     /inference="COORDINATES: protein motif:HMM:NF015644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HIR6480135.1"
                     /translation="FYVFDEEDLIIQVWQRALPGLFKPRSEMPASLVAHVRYPQDFLL
                     AQGLIFTRYHMSDPDVFYNQEDVWVRATEKHYHDITPVEPYYVMWELPGSDEAEFVLI
                     LPFTPKNRQVLIGWVAALSDGENYGRFIAYKFPKERRVLGPQQVETKIDQDSFLSGQL
                     TLWDQQGSEVIRGNVLAIPIDDSLLYVEPIYLRAETAAYPELRLVAVMQGDNLSYAET
                     LDEALLGLFTGRQLVGETPSRSVKGLAIEARMAFESYLEALGDKRFEAAGEALRRLSQ
                     LLERIDAAASVGEGNGP"
     gene            940..1806
                     /locus_tag="AC16PP_00540"
     CDS             940..1806
                     /locus_tag="AC16PP_00540"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF016032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HIR6480136.1"
                     /translation="MPSTCTPRAFGELHNASLDVSIDTGYPPVINSRREAELAFRAAR
                     EVVGDVGLMQLDHPSMGAEDFSYYLERIPGCVLLMQVASSDLGKDLGQSVARPAESCR
                     TLGMRLVGAGTHPFSTRLAAITPGPRYESMERTSGLLGHRQITFSTHVHLSVIDGDEA
                     LWLARQLAPYLPLMLALSASSPFWQGHDTRFAAFRPRILASRFGLDARVAVNENGDVM
                     TVREVARQTLDLIADTAARFGDTDHLSRLARAISAGLPSERQRRRFAATGSLRAVVDA
                     VIDELLRDPVVG"
     gene            complement(1822..2760)
                     /gene="trxB"
                     /locus_tag="AC16PP_00545"
     CDS             complement(1822..2760)
                     /gene="trxB"
                     /locus_tag="AC16PP_00545"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR6480137.1"
                     /translation="MTATHHRLIVLGSGPAGYTATLYAARANLRPALITGVEVGGQLT
                     TTTEVENWPGGHAELQGPELMAQMAEHVERFEARVINDYITTADLEAKPIRLQGDSQY
                     TCDALIIATGASAMYLGLPSEEAFLGRGVSACATCDGFFYRGQAVAVVGGGNTAVEEA
                     LYLSNICSKVYLVHRRDELRAEKILQDRLFARENIEPVWNHTLAEVVGDDSGVTGVRL
                     DGTHGAEPMDLDVTGVFIAIGHRPNTEIFAGQLEMEGGYITTRSGLDGNATATSTPGV
                     FAAGDVADHVYRQAITSAGFGCMAALDAEKYLDESV"
     gene            2888..3286
                     /locus_tag="AC16PP_00550"
     CDS             2888..3286
                     /locus_tag="AC16PP_00550"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6480138.1"
                     /translation="MDIQDRIDRDGGVLERLNISVDLSANAGVRLEMKVAEDMVNAGG
                     YCHGGLLFALADTACAYALAEAGLAPVTLDAGITYLRPATAGERIAAETEVVRAGRRI
                     GHCEVRLTREDGTLLAIYRGTCSNASSDSR"
     gene            3432..4001
                     /locus_tag="AC16PP_00555"
     CDS             3432..4001
                     /locus_tag="AC16PP_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HIR6480139.1"
                     /translation="MQIPLQITWRNIPKSEALEADIVAKAEKLEEFFDHILSCRVVVE
                     KSHGRHRKGNLYRLRLDIHVPDKEIVVTRDPGDDHAHEDMYVSIRDAFDAARRQLQDY
                     VRVRRGHVKPHDQHHVGRVLRRIAAEDYGFLGTADGREIYFHRNSVVNADFDKLEPGT
                     EVMFVEETMNEGVQAKMVSVGKHRFPEGS"
BASE COUNT          711 a         1299 c         1377 g          771 t
ORIGIN      
        1 ttctacgtgt tcgacgaaga agacctcatc attcaggtgt ggcagcgcgc gctgccgggc
       61 ctgttcaagc cccgctccga aatgccggcc agcctggtcg cccacgtgcg ttatccccag
      121 gacttcctgc tcgctcaggg gctcatcttc acccgctacc acatgtccga tcccgacgtc
      181 ttctacaacc aggaagacgt ttgggtgcga gccaccgaga agcactatca cgacatcacg
      241 ccggtggagc cctattacgt gatgtgggag ctgccaggct cggacgaagc ggaattcgtg
      301 ctcatcctgc cgttcacgcc caaaaaccgg caggtgctca taggctgggt tgccgcgcta
      361 tccgatggcg agaactacgg gcgcttcatc gcttacaagt ttcccaagga acggcgggtg
      421 ctcgggcctc agcaggtcga aaccaagatc gatcaggact cgttcctgtc ggggcagctg
      481 acgctctggg accagcaggg ctccgaggtg atacgcggga acgtcctggc aatacccatc
      541 gacgactcgc tcctgtacgt cgagcccatc tatcttcgcg ccgagaccgc cgcgtatcct
      601 gagctgcgac tggttgccgt gatgcaggga gacaatctga gctacgccga gaccctggat
      661 gaggcgctgc tgggcctctt cacgggccgg caactggtcg gggagacgcc gtctcggtcc
      721 gtcaaaggtt tggccatcga ggcgcgaatg gctttcgaaa gctatctcga ggcgctggga
      781 gacaagcgct tcgaagcggc gggcgaggcg ttgcgccggc tcagccagct gctcgagcgg
      841 atcgatgcgg cagcgtcggt tggggaagga aacgggccgt gacgcaatgg gtcgattcgg
      901 cggtgaacgc cggtacgatc acggcccaat cggaccgatt tgccatcgac gtgcacgccc
      961 cgggcgttcg gcgagctgca caacgcgtcc ctggacgtat ccatcgacac gggctacccg
     1021 ccggtgatca acagccgccg ggaggccgag cttgcctttc gggccgctcg cgaagtggtt
     1081 ggcgacgtcg gcctgatgca gctcgatcac ccgagcatgg gtgctgagga tttctcctac
     1141 tacctggaac ggattcccgg ctgcgttctc ctcatgcagg tcgcgtcgag cgatctcggc
     1201 aaggacctcg gccaaagcgt cgcccgacct gccgaaagct gccggacgtt gggaatgcgg
     1261 ctggtcggcg cggggacgca tccgttctcc accaggcttg cggccatcac gccaggcccc
     1321 cgatacgaat ccatggagcg gacgtcgggg ctgctcgggc atcgtcagat caccttttct
     1381 acccacgttc acctgagcgt catcgatggg gacgaggccc tatggctcgc caggcagctg
     1441 gccccttacc tgccgctgat gctggcgctc tcggcgagct cgccattctg gcagggtcac
     1501 gacacccgtt ttgccgcttt ccggccgcgg atcctggcct ctcgtttcgg cctcgacgcc
     1561 cgggtggccg tcaacgagaa cggcgatgtg atgacggtgc gcgaggtcgc acgtcagacg
     1621 ctcgatctga tagccgacac ggctgcgcgg ttcggagaca cggaccacct ttccaggctg
     1681 gcacgagcga tctccgcagg gctcccatcg gaacgccagc ggcgtaggtt tgcggcgacc
     1741 ggctcgttgc gggcggtggt ggatgccgtc atcgacgagc ttctgcggga ccccgtggtg
     1801 ggatgatacc gggcctgccg ctcagaccga ctcgtccaga tatttttcgg cgtccagcgc
     1861 ggccatgcag ccgaacccgg cggaggtaat cgcctggcgg tagacgtgat cggccacgtc
     1921 cccggctgcg aatacccccg gggtgctggt cgcggtggcg ttgccgtcca gaccgctgcg
     1981 ggtggtgatg tagccgccct ccatctcgag ctggccggcg aagatctcgg tgttcggccg
     2041 gtggccgatg gcgatgaaca cgcccgtcac gtccagatcc atcggctcgg caccatgggt
     2101 gccgtccagg cgcaccccgg tgacgccgga atcgtcaccg accacctcgg ccagggtgtg
     2161 gttccatacc ggctcgatgt tctcccgggc aaacaggcgg tcctgaagga tcttctccgc
     2221 gcgcagctcg tcgcggcggt gaacgaggta gaccttgctg cagatgttcg acaggtacag
     2281 cgcctcttcc acggccgtgt tgcctccgcc gaccacggcg acggcctggc cgcgatagaa
     2341 gaaaccgtcg catgttgcgc aggcgctcac gccgcgaccc agaaaggcct cctccgaagg
     2401 cagcccgaga tacatggctg acgcgccggt cgcgatgatc agggcatcgc acgtgtactg
     2461 ggagtctccc tggaggcgaa tgggcttggc ctcgaggtct gcggtggtga tgtagtcgtt
     2521 gatgaccctc gcctcgaagc gttccacgtg ttcagccatc tgggccatca gctcggggcc
     2581 ctggagctca gcgtgcccgc cgggccagtt ttccacctcg gtcgtcgtgg tcagctgccc
     2641 gccgacctcc accccggtaa tcagcgcggg tcgcaggttc gcccgggccg cgtacagcgt
     2701 tgcggtgtat ccggccggcc ctgatccgag cacgatgagt cgatgatgtg tggcggtcac
     2761 ggttgtgtcc tgtggatcag cgagcgggca ttttatacag cgcacgccca tgggtgaacg
     2821 ctcacgcgtt gactccggct cggtattgga ggtttattgg gcctattgat tcgcgggcgg
     2881 ggccagcttg gatattcagg atcgcatcga ccgcgacggc ggtgtcctcg agcggttgaa
     2941 catcagcgtc gatttgtcgg cgaacgccgg cgtcaggctc gagatgaaag tggcggaaga
     3001 catggtcaac gcgggtggat actgtcacgg gggtctgctc ttcgcgctgg cggacaccgc
     3061 gtgcgcctat gccctggccg aggcaggatt ggcgccggtg acactcgatg cgggcataac
     3121 ctaccttcgc ccggcgaccg cgggtgaacg gattgccgcc gaaaccgaag tcgttcgcgc
     3181 ggggcggcgg atcggacatt gcgaggtccg gctcacgcgc gaggacggaa ccctgctggc
     3241 catctaccgg gggacctgct ccaatgcttc ctcggattct cgctaacccc ggcagccggg
     3301 gttcgcgggg cgtgccgggt tcggctgcac gcctaccggc ccccgcgggg aactacgtgg
     3361 aaatccggag cgccaatcgg aacccaaagg cgcatctttg gacgtgtgta gaacgtgatg
     3421 ggagttgcga cttgcagata cccttgcaga ttacctggcg aaacatcccc aaaagcgaag
     3481 ccctggaggc cgacatcgtc gcgaaggcgg agaagctcga agagtttttc gaccacatct
     3541 tgagctgccg tgtggtggtg gagaagtctc atgggcggca ccggaagggt aatctctacc
     3601 gcctgcggct cgacatccac gtcccggaca aagagatcgt ggtgacgcgt gatccggggg
     3661 acgatcacgc ccatgaagac atgtacgtga gcatccgcga tgccttcgac gccgctcggc
     3721 ggcagctcca ggactacgtc cgcgtcaggc gcggacacgt caagcctcac gatcagcatc
     3781 atgttggccg ggttctgcga aggatcgccg cggaggacta cggctttctc ggaacggccg
     3841 acggtcgcga gatctacttt catcgtaact cggtggtcaa tgcggacttc gacaagctcg
     3901 agcccggtac cgaggtgatg ttcgtggagg aaaccatgaa cgaaggcgtt caggcgaaga
     3961 tggtttccgt gggcaagcac cgatttcccg aaggttcctg atctcggtgg cggaatgccg
     4021 gacggctcag ccgccgccat ccccgccggc agagtgccgt cccgccgcgt gatcggcaat
     4081 gggtggggtc gtgaatttct gtctccgccc tgtcgattgg aagcaggtag tgccgattgg
     4141 ttggtgatcg ccaacgcg
//
LOCUS       DBNHKV010000021         9430 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1419999, whole genome shotgun sequence.
ACCESSION   DBNHKV010000021 DBNHKV010000000
VERSION     DBNHKV010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9430
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1419999"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="AC16PP_00560"
     CDS             <1..543
                     /locus_tag="AC16PP_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6480140.1"
                     /translation="AFEADERVVLLIDEIDKADIEFPNDLLQELDRMEFFVYELRETI
                     QAKYRPVVIITSNNEKELPDAFLRRCFFHYINFPDRETMQQIVEVHHPSIKSELVKEA
                     MEIFFDVRKVPGLKKKPSTSELIDWLKLLMADDIPDEILTNRDTSKAIPPLYGALVKN
                     EQDVHLLERLAFMNRRDSRG"
     gene            585..1757
                     /locus_tag="AC16PP_00565"
     CDS             585..1757
                     /locus_tag="AC16PP_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012722828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6480141.1"
                     /translation="MLINFFLTLRKYKVPVSIRELMDLLEALSLRLVYANIDDFYLLS
                     RTCMVKDEKHYDKFDRAFAAYFKGLEDLHGMLESLIPDEWLRREFEKSLTAEELEKIQ
                     SLGGLEKLIEAFQEARAEEERRQAEQARQGEEGEEGENPDRQGQKGPGGLGKGKKKKA
                     KKVWDQRQYKNLDDSVELGTRNIKMALRRLRKFARSGKEEELDIDTTIRSTAHNGGML
                     DIRMRPERKNTVKVLLFLDIGGSMDSHVKVCEELFSATRTEFKHMESFYFHNFIYESV
                     WKDNRRRMNERLSTWEILNKYGQDYKVVFVGDATMAPYEITHAGGSVEHWNEEPGAEW
                     MNRFTEIYDRLVWINPTPQETWEYSSSVGMTQQLVNGQMYPLTIRGLEEGMSFLSK"
     gene            1777..5652
                     /gene="hrpA"
                     /locus_tag="AC16PP_00570"
     CDS             1777..5652
                     /gene="hrpA"
                     /locus_tag="AC16PP_00570"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HIR6480142.1"
                     /translation="MASRRADTSFDPAAVMRRDVHRLKSLRRSNPSAWQALREQSEAR
                     LLARRGQRLEIAYPPELPISQHADEITRLLADHQVIVVAGETGSGKTTQLPKMCLEAG
                     FGYRGMIGHTQPRRLAARAVAARVAEELGARVGEAVGYAVRFSDQVGEHTLVKLVTDG
                     LLLNEIRTDRFLDAYEVIIVDEAHERSLNVDFLLGYLRRLVDRRHDLRVIITSATIDV
                     ASFSEHFGGAPVVEVGGRGFPVETRYADEEALADRSAEELIVDRIRSIEKTPVAGSAR
                     DILVFQSGEREIFDTAHALRQALDERYEILPLYARLSARDQQRIFKPSGYRRVVIATN
                     VAETSLTVPNIGFVIDPGFARVSRYSFRSKLQRLPVEPISQASADQRLGRCGRVAPGV
                     CYRLYTENDYLSRPPYTDPEIKRTNLAQVVLQMRSLGLGKVAGFPFLDPPDPRAVRDA
                     ERLLHELGALEGGGLSKTGRMMARLPVDPRLARMIIAGHEHRSLSEVLVIVSALAAQD
                     PRERPLERQGSADRAHSEFSDPRSDFLAFLNIWQWYLEARQRLSRARLSRELGSRFIS
                     ATRMREWHALHRQLLLACRELALKPNDQPADYSAVHRALLQGSLSLVGLHEEKGSYLG
                     PRNLRFRIFPGSALARRGPKWLMAGEITETRRVYARCVAGVEARWIEEAAGDLVKRRY
                     SEPRWSVRRGEVIALETVTLYGLPLADGRRVSYRKVDPDACRELFITEALVRGGLEGP
                     FEFLLHNRGLIREIVDEESKGRRRDLLIGDAAQADLYAARLPREVCSVSTLGKFLKQA
                     GPEARERLKFSREELLGVASVELPETDYPGALEIRGVAFPLSYRFAPGEPDDGVSITV
                     PVGMLNSLVGEALEWGVPGFFDVLVEQWLRSLPKTKRRLLAPLPDRLGDIVAVLCAAD
                     RFRHGRLLPALGEVLADNFGLKVASTDWDRGRVDPHLLMNIRIVDGSGNLLAQSRDLG
                     ELRSRFAAEIEERMASSAAAELENTGLTAFPEGGLAESVSLKDRSGQTIAYPALLDAG
                     DSVSVKLFAEARERDRANRDGFARLALIAVAQTTRHLKKGLDEQRRLGLLYAALGGAR
                     QLADELLRAAAWRCFFADRPLPRSEADFEQRIRTNRADLADTFSVMLEVFKTVLEKRF
                     EIVTLCETLTSPAFREAVGDVATQLDELVPADVLSRVPPARLADLPRYLDAARYRLEH
                     LQGRVKRDEENTRLVASFRDRLRRLEADPAATDGEVAELRFAVEELRASLFAEPLAAR
                     WAAGGDRSRVSPKRLDRSFEALERALGLI"
     gene            5813..6094
                     /locus_tag="AC16PP_00575"
     CDS             5813..6094
                     /locus_tag="AC16PP_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HvfA family oxazolone/thioamide-modified RiPP
                     metallophore"
                     /protein_id="HIR6480143.1"
                     /translation="MFNRKMKPIAAVIGTAFVASLAATSVANAASPFAANELDRGYLL
                     LADGHAEGKCGEGKCGEDKDAEGACGEDKDAEGKCGEGKCGEGKCGGAA"
     gene            6118..6960
                     /locus_tag="AC16PP_00580"
     CDS             6118..6960
                     /locus_tag="AC16PP_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HvfB family MNIO-type RiPP peptide maturase"
                     /protein_id="HIR6480144.1"
                     /translation="MSSPVHGAGLGLRRGLLAELASMENASVDFMEIAPENWIGVGGR
                     FGRQFRALCERFPMVCHGLSLSIGGPAPLDIDFLKDIRVFLDEHGIDCYTEHLSYCSD
                     DKGHLYDLMPIPFTAEAVRYVAARVRQVQDVLGRRIGIENVSYYVAPGRELTELEFIN
                     AVLAEADCDLLLDVNNIYVNSINFGYDPVDFLNRIDGARSVYVHVAGHYVEAPDLRVD
                     THGSAVAEPVWDLLSAAYNRWGALPTLLERDFNFPPLGELLHEVSRIRSLQAAVRSGH
                     AANG"
     gene            6944..7723
                     /locus_tag="AC16PP_00585"
     CDS             6944..7723
                     /locus_tag="AC16PP_00585"
                     /inference="COORDINATES: protein motif:HMM:NF050145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HvfC family RiPP maturation protein"
                     /protein_id="HIR6480145.1"
                     /translation="MPRTGENLPEFRARQLDFAAHLRNPDLYPCPEGLDARRMRIYVD
                     LVYNNVESFLANGFPVAKEILGDASWHALIRDFLHRHPSESPYFLEIPQEFLAFVSSC
                     DRSEVPDYFLELCHYEWVELALATAEDDPPEAGVDPSGDLVHGRPVVSPLIWPLAYRY
                     PVHRIGPGFRPDAPPESPTHLIVFRRRDDSVGFLEASAATLRLVELLSEGATGASAIE
                     RLCQEMPGLDSQVVHDEGVATMMRLREAGIILGTEIAGASE"
     gene            7716..8450
                     /locus_tag="AC16PP_00590"
     CDS             7716..8450
                     /locus_tag="AC16PP_00590"
                     /inference="COORDINATES: protein motif:HMM:NF016236.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0120010 - intermembrane phospholipid
                     transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HIR6480146.1"
                     /translation="MSDGRARGRARFTTILLCALTPLVTPMSGAAQVNPGEAANRGVY
                     GVNDRADRALLRPVAKGYTKAVPGFLRRAVHNVLLNLGTPLVAVNQLLQGKPNEAFSD
                     SGRFLVNSTVGIAGIFDPATKLGIEEHTEDFGQTLAVWGVPSGPYMVVPFWGPSYLRH
                     AFGMIVEFFASPLRLISPARDRYIAYGVTILDRRARLIGPDEMIAGDDYLFVREAYQQ
                     RREYLILDGAVEEDPFMMDEAWQEEP"
     gene            8590..>9430
                     /locus_tag="AC16PP_00595"
     CDS             8590..>9430
                     /locus_tag="AC16PP_00595"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR6480147.1"
                     /translation="MRTQLDRLGLDPIPAADLKFASEICEHSPPDLVISGDLDWAERL
                     ADMHRRLPVVLAVDRNPERDVLIRALRCGLADVWTLPAEDDFLRGRVDDILRNNEAAA
                     SQAEKRLGQYVATLQRDQRAGRYIQMGMLPPSPMAIDRFRFQHRIVPSLMLSGDFVDY
                     FRLTDRHFVFYIADVSGHGASSAFVTVLLKNFSRRLRREYRPSMLQAPGEILEWFNRE
                     LLEQQIDKHVAMILGLGDLEKNVVSLVNAGHFPPAIHVAGQSHAVYVEQRGKPVGLFE
                     TVSY"
BASE COUNT         1689 a         2875 c         3051 g         1815 t
ORIGIN      
        1 gccttcgagg ccgacgagcg tgtggtgctg ttgatcgacg agatcgacaa ggcggacatc
       61 gagtttccga acgacctgct ccaagagctc gaccggatgg agttcttcgt ctacgagctc
      121 cgcgagacga tccaggcgaa gtatcggcct gtcgtgatca tcacgtcgaa caacgagaag
      181 gagctgccgg acgccttcct gcgccgctgc tttttccact acatcaactt cccggatcgg
      241 gagaccatgc agcagatcgt cgaggtgcat catccgagca tcaagtccga gctggtcaag
      301 gaagcgatgg agatcttctt tgatgtgcgc aaggtgccgg ggctgaagaa aaagccgtcc
      361 acatccgaac tcatcgactg gctgaagctg ctgatggcgg acgacattcc ggacgagatt
      421 ctcaccaatc gggatacgag caaggccata ccgccgcttt acggcgccct ggtgaagaat
      481 gaacaggacg tgcacctgct cgagagactg gcgttcatga atcgtcggga cagccgcggc
      541 tagaagcggc gggtcgatag ccaccgaggg agcacgaccg gacaatgctc atcaacttct
      601 ttctcaccct gcgcaaatac aaggtgccgg tctccatccg cgagttgatg gatctgctgg
      661 aggcgctcag ccttcggctg gtttacgcca acatcgacga tttctatctg ctcagccgca
      721 cctgcatggt caaggacgag aagcactacg acaagttcga ccgggccttt gcagcctact
      781 tcaaagggct ggaggacctg cacgggatgc tggagtccct cataccggac gagtggctgc
      841 gtcgcgaatt cgaaaaatcg ttgacagccg aagagctcga aaagattcaa tccctcggcg
      901 gcctggagaa gctcatcgag gcgtttcagg aagccagggc cgaggaggaa cggcgtcagg
      961 cagagcaggc gcgtcagggc gaggaaggcg aggagggcga gaatcccgac cggcagggcc
     1021 agaagggccc gggcggcctt ggcaaaggca aaaagaagaa agcaaagaaa gtctgggatc
     1081 agcggcagta caagaatctc gacgactcgg tggagctcgg cacccgcaac atcaagatgg
     1141 cgctgcgccg gctgaggaag tttgcccgtt ccgggaagga agaagagctg gatatcgaca
     1201 ccacgatccg gtcgacggcg cacaacggcg gaatgctcga catacgcatg cggcccgagc
     1261 gcaagaatac ggtgaaggtg cttcttttcc tcgacatcgg cggttccatg gactctcatg
     1321 tgaaggtctg tgaggagctt ttctcggcga cccggaccga gttcaagcac atggaaagct
     1381 tctacttcca caacttcatc tacgagtcgg tgtggaaaga caaccgccgg cggatgaacg
     1441 agcgtctgtc gacgtgggag atcctcaaca agtacggcca agactacaaa gtggtcttcg
     1501 tgggagacgc caccatggct ccctacgaga ttacccatgc cggtggtagc gtggagcact
     1561 ggaacgaaga gcccggcgcg gagtggatga accgcttcac cgaaatttac gaccggctcg
     1621 tctggatcaa tcccactcct caagaaacct gggagtattc ctcgtcggtc ggcatgaccc
     1681 agcagcttgt gaacgggcag atgtaccccc tgacgattcg gggcctcgaa gagggaatga
     1741 gcttcctttc caaatagcct ggccgaccat ctcacgatgg cttctcgacg cgccgatacc
     1801 tcgtttgatc ccgctgccgt catgcggcgg gacgtccatc gtctcaaatc actgagaagg
     1861 agtaatcctt ccgcctggca ggcgcttcgt gagcaatccg aggccaggct cctcgcccgg
     1921 cgcggccagc ggctggagat cgcttacccg cccgagcttc ccatcagtca gcatgccgac
     1981 gagatcacga ggctgctggc cgatcatcag gtcatcgtcg tggccgggga gaccgggtcg
     2041 ggaaagacca ctcagctgcc caagatgtgc ctcgaggcgg gcttcggcta tcgcgggatg
     2101 atcgggcaca cgcagccaag gcgtctggcg gctcgagcgg tggccgcccg ggtcgcggag
     2161 gaactcggcg cccgggtcgg tgaggcggtg ggctacgctg tccggttcag cgatcaggtg
     2221 ggcgagcaca cgctggtgaa gctggtgacg gacggtctgc ttctcaacga gatacgtacg
     2281 gacaggtttc tcgacgccta cgaggtgatc atcgtcgacg aggcccacga gcgcagcctg
     2341 aacgtggact ttctgctcgg ctacctccgc aggctggtcg accgccgcca cgatctgagg
     2401 gtcatcatca ccagcgccac tatcgacgtg gcctctttct ctgagcattt cggcggggca
     2461 ccggtggtgg aggtcggcgg acgagggttt cctgtcgaga cccgttatgc agacgaggaa
     2521 gcgctggcgg atcgaagcgc cgaggagctg atcgtcgacc gtatccggtc catcgagaag
     2581 acgcccgtgg cgggatcagc gcgggacatc ctcgtgttcc agtccggcga gcgggagata
     2641 ttcgatactg cccacgcgct gcgccaagcg ctcgatgagc gttacgagat actgcctctg
     2701 tacgcgcgcc tgtcggcccg ggaccagcag cgcatcttca agccctcggg atatcgacgg
     2761 gtggtgatcg ccaccaatgt ggccgaaacg tcgttgacgg tgcccaacat cggcttcgtc
     2821 atcgatcccg gctttgcccg ggtgagccgc tactcgtttc gctccaagct gcaacggttg
     2881 cctgtcgagc ccatatcaca ggccagcgcg gatcagcgtc tgggccgctg cggccgggtg
     2941 gcgccgggcg tgtgctatcg gctttatacc gagaatgact atctgagccg gcctccatat
     3001 accgatccgg aaatcaagcg cacgaacctc gcccaggtcg tgcttcagat gcgctcgttg
     3061 gggttgggta aggtcgcggg ctttccgttt ctcgacccgc ccgacccccg ggcagtccgt
     3121 gacgcggagc gtctgctcca cgagctgggc gcgcttgaag ggggcggtct ttccaagacc
     3181 gggcgcatga tggcccggct cccggtggac ccgcgcctgg cccggatgat cattgcgggc
     3241 catgagcacc ggtcactgtc cgaggttctg gtgatcgtga gcgctctggc cgcccaggat
     3301 ccccgtgagc ggccgctgga acgccagggg tccgccgacc gggcccattc ggagttctcg
     3361 gatccgcgct cggattttct cgcctttctg aacatctggc agtggtacct ggaagcccgg
     3421 cagcgtcttt ctcgcgccag gctgtctcgc gagctcggct cgcgcttcat ttccgccacg
     3481 cggatgcggg agtggcatgc gctgcatcgg cagctgctgc tggcttgccg tgagcttgcg
     3541 ctcaaaccga acgatcagcc cgccgattac tcggccgtgc atcgcgcgct gctccaagga
     3601 tccttgagcc tggtaggtct gcacgaagag aagggcagct atctggggcc gcgtaacctg
     3661 cgattccgta tcttccccgg atcggcgctg gccaggcgtg ggccgaagtg gctgatggcc
     3721 ggagagatca ccgagacacg ccgggtttac gcgcgctgcg tggccggcgt cgaggctcga
     3781 tggatcgagg aagctgccgg cgatctggtc aagcgccgct actccgagcc gcgctggagc
     3841 gtacgaagag gcgaggtgat cgcgcttgag accgtcacgc tgtacggcct tcctttggcg
     3901 gacgggcgtc gcgtgagcta tcggaaggta gacccggatg cctgtcgtga gctcttcatt
     3961 accgaggcgc tcgtgcgcgg agggctcgaa gggcccttcg agttccttct ccacaatcgg
     4021 ggcctgattc gggagatcgt cgatgaggaa tccaagggcc gccggcgcga tttgctgatc
     4081 ggcgatgcgg ctcaggcgga tctttacgcg gccaggctcc cgcgggaagt gtgctcggtt
     4141 tcgacccttg gaaagtttct caagcaggcg gggcctgagg cgagggagcg tcttaaattc
     4201 tcgcgggaag agttgctcgg cgtggccagc gtcgagctgc ccgagaccga ttaccctggc
     4261 gcgttggaga ttcggggcgt ggcgttcccc ctgagttacc gattcgctcc gggagagcca
     4321 gacgacggcg tctccatcac ggttcccgtg ggcatgttga actcgctcgt cggcgaagcg
     4381 ctcgagtggg gcgtgccggg attcttcgat gtgctggtgg agcagtggct gcgatcgctg
     4441 cccaagacga agcgccggct tctggcgccg ctgcccgatc ggctcggtga catcgtcgcc
     4501 gtgctgtgcg ctgccgatcg atttcgccac ggccgcctgc tcccggcgct gggggaggtg
     4561 cttgccgaca acttcggttt gaaggtggct tcgacagatt gggatcgtgg tcgggtcgat
     4621 ccgcacctgc tcatgaacat caggatcgtc gatggcagcg gcaacctgct ggcccagagt
     4681 cgcgatctcg gcgagctgcg cagccggttc gccgcggaaa tcgaagagcg catggcatcc
     4741 tcggcggcgg cagagctgga gaacacgggg ctcacggcgt ttccggaagg cgggctggcg
     4801 gaatccgtca gcctgaagga tcgttccggc cagaccatcg cctatccagc cctgctcgac
     4861 gccggcgaca gcgtctcggt aaagctcttc gccgaggccc gggaacggga ccgtgccaac
     4921 agggacggct tcgcgaggct ggccctcatt gccgtggcgc agacgacaag gcacttgaag
     4981 aagggtctgg atgagcagcg tcgcctcggg ttgctttacg cggcgctcgg cggggccagg
     5041 caactggccg acgagttgct tcgtgcagcg gcgtggcgct gcttcttcgc cgatcggccg
     5101 ctgccgagat ccgaggccga tttcgagcaa cgcatccgga cgaatcgcgc cgacctggcc
     5161 gacactttct cggtcatgct cgaggttttc aaaacggttc tggagaaacg cttcgagatc
     5221 gtcacgctct gcgaaaccct aacctcaccg gcgttccgcg aggccgtggg cgatgtggcg
     5281 acgcagctcg acgagctcgt gcctgccgac gtcctctccc gggtgccccc tgcacggctt
     5341 gctgatcttc cgcgctatct cgacgctgcg cgctatcgcc tcgagcatct ccagggccgc
     5401 gtgaagcgcg atgaagagaa cacgcggctc gtcgcgagct tccgcgaccg gctgcgtcgc
     5461 ctcgaggcgg atcccgccgc caccgacggg gaagtcgccg agctgaggtt tgccgtggag
     5521 gagcttcgag catcgctttt cgcggaaccg ctggcggcgc gatgggccgc tggcggggat
     5581 cgttccagag tctctcccaa gcggttggac agatcgttcg aggccctcga gcgcgccctg
     5641 ggcctgatct gagacgtatg cctgggctgc gcgggccttt atactcgctc ggccggaagt
     5701 tgccggaacc gtaggggaaa gcgccggtca caagaaaaga gcgttcgtgg taagggcgct
     5761 cggaaccata acggaggggt cttcctagga cccgtgtgag gagaacttgt tgatgttcaa
     5821 tcgcaaaatg aagcccattg cggccgtgat aggaacggcg ttcgtcgcgt ctcttgcagc
     5881 caccagcgtt gccaacgcgg ccagcccgtt tgctgccaat gaactcgacc gcggttacct
     5941 gttgctggcc gacggccacg ccgagggaaa gtgcggcgaa ggcaagtgtg gcgaggataa
     6001 agatgccgag ggcgcgtgcg gcgaagataa agacgccgag ggtaagtgcg gtgaaggcaa
     6061 gtgtggtgaa ggcaagtgcg ggggcgctgc ctgagccgac gtcatcgctg acgtctgatg
     6121 tctagtccgg tgcatggcgc aggcctgggg ttgcgacgcg gtttgctcgc agaactcgcc
     6181 tccatggaga atgcatccgt tgatttcatg gagatcgcgc cggagaactg gataggggtt
     6241 ggcggccgct tcggccgcca gttccgagcg ctttgcgaac gtttccccat ggtctgtcat
     6301 gggctttccc tctccatcgg cggccccgcc ccgctggata tcgattttct caaagacatc
     6361 cgggtgttcc tcgacgagca tggcattgat tgctatacgg agcacttgag ctactgcagc
     6421 gacgacaagg gccatcttta cgatctgatg ccgatcccgt tcaccgcgga ggccgtccga
     6481 tacgtcgcgg ctcgcgtccg tcaggtgcag gacgtcctcg gccggcgcat cggcatcgag
     6541 aacgtctcct actatgtcgc tcctggccgg gaactgaccg agctggagtt catcaatgcg
     6601 gtgctggccg aggccgattg cgacctgctg ctggacgtca acaacatcta cgtcaactcc
     6661 atcaatttcg gttacgatcc cgtggacttc ctcaaccgaa tcgatggtgc gcggtccgtc
     6721 tacgtgcacg tcgccggcca ctacgtggaa gcgcccgatc ttcgggtcga cactcacggc
     6781 agcgccgtcg ccgagccggt ctgggacctg ctttcggctg cctacaaccg ctggggcgcg
     6841 ctcccgacgc tgctcgagcg cgacttcaac tttccgcccc tgggtgaact tctccatgag
     6901 gtgtcgagaa ttcgctcgct gcaggcggcg gtacggtctg gccatgccgc gaacgggtga
     6961 aaacctcccg gaatttcgcg ctcggcaact cgatttcgcc gcgcatctgc gcaaccctga
     7021 tctgtatccc tgcccggaag gtctcgatgc gcgccggatg cggatctacg tggatctggt
     7081 ctacaacaac gtcgagagct tcctggccaa tggctttccc gtcgccaagg agattctggg
     7141 cgatgcttcc tggcacgcgc tgattcgtga cttccttcac cggcatccca gcgagtcacc
     7201 atactttctg gagatcccgc aggagtttct cgccttcgtc agttcctgcg accgttcgga
     7261 ggttcccgac tactttctcg agctttgcca ttacgagtgg gtcgagttgg ctctcgccac
     7321 ggcggaggat gatccgccgg aagccggcgt agatccttcg ggagatctcg tgcatggtcg
     7381 gccggtcgtg tcgccgctga tctggccgct tgcctaccga tatccggtgc atcgcatcgg
     7441 tcccggattt cgtcctgacg caccccctga atcgcccacg cacctgatcg tgttccgccg
     7501 ccgtgatgat tccgtgggat tcctcgaggc gagcgcagcc acccttaggc tggtagagct
     7561 gctcagtgag ggcgccacgg gcgcatctgc cattgagcgg ctgtgtcagg agatgcccgg
     7621 cttggactca caagtcgttc acgacgaagg ggttgcaacc atgatgcgcc tgcgtgaggc
     7681 tggcatcata ctgggcaccg agattgcagg tgcgagtgag tgacggcaga gcccgagggc
     7741 gagccaggtt taccaccatc cttctctgcg cgctgacacc gctggtcacc cccatgtccg
     7801 gcgccgccca ggtcaatccg ggcgaagccg ccaaccgcgg ggtgtacggc gtgaacgatc
     7861 gggccgaccg cgcgttgttg cgtcccgttg ccaagggtta taccaaggcc gttcccggct
     7921 tcctgcgccg cgcggtgcac aacgttctgc tcaacctggg aactcccctg gtagccgtca
     7981 accagctgct gcaggggaag ccgaacgagg ctttcagcga ttcgggccgc ttcctcgtga
     8041 acagcaccgt cgggatcgct gggatcttcg accccgcaac caagctcggt atcgaagagc
     8101 atacggagga cttcggtcag acgctcgcgg tatggggggt gccgtcgggg ccttacatgg
     8161 tggtgccgtt ctgggggccc agctacctgc ggcacgcctt cggcatgatc gtggagttct
     8221 ttgccagccc gttgcgtctg atttctccgg ctcgagaccg ctacatcgcc tatggcgtca
     8281 ccatcctcga ccggcgtgcc cggctcattg ggcccgacga gatgattgcg ggagacgact
     8341 atctgttcgt ccgcgaggcg taccagcaaa ggcgtgaata tctgatcctg gatggtgccg
     8401 tggaggaaga ccctttcatg atggatgaag catggcagga ggagccgtga agagctcccg
     8461 ggttcgctgc caagccgcgt tgcatttgac caatgatagg agtacggtgc gcgtcccacc
     8521 gatgcggcac ccgtttagct tgctatgagc ctcgtcgttg cagaccctga tgaagaggct
     8581 ctggcgaact tgcgaactca gctggaccga ctgggtctcg acccgattcc tgcagccgat
     8641 ctcaagtttg cctctgagat ctgcgagcac tcgccccccg atctcgtgat cagcggagac
     8701 ctcgattggg ccgagcggct cgccgatatg caccgacggc ttccggtggt tctcgccgtg
     8761 gaccgcaacc ccgaaaggga cgtgctcatc cgggcgctgc gctgcggact ggccgacgtg
     8821 tggacactgc cggcagagga cgacttcctc cgaggacggg tggacgacat tctccgcaac
     8881 aacgaagcgg ccgcatctca ggcggagaaa aggttggggc agtacgtagc cactttgcag
     8941 cgtgaccagc gggccggccg ctacatccag atgggaatgc tgcccccgtc acccatggca
     9001 atcgatcgtt ttcgatttca gcaccgcatc gttccgtccc tgatgttgag tggtgatttc
     9061 gtcgactact tccgcctcac cgatcggcac ttcgtcttct acattgccga cgtctccggg
     9121 cacggcgcat cgtcggcgtt cgtgaccgtg ctgctcaaga acttctcccg caggctccgt
     9181 cgtgagtatc ggcccagcat gctccaggcg cccggcgaga tcctcgagtg gttcaatcgg
     9241 gagctcctgg agcagcagat cgacaagcac gtcgccatga ttctcggcct gggcgacctg
     9301 gaaaagaacg tggtcagtct ggtcaatgcg ggccattttc cacccgccat ccacgtcgcc
     9361 ggacagtcgc acgccgttta tgtcgagcag cggggtaagc ccgttgggct cttcgagacg
     9421 gtcagctacc
//
LOCUS       DBNHKV010000022         3597 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_286732, whole genome shotgun sequence.
ACCESSION   DBNHKV010000022 DBNHKV010000000
VERSION     DBNHKV010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_286732"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..2743)
                     /gene="pepN"
                     /locus_tag="AC16PP_00600"
     CDS             complement(71..2743)
                     /gene="pepN"
                     /locus_tag="AC16PP_00600"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HIR6480148.1"
                     /translation="MSSNVPQPVFLRDYEPPSYRTLDTRLSFDVRDGYTRVESQLSVE
                     RRAGGTDTVLRLDGQNLELLSVALDGRLLGGNEFVVDAESLTLFDLPERCEITIVTRI
                     EPEKNTALEGLYKSGSMYCTQCEAEGFRRITYYQDRPDVLARFTTTLEADASLYPVLL
                     ANGNPISDVPAGEGRRRVTWEDPFPKPSYLFALVAGDLAVMEDEFVTASGRRVALRIF
                     SEPQNIGQCGYAMEALKRAMRWDEETFGREYDLDILMIVAVEDFNMGAMENKGLNIFN
                     TKCVLASPDTATDAAHLRVESVVAHEYFHNWSGNRVTCRDWFQLSLKEGFTVFRDEEF
                     SSDMNFRAVKRIEDVNFLRSSQFPEDAGPMAHPVRPDSYIEISNFYTTTVYEKGSEVV
                     RMLRTLLGEERFRRGSDLFFDRYDGCAVTTDDFLGAMADVTALDLKQFRRWYEQAGTP
                     RLQVEEGWHDGELNLRIRQSCPPTPGQGSKKPFHLPVLIGLLDGAGRDLVDGGLDCSS
                     DAQVDVREGDRSLLVHVRDEITEIRIPGLPGRPAVSFLRGFSAPVRVEYPRDPETLAF
                     LALHDSDGFARWDALQTLVVREIDAMRSGGEPAAELVALLGNFIDPALAGGASAEQRF
                     LLSELLHVADEGYLFQALDDFEVEAVCDARDRLVVSVAANHAERWRALYEANAASGAY
                     APEPDGMSRRALRNTALGYLARVETGSALESLLEDHYRSADNLTDRSAALRAVADVDG
                     LSDSFRSEMLDEFHRRWRAHALIVDLWFSVQASSPRSSAGTIRELESHPDFDGLNPNK
                     LRALHGAFGQMNHRNFHAQDGSGYDLLADRVLELDRNNPQIAARLLTPLTRWRRYDTH
                     RQALMTAALRRIEDQEGLSKDVFEVVTKTLATED"
     gene            complement(2785..>3597)
                     /locus_tag="AC16PP_00605"
     CDS             complement(2785..>3597)
                     /locus_tag="AC16PP_00605"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR6480149.1"
                     /translation="VLSRLLWERRVRHRIYEERGRQIVELADGAQAALVRDVYEALAS
                     GSLVVEAQPLRQRRPGLLAQIRRYPGLAVLVALAVALFPFTMPLAEGRVTYVASWLTI
                     VDLIRLGSAPGTLVELVAEAAVWRWFAPMLIHFNAVHLLFNCAVVIEMGRRIESRLGA
                     LWFWAIVLVVSLVANLGQYAMNANPLFGGLSGVAFGLLGFVLAAGRLAPAVPQWQVPT
                     AVAGGLLFFLVLFSTGVTEYFGLHVANAAHWFGLAAGAVLGIVTGRLVGSGR"
BASE COUNT          700 a         1197 c         1138 g          562 t
ORIGIN      
        1 caagggacgg cgcaaaccct ccgggcccga agcgcttccc gagctgtacg cctgaccggc
       61 ccggggcagg tcagtcttcg gtcgccagcg tcttggtgac cacctcgaat acgtccttgg
      121 acagcccttc ctgatcctcg atcctgcgca atgccgcagt catcagcgcc tgacggtgag
      181 tgtcgtagcg tcgccagcgc gtgagcggcg tcagcagccg cgcggcgatc tgcgggttgt
      241 tccggtcgag ttccagcacc cgatccgcca ggaggtcgta gccggatccg tcctgggcat
      301 ggaaatttcg atggttcatc tggccaaacg ccccatgcaa agcgcgcagc ttgttgggat
      361 tcaggccatc gaagtcaggg tgcgattcaa gctccctgat ggtgccggcg ctgctgcgcg
      421 gactgctcgc ctgaaccgaa aaccacaggt cgacgatgag cgcgtgtgcc cgccagcgcc
      481 gatgaaactc gtcgagcatt tcactgcgaa aagaatcgct gagaccatcg acgtcggcga
      541 cggcccggag ggcggcgctg cggtccgtga ggttgtcggc cgaccggtaa tgatcctcga
      601 gcaacgactc cagagcggac ccggtctcga cccgggcaag atacccgagg gccgtgttgc
      661 gcagggcccg gcgggacatg ccgtcgggct cgggcgcgta ggcacccgag gccgcgttgg
      721 cctcgtaaag cgcgcgccac cgctcggcgt gattggccgc caccgacacc acgagccggt
      781 ctcgggcatc gcaaacggct tctacctcga agtcgtcgag cgcctggaag agataaccct
      841 cgtccgcgac gtgcagtaac tccgacagca gaaagcgctg ctcggcgctg gccccaccgg
      901 ccagcgcagg atcgatgaag ttgcccagca gcgctacgag ctcggcggcg ggttcgccgc
      961 cggagcgcat ggcgtcgatc tccctgacca ccagcgtctg cagcgcatcc cagcgcgcaa
     1021 aaccatcgga gtcgtgaaga gccagaaagg caagcgtctc ggggtcacgg ggatattcca
     1081 ctcgaaccgg agcggagaac ccccgcagga aactcaccgc cggacgcccg ggcagaccgg
     1141 ggatgcgaat ctcggtgatc tcatccctta cgtgaacgag cagcgatcgg tcgccctcgc
     1201 gcacatcgac ctgggcgtcg ctcgagcagt ccaggccccc atcgaccagg tcgcggccgg
     1261 caccatcgag cagcccgatc agaacgggaa ggtggaaggg cttcttgctg ccctgcccgg
     1321 gcgtcggcgg gcaggactgc cggatgcgca ggttcagctc gccatcgtgc cagccctcct
     1381 ccacctgcaa tcgtggcgtc ccggcctgct cgtaccagcg ccggaactgt ttcagatcga
     1441 gggcggtcac gtccgccatg gcgcccagaa agtcgtcggt cgttaccgca cagccgtcgt
     1501 agcgatcgaa gaagaggtcc gaaccacggc ggaaccgttc ctctccgagg agggtccgca
     1561 gcattcggac cacctcgctg cccttctcgt agaccgtcgt ggtgtagaag ttggagatct
     1621 cgatgtaaga gtcgggccgc accgggtgcg ccatggggcc ggcgtcctcg ggaaactgcg
     1681 acgaacgcag aaaattcacg tcctcgatgc gcttgaccgc ccggaagttc atgtcggagg
     1741 aaaactcctc gtcccggaaa acggtgaatc cttccttcaa gctcagctga aaccagtcgc
     1801 gacaggtgac gcggttgccg gaccagttgt gaaaatattc gtgagccacg acgctctcga
     1861 cacgcagatg ggcggcatcc gtggcggtgt ccggcgaagc cagaacgcat ttggtgttga
     1921 agatgttcag gcccttgttc tccatggcgc ccatgttgaa atcttccacc gccacgatca
     1981 tgaggatgtc gagatcgtac tccctgccga acgtctcctc gtcccagcgc atggcgcgct
     2041 tgagcgcttc catggcgtaa ccgcactggc cgatgttctg tggctccgag aagatccgaa
     2101 gcgccacgcg acgtccggaa gcggtcacga actcgtcttc catgaccgcg aggtcgccgg
     2161 ccacgagcgc aaagagatag ctcggcttgg gaaacggatc ctcccaggtc acccggcggc
     2221 gcccttcgcc cgccgggacg tcggagatgg gatttccgtt ggcgagcagg accgggtaga
     2281 gcgatgcgtc tgcttccagg gtcgtggtga agcgcgcgag aacgtcggga cgatcctgat
     2341 agtaagtgat ccggcggaac ccctcggctt cgcattgggt gcagtacatg ctccccgatt
     2401 tgtatagacc ttccaacgcc gtgttctttt ccggctcgat acgggtcacg atcgtgatct
     2461 cgcagcgctc cggcaggtcg aagagggtca gcgactccgc atcgaccacg aactcgttgc
     2521 cacccagcaa ccggccatcc agggccaccg agagcagctc caggttctga ccatccagcc
     2581 gtagcaccgt gtccgtgccg cctgcgcggc gctccacgct cagctggctc tcgacgcgcg
     2641 tgtagccatc gcgtacgtcg aagctcagcc gggtatccag ggtccggtaa gacggcggct
     2701 cgtagtcccg gaggaacacg ggttgaggga cgttgctact catcggcaaa gctccatatg
     2761 caaccgttga cggcctcgcg ccgatcagcg accggagccg accagacgac ccgtgacgat
     2821 gccgagaact gctccggcgg cgagtccgaa ccaatgggcc gcgttggcca cgtgaagacc
     2881 gaaatactcg gtcacgcccg tgctgaacag caccaggaag aacagcagcc cacccgcgac
     2941 agcggttggc acctgccatt gaggcacggc gggcgccaga cggccggccg ccaggacaaa
     3001 gcccaacagg ccgaaggcga cgccggaaag cccgccgaac aggggattcg cgttcatcgc
     3061 gtactgcccg agattggcca cgagcgaaac caccaggacg attgcccaga accagagtgc
     3121 cccaagccga ctttcgatcc gccggcccat ctcgatgacg acagcgcagt tgaacagaag
     3181 atgcacggcg ttgaagtgga tcagcatggg cgcgaaccag cgccagaccg cggcctccgc
     3241 caccagctcg accagcgtcc cgggagcgga tccaagccga atcagatcca cgatcgtcag
     3301 ccagctggca acatacgtta cgcggccttc ggccagcggc atggtaaacg gaaacagcgc
     3361 cacggccagg gcgaccagga ccgcaagccc cgggtacctg cggatctgcg cgagcagccc
     3421 cggacggcgc tgccgcagag gctgtgcctc gacgaccaga gaacccgaag ccagcgcctc
     3481 gtagacgtcc cgaaccaatg cggcctgggc accgtccgcc agctcgacga tctggcgccc
     3541 gcgttcctcg tagatgcgat gacgcaccct gcgttcccag agcaggcggg acagcac
//
LOCUS       DBNHKV010000023         2409 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_144910, whole genome shotgun sequence.
ACCESSION   DBNHKV010000023 DBNHKV010000000
VERSION     DBNHKV010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_144910"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1724)
                     /locus_tag="AC16PP_00610"
     CDS             complement(114..1724)
                     /locus_tag="AC16PP_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6480150.1"
                     /translation="MLLDLEKLPQGERFQADVCIAGAGAAGVALARRLAELGHSICLL
                     ESGGLDFEQSTQDLYAGTSDGMTYYDLVDARLRFLGGTTNIWGGRCALLDDIDFRRRP
                     WVPHSGWPITNAELTPYYAAVHDELELGEVRYDESLWDEVGERVPPFDPDAVSTRFWR
                     FDDVRERFAARRVQDLASSAAVTLVLHANVTHIQAAANAASIEHLKVSSLSGIEGRVT
                     ARHYVLACGGIENARLLLASRDVEAAGVGNGNDQVGRYFMEHPHGRAGRVSGPGAFPL
                     WAAFRKRFRSHGPDLAPVLVPSAAAQEREGILNSAFTLSLQRDPERGVAVTRQMYQTL
                     KHGMAPTRRGRTLWHAYRAVRSSAQRLARVPFERLRAGLGMRRLYLMIRAEQAPNPDS
                     RVTLGSERDALGMPRAALSWQLGELDKRTIQVMVRLLDGELTRLGLGSVEASSWLGEP
                     GNDWPVDPTVGNHPIGGYHHIGTTRMSADPRHGVVDGSGRVHGYGNLFVAGSSVFATA
                     GWANPTLTILALALRLGDHLHGLLGGKS"
     gene            complement(1727..>2409)
                     /locus_tag="AC16PP_00615"
     CDS             complement(1727..>2409)
                     /locus_tag="AC16PP_00615"
                     /inference="COORDINATES: protein motif:HMM:NF016974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HIR6480151.1"
                     /translation="RAIGRFQGRHSLVGTGPFLENAEFPWVPSLEGRWEEIRSELATV
                     MEHPEDIPAFHQLSPDQKRISKGDNWKTFAFYVFGIRLEENCRRCPVTAATIESLPGL
                     QNAWFSILAPRYHIPPHKGPTRAVIRCHLGLAVPRDGERCWLRVDDQIRHWEAGGCLL
                     FDDTFEHEVRNDTDETRIVLFLDVDRPSDRLGTWFNRLIVTLIRGSAYVKDPLKNLAD
                     WNRARREA"
BASE COUNT          410 a          828 c          808 g          363 t
ORIGIN      
        1 tcccggccgt cagcggcagc agcctgcggc cctgaccagc gctcagaatg atggccttca
       61 ctagggtacc ggtatcggga aaacgcgaag ggtcggggtt ttcaccgcga gggtcaagac
      121 tttccgccca gcaggccgtg cagatggtct cccagccgca gcgccagggc cagaatcgtc
      181 agggtggggt tcgcccagcc ggcggtggca aagacggagc tgccggcgac gaacagattg
      241 ccgtagccgt gaacgcgccc actcccgtcc accacaccgt gccgaggatc ggcactcatg
      301 cgagtggtgc cgatgtggtg gtacccgccg atggggtggt tgcccacggt cgggtcaacg
      361 ggccagtcat tgcctggctc gcccagccag gaactcgcct cgacggaacc caggcccagc
      421 cgggtcagct cgccgtccag cagccgcacc atcacctgga tcgtgcgctt gtccagctcg
      481 ccgagctgcc agctcagggc agcccggggc atgcccaggg cgtcacgctc gctgcccagg
      541 gtgacgcgac tgtccgggtt cggggcctgc tcggcccgga tcatgagata aaggcggcgc
      601 atgccgaggc cggcccgcag ccgctcgaag ggaacccggg caagccgctg ggccgagctg
      661 cgcacggccc tgtaggcgtg ccacagggtc cgcccccgcc gggtcggcgc catgccgtgc
      721 ttcagggtct gatacatctg ccgcgttacc gcgaccccgc gctccggatc ccgctgcaga
      781 ctgagcgtga acgcagaatt gagtatcccc tcgcgctcct gagcggccgc agacgggacg
      841 agaacgggcg ccagatccgg tccgtgactg cgaaagcgct tgcggaaggc cgcccacaag
      901 gggaacgcgc ccggccccga gacccggccc gcgcggccgt gcgggtgctc catgaagtag
      961 cgcccgacct ggtcgttgcc gttgccgacc ccggccgcct ccacatcgcg ggaggccagc
     1021 aggagacggg cattttcgat cccgccgcag gcgagcacgt agtgccgcgc cgtcacccgc
     1081 ccctcaatcc cggaaagcga agacactttc agatgctcga tgctggccgc gttggccgcc
     1141 gcctgaatgt gggtcacgtt ggcgtgcaga accagagtca cggcagcgct gctggccagg
     1201 tcctgcaccc gacgggcggc gaagcgttcc cgcacgtcgt cgaagcgcca gaatcgcgtg
     1261 ctgaccgcgt ccgggtcgaa gggcggcacc cgttcgccga cctcgtccca caacgattcg
     1321 tcgtaacgca cctcgccgag ctcgagctcg tcgtgcacgg ccgcgtagta aggcgtgagc
     1381 tccgcattgg tgatgggcca gccgctgtgc ggcacccacg gcctgcgccg gaaatcgatg
     1441 tcgtcgagca aggcgcagcg cccgccccag atgttggtgg tgccccccag aaagcgcagg
     1501 cgggcatcga ccaggtcata gtaggtcatg ccgtcgctgg tcccggcgta gagatcctgg
     1561 gtggactgct cgaaatccag gccgccgctc tccagcaggc agatggagtg accgagctcc
     1621 gccaggcgcc gggccagcgc cacaccggct gcgccggcgc cggcaatgca gacgtcggcc
     1681 tggaaacgct cgccctgggg cagcttttcg agatcgagca gcacgactaa gcctcgcgcc
     1741 tcgcccggtt ccagtcggcc aggttcttca gcggatcctt cacgtaggcg ctgccgcgga
     1801 tgagcgtgac gatcagtcgg ttgaaccacg tgcccaggcg gtccgacggc cggtccacat
     1861 ccagaaacag cacgatgcgc gtctcgtcgg tatcgtttcg gacctcgtgc tcgaacgtat
     1921 catcgaaaag cagacagccg cccgcctccc agtggcggat ctggtcgtcg acgcgcagcc
     1981 agcaccgttc tccgtcacgg ggcacggcaa gacccaggtg gcaacgaatc accgcacggg
     2041 taggcccctt gtgcggcggg atgtggtagc ggggcgcgag gatggagaac caggcgttct
     2101 gcagacccgg aaggctttcg atggtcgcag ccgtgaccgg gcatcgccgg cagttttctt
     2161 ccaggcggat accgaacacg tagaaggcga acgtcttcca gttgtcgccc ttggagatgc
     2221 gcttctggtc cggcgacagc tgatgaaacg cggggatgtc ctccgggtgt tccatgaccg
     2281 tagccagctc ggatcggatc tcctcccagc gcccctccag cgagggtacc cagggaaatt
     2341 cggcattctc cagaaacggt cccgtgccca ccagagagtg gcggccctgg aaccggccga
     2401 tggcccgca
//
LOCUS       DBNHKV010000024         3996 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1065881, whole genome shotgun sequence.
ACCESSION   DBNHKV010000024 DBNHKV010000000
VERSION     DBNHKV010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1065881"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="AC16PP_00620"
     CDS             complement(<1..467)
                     /locus_tag="AC16PP_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6480152.1"
                     /translation="MHPGIIAAANPDKVAYLMADSGETITYGELEKTSNQGAHLFRQL
                     GLKRGDHIALLLENHPRFFQICWAAQRAGLYYTAISWRLQQAEVEYIVNNCEAAVFIT
                     SAARREVVAPLADSMPGVSHRFVIDGAIEGFDDWAEAISAMPVTPVADESEGMA"
     gene            610..2121
                     /locus_tag="AC16PP_00625"
     CDS             610..2121
                     /locus_tag="AC16PP_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR6480153.1"
                     /translation="MASSQPLAVQAGLDAMRSGGNAVDAALATAIALTVVEPNNNGVG
                     SDAFAIVWDGSKLVGLNASGRSPAGWTPDRFAGQHTMPELGWDAVTVPGAVSAWSALS
                     ERYGRLAFPDLFDAAIRYAAEGFPVGPKTAYYWQFAERRYADFPDFANHFLPAPRAGE
                     RFRRPDLARTLQEIAATGGKAFYEGDIAERIAAAAATDGGVMTRDDLAAHRAEWVDPL
                     SQPYRSVDVHEIPPNGQGLAAQIALGIFAHFDAPPLETAESVHLQVEAMKIAIRAAAD
                     HFADADAMRLPPAELLDPANLRRAAGTIGRSASTLPPVSLPTSQDTVYLCAADASGMM
                     VSFIQSNFMGFGSGIVIPGTGISMQNRGSGFILEPGHPNRVGPRKRPYHTIIPGFVTE
                     NGRPRLAFGVMGGHMQHQGHFQMVQRIFDHGQNPQAASDAPRWRVFPDYSLRVEPEFP
                     EKVIKELADRGHQIGVEDQEHQFGGAQLIYRNESEDAYIAGSDHRKEGMAAGF"
     gene            complement(2127..2534)
                     /gene="msrB"
                     /locus_tag="AC16PP_00630"
     CDS             complement(2127..2534)
                     /gene="msrB"
                     /locus_tag="AC16PP_00630"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR6480154.1"
                     /translation="MGKVTKSDDEWRSELDTHTYEIARKAGTERAFTGKYWDTKTPGI
                     YHCTCCGASLFDSDTKYDSGSGWPSFYEPVSHDAVSTREDNSLMMQRIEVLCAECDAH
                     LGHLFPDGPEPTGLRFCMNSASLDLQERDEDGS"
     gene            2645..3931
                     /locus_tag="AC16PP_00635"
     CDS             2645..3931
                     /locus_tag="AC16PP_00635"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR6480155.1"
                     /translation="MDSAENDKVTPLPASAAVVTGEAGRRQILKSHKLDDVCYDIRGP
                     VLEEAHRLEEEGHRVLKLNIGNPAPFGFEAPEEILLDVIHNLPESQGYSDSKGIYSAR
                     KAIMQYCQQQGVPNVEIDDIFLGNGVSELVVMSLQGLLNNGDEVLIPAPDYPLWTAAT
                     HLSGGRAVHYRCDEQSDWNPDLADIKARITDRTKAIVIVNPNNPTGAVYDRSLLVELV
                     ELARANDLVIFADEIYDKILYDDAEHVPLATLAEDLLVVTMNGLSKTYRVAGFRSGWM
                     VVSGAKHRAQDYMEGLNILASMRLCANVPAQHAIQTALGGYQSIQQYLVPGGRLYEQR
                     SVAWEMLNAIDGVSCVKPKGALYLFPKLDVRKFNITSDERFVLDLLRQERILVVQGTG
                     FNWFEPDHFRIVFLPHEEDLRHALERLEHFLAGYRQ"
BASE COUNT          754 a         1329 c         1208 g          705 t
ORIGIN      
        1 gccatccctt cgctttcgtc ggcaacgggg gtgaccggca tggcggagat cgcctcggcc
       61 cagtcgtcga aaccctcgat ggctccgtcg atgacgaacc gatgggagac acccggcata
      121 ctgtccgcca gcggcgcaac cacctcgcgc cgtgcagccg aggtgatgaa cacggccgcc
      181 tcgcaattgt tgacgatgta ttccacttcc gcctgctgca ggcgccagct tatggccgtg
      241 tagtaaaggc ccgcacgctg ggccgcccag cagatctgaa agaaccgcgg gtggttttcc
      301 agcagcaacg cgatgtgatc gccccttttc agcccgagct gtcgaaacag gtgcgcgccc
      361 tgattcgaag tcttctccag ctcgccgtaa gtgatggtct cgcccgagtc ggccatgagg
      421 taggcaacct tgtctgggtt ggccgccgcg atgattcccg gatgcacgct gtctcccttg
      481 gattgcgccc gatatgcagg cagccaaagt taggggcttt tcccgattca cgcaacgcag
      541 taacgtctgc tgatggattg ggaacagccg tatcgggccg ctcgctcggc cgtttgcgcc
      601 gagaacatcg tggccagctc tcaaccgctg gccgtgcagg ccggcctcga cgccatgcgc
      661 agcggcggca acgcggtgga cgcggccctg gcaaccgcca tcgcgctgac cgtggtggaa
      721 cccaacaaca acggcgtagg cagcgacgcg ttcgccatcg tgtgggacgg ttcaaaactc
      781 gtcggcctga acgcttcggg ccgctctccc gccggctgga cgcccgaccg cttcgccggc
      841 cagcacacaa tgcccgagct cggatgggac gccgtgacgg tcccgggcgc ggtcagcgcc
      901 tggtcggccc tgtcggagcg ctacggccgg ctcgcgtttc ccgatctgtt cgacgccgcc
      961 atccgctatg ccgcggaagg gtttcccgtt ggccccaaaa cggcctacta ctggcagttt
     1021 gccgagcgcc gctacgcgga ctttccggat ttcgccaatc acttcctgcc cgcgcctcgc
     1081 gccggtgagc ggtttcggcg cccggatctc gccagaacgc ttcaggaaat cgcagccacg
     1141 ggcgggaaag ccttctacga aggcgatatc gctgagcgaa tcgcggccgc ggccgccacc
     1201 gacggtgggg tcatgacccg ggatgacctg gctgcgcatc gcgccgagtg ggtggacccg
     1261 ctctcccagc cttaccgcag cgtcgacgtc cacgagattc cacccaacgg ccagggcctt
     1321 gccgctcaga tcgccctggg catctttgcc catttcgacg cgcccccgct cgaaacggcc
     1381 gaaagcgtcc acctccaggt cgaagccatg aagattgcga tccgggcggc ggccgatcac
     1441 tttgccgacg ccgacgccat gcgcctgccg ccggcagaac tgctggaccc ggcaaatctg
     1501 cgccgcgccg ccgggaccat cggcaggagc gcgtcgacgc tcccgcccgt gtcgctccca
     1561 accagccagg ataccgtcta cctctgcgcc gccgatgcga gcggcatgat ggtctcgttc
     1621 atccaatcca acttcatggg gttcggatcc ggcatcgtga ttcccggcac cggcatttcc
     1681 atgcagaacc gcggatcagg gttcatcctg gaacccgggc acccgaaccg tgtaggcccc
     1741 cgaaaacgcc cgtaccacac catcatcccg ggattcgtga ccgaaaacgg gcgcccccgg
     1801 ctggcgttcg gcgtcatggg cggccacatg cagcatcagg gacacttcca gatggtgcag
     1861 cgaatattcg atcacggtca aaacccccag gccgccagcg acgctccccg ctggcgggtc
     1921 tttcccgact acagcctgcg ggtggagccg gaattcccgg agaaagtcat caaagagctg
     1981 gccgaccgcg gccatcagat cggtgtcgaa gatcaagaac accagttcgg cggtgctcag
     2041 ctcatctacc gaaacgagtc ggaagacgcc tacattgcgg ggtcagacca tcgcaaggaa
     2101 ggcatggccg ccggcttctg acgaggtcac gacccgtcct cgtcgcgttc ctgaagatcg
     2161 agcgaggcgg agttcatgca gaagcgcaat ccggtcggct cgggaccgtc cggaaatagg
     2221 tgacccaggt gggcgtcgca ttcggcacag agcacctcga tgcgctgcat catcaggctg
     2281 ttgtcctcgc gcgtgctcac cgcatcgtgg gagacgggtt cgtaaaagct gggccaaccc
     2341 gagccagagt cgtacttggt gtcggaatcg aacagagagg cgccgcagca ggtgcaatga
     2401 tagatacccg gcgtcttggt atcccagtac tttccggtaa acgcccgttc ggtgccggcc
     2461 ttccgggcga tctcgtacgt gtgcgtgtcc agctccgagc gccactcgtc gtctgatttg
     2521 gtcaccttac ccatatctgg ttcccctgct accatgccac cagtttacaa tcgccgccgc
     2581 caacatcgca gcgcgcgaaa ttctatgcct ggaaggcagc cgatggaaac ctggatcccc
     2641 aacgatggat agcgcagaga acgacaaagt cacgccgcta ccggcgtcgg ccgcggtcgt
     2701 cacgggtgaa gcggggcgcc ggcagattct caagtcgcac aagctcgacg acgtctgcta
     2761 cgacattcgc gggcccgtgc tggaggaagc gcacaggctg gaggaggaag gccatcgggt
     2821 cctcaagctc aatatcggca acccggcacc gttcggcttc gaggcgcccg aggaaattct
     2881 tctcgacgtc attcacaacc ttcctgaatc ccagggctat tcggactcga aaggcatcta
     2941 ctcggcgcgc aaggccatca tgcagtactg ccagcaacag ggcgtgccca acgtggagat
     3001 cgacgacatc tttctcggca acggggtgtc cgagctcgtg gtcatgtcgc tgcagggcct
     3061 gctcaacaac ggcgacgagg tgctcatccc cgcgcccgac tacccgttgt ggaccgcagc
     3121 aacacacctc agcggcgggc gggccgtgca ctatcgttgc gacgagcagt ccgattggaa
     3181 cccggacctg gcagacatca aggcacgcat caccgaccgg acaaaggcca tcgtgatcgt
     3241 gaatccgaac aaccctaccg gagccgttta cgacagaagt cttctggtcg agctggtgga
     3301 gctggctcga gccaacgatc tggtcatctt cgccgacgag atctacgaca agatcctcta
     3361 cgacgatgcc gagcacgttc cgctggccac gctggcagaa gacctgctcg tggtgaccat
     3421 gaacggtctg tcgaaaacct atcgggtcgc cgggttccgc agcggctgga tggtcgtcag
     3481 cggtgccaag caccgcgctc aggactacat ggaagggctg aatatcctgg catccatgcg
     3541 cctgtgtgcg aacgtccccg cccagcacgc catccagacc gccctgggcg gctatcagag
     3601 catccagcag tacctcgtcc cagggggacg cctttacgag cagcgcagcg tcgcatggga
     3661 gatgttgaat gccatcgacg gggtgagctg cgtcaagccc aagggggccc tttacctgtt
     3721 cccgaagctg gacgtccgca agttcaacat caccagtgac gagcgctttg tgctcgacct
     3781 gctgcgtcag gaaaggatcc tcgtcgtgca gggcaccggc ttcaactggt tcgagcccga
     3841 ccatttccgc atcgtgtttc ttccccatga ggaggacctg cgccacgcgc tggagcgcct
     3901 cgagcacttc cttgccggct atcggcagtg agcgacatca acatcgatga ttttttcaag
     3961 gacgccgcca tcgccctggt gcagctctac ggcatg
//
LOCUS       DBNHKV010000025        13728 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_499964, whole genome shotgun sequence.
ACCESSION   DBNHKV010000025 DBNHKV010000000
VERSION     DBNHKV010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13728
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_499964"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="AC16PP_00640"
     CDS             complement(<1..231)
                     /locus_tag="AC16PP_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003296664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR6480156.1"
                     /translation="MSDSFADLFEESLTTIEMEPGSIVTGTVVDIDNDWVIVHAGLKS
                     EGVIPRNQFINEEGEFTLSVGDQVQVAMEVVDD"
     gene            complement(310..984)
                     /gene="cmk"
                     /locus_tag="AC16PP_00645"
     CDS             complement(310..984)
                     /gene="cmk"
                     /locus_tag="AC16PP_00645"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR6480157.1"
                     /translation="MSLPPVITVDGPSGSGKGTLAGLLAERLGWHLLDSGALYRIVGA
                     QAIARGLALDDEAAIAGMAAGLDIRFEDDRVLVDGEDLTRTIRTEAVSSAASQVAALE
                     GVRQAIFGLQRAMRRAPGLVADGRDMGTVVFPEADLKVYLEASPEVRAERRYNQLKNK
                     GLSVTFRALLASIRERDERDMRRAVSPLRPAQDAILVDSTELTIDQVLDRVLAEARTR
                     GLAKGI"
     gene            complement(1018..1839)
                     /locus_tag="AC16PP_00650"
     CDS             complement(1018..1839)
                     /locus_tag="AC16PP_00650"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR6480158.1"
                     /translation="MLLTVVGTGLIGGSFALGARTRNLFQTFVGVDPDPGNAQSALSL
                     GIVDEIADEVPAASDAVLIAAPSDAVAEWVVRLADHPGILFDAGSVKGAILDAIRGQL
                     GEIPERFVPCHPIAGREVSGPGAANEDLFAERLVVQTPVAGTVPEAREQVAYWWRSLG
                     ARVESMDPELHDRVYALTSHLPHLIAFAYMQQIEPAHLDHAGGGFRDFSRIGGSDPDM
                     WSAIFGLNKDAVLAALAGFEADLADLRRAIEAEDEDAVRDCIARASERRRGYHGD"
     gene            complement(1844..2938)
                     /gene="hisC"
                     /locus_tag="AC16PP_00655"
     CDS             complement(1844..2938)
                     /gene="hisC"
                     /locus_tag="AC16PP_00655"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR6480159.1"
                     /translation="MSPRVNPRIKALKPYQPGKPVEEVTRELGLVDVIKLASNENPRG
                     PGELVRQAIDAACGDLARYPDGNAFALKQALARHFGVRESQLTVGNGSNDVLDLAARM
                     SLSPGAEAVISEHAFVVYRLATLSAGGELVEVPARNYGADLDGILDAITDATAIVFLA
                     NPNNPTGTWVGEGALVRFLDEVPEHVWVVLDEAYHEYVEAAGYPNGMELQRRYPNLIV
                     TRTFSKIHGLAALRVGFSVSSEEAADLMNRARQPFNVNAPAMAAAVAALADQEFVQES
                     RRLNREGMGQMRDGIARLGLEQIPSVGNFLSFDVGGPCFPVYQALLEQGVIVRPIAEY
                     GLPNHLRVTVGLPEENARFLGALERVLGNS"
     gene            complement(2939..4036)
                     /gene="pheA"
                     /locus_tag="AC16PP_00660"
     CDS             complement(2939..4036)
                     /gene="pheA"
                     /locus_tag="AC16PP_00660"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR6480160.1"
                     /translation="MSTRDDRLKAVRDRIDAIDRELQKLLNERAACALEVGEIKQADP
                     DAEAPVFYRPEREAQILSRLKQENQGPLSDDDVARLFREIISCCLNLEQPLTIAYLGP
                     AGTYTEAATVKQFGHFAGTAALASIDEVFREVETGGAHYGVVPVENSTEGMVNHTLDC
                     FIDSPLKICAEVELPIHHAFMVAPDASGEGIEEILSHSQSLAQCRTWLDAHYPGVPRT
                     PVNSNAEAARLVAARTNAAAVAGDMAAERYGLRVIASHIEDQTDNKTRFLVIGQQRVG
                     PSGRDKTSILVSTRNEPGALYKILEPFHRHGISLSRIETRPARSGSWSYVFFIDFDGH
                     GSEPAIEAVMREVGDVAMEVRSLGSYPQAVV"
     gene            complement(4033..5121)
                     /gene="serC"
                     /locus_tag="AC16PP_00665"
     CDS             complement(4033..5121)
                     /gene="serC"
                     /locus_tag="AC16PP_00665"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIR6480161.1"
                     /translation="MNRAINFSAGPAALPTQVLQQAQAELLDYAGTGMSVMEMSHRSA
                     EFIDIAERAERDFRELLQISDDYHVLFLQGGATLQFAMVPLNLLGDRGTADYVNTGSW
                     STKAIKEAARLCSVNVVASAEEHGFDRIPDPAAWACSADAAYLHICSNETIGGVQFHS
                     FPDRAGGPPLVADMSSDILSRPIDVSRFGLIYAGAQKNVGPAGLTLVVVRKDLVGAAR
                     PGTPSMLDYAVHAEAGSMSNTPPTYAWYLAGLVFAWIRSLGGVAALDELNRRKAGKLY
                     AAIDGSNLFRAPVVVEDRSIMNVPFTLPDGELDAAFLAGAKDRGLLNLKGHRSVGGMR
                     ASLYNAVPESAVDALISYMQEFETDHFR"
     gene            complement(5118..7703)
                     /gene="gyrA"
                     /locus_tag="AC16PP_00670"
     CDS             complement(5118..7703)
                     /gene="gyrA"
                     /locus_tag="AC16PP_00670"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020299636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR6480162.1"
                     /translation="MSDLGSDQGREILPVNIEDELRQSYLDYAMSVIVGRALPDVRDG
                     LKPVHRRVLFTMSELNNTFNRPYMKSARVVGEVMGKYHPHGDVAIYDTVVRLAQDFSM
                     RYMLVDGQGNFGSIDGDPPAAMRYTEVRMARIAGELLADLDKETVSFVPNYDDTLTMP
                     EVLPTRVPNLLVNGSSGIAVGMATNIPPHNLREVVAACLALLDDPSLTVDDLMVHIHG
                     PDFPTAAIINGRAGIVQAYRTGRGRIYVRARAEVISDGKSNKDTILITEIPYQLNKAR
                     LIEKIAELVKEKKIEGITELRDESDKDGLRVVIELRRGEPGEVVLNNLYAQTQLQSVF
                     GINCVALVDGQPRVLNLKQMLGAFVQHRREVVTRRSIYLLRRARQRGHILEGQAVALA
                     NIDAVVELIKASRTGADARTALMSRGWMSEAVQKLLERAGSDACRPEGLGEEFGFREG
                     LYYLSEEQAQAILELRLQRLTALEQDKLIEDYQVILDEIADLQDILGSEERLVSVIRA
                     ELEEIRDTYGDERRTEILSSHEDLSAEDLITEEDLVVTISHQGYAKTQSLDTYQAQRR
                     GGRGKAATAVKDEDFVEHLLIANSHATLLCFSNLGKVYWLKAYQIPRASRGAKGRPLV
                     NMLPLAADERITVVLPIEDFAEDRFVFMATANGTVKKTPLEQFARQRSTGLIALELEE
                     GNTLVGAAFTDGHCDVLLVSSSGKAVRFKESDVRAMGRTARGVRGIRLAGDHRVISLI
                     IPAPDGLILSASENGYGKRTVVDEFPVKGRGGQGVIAMQRSDRNGDVVGVVQVFGGDE
                     LMLISNMGTLVRTAADEVSVQGRNTQGVRLINLRGDEKLVGVERIVDSESAEADEESA
                     DADEE"
     gene            7839..9158
                     /locus_tag="AC16PP_00675"
     CDS             7839..9158
                     /locus_tag="AC16PP_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HIR6480163.1"
                     /translation="MPSTPCDLIIDADWILPIVPAGSVLQEASIAIADGRILKIGDRE
                     LIHGEHQPREVHNLPGHALLPGLINAHGHAAMTLLRGFAEDAPLENWLSDHIWPTEAR
                     LVDADFVRDGARLAMAEMLKTGTTCFSDMYFFPEVTAAEARAAGMRAQIAFPVIEFPN
                     AWSASSDEGFHKGLALHDDYRRDPLIRVAFGPHATYSVSEGDLERILVYSEELDAHVQ
                     IHLHETAREVAEARDRVGMSWLRYLDERGLLGPNLQAVHATHLDDEEIDLLAENNVHV
                     VHCPLSNLKLASGICATTTLLARGVNVALGTDGAASNNGLDLLLEARLATLLAKLRDG
                     DAGALPAHRALEMATLGGARALGMEAQIGSLEPGKAADMVAIDLTGLKFQPLFDPVAQ
                     LVHTTSGDGVAHVWVAGRQLLRDGQLCTLDEQAILRAARGWRERISQ"
     gene            9155..9847
                     /gene="ubiG"
                     /locus_tag="AC16PP_00680"
     CDS             9155..9847
                     /gene="ubiG"
                     /locus_tag="AC16PP_00680"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HIR6480164.1"
                     /translation="MNVDQAEIGKFDALAARWWDPEGEFKPLHDINPVRLNYLMEHCD
                     IRSRPVVDVGCGGGILAESLAREGATVTGIDMAESPLAVARLHAEDSGVQVEYLATTA
                     EDLAAERAGRFGTVACLEMLEHVPDYASTVKACADLAAPDGKLVFSTINRNPKAYLLA
                     ILGAEYVLGLLPRGTHDYDKFIRPAELAAAVRAAGLTVKEIIGMRYNPFTRQCSLTPD
                     VDVNYMLYAEKS"
     gene            9831..10631
                     /locus_tag="AC16PP_00685"
     CDS             9831..10631
                     /locus_tag="AC16PP_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HIR6480165.1"
                     /translation="MPKSPEYPELEAARAGWRFQPEDDCLRGKVILVTGAGDGIGAAA
                     ARTYACYGADVVLLGRTRSKLETEFDWIEAETDTRPVIVPCDLRALDDASADALHDAI
                     ADGYGRLDGILHCASALGPKVPIALFPTADWQEVLQVNVTAPFLLTRTLLPLLNAAPA
                     ASVVLVSSTVGRKGRAYWGAYAVSKHALEGLMEVLADEHEHAGIIRVNSLNPGATRTA
                     MRAAAYPAENPASVPTPEARMDVFVYLMADASNGISGEALDAREWRGP"
     gene            complement(10703..11467)
                     /locus_tag="AC16PP_00690"
     CDS             complement(10703..11467)
                     /locus_tag="AC16PP_00690"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6480166.1"
                     /translation="MGRVEGKVAIVTGGASNPGLGRTTALTLAREGAIVVVTDVDLEG
                     AEATAAEIRDAGGRALALHQDVTDEGRWAEVIAATVDAHGRLDVLVNNAGIAVLMPLA
                     EMSLADWNRQIEVNLTSVFLGCKHAAPAMREAGGGSIVNLSSVAGLVGLPTCVAYGAA
                     KGGVRIMSKSVAVELAEHDIRCNSVHPGFIWTNMQAQSAGASAPGGGAMPRDRVPLGR
                     MGDPQDIANCVLYLASDESSYVTGAEFTVDAGMTAQ"
     gene            11657..12595
                     /locus_tag="AC16PP_00695"
     CDS             11657..12595
                     /locus_tag="AC16PP_00695"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR6480167.1"
                     /translation="MAEIIARDAKFADPTTTADGKPRASVAPTGLTTLWVNTGTLCNL
                     TCENCYIESSPTNDRLAYISRAEVRAYLDEIAGSDLETEEIGFTGGEPFMNPDIMGML
                     EDSLGAGHRVLVLTNAMRPMMKHARGLLALGSRYGDALTLRVSVDHFEPDRHRQERGP
                     RSWRPMMEGLRWLSESGFAVHIAGRTRWGEDEHTLRAGFAALFAAEEIAVDAHDPASL
                     VLFPEMDTSADVPEITTECWDLLGRRPEDQMCASSRMVVKRRGAETPEVVACTLLPYD
                     ERFAMGPTLASSLRPVALNHPHCAKFCVLGGGSCSA"
     gene            complement(12635..13048)
                     /locus_tag="AC16PP_00700"
     CDS             complement(12635..13048)
                     /locus_tag="AC16PP_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6480168.1"
                     /translation="MRHVGDILAEKSKDIVWIGPLESVYDALKLMNEKDVGAVLVQID
                     EYLVGILSERDYMRKVILDNHAAKQIKVSEVMTRDVVTVTREDTVVHCIALTKKHHVR
                     HLPVVEEGKAIGMISLRDLFLDVIHEKVGEVGDED"
     gene            13230..13388
                     /locus_tag="AC16PP_00705"
     CDS             13230..13388
                     /locus_tag="AC16PP_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480169.1"
                     /translation="MEDHNVGAKIFEDVFEFEDDFETEAEANVEAFYVALKMALARLE
                     AYATATPE"
     gene            complement(13391..>13728)
                     /locus_tag="AC16PP_00710"
     CDS             complement(13391..>13728)
                     /locus_tag="AC16PP_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015096290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase family protein"
                     /protein_id="HIR6480170.1"
                     /translation="DGERLQFSDIKYYDGRGTNHWYHVVLMEGRNREIRRLFESVGLS
                     VSRLKRVRFGPVALPSTLPRGRYAELSYEDLKTLYGLLKLPLRARPAKGGRGPRTSFL
                     LPYPDLPIV"
BASE COUNT         2377 a         4651 c         4387 g         2313 t
ORIGIN      
        1 gtcgtcgaca acctccatgg caacctgcac ctggtcgccc acggagaggg tgaactcacc
       61 ctcttcgttg atgaactggt tgcgggggat gaccccctcg gacttgagcc ccgcgtggac
      121 gatgacccag tcgttgtcta tatccaccac cgtgccggtg acgatggaac ccggttccat
      181 ctcgatggtc gtcaggcttt cttcaaacag atcggcaaaa ctgtcgctca tttaatttgc
      241 cgcgccggcc ggcgcgacgt ctccgtgttg tcgagcagca ccccagtgat accgcccggt
      301 tggttcattt caaatgcctt tcgccaggcc ccgtgtccgg gcctcggcca gcaccctgtc
      361 cagcacctga tcgatggtca gctcggtgct gtccacgagg atcgcgtcct gggcaggtcg
      421 cagcggcgat acggcacgcc tcatatcccg ctcatctctt tcacggatgc tcgcgagaag
      481 ggcgcggaag gtaacactta accctttgtt tttcaattga ttatagcggc gctccgccct
      541 gacctccggc gaggcctcca gatacacctt cagatccgcc tccgggaaca ccaccgtgcc
      601 catgtcccgg ccgtcggcaa ccagtccagg agcgcgccgc atggcgcgct gcaggccgaa
      661 aatcgcctgc cgaacgcctt cgagcgcggc cacctgcgac gccgccgagc tgacggcctc
      721 ggtgcggatg gtccgggtga gatcctcgcc atccaccagc acgcggtcgt cctcgaaacg
      781 gatgtcgagc cccgccgcca tcccggcaat ggccgcttcg tcgtccaggg cgagccctcg
      841 cgcaatggcc tgggccccga cgatgcgata cagcgccccg ctatccagca ggtgccaccc
      901 gagacgctcg gcaagcaggc cggccaacgt gccctttccg gagccgctcg gcccatccac
      961 cgtgatcacc ggcgggagac tcaacggcca tcgcctccaa tcatcaccgc ggccgcctca
     1021 gtcaccgtga tagccccgcc gccgctcgct cgcccgggcg atgcagtcgc gaaccgcgtc
     1081 ttcgtcttcg gcctcgatgg ctcgacgcag gtcggcgaga tccgcctcga accccgccag
     1141 cgcagcaagg accgcgtcct tgttcagccc gaagatcgcc gaccacatgt ccgggtccga
     1201 accgccgatg cggctgaagt cgcgaaagcc cccaccagcg tggtccagat gcgcaggctc
     1261 gatctgctgc atgtaggcga acgcgatgag atggggcagg tggctggtca gtgcgtacac
     1321 gcgatcgtgc agttcagggt ccatgctttc cacgcgagcg cccaggctgc gccaccagta
     1381 ggccacctgc tcccgcgcct ccgggaccgt gcctgccacc ggcgtctgca ccaccaggcg
     1441 ctccgcgaag aggtcctcgt tggccgcgcc cggtccgctg acctcccggc cggcaatggg
     1501 atggcagggc acgaagcgtt cgggaatctc gcccagctgc cccctgattg catccaggat
     1561 ggcccccttg acgctgccgg catcgaacag aatgccgggg tgatcggcca gccgcaccac
     1621 ccactcggcc accgcgtcgc tcggcgcggc gatcagcacc gcatcgctgg cggcagggac
     1681 ctcgtcggcg atctcgtcca cgatgccgag gctcaaggca gactgcgcgt tgcccgggtc
     1741 cggatcgacc ccgacgaagg tctggaaaag attacgggtg cgcgcgccga gggcgaacga
     1801 gccgccgatg agtccggtcc caacgacggt gagcagcatc ggcctaagaa ttcccgagaa
     1861 cgcgttccag cgcccccaga aagcgagcgt tctcctcggg cagcccgacg gtcacgcgca
     1921 aatggttggg aaggccgtac tcggcgatgg gccgtacgat gacgccctgc tcgagcagcg
     1981 cctggtagac gggaaaacag gggccgccca cgtcgaagct gaggaaattg cccacggacg
     2041 gaatctgctc gaggccaagc cgggcaatgc cgtcgcgcat ctgccccatc ccctcgcggt
     2101 tgagccttcg gctctcctgc acgaactcct gatccgcgag cgccgcaacg gcagcagcca
     2161 tggccggcgc gttgacgttg aagggttggc gggcccggtt catcaggtcg gccgcttcct
     2221 cggagctcac gctgaagccc actcgaaggg cggcaagccc gtgaatcttc gagaacgtgc
     2281 gcgtgacgat caggttcgga tagcgccgct gaagctccat gccgttaggg tatccggccg
     2341 cctccacgta ttcgtgatag gcctcgtcca gaacgaccca gacgtgctcg ggaacctcgt
     2401 ccaagaaccg caccaacgcc ccttcgccca cccaggtgcc ggtggggttg ttggggttgg
     2461 cgagaaagac gatggccgtg gcgtcggtga tggcatccag aattccgtcg agatccgcgc
     2521 cgtagttccg agccggcacc tcgacgagct cgccacccgc ggacagggtg gccaggcggt
     2581 agacgacgaa ggcgtgttcc gagatcaccg cttcggcccc cggggacagg ctcatgcgcg
     2641 cggccagatc gaggacgtcg ttggagccgt tgccaacggt gagctggctc tcccgcaccc
     2701 cgaaatgccg ggccagcgcc tgcttgagcg cgaacgcgtt gccgtccggg tagcgggcaa
     2761 ggtctccaca ggccgcatcg atggcctgcc gcaccagctc gccgggcccg cgagggttct
     2821 cgttgctcgc cagcttgatc acgtcgacca gcccgagttc ccgggtgact tcttctaccg
     2881 gcttgccggg ctgatacggc ttgagcgcct tgatcctggg gttaacgcgc ggcgacattc
     2941 agaccaccgc ctggggatag gagcccagcg aacggacctc catggccacg tcgccgacct
     3001 cccgcatgac ggcttcgatt gccggctcgc tgccatgccc gtcgaaatcg atgaagaaga
     3061 cgtaggacca gctgcccgat ctggccggcc gcgtctcgat acgcgacagg ctgatgccgt
     3121 gtcgatggaa gggctcgaga atcttgtaga gcgcgccggg ctcgttgcgg gtggaaacca
     3181 ggatggaggt cttgtcacga ccgctcggac ccacgcgctg ctggccgatg accagaaagc
     3241 gcgtcttgtt gtccgtctgg tcctcgatgt gggaggcgat gacccgcagt ccgtaacgtt
     3301 cggccgccat gtcgcccgca acggcggcag cattggtacg ggcggcaacc agcctcgctg
     3361 cctcggcatt gctgttgacg ggcgtgcggg gaacgccggg atagtgcgcg tccagccagg
     3421 tgcggcactg agcgagcgac tgggagtggg acagaatctc ctcgatgccc tcgccgctgg
     3481 cgtccggcgc caccatgaag gcgtgatgga tgggcagctc cacctccgcg cagattttca
     3541 gcggcgagtc gatgaagcag tcgagcgtgt ggttcaccat gccttccgtg gagttctcca
     3601 ccggcaccac gccgtaatgg gcgccgccag tttccacctc gcgaaacact tcgtcgatgg
     3661 atgcgagcgc cgccgttccc gcaaagtgcc cgaactgctt gacggtggcg gcttcggtgt
     3721 aggttcccgc cggcccgagg taagcgatgg tcagaggctg ctcgagattc agacagcagg
     3781 aaatgatctc gcggaaaagg cgcgcgacgt cgtcgtcgct cagcggcccc tggttctcct
     3841 gcttgagccg cgaaaggatc tgggcttccc gctccggccg atagaacacc ggcgcctccg
     3901 catccgggtc cgcctgcttg atctcaccca cctccagcgc gcaggcagcc cgctcgttca
     3961 ggagtttctg gagctcccgg tcgatggcgt cgatacggtc gcggaccgct ttgagcctgt
     4021 cgtcccgggt gctcatctga agtgatcggt ctcgaattcc tgcatgtacg agatcagcgc
     4081 atccaccgcg gattcgggca ccgcgttgta aaggctcgcc cgcatcccgc ccacgcttcg
     4141 gtgtcccttc agattgagca ggccgcggtc cttggcgccg gccaggaatg cagcatccag
     4201 ctcgccgtcg gggagcgtga acggcacgtt catgatggat cggtcttcaa ccaccaccgg
     4261 cgcgcggaac agattgctgc cgtcgatggc cgcgtagagc ttgcccgcct tgcggcggtt
     4321 gagctcatcg agggcggcga ccccgcccag ggacctgatc caggcgaaca ccagccccgc
     4381 caggtaccag gcgtaggtcg gcggggtgtt tgacatggac cccgcctcgg cgtgaacggc
     4441 gtagtccagc atgctgggcg tgccgggccg cgcggcccca accagatcct tgcgcacgac
     4501 gacgagcgtc agtcccgccg gcccgacgtt cttctgggcg ccggcgtaga tgagaccgaa
     4561 acggctgacg tcgatgggcc gggacaggat gtccgacgac atgtctgcca ccagcggcgg
     4621 gccgccggcg cggtcgggaa agctgtggaa ctgaacccct ccgatggtct cgttcgagca
     4681 tatatggagg taggcggcat cagcgctgca ggcccacgca gcaggatccg gaatccggtc
     4741 gaaaccgtgc tcctcggcac tcgcaaccac gttgaccgag cacaggcggg cggcttcctt
     4801 gatcgccttg gtcgaccagg agcccgtgtt gacgtaatcc gccgtgccac gatcgcccag
     4861 caggttcagg ggcaccatgg cgaactgcag cgtggcgcct ccctgcagaa acagcacgtg
     4921 atagtcgtcg ctgatctgca ggagttctct gaaatcccgc tcggcgcgct cggcaatgtc
     4981 gatgaactcc gcgctgcggt ggctcatctc catgaccgac atgcccgtgc cggcgtagtc
     5041 cagaagttca gcctgggcct gctgcagcac ctgtgtgggc agcgccgccg ggccggccga
     5101 gaagttgatc gcgcggttca ttcttcgtct gcgtcggcgc tttcttcgtc cgcctcagcg
     5161 ctctccgaat cgacgattcg ctccactccc acgagcttct cgtcgccgcg caggttgatc
     5221 aggcgaaccc cctgggtatt gcgcccctgc acggacacct cgtccgccgc agttcgcacc
     5281 agcgtgccca tgttgctgat gagcatcagc tcgtctccgc cgaagacctg gacgacaccc
     5341 accacgtcgc cgttgcggtc gctgcgctgc atggcgatga cgccctggcc gccacgtcct
     5401 ttcaccggaa actcgtccac caccgtgcgc ttgccgtagc cgttctcgct cgcgctcaag
     5461 atcaatccgt ccggggccgg aatgatgagg gagatcaccc gatgatcacc cgccaggcgg
     5521 atcccgcgaa cgcccctggc ggtgcggccc attgcgcgaa cgtccgattc cttgaagcgc
     5581 acggctttcc cggagctcga aacgagcagg acgtcgcagt gaccgtccgt gaacgcggcg
     5641 ccaacgaggg tgttaccttc ctccagctcc aacgcgatga gcccggtgga gcgctggcgg
     5701 gcaaactgct ccagcggcgt cttcttcacc gtcccgttgg cggtggccat gaaaacgaag
     5761 cgatcctccg cgaaatcctc gatgggaaga accaccgtga tccgctcatc cgccgccagc
     5821 ggcagcatgt tcaccagcgg ccggcccttg gcgccccggc tcgcgcgggg aatctgatag
     5881 gccttcagcc agtagacctt gcccagattc gaaaagcaca gcagcgtggc gtggctgttg
     5941 gcgatgagca ggtgctccac gaagtcctcg tccttcaccg ccgtggccgc cttgccgcgc
     6001 ccgccgcgcc gctgcgcctg ataagtgtcc agcgactggg ttttcgcgta gccctgatgg
     6061 gagatggtga ccaccaggtc ttcctcggtg atcagatcct cggccgagag gtcctcgtgg
     6121 gaggagagaa tctccgttct gcgctcgtcg ccgtaggtgt cccgaatctc ctcgagttcc
     6181 gctcgaatga ccgagacgag ccgctcttcg gagccgagaa tgtcctgaag atcggcaatc
     6241 tcgtcgagaa tcacctgata gtcttcgatg agcttgtcct gctcgagggc ggtgaggcgc
     6301 tgcagcctga gctccaggat cgcctgggcc tgctcctcgg agaggtagta gagcccctcg
     6361 cgaaagccga actcttcgcc caggccctcc ggccgacagg catcgctgcc ggctctctcc
     6421 agcagcttct ggaccgcctc cgacatccag cctctgctca tgagcgccgt acgcgcgtcc
     6481 gcacccgtcc gggaggcctt gatgagctcc accacggcgt cgatgttggc cagcgccacg
     6541 gcctggcctt ccaggatgtg gccgcgctgg cgggctcgac gcagcaggta gatcgaccgc
     6601 cgggtgacca cttcgcgccg gtgctgaacg aacgccccca acatctgctt taggttcagc
     6661 acccgaggct ggccgtctac cagggccacg cagttgatgc cgaacaccga ttggagctgg
     6721 gtctgggcgt agaggttgtt cagcaccacc tcgcccggct cgcctcggcg cagctcaatc
     6781 acgacgcgca ggccgtcctt gtcggactcg tcccgcagct ccgtgatccc ttcgatcttc
     6841 ttctccttga ccagctcggc gatcttctcg atgagccggg ccttgttgag ctggtagggg
     6901 atctcggtga tcaatatggt gtctttgttg gacttaccgt cactgatcac ttccgcgcgc
     6961 gcacgaacgt agatgcgccc gcgaccggtt cggtaagcct gcacgatgcc ggcccggccg
     7021 ttgatgatgg cggcagtggg aaagtccgga ccgtggatgt gcaccatgag gtcgtcgacg
     7081 gtcagcgacg gatcgtccag cagcgccagg caggcggcca cgacctcgcg aagattgtgg
     7141 ggcggtatgt tcgtggccat gcccacggcg ataccggagc tgccgttgac gagcaggttc
     7201 ggaacccggg tcgggagcac ctccggcatg gtgagggtgt cgtcgtaatt cggtacgaaa
     7261 ctcaccgttt ccttgtcgag gtcggccagc agctccccgg cgatgcgcgc catgcgcacc
     7321 tcggtgtacc tcatggctgc cggcgggtcc ccgtcgatgg agccgaagtt cccctggccg
     7381 tccacgagca tgtaacgcat ggagaagtcc tgggccagtc ggacgacggt gtcgtagatg
     7441 gcgacatcgc cgtgcgggtg gtacttgccc atgacctcac ctaccacccg ggcggacttc
     7501 atgtagggcc ggttgaaggt gttgttcagc tcgctcatcg tgaacagcac ccgcctgtgc
     7561 acgggcttga ggccgtcacg aacgtcggga agggcgcgcc cgacgatcac gctcatggcg
     7621 taatcgagat aagactgtcg aagctcgtct tcgatgttga ccgggaggat ctcccggccc
     7681 tgatcggacc ccaggtcgga catatggaac cgctgcagaa aagcgcgcca ttttaccacg
     7741 aagcgcaggc gaacctgata cttttgacca cggaattccg cccgtctccg cgctctatga
     7801 acccgcctac gcccggccgg aggggctata atcggccgat gccgtccacc ccatgcgatc
     7861 tgatcatcga tgccgactgg atactgccca tcgtgcctgc aggctcggtg ctccaggagg
     7921 cgagcattgc cattgccgac ggcaggatcc tgaagattgg cgatcgcgag ctgattcacg
     7981 gcgagcacca gccccgggag gtgcacaacc tgcccggcca cgcgctgctc ccggggctga
     8041 tcaatgccca tggccacgcc gccatgacgc tgctgcgcgg ctttgcggag gacgcgccgc
     8101 tggagaactg gctgagcgac cacatctggc ccaccgaggc tcggctcgtg gatgcggact
     8161 tcgtccgcga cggcgcccgg ctggccatgg ccgaaatgct caagaccggc acgacctgct
     8221 tctcggacat gtacttcttt cccgaggtga cggctgcgga ggcgcgggcg gccggcatgc
     8281 gggcccagat agcctttccc gtcatcgagt ttcccaacgc gtggagcgca agctccgacg
     8341 aggggttcca caagggcctg gcgctccacg acgactaccg gcgcgatcct ctcatccggg
     8401 tcgcgttcgg gccccacgcc acgtacagcg tctccgaggg cgacctcgag cggatcctcg
     8461 tgtactccga ggaactcgac gcccatgtgc agatccacct tcacgagacc gcgcgggagg
     8521 tcgccgaggc tcgcgaccgg gtcggaatga gctggctgcg ctatctcgac gagcgcgggc
     8581 tgctcgggcc gaatctgcag gccgtccacg ccacgcacct ggacgacgag gagatcgacc
     8641 tgctcgcaga gaacaacgtg cacgtggtcc attgcccgct gtcgaacctc aagctggcaa
     8701 gcggcatctg cgccaccacg accctgctcg cccggggggt gaacgtggcc ctgggaaccg
     8761 acggcgccgc gtcgaacaac ggcctcgacc tgctcttgga agcacgcctg gcaacgctgc
     8821 tggccaagct cagagacggc gacgcgggcg ccctgccggc ccatcgggca ctggagatgg
     8881 cgaccctcgg cggcgcgcga gccctgggca tggaagcgca gatcggttcg ctggaaccgg
     8941 gcaaggccgc ggacatggtc gccatcgacc tgaccgggct gaaattccag cccctgttcg
     9001 atccggtcgc gcaactggtc cacaccacgt ccggagacgg ggtggcccac gtctgggtgg
     9061 cggggcggca actgctgcgc gacggtcagc tgtgcacgct ggacgaacaa gccatcctgc
     9121 gggcggcgcg cgggtggcgg gagcgaatat cccaatgaac gtggatcagg cggagatcgg
     9181 caaattcgac gccctggccg cgcgctggtg ggatcccgaa ggggaattca agccgctcca
     9241 cgacatcaac cccgtgcggc tcaactatct catggagcac tgcgacatcc gctcgcgccc
     9301 ggtggtagac gtgggctgcg gcgggggcat tctggcggaa agcctcgctc gggagggcgc
     9361 tacggtgacg ggcatcgaca tggcggagag ccccctggcc gtggctcgcc tgcacgcgga
     9421 ggacagcggc gttcaagtgg agtacctggc taccaccgct gaggacctgg ccgcggagcg
     9481 cgccggcagg ttcggcacgg tggcctgcct ggaaatgctc gagcacgtcc ccgactacgc
     9541 ttccacggtg aaagcctgcg cagacctcgc agcacccgat ggaaaactgg ttttctccac
     9601 catcaaccgc aatccaaagg cgtatctgct ggccatcctg ggcgccgagt acgtcctggg
     9661 cctgctgccc agaggcaccc acgactacga caagttcatc cgccccgcgg agctcgcggc
     9721 ggccgttcgc gccgcagggc tgaccgtgaa agagatcatc ggcatgcgct acaacccgtt
     9781 cacacgacag tgctcgttga cgcccgacgt cgacgtgaac tacatgctct atgccgaaaa
     9841 gtcctgagta ccccgagctg gaggcggcgc gcgccggctg gcggttccag ccggaagacg
     9901 actgcctgcg aggcaaggtg atcctggtca ccggagccgg cgacgggatc ggcgcggcgg
     9961 cggcgagaac gtacgcctgc tacggtgccg acgtggtgct gctggggcgc acgcgatcca
    10021 agctggaaac cgagttcgac tggatcgagg ccgagacgga cacccggccg gtgatcgtcc
    10081 cctgcgatct cagagctttg gatgacgcaa gcgccgacgc cctgcacgac gccatcgccg
    10141 acggctacgg ccggctcgac ggcatcctgc actgcgcctc ggccctcggc ccgaaagtgc
    10201 ccatcgccct gtttccaacg gcggactggc aggaggtgct gcaggtcaac gtcaccgcac
    10261 ccttcctgct gacccgaacc ctgctgcccc tgctgaacgc ggcaccagcc gcgtcggtcg
    10321 tgctggtctc ctccacggtg gggcgcaagg gccgggccta ctggggagct tatgccgttt
    10381 ccaagcacgc gctggaaggc ctgatggagg ttctggccga cgagcacgag cacgccggca
    10441 tcatccgcgt gaacagcctg aacccgggcg ccacgcgcac ggccatgcgg gccgccgcct
    10501 atcctgccga gaaccccgcc agcgtgccga cgccagaggc gcggatggac gtgttcgtct
    10561 acctgatggc ggacgcctcg aacggcatca gcggggaggc gctggatgcg cgtgagtggc
    10621 gcggtccgtg acagccctgc tcgctgcggc gggccgatct caaaccaacg cggtaccgga
    10681 ggacctgaac ggagccggcc agctactgcg ccgtcatacc cgcatccacg gtgaactcgg
    10741 cgccggtgac gtagctcgac tcgtcggagg cgaggtagag aacgcagttg gcgatgtcct
    10801 ggggatcacc catgcgtccc agcggcaccc ggtcccgggg catcgcgcca ccgccggggg
    10861 ctgacgcacc cgcggactga gcctgcatgt tggtccagat gaatccgggg tggaccgagt
    10921 tgcagcgaat gtcgtgctcg gcgagctcca cggcgacgct cttggacatg atccggaccc
    10981 cgcccttggc cgccccgtaa gcgacgcagg tgggcaggcc cacaagcccg gccacggacg
    11041 agaggttgac gatggagccg ccgcctgcct cccgcattgc cggtgccgcg tgcttgcagc
    11101 caaggaagac gctggtcagg ttgacctcga tctgccggtt ccagtccgcc agagacatct
    11161 cggcaagggg catgagcacc gcgatcccgg cgttgttgac caggacgtcc agcctgccgt
    11221 gagcatccac cgtagcggcg atgacctcgg cccagcggcc ctcgtcggtg acgtcctggt
    11281 gcagcgccag cgcccttcct cccgcatccc ttatctccgc cgcggtggcc tcggcacctt
    11341 ccagatccac gtcggttacc acgacgatcg cgccctcgcg cgccagcgtc agcgccgtcg
    11401 tgcggccgag gccgggattg gacgccccgc ccgtcacgat ggccactttg ccctcaactc
    11461 gtcccatatc gccgctcccg gtgtgtcgac tgcagaaaag aggtcgcgag tatggcaaac
    11521 cggcttgtgt cggacaaagc tcgtctttcc ggacgaaccc gtgtacgccc gttgcggggg
    11581 agaaggcggc ctgtcagcat gagagaccga tcgagcagga cgagcgccgc aaacgccggc
    11641 gcaagggcaa tcagagatgg ccgagatcat cgcgcgggac gcgaagttcg ccgacccgac
    11701 caccacggca gacggcaagc ccagggcatc cgtggctcca accggactga cgaccctgtg
    11761 ggtcaatacc ggcaccctgt gcaacctgac ctgcgagaac tgctacatcg agtccagccc
    11821 gaccaacgac cgcctggcct acatctctcg cgccgaggtt cgagcatatc tcgacgagat
    11881 cgccggctcc gacctcgaaa ccgaggagat cggctttacc ggcggcgagc cattcatgaa
    11941 ccccgacatc atgggcatgc tcgaggacag cctaggagcg ggccatcgcg ttctggtgct
    12001 caccaacgcc atgcggccga tgatgaagca cgcgcgcggg ctgctggccc tgggctcccg
    12061 ctacggggac gctctgacgc tgcgggtttc agtcgaccac ttcgagcctg accgccaccg
    12121 acaggagaga ggcccgcgtt cctggcggcc gatgatggag gggttacgct ggctgagcga
    12181 gtccggcttc gccgttcaca tcgccgggcg cacccgctgg ggagaggacg agcacaccct
    12241 tcgggccggc ttcgccgccc tgtttgccgc cgaggagatc gccgtcgacg cgcatgatcc
    12301 cgcgagcctg gtgctgttcc cggagatgga caccagcgcc gatgtgcccg agatcaccac
    12361 cgaatgctgg gatctgctgg gacggcggcc ggaggatcag atgtgcgcaa gctctcgaat
    12421 ggtggtcaag cgcagggggg cggaaacccc cgaggtggtc gcgtgcacgc tgctgcccta
    12481 cgacgagcgc ttcgccatgg gccctacgct ggcttccagc ctgcggccgg tcgcgctgaa
    12541 ccatccccac tgcgccaagt tctgcgtcct cggcggcggc agctgctcag cctgacgctc
    12601 ctcacacgat ggacggccgc tggacgcgcc gctgtcagtc ttcgtcgccg acttcaccca
    12661 ccttctcgtg gatgacgtct agaaagaggt cgcgcagcga gatcatgccg atggcctttc
    12721 cttcctccac cacgggaaga tgacggacgt ggtgcttttt cgtcagcgcg atgcaatgga
    12781 cgacggtgtc ttcccgggta accgtgacca cgtcccgggt catcacctcg gagactttga
    12841 tctgcttggc cgcatgattg tccagaatca ctttccgcat gtagtcccgc tctgacagga
    12901 tcccgacgag atactcatcg atctgcacga gcacagcgcc cacgtccttt tcgttcatga
    12961 gcttgagcgc gtcgtacacg gattcgagcg ggccgatcca gacgatgtct ttggacttct
    13021 cggccagtat gtcccctacg tgccgcattc agtatccccc cagattgccg ccgcctaaca
    13081 gtagatcgcc ccgacgctgt aagcaaccgc ccgatgaccg ggtcagcggg tacctgccat
    13141 ggcgcgaaac tgggaaccgc gtcacagccc ggggggacga cttgaataag atctggctca
    13201 ccaaccaaca acaacgggat agtcagatca tggaagatca caacgtcggc gcaaagatct
    13261 tcgaagacgt ctttgagttt gaggacgatt tcgaaaccga agcagaggca aacgtggaag
    13321 cgttctacgt cgccctcaag atggcactcg cccggctcga agcgtacgcg accgccacgc
    13381 cggaatagcc tcagacgatc ggcaggtcgg ggtacggcag cagaaaagag gtgcgcggac
    13441 cacgcccgcc cttggcgggg cgagcccgca gcggcagctt gagcaggccg tacagggttt
    13501 tcagatcctc gtaactcaac tcggcatagc gtccgcgagg gagggtagaa ggaagcgcca
    13561 cgggaccgaa ccggacccgc ttgagccggc tgaccgacag gccgacactc tcgaacagtc
    13621 gcctgatctc acgattccgg ccctccatca gaaccacgtg ataccagtga ttggtgccac
    13681 gcccgtcgta gtacttgatg tccgagaatt gcagccgctc gccgtcga
//
LOCUS       DBNHKV010000026         6441 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1491912, whole genome shotgun sequence.
ACCESSION   DBNHKV010000026 DBNHKV010000000
VERSION     DBNHKV010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6441)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1491912"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="AC16PP_00715"
     CDS             <1..755
                     /locus_tag="AC16PP_00715"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011061221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6480171.1"
                     /translation="HVAIADVHADGLDETRRRVQAVAGVRCSTHLVDVADRAAVEAFA
                     ADTVREHGGVDLIFNNAGVDVTATVEEMSYADFEWLVNINFWGVVHGTKAFLPYLRQA
                     GQGHVVNTSSLFGLMSVPTQSAYHAAKFAVKGFTDSLRLELADTDIGVSCVMPGGVRT
                     NIVRNARFRISDNQAPTQDEVVERFESRAGLSSEQAAEWILRGVSRNKARILVGRDAQ
                     IAAFLLRVFPVSYLKFMTWMRRRNERVDRRND"
     gene            772..1557
                     /locus_tag="AC16PP_00720"
     CDS             772..1557
                     /locus_tag="AC16PP_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6480172.1"
                     /translation="MSDEVVLYEESENVGVVTLNRPEAMNSLTYQSYLELEDRVRECS
                     ARVLVITGQGRAFCAGDDVKHILGSSQPAPPEFAERARRSGGLTPAADALLHTNIPVI
                     AAVNGPAVGWGMELALMADIRVASENAKFGELFVIRGLCCDAPGLGRLAQLVGRERAA
                     ELLFTGEVIDAETARSIGLVSRVVPQEDLMATALDIARRIASNPPLAVQALKAGLRRT
                     LEPDWRETGRWASEQIAALRKTEDSKEGVAAFLEKRPPVYVGR"
     gene            1597..2490
                     /locus_tag="AC16PP_00725"
     CDS             1597..2490
                     /locus_tag="AC16PP_00725"
                     /inference="COORDINATES: protein motif:HMM:NF003784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HIR6480173.1"
                     /translation="MSNFDDQLLKIESANGFIAALDQSGGSTPKALGLYGVDSSAWSN
                     DEEMFEIVHQMRARIMTSSSFTGERILAAILFENTMDREVEGRPTADYLWDVKNVVPI
                     LKVDKGLADEAQGVQVMKPMPDLDDLLTRAKAKGIFGTKMRSVIKQADAGGIKTVVDQ
                     QFEIGRRIVAAGFVPIIEPEVDIHCPDKAGAEQILKGALLAGLDALRPEERVMFKLTL
                     PEADNFYAECIDHPNLVRMVALSGGYSREEANARLARNNRMVASFSRALTEGLSAQQT
                     QAEFDAALDASIQSIFEASLT"
     gene            2540..3622
                     /gene="hppD"
                     /locus_tag="AC16PP_00730"
     CDS             2540..3622
                     /gene="hppD"
                     /locus_tag="AC16PP_00730"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HIR6480174.1"
                     /translation="MTNRVDNPMGLDGFEFVEFSATQPGQIEPVFAMLGFQEVARHRS
                     KDVRLWRQGGINLVVNYEPRSVAWYYAEEHGPSACGLAFRVRDAHVAYKRALELGAQP
                     VEIPTGPMELRLPALRGIGGAPLYLIDRYRPGSSIYDIDFEYLDAVDRFPEGIGLEII
                     DHLTHNVYRGRMDHWARFYETLFNFQEIRHFDIRGEQTGLSSRAMTAPDGKIRIPLNE
                     EARGAGDGQVEEFLLQYNGEGIQHIAFACQDLPSKLDRLAAAGVPLMEPPPDAYYDML
                     EDRLPGHGEPVGELKQRGILLDGSTGEGEPRLLLQIFSEPLIGPIFFEFIERKRDEGF
                     GEGNFQALFESMERDQIRRGAIEGPS"
     gene            3619..4489
                     /locus_tag="AC16PP_00735"
                     /pseudo
     CDS             3619..4489
                     /locus_tag="AC16PP_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003200383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
     gene            4592..5110
                     /locus_tag="AC16PP_00740"
     CDS             4592..5110
                     /locus_tag="AC16PP_00740"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015094380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6480175.1"
                     /translation="MVAEAKPVGPKRFRESYGRYLEDFAVGDVYEHRPGRTITETDNT
                     WFTLLTMNQHPLHFDKEYGANSEFGRVLVNSCLTLSLVTGMSVSDVSQKTVANLGWNN
                     VRLSGPVFVGDTIYAESEVLEIRESKSRPNQGIVTIRSRGIKQDGTEVISFERAMLIP
                     ARGHAVDDAIDY"
     gene            5121..6269
                     /locus_tag="AC16PP_00745"
     CDS             5121..6269
                     /locus_tag="AC16PP_00745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6480176.1"
                     /translation="MAELSEQDRLVLDSVDRFLDRDVRPVARALEQEDAYPHELVEKM
                     KALGLFGATIDERHGGLGLTASVYALIVERVSAVWMSVSGIFNSHLIMCAAVQRFGTE
                     AMKERLLPAMATGELRGGIALTEPDCGTDLQAIRCRARRDGESYVIDGTKQWITNAVE
                     GNVLAVLVKTDPDAVPAHRGMSLLIVEKSAGYKATKLKKLGYRGVDTGEVQFDGVRVP
                     AANLVGDGEGRGLQQILSGLALGRINVAARGVGVADASLQAALRYAQERQTFGVPIHD
                     HQAIQIKLADMATRVEAARLLTEKAARIYDTGQRCDLEAGMAKLFASEAALSNSLEAM
                     RIHGAYGYSPDNDVERYYRDAPLMAIGEGTNELQRIIIARQLIERNPI"
     gene            complement(6295..6429)
                     /locus_tag="AC16PP_00750"
     CDS             complement(6295..6429)
                     /locus_tag="AC16PP_00750"
                     /inference="COORDINATES: protein motif:HMM:NF016230.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR6480177.1"
                     /translation="MDAGVQTFTQLEESCGAGTGCGRCREVAQELIDQRLADSAYYAA
                     "
BASE COUNT         1201 a         1912 c         2170 g         1158 t
ORIGIN      
        1 gccacgtcgc catcgccgac gtgcacgccg acgggcttga cgagacgcgc aggcgggtgc
       61 aggccgttgc cggcgtgcga tgctccaccc acctcgtcga tgttgccgat cgtgctgcgg
      121 tggaggcgtt cgcagcggat acggtgcgcg agcacggcgg cgttgacttg atattcaaca
      181 acgcgggggt agacgtgacc gccaccgtgg aggaaatgag ctacgcagac ttcgagtggc
      241 tcgtgaacat caatttctgg ggggtcgtgc acggcaccaa ggcgttcctt ccctacctcc
      301 ggcaggctgg gcaagggcac gtggtcaaca cgtcgagcct gttcgggctc atgtccgtgc
      361 cgacgcagtc ggcctaccat gccgccaagt tcgccgtgaa gggctttacc gactctctga
      421 ggctcgagct ggctgacacg gacatcggcg tgagctgcgt gatgcccgga ggggtccgga
      481 cgaatatcgt gcgcaatgcg aggttccgga tatctgacaa ccaggcgccc acccaggacg
      541 aagtggtcga gcggttcgag agccgggcgg ggctgagctc ggagcaggcc gcagagtgga
      601 tactgcgagg tgtttcgcgc aacaaggctc gaatcctcgt ggggcgtgat gctcagatcg
      661 cggcgttcct gctcagggtg tttccggtaa gctatctgaa attcatgacg tggatgcgac
      721 gccgaaacga gcgggtcgac cgtcgcaacg actgaacgag gggctgagaa catgagtgac
      781 gaggtagtgc tgtacgagga gagcgaaaac gtcggcgtcg tcacgctcaa tcgcccggaa
      841 gccatgaatt ctcttaccta ccagtcttat ctcgagctcg aggaccgggt gcgcgagtgc
      901 tcggcccggg tgctcgtcat caccgggcag gggcgcgcgt tctgtgccgg tgacgacgtc
      961 aagcacatcc tcggcagcag ccagccggcg ccgcccgagt tcgcggagcg cgcccgccgc
     1021 agtggtggtc tgacccccgc ggcggacgcg ttgctccaca ccaacattcc cgtcatcgcc
     1081 gcggtcaacg ggccggccgt gggctggggc atggaactcg cgttgatggc tgacattcgg
     1141 gtggcctcgg agaacgccaa gttcggtgag ctgttcgtaa ttcgagggtt gtgctgcgat
     1201 gcccccggtc tcggccgtct cgcccagctc gttggccgcg aaagggccgc ggagctgctg
     1261 ttcacaggcg aggtcatcga cgccgagacc gcgcgaagca tcgggctggt ctcccgggta
     1321 gtgccccagg aagatctcat ggcgaccgcc ctggacatcg cccgcagaat agcctccaat
     1381 ccgccgctgg ccgtgcaggc cttgaaggcc gggttgcgcc gaaccctcga gcccgactgg
     1441 cgggaaaccg gtcgttgggc aagcgagcag atcgccgcgc tcagaaagac tgaggactcg
     1501 aaggagggcg tcgcggcatt cctcgaaaag cgcccgcccg tctacgtggg tcgttgaccc
     1561 gttccggagt aaccaaccga taagtgagca tgacaagtga gtaatttcga cgaccaactc
     1621 ctgaagatcg agtcagcaaa cggattcatc gccgcgctgg accagagtgg cggcagcacg
     1681 cccaaggccc tgggtctgta tggggtcgat tcaagcgcgt ggtccaacga cgaggagatg
     1741 ttcgagatcg tgcatcagat gcgcgcgcgc atcatgacga gctcgagctt caccggcgag
     1801 cggatcctcg cggccatcct tttcgagaac acgatggatc gcgaggtgga gggccgcccc
     1861 acggccgact acctctggga cgtgaagaac gtggtaccca tcctcaaagt cgacaagggg
     1921 ctggcggacg aggcgcaggg cgttcaggtg atgaagccca tgccggacct ggacgatctg
     1981 cttaccagag caaaagcaaa gggcatcttc ggcacgaaga tgcgctcggt gatcaagcag
     2041 gcggacgcag gagggatcaa gaccgtcgtg gatcagcagt tcgagatcgg tcgccgaatc
     2101 gtcgccgcgg gattcgtgcc catcatcgag cccgaggtgg acattcactg tccggacaag
     2161 gctggagccg agcagattct caaaggcgct ctcttggccg gcctggatgc cctgaggccc
     2221 gaggagaggg taatgttcaa gctcaccttg cccgaggcgg acaactttta cgcggagtgc
     2281 atcgatcatc cgaatctggt gcggatggtg gcgctctccg gcggctactc acgggaggag
     2341 gccaatgcgc ggctcgctcg caacaaccgg atggtcgcca gcttctcgcg ggcgctgacg
     2401 gagggacttt ccgcgcagca gacccaggca gaattcgacg cggcgctcga tgcctccatt
     2461 caaagcatct tcgaggcctc tctaacctga agcgccagcg cggccagctg cctgaacccg
     2521 tcacggcgat gcgataagga tgacgaaccg cgttgacaat cccatgggcc tcgacggttt
     2581 cgagttcgtc gagttttccg caacgcagcc gggtcagatc gagccggtct tcgcgatgct
     2641 cgggtttcaa gaagtggccc ggcatcgctc caaggacgtc cgcctctggc ggcagggcgg
     2701 gatcaacctc gtcgtcaact acgagccgcg aagcgttgcc tggtactacg ccgaggagca
     2761 cgggccttcg gcatgcggtc tcgcattcag ggtccgcgat gcccacgttg cctacaagcg
     2821 ggcgctggag ctcggcgccc agcccgtcga gattcccaca ggcccgatgg agctgcgcct
     2881 gccggccctc cgcggaatcg gcggcgcacc gctgtacctg atcgatcgct atcggccggg
     2941 cagcagcatc tacgatatcg acttcgaata tctcgacgca gtggatcggt ttcccgaggg
     3001 tatcggtctc gaaatcatcg atcaccttac gcataacgtc taccgggggc ggatggatca
     3061 ttgggcccgt ttctacgaaa cgcttttcaa ctttcaggag atccgacatt tcgacattcg
     3121 gggtgagcag acgggcttga gctcccgggc catgaccgcc ccggacggca agatccggat
     3181 accgctcaat gaggaagccc gtggagccgg cgacgggcag gtggaagagt ttcttctgca
     3241 gtacaacggt gagggaatcc agcacatcgc ttttgcctgc caggacctgc cgtcgaagct
     3301 ggatcgtctg gcagcggccg gggtgccatt gatggagcct ccgccggacg cctactacga
     3361 catgctggaa gatcgccttc cgggtcacgg ggagcccgtg ggcgaactca agcagcgtgg
     3421 aatcctgttg gatgggagta cgggggaggg tgagccgagg ctgctgctgc agatcttctc
     3481 cgagccgttg atcggcccga tatttttcga gttcatcgag cgcaaacgcg acgaagggtt
     3541 cggagaaggc aacttccagg cgctgttcga atccatggaa cgggatcaga ttcgtcgcgg
     3601 ggccatcgaa ggaccatcgt gaagcttgca acgcgagggg aggatcggga tggtgagctc
     3661 ctcgtcgtct cgcgggatct gagccgggcg gttcgcgtgg gggaaatcgc tcccacgttg
     3721 cagggcgccc tcgaccgatg gggtacggtt gctgaaaagc ttgcctccgt ctacgaacga
     3781 ctcaacgacg gtgccctgga agcctcgttt ccattcgtcc ccgcacgttg cgcggcgccg
     3841 ctgccgcgcg cttaccaatg ggcggacggc agtgcctacg tcaatcacgt ggagctggtc
     3901 cgtcgcgcgc ggggtgccga catgcccgcg agcttctggc gtgaacctct gatttatcag
     3961 ggcggatccg acgtcatgct ggggcccacc gatgacatcg tcgtgaggag cagcgagtgg
     4021 gggctcgatt tcgaggccga gatcgccgtc atcgtcgatg acgtcgagat gggcacctcg
     4081 acggtcgatg ccggtgacca cataaagctt ctcatgctcg tcaatgacgt ctcgctgcgc
     4141 gggctgatac ccggggagct ggacaaaggc ttcggcttct atcaatccaa gccggccacg
     4201 gcgttttcgc cggtggccgt gtctcccgac gagctttgcg gcgactggcg tgagaacaag
     4261 gtgcaacgcc ccctgttgag ctatgtcaac gggcggctct tcggtcgcgg ccggcggcgt
     4321 gggctactcg tgcattgcag agctgcgcat gatcgagacc attcgcgatg gggcgccgca
     4381 gacgtccttc ctgcaggcgg gggatcgggt caccatcgaa atgctcgacc gggacggctc
     4441 cagcatcttc ggctccatcg accagcgggt ggtggtcggg accggctgaa ttgtcattcg
     4501 gggtcgtgaa tgtgttctac ttttgtcccg aagcgaggcc gggcaaacgc gcgcttcggg
     4561 agcgggcgac gcaaccgagg aggtggcaaa catggtggca gaggcaaagc cggtggggcc
     4621 taagcggttc cgagagtcct acgggcgata tctggaagat ttcgccgtgg gcgacgtgta
     4681 cgaacatcga ccgggacgga cgatcactga aactgacaac acctggttca cgctgttgac
     4741 catgaaccag catccgcttc atttcgacaa ggagtacggg gcgaattccg agttcgggag
     4801 ggtgctggtc aactcctgcc tgacgctgtc tctggtgact ggtatgtcgg tctctgacgt
     4861 cagccagaag acggtggcca atctgggctg gaacaacgtc aggctgagcg ggccggtgtt
     4921 cgtcggcgac acgatttacg ccgagtcaga ggttttggag atccgggaat ccaaatcccg
     4981 cccgaatcag ggcatcgtga cgatccgttc tcgcggcatc aagcaggacg gcacggaggt
     5041 catcagcttc gagcgcgcca tgctcattcc ggcccggggc cacgccgtgg acgatgccat
     5101 cgactattga ggcttgtgtc atggccgaac tctcagaaca ggaccggctc gtgctggact
     5161 ccgtcgatcg gtttctcgat cgggacgtac gaccagtggc gcgcgcgctg gagcaggaag
     5221 acgcctatcc tcacgagcta gtggagaaga tgaaggcgct cggcctgttc ggcgccacca
     5281 tcgacgagcg tcatgggggg ctgggactga ccgcatccgt gtacgcgctc atcgtcgagc
     5341 gcgtgtccgc cgtctggatg tctgtgtcgg gcatcttcaa ctctcatctg atcatgtgtg
     5401 ccgccgtgca acgtttcggc accgaggcga tgaaggaacg ccttctcccg gcaatggcca
     5461 cgggcgagct gcgagggggg atcgccctca ccgagccgga ctgtggaacc gacctgcagg
     5521 ccattcgttg ccgtgcccgc cgcgacgggg agagctacgt gatcgacggg acgaagcagt
     5581 ggatcaccaa cgcggtggaa ggcaacgtcc tggccgtgct ggtgaaaacg gatccggatg
     5641 ccgttccggc tcacagaggc atgagcctgc tcatcgtcga aaaatccgcg ggttacaaag
     5701 cgaccaagct caagaagctc ggctatcgcg gcgtggacac cggcgaggtg cagtttgatg
     5761 gggtgcgggt gcccgcggcc aacctggtcg gcgatggtga gggccggggt ctgcagcaga
     5821 tcctctcggg actggcgttg ggccggatca acgttgcggc ccggggggtg ggggtggccg
     5881 atgcttcgct tcaggctgcc cttcgctacg cccaggagcg ccagacgttt ggcgtgccga
     5941 tccacgatca tcaggccatt cagatcaagc tggccgacat ggccacccgt gtggaggctg
     6001 cccggctgct cacggagaag gctgcccgaa tttacgatac gggacagcgc tgcgatctcg
     6061 aggcaggcat ggcgaagctc ttcgcatcgg aggccgcgct cagcaactcc ctggaagcga
     6121 tgcgcatcca tggtgcttac gggtactcgc ccgacaacga tgtcgagcgc tactatcgag
     6181 acgcgccgct catggcgata ggcgagggaa ccaacgagct gcagagaatc atcatcgccc
     6241 gccagctcat cgagcggaat cccatctgag aatgcggtcg ggccggcgga tcagtcaggc
     6301 agcgtagtac gccgagtcgg caaggcgctg atcgatgagt tcctgtgcca cctcgcggca
     6361 gcggccgcag ccggtgccgg ccccgcagga ttcctcgagc tgagtgaagg tctgaacgcc
     6421 ggcgtccacg gcttcgtgga t
//
LOCUS       DBNHKV010000027         3841 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_287971, whole genome shotgun sequence.
ACCESSION   DBNHKV010000027 DBNHKV010000000
VERSION     DBNHKV010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_287971"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="AC16PP_00755"
     CDS             complement(<1..553)
                     /locus_tag="AC16PP_00755"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HIR6480178.1"
                     /translation="MLATLSRQLDSERAMVRRGRGLDWLRIWPFIGLHLACLAVFWVG
                     VSPVAVVVAVACYLIRMFAITAFYHRYFAHKAFRTSRACQFVFAVLGAASTQRGPLWW
                     AAHHRRHHMHTDTADDPHRPQDGFLWSHMGWFLSGEHFQTDMARVRDWSVFPELVWLN
                     RFDTVVPILLGTAMFALGWGLERL"
     gene            complement(654..2261)
                     /locus_tag="AC16PP_00760"
     CDS             complement(654..2261)
                     /locus_tag="AC16PP_00760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6480179.1"
                     /translation="MAKWQQPRSFWTWGYRSDEPSEEERRQAAARVSERLGRVVEPPP
                     IPRIGEIALRKPRLSVPAGLRGWVTTDHEERVVHTHGGHPLELLSAVRGEFDAPPDAV
                     AHPRNETELEATLAWCDEHGHAAIPFGGATSVVWGVRVPESADAAVTIDMDHFHQVLE
                     IDEVSRAARIQAGVLGPDLENTLRPLGLTLRHFPQSFPWSTVGGWVATRSGGHYATNH
                     THIDDFVESVRMLTPAGWWESRRLPGSGAGPSPDRMVMGSEGILGVISECWLRLQKRP
                     VYRATAGVVFDSWEAGAEAARQIVQAKLWPANLRLLDPAEAAQSAGLGGGRSLLIVGF
                     ESADLPQGDLMRDALAIARGAGGLVDDDAVRVDDGSGKATGREGSVGAWRSAFIGVNA
                     GLTSGLGLLADTFETAITWDRWPEFDASVRERVGRVLEEVLGPGARLSCRFTHVYPDG
                     PAPYYSFSGVAGLGAEHEQWRVIKDEATSAVVDAGGTVTHHHAVGRMHRAGWEDQHPP
                     LYAQALRAMKRTLDPNGILNPGVLIDP"
     gene            complement(2254..3528)
                     /locus_tag="AC16PP_00765"
     CDS             complement(2254..3528)
                     /locus_tag="AC16PP_00765"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR6480180.1"
                     /translation="MAGERTLPVRLALAVALLVSSASAEPAAAGESTVIVLSWDGMRH
                     DYPDRGDFPGLARVISEGARAERLVTVFPSSTFPGHVSLATGTFPDRHGIVGNHFVDR
                     QRGVYRMDSDADWLLAEPIWIAAERQGVMAATYFWVGSESDWRGRGTRYRESPFDGSR
                     PEALKVDRILEWLQLPEGQRPRLIMSYWAGADRIGHRFGPDSERVTTQIAAQDLQLQR
                     LLQGLDLLDRWASTTLLIVSDHGMTASYRYTDLRGMLAAAGIGAYVTGSTVVHVYLDD
                     PDDHARAGELLGKIEGARAYPGDALPGWMRLSHAARTGDWLVVATPPLTFSRPAGWEG
                     AMVATLSMVGWTFGSHGYPPDHPEMPGMFLALGRGVPAGALLRAVHQVDLAATIAALL
                     GIEPPLESEGTPVPGLEIPVAARQAAKEDDDG"
     gene            complement(3518..>3841)
                     /locus_tag="AC16PP_00770"
     CDS             complement(3518..>3841)
                     /locus_tag="AC16PP_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013934.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR6480181.1"
                     /translation="TGCAAATSSGKRAGKGAGYSDLEFAILRELGHDPVPVATTVHDV
                     QLVEDFPIAHNDLPLSLICTPTRTIRVQAPPAAPAGIQWGELSAEQLAAMPVLESLRR
                     LKHGG"
BASE COUNT          665 a         1339 c         1291 g          546 t
ORIGIN      
        1 ccagacgttc gagcccccat cccagagcga acatggccgt gccgagcagg atgggcacca
       61 ccgtgtcgaa ccggttgagc cagaccagct ccgggaagac gctccagtcg cgaacccggg
      121 ccatgtcggt ctgaaaatgc tcgccggaga gaaaccagcc catgtgggac cacaggaagc
      181 cgtcctgtgg gcggtgcgga tcgtccgcgg tgtccgtgtg catgtgatgt cggcggtggt
      241 gcgccgccca ccacagcggt ccccgctggg tggatgccgc gccgagcacg gcaaacacga
      301 actggcaggc gcgcgaggtg cgaaacgcct tgtgcgcaaa gtaacgatgg tagaaggccg
      361 tgatcgcaaa catccggatc aggtagcagg ccacggcgac cacgaccgcc accgggctca
      421 ccccgaccca gaacacggcc aggcaggcca ggtgaaggcc gataaagggc cagatgcgca
      481 gccagtccaa gccgcggccg cggcgcacca tggcccgttc ggaatccaat tgcctggaaa
      541 gcgtggccag catcgccccc accctgaacg aagaggacgg ccactatgcg cggccgcgcg
      601 tgaaagccac gtcaacaggg gagcagacga gcgagtcgcg ggcgtgggcc gtcctaaggg
      661 tcgatgagca cgccgggatt gaggatcccg ttgggatcca gggtgcgctt catcgctctg
      721 agcgcctggg catagagagg tggatgctga tcctcccacc ccgcacggtg catgcgccct
      781 acggcgtggt gatgcgtcac cgttccgccg gcgtccacca ccgcagacgt ggcctcgtcc
      841 ttgatgaccc gccattgctc gtgctctgcc ccgagcccgg caacgccgga aaagctgtaa
      901 tagggagcgg ggccgtcggg gtagacgtgg gtgaagcggc aactgagcct cgctcccggc
      961 ccgagcacct cctcgagcac ccgacccacc ctttcgcgaa ccgacgcgtc gaactcgggc
     1021 cagcgatccc aggtgatggc cgtctcgaag gtgtccgcga gcagaccgag cccgctggtc
     1081 agcccggcgt tgacgccgat gaaggccgat cgccaggccc ccacggatcc ttccctgccg
     1141 gtggctttcc ccgacccgtc gtccacgcgg accgcatcgt cgtccaccag gccgcccgct
     1201 ccccgtgcga tggccaacgc gtcgcgcatc agatcgccct gaggcaaatc cgcggactcg
     1261 aaaccgacga tgagcaggga ccggccgccg ccgaggcccg cgctctgggc agcctcagcc
     1321 ggatccagca ggcgcaggtt ggcgggccag agcttggcct gcacgatctg cctggcggct
     1381 tccgcacccg cctcccagct gtcgaacacc acgcccgccg tggcccgata aacgggccgt
     1441 ttctgcagcc gcagccagca ttccgagatg acgcctagaa tgccttccga gcccatgacc
     1501 atgcgatcgg ggctgggtcc ggcgccgctg ccggggagac gccgagattc ccaccatccg
     1561 gccggggtga gcatgcgaac gctctcgacg aagtcgtcga tgtgggtatg gttggtcgcg
     1621 tagtgcccgc cggaacgggt cgcgacccag ccaccaacgg tggaccaggg aaagctctgg
     1681 gggaaatgcc tgagcgtcag gcccagagga cggagggtgt tctccagatc gggccccagg
     1741 acgcccgcct ggatgcgggc cgcccgggac acttcgtcta tctccagcac ctgatggaag
     1801 tgatccatgt cgatggtcac cgcggcatcc gcgctctcgg gcacccgcac gccccagacc
     1861 actgaggtgg cgcccccaaa gggaatcgcg gcgtggccgt gctcgtcgca ccaggcgagc
     1921 gtcgcctcga gttctgtttc gttgcgcgga tgggctaccg catccggcgg cgcgtcgaac
     1981 tctcctcgca cggcagagag cagctcgagc gggtgaccgc cgtgggtgtg caccacacgc
     2041 tcctcatgat cggtggtcac ccaaccccgc aggccggcag gaacggacag ccgcggcttg
     2101 cgcagcgcaa tttccccgat ccgcgggatg gggggcggct cgaccacccg gccaagccgt
     2161 tcggataccc gagccgcggc ctgacggcgc tcctcctccg agggctcatc agaccgatag
     2221 ccccacgtcc agaagcttcg gggttgctgc cacttagcca tcgtcatcct ccttggccgc
     2281 ctgccgggcg gcaacgggaa tctccaggcc cggcaccggc gtgccctcgg attcgagcgg
     2341 gggctcgatg cccaggagcg cggcgatggt ggcagccaga tccacctgat gcaccgcccg
     2401 tagaagtgcc cctgccggaa cgccacggcc gagggccagg aacatgcccg gcatctcggg
     2461 atggtccggc gggtagccgt ggctgccgaa agtccatccc accatgctca gggtcgccac
     2521 catggctccc tcccaaccag ccggccggct gaaggtcagg ggaggagtag cgaccaccag
     2581 ccagtcgccc gttctggccg cgtgggacag gcgcatccaa cccggcagcg catccccagg
     2641 gtaggcccgc gcgccctcta tcttgccgag cagttcccct gcccgggcat ggtcatccgg
     2701 atcgtcgagg tagacgtgca ccaccgtgct gcctgtgacg taggcgccga tgccggcggc
     2761 ggccagcatg ccgcgcagat cggtgtaacg gtagctcgcg gtcatgccgt ggtcgctaac
     2821 gatcaggagc gtggtagacg cccagcgatc cagcaggtcg aggccctgga gcagccgctg
     2881 cagctgcaga tcctgagcgg ctatctgggt ggtgacgcgc tcgctgtcgg gaccgaaacg
     2941 gtggccgatc cgatcggcgc cagcccagta gctcatgatg aggcgcggcc gctgcccctc
     3001 cggaagctgc agccactcga gaatccgatc caccttcaaa gcttcgggtc gggaaccgtc
     3061 gaaggggctc tcgcggtaac gggtgccccg gccgcgccag tcgctctccg agcccaccca
     3121 gaagtaggtc gccgccatga ccccctggcg ctcagccgcg atccagatgg gctcagccag
     3181 cagccagtct gcatcggagt ccatcctgta gacgccgcgc tgccggtcga cgaagtgatt
     3241 gccgacgatg ccgtgccggt ccggaaacgt gcccgtggct aaagagacgt gtcccggaaa
     3301 agtagaggaa ggaaacacgg taacgagccg ctcggcccgg gcaccttcgc tgatcacccg
     3361 ggccaggccg ggaaagtcgc ccctgtccgg atagtcgtgg cgcatgccgt cccaggaaag
     3421 cacgatcacc gtgctctccc cggcggcggc gggctcggcc gaagccgagc tgacaagaag
     3481 cgccacggcc agtgccaggc gcacgggaag ggtgcgctca cccgccatgc ttgagcctgc
     3541 gcaaggattc cagaaccggc atggccgcca gctgctcggc ggagagctcg ccccattgaa
     3601 tcccggcagg cgcggcgggc ggcgcctgga cgcggatcgt gcgcgtcggc gtgcagatca
     3661 ggcttagggg caggtcgttg tgggcaatgg gaaagtcctc taccagctgc acgtcgtgga
     3721 ccgtcgtggc caccgggacg gggtcgtggc cgagctcccg caggatggcg aactccaggt
     3781 cgctgtaccc ggcgcctttc cccgcccgct tgcccgacga ggtagccgcc gcgcacccgg
     3841 t
//
LOCUS       DBNHKV010000028         3041 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1421279, whole genome shotgun sequence.
ACCESSION   DBNHKV010000028 DBNHKV010000000
VERSION     DBNHKV010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1421279"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="AC16PP_00775"
     CDS             <1..198
                     /locus_tag="AC16PP_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6480182.1"
                     /translation="AGAVAERLDVEMINVALAYVLNQPFPCFPLIGPRLPWETRSSLA
                     ALEVALSDAERAWLDLSADGG"
     gene            complement(179..640)
                     /locus_tag="AC16PP_00780"
     CDS             complement(179..640)
                     /locus_tag="AC16PP_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480183.1"
                     /translation="MILIRSGQWTGRGSILAEGQSLGQGVACDVEVQRDDEGASISAE
                     VDAKELGSWAMNVRIAPDEQGTYDLSVRTLKGSLTGTAKLDSPPNLGLLWNEEQTVHA
                     TFTLFEIAGGLGFRGFLRDGKTTYTWEIALSLRQDHIGGENVVSLVSRRRR"
     gene            complement(637..1335)
                     /locus_tag="AC16PP_00785"
     CDS             complement(637..1335)
                     /locus_tag="AC16PP_00785"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HIR6480184.1"
                     /translation="MCGRFNVTSSPLSELLLELVGLPHPGPDNYNAAPTETIVVLRMN
                     DDGRPELVAMRWWLTPFWSKSVTTKYSMFNAKSETVEKSPAFREPFSRRRCVVPVSGF
                     YEWARSKERKLPYYLTPHLASGMLLAGIWDSWQDGENGPLLSFAVLTCEANEQLTFVH
                     KRQPVMLGMKDARIWLDPSTQASALKALCAPRVPVALDAVPVSSYVNNARNKDSRCTE
                     AIGTAVAIREDVTP"
     gene            complement(1347..1994)
                     /locus_tag="AC16PP_00790"
     CDS             complement(1347..1994)
                     /locus_tag="AC16PP_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6480185.1"
                     /translation="MAHVQPRSIDDVPALKPIFDAAESAMGFVPNSMLTMAHMPQLPM
                     GFMMLTNVIFGGDLKAMADQLQPLIPEQGDAGENLSPPLIQLIAFATSISSGCRYCQA
                     HTAHSGHRQGAEQEKFDSVLDYENSPVFSDAERAAIGLALAAGAVPNQADAMHFERLR
                     AYFSERQIVQIVAVIAIFGFLNRWNDTMATQLEAPAVDYAAASLGELAWAIGKHG"
     gene            2093..>3041
                     /locus_tag="AC16PP_00795"
     CDS             2093..>3041
                     /locus_tag="AC16PP_00795"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF014896.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR6480186.1"
                     /translation="MIRPPVAKRRNHRHTHHGITLEDAYYWLRDPAYPDVKDPEIIEY
                     LTAENAYFDARMEAHQPLVTQLFEEIKGRQPAEDASVPHRHGAFWYQWRYQKDAQYRQ
                     WLRAPAPAGSSPDAGPWEVILDEPELAREHPYFSLGGISVSRSGRFIAWSADTSGAER
                     FTLRIRDLQTGELVDDPIEQTQGSPVWAADDTCFFYVVVNDQWRPYQVRRHKLGDPPD
                     ADAVVYEEPEDSFFVSVDETQSEAYILIAAGDHVTSEIRYVPADEPLAEPALIARRRA
                     GHEYEVDHREGAFYVVTNDAHKNFRLARAPEDDPVEQNWQ"
BASE COUNT          564 a          996 c          940 g          541 t
ORIGIN      
        1 gctggcgccg tcgccgagcg gctggacgtt gagatgatca acgttgcgct ggcctatgtc
       61 ctgaaccagc cgtttccctg cttcccgctc atcggcccga gattgccgtg ggagacccgc
      121 agcagcctcg cagcgcttga agtggcgctg agcgacgcgg agagggcctg gctggatctc
      181 agcgccgacg gcggctgaca agcgaaacca cgttctcccc gccgatatgg tcctgccgca
      241 gcgagagggc gatctcccag gtgtaagtgg tcttgccgtc gcggagaaac ccccggaagc
      301 cgagcccgcc ggcaatctcg aaaagagtaa acgtcgcgtg aacggtctgc tcctcgttcc
      361 agaggagccc caggttcggg gggctgtcca gcttggccgt tccggtcaga gaacccttga
      421 gggtgcgcac gctcaggtca tacgtcccct gttcgtccgg ggcgatcctg acgttcatgg
      481 cccacgagcc cagctccttg gcatccactt cggcgctgat gcttgcaccc tcgtcgtccc
      541 gctgcacctc cacatcacag gcaaccccct gccccagact ctgcccctcg gcgaggattg
      601 agccccggcc cgtccactgg cccgatcgaa tgagtatcat ggcgtgacgt cctccctgat
      661 ggccacggcc gtgccgatgg cttcggtgca gcgggaatct ttgttgcggg cattgttgac
      721 gtagctggac accggcaccg cgtccagcgc gacgggaacg cgcggagcac agagcgcttt
      781 gagggcggag gcctgcgtcg aaggatcgag ccagatccgg gcgtctttca taccgagcat
      841 aacgggctgg cgtttatgaa cgaacgtcag ctgctcgttc gcctcgcagg tgagcaccgc
      901 gaagctcaac agcggtccgt tttcaccgtc ctgccagctg tcccatattc cggccagcag
      961 catgccggaa gccagatgag gcgtcaggta atacggcagc ttgcgctcct tgctgcgagc
     1021 ccactcgtag aacccgctga ccggcaccac gcatcgacgc cggctgaagg gttcccggaa
     1081 agccgggctt ttctccacgg tctccgactt ggcgttgaac atgctgtact tcgtcgtcac
     1141 cgacttggac cagaaggggg tgagccacca gcgcatcgca acgagctccg gccgcccgtc
     1201 gtcgttcata cgcagaacga cgatggtctc cgtcggcgcg gcattgtaat tgtctggtcc
     1261 cggatgcggc agcccgacca gctcgagcag cagctccgaa agcgggctgg aggtaacgtt
     1321 gaaacgtccg cacacggcgc gcgccctcag ccgtgcttgc cgatggccca ggcgagctcg
     1381 cccagtgacg ccgccgcgta gtccaccgcg ggcgcctcga gctgggtcgc catggtgtcg
     1441 ttccagcggt tgaggaaccc gaagatggca atcaccgcca cgatctgcac gatctgccgc
     1501 tcgctgaaat acgcccgcag cctctcgaag tgcatagcat cggcctgatt gggcacggca
     1561 ccggcagcaa gcgccagacc gatcgccgcg cgctcggcgt ccgagaagac ggggctgttc
     1621 tcgtagtcga gaacggaatc gaacttttcc tgctcggcgc cctgacggtg tccgctgtgg
     1681 gcggtgtggg cctgacaata acggcagccc gagctgatgc tggttgcgaa ggcaatgagc
     1741 tgaatcagag gcggcgacag gttctcgccg gcatccccct gctcggggat cagcggctgg
     1801 agctgatcgg ccatggcctt gagatcaccc ccgaagatca cgttcgtcaa catcatgaaa
     1861 cccatcggca gctgcggcat gtgggccatg gtcagcatgc tgttgggcac gaaccccatg
     1921 gcgctctcgg cagcatcgaa tatcggcttc agcgccggga cgtcgtctat cgatctgggc
     1981 tgcacgtgcg ccatgggctt gcgctccaat gagttgggtt gcttgagttt actggtttgc
     2041 gttttggctt acattcgcca cactgccgag ccaacgaacg ctcgaggctc gaatgatccg
     2101 accacccgtc gccaaacgcc gcaaccaccg ccacacccat cacggcatca ccttggagga
     2161 tgcgtattac tggctgaggg atcccgccta ccccgacgtc aaggatcccg agatcatcga
     2221 atatctgacg gcagagaacg cgtacttcga tgctcggatg gaggcccacc agcccctggt
     2281 gacgcaactg ttcgaggaga tcaagggtcg tcagcccgcg gaggacgcct ccgtgcccca
     2341 tcgacacggc gccttctggt atcagtggcg ctatcagaag gatgcccagt accgacagtg
     2401 gctgcgcgca ccggcacccg cgggttcctc tcctgatgcc ggaccatggg aagtgattct
     2461 cgacgagccg gagctcgccc gggaacaccc gtacttcagc ctgggcggaa tctccgtgag
     2521 ccgcagcggc cgcttcatcg cgtggagcgc cgacacgagc ggagccgaac gcttcactct
     2581 caggatcagg gatctgcaaa caggcgagct cgtcgacgat cccatagagc aaacccaggg
     2641 aagcccggta tgggcggccg acgacacgtg ctttttctac gtggtcgtca acgatcaatg
     2701 gcgcccgtat caggtgcgcc gccacaagct cggcgacccg cccgatgctg acgccgtcgt
     2761 gtacgaggag cccgaagact cgttcttcgt ttccgtggac gagacccagt ccgaagccta
     2821 tatcctcatt gccgccggag atcacgtgac ctcggaaatc aggtacgttc cggctgacga
     2881 gcctcttgca gagccggctc tgatcgcgcg gagaagggcg ggccacgagt acgaagtgga
     2941 tcatcgcgaa ggcgccttct acgtcgtcac caacgacgcg cacaagaact tccgcctcgc
     3001 ccgagccccg gaagacgatc ctgtcgagca gaactggcag a
//
LOCUS       DBNHKV010000029        10396 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_854689, whole genome shotgun sequence.
ACCESSION   DBNHKV010000029 DBNHKV010000000
VERSION     DBNHKV010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10396)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10396)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10396
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_854689"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1200)
                     /locus_tag="AC16PP_00800"
     CDS             complement(100..1200)
                     /locus_tag="AC16PP_00800"
                     /inference="COORDINATES: protein motif:HMM:NF025627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HIR6480187.1"
                     /translation="MSRSHLLRTGLIHHDRESAFAGYTLFCGGQPASAHLIDMQGRIC
                     RRWENDMGISYAQLLADGNLLCRASSLPEVGGVRGLNGQSPCVYELDWDGNLVWAFHD
                     DWLHHDQHRLANGNTLLLAWRRVPAEITARVAGGRRSADDPEEMLGDVILEVDPSGAI
                     VRQWNSWEHLDPEEDAICPIDHRLEWTHANSITTTPSGEWLISLRRTNTVMAIEPDSG
                     EIAWRWGPGTLAHQHDARILDNGNLLVFDNGAHRSGEAEYSRVLEVDVPSDEIVWRYQ
                     SNPPFHFYSFMAGGAQRLPNGNTFICDAAVGRLFEVSPVGEIVWEYVNPFYASNPRLG
                     GDINIVFKASRHGPDDPGLAAKHLDPNRYENA"
     gene            1318..1959
                     /locus_tag="AC16PP_00805"
     CDS             1318..1959
                     /locus_tag="AC16PP_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_1061 family FMN-dependent PPOX-type
                     flavoprotein"
                     /protein_id="HIR6480188.1"
                     /translation="MGDETFAAFEGVVSDEESLRQVLGPPMQLAVDKVIDHIDDVSRA
                     IIEKSPFALLASATADAYPDVSPKGDPAGFVRVIDENYIAIPDRPGNRRGDTLTNLLD
                     NPYLAVIFLIPGKGETLRVSGECRIVRDRALRESMAVKGRVPELAIVMHVERVFIHCP
                     KCVVRSSLWDPQAWPDTSDLPNVAEAMIAHTKVKITPDELVEIAEKEDVVRLY"
     gene            2047..3303
                     /locus_tag="AC16PP_00810"
     CDS             2047..3303
                     /locus_tag="AC16PP_00810"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACS family MFS transporter"
                     /protein_id="HIR6480189.1"
                     /translation="MSALPRRHVVVGLAALSVFVCYIDRVNISVAIIPMTEDLGWSLA
                     TQGTVLSSFFVGYLLLQIVGGRLADRFGGKVVLGVGVLVWSLFTVLTPPAAYLGLGVL
                     IAARIGMGMGEAVTFPSIYSLYGRWVPLSERARAVGFTNSGIPLGTVFALVATPYIVE
                     WLGWEWAFYLFGAVGVFWFVAWHRFVTAAPQAHPGVCDDELQVIEAGTQVAQAGESPP
                     LRVFLKLPAVWAIVVAHFCNNWSLYVLLSWLPTFVNKGLGVDYSAVGWFTMIPHLTAF
                     VFLNVAGGVADRLIHRGMDVGRVRKLMQTIGFGGIAAALLVVGEVQSAWMAIGVMSVG
                     NALGAFCTGGFAVNHMDIAPRHAGTLMGITNTAGTLPGIVGVFVSGVILELTGSWALV
                     FQVAAGVNLFGLVFYLTFASGRRLID"
     gene            complement(3367..3879)
                     /locus_tag="AC16PP_00815"
     CDS             complement(3367..3879)
                     /locus_tag="AC16PP_00815"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR6480190.1"
                     /translation="MFRKVKGGTRMAPAMGERDEIRGELTHFSPADAANEATIGATIV
                     IKGEITGDENLVIEGTLDGTANLSDRNLTIAQTGRILGNVSANSITVEGEIQGNLTAT
                     ERVFVAKTGKVLGDIMAPRVSVEGGAKFTGRIDMDTKGGRVVRPSGPKRSETLTRPAA
                     ETAPAAENKA"
     gene            complement(4010..4495)
                     /locus_tag="AC16PP_00820"
     CDS             complement(4010..4495)
                     /locus_tag="AC16PP_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR6480191.1"
                     /translation="MTEHDMERYQDPHVIQGALNQRRIAVVGLSGNPLRASNFVGYYM
                     KRHGYEVVPVNPREEEILGLKSYPDLAAVPEPVGVVDVFRDPSAVPDIARAAVAAGAK
                     YLWLQFGVISFEGAEIAEAGGMQVIMDRCIKIEHARYRGRMHWLGFNTNQISAQRRRA
                     D"
     gene            complement(4497..5807)
                     /locus_tag="AC16PP_00825"
     CDS             complement(4497..5807)
                     /locus_tag="AC16PP_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012614627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR6480192.1"
                     /translation="MSESTDRPATDDTHQFGFHTRSVHAGSRPDPATGARALPIYQTS
                     SYVFEDSESAAAYFNLQEYGNTYSRIMNPTVAALEERIANLEGGAGAVAFASGLAAQS
                     AAFFTLLEPGDHVVASAALYGGTVTQLKHLFRKMSVALTFVDPDRIDTWEAALQPDTK
                     LLFAETIGNPGGNVLDIEPLAELAHRHGMPLMVDNTFATPYLTRPMEWGADIVVHSAT
                     KFLGGHGTSIGGLVVDSGEFDWSNGRFPVIAEPSPAYHGLAFHETFGTYGYLMKLRAE
                     TLRDLGAAMSPFNAFLFAQGLETLPLRMRQHVANAEAVAAYLDGREDVERVSYPGLPG
                     SPYRDRVARYLPRGAGAIFCFDLPGGREAGQRFIEQLTLFSHLANVGDAKSLVIHPAT
                     TTHRQLSDEELAAAGIGPGTIRVSIGIEDADDIIWDLEQGLAGI"
     gene            5949..7124
                     /locus_tag="AC16PP_00830"
     CDS             5949..7124
                     /locus_tag="AC16PP_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6480193.1"
                     /translation="MSAFRDDVRGWLEENCPPSMRTPLPADEYPGGGRRAVYGNPDTG
                     VWMQRMAERGFTVPLWPTEYGGAGLDAGENQVLQEELRRINARPALLGMGISMIGPAL
                     LEFGTEAQKREHLPKIARGEIWWCQGYSEPNAGSDLAGLGTSAVEDGDDYIVNGQKIW
                     TSGADRADWIFCLVRTDPSAPKHEGITFILFDMASPGVTVKPIELISGYSPFCETFFD
                     NVRASRRNVVGEPNKGWTVAKRLLQYERTSIGGIGGSSQKAATLEEIAREALGTVDGR
                     IADADLRAGILRHRMNDRAFGLTMKRSVQQTAAGIAPGAESSFFKYYGTEQNKGRYEL
                     LMSLLGTRGLGWSGPGFSDEERDATRAWLRSKANSIEGGTSEVQLNVIAKRILGLPD"
     gene            7126..8334
                     /locus_tag="AC16PP_00835"
     CDS             7126..8334
                     /locus_tag="AC16PP_00835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6480194.1"
                     /translation="MALGDRHYRWVVVGYTLVIQGVSIGMLVYSFALFAIPWLDLFAA
                     PRRDVMLTVAILQLVNGAVSPLAGRAMDRYSIRTIVLAGLALMLVGLVVVSRAQALWQ
                     VWLVYATLFPVSMAMMSTLASQTLVTRWFDSRRGVAIGISATGTNVGGIVFPLLIAGW
                     LTDFGWRETFLWLAVTAAVVVGPLSWFVLGRTPAGHHPATRTTPGGGSVWTTRAILST
                     RRFWIPGLAILPLNLAFGAVQYNLGVLSRDLGLATGTAANLIALHAVCMIAGKFFFGS
                     LGDRIDHRYLFWLSAGLMVAALLVLQGETPVWMLVTGIVMMGLAGGGILPMFGMVFGA
                     RFGVAAFGRVMGLVMTTMTIGAMGPLLAGWAYDLTGSYDAAFLGMALLLLPAAVAMIW
                     LPQPGTDTAS"
     gene            8434..9210
                     /locus_tag="AC16PP_00840"
     CDS             8434..9210
                     /locus_tag="AC16PP_00840"
                     /inference="COORDINATES: protein motif:HMM:NF018777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HIR6480195.1"
                     /translation="MIRITFLLRRKPELSAEEFYRYWREEHGPLVASHASRLNTLRYV
                     QVHTIDDPANQAMATARGGMEAPYDGVAEMWWESRAALVASLETKGGRRAGAELVEDE
                     ARFIDLPNSPMWLGHEYPQVNPTPENLVARERDTLVKLYFPLRPVQGLDERDAQSYWY
                     SNHGPIIRRQAAGVGIQRYVQVHRVADELEAGLRKARGTQVEGYMGHAELWFDRTVLG
                     VSTPERRAANERAIADESNFIDFRRSCMWLAKEHVFVDRR"
     gene            complement(9227..>10396)
                     /locus_tag="AC16PP_00845"
     CDS             complement(9227..>10396)
                     /locus_tag="AC16PP_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007942990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6480196.1"
                     /translation="EALMTIMAPLKQEVKKQGLWACHLTPALGGGGWGQLQLALMNEI
                     LGRSGWAPSLFGCQAPDSGNAEILAHYGTPEQKDRYLEPLLNNEICSCYSMTEPQGGA
                     DPKVFTCRAELIGDEWVINGEKWFSSNARYASFLIVMAVTDPEAPPYQRMSMFIVPTE
                     TAGIEIVRHVAVGYESKGAHAYIRYNDVRIPRDHMLGEPGQGFIVSQVRLGGGRIHHA
                     MRTMGKARRAFDMMCERALSRETQGEVLAQKQLVQAMIADSWIDIEQFRLFVLRTAWK
                     IDKLKNYKEVRADISAVKAQMPKVYHDVYARALQVHGSLGLSTEMPFAAGVLDSFHMG
                     LADGPTEVHKVTVAKQVLGQYQGTDDLFPEYHLIRRKEAALAKYADIIEHEVGNA"
BASE COUNT         1544 a         3396 c         3562 g         1894 t
ORIGIN      
        1 agtctcggca acgtccgcgc gcgaggcggg cccgctgacc gcgcaggcct gaagggctcg
       61 cggctccggc gcgccggacg aacgtgcatc ccgcgccttt caggcgttct cgtaccgatt
      121 ggggtccagg tgctttgctg cgaggcccgg gtcgtcgggc ccgtggcggc tcgccttgaa
      181 gacgatgttg atgtcgccgc cgagcctggg attgctggca tagaacgggt tgacgtactc
      241 ccagacgatt tcgccgacgg gcgagacctc gaagagcctg cccactgccg catcgcagat
      301 gaacgtgttt ccgttgggta accgctgggc gccgccggcc atgaagctgt agaagtgaaa
      361 gggcgggttg gactggtagc gccagacgat ctcgtcgctc ggcacgtcca cctcgagcac
      421 tctcgagtac tcggcctccc ccgaccggtg cgcgccgttg tcgaacacga gcaggttgcc
      481 gttatcgaga atccgggcgt cgtgctggtg ggccagggtg cccgggcccc agcgccaggc
      541 gatctcgccg gaatctggct cgatggccat caccgtgttc gtgcggcgca ggctgatcag
      601 ccactccccg gacggggtgg tggtgatgga gttggcgtgg gtccattcca ggcgatggtc
      661 gatggggcag atcgcgtcct cctcggggtc gaggtgctcc cacgagttcc actgcctgac
      721 gatcgcgccg ctcgggtcga cctcgaggat cacgtccccg agcatctcct cggggtcgtc
      781 cgcgctgcgc cggccgccgg cgacccgggc ggtgatctcc gctggcacgc ggcgccaggc
      841 gagcagcagg gtgttgccgt tggccagacg gtgctggtcg tggtgcagcc agtcgtcgtg
      901 aaaggcccag accaggttgc cgtcccagtc gagctcgtag acgcacgggc tctggccgtt
      961 cagcccgcga acgccgccca cctcgggcag cgaggaagcc cggcacagca ggttgccatc
     1021 ggccagcagc tgggcataag agatgcccat gtcgttttcc cagcggcggc agatcctgcc
     1081 ctgcatgtcg atcagatgcg cgctcgcagg ctgcccgccg cagaacaggg tgtaaccggc
     1141 aaacgccgat tcccggtcgt ggtgaatcag gccggtgcga agcaggtggg atcggctcat
     1201 ggtcgtgatg gtatcggctt cggggcgcga gcgtcacgtc gggaggggcg ccttgattcc
     1261 gttgcggggt atggaccatg atcgggagac gatcgcgcca cgagatagga ggggatcatg
     1321 ggcgacgaga cgtttgcagc gttcgagggc gtcgtctccg acgaggagtc acttcggcag
     1381 gtgctcggcc cgcccatgca gctggcggtg gacaaggtca tcgatcacat cgacgacgtc
     1441 agccgggcaa tcatcgagaa atccccgttt gccctgctgg catcggcgac cgccgacgcg
     1501 tatccggacg tctctcccaa gggcgatccg gccgggttcg tgcgcgtcat cgacgagaat
     1561 tacatcgcca tcccggaccg gcccggtaac cgccgcggcg acacgctcac caacctgctc
     1621 gacaacccct atctggcggt gatctttctg atcccgggca agggcgagac gttgcgggtc
     1681 agcggcgaat gccggatcgt gcgcgatcgg gcgctccggg aaagcatggc cgtgaaaggt
     1741 cgtgtgcccg aactcgccat cgtcatgcat gtggagaggg ttttcattca ctgtcccaaa
     1801 tgcgtggtgc ggtccagttt gtgggaccct caggcctggc cggacacctc ggatctgccc
     1861 aacgtcgccg aggccatgat tgcccacacc aaagtgaaga tcactcccga cgagctcgtc
     1921 gagatcgcgg agaaagagga cgtggtcagg ctctactgag cccgtggcga ttccctcccc
     1981 ggacgtcgtg acggccccgc gcctggttga cgcggcgcgg ccccgtccgg gaccgagcag
     2041 cgcacgatga gcgcgcttcc ccgtcggcac gtggtggtgg gcctggctgc tctgtcggtg
     2101 ttcgtctgct acatcgaccg ggtcaacatc tccgtggcga tcatccccat gaccgaggat
     2161 ctcggctgga gcctggccac tcagggcact gtgctgtcgt ccttcttcgt cggatacctg
     2221 ctgctgcaga tcgtcggtgg ccggctcgcc gaccgcttcg gcggcaaggt ggtgcttggc
     2281 gttggcgtcc tcgtctggtc gctgttcacg gtactgacac cgcccgccgc ctacctcggc
     2341 ctcggggtgc tgatcgcggc ccgcatcggt atgggaatgg gcgaagcggt gacgtttccc
     2401 tcgatctaca gcctctacgg ccgctgggtg ccgttgagcg agcgcgcccg agctgtcggg
     2461 ttcaccaaca gcggcatccc gctgggcacc gtttttgcgc ttgttgcgac gccgtacatc
     2521 gtcgagtggc tcggctggga gtgggccttt tacctctttg gcgcggtggg cgtgttctgg
     2581 ttcgtggcct ggcaccgctt cgtgaccgca gcgccccagg cgcaccctgg cgtctgcgat
     2641 gacgagctgc aggtcatcga ggcgggtact caggttgcgc aggcgggcga gtcgccgccg
     2701 cttcgggtct ttctcaagct gcccgccgtc tgggccatcg tggtcgccca cttctgcaac
     2761 aactggtcac tctacgtgct gctgagctgg ttgccgacgt tcgtcaacaa gggcctgggc
     2821 gtggactact ccgcggtggg atggttcacg atgattccgc acctgacggc cttcgtcttc
     2881 ctcaacgtgg ccggcggcgt tgccgaccgg ctcatccatc gcggaatgga cgttggccgc
     2941 gtccgcaagc tgatgcagac catcggcttc ggcggcatag cggccgcgct gctcgtcgtc
     3001 ggcgaggtgc agtccgcatg gatggccatc ggcgtcatgt ccgtcggcaa cgcgctgggc
     3061 gcgttctgca ccggcggctt tgccgtcaac cacatggata tcgcgccgcg ccatgcgggt
     3121 acgctcatgg ggatcaccaa cacggcggga acgttgccgg gcatcgtggg cgtgtttgtc
     3181 agcggcgtga tcctcgagct gaccggatcc tgggccctgg tgtttcaggt ggccgccggg
     3241 gtcaacctgt tcggcctcgt gttctatctc acgttcgcca gcggcaggcg cctgatcgat
     3301 tgatcaggcg cgcgagcgcg ggacgacctc gtcgcgaggt gccccgcatc cgcggctgcg
     3361 gcgttctcag gccttgtttt cggcggccgg ggcggtctcg gcggccgggc gggtcagggt
     3421 ctccgagcgc ttcggacccg acggcctgac gacccggcca cccttggtgt ccatgtcgat
     3481 ccggccggtg aacttggcgc caccctcgac gctcaccctg ggcgccatga tgtcgccgag
     3541 gaccttaccg gtcttggcga cgaacacccg ctcggtggcg gtcaggttgc cctggatttc
     3601 cccctcgacg gtaatcgagt tggcgctgac gttgccgaga atccgacccg tctgggcgat
     3661 ggtgagattg cggtccgaga ggttggcggt accgtccagg gtcccctcga tgaccaggtt
     3721 ctcgtcgccc gtgatctctc ctttgatcac gatcgttgct ccgatcgtgg cctcgtttgc
     3781 tgcgtctgca gggctgaagt gcgttagctc tccgcgaatc tcgtcccgtt cccccatggc
     3841 gggtgccatg cgggtgcccc ctttgacctt tctaaacatg ccttcggcct cgtgtcccaa
     3901 ttccccatgc cccctttctt ttgttcgatt ggggctcctt tcaccgtgca gattaactga
     3961 gcaccggccg aattgacaag tagctatatc caggtcggcg ctgcggattt cagtccgccc
     4021 ggcggcgctg tgcggagatc tggttggtat tgaagccgag ccagtgcatg cgcccccgat
     4081 agcgtgcgtg ctcgatcttg atgcagcggt ccatgatcac ctgcatgccg ccagcttcgg
     4141 cgatctcggc gccctcgaag ctgatcacgc cgaactgcag ccacaggtac ttcgcgccgg
     4201 ccgccaccgc ggcgcgcgca atgtccggca ccgcgctcgg gtcgcggaag acgtccacca
     4261 cgcccacggg ctccggcacg gctgcaagat ctggatagct tttcagcccg aggatctctt
     4321 cctcccgggg attgacggga accacctcgt agccgtgccg tttcatgtag tagcccacga
     4381 agttgctcgc ccgcagaggg ttgcccgaca gccccaccac ggcaatcctg cgctggttga
     4441 gcgccccctg gatcacgtgg gggtcctgat accgttccat gtcgtgctcc gtcatgtcag
     4501 atgcccgcca gtccctgctc gagatcccag atgatgtcgt cggcatcctc gatgccgatg
     4561 gaaacgcgta tggtgcccgg accgatgccg gcggccgcca gttcctcgtc gctcagctgg
     4621 cggtgggtgg tcgtggccgg gtggatgacc aggctcttcg cgtcgcccac gttcgccaga
     4681 tgggagaaca aggtgagctg ctcgatgaac cgctggcccg cctcgcgccc gcccggcagg
     4741 tcgaagcaga agatggctcc ggcgccgcgc ggcaggtagc gggcgacccg gtctcgatag
     4801 gggcttccgg gcaggccggg gtagctaacc cgctcgacgt cctcgcgacc gtccaggtag
     4861 gcggcgacgg cctcggcgtt cgcgacgtgc tggcgcatcc gcagcggcag ggtttccaga
     4921 ccctgagcga acaggaaggc gttgaacggg ctcatggcgg cgcccagatc ccgcagggtc
     4981 tcggcccgca gcttcatcag gtacccgtag gtgccgaagg tctcgtggaa ggccagcccg
     5041 tgataggcgg gggagggctc cgcgatcacc ggaaagcggc cgttggacca gtcgaactct
     5101 cctgaatcca ccaccaggcc gccgatggac gtgccgtggc cgccgaggaa cttggtggcc
     5161 gagtgcacga cgatgtcggc gccccactcc atgggtcggg tgaggtaggg cgtggcgaac
     5221 gtgttgtcca ccatcagcgg catgccgtga cggtgggcga gctcggctaa aggctcgatg
     5281 tccagaacgt tgccgccggg gttgccgatg gtctcggcaa acagcagctt ggtgtcgggt
     5341 tgcagggccg cctcccaggt atcgatgcgg tcggggtcca cgaaggtgag cgccacgctc
     5401 atcttgcgga acaggtgctt gagctgagtc accgtgccgc cgtacagagc ggcggacgca
     5461 accacgtggt cgccgggctc gagcagggtg aagaaggcgg ccgactgggc ggccagcccg
     5521 ctggcaaagg ccaccgcgcc cgcgcctccc tccaggttgg cgatgcgctc ctccagggcg
     5581 gccacggtgg ggttcatgat ccgtgagtag gtgttgccgt attcctgcag gttgaaatat
     5641 gccgccgccg actcggagtc ctcgaagacg tagctgctcg tctggtagat gggcagcgcc
     5701 cgcgcgccgg tggccggatc aggccggctg cccgcatgca ccgaccgggt gtggaacccg
     5761 aactggtggg tgtcgtccgt agccggccgg tccgtggatt cgctcatgct gcctccccgc
     5821 agcgcgggct cgcgctgcct gctgcctgtg caggtaagat aacctatgcg ggccggccgc
     5881 gtcgcaacgc ctagcgtgcg ttctgacggg tctgctcgcc tggaattcat gggtcgacga
     5941 ggaaagcgat gagcgcgttc agagacgatg tgcgtgggtg gctggaggag aactgcccgc
     6001 cgagcatgcg cacacccctg ccggcggacg agtatcccgg cggcggccgg cgtgccgtct
     6061 acggcaatcc ggacaccggc gtgtggatgc agcggatggc ggaacggggc ttcaccgtgc
     6121 cgctgtggcc caccgagtac ggcggggcgg ggctcgacgc cggcgagaac caggttctgc
     6181 aggaggagct gcgccgcatc aacgcgcggc ccgcgctgct gggcatgggc atcagcatga
     6241 tcgggcccgc gctgctcgag ttcggcaccg aggcccagaa gcgcgagcac ctgccgaaga
     6301 tcgcccgggg cgagatctgg tggtgccagg gctacagcga gcccaacgcc ggctcggacc
     6361 tggccggcct gggaacgtcc gcggtggaag acggcgacga ctacatcgtc aacgggcaga
     6421 aaatctggac ctcgggagcc gaccgcgcgg actggatctt ctgcctggtc cggaccgatc
     6481 cgagcgcgcc caagcacgag ggcattacct tcatcctgtt cgatatggcc tcgccgggcg
     6541 tgacggtgaa gcccatcgag ctgatcagcg gctactcgcc gttctgcgag accttcttcg
     6601 acaacgtgcg tgcctcgcgg cgcaacgtgg tgggggagcc caacaagggc tggacagtcg
     6661 ccaagcgcct tttgcagtac gagcggacct caatcggcgg gatcggcggc tccagccaga
     6721 aggcggcgac cctcgaggag attgcccggg aggcgctggg caccgtggac gggcggattg
     6781 ccgacgccga cctccgggct ggcatcctgc gccatcgcat gaacgaccgg gccttcggcc
     6841 tgaccatgaa gcgctcggtg cagcagacgg cggccggcat cgcccccggc gccgagtcca
     6901 gcttcttcaa gtactacgga accgagcaga acaagggccg ctacgagctg ctgatgtccc
     6961 tgctcggcac gcgtggcctg ggctggagcg gtcccgggtt cagcgacgag gagcgcgacg
     7021 ccacgcgcgc gtggctccgg tcgaaggcca actccatcga gggcggcacc tcggaggtgc
     7081 agctgaacgt catcgccaaa cgaatcctcg gcctgcccga ctgacgtggc gctgggcgac
     7141 cgccattacc gctgggtagt cgtcggctac accctggtga tacagggggt cagcatcggg
     7201 atgctggtct acagctttgc gctcttcgcc attccctggc tcgacctctt cgccgccccg
     7261 cgtcgcgacg ttatgctcac cgtggcgatc ctgcagctgg tcaacggcgc ggtcagccct
     7321 ctggcgggcc gtgccatgga tcgatactcc atccgaacca tcgtgctggc gggcctggcg
     7381 ctgatgctcg tcgggctcgt cgtggtttcc cgggctcagg ccctgtggca ggtctggctc
     7441 gtctacgcga cgctcttccc cgtctccatg gccatgatga gcacgctggc ttcccagacc
     7501 ctggtgactc gctggttcga ctcgcgccgc ggggtcgcca tcggcatatc ggccacgggc
     7561 accaacgtcg gcggcatcgt tttcccgttg ctgattgccg gctggctgac ggacttcggc
     7621 tggcgggaga ccttcctgtg gctggcggtc accgctgcgg tggtcgtggg tcccctgagc
     7681 tggttcgtgc tcgggcgaac gccggccgga catcatccgg ccacccgcac gaccccgggc
     7741 ggtggcagcg tctggaccac ccgggcgatt ctttcaaccc gacgcttctg gattcccggg
     7801 ctcgccatcc tgccgctgaa cctggccttc ggggccgtgc agtacaacct gggggtgctc
     7861 agccgcgatc tggggctggc aaccgggacg gcggcgaacc tcatcgcctt gcacgccgtc
     7921 tgcatgatcg ccggcaagtt tttcttcggc agcctgggcg accgcattga tcaccgttat
     7981 ctgttctggc tgtccgccgg gctcatggtc gccgccctgc tggtgctgca gggcgagacg
     8041 cccgtgtgga tgcttgtcac cggcatcgtc atgatgggcc tggccggggg cgggatcctg
     8101 cccatgttcg gcatggtgtt cggcgcgcgt ttcggcgtgg ccgccttcgg ccgtgtgatg
     8161 gggctcgtca tgaccaccat gaccatcggc gccatgggtc cgctgctggc gggatgggca
     8221 tacgacctca cgggcagcta cgacgcggct tttctgggca tggcgctgct gctgcttccc
     8281 gctgcggtcg ccatgatctg gctgccgcaa cccggaaccg acacggcgtc ctgacccgtg
     8341 cgcatgcatt tcggccggtg ccggttgcat gtgagatccc ccgcggttta atttcagcgg
     8401 ccaggggccg tcgaaatttt tcttggggga agcatgattc gcatcacttt tctgctgcgc
     8461 aggaagccgg agctgtcggc cgaggagttc tatcgttact ggcgcgagga gcacggcccg
     8521 ctggtggcga gtcacgcaag ccggctgaac accctgcgct acgtccaggt ccacaccatc
     8581 gacgaccccg ccaatcaggc catggccacc gcccggggcg gcatggaagc gccgtacgac
     8641 ggcgttgccg agatgtggtg ggagagccgt gcggcgctcg tggcctctct ggaaaccaaa
     8701 ggcggccggc gggcgggcgc cgagctggtg gaggacgagg cgcgtttcat cgatctgccc
     8761 aactcgccca tgtggctcgg ccacgagtac ccccaggtga acccgacgcc ggagaatctg
     8821 gtcgcccggg agcgcgacac cctggtgaag ctgtacttcc cgctacgacc ggttcagggc
     8881 ctggacgagc gcgacgccca gagttactgg tacagcaacc acgggcccat catccggcga
     8941 caggccgccg gtgtcggcat ccagcgctac gttcaggtcc accgggtggc ggacgagctg
     9001 gaggccgggc tgcgcaaagc caggggtacc caggtggagg gttacatggg ccacgccgag
     9061 ctgtggttcg accgcaccgt gctcggcgtt tccacgccgg agcggcgggc ggccaacgag
     9121 cgcgccatcg ccgacgagag caacttcatc gacttccggc gctcgtgcat gtggctggcc
     9181 aaggagcacg tcttcgtcga ccgccggtga cggcgacgcg ggtggctcag gcgttgccca
     9241 cctcgtgctc gatgatgtcc gcgtacttgg ccagggccgc ttccttgcgc ctgatcaggt
     9301 gatattccgg gaacaggtcg tcggtaccct ggtactgccc gagcacctgc ttcgccacgg
     9361 tcaccttgtg aacctcggtg ggcccgtcgg ccagccccat gtggaacgag tccagcacgc
     9421 cggcagcaaa gggcatctcc gtggacaggc ccagggagcc gtgcacctgc agcgcgcggg
     9481 catagacgtc gtggtagacc ttgggcatct gggccttgac ggctgagatg tccgcgcgga
     9541 cttccttgta gttcttgagc ttgtcgatct tccacgcggt gcgcagcacg aacaggcgga
     9601 actgctcgat gtcgatccag gagtccgcga tcatggcctg gacgagctgc ttctgcgcca
     9661 gcacttcccc ctgggtttcc cgcgacaggg cccgctcgca catcatgtcg aaagcgcggc
     9721 gcgccttgcc catggtgcgc atggcgtggt gaatgcggcc gccgccgagc cgcacctgag
     9781 agacgatgaa tccctggccc ggctccccga gcatgtggtc ccgggggatg cgcacgtcgt
     9841 tgtagcggat gtaggcgtgg gcccccttgg actcgtagcc cacggccacg tggcggacga
     9901 tctcgatgcc ggccgtctcc gtgggaacga tgaacatgga catgcgctga taggggggtg
     9961 cctccgggtc ggtcaccgcc atcacgatga ggaacgacgc gtagcgggcg ttggaggaaa
    10021 accacttctc cccgttgatc acccactcgt caccgatgag ctccgcgcgg caggtgaaca
    10081 ccttgggatc ggcgccgccc tgaggttccg tcatggagta acaggagcag atctcgttgt
    10141 tcagaagcgg ctccaggtag cggtccttct gctccggcgt gccgtagtgg gcgaggatct
    10201 ccgcgttgcc cgagtcgggg gcctggcagc cgaacaggct cggcgcccag ccgctgcggc
    10261 ccaggatctc gttcatcagc gcgagctgca gctggcccca gccgccgccc ccgagcgcgg
    10321 gcgtcaggtg gcaggcccac aggccctgtt ttttcacctc ctgcttgagc ggcgccatga
    10381 tcgtcatcag cgcctc
//
LOCUS       DBNHKV010000030         5055 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1350442, whole genome shotgun sequence.
ACCESSION   DBNHKV010000030 DBNHKV010000000
VERSION     DBNHKV010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1350442"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="AC16PP_00850"
     CDS             complement(<1..544)
                     /locus_tag="AC16PP_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR6480197.1"
                     /translation="MDPTTLLLLLALLFGLYMAWGIGANDVANAMATSVGARAITVKQ
                     AILIAAVFEFLGAFLAGGEVTSTIRKGIIDSSALVGDPDVLILGMLAALLAAAVWLTV
                     ASRFGWPVSTTHSIVGAIVGFAIATIGMEAVHFDKVGSIAASWVVSPLLAGTLSFLLV
                     RSVQWLILDRPNPELYARRYV"
     gene            complement(598..1272)
                     /locus_tag="AC16PP_00855"
     CDS             complement(598..1272)
                     /locus_tag="AC16PP_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HIR6480198.1"
                     /translation="MANYFEGIFGRSPFKPVQEHMDLCHQAAALIEELLARAGSQSWP
                     EVEEIREQIVALERRADDRKRDIRLHLPRGFFLPVARADLLDLLARQDKIANKAKEIA
                     GLIVGRRMSFPPAIQESALELTRTSISACQRALDVVNELDELIATGFRGVEVQRVEEM
                     IDALDESESRNDDAQVRLRAELHGLERELDAVDVIFLYRIIDRIGDLADNAQSVGHRL
                     EMMLAR"
     gene            1420..2214
                     /locus_tag="AC16PP_00860"
     CDS             1420..2214
                     /locus_tag="AC16PP_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6480199.1"
                     /translation="MASITEFAVRDHVAYITLNRPEALNSLNPELRWTLSRHFDEVQS
                     NDDIWLAVVTGAGERAFCAGADLKHRARERDASAEQRAEWARMMEETTPLNQRWYLAK
                     PVIARVNGFALGGGLELALSCDIIVAAEHAELGLPEPRRGLIAGGVGVHRLPRQIGLK
                     AAMGYLLTGRHMSAQRAYELGLVNQVVPGAGLDAAVDEWVRDILRCAPLAVRATKEAA
                     MRGLDLPLPQAFYTEYEWERRRRGSDDALEGPRAFAEKRPPEWRGR"
     gene            2257..3507
                     /locus_tag="AC16PP_00865"
     CDS             2257..3507
                     /locus_tag="AC16PP_00865"
                     /inference="COORDINATES: protein motif:HMM:NF015557.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR6480200.1"
                     /translation="MPIELLLIVCLVVIFLVPIFFDVNVGIVAFITAFLVGALAMDLS
                     ARQVLAGFPDNMFVMLVGITLLLAIAHQNGTVDWIVAKLVRAARGRLVLLPWVLFVTA
                     LVTASLGPVAAPVLFVIGVGFVSRYGLNPLMVAAMIIHGTQSGAYSPIAPYGVVIGEL
                     AATAGIGYSPVAVYAGVVTFHVLLAAFVFLMLGGRALAGVRVEVAIGETAESERLDRQ
                     RTLTLIGFACLVLGLLVYQINLGFLAMSIALVLLLTGPREDRQAAVGNIAWPVVLVIA
                     GVLTYVGLLQHAGATDWLALQAKAMGSPLLMGLVLCFIVAVVTGVASTIGTIGILVPL
                     SAPFVLAGDLEATGLLAAMAVSAAVSDISPYSTWGAMFLATAGQVLNRQEILQAQLKY
                     TIVMVALMPILAWLVFVVPGWQVG"
     gene            3530..4036
                     /locus_tag="AC16PP_00870"
     CDS             3530..4036
                     /locus_tag="AC16PP_00870"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR6480201.1"
                     /translation="MSESLDKALTVARDRVVRFHYVIRDEAGSELESTREGEPLAVLQ
                     GRGSVLRGVEEALEGRNAGERLEVTLPPEQAFGARREDWTQRVPKKYLPDAKRLKPGD
                     AAVLRTREGPRPVTVVKVGSKVVDVDLNHPRAGMTLTFDLEIVEVREATPEEIAHRHV
                     HGPGGHRH"
     gene            complement(4046..>5055)
                     /locus_tag="AC16PP_00875"
     CDS             complement(4046..>5055)
                     /locus_tag="AC16PP_00875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR6480202.1"
                     /translation="PAMARPNLEVLTNAHVQHLEIETTDAGLRASGVTVQMGDDAPTT
                     ITARREVVLAAGAIGSPQILQLSGVGPGDLLHEHGIRVIHDLPGVGENLHDHLQVRSV
                     YKVHGTVTLNQRANSLIGKALMGLEYFTRKTGPLTMPPSQLGAFAKSDPSVASADIEW
                     HVQPLSLDAFGEPLHRFNAITPSVCNLRPTSRGHLRIKSPDPADYPAITLNYLATEHD
                     RQVILAGMRLTRRIMAAKALERFQPEEWKPGPSLTTDEELLTAAGDLGTTIFHPVGSC
                     RMGSDPMAVVGDRLAVHGVEGLRVIDASVMPQITSGNTNAPTVMIAEKGAEFMRADAA
                     S"
BASE COUNT          788 a         1617 c         1748 g          902 t
ORIGIN      
        1 gaacgtagcg ccgggcgtac agctcggggt tcgggcggtc caggatcagc cactgcacgg
       61 accggaccag cagaaacgag agcgtgcccg ccagcagcgg ggagacgacc cagctggccg
      121 cgatggagcc gaccttgtcg aagtgaacgg cctccatccc gatggtggct atggcaaagc
      181 cgacgatggc gccgacgatg gagtgggtgg tggagaccgg ccagccgaac cgcgaggcca
      241 ccgtgagcca caccgccgcg gcgagcagcg cggcgagcat tcccagtatc agaacatccg
      301 gatcgcccac cagagcactc gagtcgatga tgcctttacg gatggtggac gtgacttctc
      361 cgccggccag aaaagcgccc agaaactcga acaccgcggc gatcagaatc gcctgcttca
      421 cggtaatggc ccgggcgccg acggaggtgg ccatggcatt ggcgacgtcg ttggcgccga
      481 tgccccaggc catgtagagg ccgaacagca gcgcgagcag cagtagcagt gtcgtcgggt
      541 ccatcgccat ccctttcccg gtttttctgt cggctcagcg ccaggaagct tgcgccctca
      601 acgcgccagc atcatctcca gcctgtggcc gacgctctgg gcgttgtcag ccagatcacc
      661 gatgcgatcg atgatgcgat acagaaatat cacgtccacg gcatcgagtt cccgctccag
      721 accatgcagt tccgctcgga ggcggacctg ggcgtcatcg ttgcgggatt cactttcgtc
      781 gagggcgtcg atcatctctt ccacccgctg cacctctacg cctctgaagc ccgtcgcgat
      841 gagctcgtcg agctcgttga ccacgtccag cgcacgttgg caggccgaga tggacgttcg
      901 ggtaagctcc agagccgatt cctgaatggc cggcggaaaa gacatgcgcc gccccacgat
      961 gagcccggca atctccttgg ccttgttggc gatcttgtcc tggcgcgcca gcaggtccag
     1021 caggtcggcg cgcgcaaccg gcaggaagaa gcctcggggc aggtgcaggc ggatgtcccg
     1081 cttgcggtcg tcggcccggc gttccagggc gacgatctgc tcgcgtatct cctccacctc
     1141 gggccaggac tgggatcccg ccctggccag cagctcttcg atgagcgccg cggcctgatg
     1201 gcacagatcc atgtgttcct gtacgggctt gaaaggcgag cggccgaaga tgccctcgaa
     1261 gtagttcgcc acggatatgt ctcccttccc gcggcgaaaa ctaacccacg tggctgggca
     1321 aagggaagag gacattcggt cttcttgccg attgccgggc tctgatttcc acgttagatt
     1381 gaccggttac tcgtctgggt gagttcgggg aaggggacga tggcatcgat taccgagttc
     1441 gcggtgcgcg atcacgtggc gtacattacc ttgaaccgcc ccgaggcgct caactccctg
     1501 aaccctgagc tgcgctggac gctctcgcga cacttcgacg aggtgcagag caatgacgac
     1561 atctggctgg cggtggtgac cggagccggc gagcgggcgt tctgcgccgg cgccgacctc
     1621 aagcaccgcg cccgcgagcg cgacgccagc gccgagcagc gcgccgaatg ggcgcgcatg
     1681 atggaagaaa cgacccccct caatcagcgc tggtacctgg ccaagcccgt catcgcgcgg
     1741 gtcaacggct tcgcgctggg cggcgggctg gagctggcgc tgtcctgcga catcatcgtt
     1801 gcggccgagc acgccgagct cggcctgccg gagccccgac ggggactgat cgccggcggc
     1861 gtgggcgtgc atcggctgcc gcggcagatc ggcctgaaag cggccatggg ttacctcctc
     1921 acgggccggc acatgtcggc ccagcgggct tacgagctgg gcctggtaaa ccaggtggtg
     1981 cctggggcgg ggctcgacgc ggcggtggac gagtgggtga gagatattct gcgctgcgcg
     2041 ccgctggccg ttcgggccac caaggaggcc gccatgcgag gcctcgacct gccgctgccc
     2101 caggcctttt atacggagta cgagtgggaa cggcgccggc ggggcagcga cgatgctctg
     2161 gaagggccca gggcctttgc cgagaaacgt ccgcccgagt ggcgggggcg ctgagcctgc
     2221 gagccatcgt gcggccgatc tggcagcgtt gacgtcatgc ccatagagct cctgctcatc
     2281 gtctgcctcg tcgtcatctt cctggtgccg atcttcttcg acgtcaacgt gggcatcgtg
     2341 gccttcatta ccgccttcct ggtcggggcg ctggccatgg acctgagcgc gagacaggtg
     2401 ctggccggtt ttcccgacaa catgttcgtc atgctggtgg gcatcacgct gctgctcgcc
     2461 atcgcccatc agaacggcac cgtggactgg atcgtcgcaa agctcgtccg agccgcccgg
     2521 ggacgactgg tgctgctgcc gtgggtgctc ttcgtcaccg cgctggtcac cgcatctctc
     2581 gggcccgtgg ccgcgccggt gctgttcgtg atcggcgtgg ggttcgtctc ccgatacggg
     2641 ctcaacccgc tgatggttgc cgccatgatc atccacggca cccagtccgg cgcttactct
     2701 cccatcgcgc cctacggggt ggtgatcggc gagctcgctg ccaccgctgg gatcggctac
     2761 tcgcccgtgg ccgtctacgc aggcgtggtg acctttcacg tgctgctggc cgccttcgtg
     2821 ttccttatgc tgggcgggcg ggccctggcc ggcgtgcggg tagaggttgc cattggtgaa
     2881 accgccgagt cagagcggct cgaccgccag cgcacgctga ccttgatcgg ttttgcctgt
     2941 ctggtgctgg ggctcctggt gtaccagatc aacctgggct ttctcgccat gagcatcgcg
     3001 ctggttctgc ttctcaccgg tccccgggaa gaccgtcagg cggcggtggg caacattgcc
     3061 tggcccgtgg tgctggtgat cgccggcgtg ctgacctacg tgggtctgct gcagcatgcc
     3121 ggcgccaccg actggctggc ccttcaggcc aaggcaatgg gctcgccgct gctcatgggc
     3181 ctcgtgctgt gcttcatcgt ggcggtggtt acgggggtag cgtccaccat cggcaccatc
     3241 ggcattctgg tgccgctatc cgcacccttc gtccttgccg gcgacctcga agccacgggg
     3301 ctgctggcgg ctatggccgt ctccgccgcg gtttcggaca tctctccgta ttccacctgg
     3361 ggcgcgatgt tcctggctac ggctggtcag gtgctgaacc gccaggagat cttgcaggcc
     3421 cagctcaagt acaccatcgt catggtcgcg ctgatgccca tcctggcgtg gctggtgttc
     3481 gtggtgcccg ggtggcaggt gggatgagaa gtctatcggg gagaaagtga tgtcggagtc
     3541 tttggacaag gccctcaccg tggcgcggga tagggtggtg cgctttcact acgtgattcg
     3601 cgacgaggcg ggctcggagc tcgagtccac gcgcgaaggc gagcctctgg ccgtgctgca
     3661 ggggcgcggc agcgtgctgc ggggggtcga ggaagcgctg gagggccgaa acgccggcga
     3721 gcgcctggaa gtaacccttc ctcccgagca ggctttcggc gcccggcggg aagactggac
     3781 ccagcgcgtt cccaagaaat atctgcccga tgccaaacgg ctcaaacctg gcgatgcggc
     3841 cgtgctgcgg acccgggagg ggccgcgtcc ggtcaccgtg gtgaaggtgg ggagcaaagt
     3901 cgtggacgtg gatctcaacc atccccgcgc ggggatgacc ctgacgttcg atctcgagat
     3961 cgtggaggtg cgcgaagcca cgcccgagga gatcgcccac cgccacgtgc acggccccgg
     4021 gggccatcgg cactgaggcg gtgtctcagg aggccgcgtc ggcccgcatg aactccgcgc
     4081 ccttctcggc gatcatcacc gtgggcgcgt tggtgttgcc ggaggtgatc tgcggcatta
     4141 ccgaagcgtc gatcacccgc agcccttcaa cgccgtgcac ggccagccgg tcgccgacca
     4201 cagccatggg gtcgctgccc attctgcagc tgcccaccgg gtgaaagatg gtggtgccga
     4261 gatcgccggc ggcggtgagc agctcctcgt cggtagtcag gctggggccg ggcttccact
     4321 cctccggttg aaaccgctcc agtgcctttg ccgccatgat ccggcgggtg agccgcatcc
     4381 cggcgaggat gacctgacgg tcgtgctcgg ttgcgaggta gttgagggtg atcgccggat
     4441 aatcggcggg gtccgggctc ttgattcgca gatgcccgcg actcgtgggc cgcaagttgc
     4501 acaccgacgg ggtgatcgcg ttgaagcggt gcagcggctc cccgaaggcg tccagcgaca
     4561 gcggctgaac gtgccactcg atgtccgcgg aggcgaccga cggatcggac ttcgcgaacg
     4621 cgcccagctg agagggcggc atggtgagtg gtccggtttt cctggtgaag tactccagcc
     4681 ccatcagcgc cttgccgatg agggagttcg cccgctgatt caacgtcacc gtgccgtgca
     4741 ccttgtagac ggagcgcacc tgcaggtggt cgtggaggtt ctcccccacg cccggcaggt
     4801 cgtggatcac ccggatgccg tgctcgtgca gcagatcgcc ggggccaaca ccggagagct
     4861 gcagaatctg gggggagccg atggcgcctg cggcaagcac cacttcccgg cgcgcggtga
     4921 tggtggtggg cgcgtcgtcg cccatctgca cggtgacgcc ggaggcgcga aggccggcgt
     4981 cggtggtctc gatttccagg tgctgaacgt gggcgttcgt gagcacctcg agattgggac
     5041 gcgccatggc cgggc
//
LOCUS       DBNHKV010000031         3453 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_218120, whole genome shotgun sequence.
ACCESSION   DBNHKV010000031 DBNHKV010000000
VERSION     DBNHKV010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_218120"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1438)
                     /locus_tag="AC16PP_00880"
     CDS             complement(47..1438)
                     /locus_tag="AC16PP_00880"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR6480203.1"
                     /translation="MADLLAEQLRLQPVSVTRRMSASLPGLRRLAQEKGLKIHHLGAG
                     YPHPEVTDPRSFIAHREAYFAHLEEREGLNDPRALPEYLRESFSYTDTLGPAGTRAAF
                     ARVYGRDWGVTMDPGRLIPTVGATGGINLACSMFERPGVPLAYITDAPTYAGFNARAT
                     LCQHAAIYSVPMDDEGPLPDVLRDQIRAARADGRFVPFYYTVPDGHNPAGFSFSQARR
                     EAILRVVREEGILILEDAPYVYINFAPAANRPRPFLAMDPRCTIHLFTGSKIGFPGPR
                     VGFLYSEAELAIAGGRTASLTEFALTEASADMLFQNPAALLGFEALLHDDDFSERRSL
                     WPVAETKLAVYRENRSIMLDGLERELGGHRDLFHWTDPKAGFFTVFTFLGPGIRTDDA
                     FVNAMVADHGVVVIPMYDFYPEDARRRDPDAGFDQLRLSFCFSESLGEQRRTDLEEAV
                     AAFCAAAKKLAGV"
     gene            complement(1499..2560)
                     /gene="rsgA"
                     /locus_tag="AC16PP_00885"
     CDS             complement(1499..2560)
                     /gene="rsgA"
                     /locus_tag="AC16PP_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR6480204.1"
                     /translation="MNGARPTLPELGWTPFFAEQLSPEEADRLIPARVFGVQRTHITV
                     MAEEGELSMPLGGRWFQLDPQDRPTVGDWVLVSEQRDSVVRLLERRSLLKRLSPGRTG
                     AVQLIAANVDTLFLVTSCNQDFNPRRLERYLALAYESGIEPVVVLTKADLCDDLERYV
                     DGARRIVPGLEVVALDARDRAQLAPLASWLATGRTVALLGSSGVGKSTLVNTLSEAQV
                     QRTAQIREDDGKGRHTTSHRSLHPVPGGGLMLDSPGMRELGMADAELGVRQMFEDVEA
                     LAHQCRFADCAHDSEPGCAVKAALEAGELTEERLESYRKLSREEAVNAETIAERHARS
                     RATDKLHRRVQDTSPKRRS"
     gene            complement(2567..3322)
                     /locus_tag="AC16PP_00890"
     CDS             complement(2567..3322)
                     /locus_tag="AC16PP_00890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR6480205.1"
                     /translation="MTIRLEIDGIEVELRRNSRRRTRIGMAFDPSGFVIMDAPPNVGE
                     NEVREVVAEHHRWLRVRLDKARDAVHEVRALNYVAGELVHYLGQAYRLEVRAGGEVRV
                     ELSAPAHSQLPLFADVAFGTLQVTTPEQSPDGVKTAVCDWYSAQGDRVFAERLAGWSE
                     LPWLNGTTPRWRHTFMRSQWGSCSARGKISLNTHLVKAPVSLVDYVILHELCHLEHHD
                     HGRRFYALMERHMPDWERRRAALDRFLPVLLHD"
BASE COUNT          596 a         1157 c         1168 g          532 t
ORIGIN      
        1 cgcgaagggt ggccccgaca ccctgaggcg acggagcctc ggcgtctcag accccggcga
       61 gcttcttcgc cgccgcgcag aaggcggcta ccgcttcctc gagatccgtg cgccgctgct
      121 cacccaggct ttcactgaag cagaacgaca accgcagctg gtcgaagccg gcatcggggt
      181 cgcggcgccg ggcatcctcc gggtagaagt cgtacatcgg gatcaccacc accccatggt
      241 cggccaccat cgcgttgacg aaggcatcgt ccgtgcggat tcccgggccg agaaacgtga
      301 aaacagtgaa gaagcccgcc ttggggtcgg tccagtgaaa cagatcccga tgcccgccga
      361 gctcccgttc caggccgtcg agcatgatcg accggttctc gcgatacacg gcgagcttcg
      421 tctccgccac cggccacaac gaccgccgct cgctgaagtc gtcgtcgtgc agcagcgctt
      481 cgaagcccag cagggcggcc gggttctgaa acagcatgtc ggcgctggct tcagtcagcg
      541 caaattcggt cagcgaagcc gtccgcccgc cggcaatggc aagctcggct tccgagtaga
      601 gaaagcctac gcgcggcccg ggaaagccga tcttggatcc ggtgaagagg tggatcgtgc
      661 agcgcgggtc catggcgagg aagggccggg ggcggttcgc ggccggcgca aagttgatgt
      721 agacgtaagg agcgtcctcg agaatgagga tgccctcctc cctgacgacc cggagaatgg
      781 cttcccgccg tgcctggctg aacgagaacc ctgccgggtt gtgcccgtcc ggcaccgtgt
      841 agtagaacgg cacgaagcgg ccgtcggccc gcgcggcgcg gatctgatca cggagaacgt
      901 ccggcagcgg gccttcgtca tccatgggca cgctgtagat ggcggcgtgc tgacacagcg
      961 ttgcacgggc gttgaatccg gcgtaggtcg gggcgtcggt gatgtaagcc agcggcacgc
     1021 ccgggcgctc gaacatggag caggcgagat tgatgccgcc ggtcgccccc accgtcggta
     1081 tgaggcgccc gggatccatc gtgactcccc agtcccggcc gtaaacgcgg gcaaacgccg
     1141 cccgcgtgcc ggccgggccc agcgtatccg tgtaggagaa cgactcccgc aggtactcgg
     1201 gcagggctcg agggtcgttg aggccttccc gctcttcgag gtgggcgaag taggcttccc
     1261 gatgggcaat gaacgaccgg ggatcggtca cttcaggatg gggatacccc gctcccaggt
     1321 ggtggatctt caaccccttc tcctgagcga gcctgcgcag gccgggcaaa gaggcagaca
     1381 tgcgacgggt gacggaaacg ggctgcaggc ggagctgctc ggcgagaagg tcggccatga
     1441 cgcgcctcgg gcggaagagg cgcgtacttt aaggcatctc cggctcccga ggtaggcgtc
     1501 agctgcggcg cttgggagat gtgtcctgga cccgccggtg cagcttgtcc gtcgctcggg
     1561 agcgggcgtg acgttcggca atggtttccg cgttcacggc ttcttcccgg ctgagcttgc
     1621 ggtaactctc cagccgctcc tcggtaagct ccccggcctc gagggccgcc ttgaccgcgc
     1681 agccgggttc cgagtcgtgc gcgcagtcgg caaagcggca ctggtgcgcg agcgcttcca
     1741 cgtcctcgaa catctgacgc acgccgagtt ccgcatccgc catgccgagc tcccgcatac
     1801 ccgggctgtc cagcatcagc ccgccgccgg gcaccgggtg cagcgagcgg tgcgaggtgg
     1861 tgtgcctgcc cttcccgtcg tcttcccgga tctgcgccgt gcgctgcacc tgagcctcgg
     1921 agagggtgtt gaccagcgtg gatttaccca cacccgacga gccgagcagc gccaccgtgc
     1981 ggccggtcgc caaccaggac gcaaggggag cgagctgtgc ccggtcgcga gcgtccagag
     2041 ccaccacctc gaggcccggg acgatccgtc gggccccatc cacgtagcgc tccagatcgt
     2101 cgcatagatc cgccttggtg agcaccacca cgggctcgat gcccgactcg tatgccagcg
     2161 ccaggtaacg ctcgaggcgg cgcggattga aatcctgatt gcacgaggtg accaggaaca
     2221 gcgtatcgac gttggccgcg atgagctgca ccgcacccgt gcggcccggg ctcaatcgct
     2281 tgaggaggct tctgcgttcg agcagcctca ccacgctgtc gcgctgctca gaaaccagca
     2341 cccagtcgcc caccgtgggc cggtcctggg gatcgagctg aaaccagcgc ccgcccaggg
     2401 gcatggaaag ctcaccctcc tcggccatca ccgtgatgtg agtccgctgc acgccgaaaa
     2461 cccgggcggg gatgagccgg tctgcctcct cgggagacag ctgctcggcg aagaacggtg
     2521 tccagccgag ctcggggaga gtgggcctgg cgccgttcac gagagatcag tcatggagaa
     2581 gcaccggcag gaagcggtcc agggcggcgc ggcggcgctc ccagtcgggc atgtgccgct
     2641 ccatcaacgc gtaaaagcgc cgtccgtgat cgtgatgctc gagatggcac agctcgtgaa
     2701 gaatcacgta gtcgacgaga gacacggggg ccttgaccag atgggtgttg agcgagatct
     2761 tgccccgcgc gctgcagctg ccccactggc tgcgcatgaa ggtgtgccgc cagcgcggcg
     2821 tcgttccgtt gagccaagga agttccgacc accccgccag ccgttcggcg aagacccgat
     2881 cgccctgtgc cgagtaccag tcgcacacgg cggttttgac cccgtccggc gactgctcgg
     2941 gcgtcgtcac ctgcagggta ccgaacgcca cgtcggcaaa cagcggcagc tgggaatggg
     3001 caggcgcgct gagctccacc cgcacctccc ccccggcgcg aacctcgagc cggtacgcct
     3061 ggccgagata gtggacgagc tcgccggcaa catagttgag cgcgcggact tcgtgaaccg
     3121 cgtcgcgcgc cttgtcgagc ctgacgcgca gccagcgatg atgctcggcg accacctccc
     3181 ggacttcgtt ctcgccgacg ttggggggcg cgtccatgat cacgaatccg ctggggtcga
     3241 aagccatgcc gatacgggtg cgacgccggg agttgcggcg cagctccacc tcgatgccgt
     3301 cgatttccag ccgaatcgtc aaaagtccca ggtcgttctg gccggcggca ggcagagagc
     3361 ctcgtccagc cggccgagca gctcggtcgg gcccgtcagc tcgtcgctca gagcaagagc
     3421 cgaagcgccg accaccccga gccacaatct cgt
//
LOCUS       DBNHKV010000032        11623 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_713971, whole genome shotgun sequence.
ACCESSION   DBNHKV010000032 DBNHKV010000000
VERSION     DBNHKV010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11623)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11623)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11623
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_713971"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="AC16PP_00895"
     CDS             complement(<1..283)
                     /locus_tag="AC16PP_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480206.1"
                     /translation="MSDVAIVGIGMHPFGRHEGVTGMEQGASAVREACRDAGVEWKDI
                     QFAFGGSSAAGAPDTMVSQLGLTGLQFINVQNGCATGGSALFSAYNTIMS"
     gene            complement(295..720)
                     /locus_tag="AC16PP_00900"
     CDS             complement(295..720)
                     /locus_tag="AC16PP_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6480207.1"
                     /translation="MTQVSINDNVLELSAEGPRLKGMRCKTCGNHVFPFQKGCPKCAG
                     TDVEPVDLDTRGTLWAWTIQGFPPKAPPYLGEADPEKFKPYGVGYVEIPGQTKVEARL
                     TESDPTKLKEGMEMELTVEPISKDEDGNDIVTYAFKPVE"
     gene            complement(737..1873)
                     /locus_tag="AC16PP_00905"
     CDS             complement(737..1873)
                     /locus_tag="AC16PP_00905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6480208.1"
                     /translation="MVLVLSEEQELLRDSARDFAGNAIEVDWLRKVREQGAAGFDRDV
                     WRQMAELGWTAIPFAEEVGGLGLGYAEAGVVLEELGKALAVTPYLSSVVLGGGAVDLG
                     GSAEQKAEILPGVCDGTTLLTLAYQETARHDPYAIETSAVKTDGQWVLNGRKQLVLDG
                     GSADRIVVAARSSGAPGDRSGLTLFLVDAGAAGLDVKPGLLLDSRSAAHVALNDVTVG
                     EDAVIGEVDGGAEILDRVFERAAVALSAEMLGGIQEAFDRTVEYLKEREQFGAKIGSF
                     QGLKHRAARWFCEVELTRSIVMQALRAIDDGAENLAEIVSTCKARASDAFRLSGQEGI
                     QMHGGMGVTDEADIGFFLKRARVSELLLGDSAFHRNQFAELKGY"
     gene            complement(1879..3063)
                     /locus_tag="AC16PP_00910"
     CDS             complement(1879..3063)
                     /locus_tag="AC16PP_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6480209.1"
                     /translation="MSDLEAFRLETREWLEANCPPGVRNRPGEVVWGGRRCQFPNEDA
                     KIWLERMVEKGWTVPTWPKPYGGAGLNDEEAKVLRQEMARIEANAPLFSFGIAMLAPA
                     LMEFASEEQQLEHLPPIARGDFRWCQGYSEPGAGSDLASLRCRAVRDGDHYVVNGSKI
                     WTSDADVSDWIFCLVRTDPDVPKHEGISFLLIDMETPGVSVTPIELISGASPFCQTFF
                     DDVRVPAANLIGEENKGWTIAKRLLQHERAMLGETATRRGGANRKGLVEVARDALRAP
                     DGPLPDAAVREEIAQFDMDRMCYGATFQRSADAARAGRGPGPETSMFKLYLSELNQRG
                     ADLRQRIVGADGLGWAGEGFDADELQITREWLYGRAATILGGTSEIQMNIIAKRVLGL
                     PD"
     gene            complement(3146..4003)
                     /locus_tag="AC16PP_00915"
     CDS             complement(3146..4003)
                     /locus_tag="AC16PP_00915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6480210.1"
                     /translation="MKIGAVFPHLEIGSDPAVLRDWAQTAEDLGYSHLLAYDHVLGAV
                     HAGREPALWGPYTEESAFHEPFVLFGYFAAVTRRVELVTGVIILPQRQTALVAKQAAE
                     IDILSGGRLRLGVGTGWNYVEYDSLGENFANRGKRQEEQIEVLRSLWAEPVIDYSGNY
                     HRIERAGLKPLPGRRIPIWLGGFSDVAYRRAARMGDGFIFGGSIKDGLAGLGKIRGFL
                     AEAGREQAGFGIEAMVNYGAGPDRWRADLEAWEAAGADYVSMRGMDAGLETPQDHIDA
                     LRRYWEAVS"
     gene            complement(4036..4812)
                     /locus_tag="AC16PP_00920"
     CDS             complement(4036..4812)
                     /locus_tag="AC16PP_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019408213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6480211.1"
                     /translation="MTPELVKTELRPDGIAVVTIDNPPVNAHSGQVLTEMAWTFDAIS
                     DQDEARVVVLTGAGKVFCAGADIKSRSGRAPEPGDHWQSSRAAREAYHSVRECVKPVI
                     GALNGPALGGGLAFAASCDMLIAAETACLGLPEIDVGLLGGGRHAQRLFGHSRLRRMM
                     LTGYRVYGPELYRLGIVEACVAPDALMDEALALAAEVAAKSPIAIRLAKHALNTIEEL
                     SLRDGYRFEQTMTGQLAKTEDSKEAMRAFVEKRKPQFKGR"
     gene            4944..6320
                     /locus_tag="AC16PP_00925"
     CDS             4944..6320
                     /locus_tag="AC16PP_00925"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6480212.1"
                     /translation="MSKSQPNQLLLTNARLFPGVSDEVFADGAVWIEGRTIRYAGPGC
                     DLPAVADDVPRVDLHGQFVMPGMTESHSHLSYSNNGPTELDKSPVEDAMIHAVANARL
                     MLGSGFTSAISFGSVHRIDVFLRDAIDAGRIPGPRLEASSRDVGATSSNADLHPDYFK
                     PQMEGLGMLADGPWAVRKAVRTIRKNRGNVVKLFLDGEGPGGHAPPGELTYTDEEVAA
                     AVDEAHRRGMRVACHSRSAAAVKQAVRFGIDFIGHANYLDDEALDMLKRSRERLFVGP
                     AIAWELAFLRHAAELGSPPGSQRYKFYEQEVEATKNAVRRLREAGVRVLIGGDYGLSI
                     TPHGTYAKDLQYFVELFGLSPAEALLCATRDGGAAVDPSGMLGTLEEGKLADLVIVDG
                     DPLEDITVVQDHDRITAVMKDGVVHRRLAVDNPYQASPDALQEELAARAADPERQRQA
                     AVLLESSG"
     gene            6364..7605
                     /locus_tag="AC16PP_00930"
     CDS             6364..7605
                     /locus_tag="AC16PP_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR6480213.1"
                     /translation="MNLQTLATPRIGWRWPAALILFLLGFAGFATGVAPSERPDVAQA
                     DLLTKAYYSLGLFVVGGLDIGTPASGPLLGRAMLWIAYFGAPLLTASAVIEALVRVMA
                     PHRWQMRRLHDHVVIVGSGELTISYLRVLRGSHPSTPVVVVDEHVDPVREQELAQAFD
                     VRVVIGNITHDFVQNGLRLDRAARVVLLGDSDFQSFEAASKMLRRFPHLESRLVLHCQ
                     RLRFLRSMEATTVGRLCITFNSYHLAAASLVKQQLIGHFEKTEHRDVVVLAGFGRFGQ
                     TILEELQNLARDELDTVAIIDVDADRRVMVAEEQRRLGGNYRRVVLQGDISHPEVWRS
                     LAASVDLSKDSPTVILGTGRAEDNLRTALWLKQSYPNAFVFARTNDKSELAVEVGSEH
                     GIRNISIRQLVEDNIPEEWLA"
     gene            complement(7615..8094)
                     /locus_tag="AC16PP_00935"
     CDS             complement(7615..8094)
                     /locus_tag="AC16PP_00935"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6480214.1"
                     /translation="MERTVKISQGRHAIEDIMYGYAEGIDTGNLETVAPLFAHGVMVM
                     PDGSELNGPEEVLRTFSDVIIFYDDEENVVPYQRGACSPRTKHVVTNSIFEFDNAVRN
                     ADVRSYFTVYQSLGGANEIIVGGRYIDQYELMIQGWVLVRREVYLENVGDLSRHLNG"
     gene            8226..9326
                     /locus_tag="AC16PP_00940"
     CDS             8226..9326
                     /locus_tag="AC16PP_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6480215.1"
                     /translation="MAKRLIVMAGLLALVFGSLFGWKAFERHKTAEYLANARPPAVVV
                     SSTRVEEDRWRQQVESVGSLSAIQGVDVSSEVPGTVADISFQSGQQISAGELLVQLDA
                     TAERAELRSLEAQLVLARLDYERALGLLERTMLSQAQLDRAKSVMDSLQAQVEEQAAF
                     IAKKAIRAPFAGELGIRQVNLGEYVSPGTPIVTLQALDPIYANFALPERFLRELAVDQ
                     PLRVVVAAYPEDEFLGTVTAISPKVEETTRNVVVQATIANADRRLRPGMFARITVQTG
                     GDHRVLTVPRTAITFNPYGESVFLINEQDGALLVDRRQVITGEIRNERVEVVSGLLAD
                     DLVVATGQLKLRAGQRVKIDNSIDLSRPVTGP"
     gene            9323..>11623
                     /locus_tag="AC16PP_00945"
     CDS             9323..>11623
                     /locus_tag="AC16PP_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6480216.1"
                     /translation="MNFTDTFVRRPVLASVISLLVLLVGIRAAGLLELREYPESNRAT
                     ITVTTAYPGADADLVQSFITVPLQRAISEAEGIDYLTSDSRQGVSIIEAFMELNYDPN
                     AAIAEIQAKVASQRNLLPQESMDPVIDSRTSLGWALMYLAFTSQEMSPAQVTDYLHRS
                     VIPRLQAIPGVAKAQINGNPTLAMRIWLNPHRMAALGVTATDVRAALQRNNFQAGVGE
                     TKGDYVRVTLSSTTGITAREDFARLIIRRDGETLIRLRDVAEVNLDSKNYDQTSWYNG
                     QPAILLALETSPGANPLTVSQAVRVLLPDIESQLPSNMEMQLAHDGSQYIDDSINEVL
                     RTLLEAMLIVLLVIFLSLGSWHAAVVPAVSVPLSLVGACALMLAFGFSLNLLTLLAML
                     LAIGLVVDDAIVVVENVHRHIAMNKTPFQAAIDGARELGLPILSMTTTLLAVYAPIAF
                     MGGLVGILFTEFAFSLAGAVLISGLVALTFSPMLSSKVLTSGEEPGRFESAVERFFAR
                     LAERYRARLSRALDYQPVTYLFAIVVMGSIYFMFTTSNTELAPTEDREVLNVQITAPE
                     TATLEYAVAHVRPAVKMFETFPEQRRTFLMIGGGGTPATSFGGFRFTPVAERERSQME
                     MQPILQRMLNEIAGVQIAVFSFPSLPGAARGTPLQFVIASDQDYRQLDTLADDVVQAA
                     YRSGNFLFLNKSVEFALPQTQIVIDRDRAGDLGIEMNDIGVALATLLGGNYVNRFSLA
                     GRSYEVIPQVERRFRASHEMLNDNYLR"
BASE COUNT         1969 a         4029 c         3649 g         1976 t
ORIGIN      
        1 cggacatgat ggtgttgtag gcggagaaga gcgccgaccc gccagtcgcg cagccgttct
       61 ggacgttgat gaactgcaaa cccgtgagac cgagctgcga gaccatcgtg tccggcgcgc
      121 cggccgccga gctgccgccg aaggcgaact gaatgtcctt ccattcaacg cccgcatccc
      181 tgcaagcttc gcggaccgct gacgcaccct gctccatccc ggtcacgccc tcgtgccggc
      241 cgaatgggtg catgcctatg ccaacaattg ctacgtcgct catggtttct ctccttactc
      301 gaccggtttg aatgcgtagg tgacgatgtc gttaccgtct tcgtctttgc tgatgggctc
      361 caccgtcagc tccatctcca tgccctcctt gagcttcgtc gggtccgact cggtcaaccg
      421 ggcctcgacc ttggtctgcc cgggaatctc gacgtagccc acgccatagg gtttgaactt
      481 ttccggatcg gcttcaccca gatagggagg ggccttgggc ggaaaaccct ggatcgtcca
      541 ggcccacagc gtgccgcggg tgtctagatc cacgggctcc acgtcggtgc ccgcgcactt
      601 cgggcagccc ttctgaaacg gaaaaacgtg gttgccgcag gtcttgcagc gcatgccttt
      661 gagccgcggc ccttccgccg acagctccag cacgttgtcg ttgatggaaa cctgggtcat
      721 gtcacctcca gtcgggctaa tagcccttta actcagcaaa ttggttgcga tggaaggccg
      781 agtccccgag cagcagctcc gaaacccggg cgcgcttcaa gaagaagccg atgtccgcct
      841 cgtccgtcac gcccatgccg ccgtgcatct gtatcccttc ctggcccgaa agccggaagg
      901 catccgaagc cctcgccttg caggtgctca cgatctcggc gaggttttca gcgccgtcat
      961 cgatggcgcg cagcgcctgc atgacgatgg agcgggtcag ctccacctca cagaaccagc
     1021 gggccgcgcg gtgcttcaag ccctgaaagg aaccgatctt cgcgccgaac tgctcgcgtt
     1081 ccttgagata ctcgacggtc cggtcgaagg cttcctggat gcctccgagc atctcggccg
     1141 agagcgccac cgccgcgcgc tcgaagaccc ggtcgagaat ctcggcacca ccgtcgacct
     1201 cgccgatcac ggcatcctcg cccacggtca cgtcgttcag ggccacgtga gccgcgctgc
     1261 ggctgtcgag cagcaggccc ggcttcacgt cgagcccggc ggcgcccgcg tcgaccagga
     1321 acagggtcag ccccgaccga tcgccgggcg ccccgctcga ccttgccgcg accacgatcc
     1381 ggtccgcgct gccgccgtcc agaaccagct gcttgcggcc gttgagcacc cactggccat
     1441 cggttttcac ggcgctcgtc tcgatggcgt agggatcgtg acgggcggtc tcctggtagg
     1501 cgagcgtcag cagcgtcgtg ccatcgcaga cgccgggaag gatctcggcc ttctgctcgg
     1561 cactgccgcc gaggtctacg gcgccgccgc ctagcaccac gctggacagg tagggcgtca
     1621 ccgccagcgc cttgccgagc tcctcgagca ccacgccggc ctccgcgtag ccgaggccga
     1681 gcccgccgac ctcttcggca aacgggatcg ccgtccagcc gagctcggcc atctgccgcc
     1741 agacgtcccg atcgaaaccg gcggcgccct gttcccgcac tttccgcaac cagtcgacct
     1801 cgatggcgtt gccggcgaaa tccctggcgc tgtcccgcag cagttcctgc tcttcgctga
     1861 gtaccagaac cacggcggct aatccggcag gccaagcacg cgcttggcga tgatgttcat
     1921 ctgaatctcc gaggtcccgc ccaggatggt ggccgcacgc ccgtagagcc actcacgggt
     1981 gatctgcagc tcgtccgcat cgaacccctc gccggcccag ccgagcccat cggcgccgac
     2041 gatgcgctga cgcaggtcgg ccccgcgctg gttcagctcg gaaagataga gcttgaacat
     2101 cgaggtctcc ggcccgggac cgcggccggc tctggccgcg tcggcgctgc gctgaaacgt
     2161 cgcgccgtag cacatgcggt ccatgtcgaa ctgagcaatc tcctcgcgca cggcagcgtc
     2221 gggcagcggg ccatccggcg cacgcagggc gtcccgcgcc acctccacca gtcctttacg
     2281 gttggcgccg ccccggcgcg tggcggtctc gccaagcatg gcgcgctcgt gctgaagcag
     2341 ccgcttggcg atggtccagc ccttgttctc ctcgccgatg aggttggccg cggggacccg
     2401 cacgtcgtcg aaaaaggtct gacagaaggg cgaggcgccg ctgatcagct cgatgggggt
     2461 gacggagaca ccgggtgttt ccatgtcgat caggaggaag ctgatcccct cgtgcttggg
     2521 cacgtcggga tcggtgcgca ccaggcagaa aatccagtcg gaaacgtccg cgtccgaggt
     2581 ccagatcttg gagccgttca ccacgtagtg gtcgccgtcc cgcacggccc tgcagcgaag
     2641 cgacgcgagg tcggaaccgg cccccggctc gctgtagccc tgacaccagc ggaaatcgcc
     2701 gcgggcgatg ggcggcaggt gctcgagctg ctgctcttca gaggcgaact ccatcagcgc
     2761 cggggcgagc atggcgatgc cgaacgagaa cagcggcgcg ttggcttcga tgcgcgccat
     2821 ctcctggcgc agaactttcg cctcctcgtc gttcaggccg gccccgccat aaggcttggg
     2881 ccaggtgggc acggtccagc ccttctcgac catgcgctcg agccagattt ttgcatcctc
     2941 gttcggaaac tggcagcggc gcccgcccca gaccacctct ccgggccggt tgcggacacc
     3001 cggcgggcaa ttggcctcga gccactcacg ggtctcgagg cggaaagctt ccagatcgct
     3061 cattgcatcc cttttcccag acagcaaccg ctcattctag cctgtccaaa gggctcggtc
     3121 atgacgcaat cgcccgtccg gcaactcacg atacggcttc ccagtaacgt ctgagcgcat
     3181 cgatgtgatc ctgcggcgtc tcgagtccgg catccatgcc acgcatggag acgtagtccg
     3241 ccccggctgc ctcccaggcc tccaggtccg cgcgccagcg gtcgggcccc gcgccgtaat
     3301 tgaccatcgc ctcgatgccg aagccggcct gctccctgcc cgcctcggcg aggaagcccc
     3361 ggatcttccc gaggccggca agcccatcct tgatgctgcc gccgaagatg aacccgtcac
     3421 ccatccgtgc ggcgcggcgg taggcgacgt ccgagaaccc gcccagccag atgggtatcc
     3481 ggcggccggg cagcggcttc aggcccgcgc gctcgatgcg atgatagttg cccgagtaat
     3541 cgatcaccgg ctccgcccag aggcttcgca gcacctcgat ctgctcttcc tgacgcttgc
     3601 cccgattggc gaagttctcc ccgaggctgt cgtactccac gtagttccag ccggtgccca
     3661 cgccgagcct gagccgcccg ccggagagga tgtcgatctc cgccgcctgc ttggccacca
     3721 gcgccgtctg ccgctggggc aggatgatga ccccggtgac cagctcgacc cggcgggtca
     3781 ctgccgcaaa atatccgaag agcacgaacg gctcatggaa agccgactct tccgtgtaag
     3841 gcccccacag cgcgggctca cgcccggcgt gcaccgcgcc cagcacgtga tcgtaggcca
     3901 gaaggtggct gtagccgaga tcctcggcgg tctgggccca gtcgcgaagg accgccggat
     3961 cgctgccgat ctccagatgc ggaaagaccg cccctatctt catttcattc tccctgtatc
     4021 agacgagccg agccttcagc ggcctttgaa ctgaggtttg cgtttctcca cgaatgcccg
     4081 catggcttcc ttcgaatcct cggtcttagc cagctgtccg gtcatggtct gctcgaagcg
     4141 gtaaccgtcg cgaaggctca gctcctcgat ggtgttgagc gcgtgcttgg caagccggat
     4201 ggcgatgggg ctcttggccg cgacctcggc cgcgagggcg agcgcctcgt ccatgagcgc
     4261 atccggggcc acgcaggcct ccacgatgcc gagccggtaa agctcgggac cgtatacccg
     4321 atatccggtg agcatcatcc gccgcagccg ggagtgaccg aacagccgct gcgcgtggcg
     4381 gccgccgccg agcaggccca cgtcgatctc gggaagaccg agacaggcgg tctccgcggc
     4441 aatcagcatg tcgcaggaag cggcaaaggc caggccgccg ccaagggccg gcccgttgag
     4501 cgccccgatc acgggcttca cgcattcgcg cacgctgtgg taagcctccc gggccgcccg
     4561 gctgctctgc cagtgatcgc cgggttcagg tgcgcgtccg gagcgggact tgatgtccgc
     4621 gcccgcgcag aagaccttcc ccgcgcccgt gagcacaact acccgtgctt cgtcctggtc
     4681 gctgatggca tcgaacgtcc acgccatctc ggtgagcacc tgaccgctgt gagcattgac
     4741 cggcggatta tcgatggtca ccacggcgat gccgtccggc cgcaattccg tcttcaccaa
     4801 ttcgggtgtc atgaaccctc ccccaaggac tgctgacggg gtccattgta acgctctgga
     4861 tctgagccgc taccaaggcc cggcatcgtg gtttaagctt ttcccacggt ctggcggaac
     4921 caccgcggca atcggaggca gacatgagca agagccaacc caaccagctg ctgctgacca
     4981 acgcgcgact ctttcccgga gtgagcgacg aggtcttcgc cgacggagcc gtctggatcg
     5041 agggccgcac catccgctac gccggcccgg gctgcgatct gcccgccgtg gccgacgacg
     5101 tgccccgcgt cgacctccac gggcagttcg tcatgcccgg catgacggag tcccattctc
     5161 acctttccta ctccaacaac ggccccaccg agctggacaa gtctccggtg gaggacgcca
     5221 tgatccacgc cgtggcaaat gcccgtctga tgctcggctc gggcttcacg tcggccatca
     5281 gcttcggatc cgtgcaccga atcgacgtct ttctccgcga tgccatcgat gcgggcagaa
     5341 taccggggcc gcgcctggag gcgtcgagcc gggacgtggg ggcgacgtcg agcaatgccg
     5401 atcttcaccc ggactatttc aagccccaga tggagggcct cggcatgctg gccgatgggc
     5461 cctgggccgt gcgcaaggcc gtgcgaacca tccgcaagaa ccgcggcaac gtggtcaagc
     5521 tgttcctgga cggcgagggg cccggcgggc acgccccgcc cggggagctg acgtataccg
     5581 acgaggaagt cgcggcagcc gtcgacgagg cccaccggcg cggcatgcgg gtcgcctgcc
     5641 attcgaggtc tgccgcggcg gtgaagcagg ccgtgcggtt cgggatcgat ttcatcgggc
     5701 acgccaacta cctcgacgac gaggccctgg acatgctgaa acgaagcaga gagcgcctgt
     5761 tcgttggtcc cgccatcgcc tgggagctgg cctttctgcg ccacgccgcg gagctcggct
     5821 cgcccccggg gtcgcagagg tacaagttct acgagcagga ggtggaagcg accaagaacg
     5881 ccgtccgacg ccttcgcgaa gccggcgtgc gggtcctcat cggcggggac tacggcctga
     5941 gcatcacgcc tcacggcacc tacgccaagg acctccagta cttcgtcgag ctctttggcc
     6001 tgtcgccggc ggaggcgctg ctgtgcgcaa cccgggacgg cggcgcggcg gtcgatccct
     6061 ccggcatgct cggaacgctg gaggaaggca agctagccga cctggtgatc gtcgacggcg
     6121 accccctgga agacatcacc gtggtgcagg atcacgaccg catcacggcg gtcatgaagg
     6181 acggcgtcgt gcaccgccgc ctcgccgtgg acaatcctta ccaggcctca cccgacgcgc
     6241 tgcaggagga gctggcggca agagccgcag accccgagcg tcagcggcag gcggccgttc
     6301 tcctggagtc cagcggctga aagcaccagc gacacccggg gctggcatga ccggtcccgc
     6361 tggatgaacc tccagacact tgccacgccc cggatcggct ggcgctggcc ggcggccctc
     6421 attctgtttc tgctcggttt cgcgggattt gccacaggcg tcgcgccctc agagcggccc
     6481 gacgtggccc aggccgatct gttgaccaag gcctactaca gcctcgggct gttcgtggtg
     6541 ggcgggctgg acatcggcac gccggcgtcc ggcccgctgc tgggccgagc catgctctgg
     6601 atcgcctact tcggagcgcc tctgctgacg gcctcggccg tcatcgaggc cctggtccgc
     6661 gtgatggcgc cgcaccgttg gcagatgcga cgcctgcacg accacgtcgt catcgtgggc
     6721 tcgggggagc tcaccatcag ctacctgcgc gtgctgcggg ggagtcaccc gtcgacccca
     6781 gtcgtggtcg tggacgaaca cgtcgacccc gtgcgcgagc aggaactggc ccaggcgttc
     6841 gacgtgcgcg tcgtaatcgg caacatcact cacgacttcg tccagaacgg gctgcgcctg
     6901 gaccgcgcgg ctcgcgtcgt cctgctcggc gacagcgact tccagtcctt cgaggcggca
     6961 agcaagatgc tgcggcgctt cccccatctc gaatcacggc tcgttcttca ctgccagcgg
     7021 ctgcggttcc tgcgatcaat ggaggcaacc accgtcggcc gcctgtgcat cacgttcaac
     7081 tcctatcacc tggcggcggc cagcctcgtg aagcaacagc tcatcggcca tttcgagaag
     7141 accgaacacc gcgacgtcgt ggtgctggcc ggcttcggcc gcttcggcca gaccatccta
     7201 gaggagctgc aaaatctggc ccgggacgag ctggacaccg tggcgatcat cgacgtcgac
     7261 gcggaccggc gggtgatggt cgccgaagag cagcgtcggc tgggcggcaa ctaccgacgg
     7321 gtggtattgc agggggacat ctcccacccg gaggtctggc ggtccctggc cgcctccgtc
     7381 gatctctcca aggactcgcc gacggtgatc ctcggcaccg gccgggccga ggacaatctg
     7441 cgcacggcgc tgtggctcaa gcagagctat ccgaacgcgt tcgtgtttgc ccgaaccaac
     7501 gacaagtccg agctcgcggt cgaggtgggc agcgagcacg ggatcaggaa catcagcatc
     7561 cggcagctcg tggaggacaa cattcccgag gagtggcttg cctgactgat cgggtcagcc
     7621 gttgaggtgg cgggacagat ctccgacgtt ttccaggtac acctcgcgtc tgacgagcac
     7681 ccagccctgg atcatcagct cgtactgatc gatgtagcgc ccgccgacga tgatctcgtt
     7741 ggccccgccc agagactgat agacggtgaa gtagctgcgc acgtcggcgt tgcgaaccgc
     7801 gttgtcgaac tcgaagatgc tgttggtgac cacgtgcttg gttcgcggcg agcaggcgcc
     7861 gcgctgatac ggaacaacgt tctcctcgtc atcgtagaag atgatgacgt cggaaaacgt
     7921 ccgcagcacc tcttccggac cgttcagctc gctgccgtcc ggcatgacca tgacgccatg
     7981 ggcaaacaga ggcgccacgg tttctagatt tcccgtgtcg atgccctcgg cgtagccgta
     8041 catgatgtcc tcgatggcgt gccgcccctg agaaatcttg accgtgcgtt ccatctcacc
     8101 ctccgtgcca ggcgacatta tgcccattca cccgccatgg agacagccac gggccgcctc
     8161 ggcgctataa tgcagcgtct tccgggggga tccagatcga tgtgcagagc aaggatcgag
     8221 gcgccatggc caagcgactg atcgtgatgg ccgggctgct ggcgctggtc ttcggcagcc
     8281 tgttcggctg gaaggcgttc gagcgccaca aaacggccga gtatctggca aacgcccggc
     8341 cgccggcggt ggtcgtctcg tcaacgaggg tggaagagga ccgctggcgg cagcaggtgg
     8401 aatccgtcgg ctcgttgagc gccatccagg gcgtggacgt cagctccgag gtgcccggca
     8461 ccgtcgcgga catcagtttt caatccgggc agcagatcag cgcaggcgag ctgctcgtcc
     8521 agctcgacgc caccgccgaa cgggccgagc tgcgctcgct ggaagcccag ctggtgctgg
     8581 cgcgactgga ctacgagcgc gctttaggtc tccttgagcg cacgatgttg tcacaggcgc
     8641 agctcgatcg ggccaagagc gtgatggaca gcctgcaggc gcaggttgag gagcaggccg
     8701 ccttcattgc caagaaggcc atacgggcac ccttcgccgg cgagctcggt atccggcaag
     8761 tcaaccttgg cgagtacgtc tccccgggca cgcccatcgt cactctgcag gcgcttgatc
     8821 ccatctacgc gaacttcgcg ctcccggaac ggttcctgcg cgagctcgcc gtggaccagc
     8881 cgctgcgcgt ggtcgtggcc gcctacccgg aggatgagtt cctcggcacg gtgaccgcca
     8941 tcagccccaa ggtcgaggag accacgcgca acgtcgtggt ccaggctacc attgccaacg
     9001 ccgatcgcag gctccgtcca ggaatgttcg cgcgcatcac cgtgcagacc ggcggggacc
     9061 accgggtgct gacggttccg cgaacggcga tcaccttcaa tccctacggc gagtccgtct
     9121 tcctcatcaa cgagcaggac ggcgctctgc tggtcgaccg ccgccaggtc atcaccggag
     9181 agatccgcaa cgagcgcgtg gaggtcgttt ccggcctgct ggccgacgat ctcgtggtgg
     9241 ccaccgggca gctgaagctg agagccggac aacgcgtgaa gatcgacaac agcatcgatc
     9301 tttccaggcc ggtgacgggc ccttgaactt caccgatacg ttcgtccggc gtccggtgct
     9361 ggccagcgtg atcagcctgc tcgtgctgct ggtcggcatc cgggccgccg ggctgctcga
     9421 gctgcgggag tatccggaaa gcaaccgcgc cacgatcacg gtcaccaccg cctaccccgg
     9481 cgcggacgcg gacctggtgc agagcttcat caccgtgccc ctgcagcgcg ccatctcaga
     9541 agccgagggg atcgactacc tgacatccga ttcccgtcag ggggtttcga tcatcgaagc
     9601 cttcatggag ctcaactacg acccgaacgc agccatcgcc gagatccagg ccaaggtggc
     9661 cagccagcgc aacctcctgc cccaggaatc catggacccg gtgatcgatt cgcgcacgag
     9721 cctgggttgg gcgctcatgt acctcgcctt caccagccag gagatgtccc ccgctcaggt
     9781 gaccgactac ctgcaccgca gcgtcatccc gcggctgcag gcaatccccg gcgtcgccaa
     9841 ggcacagatc aacggaaacc cgacccttgc catgcgcatc tggctgaatc cgcaccgcat
     9901 ggcagccctg ggcgtcacgg cgaccgacgt gcgggcggcg ctgcaacgca acaacttcca
     9961 ggccggcgtc ggcgagacca agggcgacta cgttcgcgtc accctgagct ccaccaccgg
    10021 catcaccgcc agggaagatt tcgccaggct gatcatccgc agggacggcg agacgctgat
    10081 ccggctgcgc gacgtcgccg aggtgaatct ggattcgaag aactacgacc agaccagctg
    10141 gtacaacggc caaccggcca tcctcctggc cctggagacc agtccaggcg ccaatcctct
    10201 gaccgtttcc caggccgtcc gcgtcctgct gccggacatc gaaagccagc tgccatcgaa
    10261 catggagatg cagctcgccc acgacggcag ccagtacatc gacgactcca tcaacgaggt
    10321 gctgcgaacc ctgctggaag ccatgctcat cgtgctgctg gtgatcttcc tttccctcgg
    10381 ctcctggcat gcggccgtgg tacccgccgt ctccgtgccg ctctccctgg tcggcgcctg
    10441 cgcgctcatg ctggccttcg gcttctcgct gaacctgctc accctgctgg ccatgctgct
    10501 cgccatcggg ctggtggtgg acgacgccat cgtggtggtg gagaacgttc accgccacat
    10561 cgccatgaac aagacgccgt ttcaggcggc catagacggg gcccgggagc tgggactgcc
    10621 cattctctcc atgacgacca ccctgctcgc cgtctacgcc cccatcgcct tcatgggcgg
    10681 gctggtgggc atcctcttca cggagttcgc gttctccctg gccggagccg tgctcatctc
    10741 ggggctggtg gctttgacgt tctcgcccat gctctcctcc aaggtgctta ccagcggcga
    10801 ggagccgggg cgattcgaaa gcgccgtaga gcgtttcttc gcccgactcg ccgagcgcta
    10861 tcgggcgagg ctctcgagag ccctcgacta ccagcccgtc acgtacctct tcgccatcgt
    10921 cgtcatgggc agcatctact tcatgttcac caccagcaac accgagcttg cgcccaccga
    10981 agaccgggag gtgctgaacg ttcagatcac cgcgccggaa accgccacgc tggaatacgc
    11041 cgtggcacac gtccggcctg cagtgaagat gttcgagacg tttccagagc agcggcgaac
    11101 cttcctgatg atcggcggcg gcggcacgcc ggccaccagc ttcggcgggt ttcgcttcac
    11161 gcccgtcgcg gagcgcgagc ggtcccagat ggagatgcag cccatcctgc agaggatgct
    11221 caacgagatc gccggcgtgc agatcgccgt gttctcgttc ccctccctgc cgggcgctgc
    11281 cagaggtacg ccgctgcagt tcgtgatcgc gtcggatcag gactaccggc agctggacac
    11341 cctggccgac gacgtggtgc aggccgctta tcggagcggc aacttcctgt ttctcaacaa
    11401 atcggtggag ttcgcgctgc cccagaccca gatcgtgatc gatcgggacc gggcaggtga
    11461 tctcggtatc gagatgaacg acatcggcgt cgcgctcgcg acgctgctgg gcggcaacta
    11521 cgtcaaccgg ttcagcctcg caggccggag ctacgaggtg atcccccagg tcgagcgccg
    11581 cttccgcgcc agccacgaga tgctgaacga caactacctg cga
//
LOCUS       DBNHKV010000033         3643 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1917870, whole genome shotgun sequence.
ACCESSION   DBNHKV010000033 DBNHKV010000000
VERSION     DBNHKV010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1917870"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="AC16PP_00950"
     CDS             complement(<1..279)
                     /locus_tag="AC16PP_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR6480217.1"
                     /translation="MKAVIIEGERLSWQEAEEPQAGVGEVLIAVHATAVNRADLVQRS
                     GGYPPPPGASKILGLECAGEVLAIGEGVTRVKPGDRVCALLAGGGYAER"
     gene            complement(328..1107)
                     /gene="hisF"
                     /locus_tag="AC16PP_00955"
     CDS             complement(328..1107)
                     /gene="hisF"
                     /locus_tag="AC16PP_00955"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR6480218.1"
                     /translation="MLSKRLIACLDVRDGKLAKSVKFVDTKDIGDPVEKAREYYLDGL
                     DELVFYDITASSDKRRIMLDVVEAVAEQVFIPLSVGGGVSSVGDATDLRLAGAEKINV
                     NSAAVRRPQLIAECAEAIGRQNVVLSMDIRRVEPCAELPSGYEIVIEGGRRPMAIDAL
                     EWALEGERLGAGELVINSIDADGTRDGYELTLTAMLAEAVRIPVIASGGAGKPEHLYD
                     ALTVGKADAALVASMVHYGDYKVSELKRYLDGRGVKMRMSW"
     gene            complement(1101..1724)
                     /gene="hisH"
                     /locus_tag="AC16PP_00960"
     CDS             complement(1101..1724)
                     /gene="hisH"
                     /locus_tag="AC16PP_00960"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR6480219.1"
                     /translation="MSHAIIIDYDAGNLRSVQRACSEVGIDAEISADADRVAAADRLI
                     FPGVGAAGSAMRSVRRNGLDQALRSALAGGTPVLGICLGLQISFDHSEEDDQDTLGLL
                     PGLVKRFAFERRDLKIPHMGWNEVRVVRPHPLLDDIEPGDEFYFVHSYYPVPAEPELV
                     FAVTDYERDFACAVGRDNYFATQFHPEKSGRVGLRLLARFAAWDGRC"
     gene            1817..2827
                     /gene="hemH"
                     /locus_tag="AC16PP_00965"
     CDS             1817..2827
                     /gene="hemH"
                     /locus_tag="AC16PP_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR6480220.1"
                     /translation="MTRGLLLVNLGTPLSSDVRDVRAFLGEFLMDRYVLDVPWLLRRL
                     IVTGFILPFRPNQTARAYAKIWNAAGPETGSPLLHYSELCRHGVEERTGMPTALAMRY
                     GTPSVRDAIAALLGHGVRQITMLPLYPQHADSTITTSIEAVRRSLPTNVSLTVVPPFY
                     SQPDYLDAQAAAIRENLPASWDHLLFSYHGLPERHITKADPTGNHCLALENCCQIPSE
                     AHATCYRHQVYRTSELLAQRLGLSEDQYSVSFQSRLGRLPWLTPYTDQVLEALPGKGV
                     RRLAVTCPAFVADNLETLEEIGIAGRESFLAAGGESFSLVPCLNDRADWIDAIVRIAG
                     AA"
     gene            complement(2881..>3643)
                     /locus_tag="AC16PP_00970"
     CDS             complement(2881..>3643)
                     /locus_tag="AC16PP_00970"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6480221.1"
                     /translation="SKRIAALERYYDRDLIERHGRRVVLTHHGTRLVEKVTPVISELR
                     SVFLEDNALRKGKIIIGVSEAILSSWGPRLFARVRSELPEVELEFHAQRSPVVLDRIR
                     SGEYMVGICTGSPDADTDLQSEVIRLEPMVIIPSALAPLDYEVGDELDVITIESRSGA
                     WRSIEDDMRRQNLRRVVPLESFFGVAQMALAGFGHGLVPEGVAQTLGVTAGERIPLDS
                     AGLNRPVRFVARKSMFSQQLVQSFYQAVSRFAAEV"
BASE COUNT          637 a         1298 c         1095 g          613 t
ORIGIN      
        1 gcgctccgcg tagccgcccc cggccaggag cgcgcagacc cgatcgccgg gctttacccg
       61 ggtcacgcct tccccaatgg ccagcacctc tcccgcgcat tccaggccca ggatcttgct
      121 cgctcccggt gggggcgggt agcctcccga gcgctgcacc aggtccgcac gattgacggc
      181 cgtcgcgtgc acggcgatca gcacctcacc gaccccggcc tgaggctctt cggcctcctg
      241 ccagctcaga cgctctcctt caatgatgac ggctttcatt gctctccctg actggtaatg
      301 gcctttttcg ctcgcccacg ctgatgctca ccaggacatc cgcatcttga ccccgcggcc
      361 gtcgaggtac cgcttcagct cgctcacctt gtaatcgccg tagtgcacca tggaagccac
      421 cagcgcagcg tccgctttcc ccacggtcag cgcatcgtaa aggtgctcgg gcttgcccgc
      481 accacccgag gcgataacag gaattcgcac ggcctccgcc agcatggcag tgagggtgag
      541 ctcgtagccg tcccgggtcc cgtcggcatc gatggagttg atcaccagct cgcccgcgcc
      601 gagccgctcc ccttccaggg cccactccag agcgtctatg gccatgggcc gacggccgcc
      661 ctctatcacg atctcgtaac cggaaggcag ctccgcgcag ggttccacac gtcggatgtc
      721 catggacaag accacgttct gacgcccgat ggcctccgcg cactcggcga tgagctgcgg
      781 gcgccgcacg gccgccgagt tgacgttgat tttttccgcc ccggccaggc gcagatccgt
      841 ggcatccccc accgagctga ccccgccgcc aacagagagc ggaatgaaaa cctgctcggc
      901 cacggcctcg acgacgtcga gcatgatgcg acgcttgtcg ctcgatgcgg tgatgtcgta
      961 gaagaccagc tcgtcgagcc cgtccaggta gtactcgcgg gccttttcga ccggatcccc
     1021 gatgtctttc gtgtcgacga acttcacgct ctttgcgagc ttgccgtcgc ggacgtcgag
     1081 acaggctatc agacgcttgc tcagcatcgg ccgtcccagg cagcaaagcg cgcgagcagc
     1141 cggagcccga cccggccgct tttctccgga tgaaactggg tggcgaagta gttgtctctg
     1201 cccaccgcgc aggcaaagtc gcgctcgtag tcggtcaccg caaagacgag ctccggttcc
     1261 gcgggcaccg ggtaatagga atgcacgaag tagaactcgt cgccgggctc gatgtcgtcg
     1321 agcagcgggt gcgggcgcac cacccggacc tcgttccagc ccatgtgcgg gatcttcaga
     1381 tcacgccgct cgaacgcgaa gcgtttgacc agcccgggaa ggaggcccag cgtgtcctga
     1441 tcgtcttcct cggagtggtc gaaggatatc tgcagcccca ggcagatgcc gagaacgggg
     1501 gtcccgcccg ccagggccga gcgcaacgcc tgatccagcc cgttgcggcg cacgcttcgc
     1561 atcgcgctcc ccgccgcgcc gacgcccggg aagatcagcc ggtcggcggc cgccacgcgg
     1621 tcggcatcgg cgctgatctc cgcatcgatg ccgacctccg agcaggcccg ctgaacactg
     1681 cgcaggttgc cggcgtcata gtcgatgatg atggcgtggg acatcccgtg ggatccggta
     1741 ggaaaagtga catccaaaac gagcgcggat actatcccat cgcgcttggg caaactacat
     1801 tcaccggaga cctcccgtga cccgaggcct gctgctggtc aacctcggca ccccgctttc
     1861 gagtgacgtt cgcgacgtgc gcgcgttcct gggcgaattt ctgatggacc gatacgtcct
     1921 cgacgtgccc tggctactgc ggcgcctgat cgtaaccggg ttcattctgc cgttccgtcc
     1981 caaccagacc gcccgggcct acgccaagat ctggaatgcg gcaggaccgg aaaccggctc
     2041 gccccttctg cattactcgg agctatgccg tcacggggtc gaggaacgca cggggatgcc
     2101 tactgctctc gccatgcgct acgggacccc cagcgtgcgg gacgccatcg ccgccctgct
     2161 cggacatggc gtgcggcaga tcaccatgct tcccctgtac ccgcagcacg ccgactccac
     2221 catcaccacc agcatcgagg ccgtacgacg aagtctgcca accaacgtta gcctgaccgt
     2281 ggtgccgcct ttctactcgc agccggacta tctggatgcc caggccgccg cgatccgaga
     2341 gaacctgccg gcgtcctggg atcacctgct cttcagttac cacggtctgc ccgagcggca
     2401 catcaccaag gcagatccca ccggcaacca ttgccttgcc ctggagaact gctgccagat
     2461 cccctccgag gcgcacgcca cctgctaccg gcatcaggtc taccgcacct ccgagctcct
     2521 tgcccaacgc ctgggcctct ccgaggatca gtacagcgtg agctttcagt cgcggctggg
     2581 acggctgccg tggctcaccc cctataccga ccaggtgctg gaagccctgc caggaaaagg
     2641 ggtcaggcgt ctcgccgtca cctgcccggc cttcgtcgcg gataatctgg aaactctgga
     2701 ggaaataggc atcgccgggc gcgagagctt cctggctgcc ggcggagagt cgttctcgct
     2761 ggttccctgc ctgaacgacc gcgcggactg gatcgacgcc atcgtccgaa ttgccggggc
     2821 ggcctagagc ccgcctccgg ccacatcggt catcaccgga aaaacgggtc ttcgaagcgg
     2881 tcacacctcg gcggcgaaac gggaaaccgc ctggtagaaa ctctgtacca gctgctgcga
     2941 gaacatggat ttccgggcaa cgaaacgaac gggccggttc agccctgcgc tgtccagcgg
     3001 tatccgttcg ccggccgtca cccccagcgt ctgggcaacc ccctccggca ccagcccgtg
     3061 accaaaaccg gccagggcca tctgcgccac gccgaaaaat gactccagcg gcacgacgcg
     3121 cctgaggttc tgccggcgca tgtcgtcctc gatggagcgc caggcacccg agcgcgactc
     3181 gatggtgatc acgtccagct cgtcacccac ctcgtaatcg agaggcgcca gcgcagacgg
     3241 gatgatgacc atgggctcga gcctgatgac ctcgctctgc aggtccgtat ccgcgtccgg
     3301 cgagcccgtg cagatgccga ccatgtactc accggagcgg atccggtcga gcaccaccgg
     3361 cgaacgttgg gcgtgaaact cgagctccac ctccggcagc tcggagcgca cccgggcaaa
     3421 cagtcgaggg ccccaactgg agaggatggc ctcggaaacg ccgatgatga tcttgccctt
     3481 gcgcagcgcg ttgtcctcca ggaacacgct gcgcagctcg gagatcaccg gggtgacctt
     3541 ctccaccagc cgcgttccgt ggtgggtgag caccacccgc cggccatggc gctcgatcag
     3601 atcgcggtcg tagtagcgct cgagcgcggc gatgcgtttg ctc
//
LOCUS       DBNHKV010000034         1785 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1493495, whole genome shotgun sequence.
ACCESSION   DBNHKV010000034 DBNHKV010000000
VERSION     DBNHKV010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1493495"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="AC16PP_00975"
     CDS             complement(<1..557)
                     /locus_tag="AC16PP_00975"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6480222.1"
                     /translation="MKLLVTGLSGLIGGAAHPVLAADHDVAALNRREVNGTFTWQADI
                     ADYEAIRPAFDGVDAVIHLAAKPGEHFGWDALRVTNVEGTRNVFQAAVEAGVPRVVFA
                     SSGATVAGWEHDEPYRALVEGRYDDLPDRWPMITVDAPPRPRGVYGSTKVWGEALARH
                     FADTTATSFISVRIGFVNAEDRPANA"
     gene            complement(554..1513)
                     /locus_tag="AC16PP_00980"
     CDS             complement(554..1513)
                     /locus_tag="AC16PP_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6480223.1"
                     /translation="MDEIVPRHIRDPRIETTFVDANGLRFEVDACGSGRRLALCLHGF
                     PEHSFSWRHQLPMLADLGFRAWAPNLRGYGLTSRPFGVDAYAIENLMDDVAGLIDASG
                     CRETVLIAHDWGALVAWYFAMRKVRPLEKLIICNVPHPGAGRASGRSFAQLRKSWYVF
                     FFQLPRLPENLLGRRGARAVADAFRGVAVDPSRFPDEVVDVYRRNAAQPGALEAMINY
                     YRAALRGGGFRRQSALGFPVIETPTLMVWGEEDAALGIETTRGTDDYVANLTLRYLPR
                     VSHWVQQEQPEVVNAMMRAFLENEPVPYLTWEPKLVQAVKEEA"
     gene            complement(1506..>1785)
                     /locus_tag="AC16PP_00985"
     CDS             complement(1506..>1785)
                     /locus_tag="AC16PP_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480224.1"
                     /translation="GIVDYFIDLTAEFIRRQRALGRSGAADPDRLARALILMSNAAAN
                     DNMTRHNPDGPAVIGAVLGDIWNSAIYGPGGDGTSGCPVELKGGSIRG"
BASE COUNT          317 a          608 c          586 g          274 t
ORIGIN      
        1 gcgttcgccg gccggtcctc ggcgttgacg aaaccgatgc ggaccgagat gaaggacgtg
       61 gcggtggtat cggcaaagtg gcgggcaagc gcctcgcccc agaccttggt gctgccgtaa
      121 acgccgcggg gtcgcggggg cgcgtccacc gtgatcatcg gccagcgatc gggcaggtcg
      181 tcatagcgcc cctcgacgag cgcgcggtaa ggctcgtcgt gctcccaacc ggccaccgtg
      241 gccccgctgg aggcgaaaac gacccgcggc acgccggcct ccacggcggc ctgaaagacg
      301 ttgcgcgttc cctccacgtt ggtgacgcgc aaagcgtccc agccgaagtg ctcaccgggc
      361 ttcgccgcca ggtgaatgac ggcgtcgacc ccgtcgaacg ctggccgaat ggcttcgtag
      421 tcggcaatgt cggcctgcca ggtgaacgtg ccgttcacct cccggcggtt cagcgccgcg
      481 acgtcgtggt cggctgcaag aaccgggtgg gcagcgccac cgatgagccc gctcaagccc
      541 gtgacgagaa gcttcacgct tcctccttta cggcctgcac gagcttcggc tcccaggtga
      601 ggtagggaac cggctcgttc tcgagaaaag ctcgcatcat ggcattgacc acctcgggct
      661 gctcctgctg aacccagtgc gagacccgcg gcaggtagcg cagggtgagg ttcgcgacgt
      721 agtcgtcggt gccacgcgtg gtctcgatgc cgagcgcagc gtcctcctcg ccccagacca
      781 tgagggtggg ggtctcgatg acgggaaagc cgagcgcgct ctggcggcga aaaccgccgc
      841 cgcgcaaggc cgcccggtaa tagttgatca tggcttccag cgcgccgggc tgcgccgcgt
      901 tgcgccggta aacgtccacc acctcgtccg ggaagcggct cggatccacg gcgacccccc
      961 gaaacgcatc cgccacggcc cgtgcgcctc ggcgacccag aagattctcg ggaaggcggg
     1021 gaagctgaaa gaagaacacg taccaggact tccggagctg agcgaacgat cgccccgatg
     1081 ctctgcctgc cccgggatgc ggaacgttac agatgatcag cttttccagg ggacggacct
     1141 tgcgcatggc gaagtaccag gccaccaggg ctccccagtc gtgggcgatg agcaccgtct
     1201 cccggcaacc ggacgcatcg atcagccctg ccacgtcgtc catgaggttc tcgatggcgt
     1261 aagcgtccac cccgaagggc cgcgacgtca ggccgtaacc cctcaagttc ggcgcccagg
     1321 cccgaaagcc gagatctgcc agcatgggca gctgatgacg ccaggagaag ctgtgctccg
     1381 gaaagccgtg cagacacagc gcgaggcgcc ggccggagcc acaggcgtcc acctcgaacc
     1441 tgaggccgtt ggcatcgacg aacgtcgttt cgatgcgggg atcccgtatg tgccgaggaa
     1501 cgatctcatc cacgaatgct gcctcccttc aactcaaccg ggcacccgct ggtgccatcg
     1561 cctcccgggc cgtagatggc cgagttccag atatcgccga gaacggcgcc gatcaccgcc
     1621 ggcccatcgg gattgtggcg ggtcatgttg tcgtttgccg cggcgttgct catcagaatc
     1681 agcgcccggg ccagtcggtc cggatcggcg gccccgcttc gacccagcgc ccgctgccgg
     1741 cgaatgaact ccgccgtgag atcgatgaag taatcgacga tcccc
//
LOCUS       DBNHKV010000035         6622 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1422742, whole genome shotgun sequence.
ACCESSION   DBNHKV010000035 DBNHKV010000000
VERSION     DBNHKV010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1422742"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="AC16PP_00990"
     CDS             complement(<1..750)
                     /locus_tag="AC16PP_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6480225.1"
                     /translation="MMAATLTQRIVESFERNAERPCLRVPGGRDWSYGELNRLAEKLA
                     GLLAGVPVAGGDRVLVQVEKSPEAVALYLACLRLGAVYVPINTAYTGSEVEYFLRDAE
                     PSLFVCRPSVQPALAEIAGSVPVLTLGADGDGSLLEGALKAEARDEAEPRDPADLAAI
                     LYTSGTTGRSKGAMLSQGNLLSNAESLLECWVWRDDDVLLHALPIFHVHGLFIALHCA
                     FLSGTPVTFLAKFDPEAIVECLPESTVLMGVP"
     gene            737..1900
                     /locus_tag="AC16PP_00995"
     CDS             737..1900
                     /locus_tag="AC16PP_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6480226.1"
                     /translation="MAAIIIAVLKTNDCGSPRFGCRVRTGNGGNLAGSINQTALLRNL
                     SLITLLLVLAGCDGQETTARPGPSGAWGGPTTVVTVAAVKRPRRTRVEALGTAVANES
                     VTITAQVTDSVSQVRFEDGDYVNAGDVLVELTNEEETALLAEAEANARDTRTQLDRLE
                     DLVLQGSVPISQVDEARARHSAAVARYQSVVARLEDRLITAPFSGVLGFRQVSTGTLI
                     TPGTPITTLDDVSVIKLDFSIPEIHLSRLQPGQRIEAESPAYSGSVFDATVSTIGSRV
                     DPVTRAAVVRAHIDNGDMRLRPGMLLTARLTTSEREVLMIPEAALLQRGTQVRVFTEV
                     DGTARSRLITHGVRDAGWVEVREGLTEGERVITDGLIKVRDGSPITTVRAEQG"
     gene            1906..4998
                     /locus_tag="AC16PP_01000"
     CDS             1906..4998
                     /locus_tag="AC16PP_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6480227.1"
                     /translation="MWLSDISVQRPVFASVISLALIAFGALSFNLLPLREYPDINPPL
                     VSISTAYVGASAEVIESRVTQVIEDQISGIEGIKSISSSSRDEASNINIEFELNRNID
                     EAANDVRDRVARIVDRLPDDVELPIVAKQDSDARPVMYISLTSSRMSVMELNDYAERY
                     IVDRFEIIPGVASAAMHGSGKPSMRIWLDRLALAARDLTVIDIEDALRRENLELPAGR
                     IESAEREFRVRLARGYQSDEDFRQLVLGVGGDGHLIRLGEVADVNLAPSNTRESFRAN
                     RQNTVGLAITKQSTANTLATLEAVKAEIARVNEALPDHMEFVPSSDDSLFIREAINAV
                     YQTIAITTVLVSLVIFVFLGSIRSMIIPAVTIPVCLTSAFIALALFGYSVNLVTILAL
                     VLCIGLVVDDAIVVLENIHRRVERGEPPLVAAYRGSRQVAFAVIATTVVLVAVFTPIM
                     FLRDNMGVIFSELAVTVSAAVVFSSVLALSLTPMMSSKLLRKTTAKNPVTRMVERAFA
                     RLEAGYQRLLKRLLPYSWAAAVITLLVIGLAYWLMGMVPREYAPREDQGFFMAQVLAP
                     EGTGFDRMQEHMGDVEAPLMQMQDEGLISRVLVRVPSWGGTSPNTGMVIVSLEPWRER
                     DATTREIIDRASTAWEEIPAVRIFAFERSGLSRGGGGRPVQFVIGGPNYETLAQWRDL
                     LIARAMEYPGMTRVDSDLKETQPQLIVRVDKNRAAALGISVQNVGRTLAAMMSERRVT
                     TFVQDGEEYDVILQAKEDQRATAADLQNIYVRSESTGRLVPLANLIEVEETAGAGRLN
                     RYNRLRAVTIEAGLAPGYALADALEFLETVVREELPESAQVDYRGESLEFKEASGSLL
                     FTFAIALLIVYLVLAAQFESFVHPLVILLTVPMAIAGALLGLKLTGGTLNIYSQIGIV
                     MLIGIAAKNGVLIVEFINQLRDAGRSFDDAIVEAAGIRLRPVIMTTISTVMGSTPLIL
                     ATGAGSTSRSMLGVVIFSGVSLATLLTLFIVPAFYHLLARRTGSPEETARRLKGLIQS
                     "
     gene            complement(4989..5609)
                     /locus_tag="AC16PP_01005"
     CDS             complement(4989..5609)
                     /locus_tag="AC16PP_01005"
                     /inference="COORDINATES: protein motif:HMM:NF019339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6480228.1"
                     /translation="MMVERAPPIMLTLLLSSTLAAASETVLFNESAAFECYQAALHDG
                     GEYDIESCSRALEYQALTTIDRAATHSNRGILYARIGDIRNALRDHNRAVKLAPEVGS
                     IYINRSNTLVRAKRYEGAMRDLEKAIEIADDSLAYAHYNRALLFQKLGDLQAARADAE
                     QAAEIAPDSPAYRRFLAAFGETEGDPLLPDAEPGNPPEEPGGEGQD"
     gene            complement(5599..6234)
                     /locus_tag="AC16PP_01010"
     CDS             complement(5599..6234)
                     /locus_tag="AC16PP_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480229.1"
                     /translation="MKIGPVLICAFALAMAPFAGAQSVSAWDLVYPSNFNWEIRTHRV
                     RVANDLASRVSLLVAVVPRQTEEESARLRETEQTLEARGADASPRDRSRLYLSRAYQH
                     RRLLELLADTLDALQCVRSAEELPREVHCWTRAAVNLMDEETVDVALTVLRDARMVPR
                     DEDMPVKAQDPKVWYGEYGKGIVRHIVMPYLAGLAAPRTPAATEEEVSHDG"
     gene            complement(6231..>6622)
                     /locus_tag="AC16PP_01015"
     CDS             complement(6231..>6622)
                     /locus_tag="AC16PP_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate isomerase"
                     /protein_id="HIR6480230.1"
                     /translation="ANIDEELMLDALMLRLRDSAVATGVFPLPGIRVRAARRDRFVVA
                     DGDPENAFVHLMVRIGHGRTPEVRRQVADRLFAVLCDHLREAATRRGLGLSLEIQEID
                     PVGSLKRNNLHERLAGRAAGHVSVKVE"
BASE COUNT         1157 a         2223 c         2102 g         1140 t
ORIGIN      
        1 cgggaccccc atgagcaccg tggactccgg caggcactcg acgatggctt ccggatcgaa
       61 cttcgcaagg aaggttaccg gcgtgccgga gagaaacgcg cagtggaggg caatgaacag
      121 accgtgcacg tgaaagatgg gcagcgcgtg gagcagcacg tcgtcgtcgc gccagaccca
      181 gcactcgagc aggctctcgg cgttggacag caggttgccc tggctgagca tcgcaccttt
      241 ggagcggccg gtggtgccag aggtgtagag aatggccgcc aggtctgcag gatcacgcgg
      301 ctccgcctcg tcacgcgctt cagctttcag cgcgccctcg agcagcgacc cgtctccatc
      361 ggctccgagc gtcagaaccg gcacgctgcc ggcaatttcg gccagcgccg gctggaccga
      421 gggccggcag acgaagagcg agggctccgc atcgcgcaga aagtattcca cttccgagcc
      481 ggtgtaggcg gtgttgatgg gcacgtagac ggcgccgagg cgcaggcagg cgaggtagag
      541 ggcgaccgcc tcaggggact tttccacctg gaccagaacc cggtctcccc ccgccaccgg
      601 cacgccggca agcaggccgg ccagcttctc cgccagtcgg ttgagctcac catacgacca
      661 gtcgcgcccg ccgggaacac gcaggcaggg acgttcggca tttcgctcga acgactccac
      721 gatccgctga gtgagggtgg ccgccatcat catcgccgtc ctgaaaacga acgattgtgg
      781 cagcccccgc tttggatgtc gcgttcgtac cggcaatggc ggtaaccttg ccggctcgat
      841 caaccagacg gcgcttttga gaaacctctc cctcatcact ttgctcctcg ttcttgcagg
      901 atgcgacggg caggaaacca cggcccgtcc aggcccttcc ggggcctggg gcggcccgac
      961 caccgtggtc acggtcgcgg ccgtgaaacg gccccggcga acccgggtag aggcgctcgg
     1021 cacggcggtg gcaaacgaat cggtcacgat aaccgcccag gtcacggaca gcgtcagcca
     1081 ggtgcgtttc gaggacggcg actacgtcaa cgcgggcgac gtgctggtgg agctcaccaa
     1141 cgaggaggaa acggctctgc tggcagaagc agaggccaac gccagggata cccgaaccca
     1201 gctggaccgg ctggaagacc tggtccttca gggcagcgtg ccgatctctc aggtggacga
     1261 ggctcgcgcg cgccactcgg ccgccgtcgc gcgctaccag tcggtggtcg cccgtctcga
     1321 ggaccggctg atcaccgcgc ccttctccgg tgttctggga tttcgccagg taagcaccgg
     1381 cacgctgatc acccccggaa ccccgatcac gaccctggac gacgtctccg tcatcaagct
     1441 cgacttctcc atcccggaga ttcacctgag ccgcctccaa cccggccagc gaatagaggc
     1501 ggaaagcccc gcctattccg ggtcggtgtt cgacgcgacg gtaagcacca tcggctcccg
     1561 ggtcgatccc gtcacgcgcg ctgccgtggt gcgggcgcat atcgacaacg gggacatgcg
     1621 cctgcgcccg ggcatgctgc tcacggccag gctgaccacc tccgagcggg aggtgctcat
     1681 gattcccgag gcagcgctgc ttcagcgcgg tacgcaagtg cgcgtcttca ccgaggtcga
     1741 cggcaccgcc cgcagcaggc tgatcactca cggcgtacgc gacgccggct gggtggaggt
     1801 tcgggagggc ttgacggaag gtgagcgcgt catcaccgac ggcctcatca aggtgcgcga
     1861 tggctctccc atcacgacgg ttcgcgccga acagggctga gcatcatgtg gctttctgac
     1921 atctccgttc agaggccggt gttcgccagc gtgatcagcc tggccctgat cgcgttcggc
     1981 gctctgtcct tcaacctgct ccctctgcgc gagtatcccg acatcaaccc cccgctggtt
     2041 tccatcagca ccgcttacgt aggcgcgtcg gccgaggtca tcgaatcccg cgtcactcag
     2101 gtgattgagg atcagatcag cggcatcgag ggcataaaga gcatcagctc gtcgagccgt
     2161 gacgaggcct ccaacatcaa catcgagttc gagctcaacc gcaacataga cgaggcggcc
     2221 aacgacgtcc gggacagggt ggcccgcatc gtcgatcggc tgcctgacga cgtcgagctg
     2281 cccatcgtcg ccaaacagga ctcggacgcc cgtcccgtca tgtatatcag cctgaccagc
     2341 tcgcgaatga gcgtgatgga gctcaacgac tacgcggagc gctacatcgt cgaccgcttc
     2401 gaaatcattc ccggcgtcgc cagcgcggcc atgcacggca gcggcaaacc gagcatgcgg
     2461 atatggctgg accggcttgc gctggccgcc cgcgacctga ccgtgatcga tatcgaagat
     2521 gctctgcggc gagagaacct cgagcttccc gccggacgca tcgagtccgc agagcgcgag
     2581 ttccgagtgc gcctggcccg gggttaccaa agcgacgagg attttcgaca gctggtgctg
     2641 ggcgtgggtg gagacgggca tctgattcgc ctgggtgagg tcgccgacgt gaacctcgcg
     2701 cccagcaaca ccagagagtc gttccgggcc aatcgccaga acaccgttgg cctggccatc
     2761 accaagcaat ccacagccaa cacgctcgcc acgctggaag ccgtcaaagc cgagatcgcg
     2821 cgcgtgaacg aggcgctgcc tgatcacatg gagttcgtgc ctagctcgga cgattcgctg
     2881 ttcattcgcg aggcgatcaa cgccgtgtac cagaccatcg ccatcaccac ggtgctggtc
     2941 agcctcgtga tcttcgtctt tctcggcagc atccgctcga tgatcattcc cgccgtgacc
     3001 atccccgtgt gtctgacgtc ggcattcatc gccctggccc tgttcggtta ctcggtcaac
     3061 ctggtcacca tcctggccct ggtgctgtgc atcgggctgg tcgtggacga tgccatcgtc
     3121 gtgctggaga acatccatcg ccgggtggag cgtggcgaac ctccgctcgt cgccgcgtac
     3181 cggggttcga ggcaggtggc ctttgcggtg atcgcgacca ccgtcgtgct ggtggccgtg
     3241 ttcacgccca tcatgttcct gcgcgacaac atgggcgtga tattcagcga gctggctgtc
     3301 acggtttccg ccgccgtggt cttctccagc gtcctggccc tgtcgctgac gcccatgatg
     3361 agctccaagc ttctacgcaa aacgacggca aagaacccgg taacccggat ggtggagcgc
     3421 gccttcgccc ggctggaggc aggctatcag cgcctgctca aacgtcttct cccctacagc
     3481 tgggcggccg ccgtcatcac cttgctcgtc atcggcctcg cctattggct catgggcatg
     3541 gtgcccaggg aatacgctcc ccgggaggac cagggcttct tcatggctca ggtgctggcg
     3601 ccggagggaa ccggtttcga tcgcatgcag gaacacatgg gcgacgtcga ggcgcccttg
     3661 atgcagatgc aggacgaggg cctgatctcg agagtgctgg tccgggtccc cagctggggc
     3721 ggaacgagcc cgaacaccgg catggtgatc gtctccctgg agccgtggcg ggaacgggac
     3781 gccaccacac gcgagatcat cgaccgggcc tccacagcgt gggaggaaat tcccgccgtg
     3841 cggatcttcg ccttcgagcg ctccggtctg tcccgaggcg gcggtggccg cccggttcag
     3901 ttcgtcatcg gcgggcccaa ctacgagacc ctggcccaat ggcgcgatct tctaatcgca
     3961 cgcgccatgg agtaccccgg catgacgcgc gtcgacagcg acctgaagga aacccagccg
     4021 cagctgatcg tgcgggtcga caagaatcgc gcggcggcgc tgggcatctc cgtgcagaac
     4081 gtaggcagaa cccttgcggc catgatgagc gaacgcaggg tcacgacctt cgtgcaggac
     4141 ggcgaagagt acgacgtgat cctgcaggcc aaggaggatc agcgggcgac ggccgcagat
     4201 cttcagaaca tctacgttcg ctcggaaagc accggccgtc tggttccgct ggccaacctc
     4261 atcgaggtgg aggagaccgc cggagctggg cgcctcaatc gctacaaccg gctccgcgcc
     4321 gtgaccatcg aggccggttt ggccccaggc tacgccctgg cggacgctct ggagtttctg
     4381 gagaccgtag tgcgtgagga gctgccggaa tccgcccagg tggactaccg gggcgaatcg
     4441 ctggagttca aggaagccag cggatcactt ctgttcacgt tcgccatcgc gctgctcatc
     4501 gtctatctgg tgctggccgc ccagttcgag agcttcgtcc acccgctggt catcctgctg
     4561 accgtcccca tggcgattgc cggcgcgctg ctcggcctca agctgaccgg cggcacgctc
     4621 aacatctaca gccagatcgg catcgtcatg ctcatcggca ttgccgccaa gaacggcgtt
     4681 cttatcgtcg agttcatcaa tcagctgcgc gatgcaggcc gttctttcga cgacgccatc
     4741 gtggaagcag cgggcattcg cctgcgcccc gtcatcatga cgaccatctc cacggtcatg
     4801 ggatcgacgc cgctgatcct cgcgaccggt gccggctcga cgagccgcag catgctcggg
     4861 gtcgtcattt tctccggcgt ctccctggca acgctgctga cactcttcat cgttccggct
     4921 ttctatcatc tgctcgcccg gcgaacgggc tcgcccgagg aaaccgcgcg ccggctcaag
     4981 ggcctgattc agtcctgacc ctcaccgccc ggttcctccg gaggattgcc tggctcggca
     5041 tccgggagga gcgggtcgcc ctcggtttcg ccgaaggcag caaggaagcg ccggtacgcc
     5101 ggcgagtccg gcgctatctc cgcggcttgc tcggcatccg ccctcgccgc ctgaagatca
     5161 ccgagcttct gaaagagcag cgcccggttg taatgggcgt aagccagact gtcgtcggca
     5221 atctcgatcg ccttctcgag gtcgcgcatg gcgccctcgt agcgcttcgc ccggaccagg
     5281 gtgttggaac ggttgatgta gatgctgccc acctccgggg cgagcttcac cgcgcggttg
     5341 tggtcccgaa gagcgttgcg gatgtcgccg atgcgggcgt agagaatgcc gcggttggaa
     5401 tgggtggccg ccctgtcgat ggtggtcagc gcctgatact ccagagcccg gctgcacgac
     5461 tcgatgtcat actcgccgcc gtcgtgcagg gccgcctggt agcactcgaa cgcggccgac
     5521 tcgttgaaga gcaccgtctc cgaggcagcc gccagcgtcg atgagagcag caaagtcaac
     5581 atgatcgggg gggcacgctc aaccatcatg gctgacttcc tcttccgtgg ccgccggcgt
     5641 tcgaggcgcc gcgagaccgg ccagataggg catcacgatg tgccgcacga tgcccttgcc
     5701 gtactcgccg taccagacct tcgggtcctg agccttcacc ggcatgtcct cgtcccgagg
     5761 caccatgcgg gcatctctga gcaccgtgag cgccacgtcg acggtctcct cgtccatgag
     5821 attgacggcc gcccgggtcc agcagtgaac ctccctgggc aactcttctg ccgatcgaac
     5881 gcactgcagc gcatccagcg tatctgcaag caactccagc agccgccgat gctggtaggc
     5941 gcgcgagagg tagagcctgc tccgatccct tggggacgca tcggctccgc gcgcctccag
     6001 cgtctgctcg gtttcgcgca atcgcgccga ctcttcttcc gtctgccgtg gcaccaccgc
     6061 cacgagcagc gacacgcggg aggccagatc gttggccacc cgcacccggt gagtacggat
     6121 ctcccagttg aaattgctgg ggtaaaccag atcccaggcg ctgacgcttt gcgcgccggc
     6181 gaagggggcc atcgccagcg cgaaggcgca gatcagaacc ggcccgatct tcattcgacc
     6241 ttgacggaga cgtggcctgc ggcgcgcccg gccagccgtt cgtgcaggtt gttccgcttc
     6301 aggcttccca ccgggtcaat ctcctggatc tccagggaca gccccagccc gcgccgggta
     6361 gccgcctctc tgaggtgatc acacaagacc gcgaacagac gatccgcgac ctgccggcga
     6421 acctccggcg tccggccatg accgatcctg accatgaggt gcacgaaggc attttccggg
     6481 tccccgtcgg caacgacgaa gcggtcccgt cgggctgcgc gcacgcggat gccgggcagg
     6541 gggaacacgc ccgtagcgac ggcgctgtca cgcagccgga gcatgagcgc gtcaagcatc
     6601 agctcttcgt cgatgttcgc cg
//
LOCUS       DBNHKV010000036         3697 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_147357, whole genome shotgun sequence.
ACCESSION   DBNHKV010000036 DBNHKV010000000
VERSION     DBNHKV010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_147357"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="AC16PP_01020"
     CDS             complement(<1..264)
                     /locus_tag="AC16PP_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480231.1"
                     /translation="MPIVAIATLAILLAASSGCSMIHQDVGKKPPREASRALADRADA
                     TVDDVLEALGPPHAMSAINDGYAFLYHWYAVEERQLGLSSNWPV"
     gene            complement(264..632)
                     /locus_tag="AC16PP_01025"
     CDS             complement(264..632)
                     /locus_tag="AC16PP_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480232.1"
                     /translation="MRIPKYLALCALVALGGCASWSQETGVDNRWRGAEVPVWVTGET
                     TAEDVMTALGPPSQIIALKDQVVYYYLKETMAGSAYVLILYNNAKMRAEYDRAIFFFD
                     DGGKLTRYAYSNETLSHRGG"
     gene            892..1476
                     /locus_tag="AC16PP_01030"
     CDS             892..1476
                     /locus_tag="AC16PP_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480233.1"
                     /translation="MKVIRLLLGVIVISGANVALGDGHGGDGLVTFSATQKETLTAIV
                     EAIDHETRVVTVRGPRGNAITFTASDEVRNLDQVAAGDLIDAEYVESLDVEVLPGDGS
                     EPGHGVMGAVARTDEGEMPGVAAAEVNVITAKIVAIDLEASTATLEGPDGALEEIEPM
                     NPSNLERVSVGDIVVFTVTQSVALSVREQRADAE"
     gene            complement(1488..2078)
                     /locus_tag="AC16PP_01035"
     CDS             complement(1488..2078)
                     /locus_tag="AC16PP_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HIR6480234.1"
                     /translation="MRMAIGFATLCIVLTLPSEANSAFGKKKSPEERRENITEMREDV
                     LNRLYDERASAKAQVQDSVGYAVFSNTGVNLFLLSTANGAGVAHDNGAGKDIFMNMFS
                     AGGGIGLGVKKFSAVFIFHTRDAFDQFVEEGWNFTGQADAAATTDAEEDEQVGAAGGT
                     VGINDGVTVYQMTEKGLALQATLQGTKYWKNEDLNN"
     gene            2274..2351
                     /locus_tag="AC16PP_01040"
                     /pseudo
     CDS             2274..2351
                     /locus_tag="AC16PP_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024851.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            complement(2383..3645)
                     /locus_tag="AC16PP_01045"
     CDS             complement(2383..3645)
                     /locus_tag="AC16PP_01045"
                     /inference="COORDINATES: protein motif:HMM:NF052063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HIR6480235.1"
                     /translation="MLRLPLLVMLLGCTVLAAAAPAQPVVRVEVNPESVRVGESAKLK
                     VIVLVPTWFARPPVYPTFELANAITRLPPDSSYPTGERVGRETWSGIVRNYRFYPLLG
                     ANYRLSGETITVTYANPGSTPLTVEVAVPEISIRGVVPAGAEGLNPYLAGRRLTLSRA
                     VESQAEPLRVGDAVVVTYAAELDGLPAIFLPPLLPELDVDGVSGYADVPKLGDGPPAR
                     REEKLTLVFDAGGTFTLPGTKLSWWNSESGRIETTSLEDLSFEVTGPLPSASVQTYDE
                     RALLGLAIAVTLVLVLGLIAWKAAPALAHTLQRAAEARRRSEPYAFRQLRRSIRAGNR
                     SAIHADLLAWLKKLNVEHDARAFARVYGNERLVAAIDSLSAHLYGRSQPDADPAELRR
                     GLVAARRRCQRSQGGADRAAGRLPALNP"
BASE COUNT          632 a         1169 c         1231 g          665 t
ORIGIN      
        1 cacgggccag ttggacgaga gcccgagctg gcgttcttca accgcgtacc agtggtacag
       61 gaaggcgtaa ccgtcgttga tcgcgctcat ggcgtgcggt gggcccagcg cctccagcac
      121 atcgtccacg gtggcgtccg cacggtcggc cagcgctcgg gacgcctcgc gtggcggctt
      181 cttgccgacg tcctggtgaa tcatcgagca gccgctcgat gcggcgagca ggatcgcgag
      241 ggtcgcgatg gcgacgatgg gcatcagccg ccccggtggg aaagcgtctc gttgctgtac
      301 gcgtaacggg ttagcttgcc cccgtcgtcg aagaagaaga tggcccgatc gtactcggcc
      361 ctcatcttcg cgttgttgta gagaatcagc acataggccg agccggccat tgtctccttc
      421 aggtagtagt agaccacctg atccttgagg gcgatgatct gggaaggcgg cccgagtgcg
      481 gtcatgacgt cctctgccgt ggtttcgccg gtgacccaga cgggcacctc ggctccgcgc
      541 cagcggttgt ccacaccggt ctcctgactc cagctcgcgc agccgcccag agccacaagg
      601 gcgcacagcg ccagatactt tggaattctc atgatgtcct ctggttccgc cataccggcg
      661 caggcagcga gcttgtcgat cttatcctgc cggcccggcg caattggttt ccagaccata
      721 gcaggagggg atagcggcct ccactcggat tcgatagtga ccggaggggt gaccggaagc
      781 gctggagtca ctcgtgctcg cccctcctcg ctttcgccgc gccgtccgcg ttgcgcgtga
      841 atgtccgtcg ccgatatcat cagccgcgct caattcaagg ggtccactgc catgaaggtt
      901 attcggctgt tgctgggggt tatcgtcatc tcgggggcta acgtcgcact tggcgacggg
      961 cacgggggcg atggcctggt tacgttctcg gccacccaga aggaaacgct cacggcgatt
     1021 gtggaggcca tcgaccacga aactcgggtg gtcactgtca ggggaccccg gggcaacgcc
     1081 atcaccttca ccgcgtcaga cgaggtccgc aatctcgatc aggtggcggc gggggatctc
     1141 atcgacgcgg agtacgtgga gtctctggac gtggaggtgc tgccgggaga tggcagcgag
     1201 ccagggcacg gcgtgatggg cgccgttgct cggaccgatg agggcgagat gcccggcgtg
     1261 gcggcagccg aggtcaacgt gatcacggcg aaaatcgttg ccattgacct cgaggccagc
     1321 accgctaccc tcgaaggtcc cgatggggcg ctagaggaga tcgagccgat gaacccgtcc
     1381 aatctggagc gggtctcggt gggtgacatc gtggtcttca cggtcaccca gtccgttgcg
     1441 ctctcggtac gcgagcagcg ggctgacgcc gagtaggcgt tcaaccgtca gttgttgagg
     1501 tcttcgttct tccagtactt ggtgccctga agggtcgcct gtaacgcgag gcctttctcg
     1561 gtcatctggt acacggtgac gccgtcgttg atcccgacgg tgccgccggc ggcgccgacc
     1621 tgctcgtctt cctcggcgtc agtggtcgcc gcggcatccg cctggccggt gaagttccag
     1681 ccctcctcaa cgaactgatc gaaggcgtcg cgagtgtgaa aaatgaacac ggcgctgaac
     1741 ttcttgacgc cgagaccgat cccgccgccg gcggagaaca tgttcatgaa gatgtctttg
     1801 ccggccccgt tgtcatgtgc gacgcctgcg ccgttggccg tggagagcag gaacagattg
     1861 acgcccgtgt tggagaacac ggcgtaaccc accgaatcct gaacctgtgc cttggccgat
     1921 gctctttcgt cgtagagcct gttcaggacg tcttcgcgca tctcggtgat gttttcccga
     1981 cgctcctcgg ggctcttctt cttgccgaag gcggagttgg cctccgacgg cagggtcagg
     2041 acgatgcaga gcgttgcaaa tccgatggcc atgcgcatgg ttggttcccc tctgatacaa
     2101 aagaatcccg tcacgcagac ggggtgccaa gtataagcgc tcagccgtaa gtggaaacgc
     2161 caattctcag cggcctctgc agccttcatc gtttttggtc gtcttcgaga aagccgggga
     2221 ggtctggtcg tggatcctga agaagtgaac ctctccgccg tcggcgtgca gaagtccccg
     2281 aggaactcga ggtcgacgcg cagtctgccg ccaacgcgtg tccggtgggc gccatcgtca
     2341 tcgagtattg agggccctcg cttcggatct cgaacgatcg gctcaggggt tcagtgccgg
     2401 tagccggccg gcggcgcggt ccgccccgcc ctgacttcgc tgacagcggc ggcgcgcggc
     2461 caccagtcct cgccgaagct ctgccggatc ggcatcgggc tgagagcgcc catagagatg
     2521 ggcgctcagc gaatcgatcg cggccacgag cctttcgttg ccgtaaacgc gggcaaaggc
     2581 gcgcgcgtcg tgttccacgt tcagcttctt caaccaggcg agcagatcgg cgtgaatggc
     2641 cgagcggttg ccggcgcgga tgcttcggcg cagctggcga aaggcgtagg gctccgagcg
     2701 ccgacgcgct tcggcggccc tttgcagggt gtgggcgaga gccggtgcgg ccttccacgc
     2761 gatcagcccg aggacgagga ccagcgtgac cgcgatcgcc aggccaagca gtgcccgttc
     2821 gtcgtaggtc tgcacgctgg ccgacggcag cggtccggtc acctcgaaac tcagatcctc
     2881 gaggctggta gtctcgatcc gccccgactc gctgttccac caggacagct tcgtgccggg
     2941 caaggtgaac gtgcccccgg cgtcgaaaac cagggtcagc ttttcctcgc gccgcgccgg
     3001 aggcccatcg cccagcttcg gcacgtccgc atagccgctt accccatcga cgtcgagttc
     3061 gggcagcaga ggcggcagaa agatcgcggg caggccatcc agctcggccg cgtatgtcac
     3121 cactaccgcg tcgccgaccc ggagcggctc ggcctggctc tcgacggccc gcgaaagggt
     3181 gagccgcctg ccggcgaggt aggggttcaa cccctcggcg cccgcgggaa cgacgccgcg
     3241 gatcgatatc tccgggacgg ccacctcgac cgtgagcggc gtgctgcccg gattggcgta
     3301 cgtcacggtg atcgtttcgc ccgacaaacg atagttggcg ccaagcagcg gatagaagcg
     3361 gtagttgcgg acgatgccgg accaggtctc tctgcccacc cgctcgccgg tggggtagga
     3421 gctgtccggc ggcaggcgcg tgatggcgtt ggcgagctcg aacgtggggt agacgggcgg
     3481 ccgggcgaac caggtgggaa ccagcacgat caccttgagc ttcgccgatt cgccgacccg
     3541 aacgctttct ggatttacct caacgcgcac caccggctgc gctggagccg cagcggccag
     3601 caccgtgcag cccagaagca tcaccaggag cggcaggcgc agcatcacag atcaccccgc
     3661 cggacggctt cgagcagaaa ccgtgaacgc agaaagt
//
LOCUS       DBNHKV010000037         5875 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1210741, whole genome shotgun sequence.
ACCESSION   DBNHKV010000037 DBNHKV010000000
VERSION     DBNHKV010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5875)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1210741"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="AC16PP_01050"
     CDS             complement(<1..813)
                     /locus_tag="AC16PP_01050"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HIR6480236.1"
                     /translation="MTRDDRGNFVDSEGIATEPHELAIRDARQLSPANAMTLALNGFE
                     LRDAPVASYDFLDHQEVIAGYYRDCENIVAEATGARAWAFDHNIRSAGGQAEKRQVRG
                     GQDVQGPAHIVHGDYTLRSAPERLAQLTRPPSVNDTLRDVIPRGQGLIAEHEANQALA
                     DGGRFAIINVWRNIGSVPVATHPLALCDGQNVEPEDLVVFEIHMPDRVGENYWAKYDA
                     RHSFYCYPAMTRDEALLIKQWDSAGLLARSGGAQADHEADGPCTFSFHSAFHD"
     gene            complement(1000..1734)
                     /locus_tag="AC16PP_01055"
     CDS             complement(1000..1734)
                     /locus_tag="AC16PP_01055"
                     /inference="COORDINATES: protein motif:HMM:NF028481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6480237.1"
                     /translation="MITLFRFGPAWDKFGCVSQFVLKVETYLRMAGIEFETRSLGIGF
                     AETAPKGKLPYIDHDGAQVADSSFIIDYLKEQFGDPLDAALSATERARGHTIKRMVEE
                     HVWWLMARERWWAPEAPYWDTPGLLKELDRASYEEVRDDSQRKYVEHGVGAFTEDELD
                     RRGREDLDALATLLGEQTYFLGDRPTSQDATTYAFLWHILNAPYASKLKEAALEHENL
                     VAYTRRISEEWFAEDPMSLEREVSSS"
     gene            complement(2077..2538)
                     /locus_tag="AC16PP_01060"
     CDS             complement(2077..2538)
                     /locus_tag="AC16PP_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6480238.1"
                     /translation="MPTLEERLQYVEDRFALKDALTRYCTRIESLEDVDALLNCFTDD
                     AVFDLTGIDLPRFEGREALRGFFLQVFDDMTHHAHFTTNFAIDEITTDTARCTAYVMG
                     MGVARDGNDVLVYVKYFLDFVKQNGAWKIRNFGEASLMPLPESLTEIHGRD"
     gene            complement(2731..3027)
                     /locus_tag="AC16PP_01065"
     CDS             complement(2731..3027)
                     /locus_tag="AC16PP_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480239.1"
                     /translation="MSIQDLGSIGEFIAALATLATLVYLAVQIRQNTKQVEEGTRAAR
                     ASAVSSGLQLINSNRLAIYSDQDVASIWSRGLEDPTAPDPIDPKRQVVLAALQR"
     gene            complement(3158..3559)
                     /locus_tag="AC16PP_01070"
     CDS             complement(3158..3559)
                     /locus_tag="AC16PP_01070"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR6480240.1"
                     /translation="MSQARAIEIMSTYLNEVVGKGEIGLIEDFTAADFVDHTQTELRG
                     PAALTAHVQNFRGNIPDLRVEVLGISANDDAAFGIWRWHGTPVDPVWGKSATGETIVP
                     SLIGSYFRIENDMLVEYQPFIDAMNVLGQLT"
     gene            3744..4760
                     /locus_tag="AC16PP_01075"
     CDS             3744..4760
                     /locus_tag="AC16PP_01075"
                     /inference="COORDINATES: protein motif:HMM:NF005757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6480241.1"
                     /translation="MGEGLEGRFELGDFKLQSGAVIPDAFLGYATHGTLNEARDNVIV
                     YPTWYTGRHAGAEPYIGPGKALDPERYFIVVPDMFTNGLSSSPSNCAPPLSGVNFPLV
                     TPFDNVVAQYQLLTDNFAITGVELAMGFSMSGQQAYHWGAIYPDFVKRICSICGSAKT
                     SPHNWVYLNAYKTAIESAQGWEDGGAQAWPPGVLPVMASIAATMAWSQDWYREGLYRA
                     TAADTPEEFVSAVTGFFDSWHPADMYAQTRTWMAADVSDNEVFNGDLDAALGAIKARA
                     LIMPCDTDMYFRVADNEAEVAKMPNAELKVIRSSWGHMAGMPGASPADDAFVDAALKD
                     LLKT"
     gene            4804..5661
                     /locus_tag="AC16PP_01080"
     CDS             4804..5661
                     /locus_tag="AC16PP_01080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6480242.1"
                     /translation="MKFGLIPINIGMVDTERMVGLAQLAESLGFESVWTFEHVIVPVD
                     YQSKYPYNDSGKMGAPPETPFIDPLIALTAIAAGTKTIRLGTGVNILSQVNPLYMAKQ
                     AASLDFISNGRFMLGVGIGWLREEFEALGVPFERRGARFDDYVEAMKKVWSGDVVEHQ
                     SDFIDWSGFKSYPLPVQKPHMPVIMGGAAGKIFERTAKLGDGWFAPMGTVDELKPALD
                     KLRAACDAQGRDFSEIEITCMWRPGAGRDAVAALEGIGVSRMVGMIPGLGSDPVAGLT
                     RIAEEFIGA"
BASE COUNT         1104 a         1744 c         1793 g         1234 t
ORIGIN      
        1 gtcgtgaaac gcactgtgga agctgaacgt gcatggtccg tcggcctcgt ggtctgcctg
       61 cgcgccgccg ctccgtgcta gcagccccgc ggaatcccac tgtttgatca gcagcgcttc
      121 gtcgcgcgtc attgccggat agcaatagaa cgagtgcctc gcgtcgtact tcgcccagta
      181 gttctcaccc acacggtccg gcatgtgaat ctcgaacacg acgagatctt cgggctccac
      241 gttctggccg tcgcacagcg ccagcggatg ggtcgcgacc ggtacggatc cgatgttgcg
      301 ccacacgttt atgatggcga agcggccgcc atctgcgagt gcctgattgg cctcgtgctc
      361 ggcaataagt ccttgtcccc gggggatcac gtctctcagc gtgtcattca cgctcggcgg
      421 gcgcgtcagc tgcgcgagcc gttccggcgc ggagcgcagg gtgtaatcgc catgcacgat
      481 gtgcgccggg ccctgcacgt cctgtccacc cctgacctga cgcttctccg cctgtccgcc
      541 cgccgagcgg atgttgtggt cgaaggccca cgcccgggcg ccggtagcct cggcaacgat
      601 gttctcgcag tcacggtagt agccggcgat cacttcctga tgatcgagga agtcgtagct
      661 cgccacgggc gcgtcccgaa gctcgaaacc attgagcgcc agcgtcatgg cattcgccgg
      721 cgacagttgc cgcgcatctc tgatcgcgag ctcgtgaggc tcggtagcaa taccttcgct
      781 gtcgacgaag tttcctcgat cgtctcgggt catgcgtacg cgatcgtttc ggtaaagaga
      841 cgattccgta tctggcgtaa ggaagtggag cacgccagtc aggctggcct cggcgcttgg
      901 catgattttc ctcgaataac ggtcaaccgg gaaagcatac caaccgatgg cggtggcaga
      961 ctatccgaca gggcagggcg gcggtttcgt ggcctgccct cagctcgacg agacctcgcg
     1021 ctccagcgac atcggatctt ccgcaaacca ctcctcgctt atgcgccggg tataggccac
     1081 cagattttcg tgctcgaggg ccgcctcttt gagcttcgag gcgtaagggg cgttcaggat
     1141 gtgccacaag aaagcgtacg tggtcgcatc ctgcgatgtc ggccggtcgc cgaggaagta
     1201 ggtctgctcg cccaggaggg tcgcgagggc atccaggtct tcccggccgc gcctgtcgag
     1261 ctcgtcctcg gtaaacgccc ccactccgtg ctcgacgtat ttgcgctggc tgtcgtcccg
     1321 cacctcctca tagctcgccc ggtcgagttc cttcagaagg cccggagtgt cccagtacgg
     1381 cgcctctggt gcccaccatc gttcacgcgc catcagccac cagacgtgct cttcgaccat
     1441 ccgcttgatc gtgtggcccc tcgcccgctc ggttgcgctg agcgcggcgt ccagcgggtc
     1501 accgaactgt tccttgagat agtcgatgat gaagctggaa tcggcgacct gagcgccgtc
     1561 gtgatcgatg tatggaagct tgcccttggg agccgtctca gcaaagccga tcccgaggct
     1621 ccgggtctcg aactcgatgc ccgccatacg caggtaggtc tcgactttca gaacgaactg
     1681 gctgacgcag ccgaacttgt cccaggccgg gccgaagcgg aacagcgtga tcacagtctc
     1741 tctcccaacg atgcctcccg agcttcgaga ataccgcaat ctatgggcgt gcgccggctc
     1801 cgcaggccat aacacctgaa atcaactgga ccgtccaggt aagagcacac aggcaaaatc
     1861 catggcgctc tcactctata acttgagtcg caacagggag cagctcatga ttggcttgct
     1921 gtttctggct gctttgcagt tcgcactgct ggcgatgttt acgatctccg agcgggcttg
     1981 agccattgaa agagacccgt ggcactgact cagtgggtgc aagccggtca tcttcgagtt
     2041 cacgctccag attagccccg caggctcttg aggcggtcag tctcgtccgt ggatctccgt
     2101 gagggattcc ggaagcggca tcagcgaggc ctcgccgaag ttgcggattt tccacgcgcc
     2161 gttttgcttc acgaaatcca ggaaatattt gacgtacacc agcacgtcgt tgccgtcccg
     2221 ggcgaccccc atccccatga cgtaggccgt gcatctggcc gtgtcggtcg tgatctcgtc
     2281 gatcgcgaag ttcgtcgtga agtgtgcgtg gtgtgtcatg tcgtcgaaaa cctgaaggaa
     2341 gaaaccacgc agcgcttcgc gcccttcgaa acgcggcagg tcgatgcccg tcaagtcgaa
     2401 caccgcgtca tcggtgaagc agttgaggag ggcgtccacg tcctcgaggg actcgatacg
     2461 ggtacagtag cgggtgagtg cgtcctttag cgcgaagcgg tcttcgacat actgaagtct
     2521 ctcttccagg gtgggcatgg caatttctct cctttacggg atgcgcagag cggattccgg
     2581 ccgcggagtc ggccggaagg ccattgtcgt cgaggttcgg gtagcccgcg tcgctgcaac
     2641 ccttcctgcc aaaatactac ggcattcggg cgttgtcagg agtcgctgct gagtatcctc
     2701 gctacttcct cccggaaatc cggcgtgaac tcaccgctgt agtgctgcca gaaccacttg
     2761 cctcttgggg tcgatcggat cgggcgcagt tggatcctcc agaccccgtg accagatgct
     2821 cgcgacgtcc tgatcgctgt agatggccag ccggttcgag ttaatgagct gtaagccgga
     2881 gctgacggcg gaggctctgg ctgcccgggt accttcttcg acctgcttgg tgttttgtcg
     2941 aatctgaact gcaagataga cgagggttgc gagcgtcgcg agagccgcaa tgaactcacc
     3001 gatgctaccg agatcttgga tagacatgtc ctgggccctc aatagagcgg ctgagagaga
     3061 gcgggcaccc cagcgacgcc ggcttcctcg atgatatgaa cggacgacga tttcgctgaa
     3121 acgccgctga accgtccgtt acttgggcgg tctctgctta ggtcagttgc ccgagcacat
     3181 tcatggcatc gatgaaaggt tggtactcaa ccagcatgtc gttttcaatg cggaagtaac
     3241 tgccgatcaa acttggaacg atggtttcgc ccgttgcgga cttgccccat actgggtcga
     3301 cgggggtgcc gtgccagcgc cagataccaa atgcggcatc gtcattcgcg ctgattccga
     3361 gcacctcgac tcgcaggtcg gggatgttgc cgcgaaagtt ctggacatga gccgtcaatg
     3421 cggcgggccc ccggagttcc gtttgtgtat ggtcgacgaa gtcggccgcg gtaaaatcct
     3481 cgatcagacc aatctcgccc ttgccgacga cctcgttcag ataagtggac atgatctcga
     3541 tggctcgtgc ttgactcatt ggacccccct ctctttaagc gcttttgcgc gacgtgttta
     3601 ccggctgcta gtttcccgcg aattgaccag ttggagaagt accaatttcc cgtcgttgca
     3661 ggcggactcg ctcacccgct ttgcaggcgt gcggcaatcc cgtagagttt tgtgggaaga
     3721 acgtgacgtt ttagagagat ccgatgggag aaggtctgga aggtaggttc gagcttggcg
     3781 atttcaagct ccaatccggg gcggtgatac cggatgcgtt tctgggatat gcaactcacg
     3841 gaacgctaaa tgaggcccgg gacaacgtca tcgtgtaccc gacctggtac acgggccggc
     3901 acgccggtgc cgagccttac attggacccg gcaaggcgtt ggatccggaa cgctacttca
     3961 ttgtcgtacc tgatatgttc acgaacgggc tgtcgagttc gccgagcaac tgtgctccgc
     4021 ctctgagcgg agtcaatttt ccgctggtca cgccctttga caacgtcgtg gcccaatacc
     4081 agctcctcac cgataacttc gcgatcaccg gcgtggaact cgctatgggt ttctccatga
     4141 gcggacagca agcctaccac tggggggcca tttacccaga cttcgtgaag cgtatctgct
     4201 ccatctgcgg ctcagcaaag acctctcccc acaactgggt atatctgaac gcatataaga
     4261 ccgcgatcga atcggcacag gggtgggagg acggaggcgc tcaggcctgg ccacccggcg
     4321 tccttccggt gatggcgagc attgcggcca ccatggcgtg gtctcaggat tggtatcgag
     4381 agggcctgta ccgtgcgaca gcggccgata cgccagagga gttcgtgtcc gccgttacgg
     4441 gtttcttcga cagctggcat cctgccgaca tgtatgcgca aacgcgcacg tggatggctg
     4501 cagatgtttc ggataatgag gtgttcaacg gcgatctgga cgccgccttg ggtgcgataa
     4561 aggcccgtgc gctcataatg ccctgcgaca cggatatgta cttccgggta gcggacaatg
     4621 aggcggaagt ggcgaagatg cccaacgccg aattgaaggt gattcgctca agctggggcc
     4681 atatggcagg gatgccaggc gcatctccgg ctgacgatgc tttcgtagac gctgcgctga
     4741 aagatctcct gaaaacctga gcgatgattc ccgttctacc accactactc acgaggaatt
     4801 gagatgaaat tcggactcat acccatcaac atcggcatgg ttgacacgga acggatggtg
     4861 ggcctcgccc agctcgccga aagcctcggg ttcgagtccg tctggacctt cgagcatgtc
     4921 atcgtgccgg tggactacca gtccaagtat ccctacaacg atagcggcaa gatgggcgcg
     4981 ccgccggaaa cgcccttcat tgacccgctg atcgcgctga cggcgatcgc cgctggtacc
     5041 aagaccattc gtctcggcac cggcgtgaac atactctccc aggtcaatcc gctttacatg
     5101 gccaagcagg cggcaagcct cgacttcatt tccaacggtc gcttcatgct cggcgtcggt
     5161 atcggctggt tacgcgaaga gttcgaagcg ctgggcgtgc cgttcgagcg tcgcggcgca
     5221 cgcttcgacg actacgtcga agccatgaag aaggtctggt ctggcgatgt ggtggagcat
     5281 cagagcgact tcatcgactg gagcggcttc aagagctatc cgctgcccgt gcagaagccg
     5341 cacatgcccg tgatcatggg gggtgctgca gggaagatat tcgagcggac ggcaaagctc
     5401 ggcgatggct ggttcgctcc catgggcacg gtcgatgagc taaagccggc attggacaag
     5461 ctccgcgctg cctgcgatgc gcaaggccgg gacttctccg agatcgagat cacctgcatg
     5521 tggcggcccg gggccggcag ggatgctgtc gcagccctcg aaggaattgg cgtctcgcgc
     5581 atggtcggga tgattccggg gctcgggtct gatccggtcg cggggctcac cagaatcgct
     5641 gaggagttta tcggcgcttg agatggggcg acagaccctt cctgcgcgcc tgatctgagc
     5701 gatctctgca tgggagtggc tggagaaccc ggacggcatt tttcatggac ccggaggaac
     5761 taattgggac tttatgttcg aaacggcgat cagttggctg atacccgcct tcgccgtggt
     5821 ggcgctcatc gccttcctcg acatctttgc cggtctgcaa gtggtagcgc accat
//
LOCUS       DBNHKV010000038         2667 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1068895, whole genome shotgun sequence.
ACCESSION   DBNHKV010000038 DBNHKV010000000
VERSION     DBNHKV010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1068895"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1705
                     /locus_tag="AC16PP_01085"
     CDS             44..1705
                     /locus_tag="AC16PP_01085"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011267700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HIR6480243.1"
                     /translation="MYRYDDFDRQFIAERVAHYREQTTRYLAGELSESEFLQLRLRNG
                     LYIQRLAPMLRVAVPYGTLSSGQLRKLAHIARHYDRGYGHLSTRQNMQFNWPELAEVP
                     DILAELAEVDMHAIQTSGSCIRNVTTDQFAGVAADEEVDPRPYCELIRQWSTNHPEFD
                     WLPRKFKIAVTGAASDRAAIAVHDIGLRARREDAEGIVFDVFVGGGLGRTPVIAAEIR
                     KSLPVRHLLTYLEAIMRVYNRYGRRDNKYKARIKILVRAMTAEGFRDRVDAEWAHLKG
                     SESTLTDEEIRRIEAFFTPHEYPPLNDADLGPARLEDPAFAAWLRHNVSPHRVPGYAI
                     VTLSTKVTGVPPGDVSDVQMEAVADWADEYGFGELRLGHEQNFVLPDVPQSRLRELWR
                     RTAALQLGVANVGLISDIVCCPGGDYCSLANAKSIPVAEAIQQRFDDYDYQHDLGELD
                     LNISGCMNACGHHHVGHIGILGVDKKGAEYYQISLGGHSGAGGDEASLGEIIGPSFSR
                     AEVPATIGRILDVYLKRRIDDEPFLHCYRRIGIQPFKDHVYERAA"
     gene            1689..2195
                     /locus_tag="AC16PP_01090"
     CDS             1689..2195
                     /locus_tag="AC16PP_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HIR6480244.1"
                     /translation="MNAQLDYIDQPAAQMVSGPGAVPGPARLVAAEQWTPGSSDGLLL
                     AVDAEPEQRFAGAELIAIDFPAFTDGRGLSLAVLLRTRLGFRGELRAVGDIRADMLHY
                     LYRCGFDSFSMSGEGSPVDADGVTKTTAFDALAPYSDCYQASVRQPRPAYRRVERCIA
                     QELSSPSF"
     gene            2159..>2667
                     /locus_tag="AC16PP_01095"
     CDS             2159..>2667
                     /locus_tag="AC16PP_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR6480245.1"
                     /translation="MHSTGIVESFFLIFTGAAVLATAALYSRQPLLVAYIAVGALLGP
                     HGFKLISDSNLLSEISEIGIIFLLFLVGLDLQPSKLKNMVGESLLTAVGTTIVFFAAG
                     FAMMLAFDFSTSEAVVTGIAVTFSSTIIGIKLLPTTVLHHRHTGEIVVSLLLIQDLIA
                     ILALIVLTG"
BASE COUNT          470 a          908 c          794 g          495 t
ORIGIN      
        1 gcggcgtgcg cttcgcgctg agcgccgatt accagccagc cagatgtatc gatacgacga
       61 cttcgacagg cagttcattg cggaacgcgt tgcccattac cgcgagcaga caacccgcta
      121 tcttgccggc gagctgtccg agagcgagtt tctgcagctc aggctccgaa acggcctgta
      181 cattcagcga ctcgctccaa tgctgcgggt ggccgtcccc tacggcacgc tcagctccgg
      241 ccagctgcgc aagctggccc atatcgcgcg ccactacgac cgcggttacg gtcacctcag
      301 cacgcgtcag aacatgcagt tcaactggcc cgagctcgcc gaggtacccg acatcctcgc
      361 agagctggcc gaggtcgaca tgcacgccat ccagaccagc ggcagctgca tacgcaacgt
      421 gacgaccgac cagttcgccg gcgtcgcggc tgacgaggaa gtcgatccca gaccgtactg
      481 cgagctcatc cgccagtggt cgaccaatca cccggagttc gactggctgc cgcgcaagtt
      541 caagatcgcc gtcaccgggg ctgcttccga ccgagcagcc atcgccgtcc acgacatcgg
      601 cttgcgcgcg cgccgcgagg acgccgaggg catcgtgttc gacgtgttcg tggggggcgg
      661 cctcgggcgc acgcccgtca tcgccgccga gatccgcaag tcgcttccgg tgaggcatct
      721 gctgacctac ctcgaggcca tcatgcgcgt ctacaaccgc tacggccgca gagacaacaa
      781 gtacaaggcc agaatcaaga ttctggtacg ggccatgacc gcggagggtt tccgcgaccg
      841 ggtggatgcc gagtgggccc atctgaaggg ctcggaaagc acgctgaccg atgaggaaat
      901 ccgccgcatc gaggcttttt tcacccccca cgagtatccg ccgctgaacg atgccgatct
      961 gggacccgcg cgcctggagg atcccgcctt tgctgcctgg ctgcgccaca acgtctctcc
     1021 ccaccgggta cccggctacg ccatcgttac gctgtccacg aaggtgacgg gcgtgccccc
     1081 gggagacgtc tccgacgtgc agatggaggc cgtggccgac tgggcagacg agtacgggtt
     1141 cggggagctg cgattgggcc acgagcagaa cttcgtgctg cccgacgtcc cccagagccg
     1201 gcttcgggaa ctgtggcgaa gaaccgcggc gctccagctg ggcgtcgcca acgtcgggct
     1261 gatttccgac atcgtctgct gccccggcgg cgactactgc tctctggcca acgccaagtc
     1321 cattcccgtt gcggaagcca ttcagcagcg tttcgacgat tacgactacc agcacgacct
     1381 gggtgagctc gacctgaaca tctccggctg catgaacgcc tgcgggcacc accacgtggg
     1441 tcacatcggc atcctgggcg tcgacaagaa aggcgcggag tactaccaga tctcgctggg
     1501 cgggcattcg ggtgccggcg gagacgaagc gtctctcggc gagatcatcg gcccatcctt
     1561 ttcccgcgcc gaggttcccg cgaccatcgg caggatcctc gacgtctacc tgaaaaggcg
     1621 catcgacgac gagcctttcc tgcactgcta tcgccgcatc ggcatccaac ctttcaagga
     1681 tcacgtctat gaacgcgcag cttgactaca tcgaccaacc cgccgcgcag atggtgtctg
     1741 gaccgggagc cgtgccgggt ccggcgaggc tggttgccgc cgagcaatgg acgcccggat
     1801 cgtccgacgg tctgctcctc gccgtcgacg ctgaaccgga gcagcggttt gccggggcgg
     1861 aactgatcgc catcgacttt ccggcattca cggacggccg cggactctcg ctggccgtgc
     1921 tgctccgaac gcgtctcggt ttccgaggcg agctgcgcgc cgtgggcgac attcgggctg
     1981 acatgctgca ctacctttat cgttgcggct tcgacagctt ctccatgtcc ggcgagggca
     2041 gccccgtaga cgccgacggc gtgaccaaga ccacggcctt cgacgccctt gcaccctact
     2101 cggattgcta tcaagcttcg gtacgccagc cccgtccagc ttaccgaagg gtcgagaggt
     2161 gcatagcaca ggaattgtcg agtccttctt tctgatattc acgggcgccg cggtgctagc
     2221 caccgcggcc ctgtactcgc gccagccgct gctcgtcgcc tacatcgccg tcggtgctct
     2281 gctgggacct cacgggttca agctgatttc agactctaac ctgctttcgg aaatctccga
     2341 gatcggcatc atctttctgc tctttctcgt gggcctggac cttcaaccca gcaagctgaa
     2401 gaacatggtg ggcgaatccc tgctgaccgc cgtgggcacc acgatcgtgt ttttcgccgc
     2461 gggtttcgcg atgatgctcg ccttcgactt ttccacgagc gaggccgtgg tcaccggcat
     2521 cgcggtgacg ttttccagca ccatcatcgg catcaagctg ctccccacca cggttctgca
     2581 tcaccgtcat acgggcgaga tcgtcgtcag cctcctgctg attcaagacc tgatcgccat
     2641 cctcgctctc atcgtcttaa cgggcta
//
LOCUS       DBNHKV010000039        11668 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1069393, whole genome shotgun sequence.
ACCESSION   DBNHKV010000039 DBNHKV010000000
VERSION     DBNHKV010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11668
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1069393"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /gene="aceK"
                     /locus_tag="AC16PP_01100"
     CDS             <1..1677
                     /gene="aceK"
                     /locus_tag="AC16PP_01100"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HIR6480246.1"
                     /translation="QAITAMAQSRFEGQDWAGVQDAGARRLAIYGEHVRDVVAEVHTL
                     TGGSEIDASLWRKSKQAFTSIISEHYNAELYETFYNSIHREMTDDSVVDDTEMFVRTL
                     FPIPPVQPAEPVFERHAIAGEPEEFVRTLLDGLAINRPWVDRDRDVRNILRSLSEERP
                     EIQDPLRLDVDILKPIFYRNKGAYVVGRLLYKDEGGAQLWPFALALLLDDDGRLYVDT
                     LICDVDELSVMFSFTRAYFMVLTDYPHALVDFLQVLLPNKKRTELYASMGLHKHGKTV
                     FYRDFLDHLSRTDDQFVIAPGTPGMVMTVFTLPSLQTVFKVIKDEFAPQKNITAQQVR
                     DKYYVVKSHDRVGRMADTQEFQNLTFPIDRFDPELVEELRRVAPSSIEISGNEILIRH
                     LYTERLMTPLNLFIDTADEGALREALEEYGNAIKQLAAANIFPGDMLLKNFGVTRHGR
                     VVFYDYDEICYLTDVNFRDIPEPRSPEDEMAAEPWYAVGPHDVFPEEFRRFLFGRRKI
                     KQMFEEMHGELFEPSYWRGLQAAINDGQVMDVFPYRRKRRFGEHGAASRA"
     gene            1694..2365
                     /locus_tag="AC16PP_01105"
     CDS             1694..2365
                     /locus_tag="AC16PP_01105"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6480247.1"
                     /translation="MEQLDRRARERLEKADALIFDFDGTLAPNLDLPDMRRQVVDLTL
                     GRGVPGEIFEHLYIVEIIEAAASWLRRRDRTSAADYHEAAHRLITEFELEAARNTEPF
                     PATRTTLATLRRKGKRLGVVTRNCSTAVRTVFSDIDVYIDAVLARDDVDFLKPDARHV
                     RQALSVLDGTPERAAMIGDGRLDMHVGRDLGMTCVGVLTGSADADALLEAGAHIIVGA
                     LEELL"
     gene            2463..2756
                     /locus_tag="AC16PP_01110"
     CDS             2463..2756
                     /locus_tag="AC16PP_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480248.1"
                     /translation="MHRATQARNAIAPLLDQLIVQLDAEGRASQRAYFARIRRSLLLA
                     RHEADLATPIRELSTSVAVGFVFSNDADVLMSRILEKVDQLAGELEFSRPVIH"
     gene            2753..3631
                     /locus_tag="AC16PP_01115"
     CDS             2753..3631
                     /locus_tag="AC16PP_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6480249.1"
                     /translation="MTSPARDAPITGGCLCGAVRFAARGRPLWTAHCHCESCRRHTSS
                     LLATYAGFPSEDVEFSGDQPALIRSSPQAERGHCGRCGSPLCYRSARWPGETHLFLPS
                     FDDPARFEVEAHVMFDERVAGFDVYDDLPRYATGSPTPIAWGPRPAARVLFLCTGNSA
                     RSIIAQTIANDLRATVNGERVRAHSAGSRPVGEVNSGAMEVLRAHGHDVRGLRSKSWD
                     EYTGDAAPALRWVITLCDSAAGETCPAFASRAGVPVERLHWGLPDPASGAASFEAVYQ
                     TLAARIDELIAGAAPA"
     gene            complement(3633..4847)
                     /locus_tag="AC16PP_01120"
     CDS             complement(3633..4847)
                     /locus_tag="AC16PP_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007943237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6480250.1"
                     /translation="MDFNDTAEEAKFREEVRGWLEVNVPTAEELAGLNYIEAAKLWQK
                     RKYDAGWACIRWPEEYGGRGASAIDQVIWNQEEAKYPDLPQGIFGIGQGMAAPTLMTW
                     ADDEAKARYLPRLASGEDIWCQLFSEPAGGSDLAALRTKAERDGDDWVINGQKIWTSG
                     AHYSDYGILVVRTDPTVPKHKGLSYFYLDMKSPGIEIKPIKQISGDSNFNEVYFTDVR
                     IPDSQRLGAVGQGWQVSLTTLMNERASIGGGGTAVGFDSVFKLAQRVNIGDRPAIEDS
                     QVRAKLADWFCQEAGLRFTGYRSMTALSRGEIPGPENSIGKLVGAPKTQDMASFAIDL
                     MEMTGAIWDKEFAEEAGLFQGTYMAIPGLRIAGGTDEIMANIIAERVLGLPQEPRMDK
                     GIPFNEVPTGSG"
     gene            complement(4857..5957)
                     /locus_tag="AC16PP_01125"
     CDS             complement(4857..5957)
                     /locus_tag="AC16PP_01125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6480251.1"
                     /translation="MNFEFNEDQNLLREQARGFLADNCSPAVVRAVLEGDASYDADLW
                     RKIVEMGYTATVIPEEFGGLGLSYLELSVIAEELGRVAAPVPFSSSVYLATEAILLAG
                     SREQKETWLPRLAAGEVIGTFAFGESHGRPTAENLTARVTDGKLSGTKLPVPDGGAAD
                     VAVVVASNGLYLVDLAQDGVAVTDVPTLDPTRGHSRIEFAGVQAEPLGDPADGWSLTE
                     KVLDRAAVLFAWEQVGGSEASLEMAKEYALGRYAFGRPIASYQAIKHKLANMYVKNTL
                     AKSNCYYGAWALNTDASELTLAAATARVSATQAYYFASKENIQTHGGMGYTWEFDCQF
                     YYRRAKLLSVNIGSEAHWQDRLITAVEQSNAA"
     gene            6037..7962
                     /locus_tag="AC16PP_01130"
     CDS             6037..7962
                     /locus_tag="AC16PP_01130"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HIR6480252.1"
                     /translation="MDYTELGFIRVAAVAPELSIAEPATNAEKIVEHLTALEGRAVTI
                     ALFPELALTGYSCEDLFFSEQLHAAARSALLMLARSAGEAVCVVGTPYLLEDGRLLNC
                     AAVIADGRVRAMIPKIAQPNYGEFYERRWFVSGAGVDVSVDDPELGSFQVATNQLMRI
                     GNMRFAVEICEDLWAPEPPGIRHSLAGAELVVNLSASNELVAKADYRRDLVHMASARG
                     ICGYVYASSGPLESTKDLVFGGHLIACENGQLLGESPRYAFEPQTLITDIDIAKLRHD
                     RMQNTTFTNAPRPAPYHVVDVLQRPRPIAALERVYTAHPFVPDDEAQFEARAAEILDI
                     QTTGLARRCLAANTETLVIGLSGGLDSTLAFLVCLDTLDKIGRPRQSIQAITMPGPGT
                     TEHTLESARGLCAAAKVALKEIPIHEAVAQHLDALEHTGRDDLVFENTQARERTQILF
                     DYANKVSGIVVGTGDLSELALGWCTYNADHMASYNVNASVPKTMMAYLVRWYAKHRAG
                     EALAGVLQRVLQTPISPELIAPDTAGSISQETEAIIGPYELHDFFLFHFLRNGAGPAK
                     IFHLARLAFTGSYADDEIKRWLRLFFQRFFTQQFKRTTLPPGPKVGSVSQSPRGDRRM
                     PDEAGVQRLLADIDDLG"
     gene            8017..8943
                     /locus_tag="AC16PP_01135"
     CDS             8017..8943
                     /locus_tag="AC16PP_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6480253.1"
                     /translation="MRYLSLSITGLALLAAIGTAHAADAPFCGDEGVWVQILGAGGPE
                     LDDGQAGPSYLIWRDNHARLLVDAAPGSSAAFDRAGAEFEDLDAIAFTHLHADHSADF
                     PAFIKGSYFAERDRPLTVLGPSGEGVFPDTVTFVERMIGKDGAYPYLADFLTYGSAGG
                     YKVNARNVPSTGQRRWSRFGTEHIRLSAIPVHHDIVPAVAWRVEVGDKTIVFTGDFSN
                     QKNVIPSFAKDADALVVSHAIPETARGAARELHAIPSQLGQIAAQANARMLILGHRMN
                     RTRGRETQSREAIDKHYQGSVIFANDMECWGL"
     gene            8978..9625
                     /locus_tag="AC16PP_01140"
     CDS             8978..9625
                     /locus_tag="AC16PP_01140"
                     /inference="COORDINATES: protein motif:HMM:NF017819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HIR6480254.1"
                     /translation="MKGTGDSPAATAEHEPLLTCWFGPLEAGFADAAHRERWFAPTRS
                     FDAALAERFAPLVATAAAGGLDEWQQTPRGRLAYVLVCDQLPRNIYRGTARAFATDRR
                     AVAAAREAVRIGVDLALALEERAFLYLPFQHSEDILDQHLAVGLFTALRDASPKGYRD
                     LTGGYLRSAQQHRDLIERFGRFPHRNAALGRASTTEEAAYLEDGKTFGQAADAKG"
     gene            complement(9655..10944)
                     /locus_tag="AC16PP_01145"
     CDS             complement(9655..10944)
                     /locus_tag="AC16PP_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016982861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6480255.1"
                     /translation="MTLRRLAPLLLVLNAAGSLAEVSPEIRRGAEAVEAQVIAWRRDI
                     HAHPELSNREFRTAALVAEHLDSLGMEVTTGTAHTGVVGVLRGGLPGPVVALRADMDG
                     LPVEERTGLPYASKVRSTYNGLDVGVMHACGHDNHVAILMGAATVLSGMRERLPGTVK
                     FVFQPAEEGPPAGEDGGARMMVQEGVLEDPDVEAIFGLHISQADTVGFANYRPGGAMA
                     SAQRFEITITGRQTHGARPWQGVDPIVAGAHVVTALQTIVSRQVDISKAPAVVTVGSF
                     HGGVRNNIVPDSVKLEGTIRTFDADMRADIHSRIRRIATTVAESMGATADVYINDGVP
                     VTYNDPALTEAMLPTLHKVYGADRVRYALPITGAEDFSFFQEQVPGFYFFIGGRPADM
                     AAEDAIPNHSPLFFVDEGALKLGVQAMSQLAVDYLNR"
     gene            11046..>11668
                     /locus_tag="AC16PP_01150"
     CDS             11046..>11668
                     /locus_tag="AC16PP_01150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6480256.1"
                     /translation="MEFDIAATDDLLATTRAVRKRLDLTRPVPREIINECLELAVQAP
                     TGSNSQGWRWVVVDDAAKRKALADLYREAAGGYLSGAEEQANESGNQQTQRVFSSAIY
                     LMEHLHEVPVHVIPCLEGRPPEDAPPITYAGVYGSIYPAVWSFQLALRARGLGSTLTT
                     LHLFKEREAAELLNIPDNVMQVALLPVGYTVGTDFKRADRPPVAGIT"
BASE COUNT         2011 a         3972 c         3712 g         1973 t
ORIGIN      
        1 caggccatca ccgccatggc ccaaagccgt ttcgaaggcc aggactgggc tggcgttcag
       61 gacgcggggg cgcgtcgcct ggccatctac ggggagcacg tgcgcgacgt ggtcgctgag
      121 gtccatacgc tcacgggagg gtccgagatc gacgcttcgc tgtggcgcaa atccaagcag
      181 gcgttcacat cgatcatcag cgagcactac aacgccgagc tctacgagac gttctacaac
      241 tccattcatc gcgagatgac cgacgacagc gtagtcgacg acacggagat gttcgttcgt
      301 accctgttcc ccatcccgcc ggtgcagcca gccgagccgg tcttcgaaag acacgccatc
      361 gccggcgaac ccgaagagtt cgtgcgcacg ctgctcgacg gtctggccat caaccggcca
      421 tgggtcgacc gggaccgcga cgtgcggaac atactgcgct ctctttccga ggagcgcccg
      481 gagatccagg acccgctcag gctggacgtg gacatcctga aacccatctt ctacagaaac
      541 aagggcgcat acgtggttgg ccggctgctc tacaaggacg agggcggagc acaactgtgg
      601 ccatttgcgc tggccctgct cctggacgac gacgggcgcc tctacgtgga cacgcttatc
      661 tgcgatgtgg acgagctgtc cgtcatgttc agcttcaccc gcgcctactt catggtcctg
      721 acggactacc ctcacgcgct ggtggacttc ctgcaggtcc ttctgcccaa caagaagcgc
      781 accgagctct atgcctccat gggacttcac aagcacggta aaacggtctt ctatcgtgac
      841 tttctggatc atctgagccg cacggacgat cagttcgtga tcgcgcccgg aacgccgggc
      901 atggtgatga ccgtgttcac gttgccctcc ctgcagaccg tgttcaaggt gatcaaggac
      961 gagttcgcgc cccagaagaa catcaccgcc cagcaggtcc gggacaagta ctacgtggtg
     1021 aagtcccacg atcgggtcgg ccgcatggcc gatacccagg agtttcagaa cctcaccttc
     1081 cccatcgacc ggttcgatcc ggagctggtg gaggaactca ggcgcgtagc accctccagc
     1141 atcgagattt ccggaaacga gattctgatc cgccacctgt acacagagcg gctgatgacc
     1201 cctttgaacc tgttcatcga cacggccgat gaaggggccc tgcgcgaagc gctggaagag
     1261 tacggcaacg ccatcaagca gctggctgct gccaacatct ttcccggcga catgctgctc
     1321 aagaacttcg gcgtcacccg gcacggccgc gtggtgttct acgactacga cgagatctgc
     1381 tacctcaccg acgtcaattt ccgggacatt cccgagccac gctcgcccga agacgagatg
     1441 gcggccgagc catggtatgc agtggggccc cacgacgtgt ttcccgagga gtttcgccgt
     1501 ttcctgttcg gccgacggaa gatcaagcag atgttcgagg aaatgcacgg cgagctgttc
     1561 gagccttcct actggcgcgg cctgcaggca gccatcaacg acggtcaggt gatggacgtc
     1621 tttccgtatc ggcgcaagag gcgtttcggc gagcacggcg ccgcgagccg ggcctgacac
     1681 ccaggggaga ccggtggagc agctggacag gagggcccgc gagcggctgg agaaggccga
     1741 cgccctgatt ttcgacttcg acggcaccct ggctcccaac ctggatcttc ccgacatgcg
     1801 ccggcaggtg gtggacctga ccctcggacg cggcgtgcct ggggagatct tcgagcatct
     1861 ctacatcgtg gagatcatcg aggcggccgc gtcgtggctg cggcgacgcg atcgcacttc
     1921 ggccgccgac tatcacgaag ccgcccatcg gctaatcacc gagttcgagc tggaggctgc
     1981 ccggaacacc gagccgttcc cggccacgcg gaccaccctc gctactctgc ggcggaaagg
     2041 gaagcgcctt ggcgtggtaa cgcgcaactg ctcgacggcg gtgaggaccg tctttagcga
     2101 catcgacgta tacatcgacg ccgtcctggc acgcgacgac gtggactttc tcaaacccga
     2161 cgcccggcac gtgcgccagg cgctgagcgt cctcgacggg acccctgagc gagcggccat
     2221 gatcggcgac ggccgactcg acatgcacgt cggtcgggat ctgggcatga cgtgcgtggg
     2281 cgtgctcacc gggtccgccg acgcggacgc cctgcttgaa gcaggcgccc acatcatcgt
     2341 gggcgcgttg gaggagctgc tgtgacgtcg cttccggcac cgggcgcgac aggctggcct
     2401 tccgcggcga ctgggttcag aattgcgggc agtcgcgtcg gtcctggaac ctggagaagg
     2461 agatgcatcg agccacgcaa gcacgcaatg ccatcgcccc tctgctggac cagctcatcg
     2521 tccagctgga cgccgagggc cgggcttccc aacgcgccta cttcgctcga atccgccgtt
     2581 ccctgcttct tgcacgacac gaagccgacc tagccacgcc cattcgcgag ctgtccacgt
     2641 ctgttgcggt aggcttcgtt ttctcaaacg acgccgacgt tctgatgtcc cggatcctcg
     2701 agaaggtcga tcagctggcc ggcgagctgg agttttcccg gcccgtaatc cattgacgag
     2761 cccggcccgc gacgcgccga tcaccggcgg gtgcctctgc ggcgcggtcc ggtttgccgc
     2821 cagaggtcgt cccctctgga cggcccactg tcattgcgaa tcctgcagac ggcacaccag
     2881 ctccctgctc gccacctacg cgggctttcc gagcgaggac gtagagttca gcggggacca
     2941 gcccgccctg atccgttcgt cgccccaggc cgagcgcggg cattgcggcc gctgcggttc
     3001 cccgctgtgc taccgctcgg cgcgctggcc gggtgagacg cacctgttcc tgcccagctt
     3061 cgacgatccg gctcgattcg aagtcgaggc ccacgtcatg ttcgacgagc gcgttgccgg
     3121 gttcgacgtg tacgacgatc tgccgcgcta cgccaccgga tccccgacgc ccatcgcgtg
     3181 gggtcccagg ccggccgcga gggtgctgtt cctgtgcacg ggaaactcgg cacgaagcat
     3241 cattgcccag accatcgcca acgacctgcg agcgaccgtg aacggagagc gggtgcgtgc
     3301 ccacagcgcc ggcagccgcc ccgtcggaga ggtgaattcg ggtgccatgg aggtgctccg
     3361 ggcccacggt cacgacgtgc gcggcctgcg cagcaaatcc tgggacgagt acacgggcga
     3421 tgcggcgccc gctctgcggt gggtcatcac gctgtgcgac agcgcggcag gggaaacgtg
     3481 ccccgcgttt gcctcgcggg ccggcgtgcc cgtcgagcgg ctgcactggg gcctgccgga
     3541 tccggcatcc ggggcggctt ccttcgaggc cgtgtaccag acgctggccg cccgcatcga
     3601 tgagctgatc gcgggcgcgg cgcccgccta gatcagcctg atcccgtggg gacctcgttg
     3661 aacgggatcc ccttgtccat tctcggttcc tgaggcagcc ccagcacccg ctcggcgatg
     3721 atgttcgcca tgatctcgtc ggtgccgccg gcgatcctca agcccggaat ggccatgtag
     3781 gttccctgga acaacccggc ctcctcggcg aactccttgt cccagatcgc gcccgtcatt
     3841 tccatcaggt cgatggcgaa agacgccatg tcctgtgtct tgggtgcgcc gaccagcttg
     3901 ccgatggagt tttccggtcc cgggatctcg ccccgggaaa gcgccgtcat ggagcggtag
     3961 ccggtgaagc gcagaccggc ttcctgacag aaccagtcgg caagcttcgc ccgcacctgg
     4021 gagtcctcga tggccggcct gtcgccgatg ttgacccgct gggcgagctt gaaaacggag
     4081 tcgaagccga cggcggtccc gccaccgccg atggaagccc gttcgttcat cagcgtggtc
     4141 aacgaaacct gccagccctg acccacggcg cccaggcgct ggctgtcggg aatcctcacg
     4201 tccgtgaagt agacctcgtt gaagttcgag tcgccggaaa tctgcttgat cggcttgatc
     4261 tcgatgcccg gggatttcat gtccaggtag aaatagctca gccccttgtg cttcggcacc
     4321 gtggggtcgg tcctgaccac gaggatcccg tagtctgagt agtgcgcgcc cgaggtccag
     4381 atcttctggc cgttgatcac ccagtcgtcg ccgtcacgct ccgccttggt cctgagcgcc
     4441 gcaaggtccg agcccccggc gggctcgctg aacagctggc accagatgtc ctcgccgctg
     4501 gcaaggcgcg gaagatagcg ggccttggcc tcgtcgtccg cccaggtcat cagcgtgggg
     4561 gcggccatgc cctggccgat gccgaatatg ccttgcggca gatcgggata cttggcttcc
     4621 tcctggttcc agatgacctg atcgatggct gaagcgccgc gcccgccgta ctcctccggc
     4681 cagcgaatgc aggcccagcc ggcgtcgtac ttgcgcttct gccacagctt ggccgcctcg
     4741 atgtaattga gccctgcgag ctcctcggcg gtgggcacgt tgacttcgag ccagccgcgc
     4801 acttcctctc tgaattttgc ttcttccgcg gtgtcgttga aatccatgac tgctccttat
     4861 gccgcgttgc tctgctcgac ggcggtgatc aaacggtcct gccagtgggc ctcgctgccg
     4921 atgttcacgg acagcagctt cgcccggcga tagtagaact gacagtcgaa ttcccaggtg
     4981 tatcccatgc cgccatgggt ctggatgttt tccttggagg cgaagtagta ggcctgagtg
     5041 gcggacaccc gcgccgtcgc cgcggccagg gtcagctccg atgcgtccgt gttgagcgcc
     5101 cacgccccgt agtagcagtt agacttggcc agggtgttct tcacgtacat gttggccagc
     5161 ttgtgcttga tggcctgata agaggcgatg gggcggccga aagcgtagcg cccgagagcg
     5221 tattccttgg ccatctccag cgacgcttcc gagccgccca cctgctccca ggcgaacagc
     5281 acggcagccc gatcgagcac cttctcggtg agcgaccaac cgtcggcggg gtcgcccagg
     5341 ggctccgctt gaacgcccgc gaactcgatg cggctgtggc cgcgggtcgg gtccagggtc
     5401 ggaacatccg tcaccgcaac cccgtcctgg gcaagatcca ccaggtacag cccgttgctc
     5461 gccacgacca cggcaacgtc tgccgccccg ccgtcgggca ccggcagctt ggttccggag
     5521 agcttgccgt ctgtcacgcg ggcggtcagg ttctccgcgg tggggcgccc atggctctcg
     5581 ccgaaggcga acgttccgat cacctcgccg gcggccagtc gcggcagcca ggtttccttc
     5641 tgctcccggg agccagccag caggatggct tcggttgcca ggtaaacgga tgacgagaac
     5701 ggcaccggag cggcaacgcg accgagctcc tcggcgatca cggacagctc caggtaggag
     5761 agcccgagcc ccccgaactc ctcgggaatc accgtggccg tgtaacccat ctccacgatc
     5821 ttccgccaga ggtcggcatc gtaggaggca tcaccctcca gcacggctct gaccaccgcc
     5881 ggagagcaat tatcggcgag aaagccccgc gcctgctccc gcaagaggtt ctggtcttcg
     5941 ttgaattcga agttcatgag catccccaga atcgccaaga acgggcgatt ttccatgctt
     6001 tgggtttaaa acgcgagccc agttaaactg tgcaccatgg actacaccga actgggcttc
     6061 atccgcgttg ccgccgtggc gccggaactc tccatcgccg aaccggccac caacgcggag
     6121 aaaatcgtcg agcacctgac agcgctcgag ggacgcgctg taaccattgc gctgttcccc
     6181 gagcttgcgc tgaccggcta ctcgtgcgag gacctctttt tcagcgaaca gctccatgcc
     6241 gccgcccgtt cggccctcct catgctggcc cgatccgccg gcgaggccgt ctgcgtcgtc
     6301 ggcacgccgt acctgctgga ggacggcagg ctgctgaact gcgcggccgt catcgccgac
     6361 ggcagggtcc gcgccatgat ccccaagatc gcccagccga attacggcga gttctacgag
     6421 cgccgctggt tcgtctccgg cgcaggggtg gacgtcagcg tcgacgaccc tgagctcggg
     6481 tccttccagg tggctaccaa ccagctgatg cgcatcggaa acatgcggtt cgcggtggag
     6541 atttgtgagg acctctgggc gccggagccg cccggcatcc gtcacagcct ggccggagcc
     6601 gagctggtcg tgaacctgtc cgccagcaac gagctcgtgg ccaaagccga ctatcgaagg
     6661 gatctggtgc acatggccag cgccagggga atctgcggct acgtctacgc gtccagcggc
     6721 ccgctggagt cgaccaagga cctggtgttc ggcgggcacc tcatcgcctg cgagaacggc
     6781 cagctcctgg gcgagagccc gcgttacgcg ttcgagcccc aaacgctgat cacggacatc
     6841 gacatcgcca agctgcgtca cgaccggatg cagaacacga cgttcaccaa cgcgccgcgc
     6901 ccggcgccct accacgttgt ggacgtcctg cagcgccccc ggcccatcgc ggctctggag
     6961 cgcgtctata cggctcaccc gttcgttccc gacgacgagg cccagttcga ggcccgggcg
     7021 gccgagatcc tcgacatcca gacgacgggg ctcgcccgcc gatgcctcgc tgcgaatacc
     7081 gaaactctcg tcatcggcct gtcgggcggg ctcgactcca ccctggcttt cctcgtctgc
     7141 ctggacaccc tggacaagat cggccgtccc cgccagtcca tccaggccat caccatgccg
     7201 ggccctggga ccaccgagca cactctggag tcggcgaggg ggctctgcgc cgccgcgaag
     7261 gtggcgctca aggagatccc cattcacgag gcggtggcgc agcacctcga cgcgctggag
     7321 cacaccggac gtgacgatct ggtgttcgaa aacacccagg ctcgggaacg cacccagatt
     7381 ctcttcgact acgccaacaa ggtttcgggc atcgtcgtag gaaccggcga cctgtcagag
     7441 ctggcgctcg gctggtgcac gtacaacgcg gaccacatgg cgagctacaa cgtcaacgcc
     7501 agcgtgccga aaaccatgat ggcctaccta gtgcggtggt acgccaaaca ccgcgcgggg
     7561 gaagcgctgg ccggcgtcct gcagcgcgtc ctgcagacgc ccatctcgcc ggagctgatc
     7621 gcgcccgaca ccgcgggcag catcagccag gaaaccgagg ccatcatcgg cccctacgag
     7681 ctgcatgatt tcttcctctt ccacttcctg agaaacgggg ccggtccggc aaagatcttt
     7741 catctggcca ggctggcatt taccgggagt tacgcggacg acgagatcaa acgctggctg
     7801 aggctattct tccagcgctt cttcacccag cagttcaagc gcaccaccct tcccccgggt
     7861 ccaaaggtgg gctccgtgag ccagtcgccg cggggcgaca ggcggatgcc cgacgaggcc
     7921 ggcgtccagc gcctgcttgc cgacatcgac gacctgggct gaacgccagg ggccccttga
     7981 acgaaacgac ccgataaccg aaccgaggac ttcagcatgc gttacctcag cttgagcatc
     8041 accggcctgg cactgctggc cgctatcggc accgctcatg ccgccgatgc cccgttctgc
     8101 ggcgacgagg gagtctgggt gcagatcctc ggcgcaggcg ggccggagct cgacgacggc
     8161 caggccggac ccagctacct gatatggcgc gacaaccacg cccgcctgct ggtggatgcg
     8221 gcgcccggtt cttccgccgc cttcgaccgc gccggggccg aattcgagga tctggacgcc
     8281 atcgcgttca cgcacctgca cgccgaccac tcggcggatt tccccgcctt catcaaagga
     8341 tcctactttg ccgagcgcga ccggccactg acggtgctcg gcccgtccgg cgagggcgtc
     8401 ttccccgaca ccgtgacgtt cgtggaacgg atgatcggca aggatggcgc gtacccctac
     8461 ctcgccgatt ttctgaccta cggctccgcg ggaggctaca aggtgaacgc gcgaaacgtg
     8521 ccttccaccg ggcagcgtcg ctggtcgcgg ttcggcaccg aacacatcag gctgtctgcc
     8581 atccccgttc atcacgacat cgttcccgcc gtggcctggc gggtggaggt gggcgacaag
     8641 accatcgtct tcaccgggga tttcagcaac cagaagaacg tcatcccctc atttgccaag
     8701 gacgcagacg cgctggtggt cagccacgcg atcccggaga cggcgcgagg cgccgcgcgg
     8761 gagctgcacg ccatccccag ccagctcggc cagatcgccg cccaggccaa cgcacgcatg
     8821 ctgattctcg ggcaccgcat gaaccggacc cgcggtcgtg agacccagag ccgcgaggcc
     8881 atcgacaagc actaccaggg ctcggtgata ttcgccaacg acatggagtg ctgggggctt
     8941 tgagccactc gcgtcagggg cggggcgaag cgctcgggtg aaagggacag gcgactcgcc
     9001 ggcggccaca gccgagcacg aaccgcttct tacctgctgg ttcggaccgc tcgaggcggg
     9061 cttcgcggac gcggcccatc gggagcgctg gtttgccccg acccgctcgt tcgacgccgc
     9121 cctcgccgaa cgcttcgcgc cgctggtggc caccgccgcc gcgggcgggc tcgatgagtg
     9181 gcagcagacg ccgaggggcc gcctggccta cgtgctggtc tgcgatcagc tgccgcgcaa
     9241 catctacagg ggcacggccc gggccttcgc caccgaccgc cgggccgtgg ccgccgcccg
     9301 ggaggcagtt cgcatcggcg tggatctcgc cctcgccctg gaggagcggg ccttcctgta
     9361 cctgcccttc cagcactccg aggacatcct ggaccagcac ctggccgtgg gccttttcac
     9421 cgccctgagg gacgcaagcc ccaagggcta ccgcgatctg acgggcggat atctgcggtc
     9481 cgctcagcag caccgcgacc tcatcgagag attcggccgc tttccgcacc gcaatgccgc
     9541 cctcggacgc gcttccacca ccgaagaggc ggcctacctc gaggacggca agacgttcgg
     9601 acaagccgct gacgccaagg gctgacgctc ttgcagcctg tcctttgccg taaatcagcg
     9661 attcagataa tccaccgcga gctggctcat ggcctggacg ccgagcttca gcgcgccttc
     9721 gtccacgaag aacagcggcg agtgattggg aatcgcgtcc tccgccgcca tgtccgcggg
     9781 gcgccctccg atgaagaaat agaacccggg cacctgctcc tggaagaacg agaagtcctc
     9841 ggccccggtg atgggcaatg cgtagcgaac ccgatcggca ccgtagacct tgtgcagggt
     9901 gggcagcatg gcctcggtta gcgcaggatc gttgtaggtc accgggacgc cgtcgttgat
     9961 gtagacgtcc gccgtcgcgc ccatggattc cgccacggtg gtcgcgatgc ggcggatcct
    10021 tgaatgaatg tccgcccgca tgtcggcatc gaaggtacgg atggtgccct cgagcttgac
    10081 ggagtccggg acgatgttgt tgcgcacgcc accgtggaac gagccgacgg tgaccaccgc
    10141 gggcgccttg ctgatgtcca cctgacggct gacgatggtc tgcagcgccg tgacgacgtg
    10201 agcaccggcg acgatggggt cgacgccctg ccagggccgg gccccgtggg tctggcggcc
    10261 ggtgatggtg atctcgaagc gctgggcgct ggccatggcg ccgccgggcc gatagttggc
    10321 aaaacccacg gtatccgcct ggctgatgtg cagaccgaag atggcctcca cgtcgggatc
    10381 ctcgagaacg ccctcttgca ccatcatcct cgccccaccg tcctcaccgg caggcgggcc
    10441 ctcctcggcc ggctgaaaaa cgaacttgac cgtgccgggc agccgctcgc gcatgccgct
    10501 gagaaccgta gccgcgccca tgaggatggc cacgtgattg tcatgcccac aagcgtgcat
    10561 caccccgacg tccaggccgt tgtaggtcga gcggaccttg gaggcatagg gcaatcccgt
    10621 gcgctcctct acgggaagac cgtccatgtc ggcgcgcagg gccaccactg ggcctggcag
    10681 tccgcctcgc agcacgccca ccacccccgt atgggcagtg ccggtggtca cctccatgcc
    10741 gagactgtcc aggtgctcgg caacgagggc cgccgtgcgg aactcgcggt tggaaagctc
    10801 tggatgggca tgtatgtccc ggcgccaggc aatgacctgg gcctccacgg cttccgctcc
    10861 ccggcgaatc tccggcgaga cctcggcgag ggagccggca gcgttgagca cgagaagtag
    10921 aggagcgagt ctgcgcagcg tcatctggga ttcctcagcc gtccccggtt tgggcgagct
    10981 taatgcgcct caagcatgcg cacaaagtga gttaaacttc ccgggcattc agagggggaa
    11041 caagcatgga attcgatatc gccgccacag acgacctgct ggccacgacc cgggccgtgc
    11101 gcaagcggct cgacctgacg cggcccgtgc cgcgcgagat catcaacgag tgcctggagc
    11161 tggccgtcca ggcgccgacg gggtcgaact cccagggctg gcgctgggtc gtggtcgacg
    11221 acgcggcgaa gcgcaaggca ttggcggacc tgtaccggga ggccgcgggc ggctacctgt
    11281 cgggcgcgga ggagcaggca aacgaatccg gcaaccagca gacccagcgg gtgttcagct
    11341 cggccatcta cctcatggaa cacctccacg aggtgcccgt ccacgtcata ccgtgcctgg
    11401 aaggacgtcc tccggaagac gctccgccca ttacctacgc gggcgtctac gggtccatct
    11461 acccggccgt gtggagcttt cagctggctc tgcgtgcgcg ggggctcggc tccacgctga
    11521 cgacgctgca tctcttcaag gagcgggagg ccgccgagct gctgaacatt ccggacaacg
    11581 tgatgcaggt tgcgctgctt ccggtggggt acaccgtcgg caccgacttc aagcgcgccg
    11641 atcggccgcc ggtggcgggc atcaccca
//
LOCUS       DBNHKV010000040         2584 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1919136, whole genome shotgun sequence.
ACCESSION   DBNHKV010000040 DBNHKV010000000
VERSION     DBNHKV010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1919136"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="AC16PP_01155"
     CDS             complement(<1..285)
                     /locus_tag="AC16PP_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6480257.1"
                     /translation="MAHLAVEGDRRIYFEHYPGAREVTVVLSHGWGMACRVWDNTTAC
                     LQDAGVGVVAYDHRNCGRSDKDFRDVSISALGDDVVALCDHLGLGGVVVNG"
     gene            complement(361..2403)
                     /locus_tag="AC16PP_01160"
     CDS             complement(361..2403)
                     /locus_tag="AC16PP_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR6480258.1"
                     /translation="MARLTHLPTDLDGGERVRGFAFFIAGLLTIWGASGCSAPEDARD
                     EAPIEGPTAAAPTETGGASDEAGEGAVLASTDEFQVGLGDLGDREALPGAALYADNCA
                     ACHDGSVPKAPLFTWLEMMSARTLLGAMNDGIMAAQAADLTADERVHIVEYLTRQRYV
                     AGAVDDLADAHCEAPRAAFDRDRPAPRVGWGHDTRRFVPGDVAGLSAADVPDLELAWA
                     FVFPDALRARSQPAIGYGAVFVGSQDGRVYALDLETGCVRWHYQAAAEVRTGIVVAEI
                     HGRPMAFFGDILAWVHAVDALTGEPVWKRKADEHPGATLSGTPALHEGVLYVPVSSLE
                     IIAAADPAYECCTFRGSVLAVNAANGSERWRSYSIPEPPTVAQKTSAGTNVYAPSGAP
                     IWASPAIDAKRNRLYVGTGENYSTPADGNSDAVLAIDLTTGERIWTRQMTEGDAWNVA
                     CMMADNPNCPPEDGPDFDLGSSQMIVDLADGRQVLVGGHKIGAAFAFDLDAVKGDDPE
                     PLWWTRVGRGSIQGGVHFGMAADGTTIYVPINDMNDTRNGDVLDPEAARPGVHAVNAA
                     DGKVLWSRVQPDRCGDDRPFCDPGISAATTAVPGVLFAGHLDGVIRAYERDTGNVLWS
                     FDSAREFTGVDGRVGRGGGMSGAGPAVGEGYVVVNSGYGLYFHEPGNVLLAFRPSR"
     gene            2461..>2584
                     /locus_tag="AC16PP_01165"
     CDS             2461..>2584
                     /locus_tag="AC16PP_01165"
                     /inference="COORDINATES: protein motif:HMM:NF016750.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480259.1"
                     /translation="MSEVRTAICRFCHSFCGVNVTLDDGRIIKVLGDIENPMYHG"
BASE COUNT          447 a          917 c          840 g          380 t
ORIGIN      
        1 gccgttgacg accaccccac ccagcccgag atgatcgcag agcgccacca cgtcgtcgcc
       61 taatgccgag atggagacgt cgcggaagtc cttgtcagag cgtccgcagt tgcgatggtc
      121 gtaggcaacc acgccgacgc cggcgtcctg aaggcacgcc gtggtgttgt cccatacccg
      181 gcaggccatt ccccatccgt gagagagcac caccgtcacc tcgcgggcac ccggatagtg
      241 ctcgaaatag attcgtctat cgccttctac ggccagatgt gccacgactt cccccatcaa
      301 aaaaccaagg ctcatcgagc ctaaggatcc tcggcattca gcgcaaccgg ggctgcccga
      361 tcagcgggac ggtcgaaagg ccagcagcac gttgccgggc tcgtggaagt aaaggccgta
      421 gcccgaattc accaccacgt agccctcgcc caccgcaggc cctgccccgc tcatgccccc
      481 gccgcgacca acccgcccgt ccaccccggt gaactcccgg gcggaatcga agctccacaa
      541 gacgttcccg gtgtcccgct cgtaggcacg aatgacgccg tcgagatggc ccgcgaagag
      601 gacgcccggg accgccgtgg tggccgccga gatgcccggg tcgcagaacg gccgatcgtc
      661 cccgcagcgg tcgggctgca cccggctcca cagcaccttg ccgtccgccg cgttgaccgc
      721 gtggacgccg ggccgggccg cctcgggatc aaggacgtcg ccgttgcggg tatcgttcat
      781 gtcgttgatg ggcacgtaga tggtggtgcc gtccgcggcc atgccgaagt gcacgccgcc
      841 ctgaatgctg ccccgcccca cccgcgtcca ccaaagcggc tccggatcgt cacccttcac
      901 cgcgtccagg tcgaaggcga aggcggcgcc gatcttgtgg cccccgacca gcacctgccg
      961 gccgtcagcc aggtcgacga tcatctggct ggagccgaga tcgaaatccg ggccgtcctc
     1021 cggcgggcag ttcgggttgt cggccatcat gcaggccacg ttccaggcat cgccttcggt
     1081 catctgccgc gtccagatgc gctcaccggt ggtgagatcg atggcgagca cggcatcgct
     1141 gttgccgtcc gccggggtgg aatagttctc gccggtgccc acgtagagcc ggttgcgctt
     1201 ggcgtcgatg gccggactgg cccagatggg cgcgcccgaa ggggcgtaga cgttggtgcc
     1261 cgcagacgtc ttctgcgcga cggtcggggg ctcggggatg gagtagctgc gccagcgctc
     1321 ggagccgttg gccgcgttca cggccagcac gctgccacgg aacgtgcagc actcgtaggc
     1381 ggggtcggcg gccgcgatga tctccagcga ggagacggga acgtacagga cgccctcgtg
     1441 cagcgcaggg gtacccgaca gcgtggctcc ggggtgctcg tccgccttgc gtttccagac
     1501 cggctcgccc gtgagcgcat ccaccgcgtg aacccacgcg aggatgtcgc cgaagaaggc
     1561 catgggccga ccgtgaatct ccgcgacgac gatccccgtt cggacctcgg ccgccgcctg
     1621 gtagtgccag cgcacgcagc ccgtttccag atccagggcg taaacccggc cgtcctggct
     1681 gccgacgaag acggcgccgt agccgattgc cggctgggaa cgcgcgcgca gcgcgtcggg
     1741 aaagacgaag gcccaggcga gctccagatc gggcacgtcg gccgcgctca ggccggcgac
     1801 gtcaccggga acgaagcgac gcgtgtcgtg cccccagcca acccttggcg ccggacgatc
     1861 acggtcgaag gcggcgcgag gcgcttcaca gtgggcgtcc gcaagatcgt cgacagcacc
     1921 cgccacgtag cgctgacggg tcaggtactc gacgatgtgg acgcgctcgt cggccgtcag
     1981 atcagcggcc tgggcagcca tgatcccgtc gttcatcgct ccgagcagcg tgcgcgcgga
     2041 catcatctcc agccaggtga acaggggcgc cttgggcacg ctgccgtcgt gacaggccgc
     2101 gcagttgtcg gcgtaaaggg ccgcgcccgg cagcgcctcg cggtcaccca gatcgccgag
     2161 ccccacctgg aactcgtcgg tagacgccag aacggccccc tcgccggctt cgtccgaggc
     2221 acccccggtt tcagtcggcg ccgccgcggt cggaccctcg atgggcgcct catcccgggc
     2281 atcctccggc gccgagcacc cggaagcacc ccagatcgtc agcagccctg caatgaaaaa
     2341 ggcaaacccg cgcacccgct cccccccatc cagatcagtt ggcagatgtg tgagcctagc
     2401 catttgccgg cccaaccgca aagcgacaaa atgggccggg ccaatcgggg agaaatcatc
     2461 atgtcggaag tgcgcacagc catctgcagg ttctgccact cgttctgcgg cgtgaacgtc
     2521 acgctggatg acggacggat catcaaggtg ctcggcgaca tcgagaaccc catgtatcac
     2581 ggct
//
LOCUS       DBNHKV010000041         2662 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_78122, whole genome shotgun sequence.
ACCESSION   DBNHKV010000041 DBNHKV010000000
VERSION     DBNHKV010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_78122"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..>2662)
                     /locus_tag="AC16PP_01170"
     CDS             complement(138..>2662)
                     /locus_tag="AC16PP_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6480260.1"
                     /translation="ELNHERLVRLGLPVDAVFGSIAQENSVVEAGSVSYGQRRIRIAP
                     ELAFDSVAAVGDMRVGRPGSVEIIRLGDIADISRGPVEVPLDIIRHNGRRVFTVGVSV
                     VDGRNVVEVGEAVDARLEQMLGDLPLGVEVIPIYRQHVVVSQAIDEFGRNLMLSVVTV
                     VLALCVFMGWRAGTVVGSVLLLTVMGTICVMSFLGIELQRISLGALMIAMGMLVDNAI
                     VVAEGMVIGVRRGFTPKEAAALSVRRTMFPLLGATVIGILAFAPIGLSPDKSGYYLRS
                     LFEVVAISLLLSWLLAITVVPLLGNYLLVGRETARPEELYSGWGYAPYRALLGFGLRR
                     AWLAVLVLLAVTFGCIWGFQYVKQGFFPGTNAPLFYVDYYLPQGTDIHATSGGIENVE
                     RILRTYPEVTDISAFIGKGPSRFTSIVRPEQPNPAYAHLVVRITDVNLMDAVIRRARQ
                     AVAEQIFDADIQIRRGEFTSSGTSKIEARFSGPDPDVLRHLAEQALEVYLAHDLVERK
                     TDWRQPELQIVPRFDEDRARVAGVSRTDVSRALALATLGVEIGLFRDGNKLLPIIARA
                     PHEERLDVGALSDRLVWSPAQQAHIPMRQIVSSFELVAENNTIYRRDRERTIQARANP
                     PPGHNPTMVFNSIRPGVEAIELPPGYILEWGGEHESSEDAKRLLSSKIPLTFGLMLFI
                     SILMFGKLRQPLVIWLTVPMTVCGVVVSLLVTDLTFTFTAFLGFLSLSGMLIKNCIVL
                     VDEIDKRMLEGAHSLTTIVEASVSRLRPVMLAAGTTIAGMSPLLADAFFREMAVCIMG
                     GLTFATLLTLVAVPVFYRIAFRRQLSSAQQASPGVLAGQAGG"
BASE COUNT          533 a          861 c          834 g          434 t
ORIGIN      
        1 ggaccatgtt gtccggatcc agatctttga cggcttcggc cattgcgttt ccccaagaag
       61 agtaagcgat cgggcgatgc gcatcgccct cgcgctacag attcgccgtg ctcaccacga
      121 gtgtcaaggc gctcccgtca gcctcctgcc tggccggcca ggacacccgg agaggcttgc
      181 tgcgcggagg aaagctgacg cctgaaggcg atgcggtaga acaccggcac ggccaccaac
      241 gtcaacaacg tagcaaacgt aagccccccc atgatgcaga ccgccatctc ccggaagaag
      301 gcgtcggcca gcaacggcga catgcccgct atagtggtcc cggcggcgag catcaccggc
      361 cgcagccggc tcacgctggc ctcgacgatg gtcgtcaggc tatgggcgcc ctccagcatc
      421 cgcttgtcga tctcgtccac gaggacgatg cagttcttga tcagcatgcc ggacagactc
      481 agaaaaccga ggaacgccgt gaacgtgaag gtgagatcgg taaccagcag gctgacgacg
      541 accccgcaga cggtcatggg gacggtaagc cagatcacca ggggctgtcg aagcttgccg
      601 aacatgagaa tgctgatgaa cagcatgaga ccgaaggtga gcggaatctt gctggacagc
      661 agtcgcttcg cgtcctcgct cgattcgtgc tcaccgcccc actccaggat atatccgggc
      721 ggcagctcga tggcttcaac gccgggccgt atggagttga acaccatggt ggggttgtgt
      781 cccggcggcg ggttcgcccg cgcctggatc gttcgctcac ggtcgcgtcg gtaaatcgtg
      841 ttgttctccg ccaccagctc gaagctggag acgatctggc gcatgggtat gtgggcttgc
      901 tgcgcggggc tccacacgag acggtccgac agcgctccaa cgtccagcct ctcttcgtgg
      961 ggcgcccggg caatgatggg cagcagcttg tttccgtccc ggaagaggcc gatctcgacg
     1021 cccagcgtgg ccagcgcgag cgcgcgggaa acgtccgtcc gggagacgcc cgccacccgc
     1081 gcgcgatcct cgtcgaagcg cggaacgatc tgcagctccg gttgccgcca gtccgtcttc
     1141 cgttcaacca ggtcgtgggc gaggtatacc tccagcgcct gctctgccaa atgcctgagc
     1201 acgtccggat ccgggccgga gaaccgcgct tcgatcttcg aggtcccgct ggaggtgaac
     1261 tcgccgcgcc gtatctggat gtcggcatcg aaaatctgtt ctgccaccgc ctggcgggca
     1321 cgtcgaatca ccgcgtccat caagttgacg tcggtgatcc gcaccaccag atgagcgtac
     1381 gccggatttg gttgctcagg gcgcacaatc gacgtaaagc gggatggtcc cttacctata
     1441 aaagctgata tatcagtaac ttctggatat gttctaagta tcctttcgac gttttcaata
     1501 ccgccggaag tggcatgaat gtccgttccc tgaggcaggt agtagtccac gtagaacagg
     1561 ggcgcgttgg tgccggggaa gaagccctgc ttgacgtact ggaatcccca gatgcagcca
     1621 aaggtcaccg ccaggagtac cagcaccgcc aaccaggcac ggcgcaggcc gaagccgaga
     1681 agggccctgt acggcgcgta cccccagccc gagtagagct cctccggccg agcagtttcc
     1741 cggccgacca ggaggtagtt gcccagcaga ggcaccacgg ttatcgccag cagccagctc
     1801 agcagcagcg agatggcaac cacctcgaac agggagcgca ggtagtagcc ggacttgtcg
     1861 ggcgagagcc cgatgggagc gaaggcgagt atgccgatga cggtggcgcc gagcagagga
     1921 aacatggttc gacggaccga cagcgcagcg gcttccttgg gagtaaagcc ccgacggaca
     1981 ccgatgacca tgccctcggc gaccacgatg gcgttgtcca ccagcatccc catggcgatc
     2041 atgagcgcgc ccagggagat gcgctgcagc tcgatgccga gaaacgacat gacgcagatg
     2101 gtgcccatga ccgtgagcag cagcacgcta cccaccaccg ttcccgcgcg ccatcccatg
     2161 aagacgcaga gcgcgagcac caccgtgacg accgacagca tcaggttgcg gccaaactcg
     2221 tcgatggctt ggctgaccac cacgtgctgg cggtatatgg gaatcacctc caccccgagg
     2281 ggaagatcgc ccagcatctg ctccagccgg gcgtccaccg cttcgcccac ctcgaccacg
     2341 ttgcgcccgt ccaccacgga gacgccgacg gtgaataccc ggcggccgtt gtggcggatg
     2401 atgtccagtg gaacctctac cggtccccgg gaaatgtctg cgatgtcgcc gagccggatg
     2461 atctccacgg agcccggccg gcccacccgc atgtcgccga cggcggccac ggaatcgaag
     2521 gcgagttccg gcgcgatgcg gattcggcgt tggccgtagg aaacggatcc ggcttccacc
     2581 acgctgttct cctgggcgat gctgccgaac accgcatcga ccggaaggcc gagccggacc
     2641 aggcgctcgt gattcagctc ca
//
LOCUS       DBNHKV010000042         2845 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_573655, whole genome shotgun sequence.
ACCESSION   DBNHKV010000042 DBNHKV010000000
VERSION     DBNHKV010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_573655"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="AC16PP_01175"
     CDS             complement(<1..195)
                     /locus_tag="AC16PP_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HIR6480261.1"
                     /translation="MAKRRKPMSEINVVPYIDVMLVLLVIFMATAPLLTQGVIADLPK
                     APSEPITDRQDDPLVVSIRED"
     gene            complement(206..889)
                     /gene="tolQ"
                     /locus_tag="AC16PP_01180"
     CDS             complement(206..889)
                     /gene="tolQ"
                     /locus_tag="AC16PP_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HIR6480262.1"
                     /translation="MPEQLSIFQLIANASLLVQVVMLLLALASVASWVMIFQRWFFLR
                     RVLASIADFEEHFWSGIDLRALYNELEAQDDLTGIESIFVAGFKEYTRLSEQSGADAD
                     SIMQGAQRAMRVALNREEENLETHLAFLATVGSTSPYVGLFGTVWGIMNSFRSLASMS
                     QATLASVAPGISEALVATAMGLFAAIPAVIGYNRFSARVEVMMSRYEAFSDELTGILH
                     RAAHARGGR"
     gene            complement(901..1950)
                     /gene="ruvB"
                     /locus_tag="AC16PP_01185"
     CDS             complement(901..1950)
                     /gene="ruvB"
                     /locus_tag="AC16PP_01185"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR6480263.1"
                     /translation="MSVEPDRLVSPREDGREGGFDRALRPERLDDYVGQCAVREQMEI
                     FIQAARARDEALDHTLIFGPPGLGKTTLANIIANEMGVAIKSTSGPVLERPAELAAML
                     TNLEEGDVLFVDEIHRLSPVVEEILYPALEDFQLDILIGEGPAARSLKLELPRFTLVG
                     ATTRAGLLTSPLRDRFGIVQRLEFYTVEELTQIVSRSAAKLGLEIHPEGATEVARRSR
                     GTPRIANRLLRRVRDWAEVKADGVINRKIADAALNMLKVDAQGFDSMDRRLLSAIIEK
                     FSGGPVGLDSLAAAISEERDTIEDVLEPFLIQSGYLMRTPRGRVATEQAYLHFGLSVP
                     EASEPSGGQPRLDLR"
     gene            complement(1947..2555)
                     /gene="ruvA"
                     /locus_tag="AC16PP_01190"
     CDS             complement(1947..2555)
                     /gene="ruvA"
                     /locus_tag="AC16PP_01190"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR6480264.1"
                     /translation="MIGRIQGQLVEVAENVILVNAGGICYEVELTSTALGRLPGADAL
                     VDLFTHFLVREDAQLLYGFASRDERDLFRALIRITGVGPKLALALISSIPLADLARAV
                     QTNDVALLTRVPGVGRKTAERLLVELKDRLADIVVVAEAGIPARGGKSAEEAEEVLVA
                     LGYKPQEAQRVVSDVLAEGSASTEEIVRAALRHIAMRKEVSG"
     gene            complement(2552..>2845)
                     /gene="ruvC"
                     /locus_tag="AC16PP_01195"
     CDS             complement(2552..>2845)
                     /gene="ruvC"
                     /locus_tag="AC16PP_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003145571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR6480265.1"
                     /translation="GPEVLAIEQVFMARNPDSALKLGQARGVAIAAAVQCGLPVHEYA
                     ARTVKQAVVGSGRAGKEQVQHMIRAILDLPGAPAPDAADALAIAICHVNTSRP"
BASE COUNT          502 a          969 c          890 g          484 t
ORIGIN      
        1 gtcctcacgg attgagacca ctaggggatc atcctgtcgg tccgtgatgg gctcggaagg
       61 tgctttcggc aggtcggcga tgacgccctg cgtcagcaga ggcgcggtgg ccatgaagat
      121 cacgagcagc acgagcatca cgtcgatgta cggcacgacg ttgatctccg acatgggctt
      181 cctgcgcttg gccatgaacc tgccttcacc tccccccgcg ggcatgcgcc gcgcggtgga
      241 ggatgccggt caactcgtcg ctgaacgcct cgtaacggct catcatgact tccacccggg
      301 cggaaaaccg gttgtaaccg atcaccgccg gtatcgccgc gaagagcccc atggccgtcg
      361 ctaccagagc ttccgaaatg cccggcgcca ccgaggcgag cgtcgcctga ctcatgctgg
      421 ccaggcttcg gaaggaattc atgatgcccc acacggtccc gaacaggccg acgtacgggc
      481 tcgtcgatcc caccgtagcg agaaaggcca gatgggtttc gaggttttcc tcttcccggt
      541 tcaaggccac ccgcatggcc cgctgggctc cctgcatgat ggaatccgca tccgcaccgg
      601 actgctccga cagccgggtg tactccttga agccggccac gaagatggat tcgatacccg
      661 tgaggtcgtc ttgggcttcc agctcgttgt acaacgcccg caggtcgatc cccgaccaga
      721 agtgctcctc gaagtcggcg atggaagcca gcacccggcg caggaagaac catctctgaa
      781 aaatcatgac ccacgaggcg acggacgcga gcgcgagcag cagcatgacc acctgaacca
      841 gcaggctggc gttggcgatc agctgaaata tggacaactg ctcaggcacg atcgatcaac
      901 tcacctcaga tcgaggcgcg gctggccacc ggagggctcc gaggcttccg gcacgctcag
      961 gccgaagtgc aggtacgcct gctccgtggc caccctgccg cggggggttc gcatgaggta
     1021 accgctctgg atcagaaacg gctcgaggac atcctcgatg gtatcgcgct cttcgctgat
     1081 ggcggccgcc agggagtcga ggcccaccgg cccgccggag aatttctcga tgatggccga
     1141 gagcaaccgt cggtccatgc tgtcgaatcc ctgagcgtcc actttcaaca tgttcagagc
     1201 cgcgtccgcg atcttccggt tgatcacccc gtccgccttg acctccgccc agtcgcgcac
     1261 ccggcggagc agccggttcg cgatgcgggg cgttccacga gatcttcgcg ccacctcggt
     1321 cgcgccctcc ggatgaatct ccaacccgag cttcgccgcg gatcgcgaaa cgatctgcgt
     1381 gagttcttcg accgtgtaaa actcgagtcg ttgcacgatg ccgaagcgat ctcgaagcgg
     1441 cgacgtcagc agaccggcgc gggtggtcgc gccgaccagc gtgaatcgcg gcagctcgag
     1501 cttgagcgac cgggccgccg ggccttcgcc gatgaggatg tcgagctgaa aatcctccag
     1561 cgccggataa aggatttcct ccaccaccgg gctgaggcgg tgaatctcgt cgacgaagag
     1621 cacgtctccc tcctcgagat tggtcagcat ggcggcaagc tctgccggac gctcgagcac
     1681 cggcccggag gtggacttga tggccacgcc catctcgttg gcgatgatgt ttgccaacgt
     1741 ggtcttgccg aggccgggcg gcccgaagat gagcgtgtga tccagcgctt cgtctcgggc
     1801 gcgggctgct tggatgaaga tctccatctg ctccctgacc gcgcactgcc cgacgtagtc
     1861 gtcgagccgc tcgggccgca gcgcacgatc gaacccgccc tcacggccat cttcccgggg
     1921 gctgaccaac cgatccggct cgacgctcac ccgctcacct ccttgcgcat ggcaatgtgc
     1981 ctcagggcgg cacgcacgat ctcttccgtg ctggcggacc cttcggccag cacgtcgctc
     2041 acgacgcgct gcgcctcctg gggtttgtag cccagcgcca ccaggacctc ctcggcttcc
     2101 tcagccgact tgcctcctcg ggccgggatt ccagcctcag cgaccacgac gatgtcggcg
     2161 agccggtcct tgagttccac cagaagccgc tcggccgttt tcctgcccac gccgggaacc
     2221 cgggtcagca gggcgacgtc attggtctgc acggcgcgcg ccagatcggc cagcggaatg
     2281 ctcgatatca gggccaacgc cagcttgggg cccacgccgg taatccggat cagggccctg
     2341 aagaggtcac gctcgtcccg gctggcaaag ccgtagagca gctgagcgtc ctcgcgaacc
     2401 agaaagtgcg tgaagaggtc gacgagggcg tccgccccgg gcaaccggcc cagcgcggtg
     2461 ctggtcaact ccacctcgta gcagatacca ccggcgttga cgaggatcac gttttcggcg
     2521 acctcgacga gctgaccttg gattcgcccg atcatgggcg ggaagtattg acgtgacaaa
     2581 tggcaatcgc aagcgcatcc gccgcgtcgg gtgccggcgc cccgggaagg tcgaggatcg
     2641 ccctgatcat gtgctggacc tgctcctttc ccgcgcgccc cgagccgacc accgcctgct
     2701 tgaccgtgcg ggccgcgtac tcgtggacgg gcaacccgca ttggacggcg gcggctatcg
     2761 ccacgccgcg ggcctgtccc agcttcagcg ccgagtcggg attccgggcc atgaatacct
     2821 gctcgatggc cagcacctcg gggcc
//
LOCUS       DBNHKV010000043         4040 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_573709, whole genome shotgun sequence.
ACCESSION   DBNHKV010000043 DBNHKV010000000
VERSION     DBNHKV010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_573709"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="AC16PP_01200"
     CDS             complement(<1..241)
                     /locus_tag="AC16PP_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480266.1"
                     /translation="MIDAFNYFVFVLVPIFLLGFQAVLLAVLFFLKARKWLSVYAFAS
                     GGFVAGLGFGYLFATLSGSTQTLLVVCLSVGAFGIL"
     gene            complement(290..634)
                     /locus_tag="AC16PP_01205"
     CDS             complement(290..634)
                     /locus_tag="AC16PP_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480267.1"
                     /translation="MNLDLTLKAAAILLLLISGMEYLVSSVIHLGPVLFANVMFVTSI
                     FSRTQPRRIAVIALAFAVVIPIGAFRAYVRDDSALWVALLNLAIFCYVAFVAVQTLTA
                     PSGRNIGNGQGS"
     gene            complement(750..1289)
                     /locus_tag="AC16PP_01210"
     CDS             complement(750..1289)
                     /locus_tag="AC16PP_01210"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR6480268.1"
                     /translation="MRQISSIPFDIFSVRFKARLALLALGPLLGSAPAALADLKIDRL
                     VIEKSQRVLILEHDGAPVRSYSIALGGAPRGHKQREGDQRTPEGQYTIDARNEDSAFH
                     RSLRISYPNEADRRRAAMEGVNPGGQIMIHGIGEADPEMRKLHPYIDWTAGCVAVTDS
                     EIEEIWALVEIGTPIVIRP"
     gene            complement(1371..1844)
                     /locus_tag="AC16PP_01215"
     CDS             complement(1371..1844)
                     /locus_tag="AC16PP_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480269.1"
                     /translation="MPQHPREPDIASWHRYFAIECNNVAWDLAGRARTAPEDEQMLQA
                     AHAAAFHWDQVGEELNRMRATMLLAKVHALLGKNPSALRYAEEMQTFFLDRDTPDWEI
                     AFAHAIYAHAAYVAGDYTAHARAYRDALQALEVIADPEDHMLVRITFDQVPPPEP"
     gene            complement(1884..3563)
                     /gene="ilvD"
                     /locus_tag="AC16PP_01220"
     CDS             complement(1884..3563)
                     /gene="ilvD"
                     /locus_tag="AC16PP_01220"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017338119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR6480270.1"
                     /translation="MTDQKRPYSSIVVDGVQQAPSRAMLYPVGFTEADFAKPQIGIAS
                     TWSMVTPCNMHIDALAEAAALGADEAGGKSVVFNTITVSDGISMGTPGMRYSLVSREV
                     IADSIETVVGAQGFDGFVAIGGCDKNMPACAMAMARLNRPSVFVYGGTIQPGAERRDI
                     VSVFEAVGAHAAGRISDIELKEVEATAIPGPGSCGGMYTANTMASAIEAMGLSLANSS
                     AQEAISNAKRQDSYQAGAAVFNLIERGIKPSDILCREAFENAITVAIALEGSTNAVLH
                     LLAIAHAAGVPLELDDFSRVGERVPVLADMRPAGQYAMSELIAIGGIQPLMKTLLEEG
                     LLHGDCLTVTGKTLAENLADVAPYPDGQNVIRPMSDPIKKDSHLVVLYGNLAPTGAVA
                     KITGHEGLSFTGSAKCFHGEEAAMAAIMDGAINKGDVVVVRYEGPKGGPGMREMLSPT
                     SAINGRGLSQDVALITDGRFSGGSHGFVIGHITPEAYEGGPIALIEDGDLITVDAEKR
                     EINVQLSDADLAARRARWQKPAPYATKGILAKFARLVSSASEGAVTDKNLD"
     gene            complement(3596..>4040)
                     /locus_tag="AC16PP_01225"
     CDS             complement(3596..>4040)
                     /locus_tag="AC16PP_01225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6480271.1"
                     /translation="RQARLEDVAGIVELIRPMEEAGVLVRRPRDRLEQEIDRFFVAEV
                     DGIVMGCCALYPFEDCGELACVAVHESHRSKPGETRLGVALLRATEAAAGAMGLRKLF
                     VLTTQTRDWFVDNGFEDSTLDALPAPKQAMYNYQRNSKVMIKQLR"
BASE COUNT          709 a         1284 c         1314 g          733 t
ORIGIN      
        1 acaagattcc aaacgcgcct acggacaggc acacgaccag cagcgtctgc gtcgatccgc
       61 tcagcgtggc gaagaggtag ccgaacccca ggccggcgac gaagccgccg ctggcgaacg
      121 cgtaaaccga gagccacttt cttgccttga gaaagaagag gacggcgagc agcacggcct
      181 ggaaacccag cagaaatatg ggtaccagaa cgaatacgaa atagttgaac gcgtcgatca
      241 cgtccggtct ccagtctttg cttgtcggaa ggctacacgg gccgggcccc tacgaacctt
      301 gcccgttccc gatgttcctg cccgaaggcg ccgtcagcgt ttgcacggcg acgaaggcaa
      361 cgtaacagaa aatcgccagg ttgagcaggg cgacccacag ggcggagtcg tccctgacgt
      421 aggcccggaa cgcgccgata ggaatgacga cggcaaacgc gagagcgatg acggcgattc
      481 gccgaggttg ggttcggctg aaaatgctgg tgacgaacat cacgttggca aacagcacgg
      541 gaccgaggtg gatgaccgac gagaccaggt actccatgcc ggagatcagc agcagcagta
      601 tggcggcggc tttcagggtg agatcgagat tcaagatgaa ggatcctcga ggccgtcgat
      661 cgggcgccgt ccgggttgag gggcgagctc ggctgatgac gtggcggcgc ggttcatctg
      721 cagtgcgtcg agtccggcgg ggcgcgtcgt cagggccgaa tgacgatggg ggtaccgatc
      781 tcgaccaggg cccagatctc ctctatctcg ctgtcggtca ccgcgacgca gccggcggtc
      841 cagtcgatgt aggggtgcag tttgcgcatt tccggatcgg cctcgccgat gccgtggatc
      901 atgatctgac caccgggatt gaccccctcc atcgccgctc gccgccggtc ggcctcgttc
      961 gggtaggaga tccgcagaga gcggtggaat gcgctgtcct cgttgcgggc atcgatggtg
     1021 tattgcccct ccggcgtgcg ctggtcgccc tcgcgctgct tgtgcccccg cggcgccccg
     1081 cccagcgcga tcgagtagga tcgcacgggt gccccgtcgt gctcgaggat cagcactcgc
     1141 tgagacttct cgatcaccag ccggtcaatc ttgagatccg caagcgctgc cggtgccgac
     1201 ccgagcagcg ggcccagcgc cagaagcgcc agtcgggctt taaagcgaac ggaaaagatg
     1261 tcgaaaggga tcgaggaaat ttgcctcatg cccgtaccat gaccgcgtga cctctcgagc
     1321 ccgaagatag cacgtgaatt ctcacctgca acggtcagtt tgtatccggg ttacggctcg
     1381 ggtggcggca cctgatcgaa ggttatccga accagcatgt ggtcttcagg gtcggcgatg
     1441 acctcgagcg cctgcaaggc atcccgatag gctcgcgcgt gggcagtgta atccccggct
     1501 acgtaagcag cgtgggcgta gatcgcgtgg gcgaaggcga tctcccaatc gggcgtgtcc
     1561 cggtccagaa agaaggtctg catttcctcg gcgtaccgca gcgcggaggg gttcttgccc
     1621 agaagcgcgt gcaccttggc gagcagcatt gtggcccgca tcctgttgag ctcctcgccg
     1681 acttgatccc agtgaaaggc cgcggcatgc gcggcttgca gcatctgctc atcctcaggc
     1741 gccgtgcggg cgcggccggc caggtcccaa gccacgttgt tgcactcgat ggcaaagtat
     1801 cgatgccatg atgcgatgtc gggctcccgc ggatgttgcg gcacgtctgc ttatccccgt
     1861 ttcacggcat gctgccgatg ggctcaatcc aggttcttgt cggtaacggc gccttcactc
     1921 gccgagctga ccagccgggc gaacttcgcc agaatgcctt tcgtggcgta cggcgccggt
     1981 ttctgccagc gggcgcggcg cgcggccagg tccgcgtcgc tcaactggac gttgatctca
     2041 cgcttctcgg catcgacggt gatcagatcg ccgtcctcga tcagcgcgat gggccctccc
     2101 tcgtaagcct cgggcgtgat gtgaccgatg acgaagccgt gggagccgcc ggagaaacgc
     2161 ccgtcggtga tcagcgccac gtcctgacta agaccgcggc cattgattgc gctggtgggc
     2221 gagagcatct cgcgcatccc cgggccgccc ttcggccctt cgtagcgtac gaccaccacg
     2281 tcgcccttgt tgatggcccc gtccatgatg gcggccatgg cggcctcctc gccgtgaaag
     2341 cacttcgcgc tgcccgtaaa ggacaacccc tcgtgtccgg tgatcttcgc cacggccccg
     2401 gtgggggcca ggttgccgta gagaacgacc aggtgggaat ccttcttgat cgggtcgctc
     2461 atgggacgga tgacgttctg gccatccgga tacggggcca cgtcagcgag gttctcggcg
     2521 agggtctttc cggtgaccgt gaggcagtcg ccgtgcagca ggccctcttc gagcagcgtt
     2581 ttcatgagcg gctggatgcc gccgatggca atgagctcac tcatggcgta ctggccggcc
     2641 ggacgcatgt cggccagcac gggaaccctc tcgccgacgc gggagaaatc gtccagctcc
     2701 aggggcacgc cggctgcgtg ggcgatggcg agaaggtgca gaacggcgtt ggtcgatcct
     2761 tccagggcga tggcgacggt gatggcgttc tcgaacgcct cgcggcagag aatgtcgctg
     2821 ggcttgatcc cgcgctcgat caggttgaac accgccgcgc cagcctggta gctgtcctgg
     2881 cgcttcgcat tcgagatcgc ttcctgggct gagctgttcg ccagcgacaa acccatagcc
     2941 tcgatggcgg atgccatggt gttcgccgtg tacatgccgc cgcaggaacc gggccccggg
     3001 atcgccgtgg cctcgacttc cttgagctca atatcggaga tccgtcccgc cgcgtgagcg
     3061 cccacggcct cgaataccga gacgatgtcg cgtctttcgg cgccgggctg gatggtcccg
     3121 ccgtagacga acacgctcgg acggttcagg cgggccatgg ccatcgcgca ggcgggcatg
     3181 ttcttgtcac aaccgccgat ggcgacgaac ccgtcgaatc cctgggcgcc gacgacggtc
     3241 tcgatggagt cggcgatcac ctcccgcgaa accagcgagt aacgcatacc cggcgtgccc
     3301 atgctgatgc cgtcggaaac ggtgatcgtg ttgaacacca cgctctttcc gccggcttcg
     3361 tcggcgccga gcgctgccgc ctcggccagg gcgtcgatgt gcatgttgca cggagtgacc
     3421 atgctccacg tggaggcgat cccgatctgt ggctttgcga agtccgcctc cgtgaaaccc
     3481 acggggtaga gcatcgcgcg gctcggagcc tgctgcacgc cgtccacgac gatggacgag
     3541 tagggtcttt tctggtcagt catggagttc ccctggggcc cggcctcact gccggttacc
     3601 tgagttgctt gatcatcacc ttcgagttgc gctggtagtt atacatagcc tgcttgggcg
     3661 ctggaagcgc gtcgagagtg gaatcctcga agccgttgtc cacgaaccag tctcgggtct
     3721 gggtggtcag cacgaagagc ttcctcaggc ccatcgcgcc cgctgcggct tccgtcgccc
     3781 ttaagagcgc aaccccgagg cgggtctctc caggcttgct gcgatgggat tcgtggacgg
     3841 ccacgcacgc gagctccccg cagtcctcga agggatacag agcgcagcaa cccatcacga
     3901 tgccgtccac ctcggcaacg aagaaacgat cgatctcctg ctccagcctg tcacgcggac
     3961 gccttaccag cacgccggcc tcctccatgg gcctgattag ctcgacgatg ccggccacgt
     4021 cttcgagccg cgcctgccgc
//
LOCUS       DBNHKV010000044        19992 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_928516, whole genome shotgun sequence.
ACCESSION   DBNHKV010000044 DBNHKV010000000
VERSION     DBNHKV010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 19992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19992
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_928516"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="AC16PP_01230"
     CDS             <1..342
                     /locus_tag="AC16PP_01230"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR6480272.1"
                     /translation="TAFDIDQAGNLSNRRDWAKLPDGAVPDGICLDSDGGIWTASPAT
                     NECLRQIEGGTVTHRVPVNQGAFACMLGGDDGRSLFILTAGSSAPDACRAARSGRVEL
                     VTAPFPHAGWP"
     gene            381..1535
                     /locus_tag="AC16PP_01235"
     CDS             381..1535
                     /locus_tag="AC16PP_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6480273.1"
                     /translation="MYENYESPWLNDELRLLQDAAAKFFQQEFVPLNEKWINQGKVDR
                     EAWYKAGEAGLLCAEMPVEYGGGGGDYRHETVIMEEQLATGVTGFGNQVHSSIVAPYI
                     LHYGSEDQKQRWLPKMATGELVGAIAMTEPNTGSDLQAVRTSAIRDGDEYVINGSKTY
                     ISNGQHADLVIVVAKTDPAQGAKGISLIVVETGKSPGFERGRNLKKLGQKAADTSELF
                     FADCRVPVTNRLGEEEGRGFVQLMQQLPQERLNIGQAAVVAMERAIGITLDFVRERRA
                     FGQRILDFQNTRFKLSECKTEALIARTFVDQCVLKLVNGELDAVTASMAKYWCTQKQC
                     EIIDECLQLHGGAGYMDEYEISRMYADARVQTIYGGTNEIMKELIGRSLG"
     gene            1543..2823
                     /locus_tag="AC16PP_01240"
     CDS             1543..2823
                     /locus_tag="AC16PP_01240"
                     /inference="COORDINATES: protein motif:HMM:NF018913.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480274.1"
                     /translation="MIRYWRFYWPLALTGLAMVLAVQFQNAALARFPEAVTELAVFAL
                     AYSAYALFHASLNFVAQLSNVYARSPQGTSRSWRFATAASACIMLPLLALAHTDYGAD
                     ALGSIYGIDDGLVERVTEYLAYLAPLLLISAQRFYYNGLLVQARLTGWVTTLTVVYLT
                     SVLVGLIIGFTLGLKPVHVLVGAEGIALLIQLALSVLIKASRYAPPADPEHADLSYGE
                     LLRFYLPVSTTAVMFALSRPILYAFVARTPNGVLAIAAMRVAFDFATIFQQAANQFRH
                     FFVTFGWNDLGIKRRFLTVICAGITAIMLAVALTPLSELIWGKLMGIPEPVRDIAEHV
                     LVVMCLMPALIVFRNYYHGRLMVERRTSGMAAGGILRVIAILLAAQACTMMGWLNYLS
                     ASFILILGFLVEALVALVAAQKGQGLRANATANM"
     gene            complement(2778..3137)
                     /locus_tag="AC16PP_01245"
     CDS             complement(2778..3137)
                     /locus_tag="AC16PP_01245"
                     /inference="COORDINATES: protein motif:HMM:NF016161.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HIR6480275.1"
                     /translation="MLKSIHLILAYLTVLGFVVRGIWSLVDSPLKNQKWVRISPHVID
                     TALLVLGVALAVQLSVSPLEGWLAAKLAGLLAYIGFGVLTMRASSTALKLAGFAGALA
                     SVGYIFAVAFARSPWPF"
     gene            3225..4688
                     /locus_tag="AC16PP_01250"
     CDS             3225..4688
                     /locus_tag="AC16PP_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR6480276.1"
                     /translation="MNIDQAIDQAMRPLADAVSGFIFFSVPVLGTQIPLIVVWLILGG
                     LFFTVYLRFVNIRGFAHAIGLVSGRYHNDQHPGEISQFQALTTAVSGTVGIGNIAGVA
                     VAISMGGPGATLWLIIAGLLGMSTKFAECTLGLKFRRHHEDGSVSGGPMYYLERGLAE
                     RNWPRVGRGLGLFYAASMVIGCLGIGNMFQSNQAAAIFIDVSGGDASYFQDKAWLLGL
                     IMAGAVGLVIIGGIRSIARTTSRLVPGMALLYVISALAIIAINAAELPAAFAAIWAGA
                     FSPEGIAGGLIGVMIIGFRRAVFSNEAGLGSAAIAHSTAKTERPVSEGYVALLEPFID
                     TVVICTMTALVIITTVYEPGLTSGNVSGIELTTRAFASTLSWSPIPLSIAAILFAFST
                     MIAWSYYGLKAFTYLVGHHRVADLGFKIFFLVFVVLGSSVQLGALVDLSDALVFVVAI
                     PNLLGLYLLAPTVRAELRAYESNMPVPNGAAPAPDST"
     gene            4743..5612
                     /locus_tag="AC16PP_01255"
     CDS             4743..5612
                     /locus_tag="AC16PP_01255"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6480277.1"
                     /translation="MEGPTSHVYFSQRLRLHYVDWGNPDAPPMLMIHGGRDHCRNWDW
                     VARSLRDRYHIIAPDLRGHGDSQWMLGGSYNQIDYVYDIAQLLTQKDMTPVTIIGHSL
                     GGSIALTYSGLFPETVKKLVAIEGMGPPPEFIKERLAVPVADRLHQWISDLRELSGRI
                     PRRYATLEEAYERMQTENPHLSEEQARHLTIHGSNQNEDGTYSWKFDNYVRAFPPIGI
                     DFEEQYKLHGRITCPTLLIRGTESWASDPSEDGRAARFQNATVRNIERAGHWVHHDQL
                     DEFLEIVDGFLAD"
     gene            5615..6676
                     /locus_tag="AC16PP_01260"
     CDS             5615..6676
                     /locus_tag="AC16PP_01260"
                     /inference="COORDINATES: protein motif:HMM:NF013621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480278.1"
                     /translation="MSQRAVILHRSPWQGVFHVTGGGSGLLAELLTTPGASGTVLEAV
                     VPYAQAALADLLGRAPDQACSPSTARSLAMSAFDRARDLGAREPFGLGCTASLATERM
                     KRGKHRACIAVQTTTSTYSATLQLEGDRASEERDLVELIWHALSRTLELHLESAPPDT
                     FDGGLTSALEHWRALVLGEETAHATAPHDGRLLLPGSFNPLHRAHRRMLELAEHLTGL
                     EGAYELSIVNVDKPLLDYTEIEERLSQFEAPVWLTRLPTFVDKARYFPGAHFAVGVDT
                     IIRITEAGYYGSQADRDAALDELATLGTRFLVFGRILNDAFLTLSDLDLPATFRALCR
                     EVTEAEFNEPISSTEIRRR"
     gene            complement(6691..7581)
                     /locus_tag="AC16PP_01265"
     CDS             complement(6691..7581)
                     /locus_tag="AC16PP_01265"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF018533.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fpg/Nei family DNA glycosylase"
                     /protein_id="HIR6480279.1"
                     /translation="MPELPDLTVYVEHLERRLVGQPLEAIRIASPFVLRSVEPTVDEV
                     CGRRILGCHRLAKQLVMELEGEHFVVVHLMIAGRLKWQKPSAPLPKPNALAAFDVPAG
                     SLLLTEASSKKRASLRLVRGAQALGALNPGGLEVLSTDLAAFASRLRESNHTLKRALT
                     DQHVFAGIGNAYSDEILLRARLSPFKRPRDMADGEIQRLHEATKGVLGEWTDRLRAAA
                     GDDFPSKVTAFHPEMAVHGKYRQPCPVCGAPVQRIVYAENEANYCARCQTGGRLLADR
                     SLSRLLKENWPKRVEELEGG"
     gene            complement(7574..8953)
                     /locus_tag="AC16PP_01270"
     CDS             complement(7574..8953)
                     /locus_tag="AC16PP_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR6480280.1"
                     /translation="MSEGGQAGELQGRRPGIWELAFPSILSNLSFTLVGLVQTKFVGE
                     LGAQALAAVGAGQRVFFAMQAILMAVSAGTTALVARAWGAGDYVEASRVTMASLVLAG
                     AFSVVVAVIGLFFAPVIAGVFGLDPETLGMASENIRWLCVFNVAFAVNFILGAALRAS
                     GDAWSPLWISGAVNVVNVPLLYVFIFGEFGFPRMGVAGAAIAAGIAFSIGGAVLLGLW
                     VKQKFRVKHVASGWWRKARLRQLLDIGYPAAVEQGVFQLGFFIFLMLIGNFYGTEAFA
                     AYNIGANMLMVCMTVGFGFSIAGATLVGQHLGANDPDGAARSGWRSTGLAVLAMGGLG
                     GVVCFYAEELAVFFIGDEPLTVDYTVQFTYILGAMMPLLAVEFTIGGALRGAGDTRFP
                     LMATFLGLIGMRCGLAALATYLGVPVFWVYAALIGDYLLKGVLLISRFRGGRWKTAIR
                     YREPEALGA"
     gene            9055..9855
                     /locus_tag="AC16PP_01275"
     CDS             9055..9855
                     /locus_tag="AC16PP_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6480281.1"
                     /translation="MTEYTDIGVTQDGHVTTVEIQRPPNNFFDHSLIRQIADAFNAAD
                     EDPNCRAIVLAAQGRHFCAGANFGSGKDDDSGSEDFTEEGFRNTTGILYREAARLFGS
                     KKPVIGAIQGAAIGGGLGLSLVPDFRVACPEARFGANFVKLGIHQGFGISVSLPRIIG
                     QQAASMLLLTGRRIGGEEAFKLGLVDVLTDAESLRDEAMKLAQEIAENAPLAVLSVRG
                     TMRAGLAEAVIEATEHELKEQQWLRATEDAQEGIRSVAERRPGNFQGR"
     gene            9857..10729
                     /locus_tag="AC16PP_01280"
     CDS             9857..10729
                     /locus_tag="AC16PP_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6480282.1"
                     /translation="MKLGKLGVWYFLDGLPADAAAAAAQRIEELGYSALWIPEATGRN
                     PMVHASWLLANTKQLIVATGIANIYHREPGVTVSAQKALAEQSGDRFLLGIGVSHKPF
                     VEGVRGLTYGPPVASMRDYLDKMAAAPYTSVPPAEDPPTVIAALGPKMLALAAEKTAG
                     AHPYFVSPDHTAMASEVLGPDKWLCVEQKVILETDPSRARELARPAAQMYLALPNYRN
                     NWLRMGFTEADLGNGGSDRFIDATFAWGSAAQVEARIREHFDAGATHVCIQPVNPNGQ
                     FGDLHWEALEALAP"
     gene            10726..11409
                     /locus_tag="AC16PP_01285"
     CDS             10726..11409
                     /locus_tag="AC16PP_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6480283.1"
                     /translation="MSSDYTGNVVVVTGAAGALGRAVVEHFAASGATVAQLDVVAIDN
                     AHFSDTCDLTDPDSCRQAVTRIIETLGLIDVLANIAGGFTMGEAVHETSEETWDFMMG
                     LNARSVLNMARAVVPIMLQQGAGKIVNVGAGAGLAGVANMGAYSASKSVVIRLTEAMS
                     AELKERRINVNCVLPSIIDTERNRADMPDADFHRWVTPAAMAGVIGFLASPAADPIHG
                     AAVPVSGLS"
     gene            complement(11444..13186)
                     /locus_tag="AC16PP_01290"
     CDS             complement(11444..13186)
                     /locus_tag="AC16PP_01290"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6480284.1"
                     /translation="MVNRRRFVEAGALTAAGLSLWGCGADTRPRSAGAGDGQVAGPGR
                     AVVFRSGVVLPVDAGFSEQRAIAIRGNRILAVGDDDDVLAAAGKDPQVVDLDGRVLLP
                     GFIEPHMHFALMAGLGHWPDIGPFKHESTEDALAALRLIARETPADEWIVARQFDPSL
                     QTGPEMLTTRELDTVSTDRPVFVLNASGHLAYGNSRLLEFAGIDRDTPDPPGGEYFRD
                     PDGRPNGAMSQVAYQPVMMRNEALIAVLGSGFVEAGLRVGEEAAALGITTLCDQATGG
                     IAGMGDIDFYRQMFASSRMSARIRASLYSETAAEWDDNGVAFGDGDALMRIVGWKIVT
                     DGSNQGFTGRQREPYLDSDNRGMYYVAPEALNEMVLSRGRRGWPLVLHGNGDAAIDSI
                     LDAVQAAADAGVDVGKLRCRIEHASILHDGQIQRIRELGMGPSFLINHVHYWGHAMRD
                     KVFGPEKVQLLDRCRSVEDAGITWTMHSDAPVSPLGTLHKIRVAVARDLWKEPDTTLA
                     PHERVSVEAAIRAVTINAAWQCHSETEVGSLAEGKLADLVVLEDDPRKVEPKAISEIG
                     VSQTWMDGNQVFGA"
     gene            13461..14558
                     /locus_tag="AC16PP_01295"
     CDS             13461..14558
                     /locus_tag="AC16PP_01295"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024151.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6480285.1"
                     /translation="MTSYDPFLRGPHPVGVRTFHWTDKTRQRTLPVEVWYPADDEHAG
                     QDLDPDTQDHFQPMSLSPPVPQAAVRDAAPAEGEPRPLLIFSHGFGGTGRQTTHLCTH
                     WASHGYVVAAMDHVGNTTEDVMAAAAQPVDPGTLLERLKSFAADRPVDVSFVIDRMVA
                     GDAGLTVDPDRVGVSGHSFGGWTTLASAGMDPRIRAALPLAPAGGQSSQWQSSIADRL
                     ADALAMSWEREVPTLFLVAEHDVVLPLDSMHDLIERTPHPRRAVNLLNADHFHFCDRV
                     EQTHDMVRMMGQMIFGTRSEADQEAPSALAAMKPSSELCPGDHAYALLQGLGLAHMDA
                     HVRELDRAHALLAGDLASLMAERGVAVKVMA"
     gene            complement(14611..15090)
                     /locus_tag="AC16PP_01300"
     CDS             complement(14611..15090)
                     /locus_tag="AC16PP_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6480286.1"
                     /translation="MSDTETNMNNALEILRQRLDQPNPSSDWFEITQDRVNDYADVSM
                     DHQWIHVDVERAKQGPFGAPIAHGNLTIAVMGHLPRSETASQPPLEGHKLSINYGFDR
                     IRFPAPVRVGDRIRATSTPRRAEIKGGMIETMNEIVVEIEGQEKPALVAESLGRLVF"
     gene            complement(15199..16320)
                     /locus_tag="AC16PP_01305"
     CDS             complement(15199..16320)
                     /locus_tag="AC16PP_01305"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR6480287.1"
                     /translation="MNLKTRVCEVLEIEHPIVLAGMGGASCPALAAAVSNAGGLGVLG
                     AAGCGPELLRRWIRETRELTDRPFGVDTLLPASVRRANYDETEGPTPMDLLPETQAYA
                     AEFMEREGLEPVASESPRPPRTSDEPAVFTKDFFEAQMRVVIEERVPVYASGLGNPGP
                     WMDELRANGTKVMAVVGAVRHAIQVKSSGLDIIVAQGHDGGGHNSPIGTMALIPQVVD
                     AVGDTPVLGAGGIVDGRGIAAAMMLGAEGVWIGSAFLASQEANIFDFQKQAIVDATEE
                     GTSVSRSVTGKPARIIRSKWTDYWARGERDPLPMPFQGLVSQPVLAAANRDGRGDINP
                     GFAGQGIGMIKAVRPAREIFDELVYGAEQALARTSNLLR"
     gene            complement(16320..16805)
                     /locus_tag="AC16PP_01310"
     CDS             complement(16320..16805)
                     /locus_tag="AC16PP_01310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR6480288.1"
                     /translation="MSEIDQRVFRDTMGQFCTGVVIATGCREGEPAGFAAQSFSSVSL
                     DPPLVVLCPAKTSTSWPKLRDSGSFCINILGEDQKAVCDVFAQSGVEKFASLDWSPGA
                     TGSPVLADVIAYVECDLHAEHDAGDHTLAVGLVREVSIVNPDKPPLLFFRGGYGQFEG
                     L"
     gene            16928..18121
                     /locus_tag="AC16PP_01315"
     CDS             16928..18121
                     /locus_tag="AC16PP_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6480289.1"
                     /translation="MTDLNEFRKEVRNWLEENCPPSMRTSTPEGETVWGGRREKFANP
                     DSRIWLDRMAERGWTAPTWPREYGGGGLTADEDKVLQQELRSINARPALQSFGIWMLG
                     PALLEFASEEQKLEHIPQIVRGEIRWCQGYSEPGYGSDLAGLQTKAEDQGDHFLVNGS
                     KIWTSYADVADWIFCLVRTDNDAPKHEGISFLLFDMDSEGVTTSPIELISGASPFCQT
                     FFDNVRVPKANLVGGLNKGWSIAKRLLQHERQMVAGIGGMSALGGTGRRLEDVAKAYV
                     GEAQGRIGDPAVRSSVIEHRMNDRAFQLTLERSGDEAKAGQADGNLASMFKYYGTEQN
                     KRKYELMLQAMGTQSMGWEGEAFSAKELGTTRQWLRSKANSIEGGTSEVQLNVIAKRV
                     LGLPD"
     gene            18164..18757
                     /locus_tag="AC16PP_01320"
     CDS             18164..18757
                     /locus_tag="AC16PP_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009043301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR6480290.1"
                     /translation="METIIVLVIIAALAFWVIAIYNRLVTLKNRFKNAFSQIEVQLKR
                     RYDLIPNLVETAKAYMSHERETLEAVIAARNQALAGLQTAAQDPGNADAIRQLASAEG
                     ALGGALGRLNVVMEAYPDLKANQNMMQVSEELTSTENRVAFARQAYNDQVTDYNTYKQ
                     SFPAMLLAGPFGHAGDAALLEYEDSEAMQEAPKVSFS"
     gene            18774..>19992
                     /locus_tag="AC16PP_01325"
     CDS             18774..>19992
                     /locus_tag="AC16PP_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR6480291.1"
                     /translation="MNFFRAQDDAKRKTWRLALLFAAAVGSLIVLTNLLLMGVYRWTT
                     DVGVATSPDAIAAFTNVPTEQWLIVTAAVVAVVGIASLYKYLVIRDGGKTIAEALGGR
                     RINANTTDPAERRLLNVVEEMAIASGTAVPPVYLIPEHSINAFAAGFTPDDAVIGINQ
                     GTLDQLNRDELQGVVGHEFSHILNGDSRINLRLIAMLHGILFIGIIGYGILRGMAHGG
                     NRRSGGNAAVPVMALGLGLLIIGYSGTFFGNLIKAAVSRQREYLADAAAVQFTRNPSG
                     LANALKRIGGLPVGSTMSGSNAREISHMFFGQCSRPFFSGLMSTHPPLEDRIRAIEPR
                     WDGRLLERGQPSPTRPAGAAGFAGTGAAGIQVPGGISVGVPAESIADEVGRLDQTSLD
                     NAVGIIASIDPKLR"
BASE COUNT         3572 a         6779 c         6372 g         3269 t
ORIGIN      
        1 accgctttcg acatcgacca ggccggcaac ctgtcgaacc gcagagactg ggccaagctc
       61 cccgacggcg ccgtgcctga cggcatctgc ctggattccg acgggggaat ctggactgcc
      121 tcgcccgcga ccaacgagtg cctgcgccag atcgaagggg gcaccgtcac gcaccgggtc
      181 ccggtgaacc agggcgcttt tgcgtgcatg ctcgggggag acgacggccg ctccctcttc
      241 atcctcaccg ccgggtcatc ggcgcccgac gcctgccgtg cggcccgctc cggacgcgtg
      301 gagctcgtta ccgccccgtt cccgcacgcc ggctggccgt aaaagccgcg caaccgactc
      361 acacgaggaa acaagacgtg ctgtacgaaa actacgagtc accctggctg aacgatgagc
      421 ttcgcctgct ccaggacgcc gcagcgaagt tttttcagca ggagttcgtg ccgctgaacg
      481 agaagtggat caaccagggc aaggtggacc gtgaagcctg gtacaaggcc ggcgaggcag
      541 gcctgctctg cgcggagatg cccgtcgagt acgggggcgg tggcggcgac taccgccacg
      601 agaccgtcat catggaggag cagctcgcca ccggggtgac cggcttcggc aaccaggtcc
      661 acagcagcat cgtcgccccg tacatcctgc attatggctc cgaagaccag aaacagcgct
      721 ggctgccgaa gatggccacc ggcgagctcg tcggcgccat cgccatgacc gagcccaata
      781 cgggctccga tcttcaggcc gtgcgcacgt cggcgatccg cgacggtgac gagtacgtca
      841 tcaacggctc caagacctac atttccaacg gccagcacgc cgatctggtc atcgtcgtgg
      901 caaagaccga tccggcccag ggcgcgaagg gcatatcgct gatcgtggtg gagacgggca
      961 agagtccggg tttcgagcgg ggacgcaacc tcaagaagct gggacagaag gccgccgaca
     1021 cctcggagct gttctttgcc gactgccggg ttcccgtcac gaaccgcctc ggcgaggagg
     1081 aaggacgagg cttcgtccag ctgatgcagc agctgcctca ggaacgcctg aacatcggcc
     1141 aggcagccgt ggtggccatg gaacgcgcca tcgggatcac gctcgacttc gtcagggaga
     1201 gacgcgcctt cggtcagcgg atcctggatt tccagaacac cagattcaag ctgtccgagt
     1261 gcaagacgga ggccctcatc gcccgcacct tcgtggacca gtgcgtgctc aagctcgtca
     1321 acggcgagct cgacgccgtc acggcatcca tggccaagta ctggtgcacc cagaagcagt
     1381 gcgaaatcat cgacgagtgc ctgcagctgc acggcggtgc cggctacatg gacgagtacg
     1441 agatctcgcg gatgtacgcc gatgcaagag tgcagacgat ttacggcggc accaacgaga
     1501 tcatgaagga gctcatcggc cgcagcctgg gatagttcca ggatgatccg gtactggcgc
     1561 ttctactggc ctctcgccct caccggcctg gccatggtac tggccgtgca gttccagaac
     1621 gcggcgctcg cccgtttccc ggaagccgtt accgagctgg ccgtgttcgc gctggcatac
     1681 agcgcgtacg cccttttcca cgccagcctg aacttcgtcg cgcaactctc gaacgtctac
     1741 gctcgcagcc cccagggcac ctcacgcagc tggcgattcg ccaccgctgc gagcgcgtgc
     1801 atcatgctgc cgctgctcgc gctcgctcac accgactacg gcgccgacgc gctgggctcg
     1861 atctacggca tcgacgacgg gctcgtcgag cgggtcacgg aatacctcgc ctacctcgct
     1921 cctctcctgc tgatcagcgc gcagcgcttc tactacaacg gtctgctcgt gcaggcccga
     1981 ttgaccggct gggtgaccac gctgaccgtc gtctacctca ccagcgtgct ggtcgggctg
     2041 atcatcggct tcacgctggg actcaagccc gtgcacgttc tggtcggcgc ggagggcatc
     2101 gccctgctga tccagctggc gctgtccgtg ctgatcaaag caagccgcta cgcccctccg
     2161 gcagacccgg agcacgcgga cctgtcctac ggcgagctgc ttcggtttta tctcccggtg
     2221 tcgaccacgg cggtcatgtt cgcgctgtca cggcccatcc tctacgcgtt cgtggcacgc
     2281 acgcccaacg gagtgctcgc catcgccgcc atgcgcgtag cgttcgactt cgccaccatt
     2341 tttcagcagg ccgccaacca gtttcgccac ttcttcgtga ccttcggttg gaacgacctg
     2401 ggaatcaagc gccgcttcct caccgtgatc tgcgcgggta tcaccgcgat catgctggcc
     2461 gtggcgctca cacccctgtc cgagctcatc tggggcaagc tgatgggcat cccggaaccg
     2521 gtccgcgaca tagccgagca tgtgcttgtg gtgatgtgcc tgatgccagc tctgatcgtg
     2581 ttccggaact actatcacgg ccggctgatg gtcgagcgcc ggacgtcggg catggccgct
     2641 ggcggcattc tcagagtcat cgcgatactt cttgcggcgc aggcatgcac gatgatgggc
     2701 tggctgaact acctgagcgc gtcgttcatc ctgattctgg gctttctggt ggaagcgctg
     2761 gtggcgctcg tagcggctca gaaaggccag ggactgcggg caaatgccac cgcgaatatg
     2821 tagcctacgc ttgccagagc accagcgaac ccagcgagct tcagcgccgt cgagctcgcc
     2881 cgcatcgtga ggactccgaa tccgatgtag gccaggagcc cggcgagctt tgctgccagc
     2941 cagccttcca gtggcgaaac gctgagctgc acggccagcg ccacaccgag cacgagcagc
     3001 gccgtatcga tgacgtgcgg cgagatacgc acccacttct ggttcttcag cggagagtcg
     3061 accagggacc agattcccct gaccacaaac ccgagcaccg tcaggtaggc aagaatcaga
     3121 tggatcgatt tcagcattgt ctctccccgg gcggtcctcg atcacttcgt ttatgatcgg
     3181 cctcccagcg cattcaagca acccgacacc tcggaaaact atctatgaat attgatcagg
     3241 ccatcgacca ggccatgcgg ccccttgcgg acgcggtgtc cggattcatc ttcttctctg
     3301 tgcccgttct cggaacccag atcccgctga tcgtggtctg gcttatcctc ggggggcttt
     3361 tcttcacagt gtatctgcgc ttcgtcaaca ttcgcggctt cgcccacgcc atcgggctgg
     3421 tcagcgggcg ctaccacaac gaccaacacc cgggtgagat ctcgcagttc caggctttga
     3481 cgactgcggt atcgggcacg gtgggaatag gcaacatcgc cggggtagcc gtggccattt
     3541 ccatgggcgg gcccggcgcg acgctgtggc tgatcatcgc cggcctgctg ggcatgtcga
     3601 cgaagttcgc cgaatgcacc ctcgggctca agtttcggcg gcatcacgag gacggctcgg
     3661 tttccggagg cccgatgtat tatctcgagc gcggcctggc ggagcgcaac tggccgcggg
     3721 tcgggcgcgg gctcgggctg ttctacgcgg catccatggt catcggctgt ctcggtatcg
     3781 gcaacatgtt tcagtcgaac caggcggcag ccatcttcat agacgtcagc ggcggcgatg
     3841 cgagctactt ccaggacaag gcttggctac tgggcctgat catggccggc gcggtagggc
     3901 tggtaatcat cggcggcatc cgttccattg cgcgcaccac ctcgaggctg gtccccggta
     3961 tggcgctgct gtacgtgatc tcggcgcttg ccatcatcgc catcaacgcg gccgaactgc
     4021 ctgccgcctt cgccgccatc tgggcgggcg ccttctctcc ggagggtata gccggaggcc
     4081 tcatcggcgt catgatcatc ggtttccgca gagccgtgtt ctccaacgag gccgggctcg
     4141 gctccgctgc catcgcccac tccacggcga agaccgaacg cccggtgagc gaaggctacg
     4201 tcgctctcct ggagcccttc atcgacaccg tcgtgatctg caccatgacc gccctggtca
     4261 tcatcacgac ggtgtacgaa cccgggctga ccagcggcaa cgtgagcggg atcgagctca
     4321 ccacccgcgc gtttgcgagc acgctgtcat ggtcgccgat accactgtcc atcgccgcca
     4381 tcctcttcgc gttctccacc atgattgcct ggtcctacta cggcctgaag gccttcacct
     4441 atctggtggg ccaccaccgg gtcgccgacc tgggcttcaa gatcttcttc ctcgtcttcg
     4501 tggttctggg cagcagcgtt cagctgggtg ccctggtgga cctctccgac gcgctcgtgt
     4561 tcgtggtcgc gatccccaac ctgctggggc tgtacctgct tgcccccaca gtgcgggccg
     4621 agctgagggc ctatgaatcc aacatgccgg tgcccaacgg cgcggcgccg gctcccgaca
     4681 gcacctgagc cgggcgcgtt ataatgcgcg agctctcagc acgccggaac gctttatcga
     4741 caatggaagg acccacctcc cacgtttact tctcccagcg gctcaggctg cattacgtgg
     4801 attggggcaa ccccgacgct ccacccatgc tgatgattca cggcggccgc gatcactgca
     4861 ggaactggga ttgggtggcc cggtcacttc gcgaccgcta ccacatcatc gcccccgacc
     4921 tgcgcggtca cggcgactct cagtggatgc tcggcgggag ctacaaccag atcgactacg
     4981 tgtacgacat cgcccagctg ctgactcaga aggacatgac gccggtgacg atcatcgggc
     5041 actcgctggg tggctccatc gcgctcactt actcgggtct gttcccggag accgtgaaga
     5101 agctggtagc catcgagggc atggggccgc cgcccgagtt catcaaggaa cggctcgccg
     5161 taccagtggc ggaccggctg caccagtgga tctccgatct gcgcgagctt tcgggaagga
     5221 tcccccgtcg ctacgcgacc ctggaggaag cctacgagcg catgcagacg gaaaatcctc
     5281 acttgagcga ggagcaggca cggcacctga cgatacatgg cagcaaccag aacgaggacg
     5341 gcacctacag ctggaagttc gacaactacg tacgggcttt cccgcccatc ggcatcgact
     5401 tcgaggagca gtacaagctc catggccgca tcacctgccc caccctgctg attcgcggca
     5461 ccgagtcctg ggcgtccgac cccagcgagg acggccgcgc cgcgcgcttc cagaacgcca
     5521 ccgtgcgcaa catcgaaagg gccggacact gggtccacca cgatcagctc gacgagtttc
     5581 tggaaatcgt ggacggtttt ctcgccgact gagaatgagt cagcgcgcgg tcattctgca
     5641 ccgctccccc tggcagggcg tgttccacgt aaccggcggc ggatcgggtc tgctggccga
     5701 gctgctgacc acgcccggcg cctcgggtac cgtcctggag gcggtggttc cgtacgccca
     5761 ggcagcactg gccgacctcc tcggccgcgc gcccgaccag gcctgctcgc cgtccaccgc
     5821 ccgctccctt gccatgagcg ccttcgaccg cgcccgggat ctcggggccc gcgagccctt
     5881 cgggctcggt tgcacggcga gcctggcaac cgaacggatg aagcgcggga aacaccgcgc
     5941 ctgcatagcc gtgcagacga cgaccagcac ctacagcgcc acgctgcagc tggaaggcga
     6001 ccgggccagc gaggagcgcg atctggtgga gctcatctgg cacgcgctca gccggacgct
     6061 agagctgcac ctggagtcgg caccgcccga tactttcgac ggcggtctga ccagcgcgct
     6121 ggaacactgg cgcgcgctgg tgctcggtga ggagaccgcc cacgccaccg caccgcacga
     6181 cggccgtctg ctgctgcccg gctcgttcaa tccgctccat cgggcgcacc gccggatgct
     6241 cgagctggcc gagcatctca ccggcctcga gggggcatac gagctgtcga tcgtgaacgt
     6301 ggacaaaccg ctgctggact acacggaaat cgaggagcgg ctcagccagt tcgaggcgcc
     6361 cgtctggctg acccgactgc cgactttcgt cgacaaggcc cgttacttcc cgggcgctca
     6421 ttttgccgtc ggcgtggaca cgatcatccg catcaccgaa gccggctact acggcagcca
     6481 ggccgaccgg gacgcggccc ttgacgagct tgccactctc ggtacgcgct ttctcgtctt
     6541 cggcagaatt ctgaacgacg ccttcctgac gctctccgat ctggacctgc cggcgacgtt
     6601 ccgggcgctg tgccgcgagg tgaccgaggc ggagttcaac gagccgatct cctcaacgga
     6661 gatccggcga cgctagccca acggggccgc tcagccgccc tcgagctctt ccacccgctt
     6721 cggccagttc tccttgagca ggcgggacag ggaccggtcc gccagcaggc gcccgcccgt
     6781 ctggcatcga gcgcagtagt tggcctcgtt ctcggcatac acgatgcgct gcacgggagc
     6841 gccgcaaacg ggacacggct ggcgatactt tccgtgcacg gccatctccg gatggaaagc
     6901 ggtcaccttc gacgggaagt cgtccccggc cgccgcccgg agacggtccg tccactcgcc
     6961 aagcacgccc ttggttgcct catggagccg ctggatctca ccgtccgcca tgtcccgcgg
     7021 gcgcttgaac ggcgacagcc ttgcccgcag caggatctcg tcggagtagg cgttgccgat
     7081 gcccgcaaag acgtgctgat ccgtcagagc ccgcttcagc gtgtggttgg attcgcgcag
     7141 tcgagacgca aaggcggcaa gatcggtcga gaggacctcg agaccgcccg gattcagcgc
     7201 tccaagggcc tgggcgccgc gcaccagccg cagcgacgcc cgcttcttgc tgctggcttc
     7261 cgtgagcagc aggctgcccg cgggaacgtc gaaggcggcc agcgcgttgg gttttggcag
     7321 gggcgcgctc ggcttctgcc acttcagacg gcccgcgatc atgaggtgaa cgaccacgaa
     7381 gtgctcgccc tccagctcca tgaccagctg cttggcgagc cgatggcaac ccaagatacg
     7441 ccggccgcag acctcgtcca ccgtcggttc gaccgagcgg agcacgaaag ggctggcaat
     7501 gcggatggcc tcgagcggct ggcccaccag tcgcctctcg agatgctcga cgtagacggt
     7561 caggtcgggc agctcaggca ccgagggcct ccggttcccg gtagcggatg gccgttttcc
     7621 agcggccgcc gcggaaccgc gagatgagca ggacgccctt gagcaggtag tcgccgatca
     7681 gtgctgcgta cacccagaac acgggcacgc cgagataggt ggccagggcc gcgagcccgc
     7741 agcgcatgcc gatgagcccg agaaaggtcg ccatgagcgg gaagcgggtg tcgccagcac
     7801 ccctgagcgc gccgccgatg gtgaactcga cggccagcag cggcatcatg gcgccgagga
     7861 tgtaggtgaa ctgcaccgtg tagtccacgg taaggggctc gtcacctatg aaaaagacgg
     7921 ccagctcctc tgcatagaag cagacgaccc cccccaagcc acccatggcg agcaccgcaa
     7981 ggcccgtgga gcgccagccg ctgcgggcgg cgccgtccgg atcgttcgcg ccgaggtgct
     8041 ggcccaccag cgtcgcgccg gcgatggaga agccgaaccc gacggtcatg cacaccatga
     8101 gcatgttggc gccgatgttg taggcggcga aagcttcggt accgtagaag tttccgatca
     8161 gcatcaggaa gatgaagaag ccgagctgga acaccccctg ctcgaccgcc gccgggtagc
     8221 cgatgtcgag aagctggcgc aaccgcgcct tgcgccacca gccgctcgcc acgtgcttga
     8281 cccggaactt ctgtttgacc cagagcccga gaaggacggc cccgccgatg gagaacgcga
     8341 tgccggccgc aatggctgct ccggcaaccc ccatgcgggg aaaaccgaac tcgccgaaga
     8401 tgaacacgta gagcagcgga acgttgacga cgttgaccgc gccgctgatc caaagaggcg
     8461 accaggcatc tccagatgcg cgcagcgcgg cgcccaggat gaagttgacg gcgaaggcga
     8521 cgttgaaaac acacagccag cggatgttct ctgaggccat gccaagcgtt tccggatcga
     8581 gcccgaagac gccggcaatg acaggggcga aaaacagacc gatcaccgcg accacgaccg
     8641 agaaggcgcc ggcgagaacc agcgaggcca tggtgacgcg gctcgcctcg acgtagtccc
     8701 cggcgcccca ggctctggcc actagagccg tggtgcccgc gctgaccgcc atcagaatgg
     8761 cctgcatggc aaagaagaca cgctgccctg cgcccacggc cgcgagcgcc tgggcaccca
     8821 gctcgccgac gaacttggtc tgcacaagac caacgagggt gaatgacagg ttgctcagga
     8881 tggacggaaa cgccagttcc cagatgcccg gtcgccgtcc ctgaagttct cctgcctgac
     8941 cgccttcgga catctgcctc cttctcgaac gcaccaggtt gcttatggaa tgcccgccgc
     9001 gctggctgct aatatcctcc gctcgctcga attcagcaaa cggggataag gatcttgact
     9061 gaatacaccg acatcggcgt gacccaggac ggccacgtca ctaccgtgga gatacagcgt
     9121 ccgccaaaca atttcttcga tcacagcctg atcaggcaga tcgccgacgc gttcaacgca
     9181 gcagacgagg atccgaactg cagggccatc gttctggcag cacagggccg gcatttctgc
     9241 gcgggggcca atttcggcag cggaaaggac gacgactccg gaagcgagga ttttacagag
     9301 gagggctttc gcaacaccac gggcattctc taccgcgagg ccgcgcggct gttcggcagc
     9361 aagaaaccgg taatcggcgc gatccaaggc gccgccatcg gcggaggcct gggtctgtcc
     9421 ctggtcccgg attttcgcgt cgcctgtccg gaggcgcgat tcggagccaa cttcgtcaag
     9481 ctcggcattc accagggctt cggcatctcc gtcagcctgc cccgcatcat cggtcagcag
     9541 gctgcaagca tgctgctgct gacaggccgg cgcatcggcg gcgaagaggc gttcaagctc
     9601 ggcctggtgg acgtactgac cgacgccgag agcctgcgcg acgaggcgat gaagctggcg
     9661 caggaaatcg ccgagaacgc cccgctggcc gtcctgtcag tcaggggcac catgcgcgcc
     9721 gggctcgctg aggccgttat cgaggccacc gagcacgagc tcaaggagca gcaatggcta
     9781 cgcgccaccg aggacgccca ggaaggcatc cgctccgtgg ccgagcgtcg gcccggcaac
     9841 ttccagggcc gttgaggtga agctcggcaa gctcggcgtc tggtactttc tcgacggcct
     9901 gccggcggat gccgcggccg ccgccgctca gcgcatcgag gaactgggtt acagcgctct
     9961 gtggataccg gaggcgacgg ggcgcaaccc catggttcac gcctcgtggc tgctggccaa
    10021 caccaagcag ctgatcgtgg ccacgggaat cgccaacatc taccaccggg aacccggcgt
    10081 cacggtctcg gcgcaaaagg ccctggccga gcagtcgggc gaccggttcc tgctcggaat
    10141 tggtgtttct cacaagccct tcgtggaggg cgtgcgcggc ctgacttacg gcccgcccgt
    10201 ggcgagcatg cgggactatc tggacaagat ggccgctgcg ccctacacga gcgttccgcc
    10261 ggccgaagac ccgcccacgg tgatcgccgc gctgggcccg aagatgctgg ccctggccgc
    10321 cgagaagact gcaggcgccc acccctactt cgtttcgccc gaccacaccg ccatggccag
    10381 cgaagtcctc ggtccggaca agtggctgtg cgtagagcag aaagtcattc tggagacgga
    10441 tccttcccgg gcccgggagc tcgcccgccc ggcggcgcag atgtacctgg ccctgcccaa
    10501 ctaccgaaac aactggttgc gcatgggctt caccgaagcc gacctcggca acggcggttc
    10561 cgatcggttc atcgacgcca cctttgcctg gggcagcgcg gcccaggttg aagcccgcat
    10621 tcgcgagcat tttgatgccg gcgcgaccca cgtctgcatc cagcccgtca atcccaacgg
    10681 tcagtttggc gatctacact gggaagccct ggaggcgctc gcgccatgag cagcgactac
    10741 acaggcaacg tagtcgtcgt cacgggcgcg gccggcgccc tgggccgtgc cgtggtcgag
    10801 cacttcgccg caagcggcgc caccgtggcg cagctggacg tggtggccat cgacaacgcc
    10861 cacttctccg acacctgtga tctgaccgac ccggacagct gcaggcaggc ggtcacccgg
    10921 atcatcgaga cgctggggct catcgacgtg ctggccaaca tcgccggcgg cttcaccatg
    10981 ggagaggccg ttcacgaaac ctccgaggag acctgggatt tcatgatggg cctgaacgcg
    11041 cgctcggtgc tgaacatggc ccgggccgtg gtccccatca tgcttcagca gggggccggc
    11101 aagatcgtca acgtcggcgc cggcgcaggg ctcgccggag tcgcaaacat gggtgcctac
    11161 agcgcgtcta agagcgtggt catccgattg acggaagcca tgtccgcgga gctcaaggag
    11221 cgacgcatca acgtcaactg cgtgctgccg tccatcatcg ataccgagcg caaccgggcc
    11281 gacatgcccg acgccgattt ccaccgttgg gtgacgcccg cggccatggc cggggtcatc
    11341 ggctttctgg cgtcacctgc agcggacccc attcacggcg ccgccgtgcc cgtcagcggg
    11401 ctgagctgag gggcgcgggc cgaccggccc gcgacgctgc ggatcaggct ccaaatacct
    11461 ggttcccgtc catccacgtc tggctcaccc cgatctcgga gatggccttg ggctcgacct
    11521 tcctcgggtc gtcctccagc acgaccagat cggccagctt gccttccgct aggctgccga
    11581 cctcggtctc cgagtgacac tgccatgccg cgttgatggt gacggccctt atcgccgcct
    11641 ccacggaaac gcgctcgtgg ggcgcaagtg tggtgtcggg ctccttccag aggtcgcggg
    11701 ccaccgcaac ccggatcttg tgcagggtgc caagcggtga caccggcgcg tcgctgtgca
    11761 tcgtccaagt gatgccggca tcttcgacgc tccggcagcg atcgagcagc tgcactttct
    11821 ccgggccgaa caccttgtcg cgcatggcgt gaccccaata gtgcacgtgg ttgatgagaa
    11881 agctggggcc catgcccagc tcgcgaatgc gctggatctg cccatcgtgg aggatcgacg
    11941 cgtgctcgat gcggcagcgc agctttccca cgtccacgcc cgcatccgcc gccgcctgca
    12001 ccgcgtccag aatgctgtcg atcgcggcgt cgccgttgcc gtgcagcacc agcggccagc
    12061 ctcgacggcc acggctgagc accatttcat tcagcgcttc gggcgcgacg tagtacatgc
    12121 cccgattgtc cgagtctaga tacggctcac gctgccggcc cgtgaaaccc tggttggacc
    12181 catcggtcac gatcttccag cccacaatgc gcatcagggc gtccccgtca ccgaaggcaa
    12241 cgccgttgtc gtcccactcc gcggccgtct cggaatagag cgaggcgcga atccgcgcgc
    12301 tcatccggct cgaggcaaac atctgccggt agaagtcgat atcgcccatg ccggcaatgc
    12361 ccccggtggc ctggtcacag agggtggtga tcccgagcgc ggccgcctct tcgccgaccc
    12421 gcaacccggc ctcaacgaac ccggagccaa gaaccgcgat gagcgcctcg ttccgcatca
    12481 tcaccggctg gtaggcgacc tgggacatgg cgccgttggg ccgcccatcg gggtcacgga
    12541 aatactcccc gccgggcgga tccggcgtgt ccctgtcgat gccagcgaac tccagcaggc
    12601 gggagttacc gtaggcgaga tggcccgagg cgttcagcac aaagacgggc cggtctgttg
    12661 acacggtatc tagctcccgg gtcgtcagca tctcgggacc cgtctgaagg ctgggatcga
    12721 actgacgggc aacgatccac tcatcagccg gcgtctccct ggcaatgagg cgcagagcgg
    12781 cgagcgcgtc ctcggtgctt tcgtgtttga acgggccgat gtcaggccag tggcccagcc
    12841 cggccatcag cgcgaagtgc atgtggggtt cgatgaatcc cggcaggagg acccggccat
    12901 cgagatcgac cacctggggg tctttccccg ccgccgccag cacgtcgtcg tcatccccga
    12961 cggcaaggat gcggtttccg cggatggcaa tcgccctctg ctcggagaat cccgcatcca
    13021 ccggcaacac cacgccgctg cggaaaacga cggcgcggcc gggacccgca acctgcccgt
    13081 cgccggcccc ggcagacctc ggacgggtat cagcaccgca accccacagc gacaagccgg
    13141 cagccgtcag ggcgccggcc tctacgaatc gtcttctgtt aaccatcccg gcgggcctcc
    13201 aaaatctgtt cttccctcga cttccggtga gtcctgccgc caacgggtca cggtctcctt
    13261 tgctgcgtca gcgtacacgg cgagccagcc gaaacgaagc aacccgatac atgcacggct
    13321 catgctggcg gcaatggaat cctctcggta tccttcgcgg cgcgtcgaga tctcgagagt
    13381 cgagccagcc tcgattcggg cagcaccacg ctgccggatt gcgagcccga cgctcaaccg
    13441 ttctgacaca gaggtagacg atgacatcgt atgatccgtt cctgcgtggc ccgcatccgg
    13501 tcggcgtgcg cacctttcac tggaccgaca aaacaaggca acgaacgttg cccgtggaag
    13561 tgtggtaccc ggccgacgac gagcacgccg ggcaggatct cgacccggat acccaggatc
    13621 acttccagcc catgagcctg tcgccaccgg taccccaggc agccgttcgc gacgcggcac
    13681 cggccgaggg tgagccccgg cccctgctga tcttctctca tggcttcggc gggaccggcc
    13741 gccagacgac acacctgtgc acccattggg ccagtcacgg ctacgtggtc gccgccatgg
    13801 accacgtcgg caacaccacg gaggacgtga tggcggcggc cgcccagcca gtggatccag
    13861 ggaccctgct cgagcgcctg aaatcgtttg ccgcagaccg gccagtggac gtcagcttcg
    13921 tgatcgaccg gatggtggcc ggcgatgccg gcctgaccgt cgatcccgac cgcgttggcg
    13981 tctccgggca cagcttcggc ggctggacca ccctggcatc ggccggaatg gatccacgca
    14041 tccgcgccgc cttgcccctg gcaccggccg ggggccaatc gagccagtgg cagtcgagca
    14101 tcgccgaccg cctggcagac gcgctcgcca tgagctggga gcgcgaggta cccacgctgt
    14161 ttctggtcgc cgagcacgac gtggtcctgc cgctggacag catgcacgac ctcatcgagc
    14221 gaacgccgca cccgcgccgc gccgtgaatc tactgaacgc cgatcacttc cacttctgcg
    14281 atcgggtgga gcagacccac gacatggtga ggatgatggg acagatgatc ttcggcacgc
    14341 gcagcgaagc ggaccaggaa gcgccctccg cgctggcggc catgaagccg agcagcgagc
    14401 tgtgccctgg cgatcacgcc tacgcgctgc ttcaggggct gggcctggcc catatggacg
    14461 cccacgtgag ggagctcgac cgggcccacg cgctgctggc gggcgacctg gcgtcgctga
    14521 tggccgaacg gggcgtggcg gtcaaggtca tggcctgagc agcgcacgcc ggcacccccc
    14581 gttcccccgt ccgggtgacg gcccggcaga tcagaagacg aggcgaccca ggctttcggc
    14641 caccagcgcc ggcttttcct gaccctcgat ctcgaccacg atctcgttca tggtctcgat
    14701 catgccgccc ttgatctcgg cgcggcgcgg cgtgctggtg gcgcggatcc tgtcgcccac
    14761 ccgcacgggc gcgggaaaac ggatacggtc gaagccgtag ttgatgctga gcttgtggcc
    14821 ctccagcggt ggctgggagg ccgtctcaga tcgcggcagg tggcccatca cggcgatggt
    14881 caggttgccg tgggcgatgg gcgcaccgaa cggcccctgc ttggcccgct cgacgtcgac
    14941 gtggatccac tggtgatcca tgctgacgtc ggcatagtcg ttgacccgat cctgagtgat
    15001 ctcgaaccag tctgaggagg ggttaggctg gtcgagacgc tgtcgcagaa tctccagggc
    15061 gttgttcatg ttggtttcgg tgtcggacat gatctcattc tccggtaatc gtcaaaaatc
    15121 aaacgggcgg ggcccaccgg ctggcatcgt cgttcgcatc gagggagccg atgaggtcca
    15181 cgatcgggcc ggcgccgctc agcgaagcag gttcgaagtt cgagcgagcg cctgctcggc
    15241 cccgtaaacc agctcgtcga atatttcccg ggccgggcgg acggccttta tcatgccgat
    15301 gccctggccg gcgaaacccg ggttgatgtc gccccggcca tcgcggttcg ccgcggccag
    15361 caccggctgg gagaccaagc cctggaaggg catcggcagg ggatcccgct cgccccgcgc
    15421 ccagtaatcg gtccatttgc tgcgaatgat ccgtgccggc ttacccgtca cggaccggga
    15481 cacggaggtt ccctcctcgg tggcatcgac gatggcctgc ttctgaaaat cgaagatgtt
    15541 ggcttcctgg gacgcgagaa acgcggagcc gatccagacc ccctcggctc cgagcatcat
    15601 ggctgcagca atgcctcggc cgtcgacgat gccaccggcg cccagcaccg gcgtatcgcc
    15661 caccgcgtcc accacctggg gtatcagcgc catggtgccg atgggcgaat tgtgaccgcc
    15721 gccgtcgtga ccctgggcca cgatgatgtc gagcccggag gacttcacct gaatcgcgtg
    15781 ccggacggcg cccaccacgg ccatcacctt ggtgccgttg gcgcgcagct cgtccatcca
    15841 cggtccgggg ttgcccagac cggacgcgta aacggggacc cgctcctcga tgaccacgcg
    15901 catctgcgcc tcgaagaaat ctttcgtgaa cacggcgggc tcgtcggacg ttcgcggcgg
    15961 ccgcggcgac tcgctcgcca ccggctccag gccttcgcgt tccatgaact cagccgcgta
    16021 ggcctgggtc tcaggcagca gatccatggg cgtcggcccc tcggtctcgt cgtagttggc
    16081 gcgccgaacc gacgcaggca gcagcgtgtc gacaccgaag ggtctgtcgg tgagctcgcg
    16141 cgtctcgcgg atccagcgcc gcaggagctc cggaccacat cccgcggctc ccagcacacc
    16201 gagcccgccg gcgttggata ccgccgcggc cagggccggg cagctcgccc cgcccattcc
    16261 tgcgagaacg atggggtgct cgatttctag aacttcgcag acgcgcgtct tcagattcat
    16321 cacagtccct cgaattgacc atagccgccg cggaagaaca ggagcggcgg cttgtcgggg
    16381 ttgacgatgc tgacctcacg caccagcccg acggcaagcg tatggtcgcc cgcatcgtgc
    16441 tcggcatgca ggtcgcattc gacgtaggcg atcacgtcgg ccagaacggg ggatcccgtt
    16501 gcccccggcg accagtcgag gctggcaaac ttttcgaccc ccgattgcgc gaaaacgtcg
    16561 cagacggcct tctgatcctc gccgagaatg ttgatgcaga agctgcccga gtccctgagc
    16621 ttcggccagc tggtgctcgt cttggcgggg cacagcacga ccagcggcgg gtccagcgaa
    16681 accgagctga aggactgggc ggcgaaaccg gccggctcgc cctctcggca gcccgtcgca
    16741 atcaccaccc ccgtgcagaa ctgtcccatg gtgtcgcgaa acacccgttg gtcgatttcc
    16801 gacatcccgc tcccctggga tcgaagagcc gcggactata accttccgct gctgccctgg
    16861 ccaacgagcc cgtttccttt agaattccga gtccggcaga tagggcgcag cagaggttca
    16921 gacagcggtg acagatctta acgagtttcg caaagaggtc agaaactggc tagaggagaa
    16981 ctgcccgccc agcatgcgca cgtccacgcc cgagggcgag accgtctggg gcggccgccg
    17041 ggagaagttc gcgaatccgg actccaggat ctggctggac cgcatggccg agcgcgggtg
    17101 gacggcaccc acgtggcccc gggagtacgg cggcggcggc cttacggccg acgaggacaa
    17161 ggtgctccag caggaactgc gcagcatcaa tgccaggccg gcgctgcaga gcttcggcat
    17221 ctggatgctg gggccggccc tgctggagtt cgccagcgag gagcagaaac tcgagcacat
    17281 accgcagatc gttcgcggcg agatccgctg gtgccagggc tactcggagc cgggctacgg
    17341 ctcggacctg gccgggctgc agaccaaggc ggaggaccag ggcgaccact tcctggtcaa
    17401 cggttccaag atctggacct cctacgcgga cgttgcggac tggatcttct gcctggtccg
    17461 caccgacaac gacgcgccga agcacgaggg catcagcttt ctcctcttcg acatggactc
    17521 cgagggcgtg accacctccc ccatcgagct gatcagcggc gcctcgccct tctgccagac
    17581 gttcttcgac aacgtgaggg tgcccaaggc aaacctggtg ggcgggctca acaagggctg
    17641 gagcatcgcc aagcggctgc tgcagcacga gcgccagatg gtggccggga tcggcgggat
    17701 gagcgccctc ggaggcaccg gccgccggct cgaggacgtg gccaaggcgt acgtcggcga
    17761 ggcacagggg cgcatcgggg acccggccgt ccgcagcagc gtcatcgagc accgcatgaa
    17821 cgaccgagcc tttcagctga ctctggagcg cagcggggac gaagccaagg cggggcaggc
    17881 ggacggcaat ctggccagca tgttcaagta ctatggcacc gagcagaaca aacgaaagta
    17941 cgagctgatg ctgcaggcca tgggcaccca gagcatgggt tgggagggcg aggctttctc
    18001 agccaaagag ctcggcacca cgcggcaatg gctgcgctcc aaggcgaact ccatcgaggg
    18061 gggaaccagc gaggtgcagc tcaacgtgat cgcaaagcga gtactggggc tgccggactg
    18121 aacgcgggag ttcccggacc gacatccggc caaggaggag atcgtggaaa ccatcatcgt
    18181 gctcgtcatc atagcggcgc tcgctttctg ggtgatcgcc atctacaacc ggctggtgac
    18241 cctgaagaac cgcttcaaga acgccttctc gcagatcgag gtgcagctca agcggcgcta
    18301 cgacctcatc ccgaacctgg tcgagaccgc caaggcgtac atgtcacacg agcgggagac
    18361 cctcgaagcg gtgatcgccg cccgcaacca ggccctggcc ggcctgcaga cggccgctca
    18421 ggaccctggc aacgccgatg cgattcgtca gctggcaagc gccgaagggg cgctgggcgg
    18481 cgcgctgggg cggctgaacg tggtgatgga ggcctacccc gacctcaagg cgaaccagaa
    18541 catgatgcag gtttccgagg agctgacgag caccgagaat cgcgtggcgt tcgcccggca
    18601 ggcgtacaac gaccaggtga ccgactacaa cacctacaag cagagctttc ctgcgatgct
    18661 gctcgccggg cccttcggtc acgctggcga cgcggccctg ctggagtacg aggacagcga
    18721 ggcaatgcag gaggccccga aggtttcctt cagctgatcg tccccggggt cggatgaact
    18781 tctttcgcgc ccaggacgac gcgaagcgca agacctggcg cctggcgctg ctgttcgcgg
    18841 ccgccgtggg ctcgctgatc gtgctcacga acctcctgct gatgggcgtc taccgctgga
    18901 ccacggacgt cggagtggcc acctcgccgg acgctatcgc ggccttcacc aacgtgccca
    18961 ccgagcagtg gctcatcgtc accgcagccg tggtcgccgt ggtggggata gcgagcctgt
    19021 acaagtacct ggtcattcgc gacggaggga agaccattgc agaggcgctc ggaggccgcc
    19081 ggatcaacgc caacaccacc gatccggccg aacggcgcct gctcaacgtg gtcgaggaga
    19141 tggccatcgc ctccggcacc gccgtgcccc ccgtctatct cattccggag cacagcatca
    19201 acgccttcgc ggccggattc acgcccgatg acgccgtgat cgggatcaac cagggcaccc
    19261 ttgaccagct gaacagggac gagcttcagg gggtggtcgg ccacgagttc agccacatct
    19321 tgaacggcga cagccgcatc aacctgcgcc tgatcgcgat gctgcacggc atcctcttca
    19381 tcggcatcat cggttacggc atcctccggg gcatggccca cggcggcaac cgccgatccg
    19441 ggggcaacgc ggcggtgccg gtgatggccc tcggcctggg gctgctgatc atcggctact
    19501 cgggcacctt cttcggcaat ctcatcaagg ccgcggtgag ccgacagcgc gagtatctgg
    19561 cagatgctgc ggcggtgcag ttcacgcgaa accccagcgg cctggccaat gcgctcaagc
    19621 gcatcggcgg cctgcccgtg gggtcgacca tgtcgggatc gaatgcccgt gagatcagcc
    19681 acatgttctt cggccagtgc tctcgtccgt ttttcagcgg tctgatgtct acccatcccc
    19741 ccctcgaaga ccggatccgc gccatcgaac cgcgctggga cggtcggctc ctggaacgtg
    19801 gccaaccgtc cccgacgcgc cctgccggcg ccgcgggatt tgcgggtacc ggcgcagcgg
    19861 gcattcaagt accggggggc atctcggtgg gggtccccgc ggagagcatc gcggacgagg
    19921 tcggccgcct ggaccagacg agcctggaca acgcggtggg catcatcgcg agcatcgatc
    19981 cgaagctgcg cg
//
LOCUS       DBNHKV010000045         7781 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_857370, whole genome shotgun sequence.
ACCESSION   DBNHKV010000045 DBNHKV010000000
VERSION     DBNHKV010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7781)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7781)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7781
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_857370"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="AC16PP_01330"
     CDS             complement(<1..419)
                     /locus_tag="AC16PP_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase family
                     protein"
                     /protein_id="HIR6480292.1"
                     /translation="MLEDVTRFGSKADARQWAWDALDERRLARFPFPPHGRIPNFAGA
                     AAAAMRIFAQAPWSEAGAIKVNPDSPQLPVRFLALQRGIRVYVPTPKLAAGFILLDPA
                     RIPPERFREAAALKTMLRWARPVALEDLPQLDAIVTG"
     gene            479..1723
                     /locus_tag="AC16PP_01335"
     CDS             479..1723
                     /locus_tag="AC16PP_01335"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6480293.1"
                     /translation="MTNFLLQRYPAFGFPRYRKYWLASLASVGATQLITLAQGWLVFE
                     LSGSALQLGILGAAVAAPNIAMTLIGGVFADRFDKRLILVWTSGAIATLLALLAWLDY
                     SGTVRVWHVLAVAAAVSLITGLDWPARVSIFPQLIDRPAFMSAVALNSIIWQATRMAM
                     PALGGIIIAASDTWVLFALGSGGFLTMMIVISGLHVTTSDARHGSPLEQIAEGVRFIV
                     NEELFKWLMLLTFIGMFFAGSYVQIMPVFADVLESGEAGYGYLLSAGGVGAVAGTLLV
                     GGVQEHARLGFVMLGGATLSAALTAVFALVAVGGWFIAALLATFFASVFTSVFMISSM
                     AVLQLVVPDDLRGRVMGIHAIGYSLIPLGGLFMGALAEAAGPALAVLIGCCVFGFAIA
                     GVAMTSTTIRGLDGRMIGPKQA"
     gene            1720..1938
                     /locus_tag="AC16PP_01340"
     CDS             1720..1938
                     /locus_tag="AC16PP_01340"
                     /inference="COORDINATES: protein motif:HMM:NF022596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HIR6480294.1"
                     /translation="MSEVGKWLVLAGALTVIVGLLLQTGWLGWFGRLPGDIRVERPGL
                     SFYFPITSMLIVSAILSVAAWILRRFLG"
     gene            complement(1942..2574)
                     /locus_tag="AC16PP_01345"
     CDS             complement(1942..2574)
                     /locus_tag="AC16PP_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480295.1"
                     /translation="MIYLVVNIVGYLLVALVAGVGVGWLLHGSLAGKASGERRGLGSV
                     GVDDLELAARADAQDEQLRKLAATIRQKEREVEALSLELERRDRRFNAADKLAKISRR
                     LQQEAEQLKEQVRVLGEQLEAAREEAQGSHGASAAMEEVLRSEILELEGRLRESSQEQ
                     DRLAKTLEQENRKVEELERQRDLQNSSLQVLHKQLELARKSREGAAEAGG"
     gene            complement(2571..3239)
                     /locus_tag="AC16PP_01350"
     CDS             complement(2571..3239)
                     /locus_tag="AC16PP_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR6480296.1"
                     /translation="MDLRFSQFEILTAAGAFFLCLAFICVFNEASDIQDDVGIRAQGE
                     AREHGLYWAGVEALGQRVRLTGAAPSDAQRLHAAAAVAALPGVTSVQNEIEVLGPDGA
                     CQRQMDSALAELGVTFKTGRAEIGDSSYPLIAELARTMGACATPIEIAVHTDSLGDAA
                     VNLKLSQRRADAVRKALVQRGVRPDRLVAAGYGETQPVASNARESGRRENRRVEFRVV
                     GGTA"
     gene            complement(3406..6057)
                     /gene="aceE"
                     /locus_tag="AC16PP_01355"
     CDS             complement(3406..6057)
                     /gene="aceE"
                     /locus_tag="AC16PP_01355"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014603513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIR6480297.1"
                     /translation="MAERNPDDWLSEQQEWCDAIDDVLAFRGEDEVKELLRRLQAHVS
                     HRGMILTDAALNTPYKNTIDVRDQPAYPGNIELEMKIGDIICWNAVAMVLQAYDSGSG
                     VGGHIATYLSAATMMEVGFNHVFRGASESYGGDQVHFQAHTSPGVYARAFLEGRLSEQ
                     ALTNFRRELAPGGGLPSYPHPRRLPWFWQMPTASMGLSTPSAIYQARFAKYLESRGLK
                     ANNGGKIWTFIGDGESDEPEVLGTINIASRERLDNLVLVINCNLQRLDGPVRGNGKII
                     QELERAFRGADWHVIKVIWGGGWDAILARDEHGILQERMEQAVDGDYQMYTVSSGDVV
                     REHWVETSPELEELMKTLSDEEIRSIKRGGQDHQKIYAAFARAANVRGKPCVVLLKTV
                     KGDGLGPGTEGSNTVHQKKQLNEQERQELAKRLGIPLTREEISRAAFYRPAEDSAELV
                     YLRDRRHSLGGYLPAREVRCAALEPPSVELFAAMLKGSDRPVTTTAAVVRMLSRLLRD
                     PALGRYIVPIVPDEARTFGMDGLFPQAGIYSPAGQRYQPVDAGTIAPYREAEDGQILQ
                     EGICETGAMASFLAAGTAYANYGLPMIPFYIFYSIFGFQRVGDMIWAAGDMMCRGFLL
                     GGTSGRTTLNGEGVQHQDGHSHLVANTIPNLKSYDPAFAFEIALLVREGIRRMYQLQE
                     DVFYYLTLTNQPYPMPAMPEGVEDGVLKGMYCFSRAPGAVVNLFGSGALMTEVLRAES
                     LLAEHGIACNVWSVTSYNELARQALASERARLLGVGDGDERAYAEALLEGEEGIFVAA
                     SDYQKSLPMSISRWVPGPYTVLGTDGFGLSEDRRVLRGYFEVSAEWIAFAALSSLAQN
                     NRLERDVALGFAAKAGLALDKANPAEG"
     gene            complement(6195..6989)
                     /locus_tag="AC16PP_01360"
     CDS             complement(6195..6989)
                     /locus_tag="AC16PP_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017849031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR6480298.1"
                     /translation="MHLESDVAPEEAAHSLRLLTFNIQVGIRTTKYRHYVTKGWKHVL
                     PHEARAINIRHIADLVRDYDLVALQEIDGGSLRSGFINQVEYIADRARFPYWYTQCNR
                     DLGPFAQHGNGVLSRIAPVHMEDHKLPGAIPGRGAILMRFKLGESTVSLVVLHLSLGG
                     RSRQVQLEYVRELIDAEEHVVVMGDMNSHLAQLLFNSPLSETDLVPADQVHPTYPAWQ
                     PAFALDHVLVSPGLSIDDYEVLDCRLSDHRPIAVTLRLGEPVTIQQ"
     gene            complement(7131..>7781)
                     /locus_tag="AC16PP_01365"
     CDS             complement(7131..>7781)
                     /locus_tag="AC16PP_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HIR6480299.1"
                     /translation="APGPAFAQEWQEGVHYQRLPVSVATADAARVEVVEVFSYACIHC
                     KTFDPALEAWRENQPEDVGFRRVPAIFSAEWETLAKAFYTAQILDLGGAVHDSIFRAV
                     HEEGRDLREPGAIAALFQQVAGVNPQQFAQVFNSFGVRSRIEQAKAHARAYRVSGVPA
                     LVVDGTYRVDGRMAGSNAAMLEVVNHLVAKQRAARGIAAPEPASVEEEDAATSGAS"
BASE COUNT         1326 a         2590 c         2462 g         1403 t
ORIGIN      
        1 cacccggtca cgatggcatc cagctggggc aggtcttcga gggctaccgg ccgcgcccag
       61 cgcagcatgg tcttgagcgc cgctgcctcg cggaaccgct cgggggggat ccgcgccgga
      121 tccagcagaa tgaatcccgc tgcgagcttc ggtgtcggca cgtaaacccg gatcccacgc
      181 tgcagcgcca ggaaccgaac gggcagctgg ggcgagtcgg gattgacctt gatagccccc
      241 gcctcggacc agggggcttg cgcaaaaatc cgcatcgcgg ctgccgccgc gccggcgaag
      301 ttcggaatcc gcccatgggg tgggaacgga aatctggcca gacgacgctc gtcgagggca
      361 tcccacgccc attgacgcgc gtccgccttg gagccgaacc tggtgacgtc ttctagcaag
      421 gcgagtggtg ccagccaata cggtcacggc atactacacg gccgctcaga gagatggcat
      481 gaccaacttc cttctgcaac gctacccggc tttcggcttc ccgcgctacc gaaagtactg
      541 gctggcgtct cttgcctccg tgggcgcgac ccagctgatc accctggccc agggttggct
      601 ggtattcgag ctatcagggt cggcgctgca gctgggaatt ctgggcgccg ccgtcgccgc
      661 gccgaacatc gcgatgacgc tgatcggcgg cgtgttcgcc gaccgcttcg acaagcgtct
      721 cattctggtc tggacatcgg gggccatcgc cacgctactc gcgctgctgg cctggctgga
      781 ctacagcggt accgtgcgcg tgtggcacgt cctcgccgtg gcggccgcag tctctctcat
      841 caccgggctc gactggcccg ccagggtctc catctttcca cagctcatcg accggccggc
      901 tttcatgagc gccgtagcgc tcaactccat catctggcag gccacgcgca tggccatgcc
      961 ggcactgggc gggatcatca tagcggccag cgacacctgg gtgctgttcg ccctgggcag
     1021 cggcggcttt ctgaccatga tgatcgtcat cagcggcctg cacgtgacca ccagcgacgc
     1081 gcgccacggc tcgccgctgg agcagatcgc cgagggcgtg cgcttcatcg tcaacgagga
     1141 gctgttcaag tggctgatgc tgctcacgtt catcggcatg tttttcgcgg gcagctacgt
     1201 gcagatcatg cccgtgttcg cagacgtcct ggagtccggt gaggccggct acggttatct
     1261 gctctcggcc ggaggcgtgg gcgcggttgc cggcacgctg ctggtcgggg gggtgcagga
     1321 gcatgcgagg ctcgggttcg tcatgcttgg cggcgcaacc ctgtccgccg ccctaaccgc
     1381 cgtgttcgct ctggtcgcag tgggtggttg gttcatcgcc gcgctgctgg cgacgttttt
     1441 cgcatcggtc ttcacctcag tcttcatgat cagctccatg gccgtcctgc agctcgtcgt
     1501 tcccgacgac ctgcgcggcc gggtcatggg catccacgcg attggctaca gcctgatacc
     1561 gttgggaggc ttgttcatgg gcgctctcgc cgaggccgct gggccggcgc tggcggtgct
     1621 gatcggctgt tgcgtgttcg gcttcgccat cgccggcgta gccatgacca gcacgaccat
     1681 tcgcggtctc gatggtcgca tgatcgggcc caagcaagca tgagcgaggt gggcaaatgg
     1741 ctggtgctcg ccggagcgtt aaccgtgatc gtcgggttgc tgctgcagac ggggtggctc
     1801 ggatggttcg gcaggctgcc gggagacatc cgggtggagc gcccggggct ctccttttac
     1861 tttcccatca cctcgatgct catcgtctcg gccatcctct cggtggccgc ctggatcctc
     1921 cgacgcttcc tcggctgagc ctcaaccacc ggcctcggca gcgccctccc tgctcttccg
     1981 ggccagctcc agctgcttgt gcagaacctg cagagagctg ttctgaagat cacgctgccg
     2041 ctcgagctcc tcgaccttgc ggttttcctg ctccaacgtt ttggcgagcc ggtcctgctc
     2101 ctgagaagac tcccggagcc ggccctcgag ctcgaggatc tcgctgcgca gaacttcctc
     2161 catggcagcc gacgcgccgt gggatccctg ggcctcctcc cgcgcggctt cgagctgctc
     2221 gccgagaacg cggacctgct ccttgagctg ctcggcttcc tgttgaagac gccgggatat
     2281 ctttgccagc ttgtcggcag cgttgaagcg gcgatcccgc ctttcgagct cgaggctcaa
     2341 cgcctcgacc tctcgctcct tctgcctgat cgtggcggcc agcttcctca gctgctcgtc
     2401 ctgggcgtcg gcgcgagccg cgagctcgag atcgtcgacg cccacggaac cgagccctct
     2461 gcgctcgccg gaagccttgc ctgcgaggga tccgtgcagc agccacccca caccgacgcc
     2521 ggcgaccagg gcaaccagca gatagccgac gatgttgacg acgagataga tcacgcggtg
     2581 cctccgacca cccggaattc aacccggcga ttttcccgac gtcccgattc gcgggcattg
     2641 gaagcgacgg gctgggtctc gccgtagccg gcagctacga gacggtccgg gcgaacgcct
     2701 ctttggacca gagccttgcg taccgcgtcc gctcgccgct gactcagctt gaggttgacg
     2761 gcggcatcgc cgagggaatc cgtgtgcacg gcaatctcga tgggcgtcgc gcatgcgccc
     2821 atggtccggg caagctcggc aatcaacggg tagctggagt cgccgatctc ggcgcgaccg
     2881 gtcttgaagg tcacgccaag ctccgccagc gccgagtcca tctgcctctg acacgcgccg
     2941 tcggggccga gcacctcgat ctcgttctgg accgaagtga cacctgggag cgcggcgacc
     3001 gctgctgccg cgtgaagcct ttgggcatcg cttggcgcgg cacccgtcag cctgacccgc
     3061 tggcccaagg cctccacacc cgcccagtaa aggccgtgct cgcgcgcctc gccctgggcg
     3121 cgtatgccga cgtcgtcctg gatatctgat gcctcgttga acacgcaaat gaacgccagg
     3181 caaagaaaga atgcgccggc ggctgtgagg atctcgaact gtgagaaacg aaggtccatt
     3241 ttccttactt acaggattcg agccgagttg gtcccttggg ggattttgaa gcgatggcgg
     3301 ctcgttttac gtgaaccgat tctcaactta cccgacccgc gcgggcaaag gcaacgccaa
     3361 tccggggccc catcggcgca tgccccagag cacatcccgc gcccctcagc cctcagcggg
     3421 gttcgccttg tccagggcaa gccccgcctt ggccgcaaag cccagcgcca cgtcacgctc
     3481 gagccggttg ttctgggcca gggacgacaa cgcggcgaac gcgatccact cggcggaaac
     3541 ttcgaagtag ccgcgcagaa cccggcgatc ctccgacagc ccgaagccgt ccgtgcccag
     3601 cacggtatag gggcccggca cccagcgaga aatgctcatg ggaagggact tctggtagtc
     3661 cgaggcggcc acgaatatcc cttcttcccc ctctagcagc gcttcggcgt aggcccgctc
     3721 atcgccgtcg ccgacaccga gcaggcgggc tcgctccgag gcgagcgcct gccgggccag
     3781 ctcgttgtag ctcgtaacgc tccagacgtt acacgcaatg ccatgctcgg caagaaggct
     3841 ctccgcccgc aacacctcgg tcatcagcgc gccgctgccg aacaggttga ccaccgcccc
     3901 cggagcacgg ctgaagcagt acatgccttt gagaacgccg tcctccacgc cttcgggcat
     3961 ggccggcatg gggtagggct ggttggtcag ggtgagatag tagaagacgt cttcctgcag
     4021 ctgatacatc cgcctgatgc cctcacgaac cagcaaggct atctcgaagg cgaacgccgg
     4081 gtcgtagctc ttcaggttgg gaatcgtgtt ggcaaccaga tgggagtgcc cgtcctgatg
     4141 ctgcaccccc tcgccgttca gggtggtacg ccctgaagtg ccgccgagga gaaaacccct
     4201 gcacatcatg tcgcccgcgg cccagatcat gtcgccgaca cgctgaaaac cgaagatcga
     4261 gtagaagatg tagaacggaa tcatcggcag gccataattc gcgtaggccg tgcccgcggc
     4321 caggaaagac gccatggcgc ccgtttcgca gattccttcc tggagaatct gaccatcctc
     4381 ggcctcccga tacggcgcga tggtgcccgc atccaccggc tggtagcgct gaccggccgg
     4441 cgagtagatg cccgcctggg gaaaaaggcc gtccatcccg aaggtgcgcg cctcgtcggg
     4501 aacgatgggc acgatgtatc ggccgagagc cgggtcacgc agcaggcggg acagcatacg
     4561 gaccaccgcc gccgtggtgg tcaccggcct gtccgatcct ttgagcatgg cggcaaaaag
     4621 ctccaccgac gggggctcca gcgccgcgca gcgcacttcc cgggccggca ggtaaccgcc
     4681 caggctatgg cgccggtcgc gcaggtagac cagctctgca gagtcctcgg ccgggcgata
     4741 gaaagccgct cgcgatatct cctcccgggt gagcggaatg ccaagcctct tcgcgagctc
     4801 ctgtcgctcc tgctcgttga gctgcttctt ctggtgcacc gtgttgctgc cttcggtgcc
     4861 tggcccgaga ccgtcgccct tcaccgtctt gagaagcacc acgcacggct tgccgcgaac
     4921 gttggccgcc cgggcaaacg cggcatagat cttctggtga tcctggccgc cacgcttgat
     4981 ggaccggatc tcctcgtcgg agagcgtctt catcagctcc tcgagctcgg gggatgtttc
     5041 cacccagtgc tcgcggacca cgtcgccgct ggacaccgtg tacatctgat agtcgccatc
     5101 cacggcctgc tccatacgct cctggagaat gccgtgctcg tcccgggcaa gaatggcatc
     5161 ccagccgccg ccccagatca ccttgatcac gtgccagtcg gcgccgcgaa aggcgcgctc
     5221 gagctcctga atgatttttc cgttgcccct gaccgggcca tcgagccgct gcagattgca
     5281 attgatcacc agaaccaggt tgtccagccg ctcccggctg gcgatgttga tggtgccgag
     5341 cacctccggc tcgtcggact ctccgtcccc gatgaacgtc cagatcttgc cgccgttgtt
     5401 ggccttgagc ccgcggcttt ccagatattt ggcgaaccgc gcctggtaga tggcgctggg
     5461 cgtagagagc cccatgctgg ccgtgggcat ctgccagaac cacggcaggc ggcgcggatg
     5521 agggtaggag ggcagaccac caccgggcgc cagctcgcgg cggaagttgg tcaacgcctg
     5581 ctcggaaaga cgcccttcca gaaacgccct ggcatagacg ccgggcgagg tgtgggcctg
     5641 aaagtgcacc tgatcgccgc cgtaggattc ggacgcgcct cgaaagacgt gattgaaacc
     5701 cacttccatc atcgtcgcgg cggacaggta ggtggcgatg tgaccaccaa cccccgagcc
     5761 gctgtcgtag gcctgcagca ccatggcgac cgcgttccag cagatgatgt cgccgatctt
     5821 catctcgagc tcgatgttgc ccgggtaggc gggctgatcg cgcacgtcga tggtgttctt
     5881 gtacggggtg ttgagcgcgg cgtcggtcag aatcatgccg cgatgactga catgggcctg
     5941 cagccgccta agcagctcct tgacctcgtc ctcacccctg aaggcgagca cgtcgtcaat
     6001 ggcgtcgcac cattcctgct gctccgacag ccaatcgtcg gggttacgct ccgccatctc
     6061 cgtcaccgta cccaatgtct taggcaagcg gggaagctac gccatggggc aagcgcggta
     6121 aaccgctccg agaaacggtc gctgagccgg gaacgggatc ttgggcgaga atgcggccgg
     6181 acgagcacgc cagcctactg ctgaatcgta acgggctccc cgaggcgcag ggtgaccgcg
     6241 atcggtcgat gatcggagag ccggcagtcc agaacctcgt agtcgtcgat cgacagcccc
     6301 ggcgagacca gcacgtgatc cagagcaaac gcaggctgcc aggcgggata agtggggtgc
     6361 acctgatccg ccggcaccag atccgtttcc gaaagcggcg agttgaagag cagctgggca
     6421 agatgactgt tcatgtctcc catcaccacc acgtgctctt cggcgtcgat caactcgcgc
     6481 acatactcga gctgcacctg gcgggaacgc ccgcccaaag agaggtgcag aaccaccagc
     6541 gagaccgtcg actcgccgag cttgaagcgc atcaggatcg cgccgcgtcc cggaatggcg
     6601 ccgggcagct tgtggtcttc catgtgcacg ggcgcgattc gcgacagaac cccgttgccg
     6661 tgctgggcga acggacccag gtcacggttg cactgggtgt accagtaagg aaaacgcgcg
     6721 cgatcggcaa tgtactccac ctgattgatg aagccgctgc gaaggctgcc gccgtcgatt
     6781 tcctgaagcg cgaccagatc gtagtcgcgc accagatcgg cgatgtggcg gatattgatg
     6841 gcccgcgctt cgtgaggcag cacgtgcttc cagcccttgg tgacgtagtg gcggtacttg
     6901 gtggtccgaa tacccacctg aatgttgaag gtgaggagcc tgagcgaatg cgccgcctcc
     6961 tcgggagcca catcggactc caagtgcatg ttcacgaccg gggtgacagc cgtactacgg
     7021 ttctcgccgc gcctagacca agccaacatg gcggactgct ccatcgactt ggtcatgagt
     7081 ctagccttgc gctgcggatt ggactgcgta ctaactaaca tttcgttcga tcacgaggcc
     7141 ccagaagtgg ccgcgtcctc ttcctcaacg gacgccggct ccggggcggc gatgccacgc
     7201 gccgctcgct gctttgcgac aaggtggttg accacttcca gcatggcggc gttgctgccc
     7261 gccatccgac cgtccacgcg ataggtgcca tccaccacca gggcggggac cccggagacg
     7321 cgataagccc gggcatgggc cttggcctgc tcgatccggc tgcgaacccc gaaggagttg
     7381 aagacctgag cgaactgctg ggggttcacg ccggcgactt gctggaacag ggcggcaatg
     7441 gcgccaggtt cgcgcaggtc ccggccctct tcgtgcaccg cccgaaagat ggaatcgtgc
     7501 acggcgccgc cgagatccag gatctgggca gtgtagaacg ctttggcgag ggtttcccac
     7561 tcggcgctga aaatcgcggg aacgcgcctg aaacccacgt cctcgggctg gttctctcgc
     7621 caggcttcca acgccgggtc gaaggtcttg cagtgtatgc aggcgtagga gaatacctcc
     7681 accacctcta ccctggcggc gtctgccgtg gccacgctca ccggcagccg ctgatagtga
     7741 acgccctctt gccattcctg ggcaaaggcg gggcccggtg c
//
LOCUS       DBNHKV010000046         6219 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1848565, whole genome shotgun sequence.
ACCESSION   DBNHKV010000046 DBNHKV010000000
VERSION     DBNHKV010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1848565"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..525)
                     /locus_tag="AC16PP_01370"
     CDS             complement(274..525)
                     /locus_tag="AC16PP_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480300.1"
                     /translation="MVAMMLIVTGVLGTMTHMMPGCTRRCFAGPLAGTSQRRERRRQH
                     KRYQQMRNKGIHEAHERRLSRDPPLHNAYTVPLPTGSSD"
     gene            853..2160
                     /locus_tag="AC16PP_01375"
     CDS             853..2160
                     /locus_tag="AC16PP_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR6480301.1"
                     /translation="MKRRMMRTWLVASLAVAGVAQGSEDGGDLLRTGLTLELALERAV
                     RTNPNLAGIQSRYEAMAAIPSQVGTLPDPVLSFGAVNFPTDTFHVGQEPMTQLQVGIS
                     QTIPFPGKRALREAASSHEAEAARSSVAETRLRLIRDVSFNWWTLHYLDRAQEIVQRN
                     QELLRQFVQIAKTRYEVGGGLQQDVLLAQLELSKLLDQAIQLNGARDVEAARLNRLLD
                     QPANQPIELPRTPVGVFPDLVAQQELYQRGAESRPLLKRLRSEIDAAESRLELARKDY
                     YPDFTVGAAYGARSGENPMPGGGDRTDLLTLRVSVNLPIYQSRKRAQAVSQRRSEMDR
                     HRYALQDEVNAMRAEISQAVADYRRAREQLVLLDSGIIPQARQTVQSMLAGYQVGEVD
                     FLNLVRSQVTLYNYETLYWKAVTLANQAVARLSAAVGEEVRGG"
     gene            2153..3496
                     /locus_tag="AC16PP_01380"
     CDS             2153..3496
                     /locus_tag="AC16PP_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6480302.1"
                     /translation="MGKKFLLLVIVALAGGVGAGYFFAELSAGKPSETAPLEPEGRAR
                     EPIFYRNPMNPSVTSAVPAKDSMGMDYIPVYADEEAAGPAGTVSIDPIVVQNIGVRTA
                     RAQRAALSHTVRALGRIDFDERSIARLHPKVEGWVEEMYVDTTGAQVSEDTMLLSVYS
                     PRLVASQQEYVLALRNYESLKDSPIPDISRGAADLVASSRARLELLDVPEHQIVELEK
                     SRVPQKSLHIHSPAEGVVLKIGARQGQHVTPMTELYVIADLSRVWVYADVFEHDLPWV
                     RVGDAVEMTLGALPGETFGGLLDYIYPYAEAKTRTVKARLVFDNPDRLLRPEMFADVL
                     IRTSTEPNALVIPAEAVVRSGLTNQVFVVRAPGRFEPRVVELGVESEGRVSVLEGLAE
                     GEEVVTSGQFLIDSESKLREAAAKMMARPGDEASSPDGVGDHDHHHGGHDRGASP"
     gene            3493..>6219
                     /locus_tag="AC16PP_01385"
     CDS             3493..>6219
                     /locus_tag="AC16PP_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6480303.1"
                     /translation="MIARIIDGAIGERLIVLLGALILAGAGTWAYLTVPVDAIPDLSD
                     VQVIVFTDYPGQAPQVVEDQVTYPLTTAMLAVPKARVVRGYSFFGLSFVYVIFEDGTD
                     MYWARSRVLESLNYVSGRLPAGVNPALGPDATGVGWIYEYALVDRSGRHDLAQLRSLQ
                     DWYLRYPLQTVPGVAEVASVGGFVKQYQVEVDPNALMALGIPLSKIKQAIQRSNNDVG
                     GRLLEISETEYMVRGLGYIRSLEDLGSIPVAADPGGTPIRLGDVARIQIGPELRRGLV
                     DLDGDGEVVGGIVVMRYGENARATIEGVRQRLDELRAGLPEGVEIVTVYDRGDLIDRA
                     VDTLSRSLLIEIVIVSLVVIVFLAHARSAMVAVLILPLGVLIAFLLMRWHGLNANIMS
                     LGGIAITIGAMVDGAIVMVENAHKHMARDAERKGAELSSAERWQAVGAAAREVGPALF
                     FSLLVITVAFLPVFSMQAQEGRLFSPLAFTATYAMAGAAILAITLVPVLMGYLVRGRI
                     RPEESNPINRWLHRLHTPLLGLAMRWRWATVVMAGLLLALTWVPFSRLGSEFMPPLDE
                     GDILYMPTTFPGVSISKARELLQQTDRILREFPEVGQVFGKAGRAETATDPAPLAMFE
                     TTIRLKPRREWPDPTKTTRELIDEMDRELKLPGLANSWTMPIKARIDMLSTGIKTPVG
                     IKVSGPDLEVLQRLSQEIEIVMSSMPETLSAFGDRAVGGYYMDSDIDRSQAARYGLTV
                     GDVQDVIQSAIGGMNVTATVEGLERYPVNVRYPRELRDTPDALRRVLIATPTGVQIPL
                     EAVADISMNRGAPVIKSENGRPNAWIYVDPRTSDIGGFVAKAQRLVAQQVAIPAGYTI
                     RWSGQYEYMERARTRLQILVPVTLLAIFLLLYGHFRNVLAPAVVMLSIPFGLI"
BASE COUNT         1062 a         1906 c         2087 g         1164 t
ORIGIN      
        1 tagcgcacgg caaacagatt gatcagcgat gctcccacgt ccatgaccga atcgaccagt
       61 gaggccagga cgctcaccga atcggtggcg aaccaggcgg tcagtttcgc cgcgatgagc
      121 accgtggctg tagccacgga tgcgtaggtc gcaaagcgca gaagctgttc ggtggtcatg
      181 gcgggcatgg ccaggcagat cgggtggccg gagatgttac accacgaccg gttgcgacgg
      241 ggcctcgggc aggtgttttg accatcctct tgctcaatca gaggaaccag ttggcaacgg
      301 cacggtgtag gcgttgtgga gcgggggatc tcgcgatagc cttcgctcat gagcctcgtg
      361 aatgcccttg ttgcgcatct gctggtagcg cttatgctga cgacgccgct cacggcgttg
      421 gctggttcct gccagggggc cggcgaagca cctgcgcgtg cagccgggca tcatgtgggt
      481 catggtcccg agcacgccgg ttacgatgag catcatggcg accatgggga ggtgtccgtc
      541 gaagacgggg ccgcggactg tgattgctgc gacgagtgtc tccaggagtg tgccgcctcg
      601 ggatcgacgt cctttctcgc cgggagtgct ctggaagcgc cactgcgatc cggtccgaaa
      661 cggttcgtca ccgactggct gagcggtcac gtccgcgcgc ctcccacgtc cttattccgt
      721 cctccaatcc tcgactgctg atcgagcggc gctgctggcg ccccagttcc catggcccgt
      781 tgcggtcttc tgggagtcgg catggtgccg ctcatttctc ggtcatcagt gaactgaacg
      841 agggtgtggc ggatgaaacg acgaatgatg aggacgtggc tggtcgcgtc cctggcggtt
      901 gcgggtgttg cccaaggctc cgaggacggc ggcgatctgc tccggacggg tttgacgctg
      961 gagctggccc tggagagagc ggtgcgcacg aacccgaacc tggcgggtat tcagtcgcgc
     1021 tatgaagcga tggctgcgat accctcccag gtgggaactc tccctgatcc ggtgctgagc
     1081 ttcggtgccg tgaacttccc cacggacact ttccacgtgg gccaggagcc catgacccag
     1141 ctgcaggtgg ggatcagcca gaccataccg tttccaggca aacgggcgct gcgcgaagcg
     1201 gcttccagcc acgaggcgga ggccgctcgc agcagcgtag ccgagacccg gctgcggctg
     1261 atcagggacg tgtccttcaa ctggtggacc ctgcactatc tcgatcgcgc ccaggagatc
     1321 gttcagcgga accaggaact gctgcgccag tttgtccaga ttgccaagac ccgctacgag
     1381 gtgggcggtg ggctgcagca ggatgtcctg ctggcgcagc tggaactttc aaagctgctc
     1441 gaccaggcga tccagctcaa tggcgcccgc gacgtggaag ctgccaggct gaaccgtctg
     1501 ctggaccagc cggccaacca acccatcgag ctgcccagga cgcccgtcgg cgtctttccg
     1561 gatctcgtcg cgcagcagga gctgtaccag cgcggggcgg aatcgcgtcc gctgctcaag
     1621 cgcctgcgca gcgagatcga cgcggcggaa tccaggctcg agcttgcgcg caaagattac
     1681 tatcccgatt tcaccgtggg cgcggcgtac ggcgctcgat ccggcgagaa cccgatgcct
     1741 gggggtggcg acagaacgga tctgttgacc ctcagggtga gcgtcaatct gcccatctac
     1801 cagagccgga agcgcgctca ggcagtcagc cagcgccgca gcgagatgga tcgacaccgc
     1861 tacgcgctgc aggacgaggt caatgccatg cgggcggaga tttcccaagc ggtcgccgac
     1921 taccggcgcg cccgcgagca gctggtgctg ctcgactcgg gaatcattcc tcaggcgcgc
     1981 cagacggtgc agtcgatgct ggccggttat caggtcggcg aggtggattt tctgaatctc
     2041 gtgcgcagcc aggtcacgct ctacaactac gaaacgctgt actggaaagc ggtcactctg
     2101 gcgaatcagg ccgtcgcgcg gttgagcgcc gcggtgggcg aggaggtgcg aggtgggtaa
     2161 gaaatttctg ctgctggtga ttgtggcgct cgccggcggg gtgggcgcgg gctacttctt
     2221 cgcggagctg tcggcgggca aaccgagcga aacggcaccg ctggagccgg aggggagggc
     2281 gcgagaaccc atcttctatc gtaaccccat gaacccgtcc gtcacctctg cggtcccggc
     2341 gaaagactcc atgggtatgg actacatccc ggtttacgcg gacgaggagg ctgccggccc
     2401 ggcggggacg gtttccatcg atcccatcgt cgtgcagaac atcggcgtca gaacggctcg
     2461 cgctcagcgc gccgcgctca gccacaccgt gcgcgcgtta ggccgtatcg acttcgacga
     2521 gcggagtatt gccaggctgc accccaaggt cgaaggttgg gtcgaggaaa tgtacgtgga
     2581 caccaccggt gctcaggtca gtgaggacac catgctgttg agcgtgtact cgccgcgcct
     2641 ggtggccagc cagcaggaat acgtgcttgc gctcaggaac tacgagtcgc tcaaggacag
     2701 ccccattccc gacatcagcc gcggcgcggc cgatctcgtc gcgagttccc gtgcccggct
     2761 cgagctgctg gacgtgccgg agcatcagat cgtcgagctg gaaaaatccc gcgtcccgca
     2821 gaagtcgctg cacattcact cgcctgctga aggcgtggtc ctgaagatcg gggcccgcca
     2881 gggtcagcac gtgacgccca tgaccgagct ctacgtgatt gccgatctct cccgggtgtg
     2941 ggtttacgcc gacgtgttcg agcacgatct gccctgggtt cgggtgggcg atgcggtcga
     3001 gatgacgctg ggagcgctgc cgggagagac attcgggggg ttgctggact acatataccc
     3061 ctatgcggaa gcgaagaccc gaacggtcaa ggcgcgcctg gttttcgaca acccggaccg
     3121 gcttctgaga ccggagatgt tcgccgacgt gctcatccgt acgtcgacgg agcccaacgc
     3181 gctggtcatt cccgcggagg cggtcgtccg ctcgggtctg accaatcagg tgttcgtcgt
     3241 gcgtgcgccc ggccgctttg agccgcgggt ggtcgagctc ggggtggagt ccgagggtcg
     3301 agtcagtgtt ctcgaagggc ttgccgaggg cgaggaagtg gtcacgtccg gccagtttct
     3361 gatcgattcg gaatccaagc tccgcgaggc tgctgcgaag atgatggcgc gcccgggtga
     3421 tgaagcgtcg tcgcccgacg gagtcggcga tcacgaccat caccacggcg ggcatgaccg
     3481 tggagcgtcg ccgtgatcgc gcgcatcatc gatggggcga tcggcgagcg tttaatcgtt
     3541 ctgctcggag cgctcatcct cgccggtgcg ggtacatggg cctacctcac cgtgcccgtg
     3601 gatgccattc ccgacctgtc cgacgtgcag gtgatcgtgt ttaccgacta tcccggacag
     3661 gcgccccagg tcgtggaaga tcaggtgacc tacccgctca ccacagcgat gctcgccgtt
     3721 cccaaggccc gcgtggtccg cggatactct ttcttcggct tgtctttcgt ctacgtgata
     3781 ttcgaagacg gcaccgacat gtactgggcg agatccagag ttctggagtc gctcaactac
     3841 gtcagcggcc gcctgccggc cggcgtcaac cccgcgcttg gccctgatgc gacgggcgtg
     3901 ggctggatat acgagtacgc cctggtggat cggtccggcc gccacgatct ggcgcagctc
     3961 aggtctcttc aggactggta tctgcgctat ccgctgcaga ccgtgcctgg cgttgcggag
     4021 gttgcttccg ttggcgggtt tgtcaagcag taccaggtgg aggtggaccc caacgcgctc
     4081 atggcccttg gtatcccgct gtcgaaaatc aagcaggcca ttcaacgttc gaacaacgat
     4141 gtgggcgggc ggctattgga aatctccgag accgagtaca tggtgcgggg cctgggctac
     4201 attcgatctc tggaggacct cgggtcgata ccggtggccg cggatccggg cggtacgccc
     4261 atccgccttg gggatgtggc gcggattcag atagggcccg agctcaggcg tgggctggtg
     4321 gatctggatg gagacggcga agtggtgggc ggcatcgtgg tgatgcgtta tggggaaaat
     4381 gcgcgcgcca ccatcgaagg cgtgcgccaa cggctggacg agctcagggc cggcctcccg
     4441 gaaggtgtgg agatcgtgac ggtctatgac cgcggggatc ttatcgatcg ggcggtggat
     4501 accctcagcc ggtcgctgct catcgagatc gtcatcgtca gcttggtggt gatcgtcttt
     4561 ctggcccatg caaggtcggc gatggtggcc gtcttgatcc tcccgctggg cgtgctcatt
     4621 gctttcctgc tgatgcgctg gcatggcctg aacgccaaca tcatgtccct tggagggatc
     4681 gcgatcacca tcggtgccat ggtggatggg gcgatcgtca tggttgagaa cgcgcacaaa
     4741 cacatggccc gcgacgccga gcgcaagggt gccgagctct catcggccga gcgatggcaa
     4801 gccgtgggcg cagcggctcg cgaggttggt ccggccctgt tcttctcgct gctcgtgatc
     4861 accgtggcct ttctgccggt attctcgatg caggcccagg aagggcggtt attcagcccg
     4921 ctggctttca cggcgaccta cgcgatggcg ggcgcagcga tcctggccat tacgctggta
     4981 cccgtgctca tgggctatct ggtcagaggg cgtattcggc cggaggagtc caaccccatc
     5041 aaccgatggc tgcaccgtct ccacacgccg ctgctgggtt tggccatgcg ctggcgctgg
     5101 gcgacggtgg tgatggccgg cttgctgctg gcgctcacgt gggtgccgtt ctcgcgtctc
     5161 gggtcggaat tcatgccgcc gctggatgag ggggacattc tctacatgcc caccacgttt
     5221 cccggagtct ccatctccaa ggccagggaa ctgctccagc agaccgaccg tatccttcgc
     5281 gagtttcccg aggtcgggca ggtcttcggg aaagccggtc gtgcggagac ggccacggat
     5341 ccagcgcccc tggcgatgtt cgaaaccacg attcggctca agccgcgccg cgagtggccc
     5401 gatcccacca agaccacgcg agaactcatc gacgagatgg accgcgagct gaagctgccc
     5461 ggcctggcca acagctggac catgcccata aaggcgagga tcgacatgct gtccaccggc
     5521 atcaaaaccc ctgtgggcat caaggtatcc ggccccgacc tggaagtgtt gcagcgcctc
     5581 tcccaggaga tcgagatcgt catgagttcc atgccggaga ccctttcagc ctttggcgat
     5641 cgtgccgtgg gcggctacta catggactcc gacatcgacc ggtctcaagc cgcccgctac
     5701 gggttgacgg tgggcgacgt gcaggacgtc atccagagcg caataggagg catgaacgtc
     5761 acggcgacgg tggagggcct ggagcgctac ccggtgaacg tgcgttaccc tcgggagctc
     5821 cgcgatacgc cggacgcgct gcgccgggtg ctcatcgcca cgcccaccgg ggtgcagatc
     5881 ccgctcgagg cggtggcgga tatctccatg aaccggggcg cgccggtgat caagagcgaa
     5941 aatggacgtc ccaacgcctg gatttacgtg gacccgagga cctccgacat cggcggcttc
     6001 gtcgctaagg cccaacggct ggtagcccag caggtcgcga tcccggccgg ctacaccatc
     6061 cgctggtccg gacagtacga gtacatggag cgagcgcgta cccgcctgca gattcttgtc
     6121 ccggtgaccc tgctggccat ttttctcctt ctctacggtc acttccgcaa cgtgctcgca
     6181 ccggccgtgg tgatgctttc catcccgttc ggactcatc
//
LOCUS       DBNHKV010000047         6162 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_149255, whole genome shotgun sequence.
ACCESSION   DBNHKV010000047 DBNHKV010000000
VERSION     DBNHKV010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_149255"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="AC16PP_01390"
     CDS             <1..1247
                     /locus_tag="AC16PP_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480304.1"
                     /translation="TRMLAVTGIAEDPEVEDAWSNAQSLHQDPNQSLATIVAGYRRVV
                     NLDPDHEGARAAIAGLASQWKSSIEDSLAAENLEQAGTKLEESLTVFPEDPSLVELTE
                     QLTNRQHAESLLASTQALLRSHGLSDIPSATAAIQAYQEVLRLAPDHAVATEELNSLA
                     QHYASLAAEAADTGEVDAAISFLDRASAANSELRELDLVRSKIQQATTLQAAIIELLQ
                     QASAYRAAGALINPPGENAAELYHRVLATYPENVIATQGLDEVVSQLRGTANQLLAAG
                     DLGALQELVDRSSAVGLDPVAVNEMKSSLETEIARRATVAERLDQARGLLAEGFITEP
                     QGRNVVALLREVERLDPGNEPAKRMLKESAERLASVAQEAYEVGMVDDAKHYLELALT
                     VTPDVAAWRELRATWEKSVASN"
     gene            1231..2364
                     /locus_tag="AC16PP_01395"
     CDS             1231..2364
                     /locus_tag="AC16PP_01395"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR6480305.1"
                     /translation="MLQVTDLQYFISFHDDEFPLEDSLTIGRHLDNDVIVAGEDVLDY
                     HLRLEPTPRGPKVHPLGEASLILNGHHYSESRGLVAGDELVVGQNVLGVGVRRLHPAE
                     AESWSLHGDAAHGVHEMVGDLSVGRGEDSGLVVADDHVSREHALFTLADDVVWVRDLD
                     SANGTFVNGERVSGGCRLYHGDMIAFDQFEFQLIGQGADLTPVRAREGMQEGAMIKES
                     GPARSDTTEIAVVANADAVAELAPPAREKGTFLLGAAEPVLGITYRLRMGKTSLGRDD
                     ACDVVVKDRTVSSRHAEVVLRPEGATITNLMATNGTRVNGREIQSAQLADGDIIGLGR
                     VRFVYRSVTEGRRAARLRPLHRWLIAGAAIVAAVLAGLLIDTL"
     gene            2388..3290
                     /locus_tag="AC16PP_01400"
     CDS             2388..3290
                     /locus_tag="AC16PP_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6480306.1"
                     /translation="MGVHIPYRRDLEFTYGEVAEIAPGIRRVIAENPSPFTLYGTGTY
                     ILGTGNVAVIDPGPADPAHIAAIVAGLQGETISHVLVTHTHMDHSPGCALLREHTDAP
                     TYAFGPHGAGKLEEGVPVEEGGDMEFVPDVVVRHGDVVEGADWSVECVYTPGHTSNHM
                     CFQLREQKALFTGDHVMGWSTSIISPPDGDMFAYMKSLDLLLERDDAVYWPTHGPCID
                     APKTHVRAFIEHRREREEQILKCVSRGVGQIRDMVPIMYVDTPEFMYPAAARSVLAAV
                     EGLVARGDLACDGAVSLDGNYRLT"
     gene            complement(3287..5488)
                     /locus_tag="AC16PP_01405"
     CDS             complement(3287..5488)
                     /locus_tag="AC16PP_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6480307.1"
                     /translation="MKIFSRIKGPRLGTKIMLLGLTLLIVPWFSYRQLVEMERLLIQG
                     QSQAQLLTAEGISTLFNGREDLFNDLPVAVEDFENLYANPLQAAVRLDGRLDDWGSEI
                     PGKLMHFGSEKTGADADFELVLGERGGQLHVFMRVTDPDLIYRNPDYLRLDNADHVRL
                     SFIRADGEDGRILLTLPAPGLVTAYEMDADWRFAESGNPTNAVQGFMEPVDGGLQLEF
                     RLPLQLLGSSRYFGIAYVDVDDPALREIVHVTQTLPKAGLESFNLVVLRSPEVLNIIQ
                     GLGYSGARILVIDAQQRVRAETGSNRPIEETDPASPTFVLLRDGFEQVRPFLHWVSTG
                     EDYLPVKSRPEDSEGTADVAITTSLTGDPIALRRALSENNEIIIAAHPIVSRDAVIGT
                     VVVEQNIDDILSFQRSALEQVILLSIASLFAVFIALLAFAGRLAWRIRNLRQEASSAI
                     DQYGRLKISELHNEMKAGDEIGDLARSVSNMLSKLHQHNNFLENMPRTLRHEINNPLN
                     TLSTSLQNLAEENPEIQDSKYMESAKRGVNRIGSIVQNLADAANLEESLEAEELEVVD
                     IAQLLENYVANCRITHPTCEFVFRGPNRPVLSRVSDFRIEQLLDKIIDNAIDFHRSNS
                     PIVVQLDAYRDHLQITVANRGPILPAKAEKSLFDSMVSHRGQQNRLHFGLGLYVVRVI
                     ADHHGGFVRALNLADGSGVAIVVQLPTVETTEGTPGRQRDAEDIEPGVAAS"
     gene            complement(5504..>6162)
                     /locus_tag="AC16PP_01410"
     CDS             complement(5504..>6162)
                     /locus_tag="AC16PP_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6480308.1"
                     /translation="RANYDDAITMKGYKVSAYGSRQEALEGFDQALPDLVILDIILGE
                     EVDAGFQLCRDLLARAPSLPVIFLTERINEIDKISGLRLGAWDYLPKPISLDYLAERI
                     SSLLRINEVRAEQQETESATAKRIGDLMLDQDALLVSWKGHRIDLSGTEFRMLAKLVR
                     MPGHAVSYETLMNATMQSLVTNNTINTHMRNIRKKFEKVDKDFSSIKSEYGFGYRWSA
                     "
BASE COUNT         1057 a         1937 c         2062 g         1106 t
ORIGIN      
        1 tcacccgcat gcttgccgtc acgggcatcg ccgaggatcc cgaggtggaa gatgcctgga
       61 gcaacgccca gtctctgcac caggatccca atcagagcct ggcgaccatc gttgccggtt
      121 accggcgggt ggtgaatctg gatcccgacc acgagggcgc tcgcgccgcc attgccgggc
      181 tggccagcca gtggaagagc agcatcgagg attctcttgc ggcggagaat ctcgagcagg
      241 cgggtacgaa gctcgaagag tcgctgacgg tttttcccga ggatccttcg ctcgtcgagc
      301 tcaccgagca gctgacgaac cggcagcacg cggaaagcct gctcgccagc acccaggctc
      361 tgctgcgcag tcacgggctc tccgacattc cctctgcaac ggctgccatc caggcctatc
      421 aggaggttct gcggctcgcg cccgaccacg ccgttgccac ggaggagctc aactcccttg
      481 cacagcacta tgcttcgctg gccgcggaag cggcggatac gggcgaggtg gatgcggcga
      541 tcagcttcct cgatcgggcg tcagccgcca actcggagct gcgtgagctg gacctggttc
      601 gatcgaagat tcagcaggcg acgacgctcc aggcagccat catcgagctg ctgcagcagg
      661 ccagcgccta tcgggcggcc ggcgcgctca tcaacccgcc gggcgagaac gcggccgagc
      721 tctatcatcg ggtgctcgca acgtaccccg agaacgtcat cgccacacag gggctcgacg
      781 aggtggtttc ccagctgcgc gggacggcca atcagctgct tgccgcgggt gacctggggg
      841 cgctgcagga gctcgtcgac cgttccagcg ccgtaggcct cgacccggta gccgtcaacg
      901 agatgaagag cagcctcgaa acggagatcg ctcggcgcgc gaccgtcgcc gagcggctcg
      961 atcaagcgcg cgggctgctg gcggagggat tcatcacgga gccccaggga cgcaacgtgg
     1021 tcgctctgct gcgcgaagtc gagcgtctcg acccgggcaa cgagccggct aaacgcatgc
     1081 tcaaggagtc cgccgagcgt cttgccagcg tggcgcagga agcttacgaa gtgggcatgg
     1141 tggacgacgc caagcactat ctcgagctgg cgctgacggt gacgccggac gtcgcggcct
     1201 ggcgagagct tcgagcgacg tgggaaaaga gtgttgcaag taactgatct gcaatatttt
     1261 atttcttttc atgacgacga atttccgctc gaagacagcc tgaccatagg gcgtcatctc
     1321 gacaacgacg tcatcgttgc cggtgaggac gtgctcgact accacctgcg cctggaaccc
     1381 accccacggg ggccgaaagt ccacccgctg ggggaggcaa gcctgattct caacggtcat
     1441 cattactccg aatcccgggg cctggtggcc ggcgacgagc tggtcgtcgg ccagaacgtg
     1501 ctcggggtag gggttaggcg cctgcatccg gcggaagccg agagctggtc gctccacggt
     1561 gatgccgcac acggcgttca cgagatggtc ggagacctga gcgtgggacg cggcgaggac
     1621 agcggcctgg tcgtcgccga cgaccacgtc tcccgcgagc atgcgctctt cacgctggcc
     1681 gatgacgtgg tgtgggttcg ggatctcgat tcggccaacg gcaccttcgt caacggcgag
     1741 cgcgtctcgg gaggctgccg tctgtatcac ggcgacatga tcgcgttcga tcagttcgag
     1801 tttcagctca tcgggcaggg ggcggatctg acccccgtgc gtgcgcgcga aggcatgcag
     1861 gaaggggcca tgatcaagga gtccgggccg gctcgctcgg ataccaccga gattgccgtc
     1921 gttgccaacg ccgatgccgt agccgagctg gcgccgcccg cccgtgaaaa ggggacgttt
     1981 ctgctgggcg cggcagagcc cgtccttggg atcacctacc gtctgcgcat gggcaagacg
     2041 tcgctcgggc gcgacgatgc ctgcgatgtc gtggtcaagg atcgcacggt ctcgtcgcgc
     2101 cacgcggagg tcgtcctgcg tccggagggc gccaccatca ccaacctgat ggcgaccaac
     2161 ggaacccggg tgaacgggcg cgagatccaa agcgcccagc tcgccgacgg cgacatcatc
     2221 gggctcggga gggtgcgttt cgtctatcgc agcgtcacgg agggcaggcg cgctgcgcgc
     2281 ctgcgtccgc tgcaccgttg gctgatagcg ggcgccgcca tcgttgcggc cgtcctggca
     2341 ggtcttctca tcgacaccct ctagcgcgat ggattatgct tcgcctcatg ggcgtacaca
     2401 taccgtatcg acgcgatctg gagttcacct acggtgaggt cgccgagatc gcgccgggaa
     2461 tccgccgcgt catcgccgag aatcccagcc cgttcaccct ctacggtacc ggcacgtaca
     2521 ttctgggcac cggcaacgtt gcggtgatcg atcccgggcc ggccgatccg gcccacatcg
     2581 cggccatcgt cgccggcctt caaggcgaaa ccatttctca cgtgctggtg acccacaccc
     2641 acatggatca ctccccgggc tgcgcgctgc tgcgtgagca caccgatgcg ccaacttatg
     2701 ccttcgggcc tcacggcgcc ggcaagctcg aggagggcgt ccccgtggag gagggcggcg
     2761 acatggagtt cgtgcccgac gtggtggtcc gtcacggtga cgtcgtcgag ggcgccgact
     2821 ggtccgtgga gtgcgtctac acgcctggcc acacctccaa tcacatgtgt ttccagctgc
     2881 gcgagcagaa agctcttttc accggcgacc atgtcatggg ctggtcgacg agcatcatct
     2941 cgccaccgga tggcgacatg tttgcctaca tgaaaagcct cgatctgctg ctcgagcgcg
     3001 atgacgccgt ctactggccc acccacgggc cctgcatcga tgcgccgaaa acccacgttc
     3061 gggccttcat cgagcatcgc agggagcggg aggagcagat tctgaagtgc gtgagccggg
     3121 gcgtcgggca gattcgcgac atggtgccca tcatgtacgt cgacactcct gagttcatgt
     3181 atccggccgc agcccgcagc gttctggctg cggtggaagg gctggtggcc cggggcgacc
     3241 tcgcctgcga cggcgcggtc tctctcgacg gcaactaccg actgacttag ctcgccgcta
     3301 ccccgggctc gatatcctcc gcgtcccgct gacgacccgg cgttccctcc gtcgtctcga
     3361 ctgtgggcag ctgaacgacg atggcgactc cggagccatc ggccaggttg agcgcccgca
     3421 cgaacccgcc gtggtggtcg gcaatgaccc ggaccacgta caggccgagg ccgaagtgca
     3481 gccggttctg ctgtccccgg tgactgacca tggagtcgaa aagcgacttt tccgccttgg
     3541 cgggaaggat ggggccgcgg ttagccaccg tgatctgcag atggtcacga taggcatcga
     3601 gctgaaccac gatggggctg ttggagcggt gaaagtcgat ggcgttgtcg atgatcttgt
     3661 cgagcagctg ctcgatgcgg aagtcggaca cccggctgag caccggacga ttgggcccgc
     3721 gaaacacgaa ctcgcacgtc gggtgggtga tgcggcagtt cgccacgtag ttttccagga
     3781 gctgagcgat gtccaccact tcgagctcct cggcttccag cgactcttcg agattcgcag
     3841 cgtccgccag attctgcacg atggagccga tgcggttgac gccgcgcttg gcgctctcca
     3901 tgtacttgct gtcctgtatc tcggggttct cctcggccag gttctgcagg gacgtgctga
     3961 gggtgttcag cgggttgttg atctcgtgcc ggagcgtgcg gggcatgttc tccaggaagt
     4021 tgttgtgctg gtgcagcttg gacagcatgt tggaaacgct gcgcgcgaga tcgccgatct
     4081 cgtcgccggc cttcatctcg ttgtgcagct cgctgatctt cagacgcccg tactgatcga
     4141 tggccgagct tgcctcctga cgcaggtttc ggatgcgcca tgcgaggcgg ccggcaaagg
     4201 cgagcagggc gatgaagacg gcaaacaggc tggcaatgga caacaggatc acctgctcca
     4261 gggccgaccg ctggaaggag aggatgtcgt cgatgttctg ctccaccacc acggtgccga
     4321 tgacggcgtc tctggagacg atggggtgcg cggcgatgat gatctcgttg ttctcgctca
     4381 gcgcccggcg cagagcgatg gggtcgccgg tgagcgaggt ggtgatcgcc acgtccgccg
     4441 tgccttcgga atcctccggg cggctcttca cgggcaggta gtcctcgccg gtggagaccc
     4501 agtgcagaaa gggccgcacc tgctcgaagc cgtcgcgcag caggacgaac gtcgggctcg
     4561 caggatcggt ctcctcgatg gggcggttgc tgccggtctc cgcgcgaacc cgctgctggg
     4621 cgtcgatgac caggatgcgg gcgccggaat accccaggcc ctgaatgatg ttcagcacct
     4681 cgggcgagcg cagcaccacc aggttgaagc tctccagccc cgccttgggc agggtctggg
     4741 tgacgtggac gatctcgcgc agggccgggt cgtccacgtc cacgtaggcg atgccgaagt
     4801 agcgtgacga gcccagaagc tgcagcggca ggcggaactc cagctgcagc ccgccatcca
     4861 ccggctccat gaatccctga accgcgttcg tcgggttgcc cgactcggcg aagcgccagt
     4921 cggcgtccat ctcgtaggcg gtgacgagcc cgggagctgg caaagtgagc agaatccgtc
     4981 cgtcctcgcc atcggcgcgg atgaagctca ggcgaacgtg gtcggcgttg tcgagccgca
     5041 ggtaatcggg gttgcggtag atcagatccg ggtcggtgac gcgcatgaag acgtgcagct
     5101 gcccgccgcg ctcaccgagc accagctcga agtccgcatc ggcccccgtc ttctcgctgc
     5161 cgaagtgcat gagcttgccg gggatctcgc tgccccagtc gtcaagccgg ccgtccaggc
     5221 gcacggccgc ctggaggggg ttggcgtaaa ggttttcgaa gtcttccacc gcgacgggca
     5281 gatcgttgaa cagatcctcc cggccgttga agagggtgga gatgccttcg gcggtgagca
     5341 gctgggcctg tgactggccc tgaatgagca gccgctccat ctccaccagc tggcggtagc
     5401 tgaaccaggg aacgatgagc agggtgagcc cgagcagcat gatcttggtg ccgagccggg
     5461 gacccttgat gcgactgaag atcttcatgg gcgttggcga gcgtcaggcc gaccagcgat
     5521 atccgaaccc gtactcgctc ttgatgctcg agaagtcctt gtcgaccttt tcgaacttct
     5581 tgcggatgtt gcgcatgtgg gtgttgatgg tgttgttggt gacgaggctc tgcatggtcg
     5641 cgttcatgag cgtttcgtag ctcacggcat ggcccggcat gcgaacgagc tttgccagca
     5701 tccggaactc ggtgccggac agatcgatgc ggtgaccttt ccaggacacc agcagcgcgt
     5761 cctggtcgag catgaggtcg ccgatccgct ttgccgtggc gctttccgtc tcctgctgct
     5821 cggcccgcac ctcgttgatg cgcagcagcg aggagatccg ctcggccagg tagtccaggc
     5881 tgatgggctt gggcaggtag tcccaggcgc ccagacgaag ccccgagatc ttgtcgatct
     5941 cgttgatccg ctcggtgagg aagatgacgg gcaacgatgg cgcgcgggcg agcaggtccc
     6001 ggcaaagctg aaagccagcg tccacctcct cgccgagaat gatgtccagg atgaccagat
     6061 cgggcagcgc ctgatcgaag ccctccagtg cttcctgccg gctcccgtag gcggaaacct
     6121 tgtagccctt catggtgatc gcgtcgtcgt agttggcccg ct
//
LOCUS       DBNHKV010000048         2039 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_787349, whole genome shotgun sequence.
ACCESSION   DBNHKV010000048 DBNHKV010000000
VERSION     DBNHKV010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_787349"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /gene="cofD"
                     /locus_tag="AC16PP_01415"
     CDS             complement(<1..899)
                     /gene="cofD"
                     /locus_tag="AC16PP_01415"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HIR6480309.1"
                     /translation="MSGYLAITGGVGGAKLGLGLAQILPPDELTFAVNTGDDFEHLGL
                     HIAPDVDTLTYTLAGLSNTETGWGRADETWDFMDALDGLGGETWFRLGDRDLALHVMR
                     THLLSAGACLTEVTDHICRALGVAHRVLPMSDDPVRTTVHTERGPLPFQHYFVRERCE
                     PAVTGFEFAGIGQARLNPAITSRLDGLRGIIICPSNPFVSIDPMLNLRGARAALKESG
                     VPIVAVSPIVGGAAIKGPTAKMMRELNVPATAAQVAEHYGDLLCGLVLDEQDAHLEGT
                     LSTPTIVAQTVMVTLQDRVDLGRT"
     gene            complement(899..1693)
                     /gene="cofE"
                     /locus_tag="AC16PP_01420"
     CDS             complement(899..1693)
                     /gene="cofE"
                     /locus_tag="AC16PP_01420"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HIR6480310.1"
                     /translation="MDKPEATSRTASPGFSVLGIPGVPMVSPGDDLTAIIVDALRDAR
                     ITLEDGDIVCVAQKIVSKAEGRQIPLASVTPSEEARTLAEETDKDPRLVELILRESSE
                     VMRKKEGVLIVRHRLGLVGANAGIDQSNISHEDGEQALLLPEDPDRSAAELKSALDEA
                     SGARVGVIITDSANRPWRLGTVGIAIGCAGISVLDDHRGGVDLFDRELKVTLISRADG
                     IAGAATLVMGETTERIPVALVRGFPAEQSTDDARMVCRPLHEDLFR"
     gene            complement(1704..>2039)
                     /locus_tag="AC16PP_01425"
     CDS             complement(1704..>2039)
                     /locus_tag="AC16PP_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR6480311.1"
                     /translation="TVQLPPEGSAGKRAQDMLGEDVMVVTAFQNIAAHLLQEDVHIEC
                     DVLVAGNKKAARDKVIELVEQAGMKGYHAGPIENSAAAEALTSILIQINRRHDISHSG
                     IKIVGQSDH"
BASE COUNT          317 a          675 c          690 g          357 t
ORIGIN      
        1 gtgcggccga gatccacacg gtcctgcaac gttaccatga cggtctgggc cacgatagtc
       61 ggggtactca gcgtgccttc cagatgcgca tcctgctcgt cgagcacgag tccacagagc
      121 agatcgccgt agtgctcggc cacctgagcc gcggtcgccg ggacgttgag ctcgcgcatc
      181 atcttcgcgg tgggcccctt gatggcggct ccgccaacga tgggagagac cgccacgatg
      241 ggcacgccgc tttcttttag agcggcgcgg gcgccgcgca ggttcagcat ggggtcgatg
      301 gagacgaacg ggttggaggg gcagatgatg atgccgcgaa ggccgtccag gcgcgacgtg
      361 atcgccgggt tgagccgcgc ctgcccgatc ccggcgaact cgaacccggt caccgccggc
      421 tcgcaccgtt cccgcacgaa gtaatgctgg aaaggcaggg gcccccgttc agtgtgcacg
      481 gtcgtgcgca caggatcgtc ggacatcggc agcacccggt gggcgacgcc cagggctcgg
      541 cagatgtggt cggtgacttc ggtgaggcaa gcaccggccg acagcaggtg ggttcgcatc
      601 acgtgcagcg ccaggtcccg atcgccgaga cggaaccagg tctcaccgcc gaggccgtcc
      661 agggcatcca tgaaatccca ggtctcgtcg gcgcgccccc agccggtctc ggtgttgctc
      721 agccccgcca gggtataggt gagggtgtcg acgtccggcg cgatgtgcag acccaggtgc
      781 tcgaagtcat cgcccgtatt cacggcgaag gtgagctcgt ccggcggcag gatctgcgcg
      841 agaccgagcc ccagtttcgc cccgcccacg ccacccgtga tggcgagata cccggacatt
      901 atcgaaacag atcctcgtgc agaggtcggc agaccatgcg ggcgtcgtcg gtggattgct
      961 cggccgggaa gccgcgcacc agggccacgg gaattctctc cgtggtttcg cccatgacca
     1021 gggtggcggc gccggcgatg ccgtccgccc ggctgatgag ggtgaccttc agctcccggt
     1081 cgaacagatc gacgccgccg cgatgatcgt cgagcacgct gatgcccgcg cagccgatgg
     1141 ctatgcccac ggtgcccagc cgccaggggc gattggcgct gtcggtgatg atcacgccga
     1201 cccgggcgcc gctggcctcg tcgagggcag acttcagctc ggccgccgag cggtccgggt
     1261 cctcgggaag gaggagcgcc tgctcgccat cctcgtggga gatgttcgac tggtcgatcc
     1321 ccgcgtttgc tccgaccagg ccaaggcgat ggcgcacgat cagcacgccc tcttttttcc
     1381 gcatcacctc tgacgattcg cgcaggatca gctcgacgag ccgcgggtcc ttgtccgttt
     1441 cctccgccag cgtccgggcc tcctcgctcg gcgtcaccga cgccagaggt atctggcgtc
     1501 cctcggcctt ggagacgatc ttctgcgcaa cgcagacgat gtcgccgtct tccagggtga
     1561 tgcgggcatc gcgcagggcg tcgacgatga tggccgtcag gtcgtccccg gggctcacca
     1621 tgggcacgcc cgggatgccc agcacggaaa agccgggcga tgccgtcctg ctggtggctt
     1681 ccggcttgtc cattcgcgtt tggtcagtga tccgattgac cgacgatctt gatcccggag
     1741 tgggagatgt cgtggcgcct gttgatctga atcaggatcg aggtgagcgc ttcggccgcg
     1801 gcggaattct cgatgggccc cgcatggtag cccttcatgc cggcctgctc gaccagctcg
     1861 atgaccttgt cccgggcggc cttcttgttc cccgccacca gcacgtcgca ctcgatgtgc
     1921 acgtcttcct gaaggaggtg ggcggcgatg ttctgaaagg cggtaacgac catcacgtcc
     1981 tcacccagca tgtcctgggc ccgcttgccg gcgctgccct cgggcggaag ctgcacggt
//
LOCUS       DBNHKV010000049         7726 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1000210, whole genome shotgun sequence.
ACCESSION   DBNHKV010000049 DBNHKV010000000
VERSION     DBNHKV010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1000210"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1199)
                     /locus_tag="AC16PP_01430"
     CDS             complement(129..1199)
                     /locus_tag="AC16PP_01430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HIR6480312.1"
                     /translation="MRYRTLHEIVRQAQRNLDRDCRDYLLGGADTESSLRRNRHGLDS
                     WVFRPRILNDVSEVNLTIELLGAPMRIPVVLPPIGSVQLFEPGGGQTVAEAAHEFGIL
                     QILSSVCTPDFEAVAAAVPGPRIYQLYLMGDRVWLDDMIGRTIEAGYTGFCLTADTQI
                     YSRRERDILKGFVPPSGSQAGSRDFNYQARMTWETVAHIKSTFDIPLIVKGVNAGEDA
                     ARCVEAGVDVVYVSNHGGRQLDHTRACIDALPEVVEAVAGRVPVIVDGGFLRGADVVK
                     GLCLGADVVGMGRLEGLAMAAGGRGALVRALEIVEQEIRTTMALLGVSDLADLNPGLM
                     ERAQPLAESTVFSAFPLLEEGY"
     gene            1371..2576
                     /locus_tag="AC16PP_01435"
     CDS             1371..2576
                     /locus_tag="AC16PP_01435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6480313.1"
                     /translation="MTGMVIVGAGHAAGQAAASLRQEGYEGEITIIGDEPHIPYQRPP
                     LSKQYLAGEQGLERVYLRPEKFYADKEITLRMGVSVTAIDTQAHTVTTDDGETIGYEK
                     LLLATGSRPRILNVPGSDLAGIHYLRTIADVDGIRAELAEGRRLVIVGGGYIGLEVAS
                     VGIQAGLEVHVLEMEERILQRVTTPEMSDYYHQLHTGRGVHIHTSTMVSGFTGEGRVG
                     AVQCGDEEIAADIVVVGIGIIPNVEVAQAAGIECDNGIVVDEGCRTSADDVFAAGDCT
                     NHPNPLLHRRLRLESVPNAMEQARVAAANMCGADKVYESIPWFWSDQYELKLQMVGFS
                     ADGDTQVVRGDKAANQFAVFYLKDGAVVAADAVNSPKEFMVCKQLVGKPVDAARLGDP
                     DVELKSLLA"
     gene            complement(2781..3428)
                     /locus_tag="AC16PP_01440"
     CDS             complement(2781..3428)
                     /locus_tag="AC16PP_01440"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR6480314.1"
                     /translation="MHRAVERKGGRAALDALLPAARSGSDLAALPDRDYLAEMTRCVF
                     RSGFVWKIIENKWPGFEVAFRGFDVAGCAMLSDEDIEQLSADERIVRHAAKIRSVPAN
                     AAFILDVRREAGGFGRYLADWPEDEIVGLWDELKRRGSRLGGQTGRYFLRFVGKDTPI
                     LSRDVVKALIGQGVIDREPTSKKALARVQDAFNEWHAESGRELCQISRVLSLSVD"
     gene            3484..3861
                     /locus_tag="AC16PP_01445"
     CDS             3484..3861
                     /locus_tag="AC16PP_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cereblon family protein"
                     /protein_id="HIR6480315.1"
                     /translation="MVDVLDKTRQDLDPKLKEIVEKDERNFVYCGICSHVLTREVERI
                     EVSGSHDHHCTNPFGLRFHIGCFAEALGCDISGEPMAADTWFPGFRWRHASCAECHQH
                     LGWYFEAGERYFYGLILDRIQHD"
     gene            3854..4543
                     /locus_tag="AC16PP_01450"
     CDS             3854..4543
                     /locus_tag="AC16PP_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR6480316.1"
                     /translation="MTDLAAGNYRTLTFDCYGTLIDWENGILGYLQPLLESYDVHVLD
                     EWVLEFFAENEPALQAEGGSYRSVLARLLERLGTRCAFTPNDDALKGFADSIEYWQPF
                     ADTNAALRALSEHFDLAVVSNVDNELFSLSARQLGVEFAQVITADDVGAYKPARAMFD
                     EALRKVSGPVLHVAQSLYHDIAPAKALGLDTVWINRPSAAGSGAARFVEVEPTWTFGD
                     LSELVEAFDLD"
     gene            4596..5108
                     /locus_tag="AC16PP_01455"
     CDS             4596..5108
                     /locus_tag="AC16PP_01455"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIR6480317.1"
                     /translation="MSRPSLTSIQISKEYLHFNSAHFTLFSDREREDLHGHTFYVAGA
                     FDAEVGPDGLAFDYNLVKTKLKQLCDELDEKTLLPERSPHLRLSEENGYLVAEFAGER
                     MPFLPRDVLTLPVRNVTVEELAPFLLERLGSDPEIRTLELQRIELRVSSGPGQWAAAT
                     WTAGQEIREV"
     gene            5112..5807
                     /locus_tag="AC16PP_01460"
     CDS             5112..5807
                     /locus_tag="AC16PP_01460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6480318.1"
                     /translation="MKILLITGASAGIGLHTAQRFVAEGFAVVNLSRRRCPIDAVSQI
                     NCDLSVPGFIDNISNQLSGMLQEADRVVLVHNAARMGNDTAVETPSVALREALEVNVV
                     APNTLNYFTIPYMKPGSAIIYVGSTLAEKAVPGTYTYVVSKHALIGMMRSTCQDLAGQ
                     SIHTACVCPGFTDTEMLRQHVPAEAMASIRAMSAYGRLIDPDEIAETIFWAASNPVIN
                     GAVLHANLGQRES"
     gene            5807..6613
                     /locus_tag="AC16PP_01465"
     CDS             5807..6613
                     /locus_tag="AC16PP_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014631.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160254 - tRNA queuosine(34) biosynthetic
                     process from salvaged queuosine or its precursors
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HIR6480319.1"
                     /translation="MQGGVRTAGGNEETSGNRLSEQDLNERRERVFIVLAGFFLCAMT
                     LLNVVGITRFIQLGPMALAVGVLPYPLTFLCTDLISELYGRKRANFLVWVGFTLNFFI
                     LGMLWLGSSIPAVDPATQPPWQVIELAEEIALPNGDVLSGSVELFQLLYACTSGAVFA
                     SMMAYIAAQFCDVQLFHFFKRLTKGRHLWLRNNFSTLTSQMVDSFMVISVTFGAAYFA
                     GSITLAAMLVLMGSNYLFKMTVALADTLPFYYLTGRLKRYLQIDNVHVLE"
     gene            complement(6656..7591)
                     /locus_tag="AC16PP_01470"
     CDS             complement(6656..7591)
                     /locus_tag="AC16PP_01470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6480320.1"
                     /translation="MQMRLGITPWNLADRTASGLTEQARHAERLGYDSFWLPENHFNP
                     GAIPDPLMLLAAVAAGTQKIRLATTSYLLPLRNPLLAAEQVAVLDQLSGGRVTLGIGR
                     GYAPVMLRAFDVRPSEKRELFERSLALMRQAWRGEPVVLDDGETTVTLDPLPVQRPHP
                     PLWVAAFGPKALAQAGRLGLPYLASPVETLDTLEANYRRHGEAAAEAGHGPRDTIPVM
                     RTVFVSSNPRETRELLRRMNAEAEKSGRLAPGAEAHEWTIVGDDQFVRDRIVEYRERL
                     GVNHLIVTRLRLGGVDEGAVQASVGRVADIVGGLP"
BASE COUNT         1413 a         2567 c         2423 g         1323 t
ORIGIN      
        1 ggatagacgt tcgcaatgta ggtgccatga ccggaccttc cgcaaataaa ctgcccaagt
       61 ggcgacggtg taaccgtaac atatcttgac gtatttgaaa ctaggtcact tcccttggtt
      121 gcaggggctc aatacccctc ttccagcagc gggaacgcgc tgaaaaccgt ggactccgcc
      181 agaggctgag cccgctccat gaggcccgga ttgagatcgg caagatcgga gacgcccaga
      241 agcgccatgg tggtccggat ttcctgctcc acgatctcca gcgcccgaac gagggcccca
      301 cgtcctccgg ccgccatggc gaggccctcg agacgaccca tcccgaccac gtcggccccc
      361 aggcacaggc ccttgaccac atcggcacca cgcagaaaac cgccgtcgac gattaccggc
      421 acgcggccgg ccacggcctc gacgacctcc ggcagcgcgt cgatgcaggc cctggtgtgg
      481 tccagctgac gaccaccgtg gttcgaaacg taaacgacgt ccacacccgc ctccacgcag
      541 cgcgccgcgt cctcgcccgc gttgacgccc ttgacgatga gcggtatgtc gaacgtgctc
      601 ttgatgtgcg ccaccgtctc ccaggtcatt cgcgcctggt agttgaagtc ccggctgccg
      661 gcctggctgc ccgacggggg cacgaagccc ttgagaatgt cccgctcgcg cctggagtag
      721 atctgggtgt cggccgtcag gcagaagccc gtgtacccgg cttcgatggt ccggccgatc
      781 atgtcgtcca gccagacccg atcgcccatc aggtagagct ggtagatcct gggtccaggc
      841 accgcggcgg ccacggcctc gaaatcgggc gtgcacacgg agctcaggat ctggaggatg
      901 ccgaactcgt gcgccgcctc ggcaaccgtc tggccgcccc ccggctcgaa cagctgcacc
      961 gaaccgatgg gcggaagaac caccgggatg cgcatcggcg cgcccagcag ctcgatggtc
     1021 agattcacct cgctgacgtc gttcaggata cgcggacgaa agacccagga gtcgagccca
     1081 tggcggttgc gccgcagcga agactcggtg tccgcgccgc cgaggaggta gtcccggcag
     1141 tcgcgatcca gattgcgctg ggcctgcctc acgatctcat gcagcgtccg atacctcatc
     1201 gcgatttcca ccttcgatgc tcgaccgcgg atgctaacgc ctgcgccgga caaatccatc
     1261 gacaatcccg gtttcggctc gtcctgagca agggaacctt agtaagatgc ccctccagcg
     1321 cgggccggcg cacgggccgg ggcgagcgga cgaaccaagg agctgagcaa atgacgggga
     1381 tggttatcgt gggggccgga catgctgcgg ggcaggcggc tgccagcctg cgccaggagg
     1441 gttacgaagg tgagatcacc atcatcgggg acgagccgca cataccctac cagcggccac
     1501 ccctgtccaa gcagtacctg gccggagagc agggcctgga aagggtgtac ctgcgccccg
     1561 aaaagttcta cgcggacaag gaaattaccc tgcgcatggg cgtcagcgtc accgcgatag
     1621 acacccaggc acacaccgtg accacggacg acggcgaaac catcggctac gagaagctcc
     1681 tgctcgccac gggcagtcgg cccaggattc tcaacgtacc cggcagcgac cttgcgggca
     1741 ttcactacct gcggaccatt gccgacgtgg acggcattcg cgcggagctg gctgaagggc
     1801 gccggctcgt catcgtcggg ggcggctata tcgggctcga ggtggcgtca gtgggaatcc
     1861 aggcgggcct ggaggtccac gtgctggaga tggaagagcg catcctgcag agggtcacaa
     1921 cgcccgagat gtctgactat taccaccagc tgcacaccgg ccggggggtg cacatccata
     1981 cctccaccat ggtgagcggc ttcaccggcg agggccgcgt cggcgcagtg cagtgcggcg
     2041 acgaggagat tgccgccgac atcgtggtcg tgggcatcgg catcatcccc aacgtcgagg
     2101 ttgcccaggc cgccgggatc gagtgcgaca acggcatcgt cgtggacgaa ggctgccgca
     2161 cgtcggcgga cgacgtgttc gccgccggcg actgcaccaa tcatcccaac ccgctgctcc
     2221 accggcgcct gcgcctggaa tccgtgccca atgccatgga gcaggcccgg gtagcggccg
     2281 cgaacatgtg cggcgcagac aaggtgtacg agtcgattcc atggttctgg tcggaccagt
     2341 acgagctgaa actccagatg gtcggcttct ccgccgacgg cgacacgcag gtggtccggg
     2401 gcgacaaggc agccaaccag tttgccgtgt tctacctcaa agacggcgcg gtggtggccg
     2461 ccgacgcggt gaacagcccg aaggagttca tggtgtgcaa gcagctcgtg ggcaagccgg
     2521 tggatgcggc caggctgggc gatccggatg tggagctgaa gtccctgctg gcttgagcag
     2581 cctcaaaggc ggccgatcga cacctttgcc gtccgtgcca tacgtagaat ccctgatggg
     2641 tatgggcaca gggaaagatg acaatgattt ctgcgacagg tgcgcgggat cgtctcctcc
     2701 tctccccttc ccgcgcgtct tttcacgccg gatcctctgg atccggcgtt tctttatggg
     2761 cccacttcgc ccggggccgc tcagtccacg gacagggaaa ggacccggga aatctggcac
     2821 agctcgcgcc cgctctccgc gtgccattcg ttgaaggcgt cctggacccg cgcgagcgct
     2881 ttcttgctgg tgggctcccg atcgatcacg ccctgaccta tcagggcttt gacgacgtct
     2941 ctggacagga tcggcgtgtc tttgccgacg aaacgcagaa agtaacgacc ggtctggccc
     3001 ccgagccgcg agccgcgacg cttgagctcg tcccacaaac cgacgatctc gtcttccggc
     3061 cagtcggcca ggtagcgccc gaagccaccg gcttcacggc gcacgtccag aatgaaggcc
     3121 gcgttggcag gaaccgaacg gatcttcgct gcgtgacgca cgatccgctc gtccgccgag
     3181 agctgctcga tgtcttcatc ggacagcatc gcacagccgg ccacatcgaa cccgcggaat
     3241 gcaacctcga accccggcca cttgttctcg atgattttcc acacgaaacc cgagcggaag
     3301 acacacctgg tcatctcggc gaggtagtcc cggtccggca aggcggccag atcgctgcct
     3361 gacctggctg cgggcagcag cgcgtctagg gccgccctgc cccccttgcg ctcaaccgcc
     3421 ctgtgcaaga tgctatcgaa cgattccata tcccgcggaa cccaaagagc cacgacgaac
     3481 accatggtgg atgtcctcga caaaacccgt caagacctag acccgaagct caaggagatc
     3541 gtcgagaagg acgagcggaa tttcgtctac tgcgggatct gctcgcacgt gctgacccgg
     3601 gaggtcgagc gcatcgaggt gtcgggcagt cacgatcatc attgcacgaa cccgttcggt
     3661 cttcgctttc atatcggctg cttcgccgag gccctgggct gcgacatctc gggagagccc
     3721 atggcggcag acacctggtt tccgggcttc cgctggcgcc acgcatcctg cgcggagtgt
     3781 caccagcatc tgggctggta tttcgaagca ggcgaaagat acttctacgg attgatactg
     3841 gaccgcattc agcatgactg acctcgctgc cggcaactac cgcacgctga ccttcgactg
     3901 ctacgggacg ctcatcgatt gggagaacgg catcctcggc tacctgcagc ccctgctcga
     3961 gagctacgac gtgcacgtgc tcgacgagtg ggtgctggaa ttctttgcgg agaacgaacc
     4021 cgcgctgcag gccgagggcg gctcgtatcg atcggtgctc gcgcgtctgc tggagcggct
     4081 cggtacgcgg tgcgcgttca cgcccaacga tgacgccctg aagggctttg cagacagcat
     4141 cgagtactgg cagccgttcg cagacaccaa cgccgcgttg cgcgccctgt ccgagcactt
     4201 cgacctggca gtggtctcga atgtcgacaa cgagctgttc tccctctccg ccaggcagct
     4261 gggcgtggag ttcgcgcagg tgatcactgc cgatgacgtc ggggcctaca aacccgcccg
     4321 cgcgatgttc gacgaggcgc tcagaaaggt atcggggccc gtgcttcacg tcgcccagag
     4381 cctctaccac gacatcgcgc ccgccaaagc gctgggtctg gacaccgtgt ggatcaaccg
     4441 gccgagcgcg gccggcagcg gggccgcgcg tttcgtcgag gtggaaccga cctggacgtt
     4501 cggcgatctc agcgagctcg tcgaggcctt cgacctcgac tgagccgatg cggaattcac
     4561 ccgttcgcac gttttcgctg tgataggatc gcccgatgtc gcgaccgtct ctcaccagca
     4621 ttcagatcag caaggaatac cttcatttca actccgctca cttcaccctg ttctccgatc
     4681 gcgaacggga ggacctgcac ggccacacct tttacgtggc gggtgctttc gatgccgagg
     4741 tgggtccgga cgggctggcg ttcgattaca acctggtcaa gaccaagctg aaacagctgt
     4801 gcgacgagct ggacgagaaa acccttctgc ccgaacgttc tccccacctg cgcctgagcg
     4861 aggaaaacgg ctatctcgtc gccgagttcg ccggcgagcg catgcccttt ctgccacggg
     4921 acgtcctcac ccttcccgtg cgcaacgtca cggtagagga actggcgccg tttctgctgg
     4981 agcgtctcgg ctcggatccc gagatccgca cgctcgagct gcagaggatt gagctgcgcg
     5041 tctcttcagg acccggccag tgggctgcgg cgacctggac cgccgggcaa gagatacggg
     5101 aggtgtaagc catgaagata ctgctcatta ccggagccag cgcggggatc gggctgcaca
     5161 ctgcccaacg cttcgttgcc gaaggattcg ccgtggtgaa cctctcgcgg cgccgctgtc
     5221 ccatcgacgc ggtcagccag atcaactgcg acctgtcggt tcccgggttc atcgacaaca
     5281 tcagcaatca gctcagcgga atgctgcagg aggccgaccg ggtggtgctc gtgcacaacg
     5341 ccgcccggat gggcaacgac acggcggtgg agaccccgag cgtggccctg cgcgaggcgc
     5401 tggaggtgaa cgtggtcgca ccgaacacgc tcaactactt caccatcccg tacatgaaac
     5461 cgggctcggc catcatctac gtgggctcca ccctggcgga gaaggcggta cccggcacgt
     5521 atacctatgt ggtgagcaag cacgccctga tcggaatgat gcgctcgacc tgccaggatc
     5581 tggcggggca gagcatccat accgcgtgcg tttgcccggg cttcacggat accgagatgc
     5641 tgcgtcagca cgttccggcc gaagccatgg cgagcatccg cgccatgtcg gcctacggac
     5701 ggctgatcga cccggacgag atcgccgaaa ccatcttctg ggccgccagc aacccggtta
     5761 tcaacggggc cgttctgcac gccaatctgg gtcagcggga aagctgatgc agggaggcgt
     5821 aagaacagcg gggggcaacg aggaaacatc gggaaaccgc ctcagcgagc aggacctgaa
     5881 cgagcgacgc gagcgggtgt tcatcgttct ggccggcttt ttcctctgcg ccatgacgct
     5941 gctaaacgtc gtcggcatca cccgtttcat acagctgggc cccatggccc tggcggtggg
     6001 cgtgctgccc tacccgctca ccttcctgtg cacggatctc atcagcgagc tgtacggccg
     6061 caaacgcgcc aacttcctgg tctgggtggg cttcaccctg aacttcttca ttctcggcat
     6121 gctctggctc ggcagctcga taccggccgt agatcccgcc acgcagccgc cctggcaggt
     6181 catcgagctg gccgaggaga ttgcgctgcc caacggcgac gtcctcagcg gcagcgtgga
     6241 gctgtttcag ctgctctatg cctgcacctc gggcgccgtg ttcgcttcga tgatggccta
     6301 catcgccgcg cagttctgcg acgtgcagct ttttcacttc ttcaagcggc tgaccaaggg
     6361 tcgacacctg tggcttcgca acaacttttc cacgctgacg agccagatgg tggactcctt
     6421 catggtcatc agcgtcactt tcggcgcagc ctacttcgcc ggcagcatca cgctggcggc
     6481 catgctcgtt ctgatgggca gcaactacct cttcaagatg acggtggcgc tggcggacac
     6541 cctgcccttc tactacctca cgggtcgcct caagcgctac ctgcagatcg acaacgttca
     6601 cgtcctggag tgaccggccc gatcggatcg ctccggcgtg atccggtgga ggcagtcagg
     6661 gcagaccgcc gacgatgtcc gccacccgcc cgacggaagc ctgcacggca ccttcgtcca
     6721 ccccgccgag cctgagtcgc gtcacgatca ggtgattgac ccccagccgc tcgcggtact
     6781 cgacgatccg gtccctgacg aattggtcgt ctccgacgat ggtccactcg tgagcttccg
     6841 cgccgggcgc caggcgaccg gatttttccg cttccgcatt catccggcgg agcagctccc
     6901 gtgtctctct cgggttggac gaaacgaata ccgtgcgcat caccgggatc gtatcgcgag
     6961 gcccgtggcc ggcctccgct gccgcctcgc cgtgacgccg ataattggct tcgagcgtat
     7021 cgagggtctc cacgggcgac gcgaggtaag gcagcccgag acggcccgcc tgggcgagtg
     7081 ccttcggccc gaaggcggcg acccacagcg gcgggtgcgg gcgctgaacc ggcagcggat
     7141 ccagagtgac ggtggtctcg ccgtcatcca gcacgaccgg ctcaccgcgc cacgcctgcc
     7201 gcatgagggc taaggagcgc tcgaagagct cgcgcttctc gctgggccgg acgtcgaacg
     7261 ctcgaagcat gaccggcgcg tacccccgac cgatcccgag cgtgacccga ccgccggaga
     7321 gctgatcgag cacggcaacc tgctcggcag ccagcagcgg atttcgcagc ggcagcaggt
     7381 acgacgtggt ggcaagacgg atcttctgcg tgccggccgc caccgcggcc agcagcatga
     7441 gcggatccgg tatggcgccg gggttgaagt ggttttccgg aagccagaac gaatcgtacc
     7501 cgagccgctc ggcatgccgg gcctgctcgg tcagcccgct ggcggtgcga tcagcgagat
     7561 tccagggcgt tatgccaagc cgcatctgca tgccacctca ccacgccgtc acgacagcac
     7621 gtccgcagat gccagctcgc ggacccggtc gccgtcgtag ccgagaatct cgcttagcac
     7681 gcgctcgttg tcgatgccga accacggcgc cgtgccgggt cgggcg
//
LOCUS       DBNHKV010000050         7527 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_858023, whole genome shotgun sequence.
ACCESSION   DBNHKV010000050 DBNHKV010000000
VERSION     DBNHKV010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7527)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7527
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_858023"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /gene="queA"
                     /locus_tag="AC16PP_01475"
     CDS             <1..791
                     /gene="queA"
                     /locus_tag="AC16PP_01475"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR6480321.1"
                     /translation="LVERIEGETEALCQVRVSKALKPGRSIQIGDAELEVLGRKGDFY
                     SLRFPSDVLAFLEQRGETPLPPYIERAAGTVDADRYQTVYGRRPGAVAAPTAGLHFTE
                     DLLRQVRDRGVEIAEVTLHVGAGTFQPVRVDDISQHRMHAERYEICSRAARALAECRE
                     RGGRIVAVGTTVVRTLESAAARSGEVEAGSGETDLFITPGFPFRVVDVLLTNFHLPRS
                     TLLMLVCAFAGYERVMAAYAEAVDEGYRFFSYGDACFMERTDEL"
     gene            781..1896
                     /gene="tgt"
                     /locus_tag="AC16PP_01480"
     CDS             781..1896
                     /gene="tgt"
                     /locus_tag="AC16PP_01480"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR6480322.1"
                     /translation="MSFELLGTDGAARRGRLKTAHGTVETPVFMPCGTYGTVKAMTPR
                     ALKEVGTQMLLGNTFHLMLRPGERLIESLGGIHEFMHWDGPVLTDSGGFQVWSLGELR
                     KISEEGVVFRSPVNGDRVELTPERSVAVQRALGSDVLMVFDECTPYPATEAEARLSME
                     LSMRWAERSLRAHQPSSGGLLFGIVQGGMFPALRQASLELLVELDFPGYAIGGLSVGE
                     SKSEMRKVLDRLLPLMPTTKPRYLMGVGTPEDLVRAVGLGVDMFDCVMPTRNARNGYA
                     FTSAGTLKIRNARHRESDLPLDERCGCYTCANFSRAYLHHLDKCGEMLGSMLMTHHNL
                     HYYHWLMAELRASIESGKFRTLIETLRTDWNSPDEPI"
     gene            1883..2224
                     /gene="yajC"
                     /locus_tag="AC16PP_01485"
     CDS             1883..2224
                     /gene="yajC"
                     /locus_tag="AC16PP_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR6480323.1"
                     /translation="MNLFETTAYAADAGGPPPDAGMINLLFLGGFILIFYFLLWRPQS
                     KRRKEHQALMSGLAKGDEVVTTGGIVGQVTRVDDDFVKLQVANNIELRVQKSSVGATL
                     PKGTLKSLDGD"
     gene            2300..4174
                     /gene="secD"
                     /locus_tag="AC16PP_01490"
     CDS             2300..4174
                     /gene="secD"
                     /locus_tag="AC16PP_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR6480324.1"
                     /translation="MSKARRRAPNTHSVGRYLFILFVVVIGGVYAAPNLFQPDPALQI
                     KSTNEANPLNQEVLERVTAALAARGVTVKGTELAGDSALVRVGTDEQQLRGRDIATAE
                     LNPESSGRYVVALNRASTTPQWLQDAGGKPMSLGLDLSGGVHFLLQVDMEKFLGDRMR
                     SNQEAIRDLLVDARIRYVGRDWLDGRQLSIPFQTAEGRDEASGLIAETFDGFEIQERD
                     VDGRPGLRLVLDDAKIGELEDLAISQNLQSLRNRVNELGVSEPLVQRLGRSRIVLDLP
                     GIQDSARAKEIINKFANLEFRLVAAPDARSSQTENYDYEGAPVSLERRNIVTGDQVTN
                     AVQEYDPESGMPQVSITLDNDGGRAMNEVTKDHVGDSMAILFKELKVRTRRVVEDGEE
                     KLQPYTVEEKRLINVATIQSALGFRFRITGLGLGEARDLALLLRAGALAAPMYIVEER
                     TVGASLGEENIEKGRQSIVIGFLLVMVFMLVYYRWFGLAANVALTMNLVLIVAIMSVL
                     GATLTLPGIAGIVLTVGMAVDANVLIFSRIREELTDRPPQQAISAGFDRALLTILDAN
                     ITTFFVAIILFSVGSGPVKGFAVTLAVGVVTSMFTAIMGTRALVNLMYGGRSLERLSI
                     "
     gene            4171..5097
                     /gene="secF"
                     /locus_tag="AC16PP_01495"
     CDS             4171..5097
                     /gene="secF"
                     /locus_tag="AC16PP_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019823695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR6480325.1"
                     /translation="MKPLNLNIDFMGKRRIAGGISMVLVLVSLIAVFSVGLNLGLDFT
                     GGTLVEVEFVEPTNPNTVRELLENDGYSNGVVQYFGTERDLLIRMPPQEDGEQANMGD
                     EILAVLSARYPGVVMRQSNYVGPAVGEELTEDGGLAVLAALFVVMLYILFRFTKQFSV
                     GAVVALIHDVAIVLGIFAMAQWTFDLPVLAALLAVIGYSLNDTIVVSDRIRENFRKIR
                     KGAAVDIINTSLNQTLGRTLVTSLTTLLVLLALLFAGGEMIRGFALALTIGVLVGTYS
                     SIYVAANVLLVMNITREDLLIPEKENPDQAVP"
     gene            complement(5098..5898)
                     /locus_tag="AC16PP_01500"
     CDS             complement(5098..5898)
                     /locus_tag="AC16PP_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR6480326.1"
                     /translation="MQAMANMALRAARQAGQIMTRAMDRVDLLQVEEKARNDLVSNID
                     REAEQAIREVLLKAYPEHGILGEEYGETNPEAEYVWVVDPLDGTTNYLQGIPHFCISI
                     ACMRGRQIEHAVIVDPVLNEEFVASRGSGAQLNGKRIRVSRTEKLRDAVVGTGIPPKA
                     IARHLEPYMAMLHDFVGTCRAIRRSGSAALDLAYVAAGRLDGFWELALSPWDIAAGTL
                     LVREAGGFVGDLSGGDAFMQHGNIVAANPKCFKAMVTRMRPNLTPELA"
     gene            6005..6742
                     /locus_tag="AC16PP_01505"
     CDS             6005..6742
                     /locus_tag="AC16PP_01505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005760054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HIR6480327.1"
                     /translation="MSEVLKGIRIVLVETSHPGNIGGAARAMKTMGLSDLVLVNPKRY
                     PDPQAQWRAAGAQDVLEAARVCADLTEAIEGCHWVVGTSTRSRRIPWPVLSPEEVARQ
                     AVERAGSSIAILFGREANGLSNEELQRCHCHLQIPAAPDYPSLNVAMAVQVVCYELRK
                     LAGEAGDEIGAWDRPTATVAQLDGLIEHLEEVMVASGFLDAANPGQTMTRLRRLFSRM
                     EPDTTEVQILRGILTHLHPLRRPGPGE"
     gene            6801..7259
                     /locus_tag="AC16PP_01510"
     CDS             6801..7259
                     /locus_tag="AC16PP_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HIR6480328.1"
                     /translation="MRLTTKGRYAVTAMLDIALHRDAGPVSVVDIAHRQDISAAYLEQ
                     LFSKLKRAGLISSIRGPGGGYQLALDAEAINVSRIIAAVGEGVDATRCHGAGDCQDGL
                     TCLTHDLWTELSQEIDSFLGGITLASLVRKRGVRSIASRQDSQLIEARLI"
     gene            7259..>7527
                     /locus_tag="AC16PP_01515"
     CDS             7259..>7527
                     /locus_tag="AC16PP_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR6480329.1"
                     /translation="MRKAVYLDYAATTPVDPRVAQKMSACLLVEGNFGNPASRSHVYG
                     WQAEEAVEHARTQVAKLINADPREIVWTSGATESDNLAIKGVADGA"
BASE COUNT         1297 a         2338 c         2476 g         1416 t
ORIGIN      
        1 tgctggtcga gcgaatcgag ggcgaaaccg aggcgctgtg tcaggtccgg gtcagcaagg
       61 cgctcaaacc aggccggtcc atccagattg gcgatgccga actcgaggtg ctcggtcgaa
      121 aaggggattt ctacagcctg cgctttccgt cagacgtgct cgcattcctg gaacaacgcg
      181 gcgaaacgcc gctgccgccc tacattgagc gagcggccgg gaccgtggat gccgatcgct
      241 accagaccgt atacggacgc cggcccggcg cggtggcggc gcccaccgcc ggcctgcact
      301 tcacggaaga tctgctccgg caggttcgcg accgaggggt ggagatcgcc gaagtcactc
      361 tccacgtggg cgccgggacg ttccagcccg ttcgggtgga cgacatctcg cagcaccgga
      421 tgcatgccga gcggtacgag atctgcagcc gcgcggctcg tgctctggcc gagtgccggg
      481 agcgcggagg ccggatcgtc gccgtgggca ccacggtggt ccgcaccctc gaaagtgccg
      541 cggctcgaag cggcgaagtc gaggcgggct cgggcgagac cgatctgttc atcacgcccg
      601 gatttccgtt tcgcgtggtg gatgtgctgc tcacgaattt ccacctgccg cggtccaccc
      661 tgctgatgct cgtctgcgcg tttgcaggtt acgaacgggt gatggccgcc tatgcggagg
      721 cggtcgacga gggatatcgc tttttcagct acggagatgc ttgcttcatg gagcgaaccg
      781 atgagctttg agttgcttgg caccgacggc gctgcccggc gcgggcgcct gaaaacggcg
      841 cacggcacgg tcgagacacc ggttttcatg ccctgcggaa cctacgggac cgtcaaggcc
      901 atgacgccgc gcgcgctgaa ggaggtaggg acgcagatgc tgctcggcaa tacctttcat
      961 ctgatgctgc gccccggaga acggctcatt gaatccttgg gcggcattca cgagttcatg
     1021 cactgggatg gtccagtcct caccgacagc ggtggctttc aggtttggag cctgggagag
     1081 ctgcgcaaga tcagcgagga gggcgtggtt ttccgctcgc cggtgaacgg cgatcgtgtc
     1141 gagcttacgc ccgagcgctc ggttgccgtc cagcgcgctc tgggcagcga cgttttgatg
     1201 gtgttcgacg agtgcacgcc ttaccccgcg acggaagccg aggcccgcct gtccatggag
     1261 ctgtcgatgc gctgggcgga gcgcagcctg cgtgcgcacc aaccctcgtc cggtggtctc
     1321 ctcttcggga tcgtccaggg cggcatgttt ccggctctgc gtcaggcgag cttggagtta
     1381 ctggtcgagc tcgacttccc cggttacgcc atcggcgggc tctcggtggg ggagagcaag
     1441 tcggagatga ggaaggttct agaccggctg ttgccgctga tgcccacgac caaaccaaga
     1501 tatctcatgg gggtcggcac gcctgaggac ctcgttcgtg cagtggggct cggcgtggac
     1561 atgttcgatt gcgtgatgcc cacgcgcaac gcgcgcaacg gctacgcctt cacgtcggcg
     1621 ggaaccctga agatccgcaa cgccaggcat cgggaatcgg acctgcccct cgatgagcgc
     1681 tgcggctgct acacctgcgc gaatttctcg cgggcctatc ttcatcatct ggacaaatgc
     1741 ggcgagatgc tgggcagcat gctgatgacg caccacaacc tccactacta ccactggctg
     1801 atggctgaat tgcgcgcctc catcgaaagc ggtaaatttc gcaccctcat cgagaccctt
     1861 cgaaccgact ggaactcccc ggatgaacct atttgagacc actgcctacg cggccgatgc
     1921 cggcggtccg ccgcccgatg ccggcatgat caacctgctt ttcctcggcg gtttcatcct
     1981 catcttctac tttctcctgt ggcgccctca gagcaagcgc cgcaaggaac atcaggcgct
     2041 gatgtcgggc ctggccaaag gggatgaggt tgtcaccacg ggcggcatcg ttggtcaggt
     2101 gaccagggtg gatgacgatt tcgtcaagct ccaggtggca aacaacatcg agctgcgcgt
     2161 gcagaagagc tccgtcggcg cgacgctgcc taagggcacg ctgaaatccc tggacggcga
     2221 ttagtgcgcg gctggcaagc gcggagaacg tccagccgtg gctaattcga gtggtgtggt
     2281 gggacaaggc ctgctcgggt tgtcgaaggc gcggcgccga gcgcccaaca ctcactccgt
     2341 cgggcggtat ctgttcatcc tgttcgtcgt ggtcattggc ggcgtatacg cggcgcccaa
     2401 cctgtttcag cccgatcccg cgctgcagat caagagtacc aacgaagcca acccgctcaa
     2461 ccaggaggtg ctggagcggg tgacggcggc gctggcagcg cgcggcgtaa cggtaaaggg
     2521 caccgagctg gccggcgatt ccgctctggt ccgggtcggc acggacgagc agcagctgcg
     2581 cgggcgcgac atcgccaccg ccgagctcaa cccggagtcg tcgggccgct acgtggttgc
     2641 cctgaaccgc gcctcgacca ccccgcagtg gcttcaggat gccggcggca agcccatgtc
     2701 gctaggcctc gatctctcgg gcggcgtgca tttcctgctt caggtcgaca tggagaagtt
     2761 cctcggcgac cggatgcgca gcaaccagga agccatccgc gatctgctgg tagatgcccg
     2821 catccgctac gtgggacgag actggctcga cggcaggcag ctgagcattc ctttccagac
     2881 cgcagagggc agggacgagg ccagcgggct gatcgccgag acattcgacg gctttgagat
     2941 ccaggagcgc gacgtggatg gccggccggg cctgcggctc gtgctcgacg atgcgaaaat
     3001 cggggagctg gaagacctcg ccatatccca gaacctgcag agcctgcgca accgggtcaa
     3061 cgagctgggg gtttccgagc ctctggttca gcgcctcggg cggtcccgta tcgtgctcga
     3121 cctgcccggt atccaggata gcgcccgggc gaaggagatc atcaacaagt tcgccaatct
     3181 ggagttccgt ctcgtcgccg cgcccgacgc ccgttcctcc cagaccgaaa attacgatta
     3241 cgaaggggcg cccgtgtccc tggagcgacg caacatcgtc actggtgatc aggtgaccaa
     3301 cgcggttcag gaatacgacc ccgagtccgg catgcctcag gtgagcatca ccctcgacaa
     3361 cgacggcggc cgggcgatga acgaggtgac caaggatcat gtcggggact ccatggccat
     3421 tctgttcaag gagctcaagg tccgcacccg tcgtgtggtc gaggatggcg aggaaaagct
     3481 gcaaccctac accgtggagg agaaacgcct gatcaacgtg gccaccatcc agagcgcgct
     3541 gggattccgc ttccgcatca cggggctggg tctgggcgag gcccgcgatc tcgctctgct
     3601 gctccgagcc ggcgccctgg ccgctcccat gtacatcgtc gaggagcgga ccgtcggggc
     3661 gagcctcggc gaggagaaca tcgagaaggg gcgtcagtcc atcgtcatcg gcttcctgct
     3721 ggtcatggtg ttcatgctgg tctactaccg atggttcggg ctggcggcta acgttgccct
     3781 gaccatgaac ctggttctga tcgttgccat catgtcggtg ctcggcgcca ctctgacgct
     3841 cccaggcatc gcggggatcg tgctcacggt cggcatggcg gtggacgcca acgtgctgat
     3901 cttttcccgc atccgcgaag agctcacgga ccgtccccct cagcaggcga tatctgccgg
     3961 cttcgacagg gctctgctga cgattctcga cgcgaacatc acgaccttct tcgtggccat
     4021 cattctcttt tccgtcggca gcggtcccgt caaagggttc gctgtcacgc tggcggtcgg
     4081 ggtcgtgacg tcgatgttca cggccatcat ggggacgcga gcgctggtca acctgatgta
     4141 cggcgggcgc tcgctcgaga ggctctccat atgaagccct tgaacctgaa catcgatttc
     4201 atgggcaagc gccgcattgc cggtggtatc tccatggtcc tggtgctggt ttctctgatc
     4261 gccgttttct cggtcggcct gaacctcggc ctcgacttca ccggcggaac tctggtcgag
     4321 gtggagttcg tggagccgac taacccgaac accgtgcgcg agctgctgga gaacgacggc
     4381 tacagcaacg gcgtcgtcca gtacttcggg accgagcggg atctgctcat ccgcatgccg
     4441 ccccaggaag acggcgagca ggccaacatg ggcgacgaga tccttgcggt tctcagtgcc
     4501 cgctatcccg gcgtggtcat gcgccagtcg aactacgtgg gtccggcagt gggtgaggag
     4561 ctaacggagg acggcgggct tgcggtgctc gccgcgctgt ttgtcgtgat gctctacatc
     4621 ctgttccggt tcaccaagca gttttccgtc ggtgccgtgg tagcgctcat tcacgacgtt
     4681 gccatcgttc tcggaatatt cgccatggcg cagtggacgt tcgaccttcc ggtccttgcg
     4741 gcccttctgg ccgtcatcgg ctactcgctg aacgatacca tcgtcgtctc ggaccgcatt
     4801 cgcgagaatt tccggaagat ccgtaaaggc gcggccgtgg acatcatcaa tacgtcgctc
     4861 aaccagaccc tgggacgaac gttggtcacg tcgttgacca cgctgctggt gctgctcgcc
     4921 ctgctgttcg ctggcggaga gatgatccgc gggtttgccc ttgccctgac catcggcgtt
     4981 ctggtgggga cctactcgtc gatttacgtg gcggccaacg tgcttctggt gatgaacatc
     5041 acccgcgagg acctgctgat accggagaag gagaatccgg accaggcggt gccctgatca
     5101 ggcgagctcg ggggtcaggt tcgggcgcat gcgggtaacc atggccttga agcatttcgg
     5161 gttggccgcg acgatgttgc cgtgctgcat gaaggcatcg cccccagaga gatcgccgac
     5221 gaaacctccc gcttcgcgca ccagcaacgt gccggcggcg atgtcccagg ggctcagcgc
     5281 caactcccag aatccgtcga gccgcccggc cgcgacgtag gccaggtcga gcgccgcgga
     5341 gccgctgcgt cgtatggcgc gacaggtgcc gacgaaatcg tgcagcatcg ccatgtaggg
     5401 ctcgaggtgc cgcgcgatgg ccttcggcgg gatgccggtc ccgacaaccg cgtcgcgaag
     5461 tttctcggtt ctgctgacgc gtatgcgttt cccgttcagc tgtgcgccgg acccgcggct
     5521 ggcgacgaac tcctcgttaa gtaccgggtc gacgatcacc gcatgctcta tctgacgtcc
     5581 tcgcatgcac gcgatggaaa tgcagaagtg cgggattccc tgaaggtagt tggtggtgcc
     5641 gtccagcggg tcgaccaccc agacgtactc ggcctcgggg ttggtctcgc cgtattcttc
     5701 gcccaggatg ccgtgctcgg ggtaggcctt caggagcacc tcccgaatgg cctgctcggc
     5761 ttcccggtcg atgttggata ccagatcgtt gcgtgctttc tcctccacct gcaggaggtc
     5821 gacgcgatcc attgcccggg tcatgatctg cccggcctgg cgcgctgcgc gcagagccat
     5881 gttcgccatt gcctgcatgg tgttgaaagc ggtaccaaag aggagggcgg ctatcctaac
     5941 aacgtgcttg caggtaggga atgtcggaaa atggccgatt gctgttgagc actggaagcc
     6001 gattgtgtct gaggtattga agggaatccg catcgtcctc gtggagacga gtcatccggg
     6061 caacatcggc ggcgctgcgc gggcgatgaa gaccatgggt ctttccgacc tcgttctcgt
     6121 caacccgaag cgctaccctg atccgcaggc gcagtggcgc gcggcaggtg cccaggacgt
     6181 gctggaggcc gctcgtgtct gcgccgatct caccgaggct atcgaggggt gccactgggt
     6241 ggttggcacg agcacccgca gccggcgcat tccctggccc gtgctgtccc cggaggaggt
     6301 cgccaggcag gcggtcgagc gggcgggcag ctccattgcc atcctgttcg gccgggaggc
     6361 caatgggctc agcaacgagg agctgcagcg atgtcactgt cacctgcaga ttcccgcggc
     6421 ccccgattac ccctcgctca acgtcgccat ggcggtgcag gtggtgtgct acgagctgcg
     6481 caagctggcc ggcgaggccg gagacgaaat cggcgcctgg gatcgcccca ccgcgacggt
     6541 cgctcagctc gacgggctga tcgagcatct cgaagaggtg atggtcgcca gcggctttct
     6601 ggacgccgcg aatccgggac agaccatgac ccgcctgcgc cggcttttct ccaggatgga
     6661 accggatacg acggaggtcc agatactgcg cgggatcctt acccacctgc atcccttgcg
     6721 gcgtcccggc cccggggaat agtggaccat tttagtcggg tattgcatac tgcctggctt
     6781 gtatcgatgg tgggccatcc atgcggctga cgacaaaagg cagatatgcg gtcacggcta
     6841 tgctggacat tgcgctgcat cgcgatgccg gcccggtgag cgtcgtggac atcgctcatc
     6901 gtcaggatat ctccgccgcc tatctcgagc agctgttcag caagcttaag cgcgccgggc
     6961 tcatctccag cattcgcggt ccgggcggcg ggtatcagct cgctctcgac gccgaagcga
     7021 tcaacgtgtc ccggatcatc gcggcggtgg gggaaggcgt ggacgccaca cgctgtcacg
     7081 gcgcggggga ctgtcaggat gggttgacct gcctgacgca cgatctttgg acggagcttt
     7141 ctcaggagat cgactcgttt ctcggcggca tcaccctggc cagcctggtg cggaaacgag
     7201 gtgtgcgcag catcgcttcg cgtcaggaca gccagctgat cgaagcgcgg ctgatctgat
     7261 gcgcaaggcc gtctatctgg attacgccgc gaccaccccg gtggatccgc gggtcgccca
     7321 gaaaatgagt gcctgcctgc tggtggaggg aaatttcggc aatccggcgt ctcgctccca
     7381 cgtctacggc tggcaggccg aggaggcggt ggagcatgcc aggacccagg tcgcaaagct
     7441 catcaacgcc gatccgcggg aaatcgtgtg gacctccggc gcgacggagt ccgacaacct
     7501 ggcaatcaaa ggagttgccg acggcgc
//
LOCUS       DBNHKV010000051         3755 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1778584, whole genome shotgun sequence.
ACCESSION   DBNHKV010000051 DBNHKV010000000
VERSION     DBNHKV010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1778584"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="AC16PP_01520"
     CDS             <1..672
                     /locus_tag="AC16PP_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480330.1"
                     /translation="QRFGAQGGDWGALVTAQLGHKYAESLFGIHLTNLIPLTLFNHQR
                     PWDITAGVMVPDDVPADERDRQLAQQRRIASHVAVQVLDPQTLAYGLHDSPVGLLAWL
                     LERRYFWGDCNGDVESRFSREHLITTAMIYWVTSSFATSARYYAEAARNPWRPSHDRS
                     PQVEAPAGATYLGGDVGRGTIPAVREAFDLRYENSHPVGGHFAPMEEPEVVINDIRET
                     FRPLR"
     gene            complement(700..1221)
                     /locus_tag="AC16PP_01525"
     CDS             complement(700..1221)
                     /locus_tag="AC16PP_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6480331.1"
                     /translation="MTLPISATFHHVGITVSNLERAVEWYSKVFGLKPGVALEVSGPT
                     GEQVLRLPPHIHRAALLPVGDFAIELLEFEPTRRQMDMRQDDVGYVYICFAVDDLDDV
                     YARLSAEGIDFHTPPMLAEEGQIAGSKFCVMRDPDGKTIELVEPGPGMKVPALHAAAR
                     RGVALDDPLIMDG"
     gene            complement(1218..1781)
                     /locus_tag="AC16PP_01530"
     CDS             complement(1218..1781)
                     /locus_tag="AC16PP_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480332.1"
                     /translation="MRIEPLKGWHRYTELMNAETDPRRKAMLDNMRHHLKYECLGDPA
                     IFDSMVPDPEYRFYASFDNAVLTGMEEVREFYYNIWNTRSSLVELAIHRCAAADWGVA
                     CEGEWYQQVPGETLIANGQQVEDRDAWYLSHAHLSWFFPFREIDGRMLLEGEICYIDE
                     PGSTLQKLDPADVITLEEAKASWAALP"
     gene            complement(1874..2545)
                     /locus_tag="AC16PP_01535"
     CDS             complement(1874..2545)
                     /locus_tag="AC16PP_01535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR6480333.1"
                     /translation="MLVAAIAATFAGSAALAGLVFWPAGTFAWPRAWGYLALVGANFV
                     VNLVYLQRVNPSLIAARARFGAGTKRWDVIWSVCFAPAFCSIYVVAGLDAVRYEWTAM
                     SDRLWWLGLAVFVPGTSLFSWSMGVNPFFEKTVRIQTERGHRVVDAGPYQIIRHPGYV
                     GFFGWCFSAPLLLGSWWAFVPATISALGMVVRTALEDRTLQRELPGYADYARRVRYRL
                     LPGVW"
     gene            2833..>3755
                     /locus_tag="AC16PP_01540"
     CDS             2833..>3755
                     /locus_tag="AC16PP_01540"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6480334.1"
                     /translation="MSRKRTGHRGSQPYSSDPQTERSLRHSIKDGVYFSAMTGGAESY
                     FSAFAIHLNASTTIIGLLASLPPLLASFMQMLSAWLGRRTGRRKPIIVAGALLQAALL
                     VPIAAVPLLWPDAAAVLLIPLVFVYLCGPNLGAPQWNSLIGDLLPEARRGRFFALRTR
                     LSSVASLAALVIAGLVLEAFDKSGDAYWGFVVIFLLAGAARCVSAYHLNAMTEPTRNV
                     AGLLAPDDLSVWRRVRHSSLLRFSLFFAAMQFAVAISGPYFTLYMLRDLALSYVEFMV
                     LSFVSVTIQFLTLNRWGRLADLFGNRLILITT"
BASE COUNT          611 a         1239 c         1238 g          667 t
ORIGIN      
        1 cagcgcttcg gcgcccaggg cggcgactgg ggcgcgctgg tgactgccca gctgggccac
       61 aagtacgccg aaagcctctt cgggatccac ctgacgaacc tgatcccgct gacgctcttc
      121 aatcatcagc gtccgtggga catcaccgcg ggcgtgatgg ttcccgatga cgttcccgcg
      181 gacgagcgcg accggcagct cgctcagcag cggcgcatcg ccagccacgt ggcggtgcag
      241 gtgctcgatc cgcagacgct ggcgtacggg ctgcacgact cccccgtggg gctgctggcg
      301 tggctgctgg aacgccgcta cttctggggc gactgcaacg gcgacgtgga atcccgcttc
      361 agccgcgagc acctgatcac cacggcgatg atctactggg taacgtccag cttcgccacg
      421 tctgcccgat actacgccga ggccgcgcgc aatccgtggc ggccgagcca cgaccgctcg
      481 ccccaggtgg aggcgccggc gggggccacc tacctgggag gggacgtggg tcgtggaacg
      541 attccggccg tgcgggaggc gttcgacctg cgctacgaga actcacatcc cgtgggcggc
      601 cacttcgccc cgatggagga gccggaggtc gtgatcaacg acatccggga gacgttccgc
      661 ccgttgcggt gactttcgcg cggtcgatgg cggggacggc tagccgtcca tgatcagcgg
      721 gtcgtccagg gcgacgcctc gccgggcggc tgcgtgcagg gcgggaacct tcatgccggg
      781 cccgggctcc accagctcga tggtcttgcc gtcggggtcg cgcatcacgc agaacttgga
      841 gccggcgatc tgaccttcct cggccagcat gggcggcgtg tgaaagtcga tgccctcggc
      901 gctgagacgg gcgtagacat cgtcgagatc gtccacggcg aagcagatgt aaacatagcc
      961 gacgtcatcc tggcgcatgt ccatctgccg gcgggtcggt tcgaactcca gcagctctat
     1021 ggcgaaatcg ccgacgggca gcagcgcggc gcgatgtatg tggggcggaa gccgcagcac
     1081 ctgctcgccg gtggggcctg aaacttccag cgctaccccg ggcttcagcc cgaacacctt
     1141 gctgtaccac tcaacggcgc gctccagatt gctcaccgtg atgccgacgt ggtgaaaggt
     1201 tgctgatatc ggcagggtca tgggagcgcg gcccaggagg cctttgcctc ttccagcgtg
     1261 atcacgtcgg cgggatcgag tttctgcagc gtgctgcccg gctcgtcgat gtagcagatc
     1321 tcgccttcca gcagcatgcg cccgtcgatc tcacggaacg gaaagaacca ggaaaggtgc
     1381 gcgtgagaca gataccaggc gtcacgatct tccacctgct ggccgttggc gatgagcgtt
     1441 tcacccggca cctgctggta ccactcgccc tcgcaggcca cgccccagtc cgcggccgcg
     1501 caccggtgga tggcgagctc caccaggctg ctgcgcgtgt tccagatgtt gtagtagaac
     1561 tcccggacct cttccatgcc ggtgagcacg gcgttgtcga agctcgcgta gaaacggtat
     1621 tcggggtcgg gaaccatgga atcgaagatc gcagggtcgc cgaggcattc gtatttcagg
     1681 tggtgccgca tgttgtcgag catggccttg cggcgcgggt cggtctccgc gttcatcagc
     1741 tccgtgtagc ggtgccagcc cttcagcggt tcgatgcgca tctcgtctcc ccattcttaa
     1801 cgatcgagct gcttaagtta tcacgatttg tcggcgtctc tgcctgccgg aggccggcgt
     1861 cgggcgatgg tcctcaccag acgcccggga gcaggcggta acggacgcgc cgggcgtagt
     1921 cggcgtagcc gggaagctcc cgctgcagcg tccggtcctc cagggccgtt cgcaccacca
     1981 tgccgagtgc tgagatggtg gcgggaacga acgcccacca ggaacccagc agcagggggg
     2041 ccgagaagca ccagccgaag aagccgacgt agccgggatg ccggatgatc tggtaagggc
     2101 cggcgtcgac gacccggtgg cctcgctccg tctggatccg gacggtcttc tcgaagaaag
     2161 ggttgacgcc cattgaccag ctgaacagtg acgtccccgg cacaaacacg gcgagaccca
     2221 gccaccacag ccggtcgctc atggccgtcc actcgtagcg aacggcgtcc aagcccgcga
     2281 cgacgtagat cgagcagaac gccggcgcga agcacacgct ccagatcacg tcccagcgtt
     2341 ttgtgccggc gccgaaacgc gcgcgcgccg cgatcaggga gggattcacg cgctgcagat
     2401 acacgagatt gacgacgaaa ttggcgccca cgagcgcaag gtagccccac gcccggggcc
     2461 aggcgaaggt gccggcaggc cagaagacga ggcccgccag cgccgcgctg ccggcgaacg
     2521 tggcggcgat cgcggcgacg agcactttcc cgatctgccc tggggaccct gctggaactg
     2581 gatggtttgc caagcgctcg atatcaagcc ggtgatcgat cgggcccgac gttaacccag
     2641 ctgccggcca tagccaaacc catctccctg gaagtcgacg gcccgtggga ggacgtcccg
     2701 ccgacgcgcg atgatggtgt cgcgagcagc gctggggtaa tctgcgccgc gccagtttca
     2761 gagtatcgtg atggttcgcg cattgttcgc ccgggagctc cgggtttagg gccggttggc
     2821 gggaagccga cattgtcacg aaaaagaacc ggccaccgcg ggagccaacc ttactcgtcc
     2881 gatccccaga ccgagcggtc gctgcgccat tccatcaagg acggcgttta cttctccgcc
     2941 atgaccgggg gggcggagag ctacttctcg gcattcgcca ttcatctcaa tgccagcacg
     3001 acgatcatcg ggttgctcgc ctcgctgcca ccgctgctcg catcattcat gcagatgctt
     3061 tccgcctggc tcggccgccg caccggccgc cgcaagccga tcatcgtagc gggtgccctc
     3121 ctgcaggcgg cgctgcttgt gcccatcgcg gcggtcccgc tgctctggcc cgatgccgcc
     3181 gcggtgctgc tgattcctct ggtgttcgtc tacctgtgtg gaccaaacct cggcgcaccc
     3241 caatggaaca gcctcatcgg tgatctgctt ccggaggccc gccgcggccg tttctttgcc
     3301 ctgcgcaccc ggttgtccag cgttgcctcc ctggccgcgc tggtgatcgc cggtctcgtc
     3361 ctcgaggcgt tcgacaagtc cggagacgcc tactgggggt tcgtggtgat cttcctgctc
     3421 gcgggcgctg cgcgctgcgt ctcggcctac catctcaatg ccatgacaga gccgacgcgc
     3481 aacgtggcgg ggcttctggc gccggacgac ctttcggtgt ggcggcgggt ccggcactct
     3541 agtctgctgc gtttctcgct gttcttcgcc gcgatgcagt tcgcggtggc gatatcaggg
     3601 ccgtacttca cgctctacat gctcagggat ctggcgctga gctacgtcga gttcatggtg
     3661 ctgagcttcg tgtcggtcac gatccagttc ctcaccctga accgttgggg gcggctggcg
     3721 gaccttttcg gcaaccggct gatcctgatt accac
//
LOCUS       DBNHKV010000052         2959 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_8224, whole genome shotgun sequence.
ACCESSION   DBNHKV010000052 DBNHKV010000000
VERSION     DBNHKV010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_8224"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /gene="sufB"
                     /locus_tag="AC16PP_01545"
     CDS             complement(<1..1200)
                     /gene="sufB"
                     /locus_tag="AC16PP_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIR6480335.1"
                     /translation="MSERTVHDLVQSEYSAGFTTDVESETLPPGLDESVVRFISQKKE
                     EPEWLRDWRLEAYAGWRQMTPPEWAHVHFPPINFEAISYYSAPKSQGDGPKSLDEVDP
                     ELLETYEKLGIPLHEQEMLAGVVKDPDSVARPRVAVDAVFDSVSIATTFKDKLAEAGV
                     IFCSISEAVHEHPELVQKYLGSVVPRRDNYYAALNSAVFSDGSFVYIPKGVRCPMELS
                     TYFRINARNTGQFERTLIIAEEGSYVSYLEGCTAPMRDENQLHAAVVELVAMDDAEIK
                     YSTVQNWYPGDKDGKGGIYNFVTKRGVCLGDRAKISWTQVETGSAITWKYPSVVLRGH
                     DSVGEFYSVALTNNFQQADTGTKMIHIGRNTRSTIVSKGISAGRSQNTYRGLVRTTPT
                     ATGARNYT"
     gene            1390..2022
                     /locus_tag="AC16PP_01550"
     CDS             1390..2022
                     /locus_tag="AC16PP_01550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021788.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6480336.1"
                     /translation="MPKLEQLPEPNIYQAYGIYLLESKHRLIRRLKKAYQPSVHGHKT
                     WKASFLLMDYLQHRPIAKKSRVMEIGCGWGPGSIFCAKRFKARVTAVDVDREVFPFLE
                     VLAELNDVKLKPLVSRFEKLDADCLGRQDVLIGSDVCFWDNMVKPLSRLINKSLDSGV
                     QRVILTDPGRPTFYEVCDLAAKRHKVELNSWYATEPDYCSGEVVEIRPKK"
     gene            complement(2055..2729)
                     /locus_tag="AC16PP_01555"
     CDS             complement(2055..2729)
                     /locus_tag="AC16PP_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480337.1"
                     /translation="MMGFHRIIALAALAAVATVLGGCVTATVQEVRESRTGMDASDAV
                     VVLGRRSRPKSAETELDFVTCISRNLSKGAAGIKVIEEQQFMDSLFPWFEPRTAPVKT
                     ADLPELIALPPLARRLEEMNLRYLVWVEGSTERTDSAGSLTCSVTATGAGCFGFLTWE
                     NDSSYEAFVWDVRNGRVAGKLSSDAVGTSYVPAIVVPVPFIARVRSSACSSLANQLKS
                     FLANEA"
     gene            complement(2726..>2959)
                     /locus_tag="AC16PP_01560"
     CDS             complement(2726..>2959)
                     /locus_tag="AC16PP_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480338.1"
                     /translation="IRYQFQLYDGEGQLLADWPLTAYGKADEDNYGLSTDQPALQAAA
                     LAACRDAMAFFTVQFRDVPSVKAWLDRQLGGTS"
BASE COUNT          550 a          861 c          937 g          611 t
ORIGIN      
        1 cgtgtagttg cgcgcgccgg tggcggtcgg cgtggtcctg accaggccgc gataggtgtt
       61 ctggctacgg ccagcactga tgccctttga gacgatcgtg ctgcgggtat tgcgaccgat
      121 gtggatcatc ttggtgccgg tgtcggcttg ctggaagttg ttggtcagcg caaccgagta
      181 gaactcgccg acgctgtcat ggccgcgcag caccacgctc ggatatttcc aggtgatcgc
      241 cgaacccgtt tcgacctggg tccaggagat cttggcgcgg tcgccgaggc agacgccgcg
      301 tttggtcacg aagttgtaga tgccgccctt gccgtccttg tcgcccggat accagttctg
      361 cacggtcgag tacttgatct cggcgtcgtc catggcgacc agctcgacga cggcagcatg
      421 cagctgattc tcgtcgcgca tgggggccgt gcagccctcc agatagctca cgtatgaacc
      481 ttcctcggcg atgatcagcg tgcgttcgaa ctggccggta ttgcgtgcgt tgatcctgaa
      541 gtaggtggaa agctccatgg ggcagcgcac gcctttgggg atgtaaacga aggagccgtc
      601 gctgaacacg gcggaattca gggccgcgta gtagttgtcc cgtcgcggaa ccacgctgcc
      661 caggtatttc tggacgagct cgggatgttc gtggacggct tcggatatcg agcagaagat
      721 gacgccggct tcggcaagct tgtctttgaa ggtggtggcg atggagacgc tgtcgaacac
      781 cgcgtcgacc gcgaccctgg gtcgcgcgac cgaatcaggg tccttgacga ccccggccag
      841 catttcctgc tcgtgcaggg ggatgccgag cttttcgtag gtttccagca gctcggggtc
      901 gacctcgtcc agggatttgg gaccgtcgcc ctggcttttc ggtgccgagt agtaggaaat
      961 ggcttcgaag ttgatgggcg ggaagtgcac gtgagcccat tcgggaggcg tcatctgacg
     1021 ccaaccggca taagcttcca ggcgccagtc ccgcagccac tccggttctt ccttcttctg
     1081 ggagatgaat ctgaccacgg attcgtcgag ccccggcggc agggtctccg attcgacgtc
     1141 ggtggtgaat ccggcgctgt attctgactg cacgagatcg tgcacggtcc gttcagacac
     1201 ggtgaaaatc gcttaaggtg aaagagcgca attgtacccg ctgagggtgg cggaatacca
     1261 gagtaaaata gtcgggtatt tggggtccga tgactcgccc gtcttgaatt ccggtttact
     1321 gccgcgaatc ccgcacagta acatccgcgc tcttttgtcg cgcttcattc gcaggaaccg
     1381 atcctcatca tgcccaagct cgagcaactt ccggagccga acatctatca ggcttacggg
     1441 atctacctac tcgaatccaa gcatcggctc attcgtcggt tgaaaaaggc ttaccagcct
     1501 tcggtgcacg gacacaagac ctggaaggcg agctttctgc tcatggacta tctgcagcat
     1561 cgccccatcg ccaagaagtc ccgggttatg gagatcggct gtggctgggg ccccggttcc
     1621 atattctgtg ccaagcgttt caaggcccgg gtgactgcgg tagacgtcga ccgtgaggtc
     1681 tttccctttc tcgaggtgct cgccgagctc aatgacgtca agttgaagcc tctggtgagc
     1741 cgcttcgaga agctggatgc cgattgcctg ggcaggcaag acgtgctcat cgggtccgac
     1801 gtctgtttct gggacaacat ggtcaagccg ctttcgaggc tcatcaacaa gtcgttggat
     1861 tccggggtgc agcgagtgat ccttaccgat ccggggcgcc ccaccttcta cgaggtgtgc
     1921 gatctcgccg ccaagcggca caaggtcgag ctgaacagct ggtatgctac agagcccgac
     1981 tactgctcag gagaagtggt cgagatccgg cctaaaaaat aataaaaaca gatagttatg
     2041 aggcttaact agaatcatgc ctcgttagcc aggaaggatt tgagctggtt ggcgagactc
     2101 gagcaggccg acgatcggac ccgggcgatg aagggcacgg ggacgacgat ggcgggcacg
     2161 tagctggtgc ccacggcatc cgagctgagc ttgcctgcga ccctgccgtt gcgcacatcc
     2221 cacacgaacg cctcgtaaga ggaatcgttc tcccaggtca ggaatccgaa gcagcccgca
     2281 ccagttgcgg tcacgctgca cgtcagcgaa ccggcagagt cggtccgctc ggtggatccc
     2341 tccacccaga cgaggtagcg caggttcatt tcttccagcc gtcgggccag gggcggcagc
     2401 gcgatcagct ccggcagatc ggccgtcttc acgggcgctg tgcgtggctc gaaccatggg
     2461 aacaggctgt ccatgaactg ctgttcctcg atgactttga tgccagcagc ccccttgctg
     2521 aggttcctgc tgatgcaggt gacgaagtcg agctccgtct cggcagattt aggtcggctg
     2581 cgacgcccga gcaccaccac ggcgtcgctg gcatccattc ccgttctgga ctccctgacc
     2641 tcctgaacgg tggcggtgac gcagcccccg agcacggtgg cgacagccgc gagcgctgcg
     2701 agggcgatga ttcgatgaaa acccatcatg aggtgcctcc cagttggcgg tcgagccagg
     2761 ctttgacgct cggcacgtca cggaactgca cggtgaagaa ggccatggcg tcccggcagg
     2821 cggccagcgc cgcggcctgc agggcgggct gatccgtcga gagcccgtag ttgtcctcgt
     2881 ctgcttttcc gtaggccgtg agcggccagt cggccagcag ttgcccttcg ccgtcgtaca
     2941 gctgaaattg atagcggat
//
LOCUS       DBNHKV010000053         3164 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1920738, whole genome shotgun sequence.
ACCESSION   DBNHKV010000053 DBNHKV010000000
VERSION     DBNHKV010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1920738"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="AC16PP_01565"
     CDS             <1..737
                     /locus_tag="AC16PP_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6480339.1"
                     /translation="PGFGADVCRNMRLFISGSAPLTEATFHAWRERTGFEILERYGMS
                     ETIIIASNPLNGERLAGTVGFALPGMEVRIADEEGKPLSGDDVGMIEVRGSSVFSGYW
                     RMPEKTAEEIRPDGFFVTGDLGRMDADGRVTIVGRSKDLVISGGYNVYPKEVERLIDE
                     LPGVVECAVIGVPHPDFGEAVVAVVVPENEEVSERSVAAGLDGQLARFKQPKRVINIG
                     ELPRNAMGKVQKNELRERFADLFAQS"
     gene            complement(734..2428)
                     /locus_tag="AC16PP_01570"
     CDS             complement(734..2428)
                     /locus_tag="AC16PP_01570"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR6480340.1"
                     /translation="MEIDLWRPLAGLSLFLFSMSIIERALEDVSGRAFRRFLRRNTDH
                     PIRGIGSGTLATAILQSSSLVGVMMLALVGAGVIQMRNALSVIFGANLGTTMTGWIVA
                     TIGFKLDLDALALPLIAAGGLAAMGIRDHRASQIARVVFGVGLLLMGLDLMKGAVGNI
                     SDAIDVAVLGELGAVEYLLFGLVFTAIVQSSSATMMVTLSALHGGIIDLPAAAAVVIG
                     ADLGTTGTVLLAALRGSAAKKRVAMGHFLFNLITDLTAFALRTPLLAVILMIGLSDLL
                     ALVAFHSAFNLIGILMFLPLIGRFAGWLEGRFTDTETSVSRHLADVAVDLPEAAVEAI
                     RLETNHLMQRVICQNLKVPDPEISLPAGSLPVGVHEAGAIAAALEPEFEAAYAASKRL
                     EGEIITFAADAQAASLEKSESDLITRCQFAAREAVHASKSLKDARGDLRAFALSHRPI
                     LNGYGNRFREIMSEFYTRLFLLRHGPTAERADGGPELEDLIVLNQLAHQRHEELHTAI
                     YEDVRADRLDEDEISSLLNANRELFNSNRALLSAIATITLPPEQAATFDLLPTSVV"
     gene            2472..3080
                     /locus_tag="AC16PP_01575"
     CDS             2472..3080
                     /locus_tag="AC16PP_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR6480341.1"
                     /translation="MTWLLVSNLLLLAVLGALALVVLSLARQIGILHERTAPLGLVSK
                     SQGVEEGGRVPDLELPTLTAAHVGLKDAADAGRWSALLFIAAECPICRSVLPAYLDML
                     RDREPWLEGYWVSDGSDMPAYPAYAQANGIDLERYLISQELALHLQVQQLPSIAIIDD
                     QGRLAAGRLVQSARQLHELFQRIDADRHRQPEQEGTYVHSHE"
BASE COUNT          581 a          935 c         1068 g          580 t
ORIGIN      
        1 tcccgggttt cggtgcggac gtctgccgca acatgaggct cttcatctcc ggctctgcgc
       61 cgctcaccga agcgaccttt cacgcgtggc gtgagcggac gggtttcgag attctcgaac
      121 gctatgggat gagcgaaacc atcatcatcg cgtcgaaccc gctgaatggc gaacggcttg
      181 ccggcacggt gggttttgcc ctgccgggca tggaggtgag gatcgccgac gaggagggta
      241 agccgctgtc tggagacgac gtgggtatga tagaggttcg cggatcgagc gtgttttcgg
      301 gttactggcg catgcccgag aaaacggcgg aggagatccg tccggacggg tttttcgtta
      361 ccggggatct tggccggatg gacgcggacg gtcgcgtcac gatcgtgggc cgatccaagg
      421 acctcgtcat ctccggtggc tacaacgtct atcccaagga ggtcgagcgt ctgatagacg
      481 agcttccagg cgtggtcgag tgcgccgtca tcggcgtgcc gcatccagac tttggcgaag
      541 ccgtggtcgc cgtggtcgtt ccggagaacg aagaggtgag cgagcggtcg gtagcggcgg
      601 ggctggatgg ccagctcgct cgattcaagc agcccaaacg cgtgatcaac atcggcgaac
      661 ttccccgcaa cgccatgggc aaggtgcaga agaacgagct ccgagagcgt ttcgccgacc
      721 tcttcgcgca aagctagacc acgctggtcg gcagcaggtc gaacgtggcc gcctgctccg
      781 gcggcagggt aatggtggcg atggccgaaa gcagcgcccg gttcgagttg aacagctcac
      841 ggttggcatt gagcagcgag gagatctcgt cctcgtccag ccggtccgcc cggacgtcct
      901 cgtagattgc cgtgtgcagc tcctcgtggc gttgatgagc gagctggttg aggacgatca
      961 gatcttcgag ctccggccct ccgtccgccc gttctgccgt cggcccgtga cgaagcagga
     1021 agagtcgtgt gtagaactcg ctcatgattt cacgaaagcg gttgccgtag ccgttcagaa
     1081 tgggccgatg ggagagggcg aacgctctga ggtcgcctct cgcgtccttg agactcttcg
     1141 atgcgtggac ggcttcgcgg gcggcaaact ggcatcgcgt gatgaggtcg ctttcgctct
     1201 tctccagtga tgccgcctgg gcgtccgcgg caaacgtgat gatctcgccc tcgaggcgtt
     1261 tgcttgccgc atatgccgct tcgaactcgg gttccagcgc ggcggcgatg gcgccggctt
     1321 cgtgcactcc caccggtagc gaacccgcgg gcaacgatat ctcgggatcc ggcaccttta
     1381 ggttctggca gatcacccgt tgcatgaggt gattggtctc caggcggatg gcctctactg
     1441 cggcctcggg cagatcgacg gcgacatcgg cgaggtggcg gctcaccgag gtctcggtgt
     1501 cggtgaaacg tccttcgagc caaccggcga agcgaccgat caacggcagg aacatcagga
     1561 tcccgatcag attgaacgcg ctgtggaagg cgaccagagc gagcagatct gacaacccga
     1621 tcatgaggat cacggccagc aacggcgttc gcagcgcgaa ggccgtgaga tcggtgatca
     1681 gattgaacag gaagtggccc atggcaaccc gttttttggc ggcgctgccc ctcaaggccg
     1741 ccagcagcac ggtacccgtg gtgccgaggt cggccccgat gaccactgca gctgccgcag
     1801 ggaggtcgat gatgccgccg tgcagcgcgc tcaaggtcac catcatggtt gccgagctgg
     1861 actggacgat ggcggtgaag acgagcccga agaggagata ctccaccgcg ccgagctccc
     1921 cgagaacggc aacgtcaatg gcatccgata tgttgcccac ggcgcccttc atcaggtcga
     1981 ggcccatgag cagcagaccc accccgaaga cgacgcgggc gatctgggat gcgcgatgat
     2041 cgcgaatgcc catggcagca agaccgccgg cggcgatgag gggcagcgcc agcgcgtcga
     2101 ggtcgagctt gaagccgatg gttgccacga tccacccggt catggtggtg ccgagattgg
     2161 caccgaatat cacagacagg gcgttgcgca tctggatcac gccggcgccc accagggcga
     2221 gcatcatcac gccgaccagg gagctcgact gaaggatggc cgttgccagc gtgccgctgc
     2281 cgatgcccct gatgggatgg tctgtgttgc ggcggagaaa tctgcggaag gctcggccgg
     2341 agacgtcctc taaagcgcgc tcgatgatgc tcatggagaa caggaacagg cttagccccg
     2401 ccagcggacg ccagaggtcg atttccatgg cggtatcgta ccggaggcgg cgtggattac
     2461 actccctggt catgacttgg ctgttggtct caaacctcct gctgttggcc gttctgggtg
     2521 cccttgcgct ggtggttctc tcgcttgccc gtcagatcgg cattctccac gaacgcacgg
     2581 cgccgctggg tctcgtgtcg aagagccagg gtgtggaaga gggtggccgt gtgcccgacc
     2641 tcgagttgcc gacgctcacg gccgcccatg tcgggctcaa agatgcggcc gacgctgggc
     2701 gatggtcggc gttgctcttc atcgccgccg agtgtccgat ctgtcgctcc gtgctgcccg
     2761 cctacctgga catgctgcgg gatcgcgagc cgtggctgga gggttactgg gtgagtgatg
     2821 gttccgacat gcccgcctac ccggcttacg cccaagccaa cggcatcgac ctggaaaggt
     2881 acctgatttc ccaggagctg gccctgcacc tgcaggtgca gcagctgccg agcatcgcaa
     2941 tcattgacga ccagggcagg ctggcagccg ggcgcctcgt gcagagcgcc cgacagctgc
     3001 acgaactatt tcagcggatc gacgctgatc gtcacagaca acccgagcag gagggcacct
     3061 atgttcatag ccatgaatag atttcagatc gccaaagggt tcgaggaggg attcgaaaaa
     3121 gtctggcggg agcgggaaag ctttctgtcg gacgtgccgg gctt
//
LOCUS       DBNHKV010000054         4156 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_79914, whole genome shotgun sequence.
ACCESSION   DBNHKV010000054 DBNHKV010000000
VERSION     DBNHKV010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_79914"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="AC16PP_01580"
     CDS             complement(<1..354)
                     /locus_tag="AC16PP_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480342.1"
                     /translation="MAHEPISRTLAVAVAIALVCSVMVSTAVYLLRPIQIAYDLIDRN
                     RAIVAAAGIEGVLQGGDDAAVVRAFLDLDARVLDLDSGEFLDTTDAHGYDHWAGTTDD
                     ARLVPVYVSRRDGAAT"
     gene            complement(357..1502)
                     /locus_tag="AC16PP_01585"
     CDS             complement(357..1502)
                     /locus_tag="AC16PP_01585"
                     /inference="COORDINATES: protein motif:HMM:NF015100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR6480343.1"
                     /translation="MADSTILQRVRGIVIDLADPYRAPEARAAGSPPFLRQALDTPGL
                     LACFVVATLPAWAVGVWNTGRRGLEAGFGSEGESWQLGMLAAFRVDADPTAMLACLTL
                     GVLYFIPLLAAVLATSAFWALVFARTRQGGVDPGWLFSGWLMVLLMPPSTPLTIAVLA
                     ASFGVVLGSHIFGGTGKYLISPALLGVLFLHFSYPELVQTPLAAGDAGSADGWRALAA
                     GGEDVVEFLLGREPGGLATGSALACAVGALYLVRVGAASWRTIAGAVVGFLVAATVVN
                     VSAGEDPAWHVPWYAHLAVGYVAFGVAFLATDPSAAPLTRPARWAHGLLIGVLTVVIR
                     VLDPTHPEGSLYALLLAGLSVPFLDYLVVRRYRTRVRIRAQLRTRGL"
     gene            complement(1502..2773)
                     /locus_tag="AC16PP_01590"
     CDS             complement(1502..2773)
                     /locus_tag="AC16PP_01590"
                     /inference="COORDINATES: protein motif:HMM:NF048463.2"
                     /note="uses the energy from reduction of ubiquinone-1 to
                     ubiquinol to move Na(+) ions from the cytoplasm to the
                     periplasm; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit A"
                     /protein_id="HIR6480344.1"
                     /translation="MLIEITTGLDVEVSGRPEQTIDTGPEVGQVALLGGDYLNLRPSL
                     EVDEGARVKAGDTIFSDRARPEIRFVAPAAGRITQINRGPRRTLVSVVIDVDGDERAI
                     VDVPDDPSRREEIVALLLRSGLWPALRSRPFGRVADPGGTPAALLLTAIDTRPLAPDP
                     AVVVGLREADFAAGARALACLAPVTYLCSRAGASLPDVDAEGLSVVEFSGPHPAGLVG
                     THLRHLCPEADEVWHIGYQDVLALGRLLVSGELCDERVVSVAGPGAVRPRLLRTRAGA
                     RLSELVQGESENGCAVIAGSVLSGRAPTPASGYLGRYDSQVSLLRAAGPSRRRWLDVV
                     WPRGAAPARNIGRGSGMLPVESFDRVWPFQAPVAPLLRALLTGDTELASSLDCLSLVE
                     EDLALCAYVCPSRLDYGAALRATLHEIERGR"
     gene            2863..3711
                     /locus_tag="AC16PP_01595"
     CDS             2863..3711
                     /locus_tag="AC16PP_01595"
                     /inference="COORDINATES: protein motif:HMM:NF025890.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR6480345.1"
                     /translation="MRLFSMAVGTLFLLATGQVGGNPTCAACHGELVADFHGTPHGAR
                     TEDDEASGCRSCHGDPATHLAAPAEPGSLLAFDDEPSATINAACGSCHADAHTARANP
                     HSQAGLACTDCHSIHPSSAAEIALPAEFDRVDAASATCFECHQETFSQFSFNERHRLQ
                     EGSVSCTSCHDPHAPVSSGHLGAFGQRVCAECHADVDGPFVFEHDASRVEGCGACHEP
                     HGSPNRHMLTHQQVGELCYSCHAIVPQFHLGFSPAGPPRFDEGTVCTNCHATIHGSNL
                     DPAFLR"
     gene            3719..>4156
                     /locus_tag="AC16PP_01600"
     CDS             3719..>4156
                     /locus_tag="AC16PP_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480346.1"
                     /translation="MTARLLIVFTLLIAGAASTRSAAAATFTGHVSVGYREVDVSGDE
                     GKYQQHVNLDDGARLFDLGMVFQPDRDANSPAGMPDRVELNASGLGGDPYESVRVRVQ
                     RYGSYRLTYDHRSSDYFYEDLLLAPEEASVEQSTGGDFHHFDFE"
BASE COUNT          756 a         1491 c         1333 g          576 t
ORIGIN      
        1 ggtcgctgcg ccgtctctcc tcgagacgta tacgggaacg agccgcgcgt cgtccgtcgt
       61 gccggcccag tggtcatagc cgtgagcgtc ggtcgtatcc aagaactcgc cgctgtcgag
      121 atcgagcacc cgggcatcca ggtcgagaaa cgctcgaacg acggcggcat cgtccccgcc
      181 ctgcagcacg ccctcgatac cggcggccgc gacgattgcg cggttgcgat cgatgaggtc
      241 gtaggcgatc tgaatcggcc gaagcaggta gacggcggta gaaaccatga ccgagcagac
      301 cagcgctatg gccacagcga cagccagcgt gcgggaaatc ggttcgtgcg ccacgatcag
      361 aggccccgcg tccgcagctg cgcccgaatc cgcacccgcg tgcggtacct ccgcaccacc
      421 aggtagtcca gaaacggcac gctgagcccc gccagcagga gcgcgtacag agagccttcc
      481 gggtgggtcg gatccagcac gcggatcact accgtgagca cgccgatcag cagaccgtgc
      541 gcccaccggg ccggccgggt cagtggcgca gcgctgggat cggtggccag aaaggcaacc
      601 ccgaaagcca cgtagccgac cgccagatga gcgtaccagg gcacatgcca ggcggggtct
      661 tcccccgccg agacgttgac gacggtggcc gcgacgagga agcccacgac ggcgcccgcg
      721 atcgtgcgcc aggaggcggc gcctacgcgc accagataga gcgctccgac ggcgcaggcg
      781 agcgcggagc cggtcgccag gcctcccggc tcgcgcccca ggaggaattc gacgacgtcc
      841 tcgccgcccg cggccagcgc gcgccacccg tctgcgctcc ccgcatcgcc agcggcgagc
      901 ggggtctgta ccagctcggg atagctgaag tgcaggaaca gaacgccgag cagggccggg
      961 ctgatcaggt atttccccgt cccgccaaag atgtgactac cgagcaccac gccaaagctc
     1021 gccgccagga ccgcgatggt cagcggggtc gacggcggca tgagcagcac catgagccac
     1081 ccgctgaaca gccagccagg gtcgacgccg ccctgccggg ttctcgcaaa aaccagggcc
     1141 cagaaggcac tggtggccag gacggccgca agtagcggga tgaaatacag caccccgagc
     1201 gtcaggcagg ccagcatggc cgttggatcc gcatcgacgc ggaacgcggc cagcatgcca
     1261 agctgccagc tttcgccctc gctcccgaac cctgcttcca gtccgcgccg accggtgttc
     1321 cagacaccga cggcccaggc cggcagggta gccactacga agcaggcgag cagtcccgga
     1381 gtgtccaggg cctggcgcag aaagggcggc gatcccgccg cgcgggcttc aggtgcgcgg
     1441 tagggatccg cgaggtcgat cacgattccc ctgacccgct gaaggatcgt cgagtcagcc
     1501 atcagcggcc ccgctcgatc tcgtgcaggg tcgcacgcag cgcggccccg tagtctagtc
     1561 gcgatgggca cacgtatgcg cacagggcca ggtcttcctc gaccagagac aggcaatcca
     1621 gggagctcgc gagctcggtg tcacccgtga gcagcgcacg aaggagcggc gcgacggggg
     1681 cctggaacgg ccagacgcgg tcgaagctct cgacgggtag catcccgctc ccacgaccga
     1741 tgttcctcgc cggagcggcc ccgcgcggcc aaaccacatc cagccaccgg cgtcgtgacg
     1801 gacctgccgc gcgcagcagc gatacctgag aatcgtaccg gccaagatac ccactcgccg
     1861 gcgtcggtgc gcgtccgctc agaacggacc cggcgatcac ggcacagccg ttttccgact
     1921 cgccctgcac aagctcgctc aggcgcgcac ccgcccgcgt gcgcagcagc cgcggcctga
     1981 ccgctcccgg cccggccact gaaaccactc gttcatcgca aagctcgccc gagacgagca
     2041 atcgtccgag tgccagaacg tcctgatacc cgatgtgcca gacctcgtcc gcctccgggc
     2101 acaggtggcg gagatgggta cccaccaggc cggccggatg cgggccgctg aactccacca
     2161 cgctcaagcc ctcggcatcg acgtccggga ggctggcccc tgcccgggag cagagatagg
     2221 tgaccggcgc cagacaggcg agcgcccggg cgccggcggc aaagtcagcc tctcgaagcc
     2281 cgacgaccac ggccggatcc ggggccaggg gccgggtgtc gatggccgtg aggagcagcg
     2341 cggccggtgt ccctccggga tcggccacgc gaccgaaggg tcgcgatctg agggcgggcc
     2401 aaaggcccga acgcaggagc agcgcgacga tctcctcgcg ccgtgacggg tcatccggca
     2461 catcaacgat cgcccgctca tccccatcga cgtcgatcac caccgagacc agcgtgcgcc
     2521 gtggtccacg attgatctgg gtgatccggc cggcggctgg tgctacgaag cgaatttccg
     2581 gacgcgcccg gtcgctgaag atcgtatcac ccgctttgac ccgggcacct tcgtccacct
     2641 cgagcgacgg gcgaaggttc aggtagtccc caccaagcag ggcaacctgg ccgacctccg
     2701 gacccgtatc gatggtctgc tccggccgac cggagacttc gacgtcaagg cccgtggtga
     2761 tctcgatcag catgccggca ccaaactgac acgaccctcg ggtagtatct ctacgtagaa
     2821 ctcacaatag cagagcgaca cgtgctgctg gaccctaaac ccatgcgttt gttttcaatg
     2881 gcggtgggca ctctgtttct gctggcaacc ggccaggtcg gcggaaaccc gacctgcgcc
     2941 gcttgccacg gcgagctcgt cgcggacttc cacggtactc cccacggggc ccggacggag
     3001 gacgacgaag cgtccggatg caggagttgc cacggcgatc cggcaactca cctcgccgca
     3061 cccgcggagc cgggatccct gctcgcattc gatgacgagc ccagcgcgac gatcaatgcc
     3121 gcctgtggca gctgtcacgc ggacgctcac accgctcgcg ccaacccgca ctcccaggcc
     3181 gggctcgctt gcacggattg ccacagcatt cacccatcga gcgcagccga aatcgctctg
     3241 cctgccgagt tcgatcgcgt cgacgcggcc tcggccacgt gcttcgaatg ccaccaggag
     3301 acattcagcc agttctcctt caacgagcgt catcgcctac aggaaggcag cgtttcctgc
     3361 acgtcctgcc acgaccccca cgcgccggtg agctccgggc atcttggcgc cttcggccag
     3421 agggtgtgcg ccgagtgcca cgccgacgtc gacggcccgt tcgtgttcga gcatgacgcg
     3481 tcccgtgtgg aaggatgcgg cgcctgccac gagccccacg gcagcccgaa tcggcacatg
     3541 ctcacgcacc agcaggtggg cgagctctgc tactcgtgtc acgccatcgt cccccagttc
     3601 cacctcggct tcagccccgc gggcccgccc cgattcgacg aaggcaccgt gtgcacgaac
     3661 tgccacgcca ccattcacgg gtccaacctg gacccggcct ttctgaggtg acgggctcat
     3721 gacggctcgg ctcctgatcg ttttcacgct gctgatcgcc ggtgcggcaa gtacccgcag
     3781 cgccgccgcc gcgaccttca ccggtcacgt gagcgtcggc taccgcgagg tcgacgtgtc
     3841 cggggacgag ggcaagtacc aacagcacgt caacctcgat gatggggccc gcctgttcga
     3901 tctcggcatg gtcttccagc ccgaccggga cgcgaatagc ccggccggca tgcccgaccg
     3961 ggtagagctc aacgccagcg ggctcggcgg ggacccctac gaatccgtgc gggtgcgcgt
     4021 tcaacgttac ggaagctacc ggctcaccta tgatcatcgc agctcggact atttctacga
     4081 ggacctgctg ctcgcacccg aggaagccag cgtcgagcag tccacaggcg gcgacttcca
     4141 tcatttcgac ttcgag
//
LOCUS       DBNHKV010000055         2153 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1496893, whole genome shotgun sequence.
ACCESSION   DBNHKV010000055 DBNHKV010000000
VERSION     DBNHKV010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1496893"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="AC16PP_01605"
     CDS             <1..1032
                     /locus_tag="AC16PP_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6480347.1"
                     /translation="QLKPPGMTGSSEDLRCPFHGWTWSLDGDLVDLPQAWDFPHVTED
                     SHRLPEVKVGLWGGFVFVNFDPDAAPLEDYLGVLPRHFADFHIEDRYIELHIRKRLPA
                     NWKASAEAFLEAYHVRETHAGGRPNTEVATQYDVFGDNVTRFIHTVGAPCPLVDPPPS
                     EPQLLEMLWVRGREDEPVPQVPEGTTARDFYADIVKTQFSERFGHDFSTMSTAQTLDS
                     IEYFVFPNMFLFPGLSLPMVYRFRPDPDDPDFCTFDLLFLRPKPKDRPAPPPPDPVDL
                     DVDDSYMLVEGTGRLGGVYDEDTVNLAAQTRGFKSSLKRGETLGNYQEIRARHLHRMV
                     NRYISGELP"
     gene            complement(1139..1888)
                     /locus_tag="AC16PP_01610"
     CDS             complement(1139..1888)
                     /locus_tag="AC16PP_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480348.1"
                     /translation="MRKILTSIAAATLLFGISLSAAAAPGWLATGLTVMPENAPKVVA
                     ALDALFDSKVGRKLPGRVILRANLADGNNPETHAIVSLFRSAAESEAYRAELWADPAW
                     GEFMAAMRGLNETAGSTMRGMIAYNSGERADSDVVWVNHYVTVSEPAVLLSAMRSYSQ
                     SATGQAAPGQVHLSAVTAGGVGSASHIISVGWASEAEWESEVQRTAGNPQWNALIRTM
                     RAVAEYHGATLQRDIKSWGKASVEEITNLGN"
BASE COUNT          334 a          718 c          705 g          396 t
ORIGIN      
        1 cagctgaagc cgccgggcat gaccggatcg tccgaggatc tgcgctgccc gttccacggc
       61 tggacgtggt cgctggacgg ggatctggtg gacctgcccc aggcctggga cttccctcac
      121 gtgaccgagg acagccatcg gctgccggag gtcaaggtcg gcctgtgggg cgggttcgtc
      181 ttcgtcaact tcgacccgga cgcagcgccg ctggaggatt atcttggcgt gctgccgcgg
      241 cacttcgccg acttccacat cgaggaccgc tacatcgagc tgcacatccg caagcgcctg
      301 ccggccaact ggaaggcgtc ggccgaggcg tttctggagg cttaccacgt tcgggagacc
      361 cacgccggcg gccggccgaa cacggaggtc gccacccagt acgacgtctt cggcgacaac
      421 gtcacccggt tcattcacac cgtgggcgcg ccgtgcccgc tcgtcgatcc gcccccgtcc
      481 gagccgcagc tgctggaaat gctctgggtc cgcggccggg aagacgagcc cgtgccccag
      541 gtcccggagg ggacgaccgc gcgggatttc tacgcggaca tcgtcaagac ccagttctcc
      601 gagcgcttcg ggcacgactt ctcgaccatg tcgacggcgc agacgctcga ttccatcgag
      661 tacttcgttt tcccgaacat gtttctgttc ccggggctat cgctgccgat ggtgtaccgg
      721 ttccggccgg acccggacga cccggacttc tgcacgttcg atcttctgtt tctgcggcct
      781 aagcccaagg atcggcccgc tccgccgccg cctgatccgg tggacctgga cgtggacgac
      841 agctacatgc tggtggaggg gacgggacgg ctgggcggcg tgtacgacga ggacaccgtt
      901 aatctggcag cccagacccg cggcttcaag tcgagcctca agcgcggcga gaccctgggc
      961 aactaccagg agattcgcgc gcgccacctg caccgcatgg tcaaccggta catcagcggc
     1021 gagctgccct gataccggca acctcgccag atgcgtatgc cggccaacgg cgccgtgacg
     1081 tagacgttac tcagacgaag gcaggcgccc ggcatgagcc gggcgccgaa ggcggacttc
     1141 agttaccgag attggtgatc tcttcgacag acgctttgcc ccaggacttg atgtcccgct
     1201 gcagggtggc gccgtggtac tcggccactg cccgcatggt tctgattagc gcattccatt
     1261 gaggattccc ggcggtgcgc tggacctcgc tctcccattc agcctcgctt gcccagccga
     1321 cggagatgat gtgggaagcc gaccccaccc cgccggcagt gaccgcgctg agatgcacct
     1381 gacccggagc ggcctggccg gttgccgact ggctgtagct gcgcatggcg ctcagcagaa
     1441 cggccggctc ggaaaccgtc acgtagtgat tcacccagac gacgtcggag tctgctcgct
     1501 cgccgctgtt gtaggcgatc atgccccgca ttgtgctgcc tgcggtctcg ttcagcccgc
     1561 gcatcgcggc catgaactcc ccccaggcgg ggtcggccca gagctcggcg cgataggcct
     1621 cgctctcagc cgctgatcgg aagagcgata cgatggcgtg ggtttccgga ttgttcccgt
     1681 ctgccaggtt tgcccgcagg atcacgcggc cgggcagctt gcgtccgacc ttggagtcga
     1741 acagcgcatc cagggccgcc accaccttgg gggcgttctc gggcatgacc gtcagcccgg
     1801 tggcgagcca tcccggtgcg gcggccgcgc tgaggctgat tccgaacagc agagttgctg
     1861 cggcgattga agtcaggatc tttctcatgg ttagtatttt cctttattga tctgcccctt
     1921 cccgaggcag gtgccggtca ctatagcgaa cgagagcgac gtttggcccg tcttttcgcc
     1981 cctgggccgg ggtatccgtt ccccgaagcg tccgttgccc tgcatgcatg cgccagggct
     2041 acccccgcag tggctgggta aggcccgcct ggtaagtcgt ctcgatgttg agctggcccg
     2101 ccaggccggc ggtgcggtgg gcggcgatct tcgcgtaggc ctcggaggtg gac
//
LOCUS       DBNHKV010000056         7244 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_433570, whole genome shotgun sequence.
ACCESSION   DBNHKV010000056 DBNHKV010000000
VERSION     DBNHKV010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7244
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_433570"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..520
                     /locus_tag="AC16PP_01615"
     CDS             107..520
                     /locus_tag="AC16PP_01615"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6480349.1"
                     /translation="MSGRSNDGGGREFRVTDVRVYRPYPDEVPWDLLDRQAPADPAGD
                     GVQMRIAKLGDHVVGAYVIEPVDPLTFRIVDLVVAEGWRRRGIGRWLLGHAIGLAESR
                     GAREVRVTNPSPRRFFASIGFLDADAGMRFPITPE"
     gene            complement(507..1667)
                     /locus_tag="AC16PP_01620"
     CDS             complement(507..1667)
                     /locus_tag="AC16PP_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR6480350.1"
                     /translation="MVADIIAALRCGPFANDKGANDMSSDTLPKGLEGVEVDETGISL
                     VDGERGVLSYRGIDVDELVDKPFDEVAALVATGRLDPDFGARLAASAELSEREAGLVR
                     SLPRDVHPMHVVQGLTPLLDRDGAFADFGDAAQGFAVAAKLPALIATHMLGTAVAGYP
                     TLGHAERFLAQIAAPQTDIARRAFNVTQILQIEHGFNASTFAARVAASTLAPVENALS
                     AGFGTLHGRLHGGADQAALEVAEQVGTAAAAAAFVDRCIADGTRVMGMGHREYRVLDP
                     RARHIKELARSLAAGTPHEKTFETLAAIEDRFRERMAEKGKALHANVEFYKGVVYRVL
                     GMTPAFFTTGFAMARVFGYLAHFIESREDNRLIRPKARYTGPPVGAVRLTPA"
     gene            1766..2647
                     /locus_tag="AC16PP_01625"
     CDS             1766..2647
                     /locus_tag="AC16PP_01625"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6480351.1"
                     /translation="MQIERHEIRIFSAVVEEGGFSRAAERLNITQSAVSQAVANLEHK
                     LDARLLERRGKPELTEAGKRLFAFTQAVIKEETNALDDIAQIRAGALSTLNLGMSSMF
                     NRLFGRELLLEFCERNPLTRLKLDVAPSRELIYGVDEDRWELGIGPFQTLMPGHFETK
                     RFFEEERVLVAHEKHPRFDALMSQPARELAQLTLLTSYLDDATKRPGSERLRNQFASV
                     WEISSLELRLTLAEAGMGVTYVSDRLVGDLEGFHPIDHMEISTIPRVVGIFHKKHVAL
                     SEGAKRFLAICQRRFAG"
     gene            complement(2658..3266)
                     /locus_tag="AC16PP_01630"
     CDS             complement(2658..3266)
                     /locus_tag="AC16PP_01630"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HIR6480352.1"
                     /translation="MNIQERMRAVLQAEAEAIAAVRIDETFEHAVRALLECRGKVITT
                     GIGKAGHIARKFAATLCSGATPADFIHPAEAAHGDLGLVGPDDVMIAFSTSGKSREVL
                     EILELARHLGLAMVIGITSHPDSELRDLSDLVLDMGEIEEPCPVGLTPSASTAVMLAI
                     SDAIALALMEAKGITKEDWGLRHHGGYLGRAARADNVPHDGG"
     gene            3400..5415
                     /locus_tag="AC16PP_01635"
     CDS             3400..5415
                     /locus_tag="AC16PP_01635"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012931.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004563 - beta-N-acetylhexosaminidase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HIR6480353.1"
                     /translation="MPYIAGPEGALLPLPRRFAPGPGGPGAVGVVDARFADASSARLV
                     RAVERLNDRLGRRNGEAGLKMALEISCAIPCPEHPQLGQPEAYQLNVTDQGARLVAET
                     EWGVLHGLETLFQLATAPGGLGCVAVDDAPRFPWRGLLLDVARHYLGMDALLRTLDAM
                     AQVKLNVLHLHLTDDQAFRFPSERFPELASPRHYSRRELAELINHAADRGIRVVPEID
                     VPGHTTCWLVARPDWGTGVTTSTRRFGVHEACLDPTSEAVYAALAELLGEVAEVFPDR
                     HLHLGGDEVHPAWWSASDRVQTFMSERGMTGLDDMQAHFNSRVARTVSGLGKRPMGWD
                     EVVHDDLPDDVVVQSWRGATARDRALGSGHDCVVSSGYYLDLFYPADVHYRFDPQAPE
                     SELVGLEDALLDDPRFEHVAEGMRWTEHWREPAGAAGAAPGSVLGAEACLWGELVTEE
                     LLDVRLWSRLPALAERFWSPRSVRDLGSLYQRLGSCFEQLAAGSGVDVRAASRALMAR
                     AGADEDVVELLELLEPVKWYGRLLGEEALAARIAGTEMPQARPYDADTPLDRVVDALF
                     PESLPARRVARLCQRHFEGDTGAVAGILDCALAWQDLGPRPDCPADVAPALELLCRVG
                     EAVVDVLEGCMAPSAALSLLETAARPQGEYLIAVVPPLRSWLAGLGG"
     gene            5412..6416
                     /locus_tag="AC16PP_01640"
     CDS             5412..6416
                     /locus_tag="AC16PP_01640"
                     /EC_number="2.7.-.-"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase enzyme family protein"
                     /protein_id="HIR6480354.1"
                     /translation="MIDPLQVARSGWRVGKEVDRLGAGHINDTYLVTDGVDRWVLQRI
                     NDQVFTDPALLMRNLGRVVEHVCARAPGFVPGLLSCRDGSNVFVDEEGGSWRLTEYVS
                     GTESHRRLESVDQATSAARAFARYQNLLADLPAPPLDDPIPGFMRLDGYLAALDAATA
                     RAAGEHGAEDLLGFVEGRRDLAQVLQDPGKTGGYIHGDCKINNLLFRENSSEVVCVVD
                     LDTTMHGHWAWDFGDLARSGAVTEAGALSLELFGALVEGFAGEKEAALGADELVLAPR
                     YVCFMLGVRFLTDHLDGDRYFKIAARGDNLRRARAQFRLLEEMEARESEMAEVAASRR
                     "
     gene            complement(6413..6658)
                     /locus_tag="AC16PP_01645"
     CDS             complement(6413..6658)
                     /locus_tag="AC16PP_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HIR6480355.1"
                     /translation="MSIPTEDDHDPDPPERGPLTLTQTVASALAAAFGVQSSRNRTRD
                     FSRGRPAQFIAVGVVLTALLILGIAALVHLVLAGIES"
     gene            6738..7133
                     /locus_tag="AC16PP_01650"
     CDS             6738..7133
                     /locus_tag="AC16PP_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6480356.1"
                     /translation="MLAGWRESPSGFAMTRCAALILVAILLCGCAVSSNRPTQLVSGS
                     AAYPEDARAQGIEGLVVVRYDVAVDGRVINARVVRAEPPDVFDDAALASVRSWRFNPA
                     LADGVPRATQDIESTVTFKLDGGDEYADY"
BASE COUNT         1079 a         2372 c         2563 g         1230 t
ORIGIN      
        1 tcttgcgccc ctgctgacgc gccggtacct cggagagagc gctaagacgg atcgcggcgc
       61 ccggcgatgg tggaagttct ggcaatgatt tggcgatgag ctggcaatga gcggtaggtc
      121 taatgacggt ggcggacggg aattccgggt aaccgacgtg cgcgtttacc ggccttatcc
      181 agacgaggtt ccctgggacc tgctggatcg gcaggcaccg gcggatcccg cgggagacgg
      241 ggtgcagatg cgcatcgcga agctcggcga ccacgtggtg ggcgcttacg tcatcgaacc
      301 cgtcgatccg ctgaccttca ggatcgtgga cctggtggtg gccgaaggct ggcgccggcg
      361 cgggatcggc agatggcttc tgggtcacgc catcgggctg gcggagtccc gtggggctcg
      421 cgaggttcgg gtgaccaatc catcccctcg gcggttcttc gccagcatcg gtttcttgga
      481 cgccgatgca gggatgcgat ttcccatcac gccggagtga gcctcactgc gcccacgggc
      541 gggcccgtgt accgagcttt cgggcggatc aggcggttgt cctcgcggct ctcgatgaag
      601 tgggccaggt agccgaacac ccgggccatg gcaaagcccg tggtgaagaa cgcgggcgtc
      661 atgccaagca cccggtagac gacgcccttg tagaactcca cgttggcgtg gagcgctttg
      721 cccttctcgg ccatccgctc gcgaaaccga tcctcgatgg ccgctaaggt ctcgaaggtc
      781 ttctcgtggg gcgtgccggc ggcgaggctg cgcgcgagtt ccttgatgtg gcgcgcgcgt
      841 gggtccagca cgcggtattc ccgatgcccc atgcccatca cgcgggtccc gtcggcgatg
      901 catcggtcca cgaacgccgc cgcagccgcc gctgtgccca cctgctcggc gacctccaac
      961 gccgcctggt ccgcgccgcc gtgcagacgc ccgtggaggg tcccgaagcc ggcggacagg
     1021 gcattttcca ccggtgcgag ggtgctcgcc gcgacccggg cggcaaaggt gctggcgttg
     1081 aagccgtgct cgatctgcag gatctgggtg acgttgaagg cgcgccgggc gatgtcggtc
     1141 tgcggggccg cgatctgggc caggaaccgc tccgcgtgcc ccaatgtcgg atagcccgcc
     1201 accgcggtgc cgagcatgtg cgtcgcgatc agcgcaggaa gcttggccgc cacggcaaat
     1261 ccctgagcgg cgtcgccgaa gtcggcaaag gccccgtcgc ggtcaagaag cggcgtgagt
     1321 ccctgcacta cgtgcatggg atgaacgtcg cgcggcagcg agcggaccag ccccgcctcg
     1381 cgctcggaca gctccgccga ggccgccagg cgcgcgccga aatcgggatc gagccggccc
     1441 gtcgccacga gcgccgcgac ctcgtcgaaa ggcttgtcga ccagctcgtc cacgtcgatg
     1501 cctcggtagc tcaggacccc gcgctcaccg tccaccagcg agatcccggt ctcgtccacc
     1561 tccacgcctt cgagcccttt gggcagggtg tcgctcgaca tgtcgttggc tcctttgtca
     1621 ttcgcgaacg gcccgcagcg tagcgcggcg atgatgtcgg cgaccatccg ctttcacttc
     1681 tccggagatc aggccggctt atccattagc ccagataatg gctctataag tggagcgccc
     1741 ccgcaggccc tggtagcctc cggacatgca gatcgagcgc cacgaaatca ggatcttcag
     1801 cgccgtggtg gaggaaggcg gcttcagcag ggccgccgag cgtcttaaca tcacccagtc
     1861 ggcggtcagc caggcggtgg cgaatctcga gcacaagctc gatgcccggc tgctcgaacg
     1921 ccgcggcaag ccggagctca cggaggcggg caagcggctg ttcgcgttca cccaggccgt
     1981 gatcaaagag gaaaccaacg cgctggacga catcgcgcag attcgcgccg gcgcgctttc
     2041 gaccctcaat ctggggatga gcagcatgtt caaccggctg ttcggccgcg agctgctgct
     2101 cgagttctgt gagcgcaacc cgctgacccg gctaaagctc gacgtggcgc cgagccgcga
     2161 gctcatctac ggcgtcgacg aggatcgctg ggagctcggc atcgggccgt tccagaccct
     2221 gatgcccgga cacttcgaga ccaaacgatt cttcgaggag gagcgcgtgc tggtggcgca
     2281 cgagaagcac ccgcgcttcg acgcgctcat gagtcaaccc gcccgcgagc tggctcagct
     2341 gaccctgctc acctcgtatc tggacgacgc caccaagcgc ccgggcagcg agcgccttag
     2401 aaaccagttt gcgagcgtct gggagatcag cagtctggag ctgcgcctga ctctggcgga
     2461 ggcgggcatg ggcgtgacct acgtttccga ccggctggtc ggcgacctgg aggggttcca
     2521 tcccatagat cacatggaaa tctcgaccat tccccgagtg gtgggcatct tccacaagaa
     2581 acacgttgcc ctttcggaag gcgccaagcg ctttctggcc atctgccagc ggcggtttgc
     2641 cggctgattc gagcgggtca gccgccgtcg tggggcacgt tgtcggcgcg ggccgccctg
     2701 cccagatagc cgccgtgatg gcgcaagccc cagtcttcct tggtgatgcc cttggcctcc
     2761 atgagcgcca gcgcaatggc gtcgctgatg gcgagcatga cggccgtgct ggcgctgggg
     2821 gtcagcccca ccgggcacgg ttcttcgatc tcgcccatgt ccagcaccag gtcggaaagg
     2881 tcgcgtagct cggagtcggg atgggaggtg atgccgatga ccatggccag gcccaggtgc
     2941 ctggccagct cgagtatttc cagcacctcg cggctcttgc cgctggtcga gaaggcgatc
     3001 atcacgtcgt cgggtcccac cagccccagg tccccgtgag cggcttcggc agggtggatg
     3061 aagtctgccg gcgtcgcgcc ggagcagagc gtggcggcaa acttgcgggc gatgtgaccc
     3121 gccttgccga tgcccgtggt gatcaccttg ccgcggcact cgagcagggc gcggacggcg
     3181 tgctcaaacg tctcgtcgat ccgaaccgcc gcaatggcct cggcctcggc ctgcagcacg
     3241 gcccgcatac gttcctgaat gttcattgcc gacacggtcc gcgattgccc ggcgcaatgg
     3301 tgtcacgcgt atccggcgtc ctcaaggtga cgccgtgcat tgacagcgtc tggcgcctct
     3361 tacagagtcc gggtctcttc gacgttcgag gcacgacgct tgccgtacat agcgggaccg
     3421 gaaggggcgc tgttacccct gccgcgccgg tttgccccgg ggcctggtgg gccgggggcg
     3481 gtgggcgtcg tggatgcccg ctttgccgac gccagctcgg cgcggctggt acgcgcggtg
     3541 gaacggctga atgaccgtct gggccggcgc aacggggagg cgggtctgaa aatggccctc
     3601 gagatctcct gcgcgatccc gtgtccggag caccctcagc tgggccagcc ggaggcttac
     3661 cagctcaacg tgactgacca gggcgcccgg ctcgtggcgg agaccgagtg gggggtcctg
     3721 cacgggctgg aaaccctgtt ccagctggcg acggcgccgg gcggcctcgg ctgcgtggct
     3781 gtcgacgatg cgcccagatt tccctggcgc ggtctgctcc tggacgtggc ccggcactat
     3841 ctcggcatgg acgcgctcct gcgtacgctc gacgccatgg ctcaggtgaa gctgaacgtc
     3901 ctgcatctgc acctgacgga cgaccaggcg ttccggtttc ccagcgagcg attccccgag
     3961 ctcgccagcc cacgacacta ttcccgacgc gagctcgccg agctcatcaa ccacgcggcc
     4021 gaccgcggca ttcgcgtggt gcccgaaatc gacgtccccg ggcacacgac ctgctggctc
     4081 gtggcccgcc ccgactgggg gaccggcgtg acgacgtcga ccaggcggtt cggtgtccac
     4141 gaggcctgcc tggatcccac cagcgaagcc gtctacgccg ctctggccga gctgctgggc
     4201 gaggtggccg aggtgtttcc ggatcgccat ctgcatctgg ggggcgatga ggtgcatccg
     4261 gcttggtggt cggcaagcga ccgcgttcag accttcatgt cggagcgcgg catgaccggg
     4321 ctcgacgaca tgcaggccca cttcaactcc cgggtggccc gcacggtgag tgggcttggc
     4381 aagcggccca tgggttggga cgaggtggtg cacgacgatc tgcccgacga cgtggtcgtt
     4441 cagagctggc ggggcgcgac cgcgcgggac cgggctctgg gtagcggcca cgactgcgtc
     4501 gtttcgtcgg gctactacct ggacctcttc tatccggccg acgtgcacta ccgcttcgat
     4561 ccgcaagccc cggagtccga gctcgtcggg ctcgaggatg cgctcctcga cgacccccgg
     4621 ttcgagcacg tggccgaggg catgcgctgg accgagcatt ggcgcgagcc tgccggcgca
     4681 gccggcgctg cccccggttc ggttctgggc gccgaggcgt gcctgtgggg cgagctggtc
     4741 accgaggagc tgctcgacgt gcgcctgtgg tcccggctgc cggccctggc cgagcgattc
     4801 tggtcgcccc gctcggtgcg cgatctgggg agcctttacc agcggctggg aagctgcttt
     4861 gagcagctcg ccgcgggctc gggggtggac gtccgggcgg cgagccgtgc cctcatggcg
     4921 cgggcgggag cggacgagga cgtcgtcgag ctgctcgagc tgctggaacc ggtcaagtgg
     4981 tacggacggc tcctgggcga ggaagcgctc gccgcccgca tcgcgggaac cgagatgccc
     5041 caggcccggc cctacgatgc ggacacgccg ctggacaggg ttgtcgatgc gctgtttccg
     5101 gagagcctgc ccgcgcgccg tgtggccagg ctgtgccaac gtcacttcga aggcgacacc
     5161 ggcgctgtgg cgggtatcct ggattgcgcg ctggcatggc aggaccttgg cccgcggccg
     5221 gactgcccgg ccgacgtcgc gcccgccctc gagctcctct gccgcgtggg cgaggcggtg
     5281 gtggacgtgc tcgaaggttg catggcgcct tccgctgcgc tgtcgctgct ggagacggct
     5341 gccaggcccc agggtgagta tctcatcgcc gtggtgccgc ccctgcggtc gtggctcgcg
     5401 gggctgggcg gatgatcgat ccgttgcagg tggcccgctc cggctggcga gtcggcaagg
     5461 aggtcgatcg cctgggtgcc ggacacatca acgacacgta cctggtgacc gacggcgtcg
     5521 accgctgggt cctgcagaga atcaacgacc aggtgtttac ggacccggcc ctcctgatgc
     5581 gcaacctggg ccgggtagtg gaacacgttt gcgctcgcgc gccgggattc gtgcccgggc
     5641 tgctgtcgtg ccgcgatggc tcgaacgtct tcgtggacga ggaaggcggc agctggcgcc
     5701 tgacggagta cgtgagcgga accgaaagcc accgtcgtct cgaaagcgtc gaccaggcaa
     5761 cgtcagcggc gcgtgcgttc gcccgttacc agaatcttct cgccgatctt ccggccccgc
     5821 cgctcgatga tccgataccc ggcttcatgc ggctcgacgg ctacctcgca gcgctcgatg
     5881 ccgccacagc acgcgcggca ggcgagcacg gtgcggagga ccttctcgga ttcgtggagg
     5941 ggcgacgaga tctggcccag gtgctgcagg atccgggcaa gacgggtggt tacattcatg
     6001 gcgactgcaa gatcaacaac ctgctctttc gtgagaacag ctcggaggtg gtctgcgtcg
     6061 tcgatctgga caccaccatg cacggccact gggcctggga cttcggcgat ctggcgcgct
     6121 caggcgcggt gacggaggcc ggggcgctgt ctctcgagct cttcggagcg ctggtcgagg
     6181 ggtttgccgg tgagaaggaa gccgcgctcg gcgcggacga gcttgtcctt gctccgcgtt
     6241 acgtctgctt catgctgggc gttcgcttcc tcacggatca tctggacggc gaccgctact
     6301 tcaagatcgc cgcccgcggc gacaacctcc gccgcgcccg ggcgcagttt cgcctgctcg
     6361 aggagatgga ggcgcgggag tcggagatgg ccgaggtcgc cgcgagtcgt cgctagctct
     6421 cgatgccggc gaggacgaga tgaacgagcg ccgcgatgcc gaggatcagc agggcagtga
     6481 gcaccacgcc gacggcgatg aactgcgcgg gccggcctct gctgaagtcg cgcgtccggt
     6541 tgcggcttga ctgcacgccg aaggcggcgg ccagagcgct ggccacggtc tgcgtcaggg
     6601 tcagggggcc gcgctccggc gggtcgggat cgtggtcgtc ctctgtgggg atgctcatgg
     6661 ggggagtata gcgccgggcg agcgggcggc agcgtcgtcg accggcgcgg tcttgccagg
     6721 gccggagcga aggtgccatg ctcgccggct ggcgggaatc accaagcggc tttgcgatga
     6781 ctcgatgtgc ggccctcatt cttgtcgcga tcctcctctg cggatgcgcg gtatcctcga
     6841 accggccgac ccagctcgtc tcgggcagcg cggcctaccc ggaagatgcc cgggcccagg
     6901 gaatcgaggg gctcgtggtg gtccgctacg acgtggcggt ggacggccga gtgatcaatg
     6961 cccgggtggt tcgcgccgag ccgccggacg ttttcgatga tgcggcgttg gcttccgtgc
     7021 gcagctggcg tttcaacccc gctcttgccg atggcgtgcc ccgggcgacc caggacatcg
     7081 agagcacggt gaccttcaag ctcgacggcg gggacgagta cgccgattac tgatggcgtt
     7141 cgggaccacc gcctgacccc gaagatcctt tcaggataac cgcgctcgtg gtgctcgggc
     7201 tgggcgccgc ccggtcgcta ccactcgttc cagtgggtga tgcc
//
LOCUS       DBNHKV010000057         2474 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1001652, whole genome shotgun sequence.
ACCESSION   DBNHKV010000057 DBNHKV010000000
VERSION     DBNHKV010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1001652"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="AC16PP_01655"
     CDS             complement(<1..484)
                     /locus_tag="AC16PP_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR6480357.1"
                     /translation="MMRKLLISALVALVLCNWAIASENLDPNLPEYQRVGGVSGNLSS
                     TGSDTLANLMTLWTESFKREYPNVNIQVQAAGSSTAPPALTEGTSNFGPMSRLMKDKE
                     IESFESRFGYKPMAIRVAIDALAVYVHKDNPIETMTISEVDAVFSATRRCGGPQDIGR
                     W"
     gene            complement(478..1806)
                     /gene="phoR"
                     /locus_tag="AC16PP_01660"
     CDS             complement(478..1806)
                     /gene="phoR"
                     /locus_tag="AC16PP_01660"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HIR6480358.1"
                     /translation="MKISRQEWILGTTLFVLAALGGWAFGNFGWAFLIALGIHAAVHR
                     RERLAFFRWAERPLRRPENRIESWESRVSRVFRTIRRERGRHRAALRQLRAIRTIADA
                     LPDAVVVIDRSGDIEMFNDAARDILQLHRRDRGSNLSALVRQPDFVALITGKLADDIV
                     EFASPFAGERRLEARRISLVDARSLVLVRDVSQLNRLLSMRQDFIANVSHELRTPLTV
                     IVGYLETLAGEQLDESTTRELIGKLGSPTKRMQALVNDLLLLTRLEASPSPSDDELHA
                     VDMGLIIEGIAIDARALSDGHHRISVRLDCDLRVLGIESELHSACANLLNNAVRYSPE
                     GGDIQVTWENIGPAARFSVNDHGLGIPPEHLSRLTERFYRVDLARSSVRGGTGLGLAI
                     VKHVLKRHGSELQVKSELGEGSTFYCDFPATRLADPLVSNQSGSSSLEKS"
     gene            complement(1814..>2474)
                     /locus_tag="AC16PP_01665"
     CDS             complement(1814..>2474)
                     /locus_tag="AC16PP_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6480359.1"
                     /translation="DEPEIRDMLRFALTRSGFQVWDTESAEDAMARLDGVLPGLIIVD
                     WMLPGMSGVDLARRVRNDEVTGHLPIIMLTARGEETDKLKSFDSGVDDYVTKPFSPKE
                     LVARVKALLRRSGSPQDGIIDTDGIQLDLNAHQLIIKGQPVKIGPTEFRLLELLLSNP
                     DRAFDRSQLLDRVWGRSVYVEERTVDVHVLRLRKVLKPYGLNRLIQTVRGVGYRFSPG
                     S"
BASE COUNT          449 a          737 c          818 g          470 t
ORIGIN      
        1 cccaccggcc gatgtcctga ggtccaccac atcggcgcgt ggcggagaaa acggcgtcta
       61 cctcgctgat ggtcatggtc tcgatgggat tgtccttgtg gacgtagacg gccagcgcgt
      121 caatggcgac ccggatggcc attggcttgt aaccaaagcg ggattcgaac gactcgatct
      181 ccttgtcctt catgagccgg ctcatcggcc cgaagttgga ggtgccttcg gtcagggcgg
      241 gaggagcggt agacgacccg gctgcctgta cctggatgtt gacgttgggg tactcgcgtt
      301 tgaaggattc cgtccaaagg gtcatcaggt ttgcgagggt gtccgagccg gtgctcgaca
      361 ggttgcccga gacgccacct acccgctggt attccggcag gttcggatcg agattttcgc
      421 tcgcgatggc ccagttgcag agcaccaggg cgaccagagc cgagatcagt agctttctca
      481 tgatttctcc aaggaggatg atccggattg attggaaacg agcgggtcgg ccaatcgagt
      541 ggcgggaaag tcgcagtaga acgtgcttcc ttcgccgagt tcgctcttta cctgcagctc
      601 gctgccgtgg cgtttgagga cgtgcttgac gatggctagc cccaagccgg tgccgccgcg
      661 cacacttgat cgggcgagat cgacccgata gaaacgctcg gtgagccggg acaggtgctc
      721 tggaggaatg ccgagcccgt gatcgttgac cgagaacctc gcggcgggtc cgatgttctc
      781 ccaggtgacc tggatgtcgc cgccttcggg gctgtagcga acggcgttgt ttaggaggtt
      841 cgcgcaggca ctgtgcagct cgctttcgat gcccaggacg cgcaggtcgc agtccagccg
      901 gacggaaatc cggtgatgcc catcgctcag tgctcgcgcg tcgatcgcaa tcccttcgat
      961 gatgaggccc atgtccacgg cgtgaagctc gtcgtccgac ggggacgggc tagcctccag
     1021 ccgggtgagc agcaggagat cgttgaccag ggcctgcatg cgtttcgtcg gggagccgag
     1081 cttgccgatc agctcccggg tggttgattc gtccaactgc tcgccggcca gcgtctcgag
     1141 gtagccgacg atcaccgtca gcggtgttcg caactcgtgg gaaacgttcg cgatgaagtc
     1201 ctggcgcatg ctcagcagcc ggttgagctg cgagacgtct ctgaccagca ctagggagcg
     1261 cgcgtccacc agcgatatcc ggcgagcctc caaacggcgc tcgccggcga acggagacgc
     1321 gaactccacg atatcgtcgg caagcttccc ggtgatgagg gctacgaagt cgggttgccg
     1381 cacgagggcg gagaggtttg agccgcggtc gcgtcggtgc agctgcagga tgtccctggc
     1441 ggcgtcgttg aacatctcga tgtcgccgga tcggtcgatc acgaccacgg cgtccggcag
     1501 ggcatcggcg atggtgcgaa tggctctcag ctggcgcaag gcggctcgat gacgaccccg
     1561 ctcccgtcga atcgtccgaa agacccggga gactctcgac tcccatgact cgattcggtt
     1621 ctcggggcgt cgcaggggcc gctcggccca tcgaaagaag gcaaggcgct cgcggcggtg
     1681 gaccgcggca tgaatcccga gcgcgatcag gaaggcccag ccgaagttcc cgaacgccca
     1741 tccgccgagg gcggccagga cgaaaagcgt ggttccgagg atccattcct ggcgggagat
     1801 tttcatgggt tggtcacgaa cccggcgaga aacgatagcc cacaccgcga acggtctgga
     1861 tcagtcggtt caggccatac ggcttgagta ccttgcgcag cctgagcacg tgcacgtcca
     1921 cggttcgttc ctccacgtac acgctccgcc cccacaccct gtcgagcaac tgggagcgat
     1981 cgaaggctcg atccgggttg ctgagcaaaa gctccagcag ccggaactcg gtggggccga
     2041 ttttcaccgg ttgtcccttg atgatcagct gatgggcgtt cagatccagc tgaatgccgt
     2101 ccgtgtcgat gattccatcc tgcggagagc ctgatcgtct aaggagggct ttaacccggg
     2161 cgacgagctc tttcggcgag aacggcttgg taacgtagtc gtcgacgccg gagtcgaagc
     2221 ttttgagctt gtcggtttcc tcgcctcgtg cggtgagcat gatgatggga agatgtccgg
     2281 taacctcgtc gttgcgaacg cgcctagcga ggtcgacccc gctcatgccg ggcagcatcc
     2341 agtcaacgat gatcagcccg ggcagaacac catcgagccg ggccatcgcg tcctccgccg
     2401 actcggtgtc ccagacctga aagccggagc gcgtgagggc gaatcgaagc atgtcccgga
     2461 tttcaggttc gtcc
//
LOCUS       DBNHKV010000058         4918 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_576016, whole genome shotgun sequence.
ACCESSION   DBNHKV010000058 DBNHKV010000000
VERSION     DBNHKV010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_576016"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="AC16PP_01670"
     CDS             <1..774
                     /locus_tag="AC16PP_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480360.1"
                     /translation="LGLLARCDCPYHNVEHTIMVADAGLAILKGQLALEDPISPDDWL
                     HAVVAMLFHDVGYLRDLLPEDGPEGCLVEHGGRRVSPPADATDAWMMPYHVTRGDLFV
                     RQHYADHPLIDVDRVAAYIEMTRFPVPDRPPYQQLETLGALVRAADLIGQMGDPNYLA
                     KLSSLFVEFKENGEADRLGFASVAELRADSQAFFSQQISPYLPKALKLLRATEEGRAW
                     VVSLYENIERRAQAGGSAAQPDPSKQENSRAGRIHAGNG"
     gene            854..2128
                     /locus_tag="AC16PP_01675"
     CDS             854..2128
                     /locus_tag="AC16PP_01675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6480361.1"
                     /translation="MSELATYEFGQEAPSGRRPLRIALLGYRSNPFSGGQGVYLKFLS
                     RALVDAGHEVDVISGEPYPELDGRVGLIKLPGLNLFDVPNHVTALRPKHLLSATDFFE
                     WLSMLSGGFPEPYTFGRRLTRYFSRIDDRYDVVHDNQSLCYGLLNVARRTPVICTIHH
                     PITWDRDIAIANAATRGERLLIRRWHSFLAMQTRVAKRLPNIVTVSERSKRDICAAFE
                     IPQGRVTVVRNGIDTEAFRPDPTVTRKPWQLVTTASADQPLKGAQHLLPAFAQLCRRF
                     PELRLVFIGAPRPGGKTDRLVDALGVRHRMDFIHGISTADIRRLYAESTVAVVPSEYE
                     GFGLPAGEAMACGTPLVSTDGGALPEVVGQAGRIVPSRDPDALAEAIAGLLVDPKERE
                     ALSRAGRARMVEHFAWAEAAERLTALYEQVLA"
     gene            2125..2886
                     /locus_tag="AC16PP_01680"
     CDS             2125..2886
                     /locus_tag="AC16PP_01680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6480362.1"
                     /translation="MSLETVDFARLKLAPGDNVLDLGCGEGRHAITAYLREAVTVVGM
                     DLSLGDLTTARQRYGEFRDGDDASRRVDFILATGHNLPFADATFDKVICAEVLEHIHD
                     YENVLGEIHRVLKPGGTLAVSVPRFGPEWLCWRLSDDYHQVEGGHVRIFRSRELRRSI
                     EACAPEPAGARPVAGKTQLSFQSRHFAHGLHSPYWWLRCLFWRRGESVWPVRLYHRFL
                     VWDLMRKPLLTRVLERALNPLIGKSVVMYFHRGAV"
     gene            2883..3950
                     /locus_tag="AC16PP_01685"
     CDS             2883..3950
                     /locus_tag="AC16PP_01685"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HIR6480363.1"
                     /translation="MTRPESLHRTARYIASVQQNDGAIPWFAGGITDPWDHVEAAMGL
                     SIGGHHAQAAAAYRWLARMQRADGSWLAAYSGARVADGTRAETNFVAYVATGTWHHYL
                     ISEDGKFLRSMWPTVKRALDFVTGLQAPTGEIYWAQDTRTGINKDALVTGCSSIYKSL
                     ECGIFMAAELNEDPGAWPACRARLGDAIILRPDRFDRTWESKSRYSMDWFYPVLAGLF
                     SQADARERLDERWETFVAPSLGCRCVSDQAWVTVAESCELAIACVASGEPDKAEELCD
                     DLARFQAEDGSWWTGYAFEDDALWPDERPTWTAGAVLLAADAMHGLTPASALFTDNAL
                     PDAPEHSERAGRFQRLEQARG"
     gene            complement(3882..4496)
                     /locus_tag="AC16PP_01690"
     CDS             complement(3882..4496)
                     /locus_tag="AC16PP_01690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6480364.1"
                     /translation="MKLPLDPDEIKGFLHPEEGARLYALASVASGLGPCLEVGSYCGK
                     STLYLGTACRDQGQVLYAVDHHRGSEEHQPGEAYHDPELFDAEIGSMESLMAFRRTMY
                     RAGLEGCVIPVVAASALAGRHWATPLALVFIDGGHSVEAAQTDYRTWAPHVVPGGFLA
                     IHDLFPDPLEGGQAPIDVYRLASASGLFEPLETTRTLGVLRRVG"
     gene            4619..4750
                     /locus_tag="AC16PP_01695"
     CDS             4619..4750
                     /locus_tag="AC16PP_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480365.1"
                     /translation="MHDENEREPVEELFAAEAPFAFPDQLYNEDDAEDNLSYVRGYN"
BASE COUNT          865 a         1683 c         1547 g          823 t
ORIGIN      
        1 ctgggactgc tcgcacgctg cgactgcccc taccacaacg tcgagcacac catcatggtg
       61 gcggacgccg ggctggcgat cctgaaaggg cagctcgccc tggaagatcc catctcgcca
      121 gacgactggc ttcatgcggt ggtcgccatg ctctttcacg acgtcggcta tctgcgggac
      181 ctcctgcccg aggacgggcc ggagggctgc ctggtggaac atggcggcag gcgggtatcg
      241 ccccccgcgg acgccacaga tgcctggatg atgccgtacc acgtcacccg cggtgatctc
      301 ttcgttcgcc aacactatgc agaccatccg ctgatcgacg tcgatcgcgt ggccgcctac
      361 atcgaaatga cgcgcttccc ggttcccgac cggccaccct accagcagct ggaaaccctg
      421 ggcgcgctcg tgcgggcagc cgatctgatc ggccagatgg gcgacccgaa ctatctggca
      481 aaactgtcca gcctgttcgt ggagttcaag gaaaacggcg aggctgaccg gctcgggttc
      541 gcgagcgtgg ccgagcttcg cgcggattcc caggcttttt tcagccagca gatttcaccc
      601 tacctgccta aagcgctgaa gctgcttcgc gcaaccgaag agggccgggc ctgggtcgtc
      661 agcctgtacg agaacatcga gcggcgggcc caggcggggg gctctgcggc ccagcctgac
      721 ccatcaaagc aggagaattc ccgcgctggc cgtatccacg cgggcaacgg ctgaccctac
      781 cctcatccgc cgtccaacgc ctggcactcc ttcgcgccgc tgattagact tgtccgcttt
      841 gctcggggca ggcatgtcgg agctcgcgac ctacgagttc ggccaggaag cgccttctgg
      901 tcggcggcca ctgcgaatcg cgctgctggg gtatcgcagc aacccgttca gtggcgggca
      961 gggcgtgtat ctgaagttcc tctcccgcgc cttggtggat gccgggcacg aggtggacgt
     1021 gatatccggc gagccctacc cggaactgga cgggcgcgtc gggctgatca agctgccagg
     1081 cctgaacctg ttcgacgtgc ccaatcacgt gaccgcgctg cgcccgaagc atctcctttc
     1141 ggcaaccgac ttcttcgaat ggttgtccat gctgagcggc ggattcccgg agccgtatac
     1201 cttcgggcga cgcctgacga gatacttctc ccgaatcgac gatcgctacg acgtcgtaca
     1261 cgacaaccag agcctgtgct acggcctgct gaacgtcgcg cgccgaacgc cggtgatctg
     1321 caccattcac catcccatca cctgggaccg cgatatcgcc atcgccaacg ccgcaacccg
     1381 gggcgagcgg ctgctgatcc gacgctggca cagctttctt gccatgcaga cccgcgtggc
     1441 aaagcgtctg cccaacatcg tcaccgtcag cgagcggtcc aagcgcgaca tctgcgcggc
     1501 tttcgagatc ccccagggcc gggtgaccgt cgtcaggaac ggcatcgaca ccgaggcgtt
     1561 ccgcccggat ccgaccgtga cgcgcaaacc ctggcaactc gtcaccacgg cgagtgccga
     1621 tcagccactc aagggcgccc agcacctgct acccgcgttc gcccagctgt gccggcgctt
     1681 tcccgagctg cgcctagtct tcataggcgc cccgcgtccc gggggcaaaa cggatcgcct
     1741 ggtagacgcg ctcggcgtgc gccatcgcat ggatttcatt cacgggatca gcacggcaga
     1801 catccggagg ctctatgccg aatccaccgt ggccgtcgtc ccgtcggaat atgagggctt
     1861 cggactaccg gccggcgagg cgatggcctg cggtacgcca ctggtgagca cggacggcgg
     1921 cgcgcttcca gaggttgtcg gtcaggcggg ccggatcgtg ccgagccgcg acccggacgc
     1981 gctggcagag gccatcgctg ggcttctggt cgacccgaag gagcgcgaag cgctcagccg
     2041 cgccggccgg gcccgcatgg tcgaacactt tgcctgggca gaagccgcag agcggctcac
     2101 cgctctctac gagcaggtgc tcgcatgagt ctggagaccg tcgatttcgc gcgcctgaaa
     2161 ctcgcccccg gggacaacgt cctggatctt ggctgcggag agggccgcca tgccatcacc
     2221 gcctacctca gggaggccgt cactgtcgtc ggcatggatc tgtcgctcgg cgatctgaca
     2281 accgcgcggc aacgctacgg cgagttccgc gatggcgacg acgcctctcg gcgggtggac
     2341 ttcatcctcg ccaccgggca caacctgccg ttcgccgacg ccaccttcga caaggtgatc
     2401 tgcgcggaag ttctcgagca catacacgac tacgagaacg ttctcggtga gatccatcga
     2461 gtgctcaaac ccggcggaac gcttgcggtc agcgtgcccc gattcggtcc ggagtggctc
     2521 tgctggcgac tttccgacga ctaccaccag gtggagggcg gccacgttcg catcttcagg
     2581 tcccgggagc tgagaaggtc catcgaggcg tgcgccccag aaccggccgg agcgcggccg
     2641 gtggccggaa agacgcaact gtcgttccag agccggcatt tcgcccacgg cctgcattcc
     2701 ccgtactggt ggctgcgctg cctgttctgg cgccggggcg agtcggtctg gcccgtccgc
     2761 ctctatcacc gcttcctggt ctgggatctc atgcgcaaac ccctgctcac ccgtgtcctg
     2821 gaacgcgccc tgaacccgct gatcggcaaa agcgtggtca tgtactttca ccggggtgcc
     2881 gtatgacgcg gccggaatct ctgcaccgca ccgcccgata catcgcctcc gtccagcaaa
     2941 acgacggcgc gatcccctgg tttgcgggag ggatcacgga tccctgggac catgtggagg
     3001 ccgccatggg gctgagcatc ggcgggcatc acgctcaggc cgcggccgct taccgctggc
     3061 ttgcccggat gcagcgggcc gatggttcct ggctggctgc ctacagcggt gcgcgcgtgg
     3121 cggacggcac ccgagcggag accaatttcg tcgcctacgt ggcaacaggc acctggcatc
     3181 attatctgat ctccgaggac ggcaagttcc tgcgctccat gtggccgaca gtgaagcgcg
     3241 cgcttgattt cgtcaccggc ctgcaggctc ccacgggcga gatctactgg gcccaggata
     3301 cccgcacggg catcaacaag gacgccctgg tcaccggttg cagctccatc tacaagagcc
     3361 tcgagtgcgg catcttcatg gccgcggagc tcaatgagga cccgggcgcc tggcccgcat
     3421 gccgggcgcg actcggcgat gccatcatcc tgcgccccga tcgcttcgac cgcacgtggg
     3481 aaagcaagtc ccgctactcc atggactggt tctatccggt gctggcgggg ctcttttcac
     3541 aagccgacgc acgcgaacgg ctggacgagc gatgggaaac cttcgtcgcc ccttcgctgg
     3601 gctgccgctg cgtctccgac caggcctggg tcacggttgc cgagtcctgc gagcttgcca
     3661 tcgcctgcgt cgcttccggc gagccggaca aagccgagga gctctgcgac gacctggcga
     3721 ggttccaggc cgaggacggt tcttggtgga cgggatacgc gttcgaggac gacgccctgt
     3781 ggcccgacga gcggcccacc tggaccgccg gggccgtgct gctggccgcc gacgccatgc
     3841 acgggctgac cccggcatcg gccctgttca cggacaatgc gctacccgac gcgccggagc
     3901 actccgagcg tgcgggtcgt ttccagaggc tcgaacaggc cagaggctga cgccaggcgg
     3961 tagacgtcta taggcgcctg gccgccttcg agaggatccg gaaacagatc gtggatggcc
     4021 aggaaaccgc cgggcacgac gtggggagcc caggtccggt aatcggtctg agcagcttcg
     4081 acgctgtgcc caccgtcaat gaacacgagc gcaagcggcg tcgcccagtg gcggcccgcc
     4141 agcgcagacg ccgcaacaac cgggatcacg catccttcca gacccgcgcg gtacatggtt
     4201 ctgcgaaacg ccatcagcga ttccatgctg ccgatctcgg catcgaagag ttccggatca
     4261 tgatacgcct cacccggctg gtgctcctcc gatccccgat ggtgatcgac cgcgtagagc
     4321 acctggccct ggtcccggca ggccgtaccg agataaagcg tggacttgcc gcagtagctg
     4381 cccacctcga ggcagggccc taggcccgac gcaacgctgg cgagcgcgta gagccgtgcg
     4441 ccttcctccg ggtgaaggaa ccctttgatc tcgtcgggat cgagtggaag cttcatgtcg
     4501 aaaaaccgag cttttcgtcg caattcagcg caaatccgac ggcaacctcg cagaacggga
     4561 ttgcaatttc aacatctgac actagactcc cctcacacga ccctgccggt tcaacaagat
     4621 gcacgacgaa aacgagcgag agcccgtcga ggagctgttc gcggccgagg caccctttgc
     4681 cttcccggac cagctctaca acgaggacga cgcagaggac aatctcagct acgtccgcgg
     4741 ctacaactga ttcagccctc gcgccagctc agatcagctc cagcaggtag aacaccaccg
     4801 cgaacgccac cggcaaagcg atggcgacgt atgcgagcag gttgagcagc ccgttgcgct
     4861 cggcgtcctc ggcgagcttc agcctcgggc cgatgccaat ccatagcgcg ccgccggc
//
LOCUS       DBNHKV010000059        11100 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1354916, whole genome shotgun sequence.
ACCESSION   DBNHKV010000059 DBNHKV010000000
VERSION     DBNHKV010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11100)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11100)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11100
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1354916"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="AC16PP_01700"
     CDS             complement(<1..745)
                     /locus_tag="AC16PP_01700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6480366.1"
                     /translation="MKFGIFYELQLPKPWEADSEYRLFNNALDQIELADRLGFDYAWE
                     VEHHFLEEYSHSSAPEVFLAAASQRTRNIRLGHGIVQLPTNHPARVAERVSTLDLISH
                     GRVELGLGEGSSVTELHPFGRRFREKRAVWEDAVRATLPMFWEEGWEYHGEFFDFPLR
                     NVIPKPRQQPHPPLWVACSQMDTIRMAGRRGMGALGFSFVSPESAQAWCHAYYNEFVK
                     RQDRLCDYQPNANLAVVSPFMCCETDEDAQ"
     gene            complement(776..1066)
                     /locus_tag="AC16PP_01705"
                     /pseudo
     CDS             complement(776..1066)
                     /locus_tag="AC16PP_01705"
                     /inference="COORDINATES: protein motif:HMM:NF041106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1204..1338)
                     /locus_tag="AC16PP_01710"
     CDS             complement(1204..1338)
                     /locus_tag="AC16PP_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480367.1"
                     /translation="MTTGMTVMIVMTTLAVTVGVVALRVSWRIVNRLSEERPQNEEER
                     "
     gene            1464..3251
                     /locus_tag="AC16PP_01715"
     CDS             1464..3251
                     /locus_tag="AC16PP_01715"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6480368.1"
                     /translation="MYDRATTYGSDRVHEGPCHEHGPHGCPCCAATLGELLRRVGLDV
                     EAQAGRSVPAAPRAPVPTLFCNARVLTMDDAGSEFDTLLISDGRIQWVGNADADAVPE
                     VGDDTVRVDCNGRTILPGFVEPHMHLSAIAMLRRFENVGPFRYPTVSGALARLKEVAA
                     DTDPGAWIIGRQFDPSLQEGPGTLTASMLDEVSTTHPIFVYNASLHFGYCNSLALEIA
                     GITRDTPDPEGSPFGRLPDGTPDGVLKGGVSQAMVARHNPMLREHDLVEGCLDVFRHA
                     NSVGFTTICDQGTGAMQGAKELALYEALRESNRMTARFRYSLFNTLAADWDASDVSFG
                     TGDSWVRASAWKIVSDGSNQGLSGLQREPFMNADSHGIAYIEPEALNEAVKRRLGQGW
                     PVAVHANGDLAIDRVLDAFEAARAAGLDPARARCRIEHCSILHDEQIERIADLGLSPS
                     FLIGHVYWWGKAFRDEIFGLEKASLLDRTAACEAKGVRWTLHSDEPVTEMGPLRCIEN
                     AVTRRLWRTDDEVLAPHECVGVEQALRAMTRDAAWQCHSDHEIGTLEPGKLADLVVLE
                     EDPRSVPPSRLGAVRVLETWVGGERVYQA"
     gene            3294..4667
                     /locus_tag="AC16PP_01720"
     CDS             3294..4667
                     /locus_tag="AC16PP_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HIR6480369.1"
                     /translation="MTSTFPGVIAFAFMVCMLLAGTVLRAKVPLLRRALVPASLVGGI
                     LGFALVSAGLSFGYESSDFTVFTFHFFTLSFMSLCLTGGSRERGKNTSIIPGGMWMSV
                     VWVVSLVTQGLVGLGALVGYNVLTGGELSSFLGLLATHGFTQGPGQALAYASIWESEL
                     GVVHAINFGIIYASAGFIVAFAVGVPVARHAIKAGRNVNRVARIDDEFQAGILNADSD
                     VSAGRQITHSANIDTLAFHIAVLGVAYLVTDQYLSFMRPIADGWAIGDVNFGVLFSHN
                     LFFVHGLMVCVIMRSLLDRFGLGHYIDDETQRRITGTSVDFMVVATIMSIHFGLLSEF
                     IVPILVVCLAVSAVTVVLCFGLGRHLPNLSAERSLTSFGCCSGSTGSGLLLLRIVDPD
                     LSTGIAKELAFFNIAILVLSFHVLGFMAPILPSYDLTFICAVYAATLVVGLVAVRMVR
                     KRMMAAA"
     gene            4671..5903
                     /locus_tag="AC16PP_01725"
     CDS             4671..5903
                     /locus_tag="AC16PP_01725"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6480370.1"
                     /translation="MDRGLRWYLLSTGLFLVPGGIQMVLFPWLVAVYLQESPARVGIA
                     QMSLQLPMLLLILWGGLVGDRFDQRRLLVGLHLGMIGPPLAMAVLVQADLIVYEVLLG
                     WAVIGGSFGAFAQPARDALLNRVAGGDVQGVVTLAIGVQFGVQIIGFALGSTADSLGP
                     LWLFLVQALLMGAAAVATSKIPLLPMGSARERRPALREIGEGIVMAWRSEAIRPAVLL
                     TFAQGVFFAGAYLVILPLMVRDLYGGGAGGIAGAFAANMLGTCTVVFFLRRRGGIDRP
                     GRALGLWGVFGVCVLSLLHFELPLWGFYAVIYLWGTCGGVSMTMSRSIVQEASPPSHR
                     ARLMSVYSLGLMGGMPFGSAFLGWCVGVLGARNAVLVPVIGMAVVQLLIFTTSRLWLV
                     ERQRGSDLDEEPLMHAAD"
     gene            complement(5921..7552)
                     /locus_tag="AC16PP_01730"
     CDS             complement(5921..7552)
                     /locus_tag="AC16PP_01730"
                     /inference="COORDINATES: protein motif:HMM:NF004807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HIR6480371.1"
                     /translation="MKVAQAIAKALKAEGVEVLFAYPVNPIVEAAAAEDIRTVIVRQE
                     RVGLHMADAYSRLSSGDRIGVFAMQHGPGAENAFGGVAQAYSESVPILVLPAGYHRRI
                     ANYYPNFSSTLNMRNITKWAEPLTMGKAVPEVMRRAFFQLRTGRPGPVLVEIPVDVFR
                     EDVPDDWHHTPSFKTRSAPDPDAVATAAEVLAKAERPVIYAGQGVHYAKAWAELKALA
                     ETWNIPVTTSIEGKSAFPENHPLSLGSGGRANPRPVKAFLNEADVILGIGCSFALTGF
                     GVPMPAGRTIIHATLDPMDLNKDVPAQHGLIGDAALTLRALSQAMDGKDRSPAEARAG
                     VSGRIAAIREEWLGEWMPKLTNDEAPINPYRVLWELNQMVDKDNTVITHDAGSPRDQT
                     TPFWECTTPLSYIGWGKTTQLGYGLGLAMGAKLACPEKLCINVWGDAAIGFTGMDFET
                     AVRERIPILSILFNNFSMAIEIPIMQISQAKYGATDISGNYADMAKAFGGYGERITDP
                     GDIRAALERGIEATRAGSPALLEFITDKEITVSNE"
     gene            7646..8281
                     /locus_tag="AC16PP_01735"
     CDS             7646..8281
                     /locus_tag="AC16PP_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HIR6480372.1"
                     /translation="MADYPSQRGIVLWAAPFLAVLLWSAVNPKDYEVWVLEVSPAVAG
                     ALIIFLTRRRFPLTRLTYGLILVHALILIVGGHYTYAEVPLFDLLREPLGFTRNNYDK
                     LGHLAQGFVPAIVAREVLLRLSPLERGGWLNLFVVSLCLGISAFYELIEWWVALLSEQ
                     AAESFLGTQGYVWDTQSDMGWALLGAVLALVLLARYHDRQLLEQGLAGNHD"
     gene            8274..9605
                     /locus_tag="AC16PP_01740"
     CDS             8274..9605
                     /locus_tag="AC16PP_01740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spinster family MFS transporter"
                     /protein_id="HIR6480373.1"
                     /translation="MTDQVEEQPAGAPAAQGFSGKTPWRSWYALGVLFVVYVFNFIDR
                     SILGILNQAIKEDLALSDTQMGFLGGIAFAVFYTFLGIPIARLADRTVRRNLLAVCLA
                     IWSVMTALCGLASSFAHLLLARIGVAVGEAGGSPPSHSMISDMFPESSRATALSIYAL
                     GIPVGTMIGNLAGGWINEAFDWRTAFIVVGLPGVALAFLVVLTVREPVRGAADNMTAS
                     SEAAPPVMTVFRYLWSLKSFRYLSLAGALHAFVGYGVGYWFPAFFIRSHGLGTGEIGT
                     WLFYLGFAGMAGTFLGGYMGDRMARRDVRWYVWLPGIATLLSVPFSMVVYLYDDYRVA
                     FLVAIVPTLLGAYYLGPTFALTQGLVGLRMRALASSILLFILNLIGLGLGPQVTGILS
                     DGFNAFGDLGTDSLRYALICVLLLNVVATVYYWMAGRDLERDLARRGELDG"
     gene            complement(9639..9938)
                     /locus_tag="AC16PP_01745"
     CDS             complement(9639..9938)
                     /locus_tag="AC16PP_01745"
                     /inference="COORDINATES: protein motif:HMM:NF018717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR6480374.1"
                     /translation="MAAFFVAQYTVNNPALYAEYQQGAGPTLATYGAELVSFDVGAET
                     VEGNPPGPQTVIIKFESTDAAKAWYNSAEYQAVVGKRLEATDGFAVIAQSMNAGG"
     gene            complement(10034..11017)
                     /locus_tag="AC16PP_01750"
     CDS             complement(10034..11017)
                     /locus_tag="AC16PP_01750"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR6480375.1"
                     /translation="MATIAILGLGNFGTALARVWLLGGHDVRGWTVEEDVFDAIRSKG
                     SNSKYLDGIDLTGLQVSMELGESVPGAELVVLALPSHVVLHVADDVIPLLTPDQVVLD
                     LAKGIAGEELVSDAIQAKLAAAGKTNPLAVITGPTIAPELASGVLTTALVASEDESLA
                     SRLAEQLSTSTFSLKAASDPRGAELWGGFKNVIALGCGIADGLDEGSGDNLKAAIFTA
                     GFAEGNRLLTALGAAPETAFTPAGIGDLFVTASSPHGRNRSLGQKLGAGQTLEEAMAG
                     SVMVAEGVRATRMFAELAAARSVTTPFIAALTDLLDGNCTAPDCVRRLMAI"
BASE COUNT         1595 a         3589 c         3704 g         2212 t
ORIGIN      
        1 cctgggcgtc ctcgtcggtt tcgcagcaca tgaaaggaga gaccacggcc aggttggcgt
       61 tgggctggta gtcgcagagc cggtcctgac gtttgacgaa ctcgttgtag taggcgtggc
      121 accaggcctg ggcggactcc gggctcacga acgaaaagcc cagcgcgccc atgcctcggc
      181 ggccggccat gcggatggtg tccatctggg agcaggcgac ccacagcggc gggtggggct
      241 gctgtcgggg cttgggaatc acgttgcgca ggggaaagtc gaagaactcg ccgtgatatt
      301 cccagccctc ctcccagaac atgggcagag tggcccttac cgcgtcctcc cagaccgccc
      361 gcttttcgcg gaaacgacgg ccgaaggggt gcagctccgt gacgctggag ccttcgccca
      421 gcccgagctc cacgcggccg tggctgatca ggtccagggt cgagacccgc tcggccaccc
      481 tggcggggtg attggtgggc agctgcacga tgccgtggcc caggcggatg ttgcgcgtgc
      541 gctggctggc cgcggccaga aagacctctg gtgcggagga gtgcgagtac tcctcgagga
      601 agtggtgctc cacctcccac gcgtagtcga agcccagccg gtcggcgagc tcgatctgat
      661 ccagcgcgtt gttgaacagc cggtactcgc tgtccgcttc ccagggtttt ggcagctgca
      721 gctcgtagaa gatgccgaat ttcatcctcg cctccctctc cggccgctgc cggtcctacc
      781 cgccgaacac gccgccgtag gcctcctcgt tgaagccgac cagcagcgtc ttgcccactt
      841 tcagcgtagg ggcacgcaga ttgccggtcg gtccgagcat ggccgccacc gcgtcgtcgg
      901 tggccgcttt accaccgccg aactcgctga ttttcttacc cttcgcgacg atcattttcg
      961 acgctttgcc gagcagctcg cgggcgtccg gttccgtcag cttctggctg gccggcgtgc
     1021 ggtccttgat ctctattttg cttgcctcca agaacttgga tgctctggta cagctggttc
     1081 agccgttgcg gtagtaatac cagtcgacgc gctttgccac ggtcgtccct ccgttgattc
     1141 agactcgttg cggtatgggg gatcgcgccg acgggcccct gatggccgtc gctgggatag
     1201 cggtcagcgc tcctcctcgt tctgcggccg ctcttccgac agccggttca cgatacgcca
     1261 cgacacgcgc agagcgacga cgccgacggt caccgccagc gtggtcatga cgatcatcac
     1321 cgtcatcccg gtagtcatgg tgcttcctcc cgataccgtg gttgctacgg tgcctcattg
     1381 cgcgctccgg gtccagtgtt cccaatgtgg gctccatgca aatagcgtaa tctatgcgct
     1441 tgtggcctcg gggggagatg cgcgtgtacg acagggcaac tacctacgga tcagaccgcg
     1501 ttcacgaggg tccctgccac gagcacggcc cgcacggctg tccctgctgc gcggcgactc
     1561 tgggcgagct cctgcgccgg gtggggctgg acgtcgaggc ccaggcgggg cggtccgtgc
     1621 cggccgcacc gagggcgccc gtgcccacgc tgttctgcaa cgccagggtt ctgaccatgg
     1681 acgatgcggg ctcggagttc gacacgctgc tgatctccga cgggcgcatt caatgggtcg
     1741 gcaatgccga tgccgatgcc gtgcccgagg tcggtgacga caccgtgcgc gtggactgca
     1801 acggcagaac catcctgccg gggttcgtcg aaccccacat gcacctctcc gccatcgcca
     1861 tgctcagacg gttcgagaac gtggggccct tccgctaccc gaccgtgagc ggagcgctgg
     1921 ccaggctcaa ggaagtcgca gccgataccg accctggcgc gtggatcatc ggccgacagt
     1981 tcgacccctc gctgcaggag gggcctggca ccctgaccgc ctccatgctg gacgaagtca
     2041 gcacaacgca cccgatcttc gtctacaacg cgtcgcttca cttcggctac tgcaactccc
     2101 tcgcgctgga gatcgccggg atcacccggg atacgccgga cccggaaggt tcgcccttcg
     2161 gacgactgcc cgacggcacc ccggacggcg tcctcaaggg cggcgtctcc caggccatgg
     2221 tggcgcggca caaccccatg ctgcgcgagc acgatctggt cgaggggtgc ctggacgtgt
     2281 tccgccatgc caacagcgtc ggcttcacca ccatctgcga tcagggcacc ggcgcgatgc
     2341 agggcgccaa ggagctcgct ctttacgaag cgctgcgcga aagcaaccgg atgacggcgc
     2401 gctttcgtta ctccctgttc aatacccttg ccgccgactg ggacgcctcc gacgtctcgt
     2461 tcggcacggg agacagctgg gttcgcgcca gcgcctggaa gatcgtcagc gacggttcca
     2521 atcaggggtt gagcgggctg cagcgggagc cgttcatgaa cgccgactcc cacggcatcg
     2581 cctacatcga acccgaggcg ctcaacgagg ccgtgaaacg aagactcggg cagggctggc
     2641 cggttgccgt gcatgccaat ggcgatcttg ccatcgacag ggtgctggat gccttcgagg
     2701 ccgcccgggc cgcggggctc gatccggcgc gagcgcgctg ccgcatagag cactgcagca
     2761 tcctccacga cgagcagatc gagcggatcg ccgacctcgg gctctcgccc agctttctga
     2821 tcggccacgt ctactggtgg ggcaaggcct ttcgagacga gatattcggg ctggagaaag
     2881 ccagtctcct ggaccggacc gcggcctgcg aggccaaggg cgtgcgctgg accctgcact
     2941 ccgacgagcc cgtcaccgag atgggtccgc tgcgctgcat cgagaacgcc gtgacccgcc
     3001 ggctgtggcg gaccgacgac gaggtgctcg ccccccatga atgcgtgggc gtggagcagg
     3061 cgctgcgggc gatgacccgg gatgcggcct ggcagtgtca ctcggatcac gagatcggca
     3121 ccctggagcc gggcaagctg gcggatctcg tggtgctgga agaggatccc cgcagcgttc
     3181 cgccgtctcg cctcggcgcc gttcgggtgc tcgagacctg ggttggcggc gaacgggtct
     3241 atcaggcctg acgcgcagcc tcgggcgacg tttcacaaca gaagggtttc aacatgactt
     3301 cgacgtttcc gggggtgatc gcctttgcct tcatggtgtg catgctgctg gccggaaccg
     3361 tgctccgcgc gaaggtgccg cttctgcgca gagcgctcgt gccggccagc ctggtagggg
     3421 gcatcctcgg cttcgctctc gtctcggcgg gactttcctt cggctacgag agcagtgact
     3481 tcacggtgtt taccttccat ttcttcaccc tcagcttcat gtcgctctgc ctgacgggcg
     3541 gcagcagaga gcgcggcaag aacacctcca tcatccctgg cggcatgtgg atgtcggtgg
     3601 tctgggtggt cagcctggtc acgcagggtc tcgttggcct cggcgcgctg gttggctaca
     3661 acgtgctgac gggcggcgag ctatcgagct tcctcggcct gctggcaacc cacggcttca
     3721 cccaggggcc tggtcaggcg ctcgcttacg cctcgatctg ggagtcggag ctgggcgtgg
     3781 tccatgccat caacttcggc atcatctacg catcggccgg cttcatcgtc gctttcgccg
     3841 tgggcgtccc cgtggcgcgc cacgccatca aggcaggacg caacgtgaat cgggtcgctc
     3901 ggatagacga cgagtttcag gcaggcattc tgaacgccga ctccgatgtc tccgcaggac
     3961 gacagatcac ccactccgcc aacatcgata ccctggcgtt ccacatcgcc gttctcggcg
     4021 tggcctatct cgtgaccgac cagtacttga gcttcatgcg gccgattgcc gacgggtggg
     4081 ccatcgggga tgtgaacttc ggtgttctct tcagccacaa ccttttcttc gtccacggcc
     4141 tgatggtctg cgtgatcatg cgatcgctgc tggaccgttt cggcctgggc cactacatcg
     4201 atgacgagac ccagcggcgg ataaccggca cgtcggtgga cttcatggtc gtcgccacca
     4261 tcatgagcat tcattttggg ctgctgtccg agttcatcgt gccgatcctc gtggtctgcc
     4321 ttgccgtgtc tgccgtaacc gtggtgttgt gctttggtct tggcaggcac ctgcccaacc
     4381 tttcggctga acgctcgctg acctcgttcg gctgttgctc cggatccacg ggaagtgggc
     4441 tgctgctgct cagaatcgtc gatccggacc tgagcacggg catcgccaag gagctcgcgt
     4501 tcttcaacat cgccatcttg gtgctgagct ttcatgtgct cggcttcatg gcgccgattc
     4561 tgccgagcta cgatctgacc ttcatctgtg ccgtctacgc ggccaccctg gtggtgggcc
     4621 tcgtcgccgt tcgcatggtc aggaaaagaa tgatggcggc tgcctgaccc atggatcgcg
     4681 gcctgcggtg gtatctgctc agcaccgggt tgtttctcgt gcccggtggc attcagatgg
     4741 tcctgttccc gtggctcgtg gccgtgtacc tgcaggaatc gccggcccgg gtcggcatcg
     4801 cccagatgtc gctgcagctg ccgatgctgt tgctgattct gtggggcggg ctcgttggcg
     4861 accgcttcga ccagcggcgc cttctggtcg gcctgcacct gggcatgatc gggccccctc
     4921 tggcgatggc ggtgctggtt caggccgatc tcatcgtcta cgaggtgctg ctcggctggg
     4981 ccgtgatcgg cggttcgttc ggagcgtttg cgcagcccgc gcgcgatgct ctgctcaacc
     5041 gcgtggcggg cggcgacgtt cagggtgtgg tgacgctggc catcggggtt cagttcggcg
     5101 tgcagatcat cgggttcgcc cttggctcga cggccgattc gctgggcccg ctctggctct
     5161 tcctcgtcca ggcgctgctg atgggcgcgg cggcggtggc aacctcgaaa ataccgctcc
     5221 tgcccatggg gagtgctcgg gagcggcgcc cggcgctgcg cgagatcggg gagggcatcg
     5281 tcatggcctg gcgctcggag gcgatccggc cggccgttct gctcaccttc gcccagggcg
     5341 tcttcttcgc gggcgcctac ctcgtgattc tcccgctgat ggtccgcgat ctgtacggag
     5401 gcggcgccgg aggcatcgcc ggggcgtttg cggcgaacat gctcggcacg tgcacggtgg
     5461 tgttcttcct gcgacggcgc ggtggcatcg atcgtcccgg acgcgcgctg ggcctgtggg
     5521 gcgtcttcgg cgtctgcgtg ctttcgttgc tgcacttcga gctgccgctt tggggtttct
     5581 atgccgtcat ctatctgtgg ggcacctgcg gcggggtcag catgaccatg tcccggtcca
     5641 tcgttcagga agcgtctcct ccaagtcacc gcgcccggct catgtcggtc tattccctcg
     5701 ggctcatggg cggcatgccg ttcggatcgg ctttcctcgg ctggtgcgtg ggtgtgctcg
     5761 gcgcccggaa cgccgtgctc gtgcccgtca tcgggatggc cgtggtgcag ctgctgatct
     5821 tcaccacgtc gcggctttgg ctggtcgagc gccaacgcgg cagcgacctc gacgaagagc
     5881 cgctcatgca cgccgcggat tgacccgggc tgcgacgcgc ctactcgttg gagacggtga
     5941 tttccttgtc tgtgatgaac tcgagcagcg ccgggctgcc tgctcgggtc gcctcgatgc
     6001 cgcgttccag ggcagccctg atgtccccgg gatcggtaat ccgctcgccg tagccgccga
     6061 aggccttggc catgtccgcg tagttgccgc tgatgtcggt ggcgccgtac ttcgcctggc
     6121 tgatctgcat gatgggaatc tcgatggcca tggagaagtt gttgaacagt atggagagta
     6181 tgggaatccg ttcgcgcacc gcggtttcga agtccatgcc ggtgaagccg atggccgcgt
     6241 cgccccagac gttgatgcac agcttttcgg ggcacgcgag cttggctccc atagccaggc
     6301 caaggccgta gccgagctgg gtggtcttgc cccatccgat atagctcagc ggggtcgtac
     6361 attcccagaa gggcgtggtc tggtctctgg ggctgcccgc gtcgtgggtg atcaccgtgt
     6421 tgtctttgtc caccatctgg ttgagctccc acagcacccg gtaggggttg atgggggcct
     6481 cgtcgttggt gagcttcggc atccactcgc ccagccactc ctcccgtatg gccgcaatgc
     6541 gcccgctcac gccggcgcgc gcttccgcgg ggctgcggtc cttgccgtcc atggcctggc
     6601 tgagcgcgcg cagggtcaac gcggcgtcgc cgatgagccc gtgctgcgcg ggcacgtcct
     6661 tgttgagatc catggggtcc agggtggcgt gaatgatggt tctgcccgcg ggcatcggca
     6721 cgccgaagcc cgtgagggcg aagctgcagc cgattcccag aatcacgtcg gcttcgttga
     6781 gaaacgcttt cacgggccgc gggttggccc tgccgccgga gcccagcgag agggggtggt
     6841 tctcgggaaa cgcgctcttg ccctcgatgc tggtggttac cggaatgttc caggtctcgg
     6901 cgagcgcttt gagctccgcc caggccttgg cgtagtgaac gccctgacct gcgtagatga
     6961 cgggcctttc ggccttggcc agcacctccg ctgccgtggc gacggcgtcg gggtccggcg
     7021 cgctgcgggt tttgaagctc ggcgtgtggt gccagtcatc gggtacgtcc tcccggaaca
     7081 cgtccacggg aatttcgacg agcacggggc cgggccttcc ggtgcgcagc tggaagaagg
     7141 cgcggcgcat cacctcgggg accgctttgc ccatggtcag gggctcggcc cacttggtga
     7201 tgttgcgcat gttcagcgtc gagctgaaat tgggatagta gttggcgatg cggcggtgat
     7261 agcccgcggg tagcaccagg atcggcaccg attccgagta ggcctgggcg acgccgccaa
     7321 aggcgttctc ggcgccgggc ccgtgctgca tcgcgaacac gccgatcctg tctcctgacg
     7381 acaggcgtga gtaggcgtcc gccatgtgca gccccacccg ctcctgacgg acgatgaccg
     7441 tgcggatgtc ctccgccgcc gccgcctcga cgatcgggtt caccggatat gcgaacagca
     7501 cctccacgcc ctcggccttg agcgctttgg caatggcctg agcaactttc acctggtcac
     7561 ctcccatcct ggctggcgcc cggcttaccg ccgggccgtt tctcttgtat cttgcggatt
     7621 ctagctcact cgtatgaggt tgtagttggc cgactaccca tcgcagcggg gcatcgtgct
     7681 ctgggcggcc ccgtttctgg cggttctgct ctggtcggct gtgaacccga aggattacga
     7741 ggtatgggtc ctggaggtct cgccggcggt cgcgggggcg ctcatcatct ttctcacgcg
     7801 gcggcgcttt cccctcaccc ggctgacgta cgggctgatt ctcgtccatg cgctgatact
     7861 catcgtggga gggcactaca cgtacgccga ggtgcccctg ttcgatctcc tgcgggaacc
     7921 gctgggattc acgcgcaaca actacgacaa gctcgggcac ctggctcagg ggttcgtgcc
     7981 cgccatcgtc gcgcgggagg ttctgcttag gctttccccc ctggagcgtg gcggctggct
     8041 caaccttttc gtcgtctcgc tgtgccttgg gatcagcgcc ttctacgagc tgatcgagtg
     8101 gtgggtcgcg ctcttgagcg agcaggccgc agagtcgttt ctcggcaccc agggttacgt
     8161 gtgggatacc cagtccgaca tggggtgggc gttgctcggc gccgtgctgg cgctcgtcct
     8221 gcttgcgcgc taccacgacc ggcagctgct ggaacagggt ctcgcaggaa accatgactg
     8281 atcaggtgga ggagcagccg gcgggggcac ctgccgcgca gggtttctcc ggcaagacgc
     8341 cctggcgttc ctggtatgcc ctgggcgtgc tgttcgtggt ctacgtcttc aatttcatcg
     8401 accggtcgat cctcgggatc ctcaatcagg ccatcaaaga ggatctggct ctgtccgaca
     8461 cccagatggg ctttctgggc gggatcgctt tcgcggtctt ttacacgttt ctgggcatac
     8521 ccatcgcccg cctggccgac aggaccgtgc ggcgcaacct gctggccgtc tgcctggcca
     8581 tctggagcgt gatgacggcc ctctgcgggc tcgcttcgag cttcgcccac ctgctgctag
     8641 cacgcatcgg cgtggcggtg ggggaggcgg ggggcagccc gccctcgcac tccatgatct
     8701 cagacatgtt tcccgagtcg tctcgcgcca cggcgctctc catctacgct ttgggaattc
     8761 ccgtaggcac gatgatcggc aacctggccg gcggctggat caacgaggct ttcgactggc
     8821 gaacggcctt catcgtggta gggctccccg gggtggcgct ggcttttctc gtggttctga
     8881 cggtgcgcga gccggtgcga ggcgctgcgg acaacatgac ggcgagctcg gaagccgcgc
     8941 cgccggtcat gaccgtgttt cgctacctct ggtccctcaa gagctttcgc tacctgtcgc
     9001 tggccggcgc gctccacgcc ttcgtcgggt acggcgtggg ctactggttc ccggcgttct
     9061 tcatacgcag ccacggcctc ggcaccggag agatcggaac gtggctgttc tacctcggct
     9121 ttgcgggcat ggcgggcacc tttctcggtg gctacatggg cgaccgcatg gccaggcggg
     9181 acgtccgctg gtacgtgtgg ctgccgggga tcgcgacgct gctttccgtg ccgttctcga
     9241 tggtcgttta cctctacgac gactaccgcg tggcgttcct ggtggccatc gtgccgacgt
     9301 tgctgggagc ctactatctc ggccccactt tcgcgctgac ccagggactg gtagggctgc
     9361 gcatgagggc gctggcctcg agcatactgc tgttcattct gaacctgatc ggtcttgggc
     9421 tgggtcccca ggtcacgggc atcctcagcg atgggttcaa cgccttcggg gacctcggca
     9481 ccgactcgct gcgctacgcg ctgatctgcg tgctgctgct caacgtcgtg gccaccgtgt
     9541 actactggat ggcgggtcgg gacctggaga gggatctggc acggcgcggt gagctggacg
     9601 gctgagcggc cgtcaacccc gcgccgtgcg gtgcgcgatc agccgcccgc gttcatcgac
     9661 tgagcgatga cggcaaagcc gtcggtggct tccaggcgct tgccgaccac ggcctgatac
     9721 tcagccgagt tgtaccaggc tttggcggcg tccgtggatt cgaacttgat gatcaccgtc
     9781 tgcgggccgg gcgggttgcc ctcgacggtc tcggcgccta cgtcgaaaga gaccagctca
     9841 gccccgtagg tggccagagt aggcccggcg ccctgctggt actcggcgta aagggccggg
     9901 ttgttgacgg tgtactgagc gacgaagaat gcggccatgt ttggctcctc ccctttgcga
     9961 tgtgatgaga ccggctatgg tacccgatta ccccgcagcc acaattggcc gttgccgaga
    10021 ggggccgtct gcttcagatc gccatcagtc ggcggacgca gtcgggcgcg gtgcagtttc
    10081 cgtcgaggag gtccgtaagc gctgcgatga acggcgtggt cacgctccgg gctgcggcga
    10141 gctcggcaaa cattcgcgtg gcgcgaacgc cctccgcgac catgacgctg ccggccatgg
    10201 cttcctccag ggtctgcccg gcgcccagct tctggcccag ggaccggttg cggccgtggg
    10261 gtgacgatgc ggtgacgaaa agatcgccga tgccggccgg agtaaacgcc gtctccggcg
    10321 cggcccccag cgcggtgagc aggcgattac cttcggcaaa accggccgtg aagatcgccg
    10381 ccttcaggtt gtcgccgctt ccctcgtcaa gcccgtcggc gattccgcag cccagcgcga
    10441 tcacgttctt gaagcccccc cacaactccg cgccgcgcgg gtcgctggcc gccttcagcg
    10501 aaaaagtcga ggtggatagc tgctcggcga ggcggctcgc cagcgattcg tcttcgctgg
    10561 ccacgagcgc cgtggtgagc acgccgctgg caagctcggg cgcgatggtt gggccggtga
    10621 tcaccgcgag cgggttggtc ttgccggcgg ccgccagctt ggcctggatg gcatcggaaa
    10681 cgagctcctc gccggcgatg cccttggcga gatccagcac cacctgatcc ggcgtcagca
    10741 ggggtatgac gtcgtcggcg acgtgaagaa ccacgtggga gggcagggcg agcaccacga
    10801 gctccgcccc ggggaccgac tcgccaagct ccatggatac ctgcaggccg gtgaggtcga
    10861 tgccgtcgag gtatttgctg ttgctgcctt tggagcggat ggcgtcgaac acgtcttcct
    10921 ccaccgtcca tccgcggacg tcgtgcccgc ctagcagcca gacccgggcc agggccgtgc
    10981 cgaagttgcc gagcccgaga atcgcgatcg tggccatgtc tgccgcccct tggtcaccgt
    11041 tggatcgttg cctgttgtat gccaaagtta gccgatcagg caagacgccg gaggaagaac
//
LOCUS       DBNHKV010000060         2959 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1639440, whole genome shotgun sequence.
ACCESSION   DBNHKV010000060 DBNHKV010000000
VERSION     DBNHKV010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1639440"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="AC16PP_01755"
     CDS             <1..818
                     /locus_tag="AC16PP_01755"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6480376.1"
                     /translation="LPQMVMANVLGFPEEDIPQLAEWGNAQVAPFVYGKGHRNILTDE
                     QIKEQFELLDGFKEYVFDKVQEKRKDPQDDMISFLTQVEYKALGRKLTDMEVNGVVYA
                     MVIGGLETTQYALEEQAQLLCEREGIFDAIRQDREKLRQFTEEAMRLRSPTQGLSTRI
                     TSQDEVFQGVKVPKGSMLHLRWAAANIDEQEFECPYELQLDRKAASRHLTFSAGPRVC
                     PGAGISRLEQIIAWNRLLDRIDSLEYAPDNTFLHQPGIMLGTLELHLRFTKAQ"
     gene            complement(838..1425)
                     /locus_tag="AC16PP_01760"
     CDS             complement(838..1425)
                     /locus_tag="AC16PP_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480377.1"
                     /translation="MRRRLLMAAALVFPGMAVAEALRTECAGCETLRDFGNYGAAQLY
                     RAVGPMGPAVLNDRIWVVNPVTGLQAFVDLDTPTTLVYLFGAPIPVPDLLRTEINATW
                     GDGSVMETWILPNEVIGSMAEALISVDSAGTPPVNAELLDRLPGFGYGGGWYHAGSVG
                     SMTSTHYGAWSFLVRYDSYGRAVVTVYECAYASVC"
     gene            complement(1418..2290)
                     /locus_tag="AC16PP_01765"
     CDS             complement(1418..2290)
                     /locus_tag="AC16PP_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480378.1"
                     /translation="MMRIVLILSLAFSLVALWVATIRPPVEQRPEPAAGDAGRVASRA
                     PAVTAQPPVDERAEMIRQTAAPDEKNQADPGFDPMLVTGLGDFNAEEIERYNRLHLLP
                     FNPAIGRDCEDRPDPQLPGRSYTSCTTVRERPAHPYSELGDEVLLSLAEHDAAAALML
                     GRRVAGEDERLYWYLRAAALSEKSGPLMALAEQRYRDSMDLRLVRGELRRVPRPDRIA
                     VRVALDTVAGRMGDPRANPDRWRGVLRSLAADRAPTYLAQADALVAEFLGAMARTQRE
                     ITGSVQIQEIIDNA"
     gene            2568..>2959
                     /locus_tag="AC16PP_01770"
     CDS             2568..>2959
                     /locus_tag="AC16PP_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480379.1"
                     /translation="MQSRAPMGSLLRAFVGLLAITGCGIVSTVAAEERDASGNIEEIV
                     VTTKRGTVENVQEMSEAVTAFAGPSLEREFAVTLEDFNHAVPNVQLEHVGLFQAASSF
                     SMRGIGTAGIESFADPVVAVFVDDVYYS"
BASE COUNT          573 a          960 c          942 g          484 t
ORIGIN      
        1 cgctgcccca gatggtgatg gccaacgtgc tgggctttcc cgaggaggac attccccagc
       61 tggccgaatg gggtaacgcc caggtcgccc ccttcgttta tggtaaaggc caccgcaaca
      121 tcctgacgga cgagcagatc aaggagcagt tcgaactgct cgacggcttc aaagagtacg
      181 tcttcgataa agttcaggag aaacgaaagg atccccagga cgacatgatc tccttcctca
      241 cccaggtgga gtacaaagcg ctcggccgca agcttaccga catggaggtc aacggggtcg
      301 tttacgccat ggtgatcggc gggctggaga ccacccagta cgcgttggaa gagcaggccc
      361 agctgctgtg cgagcgcgag ggcatcttcg acgccatcag gcaggaccgg gaaaagctgc
      421 gtcagttcac cgaggaagcc atgcgcctgc gctctcccac ccagggcctt tccacacgca
      481 ttaccagcca ggacgaagtc tttcagggcg tcaaggtacc aaaaggctca atgcttcacc
      541 ttcgttgggc tgccgccaac atcgacgagc aagagttcga gtgcccctac gagctgcagc
      601 tggaccgcaa ggcggcgagc cggcacctca ccttctcggc aggcccacgc gtctgccccg
      661 gcgcgggcat ctcccggctg gagcagatca tcgcctggaa ccgtctgctg gatcgtatcg
      721 acagcctgga gtacgcgcct gacaacacgt tcctgcacca gccgggcatc atgctgggca
      781 ccctcgaact gcacctcaga ttcaccaagg cccagtaggc aaagaggacg cgctctctca
      841 gcaaacggac gcgtaggcac actcgtagac ggtcaccacg gcccgtccat aactgtcgta
      901 gcgcaccaag aaagaccacg cgccgtaatg agtactcgtc atgctcccga cgctgcccgc
      961 gtgataccac cctcccccgt acccgaagcc tggcaggcgg tccagcagtt ccgcattgac
     1021 cggcggcgtc cccgcggagt ctaccgaaat cagcgcttcg gccatggaac cgatcacctc
     1081 gttgggcagg atccaggtct ccatcacgga gccgtcgccc caggttgcgt tgatctcggt
     1141 acgtaacaga tcgggcactg ggatgggcgc cccaaacaga tagaccagcg tcgtcggcgt
     1201 atccagatct acgaacgcct gcaggccggt caccgggttg acgacccaga tccgatcgtt
     1261 cagaacggcc gggcccatgg ggcctacggc gcgatacagc tgcgccgcgc cgtagttacc
     1321 gaagtcgcgc agcgtttcgc atcccgcgca ttcggtgcgc agggcctcgg cgacagccat
     1381 tccagggaac acaagcgcag cggccatcag gagacgtcta cgcattgtcg atgatctcct
     1441 ggatctgcac gctgcccgtg atctcgcgct gggttcgcgc catggcgccg agaaactcgg
     1501 ccaccagggc atcggcctgg gcgagatagg ttggcgctct atcggcggca agggagcgca
     1561 gcacccctcg ccaccggtcc gggttcgccc tggggtctcc catgcgccca gccaccgtgt
     1621 ccagggcaac gcgaacggct atgcggtcgg gtcggggaac gcggcgcaac tcccctcgca
     1681 cgagccgaag gtccatcgag tcacggtaac gctgctcggc cagggccatg agcggcccgc
     1741 tcttttctga cagcgctgca gcacgcaggt accagtaaag gcgctcgtct tctccggcga
     1801 cgcggcggcc aagcatcagg gctgcggcag catcgtgctc ggcgaggcta agcagcacct
     1861 cgtcgcccag ctcgctgtaa gggtgagcgg gtcgttcgcg gacggttgtg caactggtgt
     1921 aggagcgccc gggcaactgc gggtcgggac gatcctcgca atcacgcccg atggctggat
     1981 tgaatgggag caggtgaagg cggttatacc gttcgatctc ttcggcgttg aagtcaccga
     2041 gacccgtcac cagcatcgga tcgaaacccg gatcggcctg gttcttctca tcgggagcag
     2101 ccgtctgccg aatcatttcc gctcgttcat cgacaggagg ctgggccgtc accgccggcg
     2161 cccgggacgc cacccgcccg gcatcgccgg cggcgggttc aggacgttgc tcgacaggcg
     2221 gacggatggt cgccacccat agcgcgacga gactgaaagc cagactcaag atcaggacga
     2281 ttcgcatcat gcgggacctc ttgcgttacc atgtccatgg ggcaaatcat cgtcccgcga
     2341 gctcagcgcc cggagttaga gccgggatcc cccgcccgcg tgcgacggtt gctgcgacga
     2401 ccggccaaaa gaacacttgt cgacggccgt cgttcgccca gcggccgtgt gggcaacgcg
     2461 gccgttgaca acccccgcgg tcggagggca cagtctgccg agggcggtgg aaggctcaag
     2521 ggaagagccc gtctaccggg gtcagagaga accgagggga gatcaccatg caatcacgag
     2581 cacccatggg gtcgctgttg cgggcatttg tcggcctgct ggcgattacc ggctgcggca
     2641 tcgtcagcac cgtcgctgcc gaggagcggg acgcgtcagg caacatcgaa gagatcgtcg
     2701 tcacgacgaa gcgcggcacg gtggagaacg tgcaggaaat gtccgaagcc gtcacggcct
     2761 tcgcgggacc gagcctggag agggagttcg ccgtgacgct ggaggacttc aaccacgccg
     2821 tgcccaacgt gcagctcgag cacgtcggcc tgttccaggc cgcgtcgtcg ttcagcatgc
     2881 gtggtatcgg caccgccggg atcgagagct ttgccgaccc ggtggtggcc gtgttcgtgg
     2941 acgatgtgta ctactccag
//
LOCUS       DBNHKV010000061         4255 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1850920, whole genome shotgun sequence.
ACCESSION   DBNHKV010000061 DBNHKV010000000
VERSION     DBNHKV010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1850920"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="AC16PP_01775"
     CDS             complement(<1..1123)
                     /locus_tag="AC16PP_01775"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6480380.1"
                     /translation="MNDPESIANLEARMAWEQERAVPPEGFPALPPLPPGRYTSPAFF
                     DLERRHLWSKVWLYAAHDSQLPEPGSFLLLDIPDAPIFLIRGRDASVRAFYNVCSHRG
                     APLVREASGQVARLRCTYHQWTYDTDGRLIAVMDERDFAEPFDRSCLGLKEVRCEQWG
                     RWIFINEDPEAQPLLEWLDMIAAEFEQFDIERLRPAAIKSYELECNWKLTMDAFLEVY
                     HIQGIHPKTVGPSLDHRGAVMGLLPNGHTRMTCPTRMSRTETADTRGYSDSAGEAMPA
                     IPAGEIARNYHVSHNVFPNLITPVGDTARQFLMFWPLSLTRTRVDVVHFGLDWGEGER
                     PSGWDGLLAFWDVVMAEDLQFLEWQQKAVLSPGFQGYRLS"
     gene            complement(1147..1632)
                     /locus_tag="AC16PP_01780"
     CDS             complement(1147..1632)
                     /locus_tag="AC16PP_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6480381.1"
                     /translation="MNFDDYHAIVRRRYEYALGIDTRDWVLYRSIFTDEITMDFSSYN
                     GRPASGMAADDWVAGCKVLFAGLDATQHVMSNPMVDVDGDIARLRMYMKAEHFLRNDQ
                     GGMDFTIGGYYDDRLVRTDEGWKMQAVTLNVFWSRGNRQIMALAAEIGKGKLEEASSG
                     S"
     gene            complement(1647..2378)
                     /locus_tag="AC16PP_01785"
     CDS             complement(1647..2378)
                     /locus_tag="AC16PP_01785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6480382.1"
                     /translation="MNDNAIALVTGGATGIGAEVCRQLAAAGARVAICDVNEADGAPL
                     AESLGGEFLFCDVADLASVEGAVAGCCARLGVPDYVHLNAGVMTVGRYDAYLALEDVS
                     VEQYRRIIGVNVDGVFNGIKALLPPMRQKGGAITVTASIAGFAPMAIDPLYGATKAAL
                     ISLVRSVAAANEGGSVRINAICPGVVDTAIVPDAMSGTIPMMPPSAMAAEILDLLSDG
                     PSGEIRVKLNEETPAFHVEPSTLGR"
     gene            2428..3624
                     /locus_tag="AC16PP_01790"
     CDS             2428..3624
                     /locus_tag="AC16PP_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6480383.1"
                     /translation="MDHHRPNVTTTIDRSPTRPDKAADTTLKALFLLTAVLMVGYGAV
                     FSLLAEIRDAFGFTPTGIGLIAGSAFAAGFFAQLWLARYADRGYGAIMLRAGLAISAL
                     STLWMAVADTLAAWIAARALLGFGAGCVRPAVRRLALTADPANAGHAMGRLTAYETAG
                     FLLGPVLAALLDRWAGLASTFIVMTGVLALLAPMVWRVSIPGASRPTTGPVIGTLLRN
                     PYMQACLALGIAFWITIGVFEAVWAIFLADLGASQMFIGLTMSLFGIPMIFIAPRAGS
                     LAQHRGPLRVASVSMAVATVCMVLYGFLESLWLLCVPLGIHAIADAYTMPASQLAVGR
                     ASGEGALAAGQGLFGATGMAVGAVTAAGGGVLYQSLGPSGLWWGSAGAMIVCLLISWW
                     RSPRAT"
     gene            complement(3691..4134)
                     /locus_tag="AC16PP_01795"
     CDS             complement(3691..4134)
                     /locus_tag="AC16PP_01795"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6480384.1"
                     /translation="MPGESCRVGYRHAMDAVPGSVWRTLADFGSLLSWVAGGDEGRIE
                     LSGEGIGMTRDLWLPSVGRVQHRLDVLDEAGRCLTYSLAEGRPLGMRSYSVTVSVEAA
                     GSGCTLAWQAAFVPEPGADSDAMAENLRRAYVDMSRRLDVLLRAA"
BASE COUNT          685 a         1455 c         1348 g          767 t
ORIGIN      
        1 agctcaggcg gtagccctga aatcccggcg agagcacggc cttctgctgc cactccagaa
       61 actgcagatc ctcggccatc accacgtccc agaaggcgag caggccatcc catcccgagg
      121 gacgctcgcc ctcaccccag tccaggccga aatggaccac gtccacccgg gtccgcgtga
      181 gcgaaagggg ccagaacatc agaaactgcc tggccgtgtc gccaacgggc gtgatgagat
      241 tcggaaagac gttgtgggag acgtgatagt tgcgagcgat ctcgccagcc ggaatggcag
      301 gcatcgcctc gcctgctgaa tcgctgtatc cgcgcgtgtc ggcggtctcg gtgcgggaca
      361 tccgggtcgg gcaagtcatg cgcgtgtgcc cgttgggaag cagccccatg acggcgcccc
      421 ggtggtcgag cgatggcccc acggtcttcg ggtggatgcc ctgtatgtgg tagacctcca
      481 ggaacgcatc catggtcagc ttccagttgc actcgagctc gtagctcttg atcgccgccg
      541 ggcgcaggcg ctcgatgtcg aactgctcga actcggcggc gatcatgtcg agccattcca
      601 gcagcggctg tgcttcgggg tcctcgttga tgaatatcca ccgaccccac tgctcgcagc
      661 gaacttcctt caggccgagg caggatcgat cgaagggctc cgcgaagtcg cgctcgtcca
      721 tgacggcgat cagtcgtccg tccgtgtcgt acgtccactg gtggtaggtg cagcgaagcc
      781 gggccacctg accggaggcc tcccgcacca acggcgctcc gcgatgcgag cagacgttgt
      841 aaaacgcgcg gacgcttgca tcgcggccac gaatcagaaa gatcggcgca tcggggatgt
      901 ccagcaggag aaagctgccg ggctccggca gctggctgtc gtgggccgcg tagagccaga
      961 cctttgacca caggtgacga cgctcgagat cgaaaaatgc cgggctggtg tagcggccgg
     1021 gcggcagagg cggcaacgcc ggaaagccct cgggcggaac cgcccgttcc tgctcccagg
     1081 ccatgcgcgc ctcgaggttc gcaatggact ccggatcgtt catcggccgt actccctaac
     1141 gcgctgtcac gaacccgacg acgcttcttc gagcttgccc tttccaatct ccgccgcgag
     1201 cgccatgatc tgccgattgc cgcggctcca gaagacgttc aacgtcaccg cctgcatctt
     1261 ccagccctca tccgtcctga ccagccggtc gtcgtagtag ccgccgatgg tgaaatccat
     1321 gccgccctga tcgttgcgca gaaagtgctc ggccttcatg tacatgcgca gccgggcaat
     1381 atccccgtcg acgtcgacca tcggattgga catcacgtgc tgggtggcat cgagcccggc
     1441 aaacagaacc ttacagccgg ccacccagtc gtcggccgcc atgccggatg cgggtcggcc
     1501 attgtaggaa gagaagtcca tggtgatctc gtcggtgaag atgctgcgat agagcaccca
     1561 gtcccgagtg tcgatcccca gcgcgtactc gtagcggcga cggacgatgg catggtaatc
     1621 gtcgaaattc atgatggcct gcccgttcag cgacccaagg tgctcggctc gacgtggaag
     1681 gcgggcgtct cctcgttgag cttgacgcgg atctcgccgg aggggccatc gctgaggagg
     1741 tccagtatct cagccgccat ggcggacggt ggcatcatcg gtatcgtgcc ggacatcgca
     1801 tcgggcacta tggcggtgtc caccaccccc gggcagatgg cgttgatacg cacgctgccg
     1861 ccttcgttgg ctgccgcgac gctgcgcacg aggctgatca gggcggcctt ggtggcgccg
     1921 tagagcggat cgatggccat cggggcgaag ccggcaatgg aggccgtcac cgtgatggct
     1981 ccgcccttct gcctcatggg aggcagaagc gccttgatac cgttgaagac gccgtccacg
     2041 ttgacgccga tgatccggcg gtactgctcc acgctgacgt cctcgagcgc aaggtaagca
     2101 tcgtagcggc ccacagtcat gacgccggca ttcaggtgta cgtagtcggg caccccaagc
     2161 cgggcacagc agccggcgac cgctccctcc acgctcgcca ggtccgccac gtcgcagaac
     2221 agaaactcgc cgcccaggga ctcggccagc ggcgcgccgt ctgcctcgtt gacgtcgcag
     2281 atggccaccc ttgctcccgc cgcggcgagc tgtctgcaca cctcggcgcc gataccggtt
     2341 gcccctccgg tcaccagcgc gatcgcgttg tcgttcaccc ttcccctcct cgcatgatct
     2401 ttgctttccg ccctagattg cggttcgatg gaccatcacc gaccaaacgt taccacgacg
     2461 atcgaccgtt cacctaccag acctgacaag gcagccgata ccacgctcaa agcccttttc
     2521 cttctgaccg ccgtcctgat ggtgggctac ggggcggtgt tttccctgct ggccgaaatc
     2581 cgcgacgcgt tcggcttcac gcctacgggc atcgggctga tcgcaggctc tgcgttcgcg
     2641 gccggtttct tcgcccagct gtggcttgcg cggtacgcgg atcgcggtta cggggcgatc
     2701 atgctgcggg caggtctcgc gatctcggcg ctgagcaccc tgtggatggc cgtcgccgac
     2761 accctcgctg cgtggattgc cgcgcgcgcc ctgctggggt tcggcgccgg ctgcgtccgc
     2821 cccgccgtac gtcgtctggc actcaccgca gaccccgcca atgccggcca cgccatggga
     2881 cgtttgaccg cctacgagac ggctggcttc cttctcggcc ccgtgctcgc cgcgctgctc
     2941 gaccgctggg ctggcctggc gtccaccttc atcgtgatga cgggcgtcct ggcgctgctc
     3001 gcgcccatgg tgtggcgcgt gagcattccc ggggccagcc gaccgacaac gggtcccgtc
     3061 atcggcacgc tgttgcgcaa tccttacatg caggcatgtc ttgctctcgg catcgcgttc
     3121 tggatcacca tcggcgtctt cgaagcggtc tgggccatat ttctcgccga tctgggggcc
     3181 agccagatgt tcatcgggct caccatgagt ctcttcggga tcccgatgat cttcatcgcg
     3241 ccccgtgccg gctccctggc ccagcaccgg gggcctctcc gggtggcctc ggtcagcatg
     3301 gcggtcgcaa ccgtatgcat ggttctctac gggtttctgg agtcgctgtg gctgctatgc
     3361 gttccgctgg gcattcacgc catcgccgac gcctacacca tgccggcgag ccagctcgcg
     3421 gtcggacggg caagcggtga gggggccctg gcagcgggcc agggcctgtt cggcgccacc
     3481 gggatggccg tcggcgccgt gaccgccgca ggcggcgggg tcttgtatca gagcctcggc
     3541 ccgagcgggc tgtggtgggg gtctgccggg gccatgatcg tctgcctgct catatcctgg
     3601 tggcgcagcc ctcgagcgac ctgatcacgg catcgaactt atcgccctcg tctttgccat
     3661 tgccgctgat gcaggcttgc aggcgacggg tcacgctgct cgcagcagca catcgagccg
     3721 ccgagacatg tcgacgtagg ctcggcgcag gttttcggcc atggcatccg aatcggcgcc
     3781 aggctccgga acgaacgcag cctgccacgc cagcgtgcag ccggatccgg ccgcttccac
     3841 gctcacggtc accgagtagc tccgcatgcc cagcggacga ccctcggcga ggctgtaggt
     3901 gaggcagcgg ccggcctcgt cgaggacatc gagtcgatgc tgcacccgac ctacgctggg
     3961 gagccacaag tcccgcgtca tgccgatgcc ctcaccggac agctcgatac ggccctcgtc
     4021 gccgccggct acccagctca gaagcgatcc gaaatccgcc agcgtccgcc agacggaacc
     4081 aggcacggcg tccatggcgt ggcggtatcc gacccgacag gattcacccg gcatcgagcg
     4141 tcaccttggc tcgcgcccgg gattggcgaa cggccccgat atcattctcg tccgaatcgt
     4201 ggcgcggcga ctgcggctgc atcccttgcg tctcccattt cccagtgcgc ccatt
//
LOCUS       DBNHKV010000062         4177 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1143030, whole genome shotgun sequence.
ACCESSION   DBNHKV010000062 DBNHKV010000000
VERSION     DBNHKV010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1143030"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="AC16PP_01800"
     CDS             complement(<1..560)
                     /locus_tag="AC16PP_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480385.1"
                     /translation="MRIVASLVAMTGYLLFCVMLFRFGYLDTGPPVFYTLLTLWSVVL
                     AALVVFDVPSARFDRGYLTFFKALWCNVGVVATALVVPASLRPLMLVVPLFGVLYTAL
                     HLERKHVLAVGTVTWFTYVLGALIQNDMRFIDETSPGLSTEGMVVVAFALMLIVMTVM
                     GGEVTALRNAFAHRRERLKQAMERLS"
     gene            complement(740..2218)
                     /gene="glpK"
                     /locus_tag="AC16PP_01805"
     CDS             complement(740..2218)
                     /gene="glpK"
                     /locus_tag="AC16PP_01805"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIR6480386.1"
                     /translation="MSHLLALDQGTSSSKAIIFNLTGRVVGVGQHEFDNTFPADGWVE
                     QDPEVLWRTTLLAGREALASAGLKGEDIAAIGITNQRETTLVWDRQTGRCPHNAIVWQ
                     DRRTADRCELMRLDGLAEAVAETTGLLIDPYFSATKLAWILDNVSGAREQAESGALAF
                     GTVDTFLVWRLTKGRHHATDASNASRTMLFDIAGQTWSERLLNYFNIPAAMLPAVRDS
                     ADDFGVADPEWFGAPIPICGIAGDQQAALIGQGCFNPGMAKSTYGTGCFAMINTGAEQ
                     LRSQQQLLTTVAYRLDGEITFALEGSIFSAGVAVKWLRDQLGLVVDAADTEAAAIRAQ
                     GDTAGVFLVPAFTGLGAPHWAPEARGLVSGLTLATTRDQLITATLQAVAFQSHELIAA
                     MASDGAVVDNLRVDGGMVVNDWLCQCLADVVNLPVERPEITETTALGAAILAALGAGL
                     VEDLDSAARLWNLEREFLPAMDPARRESLLKGWRAAVARTLL"
     gene            2724..>4177
                     /locus_tag="AC16PP_01810"
     CDS             2724..>4177
                     /locus_tag="AC16PP_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008100729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HIR6480387.1"
                     /translation="MQSDNQPISGTATPSDPQATATAMATAQPLPPSIAATAPGEIKV
                     IKRNGAVVPYNDDKIAVALTKAFLAVEGGTAAASPRIRELVAELTLQVSATFKRRFPS
                     GGTIHIEDIQDQVELVLMRSGEHKVARDYVLYREERARARAADVVQPASNALNVVQED
                     GSREPLDLDRIRKLVGEACVSLSDVEADRIIDEAMSNMYDGISAKDVATALQIAARTL
                     VEEEPNYTYVTARLLLDELRTEAFRFLEVNAEGEHRATQSEVGGYYPAALAAFIEKGI
                     ELQLLDPVLATFDLAALGGAIKSERDLKFTYLGLQTLYDRYFIHSNDVRFELPQVFFM
                     RVAMGLAVQEDDINARAIEFYDLLSSFDYMSSTPTLFNSGTLRPQLSSCFLTTVPDDL
                     ECIYGAIRDNAMLSKWAGGLGNDWTPVRALGSYIKGTNGKSQGVVPFLKVVNDTAVAV
                     NQGGKRKGAVCSYLETWHLDIEEFLELRKNTGDDR"
BASE COUNT          816 a         1355 c         1315 g          691 t
ORIGIN      
        1 tcagatagcc tttccatggc ctgcttgagc cgctcgcggc ggtgggcgaa cgcattgcgc
       61 agagccgtca cctcaccacc catgaccgtc atcacgatga gcatgagggc gaaggcgacc
      121 accaccatgc cctccgtgga gaggccgggc gaggtctcgt cgatgaagcg catgtcgttc
      181 tggatcagcg cgccgaggac ataggtgaac caggtcaccg tccccaccgc gaggacgtgt
      241 ttgcgctcga gatgcagcgc cgtgtagagc acgccgaaca gcggcacgac cagcatcagg
      301 ggccgcaggg aagccggaac gacaagggcg gtcgcaacca cacccacgtt gcaccacagc
      361 gctttgaaga acgtcaggta tcccctgtcg aaccgtgcgc tgggcacgtc gaaaaccacc
      421 agagccgcca ggacgacgct ccagagggtc agcaacgtgt agaacaccgg cggaccggta
      481 tccagataac cgaaacgaaa cagcatgacg cagaaaagca gatatcccgt catggcgacg
      541 agagatgcca ctattcgcac cagcttcgtt ccggcgattt gaagcgcgga aagcaatgct
      601 attccctgca aatctcctag ctgagctgaa ttctagggaa tctgcacgaa gccaatacag
      661 gacgtggtac cgccttggcg gcgggaatcg cgcgccaggc ccgccttttt ggctaaaacg
      721 tgacgcggga caaactttct cagaggagcg tcctggccac ggcggcgcgc cagcccttga
      781 gcaggctctc ccggcgtgcc gggtccatgg caggcagaaa ctcccgctcg aggttccaaa
      841 gccttgccgc cgagtccaga tcctcgacca acccagcgcc aagcgccgcc aggatggcgg
      901 ctcccagcgc cgtggtctcg gtgatctcgg gacgctccac cgggaggttc accacatcgg
      961 caaggcactg gcacagccag tcgttgacca ccatgccacc gtccacgcgg aggttgtcga
     1021 cgacggctcc atccgaggcc atggcggcga tgagctcgtg gctctgaaag gccacggcct
     1081 gcagcgtggc ggtgatcagc tggtcgcggg tggtcgccag ggtgagcccg gacaccagac
     1141 cccgggcttc gggcgcccaa tgaggtgctc cgaggccggt gaacgccggc accagaaaga
     1201 cgcctgcggt atctccctgg gcgcggatgg ccgcggcctc cgtgtccgcc gcatcgacaa
     1261 ccagcccgag ctggtctctc aaccacttga ccgcgacccc tgccgagaaa atgctgccct
     1321 cgagcgcgaa agtgatctcc ccgtcgaggc gataggccac ggtagtgagc agctgctgct
     1381 gcgagcgcag ctgctctgcc ccggtgttga tcatggcaaa gcagcccgtc ccgtaggtgc
     1441 tcttcgccat tccggggttg aagcagccct gcccgatcaa cgccgcctgc tggtcgccgg
     1501 caatcccgca gatcggtatc ggcgcgccga accactccgg gtcggccacg ccgaagtcat
     1561 ccgcgctgtc cctgaccgca ggcagcatcg ccgcggggat gttgaagtag ttgagcagcc
     1621 gctcgctcca cgtctgcccg gcaatgtcga acagcatggt gcgtgacgcg ttggaggcat
     1681 cggtggcgtg gtgccggccc ttggtaagcc gccagacgag gaacgtatcg accgtgccga
     1741 acgccagcgc gcccgactcg gcctgctcgc gcgcgccgga aacgttgtcg agaatccagg
     1801 cgagcttcgt tgcggaaaaa taagggtcga tgagcaggcc cgtggtctcg gccaccgctt
     1861 ccgccaggcc atcgaggcgc atcagctcgc agcgatcggc ggtgcggcgg tcctgccaga
     1921 cgatggcgtt gtgcgggcag cggccggtct ggcgatccca gaccagggtg gtttcccgct
     1981 ggttggtgat gccgatggcg gcgatgtcct ctcccttcag acccgcgctg gccagcgctt
     2041 cgcgccccgc cagcagcgtg gtccgccaca gaacttccgg gtcctgttcc acccagccgt
     2101 ccgccgggaa ggtgttgtcg aactcgtgct ggcctacgcc caccacccga ccggtgaggt
     2161 tgaaaatgat ggctttggag ctggaggttc cctggtcgag ggcgagtaga tggctcatga
     2221 ggagagtccg gtaccacgaa aaccggccac aatatatagt ggtcgccccg gttcggcgag
     2281 aggcgcgacg gcagaaaatg tccgccgtcc gggtggccca cagcatatcc acaacgtatc
     2341 cccagctaat tccttgcaac cctcctgcaa tcgaattatc ttgtgtggtc ttgggttcga
     2401 atcggctgaa aaacccaaca tgttgcggtt atgagcattg actcggtcaa tttattgact
     2461 gccagtctca ggggagccag tcgccgcagc agcaagtcag cgaagcgcct gaccacgcga
     2521 gccatacaga aatctgttcg gaggggacct atttctgccg gagccgctaa tactaggtat
     2581 tgtgtttcca cggtcgccgc cgcacgtccc tctcgcggca cgaccggggc actgcgaccg
     2641 tggaaacctg cggtcgaccc ggtgcgagcg agttcaacgg ttcaggcgtt tttgaaacag
     2701 cgagcaggag cgcacaacta gcaatgcaaa gcgacaacca acccatttcc ggcactgcca
     2761 ccccgtccga tccgcaggcg acggcgaccg ccatggcaac ggcccagccg ctgcccccga
     2821 gcatcgccgc cacggccccg ggggagatca aggtcatcaa gcgcaacggc gcggtcgtgc
     2881 catacaacga cgacaaaatc gccgtggctc tcacgaaagc attcctggcc gtcgagggcg
     2941 gcaccgccgc cgcctcaccg cgaatcagag agctcgttgc cgagctgacc ttgcaggtct
     3001 cggccacgtt caagcgccgg ttcccgtccg gggggaccat ccacatcgaa gacattcagg
     3061 atcaggtgga gctggtcctc atgcgctccg gtgagcacaa ggtggcgcgc gattacgttc
     3121 tctaccgcga ggagcgcgcg cgtgccaggg ccgcagacgt ggtgcaacca gccagcaacg
     3181 ccctcaacgt ggtgcaggaa gacggcagcc gcgagccgct cgacctggat agaattcgca
     3241 agctggtggg cgaagcctgt gtctcgctca gcgacgtcga ggccgatcgg atcatcgacg
     3301 aggccatgtc caacatgtac gacggcatct cggccaagga cgtcgcgacg gccctgcaga
     3361 tcgcggcccg aacgctggtc gaggaagagc cgaactacac ctacgtgacc gctcgcctgc
     3421 tgctcgacga gctgaggacc gaggcgttcc gttttctcga agtcaatgcc gaaggcgagc
     3481 acagggcgac ccaatccgag gtcggcggct actatcccgc ggcgctggcc gccttcatcg
     3541 agaagggcat cgagctgcag ctcctggatc cggtgctggc gacattcgac cttgcggctc
     3601 tcggcggagc catcaagtcg gaacgggacc tcaagttcac ttacctgggc ctgcagacgc
     3661 tttacgaccg ctacttcatc cactcgaacg acgtgcggtt cgagctgcct caggttttct
     3721 tcatgcgcgt cgccatgggg ctcgccgtgc aggaagacga catcaacgcc cgagccatcg
     3781 agttctacga cctgttgtcg tcgttcgact acatgagctc cacgccgacg ctgttcaatt
     3841 ccggaacgct ccggccgcag ctgtcgtcgt gcttcctcac gacggtgccg gacgatctgg
     3901 aatgcatcta cggagcgatc cgggacaacg ccatgctgtc gaagtgggcg ggcggtctcg
     3961 gcaacgactg gactccggtg cgggcgctgg gctcctacat caagggaacc aacggcaagt
     4021 cccagggcgt cgtgcctttc ctaaaggtgg tcaatgacac ggcggtggcc gtgaaccagg
     4081 ggggcaagcg caagggcgcg gtatgttcct atctggagac ctggcacctg gacatcgagg
     4141 agttcctcga gctgcgcaag aacacgggcg acgaccg
//
LOCUS       DBNHKV010000063         3052 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1568320, whole genome shotgun sequence.
ACCESSION   DBNHKV010000063 DBNHKV010000000
VERSION     DBNHKV010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1568320"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="AC16PP_01815"
     CDS             complement(<1..128)
                     /locus_tag="AC16PP_01815"
                     /inference="COORDINATES: protein motif:HMM:NF014053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6480388.1"
                     /translation="MTTYDVAIIGGGHNGLVCAAYLAGAGLDVIVLEKRGVVGGAAV"
     gene            complement(165..965)
                     /locus_tag="AC16PP_01820"
     CDS             complement(165..965)
                     /locus_tag="AC16PP_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480389.1"
                     /translation="MEATPLLMGIRARHALWFDCAMGFAALAVVCLLMLAMDERALNG
                     VNVWAKPLKFSISLAVYFATLAWFAPLMPDGYFAGTGGRWLSAIPVACAVFEIVYIIV
                     QAALGEPSHFNVSTTFHSTMYTLMGGGAFILVAICLWMGLIILSIHRMSNPYALSVGL
                     ALVLTFVLGGGFGAYLGGQTGHWVGGTLSDADGLWLVNWSRDVGDLRVPHFFGMHAMQ
                     ILPALATLVPHRMTRDGANVAVLAMTLLYAAFAGWTFQQAVSGIPFAG"
     gene            complement(1007..1453)
                     /locus_tag="AC16PP_01825"
     CDS             complement(1007..1453)
                     /locus_tag="AC16PP_01825"
                     /inference="COORDINATES: protein motif:HMM:NF025473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABA4-like family protein"
                     /protein_id="HIR6480390.1"
                     /translation="MLDTIFSATNALAMIGWAGLILLPGRRFVVQILARVLIPAIIAV
                     IYIGLMVGHVGSFPEGGGFGSLAGIATLFSVDALLLAGWIHYLAFDLFVGSWEVTDSR
                     KEGIPHLLLVPCLVLTFMAGPAGLALYFAIKYGRRSLASEAAQPSQ"
     gene            1745..2059
                     /locus_tag="AC16PP_01830"
     CDS             1745..2059
                     /locus_tag="AC16PP_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480391.1"
                     /translation="MSAVNIIRIVALVLAVVGAFVTIPYAAAALVILGLVAGFMGVDE
                     ERRLMVLLMAVALAVVADGLGPIPAVGDYLTAILTNLSGVFDGLAIAVILTAIYERIT
                     QG"
     gene            complement(2152..2628)
                     /locus_tag="AC16PP_01835"
     CDS             complement(2152..2628)
                     /locus_tag="AC16PP_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6480392.1"
                     /translation="MTNRDQTSTASPLEHASGPALLRELVHATYWLDDGLQAYMKEHA
                     GLSLPRAQSMMMVYIAEGVDSPSDLARRLRVSKQAIRQGLKELMAKDMVTLEPDPGNR
                     RQKVVRFTKHGRALRQVAWEGLQNLERELGRRLGGDRLAALHDALFLDWGDVPGEP"
     gene            complement(2625..>3052)
                     /locus_tag="AC16PP_01840"
     CDS             complement(2625..>3052)
                     /locus_tag="AC16PP_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6480393.1"
                     /translation="MGGGNELAFFWFRDAKDAQPGVASAAHLVGSGEGSITSAHGSMN
                     HVAFDVAPDQIEAYREKLVSRGVNCTPLVNHDDVVTGEESRRGSGISAKTWLRSFYFY
                     DPDGIMLEFCATLNTGSPEVELPVNAAGIKANGEPITGA"
BASE COUNT          564 a          966 c          990 g          532 t
ORIGIN      
        1 acggcagccc cgcccaccac gccccgtttc tccaggacga tcacgtccag gccggcgccg
       61 gccagataag ccgcgcagac cagcccgttg tgaccgccgc cgatgatcgc tacgtcgtac
      121 gttgtcatgc cctccggcgc ccccgtggac gggcactcta atcgctagcc ggcgaacggt
      181 ataccgctca cggcctgctg gaacgtccag ccggcaaagg cggcgtaaag cagggtcatg
      241 gcgagcaccg cgacgttcgc cccgtcccgg gtcatgcggt gagggaccag ggtggccaga
      301 gccggcagga tctgcatggc gtgcatcccg aaaaagtgcg gcacccttag atcgccgacg
      361 tcccgcgacc agttcacaag ccaaagaccg tcggcgtcgg agagcgtgcc gcccacccag
      421 tgcccggtct ggccgcccag gtaggcaccg aagccaccgc cgagaacgaa cgtcagcacg
      481 agggcgaggc ccaccgagag cgcgtagggg ttcgacatcc ggtggatgct caggatgatc
      541 aggcccatcc acaggcaaat ggcaacgagg atgaaggcgc cgcctcccat cagcgtgtac
      601 atcgtgctgt ggaacgttgt ggagacgttg aagtgcgatg gctcacccag ggccgcctgc
      661 acgatgatgt agacgatctc gaagacggcg caggccacag ggatggcgct gagccagcga
      721 ccgccggttc ctgcgaaata gccgtcgggc atgagcggcg cgaaccaggc cagcgtggcg
      781 aagtaaacgg cgagggagat agagaacttc aggggcttgg cccagacgtt taccccgttc
      841 agggcgcgct cgtccatggc aagcatcagc aggcagacga cggccagggc ggcaaacccc
      901 atggcgcaat cgaaccagag ggcgtggcgc gcgcgaatgc ccatgagcag cggggttgcc
      961 tccaccggcg attcccctgc caataccggc tgccgccccc tttcgctcat tggctcggct
     1021 gtgctgcctc gctcgccagc gatcggcgtc cgtacttgat ggcaaagtaa agagccaacc
     1081 cggcgggacc ggccatgaag gtcaagacca ggcagggcac caggagcagg tggggaatgc
     1141 cttctttccg ggaatccgtc acctcccagg aacccacgaa cagatcgaat gccaggtagt
     1201 gaatccaccc ggccaggagc agtgcgtcca cgctgaagag cgtcgcgatg cccgccagcg
     1261 agccgaaccc gccaccctcc gggaagctgc ccacatggcc gaccatcagg ccgatgtaga
     1321 tcacggcgat gatggccggg atcagcaccc gggccagaat ctgaaccacg aaccgccggc
     1381 ccgggagcaa gatcaagccg gcccaaccga tcatcgcgag tgcgttggtg gcggagaata
     1441 tggtgtccag cacggccgac ctcctgatta gcggattggc tgtttgggga tctccgattc
     1501 tcccggctcg gaagtccgat ctcaacccga gaccggattc atggcgctct ggtaacccgg
     1561 tcggtggcag atcttttccg cggcggcatc cgaagcatcg ccgccgtcga ataacgagca
     1621 gttcaacgat ctgttcgcgg ccgtgtgacg tgttgtgcta aattcgagtt gcgcctgcag
     1681 tccgagcgcg aggaaaacaa tgcagtaaac gtgaattcac agtgagggga gctcgctaat
     1741 gagtatgagt gctgtaaaca ttattcggat cgttgcgctg gttctggcag ttgtcggcgc
     1801 gtttgttacc attccctatg ccgccgccgc cctggtcatt ctcggcctgg tagcggggtt
     1861 catgggtgtg gacgaggaac gtcgcctcat ggtgctgttg atggccgtgg cgttggcagt
     1921 ggtggccgac ggtttggggc caattccggc tgtcggtgat tacctgaccg ccatactgac
     1981 caacctcagc ggtgtgttcg atggcctggc cattgcggtc atcctgacgg ccatctacga
     2041 gcgcatcacc caggggtaac gcggcaactg gtttcgcggg tgtctggcga tgaccgttgg
     2101 attgcccgcg gcaaccttca ctaactgaga cggctcccgg atgagccctg atcagggctc
     2161 tccggggacg tcgccccaat ccaggaaaag ggcgtcatgc agggcggcca ggcggtcccc
     2221 gccgagtcgt cgccccagtt cccgttccag attctgaagt ccttcccagg ccacctgtcg
     2281 cagcgcgcgg ccatgcttgg tgaagcggac caccttctgt ctgcggttgc cgggatcggg
     2341 ttccagcgtg accatgtcct tagccatcag ctccttgagg ccctggcgga tggcctgctt
     2401 gctcacgcga agtcgtcggg cgaggtctga cgggctatcc acgccctcgg caatgtagac
     2461 catcatcatg gactgggcgc gcgggagcga taggcccgcg tgctccttca tgtaggcctg
     2521 cagcccgtcg tccagccagt aggtggcgtg caccagctcg cgcaggaggg cgggtcccga
     2581 ggcgtgctcg agcggtgatg ccgtggatgt ctgatcgcgg ttggtcacgc gccggtgatc
     2641 ggctcgccgt tggccttgat ccctgcagcg tttaccggca gctccacttc cggggaaccg
     2701 gtgttcaggg tcgcgcagaa ctccagcatg atgccgtccg ggtcgtagaa gtagaacgag
     2761 cgaagccagg tcttcgcgct gatgcccgac ccgcgtcgag attcctctcc ggtgacgacg
     2821 tcgtcgtggt tgaccagggg cgtgcagttc acgccgcgcg aaaccagctt ctcccgataa
     2881 gcctcgatct gatccggagc cacgtcgaaa gcgacgtggt tcatggagcc gtgagccgag
     2941 gtgatcgagc cctctccgct tccgaccaga tgtgcggcgc tggcgacccc gggttgagcg
     3001 tcctttgcat cgcggaacca gaagaacgcc agctcgttgc cgccgcccat at
//
LOCUS       DBNHKV010000064         3266 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1568473, whole genome shotgun sequence.
ACCESSION   DBNHKV010000064 DBNHKV010000000
VERSION     DBNHKV010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1568473"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /gene="argA"
                     /locus_tag="AC16PP_01845"
     CDS             complement(<1..912)
                     /gene="argA"
                     /locus_tag="AC16PP_01845"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003641.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-CoA as donor [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HIR6480394.1"
                     /translation="MSDRPAKAEAENGYARWFRASTPYISAHRNRTFVILLGGDAVAH
                     GNLPNIVHDLALLHVLGARVVVVHGARPQIDAALGESPFHEDRRITDAAAMETIIGIL
                     GQQRARLEALFSTGLPTSPLHNTNISVVGGNFITARPVGIHDGVDHLFTGRPRRVHGG
                     RIQKALDTGALVLLSPLGYSPSGQAFNLAADELAADVAMALDADKLIAFGSDRYVSNE
                     RGEPQPNLTPAQLDTLIDVPALDDTTTRGHLRALLRACRGGVPRCHLVSYAHDGALLE
                     ELFTAGGGGTQISEDHSNLVRQARLEDV"
     gene            1231..3000
                     /gene="tfpB"
                     /locus_tag="AC16PP_01850"
     CDS             1231..3000
                     /gene="tfpB"
                     /locus_tag="AC16PP_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly ATPase TfpB"
                     /protein_id="HIR6480395.1"
                     /translation="MPLAQEQLIDLPVLLDGLVKEGYISQRQAEDILITPRTKKELSQ
                     HPMEIVADRDFDNRNKPGHKLDLETMTVWLAHQSGQPYLRIDPLDINVGAVTDVMSYA
                     FAQRHNILAVDVTDDEVVIASAQPYMYQWETMLTQTLRGRRIRRVVTNPADLSRYMLE
                     FYTMARSVAKAQDAGMEMSGLANFEQMLELGQMKSPEANDSHIVNIVDWLLQYAFDQR
                     ASDIHIEPRRDKGLIRFRIDGVLHHVYELPQQVNAAVTSRLKILGRMDVAEKRRPQDG
                     RVKTLSPDGDEIELRLSTLPTAFGEKMVLRIFDPDVLLRSFKELGLSGDDYERWHGMT
                     HSAHGIVLVTGPTGSGKTTTLYSTLKQLATSEVNVCTIEDPIEMVEPAFNQMQVQSNI
                     NVTFAAGVRALLRQDPDIIMIGEIRDLETAEMAIQAALTGHLVISTLHTNDAPTAITR
                     LLDLGVPPYMIKATVLGIMAQRLVRTLCPHCKVEEETDAEAWRLLTKPFRAKQPERVF
                     APKGCLECRDTGYMGRVGIYELLPMSEKVQTLVQADTDMDVLRKQAFKDGMLTLRLSG
                     AQKVGSGLTTISEVLRVAPASQD"
BASE COUNT          591 a         1041 c         1041 g          593 t
ORIGIN      
        1 cacgtcttcg agccgcgcct gccgcacgag gttcgagtga tcttcgctga tctgggtgcc
       61 gcctccgccg gccgtgaaga gctcctccag cagcgcgccg tcatgcgcgt agctcaccag
      121 gtggcaacgc ggcactccgc ctcgacaggc gcgcagaagc gctcgcagat gaccgcgggt
      181 ggtcgtgtca tccagggccg gaacgtcgat cagggtatcg agctgggcgg gtgtcaggtt
      241 gggttgcggc tcgccgcgtt cgttgctgac gtagcgatcg ctgccaaagg cgatcagctt
      301 gtcggcatcc agggccatgg ccacgtctgc cgcgagctcg tccgcggcca ggttgaacgc
      361 ctgcccggaa ggcgagtagc ccaggggaga caacagcacg agcgcgccgg tatccagggc
      421 tttctggatt cgccccccgt ggactcgtcg cggccggccg gtgaacaggt ggtccacgcc
      481 gtcatggatg ccgacgggtc gcgccgtgat gaagttgcca ccgaccacgg agatgttcgt
      541 gttgtgcagt gggctggtgg gcagcccggt ggagaagagg gcctccagtc gggcgcgctg
      601 ctgtcccagg atgccgatga tcgtctccat ggcggctgcg tcggtgatgc ggcgatcctc
      661 gtgaaagggc gattccccga gggccgcatc gatctgcggc cgtgcgccgt gcaccactac
      721 cacccgtgca cccaggacgt gcagcagcgc gagatcgtga acgatgttcg gcaggtttcc
      781 atgggcgacg gcgtcgcccc cgagaaggat gacgaacgtt cggttgcggt gcgcgctgat
      841 gtagggggtc gacgccctga accatcgtgc gtatccgttc tctgcttctg ccttggccgg
      901 tctgtcgctc atgaattgct gccctgccgc cgcccccctc gggaagcgcg tcgtcaaaaa
      961 agggggcgca ttttacacca gcgacctcga atggcatggc aaacgtgacg ccggtctaat
     1021 tccgaaaatg ccatcgccgg ctctgggcgc tagccggttg caaaagccgc atccttgtca
     1081 ttaactgaaa cttcttggct aatatcaggg acttgcgtgg ccaaccccct cattgattct
     1141 ccggcgggca gggatccgcg tccgctgtcg gagggagctg ctccgttggc agccgggtcg
     1201 gagccgaagg tcgcctccct tgccagccgc ctgccgttgg cgcaggagca gctcatcgac
     1261 ctgccggtgc ttctcgacgg gctcgtcaag gaaggctaca tcagccagcg tcaggcggaa
     1321 gatatcctga tcacgccgcg caccaagaaa gagctcagcc agcatcccat ggagatcgtt
     1381 gcggatcgcg atttcgacaa ccgtaacaag cctggccaca aactcgacct cgagaccatg
     1441 acggtctggc tggctcatca aagcggtcag ccctatctgc gcatagatcc gttggacatc
     1501 aatgtcggcg cggtcacgga cgtgatgtcg tacgcctttg cgcaacggca caacatcctc
     1561 gccgtggacg tgaccgacga cgaagtcgtc atagccagcg cccagcccta catgtaccag
     1621 tgggagacca tgctcaccca gaccctgcgg ggccggcgca tcagacgggt ggtgactaac
     1681 cccgcggact tgagccgcta catgctcgag ttctacacca tggcccgctc ggtggcgaaa
     1741 gctcaggatg cggggatgga gatgagcggc ctggcgaact tcgagcagat gctcgagctc
     1801 ggccagatga agtctccgga agccaatgat tcgcacatcg tcaacatcgt cgactggctt
     1861 cttcagtacg cgttcgacca gcgggccagc gacattcaca tcgagccgcg tcgcgacaag
     1921 ggactgatcc gttttcgcat cgacggcgtt ctgcaccacg tctacgagtt gccgcagcag
     1981 gtgaacgcgg cagtcaccag ccggttgaag attctgggcc gaatggacgt ggctgagaaa
     2041 cggcgccctc aggacggtcg cgtaaagacc ttgagcccgg atggcgacga gatcgagctg
     2101 cgcctgtcga ccctgcccac ggcgttcggc gaaaagatgg tgctccgtat ctttgatccg
     2161 gacgtgctgc tgcgcagctt caaggagctc gggctgtccg gcgacgatta cgagcggtgg
     2221 cacggcatga cccactctgc tcacggcatc gtcctggtca cgggcccgac cggctcgggg
     2281 aagacgacga ccctgtactc gacgttgaag cagctggcca cttccgaggt caacgtctgc
     2341 accatcgagg accccatcga gatggtcgag cccgccttca accagatgca ggtgcagtcg
     2401 aacatcaacg tcaccttcgc cgccggcgtg cgcgcccttc tccggcagga cccggacatc
     2461 atcatgatcg gtgagattcg tgatctcgag acggcagaga tggcgattca ggcggctctc
     2521 accggccacc tcgtcatatc cacccttcac accaacgacg ccccaaccgc gatcacccgt
     2581 ctgctcgatc tcggggttcc cccttacatg atcaaggcca ccgttctcgg tatcatggcc
     2641 cagcgtctgg tgcgtacgct ctgtcctcac tgcaaggtcg aagaagagac cgatgcggag
     2701 gcttggcgac ttctgaccaa gccgttccgc gcaaagcagc cggagcgggt gttcgcaccc
     2761 aaggggtgtc tggagtgccg ggacacgggg tacatgggac gggtgggcat ctatgagctg
     2821 ttgccgatga gcgagaaggt ccagaccctg gttcaggcag acacggatat ggatgttctg
     2881 cgcaaacagg ctttcaagga cggcatgttg acgctccgcc tgtcgggtgc gcagaaggtc
     2941 ggctctggcc tgaccacgat ttcggaggtc ctgcgggtgg ctccggcctc ccaagactga
     3001 acatttgtcc tcttcttcgg tacccccaga actgctgagc aaggccgagc gaacggctgc
     3061 ccgacttccg tatccccttc ctgccgacgg gctgcgggtg ctcgcgttga gcgacttcgc
     3121 cgccaacgtg atggcgcggg aggcccaatg gattcaggac gcgctcgagg ccgaccgctt
     3181 ctccgccccc ctcgcgtcgg gggaagtccg cgcgacgctc gccgccgttt cccaaggggc
     3241 cgaggaccta ggcgccctgc agcaga
//
LOCUS       DBNHKV010000065         2945 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1214476, whole genome shotgun sequence.
ACCESSION   DBNHKV010000065 DBNHKV010000000
VERSION     DBNHKV010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1214476"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="AC16PP_01855"
     CDS             complement(<1..348)
                     /locus_tag="AC16PP_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6480396.1"
                     /translation="MSIEPQRVRDYMSRELLTVSPETDIMRAIHLLVEHDISGLPVVD
                     AQDGLCGILTERDCIRIAVQAGYFDEAGGRVADYMSPSVHTVTPDDSLMDLAELFVES
                     PFRRCPVVEDGRLV"
     gene            complement(370..1593)
                     /gene="nqrF"
                     /locus_tag="AC16PP_01860"
     CDS             complement(370..1593)
                     /gene="nqrF"
                     /locus_tag="AC16PP_01860"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HIR6480397.1"
                     /translation="MLEIVVPAVALTTIVFLLSLAVLLVRRGLVPGEDVEILINDQRA
                     LRGDAGEKLLWALADHDVYLPAACGGRGSCGQCRIEVTRGGGAITPAEENHIARRDAA
                     RGVRLACMVTLGDDVAVHLPPELLEIRRWSSKVLANRHVTTYLKELTLELPPGLELNF
                     NAGAYVQLEAPAGIVRFDGFDIAAEYDAEWQRHGLRDLVVEIPETTTRAYSLASHPGE
                     SGAIKLVVRIALPPPDAPVGTPPGRVSSYAFGLKPGDEVWLAGPFGEFRARDTDREMV
                     LIGGGAGIAPLRAIVLDQLLNKRSGRKISFWYGARNERELCYHSELTELARQHENFAY
                     RVALSDPAPDSAWQGLTGLVHAVVYREYLEGHPSPEEAEYYLCGPPLMSSAVLAILED
                     LGVDRDSVFFDDFGS"
     gene            complement(1596..2204)
                     /gene="nqrE"
                     /locus_tag="AC16PP_01865"
     CDS             complement(1596..2204)
                     /gene="nqrE"
                     /locus_tag="AC16PP_01865"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HIR6480398.1"
                     /translation="MEHLLNIVVRAIFVENLALVFLLGMCTYIAVSKRVETAIGLGVA
                     VVVVQLITVPVNNLLYEYVLTPGALAWMGLGTLDLSFLKLITFIGVIAALVQMLELVI
                     ARFAPQLQQHLGIYLPLLTVNCAILGASLLMVQRSYDFAESVAYGLGVGIGWAMAIVL
                     FAAIRERLKYSDVPKGLEGLGLAFIVTGFLAFGFIGFTGVEF"
     gene            complement(2204..2854)
                     /locus_tag="AC16PP_01870"
     CDS             complement(2204..2854)
                     /locus_tag="AC16PP_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HIR6480399.1"
                     /translation="MLRHLTHPLVSENPITVQILGLCSALAVSSTLKPAFIMSVSVVA
                     VLVFSNVSVSLLRNVFPRSIRLILEVTLIASAVIVVDEVLKAFAPETADILTVFVGLI
                     VTNCIILGRAEAFAMHHRVAPSLADALGNGLGYALVLLAVATARELFGHGTLLDVQIL
                     PLSEAEGGWYRPNQMMRFAPSAFFLIGLIIWGVRSVRAEQVEAGAYPPASPSYREG"
BASE COUNT          594 a          953 c          958 g          440 t
ORIGIN      
        1 caccagccgg ccatcctcca ccaccgggca gcgtcgaaaa ggcgactcca caaaaagctc
       61 ggcgaggtcc atcaggctgt cgtcgggcgt gaccgtgtgg accgaagggg acatgtagtc
      121 cgccacccgg ccgcccgcct cgtcgaaata cccggcctga accgctatgc ggatgcaatc
      181 ccgctccgtc agtatcccgc agaggccgtc ctgcgcgtcc accaccggca gtcccgaaat
      241 gtcgtgctcg accagcagat gaatggcccg catgatgtcg gtctcgggcg agacggtcag
      301 aagctcccgc gacatgtagt cccttacccg ttgaggttcg atgctcatga tcatgcctcc
      361 tttgtccgat caggatccga agtcgtcgaa gaacacgctg tcgcggtcca caccgaggtc
      421 ttcgagaatg gccaggaccg cgctgctcat cagcggcggc ccgcagaggt agtactcggc
      481 ttcctcggga gacggatgcc cctccaggta ttccctgtac acgacggcgt gcacgaggcc
      541 ggtcagtccc tgccaggcgg agtccggcgc tggatccgac agcgccacgc ggtaggcaaa
      601 attctcgtgc tggcgggcga gctcggtcag ctccgagtgg tagcagagct cccgctcgtt
      661 gcgggcgccg taccagaaac tgatctttcg ccccgaccgc ttgttgagca gctggtcgag
      721 cacgatggcg cgcagcggcg cgatgcccgc gccgccgccg atgagaacca tttcccggtc
      781 ggtatcccgc gcgcgaaact cgccgaacgg gcccgccaac cagacttcgt caccgggctt
      841 caggccaaac gcgtaggacg agacccgccc gggcggggtc cccaccgggg catccggggg
      901 cggcagggcg atgcgaacga ccagcttgat ggcgccggat tcgccggggt ggcttgcaag
      961 agaatacgcg cgggtggtcg tctccggaat ttccacgacg agatcccgaa ggccgtgccg
     1021 ttgccattcg gcgtcgtact cggcggcgat gtcgaagccg tcgaaccgca cgatgccggc
     1081 cggggcctcg agctgcacgt aggcgccggc gttgaaattc aactccaggc caggcggcag
     1141 ctcgagggtg agctccttca agtaggtggt cacgtgacgg tttgccagaa ccttcgagct
     1201 ccagcggcgg atctccagca actcgggagg cagatgcacg gcgacgtcgt cgccaagggt
     1261 gaccatgcag gcgaggcgca cgccgcgagc ggcatcacgg cgcgcaatgt ggttctcttc
     1321 cgccggcgtg atggcgccac cgccccgcgt cacctcgatg cgacattgcc cgcagctgcc
     1381 ccggccaccg caggccgccg gaagatagac atcgtggtcg gccagcgccc acaggagctt
     1441 ctcgccggcg tcgccccgca gagcccgctg atcgttgatc agaatctcca cgtcctcgcc
     1501 gggcaccagg ccgcggcgga cgaggagcac cgccagcgag agcaggaaga cgatggtcgt
     1561 cagcgcgacg gctggcacga cgatctccag cacgtctaga actccactcc ggtgaagccg
     1621 atgaagccga aggcgaggaa cccggtcacg atgaaggcca ggccaaggcc ttcgagcccc
     1681 ttcggcacat cgctgtactt caagcgctct cggatggcgg caaagagaac gatggccatc
     1741 gcccagccga tgccgacgcc gagcccgtaa gcgacgcttt cggcgaagtc atagctgcgc
     1801 tgcaccatga gcagggaagc gccgagtatg gcgcagttga cggtgagcag cggcaggtag
     1861 atgcccaggt gctgctgcag ctgcggcgcg aaccgcgcga tgacgagctc cagcatctgg
     1921 acaagagcgg cgatgacgcc gatgaaggtg atcagcttga ggaacgaaag atccagggtt
     1981 cccagcccca tccaggccag cgccccggga gtgaggacgt attcgtagag gaggttgttg
     2041 acgggcacgg tgatgagctg cacgacgacg acggcgacgc cgagcccgat ggcggtctcc
     2101 acacgcttgg agaccgcgat gtacgtgcac atgccgagca ggaacaccaa agccaggttc
     2161 tcgacgaaga tggcgcgcac aacgatgttc aggagatgct ccatcagccc tcccggtagc
     2221 tcgggctcgc cggcgggtaa gctccggctt cgacctgttc cgcgcgcacg ctgcgaacgc
     2281 cccagatgat gagcccgatc agaaaaaaag cgctgggcgc aaagcgcatc atctggttgg
     2341 ggcgatacca accaccttcc gcctcgctca ggggcagtat ctgaacgtcg agcaacgtcc
     2401 cgtgcccgaa cagctcgcga gccgtggcga cggccagcaa caccagcgcg tagcccagac
     2461 cattcccaag ggcgtcggca agactggggg ccacccggtg gtgcatggca aacgcttcgg
     2521 cccgtccgag aatgatgcag ttggtgacaa tcaggccaac gaacacggtc agtatgtcag
     2581 ccgtctccgg agcgaaggcc ttgagcacct cgtccaccac gatcaccgcc gaggcgatga
     2641 gcgtcacttc caggatcaga cgaatgctgc ggggaaagac gtttctcaac aggctgacgg
     2701 acacattcga gaaaaccagc accgcgacaa cggaaacgct catgatgaaa gccggcttga
     2761 gggtgctgga caccgcaagg gcggagcaga gaccgaggat ctgcacggtg atcgggttct
     2821 cgctgacgag cggatgggtg agatgcctca acatgaacgg tgtccacgct tcatattcgt
     2881 gctccaggcg ccgcgatccg ggaaagccag ggcgagtaga tggccaacca gccggcgacc
     2941 agctg
//
LOCUS       DBNHKV010000066         2802 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1640295, whole genome shotgun sequence.
ACCESSION   DBNHKV010000066 DBNHKV010000000
VERSION     DBNHKV010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1640295"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1427
                     /locus_tag="AC16PP_01875"
     CDS             87..1427
                     /locus_tag="AC16PP_01875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6480400.1"
                     /translation="MSGPVLTSSFGLRMSAFTALYLAQGVPIGLFSIALPAWLVEQGA
                     DAADIAALVAVTGLPWAFKLIAGPFMDRFSFPAMGRRRPWVMGAQAGFTVSMVSLALV
                     SGPSDGLWPIMVIGFVINTFAAVQDVAVDGMAIDVLPISERGRANAFMAFGQAAGFSG
                     FGALDGYLLSRFGLGVTAIVSAAAIGAVLVFVAVIREREGERLLPWTAGMATAREIAP
                     ARSMLEILRDILRVLLLPMSLLLIAVEFLARAGSGVGLAIMPVLAVDELGYASEVYAY
                     WLGLMGGLSAFVGIFCGPAIDRLGARKVLVVGLLGGSLVTVAYALAAGARGTAGFDLT
                     MLAAAQIFGQVFFVSMIAMFMGICWARVAATQFAIYMSLANLARSAGAGAFALVATDL
                     SVFDALYLVAALSVAAVLLLLRFDPAAHAARLALIDEERLAPRQTERESRTPTP"
     gene            complement(1363..2127)
                     /locus_tag="AC16PP_01880"
     CDS             complement(1363..2127)
                     /locus_tag="AC16PP_01880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR6480401.1"
                     /translation="MLTFLALTAIIAFRYMLISGLFYWLLWRRKSSGAGAIKLMAGQP
                     AAGAVQKEIGWSLLSSFIYAAPAAVVFELWRLGGTAVYMDLAAMPLWYLPVSVGVYLF
                     LHDTYFYWTHRLMHQKRIFPIMHRVHHESRPPTPWASFSFHPYESVLGAIFLPALALV
                     IPIHIGAVLFILTVMTLAAVLNHSGYEIFPRWWLRGWPGHHLITAAHHDLHHKHFSCN
                     YGLYFRFWDKLMRTDIMESEYDFLAPSAEVPASPHR"
     gene            2220..>2802
                     /gene="crtY"
                     /locus_tag="AC16PP_01885"
     CDS             2220..>2802
                     /gene="crtY"
                     /locus_tag="AC16PP_01885"
                     /EC_number="5.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045436 - lycopene beta cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene beta-cyclase CrtY"
                     /protein_id="HIR6480402.1"
                     /translation="MHYDIIFVGGGLAGSLCAYRLRQLRPELGVLVLEQEARLGGNHT
                     WSFFASDLDEAELGWTEPFVAHQWDGYEVRFPGRRRRLTTGYRSSNSDRLHAVVSVAL
                     GDGVRCGAAVAAMDAGSVRLRDGETLTARCVVDARGPDRAPRLELGFQKFTGLILDLA
                     APHGLEVPIIMDATVDQGGDYRFVYVLPLGPSEV"
BASE COUNT          454 a          826 c          951 g          571 t
ORIGIN      
        1 ctcatcttgt cttaccaggg aatcagcata ttggaagacg cgggtcagga aaataagcgt
       61 cgtcacgatc gtgggagcgt gtccctgtga gcgggccggt tctcacctcg tccttcgggc
      121 tgcgcatgtc ggcattcacc gccctttacc tggcgcaggg cgtacccatc ggtctgttct
      181 cgatcgctct tccggcatgg ctcgtggagc agggcgcgga tgctgcggac atcgctgcgc
      241 tcgttgccgt caccgggctt ccctgggcct tcaagctgat cgccgggccc ttcatggacc
      301 gcttcagttt ccccgccatg ggtcggcgtc gaccctgggt catgggtgcc caggccggct
      361 ttaccgtatc catggtctcc ctggccctgg tgtccggccc ttcggatggc ctctggccga
      421 tcatggtgat cggcttcgtg atcaacacgt tcgccgccgt tcaggacgtg gccgtcgacg
      481 gcatggccat cgacgtgctg ccgatcagcg agcggggccg ggcgaacgct ttcatggcgt
      541 tcggccaggc agcgggcttc tcgggattcg gtgcgctgga cggctacctg ctcagccgtt
      601 ttggtctggg cgtcaccgcc atcgtatcgg ctgcagccat cggcgcggtc ttagtcttcg
      661 tcgcggtgat acgggagcgg gaaggggagc gtctgctgcc ctggacggcg ggcatggcga
      721 ctgcccggga gatcgccccg gcccgatcga tgctggaaat tctcagggac attctccggg
      781 tgctcctgct gcccatgagc ctgcttctca tcgccgtgga gtttcttgcg cgcgcgggtt
      841 cgggagtcgg gttggccatc atgcccgtgc tggccgtaga cgagctgggt tacgcatcgg
      901 aagtttacgc ctactggctt ggattgatgg gcgggctgag cgccttcgtc ggcatattct
      961 gcgggcccgc catcgatcgc ctgggcgccc ggaaggtgct cgtggtgggc ctgctcggcg
     1021 gctcgctggt aacggttgct tatgcgctgg cggcgggggc gaggggtaca gccggattcg
     1081 atctcaccat gctggccgct gcccagatat tcggtcaggt cttcttcgtg tcgatgatcg
     1141 ccatgttcat gggcatctgc tgggcaagag tcgccgctac ccagtttgcc atctacatgt
     1201 cgctggcgaa cctggccagg tcggctggcg caggcgcgtt tgcgctcgtc gcgacggatc
     1261 tcagcgtatt cgatgcgctc tacctggtcg cagcgctgag cgtcgccgcg gttttgctgc
     1321 tcctgcgatt cgatcctgcg gcccacgccg cccggctggc gctcatcgat gaggagaggc
     1381 tggcacctcg gcagacggag cgagaaagtc gtactccgac tccatgatgt ccgttctcat
     1441 cagcttgtcc cagaaccgga agtagagacc atagttgcag ctgaagtgct tgtgatgaag
     1501 gtcatggtgg gcagccgtga tcaggtggtg cccgggccaa ccacgcagcc accatcgggg
     1561 aaagatctcg tatcccgaat gattcagcac ggcagcgagt gtcatcacgg tcaatatgaa
     1621 gagaacggca ccgatgtgaa tgggaatcac cagagcgagc gcgggcagaa agatggcgcc
     1681 gagaacggac tcgtaggggt gaaacgagaa tgacgcccag ggcgtgggcg gcctggactc
     1741 gtgatggacc cggtgcatga tcggaaaaat ccgcttttgg tgcatgagac gatgggtcca
     1801 gtagaaatag gtatcgtgca gaaagagata gacccccacg ctcacgggca gataccaaag
     1861 cggcatggcg gccaggtcca tgtagacggc ggtgccgccg aggcgccaca gctcgaagac
     1921 cacggccgct ggtgcagcgt agatgaacga ggacagcagt gaccagccga tttccttttg
     1981 caccgcgccg gcagctggct ggccggccat gagcttgatc gcccctgcgc ccgatgactt
     2041 tcggcgccac agcagccagt agaacagacc ggagatcagc atgtacctga acgcgatgat
     2101 cgcggtcagc gcgaggaacg tgagcaacag cctttccggg ggaacgtcgg ccagcatggg
     2161 ttgggcggag tgaggagctt cttcgctaga atgtcggcag tttagcgggg actcgccgat
     2221 tgcactacga catcatattc gtcgggggtg ggctcgccgg tagcctatgt gcttaccggc
     2281 ttcgtcagct gcgtccggag ctcggcgtgc tcgtcctgga gcaggaagcg cgcctcggcg
     2341 gtaaccacac ctggtcgttc ttcgcttcgg atctcgacga agccgagctt ggctggacgg
     2401 aacctttcgt ggcgcatcag tgggacggct acgaggtccg ctttccggga cgccgccgca
     2461 ggctcacgac cggataccgg tcgtccaact cggatcggct ccatgccgtg gtgtctgtgg
     2521 cgctcggcga tggggttcgc tgcggggcgg cggtggcggc aatggatgcc ggttcggtca
     2581 ggctccggga tggagaaacc ctcacggccc ggtgcgttgt cgatgctcgt ggcccggaca
     2641 gggcaccgcg cctggaactc gggtttcaga aattcaccgg gttgattctg gatctcgccg
     2701 cgccccatgg tctggaggtg cccatcatca tggatgcaac ggttgatcaa ggaggcgact
     2761 accggttcgt ttacgtcctt cccctgggtc cgtcggaggt gc
//
LOCUS       DBNHKV010000067         7844 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_789908, whole genome shotgun sequence.
ACCESSION   DBNHKV010000067 DBNHKV010000000
VERSION     DBNHKV010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7844)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7844)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7844
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_789908"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..909
                     /locus_tag="AC16PP_01890"
     CDS             211..909
                     /locus_tag="AC16PP_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6480403.1"
                     /translation="MSRPEGSILLVEDNADLAETVGAFLEAKNYVVDYAADGLLAVQL
                     ATSEAFDAIVLDIMLPGIDGIEVCRRLRRDARLSTPIIMLTARDQLQDKLDGFDVGAD
                     DYLVKPFDMPELVARIEALIRRGRGVSAAYNVGELSMDLDTMSVFRGDTEILLSRTLF
                     DILKILLREHPKVVPRETIERELWGDDLPDSDTLRSHLYNLRKAVDRPFNEPLIETLA
                     GRGYRVRVPEPDAG"
     gene            989..1939
                     /gene="folE2"
                     /locus_tag="AC16PP_01895"
     CDS             989..1939
                     /gene="folE2"
                     /locus_tag="AC16PP_01895"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HIR6480404.1"
                     /translation="MSTPKPVELASMPDIAGRPHAKPQGTLDWVGMSDIHQPILIRAG
                     NVNQRVQARVQVYVDLNDPHAKGIHMSRLYLLLDEHARTRPLSVAGLKMLLGSMHSSH
                     LDLSSRAFVQFDFEYELRQMALLSDNAGWSSYPITVKGTLADGKMTLELGVEILYSST
                     CPCSAALARQLIQQAFADRFDSGRPIDFADVNEWLGTEQGIVATPHSQRSVAKVITQL
                     ADDVTELPIAALIDTLETAIGTPVQAAVKRQDEQEFALRNGQNLMFCEDAARKLQAAL
                     NDDPSFNDFWLRVEHHESLHPHDAVSIVTKGIDGGYLPIP"
     gene            complement(1941..3236)
                     /locus_tag="AC16PP_01900"
     CDS             complement(1941..3236)
                     /locus_tag="AC16PP_01900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6480405.1"
                     /translation="MKPKGIRSKLVRAFFIQVTIISCVTLVGIAVAYNIIYGVMLREA
                     LNDEAAHFWSRYESDPSHSLPDVYNLQGFMAVDGDFSGVPEQYRGLAPVGFSRQPYED
                     AQALVHVSERDGERLFLVFEYDQVSALAFFFGIVPLALVLLLIYGTSFVTYRLSQRAV
                     SPIVRLAAYLEDFDFNANLEISRDLEPLRAHADAEVVSMIQAVEHFTTRLAAFVERER
                     IFTRDAGHELRTPVAIFKGSLDLLEQSGDRPAHEHKALARMRRTVSDMESLLETLLLL
                     AREEQVEMPREDVSVNALVAGQLELLQPMAERAENSLALHERAELRVRAPEKVLEIVL
                     GNLIRNAINYTHGGRVDVTVTDRSVQVGDTGVGMSGEELQNAFEPFYRVDESRGAIRG
                     HGLGLAIVKRLAHQAGWSISAHSKPGEGTRVEVAFFPDD"
     gene            complement(3273..4304)
                     /locus_tag="AC16PP_01905"
     CDS             complement(3273..4304)
                     /locus_tag="AC16PP_01905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HIR6480406.1"
                     /translation="MSILIDLAEARRFPDALVRAGIRRLVAQRLKDEGSQGVAAQAER
                     HQRLLRELQESPVALDTELANEQHYEVPAEFFRAVLGKHLKYSSCYWERGAADLDEAE
                     AHMLALYGERAELADGQRVLDLGCGWGSFTLWAAERFPGSRFTAVSNSNGQRAYIESQ
                     AAARGLKNVEVITADVNDLRLPGIYDRIVSIEMFEHMRNYALLLNRIAGWLASDGKLF
                     VHIFCHRHLLYPYETEGDGNWMGRYFFTSGLMPAADTLLFFQDDLRCERRWSVSGVNY
                     EKTARAWLDNLDANESRVVQALEGTYGAAEVARWVQRWRMFFMACEELFAYRGGEEWL
                     VCHYRFAPR"
     gene            complement(4338..5591)
                     /locus_tag="AC16PP_01910"
     CDS             complement(4338..5591)
                     /locus_tag="AC16PP_01910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6480407.1"
                     /translation="MTDNTLYGDGRRTAPSGPFAALARKALGRLRDGTIRLTEAGRTE
                     VFGCGAPEVHVEVRDPRFFRRLVMGGSVGAGESYMDADWSCDDLVSLIRIFARNRDAL
                     DVVDGAGSVLVRWANRSRHLLRRNTRRGSRRNIEAHYDLSNEFFETFLDGRMMYSSAV
                     FESPDATLEEASEAKLERLCRKLELGPEDHLLEIGSGWGGLAVYAAAQFGCRVTTVTI
                     SPAQLEATQARVRAAGLQGRVRAVLSDYRDLDGCYDKVVSVEMVEAVGHEFLDRYFES
                     LSGLTRPGGLIALQAITIADRRYRAALAEADFIKKHIFPGSFIPSVCVLVDAAARRTD
                     AVLVNLEDIGLDYARTLRAWRERLEARRGEIQSLGFDERFLRMWRFYLAYCEGGFLER
                     AISDVQLLFAKPAYRGQPWRGALDG"
     gene            complement(5588..6346)
                     /locus_tag="AC16PP_01915"
     CDS             complement(5588..6346)
                     /locus_tag="AC16PP_01915"
                     /inference="COORDINATES: protein motif:HMM:NF018770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HIR6480408.1"
                     /translation="MPPELLRDSVCEGWVYHRRAAPEHRFRYRVWMLWLDVARLGQGP
                     LRLLRRRLGLSPLGIAPKDFLDGSDDLLGAVRARLASHGYPAPARVFLLTQPRSWISG
                     FNPVSFYFCVGQAGLDCILAEINNTPWDERHTYVLDARGQSGEFSFSFPKAFHVSPFM
                     PMDLEYRWRFAIEPEAVKIAMGLFREGEETFNAGLRLSVVPMTRRRLLLGMARYPLQN
                     VATLARIYWQALRLYSKRSRFYPHPDRAEEAPAS"
     gene            complement(6336..7577)
                     /locus_tag="AC16PP_01920"
     CDS             complement(6336..7577)
                     /locus_tag="AC16PP_01920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6480409.1"
                     /translation="MRIAVVGAGVSGIAAAYRLQQEADVVLFEGQGRLGGHTDTHAVL
                     VGGATHAVDSGFIVFNPDNYPRFSSWLDELGVESQPSDMSFGVSNQATGVEYGSRGLG
                     ALFCQRRNLVSPRFLRMLADLRRFYRDAVDYPLDESLTLGELLERHGYGAGFVEDHIG
                     PMCGALWSAAPDAALAIPARHVIAFMTHHRMLQLDGRPQWRVIKGGSSTYLQAFLARF
                     SGEVRLADPVQAVARTASGVDITSASGTARFDAVVLACHSDEALGLLADPSEAERDVL
                     GAIGYQPNRVVVHSDDRVMPEHRPAWSSWNALVGAERPERCQVSYWMNLLQSLPGREN
                     FFVTLNPQMPLKRVWSVREYAHPVFTRRARAAQRRKAEISGVRNTWFCGAYWGWGFHE
                     DGFVSGMEVAEGMLGSMADAA"
     gene            complement(7574..>7844)
                     /locus_tag="AC16PP_01925"
     CDS             complement(7574..>7844)
                     /locus_tag="AC16PP_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HIR6480410.1"
                     /translation="TLLVNSLAHRSGRRCFDTRDESRNNWIVAVLTLGEGWHNNHHRF
                     AGAARQGFYWWQLDVSFYLLKLMSWLGLIWDLKPVPGSVLREGRS"
BASE COUNT         1458 a         2662 c         2468 g         1256 t
ORIGIN      
        1 gccgcggtct tccgctcagc gaacgcttgc aatgcgccac agggcgacta ttatctcgcc
       61 tccctttccc cgcccgcggg ccgatgccgg ggcgacaggg tgcgcgggcg tagctcaggg
      121 ggacggatca ttgtgcccat tgccgcgagc cgaaaccaag gggcttcgag ggcgttgcgg
      181 ccggcgtgac cctgcctcta agatgcgccg atgagtcgac ccgaaggcag tatcctcctc
      241 gtggaagaca acgcggacct ggcagagacg gtcggcgcgt tcctggaggc aaagaactac
      301 gtggtggact acgccgcgga cgggctgctc gccgtgcagc tcgcgaccag cgaagccttc
      361 gacgccatcg tgctggacat catgctgccc ggcatcgacg gcattgaggt ctgccggcgc
      421 ctgcgcaggg atgccaggct ctccaccccc atcatcatgc tcaccgcccg ggaccagctt
      481 caggacaagc tcgacggctt cgacgtcggc gcggacgact atctggtcaa acccttcgac
      541 atgccggagc tggtcgcgcg catcgaggcg ctcatccgcc gcggccgcgg ggtgagcgca
      601 gcctacaacg tgggcgagct gagcatggat ctggacacca tgtccgtgtt ccggggcgac
      661 accgagatcc tcctatcgcg tacgctgttc gacatcctca agatcctgct gcgcgagcac
      721 ccgaaggtgg tcccacgcga gaccatcgag cgggaattgt ggggagacga ccttcccgac
      781 agcgacaccc tgcgcagcca cctctacaac ctgcgcaagg ccgtcgaccg gccgttcaac
      841 gagccgctca tcgagactct ggcaggccgc ggataccgcg tgcgggttcc tgagccagac
      901 gccggctgac gggcaacgcc tttcacaccc ctttcaccgg cgcccctcta gcttgatctc
      961 atcagatcat tgatcgaggg gataacccat gagcacacca aagcccgtcg agctggcctc
     1021 catgcccgac atcgccggac gaccccatgc caagccgcaa ggcaccctgg actgggtggg
     1081 aatgagcgac atccatcagc ccatcctgat ccgggccggc aacgtcaacc aacgcgttca
     1141 ggccagggtc caggtgtacg tggacctgaa cgacccccat gccaagggca tccacatgtc
     1201 ccggctgtat cttctgctcg acgagcacgc ccgcacccgt cccctgtccg tcgcggggct
     1261 gaaaatgctg ctcggctcca tgcacagctc tcatctcgac ctgagcagcc gggcgttcgt
     1321 gcagttcgac ttcgagtacg agctgcgcca gatggcgctg ctttcggaca atgccggctg
     1381 gagctcctac cccatcaccg tgaagggcac cctggccgac ggcaagatga ccctggagct
     1441 gggggtggag attctctact cgagcacctg cccttgctcg gccgcgctgg ccaggcagct
     1501 gatccagcag gcgtttgccg accgcttcga cagcggccgg cccatcgact tcgccgacgt
     1561 caacgagtgg ctcggcacgg agcagggcat cgtcgccacg cctcacagcc agaggagcgt
     1621 ggcaaaggtc atcacccagc tggccgacga cgtgaccgag ctccccatcg cggccctcat
     1681 cgacacgctg gagaccgcca tcggcacgcc ggttcaggcc gccgtgaaac gccaggacga
     1741 gcaggaattc gccctgcgca acggtcagaa tctgatgttc tgcgaagatg ccgcgcgcaa
     1801 gctgcaggcc gcgctcaacg acgatccgtc cttcaatgac ttctggctgc gcgtcgagca
     1861 ccacgaaagc cttcaccccc acgacgcggt gagcatcgtc accaaaggca tcgacggcgg
     1921 ctacctgccc atcccctgac ctagtcgtcg gggaagaagg ccacttctac ccgggtccct
     1981 tcaccaggct tgctgtgggc cgagatgctc cagccggcct ggtgggccaa ccgcttcacg
     2041 atggctaggc ccaggccgtg gcctcggatc gcccctctgc tctcgtccac ccggtagaac
     2101 ggctcgaacg cgttctgcaa ctcctcaccc gacatcccga cgcccgtgtc gcccacctgg
     2161 acggagcggt cggttaccgt cacatcgacg cggccgccgt gggtgtagtt gatggcgttt
     2221 cggatcaggt tgccaagcac gatctcgagc actttctccg gggcgcgcac gcgcagctca
     2281 gcgcgctcgt gcagcgccag gctgttctcg gcgcgctcgg ccatgggctg cagcagctcg
     2341 agctggcccg ccaccagggc gttgacgctc acgtcctccc gaggcatctc tacctgctcc
     2401 tctctggcca gcagcagcag ggtctccagc agcgattcca tgtccgagac cgtgcgtctc
     2461 attcgggcaa gcgccttgtg ctcgtgagcc ggtcgatccc cgctctgctc gagcagatcc
     2521 aggctgcctt tgaatatcgc caccggcgtg cgcagctcgt ggccggcgtc gcgggtaaaa
     2581 atccgctcac gttcgacgaa ggctgccagc cgggtggtga aatgctccac cgcctgaatc
     2641 atgctcacga cctccgcgtc ggcatgggcg cgcagcggtt cgaggtcgcg actgatctcg
     2701 agattggcat tgaagtcgaa gtcctccaga taggccgcca ggcgcacgat gggtgacacc
     2761 gcccgctggg agagtcggta ggtgacgaag gacgtgccgt aaatcagcaa caggaccaac
     2821 gcgagcggga cgatgccgaa gaaaaaggcc agggcggaca cctgatcgta ctcgaacacc
     2881 agaaagagcc gctcgccgtc acgttccgat acgtgcacca gtgcctgggc gtcctcgtat
     2941 ggctgccggc tgaagcccac cggagcgagg ccccggtatt gctccggcac gcccgagaag
     3001 tccccgtcca cggccatgaa accctgcagg ttgtatacgt ccggcaggct gtgactcgga
     3061 tccgattcgt aacggctcca gaagtgcgcg gcctcgtcgt tgagggcttc ccggagcatg
     3121 acgccgtaga tgatgttgta cgccacggct atgcccacca gggtgacgca gctgatgatc
     3181 gtcacctgaa tgaagaaggc ccggacgagc ttggatctga ttcccttggg tttcatttca
     3241 acccgttctc cggcgggcgc agcgccgccc cgtcagcgtg gggcaaaacg gtaatggcac
     3301 acgagccact cctcgccgcc acgataggca aagagctcct cgcaggccat gaaaaacatg
     3361 cgccagcgct gaacccatcg ggcgacctcg gcagccccgt aggttccttc gagagcctgc
     3421 actactcggc tttcgttggc atcgagattg tccagccagg cgcgggccgt tttctcgtag
     3481 ttcactccgg aaacgctcca ccgccgctcg caccgcagat cgtcctgaaa gaacagcagc
     3541 gtgtccgctg ccggcatgag cccgctggta aaaaagtaac ggcccatcca gttgccgtcc
     3601 ccctcggtct cgtagggata aaggaggtgg cggtggcaga agatgtggac gaacagcttt
     3661 ccgtcggacg caagccagcc ggcgatacga ttcagcagca acgcgtagtt gcgcatgtgc
     3721 tcgaacatct cgatggatac gatacggtcg tagatcccgg gcagccgcag atcgttgacg
     3781 tcggccgtga tgacctcgac gtttttcagc ccgcgagcgg ccgcctgtga ctcgatgtag
     3841 gcgcgctggc catttgaatt gctgacggct gtgaagcggc tccccggaaa acgctcggcg
     3901 gcccagagcg tgaaggaacc ccagccgcag cccagatcga gaaccctctg gccgtcggca
     3961 agctccgccc gctcgccgta gagcgccagc atgtgcgcct ccgcctcgtc gaggtcggca
     4021 gcgccgcgtt cccagtaaca gctgctgtac ttgaggtgct ttccaagcac cgcacgaaag
     4081 aactcggcgg ggacctcgta gtgctgctcg ttggccagct ccgtatccag cgcaacgggg
     4141 ctttcctgga gctcgcgcag caggcgctgg tgtcgctccg cctgagcggc gacgccttgc
     4201 gacccctcgt ctttcagccg ctgcgcgacg agcctgcgga tacccgcgcg aaccagggcg
     4261 tccggaaatc ggcgcgcctc ggccagatcg atgagtatgc tcatgaaacc gtctccttgt
     4321 tgattcgagc cgccccctca accgtcgagg gcaccccgcc agggctgacc ccggtacgcg
     4381 ggctttgcga aaagaagctg tacgtcgctg atggcccgct ccaggaaccc gccttcgcaa
     4441 taagcgaggt aaaaccgcca catgcgcaga aaccgctcgt cgaagcccag cgactgaatc
     4501 tccccgcgcc gggcttccag gcgctcacgc caggcgcgca gggtgcgcgc gtagtcgagg
     4561 ccgatgtcct cgaggttgac cagcaccgcg tcggtccggc gcgccgccgc gtccaccagc
     4621 acgcagaccg agggaatgaa gctgccgggg aagatgtgtt tcttgatgaa gtctgcttct
     4681 gcgagcgctg cgcggtaccg ccggtctgca atggtgattg cctgcagggc gatgagccca
     4741 cctggtcggg tgagtccgga cagcgactcg aagtagcgat cgaggaactc gtgcccaacc
     4801 gcctctacca tctcaacgga taccaccttg tcgtaacagc cgtccagatc gcgatagtcc
     4861 gacagaacgg ccctgacgcg gccctggagc ccggcggccc gaacccgcgc ttgggtggct
     4921 tcgagctgag cgggggaaat cgtgaccgtg gtgacccgac agccgaactg cgctgccgcg
     4981 tacacggcga gcccgcccca gcccgagccg atctccagaa ggtgatcctc cgggccgagc
     5041 tcgagttttc ggcacagccg ctccagcttg gcttcgctcg cctcctccag ggttgcatcc
     5101 ggcgactcga acacggccga cgagtacatc atcctgccgt cgagaaaagt ctcgaagaac
     5161 tcgttgctca ggtcatagtg cgcttcgatg ttgcgccggc ttccccgtcg ggtgttgcgc
     5221 ctcagcaggt gcctggagcg gttggcccat cgcacaagaa cgctgccggc gccatccacc
     5281 acatccaggg cgtcgcggtt gcgggcaaag attctgatca gagacaccag atcgtcgcag
     5341 gaccagtccg cgtccatata tgactcgccc gccccgacgc ttccgcccat gaccaggcgc
     5401 cggaagaaac gcggatcacg gacctccacg tgcacctccg gggcgccgca accgaaaacc
     5461 tcggtacggc ccgcctcggt cagccggatg gtgccatctc gcaacctgcc cagcgccttg
     5521 cgagccagcg cggcaaacgg gcccgagggc gcggttcgtc ggccgtcgcc atacaaggtg
     5581 ttgtcggtca tgatgccggc gcctcctcag cgcgatcggg gtgcggatag aaacggctgc
     5641 gcttggaata gagccgaagg gcctgccagt agattcgtgc cagcgtcgcc acgttctgca
     5701 gcggatagcg ggccatgccc aggagcaggc ggcgccgggt catgggcact accgacagcc
     5761 tcagcccggc gttgaaggtc tcttcccctt ccctgaacag acccatggcg atcttcaccg
     5821 cttcgggctc gatggcgaac cgccaacggt attcgagatc catcggcatg aaaggcgaga
     5881 cgtgaaaggc cttgggaaaa gagaaggaga actcgcccga ctggccgcgc gcgtccagca
     5941 cgtaggtatg gcgttcatcc cagggcgtgt tgttgatctc ggcaagaatg caatcaagcc
     6001 ctgcttggcc cacgcagaag tagaagctga ccggattgaa cccggaaatc cagctgcggg
     6061 gctgggtgag cagaaacacc cgggccggcg ccgggtagcc atgactggcc aggcgcgcgc
     6121 gaaccgcgcc gagaaggtcg tcgctgccgt cgagaaagtc cttgggcgct atcccgagcg
     6181 ggctcagtcc cagccggcgc cgcagcaggc gcaagggccc ctggccgagg cgggcaacat
     6241 cgagccagag catccagacg cggtaccgga aacggtgttc cggggcggcg cggcggtggt
     6301 aaacccaccc ctcacagacg ctatcgcgga ggagctcagg cggcatcggc catagaccca
     6361 agcatgcctt cggcaacctc catgccgctg acgaagccgt cctcgtgaaa gccccagccc
     6421 cagtaagcac cgcagaacca ggtattgcga acgccgctga tctcggcctt gcgacgctgg
     6481 gctgcacggg cacggcgagt gaataccggg tgagcgtact cgcgcacgct ccacacgcgc
     6541 ttgaggggca tctgcgggtt caacgtcaca aaaaagtttt cgcgcccggg caatgactgg
     6601 agcaggttca tccagtagct cacctggcaa cgctcggggc gctcggcccc gaccaaggcg
     6661 ttccagctcg accacgccgg ccgatgctcg ggcatgaccc ggtcgtcgga gtgaacgacg
     6721 acccggttcg gctggtagcc gatggcgccc agcacgtccc gctccgcctc gctggggtcg
     6781 gccagcagac ccagggcctc gtcgctgtgg caggccagca cgacggcatc gaagcgcgcg
     6841 gttcccgacg ccgaggtgat gtccacgccc gacgccgtcc tggctaccgc ctgcaccggg
     6901 tcggccagcc gcacctcgcc gctgaaacgg gccagaaacg cctgcagata ggtggacgac
     6961 ccgcccttga tcacccgcca ctgcgggcga ccgtccagct gcagcatgcg gtgatgcgtc
     7021 atgaaggcga tcacgtggcg ggccggtatc gccagagccg cgtccggcgc agcggaccag
     7081 agcgcaccgc acatgggccc gatgtgatcc tcgacgaacc cggcgccgta gccgtgacgt
     7141 tcgagcagct cgccgagggt gagcgactcg tccaggggat aatcgacggc gtcacggtag
     7201 aacctgcgaa gatcggccag catgcgcagg aaccggggtg agaccaggtt gcgccgctga
     7261 cagaacaaag cgcccagtcc gcggctgccg tactcgacgc cggtagcctg gttcgagacg
     7321 ccgaaggaca tgtccgacgg ctgagattca acccccagct cgtccagcca cgacgagaaa
     7381 cgcgggtaat tgtccgggtt gaacacgatg aatccggagt ccacggcgtg ggtggcaccg
     7441 ccgacgagca cggcgtgggt gtcggtgtga ccgcccagcc gcccctgccc ctcgaagagg
     7501 acgacgtcgg cctcctgctg cagccgatag gcggcggcga taccggatac gcccgctccc
     7561 accaccgcga ttctcacgag cgcccctccc ggagcaccga gccagggacc ggcttgagat
     7621 cccagatcaa gccgagccag gacatgagct tcagcaggta gaaagagacg tcgagctgcc
     7681 accagtagaa tccctgtcgg gcagcgccgg caaagcgatg atgattgttg tgccagccct
     7741 cgccgagggt cagaacggcg acgatccagt tgttgcggct ttcgtcccgc gtgtcgaagc
     7801 acctgcggcc cgagcggtgc gccagggagt tcaccagcag ggtc
//
LOCUS       DBNHKV010000068         2217 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_82027, whole genome shotgun sequence.
ACCESSION   DBNHKV010000068 DBNHKV010000000
VERSION     DBNHKV010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_82027"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="AC16PP_01930"
     CDS             complement(<1..1132)
                     /locus_tag="AC16PP_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HIR6480411.1"
                     /translation="MTPRQILQQTFGYPAFRPPQDAIIDTVMAGRDALVLMPTGGGKS
                     LCYQIPSLARPGCGVVVSPLIALMQDQVSALEPLGIRAAYLNSTLDQAAVREVEKAFR
                     TGKLDLLYVAPERLVQERTLALLDEAEVALFAIDEAHCVSQWGHDFRADYLKLDVLGE
                     RYPGVPRIALTATADERTREEIRARLGMTAATQFVAGFDRPNIRYGIALKDNPRSQLL
                     DLLNRHGGEAGIVYCLSRKKTEDTAAWLVERGFKALPYHAGLDASVRAEHQARFLRED
                     GLIMVATIAFGMGIDKPDVRFVAHLDMPKTVEAYYQETGRAGRDGEPAEAWLVYGLQD
                     VIKLRQMMATSEGSEAHKLAERQRLNAMLGLCEITTCRRQALL"
     gene            complement(1186..>2217)
                     /locus_tag="AC16PP_01935"
     CDS             complement(1186..>2217)
                     /locus_tag="AC16PP_01935"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6480412.1"
                     /translation="RNEPLVENTQVKGTSDTHPLLSPNDEWADFELMELRVASTLPSQ
                     PHGSYVREAYLNGLRMEEESGFNPYRFGVIGSSDTHNAAGSFEEDNYWSKTGITDIEP
                     QLRGSVPLPDSPPDEPKYADATAQYWGASGLAGVWAEANTREAIFEAFRRKETFSTSG
                     PHIRVRFFAGYDYDADILAAGDVLEQAYAGGVPMGGDLLADGDRAPSFLIWAARDPDS
                     APLERLQIVKGWIENGEPQETVVDVACSDGSAPSPMNHRCADNGATVDLSSCAINEDV
                     GAGQLTVRWTDPDFDPGQRAFYYVRVLENPKCRWSTWDAIRAGTPPNPRLHATIQDRA
                     WTSPIWFRP"
BASE COUNT          367 a          758 c          719 g          373 t
ORIGIN      
        1 gcagcagcgc ctggcgccgg caggtggtga tctcgcagag cccgagcatg gcattgagcc
       61 tctgccgctc ggcgagcttg tgcgcctcac tgccttcgga cgtggccatc atctggcgga
      121 gcttgatcac gtcctgcagg ccgtagacca gccacgcttc cgcaggctcg ccgtcgcgac
      181 ccgcacggcc cgtctcctgg tagtacgcct ccaccgtctt cggcatgtcc agatgggcaa
      241 cgaagcgcac gtcgggcttg tcgatcccca tgccgaaggc aatggtcgcc accatgatca
      301 gcccgtcctc gcgcaggaat cgggcctggt gctcggctcg aaccgaggca tccagaccgg
      361 catggtaggg cagcgccttg aaaccccgct cgaccagcca ggcggcggtg tcctccgtct
      421 tcttccggga caggcagtag acgatgcccg cctcaccccc gtgccgattc aacaggtcga
      481 gcagctgcga acgcggattg tccttcagcg cgattccgta gcggatgttg ggccgatcga
      541 agccggcgac gaactgggta gccgccgtca tcccgagccg ggcgcggatc tcctcccggg
      601 tccgctcgtc cgcggtggcg gtcagggcga tccgcggcac accgggatat cgctcgccga
      661 gcacgtcgag cttcaggtaa tcggcgcgga aatcgtgacc ccactgggag acacaatggg
      721 cttcgtcgat ggcaaaaagc gcaacttccg cctcgtcgag cagcgccagg gtgcgctcct
      781 ggacgagacg ctccggggca acgtaaagca gatccagttt gccggtgcga aacgccttct
      841 ccacctcgcg cacggcagcc tggtccagcg tcgaattcag ataggcggcc cggatcccca
      901 ggggttcgag cgcgctgacc tgatcctgca tgagggcgat gagcggcgac accaccacgc
      961 cgcaacccgg gcgggccagg ctcgggatct gatagcacag cgacttcccg ccccctgtag
     1021 gcatcagcac cagggcgtcg cggcccgcca tcaccgtgtc gatgatggcg tcctggggcg
     1081 gacggaacgc tgggtacccg aaggtctgct gaaggatctg cctgggcgtc atggcccagg
     1141 caatataccc gaagagcaga gcgtcctcgg gcgttgcacc ggacgctagg gcctaaacca
     1201 gatcggcgag gtccaggccc gatcctgaat cgtggcgtgg agcctggggt tgggcggcgt
     1261 gccggcccgg atcgcgtccc aggtggacca gcggcacttc gggttctcca gcacccgcac
     1321 gtagtagaag gcccgctggc caggatcgaa atccggatcc gtccaccgca cagtcagctg
     1381 cccggcgccc acgtcctcat tgatcgcaca ggaggacaga tcgactgtcg cgccgttgtc
     1441 cgcgcagcga tggttcatcg gtgacggagc cgagccgtcc gaacatgcca cgtcgacgac
     1501 cgtctcctgt ggctcgccgt tctctatcca gcctttgacg atctgcagcc gctcgagcgg
     1561 cgcgctgtcc ggatccctgg ctgcccagat caggaagctc ggcgcccggt cgccgtctgc
     1621 cagcagatct ccacccatgg gcacgccgcc ggcgtaagcc tgctcgagca cgtcgccggc
     1681 cgcgaggatg tcggcatcgt agtcgtaacc ggcaaagaac cgcacgcgga tgtgcgggcc
     1741 actggtggag aacgtctcct tgcgccggaa ggcctcgaag atggcctcgc gcgtgttcgc
     1801 ctccgcccac acgccggcga gcccggaagc cccccagtac tgggccgtcg catctgcata
     1861 cttcggctcg tccggcggcg agtccggcag cggcaccgac ccgcgcagct gcggttcgat
     1921 gtcggtgatg ccggtcttgc tccagtagtt gtcttcttcg aaggaacccg ccgcgttgtg
     1981 cgtatccgag gagccgatca cgccgaaccg gtacgggttg aagccgctct cctcctccat
     2041 cctgagcccg ttcagatagg cttcgcgcac gtagctgccg tgcggctgcg agggcagcgt
     2101 ggaggcaacc cgcagctcca tcagctcgaa gtcggcccac tcgtcgttcg gcgaaaggag
     2161 cggatgagtg tccgaggtac ctttgacctg agtgttctcc accagtggtt cgttgcg
//
LOCUS       DBNHKV010000069         7704 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1073502, whole genome shotgun sequence.
ACCESSION   DBNHKV010000069 DBNHKV010000000
VERSION     DBNHKV010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1073502"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /gene="purH"
                     /locus_tag="AC16PP_01940"
     CDS             <1..1038
                     /gene="purH"
                     /locus_tag="AC16PP_01940"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007945217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR6480413.1"
                     /translation="RALAAKAFRHTARYDALVAGYLTAEQEYPESLTLSYRHVETMRY
                     GENPHQQAAFYRESGARPGTVAALRQLQGKSLSYNNVADTDAALECVKVFERPACVIV
                     KHANPCGVAEAADVQAAYERAFATDPTSAFGGIIAFNRPLDEPTLTLILERQFVEVIV
                     APEAAPEAVAAAAARKNVRLLVTGPLQAGSRGLELKRVGGGLLVQTPDETCLGEEGLR
                     VVSRRAPSASELDDLLFAWKVAWFVKSNAIVYARDRQTIGVGAGQMSRVVSARIAGFK
                     AAEEGLEVAGSVMASDAFFPFRDGIDAAAEAGIAAVVQPGGSVRDDEVIAAADEHGMA
                     MVFTGIRHFRH"
     gene            1075..2355
                     /gene="purD"
                     /locus_tag="AC16PP_01945"
     CDS             1075..2355
                     /gene="purD"
                     /locus_tag="AC16PP_01945"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009041976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR6480414.1"
                     /translation="MNVLVIGSGGREHALAWKAAQSDGVETVFVAPGNAGTATEAGCE
                     NVDIAVDDFPALAEFAAANGVGLTIVGPEVPLVEGVTDYFEGRGLACFGPSKMAAQLE
                     GSKAFTKAFLDRHRIPTARYQAFTDLAAAQAYVREQGVPIVIKADGLAAGKGVVVAET
                     IEQALQALDDMLSGSAFGEAGSRVVIEEFLAGEEASFICISDGTNVVPFATSQDHKAR
                     DDGDKGPNTGGMGAYSPAPVVTAGVYDRIMKEVVNPTVRGMAEDGVPYVGFLYAGLMI
                     MPDGAPRVIEFNCRFGDPEAQPVMMRLRSDLVGLCRQALDGELAGVELDWDPRVALGV
                     VMAAGGYPEAYDKGDAITGIDTADADPRVKTFHAGTRLEDGVLVTNGGRVLCVVGLGD
                     DVAGARAAAYGAVEHIDWRNRYYRRDIGHRALDR"
     gene            2427..3395
                     /locus_tag="AC16PP_01950"
     CDS             2427..3395
                     /locus_tag="AC16PP_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR6480415.1"
                     /translation="MIVRSLRRFEPFARLPSAALIPVAEHASTLSLPAGRWLVRPGRS
                     LPGHYYLQRGAVRLREPDQRVAAGSPRSRWPIYPGAQGVLTLSPVTLVRVSEDAAAAT
                     AHAREVQPPLSRPQWLTDGWECRFLSSGAMGRVSLDHCQRVMRAMRPVDLLRGEMVVR
                     TGEPGDTVFVLAAGRAEVSRGRRSLALLEPGDHFGEDAAISGACRNACVTMVSDGRVM
                     TLSGELFRDFVVGRVLLDDGDGPAQATIDIGGRSVSAGLGIPLLLLRERLGLLTPELR
                     YRLAGGSLRQRVLAAFILLHRGFRVALAESGLDAEVALHPEPRAHL"
     gene            3470..3814
                     /locus_tag="AC16PP_01955"
     CDS             3470..3814
                     /locus_tag="AC16PP_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR6480416.1"
                     /translation="MSSDCLFCRIASGEIPGDIVYEDDELLAFNDISPQAPHHVLVIP
                     KIHVATVNDLAAEHADLVGRLVLCARAIAAERNIQESGYRLILNCNGHGGQTVFHLHL
                     HLLGGRQLMALG"
     gene            3816..4454
                     /gene="coq7"
                     /locus_tag="AC16PP_01960"
     CDS             3816..4454
                     /gene="coq7"
                     /locus_tag="AC16PP_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HIR6480417.1"
                     /translation="MAGPGSLADALVAQVDKALRTLTGAAASVRENPAADVPEGEMSP
                     GEREHAAGLMRVNHTGEICAQALYEGQALTASDPRTRTALLAAASEESDHLAWCAGRL
                     AELGSSPSVLNPAFYALSYALGAVTGVLGDKVSLGFVEATEDQVCRHLESHLQSLPAG
                     DSRSRQIVRAMREDEARHGAEALGAGGAEFPQPVKRAMSLASRLMTETTYRV"
     gene            complement(4442..4855)
                     /locus_tag="AC16PP_01965"
     CDS             complement(4442..4855)
                     /locus_tag="AC16PP_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012274236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6480418.1"
                     /translation="MTKPPKPHAGLRHVALFTTTLEASVRFYTELLGMQVEWNPDPDN
                     FYLTSGTDNLALHRATEGRSVRGQTLDHIGFIIDDEEDVDAWHDHLAANGVNILERPR
                     THRDGARSFYCEDPDGNVVQLIYHPPISRPGGQTR"
     gene            complement(4875..5294)
                     /locus_tag="AC16PP_01970"
     CDS             complement(4875..5294)
                     /locus_tag="AC16PP_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014872825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR6480419.1"
                     /translation="MQAKITLIENVHFEAVADSGHTVLIDGPPDAGGENRGTRPMELV
                     LMGLGGCASFDVVSILRKSRQPVVGCVTRLTARRADTVPQVFEAIHLHFTISGQGLDE
                     AKVARAVALSAEKYCSASIMLERGGVAISHDYEILEA"
     gene            5494..6153
                     /gene="crp"
                     /locus_tag="AC16PP_01975"
     CDS             5494..6153
                     /gene="crp"
                     /locus_tag="AC16PP_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HIR6480420.1"
                     /translation="MGKPLTAKGFNPNAGQEDTHLDAFLAAAHRRKYPAKSTLIYAGD
                     KSDSIYYILKGSVTVLVEDESGREIIVAYLNKGDFFGEMPLFTESAPRSAWIKAKTEC
                     EVAELSYGKFRELAQRHSDLLYAVGRQMADRLGRTTQKVSDLAFLDVTGRVARTLIEL
                     TKQPDAMTHPDGMQIKITRQEIGRIVGCSREMVGRVLKTLVEQGLVNVKGKTMVVYGT
                     R"
     gene            complement(6298..7083)
                     /gene="trpC"
                     /locus_tag="AC16PP_01980"
     CDS             complement(6298..7083)
                     /gene="trpC"
                     /locus_tag="AC16PP_01980"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR6480421.1"
                     /translation="MTILDEILAHKRTEVAERQRVRSLDSLRALAQACPPARGFADAL
                     ESKLLAGASGVIAEIKRASPSKGVIREDFDPVSIARSYAKGGAACLSVLTDERYFQGH
                     DEYLQAARAAVDLPVLRKDFVVDAYQLFEARAMGADCVLLIAAALDAGQLKALHETAI
                     ALGLDVLVEVHDGEELESALEIEPRLVGINNRNLKTFETRLETTYGLLPGIPDSVTVV
                     TESGIANREDVAAMRQHGVHCFLVGEAFMRAPDPGTALAEMFA"
     gene            complement(7131..>7704)
                     /gene="trpD"
                     /locus_tag="AC16PP_01985"
     CDS             complement(7131..>7704)
                     /gene="trpD"
                     /locus_tag="AC16PP_01985"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:NF012800.8"
                     /note="Catalyzes the conversion of
                     N-(5-phospho-D-ribosyl)-anthranilate and diphosphate to
                     anthranilate and 5-phospho-alpha-D-ribose 1-diphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR6480422.1"
                     /translation="LFAQAHHSAMRHAAPVRQELGARTIMNVLGPLTNPAGARNQVLG
                     VFSAAWQTTLAEVLKLLGASHVLVVHSNGLDELALNGPSFVVELNAGTITEYEIEPAD
                     FGLKPSSHDELRADSPETSLALLKRSLTEPDSSAADIVSLNAGAAIYAAGVATSFKNG
                     VVMAQDAIAAGLANERLAELVRVSSMMAEP"
BASE COUNT         1245 a         2470 c         2638 g         1351 t
ORIGIN      
        1 cgcgcgctgg ccgcgaaggc cttccgccat acggcccgct acgatgcgct cgtggccggt
       61 tacctcaccg cggagcagga gtacccggag tcgctcacgc tcagctatcg ccacgtggag
      121 accatgcggt acggggagaa tcctcatcag caggcggcct tttaccgcga gtcgggggcg
      181 cgaccgggca ccgtggccgc cctgcgccag ctgcaaggca agagcctgtc ctacaacaac
      241 gtggccgata ccgatgccgc gctggagtgc gtcaaggtgt tcgagcggcc ggcctgcgtc
      301 atcgtcaagc atgcaaaccc ctgcggcgtt gcggaggccg ccgacgtaca ggccgcatac
      361 gaacgtgcct tcgcgacgga tccgacctcg gcgttcggtg gcatcatcgc cttcaacaga
      421 ccgctcgacg agcccacgtt gacgctgatt ctcgagcggc agttcgtgga ggtcatcgtc
      481 gcgcccgagg cggcgccgga ggcggtggcc gctgccgcgg cccggaaaaa cgttcgtctt
      541 cttgtcaccg gccctttgca ggcaggctcc cgcgggctcg agctgaagcg cgtgggtggc
      601 gggttgctcg tccagacgcc cgacgagacc tgcctgggtg aagaggggct gcgggtggtg
      661 tcgcggcgcg cgccgtctgc cagcgagctc gacgacctgc tgttcgcctg gaaggtggct
      721 tggttcgtca agtccaacgc catcgtctat gcccgcgacc gccagaccat cggcgttggc
      781 gcgggtcaga tgagcagggt ggtgagcgcc cgaatcgcgg gattcaaggc agccgaggag
      841 gggctggagg tagccggctc cgtgatggct tccgatgcct tctttccctt ccgggacggc
      901 atcgacgcgg ctgccgaggc cgggatcgcg gcggtggttc agccgggcgg gtccgttcgc
      961 gacgacgagg tcatcgcggc ggccgacgag cacggcatgg ccatggtgtt caccggcata
     1021 cgtcacttcc ggcattagcc tttgcggggc catcgaacct tagaggatgc ctagatgaat
     1081 gtgcttgtca ttggcagcgg cggcagggag cacgccctgg cctggaaggc cgctcagagc
     1141 gacggcgtgg agacggtctt cgtggcaccg ggaaacgcgg gaacggcgac ggaggccggg
     1201 tgcgagaacg tcgacatcgc ggtggacgat ttccccgccc tggcagagtt tgccgccgcg
     1261 aacggcgtag ggctcaccat cgtcgggccg gaagtccccc tcgtggaagg cgttaccgat
     1321 tacttcgagg gccgcgggct cgcctgcttc gggccgagca agatggcggc ccagctggaa
     1381 ggctccaagg ctttcaccaa ggcgtttctg gaccgtcacc ggatcccgac cgcgcgctac
     1441 caagccttca ccgatctggc ggccgctcag gcctacgttc gcgagcaggg cgtgcccatc
     1501 gtcatcaagg cggatggcct ggcggccgga aagggggtgg tggtcgcgga aaccatcgag
     1561 caggcccttc aggcgctgga cgacatgctc agcggatcgg cttttggcga agccggatcc
     1621 agggtggtga tcgaggagtt cctggctggc gaggaggcga gcttcatctg catatcggac
     1681 ggcaccaacg tggtgccgtt cgccacgtct caggatcaca aagcccggga cgatggggat
     1741 aaggggccca acacgggcgg catgggcgcc tactcgcctg cccccgtggt aactgccggc
     1801 gtctatgacc gcatcatgaa ggaggtggtc aaccccacgg tgcgtggcat ggcggaggac
     1861 ggcgttccct acgtcggctt cctctacgcc gggctcatga tcatgcccga cggtgcgccc
     1921 agggtcatcg agttcaactg ccggttcggc gatccggaag cccagcccgt gatgatgcgc
     1981 ctgcgctccg atctggtcgg tctctgccgg caggcgctgg atggggaact cgccggcgtc
     2041 gagctcgact gggacccgcg ggtggcgctc ggcgtcgtga tggctgcggg ggggtacccc
     2101 gaggcgtacg acaagggtga cgccattacc ggcatcgata ccgcagacgc agatccgcgc
     2161 gtgaagacct ttcatgccgg cacccgtctc gaagacggcg tgctggtgac caacggtggc
     2221 cgcgttctct gcgtcgtggg gcttggggac gacgttgccg gcgcccgtgc ggccgcgtac
     2281 ggagccgtcg agcacatcga ctggcggaac cgctactacc ggcgcgacat cgggcatcga
     2341 gcgctcgaca gataacggca atttccgtca cgccaacaat ctgaaggcgg cggcgccgca
     2401 gctccgcgcc gcgggaaaat ggcgccatga ttgtccggtc gctgcgccgc ttcgaaccat
     2461 ttgctcgact cccgagcgcg gcgctcattc cggtggccga gcacgccagc accttgagct
     2521 tgcccgcggg ccgttggctg gtcaggccag gccggtcgct gcccgggcac tactatctgc
     2581 agcgaggagc ggttcgtttg cgggaacctg accaacgggt tgctgccggg tcgccgcgat
     2641 cccggtggcc catctacccg ggcgctcagg gcgtgctcac cctatctccg gtcaccctcg
     2701 tgcgcgtgag cgaagatgcg gcggccgcca cggcccacgc ccgggaagtc cagccgccgc
     2761 tctcccggcc ccagtggttg accgacgggt gggagtgccg ctttctcagc tccggagcca
     2821 tggggcgggt cagcctggac cactgtcaga gggtgatgcg ggccatgcgc cccgtggacc
     2881 tcctccgggg cgagatggtg gtgcgaaccg gggagcccgg cgataccgtc ttcgtgcttg
     2941 cggccggccg agccgaggtc agcagagggc ggcgttcgct tgcgctgctc gagccgggtg
     3001 accatttcgg ggaggatgcg gccatcagcg gtgcttgccg caacgcttgc gtgaccatgg
     3061 tctccgacgg tcgcgtgatg acgctctccg gtgagctgtt tcgcgacttc gtggtcgggc
     3121 gggtgcttct cgacgacggc gatggccccg cccaggcgac catcgacata ggcggacggt
     3181 ccgtgtcggc gggcctgggt atacccctcc tgctgctcag ggagcggctc gggttgctga
     3241 cgcccgagct gcgctaccgt ctggcgggtg gcagtctccg gcagcgcgtg ctggctgcct
     3301 tcattctcct tcatcgcggc tttcgcgtgg cgcttgccga gagcgggctg gacgccgaag
     3361 tcgcgctcca tcctgagccg cgcgctcatc tttaagcccg cgttgcgggg gctgttgtgc
     3421 cggtttacag tcacctctcc cggcatgttg accgaagcgc gagaagcgaa tgagttccga
     3481 ttgcctgttc tgcagaatcg cgtccggcga gatacccggc gacatcgtct acgaggacga
     3541 tgagctgctc gccttcaacg acatcagccc gcaggcgccg catcacgtgc tcgtcatccc
     3601 gaagattcac gtcgccaccg tgaacgacct tgcggccgaa cacgccgatc ttgtcggtcg
     3661 tctggtgctc tgtgcccggg ccatcgccgc cgagcgcaac attcaggagt cgggctaccg
     3721 cctgatcctc aactgcaacg ggcacggcgg ccagacggtc ttccaccttc acctccacct
     3781 gctgggcggc cggcagctga tggcgctggg ctgacgtggc tggtcctggc tctttggctg
     3841 atgccctggt ggcgcaggta gacaaagcgc tgcgcacgct gacgggcgct gcagcttcgg
     3901 tccgtgagaa tcccgcggcc gatgtgcctg aaggagagat gagccctggc gagcgggagc
     3961 acgctgccgg cctgatgagg gtgaatcaca ccggcgagat atgcgcccag gcgctctacg
     4021 agggccaggc gctgacggcg tcggaccccc gcacccggac agcgctgctc gcggcagcct
     4081 ccgaagagtc ggaccacctc gcctggtgtg ccggccggct cgccgagctg ggcagcagcc
     4141 cgagcgtgct caacccggcc ttctacgcgc tttcctatgc cctgggggcg gtgacgggcg
     4201 ttctcggtga caaggtcagt ctgggattcg tggaggcgac ggaggatcag gtctgccgac
     4261 acctcgagtc gcacctgcag tccctgccgg ccggcgattc ccgcagccgt cagatcgttc
     4321 gcgccatgcg ggaagacgaa gcgcgccacg gggccgaggc cctgggagcc ggcggggccg
     4381 agtttcccca gccggtgaaa cgggcgatgt cgctggcgtc caggctgatg accgagacta
     4441 cctaccgcgt ctgaccaccc ggtcgggaaa tcggcggatg atagatgagc tgcaccacgt
     4501 tgccgtcggg gtcctcgcag tagaacgatc gcgcaccgtc gcgatgggtc ctgggtctct
     4561 cgaggatgtt cacgccgttg gcggccaggt ggtcgtgcca ggcgtccaca tcttcctcgt
     4621 cgtcgatgat gaagccgatg tggtccaggg tctggccgcg cacggagcgg ccctcggttg
     4681 cccggtgcag cgccaggttg tccgttccgg acgtgaggta gaagttgtcg ggatccggat
     4741 tccactccac ctgcatgccg aggagctcgg tgtagaagcg cacggaggct tccagcgtgg
     4801 tggtgaacag cgccacgtgc cgcagccctg catgcggctt gggtggtttg gtcatctgcg
     4861 gaggttccgg agcttcatgc ttccagtatt tcgtagtcgt ggctgattgc aacaccgccg
     4921 cgctcgagca tgatcgaagc ggagcagtac ttctccgccg acagcgccac ggctcttgcg
     4981 accttggcct cgtccaggcc ctgcccgctg atggtgaagt gcagatgaat ggcctcgaac
     5041 acctggggta ccgtgtcggc gcgccgggct gtgagcctgg tcacacagcc gacgacgggc
     5101 tgccgcgact tcctgagaat gctgaccacg tcgaacgacg cgcagccgcc cagacccatg
     5161 agcacgagct ccatcggccg ggtacctctg ttctcgccgc ccgcgtccgg cggaccgtcg
     5221 atgagcaccg tgtgcccgga gtctgccacg gcttcgaaat gaacgttctc gatcagcgtg
     5281 atttttgctt gcatggccgg cagtttccga tatctggatg cccgatacgt gaggctggga
     5341 gcgtctgagt agtgatgggg gaccactcat actgtgaaat ggatacgttt gtcgaccagt
     5401 tctcgttgtg gttgtaaacg atatgcaagt attcatccgc aaggttcaca aggagcggaa
     5461 atgcttgcca acaagaattc acagttctca gggatgggaa aaccactgac cgccaagggg
     5521 ttcaacccga acgccgggca ggaagatacg catctggatg cctttctggc agcggcccat
     5581 cgccgcaagt atccggccaa gagcacgctc atctacgccg gtgacaagag cgattcgatt
     5641 tactacatcc tcaagggctc ggtaaccgtg ctcgtggagg acgaatccgg acgtgaaatc
     5701 atcgtcgcct atctcaacaa gggcgatttc ttcggcgaga tgccgctttt cacggaaagc
     5761 gcgccgcgtt cggcctggat caaagccaag accgagtgcg aagttgccga actcagctac
     5821 ggcaagttca gggaactcgc ccagcgccac agcgatctgc tctacgccgt gggccggcag
     5881 atggccgatc ggctcggccg cactacccag aaggtcagcg atctggcgtt cctggacgtg
     5941 accggccgcg tcgcgcgtac gctgatcgag ctcaccaagc agcccgatgc catgacccat
     6001 cccgatggca tgcagatcaa gatcacccgc caggagatcg gccgaatcgt cggatgctcc
     6061 cgcgagatgg tggggcgtgt tctgaagacg ctggtcgagc agggtctggt gaacgtcaaa
     6121 ggcaagacga tggtggttta cggcacccgc taagacccca gcaggcgcgc cgaaaggaaa
     6181 cggcgcgccc ggttgctcac acaggacgtg gaacagcggg ctctctgcca gatccggcgg
     6241 agagcccgtt ttccgtttct gctgcaggga cgcggccgag ggtcgagccc ggcggtccta
     6301 agcgaacatc tcggcgagcg ccgtccccgg atccggcgcg cgcatgaacg cttcgccgac
     6361 gaggaagcag tgcacgccgt gctgccgcat ggccgcgaca tcctcgcggt ttgcgatgcc
     6421 gctctccgtc accaccgtca cgctgtcggg aatgcccggc aggagtccgt aggtggtttc
     6481 aagccgcgtc tcgaaggtct tcaggttgcg gttgttgatg ccgaccaggc gaggctcgat
     6541 ctccagggcc gactcgagct cctccccgtc gtggacctcc acgagcacgt ccagaccgag
     6601 cgcgatggcc gtttcgtgca gggccttgag ctgtcccgcg tcgagggcgg ccgctatgag
     6661 aagcacgcag tccgcgccca ttgcgcgtgc ttcgaacagc tgatatgcgt ccaccacgaa
     6721 atccttgcgc agcacgggca ggtccacggc cgctcgggcg gcctgcaggt actcgtcgtg
     6781 gccctggaag tagcgctcgt cggtaagaac ggagaggcag gcggcgcccc ccttggcgta
     6841 gctccgggca atggacacgg ggtcgaaatc ttcccgtatg acgcctttgc tgggcgatgc
     6901 tctcttgatc tctgcgatca cgcctgaagc gccggccaaa agcttgcttt ccagcgcatc
     6961 tgcaaagccg cgggcgggcg ggcacgcctg ggccagggcg cgcagggaat ccaggcttcg
     7021 cacccgctgg cgctcggcca cctcggtgcg tttgtgggcg agaatctcgt cgagaatggt
     7081 cacggcgccg ccaccgtcac ggctcttcta ccgtcacggc tcttccacct tcacggctcc
     7141 gccatcatgg aagagacccg aacgagctcg gcaaggcgtt cgttggcgag ccctgcggcg
     7201 atggcgtcct gggccatgac cacgccgttc ttgaagctcg tggcgacgcc ggccgcgtag
     7261 atcgccgccc ccgcattgag gctgacgatg tcggcggccg atgagtcggg ctcggtcagg
     7321 gagcgtttca gcagggcaag gctggtctcc ggagaatccg ctcgcagctc gtcgtgggag
     7381 gaaggcttca ggccgaagtc ggcgggctca atctcgtact cggtgatggt gccggcgttg
     7441 agctccacca cgaagctcgg cccgttcagg gcgagctcgt ccaggccgtt gctgtgcacc
     7501 accagcacat ggctcgcgcc gagcagcttg aggacctcgg ccagggtggt ctgccaggcc
     7561 gcgctgaaga cgcccagcac ctggttgcgc gcgccggccg ggttggtcag cggccccagg
     7621 acgttcatga tcgtccgtgc tccaagctcc tggcgaacgg gcgcggcgtg acgcatggcg
     7681 ctgtgatgag cctgcgcgaa cagg
//
LOCUS       DBNHKV010000070         7015 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1073846, whole genome shotgun sequence.
ACCESSION   DBNHKV010000070 DBNHKV010000000
VERSION     DBNHKV010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1073846"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="AC16PP_01990"
     CDS             <1..772
                     /locus_tag="AC16PP_01990"
                     /inference="COORDINATES: protein motif:HMM:NF025598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HIR6480423.1"
                     /translation="SLGSGAPADAEKIGEVLEKLTDIEPGWPVAATEASRERFRVTGD
                     DHERRVALFAGEGKLAEIYLGTSPGFRRIHARRADSEEVYSIDFAVYELPLSDSDWLD
                     KDLLGADGEIASIIREEHWSLERGQEGWLLNLVPGMQDAEAGPGIGGADQDAAGRLVE
                     RVAELRVTGFAPLGPAYEPAAVFTVTDASGSYGLRIFKDPENDEYAVESDRLSGRFGL
                     AAYVAEQLVVEHADLRAAEPQDEAPAPGSEGFSDAGPG"
     gene            884..1651
                     /locus_tag="AC16PP_01995"
     CDS             884..1651
                     /locus_tag="AC16PP_01995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6480424.1"
                     /translation="MIARRNSQRSNSAELLEFGEYVKVLEASGEREDVVAFFDLDRTI
                     IAGYSITALMIERLRSGMSPRHLVAQVSRFISYGFGRAGYQELLEGTVAELVGLPEQE
                     LVELGERAYQRRLQRLIYPEARRLIGAHKELGHEVVMVTSATRYQAQPVARELAVDQI
                     CCTELEIRQGRITGRVSPCFGASKRAAAVHLGSARACSLADAYFYTDSCDDLPLLEAV
                     GRPVAANPKASLSEVATSRGWPQLAFRRQGEAGSVAA"
     gene            1653..2282
                     /locus_tag="AC16PP_02000"
     CDS             1653..2282
                     /locus_tag="AC16PP_02000"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009394438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6480425.1"
                     /translation="MIDLYTWPTPNGRKISIALEEFGEPYRVHAVDIGAGDQHGSAFR
                     RISPNGKIPAIVEDSSGRSLMESGAILLYLAEKHDRFRGDDWWQTVEWLMLQMASVGP
                     MLGQVHHFARFNPGKAPYAEARYLDEARRLYGVLDRRLADREFLSGSYSIADMASWPW
                     ISRYEWQGIDLADYAHVRRWYVSIAGREAVQRGYHVPHFVNPVPMPDGA"
     gene            2377..3606
                     /locus_tag="AC16PP_02005"
     CDS             2377..3606
                     /locus_tag="AC16PP_02005"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012076215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR6480426.1"
                     /translation="MREAWIIDAVRSPRGRGKPGVGSLSNIHPQRCMAQVLNGLRDKV
                     GFDASDIEDVVAGCGTGSGDHAHDIARMSVLDAGWPLTTTGVTLHRFCGSGQQAVTFA
                     AMGVMSGHQEAVIGGGVEFMSRYAPIHAMGFHANNEHLAALHPQVPQGISADLIATIE
                     GFSREDVDGYAVESQTRAARSIAEGRFEKALIPIFNDDGSPALDRDEHPRPGTTLESL
                     ADLKPSFAAMGSQQVEGFPKPFDEMARDRYPQVEEINHVHHAGNSSGVVDGASAVLVV
                     SPDFAKAHGLRPRARIRMTATAGTEPLIMLTAPAPAAQRCLQKAGMTRDDIDLWEINE
                     AFASVVLKFMKEMGVDHDVVNIDGGAIALGHPIGATGGMLIGTAVDQLEREDKETALV
                     AMCTGGGMGTATILERI"
     gene            complement(3640..6204)
                     /locus_tag="AC16PP_02010"
     CDS             complement(3640..6204)
                     /locus_tag="AC16PP_02010"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6480427.1"
                     /translation="MRAFSSFTAPLLVLLALSAFAQPTTDYLTPDLRARVERLKADVQ
                     QVPTNTTNVAERSRVLWNWANALSLDGGVLPVNLTQAVSFVFAYPDLMADRHDVIDGF
                     IQELTLLDEEPGALGTLRADSGPFQAASFVTLRQTFTVGSKAIQAGGGILVARHFQPN
                     YGAWQTGDPAADNYLSITTSAADASFTTETAPMIGMHGGFRRPADALVFRVASGTLRP
                     GDTVTLTYGDRSQGSRGFLMADFSSDMMPLPLYVAFTGSGRFFSLPIQPVAVIGGPAA
                     GVHAFAPSIVKAGEPFTLAVRAEDRFYNRASDGHPDWLVSLNGEPFREVPASEEPITL
                     LRNIEIAEPGVYRFTIASRDGQISGRGNPILVKQTAEERIFWGDTHGHSGFAEGIGTP
                     DFFMRWARDDARLDFVTHSEHDIWLDDGEWEQLRNNARSYTREGEFVAFLGYEWTVRN
                     THGGHHNVLFRTPAGRNRLAAPFYPSLSKLYAGLRGDAQPEDVLVIPHAHQAGDYRTS
                     DPELEHLVEVNSQHGNFEWFGQAYLRHGHQVGFTAASDNHLSQPGYGAPKNGTLAQRG
                     GLGAVIARARTTDALFDAMKQARAYATSGERIILEFSVNGTPMGQRGPFSETRTIEGR
                     VIGTAPIDDISLLRNGEEIWRRELLTDAADNTSSPGAFLLTFASDSDPRQPGDNPRGW
                     RHWRGTIEVRDATLIDASGMDFNNQLLQQIEADAGDPDLLRFSTITRGDTSSIRLDLE
                     DVSRATQVRVTLEPTQETGGAPATYGRHEQLPAAEVKLEIRDFENGETRAQLPAGPRE
                     DTITLRRIITQGSMDEAFTIDDVSGRQGDYYYVRVRQADDSLAWSSPVWIGGHAPR"
     gene            complement(6287..>7015)
                     /locus_tag="AC16PP_02015"
     CDS             complement(6287..>7015)
                     /locus_tag="AC16PP_02015"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6480428.1"
                     /translation="HQVLFFPDQPMSHEDLERFTLCFGAFGNDPYVKAIPGHPHILEV
                     RREPGENVAPFGASWHSDWSFQAQPPAATILHAKIVPPVGGETWYADGYAAYEALPAT
                     LRDEISELTAIHSARRPYSHQGFLAGRGPERSMTILPSDEALQTQDHPLVRTHPETGR
                     KALWVNGVYTIGIRNMAEEDAKNLLKRLFDHAIRDEFVYRHRWAPDMLTMWDNRSVQH
                     CARGGYDGYRRVMHRTTVAGDSPV"
BASE COUNT         1154 a         2199 c         2437 g         1225 t
ORIGIN      
        1 gtcgttgggc tccggcgccc ctgccgacgc ggagaagatc ggcgaggttc tggaaaaact
       61 caccgacatc gagcccggct ggcccgtggc ggcaaccgaa gcgtcgcggg agcggtttcg
      121 cgttaccggc gatgatcacg agcgacgggt ggccctcttc gcgggggaag gaaagctggc
      181 cgagatctat ctgggaacgt cgccgggatt ccgccgcatt catgcccgac gggcggattc
      241 cgaggaggta tacagcatcg atttcgccgt ctacgagctg ccgttgagcg attcggattg
      301 gctcgacaag gacctgctgg gggcagatgg ggagatcgcc tcgatcatcc gggaggaaca
      361 ctggtccctg gagcgtggtc aggaaggctg gctcctgaac ctggtgccgg ggatgcagga
      421 tgccgaagcc ggccccggaa tcggcggcgc cgatcaggac gccgcgggcc ggctcgtcga
      481 gcgggtcgcc gagcttcggg tgaccggatt tgcgccgttg ggccccgcgt acgagcctgc
      541 tgcggtcttc accgtcacgg acgcatccgg gtcttacggg ctgcggattt tcaaggatcc
      601 ggagaacgac gagtacgccg tggaatccga tcgtctgtcc ggccgcttcg gtctggccgc
      661 ttacgtagcc gagcagctgg ttgtagagca tgccgacctg cgtgccgccg aaccgcagga
      721 cgaagcgccg gcgcccgggt ccgaagggtt ttccgatgcg ggccccggtt gaatcgactc
      781 gtgtttcgat ggtctcattg ttcaggcgtc gccgacgtgc ggagcgcacc gcgctggcgc
      841 ggcgcgtgag agctaaaccg accaacggcg accagggcct gccatgattg cccggcgaaa
      901 cagtcaaagg tcgaactccg cggagctgct ggagttcggc gagtacgtca aagttctgga
      961 ggcgtctggc gagcgcgagg acgtggtcgc gtttttcgat ctggatcgaa ccatcatcgc
     1021 cggctattcc atcaccgcgc tcatgatcga gcggcttcga agcggcatgt cgccacggca
     1081 tctcgtcgct caggtcagtc gcttcatctc ctatggcttt ggccgggccg gttaccagga
     1141 actgctcgaa ggaaccgtgg ccgagctcgt agggctgccg gagcaggagc tggtggagct
     1201 gggggagcgg gcctaccagc gccgtctgca gaggctgatc tacccggagg cgcgccgcct
     1261 gatcggcgcg cacaaggagc tgggtcacga agtcgtcatg gtcacttcgg ctacccggta
     1321 tcaggcgcag cccgtcgccc gggagctggc cgtcgaccag atctgctgca cggagctgga
     1381 aatccgccag ggcaggatca ccggcagggt cagcccgtgt ttcggtgcga gcaaacgggc
     1441 ggcagccgtt cacttggggt ccgctcgggc ctgcagcctg gcggacgcct atttctacac
     1501 cgattcctgt gacgacctgc cgcttctcga agccgtcgga cggcccgtgg ctgccaaccc
     1561 caaggcttcg ctttccgagg tcgcaacgtc gcgcggctgg ccgcagctcg cgttccgcag
     1621 gcagggcgag gcgggctcag tggccgcctg aggtgatcga cctttacacc tggccgacac
     1681 ccaacggccg caagatctcg attgctctgg aagagttcgg cgagccctac cgggtccacg
     1741 ctgtggacat cggcgcgggc gatcagcacg gctcggcctt ccgacgaatc agcccgaacg
     1801 gaaaaatccc ggccatcgtc gaggattcgt ccgggcgcag cctgatggag tccggggcca
     1861 tcctgctcta cctggctgag aagcacgacc gcttccgggg cgacgactgg tggcagacgg
     1921 tggagtggtt gatgctgcag atggcaagtg tcggccccat gctcgggcag gttcatcact
     1981 tcgccagatt caatcccggt aaagccccct acgccgaagc gcgctacctc gacgaagcgc
     2041 ggcgcctata cggcgtgctc gatcggcgcc tggcggatcg ggaattcctg tcgggcagct
     2101 actctatcgc ggacatggcg agctggccgt ggatatcgcg ttacgaatgg cagggaatcg
     2161 accttgcgga ctacgcccac gtgcgccgct ggtacgtctc gatcgccggc cgcgaagccg
     2221 tccagcgcgg ttatcacgtg ccgcacttcg tcaatcccgt ccccatgccg gacggcgcct
     2281 gagcgtcacg ccgccggcgt ggccgcgggc gccggtgaag cctcggttgg cccggtgtag
     2341 aatcgcggct ccactgacat tcaacggaga ggcgccatgc gggaagcctg gatcatcgac
     2401 gcggtgcggt caccgcgggg gcggggtaag ccgggggtcg gcagcctgag caacattcac
     2461 cctcagcgct gcatggcgca ggtgctcaac gggctgaggg acaaggtcgg tttcgatgcg
     2521 tccgacatcg aggacgtcgt cgccggctgc gggaccggca gcggcgatca cgcccacgac
     2581 atcgcccgca tgtcggtgct ggatgccggc tggcccctga cgaccaccgg ggtaaccctg
     2641 caccgattct gtggcagcgg acagcaggcc gttacctttg ccgccatggg cgtgatgtcc
     2701 ggtcaccagg aagccgtgat tggcggcggg gtggagttca tgtcccgtta cgcgcccatc
     2761 cacgcgatgg gctttcatgc caacaacgaa cacctggctg ccctgcatcc ccaggttcct
     2821 caggggatct ccgcggatct gatcgccacc atcgaggggt tcagccggga agacgtggac
     2881 ggctacgcgg tggagagcca gacccgtgcc gctcgctcca ttgccgaggg tcgattcgag
     2941 aaggccctga tccccatctt caacgacgac ggttcgcccg cgctggaccg ggacgagcat
     3001 ccccggcccg ggacgacgct ggagagcctg gctgatctga agccctcgtt tgccgccatg
     3061 ggctctcagc aggtggaagg atttcccaag ccgttcgacg agatggcccg cgaccgctac
     3121 cctcaggtgg aggagatcaa ccacgtgcac cacgccggga actcctcggg cgtggttgac
     3181 ggcgccagcg ccgtgctcgt cgtgagcccg gattttgcaa aggctcacgg tctgcgaccc
     3241 cgagcccgca tccgcatgac ggccaccgcc ggaaccgagc cgctcatcat gttgaccgca
     3301 ccggctcctg cggcccagcg ttgcctgcag aaggcgggca tgacgcggga cgacatcgat
     3361 ctgtgggaaa tcaacgaggc gtttgcttcc gtcgtgctca agttcatgaa ggagatgggg
     3421 gtcgatcacg acgtggtcaa catagacggt ggcgccatcg cgttgggtca tcccatcggc
     3481 gcgacgggcg gcatgctcat cggcacggcg gtggatcagc tcgagcgcga ggacaaggaa
     3541 accgcgctgg ttgcgatgtg cacaggcgga ggaatgggta cggctacgat cctcgagcgg
     3601 atctgaggtc ggctccggcg gggtatccca aggcgccgct cagcgcggcg cgtgcccgcc
     3661 gatccacacc gggctagacc acgccaggct gtcgtcggcc tggcggacgc gcacgtagta
     3721 gtagtcaccc tggcgcccgc tgacgtcgtc gatggtgaaa gcttcgtcca tgcttccctg
     3781 ggtgatgatc cggcgaaggg tgatggtgtc ctcccgtgga ccggcgggca gctgcgcccg
     3841 ggtctcgccg ttctcgaaat ctcttatctc cagcttcacc tccgcggccg gcagctgctc
     3901 atggcggccg tacgtggccg gcgcgccgcc ggtttcctgg gtgggttcca gggtaacgcg
     3961 gacctgcgtg gctctggata cgtcctccag gtcgagccga atgctgctcg tgtccccgcg
     4021 ggtgatggtc gagaaacgaa gcaggtccgg atcccctgcg tccgcttcga tctgctggag
     4081 aagctgattg ttgaaatcca tgccgctggc atcgatgagc gtcgcgtccc tcacctcgat
     4141 ggttccacgc cagtggcgcc accctcgcgg attgtctccc ggctgtcgcg gatcggagtc
     4201 ggaggcgaag gtcagcagga aagctcccgg agagctcgta ttgtcggcag cgtcggtcag
     4261 cagttcccgg cgccagatct cctcgccgtt tcgcagcaac gagatgtcgt cgattggcgc
     4321 cgtgccgatc acccgacctt cgatcgtgcg ggtttcggag aagggtccgc gctgccccat
     4381 gggcgtcccg ttcaccgaga actccaggat gatgcgctcg ccggaagtcg cataggcgcg
     4441 agcctgcttc atggcgtcga acagcgcatc ggtggtcctg gcccgggcaa tgacggcgcc
     4501 cagaccgcct cgctgggcaa gtgtcccgtt ctttggggca ccgtatcccg gctggctgag
     4561 atggttgtct gacgcggcgg tgaagcccac ctggtgcccg tggcgcagat aggcctgacc
     4621 gaaccattcg aagttgccgt gctgggagtt gacctccacg aggtgctcga gttccgggtc
     4681 gctggtgcgg tagtcgccgg cctggtgcgc gtgggggatg acgagcacgt cctctggctg
     4741 ggcatcaccg cgcaggcctg cgtaaagctt tgacagggac gggtagaagg gcgctgccag
     4801 ccgattgcgg ccggcgggcg tgcggaacag cacgttgtgg tgtccgccgt gggtattccg
     4861 gaccgtccac tcgtagccga ggaaggcgac gaactcgcct tcccgggtgt agctgcgggc
     4921 gttgttgcgc agctgctccc actcgccatc gtccagccag atgtcgtgct ccgaatgggt
     4981 gacgaagtcc aggcgcgcat cgtcgcgcgc ccagcgcatg aagaagtcgg gcgtgccgat
     5041 accctccgcg aatcccgagt ggccgtgggt atcgccccag aatatccgct cctcggccgt
     5101 ctgcttgacc aggattggat ttccacgtcc gctgatctgg ccgtcccggc tcgcgatggt
     5161 gaaccgatat acgcctggtt cggctatctc gatgttgcgc aacagggtga tcggctcctc
     5221 gctggccggc acctcccgga acggttcgcc attgagggaa accagccagt ccggatgtcc
     5281 gtccgaggcg cggttgtaga agcggtcctc ggcccggacc gccagggtga agggctctcc
     5341 ggccttcacg atagacggcg cgaaggcgtg cacgccggcc gccgggccgc cgatcaccgc
     5401 cacgggttga atgggtagcg agaagaagcg tccggatccg gtgaacgcca cgtacagggg
     5461 caacggcatc atgtcggagg aaaaatcggc catcaggaat ccgcggctcc cctggctgcg
     5521 gtcaccgtag gtcagggtca cggtatcgcc cgggcgcagc gtcccggagg caacgcgaaa
     5581 gacgagcgcg tcagccggac ggcggaaacc gccgtgcatg ccgatcatcg gggcggtttc
     5641 ggtggtgaaa ctcgcgtcgg cagcgctggt ggttattgac aggtagttgt ccgcggctgg
     5701 atcgcccgtc tgccacgcgc cgtagttggg ctggaaatgc ctggccacca gaatgcctcc
     5761 gcccgcctgt atcgccttgg aaccgacggt gaacgtctgc ctcagcgtga cgaaggacgc
     5821 tgcctggaag ggtcccgaat cggctcgaag ggttccaagc gccccgggct cctcgtcgag
     5881 cagggtcaac tcctggatga agccgtcgat gacgtcgtgc cgatcggcca tcaggtccgg
     5941 ataggcgaac acgaaagaga cggcctgggt caggttgacc ggcagcacac caccgtcgag
     6001 agacaaggca ttggcccagt tccaaagcac gcgggaccgc tccgcgacgt tggtggtgtt
     6061 ggtcggtacc tgctggacgt cggccttcag ccgttcgacg cgcgcgcgca gatcgggcgt
     6121 caggtaatcc gtggtgggct gcgcgaaagc cgacagggcg agcagcacga gcaggggcgc
     6181 ggtgaacgaa ctaaaggcgc gcaagcgtca gggctccacg gtcactggac cggggcgagc
     6241 ttctcacatg gcggcgtggg agggtagcgc aatcgccggc cgggcctcag accggcgagt
     6301 cgccggccac ggtggtgcgg tgcatgaccc ggcgataccc gtcgtatccg cccctggcgc
     6361 aatgctgaac cgagcggttg tcccacatgg tcagcatgtc gggcgcccag cggtgacggt
     6421 agacgaactc gtccctaatg gcgtgatcga acaaccgctt cagcagattc tttgcgtctt
     6481 cctccgccat gttccgaatg ccgatggtgt agacgccgtt cacccacagg gccttgcggc
     6541 cggtttcggg gtgcgtgcga acgagcggat ggtcctgggt ctgcagtgct tcgtcgctgg
     6601 gcaggatggt catgctccgc tccgggcctc tcccggccag gaatccctgg tggctgtagg
     6661 ggcggcgggc gctgtggatc gcggtgagct cggagatctc gtcgcgcagg gttgccggca
     6721 gggcctcgta agccgcgtag ccatccgcgt accaggtctc gccgcccacc ggggggacga
     6781 tcttggcgtg cagaatggtt gcggccggcg gctgggcctg gaaggaccag tcggagtgcc
     6841 aggacgcccc gaaaggggcg acgttctccc caggctcgcg cctgacttcc aggatgtgcg
     6901 gatgaccggg gatggctttc acgtaggggt cgtttccgaa agcgccgaag cacagagtaa
     6961 atcgttccag gtcctcgtgg ctcatgggct ggtcggggaa gaagagcacc tgatg
//
LOCUS       DBNHKV010000071         2129 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1003210, whole genome shotgun sequence.
ACCESSION   DBNHKV010000071 DBNHKV010000000
VERSION     DBNHKV010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1003210"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..1543)
                     /locus_tag="AC16PP_02020"
     CDS             complement(206..1543)
                     /locus_tag="AC16PP_02020"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6480429.1"
                     /translation="MAAIVDRSKGISYQELIAQDPVAPPKTLTLENAYQPGIATRVPV
                     SRYASKEFHDLEMEKLWPRVWQMACREEEIPEVGNYTIYEIGSFSIVVVRTNQGIKAH
                     HNVCRHRGRRLCDHPGHASSFICPFHGFSWNIDGSLRSVTSEWDFPHIDKDELTLTPV
                     RCETWAGWVFVNMDMNAEPLMDFLGDLPAHFAVWEPENRYIEAHVAKIMSCNWKACQE
                     AFMEAFHVINTHPQILVGIGDENTQYDVWGNFSRAITPNGTPSPHLRFEPDEQELFDS
                     VTMRYMDRPSLGEVPEGMTARAMMAAGARPAIEALVGEGKDLSDASLSDSIYYTLFPN
                     FHPWGGYNRIVYRFRPYKNHHDKSIMECYYLSPFAGERPEPAPVHWLGEDEDWTEATE
                     LGILAKVFQQDTFNLPKVQIGLEAAQYEEIVLANYQESKLRHFHTLLEEWIGR"
     gene            complement(1600..1836)
                     /locus_tag="AC16PP_02025"
     CDS             complement(1600..1836)
                     /locus_tag="AC16PP_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480430.1"
                     /translation="MPILKHPLSGAIYTARDDGLVEVDNNGLKGLFHFDGRHHSGELR
                     HADPHMLLWLAGPQLPADAAVRINRPAPVPPSEG"
     gene            complement(1874..>2129)
                     /locus_tag="AC16PP_02030"
     CDS             complement(1874..>2129)
                     /locus_tag="AC16PP_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480431.1"
                     /translation="EPIPGPVNLFQLVTHQALMQGYLTHLAEHRYPEARAAMTRWLKS
                     GELKNVEYRLQGIENVGRAFCDLFGGNNFGKTIVRLRDEQ"
BASE COUNT          364 a          655 c          685 g          425 t
ORIGIN      
        1 ggcgcggtga gcttggctgg ccaacctttc agcgtaaccc cgaaccagac ttcctcgtcg
       61 atctccacgg gcttcagaaa gagatcgaag ggcatgtaga tgaaggtcat gtaaagacag
      121 aagaaccaga tcaggttcat ccaccagggc cgtcgcgacc attcgatcat gaagttcatg
      181 acaaacaccg cgcctggtta cggatctacc gcccgatcca ctcctccagc agcgtgtgga
      241 agtggcgcag cttgctttcc tgatagttgg ccaggacgat ctcctcgtac tgggcggctt
      301 ccaggccgat ctgcaccttg ggcaggttga aggtgtcctg ctggaagact ttcgcgagga
      361 ttccgagttc ggtggcctcg gtccagtcct cgtcctcacc cagccagtgc acgggggccg
      421 gctccggccg ctcgccggcg aaggggctca ggtagtagca ctccatgatc gacttgtcgt
      481 ggtggttctt gtacggccgg aagcggtaga cgatccggtt gtagccgccc cacgggtgaa
      541 agttggggaa gagcgtgtag tagatgctgt ccgacagcga agcgtccgac aggtccttac
      601 cctcccccac gagggcctct atggcggggc gcgcaccggc cgccatcatg gcccgggcgg
      661 tcattccctc ggggacctcc cccagggagg gccgatccat gtaacgcatg gtcaccgagt
      721 cgaaaagctc ctgctcgtcc ggctcgaacc tcaagtgcgg gctgggcgtc ccgttgggcg
      781 tgatggcccg ggagaagttg ccccagacgt cgtactgggt gttctcgtcg ccgatcccca
      841 ccagaatctg cgggtgggta ttgatgacgt ggaaggcctc catgaaggct tcctgacacg
      901 ctttccagtt gcagctcatg attttggcca catgggcttc gatgtaccgg ttctccggct
      961 cccagacggc gaagtgcgcg ggcagatcgc ccaggaagtc catgagcggc tcggcgttca
     1021 tgtccatgtt gacgaacacc cagccggccc aggtctcgca acgcacgggc gtgagtgtca
     1081 gctcgtcctt gtctatgtgg gggaaatccc actcggaggt gaccgatcgc agcgatccgt
     1141 cgatgttcca gctgaacccg tggaagggac agatgaagct cgaggcgtgc cccggatgat
     1201 cacaaagccg tcggccccgg tgacggcaga cgttgtgatg tgccttgatg ccctggttgg
     1261 tacggaccac cacgatggag aagctgccga tctcgtagat ggtgtagtta ccgacctcgg
     1321 ggatctcctc ctcacggcag gccatctgcc agacccgggg ccacagcttc tccatctcca
     1381 gatcgtgaaa ttccttcgat gcgtagcggc tcacggggac gcgggtcgcg atgcccggct
     1441 ggtaggcgtt ctcgagggtc agggtcttcg gcggcgccac gggatcctgc gcgatgagct
     1501 cctggtagct gatgcctttc gatcggtcga cgatggcggc catttcgttc tcctggatat
     1561 gtcggttggt tcggtgtcgt ccgcgcccgg tgcgccggtt cagccctcgg agggcggcac
     1621 cggtgcgggg cggttgatgc gcacggccgc gtcagccggc agctggggcc cggccagcca
     1681 cagcagcatg tgcggatcgg cgtggcgcag ctcgccggaa tggtggcgac cgtcgaagtg
     1741 aaacaggcct ttgagcccgt tgttgtccac ctccacgagc ccgtcgtccc gggccgtgta
     1801 aatggctccc gacaggggat gcttcagaat cggcatggtc ttcttctcct cctctgcgta
     1861 tacaggtacc gcatcactgc tcgtcgcgca accgcacgat ggtcttgccg aaattgttcc
     1921 ccccgaacag atcgcagaac gcgcggccga cgttctcgat gccctgaagc cggtactcga
     1981 cgttcttgag ctcgcccgac ttcagccacc tcgtcatggc ggcccgggct tccggatagc
     2041 gatgctcggc gaggtgcgtg aggtagccct gcatgagcgc ctgatgggtc accagctgaa
     2101 agaggttgac cggccccggt atcggctcc
//
LOCUS       DBNHKV010000072         3857 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1641190, whole genome shotgun sequence.
ACCESSION   DBNHKV010000072 DBNHKV010000000
VERSION     DBNHKV010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1641190"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..992
                     /locus_tag="AC16PP_02035"
     CDS             114..992
                     /locus_tag="AC16PP_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR6480432.1"
                     /translation="MILSTDGGGSRGLIAANVLYHMEKKAEIPIRETFDFSAGVSTGA
                     MVAAYLARNAGTMEEVAKKAYSSENLSKIMNKSIWDRILGRLQNQPKYDGQNERAYVS
                     SVTQDSRINDITDKFLLILAYDFINRELVTFKNNRGHDGWYNPSLTEVCDAASAAPTL
                     REIWVVSLGTGRPMRELSQDERDRIGEASRDWGIVGWVANGLLDHMMSASSCVSTHQC
                     EQLLGDRYVRIGGELPRKLMQLDNTSETRVADLQSYAFLWFEEHIGRVQQFLEDCEVA
                     RRRWAEEAKANGEYAV"
     gene            1023..2252
                     /locus_tag="AC16PP_02040"
     CDS             1023..2252
                     /locus_tag="AC16PP_02040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6480433.1"
                     /translation="MSRDSSEVRRNWQLLVSACTGIICSSIVLPYYSIGALVVPITEE
                     FGWQRSQFQMAILFSSGLGALTAPVVGWLIDRYGARRIALPGILGLSVGFVLATFMDG
                     RLWMLYLSYGAMALLGAGTIPVTWTRAITTNFFRQRGLALGLTLSGTGLCAVIVPQYA
                     VWMVEAYGWRAAYLGLAAMPLLIAGPVVYFGFRPREGIGGEALETGEAQWGMTLGEAF
                     RTTKYWVLLISIFVVYMAVSGIGPNLIPALVDDGLTPAQAATALSTFGGAIIAGRIIV
                     GYLVDRFWAPGVAALSLSLPVAGCLMLVGAPGFETAVAAALLIGIAAGAELDLMSFLA
                     AKYFGLRHYAKIYAVLYATLAVCSGTAPMLFARVYDVTASYDVSFWISTVLFALGAVL
                     VLAMGAYPRMGDSRELP"
     gene            complement(2306..3424)
                     /locus_tag="AC16PP_02045"
     CDS             complement(2306..3424)
                     /locus_tag="AC16PP_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480434.1"
                     /translation="MQIRTQAAAVLIVLLAGGCAVQQPVQPTGIANITRDQIGRMAVR
                     SPDRPKVALAANVDTKGAAARKTALSAGATWLGGSFEAAGQAGEGGLLIAAFGLVTAP
                     IVAAGGAVYGAAAADSKEEITSGNEVLVRTLDFAPAQMQRALENELTTSAPMDWEFVD
                     PDASNAELAARGFDSVLDMDMDSIASHVSANDFHVYFDTLNRLTLTSLVSGQSLAIRK
                     YWRELPPRAVSDWAGDGGSVLTNALHEQFADIAREMADDLFLYPAIRVHGVEPVSRRR
                     FGAGTISGRRPMFVWSALDGGNGTPAQAVEYELAIAADRQANPVSFRTHKMRYVPPDS
                     LEACRTYSWKVRAHYLSFGEPTTSQWSPEYRFKTPCRK"
     gene            complement(3604..>3857)
                     /locus_tag="AC16PP_02050"
     CDS             complement(3604..>3857)
                     /locus_tag="AC16PP_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480435.1"
                     /translation="LPMLLYLKDRIAEAQDAFADLLKHHPRVNPRNRLTYGWFRPIEP
                     LIREHVAQASGTLRKERSLADLLEWIFLDLGWEPPTPMP"
BASE COUNT          616 a         1185 c         1292 g          764 t
ORIGIN      
        1 acgtcgtcat gcttccccct gcggcatttt cccggcgttc gttgtacggt acgttagcgg
       61 ctaatggcta caatgggatc aagaggggcg gtatgcaaag caaaggagca atcttgattc
      121 tctccaccga cggtggcggt tcccggggtc tgatcgcggc gaacgtgctc taccacatgg
      181 aaaagaaggc cgagatcccg atacgtgaaa cgttcgactt ctccgctggc gtgagcactg
      241 gtgccatggt ggctgcctac ctcgcacgca acgcgggcac catggaagag gtggccaaga
      301 aagcctactc ctccgagaac ctgtccaaga ttatgaacaa gtccatctgg gaccggattc
      361 ttggcaggct tcagaaccag ccgaagtacg acgggcagaa cgagagggca tacgtctcgt
      421 cggtgaccca ggattcccgg atcaacgaca tcaccgacaa gtttctgctg atcctggcct
      481 acgacttcat caatcgggag ctggtcactt tcaagaacaa ccggggccac gacgggtggt
      541 acaacccgtc cctgacggag gtttgcgatg cggcgtccgc ggcgcccacc ctcagagaga
      601 tttgggtggt gtcgctgggc accggcaggc cgatgcgcga gctcagccag gacgaacgcg
      661 accgcatcgg cgaggcgtcg cgggattggg gaatcgttgg ttgggtggcc aacggtctac
      721 tcgatcacat gatgagcgcc agctcgtgcg tcagcaccca tcagtgcgaa cagctgctcg
      781 gcgatcgcta cgtgcgcatc ggcggggagc tgcctcgcaa gctcatgcag ctggacaaca
      841 cgtcggagac tcgggtggcc gatcttcaat cctacgcttt tctctggttc gaggagcaca
      901 tcggtcgcgt tcagcagttc ctagaggatt gcgaggtagc ccggcgccgg tgggccgagg
      961 aagccaaagc caacggcgaa tatgcggtct gagccgggtt cgcccaggcg gcgccgatcg
     1021 gcatgagcag ggattcgagc gaggttcggc gcaactggca gctgctggtg agcgcgtgca
     1081 cgggcatcat ctgcagctcc atcgttctac cgtactactc catcggcgcc ctggtggtgc
     1141 ccattacgga ggagttcggc tggcagcgct cccagtttca gatggccatc ctcttcagct
     1201 ccggcctggg cgcgctcacc gcgcccgtgg ttggctggct catcgatcgc tacggggctc
     1261 ggcgcatcgc gttgccgggg atcctcgggc tgtcggtcgg cttcgttctg gcgacgttca
     1321 tggacgggcg cctgtggatg ctctatctgt cttatggcgc catggccctg ctcggcgcgg
     1381 ggacgattcc ggtcacctgg acccgtgcca ttaccaccaa cttctttcga caacggggcc
     1441 ttgccctggg gcttacgctc agcggcacgg ggctgtgcgc ggtgatcgtt cctcagtatg
     1501 ccgtctggat ggtggaagct tacggctggc gcgccgcgta cctcgggctc gcggccatgc
     1561 cgctgctgat cgccgggccc gtggtgtact tcggctttcg cccccgggag gggattggcg
     1621 gagaggccct ggagaccggc gaggctcagt ggggtatgac cctgggagaa gctttccgga
     1681 ctaccaagta ctgggtgctg ctgatctcca tcttcgtggt ttacatggcg gtttccggca
     1741 tcggccccaa cctcatcccg gcgctggtgg acgacgggct gacgcctgcc caggcggcca
     1801 cggcgctcag cacgttcggc ggcgcgatca ttgccgggcg gatcatcgtg ggttacctcg
     1861 tggaccggtt ctgggcgccg ggagtggcgg ccctgtccct cagccttccc gtggccggct
     1921 gcctgatgct ggtgggcgcc cccggcttcg agaccgccgt ggccgccgcg ctgctgatcg
     1981 ggatcgcggc gggtgcagag cttgatctga tgtcgtttct ggcggcgaaa tacttcggtc
     2041 tgcgtcacta cgccaagatt tacgcggtgc tatatgccac cctggccgtc tgctcgggca
     2101 cggctcccat gctgtttgcc cgggtgtacg acgtgacggc cagctacgac gtgagcttct
     2161 ggatctccac ggttctgttc gcgctgggcg ccgtgctcgt cctagccatg ggcgcgtatc
     2221 cgcggatggg ggattcacgg gaactgccct gatccgctcc gatgcagggc gcgtagcgtg
     2281 gtgtcagggc gagggttggc gacatctact ttctgcacgg tgtcttgaac cgatactccg
     2341 ggctccattg gctcgtggtc ggctcgccga agctcaggta gtgggcgcga accttccagc
     2401 tgtaggttcg acaggcctcc agggagtccg ggggcacgta gcgcattttg tgggttcgaa
     2461 agctcaccgg attggcctgt cggtcggccg cgatggcgag ctcgtactcg acagcttggg
     2521 cgggtgtgcc gttcccgccg tccagagccg accagacgaa catgggccgc cgcccggaga
     2581 tcgtgccagc gccgaatcgt cggcgcgaga cgggctcgac gccgtggacc cggatggcgg
     2641 ggtagagaaa gaggtcgtcc gccatctctc tggcgatgtc ggcaaactgc tcgtgcaggg
     2701 cattggtcag gacgctgccg ccgtcaccgg cccaatcgga aaccgcccgg ggcggaagct
     2761 cccgccagta ctttctgatg gcgagcgact ggccgcttac gaggctggtc agggtcagac
     2821 gattcagggt gtcgaaatag acgtggaagt cgtttgcgct cacgtggctg gcgatgctgt
     2881 ccatgtccat gtccagcacg gagtcgaagc cgcgcgcggc gagctccgcg ttgctggcat
     2941 cggggtcgac gaattcccaa tccatgggcg ccgacgtcgt caactcgttc tccagtgccc
     3001 gctgcatctg cgcgggcgcg aagtccagcg tccgaacgag cacctcgttt ccggaagtga
     3061 tctcttcctt ggaatcagcg gctgcggccc cgtagacggc ccctccggcg gcgacgatgg
     3121 gggcggtgac gagcccgaag gcggcgatca gcaatcctcc ctcaccggct tgacctgccg
     3181 cttcgaagga tccgcccagc caggttgccc ccgccgagag cgccgttttg cgtgctgccg
     3241 cgcccttcgt gtccacgttc gccgccagcg ccaccttggg acgatccggg ctgcgcacgg
     3301 ccatgcggcc gatctggtcc cgggtgatgt tcgcgatccc cgtgggctga acgggttgct
     3361 ggaccgcgca gcctccggcc agaagcacga tgaggacggc ggcggcttgg gttcggatct
     3421 gcatctggct gtctccctgc gtgtacgtgg caggcagcct cgcatcgccg cgcgcgcaga
     3481 tcctgatgtg cggctgaagc ttgttgtggt tttcttaggc gccggtaact atttgattta
     3541 tttatattta aatttggcct tgcgcccgta aacacgtgga cgtcgaagcg ccacgtcgag
     3601 ccgtcagggc atcggggttg gcggttccca gcccagatcc agaaagatcc attccagcag
     3661 gtcggccaac gaccgctcct ttcgcagcgt gccggacgcc tgggccacgt gctcgcggat
     3721 caggggctcg atgggccgga accaaccata ggtgagccgg ttgcggggat tcaccctggg
     3781 gtgatgcttc aacagatcgg cgaaggcatc ctgagcctca gcaatgcggt ctttcaggta
     3841 gagcagcatc ggcagct
//
LOCUS       DBNHKV010000073        12726 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1216106, whole genome shotgun sequence.
ACCESSION   DBNHKV010000073 DBNHKV010000000
VERSION     DBNHKV010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12726
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1216106"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="AC16PP_02055"
     CDS             <1..252
                     /locus_tag="AC16PP_02055"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014033.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HIR6480436.1"
                     /translation="LPPLTEFVIPGGTVAAALAHVCRTTCRRAERELWALQDDDSATA
                     AARYTNRLSDLLFVMARTLNAAEGAEPQWRGTRGPKGPE"
     gene            complement(271..1953)
                     /locus_tag="AC16PP_02060"
     CDS             complement(271..1953)
                     /locus_tag="AC16PP_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HIR6480437.1"
                     /translation="MATEIRIPDIGDVEHVEVVEICVAAGAEVAADDALIIIESDKAS
                     MEVPAGVAGVVASLAVAVGDTVAEGDLIAVIEAAGAAPVEDQAGEVAAEAPPQMQDPK
                     VPPERAVASPPTKPEAGPSGTVEIRVPDIGEAEDVVVVEVAAAAGQQVGEDDLLVVVE
                     SDKASMEIPAGCSGTVLEMHVSEGAAVTEGTLIATVEVKGSSAAPAPAADRPEVEAGE
                     VPRGAEAAAPQPISTTAAPPAGPDTAAAEQESAGTALGERIYAGPAVRRLARELGVDL
                     ARVRGTGARERIVKDDVKAYVKDAMTAAPGGAGAGAASGAGIPAIPQIDFSRFGPVEI
                     RPLTRIRAAGAANLQRSWLNLPHVTQHDEADVTELEAFRASLKSEAERRGVKITPLAF
                     IIKACCYALKKFPTFNASLDAEARNYVLKGYYNIGFAVDTEEGLVVPVIKGADQMGIW
                     ELSEAVTDLAGRAREQKLGMNDMQGGSFSVSSLGAIGGTGFTPIINAPEVGILGVSRL
                     AMRPVWDGESFVPRKLLPLSLSYDHRAINGAEAGRFVSHLCELLADIRRLSL"
     gene            complement(1966..4629)
                     /gene="aceE"
                     /locus_tag="AC16PP_02065"
     CDS             complement(1966..4629)
                     /gene="aceE"
                     /locus_tag="AC16PP_02065"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014603513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIR6480438.1"
                     /translation="MAYPRDLRDEDAAETREWLDSLEYVLENAGVDRANFLLERLSAR
                     MTRTGARLPYTITTPYRNTIPSSREAVMPGDLFMERRIRSLIRWNALAMVVRANRRPG
                     DLGGHISSFASSATLYDVGFNYFFRGPTDEHPGDLIYFPGHASPGIYARSFLEGRLSE
                     AQLDNFRREVAGEGLSSYPHPWLMPDYWQFPTVSMGLGPIQAIYQAHIMKYLDARGLL
                     PMGERKVWAFLGDGECDEPEALGALSKAGTEKLDNLIFVVNCNLQRLDGPVRGNSKII
                     QELEGNFRGAGWNVIKVVWGRLWDPLFANDTAGLMQLRMNEVVDGEYQNYKAKGGAYT
                     REHFFGAHPELLAMVERLSDDDIMRLNRGGHDPFKVYAAYHEAVHHKDEPTVILAKTV
                     KGYGMGDAGEAENDTHQVKKLNLDELKRFRDRFDVPLSDTELEDIPFYRPPKDSPEMV
                     YLRKKRADLGGPIPQRTTASEALQVPELSAFAQQLQGTGDRSNSTTMAFVRMLGTLVR
                     DKNIGKRIVPIVPDEARTFGMEGMFRQLGIYSSAGQLYEPEDYGEIAAYRESRDGQVL
                     EEGINEAGAFSAWLAAATSYSSHRFTLIPFYIYYSMFGFQRVGDLAWAAGDIQARGFL
                     LGGTSGRTTLNGEGLQHQDGHSHVLASTIPNCVTYDPCYAYELAVIVHDGLKRMVENG
                     ENVFFYITVMNESYRQPAMPEGVEEGILRGIYRVRTVGDGETRRVRLLGSGTILREVE
                     AAADMLHEDYGVSAEVFSVTSYNELARDGQDVERWNLLHPEEPPRVSYVSQQLAGDDP
                     VIASSDYMKSLAEQVRPYIGAPYKVLGTDGFGRSDTRESLRHFFEVDRKFVALAALRS
                     LADAQKIPLEEVRQARDAMGIDADKPNPRTA"
     gene            complement(4664..5638)
                     /locus_tag="AC16PP_02070"
     CDS             complement(4664..5638)
                     /locus_tag="AC16PP_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR6480439.1"
                     /translation="MKRIGIDLGGTKIKGIVLAEDGRVLSTRRAATPSQSYAGTVAAI
                     VRMVAELDGDSGEARDLPVGIGTPGIWMAQRSAMKNCNSTWLNGQPLLLDLEHALGAR
                     VRIANDADCFALSEAMAGTGRGYGSVFGVILGTGVGGGFVTGGRLMSGPNGLAGEWGH
                     TPLPYLGLHEALEISVGSELDRRHEQANALESRLAVRTCYCGRPGCVETYLSGPGWRQ
                     THRELWSEDVPTETLAKRRDERSEQTFFLYTRMLARSLAQIVNIVDPAVIVLGGGLSN
                     VQALYQPVNEMLARYVFSGECDTQVKAPQLGAESGVLGAAQLWSDNGG"
     gene            complement(5635..6186)
                     /gene="ampD"
                     /locus_tag="AC16PP_02075"
     CDS             complement(5635..6186)
                     /gene="ampD"
                     /locus_tag="AC16PP_02075"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HIR6480440.1"
                     /translation="MRVRKDHCLVGVPFRASPNCDERPDPEDVSLIVIHGISLPPGRF
                     GGDLVDGLFTNSLPDELDPGLAELRDVRVSAHLLIDRAGRITQYVPFHRRAWHAGSSG
                     WCGRPSCNDYAIGIELEGTDELDYADAQYRALIAVSRALFDRYPRLSVDAVVGHQEIA
                     PERKTDPGPAFDWCRYLGALAGP"
     gene            6273..7130
                     /gene="nadC"
                     /locus_tag="AC16PP_02080"
     CDS             6273..7130
                     /gene="nadC"
                     /locus_tag="AC16PP_02080"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR6480441.1"
                     /translation="MAPDGFPHADELADAVGESVRAALAEDVGTGDITAELIAPDARA
                     TAEVITRDEGIFCGAPWVLETCAQVDAAIEVTLHCADGDRVSPGQTLFALSGPARGLL
                     TAERTMLNFVQLLSGTATATRRYVDLIAGTAARLLDTRKTVPGLRMAQKYAVHCGGGH
                     NHRIGLFDAFLIKENHLAAAGGIAPAVARARELYPDRPVEVEVESLDELDQALAGGAD
                     IALIDNFSLEDTSAAVTLAAGRIELEASGGIDEATITEIARAGVDYISLGVITKRVIP
                     LDLSMRFTS"
     gene            7136..7873
                     /locus_tag="AC16PP_02085"
     CDS             7136..7873
                     /locus_tag="AC16PP_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480442.1"
                     /translation="MTRISAFLIHLGISFAIFLGLAYLVVFIWYPDFFFSTDGGWQGI
                     RIIVAVDLVLGPALTLIVFKPGKPGLKFDLTLIGLFQAACLIAGVYVVHSERPLAMVY
                     VDGQFFSMSGDDYSGVGLPIPDLRAVPGKSPKWVTVEIPHDPWISQEIRMSAMRKGTP
                     LRTLADRYAPFSVEDVDLEREAYPLSELVDRDQLTQQIPLWLDEHGGTLEDYAFVRLA
                     TRYRYVFLGIDRRDGAIAGVLGTPAPL"
     gene            complement(7880..9742)
                     /locus_tag="AC16PP_02090"
     CDS             complement(7880..9742)
                     /locus_tag="AC16PP_02090"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR6480443.1"
                     /translation="MFYGSDVNVVVFFGAIFVLMSGLSLVAPRLATEVHASPVTFALP
                     ALAIVGIVVSYQFSLSKDSSFVPAWVVAVTPLAFLLARGAGEAAFALHKAVSLTVLAF
                     AAISVVLLFSAGVRARLPLTDPNNYATLLCLILLPWLHRYFVLRTGSQGMSLRVEGKD
                     LSACFILALALFATGSRAGLLVIAGAFIIWAGLAIARRLRWRPVAAAAALTAAAYLLA
                     GASLTDQSVALTAQAAAADRVAGADIEVRMALIGSALAMYAEYPATGIGLFVFPLLYR
                     FQRPVVDQDSAGLYVHNDYLQLLLEGGPLLFCAMVTFAVVVVIVFFRRLAALPGDEAF
                     ARLGLTMGVGAALAHALVNFVVYSPALGFVLGLTVAASFRDSKGSGSVDSTQARPIRV
                     GVTLAVLFGWTAWLYLGLDTATAGILQNQRYVPFTEEVRKSPERMLRFAGMARSLNAD
                     RGVPLLAEAVLLDNKLRQNPESRYLGEETLNAFRRAGRADPWNTLTLLLMADFIMRNP
                     SVLPLLENGEAPEELLLRTLVMDPQFVPGIDALMDKLGDEGRHAEAEALLRLRIYPWL
                     RLLARQNKPAAARYAEALLGMARARGDSEFVSELEARQQEVENTQPVIAQRWFF"
     gene            complement(9850..9925)
                     /locus_tag="AC16PP_02095"
     tRNA            complement(9850..9925)
                     /locus_tag="AC16PP_02095"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9890..9892),aa:Thr,seq:cgt)
     gene            complement(10004..10387)
                     /locus_tag="AC16PP_02100"
     CDS             complement(10004..10387)
                     /locus_tag="AC16PP_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003161762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR6480444.1"
                     /translation="MAMIKGRASHGFSLIELMIAVAIIAIIAAVALPLYQGYIQTSRE
                     GVLVNNISTIEIFQEDRMLRTGSYLLTAANTAAITAGIGWTPRDDGELSYSIADGGGG
                     SYDVTATDDTGVTVCMRFPQKVRCP"
     gene            complement(10470..10940)
                     /locus_tag="AC16PP_02105"
     CDS             complement(10470..10940)
                     /locus_tag="AC16PP_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HIR6480445.1"
                     /translation="MKNAMQKGFTLIELMIVVAIIGILAAVALPAYQDYIENSNMAKV
                     TSHFEEGSRLVANEMRKVQADVAIGRTLNLAAADALPQYANAAGWAGLLNSNGGTAPG
                     GGLPYNVAPDDANGIVGIAKTGTFAAGDLTIVVTRPNYGGFSGQSASTILFNSI"
     gene            11412..>12726
                     /locus_tag="AC16PP_02110"
     CDS             11412..>12726
                     /locus_tag="AC16PP_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HIR6480446.1"
                     /translation="MASDPIPLSGLARRLVDDQIIDFDSANKASQEARKQGSQFVTFL
                     VENAMADARTIAVSASREFGVPLMDLSSFDLEAIPRDLVKDSLIKQHSALPLVRRGAR
                     LFVALSDPTNLQALDEIKFQTGVNTETVLVEDDKLQVLIDQFLTAVEDDGLGDMANDD
                     LDDLDLEASGDSQDDDEDDVTSGTDSRPIVRFVNKMLLDAIKTGASDIHFEPYERSYR
                     VRFRTDGMLNEISKPPVNLGTRLAARLKVMSELDISERRVPQDGRIKMRLSKTRTIDF
                     RVNTLPTLWGEKIVLRILDPSSAKMGIEHLGFEPDQEKILVGTLHQPQGMILVTGPTG
                     SGKTVTLYTGLNILNTMERNIATAEDPVEINLEGINQVNVNYKVGLDFASALRSFLRQ
                     DPDVVMVGEIRDLETAEIAIKAAQTGHMVLSTLHTTSAPETLTRLR"
BASE COUNT         2371 a         4250 c         3908 g         2197 t
ORIGIN      
        1 ctgccgcccc ttaccgagtt cgtcatcccc ggcggcacgg tcgcggccgc tctggcccac
       61 gtctgcagga cgacgtgccg ccgcgccgag cgcgagctgt gggccttgca ggacgacgat
      121 tcggcgacag cggccgcccg ctataccaac cggctgagcg atctgctgtt cgtgatggcc
      181 cgcaccctga acgccgccga gggagccgag cctcagtggc gcggcacgcg cggcccgaag
      241 ggcccggagt agcgcagggg cccgaaaggc ctagagagaa agccggcgga tgtcggccag
      301 cagctcgcag agatggctga cgaacctgcc cgcctcggcg ccgttgattg cccgatggtc
      361 gtagctcaac gacagcggga gcaattttct cggcacgaag ctctcgccgt cccagaccgg
      421 ccgcatggcg agccgggaca cacccaggat gcccacctcc ggggcattga tgatgggcgt
      481 gaagccggtt ccaccaatgg ctcccaggct cgaaacgctg aagctcccgc cctgcatgtc
      541 gttcatgccc agcttctgct cgcgggccct gcccgcgagg tcggtaacag cctcggagag
      601 ctcccagatg cccatctgat ccgcgccttt gataaccgga acgaccaggc cctcctcggt
      661 gtcgacggcg aagccgatgt tgtaataacc ctttagaacg tagttgcggg cttcagcgtc
      721 cagggaggcg ttgaaggtcg ggaacttctt cagcgcgtag cagcaggcct tgatgatgaa
      781 tgccagcggc gtgatcttca cgccccgccg ctccgcctcg ctcttcagag aggcgcgaaa
      841 ggcctcgagc tccgtgacgt ccgcctcgtc gtgctgggtg acgtggggca ggttcaacca
      901 gctgcgctgc aggttggcgg cgcccgccgc gcgaatccgc gtcagcggcc ggatctccac
      961 cgggccgaat ctggaaaagt cgatctgcgg tatggccggg atgcccgctc cgctcgcggc
     1021 ccctgctccg gcaccgcccg gcgccgcggt catggcatcc ttgacgtacg ccttgacgtc
     1081 atccttcacg atgcgctccc gcgcgccggt gcccctcacc cgggcgagat ccacccccag
     1141 ctcacgggcg agccggcgca cggctgggcc ggcgtagatg cgttcgccca gagccgtccc
     1201 ggccgactct tgctccgccg ccgcagtgtc cgggccggcc gggggcgcag ccgtcgttga
     1261 gataggctgc ggggccgctg cttcggcgcc cctgggaacc tcgcctgctt cgacctcggg
     1321 ccgatctgcc gcaggtgcgg gggccgccga cgatccctta acctcgaccg tggcgatgag
     1381 ggtcccttcg gtaactgccg caccttccga tacgtgcatt tccagaaccg ttccggaaca
     1441 ccctgccggt atctccatgg acgccttgtc ggactccacc acgaccagaa gatcgtcctc
     1501 gccaacctgc tgcccggcgg cggctgcaac ctccaccacc acgacgtcct cggcctcgcc
     1561 gatgtccggc acccggatct cgacggtacc ggacgggcca gcctcgggct ttgtcggcgg
     1621 cgaggcgacg gcccgttccg ggggcacttt cggatcctgc atttgcgggg gcgcctcagc
     1681 ggccacctcg ccggcctgat cctcgaccgg cgccgccccg gcagcctcta tgacggcaat
     1741 gagatcgccc tcggccacgg tgtcgcccac ggccaccgcc agggaagcca ccacgcccgc
     1801 cacccctgcc ggcacctcca tggacgcctt gtcggactcg atgatgatga gcgcgtcgtc
     1861 cgcggccacc tcggcacccg ctgcgacgca gatctccacc acctccacgt gctccacatc
     1921 gccgatgtcg ggaatgcgta tctctgtggc caactccagg ctccctcagg ctgtccgcgg
     1981 attgggtttg tcggcatcga tgcccatggc atccctggcc tggcgaacct cttcgagcgg
     2041 gatcttctgg gcatccgcca ggctgcgcag cgcggccagc gcgacgaact tgcggtcgac
     2101 ctcgaagaag tgtctgagcg attccctggt atcgctgcgt ccgaacccat ccgtgccgag
     2161 caccttgtac ggcgccccga tgtagggccg gacctgctcg gccagggact tcatgtaatc
     2221 gctggaggcg atcaccggat cgtctccggc gagctgctgc gagacgtagc tcacccgcgg
     2281 cggctcttcc ggatgcagca ggttccaacg ctccacgtcc tggccgtcgc gagccagctc
     2341 gttgtagctc gtgacgctga acacctcggc gctgacgccg tagtcctcat gcagcatatc
     2401 ggcagccgct tcgacctcgc gcagtatggt tccgctgccg agcaggcgta cccgccgcgt
     2461 ttcgccatcg ccaaccgtgc gcacccggta gatgccccgc aggatgccct cctccacccc
     2521 ttcgggcatg gccggctgcc gatagctctc gttcatcacc gtgatgtaga agaaaacgtt
     2581 ctcgccgttc tcgaccatcc gcttcagacc atcgtgaacg atcacggcca gctcgtacgc
     2641 gtagcagggg tcataggtga cgcagttggg gatcgtcgag gcgagcacgt ggctgtgacc
     2701 gtcctgatgc tgcagcccct cgccgttgag cgtcgtccgt cccgacgtac ccccgagcag
     2761 aaaaccgcgc gcctgaatgt ccccagcggc ccaggccaga tcgcccaccc gctggaaacc
     2821 gaacatggaa tagtagatgt agaagggaat cagcgtgaag cggtgcgagc tgtaggacgt
     2881 ggcggccgcc aaccaggccg agaaagcgcc ggcttcgttg atgccctcct cgagcacctg
     2941 gccgtcccgg gactcgcgat aggcggcaat ctctccgtaa tcctcgggct catatagctg
     3001 cccggccgag gagtagatgc ccagctggcg gaacatgccc tccatcccga acgtgcgggc
     3061 ctcgtcggga acgatgggaa cgattcgctt gccgatgttc ttgtcgcgaa ccagggtccc
     3121 caacatgcgc acgaacgcca tggtggtcga gttggaccgg tctccggttc cctgcagctg
     3181 ctgcgcgaag gcgctgagct ccggaacctg cagcgcttcc gatgccgtcg tgcgctgggg
     3241 aatgggaccg ccgaggtcgg cccgtttctt gcgcaggtac accatctccg ggctgtcctt
     3301 tggaggacgg tagaacggga tgtcctccag ctccgtatca gaaagcggga cgtcgaagcg
     3361 atcgcggaaa cgcttgagct cgtccaggtt gagcttcttc acctggtggg tgtcgttttc
     3421 cgcctcgcca gcgtcgccca tgccgtatcc cttgacggtc ttcgccagga tcaccgtagg
     3481 ctcgtctttg tgatgcacgg cctcgtgata ggccgcgtac accttgaagg ggtcgtggcc
     3541 gccccggttg agccgcatga tgtcatcgtc cgagaggcgc tcgaccatgg ccaggagctc
     3601 cggatgcgcg ccgaagaagt gttcgcgggt gtaggccccg cccttggctt tgtaattctg
     3661 gtactcgccg tccaccacct cgttcatgcg cagctgcatc aggcccgcgg tgtcgttggc
     3721 gaacagcgga tcccagagcc gaccccacac caccttgatc acgttccagc ccgcgccgcg
     3781 gaagttacct tccagctcct ggatgatctt gctgttgccc cgcacaggac cgtccagccg
     3841 ctgcaggttg cagttgacga cgaagatcag attgtcgagc ttctcggtgc cggccttgga
     3901 cagggcgccg agcgcttcgg gctcgtcgca ctcgccgtcg ccgagaaagg cccacacctt
     3961 gcgctcgccc atgggcagaa gcccccgggc atccaggtac ttcatgatgt gcgcctggta
     4021 gatggcctgg atcggcccca gccccatgga gaccgtggga aactgccagt agtcgggcat
     4081 cagccagggg tgcggatagg atgagagccc ctcccctgcc acttcccggc gaaagttgtc
     4141 cagctgggcc tcgctcaggc ggccctcgag aaacgaccgg gcgtatatgc ccggcgaggc
     4201 gtgccccgga aagtagatca ggtcgcccgg gtgctcgtcc gtcgggcccc ggaagaaata
     4261 gttgaagccc acgtcgtaaa gcgtggccga cgaggcgaag ctggagatgt gcccgcccag
     4321 atcgcccgga cgccggttcg cccgcaccac catggcaagg gcgttccagc ggatcagcga
     4381 gcggatgcgc cgttccatga agagatcgcc cggcatcacg gcttcgcggc tcgagggaat
     4441 ggtgttccgg taaggcgtgg tgatggtgta gggcaaacgc gcgccggtgc gcgtcatccg
     4501 ggcagacaaa cgctccagca gaaagttggc acggtccacg cccgcgttct cgagaacgta
     4561 ctcgagagaa tccagccact cccgggtctc cgccgcgtcc tcgtcgcgaa gatctcttgg
     4621 atacgccata tcagttcccg ctggccggct agccggccgg acctcaacct ccgttatcgc
     4681 tccacagctg tgcagccccg agcaccccgc tctccgcgcc cagctgaggc gctttcacct
     4741 gcgtgtcgca ctcgcccgaa aagacgtagc gcgcgagcat ctcgttgacc ggctgataca
     4801 gcgcctgcac gttcgacaac ccaccgccca gcacgatcac cgccgggtca acgatgttca
     4861 cgatctgagc caggctgcgc gcgagcattc gggtgtacag gaaaaacgtc tgctccgagc
     4921 gctcgtcgcg acgcttggca agcgtctccg tggggacgtc ctcgctccaa agctcgcggt
     4981 gcgtctgccg ccacccgggg ccgctgaggt aggtttccac gcagcccggc cgtccgcagt
     5041 agcaggttct gaccgccagc cgagattcta gcgcattcgc ctgctcgtgc cggcgatcca
     5101 gttcgcttcc caccgagatt tccagcgctt cgtgcaggcc cagataaggc agcggcgtgt
     5161 gcccccactc gccggccagc ccattcggcc cgctcatcag ccgtcccccg gtcacgaagc
     5221 cgccacccac gccggtgccc aggatgacgc cgaagacgct cccatatccc cggcccgttc
     5281 cggccatggc ttccgagaga gcaaaacagt cggcgtcgtt ggcgatgcgt acgcgggccc
     5341 cgagcgcatg ctccagatcg agcagcagcg gttgcccgtt gagccaggtg gagttgcagt
     5401 tcttcatcgc gctccgctgc gccatccaga tgccgggggt gccgatgccc acgggcagat
     5461 cacgcgcctc gccggagtcg ccgtcgagct cggcaaccat gcgcacgatg gccgcaacgg
     5521 tgccggcgta gctttgcgac ggcgtcgccg cccgtcgcgt cgacagcacc cggccgtcct
     5581 ccgccagcac gatgcccttg atcttggtgc cgcccaggtc gatgccgatg cgtttcacgg
     5641 gcccgccagc gcgccgaggt aacggcacca gtcgaaggcg gggcccggat ccgtcttgcg
     5701 ctccggcgcg atttcctggt gcccaacaac ggcgtccacg gacagccgcg ggtagcgatc
     5761 gaacagggcc cggctcaccg caatcagcgc ccggtactgc gcgtcggcat agtccagctc
     5821 atcggtccct tcgagctcga tgccgatggc gtagtcgttg cacgagggcc ggccgcacca
     5881 accggatgat cccgcgtgcc atgcgcgccg gtggaaggga acgtactggg tgattcggcc
     5941 ggcgcgatcg atgagcaggt gggccgaaac gcgcacgtca cgaagctctg ccagcccggg
     6001 gtccagctca tcgggcaggg agttggtgaa gaggccgtcg acgagatcgc cgccgaaacg
     6061 acccggcggc agggatatcc cgtggatcac gatcagcgag acgtcctcgg gatcgggacg
     6121 ctcgtcgcag ttgggcgagg cccggaacgg aaccccgacg aggcaatggt ctttcctgac
     6181 gcgcaaaacc tgtgatccgt gaccggcact gaagcagatt ctattgaagt agaatggccc
     6241 gcgcacaact atcggatatc ggaggcagcc cgatggcgcc cgacgggttc ccacacgccg
     6301 acgagctggc agacgccgtg ggcgaaagcg tgcgcgcggc tcttgccgag gacgtgggca
     6361 ccggggacat caccgcggag ctgatcgcac ctgacgcccg ggcaaccgca gaggttatca
     6421 cccgggacga gggcattttc tgcggggcgc cctgggtgct cgaaacctgc gcccaggtgg
     6481 acgccgccat cgaggtgacc cttcactgcg cggacggcga tcgcgtctcg ccggggcaga
     6541 cgctctttgc tctgagcggc cccgcgaggg gtctgctcac ggccgaacgg accatgctga
     6601 atttcgttca gctgttgtca gggacggcca cggcgacgcg gcgctacgtc gatctgatcg
     6661 cgggcacggc ggctcggctg ctcgacaccc gcaagacggt tcccgggctg cgcatggcgc
     6721 agaagtacgc cgtgcactgc ggcggcgggc acaatcaccg gatcgggctc ttcgacgctt
     6781 ttctgatcaa agagaatcat ctggctgccg ccggcggtat cgcgcccgcc gtggccagag
     6841 cccgcgagct ctacccggac cggcccgtgg aagtggaggt ggagtccctg gacgagctcg
     6901 accaggccct cgccggcggg gccgacatcg cgctcatcga caatttttcg ctcgaggata
     6961 cgtctgccgc cgttacgctg gctgcaggac gcatagagct cgaggcttcc ggcggtatcg
     7021 atgaggccac gatcacggaa atcgcccgcg ccggcgtgga ctatatctcc ctcggcgtta
     7081 tcaccaagag agtcattccg ctcgacctgt ccatgcgctt cacgagctga cgatcatgac
     7141 ccgtatcagc gcctttctga tccatctcgg aatcagcttc gcgatctttt tagggcttgc
     7201 ctatctggtc gttttcatct ggtaccccga cttcttcttc agcaccgacg gcggctggca
     7261 ggggattcgc atcatcgtcg ccgtggatct ggtgctcggt cccgcgctga cgctgatcgt
     7321 cttcaagccc ggcaagccgg gactgaaatt cgatctgacc ctgatcggac tgtttcaggc
     7381 agcgtgcctg atcgccggtg tgtacgtggt ccactccgag agaccgctgg ccatggtcta
     7441 tgtcgacggg cagttcttca gcatgagcgg cgacgactac tccggggtcg gattgcccat
     7501 acccgacctg cgggccgttc cggggaagtc acccaagtgg gtcacggtgg aaattccaca
     7561 cgacccgtgg atctcgcagg aaatacgcat gagcgccatg aggaagggaa cacctctgcg
     7621 cacgcttgcc gatcggtacg caccgttttc cgtagaggat gtcgacctgg agagggaagc
     7681 gtatcccctg agcgaactcg tcgaccgcga tcagctgact cagcagatcc ccctctggct
     7741 tgacgagcac ggcggcaccc tagaggacta cgcgttcgtt cgattggcga cccgataccg
     7801 gtacgttttc ctcgggatcg acaggcggga cggggcgatc gccggcgtgt tgggaacgcc
     7861 cgctccgctg tgagccgact caaaaaaacc agcgctgtgc gatcacgggc tgggtgtttt
     7921 ccacttcctg ctgacgcgcc tcgagctcgc tgacaaactc actatcgccc cgtgcccggg
     7981 ccatgccgag caacgcctcg gcgtaacgag cggccgccgg cttgttttga cgagccagca
     8041 aacgaagcca gggatatatg cgcaggcgca gcagggcctc ggcttcggca tgacgcccct
     8101 catcgccgag cttgtccatg agcgcgtcga tacccggcac gaactgaggg tccatgacca
     8161 gcgtcctcag cagcagctcc tctggcgcct cgccgttttc aagcaggggc aataccgacg
     8221 ggttgcgcat gatgaagtcg gccatcaaga gcagcgtcag cgtgttccac gggtcagccc
     8281 gccccgcccg gcgaaaagca ttcagcgtct cctcgcccag atacctggac tccgggttct
     8341 gtcgcagttt gttgtccagc agcacggctt ccgccagcag gggcaccccc cgatctgcgt
     8401 tgagggaccg ggccataccc gcaaagcgaa gcatgcgctc tggactcttg cgcacctcct
     8461 ccgtgaaggg cacgtagcgc tggttctgca gtatgccggc tgtggcggta tccagaccga
     8521 gatagagcca ggccgtccat ccaaacagaa ccgccaacgt aaccccaaca cgaatcggtc
     8581 gcgcctgagt cgagtcaacg gacccggatc ctttcgaatc acgaaacgac gctgccacag
     8641 taagacccag cacgaagccg agagcgggtg agtagacgac gaagttcacc agggcgtgcg
     8701 cgagcgcggc accaacgccc atggtcagcc cgagcctggc gaaagcctca tctccaggca
     8761 aggcggcaag gcggcgaaaa aacacgatga ccacgacgac cgcgaaggtc accatggcgc
     8821 agaacagtaa cgggccgcct tccagcagca gctgcagata atcgttgtga acatatagtc
     8881 cggccgaatc ctggtctacg accgggcgct ggaaccgata gagcagggga aagacgaaca
     8941 acccgatgcc ggtcgcagga tactcggcat acatggccag cgccgagcca atcaaagcca
     9001 ttcggacctc aatatcggca cctgcaacac ggtccgccgc ggctgcctgg gcagtcaggg
     9061 ccacggactg atccgtcaga gaggctcccg ccagcagata cgccgcggcg gtcaacgcgg
     9121 ccgcagcagc cacaggccgc catctcaatc gtctggctat cgcgagcccg gcccaaataa
     9181 tgaaggcccc cgcgatgacg agcaggcctg cgcgggatcc cgtggcaaaa agcgcgagcg
     9241 ccaggatgaa gcatgccgac agatccttcc cttctaccct caacgacatg ccttgggacc
     9301 cggtcctcag cacaaagtaa cgatgcagcc atggcaggag aatcaggcac aggagggtcg
     9361 catagttgtt ggggtccgtc aggggcagac gtgcacgcac gcctgcgctg aacagcaata
     9421 cgacagagat cgccgcaaag gccagcaccg tcagggatac ggccttgtgc aacgcaaacg
     9481 cagcctcccc tgcgccccgg gcaaggagaa aggcgagcgg cgtgacggca accacccacg
     9541 ccggaacgaa gctggagtcc ttggaaaggc tgaactggta gctgacgacg atgccgacaa
     9601 tggccaaggc cggcagcgcg aaagtcacag gcgaggcgtg gacctctgtt gcaaggcgcg
     9661 gcgccaccaa cgaaagcccg ctcatcagaa cgaagatcgc gccaaagaag acgacgacat
     9721 tgacgtcgct gccgtaaaac acgcagagga gaaggaaaca aacccatccg gcggctacag
     9781 ccgcctggca cgccagatct cctcggggag cgctgtccgg tagcgagatc atagtggttc
     9841 catgagctgt ggtgccggaa cgcggaatcg aaccgcggac ctactgatta cgaatcagtc
     9901 gctctaccaa ctgagctatt ccggcatttg cctggcccca gagggcgcgc agaatagccg
     9961 gttttcagac gggacggaag gtcaactcgg ctcggtgggg agctcagggg cagcggactt
    10021 tttgaggaaa gcgcatgcag accgtcacgc cggtgtcatc cgtcgccgtc acgtcgtagc
    10081 tcccgccgcc gccgtcggcg atggagtagg aaagctcgcc gtcatctctc ggcgtccagc
    10141 cgatgccggc ggtgatcgcc gccgtgttcg ccgcggtcag cagatagctg cccgttcgca
    10201 gcatgcggtc ttcctgaaag atctcgatgg tcgagatgtt gttgaccaga accccctctc
    10261 gcgaggtttg tatgtagccc tggtacaacg gcagcgctac tgcggcaatg atcgcgatga
    10321 tcgctacggc aatcatcagc tcgatcaggg agaatccgtg actagctcgt cctttaatca
    10381 ttgccatggt ccaatgttcc ggatttagac gggttcggcg gacaacagac gagggacccg
    10441 agacgggtcc ctcgtaactt gctcaaccct tagatgctgt tgaacaaaat cgtcgaagcg
    10501 ctctgaccgc tgaacccacc ataattgggc ctggtcacta caatcgtcag atcaccggca
    10561 gcgaaggttc ctgtcttcgc aataccgaca atgccattcg catcgtcagg ggcgacattg
    10621 tagggaaggc cgccgcccgg agccgtgccg ccattgctgt tcagcaaacc ggcccatccc
    10681 gcggcattcg cgtactgggg caatgcgtcg gccgctgcca ggttcagcgt gcggccaatg
    10741 gccacgtctg cctgcacctt gcgcatctcg ttggcaacca gccgggaacc ctcttcgaag
    10801 tgagaggtca ccttcgccat gttcgagttt tcaatgtagt cctgataagc gggcaatgct
    10861 acagcggcga gaataccgat gatggctacc acgatcatca attcgataag ggtgaaaccc
    10921 ttttgcatcg cgtttttcat cttgataaat gctccatatt ttcaaagtca aaacgtggca
    10981 gctcatgcga aaggcacttg cgccgcggtt ttcgcaccct gggaaatgca aacctcgtgc
    11041 cactatgtat cttcactggg ttcacggtcc ctgcagatcc cattttctct tttaagtcat
    11101 agagttatcg cggaacagcg actttgaccc ccgcgccgcc aagtgtcgcc cgtcgtcgcc
    11161 tgtcagtctg cggctacgtc acaaggtgac aaataccggc agttgccgga acgggtcgcg
    11221 agttgccgtt actgccccac gccgggaaag atgatgacgc cggacccgta tcaaatctgt
    11281 gacgccgtta acggctcacc ggtgacgcag gcagcgacgt ttcccgagcg ccacctgtaa
    11341 ggtttcacca acagcgccac caacccggtg gaatgcccgg cgctaggtaa cgaaaaggat
    11401 tgcactgcca aatggcttcc gaccccattc cgctcagcgg cctggccaga cgcctggtcg
    11461 acgaccagat catcgatttt gactccgcca acaaagcaag ccaggaggcg cgcaagcagg
    11521 ggtcgcagtt cgtcaccttt ttagttgaga acgccatggc cgacgctcgc acgatcgccg
    11581 tcagcgcatc ccgtgagttc ggcgtgcccc tgatggacct ctccagcttc gacctggagg
    11641 cgatcccgcg cgatctggtc aaggattcgc tgatcaaaca acacagcgcc ctgcctctgg
    11701 tgcgtcgagg cgcccggctg ttcgtggccc tgtccgaccc caccaatctt caggccctgg
    11761 acgagatcaa attccagacg ggggtcaaca ccgagaccgt gctggtcgag gacgacaagc
    11821 tgcaggtcct gatcgaccag ttcctgaccg cggtcgaaga tgacggtctc ggcgatatgg
    11881 ccaacgacga tctcgacgac ctcgatctcg aggccagtgg cgactcccag gacgacgacg
    11941 aagacgacgt caccagcgga accgactccc gtcccatcgt gcgcttcgtc aacaagatgc
    12001 tgctcgacgc catcaagacg ggcgcttccg acatccactt cgagccctac gagaggagct
    12061 accgcgttcg cttccgcacg gacggcatgc tgaacgaaat atccaagcct ccggtgaacc
    12121 tcggcacccg cctggcggca cgtctcaagg tcatgtcgga actcgacatc tcggaacgtc
    12181 gggtaccaca ggacggccgg atcaagatgc ggctctccaa aacacgcacc atcgatttcc
    12241 gggtcaacac cctgcctacg ctgtggggcg agaagatcgt gctgcggatc ctcgatccct
    12301 ccagcgccaa gatgggcatc gaacatctgg gcttcgagcc cgaccaagag aaaatcctgg
    12361 tcgggaccct gcatcagccc cagggcatga tcctcgtcac gggcccgacg ggatccggta
    12421 agacggtcac cctctacacc gggctgaaca ttctgaacac catggaacgc aacatcgcaa
    12481 cagcagagga tccggtcgag atcaacctcg agggtatcaa ccaggtcaac gtgaactaca
    12541 aggtgggtct cgacttcgcc tcggccctgc gctcgtttct tcgtcaggac cccgacgtcg
    12601 tgatggttgg tgagatccgg gatctggaga ccgccgagat cgcgatcaag gctgcccaga
    12661 ccggtcacat ggttctctcc acgctgcaca cgacgtcggc gccggaaaca ctgacgcggc
    12721 tgcgca
//
LOCUS       DBNHKV010000074         3758 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1641458, whole genome shotgun sequence.
ACCESSION   DBNHKV010000074 DBNHKV010000000
VERSION     DBNHKV010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1641458"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..864
                     /locus_tag="AC16PP_02115"
     CDS             184..864
                     /locus_tag="AC16PP_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6480447.1"
                     /translation="MAEEQELIVEQIEIGPMQNFTYIVGSRSAREVAIVDPAWDVEHL
                     VRHIEEKGYKLTAALVTHYHPDHIGGSFGGHNIDGIAELLAINPLKVYAHKEEAEGVR
                     KVTGISESDLVRVESGDDLRIGDVSIEFLHTPGHTPGSQCFRVHNTLVSGDTLFIQGC
                     GRVDLPGSNSEDMYHSLQKLKSLPDETLLLPGHNYAHVPNATMGETKKFNTYMHVKDL
                     QTWRMIMG"
     gene            1002..1253
                     /locus_tag="AC16PP_02120"
     CDS             1002..1253
                     /locus_tag="AC16PP_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480448.1"
                     /translation="MNAYDHLVGQHFLRAGVRYTVVKCVGSTAITAYLRDNRIYRVVM
                     RVSDVLDLLEVTEISITVPAMEPKKALPAPQLERASGQS"
     gene            1409..1759
                     /locus_tag="AC16PP_02125"
     CDS             1409..1759
                     /locus_tag="AC16PP_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480449.1"
                     /translation="MREQPVFETQGLWVRWSWDEKRKSLKLEKAEPRPQPATAQPTSH
                     WIFTLRDQLVSRSSRLLLRVGVWVLRQRMSSAEVGEYLRETADRVASGHHEPGDPLES
                     HARRCNWNHFFVAK"
     gene            complement(1804..2778)
                     /locus_tag="AC16PP_02130"
     CDS             complement(1804..2778)
                     /locus_tag="AC16PP_02130"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR6480450.1"
                     /translation="MKDPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQLLEIT
                     PALNALQGVVMELEDCAFALLHGVVATDDPNVAFKDATHALLVGSRPRSKGMERKDLI
                     EANAAIFSVQGKALNDHADRAVKVLVVGNPANTNCLIAQRNAPDLDPRQFTAMTRLDH
                     NRAAAQLAARTGKRVTDVAGLCIWGNHSATQYPDLHAATVAGTPAMELVDMAWYTGEF
                     IPTVQQRGAAIIEARGASSAASAANAAIDHMHDWSLGSEGVVSMGVYADGSYGVEPGL
                     IYSFPVICEGGDWQIVQGQEIGEFSREKMAATEKELVEERDAVAHLLS"
     gene            complement(2893..3324)
                     /locus_tag="AC16PP_02135"
     CDS             complement(2893..3324)
                     /locus_tag="AC16PP_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR6480451.1"
                     /translation="MERDRFPVVVHVLMFRGDELFLLRRASTGFMDGYYALPGGHQRA
                     GESVAQAARRECEEETGVTDLDLAPICVMPYRAGRHQGLNFVFEAASWLGEPRVAEPE
                     LFSEGVWAAARDLPEPHAMWISDALQCRDEGDWFREFLWHG"
     gene            3393..>3758
                     /locus_tag="AC16PP_02140"
     CDS             3393..>3758
                     /locus_tag="AC16PP_02140"
                     /inference="COORDINATES: protein motif:HMM:NF049302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase, cg3035/Rv0428c
                     family"
                     /protein_id="HIR6480452.1"
                     /translation="MTRAGEPDAALTRRIEEASLNAWPAMEQLLMDGWLLRFSRGFTK
                     RANSVVPLYPWTGNGREPLAERVRHCENLYAREGLQTIFRLPAIDDNTALDAHLESRG
                     YNLVDPTLVLVAPTRPGPGH"
BASE COUNT          721 a         1290 c         1099 g          648 t
ORIGIN      
        1 tcgtgccgtt caatctgctc gtcaaacgag tattcccacg catccggggc gtttaccggg
       61 tggatggttt gaccctttac gtctctccgg gcaccggaac gtggggcccc attctccgct
      121 taggatctcg ttcagagatc agtatgatga cccttggctg aagaaacgac cggacaagca
      181 atcatggcag aagagcagga actcatcgtc gagcagatcg agatcgggcc catgcagaac
      241 ttcacgtaca tcgtggggtc tcgatcggcg cgcgaggtgg ccatcgtgga cccggcatgg
      301 gacgtggagc acctcgtgcg ccacatcgag gaaaagggct acaagctcac cgcagcgctg
      361 gtgactcact accatcccga tcacatcggc gggtcctttg gcgggcacaa catcgacggc
      421 atcgccgagc tgctcgccat caatcctctc aaggtctacg cgcacaagga ggaagccgaa
      481 ggagttcgca aggtcacggg catatccgag tccgatctcg ttcgcgtgga gtccggcgac
      541 gatctgcgca tcggagacgt gagcatcgag ttcctgcaca cgcccgggca cacgcccggc
      601 tcccagtgct ttcgagttca caacacgctg gtgtccggcg acaccctctt cattcagggc
      661 tgcgggcgcg tggaccttcc cggatcgaac tccgaggaca tgtaccacag ccttcagaaa
      721 ctcaaatcgc tccccgacga gacccttctt ctgccgggac acaactacgc ccacgtcccc
      781 aacgcaacca tgggcgagac gaaaaaattc aacacctaca tgcacgtcaa ggatctgcag
      841 acctggcgca tgatcatggg ctgacgcgcc tgccgccgat cggccggttt ccgccattcg
      901 tcatcgccgc tcgccgggcg gccgctaaac cccgactgcc ggtcgtgcgt cccggcgatc
      961 cgcgccgcta agattccgta ggagttgaag aggaattcga cgtgaacgcc tacgatcatc
     1021 ttgttggcca gcatttcctg cgcgccggag tgcgctacac cgtggtcaag tgtgtcggct
     1081 cgaccgccat caccgcctat cttcgcgaca accggatcta ccgggtcgtg atgcgcgtga
     1141 gcgacgttct cgatctcctg gaagtgacgg agatcagcat caccgttccc gcgatggagc
     1201 ccaagaaagc cctgccggca ccgcagctcg agcgcgcatc cggccagtcc tgacaaggcg
     1261 ccgccctggg tatcccaggc cggacctttc ctcccggttc cgccaagggg gcatgagcct
     1321 gccaacgcgg ctagtctcaa aggccggtta acgaaccgct tacatcactg cgatatcagt
     1381 tgagctcgga cacctataga ttaagccgat gagagagcag ccggtatttg agacgcaggg
     1441 cctgtgggtc cggtggagct gggacgagaa gcgcaagtcc ctgaagctcg agaaggcgga
     1501 gccacgtcct caaccggcca ccgctcaacc tacgtcgcac tggatcttca cgctccgcga
     1561 ccagctcgta tcgcgcagca gccggctgct gcttcgcgtc ggggtgtggg tgctcagaca
     1621 acggatgtcc agcgccgagg tcggcgagta cctgcgcgaa acggccgacc gcgtcgcaag
     1681 cggtcaccac gagcccggcg accccctgga aagccacgcc cggcgctgca actggaacca
     1741 cttcttcgtc gccaagtagc ggtttgcgaa agctgctgcc tccgccagcc acgacaggcc
     1801 gtttcagctc agcaggtgcg ctacggcgtc gcgttcctcg acgagttctt tctctgtcgc
     1861 agccatcttc tcacggctga attctccgat ctcctggccc tgcacgatct gccagtcgcc
     1921 accctcacag atgaccggga aggagtagat cagccccggc tcgacgccgt agctgccatc
     1981 cgcgtagacg cccatgctca ccacgccctc gctgcccaga gaccagtcat gcatgtggtc
     2041 gatggcggcg ttcgctgccg aagccgcgct ggacgctccg cgggcctcta tgatcgcggc
     2101 cccccgctgc tgcaccgtgg gaatgaactc ccccgtgtac cacgccatgt cgacgagctc
     2161 catggcgggc gtgccggcaa cggtcgccgc gtgaagatcg ggatactgcg tggccgaatg
     2221 gttgccccag atgcacagcc cggcaacgtc cgtcacccgc tttccggtcc ttgccgccag
     2281 ctgcgcggcc gcacggttgt gatccagccg ggtcatggcg gtgaactgcc ggggatccag
     2341 gtcgggcgcg ttgcgctgag cgatgagaca gttcgtgttc gccgggttgc caaccacgag
     2401 caccttcacc gcgcgatccg catggtcgtt gagcgccttg ccctgcacgg agaatatcgc
     2461 cgcgttcgct tcgatgaggt ccttgcgttc catccccttg cttcgcgggc gtgagccgac
     2521 cagcagcgca tgggttgcgt ccttgaaagc cacgttggga tcgtccgtgg cgacgacccc
     2581 atgaagcagc gcaaacgcac agtcttccag ctccatcacc accccctgaa gcgcgttcag
     2641 cgcaggcgtg atctccagga gctgcaggat caccggctga tcctttccca gcatctcccc
     2701 ggaagcaatt cgaaacagca atgcatatcc aatctgaccg gcagcgccgg taaccgcgac
     2761 ccgcacggga tctttcatgt ctgactccct ttcacaatct cgactggccg cgcgtgccga
     2821 tacggcatgt cgttacgccg gcccatagct cagcggcggc gcattgtaag gaaagcaaat
     2881 cgctttcgca cgtcacccgt gccagagaaa ctccctgaac caatccccct cgtcccgaca
     2941 ttgcagcgcg tcggaaatcc acatggcgtg gggctcggga agatcacgcg cggccgccca
     3001 tacgccttcc gagaacagct ccggctcggc aacgcgcggc tcgcccagcc aagacgccgc
     3061 ctcgaagacg aagttgaggc cctgatgccg accggctcga tacggcatca cgcagatcgg
     3121 cgcgaggtcc aagtcggtta cacccgtctc ttcctcgcac tcgcgccgag cggcttgcgc
     3181 aacactctca cccgcgcgct gatggccgcc cggaagggcg tagtagccgt ccatgaaccc
     3241 ggtgctggcg cgccgcagga gaaagagctc gtcgcccctg aacatcagca cgtgcaccac
     3301 caccggaaac cgatcgcgtt ccaccagacc cgctcaaacc ccttttgatc ctgctatggt
     3361 gcgcgggtga atgatgccga gcaaccccgc ccatgacccg agcaggcgag ccagacgcgg
     3421 cgttgacccg acggatcgag gaggcgtcac tgaacgcgtg gccggccatg gagcaactgc
     3481 tcatggacgg ctggctgctc cgcttctccc gcggcttcac caaaagggcg aacagcgtcg
     3541 ttcccctcta cccctggacc gggaacgggc gcgagccact ggcagaaagg gtgcgccact
     3601 gcgaaaacct gtacgcccgg gaaggcctgc agaccatatt ccggctgccg gccatagacg
     3661 acaacaccgc tttggatgcg catctcgaaa gccgcggcta caacctcgtc gacccgaccc
     3721 tggtgctcgt cgcaccaacg cggccgggcc cgggccac
//
LOCUS       DBNHKV010000075        20197 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1286678, whole genome shotgun sequence.
ACCESSION   DBNHKV010000075 DBNHKV010000000
VERSION     DBNHKV010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 20197)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20197)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20197
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1286678"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..685)
                     /locus_tag="AC16PP_02145"
     CDS             complement(161..685)
                     /locus_tag="AC16PP_02145"
                     /inference="COORDINATES: protein motif:HMM:NF016121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR6480453.1"
                     /translation="MIIGCVLTLFGPALLAGAAVYQKPDEFLAEAFVGDAPSAQALWI
                     KAELKHELSQVLAHPPGLRVRYWQAGSRTAWILDEIGKDLPITAGIVINDGAIEDVRV
                     LEFRESRGWEIRYPFFTRQFQNARLQGDRLTQNIDGITGATLSVRAMKRMATAALLLH
                     EHSSASSTTLAKAP"
     gene            complement(757..2010)
                     /locus_tag="AC16PP_02150"
     CDS             complement(757..2010)
                     /locus_tag="AC16PP_02150"
                     /inference="COORDINATES: protein motif:HMM:NF019042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR6480454.1"
                     /translation="MRVVVVMAAMALVSSATAEEVTLEQLWAVVQAQQAQIEALTDAL
                     ETTQAQLASTEEQVEVAEEQIGLTADYLAEIEASSTRGSRTQLGGYGELHYNNVDARD
                     PDESFKEMDFHRFVLFFSHEFTDRIRFHSELELEHSLVKDTADGSNGGEVELEQAYVE
                     MDLNENHWTRAGLFLLPVGILNETHEPNTFYGVERNDVESIIIPSTWWEGGVAAGGRY
                     GNGLSWEAGVHSGLAMPTDGGNAFRVRSGRQKVSNASAENLAYTLRLKYTGIPGLELA
                     GSYHHQSNPSQMDEDGLDGGDLFSFHGIWSQGPFTLKGLWARWNFDGDLVESSGADRQ
                     TGWYLEPSVRLDLAGTDWGFYGRYEDLEGGRTQDRFDQWEVGFNFWPTENVVLKFDYR
                     DRDHDLETELGRDFKAIDLGVGYQF"
     gene            complement(2127..4220)
                     /gene="feoB"
                     /locus_tag="AC16PP_02155"
     CDS             complement(2127..4220)
                     /gene="feoB"
                     /locus_tag="AC16PP_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR6480455.1"
                     /translation="MTTVALVGNPNCGKTTLFNALTGTRQRVGNWPGVTVERKTGSYS
                     HGDGSVDVVDLPGTYSVHPVGDAVDERIAREFISAAEPSGPDVVVNVVDASSLARGLY
                     LTTELLQMGKPVVVALNMIDVADRNGMHIDTFQLARELGCPVVALVASRGDGVGALKD
                     AVESREGAGPASALPPMSEPDERFHYVDGLITRCVKATPTRRSVTDRIDAVVLHRYLA
                     YPIFLGVMYLMFMFAINVGSAFIDLFDGVGNVLFVEGPRRAMLAVGLPEWLVVFAADG
                     VGGGIQLVGTFIPVIGCLFLALSFLEDSGYMGRVAFIVDRLMRRLGLPGKSFVPLIVG
                     FGCNVPAVMATRTLDNEPDRILTTIMAPYMSCGARLTVYALFAAAFFPTGGQNVVFAL
                     YLIGIVVAVLSGLAVRRYLISDSRSTFLLELPTYHLPTMKGLLLQTWQRLKGFVLRAG
                     KAIVAVVIVLNVVGSVGTDGSFGNQNTEKSVLSALGRTITPIFHPMGISQENWPATVG
                     IFTGIFAKEVVVGTLDALYAPGEVASADDLAGQLRAAAASVPANLAELGEQLTDPLGI
                     GLGALDDVAEAASDQEVEINTIQAMRELFHGKLGAFSYLLFILLYMPCVATIGAIFKE
                     IGGFWAAFSTSWSVTVAYSAAVICYQLGNLAEQPGPAMLWIAGVAAATVAAFAGLVAM
                     GRRRRQRLIPLVNLE"
     gene            complement(4217..4441)
                     /locus_tag="AC16PP_02160"
     CDS             complement(4217..4441)
                     /locus_tag="AC16PP_02160"
                     /inference="COORDINATES: protein motif:HMM:NF015956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR6480456.1"
                     /translation="MSLRDLSPGDMARVSGYREDDDYAAQLMRLGLIPGTEIRVQRRA
                     PLGDPMEIRFRGFSLALRPSEADALMLELI"
     gene            complement(4550..4960)
                     /gene="fur"
                     /locus_tag="AC16PP_02165"
     CDS             complement(4550..4960)
                     /gene="fur"
                     /locus_tag="AC16PP_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HIR6480457.1"
                     /translation="MPGSDIKRAGLKVTLPRLKVLEVLESAEPHHLSAEDVYKSLIDS
                     DDSVGLATVYRVLTQFETAGIVSRHNFDDGHSVYELAGDEHHDHMVDMDTGQVIEFVN
                     ERIEALQREIAQEHGFELIDHELVLYVRKQGKSE"
     gene            5092..5898
                     /locus_tag="AC16PP_02170"
     CDS             5092..5898
                     /locus_tag="AC16PP_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6480458.1"
                     /translation="MADLKGKVAVVTGASRGVGRGVAEGLAEAGATVYVTGRSESHDN
                     PPQERTIQATAASVDALGGKGIAVRVDHNEDAEVKALFERVMSEQGRLDVLVNNVYKI
                     PDPPVFDGGFWEHPVSVWDDQCGVGLRGYYVASVFAAPMMVARRSGLIVNISSRGGDQ
                     YVFSTSYGVGKAGVDRMARDMAHELRGYHVAAVSLRPSSVKTEFILDAVASGAVKLDM
                     AKAQSPRFTGRCVAALAMDPDLMEKSGGVYMVADLAAEYRFTDPDRVEAS"
     gene            5900..6622
                     /locus_tag="AC16PP_02175"
     CDS             5900..6622
                     /locus_tag="AC16PP_02175"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6480459.1"
                     /translation="MQPVERHVDVATPDGSMNTFIAYPQGGGPFPVVVFYMDAPGKRE
                     ELHDMARRLASAGYYVMLPNLYYRRVREFVMTAEARPEMMAHMNSLSNHMACEDTAAL
                     FAFASADRAARDGPAGCVGYCMSGPFAFAAAAAFPDRIAAAASIHGVRLCTDAIDSPH
                     LNAEHVGGELYFACAEHDDWAPPEMIEALEGHLSRVGASYRIEWYPGTSHGFVFPARP
                     VYHPAAAERHWERVLDLFSRNL"
     gene            complement(6606..7676)
                     /locus_tag="AC16PP_02180"
     CDS             complement(6606..7676)
                     /locus_tag="AC16PP_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HIR6480460.1"
                     /translation="MLIDRDALTRFVSEIFVRLDAEASDAQEVAAHLVSANLKGHDSH
                     GVGMVPTYVGNIRSGALALNTHARLVRDKGAVMLIDGQMGFGQVVGREAVELAIERVR
                     QTGVVAAGVRNCHHLGRIGHYGEICGRAGYVSMHFVNVVGHQPLVSPFGGRQARISTN
                     PFCCVVPRSDEPPVVLDMATSAIAQGKVRVAYMKGDKVPDGSLIDHEGKPTNDPSVMF
                     EDPRGSLGPFGHHKGYGLALMCELLGGGLAGEWTAQAPPPDRFTIVNHMLMVVLDPDL
                     FGGAEAFHREVEEMIAYLRSTMPAAGVDRVRVPGEPELESMAERAAGGIPIDDNTWAA
                     IRKSAQVSGMTEEEIAGFTGSG"
     gene            7757..8614
                     /locus_tag="AC16PP_02185"
     CDS             7757..8614
                     /locus_tag="AC16PP_02185"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR6480461.1"
                     /translation="MDKCAEGFSLIEGPVWDPDRGLLFSDVLVGGVFCLGGDGTVTTV
                     FEHRRGIGGMALHEAGGLIVSGRNLSFKPYDEGSTVTVLDRDEENGLVGFNDLTTDAE
                     GRIYAGSLGASPVFADGREPRAGDLYLVDLDGSARVVAEDVRLTNGLGFSPDGTTLYH
                     SESGRRTVFRYDVQPDGSLSAKEPFVTPDNGVPDGLAVSRDGAVWVALARGGHGVAVY
                     EASGRLREFIEIPLPMCTSVCFGGEDLKDLYIVSGSAGAGSDRAGAVFRERVDVAGLI
                     MTPARVRLA"
     gene            8682..9512
                     /locus_tag="AC16PP_02190"
     CDS             8682..9512
                     /locus_tag="AC16PP_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6480462.1"
                     /translation="MAIEPMDTGTEDLLAHQEDGVAVITLNRPQARNAMSAAMNAGLA
                     KALDEAERNPAVRCVVITGAGGAFCAGGDVKGMAARSQGGGPGPTVDERIHLQRLNQR
                     ATAGRIYQMPKPVLASLPGPAAGAGLALALAADLRIMADSAFITTAFAKVGFSGDFGG
                     SFFLSRLVGSGKAKELYFLSDRVSAAECERLGIANAVVPAEELEAQTMALARRLASGP
                     PVAYRYMKENFNRAAAGAGVLECLDLEATHHVHTGLTEDHRAAAKAFVEKTEVEFKGR
                     "
     gene            complement(9550..10527)
                     /locus_tag="AC16PP_02195"
     CDS             complement(9550..10527)
                     /locus_tag="AC16PP_02195"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR6480463.1"
                     /translation="MDIKDGFAGTIGNTPLIRMNRLSEETGCEILGKAEFLNPGGSVK
                     DRAALWMVREHERSGALKPGGVVVEGTAGNTGIGLVHVCNARGYRSIIYMPDTQSPEK
                     VQLLKTLGAEVREVPAVPYRDENNYQKQAGRCAETLENGVWANQFDNVANRLAHYEST
                     GPEIWDQTEGRVDAFTCATGTGGTLAGVSRFLKERREDVRIVLADPMGSALYNWVTTG
                     ETTMSQGPSITEGIGNSRVTDNLAGTCIDDAVQVTDRDAVNMVYRELREEGWFFGSST
                     GINLCAAVQVAHSMGPGHTIVTVLCDTGAKYQSRLFNPEWLAERGLTPG"
     gene            10618..11784
                     /locus_tag="AC16PP_02200"
     CDS             10618..11784
                     /locus_tag="AC16PP_02200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6480464.1"
                     /translation="MKKADLQDRWLGDAGENGVNPLRIAALMARVRKEVDEGLLPAAQ
                     VALARNGALVLFETMGAADNDSLFCVFSATKAITSAAAWILMQEGRLKEDELVADIIP
                     EFAANHKHDITVGQLFVHTAGFPSAPFKPLDWNDRARRLERFAQWQLNWPPGSRYEYH
                     PTSSMWVIAEIIERRANTTYQEFVRERIALPLGLPELYVGLPATENERVVPNVHVGDA
                     LTRADYELLGLPEPPVTEVTEEAILKFNRPEVRAVGVPGGGGIMSAADLALFYQGLLH
                     GGLGEEVWSAATLADARRVRSGELRDPVMGHRANRALGLVIAGDPQRNLRGFGKTNSP
                     ETFGHNGAGGQLAWADPATGLSLAYCTAGHDRNRIRQGRRGVAISSLAAVCCED"
     gene            complement(12307..12702)
                     /locus_tag="AC16PP_02205"
     CDS             complement(12307..12702)
                     /locus_tag="AC16PP_02205"
                     /inference="COORDINATES: protein motif:HMM:NF039797.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HIR6480465.1"
                     /translation="MPSTSKFAKLACSIAGTVLLYASSGLALPPDSPIGPRAESIVEG
                     TVQAPVSSIPAANRIEGWARVDDKRVVLEVSPQTRYLLTLRQGCIGLGFARNVSVTMS
                     NNTIYAGFDAITADGEHCEIRTINPLVTP"
     gene            12990..14360
                     /locus_tag="AC16PP_02210"
     CDS             12990..14360
                     /locus_tag="AC16PP_02210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6480466.1"
                     /translation="MAASAESVLERVDAEAAAAVSMAERLWALAELGYQETRSSELLG
                     AYLAESGFSLESGVAGIPTAFVATFGSGRPVIGVLAEFDALPGMSQAAEPSRRPLEPG
                     GAGHACGHHLFGAGAAAAAVSVARWMEANGVPGSIRVYGTPAEEGGSGKVYLTRAGLF
                     DDVDVVLHWHPGSRNDASPATTTANKSGRFTFRGVAAHAAAAPERGRSALDGVESMNY
                     MVNLMREHVPSDSRIHYVITKGGDAPNIVPEIAQVYYYVRHPEAATVIELFERVVAAA
                     RGAAMGTETDVDYEVMHGNYAVLPNETLAKRVHAHLLSLGGVSYSPDERRFAEQIQAT
                     LGDSLPPLSRAASVEPFQMRQNMGSTDVGDVSWNVPTVGFYAATWVPGTPAHSWQAVA
                     AGGTSIGHKGMLLATRTLALTAADLLRTPSLIAAARAELEERRGQDFRYVALLGERDP
                     PLDYRR"
     gene            14357..15199
                     /locus_tag="AC16PP_02215"
     CDS             14357..15199
                     /locus_tag="AC16PP_02215"
                     /inference="COORDINATES: protein motif:HMM:NF020694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6480467.1"
                     /translation="MNLGVLHPGEMGVSVGAVLTAAGHRVYWLPESRSRATRERAEGA
                     GFTGCASLRELADRVAGIVSVCPPAAAEEVASQVRSAGFTGVYVDANAVSPATARRMA
                     RTMGPTYVDGGIVGPPAVRPGLARLYLSGAAAEEVAGWFHGGLLEARVLAGEAGAASA
                     LKMCYAAYTKGSSALVLAIRALAEAEGVDEPLLAEWDISQPGLADRSQRAAAGTAAKA
                     WRFVGEMEEIAATFRGVGLPGEFHEAAAVVYRRLAPLKGNAAAELADVVRRLIENPAD
                     VGED"
     gene            15183..16223
                     /locus_tag="AC16PP_02220"
     CDS             15183..16223
                     /locus_tag="AC16PP_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480468.1"
                     /translation="MSGKISRIARMAVSWAIAAPAIAAPGYLFDEAATLDLELEAPLG
                     ALMGDRYPDSEYRPAQLRYVGKDGSRVALPVEVRTRGKTRRDEEVCKMPPLRVRFDQA
                     AADGTPFAGQPALKLVTYCRDSDSFDQLVLLEYLAYRTYNLLTPQSHRVRLARISYLE
                     RGDRRTTRYGIFLEDWRAVAARNDLTADPVDGGVNVDKLNHDAANRVAVFAYLIGNED
                     YSLLWPEPDDNCCHNIKPLLDRDGRVVPLPYDFDYSGLVNAPYAVAKNPRRKVRQRAY
                     RGLCDTQEGLEAALELTRARREDIYAVFRAQEGLSPAKLKSSMGYLDRFYDLIDQPAQ
                     VEKRLRRRCRKS"
     gene            complement(16228..16608)
                     /locus_tag="AC16PP_02225"
     CDS             complement(16228..16608)
                     /locus_tag="AC16PP_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480469.1"
                     /translation="MGYPATKLTVILIVSAIAAPAFANPVQCTQGSLVRSVEVVYSGP
                     DQTLPCEVIYDKTQEGAGIHSLWRASNEAGYCEAQAAAFADKLGAMGWDCGEPAGSGI
                     DVDSGDAPSAADVPAEIPIAPDPA"
     gene            complement(16625..18211)
                     /locus_tag="AC16PP_02230"
     CDS             complement(16625..18211)
                     /locus_tag="AC16PP_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR6480470.1"
                     /translation="MALRPPQPDADHMSWEEEIRELRKREQLAYQMGGAEKVARQHEF
                     NKLTIRERIAAISDSGSFQEIGTLAGVGQYDDDGNLIGFTPSNFVFGAAEIDGRPVIL
                     SGDDFTVRGGSADASIIGKRLQAEGLATELRLPHVRLVDGMGGGGSVKTIEMAGRTYI
                     PEVRGWETVVQHLAVAPSVSLALGSVAGIGAARVATSHYSVIVKNSAQMMIAGPALVD
                     WASLGDVSKEELGASRIHTRNGAIDDEASSEEEAFDMARRFLSYLPTSTDELPPVTDA
                     TDDPERTDEALLEIVPKDPRKVYKMHRIIDSVVDEGSFFEIGRRWGRSIITGLARLNG
                     MPVALFAENPMVYGGAWTADASRKLVRFIDLASTFHLPLVHLEDCPGFLIGKQSEESG
                     TIRFGCQALAALGQAPVPFCCVVVRKAFGVAGASNHKPGSHCVRMAWPSGDWGSLPIE
                     GGIEVAYKADLAQADDYDAHLSRIKERLNRLRSPFRSAEHFEIEQIIDPRETRPALCR
                     WMKLARQALRTDPPTFWFRP"
     gene            complement(18240..18605)
                     /locus_tag="AC16PP_02235"
     CDS             complement(18240..18605)
                     /locus_tag="AC16PP_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480471.1"
                     /translation="MFVYQARYAILRALNCNDWRDGHMSADGTWNTVMNTPMGAQNGT
                     MTLATDGGTLTGTLKGPQGEIELKDGTADGDSLAWKADITTPMAMTLEFSATVNGDEM
                     TGNVKLGAFGDATFTGTRA"
     gene            18632..19327
                     /locus_tag="AC16PP_02240"
     CDS             18632..19327
                     /locus_tag="AC16PP_02240"
                     /inference="COORDINATES: protein motif:HMM:NF053177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8091 domain-containing protein"
                     /protein_id="HIR6480472.1"
                     /translation="MSIGTLNEGPLHQAIKSLYNGAGAREEVPVGDFVADVFGPDEVI
                     YEIQTGGFGSLKKKLARLVEDHRVVLVHPIAEVRYIVKLPQGEDQQASRRRSPKRGSV
                     AHVLDRLVSIPALLNHPNFELEVLLIEEEELRTFDPRRVRRRDGWRVVQRRLGSVLAS
                     ERFTCAGDLLRMVPGPLPAEFTTEDLAGAMGQPRWLAQKLAYCLREAGEISISGKRGN
                     ALCYRLARSGAVA"
     gene            19383..20060
                     /locus_tag="AC16PP_02245"
     CDS             19383..20060
                     /locus_tag="AC16PP_02245"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6480473.1"
                     /translation="MIELYTASTPNGWKASVTLEELGLPYEVRPVDLMAGEQKAPEFL
                     KINPNGRVPAIVDDGFPIFESGAIMIHLAEKTGRLMPADAHGRSLVIQWLMFQMGGVG
                     PMMGQANVFYRAMEEKIQPAIDRYQNECRRLFEVLDGRLRNSEWLADHFSIADIANWC
                     WARLYYWSGVSLDGLDAMQRWMAAMEARPACQRGVEVPNRLELPRDDDPDGIERQALQ
                     SRRVLVR"
BASE COUNT         3364 a         6490 c         6793 g         3550 t
ORIGIN      
        1 cgcctgtagc ccgtaccaat cgagcaccca ctcgctggca acgtgacgtt gcccgaggtc
       61 cagctctgcg gcgtattgaa gcggcacccc ggtagcgagc aggtagacga gcacgagcac
      121 cgcgcagatg cccgcggttc tgtgccattg attgacccgt ctagggtgcc ttggcgagcg
      181 tggtgctgct ggcgctggaa tgctcatgaa gcagaagcgc ggccgtggcc atacgcttca
      241 tggcccggac ggagagggtg gcgccggtaa taccgtcgat gttctgggtc aggcgatcgc
      301 cctggagccg ggcattctgg aactgccgcg tgaagaacgg atagcggatt tcccagcccc
      361 ggctttcgcg aaactcgagg acccgtacgt cctcgatggc gccgtcgttg atcacgatcc
      421 cggccgtgat gggcaggtcc ttgccgatct catccaggat ccaggcagtc cgggatcccg
      481 cctgccagta gcgtacgcgc agcccgggcg ggtgagcgag cacctgagaa agctcgtgct
      541 tgagctctgc tttgatccac aacgcctggg ccgacggggc atcgccgacg aaagcctcgg
      601 cgagaaactc gtcaggtttc tgatacacgg ccgcgccggc cagcagggcc ggcccgaaaa
      661 gggtgagaac acaaccgata atcattctta ttttcatatg ggtcgatttt gccgtgatcg
      721 gtgcaaaaat caattccggg cttgtaagac ggctgctcag aactggtagc cgacgcccag
      781 gtcgatggcc ttgaagtcgc gcccgagctc cgtctccagg tcatggtcgc gatctcggta
      841 gtcgaatttc agcaccacgt tctccgtggg ccagaaattg aagccgactt cccactgatc
      901 gaatctgtcc tgggtccgac cgccttcgag atcttcgtag cggccgtaga atccccagtc
      961 ggtgccggcc agatcgagcc ggacgctggg ctccaggtac cagcccgtct gccgatcggc
     1021 gcccgaggac tcgacgagat cgccgtcgaa gttccagcgc gcccataggc ctttcagagt
     1081 gaaaggcccc tggctccaga tgccgtgaaa gctgaacagg tcgccgccgt cgagaccgtc
     1141 ttcgtccatc tggctggggt tgctctgatg gtgatagctg ccggccagct cgagcccggg
     1201 aatcccggtg tatttcaggc gcagggtgta ggcgagattt tcggcgctgg cgttggacac
     1261 tttctggcgg ccgctgcgca cgcgaaacgc attgccgccg tcggtgggca tggccaggcc
     1321 gctgtgcacg ccggcctccc aggacaggcc gttgccgtag cgccccccgg ccgccacgcc
     1381 cccctcccac caggtggacg ggatgatgat gctctccacg tcgttgcgct cgacgccgta
     1441 gaaggtgttc ggctcgtggg tctcgttgag aatgccgacg ggcagcagaa aaagaccagc
     1501 cctggtccag tggttctcgt tgaggtccat ttccacgtag gcctgctcca gctccacctc
     1561 gccgccgttg ctgccgtctg cggtgtcctt gaccagtgaa tgctccagct ccagctccga
     1621 gtggaaccgg atccggtcgg tgaactcgtg actgaagaac agcacgaagc gatggaagtc
     1681 catctccttg aaactctcgt ccggatcgcg ggcgtcgacg ttgttgtagt gaagctcgcc
     1741 gtaaccgccc agctgggtgc ggctgccgcg cgtgctggac gcctcgatct ccgcgaggta
     1801 gtcggcggtg aggccgatct gctcctcggc gacctccacc tgctcctcgg tgctggcgag
     1861 ctgggcctgg gtggtttcga gcgcgtcggt cagcgcttcg atctgggcct gttgagcctg
     1921 cacaacggcc cacagctgct ccagggtgac ttcctcggcg gtggcggacg aaaccagggc
     1981 catggcggcc ataaccacga cgacacgcat gagcgattct cccgaacgtg ttggatcaag
     2041 gggaacggga gtctagcgga tgtgtttatg aataggaatt attattattt agattaaggt
     2101 attggtcttg ccgcagcgcc gtgggcctat tccaggttga ccagcggaat caggcgttgc
     2161 cgacgtctgc ggcccatggc gaccagcccc gcgaaggcgg cgacggtggc cgccgcgacg
     2221 cctgcgatcc acagcatggc cgggcccggc tgctcagcga ggttgcccag ctggtagcag
     2281 atgaccgcgg ccgagtaagc gacggtcaca gaccagctcg tgctgaacgc cgcccagaag
     2341 ccgccgatct ccttgaagat ggcgccgatg gtggccacgc agggcatgta cagcaggatg
     2401 aacagcaggt agctgaacgc gccgagcttg ccgtgaaaga gttcgcgcat ggcctgaatg
     2461 gtgttgatct cgacctcctg gtcggaggcg gcctcagcca cgtcgtccag ggcccctagc
     2521 ccgatgccca gcgggtcggt gagctgctcg ccgagctcgg caagattggc ggggaccgaa
     2581 gcggcggctg cccggagctg gccggcgagg tcgtccgcgg acgccacctc gcccggtgcg
     2641 tacagggcat ccagagtccc gaccacgacc tccttggcga agatgccggt gaagatgcca
     2701 acggtagccg gccagttttc ctggctgatg cccatgggat gaaagatcgg ggtgatggta
     2761 cgccccagag cggagagcac agacttctcg gtgttctgat tcccgaacga gccgtcggtg
     2821 cctaccgatc ccaccacgtt gagaacgatc accacggcca cgatggcctt gccggcgcgc
     2881 aggacgaagc ctttgagccg ttgccaggtc tgcagcagca gccccttcat cgtcggcagg
     2941 tggtacgttg gcagctccag caggaacgtg cttcggctgt cggagatcag gtaccgtcga
     3001 accgccaatc ccgacagcac cgcgacgacg atgccgatca ggtagagggc gaataccacg
     3061 ttctgcccgc cggtggggaa gaacgccgcg gcgaaaagcg cgtagaccgt cagccgggcc
     3121 ccgcaggaca tgtagggagc catgatcgtg gtgaggatgc ggtccggctc gttgtccagc
     3181 gtgcgagtcg ccatcaccgc cggcacgttg cacccgaagc ccacgatcag cggcacgaac
     3241 gacttgcccg gaaggcccag gcgtcgcatg agccggtcga cgatgaacgc gacgcgcccc
     3301 atgtagccgc tgtcctcgag aaacgacagc gcgagaaaca ggcacccgat caccggaatg
     3361 aaggtcccga ccagctgtat gccgccgccg acaccgtctg ccgcgaacac gacgagccat
     3421 tccggcaggc ccacggccag catcgcgcga cggggaccct cgacgaacag cacgttgccg
     3481 accccgtcga acaagtcgat gaacgcagag cccacgttga tggcgaacat gaacatgaga
     3541 tacatgacgc ccagaaagat cgggtaggcc aggtagcgat ggagcaccac ggcgtcgatc
     3601 cggtcggtca cggagcggcg ggtcggtgtg gctttgacgc agcgggtaat cagcccgtcg
     3661 acgtagtgga acctctcgtc gggctcgctc atgggcggca gggccgaggc ggggccggcg
     3721 ccttccctgc tctcgacggc gtccttgagg gcccccacgc cgtccccccg gcttgccacc
     3781 agcgcgacga ccgggcagcc gagctcccgg gcgagctgga aggtgtcgat gtgcatcccg
     3841 ttgcggtcgg cgacgtcgat catgttcagc gcgacgacca ccggcttgcc catctgcagc
     3901 agctcggtgg tcaggtagag cccgcgggcg agactcgatg cgtccaccac gttgacgacg
     3961 acatccgggc ccgatggctc agcggcgctg atgaactccc gggcgatgcg ctcgtccacc
     4021 gcgtctccga cgggatgcac gctgtaggtg ccgggcaggt cgaccacgtc cacggaaccg
     4081 tcgccgtggg agtagctgcc cgttttgcgc tccaccgtaa cgcccggcca gttgcctacg
     4141 cgctgacgcg tgcccgtcaa cgcgttgaac agcgtcgtct tgccgcagtt gggattgccc
     4201 accagggcga cggtggtcat atcagttcca gcatcagcgc gtcggcctcg gatggccgca
     4261 atgccaggga gaagccgcgg aagcggatct ccatcgggtc ccccagggga gcacggcgct
     4321 gcactctgat ctcggtgccc ggaatgaggc cgagccgcat cagctgggct gcgtagtcgt
     4381 catcctcccg gtagccgctg acgcgggcca tatctccggg tgaaagatcg cgtagtgaca
     4441 cggacaacgg tggcctccgg ttcgggccgt ggggaacggc ccgccattta aataaaaatc
     4501 attctcattg gcaattggca gcaggtgggg cggcagaggg ctcgtccgct tactccgact
     4561 ttccctgctt gcgcacgtaa agcacgagct cgtggtcgat gagctcgaat ccgtgctcct
     4621 gggcgatctc ccgctgcagc gcctcgatcc gctcgttgac gaactcgatg acctgaccgg
     4681 tgtccatgtc caccatgtga tcgtgatgct cgtcgccggc cagctcgtac accgagtggc
     4741 cgtcatcgaa gttgtggcgg gatacgatgc cggcggtctc gaactgggtc agcacccggt
     4801 aaacggtggc cagacccacg gaatcgtctg aatcgatgag actcttgtag acgtcttcgg
     4861 cgctcaaatg gtgcggctcg gcgctctcca gaacctccag caccttcagg cgcggaagcg
     4921 tgaccttcag gccggcacgt ttgatgtcgg atccgggcaa cggctttccc caacggtagt
     4981 tggggacact agcccatgca gggctgaccg tccacccgtg accatggggc gtggagtggc
     5041 tgggttacaa tcgccgtcgg cacggggtaa cgacatcggg aggacgatca cgtggcagat
     5101 ctgaaaggca aggtggcagt ggttaccggt gcgagccgcg gggtaggccg gggcgtagcc
     5161 gaaggcctgg ccgaggccgg cgccaccgtc tacgtcaccg ggcgcagcga gagccacgac
     5221 aacccgcctc aggagcgcac cattcaggcg accgcggcga gcgtcgatgc ccttggcggc
     5281 aagggaattg ccgtgcgggt agatcacaac gaagacgccg aggtcaaagc tctcttcgag
     5341 cgcgtcatga gcgagcaggg caggctcgac gtcctggtca acaacgtcta caagattcct
     5401 gatccgccgg ttttcgatgg cggcttctgg gagcacccgg tatccgtctg ggacgatcag
     5461 tgcggggtgg ggcttcgagg ctactacgtg gcctccgtgt tcgccgcgcc gatgatggtc
     5521 gcccggcgca gcggtctcat cgtgaacatc tcctcacggg gcggcgacca gtacgtgttc
     5581 agcacctcct acggcgtggg caaggcgggg gtcgatcgca tggctcggga catggcccac
     5641 gagctgcgcg gctaccatgt cgctgccgtg tccctgcgac cctcctcggt gaagaccgaa
     5701 ttcattctgg acgcggtggc cagcggcgcg gtcaagctcg acatggccaa ggcgcagtcg
     5761 ccgcggttca ccgggcggtg cgtggccgcg ctggccatgg acccggatct catggagaag
     5821 tcgggcggcg tctacatggt ggccgacctc gcggccgagt accggttcac cgacccggac
     5881 cgcgtggagg cgagctgagg tgcagccggt ggaacgtcac gtggacgttg ccacgcccga
     5941 cgggtccatg aacacgttca tcgcgtaccc ccaggggggc ggtccgttcc ccgtggtggt
     6001 gttctacatg gacgcgccgg gcaagcgcga ggagctgcac gacatggcca gacggctggc
     6061 gtcggccggc tactacgtca tgctgccgaa cctctactac cggcgcgtcc gggagtttgt
     6121 catgaccgcc gaagcccggc cggagatgat ggcccacatg aactcgcttt caaaccacat
     6181 ggcctgcgag gacaccgccg ctctgtttgc cttcgcatcg gcagaccgag cggcgcggga
     6241 tggcccggcc ggctgcgtgg gctactgcat gagcggaccc ttcgcctttg ccgcggctgc
     6301 cgcctttccc gatcgcattg ccgcggccgc ctccattcac ggcgtgaggc tttgcacgga
     6361 cgcgatcgat tctccccatc tgaacgcgga gcacgttggg ggcgagcttt acttcgcctg
     6421 cgccgagcac gacgactggg ctcccccgga gatgatcgaa gccctggagg ggcacctttc
     6481 ccgggtgggc gccagctatc ggatcgagtg gtatccgggc acttctcatg gcttcgtctt
     6541 ccccgcacgg cccgtctacc acccggcggc agccgagcgg cattgggaaa gggttctcga
     6601 tctcttcagc cggaacctgt gaacccggcg atttcctctt ccgtcatgcc ggacacctgg
     6661 gccgatttcc ggatcgcggc ccaggtgttg tcgtcgatgg gtatgccccc ggccgcgcgc
     6721 tcggccatgg actcgagctc gggttccccg ggcacccgca ccctgtcgac gccggcagcc
     6781 ggcatcgtgc tgcgcaggta ggcaatcatc tcctctacct cccgatggaa ggcctccgcg
     6841 ccgccgaaca ggtccgggtc cagcacgacc atgagcatgt ggttgacgat ggtgaaccgg
     6901 tccgggggag gtgcctgggc cgtccactct ccggcgagcc cgccaccgag aagctcgcac
     6961 atcagggcga gcccgtaacc cttgtgatgt ccgaatggcc ccagcgagcc ccgggggtct
     7021 tcgaacatca cgctgggatc gttggtcggt tttccttcgt ggtcgatcag ggatccgtcc
     7081 ggcaccttgt ctcctttcat gtaggccacc cggaccttgc cctgagcaat ggcgctggtt
     7141 gccatgtcga gcaccacagg gggctcgtcg ctcctcggca ccacgcagca gaaagggttg
     7201 gtgctgatgc gcgcctggcg cccgccgaac ggcgagacca gcggttggtg tcccaccacg
     7261 ttgacgaagt gcatcgacac gtagcccgct cgcccgcaga tctcgccgta gtgcccgatg
     7321 cggcccaggt ggtggcagtt gcgcacgccg gccgcgacga cgcccgtctg cctgacccgc
     7381 tcgatggcga gctcgacggc ttcgcgcccc accacctggc cgaaacccat ctgcccgtcg
     7441 atgagcataa cggcgccctt gtcccttacc agcctggcat gggtgttcag cgcgagagcg
     7501 cctgagcgga tattgccgac gtaggtcggc accatgccga ccccatggga atcgtggcct
     7561 ttgagattgg cggacaccag atgggcggcg acctcctgcg cgtcgctggc ttcggcgtcg
     7621 agccgaacga agatttcgct gacgaagcgc gtcagtgcat cgcggtctat gagcatggtg
     7681 gcttctcatc gtgttggaac gctcagtgtt gcctctttaa agagcctgtg acaaggtctg
     7741 acgagagggg gttggcatgg acaagtgcgc ggaagggttc agcctgatcg aagggccggt
     7801 ctgggatccg gaccgcggcc tgcttttcag cgacgtcctg gtcggtgggg tcttctgcct
     7861 cggcggcgac ggcacggtga cgacggtttt cgagcatcgt cggggcatcg gcggcatggc
     7921 cctgcacgaa gccggtggcc tgatcgtcag cggccggaat ctgtctttca agccctacga
     7981 tgagggctcg acggtcaccg ttctcgatcg cgacgaggag aacggcctgg tgggcttcaa
     8041 cgatctcacc accgatgccg aagggcgaat ctacgccggc agtctgggag cgagccccgt
     8101 gttcgccgac gggcgtgagc cgcgtgcggg ggatctctac ctggtcgatc tggacggcag
     8161 cgcccgggtc gtggccgagg acgttcggct gaccaacggg ctggggttct ctccggacgg
     8221 taccacgctc taccactccg agagcgggcg ccgaacggtc ttccgctacg acgtgcagcc
     8281 ggacggttcg ctctcggcaa aggagccgtt cgttacgccg gataacgggg tgcccgacgg
     8341 gctcgcggta tcccgcgacg gcgctgtctg ggtggcgctt gcccggggcg gtcacggcgt
     8401 agccgtctat gaggcgtccg gccgactgcg tgagttcatc gagattcccc tgcccatgtg
     8461 caccagcgtg tgctttggcg gcgaagatct gaaggacctt tacatcgtct ccgggtcggc
     8521 gggcgccggg tctgacaggg cgggagccgt cttccgagag cgtgtcgatg tggccggcct
     8581 gatcatgaca ccggctcggg tgcggctcgc gtgacgaagg cgtcgccgtc gacctgcagg
     8641 caggtgcgtc ttcatcggtt agagcatagg agtttgcagg catggcgata gaacccatgg
     8701 acacgggaac cgaggatctc ctcgcacatc aggaagatgg cgtcgcggtg atcacgttga
     8761 accggcccca ggcacgcaat gccatgtcgg ccgcgatgaa cgcaggcctg gcaaaggctc
     8821 tggacgaggc cgagcggaat cccgccgtgc gctgtgttgt catcaccgga gccggcggcg
     8881 cgttctgcgc cggcggagac gtgaaaggca tggcggcgcg aagccagggg ggcggccccg
     8941 ggcccaccgt ggacgagcgc attcaccttc agcgcctgaa ccagagggcg acggccggcc
     9001 gcatttacca gatgccgaag ccggttttgg cgagccttcc cggaccggct gcaggagcgg
     9061 ggctggctct cgccctggca gccgatctgc gcatcatggc cgactccgcc ttcatcacga
     9121 cggccttcgc gaaggtgggc ttcagcggcg acttcggtgg cagcttcttc ctctctcgcc
     9181 tggttggcag cgggaaagcc aaggaactct actttctttc cgatcgggtg agtgccgctg
     9241 agtgcgagcg gctgggaatc gccaacgcgg tggtgccggc ggaagaactc gaggcccaga
     9301 cgatggcgct tgcgcggcgg ctggcgtccg gaccgcccgt ggcataccgg tacatgaagg
     9361 agaacttcaa ccgggccgcc gcaggtgccg gggtgctcga gtgcctcgac ctcgaggcca
     9421 cgcatcacgt gcacaccggt ctcacggagg atcatcgcgc ggcggcaaag gccttcgtcg
     9481 agaaaaccga ggtcgagttc aagggccgat gaggcgttcc tccaggagcc gcggcgctcg
     9541 ggcgcgtcct cagcccggtg tcagaccccg ttcggccagc cactcggggt tgaacagtcg
     9601 ggactggtac ttggcaccgg tgtcgcaaag cacggtgacg atcgtgtgtc ctggccccat
     9661 cgagtgggca acctgaacgg cggcgcacag gttgatcccc gtgctcgaac cgaaaaacca
     9721 accctcctcc cgcagctcgc ggtagaccat gttcacggcg tcccggtcgg tcacctgaac
     9781 cgcgtcgtct atgcaggtac cggccaggtt gtccgttacc cggctgttgc cgatcccttc
     9841 ggtgatggac gggccctggc tcatggtggt ctcgccggtg gttacccagt tgtagagggc
     9901 gctgcccatg gggtcggcga gaacgatgcg aacatcctcg cgtcgttcct tgagaaagcg
     9961 gctcacccct gccagcgtcc ccccagtccc ggtggcgcag gtgaaagcgt ccacccgtcc
    10021 ctcggtctgg tcccagatct ccggccccgt ggactcgtag tgcgccaggc ggttggcgac
    10081 gttgtcgaac tggttggccc acaccccgtt ttccagggtc tcggcgcagc gtccggcctg
    10141 tttctggtag ttgttctcgt cgcggtaggg aaccgcaggc acctcccgga cctcggcgcc
    10201 cagggtcttc aagagctgaa ccttctccgg ggattgggtg tcgggcatgt agatgatcga
    10261 ccggtagcct ctggcgttgc agacgtgcac caggccaatc ccggtgttgc ccgcggtgcc
    10321 ttccaccacc acgccgccgg gcttgagcgc gccggacctc tcgtgctcgc ggaccatcca
    10381 cagcgcggcc cggtccttga ccgagccgcc aggattcagg aactctgcct tgcctaggat
    10441 ctcgcagccg gtctcttcgc tcagccggtt catgcggatc aacggggtgt tgccgatggt
    10501 gccggcgaag ccatctttga tgtccatggc cgattttagt caggatccga ttgaatgcgg
    10561 aagttgccat gggtgatcat cccggctcgc gggcaagttc aggagatgcg cacagacatg
    10621 aagaaggccg atctgcagga ccgctggctg ggcgatgcgg gggaaaacgg ggtcaacccg
    10681 ctgcgcatcg ccgcgctcat ggcgcgggtg cgcaaagagg tggacgaggg gctgctgccc
    10741 gcggcgcagg ttgccctggc cagaaacggc gccctggtgc tgttcgaaac gatgggcgct
    10801 gcggacaacg actcgctctt ctgcgtcttc tcggcaacca aagccatcac gtcggccgcg
    10861 gcctggatcc tcatgcagga ggggcgcctg aaggaggatg agctcgtagc cgacatcatc
    10921 cccgagttcg ccgcgaacca taagcacgat atcaccgtgg ggcagctgtt cgtgcacacc
    10981 gccggctttc cgtccgcgcc cttcaagccc ctggactgga acgaccgcgc gcgccgtttg
    11041 gagcgttttg cccaatggca gctgaactgg ccgccgggca gccgctacga gtaccacccg
    11101 acctcctcga tgtgggtgat cgcagagatc atcgagcggc gcgcgaatac cacctatcag
    11161 gagttcgtcc gcgaacgcat cgccctaccc ctggggctgc ccgagctcta cgtggggctc
    11221 ccggcgaccg agaacgagcg ggtggtgccg aacgtgcatg tgggcgatgc cctgacgcga
    11281 gcggactacg agcttctcgg tctgccggag ccgcccgtga ctgaggtgac cgaggaagcg
    11341 atcctgaagt tcaaccggcc ggaagtccgc gcggtcgggg tcccgggcgg gggcgggatc
    11401 atgagcgcag cggatctggc gctgttctac cagggattgc tgcatggtgg cctgggcgag
    11461 gaagtctgga gcgccgccac ccttgccgac gcccgtcgcg tgcgcagcgg cgaactccgc
    11521 gacccggtga tgggccaccg cgccaatcga gctctgggcc tcgtcatcgc gggagatccg
    11581 caacggaatc tccgcggttt cggcaagacc aactccccgg agaccttcgg ccacaacgga
    11641 gccggcgggc agctcgcctg ggccgatccg gcgaccgggc tgtccctggc ctactgcacg
    11701 gccggtcacg atcgcaaccg gatacgccag gggaggcggg gcgtagcgat ctcgagcctg
    11761 gctgccgtgt gctgcgaaga ttgaccagga cgcaacaccg cgcgcaggaa ccgcttgatt
    11821 tgcttctcaa tctgctccct ggcgtcgggg agcgcggaac gaactgtgac agacgcctct
    11881 gtccccgcag ggcggctgaa ctaatctgcc gactacgctg attgccaccg gcacagcgag
    11941 acgcgagagg agtcaccggc ccgtgcggcg gtgaacttaa cggttacacc gggattcgct
    12001 gtcgattctt cggggcttcc ccgacgggtc gagcagtcgg cgagttccgg ttttacatcc
    12061 ggcgtcgacc cgatgtccgg gtcaaggatc tcccggcatc agcccgaccg ccaggacggc
    12121 ggaggcgaac agagcaagga ctgcggaaac ggtggttgcc ggtagttcga aggatttagc
    12181 cgaaaggatt tcggctccga agggacttcg gagtggtgca aatggatctc aggagcatgg
    12241 agcacccaat ctttcgacgg cacactgttg tctcccccgc gtctgaaagc ggcgcatccc
    12301 cgcgcctcat ggggttacca acgggttgat ggtgcggatc tcgcaatgtt cgccatcggc
    12361 ggtgatggcg tcgaacccgg cgtagatggt gttgtttgac atggtcacgg acacgtttcg
    12421 cgcgaatccc agaccgatgc atccctggcg cagggtcagc aggtatcgcg tctgcggact
    12481 gacctccagc acgacccgct tgtcgtccac tcttgcccag ccttcgatcc ggttggctgc
    12541 cgggatgctc gagacggggg cctgcacagt gccctcgaca atggactccg cgcgggggcc
    12601 aatgggagag tccggcggca gggcgagtcc ggacgacgcg tagagcagta ctgtcccggc
    12661 gattgagcaa gcaagttttg caaatttcga ggtgctgggc atgacgtttt cctctggcaa
    12721 gggcgacgtt caggctatga gcgcgacgct gaatgtcggc tgaacatgag gcaacggtac
    12781 gggagcccgt gtgaaaaggt cgtgagaccg cttgaggggg ctgatgcggt tcgagggttg
    12841 gcaacgccgg ggatattccg caaagtgtcc acgaggcgaa ccagcactgg gggtacggtt
    12901 tgatcgatcg gggagcacat cggcgccgcg ccgcaatcct gaatgaggcc gcccggcgga
    12961 tcgttctgct caacctggcg atattcagca tggccgcgtc ggccgagtcc gtcctcgagc
    13021 gggtcgatgc ggaagccgcc gccgccgtat cgatggccga gcgcctctgg gcgctggctg
    13081 agctcggcta tcaggaaacc cgcagctccg agctgctcgg ggcctatctt gcagagagcg
    13141 gattctcgct cgaaagcggc gtggcgggga tacccacggc ctttgtcgcg acattcggtt
    13201 ccggccggcc cgtcatcggc gtcctcgcgg agttcgacgc gctgccgggc atgtcacagg
    13261 ccgcggaacc ctccagacga cccctggagc ccggcggggc cggccacgcc tgcgggcatc
    13321 acctgttcgg cgcaggggcg gcagcggcag cggtttccgt ggcgcgctgg atggaggcaa
    13381 acggggtgcc tggctcgatt cgcgtgtacg gcacgcccgc cgaggaaggc ggttccggga
    13441 aggtctacct caccagggca ggccttttcg acgacgtgga cgtcgtgctc cactggcacc
    13501 ccggcagccg caacgacgcc tcgccggcaa ccaccacggc gaacaagtcg ggccgcttca
    13561 ctttccgggg agtggccgcg catgccgccg ctgccccgga gcgcgggcga tccgctctgg
    13621 acggcgtgga gtccatgaac tacatggtga atctcatgcg cgagcacgtg ccgtctgaca
    13681 gccgcatcca ctacgtgatc accaagggtg gcgatgcgcc gaacatcgtc ccggaaatcg
    13741 cccaggtcta ctactacgtg cggcatccgg aggcggccac cgtcatcgag ctttttgagc
    13801 gggtcgtcgc ggcggcgcgc ggcgctgcaa tgggcaccga gaccgacgtg gactacgagg
    13861 tgatgcacgg caactatgcg gtgcttccca acgaaacgct ggccaaacgg gttcacgccc
    13921 acctcctgag tctcgggggc gtgagctact cgcccgatga gcgccgattt gccgagcaga
    13981 ttcaggcgac cctgggagac tcgctgccgc cgctcagccg tgccgcgagc gtcgagccgt
    14041 ttcagatgcg gcagaacatg ggctccacgg atgtgggcga tgtgagctgg aacgtgccca
    14101 ccgtcggctt ttacgcggcc acctgggtac cgggcacccc cgcgcacagc tggcaggcgg
    14161 tggccgcggg cggaacgagc atcggccaca agggcatgtt gctggcgacc aggaccctgg
    14221 cgctcaccgc ggcggacctg ctcaggaccc cctcgctcat cgcggcggcc cgggcggagc
    14281 tcgaagagcg tcgcggccag gacttccgct acgttgccct gctcggcgag cgcgatccgc
    14341 ctttggatta ccggcggtga acctcggggt cctgcacccc ggggagatgg gcgtcagcgt
    14401 gggcgccgtt ctgacggccg cagggcaccg ggtgtactgg cttcccgagt ctcgcagccg
    14461 cgcaacccgc gagcgcgccg aaggcgccgg cttcaccggc tgcgcctcgc tccgggaact
    14521 tgcggaccgc gttgccggca tcgtcagcgt ctgtcctccg gcggcagccg aagaggtggc
    14581 gagccaggtc cgctcggcgg gcttcaccgg cgtctacgtc gatgccaatg cggtgtctcc
    14641 cgccacggcg aggcggatgg ctcgaacgat gggtcccacg tacgtcgacg gcgggatcgt
    14701 cggccctccc gcggtccgtc ccggccttgc gcggctgtac ctgtctggag ctgcggccga
    14761 ggaagtggcg ggatggtttc atggcggcct tctcgaggcc cgggtcctgg cgggcgaggc
    14821 gggcgcggcg tccgcgctga agatgtgtta cgcggcatat accaagggca gcagcgctct
    14881 tgtgctggcc atccgggcgc tggccgaggc cgagggggtg gacgagccgc tgttggccga
    14941 gtgggacatc tctcagcccg gtcttgccga tcggagccag cgtgcggccg cgggcacggc
    15001 ggccaaagcc tggcggttcg tcggcgagat ggaggaaatc gcggccacct ttcgcggggt
    15061 gggattgccg ggcgagtttc acgaggcggc ggccgttgtc taccggcgtc tggcaccgtt
    15121 gaaagggaac gcggcggcag aactcgccga cgtggttcga cggctcatcg agaatcccgc
    15181 ggatgtcggg gaagattagc cgcatcgccc ggatggccgt gtcgtgggcc atcgcggccc
    15241 cggccatcgc ggccccgggc tatctgttcg acgaggcggc cacgctcgac ctcgagctgg
    15301 aggcccccct cggagcgctc atgggtgacc gctacccaga cagcgagtac cggcctgcgc
    15361 agctccgcta cgtgggcaag gacggtagcc gcgtcgcgct gcccgttgag gtccggacac
    15421 gtgggaaaac ccgccgggac gaggaagtgt gcaagatgcc gccgctgcgt gtgcgcttcg
    15481 atcaagccgc cgcggacggt acgccgttcg ccggtcagcc cgcactaaag ctggtcactt
    15541 actgtcgcga cagcgactcc ttcgatcagc tcgtgctgct cgagtacctt gcctacagaa
    15601 cctacaactt gctgaccccg cagtcgcatc gggtccggct tgcgcgcatc agctacctcg
    15661 agcgtgggga ccggcgaacg acccgctacg gcatcttctt agaggactgg cgggcagtgg
    15721 cagcccgcaa cgacctcacc gccgacccgg tcgacggcgg tgtgaacgtc gacaagctca
    15781 accacgacgc cgccaaccgc gtggcggtct ttgcgtacct gatcggcaac gaggactact
    15841 cactgctctg gccggaaccc gacgataact gctgccacaa catcaagccg ctgctcgacc
    15901 gggatggccg ggtggtgccg ctgccctacg atttcgacta ttccgggctc gtcaacgcgc
    15961 cttatgccgt ggccaagaat ccgcgcagaa aagttcgcca gcgagcctac cggggtcttt
    16021 gcgacaccca ggaaggtctc gaggcagcgc tggagctcac gcgtgcgcgg cgcgaggaca
    16081 tctacgcggt cttccgcgcc caggaagggt tgtcgccggc aaagctgaag tcgtcaatgg
    16141 gctatctcga ccggttctac gacttgatcg accagcctgc gcaggtcgaa aaacgcctgc
    16201 gccgtcggtg cagaaaaagc tgaacgatca ggcggggtcc ggcgcgatgg gtatctctgc
    16261 agggacatcc gcggcggacg gggcgtcacc ggagtcgacg tcgattccgg atcccgccgg
    16321 ctcgccgcag tcccagccca tggcgccgag cttgtctgcg aaagcggccg cctgggcctc
    16381 gcagtagccg gcttcgttgg atgcccgcca cagcgagtgg attcccgcgc cttcctgagt
    16441 cttgtcgtag atcacttcgc agggaagggt ctggtcggga ccgctgtaga cgacctcgac
    16501 gctgcgaacc aggctcccct gagtgcattg caccggattg gcgaacgccg gagcggcgat
    16561 ggccgagacg atgaggatga cggtcagttt cgttgctggg tatcccatga gttgctcctt
    16621 gagatcacgg ccggaaccag aaggtcggcg gatcggttct cagtgcctgc cgcgccagct
    16681 tcatccatcg gcacagcgcg gggcgggttt cgcgggggtc tatgatctgc tcgatctcga
    16741 agtgctccgc agaacggaac ggcgagcgca gccggttgag gcgctctttg atccggctga
    16801 ggtgagcatc gtaatcgtcg gcctgggcga ggtcggcctt gtaggccacc tcgatgccgc
    16861 cctcgatggg aagcgagccc cagtctcccg acggccacgc catgcgtacg caatggctgc
    16921 cgggtttgtg gttggaagcg ccagcgaccc cgaacgcctt gcgcaccacc acgcagcaga
    16981 aggggacggg tgcctggccc agagcggcca gcgcctggca accgaagcgg atggttccgc
    17041 tctcctcgga ctgcttgccg atcagaaatc cggggcaatc ctccagatgc accaacggca
    17101 ggtggaacgt gctggccaga tcgatgaaac gcaccagctt gcggctcgca tccgccgtcc
    17161 acgcgccccc gtacaccatc gggttctctg caaaaagggc caccggcatg ccgttcagcc
    17221 gggccagacc ggtgatgatg gatcggcccc aacgcctgcc gatctcgaag aacgacccct
    17281 cgtcgaccac cgagtcgatg atccggtgca tcttgtagac cttgcgcgga tccttcggaa
    17341 cgatctctag cagcgcctcg tccgtccgct cgggatcgtc cgtggcgtcg gtgacgggcg
    17401 gcagctcgtc cgtgctggtg ggcaggtagg agagaaatcg ccgtgccatg tcgaacgctt
    17461 cttcctcgga agacgcctcg tcgtcgatgg ccccgtttcg ggtatggatg cgagaggcgc
    17521 cgagctcttc cttgctgaca tcgccgaggc ttgcccaatc caccagtgcg ggtcccgcga
    17581 tcatcatctg ggcgctgttc ttgacgatga cggagtagtg gctggtcgcc acccgggcag
    17641 cgccgatacc ggccacggag cccagcgcca gagagaccga tggggcaacc gccaggtgct
    17701 gaacgacggt ctcccagccg cgcacctcgg gtatgtaggt tcggccggcc atctcgatgg
    17761 tcttcacgga tccgccgccg cccatgccgt ccaccagacg cacgtgggga agccggagct
    17821 cggtggccag gccctctgcc tggagccgtt tgccgatgat cgacgcgtcc gcggagccgc
    17881 cgcgcaccgt gaagtcgtcg cctgagagaa tcaccggccg gccgtcgatc tccgcggcgc
    17941 cgaatacgaa attggagggg gtgaacccga tgagattgcc gtcgtcgtcg tactgcccga
    18001 cgccggccag ggtgccgatc tcctggaaag agccggagtc gctgatggcg gcgatccgct
    18061 cgcggatcgt cagcttgttg aattcgtgct ggcgcgccac cttctcggcg ccgcccatct
    18121 gataggcgag ctgctcgcgc ttgcgcagct cgcgaatttc ctcctcccag ctcatgtggt
    18181 cggcgtccgg ctgcggtggg cgaagcgcca cgcagccggt tcgctcgggc tcggatccgt
    18241 caggcgcggg ttccggtaaa cgtggcgtcg ccgaaagcgc cgagcttgac gtttccggtc
    18301 atctcgtcac cgtttacggt cgcgctgaac tccagcgtca tcgccatggg cgtcgtgatg
    18361 tcagccttcc aggccagcga gtcgccgtct gccgtgccgt ccttgagctc gatctcgccc
    18421 tggggtcctt tcagcgtgcc cgtcagggtg ccgccgtcgg tggccagggt catggtgccg
    18481 ttctgggcgc ccatgggcgt gttcatcacg gtgttccagg tgccgtctgc gctcatgtgt
    18541 ccgtccctcc agtcgttgca gttgagggct cggagtatgg catagcgggc ctggtagaca
    18601 aacattccgg agcctgagtt aatggccgcc cgtgagcatc ggcaccctca acgaagggcc
    18661 tctgcaccag gcaatcaagt cgctctacaa cggcgccggc gcccgtgagg aggtgccggt
    18721 cggcgacttc gtagccgacg tcttcggccc cgacgaggtc atctacgaga ttcagaccgg
    18781 cggtttcggc agcctcaaga agaaactcgc tcgcctggtg gaggatcatc gcgtcgtcct
    18841 ggtccatccc atcgcagagg tgcgctacat cgtcaagctg ccccaggggg aagaccagca
    18901 ggccagccgt cggcgctcgc ccaaacgcgg cagcgttgcc cacgtgctcg accggctggt
    18961 gagcattcct gcgcttctga atcatcccaa cttcgagctc gaggttctgc tgatcgagga
    19021 agaggagttg cggaccttcg atccgcgcag ggtccgccga cgggatggat ggcgtgtggt
    19081 gcagcgccgt ctgggcagcg tgctcgcctc cgagcgtttc acctgtgctg gcgatcttct
    19141 gcgcatggtg cccgggccgc ttcctgcgga gttcaccacc gaggatctcg ccggcgcgat
    19201 gggtcagccg cgctggcttg cccagaagct ggcttactgc ctgcgcgagg ccggagagat
    19261 cagcatctcc ggcaagcggg gcaacgccct gtgctaccgg ctagcgcgtt cgggcgccgt
    19321 ggcttgacat gcggcccggg ggcctcgaat actccttcgc ccgcccatca gcgagcatac
    19381 ccttgatcga gctttacacc gcgtctacgc ccaacggctg gaaagcgtcc gtcactttgg
    19441 aagaactcgg ccttccctac gaggtacggc ccgtcgacct gatggccggc gagcagaagg
    19501 ccccggagtt tctgaagatc aacccgaacg gcagagttcc cgccatcgtg gacgacggtt
    19561 ttccgatatt cgagtccggc gccatcatga ttcacctggc tgagaaaacc gggcgcctca
    19621 tgccggctga cgctcatgga cgttctctgg tcatccagtg gctgatgttc cagatgggcg
    19681 gcgtcggccc catgatgggt caggcgaacg tcttctatcg ggcaatggag gaaaagatcc
    19741 agcccgccat cgaccgctac cagaacgagt gtcggcgctt gttcgaagtt ctcgatggcc
    19801 gtcttcggaa cagcgagtgg ctggccgacc acttctcgat tgccgatatc gccaactggt
    19861 gctgggcgcg cctttactac tggtcgggtg tttccctcga cgggctcgac gccatgcagc
    19921 ggtggatggc cgccatggag gcgcgtcccg cctgtcagag aggcgttgag gttcccaacc
    19981 ggctcgagct gcctcgagac gatgatccgg acgggatcga acgccaggca ctccagtcac
    20041 gccgcgtgct ggtgcgctga tggccctcga atcggtaatc gaagggccgg cctgggatct
    20101 gacgggcgag tactcgtcgc ccgactctca gggcttgcag gccgacctcg accgactcag
    20161 cgagctgctc gatcggatcg aggcgctcaa cgggcag
//
LOCUS       DBNHKV010000076        10197 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1216618, whole genome shotgun sequence.
ACCESSION   DBNHKV010000076 DBNHKV010000000
VERSION     DBNHKV010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10197)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10197)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10197
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1216618"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="AC16PP_02250"
     CDS             <1..695
                     /locus_tag="AC16PP_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6480474.1"
                     /translation="YSEISDLQTARFDPSNVAFLVFLENVGDAEIKGFDADFTWLATD
                     RLTVSGAFSIVDTEITRLNPQLQGIAVPEGSELPFAPNFSGNIRARYQFPLDAFEANG
                     YVQLGVSYRGESLSGITGNAFLAEQVHQEVYGVGSGLKIKSEGGTYSSPIPATGETFE
                     NGRYKQESYALVDLAVGVQKNGWTAEFFVKNLADESAQIHITTSNSADFTPKVVTNRP
                     RSFGLRVSYDHF"
     gene            712..2712
                     /locus_tag="AC16PP_02255"
     CDS             712..2712
                     /locus_tag="AC16PP_02255"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     sulfotransferase family protein"
                     /protein_id="HIR6480475.1"
                     /translation="MSVASSGPGPDHREPSLASARRALEQGQFDAAVQQAEALLGRGD
                     ADTSAARYLLAVACRYAGDCERAEAALQELLASEPRHARAWQERGYVALKRNDPNAAR
                     EYFRHAVTHNSALVASWRSLVKLHGAAGERELAEQAARRLDELSALPREILAAASHLN
                     EDRLYKAEQICRDYLQENPTHIPAMRLLAEIGVRLQVLDDAEFLLESALEFEPDHELA
                     RADYANVLIRRQKFTKALDALASLVAQRPEHLPYRIAKAMATAGAGDHEAAIAQYRQI
                     LEDHPDLPQLHVSLGHAQKTIGDMNGAVASYQQAYGVRRDFGDAYWSLANTKTYRFTP
                     DEIDGMRQAEADPTCGTQDRIHLCFAAGKAFEDAGRYEESFAYYERGNRLKQGEIRYD
                     AGQIDRRVEAQMAVCTRELFARQEGHGDPAPDPIFVVGLPRAGSTLLEQILASHSMVD
                     GTMELPSLLAIAQRLRGRSSVRSDRYPRILGELKPEQLHQLGAHYLQETLPYRQGAPR
                     FVDKMPNNFFHVGLIRLLLPNARVIDARRHPMACCFSGFKQLFGEGQEFSYGLTEIGH
                     YYRRYVELMKHWDEVLPGFVLRVQHEDVVDDLEGQVRRILEFCELPFEDACVNFHRTE
                     RAIRTPSSEQVRQPIYRDGLDQWQRFEAWLDPLKAALGDALG"
     gene            2768..4750
                     /locus_tag="AC16PP_02260"
     CDS             2768..4750
                     /locus_tag="AC16PP_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HIR6480476.1"
                     /translation="MQSGDKAYAELDRPVFLTSASLLAAVIFLAALFPDTMDALFSDV
                     QGAIVAGASWYYVLVVAVILVSVLVFAFSSYGQIKLGPDHSEPDYSFISWFAMMFAAG
                     MGIGLMFFGVAEPVMHFLSPPKGDPGTIAAAREAMKLTFFHWGLHGWAIYAIVALILA
                     YFSFRHGLPLTLRSALYPLIGERIYGPIGTAVDVFAIISTTFGVATSLGLGVNQINAG
                     LNYLFGLPVTQEIRVGLIIVTTAIATVSVVLGLDRGIKRLSEINIVLAVMLMITVLAI
                     GPTTLLLQAFMQNTGDYLADLVGKTFNLYAYDPTDWLGGWTIFYWGWWISWAPFVGLF
                     IARISRGRTLREFVIGVLIGPVVFTFLWMTVFGNSAIDLIMNQGVTELGSAVQANESI
                     ALFRFLEQFPATGFLSALAIVMVVVFFVTSADSGALVLNMLSSNGSDQTPVGRRIFWM
                     SAIGVTAIVLLLVGGLSALQTAVIASALPFSVAVMAGIWGFSKALYVDHAKRVSHAAA
                     TVPPILARPEISWQHRLTQLLRHPQDAEVRAFMRERVMPVMREVGDELKRHGLDVEVI
                     VDGGEGESLRLEIAHGEEIDFVYEVVNRKHPLPDEALVGTAMKDMPPSNTYHRAEVHL
                     REGSQDYDIMGWTSEQIAHDITEQYEKHRHFLHMLR"
     gene            complement(4762..5922)
                     /locus_tag="AC16PP_02265"
     CDS             complement(4762..5922)
                     /locus_tag="AC16PP_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6480477.1"
                     /translation="MENVYRNGLMAILSLLLLGACGGEATQTNEVVFGVHTDLSGPTA
                     IWGVGATNGARMRFDEANAAGGVHGRSIRYVVEDAGYQVPNAIRAANKLINRDQVLAM
                     VLAVGTPMNNAIMMEQFEQGVPNLFPISGARSMVEPLHPLKFTQRGIYYYEVRAAIRY
                     FVDSQGKQSVCAIYHDTDYGHEILEAVNDQLAEMNMALVESAAHKPSESEFTAAILRL
                     RNAGCDLVLMGTVHRDTILVLDSARKMGWEDVAWVGNNAAYGQVIADSPSCEGYYAFV
                     HMAKIYADDEKTPAVQAWWDHYVALYDEEPGLAAMEGYRAADLLVLALEKAGPDLSVG
                     SLLAALEGITDYTDLFGYNVSFGPEKHSGATESVLSQVQGGRWVTLAESISY"
     gene            complement(5951..7765)
                     /locus_tag="AC16PP_02270"
     CDS             complement(5951..7765)
                     /locus_tag="AC16PP_02270"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR6480478.1"
                     /translation="MSPQIIDGCATVARLFWKRATDLPDKIALREKDFGIWNEYTWGD
                     WGEQARLVGLGLKALGLKRGDVCSIATDVNKEWLFADHGVIGVGGIVNGVYPTDAPNQ
                     VEYLINDSRTRFYFAEDEEQLDKVLEVRGNTPTLEKIIIFDMEGLRTFSDEQCMSFDA
                     LLALGREYGEQHPGLWEQEIEAAEAEDLLILTYTSGTTGNPKGAMISNRNMIFMMRNL
                     QRMYGITSADEQLGFLPTAHVAAQMFYNFAVIESCSVINLVEELETTDRDQQEIAPTI
                     HFAVPRVWEKKHSLVTIKLKEGTVLGRWAYDLAMRIATPLAHARQRGETPSWISRLLY
                     RAVDPLVLKNIRVLLGIDRCRWLSTGAAPIAPELIDWYWALGRPMFEVYGQTECTGLA
                     TANHPGEVRIGSVGKPGQEIEVTLSEAGEILIRSPGVICGYWENPEKTAETFRQGWLH
                     TGDVGRIDEDGFVYVVDRMKDIIITAGGKNITPSEIENQLKFSPYITDAVVVGDRRPY
                     LTCLIMIDHENVTKYAQDHDVPFTNYTSLCHAREVQDLVEAEIEKVNARFARVETIKK
                     FRLIDQLLDEEDDELTPTMKLKRKVVHEKYGELIDTMY"
     gene            complement(7762..8520)
                     /locus_tag="AC16PP_02275"
     CDS             complement(7762..8520)
                     /locus_tag="AC16PP_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6480479.1"
                     /translation="MLELKNVETYYGPIMAIRGVSMEVPEGKIVSLLGANGAGKTTVL
                     KTISGAMDPQKGTITYQGEPIQARDPDWVARRGIAHVPEGREVFPFLCVEDNLRMGAF
                     SRRDAEIGDDIELVYGYFPVLKELARQQAEYLSGGQQQMLAIGRALMLRPRLMLLDEP
                     SLGLSPRLVAEIAEIIVRLNREQAVTMLLVEQNAKMALDIGDYGYVMEIGRIVMEDTC
                     ERLKNATDIKEFYLGMKDEGVRGRRRWKQRKTWR"
     gene            complement(8532..9290)
                     /locus_tag="AC16PP_02280"
     CDS             complement(8532..9290)
                     /locus_tag="AC16PP_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6480480.1"
                     /translation="MSLFAAEDVAVSFGGIKAVHDLSFAVEDGEVFSIVGPNGAGKTT
                     IFNLISRIYDVDQGSLAFDGTEITRLPAHAVAELGVARTFQNTELFEQETVLKNLLIG
                     CHVHRRSGLLADILFLPSVKAQELTFRKRVEDVIDLLDLQSYRDQVIGNLPYGVRKMV
                     EIARALCLGPKLLLLDEPSSGLNPEETEDLSFWLEDINNDLGITIIMVEHDMNLVSRA
                     SDRVMALADGALLTIGSPADVQQHPDVLRAYLGD"
     gene            complement(9287..>10197)
                     /locus_tag="AC16PP_02285"
     CDS             complement(9287..>10197)
                     /locus_tag="AC16PP_02285"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6480481.1"
                     /translation="GIGLMLLVGYTGLVSLGHAAFLAVGAYCHSWLLGNGVPLPLAMV
                     AATLVTAGVGAVVGVPALRMTGIYLAVATLALAVIVEKVLEKWETVTGGLRGLPVPKA
                     QFLGFDLGAPANFYYLCLGVLALGLLAAMNLLRSPTGRAMVAVRDSETSAQSMGIHIN
                     RIKGLAFALSAGFTGLAGALFAHRIGYLFPDSFTVLTSIQLLLMVVVGGLGSLHGVVF
                     GAIFIGLLPQGIALFRDVVPPAIAQIPGLEPGIFGLILVLFLIFEPLGIYGRWVKIRL
                     FFEEFPLYRRATYKRQKTYLRTERLR"
BASE COUNT         1800 a         3355 c         3223 g         1819 t
ORIGIN      
        1 actactcgga gatcagcgac ctgcagaccg cgcgcttcga cccgagcaac gtggccttcc
       61 tggtcttcct ggaaaacgtg ggcgacgcgg agatcaaagg cttcgacgcc gacttcacct
      121 ggctcgcgac cgaccggctg accgtctccg gggcgttcag catcgtcgac accgagatca
      181 cccggctgaa tccccagctg cagggcatcg ccgttcccga gggcagtgag ctgccgttcg
      241 ccccgaactt ctccggcaac attcgcgccc gctaccagtt cccgctggac gccttcgagg
      301 caaacggcta cgtgcagctc ggcgtgagct accggggcga gagcctctcg ggcatcacag
      361 gcaacgcgtt cttagccgag caggtccacc aggaggtcta cggcgtgggc agcgggctga
      421 agatcaagtc cgaaggcgga acctacagct cgccgattcc cgccacggga gagaccttcg
      481 agaacggtcg ctacaagcag gaatcctacg cgctggtaga cctggccgtg ggggtgcaga
      541 agaacggctg gacggcggag tttttcgtga agaacctggc tgatgagtcc gcccagattc
      601 acatcaccac gagcaactcg gcggacttca cccccaaggt ggtcaccaac cggccccgca
      661 gcttcggcct gcgcgtctcc tacgaccact tctagggggc aagggcgggc cgtgagcgtc
      721 gcgtcgagcg ggccgggccc cgatcaccgc gagccgagcc tggcatcggc tcgccgcgcg
      781 ctggagcagg ggcagttcga tgccgcggtc cagcaggcgg aagcactgct cggtcgcggc
      841 gacgccgaca cctccgcggc acgttacctg ctggccgtgg cgtgccgcta cgctggagac
      901 tgcgaacggg cggaggccgc gcttcaggag ctgctggcgt cggagccccg gcatgcacgg
      961 gcctggcagg agcgggggta cgttgcgctc aagcgcaacg atccgaacgc cgcgcgcgaa
     1021 tatttccgtc acgccgtcac ccacaactcc gccctggttg ccagctggcg cagcctggtg
     1081 aaactccacg gagcggccgg agaacgcgag ctcgccgagc aggccgcgag acgcctcgac
     1141 gagctgagcg cgcttcccag agagatactg gcggccgcca gtcatctcaa cgaggaccgt
     1201 ctgtacaagg ctgagcagat ctgccgggac tacctgcagg agaacccgac ccacatcccg
     1261 gccatgcgtc tgctggcgga gatcggcgtg cgcctgcagg tactcgacga cgccgagttt
     1321 ctgctggaaa gcgcgctgga gttcgagccc gaccatgagc tggctcgagc cgattacgcc
     1381 aacgttctga ttcgcaggca gaagttcacg aaagctctgg atgccctggc ttcgctggtc
     1441 gcgcagagac ccgagcacct gccctaccga attgccaagg ccatggccac cgccggcgcc
     1501 ggcgatcacg aagccgccat cgcgcagtac cggcagatac tggaggatca tcccgacctg
     1561 ccgcagctgc acgtgtcgct cggtcacgcc cagaagacga tcggcgacat gaacggcgcc
     1621 gtcgcaagct accagcaggc ttacggggtt cgccgcgact tcggcgatgc ttactggagc
     1681 ctggccaaca cgaagaccta tcggttcacg cccgacgaga tcgatgggat gcggcaggcg
     1741 gaggcggatc cgacgtgcgg cacgcaggac cgtattcacc tgtgcttcgc cgcgggcaag
     1801 gccttcgagg acgcaggccg ctacgaggag agcttcgcgt actacgagcg cggcaaccgc
     1861 ctgaagcagg gcgagattcg ctacgacgcc ggccagatcg atcgacgggt ggaagcgcag
     1921 atggcggtct gcaccagaga gctgttcgcc cgccaggagg gtcacggaga tcccgcgccg
     1981 gacccgatct tcgtcgtcgg gctgccgcgg gcgggctcga cgctgctaga gcagatcctc
     2041 gcctcgcact ccatggtcga cgggaccatg gagctgccgt cgctgctcgc cattgcccag
     2101 cgcctgcgag gccgcagctc ggtccgctcg gatcgctatc cgcggatcct cggcgagctg
     2161 aaaccagagc agctccacca gctcggcgct cactacctcc aggaaaccct gccctaccgg
     2221 cagggcgccc cgcgcttcgt cgacaagatg cccaacaact tttttcacgt cgggctgatc
     2281 cggctgctgc tgccgaatgc cagggtcatc gacgcgcgcc ggcatcccat ggcctgctgc
     2341 ttcagcggct tcaagcagct gttcggcgag ggccaggaat tcagctacgg gctgacggag
     2401 atcggccact actaccggcg ctacgtcgag ctgatgaagc actgggacga agtgctgccc
     2461 ggcttcgtgc tcagggtgca gcatgaggac gtcgtggacg acctggaggg ccaggtccgg
     2521 cgcatactgg aattctgcga gttacctttc gaagatgcct gcgtgaactt ccaccgcacc
     2581 gaacgtgcca tccgcacgcc gagctccgag caggtacgcc aaccgattta ccgcgatggc
     2641 ctggatcagt ggcaacgctt cgaggcatgg ctggatccgc tgaaggcggc gttgggcgat
     2701 gcgcttggtt gaattttcga gatggaacga agaaggtggg gcccctttgg tcgcaagcgg
     2761 gacgtgaatg caatcgggtg acaaagctta cgccgagctc gaccgacccg tattcctgac
     2821 ctccgcgtcg ctgctcgccg cggtgatctt tctggcggca ctttttcccg acaccatgga
     2881 cgccctgttc agcgatgtgc agggcgccat cgtggccggc gcgagctggt actacgtgct
     2941 cgtggttgcc gtcattctgg tgagcgtgct ggttttcgcg ttctcgagct atggccagat
     3001 aaagctcggg cccgatcact cggagcccga ttattccttc atctcctggt ttgccatgat
     3061 gttcgccgcc ggcatgggca tcgggctgat gttcttcggc gtcgccgagc cggtgatgca
     3121 ctttctctcg cccccgaaag gagacccggg caccattgca gccgcccggg aggcgatgaa
     3181 gctcaccttc ttccactggg gtctccacgg ctgggccatc tacgcgatcg tggcgctgat
     3241 cctggcgtat ttcagcttcc ggcacggtct gccgctcacg ctgcgctcgg ccctgtaccc
     3301 gctgatcggc gagcgcatct acgggcccat cgggaccgcc gtcgacgtct tcgccatcat
     3361 cagcaccacc ttcggcgtgg ccacctccct cgggctcggc gtaaaccaga tcaacgcggg
     3421 gctgaactac cttttcggtc tgccggtgac ccaggagata cgtgtcggtc tgatcatcgt
     3481 caccacggcc attgcaaccg tctccgtggt tctcggcctg gaccggggca tcaagcggct
     3541 gtccgagatc aacatcgtcc tggccgtcat gctgatgatc accgtgctgg ccatcggccc
     3601 gaccaccctg ctcctccagg cgttcatgca gaacaccggc gactacctgg ccgacctggt
     3661 gggaaagacg ttcaacctct acgcctacga ccccacggac tggctggggg gatggaccat
     3721 cttctactgg ggctggtgga tcagctgggc gcccttcgtg gggctcttca tcgcgcggat
     3781 ctcccggggc aggaccctgc gggagttcgt gatcggcgtg ctgatcggtc ccgtcgtctt
     3841 caccttcctg tggatgaccg tgttcggcaa cagcgccatc gatctgatca tgaaccaggg
     3901 cgtgacggag ctcggctcgg ccgtgcaggc caacgagtcc atcgccctgt ttcgcttctt
     3961 ggagcagttt cctgccaccg gatttctgtc tgccctggcc atcgtgatgg tcgtggtctt
     4021 tttcgtgacc tcggcggact cgggggccct ggttctgaac atgctgtcat ccaacggctc
     4081 ggatcagaca ccggtgggcc ggcggatctt ctggatgagc gccatagggg tgacggccat
     4141 cgtgctgctg ctggtgggcg ggttgtccgc gctgcagacc gccgtcatcg caagcgccct
     4201 gcccttctcc gtggccgtca tggccggaat ctgggggttc agcaaggcgc tctacgtcga
     4261 tcacgccaaa cgcgtgtccc atgcggcagc gaccgtgccg cccattctcg ccaggcccga
     4321 gatctcatgg cagcatcgac tgacacagct gctgcgccac ccccaggacg ccgaagtgag
     4381 ggccttcatg cgcgagcggg tcatgcccgt gatgcgcgag gtcggcgacg agctcaagcg
     4441 ccacgggctc gatgtggagg tgatcgtgga tggcggcgag ggcgagtcgt tacgcctgga
     4501 gattgcccac ggcgaggaaa tcgacttcgt ctacgaggtg gtgaaccgga agcatccgct
     4561 tccggacgag gccctggtcg gcaccgccat gaaggacatg ccgccatcga acacttatca
     4621 ccgggccgag gtgcacctga gagaaggaag ccaggactac gacatcatgg gctggacgag
     4681 cgagcagatc gctcacgaca tcaccgagca gtacgagaag caccggcact tcctgcacat
     4741 gctgcgctga tcggcggcag cctagtagga aatggactcg gcgagcgtga cccagcgacc
     4801 gccctggacc tgagaaagca cggactccgt ggcgccgcta tgcttctccg gaccgaaaga
     4861 tacgttgtag ccaaacaggt ccgtgtagtc cgtgatgccc tctaacgcgg caaggaggct
     4921 ccctacgctg agatcgggac ccgccttctc cagggcgagc acaagcagat ctgccgcccg
     4981 atagccttcc atggcagcca gacccggttc ctcgtcgtac agcgccacgt aatgatccca
     5041 ccacgcctgc acggcgggcg ttttctcgtc atcggcgtaa atcttcgcca tgtggacgaa
     5101 cgcgtaatag ccttcgcagc tcggcgagtc ggcgatgacc tgcccgtagg ctgcgttgtt
     5161 gccgacccag gcgacgtctt cccagcccat cttgcgcgcg ctgtcgagca cgagaatggt
     5221 gtcgcggtgc acggttccca tcaggacgag gtcgcagccg gcattgcgca gccgcaggat
     5281 ggcggcggtg aactcgctct ccgagggctt gtgcgccgcg gactcgacga gggccatgtt
     5341 catctctgcg agctggtcgt tgaccgcctc gagaatctcg tgcccgtagt cggtgtcgtg
     5401 atagatcgcg cagaccgact gcttgccctg gctgtcgacg aagtaacgaa tggccgctcg
     5461 cacctcgtag tagtagatgc cccgctgggt gaacttcaaa gggtgcagcg gctccaccat
     5521 cgagcgcgcc ccgctgatgg gaaagaggtt cggcacgccc tgctcgaact gctccatcat
     5581 gatggcgttg ttcatcggcg ttccaaccgc gagcaccatg gccagcacct ggtcacggtt
     5641 gatgagcttg ttggcggccc gtatggcgtt gggaacctga tagccggcgt cctccaccac
     5701 gtagcggatg ctgcggccat gcacgccccc ggcggcgttc gcctcgtcga agcgcatccg
     5761 tgcgccgttg gtggcaccga ctccccagat cgccgtgggg ccggagaggt cggtgtgcac
     5821 gccgaacacg acctcgttcg tttgggtcgc ctctccaccg cacgccccaa gcaggagcag
     5881 acttaagatc gccatgaggc cgttgcgata gacgttttcc attcagctct ccagtgtgcc
     5941 cgtgcgggcg tcagtacatg gtgtcgatga gctcgccata cttctcgtgc accaccttgc
     6001 gcttcagctt catcgtcggc gtcagctcgt cgtcttcctc gtcgagcagc tggtcgatga
     6061 gccggaactt cttgatggtc tccacccgcg cgaaccgcgc gttcaccttc tctatctcgg
     6121 cctccacgag atcctgaacc tcgcgtgcgt ggcacaggct cgtatagttg gtgaacggca
     6181 cgtcatggtc ctgagcatac ttcgtcacgt tctcgtgatc gatcatgatc aggcaggtca
     6241 ggtaaggacg acgatctccc accaccaccg cgtctgtgat gtagggcgag aacttcagct
     6301 ggttctcgat ctcgctcggc gtgatgttct tgccgccggc ggtgatgatg atgtccttca
     6361 tccggtcgac gacgtagacg aacccgtcct cgtcgatgcg ccctacgtct ccggtgtgaa
     6421 gccacccctg ccggaacgtc tcggcggtct tttcggggtt ttcccagtac ccgcagatga
     6481 cgcccgggct gcggatcagt atctcgcccg cctccgacaa cgtcacctcg atctcctgcc
     6541 cgggcttgcc gacgctgccg atgcgcacct cgccggggtg gttggcggta gccagacccg
     6601 tgcattcggt ctgcccgtac acctcgaaca tgggtcttcc cagcgcccag taccagtcga
     6661 tcagctccgg cgcgatgggt gctgcgccgg tggagagcca acggcagcgg tcgatgccga
     6721 gcagcacgcg gatgttcttg agcaccaggg gatcgacggc gcggtacaac aggcgcgata
     6781 tccaagacgg ggtttcgcca cgctggcggg catgggccag gggcgttgcg atgcgcatgg
     6841 cgagatcgta ggcccaccgc cccagcacgg tcccctcctt gagcttgatc gtcaccagcg
     6901 agtgcttctt ctcccagacg cgcggcaccg cgaaatgaat ggtaggcgcg atctcctgct
     6961 gatccctgtc cgtggtctcg agctcctcga ccagattgat cacggagcac gactcgatca
     7021 ccgcgaagtt gtagaacatc tgggccgcca catgagccgt gggcaggaac cccagctgct
     7081 cgtcggccga ggtaatgccg tacatgcgct gaaggttccg catcatgaag atcatgttgc
     7141 ggttactgat catcgcgccc ttgggattgc ccgtggtccc ggaggtgtag gtcaggatga
     7201 gcaggtcctc cgcctcggca gcctcgatct cctgctccca cagcccgggg tgctgctcgc
     7261 cgtactcgcg accgagcgcg agcagcgcat cgaagctcat gcactgctcg tcggagaacg
     7321 tgcgcagccc ctccatgtcg aagatgatga tcttctccag cgtcggcgtg ttgccgcgga
     7381 cctcgagcac cttgtcgagc tgctcctcgt cctcggcaaa atagaaacgc gtccggctgt
     7441 cgttgatcag atactcgacc tggttcggcg catcggtggg gtagacgccg ttgacgatgc
     7501 cgcccacgcc gatcacgccg tgatcggcga aaagccactc cttgttgacg tcggtcgcga
     7561 tggagcagac gtcgccccgc ttcaggccca gggctttcaa ccccagaccc accagccggg
     7621 cctgctcgcc ccagtcgccc caggtgtact cgttccagat cccgaagtcc ttctcgcgca
     7681 gggcgatctt gtcgggcagg tccgtggcgc gcttccagaa gagccgggca accgtcgcgc
     7741 agccgtcgat gatctgcggg ctcatcgcca agtcttcctc tgcttccagc ggcgccggcc
     7801 acgcacgccc tcgtctttca tgccgaggta gaactccttg atgtccgtgg cgttcttgag
     7861 ccgctcacag gtgtcttcca tgacgatgcg gccgatctcc atgacgtagc cgtagtcgcc
     7921 gatgtccagg gccatcttcg cgttctgctc gaccagcagc atggtcacgg cctgctcgcg
     7981 attgaggcgg acgatgatct ctgcgatctc cgcgacaagc cggggcgaca gcccgagtga
     8041 cggctcatcg agcagcatca gccgcggccg cagcatgagc gccctgccga tggcgagcat
     8101 ctgctgctgc ccgccggaca ggtactcggc ctgctgcctc gcgagctcct tgagaacggg
     8161 aaaatagccg tacaccagct cgatgtcgtc gccgatctcg gcgtcccggc gggagaacgc
     8221 gcccatgcgc aggttgtcct caacgcagag aaaggggaag acctcgcggc cttccgggac
     8281 gtgcgcgatg ccgcggcgcg ccacccagtc cgggtcacga gcctggatcg gctcgccctg
     8341 gtaggtgatg gtgcccttct gaggatccat ggcgccgctg atggtcttga gcacggtagt
     8401 cttgccggcg ccgttggcac cgagcaggga gacgatcttg ccctcgggaa cctccatgct
     8461 gacgccgcgg atggccatga tggggccgta ataggtctcc acgttcttga gctcgagcat
     8521 cgcgtccacg gtcaatcccc caggtacgcc cgcaggacgt ccgggtgctg ctggacgtcg
     8581 gccggagacc cgatggtcag cagcgccccg tccgccagcg ccatgacccg gtccgaagcg
     8641 cgggagacga gattcatgtc gtgctccacc atgatgatgg tgatgccgag atcgttgttg
     8701 atgtcctcca gccagaagga gaggtcctcg gtctcctccg ggttgagacc ggacgaaggc
     8761 tcgtccagca gcagcagctt cgggcccagg cacagcgccc tggcgatctc caccatcttg
     8821 cgaacgccat agggaagatt gccgatcacc tgatcacggt agctctgcag atccagcaga
     8881 tcgatgacgt cctccacccg cttgcgaaaa gtcagctcct gggccttcac ggacggcaga
     8941 aagagaatgt cggcaagcag gccggaacgt cgatggacgt gacagccgat cagcagattt
     9001 ttcagcaccg tttcctgctc gaacagctcg gtgttctgga acgtgcgcgc gacgccgagc
     9061 tcggcaacgg cgtgggccgg caacctggtg atctctgtgc cgtcgaaggc aagggacccc
     9121 tggtccacgt cgtagattcg gctgatgagg ttgaagatcg tcgttttgcc cgcgccgttg
     9181 gggcctacga tgctgaacac ctcgccgtct tccacggcaa aggaaaggtc gtgcacggcc
     9241 ttgatgccgc cgaagctcac cgcgacgtcc tccgcggcaa aaaggctcac cgcaatcgct
     9301 ccgtgcgcag atacgtcttc tgccgcttgt aggtcgcccg ccgatagagc ggaaactcct
     9361 cgaagaaaag gcggatcttg acccagcggc cgtatatacc cagcggttcg aatatcagga
     9421 agagcacgag aatgaggccg aatatgccgg gctccagccc cggtatctgg gcgatcgccg
     9481 gcggcaccac gtccctgaac agggcgatcc cctggggcag caggccgatg aagatggccc
     9541 cgaacacgac cccgtgcagc gagccgaggc cgcccacgac gaccatcagc agcagctgaa
     9601 tggaggtgag cacggtgaac gagtccggaa agaggtagcc aatccggtgc gcaaacagcg
     9661 cgcccgcaag acccgtgaag ccggccgaca gggcgaaggc cagccccttg atgcggttga
     9721 tgtgaatgcc catgctctgg gccgaggttt cggagtcccg cacggcgacc atggcccggc
     9781 ccgtagggct gcgcagcagg ttcatcgccg ccagcagacc gagcgcaagc accccaagac
     9841 acagatagta gaagttggcc ggagcgccga ggtcgaagcc gagaaactga gccttgggca
     9901 cgggcaggcc acgcaggccg ccggtcaccg tctcccattt ctccagcacc ttctcgacga
     9961 tcacggccag ggcgagggtc gccacggcca ggtaaatgcc cgtcatccgc agggcgggca
    10021 cgccgaccac cgcgccgacg ccggcggtga ccagggtcgc cgcaaccatt gccaggggca
    10081 gaggcacgcc gttgcccagc agccacgaat ggcagtacgc gcccacggcc agaaaggccg
    10141 catggcccag gctgacgagg cccgtgtacc ccaccagcag catcagcccg atgccgg
//
LOCUS       DBNHKV010000077         3296 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_720497, whole genome shotgun sequence.
ACCESSION   DBNHKV010000077 DBNHKV010000000
VERSION     DBNHKV010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_720497"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="AC16PP_02290"
     CDS             <1..945
                     /locus_tag="AC16PP_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012593.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR6480482.1"
                     /translation="GAGVAPASLGTDFQPAEPTSIKDTLVSIIPTNPVSAMAEGRMLQ
                     IILFALLLGLALSRSGQAGRRLAGVFDDLNEALMKLITLLIKLAPIGVFCLMAKLFAT
                     LGWSEITRLLQYFLTVVGALLIHVTLVYPTLLMVLGRVSPIRFFGKMREPLLVAFSTA
                     SSGATLPVTLRTVEERVGVKNEVASFVVPLGATINMDGTAIMQGVATVFIAQLYGIEL
                     GMSQFLIIILTATMASVGTAGVPGVGMIMLVMVLEQVGLPLDGIVFILAVDRLLDMLR
                     TAVNVAGDGMVATVVARSENDLDLDTFEDPDAGRVIGS"
     gene            complement(967..2979)
                     /gene="recG"
                     /locus_tag="AC16PP_02295"
     CDS             complement(967..2979)
                     /gene="recG"
                     /locus_tag="AC16PP_02295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019694287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR6480483.1"
                     /translation="MIAEQRVESLKGVGPSLKAKLERLGIFRVGDLLLHLPIRYQDRS
                     RVVPLRDVHPQQECLVQGQVVDSSLGYGRRRSWLVTIEDGTGYLGLRFFHFSKRQQSG
                     LQPGTYVRCFGEIRFGPRGGGPGLEMAHPEYRAFTSEPPDPDPELTPVYPTTKGLGQN
                     RLRGLIDQALSLEWPSDSGAPYQTLAYLHRPPADAPAADIERAQERVALDELTAYYLV
                     MKQRQRDRAKSRALPLPQSQQLGRELLRRLGFRLTHAQRRVITEVLEDLSSETPMLRL
                     LQGDVGSGKTVVAAFAAIRAAEHGRQTALMAPTEILAEQHYVNFSAWLEPLGIPVVLL
                     TGSQSAKERAARQQALEEGEALVAVGTHALFQDKVNFNALALTIVDEQHRFGVHQRMA
                     LRSKGAAPHQLVMTATPIPRTLTMALYADMDVSVLDELPPGRTPIDTRVISDARKEEV
                     IRSAADAMARGEQVYWVCPLIEDSEQLDAAAAEPTAELLAERLEPPIGLLHGRMTGEQ
                     KAKAMMDFKEGRTRLLVATTVIEVGVDVPNATLMVIENPERMGLAQLHQLRGRVGRGE
                     KASRCLLVYKTPLSAAARQRLSVMRESQDGFHIAEKDLELRGPGELLGARQTGEQQFR
                     VADLARHSHLIAEVLARGERLLQTDPTTARELLNAWAPEDNGHIAV"
     gene            complement(3009..>3296)
                     /locus_tag="AC16PP_02300"
     CDS             complement(3009..>3296)
                     /locus_tag="AC16PP_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017700834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HIR6480484.1"
                     /translation="LVPATMDMVAGDVIEQAHQVFRNLRAVASAAGGSLDDLVKVTVY
                     LTDLNDFQSVNEVMTAYFSEPYPARAAIGVAALPRGAAVEVEGVMAHPGDG"
BASE COUNT          518 a         1077 c         1103 g          598 t
ORIGIN      
        1 ggtgccggcg tcgcgccggc ctccctggga accgacttcc agccggcgga gccgacgagc
       61 atcaaggaca cgctggtctc catcatcccg acgaacccgg ttagcgccat ggcggaaggc
      121 aggatgctgc agatcatcct gttcgctctt ctgctcggcc tggccctgag ccggtcgggg
      181 caggccggcc ggcggctggc cggcgttttc gacgatctga acgaagcgtt gatgaagctg
      241 atcacgctgc tgatcaagct cgcgcccatc ggggtcttct gcctgatggc gaagctgttc
      301 gccaccctgg gctggtcgga gatcacgcgg ctcctgcagt atttcctgac ggtggtgggc
      361 gctttgctca ttcacgtcac cctggtctac ccgacgctgc taatggtgct gggcagggtc
      421 agcccgatcc gtttcttcgg caagatgcgc gagccgctgc tggtggcctt cagcaccgcc
      481 tcgagcgggg cgaccctgcc ggtcaccctg cgcaccgtgg aggagcgggt tggcgtgaaa
      541 aacgaagtgg caagcttcgt cgtgcccctg ggcgcaacca tcaacatgga tggaaccgcc
      601 atcatgcagg gggtggcgac ggtgttcatc gcgcagctgt acggcatcga gctgggaatg
      661 tcgcagttcc tcatcatcat cctcacggcg accatggcct ccgtcggcac ggcgggggtc
      721 cccggcgtcg gcatgatcat gctggtcatg gtgctggagc aggtcgggct gcctctcgac
      781 ggcatcgtct tcatccttgc cgtggaccgg ctgctggaca tgttgaggac ggcggtcaat
      841 gtggccggtg acggcatggt ggcgaccgtg gtggcccggt ccgaaaacga tctggatctc
      901 gacacgttcg aggaccccga tgccggccgt gtgatcggct cataagagct gcccggaagg
      961 gccctcctac acggcgatgt gcccgttgtc ctcgggtgcc caggcattga gaagctcccg
     1021 ggccgttgtc gggtccgtct gcaggaggcg ttcgccgcgt gccagcacct cggcaatcag
     1081 atggctgtgg cgggcgagat cggcaactcg gaactgctgc tcgcccgtct ggcgcgcgcc
     1141 cagcagctcg cccggtccgc gcagctccag gtccttctcg gcgatgtgaa agccgtcctg
     1201 gctctcccgc atgaccgaaa gacgctggcg tgccgccgcg ctcagcggtg tcttgtagac
     1261 cagcaggcat cgcgatgctt tctctccccg cccgaccctg ccgcgcagct gatgcagctg
     1321 tgccaagccc atgcgctccg ggttttcgat gaccatcagc gtggcgttcg gcacgtctac
     1381 gccgacctcg atcaccgtgg tcgcgaccag cagccgcgtg cggccctcct tgaagtccat
     1441 catggccttg gctttctgct cgccggtcat ccgaccatga agcaggccta tcgggggctc
     1501 gaggcgctcg gcgagcagct cggcagtggg ttccgcagcg gcagcgtcga gctgctcgga
     1561 atcctcgatg agggggcata cccagtagac ctgctcaccc cgagccatcg catcggctgc
     1621 gcttcgaatc acttcctcct tgcgggcgtc gctgatcaca cgggtgtcga tgggcgtccg
     1681 ccccggcgga agctcgtcga gaacggagac gtccatgtcc gcgtacaggg ccatggtcaa
     1741 cgtgcgcgga atcggcgtgg cggtcatgac cagctgatgg ggcgcggccc ccttgctgcg
     1801 cagcgccatg cgctggtgca cgccgaaccg atgctgctcg tcgacgatgg tcagcgccag
     1861 ggcgttgaag ttcaccttgt cctggaacag ggcatgggtg cccaccgcca ccagcgcttc
     1921 gccctcctct agcgcctgct gccttgcggc acgttctttc gccgactggg aacccgtgag
     1981 caacacgacg gggatcccga gcggttcgag ccaggcggag aagttcacgt agtgctgctc
     2041 cgcaaggatt tcggtcggcg ccatcaatgc cgtctggcgg ccgtgctcgg ctgccctaat
     2101 ggctgcgaaa gcggcgacca cggtcttgcc ggagccgacg tcgccttgca gaagccgcag
     2161 catgggggtt tcgctggaca ggtcctccag cacctccgta atcacccttc gttgggcatg
     2221 ggtgagcctg aatccgagcc tgcgcaagag ctcccggccg agctgctggg attggggcag
     2281 cggcagcgcg cgggatttgg cgcgatcgcg ctggcgctgc ttcatgacca ggtagtaggc
     2341 ggtgagctcg tccagggcga ccctctcctg ggctcgttcg atgtcggcgg cgggcgcgtc
     2401 cgccggcggc cgatgcagat aggcgagggt ctggtatggc gcgccggagt cgctgggcca
     2461 ttccaaggaa agggcctgat cgatcagccc gcgcaggcgg ttctgcccta gacccttggt
     2521 cgtggggtag acgggcgtta gctccggatc tggatccggg ggctcggagg taaaggcgcg
     2581 gtactccggg tgcgccatct ccagcccggg accgccgccc cgggggccga aacggatctc
     2641 gccgaagcaa cgcacgtacg tccccggctg cagcccggac tgttgacgct ttgagaagtg
     2701 aaagaatctc aggcccaggt agccggtgcc gtcctcgatg gtcaccagcc agctgcgacg
     2761 gcgaccgtag ccgagggatg aatccaccac ctgcccctgc accaggcact cctgttgtgg
     2821 gtggacgtcg cgaaggggga ccacgcggct gcgatcctgg tagcgaatgg gcaggtgcag
     2881 cagcaggtcg ccgacccgga aaatgcccag gcgctcgagc ttcgccttca agctcggtcc
     2941 gacacctttc agggattcca cgcgctgttc agcgatcacg gcgcctgccg gctcagggct
     3001 ttgacgaatc agccgtcacc ggggtgagcc atgacgcctt ccacctccac ggccgcgcca
     3061 cgtggcagcg cggccacgcc gatcgcggcg cgcgcgggat acggctcgct gaaataggct
     3121 gtcattacct cattgaccga ctgaaagtcg ttgagatccg tgaggtagac ggtgaccttc
     3181 accagatcgt ccagggaccc gcctgcagcg ctggcaacag cgcgcagatt cctgaatacc
     3241 tgatgcgcct gctcgatcac gtcgcccgca accatgtcca tggtcgctgg aaccag
//
LOCUS       DBNHKV010000078         4371 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_650196, whole genome shotgun sequence.
ACCESSION   DBNHKV010000078 DBNHKV010000000
VERSION     DBNHKV010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_650196"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1647
                     /locus_tag="AC16PP_02305"
     CDS             157..1647
                     /locus_tag="AC16PP_02305"
                     /inference="COORDINATES: protein motif:HMM:NF014165.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480485.1"
                     /translation="MSAYSELEAKAREVARLREVAAITHWDEACMMPRGGGAGRSEAL
                     ASLATLIHERVADPEIGELLNRARGEGFELDDWQSANVTNMEREFVEATAIPSDLVRA
                     SRLAQMKCEQAWREARARNDWETVKPLLAEVIGLARQQADALADAMHLSRYDALLEVY
                     EPELRIADIEPVFEDLKAFLPGALETALDRQGEVAPLEGPFTVEEQRQLSLEVMGLLG
                     FDFDRGRLDVSHHPFCGGVPDDTRITTRYSESSFIEALMATCHETGHALYQQGLPRDW
                     RQQPVGDSLGAAVHESQSLLMELQVCRSREFVGFLAPMIRRHFDRSETEPAWSTDNLY
                     GHVTRVARGYIRVDADEITYPLHVILRFELERQLLDGTLAVADLPEAWNDAMQRYLGL
                     STEGNNADGCMQDVHWYAGLFGYFPTYTLGALTAAQWFATARKVMPDLASDIAAGNLE
                     PLVAWLRENIHGRGRQTTMQPLLEEVTGSTLDAGFYKRHIQARYLG"
     gene            complement(1693..2772)
                     /locus_tag="AC16PP_02310"
     CDS             complement(1693..2772)
                     /locus_tag="AC16PP_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015648740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HIR6480486.1"
                     /translation="MHGLQLLVNRNDLEDTRWQQAPVPAPDELAPGEVLLRIDEFALT
                     ANNITYAVAGEMMRYWNFFPAPDGWGRVPVWGFATVVASNHAEVATGERLYGYLPMGS
                     HLIIEAGRVRANQLTDVSAHRVDLPPVYNQFQRVDAASEPAADAAQMLYRPLFMTSFV
                     LDHFLHANGFFGAQSVILTSASSKTALGLALLLARRGDVRVIGLTGRANVGFVEGLGC
                     YDEVVAYEDLATLDAAREVVSVDMAGNGEVLAALHNHFADNLKYSCLVGATHWDHRAG
                     AREMAGPEPELFFAPSHIQDTDWGPGGLEARFADAWDMLVERMSGLVTVVRADDPAEV
                     EAVYQRLLAGEVGPDVGYVLGVDVG"
     gene            complement(2788..3657)
                     /locus_tag="AC16PP_02315"
     CDS             complement(2788..3657)
                     /locus_tag="AC16PP_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6480487.1"
                     /translation="MELDGFETREIELDGVRMHVRIGGAGPPLLLLHGYPQTHLMWHR
                     IAPRLARNFFVVAPDLRGYGASGKPPAGEEYRAYSKRVMAAEMVALMAGLGHERFAVA
                     GHDRGGRVAYRMALDHPEVVSRLVILDIIPTLDQFEALGWRGGLFGYHWYFLAQPPPL
                     PETLIAHEPELYLRTAMSSWAGDFSALDAAFPAYLAAFTPETIRASCDDYRAGAYVDT
                     EIDRADREAGRRIGCPVLALWGEINGPRPGVLEAWQRWADDVQGVGLPCGHFLPEEAP
                     EAVLAAMLEFLKA"
     gene            4065..>4371
                     /locus_tag="AC16PP_02320"
     CDS             4065..>4371
                     /locus_tag="AC16PP_02320"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6480488.1"
                     /translation="MTAQKREESMQDVAISMSAMDANAIEKSFSRTIDEITGMSPNLV
                     INPILGNGTVGVSIRGMQHAEVEKSFDPAVAIYQDGIYLSSTTGALLNVWDAERVEVL
                     "
BASE COUNT          823 a         1473 c         1377 g          698 t
ORIGIN      
        1 tggtgtcgtg tccaaaagcc atgccgctga tcccccaaaa aatacccgct caatcctatc
       61 catccggcca tcggggcgcc acggcccaac gctcccaaaa cgacgccgaa ggtctttata
      121 ctgcgccgct ccaaattcaa tcccgccagg aagaccatgt ccgcctactc ggagctcgaa
      181 gccaaagccc gcgaagttgc ccgcctgcgc gaggtggcag ccattaccca ctgggacgag
      241 gcctgcatga tgcccagggg cggcggcgcg ggccgctccg aagcgctggc gtccctggcc
      301 acactgatcc atgagcgcgt ggcggatccg gaaatcggcg agctgttgaa tcgcgcgcgt
      361 ggggaggggt tcgagctcga tgactggcaa tcggccaacg tgaccaacat ggagcgggag
      421 ttcgtggaag ccacggcaat cccttcggac ctggtgcggg cgagccggct ggcgcagatg
      481 aaatgcgagc aggcctggcg cgaggcccgg gcgcggaacg actgggagac ggtgaagccc
      541 ctcctcgcgg aggtgatcgg tctcgcccgt cagcaggcgg atgccctggc cgatgccatg
      601 catctgtccc gctacgatgc gcttttagag gtctacgagc cggagctgcg catcgccgac
      661 atcgagcccg tcttcgagga tctcaaggcg tttctccccg gcgcgctgga gacggccctg
      721 gatcgccagg gcgaggtggc gcccctcgag gggcctttca cggtggagga gcagcgtcag
      781 ctgtccttgg aggtcatggg cctgctcggc ttcgacttcg atcggggcag gctagacgtc
      841 agccatcacc cgttctgcgg cggcgtgccg gacgataccc gcatcaccac ccgttactct
      901 gagtcgagct tcatcgaggc gctcatggcg acctgccacg aaaccgggca cgcgctctac
      961 cagcagggtc tgcccaggga ctggcggcag cagccggtcg gcgactcact gggtgcggcc
     1021 gtccacgaaa gccagtcgct gctcatggag ctgcaggtct gccgcagccg cgagttcgtc
     1081 ggctttctgg cgcccatgat ccgccggcac ttcgaccgca gcgagaccga gccggcgtgg
     1141 tcgacggaca acctgtacgg ccacgtcacc cgcgtggcgc gcggctacat ccgcgtggac
     1201 gctgacgaga tcacctaccc gctccacgtc atcctgcgtt tcgagctgga gcgccagctg
     1261 ctggacggca ccctcgccgt ggccgacctg cccgaggcat ggaacgacgc catgcagcgc
     1321 tatctggggc tgtccaccga gggcaacaac gcagacggtt gcatgcagga cgtgcactgg
     1381 tacgcgggcc ttttcggtta cttcccgacc tataccctgg gcgcgctcac cgccgcgcaa
     1441 tggttcgcca ccgcccgaaa ggtcatgccg gatctcgcct cggacatcgc cgccggaaac
     1501 ctcgagccac ttgtggcctg gctgcgggag aacatccacg gccgcggtcg gcagacgacc
     1561 atgcagccgc tcctggagga agtcaccggc tcgacgctgg acgccgggtt ctacaaacgg
     1621 cacattcagg cgcgttatct cggctgaggc cgcctcgcca cgatcggatc gccccaaaag
     1681 gggcggcgcg attcaaccca cgtcgacacc cagcacgtag cccacgtctg gtcccacctc
     1741 tccagcaagc agccgctggt agacggcctc gacctctgcc gggtcatcgg cccgaaccac
     1801 ggtgacgagc cccgacatac gctccacaag catgtcccag gcatcggcga agcgggcttc
     1861 gagcccgccg gggccccagt ccgtgtcctg aatgtggctc ggcgcgaaga acagctccgg
     1921 ttcggggccc gccatctccc tcgcgccagc gcggtgatcc cagtgggtgg cccccaccag
     1981 gcagctgtac ttcaagttgt ccgcgaagtg gttgtgcagg gcggccagca cctcaccgtt
     2041 accggccatg tcgacgctca ccacttcccg tgcagcatcc agagtggcca agtcttcata
     2101 ggccactacc tcgtcgtagc agccgagccc ttccacgaac ccgacgttgg cccggccggt
     2161 gagaccgatc acgcgaacgt ccccgcgccg cgcaaggagc agcgcaagcc cgagagccgt
     2221 cttgctggac gcgctggtca gaatcaccga ctgcgcgccg aagaagccgt ttgcatggag
     2281 aaaatggtcc agcacgaaag aggtcatgaa caacggccga tagagcatct gggccgcgtc
     2341 agcggccggc tcggaagcag catcgacgcg ctggaactgg ttgtagaccg ggggcaggtc
     2401 gacgcgatgg gcgctgacgt cggtaagctg gttggcccgc acccggccgg cttcgatgat
     2461 cagatggctg cccatgggca ggtagccgta gaggcgctcg cccgtagcca cctcagcgtg
     2521 attcgacgcg accacggttg caaatcccca gaccggcacg cggccccagc cgtcgggcgc
     2581 tggaaagaag ttccagtagc gcatcatctc cccagccacc gcataggtaa tgttgttggc
     2641 ggtgagggca aactcgtcga tgcgcagcag cacctcgccc ggggccagct cgtcgggagc
     2701 cgggaccggc gcctgctgcc agcgggtgtc ctcgagatcg ttccgattaa ccagcagctg
     2761 aagaccgtgc attcatcctc tccctattca ggctttgaga aattctagca tcgcggccag
     2821 aaccgcctcg ggtgcctcct cgggcaggaa gtggccgcag ggcagcccga cgccttggac
     2881 gtcgtccgcc cagcgctgcc aggcctcgag cactccaggc cgcggcccgt tgatctcgcc
     2941 ccacagtgcc agcaccgggc agccgatccg acggccagcc tcacgatcag cccgatcgat
     3001 ttcggtgtcg acgtaggctc cggcccggta atcgtcgcag ctggcgcgga tcgtctccgg
     3061 ggtgaaggcc gcgaggtacg caggaaaggc cgcatcgagg gcagagaagt cgccagccca
     3121 gctgctcatg gccgtgcgca ggtagagctc cggctcgtgg gcgatgaggg tctccggcag
     3181 gggcggtggc tgagcgagga agtaccagtg gtagccgaaa agaccgccgc gccacccgag
     3241 ggcctcgaac tgatccaggg tcggaatgat gtccaggatg acaagccggc tcaccacttc
     3301 cggatgatcc agcgccatgc ggtaggccac gcgaccgccg cggtcgtgcc cggccacggc
     3361 gaagcgctcg tgaccaaggc ccgccatcag cgccaccatc tccgccgcca tcacccgctt
     3421 ggaataggcg cggtattctt ccccggccgg tggcttgccg ctggcaccgt agccgcgcag
     3481 gtcgggcgcc accacgaaaa aattccgcgc gagtcggggg gcaatccggt gccacatgag
     3541 atgagtctga gggtagccgt gcagcagcag gaggggcggt ccagcgcccc cgatccgaac
     3601 gtgcatgcgc acgccatcca gttcgatctc gcgggtctcg aagccgtcca gctccattgt
     3661 tatcccccgg cgttctgcct cgacggcccc gacctactcc cgggcgagcc gggtccaagt
     3721 gccccgatcg ttatccaata cccggccctt caggtcgtta acttcgtacc cgtaacgtgc
     3781 ccgctcggtg cagggagggg acatttcaga ccggaatcac atcaacccgc accaacacgc
     3841 tccaggcgtc cgagctctcg aggcgactgg actccgtttg tacatcaaag tagagtcccc
     3901 gcccgaatcg cttccacagc agaagcggcc acgagataag actatcgagg ggaaacatgc
     3961 caaagcaaat gcacataacg ttcatcctcc tggcgatctc gtcaacgttc gccgaaaacg
     4021 ttcgtgccga gagaacgacc accacgcaga tcgaagagat cgtcgtgact gcgcagaagc
     4081 gcgaggaaag catgcaggac gttgccatct caatgtccgc catggatgcc aatgcgatcg
     4141 agaaatcatt ctcccgaacg atcgacgaga ttaccgggat gtcgcccaac ctggtgatca
     4201 acccaatcct ggggaacgga acggttggcg tgtcgattcg cgggatgcag cacgctgagg
     4261 ttgaaaagag tttcgatccg gccgtcgcca tctatcagga cggtatctat ctgtcatcga
     4321 ccaccggcgc cttgctgaac gtctgggacg ccgagagagt cgaagtgctc a
//
LOCUS       DBNHKV010000079         9718 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_365800, whole genome shotgun sequence.
ACCESSION   DBNHKV010000079 DBNHKV010000000
VERSION     DBNHKV010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9718)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9718)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9718
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_365800"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /gene="purH"
                     /locus_tag="AC16PP_02325"
     CDS             complement(<1..543)
                     /gene="purH"
                     /locus_tag="AC16PP_02325"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786862.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR6480489.1"
                     /translation="MSNADARSVARALLSVSDKTGLEEFARGLSALGVEILSTGGTYR
                     ALSEAGIRVTEVSAHTGFPEMMDGRVKTLHPKIHGGILGRRDVDGATMTEHDIEPIDL
                     VVVNLYPFEATIERADCTDELAIENIDIGGPAMVRSAAKNHRDVLVVVDPGDYGHLLE
                     TLQRDGATTLESRRALAAKAF"
     gene            complement(540..638)
                     /locus_tag="AC16PP_02330"
     CDS             complement(540..638)
                     /locus_tag="AC16PP_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6480490.1"
                     /translation="MARIGGNKSRAATMLGMNRGTLRKKLKGYGII"
     gene            complement(638..1627)
                     /gene="dusB"
                     /locus_tag="AC16PP_02335"
     CDS             complement(638..1627)
                     /gene="dusB"
                     /locus_tag="AC16PP_02335"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR6480491.1"
                     /translation="MTVTAIGPFKLRNDVLLAPMAGITDAPFRALAWRFGAGYVVGEM
                     VGSREALWHTPKSRLRRCRVDGVEPFAVQIAGADPRDVAESAARHVEEGAQVIDINFG
                     CPVKKVCNKAAGSALLKEPDLVVAIVRAAVAAVPVPISVKMRTGWSPDSRNAVELAQR
                     MEDEGVAAIAVHGRTRACRFNGEAEHGTVAAIKSRVSIPVFANGDIDSPASARRILEE
                     TGVDGVMIGRAALGRPWLLGEVADGVPRVASLGEKLAVVAEHVAALHEFYGEPGTRIA
                     RKHVQWYLQHLSQLGPREVRSRLGKAFNRLEQAQAQLDFLAELPASAIGRRAA"
     gene            complement(1658..2575)
                     /locus_tag="AC16PP_02340"
     CDS             complement(1658..2575)
                     /locus_tag="AC16PP_02340"
                     /inference="COORDINATES: protein motif:HMM:NF023334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HIR6480492.1"
                     /translation="MIEQKLESMITECPHCQTRFRVTDVQLEAAAGRVRCGSCLNVFQ
                     GARHMVPARVPEPAPRRTATVRHLYGGHEAQPPSAEPDESSQVTGARDAASPPPPPEA
                     DHAARAAEPESADTPSAAGQGAALEVPRSLKKLSAAEAQGLALYAQPPRRRWWAPLAG
                     AAAVLLVATQVLWLQFDTWARDPQVRPLYAMVCGVVGCELPVMRDVESLLAKNLVVRR
                     DPERTDALLVNAIIVNQAAFAQPFPVLELSFTALNGSPVKEERFEPGTYLAGEMAGAT
                     DMPPRTPVHIELTVAHPGDMAVNYSLSFR"
     gene            complement(2624..3340)
                     /locus_tag="AC16PP_02345"
     CDS             complement(2624..3340)
                     /locus_tag="AC16PP_02345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR6480493.1"
                     /translation="MWRRSRLSALFSADADVEALREFLAAHGDVDEGFVEDQDWVDAW
                     RSSAVAECFGGRLWVVPRDSEARNGTVLRLDPGLAFGTGGHPTTRLCLGWLASHDLAG
                     QRLVDYGCGSGILGIAAALLGAAQVLAVDHDPQALLATEENARANAVDARRLRAVGPD
                     EVPGRGDYDVLVANILANPLLELASRFTDLIRPGGMIVLSGLLARQASEVVAGYPSFR
                     FADPQPDGDWVLLSGRKRDR"
     gene            complement(3491..3889)
                     /locus_tag="AC16PP_02350"
     CDS             complement(3491..3889)
                     /locus_tag="AC16PP_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010795424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR6480494.1"
                     /translation="MDLRKIKKLIELVEESGIAELEVRDGEEAIRIARPVAGTVPAPV
                     AQVRAAQPGAVAGTITAPMAGTFYAASEPGAEPYLKVGQQVEVGDVVCIIESMKMMNE
                     IVADQSGTCVSVAVGNGQAVASGQVLFNIA"
     gene            complement(3902..4333)
                     /gene="aroQ"
                     /locus_tag="AC16PP_02355"
     CDS             complement(3902..4333)
                     /gene="aroQ"
                     /locus_tag="AC16PP_02355"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR6480495.1"
                     /translation="MARFLVLHGPNLNLLGTREPEIYGTDTLEDINSRLEALAREAGN
                     EATCLQSNAEHELVDAVHGAKTQGVEGIVINPGAFTHTSVALRDALLGVGIPFIEVHL
                     SNVHAREEFRRQSYLSDVARGVITGLGAKGYEFALLAAMAR"
     gene            complement(4421..6331)
                     /gene="dsbD"
                     /locus_tag="AC16PP_02360"
     CDS             complement(4421..6331)
                     /gene="dsbD"
                     /locus_tag="AC16PP_02360"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     [NAD(P)H] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HIR6480496.1"
                     /translation="MRRFWPGLGAAAIGFTLLLAAPGAAASALDDISTGAAGWLSEQP
                     TFLPVDEAFAMSAEVGPDGAILVRWDIADGYYLYRHRFTFEAREPAGGGAIAVLGAPE
                     IPPGKAKVDEYFGEVEVYYGRATARLPVQGGATVEVGIGYQGCADLGLCYPPETRWVT
                     LTMPGAPGAISGGDAGSAPGSPAATVQTEERALAGLLAEGSLLTALLLFFVAGVGLTF
                     TPCVLPMVPILSSIIVGDSEKLTRSRGITLSLAYVLGMAFTYAIIGTLMGMFGASLNM
                     QAALQSPPVLVGFALVFAALSLSMFGLYELRLPASWQSAIDAVGSRTGGGKHLSVAVM
                     GSLSSLVVSPCVSAPLAGALIYLSATGDAVLGGGALLALGLGMGVPLLLIGASGGHML
                     PRAGEWMNAVKILFGVLLLAVAVWLLERVVPGPVNLALWAALAIGAGGYLGLRAWSPR
                     GMVSGAVRAAGLLGVVYGALLLVGAAGGGNDPLRPLAGLTAWDPAHQPGEALEWQPVD
                     DVAGLRAAVLAASREGKPVMLDLYADWCISCKVLERSVFPKPEVAARLSRFNLLRADV
                     TANDDHHKALLDELGVFGPPSMVFFAEDGRKLAEVTVQGEIGADALAAHLGAVLERVG
                     SNNFSEIAVNFR"
     gene            6545..7165
                     /locus_tag="AC16PP_02365"
     CDS             6545..7165
                     /locus_tag="AC16PP_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480497.1"
                     /translation="MPEGARADPLTEPAPAPAPHAAEIDALLLQANDAMERGQYINPA
                     RGSALGLYDRVLALNPGNAEARRGLELIAQHYLDLASAAVRRRQLTRARSMVDWARIV
                     DAEHPGIAAAEAQIRLLAEARRVRLPVAGSQLRDRSDALARSLAELGSRARNGNCLAI
                     IRSPSDASGRWIYQQMSRAPGTARIRADIEIGSPPMVELLCFENDA"
     gene            7165..7623
                     /locus_tag="AC16PP_02370"
     CDS             7165..7623
                     /locus_tag="AC16PP_02370"
                     /inference="COORDINATES: protein motif:HMM:NF016221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HIR6480498.1"
                     /translation="MPDAAILAAALAAALAAAPVAIPASSAPALAGSELVVTDARVRA
                     LLPGQRHTVAYFAIENRGPASVTLVSAESAAAGAMEIHESRRDGDMYRMRRRAEVEIP
                     ARTTVRFDPGGLHLMLFDVQALGEQMEIELRTSDGRRLKAIFNRIAAGAE"
     gene            7620..8666
                     /locus_tag="AC16PP_02375"
     CDS             7620..8666
                     /locus_tag="AC16PP_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009041985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HIR6480499.1"
                     /translation="MKINWRFWASSNDADVTDGRLATAGRIGLGAVAAYLLVCVLLGW
                     WWDKEPDVFAVRENASSRAELGNEPLVTGYITTATLIRLGETLLDKRGGYLSNDVFPP
                     GVWMDNIPNWEFGVLTHIRDATRTYRNELSRSQSQSAEDPDIVRAEGKFFIDNASWFI
                     PQAESEYRDGIKALSSYLQRLADPTQPDAQFYARADNLQQWLQIVEARLGSLSQRLSA
                     SVGKRQLDVSLAGDAAASQATESPREQEVKTPWLEIDDVFYEARGQTWALLHLLRAME
                     QDFGDVLDKKNARVSLQQIIRELEPTQDTVWSPIILNGDGLGLLANHSLVMASYVSRA
                     NAAIIDLRALLSQG"
     gene            8849..>9718
                     /locus_tag="AC16PP_02380"
     CDS             8849..>9718
                     /locus_tag="AC16PP_02380"
                     /inference="COORDINATES: protein motif:HMM:NF015019.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HIR6480500.1"
                     /translation="MNNIALIPPIFGIVGLIVAFFIFSLVKRYDEGTEEVKRIGDAIH
                     EGAMVFMRREYTMLAGFAAALIVILYIALGTETTIAFIVGALASATAGWIGMYTATKA
                     NVRTTTAAHTAGAAQALSVAFFGGSIMGLTVASMGLLGLGILYYVFGGDPETAHHIHG
                     FGMGASTVALFSRVGGGIYTKSADVGADLVGKVEAGIPEDDPRNPGVIADNVGDNVGD
                     VAGMGSDIFESYCGSMIATIAIASTLALAALTDLAPGMDEGSARAALMGLPLILASTG
                     LICSLVGIALVRLM"
BASE COUNT         1704 a         3416 c         3101 g         1497 t
ORIGIN      
        1 gaaggccttc gcggccagcg cgcgccggct ctccaacgtg gtcgcgccgt cgcgctgcag
       61 ggtctcgagc agatggccgt aatccccggg atctaccacc acgagaacgt cgcgatggtt
      121 cttagctgcc gagcggacca tcgcgggccc gccgatgtcg atgttctcga tggccagctc
      181 gtcggtgcag tcggcgcgct cgatggtggc ctcgaacggg tagaggttga cgaccacgag
      241 atcgatgggc tcgatgtcgt gctcggtcat ggtcgcgcca tccacgtcgc gccggccgag
      301 gatgccgccg tgtatcttcg ggtgcagcgt cttgacgcgg ccatccatca tttccgggaa
      361 accggtgtgg gcggagacct ccgtgaccct gatgcccgct tccgagagcg cccggtaggt
      421 accccccgtt gagaggatct ccacgcccag agccgagagc ccgcgggcga actcctcgag
      481 ccccgtcttg tccgacacac tcagcagggc gcgggccaca ctccgcgcgt cagcgttgct
      541 catatgattc cgtaaccctt gagtttcttg cgcagcgtgc cccgattcat accgagcatg
      601 gtcgccgcgc gacttttatt gccgccgatg cgcgccatca cgccgctctc cgaccgatgg
      661 cagaggccgg cagttcagcc aggaaatcca gctgggcctg cgcttgctcc agtcgattga
      721 acgcctttcc gagacgggag cggacctccc tcggccccag ctggctcagg tgctggaggt
      781 accactgaac gtgctttcgc gcaatgcggg tgccgggctc accgtagaac tcgtgcaggg
      841 cggcgacatg ctccgccacg acggcgagct tctcacccag cgaagccacc ctgggcaccc
      901 cgtccgctac ctcgccgagc aaccatggtc tgcccagggc agcacggccg atcatgacgc
      961 cgtcgacgcc ggtctcctcg agtatccggc gcgccgacgc aggggagtct atgtcgccgt
     1021 tggcaaatac cgggatggac accctggact tgatggcggc cacggtgccg tgctccgcct
     1081 cgccgttgaa gcggcaggcg cgcgtgcggc cgtgcacggc gatggctgcg acgccctcgt
     1141 cctccatccg ctgcgcgagc tccacggcgt tacgggaatc cggcgaccaa cccgtgcgca
     1201 tcttcaccga gatcggaacc ggcacggcgg caaccgcagc gcgaacgatg gcaacaacca
     1261 gatccggctc cttgagcaga gccgatcctg cagccttgtt gcagaccttc ttgaccggac
     1321 agccgaagtt gatgtcgatc acctgggcac cttcttcgac gtgccgcgcg gcactctcgg
     1381 caacgtcccg ggggtcggcg cccgcgatct ggaccgcgaa gggctccacg ccgtccaccc
     1441 ggcagcggcg caggcgggat ttgggcgtgt gccagagcgc ttcacgggag ccgaccatct
     1501 cgccgaccac atagccggcc ccgaatcgcc aggccagcgc gcgaaagggc gcgtcagtga
     1561 tccccgccat gggcgccagc agtacgtcgt tgcgcagctt gaatggcccg atggcggtca
     1621 cggtcacccc ttgaagaaaa ggggcgacat cataacgtca ccggaacgaa agagaatagt
     1681 taacggccat gtcgccgggg tgcgcgacgg tcagctcgat gtgaacgggc gtccgcgggg
     1741 gcatgtcggt ggccccggcc atctcccctg ccagataagt tcccggctcg aatcgctcct
     1801 ccttgaccgg cgagccgttg agcgcggtaa agctcagctc gagcacggga aagggctgcg
     1861 cgaaggcggc ctggttgacg atgatggcgt tgacgagcag ggcatcggtt cgctccgggt
     1921 cgcgccgcac caccaggttc ttggcgagca ggctctcgac gtcgcgcatc accggcagct
     1981 cgcagcccac gacgccgcat accatggcat acagcggcct cacctggggg tcgcgcgccc
     2041 aggtgtcgaa ctgaagccag agaacctggg tcgccaccag cagcaccgcg gccgccccgg
     2101 ccagcggcgc ccaccagcgt ctgcgcggcg gctgcgcata cagggctagc ccctgggcct
     2161 ccgccgcgga gagcttcttg agggatctgg ggacctccag ggcagcgcct tgcccggctg
     2221 cgctaggagt atcggcgctt tcgggctctg ccgcccgcgc tgcatggtcg gcttcggggg
     2281 gcgggggcgg gctcgccgca tcccgggcgc ccgtgacttg cgaggattcg tccggctccg
     2341 ccgaaggcgg ctgcgcctcg tggcctccgt agaggtggcg cacggtagcg gttcgacgcg
     2401 gggccggctc cgggacgcgg gcaggcacca tgtggcgcgc gccctggaag acgttgaggc
     2461 aggacccgca acgcactctc cctgcagccg cctcgagctg cacgtccgtc acccggaatc
     2521 gagtctggca gtgcgggcac tccgtgatca tcgattccag cttctgctca atcatgactt
     2581 tagacttcgt ccgtccctcg accctgcctg cggcttcgat cagtcaccgg tcacgcttgc
     2641 gccctgagag caacacccag tcgccgtcag gctgggggtc ggcgaagcgg aatgaaggat
     2701 accctgctac gacctcgctt gcctgcctgg caagcagccc ggagagcacg atcatgccac
     2761 ccggtcggat aagatccgtg aagcggctcg caagctccag cagcggattg gcaagaatat
     2821 tagccaccag cacgtcgtag tcaccgcgcc cgggtacctc gtccggtccg accgccctca
     2881 gccgccgggc atccacggca ttcgcccgag cgttttcctc ggttgccagc aacgcctggg
     2941 gatcgtgatc caccgcgagc acctgcgccg cgccgagcag cgccgcggca atgcccagga
     3001 tgccggaacc gcatccgtaa tccaccagcc gctggccggc aaggtcgtga gacgcaagcc
     3061 atcccagaca cagccgcgtg gtcggatgtc cccccgtgcc gaacgccagc cccggatcca
     3121 gacgcagcac ggttccgttg cgcgcctcag aatcccgcgg gaccacccac aaacggccgc
     3181 cgaaacattc agccaccgcg ctcgagcgcc aggcatccac ccaatcctga tcctcgacga
     3241 agccctcgtc gacgtcgcca tgggccgcca ggaactcacg aagcgcctcc acgtcggcat
     3301 ccgcggagaa cagggccgac aggcggctac gacgccacag aggggtttca ccggggcagg
     3361 ctcgaggagc agctcatcgg acaggggcgc caggctcacg gacaccgcgc ccagctccag
     3421 caggcgatcg ctcagggcat cggcggcgtc gatctcgaca tcgagctcga actttagcca
     3481 gctcaccggg tcaggcgatg ttgaacagca cctggcccga tgccacggcc tgtccgttgc
     3541 cgaccgcgac cgacacgcag gtaccggact ggtcggcaac gatctcgttc atcatcttca
     3601 tgctctcgat gatgcagacc acgtctccga cctcgacctg ctggcccact tttagataag
     3661 gctcagcgcc gggttcgctg gcggcataga aggtcccggc catcggcgcc gtgatggtcc
     3721 ccgcaacggc cccgggctga gcggcccgca cctgggcaac cggcgccggc acggtgcccg
     3781 cgaccggccg ggcgatgcgg atggcctctt ccccgtcgcg cacctcgagc tccgcgatgc
     3841 ccgactcctc caccagctct atgagcttct tgatcttcct gagatccacg atcagcctct
     3901 cctatcgcgc catggcagcc aggagggcga actcgtatcc cttcgccccg agcccggtga
     3961 tcacgccgcg cgcaacgtcc gacaggtagg attgacgtcg aaactcctct cgggcatgca
     4021 cgttggaaag gtgcacctcg atgaagggaa tgcccacccc gagcagggcg tcccgcaagg
     4081 ccacgctggt atgggtgaag gcgccgggat tgatgacgat accctccacg ccctgggtct
     4141 tggccccgtg cacggcgtca acgagctcgt gctcggcgtt gctctgcagg cacgtggcct
     4201 cgttgcccgc ctcccgggcc agggcctcca gcctcgaatt gatgtcctcg agggtgtcgg
     4261 tgccgtagat ctcgggttcc cgggtgccaa gcaggttcag gttggggcca tgaagcacga
     4321 ggaatcgggc catcgcagga ctccagacgt cacagggcgc cgagtttgca gaaacccacg
     4381 cggaaagaca atacggataa aatgtgaatt tggacgttaa ttaacggaag ttaaccgcta
     4441 tttcagagaa gttatttgat ccaacccgtt ccaggaccgc ccccagatgg gccgccagcg
     4501 cgtccgcgcc gatttcgccc tgcacggtaa cttcggctaa tttgcgacca tcttcagcga
     4561 aaaagaccat gctgggaggc ccgaagacgc caagctcgtc caggagcgct ttgtggtgat
     4621 cgtcattcgc cgtcacgtcc gcccgcagca ggttgaaccg cgacaacctg gctgccacct
     4681 ccggcttcgg gaaaacgctg cgctccagca ctttgcagga gatgcaccag tcggcgtaga
     4741 gatcgagcat caccggcttg ccctcgcggc tggcagccag cacggcggcg cgcagccccg
     4801 ccacgtcatc cacgggctgc cactcgagcg cctcgccagg ctgatgggcc ggatcccatg
     4861 cggtgaggcc cgccaggggc ctgagcgggt cgttgccgcc gccagccgcc ccgacgagca
     4921 gcaaagcgcc gtagacgacg cccagcagcc ccgcggccct gacggcgccc gacaccatgc
     4981 cccgggggct ccaggcacgc aggcccaggt agccgccggc gccaatggcc agcgccgccc
     5041 acagcgcgag gttcaccggg cccggcacca ctcgctccag cagccatacg gccacggcca
     5101 gcagcagcac gccgaacagg attttcacag cgttcatcca ctcgccggca cgaggcagca
     5161 tgtgaccccc gctcgcgccg atcagaagca gcggaacccc catgccgagc cccagggcca
     5221 ggagggcgcc gcctccgagc accgcatctc cggtcgcgct caggtaaatg agggcaccgg
     5281 ccagcggggc cgatacgcag ggcgagacca ccaggctgga cagagagccc atgacggcca
     5341 cgctgaggtg tttgccgccg cccgtccgcg atcccacggc gtcgatggcc gactgccagc
     5401 ttgccgggag ccgcagctcg tagaggccga acatcgacag agacaacgcg gcgaaaacca
     5461 gggcgaaccc caccagcacg gggggcgact gcagcgcggc ctgcatgttc aggctggcac
     5521 cgaacattcc catcagagtg ccgatgatcg cgtaggtgaa ggccatcccg agcacgtatg
     5581 ccagcgagag cgtgatgcca cggcttcgcg tgagcttctc ggaatcgccg acgatgatgc
     5641 tggaaagaat cggcaccatg ggaaggacgc agggagtgaa agtgagcccc acccccgcca
     5701 cgaaaaagag cagaagcgcg gtcagcaggc tgccctcggc gagcaggccg gcaagggccc
     5761 gttcctccgt ctgaaccgtc gcagcggggc tgccaggcgc gctgccggca tctccgccgg
     5821 atatcgcgcc cggggcgccc ggcattgtca gggtcaccca ccgggtctcc gggggatagc
     5881 aaagacccag atccgcgcat ccctgatagc cgatgcccac ctccacggtt gcgccgccct
     5941 gaacgggcag ccgggcagtg gctctgccgt agtacacctc cacctcgccg aagtactcgt
     6001 cgaccttggc cttgcctggc gggatctccg gggcgccgag aacggcaatc gccccaccac
     6061 ccgccggctc gcgggcctcg aaggtgaatc ggtgccggta gaggtagtag ccgtccgcga
     6121 tgtcccagcg aacgagtatc gcgccgtcgg gcccgacctc ggcgctcatg gcaaacgcct
     6181 cgtccaccgg gagaaacgtc ggctgctcgg atagccaccc ggcggccccc gtggagatgt
     6241 cgtccagggc cgacgcggcc gcaccgggcg cggccagcag caaagtaaaa ccaatcgccg
     6301 cggcgccaag ccccggccag aatcgcctca ttgttcgacg gactctcatc acctcaattc
     6361 tatccgacac ctcgaccgca ttggagttcg cccgcagacc cggcaatata gccgttccct
     6421 tggcccgcag aagtgaactg gtcggttgct tcccagtgaa cgaacactcc cagcgctctg
     6481 gctcgccctg atgctctggg tggtggacgc cgctggactc tcggcgcagg ccggacgtga
     6541 ttccttgccc gaaggagcgc gggccgaccc tctcaccgag cccgcgcccg ccccggcccc
     6601 tcacgcggcc gagatagacg cgctgctgct gcaggccaac gacgcaatgg aacggggaca
     6661 gtacatcaac cccgcccgcg gaagcgccct gggcctctac gaccgggtac tggcgctcaa
     6721 tccgggcaat gccgaggcgc gcaggggcct ggagctcatc gctcagcatt atctcgacct
     6781 cgccagcgcc gcagtaaggc ggcgtcagct gacccgcgcc cggtccatgg tcgactgggc
     6841 cagaatcgtg gacgccgaac atcccggcat cgccgcggcc gaggcgcaga tccgcctgct
     6901 cgcagaggcg aggcgcgtgc gcctgccggt ggcaggcagc cagctgcgcg atcgcagcga
     6961 cgcgctggct cggtcgttgg cggagctcgg atcacgcgcg cgcaacggaa actgcctggc
     7021 gatcatccgg tcgcccagcg acgcctcggg acgctggata taccagcaga tgagccgcgc
     7081 tccgggaact gcgcgcatcc gggccgacat cgagatcggc tcaccgccca tggtggaact
     7141 gctttgcttc gaaaacgacg cctgatgccc gatgccgcca tattggccgc cgcgcttgcc
     7201 gccgcgctgg ccgccgcgcc ggttgccatc ccggccagca gcgcgcccgc cctggcgggt
     7261 tccgagctgg tcgtgaccga tgcgcgggtc cgggcgctgc tgcccggcca gcgccatacg
     7321 gttgcctact tcgcgatcga aaaccgcggc cccgcaagcg ttaccctggt gagcgccgag
     7381 agcgcggccg ccggggccat ggagattcac gagtctcgcc gcgacggcga catgtaccgg
     7441 atgcggcgcc gagcagaggt tgagattcca gcccggacca ccgtacgctt cgacccgggc
     7501 gggctgcatc tgatgctgtt cgacgtccaa gcgctcggcg agcagatgga aatcgagctg
     7561 cgcacgagcg acgggcgccg cctgaaggca atctttaatc gcatagcagc gggagcggaa
     7621 tgaagatcaa ctggcgattc tgggcttctt cgaacgatgc ggacgtcacc gacgggaggc
     7681 tcgcaactgc cggtcgcatc gggctgggcg ccgttgccgc ctacctgctc gtctgcgtgc
     7741 tgctgggctg gtggtgggac aaggaacccg acgtgtttgc cgttcgcgag aacgcatcga
     7801 gccgcgcgga gctcggcaac gagccgctgg tcaccggtta catcaccacc gccaccctga
     7861 tccgcctggg cgaaaccctg ctcgacaaac gcggtggcta tctttccaac gacgtctttc
     7921 cgccgggcgt gtggatggac aacattccga actgggaatt cggggtgctc acccacatac
     7981 gggacgccac ccgcacctac cgcaacgagc tttcgcgctc ccagagccag tcggcagaag
     8041 accccgacat cgtcagagcg gagggcaagt tcttcatcga caacgcgagc tggttcatcc
     8101 ctcaagcgga gtcggaatat cgcgacggca tcaaagcgct ctcctcttac ctgcagcgcc
     8161 tggccgaccc cacccagccc gatgcccagt tctacgcgcg ggcagacaac ctccagcagt
     8221 ggctgcagat cgtcgaagcc cggctcggca gcctgtccca gcgcttgtcc gccagcgtgg
     8281 gcaaacggca gctcgacgtc tccctggccg gagatgctgc ggccagccag gccactgagt
     8341 ccccgcggga gcaggaggtc aagacgccgt ggctggaaat cgacgacgtc ttctacgagg
     8401 cccgaggcca gacctgggcg ctgctccacc tgctccgggc catggagcag gactttggcg
     8461 acgtgctgga caagaagaac gcccgagtca gcctgcagca gatcatccgg gagctcgagc
     8521 ccacccagga cacggtctgg agccccatca tcctcaacgg cgacgggctc gggttgctgg
     8581 ccaaccactc gctggtcatg gcgagctacg tctcccgggc gaatgccgcc atcatcgacc
     8641 tgcgggcgct tctctcccag ggctgaccgg cggcgggtcc catacaaatt cagacaaatc
     8701 ccgccgcgcg ctaggttctt attcgcagac cgctccgtta gaattcgcgc cgaaccgagc
     8761 gctgcctccg cgcgtatcgt gcagcaacaa gcacggggtg gagcagatcg cccgcgttcc
     8821 acacatggtc atcgagttgg ggaatccaat gaacaatatt gcgctgatac cgccaatatt
     8881 cggtatcgtc ggcctgatcg tagccttctt catcttcagc ctcgtgaagc ggtacgacga
     8941 gggcaccgag gaagtcaagc ggatcgggga cgccattcac gagggggcca tggtcttcat
     9001 gcgccgggag tacacgatgc tggcggggtt cgcggccgcg ctgatcgtca tcctctacat
     9061 cgccctcgga acagaaacca ccatcgcctt catcgtcggc gcgctggcct ccgcgacggc
     9121 aggctggatc ggcatgtaca cggcgaccaa agccaacgtc agaacgacta cggccgccca
     9181 caccgccggc gcggcccagg cgctgtccgt ggccttcttc gggggatcca taatgggcct
     9241 caccgtcgcg tccatgggcc tgctcgggct cggcatcctc tactacgtgt tcggcggcga
     9301 cccggagacc gcgcaccaca ttcacgggtt cggcatgggc gcttccaccg tcgcgctctt
     9361 ctcccgggtc gggggcggca tctacaccaa gagcgccgac gtcggcgccg acctggtggg
     9421 taaggtcgaa gcgggaatcc ccgaggacga cccgcgaaac cccggcgtga tcgccgacaa
     9481 cgtgggcgac aacgtgggcg acgtcgccgg catgggctcg gacatcttcg agagctattg
     9541 cggctccatg atcgccacca tcgccatcgc ctcgaccctc gcgctggcag cactgacgga
     9601 tctggcgccg ggcatggacg agggctccgc ccgggcggcc ctcatgggcc tccccctcat
     9661 tctcgcttcc acgggcctga tctgctcgct ggtcggcatc gcgctggtga ggctgatg
//
LOCUS       DBNHKV010000080         5225 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_650370, whole genome shotgun sequence.
ACCESSION   DBNHKV010000080 DBNHKV010000000
VERSION     DBNHKV010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_650370"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="AC16PP_02385"
     CDS             complement(<1..758)
                     /locus_tag="AC16PP_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480501.1"
                     /translation="MRFTFLFLSILLAATLAGNASAASYRSAPSEPVTNGFDPVQRAA
                     ERRALYDQLWVEKLVVTEPGVRVRLNDEDLRALSETVSQGNRQVPAERRQRIGVVKPV
                     AHGVDFAGMGAAPTRPKSFAGGQLLASGGGLTWTALVRAEGATASRLHFTGVNLPEGA
                     ELFVFNERGQAYGPYTGSGRNGNGAFWSEALIGTDTYVHLRVPGGLSAAELRGLRFVI
                     NDVGHIGPNYRYVDVWTSCEGNANCVKRASSSDPG"
     gene            986..1744
                     /locus_tag="AC16PP_02390"
     CDS             986..1744
                     /locus_tag="AC16PP_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480502.1"
                     /translation="MKVSTIITAIAMLFVLSCTTVAQETGRISEAFACNFKDGKGSKD
                     LWAAVDFWNEQMDAMGAGPLDDYFAAILTPYRATTGADFFWLGSSSNLNSFAAGGHAY
                     VNSPQGQAADARFEEISRCDSNVFLSETLYDGLGAPDDVDPSAFLETFACTLNEDATM
                     DDVTAAEEGWIAHAKSAGAKVDVIRWTPLYANTSADVYYLVVSADMPSFGAYRTAWLT
                     SEGGQAADRAFDEITNCESGLMIGTRIRVPAPVD"
     gene            complement(1892..2641)
                     /locus_tag="AC16PP_02395"
     CDS             complement(1892..2641)
                     /locus_tag="AC16PP_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480503.1"
                     /translation="MGVLAHYLERAGVATTQISLIRLHTEKIRPPRALWVPFQLGRPL
                     GDPEDPAFQTRVLRSALALLERTDGPVLEIFPDDAPSSAATAADAWVCPIGLPAGAHD
                     PDPEAALLDEIAAIQPWYELARERHGRTTVGASGMDIAEVAIRLNGLLDPQGAGSFEE
                     AGESRKQLHHLVGDLRAYYTEAATAQPGRTGLTPSSREIENWFWEETEAAQMLLRLRD
                     RLSDSADPELAFFGRLLTVPFSHQHLAPAGS"
     gene            complement(2660..3118)
                     /locus_tag="AC16PP_02400"
     CDS             complement(2660..3118)
                     /locus_tag="AC16PP_02400"
                     /inference="COORDINATES: protein motif:HMM:NF019003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HIR6480504.1"
                     /translation="MATAENVDPKLWQILQKMKTPDFEPTTPLSGPALSKRRVAIVTT
                     AAIQRAGERPFSAHASDYRVIPRDAADSLTIGHISVNFDRSGFQRDYNVVFPIDRLEE
                     LAQRGEIGSVADYHYAFLGSSNPLKMEAAAGQVAGLLKQDRVDAVLLTPV"
     gene            complement(3167..4777)
                     /locus_tag="AC16PP_02405"
     CDS             complement(3167..4777)
                     /locus_tag="AC16PP_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR6480505.1"
                     /translation="MSKGSRKRLTLGLVLGVGALGVALGWPIPDSERWEFGPDPKLAA
                     GKTSYLSALAAETFDKRPNVLLIVTDDLGRYDLSAYGHTAAHTPNIDALARDGVQFLS
                     AFATAAICAPSRAALLTGRYQNRFGFESQPMQRYVRNLGEYLGFRYLIDTDEMQPFLL
                     DTYPNAEQRLRQGLPASEISLGDALDAAGYATGIFGKWHLGYGPENHPQAFGFEQQYG
                     FTEAFSLYANEGAPGIVEHRHELFWEEHIWNMGREGPSAITRNGEPIAEARYLTDAIV
                     EETKRFVSDARAEERPFFAYVPFSAPHTPFQARSEDFLAVRGAADHNERVYLAMIQRL
                     DWAVGELVRYLESQGMLDDTLIVFTSDNGGAAYTGATDNGPLRGGKFTHFDGGLAVPL
                     ILAQRDVLAPRSVRRPVILTDLFATILRHCGLELPSDRTYDSVDLLTEPDSDTERSLF
                     WRSEFNRTVRRGSWKLVQNRRDGQLQLFNLADDPGEQHDLAEDQPAVVAELVTDLDAW
                     EQEMAPALWPRVMNYHYEDAIGSYWFAI"
     gene            complement(4790..>5225)
                     /locus_tag="AC16PP_02410"
     CDS             complement(4790..>5225)
                     /locus_tag="AC16PP_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6480506.1"
                     /translation="CDLRVAARDAVFGVYCRRWGVPLVDGGTIRLSRLIGHSHALDLI
                     LTGRGVSGDEAKAMGLANRIAPPGEALEAAMKLARSLTEFPQLCLRNDRLSSYRQWSM
                     PLEDALREETRLGLEVIRSGETHKGAARFADGKGRHGDFGDI"
BASE COUNT          875 a         1649 c         1785 g          916 t
ORIGIN      
        1 ccagggtccg aagatgaggc tctctttacg cagttggcat tgccctcgca cgacgtccac
       61 acgtcgacgt agcggtaatt gggcccgatg tgccccacgt cgttgatgac gaagcgcagc
      121 ccacgcagct ccgccgcgct cagcccgccc ggcacgcgca ggtgcacgta ggtgtcggtg
      181 ccgatgagcg cttcggacca gaacgcgccg ttgccgttgc ggcctgagcc cgtatagggc
      241 ccgtaggcct ggccgcgctc gttgaagacg aagagctcgg cgccttctgg cagattgacc
      301 ccggtgaagt gcagccggct cgcggtggcg ccctcggctc gcacgagcgc cgtccaagtg
      361 aggccgccgc cgctggccag gagctggccg ccggcaaacg actttggccg cgtgggcgcg
      421 gcacccatgc cggcgaagtc cacgccgtgg gcgacgggct tgaccacgcc gatgcgctgg
      481 cgtcgctctg ccgggacctg gcgattgccc tgggacaccg tttcgctcaa agcccggaga
      541 tcctcgtcgt tgagccgaac ccggaccccc ggctccgtca ccacgagctt ttcgacccag
      601 agctggtcgt agagcgcgcg cctctcagcc gcacgctgaa cggggtcgaa accgttggtc
      661 acgggttcgg agggtgctga tcggtagctc gcggcgctgg cgttgccggc aagggtggcg
      721 gcgagcagga tactcagaaa caggaaagta aaacgcacac ctgacaaaac tgatgattgc
      781 aatgatccaa atgtagcaga cctcgccacg agttgggaca tcgttttgtg acgatttgca
      841 aggtttcgag gctgcgaatc tgcagcgtat catcaccagg ggaagagaga cgtcgaccgg
      901 cggtccggaa gcggacggcc tttgggttag acgcgctgtt cccgcatgag tgacgaaaga
      961 tgaacgggac aaatgggggg aaccaatgaa agtctcaacg atcatcaccg cgatcgccat
     1021 gctgttcgtg ctgtcctgca cgacggtggc ccaggagacg ggccgcatca gcgaggcctt
     1081 cgcgtgtaat ttcaaggacg gcaaaggcag caaggacctg tgggccgccg tggacttctg
     1141 gaacgagcag atggatgcca tgggagctgg cccgctggac gactacttcg cggcgatcct
     1201 gacgccgtac cgggcaacca cgggggctga tttcttctgg ctcggatcct cgtcaaatct
     1261 caattcgttc gccgccgggg ggcatgccta cgtcaacagc ccccagggtc aggcggccga
     1321 cgcgcgcttc gaggagattt cgcggtgcga ttccaacgtc ttcttgtctg agactctcta
     1381 cgacggcctc ggcgcgccgg acgacgtgga tccttccgcg tttctggaga ccttcgcgtg
     1441 caccctgaat gaagacgcta ccatggacga cgtgacggcc gccgaggagg gctggattgc
     1501 tcacgccaaa tccgcaggtg cgaaagtcga cgtgatccgc tggaccccgc tctacgccaa
     1561 cacgtcggcg gacgtctact acctggtggt cagcgcggac atgccgtctt tcggcgcgta
     1621 ccgcacggcc tggttgacgt ctgaaggtgg ccaggctgcc gatcgggcgt tcgatgagat
     1681 caccaactgc gagtccgggt tgatgatcgg cacgcgcatc cgcgtgccgg cgccggtcga
     1741 ttaacgccgg cgggtttcga ttctcctcga cgaaaggcgg gccgcggcct cggcggcccg
     1801 cgaactaggg ggaaagcccg atagggtccc gctgcggatc cagcaccatc atgactggct
     1861 gggtcacatc atcggcaata cctgagcctg ctcagctgcc ggccggcgcc agatgctggt
     1921 ggctgaaggg tacggtcagc agccggccga aaaaggcgag ctccgggtcg gcgctgtcgg
     1981 acagccgatc gcgcaggcga agaagcatct gagccgcctc ggtctcctcc cagaaccagt
     2041 tctcgatctc tctgctcgat ggggtcaggc cggtgcggcc cggctgtgcc gtggcggctt
     2101 cggtgtagta cgccctcaga tcgccgacga gatgatggag ctgcttcctg ctctctccgg
     2161 cctcttcgaa agagcccgcg ccttgcgggt cgagtaggcc attcagacgt atggcaacct
     2221 cggcgatgtc catcccgctg gctccgaccg tggtccgccc gtggcgttcg cgcgcgagct
     2281 cgtaccaggg ctggatcgcg gcgatctcgt ccagcagagc cgcctcggga tcggggtcgt
     2341 gcgctccggc aggcaggcct atggggcata cccaggcgtc cgcggccgtt gctgctgatg
     2401 agggcgcatc gtccggaaaa atctccagaa cagggccatc ggttcgctcc agcagcgcca
     2461 gggctgagcg gagcacgcgg gtctggaatg ccgggtcctc ggggtctccc agggggcgcc
     2521 cgagctggaa cgggacccac agcgccctgg gggggcggat cttttccgta tggagcctga
     2581 tgaggctgat ctgcgtggtg gccacgcccg cgcgttccag gtagtgagcg aggacaccca
     2641 cggtgcccgt gcaggcgggt caaacgggtg tgaggagcac ggcgtcgacg cggtcctgtt
     2701 tcagcagccc ggctacctgg cctgcggctg cctccatctt cagcgggttc gaggatccca
     2761 ggaacgcgta gtgatagtcg gcaacggatc cgatctctcc ccgctgcgcg agctcctcca
     2821 gccggtcgat ggggaacacc acgttgtagt cccgttgaaa cccggatcgg tcgaaattga
     2881 cggagatgtg cccgatggtc agggagtcgg cagcgtccct cgggatcacc cggtaatcgc
     2941 tggcgtgagc ggagaagggg cgctcgccag cccgctgaat ggcggcggtg gtgacgatgg
     3001 ccacccgtcg tttggacagg gctggacccg aaagcggcgt cgtcggctcg aaatccgggg
     3061 tcttcatctt ctgcaggatc tgccagagct tcgggtcgac gttttcagcg gtggccatgg
     3121 ccctcgtccc tcaaccgttc gaagtcgcgc gccgggggcc tcgggctcag atcgcgaacc
     3181 agtagctccc gatggcgtcc tcgtaatgat agttcattac gcgcggccac agggccggcg
     3241 ccatctcctg ctcccacgcg tcgaggtcgg tgacgagctc ggcaacgacg gcgggctggt
     3301 cctccgccag gtcgtgctgc tcgccgggat cgtcagcgag gttgaacagc tgcagctgac
     3361 cgtctcgccg gttctggacc agcttccacg agcccctgcg cacggtccgg ttgaattcgc
     3421 tgcgccagaa cagtgatctt tcggtgtccg agtccggttc ggtcagcagg tccacagagt
     3481 cgtaggttct gtccgagggg agttccagcc cgcagtgacg gagaatggtt gcgaacaggt
     3541 cggtcaggat gacgggtctg cggacggatc gcggtgccag gacgtcgcgc tgggccagaa
     3601 tgagaggcac cgcgagaccg ccgtcgaagt gggtgaactt gccgccgcgc aaagggccgt
     3661 tgtccgtggc gccggtatac gccgccccgc cgttgtcgct ggtgaagacg atgagcgtgt
     3721 cgtcgagcat tccctgggat tccaggtatc ggacaagctc gcccacggcc cagtcgagcc
     3781 gctggatcat cgcgaggtaa acgcgctcgt tgtggtctgc ggcaccccgc accgcgagaa
     3841 agtcctcgct gcgcgcctgg aaaggcgtgt gcggcgcgct gaacggcacg taggcgaaga
     3901 agggcctttc ctcggccctg gcgtcggata cgaagcgctt cgtctcttcg acgatggcgt
     3961 cggtcaggta acgggcctct gcaatcggct cgccgttgcg ggtgatggcg gacggaccct
     4021 ccctgcccat gttccagatg tgctcctccc agaagagctc gtggcggtgc tcgacgatgc
     4081 cgggcgcgcc ttcgttggcg taaagcgaga aggcttcggt gaagccgtac tgctgctcga
     4141 aaccgaaggc ctgcgggtgg ttttccgggc cgtatcccag atgccacttg ccaaagatgc
     4201 cggtggcgta gccggccgcg tcgagggcgt ccccaaggct gatctcgctg gccggaagcc
     4261 cctgccgcaa tcgctgctca gcgttgggat aggtgtcgag aagaaacggc tgcatctcgt
     4321 cggtgtcgat gaggtagcgg aatcccaggt actcgccgag gttgcgcacg tagcgctgca
     4381 tgggctgaga ctcgaaaccg aagcggttct ggtagcgtcc cgtgagcagc gccgctcgag
     4441 acggcgcgca gatggcggcg gtggcgaagg cgctgaggaa ctgcaccccg tcccgcgcca
     4501 gggcgtcgat gttgggggtg tgcgcggcgg tgtggccgta cgcggacagg tcgtatcgcc
     4561 ccagatcgtc ggtgacgatt aacaggacgt tgggtcgctt gtcgaaagtc tccgccgcga
     4621 gcgctgacag atagctcgtc ttgcccgctg caagttttgg gtcggggccg aactcccagc
     4681 gttccgagtc gggaatcggc catcccagag cgacccccag agccccgaca ccaaggacca
     4741 gcccgagcgt cagccgtttt ctgctcccct tgctcatgac caatgggcgc tatatgtcac
     4801 cgaagtcgcc gtgacggccc ttgccgtccg cgaaccgggc agcgcccttg tgggtctcgc
     4861 cggatcggat gacctccagt cccagacgcg tctcctccct gagcgcatct tccagtggca
     4921 tcgaccactg gcgataggaa gaaagccggt cgttgcgcag gcacagctgg ggaaattccg
     4981 tgaggctccg ggccagcttc atggccgctt cgagggcttc acccggcggc gcgatgcggt
     5041 tggccagacc catggctttc gcctcgtccc ccgagacgcc ccggcccgtg aggatcagat
     5101 ccagcgcgtg gctgtggccg atgagccggg acagccggat ggtgccgccg tccaccagcg
     5161 gcacgcccca gcgccggcag tagacgccga agacggcgtc ccgggcagcg acgcgcaggt
     5221 cgcac
//
LOCUS       DBNHKV010000081        23295 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_225074, whole genome shotgun sequence.
ACCESSION   DBNHKV010000081 DBNHKV010000000
VERSION     DBNHKV010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 23295)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 23295)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23295
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_225074"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2257)
                     /locus_tag="AC16PP_02415"
     CDS             complement(<1..2257)
                     /locus_tag="AC16PP_02415"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HIR6480507.1"
                     /translation="MLSDLRVLDLSTDLGLMCGQLLADMGAEVQQWVLPEDAARLDTC
                     YWQAYTLGKGVQLIDWQADPEAVRRQVGCVDVIIESAPPGLMARLGLGYEELARINPE
                     VVHVSITGFGSTGPKADYEFSDLISAAAAGLVYVTGSEGQAPVRTTCPQAHGHASSDA
                     AVGVLIALFERARTGRGQHVDVSAQQSTTLALLNRALDGPVGQAKAERSAYGMTINGV
                     HVQNQFQARDGWVVCLPGVLPPIAAFMERLMAWVFEEGLCREEDLRWDWGGVALQIMQ
                     GQVTPEQWAPVTAGMEALIAGRTKAELMSEAISRRLLIAPVFSVADLLDSDHLAQRGF
                     LVEGGAGLRLGPFARLSESPLPLTESGPRFWQERSAFDARPLAASPSGRDESGGDTEG
                     GGGPLAGLKVLDLFWVVAGPGATRMLADYGATVVHVESTRRQDMVRNVPPYIDGVPDP
                     ERAACHHSTNTNKLNLTLDISREEGRAVLADLIRWADVFTESFAPGAVARMGFDYATV
                     RTLNPNIIMISSCLMGQTGEWSGYAGFGNNGSAVSGFHALTGHPGQPPTGVFGPYTDF
                     TSVRFNALAILGAVMHGRRTGNGQYIDMSQGEAALHFLAPECLRYLRDGRVRGAEGNR
                     DEHFAPQGVYPTAGFDRWLALSVRSDAEWRALCALAGFDDLSNMSVEERAAAHDELDR
                     RLETWMETRPGEELEAELQSAGIAAHRTLDTHELGDDPQLVHRGHFIPVAHREFSNAV
                     VESSRLTLSASR"
     gene            complement(2247..3113)
                     /locus_tag="AC16PP_02420"
     CDS             complement(2247..3113)
                     /locus_tag="AC16PP_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480508.1"
                     /translation="MVAFRRCLVIALWCLSAHALGYDGRIHQDLTFLAAKHFNRCTAG
                     TEVPPLTPLQIRYLAKTNVGLADRNAFARMFNWRYYDRAAEAEHSVMWAIDTRFHEHF
                     NEILARIDKADSMGKLYSEVGRIINYLQLVSSPPHTVPVFTSRFWRFSFSDRFDGFPV
                     DVEAVGQAIGEDCSFLAAEEEYAGVLRSIAADTIAAVRAPIEGMPATWEAFWEIADDP
                     EKFGDYGPAGNSFGRKTEFGCAQQRCVLLRADPLYAAFAAQRHAAAVKATLAAMLIVQ
                     RSQIATEVAGAQ"
     gene            complement(3100..3603)
                     /gene="folK"
                     /locus_tag="AC16PP_02425"
     CDS             complement(3100..3603)
                     /gene="folK"
                     /locus_tag="AC16PP_02425"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR6480509.1"
                     /translation="MVLVGLGSNRGDSVGIVRAAMGRLRGFADGDTRCSDLWRTSPVD
                     CPPGSEDFINAAVSFRAAPGLTPESLLADLKALEAEYGRTGQRLRNAPRELDLDLLLF
                     DAETRDTADFVLPHPRAADRLFVLAPAAQVAPDVVWPEIGLTIRELLDELDSDERVVR
                     LEAHGCL"
     gene            3764..4846
                     /locus_tag="AC16PP_02430"
     CDS             3764..4846
                     /locus_tag="AC16PP_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HIR6480510.1"
                     /translation="MKAAVLREINKPLEIEDIQHGDPGPREVLVRTVAAGVCHSDLHF
                     QNGSYPYPLPAVLGHESAGIVEAVGSDVNYVKPGDHVITCLSAFCGHCEYCLTGHMSL
                     CQEPELQRPMDQPQRLNKGDENIWQFLNLSSFAEYMLVHEHAIAKIREDMPLDRAALI
                     GCGVTTGVGAVIHTAKVEPGSTVAVIGCGGIGLSAINGAAIAGAARIIAIDTVESKLD
                     LARKFGATDVVNARESDVIEAVREMTGGGVHYSFEAIGLKQTAEQSFKILQRGGTATV
                     IGMIPVGTNIELHGPEFLMERKMQGSNMGSNRFRVDMPRFVEFYLQGRLHLDDMISRR
                     IRLEDVNDGLAALETGELARSVIMFD"
     gene            complement(4867..6228)
                     /locus_tag="AC16PP_02435"
     CDS             complement(4867..6228)
                     /locus_tag="AC16PP_02435"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HIR6480511.1"
                     /translation="MRHTLKCAVFGWLLLGSLTAAAALPLGLPGGEQLPSLAPMLDQA
                     TPAVVNIATYTTVQVRNPLLEDPFFRRFFNVPDQRRYRRTQSAGSGVVIDAGKGFIVT
                     NNHVVERADEISVTLADGRSMTAELVGSDPQVDLAVLKVEADDLQQIGFADSGELRVG
                     DFVVAIGNPFGLRQTVTSGIISALGRSGLGIEGYEDFIQTDASINPGNSGGALVDLAG
                     RLVGINTAILAPSGGNVGIGFAIPSNMVRAIMDQLIEHGEVRRGYLGLAVQGLNVDLA
                     EAFGVDRREGVVVVEVEPDSAAESGGLQAGDIITRVGDREIGKIADFHSQAAVTFVGD
                     QVRIEVLRDGQPQQVTLKIGEDELEKVRGRRIDRRLAGTQLQNFRNQDEPGMGAGVLV
                     LDIDPDSDAYRFGLRPGDILVAANRRAIVNLAELREVARLDDGQLLLRVYRSGQFGYV
                     AIR"
     gene            6359..6787
                     /locus_tag="AC16PP_02440"
     CDS             6359..6787
                     /locus_tag="AC16PP_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR6480512.1"
                     /translation="MPIKNVFEAIQREKANIENLSVEELKQALADDPDLLLLDIREIQ
                     ERVDLGTIPGSVHVPRGMLEFWADPASPYFRDYFQEDRRTVVFCAGGGRSVLAVRALQ
                     DMGFTDVAHLEPGFGGWKKAAEPIEDVASSSRWVRRADDD"
     gene            6863..6938
                     /locus_tag="AC16PP_02445"
     tRNA            6863..6938
                     /locus_tag="AC16PP_02445"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6896..6898,aa:Gly,seq:ccc)
     gene            complement(6942..8723)
                     /gene="pabB"
                     /locus_tag="AC16PP_02450"
     CDS             complement(6942..8723)
                     /gene="pabB"
                     /locus_tag="AC16PP_02450"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - aminodeoxychorismate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008153 - 4-aminobenzoate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HIR6480513.1"
                     /translation="MSTDVIVYDAVAQQWLRFCDPVELLIADREDQVLDVVAEIESRT
                     VGDGLFAAGFLTYEAAPGIARELVTHRPHRLLPLAWFGLFRRVLPSADLPLAPGDCLG
                     GWSASVTRKDYDSAIARIRDYIAAGDTYQVNYSFRLRARLNDSPAALFANMVSSQGPG
                     LAAFIDTPGFAVASASHELFFARDGDELVSRPMKGTAPRGLRDQTDRDQAGWLAASAK
                     NRAENLMITDMVRNDLGRIARIGSVVADDLFALERYPTVWQMTSTVRARSTASLPDVM
                     GALFPAASITGAPKRRTMEIIRELEHEPRGIYTGTIGFVRPEGRCQFNVAIRTAAIDK
                     TTSEAEYGVGGGIVWDSRAEDEYRECFAKARIVTGGRREFELLETLAWHPRDGFRRLE
                     EHLERLHSAADYFCWSLSTGKAQALLEAAASAYREPMRVRLLADRSGHVRLEASPLKP
                     LPEPYRVALSEPMEDLQSEFVYHKTTQRSVYDKALDAARSVLPDLDDVLLVNGDGQVT
                     ESTIANVAVEVDGRLLTPELGCGLLPGIERQVLVADKILEEARVPVSLLVEAERIWLL
                     NSVRGMWPATLVATEQGGSDLRSEFPG"
     gene            complement(8845..9828)
                     /locus_tag="AC16PP_02455"
     CDS             complement(8845..9828)
                     /locus_tag="AC16PP_02455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6480514.1"
                     /translation="MSSKLTPGPPALTWLILGSLLAVLSLPAISSEPARPLSAHHVIV
                     DGVPLAVWRKAPASAKRVVLLVHGRTWSARPNFDLNVPGEDLSLMDGLVEEGIATYAL
                     DMRGYGDTPRDASGWLTPSRAAADVIAVLEWIAQRNPGLPGPYLFGYSRGAMVAQLAA
                     QQQPGRISGLIVFGYPLRQDSAVNPPGLTTEPARIANSAADARSDFIAPGAARRETID
                     AFVESALKHDPVRVDWRAYPEWLALDAARVTVPTLLVDAEHDPLTDPKVLSRFFAGLA
                     TADKAWVSIPGADHAAFLEEPRDYFRRAVAAFMLGERAAVEVISADLLDFD"
     gene            9968..10741
                     /locus_tag="AC16PP_02460"
     CDS             9968..10741
                     /locus_tag="AC16PP_02460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6480515.1"
                     /translation="MDVSGKIVVVTGGASGIGRAMAQRFHAEGAGKVIVADLDGDGAR
                     RVADEIDGSSMQVDVGREADLVALIEHAESEFGPIDLFCSNAGIGIGKGLDEPDEVWD
                     TIWKVNTMSHVYAARHMVPRMKARGGGYLLNTASAAGLLTQIGSVTYAVTKHAAVALA
                     EWIAITHARDGIKVSVLCPQAVRTPMTADGPGVAGVDGMIEPEEAAECVIEALREERF
                     LVLPHPEVATYIQRKTSDYDRWLNGMRRLQDLYVAEEGE"
     gene            complement(10791..11588)
                     /locus_tag="AC16PP_02465"
     CDS             complement(10791..11588)
                     /locus_tag="AC16PP_02465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR6480516.1"
                     /translation="MYQFNYHRPASVEEAVGLFESMDDGIYMAGGQTLIPTMKQRLAA
                     PTDVIDLSGIEALSGIEVSADRVSIGAFTVHDAVASSPEIRATLPVLAFLAGQIGDGQ
                     VRNRGTLGGSVANSDPAADYPAALIGLGATVHTQTRSIEADDYFKDLFETALNPGELI
                     TRIDFPVPTRAAYRKFPNPASRYAVVGVLVADFGGKVRVGVTGAGPCAFRATAIEELL
                     SRNLDPSALDGVEVPDAGFNNDLHASAEYRAHLVKVMAKRAVADLLS"
     gene            complement(11597..13945)
                     /locus_tag="AC16PP_02470"
     CDS             complement(11597..13945)
                     /locus_tag="AC16PP_02470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6480517.1"
                     /translation="MSEAAATGIGASVRRREDVRFITGSGRFTDDLTQPGQTYAVFLR
                     SPYARARIGEIDVSEALATEGVIAVLTGRDMAADGLGDLPCGWLVKQKDGSDMISAPH
                     PPLATEVANYVGEPYGLVIAESLAAARQAAECVMADFEELDAVVDLATAVDSSQIHEG
                     VPGNRAYDWEIGDREATEAALAGAAHVTRIDLTNNRLIPNAMEPRAALGVYDPAGDDY
                     TLYTTSQNPHLERLVLSAFVQVAPEHKLRVIAPDVGGGFGSKIFIYAEETAVIWASRR
                     VGRPVKWTAERSESFLADAHGRDHITHAELGLDAEGNFTALKVQTSANLGAYLSTFGS
                     SVPTYLYGTLMAGQYRTPNIYVEVQGIYTNTAPVDAYRGAGRPEATYVVERIVETAAR
                     ELGTDPAELRRRNMIRPDQFPYETPVALVYDTGDYEAGLDRALALVDYAGFPARRAAS
                     EAAGKRRGIGLACYIEACGLAPSQLAIQLGAGVGLYESGEVRVNPTGSVTVFTGSHSH
                     GQGHETTFAQVVSDRLGVPFESVEVVHGDTGRVEFGLGTYGSRSLAVGGSALIAATDK
                     VIAKGKRIAAHMLEAQESDVEFADGNFVVRDSNQSLSFGEVAFAAYVPANYPEDLEPG
                     LSEKAFYDPENFTYPAGTHIAEVEIEEETGVVRLVNFVAVDDFGHVINPMIVDGQVHG
                     GLAQGAGQALLEHGVYDDYGQLQTGSYMDYCMPRADDVPDFVVDTTVTPCTHNPLGVK
                     GCGEAGAIGSPAAIMNAVTDALGVNDISMPATAEKVWRALRA"
     gene            complement(13955..14413)
                     /locus_tag="AC16PP_02475"
     CDS             complement(13955..14413)
                     /locus_tag="AC16PP_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR6480518.1"
                     /translation="MKVALNVNGTDRELEVPDNTLLVDLLREHLRLTGTHVGCDTSQC
                     GACTVHLNGRSVKSCTVLAAHAEGAEVVTIEGIGAPDDLHPMQEAFRDCHALQCGFCT
                     PGMIMSAIDLVNQSDDLDNQAVREGLEGNICRCTGYHNIVRAVLQAAEKM"
     gene            complement(14453..15004)
                     /locus_tag="AC16PP_02480"
     CDS             complement(14453..15004)
                     /locus_tag="AC16PP_02480"
                     /inference="COORDINATES: protein motif:HMM:NF017999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6480519.1"
                     /translation="MEQFGEYRISAARAMVWEGLNDPEVLGRCIQGCLSMERTSDEQF
                     DASVKAKVGPVSATFQAQLMLSEIDAPSSYTLTANVKGGAAGFGKGTAKVSLLEDGAS
                     TLLRYQVSANVGGKLAQVGSRLIDAAARKMADDFFAEFGREVGGAEPEALAAVAEAPV
                     AGGRWKIWVAVAAIVVIILLLMV"
     gene            complement(15064..16674)
                     /locus_tag="AC16PP_02485"
     CDS             complement(15064..16674)
                     /locus_tag="AC16PP_02485"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spinster family MFS transporter"
                     /protein_id="HIR6480520.1"
                     /translation="MQQNEPSDSSGESADGVGGRYANYVLIVLIIVYVFNFIDRQILS
                     ILAEEIKADLGISDADIGFLYGTAFAVFYAVFGIPLARLADTWIRKDLIAIGLGFWSL
                     MTALSGTARNFASLATFRFGVGVGEASASPAAFSMLSDYFSPRVRATVLAIYSSGVYI
                     GAGIGVFLGGIIVDSWNDAFPDPVLAPFGLKAWQAAFFAVGLPGLLMAIWVWTLREPQ
                     RGVSEGLVVPTHPHPFRETARSLVTVLPPFTVVSLWVSGGARTVAVNLGLALLIAAVG
                     YGANLATGTPNQWIALGIGVYAVVSWAQGLKLSDPVAFGMIFGSRAMVLLTLGFPCLA
                     FVTYGVGFWSPPYLLRAHGVNLAEAGTLLGLGSALGGWIGVTLGGIVADRLRARSVNG
                     RIYIGVASPFLAAPAAIWFVLTDNVWVAYFCAFVFSMFSPMWLGAAASTVNDLVLPRM
                     RAVASAYYILMLTFIGLALGPYLIGQVSDIYLASGLDDGQALGRAILWGCGMLGVSLL
                     FLAGAMRYLGAEEANRLDRARALGEPMD"
     gene            complement(16767..17432)
                     /locus_tag="AC16PP_02490"
     CDS             complement(16767..17432)
                     /locus_tag="AC16PP_02490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6480521.1"
                     /translation="MELYDVMRTTFAAREFLDEPVTDGDIRNILDNARFAPSGGNRQG
                     WRVIVIREAETREAMLPMIMPTFQRYVAQVQAGEAPWNTINETRLTAEEIAATEPPEA
                     FIRQVLAAPVLLLILVDLSVVASFDAQLDRVGVISGASIYPFVWNVLLAARNEGFGGT
                     LTTFIAGREGEIRRLLGIPDHLAVAALLPIGRPVKQLTRLSRKPVDEFAMLERWDGAP
                     LGD"
     gene            17548..18030
                     /locus_tag="AC16PP_02495"
     CDS             17548..18030
                     /locus_tag="AC16PP_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6480522.1"
                     /translation="MEDQPNEVLMRGLLRAYYWMDESLQNGLQKAGYLPRTRTQTMIL
                     INISNGVTRAAELARVLGVSRQAIQQQINELERDDLVTQLPDPDDRRANRIVFSERGS
                     QLINAALATLRQSEQALAIRLGYETVNQLRRALMADWGPVIGERRAPGRKRSISNQND
                     "
     gene            complement(18131..19987)
                     /locus_tag="AC16PP_02500"
     CDS             complement(18131..19987)
                     /locus_tag="AC16PP_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6480523.1"
                     /translation="MLVALFSSGVTLAADHASRIEAHVESSATPWTGLQANDAEEDFT
                     FVVVADRTRGHRPGVFESAMPKVNLLQPAFVVSVGDLIEGYTDDPARLTGEWDEVEAF
                     VDQLDAPFFYAPGNHDMSNAVMSEAWRARFGPSFYHFLYKDVLFLVVNSELFGMVTNP
                     WVSVPGPWTQEEQMSYIERVLSRHADVRWTIVITHQPLWDVAEVHPDWLKVEALLGER
                     PYTVFAGHAHRYQRSLRHDRKYITLATTGGASPLRGALYGEFDHVAMVHMSSRGPTIA
                     NLMLDGIQPEDVTTEDSLRALRQLSDAVQVETEYLDGDLFTSGAVRYRIVNSGSAPLE
                     ATPIVLGNDVLRAQATPQAISVPPGGERRVALNLKSTHALAFESLPPARVTWSLRSML
                     LDRPVFVEIETALLPQRRFPLERISRLVRLDGDLEEWHLPYSVTRQGDIASPQTDPAD
                     ISFRFGIGYDDENLYLAAEVIDDSIVASGELTAMDQDALIVSVDARPDPDRSANQPLV
                     EAMASGATSRMVIELLTLAPGRPAPVLEMLDGVRDAIASAVVRTSGGYAAEIAIPGEL
                     LSRQQGREWQVVRVGIHANDFDESERGSKSLHWQPYRFGSAAVVGTGTFMRR"
     gene            complement(20160..20423)
                     /locus_tag="AC16PP_02505"
     CDS             complement(20160..20423)
                     /locus_tag="AC16PP_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014609.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR6480524.1"
                     /translation="MQLTIVDVFAERRYAGNQLAVVRDASGLSTAEMQDIAREMNFSE
                     TALVLESGSGHARARYFTPPPVRLGGEFDRALAGASSSIRAWR"
     gene            20553..21005
                     /locus_tag="AC16PP_02510"
     CDS             20553..21005
                     /locus_tag="AC16PP_02510"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR6480525.1"
                     /translation="MTGTVEGIYLAAKHGDSQRAVPAARLTIGVGLEGDRHANSKGGV
                     VTLIEAEAVERFIAETGLDITAADTGRNIATRGIGLNDLVGRQFRVGGVLMEGFELCE
                     PCATLGARLASRDVSAAAIVAAFTHSGGIRAFVRGTGTVAPGSAVEPA"
     gene            complement(21015..21605)
                     /locus_tag="AC16PP_02515"
     CDS             complement(21015..21605)
                     /locus_tag="AC16PP_02515"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6480526.1"
                     /translation="MTRQSGADGYPKQVRLPGGEEIEIRLMTGEDRDAVCEFARGLAE
                     EDLMFLRVDLTEPAVIDQWIRNLETGHSTSIVAYDGTGLVGYATVHRDPARWTRRVGE
                     IRVNVSPAYRAKGLGRTLTSQIFDLARSLGLRKLMANMTVDQSGAQAAFRRLGFVPEA
                     LLADYVEDRSGTPRDLVIMSYDIEGHTDQVAEPLRV"
     gene            complement(21625..23130)
                     /locus_tag="AC16PP_02520"
     CDS             complement(21625..23130)
                     /locus_tag="AC16PP_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR6480527.1"
                     /translation="MRLTSHDASFLYSESASGPMHTASIFVLEGELPFERVFSHFQAR
                     MHLIARYMQKLAFVPFNLAHPKWVDDPDFDLANHVVPHSVPEGSTLEEAVDLLLAVNE
                     GLLDRNRPLWKTYVVDGVPGKTLMLQQVHHAMIDGVSGIQLTMVQFDFDRDAPDPPPG
                     EPRQPATPPSAMDLVGEVVRESAERLSVNPLAGVHTDRKSFELLRNAGRAFSRFFTEP
                     AITAPWNAGLVGPKRRLRWTSHPFSEFREIRRAFGGTINDVVLAMVSEGAARYLDRRR
                     EETEGKYLRIMCPVSVRTEDEAGSLGNRVSAIFPLLPAWSMDAAERLRAVIGETERIK
                     RDEEAQALTFLSETMPSVPAVAMAPTLLVGTRFDPTALAGRMPLPVVPNLGTRLPLYG
                     FNFTCTNVPGVQVPQYLAGHEISNIIGILMLTGTLGYGMAVVSYNQNMRFNFVCEPRL
                     MPDLDVMVEAVEQVYQELLEEARRVNEPPAPPSRGGRSEKASRKSYDSPAA"
BASE COUNT         4186 a         7813 c         7506 g         3790 t
ORIGIN      
        1 cgcggcttgc cgacagggtc agccgtgagc tttccaccac ggcgttcgag aactcccggt
       61 gcgcaacggg aatgaagtga ccccggtgaa ccagctgggg atcgtcgccg agctcgtggg
      121 tatccagggt ccggtgggcg gcgatgccgg cagattgcag ctccgcttcc agctcctccc
      181 cggggcgggt ctccatccac gtttccaggc gccggtcgag ctcgtcgtga gcggccgccc
      241 gttcctcgac cgacatgttc gagaggtcgt cgaatcccgc cagggcacac aacgcgcgcc
      301 actcggcgtc gctgcgcacc gaaagcgcca gccagcgatc gaacccggcg gtcgggtaga
      361 cgccctgggg ggcgaagtgc tcatcgcggt tgccctcggc acccctgacc cgaccatcgc
      421 gcaggtaacg caggcactcg ggcgcgagaa agtgcagcgc cgcctccccc tgggacatgt
      481 cgatgtactg gccgttgccg gtccgacggc cgtgcatcac ggcgccgaga atggccaggg
      541 cgttgaagcg cactgaggtg aaatcggtat aggggccgaa gacgcccgtg ggcggctggc
      601 ccggatggcc ggtgagcgcg tgaaagcccg acactgccga gccgttgttg ccgaaccccg
      661 cgtagccgct ccactccccc gtctggccca tcaggcagct cgagatcatg atgatgttcg
      721 ggttgagcgt cctcaccgtg gcgtagtcga agcccatccg ggccaccgcg ccgggggcga
      781 aggattcggt gaagacgtcg gcccagcgga tcaggtcggc aagaaccgca cgaccctctt
      841 cccgactgat gtccagcgtc aggttgagct tgttggtgtt ggtgctgtga tggcaggcgg
      901 cgcgctccgg gtccgggacc ccgtcgatgt agggcggcac gttcctgacc atgtcctgcc
      961 gccgagtgga ctccacgtgc accacggtgg ccccgtagtc ggcgagcatg cgcgtggcgc
     1021 cggggccggc caccacccag aaaaggtcga ggaccttcaa tccggccagc ggcccgccgc
     1081 ccccttcggt gtccccaccc gactcatccc ggccggacgg ggaggcagcc aggggtctgg
     1141 cgtcgaaagc agacctttcc tgccagaatc gcggcccgct ttcggtcagc ggcagcggcg
     1201 actctgacaa acgggcaaac ggcccgagcc gaaggcccgc tccgccctcc accaggaagc
     1261 cgcgctgagc gaggtgatcg ctgtccagaa ggtccgctac agagaacacc ggagcgatga
     1321 gcagccgccg gctgatggcc tcggacatca gctctgcctt ggtgcgccca gcgatcagcg
     1381 cttccatgcc ggcagtcacc ggggcccatt gctccggggt aacttgcccc tgcatgatct
     1441 gaagggccac gccgccccag tcccacctga ggtcttcctc gcggcaaagg ccttcttcga
     1501 acacccacgc catcagccgc tccatgaaag cggcaatggg tggaagcaca cccggcaggc
     1561 ataccaccca gccgtcacgg gcctgaaact ggttctgaac gtgcacgccg ttgatcgtca
     1621 tgccgtaagc gctgcgctcc gccttggcct ggccaacggg accgtcaagc gcccgattga
     1681 gaagcgccag cgtggtcgac tgctgggcag acacatcgac gtgctgaccg cggcccgttc
     1741 gcgctcgctc gaagagcgca atgagcacgc ccaccgccgc atcgctcgag gcgtgcccgt
     1801 gggcctgcgg acacgtcgtg cgaacgggcg cctgcccttc gctgcccgtc acgtagacca
     1861 gccccgctgc agccgcggaa atcagatccg agaactcgta atccgccttc gggccggtgc
     1921 ttccgaaccc ggtgatggag acatgaacga cctccgggtt gatgcgcgcg agctcctcgt
     1981 agccgaggcc caggcgcgcc atgagcccgg gcggggcgga ctcgatgatc acgtcgacgc
     2041 agccgacctg ccggcgaacg gcctcgggat cggcctgcca gtcgatcagc tgaacgcctt
     2101 tgccgagcgt gtaggcctgc cagtagcagg tgtcgagtcg ggccgcatct tcgggcagga
     2161 cccattgctg aacttccgcc cccatgtcgg cgagcagctg cccgcacatc aacccgagat
     2221 ccgtggagag atcgagcacg cgaagatcac tgagcaccgg caacctccgt tgcaatctgg
     2281 ctgcgctgca cgatgagcat ggctgccagg gtagctttca ccgccgccgc gtgtcgttgg
     2341 gcggcaaacg cggcgtagag cgggtcggcg cgcaggagca cgcagcgctg ctgcgcgcag
     2401 ccgaactcgg tcttgcggcc aaagctgttg ccggccggac cgtagtcacc aaacttctcc
     2461 ggatcgtcgg cgatttccca gaaggcctcc caggtggccg gcatgccttc gatgggcgcg
     2521 cgtaccgccg cgatggtatc cgcggcgatg gagcggagaa cccccgcgta ttcctcctcg
     2581 gcggcgagga aggaacagtc ctcgccgatg gcctgcccca cggcttccac gtccaccggg
     2641 aatccatcga agcggtcgct gaagctgaac cgccagaacc gcgaggtgaa taccggcacg
     2701 gtgtgaggcg gggacgacac cagctgcagg tagttgatga tccggccgac ctcgctgtac
     2761 agcttcccca tgctgtcggc cttgtcgatg cgcgcaagaa tctcgttgaa atgctcgtga
     2821 aagcgcgtgt cgatcgccca catcaccgag tgctccgcct cggcggcccg gtcgtagtag
     2881 cgccagttga acatgcgggc gaacgcgttc cgatccgcga ggcccacgtt cgtcttggcg
     2941 aggtagcgga tctgcagcgg cgtcagcggc gggacctcgg tgcccgcggt gcagcggttg
     3001 aagtgctttg ccgccagaaa ggtcaggtcc tgatgaatcc ggccatcgta cccgagcgca
     3061 tgggccgaca ggcaccagag cgcgattacc aggcaacgcc taaaggcaac catgcgcttc
     3121 cagacgcacc acgcgctcgt cgctgtcgag ctcgtcgagc agctcccgga tcgtcaaccc
     3181 gatttccggc cagaccacgt ccggtgccac ctgcgccgcc ggcgcgagca cgaagaggcg
     3241 atcggcggcg cgcgggtgcg gcaggacgaa gtcggccgtg tcccgcgtct cggcgtcgaa
     3301 cagcagcagg tcgagatcca gctcccgggg ggcgttacgc aagcgctgcc cggtccggcc
     3361 atattcggcc tcgagcgctt tgagatcagc aagcaggctc tccggcgtca acccgggggc
     3421 ggcacgaaag gagaccgccg cgttgatgaa gtcttccgac cccggtgggc aatccaccgg
     3481 cgacgtgcgc cacagatcgg agcagcgcgt gtcgccgtcg gcaaacccac gaagcctgcc
     3541 catggcggcg cggacgatac cgacgctgtc gccccgattg gatccaagac caaccaatac
     3601 catcgttttt ccggtgtcct agcaggcatc ccccagggcc aatactaacc tctgctatcc
     3661 cccggtagta agggagatcg ggcgccggga ccggcggcag atggtagtaa tcggaccatg
     3721 cgtttggcac actcaaacgt cggacacgta cagggagagg cccatgaaag ccgccgtttt
     3781 gcgcgagatc aacaagccgc tggaaatcga agatatccag cacggcgacc cgggcccacg
     3841 cgaggtgctg gtgcgcacgg tggcggccgg cgtgtgccat tcggatctgc acttccagaa
     3901 cggctcgtat ccatatcccc tgccggccgt gctcggccat gagtccgccg gcatcgtcga
     3961 ggccgtgggc agcgacgtca actacgtcaa gcccggggat catgtgataa cgtgtctttc
     4021 ggcgttctgc ggtcactgcg agtactgcct gaccggccac atgtcgctgt gccaggagcc
     4081 cgagctgcag cggcccatgg accagccgca gcgcctcaac aagggcgacg agaacatctg
     4141 gcagttcctg aacctgtcct cgttcgccga gtacatgctc gtccacgagc acgccattgc
     4201 gaaaatccgg gaggacatgc ctctggatcg ggccgccctc atcggctgtg gcgtgacgac
     4261 gggcgtcggg gccgtcattc acaccgccaa ggtggagccg ggcagcacgg tcgccgtgat
     4321 cggctgtggc ggcatcgggc tctccgccat caacggggcc gccatcgccg gcgccgcccg
     4381 gatcattgcc atcgacaccg tggaaagcaa gctcgatctg gcgcgcaaat tcggcgccac
     4441 cgacgtcgtg aatgccaggg agagcgacgt catcgaggcg gtgagagaga tgaccggtgg
     4501 cggcgtgcac tactccttcg aggccattgg tctcaagcag acggccgagc agtctttcaa
     4561 gatcctgcag cgcggcggca ccgcgaccgt catcggcatg attcccgtgg gcacgaacat
     4621 cgagctgcat ggacccgagt tcctcatgga gcgcaagatg caggggtcga acatggggtc
     4681 caaccgattc cgcgtggaca tgccgcgatt cgtggagttc tacctgcagg gccgcctgca
     4741 tctggacgat atgatctccc gccggatccg cctggaagac gtcaacgacg gcctggccgc
     4801 tctggagacc ggagagctgg cgcgcagcgt catcatgttc gactaaacgc ccggccgggc
     4861 cgccggtcag cgaatggcga cgtacccgaa ctggcctgag cggtacaccc ggagcagcag
     4921 ctgaccgtcg tcgagcctgg cgacttccct cagctctgcg aggttgacga tggcccgacg
     4981 gttggcggcc acgagaatgt ctccaggtcg cagcccgaac cggtaagcgt cgctgtccgg
     5041 gtcgatgtcg agcaccagca cgccggcacc catgcccggt tcgtcctggt tgcggaagtt
     5101 ctgcaactgc gttcccgcca accggcgatc gatgcggcgg ccgcgcacct tctccagctc
     5161 gtcctcgccg atcttgaggg tgacctgctg cggctggccg tcgcgaagca cctcgatccg
     5221 gacctggtcc ccgacgaacg tcaccgccgc ctggctgtgg aaatcggcga tcttgccgat
     5281 ctcccgatcg cccacgcgag tgatgatgtc accggcctga agcccgccgg actccgcggc
     5341 gctgtccggc tcgacctcaa ccacgaccac accttcgcgg cggtcgacgc cgaacgcttc
     5401 cgcgagatcc acgttcagcc cctgaacggc tagaccgagg tagccgcggc gcacctcgcc
     5461 gtgctcgatc agctgatcca tgatcgcccg gaccatgttc gagggaatgg cgaagccgat
     5521 gccgacgttt ccgccgctcg gcgccaggat ggcggtgttt atccccacca gacgcccggc
     5581 caggtccacg agggcgccgc cggaattgcc cgggttgatg gacgcatcgg tctggatgaa
     5641 gtcctcgtac ccctcgatgc ccaggccgct gcgacccagc gcgctgatga tcccgctcgt
     5701 caccgtctgc cgcagcccga agggattgcc gatggccacg acgaagtcgc ccacccgcag
     5761 ttcgccggag tccgcgaagc cgatctgctg caggtcgtcc gcctccacct ttagcaccgc
     5821 cagatcgacc tggggatcgc tgccgacgag ctcggccgtc atcgaacggc cgtcggccag
     5881 cgtcaccgag atctcatcgg cccgttcgac cacgtgattg ttggtcacga tgaagccctt
     5941 gccggcatcg atcaccacgc cagagcccgc actctgggtt cgccggtaac ggcgctggtc
     6001 agggacgttg aagaaccggc ggaagaacgg atcctccaac agcgggttgc ggacctgtac
     6061 cgtggtgtag gtggcaatgt tgaccacggc gggcgtggcc tggtcgagca tgggcgcaag
     6121 gctgggaagc tgctcgccgc ccggcagccc cagcggcaaa gccgcagcgg ccgtcaagct
     6181 gcccagcagc agccacccga aaaccgcgca cttcagagtg tgcctcaatt gaccgttcct
     6241 caccttggtt ggtagcaccg atgttggaca gcgcccggtt gccggcagtt ccaccaggac
     6301 ggggcggccg gcagggactc agtgtacact cactgctcgc attcggggga gcacgaacgt
     6361 gccgatcaaa aatgtcttcg aagccatcca gcgcgagaag gcgaacatag agaacttgag
     6421 cgtcgaggag ctcaagcagg ctctggcaga cgaccccgac ctgctgctgc tcgacattcg
     6481 cgagatccag gagcgcgtgg accttggcac catacccggt tccgttcacg tccccagggg
     6541 catgctggag ttctgggccg acccggctag cccttacttt cgggactatt tccaggaaga
     6601 ccggcgcacc gtggtgttct gcgccggcgg cggccgctcc gtgctggccg tcagagccct
     6661 gcaggacatg ggctttaccg acgtggccca tctcgagccc ggcttcggcg gctggaaaaa
     6721 agccgccgag cccatcgaag acgtggcttc cagcagccgc tgggtacggc gggccgacga
     6781 cgactgaagc cgtgccgctt gcgggaccat cacctcggga tggtcaccgg cgagcgctcg
     6841 tgccagaatc gacaacctct cggcgggact agctcatctg gtagagcgac agcttcccaa
     6901 gctgtaggcg acgggttcga gtcccgtgtc ccgctccatc ttcaccccgg gaattccgag
     6961 cgcagatcgc tgccaccctg ctcggtcgcg accagcgtcg ccggccacat cccccggacg
     7021 gagttgagca gccagatgcg ctccgcctcg acgagcaggc taaccggaac gcgcgcttct
     7081 tcgaggattt tgtcggcgac gagcacctga cgttcgatcc cgggaagcag gccgcagccg
     7141 agctcgggcg tcagcaggcg gccgtcgacc tccacggcca cgttggcaat ggtcgactcc
     7201 gtcacctgcc cgtcgccgtt gaccagcagc acatcgtcca gatccggcag gacgctgcgg
     7261 gccgcgtcga gcgccttgtc atacaccgaa cgttgcgtcg tcttgtggta cacgaactcg
     7321 ctctgcaaat cctccatcgg ttcggatagg gccacacggt agggttcggg caaaggtttc
     7381 agcggcgaag cctctagcct tacgtgaccc gaacggtcgg cgagcaggcg gacgcgcatg
     7441 ggctcgcgat acgccgacgc ggccgcttcg agcaaggctt gcgcctttcc ggtgctcagc
     7501 gaccagcaga aatagtcagc cgcgctgtgc agcctctcga gatgctcctc gagacggcga
     7561 aacccgtcac gggggtgcca ggccagggtt tcgagcagct cgaattcccg gcgcccaccg
     7621 gtgacgatgc gcgctttcgc gaagcactcc cgatactcgt cttcggctcg ggagtcccag
     7681 acgatgccgc cacccacacc gtactccgcc tcggacgtcg tcttgtcgat ggcggcggtg
     7741 cgaatagcca cgttgaactg gcagcgcccc tcaggacgca cgaagccaat ggtgcccgtg
     7801 tagatcccgc gcggctcatg ctcgagctcg cgaatgatct ccatggttct gcgtttgggc
     7861 gctccggtga tggaggccgc cgggaacagg gcgcccatga cgtccggcag gctggcggtc
     7921 gatcgtgctc ttaccgtgct ggtcatctgc cagacggtgg ggtaccgctc cagggcaaag
     7981 aggtcgtcgg caaccaccga tccgatcctg gcaatgcggc caaggtcgtt gcggaccata
     8041 tcggtgatca tcaggttttc ggcacgattc tttgccgatg cggccagcca gccggcctga
     8101 tcccgatccg tttggtcccg cagaccgcgc ggcgcggtgc ccttcattgg ccgggaaacg
     8161 agctcgtccc cgtcgcgggc gaagaacagc tcgtgggatg cgctggcaac ggcgaacccg
     8221 ggcgtgtcga tgaaagcagc caggccaggg ccctgagagc tcaccatgtt cgcgaacaag
     8281 gcggcggggg aatcgttcag acgagcgcgc aggcgaaacg agtagttcac ctgataggtg
     8341 tcaccagccg cgatgtagtc tcgaatccgg gcgatggccg aatcgtagtc cttgcgagtg
     8401 acggacgccg accatccgcc aaggcagtcg cccggggcaa gaggaaggtc ggcgctcggc
     8461 agaacccgcc ggaacagacc gaaccatgcg agcggaagga gccggtgcgg cctgtgcgtc
     8521 accagctcgc gcgcaattcc cggagcggct tcataggtca gaaatcccgc cgcgaagagg
     8581 ccgtcgccca ccgtgcggct ctcgatctcg gcgacgacgt caaggacctg atcctcgcgg
     8641 tccgcgatca gcagctcaac gggatcgcaa aagcgcaacc attgttgcgc gacggcatcg
     8701 tagactatga cgtctgtcga catgggcttg cggatcaggg tggccgggta tgctaaccgc
     8761 ggtcagctgc gagactcaat gcgccattcc cggacaggcg gcccctaccc accctgggca
     8821 gatgcggtag gcaccgaaga agggtcagtc gaagtcgagc agatccgcgg agatcacctc
     8881 taccgctgcc cgttctccca gcatgaacgc ggccaccgcc cgacggaagt agtcccgggg
     8941 ctcttctaga aacgccgcat gatccgcccc gggtatgctc acccaggcct tgtccgccgt
     9001 ggccagcccc gcaaaaaagc gcgagagaac cttggggtcc gtgagcgggt cgtgctcagc
     9061 gtccacgagc agcgttggca cggtgacgcg ggcggcgtcc agagccagcc actccggata
     9121 ggcgcgccag tcgacgcgca ccggatcgtg cttgagcgcg ctctccacga acgcatcgat
     9181 cgtttcccgg cgggcagccc cgggggcaat gaaatccgac ctggcatccg ctgcggaatt
     9241 ggcgatccgc gccggttcgg tggtcagtcc tggtgggttc accgccgagt cctgccgcaa
     9301 ggggtagccg aaaacaatga gccccgagat ccgcccgggc tgctgctgag cggcgagttg
     9361 ggccaccatg gcccctcggg agtatccgaa caggtacggc ccggggaggc cggggttgcg
     9421 ctgggcaatc cattccagca ccgcgatgac gtccgcagcg gcccgggacg gggtgagcca
     9481 tccggaggca tcccggggcg tgtccccgta gccgcgcatg tccagcgcgt acgtggcaat
     9541 accctcctcg accagaccgt ccatgagaga aagatcctcg ccgggcacgt tcagatcgaa
     9601 gttgggccgc gcgctccacg tgcgcccgtg aacgagcagc acgacgcgct tggcgctggc
     9661 cggcgctttc cgccataccg caagcggcac gccgtcgacg atcacgtggt gggccgagag
     9721 cggtcgcgcc ggctcgctcg agatcgccgg cagcgaaaga accgccagca gcgaccccag
     9781 gataagccac gtcagggctg ggggccccgg cgtcaacttg ctactcatgg tctgcgtcca
     9841 aactccgggt cattcgacga cgttgcacgc cccccggacc gcgcaatcct tcgcgactat
     9901 agattcaacg ttctggaacg tctaatgttc agagcatcaa aacaacgcgg ttcacggggg
     9961 aaacgagatg gacgtctcgg gcaagatcgt cgtcgtcacc ggcggcgcca gcggtatcgg
    10021 gcgtgccatg gcgcagcgct ttcatgccga gggcgcaggc aaggtgatcg tcgccgacct
    10081 cgacggcgac ggcgcccgcc gcgtggccga cgaaatagat ggttcgtcca tgcaggtaga
    10141 cgtcggcagg gaagcagacc tcgtcgcgct gatcgagcac gctgaatccg agttcgggcc
    10201 gatcgatctg ttctgttcca acgcgggaat cgggatcggc aaaggtctcg acgagcccga
    10261 cgaggtgtgg gacaccatct ggaaggtcaa caccatgtcc cacgtctacg ccgcccgcca
    10321 catggtgccg cgcatgaagg cgcgcggagg cggttatctg ctcaataccg cctcggccgc
    10381 gggcctgctg acacagatcg gctcggtcac ctacgcggtg accaagcacg ccgcggttgc
    10441 gctggccgag tggatcgcga tcactcacgc tcgcgacggc ataaaagtct ccgtgctctg
    10501 cccccaggcc gtgcgcaccc cgatgacggc cgatggcccg ggcgtggctg gcgtggacgg
    10561 catgatcgaa cccgaggagg ccgccgaatg cgttatcgaa gcgctgcgcg aagagcgctt
    10621 cctggtgctg ccgcatccgg aggtcgccac ctacatccag cgcaagacgt ccgattacga
    10681 ccgctggctg aacggcatgc gccgccttca ggatctgtac gtcgccgaag aaggcgagtg
    10741 agcgctgccg ccgacgccgc cggctgagcc agccgccccg gtacgaagct tcagctcagc
    10801 aggtccgcca cggcccgctt cgccatcacc ttgaccaggt gcgcccggta ctcggcggag
    10861 gcgtgcaggt cgttgttgaa tcccgcatcc gggacctcga cgccgtccag cgccgacggg
    10921 tcgagattgc ggctgagcaa ctcctcgata gcagtggcgc gaaaggcgca gggacccgca
    10981 ccggtcaccc ccacgcggac cttgccgccg aagtcggcca ccagcacgcc caccacggcg
    11041 tagcgggaag cagggttcgg gaacttgcgg taggcggccc gggtcggcac gggaaagtcg
    11101 atccgcgtaa tcagctctcc ggggttgagg gccgtctcga agagatcttt gaagtagtcg
    11161 tccgcttcga tgctgcgcgt ctgagtatgc accgtggcgc ccagaccgat cagcgcagcg
    11221 gggtaatcgg cggccgggtc gctgttggcg acggatcccc ccagcgtgcc tcggttgcgc
    11281 acctggccgt cgccgatctg gccagccagg aaagccagca caggaagggt cgcgcgaatc
    11341 tccggcgagc tcgccaccgc atcgtgaacg gtgaacgcgc cgatgctgac ccggtcggcg
    11401 ctcacctcga taccgctgag cgcttcgata cccgacaggt cgatgacgtc ggtaggcgcg
    11461 gccagccgct gcttcatcgt cgggatgagc gtctgccccc cggccatgta gatcccgtcg
    11521 tccatagatt cgaacagccc gacggcttcc tctaccgacg ccgggcggtg gtagttgaac
    11581 tgatacatgg cttctcctag gcccgcagcg cccgccacac cttctcggcc gtggctggca
    11641 tggaaatgtc gttgacgccg agggcgtcgg tgacggcgtt catgatcgcc gccggggagc
    11701 cgatggcgcc cgcttccccg cagcccttga cgccgagagg gttgtgggtg cacggcgtca
    11761 ccgtggtgtc gaccacgaag tccggcacgt cgtccgcgcg cggcatgcag tagtccatgt
    11821 agctgcctgt ctgcagctgg ccgtagtcgt cgtacacgcc gtgctccagc agcgcctgtc
    11881 cggcaccctg agcaagccct ccgtgaacct gcccgtcgac gatcatcgga ttgatgacgt
    11941 gcccgaagtc gtcgacggcg acgaagttga cgagccggac gacgcccgtc tcctcttcga
    12001 tctccacctc cgcaatgtgc gttcccgccg ggtaagtgaa gttctcggga tcgtagaaag
    12061 ccttctcgga aagtcccggc tcgagatcct cggggtaatt ggccggcacg tacgccgcaa
    12121 aggcaacctc cccgaaggac agcgactggt tgctgtccct gacgacgaaa ttgccgtcgg
    12181 cgaactccac gtcgctctcc tgggcttcga gcatgtgcgc ggcgatgcgt ttacccttgg
    12241 caatgacctt gtccgtggcc gcgatcaacg ccgaaccacc aaccgccaac gacctcgacc
    12301 cgtaggtacc gaggccgaac tcgacgcggc cggtgtcgcc atgcaccacc tcgacgctct
    12361 caaacggcac gccgagccgg tcagacacca cctgggcaaa ggtggtctcg tgaccctggc
    12421 cgtggctgtg agatccggtg aagacggtga cggaacccgt cgggttcacc ctcacctcgc
    12481 cgctctcgta gagcccgacg cctgcgccga gctggatggc cagctgcgag ggcgcgaggc
    12541 cgcaggcttc gatgtagcac gcaaggccaa tgccacgccg cttgcctgcc gcttcggaag
    12601 cagcgcggcg agccgggaag ccggcgtagt cgacgagcgc aagcgctctg tccagaccgg
    12661 cttcgtagtc gccggtgtcg tagaccagcg ccaccggcgt ctcgtagggg aactgatccg
    12721 gccggatcat gttgcgccgc cgcagctccg ccggatccgt gccgagctcc ctggcggcag
    12781 tctcaacgat ccgctcgacc acataggtgg cctccggacg cccggctccg cggtaggcgt
    12841 ctaccggtgc ggtgttggtg tagatgccct gcacctccac gtagatgttc ggggtccggt
    12901 actggccggc catcagcgtc ccgtagagat aggtcgggac ggaggagccg aacgtggaca
    12961 gataggcgcc gagattcgcg ctggtctgca ctttcagcgc cgtgaagtta ccttccgcgt
    13021 ccaggccaag ctccgcatgg gtgatgtgat cccgaccgtg ggcgtcggcg agaaacgact
    13081 cgctgcgctc cgccgtccac ttcaccggcc gaccgaccct gcgcgatgcc cagatgacgg
    13141 cggtctcctc cgcgtagatg aatatcttgg aaccgaaacc accgcccacg tccggggcga
    13201 tgaccctcag cttgtgctcc ggtgcgacct gaacgaaggc ggacaacacc agccgctcga
    13261 ggtgggggtt ctggctggtg gtgtagagcg tgtaatcgtc gccggccgga tcgtatacgc
    13321 ccagagcggc gcgaggctcc atggcgttag gtatcaggcg gttgttggtc agatcgatcc
    13381 gggtgacgtg ggcggctccg gccagcgccg cttccgtggc ctcccggtcg ccgatctccc
    13441 aatcgtaggc ccggttcccg ggtacgccct cgtgaatctg gctggaatcg acggcggtcg
    13501 ccaggtcgac taccgcgtcc agctcctcga aatccgccat gacgcactca gcagcctgcc
    13561 tggcggcggc cagtgactcc gcgatcacca atccgtaggg ttcgcccacg tagttggcga
    13621 cttcggtggc cagcggcggg tgcggagcgg agatcatgtc cgagccgtcc ttctgcttga
    13681 cgagccaccc gcagggaagg tcacccaggc cgtcggctgc catatcccgg ccggtgagca
    13741 cggcgatcac gccctcagtg gcaagcgctt cgctcacgtc gatctcgccg atcctggcgc
    13801 gcgcgtaggg gctgcgcagg aatacggcat aggtctggcc cggctgcgtc aggtcgtccg
    13861 taaagcgccc gctgccggtt atgaaacgga cgtcctcgcg acgcctgacc gacgctccga
    13921 tccccgttgc cgctgcttca ctcatctcga atcctcacat cttctcggct gcttgcagca
    13981 cggcacgcac gatgttgtgg taacccgtgc accggcagat gttgccttcc aggccttccc
    14041 ggaccgcctg gttatccagg tcgtccgact gattcaccag atcgatggcg ctcatgatca
    14101 tccccggcgt gcagaacccg cactgaagcg cgtggcagtc gcggaacgcc tcctgcatgg
    14161 ggtgcaggtc gtctggcgcg ccgattccct cgatggtcac cacctcggcg ccttcggcgt
    14221 gtgcggcgag caccgtgcac gacttgaccg agcggccatt gagatgcacg gtgcacgcgc
    14281 cacactggct cgtatcgcag ccgacgtggg tgcccgtgag cctgaggtgc tcccgcagga
    14341 ggtccaccag gagcgtgttg tccggcacct cgagttcgcg gtcggtacca ttgacgttca
    14401 gcgcgacttt catattcgaa tcctagactc tttgattcac gacgacggcc gctcagacca
    14461 tgagcagcag tatgatcacc acaatggccg caacggccac ccatatcttc cagcgcccgc
    14521 cggcaacagg cgcctctgcc acagccgcca gagcctcggg ctccgcaccg cccacctcgc
    14581 gtccgaactc ggcaaagaaa tcgtctgcca tctttcgggc tgccgcgtcg atcagccggg
    14641 atcccacctg agcgagcttg ccaccgacgt tggcgctcac ctgatagcgg agcaacgtgc
    14701 tggcaccgtc ttctaagagg ctgaccttcg ccgtcccttt cccgaacccg gcggcacctc
    14761 ccttgacgtt ggccgtcaac gtgtagctgg agggcgcgtc gatctcgctg agcatgagct
    14821 gcgcctgaaa ggtagcgctg accggaccca ccttggcctt gaccgaggca tcgaactgct
    14881 catccgacgt ccgctccatg gacaggcagc cctggatgca gcgcccgagc acctcggggt
    14941 cgttcaggcc ttcccatacc attgcccgtg cggcggaaat gcgatattcg ccgaactgct
    15001 ccactcggaa cctcctccct cgagcgccta atttcgcacg attgccttat cccggcaacg
    15061 cgttcagtcc atgggctctc ccagggcccg cgcgcggtcg agccggttgg cctcctcggc
    15121 tccgaggtac ctcatggcgc cggcgaggaa caggaggctg acgcccagca tgccgcagcc
    15181 ccagagaatc gccctgccca gcgcctggcc atcatcaagg cccgacgcca gatagatgtc
    15241 gctcacctgg ccgatcagat agggcccgag ggcgagcccg atgaaggtaa gcatcaggat
    15301 gtagtaggcg gacgccaccg ccctcattcg cggcaagaca agatcattca ccgtgctcgc
    15361 tgcggcacca agccacatgg gcgagaacat gctgaacaca aacgcgcaga aataggccac
    15421 ccagacgttg tcggtcagca cgaaccagat ggccgcgggt gcggcgagaa agggcgaagc
    15481 cacgccaatg tatatgcggc cgttcaccga acgcgcccgc agccggtccg ccacgatgcc
    15541 gcccagcgtg acgcctatcc agccgcccag ggcggaaccc aagcccagca gcgtgccggc
    15601 ctcggcgaga ttgacgccat gggcgcgaag cagatagggc gggctccaga aacccacgcc
    15661 gtaggtcacg aaagccaggc aggggaagcc cagggtcagc agcaccatgg cacgggagcc
    15721 gaagatcatg ccgaacgcca caggatccga aagcttcagg ccctgggccc acgagaccac
    15781 cgcgtagacg ccgattccca gagcgatcca ttgattcggc gtgcccgtcg ccaggttcgc
    15841 accgtagccg acggccgcga tgagcagggc gagtccgaga ttgacggcga cggtccgggc
    15901 gccgccgctg acccatagcg agaccacggt gaacggggga agcacggtga cgagcgaccg
    15961 ggccgtttcg cggaaaggat gcgggtgcgt cggcaccaca agcccctcgc tgaccccgcg
    16021 ctgcggctcg cgcagcgtcc agacccagat ggccatgagc aggccgggta ggcctaccgc
    16081 aaagaaggcc gcctgccagg ctttgaggcc gaacggcgcc aggacgggat ccggaaaggc
    16141 gtcattccag gaatcgacga tgatcccacc gaggaagacg ccgataccgg cgccgatgta
    16201 gaccccactg gaatagatgg caagaacggt ggcccgcacg cgcggcgaga agtaatcgga
    16261 gagcatggag aaagcggccg gcgacgcgct cgcctcaccc acgccgaccc cgaagcggaa
    16321 agtggcaagg gaagcaaaat tccgggcggt ccccgacagc gccgtcatca gactccagaa
    16381 gccgagaccg atggcgatga gatccttgcg gatccaggtg tccgccagcc gagccagggg
    16441 aatgccgaac acggcgtaga agacggcaaa agcggttccg taaaggaaac cgatgtccgc
    16501 atcggatatg cccaggtcgg ccttaatttc ctcggcgagg atggacagga tctgccggtc
    16561 gatgaagttg aagacgtaaa cgatgatgag aacgatgaga acgtagttcg catacctacc
    16621 gcccacccca tcggccgatt ccccggaaga atcgctcggt tcgttctgct gcacggctct
    16681 cccctcgacc cggtggcatc cccttgtgcg ctgggcagcc tagcaaacca cgaagcgatg
    16741 tccatggtta ggcggcgcgg gcgccttcaa tcgcccagcg gcgcgccgtc ccagcgttcc
    16801 agcatcgcga actcgtccac cggcttgcgg ctgagccggg tcagctgctt gaccggccgc
    16861 ccgatcggga ggagggccgc cacggcgagg tggtcgggta ttccgagaag acgcctgatc
    16921 tccccctcgc gcccggcgat gaaagtggtc aacgtaccgc cgaatccctc gttccttgcg
    16981 gccaggagga cgttccagac gaacgggtag atggaggcgc cggaaatcac cccgacccgg
    17041 tcgagctgcg cgtcgaagga cgccaccacg ctcagatcca cgagaatcag cagcagcacc
    17101 ggcgcggcaa gaacctgccg aatgaaggct tccggcggct cggtagcggc gatctcttca
    17161 gccgttaggc gggtctcgtt gatggtgttc cacggcgctt cgcccgcctg aacctgtgcg
    17221 acgtagcgct gaaacgtggg catgatcatg ggcagcatgg cttcccgggt ctccgcttcg
    17281 cggatcacga tcacccgcca accctgacga ttacccccgc tgggggcaaa ccgcgcatta
    17341 tctaatatat tgcgtatatc cccgtctgtg accggttcgt ccaggaattc ccgggccgcg
    17401 aaggtcgttc tcatcacgtc gtagagttcc acctgatctc ccaatttata acagttgctt
    17461 gacagcgcac tttgagaaac aataccggat actgcgtacc tcggaaacgg gccgcatgaa
    17521 ttgtgttgcg attgattgag gattgacatg gaagatcaac ccaacgaagt gctgatgcgc
    17581 ggcttgctgc gcgcctacta ttggatggat gagagcctcc agaacgggct gcagaaagca
    17641 ggctacctgc ctcgcacccg cactcagacg atgattctca tcaacatatc caacggcgtg
    17701 accagagctg cggagctggc acgggtgctg ggcgtgagcc ggcaggccat ccagcagcag
    17761 atcaacgaac tggagcgtga cgacctcgtc acccagcttc cagatccgga cgaccggcgc
    17821 gccaaccgaa tcgtcttcag cgagcgcggt tcgcagctga taaacgctgc actggcaaca
    17881 ctgcgccaat cagagcaggc cctggcgata cggctgggct acgagacggt gaaccagctc
    17941 cgacgcgctc tgatggcaga ttggggcccc gtgatcggag agcggcgcgc tcccggacgc
    18001 aagcgctcca tttcgaacca gaacgactga ccggcgcgcc gccttccggc ggcgctctcg
    18061 ctccccccgg acctggcgtc gctgaacgcc gggcgggtgc gactcccccc caacatcgtc
    18121 gccgagccga tcaccttcgc atgaatgtgc ccgtgccgac cacggccgcc gagccgaatc
    18181 gatagggctg ccagtggagc gacttcgagc cgcgttcgct ctcgtcgaag tcgttggcat
    18241 gaatgccgac gcgaacgacc tgccactccc tgccctgctg gcgactgagc agttccccag
    18301 ggatggcgat ctcggctgcg tatccgccgg acgttcgcac caccgcgctt gcaatggcgt
    18361 cgcgcacgcc atcgagcatc tccagcaccg gtgccgggcg cccgggggcc agggtcagca
    18421 gctctatgac catgcgcgac gtggcaccgc tggccatggc ctctactaga ggctggttcg
    18481 cagagcggtc cgggtcggga cgggcatcca ccgagacgat gagcgcgtcc tgatccatcg
    18541 cggtcagctc accggaggca acgatggagt cgtcgattac ctcggcagca aggtaaaggt
    18601 tctcgtcgtc gtagccgatc ccgaagcgaa acgagatgtc cgccggatca gtctgcgggc
    18661 tggcaatgtc accctggcgc gtcaccgagt acggcagatg ccattcctcg aggtctccgt
    18721 ccaggcgaac cagacggctg atgcgctcca gcggaaagcg gcgctgggga agcagcgcgg
    18781 tctcgatctc tacgaatacc ggccgatcga gcagcatcga ccgcaacgac caggtgaccc
    18841 tcgccggggg caggctctcg aaggcgagcg cgtgcgtgga tttcagattc agcgcgaccc
    18901 gccgctcccc tccgggcggc accgagattg cctggggagt cgcctgggcg cgcagaacgt
    18961 cgtttcccag gacgatgggc gtcgcctcga ggggagccga ccccgagttg acgatccggt
    19021 agcgaaccgc gcccgaggtg aacaggtctc cgtcgagata ctcggtttcg acctgcaccg
    19081 cgtccgacag ctgcctcaac gcacgcagcg agtcctctgt ggtgacgtcc tcgggttgaa
    19141 tcccatcgag catcagattc gcgatggtgg gtccgcgcga ggacatgtgc accatcgcca
    19201 catgatcaaa ctcgccgtaa agagcgcccc ggagcgggct ggccccgccg gtggtcgcca
    19261 gcgtgatgta cttgcgatcg tggcgtaacg atcgctggta gcggtgcgcg tgaccggcaa
    19321 acaccgtata cgggcgttca ccgaggagcg cttcgacctt tagccagtcg gggtgaacct
    19381 cggcgacgtc ccacagcggc tgatgggtga tgacgatcgt ccagcggacg tcggcatgcc
    19441 tggagagcac gcgctcgatg taactcatct gctcttcctg ggtccagggc cccggcacgg
    19501 acacccacgg attcgtcacc atgccgaaca gctcggagtt gacgaccagg aacagcacgt
    19561 ccttgtagag gaagtgatag aaggacggtc cgaaacgggc gcgccaggcc tcggacatga
    19621 ccgcgttgga catgtcgtga ttgcccggcg cgtagaagaa gggcgcatcg agctggtcga
    19681 caaaggcctc gacttcatcc cactcccccg tgagccgggc cggatcgtcc gtgtagcctt
    19741 caatgagatc tcccacgctg accacgaatg ccggctgcag cagattgacc ttgggcatgg
    19801 cggactcgaa cacgcctggc cgatgaccgc gcgtgcggtc ggccaccacg acaaaggtga
    19861 aatcctcttc cgcatcgttt gcctggagtc ccgtccacgg ggtggctgag gattcaacgt
    19921 gagcttcgat gcggctcgcg tggtcggcag ccagggtgac tcccgaggag aacaacgcga
    19981 cgagcacaac caggcccctc gcgggccagc tcgccctgac cggcgggcgg ctcgcggacg
    20041 gccgtcctgt cttctgccga cgcgaatctt tcaagccagt cggccctctg ccaccagttg
    20101 caccttgccg ccgacgcgga tctcgtctcc cacgtcgaga tagagccgcg agggccgttt
    20161 catctccacg ccctgatcga cgacgacgcg cccgccaggg cccggtcgaa ctccccgccc
    20221 agccgcacgg gcgggggcgt gaaatagcgg gcgcgggcgt ggccgctgcc gctttccagg
    20281 accaaggcgg tctccgagaa attcatctcc cgggcaatgt cctgcatctc tgcggtggag
    20341 aggccgctcg catctcgcac caccgccagc tgattaccgg cgtagcggcg ctcggcgaac
    20401 acgtcgacga tggtgagctg cattcgagaa cccaaggcgg cacgtttcgc catcgagact
    20461 agcacccgct ctctgcattt cccaacctcc gcccatcagg ccgcgtgatg aacttgcgct
    20521 aaactcgctc atcagcaccg gagggcttcc cgatgacagg cacagtcgag ggcatatatc
    20581 tggccgcaaa gcacggcgat tcccagcggg ccgtgcccgc ggctcgactg accataggcg
    20641 tcggcctcga gggtgatcgt cacgccaact cgaaaggcgg ggtggtcacc ctgatagagg
    20701 ccgaggcggt agaacggttc atcgccgaga cgggcctgga catcacggcg gcggacaccg
    20761 gtcgaaacat cgccacccgc ggcatcggcc tgaacgacct ggtgggcagg cagtttcgcg
    20821 tcggcggggt gctgatggaa ggcttcgagc tatgcgagcc ctgcgccacc ctgggcgcgc
    20881 ggctggcgag tcgggacgtg agcgcggcgg ccatcgtcgc tgcctttacg cacagcgggg
    20941 gcattcgcgc cttcgttcgg ggaaccggca ccgtggcacc gggaagcgcc gtcgagccgg
    21001 cctgatcgca tctctcacac tcgcagcggt tcggcaacct gatccgtgtg accctcgatg
    21061 tcgtaggaca tgatcaccag gtcccgcggg gttccgctac ggtcctccac gtagtccgcc
    21121 agcagcgcct cgggcacaaa gcccagccga cgaaaggccg cctgggcacc ggactgatcc
    21181 accgtcatgt tcgccatgag ctttcgcaag ccgagcgacc gggccaggtc gaaaatctgc
    21241 gacgtcagcg tccggcccag ccccttggcg cggtatgcgg ggcttacgtt cacccggatc
    21301 tcccccactc tgcgggtcca tcgggcgggg tcgcgatgca cggtcgcgta ccccaccagg
    21361 ccggtgccgt cgtaagccac gatcgaagtc gagtgacccg tctcgaggtt gcggatccac
    21421 tgatcgatca ccgcgggctc cgtcaggtcc acgcgcagaa acatcaggtc ttcctcggca
    21481 agcccccggg caaattcgca aaccgcgtcc cggtcctctc cggtcatcag gcggatctcg
    21541 atctcttcgc cgccgggcag ccgcacctgc ttggggtacc cgtccgcacc gctctgtctg
    21601 gtcatctttc tacaccctcc tcagtcaggc cgccggcgag tcgtaggact tccggcttgc
    21661 cttttccgac cggccgcccc ggctgggcgg ggccggcggc tcgttgaccc ggcgcgcttc
    21721 ctcgagcagc tcctgataca cctgctcgac agcctccacc atcacgtcca gatcgggcat
    21781 caggcgcggc tcgcagacga aattgaagcg catattctgg ttgtagctca cgactgccat
    21841 gccgtacccg agggtgccgg tgagcatcag tatgccgatg atgttgctga tctcgtgacc
    21901 ggccaggtac tgcggcacct gaacgcccgg cacgttggtg caggtgaagt tgaacccgta
    21961 gagcgggagc cgggtgccca ggttgggcac gaccggcaac ggcattcgtc cagcgagggc
    22021 ggttgggtcg aagcgggttc ccaccagcag cgttggcgcc atggcgaccg caggcacgga
    22081 cggcatcgtc tcgctgagga acgtcagcgc ttgcgcctcc tcgtcccgtt tgatgcgttc
    22141 ggtctctcca atcacagcgc gcagcctctc ggcggcgtcc atggaccagg ccggcagcag
    22201 agggaaaatg gcggacacac gattgccgag gctgccggct tcgtcctcgg tgcgcacgct
    22261 caccgggcac atgattctca ggtacttccc ttcggtttcc tcgcggcggc gatccaggta
    22321 gcgggcggcc ccctctgaga ccatggcgag aaccacatca ttgatggtcc caccgaaggc
    22381 ccgtctgatc tctctgaact cggagaacgg gtggctggtc cagcggagcc ggcgcttcgg
    22441 cccgaccagg ccggcgttcc aaggcgccgt gatggcgggc tcagtgaaga agcgcgagaa
    22501 cgcgcgaccg gcattgcgca ggagctcgaa gctcttgcgg tcggtgtgca cgcctgcgag
    22561 cgggttgacc gacagccgct cggcgctctc ccgcaccacc tcgccaacga gatccatggc
    22621 agagggcggc gttgcaggct gccgcggttc tccgggcgga ggatccgggg cgtcccgatc
    22681 aaagtcgaac tggaccatgg tgagctggat gccggaaacc ccgtcgatca tggcgtgatg
    22741 cacctgctga agcatcagcg ttttccccgg cacgccgtcc accacatagg tcttccaaag
    22801 cggtcggttg cggtcgagca gcccctcgtt caccgcgagc aggagatcca ccgcctcctc
    22861 cagggtcgag ccctcgggaa ccgagtgcgg caccacgtga ttggcgagat cgaagtccgg
    22921 gtcgtccacc cacttcgggt gcgccaggtt gaatggtacg aaggccagct tctgcatgta
    22981 acgcgcaatg aggtgcatgc gagcctgaaa gtgactgaac acacgctcga acggcagctc
    23041 gccctccagc acgaagatgg aagcggtgtg catcgggccg ctggccgatt ccgagtacag
    23101 aaacgaggcg tcgtgcgaag tcagtcgcat gggttcatcc gatcaggggc gcacactatg
    23161 cgcggagacg aaccggctcg tcaacgcgac gccgcggctg ggcggcctgg ctaccggcgc
    23221 tgcgctgcct gtaacattcg acgctcggtt tacccaggga gaaggcgatg cgcggtggtc
    23281 aggtgttcat ggagg
//
LOCUS       DBNHKV010000082         5221 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1924932, whole genome shotgun sequence.
ACCESSION   DBNHKV010000082 DBNHKV010000000
VERSION     DBNHKV010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1924932"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="AC16PP_02525"
     CDS             complement(<1..204)
                     /locus_tag="AC16PP_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003250480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HIR6480528.1"
                     /translation="MAALRGVRLVVVTGKGGVGKTSVAAGIASASARSGLRTVLVTLD
                     TGDTRHPLLDVPLEYQPYQAAPRL"
     gene            375..1148
                     /locus_tag="AC16PP_02530"
     CDS             375..1148
                     /locus_tag="AC16PP_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480529.1"
                     /translation="MTTYEVVARNFSQAHENRIHSDEVAQRYGFTGALVPGVAVYGHL
                     THPVVERLGEDWLGRSVSSVRFLKPAYDGDRLHLDCESRGSALLVSCRNDAGTLLAEL
                     HTEQPRDMPEPVTAAVLDEPRRTAERAEISWEAVVENETFEPWHWQITEDGNAQYAAE
                     VADHLPIYRLCAHPHWLLSTANRALTREYAMPAWIHVGSDVRHRALVKVGDTVEVRAT
                     LMEKWRRKGHEFLRLYVAYRRGRELTTEIFHTAIFKVAA"
     gene            1148..1744
                     /gene="purN"
                     /locus_tag="AC16PP_02535"
     CDS             1148..1744
                     /gene="purN"
                     /locus_tag="AC16PP_02535"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR6480530.1"
                     /translation="MRIAALASHGGSILQAVIDAIENGTLPAELVLVVSNNSRSQALA
                     RASRHGIATLHLSAHTHRDPDELDAAIVRSLEEANADWVLLAGYMKRLGPRMLGRFRN
                     RIINTHPALLPKFGGQGFYGQRVHEAVIEAGETESGATVHVVNEAYDSGPILAQVRVP
                     VEKNDTAEVLEERVKRAERKLIVTTLAELAARPAASGY"
     gene            complement(1773..2531)
                     /locus_tag="AC16PP_02540"
     CDS             complement(1773..2531)
                     /locus_tag="AC16PP_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6480531.1"
                     /translation="MASLELMDANQLDYSPIRRAGVIGDVHAEHERLAGALEWLSGQD
                     VDVILCTGDVADGVGCVNTACSLLRDADVLTVAGNHDRWLLQDRVRHVADAHFLADLD
                     DESRDYLTALPRSRTLNTVAGPLLLCHGVVENDLAKVWPGTERSPIERSTEMDDLVAA
                     STHRFVVNGHMHYRVLIDFEGVVLVNAGTIRGPFSGVTVIDFEGDFAAAYERDGNGRA
                     HRVAEHELTRNGERRIWRDTQEFDGSWQPVTLHP"
     gene            2654..3493
                     /gene="surE"
                     /locus_tag="AC16PP_02545"
     CDS             2654..3493
                     /gene="surE"
                     /locus_tag="AC16PP_02545"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014077.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR6480532.1"
                     /translation="MFAFAAQPCTDRPLNILLVNDDGFDTPGIIALHRELKASGHNVR
                     RVAPDRNYSGSSASLTIDLIAAPRIPDEEFTEVYAVSGSPATAVLVGATAIFPPEQPV
                     DLVVSGINEGANLGPATVISGTVGATIAAMKALARPIPGIAVSTNPIAEDEAAPENHR
                     HLKTVAGFTSRLVRRLFCASQPWSKGELALNVNYPPLEPEAVKGVRVAAQGKAPYYSM
                     GFKAAAPDRYAPDFGRAEPSQDIENADTFLFRQGYITIVPIDGDYTAGGRPLPASLED
                     LEP"
     gene            complement(3806..4648)
                     /locus_tag="AC16PP_02550"
     CDS             complement(3806..4648)
                     /locus_tag="AC16PP_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR6480533.1"
                     /translation="MLEYSEARQISDSERVEYLEFALGLAAAAGSAILPFFRAGAGVE
                     NKRTDGGFDPVTEADHAAERVIREQILGRYPEHGIFGEEFGLDAGNGLTWVVDPIDGT
                     RAFMSGLLHWGVLLALFDGERPVLGVMHQPFTAEFFAGDCHNAHYHRGETSQALEVRE
                     CKGLGQAVLGTTSPKLFTSPFERRGLDAIEERVKLSRYGGDCYLYAMLAMGQLDIVMD
                     AGLQPYDVQALVPIIEGAGGVVTTAQGGDASMGGFVVATGSAELHAEVLATIRAGSEL
                     GPGS"
     gene            4769..5155
                     /locus_tag="AC16PP_02555"
     CDS             4769..5155
                     /locus_tag="AC16PP_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR6480534.1"
                     /translation="MTRDVVLPEQDKRSYEAFHFAPAVRASGLLICSGQIGTSADGSI
                     PESPQEEFRNAWAAVGRILAEAGLSYDDIVETTTYHVNLHEHLTSFMAIRDDIISPPW
                     PAWTAIGITELAVPGARVEIRVMAEA"
BASE COUNT         1058 a         1736 c         1536 g          891 t
ORIGIN      
        1 aagccgcggg gccgcctgat agggctgata ctcgagggga acgtcgagca gcggatgcct
       61 ggtatctccg gtgtcgaggg tgacgagaac ggttctgagc cccgacctgg cggaagcgct
      121 cgcgatccca gcagccaccg acgtcttgcc cacgccgccc ttgccagtca cgaccaccag
      181 gcgcacgccg cgcagcgccg ccactgaagc atcaccattc atcgccgcac tataatgcgc
      241 tttcaccacc cggatcgacc ttgactgcgc cgcagccgac ccatcacaga cagatctgcc
      301 taacggacct agagcttcag aggtatgatc agcagacgtt gcggcaacgc atccatcggc
      361 acagagggcc acagatgacc acctacgagg tcgtggcgag aaatttctcc caggctcacg
      421 agaacaggat ccacagcgac gaggtcgcgc aacgctatgg ctttaccggt gccctcgtcc
      481 cgggcgtcgc cgtctacggg cacctgacgc atcccgtggt ggagcgtctg ggtgaggact
      541 ggctcggccg gtcggtgagc tcggtgcggt tcctgaagcc ggcctacgac ggggacagac
      601 ttcatctgga ctgcgaaagc cgagggtccg cgttgctcgt cagctgccgc aatgacgccg
      661 gcaccctgct ggcggagctt cataccgagc aaccgcgaga tatgccggag ccggtcaccg
      721 ccgccgtgct cgacgagccc cgcagaaccg cggaacgtgc ggaaatcagc tgggaggcgg
      781 tggtcgagaa cgaaacgttc gagccgtggc actggcagat caccgaggac ggcaatgcgc
      841 agtacgccgc cgaggttgcc gaccatctgc ccatctatcg cctctgcgct cacccccact
      901 ggcttctcag cactgccaat cgggcactca cgagggagta cgcaatgcca gcatggattc
      961 atgttggctc cgatgtccgg caccgggcgc ttgtcaaggt tggcgatacc gtggaagtca
     1021 gggccacgct catggagaaa tggcgcagga agggccacga gttcctgagg ctgtacgtgg
     1081 cgtaccgcag aggcagggaa ctcaccacgg agatctttca caccgcaata ttcaaggtgg
     1141 cggcctgatg cgcatcgcgg ccctggcatc tcacggcgga tcgattctcc aggcggtcat
     1201 tgacgccatc gagaacggaa cgctgcccgc ggagctggtg ctggtggtca gcaacaacag
     1261 cagatcccag gcgctggcac gcgcgagccg ccacggcatc gcaacccttc acctgagcgc
     1321 ccatacccat cgggatcccg atgagctcga cgccgccatc gtccgttcgc tggaagaagc
     1381 gaacgcagac tgggtccttc tggccggcta catgaagagg ctaggtccgc gcatgctcgg
     1441 tcggttcagg aacaggatca tcaacaccca tcctgcactt cttccaaagt tcggcgggca
     1501 gggattctac ggccaacggg ttcacgaggc cgtgatcgag gcgggtgaga cggaatcggg
     1561 cgccaccgtg cacgtagtga acgaagcata tgactccggg cccattctcg cccaggttcg
     1621 agtgcctgtc gaaaagaacg acacggcgga ggtgctcgag gagcgagtca agcgcgcgga
     1681 gaggaagctc atcgtcacga cgctggccga gttagccgct cgcccggccg cctcgggata
     1741 ctgaggcaac gggacggcct cgcggcgcgc ctctaagggt gcagggtaac cggctgccag
     1801 gaaccgtcaa attcctgcgt gtcacgccag atccttcgct ccccattgcg ggtcagctcg
     1861 tgctccgcaa cgcggtgcgc ccgcccattg ccatcacgct cgtaggccgc cgcgaaatct
     1921 ccttcgaaat cgatcacggt gacgccggag aaaggccctc tgatggtgcc ggcgttgacg
     1981 aggaccacac cctcaaagtc gatcagcacc cggtagtgca tgtgtccgtt gaccacaaac
     2041 cggtgcgtgc tcgcagcaac cagatcgtcc atctccgtgc tacgctcgat cggcgaacgt
     2101 tccgtgccgg gccagacctt cgcgagatcg ttctccacca ccccatgaca caaaaggagc
     2161 ggtccggcga ccgtattcaa agtcctcgaa cgaggcagcg cagtcaggta atcgcggctt
     2221 tcatcgtcca gatcggccag aaagtgggcg tccgcgacgt gccgaacgcg gtcctgcagc
     2281 agccatcgat cgtgattgcc cgcgaccgtc agcacatcag cgtctctgag caggctgcag
     2341 gcggtattga cgcagccgac gccgtcggcg acgtcgccgg tgcacaggat gacgtccacg
     2401 tcctggcccg agagccattc caacgccccc gccagacgct catgctcggc gtgcacgtcg
     2461 cctatcacgc cggcgcggcg aatgggggag tagtccaact ggttggcatc catgagttcg
     2521 agactagcca ccgactcgtg aagtctccgt gaacggatct aaacccgtca ccgagatatg
     2581 gcatgatgat ctgcggccat cctctgtttg catcatgtac cgacgattct tcgcaattgt
     2641 tctgctgtca ccaatgttcg cgttcgcggc ccaaccgtgc acggacaggc cgctgaacat
     2701 actgctcgtc aacgacgacg ggttcgatac gcccgggatc atcgctctgc atcgcgaact
     2761 aaaagccagc gggcacaacg tcagacgggt cgcgccggac cgcaactaca gcggcagcag
     2821 cgcatcgctc accatcgacc tcatcgcggc cccccgaatc ccggacgagg agttcaccga
     2881 agtctatgcg gtctccggga gccctgctac ggcagtgctg gtcggcgcga ccgccatctt
     2941 ccccccggag cagcctgtgg acctggtcgt ttccgggatc aacgagggag caaatctggg
     3001 gccggcaacc gtgatctccg ggaccgtggg cgcaaccatc gccgccatga aggcgctggc
     3061 aagaccgatc ccgggaatcg ccgtgagcac gaatcccatc gccgaagacg aggccgcgcc
     3121 ggaaaaccat cgtcatctga agacggtcgc cgggttcacc agccggttgg tgaggaggct
     3181 cttctgtgct tcgcagccct ggtctaaagg cgagcttgct ctgaacgtca actatcctcc
     3241 gctcgagccc gaagccgtaa agggggtgcg agtcgcggcc cagggaaagg cgccctacta
     3301 ctcaatgggc ttcaaagcgg cagcccccga tcgctacgcc ccggacttcg gccgggcaga
     3361 accgagccag gacatcgaaa acgccgacac ctttctcttc cggcagggct atatcaccat
     3421 cgtgcccatc gacggcgact acactgccgg cggacggcca cttccggcct ccctcgagga
     3481 tctcgagccg taacttctgc cgtgacggtc caggaaccgc agatacgttg accgagcact
     3541 gcgccaaacg gctacaaaac cccgacattg tcgtcacact tcggcaggcg gtcggtggca
     3601 gtctagacgc tcgaggcccc agccggttcg cgccgggccc tcgaggagcg gaccagcagt
     3661 atcggtccgc tctgatcgac ccgtttagtc gcgttttcgg gtcacatcag cccggcgtgc
     3721 cgaaccgacg atcaggacac ggacgtcccc tcgacattcc ggcccgtcga tgacgggccg
     3781 ctgatctgaa ccactcgatt cgaatctagg aaccagggcc gagttcactg cccgcacgga
     3841 tggtcgcgag cacctctgca tgaagctccg cgctgccggt agccaccaca aacccgccca
     3901 tgctggcgtc accgccctga gcagtggtca cgaccccacc agcgccctcg atgatcggca
     3961 ccagagcctg aacgtcataa ggctgcaggc cggcatccat gacgatgtcg agctgaccca
     4021 tggcaagcat cgcatacaga tagcagtccc caccgtagcg gctgagtttc accctttctt
     4081 caatggcgtc gaggccccgc cgctcgaatg gcgacgtgaa aagcttcggc gacgtcgtgc
     4141 cgagaacggc ctgcccgagc cccttgcact cccggacttc gagcgcctgg gaggtttcac
     4201 cccggtggta atgagcgttg tggcaatcgc cggcaaaaaa ctctgcggta aaaggctgat
     4261 gcatgacgcc caggacggga cgctcgccat cgaacagcgc cagcagcacc ccccaatgca
     4321 gaaggccgct catgaacgcc cgagtgccat cgatgggatc aacgacccag gtcagaccgt
     4381 tacccgcgtc taaaccgaat tcttctccga agatcccgtg ttcgggatac cggccgagaa
     4441 tctgctcacg tatcacgcgc tccgcagcgt gatcggcctc cgttaccggg tcgaatccac
     4501 catcggtgcg tttgttctcc acacccgcgc cggcgcgaaa gaacggcaga attgcagagc
     4561 cagccgcggc cgccagcccc agagcgaact ccagatactc gacccgctcc gaatcactga
     4621 tctgccgcgc ctcggaatat tcaagcatcg agtcgttggg ctcccatcca gatatcaaga
     4681 tccacacccg attatgtacg agtgttgtac tgttcgctcc ctcgtcttta tcatcgggcc
     4741 acccccacta tcggtcgagg gtggcgccat gactcgagat gtcgttcttc ccgagcagga
     4801 caagcggagt tacgaggcat tccattttgc cccagccgtc cgggcatcgg gccttctgat
     4861 ctgttccggc cagatcggaa ccagtgcaga cggctcgatt cccgaatcac ctcaggaaga
     4921 atttcgaaac gcctgggcag ccgttggccg aatactggcc gaagccgggc tctcctacga
     4981 cgacatcgtc gaaaccacga cctatcacgt gaaccttcac gagcacctga catcgttcat
     5041 ggcgatacgc gacgacatca tctcgccccc ctggccggcg tggaccgcca tcggaatcac
     5101 ggaactggcc gttcccggcg cgcgagtgga gattagggtg atggcggagg cctaacaatc
     5161 atcaagccag tatttcctcc catgcttaat cctcagccaa attgtgacga tattctcact
     5221 g
//
LOCUS       DBNHKV010000083        16167 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1430131, whole genome shotgun sequence.
ACCESSION   DBNHKV010000083 DBNHKV010000000
VERSION     DBNHKV010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 16167)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16167)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16167
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1430131"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="AC16PP_02560"
     CDS             complement(<1..205)
                     /locus_tag="AC16PP_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480535.1"
                     /translation="MRLLFICLVALAPVAAAHTPVPVHGCAFPERPPDDVDEMTWDRF
                     LEGVERYRACISDFVRSNYAASDA"
     gene            363..1172
                     /locus_tag="AC16PP_02565"
     CDS             363..1172
                     /locus_tag="AC16PP_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007980046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6480536.1"
                     /translation="MTERYQGYQRLEFDYPHPRILRISFNRPERYNALDVVGHNEITY
                     VWREVDADPDIDCVILTGKGKAFSAGGDFEMIEEIIADFDTRAQTWKEARDLVYNIIN
                     CGKPIISAINGPAVGAGLVAGLLADISIVAESVSLTDGHTRLGVAAGDHAAIIWPLLC
                     GMAKAKYYLLTCDRLSGAEAERIGLVSMCVPDEELESRALSVAVQLANGAPNAIRWTK
                     YALNNWLRLAGPTFDASTALEMLGFTSGEAREGVSSLKEKRDPEFKKGSRI"
     gene            complement(1184..1876)
                     /locus_tag="AC16PP_02570"
     CDS             complement(1184..1876)
                     /locus_tag="AC16PP_02570"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007992649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR6480537.1"
                     /translation="MGATSALHAAEPEELLKRRLMAEIRGDAKRTADYTGRSALDERV
                     MAALEAVPRHEFVEAWSPGAAWENVPLPIGHGQTISQPFIVALMTDFVEPHPTHRVLE
                     IGTGSGYQAAVLAGLVGEVYTVEIIPALAQTATERLHRLGYDNVFVRVGDGFYGWPEK
                     GPFDGIVVTAVAPEIPAPLLEQLRPGGRMILPVGEVHGGQNLVLVSKDADGAVATRDV
                     LPVMFVPLTGDH"
     gene            2025..3257
                     /locus_tag="AC16PP_02575"
     CDS             2025..3257
                     /locus_tag="AC16PP_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6480538.1"
                     /translation="MSNETRCCIVGAGPAGMFLALLLARRGVTVTVLELHRDLDRDFR
                     GDTVHASTLEVLDQIGLADRLLALPHAKMRQVTLHTAARTLELVDFSRLKTRFPYVMV
                     MPQVVFLNYLHEQAKSYPGFRCVLGAQVQQLVEVDGRVSGVRYRRDGEEHELRADLVI
                     AADGRFSRLRKLAGLEAESLSPPMDVCWFRLPREASDGHEAGGFFVGQGRMLIALPRS
                     DEWQIGYVFPKGDFKTLRESGIQAFRDSIAATAPWLAGRTDVIHGWERVHLLTVSSDR
                     LPTWHRPGLLCIGDAAHVMSPVGGVGINAAIGDAVEAANVLTRPLLDAVAPGEAALAE
                     VQRRREVPTRIIQGLQARIQDTVVSRALRDEEFHLPLPARLILATPGLRNLPPRIFAQ
                     GIKPLRLEALPRTEHQRT"
     gene            3254..4120
                     /locus_tag="AC16PP_02580"
     CDS             3254..4120
                     /locus_tag="AC16PP_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6480539.1"
                     /translation="MTGLRLGVVGDLHTHWDDIDVAQFNATRYDLLFFTGDLGGGSVE
                     SSLRVARTISRLDVETLVMPGNNDTGDIHQLAAELSHRSGLKRLTGIRQGAGDAIKPI
                     RLCGYSSHRYRTPAADVTLIAARPHSMGGPTLTFADHMSATYGISSLEASAKRLKELV
                     DAAETADLVFLSHNGPTGLGGEPADMWGCDFKPDGGDWGDPDLREAIAHAVATGRRVL
                     AVVAGHMHLKTKCGQERPWRREHDGILYVNAARVPRIYSGSDDVMRHHVSLHIHEQGI
                     EIDELEVPQGLG"
     gene            complement(4117..4605)
                     /locus_tag="AC16PP_02585"
     CDS             complement(4117..4605)
                     /locus_tag="AC16PP_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480540.1"
                     /translation="MNIRTDAIVRLRDHLLSSDLGAAGQVPDEQPGDAVRLRVEPFAE
                     TMFLVMLADAEQAVSEHDALVVAVDMLTDGLMGREAVDALLAEFAERVRREGREDCVA
                     RIGARLSADRDDREMAFTLAAVIALADDRVAVEENEVLDLMRTYYGISDRRMEAILGT
                     LV"
     gene            complement(4602..6029)
                     /locus_tag="AC16PP_02590"
     CDS             complement(4602..6029)
                     /locus_tag="AC16PP_02590"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6480541.1"
                     /translation="MDHAEFEARLGKVLALRIGRVRSLRSAERLSGGASQETYRLTVD
                     TADGEELMAMRRAPGGGLDVEVAPPTLATEALLMRSARAAGVPEPEVYYVLEPEDGLG
                     EGFVMEWLEGEALGARIVRARELDGVRPQLAYECGRVMAKIHAIDLDRAGLRERLETL
                     TPEELLHRSWDRYKDFPTPQPMIDFTARWLLDNMPATFDMALVHNDFRNGNFMVDANG
                     IVAVLDWEVAHIGDPMRDLGWICTNSWRFGRSDRPVGGFGEYADLFRGYEDESGVAVD
                     PERVKFWEVMGSFWWAVGCLGMAEHYRTGPDKTVERPAIGRRSSECQVDCVNLIIPGD
                     VELVAGTDLEDCTDMPRVDELLVSVRDYLRADVMAATSGRTNFLARVAGNSLDIVLRE
                     LATGAEARFREQVRLEALLGRSGDLDALRWELVHRLRSGEMALDDVALTDHLRTTVVN
                     QIAIDQPRYSGFKTALARAGIEVPE"
     gene            complement(6032..7303)
                     /locus_tag="AC16PP_02595"
     CDS             complement(6032..7303)
                     /locus_tag="AC16PP_02595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6480542.1"
                     /translation="MNFEIPEDIAELLDRLDDFIEQVIKPLEQQDDNIRFFDHRREDA
                     RTDWDRGGLPNEEWEQLLFKVKRLADEAGFYRYPFPKEYGGMNGNNLGMAIIREHLAV
                     KGLGLHNDLQNEHSIVGNQVGLLLMLEYGTDEQKAEWVDDLANGVRGFAFGITEPNHG
                     SDATHMETHAERDGDEWVINGEKTWNTGIHKAKYDMVMARTSGKPGDGDGITAFLTPM
                     EADGVKIEEMLWTFNMPTDHGRVSFTNVRVPHVSIFGGEGRGLQVVQHFFNENRIRQA
                     ASSLGAAQFCINESVRYAQERKPFGKALATNQGIQFPLVELQTQCEMLRALIHKTAWE
                     MDTYGAFSVSDKVSMCNYWSNRLCCESADRAMQVHGGLGYSRHKPFEHIYRHHRRYRI
                     TEGAEEIQMRRVAGYMFGFMSQRAPKGVRES"
     gene            7432..7605
                     /locus_tag="AC16PP_02600"
                     /pseudo
     CDS             7432..7605
                     /locus_tag="AC16PP_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            complement(7630..8187)
                     /gene="folE"
                     /locus_tag="AC16PP_02605"
     CDS             complement(7630..8187)
                     /gene="folE"
                     /locus_tag="AC16PP_02605"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIR6480543.1"
                     /translation="MRDRDRLLEAFTTIIEEVDDIDRPGVVDTPRRAADSMLFLTRGY
                     QQTLDDVVNDALFPSDNREMVVVQDIELYSLCEHHLLPFIGRCHVGYIPDGKVLGLSK
                     VARIVDMFARRLQIQEELTFQIANALTEVTGAADVAVIIEARHLCMMMRGVEKQNSLM
                     KTSAMLGAFRENPSTRTEFLNLLKD"
     gene            complement(8206..10128)
                     /gene="thrS"
                     /locus_tag="AC16PP_02610"
     CDS             complement(8206..10128)
                     /gene="thrS"
                     /locus_tag="AC16PP_02610"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017516489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR6480544.1"
                     /translation="MVSITLPDGSQRQFDGSVTGTELARDISKSLAKAAVAVRVDGEL
                     KDIYIPIEQDAAVEVITRDSQAGVDLLRHDAAHVLAEAVKELYPETQVTIGPTIENGF
                     YYDFSRAEPFTPEDLETIEARMREIVARNEEIRREVWDRDEAVAYFQSIGESYKAEII
                     AGIPEGEEVGLYRQGEFLDLCRGPHLESTGRLGGAFKLMSVAGAYWRGDSRNEMLQRI
                     YGTAWATDKDLNAYLVRLEEAEKRDHRRLGREMDLFHLQEEGPGAVFWHPKGWTMFQR
                     MIDYMRRKQIEAGYQEVNTPEILDRSLWERSGHLEKFGENMYLTQTPDDRVYAIKPMN
                     CPGHVQIFRQGIKSYRDLPLYLAEFGKVHRYEPSGALHGLMRVRAFVQDDAHIFCTAE
                     QITEECVKASNLIIEIYRDFGFEDVTVKYADRPERRVGSDEVWDNAESALITALEASG
                     LQWTLNPGEGAFYGPKLEFVLRDAIGRDWQCGTVQVDLNLPERLGATYVAADGSRQYP
                     VMIHRAMFGSLERFCGILIEHYAGHLPLWLAPLQAVVATITDEADEYARSVMAAARAA
                     GLRVEADLRNEKISYKVREHSVTKVPVMLVVGKREAEEGTVAIRRLGGKAQEVLPLDA
                     ALAALGAEALSPLDNH"
     gene            complement(10199..11785)
                     /locus_tag="AC16PP_02615"
     CDS             complement(10199..11785)
                     /locus_tag="AC16PP_02615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR6480545.1"
                     /translation="MDAYDYAIVGAGSAGCVLANRLSADPETRVLLIEAGPGNGNPLL
                     HIPAGWATNFRNPKVDWGFSTEPEPALNGRSIYWPRGKVLGGSSAINGMIYIRGVPLD
                     FDRWAQSGARGWSYEEVLPYFRKAERQQRVADEFHGAEGPLHVQDVRDRRAAMDAFID
                     GMTELGIPRNDDFNGQEQAGCGYYQFNQLRGRRWSTATAYLGPARRRPNLDVMTGVLV
                     RRVCFDGKAATGLEIERARSPATRVAAREVILASGAINSPQLLELSGVGDGERLRGLG
                     IEVVGDLPEVGENLQDHLLTKTVYGTHPEQSINREVQGWRVGVAALKWLMLRRGPLTT
                     GSAPVGGFWYTRDGLDAPDVQVHFASGATLYNEEGRIRAMPVPAMTAVVNQSRPESVG
                     SIHITSADPRQPPAIRPNYLDSELDRDTLVRGLRMIIDIFESTAMRPYVTERLLPEPG
                     VTSDEALLEYIRGDAATTYHPTSTCRIGSVVDENLKVLGVSGLRVVDASVMPFVVSGN
                     TNAATIMIAEKAADLIRAGG"
     gene            complement(11824..12327)
                     /locus_tag="AC16PP_02620"
     CDS             complement(11824..12327)
                     /locus_tag="AC16PP_02620"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6480546.1"
                     /translation="MTLQATFNTSKGAIQVSLMPERAPVTVANFANLARRGFYDGLKF
                     HRVIADFMVQGGCPLGTGTGGPGYRFEDEFDSEVRHDRPGRLSMANAGPGTNGSQFFI
                     THVPTPWLDGNHTIFGEVQGPEDQAVVDSIAQGDVIESVVIEGDLDALLEAHADRIEE
                     WNRALDR"
     gene            12516..13553
                     /locus_tag="AC16PP_02625"
     CDS             12516..13553
                     /locus_tag="AC16PP_02625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6480547.1"
                     /translation="MRLLLVTDAWFPQVNGVVRTLSTTMENLARLGHETEVIAPARFK
                     SVPCPTYPEIRLALCTPGRVGNMIAEFAPDAVHVATEGPLGLAARWWLKRRGIPFTTS
                     FHTMFPQYVRLRFGLPEAWTFAAMRRFHGAATQTMYSTPRLRDMLRGQGFANLVGWVR
                     GVDTELFKPGPAEPLDLPRPLQMYVGRVAVEKSLEDFLSLDTPGTKVIIGDGPQRGEL
                     AARYPQARFLGPRYGDDLVRCYRAADVFVFPSRTDTLGLVMLEAMACGVPVAAFPVQG
                     PLDVVADSGAGVLHDDLGTAIAQAIGIDPEICRARAMEHSWDQSVREFHANLVPIGQT
                     AYHFRGEGETA"
     gene            13550..14770
                     /locus_tag="AC16PP_02630"
     CDS             13550..14770
                     /locus_tag="AC16PP_02630"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6480548.1"
                     /translation="MKILILGGDGFCGWPTALHLSAQGHCVVIVDNLSRRNIDNELEV
                     QSLTPIRPLGERIGTWYELTGREIQFVRMDVATEYDRLLELIRSHRPDAIVHFAEQRA
                     APYSMKSSWHKRYTVNNNLNATNNVLAAIVDSRQDIHLVHLGTMGVYGYSSVGVKLPE
                     GYLNVKMTNDDGEEVEQEILYPANPGSIYHMTKTQDQLFFAFYNKNDQVRVTDLHQGI
                     VWGTQTSETRLDERLINRFDYDGDYGTVLNRFLMQAAIDYPLTVHGSGQQTRAFIHLQ
                     DTVRCVELAISNPPAAGERVKILNQLTETHRLIDLARLVSEMTGVPIEHVPNPRREAD
                     ANDLAASNEGLVGLGLQPTTLKGGLLEEVTDIARRYAERCDRTRIPCMSHWTTDIAGD
                     IEVIVPSGEASTAG"
     gene            complement(14767..15186)
                     /locus_tag="AC16PP_02635"
     CDS             complement(14767..15186)
                     /locus_tag="AC16PP_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR6480549.1"
                     /translation="MTYDPDNVFAKIIRGQAPAHRVYEDAWTLAFMDLMPQTDGHTLV
                     IPKSEAVSLLDVDSEALARTIQTTQKIAAAVKAAFGAHGVMVAQLSGAAAGQTVFHLH
                     FHVLPRLHGADFEIHAREVAPAEVLAEHAERIRRALG"
     gene            15294..16163
                     /locus_tag="AC16PP_02640"
     CDS             15294..16163
                     /locus_tag="AC16PP_02640"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6480550.1"
                     /translation="MQLKGQVAVVTGGASGMGRDTVLRFLAEGARVVIADFNARTGQA
                     VLEEARAAGFGESTRFVRTDVAREADVEAMCGLAVEAFGRLDVVFNNAGVGGAIGPLT
                     ETTVESWDYTMDVLAKGVFLGIKHAARIMREQGEGGAIINTASIAGLSGDAGPMIYSA
                     AKAAVISLTKSAAVELAPDRIRVNAICPGFIATPLADGGRRDETRERFATSQPWPEHG
                     DGTHIAGAAVFLAGADSRFVTGEALVVDGGLTAAGPELSKRFPRASGRHARVSGVTKG
                     STGEAPELKRLEE"
BASE COUNT         2853 a         5420 c         5118 g         2776 t
ORIGIN      
        1 gcgcatcgga cgccgcgtag ttgctgcgca cgaagtcgct gatgcaggcg cgataccgct
       61 cgacgccttc caggaagcgg tcccaggtca tctcgtcgac gtcgtccggc ggccgctcgg
      121 gaaatgcaca tccatgcacg ggaacgggcg tatgggcggc agccaccggg gccagggcga
      181 cgaggcagat gaaaagcagg cgcatggctg actcctttga gcattgacct ggcacagccg
      241 atgatggcag ctcaacgccc ggtcgattgt gccgccgtgc gcaaaagcgt ggcgacttgc
      301 cagatctccc aaaaagcgta ccgttcccct gagccaattg acagcaggac gaggggaacc
      361 agatgaccga acgctaccag ggataccagc gactggagtt cgactacccc cacccacgga
      421 tactgcgcat cagcttcaac cgcccggagc gctacaacgc cctggacgtc gtcggtcaca
      481 acgagatcac ctacgtctgg cgcgaagtgg acgccgaccc cgacatcgac tgcgtgatcc
      541 tcaccggcaa gggcaaggct ttctcggcag gcggcgattt cgagatgatc gaagagatca
      601 ttgccgactt cgacacccgc gcgcagacct ggaaagaagc gcgcgatctg gtctacaaca
      661 tcatcaactg cggcaagccg atcatatcgg cgatcaacgg gcccgcggtg ggcgcggggc
      721 tcgtcgccgg gcttctcgca gacatctcca tcgtggcgga gtccgtcagc ctgacagacg
      781 gtcacacgcg ccttggcgtc gcggccggag accacgccgc catcatctgg ccgcttcttt
      841 gcgggatggc caaggcaaag tactaccttc tgacctgcga ccggttgtcc ggcgccgagg
      901 ccgagcgcat cggcctggtc tccatgtgcg tgccggacga agagctcgag agccgggccc
      961 tgtcggtcgc cgtgcagctc gccaacggcg ctcccaacgc cattcgctgg accaagtacg
     1021 cgctgaacaa ctggctgcgt ctggccggcc cgaccttcga tgcgtccacg gcactcgaga
     1081 tgctcgggtt cacgagcggc gaggcgaggg agggggtcag ctcactcaag gagaagcggg
     1141 atccggagtt caagaaaggt tcccggatct gaggtcggtg gcgtcagtgg tcgccggtca
     1201 gcggaacgaa cattaccggc agcacgtccc tggtcgccac cgctccgtcg gcgtccttgc
     1261 tgacgagcac caggttctga ccgccatgca cctcgcccac gggaaggatc atccgaccgc
     1321 cgggccgcag ctgctccagc agcggcgcag ggatctctgg cgcaacggcc gttacgacga
     1381 taccgtcgaa cggacctttc tcgggccaac cgtaaaaccc gtcaccgacg cgcacgaaga
     1441 cgttgtcgta gccgagccgg tgcagccgct cggtggccgt ttgcgcgagc gcgggaatga
     1501 tctccaccgt gtaaacctcg cccacgagcc ccgcgagcac ggccgcctga tagcccgacc
     1561 ccgtgccgat ctccagcacc cggtgcgtcg ggtggggctc gacgaagtcg gtcatgagcg
     1621 cgacgatgaa cggctgagaa atggtctgcc cgtgaccaat gggtaagggg acgttctccc
     1681 aggccgcgcc ggggctccag gcctcgacaa actcgtggcg gggcacggcc tcgagcgccg
     1741 ccatcacacg ttcgtcgagg gcacttcggc cggtatagtc cgccgtgcgc ttcgcatcac
     1801 cacggatctc tgccatcagc cggcgcttga gcagctcttc cggctctgcg gcgtgcagcg
     1861 cggacgtcgc ccccatcagg aggaaactcc cgaacgccca ggcaagcagt cctttcgatg
     1921 cagcgcagta catctattta gcatgacatg gctggccgag ccggcccatc cgcattgtcc
     1981 cgtatggatt tgcgcgcccg tcggcaacga catagcctgt cggcatgagc aacgaaaccc
     2041 gctgctgcat cgtcggtgcc ggccccgcag gaatgttcct ggcacttctt ttggcccggc
     2101 gaggagtgac ggtcaccgtc ctcgagctcc atcgcgacct ggaccgcgac ttccgcggag
     2161 ataccgtgca tgcctccacg ctggaggtgc tcgatcagat cggcctggcc gaccgcctgc
     2221 tcgcgctgcc ccacgcaaag atgcgccagg tcactctcca caccgccgcg cggacgctcg
     2281 agctggtgga cttttcgcgg ctgaagacgc gctttcccta cgtgatggtc atgcctcaag
     2341 tcgtcttcct caactacctt cacgagcagg cgaaaagcta cccgggattt cggtgcgttc
     2401 tcggcgccca ggttcaacag ctcgttgagg tggacggacg cgtctcgggc gtccgctatc
     2461 ggcgcgacgg cgaggagcac gagctgcgcg ccgatttggt catcgccgcc gacgggcgct
     2521 tctcgcggct gcgcaagctg gccgggctgg aggccgaaag cctgagcccg cccatggacg
     2581 tctgctggtt ccggctgccc cgggaagcat ccgacggcca cgaggcgggc ggtttcttcg
     2641 tcgggcaagg ccgcatgctc atcgcgctgc cccgttcgga cgagtggcag atcggctacg
     2701 tgtttcccaa gggagacttc aagacgctca gggaatccgg aatccaggcg ttccgggact
     2761 ccatcgcggc aaccgcgccc tggctggccg gacgcaccga cgtcattcac ggctgggaaa
     2821 gggtgcacct gttgacggtg agctctgacc ggctgccgac gtggcaccgg cccgggctcc
     2881 tctgcatcgg ggacgccgcg cacgtcatgt ccccggtggg gggtgtgggc atcaatgcgg
     2941 ccattggcga cgccgtcgag gcagcgaacg ttctgactcg acccctgctg gacgccgtcg
     3001 ctcccggcga agcggccctg gccgaggttc agcggcgccg ggaggttccc acccggatca
     3061 ttcagggctt gcaggcgcgc atacaggaca ccgtggtgtc gcgcgcactg agagacgagg
     3121 agttccatct gccgcttccc gcccggctga tcctcgccac gccggggctg agaaaccttc
     3181 ccccaaggat tttcgcgcag ggcatcaagc cgctaagatt ggaagccctc ccccgcacgg
     3241 agcatcaaag aacatgacgg ggctgcgttt gggcgtggtc ggcgacctgc acacccactg
     3301 ggacgatatc gacgtggcgc agttcaacgc gacgcgctac gacctgctgt tcttcacggg
     3361 cgacctcggt ggcggcagcg tcgagagcag cctgcgggtg gcgcgaacga tctcgcgact
     3421 cgacgtggaa accctggtca tgcccggaaa caacgatacc ggcgacattc accagctggc
     3481 tgccgagctt tcccaccgct caggcctgaa aaggctcacc gggatccgcc agggcgccgg
     3541 cgacgcgatc aagcctattc gcctttgcgg gtacagcagc catcgctatc gcacccccgc
     3601 tgcggacgtg acgctgatcg cggcccggcc ccacagcatg ggcggcccga ccctgacctt
     3661 cgccgaccac atgtctgcca cgtacggtat cagcagcctt gaagcgtctg ccaagcgcct
     3721 gaaggaactg gtcgacgcgg ccgaaaccgc cgacctcgtg ttcctctccc acaacggacc
     3781 cacgggcctg ggcggggagc cggcagacat gtggggctgc gacttcaaac ccgatggggg
     3841 agattggggc gatccggatc tccgagaagc cattgctcac gccgtcgcga ccgggcgacg
     3901 agtgctggcg gtggtcgcgg gccatatgca tctcaagacc aagtgcggcc aggagcgccc
     3961 ctggcgccgc gagcacgacg ggatcctcta cgtcaacgcc gcccgggttc cgcggatcta
     4021 ctccggcagc gacgacgtca tgcgccacca cgtctcgctg cacattcacg aacagggcat
     4081 cgagatcgac gagttggagg tcccccaggg gctgggctaa accagggtcc cgagtatggc
     4141 ttccatgcgt cggtccgaga tcccgtagta ggtccgcatc agatcgagca cctcgttctc
     4201 ttccaccgcg acacgatcat ctgccagcgc gatgacggcg gcgagcgtaa acgccatttc
     4261 ccgatcgtcc cggtccgcgc tcaaccgagc tccgatccgc gccacgcaat cttctcttcc
     4321 ttcccgacga acgcgctcag cgaattcggc cagcagcgcg tccacggctt cgcggcccat
     4381 cagaccatcg gtgagcatgt cgaccgcaac caccagggcg tcgtgttccg aaacggcctg
     4441 ttcggcatcc gccagcatca ccagaaacat ggtctcggca aaaggctcta cccgcaggcg
     4501 gaccgcatcg ccgggctgct catcgggaac ttggccggca gccccgagat cgctggagag
     4561 caggtgatcg cgcagccgca caatggcgtc ggttcgaatg ttcattcggg cacctcgatt
     4621 ccggctcttg ccagcgccgt tttgaaacca gagtacctgg gctgatcgat ggcgatctgg
     4681 ttcaccacgg tggtgcggag gtgatccgtg agggcaacgt cgtcgagcgc catctcgccg
     4741 gatcgcagcc ggtggacgag ctcccagcgc agggcgtcca gatcccccga acgccccagc
     4801 agcgcttcca gccggacctg ctcacggaac ctcgcctcgg ctcccgtcgc cagctctcgc
     4861 agcacgatgt ccagggagtt gccagccacc cgtgccagaa agttcgtccg cccgctcgta
     4921 gccgccatga cgtcggcgcg caggtaatcg cgcacgctca ccagcagctc gtcgacccgg
     4981 ggcatgtccg tgcaatcctc aagatccgtg cccgccacca gctccacgtc accaggaatg
     5041 atcaggttga cgcaatccac ctggcactcc gacgagcgcc ggccgatggc cggccgctcc
     5101 acggtcttgt cggggccggt acggtagtgc tcggccatgc ccagacagcc gaccgcccac
     5161 cagaacgacc ccatcacctc ccagaacttc acgcgctcgg ggtcaactgc cacgccggac
     5221 tcgtcctcgt agccgcggaa cagatcggcg tactcgccga atccacccac cggccggtcg
     5281 ctgcgtccga aacgccagga gttggtgcag atccagccga ggtcgcgcat gggatcgccg
     5341 atgtgggcga cctcccagtc gagcacggcg acgatcccgt tcgcgtccac catgaaattg
     5401 ccgttgcgaa aatcgttgtg gacgagcgcc atgtcgaacg tcgccggcat gttgtcgagc
     5461 agccagcgcg ccgtgaagtc gatcatcggc tgaggcgtgg gaaagtcctt gtagcgatcc
     5521 cagctgcgat gcagcagctc ctccggagtc agagtttcca gccgctccct gagcccggca
     5581 cggtccaggt cgatggcgtg aatcttcgcc atcactcgcc cgcactcgta agcgagctgc
     5641 ggccgaacgc cgtcgagctc gcgcgcccgg acgatgcgcg cgccaagcgc ttcgccctcc
     5701 agccactcca tcacgaaacc ttcgcccagg ccgtcctccg gctccagaac gtaatagacc
     5761 tcgggctccg ggaccccggc ggcgcgggca ctgcgcatca gcagggcttc ggtggccagc
     5821 gtcggtggcg caacctccac gtccaagccg cccccgggag cccggcgcat ggccatgagc
     5881 tcctcgccgt cagccgtatc gacggtaagc cgatacgtct cctggctggc tcccccggac
     5941 aggcgctcgg cagagcgcag cgagcggacg cgcccgatcc gaagagccag caccttgcca
     6001 agccgcgctt cgaactcggc gtggtccacc ttcagctttc cctgacaccc ttgggcgcgc
     6061 gctgcgacat gaatccgaac atgtatccgg ctacccgacg catctggatt tcctccgcgc
     6121 cctcggtgat ccggtagcgc cgatggtgcc gatagatgtg ctcgaaaggc ttgtgccgcg
     6181 agtaccccag accgccgtga acctgcatgg cgcgatcggc cgattcgcag cacagacggt
     6241 tcgaccagta gttacacatg gacaccttgt ccgaaacgga gaacgcaccg tacgtatcca
     6301 tttcccaggc ggttttgtgg atgagggccc ggagcatctc gcactgggtc tgcagctcga
     6361 cgaggggaaa ctggataccc tggttggtcg caagcgcctt gccgaacggc tttcgttcct
     6421 gcgcatacct gacagactcg ttgatgcaga actgggcggc cccgaggctg gaggcggcct
     6481 gacggatgcg gttctcgttg aagaagtgct gaaccacctg cagcccgcgc ccttcgcccc
     6541 cgaaaatcga aacgtggggc acgcgcacgt tggtgaacga tacccggccg tgatccgtgg
     6601 gcatgttgaa cgtccacagc atctcctcga tcttgacgcc atcggcttcc atgggcgtca
     6661 ggaacgcggt gatgccgtcc ccgtccccgg gcttgcccga cgtccgcgcc atcaccatgt
     6721 cgtacttggc cttgtgaatg cccgtgttcc aggtcttctc cccgttgatc acccactcgt
     6781 cgccgtcacg ctcggcgtgg gtctccatat gggtggcatc gctgccgtgg ttcggctcgg
     6841 tgatgccgaa ggcgaagccg cggacgccgt tcgcgagatc gtcgacccac tcggccttct
     6901 gctcatcggt gccgtattcg agcatgagca gcaggccgac ctggttgccg acgatggaat
     6961 gctcgttctg cagatcgttg tgcagaccca ggcctttgac ggcgagatgc tcccggatga
     7021 tggccatgcc caggttgttc ccgttcatac caccgtactc cttcggaaag gggtagcggt
     7081 agaagccggc ctcgtctgca agacgcttca ctttgaaaag cagctgctcc cattcctcgt
     7141 ttggcagccc gccccgatcc cagtccgttc gggcgtcttc gcggcggtgg tcgaagaagc
     7201 ggatgttgtc gtcctgctgc tccagcggct tgatcacctg ctcgatgaag tcgtccaggc
     7261 ggtccagcag ctcggcgatg tcttccggga tctcgaagtt catatgggtc cccttaaatg
     7321 ttcgtttggc attccccgca gcccaattaa aatgacctga ccatcggatt gcaaagggcc
     7381 tgcgccttga acgtaagcat cacctactgc gtacagtgaa actaccaacc caaggcggtc
     7441 agtctggctg ccaggatacg agaagccttc ggtgtcgagg ccgagctcgt cggcgggggc
     7501 agcgggatat tcgatgtggt cgtggacggc cgcaccgttt actccaagca cgaaaccggc
     7561 cagtttccgg acgaggcgga cctggtcgct tccctcggcg gctgaccggc gaggctggcg
     7621 gcggcgccct cagtccttga ggaggttcag aaactccgtg cgcgtgctgg gattctcgcg
     7681 gaaggccccg agcatggcgg aagttttcat cagagagttc tgcttctcca ccccgcgcat
     7741 catcatgcag aggtgcctgg cctcgatgat cacggcgacg tcggcagctc ccgtgacctc
     7801 cgtcagcgcg ttcgcgatct gaaacgtcag ctcttcctga atctgcagcc tgcgcgcaaa
     7861 catgtcgacg atgcgggcaa ccttcgagag gccaagtacc tttccgtccg gaatgtagcc
     7921 gacgtggcag cgaccgatga agggcagcag gtgatgctcg cagaggctgt agagctcgat
     7981 atcctgaacg acgaccattt cccggttgtc ggaggggaac agcgcgtcgt tcacgacgtc
     8041 gtcgagcgtc tgctggtagc cacgggtgag aaacagcatc gagtccgccg cgcggcgcgg
     8101 cgtatcgacc acgccggggc gatcgatgtc gtccacttct tcgatgatgg tcgtgaaagc
     8161 ctctaggagc ctgtcgcggt cacgcatgtc gtttgctccc ccggattaat gattgtcgag
     8221 aggtgatagg gcttcagcgc ccaacgccgc cagagcggca tcgaggggca ggacttcctg
     8281 ggccttgcca ccgagccgac ggatcgccac ggtgccttct tcagcctcgc gcttgcccac
     8341 caccagcatc acaggaacct tggtgacgct gtgctcccgg accttgtaag agatcttctc
     8401 gttgcgcaga tcggcttcca cccgaagccc cgccgcccgg gcggccgcca tgacggaccg
     8461 agcgtattcg tcggcctcgt ccgtgatcgt cgcgaccacc gcctgcagcg gcgcgagcca
     8521 caacggcagg tggcccgcgt agtgctcgat caggatgccg cagaagcgct ccagggagcc
     8581 gaacatggct cgatggatca tgaccgggta ttgacggctg ccatcggcgg ccacgtacgt
     8641 cgcgccgagc cgctcgggca ggttgaggtc tacctgaacg gtgccgcact gccagtcgcg
     8701 cccgatggca tcgcgcagaa cgaactccag cttcggcccg tagaaggctc cctcgccggg
     8761 attcagcgtc cactggagcc ccgaggcttc cagggccgtt atcagcgcgg attcggcgtt
     8821 gtcccagacc tcgtccgagc cgacgcgccg ctcggggcgg tccgcgtact tcaccgtcac
     8881 gtcctcgaat ccgaaatcac gatagatttc gatgatgagg ttggatgcct tcacgcattc
     8941 ctcggtgatc tgctccgccg tgcagaagat gtgcgcgtcg tcctggacga acgcccggac
     9001 gcgcatcagc ccgtgcaggg cccctgaggg ctcatagcga tgcaccttgc cgaactcggc
     9061 cagatacagg ggcagatccc gataactctt gatgccctgc ctgaagatct gcacgtgacc
     9121 cgggcagttc atcggcttga tggcgtagac gcgatcgtcg ggcgtctgcg tgaggtacat
     9181 gttctcgcca aacttctcca gatggccgga acgttcccag agcgagcggt cgagaatctc
     9241 gggggtgttc acctcctgat atcccgcctc gatctgcttg cggcgcatgt agtcgatcat
     9301 gcgctggaac atggtccagc ccttggggtg ccagaacacg gctccgggac cctcttcctg
     9361 gaggtggaac agatccattt cccggccgag ccgccggtga tcgcgcttct cggcttcctc
     9421 cagccgcacc agatatgcgt taagatcctt gtcagtcgcc catgccgtcc cgtaaatacg
     9481 ctgaagcatc tcgttgcggg agtcgccccg ccagtaagcg ccggccaccg acatgagctt
     9541 gaaagcaccg ccaagccggc ccgtggactc caggtgcggg ccccggcaga gatccaggaa
     9601 ttcgccctgg cggtacagac cgacctcctc gccctcggga atgccggcaa tgatctccgc
     9661 tttgtaggac tcgccgatgg actggaagta agccacggct tcgtcccggt cccagacctc
     9721 gcggcggatc tcctcgttgc gcgcgacgat ctcccgcatg cgggcctcga tggtctccag
     9781 atcctctggc gtgaacggct ccgcccggga aaagtcgtag taaaagccgt tttcgatggt
     9841 gggcccgatg gtcacctggg tctccgggta gagttccttg accgcctccg ccagcacgtg
     9901 ggccgcgtcg tgacgcagaa ggtccacacc ggcctgggag tctcgagtga tgacctccac
     9961 cgccgcgtcc tgctcgatgg ggatgtagat gtccttgagc tcaccgtcca cgcgaacggc
    10021 caccgccgcc ttggccagcg acttggagat atccctggcc agctccgtgc cggtcaccga
    10081 cccgtcgaac tggcgctggg aaccgtcggg cagggtgatg gataccatta gaaaagacct
    10141 ggtggccgaa aaacggcgat tttgccaaac ggccccgccg cggtcgacgt gtaattgatc
    10201 agccgcctgc ccgaatgagg tctgcggcct tctccgcgat catgatggtc gccgcattgg
    10261 tattgcccga aaccacgaaa ggcatgacgg acgcatccac gacccgaagc ccggagaccc
    10321 ccagaacctt caggttctca tccaccacgg atccgatgcg gcacgtgctc gtgggatggt
    10381 aggtggtggc agcgtcgccg cggatgtact cgagcagcgc ctcgtcagag gtgacgccgg
    10441 gttcgggcag gagacgctca gttacgtacg gccgcatcgc cgtgctctcg aagatgtcga
    10501 taatcattcg cagacccctg acgagggtat cccggtcgag ctcgctgtcg agatagttgg
    10561 gccggatggc cggcggttgc ctcgggtcgg cgctggtgat gtggatcgag cccacggact
    10621 caggccgact ctggttgacc accgcggtca tggcgggcac cggcatggcc cggatacgcc
    10681 cttcctcgtt gtaaagcgtt gcgccggaag cgaaatgaac ctgcacgtcg ggggcgtcga
    10741 ggccgtcacg cgtgtaccag aagccgccga caggagccga tccggtggtc aacggtccac
    10801 gcctgagcat gagccatttc aaggcggcaa cgccgacccg ccacccctgc acctcccggt
    10861 tgatcgattg ctcgggatga gtcccgtaaa ccgtcttggt cagaaggtga tcctggaggt
    10921 tctcgccgac ctcgggcagg tcaccgacga cctcgatgcc gagcccccgc agccgctcgc
    10981 cgtccccgac tccggacagc tcgaggagct gcggcgagtt gatcgcgccg ctggccagga
    11041 tgacttccct ggcagctacc cgcgtggcgg gggatcgggc ccgctcgatc tccaggcccg
    11101 tggccgcctt cccgtcgaag catacccgcc tgacgaggac gcccgtcatc acgtcgaggt
    11161 tgggccggcg tctggcggga ccgaggtagg cggttgccgt gctccagcgc cgaccccgga
    11221 gctgattgaa ctggtagtaa ccgcaaccgg cctgctcctg cccgttgaaa tcgtcgttgc
    11281 gcggaatgcc cagctccgtc atgccgtcga tgaacgcatc catggccgct cgcctgtcgc
    11341 gcacgtcctg gacgtgcagg ggtccctccg cgccgtgaaa ctcgtcggcc acccgctgct
    11401 ggcgctcggc cttacggaaa tacggaagca cctcctcgta gctccagccc cgagcccccg
    11461 actgggccca gcggtcgaag tccaggggaa cgccgcgaat gtagatcatc ccgttgatgg
    11521 cgctggagcc acccagaacc ttgccccgag gccagtagat ggagcgcccg ttcagggccg
    11581 gctcgggctc agtggaaaaa ccccagtcca ccttcgggtt gcggaagttg gtcgcccacc
    11641 ccgcaggaat gtgcagcagc gggttcccgt tgcccggccc cgcctcgatc aggagcacgc
    11701 gcgtctccgg gtccgccgac aaccggttgg ccagaacgca gccggccgag ccggctccca
    11761 cgatggcgta gtcgtaggcg tccatgaccg gtttcgcgct tcaggggctc gtgacggggc
    11821 ggatcaacgg tccagggccc ggttccattc ctctatgcga tccgcgtggg cttctagcag
    11881 agcgtccagg tccccctcga tcaccacgct ttcaatgacg tcgccctggg cgatgctgtc
    11941 caccacggcc tgatcttccg gcccctgaac ctcgccgaag atggtgtggt tgccgtcgag
    12001 ccagggagtg ggtacgtggg tgatgaagaa ctgcgagccg ttggtgccgg ggccggcgtt
    12061 ggccatggat agccgaccgg ggcgatcgtg gcggacctcc gagtcgaact cgtcctcgaa
    12121 gcggtagccg ggaccgccgg tccccgtacc cagcggacag cctccctgaa ccatgaagtc
    12181 ggcgatcacg cgatggaatt tcagaccatc gtaaaaaccc cgccgggcaa ggttcgcgaa
    12241 gttcgctacg gttaccggag cccgctcggg catcagggaa acctgaatgg ctcctttgga
    12301 agtgttgaag gtggcttgca gagtcacgtt agctcctgtg gggtaacttg aagaggctcg
    12361 agagccgtat cggcggccag tgaaacacaa cgggcaggcg ttttcgagcg gcgccaacgg
    12421 cccaggcggc caaaccccca gcgggttgta gccgccctcc cagtcccgct actatcctcg
    12481 tcaagcgacc gcaccgcggc gattagagag ataacgtgcg gctactgtta gtcacagatg
    12541 cctggttccc acaggtgaac ggcgtggttc gaacgctgtc caccaccatg gaaaatctcg
    12601 cgcggctggg ccacgagacc gaggtgatcg cccctgcgcg cttcaaaagc gtcccctgtc
    12661 cgacctaccc ggagatccgg ctcgcgctgt gcactccggg ccgggtcggg aacatgatcg
    12721 ccgagttcgc gccggacgcg gtgcacgtcg ccacggaagg gccactgggg ctggccgccc
    12781 gctggtggct caagcgccgc ggcataccgt tcaccacctc gttccacacc atgttcccgc
    12841 agtacgtcag gctccggttc gggctgcctg aagcatggac gtttgcggcc atgcgccgct
    12901 tccacggggc cgcgactcag accatgtact ccacgccccg gctgcgcgac atgctccgcg
    12961 gccagggctt cgccaacctc gtcggctggg tgcgcggcgt ggacacggag ctgttcaagc
    13021 cgggtccggc ggaacccctc gatctgccgc ggcctttgca gatgtacgtc ggccgggttg
    13081 ccgtggagaa gagcctcgag gactttctgt ccctggacac ccccggcacc aaggtgatca
    13141 tcggcgacgg gccccagcgg ggagagcttg ccgcacgata tccccaagca cgtttcctgg
    13201 gcccccgcta cggcgacgac ctggtccgct gctaccgggc agccgacgtg ttcgtgttcc
    13261 catcgcgcac cgacaccctg ggactggtga tgctggaagc gatggcctgc ggcgtgccgg
    13321 tcgctgcgtt tccggtgcag ggcccattgg acgtggtcgc cgacagcggc gcaggagtgc
    13381 tgcacgacga cctcggcacc gccatcgctc aggccatcgg catcgacccc gaaatctgcc
    13441 gagcccgggc catggagcac tcgtgggatc agtccgtgcg cgaattccac gcaaacctgg
    13501 tacccatcgg acagaccgcc taccatttcc gcggcgaagg ggagaccgca tgaaaattct
    13561 cattctcggc ggggacggct tctgcggctg gcctaccgcc ctgcatctgt cggcacaggg
    13621 tcattgcgtg gtcatcgtcg acaacctctc caggcgtaac atcgacaacg agctcgaagt
    13681 tcagagcctt accccaatcc gcccgctggg cgagcgcatc ggcacctggt acgagctgac
    13741 gggacgggaa atccagttcg tgcgtatgga tgtggcgacg gagtacgacc ggttgctgga
    13801 gctgatccgc agccatcgcc cggacgccat cgtgcacttt gccgagcagc gtgccgcacc
    13861 gtactccatg aagtcttcct ggcacaaacg ctacacggtc aacaacaacc tcaacgccac
    13921 caacaacgtt ctggccgcca tcgtcgacag ccgccaggac atccacctgg tgcacctggg
    13981 caccatgggc gtctacggct actccagcgt gggcgtgaag ctgccggaag gctatctgaa
    14041 cgtgaagatg accaacgacg acggcgagga ggtggagcag gagatcctct atccggcgaa
    14101 cccgggcagc atctaccaca tgaccaaaac ccaggatcag ctgttctttg ccttctacaa
    14161 caagaacgac caggtgcgcg tcaccgacct gcaccagggc attgtctggg gtacccagac
    14221 gagcgagacc cgcctggacg agcggctgat caaccgcttt gactacgacg gcgattacgg
    14281 cacggtcctc aaccgtttcc tgatgcaggc cgccatcgac taccccctca ccgttcacgg
    14341 cagcgggcag cagacgcgag ccttcatcca ccttcaggac acggtgcgct gcgtcgagct
    14401 cgccatatcc aacccgccgg ccgcgggcga gcgggtgaag attctgaacc agctgacgga
    14461 aacccaccgg ctgatcgatc tcgcccggct ggtctcggag atgacgggcg tgcccatcga
    14521 gcacgtgccg aatccgcgtc gggaggccga cgcaaacgat ctggccgcga gcaacgaagg
    14581 gctggtgggg ctcggcctgc agccgacgac gctgaaaggc ggcctgctcg aagaggtgac
    14641 cgacatcgcc cggcgctatg ccgaacgctg cgaccggacg cgcatcccct gcatgtccca
    14701 ctggacgacg gacattgcgg gggacatcga ggtgatcgtc cccagcggcg aggcttcgac
    14761 cgccggctag ccgagcgcgc ggcgaatacg ctctgcgtgc tcggcgagca cctcggcagg
    14821 cgccacctcc ctggcgtgaa tttcgaagtc ggcgccatgc agcctgggca gcacgtgaaa
    14881 gtgaaggtga aagaccgtct gcccggcggc cgccccgctc agctgagcca ccatcacccc
    14941 atgggcgcca aatgccgcct tcaccgccgc ggcgatcttc tgggtcgtct ggatcgtccg
    15001 ggcaagcgcc tcggagtcca catccagcag gctcaccgcc tcgctcttgg gaatgacgag
    15061 ggtgtggccg tccgtctgcg gcatgaggtc catgaacgcc agcgtccagg cgtcctcgta
    15121 aacccggtgg gcgggcgcct ggccgcggat gatcttggcg aagacattgt cgggatcgta
    15181 agtcatcgtt gctccggtgt tactccggtc tccggttcag acgtcgagca tattcgaaaa
    15241 ggcgcgtcat cgggcaccgg tggtaatctg tcgttttgcg cggggggaat gcgatgcagc
    15301 taaaaggcca ggtcgccgtg gtgacgggcg gcgccagcgg catgggccgc gacacggtac
    15361 tgcgttttct tgccgagggc gcacgcgtgg tcatcgcgga cttcaacgcc cggacgggcc
    15421 aggccgttct cgaagaggcg cgcgccgccg gattcggcga gtcgacgcga ttcgttcgca
    15481 ccgacgtggc gcgggaggcg gacgtggagg ccatgtgcgg tctggctgtg gaagccttcg
    15541 gccggctcga cgtcgtgttc aacaacgccg gggtcggcgg cgcgatcggc ccgttgaccg
    15601 agaccacggt agagtcatgg gattacacca tggacgtgct tgccaaaggc gtcttcctgg
    15661 ggatcaagca cgccgcacgg atcatgcgtg agcagggcga aggcggtgcc atcatcaaca
    15721 ccgcgtccat tgcagggttg agcggcgatg ccggcccaat gatctactct gcggccaagg
    15781 ccgccgtcat cagcctcacc aagtccgccg cggtggagct ggcaccggac cggattcgcg
    15841 tgaacgccat ctgcccgggc ttcatcgcca cgccgctggc cgacggcggc cggcgggatg
    15901 aaaccaggga gcgctttgcc acgtcacagc cctggcccga gcacggcgat ggcacccaca
    15961 ttgccggggc ggccgtgttc ctggccgggg ctgattcgcg attcgtcacc ggtgaagcgc
    16021 tcgtcgtcga tggcgggctg acagccgcgg gacccgagct ctccaagcgc tttccaaggg
    16081 cctccggccg ccacgcccgg gtgagcggcg tcaccaaggg cagcacggga gaagcaccgg
    16141 agctcaagcg tctggaggag tagagct
//
LOCUS       DBNHKV010000084        10595 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1287957, whole genome shotgun sequence.
ACCESSION   DBNHKV010000084 DBNHKV010000000
VERSION     DBNHKV010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10595
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1287957"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="AC16PP_02645"
     CDS             complement(<1..856)
                     /locus_tag="AC16PP_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase-like protein"
                     /protein_id="HIR6480551.1"
                     /translation="MIDWITPWELSRRGILGMNRRNIEFIGRYNRREQYPLVDDKLKT
                     KLIAQHAGVQVPDLIDVVRTQHEIETIDDKLADLSEFVIKPAQGSGGKGILVIERHEA
                     GVFTKSSGEQIGILDVKRHLSNVISGLHSLGGRADVAILESLVRVSPLFRNVSYEGVP
                     DIRLIVFQGYPVMGMLRLATLASDGKANLHQGAVGVGLDIGTGKSVAAVQHNRIVRNH
                     PDTGAELTELSVPNWRQLLELAARCFEVTQLGYLGCDIVIDELRGPLLLELNARPGLS
                     IQLANDAGL"
     gene            complement(853..2343)
                     /locus_tag="AC16PP_02650"
     CDS             complement(853..2343)
                     /locus_tag="AC16PP_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inactive transglutaminase family protein"
                     /protein_id="HIR6480552.1"
                     /translation="MSQKGPFLFLVALLCIAGVGTAAFRHAEYRIPWLPGEQQNVWEI
                     EARIEYMAQGVPTQAFLTLPPNQDGFRVVNETGASSGFGFEVETQRRQRRAHWSKREA
                     NGPQTLFFKLELVRDAEYRVDGPPPAAPPASALDEPYQTAADQIVQAALPVSANARTL
                     AQQLINRMLLVPADQTVSLLRDRFDEATLLANLLVTAGANARTVQVLELEDGRRRQSL
                     KDYVQVWDDGRWYLFNPTVGPVTDLDNLLLWQTDTPSVLEVVGGTRSRVSFSMISQPR
                     SMLALAEPSTGTGFSLHSLPIAEQGMFKLIMLLPVGALMVVLMRIIVGIRTAGTFMPV
                     LIALAFLQTELLPGIVSFVLVIAIGLVIRSYLSHLNLLLVARIATLVILVIGMISVFS
                     IDSYRLGLIGGLTITFFPMIILAWTIERMSIIWEEEGPREVFIQGGGSLLVAVLAFLF
                     MDLTAVRHMAFNFPELHLAVLGLVMLVGRYTGYRLLELRRFIGFKQ"
     gene            complement(2340..3167)
                     /locus_tag="AC16PP_02655"
     CDS             complement(2340..3167)
                     /locus_tag="AC16PP_02655"
                     /inference="COORDINATES: protein motif:HMM:NF017434.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease family protein"
                     /protein_id="HIR6480553.1"
                     /translation="MNRPRTAFEDVRRGAMAYRAGPGRALLRAPLLALLMSGCAVTTG
                     SQDNPNAALVSRVQALEYQVLATQVAVRELAEDQQSGRADLSRQMGDLNTELGRVPEA
                     VGAICASAETAAAAACDSDQPIQTVVMSGDKMVVGEMERVWLEPPGTTLIARVDTGAH
                     SSSLHAENLVEFERDGEEWVRFDLILDDEIKTLERRVVRYVRVYQQADPEGTRRPVVT
                     MRLRIGDVHDSFEFTLADRSHLDFQLLLGRNFLADMALVDVGKQFVQPPYQPPSTSR"
     gene            3272..4390
                     /locus_tag="AC16PP_02660"
     CDS             3272..4390
                     /locus_tag="AC16PP_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HIR6480554.1"
                     /translation="MAERNRAQNPPPPSRSDNGWLKGRGATKNEASRYLARHSVPVDD
                     GWSANEVDEPDGSRVATELYPDRTVTLITRNASPDIPFDRSINPYKGCEHGCVYCFAR
                     PTHAYLDLSPGLDFETKIFFKTNVRERLVDELGKRRYQCRTIAMGTNTDPYQPAERHR
                     RITRSVLETLLEARHPVSIVTKSQLILRDLDLLQELARQQLATVAVSLTTLSNDLKTR
                     LEPRTAGPGARLRVIRELRGAGVPVGVMVAPVIPFVNDHEIEDIVAAATDAGARTANY
                     ILLRLPLEVSGLFEAWLGVHFPDRAERVMNAIRSARGGKTYDATWGKRMRGEGEVAEL
                     IAARFKVALRRNGILGATQPPLRTDLFRPPGGSQLSLI"
     gene            4423..5991
                     /locus_tag="AC16PP_02665"
     CDS             4423..5991
                     /locus_tag="AC16PP_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR6480555.1"
                     /translation="MDLIGWSWLFLVLYLALMLAFGFIGRRRIKSADDFATARESYGP
                     VFLAFAFAATTASGATFVGFPGIAYDAGLPAVWSVFLYPAGVYLGVLICLKVVSNTGH
                     EFGSRSIPEYLGTRYQSDWMRVLVSLFSLLLFFYLAGQLVSGIVMFEIMLGVSPDWAL
                     AITAAVLMVYVVLGGAHADILTDGVQGLIMVAIAVGLVFLFVSGFGVDGGFTGMIDSL
                     ERQDANLVGWLNPDYVLYHSWWSVAAVLLAHIPLGMLPHIGNKLWALEQPSQRRRFIR
                     LAFTFGLALGMLGLGGLLARAILGDALLQPGESSNTALSMLFVELFPGWLAALLGIGI
                     LAAVMSTADGLVVSSSQIIANDLYRCTIVPRFARELPERTVDRQVLIISRIATVAVMA
                     ICTAMAWALADRNVALIVWIGTGGMMAAFAGPLVVGAVWRGVTKAGAFAGLVGGVTVF
                     SIAHGGLVDPGWFEPGALRDAAAWLLREAPNPWSCAAMGEIVSIALTWGVSKLTQPLP
                     SDHLTRMFGPVGQE"
     gene            complement(5956..7326)
                     /locus_tag="AC16PP_02670"
     CDS             complement(5956..7326)
                     /locus_tag="AC16PP_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR6480556.1"
                     /translation="MPASPDQPRFARVLKSREVITLALGAMIGWSWVLMTGFWVQTAG
                     SLGTLIAFAAGGLAIALIGLTYSELASALPRAGGEHVYTHRALGHRWSFVCTWALLFA
                     YVNVCLFESIALPTAVEYLLPGIRMGTLWSVLGADVDVGFVIVGAGSALLVTVVNVLG
                     IKAAARLQSAAIGLILLAGVILVSGAVVFGETDNARPWIATPATGILSVLIMVPAMLV
                     GFDVIPQSAEEINLQPARIGKLLIVSVLLAVAWYGLIAFAVATALSKPELATSSMATG
                     DAATALWGDPIAGRLLVLGGVAGILTSWNAFVIGGSRVMFALAESGSIPAIFARLHPR
                     YRTPYVGVITIGVMCCFAPLFGRTVLVWLIDAGSFAVVVAYLFVPLAFLALRRQEPDL
                     PRPFKVSHPRLVGVGAFVLAIGLLTAYLPGSPSALIWPYEWAIVLAWTIVGAVLFLAY
                     GAKHPR"
     gene            7419..7679
                     /locus_tag="AC16PP_02675"
     CDS             7419..7679
                     /locus_tag="AC16PP_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480557.1"
                     /translation="MPDVVALSHRGKEFEIRLADDGALELHVDGCLRKRREPTGREPQ
                     YVWTNVELQWEEHHYIEARYWAGNRRLKVTVNGDGIYDRSLS"
     gene            7792..9225
                     /locus_tag="AC16PP_02680"
     CDS             7792..9225
                     /locus_tag="AC16PP_02680"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020305637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006081 - aldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HIR6480558.1"
                     /translation="MQDMTLEQMLALLERQREDYLGEGTVSLAKRVDRLERAVGLLKT
                     HEKRLVEAMTADFGHRSEHQSMLTDVAGSIGPLRHAQKHLARWMRPERRKVGPFPLNV
                     LGAKARVEYQPLGVVGVISPWNFPVNLTFTPLAGILGAGNRCMIKPSEYTPATSEVMA
                     EIFPTAFDELEIAVVTGGPQVGADFSGLPFDHLLFTGATSIARHVMRAASENLVPVTL
                     ELGGKSPVVVGRSADIQKTTDAIMTGKMMNAGQICLAPDYVFVPEERVDEFVESSARS
                     VEKMYPTLLDNPDYTSVINQRHYDRLQGYVEEAREKGAKVVEVNPANEDFRQQPHHKI
                     PPTLIVDPDESLQVMQDEIFGPILPVKGYANVADTIDYINHHPRPLGLYYFGDDREEE
                     GKVLSHTTSGGVTVNDVVMHVAQEDLPFGGVGPSGMGAYHGHDGFKTFSHAKSIFTQS
                     KVNVAELAGLRPPYGEKMLKAIRAQMR"
     gene            9233..10006
                     /locus_tag="AC16PP_02685"
     CDS             9233..10006
                     /locus_tag="AC16PP_02685"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6480559.1"
                     /translation="MESGSSEIDRLEPRAFERDGFVVVRGCCDPGLVRRIRDRASSYL
                     QPLLGPAEFEADVGYPGSPASRASDGGDTCRRLLHAYARDSVFRDWATGSSVGAVLRV
                     LFGGADVMMSQCHHNCVMTKHPGYSSATLWHQDIRYWSFDRPELVSVWLALGEETEEN
                     GALKVIPGSHAMNIDRGRLDRDLFLRPELAENAELVEHAQTVPLEAGDVLFFHCRLFH
                     AAGKNLTDEVKLSVVTTYHTADNRPIPDTRSAQYPGVPL"
     gene            10014..>10595
                     /gene="smrA"
                     /locus_tag="AC16PP_02690"
     CDS             10014..>10595
                     /gene="smrA"
                     /locus_tag="AC16PP_02690"
                     /inference="COORDINATES: protein motif:HMM:NF033154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HIR6480560.1"
                     /translation="MGDEDVFREAMSDVAPLQRKQRAQPERGPVEATSAQLERREFAL
                     GNRDREAADPNYLTLGEVPQRQPREIVEWKKDGVQNAVFARLKAGKYPVEGSVDLHRQ
                     TVREARAAVFRFLNLAMAKGWRMVLIAHGRGEQSETPARIKSYVAAWLAQVPEVIAYH
                     SAQNNQGGTGACYVLLKKSPQARENNREQYGGKG"
BASE COUNT         1867 a         3394 c         3540 g         1794 t
ORIGIN      
        1 ccagccccgc gtcgttggcc agctggatgc tcagccccgg ccgagcgttc agctccagca
       61 gcagcggccc gcgaagctcg tcgatgacga tgtcgcagcc taggtagccc agctgtgtca
      121 cctcgaagca tcgggcagcc agctccagaa gctggcgcca gttcggaacg ctcagctcgg
      181 tcagctcggc ccctgtatcg gggtgattgc gaacgatccg gttgtgctgc acggcggcca
      241 cgctcttgcc cgtgccgatg tcgagcccga cccctaccgc accctgatgc agattcgcct
      301 tgccgtcgga ggccagagtg gccaggcgca gcatgcccat gaccggatag ccctgaaaga
      361 cgatcagccg gatgtctgga acaccctcgt agctcacgtt ccggaacagc gggctgacgc
      421 gcaccagaga ttccagaata gccacatcgg cgcgcccgcc cagggagtgc agcccgctga
      481 tgacgttgga gaggtggcgt ttgacgtcca gaatgccgat ctgctcgccg ctgctcttgg
      541 tgaagacccc ggcctcgtgg cgctctatca cgagaatgcc cttgccgccg ctgccctggg
      601 ccggcttgat cacgaactcc gacaggtctg cgagcttgtc gtcgatggtt tcgatctcgt
      661 gctgggtccg gaccacgtcg atgagatcgg gcacctggac gcccgcgtgc tgggcgatga
      721 gcttggtctt gagcttgtcg tcgaccagtg ggtactgctc gcggcggttg tagcgcccga
      781 tgaactcgat gttgcgtcgg ttcatgccca ggatgccgcg tcgcgacagc tcccagggtg
      841 tgatccagtc aatcactgct tgaaaccgat gaacctgcgc agctcgagca ggcggtaacc
      901 cgtgtagcgg ccgaccagca tgacgagccc gagcacggcc aggtgcagtt cggggaaatt
      961 gaaggccatg tgccggacgg cggtgagatc catgaacagg aaggcgagca cggccaccag
     1021 gaggctccca ccgccctgaa tgaacacctc ccgcggcccc tcttcctccc agatgatgga
     1081 catccgttcg atggtccagg ccaggatgat catggggaag aaggtgatgg tcagcccgcc
     1141 gatgagaccg aggcggtaac tgtcgatgct gaacacgctg atcatgccga tcaccagaat
     1201 caccagcgtg gcgatgcgcg ccaccagcag caggttcaaa tgggacaggt aggatcggat
     1261 gaccaggccg atggcgatga ccagaacgaa gctgacgatg cccggcagca gctccgtctg
     1321 caggaaagcc agggcgatca gcaccggcat gaaggtgccc gccgtgcgga tgccgacgat
     1381 gatccgcatg agcacaacca tcagggcgcc caccggcagc agcatgatca gcttgaacat
     1441 gccctgctcg gcgatgggca ggctgtgcag cgagaagccg gtccctgtgg aaggctcggc
     1501 cagggccagc atggagcggg gctggctgat catggagaaa gacacgcggc ttcgcgtccc
     1561 gccaaccacc tccagcaccg agggcgtgtc ggtctgccat agcaggagat tgtccaggtc
     1621 cgtcaccggc cctaccgtgg ggttgaagag gtaccagcgg ccgtcgtccc atacctgaac
     1681 gtagtctttg aggctctgtc ggcgtcgtcc atcctcgagc tccagcacct gcaccgtccg
     1741 cgcgttcgcg ccggcggtga ccagcaggtt ggccagcagg gtcgcctcgt cgaagcggtc
     1801 gcgcagaagg ctgacggtct ggtcggcggg caccagcagc atgcggttga tcagctgttg
     1861 tgccagggtg cgcgcgttgg cgctgactgg cagggcggcc tggacgatct gatcggccgc
     1921 cgtctggtac ggctcgtcca gggcgctggc cggcggcgct gccggcgggg ggccgtccac
     1981 ccggtattcc gcatcgcgca cgagctccag cttgaagaaa agggtttgcg gcccgttcgc
     2041 ctcccgcttg gaccagtgtg ccctgcgctg ccggcgctgg gtctctacct cgaagccgaa
     2101 gcctgacgat gcgccggttt cgttgaccac cctgaagccg tcctgattgg ggggcaatgt
     2161 cagaaaggcc tgggtcggga cgccctgggc catgtactcg atgcgcgcct caatctccca
     2221 gacgttctgc tgctcgccag gcagccaggg tatccggtac tcggcgtgcc ggaacgcggc
     2281 cgtgccgacg ccggcgatgc acaggagggc caccaggaag agaaatggcc ccttttgact
     2341 catctggagg ttgacggggg ctggtagggc ggctggacga actgcttgcc gacgtccacc
     2401 agcgccatgt cggcgaggaa gttacgtccc agcagcagct gaaaatctag gtgcgaccgg
     2461 tccgccaggg tgaactcgaa ggagtcgtgc acgtcgccga tacgcaagcg catggtgacc
     2521 accggtcggc gggtgccttc gggatcggcc tgctgataga cgcgaacgta gcggaccacc
     2581 cggcgttcca gagtcttgat ctcgtcgtcc agaatcaggt cgaagcgcac ccattcctcg
     2641 ccgtcgcgct cgaattccac caggttctct gcatgcaggg agctggaatg ggcaccggtg
     2701 tcgacacggg cgatgagggt cgtgccgggc ggctccagcc aaacccgctc catctcgccg
     2761 accaccatct tgtcgccgct catgaccacg gtctggatgg gctggtcgga atcgcaggcc
     2821 gccgccgcgg cggtctccgc gctggcgcag atcgcgccga cggcctcggg cacgcggcca
     2881 agctcagtgt tgaggtcgcc catctgcctg gagagatcgg cccggcccga ctgctgatcc
     2941 tcggccagct ccctgaccgc gacctgggtc gccagcacct ggtactccag cgcctgcacg
     3001 cggctgacca gcgccgcgtt gggattatcc tggctgccgg tggtgactgc gcatccgctc
     3061 atcagcagcg ccagcagcgg cgcccgcagc agggcgcggc ccggaccggc gcggtaagcc
     3121 atagcgccgc gccgaacgtc ttcaaaggcc gttctcggtc ggttcatctg cagtcagttt
     3181 gggcttcggc gtcgaagtct actgtcggta tccgacgctt gatactgtac atatatacag
     3241 ttattctgcc tgtctgttgg aaggcccttc aatggcagag agaaatcgcg cgcagaatcc
     3301 gccgccgccc agccggtcgg acaacggctg gttgaagggc cgcggcgcta ccaaaaacga
     3361 ggcgagccgc tatctcgccc ggcacagcgt gcccgtggac gacggctggt cggccaacga
     3421 agtcgatgag ccagacgggt ccagggtggc cacggagctc tatccggaca gaacagtaac
     3481 gctgatcacc cgcaacgcct cgccggacat ccccttcgac cgttccatca atccctacaa
     3541 gggctgtgag cacggctgcg tctattgctt cgcccggccc actcacgcct acctggatct
     3601 ctcgccgggg ctggatttcg agacgaagat cttcttcaag acgaacgtcc gcgagcggct
     3661 cgtcgacgag ctgggaaagc gccgctacca gtgccgcacg atcgccatgg ggaccaacac
     3721 ggatccgtac cagcccgccg agcggcaccg gcggatcacc cggagcgtgc tggagacgct
     3781 gctggaagcc cggcatccgg tgagcatcgt gaccaagagc cagctcatac tgcgcgacct
     3841 cgacctcctt caggaactgg ctcgccaaca gctggcaacc gtggcggtga gcctcacgac
     3901 gctgagcaac gatctgaaga cccgccttga gccgcgcacg gcggggcctg gggccaggct
     3961 cagggtcatc agggagctgc ggggcgcagg cgttcccgtc ggggtcatgg tggcgcccgt
     4021 catccccttt gttaacgatc acgagatcga ggacatcgtg gccgcggcta ccgatgccgg
     4081 tgctcgcacg gcgaattaca tcctgctgcg cctgccgctg gaggtgagcg gcctcttcga
     4141 agcctggctg ggcgtgcact ttccggatcg cgccgagcgt gtcatgaacg cgatacgctc
     4201 ggcccgaggc ggcaagacct acgacgccac gtggggcaag cgaatgcggg gcgagggaga
     4261 agttgccgag ctcatcgccg cgcgcttcaa ggtcgcgctc aggcgcaatg gcattctcgg
     4321 cgccacccag ccgcctcttc gcacagatct cttccgaccc cccggaggca gccagctgag
     4381 cctgatctga taagctttaa ccgctcggcg gggagggtga gcatggatct gatcggctgg
     4441 tcatggttat ttctggtgct ctacctggcg ctgatgctcg ccttcggctt catcggcaga
     4501 aggcggatca aaagcgccga cgacttcgcg accgcgcggg aatcctacgg gcccgtgttt
     4561 ctcgctttcg cctttgcggc caccacggca agcggcgcga ccttcgtcgg cttcccgggt
     4621 atcgcctatg acgcggggct gcctgccgta tggtccgtct ttctttaccc cgcaggcgtc
     4681 tacctcgggg tcctgatctg cctcaaggtc gtcagcaaca ccgggcacga gttcggcagc
     4741 agaagcattc cggagtacct cggcacccga taccagtcgg actggatgcg ggttctggtc
     4801 tctctatttt ccctgctgct cttcttctac ctggcaggtc agctggtctc cgggatcgtg
     4861 atgttcgaga tcatgctcgg cgtctccccg gactgggcgc tggcgatcac cgcggcggtg
     4921 ctgatggtct acgtcgtgct gggcggggcc catgccgaca tcctcacgga cggggtgcag
     4981 ggactgatca tggtggccat cgcggtgggg ctcgtcttcc tcttcgtctc gggcttcgga
     5041 gtcgacggcg gcttcacggg catgattgac agcctcgagc gccaggatgc gaacctggtg
     5101 gggtggctga acccggacta cgtcctctat cactcctggt ggtctgtcgc tgcggtgctg
     5161 ctcgcccaca tccccttggg catgctgccg catatcggca acaagctgtg ggcgctggag
     5221 cagcccagcc agcgccgccg attcatccgg ctggcattca ccttcggcct cgcgctgggg
     5281 atgctggggc tgggcggcct gctcgcgcgg gcaattctgg gagacgccct gctgcagccc
     5341 ggcgagtcga gcaacacggc gctgtccatg ctcttcgtgg agctttttcc gggatggctc
     5401 gccgccctgc tgggtatcgg catcctcgcg gcggtgatgt ccacggccga cggcctggtg
     5461 gtttcgtcgt cgcagatcat cgccaacgac ctttaccgct gcaccatcgt tccccggttt
     5521 gcgcgcgagc tgcccgagcg cactgtggac aggcaggttc tcatcatcag ccgcatagcg
     5581 accgtggcgg taatggccat ctgcaccgcc atggcctggg ccctggccga cagaaacgtt
     5641 gccctgatcg tgtggatcgg aacggggggc atgatggcgg cattcgcggg cccgctggtg
     5701 gttggggccg tctggcgcgg cgtgaccaag gcgggcgcgt tcgccggtct ggtgggcggg
     5761 gtaaccgttt tctccattgc ccacggcggc ctcgtcgacc ccggctggtt cgagcccggg
     5821 gcgctgcgcg atgccgccgc ctggcttctc cgggaagcgc ccaacccctg gtcctgcgcg
     5881 gccatggggg agatcgtgtc catcgcgctg acctggggcg tgtccaagct cacccaaccc
     5941 ctgccgtcag atcatctaac gcggatgttt ggccccgtag gccaggaata gcaccgcgcc
     6001 gacaatggtc caggcgagca cgatggccca ctcgtagggc cagatgagcg cagacgggct
     6061 gccgggcagg taggcggtga gcaggccgat ggcgagcacg aaggcgccga cacccaccag
     6121 gcgcggatgg gagaccttga acggccgggg caggtcgggc tcctggcgtc gcagggccag
     6181 aaaggccagg ggcacgaaca ggtaagccac caccacggcg aagctccccg catcgatgag
     6241 ccagaccagc acggtgcgtc cgaatagagg cgcaaagcag cacatcacgc cgatggtgat
     6301 gaccccgacg tagggcgtcc ggtagcgggg atgcagccgg gcgaatatgg ccgggatgga
     6361 gcctgactcc gccagcgcga acatcacgcg gctgcccccg atcacgaagg cgttccagct
     6421 ggtgaggatg ccggccacgc cgccgagaac gaggaggcgc ccggcgatgg ggtcacccca
     6481 gagagcggtg gcggcgtcgc ccgtggccat ggagctcgtg gccagctcgg gtttgcttag
     6541 cgccgtggcg acggcgaagg cgatcagccc gtaccaggct actgcgagga gcacggatac
     6601 gatgagcagc ttgccgatgc gggcgggttg gaggttgatt tcctcggcgg actggggaat
     6661 cacgtcaaag cccaccagca tggcgggcac catgatcagc acgctgagaa tcccggtggc
     6721 cggcgtggcg atccaaggcc tggcattgtc ggtctccccg aagaccaccg caccgctgac
     6781 gagaatcacg ccggccagca ggatcagccc gatggccgcg ctctgcagcc gcgcggcggc
     6841 cttgatgccc agcacgttga ccacggtgac cagaagcgcg ctgcccgcgc ccacgatcac
     6901 gaagccgacg tcaacgtccg caccgagaac gctccagagg gtacccatgc gaataccggg
     6961 cagcagatac tccaccgccg tgggcaaagc gatggattcg aacaggcaca cgttcacgta
     7021 ggcaaacagg agggcccagg tgcaaacgaa cgaccagcgg tgtcccagcg cgcgatgggt
     7081 gtagacgtgc tcgccgcccg cgcgcggcaa ggcggaggcg agctcgctgt aggtcagccc
     7141 gatcagcgcg atggccagac cgcccgcggc gaaggcgatg agcgtgccca ggctgccggc
     7201 tgtctgcacc cagaacccgg tcatcagaac ccagctccag ccgatcatgg cgcccagcgc
     7261 cagggtgatg acttcccggc tcttcagcac tcgggcaaag cggggttggt cagggcttgc
     7321 tggcatcgcg tggcgcctgc gggcagaatt cgagcccgca agcttagcgg taattgccgc
     7381 ctccctcgac ctttcgattt caacacctgg agcctggttt gccagacgtc gtcgcattga
     7441 gccatcgcgg caaggagttc gagatccgcc tggcggacga cggcgccctg gaactccatg
     7501 tcgacgggtg cctgcgcaag cgtcgcgagc cgacgggccg ggagcctcag tacgtctgga
     7561 ccaacgtcga gctccagtgg gaggagcacc attacataga ggcccgttac tgggcaggga
     7621 accgacgcct gaaggtgacg gtcaatggtg acgggattta cgaccgatcg ctgtcttgag
     7681 ggatgagcgc cgcctactgc agacgggccg atgaggcgcg tcgagatcga gattgaaggc
     7741 gagtatgatg gccggtcctc gaaacgccga gattacgaac tgggagcaga catgcaggac
     7801 atgacccttg agcagatgct ggcgcttctc gagcgccagc gagaggatta ccttggggaa
     7861 ggaaccgttt cgttggccaa gcgcgtggat cgcctcgagc gggctgtggg tctcctcaag
     7921 acgcacgaga agcggctggt agaggccatg acggcggact tcggccatcg cagcgagcat
     7981 cagtcgatgc ttaccgacgt tgccggctcc atcggaccgc tgcggcatgc tcagaagcac
     8041 ctggcccgct ggatgcgccc ggagcgccgc aaggttggac cgttcccgct gaacgtgctc
     8101 ggcgcgaagg cgagggtgga gtaccagccc ctcggcgtgg tgggcgtcat cagcccgtgg
     8161 aatttcccgg tcaacctgac gtttacgccc ctggccggca tcctcggcgc cggcaaccgg
     8221 tgcatgatca agccgtccga gtacacgccg gccacctcgg aggttatggc agagatcttc
     8281 ccgacggcct tcgacgagct ggagatagcg gtggtcaccg gtggccctca ggtgggcgcg
     8341 gatttctcgg ggctgccgtt cgatcatctt ctgttcacgg gagccacatc catcgccagg
     8401 cacgtgatgc gggcggcgtc ggaaaacctg gtgcccgtga ccctggagct cgggggcaaa
     8461 tcgccggtgg tggtcgggcg ctctgccgac atccagaaaa ccacggatgc gatcatgacc
     8521 ggcaagatga tgaacgcggg acagatctgc ctcgctccgg attacgtctt cgtgcccgaa
     8581 gagcgggtcg atgagttcgt cgagtcctcg gcgcgttccg tcgagaagat gtacccgacg
     8641 ctgctggaca atccggacta cacgtcggtg atcaatcagc gtcactacga ccgcctccag
     8701 ggctacgtgg aggaggctcg ggagaaaggc gccaaggtag tagaggtcaa cccggccaac
     8761 gaggacttcc gtcagcaacc gcatcacaag atcccgccga cgctgatcgt cgacccggac
     8821 gagtcgctgc aggtcatgca ggacgagatc ttcgggccga ttcttccggt caaagggtac
     8881 gccaacgttg ccgacaccat cgactacatc aaccaccatc cccggccgct gggactgtac
     8941 tacttcggcg acgaccgcga agaggaagga aaggtccttt cgcacaccac ctcgggcggg
     9001 gtgacggtga acgacgtggt tatgcacgtg gctcaggagg atctgccgtt cggcggggtg
     9061 gggccgtcgg gcatgggcgc ctaccatggg cacgacggct tcaagacatt cagccacgcc
     9121 aagtcgatct tcacccagag caaggtcaac gtggccgagc tggccggcct gcgtccgccc
     9181 tacggcgaga agatgctcaa ggccatccgc gcccagatgc gttgagcgta ccttggaatc
     9241 cggcagcagc gagatcgacc gcctcgagcc gcgcgctttt gagcgcgacg gcttcgtcgt
     9301 ggtgcgcggc tgctgcgacc cggggctggt gaggcggatt cgcgatcgcg cctcttccta
     9361 tctccagccc ctgctggggc ccgcagagtt cgaagctgac gtggggtatc ccggatcccc
     9421 tgcgagccgc gcttcggatg ggggcgatac gtgccgccgt ctcctgcatg cctacgctcg
     9481 agattctgtt ttcagggact gggccacagg aagctccgtg ggggccgttc tccgcgttct
     9541 tttcgggggc gcggacgtga tgatgtcgca atgtcatcac aactgcgtga tgaccaagca
     9601 tcccggctac agcagcgcga cgctgtggca tcaggacatc cggtactggt cgttcgaccg
     9661 ccccgagctc gtctccgtct ggctcgcgct gggcgaagag acggaggaaa acggcgcgct
     9721 caaggtcatt ccgggcagcc acgccatgaa catcgatcgg ggccggctcg accgggatct
     9781 tttcctgcgc cccgagctcg ccgagaacgc cgagctcgtt gagcatgccc agacggtccc
     9841 gcttgaagca ggcgacgtgc tgttttttca ctgccggttg ttccacgcgg caggcaagaa
     9901 tctcacggac gaggtgaagc tttccgtggt gaccacgtac cacaccgccg acaaccggcc
     9961 gatcccggat acccggtctg cgcagtatcc gggagtgccg ctgtagccga atcatgggcg
    10021 acgaggacgt ctttcgcgag gccatgagcg acgttgcgcc gctgcagcgc aagcagcgcg
    10081 cgcaacccga gcgagggccg gtcgaggcga cctccgcgca gctcgaacgc cgcgaattcg
    10141 ccctcggcaa ccgggaccgc gaggccgcgg acccgaatta cctgaccctt ggcgaggtgc
    10201 cccagcggca gccgcgggag attgtcgagt ggaagaagga cggtgtgcag aacgccgtct
    10261 tcgcgcggct gaaggcggga aagtatccgg tggagggttc tgtggatctg caccggcaaa
    10321 cggtgaggga agcccgagcc gccgtgttcc ggtttctcaa tctcgccatg gcgaagggct
    10381 ggcgcatggt gctcatcgcc cacggccgcg gcgagcagag cgagaccccg gcgcgtatca
    10441 aaagctacgt ggccgcctgg cttgctcagg tgcccgaggt gatcgcctat cacagcgccc
    10501 agaacaacca gggcggtacc ggcgcctgct acgtgctgct gaagaagtcc cctcaggccc
    10561 gtgaaaataa tcgcgagcaa tatggcggca agggg
//
LOCUS       DBNHKV010000085        13272 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_13072, whole genome shotgun sequence.
ACCESSION   DBNHKV010000085 DBNHKV010000000
VERSION     DBNHKV010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13272)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13272)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13272
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_13072"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="AC16PP_02695"
     CDS             complement(<1..240)
                     /locus_tag="AC16PP_02695"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6480561.1"
                     /translation="MKVDGRCFCGAVTYDAEIDPDRVLICHCTDCQNHSGSAYRVVAG
                     VVNDSFRLLTGELRSYEKVADSGSLRSRTFCPECGT"
     gene            413..3091
                     /gene="acnA"
                     /locus_tag="AC16PP_02700"
     CDS             413..3091
                     /gene="acnA"
                     /locus_tag="AC16PP_02700"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR6480562.1"
                     /translation="MSLDTFKTRRTLTVGGREYDYFSLPEAQSAGAGNVEKLPMSLKI
                     LLENLLRFEDDNTVKVEDITALANWASGGAAAEEIAYRPARVLMQDFTGVPAVADLAA
                     MRSAVVSAGLDPTIINPLSPVDLVIDHSVMVDSFGTETAFAENVALEMQRNTERYRFL
                     RWGQKAFDNFRVVPPGTGICHQVNLEYLARTVWTREENGRTVAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPISMLIPEVIGFRLTGKLRDGITATDLVLTVVEMLR
                     RRGVVGKFVEFFGEGLDQLPLADRATIANMAPEYGATCGYFPIDRETIAYLELSGRPA
                     ETVELVEAYARANAMWRDADTPEATYTDVLALDLDSVVPSLAGPKRPQDRVAMKDLGR
                     VFDETLELQGRAGDSDARVPVPGTDYDLGHGDVVIAAITSCTNTSNPAVMLGAGLVAR
                     KALARGMQVKPWVKTSLAPGSKVVTEYLEKTAVQEDLDALGFNLVGYGCTTCIGNSGP
                     LPEHISAAIKQGDLVVSSVLSGNRNFEGRVHQEVRANWLASPPLVVAYAIAGTTRIDL
                     TRDPIALDDQGREVFLRDIWPSNEEIADLVAQVSAEMFERQYADVFTGPDAWRAIDVA
                     ESGTYGWENSTYIKQPPFFTVGGDLDQPAEVSDARILALLGDSVTTDHISPAGAIQPD
                     SPAGQYLQQHAVDVADFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGIEGGYTRHVPS
                     GDQVSIYDAAIRYQGEGTPLVVIAGKEYGTGSSRDWAAKGTRLLGVKAVIAESFERIH
                     RSNLIGMGVLPLQFREGEGRASLNLSGEEIVDVRLPDGVTEISPRQDIEVVIKRGTDT
                     RRVTVQSRIDTENEIAYFKSGGILQYVLNNLVREAG"
     gene            complement(3156..4988)
                     /locus_tag="AC16PP_02705"
     CDS             complement(3156..4988)
                     /locus_tag="AC16PP_02705"
                     /inference="COORDINATES: protein motif:HMM:NF015557.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR6480563.1"
                     /translation="MPEVAVESSLHAINAHAVAVMLLTVSALYLFTRERIPMEVTSVG
                     LLAILAVGFSIFPFPGIEPTDFFHGLGHEALVAVCALMVVGQGLVRTGALEPVGRLLG
                     KIWSRAPFLSFLITLIVGAVLSAFVNNTPIVVLLLPILISVCLRTSSSPSGILLPMGF
                     ATLVGGMATTIGTSTNLLVVSVANDLGLERFGMFDFALPAAIAGCVALLYLWLIAPRM
                     LPKRTIGLEDSAPRLFEAGLHLGDNSPAVDRTLAEAKAMTGGDMQVIRIRRGDTYLVP
                     LPDANLRAGDILRVRDTPQRLKSYEDVLKARLYSGDHEVDEDHPLSAGNQMLAEVAVV
                     RGSTLDRTNLRFARFLSRYQLAALALHRAGKSVERPIEEIEDVILQQGDILLVQGARD
                     QIAALKRSTEFLVLDASVALPHTSKAPLALTIVAGVVAAAAFGIMPIAVGAMAGAALL
                     LLTRCLNLGAAIRAISPSVFFIVVASLALGQALIATGATDYVTSLFLSATRGASPTVI
                     LSALMLLLAVLTNMISNNAAAVIGTPIGIGIAAQLGLPAEPFVLAVLFGANMSYATPM
                     AYKTNLLVMSAGNYSFSDFVRVGVPLTIIMWLTLTWVLSSMYGL"
     gene            complement(5120..7288)
                     /locus_tag="AC16PP_02710"
     CDS             complement(5120..7288)
                     /locus_tag="AC16PP_02710"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HIR6480564.1"
                     /translation="MRKSSRTLFRSRSVMLAGTGLCIVAAWLASIAAAENPQLALSTP
                     TETADLLSPLDVHPRISLTIVEQLRHNHYLEKELDDQQSSHIFENYLGMLDGAKVYFT
                     AADVAAMERYRYKLDDALVRGNLVPAFEIFNRYQERLTDRLEFLLEELALGLKQIDFT
                     AEDQIEIERENAPWPANEAELDDLWRKRLKAAVLSMKLNGKELDEIQTLLTKRYTNRL
                     KQSRQTKSEDAFQLYVNAFATTYDPHTQYFSPRTSQNFNINMSLSLEGIGAVLRSEDE
                     YTSIVELVPAGPADKSGLVKPADRIVSVGQGDTGPLIDVVGWRLDDVVELIRGPKGSQ
                     VRLEIIPGDSEDDTSRVISITRNTVKLEEQAAQKKLLTMERNGQTQKIGVIEIPTFYV
                     DFKAIQQGDPNYKSTTRDVRLLIEELKAEGIDGLVVDLRNNGGGALQEADSLTGLFIK
                     SGPTVQVKSASRRPLVYSDDDNRAVWDGPLAVLVNRLSASASEIFAGAIQDYERGIII
                     GSQTFGKGTVQTLVPLNRGQLKITAAKYYRVSGQSTQHQGIIPDIEFPEIYDAERIGE
                     SALDDAMPWDMIQPAVYPRSHQIQPVLAELNSKHEERAGGDPDFQYFEALAQRSRENN
                     RRTHVSLNEKERVVQKKDEDTWRLDLENQLRVAKGEEPLESIDDLDDREADEDEAEDP
                     FGDPMIRESANILLDYIGLTRQIAYVEDLDKAAGPATAIQ"
     gene            7680..8357
                     /gene="bamJ"
                     /locus_tag="AC16PP_02715"
     CDS             7680..8357
                     /gene="bamJ"
                     /locus_tag="AC16PP_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BamJ family beta-barrel-assembly machinery
                     protein"
                     /protein_id="HIR6480565.1"
                     /translation="MGCQPAGDGTAADAQAAEESAELALDDQVAQASYGIGYTIASNL
                     DSQFAGSLNTEALMAGLQDSMEGRERRLSEAAIQTAIAALAQKQQEAVAARSADVLAA
                     GRAFLEENGRRDGVSTTASGLQYEIIATGDGPKPGASDTVSTHYHGTLIDGSVFDSSV
                     ERGQPASFAVNRVIPGWTEALQLMSVGSKWRLFIPPELAYGDRAAGSIPPNSTLIFEV
                     ELLAVDG"
     gene            8405..8674
                     /locus_tag="AC16PP_02720"
     CDS             8405..8674
                     /locus_tag="AC16PP_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480566.1"
                     /translation="MSGERRRPELGNTLTKRRMEPRRRGRLARTILLGTVAVAFSLFW
                     VARELELDTEELLGFLGASAVFVGLMVILGLVGAAAVLIFKRLGR"
     gene            complement(8690..9373)
                     /gene="lepB"
                     /locus_tag="AC16PP_02725"
     CDS             complement(8690..9373)
                     /gene="lepB"
                     /locus_tag="AC16PP_02725"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR6480567.1"
                     /translation="MTGRLHEHWVTWRGFALFLAIMLVFRSAVADWNQVPTASMFPSI
                     FVGDRIVIDKLAYGLRIPFTHQSITSWDDPHRGDVITFTSPEDERLLIKRVVGIPGDT
                     VALDGNRLVVNGQTARYTELTATERRALDGVADREYRLLRESLLGSQRLVMVRSSRVQ
                     DDDLPPTVVPPGQYLVLGDNRDNSRDSRSIGLVARDRILGKATAVAFSLNYDRYYAPR
                     VDRFFADLP"
     gene            complement(9382..9678)
                     /locus_tag="AC16PP_02730"
     CDS             complement(9382..9678)
                     /locus_tag="AC16PP_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HIR6480568.1"
                     /translation="MDKDQENAIEAAVFRRLLEHLDEHKDVQNIDLMNLAGFCRNCLS
                     KWYRAACEDAGVAMDYEAARERVYGMPYADWKSRYQAEATPEQLAGFESGRQQG"
     gene            9795..10574
                     /locus_tag="AC16PP_02735"
     CDS             9795..10574
                     /locus_tag="AC16PP_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR6480569.1"
                     /translation="MELLTNPEIWAAFVTLTILEIVLGIDNIIFISILTSRLPPEQRA
                     RGRVLGLGLAMLMRILLLLSLAGIMRLTTDLFEVMGQGISGRDLILIFGGAFLMAKAT
                     REVHNALEGEAHEGQGAGAPTFAGVLLQIALIDMVFSLDSVITAVGLVDEVPVMVAAV
                     ISAVLVMMVAAGPISDFVEDHPTVKMLALAFLLLIGLTLMGEGIEIHTPKGYIYFAMA
                     FSFGVEILNIRAKKRRKDHVMLRKTQLADLVASRSEKGRPD"
     gene            complement(10587..11363)
                     /gene="yaaA"
                     /locus_tag="AC16PP_02740"
     CDS             complement(10587..11363)
                     /gene="yaaA"
                     /locus_tag="AC16PP_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HIR6480570.1"
                     /translation="MLAVLSPAKTLDFETPSPTRKHSLPVFLDESKQLVETLRTQSPA
                     DLAQLMSMSTKLADQNYQRYTDWQTPFTAGNAKQAALAFKGDVYLGLEPGTFSERDFS
                     WAQKHIRILSGLHGILKPLDLIQPYRLEMGTRLKTRRGADLYAFWGQKITDELNAALA
                     EQKRPVLINLASNEYFDAVDPGRLNARIVTPTFKDLKNGRYRFMSFFAKRARGMMAGY
                     LVKNRVNTLKGLREFDWGGYYYSEDLSRGDDWVFLRDKPA"
     gene            11415..12134
                     /locus_tag="AC16PP_02745"
     CDS             11415..12134
                     /locus_tag="AC16PP_02745"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR6480571.1"
                     /translation="MRIIGRTDRGRVRGSNQDAWACDAELGVAVLADGMGGLTGGEVA
                     SRAAVECVLGHLAGVPCIDADSVREAIAAANGRVLELSREQADGDMGTTLVVWARQAP
                     GRYLLGHVGDSRAYRLSGAVLEPLTRDHSVVQAMVDDGRLSEADALCAPDRNLITRAV
                     GLEVDVFADVVELEYEAEDAFLLCSDGLTDLLAPDELAEICDGHLGPAMRDLPDSLVN
                     AANAAGGYDNITVVLVYNCDP"
     gene            12154..12468
                     /locus_tag="AC16PP_02750"
     CDS             12154..12468
                     /locus_tag="AC16PP_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HIR6480572.1"
                     /translation="MSIQDRINERLQSRFDLHHLEVVNESGGHNVPPGSETHFKVILV
                     SPQFEGLRLIQRHRLVHETLAEELASGVHALAVHTYTDAEWRSRFGSAPMSPPCEGGG
                     RA"
     gene            12465..>13272
                     /gene="trhO"
                     /locus_tag="AC16PP_02755"
                     /gene_synonym="yceA"
     CDS             12465..>13272
                     /gene="trhO"
                     /locus_tag="AC16PP_02755"
                     /gene_synonym="yceA"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF001133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent tRNA uridine(34) hydroxylase
                     TrhO"
                     /protein_id="HIR6480573.1"
                     /translation="MTSVVTFYKFVRLLDPSGLRDELDGMACELALKGTILLAGEGIN
                     GTVTGNAVALQKFLGALERLPEFSGLAVKYSSAAAGNPVFHRLKVRVKPEIVSFGVSG
                     LDPGGRTGTHVGAEEWNELLDDPEVTVVDTRNDYEVAIGSFPGAVNPATQSFRQFADF
                     VADHLDPDRDTRVAMFCTGGIRCEKASAYLLERGFAEVYQLDGGILNYLETVKGEANR
                     WEGECFVFDQRVSVDRHLREGSYEQCFACRRPLSTDDLESPDYRPGISCPH"
BASE COUNT         2294 a         4107 c         4446 g         2425 t
ORIGIN      
        1 ggtaccgcac tcggggcaga aggtcctcga gcggagcgag ccgctgtcgg caaccttctc
       61 gtacgatctc agctcgccgg tcagcaaccg gaagctgtcg ttcaccactc cggccacgac
      121 acggtaggcg ctgcccgagt gattctggca gtcggtgcag tggcagatga ggacccggtc
      181 ggggtcgatc tcggcgtcgt aggtcacagc gccgcagaag cagcgtccgt cgactttcat
      241 gaaggcatcc tcccaggcga atgtcccgcc aagactacca gagctgcgcg ctgcgggcgt
      301 atccacgggt ttacgccccg cttcgcgccc tacaaaacac tgctcaggag ccgggtaacg
      361 agtacaatac cgcccctttt tcctggcacc gaaaacaacg agcgaggcag ccatgagctt
      421 agataccttc aagacccgca ggacgctcac cgtcggcggt agggaatatg actatttcag
      481 cctgccggaa gcgcagtccg ccggggctgg gaacgtcgaa aagctcccga tgtcgctgaa
      541 gatcctccta gaaaacctgc tcaggttcga ggacgacaat accgtcaagg tggaggacat
      601 cacggcgctg gccaactggg caagcggcgg ggcggcggcc gaggagatcg cctatcgccc
      661 ggcgcgggtg ctcatgcagg acttcacagg tgtgccggcg gtggcggatc tggccgccat
      721 gcgcagcgcg gtggtgtccg ccggcctgga tcccacgatc atcaatccgc tgtctcccgt
      781 ggacctggtc atcgatcact ccgtgatggt cgacagcttc ggaaccgaga cggcgtttgc
      841 ggagaacgtc gccctggaaa tgcagcggaa caccgagcga taccggttcc tgcgctgggg
      901 ccagaaggcg ttcgacaatt ttcgcgtggt tccgccgggc accggcatct gtcaccaggt
      961 gaacctggaa tatctcgcca gaaccgtctg gacccgggaa gagaacgggc gcaccgtggc
     1021 ctaccccgac actctggtcg gcaccgacag ccacacgacg atgatcaacg gcctcggcgt
     1081 gctgggttgg ggcgttggcg gtatcgaggc ggaggccgcc atgctggggc agccgatctc
     1141 catgctgatt cccgaggtga tcgggttccg gctcaccggc aagctgcgcg acggaatcac
     1201 ggccaccgac ctcgtgctca ccgtggtcga gatgctccgc cgcaggggcg tcgtgggcaa
     1261 attcgtcgag ttcttcggag agggcttgga ccagcttccg ctcgcggacc gggcgaccat
     1321 cgccaacatg gcccccgagt atggcgccac ctgcggctat ttccccatcg atcgggagac
     1381 catcgcctat ctcgagcttt cgggtcggcc ggcggaaacg gtggagctcg tcgaggcgta
     1441 cgcacgggcc aacgcgatgt ggcgggatgc cgacaccccg gaagccacct ataccgacgt
     1501 tcttgccctg gatctggact ccgtggtgcc gagcctggcg gggcccaagc gcccccagga
     1561 tcgtgtggcc atgaaggatc tgggcagggt ttttgacgaa acgctggagc ttcagggccg
     1621 cgcgggtgac tccgacgcac gtgttccggt cccgggcacg gactacgacc tcggccacgg
     1681 ggacgtggtg atcgccgcca tcacctcctg cacgaacacg tcaaaccccg cggtcatgct
     1741 gggtgccggc ctggtagcgc gcaaggcgct cgctcgcggc atgcaggtca agccctgggt
     1801 gaagacgtcg ctggcgccgg gttcaaaggt cgttaccgag tatctggaga agacggccgt
     1861 ccaggaagat ctggatgccc tcgggttcaa cctcgtgggc tatgggtgca cgacgtgcat
     1921 cggcaattcc gggcctctgc cggagcacat ctcggccgcc ataaagcaag gggatctggt
     1981 ggtgagctcg gtgctgtcgg gtaatcgcaa cttcgagggt cgcgtccatc aggaggtcag
     2041 ggccaactgg ctggcttcgc ccccgctggt ggtggcctac gccatcgctg gcacgacgcg
     2101 catcgatttg acccgcgacc cgatcgccct ggacgaccag ggcagagagg tgtttctccg
     2161 ggacatctgg ccgagcaatg aggaaattgc cgatttggtg gcccaggtct ccgcggagat
     2221 gttcgagcgt cagtacgcgg acgtgttcac cggtcccgat gcctggcgtg ccatcgacgt
     2281 ggcggaatcg ggcacgtacg gctgggaaaa ctccacgtac atcaagcagc cgccattctt
     2341 cacggtgggc ggtgatctcg atcagccggc cgaggtgtcg gacgcccgta ttctggcgct
     2401 gctcggcgac tcggtgacca ccgaccacat ctcgccggcg ggcgccattc agccggacag
     2461 cccggcgggt cagtacctgc agcagcacgc cgttgacgtg gcggacttca actcctacgg
     2521 ctcgcgtcgc ggcaatcacg aggtgatgat gcgcggtacc ttcgcgaaca tccggatccg
     2581 caacgagatg gtccccggga tcgaaggggg ctacaccagg cacgtgccgt caggggacca
     2641 ggtgagcatc tacgatgccg cgatccggta tcagggcgag ggaacgccgc tggtggtcat
     2701 cgccggcaag gagtacggca ccggatcgag ccgggactgg gccgccaagg gcacccggct
     2761 gctgggcgtc aaggccgtga ttgccgagag cttcgagcgc atccaccggt cgaacctcat
     2821 cggcatgggc gtgctgccgc tccagttccg cgagggtgag ggccgcgcgt ccctcaatct
     2881 ctcgggagag gagatcgttg acgttcgtct gccggacggc gtgaccgaga tctcgccgcg
     2941 ccaggacatc gaggtggtga tcaagcgcgg gacggatacc cggcgggtga ccgtgcagag
     3001 caggatcgac acggagaacg agatcgccta cttcaagagc ggcgggatcc ttcagtacgt
     3061 gctgaacaac ctggttcgcg aggcaggctg acggttgctc gaagagcgct ccggccggcg
     3121 gcgcgccgtg cgcgcacggc cccagggcga ggcgctcaca acccgtacat gctgctcaat
     3181 acccaggtga gcgtgagcca catgatgatg gtcagcggta cccccacccg gacgaagtcg
     3241 gagaagctgt agttgccggc gctcatgacg agcaggttgg tcttgtaggc catcggcgtg
     3301 gcatagctca tgttcgcgcc gaagagcacg gcaaggacga agggctccgc cggtagcccc
     3361 agctgcgcgg cgatcccgat cccaatgggc gtgccgatga cggctgccgc gttgttcgag
     3421 atcatgttgg tgagcaccgc cagcagaagc atcagcgcgg acaggatgac cgtcggcgat
     3481 gccccccggg tcgcgctcaa gaaaagcgac gtcacgtagt ctgtcgcccc ggtggcgatg
     3541 agggcctggc cgagcgccag gcttgccacg acgatgaaga acaccgaggg gctgatggct
     3601 cggatggccg cccccagatt cagacaccgc gtgagcagca acagcgccgc acccgccatg
     3661 gcgcccacgg cgatcggcat gatcccgaag gcggccgcgg cgaccacccc ggcgacgatg
     3721 gtcagcgcca gcggggcctt ggacgtgtgc ggcagagcga ccgacgcgtc caggacgaga
     3781 aactcggtgc tgcgctttaa ggccgcgatc tgatcccgtg cgccctggac cagcaggatg
     3841 tctccctgct gaaggatcac gtcctcgatc tcctcgatgg gccgctccac tgattttcca
     3901 gcccggtgca gggcgagcgc ggccagctgg tagcgcgaca ggaagcgggc gaacctcagg
     3961 ttggtgcggt ccagggtcga cccgcgaacc accgcgacct cggccagcat ctggtttccg
     4021 gctgaaagcg ggtggtcttc gtccacctcg tgatcgccgg agtagagccg tgctttcagc
     4081 acgtcctcgt aggacttgag ccgctggggc gtatccctga cccgcagaat gtctcccgcg
     4141 cgcaggttgg cgtcgggcag aggcacgaga tacgtgtcgc cccggcggat tcgaatgacc
     4201 tgcatgtcgc cgccggtcat cgccttcgcc tcggccagcg tgcggtccac cgcgggcgag
     4261 ttgtctccca gatgcagtcc ggcctcgaat agccggggcg ccgagtcctc caggccgatg
     4321 gtccgcttcg gcagcattcg gggggcgatc agccacagat aaagcagggc tacgcagccc
     4381 gcaatggcgg cgggcagcgc gaagtcgaac atcccgaaac gctccaggcc caggtcgttc
     4441 gccacgctga ccaccagcag gttcgtggac gtgccgatgg tcgtcgccat gccgcccacc
     4501 agggtggcga aacccatggg aaggagaatg ccggatggcg acgatgacgt gcgcaggcag
     4561 acgctgatga gaatgggcag cagcagaacc acgatgggcg tgttgttcac gaacgccgac
     4621 agcacggcgc cgacgatgag cgtgatcaga aacgacagga acggagcgcg gctccagatc
     4681 ttgccgagca atcgacccac gggctcgagc gcccctgtcc ggaccaggcc ctggccgacc
     4741 accatcaggg cgcagacggc caccagcgct tcgtggccga gcccgtgaaa gaaatcggtg
     4801 ggctcaatgc ccgggaaggg aaagatggag aatccaactg cgaggatggc gagcagaccg
     4861 acgctggtca cctccatggg tatgcgctcg cgggtgaaga gatagagcgc gctgaccgtg
     4921 agcagcataa cggcgaccgc gtgagcgttg atcgcatgca ggctgctttc tacagccact
     4981 tcaggcatcg tgcgtctgac aagaaagcaa cttagatgac gtccacgaga aggtcggaga
     5041 agtgtacgtc tattctgggg tcaaagcctg cagaaatctc tcaatattgg cccggagcgt
     5101 cgggtcgccc ggcgattttc tactggatgg ccgtcgcggg cccggcagcc ttgtcgaggt
     5161 cttccacgta ggcgatctgc cgggtgaggc cgatgtagtc gagcagaatg ttggcgctct
     5221 cgcgaatcat gggatcgccg aaaggatcct ccgcttcgtc ctcgtccgcc tcccgatcgt
     5281 ccaggtcgtc gatggattcc agcggctctt ctcctttggc gacccggagc tgattctcca
     5341 gatccaggcg ccacgtgtcc tcgtccttct tctgcaccac gcgctctttc tcgttcaggg
     5401 agacgtgggt gcgacggttg ttctcgcggc tgcgctgggc gagggcttcg aagtactgga
     5461 agtcaggatc gccgcccgca cgctcttcgt gcttggagtt gagctccgca aggaccggct
     5521 gaatctgatg tgaccgcggg tacacggcgg gctggatcat gtcccagggc atggcgtcat
     5581 cgagggcgct ttcgccgata cgctccgcgt cgtatatctc cgggaactcg atgtcgggaa
     5641 tgatgccctg gtgctgggtg ctctggccgg acacccgata gtacttggcc gcggtgatct
     5701 tcagctgacc ccggttcaag gggaccaggg tctgcaccgt gcctttgccg aaggtctggc
     5761 tgccgatgat gatgccgcgc tcgtagtcct gtatggcgcc cgcgaagatc tccgaggccg
     5821 aagcggagag acgattgacc agcaccgcga gggggccgtc ccacacggcc cggttgtcgt
     5881 cgtccgaata gaccaggggc cgtcgcgagg cggatttgac ctgcacggtg ggcccggact
     5941 tgatgaacag cccggtgagc gaatccgctt cctgcagggc gccaccgccg ttgttgcgaa
     6001 gatcgacgac gaggccgtct atgccctccg ccttgagctc ctcgatcagc agccgcacgt
     6061 ctcgcgtggt gctcttgtag ttgggatcgc cctgctgaat ggccttgaag tcgacataga
     6121 acgtcgggat ctcgatgacg cctatcttct gggtctggcc gttgcgctcc atggtgagca
     6181 gcttcttctg ggcggcctgc tcctcgagct tcacggtgtt ccgcgtgatg ctgatcacgc
     6241 ggctggtgtc gtcctccgag tcgccgggga tgatctccag cctcacctgg gagcccttgg
     6301 ggccacggat gagctccacg acgtcgtcga gccgccagcc caccacgtcg atcagcggtc
     6361 cggtatcgcc ctggcccacg ctgacgatcc ggtcggccgg cttgaccagc ccggatttgt
     6421 ccgcgggccc ggcggggacg agctcgacga tggacgtgta ctcgtcctcc gagcgcagaa
     6481 cggcgccgat gccttccagg gaaagcgaca tgttgatgtt gaagttctgc gacgttcgcg
     6541 gcgagaagta ctgggtgtgc gggtcgtagg tggtcgcgaa ggcgttcacg tagagctgaa
     6601 acgcatcttc gctcttggtc tgccggctct gcttgagccg gttggtgtag cgcttggtca
     6661 gcagggtctg gatctcgtcg agctctttgc cgttgagctt catgctgagc accgctgctt
     6721 ttagccgctt gcgccacaga tcatccagct cagcctcgtt cgcgggccac ggggcgttct
     6781 cgcgctcgat ctcgatctgg tcctcggcgg tgaagtcgat ctgcttcaga cccagcgcca
     6841 gctcttccag caggaactcc agccggtcgg tcaggcgctc ctggtaccgg ttgaagatct
     6901 cgaaggcggg cacgaggttg ccccgcacca gagcatcgtc cagcttgtag cgatagcgct
     6961 ccatcgccgc cacgtcggcg gcggtgaagt acaccttggc accatccagc atgccgagat
     7021 agttctcgaa gatgtggctc gactgctggt cgtccagttc cttttcgagg tagtggttgt
     7081 gacgcagctg ctcgacgatg gtgaggctga tccgcgggtg aacgtccagc ggcgacagaa
     7141 ggtcggcggt ctctgtcggg gtagacagcg ccagctgcgg attctcagcc gctgcgatgg
     7201 aggcgagcca ggccgcaacg atgcataagc cggtgcccgc cagcatgacc gagcgggacc
     7261 ggaagagggt tctagagctt ttgcgcataa cgtgagtgat ctgatggtgc cctgaatgaa
     7321 aatgtgggtc gcattggcgg cgtcgggtcc aggccctggc cttcgtattg ttcgtaactc
     7381 agtcgccggg gatctgcaga ttttcgccgg tccggtcggc aaactcgctt ctgccacccg
     7441 tttcggtaca aactgtgccg caactgatcg ctacttctgt agataccatt tctcctgggt
     7501 ggttccccca aaccactcca gcgagactat ctaaatgccc gcatgcgtac tgttgcggga
     7561 atgtaacccg gtgtctgggt gtgggcaacc ggggcaggga gcccaaattg atggaatgcc
     7621 gaacggacat gagtacttgg tcaagaaatt tcgcatcttt gctggtggtg ctgctggcga
     7681 tggggtgcca gccggccggg gacggcaccg cggcggatgc gcaggcggca gaggaatccg
     7741 cagagctggc gctggacgat caggtggccc aggccagcta cgggatcggg tacaccatcg
     7801 cgagcaatct ggactcccag tttgcgggct ccctgaacac cgaggccctg atggcgggcc
     7861 ttcaggacag catggagggt cgggagcgcc ggctttccga ggcggcgatc cagaccgcca
     7921 tcgcggcgct ggcacagaag cagcaggagg cggtggccgc tcgctccgcg gatgtgctgg
     7981 ccgccggccg ggcgtttctg gaggaaaacg ggcgccggga cggcgtcagc acgacggcgt
     8041 cgggactgca gtacgagatc atcgcgaccg gcgacggtcc caagccgggc gcctcggaca
     8101 ccgtgagcac gcactatcat ggcaccctga tcgacggcag cgtgttcgac agctccgtgg
     8161 aacgcggcca gccggcaagc tttgccgtca accgggtgat tccgggctgg acggaagcgc
     8221 tccagctgat gtcggtgggt tccaagtggc gtctgttcat tccgcccgag ctcgcatacg
     8281 gagatcgggc agcgggctcc atccctccca actcgacgct gatcttcgag gtcgagctcc
     8341 tggccgttga cggttagcac gcacgtggct catcgcgagc gggtctggac ggcggccgct
     8401 tcccatgagc ggtgagcgcc gccgccctga gctcggcaac acgctgacca agcggcgcat
     8461 ggagccccgc aggcgcgggc gccttgcccg gaccatactg cttggcacgg tggcggtggc
     8521 gttctcgctg ttctgggtcg ctcgggaact ggagctcgac accgaggagc tgctgggttt
     8581 cctgggtgcc agcgccgtgt tcgtcgggtt gatggtgata ctcgggctgg tgggcgcggc
     8641 cgccgtcctg atcttcaagc gcctcggccg ctgacggccc gctcacggat cagggaagat
     8701 cggcaaaaaa gcggtcaacc ctcggcgcgt agtaccgatc gtagttcagg gagaaggcga
     8761 cggccgttgc cttgccgaga atccgatccc gggccaccag accgatgctg cgcgagtccc
     8821 ggctgttgtc tcgattgtcg ccgagcacca ggtattggcc gggaggcacg acggtgggcg
     8881 gcaggtcgtc gtcttgcaca cgggagcttc gcaccatcac cagtcgctgg ctgcccagca
     8941 gagattcccg cagcagccga tactcccggt cggcgacgcc gtccagcgcc cggcgttcgg
     9001 tggccgtcag ctcggtgtag cgggcggtct ggccgttgac gacgagccgg ttcccgtcga
     9061 gcgcgacggt gtcgccgggt atcccgacaa cgcgcttgat cagcagccgc tcgtcttcgg
     9121 gcgaggtgaa ggtgatcacg tcaccgcgat gggggtcatc ccagctggtg atggactggt
     9181 gcgtaaaagg aattctcagc ccgtaggcca gcttgtcgat cacgatgcga tcgccgacga
     9241 agatggaggg aaacatggaa gccgtcggca cctgattcca gtctgccacg gcggagcgaa
     9301 agaccagcat gatggccaga aagagggcaa agcctcgcca ggtgacccag tgctcgtgca
     9361 gtcggccggt cacgctcctg atcagccctg ctgccggccc gattcgaagc cggccagctg
     9421 ctcgggcgtc gcctcggcct ggtagcggga cttccaatcc gcgtagggca taccgtagac
     9481 ccgctcccgc gccgcttcgt agtccatggc gacgccggcg tcctcgcagg cggcccgata
     9541 ccacttcgac aggcagttgc ggcagaaacc ggccaggttc atcaggtcga tgttctgcac
     9601 gtccttgtgc tcgtcgagat gctccagcag gcgccgaaac accgcggcct ctatggcgtt
     9661 ttcctgatct ttgtccacga cggatcccaa agttgacacg cgttggccgc attctaccga
     9721 aaacttgcgg cctgggtgct tcggcagaaa tcctgtgctt caatgcccgc aagtcaaccg
     9781 gctgacggca gattgtggag cttctcacca acccggagat ttgggccgcg ttcgtcacgc
     9841 tgacgatcct cgagatcgtg ctcgggatcg acaacatcat cttcatctcc atactcacca
     9901 gtcgcctgcc gcctgagcag cgtgcccgcg gccgcgtgct cggtcttggc ctggccatgc
     9961 tcatgcgcat tctgctgctg ctctcgcttg ccgggatcat gcggttgacc acggaccttt
    10021 tcgaggtgat gggccagggc atatcgggcc gcgacctgat actgatcttc ggcggcgcct
    10081 tcctcatggc gaaagccacc cgggaagttc acaacgccct ggaaggtgaa gctcacgaag
    10141 gtcagggcgc cggcgcgccg acgttcgcgg gcgttctgtt gcagatcgcc ctgatcgaca
    10201 tggtcttctc gctggattcg gtgatcacgg cggtaggcct ggtggacgag gtgccggtga
    10261 tggttgccgc cgtgatctcc gcggtgctgg tgatgatggt cgccgcggga ccgatcagtg
    10321 atttcgtgga agatcaccca acggtgaaga tgctcgcgct ggcgtttctg cttctcatcg
    10381 ggctcacgct gatgggcgaa ggcatcgaga tccacacgcc caagggatac atctactttg
    10441 ccatggcgtt ttcgttcgga gtggagatcc tcaacatccg ggccaagaaa cgcaggaagg
    10501 atcacgtgat gctgcgcaag acgcagcttg ccgatctggt tgcctcgcgg tcggagaagg
    10561 gccggccgga ttagccggcc gccggctcag gcgggcttgt cacgcagaaa gacccaatcg
    10621 tctccccggg acagatcctc ggaatagtag tagccgcccc agtcgaactc gcgaagccct
    10681 ttgagcgtgt tgaccctgtt cttcaccagg tagccggcca tcattccccg cgcccgcttt
    10741 gcgaagaagg acatgaagcg gtacctgccg ttcttgaggt ccttgaaggt gggcgtgacg
    10801 atccgcgcgt tcaggcggcc tgggtcgacg gcgtcgaaat actcgttgga ggcgaggttg
    10861 atcagcaccg ggcgtttctg ctcggccagg gcggcgttca gctcgtcggt gatcttctga
    10921 ccccagaacg cgtagagatc ggccccgcgg cgggtcttga gccgggtgcc catctccagg
    10981 cggtacggtt gtatgagatc aagtggcttc aggattccat gcaggccgga cagtatgcgg
    11041 atgtgcttct gtgcccagct gaagtcacgc tcggagaacg tgcccggttc caggccgagg
    11101 tacacgtcgc ccttgaacgc cagggcggcc tgcttggcgt tgcccgccgt gaacggagtt
    11161 tgccaatcgg tgtagcgctg gtagttctga tcggcgagct ttgtgctcat gctcatcagc
    11221 tgtgccaggt ctgccgggga ctgagttctg agcgtctcca ccagctgctt tgactcgtcg
    11281 agaaaaactg gcaggctgtg cttgcgcgtg ggggagggcg tctcgaaatc taatgttttt
    11341 gccggggata gaactgccag catttggccg gaggctcctg gattgattcg ggtgccaagt
    11401 gtaacggaat tcgcgtgaga atcatcggac gcacggatcg cggccgggtg cgcggcagca
    11461 accaggacgc ctgggcgtgc gacgcagagc tcggcgtcgc cgtgctcgcc gacggcatgg
    11521 gggggttgac cggcggggag gtggcaagcc gggcggcggt ggagtgcgtc ctgggacacc
    11581 tggccggcgt tccctgcatc gatgccgaca gcgtcaggga ggccatcgcc gcggcgaacg
    11641 gcagagtttt ggaactttcc cgcgagcagg ctgatggcga catgggaacg accttggtgg
    11701 tctgggcccg gcaggcgccc ggccgctacc tgctcggcca cgtgggcgac tcgcgcgcgt
    11761 accggctgag cggcgccgtc ctcgagccgc ttacccgcga tcacagcgtc gtgcaggcca
    11821 tggtggatga tggccggctg agcgaagcgg acgccctgtg cgcgcccgat cgcaatctga
    11881 tcacccgggc ggtcgggctc gaggtggatg tgttcgcgga cgtggtggag ctcgagtacg
    11941 aagcggaaga tgcgtttctg ctctgctccg atggcctcac cgatctgctc gctccggacg
    12001 agctggcgga aatctgcgac ggccatctag ggccggccat gcgggatctt ccggacagcc
    12061 tcgtgaatgc cgcgaacgcg gcggggggat acgacaacat caccgtggtg ctcgtctata
    12121 attgtgaccc ctgatggttt gcaactgctg tcgatgtcga tccaagaccg gataaacgag
    12181 cgtcttcaat cccgctttga tctgcaccac ctcgaggtgg tcaacgaaag cggcgggcac
    12241 aacgtgccgc ccgggtcgga gacccacttc aaggtgattc tcgtctcccc gcagttcgaa
    12301 ggcctgcgcc tgatacagcg ccaccggctg gtccacgaga cgctcgccga ggagttggcc
    12361 tccggcgtgc acgcgctggc cgttcacacc tacaccgacg ccgagtggcg ctcgcgcttc
    12421 ggcagcgcgc ccatgtcgcc gccatgcgag ggcggcgggc gggcgtgacc agcgtcgtca
    12481 cgttctacaa gttcgtgcgc ctcctcgatc cctcggggct gcgcgacgag ctcgatggca
    12541 tggcttgcga gctggcgctg aagggaacga tcctgctcgc cggggaaggc atcaacggaa
    12601 cggtcacggg aaatgccgtg gctctgcaga aatttctggg cgccctcgag cggttgccgg
    12661 aattctccgg cctggcggtc aagtattctt ccgcggctgc gggcaatccg gtgttccacc
    12721 ggctcaaggt tcgcgtcaag ccggagatcg tcagcttcgg cgtttccggc ctcgacccgg
    12781 gtggccggac cggcacccat gtgggtgccg aggaatggaa cgaactgctc gacgaccccg
    12841 aggtgaccgt cgtcgacacg cgcaacgact acgaagttgc gatcggctcc tttcccggtg
    12901 ccgtcaaccc ggcgacgcag agcttccgcc agtttgcgga cttcgtcgcc gaccacctcg
    12961 accccgaccg cgacaccagg gttgccatgt tctgcaccgg tggcattcgt tgcgagaagg
    13021 cctctgccta tctgctcgaa cgtggcttcg ccgaggtata tcaactcgac ggcggcatcc
    13081 tcaattacct ggagacggta aaaggggagg cgaatcgttg ggaaggcgaa tgctttgtgt
    13141 tcgaccagcg cgtctccgtc gaccggcatc ttcgggaggg ctcgtacgag cagtgctttg
    13201 cctgtcggcg gccgttgtcg accgatgacc tggaatcgcc cgactaccgc cccggcattt
    13261 cctgccccca tt
//
LOCUS       DBNHKV010000086         7756 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_651187, whole genome shotgun sequence.
ACCESSION   DBNHKV010000086 DBNHKV010000000
VERSION     DBNHKV010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7756)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_651187"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2067)
                     /locus_tag="AC16PP_02760"
     CDS             complement(<1..2067)
                     /locus_tag="AC16PP_02760"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF018444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR6480574.1"
                     /translation="MKDTLSVRFLAEFVHRRGDLHARLDGRARAEEGIQAQRKLQRGR
                     GPAYERERRVALTREVGGRAHLISGRVDGADLTARLVEEYKTTRADPELAQQKLGSAH
                     WAQVKLYGGMLVEELDGASDSQWTLRLVYCHPDTLATREFDQQLSGHELATFLDETLD
                     SYAGWVAMQLAHERNRNEAIARRDFPFPRYRPHQQAVARRVYRALRDREQLLLEAPTG
                     SGKSMALLFPAAKALAATDHQKIFYLTSRSTGSLAALDACERLDPDGDYLRRVHIVAR
                     ERACPVEGVPCDPALCQYANGYYDRIHGALAELLERPVMNAPRVAEVAEAHRVCPHEL
                     SLDAAVWADVLVGDYNYVFDPMVRLQRFAEDPGIALLVDEAHQLSTRARDMLSLEVTR
                     SAVRQARVEQPPAAILRRVRSVERKLVEIASAQRRSGETSVPRPDPLIRAMARLVDEV
                     AGSDVDIAPYPATRSLLMTCYRWVRGESWYDEACCAHLGDAPDGDYRLRRVHLDPAAY
                     LKGVFDGFGGHVRFSGSVSPLPLYQRLHGTTGAPAERAGSPFDPEQLEVLVVDDVPTY
                     LAARARSLERLAALVETVVSANRGHYLVALPSFAYLDDLAWALAGRESLPDVAVQTPG
                     MPPEERDRFLERFGSGSPACIGLVVMGGVFAESVDFAGRLAGVICVGVGLPPPEPERA
                     RLQAHFA"
     gene            complement(2064..3680)
                     /locus_tag="AC16PP_02765"
     CDS             complement(2064..3680)
                     /locus_tag="AC16PP_02765"
                     /inference="COORDINATES: protein motif:HMM:NF020354.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HIR6480575.1"
                     /translation="MASSIVPQLAANSPPPPDYYAENLRQLITHVHAHYGDVLRREEI
                     GYGRRIVACSVPAQRLYARLLSRRGPWIRIDKLRYREVSDVTAAVGELEEAGLVVRNG
                     RAPADALLGLLSTAERRELFAAIGDAPKARWINQCVARYSDAHVLSRLAGVHPWVSIR
                     DFPTFWLHQLLFFGDTRQDLSAFVLQDLGFIRYEPYELDPRHRQFANRERLDEYLRYL
                     ELSSLSYRLEEQPVLASWLPGAMWTEPASRWAQRSRDKTANRLGRWHERRGEYDQALA
                     SYARSTSHPARERTARILDRLGDAEGVQDVLHAMLSEPRSAEEEDFARRFGPNGRRKR
                     HAHRLERLPDGDGVRVEQLAVDLLTEGGGRGWHLENRFSLGLAGLAFWPVVFADVPGA
                     FVNPFQQGPVDLFWPDFVEQRAGILEVQLDKLAAQGGLASVVMDTWRRKFGVANRLVS
                     WAHFDEDLLEAVLGQLPQGDLLRLADFTLRNLHRLRKGFPDLLVIRAPGDYEFVEVKG
                     PTDQLQPVQRIWLDALEELDLPARVLRFKA"
     gene            3753..4199
                     /locus_tag="AC16PP_02770"
     CDS             3753..4199
                     /locus_tag="AC16PP_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480576.1"
                     /translation="MPAFSYCICTTVDARALQALLEASGQGSYKDEQPWLIAREILEE
                     AASQSARLPILFASGHPSEFSHWGFIESIDVVELHRGTWESRCAFSPLQPVNPIWTSI
                     DSVFLKPTGEQLRREALEGIAHHRYPLNEGELHPYAICETPAFILA"
     gene            complement(4230..4454)
                     /gene="csrA"
                     /locus_tag="AC16PP_02775"
     CDS             complement(4230..4454)
                     /gene="csrA"
                     /locus_tag="AC16PP_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HIR6480577.1"
                     /translation="MLVLTRRAGESIMIDDDIELKVLKIRGSQVHLGIDAPKRSAVHR
                     KEVWLRINGEPKAAGREDKEDSSDPSRGVA"
     gene            complement(4568..5869)
                     /locus_tag="AC16PP_02780"
     CDS             complement(4568..5869)
                     /locus_tag="AC16PP_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014680.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR6480578.1"
                     /translation="MQARASYAAGQLQQAEAGQAERLVLEERLGERERDLVRLEERIT
                     SFENQLNSARSALDERTKELSDRQAAQAAVEARLEESRKGFAEKEAVFKESSEALKQE
                     FELLATKIFDQQGVKHQERLSSVLTPFKEQIVDFRKRVEQVYHTDTKDRASLLNEVRN
                     LQKASERINEEAENLTKALKGDVKVQGNWGEVVLERVLEESGLREGHEYFLQAARRSE
                     RGELKRPDVLIRLPDGKDVVVDAKVSLLAYEEALSAETDGARELALRQHLVSLRAHVK
                     RLGEQDYDQLSDVRSLDFVLLFVPIEAAFTMAMEHDHRLFTEAFEKRIVIVSPTTLMM
                     TLRIIHNVWRFEKQNRNAQDIARRAGALYDKLRGLIEDMDLLGKQLGTVEKTYQAAYG
                     KLASGKGNLVRQVERFRELGAQVKRPLPKSLLDEATDRSDD"
     gene            complement(5947..6762)
                     /locus_tag="AC16PP_02785"
     CDS             complement(5947..6762)
                     /locus_tag="AC16PP_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6480579.1"
                     /translation="MTGYEQILFEAQDSIAVLTLNRPERMNAWTYQMSRELSHAIGVC
                     NEDPAIGAIIITGAGRGFCAGADIEHNFKARLDGNDDRGDDATPWVRLLRQSKPIIAA
                     VNGASVGVGATMILPADIIVAADSAKFAMGFIKMGLVPELASSHFLVQRMGFGRASEM
                     CLTGKMYPASEAHAMGLVDHLVADDALMDKAMDLAQAIAANPGPQLRWVKQLLTENGC
                     ATDLDEVQRLEGKLLQQAFASPEHKEAVAAFLDKRTPDFIGARAAQGEAGGTA"
     gene            complement(6854..7645)
                     /locus_tag="AC16PP_02790"
     CDS             complement(6854..7645)
                     /locus_tag="AC16PP_02790"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR6480580.1"
                     /translation="MRENALLAAWREGRQTIGCWLSMGNAYSAEALGQLGFDYVCIDL
                     QHGLVDYQDLTYMLPAVSRGTSTPLVRVPWNEPYEIMKALDAGAYGVIVPMVNNAEDA
                     ARAVDACRYPPLGSRSFGPIRGALYGGRGYAAQANEQMACIVMVETREGLDKLEEIVT
                     TPGVDGVYIGPADLALALGLPALGDTDVPEHVAEVTRILEVCHRHELPAGIHTSSLDY
                     CQKRLAAGFDFVTLGSDAGFMTRTAAQELAAARGTAEQEREKTGY"
BASE COUNT         1340 a         2623 c         2437 g         1356 t
ORIGIN      
        1 ggcgaaatgc gcctgaagcc gagcccgctc gggctcgggc ggaggcagcc ccacgcccac
       61 gcagatgact ccggccaggc gtccggcaaa gtccacggac tcggcgaaaa cgcctcccat
      121 cacgacgagg ccgatgcagg cgggcgaacc ggatccgaac ctctccagaa atcggtctcg
      181 ctcctccggt ggcattccgg gcgtctgcac tgcgacgtcg ggaagcgatt cccgaccggc
      241 cagggcccag gcaagatcgt ccaggtacgc gaacgacggc agcgcgacca gatagtggcc
      301 gcgattcgcg gaaaccaccg tttccacgag cgctgccagg cgctcgaggc tccgtgccct
      361 cgccgccaag taagtcggca cgtcgtccac cactagcacc tcgagctgct cgggatcgaa
      421 ggggctgccg gcacgttccg ccggcgcccc ggtggtcccg tgaaggcgct ggtagagcgg
      481 cagaggagac acgcttcccg agaagcggac gtggcctccg aaaccgtcga acacgccttt
      541 caggtaggcg gccggatcca ggtgaacccg gcgcaggcgg tagtccccgt ccggcgcgtc
      601 tcccaggtgc gcgcagcatg cctcgtcgta ccaggattca ccacgcaccc atcgataaca
      661 cgtcatcagc agagaccgag tggccggata cggcgcgatg tcgacgtctg atcccgccac
      721 ctcgtcgacg aggcgcgcca tggcgcgaat caacgggtcg ggacggggaa cggacgtttc
      781 gcccgagcgt cgttgggcgc tcgcgatctc gacaagcttg cgctccacgg accgcacgcg
      841 gcgcaggatg gcggccggcg gctgctcgac ccgcgcctgc cgcacggcgc tgcgcgtcac
      901 ctccaatgac agcatgtccc gggcacgcgt gctcagctga tgggcctcgt ccaccagcag
      961 agcgatgccg ggatcctcgg cgaaccgctg cagacgcacc atggggtcga agacgtagtt
     1021 gtagtcaccg accagcacgt ccgcccagac ggccgcatcc agtgacagct cgtggggaca
     1081 cacccgatgg gcttcggcca cctccgccac cctcggtgcg ttcatgacgg gccgctccag
     1141 caactcggcg agcgctccgt ggattctgtc gtaatagccg ttcgcgtact ggcacagggc
     1201 cggatcacag ggcacgccct ccaccggaca ggcgcgttcc cgcgcgacga tgtgcacgcg
     1261 gcgcaggtaa tctccgtccg ggtcgagcct ctcacaagca tccagtgcgg ccaaggaccc
     1321 ggtagagcgg ctggtgagat agaagatctt ctggtgatcg gtagcggcaa gcgctttggc
     1381 tgcgggaaac aggagcgcca tgctcttgcc gctccccgta ggcgcttcca ggaggagctg
     1441 ttcccggtcg cgcaacgccc gatacacccg ccgggccacc gcctgctgat gggggcgata
     1501 gcgggggaac gggaagtcgc gacgcgcgat cgcctcgttc cggttccgct cgtgggcgag
     1561 ctgcatcgcc acccatccgg cgtagctgtc gagggtctca tcgaggaacg tggcgagctc
     1621 atgcccggac aattgctgat cgaactcgcg ggtggcgagc gtgtcgggat ggcagtacac
     1681 cagccgcaac gtccattggc tgtccgatgc gccatcgagt tcctccacca gcatcccgcc
     1741 gtaaagcttg acctgcgccc aatgggcgct tccgagtttt tgctgggcaa gttccgggtc
     1801 ggcccgggtg gtcttgtact cctcgacgag cctggcagtg agatcggcgc cgtccacgcg
     1861 cccggatatc aggtgcgctc ggcccccgac ctcccgcgtg agcgcaacgc gacgttcgcg
     1921 ctcgtatgcc ggcccgcgtc cgcgctgcag cttccgctga gcctgaatcc cctcctccgc
     1981 ccgggcccgc ccatccaggc gagcgtgcag atcaccgcgc cgatgcacga actccgcgag
     2041 aaaacgcact gaaagcgtgt ctttcatgcc ttgaaccgca gcacgcgagc gggcaggtca
     2101 agttcttcca gagcatcgag ccagatccgc tgtaccggct gcagctgatc ggtgggccct
     2161 ttgacctcca cgaactcgta gtcccccggg gcgcggatca cgagcaggtc cgggaacccc
     2221 ttacgcagac gatgcaggtt gcgcagggtg aaatccgcca ggcgcagcag atcgccctga
     2281 ggcagctgac cgagcaccgc ttccagcaga tcctcgtcga agtgcgccca gctcaccagc
     2341 cggttggcaa cgccgaactt gcggcgccag gtgtccatga cgacgctggc cagcccgccc
     2401 tgagcggcta gcttgtccag ctgaacctcg aggatgccgg cgcgctgctc gacgaaatcc
     2461 ggccagaaga ggtccaccgg accttgctgg aacgggttga cgaaggcgcc gggaacgtcg
     2521 gcgaacacca ccggccagaa cgccaggccg gccaggccca gagagaatcg gttttccaga
     2581 tgccagcccc gcccaccgcc ctcggtcagc aggtccacgg ccagctgctc gacccggaca
     2641 ccatccccgt cgggtaggcg ctcgagccga tgagcgtggc gcttgcgccg gccgttcggc
     2701 ccgaaccgtc gggcaaagtc ctcttcctcc gccgagcgcg gctcgctgag catggcatgg
     2761 agaacgtcct gcacgccctc ggcatcgccg agacggtcga gtattcgtgc cgttcgctcc
     2821 cgagccggat gactggtgct gcgcgcatag ctggcgagcg cctggtcgta ctcgccccgt
     2881 cgttcgtgcc agcgacccag ccggttggcc gtcttgtccc gcgatcgctg agcccatcgc
     2941 gacgccggct cggtccacat cgctccgggg agccatgatg cgagcacagg ctgctcttcc
     3001 aggcgatagg aaaggctgct gagctcgaga taccgcaggt attcgtcgag ccgttcacgg
     3061 ttggcgaact gccgatgccg gggatcgagc tcgtagggct cgtaccggat gaagcccagg
     3121 tcctggagta cgaaggcgct gagatcctgg cgggtatcac cgaagaacag cagctgatgc
     3181 agccagaagg tgggaaagtc gcgaatgctc acccatggat gcacaccggc gagccgagag
     3241 agaacgtgag catcgctgta acgcgctacg cattggttga tccagcgagc cttcggcgca
     3301 tcgccaatgg cggcaaagag ctcacgtctt tccgccgtag agagcagacc gagcagcgcg
     3361 tcggccggtg cccggccgtt gcggacgacg agcccggctt cctccagctc gccaaccgcg
     3421 gccgtcacat cgctcacttc gcgatagcga agcttgtcga tacgaatcca cggcccacgt
     3481 cggctcagaa ggcgggcata cagccgctgg gcgggaaccg agcaggccac gatgcggcgc
     3541 ccgtagccga tctcctctct gcgcagcacg tcaccatagt gggcgtgcac gtgggtgatc
     3601 agctggcgca ggttctcagc gtagtagtcg ggcggcggcg ggctgttggc ggcaagctga
     3661 ggcacgatgc ttgaagccat ccggttgctt cgaacgaggc ctgtggatta taaagaggtc
     3721 tgtatacatg tacagtactc gccagctccc caatgcctgc cttttcctac tgcatctgca
     3781 ccaccgtcga cgcccgcgcg ctgcaggcgc tgctggaagc aagcggtcaa ggcagctaca
     3841 aggacgagca gccctggctc atcgctcgag agattctgga agaagccgcg tctcagtcag
     3901 cccgcctgcc catccttttc gccagcgggc atccttccga gttctcgcac tggggattca
     3961 tcgaatccat cgacgtggtc gagctgcacc gcggaacctg ggaatcacga tgcgcgttca
     4021 gcccgctgca acccgtaaac cccatctgga cgagcataga cagcgtcttc ctgaagccca
     4081 cgggcgagca gctcagacga gaggcgctgg aaggaattgc gcaccatcga tatccgctca
     4141 acgaaggcga gctgcatccg tacgcgatct gcgagacgcc agcgttcata ctggcttaaa
     4201 ggagagtgac cgggttgagc tgaggccgct caggcgacgc cgcggctggg atctgacgag
     4261 tcttctttgt cttcgcggcc tgccgctttc ggctcgccgt tgatgcgcag ccagacttcc
     4321 tttcggtgca cggcagaacg tttcggcgcg tcgattccca gatgcacctg gctaccgcga
     4381 atcttgagca ctttgagctc tatatcgtcg tcgatcatga tcgactcccc agcacgacgg
     4441 gtgaggacca gcatccaggt ttccttgcaa tccttttctc cgtagaaatc tactgacatt
     4501 gactgattcg ttctgtgacg tgcacgcggc tttggccggg cagcggcgag aaaggagaac
     4561 tggttggcta atcgtctgag cggtcggtcg cttcatccag aagggacttg ggcagcggcc
     4621 tcttgacctg ggcgcccagc tcacggaagc gctccacctg cctgaccagg ttgcccttgc
     4681 cgctggcgag ctttccgtag gctgcctggt aggttttctc gacggtgcca agctgcttgc
     4741 cgagcagatc catgtcctcg atcagtcctc tgagcttgtc gtaaagagcc cccgcccgcc
     4801 gcgcgatgtc ctgggcattc cggttctgct tttcgaaccg ccagacgttg tgaatgatcc
     4861 gaagcgtcat catcagcgtc gtggggctca cgatgacgat gcgcttctcg aacgcctccg
     4921 tgaacaggcg atgatcgtgc tccatggcca tggtgaaggc cgcctcgatg ggcacgaaga
     4981 gaagaacgaa atccagcgag cggacgtcgc tgagctgatc atagtcctgc tcacccagac
     5041 gcttcacatg agcgcgaagg ctgaccaggt gctgacgcag ggcaagctcc cgggcgccgt
     5101 ccgtctcggc gctcaaggct tcctcgtacg ccaggagaga gaccttggcg tccaccacca
     5161 cgtctttgcc gtcgggcagg cggatcagca catcgggcct tttcagctct ccccgttccg
     5221 agcggcgggc agcctgcaga aagtactcgt gcccttccct cagccccgac tcctccagca
     5281 cgcgctccag cacgacctcg ccccagtttc cctggacctt gacgtcacct ttgagggcct
     5341 tggtgaggtt ctcggcttct tcgttgatcc gctcgctggc cttctggagg ttgcgcactt
     5401 cattgagcag cgaggcacga tctttggtgt cggtgtgata gacctgctct acccgcttgc
     5461 ggaaatcgac gatctgctcc ttgaaggggg tgagaacgct ggacagccgc tcctggtgtt
     5521 tgaccccctg ctgatcgaag atcttcgtgg cgagcagctc gaactcctgc ttgagcgctt
     5581 cgctggattc cttgaagacg gcttctttct cggcgaaccc ctttcgcgac tcctcgagtc
     5641 gagcctcgac ggccgcctgg gccgcctggc gatcactgag ctccttcgtt cgctcgtcca
     5701 gcgctgagcg tgccgagttc agctggttct cgaacgaagt gatccgctcc tccagccgga
     5761 ccagatcccg ctcacgctcg cccagccgct cttcgagcac cagacgttcg gcctggcccg
     5821 cctcggcctg ctgaagctgc ccggcggcat acgaggctcg cgcctgcacc cggcagcgcc
     5881 acagccagac gacgcttgcg agcgcggcgc cccccgcgaa gagcccgacg ccctggaccc
     5941 agagatctat gccgtgcctc ccgcctcccc ctgggcggct ctggccccga tgaagtcggg
     6001 cgtccgctta tccagaaaag cggcaacggc ttccttgtgc tccgggctgg cgaacgcctg
     6061 ctgcaaaagc ttgccctcca gccgctggac ctcgtcgaga tcggtggcac acccgttttc
     6121 ggtcagcagc tgcttcaccc agcgcagctg cgggccggga ttcgccgcaa tggcctgagc
     6181 gagatccatg gccttgtcca tcagcgcatc gtcggcgacc agatgatcca ccagacccat
     6241 cgcatgcgcc tcgctcgccg gatacatttt tccggtgagg cacatctcgc tggcacggcc
     6301 gaaccccatg cgctggacca ggaagtggga gctcgcaagc tcgggaacca gacccatctt
     6361 gatgaacccc atggcaaact tggcggaatc ggccgccacg atgatgtccg ccggcaggat
     6421 catggtggcg cccacgccca cgctggcccc gttgaccgcc gcgatgatgg gcttggactg
     6481 gcgcagcagg cgtacccagg gcgtcgcgtc gtcaccgcga tcgtcgttgc cgtcgaggcg
     6541 tgctttgaaa ttgtgctcga tgtcggcacc tgcgcagaag ccgcggcccg cgccggtgat
     6601 gatgatggcg ccgatggcgg ggtcctcgtt gcacacgccg atggcgtgcg agagctcccg
     6661 gctcatctgg taggtccacg cgttcattcg ctccggccgg ttcagcgtga gaacggcgat
     6721 ggaatcctgc gcctcgaata ggatctgctc gtagccggtc accttggtct cctctgcctc
     6781 attcgggcct aagtatgagg caatcgggcc gtgaagccac ccggggcgat cggctggtct
     6841 ggcgagacag gactcagtag cccgtcttct cccgctcctg ctccgcggtg ccccgcgcgg
     6901 ccgcaagctc ctgggcggcg gtgcgcgtca tgaaaccggc gtcgctgccc agggtgacga
     6961 agtcgaaccc ggcagccagg cgtttctggc agtagtccag gcttgaggtg tgaatgcccg
     7021 ccggcagctc atggcgatga cagacctcca gaatgcgggt gacttccgcc acgtgctcgg
     7081 gcacgtccgt gtcgccaagc gccggaaggc ccagcgcaag cgccagatcg gccggtccga
     7141 tgtagacccc gtccacgccc ggcgtggtca cgatctcctc gagcttgtcc aggccctcgc
     7201 gggtctccac catcacgatg caggccatct gctcgttggc ctgggccgca tagccacgcc
     7261 cgccgtaaag cgccccgcga atgggcccga aagagcggct gcccagcggc gggtagcgac
     7321 aggcgtcgac ggcccgcgcc gcgtcttcgg cgttgttgac catgggcacg atgacgccgt
     7381 aagcgccggc gtccagcgcc ttcatgatct cgtagggctc gttccagggt acgcgcacca
     7441 gcggcgtcga tgtgcctcgg gacacggcgg gcagcatgta ggtcagatcc tggtagtcga
     7501 ccaggccgtg ctgcagatcg atgcacacgt agtcgaagcc gagctgtccc agcgcctctg
     7561 cggagtacgc gtttcccatg gagagccagc agccaatggt ctgccggcct tcccgccacg
     7621 ctgccagcaa tgcgttctcg cgcacgttct tccctccccg gatctacccc caaaccttat
     7681 cacaggtaag gaatgctgct gacactcgaa acgccggcgt gcgaaaatcg gcgttctcat
     7741 ttgcacccgg cggagc
//
LOCUS       DBNHKV010000087         6794 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_297403, whole genome shotgun sequence.
ACCESSION   DBNHKV010000087 DBNHKV010000000
VERSION     DBNHKV010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_297403"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..902
                     /locus_tag="AC16PP_02795"
     CDS             102..902
                     /locus_tag="AC16PP_02795"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6480581.1"
                     /translation="MQGRVALVTGGARGIGRGIAQAFLQAGARVMIGDLGGAGADWKY
                     ELSAESELKRTADELSAMGETHCVAVDVTDAASCARAVDATVQAFGRLDVLVNNAGVV
                     ASGDTESFPEADWDRVFAVNAKGIFLMTRAALPALRRSADAAIINTASIAGKRGFPKM
                     AAYCGSKFAAIGITQSLAAELAPEGIRVNALCPGMVGTAMWLEHLLPANAAKPDEKDD
                     QFKEAMARTIPMGRPQTPQDMGHAAVYLATAANVTGVSLSVAGGYEMN"
     gene            899..1708
                     /locus_tag="AC16PP_02800"
     CDS             899..1708
                     /locus_tag="AC16PP_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR6480582.1"
                     /translation="MKKPTAVQNLFREPLLYFLLAGAAIFALAELTDGPGDDYLIEVS
                     DAERARISDQWQAQMSRAPTDEELAGLIEQWLREEIYYREALRMGLDRNDIIIRRRLA
                     QKLTFLTEDVIAGSTPSTEELRSYFEANAERYRIPERITFRHRYFSAERRPNAREDAA
                     AALTDEAVRGDPFMLQKAYALRSSREIADLFGAEFAEGLAQVAPGSWQGPIRSAYGWH
                     VVLVEQRAPGRLPSLEEVQKRAATDLSQERRSRANDEYYHSLRQRYEIIGG"
     gene            1705..2670
                     /locus_tag="AC16PP_02805"
     CDS             1705..2670
                     /locus_tag="AC16PP_02805"
                     /inference="COORDINATES: protein motif:HMM:NF025167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HIR6480583.1"
                     /translation="MKALAALLVVLAASQAKAHEVRPAYLQLTETAPGQFEVLWKQPL
                     LGDRRLPLEPVLPEACAREGPPIPEVAGAALIERWRTDCDLRSGAVTISGLSKTLTDV
                     LVEIRYLSAEPVSQLLKPSRPSLDLASPTPQIWSYLTFGVEHLLLGLDHILFVVGLVL
                     FIGNTWTLVKTITGFTIAHSITLACSVLGLISLPQAPVEAVIALSILFLARELVEPPE
                     RRSRITLGRPWLMAFAFGLLHGFGFAGALTEIGLPADQLAVSLLLFNLGIEIGQLLVI
                     GLLLGAAAIFRKAWRRNREVWPRRFAWVMGSVAGFWTIDRVLLVF"
     gene            complement(2703..3110)
                     /locus_tag="AC16PP_02810"
     CDS             complement(2703..3110)
                     /locus_tag="AC16PP_02810"
                     /inference="COORDINATES: protein motif:HMM:NF016060.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR6480584.1"
                     /translation="MTDFRTQSRRQVFRYLQVGFAGFVTDVAVLSALVYGMSLGETET
                     GLVGSRFVSFLAAVTVTFLLNARYTFGASIRDSRFPVYVLVQSLGAAITLGTYTALVL
                     HTTLSQAPVVALIIGSAAATTSNYVLVRRFVYV"
     gene            3234..3719
                     /locus_tag="AC16PP_02815"
     CDS             3234..3719
                     /locus_tag="AC16PP_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR6480585.1"
                     /translation="MTTAPIECPAQTVEDGWIDYNGHMNMAFYNLAFDRALDHVYDRL
                     GIGADYVRLRGGSCFTREVHVNYLRELSAGDPIRVTFQLLDWDAKRLHFFEEMYHADQ
                     GYLAATSEQLALHVSMTSRSTHPFPQDIQAHLEELMTAHRPLPTPAQVGRVMGIRRSP
                     R"
     gene            complement(3735..5408)
                     /locus_tag="AC16PP_02820"
     CDS             complement(3735..5408)
                     /locus_tag="AC16PP_02820"
                     /inference="COORDINATES: protein motif:HMM:NF027688.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcineurin-like phosphoesterase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6480586.1"
                     /translation="MRLCCCVALALLGLGWSSFGLGSGTANGKVFEDLDGDGVWEPGE
                     PGVKGVRVSNGIAVVLTGEDGGYTLPVPEEAVIFITKPSGYATPVNAHQLPQFYYVHQ
                     PDGSPDHLRYPGVDPTGPLPERIDFPLRRQDEPARFQAILFADTQPQTEEELDFIRDD
                     VIAELIGTGARFGMTMGDILFDELSLFPRYNALIGTLGIPWYNVPGNHEMNFLAADDR
                     HALETFKRYFGPPYYAFEYADALFVVLDNIEYLGNGESDPADVRGTGGYVARIGRRQL
                     DWLAAELRHVPVDKLVFLGMHAPLATYLDDSPRNNTADRRELFGLLKGRPNLYAVAGH
                     THTTEHHYFGRDEGFAGPGELHHHVLSTVSGAWWSGPFDTRGVPTSDQRDGTPNGYHV
                     LEVDGTKVTVRYKAASAPADYQMRIMFDVVHHGLRKDAMRDFRHGQLFEGRFSVDQVP
                     AADILVNLFDGGPRSEVAFRIGDGEYLPMRRVRRADPYLIELHARNAETKKSWFKAEP
                     SSHIFVADVPDDLGPGVYPVGVRAIDEFGRAHHAHAVLEITGGSSPSGL"
     gene            complement(5421..6566)
                     /locus_tag="AC16PP_02825"
     CDS             complement(5421..6566)
                     /locus_tag="AC16PP_02825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6480587.1"
                     /translation="MPMILNDEQNMLKDSAKDFCSNSAPISQLRRLRDENNADGFDRG
                     TWQAMVDLGWAGIPWPEEHGGLDFGYKGLGVVTEETGRTLAASPLFASVWVCGTLVNL
                     GGSEEQKGELLPLISTGELLMSLALEESHRHDPYGLETRAAAADGGFVLNGRKTFVLD
                     GHVADKLVVAARTSGERGDRNGITLFVVDSDAEGLSVTRTVMVDSRNAANVELDGVRV
                     GADAVLGAVGGAAEVLDPALDIARIGLCAEMLGSLQECFERTVEYLKEREQFGVPIGS
                     FQALKHRAANMFCEVELAKSCVLEALTALDENRDPQEIARLASLAKAKVGETFHLVSR
                     EGIQMHGGIGMTDEFDIGFFIKRAAVTEQTFGDVGFHRNRYGELEGY"
BASE COUNT         1215 a         2283 c         2170 g         1126 t
ORIGIN      
        1 cggctgctcg tcatcgacgc tcagttcggc aacgccgact accatttgca gcgcttcgcg
       61 gcctaagtag aatccggcct ccgcacaggg ggaagcacgt catgcagggc agagtcgcgc
      121 tggtaaccgg cggcgcccgc ggcatcggcc ggggcatcgc acaggccttt ctgcaggccg
      181 gggctcgggt gatgatcggc gacctcggcg gcgccggggc cgactggaag tacgagctgt
      241 ctgccgaatc ggagctaaaa cgcacggcag acgagctgtc ggccatgggc gagacccact
      301 gtgtggccgt cgacgtgacc gacgccgcca gctgcgcgcg cgcggtggac gccaccgtgc
      361 aagccttcgg aaggctcgac gtcctcgtca acaatgccgg cgtcgtagcc tcgggagaca
      421 cggaaagctt ccctgaagcc gactgggatc gcgtgttcgc ggtgaacgcc aaggggatct
      481 ttctcatgac ccgggccgcc ctgccggcgc tgcggcgatc ggccgacgcg gccatcatca
      541 atacggcttc catcgcggga aagcggggct ttccgaagat ggccgcttac tgcggctcca
      601 agttcgccgc cataggaatc acccagtccc tggcggccga gctcgcgccc gagggcatcc
      661 gcgtcaacgc cctgtgtccc gggatggtgg ggaccgccat gtggctcgag cacctgcttc
      721 ctgccaacgc ggcgaagccg gacgaaaaag acgaccagtt caaggaggca atggcgcgca
      781 ccattcccat gggccggccc cagacccctc aagacatggg ccacgcagcc gtctacctag
      841 ccacggccgc caacgtcacc ggcgtcagcc tgagcgttgc cggcggctac gagatgaatt
      901 gaaaaagccg acggccgtgc agaacctttt ccgcgaaccg ctgctttact tcctgctggc
      961 aggcgcggcc atcttcgccc tcgccgagct cacagacggc cccggcgacg actacctgat
     1021 cgaggtttcc gacgcggaga gggcacggat ctcggatcag tggcaggccc agatgagccg
     1081 ggcgccgacc gacgaggagc ttgcgggcct gatagagcag tggttgcgcg aagagatcta
     1141 ctatcgggaa gcccttcgaa tggggctgga tcgcaacgac atcatcatcc ggcgccgtct
     1201 tgcccagaag ctcacgttcc tgaccgagga cgtcattgcc ggttccaccc cctccaccga
     1261 agagcttcga agctacttcg aagccaacgc ggagcggtac cgcatacctg agcgaatcac
     1321 tttccggcac cgctacttca gcgccgagcg ccggccgaat gccagagagg atgccgccgc
     1381 cgccctgacg gacgaggccg tacgcgggga tcccttcatg ctccagaaag cctacgcgct
     1441 gcgctcgagc cgggagatcg ccgatctttt cggcgccgag ttcgccgaag ggctggccca
     1501 ggtggcgccc gggagctggc agggacccat ccgctcggcc tacggatggc acgtggtgct
     1561 cgtggagcag cgggctcccg ggcgactgcc gtcgctcgag gaagtgcaga agcgggcggc
     1621 gacggacctg tcacaggaac gccgaagccg agccaacgac gagtactacc actcgctgcg
     1681 tcagcggtac gagatcatcg gcggatgaag gcgctggcgg cgctgctcgt cgttctcgct
     1741 gcgagccagg ctaaggccca cgaggtccgc ccggcctacc tgcagctgac ggaaaccgca
     1801 ccgggccagt tcgaagtgct ctggaagcag ccgttgctcg gcgaccggcg cctgcccctg
     1861 gaaccggtgc tgcccgaggc gtgcgcgcgc gagggcccgc cgatcccgga ggtcgccggc
     1921 gctgcgctca tcgaacgctg gcgcaccgac tgcgaccttc gaagcggagc cgtcaccatc
     1981 tccggcctct ccaagacgct gaccgacgtt ctcgtggaga ttcgctacct gtcggcagag
     2041 ccggtttccc aattgctcaa gcccagcagg ccatccttgg atctggcgtc accgaccccg
     2101 cagatctgga gctacctcac gttcggcgtg gagcacctgc tgctcgggct cgaccacatc
     2161 ctgttcgtgg tgggcctcgt gctattcatc ggcaacacct ggactctggt gaaaaccatc
     2221 actggcttca ccatcgccca cagcatcacg ctggcctgct cggtgctggg gctgatctcg
     2281 ttgcctcagg cccccgtgga agcggtgatc gcgctgtcga tcctctttct tgcccgggaa
     2341 ctggtggaac ctcccgagcg gcgctcgcgc atcaccctgg gccgcccgtg gctgatggcc
     2401 ttcgctttcg gtctgcttca cggcttcggg tttgccggcg cgctcaccga gatcgggctg
     2461 cccgcagatc agctggccgt ctctctgctg ctgttcaacc tcggcatcga gatcggccaa
     2521 ctcctcgtta tcggcctgct cctgggcgct gcggcgattt tcaggaaggc ctggaggcgc
     2581 aaccgcgagg tctggcccag gcggttcgcc tgggtcatgg ggtccgtcgc ggggttctgg
     2641 accatcgacc gggtgctgct cgtcttctga gatcgatctg gctgccgccg cgaacccgat
     2701 gctcacacgt agacgaagcg tcgcaccagc acgtagttgc tggtggtcgc tgccgccgaa
     2761 ccgatgatca gggcgaccac gggcgcctgg gacaaggtgg tgtggagcac cagcgcggtg
     2821 taggtgccga gggtgatggc agcccccagc gactggacca gcacgtagac ggggaacctc
     2881 gagtcccgga tcgaggcgcc gaaggtgtac ctggcgttga gaagaaaggt caccgtcacc
     2941 gcagcgagga aagaaacaaa gcggcttccc acgagccccg tctcggtctc accgagagac
     3001 attccgtaga ccagcgccga gagcacggcc acgtcggtga cgaagcccgc aaagccgacc
     3061 tgaaggtagc ggaacacctg tcggcgggac tgcgtgcgga aatctgtcaa ttcacacctc
     3121 gtccaggcac cttcacccca agcttacgtg gcagccatgt gcccagcaag gcgaccgcct
     3181 tagcctgccg gcgctttggg gttaccatcg ggaaagactc tggggagacg ctcatgacca
     3241 ccgcacccat cgaatgcccg gcacaaaccg tggaggatgg ctggatcgac tacaacggcc
     3301 acatgaacat ggccttctac aacctcgcct tcgaccgggc cttggatcac gtctacgacc
     3361 ggctgggcat cggcgcagac tacgtacggc tgcgcggcgg ctcctgcttc accagggaag
     3421 tccacgtgaa ctacctgcgc gagctaagcg ccggcgaccc gatccgggtg acgtttcagc
     3481 tgctggactg ggacgctaag cggctgcact ttttcgaaga gatgtaccac gcagaccagg
     3541 gctaccttgc cgctacctcg gagcagctgg cgcttcacgt gagcatgaca agccgatcaa
     3601 cgcacccctt tccccaggac atccaggcgc atctcgagga actcatgacg gcacatcggc
     3661 cgcttccgac gccggctcag gtcggacggg tcatgggcat cagacgcagc ccccgctgaa
     3721 acggcgcagg aaaatcagag gcccgacggc gagctgccgc cggtgatttc cagcaccgca
     3781 tgcgcgtggt gcgcgcgacc gaactcgtcg atcgcacgca cgcccaccgg gtagacgccc
     3841 ggaccgagat cgtcgggaac gtccgcaacg aagatatgcg acgacggctc ggccttgaac
     3901 caggacttct tcgtctcggc gttgcgcgcg tgcagctcga tcagataggg atccgcgcgc
     3961 ctcacccggc gcatcggcag gtactccccg tcaccgatgc ggaatgccac ctcggagcgc
     4021 ggacctccat cgaagaggtt gaccaggatg tctgctgcgg ggacctgatc cacggagaaa
     4081 cgaccctcga acagctgccc gtggcggaag tcacgcatgg cgtccttgcg cagaccgtgg
     4141 tgcaccacgt cgaacatgat gcgcatctgg tagtcggcgg gcgcgctggc agccttgtag
     4201 cgcacggtca ccttcgtgcc gtccacctcc aggacatggt acccgttggg ggttccgtcg
     4261 cgctgatccg acgtgggcac gccgcgggta tcgaacggcc cgctccacca cgccccggaa
     4321 accgtgctga gcacgtgatg gtggagctca ccgggcccgg caaatccttc gtccctgccg
     4381 aagtagtgat gctcggtggt gtgcgtgtgg ccggcgacgg cgtacagatt gggccggcct
     4441 ttgagcaatc cgaagagctc acgccggtcg gcggtattgt ttctcgggct gtcatcgagg
     4501 taggtggcaa gcggggcgtg catgccgagg aaaaccagct tgtcgacggg cacgtggcgc
     4561 agctctgccg ccagccagtc gagctgccgc ctgccgatcc tggcaacgta gccgccggtt
     4621 ccccggacgt cggccggatc gctttcgccg ttaccgagat actcgatgtt gtccagaacc
     4681 acgaagagcg catccgcata ctcaaaggcg tagtaaggcg gaccgaaata ccgcttgaac
     4741 gtctcaagcg cgtggcgatc gtcagccgcc aggaaattca tctcgtgatt gcccggcacg
     4801 ttgtaccagg gtatgcccag ggtcccgatg agcgcgttgt agcgcggaaa aagcgagagc
     4861 tcgtcgaaca ggatgtcacc catggtcata ccgaagcgcg cgccggtgcc gatgagctcc
     4921 gcgatcacgt cgtcccggat gaaatccagc tcctcctcgg tctggggctg ggtgtcggcg
     4981 aacaggatgg cctggaaacg ggcgggttca tcctgccggc gcagcgggaa atcgatacgc
     5041 tcgggcaacg ggccggtggg atccaccccg gggtagcgca gatggtcggg cgagccgtcc
     5101 ggctggtgga cgtagtagaa ctgcggcagc tggtgagcgt tcaccggcgt tgcgtaaccg
     5161 ctgggcttgg tgatgaagat cacggcttcc tcgggaaccg gcagcgtgta gccgccgtcc
     5221 tcgccggtca gcaccacggc gatgccgttg gagaccctca cacccttcac accaggctcg
     5281 ccgggttccc agacgccgtc gccgtcaaga tcctcgaaga cttttccgtt cgccgttccg
     5341 gagccgagcc cgaagctcga ccagcccaga ccgagcaggg ccagcgcgac gcagcagcac
     5401 agcctcaacg tcccgggccc ctagtaacct tccagctctc cgtatcggtt ccggtggaag
     5461 cccacgtcac cgaaagtctg ctcggtcacc gccgctcgct tgatgaaaaa cccgatgtcg
     5521 aactcgtcgg tcatcccgat gccgccatgc atctgtatgc cttcccggga aaccaggtga
     5581 aacgtctcgc ccactttcgc tttcgcgagg ctagccagcc gggcgatttc ctggggatcg
     5641 cggttctcgt caagcgccgt cagggcttcg agcacacagg atttggcaag ctcgacctcg
     5701 cagaacatgt ttgccgcgcg gtgcttgagg gcctgaaagg aaccgatggg cacgccgaac
     5761 tgctcgcgct ccttgagata ctcgaccgtg cgctcgaagc actcctggag gctgcccagc
     5821 atctcggcgc agaggccgat gcgggcaatg tcgagcgccg ggtccagaac ctcggccgcg
     5881 ccgccgacgg cgccgagcac cgcatcggcg ccgacccgaa cgccgtccag ctcgacgttc
     5941 gcagcgttgc gcgagtccac catgacggtg cgtgtcaccg acagcccttc ggcgtcggaa
     6001 tccacgacaa acagcgtgat cccgttccga tccccccgct cacccgaagt ccgggcggcg
     6061 accacgagct tgtcggccac gtgcccgtcg agcacgaagg tcttgcgccc gttcagtacg
     6121 aacccgccgt ctgcggcggc ggccctggtt tcgagcccgt aagggtcatg acggtgagac
     6181 tcctcgagcg cgaggctcat cagcagctca ccggtggaga tcagcggcag cagctcgccc
     6241 ttctgctcct cgctgccacc cagattcacg agggtgccac acacccagac gctggcaaaa
     6301 agcgggctgg ccgccagggt ccgaccggtt tcttcggtca ccacgcccag gcccttgtac
     6361 ccgaagtcga gccccccgtg ctcctccggc cagggaatgc ccgcccagcc gagatccacc
     6421 atggcctgcc aggtgccccg gtcgaagccg tctgcgttgt tctcatcgcg cagcctgcgc
     6481 agctgcgaaa tgggcgcgct gttgctgcag aagtccttcg cgctgtcctt gagcatgttc
     6541 tgctcatcgt tgaggatcat cggcatgatc gcccccggaa tcatcgagcg gccaatacaa
     6601 ccgcgtcgca ccgacgctgt caatccgaag gctgctgacg ggtgtaaaac ccctcgaacc
     6661 agggctgcca gacgccggtt tcgcgcgact cctcaaagta ctgccgcacc ctgccgtcgg
     6721 gtagccgggt ccagctgccg cggaagggga agcgttcccg ggaggcgcgg taggtgatgg
     6781 tgcccgacag aacg
//
LOCUS       DBNHKV010000088         4122 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_85228, whole genome shotgun sequence.
ACCESSION   DBNHKV010000088 DBNHKV010000000
VERSION     DBNHKV010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_85228"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="AC16PP_02830"
     CDS             complement(<1..478)
                     /locus_tag="AC16PP_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007946297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HIR6480588.1"
                     /translation="MPGLLPNVDPDGLLEYSVVYTDRALNHMSLAFRQVMRDISESLK
                     SVYGAASSVVVPGSGTFGMEAVARQFATGRKCLVLRNGWFSYRWSQILEMGRMPTETI
                     VLKARQAEPGTQAPFAPAPLDEVVASIAAEKPNVVFAPHVETSCGMILPDDYLREVA"
     gene            complement(505..597)
                     /locus_tag="AC16PP_02835"
     tRNA            complement(505..597)
                     /locus_tag="AC16PP_02835"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(561..563),aa:Sec,seq:tca)
     gene            complement(610..2481)
                     /gene="selB"
                     /locus_tag="AC16PP_02840"
     CDS             complement(610..2481)
                     /gene="selB"
                     /locus_tag="AC16PP_02840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HIR6480589.1"
                     /translation="MIVTLAGHVDHGKTSLVRCLTGQDTDRLEEEKRRGLTIDLGFAY
                     VSGDHEVDPIGFVDVPGHHRFIHNMVAGVASMQYALMVVAADDGPMPQSREHLQILEL
                     LGISQGAIALTKCDRVDQARLREAEDEIRDLTADSFLADAPIFRTSATSNEGISELLN
                     HLQKVNGEFRVDQPPRQFRLAVDRAFTVRGSGLIATGTVHAGSIVNDDELAIFPAAVR
                     ARVRGIRTQSREAKEARAGDRCALNLAGVSLDQVGRGSWLSASSFEGSRDLTLELRVV
                     ADFPRPVKHWTPVHVYHATSHATGRIALLDRDVLAPEESCLADLVLDEPLLAIHGDRV
                     VLRDQSLDLTLGGGRVVHNARPRGRRRAPARLEFLDAFAKQTPEACFEQAVRHAPVPL
                     EEFQRSWDLLPGHLDRVVEASQSRVHDGFAVAEGLWREWADGIVQEVERRHEEQSGLQ
                     GLKANQVGAPVPERFKSELLRELAGAGKLTQRAGHYLPAEHEVALSESEARLLDRISG
                     QLDQIQPPSVGDLAKQIGVPVAELKRQLQPLAGKRRLVRVSETRYYLPAPLSSLVHVV
                     DALSSKGPFTVREFRDAADIGRNVAIEVLEYLDGRGYTKRQGDVRRVVGDPSRVMAT"
     gene            complement(2478..3845)
                     /gene="selA"
                     /locus_tag="AC16PP_02845"
     CDS             complement(2478..3845)
                     /gene="selA"
                     /locus_tag="AC16PP_02845"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HIR6480590.1"
                     /translation="MPRLRDLPSIDRLLRSPKLAFLVHAHGAETIKQSLRSIQAAWRA
                     TGEVPEWATDPEEYARRLEDQLAGASYRPVFNLTGTMIHTNLGRALLDPEMFRSIEPL
                     VTRPVNLEFDLASGRRGDRDRVVESRLQVLTGAPAATVVNNNAAALMLCLNTAALGRE
                     VIVSRGELIEIGGSFRLPELMERAGCRLVEVGTTNRTHLRDYQAAIGERTGMLLKVHP
                     SNYHISGFTHDVGVAELAALAREHRLPLCVDLGSGTLVDLRKWGLPHEPMPQEILAQG
                     ADLVTFSGDKLLGSVQAGIIVGDSENVGAIKRNPMKRALRPDKLTLAFLDRTLRLYEN
                     PERLAERLPLLRSLTRTLGELENQGEVLARSLETVLGDFDVRLVPSEVQIGSGSLPNQ
                     RIASRAVRITHDDPSEVRRLGERLRRLATPVIGRLHQEAVWLDVRGAEPLDELIEVLH
                     GLAAP"
     gene            complement(3899..>4122)
                     /locus_tag="AC16PP_02850"
     CDS             complement(3899..>4122)
                     /locus_tag="AC16PP_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR6480591.1"
                     /translation="ERKLYPNVDFYSGIILKAIGIPTEMFTVIFATGRTPGWIAHWNE
                     MIGAPYKIGRPRQLYQGATPRDYVPLAER"
BASE COUNT          682 a         1319 c         1399 g          722 t
ORIGIN      
        1 tggcaacctc gcggagatag tcgtcgggca ggatcatgcc gcacgacgtc tccacatggg
       61 gtgcgaaaac gacgttgggc ttctcggcgg cgatgctggc aaccacctcg tcgaggggcg
      121 ccggggcgaa aggcgcctgg gtgcccggct cggcctgccg ggccttcagc acgatggttt
      181 ccgtgggcat gcggcccatc tctaggatct ggctccagcg atagctgaac catccgttac
      241 gcaggacgag acattttctg ccggtggcga attgcctggc gacggcctcc atgccgaagg
      301 tcccgctgcc gggtaccacc acggagctcg ccgcgccgta gaccgacttg agtgattcgg
      361 agatgtcccg catgacctgc cggaacgcaa gcgacatatg attgagtgcc cggtcggtgt
      421 acaccaccga atactcgagc aggccgtcgg ggtcgacgtt gggcagcagt ccgggcatgt
      481 gttcctcgtc agtcagcagg aaaatggcgg aagaggtagg agtcgaaccc accaggcacg
      541 tcatcgcacc tcaacggctt tgaaggccgc gcgccccacc ggggacgatg ctcttccgaa
      601 ggcagtttat catgtcgcca tcactcggct cgggtcaccg accaccctcc ggacgtcccc
      661 ctggcgcttt gtgtagccgc ggccgtccag atactcgagc acctcgatgg ccacgttgcg
      721 cccgatgtcg gcagcgtccc gaaactccct gacggtaaaa ggccctttgc tgctcaaggc
      781 gtcgacgacg tggaccaggg acgacaacgg ggcgggtagg tagtagcgcg tctcgctcac
      841 gcgcaccagg cgccgcttgc cggcgagagg ctgcagctgg cgcttcagct cagccaccgg
      901 cacgccgatc tgcttggcga gatcgccgac gctgggaggt tggatctgat cgagctgacc
      961 ggaaatgcga tcgagcagtc gtgcttctga ttcgctcagc gcgacctcgt gttccgcggg
     1021 caggtagtgc ccggcccgct gagtgagctt tccagccccg gccagctcgc gcagcagctc
     1081 ggacttgaac cgctcgggta ccggcgcgcc gacctggttg gcttttagtc cctggagccc
     1141 ggactgctcc tcatgtcggc gctcaacttc ctggacgatg ccgtcggccc actctcgcca
     1201 caggccctcg gccacggcga agccgtcgtg cacgcgggat tgtgaagcct ccacgacgcg
     1261 atccagatgg ccgggcagaa gatcccagct gcgctgaaac tcctcgagcg gaacgggcgc
     1321 gtggcgtacg gcctgctcga agcaggcctc gggcgtctgt tttgcgaacg cgtccaggaa
     1381 ttcaagccgt gcgggcgcgc gccgacgtcc tcgaggacgg gcattgtgca ctacccggcc
     1441 acctcccagg gtcagatcca ggctctggtc gcgcagcacg acgcgatcgc cgtggatcgc
     1501 gagcaggggc tcgtcgagca ccaggtcggc caggcagctc tcctctggcg ccaggacatc
     1561 ccgatcgagg agggcaattc tgcccgtggc gtgtgaggtg gcatgataga cgtgcacggg
     1621 agtccagtgc ttgaccgggc gcggaaagtc ggcgacgacc cgcagctcga gcgtgaggtc
     1681 gcggctccct tcgaagctgc ttgctgagag ccagctaccg cgacccacct ggtccaggga
     1741 gacgcctgcc aggttcagcg cgcagcggtc cccggcccgg gcctctttgg cttcgcggct
     1801 ctgggtacgg atccctctca cccgagcgcg cacggcggca ggaaagatcg ccagctcatc
     1861 gtcgttgacg atggatccgg cgtgaacggt gccagttgcg atcaggccgg aacccctgac
     1921 ggtgaacgcg cggtcgacgg cgagccggaa ttggcgcggg ggctgatcca cgcggaactc
     1981 gccgttgacc ttctggaggt ggttgagaag ctcggagatg ccctcgttgg acgtggctga
     2041 cgttcgaaat atgggggcgt ctgccaggaa ggagtcagcg gtcagatcgc gaatctcatc
     2101 ttcggcttcg cggagcctgg cctgatcgac ccgatcgcat ttggtcaggg caatggctcc
     2161 ctgggagatt cccagcagct ccagaatctg caggtgctcg cggctctggg gcatgggccc
     2221 gtcgtcggcc gcgacgacca tcagggcgta ctgcatggac gccacccctg ccaccatgtt
     2281 gtggatgaac cggtggtggc cgggcacgtc gacgaagccg atgggatcca cctcgtggtc
     2341 gccgctgacg taggcaaagc ccaggtcgat cgtgagcccg cggcgctttt cttcttcgag
     2401 ccggtccgtg tcctgccccg tcaagcagcg gaccaggctg gttttgccgt ggtcgacgtg
     2461 cccggcaagg gtaacgatca cggcgcggcg agcccgtgca gcacctcgat gagctcgtcg
     2521 agcggctcgg cgcctcgaac gtccagccag acggcctcct gatgaagcct gccgatcacc
     2581 ggcgtggcta gcctacgcag acgttcgccg agcctgcgga cctcggaggg gtcgtcatgg
     2641 gtgatgcgca ctgcccggct cgcgatgcgt tggttgggca ggcttccgct accgatctgc
     2701 acttcgctgg gcaccaggcg cacgtcgaag tcgcccagaa cggtctccag tgatcgagcc
     2761 agcacttcgc cctggttttc cagctcgccc agggtgcggg tgagcgagcg caggagggga
     2821 agcctttcgg ccagacgctc ggggttttcg tagagcctga gagtccggtc cagaaaggcc
     2881 agggtcaact tgtccggacg cagagcgcgc ttcatggggt ttcgctttat cgcgccgacg
     2941 ttttccgagt cgcccacgat gatgcccgcc tgaacggagc ccagcagctt gtctccgctg
     3001 aacgtcacca gatctgcgcc ctgggcgaga atctcttgag gcatgggttc gtgaggcagc
     3061 ccccacttcc ggaggtcgac gagcgtgccg ctgccgaggt ccacacacag gggcagccgg
     3121 tgctcccgtg ccagagccgc cagctccgcg acgccgacat cgtgggtgaa accgctgatg
     3181 tgatagttgg atggatgcac tttcagcagc attcccgttc gctcgccgat cgcagcctgg
     3241 tagtcgcgaa ggtgggtgcg gttggtggtt cccacctcca ccaggcgaca cccggcgcgc
     3301 tccatcagct cgggaagcct gaagctgccg ccgatctcga tgagctcgcc acgggaaacg
     3361 atcacctccc gcccgagcgc ggccgtgttc aggcacaaca tcagggcggc cgcgttgttg
     3421 ttgaccacgg tggcggccgg agcaccagtg agcacctgca gccgggactc gaccacccga
     3481 tcccggtcgc ctcgccggcc gctggcaaga tcgaactcca ggttcacggg ccgggtcacc
     3541 agaggctcga tggaccggaa catctccgga tcgagcagag cgcggcctag gttggtgtgg
     3601 atcatcgtcc cggtgaggtt gaataccggg cgataggagg cgccggcgag ctgatcttcc
     3661 agccgccgcg cgtactcctc gggatccgtt gcccactccg ggacctcgcc ggtggcccgc
     3721 caggccgcct gtattgatct caatgactgc ttgatggttt ccgcgccgtg ggcgtggacc
     3781 aggaaggcga gcttcgggga gcgaagcagc cggtcgatgc tgggcaggtc acgcagtcgt
     3841 ggcatcgtcg atctctcggc ggagagtccc cggcgcggaa aacgggccgg gacctttgtc
     3901 agcgctcggc gagggggacg tagtcccggg gcgtcgcgcc ctggtagagc tgtcgcggtc
     3961 gtccgatctt gtaaggcgcg ccgatcatct cgttccaatg ggcaatccag ccaggggtgc
     4021 ggccggtggc gaagataacg gtgaacatct cggtgggaat gccgatggcc ttgaggatga
     4081 tccccgagta gaagtcgacg ttggggtaca gcttgcgctc ga
//
LOCUS       DBNHKV010000089        12064 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1501569, whole genome shotgun sequence.
ACCESSION   DBNHKV010000089 DBNHKV010000000
VERSION     DBNHKV010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12064)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12064)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12064
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1501569"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2689)
                     /gene="purL"
                     /locus_tag="AC16PP_02855"
                     /gene_synonym="purI"
     CDS             complement(<1..2689)
                     /gene="purL"
                     /locus_tag="AC16PP_02855"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019470323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HIR6480592.1"
                     /translation="MLTLEGPPARSAFRLNKLSGQLGLPEGALYAQYVHLLAVHQPLA
                     GQALERAQALLRYGPETGLPAPTGDLVATVVPRAGTISPWSSKATDIFAICGLDQVTR
                     VERGVRWFADRGLSDVQLALLYDRMTERAVGEADFPSVFANLPPKPMSTVALQAGGLA
                     ALEEANRALGLALSADEIEYLAGAYLELGRDPNDVELMMFAQANSEHCRHKIFNASWS
                     IAGEPQPLSLFEMIRNTYRSINGAGILSAYSDNAAVIEGHRIERFGVDSASHEYGYDE
                     AAVHVLMKVETHNHPTAIAPYPGAATGSGGEIRDEGAVGRGSKPKAGLTGFTTSHLNI
                     PGYGQPWELETGKPEHMVSALDIMVEGPVGAAAFNNEFGRPALAGYFRTFEQTVAGDP
                     RQVRGYHKPVMIAGGVGSIRPEHVQPTAFPAATALIVLGGPAMLIGLGGGAASSMGSG
                     ESSSDLDFASVQRDNAEIERRCQEVIDRCCALGERNPILLIHDVGAGGLSNALPELVK
                     DAGVGGHFQLRDVPNADPGMTPLEIWCNEAQERYVLAIAEEHLPGFAEICHRERCPFA
                     VVGRAVADPHLTVDDAEFANSPVDLPLSVLFGKPPKMQRSFAPRALTPPGFSHNGLVL
                     AEAIDRVLRFPCVASKQFLITIGDRSITGLVAREQMVGPWQVPCADVAVTLSGYRSYA
                     GEAFAMGERSPTALIDPAASARMAVGEALTNLCAAPVGDLGRAVLSANWMAAAGADAE
                     EQALFEGVRAVGMELCPALGIAIPVGKDSLSMRTRWDDREVTSPLTLIVSAFAPVTDA
                     RRTLTPELKTTPDTRLLLVDLGQGRNRLGGSVLAQTYGQLGAEAPDVNSPDMLGAFFR
                     AIQELNEQGMLLAYHDRSDGGLIACVLEMAFAGRCGLDLR"
     gene            complement(2717..3163)
                     /gene="tadA"
                     /locus_tag="AC16PP_02860"
     CDS             complement(2717..3163)
                     /gene="tadA"
                     /locus_tag="AC16PP_02860"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017131735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR6480593.1"
                     /translation="MSHVDWMRLALEEAGRAAAAGEVPVGAVVVRDGELLGSGFNCPI
                     SSSDPTAHAEIVALRAAAKRSGNYRLAGAAMYVTVEPCTMCAGALVHARIATLVYGAL
                     EPRSGAVTSTATVLENPLLNHRVEVVGGVLARECAEVLRAFFAERR"
     gene            complement(3188..4765)
                     /gene="guaA"
                     /locus_tag="AC16PP_02865"
     CDS             complement(3188..4765)
                     /gene="guaA"
                     /locus_tag="AC16PP_02865"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR6480594.1"
                     /translation="MADKYDIHDQRLLIIDFGAQYTQLIARRVREAGVYCEIHPFDVP
                     EDFIREFAPRGVILSGGPESVTFADTPRIPSIVFELGVPILGICYGMQAMAEQLGGSV
                     EASDVHEFGHAEIEIVETAELLGTVRDTAGGHLPVWMSHGDRVVRTPPGFEVTASTPS
                     APVAAMEHREKRMYGVQFHPEVTHTQRGEEMLAHFARTLCRCDGLWTAANIVEDAIAH
                     ARDLVGDERVVLGLSGGVDSSVVAALLSRAIGDQLTCVFVDNGLLRKGEREEVERAFA
                     PTNALALNLVSVPAAEEFLGRLAGVVDPEAKRKVIGNTFIEVFDREAHKISGVSWLAQ
                     GTIYPDVIESAAGKYGKAHVIKSHHNVGGLPERMHLKLVEPIRELFKDEVRKIGMHLG
                     LPRELVYRHPFPGPGLGVRVLGEVTAARADVLREADAIYLEELRAAGMYDAVSQAFAV
                     LLPVKSVGVVGDARRYADVIALRAVETTDFMTARCARLPYELIERVSNRIINEIPHVS
                     RVVYDVSSKPPATIEWE"
     gene            complement(4766..6235)
                     /gene="guaB"
                     /locus_tag="AC16PP_02870"
     CDS             complement(4766..6235)
                     /gene="guaB"
                     /locus_tag="AC16PP_02870"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR6480595.1"
                     /translation="MLRIAHDALTFDDVLLLPAYSQCLPSEVSLATKLTRDIHLNIPL
                     MSAAMDTVTEARLAIAIAQEGGVGIVHKNMDIESQAREVRAVKKHESGIIRDPITIHP
                     DKTVADLIELTMEHKISGVPVVDGRRLVGIVTGRDVRFERRMDAKVRDVMTTKEHLVT
                     ADEEASFEKITDLLHKHRIEKVLLINDRFELKGMVTVKDITKAQAYPNACKDHRGQLR
                     VGASVGTSPDTDDRVAALVEAGVDVLVVDTAHGHSQRVLDRISWIKQRYPDVNVIGGN
                     VATADGAKALLSAGVDAVKVGIGPGSICTTRMVTGIGVPQITAIAEAVAVADSEGVPV
                     IADGGVRYSGDIAKAIAAGASVVMVGGLLAGTDEAPGEVELYQGRTYKSYRGMGSLGA
                     MTESNGSSDRYFQEAEGDHRKLVPEGIEGRVPYRGPVGPIVHQLMGGLRASMGYTGSN
                     DIETMRTQPRFVRVSAASMVESHVHDVAITKEAPNYPVS"
     gene            6294..7685
                     /gene="xseA"
                     /locus_tag="AC16PP_02875"
     CDS             6294..7685
                     /gene="xseA"
                     /locus_tag="AC16PP_02875"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR6480596.1"
                     /translation="MLASQADRPVYSVSELAHEARILIEERFDLVWVEGEISNFRRPA
                     SGHWYFTLKDRSAQVRCAMFAGRNRQVRFAPEDGMQVLIRGRVSLYEPRGDFQVIVEH
                     AEPAGEGALRQAFEALKTKLAEEGLFAIERKRPLPALPRHLAIVSSASGAALQDVLHV
                     IERRFPALTVTLLPVSVQGNEAEAQIRRALARVDQVGADVVLITRGGGSLEDLWAFNL
                     ESVARAVAACPIPTVVAVGHQTDFVIAEFVADLRAPTPSAAAELITPDAAELAGRVGL
                     LQQRLVRGLSADVRLRQALVGRLRAQLVDPRQMVTRSMQRADDLEERLRLALGNRLER
                     NRNRLWALQRSLRAFRPEPLLRQQAARVTATRHALGKSLQRALAGSAERLGSAARTLD
                     AVSPLHTLARGYAVLAEPAADGLGRPVTAIAQVAPGATIDAHLHDGRLRVEVVSVNEE
                     SALQAAQTHVEEH"
     gene            7686..8486
                     /locus_tag="AC16PP_02880"
     CDS             7686..8486
                     /locus_tag="AC16PP_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR6480597.1"
                     /translation="MLARAALATLCWLTPMLLRADAVPGGLYAYGPVPPASEVRFDDR
                     RVLVIDETALVGIPLSASPGTHSLTITYASGESVSHQFEVVAKQYPAQRLTITDRKMV
                     NPDPEDLERIRTESARMREQYLSFTEALQRLRPFHKPVNGVTSSPFGRRRILNDQPRN
                     PHSGLDIAADHGTPVSAPAPARVSLTGDFYFNGKTIFLDHGQGLVTMYCHLSEISVEP
                     GEAVERGATIGLVGATGRATGPHLHWSVSLNGNRVDPELVISALAVGD"
     gene            complement(8524..9930)
                     /gene="der"
                     /locus_tag="AC16PP_02885"
     CDS             complement(8524..9930)
                     /gene="der"
                     /locus_tag="AC16PP_02885"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR6480598.1"
                     /translation="MVPTVALVGRPNVGKSTLFNKLTRRRDALVADVPGLTRDRRYGR
                     MPAGAGECMLIDTGGLFGEPGALQSALEQQAELALDEADAVLFVTDARDGLVPADEEI
                     AQRLRRLSKPVILVVNKMDGVQADVAMADFAALGFDAVAYVSAVHERGLGTVRELVEE
                     RCPHGPTGEEAADESDAVRVAVIGRPNVGKSTLVNRMLGQERQVVFDEPGTTRDAIEV
                     PFEKDGKRYVLVDTAGVRRKGRVEGVAEKFSVVKTLQAIDLAHVVILVLDAREGVVDQ
                     DLHLLSHAAEAGSGLVVAMNKWDGLSVEERTNASRGLDRKLSLAPGMPVMQISALHGT
                     GVGHLLEEVDRVYKAGELDVSTSVLTRLLLGMTGAHPPPAVRGRSIKLRYAHKAGSHP
                     PRIVIHGNQTEAVPDSYRRYLENGFRKALALVGNPVHIELRTGDNPYAGQRNELTPRQ
                     KARRKRMIQHRKRKAKKR"
     gene            complement(9938..11164)
                     /gene="bamB"
                     /locus_tag="AC16PP_02890"
                     /gene_synonym="yfgL"
     CDS             complement(9938..11164)
                     /gene="bamB"
                     /locus_tag="AC16PP_02890"
                     /gene_synonym="yfgL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HIR6480599.1"
                     /translation="MRKILLLLLTFSLTGCSWFSWLPWVGDKDDEKKKLEPAKLVDFD
                     AEVNVDRVWRASIGEGLGKKYLRLDPLVLADRVYAADGYGIVEALDRFSGKRVWRSRI
                     GRVEEGLLGSINFFDREDPSFVSGGVGGGAGYVFMGTTGGDVVALSAADGSEAWRSHV
                     GSEVLAPPVGERDLVFVQTQDGRLLALESETGDIRWSFDNQVPVLTLRGTGTPVVAGN
                     VVFAGFSNGMLAALRAESGEPVWEQRIMLPEGRSELERMVDVDGTPLLLGPILYVVAY
                     QGKLKALRRTDGAMLWEMDVSSFLDLAEGYGQIYVVDDGDVVTAVDQQSAEIVWQQPG
                     LARRKLTSPVAFSNYVAVGDDQGYLHILAQSDGRFLGRRKLDGDGLRSSMAVVDGVLY
                     VLGNSGSLHALDIEVK"
     gene            complement(11157..11858)
                     /locus_tag="AC16PP_02895"
     CDS             complement(11157..11858)
                     /locus_tag="AC16PP_02895"
                     /inference="COORDINATES: protein motif:HMM:NF021492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0044877 - protein-containing complex
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HIR6480600.1"
                     /translation="MSDYLTDEEQVAKLKSWWDENGVSVIVTLVLVVAGVVGWRWYES
                     YHETRVARVSDLYEEFLVSEGDARDASAASIAEEAPDSAYQTLVMLRRSQESMESGDY
                     EAAEAFLVEAVEAAPEPVLADVARVRLARVQQQLDRLDDALETLSGVLGEGYRSQVAE
                     MKGDIHLARGERALAYEAYASALAAVGDNQSRPVLEMKAADTADAVTGAQPAAAPEAG
                     QGLESPGETAETPDA"
     gene            complement(11890..12051)
                     /locus_tag="AC16PP_02900"
     CDS             complement(11890..12051)
                     /locus_tag="AC16PP_02900"
                     /inference="COORDINATES: protein motif:HMM:NF015113.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6480601.1"
                     /translation="MRRADASGARLAVLVGEDEIAKGVVSLKYLRESREQESVPAADL
                     ETVLAEYFA"
BASE COUNT         2080 a         4201 c         3748 g         2035 t
ORIGIN      
        1 cgcgcagatc cagcccgcag cgcccggcga aggccatttc cagcacgcag gcaatgagac
       61 cgccatccga acggtcgtga taggccagca gcatgccctg ctcgttcagt tcttggatcg
      121 cacggaaaaa ggccccaagc atatcgggcg agttcacatc gggagcttcc gccccgagct
      181 gaccataggt ctgggcaagc acgctgccgc ccagccggtt tctgccctga ccgagatcca
      241 ccagcaggag gcgggtatcg ggggtggtct tgagctcggg cgtgagggta cggcgcgcat
      301 ccgtcaccgg cgcaaacgca gagacgatga gcgtcagcgg cgacgtcact tccctgtcgt
      361 cccaacgagt gcgcatggaa aggctgtcct tgcccaccgg tatggcaatt ccgagggccg
      421 gacagagttc catccccacc gcccgtacgc cttcgaacag cgcctgctct tctgcatcgg
      481 cccccgcggc ggccatccag tttgccgaca gcaccgccct gccaagatcg ccgacaggcg
      541 ccgcacacag gttggtcaag gcttcgccca cggccattcg agcggatgcg gcgggatcga
      601 tgagcgcagt cggtgaccgc tcgcccatgg caaacgcctc gccggcatag ctccggtatc
      661 cggatagcgt gaccgcaaca tccgcgcacg gcacctgcca cgggcccacc atctgctccc
      721 gcgccaccag cccggtgatg ctccgatcgc cgatggtgat cagaaactgc ttgcttgcca
      781 cacacggaaa gcgcaaaacc cggtcgatgg cctctgcgag aacgagacca ttgtggctga
      841 acccaggcgg ggtgagcgcc cgcggtgcga acgaacgctg catcttaggc ggcttgccga
      901 acagcacgga aagcggcagg tccacgggcg agttggcgaa ctcggcatca tcgaccgtca
      961 ggtgcgggtc ggccacggcc cggcccacga cggcaaaggg gcaacgctcg cgatggcaga
     1021 tctcggcgaa ccccggcaga tgctcttcgg caatcgccag cacgtagcgt tcctgtgcct
     1081 cgttgcacca gatttccaga ggcgtcatgc ccgggtccgc gttgggcacg tcacgcagct
     1141 gaaaatgccc gccgacgccc gcgtccttga ccagctccgg aagggcattg gacaacccgc
     1201 cggcgccgac atcgtgaatg aggagaatgg gatttcgctc tcctaaagcg cagcagcgat
     1261 cgatcacctc ctggcagcgg cgctcgatct ccgcgttgtc ccgctgaacc gaggcaaaat
     1321 cgaggtcgga cgagctctct cccgatccca tgctcgaggc ggcgcccccg ccgagcccga
     1381 tgagcatggc cgggccaccg agcacgatca gcgcggtcgc cgccgggaaa gcggtgggtt
     1441 ggacgtgctc cgggcggatg ctgccgacgc cgccggcgat catgacgggc ttgtgatagc
     1501 cgcgaacctg gcgggggtcg ccggcaacgg tctgctcgaa agtacggaaa tagccggcca
     1561 gagccggtct tccgaactcg ttgttgaagg cggccgcacc cacggggcct tccaccatga
     1621 tgtccagcgc gctcaccatg tgttccggtt tgccggtctc gagctcccag ggctggccat
     1681 agccggggat gttgaggtgc gaggtcgtga agcccgtaag gcccgccttc ggcttcgacc
     1741 ctcggcccac ggcgccctca tcgcggatct cgccgccgga cccggtggcg gcccccgggt
     1801 acggggcgat ggccgtcggg tgattgtggg tctctacctt catcaacacg tggacggctg
     1861 cttcgtcgta gccgtactcg tgagacgcgg agtccacacc gaaacgctcg atgcgatgcc
     1921 cctcgatgac ggctgcgttg tcggaatagg cgctgagaat cccggccccg ttgatcgacc
     1981 ggtaggtatt cctgatcatc tcgaacagcg aaagcggctg aggttcgccg gcgatgctcc
     2041 acgacgcgtt gaatatcttg tggcggcagt gctccgagtt cgcctgagcg aacatcatca
     2101 attcgacgtc gttcgggtca cgcccgagct ccagataggc gcccgcgagg tattcgatct
     2161 catccgcgga cagcgcaagg cccaacgccc ggttggcctc ttccagcgcc gccaggccgc
     2221 ccgcctgcag agcaaccgtg ctcatgggct ttggcggcag gttcgcgaaa accgagggga
     2281 agtccgcctc gccaaccgcc cgctcggtca tgcggtcgta aagcagcgca agctggacat
     2341 cgctcaggcc cctgtccgca aaccaccgca cgccccgttc gacccgggtc acctggtcca
     2401 gaccgcagat ggcgaagatg tcggtcgcct tgctggacca cggcgagatc gtgccggccc
     2461 gaggaaccac ggttgcgacc agatcacccg tcggcgccgg cagccccgtt tccggaccgt
     2521 agcgaagcag cgcctgcgcg cgctccagcg cctgtcccgc aagcggctga tggacggcca
     2581 ggagatggac gtactgagcg tacagcgcac cttccggcag gcccagctgc ccgctcagct
     2641 tgttcaggcg aaaggcggag cgggccggcg gcccttcgag cgtcaaaatc tccatgaacc
     2701 gatcctcatc cgatgctcac cgccgctcgg caaagaacgc gcgcaaaacc tccgcgcact
     2761 cgcgcgcaag cacgccgccc accacctcga cccggtgatt cagcaacggg ttttccagaa
     2821 cggtcgccgt gctggtcacc gcaccggatc gcggctccaa cgcgccgtag accagggtcg
     2881 cgatccgcgc gtggacgagg gctccggcac acatggtgca cggttccacc gtcacgtaca
     2941 tcgcagcacc cgcaagccgg tagttgccgg accgtttagc ggcagcacgc agcgcaacga
     3001 tctccgcatg tgccgtgggg tcgctgctgc tgatgggaca gttgaagcca ctccccagca
     3061 gctccccgtc ccgaaccacc accgcgccca cgggcacctc tcccgcggcg gctgcgcgac
     3121 ccgcctcttc tagagccagg cgcatccagt caacgtggct catcgccctc ccgtgccaac
     3181 cacacggtca ctcccactcg atggtcgctg gcggtttgct ggagacgtcg taaaccaccc
     3241 gcgaaacgtg ggggatctca ttgatgatcc ggttggatac acgctcgatc agctcgtagg
     3301 gcaaccgtgc acagcgggcg gtcatgaagt ccgtggtctc caccgccctc agggcgatga
     3361 cgtccgcgta acgccgtgcg tcgcctacca cgcccaccga cttcaccggc agcagcacgg
     3421 caaacgcctg gctgacggcg tcgtacattc ccgcagctcg gagctcttcg aggtagatgg
     3481 cgtccgcttc gcgcaggacg tcggcacggg cggcagtcac ctcgccgagc acccgcacgc
     3541 ccaggcccgg accgggaaac gggtgccggt agaccagctc ccgaggcagc cccagatgca
     3601 tcccgatctt gcggacctcg tccttgaaca gctcgcgaat cggctccacc agcttcagat
     3661 gcatccgttc ggggaggccg ccgacgttgt gatgggactt gatgacatga gccttgccgt
     3721 acttgcccgc tgccgactcg atgacgtcgg gataaatggt gccctgagcc agccagctca
     3781 ccccggagat cttgtgcgct tcacgatcga agacctcgat gaacgtatta ccgatgacct
     3841 tgcgtttcgc ctcggggtcc acgacaccgg ccaggcgccc cagaaactct tccgcggccg
     3901 gcacgctgac caggttgagc gccagagcgt tggtcggcgc aaaagcacgc tccacctctt
     3961 cccgctcgcc cttgcgaagc aggccgttgt ccacgaacac gcacgtcagc tgatccccga
     4021 tggcccggga gagcagagca gcgaccacgc tggaatccac gcctccggac aaccccagca
     4081 cgaccctttc gtctccaacc aggtcgcggg cgtgggcgat ggcgtcctcg acgatgttgg
     4141 cggccgtcca caggccatcg cagcgacaca gcgtccgcgc gaagtgggcg agcatctctt
     4201 cgccccgctg ggtgtgcgtc acctcgggat gaaactgcac gccgtacatg cgcttttcgc
     4261 gatgctccat cgcagccacg ggcgccgacg gggtgctggc ggtgacctcg aagcccggag
     4321 gcgtcctcac cacccggtcg ccgtggctca tccagaccgg cagatgcccg ccggcggtgt
     4381 cacgcaccgt gccgagcagc tcggccgtct cgacaatttc gatttctgcg tggccgaact
     4441 catggacgtc ggacgcttcc acggaaccgc ccagctgctc tgccatggcc tgcatgccgt
     4501 aacagatgcc gaggatgggc acccccagct cgaacacgat ggacgggatg cgcggcgtgt
     4561 ccgcaaacgt taccgactcc gggccgccag agaggatcac cccccgcggc gcgaactcgc
     4621 gtatgaaatc ctccgggacg tcgaaggggt gaatctcgca ataaacgccg gcctcgcgca
     4681 cccggcgggc aatcagctgg gtgtactggg caccgaaatc gatgatcagg aggcgctgat
     4741 catgtatgtc gtatttgtcc gccattcagc tgacgggata gttcggcgct tctttggtga
     4801 tcgccacgtc gtgaacgtga ctctccacca tgctggccgc gctcactcgc acgaagcgag
     4861 gctgggttcg catggtctcg atatcgttcg agccggtata gcccatcgac gccctgagcc
     4921 cgcccatgag ctgatgaacg atggggccca caggcccacg gtaaggcacg cgcccttcga
     4981 tcccctcggg aacgagcttg cgatgatccc cctctgcttc ctggaagtac cgatcgctgg
     5041 acccgttgga ctcggtcatc gcacccagcg agcccatgcc gcgataggac ttgtaggttc
     5101 tgccctgata gagctctacc tctccgggcg cttcatcggt gccggcgagc agcccgccga
     5161 ccatcaccac gctggccccg gccgcgatgg ctttggcaat gtcgcccgag taacggacac
     5221 ctccatcggc aatgacgggc accccttccg agtcggcaac cgccaccgct tcggcgatgg
     5281 ccgtgatctg cggaacacca atgccggtaa ccattcgcgt ggtgcagatg gagccggggc
     5341 cgatgccgac cttgaccgca tccacgccgg cggaaagaag cgctttcgcg ccgtccgcgg
     5401 tagccacgtt gccgccgatg acgttgacgt ccggatagcg ctgcttgatc cagctgatgc
     5461 ggtccagcac ccgctgggag tgcccgtgag ccgtatccac caccagcacg tcgacgccgg
     5521 cttccacgag ggccgcgacc cggtcgtcgg tgtcggggct ggtacccacg ctcgcaccga
     5581 cccggagctg tccccggtgg tctttgcagg cattgggata agcctgggcc ttggtgatgt
     5641 ccttgacggt caccatgcct ttgagctcga agcgatcgtt gatgaggagg actttctcga
     5701 tgcgatgctt gtgaaggaga tcggtgatct tctcgaagct tgcctcctcg tccgcggtga
     5761 cgaggtgctc ctttgtggtc atcacgtcgc ggaccttggc gtccatgcga cgctcgaacc
     5821 gcacgtcgcg tcccgtgacg atgccgacca gccggcgtcc atccaccacc ggcacgccgg
     5881 agatcttgtg ttccatggtc agctcgatca gatccgcgac cgttttgtcc ggatggatgg
     5941 tgatcgggtc acggatgatg ccactttcgt gcttcttgac cgcccggact tcacgggcct
     6001 ggctctcgat gtccatgttc ttgtgcacga tgccgacccc gccctcctgc gcgatcgcaa
     6061 tagcaagccg agcctccgtc accgtgtcca tggcagcgga catgagcggg atattgaggt
     6121 ggatgtctct ggtcagcttc gtcgcgagcg aaacttctga aggcaagcac tgggagtagg
     6181 cgggaaggag cagaacgtcg tcgaacgtga gcgcgtcatg cgcaatacgc aacattggtc
     6241 actccgcaaa agcggcattc tatcggcgca ccccgatcat gtaaactcga cccatgctcg
     6301 ccagccaagc agatcgcccc gtctacagcg tcagcgaact cgcccacgag gcgagaatcc
     6361 tcatcgagga gcgcttcgac ctggtctggg tggagggcga gatctccaac ttccggcgtc
     6421 ccgcatccgg ccactggtac tttacgctca aggatcgctc cgcccaggtt cgctgcgcga
     6481 tgttcgccgg ccgcaaccgc caggtacgct tcgctcccga ggacggcatg caggtcctga
     6541 tcaggggccg tgtcagcctt tacgagccgc gcggcgactt ccaggtcatc gtcgagcacg
     6601 cggagcccgc cggcgaaggc gcgctgcgcc aggccttcga ggccctgaag accaagctcg
     6661 cagaggaagg tctcttcgcg attgagcgta agcggccgct gcccgcgctg ccccgccacc
     6721 tggcaatcgt ctcgtcggcc tccggcgcgg ctctccagga cgtcctccac gtcatcgagc
     6781 ggcgctttcc ggcgctgacc gtcacgctgc tgccggtcag cgtgcagggg aacgaggccg
     6841 aggcccagat ccggcgcgcg ctggcgcggg tcgatcaggt cggcgccgac gtggtgctga
     6901 tcacgcgcgg cggagggtct ttggaggatc tctgggcgtt caacctcgaa agcgtcgccc
     6961 gcgcggtggc agcgtgcccg atacccacgg tggtggccgt gggccaccag accgacttcg
     7021 tcatcgccga gtttgtggcc gacctgcgcg cacccacgcc atccgccgcc gccgagctca
     7081 tcacgccgga tgccgcagag ctggccgggc gcgttgggct gcttcaacag cgactggtcc
     7141 gcgggctttc cgccgacgtg cgcttgcggc aggcgctggt ggggcggctg cgagcccagc
     7201 tcgtcgatcc gcgccagatg gtgacccgca gcatgcagcg tgcggacgat ctggaggagc
     7261 gcctgcggct ggccctcggc aaccggctgg agcggaaccg aaaccggctg tgggcattgc
     7321 agcgcagcct ccgggccttc cgtccggagc ccctgttgcg ccagcaagcg gccagggtca
     7381 ccgccacgcg tcacgccctg gggaaatccc ttcaacgcgc cctggcgggc tccgcggagc
     7441 gcctcggatc ggccgcgcgc acgctggatg ccgtcagtcc tctgcacacg ctggcgcgtg
     7501 gctatgccgt gctcgccgag cccgcagccg atgggctcgg acggccggtg accgccatcg
     7561 cccaggtcgc ccctggtgcg accatcgatg ctcatctgca cgacggccgg ctccgggtgg
     7621 aggtggtgtc cgtgaacgaa gaaagcgcgc tgcaggcggc gcaaacccac gtggaggagc
     7681 actagatgct cgcccgcgcc gcgctggcca cgctgtgctg gctgacgccg atgctcctgc
     7741 gggccgacgc ggtgcctggc ggtttgtacg cctacgggcc cgtgccgccc gcctcggagg
     7801 tgcgtttcga cgatcgtcgg gttctggtta tcgacgagac cgctctcgtg ggcatcccgc
     7861 tgtctgcatc acccggcacg cactcgctga ccatcaccta tgcgtccgga gagtcggtct
     7921 cgcaccagtt cgaggtggtg gcaaagcagt acccggcgca gcgcctgact atcaccgatc
     7981 ggaaaatggt caatccggac ccggaggacc tcgagcgcat acggacagaa agcgcgagga
     8041 tgcgcgagca gtacctgagc tttaccgaag ccctgcagag gctgcgtccg ttccataagc
     8101 cggtgaacgg agtgacctca agccccttcg gccgccggcg catcctcaac gaccagcccc
     8161 gcaacccgca cagcggtctg gacatcgccg ccgaccatgg cacccccgtg agcgctccgg
     8221 cacctgcaag ggtcagtctc acgggcgact tctatttcaa cggcaagacg atcttcctcg
     8281 atcacggaca gggactggtg accatgtact gccacctgag cgaaatcagc gtggagcccg
     8341 gtgaggcggt ggaaaggggc gccaccatag gtctggtggg cgccaccggg cgggccacgg
     8401 gaccccacct gcactggtcg gtcagcctaa acggtaaccg ggtcgatccc gagctggtaa
     8461 tctctgccct ggccgtcggg gactagccca ccttcggcac caccgggcgg gagaggacgc
     8521 cggtcagcgt ttttttgctt tgcgcttgcg atgctgaatc atccttttgc gacgggcttt
     8581 ctgacggggc gtgagctcgt tacgctggcc ggcataagga ttgtctccgg tgcgcagctc
     8641 gatgtgcacg gggttgccca ccagggcgag agccttccgg aagccgtttt ccaggtatct
     8701 tcgatagctg tccggaaccg cttcggtctg gttgccgtgg atcacgatcc gggggggatg
     8761 cgatccggcc ttgtgggcgt agcgcagctt gatggaacgg ccacggactg cgggtggcgg
     8821 gtgggcgccc gtcatcccga ggagcagccg ggtcagcaca gacgtgctga cgtcgagctc
     8881 tcccgctttg tagacccggt cgacctcttc gagaaggtgg cccacgccgg tgccgtgcag
     8941 ggccgagatc tgcatcaccg gcatcccggg tgccaaagac agcttccggt ccagaccccg
     9001 gctggcattc gtcctttcct cgaccgagag cccgtcccac ttgttcatgg ccaccaccag
     9061 ccccgagccc gcctcggcgg catggctgag caggtgcaga tcctgatcca ccaccccttc
     9121 ccgggcgtcg aggacgagta tgaccacgtg ggccagatcg atggcctgca gggtcttcac
     9181 caccgagaac ttctcggcca ccccctcgac ccgccctttg cgccgcacgc cggcggtatc
     9241 caccagaacg tagcgcttgc cgtccttctc gaaaggcacc tcgatggcgt cacgcgtggt
     9301 gccgggttca tcgaacacca cctggcgctc ctggccgagc atgcggttca ccagcgtgga
     9361 tttccccacg ttggggcggc caatcaccgc gacgcgcacg gcatccgatt cgtcggctgc
     9421 ttcctcccca gtcgggccgt gggggcaccg ctcctccacc agctcgcgta cggtgcccaa
     9481 tcccctctcg tgcaccgcag agacataggc cacggcatcg aagccgaggg cggcaaagtc
     9541 ggccatcgca acgtccgcct gcacgccatc catcttgttg accaccagaa tcaccggttt
     9601 ggaaagacgc ctcaggcgtt gggcgatctc ctcgtcagcg ggcaccaggc cgtcccgggc
     9661 atcggtcacg aacagcaccg cgtcggcttc gtcgagtgcc aactcggcct gctgctcgag
     9721 ggccgactgc agcgcgcccg gttccccgaa gagccccccg gtgtcgatga gcatgcactc
     9781 accggcccct gccggcatgc gcccgtagcg ccgatcccgg gtcaggccgg gaacgtcggc
     9841 caccagagcg tcccggcgcc tggtcagctt gttgaagagt gtggacttac cgacgttggg
     9901 ccggccaacg agggcgacgg tgggaaccat cccgggccta tttgacctca atgtcgagcg
     9961 catggaggga accggagttt ccgagcacgt aaagcacgcc gtccacgacc gccatcgacg
    10021 accgcagccc gtccccgtcg agcttgcggc gcccgaggaa gcgcccgtca ctctgcgcaa
    10081 ggatgtgcag atatccctga tcgtcgccca ccgcgacata gttgctgaaa gcaacggggc
    10141 tcgtgagctt acgacgcgca aggcccggct gctgccagac gatctccgcc gactgctggt
    10201 cgacggccgt caccacgtcc ccgtcgtcca ccacgtagat ctggccgtat ccttcggcga
    10261 gatccaggaa gctggacacg tccatctccc acagcatggc tccgtcggtt cgtcgcagcg
    10321 ccttgagctt accctggtaa gccacgacgt agaggatcgg acccaggagc aggggcgtgc
    10381 cgtccacgtc caccatgcgt tccagctcgg accttccttc cggaagcatg atccgctgct
    10441 cccagacggg ctcgccgctt tcggcgcgca gagcggcgag catcccgttg gaaaagcccg
    10501 caaacaccac gttaccggcc accaccgggg tgccggtgcc tctcaaggtc agcaccggaa
    10561 cctggttgtc gaagctccag cgaatgtccc cggtctcgga ttccagcgcc agcagcctgc
    10621 cgtcctgagt ctgcacgaac accagatcgc gctcgcccac cggtggcgcg agcacctcgc
    10681 tgcccacgtg ggaccgccag gcttccgaac cgtccgcagc ggacagcgcc accacatcac
    10741 cgcccgtcgt gcccatgaaa acgtagcctg caccaccgcc gaccccgcca ctcacgaaac
    10801 tcggatcttc acgatcgaag aagttgattg agcccaggag accttcttcg acgcgaccta
    10861 tgcgcgaccg ccagacccgc ttgccggaaa aacggtcgag cgcttccacg atgccatagc
    10921 cgtccgcggc gtacacccgg tcggcgagga ccaggggatc aagtcgcaga tacttcttgc
    10981 cgagaccctc accgatggag gcccgccaga cccggtcgac gttcacctcg gcatcgaagt
    11041 ccaccagctt ggccggctcg agctttttct tctcgtcgtc cttgtcgccc acccacggca
    11101 accaggaaaa ccagctgcag ccggtaaggg agaaagtcag caggagcagt agaattttac
    11161 gcatcgggcg tttctgcagt ctccccgggg gattcgaggc cctgtccggc ttccggcgct
    11221 gcagcgggct gcgctccggt cacggcatcg gcggtatcgg cggctttcat ctccagaacc
    11281 ggacgggact ggttgtcacc caccgccgca agcgccgaag cgtatgcttc gtaagcaagg
    11341 gcgcgctccc cgcgggccag atggatatcc cccttcatct cggcaacctg tgaccgatat
    11401 ccttcgccaa gcaccccgga aagcgtttcg agcgcatcat cgagacggtc cagctgctgc
    11461 tgcacccgcg cgagccgcac ccgggcaacg tccgccagaa cgggttccgg agcagcttcc
    11521 accgcctcga cgaggaacgc ctcggcggct tcgtagtcgc cgctctccat ggactcttgc
    11581 gagcggcgca gcatgacgag cgtctgatag gcactgtcgg gtgcctcttc ggcgatggac
    11641 gcggcgcttg cgtccctggc gtccccttcg ctgaccagaa actcctcgta gagatccgaa
    11701 actcgcgcga cccgcgtctc atggtagctt tcgtaccatc gccaaccgac caccccggcg
    11761 acgaccagaa cgagcgtcac gatgacggac acgccgttct cgtcccacca tgatttgagc
    11821 tttgcgacct gctcttcgtc ggtcaggtaa tccgacaccc caatctcctt ttcgattcgc
    11881 tcgccaggtt caggcgaagt actcggccag caccgtttcc agatcagcgg ccgggacgct
    11941 ttcctgctcc cggctctcgc gaaggtattt cagcgacacc acgcccttgg cgatctcgtc
    12001 ctcgccgacg agaaccgcga ggcgcgcgcc gctggcatcg gcgcgtcgca tctggctctt
    12061 cagc
//
LOCUS       DBNHKV010000090        14856 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_155656, whole genome shotgun sequence.
ACCESSION   DBNHKV010000090 DBNHKV010000000
VERSION     DBNHKV010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 14856)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14856)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14856
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_155656"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1513
                     /gene="edd"
                     /locus_tag="AC16PP_02905"
     CDS             <1..1513
                     /gene="edd"
                     /locus_tag="AC16PP_02905"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HIR6480602.1"
                     /translation="GAVAQVAGGVPAMCDGITQGRPGMELSLFSRDVIAMSTALALSH
                     DMFDAVVCLGVCDKIVPGLLMGALAFGHLPAVFVPAGPMPSGLPNKEKARVREAYAAG
                     EAGRVELIAAESAAYHSPGTCTFYGTANSNQMLLEFMGLQLPGGSFVNPGSELRDLLT
                     AAAVRQVLELTRLGNRHMPIGRIVDESSVVNAVVGLLASGGSTNHTMHLVAVAAAAGI
                     RLDWSDFADLSRVVPLMARVYPNGEADVNHFHDAGGLGFMIGELLDHGLLHDEVETIL
                     GRGLSRFVVCPERADGALRWRPAKKSSADPGVLRGVAEPFDAEGGLRVVQGDVGRAVV
                     KVSAVRAERRRISAPARVFVSQDAFAEAFEAEELEGDMVAVLPWQGPRANGMPELHRL
                     SPYLGVLERRGYRVALLTDGRMSGASGAVLAAIQVTPEAASGGLIGRIRDGDLIHIDA
                     DAGVVRVEADLEGRTGAAAEAPDGTHGTGRELFSAFRAQVGPAESGASIFGPL"
     gene            1515..2144
                     /gene="eda"
                     /locus_tag="AC16PP_02910"
     CDS             1515..2144
                     /gene="eda"
                     /locus_tag="AC16PP_02910"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - (R,S)-4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIR6480603.1"
                     /translation="MDACDLLDGVGIVAVVALEDADAAVPLARVLAGSGVSCVEVTLR
                     TGCAVDAIGRIAREVPEVLVGAGSIRRPEQLLEVRDAGARFAVSPGSSDGLLAASRRL
                     KMPFVPGAVTATEMIGLLEHGYFLQKFFPAEASGGARALRSLAAPLPEVRFFPTGGIT
                     QELAPGYLQMGSVACIGGSWFVPADSLRAGDFSAIGRLAGAAAELTTLS"
     gene            2187..3674
                     /locus_tag="AC16PP_02915"
     CDS             2187..3674
                     /locus_tag="AC16PP_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6480604.1"
                     /translation="MSSAEHYATAPEDRISFAHKVVYGFGAFVNNLLAAAIGGMAIVL
                     NLGLGMNPALVGLLGALPRLTDALTDPLMGYISDHTNTRWGRRRPYIFAGAIAAGLCF
                     ALLWQLPPGRSESFYFYYFLFGSLIFYLAYTVFATPWVALGYELTPDYHERTRLMGVQ
                     NFIGQLAYLISPWFLWMMQYEPVFDDMVEGAAGLSVAIAVVVMCVGVLPALLLRERFR
                     DVAHAEIAEKQLARGEPRHPLLTNVKSFFAGFAATLKFKPFVRLCAATFLVFNGFMLV
                     SSFQFYVIIYYVFSGDQVTGAEYAGWSGTLSAVSTFVVIGIVTWLATKIGKRRAFFAA
                     TGISIVGYGLKWFCYDPAVPWLLLVPAPLIAFGLGGLFTLMGSMIADVCDLDELASHE
                     RREGMFGSIFWWVVKLGMALALAGGGFLLNATGFDVALGGAQPASAILMMRAFDVIVP
                     MLSSAAAIWIIAGFPITEERAHQVRARLEERRGALNGDPGTAAAV"
     gene            3739..4770
                     /locus_tag="AC16PP_02920"
     CDS             3739..4770
                     /locus_tag="AC16PP_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6480605.1"
                     /translation="MRSQKSSQKVTIVDVAKRAGVSIKTVSRVLNSEPNVRADTREKV
                     LKAAKALHYQPNPSARGLAGNRSFSIGLLYENPQEFGYVKDALNGVIGTCEAEGYSLL
                     MRPCGEEVDSEEVRKFVAQTRVDGAVLTAPVCDEPKVTDLLKELGVPFAQLAPKAPRS
                     EWISVQCNDEEASYAMTDYLISLGHRRVGFIKGHPDHGATEKRLAGYRRCLADHDMPF
                     DKTLVMQGYFDFESGQRCARKLLELPNPPTAIFASNDDMAAGVIFTARERGIGVPDEL
                     SVAGFDDSPAASHTWPPLTTVKQPIVEMAARATQRLIGQLRGVGEEGDEEIFTCELIV
                     RRSTAKRGD"
     gene            4970..7405
                     /locus_tag="AC16PP_02925"
     CDS             4970..7405
                     /locus_tag="AC16PP_02925"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR6480606.1"
                     /translation="MTSGKPADSNFWPHCASPVGQDAATELRVADVLSRMTLEQKVGQ
                     MIQAEIAAVTPAQAAEHHLGSVLNGGGSFPNGDKHASVADWVALAESYRAASSDAGPG
                     RLGIPLIWGTDAVHGHNNVYRATIFPHNIGLGAAGDPDLIEEIGAATAREIAATGIGW
                     TFAPTLAVVRDDRWGRTYESYSEDPQIIRAYAGRMVTGLQGPAGTDAFLGAGRVVATA
                     KHFLGEGGTEGGVDQGENNADETTLRDVHGQGYVTALGSGAQTVMATFNSWRGVKVHG
                     HRYLLTDVLKGQMGFDGFVVSDWNGFKQVADCRQEACQLSINAGVDMLMIPDDWQEVI
                     GDLVALVEDGQIPMSRIDDAVTRILRVKARAGLLDPAESSDRPHTDPAVVGCAAHRDV
                     ARRAVRQSLVMLKNRGGMLPLDPSLHVLVAGDGADDIGKQSGGWTLTWQGTENENGDF
                     PGASSIYDGISAAVTNAGGRATLSPDGSWSERPDVAVVVFGEDPYAEGQGDRAHLSFS
                     ALFPEGLALLKKLKGDGIPVVSVFLTGRPLWVNPELNASDAFVVAWLPGSEGAGVSDV
                     LFRTVGGEVAHDFTGRLAFSWPRSSLQATVNRHDADYDPLFPYGFGLGAADVDTLGDE
                     LIEEDEALGLAADESIGILTGRVVPPFEFFIGDSQAWKVPVRGRSSRSPTGAVSVTNV
                     DHYSQEDARRISWDGSGGAQVYFQRSAGQDLSDHASRGARLRCKLRVDVRPQRRVELR
                     MDSGYPQTASIDITAMLNELPLGTWEDVAFEMSAFAGGTDNLCAVDTPWLIWTDGVLG
                     LSVSNLRIDLGDG"
     gene            7402..8733
                     /locus_tag="AC16PP_02930"
     CDS             7402..8733
                     /locus_tag="AC16PP_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HIR6480607.1"
                     /translation="MTSILGRFPAVLVLLSLADAGLVPEAGAMPDEPVKVEVVRTARG
                     YALERGGRPYRIQGVGGLERLALLAESGGNSIRTWSVGTPEQSARELLDEAHRLGLTV
                     ALCLDIARERHGFDYDDDAAVAAQFERARADVLAHRNHPALLAWIIGNELNHDFKNPR
                     VYDAVNEISRMIHEIDGNHPTTTTIAGFSADMAEVISARAPDIDFLSIQSYAGVLDLQ
                     RRVRKAGYEGPYVLTEWGPPGHWEVATTSWGAPIEPDSSAKAKALRRAHATAIEPYPD
                     QLIGNYVFLWGQKQERTPTWYGLFTETGEKTESVDVMHYLWTGTWPGNRSPAVRSVRL
                     DGRRAGRDIVLVPGQTYPILARVRDPEGDALRYRWSVKSESRARQVGGDLEGAIADVD
                     GLIDEPSSSRAQLTAPEEPGAYRVFVYAYDGNGGAAHANVPFLVRSPAGAD"
     gene            8827..10053
                     /locus_tag="AC16PP_02935"
     CDS             8827..10053
                     /locus_tag="AC16PP_02935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6480608.1"
                     /translation="MIDFEIPAETKAIRAKAREFVQKECVAAEAQCTADNFDQVLSEL
                     RGKARAQGLWCPFIPEEHGGMGLKPLANALVQMELGESFLGALALNTQGPDDATMLTL
                     LEHGTDHQKEKFLKPLLDGQKRICYSMTEKAAGADATGMQTAAVLEGDNWVLNGEKWF
                     SSSADVADIAVVMAKTDPDAPRHERYSTFIVELPNPGYQILRNIDTMQPHTELGLKLG
                     HSHSEVKIENLVVPRENLLGGRGKGFAMGQHRLAYGRLRHGMHNVAMAQRALDLAAGH
                     VVNRETFGKRLADRQGVRWMLADCAAEIYKARLMLLHIAYKAERGMDLRQENGIAKVF
                     LAHMVHQVVDTALQLHGALGYSHDTPLASWYTSIRSQRLVDGPDEVHRWRTGANVIKA
                     YEEHGTTAPACGGELL"
     gene            10050..11930
                     /locus_tag="AC16PP_02940"
     CDS             10050..11930
                     /locus_tag="AC16PP_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012946.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6480609.1"
                     /translation="MSEARTEPAVRVDPATGVKRFATRAAKATDKIAGKGYSVIADEA
                     LTTLPPAPPGAVFDAEEQAKYRAFKEVRRGAADYMTMEGEFARYLEDVYSADPVPREA
                     LTDECEVLVVGAGFAGLLLWQKLSAAGFTDVRFCEKGGDVGGTWYWNRYPGIACDVES
                     YSYLPLLEEMGYIPSMKFASGFEILEYCQRMAEKFGFYDRCLFHTTVDRTEWDEAAGR
                     WTVYTNRGDAMRARFVVLANGILTTPQLARIDGMETFKGESFHTSRWNYHVDLAGKRV
                     GIIGTGATAVQVIPEIAKIVKELYVFQRTPSSIDVRDQRETTPEEYETWSHEPGWARA
                     RRARFSKISSGRTAIKANDDYLSGKVADFKERKQHERRLSAEEMINKQLETNFRIMEQ
                     IRARVDAIVEDPRTAEALKPYYPYGCKRPTFHDEYLPTFNLPHVTLVDTAPLGVTRIN
                     ERGVVHDGREYPLDVLIYATGFQWMATSTFNMIRGRGGRTLNEKWQEEGTRTFLGIHS
                     HGFPNLFIVSGPQGGGGSFNFTDAIDTHADYICWMLETMRDRGARTVDVRKEPEDEYA
                     EHCRAADVMTAPLRDCISYYNGHGDAEPGSLAYYGGGRWHKYRVRAQESLEPYDFDDI
                     PE"
     gene            12030..13526
                     /locus_tag="AC16PP_02945"
     CDS             12030..13526
                     /locus_tag="AC16PP_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6480610.1"
                     /translation="MTDLVRKGWTVLALWALTMILGYFGLGGFLAETVEGIGGVPAWP
                     TALFVVLLAVSLWASVVWARGLKAKPEAGAALEERPLASGRRRFLTGTAAVTGGMLGA
                     AGATIGRVAPWATVTLPALRQPVTVTDPNPKDDWLGARVRSYRPLGDTGLMVSDIATG
                     STRIHHNSDPVGYLKSLLDRGVNYIDASPDYAPESEGIIAEAIVGRPRDQLVIASKFC
                     TRDGHLRQGSSVAAYMTAIDGTLSRMGTDYIDVAHIHSCDSTERLMDENVHEAFDRLK
                     DAGKVRHLGVSTHTPNLEEVADTAIDSGRFKVMMLAYHHGAWPKQMDIIDRAAAAGMG
                     IVAMKTLKGAKHRGLAEFRPEADSYAQAAFKWVLANPSVSNLVVSFYDNQHPDEYLFA
                     SGKSLERSDIAVLGKYDQLIAGTHCFQHCGDCLGACPSGLAINDILRHRMYFEDYGDE
                     KQAMQLYAKLDRQADVCIDCSAPCLGSCPQGVEIQERMIGAHEKLRWA"
     gene            complement(13558..14760)
                     /locus_tag="AC16PP_02950"
     CDS             complement(13558..14760)
                     /locus_tag="AC16PP_02950"
                     /inference="COORDINATES: protein motif:HMM:NF017984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6480611.1"
                     /translation="MPTVTDISTRDARRLFLHRQGLLRREPFGRGGQAVARAVERLGW
                     VQIDTISVVERAHHHVLRSRVAGYQPRWLDDLQHRERAVFEYWSHAAAYLPMRAYRFY
                     LPMMRWSRRGDAADPKMAREVLRRVRDEGPLQSRDFEAAGHRSDGWWDWKPAKRTLEQ
                     LFHRGELMVTRREGFQKVYDLPERVLPESVDTSFPDASEWGRFVVGMMIDALGIASDK
                     EIAYQRIAAPRLYSRPILPLVQQAAADMVEAGELEQVTVEGGGSYLTRHLDWLPVRLG
                     RKRVTILSPFDNAVIMRERARELFGFDYAIECYLPAGKRRYGYFSLPILFGDELVGRM
                     DSKADRRTGTLTVNLLHLEPGADPAAIRDPLMQALNGFARDNGCTRVIMGATEPASLK
                     AELARVLG"
BASE COUNT         2449 a         4857 c         5027 g         2523 t
ORIGIN      
        1 gggagccgtt gcccaggtgg ccggcggggt gcccgccatg tgcgacggca tcacccaggg
       61 ccgtccgggc atggagctgt cgctcttcag ccgcgacgtc atcgccatgt ccacggcgct
      121 ggcgctctct cacgacatgt tcgatgcggt ggtctgtctc ggggtctgcg acaagatcgt
      181 tcccggtctg ctcatgggcg cgttggcctt cggccacctg cccgcggtgt tcgtccccgc
      241 cggccccatg ccgtcgggcc tgccgaacaa agagaaggct cgcgttcgag aagcctatgc
      301 tgccggggag gcgggcaggg tcgagctcat cgcggccgag tctgccgcct atcacagccc
      361 cggcacctgc accttctacg gtacggccaa cagcaatcag atgctcctgg agttcatggg
      421 cctgcagctt ccagggggat ctttcgtcaa cccgggctcg gaactgcgcg atctgctcac
      481 tgctgctgcg gtccgacagg tgctggagct cacccgtctg ggcaaccgcc atatgcccat
      541 cggccgcatc gtggacgagt cttcggtggt caacgcggtg gtgggcctgc tggcctccgg
      601 aggctccacc aatcacacca tgcaccttgt ggccgttgcc gccgccgccg gcatccggct
      661 ggactggtcc gacttcgccg atctgtcccg cgtcgtgccg ctgatggcgc gcgtctatcc
      721 gaacggggag gccgacgtca atcacttcca cgacgccggc gggctcggct tcatgatcgg
      781 cgagctgctc gatcacggcc tgctgcacga cgaggtggaa accatcctgg gccgcggcct
      841 cagccggttc gtcgtatgcc ccgagcgggc ggacggcgcc ttgaggtggc ggcccgcgaa
      901 aaaatcgtcc gccgatcccg gcgtgctgcg cggggtcgcg gagccgttcg acgccgaagg
      961 ggggctccgg gtggttcagg gcgatgtggg gcgcgcggtg gtcaaggtat ccgcggtccg
     1021 ggcggagcgt cggcgcatct cggcccccgc ccgggtgttc gtctcccagg acgcctttgc
     1081 cgaggcattc gaggccgaag agctcgaggg cgacatggtc gccgtgctgc cctggcaggg
     1141 tccgcgcgcc aacggcatgc cggagctgca tcggctctcc ccctacttag gcgtgctgga
     1201 gcgtcgggga taccgggtcg ctctgctgac cgacggccgc atgtctggcg cctccggcgc
     1261 cgtgctcgca gccattcagg tgacgccgga ggctgccagc ggggggctga tcggtcggat
     1321 tcgcgacggc gatctgatcc acatcgacgc cgatgcgggc gtcgtgcgcg tcgaggcgga
     1381 tctggagggc cgaacggggg cggctgcaga agcgcccgac ggaacccacg gcaccggtcg
     1441 tgagctgttc tccgcctttc gcgcccaggt cggcccggcc gagtccggcg ccagcatctt
     1501 cgggcccctt tgagatggac gcttgcgatc tgctcgatgg cgtcgggatc gtcgcggtgg
     1561 tggcgctgga ggacgccgat gccgccgtgc cgctggcgcg agttctggcc ggttccgggg
     1621 tcagctgcgt tgaggtcacc ctgcgcacgg gctgcgctgt ggacgccatc gggcggatcg
     1681 cccgggaagt tcccgaggtg ctggtcgggg cgggcagcat ccgccgcccg gagcagctcc
     1741 tcgaggtgcg cgatgccggc gcccgctttg ccgtgagccc gggctcgagc gacgggcttc
     1801 tggcggcttc gcgccggttg aaaatgccct tcgtgcccgg cgccgtcacg gcgacggaga
     1861 tgatcgggct tttggagcac ggttactttc tgcagaaatt cttccccgca gaggcgagcg
     1921 gcggcgccag agcgcttcgc tcgctggccg cgccgctgcc ggaggtgcgc ttctttccca
     1981 ccggcgggat aacccaggaa ctggcccccg ggtatctgca gatgggctcg gtggcctgca
     2041 tcggtggatc ctggttcgtt cctgccgata gcctgcgggc cggcgacttc tctgcgattg
     2101 gccgcctggc cggggccgcg gctgaattga caacgctgtc atagagcggg cctaatcggt
     2161 cggaagccca caggtcgcac ctaccgatga gttccgccga gcactacgcc accgcaccgg
     2221 aagaccggat cagctttgcg cacaaggtcg tctacgggtt cggggccttc gtcaacaacc
     2281 tgctggcagc cgccattggc ggcatggcca tcgtgctgaa cctgggtctc ggcatgaacc
     2341 cggctctggt ggggctgctc ggagcgttgc cgcgactcac cgatgcgctc acagaccccc
     2401 tgatgggata catctcggac cacacaaaca cgcggtgggg gcgccggcgg ccttacatct
     2461 tcgcaggcgc catcgccgcc gggttgtgct tcgcccttct gtggcagttg ccgccaggtc
     2521 gcagcgaaag cttctacttc tactacttcc tcttcggttc gctgatcttc tacctggcct
     2581 acacggtttt cgccacgccc tgggtcgcgc tgggatacga gctgacgccc gactatcacg
     2641 agcgcacccg gctcatgggg gttcagaact tcatcggcca gctggcctat ctgatctctc
     2701 cctggttcct gtggatgatg cagtacgagc cggtcttcga tgacatggtg gagggcgccg
     2761 cggggttgtc ggtagccatc gccgtggtgg tgatgtgcgt gggcgttctg ccggcgttgc
     2821 tgcttcgcga gcggtttcgg gacgtcgctc atgccgagat cgcggaaaag cagctggccc
     2881 gtggggaacc ccggcatccc ttgctgacca acgtgaagtc cttctttgcg ggcttcgccg
     2941 ccacgctcaa gttcaagccg tttgtcaggc tgtgcgccgc cacgtttctc gtcttcaacg
     3001 gcttcatgct ggtctcctcc ttccagttct acgtgatcat ctactacgtc ttttccggtg
     3061 atcaggtgac cggcgcggag tacgcgggct ggtccggcac gctgtcggcc gtctccacgt
     3121 tcgtcgtcat cggcatcgtc acctggcttg ccaccaagat cggcaagcgg cgggctttct
     3181 ttgcagccac ggggatctcc attgtggggt acgggctgaa gtggttctgc tacgacccgg
     3241 cggtgccgtg gctgctgctc gtgccggcgc cgctgatcgc gttcggcctg ggcggtctgt
     3301 tcaccctgat gggctccatg atcgccgacg tctgcgacct ggacgagctc gcaagccatg
     3361 aacggcgcga aggcatgttc gggtcgattt tctggtgggt cgtgaagctc ggcatggcgc
     3421 tggcgctcgc cggcggcggg tttctcttga acgccaccgg cttcgacgtg gcgctcggcg
     3481 gcgctcagcc ggccagcgcg atcctgatga tgcgcgcgtt cgacgtgatc gtacccatgc
     3541 tgagctccgc ggcggctatc tggattatcg ccggcttccc gattacggaa gaacgtgccc
     3601 atcaggtacg agcgcgcctc gaggaacgcc ggggtgccct gaacggcgat ccagggacgg
     3661 ctgcggccgt atagaatgag cggcgtttcg aaagtgtgct gatgggtttt taccgctgag
     3721 cctggaaacg gagtacgcat gagatcgcag aagagcagcc agaaggtcac gatcgtcgat
     3781 gtggccaagc gggcgggcgt gtccatcaag accgtgtccc gggtgctcaa cagcgagccc
     3841 aacgtgcgtg cggacacccg ggaaaaggtg ctcaaggccg ccaaggccct gcattaccag
     3901 ccgaacccct cggcgcgcgg gcttgccggc aaccgttcgt tctcgatcgg tttgctttac
     3961 gaaaatcccc aggaattcgg ttacgtcaag gacgctctga acggcgtcat cggtacctgc
     4021 gaggcggagg gttactcgct cctcatgcgg ccttgcggcg aggaggtgga ttcggaggag
     4081 gttcgcaagt tcgtggcgca gacgcgcgtt gacggcgccg tgttgacggc acccgtgtgc
     4141 gacgagccca aggtcaccga tctgctgaag gagctcggcg tgccgtttgc tcagctcgcc
     4201 ccgaaagcgc cccgatccga gtggatctcg gtgcagtgca acgacgagga agccagctac
     4261 gccatgaccg attacctgat ttctctcggt caccgccgcg tcggtttcat caaggggcat
     4321 cccgatcacg gcgcgacgga gaagcggctg gcgggttaca ggcgctgcct cgcggatcac
     4381 gacatgccct tcgacaaaac cctggtaatg cagggctact tcgatttcga gtccggtcag
     4441 cgctgcgccc ggaagctcct agagctgccg aacccgccaa cggccatctt tgccagcaac
     4501 gacgacatgg cggcgggcgt cattttcacc gcgcgtgagc gcggcatcgg cgttcccgac
     4561 gagctctccg tggccggatt cgacgactct ccggcggcgt cgcacacctg gccgccgctg
     4621 acgaccgtca agcagcccat cgtggagatg gctgcacgag cgacccagcg gctcattggc
     4681 cagctgcggg gtgtgggtga agagggcgac gaggaaatct tcacctgcga gctcatcgtg
     4741 cgacgctcca ccgccaagcg cggcgactga agcgggctga cggcgggccg agacgcctgg
     4801 ccgagatccg ccgcgggccg tgtgccggcc atctgccctg caaacgtttg acaagctcgc
     4861 cgggaatcgg gttaacttcg ggtcaaaatg acagcgttgt catattcgcg gccagtgggg
     4921 tcgcggcggc gcgtcgcgta tctctggcct gatgtttttg aggatcacga tgaccagcgg
     4981 taaacccgcc gattcgaatt tctggccgca ctgcgccagc ccggtggggc aggatgccgc
     5041 aacggagctt cgggtcgccg acgtgctgtc ccggatgacc ctggaacaga aggtcggcca
     5101 gatgatccag gcggagattg ccgccgtcac cccggcccag gcggccgagc atcatcttgg
     5161 gtccgttctc aacggaggcg gttcgtttcc gaacggtgac aagcacgcat cggtggcgga
     5221 ctgggtggcg ctggccgaga gttatcgcgc ggcctccagc gatgcgggtc ctggccggct
     5281 cgggatcccg ttgatctggg gcaccgatgc ggttcacggt cacaacaacg tctatcgcgc
     5341 gaccatattt cctcacaaca tcggcctggg agccgccggg gaccccgacc tgatcgaaga
     5401 gatcggcgcg gccacggcca gagagattgc cgccaccggc atcggatgga cgttcgcacc
     5461 gaccctggcg gtggttcgcg acgaccgctg gggccgcacc tacgaaagct actccgaaga
     5521 tccccagatc atccgcgcct atgccgggcg gatggtcacc ggcctgcagg gtccggccgg
     5581 taccgacgcg ttcctcgggg ccgggcgcgt ggtcgccacg gcgaagcact ttctcggcga
     5641 gggcggcacc gaaggaggcg tggaccaggg cgagaacaac gccgacgaga cgaccctgcg
     5701 cgacgttcac ggtcagggtt acgtcacggc cctgggctcc ggcgcccaga cggtcatggc
     5761 cacgttcaac agctggcggg gcgtaaaggt acacggtcac cgttacctgc tcaccgacgt
     5821 gctcaaaggc cagatgggct tcgacgggtt cgtggtcagc gactggaacg gcttcaagca
     5881 ggtcgccgat tgccgccagg aagcctgcca gctgtcgatc aacgccggcg tcgacatgct
     5941 gatgatcccc gacgactggc aggaggtcat cggcgatctg gtggctctgg tggaggacgg
     6001 ccaaattccc atgtcccgca tcgacgatgc ggtcacccgg atcttgcgcg tcaaagcccg
     6061 cgctggcctg ctcgaccccg cagagtcgtc cgaccggcca cataccgatc cggccgtggt
     6121 tggctgcgcc gcacaccgcg acgtcgctcg acgggccgtt cgccagtccc tggtgatgct
     6181 caagaatcgc ggcggcatgc tacctctcga cccttccctg cacgttctcg tagcgggcga
     6241 cggggcggac gacatcggca agcagtcggg cggctggacg ctgacctggc agggcaccga
     6301 gaacgagaac ggcgatttcc ccggggcgag ctcgatatac gacggcatct ccgccgcggt
     6361 taccaacgcc ggcggccggg ccacgctgtc acctgacggc agctggtccg agcggcccga
     6421 tgtggcggtg gtggtgttcg gagaggaccc gtacgccgag gggcagggag accgcgccca
     6481 tctgagcttc tccgccctgt ttcccgaagg gcttgcgctg ctcaagaaac tgaagggcga
     6541 cggcatcccg gtggtgagcg tctttctcac gggccggcct ctctgggtca accccgagct
     6601 gaacgcttcc gacgccttcg tggtggcctg gcttccgggc tcggagggcg ccggcgtgag
     6661 cgacgtgctc tttcgaaccg tgggtggcga ggtcgctcac gactttaccg gacggctggc
     6721 cttttcctgg ccgcgctcct cgctgcaggc cacggtgaat cgccacgatg cggattacga
     6781 cccgctgttc ccctacggct tcgggctcgg tgccgcagac gtcgataccc tcggtgacga
     6841 gctcatcgag gaagacgagg cgctcggcct tgcggccgac gagagcatcg gcatcctcac
     6901 cggccgcgtg gtgccgccgt tcgagttctt cattggcgac agccaggcct ggaaagtgcc
     6961 ggttcgcggc cgctccagcc ggtctcccac gggcgcggtc tcggtgacca acgtcgacca
     7021 ctacagccag gaggacgcgc gccgcatcag ctgggacggg agcggaggcg ctcaggtcta
     7081 cttccagcgc agcgcgggcc aggacctctc cgaccacgct tcccgcggcg cccgactgcg
     7141 ctgcaagctg cgggtggacg tgcggcccca gcgcagggtc gagctcagga tggactccgg
     7201 ttatcctcag acggcgagca tcgacatcac ggccatgctc aacgagctgc ccctggggac
     7261 ctgggaagac gtcgctttcg agatgagcgc gtttgccggc gggacggaca acctatgcgc
     7321 ggtggatacc ccgtggttga tctggaccga cggcgtgctg ggtctttcgg tgtcgaacct
     7381 tcggatcgat ctgggagacg gatgacttca attctcgggc gctttccagc cgtgctcgtt
     7441 ttgctgtccc tggccgatgc cgggctcgtg ccggaagcgg gcgcgatgcc agatgagccg
     7501 gtcaaagtcg aggtggtgcg aacggcgagg ggctacgcgc tcgagcgcgg tggccggcct
     7561 taccggatcc agggcgtcgg cggtctggag cgccttgcgc tgctcgccga gtccggcggc
     7621 aactccatcc ggacctggtc ggttggaact cccgagcagt ccgcgcggga gctgctcgac
     7681 gaagcgcacc gtctgggcct cacggtcgcg ctgtgcctgg acattgccag ggaacgtcac
     7741 ggcttcgact acgacgatga tgcggcggtg gccgcgcagt tcgagcgagc gagagcggac
     7801 gtgctggcgc accgcaatca tccggccctg ctggcctgga tcattggcaa cgagctgaac
     7861 cacgacttca agaacccccg ggtctacgac gcggtgaacg agatttcccg gatgatccac
     7921 gagatcgacg ggaatcatcc caccaccacg accatcgccg gcttcagcgc ggacatggct
     7981 gaggtcatct ccgcccgcgc gccggacatc gacttcctca gcatccagtc ctacgcaggc
     8041 gtcctcgacc tgcaacgacg ggttcgcaag gccggatacg aggggccgta cgtgctcact
     8101 gagtggggac cgcccggaca ctgggaagtc gcgaccacca gctggggcgc gccgatcgag
     8161 ccggacagct ctgccaaggc caaggcgctg cgccgggcgc acgccacggc gatcgagccg
     8221 tatccggacc agctcatcgg caactacgtc tttctgtggg gccagaagca ggagcggacg
     8281 ccaacgtggt acgggctgtt caccgaaacc ggcgagaaaa ccgagtcggt cgacgtcatg
     8341 cactacctct ggacgggcac ctggcccggc aatcgcagcc ccgcggtcag atcggttcgc
     8401 ctcgacggtc gcagggcggg tcgggacatc gtcctcgttc cggggcagac gtatcccatt
     8461 ctcgcccggg tgcgcgatcc ggaaggggac gccctgcgct atcgctggag cgtcaagtca
     8521 gagagccggg ccaggcaggt cggaggagac ctcgaggggg cgatcgccga cgtggacggg
     8581 ctgatcgacg aaccgtcgtc gagccgcgcg cagctgacgg cccccgaaga gcccggggcg
     8641 tatcgcgtct tcgtctacgc atacgacggc aatggcggcg cggcgcacgc caacgtcccc
     8701 ttcctcgtcc gttctccggc tggagccgac tgaacgcacc acccctttcg gggcctgccg
     8761 tggttcccgc gcccgtgctc ctgatatagt cacgacagac acgaacctgc atagggaaag
     8821 ggaccaatga tcgatttcga gataccggcc gagaccaaag ccatccgcgc caaggcgcgc
     8881 gagttcgtgc agaaggaatg cgtggcggcg gaggcgcaat gcacggcgga taatttcgac
     8941 caggtgcttt cggagctcag aggcaaggcc cgcgcccagg gcctgtggtg tccgttcatc
     9001 cccgaggagc acgggggcat ggggctgaag ccgctggcca acgccctggt tcagatggag
     9061 ctcggcgaga gctttcttgg cgcgctcgcg ctcaacaccc agggtccgga tgacgccacc
     9121 atgctcacgc tgctcgagca cggcaccgac caccagaaag aaaagttctt gaagcccctg
     9181 ctggacggcc agaagcgcat ctgctactcg atgacggaga aggcggcggg tgcggacgcc
     9241 acgggcatgc agaccgcggc cgttctggag ggcgacaact gggtgctcaa cggcgagaag
     9301 tggttcagct cgtcggccga cgtcgccgac atcgccgtgg tgatggccaa gacggacccg
     9361 gacgcgccgc gccacgagcg ctacagcacg ttcatcgtcg agctgcccaa cccgggctat
     9421 cagatcctgc gcaacatcga caccatgcag ccccacactg agctgggcct caagctcggc
     9481 cactctcact ccgaggtgaa gatcgaaaac ctcgtggtac cgcgcgagaa cctcctcggc
     9541 ggccggggca agggctttgc catgggccag caccggcttg cctacggccg gctgcgtcac
     9601 ggcatgcaca acgtggccat ggcgcagcgg gcgctggatc tggccgccgg gcacgtggtc
     9661 aatcgggaga ccttcggcaa acggctcgcc gataggcagg gcgttcgctg gatgctggcc
     9721 gactgcgcgg cggagatcta caaggcccgg ctgatgcttc tgcacatcgc ctacaaggcc
     9781 gagcgaggca tggacttgag gcaggagaac gggatcgcca aggtgttcct cgcgcacatg
     9841 gtgcatcagg tggtggacac cgcgctgcag ctgcacgggg cgctgggcta cagccacgac
     9901 acgccgctgg cgagctggta cacgagcatc cgcagccagc ggctcgtgga cggcccggac
     9961 gaggtgcacc gctggcgcac gggcgccaac gtgatcaagg cttacgagga gcacggtacc
    10021 acggcgcccg cctgcggcgg cgagctgctg tgagcgaggc ccggacagag cccgccgttc
    10081 gcgtggatcc cgcgaccggg gtcaaacgct ttgccacccg cgcggcaaag gccacggaca
    10141 agatcgcggg caagggctat tcggtgattg ccgacgaggc gttgacgacg ctgccgcccg
    10201 ctccgccggg cgcggtgttc gacgccgagg agcaggcgaa gtaccgggcc ttcaaggagg
    10261 tccgtcgcgg ggccgccgac tacatgacca tggaaggcga gttcgcgagg tacttagagg
    10321 acgtctattc cgcggatccg gtgccccggg aagcgttgac cgacgagtgc gaggtgctcg
    10381 tggtcggggc gggtttcgcc ggtctgctcc tttggcagaa gctttccgca gccggattca
    10441 ccgacgtgcg cttctgcgag aagggcggcg acgtgggcgg cacctggtac tggaaccgct
    10501 acccgggcat cgcctgcgac gtcgagtcct acagctacct cccgctgctg gaggagatgg
    10561 gttacatccc cagcatgaag ttcgcctcgg gtttcgagat cctcgagtac tgccagcgga
    10621 tggcggaaaa gttcggcttc tacgatcgct gcctgtttca caccaccgtg gatcgcacgg
    10681 agtgggatga ggcggccggt cgctggacgg tttacacgaa tcgcggcgac gccatgcgcg
    10741 cgcgcttcgt ggtgctggcc aacggcatcc tcaccacgcc ccagctcgcg cgtatcgacg
    10801 gtatggagac gtttaagggc gagtccttcc acacatcacg ctggaactat cacgtggatc
    10861 tggccggcaa acgggtgggc attatcggca ccggcgccac ggccgttcag gtgatccccg
    10921 agattgccaa gatcgtgaag gagctctacg tctttcagcg gacgccgtcg tccatcgacg
    10981 tgcgcgatca gcgggagacc acgccggaag agtacgagac ctggtcgcac gaacccggct
    11041 gggcccgggc gcggcgcgcg cgattctcga agatctcctc cgggcggacg gccatcaagg
    11101 ccaacgacga ctacctgtcg ggaaaggtgg ccgacttcaa ggagcgcaag cagcacgagc
    11161 gcaggctctc cgccgaggag atgatcaaca agcagctcga gaccaacttc cgcatcatgg
    11221 agcagatccg cgcccgcgtg gacgccatcg tcgaggaccc caggacggcc gaggcgctga
    11281 aaccctacta cccgtacggg tgcaagcggc cgacgttcca tgacgagtac ctgcccacct
    11341 tcaacctgcc tcacgtcacc ctggtggaca ccgcgccgct gggggtcacc aggatcaacg
    11401 agcgtggcgt cgtgcacgac ggccgggagt accccctgga cgtgctgatc tacgccaccg
    11461 gcttccagtg gatggcgacg tccacgttca acatgatccg tggacggggt gggcgcacgc
    11521 tcaacgagaa gtggcaggag gaggggaccc gcacctttct cgggatacac agtcatggct
    11581 ttccgaacct cttcatcgtc tcgggtcccc agggcggcgg cggcagcttc aacttcaccg
    11641 atgccatcga cacccacgcc gactacatct gctggatgct ggagaccatg cgcgaccggg
    11701 gcgcccgtac ggtagacgtg cgcaaggaac cggaagacga gtatgccgag cactgtcggg
    11761 cggcggacgt catgacggcc cccctgcgcg actgcatttc ctactacaac ggtcacgggg
    11821 acgcggagcc tggcagcctt gcctattacg gcggcggccg ctggcacaag tatcgcgttc
    11881 gcgctcagga gagtctggaa ccctacgatt tcgacgatat tccggagtag tggaaacccc
    11941 tggggatcgg cgagtactgc aacggacgcg gtttgatccc cccgcggctg tcgatatagt
    12001 tcagggatag ccggcctgga gaggggatga tgaccgatct cgttcgcaaa ggatggaccg
    12061 ttctggccct ttgggccttg accatgattc tcggctactt cggtctcggc ggctttctcg
    12121 ccgagacggt tgaaggtatc ggtggggtac ccgcttggcc cacggcgcta ttcgtggtcc
    12181 tcttggccgt gagtctctgg gccagcgtgg tgtgggcgcg cgggctgaaa gcgaagcccg
    12241 aagcgggggc cgcgctggaa gaaaggccac tcgccagcgg gcgacgtcgt tttctcacgg
    12301 gtaccgcggc ggtgaccggc ggcatgctcg gggccgcggg ggcgaccatc ggcagggtgg
    12361 caccctgggc gacggtgacg cttcctgccc tgcgccagcc cgtgaccgtc acggatccga
    12421 acccgaagga cgactggctg ggcgccaggg tgcgcagcta ccggcctttg ggagacaccg
    12481 gattgatggt ctccgacatt gccaccggct ccacgcgcat tcaccacaac tcggatccgg
    12541 tcgggtacct gaagagcctt ctggaccgcg gggtcaacta catcgacgcc tcacccgact
    12601 acgccccgga gagcgagggc atcatcgccg aggccattgt cgggcggccc cgggaccagc
    12661 tggtcatcgc ctccaagttc tgtactcgcg acggccatct ccggcagggc tcgagcgtcg
    12721 cagcttacat gaccgccatc gacgggacgc tcagccggat gggcacggac tacatcgacg
    12781 tcgcccacat ccattcctgc gacagcaccg aaaggctcat ggacgagaac gtccacgagg
    12841 ctttcgatcg cctcaaggat gccggcaagg tgcgccatct gggagtgtct acccacacgc
    12901 ccaacttaga ggaggtcgcc gacaccgcca tcgacagcgg ccgtttcaag gtgatgatgc
    12961 tggcctacca ccacggcgcc tggccgaagc agatggacat catcgatcgc gcggccgcgg
    13021 cgggcatggg catcgtcgcc atgaagacgc tcaagggcgc caagcaccgc ggccttgcgg
    13081 aattcaggcc cgaagcggac tcctacgccc aggcggcctt caagtgggtg ctggccaacc
    13141 cgtccgtttc caacctggtg gtttcgtttt acgacaacca gcatccggac gagtacctgt
    13201 tcgcctcggg caagtccctc gagcggagcg acatcgcggt gctcggcaaa tacgaccagc
    13261 tcattgcggg cacccactgc ttccagcatt gcggcgattg cctcggggcg tgtccgagtg
    13321 ggctcgccat caacgacatc ctgcgtcatc gcatgtactt cgaggattac ggggacgaaa
    13381 agcaggccat gcagctttac gccaagctgg accggcaggc ggacgtctgc atcgactgct
    13441 cagcaccctg cctcggcagc tgtccccagg gggtcgagat tcaggagcgc atgatcggcg
    13501 cccacgagaa gctgcgctgg gcctgagacc ttgtagccga ttgggccttc gccccggtca
    13561 gccgaggacg cgagcaagct cggctttcag cgatgcgggt tcggttgcgc ccatgatgac
    13621 ccgggtgcag ccgttgtccc gggcaaaccc gttcagtgcc tgcatcaagg ggtctcggat
    13681 cgcagcggga tccgcgccgg gttcgagatg gagcaggttc accgtcaggg tgcccgtgcg
    13741 ccgatccgcc ttgctgtcca tccgcccgac gagctcgtcg ccaaacagga tcggcaggct
    13801 gaaatagcca tagcgccgct tgcccgcggg caggtagcac tcgatggcgt agtcgaagcc
    13861 gaacagttcc cgggcacgct cccgcatgat cacggcgttg tcgaacggcg agaggatcgt
    13921 cacgcgtttg cggcccagcc gcaccggcag ccagtccagg tgacgcgtca ggtacgagcc
    13981 gccgccctcg accgtgacct gctccagctc ccccgcctcc accatgtcgg cggccgcctg
    14041 ctgcactaac ggcaggatcg ggcgcgagta gagcctgggc gcggcaatgc gctgataggc
    14101 gatctccttg tcgctggcga tgcccagggc atcgatcatc atgcccacca cgaagcggcc
    14161 ccactcggaa gcatcgggaa acgacgtgtc cacgctctcg ggcagcaccc gctcgggcag
    14221 atcgtagacc ttctgaaacc cctcgcgccg ggtcaccatc agctcgcccc ggtgaaagag
    14281 ctgctcgaga gtgcgcttcg ccggcttcca gtcccaccag ccgtcgctgc gatgaccggc
    14341 cgcctcgaag tcccgggact gcaggggacc ctcgtcgcgc acgcgtcgca gcacttcccg
    14401 ggccatcttc ggatccgcgg cgtccccgcg ccgcgaccag cgcatcatgg gcaggtagaa
    14461 gcggtaggcg cgcatgggca gataggcggc cgcgtgggac cagtactcga acaccgcccg
    14521 ttctctgtgc tgcaggtcgt cgagccacct gggttgatac ccggccacgc ggctgcgcag
    14581 cacgtggtga tgggcacgct ccaccaccga gatggtgtcg atctgcaccc agcccaggcg
    14641 ctcgacggcg cgggccacgg cctgaccccc gcggccgaac ggctcgcgcc gcagcagtcc
    14701 ctgccggtgc aggaacaggc ggcgggcgtc gcgggtggaa atgtcggtga cggtcggcat
    14761 gtgggctcag gcgcctgcgg gtcttgcgtc gacgccgcga gcatagcggc tcgctcgacc
    14821 tgatggccat ggcggccggg tggatcgagg cgcatg
//
LOCUS       DBNHKV010000091         7602 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1501880, whole genome shotgun sequence.
ACCESSION   DBNHKV010000091 DBNHKV010000000
VERSION     DBNHKV010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7602)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7602
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1501880"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="AC16PP_02955"
     CDS             complement(<1..500)
                     /locus_tag="AC16PP_02955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6480612.1"
                     /translation="MSKENELAEIRNAVRQLCAQYGEDYWLELDRINGYPTEFVKELT
                     TSGFLTVLIPEEYGGAGLGVLEAAAVMEEVCRSGAHAGACHAQMYVMGSVLRHGNDEQ
                     KRKYLPKIASGELRLQSFGVTEPTTGTDTTSLKTTARLDGDSYVVNGQKVWISRIQHS
                     DLMVLLA"
     gene            796..1065
                     /locus_tag="AC16PP_02960"
     CDS             796..1065
                     /locus_tag="AC16PP_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480613.1"
                     /translation="MAVVLAMGCAANAIADEEAVDSLQFEETFGFDISQYQLDDYTEV
                     TGWRLSDRWYFGRQKGVDSGLGFVWQDEADQMSISTNGIRFTRRF"
     gene            1172..2374
                     /locus_tag="AC16PP_02965"
     CDS             1172..2374
                     /locus_tag="AC16PP_02965"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6480614.1"
                     /translation="MPVTIPDPDPNNAWRLETPGSQGWTRTARATDPDRYFVVSADGH
                     VQEPSDLWRTRMDAKYRDRLPGVAMDAKGQKFQKTEGFRPLRLQNIKFEGEDALRNQS
                     GHTPEERLADLAADGVDCEILFPNKGLTMWATPDAAFSQAMCRVFNDWAWEVFGPYND
                     RLAPMACVASADIPGAVEEIQRCARLGFRGVTLPCKPVWGAPDHEALNYNLPDFDPLW
                     ACVQDAGLPVTFHVSTGRDPRTARGNGGAVINYAVHSLAPTMEPIANICASGVIDRFP
                     KLRFGTIEAGIGWVPWTLAALDEAYKKHHMWVRPKLKMLPSDYFRQNGFASFQEDQPG
                     LDLAREHRLIDNFLWANDYPHHEGTWPHSAEAIERTMGHLTDSERAKILGLNAASIFG
                     FEVPRGKA"
     gene            complement(2378..2776)
                     /locus_tag="AC16PP_02970"
     CDS             complement(2378..2776)
                     /locus_tag="AC16PP_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6480615.1"
                     /translation="MHPNEALFANESFYLAFAAGDSDAMEQLWSRQHPLVCIHPGWPA
                     LVGRESVIDSWRQILANPDQPEVEFYGARAVELGSGIAVVCYEVLPGTVLVAMNLFIA
                     ENGEARLVHHQSGPCGDPPPRDAVVRRLDA"
     gene            2895..4748
                     /locus_tag="AC16PP_02975"
     CDS             2895..4748
                     /locus_tag="AC16PP_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6480616.1"
                     /translation="MSTLHKPTCLIALFLSVTLMTPPGWTAAREIGPNHWAGVSRVVA
                     FGDVHGAHDQMVTALESAGVVDAELNWAAGDTHLVSLGDLLDRGAHSRQTMDLLMRLQ
                     RQAKGAGGRVHVVLGNHELMNLTGDMRYVAADEFAAFADDEDPSVREAAREAFVAAAD
                     PASGKDPSVEFDERYPAGYFGHRAGFAAAGSYGDWLLQLPLMVVVNETAFVHGGLPAL
                     IADQTLEEINRQYQRDLGEIMALGGRLADAGWVSAERDLLLQDDAVLARLEAAAEGDD
                     GDLSRAGTRFIELVRGPLFGSRGPNWYRGTAMCHASLEETVLKAALKALGAKRVVVGH
                     TPTSNRRVQQRMDGRAILADTGMLRSYYSGQPAPVVLTGDDIEILYPLSETRADAPDV
                     IDPLARYGLDQPRLEAVMSNAAIAEVSTAGTGQRFTTVTLLDEGKRYEFAFERGQGSD
                     NDRKVGAYRLSRLLELNVVPVTVSRTVEGAKGVLIALPEKRVSEIERANQGLSRSNWC
                     SFGNDYQLMYAFDALIRNEQRTTDTMIYDQEDWQLTLIGHEASFGNGTDLPAYLAAVE
                     KILPAAMAARMAAMNEDSLTAALGDQLSKRQIRALLKRRDRMIKDWQLQAL"
     gene            4748..5392
                     /locus_tag="AC16PP_02980"
     CDS             4748..5392
                     /locus_tag="AC16PP_02980"
                     /inference="COORDINATES: protein motif:HMM:NF027640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HIR6480617.1"
                     /translation="MRKSTRASAPVFIITVYYVVLLGGFMAASSYLPGLRVYLPVDGI
                     SELIERGGASFEPVEIIRTAIVEAPHMARLALGMIGATLLMLPISWVYFITTPKSAVD
                     RSFAQTMIMLPIIVAGIAMIVQNSIALAFSLAGVVAAVRFRFTLTQPSYTLYIFAAIT
                     IGLGGGIAAIEISTVVSIAFVYATMLMWMSDYGADMNTSFFSFLTGRDRREEDP"
     gene            5376..6236
                     /locus_tag="AC16PP_02985"
     CDS             5376..6236
                     /locus_tag="AC16PP_02985"
                     /inference="COORDINATES: protein motif:HMM:NF018659.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HIR6480618.1"
                     /translation="MKKILRAFASLAACIAFSFLPGCGSHDETFAFGGYRFATDTFQQ
                     WKLPKSLREISGLAATADGRLFTHGDERAEIYQIDYRDGKLVKSFRLGISSDSRPVKG
                     DFEGIAVRDSELYLITSNGILYRSQIGGDGSTMPYQTFNSGAGDLCEVEGLAYRPQTD
                     MLLIACKEVRKVEFEDKVVVLQWSPQTRRLERDMSILINQSSTGGRFSASGISVSPRN
                     GNLLLVSARQRRLLEVTPEAHVVAVLSLPMERYHRQTEGIEVTGSGTLVLADEGGNKR
                     GRLGTYRGRN"
     gene            6211..7176
                     /locus_tag="AC16PP_02990"
     CDS             6211..7176
                     /locus_tag="AC16PP_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480619.1"
                     /translation="MELIEAEIKPPKTIIMVHGRGWKPARQALESLWVESLRTGLQRD
                     AESQSTSVALDMAKIQMVYYGDLTNAIRAGRGKAYDEALDLADLTNALDSLKALHTAK
                     KFRRARYEALPGRSSLEEFLADIGAPILSTLRLTDKVIGRRMPEVVAYWDDRDGFSSQ
                     ICARLLNEIRKAKDEDSDILVISHCLGTIAAYDAFWELSRGQDADPSAKIDTWITMGS
                     PLGDEFVKRQLAGASEAGERRYPCNILNWYNVAAEDDFTCHDETVANDFSQMLDYHLV
                     SRIRDFRIYNLALRYGRSNPHNALGYLVHPRVSSLVGEWLLADLS"
     gene            complement(7173..>7602)
                     /locus_tag="AC16PP_02995"
     CDS             complement(7173..>7602)
                     /locus_tag="AC16PP_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480620.1"
                     /translation="ELYQYLISNTDFSFLAGPAGEACCHNSVLFSNAAGEVIPIPYDF
                     DVSGLVDAPYAVPPDGLGQRNVRDRKYRGFCRGTEYLDAAVETIQSKRQAIYALVDEV
                     EGFSDRTRRDLSRFIDEFYGDLDDPESFEENIAEACRSLR"
BASE COUNT         1504 a         2387 c         2278 g         1433 t
ORIGIN      
        1 gccagaagca ccatcagatc tgagtgctga atccggctga tccagacttt ctgcccgttg
       61 accacgtagg aatcgccgtc aaggcgtgcc gtggttttca ggctggtggt atccgtaccc
      121 gtggtcggct cggtcactcc gaagctctgc aggcgcagct ccccggaagc gattttgggc
      181 aggtatttcc gtttctgctc gtcgtttccg tgacgcagta ccgaacccat cacgtacatc
      241 tgagcgtggc acgcgcctgc gtgggcgccc gatcggcaga cctcttccat aaccgccgcg
      301 gcctcgagca cgccgagccc ggctcccccg tactcctccg gaatcagcac ggtgaggaac
      361 cccgacgtgg tcagctcttt gacgaactcg gtgggatagc cgttgattcg atcgagttcc
      421 agccagtaat cctcgccata ctgcgcacac agctgcctga cggcattgcg gatctccgcg
      481 agctcgtttt cctttgacat cgcacttccc cggaaccagg cggaacgata ctgcatgcga
      541 caccgcgatt cacgttccag cgccaactcg gtcccgcgct cccgcaaaac gatgaggcca
      601 ccgggcgtcg cgcagccagt taacaaactt ttacaacctc catttttata aattaattca
      661 ttattttcat gaggttacga attttttctc aatcattttc tgctcatcgc aaatccatgg
      721 tatttgggac gtttccgcag ccttctgtga cacatttgca ccatggctaa aacgtcaaaa
      781 catctgatcg gatcaatggc cgtcgttctg gcaatgggtt gcgccgcaaa tgccatcgcc
      841 gacgaagaag ccgttgactc cctgcagttc gaggaaacgt tcgggttcga catcagccag
      901 tatcagctcg acgattacac cgaggtaacc gggtggagac tgtccgacag gtggtatttc
      961 ggccgtcaga agggggtcga ctcgggtctg ggtttcgtct ggcaggacga ggctgaccag
     1021 atgagcatat cgaccaacgg catccgcttc acgcgccgtt tctgagtatc cggtccgcct
     1081 ctcagggagg gccttccggt cccccacagc cggtcgtccg cgttacccga tacgggtagt
     1141 attagctcgt aatcccctac ggagcccgtc gatgcccgtc accattcctg accccgatcc
     1201 gaacaacgcc tggcggctcg agactccagg cagccaaggc tggacgcgca ccgcacgtgc
     1261 aaccgacccc gatcgttact tcgtcgtctc cgcagacggc catgtgcagg agccatcgga
     1321 tctgtggcga acgcgcatgg acgcgaagta ccgcgatcgc ctgcccggtg tcgccatgga
     1381 cgctaagggc caaaagttcc agaagacgga gggctttcgg cctctgcggc tgcagaacat
     1441 caagttcgaa ggtgaggacg ctctgcgcaa ccaatccggc catacacccg aagagcgtct
     1501 ggccgatctt gcagcggacg gcgtcgattg cgaaatcctg tttccgaaca aagggctgac
     1561 catgtgggcc acaccggatg ccgccttcag ccaggccatg tgccgggtgt tcaacgattg
     1621 ggcatgggaa gttttcggcc cttacaacga tcggctcgcg cccatggcct gcgtggcgag
     1681 cgccgacata cccggagcag tcgaggagat tcagcgctgc gccaggttgg gctttcgcgg
     1741 cgtgaccctt ccctgcaaac cggtgtgggg cgctccggat cacgaggcgc tcaactacaa
     1801 cctgccggat ttcgatccgc tgtgggcatg cgtgcaggat gcgggtctgc cggtcacctt
     1861 ccacgtttcc accgggcgcg atccgcgcac ggctcgcggc aacggcggcg ccgtcatcaa
     1921 ctacgccgtg cattcgctgg ccccgaccat ggagcccatc gccaacatct gcgcgtccgg
     1981 cgtgatcgat cgtttcccga agctccggtt cggaaccata gaggcgggca tcgggtgggt
     2041 gccctggacc ctcgcggcgc tggacgaagc ctacaaaaaa caccacatgt gggtgaggcc
     2101 gaagctcaag atgctaccca gcgactactt ccggcagaac ggcttcgcca gcttccagga
     2161 ggatcagccc gggctggacc tcgcccgcga gcatcggctg atcgacaatt ttctgtgggc
     2221 aaacgactat ccccaccacg agggcacatg gcctcattcg gccgaggcca tcgagcgaac
     2281 catgggccac ctgacggatt ccgagagagc aaagatcttg ggtctcaacg cggcaagtat
     2341 cttcggcttc gaggtcccgc gggggaaggc ctaggagtca ggcgtccagg cggcgaacga
     2401 ccgcgtcgcg cggcggcggg tctccgcagg gccccgactg atggtggact aggcgcgctt
     2461 cgccgttctc agcgatgaac aggttcatgg cgaccagcac cgtccctggc agaacctcgt
     2521 aacagaccac cgcaatgcca ctgccgagct ccaccgcccg ggcgccgtag aactccacct
     2581 cgggctgatc gggattagcg aggatctgtc gccagctgtc gatgaccgac tcacggccta
     2641 caagcgccgg ccacccggga tgaatgcaga caagcgggtg ctgccgtgac cagagctgct
     2701 ccatggcatc cgagtcgccg gccgcgaaag cgagatagaa cgactcgttg gcaaacagtg
     2761 cttcgttcgg atgcatgatg gcgcgagtat ccacgctctg ggagaatggc tcaaggcgag
     2821 ttcgcccggc gtccgtcgtt gcaaatggag ccggcttttc gtgaaatggg ggcgactcgg
     2881 gtcggacttc tgctttgagc acgctgcaca aacccacttg cctgatcgct ctttttctga
     2941 gcgtgacgct catgacgcca ccgggctgga cggcggccag ggaaatcggt cccaatcact
     3001 gggccggcgt gtctcgcgtc gttgcgttcg gagacgtcca cggcgcgcac gaccagatgg
     3061 tcaccgcatt ggaatccgct ggcgtggtcg acgccgagct caactgggcc gccggcgaca
     3121 cgcatctggt gagcctcggc gatctgctcg atcggggcgc gcattcccgg cagaccatgg
     3181 atctgctgat gagattgcag aggcaggcca agggagcagg cggccgggtg cacgtcgtcc
     3241 tgggcaatca cgagctcatg aatctgaccg gcgacatgcg ctacgtggcc gccgatgagt
     3301 ttgcggcgtt tgcagacgat gaagatccgt cggtgcgcga ggcggcgcgc gaggcgtttg
     3361 tcgccgcggc ggaccccgct tccggcaagg acccatctgt ggagttcgac gagcggtacc
     3421 cggccggata ctttggtcac cgggcgggat ttgccgcggc cggcagctac ggagactggc
     3481 tgctgcagct gccgctgatg gtggtcgtca acgagaccgc attcgtccac ggcgggctcc
     3541 ccgcgctgat cgccgatcag acgctcgaag agatcaaccg gcagtaccaa agggaccttg
     3601 gcgagatcat ggcgttgggc ggccggctgg ccgatgcagg atgggtcagc gccgagcgcg
     3661 atctgctgct gcaggacgat gcggtgctgg cgcggctgga agccgccgcc gagggagacg
     3721 acggcgatct ttcccgagcg ggcacgcgtt tcattgagct cgtaagaggc cccctcttcg
     3781 gatcccgagg accgaactgg tatcgaggca ccgccatgtg ccacgcctcc ctcgaagaga
     3841 cggtgttgaa ggctgcgctg aaagccctgg gagcaaagcg cgtggtcgtg ggtcataccc
     3901 ccacgtcgaa ccgccgcgtt cagcagcgaa tggacggtcg cgcgatcctt gccgataccg
     3961 gcatgctccg ctcttactac agtggccaac ccgccccggt ggtgctgacc ggcgacgata
     4021 tcgagattct ctaccctctg agtgaaaccc gcgccgatgc tcctgacgtc atcgacccgc
     4081 tggcacgcta cgggctggat cagccgcggc tggaggccgt catgagcaat gcagccatcg
     4141 cagaagtcag caccgccggg acgggccagc ggtttaccac cgtcacgctg ctcgacgaag
     4201 gcaaacggta cgagttcgcg ttcgaacgcg gccagggttc agacaacgat cgcaaggtcg
     4261 gcgcttaccg cctgtcccgc ctgctcgagc tcaacgtcgt gcccgtaacg gtcagccgca
     4321 ccgtcgaggg cgccaagggc gtgctcatcg ccctgccgga gaagcgcgtt tcagagatcg
     4381 agcgggcaaa ccagggactg agccgttcga actggtgcag cttcggcaat gactaccagc
     4441 tgatgtacgc tttcgacgcg ctgattcgca acgagcagcg tactaccgat acgatgatct
     4501 acgaccagga agactggcag ctgacgctca tcggccacga ggcgagcttt ggcaacggca
     4561 ccgacctgcc cgcctacctg gcggcggtag aaaagatcct accggcagcc atggccgcgc
     4621 gcatggcagc catgaacgaa gacagcctca cggctgcctt aggcgaccag ctgagcaaac
     4681 ggcagattcg cgcgttgctc aagcggaggg accgaatgat caaagactgg cagctccagg
     4741 ctctgtaatg cgcaagtcga cacgagcatc tgcgcccgtc ttcatcatca cggtttacta
     4801 cgtcgtcctc ctgggcggct tcatggcggc atccagctat ctgcccggtc tgcgggtata
     4861 cctcccggtc gacggcatct cagagctcat cgagcggggc ggcgcctctt tcgagcccgt
     4921 cgagatcatc agaaccgcca tcgtcgaggc accccatatg gcccggctgg ccctgggcat
     4981 gatcggggcc accctgttga tgctgcccat ctcctgggtg tacttcatca ccacgccgaa
     5041 gagcgccgtg gatcgctcgt ttgcgcagac gatgatcatg ctgcccatca tcgttgccgg
     5101 cattgccatg atcgtacaga acagcatcgc gctggcgttc agcctggccg gcgtcgtggc
     5161 tgcggtgcga ttcagattca cgctgacaca gccctcttac acgctctata tatttgccgc
     5221 cataaccatt ggtctcggcg gcggcattgc cgctatcgaa atttcgacgg tggtctccat
     5281 tgccttcgtc tacgccacca tgctcatgtg gatgtcggac tacggcgcgg atatgaatac
     5341 cagcttcttc tcatttctga cgggacgaga ccgtcgtgaa gaagatcctt agggcttttg
     5401 ccagtctggc agcctgtata gccttcagtt tccttcccgg atgtggttcc catgacgaaa
     5461 ccttcgcgtt cggcggttac cgcttcgcca ccgatacctt tcagcagtgg aaactgccga
     5521 agtccctgag ggaaatctcg ggccttgccg ccaccgcgga cgggcggctg tttacgcacg
     5581 gcgacgagag agccgagatt taccagatcg actaccggga cggcaagctg gtgaagtcgt
     5641 tcaggctggg aatctcgtcg gacagcaggc cggtcaaggg cgatttcgaa ggcatcgccg
     5701 tacgcgacag cgagctatac ctgattacca gcaacgggat cctctaccgc tcacagatcg
     5761 gcggcgacgg gtcgacgatg ccataccaga ccttcaattc aggcgcgggg gatctgtgtg
     5821 aggttgaggg cctggcctac cgcccgcaaa cggacatgct gctgatcgcg tgtaaagagg
     5881 ttcgcaaggt ggagttcgag gacaaggtcg tcgtgctgca gtggtctccg cagactcggc
     5941 gcctggagcg cgacatgagc atcctgatca atcaatcgtc cacgggtggc cgattcagcg
     6001 cttccggaat cagcgtttcg ccacgcaacg gtaatctgct actggtctcc gctcggcagc
     6061 gaaggctcct tgaggtgaca cctgaagccc acgtcgtcgc cgtattatcg cttcccatgg
     6121 aacgctatca tcgacaaact gagggcatcg aggtgacggg cagcggaacc ctcgttctag
     6181 ctgacgaggg cggaaataaa aggggccgac ttggaactta tcgaggcaga aattaagccg
     6241 cccaagacca tcatcatggt ccacggccgc ggctggaagc ctgcaaggca agcgctcgag
     6301 tcactctggg tcgaatctct acggaccggc ctgcaacgtg acgcggaaag ccagtcgaca
     6361 agcgttgcgc tggatatggc gaagatccag atggtctatt atggcgacct gaccaacgcc
     6421 atcagggccg ggcgcggaaa agcctatgac gaggccctcg accttgcgga cctgaccaac
     6481 gcgctggact ccctgaaagc gctgcacaca gccaagaagt ttcgacgcgc ccgttacgaa
     6541 gcccttcctg gtcgcagctc gctggaggag tttttggccg acatcggcgc accgattctc
     6601 tccacactgc ggctgaccga caaagtcatc ggccggcgaa tgccggaggt cgtcgcttac
     6661 tgggacgatc gggacggttt cagctcccaa atctgcgcga ggctcttgaa cgagatccga
     6721 aaagccaagg acgaagattc cgacattctc gtcatttccc attgcctcgg cacgatcgcg
     6781 gcgtatgacg ccttctggga actctcgcgc ggccaagatg ccgacccgag cgcaaagatc
     6841 gacacctgga tcaccatggg ctctcccctc ggagatgagt tcgtcaaacg acaactcgcc
     6901 ggcgcctctg aagccggcga gcgccggtac ccctgcaaca ttctcaactg gtacaacgtc
     6961 gctgcagagg acgatttcac ctgccatgac gagaccgttg ccaacgactt cagccagatg
     7021 ctcgactacc acctcgtcag tcgaatacgc gacttccgga tctacaatct ggcgcttcga
     7081 tacgggcgct cgaatccgca caacgcgctc ggctacctcg tccaccccag agtgtctagc
     7141 ctggtcgggg aatggctgct tgccgatctg agctagcgca gactccgaca ggcttccgct
     7201 atgttctctt caaacgactc ggggtcgtcc agatcgccat aaaactcgtc aatgaaccgc
     7261 gacaggtcgc ggcgggtgcg atccgaaaat ccctctacct catcaaccag cgcgtaaatg
     7321 gcctgacgct tggactgaat cgtctccacc gcggcatcga gatactcggt tccgcgacag
     7381 aatcccctgt acttgcgatc tcttacgtta cgctggccga gcccatccgg cggcacagcg
     7441 tacggcgcat cgaccagccc cgacacgtca aaatcataag ggatgggaat gacctctcca
     7501 gcggcattgg agaacagcac cgagttgtgg cagcaggctt cgccggcagg tcctgcaaga
     7561 aaagaaaagt cggtgttgct gatcagatac tggtacaact cc
//
LOCUS       DBNHKV010000092         2553 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_651824, whole genome shotgun sequence.
ACCESSION   DBNHKV010000092 DBNHKV010000000
VERSION     DBNHKV010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_651824"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1678)
                     /gene="xrtE"
                     /locus_tag="AC16PP_03000"
     CDS             complement(5..1678)
                     /gene="xrtE"
                     /locus_tag="AC16PP_03000"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase E/protease, VPEID-CTERM system"
                     /protein_id="HIR6480621.1"
                     /translation="MRLAINQAFSYLLTMQSGIPLSGKAPNLFGLQLALVSALLVSEM
                     FFISYRYDAWTLLQTTDSPWLTWLAYGGYFLKSAIAFVAALLVTIGTRLKAHYDDLVT
                     VTAPRSPWALLLAQLAAYAVLVWLTHLIFADPAEGAPPDAIIVGAWVLATTATVCIWL
                     SLIAPFSYWRRLVRQERTPIAASVLVASVALVIGASSQQLWGPLSQMTFASVEWVLWL
                     IDDEVYVDRERMILGLPYFQSRIAEPCSGYEGIGLVTVFTTLYLAIYRDHFKFPNALL
                     LFPIGIIAIWCANVLRIVALIVIGNGFSPAVAVGGFHSFAGWIAFITVAVAMLVYADR
                     HHFFATKTAPRSGARLGMPGVLLVPFTFLLATTLLTLAFSGDFVWLYPARVIVTGIVL
                     AMLWRRLELGSWRPGLIPVAAGFAVFVLWIAMVPVDEAGSAAFTAALTEAGPLAAAGW
                     MIFRVIGSVITVPIAAELAFRGYLMIRLSGSKVGETDRLRFSWIALIVSSVLFGLMHA
                     DWLAGIMAGLAYGLVRYHRGSLTDAIVAHAMTNLLLTVYVITTGHWSVW"
     gene            1694..2476
                     /locus_tag="AC16PP_03005"
     CDS             1694..2476
                     /locus_tag="AC16PP_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HIR6480622.1"
                     /translation="MRHRLLGLADHRSPFSGCGRAELLRAALSWGRRVRNRRESVLND
                     RLTIDYFSDVLCVWAWIAQRRIDEMNDQWGETVRLGFHCVDVFGDAGAKVAAGWSARG
                     GYEGFAQHVREVAIRYEAPAVHEYLWTKVRPVTSGTAHLVLKATEIAESPLEMTRLSR
                     ALREAFFVDARDVGDLGVCLEVAQGLGLAAAELKRHVDSGAAMAALLADYRKAEERGI
                     RGSPSWVMNEGRQVLYGNVGYRILNANIEELLRHPEQEASWC"
BASE COUNT          528 a          759 c          863 g          403 t
ORIGIN      
        1 ggtctcacca gaccgaccag tgtcccgtcg tgatgacgta gaccgtcaag agtaggttcg
       61 tcattgcgtg cgcgacgatc gcgtccgtca gagaaccgcg gtgatagcgc accagaccgt
      121 aggccagccc cgccataatc ccggccagcc agtcggcgtg catcagcccg aatagaaccg
      181 acgagacgat cagagcgatc cacgagaatc gcaagcgatc ggtctcccca accttgctcc
      241 ccgatagccg aatcatcaga tagccccgga aggcgagctc cgcggcgatg ggcacggtga
      301 ttaccgaacc gatgacccgg aagatcatcc agcctgcggc agccagcggg cccgcttcgg
      361 tgagcgcggc ggtgaacgct gcgctgcccg cttcgtccac gggcaccatg gcgatccaca
      421 gcacgaacac ggcgaaacct gcggcgaccg gaatcagccc gggtcgccag gaaccgagtt
      481 ccagtcggcg ccagagcatc gccagcacga tgcccgtgac gatgacccgc gcggggtaga
      541 gccagacgaa atccccagag aacgccagtg tcagcagcgt cgtcgccagc agaaaggtaa
      601 aaggaaccag cagaacgcct ggcatgccca ggcgggcgcc ggatcgaggt gccgttttcg
      661 tcgcgaagaa atgatgcctg tcagcgtaga ccagcatggc gacggccacc gtgatgaagg
      721 cgatccagcc ggcgaagctg tgaaatccgc cgacggcgac ggcgggggag aagccgttgc
      781 cgatgacgat caacgcgacg atccgcagaa cgttggcgca ccagatggcg atgatcccga
      841 tgggaaacag caacagggcg ttgggaaact tgaagtggtc ccggtagatg gcgagataga
      901 gcgtggtgaa aacggtgacc aggccgatgc cttcgtatcc tgagcagggc tccgcaattc
      961 ggctctgaaa gtagggcagg cccagtatca tccgctctcg atcgacgtaa acctcatcgt
     1021 cgatcagcca gagcacccat tctacagaag caaacgtcat ctggctcaac ggcccccaca
     1081 gctgctgaga tgacgcaccg atgaccagcg ccacggaggc gacgagcacg gaagccgcga
     1141 ttggtgtgcg ttcctggcgg accaggcgcc gccagtagga gaacggggcg atcagtgaga
     1201 gccagatgca aaccgtcgca gtggtggcca gaacccatgc gccgacgatg atcgcgtcgg
     1261 gcggggcgcc ctccgccgga tctgcaaaga tcaggtgcgt cagccagacc aggacggcgt
     1321 acgcggcaag ctgggcgagg agaagcgccc atggcgagcg gggcgccgtc acggtcacca
     1381 gatcgtcgta gtgggctttg agccgcgtgc caatcgtgac gagcagcgcg gccacgaagg
     1441 caatcgcgct cttaagaaag tagccgccgt aagcgagcca ggtgagccag ggactgtcgg
     1501 tagtttgcag cagcgtccag gcgtcgtagc ggtagctgat gaagaacatc tcgctcacga
     1561 gcagggcgct cactagagcg agctgcagcc cgaatagatt tggcgctttg cctgaaaggg
     1621 gaatgccgct ctgcatggtc agaagatagg agaaggcctg gttgatcgct agtctcactt
     1681 acaaactggt aacgtgcgac acaggttgct gggcttggct gaccacagat ctcctttctc
     1741 gggttgcggt cgcgcggagc tcttgcgtgc tgcgttatca tggggccgac gtgtacggaa
     1801 ccggcgagaa tctgtcctga acgaccgcct gaccatcgac tacttctccg acgtgctgtg
     1861 cgtgtgggcc tggatcgcgc agcggcgcat cgacgagatg aacgaccaat ggggcgagac
     1921 ggttcggctg ggcttccact gcgtcgacgt cttcggcgac gcaggcgcga aggtggcagc
     1981 gggttggagc gcccgtggcg gctacgaagg atttgcgcag cacgtgcgcg aggtcgccat
     2041 ccgctacgag gcgcctgccg tccacgagta tctctggacg aaggtcaggc cggtgacgtc
     2101 gggcaccgcc catctggtgc tcaaggcaac ggagattgcg gagagccccc tggagatgac
     2161 tcgcctgtct cgggccctgc gcgaggcgtt cttcgtcgat gctcgagacg tcggcgatct
     2221 cggcgtctgc ctggaggttg ctcaggggct tggtctcgcc gccgcggaac tgaagcgaca
     2281 cgtggattct ggcgcggcca tggccgccct tctggcggac tatcgcaagg cggaggagcg
     2341 gggcatcagg ggaagcccgt cctgggtgat gaacgagggt cgccaggtgt tgtacggcaa
     2401 cgtcggctac cgcattctga atgcaaatat cgaggagctg ctgcgccacc cggaacagga
     2461 agccagctgg tgctaggttg ctgcgcgttc gcgcccggcc acaagcgccg aggcgggccg
     2521 acgcgtaatg aggttccgtt gggaatccgc tag
//
LOCUS       DBNHKV010000093         9603 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_226853, whole genome shotgun sequence.
ACCESSION   DBNHKV010000093 DBNHKV010000000
VERSION     DBNHKV010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9603)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9603)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9603
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_226853"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="AC16PP_03010"
     CDS             <1..171
                     /locus_tag="AC16PP_03010"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009046132.1"
                     /note="catalyzes the formation of porphobilinogen from
                     5-aminolevulinate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR6480623.1"
                     /translation="TFAYQVSGEYAMHMAAVGNGWLDQSVVAESLLCLKRAGADGILT
                     YFAFTVAAALAD"
     gene            285..611
                     /gene="trxA"
                     /locus_tag="AC16PP_03015"
     CDS             285..611
                     /gene="trxA"
                     /locus_tag="AC16PP_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HIR6480624.1"
                     /translation="MSDNIVHTSDSDFDNDVLSSDKPVLVDYWAEWCGPCKMIAPILD
                     EIAEEYSDRIKVCKLDIDANQATPPKYGIRGIPTLMLFKNGEVEATKVGALSKSQLAA
                     FLDSNI"
     gene            855..2117
                     /gene="rho"
                     /locus_tag="AC16PP_03020"
     CDS             855..2117
                     /gene="rho"
                     /locus_tag="AC16PP_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR6480625.1"
                     /translation="MNLTDLKRKPVGDLIDMAREMGLENLARSRKQEVIAAIVRKQAK
                     EGEDIYGEGTLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDSIAGKIR
                     PPKDGERYFALLKVDEINFSEPNSKKHKILFENLTPLFPDDRLVLERGNGSTEDLTAR
                     IIDLIAPIGKGQRALIVSPPKAGKTLVLQNIAQSIAANNPEVVLIVLLIDERPEEVTE
                     MQRMVQGEVVASTFDEPPSRHVQVAEMVIEKAKRLVEHQKDVVILLDSITRLARAYNT
                     IVPSSGKVLTGGVDAHALERPKRFFGAARNIEEGGSLSIIATALVDTGSKMDEVIYEE
                     FKGTGNQELHLERKIAEKRVYPAINIRRSATRREELLMGEEELQRVWILRKLLHDMDD
                     IAAIEFMIDKLKDTKTNEEFFALMRGGR"
     gene            2129..2827
                     /locus_tag="AC16PP_03025"
     CDS             2129..2827
                     /locus_tag="AC16PP_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase domain-containing protein"
                     /protein_id="HIR6480626.1"
                     /translation="MQARIVRRTGAPVQEIVLQVEPDFAFAAGQYLEILLPNGARIPL
                     SIASGPYRLPDVHLHYRSTPGIAEAVLFDELLQTSQTLDVAGPFGDVRLELPLAGPIL
                     IAAGGTGASQALGMLDALAQAAPRGEVPFEVSFLWCADREADLYRRDWLASLNADWLS
                     TTLIADSRRDRDNRALAWLRAQSARFRDRRIFLCGGPQFVYAAADALQAGGVPRGQLA
                     ADAFGYAPRPDNSG"
     gene            complement(2824..4038)
                     /locus_tag="AC16PP_03030"
     CDS             complement(2824..4038)
                     /locus_tag="AC16PP_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HIR6480627.1"
                     /translation="MKTGIVLIAIAAVLGGLVGTLVVRDPGYVLLAYDDMAVETSLWF
                     ALVALVVIYFFVRLLVFAFSRLAATGGSFGAWNRQRQSKKAREQTIRGLLLMAEGEWA
                     EARRLLVLAAPRVDSPLINYLSAARGAHATGDVEGRDELLRKAHQSTPGARFAVGLTQ
                     AQLQASAEQWEQCLASLLQLQSESPRHQLVLSMLAECYEALQDWRALIELLPGIRRAK
                     LRDKAQLDELQKRAWSRRLAATTEDLTGLWAQMPKELRRNPELVAVLCDRLLEAGLAN
                     DAEAAVRGALEHEWRSELVQRYGVIVSDDVGRQLIVAEGWLKSRPNDSALLLALGRIC
                     LMNEQWAKAREYLEASLRLRHTPEVYGELGRLCVAMGDVDRGSQYLKQSVIGLPDLPL
                     PEGEPGAAAGTG"
     gene            complement(4035..5138)
                     /locus_tag="AC16PP_03035"
     CDS             complement(4035..5138)
                     /locus_tag="AC16PP_03035"
                     /inference="COORDINATES: protein motif:HMM:NF016275.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR6480628.1"
                     /translation="MDDDTSKGPAGDDAIGEPAPEATESASRTSGEAADQAAKAASAK
                     PRGRLLGGLGLLLALGALCGVGYLYYLLVFLGPSDELDARLGRLEASLPEIRRDLAAA
                     RDAQAEEIESAVAAQQASLDETRRALLGALNEVTAAAAPAPREWKIAEVEYLLRIANH
                     RVLMEGDVEAALQLMGAADVILEELDDFAFFQVRARLADEMLALKGVRGGDLQGVYLR
                     LEAIKTSIGELPVRLPDYFRPESSADASVEAEGFWSALAAELSGYFQLRRFDGATKPL
                     MAPEENAYLELNLRLAMERAQLAVLRRNQLVFERSLSSAREWILELLDPDDPSVEQVA
                     EELDALSTLKLERDLPDVSGSLTALLELRRGNP"
     gene            complement(5138..5839)
                     /locus_tag="AC16PP_03040"
     CDS             complement(5138..5839)
                     /locus_tag="AC16PP_03040"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIR6480629.1"
                     /translation="MRVWVTRTQPGADRQARRLRAAGHEPVVGPALRIVEVPGGAPRG
                     PFTDVVYLSEHAVRFGIARLTNQAWFSGARAHAVGAATAEHLAVAGIRAGFPEQASSE
                     GLLSLPEFADARGLTVLIVCGEGGRDLLETTLTARGARVEKLVVYRRRAVDEAPPDVG
                     SVDAIAVSSGDGFAAVARLWFAAGGRGDVPVLAPSPRVAGLGSALGFSNVYTCAGAGA
                     DALLKALESVSSTGR"
     gene            complement(5841..6758)
                     /gene="hemC"
                     /locus_tag="AC16PP_03045"
     CDS             complement(5841..6758)
                     /gene="hemC"
                     /locus_tag="AC16PP_03045"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIR6480630.1"
                     /translation="MSALRLVIATRQSQLALWQANFVSARLREAHPGLTVELLGITTR
                     GDRWLSSPLAEVGGKGLFIKELEEALASGTADVAVHSVKDVPAELPEGFAMPVIAYRE
                     DPRDVLVSRDGVTLAGLHAGARVGSSSLRRQAQLLARRPDLLIEPIRGNVDTRLRKLD
                     DGDYDALVLAAAGLARLGLAGRASEYLATDDCLPAAGQGALGIECRAGDGRVRALLEP
                     LEDAQVAACVCAERTVSAVLGADCTAPLAAHATPLGQGVMLQSRLATTDGRKILEARG
                     EGADPVAVGKSVAESLLAQGAAEVLGGGD"
     gene            complement(6755..7483)
                     /locus_tag="AC16PP_03050"
     CDS             complement(6755..7483)
                     /locus_tag="AC16PP_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003157680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR6480631.1"
                     /translation="MRVLIVDDEQLARDRLSRMVAELDDYEVAGEAGTGQDAVRLVAD
                     LQPEIILLDIRMPGMDGLEAARHLESVDEPPAVIFCTAYEEHAIEAFNLQAVGYLLKP
                     VRRENLRQALGNAQRVNKAQLAALSDDGSRRRSHISARTRKGIELVPLEAVRYFQADQ
                     KYVTVRHGEGELIIDETLRELEREFGELFIRVHRNALVAARHIIALDRLGDGHYQIRL
                     NDVDEHIDISRRHVASVRKFVRNL"
     gene            complement(7569..8609)
                     /locus_tag="AC16PP_03055"
     CDS             complement(7569..8609)
                     /locus_tag="AC16PP_03055"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6480632.1"
                     /translation="MADYSNHRERFVVPELCNAAAISLLVLFGELLVLVLLFAGGPIT
                     WLRLALMSLFVQWVVIMSAGALCWTRAWLSRFAVVPGAVLAFSMVMAITLFVGISADR
                     IMAPPGVEAINWMGILGQLLISGIITSLALRFLYVQQQLRAQEQSELQSRIQALQSRI
                     RPHFLFNSMNIIASLIATDPETAESVVEDLSELFRASLNDAGNQVPLQDELELCERYV
                     RIESLRLGDRLRVEWNVDDSGQDLKIPLLTLQPLLENAIYHGVQPLPEGGTITVHLWL
                     EGNDVNVRITNPIPEGEASNSSAGNRMAVNNIRSRLMVLYGTRAELRAAPEEGLFVTS
                     LKYPVSNMPLQT"
     gene            8635..>9603
                     /gene="argH"
                     /locus_tag="AC16PP_03060"
     CDS             8635..>9603
                     /gene="argH"
                     /locus_tag="AC16PP_03060"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR6480633.1"
                     /translation="MSRDSKPWGGRFSEATDAFVESFTASVAFDKRLYHHDIRGSIAH
                     ANMLSRIGVLTEGERNEIVSGLQAIHKEIDEGELAWRVELEDVHMNVESRLIELIGET
                     GKKLHTGRSRNDQVATDIRLYLKDAIDELVALETQLLGALVDLAEAHADTVMPGFTHL
                     QPAQPVTFGHHLLAWFEMLHRDRDRLNDCRRRVDVMPLGSAALAGTTYPIDRAGVAAE
                     LGFGAVAENSLDAVSDRDFAIEFTSVAALTMNHFSRWSEELVLWTSQQFGFVELPDRF
                     CTGSSIMPQKKNPDVPELIRGKSGRVAGNLVALLTLMKGQPLAYNKD"
BASE COUNT         1747 a         3267 c         3028 g         1561 t
ORIGIN      
        1 accttcgcct atcaggtcag cggcgagtat gcgatgcaca tggccgccgt cggcaatggc
       61 tggctcgacc aatccgtcgt cgccgagtcg ctgctctgcc tgaaacgagc gggcgccgac
      121 ggcattctga cctacttcgc gttcaccgtc gccgcagccc tcgcggatta gtggccgccc
      181 gccgatgtcg tgataaactg ccaccgttcc gccacggccc tgggccctct ccaagcgtcg
      241 cgtcccagac cgggtgactg tcatatcatt actggagcaa attgatgagc gacaacatcg
      301 ttcacaccag cgattccgac ttcgacaacg acgtgctctc ttccgacaag cccgtcttag
      361 tggattactg ggcggagtgg tgcggcccct gcaagatgat cgcgcccatt ctcgacgaga
      421 tcgccgagga gtactccgac cgcatcaagg tctgcaagct agacatcgac gccaaccagg
      481 ccacgccgcc caagtacggc atccgtggca tacctaccct catgctgttc aagaacggcg
      541 aggtcgaagc gaccaaggtg ggcgcgttgt cgaagtctca gctggccgcc ttcctggaca
      601 gcaacatctg atatccacct atcggccccg tcaggcggcg cgtaaacggg ttcacaaacc
      661 cggattgtgc gaccaaaggg gtgcttgacg gggtctttca atacactaaa cttggctgca
      721 atcgctggag tcgaactcca gcgcccaccc gccgccaccg cgcttcgccg gtcgcagcac
      781 ccaaagaaca gccataaaaa tctaagaaca gtccgttcgt cgtttttaca acctgccggt
      841 ttgcgcttat gcccatgaac cttacagacc tgaagagaaa acccgtcggc gacctgatcg
      901 acatggcccg cgagatgggg ctggagaacc ttgcgcgttc gcgcaagcag gaggtcatcg
      961 ccgccatcgt ccgcaagcaa gccaaagagg gcgaggacat ctacggggag gggaccctgg
     1021 aaattctcca ggacggcttc ggttttctgc gatccgcaga cagttcctat ctcgcggggc
     1081 ccgacgacat ctacgtctcg cctagccaga tacgccgatt caacctgcga acgggcgaca
     1141 gcattgccgg caagatccgg ccgcccaagg acggcgagcg ctacttcgct ctgctgaagg
     1201 tcgacgagat caatttcagc gagcctaact ccaagaaaca caagattctt ttcgaaaacc
     1261 tcaccccgct cttccccgac gatcggctgg tgctcgagcg cggcaacggc agcaccgagg
     1321 atctgaccgc gcgcatcatc gacctcattg cgcccatcgg caaaggccag cgagcgctca
     1381 tcgtctctcc cccgaaagcg ggaaagacgc tggttctgca gaacatcgct cagtccatcg
     1441 cggccaacaa ccccgaggtc gtgctgatcg tgctgctcat cgacgagcgg cccgaggaag
     1501 tcacggaaat gcagcgcatg gttcagggcg aggtggtggc gagcacgttc gacgagcccc
     1561 cctcccgtca cgtccaggtg gcggagatgg tcatcgagaa ggcaaagcgg ctggtggagc
     1621 accagaagga cgtcgtgatt ctgctggatt cgatcacccg tctcgcaagg gcctacaaca
     1681 ccatagtgcc gtcgtccgga aaggttctga ctggcggcgt cgacgctcac gcgctggagc
     1741 gccccaagag attcttcggt gcggcccgaa acatcgaaga gggcggcagc ctgtccatca
     1801 tcgccaccgc cctggtggat accggctcca agatggacga ggtcatctac gaggagttca
     1861 agggaacggg caaccaggag cttcacctgg aacgcaagat cgcagaaaag cgcgtctacc
     1921 cggccatcaa catccgccgc tcggccaccc gccgcgagga gctgctgatg ggcgaggaag
     1981 agcttcagcg ggtctggatc ctgcgcaagc tgctccacga catggacgac atcgccgcca
     2041 tcgagttcat gatcgacaag ctgaaggaca cgaagaccaa cgaggagttc ttcgcgctga
     2101 tgcgcggcgg tcgctgaccg agccaaccgt gcaggccagg atcgtccgcc gcacgggcgc
     2161 acccgtgcag gagatcgtcc tgcaagtaga gccagacttc gctttcgctg cgggccagta
     2221 cctggaaata cttcttccga acggcgcgcg cattccactt tccatcgcct caggtcccta
     2281 ccgcctcccc gacgtgcacc tgcattaccg atccaccccc ggcatcgccg aggccgtcct
     2341 tttcgatgag ctgctccaaa cgagccaaac cctcgatgtc gccggccctt ttggcgacgt
     2401 ccggctggag ttgcctctgg ccggcccgat tctcatcgcg gcagggggaa cgggcgcgag
     2461 ccaggcgctc ggcatgctcg atgcgctcgc gcaggcagcg ccgcgcggtg aggtcccgtt
     2521 cgaggtctcg tttctctggt gcgcggaccg ggaggctgat ctgtatcgca gggactggct
     2581 tgcctcgctg aacgcggact ggctgtctac cacgctgatc gcggattcga gacgggatcg
     2641 ggacaatcgg gcgcttgcct ggctgcgggc gcaatcggcg cggttccggg atcggcggat
     2701 cttcctgtgc gggggcccgc agttcgtcta cgcagcggcc gacgcgctcc aagccggcgg
     2761 tgtgccccgc gggcaattgg ccgccgatgc gttcggctac gcgccccgtc cggacaactc
     2821 gggctagccg gtccccgccg ccgcgcctgg ctcgccttcc ggcagcggca gatccggcag
     2881 cccgatcacc gactgcttga gatactggct gccgcggtcc acgtcgccca tggcgacgca
     2941 caggcggccc agctcaccgt agacctcagg cgtgtgccgc aggcgcaggc tcgcttcgag
     3001 gtactcgcgg gccttggccc actgctcgtt catcaggcag attcgaccca gggccagcag
     3061 cagcgccgaa tcgttgggcc gggacttcaa ccacccctcg gccacgatga gctgcctgcc
     3121 cacatcatcc gagacgatca caccgtaccg ctggaccagc tcgctgcgcc actcgtgttc
     3181 cagagcgccc cgaaccgcgg cctcggcatc gttcgcaagc cctgcttcaa gcagccggtc
     3241 gcagagcacg gcaacgagct ccgggttacg gcgcagctcc ttgggcatct gcgcccaaag
     3301 acccgtgagg tcttccgtgg tcgccgcgag ccgccggctc caggcgcgct tttgcagctc
     3361 gtcgagctgg gccttgtcac gcagctttgc gcgccggatg cccggcagaa gctcgatgag
     3421 agcccgccag tcctgcagcg cctcgtaaca ctccgcaagc atagacagca ccagctgatg
     3481 acgcggcgac tcggactgca gctggagaag cgaagccagg cattgctccc actgctcagc
     3541 cgaagcctgc agctgcgcct gggtgagccc cacggcgaat cgcgcccccg gtgtcgactg
     3601 gtgggccttg cgaagcagct cgtcgcggcc ttcgacgtcg cccgtggcgt gggcgccccg
     3661 agcggcgctc aagtagttga tcaggggcga atccacccgt ggggcggcca gaacgagcag
     3721 ccgccgcgct tcggcccact ctccctcggc catgagcagc agcccgcgaa tcgtctgttc
     3781 acgcgccttc ttcgactgcc gctggcggtt ccatgcgccg aagctgccgc ccgtggcggc
     3841 cagccgtgag aatgcgaaaa caagcagacg cacgaagaaa tagatgacga ccagagcaac
     3901 aagcgcgaac cagagactgg tctcgacggc catgtcgtca taagccagca gcacgtaccc
     3961 cgggtcacgc accaccagcg tgccgaccag cccgcccagc acggcggcga tggcgatcag
     4021 cacaatgccc gtcttcacgg gttgccccgg cggagctcga gcagcgcggt gagggagccc
     4081 gacacgtcgg gcagatcccg ctcgagcttg agcgtgctca gcgcgtccag ctcctcggca
     4141 acctgctcga cgctcggatc gtcagggtcg agtaactcga gaatccattc gcgggcgctg
     4201 gagaggctcc gctcgaagac gagctgattg cggcgcagaa cggcgagttg ggcacgctcc
     4261 atggccagcc gcaggttgag ctctaagtaa gcgttctctt ccggcgccat gagcggcttg
     4321 gtcgcgccgt cgaatcggcg cagctgaaaa tagccggaaa gctccgcggc cagcgcgctc
     4381 cagaagcctt cagcctccac agacgcgtct gcagagctct ccggtcggaa gtagtcgggc
     4441 agccggacgg gcaactcgcc gatgctggtc ttaatggctt ccaggcgaag gtaaacgccc
     4501 tgcaggtcgc cgccgcggac gcccttgagc gccagcatct cgtcggccag ccgggcgcga
     4561 acctgaaaga aggcaaaatc gtcgagctcc tcgagaatga catcagcagc ccccatgagc
     4621 tgcagggcgg cctccacgtc accctccatg agcacccgat ggttggcaat cctgagcagg
     4681 tactccacct cggcgatctt ccattcccgg ggggcggggg ccgcggcggc ggtgacctcg
     4741 ttgagcgccc cgaggagcgc tctacgggtc tcgtccagtg acgcctgctg agcggcgacg
     4801 gcgctctcga tctcctcggc ctgcgcgtcg cgcgccgccg cgagatctcg gcggatctct
     4861 ggaagagacg cctccaggcg cccaagccgg gcatcgagct catccgacgg tcccaggaag
     4921 acgaggaggt aatagagata gccgaccccg caaagcgcgc ccagggcgag caggaggcca
     4981 agcccgccga gaaggcggcc gcgcggtttt gccgacgctg ccttcgcggc ctggtcagct
     5041 gcctcccctg aggtcctgga agcggattct gtcgcctccg gggcgggctc accgatggcg
     5101 tcgtcgccgg ccggaccttt gctggtatcg tcgtccatca cctacccgtc gaactcacgg
     5161 actcgagcgc ctttagcagg gcatcggcgc cggctcccgc acatgtgtat acgttactga
     5221 accccaatgc ggaacccagc ccggccaccc gtggggacgg cgccaacacc ggcacgtccc
     5281 cgcgaccgcc tgcggcaaac cataaccgcg ccaccgccgc gaatccatcg ccgctggaga
     5341 cggcgatggc gtccaccgag ccgacatccg gaggggcctc gtccacggcg cggcgccggt
     5401 agacgacgag cttttccacc ctggcgcccc ttgccgtcaa cgtcgtctcg agcagatccc
     5461 gccctccttc tccgcagacg atgagcaccg tcaggccccg ggcatcggca aattccggca
     5521 gcgagagcag cccctcggag ctcgcctgct cagggaagcc cgcacgaatc ccggcaacgg
     5581 ccaggtgctc ggcggtcgct gctcccacgg catgggcgcg cgcaccggag aaccacgcct
     5641 ggttggtcag gcgcgcaatg ccaaaccgaa cggcatgctc ggaaagatag accacgtcgg
     5701 taaaaggtcc gcgaggcgcg ccgccgggca cctccacgat tctcagcgcc gggcccacga
     5761 ccggctcgtg gccggccgct ctcagccttc gggcctggcg gtcggcgccg ggttgggttc
     5821 gggtcaccca gacgcgcaac tcaatctccg ccgccgagca cctcggccgc gccctgcgcc
     5881 aaaagcgact cggcgaccga tttgcccacg gctaccggat cggccccctc gccacgggct
     5941 tccagaatct tccggccgtc ggtggtcgcc agacgggatt gcagcatcac gccttggccg
     6001 agcggcgtgg cgtgtgcggc cagcggggcc gtgcaatccg ctcccagcac ggcgctcacc
     6061 gtgcgctcgg cgcaaacgca ggcagcaacc tgagcatctt cgaggggttc gagcagtgcc
     6121 cgcacgcgcc cgtcgcccgc ccggcactcg atgcccagcg ctccctggcc ggcggcgggc
     6181 aggcagtcgt cggttgccag gtactcgctg gcgcgccccg ccagtcccag ccgggcgaga
     6241 cccgctgcgg ccagcaccag ggcgtcgtaa tcgccgtcgt ccagcttgcg cagccgggtg
     6301 tccacgttgc cccggatggg ctcgatcagc agatcggggc gccgggccag gagctgagcc
     6361 tgcctgcgca ggctcgatga gccgacccgg gcaccggcgt gcaacccggc cagagtcacc
     6421 ccatcccggc taaccagcac gtcacgggga tcctcacgat aggcgatgac cggcatggcg
     6481 aatccttcgg gaagctccgc cggcacgtcc tttaccgagt gcacggcgac gtcggccgtg
     6541 cccgaggcaa gcgcttcttc gagctccttg atgaacaggc ccttgccgcc gacctcggcg
     6601 aggggcgaag aaagccatcg gtccccacgg gtggtgatgc cgaggagttc cacggtgagg
     6661 cccggatggg cctcgcgaag acgcgcgctg acgaagttgg cctgccacaa ggcaagctga
     6721 ctctgccgcg tggctatgac gagccgtaat gcgctcacag gttcctgaca aacttgcgca
     6781 cggaggccac gtggcgccgg ctgatgtcga tgtgctcgtc gacgtcgttg aggcggatct
     6841 ggtagtggcc gtcgcccaga cgatcgagcg cgatgatgtg gcgcgcggcc accagggcgt
     6901 tgcgatggac gcggatgaac agctcgccga actcgcgctc tagttcgcga agggtttcgt
     6961 cgatgatcag ctcgccctcg ccgtggcgca cggtgacgta cttctgatcg gcctgaaagt
     7021 agcgcacggc ttcgaggggt acgagctcga tgcccttgcg cgtgcgcgcg gagatatggg
     7081 agcggcgtcg gctgccgtca tccgagagcg ctgccagctg agctttgttg acccgctgag
     7141 cgttgccgag cgcttggcgc aggttctccc ggcgcacggg tttcagaagg taaccgacgg
     7201 cctggaggtt gaacgcctcg atggcgtgct cctcgtaggc cgtgcagaag atgacggccg
     7261 ggggctcgtc gaccgactcc agatgacgcg ctgcttccag cccgtccatg cccggcattc
     7321 ggatatcgag caggatgatc tccggctgca ggtcagccac caggcgcacg gcgtcctgcc
     7381 cggtgccagc ctcaccggct acctcgtaat cgtccagctc ggccaccatt cgcgacaggc
     7441 gatctcgggc cagctgttcg tcgtccacga tgagaaccct gatgctcata tcccctccct
     7501 gagcgtgctg gtctccaggc tcgccgccgc gctcgccgcc gcgcttgccg ccgcgctcgc
     7561 cgccgcgctc atgtctgcaa cggcatgttc gacaccgggt acttcagcga cgtgacgaac
     7621 agcccttcct cgggcgccgc cctcaattcc gcacgggtac cgtacaacac catcagcctg
     7681 ctgcgaatgt tgttgactgc cattcggttt cccgcgctgc tgttgctcgc ctcgccctcc
     7741 ggtatcgggt tggtgatgcg aacgttgacg tcattcccct ccagccacag atgaacggtg
     7801 atcgtgccgc cttccggcag cggctggacc ccgtggtaga tggcattttc cagaagtggc
     7861 tgcagagtca gcagaggaat cttgagatcc tggcccgagt cgtccacgtt ccattcgacg
     7921 cgcagccgat caccgagccg cagggactcg atgcgcacgt aacgctcgca aagctccagc
     7981 tcgtcctgca gcggcacctg atttcccgcg tcgttgaggc tggcacgaaa gagctcggag
     8041 aggtcctcca cgacagactc cgcggtctcg gggtcggtgg cgatgaggct tgcgatgatg
     8101 ttcatgctgt tgaacaggaa gtgcgggcga atgcgcgact gcaacgcctg gatgcgcgac
     8161 tggagctccg actgctcctg agcgcgcagc tgctgctgga cgtacaggaa gcgcagcgcc
     8221 agggaagtga tgatcccgga aatgaggagc tggccgagaa tgcccatcca gttgatcgct
     8281 tccacgccgg gcggagccat gatccgatcg gcgctgatcc cgacgaacag ggtgatcgcc
     8341 atgaccatcg agaaagcgag cacggcgcca ggcaccaccg cgaaccggga gagccaggct
     8401 cgcgtccagc acagggctcc cgcgctcatg atgacaaccc actgcacgaa cagggacatg
     8461 agcgcgagcc ggagccacgt gatggggccg ccggcgaaca gcagcaccag aaccagaagc
     8521 tcaccgaaga gcacgagcag ggaaatggcc gccgcgttgc aaagctcggg gaccacgaac
     8581 cgctcgcgat ggttgctata atcggccatc cgctaaagga accctcttgt caccatgagc
     8641 cgagattcaa aaccctgggg cggccgcttt tcggaagcca ccgatgcgtt cgtcgaatcg
     8701 ttcacggcct cggtggcatt cgataagcgt ctttaccatc acgatatcag gggctccatc
     8761 gcccacgcca acatgctgag ccggatcggc gtgctcaccg aaggtgagcg caacgagatc
     8821 gtatcgggat tgcaggcgat tcacaaagag atcgacgaag gcgagctcgc ctggcgggtt
     8881 gagctcgagg acgtccacat gaacgtggag tcgcgcctta tcgagctcat tggagaaacc
     8941 gggaaaaagc tgcacacggg ccggtcccgc aacgaccagg tcgccaccga tatccggctc
     9001 tacctgaagg acgccatcga cgagctggtc gctttggaaa cccagctgct cggcgcgctg
     9061 gtggacctgg ccgaagccca cgcggacacc gtcatgcccg gtttcacgca tctgcagccg
     9121 gcgcagcccg taacgttcgg ccaccatctg ctggcgtggt tcgagatgct tcaccgcgac
     9181 cgcgaccgcc tcaatgactg ccgccggcgc gtggacgtca tgcccctggg cagcgcggcg
     9241 cttgccggca ccacctatcc catcgatcgg gccggtgtcg ctgccgagct cggattcggc
     9301 gccgtggccg agaactcgct ggacgcggtg agcgaccggg acttcgccat cgagttcacg
     9361 tcggttgccg cgttgacgat gaaccacttt tctcgctgga gcgaggagct ggtgctgtgg
     9421 acgtcccagc agttcggatt cgtcgagctg cccgatcgtt tctgcacggg ctcgagcatc
     9481 atgccgcaga agaagaaccc cgacgtcccc gagctgatcc ggggcaagtc gggcagggtg
     9541 gccggcaacc tggtggctct gctgacgctg atgaaaggcc agccgctggc ctacaacaag
     9601 gac
//
LOCUS       DBNHKV010000094         6301 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_509941, whole genome shotgun sequence.
ACCESSION   DBNHKV010000094 DBNHKV010000000
VERSION     DBNHKV010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_509941"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="AC16PP_03065"
     CDS             <1..333
                     /locus_tag="AC16PP_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HIR6480634.1"
                     /translation="KTIDRVIDVFPAEEKSMVRSMLSESLQSVISQTLLKRVNGGRVA
                     AHEIMIGTAAIRNLIREDKVAQMYSAIQTGSSVGMQTLDQCLLDLVQKRTITRDSARE
                     KAKNPDDF"
     gene            351..1490
                     /locus_tag="AC16PP_03070"
     CDS             351..1490
                     /locus_tag="AC16PP_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HIR6480635.1"
                     /translation="MQFEKLLKLVVEKGASDLFITAGKPPMLKVNGKLFAAGTTNLSA
                     DQSRDVVHSIMDEDQKKQFHDHHECNFAIHSEGLGRFRVSAFQQRNLAGMVLRRIEVN
                     IPLVSDLGLPPILEDLSMTRRGLIIFVGATGTGKSTSLAAMIGHRNEHSSGHIISIED
                     PIEFVHQHRGCVVTQREVGVDTESFEVALKNTLRQAPDVIMIGEVRTRETMEHAVAFA
                     ETGHLCLCTLHANNANQALDRIIHFFPSDRHSQVWMDLSLNLRAMVAQQLVPTVDGQG
                     RRAAIEILINTPLMADHIRKGEVHLLKELMSKSTEHGMQTFDQALYQLYSEGEISHEA
                     ALQHADSPNDLRLMIKLGSDANPDLMGQVEGLTIEEDEPAGTFLR"
     gene            complement(1512..1901)
                     /gene="ruvX"
                     /locus_tag="AC16PP_03075"
     CDS             complement(1512..1901)
                     /gene="ruvX"
                     /locus_tag="AC16PP_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR6480636.1"
                     /translation="MKQGHVLAFDFGLKHIGVAVGQSVTRTASPLITIAARDGTPDWS
                     DVERLITEWQPAVLLVGLPLNMDDSESEMSLKARGFAERMGRRFGVPIELVDERLTSF
                     AARESGSDRPHEAAATMIAESWLAADG"
     gene            complement(1909..2469)
                     /locus_tag="AC16PP_03080"
     CDS             complement(1909..2469)
                     /locus_tag="AC16PP_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003297209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR6480637.1"
                     /translation="MAADTSLKNQFLLAMPGLAGTYFGDTVTYVCEHSADGAMGLMVN
                     RPTEVTLQALLADLGIDSSAVSADIPVMEGGPVASERGFVLHSDDRAFDSSLSLGDGL
                     MLNSTREVLEAIAAGEGPADFLVAMGYAGWGGGQLEQELLENAWLTCPGNTDVLFRTP
                     FAERVNQAAAALGIDFRLMSSQAGHA"
     gene            complement(2459..3319)
                     /locus_tag="AC16PP_03085"
     CDS             complement(2459..3319)
                     /locus_tag="AC16PP_03085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6480638.1"
                     /translation="MSAAAITPRDRLGFTLFLAVSLHAAIILGVSFTADLDLLESPTV
                     EVTFAQHSDLEAPEDADFIAQTNQLGSGTESEVLETTTDQEAEFFANEFADAISNPLP
                     TIEQNQLDSRELVTTISEVDDVAPIEEETPVEENVNPQPATDIRFDDLAKEIASLEAR
                     IAADQQAKAKQPRVKRLTSVSAKSADEAAYLNMWRQKVERIGNANYPDSRVYGNLRML
                     VVLSYDGTLKDVRILESSGHKSLDDAALRIVRLAAPYQQFPVEMRKKYDQLEIIRTWK
                     FSRSGARLGS"
     gene            complement(3344..4330)
                     /gene="gshB"
                     /locus_tag="AC16PP_03090"
     CDS             complement(3344..4330)
                     /gene="gshB"
                     /locus_tag="AC16PP_03090"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004419666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HIR6480639.1"
                     /translation="MSLKIAFVMDPLPTLSLKKDSTLAMIRAAQLKGWQVGYLEQGDL
                     LLHEGAPCGMLRSLELVGPFADSLDPAEAGDRWYQLSEAALTPLAELDVIMMRKDPPF
                     DMEYIYSTYLLERAEALGVLVVNKPQGLRDCNEKFFATAFQECCPPLVVTRSEAALRE
                     FQRTHGNVVFKPLDGMGGQGVFRIMEGDENLGVIIETLTKGGTAQIMGQKFIPEIASG
                     DKRVLLIDGEPIPYALARIPMAGEARGNLAAGGTGEGRELTERDRWIAAQVGPELRRR
                     GLLFVGMDVIGDYLTEVNVTCPTCIRELDAQFSLNIGGMLMDTISAKLDAQA"
     gene            4586..4966
                     /gene="pilG"
                     /locus_tag="AC16PP_03095"
     CDS             4586..4966
                     /gene="pilG"
                     /locus_tag="AC16PP_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HIR6480640.1"
                     /translation="MEQELKGVKVLVIDDSKTIRRSAENLLTKEGYDVITATDGFDAL
                     AKIVDSRPGIIFVDVMMPRLDGYQTCALIKNNSEFKTTPVVMLSSKDGLFDKAKGRIV
                     GSDQYITKPFSKDELLGTIRNLVH"
     gene            4992..5357
                     /locus_tag="AC16PP_03100"
     CDS             4992..5357
                     /locus_tag="AC16PP_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010795388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6480641.1"
                     /translation="MARILVVDDSPSETHQLANALSRHGHQVLTAASGSDGISLANEE
                     QPDIILMDVVMPGINGFQATRQLTRSQGTSHIPVIIVSSKSQDADRVWGKRQGAQGYL
                     TKPVDDRTLISTVNEALSA"
     gene            5366..5908
                     /locus_tag="AC16PP_03105"
     CDS             5366..5908
                     /locus_tag="AC16PP_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR6480642.1"
                     /translation="MNGQAQNILTDIRATTIANAAPLPSRENEHLEWQGVGYQLGGVR
                     LVSPMGEISEILAVPKFATLPGVKPWMLGVANVRGRLIPIIDLHNFLDMRTTTPMVQW
                     RVLVVEDEELIAGFVVEQSLGIQHFLEDSFEESTAEEVAALKPYLRGAFHQGGCVFYE
                     AHLKSILRDEKFFDVADKTN"
     gene            6053..6301
                     /locus_tag="AC16PP_03110"
                     /pseudo
     CDS             6053..6301
                     /locus_tag="AC16PP_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520133.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
BASE COUNT         1237 a         1924 c         1950 g         1190 t
ORIGIN      
        1 aagaccatcg accgggtcat cgacgtgttt cccgcagaag agaagtccat ggttcggtcc
       61 atgctctcgg agtcgctgca gtcggtgatc tcgcaaacgc tcctcaagcg ggtcaacggc
      121 gggcgcgtgg ccgctcacga gatcatgatc ggcacggcgg ccatccggaa cctgattcgc
      181 gaggacaagg tcgcgcagat gtactcggcc atccagaccg gcagctccgt aggcatgcag
      241 acgctggatc agtgcctgct cgatctcgtt cagaagcgca cgatcacccg cgactcggct
      301 cgcgagaaag ccaagaaccc ggatgacttc taacggcgga ggttagcgcc atgcaattcg
      361 aaaagctgtt gaaactggtg gtggaaaaag gcgcgtcgga ccttttcatc accgccggca
      421 agccgcccat gctcaaggtc aacgggaagc tgttcgccgc tggaaccacc aacctgtctg
      481 ccgatcagtc ccgggatgtc gtgcacagca tcatggacga ggaccagaag aagcagtttc
      541 acgaccatca cgagtgcaac ttcgccattc attccgaagg gctgggtcgt tttcgggtga
      601 gcgcgttcca gcagcgcaac ctggcaggca tggtgctgcg gcgcatcgag gtcaacatcc
      661 ctctggtctc cgatctgggc ctgccgccca tcctggagga cctgtcgatg acgcggcgcg
      721 gcctcatcat tttcgtgggg gcgaccggca ccggtaagtc cacgtcgctg gccgccatga
      781 tcggtcatcg caacgagcac tcctcgggtc acatcatcag catcgaggac cccatcgaat
      841 tcgtgcacca gcacaggggc tgcgtcgtga cccagcgcga ggtgggcgta gacacggaaa
      901 gcttcgaggt cgccctcaag aacacgctgc gccaggcgcc ggacgtcatc atgattggtg
      961 aggtgcggac ccgggaaacc atggagcacg cggtggcctt tgcggaaacc ggtcacctct
     1021 gcctctgcac cctccacgcc aacaacgcca accaggcgct ggacaggatc attcacttct
     1081 tccccagcga ccgtcactcc caggtgtgga tggatctttc tctcaacctg cgcgccatgg
     1141 tggcccagca gctcgtcccc acggtggacg gacagggacg gcgggcagcg atcgagatac
     1201 tgatcaacac gccgctgatg gccgaccaca tccgtaaagg tgaggtgcat ctgctcaaag
     1261 agctgatgtc caaatccacc gagcacggca tgcagacgtt cgaccaggcg ctctatcagc
     1321 tctacagcga gggcgagatc agccacgagg ctgcgctgca gcatgcggac tcgccgaacg
     1381 acctgaggct gatgatcaag ctgggttccg atgcaaaccc ggacctgatg ggtcaggtcg
     1441 aagggttgac catcgaagaa gacgagccgg cgggtacctt cctgcgctga gagccgctct
     1501 tccccagcgg ttcagccatc ggccgccaac caggattccg cgatcatcgt tgctgcggcc
     1561 tcgtgaggcc gatcgctgcc ggattcgcgc gccgcaaacg aagtcagccg ctcgtcgacc
     1621 agctcgatgg gaacgccgaa ccggcggccc attcgctcgg cgaacccgcg agccttgagc
     1681 gacatttcgc tctcgctgtc gtccatgtta aggggtaggc ccaccagcag caccgccggc
     1741 tgccactcgg taatcagccg ttccacgtcc gaccagtccg gcgttccgtc cctggccgcg
     1801 atggtgatca gcgggctggc cgttcgggta accgattggc caacggccac gccgatgtgc
     1861 ttcagcccga agtcgaacgc cagcacgtga ccctgcttca cgcggctgtc aggcgtggcc
     1921 cgcctgtgag gacatcagcc ggaaatcgat gcccagcgcg gcagccgcct ggttcacgcg
     1981 ttctgcgaaa ggcgtgcgaa agaggacgtc ggtgtttcca gggcaggtga gccaggcgtt
     2041 ttccagcagc tcctgctcca gctggccccc gccccagccg gcgtatccca tggcgaccag
     2101 gaagtccgcg ggcccctcgc cggccgcaat ggcctcgagc acctcgcggg tggagttgag
     2161 catcagcccg tcccccagag agagcgaaga gtcaaaggct cggtcgtcgc tgtgcagcac
     2221 gaatccgcgc tcgctcgcca cgggcccccc ttccatcacg ggtatgtccg ccgacacggc
     2281 gctggagtct atgccgagat cggccagcag cgcctgcagc gtaacttccg tcgggcgatt
     2341 gaccatcagg cccatggcgc cgtcggcgct gtgctcgcat acgtaggtga ccgtgtcccc
     2401 gaaataggtg ccagccaggc cgggcatggc gagcaggaac tggtttttca ggctggtatc
     2461 agctgccaag gcgggctccg gagcgtgaga atttccaggt gcgaatgatc tccaactgat
     2521 cgtacttctt gcgcatctcc accggaaact gctggtaggg cgcagccaga cggacgattc
     2581 gaagcgcggc gtcgtcgagg gatttgtgcc cggaagactc cagaatccgg acgtccttga
     2641 gcgtgccgtc gtagctcagc accacgagca tccgcagatt gccgtacacc cggctgtcgg
     2701 gatagttggc gttgccgatg cgctcgacct tctgacgcca catgttcagg taggcggctt
     2761 cgtctgctga cttggcggac acgctggtga gccgcttcac ccttggctgc ttcgccttgg
     2821 cctgctggtc cgcggcgatc ctggcttcca ggctcgcgat ctccttcgcc agatcgtcga
     2881 agcgaatgtc ggttgccggc tgcggattga cgttttcctc caccggcgtc tcttcttcaa
     2941 tgggcgccac gtcgtctacc tcggagatgg tcgtcaccag ctcccgagag tcaagctggt
     3001 tctgctcgat ggtcggcaag gggttggata tcgcgtcggc gaactcgttg gcgaaaaact
     3061 ccgcttcctg gtccgtcgtg gtctccagca cttcgctctc ggtgccgctg ccgagctggt
     3121 tggtctgagc gatgaagtcg gcatcctcgg gtgcttcgag gtcgctgtgc tgggcaaacg
     3181 tcacctcgac cgtagggctt tcgagcaggt cgaggtctgc cgtgaagctg acgccgagaa
     3241 tgatggcggc gtgcagcgag acggccagaa acagggtgaa gcccagacgg tctcgtggag
     3301 tgatggcggc ggcgctcatg tttgcagggt gtcgacgggg cgttcaggcc tgagcatcta
     3361 gcttggcaga tatcgtgtcc atgagcatac cgccgatatt cagcgaaaac tgtgcatcga
     3421 gttcccgtat gcaggtcggg caggtgacgt ttacctccgt gagatagtcg ccgatgacgt
     3481 ccatgcccac gaacagcagg ccccgccgac gcagctcggg accgacctga gcggcgatcc
     3541 atcggtcgcg ctcggtgagc tcccgtccct cgcccgtgcc gcccgccgcc aggttgcctc
     3601 tcgcctcgcc ggccatgggg attctggcca gggcgtaggg gatcggctcg ccatcgatga
     3661 gcagaacgcg cttgtccccg ctggcaatct ccggaatgaa cttctggccc atgatctggg
     3721 cggtgccgcc tttcgtgagc gtctcgatga tgacgcccag gttctcgtcg ccctccatga
     3781 tgcggaacac gccctggccc cccatgccgt caaggggctt gaacaccacg ttgccgtgag
     3841 tccgttgaaa ctcgcgcagc gccgcttcgg accgggtcac gaccagtggg gggcaacatt
     3901 cctggaaggc ggtggcaaag aacttctcgt tgcagtcgcg cagcccttgg ggtttgttga
     3961 ccaccagcac gcccaacgct tcggcccgtt cgaggaggta ggtggagtag atgtactcca
     4021 tgtcgaacgg cgggtctttg cgcatcatga tcacgtccag ctcggcgagc ggggtaaggg
     4081 ccgcttcgct gagctgatac cagcgatcgc cggcctcggc cggatcgagg gaatcggcaa
     4141 acggcccgac gagctccagg gatcggagca tcccgcaggg cgcgccctca tggagcagca
     4201 ggtccccctg ctcgagatag cctacctgcc accccttgag ctgggctgcg cggatcattg
     4261 ccagcgtgct gtccttcttc agcgacaggg tgggcagagg gtccatcacg aaggcgattt
     4321 tgagactcat gggttctgtg gtgctccggg cgagccggga cagaagttgc tccggctggg
     4381 gcggtaacta gcttggcggt cgtagcgtaa ccccaaaaat gagaacgaat tccctttctt
     4441 atccaacgct tatagatcgt ttcgggacaa tcatgataca acgggagcgt tcggtaggta
     4501 cgtgaaaatc ctgccgacag ccttgatgac gccatactca atggacggtg acacgacggt
     4561 caagtcggaa gtagaaggac cagatatgga acaggaactc aaaggcgtta aggtgctggt
     4621 aatcgacgac agtaagacca ttcgtcgaag cgccgagaat ctgctgacca aggaagggta
     4681 tgacgtcatc accgccacgg acggctttga cgcgctcgcg aaaatcgtcg attcacggcc
     4741 gggaatcatc ttcgtcgacg tcatgatgcc tcgtcttgac ggttaccaga cctgcgcgct
     4801 catcaagaac aacagcgaat tcaagactac ccccgtagtc atgctctcga gcaaggacgg
     4861 cctcttcgac aaagccaaag gccggatcgt gggttcggat cagtacatca ccaagccctt
     4921 cagcaaggac gagctgctcg gcacgatccg gaacctcgtc cattaggaag caggcacagg
     4981 acgcgcagaa gatggcaagg attctcgtag tcgacgactc tccctcggag acgcaccagc
     5041 tggccaacgc gctttcgcgt catggtcacc aggtgctgac ggctgcctcc ggcagcgacg
     5101 ggatcagtct ggccaacgaa gagcagcctg acatcattct gatggacgtc gtgatgcctg
     5161 gtatcaacgg ctttcaggca acgcggcagc tcacgagaag ccagggtacc agccacattc
     5221 cggtgatcat cgtctcctcc aagagccagg atgcggaccg ggtctggggc aaacgccagg
     5281 gcgcgcaagg ctacctgacc aaacccgtgg acgatcgcac gctgatcagc acggtcaacg
     5341 aggctttgag cgcctaatcc ttgtcatgaa cggtcaagcg caaaacatac tcacggacat
     5401 ccgggccacc accatcgcca acgcggcgcc tcttccttcc cgggagaacg agcatctgga
     5461 gtggcagggt gtcggttatc agctgggcgg tgtccgcctg gtttctccaa tgggcgagat
     5521 cagcgagatt ctcgccgtgc cgaagttcgc gacgctgccc ggcgtcaaac cctggatgct
     5581 cggggtggcc aacgtccgcg gcaggctcat accgatcatc gacctccaca atttcctcga
     5641 catgcgcacg accacgccca tggttcagtg gcgcgtgctg gtagtggaag acgaggaact
     5701 gatcgcaggc ttcgtggtgg agcagtcgct cggcattcag cattttctag aagacagttt
     5761 tgaagaaagc acggcggagg aagtagcggc actgaagccc tacttgcgcg gtgcttttca
     5821 tcagggaggg tgcgtttttt acgaggcgca tcttaagtcg atattgaggg acgagaagtt
     5881 cttcgatgtt gcggacaaga ctaactaatc aggttgtacg gaaatgaaaa caggaaagtt
     5941 gtctccgttg ctagtggcgt tgatgtgctt gctggtcggc acgattgcag ggatggccta
     6001 cgttgtcgtc actgatgaag catccatcat caacaatacg ggtcttctgt tcgccgttgc
     6061 cggtatcgtg ctcgtcgccg ccatcgcggc gatgatggtc agccagacgc gccggcagct
     6121 cgttgagcag gcgtcacaga acgaacgcaa tcaggccgcg attcttcagc tgctcgacga
     6181 gatcgccgat ctcggtgagg gcgatctgag gattcaggcc tcggtgacgg agaacttcac
     6241 cggcgcgata gccgattcga tcaacttcgc catcgaccag atgagatcgg aagagcgtcg
     6301 t
//
LOCUS       DBNHKV010000095         6815 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_367627, whole genome shotgun sequence.
ACCESSION   DBNHKV010000095 DBNHKV010000000
VERSION     DBNHKV010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_367627"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="AC16PP_03115"
     CDS             complement(<1..468)
                     /locus_tag="AC16PP_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480643.1"
                     /translation="MSDRKTPGNPATSKDQSADRPFEERSTGDALSERRRSITAQKEE
                     AQHRLDQLEAEATAARRELSELAARKEHFKLLEQAVAGLEALSESGAADLFWGADAPS
                     SMLRVEDARARIARFSSEVEQVEAKHQKFVSDVAMQQQAQEDLEYDLFQIEQQE"
     gene            complement(490..1002)
                     /locus_tag="AC16PP_03120"
     CDS             complement(490..1002)
                     /locus_tag="AC16PP_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR6480644.1"
                     /translation="MKSSRRIKRMDKTRKRSKFVTKMNLTSLMDVFTILVFFLLVNSG
                     AADPLESPKDVKLPESRAESKPKETVVIYISPETVVVQGQPVAMVADILAASGEDVVA
                     IRDRLAAIKGQIIGPNTQQVAGSQEVTIMADKSIPFSVVKRIMSTCTSEGYGNISLAV
                     VQKDAQVAAI"
     gene            complement(999..1520)
                     /locus_tag="AC16PP_03125"
     CDS             complement(999..1520)
                     /locus_tag="AC16PP_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR6480645.1"
                     /translation="MARTHHYRRRVTEPTELDVTTFLNLMVVLVPFLLITAVFSRITI
                     VELDLPTSASSPSPNDPTFRVEVIVRDSGLEITNGRMVIAAIPKVEEEYDLQTLGGHM
                     LALKHDHPDIDEASVLLEPEIPYDYLIQVMDVVRSAEIQPEATQGNDGDGEVEQQPTQ
                     LALFTQIAIGDAP"
     gene            complement(1562..2224)
                     /locus_tag="AC16PP_03130"
     CDS             complement(1562..2224)
                     /locus_tag="AC16PP_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6480646.1"
                     /translation="MDFVYSIVEFFATGGAFMYPILLVFAVGAAIAIERFVTLTRVTT
                     KNQTAWSDIQPVLSQGDFERARKMTGQDQSPISRLLSMGLALQGAVRRREDVEIAMEE
                     GMMEIIPQLEKRTPYVALAASIATLLGLLGTIMGLIQAFTAVANANPAEKADLLSASI
                     SVAMNTTAFGLMVAIPLMVVHAFLSTKTNEIVDGMEMASVKVLTAISMRARRGEVTAK
                     AA"
     gene            complement(2362..2694)
                     /locus_tag="AC16PP_03135"
     CDS             complement(2362..2694)
                     /locus_tag="AC16PP_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480647.1"
                     /translation="MNRLLKLPLYFCLSALPAATSVAQEGDKAMSGMSILGNNDAPKA
                     LVIVPWKSSEIGDGIDVANLLDGRARAVDKVVFARELGYYELRVDAPEKSLSIREGAQ
                     MVNPVANQ"
     gene            complement(2700..3404)
                     /locus_tag="AC16PP_03140"
     CDS             complement(2700..3404)
                     /locus_tag="AC16PP_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6480648.1"
                     /translation="MYRPHSEPGPRIVLGGLILLAALLVGGCASTGPATRPALSKEAG
                     PTPTRERPARPARVKVEPNIGFTVTEVVALSSDVREMYHEALALLARNDLEAGIALLV
                     TVTERSPDATAPFIDLGIAHRRAGERDKAIASFTEALASTPDHPVAHNELGITYRELG
                     RFDEARRSYEQALTIYPGFHYARRNLAVLCDIYLADLACALEHYERYSQDIPDDREVE
                     IWVGDVRNRMPVTDEE"
     gene            complement(3385..6567)
                     /locus_tag="AC16PP_03145"
     CDS             complement(3385..6567)
                     /locus_tag="AC16PP_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6480649.1"
                     /translation="MPVVLAAMLLLALLAGCASRPDGGKTIEQLRGVKPDMTEVAIDD
                     SLNKALASYRHFLNETPVNNKTPDAMRRLADLQIEKEYGVIGDGSGVTLAAPARLAME
                     DAVGASTEGDAAIADHSEPEAEFERRATAPLALPSANWARAAEAGLPDGLSAAATAGP
                     LEAIATYQKILDRYPYYERNDQVLYQMARAYDELGEPDKAMEVMDWFINDYRYSHYAD
                     EVYFRRAEYFFVRKQYLEAEEAYSAVVSIGSGSQFYELALYKLGWTFYKQDFYEEALH
                     RFMALLDYKQSMGYDFDRVLAEQTSDPDGAGDEEAAEDGDLDGQTGAAVAAVDVPAAD
                     ATGEAEPDEDDDRRVADTFRVVSLSFSNLGGPQVINEYYATYGNRSYEDRIYSNLGEF
                     FLSKLRYNDAAKVYRSFTELHPFHRIAPHFSMRVVEIYAKGDFGQLVVEAKKDFARRY
                     GLNAEYWQHAEIEKSPDVLSYLKTNLKDLASHYHALYQMEELEKERPGNYAEALTWYR
                     ELLASFPTDDDSAATNYQLADLQFENGDFTEAAVEYERTAYDYPAHEKASAAGYAAVY
                     AHREHLKSIEAPPRSELRVAAVDSTMRFVDAFPAHDQAATVLGAATEDLYQMKRFEPA
                     ITAGRRLIESYPSAGEPLHLSAWTVVAHASFDLAVYPDAEQAYAEVLALTPEADEGRQ
                     ELFENLAASIYKQGEAASAMDDHRAAADHFLRVRTAAPTSAFRPAAEYDAASALMKLE
                     SWAEASAVFRDFRAAHEDHELQPEVTKQLAFVYRQAGELTRSAEEYERVAADALADGD
                     ETLRRESTLLAAELYEEAGSPKALAAFQHYVETFPEPMELAVEVRFKIAGMHKADGDE
                     AAYSGELRQIVAIDADAGAARTPRIRYLAAQSALVLTEERFTHFTELRLSQPFEKSLA
                     EKRKRMDAAMSAYEALVDYEVADVTAAATYYMAEMYGHFGESLLSSERPKDLNEEELA
                     EYELILEEEAFPFEESAIEVHEKNIELMVAGTFNTWIEQSLGQLSQLMPGRYAKSEIS
                     TGFVGPVEAYAYRTPVPIPAEETEDVSAAF"
     gene            complement(6567..>6815)
                     /locus_tag="AC16PP_03150"
     CDS             complement(6567..>6815)
                     /locus_tag="AC16PP_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480650.1"
                     /translation="RQAATLSYQGYDAPIKRLRGRIHQASAKVDQLMARQGRMLEQVA
                     MAELESRQRRLVNYQHQARYALADSYDRATQAQYAEEP"
BASE COUNT         1067 a         2164 c         2214 g         1370 t
ORIGIN      
        1 ttcctgctgc tcgatctgga agagatcgta ttcgagatcc tcctgagcct gttgctgcat
       61 cgcgacgtcg ctgacgaatt tctgatgctt ggcttcgacc tgctcgacct cggagctgaa
      121 tctggcgatg cgcgctctgg cgtcctcgac gcgcagcatc gaggatggcg cgtcggcccc
      181 ccagaagaga tcggcagcac cggattccga gagcgcttcc aggcccgcca ccgcctgctc
      241 gagaagcttg aaatgctctt tgcgggcggc aagctcgctc agctcgcgcc gggcggccgt
      301 ggcctcggcc tccagctggt cgagcctgtg ctgcgcctct tccttctgcg ccgtgattga
      361 tcggcgcctt tcgctgagcg cgtcgcccgt cgaccgttcc tcgaacgggc gatcagcgct
      421 ctgatccttc gaggtcgccg ggttgccggg tgttttacga tcagacatga ctaccttcgc
      481 tcatcccgat cagatggccg cgacctgggc gtccttctgc acgacggcga gagagatgtt
      541 cccgtatccc tcgctggtgc aggtggacat gattcgtttc accacgctga acggaatgga
      601 cttgtcggcc atgatcgtca cttcctggct gcccgctacc tgctgcgtgt tcggaccgat
      661 gatctggccc ttgatggccg ccagcctgtc cctgatagca acgacgtcct cgccggatgc
      721 tgcaagaatg tcggcaacca tggccacggg ctgtccctgc accaccacgg tttccgggct
      781 tatgtagatg accacggtct ccttgggctt ggactcggcg cgcgactccg gcagcttgac
      841 gtctttcgga gactccagcg ggtcggctgc gcccgagttc accagcagga agaaaaccag
      901 gatggtgaag acgtccatga gcgacgtcag gttcatcttc gttacgaact tggagcgctt
      961 tcgcgtcttg tccatacgct tgatgcgtcg ggagctcttc atggcgcgtc tccaatggcg
     1021 atctgggtga acagcgcgag ctgggtcggc tgctgctcga cttcgccgtc accgtcatta
     1081 ccttgggtag cttccggctg gatctcggcg ctgcgcacca cgtccatcac ctgaatcaga
     1141 tagtcgtagg gaatttccgg ttccagcaga acggaggcct cgtcgatgtc agggtgatcg
     1201 tgcttcagcg cgagcatgtg cccgccaagg gtctgaaggt catactcctc ttccaccttg
     1261 ggtatggccg caatgaccat ccgcccgttg gtgatctcca gcccgctgtc acgcacgatg
     1321 acctccaccc ggaaggtcgg atcgttcggc gacggcgagc tggccgaggt gggcagatcc
     1381 agctctacga tggtgatcct cgagaacacc gccgtgatga gcagaaacgg caccagcacg
     1441 accatcaggt tgagaaaggt ggtcacgtcg agctccgtag gctccgtcac gcgccgccga
     1501 tagtgatggg ttctggccat ctgtccctta gcctccgtct accccgttgg aatgcgtcgc
     1561 ttcaggctgc tttcgcggtt acctcgcccc gacgcgcgcg catcgagatg gcggtgagca
     1621 ctttgaccga cgccatttcc atgccgtcga cgatctcgtt cgtcttggtc gacaggaagg
     1681 cgtgcaccac catcaggggg atggcgacca tcagcccgaa cgccgtcgtg ttcatggcga
     1741 ccgagatgct ggcagacagc aggtcggctt tctccgcggg gttggcgttg gccactgccg
     1801 tgaatgcctg gatcaggccc atgatcgtgc ccagcagtcc gagcagcgtt gcgatgctcg
     1861 cggctagggc gacgtagggc gtgcgctttt ccagctgagg aatgatttcc atcatgccct
     1921 cttccatggc gatctcgacg tcctctcggc gtcgcaccgc accctgaaga gccagaccca
     1981 tggacagcag acgcgagatg gggctctggt cctggcccgt catcttgcgt gccctctcga
     2041 agtcaccctg ggacagcacc ggctgaatgt cgctccatgc cgtctggttc ttggtggtca
     2101 cccgggtcag cgtcacgaac cgctcgatgg cgatcgccgc gcctaccgca aagaccagca
     2161 ggatcgggta catgaaagca ccgccagtgg caaagaattc aacgattgag tacacgaaat
     2221 ccattgcgta aacctctccg caaaacgcct tgaatcagac gcaacttaaa gtgttgagat
     2281 gattgtgaac gtgatggggc gcacccttcg ccgttcggtt gttctgccag ttgatgagcc
     2341 tcacgtttcg acgccggccg ttcactggtt ggcgaccggg ttcaccattt gggcgccttc
     2401 ccgaatggaa agcgatttct cgggcgcgtc gacgcgaagc tcgtagtagc cgagctctct
     2461 tgcgaacacg actttgtcca ctgctcttgc ccggccgtcg agcaggttgg caacgtcgat
     2521 gccgtcgcct atctccgagc tcttccaggg cacgatgacc agcgccttgg gagcatcgtt
     2581 gttgccgagg atcgacatgc ccgacatggc tttgtcgcct tcctgggcca cagaggtcgc
     2641 cgcgggaagc gcgctcagac agaaatacag gggcagtttc aggagacggt tcatcttggt
     2701 cactcctcgt ccgtcacggg catccggttg cggacgtcgc cgacccagat ctcgacttcc
     2761 cgatcgtcgg gaatgtcctg gctgtatcgc tcgtagtgct cgagagcgca ggcgaggtcg
     2821 gccaggtaga tgtcgcagag cacggcgagg ttccgtcgcg cgtaatggaa gccgggatag
     2881 atggtcaggg cctgctcgta gctgcgtcgc gcctcgtcga agcggccgag ctcacggtag
     2941 gtgatgccca gctcgttatg ggccacagga tgatcgggtg tcgaggccag ggcctcggtg
     3001 aagctcgcga tcgccttgtc gcgctcgccc gccctgcgat gggcgatgcc gagatcgatg
     3061 aagggggcgg ttgcgtcggg agacctttcc gtgacggtga caagcagcgc gatgccggcc
     3121 tccagatcgt tccgcgccag cagggccagc gcctcgtggt acatctcccg gacgtcgctc
     3181 gacagggcga cgacctccgt cacggtgaaa ccgatgttgg gctccacttt cactcgtgcc
     3241 ggccgggcgg gacgttcccg ggtgggcgtc gggcctgcct ccttcgacag ggctggcctg
     3301 gtggcgggcc ccgtgctggc gcagccgccg accagcagcg cggccagcag gatcaggccg
     3361 cccagaacga tgcgggggcc cggctcagaa tgcggccgat acatcttctg tttcctctgc
     3421 tggtatcgga acgggcgtcc ggtaggcgta ggcttcgacg ggccccacga agccggtgct
     3481 gatttcgctc ttcgcgtagc gcccgggcat cagctgcgaa agctggccga ggctctgctc
     3541 gatccaggtg ttgaaagtcc ccgcaaccat gagctcgatg ttcttctcgt gcacctcgat
     3601 ggcgctctct tcgaacggaa acgcctcttc ttccaggatg agctcgtact ccgcgagctc
     3661 ctcctcgttg aggtccttgg gccgctcgga gctcagcagc gactcgccga agtggccgta
     3721 catctctgcc atgtagtagg ttgccgcggc ggtcacgtct gccacctcgt agtcgaccag
     3781 cgcctcgtag gcgctcatgg cggcgtccat gcgcttgcgc ttctccgcca ggctcttctc
     3841 gaacggctgg ctcagccgca gctcggtgaa gtgggtgaag cgctcctcgg tcagcaccag
     3901 cgccgactgc gcggcgagat aacgaatgcg tggcgtgcgt gcagcaccgg cgtccgcgtc
     3961 gatggcgacg atctggcgca gctcgcctga gtaggcggct tcgtcgccgt cggccttgtg
     4021 catgccggcg atcttgaagc ggacctccac cgcgagctcc atgggctcgg ggaaggtctc
     4081 gacgtagtgc tggaatgccg cgagtgcttt cgggctgccc gcctcctcgt agagctctgc
     4141 cgccagcagg gtggactccc ggcgcagcgt ctcgtcgccg tcggctagag catccgccgc
     4201 gacccgctcg tactcttcgg cggatcgggt cagctcgccg gcctgccgat agacgaaggc
     4261 cagctgcttg gtcacctcgg gctgaagctc gtgatcttcg tgcgccgcgc ggaagtccct
     4321 gaacacggcg cttgcctcgg cccagctctc cagcttcatc agggcgcttg ctgcgtcgta
     4381 ctctgcggcg ggtcggaacg ccgaggtggg cgctgcggtg cgcacgcgca ggaagtgatc
     4441 ggccgccgcc cggtgatcgt ccatggcgct ggctgcttcg ccctgcttgt agatggaggc
     4501 ggccaggttc tcgaacagct cctggcgccc ctcgtccgct tcaggcgtca aagccagcac
     4561 ctccgcgtag gcctgctcgg cgtcgggata gacggccagg tcgaacgaag cgtgcgcaac
     4621 caccgtccac gcggaaagat gcagcggttc gccggccgac gggtaggact cgatgagccg
     4681 cctgcccgcg gtgatggccg gctcgaagcg cttcatttgg tagaggtctt ccgtcgcggc
     4741 gccgagcacg gtggccgcct ggtcatgggc ggggaaggcg tcgacgaagc gcatggtgct
     4801 gtccacggcg gcgactcgca gctcgctgcg cggcggtgcc tcgatgctct tcaggtgctc
     4861 tcggtgggcg tagacggcag cgtagccggc ggccgaagcc ttctcgtgag ccgggtagtc
     4921 gtatgccgtg cgctcatact ccacggcggc ttcggtaaag tcgccgtttt cgaactgcag
     4981 atcggcaagc tggtagttgg tggccgcgga gtcgtcgtcg gtggggaagg atgccagcag
     5041 ctcccggtac caggtgagcg cctcggcgta gttcccgggc cgctcctttt cgagctcttc
     5101 catctggtag agggcgtgat agtggctggc gaggtccttg aggttcgtct tcaggtagct
     5161 caggacgtcg ggcgacttct cgatctccgc gtgctgccag tactcggcgt tgaggccgta
     5221 gcgacgggcg aagtctttct tggcttccac taccagctgg ccgaaatcgc ccttggcgta
     5281 aatctccacc acgcgcatgc tgaaatgggg cgcgatcctg tggaagggat gcagctcagt
     5341 gaaggaacga tagaccttgg ccgcgtcgtt gtagcgcagc ttgctcagga agaactcgcc
     5401 gaggttgctg tagatccggt cttcgtagct gcggttgccg taggtggcgt aatactcgtt
     5461 gatgacttgc ggtccgccca ggttggagaa ggacaggctc accacgcgga aggtgtccgc
     5521 cacgcggcgg tcatcgtcct cgtcgggctc ggcttccccg gtggcgtcgg cggccggcac
     5581 gtcgacggcc gcgaccgcgg cgcccgtctg cccgtcgaga tcgccatcct cggcggcctc
     5641 ttcatcgccg gccccgtcgg gatccgatgt ctgctcggcc agcacccggt cgaagtcgta
     5701 gcccatggac tgcttgtagt ccagaagcgc catgaaccgg tgcagggctt cctcgtagaa
     5761 gtcctgcttg tagaacgtcc agcccagctt gtacaaagcc agctcgtaga actgcgagcc
     5821 ggaaccgatg gagaccacgg ccgagtaggc ctcttcggcc tccagatact gcttcctcac
     5881 gaagaagtac tcggcgcggc ggaagtacac ctcgtcagcg tagtgagagt agcggtagtc
     5941 gttgatgaac cagtccatga cctccatggc cttgtccggc tcgccaagct cgtcgtaggc
     6001 ccgcgccatc tggtagagaa cctgatcgtt gcgctcgtag tacgggtagc ggtcgaggat
     6061 cttctgatag gtggcgattg cttccagcgg gcctgcggtg gctgctgcag acagcccgtc
     6121 gggcagacca gcctcggccg cacgcgccca gtttgcggac ggcagcgcca acggcgcggt
     6181 cgcgcggcgc tcgaattccg cttccggctc ggagtgatcg gcgatggcgg catcaccttc
     6241 ggtgctggcg cccaccgcat cctccatcgc cagacgcgcg ggtgcggcga gcgtcacgcc
     6301 gctgccgtcg ccgatgacgc cgtattcttt ctcgatctgc agatccgcaa ggcgccgcat
     6361 cgcgtccggc gtcttgttgt tgacgggcgt ctcgttgaga aagtgccggt agctggccag
     6421 cgccttgttg aggctgtcgt cgatggccac ctcggtcatg tcgggcttca cgccgcgcag
     6481 ctgctcgatg gtctttccgc catcggggcg gctcgcgcaa ccggcgagca gcgcaaggag
     6541 cagcatggcg gccagaacga cgggcatcac ggctcctccg cgtactgcgc ctgggtggcg
     6601 cggtcatagc tgtctgccag ggcgtatcgt gcctggtgct ggtagttcac caggcgccgc
     6661 tgccgtgact ccagctccgc catggcaacc tgctcgagca tccggccctg acgggccatg
     6721 agctggtcca cctttgcgga agcctgatga atccgcccgc gaaggcgctt gatgggcgcg
     6781 tcgtatccct ggtagctgag cgttgcggcc tggcg
//
LOCUS       DBNHKV010000096         2574 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1218946, whole genome shotgun sequence.
ACCESSION   DBNHKV010000096 DBNHKV010000000
VERSION     DBNHKV010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1218946"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="AC16PP_03155"
     CDS             <1..234
                     /locus_tag="AC16PP_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HIR6480651.1"
                     /translation="NMLVIHPDECIDCALCEPECPVDAIYSEDELPEDQQDFLEINRE
                     QAELWPNITEKKDPAEDYEDWIGKPDKRHLLER"
     gene            complement(251..1123)
                     /locus_tag="AC16PP_03160"
     CDS             complement(251..1123)
                     /locus_tag="AC16PP_03160"
                     /inference="COORDINATES: protein motif:HMM:NF015951.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HIR6480652.1"
                     /translation="MSARGARQWSGLFARGMAMGVAEVVPGVSGGTIAFITGIYHELV
                     TTLAGFRPASARLLVTAGPVAFWQQHNLGFLAVLVAGMAASVLLFARLIGYWLDVVPT
                     LVWAFFFGLIAASVFQIGRGRPTRTLAVYGTIGLAAGASLLLVDPLPAQGAVWVFFFA
                     GMIAISAWLLPAISGSFILLILGLYEPVIEALNGFDWVVLTSLGLGCATGLMIFARAL
                     EWLLSYWREPLLSLLTGFMAGSLVRLWPWAQGGTLLTPAEYAAVTGQASLELWAVAAA
                     AAGALALWLLSRLE"
     gene            complement(1134..1769)
                     /locus_tag="AC16PP_03165"
     CDS             complement(1134..1769)
                     /locus_tag="AC16PP_03165"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR6480653.1"
                     /translation="MTSQRTRDRLVQRLAEHGIVDQEILDLIGHVPRHIFVDEALSHR
                     AYEDSALPIGHGQTISQPFVVAFMTSTLLNAPRARVLEVGTGSGYQTAVLRSSRRVRR
                     VFTVERLGPLLARAEERLKAMGLESVRYRHGDGYEGWPEEAPFDGILVTAAPRQVPEA
                     LLDQLAEGGRMVVPVGGGGVQELRVYDRTATGVNVDTVRHVRFVPLVKGLL"
     gene            complement(1796..>2574)
                     /gene="truD"
                     /locus_tag="AC16PP_03170"
     CDS             complement(1796..>2574)
                     /gene="truD"
                     /locus_tag="AC16PP_03170"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HIR6480654.1"
                     /translation="RLAELYGVAAVDVGYAGMKDKRAVARQWLSVRTSLGPEVAERGG
                     VVVLAAGRDERKLRRGELAGNRFEIRLREVSGDDWRDRLGRVFESGAPNYFGPQRFGG
                     DNLDRALAWLPVRRRRSISPFRRGLYLSVLRSLLFNAVLARRVQEKTWRALLPGDVDL
                     DGMPTAPLWGRGRSATSGVALAAESAALAPFADVLDGLEHAGVDQARRSLVLLPQNAW
                     VAEEDEAVRLGFELPPGGYATSLLREAFELGEATDGAPAS"
BASE COUNT          510 a          914 c          813 g          337 t
ORIGIN      
        1 aacatgctcg tcatccatcc ggacgaatgc atcgactgcg ccctttgcga gcccgaatgc
       61 ccggtggatg ccatttactc ggaagacgag cttcccgagg atcagcagga cttcctggag
      121 atcaaccgcg agcaggccga gctgtggccg aacatcaccg agaagaagga tcctgcggaa
      181 gactacgagg actggatcgg caaacccgac aagcgccatc tcctcgaacg ctgaccgatt
      241 cggcctgcgt ctactccagc ctggacaaca gccagagggc caatgcgccc gcagcggccg
      301 cagcaaccgc ccacagttcc agactcgcct gacccgtcac cgcagcatac tcggccggcg
      361 tcagcagggt gccgccttgg gcccagggcc acaggcgcac cagcgagccc gccatgaatc
      421 ccgtcagtag agaaagcaac ggctcgcgcc agtaggagag cagccactcc agcgcccggg
      481 cgaagatcat aagcccggtg gcacatccga gacccagact cgtgagcacc acccagtcga
      541 acccgttcag cgcctcgatg acgggctcat agagcccgag aatcagcagg atgaagctac
      601 ccgagatagc ggggagcagc catgcagaga tggcgatcat cccggcaaag aagaagaccc
      661 aaacggcccc ttgggcgggc aacgggtcga cgagcaggag cgatgccccg gcagcgagcc
      721 cgatggtgcc gtacacggca agcgtacgcg tcgggcgacc gcggccgatc tggaacaccg
      781 aagcggcgat gagcccgaag aagaaagccc agaccagggt cggcaccacg tcgagccagt
      841 agccgatgag ccgggcgaag agcagaacgc tggcagccat acccgccacc agcacggcca
      901 gaaaccccag attgtgctgc tgccagaacg caaccggtcc tgcggtcacg agcagacgcg
      961 cggaagcggg cctgaacccg gccagggtgg tcacgagctc gtggtagatg cccgtgatga
     1021 aggcaatggt gcctccggac acaccgggaa ccacctcggc gacgcccatg gccatgccgc
     1081 gcgcgaagag accgctccac tgacgggcgc cgcgggcgct cacgctgcga ccctcatagc
     1141 aggcccttga ccagcggcac gaagcgtacg tgcctcaccg tgtccacgtt gacgccggtg
     1201 gcagtgcgat cgtaaacgcg cagttcctgc acgccaccgc cgcccacggg caccaccatc
     1261 ctcccgcctt ccgccagctg atccagcaga gcttccggaa cctgccgcgg tgctgccgtc
     1321 acgagaatgc cgtcgaacgg tgcttcctcc ggccatcctt cgtaaccgtc gccatggcgg
     1381 tacctgaccg attccagccc catggccttc aagcgctctt cggcgcgggc gagcagcggc
     1441 ccgagacgct cgacggtgaa gacccggcga acgcgccggc tcgatcgcag cacggccgtc
     1501 tggtatccgg acccggtccc tacttccaga acccgcgcac gtggcgcgtt cagcagcgtg
     1561 gaggtcatga aggcgacgac gaagggctgc gagatggtct gcccgtggcc gatgggcagg
     1621 gccgaatcct cgtacgcgcg gtgggacagc gcttcgtcca cgaagatgtg ccggggcacg
     1681 tggccgatca ggtcgagtat ttcctgatcg acgatgccgt gctcggccag tcgctgcacg
     1741 agccgatccc gggtgcgctg agaggtcatg ccgatgccga ccagatcgta gcccgtcacg
     1801 acgccggtgc tccatccgtg gcctcgccca gctcgaaagc ctcgcgcagc aggctggtcg
     1861 catatccgcc agggggcagt tcgaagccga ggcgcacagc ctcgtcttcc tccgccaccc
     1921 aggcgttctg cggcaacagc accagggatc gcctggcctg atccaccccc gcatgctcga
     1981 ggccgtctag cacatccgcg aacggggcga gcgcggcgct ctccgccgcc agggccacgc
     2041 cgctggtcgc agaccgaccc cggccccaga ggggggctgt gggcattccg tccagatcca
     2101 cgtcgcccgg gagcagtgcc cgccaggttt tctcctgcac ccgccgggcc agaaccgcgt
     2161 tgaagagcag gctgcggagc acggacaggt agagacccct cctgaacggc gagatggacc
     2221 ggcggcggcg tacgggaagc caggctaggg cgcgatcgag attgtcgcct ccaaaacgct
     2281 ggggcccgaa gtagttcggc gctcccgact cgaagacgcg cccgaggcgg tcgcgccagt
     2341 cgtcaccgga cacctcgcgc aaccggatct cgaaccggtt tccggcgagc tcaccccgac
     2401 gaagcttgcg ctcatcacga cctgcggcaa gcacgaccac gccgccccgt tccgccacct
     2461 cggggccgag agacgtgcgc acgctcaacc actgacgggc aacggcgcgc ttgtccttca
     2521 ttccggcata gcccacatcc acggccgcga ccccgtaaag ctcggccaga cgac
//
LOCUS       DBNHKV010000097         5061 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_438847, whole genome shotgun sequence.
ACCESSION   DBNHKV010000097 DBNHKV010000000
VERSION     DBNHKV010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_438847"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="AC16PP_03175"
     CDS             <1..337
                     /locus_tag="AC16PP_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480655.1"
                     /translation="DVHALGDLTPRTVFYQPPRKQADGVLTASHRFDQAGGYIGIVTA
                     SHPTQEKIYNAVFFFRVGGPDLGYLPLFVGLIILVQLIYWGATGTLQRFLRRYLPNRR
                     NYGEAAAAG"
     gene            920..1234
                     /locus_tag="AC16PP_03180"
     CDS             920..1234
                     /locus_tag="AC16PP_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480656.1"
                     /translation="MNHPVTQRSLRQQNQAFSGTNGVSQNNRSMRFLPAFRDEETGQV
                     ELARFDDGRVAPAHLIVGLPRRWAVAFASDGAILALKPSITAGFVRDDVFFTREEAAS
                     AD"
     gene            complement(1231..2451)
                     /locus_tag="AC16PP_03185"
     CDS             complement(1231..2451)
                     /locus_tag="AC16PP_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6480657.1"
                     /translation="MAAPLEDITVVAIESWMAAPSASALLADLGANVIKVEPPSGDPM
                     RNLSRAPKVDGPLKALDCQFDVDNRGKRSVAVDLDTDQGKAVVHRLVARADVFMCNLL
                     IHRQERFGLDPDSVLALNPRIVHATLTGYGTTGPDAWRPGYDVTAFFGRSGLYDASRE
                     GDDGDVPMARPAQGDHTTGLALLGAILAALRSAERTGEGQVVETSLFETAIWTQASDF
                     GTTAIDRAPVRRRARHNMLSPTANRYPCGDGKWLVVNMLDAMAFDRFCRAIGREDWLE
                     DERFREARGRYDHMPELVTGIDATLATRSRDEWGRIFDEAGLIWGPVLTLDEAAGDGQ
                     AHAIGMFPELEHPEYGTYRTVNAPFRFRSGEVRPRGTSPGPGEHTREVLGEMDFDEAE
                     IDALMAAGVLPTHD"
     gene            2574..3092
                     /locus_tag="AC16PP_03190"
     CDS             2574..3092
                     /locus_tag="AC16PP_03190"
                     /inference="COORDINATES: protein motif:HMM:NF021438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HIR6480658.1"
                     /translation="MRELHYLRGPWAAAASILMLVTGVLWTPVASANQTVDTDAKASR
                     VEGVWASKGAVFAIERSGDTLQATVVALRKPRPDRKNAKAELRDRPLIGLQLLSDYRF
                     KRGRWRGDFYDPGSGRTYSSYIRLDDEGNLRLRAWVLMFGHTEVMEPVGNCTERIIAM
                     LEVSGVDGLCPP"
     gene            3112..4248
                     /locus_tag="AC16PP_03195"
     CDS             3112..4248
                     /locus_tag="AC16PP_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017845642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HIR6480659.1"
                     /translation="MSVVPVSGRSGLNDFIEVPWVVYADDPCWVPPLKMERRDALSPK
                     HPFFEHARWQPFVAYRDGRAVGRISAQIDELYQQRHDPKGGFFGMFESIPDDAVIDGL
                     LASAEAWLAERGCEHVVGPFNLNINQEVGLLVDGFDSSPFFMMGHGMPYYGEALARRG
                     YAEVQRMLAYEMPVLFERPRLMSIMMKQLGPRVTMRTLDRRKLVEELEILRDIFNDAW
                     SENWKFVPWTSREFLAVGKEMMMLIPPDLVQIAEVDGEPAAFIVMLPNLNEVIGDLDG
                     RLLPFGWAKLLWRIKVRYPKSGRVPLMGVRRRFHRTRLGPGLAMFCISALREPAHRAG
                     LERAELSWILEDNQGMRSILEAVGGEVTRTYAMFEKSLSRGPGS"
     gene            4245..5036
                     /locus_tag="AC16PP_03200"
     CDS             4245..5036
                     /locus_tag="AC16PP_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HIR6480660.1"
                     /translation="MSEVSVCGAVVLAGQRAGPTPLCAQAGVDVEVLVPVGGVPAIER
                     VLGALSRAELVRPAVVVGPSPEVLSAHPEVKAMIDAAGAAWVAPAGDPASSALAGLQG
                     QAPPTLVTSGDHALLCASIVDAFCEAAHRVQADLIVGLVPYQAVRAAYPESRRTVLKF
                     ADGARCGANLYFLRTEEARRGIEFWVAVQRDRKRPWRIARRLGWRLLLRYLTGRLTLG
                     AALGALSERAGCRLGSVIVDEPRAAVDVDSVDDWRLAQRIVGEAS"
BASE COUNT          801 a         1627 c         1713 g          920 t
ORIGIN      
        1 tgacgtgcat gcccttggcg atctgacgcc gcgcacggtc ttctaccagc cgccccggaa
       61 acaggccgac ggggtgctga ccgccagtca tcggttcgac caggcgggcg gctacatcgg
      121 catcgtcacg gcaagccacc cgacgcagga gaagatctac aacgcggtgt tcttcttccg
      181 cgtgggtggc ccggatctgg gctaccttcc cctcttcgtc ggtttgatca ttctcgttca
      241 gctcatctac tggggggcaa ccggcacttt gcagcggttt ctccgccgct accttcccaa
      301 tcggaggaac tacggggaag cggcggcggc cggttaggaa tccggcgtcg tgcccgggac
      361 ggcctgccag tcaaggggaa gctggggaga tcgggcggaa acgtacgtcg tcggctagaa
      421 gccggtttcc accatgtcgc gcaagcatgt cgcgcaaggc atcttcgcgg gtcgcgaagc
      481 agccatgatc gatctccatc ttcggaggtc ggcccaccgt atctgctgtt cagacccacg
      541 ccgcatgtgg cgcggaacga tgactttagc ccgttgcccc gaatgcgcga gctggcgtcg
      601 gatggaactg gttcgggccg ttcatgtgaa ctcgagcagg cggcacgcca tcgcgccccg
      661 ggcgcggtgg ggcagccggg cgcgcaactg acaagcggcg tcacacgaaa gtgtcctgct
      721 acggcgtttc gttcacgatt tgttcatgga aaacagggtg ttatggagcc tcggctgaaa
      781 ttctggagtt tggcatggtt gatgctaaac gaaacggtag gtaacgaatg agcgaatctg
      841 aaacgcaact gccattccag ctgaggtctc gactggcatg tgcggagctt cagggaagtt
      901 gtaggagctt cacgtcgtca tgaaccatcc ggtcacccaa aggtcgctgc gccagcagaa
      961 ccaggccttc tccggcacca acggtgtcag ccagaacaac cgctcgatgc gtttcctccc
     1021 ggccttccgc gacgaggaaa ccggacaggt ggagctggcg cgcttcgacg acggcagggt
     1081 cgcgcccgcg cacctcatcg tcgggctccc tcgtcgatgg gcggtcgcat ttgcgagcga
     1141 cggtgccatt ctggcgctca aaccgagcat caccgccggg ttcgtccgcg acgacgtctt
     1201 cttcacccgc gaggaagccg cctctgcgga ctagtcgtgc gtcggcaaca ccccggccgc
     1261 catcaacgcg tcgatctccg cctcgtcgaa atccatctcg ccgagcactt ctctggtgtg
     1321 ctcaccggga cccggtgacg ttcctctcgg cctgacctcc cccgatcgga atcggaacgg
     1381 cgcgttgacc gtccggtagg tgccgtactc cgggtgctcg agctcgggga acatgccgat
     1441 ggcgtgagcc tggccgtcgc cggcggcctc gtcgagggtc agcaccggcc cccagatcag
     1501 gccggcttcg tcgaagatcc ggccccactc atcgcggctg cgggtcgcca aggtcgcatc
     1561 tatgcccgta accagctcgg gcatgtggtc gtagcggccg cgggcttcac ggaaacgctc
     1621 gtcctccagc cagtcttctc tgccgattgc ccggcagaag cggtcgaacg ccatggcatc
     1681 cagcatgttc accaccagcc acttgccgtc gccgcacgga tagcggtttg cggtcgggga
     1741 gagcatgttg tgacgcgcac gccgacgcac gggggcacgg tcgatggccg tggtgccgaa
     1801 atccgaggcc tgggtccaga tggccgtctc gaacagagaa gtttccacca cctggccctc
     1861 gcccgtgcgc tcggccgatc gcaacgcggc gaggatggcc ccgagcagcg ccaggccggt
     1921 ggtgtggtcg ccctgggccg ggcgcgccat gggcacgtct ccgtcgtcgc cctcgcggct
     1981 ggcatcgtag agacccgagc ggccgaagaa ggcggtcacg tcgtagccgg ggcgccaggc
     2041 gtctggcccg gtggtgccgt agccggtcag cgtggcgtgc acgatgcgcg ggttcagcgc
     2101 caggacgctg tcgggatcga gcccgaaacg ttcctgccga tgaatgagca ggttgcacat
     2161 gaacacgtcg gcccgggcga cgaggcgatg cacgacggcc ttgccctggt cggtgtccag
     2221 gtccacggcc accgatcgct tgccgcggtt gtccacgtcg aactgacagt ccagcgcttt
     2281 gagcggaccg tcgaccttgg gcgcgcgcga gagattgcgc atgggatcgc cgctcggcgg
     2341 ctcgaccttg atcacgtttg cgccgaggtc tgccagcagg gcgctcgcgc tcggcgccgc
     2401 catccagctc tcgatggcga cgacggtgat atcttccagc ggtgcagcca acggctcgcc
     2461 tcctctttga gctcacgggg cgtgaaagcc cggttgtacc gcatattcgc ggcaaattca
     2521 gcttccgcgc gagaactggc agtagcttcg gcggcgcggt aaccttgcgc gccatgcggg
     2581 aactgcacta tctgcgaggg ccgtgggcgg cggctgcttc gatcctcatg ctcgtgacgg
     2641 gcgtgctctg gacacccgtc gcctcggcca accaaaccgt agacacggac gccaaagcca
     2701 gccgcgtaga gggggtctgg gcctccaagg gtgccgtgtt tgccatcgag cgctccgggg
     2761 acaccctgca agccaccgtc gtcgctctgc gcaaaccccg acccgaccgc aaaaacgcta
     2821 aagccgagct gcgcgaccgg ccgctgatcg ggctgcagct gttgagcgac tatcgtttca
     2881 agaggggccg ctggcgcggg gatttctacg acccggggtc aggcaggacc tattccagtt
     2941 acatccggct ggatgatgag ggcaacctta ggcttcgcgc ctgggtgctg atgttcggtc
     3001 acaccgaagt gatggagccg gtcggcaact gcaccgaaag gatcatcgcc atgctcgaag
     3061 tgtcgggggt cgacggcttg tgtcccccat aacgttcgct tcggcatgtc attgagcgtc
     3121 gtacccgtgt ccggacggtc cgggcttaac gacttcatcg aggtgccctg ggtcgtgtat
     3181 gcggatgacc cgtgctgggt tccgccgctg aagatggagc gccgggacgc gttgtccccc
     3241 aagcatccct tcttcgagca cgcccgctgg cagccgttcg tcgcgtaccg cgacggccgg
     3301 gccgtcggcc gcatctccgc ccagatcgac gagctgtatc agcaacgcca cgaccccaag
     3361 ggcgggttct tcggcatgtt cgaaagcatc ccggacgacg ccgtcatcga cggcctgctc
     3421 gcaagcgccg aggcgtggct ggccgagcgt ggctgcgagc acgtggtggg tccgttcaac
     3481 ctgaacatca accaggaggt gggcctgctc gttgacggct tcgattcgtc gcccttcttc
     3541 atgatggggc atggaatgcc ctattacggc gaagcgctgg cgcggcgtgg ttacgccgag
     3601 gtgcagcgca tgcttgccta cgagatgccc gtgctcttcg aaaggccgcg gctgatgagc
     3661 atcatgatga agcagttggg cccgcgggtg acaatgcgca cgctcgaccg gcgcaagctc
     3721 gtcgaagagc tcgaaatact gcgcgacatc ttcaatgatg cctggtccga gaactggaag
     3781 ttcgtcccct ggacgtccag agagtttctt gccgtgggta aggaaatgat gatgctgatc
     3841 ccgccggatc tcgtgcagat agccgaggtc gacggcgagc cggcagcgtt catcgtcatg
     3901 ctgccgaacc tcaacgaagt catcggtgat ctcgacggcc ggctgcttcc gttcggctgg
     3961 gccaagctgc tctggcgcat caaggtccgc tatccgaagt ccggccgggt gccgctcatg
     4021 ggcgttcgcc ggcgctttca ccgcacgcgg ctcggtccgg gcctggccat gttctgcatc
     4081 agcgccctgc gtgagcccgc ccatcgtgcc ggtctcgagc gggcggaact ttcttggatc
     4141 ctggaagaca atcagggcat gcgcagcatt ctcgaggcgg tcggggggga agtcacccgg
     4201 acctatgcca tgttcgagaa gtcgctttct cgaggccccg ggtcgtgagc gaggtgagtg
     4261 tttgcggcgc ggtggtgctc gccggccagc gtgccggccc gacgcctttg tgcgcccagg
     4321 cgggcgtcga cgtagaagtt ctcgttccgg tgggcggcgt gccggccatc gagcgcgttc
     4381 tgggggccct ttcgcgcgcg gaactcgtgc gcccggctgt ggtggtgggc ccgtcccccg
     4441 aggtgctgtc ggcccatcct gaggttaagg caatgatcga cgctgccggg gcggcctggg
     4501 ttgctccggc cggcgacccg gcctccagcg cacttgccgg cctgcaaggc caggcaccgc
     4561 cgacgctggt caccagcggc gatcacgcgt tgctctgcgc atccatcgtc gacgctttct
     4621 gcgaggctgc gcaccgggtg caggcggacc tgatcgtcgg cctggttccc taccaggcgg
     4681 tgcgggccgc ctatccggaa agccgcagaa cggtgctcaa gttcgccgac ggtgccagat
     4741 gcggcgccaa cctctatttt ctgcgcaccg aggaggcgcg gcggggcatc gagttctggg
     4801 tggccgtgca gagggatcgc aagcgtccct ggcgcatcgc gcgtcggctc ggatggcgcc
     4861 tgctgctgcg ctacctgacg ggacggctga ctctgggggc cgcgctcggg gcgttgtcgg
     4921 agcgcgccgg ctgccggttg ggatcggtga tcgtggacga gccgcgcgcc gcggtggatg
     4981 tggacagcgt cgacgactgg cggctcgcgc agcggatcgt cggagaggcg tcgtgagcgg
     5041 gaccgcttcg cttcccaggc g
//
LOCUS       DBNHKV010000098         4873 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_723107, whole genome shotgun sequence.
ACCESSION   DBNHKV010000098 DBNHKV010000000
VERSION     DBNHKV010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_723107"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="AC16PP_03205"
     CDS             complement(<1..523)
                     /locus_tag="AC16PP_03205"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase family protein"
                     /protein_id="HIR6480661.1"
                     /translation="MKPQPFRIEVPEATLRDLADRLDRSGWPDDFANDDWSYGTNGAY
                     LRDLVGFWRRDYDWRAVERKINAFDHYRVEIEGMPVHFLREPGRGPNPVPLILSHGWP
                     WTFWDLNEVIRPLADPASFGGDPEDAFEVIVPSLPGFGFSTPLTTPGINFWRTADLWH
                     TLMTEVLGFQRFGA"
     gene            complement(530..1093)
                     /locus_tag="AC16PP_03210"
     CDS             complement(530..1093)
                     /locus_tag="AC16PP_03210"
                     /inference="COORDINATES: protein motif:HMM:NF025050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6480662.1"
                     /translation="MSNGTMPIFQHAYFVNDIEEAAHRWNRLYGAGPFVLVPHHKTDK
                     FQYRGTDQEADVSYGFGYLGENQIQFIQQHDETPSIYRDMYQAGEEGFHHVGCLVNDF
                     AAARQRMLDLGFVNACELWADDVNAAYFDTRSVNGGFTEIHGDPVHILGTFATWKRAH
                     QNLKPGDSPIMDMSAPFPDGRDPGYLD"
     gene            complement(1136..2173)
                     /locus_tag="AC16PP_03215"
     CDS             complement(1136..2173)
                     /locus_tag="AC16PP_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6480663.1"
                     /translation="MKLGILGGNIGRKTGINIEAIRKAESLGYDSVWTAEAWGGDAVT
                     PATWILAQTEKIKVGTAIMQMPARTPAMAAMTAMSLAELSGGRFIIGLGASGPQVVEG
                     WHGVPYGKPMTRLREYVAIMRKIFAREDKLTFDGEMYQIPYTGPGSTGLGKPLKSILH
                     CEEDIRIFAASITPKGVETAAEVADGFFPIWMDPEKFSVFEEPIRKGFAAAGDKDLSS
                     FEVSPFVTVVIGDDVEQCMAPIRGNMALYIGGMGARNKNFYNDYAKALGFEDAAVEIQ
                     DHFLAGRREEAMRAVPAELIDACHLVGPADRIRERLQRWKAAGGNGQVASMLLGTQQP
                     EALELVASEML"
     gene            complement(2208..3884)
                     /locus_tag="AC16PP_03220"
     CDS             complement(2208..3884)
                     /locus_tag="AC16PP_03220"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6480664.1"
                     /translation="MLSRSEALEALTAPGQPYELVDVELYGRSCRAFRNAPPLLRDLY
                     ASTASDLPFIVYGGERLSFADAWAESCRIAILLMEQYGVAKGDRVAISMRNYPEWMTA
                     FAAVTSIGAIAVAMNALWTPEEMEYGLKDSGAKVLLADEERLARLALCADPPAGLAVL
                     AVRAADDAGTGARDLREALAGVPAGNMPAVDVAPEDDATILYTSGSTGHPKGAVSCHR
                     SLVTALLSWELDAHAGAYMQGVDLPAPESQPATLLAVPLFHATGSHAVYLASYRSQRR
                     VVSMYKWDVEEAAELIERERISSFVAPPAMTGDLVQFAARTNRDLSCLDRVGGGGAAR
                     APEQVRGIRRTFAKAMPSTGWGMTETNAIGTVISGQEYLDRPESSGRCSAVLELRVVD
                     PEGRELPCGERGELQVRGTSLIRGYWNRPEATAEAIVEGWLRTGDVAYLDDDGYLFIV
                     DRIKDLVIRGGENIGCGAVEAALQEHPDVIEACVYAVPDERLGEEVGATVYICRPVDE
                     EEIRNFLAPRLARFEIPRYLSFSTQPLPRTASGKIFKRQLKQEAADRLTG"
     gene            4022..4810
                     /locus_tag="AC16PP_03225"
     CDS             4022..4810
                     /locus_tag="AC16PP_03225"
                     /inference="COORDINATES: protein motif:HMM:NF023358.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 domain-containing protein"
                     /protein_id="HIR6480665.1"
                     /translation="MKPYGKRHASWVWLTLFLLTPNPSRSEAPTATAGDALDQAQSYQ
                     TIAGRSQARVEDLDDAALAMLSQYNGEVDRYEDLRTYNDNLRELLASQRAEKERLNAE
                     LAGIEIVRQEIVPLMVQMIDVLDSFVALDQPLLIDERTSRVESLRRNLTRSDVDLAER
                     YRRIIEAYQIEAEYGQTIEAYEGPIVIDDRELTVDFLRVGRIGLYYLSLDRAEGGIWN
                     PAEKRWTPLAENYLDDLDLAVRMARKQAPPNLMDLPMWTTGGES"
BASE COUNT          866 a         1613 c         1545 g          849 t
ORIGIN      
        1 gggcgccgaa gcgctgaaag ccgagcacct cggtcatcag cgtgtgccag agatcggccg
       61 tcctccagaa gttgatccca ggggtcgtga gcggggtcga gaagccgaag ccgggcagcg
      121 atggcacgat gacctcgaac gcatcctcgg gatctccgcc aaacgacgcc ggatcggcca
      181 gggggcggat cacctcgttg aggtcccaga acgtccaggg ccagccgtgg ctgagtatca
      241 ggggcaccgg gttcgggccg cgccccggct cgcgcagaaa atgcacgggc atgccctcga
      301 tctctacccg gtagtgatcg aaagcattga tcttgcgctc caccgcgcgc cagtcgtagt
      361 cccgtcgcca gaacccgacc aggtcccgca ggtaggctcc gttggttccg taggaccagt
      421 catcgttcgc gaagtcgtcg ggccagccgg aacgatcgag ccggtcggcc agatcgcgca
      481 gcgtcgcttc cgggacctcg atgcggaaag gttggggctt cacggcgctt cagtccagat
      541 acccgggatc cctgccgtcc gggaacggtg ccgacatgtc catgatgggg ctgtcgccgg
      601 gcttcaggtt ctgatgcgcc cgcttccagg tcgcaaaagt acccaggatg tgcaccggat
      661 ctccgtggat ctcggtgaag ccgccgttca ccgagcgcgt atcgaaatac gcggcgttaa
      721 cgtcgtccgc ccagagctcg caggcgttca cgaaacccag atccagcatg cgctgacggg
      781 cggcagcgaa gtcgttgaca agacagccga cgtgatggaa gccctcttcc cccgcctgat
      841 acatgtcccg atagatcgac ggggtttcgt cgtgctgctg gatgaactgg atctggtttt
      901 cgcccaggta gccgaacccg taggatacgt ccgcttcctg gtccgtgccc cgatactgga
      961 acttgtccgt tttgtggtgg ggcacgagca cgaaaggccc ggctccgtag agccggttcc
     1021 accggtgagc ggcttcctcg atgtcgttga cgaagtacgc gtgctgaaag atcggcatgg
     1081 tgccgttact cattgctgtt gtctcctttt cctgtgaagg tcgtcctgag cgcccctaga
     1141 gcatctccga ggcgaccagt tccagcgctt cgggctgctg ggtgccgagc agcatgctcg
     1201 ccacctgacc gttgccgccc gccgccttcc atctctgcag cctttcccga atgcggtcgg
     1261 ccggacccac caggtggcag gcgtcgatca gctccgccgg caccgcgcgc atggcctctt
     1321 cgcgccgtcc ggccaggaaa tgatcctgga tctcgacggc ggcgtcttcg aacccgagcg
     1381 ctttggcgta gtcgttgtag aagttcttgt tgcgggctcc catcccgccg atgtagagcg
     1441 ccatgtttcc acggatgggc gccatgcact gctccacgtc gtccccgatg accacggtga
     1501 cgaacggcga cacctcgaaa ctgctcagat ctttgtcgcc agctgcggca aaacctttcc
     1561 gtatgggctc ctcgaacacg gagaactttt ccggatccat ccagatcggg aagaacccgt
     1621 cggcaacctc cgcggcagtc tccacaccct tcggcgtgat gctcgcggcg aagattctga
     1681 tgtcctcctc gcagtgcagg atgctcttca gcggtttgcc caggcccgtg gagccggggc
     1741 ccgtgtaagg gatctggtac atctcgccat cgaaggtgag cttgtcctca cgggcgaaaa
     1801 tcttgcgcat gatggccacg tactctctca gccgcgtcat gggcttgccg tacggcacgc
     1861 cgtgccagcc ttccactacc tggggccccg aagcaccgag gccgatgatg aaccggccac
     1921 cggagagctc cgccaggctc atggccgtca tggcggccat ggccggcgtc cgagcaggca
     1981 tctgcatgat ggcggtgccc accttgatct tctcggtctg cgcgagaatc caggtggcgg
     2041 gcgtgaccgc gtcgcctccc caggcttcgg ccgtccagac cgagtcgtag ccaagggact
     2101 cggccttgcg aatcgcctcg atgttgatgc ccgtcttgcg gccgatgttg ccgccgagaa
     2161 tgccgagttt catgtgatat gcctcctttg cgagtaatgc gatgagttca gccagtgagc
     2221 cggtccgctg cctcctgctt gagttggcgc ttgaagatct tgcccgatgc ggtgcgcggc
     2281 agcggctggg tggaaaaaga cagatagcgg gggatctcga agcgtgccag gcggggagcc
     2341 agaaagttcc tgatctcctc ctcgtcgacc gggcggcaga tgtagacggt cgcgccgact
     2401 tcctccccca ggcgctcgtc gggcaccgcg taaacgcacg cctctatcac gtccgggtgt
     2461 tcctgcaacg ccgcctccac ggcgccgcag ccgatgttct caccgccccg tatgaccagg
     2521 tccttgatac ggtcgacgat gaagaggtaa ccgtcgtcat ccagataggc cacatccccg
     2581 gtgcgaagcc acccttcgac gatcgcctcc gcagtggcct ccgggcgatt ccagtaaccg
     2641 cggatgagcg aggtgccgcg aacctgcagc tcgccgcgct ctccgcaggg cagctcgcga
     2701 ccctcgggat ctaccacccg caattccagc acggcgctgc agcggcccga gctttccggg
     2761 cgatccaggt attcctgacc ggagatgaca gtgccgatgg cgttggtctc cgtcatgccc
     2821 cagcccgtgc tcggcatggc cttggcaaag gtgcgtcgga tcccgcgcac ctgctcgggc
     2881 gcacgggccg caccgccgcc gccgacccga tccaggcagc tcagatcccg gttggtgcgt
     2941 gcggcaaact ggacgagatc ccccgtcatg gccggcggag cgacgaaaga cgagatgcgc
     3001 tcgcgctcga tgagctcggc agcctcctcg acgtcccact tgtacatgga aacgacccgc
     3061 cgctgagacc ggtaggaggc aagatagacc gcatgggagc ccgtcgcgtg aaacagcggc
     3121 acggcgagca gcgtggccgg ctgagactcg ggagccggca gatcgacgcc ctgcatgtag
     3181 gcgcccgcgt gcgcgtcgag ttcccaggaa aggagcgcgg tgacaaggct gcggtgacag
     3241 gagaccgcgc ctttggggtg tcccgtggag ccggaggtat acaaaatggt ggcatcgtcc
     3301 tccggggcga cgtccaccgc cggcatgttg cctgccggga ccccggcgag ggcctctcgc
     3361 agatcccggg cgccggtccc cgcgtcatcg gcggcccgca ccgccagaac ggctagaccc
     3421 gccggcggat cggcacaaag cgccaatcgg gcaaggcgtt cttcgtctgc caggaggacc
     3481 ttggcgccgc tgtccttcag gccgtactcc atttcctccg gcgtccagag cgcgttcatg
     3541 gccaccgcga tggcgccgat ggacgtgacc gcggcaaagg ccgtcatcca ctccgggtag
     3601 ttgcgcatgg agatggcgac cctatcgccc ttggcgaccc cgtactgttc catcagcagg
     3661 atggcgattc ggcaggactc tgcccaggcg tccgcaaagg aaaggcgctc gccgccatag
     3721 acgatgaacg gcaggtcgct ggcggtggac gcgtatagat cccgaagaag gggcggcgcg
     3781 ttgcgaaagg cacggcagct gcgaccgtac agctcgacgt ccacgagctc gtagggttga
     3841 cccggcgcgg tcagcgcctc caaggcctcg gatctgctca acatgtgcct tccacctctc
     3901 ccgctgcaaa cgatacccgc gctcgctatg cgccgacaac tcgccattac ggggattcac
     3961 ggataaccaa agcattcgcg ccctatatcc tcaaaatcgt gtaaacgtgg gaggggtcct
     4021 cgtgaagcca tacggcaaac gacacgcgag ctgggtctgg ctgacccttt ttctgctgac
     4081 gccgaatccg tcgcggagcg aggcgccgac cgctaccgca ggcgacgctc tggaccaggc
     4141 gcagtcctat cagaccattg caggccgcag ccaggcccgg gtcgaggatc tcgacgacgc
     4201 ggccctggcc atgctctccc agtacaacgg cgaggtcgat cgctacgagg accttcgcac
     4261 gtacaacgac aacctgcggg agctgctggc cagccagcgc gccgagaagg aacgactcaa
     4321 tgccgagctt gccgggatag agatcgtccg tcaggagatc gtccccctaa tggtgcagat
     4381 gatcgacgtc ctcgacagct tcgtcgcgct ggaccagccg ctcctgatcg acgagcgcac
     4441 gtcccgggtg gagtcgctgc gaaggaacct cacgcgttcc gacgtagacc tggccgagcg
     4501 ctaccgccgt atcatcgagg cgtatcagat cgaggcggaa tacgggcaga ccatcgaggc
     4561 gtacgaagga cccatcgtca tcgacgaccg ggaactgacc gtcgactttc ttcgggtcgg
     4621 ccgcatcggg ttgtactacc tttccctgga ccgggccgag ggcggcatct ggaacccagc
     4681 cgagaagcgc tggacgcccc tggcggaaaa ttatctggac gatctcgacc tcgccgtccg
     4741 gatggcacgc aagcaggctc cgccaaacct gatggacctt cccatgtgga cgacgggagg
     4801 cgaatcatga ggcggacggc gatcacgctg ctggccctgc tcgcgcccgc gagctgggcc
     4861 gcagccccca cca
//
LOCUS       DBNHKV010000099         1743 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1077227, whole genome shotgun sequence.
ACCESSION   DBNHKV010000099 DBNHKV010000000
VERSION     DBNHKV010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1077227"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1668)
                     /locus_tag="AC16PP_03230"
     CDS             complement(166..1668)
                     /locus_tag="AC16PP_03230"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR6480666.1"
                     /translation="MKQKWYLLALVSAALVCQIAQAGVDTVRVSEAFREASVAANSDR
                     ELAPDQPPDQRLAAGMQLTAHVARSHTRDFIRRPLTSVGNLLAATFTGATGIVKELAL
                     DFVLVPRLASKAARPLSSSRGMDIEEWENDLDRITGTERTRGQMKLLVDGEAFFDRLI
                     AEIAAARRSIEIRTYIFDNDDYAVAIADLLRRRSREVAVTVMADGLGTIGGTLATADS
                     QPADYVMPVSMGAYLKEGSGVRLQMLGNTWFMGDHTKTYIFDERVAFLGGMNIGREYR
                     YDWHDIMVELTGPVVGELSSEAAQAAREDRLGDLALLGSTKRTPRTDGAEGGQELRLL
                     RTRPHDAQIYRAQLEAIRRAEKHIFVQNAYLGDDQIIYELAKARRRGVDVRVILPARP
                     DSELVRRSNALAINTLRRFGVRVYLYPGMTHVKAAIYDGWACFGTANFDRLSLRINKE
                     VNIATSDPAFVGSLRAQVFDVDFARSREVVDDEPTGASDHLYERIVDILL"
BASE COUNT          319 a          583 c          529 g          312 t
ORIGIN      
        1 ccatcctggc gcagcgtcgt cagaaccccc cagcgatgat cggtgatgaa ctgatcctga
       61 tcggcgttct cggtaatcaa ggcgaccctc cacaagcatt cctgtccaga ccatcgacga
      121 tagcccaaca ccgttcagcg accaagggtc ttgcgcccgc agcgtctaca ggaggatgtc
      181 cacgatgcgc tcgtagagat ggtcggacgc tccggtgggc tcgtcgtcca caacctcgcg
      241 cgaccgggcg aagtccacgt cgaacacctg ggcgcgaagc gacccgacga acgcgggatc
      301 ggacgttgcg atgttcacct ctttgttgat gcgcaggctg agccggtcga agttggcggt
      361 tccaaagcac gcccagccgt catagatggc cgctttcacg tgggtcatcc cgggatagag
      421 gtagacgcga acgccgaagc gtcgcaatgt gttgatcgcc agggcattgc tgcgccgaac
      481 cagctcgctg tcgggccgcg ccggcaggat cacccgcacg tcgaccccgc gtcgccgcgc
      541 tttcgcgagc tcgtagatga tctggtcatc tcccaggtaa gcgttctgca cgaaaatgtg
      601 cttttccgcc cgccggatgg cctcgagctg cgctcggtaa atctgcgcat catgagggcg
      661 cgtgcgtagc agccgcagct cctggccgcc ctccgcaccg tccgtcctgg gggtcctttt
      721 ggtggagccg agcagggcca gatcgccaag ccggtcttcc cgggccgcct gagcggcctc
      781 gctggacagc tcgcccacca caggcccggt gagctcgacc atgatgtcgt gccagtcgta
      841 gcggtactcc cggccgatgt tcatgccgcc aaggaacgcc acccgctcat cgaagatata
      901 ggtcttcgtg tgatcgccca tgaaccaggt gttgccaagc atctgcagac gcacgccgga
      961 accttccttc aggtaggcac ccatggacac gggcatgacg taatcggccg gttgcgagtc
     1021 ggccgttgcc aacgtgccac cgatggtgcc gaggccgtcc gccatcaccg tcacggcaac
     1081 ctcgcgcgat cgccgccgca gcagatcggc gatggcgacc gcatagtcgt cgttgtcgaa
     1141 gatgtaggtc cggatctcga tggatcggcg cgcggccgct atctcggcga tgagcctgtc
     1201 gaagaacgct tcaccatcca ccagaagttt catctgcccc cgcgtgcgtt cggttcccgt
     1261 aatccggtcc aggtcgttct cccactcctc gatatccatg cccctcgacg aggaaagtgg
     1321 acgagccgcc ttgcttgcca gcctgggtac caggacgaag tcgagagcaa gctccttaac
     1381 gatccccgtg gctccagtaa acgtggccgc aagcagattg cccaccgagg taagcggccg
     1441 ccgaatgaag tcacgagtgt ggctgcgcgc cacgtgggcg gtgagctgca tgccggcggc
     1501 aagccgctga tcgggcggtt gatccggcgc gagctcccga tccgagttgg cagccaccga
     1561 tgcctcccgg aacgcctcag acacgcgcac cgtatcgacc ccggcctggg cgatttggca
     1621 aaccagagct gcggacacca gtgccagcaa ataccacttc tgcttcacct cgaattctcc
     1681 tcaccgttca gatttccgag gttcaacggt agatgccgcg cggcagaccc gcctgagggg
     1741 gcg
//
LOCUS       DBNHKV010000100         7384 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_227676, whole genome shotgun sequence.
ACCESSION   DBNHKV010000100 DBNHKV010000000
VERSION     DBNHKV010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7384
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_227676"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="AC16PP_03235"
     CDS             <1..232
                     /locus_tag="AC16PP_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480667.1"
                     /translation="RNARLFLEVLARAGRTPTQANFRYSLESLGPWTDPLIDMPPIIF
                     SPESHQGLHDVFLAQAQSGRWVIVPQISPTRL"
     gene            complement(274..350)
                     /locus_tag="AC16PP_03240"
     tRNA            complement(274..350)
                     /locus_tag="AC16PP_03240"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(314..316),aa:Pro,seq:cgg)
     gene            complement(405..1577)
                     /locus_tag="AC16PP_03245"
     CDS             complement(405..1577)
                     /locus_tag="AC16PP_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6480668.1"
                     /translation="MTGFAIVLAKECRDNIRDRRTIISSLSLAVLGPALFVGLMAFVL
                     DSAVGESSDPVEITVVGADHAPGLMAFLETQNTRIERVDLEDPRGAVAGGSRKLVLSI
                     PDDYSERFAAGQVTTVSLIYDSSDMGMSRRNFRMAQQFILAHARMIGLLRLQLRGVDP
                     AIASPVQVQAVDTASPAARALAILATLPYLLVLVIFMGGFYLAIDATAGEREHGSLEP
                     LLTQPISRGALVLGKVAATAVFSAASLALFLASLAISLPYAPLHKVGMSLHIDVSTAL
                     TVFVMALPLILFAAALLNVVASFAKSYKEAQTYLTFVILIPTLPLIITRMMNVEPSAA
                     LMMIPSLSQGTLINDAVAGEPVQWVFLLISVVSTTLTAGALTMLAVYLYKRERILI"
     gene            complement(1574..2299)
                     /locus_tag="AC16PP_03250"
     CDS             complement(1574..2299)
                     /locus_tag="AC16PP_03250"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6480669.1"
                     /translation="MIRAEGLDKSFGEVTAIRHVSFDAADGKVTALLGPNGAGKSTSL
                     RILATVLQPDGGKAEVDGVDVVADPMAVRRQIGVLPHAAGLYARLTARENVAYFGSLH
                     GIAKSRLSERIDELIELLEMGEFAERRVAGFSQGQRIKVALARALVHDPRNVLLDEPT
                     TGLDVMATRALRDIIIRLRDEGRCVLFSSHIMQEVANVCDDVIVIGHGRVLFQGTLDG
                     LRDHAGEADLEEAFIRVIDEEDV"
     gene            complement(2296..3753)
                     /locus_tag="AC16PP_03255"
     CDS             complement(2296..3753)
                     /locus_tag="AC16PP_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6480670.1"
                     /translation="MRPAPSILLACLCFLPFASALAAAPALEQCELSGSHGVGRVEAV
                     CGRLPRPENPEQPDGARLDLFIAKIPSLSPEPAPDAFTIINGGPGASSVSLFVDLAQA
                     FSGVLRERDIVLVDQRGTGRSNALECPELEKAAEDYSLEAVQEATRACLESLAGDPRF
                     YTTSQAVQDLEAVRAALGYERLNVYGVSYGTRVSMHYLRRYPDRVRTLILDGVVPPGL
                     ALGVNVAENAQRRLDELFAQCDRAPECSEAFPDLQASFTQLQSNLNAEPVSLRFPNPI
                     TGLEESLAFGYDHLALVVRLLSYAPETASLLPLIIDQAAGGNYLPAASQALRITDALS
                     GTMSFGMHNSVTCTEDLPFIDFSQVNWDALEATYIGADQVRALRAICEIWPRGAMDPD
                     LKDPVQADTPTLILSGELDPITPPAYGDQIKPGFGNSMHIIGAGQGHGVVSRGCLPGL
                     VTQMVEAGHLGDLDAGCVERLSAAPFFIDLMGPAP"
     gene            complement(3756..4781)
                     /locus_tag="AC16PP_03260"
     CDS             complement(3756..4781)
                     /locus_tag="AC16PP_03260"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR6480671.1"
                     /translation="MPGDPAIATTTTRISADPNDALTPVYDLARDDFAEVNRLIPHQL
                     TSDVELVEEIGRYIVDSGGKRLRPLMVLLGARCCGYQGDDHVRLAAIIEFLHTATLLH
                     DDVVDRSELRRGRATANALWGNAPSVLVGDFLYSRAFQLMVELEDMDIMAILADATNT
                     IAAGEVQQFASIGNFAIDEAQYMEIIRCKTALLFQAATHTAGVLSSEDPDEVAALKNF
                     GLHFGLAYQLVDDWLDYEGDSSTLGKNVGDDLAEGKLTLPLIHALANAPDADANVVRD
                     AVSARSAERLAEVLQVVRSSGALSYTRSAATRQCARAVECLTGLPDNEYRDALETLAN
                     FCLDRLR"
     gene            4972..5283
                     /gene="rplU"
                     /locus_tag="AC16PP_03265"
     CDS             4972..5283
                     /gene="rplU"
                     /locus_tag="AC16PP_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR6480672.1"
                     /translation="MFAVFSSGGKQHRVTEGEVIRVERLTGEPGDEVVFDKVLMVADG
                     DDVSVGQPFVDGGKVTAEVVSSGRGKKIRVIKFKRRKDYMRRQGHRQWYTELKITGIN
                     G"
     gene            5301..5570
                     /gene="rpmA"
                     /locus_tag="AC16PP_03270"
     CDS             5301..5570
                     /gene="rpmA"
                     /locus_tag="AC16PP_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020291029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR6480673.1"
                     /translation="MAHKKAGGSTRNGRDSESKRLGVKKYGGQFVAAGNIIVRQRGTK
                     FHPGDNVGCGRDHTLFATADGYVQFLTRGPHKRKFVCVEPATAEA"
     gene            5619..6809
                     /gene="cgtA"
                     /locus_tag="AC16PP_03275"
     CDS             5619..6809
                     /gene="cgtA"
                     /locus_tag="AC16PP_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016713347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HIR6480674.1"
                     /translation="MQFIDEATIRVEAGKGGGGCLSFRREKYVARGGPDGGDGGDGGD
                     VILVADDSLNTLVDFRYQPLYRAGNGQPGSGRNKTGAHGDARLVRIPVGTSVIDDETL
                     ETLGDLTEAGQELLVARGGRRGLGNAHFKSSVNRAPRRTTTGEPGEVRRLRLQLKLLA
                     DVGLLGLPNAGKSTLIAQVSAARPKVADYPFTTLVPSLGVVGAGTDNSFVMADIPGLI
                     QGAAEGSGLGVQFLRHLARTRILLHLVEVAPLDGSDPIDNARLIEAELADYSQALRER
                     PVWLVLSKTDLVTDADLEGLERRFADAFPGRPLFAVSAVTTAGIGRLTDALLAAVAGI
                     RERLAGDEEYAEREAELQARIGEDVLRGAVLAKPGALRGNSEERDEADDDGEDQVQVT
                     YVPE"
     gene            6833..>7384
                     /gene="proB"
                     /locus_tag="AC16PP_03280"
     CDS             6833..>7384
                     /gene="proB"
                     /locus_tag="AC16PP_03280"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR6480675.1"
                     /translation="MRARLAAARRVVVKIGSALLTDDGAGLATSAMGSWCEQVNRLMD
                     AGVQVLIVSSGAVAEGVSRLGWSSRPVGVHEQQAAAAVGQMGLMQAYERAFAGHARHT
                     AMVLLTHDDLADRERYLNARATLTRLLDLGVIPIVNENDTVATDEIRFGDNDTLAALV
                     TNLLEADLLVILTDTDGLHERDPR"
BASE COUNT         1324 a         2286 c         2533 g         1241 t
ORIGIN      
        1 tcgcaatgcg cggctctttc ttgaggtgct ggcgcgtgcc ggacgcactc ccacccaggc
       61 taattttcgc tattcgctgg agtccttagg gccctggacg gatcccttaa tcgacatgcc
      121 gccgatcatc ttctctcctg agagtcacca aggcctgcac gacgtgtttc tcgcccaagc
      181 gcaaagcggc cgctgggtga tcgtgccgca aatcagcccg acgcggcttt agtgctctgg
      241 ggaaatcggc tggggattat ttacgcgggc aattggtcgg agagagagga tttgaacctc
      301 cggcccccac gtcccgaacg tggtgctcta ccaagctgag ctacactccg actgcccctg
      361 gtgagggccg cgtattgtaa aagcgccccg gcgcgggaac aacatcagat caatatgcgt
      421 tcgcgcttgt aaaggtacac ggccagcatc gtcagcgccc cggccgtcag cgtcgtcgag
      481 accacggata tcagcagaaa aacccattgc acgggttcac ccgccaccgc gtcgttgatc
      541 aacgtcccct ggctcaggct cggaatcatc atcagggccg cggaaggctc cacgttcatc
      601 attcgcgtga tgatcagggg aagcgtgggg atgaggatga cgaacgtcag gtaagtctgc
      661 gcttccttgt agctcttcgc gaacgaagcg accacgttca gcagggcggc cgcgaacagt
      721 atcagcggca gcgccatgac gaaaacggtc aacgcggtcg agacatcgat gtgcagcgac
      781 atgcccacct tgtggagggg cgcgtagggc agagagatgg ccaggcttgc gaggaacaac
      841 gccagggagg ctgcgctgaa caccgccgtg gcggctacct tgcccagcac gagggcgccg
      901 cgcgatatgg gctgggtcag caacggctcc agggagccgt gctcgcgctc gccggccgtt
      961 gcgtcgatgg ccaggtaaaa gccgcccatg aagatgacga gcaccaggag gtagggcaag
     1021 gtggctagga tggccagcgc gcgggcggcc ggcgaggccg tgtcgactgc ctgaacctga
     1081 acgggggacg cgatggccgg gtcgaccccg cgaagctgga gccggagaag cccgatcatc
     1141 ctggcgtgcg ccaggatgaa ctgctgcgcc atccgaaagt tgcggcggga catgcccatg
     1201 tccgagctgt cgtagatgag ggacacggtg gtcacctggc ccgccgcgaa gcgctcggaa
     1261 taatcatcgg gaatcgacag aaccagcttt cgggatccgc cggccaccgc accccggggg
     1321 tcttcgagat ctacccgctc gatgcgcgtg ttctgggttt ccaggaaggc catcagcccg
     1381 ggcgcgtggt cggcgccgac gacggtgatc tccaccggat cgctggactc tcccaccgcg
     1441 gagtccagaa cgaaggccat cagcccgacg aacagcgcgg gtcccaggac ggcgagagac
     1501 agcgacgaga tgatcgttcg acggtcgcga atgttgtcgc ggcattcctt ggcaaggacg
     1561 atggcgaaac cggtcacacg tcctcctcgt cgatcacgcg gatgaaggcc tcctccagat
     1621 cggcttcgcc ggcgtggtcg cggagcccgt ccagggtccc ctggaaaagc acgcgtccat
     1681 ggccgatgac gatgacgtcg tcacagacgt tggcgacctc ctgcatgatg tggctcgaga
     1741 acaggacgca gcgcccctcg tcgcgaagcc ggatgatgat gtctcggagc gcccgcgttg
     1801 ccatgacgtc gagcccggtg gtgggctcgt ccagcaggac gttgcgcggg tcgtggacga
     1861 gcgcgcgggc aagagcgacc tttatccgct gaccctggga aaaaccggcg acccggcgtt
     1921 cggcgaactc gcccatttct aaaagctcga tcagctcatc gatgcgttcg ctcagccgtg
     1981 acttcgcgat gccgtgcaga gacccgaagt acgcgacgtt ctcccgggcc gtgaggcggg
     2041 cgtacagccc tgcggcgtgc ggcagcacgc cgatctgacg ccgcaccgcc atggggtcgg
     2101 cgacgacgtc gacgccgtcg acctcggctt tccccccatc gggttgcagc acggtggcca
     2161 gaatcctgag cgaggtggac ttcccggcgc cgttggggcc gagcagggca gtgactttgc
     2221 cgtcggccgc atcgaacgag acgtgacgaa tggcggtgac ctcgccaaac gatttgtcga
     2281 gcccctcggc gcggatcatg gggctggacc catcaggtcg atgaagaatg gtgccgcgga
     2341 caggcgctcg acgcagccgg cgtccaggtc ccccaggtgc cctgcctcca ccatctgcgt
     2401 gacgagaccc ggcagacatc ccctggatac gacgccgtga ccctgacccg caccaatgat
     2461 gtgcatcgag ttgccgaagc ccggctttat ctggtccccg tatgcgggcg gtgtgatggg
     2521 gtccagctcg ccggacagga tgagcgtggg cgtgtcggcc tgaacgggat ccttcaggtc
     2581 cggatccatt gccccgcgcg gccagatctc gcagatcgcg cgaagggccc ggacctgatc
     2641 ggcgccgata taggtcgcct ccagagcatc ccagttcacc tggctgaaat cgatgaaggg
     2701 cagatcctcg gtgcaggtga ccgagttgtg catgccgaag ctcatggtgc cgctcagcgc
     2761 gtccgtgatt cgcagtgcct gggacgccgc cggcagatag ttgccgccag ccgcctggtc
     2821 gatgatcagg ggcaaaagcg atgcggtttc cggggcgtag ctcaacaggc gcaccaccag
     2881 ggcgagatgg tcgtagccga acgccaggct ttcttccagg ccggttatcg gattggggaa
     2941 ccgcaagctg accggctcgg cgttcagatt gctctgcagc tgggtgaaac tggcctgaag
     3001 gtcaggaaac gcctcgctgc actccggtgc ccggtcgcac tgggcgaaca gctcgtccag
     3061 gcgccgctgc gcgttctcgg ccacgttgac gccgagagcc agcccgggag gcaccacgcc
     3121 gtccaggatc aacgttctga cccggtccgg ataacgacgc agatagtgca tggaaacccg
     3181 ggttccgtag gacacgccat agacgttgag cctttcgtat cccagcgctg cccgcaccgc
     3241 ctcgagatcc tgcacggcct gggaggtggt atagaaacgg gggtcgccgg cgagggactc
     3301 aaggcatgcc cgtgttgctt cctggacggc ctcgagggag tagtcctcgg cggccttctc
     3361 cagctccggg cattcgaggg cgttggaccg cccggtgcct ctttggtcga ccagaacgat
     3421 gtcccgctcg cgcagaacgc ccgagaacgc ctgggcgagg tcgacgaaca gcgacaccga
     3481 ggaagccccg ggaccgccgt tgatgatggt gaacgcatcc ggtgcgggtt ccgggctcag
     3541 ggagggtatc ttcgcgatga aaaggtcgag ccgagctccg tcgggctgtt cggggttctc
     3601 gggtcgaggc agccggccgc acacggcctc cacgcggccg accccatggc tgccgctgag
     3661 ctcgcattgc tcgagcgcgg gcgcggccgc cagcgcgctg gcgaagggca agaaacacag
     3721 gcaggccagc agaatcgagg gtgcgggacg caccgctagc ggagtcgatc caggcagaag
     3781 ttcgccagcg tctccagcgc gtcgcggtac tcgttgtcgg gaagtcccgt taggcactcc
     3841 acggcccgcg cgcattgccg ggttgccgcg ctgcgggtgt agctcaacgc gccggaggaa
     3901 cgcacgacct gaagcacttc ggcgagacgc tcggcggatc gggcgctcac ggcgtcgcga
     3961 acgacattgg cgtcggcgtc cggtgcgttt gccagcgcat gaatcagcgg cagggtcagc
     4021 ttgccctcgg ccaggtcgtc gccgacgttc ttgcccaggg tggaggagtc gccctcgtaa
     4081 tcgagccagt cgtcgaccag ctggtaggcg agcccgaaat ggaggccgaa atttttgaga
     4141 gcggcgactt cgtcgggatc ttcgctgctc agcacgccgg ccgtatgggt ggcggcctga
     4201 aacagcagcg ccgtcttgca gcggatgatc tccatgtact gggcttcgtc gattgcgaag
     4261 ttgccgatgc tcgcgaactg ctgcacctcg cccgctgcga tggtgttggt cgcgtccgct
     4321 aggatcgcca tgatgtccat gtcttccagc tcgaccatca gctgaaaagc ccgggagtag
     4381 agaaaatcgc cgacgagcac gctcggggca ttgccccaga gcgcgttggc cgttgcccgg
     4441 ccccggcgca gctcggagcg gtccaccacg tcgtcgtgga gaagggtcgc ggtgtggagg
     4501 aactcgatga tggcggcaag gcgcacgtgg tcatcgccct ggtagccgca gcagcgggcg
     4561 cccagcagca ccatcagcgg acggaggcgt ttgcctccgc tgtccactat gtagcggccg
     4621 atctcctcga cgagctccac gtcggaagtc agctggtggg gaatgaggcg attgacctcc
     4681 gcgaaatcgt ccctcgcgag gtcgtacacg ggcgtcagcg cgtcgttcgg gtcggcgctg
     4741 attcgcgtcg ttgtggttgc tatggccggg tcacctggca tcggctaact cgttgtaatt
     4801 caatatcctt gcccgtagaa ttacgcgcct ttcggcgccg tcccggggca ttttacgcga
     4861 cgggccgcca gcttaagccc gggacaatcg cttcagcaag gctcgaatta tagccctcgg
     4921 gtgggtgttc gtcgcggcat cgtgctgccg cctggataga ggagtttagg tatgtttgcg
     4981 gttttctcca gcggtggcaa gcagcaccgg gtcaccgaag gagaggtcat acgagtggag
     5041 cggctgacag gtgaaccggg cgacgaggtg gtgttcgaca aagtcctcat ggtcgccgac
     5101 ggtgacgatg tgtccgtggg ccaaccgttc gtcgacggag gtaaagtcac cgccgaggtt
     5161 gtgtccagcg gccgcggcaa gaagataagg gtgatcaagt tcaagcggcg caaggactac
     5221 atgcgtcgtc agggccaccg gcagtggtac accgagctca agatcacggg cataaacggc
     5281 taaacggcgg gaggcaggaa atggctcaca agaaagctgg cggcagcacc cggaacggtc
     5341 gcgattccga gtcgaaacgg cttggcgtca agaagtatgg cggccagttc gttgcggcgg
     5401 gaaacatcat cgttcggcag cggggcacca agtttcaccc gggcgacaac gtgggctgcg
     5461 ggcgcgatca cacgctcttt gcaaccgcgg acggctacgt tcaatttctc acccggggtc
     5521 cgcacaagcg caaattcgtc tgcgtggagc cggcgacggc ggaagcctga accagccaga
     5581 ccccgcggac gcggggtttt ttttgcgttg agcacgccat gcagttcatc gacgaagcca
     5641 cgatcagggt ggaagcagga aagggcggcg ggggctgcct gagcttccgc agggagaagt
     5701 acgtggcccg cggagggccc gacggcggtg acggcggaga tggcggcgac gtcattctgg
     5761 ttgccgacga ctcgctcaac accctggtcg atttccgcta ccagccgctg taccgagctg
     5821 gtaacggaca gccgggcagc gggcgcaaca agaccggggc tcatggtgac gccaggctgg
     5881 tgcggattcc ggtaggaacc tcggtgatcg acgacgagac cctggagacc ctcggcgatc
     5941 tcaccgaggc cggtcaggaa ctgctcgtcg ccaggggtgg ccggcggggt ctgggcaacg
     6001 ctcacttcaa atccagtgtc aatcgggccc cgcggagaac gaccaccggc gagcctggtg
     6061 aggtccgccg gctgcgcctg cagctcaagc tgctcgccga cgtcgggctg ctggggctgc
     6121 ccaacgcggg caagtccacg ctgatcgcgc aggtctccgc ggcgcggccc aaggtggcgg
     6181 attatccctt caccacgctc gttcccagcc tgggggtcgt gggcgccggt accgacaaca
     6241 gcttcgtcat ggcggacatt cccggattga ttcagggcgc tgcggaagga tccggcctcg
     6301 gggtccagtt tctgcgccat cttgcgcgta ccaggattct gctgcacctg gtggaggtcg
     6361 cgccgctgga cggctccgac cccatcgaca acgcgaggct catcgaagcg gagctggccg
     6421 actacagtca ggcactgcgc gaacggcccg tctggctcgt gctcagcaag acggacctgg
     6481 ttacggatgc cgatctcgaa ggccttgagc ggcgtttcgc cgacgctttt cccgggcgtc
     6541 ccctctttgc cgtgtcggcc gtcaccaccg ctggaatcgg caggctgacg gacgcgctgc
     6601 tggctgccgt ggccggaatt cgcgagcgtc ttgccggtga cgaagagtac gccgaacggg
     6661 aggcggagct gcaggcccgg atcggggagg acgtgctgcg cggcgccgtg ctcgcaaagc
     6721 ccggtgcgct gcgggggaac tccgaggaga gggacgaagc ggacgatgac ggcgaggatc
     6781 aggttcaggt aacgtacgtt cccgaatgag cagggatatt ccgagtgacg aggtgcgtgc
     6841 gcggctggcc gccgcccgac gggtcgtggt caagatcggc agcgcgctgc tgaccgacga
     6901 cggcgctggg ctggccacat cggccatggg gtcgtggtgc gagcaggtga accggctgat
     6961 ggacgcagga gtgcaggtgc tcatcgtgtc ctccggagcg gtggctgagg gcgtcagccg
     7021 tctgggctgg agctcgcggc ctgtgggcgt gcacgagcag caggctgcag cggccgtggg
     7081 gcagatgggt ctcatgcagg cttacgagcg cgcttttgcc gggcacgcaa gacacaccgc
     7141 catggtcctg ctgacccacg acgatctggc ggaccgcgag cgctacctca atgcccgggc
     7201 caccctgacc cgcctgctcg acctgggcgt catccccatc gtcaacgaga acgataccgt
     7261 cgccacggac gagatccgct tcggggacaa cgatactctg gcggcgctgg tcaccaatct
     7321 gctggaagcc gatctgctgg tgatcctcac ggacaccgat ggcctgcacg agcgcgatcc
     7381 gcgc
//
LOCUS       DBNHKV010000101        10700 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1289970, whole genome shotgun sequence.
ACCESSION   DBNHKV010000101 DBNHKV010000000
VERSION     DBNHKV010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10700)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10700)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10700
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1289970"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="AC16PP_03285"
     CDS             <1..321
                     /locus_tag="AC16PP_03285"
                     /inference="COORDINATES: protein motif:HMM:NF047238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR6480676.1"
                     /translation="GFCKQGEGGDYVSSGITRLGGARPNNTSGGHLCEGYTHGINMVI
                     ENVRQLRHDVDDFCPIGSDGRRVHTYDYTEGGCRQVKDCELTANLGWATPGTGSAMVM
                     ARGG"
     gene            324..845
                     /locus_tag="AC16PP_03290"
     CDS             324..845
                     /locus_tag="AC16PP_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6480677.1"
                     /translation="MALEGEYLGMPVLVDDLDQENLAFFGHCGRGELCLQQCKACGLK
                     RYPPTTACPWCASPDATWEPVAGKGTVYSYGEIHHAIQPAFRQNSPYLLLLVELDEQR
                     DLLAPHDGLRMTGNLATEDGDLAPAELVRSVGIGSRVRVVFKQLGDGIALPLWCLDES
                     AEQPQTPWRYPQE"
     gene            903..1316
                     /locus_tag="AC16PP_03295"
     CDS             903..1316
                     /locus_tag="AC16PP_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HIR6480678.1"
                     /translation="MLDPTTITIFGLVLIKLLLVASIGMHRLYIASTSDKAPNTFLPT
                     GEDVGRFAVRLARAHANCYEFLPFALAVLVYAAATESSALTDGLAYYFLIARIGQSAT
                     HLVSTSTTAVFIRFAFFLAQVVILLVWCLGFLGAL"
     gene            1405..2409
                     /locus_tag="AC16PP_03300"
     CDS             1405..2409
                     /locus_tag="AC16PP_03300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6480679.1"
                     /translation="MPELRVGVCYDFRNPPDSGVSDARLYAEVMEQVRWLDGIGADLV
                     WFTEHHFVDDGYLPSWTPVAGAMAAVTENVRFGTDICLMPFNHPVRLAEDLAVLDNLS
                     GGRVELGIGMGYAPHEFRGFGLPVSRRVSLMNEGIEVLQRCFTGERFSYHGKRYQLDD
                     VRITPGYVQPGGPPLWIAAMSEGGAARAARYEANFLPQGNRARVYDTWVDALESSGRS
                     PADHRIGIIRSILVTDDRETDWPVIRAAERYRMALYQRFFEESGEGFGDPREAIPQTW
                     IVGNVDQCVAELQQFISDFRITDIVTMAVPPGLRAEQMAPSLERLFTEVVPRLKGGRP
                     "
     gene            2406..3626
                     /locus_tag="AC16PP_03305"
     CDS             2406..3626
                     /locus_tag="AC16PP_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6480680.1"
                     /translation="MRVVVIGAGIGGSALALALDRLGIEHVVLEQAPEFTDVGAGIQL
                     SPNGVRILESLGLRDALKGFCTTPDFHKYSVWDTGETILRTALMPRVREAYGFAYYHA
                     WRPDLIAALTNNLSAGVNMNRKVVRVGQDDGKAWAECEDGERVEGDVLIGADGIHSLV
                     REQVFRPDSPRASGYVTWRGVVESETVADLDIPVSAYVDMGPRLSFVYYYVASGRLLN
                     WLALGQANDDKRESWSQTASKSEVLAAFDGWYERPRRLIEATEQPFVTALHDRNPLES
                     WVSGRIAVMGDAAHAMLPYHAQGAVQSIEDAWVLARTLELHGDDPHGALTRFESLRYQ
                     RANLLVQQSRNAERWYHLDDPVEVAARNERFRRNNDRYGDDFSPQQHWLYSYDAEAAA
                     RGTDEEWRALPTWS"
     gene            complement(3636..4193)
                     /locus_tag="AC16PP_03310"
     CDS             complement(3636..4193)
                     /locus_tag="AC16PP_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013053.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIR6480681.1"
                     /translation="MQRLDPKSTAVIVIDVQERLAPAMPEAHLAELLRASRILLRGAA
                     LFGSPVLATEQYPRGLGATVPELGEQLGELNVAPREKLSFSACDDEAFMAELEASGAR
                     AAVVVGMESHVCVYQTVRDLVGAGLDVHVPIDGVCSRRDDHRQAGIDLCVRAGAVRTT
                     TESVAFDWLRVAGSPEFKELSGLIR"
     gene            complement(4214..7597)
                     /locus_tag="AC16PP_03315"
     CDS             complement(4214..7597)
                     /locus_tag="AC16PP_03315"
                     /EC_number="6.3.4.14"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase family protein"
                     /protein_id="HIR6480682.1"
                     /translation="MEKLLIANRGEIAVRIARAAAELGLVTVAVYAEDDAASLHRSRA
                     DESEALTGSGPAAYLDIGQMVEVACRSGCDAVHPGYGFLSENPDFAAAVEAAGLTFVG
                     PTPETLALLGDKVSARRLAGTCGVPLLAGSQAPVTLEEARAFLAGLPDGVDMVIKAVA
                     GGGGRGMRVVGSAADLEEAFARCCSEARLAFGDDRVYVEEFLPKARHLEVQVLGDGTG
                     AVSHVWERECSIQRRHQKIVEVAPSPTIGPQLRESLLRAAVDIGEAARYRGLGTIEFV
                     VDGSGERYAFIEANARLQVEHTVTEEVTGLDLVRAQLEVAAGRTLAELSLEQRDIPVP
                     RGYAAQARINMETMLADGTTRPGGGTLTSFEVPTGPGLRTDTFGYVGYTTSPAYDSLL
                     AKLIVHSPAGSFADCARKGYRALCETRIEGVATNLDFLRNLFAQPAFQASEVHTGYVD
                     EHVDELVKEAGHRVYHGGRAAAAPATPVLAGVKVDAADPLAVLDHGKSGGASVAAAPR
                     AIALVDEELPAGVSVIRAPMQGTVVSFEVAPGDEVYVGRQVLVMDAMKMEHVVKAEVS
                     GIVQRLGVNEGDTVFEGHPLVYLQEADIAAGEEAGQEAFDLDYVRPDLAEVFERKAAG
                     HDENRPEAVAKRHGRGHRTARENVADLCDPGSFVEYGSVVVAGQRRRRSMEDLIKNTT
                     GDGMVCGLGRVNGDLFGPERSRAMVMSYDYMVLAGTQGIKNHAKKDRLFEIAEAQRLP
                     TVLFAEGGGGRPGDTDGSGVAGLDCWAFTYFARLSGLVPIVGITNGRCFAGNAVLLGC
                     CDVIIATQGSNIGIGGPAMIEGGGLGIFKPEEVGPMDVQVPNGVVDIAVEDEAQAVAV
                     AKKYLSYFQGPVEDWDAPDERKLRFVVPENRLRYYDMREVIAGIADVGSVLELRQGWG
                     PGIITAFIRVEGMPLGVIANNPGHLSGAIDAEGADKGTRFMQLCDAFDIPILSLCDCP
                     GIMVGPESEKTALVRHAARMFVVGANIDVPLMTIVTRKGYGLGAQAMAGGSFKAPLFT
                     VTWPTGEFGGMGLEGAVKLGYRKELQAIDDPAERKDAYEKMVADMYQRGKAVNMASHF
                     ELDEVIDPAESRRWIVAGLRSVPPAPRRSGKKRPNVDTW"
     gene            7774..8478
                     /locus_tag="AC16PP_03320"
     CDS             7774..8478
                     /locus_tag="AC16PP_03320"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6480683.1"
                     /translation="MTVTLSASGLTKVYQTGDVVVHALRGAELELREGELVVMLGPSG
                     SGKSTLLNILGGLDVPTAGTVWFRNRQLTASDEASLTEYRRRHVGFVFQFYNLIPSLT
                     ARENVALVTEISSNPMDAAEALALVGLAERLDHFPAQLSGGEQQRVAIARAIAKRPEI
                     LLCDEPTGALDSETGILVLAAIDQVNRELGTTTAVITHNTVIGAMADRVMHMADGLIA
                     RVDENPDRQPPDELSW"
     gene            8502..>10700
                     /locus_tag="AC16PP_03325"
     CDS             8502..>10700
                     /locus_tag="AC16PP_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6480684.1"
                     /translation="MLRDLVHMKGQVIAVGLVIASGVAVLVMALSTLEALRETTDAYY
                     ERYRLADVFAGVKRAPEPLSDRIREIEGVQTVQTRIVKFATLDVLHFPEPVMGQFVSL
                     PESGPPLLNQLALRAGRHVEPGQHDEVVVNEPFAEAHGLTPGDSLVAVLNGKRRLLRV
                     TGIALSPEFIYSLGPGALMPDDERFGVIWMNRKALAAAFDLKDAFNNISLQLTRGTRP
                     EGVITQLDRLLEQYGGIGAVARANQMSNWFVMNEIDQLATISRILPSIFLLVSAFLTN
                     MVLTRRIDTERAQIGLMKAFGYTSFEVGFHYTKLVLGMAVVGIVVGLFVGGWFGRLNT
                     QMYAEVFRFPLLLYRPSPSAFAIASALSLLAALAGAMSSVRRATRLPPAQAMQPPAPP
                     TFRRGRLAHSRFGRWLDQPTRIVLRNIARWPGRAALTTTGIAASVALLVLALQWNDSL
                     NYLAQSYFFQAQRQHVMIGLADTRSATVMHDIEQLPGVLAAEPMRIVSADLSRGAVTH
                     RGALTGVADGAQLQPIYDDARGVEIPTPPEGLVLGITLAEKLGVGVGDRIWVNVLEGR
                     RPNVQLPVVEVVETYIAMPAYIHIDALNRLLKERPSVEYANLRIDRLEESRLYAELKK
                     LPVVSAVMLRQAAIDAFYDNVVEHLMVFITMFSSLACILGFGVAYNSVRIALSERGRE
                     LATLRVLGFTRGETSYILLGEVALLIVAALPLGCLLGRLLSQLIAAAFNTE"
BASE COUNT         1747 a         3701 c         3474 g         1778 t
ORIGIN      
        1 ggcttctgca agcagggcga gggcggcgac tacgtgagct ccgggatcac gcgcctgggt
       61 ggggcccggc cgaacaacac gagcggcggc cacctgtgcg agggctatac ccacggcatc
      121 aacatggtga tcgagaacgt ccggcagctg cgccacgacg tcgacgactt ctgcccgatc
      181 ggttccgacg ggcgtcgcgt gcacacctac gactacaccg agggcggctg ccgccaggtg
      241 aaggactgcg agctgacggc caacctcggc tgggcgacgc ccggtaccgg ctcggccatg
      301 gtcatggcga gaggaggctg agaatggcgc tcgaaggcga gtatctcggc atgcccgtgc
      361 tggtcgacga cctggaccag gaaaacctgg cgttcttcgg ccattgcggc cggggcgagc
      421 tgtgcctgca gcagtgcaag gcctgcgggc tgaagcgtta cccacccacc acggcgtgcc
      481 cgtggtgcgc gagcccggac gccacctggg aaccggtggc cggcaagggc accgtgtact
      541 cctacggcga gatccaccac gccatccagc ccgcattccg ccagaactcc ccgtacctgc
      601 tgctcctggt ggagctcgac gagcagcgcg acctgctggc cccacacgac gggctgcgca
      661 tgacgggcaa cctggcaacc gaggacggcg acctggcgcc cgccgagctc gttcgcagcg
      721 taggtatcgg cagccgcgtg cgggtagtgt tcaaacagct gggcgacggc atcgcgctgc
      781 cgctttggtg cctggacgag tccgccgagc agccacagac cccctggcgt tacccccagg
      841 agtgatcgcc gacatctgcc cgacgcctcc cacacgaaac aacaaggcaa ggagagaatt
      901 caatgctcga ccccaccacc atcaccatct tcggtctggt tctgatcaag ctgctgctcg
      961 tcgccagcat cggcatgcac cgcctgtaca tcgcatcgac gtctgacaag gcgcccaaca
     1021 cctttctgcc caccggcgag gacgtcggcc ggtttgccgt ccggctggcc cgggcccacg
     1081 ccaactgcta cgagtttctg cccttcgctc tggccgtcct ggtctacgcg gcggccacgg
     1141 aaagcagcgc gctcaccgac gggctcgcct actactttct gatcgcacgc atcggacaat
     1201 cggccacgca cctggtttcg acgagcacga cggccgtgtt catccgcttc gcgtttttcc
     1261 tggcgcaggt ggtgattctg ctcgtctggt gcctgggctt tctcggcgcg ctctgagctc
     1321 ggcgaaggcg tcgccggccg ggcgcagcgt tgagggcatg gcacgcatgc tatgctcggg
     1381 cccatgcccc agccggatat tcccatgcct gaactgcgag tcggcgtctg ctacgacttc
     1441 cgcaatccgc ctgattccgg agtgagcgac gcgcggctgt acgccgaggt catggagcag
     1501 gttcgctggc tggacggtat cggcgccgac ctggtctggt tcaccgagca ccacttcgtc
     1561 gacgacggct acctgcccag ctggacgccg gtggccggcg ccatggctgc agtcacggag
     1621 aacgtgcgct tcggcaccga catctgcctg atgcccttca accatcccgt ccgcctggcc
     1681 gaggatctgg ccgtgctcga caacctgtcg ggggggcggg tcgagctcgg cataggcatg
     1741 ggctacgctc cccacgagtt ccgcggcttc ggcctgccgg tatcacgccg ggtctccctg
     1801 atgaacgagg gcatagaggt gctgcaacgc tgcttcaccg gcgagcgttt cagctatcac
     1861 ggcaagcgct accagctcga cgacgtccgt atcacgccgg ggtacgtgca gcccggcggc
     1921 ccgccgttgt ggatcgccgc catgtccgaa ggaggtgcgg cgcgggcggc ccggtacgaa
     1981 gccaatttcc tgccccaggg gaacagggcg cgcgtctacg acacgtgggt cgatgctctg
     2041 gaaagctccg gccgcagccc ggccgatcat cggatcggca tcatccgcag cattctcgtg
     2101 accgacgaca gggaaacgga ctggcccgtg atccgtgccg ccgaacgcta tcgcatggcc
     2161 ctgtaccagc ggttcttcga ggaaagcggc gagggtttcg gcgacccgcg ggaagccatc
     2221 ccccagacct ggatcgtcgg caacgtggat cagtgcgtcg ccgagctgca gcagttcatc
     2281 agcgatttcc ggatcaccga catcgttacc atggcagtcc cgcccgggct acgcgccgag
     2341 cagatggcgc cgagcctgga gcgtctgttc accgaggtgg ttcctcgttt gaagggaggt
     2401 cgcccatgag ggtcgtcgtc atcggcgcgg gcatcggcgg ctccgcgctg gcgctggcac
     2461 tggatcggct cggtatcgag cacgtggttc tcgagcaggc gcccgagttc accgacgtgg
     2521 gcgcgggcat ccagctcagc cccaacggcg ttcggatcct ggaaagcctc ggcctgcgcg
     2581 atgcgctcaa gggcttctgc acgactccgg atttccacaa gtacagcgtc tgggacaccg
     2641 gcgagaccat tctgcggacg gcgctgatgc cccgggtacg ggaagcgtac ggctttgcct
     2701 actatcacgc ctggcgcccg gacctcatcg ccgcgctgac caacaacctt tcggccgggg
     2761 tgaacatgaa ccggaaggtc gtccgcgtgg gtcaggacga cggcaaggcc tgggcggagt
     2821 gcgaggacgg cgagcgcgtc gagggcgacg tgctcatcgg cgcagacggc attcactccc
     2881 tcgtccggga gcaggtcttc cggccggact cgccgagagc gtccggatac gtgacctggc
     2941 gcggggtcgt cgagtccgag acggtggccg atctggacat tcccgtgtcc gcctacgtgg
     3001 acatgggacc gcggctgtcc ttcgtctact actacgtcgc ctctggccgg ctgctgaact
     3061 ggctcgccct gggacaggcc aacgacgaca agcgcgagtc ctggtctcag accgccagca
     3121 aatccgaggt gctggccgct ttcgacggct ggtacgagcg gcccaggcgc ctgatagagg
     3181 ctaccgagca gcctttcgtt accgcgctgc acgaccgcaa cccgctggag agctgggtgt
     3241 ccgggcgcat cgccgtcatg ggcgatgccg cccacgccat gctcccctac catgcccagg
     3301 gcgcggtgca gtccatcgag gatgcctggg tgctggcccg aaccctcgag cttcacggcg
     3361 acgaccccca cggggcgctt acgcggttcg aatcgctgcg ctaccagcgc gccaacctgc
     3421 tggtgcagca gtcgcgcaac gccgagcgct ggtatcacct ggacgatccc gtcgaggtgg
     3481 ccgcacgcaa cgagcgtttc cgccggaaca acgatcgcta cggtgacgac ttctcaccgc
     3541 aacagcactg gttgtacagc tacgatgccg aggccgctgc gcgcggaacc gacgaggagt
     3601 ggcgcgcgct gccgacttgg tcctgaccgg gccgcctacc gaatcagccc ggacagctcc
     3661 ttgaactctg ggcttcccgc cacgcgcagc cagtcgaagg cgaccgactc ggtggtcgtt
     3721 ctaacggctc ccgcgcgcac gcacagatcg atccccgcct gccgatgatc gtcgcggcgc
     3781 gagcacacgc cgtctatggg cacgtgaacg tcgagaccgg cgccaacaag gtctcgaacg
     3841 gtctggtaga cgcagacgtg cgattccatg ccgaccacca ccgcggcgcg agcgccgctg
     3901 gcttcgagct ccgccatgaa agcctcgtcg tcgcaggcgc tgaagctgag cttctcgcgc
     3961 ggcgccacgt tgagctcgcc gagctgctcg ccaagctccg ggacggtggc ccccagcccg
     4021 cgaggatact gctcggtggc cagcacggga gagccgaaca gcgccgcgcc gcgaagcagg
     4081 atgcggctgg cgcgcaggag ttccgccagg tgcgcctccg gcatggcggg cgcgaggcgc
     4141 tcctgaacgt cgatgacgat gacggcagtg gatttcgggt ccagacgctg catatgctct
     4201 ccccttgctt gggctaccag gtgtctacgt tcggccgttt cttcccggac cgccgcggtg
     4261 ccggaggcac cgagcgaagt cctgcgacga tccacctgcg agattccgcc gggtcgatga
     4321 cctcgtcgag ctcgaagtgc gaggccatgt tcaccgcctt gccgcgctgg tacatgtcgg
     4381 ccaccatttt ctcgtaggcg tctttgcgct cggccgggtc gtcgatggcc tgcagctctt
     4441 tgcggtatcc gagcttgacg gccccttcga gacccatgcc gccgaactcg cccgtgggcc
     4501 aggtcaccgt gaacaggggc gccttgaagc tgccgccggc catggcctga gcaccgagcc
     4561 cgtacccctt gcgggtgacg atggtcatca gcggaacgtc gatgttcgcg cccaccacga
     4621 acatgcgggc cgcatggcgg acgagggccg tcttctcgct ctccggcccc accatgatgc
     4681 ccgggcaatc gcacaggctc aggatgggga tgtcgaaggc gtcgcacagc tgcatgaagc
     4741 gggtgccctt gtccgcgccc tcggcgtcga tggcgccgga cagatgtccg ggattgttgg
     4801 caatcacgcc gagcggcatc ccctccacgc gaatgaacgc ggtgatgatg ccgggccccc
     4861 aaccctggcg cagctccagc accgagccga cgtcggcgat gccggctatc acctcgcgca
     4921 tgtcgtagta gcgcagccgg ttctcgggga cgacgaatcg aagctttctc tcgtccggcg
     4981 cgtcccagtc ttcaaccggt ccctgaaagt acgacaggta cttcttggca accgccacgg
     5041 cttgtgcctc gtcctccacc gcgatgtcga ccaccccgtt gggcacctgc acgtccatgg
     5101 gcccgacctc ttcgggcttg aaaatgccga gcccgccccc ctcgatcatc gccggcccgc
     5161 cgatgccgat gttcgaaccc tgcgtggcga tgatcacgtc gcaacagcct agaagcacgg
     5221 cgttgccggc gaagcagcgc ccgttggtga tgccgacgat gggcaccagg cccgagaggc
     5281 gggcgaaata agtgaaggcc cagcaatcca ggcccgcaac gccggacccg tcggtgtcgc
     5341 ccgggcggcc accaccgcct tcggcgaaca gcacggtggg caggcgctgg gcttcggcga
     5401 tctcgaacag acggtctttc ttcgcgtgat tcttgatgcc ctgggtcccg gcaagcacca
     5461 tgtagtcgta ggacatcacc atggcgcggg accgttcagg gccgaacagg tcgccgttga
     5521 cccggccgag cccgcagacc atcccgtcgc ccgtggtgtt tttgatcagg tcctccatgc
     5581 tccggcggcg acgctgtccg gcgaccacca cggatccgta ctccacgaac gaacccggat
     5641 cgcacagatc ggcgacgttc tcccgcgccg ttcggtgtcc cctgccgtgg cgtttggcca
     5701 cagcctccgg gcggttctcg tcgtgcccgg ccgccttccg ttcgaacacc tccgcgaggt
     5761 ccgggcgaac gtagtccaga tcgaacgctt cctggcccgc ctcctcgccc gcggcgatgt
     5821 ctgcttcctg cagatagacg agggggtggc cctcgaagac cgtgtcgcct tcattcacgc
     5881 ccaggcgctg gacgatgccg ctgacttccg ccttgaccac gtgctccatc ttcatggcgt
     5941 ccatgaccaa cacctgcctg ccgacgtaga cctcatcgcc gggtgccacc tcgaagctga
     6001 cgacggttcc ctgcatgggc gcgcggatga ccgaaacccc cgcgggcagc tcttcgtcca
     6061 ccagcgcgat ggcccgcggg gccgctgcga cgctcgcccc gccagactta ccgtgatcga
     6121 gaacggcgag cggatccgcg gcgtccacct ttacgccggc cagcacgggc gtggccggcg
     6181 cagcggcagc gcgtcctcca tgatagaccc tgtggcccgc ttctttgaca agctcgtcca
     6241 cgtgctcgtc cacgtagccc gtgtgcacct cgctggcctg gaaggcgggc tgggcgaaga
     6301 gattgcggag gaagtccaga ttggtcgcca ccccctcgat gcgggtctcg cagagggccc
     6361 gatagccttt gcgggcacag tcggcaaagc tgcctgcggg ggagtgaacg atgagcttgg
     6421 ccaggagcga gtcataggcc gggctcgtgg tgtagccgac ataaccgaag gtgtccgtac
     6481 gcagaccggg gccggtaggc acctcgaagc tcgtcagcgt gccgccaccg ggcctggtgg
     6541 tgccgtccgc tagcatggtc tccatgttga tccgcgcctg ggccgcgtag cccctgggca
     6601 ctgggatgtc gcgctgctcg agggagagct cggccagggt gcggccggcg gcaacttcca
     6661 gctgggcgcg aacgaggtcc aacccggtca cttcctcggt gaccgtgtgc tcgacctgca
     6721 gccgcgcgtt ggcttcaatg aaggcgtagc gctcaccgga accgtcaacg acgaactcga
     6781 tggtgccgag gccccgataa cgtgcggcct ccccgatgtc cacggcggcg cgcagcagcg
     6841 actcgcgcag ctgaggaccg atcgtcgggc tgggcgccac ctccacgatc ttctgatggc
     6901 gccgctgtat gctgcattcg cgctcccaga cgtggctcac ggcgccggtg ccgtcgccca
     6961 gcacctgcac ctccaggtgg cgggctttgg gaaggaattc ctctacgtag acgcgatcgt
     7021 caccaaaggc gagcctggct tccgagcagc agcgcgcgaa agcctcttcc agatcggcag
     7081 cgctgccgac cacccgcatg ccccggccac cgccgccggc gacggccttg atcaccatgt
     7141 ccacgccgtc cgggaggccg gcaaggaagg cgcgggcctc ctcgagcgta accggcgctt
     7201 ggctgcccgc cagcagcggc acgccgcagg tgccggcaag ccggcgcgcg ctcaccttgt
     7261 caccgagcag cgcgagggtt tccggagtcg ggccgacgaa ggtgagaccg gccgcctcca
     7321 cggctgcggc aaaatcgggg ttctcgctca aaaagccgta gccaggatgg acggcgtcgc
     7381 atcccgaccg gcacgccacc tccaccatct ggccaatgtc caggtaggcc gcggggccgc
     7441 tgcccgtgag cgcttcggat tcgtccgccc gggagcggtg cagggaggca gcatcgtcct
     7501 cggcataaac ggcaacggtg accaggccga gctcggcagc cgcacgggcg atacgaacgg
     7561 ctatctcgcc ccgattggca atcagcagtt tctccaagct ccctcctcga ccgttcccgt
     7621 gagatgggcg gccagctccc cgaaaggcag aaaaacgatc ggcgattgtc tgacgttgat
     7681 cagtcgaagt ccaacgggca acctcgtgaa agtgcggatg gtggttctgc ccgcagaatc
     7741 atctaatctg ggcttcccga cgaaggcgaa accatgactg tcaccttgag cgcaagcggt
     7801 ctcaccaagg tctaccagac cggggacgtc gtcgtgcacg cgctgcgcgg ggccgagctc
     7861 gagctgcgcg aaggtgagct ggtggtgatg ctgggccctt cggggtcggg gaagtccacg
     7921 ctgctgaaca tcctcggcgg tctggacgtg cccacggccg ggacggtctg gttccgcaat
     7981 cgccagctga cggcctccga cgaggcctct ctcacagagt accggcgccg gcacgtgggc
     8041 ttcgtcttcc agttctacaa cctgattccc agcctgaccg cccgggaaaa cgtcgccctg
     8101 gtcaccgaga tctcctcgaa ccccatggac gctgcggagg ccctcgcgct ggtcggcctc
     8161 gccgagcgtc tggatcactt cccggcccag ctctccggag gcgagcagca gcgtgtggcc
     8221 atcgctcggg cgatcgcgaa gcgcccggag atcctgctct gcgacgagcc gaccggggcc
     8281 ctggacagtg aaaccggcat cctcgtgctg gccgccatcg accaggtcaa ccgtgagctc
     8341 ggcaccacca cggccgtgat cacccacaat accgtcatcg gtgccatggc cgaccgggtc
     8401 atgcacatgg cggacgggct gatcgcccgc gtcgacgaga atccggaccg ccagcccccc
     8461 gacgagctga gctggtaact gtgcgggcgc tcgatacgaa ggtgctccgc gacctcgtcc
     8521 acatgaaagg acaggtcatc gccgtcggcc tggtcatcgc atccggggtg gcggtgctgg
     8581 tgatggccct gtccaccctc gaggcccttc gcgagaccac cgacgcctac tacgagcgtt
     8641 accgcctggc ggacgtgttc gccggggtca aacgggcgcc ggagcctttg tcagacagga
     8701 ttcgcgagat cgaaggcgtg cagacggtgc agacccgcat cgtcaagttc gcaacgctgg
     8761 acgtcctcca cttcccggaa ccggtgatgg gccagttcgt ttccttaccc gaaagcggcc
     8821 cgccgctgct gaaccagctc gcgctgcgtg ccggaaggca tgtcgagcca ggccagcatg
     8881 acgaggtggt cgtcaacgag ccgttcgccg aagctcatgg gctcacgccc ggcgactccc
     8941 tggtggcggt gctgaacgga aagcgccgct tgctccgcgt gacggggatc gctctctctc
     9001 ccgagttcat ctacagcctg gggcccggcg ctctgatgcc cgacgacgag cgcttcggcg
     9061 tgatctggat gaaccgcaag gcgctcgccg ccgccttcga cctcaaggac gcgttcaaca
     9121 acatctccct gcagctcacc cgcggaacca ggccggaagg cgtgatcacg cagctcgatc
     9181 ggctgctcga gcagtacggg ggcatcggcg ccgtcgcccg cgccaaccag atgtcgaact
     9241 ggttcgtcat gaacgagatc gaccagctgg ccacgatatc gcgcattctt ccgagcatat
     9301 ttctgctggt ctccgccttc ctgaccaaca tggtgctcac ccggcggatc gacacggaaa
     9361 gagcccagat cgggctgatg aaggccttcg gatacacgag tttcgaggtg ggctttcact
     9421 acacgaagct ggtgctgggc atggccgtgg tgggcatcgt cgtgggcctg ttcgtcggcg
     9481 gctggttcgg ccgtctcaac acgcagatgt acgccgaagt attccgcttc cccctgctcc
     9541 tctatcggcc gagcccgagc gcgttcgcca tcgcgtcggc attgagcctg ctggcagcgc
     9601 tcgcaggtgc catgagcagc gtccgccggg cgacccgtct gcccccggcc caggccatgc
     9661 agccgccggc gccacccacg tttcggcggg gacggctcgc ccattcgcgc ttcggccgct
     9721 ggctggacca gccaacccgc atcgttctgc gcaacatcgc ccgttggccc ggccgcgccg
     9781 cgttgaccac caccgggatc gccgcttcgg tcgccctgct ggtcctcgcg ctgcagtgga
     9841 acgactccct gaactacctg gcgcagagct acttctttca ggcccagcgc cagcacgtga
     9901 tgatcgggct tgcggatacc cggtccgcca cggtgatgca cgacatcgag cagctcccgg
     9961 gggtgctggc agcagagccc atgcgcatcg tcagcgcaga cctatcccga ggagcggtaa
    10021 cccaccgcgg cgcgctgacg ggcgtcgccg acggcgcgca gctgcagccg atctacgacg
    10081 acgcgcgggg cgtggagatt ccgacgccgc ccgaaggcct ggtgctgggc atcaccctgg
    10141 cggagaagct gggcgtgggc gtgggcgacc ggatctgggt aaacgtgctc gagggacggc
    10201 ggcccaacgt tcagctgccc gtcgtcgagg tggtggaaac ctacatcgcc atgccggcct
    10261 acatccacat cgacgcgctc aaccgcctgc tcaaggaacg cccgagcgtg gagtacgcga
    10321 acctgcgcat cgaccgcctc gaagagtcca ggctgtacgc cgagctcaag aagctgcccg
    10381 tggtctctgc ggtgatgctg cgccaggcgg ccatcgacgc cttctacgac aacgtggtgg
    10441 agcatctgat ggtgttcatc accatgtttt ccagcctcgc ctgcatactc gggttcggtg
    10501 tcgcctacaa cagcgtgcgt atcgctctgt cggaaagggg gcgcgagctg gctaccctgc
    10561 gggtgctcgg cttcacccga ggggagacgt cctacatcct cctgggcgag gtggcgctgc
    10621 tcatcgtggc cgccctgccg ctgggctgcc tgctcggccg actcctctcc cagctgatcg
    10681 ccgccgcctt caataccgag
//
LOCUS       DBNHKV010000102         2930 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_368874, whole genome shotgun sequence.
ACCESSION   DBNHKV010000102 DBNHKV010000000
VERSION     DBNHKV010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_368874"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1590)
                     /locus_tag="AC16PP_03330"
     CDS             complement(<1..1590)
                     /locus_tag="AC16PP_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR6480685.1"
                     /translation="MKRLVAGIVGVLLATVFLVIAAVAVLMLSEPGSRFLVERATRLL
                     PLEVEGVTGSLVGEMRFQRLRYELEGQIVEAQGLEVAFDPMAFLLKDEVRFRYVRAVR
                     LRVETRGGEATSAGLGRFELDAPPITLVIDSLAVQVAEILDLPPSSMTLAGRWGAREL
                     SLAQVAVASEVLELRLSGSLANGREPALDLVGSWSLPGTSWSGSGTLSGPLAALNVRH
                     RLEGPVTLEAQGTVDLSSLPDPRFELEVAMPEFSSEGFLLSDVLATVSGGRERVEGVV
                     EGLVSTPWLTEPVPARLSGSADLPPGEVRIAVDGFQAEVLGRPVEGRLVARQLAAGWR
                     LEDVELDLSGSRVSGSAELDESGARASVHISAPDLASLDLGVAGDASGSVDFAAEAGS
                     LSVSVSVSGSELSSGTARLLNYSVSGESRGREMNFVVRGDRAAQAGIEARMPSLQVDG
                     PIDAPAWQLEWQGGAANGIATREGEVLNLRVEALRFVALESSWRLSAPVKASADAAGV
                     RVSAFCIEGPGSSGCVDELEMS"
     gene            complement(1587..>2930)
                     /locus_tag="AC16PP_03335"
     CDS             complement(1587..>2930)
                     /locus_tag="AC16PP_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0097347 - TAM protein secretion complex
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HIR6480686.1"
                     /translation="LLGNPGLASGERFSHASYETAKGRVLDLARDLGYLDAAFNRNRV
                     LVDAEAGLADVELALESGLRYRFGPIEIEQDDLRPKLFDRFLTVSEGDDFDGRELRDT
                     QRRLMESDYYDRVLVAPVLDRREDGRVPISVQADASSRRNILLGAGFATDTGPRVRAD
                     ARYRRVNRKGHRARGGLLGSKVLSEANVEYGLPYGDPTHQWLILKGSVAYEETETARS
                     FRQTVGVARTHRRGDHWTETNYVEYRFEDFDVGDQEGDSDLLLIGTSWSYTSTVDAPR
                     ALSGRTITFDVHGASKALASDTDFLKLVVHGKQIVPLGERFRLIGRISSGWTFKSDFD
                     ELPPSERFFAGGDSSVRGYEYESLGPEENDAVVGGTRLLTGSLELDMLIRPKWSVALF
                     VDSGSAFNDSPDFSTGVGAGLRWYSPVGPIRLDLAHPLDDPDNDFRIHISFGPDL"
BASE COUNT          546 a         1049 c          895 g          440 t
ORIGIN      
        1 gctcatctcg agctcgtcga cgcagccact cgagcccggc ccttcgatgc agaaggcaga
       61 cacccgcacc ccggccgcgt ccgctgaggc cttaaccggc gcgctcagcc gccagctcga
      121 ctccagagcg acgaaccgca acgcctcaac gcgcagattc agaacctctc cctcacgggt
      181 ggcgatcccg ttggctgcgc cgccctgcca ctcgagctgc caggctggcg cgtcgatcgg
      241 accgtcgacc tgcaggctcg gcatgcgggc ctcgatcccc gcctgcgccg cgcgatcgcc
      301 acgaacgacg aagttcatct cgcggccgcg actctcccca gacaccgagt agttcagcag
      361 cctggccgta ccgctggaga gctcgcttcc cgagacactc acggacaccg agagcgaccc
      421 cgcctccgcg gcaaaatcga cggagccgga cgcgtcaccg gcgaccccga gatccagaga
      481 ggccagatcc ggtgcgctga tgtgcaccga ggcacgcgcg ccggactcgt cgagctccgc
      541 agacccggac acacggctgc cgctcaagtc aagctccacg tcctcgagcc gccatcccgc
      601 tgccagctgc cgcgccacca gccgaccctc cacgggcctg cctagcacct cggcctggaa
      661 accgtccacg gcaatgcgaa cctcgccggg cggaaggtcc gcactgccgg acaggcgagc
      721 aggcacgggc tccgtgagcc acggtgtcga gacgagcccc tcgaccacgc cttccacccg
      781 ttcgcggccg ccgctcacgg tggccaggac gtccgacagc agaaagcctt cactcgaaaa
      841 ttcgggcatc gcgacctcga gctcgaaacg gggatccggg aggctcgaaa ggtctaccgt
      901 accctgcgct tccagcgtga ccgggccttc caggcgatgt cgaacattca gggcggcaag
      961 cgggccgctc agcgtgccag agccactcca ggatgtgccg ggcagcgacc acgaaccgac
     1021 caggtcgagc gccggttcgc ggccgtttgc cagtgatccg gaaagtcgga gctccagcac
     1081 ttccgaggcc accgccacct gagcgaggct cagctctctg gcgccccagc gcccggccag
     1141 cgtcatgctc gacggcggca gatccagaat ttccgccacc tgcacggcca gcgagtcaat
     1201 caccagggta atcggcggcg cgtcgagctc gaaccggcca aggcctgccg atgtcgcttc
     1261 tcccccacgg gtttcaacgc gcaagcgcac ggcccgcacg tatcggaaac gcacttcgtc
     1321 tttgagcagg aaggccatcg gatcgaaggc gacctcgagt ccctgggcct cgacgatctg
     1381 cccttcgagc tcgtagcgca gacgctggaa gcgcatctcg ccaaccaggc ttcccgtaac
     1441 gccttctacc tcgagcggaa gcaggcgggt tgcgcgctcg accagaaacc ggctccccgg
     1501 ctcgctcagc atcagcacgg cgacggcggc aatcactagg aaaacggttg ccaggagaac
     1561 gccgacaata cctgcaacaa gacgtttcac agatctggcc cgaagctgat gtgaatgcgg
     1621 aaatcgttgt ccggatcgtc gaggggatgg gccaggtcca gccggatcgg gccaaccggc
     1681 gagtaccagc gcagacccgc gcccacgccc gtgctgaaat cgggactgtc gttgaacgcc
     1741 gagcccgagt cgacgaataa ggcaaccgac cacttgggcc ggatcagcat atccagctcc
     1801 agggagcccg tcagcagccg ggtgccccca acgacggcat cgttttcctc aggaccgagg
     1861 gattcgtact cgtagccccg aacgctgctg tcgccgccag caaagaaccg ttcgctgggc
     1921 ggcagctcgt cgaagtcgct cttgaacgtc caccccgacg agatccgccc gatcaggcga
     1981 aaccgctcgc ccagcggcac gatctgcttg ccgtgcacga cgagcttcag gaaatccgtg
     2041 tccgaggcga gcgccttgct tgcgccatgc acgtcgaagg tgatcgtcct gcccgaaaga
     2101 gcacgtggcg catccacggt gctcgtgtaa gaccagctcg tgcctatcag cagcagatcg
     2161 gaatcgccct cctgatcccc gacgtcgaag tcctcgaaac ggtactcgac gtagttcgtt
     2221 tcggtccagt gatcgccccg ccggtgggtg cgcgcgacgc ccacggtctg ccggaagctg
     2281 cgggccgtct cggtctcctc gtaggcgacg ctgcctttga ggatcagcca ctgatgagtc
     2341 ggatctccat agggcagtcc gtattccacg ttggcctcgc tgagcacctt cgagcccagc
     2401 aggccccctc gagcgcgatg gcccttgcga ttgacccggc gatagcgtgc atctgcgcgc
     2461 actcgcggcc ctgtgtccgt ggcaaagccc gcaccgagga ggatgttgcg gcgcgacgag
     2521 gcatccgcct gcacgctgat gggcacccgt ccatcctcgc gccgatccag cacgggggcc
     2581 accagcaccc gatcgtagta gtcgctttcc atgagacggc gttgggtatc ccgcagctcg
     2641 cgcccgtcga agtcgtcgcc ttcggagacc gtcagaaagc gatcgaaaag cttcggacgc
     2701 agatcgtcct gctcgatctc gatcggtccg aatcggtagc gaaggcccga ttcgagggca
     2761 agctcgacgt ccgccaggcc cgcttccgca tcgaccaaca cccgattacg gttgaaggca
     2821 gcgtcgagat agccgaggtc ccgggccagg tccagaacgc gtcccttggc ggtctcgtag
     2881 gaggcgtggc tgaaacgttc gcccgaggcc aggccgggat tgcccagcag
//
LOCUS       DBNHKV010000103         2676 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1503543, whole genome shotgun sequence.
ACCESSION   DBNHKV010000103 DBNHKV010000000
VERSION     DBNHKV010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1503543"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="AC16PP_03340"
     CDS             <1..396
                     /locus_tag="AC16PP_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6480687.1"
                     /translation="EREEMDMTSADDEARIRQLHGRYFETLGSGDLDALAEHFTFPAV
                     FKGFLDDVTMVSDGASLAPTYEKLIAAAPKAARTEVLSLDAARLRPGVHMLTMQYEQY
                     GADDQLIHEGRALYLAKEVGSELKLFAVL"
     gene            439..900
                     /locus_tag="AC16PP_03345"
     CDS             439..900
                     /locus_tag="AC16PP_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480688.1"
                     /translation="MNWDALDFAVFGAMLLGAGIVYAFARRKTRNSAYRAAVGVALGS
                     AFLLVWINGAVGILGDEDDVANMMFFGVLGIGAVGAAVCRFEARGMMLALAATACAQL
                     AVAVVALAAGLGSTSPSWPQHVLVLTALFVVMWLGSAWLFRSAAREQSFTK"
     gene            complement(890..1033)
                     /locus_tag="AC16PP_03350"
     CDS             complement(890..1033)
                     /locus_tag="AC16PP_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480689.1"
                     /translation="MTDPHEEREPARTGSLEQTDMGESLRQLWWTIGATVVGLAVIAY
                     FTL"
     gene            complement(1090..2439)
                     /locus_tag="AC16PP_03355"
     CDS             complement(1090..2439)
                     /locus_tag="AC16PP_03355"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6480690.1"
                     /translation="MSDILRHPLAMDGKDPANLRGDPISPERYYSKEWMAKEWVLMWT
                     KIWHIAGREQQLQDPGDYIVHNFMHESVIIAKQKDGSLKGFYNACGHRAQRLLWSEHG
                     SQEQMRCPYHGWVWGMDGSLLDSCDRDDFPEDPVGKIRLIEVRVDTWAGLVWYTMDDE
                     APDLMTYLAPNPELYKGHQFEKTVRVNWWRVALNTNWKFWSDNFNESYHTRTVHPQVP
                     PIIDQDHFTSRYEMFPMGHNRIVQMGRPSLRDRLPKGAPDPFEATLRAWDIDPEQYPD
                     FETKALQGWKDLKAAKRRLWRERGFLHYENLNDEDLTESPFGVLFPNVAIAPGSDSFL
                     VWRWEPHPTDPEKCYFDQWTMAYPIEGQEEFVNRTAENPLELKEAELDFCDYDDGRGV
                     QDYSDQVVYQDMHLTAGQTSGWRSRGYQEPYMAAQETRVRRFHEVLHDYLAGNPPGR"
BASE COUNT          453 a          800 c          853 g          570 t
ORIGIN      
        1 gaacgagagg agatggacat gacttcagcg gatgatgaag ccaggatcag gcagcttcac
       61 ggccgatact tcgaaaccct gggcagcggg gacctggatg cgctcgccga gcattttacg
      121 tttcccgcgg tgttcaaagg gttcctcgac gacgtgacga tggtgtcgga cggggcgtcg
      181 ttggcgccca cctacgagaa gctcatcgcg gcggctccca aggccgcgcg caccgaggtt
      241 ctgagcctgg acgccgcgcg cctcaggccg ggcgtccata tgctcacgat gcaatatgag
      301 cagtacgggg ccgacgacca gctgattcat gagggccgcg cgttgtatct cgccaaagag
      361 gtgggcagcg agctcaagct gttcgccgtg ctctgagttg cccccagcga gttcggccgt
      421 gaagcgaagg aggagcaggt gaattgggat gccttggact tcgccgtttt cggggcgatg
      481 ttgctgggtg cggggatcgt ctacgcgttc gcgcgtcgga agacgcggaa ctcggcatat
      541 cgggccgccg ttggcgttgc ccttggctct gcgttcctcc tggtctggat caacggtgcc
      601 gtcggcatcc tgggcgatga ggatgacgtc gccaacatga tgtttttcgg tgtgcttggc
      661 attggcgccg ttggcgcagc cgtctgccgc ttcgaggcgc ggggaatgat gctcgcgttg
      721 gcggcgacgg cgtgcgctca gctggccgtg gcggtggtcg ccctcgccgc cggcctgggt
      781 tccacgtcgc ccagctggcc gcaacacgtg cttgtcctca ccgcgttatt cgtcgttatg
      841 tggctgggct ctgcgtggct ctttcgaagc gccgcgcgtg agcagtcgtt cacaaagtaa
      901 agtatgcaat gacggccagc ccgacgacgg ttgcgccgat ggtccaccac agctgccgaa
      961 gagattctcc catgtcggtc tgctcgaggc ttccggttct ggctggttcc ctttcttcgt
     1021 gtgggtcagt catctggtgt ttcctgtcgc gcgtcttgcc gcctgagctc ggatcgatgc
     1081 gagaaacatt cagcgccccg gcggattccc cgccaggtag tcgtgtagca cctcgtggaa
     1141 gcgccggact cgggtttcct gggccgccat atagggttcc tgataccccc tggagcgcca
     1201 accgctggtc tgaccggcgg tcagatgcat gtcctgatac accacctgat cggagtaatc
     1261 ctgaacaccg cgcccgtcgt catagtcaca gaaatccagc tctgcctctt tcagctcgag
     1321 cgggttctcg gccgtgcggt tgacgaactc ttcctgaccc tcgatgggat aagccatggt
     1381 ccattgatcg aagtagcact tctcgggatc cgtcggatgc ggttcccagc gccataccag
     1441 gaaagaatcg gaaccgggcg cgatggcgac gttgggaaac agcacgccaa aggggctttc
     1501 cgtcagatcc tcgtcgttca ggttctcgta gtggagaaag cctctctccc tccagagcct
     1561 tcgcttcgct gccttgaggt ccttccagcc ctggagcgcc ttggtctcga aatccgggta
     1621 ctgctcggga tcgatgtccc acgcgcgcag cgtcgcttcg aacgggtccg gcgcgccttt
     1681 gggtagacga tcccgcagag agggccggcc catctggacg atgcgattgt ggcccatggg
     1741 gaacatttcg tagcgcgagg tgaaatggtc ctggtcgatg atcgggggta cctgagggtg
     1801 gaccgtgcgg gtgtgatacg actcgttgaa gttatcggac cagaacttcc agttggtatt
     1861 gagcgcgacg cgccaccagt tgacgcgcac cgttttctcg aactggtggc ccttgtagag
     1921 ctcggggttg ggggcgaggt acgtcatcag gtccggtgcc tcgtcgtcca tcgtgtacca
     1981 gaccagccct gcccaggtgt ccacgcgcac ttcgatgagc ctgatcttac ccaccgggtc
     2041 ttccgggaag tcgtcccggt cacagctatc cagcagagag ccgtccatgc cccagaccca
     2101 gccgtggtag gggcaacgca tttgttcctg ggagccgtgc tcgctccaga gcaaccgctg
     2161 ggcacggtgg ccacaggcgt tgtaaaagcc tttcagggac ccgtccttct gcttggcgat
     2221 gatcacagac tcgtgcatga agttgtgcac gatatagtcg cccgggtcct gaagctgctg
     2281 ttcgcgaccg gcgatgtgcc agatcttggt ccacataagc acccactctt tcgccatcca
     2341 ctccttgctg tagtagcgct ccggggagat ggggtctcca cgaagattag ccggatcttt
     2401 cccgtccatg gccaacggat gtctgagaat gtcgctcata ggtaggacct cgtttgcgct
     2461 tcggaaatta gactgacggg ggacggaaat gtcaaaaccg cgggacggct cgtgccgatc
     2521 gcggccggag aaagctgcag cttgctaccc gccgcgcgct cagtccagat cggacacgct
     2581 ttcgtcgacg gtgtaggaac gcatgaagcg cgtgtcgaac agcttctcct cgtcctcgcg
     2641 aatctcctgc tgagcgtccg gcgcgctcaa gaccct
//
LOCUS       DBNHKV010000104         7803 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_228312, whole genome shotgun sequence.
ACCESSION   DBNHKV010000104 DBNHKV010000000
VERSION     DBNHKV010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7803
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_228312"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..2628)
                     /locus_tag="AC16PP_03360"
     CDS             complement(70..2628)
                     /locus_tag="AC16PP_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6480691.1"
                     /translation="MEILRSALIGGVLAVAVLMPEFAVAEGEGAMIEELVVTARRRAE
                     SVQDVPGTVTAIGQSTIEGAGVERVEDFIRLTPGVTLVNTAEVGDTQVNIRGINGARD
                     AENSFAFIVDGILHTNPAAFNREYPGLQQIEVFKGPQGAIYGRNAAAGAVIVTTERPT
                     EEFTGSIKTSYANDDSLFVQGTVSGPIVEDELFFRLSGTYRDTDGFWRNELKNDSSTI
                     DRFKNYDIDGRLLWEPNDRLSVDMKGRYGEVDASAITFNATFHLPIFAEGANAPAAFE
                     DVNDHEFVFQHNVDSDNNQEALELSVKADYEFENGYTLTGWMLYSDIQNDFIADGTSA
                     AFGFFQGNDACLDSTATLNAQGVILPPPQILGASPVGIIFTPDFSGSFFGPYTPTTCD
                     GIQEQVRDQDDFSFELRLASPVDQALRWMGGVYFLTIDRQVGISLNTDSGEKPIRGLL
                     QTSGPNRTEALVYDDFESDVFAVFGQLEYDITDAVELSFALRYDREERDVSNLVPTDV
                     YSTVIDLVPGDEDPLNPGLIGGAIPDKSKTFEEIQPKVALTWDLTEDFTIFGTWGKGF
                     KAGGFNNQGSQATVDGFINGLINGDVLGGPAFADILGVPLPQINDDFEEETSSAFEVG
                     FKSQLWDGRLSLEGAAYYVEVDDMQFFEFFVGTFGLLRVVSNLDEVEIYGFELAATAQ
                     LHDYVSVYAGANIIESEIKDNSARPDTVGNDAPYTPDYTFNVGAELNYPLSGDLNLFA
                     RVDAQFVGDTWFHTVQSGQRPTIFSPLFEISIFGEGSGPLGFADFENSKRDAYVTVDV
                     RAGIQGENWSVLGFAKNIGDENYLEEVIPAPEFGGAFDHPGARRRYGVEVSYRF"
     gene            complement(2700..3248)
                     /locus_tag="AC16PP_03365"
     CDS             complement(2700..3248)
                     /locus_tag="AC16PP_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6480692.1"
                     /translation="MAETAQTVPTLGVSSETMAAARQDIEYLRRWYAKATDLLGENTP
                     ESIAEGTKIYHRIFTADAYIRTSGATTQPFEATGPDAWAEVANTALESYVATQHLIGT
                     QIVDIHELTQDAGGTITSGRASMTSYLQAWHAGEENLWLFIGTYMDEVRFTPGVGWQI
                     HDMNLVQVSGEHRALGGPRPTP"
     gene            complement(3322..3807)
                     /locus_tag="AC16PP_03370"
     CDS             complement(3322..3807)
                     /locus_tag="AC16PP_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6480693.1"
                     /translation="MIRGIHHVAVSTPDLDRIVRFYCDVMGAEQVYEGGWEKGSQLID
                     GIVGLKDSSCRQAMLKLGNAYLEFFEYQTPEPRPGDPARPVCDHGYTHFAIDVTDIDS
                     EYERLKAAGVKFNCPPPDFEGGPIRATYGRDPDGNVIEIQEIKDTAHEFYLDAWSGGR
                     S"
     gene            3901..4707
                     /locus_tag="AC16PP_03375"
     CDS             3901..4707
                     /locus_tag="AC16PP_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6480694.1"
                     /translation="MATRHYDTIEVESEGQVDWLTLNRPESLNAINTPMVTELRDYFG
                     GLQENADTRIVVMRGAGRAFCAGLDIKQARSGEHDNPFGAGMGFQGYLAEVYIRMRRC
                     PQPIISLIQGPACGGGFAFVLASDIRIAGESARMNAAFIRIGLSACDMGVSYFLPRLV
                     GTSVASELMLTGRFIHAARALATGLVSEVVPDAELVDAARPYIDEMLTTSPMGLRLTK
                     EGLNMAVDAASLEAAMAIENRNQVLCSKTSDAREGMQAFLEKRAPEYTGA"
     gene            4712..5335
                     /locus_tag="AC16PP_03380"
     CDS             4712..5335
                     /locus_tag="AC16PP_03380"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF036807.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR6480695.1"
                     /translation="MALSVIGAGYGRTGTLSLKAALEQLGYLKCHHMIEVLQSPAQIE
                     GWMAAARGERVNWDELFAGYLASVDWPSCHFYRELADYYPQAKVVLTVRDPHAWYESM
                     NATTLRVIRRSMAEHPDRPSLGTELVVKGAFGGNIDSAAHAVEIFEAHTAEVMANIPS
                     ERLLVFDVREGWEPLCNFLQRDVPAAAFPRVNSREEFDGIFFGNRAD"
     gene            5364..6191
                     /locus_tag="AC16PP_03385"
     CDS             5364..6191
                     /locus_tag="AC16PP_03385"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6480696.1"
                     /translation="MSEKIVLVTGGAQGIGKGLARACLQRGASVVITNLDSEIAATTV
                     AELSEHGPIRAIRCDATDRNAVDALFDDVWATEGPVDLAFCNAGAGGMGQILETPMAD
                     VHHQFSTNLYSAIHLAQSYIPRLVEANRAGHVMFTGSENSLVLPRANADLAMGIYGGT
                     KHALLAVAEWLRYELRDTAVTVSLLMPGPVLTERLAATFDALADDPDNPEIRGRFPEH
                     VERTLRERFISTERCAEIALRGLAQGLFFIPTQAYIREDVDARYRELTAAFDALDLS"
     gene            complement(6255..6476)
                     /locus_tag="AC16PP_03390"
     CDS             complement(6255..6476)
                     /locus_tag="AC16PP_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480697.1"
                     /translation="MTIIEHPFTGAIYRMNDEGNILVTQGDRQGVFRPDGRWVSGEIR
                     EADPQMCVWVSNVPASPGESDWHVTSDKT"
     gene            complement(6473..>7803)
                     /locus_tag="AC16PP_03395"
     CDS             complement(6473..>7803)
                     /locus_tag="AC16PP_03395"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6480698.1"
                     /translation="NMVRSPGLTYQQLLDQDTHPVPDVLRLQSPKDFGDADISIERYI
                     SKEWHDREVERLWKRVWQFACREEDIPDPGDHYRYDIAGMSFLVVRTESGAIRTYPNA
                     CLHRGRMLKEFDGHASELRCPFHGFAWHLDGSLKDVPAKWDFPHIDDDQFGLPEIPTA
                     VWAGFVFINPDQQCAPFEDFISDMASQFERWDLGSLYKQAHVAKIMPANWKIAQEAFC
                     EAYHVNGTHPQILRSLGDINSQVDVWENCARVITPSLTPSPLLEYDLTDGDVVRAMLD
                     IPEGQPVPELPEGMSPRAWSAAMARETLRPDAGDRVDEYCDAELVDNLDYTVFPNFHP
                     WGAFNRIVYRFRPNGNDHRSAIMECIMLAPFQGERPPPAKIRWLAEDEPWSSALGFLG
                     RVFDQDAFNMPKVQQGLEATYKPGITLANYQESKVRWLHHKLTEWVEDGE"
BASE COUNT         1422 a         2534 c         2384 g         1463 t
ORIGIN      
        1 caggtccagc aggcgaatgc cctcgagagg cttcgacatc ttcgatccag ccctccccga
       61 ggttctaccc tagaagcggt agctcacctc gaccccgtaa cggcgcctgg cccccggatg
      121 atcgaacgcg ccgccgaact ccggcgccgg gatcacttcc tccagataat tctcgtcgcc
      181 gatgttcttg gcgaaaccca gaaccgacca gttctcgccc tgaatgccgg cacggacgtc
      241 caccgtcacg taggcgtcgc gcttggaatt ctcgaagtcc gcgaagccga gaggccccga
      301 cccttccccg aaaatcgata tctcgaatag cggggagaaa atcgtcggac gctgaccgct
      361 ctgaaccgtg tggaaccagg tatcacccac gaactgggcg tccacccggg caaagagatt
      421 caggtcgccg ctcaacgggt aattcagctc cgctccgacg ttgaaggtgt agtcgggtgt
      481 gtacggcgcg tcgttcccaa cggtgtcggg ccgcgccgag ttgtccttga tctcgctctc
      541 tatgatgttc gcacccgcgt agacgctgac gtagtcgtgc agctgggccg tggcggcgag
      601 ctcgaatccg tagatctcca cctcgtcgag gtttgagacc acccgcagga gcccgaaggt
      661 acccacgaag aattcgaaga actgcatgtc gtcaacctcg acgtaatagg ccgcgccctc
      721 gaggctcaat cggccatccc acagctggga cttgaagccc acctcgaagg cgctggaggt
      781 ctcctcctcg aaatcatcgt tgatctgcgg caacggcacg ccaagaatgt cggcaaatgc
      841 aggccctccg agcacgtctc cgttgatcag cccgttgatg aagccgtcga cggtggcctg
      901 gctgccctgg ttgttgaacc cgccggcctt gaagcccttg ccccaggtgc cgaagatcgt
      961 gaagtcctcg gtcaggtccc aggtcagcgc caccttgggc tggatctcct cgaacgtctt
     1021 gctcttgtcg ggaatcgcgc caccaatcag cccgggattg agcgggtctt cgtcacccgg
     1081 gaccaaatcg atcacggtgg aatacacatc cgtgggtacg aggttggaaa catcgcgctc
     1141 ctcacggtcg tagcgcaggg cgaaggagag ctccacggca tcggtgatgt cgtactcgag
     1201 ctggccgaag acggcgaaga cgtcgctttc gaagtcgtcg taaaccaggg cctcggtccg
     1261 gttcgggccg ctggtctgca gcaggccgcg gatcggcttc tcgccgctgt ccgtgttcag
     1321 ggaaatcccc acctgacggt cgatggtcag gaagtagacg ccgcccatcc agcgcagcgc
     1381 ctgatcgacg ggcgatgcga gacgcagctc gaagctgaaa tcatcctgat cgcgcacctg
     1441 ctcctggatg ccatcgcatg ttgtaggcgt atagggcccg aagaaggacc ctgagaaatc
     1501 cggcgtaaag attatgccca cggggctcgc accgagaatc tgcggtggtg gcagaatcac
     1561 gccttgggca ttcagggtcg ctgtggaatc cagacaggcg tcattgcctt ggaagaaccc
     1621 aaacgccgcc gacgtaccgt ccgcgatgaa gtcgttctga atatcgctgt agagcatcca
     1681 gcccgtgagc gtgtagccgt tctcgaactc gtagtcggcc ttcaccgaga gctccaacgc
     1741 ctcctggtta ttgtccgagt ccacgttgtg ctggaagacg aactcgtggt cgttgacgtc
     1801 ctcgaacgcc gccggcgcgt tggcgccctc ggcaaagatg ggcaggtgga aggtggcatt
     1861 gaaggtgatg gcggaggcgt ccacctcccc gtaacggccc ttcatgtcca ccgaaaggcg
     1921 atcgttgggc tcccagagca aacggccgtc gatgtcgtag ttcttgaaac gatcgatggt
     1981 cgacgagtcg ttctttaact cgttgcgcca gaagccgtcc gtgtcgcgat aggtgccgga
     2041 caggcggaag aagagctcgt cctccacgat ggggccactc accgtgccct ggacgaaaag
     2101 gctgtcatcg ttggcgtagc tcgtcttgat gctgccggta aactcctcgg tcggccgctc
     2161 cgtggtgacg atgaccgccc cggccgcggc gttacggccg tagatggcgc cctggggccc
     2221 tttgaacact tcgatctgct gcaggcccgg gtactcgcgg ttgaacgccg ccgggttggt
     2281 gtggaggatg ccgtcgacga tgaacgcaaa gctgttctcc gcgtcgcggg cgccgttgat
     2341 tccgcggatg ttgacctggg tgtcccctac ctcagcggtg tttaccagcg tcacacctgg
     2401 agtgagccgg atgaagtcct caacccgctc gaccccggcc ccctcgatgg tggactggcc
     2461 gatggccgtc acggttccgg gcacgtcctg aacggactcc gcccggcgcc gggcagtcac
     2521 caccagctcc tcgatcatcg ccccttcgcc ctcggcgaca gcgaactcag gcatgagcac
     2581 ggctaccgcc agcaccccgc cgatcaacgc gcttcttaag atttccatcg tttcttaccc
     2641 cctattgacc cttgtgattc tgaccctcgt cattcgggat caggaacacc gctcctgacc
     2701 tacggtgttg gacgcggccc cccgagcgca cgatgctccc ccgaaacctg aaccagattc
     2761 atgtcgtgta tctgccaccc cacgcccggc gtgaaccgaa cttcgtccat gtaggttccg
     2821 atgaacagcc acagattctc ctcacccgca tgccacgcct gcaggtagct ggtcatcgac
     2881 gcccggccgg acgtgatcgt cccgcccgca tcctgcgtca gctcgtggat gtccacgatc
     2941 tgcgtgccga tcaggtgctg cgttgccaca tagctctcca gagcggtgtt ggccacttcc
     3001 gcccaggcat caggccccgt cgcctcgaac ggctgggtgg tggcgcccga cgtcctaatg
     3061 taggcgtcag ccgtgaaaat ccggtgataa atcttggtgc cttcagcgat tgactccggc
     3121 gtgttctcac caagcaggtc ggttgccttg gcataccagc gcctcaagta ctcgatgtcc
     3181 tgccgcgcag ccgccatcgt ctcgctggac accccgagcg tcgggacggt ctgagccgtt
     3241 tccgccatca cacttcctcc catcaacgta gccgcgacaa tagcgcgaac gcccgcaact
     3301 tggctagcaa cgacatcgcc ttcaacttcg ccctcccgac caggcgtcca ggtagaactc
     3361 gtgcgccgta tccttgattt cctgaatctc gatgacgttg ccgtccggat cgcgtccgta
     3421 ggttgcgcga atcggaccgc cttcgaaatc ggggggcggg cagttgaact tgaccccggc
     3481 cgctttgagg cgctcgtact ccgaatcgat gtcggtgacg tcgattgcga aatgggtata
     3541 gccgtgatcg caaaccggtc tcgcaggatc tcccggtcgg ggctcgggcg tctgatactc
     3601 gaagaactcc aggtaggcgt tgcccagctt cagcatggcc tgacggcagg acgagtcttt
     3661 caagccgacg atcccgtcga tgagctggga acccttttcc caacccccct catagacctg
     3721 ctctgccccc atgacgtcac agtagaaacg aacgatcctg tccagatctg gggtcgacac
     3781 ggcgacgtgg tgaatgcctc gaatcaattt tcatcccctt caagtcgaga ttcacggaca
     3841 ttaacattga aaggcgaggt cggagaacat ccgcaccggc ctatcagcag ggagtcgagc
     3901 atggccacac gacactacga caccatcgag gtcgaaagcg aaggccaggt cgactggctg
     3961 acgctgaacc ggccggagtc tctgaacgcc ataaacacgc ccatggtgac tgagctgcgg
     4021 gattatttcg gcggtcttca ggagaacgcc gacactcgta tcgtggtgat gcggggcgcc
     4081 gggcgcgcct tctgcgccgg gctggacatc aagcaggcca gatccggcga gcatgacaac
     4141 cccttcgggg ccggcatggg cttccagggt tacctggccg aggtctacat ccgaatgcgc
     4201 cgctgccccc agcccatcat ctcgctgatc caggggccgg cctgcggcgg cggctttgct
     4261 ttcgtgctgg cctctgacat ccgcatcgcc ggcgagagcg cacgcatgaa cgccgccttc
     4321 attcgcatcg gcctttcggc ctgcgacatg ggcgtgagct actttctgcc acgactcgtc
     4381 ggcacctcgg tggcctcaga gttgatgctg accggccggt tcatccacgc tgcaagggcg
     4441 ctggccacgg ggctggtatc cgaggtggtc cccgacgcgg agctcgtgga cgcggccagg
     4501 ccgtacatcg acgagatgct gaccacatca cccatgggcc tgcgcctgac caaggagggc
     4561 ctcaacatgg cggtggatgc agccagcctg gaagccgcca tggccataga gaaccgtaac
     4621 caggtgctct gctcgaagac cagcgacgcc agggaaggta tgcaggcatt tctggagaaa
     4681 cgggcgcctg agtacaccgg cgcttaggcg ggtggctctg tctgtcatcg gcgccggtta
     4741 cggccggacg ggaaccctgt cgctgaaagc cgctttggaa cagctcgggt acctgaagtg
     4801 ccaccacatg atcgaggtgc tgcaaagccc ggcgcagatt gagggctgga tggccgcggc
     4861 gcgcggcgag cgggtcaatt gggacgagct cttcgccggc tacctggcca gcgtcgactg
     4921 gccctcctgc cacttctacc gggagctcgc cgactactac ccgcaggcca aggtggtgct
     4981 gaccgtgcgc gacccccatg catggtacga gagcatgaac gcgacgacgc tacgggtcat
     5041 tcgtcgttcg atggccgagc acccggaccg ccccagcctg ggcaccgagc tggttgtgaa
     5101 aggcgctttc ggcggaaaca tcgacagcgc cgcccacgcc gtcgagatct tcgaagccca
     5161 caccgccgag gtgatggcca acattccctc cgagcggctg ctggtgttcg acgtccgcga
     5221 aggatgggag ccactgtgca actttctgca gcgcgacgtg cccgccgctg ccttcccacg
     5281 ggtcaattcc cgggaagagt tcgacggcat tttcttcggc aatcgcgccg attgactgca
     5341 accgaggtat gaaaatggaa tcgttgagcg aaaaaatcgt cctggtcacc ggcggcgccc
     5401 agggtatcgg caaaggcctg gcgcgcgcct gcctgcaacg cggtgcgagc gtggtgatca
     5461 cgaacctgga ctccgagatc gcggcgacga cggtagcgga gctgtccgag cacggcccca
     5521 ttcgggccat tcgctgcgat gccaccgacc gaaatgccgt ggatgccctc ttcgacgacg
     5581 tctgggccac cgagggcccc gtcgacctgg cgttctgcaa cgccggcgca gggggcatgg
     5641 gccagattct cgagaccccc atggcggacg tgcaccacca gttctccacc aatctctact
     5701 cggccattca cctggcgcag agctacatcc cacgcctggt cgaggcgaac cgcgccggcc
     5761 acgtcatgtt caccgggtcg gagaactccc tcgtcctgcc gcgggccaat gcggatctgg
     5821 ccatgggtat atatggaggg accaagcacg ccctgctggc cgtcgcggaa tggcttcgct
     5881 acgagttgag ggacacggcc gtcaccgtca gcctgctcat gccggggccg gtgctgaccg
     5941 aacggctcgc ggccaccttc gacgccctgg ccgacgaccc ggacaacccg gagatccggg
     6001 gccggtttcc cgagcacgtg gagcgcaccc tgcgggagcg tttcatctca accgagagat
     6061 gcgcggagat cgctttgcgc ggcctcgctc agggactctt cttcatcccg acccaggcgt
     6121 acattcgaga ggacgtggac gcacgctacc gcgagctcac tgccgcgttc gacgccctgg
     6181 atctgtcctg atccgccctc ttctcacccg ccatgcgcct gcgccacggg gcgcggcggc
     6241 gattgctccg gcggtcaggt cttgtccgag gtaacgtgcc agtccgactc cccggggctg
     6301 gccggcacgt tggacaccca gacacacatc tgcggatcgg cctcgcggat ctccccggag
     6361 acccatcgtc cgtcaggccg gaaaacaccc tggcgatcgc cttgcgtgac caggatgtta
     6421 ccttcgtcgt tcatccggta gatggcaccg gtgaacgggt gctcgatgat ggtcactcgc
     6481 cgtcctccac ccactcggtg agcttatggt gcagccagcg caccttggac tcctggtagt
     6541 tcgcaagggt gatgccgggc ttgtaggtcg cctcgaggcc ctgctgcacc ttgggcatgt
     6601 tgaacgcgtc ctgatcgaag acccggccga gaaagccgag cgccgatgac cagggctcgt
     6661 cttcggccag ccagcggatc ttcgccggag gcggtcgttc tccctggaac ggggcgagca
     6721 tgatgcactc catgatggcc gacctgtggt cgttgccgtt cggccgaaag cgatagacga
     6781 tgcggttgaa cgcgccccac gggtgaaagt tgggaaacac cgtgtagtcc aggttgtcta
     6841 ccagctccgc atcgcagtac tcgtctaccc tgtcgcctgc gtccggacgc aaggtctccc
     6901 gagccatggc cgcgctccag gccctcggcg acataccttc cggcagctcc gggacgggct
     6961 ggccttccgg gatatcgagc atggcgcgca ccacgtcgcc gtcggtcagg tcatactcga
     7021 gcaaggggct gggagtcagg gagggcgtga tgacgcgggc gcagttctcc cagacgtcca
     7081 cctggctgtt gatgtcgccc aggctcctca agatctgcgg atgcgttccg ttgacgtggt
     7141 atgcctcgca gaaagcttcc tgggcgatct tccagttagc cggcatgatc ttggccacgt
     7201 gggcctgctt gtaaaggctg cccaggtccc agcgctcgaa ctggctggcc atgtcgctga
     7261 tgaaatcctc gaacggcgcg cattgctggt ccgggttgat gaagacgaaa ccggcccaga
     7321 ccgccgtcgg tatctccggc aggccgaact gatcgtcatc gatgtgggga aagtcccatt
     7381 tcgcgggaac gtccttgagc gacccatcca gatgccaggc gaagccgtga aacgggcagc
     7441 gcagttccga ggcatggcca tcgaactcct tgagcatgcg cccccggtgc aggcaggcat
     7501 tgggatacgt cctgatggcg ccgctttccg tccggaccac gagaaacgac attcctgcga
     7561 tgtcgtaacg atagtgatcg ccggggtcgg gaatgtcttc ctcccgacaa gcgaactgcc
     7621 acacgcgctt ccacagccgc tccacctcac ggtcatgcca ctctttggag atatatcgct
     7681 cgattgagat gtcggcatcg ccgaaatcct tcggcgactg cagcctcagc acgtcgggaa
     7741 ccgggtgggt atcctgatcg agcagctgct gatatgtgag gcccggcgag cggaccatgt
     7801 tgt
//
LOCUS       DBNHKV010000105         3625 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_865436, whole genome shotgun sequence.
ACCESSION   DBNHKV010000105 DBNHKV010000000
VERSION     DBNHKV010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_865436"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1473)
                     /gene="hldE"
                     /locus_tag="AC16PP_03400"
     CDS             complement(49..1473)
                     /gene="hldE"
                     /locus_tag="AC16PP_03400"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HIR6480699.1"
                     /translation="MNLPSLQGVHVLVVGDLMLDRYWIGPAERMSQEAPVPVVRVERV
                     EEHPGGAANVALNVASLGARCTLIGYVGKDDAAEALVDRLTAAGVACDLVVVDGWSTV
                     VKLRVVSQNQQLIRTDFESALIPGEDHRALLREKVIARLLDASAMVLQDYDKGVIEDP
                     ETLIGSAIAAGVPVVVDPKRKPFARYAGAAILKPNAKELEAALGSWDDDTDLVSLSAA
                     ACRTHRLGAMVITRGAGGMTVVAADGQHRHIPALPADVYDVTGAGDTAAAALAVTTSL
                     GWDADRGAQVANVASGLVVAKAGTAAITGPELALALAGRHRVDRGALRRAQLEDAVAQ
                     ARAAGERIVFTNGCFDILHAGHVAYLEEARALGDRLVVAVNDDDSVARLKGPGRPVNS
                     LERRLRVLAGLASVDWVVGFSEDTPEALLELLKPDVLVKGGDYGPSEVVGADQVIAQG
                     GEVRVLGLVEDCSTTAIVDRIRGA"
     gene            1537..2454
                     /gene="lpxL"
                     /locus_tag="AC16PP_03405"
     CDS             1537..2454
                     /gene="lpxL"
                     /locus_tag="AC16PP_03405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HIR6480700.1"
                     /translation="MSRRRKSRPRMYSPRVWGTWLIVAIGWLLARLPISAIGGLGRWL
                     GRIAYRLARSRRHIAEVNLRLCFPDLGEAARREMIRGVFQHVAVGALETMVVWLDPDR
                     DLGDRVTIEGLEHLRAAQAEGKGVLLLGGHFTSLDVMANAVSRAVDLDVIYRANKNPV
                     WEWLQVRGRSRYCDGVIERSDVRQTIARLKAGRTIWYAPDQDYGPKHSVFAPFFGAPA
                     ASITATSRLARISGSPVIFASHFRDWNTMTWHVTFSEPLDGFPTSDEVADAARVNQLI
                     ENAIRAHPDQYLWIHRRFKTRPPGEGKLY"
     gene            complement(2448..>3625)
                     /locus_tag="AC16PP_03410"
     CDS             complement(2448..>3625)
                     /locus_tag="AC16PP_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007939655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase family protein"
                     /protein_id="HIR6480701.1"
                     /translation="VYGAMTEYLGFEMLDGEYKVMGMAPYGDPDLYDLGRLIRRARDG
                     FEVDVSYVNTVGLRRHKESGVGFYFSDKLINWLGPRRQGEVADEPYIHYAASMQKLFE
                     EWCLELMERYLGDVLRETGRLAFAGGGALNVKLNQRIVALDHVRELFVQPAAGDSGTS
                     LGAATYVAASEGQRLSPMTHVFLGPRYDNDDCLQALRARRESLHWRRLDDVPARAAEL
                     LAQGHPLAWFQGRMEFGPRALGARSILGCPSTPGIADRINEQIKFRERWRPFCPSVLD
                     RVAAEIIQTEHPAPFMTITFDVAQEWKSRIPEVVHEDGTARVQIVDPDSNPRYYALLE
                     TLETLTGNAVVLNTSLNRRGEPMVCSPEDALNMFCGSDLQFLVLEDFLVSKTDEEAQ"
BASE COUNT          620 a         1264 c         1137 g          604 t
ORIGIN      
        1 tgcccggctt gaagccggcc ccgcgccggc acatcccggg ctcggcgcct aagcgccccg
       61 gatccgatcg acgatggcag tggttgagca atcctccacc agaccgagca cgcgcacctc
      121 gccgccctgg gcgatgacct gatccgcgcc aaccacctcg ctgggcccgt agtcgccccc
      181 cttgaccagg acgtccggtt tcaacagctc cagcagggct tcgggcgtat cctccgagaa
      241 cccgacgacc cagtcgaccg acgcgagccc ggccagcaca cgcaagcgtc gttcgagaga
      301 attcaccggc cgacctgggc ctttcagacg cgccaccgag tcatcgtcgt tgacggccac
      361 caccaggcga tcgcccagcg cccgggcttc ctccagatac gcgacgtggc ccgcgtgaag
      421 gatgtcgaag cagccgttgg tgaagacgat ccgctcgccg gccgctcgcg cctgagcgac
      481 cgcgtcctcc agctgcgccc tgcgcagagc accgcggtcc acccgatgac gcccggccag
      541 cgcaagcgca agctcgggcc ccgtgatcgc cgccgttccc gccttggcca ccaccagccc
      601 gctcgccacg ttcgccacct gggcaccgcg gtccgcatcc cagcccaggc tggtggtcac
      661 ggcgagcgcg gcagcagcgg tatcccccgc gcccgtcacg tcgtaaacgt ccgccggcaa
      721 ggccgggatg tgccggtgct gcccgtcggc cgcaacgacc gtcatcccgc cggcaccccg
      781 ggtgatgacc atggcaccca ggcgatgggt tctgcaggcc gccgccgaca atgagacgag
      841 atccgtgtcg tcgtcccacg agccgagcgc cgcctcgagc tcctttgcat tgggcttgag
      901 gatggcggct cccgcatagc gggcaaaggg cttgcgttta gggtccacca ccaccggcac
      961 gcccgccgcg atggcggagc cgatcagcgt ttcggggtcc tcgatcacgc ccttgtcgta
     1021 atcctgaagc accatggccg aagcatccaa cagccgggca atgacctttt cgcgcagcag
     1081 cgcgcgatgg tcctcgcccg ggatcagagc gctttcgaaa tcggtgcgga tgagctgctg
     1141 gttctggctg accacccgca gcttgaccac ggtggaccaa ccgtctacca cgaccaggtc
     1201 gcaagcgacc ccggctgccg tgagcctgtc gacgagggct tcggccgcgt cgtctttacc
     1261 tacgtaacca atgagggtgc accgcgcgcc gagactggcg acgttgaggg ccacgttggc
     1321 cgcgcctccg ggatgctcct ctacccgctc caccctgacc accgggaccg gggcttcctg
     1381 agacatccgc tcggcgggac caatccagta gcggtccagc atgagatcgc cgaccaccaa
     1441 gacgtgaacc ccttgcagcg acggcagatt catggggcgt ggatggtatc acagggcctc
     1501 cccgttacaa ttcgcctacc ccgccggtgg aacctcatga gcaggcgcag aaagagccga
     1561 ccccgaatgt actctcctcg cgtctggggg acctggctca tcgttgccat cggctggctg
     1621 ctcgcgcgtc tgcccatatc tgcgatcggg gggctcgggc gatggctggg ccgtatcgcc
     1681 taccgcctcg cccggtcacg acgacacatc gccgaggtca acctcaggct gtgttttccc
     1741 gatctgggag aagccgcgag acgggagatg atccggggcg tgtttcagca cgttgcggtg
     1801 ggcgccctgg agaccatggt ggtctggctg gaccctgacc gcgacctcgg cgaccgagtg
     1861 accatcgaag gcctcgagca tctgcgggcc gcgcaggccg aagggaaggg cgtgctgctt
     1921 ctgggcggcc acttcacgtc cctggacgtg atggccaatg cggtttcccg cgccgtagac
     1981 ctcgatgtca tataccgcgc caacaagaat cccgtctggg agtggctgca ggttcgaggg
     2041 cggagccgct actgcgacgg cgtcatcgag cgctccgacg tccgccagac gatcgcgcgt
     2101 ctcaaggcgg ggcgcacgat ctggtacgcg ccggaccagg actacgggcc caagcactcc
     2161 gtctttgcgc ctttcttcgg cgcgcccgcc gcctccatca ccgccacgtc tcgcctcgcg
     2221 cggatctccg gctcgcccgt gatctttgcc agccattttc gcgactggaa cacgatgacc
     2281 tggcacgtca cgttcagcga gccgctcgat gggttcccga ccagcgacga ggtggccgac
     2341 gcggcacggg tcaaccaatt gatcgagaac gcgatccgcg cgcacccgga tcagtacctc
     2401 tggattcatc gccggttcaa gacgcgtccc ccgggcgagg gaaagctcta ctgagcttcc
     2461 tcgtccgtct tgctgacgag aaaatcctcg agcacgagaa actgaagatc ggacccgcag
     2521 aacatgttca gcgcgtcttc cggcgagcag accatgggtt ctccccgccg gttgagagac
     2581 gtgttgagca ccaccgcgtt gcccgtgagc gtttccaggg tctcgagcaa cgcgtagtac
     2641 cggggattgc tgtccggatc cacgatctgg acacgggcgg tcccgtcctc gtgaacgact
     2701 tccgggatcc gcgatttcca ttcctgcgcc acatcgaagg taatggtcat gaaaggcgcg
     2761 ggatgctcgg tctggatgat ttccgctgcg acccgatcga gcacgctggg gcagaaaggc
     2821 cgccacctct cgcggaactt gatctgctcg ttgatgcgat cggcaatccc gggcgtgctg
     2881 ggacaaccca gaatgctgcg ggcgcccagg gcccgggggc cgaactccat gcggccctgg
     2941 aaccacgcca gcggatgccc ctgggcaaga agttccgccg cccgagcggg cacgtcgtcc
     3001 aggcgccgcc agtgaaggga ctcgcgacgg gctcgaaggg cctgaaggca atcgtcgttg
     3061 tcgtaacgcg gaccgagaaa gacgtgggtc atgggggata ggcgttgacc ttcgctggct
     3121 gccacgtagg tggcggcccc cagtgaagta cccgagtctc cggccgccgg ctggacgaac
     3181 agctcacgaa cgtgatccag ggcaacgatt cgctggttca gcttcacgtt gagggctccg
     3241 ccgcctgcaa aagccagccg cccggtctcc cgcagcacgt ccccgaggta tctctccatg
     3301 agttccaggc accactcctc gaagagcttc tgcatggatg cggcgtagtg gatgtagggc
     3361 tcgtcagcca cctccccttg ccggcgtggt ccgagccagt tgatgagctt gtcactgaag
     3421 tagaagccaa cgcccgactc cttgtgacgt cgcaggccca ccgtgttcac gtagctcacg
     3481 tccacctcga agccgtcgcg tgctcggcgg atcaggcgcc cgagatcgta taggtcggga
     3541 tctccgtagg gcgccatgcc catcaccttg tactcaccgt cgagcatctc gaagccgagg
     3601 tattcggtca tcgcgccgta gacgc
//
LOCUS       DBNHKV010000106         6213 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_582043, whole genome shotgun sequence.
ACCESSION   DBNHKV010000106 DBNHKV010000000
VERSION     DBNHKV010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6213)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_582043"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..409)
                     /locus_tag="AC16PP_03415"
     CDS             complement(122..409)
                     /locus_tag="AC16PP_03415"
                     /inference="COORDINATES: protein motif:HMM:NF015735.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR6480702.1"
                     /translation="MLYVMICEDKPDSEALRLANRADHLEWVGGKTAMIRLAGPMLSD
                     DGEHMRGSLFIIQAESADAVRQFNGEDPYTRAGLWGKVTIRPFRQVLPADS"
     gene            complement(454..1392)
                     /locus_tag="AC16PP_03420"
     CDS             complement(454..1392)
                     /locus_tag="AC16PP_03420"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6480703.1"
                     /translation="MPLAPEYQSFLEQLAQVPGPPLHELSPADARAAYRLLRPRDEQI
                     TVAAVNELAAPGPAGSIALRVYRPQGDGPFPMLVNFHGGGWVFGDLDTADTVCRNLCR
                     NTGCLVVSVDYRLAPEHPYPAAIDDAYAATCWLAEHASELEGNGRLAVAGESAGGNLA
                     AAVSLRAREEGGPAIDYQLLFYPVIDADFNRASYEENRSGYLLETATMIWFWDHYVPD
                     RSRRSQAYASPIRADDLGRLPPALILTAEFDPLRDEGNAFAEALKAAGTRAELICCAG
                     LIHDFCGTAHLFESSRKPFEDACAALRRAMSGTPES"
     gene            1453..2043
                     /locus_tag="AC16PP_03425"
     CDS             1453..2043
                     /locus_tag="AC16PP_03425"
                     /inference="COORDINATES: protein motif:HMM:NF009205.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIR6480704.1"
                     /translation="MTRLDGVEEAAEVAKQFQFLENTLLKNRTLTLFGEINKDVAQRT
                     AEKLLALAYESDDPIVLYIGSPGGHVESGDTIFDMIRYIKPNVKAVGTGWVGSIATHI
                     YLAPDREDRFCLPNTRFLIHQPAGGFGGSASDVEIHAREIVRTRQRINAIIAEQTGQP
                     LERVEEDTDRDYWMSAEESVEYGIVGRIISSIDELG"
     gene            complement(2063..2800)
                     /locus_tag="AC16PP_03430"
     CDS             complement(2063..2800)
                     /locus_tag="AC16PP_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6480705.1"
                     /translation="MSELVLREDREGLAILTLNRPEALNALSPSLFVELRRHVDDIAQ
                     DVDNVGCVLLRGEGRSFSAGNDLKAIQAGERAPGPHFQAETLDAIEGLPQPVIAAVQG
                     HCYTGSLELALACDLIITARGAKIADTHGKWAMSPTWGMSQRLPRRVGLARAKELMYS
                     GRVIDGEEAAAIGLAVRCVPDEELLEAAVAMARTFLENSWFTLRADKMLVNGGLNHTL
                     ADGLAFERSNSPGRGPDLEERLERFGK"
     gene            complement(2837..4036)
                     /locus_tag="AC16PP_03435"
     CDS             complement(2837..4036)
                     /locus_tag="AC16PP_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6480706.1"
                     /translation="MDISFSAEHERFRDEVRVFLDGALDDELREAQRFCPGIFLDYEH
                     NIRWHRILHAKGWVAPAWPREYGGTGWDLTRRYIWSTETTLAGAPSLAPMGLGMCGPM
                     LIGYGTEAQKAYYLPRILSGEDYWCQGYSEPGSGSDLASLKLRADGDGDHYVLNGTKI
                     WTTHAHYANRMFCLVRTDDGGRPQQGITFLLLDMDTPGISVEPIVFASGTHEVNQVFF
                     DDVRVPKANRVGEENDGWTVAKYLLEFERGGGGSAAYRTALTRVRKLAGQVPADGASL
                     AEDPDFKRALDETDVKLQAIQYTEFRIMAALSRGQNPGPESSIMKNLGADIGQRLTEL
                     TVEAMGQYIAPHQPEARQVGSNVPVVGPADGATAMPRYFNLRASSIAGGTNEVQKNIV
                     AKLVLGL"
     gene            complement(4036..4575)
                     /locus_tag="AC16PP_03440"
     CDS             complement(4036..4575)
                     /locus_tag="AC16PP_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HIR6480707.1"
                     /translation="MNVWLFGYGSLIWRPEIAYVERRPARLHGWARRFWQGSHDHRGM
                     PRQPGRVVTLIEEEGGHCDGMAYLVDRGDVEAIFAALDHREKNGYRRFDVELHFSRYN
                     PAPATTGVVYIAPPGNHAFLGPAPLAEMARQISECAGPSGRNVDYLLDLAEALRQLDA
                     QDAHVFELEAAVRHVSGSR"
     gene            complement(4592..5773)
                     /locus_tag="AC16PP_03445"
     CDS             complement(4592..5773)
                     /locus_tag="AC16PP_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR6480708.1"
                     /translation="MDGRQQAIVNRIDELADELLAVSHAIHANPELAFEEHDACALLA
                     STARRFDLPVETGVFTLETAFQGSFGGRTSGPTVAILAEYDALPGIGHACGHNLIATS
                     ALGAALGLKAVDDELPGTVRLIGTPAEERGGGKELMARAGAFDGVDAAMMIHPAGVNL
                     ATMPCICVAEVEVIYHGRSAHASAMPHKGINALDGLLLAYQAISNLRQHIRDHERIHG
                     IVTEGGLAPNIVPDRAAGAFYVRAANEQDLARLKPRVQACFEAGAKGSGCELEVNWAN
                     VDYLDLNTNWPLANRFQAHGERLGREFLKPASLGGAGSTDMGNVSYRVPSIHPMLACA
                     PPNVVIHNPEFAKWARSEQGDAAAIDGAKALALTAADYLLDGSLQAEVNKAFAISKGL
                     A"
     gene            5875..>6213
                     /locus_tag="AC16PP_03450"
     CDS             5875..>6213
                     /locus_tag="AC16PP_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6480709.1"
                     /translation="MAYDYELLDVDIDGRVATVTVSNPPINLITVALYRELASLGAEL
                     RADPNLTVVVMKSADPDFFLAHFDVETILSFPVDGEAQRSAELSEYHLMCERFRTMDK
                     VTIAQIEGRVG"
BASE COUNT         1036 a         1995 c         2068 g         1114 t
ORIGIN      
        1 cgtgcgtccg gcgaccatca cgtggagacc ctcgccggca aatcgtttgc acagctgggc
       61 gccgagcccg aggtcgggcc cgacgccgag gatgacggct tttctcatgg tctgggtcgc
      121 ctcatgaatc tgctggcagg acctgtctga agggccggat ggtcaccttg ccccagagcc
      181 ccgcgcgcgt gtagggatcc tccccattga actgtctcac cgcatcggct gactcggcct
      241 ggatgatgaa cagggatccg cgcatgtgct caccgtcgtc gctcagcatg gggccggcga
      301 gccgtatcat cgccgtcttc ccgcccaccc attccaaatg gtcggcccgg ttcgccagac
      361 gcagcgcctc gctgtccggt ttgtcttcgc agatcatcac gtaaagcatg cttcttcctc
      421 ggtcaggctt cagttttgcg ggggtgttcg gcttcagctt tcgggcgtgc cgctcatggc
      481 ccggcgcagg gcggcacagg catcctcgaa cggctttcgg ctcgactcga aaagatgcgc
      541 ggtgccgcag aagtcgtgaa tcaggccggc gcagcagatc agctctgccc gcgtgcccgc
      601 ggctttcaac gcctcggcga aagcgttgcc ttcgtcccgg agcgggtcga actccgcggt
      661 caggatcagc gctggcggca ggcggcccag atcgtctgcc cgaatgggcg aagcgtacgc
      721 ctgagaacgg cgtgacctat cgggcacgta gtggtcccag aaccagatca tcgttgccgt
      781 ctcgagcagg tagccgctcc ggttctcctc gtaggaggcc cggttgaagt ccgcatcgat
      841 aacgggatag aagagcagct gatagtcgat ggccggaccg ccttcctccc gtgcgcgcag
      901 gctcacggcg gcggccagat ttccgcccgc gctttcgccg gccactgcca ggcggccgtt
      961 gccctccagc tcgctcgcgt gttccgccag ccagcaagtg gcggcgtacg cgtcgtcgat
     1021 ggccgcgggg tagggatgct cgggcgccag ccggtaatcc acggagacga ccaggcaccc
     1081 cgtgtttcgg cagagattgc ggcagacggt atcggcggta tcgaggtcgc cgaataccca
     1141 gcccccgccg tgaaagttca ccagcatggg aaacgggcca tctccctggg gacggtagac
     1201 gcgcagcgca atggatccgg ctggcccggg cgcggccagc tcgttcaccg cggcgacggt
     1261 gatctgctcg tccctcggcc gcaagaggcg gtaagcggcg cgggcatcgg cgggcgacag
     1321 ttcgtgcagg ggagggcccg gcacctgggc gagctgctcg agaaaactct ggtattccgg
     1381 cgctaacggc attcgtatcc cccgtgtaga atgcgcccca agtttaaacc gaaagatatc
     1441 tggagacacc ggatgacgag gcttgatggc gtggaagaag cggcggaggt agccaagcag
     1501 tttcagtttc tggagaacac gctgctcaag aaccgaacct tgaccctgtt cggggagatc
     1561 aacaaggacg tcgcccagcg cacggcggag aaacttctgg cgctggcata cgagtcggac
     1621 gaccccatcg tgctctacat cggatccccc ggtgggcacg tggagtcggg cgatacgatt
     1681 ttcgacatga tccggtatat caagccgaac gtgaaagccg tgggcaccgg ctgggtcggg
     1741 agcatcgcaa cgcacatcta cctcgcgccc gaccgcgagg accgcttctg cctccccaac
     1801 acccggtttc tcatccatca gcctgcgggc ggctttggcg ggagcgcatc ggacgtggag
     1861 atccacgccc gcgagatcgt cagaacccgt cagcgcatca acgccatcat cgcggagcag
     1921 acgggccagc ccctcgaacg ggtggaagag gacaccgacc gcgactactg gatgagcgcc
     1981 gaagagtcgg tggagtacgg catcgtgggt cgaatcatct cgagcatcga cgagctcggc
     2041 tgacgacggg ccaccaccgg tctcacttgc cgaagcgctc gagccgctcc tcgagatcgg
     2101 gccccctgcc tgggctgttg cttcgttcga acgcaaggcc gtcggctagc gtgtggttca
     2161 acccgccgtt caccagcatc ttgtcggctc gcagcgtgaa ccaggagttc tccaggaacg
     2221 tgcgggccat ggcgacggct gcctcgagca gttcctcatc cggcacgcag cgcaccgcta
     2281 gaccgatggc ggccgcctcc tcaccgtcga tgacgcggcc gctgtacatg agctccttgg
     2341 cgcgagccag gccgactctt ctaggcaggc gctggctcat cccccaggtt ggtgacattg
     2401 cccacttgcc gtgggtgtcc gcaatcttcg cgccgcgggc ggtgatgatg agatcgcagg
     2461 ccagcgcaag ctccagcgag cccgtgtagc aatgcccctg aacggccgcg atgaccggct
     2521 gtggcagccc ctcgatggca tccagcgttt cggcctggaa gtgcgggccc ggcgcgcgct
     2581 ccccggcctg aatggctttc aggtcgttcc cggcggagaa ggaccttcct tcgccgcgca
     2641 gcagcacgca gcccacgttg tcgacatctt gcgctatgtc gtccacgtgg cggcgcagct
     2701 ccacaaacag gctggggctc agcgcgttca aggcttccgg tctgttcagg gtgaggattg
     2761 ccagaccttc gcggtcctcc cgcagtacga gttcgctcat gtcacttccc ttgagagttg
     2821 tcggccagcg ccccgttcac aggccgagga ccagcttggc gacgatgttc ttctgcacct
     2881 cgttggtgcc tccagcgatg gagctggcgc gcaggttgaa gtagcgcggc atggcggttg
     2941 cgccgtccgc gggtcctacc acggggacgt tgctgcccac ctgccgggcc tcgggctgat
     3001 ggggcgcgat gtactggccc atggcttcga cggtcagctc ggtgaggcgc tggccgatgt
     3061 cggcgcccag gttcttcatt atggaggatt cgggcccggg gttctggccc cgggagagcg
     3121 cggccatgat gcgaaactcg gtgtattgaa tggcctgcag ctttacgtcg gtttcgtcca
     3181 gcgcccgctt gaaatccggg tcttcggcca gggaagcgcc atcggcggga acctggccgg
     3241 ccagtttgcg aacccgggtc agggccgtcc ggtaggcggc gctgccgccg ccacctcgtt
     3301 cgaactcgag caggtacttg gccacggtcc agccgtcgtt ctcctcaccg acacggttgg
     3361 ccttgggaac cctgacatcg tcgaagaaca cctgattgac ctcgtgagtg cccgatgcga
     3421 acacgatggg ctccacgctg atgcccggcg tgtccatgtc gagaagcaga aacgtgatcc
     3481 cctgctgggg acgtcccccg tcgtcggttc tgacgaggca gaacatgcga ttggcgtaat
     3541 gcgcgtgggt ggtccagatc ttggttccgt tcaggacgta gtggtcgccg tcgccgtctg
     3601 cccggagctt gagggaggcg agatccgacc cggaacccgg ctccgagtag ccctggcacc
     3661 agtagtcctc gccggagaga atccgcggga ggtaataagc tttctgggct tcggttccgt
     3721 agccgatcag catcgggccg cacatgccaa ggcccatcgg cgcgagcgag ggtgcgccgg
     3781 caagggtggt ctccgtggac cagatgtacc ggcgcgtcag gtcccagccg gtgcccccgt
     3841 attcccgggg ccacgcgggg gcgacccagc ccttggcgtg caggatgcgg tgccagcgga
     3901 tgttgtgctc gtaatccagg aagatgccgg ggcagaagcg ctgggcttcg cgcagctcgt
     3961 cgtccagggc cccgtcgaga aaaacccgaa cctcgtcgcg gaagcgttcg tgctccgcgc
     4021 tgaaggaaat gtccattatc tgctcccgga tacgtgccgg acggccgctt ccaactcgaa
     4081 cacatgggcg tcctgggcgt caagctgacg cagcgcttcg gccaggtcga gcaggtagtc
     4141 gacgttgcgg ccgctcggcc ccgcgcactc gctgatctgg cgagccatct ccgcaagcgg
     4201 ggcgggtccc aggaaagcgt gattgcccgg gggcgcgatg tagaccactc ccgtggtggc
     4261 cggcgcagga ttgtagcgag agaaatgcag ttccacgtcg aagcgccgat agccgttctt
     4321 ctctcgatga tccagcgccg cgaatatggc ttcgacatcg cccctgtcca cgagataggc
     4381 catgccgtcg caatgaccgc cttcctcctc gatgagggtc accaccctgc cgggctggcg
     4441 gggcatgccg cggtggtcgt gggagccctg ccagaagcgg cgcgcccagc cgtggaggcg
     4501 ggcggggcgg cgctccacgt aagcgatctc cgggcgccag atgagggatc cgtagccgaa
     4561 cagccagacg ttcatcaggc cgggcgggcg gtcaggccag gcccttcgag atcgcgaacg
     4621 ctttgttcac ctccgcctgc agcgagccat ccaggaggta gtcggcggcg gtcagagcca
     4681 gggctttcgc gccgtcgatg gccgcggcgt cgccctgctc ggaacgagcc cacttggcga
     4741 actccgggtt gtggatgacg acgttgggtg gagcacaggc cagcatcgga tgtatggatg
     4801 gtacccggta gctgacgttc cccatgtcgg tgctgcccgc gccgccgagg gaagcgggtt
     4861 tgagaaactc gcgccccaga cgctcgccgt gggcctggaa gcggttggca agaggccagt
     4921 tggtgttcag gtccaggtag tcgacgttcg cccagttgac ctctaattcg cagccgctcc
     4981 cttttgcgcc tgcctcgaaa catgcctgca cgcgcggctt caggcgggca agatcctgtt
     5041 cgttggcggc tcgcacgtag aaggccccgg cggcccggtc ggggacgatg ttcggtgcca
     5101 ggcctccttc ggtgacgatg ccgtggatgc gctcgtgatc gcgtatgtgc tggcgcaggt
     5161 tggatatggc ctggtaggcg agcaggaggc cgtcgagggc attgatgccc ttgtgaggca
     5221 tcgccgaggc gtgggcagag cggccgtgat agatcacctc cacctcggca acgcagatgc
     5281 agggcatggt cgcaagattc acccccgcgg gatggatcat catcgccgcg tccacgccgt
     5341 cgaatgcgcc cgcccgcgcc atcagttcct ttccgccccc gcgttcctcg gcaggggtcc
     5401 cgatgagtcg gacggtcccg ggcagctcat catccaccgc cttgagcccc agagcggcac
     5461 cgagagcgga tgtcgcgatg aggttgtggc cgcacgcgtg gccgatcccg ggcagagcat
     5521 cgtactccgc gagaatcgcc acggtgggcc ccgacgtgcg cccgccaaag cttccctgga
     5581 aggcggtttc cagggtgaaa acgcccgttt ccaccggaag atcgaaccgc ctggccgtgc
     5641 ttgccagcag cgcgcaggca tcgtgctcct cgaaagcgag ctccggattg gcgtgaatgg
     5701 catgggacac ggcgagcagc tcgtcggcga gctcgtcgat tcggttgacg atggcctgct
     5761 gtcttccgtc catattccgg tcaggtcgat ataatgccct cgagtatggg gcatcgtgcg
     5821 gcacgcctcc agaggaatgc cgcggcgctt tgatcaccag gaagggagat atccgtggct
     5881 tacgattacg agctgctcga cgtggacatc gatgggcggg tagcgacggt aaccgtcagc
     5941 aatccgccca tcaacctcat caccgtggcc ctctaccgcg agcttgcgag tctcggcgcc
     6001 gagcttcggg ccgacccgaa cctaacggtg gtggtgatga agagtgcgga cccggacttc
     6061 tttctcgcgc atttcgacgt ggaaaccatt ctttcgttcc ccgtggacgg tgaggcccaa
     6121 cgctccgccg agctttcgga gtaccacctc atgtgcgagc ggttccgcac catggacaag
     6181 gtgaccatcg cgcagatcga agggcgcgtc ggc
//
LOCUS       DBNHKV010000107         5373 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_653639, whole genome shotgun sequence.
ACCESSION   DBNHKV010000107 DBNHKV010000000
VERSION     DBNHKV010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_653639"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="AC16PP_03455"
     CDS             complement(<1..738)
                     /locus_tag="AC16PP_03455"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HIR6480710.1"
                     /translation="MADYVLTFSCPDRPGLVHAVSSWLVDLNGNILDSDQFRDDTTER
                     FFMRVHFEAPDDLPALNQTFIPTAGAMNMDWQIWPTARRPRVLMLVSKIDHCLADLLY
                     RRRIGELKIDMPLIASNHRDAYPLAAAHDVDFLHLPVSPETKSQQEARLLDEVRARDI
                     DFVVLARYMQILSAEFCAALTGRIINIHHSFLPGFKGARPYHQAHERGVKLIGATAHY
                     VTADLDDGPIIEQDVERVDHRMSADQLA"
     gene            complement(1032..2444)
                     /locus_tag="AC16PP_03460"
     CDS             complement(1032..2444)
                     /locus_tag="AC16PP_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6480711.1"
                     /translation="MAEPAKPERLKQSTLMLFGLPEYAVYLGSIPVGLYLPFVYSKDL
                     GLELVDVGLILMLARISDVITDPLIGYLSDRTGGRFGRRKPWLAAGALLMMVSAFQLF
                     NPNALSAAPVDNWHLLSWSVLLWLGWTMINIPYYAWGAELSDDYNERTRITGWRQAFG
                     FLGNVSVLAVPVIAGEITGYGSMPREGLTIIGSMALVALPTLIAVTLWRVPEREAYAK
                     PKASVLTHARAMSRNGSFILLFLGFMLMSLGTGWGSATFMLFATYVVGAEGQTQAILL
                     GYFGANVIALPLWVALAQRIGKKTTWLIGGVLFVIVTPCFLLLEHGDLWWFFLCLALY
                     GVAGGNFAALSMSMKADVIEIAARRSGENMAGSYIAVWSLGQKMVAALALGLALPFLQ
                     LLGFDPAGGNDAEQIRALALVYVVPPWLFYALAVVVIWRYPITASRLQRIRAAFERRD
                     ARLERKRIGRAGFAEQAEPT"
     gene            2596..3888
                     /locus_tag="AC16PP_03465"
     CDS             2596..3888
                     /locus_tag="AC16PP_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6480712.1"
                     /translation="MSQGQKTPYEPVVPEKTDDIDLLDVGLQNCPYHAYRVLRDDAPV
                     WQDPRTGFFVITRFEDLRQVLLDTEHFSNGANSNARDKLDSDRAMRMRELYETKGWVP
                     APTLAGRDDPNHKQMRSMFDEAFRPARINSMEPLVEGTARKLIDAFVDDGHCDWVKQF
                     AVPMPLIIIGVQMGAKEEDIWRIKAWTDAWVQRLGMMQTEEEERWSVEMEIEAQNYFQ
                     PIFERLRKEPDDSLISDLVNRVIPEWGRPLNDNELHAEMMADTFVGGSETSTNALSYG
                     VKLLIENPDSWDRLKSDPDKYLRTFCEEVLRLEGPVQGLFRRAAKDIELHGVQIPEGS
                     MINIRYAAANRDEREFENPEKLDLDRKKPGRHLAFGSGIHHCLGAPLARRELYWGFKV
                     LVERIDEMWFAPGKNDFTVAPNFSLRALKELHIEFNKA"
     gene            3899..4669
                     /locus_tag="AC16PP_03470"
     CDS             3899..4669
                     /locus_tag="AC16PP_03470"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6480713.1"
                     /translation="MNMHDKFRLDGQVAVVTGGGRGIGRGIALALADCGADVVVVARR
                     QADVEEVAEEIRERGRRGLGVSADVMDFTAIPGVLDRVVEEFGRLDIMVNNAGGNLDR
                     KTYSLPDIPIEKFDEQLTLNMKTKWWGAQQAAKRMENGGRIINIISVAAHRAAPGFGV
                     YSAANMGMISMTRTLAVELAPKKITVNCIAPGIIVTEMLKETMHISEEEAHQTFDAQI
                     PLGRTGTPEDCAAAAVFFASPAAEWTTGQFLDVAGGPA"
     gene            complement(4756..>5373)
                     /locus_tag="AC16PP_03475"
     CDS             complement(4756..>5373)
                     /locus_tag="AC16PP_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6480714.1"
                     /translation="AQRRAKILQATRDLVTEHGYDGMIMRDVAALADVSATTLYNLYN
                     TKDELLLEALRDRIEASTVQARQHAPEPGYEYLLAHVRSVCRQTRRSPAYVNAIAQAL
                     FRSTPGDPLAEVLLAGLQEDVERSLQAMSDAGHLHPQARINALARALTGSFWSNFLLW
                     NKGRLALKDLETAQLSGYLSLLIPTTVGSANEALRSRLKALSGLS"
BASE COUNT         1069 a         1585 c         1802 g          917 t
ORIGIN      
        1 tgcgagctgg tcggccgaca tgcggtggtc gacgcgctcc acgtcctgtt cgatgatggg
       61 cccgtcgtcc aggtccgctg tgacgtaatg cgcggtagcg ccgatcagct tgacgccccg
      121 ctcgtgagcc tggtgataag gacgggcgcc cttgaagccg ggcagaaacg agtgatggat
      181 gttgatgatc cggccggtca gggcggcgca gaactcggcg ctgagtatct gcatgtagcg
      241 ggccaggacg acgaagtcga tgtcccgggc tcgcacttcg tccaggagac gcgcctcctg
      301 ctgcgatttg gtctcggggc tgaccggaag gtgcagaaag tcgacgtcat gggcggccgc
      361 cagcgggtag gcgtcgcgat gattcgaggc gatcaagggc atatcgatct tcagctcccc
      421 gattcgcctg cggtacagga gatcggccag gcagtggtct atcttggaga ccagcatgag
      481 cactcggggc cgtcgcgcgg ttggccagat ctgccaatcc atgttcatgg caccggcagt
      541 cgggatgaac gtctgattca aggcgggaag gtcgtcgggc gcctcgaaat gcacccgcat
      601 gaaaaagcgt tccgtggtgt cgtcacggaa ctgatcactg tcgagtatgt tcccgttcag
      661 gtccaccagc cagctcgata ccgcatgaac cagcccggga cgatcgggac atgagaaagt
      721 gagaacgtaa tcagccaagc acgaacacgc cgcaattcca gcggcgtgca tcgtacctgc
      781 gatcagcctc caaagcgaac gcgaatggtc cttccggcaa tggcaagcgc catgccgccc
      841 gcccttacgg cgatgttcag accttgatca gccccgctgc gatggcgatg agcgtgatcg
      901 tgatcccggt aacggccacg aggccaagtg ccatgctgat gtgttgcgcg gtttcgacca
      961 tgattgatct ccagccattg attgatcatg acctttttaa ggctgatgac ggctttggag
     1021 aatcagggga tctacgtagg ctccgcctgc tcggcgaaac cagctcggcc gatgcgctta
     1081 cgctccaggc gcgcgtcgcg gcgttcgaaa gccgcccgga ttcgttgcag gcgggaggcc
     1141 gtgatcgggt agcgccatat caccaccacc gccaacgcgt aaaacagcca gggaggaacc
     1201 acatagacga gcgcgagggc cctgatctgc tcggcatcgt tgccgccggc agggtcgaaa
     1261 ccgagcagtt gcagaaaggg tagcgccaga cccagagcca gggcggcgac catcttctgg
     1321 ccgagggacc agaccgcgat gtaggaaccc gccatgttct cgccggaccg gcgcgcggcg
     1381 atctcgatca cgtctgcctt catggacatc gacagcgcgg cgaaattgcc cccggccacc
     1441 ccgtagagtg ccaggcaaag gaagaaccac cagagatcgc cgtgctccag cagcaggaag
     1501 cagggggtga cgatgacgaa gagcacccct ccgatcagcc aggtggtttt cttgccgatt
     1561 cgctgggcga gggccaccca cagaggcagg gcgataacgt tcgcgccgaa gtaaccgagc
     1621 agaatggcct gagtctgtcc ctcggccccg acgacgtagg tggcgaacaa catgaacgtc
     1681 gccgatcccc agccggtacc gagggacatg agcatgaatc ccaggaacag aaggatgaag
     1741 gagccgttgc gtgacatcgc ccgcgcgtgg gtgagcaccg atgccttcgg cttcgcgtag
     1801 gcctcgcgtt cggggacgcg ccagagggtc acggcgatca acgtgggcag cgccacgagc
     1861 gccatggatc cgatgatcgt caggccctct ctgggcatgc tgccgtatcc agtgatctcg
     1921 ccggcaatga cgggcactgc caggacgctc acgttcccga ggaagccgaa cgcctgccgc
     1981 catccggtga tgcgggtgcg ttcgttgtag tcgtcggaca gctcggcgcc ccacgcgtag
     2041 taggggatgt tgatcatggt ccagccaagc cagagcagca cgctccagct caacaggtgc
     2101 cagttgtcca ccggtgctgc gctgagcgcg ttcgggttga agagctggaa tgccgagacc
     2161 atcatcagga gcgcgcccgc cgcgagccag ggcttgcggc ggccgaagcg gcctcctgtg
     2221 cggtcgctga gatagccgat caacgggtcg gtgatcacat cggagatgcg cgccagcatc
     2281 agaatgaggc ccacgtcgac cagctccagg ccgaggtctt tggagtagac gaaaggcaga
     2341 tacagcccga ccgggattga tccgaggtag acggcgtatt ccgggagccc gaacaacatc
     2401 agcgtggact gcttcaggcg ctctggtttt gcgggctcgg ccaccggtgg ctccacgaag
     2461 ctgacgaacc aaggctaggg ggtgatgctt cgtggggtca acaaaatttc tggtgagcgc
     2521 tacaataaat tgccgttctg gatggccagg acggacaatg gccccatggg gccgcgcatc
     2581 acaggggagg acgccgtgtc acaagggcag aagacaccat acgagccggt agttccagag
     2641 aagaccgacg acatcgacct gctggatgtc ggtctgcaga attgtccgta ccacgcctat
     2701 cgggtcttga gagacgacgc ccccgtgtgg caggacccga ggacgggctt tttcgtgatt
     2761 acccgattcg aggatctgcg acaggttctg ctggataccg agcacttctc gaacggggca
     2821 aacagcaacg cgcgcgacaa gctcgattcc gatcgggcca tgcggatgcg cgagctctac
     2881 gaaaccaagg gatgggttcc ggcgccgacc ctggccggtc gcgacgatcc gaaccacaag
     2941 cagatgcggt ccatgttcga cgaagcgttc aggccggcgc ggataaactc catggaaccg
     3001 cttgtagagg gcacggcgcg caagctcatc gacgcctttg tcgacgatgg acattgcgac
     3061 tgggtcaagc agttcgccgt ccccatgccg ctgatcatca tcggcgtgca gatgggggcc
     3121 aaggaggagg acatctggcg gatcaaggcg tggacggatg cctgggtcca gcgcctcgga
     3181 atgatgcaga ccgaggaaga ggagcgctgg tccgtggaaa tggagatcga ggcgcagaac
     3241 tactttcagc cgatcttcga gcgacttcgc aaggagccgg acgattctct catctcggat
     3301 ctcgtgaacc gtgtcattcc cgagtggggc aggccgctca acgacaatga gctgcacgcc
     3361 gagatgatgg ccgatacctt cgtcggaggc tcggagacct ccacgaacgc gctgtcctac
     3421 ggagtgaagc tgctcatcga gaaccccgat tcctgggacc ggctgaagtc ggatccggat
     3481 aaataccttc gtacgttctg cgaggaggtg ctccggctcg aaggcccggt gcaggggttg
     3541 ttccggagag cagccaaaga catagagctg cacggcgtgc agataccgga aggatcaatg
     3601 atcaacatca gatacgcggc tgcgaaccgg gacgagcggg aattcgagaa tccggagaag
     3661 ctcgatctcg atcgcaagaa gcccgggcgc cacctcgcgt tcggttccgg catacaccac
     3721 tgcctcgggg ctccgctggc gcggcgcgag ctctactggg gtttcaaggt gctcgtggag
     3781 cggatagacg agatgtggtt tgcccccggt aagaacgact tcaccgttgc accgaatttc
     3841 tctctgcgcg ctctcaagga gctgcacatc gagttcaaca aggcctagga ggcgcgaaat
     3901 gaacatgcac gacaagttcc gactggatgg tcaggtagcc gtggtcaccg gtggtggacg
     3961 cgggatcgga cggggaatcg cgctggcact ggccgactgc ggagcggatg tggtggtggt
     4021 cgcccgccgt caggcagacg tcgaagaggt ggcggaggag attcgcgagc ggggccggcg
     4081 cgggctgggc gtctccgcgg acgtcatgga cttcacggcc attcccgggg tcctggaccg
     4141 ggtggtggaa gagttcggcc gtctggacat catggtgaac aacgcggggg ggaatctgga
     4201 tcggaaaacg tactccctgc ccgacatacc catagagaag ttcgacgagc agctcaccct
     4261 gaacatgaag accaagtggt ggggtgctca gcaggcggcc aagcgaatgg agaacggtgg
     4321 ccgcatcatc aacatcattt ccgtggccgc ccatcgagcg gcgccggggt tcggcgtcta
     4381 ctctgcggcc aatatgggga tgatcagcat gacccggacg ctggcggtgg agcttgcgcc
     4441 caagaaaatc accgtgaact gcatcgcgcc cggcatcatc gtcaccgaga tgctcaagga
     4501 aaccatgcac atttccgagg aggaagccca ccagactttc gatgcgcaga ttccgctcgg
     4561 gcggacgggc actccggagg actgcgcagc cgctgccgtg ttctttgcgt ccccggcggc
     4621 ggagtggacc accggccagt tcctagacgt cgcggggggg cccgcctagg gccgacctgg
     4681 gctagccgac cgcgtctggc agaccgcgtc tagccgaccg cgtctagccg accgcgtcta
     4741 gccgaccgct tgggttcagc tcagaccgct cagagccttc aggcgactgc gcaaggcttc
     4801 gttggcggag ccgacggtgg tcgggatcag cagggaaagg tagccgctca gctgggcggt
     4861 ttccaggtcc ttgagcgcga gccggccctt gttccacagc agaaagttgg accagaagga
     4921 acccgtcagg gctcgggcga gcgcgttgat gcgcgcttgg gggtgcaggt gcccggcgtc
     4981 gctcatcgct tgtagcgacc gctccacgtc ctcctgcagc cccgccagca gcacctcggc
     5041 aagggggtct cccggagtgg agcgaaacag cgcctgggcg atcgcgttca cgtaggcagg
     5101 ggagcggcgg gtctgccggc agacgctgcg gacgtgggcc aggaggtact cgtagcccgg
     5161 ttcgggagcg tgctgacggg cctgaacggt gcttgcttcg atcctgtcgc gcagggcctc
     5221 caaaagcagc tcgtccttgg tgttgtagag gttgtaaagg gtcgtagccg agacgtctgc
     5281 gagggcggcc acgtcccgca tgatcatgcc atcgtagccg tgctcggtta ccagatcccg
     5341 ggtagcctgc aggatcttcg cccgccgctg ggc
//
LOCUS       DBNHKV010000108         4398 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_582215, whole genome shotgun sequence.
ACCESSION   DBNHKV010000108 DBNHKV010000000
VERSION     DBNHKV010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_582215"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="AC16PP_03480"
     CDS             complement(<1..707)
                     /locus_tag="AC16PP_03480"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR6480715.1"
                     /translation="MTRFIFVTGGVVSSLGKGIASSSLAALLEARQLNVNVLKMDPYI
                     NVDPGTMSPFQHGEVFVTADGAETDLDLGNYERFTHARMSRRNNFTTGSVYAEVLRRE
                     RRGDYLGGTVQVIPHITDEIKRRINEAGQGFDVLIVECGGTVGDIESQPFLEAIRQMR
                     LEVGAANAIIIHLVLVPFVPSAGEIKTKPTQHSVKELRSIGIQPDILLCRADRPIPST
                     ERRKIALFTNVPERAVIS"
     gene            complement(789..2066)
                     /gene="tilS"
                     /locus_tag="AC16PP_03485"
     CDS             complement(789..2066)
                     /gene="tilS"
                     /locus_tag="AC16PP_03485"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR6480716.1"
                     /translation="MDAGQLVEEALRRALRGAVAPGRVFVGYSGGMDSTVLLHAAIAA
                     SPVPVTAVHANHGLDPQAGRWQAHCERVCAQWGVGLESREVSVGHGNVEAAARAARYG
                     FFESVLSASDLLLLAHHQDDQAETVMLRMVQGRGLIGIPSARRLGAGELRRPFLELPR
                     QVLAEYADGLALDWVEDPGNADEGLDRNYLRSRVLPSLRRRWPGFAGQMQVLLDQRAS
                     TEQMLLARVDGDFESIAVGELLCGDGGSGAELLGIWLSSLGAALPSRGALASFVAQLG
                     SAADRQPELKLAQGSLRRYRGRVYRVADPPALAPSYELIPPGELRLPHGTVTVCEAHG
                     GGFQLHGPLKVMFRGPEGAGHIRCRGHRRSIKKLLQDAGLPPWKRLTYPLLEDADGVA
                     AVPGIAERDPDPDPDPPSGGTRWVARWTAHSIR"
     gene            complement(2071..>4398)
                     /gene="dnaE"
                     /locus_tag="AC16PP_03490"
     CDS             complement(2071..>4398)
                     /gene="dnaE"
                     /locus_tag="AC16PP_03490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR6480717.1"
                     /translation="FLNPERVSMPDFDIDFCMEGRDRVISHVSERYGHDAVSQIITFG
                     TMAAKAVVRDVARVQGKPYGLADKLSKLIPFEVGMTLEKAVSQSSELADFIASSEDVG
                     EIMDMAYKLEGIVRGLGRHAGGVVIAPSALTDFVPLYMDDQSGGLVSQFDKDDVESAG
                     LVKFDFLGLKTLTIIDWAVQAVNAARGDADELLDIDHIPLDDPGTFEFLRTAETTGVF
                     QLESSGMKDLIRRLKPDSIDDIIALVALFRPGPLQSGAVDDYIDRKHGQEPITYPHPE
                     MKAVLQNTYGVMLYQEQVMQTAQVLAGFSLGQADLLRRAMGKKKPEEMAKVREQFLSG
                     CSERGVDAALAGQIFDLMEKFSGYAFNKSHSATYALVSFQTAWLKTHHPAEFMAATLS
                     VDMQNTDKVVTLVDETRNMGLPLSPPSVNRSEYRFSAREGAVVYGLGAVRGVGEGPVQ
                     ALVEARASGGAFESLPDFCRRVDSRKANRRVLEALIRAGAMDDFAEPGEALDCLRARL
                     MAELGDALHGAEQAARDVALGMSDMFGGLEEPTLGNGNGRGAPPALGKRERLEWEKEA
                     LGLYLSGHPIEDYLGEIREFCGRPIADLEAGKRTQVVAGLVFSARTMRGRRGEMGFVV
                     LDDRSKRIEVSLFSEVFQAGKEKVAKDAVLVVEGEVQPDDYTGELKMRADNVYTIEEA
                     RRRFARELVIDCCDASPTADFSSRLKTCLEPYRDGSQGCSIAVMYQSPATSGPGARGR
                     IVLGRDWAVIPCDDLLASLRSEFGDDRVAFRYAGN"
BASE COUNT          774 a         1503 c         1384 g          737 t
ORIGIN      
        1 gagatcacgg cccgctcggg gacattggtg aacaatgcga tcttgcggcg ctcggtgctt
       61 ggaatgggcc ggtcggcgcg gcacagcagg atatcgggct ggatgcctat ggagcgcagc
      121 tccttcaccg agtgctgggt gggcttggtc ttgatctccc cggcggacgg cacgaagggg
      181 acgagcacca gatgaatgat gatggcgttc gccgcgccca cttccagccg catctggcga
      241 atggcctcca ggaacggctg cgactcgatg tcaccaaccg tccccccaca ctctacgatc
      301 agcacatcga agccctgccc ggcttcgttg atcctgcgtt tgatctcgtc ggtgatgtgc
      361 gggatcacct gaaccgttcc gcccaggtag tctccacggc gctcccgccg cagcacctcg
      421 gcgtagacgc tgccagtggt gaagttgttc cgccgcgaca tgcgggcgtg ggtgaaacgc
      481 tcgtagttgc cgagatccag atccgtttcc gccccgtcgg cggtcacgaa gacttcaccg
      541 tgctgaaacg ggctcatcgt cccgggatcc acattgatgt agggatccat cttcaacacg
      601 tttacgttga gttgccttgc ctcgaggagc gctgcgaggg acgacgaggc gataccttta
      661 cccagagagg atacgacacc gccagtaacg aaaatgaatc gtgtcatcta ttccatcttg
      721 aagcccgagg tccgcgattg ctgcgaacgc aagatggcct tgaaagataa caaaagcccc
      781 cggcaacctc aacgaatcga atgcgccgtc catcgggcga cccagcgcgt ccccccggac
      841 ggcgggtccg ggtccgggtc cgggtcccgc tccgcaatgc ccggaaccgc ggctaccccg
      901 tctgcgtcct cgagcagcgg ataggtgagc cttttccagg gcggaagacc cgcatcctgg
      961 agcagtttct tgatggagcg gcgatggccg cggcagcgga tgtgtcctgc tccttcaggg
     1021 ccgcggaaca tcaccttgag cgggccgtgt aactgaaagc ccccgccgtg cgcctcgcac
     1081 acggtgacgg tgccgtgggg cagccgcagc tcccccggcg ggatcagctc gtagctcggc
     1141 gccagagcgg gcggatccgc gacccgatag acgcggcccc ggtagcggcg cagagacccc
     1201 tgcgccagct tgagctccgg ttgccggtcg gcggcgcttc cgagctgcgc cacgaacgag
     1261 gcgagggcgc ctcgggaggg gagcgcggcc ccgagagagc tcagccagat gcccaacagc
     1321 tccgccccgg agccgccgtc cccgcaaagg agctcgccga cggcaatgga ctcgaagtcg
     1381 ccgtccaccc ttgccagcag catctgctcg gtgctcgccc gctgatccag cagaacctgc
     1441 atctgcccgg caaaccccgg ccagcgccgg cgcaggctcg gcaggacgcg actgcgcaga
     1501 tagttccggt ccagaccttc gtcggcattg ccgggatcct ctacccaatc gagggcgagc
     1561 ccgtcggcat actccgccag cacctgccgc ggcagttcca gaaatggtcg ccgcagctcg
     1621 ccagcgccca gccgacgggc agacgggata ccaatcagcc cccggccctg aaccattcgc
     1681 agcatcacgg tctccgcctg gtcgtcctgg tgatgagcca gcagcagcaa gtcggatgcg
     1741 gagagaaccg attcgaagaa gccgtaacgg gcggcccggg cagcggcttc cacgttgccg
     1801 tggcctaccg acacttcgcg ggattcaaga cccacccccc actgcgcgca gacccgctcg
     1861 caatgtgcct gccatcgccc ggcctgggga tccaggccgt gattggcatg cacagcggtg
     1921 acgggcactg gagacgcggc gatcgccgcg tgcagcagaa ccgtggagtc catgccgccg
     1981 ctgtagccga cgaagactcg gcccggtgcg acggcaccgc gcaatgccct tcgcagcgcc
     2041 tcttcgacca gctgcccggc gtccacggga tcagttcccg gcgtatctga aagccacgcg
     2101 atcgtcgcca aattccgagc ggaggctcgc aagaagatca tcgcagggga tgacggccca
     2161 gtcgcgacca agcacgatgc ggccccgggc cccgggcccg gaggtcgccg gcgactggta
     2221 catgaccgct atggagcatc cctgggaacc atcgcgataa ggctccaggc aggtcttcag
     2281 acgggaggag aaatcggctg tcggggacgc gtcgcagcag tcgatgacga gctcgcgggc
     2341 gaagcgccgc cgtgcctcct cgatcgtgta gacgttgtcg gcccgcattt tgagctctcc
     2401 ggtgtagtcg tccggctgaa cctccccctc cacgacgagc acggcgtcct tggcgacctt
     2461 ctccttgccc gcctggaaga cctccgagaa cagcgagact tcgatccgct tgctgcgatc
     2521 gtccagaacc acgaaaccca tctcgccccg gcggccgcgc atcgtgcgcg cggaaaaaac
     2581 cagtccggcg acgacctggg tgcgcttgcc ggcctcgagg tccgcgatgg ggcggccgca
     2641 gaactccctg atctccccaa ggtagtcttc gatggggtgg ccgctgaggt agaggccgag
     2701 cgcttccttc tcccactcca ggcgctcccg cttgccgagc gctggcggcg ccccacggcc
     2761 gttgccgttg ccgagggtgg gctcctccag cccgccgaac atgtcggaca tgcccagagc
     2821 gacatcccgc gcggcctgct cggccccgtg caacgcgtcg ccaagctcgg ccatgaggcg
     2881 cgcgcgcaag cagtctaagg cttctccggg ctcggcaaag tcgtccatgg cccccgcgcg
     2941 gatcagcgct tccagaaccc gccggttggc tttgcgagag tcgacgcggc ggcagaagtc
     3001 gggaaggctt tcgaacgcgc cgcccgacgc gcgcgcctcg acgagtgcct gcacggggcc
     3061 ctcgcccacg ccccgcacgg cgccaagacc gtaaactacg gcgccctccc ttgccgagaa
     3121 ccggtactcg ctgcgattga cgctcggcgg gctgagcggc agccccatgt ttctcgtctc
     3181 gtccaccaga gtcaccacct tgtcggtgtt ctgcatgtcc acagacaggg tggccgccat
     3241 gaactccgcc ggatggtggg tcttcagcca cgcggtctga aaggaaacga gggcgtaggt
     3301 cgcgctgtgc gacttgttga aggcgtaacc ggagaacttc tccataaggt cgaagatctg
     3361 gccggcgaga gcggcatcaa cgccacgctc gctgcagccg gagagaaact gctcgcgcac
     3421 cttggccatc tcttcaggct tcttcttccc catggcgcgc cgcaacaggt cggcctgacc
     3481 gaggctgaac ccggcgagca cctgggcggt ctgcatgacc tgctcctgat agagcatcac
     3541 gccgtaggtg ttctgcagca cggctttcat ctcggggtgc ggatacgtta tgggttcctg
     3601 accatgcttg cgatcgatgt agtcgtccac cgcaccggac tggagcggac cgggccggaa
     3661 cagggccacc agcgcgatga tgtcgtcgat gctgtccggc ttcagccgcc tgatcagatc
     3721 cttcatcccg gaggattcca gctgaaacac cccggtggtt tcggccgtac gcaggaactc
     3781 gaaggtcccc gggtcatcca gagggatgtg gtcgatgtcc aacagctcgt ctgcgtctcc
     3841 ccgcgccgcg ttcactgcct gcacggccca gtcgatgatg gtcagcgtct tcaggccgag
     3901 gaagtcgaac ttgaccaggc ccgcgctctc cacgtcgtcc ttgtcgaact gcgaaactaa
     3961 cccgccgctc tgatcgtcca tgtacagggg gacgaagtcc gtcagcgccg acggcgcgat
     4021 gaccacgcct ccggcgtggc gccccaggcc ccgaacgatt ccttccagct tgtaagccat
     4081 gtccatgatc tcacccacgt cctcgctgga agcgatgaaa tccgcaagct cgctgctctg
     4141 cgacactgcc ttttccagcg tcatgccgac ttcgaacggg atcagcttgg agagcttgtc
     4201 ggccagaccg tagggcttgc cctgaacccg ggcgacgtcc cgaaccaccg ctttagcggc
     4261 catggttccg aacgtgatga tctggctgac ggcatcgtgg ccgtagcgct cggacacgtg
     4321 gctgatgacc cggtcacggc cctccatgca gaagtcgatg tcgaagtccg gcatggaaac
     4381 ccgttccggg ttgaggaa
//
LOCUS       DBNHKV010000109        10115 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1220667, whole genome shotgun sequence.
ACCESSION   DBNHKV010000109 DBNHKV010000000
VERSION     DBNHKV010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10115)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10115)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10115
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1220667"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="AC16PP_03495"
     CDS             <1..702
                     /locus_tag="AC16PP_03495"
                     /inference="COORDINATES: protein motif:HMM:NF019032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HIR6480718.1"
                     /translation="LIGAVIAAVLLVAGALGTRIGLWDFQVGFLGLAGGTILAAIGVI
                     VGAVGLIASVKRELPQNRPPIYLGLAISVVILIVMGMQFDKAYSVPPIHNISTDLEDP
                     PAFDQVVALRGEESNPLALNAQEIGPMQREAYPWVVPLVTPTARDEMFSRALAVVDDM
                     GFELVNADPAAGIIEATDTTFWFGFKDDVAIRVRSAEAGTVVDVRSVSRVGLSDLGAN
                     AKRIGDILAALGAGA"
     gene            complement(710..1195)
                     /locus_tag="AC16PP_03500"
     CDS             complement(710..1195)
                     /locus_tag="AC16PP_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HIR6480719.1"
                     /translation="MKIGVVSDTHNNLRNVGRIVELFNEYGVERVIHTGDITQAKTLD
                     VFARLTAPLWGVYGNNDQERDSLEQAVAAHGFSFTEPPLHLTWHGREIVVVHDPLEFD
                     GVLNDGHELALHGHTHLYRYEERASQVIFNPGECAGHMAGFNAVGVVDLSDLSAELLR
                     F"
     gene            complement(1286..2431)
                     /locus_tag="AC16PP_03505"
     CDS             complement(1286..2431)
                     /locus_tag="AC16PP_03505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012336.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6480720.1"
                     /translation="MTDAYVVGVDMIKFGRFPEKTVPEIGAESALMALDDCGLTIQDM
                     EALYCGNLGQASGMVGQRILQQIGQTGIPVVNVSNACATGATAFREAWASVKAGLYDC
                     VLAVGVEQMGKGLLGGAGGGTGIPKEGLLGSGTMPAVFAEAGMEHTRKYGTTFEQFAK
                     VSVKNHHHSTMNPKALYQIETPLETVMNAEMISYPNTKLMCSVNVDGSAAAVICSEAK
                     AKELGLMKRAVKVRASVLTSDPWQERDLVMPDVNSCTRKAAAAAYEMAGLGPDDIDLI
                     ELHDCFATAEILHYENLGLCGDGEAGRLIDDGEVALGGRIPVNVSGGLLSKGHPLGAT
                     GIANIYEVCTHLRGEAGQRQVEGARIGLTHVIGLGSACGIHILEKTA"
     gene            complement(2456..2872)
                     /locus_tag="AC16PP_03510"
     CDS             complement(2456..2872)
                     /locus_tag="AC16PP_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6480721.1"
                     /translation="MSDTPLPVVEYLKIPEDGDPYLEGYKCGSCGATFLGERSVCSKC
                     GARDQMSAVTLPNHGTLYSYSIVHRSFPGIDVPYVSAVVDLDDGTAIKGNLINVEPDP
                     DKIEFGMPVDVVFADALGRKDRDGNSYLSFFFQPSS"
     gene            3015..3455
                     /locus_tag="AC16PP_03515"
     CDS             3015..3455
                     /locus_tag="AC16PP_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6480722.1"
                     /translation="MKSVKVADYMSTRLITFNRTDSVVEAMAVLLKKKISGAPVVDDE
                     GNLIGILSETDLMAVVIQDSYYDEGVGIVGDYMREEVDTVDPDSDIYSLADRFHKEGR
                     RRFPVVKNGRLVGQISRRDVLRAAEEFLMHADGLSERKRRKLLT"
     gene            complement(3452..4912)
                     /gene="gatB"
                     /locus_tag="AC16PP_03520"
     CDS             complement(3452..4912)
                     /gene="gatB"
                     /locus_tag="AC16PP_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR6480723.1"
                     /translation="MGFRIPQGWEAVIGLEVHVQLATRSKIFSGASTRFGAEPNTQAC
                     PVDLGLPGVLPVLNAEAVRMAVTFGLAVGAEIRPYSVFDRKNYFYPDLPKGYQISQLP
                     HPIVGRGAITIEASGEAPRTIGITRAHLEEDAGKSIHDAYAGLTGIDLNRAGTPLLEV
                     VSEPELRTPEEAGAYFRKLHSLVRHLKICDGNLAEGSMRCDANVSVRPAGETALGQRT
                     EIKNVNSFRFLERAIEFEIERQIDVLEAGGAIEQQTLLYDPDSHETRPMRTKEYSEDY
                     RYFPDPDLLPVHVSEAFIESVRSTMPELPDARRARYAAEFQLGDYDAAWLTQDPEVAD
                     YFEAAAASAGDAKLAANWVMGDLSAALNREELGIARSPISPSQLGDLIGRIRDETISG
                     KIAKTVFEGLWAGEGEVDEIIAARGLTQVSDAGEIEAIIAGIVAENPDQVAQFRAGKE
                     KVLGFFVGQVMKATQGKANPKQVNELLRKALAADTG"
     gene            complement(4914..6371)
                     /gene="gatA"
                     /locus_tag="AC16PP_03525"
     CDS             complement(4914..6371)
                     /gene="gatA"
                     /locus_tag="AC16PP_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR6480724.1"
                     /translation="MDPFASIGQHAARLRDGSYSSVELTRFFVDRAREHADLNAFICV
                     DEEGALAAAEAADARLRAGTATALTGIPLAHKDIFCTRGTITSCASKMLENFVAPYDA
                     TVVERLNAAGAVTVGKANMDEFAMGSSNENSRFGPVRNPWDPGRVPGGSSGGSAAAVA
                     AGLVPVASGTDTGGSIRQPSAFCGVSGIKPTYGRVPRYGMVAFASSLDQGGFLGRSAE
                     DLALSLAFCAGADPRDSTCSEHVDEWLDGVAENGVGNLEGGLRIGLPREYFADLSTGS
                     ELLDAVAGFFESIGCSLEEVSLPHTSAAIPAYYIIAGAEASTNLSRYDGVRFGYRCEN
                     PGSLEDLYRRSRSEGFGAEVKRRILTGTYALSVGYYDAYYVKAQRLRRLIQRDFLEAF
                     ETVDMILAPTAPGVAFPLGELTHDPVAMYQQDIFTIPASLAGLPAMSLPCGFHQGLPI
                     GVQLIGPHFAEKTLLQAGAAYQRETDWHTRHPEAG"
     gene            complement(6373..6654)
                     /gene="gatC"
                     /locus_tag="AC16PP_03530"
     CDS             complement(6373..6654)
                     /gene="gatC"
                     /locus_tag="AC16PP_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003120927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR6480725.1"
                     /translation="MASIEIAHLCRLAQLALTESEQAAALADLQRIIAMIDEMQAMDT
                     DGVEPLAHPIESDQRLRPDTVSETVDRDHYQRGAPATRDGLYLVPRVVE"
     gene            6824..7864
                     /locus_tag="AC16PP_03535"
     CDS             6824..7864
                     /locus_tag="AC16PP_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR6480726.1"
                     /translation="MFKNIRGLFSRDLSIDLGTANTLIYVRDQGIVLNEPSVVAIRVQ
                     GNQKSVAAVGLDAKRMLGRTPGNITAIRPLKDGVIADFLVTEKMLKYFIAKVHENSFI
                     RPSPRVLVCVPCKSTEVERRAIRESALSAGARDVRLLEEPMAAAIGAGLPVQEAVGTM
                     VVDIGGGTTEIAIISLNGVVYSDTVRVGGDRFDESIVAYVRRTQGSLIGEATAERIKQ
                     EIGAAYPQKEVREIDVRGRNLAEGVPRSFTLNSNEILEALQEPLSMIVQALKSALEQC
                     PPELASDIAETGLVLTGGGALLRDLDTLLAEETGLPVIVAEDPLTCVARGGGKALELM
                     DKAGNADLLSTE"
     gene            7917..8720
                     /gene="mreC"
                     /locus_tag="AC16PP_03540"
     CDS             7917..8720
                     /gene="mreC"
                     /locus_tag="AC16PP_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR6480727.1"
                     /translation="MLTGLVLVSLILVTLDLTTSFMRGTRQVFGTMLAPLHYVAETPY
                     LVGEELGNVFATRSALLDENQMLKRQVLELSKISLQYRNLLKENENLRELLGSRARLP
                     DEVLIAEIIGIVPTVGAHQVVIDKGSDAGVQIGQAVIDSEGLFGQVVEVDVYSSRVLL
                     ITDRDHAVPVQINRNGIRSIAGGTGRMDQLELENVTITADVVKGDLVETSGLGGRFPE
                     GYPVGEVESVVVDPAASLSRVLVRPSALLDRSRHVLVVFAPETRDEVLP"
     gene            8717..9211
                     /gene="mreD"
                     /locus_tag="AC16PP_03545"
     CDS             8717..9211
                     /gene="mreD"
                     /locus_tag="AC16PP_03545"
                     /inference="COORDINATES: protein motif:HMM:NF016019.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR6480728.1"
                     /translation="MNPAQGGWLILASLVIALVLSIFHLPETWPQWLGWLRPAWVPLF
                     VFFWVMALPHRLGLVSAWIFGIFVDVLFGDPIGLNGAVLAGITYIGWRFYERLRMYSV
                     VQQCGVVFLLILGGDLVRMLVHDLSADRGWSWAVLVPALTSMLVWPFVSLFLQRLRLQ
                     FGVE"
     gene            9286..9858
                     /locus_tag="AC16PP_03550"
     CDS             9286..9858
                     /locus_tag="AC16PP_03550"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007998187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR6480729.1"
                     /translation="MVLASGSPRRAELLRQIGLPFVVRPPDVDESLRPGESPERYVER
                     LAIGKARAVVAEDGPVLGADTAVVVDEEILGKPADRASAIDMILRLSGRTHRVLTGVA
                     VTDGRAVRSCVVAADVTFRAITRDEAAAYWETGEPRDKAGGYGIQGIGGIFAQSINGS
                     YSAVVGLPLAETEALLHAIGVNTWELRASV"
     gene            9851..>10115
                     /locus_tag="AC16PP_03555"
     CDS             9851..>10115
                     /locus_tag="AC16PP_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="involved in the processing of the 5'end of 16S
                     rRNA; Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR6480730.1"
                     /translation="MSEELLINVNDFESRVALVSGSVVEEVQVARAGADSLTGNIYLG
                     RVQRIIPGMQAAFVDCGLERPGFLHVSDIAGPRLMLGEEPDGAV"
BASE COUNT         1620 a         3187 c         3502 g         1806 t
ORIGIN      
        1 ctcatcggcg cggtgatcgc cgccgtcctg ctggtggcgg gcgcgctggg cacccgcatc
       61 gggctgtggg attttcaggt gggcttcctg ggtctcgcgg gcggaacgat cctggccgcc
      121 atcggcgtca tcgtcggtgc cgtgggcctc atcgcctcgg tgaagcggga gcttccccag
      181 aaccgaccgc ccatctactt gggcctcgcg atcagcgttg tgatactgat cgtcatgggc
      241 atgcagttcg acaaggccta ctccgtgccg ccgatccaca acatctccac ggatctggaa
      301 gacccgccgg ccttcgacca ggtggtcgcg ctgcgcggcg aggagtccaa cccgctggcg
      361 ctgaacgccc aggagatcgg gcccatgcag cgtgaagcct atccgtgggt cgtgcccctg
      421 gtgaccccga cagcgcggga cgagatgttt tcccgggctc tggccgtggt ggacgacatg
      481 ggcttcgagc tcgtgaacgc cgatcctgcc gcgggcatca tcgaggcgac ggacaccacc
      541 ttctggttcg gcttcaagga cgacgtcgcc atccgcgtgc gatcggccga ggcgggcacc
      601 gtggtggacg ttcgcagcgt atcccgggtg ggattgagcg atctgggcgc aaacgccaag
      661 cggatcggag acatcctcgc agccctgggg gccggggcgt aaaccggggc tagaagcgca
      721 gcagctcggc gctcagatcc gacaggtcaa ccacgcccac ggcgttgaac ccggccatgt
      781 ggcccgcgca ctcgccggga ttgaagatga cctggctggc gcgctcctcg tagcggtaca
      841 ggtgggtgtg gccgtgcagc gccagctcgt gcccgtcgtt cagaacgccg tcgaactcca
      901 gggggtcgtg caccaccacg atctcccggc cgtgccaggt gagatgtagc ggcggctccg
      961 tgaatgagaa gccgtgggcc gccacggcct gctcgaggga gtcccgttcc tgatcgttgt
     1021 ttccgtagac gccccacagg ggcgcggtga gccgcgcgaa gacgtccagg gtcttggcct
     1081 gggtgatgtc gccggtgtgg atcacgcgct cgacgccgta ctcgttgaag agctccacga
     1141 ttcgacccac gttgcgcagg ttgttgtggg tgtcgctgac gacgccgatt ttcatgggct
     1201 tggtggttgg aggcgaaggg ccggccttca gggcggcccc ggcccgacgg ccggggcggc
     1261 cacttgggac ggcgatggct gtggtttagg ccgtcttctc gaggatgtga atgccgcagg
     1321 cgctgcccag cccgatcacg tgggtaagcc cgatgcgcgc gccctcgacc tgccgttgtc
     1381 cggcctcgcc acgcaggtgg gtgcagacct cgtagatgtt ggcgatcccg gtcgcgccca
     1441 gggggtggcc cttggagagc agcccgccgg agacgttcac cgggatgcgg ccgccgagcg
     1501 ccacctcgcc gtcgtcgatc agccggcccg cttcaccgtc accgcaaaga cccaaattct
     1561 cgtagtggag gatctcggcg gtggcgaagc agtcgtggag ctcgatgaga tcgatgtcgt
     1621 ccgggccgag cccggccatc tcgtaggctg ccgcggccgc cttgcgggtg caggagttga
     1681 cgtccggcat gaccaggtcg cgctcctgcc atggatccga ggtgagcacg gaggcgcgaa
     1741 ccttcacggc ccgcttcatc agacccagct ccttggcctt cgcctcggag cagatcaccg
     1801 cagcggcgga gccgtccacg ttcaccgagc acatcagctt ggtgttgggg taggagatca
     1861 tctcggcgtt catcaccgtc tccagcggcg tctcgatctg gtacaaggcc ttcgggttca
     1921 tggtggagtg gtgatggttc ttcaccgaca ccttggcgaa ctgctcgaag gtggtgccgt
     1981 acttgcgcgt gtgctccatg ccggcttccg cgaacacggc cggcatggtg ccggagccca
     2041 agaggccctc cttgggaatg ccggtgccgc cgccggcccc gcccagcaga cccttgccca
     2101 tctgctcgac gcctacggcg agaacgcagt cgtaaagccc ggccttgacg gatgcccagg
     2161 cttccctgaa ggcagtggcg ccggtggcgc aggcgttgga gacgttgacg acgggtatgc
     2221 ccgtctggcc gatctgctgc aggatgcgct ggccgaccat gccgctggcc tgacccaggt
     2281 tgccgcagta aagcgcctcc atgtcctgga tggtgagccc gcaatcgtct aaggccatca
     2341 gcgcggattc ggcgccgatc tctgggaccg tcttctcggg gaagcggccg aacttgatca
     2401 tgtcgacacc gactacgtat gcgtctgtca tggagatcct cctattcgaa tcgggtcagg
     2461 aactgggttg aaaaaagaaa gacaggtacg agttgccgtc cctgtccttg cgccctagcg
     2521 cgtcggcgaa gacgacgtcc accggcatgc cgaactcgat cttgtccggg tcgggctcga
     2581 cgttgatcag gttgcctttg atcgcggtcc cgtcgtctaa gtcgaccaca gcgctcacgt
     2641 agggcacgtc gatgcccggg aacgagcggt ggacgatgga gtaggaatac agggtgccgt
     2701 ggttcggcag cgtcacggcc gacatctggt cacgggcccc gcacttggag cacacgctgc
     2761 gctcgcccaa gaacgtggcg ccgcagctgc cgcacttgta accctccagg tagggatcgc
     2821 cgtcctccgg aatcttcaga tattccacga cgggcagtgg tgtgtcgctc aaagtgtccc
     2881 ccataaactc ttgtggccca gccccgggag gatactattg acggccccct ggcgtcacct
     2941 ggcgtgggaa ggggttttgc agcaatgtac tgaagagccg tttcagcggc cagcggatca
     3001 actggggaga gccggtgaag tcagtcaaag tcgcggatta catgtccacg cgtctcatca
     3061 cgttcaaccg caccgacagc gtggtggagg ccatggccgt cctgctgaag aagaagattt
     3121 ccggcgcgcc ggtggtggac gacgagggca acctcatcgg tatcctgtct gagacagacc
     3181 tgatggcggt ggtcatccag gacagctact acgacgaggg cgtgggcatc gtgggcgact
     3241 acatgcgcga ggaggtggat accgtcgacc cggattcgga catctacagc cttgcggacc
     3301 ggtttcacaa agagggcagg cggcgcttcc ccgtggtcaa gaacggccgc ctggtcggcc
     3361 agatcagccg ccgggacgtg ctgcgggcgg cggaggaatt cctcatgcat gccgacgggc
     3421 tgagcgagcg gaagcgaagg aagcttctga cctagccggt gtcggctgcc agggccttgc
     3481 ggaggagctc gttcacctgc ttggggttcg ccttgccctg ggtcgccttc atgacctgcc
     3541 cgacgaagaa acccagcacc ttctctttgc ccgcgcggaa ctgggcgacc tgatccgggt
     3601 tctccgccac gatcccggcg atgatggcct cgatctcgcc ggcatcgctc acctgggtga
     3661 ggccgcgggc agcaatgatc tcgtccacct cgccttctcc ggcccacagc ccctcgaaca
     3721 ccgtcttggc gatcttgccg gagatggtct cgtcccggat gcggccgatg aggtcgccga
     3781 gctgtgaagg cgagatgggc gatcgggcaa tgccgagctc ttcccggttg agcgccgcgc
     3841 tgagatcgcc cattacccag ttggcggcga gcttggcgtc gccggcggag gcggcggccg
     3901 cctcgaagta gtcggcgact tccggatcct gggtgagcca ggccgcgtcg tagtcgccga
     3961 gctgaaactc ggcggcgtag cgggcgcgtc gggcatcggg cagttccggc atggtcgagc
     4021 gcaccgactc gatgaacgct tcgctgacgt gcacgggcag caggtcgggg tccggaaagt
     4081 agcggtagtc ctcggaatat tccttggtgc gcattggcct ggtctcgtgg ctgtccggat
     4141 cgtagagaag cgtctgctgc tcgatggcgc cgccggcttc gagaacgtcg atctgccgct
     4201 cgatctcgaa ctcgatggcg cgctcgagaa aacggaaaga gttgacgttc ttgatctccg
     4261 tgcgctggcc cagagcggtc tcgccggcgg ggcgcaccga gacgttggcg tcgcagcgca
     4321 tgctgccttc ggccaggttg ccgtcacaga ttttcaggtg gcgcaccagg ctgtgcagct
     4381 ttctgaagta tgcgcccgcc tcctccggcg tgcgcagctc gggttccgag accacctcga
     4441 gaagcggggt gccggcccgg ttcagatcga tgccggtcaa accggcgtac gcgtcgtgaa
     4501 tggacttacc ggcgtcctct tccaggtggg cccgcgtgat gccgatggtc cggggcgctt
     4561 cgccggaggc ttcgatggtg atcgcgcccc ggccgacgat gggatgaggc agctggctga
     4621 tctgatagcc cttgggaagg tccgggtaga agtagttctt ccggtcgaac accgagtagg
     4681 gtcggatctc cgccccgacg gcgaggccga acgtcacggc catgcgcacg gcttccgcgt
     4741 tcagaacggg aagcaccccg ggcaggccca gatccacagg gcaggcctgg gtgttcggct
     4801 ccgcgccgaa ccgggtcgag gccccggaga atatcttgct tctcgtggcc agctggacgt
     4861 gcacctcgag cccgatgacg gcctcccaac cctggggaat ccggaagccc atctcagccg
     4921 gcctccggat gccgggtgtg ccagtcggtc tcgcgctgat aggccgcgcc cgcctgcagg
     4981 agggtctttt ccgcgaagtg cggcccgatg agctgcacgc cgatgggaag cccctggtga
     5041 aaaccgcacg gcagggacat ggccggcagg ccggcaaggc tcgccggtat cgtgaagatg
     5101 tcctgctggt acatggcgac gggatcgtgg gtcagctcgc cgagcgggaa ggccacgccg
     5161 ggggcggtgg gcgcgaggat catgtccacg gtctcgaacg cctcgaggaa gtccctctgg
     5221 atcagccgcc gcagccgctg agccttcacg tagtaggcgt cgtagtagcc cacggaaagg
     5281 gcataggtgc cggtgagtat gcgtcgcttc acctccgcgc cgaacccctc ggagcgcgag
     5341 cgcctgtata ggtcctccag tgagcccgga ttctcgcagc gatagccgaa acgcacgccg
     5401 tcgtagcgcg acaggttggt ggacgcctcc gcgccggcaa tgatgtagta ggcgggaatc
     5461 gccgcgctgg tatggggcag cgagacttcc tcgagcgagc agccgatgct ttcgaagaag
     5521 ccggccacag cgtccagcag ctccgagccc gtggaaagat ccgcgaagta ttcccgcggc
     5581 agcccgatcc gcagaccgcc ttccagattt ccgacgccgt tctcggcgac cccgtcgagc
     5641 cactcgtcaa cgtgctcgct gcaggtggaa tcccgcgggt ccgcgccggc gcagaaggcc
     5701 agggacaggg caagatcctc ggcgcttctg cccaggaaac ctccctggtc caggctcgac
     5761 gcgaacgcca ccatgccgta gcggggcacg cggccgtagg tgggcttgat gccggaaacg
     5821 ccgcagaagg cggacggctg acggatggag ccgccggtgt ccgtgccgga ggccacgggc
     5881 acgaggcccg cggcgactgc ggcggccgaa ccgccggagg atccgcccgg tactcggcct
     5941 gggtcccagg gattgcgcac cgggccgaag cggctgttct cgttggacga gcccatggcg
     6001 aactcgtcca tgttggcctt gccgacggtg accgccccgg ccgcgttcag gcgttccacc
     6061 accgtcgcgt cgtagggtgc tacgaagttc tcgagcatct tcgaggcgca ggacgtgatc
     6121 gtgccccggg tgcagaagat gtccttgtgc gccaggggga tgccggtgag ggccgtggcc
     6181 gtgcccgctc tcagccgcgc atcggcagct tcggccgcgg cgagcgcgcc ctcctcgtcg
     6241 acgcagatga aggcgttcag gtcggcatgt tcccgggcgc ggtcgacaaa gaaacgggtg
     6301 agctcaacgc tcgagtagct gccgtctcgc agccgggccg cgtgctgccc gatagaagca
     6361 aacgggtcca cgttactcga cgactcgggg tacgaggtac aggccgtccc gggtggccgg
     6421 cgcgccgcgc tggtagtgat cgcggtcgac ggtctcggag acggtgtcgg gtctcagacg
     6481 ctgatccgac tcgatcggat gggccaacgg ctcgacgccg tcggtgtcca tggcctgcat
     6541 ctcgtctatc atggcgatga tgcgctgcag atcggcgagc gcggcggcct gttccgactc
     6601 cgtaagggcc agctgggcaa ggcggcagag gtgggcgatt tcgatgctgg ccatggcgcg
     6661 aagcgtaatg gaaagcggtc actgaagcca ttttccggtg cccgggaggg tgtgccgggc
     6721 agttcccgtt gccgttgcaa attgatagag taccgtccgc cgagatcccc cctcttgtca
     6781 ggggatcaag tgttcagtca attaacccga gtccttattc cagatgttta aaaatattcg
     6841 cgggctgttc tcccgggacc tgtccatcga cctgggcacc gccaacacgc tcatctacgt
     6901 cagggatcag ggcatcgtgc tgaacgaacc ttccgtcgtc gccatccggg tgcagggcaa
     6961 ccagaagagc gtggccgcgg tagggctgga tgcgaagcgc atgctgggac gcacaccggg
     7021 caacatcacc gccatccggc ccctgaagga cggcgtcatc gcagactttc tcgtcaccga
     7081 gaagatgctc aagtacttca ttgccaaggt ccacgagaac tcgttcatcc gtcccagtcc
     7141 ccgggtgctg gtctgcgtgc cctgtaaatc caccgaggtc gagcggcgcg ccatccgcga
     7201 aagcgcgctt tccgccggcg cgcgggacgt gcgtcttctc gaggagccca tggcggccgc
     7261 catcggcgca ggcctgccgg tgcaggaggc ggtggggacc atggtggtgg acatcggcgg
     7321 cggcacgacg gagatcgcca tcatctcgct gaacggcgtg gtgtactcgg ataccgtgcg
     7381 ggtcggtgga gaccgctttg acgaatccat cgtggcctac gtgcgccgta cccagggcag
     7441 cctcatcggc gaagccacgg cggagcgcat caagcaggag atcggggccg cctacccgca
     7501 gaaagaggtg cgcgagatcg acgttcgcgg ccgcaactta gcggaagggg tgccgcgcag
     7561 cttcacgctg aacagcaacg agatcttgga agcgctgcag gaacccctgt cgatgatcgt
     7621 gcaggctctg aagagcgccc tggagcagtg tccgcccgag ctcgcctcgg acatcgcgga
     7681 aaccggcctg gtgctgacgg gcggcggcgc gctgctccgt gaccttgaca ccctcctggc
     7741 agaggagacc ggcttgccgg tgatcgtcgc cgaggatccg ctgacctgcg tcgcccgcgg
     7801 cggcggcaag gccctcgagc tcatggacaa ggctggcaac gcggacctgc tgtcgaccga
     7861 atagggagac tcaagccatc aaagcgctgt ttgttcgaga atctggcgct ggcttcctgc
     7921 tcaccgggct cgtcctcgta tccctgatac tggtcaccct ggacctcacc acctctttca
     7981 tgcgcggcac ccgccaggtg ttcggcacca tgctcgctcc ccttcactac gtggcggaaa
     8041 cgccgtacct ggtgggagag gagctcggca acgtgttcgc gacccgctcc gcgctgctcg
     8101 atgaaaacca gatgctcaag cgtcaggttc tggagctttc gaagatctcg ctgcagtacc
     8161 ggaaccttct caaggagaac gagaatctca gggaactgct cggcagccgg gccaggctcc
     8221 cggacgaggt gctcattgcc gagatcatcg ggatcgtgcc gacggtaggc gcccatcagg
     8281 tggtgatcga caagggctcg gacgccggcg ttcagattgg ccaggccgtc atcgactccg
     8341 aagggctgtt cggtcaggtg gtggaggtgg acgtctattc cagccgggtg ctgctcatca
     8401 cggaccggga tcacgcggtg cccgtgcaga tcaaccgtaa cggcattcgc agcatcgccg
     8461 gcggcacggg ccggatggac cagctggagc tggagaacgt gaccatcacg gcggacgtcg
     8521 tgaagggtga tctggtggaa acctcgggac tcggaggccg ttttcccgag gggtacccag
     8581 tgggggaggt ggagtcggtc gtggtggatc cggcggcgag cctgtcccgg gtgctggtgc
     8641 ggccgtcggc gctgctggat cgatcgcgac acgttctggt ggtgttcgcg cccgagaccc
     8701 gtgacgaggt gttgccgtga accctgctca gggcgggtgg ctcatcctcg caagcctggt
     8761 gattgccctg gtgctttcca tctttcatct gccggagacg tggccgcagt ggctgggctg
     8821 gctccggccg gcttgggtac cgctgttcgt cttcttctgg gtgatggcgc tgccccatcg
     8881 gctggggctg gtatccgcct ggatcttcgg gatcttcgtc gacgtgctgt tcggggatcc
     8941 catcggcctc aacggcgcgg tgctggcggg catcacctac atcggctggc gcttctacga
     9001 gcgcctgcga atgtactccg tggttcagca gtgcggggtg gtcttcctgc tgatcctcgg
     9061 cggtgatctg gtgcgcatgc tggtgcacga cctttccgcc gatcgcggct ggagctgggc
     9121 cgtgctggtc cccgcgctca cgagcatgct ggtctggccg ttcgtctccc tgtttctgca
     9181 gcgcctgcgg ctgcaattcg gcgtcgagtg agtccggcgt cggtgccagc cggccgttcg
     9241 gcgagccatt cggcgagcca ttcggcaagc cattcggcga gccacatcgt gctggcttcc
     9301 gggtcgccgc gccgcgccga gctgcttcgt cagatcggcc ttcccttcgt cgtccgcccg
     9361 ccggacgtgg acgagtcgct tcgcccgggt gagtcccccg agcgatacgt ggagcgcctc
     9421 gcgatcggca aggcgcgggc ggtggtggca gaggacgggc cggtcctcgg agccgatacc
     9481 gccgtggtcg tggatgagga gatcctcggc aagccggcgg atcgggcatc ggccatcgac
     9541 atgatcctgc gcctctccgg acgcacccat cgggtgctca cgggggtggc ggtgaccgac
     9601 gggcgggccg tgcggtcctg cgtggtggcc gctgacgtga cgtttcgggc catcacccgc
     9661 gatgaggctg ccgcgtactg ggagacgggc gagccgcgcg acaaggcggg cggttacggt
     9721 attcagggga tcgggggtat atttgcgcaa agcatcaacg gcagctacag cgcggtggtc
     9781 ggcttgcccc tggcggagac cgaagcgctg cttcacgcca ttggcgtgaa cacgtgggag
     9841 ctgcgggcaa gtgtctgaag aactgctcat caacgtcaac gacttcgagt cccgggttgc
     9901 gctggtgtcg gggtccgtcg tcgaggaggt gcaggtcgcc cgggccggcg cagacagcct
     9961 gaccggcaac atctatctgg gtcgcgtcca gcgcatcatt ccggggatgc aggccgcgtt
    10021 cgtcgactgt ggcctggaac gccccggctt cctgcacgtc agcgacatcg caggcccccg
    10081 gctgatgctc ggcgaagagc cggacggggc ggtcg
//
LOCUS       DBNHKV010000110         2223 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_228756, whole genome shotgun sequence.
ACCESSION   DBNHKV010000110 DBNHKV010000000
VERSION     DBNHKV010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_228756"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="AC16PP_03560"
     CDS             <1..735
                     /locus_tag="AC16PP_03560"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006720 - isoprenoid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6480731.1"
                     /translation="IFPIMPLTGSMERAMQGGPVYAWEPDKGVHIGIMPRNGSVDELR
                     WFKGDPSFVFHPMNAHSDGNMVTCDVCEFEEAPLFPAPDGTPGDPDKAVPRLTRWTFD
                     LGSNTDDYSSERLDDLACEFPRLDERFTGLGYRHGYFACDLKPEFQVGAFNGIGHVDH
                     ATGKIDVYAVGQACATNEPIFVPRTEDSAEGDGFLLANVYDAERKASHLVILDAQNVS
                     AGPLAKAYLDHRVPFGFHGNWMPGQA"
     gene            765..1898
                     /locus_tag="AC16PP_03565"
     CDS             765..1898
                     /locus_tag="AC16PP_03565"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR6480732.1"
                     /translation="MLRRVLATVFLCTALPLCHAAMTAADLGRLLFFDANLSLQRTQS
                     CASCHDESRAFSDGRDNGVRGAASLGDDGRSLGDRNAPALTYVALTPPFRIRGEGQPA
                     GGFFLDGRVATLQEQAAEPFVNPLEMQMPDPTAVVARVRENPTYERSLRRLFGEAVFE
                     DTDTAYRAVGESLAAFEQADELASFDSRYDRHLRGEYQMSEQEALGRDLFFSQLTNCS
                     LCHALSPHATNDARETFTDYGFRNIGVPANAHLRSANGTKPGHTDRGLLENPAVERAE
                     HAGKFKVPTLRNVAVTAPYMHNGVFRELRTAIHFYNRYLVRNPASMTNPETGEPWAPA
                     EVEETVDLALLREGQPLSDERIALIEAFLRTLTDERYEHLLEK"
     gene            1978..>2223
                     /locus_tag="AC16PP_03570"
     CDS             1978..>2223
                     /locus_tag="AC16PP_03570"
                     /inference="COORDINATES: protein motif:HMM:NF023594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6480733.1"
                     /translation="MTSVANQEPIAEGLFTWPSNDPRLLGSRCDDCGVVTFPAQNACP
                     ACCGEDTTTIELERRGTLWTWTIQGFLPNRPPYDGPET"
BASE COUNT          393 a          792 c          692 g          346 t
ORIGIN      
        1 atcttcccca tcatgcccct gaccggatcc atggagcggg ccatgcaggg gggacccgtc
       61 tacgcctggg agccagacaa gggcgtccac atcggcatca tgccgcgcaa cggcagcgtg
      121 gatgagctgc gctggttcaa gggggatccc tcgttcgtct ttcaccccat gaacgcgcac
      181 agcgacggca acatggtgac ctgcgacgtc tgcgagttcg aggaggcccc tctcttcccg
      241 gcacccgacg gcaccccggg cgaccccgac aaggcagtcc cccggctcac ccgatggacg
      301 ttcgacctgg gcagcaacac cgacgactac tcaagcgagc ggctcgacga tctcgcctgc
      361 gagttcccgc gactggatga gcgcttcacc gggctcggct accggcacgg ctacttcgcc
      421 tgcgacctga agcccgagtt ccaggttggc gcgttcaacg gtatcggcca tgtggaccac
      481 gccacgggca agatcgacgt ctacgccgtg gggcaggcct gcgcgaccaa cgagccgatc
      541 ttcgtgcccc gcaccgagga ctcggcggag ggtgacggct ttctgctcgc caacgtctac
      601 gacgctgagc gcaaagccag ccacctcgtg attctcgatg cccagaacgt gagcgccggg
      661 ccgctggcca aggcctacct ggatcaccgg gtacccttcg gcttccacgg caactggatg
      721 ccgggccagg cctgagcacg gctcggcccg acccggaagg ctcgatgctc aggcgcgtgc
      781 tggcgacggt tttcctgtgc acagcgttgc cgctctgcca cgcggcgatg acggccgccg
      841 atctcggccg actgctgttc ttcgacgcca acctctcact gcagcggacc cagtcctgcg
      901 ccagctgcca tgacgagtcc cgggcgtttt ccgacggtcg cgacaacggc gtgagaggcg
      961 cggcctccct gggagacgac gggcggtctt tgggcgaccg gaacgcccca gcactgacct
     1021 acgtcgcctt gacgccgccg ttccggatcc gcggcgaggg ccaaccagcg ggcggcttct
     1081 tcctcgacgg gcgcgtcgcc accctgcagg aacaggccgc ggagccgttc gtgaacccgc
     1141 tggagatgca gatgcccgat cccaccgccg tggtggcccg ggtgcgggaa aacccgacct
     1201 acgagcgttc cttgcggcgg ctgttcggag aggcggtatt cgaggacacc gacacggcct
     1261 accgcgccgt tggcgaaagc ctcgccgcct tcgagcaggc ggacgagctc gccagcttcg
     1321 attcccgcta cgaccggcat ctgcggggcg agtatcagat gagcgagcag gaggccctcg
     1381 gccgggatct gttcttctct cagctaacca actgcagcct ctgccacgcg ctcagccccc
     1441 acgccaccaa cgacgcgcgg gagacgttca cggactacgg gttccgcaac atcggcgtgc
     1501 cggccaacgc ccacctgcgc agcgcgaacg gcacgaaacc cggacacacg gaccgcggcc
     1561 tgctcgaaaa cccggccgtc gaacgcgcgg agcacgccgg caagttcaag gtgcccactc
     1621 tacgcaatgt ggcggtgacg gccccgtaca tgcacaacgg ggtgtttcgc gagctccgca
     1681 cggccattca tttctacaac cgctatctgg tgcgcaaccc cgcgagcatg accaatccgg
     1741 agaccggcga accgtgggcg ccagccgagg tggaggaaac cgtcgacctg gccctgcttc
     1801 gcgagggcca gccgctatct gatgagcgta ttgcgctcat cgaagcgttt ctgcgcacgc
     1861 tgaccgacga gcgatacgag cacctgctcg agaaatgagc ccgcttcccg gccacccggt
     1921 gacaaacgcc ctccggctct gctacaaacc atgttcacat ctttcgggaa agcttaagtg
     1981 accagtgttg ccaaccagga acccatcgcc gaggggctgt tcacctggcc atcgaacgat
     2041 ccgcgcctgc tcgggagccg ctgcgacgat tgcggcgtgg tgaccttccc cgcgcagaat
     2101 gcctgccccg cctgctgcgg tgaggacacg acgacgattg agctcgagcg tcggggcacc
     2161 ctgtggacct ggaccattca gggctttctt cccaaccgcc caccctacga cggccccgag
     2221 acg
//
LOCUS       DBNHKV010000111         2349 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1575062, whole genome shotgun sequence.
ACCESSION   DBNHKV010000111 DBNHKV010000000
VERSION     DBNHKV010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1575062"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="AC16PP_03575"
     CDS             complement(<1..1237)
                     /locus_tag="AC16PP_03575"
                     /inference="COORDINATES: protein motif:HMM:NF023165.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HIR6480734.1"
                     /translation="MVALLGLLTLLVAGNTARGEPAMEAVAADGSTYPVEPVALLDPA
                     ALEELVGPIALYPDDLLAIALPAATYPLQVVQASRFLQSREENPSVQPAEGWDDSVVA
                     LLNYPEVVELLNEDLDWTWKLGEAVLAQQEDVLEAVAAFRERARLAGNLRSDDFQLVE
                     SADEGIRIRPRDPEVIYVPYYEPSRVTAYHSRPVYHYYPARYPVYYYPYAPGHRFASG
                     FWGVTSAFSIGWTTRRLHVHYVTYRDHPYYGHHYYDRYFYRRPPSFRVDDHWQWHGSF
                     AGLGRHHAGNYWYPRAHRHGPSPRHHHRRSHHEPNQDEGTDREPRRHRERERERAADR
                     PGGSEEHLADRRDPRERLVPELGPRRAGRLLARGSVVNGIPVPESAATLAPRPARPPA
                     AADSPGSAQRRPGSPIEAQQ"
     gene            complement(1462..>2349)
                     /locus_tag="AC16PP_03580"
     CDS             complement(1462..>2349)
                     /locus_tag="AC16PP_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR6480735.1"
                     /translation="VIYLFTRDIEHWAAHPLAMKDKMGKSSISMFDRAGNYLGKWGPS
                     DERGFALGAHTLYIEKDGMFWTTDRDGHTVRKYHPDGTLLLTLGEFGQWGAGTDHFNG
                     PTAVLVQENGNIVVSDGYWNSRLVWFSPEGEFIREVGTWGTGPAEFGTPHAIAQDAQG
                     RILVADLAGGNFHPYMTLPGQIAEHRTRQIPDAKSRIQILDPDGAYIGEWDHIMPLSL
                     AVYGDHVYASDKMSNLAILDAATGKVEERVDNIAIYIHQLAMDAHGDIYTASVYPEHA
                     GEKRGPEGPSHRRWTRDGA"
BASE COUNT          387 a          765 c          798 g          399 t
ORIGIN      
        1 cctgctgcgc ctcaatgggt gagccgggcc ggcgctgggc cgaccccggg gaatcggcag
       61 ccgcgggcgg ccgcgcgggt cgcggcgcca gcgtggccgc cgactcgggc accggaatgc
      121 cgttcaccac cgatcccctc gccaacagac ggccagcgcg gcggggtcca agctccggga
      181 cgagccgttc ccgcgggtcg cgccggtcgg cgaggtgctc ctcggaaccc ccggggcggt
      241 cagcggctct ttcccgctcc cgctcccgat gtcgtcgggg ctcgcgatcc gtcccctcat
      301 cctggttcgg ttcgtgatgg cttcgtcggt gatggtgccg cgggctcggg ccgtgacgat
      361 gtgcacgcgg ataccagtag ttgcctgcat gatggcgtcc cagcccggca aagctgccgt
      421 gccactgcca gtggtcgtcg acccggaagg acggtggccg acggtagaag tatcgatcgt
      481 aataatgatg gccgtagtag ggatggtccc ggtaggtcac gtaatgcacg tgaagccgcc
      541 tggtggtcca gccgatgctg aatgccgagg tcacgcccca gaacccgctc gcaaatcgat
      601 gtccgggcgc ataggggtag tagtaaacgg gataacgagc cgggtagtag tgatacaccg
      661 ggcgggagtg gtaggcggtc accctggaag gttcgtagta gggaacgtag atcacctcgg
      721 gatcccgcgg ccggatccgg ataccctcgt ccgcgctctc caccagctgg aagtcgtcgc
      781 tgcgcaggtt gccggcaagc cgggcgcgtt cccgaaacgc cgcgaccgcc tcgagcacat
      841 cctcctgctg ggcgagcacg gcctcgccga gcttccaggt ccagtccaga tcttcgttca
      901 gcagctccac cacctcgggg tagttgagga gcgccaccac cgagtcgtcc cacccctcag
      961 ccggctgcac ggaagggttc tcctcgcgcg attgcagaaa ccgagaagcc tgcacgacct
     1021 gcagcggata ggtcgcggcg ggcagggcaa tagccagaag gtcgtcggga tagagggcga
     1081 tgggcccgac cagttcctcc agggccgccg gatccaggag cgctaccggc tccaccgggt
     1141 aggtgcttcc gtccgcggcc acggcttcca tggccggctc cccacgggcc gtgttgcctg
     1201 ccacgagcag ggtgagcaac ccgagcaagg cgaccacccg ggcccgctgc cggacccagg
     1261 acgttgagca atggaaccct gtaatcatcc gatgcctctc agcttcctga tccgcgtggc
     1321 gaggcagtcc ctggcgttct ttccactcat atcggacgag actatccccg tcaacctgaa
     1381 cgggacatga agggcagccg gtcaaccagg gggcggggcg gacgggcggt ccgcgcagga
     1441 aaggatcaga ttccttcggg atcaggcgcc gtcgcgggtc cagcgccggt gggacggacc
     1501 ctcggggccg cgcttctcgc cggcgtgctc cgggtagacg gatgccgtgt agatatcacc
     1561 gtgggcgtcc atcgccagct gatggatgta gatggcgatg ttgtcgacac gctcttccac
     1621 cttgccggtg gccgcatcca gaatcgcgag gttgctcatc ttgtcgctgg cgtagacgtg
     1681 atcgccgtag acggcgaggc tcagcggcat gatgtgatcc cactcgccga tgtacgcgcc
     1741 gtccgggtcg aggatctgaa tacggctctt ggcgtcggga atctgccggg tccgatgctc
     1801 ggcgatctgg ccgggcagcg tcatgtaggg atgaaagttg ccgccggcga ggtcggccac
     1861 cagtatccga ccctgggcgt cctgagcgat ggcgtgaggc gtgccgaact ccgccggccc
     1921 agtgccccag gtgcccacct cgcggatgaa ctcgccctcg ggcgaaaacc acaccagccg
     1981 ggagttccag tagccgtccg acaccacgat gttgccgttt tcctgcacga gcacggccgt
     2041 ggggccgttg aaatggtcgg tccccgcacc ccactggccg aactcgccga gggtcagcag
     2101 cagggttcca tcgggatgat atttgcgcac cgtgtggccg tcacggtccg tggtccagaa
     2161 catgccgtcc ttttcgatgt agagcgtatg ggcgccaagc gcgaagccgc gctcgtccga
     2221 ggggccccac ttgccgaggt agttgcccgc gcgatcgaac atggagatgc tgctcttgcc
     2281 catcttgtcc ttcatggcca ggggatgagc ggcccagtgc tcgatgtccc gggtgaacag
     2341 atagatcac
//
LOCUS       DBNHKV010000112         2390 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1856871, whole genome shotgun sequence.
ACCESSION   DBNHKV010000112 DBNHKV010000000
VERSION     DBNHKV010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1856871"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="AC16PP_03585"
     CDS             complement(<1..1253)
                     /locus_tag="AC16PP_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015635058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR6480736.1"
                     /translation="MNHASALRQLRAALPEGAVLTDRHQLKPYETDGLTALRQMPWAV
                     ALPQTEAEVREVLRICAEHEVPLVPRGAGTGLSGGARPVSDGLVLGVSRLDRILEIDV
                     ENGVARVEPGVRNLAISEAASAYGLYYAPDPSSQIACSIGGNVAENSGGVHCLKYGLT
                     VHNVLGLKVLTVEGDELQIGGKRLDSPGYDLLALMSGSEGMLAVITEITVRLLPIPPE
                     KRVLLGAFRTVAAAAEAVNQVISEGLVPAGLEMMDRLAIEAAEDFVHAGYPREAEAIL
                     LCEVDGVTEEVDNDVDLVRRVFETLGAFDVRIATDPAEQANLWKGRKAAFPAVGRISP
                     DYYCMDGTIPRYELARVLAEIASLATEFGLRVANVFHAGDGNLHPLILYDAGREGELA
                     KAEALGGKILELCVEVGGTVTGEHGV"
     gene            1388..2332
                     /locus_tag="AC16PP_03590"
     CDS             1388..2332
                     /locus_tag="AC16PP_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024294.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6480737.1"
                     /translation="MTNSYLISNARIVNEGTITEGDLLVENGRISGIGKPAPAGTDVF
                     DANGAWLLPGMIDDQVHFREPGFEHKGTIATESRAAIAGGITSYMEMPNCNPLTVTHE
                     ALADKHVRAKDHSAANFAFYFGATNDNLEAIKTVDPALTCGIKVFMGASTGNMLVDDA
                     DTLEGIFAGTPLIVATHCEDTPTILANEERAREAFGDAIPFSEHPRIRSEEACWLSSS
                     MAVGLARRHGTRLHVLHLTTAREMALFEPGDLMSKQITAEACVHHLFFNESWYPDKGA
                     DIKCNPAIKGAADQGITWRCSRAVLTLAHCSIGSTTAP"
BASE COUNT          465 a          827 c          716 g          382 t
ORIGIN      
        1 acaccgtgct ctccggtcac cgtgccgccc acctcgacgc aaagctcgag gatcttgccg
       61 ccaagggcct cggctttcgc gagctcacct tcgcgccccg cgtcgtaaag aatcaatggg
      121 tgcagattgc cgtcgccggc atggaagacg ttggcgactc gaagcccgaa ctccgttgcc
      181 agcgaagcga tctcagccag cacgcgcgcg agctcatagc ggggtatggt tccgtccatg
      241 cagtagtagt ccggagagat acgccccacc gcgggaaagg cggctttgcg gcccttccac
      301 agattggcct gctcggccgg atcagtggcg atgcgcacgt cgaaagcacc aagcgtttcg
      361 aacaccctgc gaaccaggtc gacgtcgttg tccacctcct cggtcacgcc gtcgacctcg
      421 cacagcagaa tggcttccgc ctcccgcggg taaccggcgt gcacgaagtc ctcggccgcc
      481 tcgatggcga gccggtccat catctccaat cctgccggca ccagaccctc tgagataacc
      541 tgattcacgg cctccgccgc cgctgcgacc gtgcggaacg ccccgagcag cacccgcttt
      601 tccggcggta tcggcaggag ccgcacggta atctcggtga tcaccgcgag catgccttcc
      661 gagcccgaca tcagcgcgag cagatcgtaa ccgggagagt ctaggcgctt accgccgatc
      721 tgcagctcgt cgccttcgac ggtgagcacc ttcagcccca aaacgttgtg aacggtgagg
      781 ccgtacttca ggcaatgaac gccgccggag ttttccgcca cgttgccgcc gatgctgcag
      841 gcgatctggg aggaagggtc gggcgcgtag taaagcccgt aagcggatgc tgcctcgctg
      901 atggccaggt tgcggacgcc tggctcgacc cgggccacac cgttttccac gtcgatttcg
      961 agaattcgat ccaaccggga aacccccagc accaggccgt cgctgacggg cctcgccccg
     1021 ccggagagcc cggtcccggc cccccgcgga accaaaggca cctcgtgctc ggcgcagatt
     1081 cgcagcacct cccgcacctc tgcctcggtc tgtggaagcg ccacggccca gggcatctgc
     1141 ctgagcgcgg tgagcccgtc cgtttcgtag ggcttgagct ggtgccgatc ggtgagaacg
     1201 gctccctcgg gcagggcggc acgcagctgt cggagagcgc tggcatggtt catggtggag
     1261 gagtatacgc catggaagac ggcgtctttc acgtcggcga gcgcatcctg cccgccaaat
     1321 cggctaaagt aaccccaacg cagaaaccgc ccacgggaag caccgcgcgg cgaacggacc
     1381 agacttcatg accaacagct atctcatcag caacgcccgg atcgtcaatg aggggaccat
     1441 cacggaaggt gatctcctcg tcgagaacgg ccgaatctcc ggcatcggca aaccggcacc
     1501 ggcaggcacc gacgttttcg acgccaacgg cgcctggctc ctgcccggga tgatcgacga
     1561 ccaggtgcac tttcgagagc ccggtttcga gcacaaggga accatcgcca cggaatcccg
     1621 cgcggccatt gccggaggca taacgagcta catggagatg ccgaactgca acccgctgac
     1681 ggttactcat gaggcgctgg ccgacaagca cgttcgcgca aaggaccatt ccgccgcgaa
     1741 tttcgccttc tactttggcg ccaccaacga caacctggag gccatcaaga ccgtggatcc
     1801 cgccctcacc tgcggaatca aggtcttcat gggggcgagc accggcaaca tgctggtgga
     1861 cgacgccgac accttagagg gcatcttcgc cggcacgccg ctgatcgtgg ccacccactg
     1921 cgaggacacg cccaccatcc tggccaacga ggaacgcgcc agggaagcgt tcggcgacgc
     1981 catcccgttc tcggagcacc ctcgaataag gtccgaggaa gcctgctggc tgtcatcgtc
     2041 catggccgtc ggtctcgccc gcagacacgg cacccggctc cacgtgctgc acctgaccac
     2101 ggcgcgcgag atggcgcttt tcgagccggg agacctgatg agcaagcaga tcacggccga
     2161 agcctgcgtg caccacctct ttttcaacga gagctggtac cccgacaagg gtgccgacat
     2221 caagtgcaac cctgccatca agggcgccgc tgaccagggg atcacctggc gctgttcacg
     2281 agcggtcttg actttggccc actgcagcat cggttcgacc acagcaccgt aacgaccccg
     2341 ctcgcgcggc gcccacaggc gctgaacgta ctcggccagc gattgatacg
//
LOCUS       DBNHKV010000113         2024 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1220817, whole genome shotgun sequence.
ACCESSION   DBNHKV010000113 DBNHKV010000000
VERSION     DBNHKV010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1220817"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="AC16PP_03595"
     CDS             <1..202
                     /locus_tag="AC16PP_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480738.1"
                     /translation="GRLFVLIVRHRCAYLPPHVQFTEERYMKTRTTWLVGAIAITMTL
                     FAGTAFAGKTCEEQEAKDGACQ"
     gene            complement(353..1746)
                     /locus_tag="AC16PP_03600"
                     /pseudo
     CDS             complement(353..1746)
                     /locus_tag="AC16PP_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612190.1"
                     /note="programmed frameshift; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          332 a          685 c          642 g          365 t
ORIGIN      
        1 cggtcgcctg ttcgtcttga tagtccggca ccgctgcgcg taccttccgc cccacgttca
       61 gttcacagag gagagataca tgaagacacg cacgacgtgg cttgtgggag ccattgcgat
      121 cacgatgacg ctctttgccg gaacggcctt tgccgggaag acctgcgagg agcaggaagc
      181 gaaggacgga gcctgtcaat gaaagtgaga cagtcggcat cggaatttag tgtagcgccg
      241 gccgcggcga gcgcatgatc gaggcggcgg agacgctgtt gtcgggggta cctatgccct
      301 cacccgattc gagatcgcgg ctctgagggg atcctacgac gtcggttttc tcggcgtcgt
      361 ccgtgggcag gggtttgggc ggattgatcc acacctcggt gggtcgtgcc ggcggctgtg
      421 gaagtccgcg gacgaagcgc tcgggatgtg cggcgtatgc gctggccagc acctgcgcgc
      481 ggtctgcgat gcgctgatcg gccagcccgt agtggacttc gtagggcgtc agcaggccga
      541 tgccactgtg ccgatgctcg gtgttgtacc aggggaagaa gacccggcag aaagcacgcg
      601 agtccaccat cgagccaaag cgatcgggaa actgcggccg gtacttcatc gtcttgaact
      661 gcgcctcggg gaacgggttg tcgtccgaga cgtagggacg gctgtgcgac ttgatgacgc
      721 ccagatcggc gagcagcagc gccaccggct tcgaggtcat cgaggagccg cgatcggcat
      781 gaatggtgag ctgtcccggc acgatgccct ggcgtgcaca ggtgtcgcgg atcagcttct
      841 cggccaacgc cgcactctca cggtgtgcca ccatccaacc caccacgtag cggctgaaca
      901 cgtcgagaat cacgtacagg tagaagtagg tccacttcgc cgggccgaga agcttggtga
      961 tatcccaact ccagagctgg ttgggcgcgg tggccagaag ttgtggagcg gcgtagcggg
     1021 ggtggcgcag ttgatcacgc cgctcccgca cctcctggtt cgcttccagg atgcggtaca
     1081 tcgtgcgttg cgagcagacg tagcgccctt catcgagcag gccggcgtac acctgcgccg
     1141 gggcgagatc gacaaagcgg ggctcatgca ggatgtccag caccgccctc cgctccgccg
     1201 caggcagggt ccgcggcggg ctcgggcgag atgcggccgc ggccggctgc ggtttgcatc
     1261 gccggtagta gctggccgtg gagacgccca gcgccgcgca ggtcggcgcg actcccaggc
     1321 gcgctccaac ctctttcacc gtcgccatca ggatctctcg ttgtccaacg gcgtccccag
     1381 caacgccgcc actttttttt gaacttctac cagtgcctcg gcgcgctctg cgcgcttacg
     1441 ccacttcccg agctcgcgct cctgctccgc gattttcttg tcgcgggggt ccggcggcac
     1501 tcctttcggc ccccggcgct tcggggccag cccatcgagt tccccccgat cgcgcgccgc
     1561 ccgccacgtc gtcagcgacg acgagtacag cccctcgcgc cgcagcaacg ccccgatcgc
     1621 ccccggcgtg gtgcagcggt ccgcctcgcg cacgatccgc cgcttgtact cggccgtgaa
     1681 ccgccgccgc ttcggcttcg ctaccacttc ggtctccggc gctgattccg caccgacggg
     1741 cgccagcggc gccgcgctcg gggaaactcc actcgcccta cgggctcctt ccgtttcccc
     1801 gagccattca cccaccacgt tcttcccaac agccttcatc catgtctgcc tccccgccct
     1861 ctacagtaaa ctatgaccac ccaactgtct caccctcatt gacagagagg ggcggcccag
     1921 tcgaccaacg tccaccgttt cgtttcagaa cggcccaggc caagttctac gcgcttacgc
     1981 gctaaggctt ccgtgaaggg acgagcaagc ttctgcgact cgcc
//
LOCUS       DBNHKV010000114         4668 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1078924, whole genome shotgun sequence.
ACCESSION   DBNHKV010000114 DBNHKV010000000
VERSION     DBNHKV010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1078924"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..457
                     /locus_tag="AC16PP_03605"
     CDS             197..457
                     /locus_tag="AC16PP_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480739.1"
                     /translation="MPDDELKRARDDARCEAAIQTLTDVLWRFEETCEGSYTLYDLVG
                     YLVEDLVREGCCAACIAESVTAAFKDIGADTTEHRRDEGTIH"
     gene            530..1300
                     /locus_tag="AC16PP_03610"
     CDS             530..1300
                     /locus_tag="AC16PP_03610"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6480740.1"
                     /translation="MSLFRLDNKNIAITGASSGFGHHFAGVLSEAGASVVLGARREDK
                     IRDRVDEINAAGGKATAVKLDVRDADSVRGFLEAAEAAYGPLDVVINNAGVEAGAKTY
                     LMIDEDDWDFVMDTNLKGAWLVSKTYTELVLAAGRKQGNIINISSITDTRTIKGQFPY
                     AVSKGALTRMTEVMALEAAKYGIRVNALAPGYILTDVSRLLLESDQAPEFVKGIPMRR
                     FGEFSDLDGPLLLLASDASTYMTGSVVVVDGGHICASL"
     gene            1327..2466
                     /locus_tag="AC16PP_03615"
     CDS             1327..2466
                     /locus_tag="AC16PP_03615"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017255227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase family protein"
                     /protein_id="HIR6480741.1"
                     /translation="MTDVRPFRIEIPEKDLDDLGQRLALTRLPEKETPDDWSQGVPLA
                     YMREIVDYWQSDYDWRRAEEEINRFPQFTTELDGLNVHFIHVKSPEPNALPLVMTHGW
                     PGSIVEFLKVIGPLADPASHGGDPADAFHVVCPALPGYGFSGKPITPGWGVEKIGSEW
                     ANLMARLGYESYVAQGGDWGSAVTTAIGMQDPDHCRAIHLNMVVAQPDPATMDDLTDL
                     EKRGIAGMQHYQQWDSGYSKEQSTRPQTIGYSLVDSPVGQAAWIMEKFWAWTDCDGHP
                     ENALSRDEMLDNIMLYWLTASGASSARLYWESFGSFSAGDVSVPTGCSIFPKEIFTCS
                     RRWAEQRFKNIVYWNELDRGGHFAAFEQPELFVGELRNCFRQVRG"
     gene            2463..4400
                     /locus_tag="AC16PP_03620"
     CDS             2463..4400
                     /locus_tag="AC16PP_03620"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HIR6480742.1"
                     /translation="MTEGPIWRPDPVRALESNMARFTEEAARVSGRSMTGYADLHAWS
                     IEEPAAFWDLVWHFTDVRASQAPTAILTNADQLPGARWFEGARLNFAENLLRFDDDRP
                     ALIALLETGERREISYAELRRETAQVAAWLKNHIDPGDRVAGWLPNVPEAVTAMLATT
                     SLGGVWSSCSPDFGAEGALDRFGQIEPKVLFACDGYHYNGKPISTVDKARQVAERIPS
                     IRAIVWISLAGEDLVESDGVAYASLLEGDHDLAFAQRAFDDPLYVMYSSGTTGKPKCI
                     VHGIGGTLIQHLKEHQLHTDLRRDDRLFYFTTCGWMMWNWLVSGLATGATLVLYDGSP
                     FHPGPEALLDMAEQEDVTVFGVSARYIAALQKTSLKPMETHDLSALRAILSTGSPLTH
                     EGFRYVYEHIKRDVLLASISGGTDIISCFVLGNPTLPVWEGEIQCRGLGMAVEVVDDG
                     GRPVTGEKGELVCTRPFPSCPLGFWNDTDGAKFRAAYFQRFPGIWAHGDFAEVTEHDG
                     FVIHGRSDAVLNPGGVRIGTAEIYRQVEHVDAVLESLCVGQEWEGDTRVVLFVVMRPG
                     RELDEALAQQIRQRIRQHASPRHVPARIVQVADIPRTLSGKIVELAVRETIHGRPVQN
                     TSALANPEALDLYSNLPELES"
     gene            complement(4454..>4668)
                     /locus_tag="AC16PP_03625"
     CDS             complement(4454..>4668)
                     /locus_tag="AC16PP_03625"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6480743.1"
                     /translation="EDWTEEESKPLLLYLYQHASRAEFTYRFRWQPGSLALWDNRATW
                     HYAVNDYHGKRRLLHRITIEGEPLGP"
BASE COUNT          852 a         1571 c         1467 g          778 t
ORIGIN      
        1 gcgtcccgcc aaagcgggac gcaggccccg ggccggagcc gagtgtcggc cagttcaatc
       61 ctctgactcc gaccattttg actcagtggt ttgcggctac cgacgcacca ccgacgcgtc
      121 ccgctcaaag cgcgacgcaa gccccgcgcc ggccggttac aatgcccgat accgcatcat
      181 gcctggagcc caagacatgc cggacgacga actcaaacgc gcgcgcgacg acgcccgctg
      241 cgaggcggca atccagaccc tcacagacgt gctgtggcgg ttcgaggaaa cctgcgaggg
      301 ctcctacacc ctctacgacc tcgtgggcta cctggtggaa gacctggtcc gggagggctg
      361 ctgcgccgcg tgcatcgcgg agtccgtgac ggcggcgttc aaggacatcg gcgccgatac
      421 caccgagcac cggcgcgacg agggaaccat acactgacca ccgcctgcct ggcctgccac
      481 attctttgtc gctaggattg tgctcccgtc agggcaggag ggttttcgcg tgagtctgtt
      541 ccggctcgac aacaagaaca tcgccatcac tggcgcatcc tcgggattcg gccaccactt
      601 cgcaggcgtg ctttccgagg ccggggccag cgtggtgctc ggcgcccgcc gcgaagacaa
      661 gattcgcgac cgggtcgacg agatcaacgc tgccggcggc aaggcaaccg cggtaaagct
      721 ggacgtgcga gacgccgaca gcgtcagagg cttcctcgag gccgccgagg ctgcctacgg
      781 gcccctggac gtggtcatca acaacgccgg cgtcgaggct ggcgcgaaaa cctatctgat
      841 gatcgacgag gatgactggg acttcgtcat ggatacgaac ctcaaaggtg cgtggctcgt
      901 ctccaagacc tacaccgagc tcgtcctcgc ggccggtcgc aagcagggca acatcatcaa
      961 catctcatcc attaccgata cccgcacgat aaagggacag tttccgtacg ccgtctccaa
     1021 gggagcgctc acccgcatga ccgaggtcat ggccctggag gcggccaagt acgggatacg
     1081 cgtcaacgcg ctcgcgcccg gctacatcct cacggacgtg agccgcctgc tgctcgagag
     1141 cgatcaggcg cccgagttcg tgaagggcat tcccatgcgc cggttcggcg agttctcgga
     1201 cctggacggt cccctgttgc tgctggcctc cgacgcttcg acctacatga cgggatccgt
     1261 ggtcgtcgtg gacggcggcc acatctgcgc gtcgctgtga cgctgaaacc aggcaggaga
     1321 cagaccatga ccgacgtacg ccccttcagg atcgagatac ccgagaagga tctggacgac
     1381 ctgggccagc gcctggcgct gacccggctg cccgagaagg aaacgcccga cgactggagc
     1441 cagggagtgc cgctggcgta catgcgcgag atcgtcgact actggcagtc cgattacgac
     1501 tggcgacgcg ccgaggaaga gatcaatcgc ttcccgcagt tcaccaccga gctcgacggg
     1561 ctgaacgtcc acttcatcca cgtgaaatcg cccgagccga acgccctgcc cctggtaatg
     1621 acccacggct ggcccggctc catcgtcgag ttcctgaaag tgatcggccc gttggccgat
     1681 cccgccagtc acggcggcga tccggcagac gcctttcacg tggtctgccc ggcgctcccc
     1741 ggctacggct tttcgggcaa gcccatcacc ccgggctggg gggtggagaa gatcggcagc
     1801 gagtgggcaa acctaatggc ccgcctgggc tacgaaagct acgtcgccca ggggggcgac
     1861 tggggctcgg cagtcaccac cgctatcggg atgcaggatc ccgaccactg ccgcgctatt
     1921 cacctcaaca tggtggtcgc ccagccggat ccggcaacca tggacgacct gaccgacctc
     1981 gagaaaagag gcatagcggg catgcagcac taccagcagt gggactccgg ctactccaag
     2041 gagcagagca cgcgtcccca gaccatcggc tacagcctgg tcgactcacc tgtgggccag
     2101 gccgcctgga tcatggagaa gttctgggcg tggaccgatt gcgacgggca tccggagaac
     2161 gccctgagcc gggacgagat gctcgacaac atcatgctct actggctcac ggccagcggc
     2221 gcatcgtccg cacggctcta ctgggagagc ttcggcagct tctccgcagg cgacgtgagc
     2281 gtgccgacgg gatgcagcat cttcccgaaa gagatcttca cctgctcgcg ccggtgggcg
     2341 gagcagcgct tcaagaacat cgtctactgg aacgagctgg accggggcgg acacttcgcc
     2401 gccttcgagc agcctgagct gttcgtcggc gagctgagaa actgcttccg ccaggtacgc
     2461 gggtgaccga ggggcccatc tggcgcccgg atccggtccg ggcgctggaa tcgaacatgg
     2521 cccgcttcac cgaggaagcc gcccgggtat ccggccgctc catgacgggc tatgcggacc
     2581 tgcacgcctg gtcgatcgaa gagcccgccg ccttctggga tctggtctgg catttcacgg
     2641 acgtgcgagc ttcccaagcg ccaaccgcga tcctgaccaa tgcggatcag ctgccgggag
     2701 cccgatggtt cgagggcgcg aggctgaact tcgccgaaaa tctcctgcgc ttcgacgatg
     2761 atcgcccggc cctgatagcg cttttggaga ccggcgagcg gcgtgagatt tcctacgccg
     2821 agctgcgccg cgagacggca caggtcgccg cgtggctcaa aaatcacatc gacccgggag
     2881 accgggttgc cgggtggctg ccaaacgtgc cggaggcggt gaccgccatg ctggccacca
     2941 cctccctggg cggcgtctgg tcgtcgtgct cgcccgactt cggcgccgag ggcgcgctgg
     3001 atcggttcgg gcagatcgag ccgaaggtgc tgttcgcctg cgacggttac cactacaacg
     3061 gcaagcctat ctccaccgtg gacaaggcgc gccaggttgc cgagcgcatc ccctccatac
     3121 gcgccatcgt ctggatctcc ctggccgggg aagatctggt ggaaagcgac ggcgtagcct
     3181 acgcctcgct cctcgaaggc gaccacgatc tcgctttcgc ccaacgcgct tttgacgatc
     3241 ctctgtacgt catgtactcg tccggaacca ccggaaagcc caagtgcatc gtccacggga
     3301 tcggcggcac cctcatccag catctcaagg agcaccagct ccacacggat ctccgccggg
     3361 atgatcggct gttctacttc accacctgcg gctggatgat gtggaactgg ctggtcagcg
     3421 ggctcgccac cggtgccacg ctggtactct acgacggctc gccctttcat ccgggtcccg
     3481 aagccctgct ggacatggcc gagcaggaag acgtcacggt gttcggcgtc agcgcccgct
     3541 acatcgccgc ccttcagaag acttcgctca agcccatgga gactcacgat ctttcggcgc
     3601 tccgagcaat tctgtcgacc ggctcgccac tgactcacga aggcttccgc tacgtctacg
     3661 agcacatcaa gcgggacgtg ctgctcgctt cgatttccgg cggcaccgac atcatttcct
     3721 gcttcgttct gggcaacccc acgctgccgg tttgggaagg cgagattcaa tgccgggggc
     3781 tcggcatggc ggtcgaggtg gtggacgacg gcggccgacc ggtcaccggg gaaaagggcg
     3841 agctcgtctg cacccggccg tttccctcct gccccctcgg cttctggaac gacacggacg
     3901 gcgcgaagtt ccgtgccgct tacttccagc gtttccccgg catctgggct cacggcgatt
     3961 tcgccgaggt caccgagcac gacggcttcg tcattcacgg gcgcagcgac gccgtgctga
     4021 atccgggagg cgtcaggatc ggcacggccg agatctaccg tcaggtagag cacgtcgacg
     4081 cggtgctcga aagcctgtgc gtcggccagg agtgggaggg agacacccgc gtcgtgctct
     4141 tcgtcgtgat gcggcccggc cgcgagctgg acgaagcgct ggctcagcag atacgccagc
     4201 gcattcgtca gcatgcatcg ccccgtcacg ttcccgcgcg catcgtgcag gtggccgaca
     4261 ttccccgaac gctgagcggc aagatcgtag agctggcggt tcgagagacc atccacgggc
     4321 gccccgtgca gaacacgtcg gctctcgcga acccggaggc cctagatctc tatagcaacc
     4381 tgccagaact cgaaagctga ggccgcccac gcccgccagc ccgatctttg cgccccctct
     4441 tggttccgtc ccctcaaggg cccagcggct ctccttcgat ggtgatccga tgcagcaggc
     4501 gtcgcttgcc gtggtagtcg ttgacggcgt aatgccaggt tgcacggtta tcccagaggg
     4561 ccaacgaacc aggctgccaa cggaaccggt acgtgaactc agcacgcgac gcatgctggt
     4621 acaggtagag cagcagcggc ttcgactcct cctcggtcca gtcttcga
//
LOCUS       DBNHKV010000115         7243 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_512142, whole genome shotgun sequence.
ACCESSION   DBNHKV010000115 DBNHKV010000000
VERSION     DBNHKV010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7243)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7243)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7243
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_512142"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="AC16PP_03630"
     CDS             <1..661
                     /locus_tag="AC16PP_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR6480744.1"
                     /translation="VLLHQTIIGEEALLQLAKVGETPDVLVGCTGGGSNFGGLSFPFL
                     REKLAGRINPTIRCVEPTACPTLTKGQYRYDFGDTAGLTPLMKMHTLGHSFIPEPIHA
                     GGLRYHGMAPLVSHLYELGMVEAIAIPQTECFEGALQFARTEGIVPAPEPTHAIAAAI
                     REALACRESGEEKVILTALCGHGHLDLASYDKYLHGEMVDLDLSDEVIEAAMKDVPVL
                     A"
     gene            747..3161
                     /locus_tag="AC16PP_03635"
     CDS             747..3161
                     /locus_tag="AC16PP_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6480745.1"
                     /translation="MNSTRSGLGRRLSVPAAFLGLACAFSGVAQSAHSAASSVIEEIV
                     VTARMREEGLQSAPIAISAFTEETLDYRGVERLDEIAKFVPSLTLENNPSFGGASNSA
                     AIYIRGIGQKEFLPTTEPGVGLYVDGVYVARSVGAILDIIDVERLEVLRGPQGTLFGR
                     NTIGGAISITTVKPEPGGEFEAMVSGALGTDDLVHLRGSAHLPLSDTLAARLSVASFQ
                     QDGYVDRADGIDLGEDDTLTGRLAIAWEPNERFRADLSFEATRDREHGPPMELIGIDF
                     TDLSQLQGVVLAPPPPMAFIHNVTAAAAGPGQPCAVTDVNGNGITYNPSIPNCYDSRY
                     IDPESGENQGTAPAFSKTDLFGVSANLRWDLSDTLTLKSITSWRDLDSEFARDGDHSP
                     QRISQFFDDLEQQQFTQEFQLLGAHERLNWILGAYYFTEDGDNVNILDFTVSNFRSGG
                     EFDNEAWAVFAQATFDITDRLHLTAGGRYTEEEKSFLPDQIIFQNYYAGISELVPEGN
                     PLAALDAPFLQAGERILPYLEKRIDIDEFTPMVNVSFDATDDVMLYATYSEGFKSGGF
                     TQRVFPPIVPGFTAPPGTPDIDLIPTYEPEFVKSYEIGAKATGWDGRLRLNGAVFRSD
                     YKDLQVQVFNSVAPVTQNIGKASINGLELEVTLVPADDWLIEGSLSWLDAEYDEIDTG
                     ITLIGEDNEFERVPEFTASAGISKEFQLDDAGSLVVRADWSYRSETFNDAYNTPQLET
                     DGYHLIDASVRWRNAGEDWTVALSGRNLTDEKYLVSGVYGTAFQSYEGMFDRGSQWRL
                     EVRKEF"
     gene            3195..3773
                     /locus_tag="AC16PP_03640"
     CDS             3195..3773
                     /locus_tag="AC16PP_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6480746.1"
                     /translation="MARVKLAKPESVDDPAVAGIFEWVTQMEGSVPNHFYVEMNFPEF
                     FTAKLGATKVLWEAGELTMPEIQHIGILVSKDNGCRYCTAAFCTILNHGLEAAEDYVA
                     ELVSVGTDAVSDLRLRTMLEYALQVNRDAAGVTDGDVARLRNIGLSDKGIVQLTHIVS
                     DFASYNRLNLALQTDYDYRDLWRQLAFGDGAG"
     gene            complement(3897..5153)
                     /locus_tag="AC16PP_03645"
     CDS             complement(3897..5153)
                     /locus_tag="AC16PP_03645"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6480747.1"
                     /translation="MKLKTGADVDATPLEEIDVSRPELYLNDTWQPWFARLRKEAPVH
                     FLADSVNGPFWSVSSHQLIKEVDRNHQVFSSEKGGIAIVDPYVMEEGQVQGRSFIALD
                     EPKHMPQRMAVAPTVAPKNLAELEPLIRERAIDILDHLPVGETFNWVQEVSIELTARM
                     LATLFGFPYEDRRKLVYWSDLATAVPEVTGDDSIDLSKRYEELMGAAAAFYQLWAERA
                     QEPPKFDLISMLIHNEQTAKMNEDPEQFLGNILLLIVGGNDTTRNSISGGVIALNRYP
                     GEYQKLRDNPGLIPNMVSEIVRWQTPVIHMRRTALQDCELGGKQIREGDKVVMWYLSG
                     NRDETVFDDPDRLIIDRPNARAHVAFGFGVHRCMGNRLAELQLRILWEEIMKRFHTVE
                     VVGDVERLSNNFIRGIKDVPVRLHPT"
     gene            complement(5258..5695)
                     /locus_tag="AC16PP_03650"
     CDS             complement(5258..5695)
                     /locus_tag="AC16PP_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HIR6480748.1"
                     /translation="MSDEPSYGTGDATFRAAGGEAGIRRLVDAFYDIMERDPRYRRIW
                     DWHPPDNEVSRDKLARFLCAWMGGPRLFSEKYGRISIPSAHAHLGVTAVERDLWLDCM
                     ARALAQQDYPESLVAYLLKELAVPAERIRQVTEGGAKSETGDR"
     gene            complement(5783..7045)
                     /locus_tag="AC16PP_03655"
     CDS             complement(5783..7045)
                     /locus_tag="AC16PP_03655"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6480749.1"
                     /translation="MDWQQYELEHEQRLDNARAHAATLALEEIDVAHWDLMRFDTIWP
                     YFERLRREAPVYHHENSIVGPYWSVTSYELVKAVDTDARRFSSEPTIGLNVPPEQDEA
                     SSFIAMDDPKHAEQRKAVAPVAGPRNLAALAPTIRQRVADILDGLPVGEPFNWVERVS
                     IELTTQMLATLFDFPFEERYKLTRWSDIAISPRKAGLWDTLEERERELDECLSRMTEL
                     RDERIRQPGGHDLLSMLAHSESTRNMPPRQFLGNLLLLIVGGNDTTRNSISGGVLAMN
                     RWPEEFEKLKADRSKIPNAVAEIIRWQTPLAFMRRTTTEDVEIGGQTIPAGERVLMWY
                     ASGNRDENVFENADLFEVDRGNARQHLSFGFGVHRCMGNRVAELQLKILWEELLERYS
                     RIEVAGAPVRAISNFVRGYTHLPVKLHT"
BASE COUNT         1374 a         2319 c         2212 g         1338 t
ORIGIN      
        1 cgtgctgctc caccagacga tcatcggaga ggaagcactg ctgcagcttg cgaaagtcgg
       61 cgaaaccccg gacgtgctgg tgggttgcac cggcggcgga tcgaatttcg gcgggctgtc
      121 ttttccgttc ctgcgcgaga aactcgccgg ccgcatcaac cctaccattc gctgcgtgga
      181 acccactgcc tgcccgactc tgaccaaagg tcagtaccgc tacgactttg gcgacaccgc
      241 gggcctgacg ccgctgatga agatgcacac gctcggccac tctttcattc cggagccgat
      301 ccacgcaggc ggcctgcgct accatggcat ggcgccgctg gtctcgcatc tctacgagct
      361 cggcatggtg gaggcgatcg caattccaca gacggagtgt ttcgagggcg cgctgcagtt
      421 cgcccgtacc gaaggcatcg tgccggcccc cgagccgact catgccatcg cggccgccat
      481 tcgcgaggca ctcgcctgcc gggaatccgg tgaggaaaag gtcatcctga ccgccttgtg
      541 tggccacggc cacctggatc tggcgtccta tgacaagtat ttgcacggag aaatggtcga
      601 tctcgatctc tccgacgaag tcatcgaggc ggccatgaag gacgtgcccg tcctcgcatg
      661 agcgggccta gacgagcgaa cacccgcttt taaacgagca ccaccgaacg aacgacgaca
      721 gaacaagcaa tcaggggaga accatcatga actctactcg gagcggcctg ggccgccgtc
      781 tgtccgtgcc ggccgcgttc ctcggcctgg cctgtgcttt ttcgggagtc gctcagtcgg
      841 cgcacagcgc tgcgtccagc gtgatcgagg aaatcgtggt cactgcccgc atgcgtgaag
      901 aggggctgca gagcgcgccc atcgccattt cggcattcac cgaggagacg ctcgattacc
      961 ggggggtgga acgtctggac gagatcgcga aattcgtgcc cagcctgacg ctggagaaca
     1021 acccgagttt cggtggtgcc tcaaactctg ccgccatcta tatccgcggg atcggccaga
     1081 aagagtttct gcccaccacg gaacctggcg tgggtctgta cgtggacggc gtgtacgtgg
     1141 ctcgctcggt tggcgcgatc ctggacatca tcgacgtgga acgcctggaa gtcctgcgcg
     1201 gcccccaggg caccctgttt ggccggaaca caatcggcgg cgccatatcc atcaccacgg
     1261 tgaaacccga gccaggcggc gagtttgaag ccatggtatc cggcgctctg ggcaccgacg
     1321 atctggtgca cctgcgcggt tcagcccacc tgccgctgag cgacacgctg gctgccaggc
     1381 tgtcggtagc ttccttccag caggacgggt acgtggaccg tgccgacggc atcgatctgg
     1441 gcgaggacga cacgttgacc gggcgtttgg caatcgcgtg ggagcccaac gaacgcttcc
     1501 gcgctgatct gagcttcgag gccacacggg accgcgaaca cggtccgccc atggagctga
     1561 tcggcatcga cttcaccgac ctcagccagc tgcagggtgt ggttctggca ccgcctccgc
     1621 ccatggcatt cattcacaac gtcaccgccg ccgcggccgg gccgggccag ccctgcgccg
     1681 tcaccgacgt caacggcaac ggcatcacct acaacccgag catcccgaac tgctacgaca
     1741 gccgctacat cgatccggaa agcggcgaaa atcagggcac cgcgcccgcg ttctccaaga
     1801 cggacctctt cggcgtgtcc gcaaaccttc gctgggacct ctccgatacg ctgacgctga
     1861 agtccatcac gagctggcgg gatctcgact ccgagttcgc ccgggacggg gatcactcgc
     1921 cccagcgcat ctcgcagttc ttcgacgatc tcgagcagca gcagttcacc caggaattcc
     1981 agctccttgg tgctcacgaa cggctgaact ggatcctggg cgcctactac ttcaccgagg
     2041 acggcgacaa cgtgaacatc ctggatttca ccgtctccaa cttccgctcc ggcggcgagt
     2101 tcgacaacga agcgtgggcc gtcttcgccc aggcgacctt cgacatcacc gaccggctgc
     2161 acctgaccgc gggcggccgc tacaccgagg aagagaagtc cttcctgccg gatcagatca
     2221 tcttccagaa ctactatgcg ggcatcagcg agctggtacc cgagggcaac cccctggccg
     2281 cgctggatgc gcccttcctc caggcgggcg agcggatcct gccgtacctc gagaaacgca
     2341 tcgacatcga cgagttcacc cccatggtga acgtctcgtt cgatgcgacc gacgacgtca
     2401 tgctctacgc cacttactcc gaagggttca agtcgggggg cttcacccag cgcgtcttcc
     2461 cgcccatcgt tcccggattt accgcgccgc cggggacgcc cgacatcgac ctgatcccca
     2521 cgtacgaacc tgaattcgtg aagtcctacg agatcggcgc caaagccacg ggttgggacg
     2581 gccggctgcg tctgaacggc gccgtgttcc gaagcgacta caaggatctg caggtacagg
     2641 tgttcaacag cgtcgcgccc gtcacccaga acatcggcaa ggcgtccatc aatggcctgg
     2701 agctggaggt gaccctggtg ccggcggacg actggctgat agaaggcagc ctgtcgtggc
     2761 tggacgccga gtacgacgag atcgacaccg gcatcacgct gatcggcgag gacaacgagt
     2821 tcgagcgggt gccggagttc acggccagcg ccggcatctc caaggagttc cagctcgacg
     2881 atgcgggctc gctggtagtg cgcgccgact ggagctaccg ctcggaaacc ttcaacgatg
     2941 cctacaacac gccgcagctg gagacggacg gctaccacct gatcgacgcc agcgtccgct
     3001 ggcgcaacgc gggcgaggat tggaccgttg ccctgtcagg ccgcaacctg accgacgaga
     3061 agtatctcgt cagcggcgtc tacggcacgg ccttccagtc ctacgaaggg atgttcgatc
     3121 gtggcagcca gtggcggctg gaagtccgca aggagttcta gcccgattca caggggacga
     3181 cgagaggccg aaacatggca agagtgaaac tcgccaagcc tgagtccgtt gacgatccgg
     3241 cagtagccgg catcttcgag tgggtgaccc agatggaggg cagcgtgccc aaccatttct
     3301 acgtggagat gaatttcccg gagttcttca cggcgaagct cggcgccaca aaggtgctct
     3361 gggaggccgg tgagctgacc atgcccgaga tccagcacat cggcatcctg gtctcaaaag
     3421 acaacggctg ccgctactgc acggcggcat tctgcaccat cctcaaccac ggcctggaag
     3481 cggcggagga ctatgtggcc gaactggtga gcgtcggaac ggacgccgtt tcggacctgc
     3541 ggctgaggac gatgctggag tacgcactgc aggttaatcg ggatgcggcc ggcgtcaccg
     3601 acggcgacgt cgcccgcctt cggaacatcg ggctgagcga caagggcatc gtccagctga
     3661 cgcacatcgt cagcgacttc gcttcctaca accgtctgaa cctggcgttg cagaccgact
     3721 acgactatcg ggacctctgg cgccagctgg cttttggcga cggcgccggt tagcttcctg
     3781 ctgctgctgt ggcgatgatc tgcgtggatg cgatcgccaa ggtttccgcg ctcacgacgg
     3841 agggtgcctt agggctggag gaaggcaacg tcgagagcgc cgcctgaacg acgagctcaa
     3901 gttgggtgga gccttacggg gacgtccttg attcctctga tgaagttgtt ggacaaacgc
     3961 tcgacgtcgc ccaccacttc aaccgtgtga aagcgcttca tgatctcttc ccagagaatg
     4021 cgcagctgaa gctccgccag ccggttaccc atgcagcggt gcaccccgaa gccgaacgcg
     4081 acgtgggccc tggcgttcgg gcgatcgatg atcagccggt ccggatcatc gaaaacagtc
     4141 tcgtctcgat taccggagag gtaccacatc accaccttgt cgccctccct gatttgcttg
     4201 ccaccgagct cgcaatcctg cagggccgtt cggcgcatgt gaatgaccgg tgtctgccaa
     4261 cgaactattt cagaaaccat gtttgggatc aggccgggat tgtcccgcag tttctgatac
     4321 tcacccggat agcggttcag agcgatcacg cctccgctga tgctgttccg ggtggtgtcg
     4381 tttccgccca cgatcaacag cagtatgttg ccgagaaact gctcagggtc ctcgttcatc
     4441 ttcgcggtct gctcgttgtg gatgagcatg gagatcagat cgaattttgg cggttcctgg
     4501 gcccgttccg cccacaactg atagaacgcc gccgccgcgc ccatcagctc ctcgtagcgc
     4561 ttgctcagat cgatgctgtc gtctccggtc acctcgggca ccgcggtagc caggtccgac
     4621 cagtacacta gcttgcggcg gtcctcgtac gggaaaccaa acagcgtggc cagcatccgg
     4681 gccgtcagct cgatggatac ttcctgcacc cagttgaagg tttcgccaac cggcaggtgg
     4741 tcgaggatgt cgatggctct ctcacgaatc agcggctcca gctccgccag gttcttcgga
     4801 gcgaccgtgg gagcgacggc cattctctgc ggcatgtgtt tcggctcgtc cagcgcgatg
     4861 aagctgcggc cctgtacctg gccttcctcc atcacgtaag ggtcgacgat tgcgatcccg
     4921 cctttctccg acgagaacac ctggtggttt ctgtcgacct ccttgatcag ttgatgactg
     4981 gagaccgacc agaaagggcc gttcacgctg tccgcaagaa agtgcacggg cgcttccttt
     5041 ctgaggcggg cgaaccacgg ctgccaggtg tcgttcaggt aaagttccgg acgactgacg
     5101 tcgatctcct cgaggggggt ggcatcgaca tccgcacctg tcttcaactt catccgagcc
     5161 tcctgatcaa gccgtaaagt gaacgatttc agaagcatat caccgaccgg aaatccgatc
     5221 gcgaccgggc gcgcttcgag gctactgctg agagctgcta acggtctcca gtttcagatt
     5281 ttgctccgcc ctcggttacc tgtcgaatac gctcggcggg tacggcgagc tccttgagga
     5341 ggtatgcgac cagcgactcg gggtaatcct gctgcgcaag cgctcgggcc atgcagtcga
     5401 gccagagatc gcgctcgacg gcggtgacgc caaggtgcgc gtgggcggac ggaatgctga
     5461 tgcgaccgta cttctcgctg aacaaccgcg gaccgcccat ccaggcgcag agaaagcgcg
     5521 cgagcttgtc gcgtgatact tcgttgtccg gcgggtgcca gtcccagata cgccggtatc
     5581 tggggtcccg ctccatgatg tcgtaaaaag cgtctaccaa ccgtcggatg ccggcttccc
     5641 caccggcggc tctgaaggtg gcatctcccg tgccgtagct gggctcgtcg ctcacgaccg
     5701 ctgcgcccca ggagagacgt gttcgccgcc ggtaacgtcg tgaaaggtca cctcgagagg
     5761 cccattgccc gatgacccac ggtcaggtgt gcaacttcac gggcaggtga gtgtagccgc
     5821 gaacgaagtt ggagatggcc ctcaccggag cgcctgccac ttcaatacgg ctgtaacgct
     5881 ccagcagctc ctcccagagg atcttgagct gcagctcggc gacgcgattg cccatgcagc
     5941 ggtgcacacc gaagccgaag gagaggtgct ggcgagcgtt cccgcgatcg acctcaaaga
     6001 ggtcggcatt ctcgaaaacg ttctcgtccc gattcccgga cgcgtaccac atcaataccc
     6061 tttctcccgc gggaatggtc tggccaccaa tctcaacatc ctccgtcgtc gtgcgacgca
     6121 tgaacgccag cggcgtctgc cagcggatga tctcggccac ggcgttcgga atcttcgagc
     6181 ggtcggcttt gagcttctcg aactcttccg gccaccgatt catggcaagc acaccgcccg
     6241 agatggaatt tctcgtggtg tcgttgccac caacgatgag cagaagcagg ttgcccagga
     6301 actggcgcgg cggcatgttg cgggtacttt cgctgtgcgc aagcatcgag agcaggtcgt
     6361 gaccgcccgg ttggcggatc cgctcgtcgc gcagctcggt catgcgcgac aggcactcgt
     6421 cgagctcccg ctcgcgctct tccagcgtgt cccagaggcc ggctttgcgt ggagaaatcg
     6481 cgatgtccga ccagcgcgtc agcttgtagc gttcctcgaa agggaaatcg aacagggtcg
     6541 cgagcatctg cgtggtgagc tcgatggata cgcgctccac ccagttgaag ggctcaccca
     6601 caggaagacc gtcgagaatg tcagccacgc gttgcctgat cgtcggcgcc agcgcagcga
     6661 ggttacgggg acctgccaca ggcgcgaccg ccttgcgctg ctccgcgtgc ttcgggtcgt
     6721 ccatggcaat gaagctcgac gcttcgtcct gctccggcgg cacgttcagc ccgatggtcg
     6781 gctcggaaga aaaacgacgc gcgtcggtat cgacggcctt gaccagctcg tagctcgtca
     6841 cggaccagta gggacccacg atgctgtttt catgatgata gacgggcgct tcgcggcgca
     6901 ggcgctcgaa gtacggccag atcgtgtcga atcgcatgag gtcccagtga gcaacgtcaa
     6961 tctcttcgag ggccagcgtt gccgcgtggg cacgcgcatt gtccagtcgc tgctcgtgct
     7021 ccagctcgta ttgctgccag tccatcgtcg accccaatca agtccagcgc agatcatact
     7081 ccgaagcgcc cgtgctgctc gacacgccct gccaagcccc cggagagctc cgcggagggc
     7141 ccgagccggt catgaagcga gcatctccgc ccgatgcgcc ttctctacaa gactcttttc
     7201 tcgtagattg gcaaggagcg ggagtggaga agaacgatgc agg
//
LOCUS       DBNHKV010000116         3787 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_938544, whole genome shotgun sequence.
ACCESSION   DBNHKV010000116 DBNHKV010000000
VERSION     DBNHKV010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_938544"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="AC16PP_03660"
     CDS             <1..698
                     /locus_tag="AC16PP_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR6480750.1"
                     /translation="RFGHGKAEALAGLGQATFIAGSAIFLILHAVDRLMDPVTIEHVA
                     AGSAVMGFAIVMTLVLLTIQRHVIRRTGSTAIRADALHYATDLATNAATLLALLLAAY
                     GFSGLDPLFGLAIAAYILYSASRIGRDAVSLLMDRELPQAEREEIKRVVDGTPGVLGV
                     HGLRTWQSGQRKVIQMHLELDPDLPLREAHRIAVEVERRLLEHEPEADITIHQDPAGQ
                     PSHVAHNPGDAFS"
     gene            764..1225
                     /locus_tag="AC16PP_03665"
     CDS             764..1225
                     /locus_tag="AC16PP_03665"
                     /inference="COORDINATES: protein motif:HMM:NF039797.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HIR6480751.1"
                     /translation="MSTRILMLSMLLLAGCASAPEAEQAQQQREEDIATILNEPLDPS
                     EYGEAKRCLAGHEYRDFAIIDDRRIVFKGPGNSLWLNELRARCPDLRRADTLAFRSHT
                     MSERICELDSFVASDWMDSLSRRSPGMRCTLGSFQPISEPQLQALRDLSKR"
     gene            complement(1227..2099)
                     /locus_tag="AC16PP_03670"
     CDS             complement(1227..2099)
                     /locus_tag="AC16PP_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR6480752.1"
                     /translation="MSASDSQPLLIVALGGNALSPPGRGGEGYADERRIVAQTARTLR
                     DLAGLGRRLLIVHGNGPQVGRLLRTDPEPGNLDIHVAQTQGELGYLIARELAPEPVVG
                     VLTRVVVSEPLGAPVKPIGPILAERPQKAASVPAGSGWRLVVPSPKPARVVEIKAVAQ
                     LLKSSHVVAGGGGGVPLDEEGNPLNCVVDKDWVASLFAVGLHAQSLVFATDVSGVHPD
                     PERQGERIDRLSVADAEQLLASDAVAPGSMAPKLASAVEFVSSSGRSAGICDLESLAA
                     FAGGDESAGTVITP"
     gene            complement(2086..3417)
                     /locus_tag="AC16PP_03675"
     CDS             complement(2086..3417)
                     /locus_tag="AC16PP_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480753.1"
                     /translation="MSQERRRVVICGAAGRDFHNFNVAFRDDPQVQVVAFTAAQIPGI
                     DRRRYPAELAGSRYPEGIPIIPEAELESVCRQQSVSEVIFAYSDVSYAAVMEVASRVL
                     ATGAGFELLGPDATMLKAGVPVIAVCAVRTGCGKSQTARFVSSELVRGGRRVAVMRHP
                     MPYGDIAAQRVQRFASMADMDAAGCTLEEREEYEPHVAAGGVVYAGADYGAVLEQAEQ
                     GVDVLVWDGGNNDFSFIRPDFHIVVVDALRPEQLDTHYPGAAVLRTADLVVVNKVRSA
                     SADQLDQVSAGLDRIVPGVPRVLAASPVVLDEPERLTGARVLVVEDGPTITHGGMPTG
                     AGYRAVHELPVARIIDPRTCAAGSIAALYDRYPHIGPILPAMGYGPAQLDELRETVAR
                     SGADYVVAGTPIDLARALETDIPVLRARYSYVDAGSPGLMDHVKDFLDERI"
     gene            complement(3419..>3787)
                     /locus_tag="AC16PP_03680"
     CDS             complement(3419..>3787)
                     /locus_tag="AC16PP_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480754.1"
                     /translation="SLVKKHGNILFVNEPWASGASVVVLCESTPTDCERPLNVARRIA
                     DAENIELVLAVPPGAQSVERSPADRVVHLDAWSEDAVAALCDQEDARLLVLPPTEKLD
                     WRALLMNIVDRVPCSLLRLD"
BASE COUNT          659 a         1322 c         1199 g          607 t
ORIGIN      
        1 accgcttcgg gcatggcaaa gccgaggcgc tggccggtct gggtcaggcg accttcatcg
       61 ccggctccgc catattcctg atactgcacg cagtagaccg actaatggat cccgtcacga
      121 tcgagcacgt cgcggcaggc tcggcggtga tgggattcgc catcgtcatg acgctggtgc
      181 tgctgacgat tcagcggcac gtaatccgcc gcaccggttc gactgccata cgcgcggatg
      241 cgctgcacta cgcaacggac ctggccacca acgcggccac cctgctcgcg ctgttgctgg
      301 cggcctacgg tttctcaggg ttggatccgc tgttcggcct ggccatcgca gcctacatcc
      361 tttactccgc cagccgcatc ggtcgagacg ccgtcagcct gctgatggac cgggagctgc
      421 cccaagccga gcgcgaggaa atcaagcgag tcgtggacgg cacgcctggg gtgctgggcg
      481 ttcacgggct gcgcacctgg cagtccggac agcgcaaggt catccagatg cacctggagc
      541 tggacccgga cctcccgctc agagaggctc accgcatcgc cgtggaggtg gagcgacgcc
      601 tgctcgaaca cgagccggag gctgacatca ccatccatca ggatccggcg ggacagccat
      661 cccacgttgc tcacaaccct ggcgacgcgt tcagttgact catccaatgg ggtcagcggg
      721 gcgatttgtc agtcgaccgc gtttcgggct aacgttcgtc gccatgagca cccggattct
      781 aatgctctcc atgctgctgc tcgccggctg cgcgtcggcg cccgaggccg agcaggccca
      841 gcagcagcgg gaggaagaca ttgccacgat tctgaacgaa cccctcgacc cgtcggagta
      901 cggtgaggcc aaacgctgcc tggcgggcca cgagtaccgc gacttcgcca tcatcgacga
      961 ccgccgcatc gtgttcaaag gtcctggaaa cagcctgtgg ctgaacgagc ttcgggctcg
     1021 ctgccccgac ttgcggcggg ccgatacgct ggcctttcga tctcatacca tgagcgagcg
     1081 gatctgcgag ctcgacagct tcgtagcgtc ggactggatg gacagcctgt cgcgccgctc
     1141 gcccgggatg cgctgcacgc tcggttcttt ccagcccatc agcgagcctc agctgcaggc
     1201 ccttcgcgat ctgtccaagc gctgactcag ggtgtgatca ccgtgccggc ggattcgtct
     1261 ccgcccgcaa acgcggcgag gctctccaga tcacagattc ccgcggaccg gcccgatgag
     1321 ctgacgaact ccacggcgct ggcgagcttc ggagccatgg accccggcgc gacggcgtcc
     1381 gaagccagca gctgctccgc gtcggcaacg ctcagccgat cgatccgttc accctggcgt
     1441 tccgggtccg ggtgaacgcc ggacacgtcg gtggcaaaga cgagactctg ggcgtgcagg
     1501 ccaacggcaa acaggctggc aacccaatct ttgtccacca cgcagttcag cgggttaccc
     1561 tcttcgtcca ggggcacccc gccgccgccg ccggccacga cgtggctcga tttcagcagc
     1621 tgcgctacgg ccttgatctc gacgacgcga gccggtttcg gcgagggcac gactagccgc
     1681 caccccgacc ccgcaggcac cgatgccgct ttctgagggc gttcggcgag aatcgggcca
     1741 atgggcttga ccggcgcgcc gagcggctcg gagacgacca cccgtgtcag cacgcctacc
     1801 acaggctccg gcgcaagctc cctggcgatc agatatccca gctcgccctg ggtctgggcc
     1861 acgtggatgt cgaggttgcc gggctcgggg tcggtccgca gcaggcgccc gacctgaggc
     1921 ccgttcccat gaacgatcag cagtcggcga cccaggccgg ccagatcgcg cagcgtccgg
     1981 gcggtctgcg ccacgatccg gcgctcatca gcgtatcctt cgccgccccg cccgggcggc
     2041 gacagggcat tgccgcccag cgcgacgatc agcagcggct gggaatcaga tgcgctcatc
     2101 gagaaagtct ttgacgtggt ccatcaatcc gggcgatccg gcatcgacgt acgaataccg
     2161 ggcccgcaac acggggatgt ccgtctccag cgcccgggcg agatcgatgg gcgttcccgc
     2221 cacgacgtag tccgcgcctg atcgtgcgac cgtctcgcgc agctcgtcta gctgcgcggg
     2281 tccgtacccc atggccggga gaatggggcc tatgtgcgga tatctgtcat agagcgcggc
     2341 gatcgaaccc gcagcgcacg ttcgcggatc gatgatccgg gcgaccggaa gttcatgcac
     2401 cgcccgatag ccggccccgg tgggcatgcc accgtgggtg atggtcggac cgtcctccac
     2461 gacgagcacc cgggcaccgg tcagcctttc cggctcgtcc aggaccaccg gagacgccgc
     2521 gagcacccgc ggcacacccg gcacgattcg gtccagcccc gcgctcacct gatccagctg
     2581 atccgcgctc gcgctgcgga ccttgttcac caccaccaga tcggccgttc gcagcaccgc
     2641 ggcaccgggg tagtgggtgt ccagctgctc aggacgcagc gcgtcgacaa cgacgatgtg
     2701 aaaatcgggg cgaatgaacg aaaagtcgtt gttgcccccg tcccagacga gcacgtcaac
     2761 gccctgttcg gcctgctcca gaacggctcc gtagtctgcc cccgcataca cgaccccacc
     2821 ggcagccacg tggggctcgt actcttcccg ctcttccagg gtgcagccgg ccgcgtccat
     2881 gtccgccatg gaggcaaacc gctgcacccg ttgggccgcg atgtcgccgt agggcatggg
     2941 atgacgcatc acggcgacgc gcctgccgcc cctgacgagc tcactcgaca cgaagcgggc
     3001 cgtctgagat ttaccgcaac cggtgcgcac ggcacacacg gcgatgacgg gaacgcccgc
     3061 cttgagcatc gttgcgtctg gcccgagaag ttcgaacccg gcacccgtgg ccagaacccg
     3121 cgaggccact tccatgaccg cggcgtagct cacgtcgctg taggcaaaga tcacctcgct
     3181 caccgattgc tgccggcaga cggactcgag ctcagcctcc ggaatgatgg gtatgccctc
     3241 gggataccgc gaacccgcga gctcggccgg gtagcggcgc ctgtcgatgc ccggaatctg
     3301 ggcagccgtg aaggccacga cctgcacctg gggatcatcg cgaaacgcga cgttgaagtt
     3361 gtgaaaatca cgcccggcgg ctccgcagat gaccacccgg cggcgctcct ggctcacttc
     3421 agtcgagcct caacagcgag cagggcaccc ggtccacgat attcatgagc aatgcccgcc
     3481 aatcgagttt ctcggtgggc gggaggacca gcagcctggc atcctcctgg tcgcagagcg
     3541 cggcgacggc atcctccgac caggcgtcca gatgaacgac ccgatcggcc ggtgatcgct
     3601 ccacgctctg ggcgccgggc gggacggcaa gcacgagttc tatgttctcg gcatccgcaa
     3661 tccgacgggc tacgttcagc ggccgctcgc aatcggtcgg cgtggactcg cagagcacga
     3721 ccacgcttgc gccggacgcc caaggctcgt tgacgaacag gatgttgcca tgctttttaa
     3781 ccagcga
//
LOCUS       DBNHKV010000117         3509 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1079625, whole genome shotgun sequence.
ACCESSION   DBNHKV010000117 DBNHKV010000000
VERSION     DBNHKV010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1079625"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="AC16PP_03685"
     CDS             complement(<1..922)
                     /locus_tag="AC16PP_03685"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6480755.1"
                     /translation="MEATRKIRVRLWRRIAPIVVVSAFYYGVLVYPVLRILGLAWPDW
                     QPGTWQLLAIMVGPLAGRLAYEAIPCSATRWLSALALTWLGICFVAFTLVLGWEVANL
                     LLPLPGRESGWALLGLTGFACLVGYVNAQRVAVRNIDVPAPQIGKRLRVAQISDVHVG
                     SRSGRFLRRVVKRTNARDPDLVFITGDLVDFRDISQQELGSLSDLEAPAYFVIGNHER
                     YVDCEAICDRLRNLGVHVIRNDTVTIDRIQLVGIDDAEPKTQVGKILQTLEPDPHRYR
                     ILLYHRPDGARDAAAWGVNLMLCGHTHAGQI"
     gene            complement(926..2143)
                     /locus_tag="AC16PP_03690"
     CDS             complement(926..2143)
                     /locus_tag="AC16PP_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480756.1"
                     /translation="MSKAPEQPAIDYPDSDAGLDTALAAWLQDPGGADAYRQDISFRP
                     PGGAHAAPGAVEQIAAQYRFARGEHGVSVTASLDGRSLSLDAPVRLEPVSIPKPWGRE
                     LWYTGMEVRGESRVVAGEASLPLSRYLALAPARLCRRRPIVLLKILDPAATPVAGDLY
                     FEVHEHKREVYVVTHVDPTAWPDGTGAIRLGMSQSRRAAFAEDDAFRNAYLAAVKAYE
                     TVRRRIDGGERLPPETETQLREAMDGFTAFAPLRVGDVVTVHPWQPHSLQHGVRVVEF
                     QTPTYERYILSFGQRVLTQNHWDTEAVLDRIDLGPPRPESLGPVAAGIGQIADFDDFS
                     AFRVEIPPGVSRPLEIELPYAVCMGVEGRVSVNGLALDAEEACFVPASALPVTLANAA
                     ATPAVALVAAAKL"
     gene            2213..>3509
                     /locus_tag="AC16PP_03695"
     CDS             2213..>3509
                     /locus_tag="AC16PP_03695"
                     /inference="COORDINATES: protein motif:HMM:NF017809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HIR6480757.1"
                     /translation="MLPAPEICERARLARDPRFDGRFLTGVVSTGIYCRPICPARPAG
                     RDNVRYFPTAAAAEAQGYRACRRCRPEVAPGVPEWAISSDRVIRGLRLIDGGFLADQS
                     TAALAARLGVSTRHLNRLFHQELGATPSSLARSRRLHMALRLIVETDLGFCDVALQAG
                     YGSVRRFNAEVSGTFGRSPRALRGVRDEELTRELTLKLPVREPFDAPWMFRFLEQRAL
                     PGLEEVHGLEYRRRVGSGESWITVSQRGGALMLKVPAGTSASLSSILRRVRRLFDLQA
                     DSAVIDAHLARDPGLAPLVAASPGLRVPGAWDGFETAVRAILGQQVSVERARRLAETL
                     IHRCGEGEFPKPRALARFVPAAIGIPGKRWLAIAELSRAVADGVIDLDEGAARERLTD
                     RLLALPGVGPWTAGYIGMRVAKDPDAFVDGDWAVLKALQA"
BASE COUNT          559 a         1153 c         1232 g          565 t
ORIGIN      
        1 cgatctgccc cgcgtgggtg tgcccgcaga gcatgaggtt gacgccccag gccgccgcgt
       61 cccgcgcccc gtccggacgg tgatacagca gtatccggta cctgtgaggg tcgggctcga
      121 gcgtctgcag gatcttgccg acctgggtct tcggctcggc atcgtcgatg ccgacgagct
      181 gaatcctgtc gatcgtcacg gtgtcgttgc gtatcacgtg caccccgaga ttcctcaggc
      241 gatcgcagat ggcctcgcag tcgacgtagc gctcatggtt gccgatgacg aagtaggcgg
      301 gcgcttcgag gtcgctcagg gagcccagct cctgctggga gatgtccctg aagtccacca
      361 agtcgccggt gatgaaaacc aggtccggat cccgggcgtt cgtgcgtttc accacccggc
      421 ggagaaaccg gccgctgcgg gagccgacgt gcacgtcgct gatttgcgct acgcgcaggc
      481 gcttgccgat ctgtggtgcg ggaacgtcga tgttcctgac cgccacgcgc tgcgcgttga
      541 cgtagcccac caggcaggca aacccggtca gtcccagaag cgcccagccg gattcgcgac
      601 ccggcagcgg cagcagcagg ttggccacct cccagccgag caccagggtg aacgcgacga
      661 agcagatccc cagccaggtc agcgccagcg ccgacagcca gcgggtcgcg ctgcagggta
      721 tggcctcgta ggccaggcgt cccgcgaggg gacccaccat gattgcgagc agctgccatg
      781 tgcccggctg ccagtcgggc catgccaggc cgagaatgcg cagaacggga tagaccagga
      841 cgccgtagta gaaggccgaa acgacgacga tgggtgcgat gcgccgccac agcctgaccc
      901 ggatcttgcg cgtcgcctcc atggctcaga gcttggccgc cgccaccaga gcaacggccg
      961 gggtggcggc agcgtttgcc agggtcacgg ggagtgccga ggcgggcacg aagcaggcct
     1021 cttctgcatc cagggcgagc ccgttcaccg atacgcggcc ttctaccccc atgcacacgg
     1081 cgtaaggcag ctcgatttcc agcgggcggg agaccccggg agggatctcc acccgaaagg
     1141 cggagaaatc gtcgaagtcc gcgatctgcc cgatgccggc ggcgaccggc cccaaactct
     1201 ccggccgggg agggccaaga tcgatccggt cgaggacggc ctcggtgtcc cagtggttct
     1261 gggtgagcac ccgttggccg aacgacagaa tgtagcgctc gtaggttggg gtctggaact
     1321 cgacgacgcg cacaccatgc tgcagcgaat gcggctgcca ggggtggacg gtcaccacgt
     1381 caccgacccg cagcggcgca aaggccgtga agccgtccat ggcttcgcgc agctgcgtct
     1441 cggtttccgg cggcaaccgt tcgcccccgt caatgcggcg gcgaacggtc tcgtaggctt
     1501 tcaccgccgc gaggtaggcg tttcggaacg cgtcgtcctc ggcaaaagct gcgcgtcgcg
     1561 actggctcat cccgagccgg atcgcccccg tgccgtcagg ccatgcggtc gggtccacgt
     1621 gggtgacgac gtacacctcg cgcttgtgct cgtgcacctc gaaatagagg tcgcccgcca
     1681 cgggcgtggc ggcaggatcc agaattttca aaaggacgat cggacgacgc cggcacaggc
     1741 gcgccggtgc gagcgcaaga tagcgcgaca ggggcaggct ggcctcgccg gcaaccacgc
     1801 gactttcgcc gcgtacctcc atacccgtgt accacagttc ccggccccag ggtttcggta
     1861 tgctgaccgg ctccaggcgg acgggggcat cgagggacag gcttcgtcca tccagcgacg
     1921 cggtcacgct cacgccgtgc tcgcctcggg cgaatcggta ttgcgcggca atctgctcga
     1981 cagcgcccgg agcagcgtga gcgcccccag gcggtcggaa ggaaatgtcc tgacgatagg
     2041 cgtccgcccc accgggatcc tgcagccaag cggccagggc cgtgtccagc ccggcgtcgc
     2101 tgtcgggata gtcaatggcg ggctgttcgg gcgctttgga caaatgtccg attccggcga
     2161 gttttttcgc gaggccggga ttatagtcgg ggaagctgct gaagaggcca gcatgttacc
     2221 tgccccagag atctgcgagc gcgcccgcct ggccagagac ccccgcttcg acggccggtt
     2281 cctcaccggc gtcgtcagca ccgggatcta ttgcaggccc atctgcccgg cccggcccgc
     2341 cggccgggac aacgtgcgct actttcctac ggctgcggcg gcggaagccc aagggtatcg
     2401 ggcatgtcgc cgctgtcggc cggaggtcgc gccgggggtg ccggagtggg cgatctccag
     2461 cgacagggtg atcagggggc tgcggctgat tgacggggga ttcctggccg atcagagcac
     2521 ggccgcgctc gcagccaggc tcggagtgag cacccggcac ctcaacaggc tctttcacca
     2581 ggagctcggg gccacgccca gcagcctggc gaggtcccgg cggctgcaca tggctctccg
     2641 gctcatcgtc gagaccgacc tgggcttctg cgatgttgcc ctgcaggcgg gctacggcag
     2701 cgtccggcgc ttcaacgccg aggtgagcgg gacgttcgga cgttcgcctc gggctctgcg
     2761 cggggtgcgt gacgaagaac tgacccggga gctgaccctc aagctgccgg tccgtgagcc
     2821 tttcgatgcg ccgtggatgt tccgctttct cgagcaacgc gcgttgcccg gtctcgagga
     2881 ggtgcacggg ctcgagtatc gccggcgggt cggatccggg gaaagctgga tcacggtatc
     2941 ccagcgcggg ggcgcgttga tgctgaaagt gcccgcgggc acttcggcgt cgctgtcctc
     3001 gatacttcgg cgcgtcaggc ggctgttcga cctccaggcg gactctgccg tcatcgacgc
     3061 gcacctcgcg cgggatccgg gccttgcgcc cctcgttgcc gcgtcgccgg gattgagggt
     3121 gccgggtgcc tgggacggct tcgaaaccgc cgttcgcgcc atactgggac aacaggtcag
     3181 cgtggaacgg gccagacgcc ttgccgagac cctgatccac cgctgcgggg aaggggagtt
     3241 cccgaagccc cgcgccctgg cgcgcttcgt gccggccgcc attggaattc cgggcaagcg
     3301 ttggcttgcc attgccgagc tttcccgggc ggtggcggat ggtgtcatcg atctcgacga
     3361 gggtgcagct cgtgaacggc tgacggaccg actgctggct ctacccgggg tgggtccgtg
     3421 gacggccggc tacatcggca tgcgggtcgc gaaggatccc gatgcgttcg ttgacgggga
     3481 ctgggcggtg ctcaaggcgt tgcaggcca
//
LOCUS       DBNHKV010000118         6266 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1505384, whole genome shotgun sequence.
ACCESSION   DBNHKV010000118 DBNHKV010000000
VERSION     DBNHKV010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6266)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6266)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6266
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1505384"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..520)
                     /gene="rnpA"
                     /locus_tag="AC16PP_03700"
     CDS             complement(98..520)
                     /gene="rnpA"
                     /locus_tag="AC16PP_03700"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR6480758.1"
                     /translation="MAARSSTRGAPKVANASPSEAGDRAAASIHSFPRRRRLTRSRDF
                     DRVLKNAEVRRRSGPLRLTAVANTMQGARLGMIVGKRAVGRAHERNRMKRVIRDEFRR
                     RHAELADMDMVILVMAELENRELRAHLARLFESEVQKR"
     gene            complement(462..596)
                     /gene="rpmH"
                     /locus_tag="AC16PP_03705"
     CDS             complement(462..596)
                     /gene="rpmH"
                     /locus_tag="AC16PP_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR6480759.1"
                     /translation="MKRTFQPSNLKRKRTHGFRSRMSTRGGRQVINARRAKGRKRLSV
                     "
     gene            1151..2476
                     /gene="dnaA"
                     /locus_tag="AC16PP_03710"
     CDS             1151..2476
                     /gene="dnaA"
                     /locus_tag="AC16PP_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR6480760.1"
                     /translation="MGRSVWHKCLDRLQDELSAQQFNTWIRPLQARQHDSALVLMAPN
                     RYVKNQVQVAYLDRITELLDQLGDPLSPLEVKVDVGIADEGESRPLRADGSGGARIQP
                     VHQLNGEFRFETFVEGKSNELAKAAALQVAANAGKSYNPLLLYGGVGLGKTHLMQAVG
                     NCVLDENPDAKVVYLHSQRFVQDMVKALQQGTMQDFMQYYRSVDTLLIDDIQFFAKKL
                     RSQEEFFHVFNGLLERGHQMVLTCDRYPREIDGLEERLQSRFVWGLTVEVEPPELETR
                     VAILMKKAEAEQVDLDPAVAFFIGERIRSNVRELEGALRRVIANARFTGTRITIEQVK
                     RALRDLLAIQDRQVSIDNIQRTVAEYYNIKISDILSKRRNRSIARPRQMAMCLAKELT
                     NHSLPEIGEAFGGRDHTTVLHACRKIAELRESSADVAEDFKNLNRVLSS"
     gene            2496..3599
                     /gene="dnaN"
                     /locus_tag="AC16PP_03715"
     CDS             2496..3599
                     /gene="dnaN"
                     /locus_tag="AC16PP_03715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR6480761.1"
                     /translation="MKFTTNREALLRPLQLVTGVVERRQTLPVLANLLVRARDGVLSM
                     TGTDLEVELVAESSDLEIERDGEATIPARKLADIWRSLPEGAEVTVSVEGDRANVRSG
                     RSRFSLATLPAADFPKVESGPGDLELKLDQSSLRSLLDQVSFSMAQQDVRYFLNGMLL
                     EVTTEHTRSVATDGHRLAMCTLPGGAAGVDRVQAIVPRKGVLELSRLIDDGDDEVVVQ
                     MGANHLRVTQGPYSLTTKLVDGKFPDYEKVIPRDAARSVTGDRETLKQAFTRASILSN
                     EKYRGVRLIVEPEQLTIQANNPEQEEAEEIVPVDYEGERLEIGFNVSYLQDVLNVLDT
                     DEIKISVSDANSSALVEGTGNENAVFVVMPMRL"
     gene            3699..4793
                     /gene="recF"
                     /locus_tag="AC16PP_03720"
     CDS             3699..4793
                     /gene="recF"
                     /locus_tag="AC16PP_03720"
                     /inference="COORDINATES: protein motif:HMM:NF014516.7"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR6480762.1"
                     /translation="MHFERLEISHFRILDDVALDLNPGLNFFHGDNGAGKTAILEAAH
                     LLVRGRSFRSHRPRELIQQGQEQLTVRARVADGAMGQRTIAMGRDLSGRTELKINGMQ
                     ERRLSEAARLIPLQIMLPNVADLVLGGPGVRRQWLDWGVFHVKPDYLRTQREYVRLLR
                     QRNAALKAAYVSSPEFEAWTAQLVQAADRVDAQRKSHIAEVGPLFRATLARLAPDLEV
                     ELIYRRGWPSGETLDKVLGEWAPREVKLGSTQAGPHRADVELRVGDARAAGLLSRGQA
                     KAVASAMMVSQAQLLADGLKQTSVFLIDDVGAELDEGHNARFFQLLDDMDSQILATST
                     RLPLGGSCFAGERMTVFHVEHGRAQRSLND"
     gene            4786..>6266
                     /locus_tag="AC16PP_03725"
     CDS             4786..>6266
                     /locus_tag="AC16PP_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HIR6480763.1"
                     /translation="MTDQSYDSRNIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVQEL
                     VDNAIDEALAGHCDVIDVVVHPGEAVTVRDNGRGIPVDVHPEEGVSAAEVIMTTLHAG
                     GKFDDNSYKVSGGLHGVGVSVVNALSEEFRLTVRRDGRVYEQIYNDGVPAAPLKEVGE
                     TERRGTECFFKPSDKTFSNIEFHYEVLAKRLRELAFLNSGVHINLQDERTGKQESFVY
                     EGGLRAFVEYLNQKKNPLNEVFHFSVEREDGIGVEVAMQWNDSFQEQVFAYTNNIPQG
                     DGGTHLAGFRGALTRSLNNYIEREGLLKKIQVSTTGDDAREGLTAVVSVKVPDPKFSS
                     QTKEKLVSSEVKPAVEQELARYFTEYLDENPQDARAVVSKMIDAARAREAARKAREMT
                     RRKGALDIAGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRRTQAILPLRGKI
                     LNVEKARFDKMLSSVEVGTLVTALGCGIGRGEFDPEKLRYHRV"
BASE COUNT         1281 a         1774 c         1977 g         1234 t
ORIGIN      
        1 gggtagaagc ggcactggcg ccccaggtac ggactcaacg tccactgata cacacggatg
       61 cccgcctgaa gcaagcggcg cggtgcgttg cgaagcatca tcttttttgc acctccgact
      121 caaacagcct cgcaagatgg gcgcgaagct ctcggttctc cagctcagcc atcaccagaa
      181 tgaccatgtc catgtcggcc agctctgcgt ggcgtcgacg aaactcgtcc ctgatcacgc
      241 gcttcattcg gttgcgttcg tgggccctgc cgacggcccg cttgccgact atcatcccca
      301 ggcgggcgcc ctgcattgta ttcgctaccg ccgtcagccg taacggcccg ctccggcggc
      361 gcacctcggc gttcttcagg acgcggtcga agtcccgcga tctggttagc cggcgccttc
      421 gcggaaatga gtggatcgaa gcagctgcgc gatcgcctgc ctcagacgga gaggcgtttg
      481 cgacctttgg cgcgcctcgc gttgatgacc tggcggccac ctcgagtgga catacgcgaa
      541 cggaagccgt gagtgcgctt gcgctttaga ttgcttggct gaaacgttct cttcatgttc
      601 gggtttccgt tgctgactta ccgaagctcc cgcccctttg gcgcgcttta gtcggcggcc
      661 tggatcgcga gggagcggga ttttatggat caggcccctt gagtgtcaac gcagctgcga
      721 catcactcct tctcatagta agaaatatat atagataatt tgtagttgtt gcttgttgag
      781 acggcagcat ctgttgatat cgggcgtaag tctttgtccc ggcaagttgt tttcctcgct
      841 gcaagggtgt ggatatgctt ggggcccgtt gaggacccgg gcgggatcat cgggggtaaa
      901 gcgagcggcg gaaaactatc cacaggttct tcaacgagtt gctcacatcg ccgcaagctc
      961 gaatcgcgac ctcaccatgg ccaacggcag acgtaagttt ctgaatttgc agtcgatacc
     1021 aaaaaacctg ccggaaatgc ttcctattgc atggcaaatg ctttcgttta ctggctagaa
     1081 tgcttcgcag ttcgcgcaca tcaaaactac agattctctg ccaagagaac gggtcatacg
     1141 ggggcatttg gtgggacggt ccgtttggca taagtgcctt gatcggttgc aggacgagct
     1201 ttccgctcag cagttcaaca cttggataag acctctgcag gcacgtcagc acgacagcgc
     1261 cctggttctc atggcgccca atcgctatgt gaagaaccag gtgcaggtcg cctatcttga
     1321 tcgaataacc gagctgctcg atcagctggg agatccgttg agcccgctcg aggtcaaggt
     1381 ggacgtgggc atagccgatg agggcgagtc ccgaccgctt cgtgccgacg gcagcggcgg
     1441 cgctcgcatt cagccggttc atcagttaaa cggcgagttt cgtttcgaga cgttcgtcga
     1501 gggcaagtca aacgagctgg ccaaggccgc cgcccttcag gttgcggcca acgccggcaa
     1561 gtcgtacaac ccgttgctgc tctatggcgg ggtgggcttg ggcaagacgc atctcatgca
     1621 ggcggtcggc aactgtgtgc tggatgaaaa tcccgatgcc aaggtggtct atcttcactc
     1681 tcagcgcttc gttcaggaca tggtcaaagc gctccagcag ggcaccatgc aggacttcat
     1741 gcagtactac cgttcggtgg ataccctgct catcgacgac attcagttct ttgcgaagaa
     1801 gctgcgttcc caggaggaat tctttcacgt tttcaacggc ctgctcgagc gtggccatca
     1861 gatggttctc acctgcgacc gctatccgcg ggagatagat ggtctagagg agcggctgca
     1921 atcgcggttc gtttggggtc tgaccgtcga ggtggagccg cccgagctcg aaacccgggt
     1981 agccattctg atgaagaagg cagaagcgga gcaggtcgat ctggacccgg cggtggcgtt
     2041 cttcatcggc gagcggatca ggtcaaacgt gcgggagctc gaaggagcgt tgcgcagagt
     2101 gatagccaat gcgcgtttca ccggaaccag gatcaccatc gagcaggtca agcgggcgtt
     2161 gcgggatctg ctcgcgattc aggatcggca ggtcagcatc gacaacattc agcgcaccgt
     2221 cgctgagtat tacaacatca agatttcgga tattctgtcc aagcggcgca accggtccat
     2281 agcccgaccc cggcagatgg caatgtgtct tgccaaggag ctgaccaacc attctctgcc
     2341 ggagatcggt gaagcgttcg gcggcaggga tcatacgacg gttcttcacg cctgccgcaa
     2401 gatcgcggag cttcgggaaa gtagcgccga cgtggctgag gacttcaaga acctcaaccg
     2461 cgtgttgtcc agctgatttc acatcgtagg aagacatgaa attcacgacc aatcgggaag
     2521 ccctgcttcg accgctgcag ttggtgacgg gggtcgttga acggcgccag accctgcccg
     2581 tgttggcaaa cttgttagtc cgcgcccggg acggcgtgct gtcgatgacc ggcacggacc
     2641 tcgaggtcga gctggtagcg gagtcgtcgg atcttgagat cgagcgggat ggagaggcta
     2701 ccatccccgc ccggaagctg gcggacatat ggcgatcgct ccccgagggc gccgaggtta
     2761 cggtttcggt ggaaggcgac cgcgccaacg tgcgctccgg ccgcagccgt ttttctctag
     2821 ccaccctgcc agcagcagat tttcccaaag tggaaagcgg ccctggcgac ctggaactga
     2881 agctcgatca gtccagtctt cgaagcctgc tagatcaggt gagtttctcc atggcccagc
     2941 aggacgtacg ctactttctt aacggaatgt tgctggaggt caccacggaa cacacacgct
     3001 ccgtggcaac ggacggtcac aggctggcca tgtgcacgct gcccggcgga gcggcgggtg
     3061 tggatcgggt gcaggccatc gttccccgaa agggtgtgct cgaactcagc cgcctcatcg
     3121 acgatgggga tgacgaggtc gtggtccaga tgggcgcgaa tcacctgcgg gtcacccagg
     3181 ggccctactc tctgacgacc aagctggtgg acggcaagtt tccggactac gagaaggtca
     3241 ttcctcgaga tgcggcccgg tcggtcaccg gcgatcgcga gacgctcaaa caggcattca
     3301 cgcgtgcttc cattctttcc aacgagaagt atcgtggcgt gcgcctgatc gtcgagccgg
     3361 aacagctgac cattcaggcg aataacccgg agcaggaaga ggcggaggag atcgttccgg
     3421 tggattacga gggagagcgc ctggagatcg gtttcaacgt cagctacctc caggacgtgc
     3481 taaacgtgct ggatacggac gagatcaaga tcagcgtatc cgacgcgaac agcagcgctc
     3541 tcgttgaagg caccggcaac gagaacgcgg tcttcgtcgt catgcccatg cggctctagc
     3601 cccaaggcgc gggtgacccg cgcctgcacg ccgtggacag gggacgcctg cgctgcaaaa
     3661 gcggataatg caatgatgtt cgtcgaaccc gcccgaacgt gcatttcgaa cggttagaaa
     3721 tttcccattt tcggatactg gatgatgtgg ctctggatct gaatccgggt ctcaacttct
     3781 ttcacggaga taacggcgcg ggcaaaacgg ccattctcga ggccgcgcat ctcctggtcc
     3841 gcgggcggtc ttttcgaagc caccgcccca gggagctgat tcagcagggc caagagcagc
     3901 tcaccgtcag ggcgagggtg gcggatggag ccatgggcca gcgcaccatc gccatggggc
     3961 gggacctgtc cgggcgtacc gagctgaaga tcaacgggat gcaggaacgc cggctctccg
     4021 aggccgcgcg gctcataccg ctgcagatca tgttgccaaa cgtggcagat ctcgtgcttg
     4081 gcggccctgg agttcgccgc cagtggctgg actggggcgt gtttcacgtg aaacctgatt
     4141 acctcaggac gcagcgggag tacgttcgac tacttcggca acgtaacgcg gcgttgaagg
     4201 ccgcctacgt ttcctcgccg gagttcgagg cctggaccgc gcagctcgtc caggcggcag
     4261 accgggtaga cgcccagcgg aaaagccaca ttgcagaggt tggcccgctg tttcgtgcca
     4321 ctcttgcccg acttgccccg gacttggagg tcgagctcat ctatcgcaga ggttggccct
     4381 ccggagaaac cttggacaaa gtgttgggcg aatgggcgcc gagagaggta aaattgggtt
     4441 ctacgcaggc agggccccat cgggcggacg tggagttgcg ggtaggtgat gctcgggccg
     4501 cggggctgct gtcccggggt caggcaaaag cggtcgccag tgccatgatg gtcagccagg
     4561 ctcagctgct ggccgatggg ctcaagcaga ccagcgtatt tctgatagat gacgtgggcg
     4621 ccgagttgga cgaaggtcat aacgcccgtt tttttcaatt gctcgacgac atggatagcc
     4681 agattctggc tacgtccacc cgtctgcctc taggtggcag ttgcttcgcc ggagagagga
     4741 tgacggtgtt tcacgtggaa cacggtcgcg cgcaaaggag cctgaatgac tgatcaatca
     4801 tacgattcac gcaacatcaa ggtgctcaaa ggccttgacg ccgtccgcaa gcgcccggga
     4861 atgtacatcg gtgataccga tgacggcacc ggcctgcatc acatggttca ggagttggtg
     4921 gacaacgcca tcgatgaagc cctggccggc cattgcgacg tcatcgacgt ggtggtccac
     4981 ccaggagaag cggtaacggt gcgcgacaac ggtcggggca tacccgtgga cgtccacccg
     5041 gaagaaggcg tgtccgcagc cgaggtaatc atgaccacgc tgcacgccgg cggcaaattc
     5101 gatgacaata gctacaaggt ttctggcggc ctgcacgggg tcggcgtttc ggtcgtcaac
     5161 gcgctttcag aggaattccg cctcaccgtt cgccgcgatg ggcgcgttta cgagcagatc
     5221 tacaacgacg gggtgcctgc ggcaccgttg aaagaggtcg gcgagacgga gcgtcgcggt
     5281 acggagtgct tcttcaagcc ctctgataag accttcagca acatagagtt tcactacgag
     5341 gtattggcca agcggctgcg ggaactcgcc ttcctcaact cgggcgttca catcaacctt
     5401 caggacgagc gcacgggaaa acaggagtct ttcgtctacg aggggggcct gcgggctttc
     5461 gtggagtacc tcaaccagaa aaagaacccg ctcaatgagg tctttcactt ctcggtcgaa
     5521 cgggaagacg gcatcggcgt agaagtagcc atgcagtgga acgacagctt tcaggagcag
     5581 gtgttcgcgt acaccaacaa catcccgcaa ggtgatggcg gcacccatct ggccggattc
     5641 cgcggcgcgc ttacccgatc gttgaacaat tacatcgagc gggaggggct gctgaaaaag
     5701 attcaggtct ccaccacggg ggacgacgcc cgtgaaggtc tgacggctgt agtgtcggtg
     5761 aaggtgccgg atccaaagtt ttcttcacag accaaggaga agctggtttc cagcgaggtc
     5821 aaaccggccg tggagcagga gcttgcccga tactttacgg agtatctcga cgagaacccc
     5881 caggatgcca gggccgtggt gtcgaagatg atcgacgcgg cgcgagcccg ggaagccgcg
     5941 cgcaaggcca gagaaatgac tcgccgcaag ggcgccctgg acatcgccgg gctgccgggc
     6001 aagctggcgg attgtcaaga aaaggacccc gcgctgtcag agctttatct ggtggagggc
     6061 gactccgccg gcggctccgc caaacagggg cgcgatcggc gcactcaggc catccttccc
     6121 ctgcgtggaa aaattctcaa tgtggagaag gcgcgttttg acaagatgct ttcctctgtg
     6181 gaggtcggca cgctggtcac tgcccttgga tgtggaatcg gacgtgggga attcgatccg
     6241 gagaagctta ggtaccatcg ggtcat
//
LOCUS       DBNHKV010000119         6667 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1362487, whole genome shotgun sequence.
ACCESSION   DBNHKV010000119 DBNHKV010000000
VERSION     DBNHKV010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1362487"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /gene="yjgA"
                     /locus_tag="AC16PP_03730"
     CDS             <1..309
                     /gene="yjgA"
                     /locus_tag="AC16PP_03730"
                     /inference="COORDINATES: protein motif:HMM:NF003593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HIR6480764.1"
                     /translation="RQLQRIGKLMREIDTAPIEAALERLEGRSASARYEQHQLERWRD
                     RLIEDPAALTDYLAEHPHSERQTLRHLIERARTAGNEQQQKTASRALFRFLREDVPTG
                     "
     gene            complement(356..1798)
                     /gene="tldD"
                     /locus_tag="AC16PP_03735"
     CDS             complement(356..1798)
                     /gene="tldD"
                     /locus_tag="AC16PP_03735"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017130658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HIR6480765.1"
                     /translation="MTDLLEEAQGQLWAPAGLEQSDLDRLMGRLSSRSIDVGELFFQH
                     QRSESWALEDGIVKDGSFSVDRGVGVRAISGEKTGFAYAEDIILPALTSATEAARSIA
                     RHGEDRSVPAWQVPRVPARYPSVDPISTLDGSEKVALLRRVDEAARQADPRVKEVNVR
                     LSASHESILVMASDGTLCADVRPLVRLDVSVIVEQDGRRERGSAGAGGRGGLGLVSDS
                     EFTDGLAREAVRTALVNLEAVDAPAGPMPVVLGHGWPGVLLHEAVGHGLEGDFNRKGS
                     SAFANRVGEKVASELCTVVDDGTLDGRRGSLTVDDEGSPSQETVLIENGVLRGYMQDK
                     LNARLMKTSSTGNGRRQSFAHLPMPRMTNTFMKAGEHDPEEIIASVDRGIYAVNFGGG
                     QVDITSGRFVFSATEAYLIEKGRVTHPVRGATLIGNGPEVMTRISMVGNDLELDRGIG
                     VCGKDGQSVPVGVGQPTLKIDEVTVGGTSA"
     gene            complement(1808..5587)
                     /locus_tag="AC16PP_03740"
     CDS             complement(1808..5587)
                     /locus_tag="AC16PP_03740"
                     /inference="COORDINATES: protein motif:HMM:NF024516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family phospholipid transporter"
                     /protein_id="HIR6480766.1"
                     /translation="MHRIVLRPFLVVVTLTMLFVALMQGTGRLGFMFLPDLELVANQW
                     LSGQRVRVSGLEGSWRYLNPVVRAARVDLPAGHLREVVVELDWLESLVRNRFVARRVL
                     VGEGAVRLEKTPAGKWRLAGTSGDGEFDASGIVYHADELRFSGVIAFGLAGEEEAQSV
                     QVDYLATNRGGLHRHRLHLDNQDADCAADCGLHLEYQARDGRWPFWDERIQLSLDGGG
                     FVIPAAVLDSPLELMSIGIDWHLRGDESGGRLHAHARGLRLPGGVELAGLVRADVRGF
                     EDVHDAVVHDLAFGLVAHLDDSEEQVRTQRALRMPAVHVRKDAEVVQFWAQRVDMGGV
                     SEFLERALRGSERARRWLSALKLQAEALNVRGYYRLPTGESGFAATLAGLDVDGYRGA
                     PFVRGAAGELLGHARGLELKLKSEDVLLKFPQTFTDTWQMPYLQGAFQAWFGNGYLGL
                     RGVNLRAETLFSRAAGSFALTRPPGERYEERLSLLIGVDALDTDQAFTFVPYTIDEGL
                     EDWLRGGLKAGLLTRIRFGYHGQQHLKVNELARRIELSARISDGRIAYAPDWPDVHEL
                     DADVVVAGPMVRVTVDKGASEGVRLGGSQVVLRNNASYADVELVSRMETDLALDFVRT
                     TPLAESMGFVAPEWVGDGRLEVLGNLHIPIKLPEDVDASEQLQVDVNVRMLDAGLRMP
                     DFRLALGDLEGDLRYRYPNGLRSSRISGTLFGNDVLIDADSNSQEMILSFAGRATHED
                     VLAVLDMEDPGVVTGSADFLASLHVGVDPDHVTRLEVVSDLQGMALDLPADLRKPAEV
                     ARPVEVAVQFLDERQIISFRHGTAQGWLHVADAPERGAVGFGQPPPVLDGRADYLLLT
                     GDLEGFALEEVVPDEEVGGSGFMPLKLSGLRVGQIRLDEFVIFDAVLDGDITAEGFAI
                     TIDSEAVVGRVSMDGDAPLVLGLDRVVLPAGDGEGDPLSPQMAPELPEAVVEVGEFRV
                     GEADYGRWKFNVHPEPDGVRLADLEAVVRGVTVSSPEGVYWSTVDGESRFKGELAAEN
                     LFDVLPQWGFAPSMETESAGMTGELSWAGSPANVDLDLLVGTASVRAETGSFVDVESG
                     GGAVRMFSLINFDTIAKRMRGDFSDLTGKGLSFDKLKATVSFDRGHLAFVEPLKVKGS
                     GSEFEVAGNVDLVTGELDNEMIVTLPVSKSLPWYAAYIALANPLAGAGILVGERVLRK
                     PLEQFSSAKYEIGGTLDEPQVNLVSVFESEMVEAELESLAGAGDEDTFTELEPQE"
     gene            complement(5726..>6667)
                     /locus_tag="AC16PP_03745"
     CDS             complement(5726..>6667)
                     /locus_tag="AC16PP_03745"
                     /inference="COORDINATES: protein motif:HMM:NF021653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HIR6480767.1"
                     /translation="DVRVLLRIWDRIMARRAECGCPGIVYEELPLHTRVIRDMATAEL
                     ECIRIDHPPTLERVRKFVAEFLPELTDKVVLYDEPRPIFERFGVDDELTRALGSRVEL
                     KSGGHLVIEQTEAMTTVDVNTGAYLGKHNLEETVFRTNLEAAQVLPRQLRLRNLGGII
                     VVDFIDMEDEEHRRQVLRALEKACEGDPARIRIEGFSSLGLVQMSRKRTRESLVQQLC
                     EPCSHCHGAGVVKTAQSTCIEIFRSIMQDAESGCGAHSQGDYVIRAADNVVDRLLDED
                     ADRLAAMAARVGRRVRIELEPSYGAGEFDIVLVGDTQ"
BASE COUNT         1175 a         2370 c         2029 g         1093 t
ORIGIN      
        1 cgccagctcc agcgcatcgg caagctcatg cgcgagatcg acaccgcccc gatcgaggcg
       61 gccctggagc gtctcgaggg gcgctcggcc agcgccaggt acgagcagca ccagctggaa
      121 cggtggcgcg accggctgat cgaggatccc gccgcgctca ccgactacct cgccgaacat
      181 ccgcactccg agcgccagac gctgcggcac ctgatcgagc gggcccgcac ggcaggcaac
      241 gagcagcagc agaagacggc atcccgagcc ctgttccgct ttctgcggga agacgtgccc
      301 accggatagg cacgctcggc gggcgacgcg cgcgcggccc caatcgccat tgcgttcacg
      361 cgctcgttcc gccgacggtg acctcgtcga tcttcaacgt gggctggccc acgcctaccg
      421 gaaccgactg cccgtccttg ccgcaaacgc cgatgccgcg atcgagctcg aggtcgttac
      481 caaccatgga tatcctggtc atgacctcgg gcccgttgcc gatgagggtg gcaccacgga
      541 caggatgggt cacgcgaccc ttctcgatga gataggcctc ggtggctgag aaaacgaacc
      601 tgcccgaggt gatgtccacc tgaccgccgc caaagttgac ggcatagatg ccgcggtcga
      661 cgctcgcgat gatctcctcg ggatcgtgct ccccggcctt catgaaggta ttggtcatcc
      721 gcggcatggg caggtgcgca aaagactggc ggcgcccgtt acccgtgctc gatgtcttca
      781 tcagcctggc attgagcttg tcctgcatgt agccccgcag cacgccgttc tcgatgagca
      841 ccgtttcctg gctgggcgag ccctcgtcgt ctacggtcag cgacccacgg cgcccgtcga
      901 gggttccgtc gtccaccacc gtgcacagct cgctcgcgac tttctcgcca acgcggttgg
      961 caaacgccga gctgcccttg cggttgaagt ccccttccag accgtgcccc accgcctcgt
     1021 gcagcagcac cccgggccag ccgtggccga gcacgaccgg catgggtcct gccggcgcgt
     1081 cgacggcttc cagattcacc agcgccgtac gcaccgcctc ccgggccagg ccgtccgtga
     1141 attcagaatc gctgaccagg ccgaggcccc cgcggcctcc ggctccggcg gagccccgct
     1201 cccgacggcc gtcctgctcg acgatcaccg agacgtccaa gcgcaccagc ggcctgacat
     1261 cggcacagag cgtgccgtcg gaggccatca ccaggatcga ctcatggctc gccgagagac
     1321 gaacattcac ctctttgacg cgcgggtctg cctgccgggc cgcctcgtcg acgcgccgca
     1381 gcagcgcgac cttctcggag ccatccagcg tcgagatggg atcgacgctg ggatagcggg
     1441 cgggcacgcg cggtacctgc caggcgggca cgctccggtc ttctccgtgg cgggcaatgg
     1501 atcgcgcagc ttccgttgcg ctcgtcagcg cgggcagaat gatgtcttcg gcgtaagcaa
     1561 aacccgtctt ctcgccgctg atggcgcgca cgcccacgcc acggtcgacg ctgaaactcc
     1621 cgtctttgac gataccgtct tccagcgccc aggactcgga gcgctgatgc tgaaagaaca
     1681 gctcgcccac atcgatggag cggctgctga gccggcccat caacctgtcg aggtctgact
     1741 gttccaggcc cgccggcgcc cagagctggc cttgagcctc ttcgagcaga tcagtcacga
     1801 cgccgcatta ctcctgtggt tccagttccg tgaaagtgtc ctcgtcgccg gcgcctgcca
     1861 gcgactccag ttcggcctcg accatctcgc tttcgaaaac gctgacgaga ttgacctggg
     1921 gctcgtccag cgtaccaccg atctcgtact tggcactgct gaactgctcc aggggtttgc
     1981 gcagcaccct ttcgccaacc agtatgcccg ctccggccag cgggttggcg agagcgatgt
     2041 aggccgcgta ccacggcagg ctcttgctga ccggaagcgt cacgatcatc tcgttgtcca
     2101 gctctccggt gacgagatcc acgttccccg caacctcgaa ctcggatcca gagcccttca
     2161 ccttgagggg ctcgacgaat gccaggtggc cgcgatcgaa actgacggtg gccttgagct
     2221 tgtcaaagct caggcccttg ccggtgaggt ccgagaagtc gccgcgcatg cgcttggcga
     2281 tggtgtcgaa gttgatgagg ctgaacatgc gcaccgcgcc gccccccgac tcgacgtcga
     2341 cgaagctgcc ggtctcggcc cgaaccgagg cggttccgac cagcaggtcg agatccacgt
     2401 tggccggcga gccggcccag ctgagctcgc ccgtcatccc ggcggattcg gtttccatgc
     2461 tgggcgcaaa gccccactgg ggcagcacgt cgaagaggtt ctcggcagca agctcgccct
     2521 tgaaccggga ctcgccgtcg accgtggacc agtacacgcc ctccggagag gacaccgtga
     2581 cgccccgcac gacggcctcg agatcggcaa gccggacgcc gtccggttcg gggtgaacgt
     2641 tgaatttcca gcggccgtaa tccgcttcgc ccacccggaa ctcgccgact tcgaccaccg
     2701 cctccggaag ctccggggcc atctgcgggc tgagcggatc cccctcgccg tcaccggcgg
     2761 gcaggaccac ccggtctaag ccaagcacga gcggcgcgtc gccgtccatc gacacgcgac
     2821 cgaccacggc ttcgctgtcg atcgtgatgg cgaaaccttc cgcggtgatg tcgccgtcga
     2881 gcacggcatc gaagatgacg aactcgtcga gcctgatctg gcccacccga aggccgctca
     2941 gcttgagcgg catgaaaccc gatccgccaa cctcctcgtc agggaccact tcttcgagcg
     3001 cgaaaccctc gagatccccg gtgagcagca gatagtcggc tcggccatcc agaaccggcg
     3061 gcggctgacc aaagcccacc gcgccgcgct caggcgcgtc cgcgacgtgc agccaaccct
     3121 gggcggtacc gtggcggaag ctgatgatct gccgctcgtc cagaaactgc acggcaactt
     3181 caaccggtcg ggcgacctcc gcgggcttgc gcagatcggc cggaagatcc agcgccatgc
     3241 cctgaagatc ggagacgacc tccagccggg tgacgtgatc cggatccacg cccacgtgca
     3301 gcgaggccag gaagtcggcg ctgccggtca ccacgccagg atcttccatg tcgaggacgg
     3361 ccagcacgtc ctcgtgggtg gctcgcccag cgaagctgag gatcatctcc tgcgaattgc
     3421 tgtcggcatc gatgagaacg tcgttgccga acagggtacc gctgatccgg gaggacctga
     3481 gcccgttcgg ataccggtac cgcagatctc cctccagatc ccccagggcc agacggaaat
     3541 cgggcatccg gagcccggcg tccaacattc tgacgttgac gtccacctgc agctgctcgg
     3601 aggcgtccac gtcctcaggg agcttgatgg gaatgtgcag gttgccgagc acctcgagcc
     3661 ggccatcccc gacccactcc ggcgccacaa atcccatgga ctccgcaagg ggcgtggttc
     3721 gcacgaagtc cagcgccagg tcggtctcca ttcgagatac gagctccacg tccgcgtagg
     3781 aggcgttgtt gcgcagcacg acctgggagc cgcccagccg gacgccttcg cttgcgccct
     3841 tgtcgacggt gacacgcacc atcggaccgg cgaccacgac gtcggcgtcg agctcgtgga
     3901 cgtccggcca gtctggcgcg taggcaatgc ggccatcgct gatgcgggct gacagctcga
     3961 tccggcgggc cagctcgttc accttgaggt gctgctggcc gtgatagccg aaccgaatgc
     4021 gcgtcagcag gccggctttc agcccgccac gcagccagtc ctcgagaccc tcgtcgatgg
     4081 tgtacggcac gaacgtgaac gcctgatcgg tgtccagagc gtccacgccg atgagaagag
     4141 aaagccgctc ctcgtagcgc tcgccgggcg gccgcgtgag ggcaaagctg cccgccgccc
     4201 tgctgaacag ggtctcggcc cgcaggttga cgcctcgaag gccgagataa ccgttgccga
     4261 accaggcctg aaacgctccc tgcaggtagg gcatctgcca ggtatccgtg aaggtctggg
     4321 gaaacttgag gagcacgtcc tcgctcttca gcttcagctc cagcccccgg gcgtggccca
     4381 aaagctctcc cgccgccccg cgaacgaacg gggcaccgcg gtagccgtca acgtccaggc
     4441 cggccagcgt ggcggcaaac ccgctctctc ccgtgggcag tcgatagtaa cctcggacgt
     4501 tgagcgcttc ggcctgcagc ttgagagcgc tcaaccagcg gcgcgcgcgc tccgaacccc
     4561 tgagcgctcg ctcgagaaac tcggatacgc cacccatgtc cacccgttga gcccagaact
     4621 gaaccacttc cgcatctttg cggacgtgca cggcgggcat gcgcaatgcc cgctgcgtgc
     4681 gcacctgctc ctcggaatcg tccagatggg caaccagccc aaaggccagg tcgtgcacca
     4741 cggcgtcgtg aacgtcttcg aagcctctga cgtccgcgcg tacgagcccg gccaactcga
     4801 cacccccggg cagccgcagt cctcgcgcgt gggcgtgcag ccgcccgccg gattcgtcac
     4861 cgcgcaggtg ccagtcgatt ccgatactca tcaactccag agggctgtcc agcacggcag
     4921 ccggaatgac aaagccgcca ccgtccaacg aaagctgaat ccgctcgtcc cagaacggcc
     4981 agcggccgtc gcgggcctgg tactccaggt ggagcccgca gtccgcggcg cagtcggcgt
     5041 cctggttgtc gagatgcagt cgatggcgat gcaggccacc gcgattcgtg gccaggtagt
     5101 ccacctgcac cgactgtgcc tcttcctcac cggcaaggcc aaaagcgatg acgccggaga
     5161 agcgcagctc gtcggcgtga tagacgatgc cgctcgcgtc gaactcgcca tcccccgatg
     5221 tccccgccag gcgccatttc ccggccggcg tcttctcgag ccgcacggca ccctccccga
     5281 cgagcacccg ccgcgccacg aagcggttgc ggaccaggct ttccaaccag tcgagctcca
     5341 cgacgacttc ccggagatga ccggcgggga ggtccacgcg cgccgctcga accaccggat
     5401 tgaggtagcg ccacgaacct tccagacccg agacgcgaac gcgctggccg gacaaccact
     5461 ggttcgcgac cagttcgaga tccggcagga acatgaaccc caaccgcccg gtgccctgca
     5521 tcagcgcgac gaagagcatc gtcagcgtga ccacgaccag gaacggcctg agtacgattc
     5581 ggtgcaagta atggggtcct atgtgagatt agtttacgga agtcggcccg gaatcggcct
     5641 caaggcccgg ttcagcccct actacctttc gaccccgcat ttcaccttaa atttccgtgc
     5701 cgttcgtccg gtcagatcaa cattttcact gcgtatcgcc gacgaggacg atatcgaact
     5761 cgcccgcgcc gtagcttggt tccaattcga tacgcacccg gcgacccacg cgcgccgcca
     5821 tcgcggccag ccgatcggcg tcttcgtcca gtaacctgtc tacaacattg tccgctgccc
     5881 ggatgacgta gtcgccctgc gagtgagcgc cgcacccgct ttccgcatcc tgcatgatcg
     5941 agcggaaaat ctcgatgcac gtgctctggg ccgtcttcac caccccggcg ccgtgacagt
     6001 gcgagcacgg ctcgcacaac tgctgaacca ggctctccct ggtgcgcttc cggctcatct
     6061 gcaccagccc gagcgatgag aacccctcga tgcgaatccg cgccgggtcg ccctcgcagg
     6121 ccttctccag cgcgcgcagc acctgacggc gatgctcttc gtcttccatg tcgatgaagt
     6181 ccacgacgat gatgccgccg aggttgcgca ggcgaagctg tcgcgggagc acctgggctg
     6241 cttccagatt cgtgcggaaa acggtttcct ctaggttgtg cttgccgaga taggcgcccg
     6301 tgttgacgtc caccgtcgtc atcgcttccg tctgctcgat caccaggtga ccgccggatt
     6361 tgagttccac gcggctgccc agcgctcggg tcagctcgtc gtcaacgccg aagcgctcga
     6421 aaatgggtct cggctcgtcg tagagcacta ccttgtccgt caactccggc aggaactcgg
     6481 cgacgaattt ccgcacccgc tccaacgtcg gcgggtgatc gattcgtatg cattccagct
     6541 ccgccgtcgc catgtcgcga atcacccggg tgtgcagcgg caactcctcg taaacgatcc
     6601 cggggcagcc gcactcggct cgcctggcca tgatccggtc ccagatacgc aggaggacgc
     6661 gcacgtc
//
LOCUS       DBNHKV010000120         4323 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1858103, whole genome shotgun sequence.
ACCESSION   DBNHKV010000120 DBNHKV010000000
VERSION     DBNHKV010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1858103"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="AC16PP_03750"
     CDS             <1..889
                     /locus_tag="AC16PP_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR6480768.1"
                     /translation="WAYLTLPEVVDLPTTIPAVQAPSEPVPVPETTLAEPVPAAITPP
                     ARAPSLTSRDDGVDGAGSQAASPSETEPAPVPVPEIAPEIAASVAVEPDIRRPPMSSF
                     QSQRRAYAAVFDAWGADYRTAPPSMIPCNFAPSAGLQCLGLRGTWSDIEAMNLPVVLE
                     LWDSERAPYFAAVTHIHDGNMTLHLGDTVLTVTPKALRDVWFGQYVVLWQTPPQYRGS
                     ISRGNTHPTVSWLRVQLEALVSDDLSSGRPEYFDPRLDEAVRDFQGSEGLLTDGIVGP
                     ATWIRLAARLDLPAPKLDG"
     gene            892..1524
                     /locus_tag="AC16PP_03755"
     CDS             892..1524
                     /locus_tag="AC16PP_03755"
                     /inference="COORDINATES: protein motif:HMM:NF027851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HIR6480769.1"
                     /translation="MSYILDALKKSEIENDPAAAASLALTQQRESARNRTIAALVVVA
                     LLANAAVLLWIFRPDPGVPQEPVGPSVAAAQAEPVTVPGQRSEPVTPAPTPPEVSTPG
                     EVATTDVPVINPSPPQPPAREPARRPPARIRTQLADLPRDPKSRFPGLAFSTHVYAED
                     ALLRAVVANGTRLQEGDRLNEVTVEEITEDGVVVAFENYLVEIPVIESWQ"
     gene            complement(1551..2297)
                     /locus_tag="AC16PP_03760"
     CDS             complement(1551..2297)
                     /locus_tag="AC16PP_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480770.1"
                     /translation="MADRPPSEAISATPDDASGYRRGASGRRRGAVAEGPRMIGMSFI
                     MAVLVAGLVVAGWFIANQHQLLAAEQRALDDAEKRLVVLEDRLRVTDEAMTETGQDTR
                     EQIGFWESEIRKLWAVANERNRKWIKDNQVALGRLQKTLDELKGSNAELAGAVGRHET
                     AFSQQQAIIDQLAGIDLQMQRMVAGQRDLADKINVAQQTVAGLQSGLTGRVSENEQAV
                     AAIDAYRLQLNSRLADIERQLNQLMSSANL"
     gene            complement(2432..3103)
                     /locus_tag="AC16PP_03765"
     CDS             complement(2432..3103)
                     /locus_tag="AC16PP_03765"
                     /inference="COORDINATES: protein motif:HMM:NF017945.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HIR6480771.1"
                     /translation="MDQAFIDFLARWSHVVFGITWIGLLYYFNFVQTEYFKEADPDAR
                     TDAFSKLAPRALWWFRWGAMFTFLTGLIMLGVRGAGVTLSITLGSVLGTLMFLNVWLI
                     IWPNQKIVIASNQQVKAGGEADPAAAGAAPKAAIASRTNTLFSIPMLFFMVSSAHLAT
                     NTGIGFGMIGDASMTGLVVSLLIIAALELNGIFGKQGPMTTVRGVIGSSLVLTAVLWA
                     VAALL"
     gene            complement(3263..>4323)
                     /gene="groEL"
                     /locus_tag="AC16PP_03770"
     CDS             complement(3263..>4323)
                     /gene="groEL"
                     /locus_tag="AC16PP_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR6480772.1"
                     /translation="MQFDRGYLSPYFINNQDSMAAELEDPYILLCDKKISNIRDMLPI
                     LESVAKSGKPLMVIAEDIEGEALATLVVNNMRGIVKVSAAKAPGFGDRRKAMLQDIAI
                     LTGGTVISEEVGLTLEGATLDDLGTAKRISSTKENTTIVDGAGAKGDIEGRIKQIRSE
                     IEDTSSDYDREKLQERLAKLAGGVAVIKVGAPTEVEMKEKKARVEDALHSTRAAVEEG
                     VVAGGGVALIRALSAIEGLKGDNEDQNVGIAIALRAMTAPLRQIAANSGDEGAVVLDK
                     VRALTGNEGYNAATGEYGDMLAMGILDPAKVTRTALQAAASVAGLMITTEAMVAEAPD
                     DSPSPGMPGGMPDMGGMM"
BASE COUNT          757 a         1450 c         1354 g          762 t
ORIGIN      
        1 ctgggcctac ctcaccctgc ccgaggttgt cgatctgccg acgacgattc cggccgtgca
       61 ggccccgagc gagccggtgc cggtgcccga aaccaccctt gccgagcccg taccggcagc
      121 gattacgcca ccagcacggg ccccaagcct gacgtcgcgg gatgatggcg tcgatggcgc
      181 cggctcccaa gccgcgtcgc ccagcgagac cgagcccgcg ccggtgcccg tgccggagat
      241 cgcgccggag atcgcggcca gcgtagccgt cgagcccgat atccggcggc cgccgatgag
      301 cagctttcaa tcccagcgca gggcctacgc ggccgtgttc gacgcctggg gcgccgatta
      361 ccgcacagcc ccgccaagca tgatcccatg caacttcgcg ccgtcagcgg gcctgcaatg
      421 cctgggcctg cgcggcacct ggagcgatat cgaggccatg aacctgcccg tggtgctgga
      481 gttgtgggat tccgagaggg caccctactt cgctgcggta acgcacatac acgacggcaa
      541 catgaccctg cacctggggg acaccgtcct cacggtgacg cccaaagcgc tgcgcgatgt
      601 ctggttcggc cagtacgtgg tgctctggca gacgccgcct cagtaccggg gcagcatcag
      661 ccgaggcaat acccacccaa ctgtcagctg gctcagggtt cagctggagg ccctggtaag
      721 cgacgatctg tcgagcgggc gacccgaata cttcgacccc cggcttgacg aagcggtgcg
      781 cgactttcag ggttccgagg gcctgctgac cgacggcatc gtcgggcctg ccacgtggat
      841 ccgcctggcc gcgcgcctgg accttccggc tccgaagctg gacggctgac agtgtcttac
      901 attctcgatg cgctgaaaaa atccgagatc gagaacgacc cggcagcagc ggcgagcctg
      961 gcgctcaccc agcagcggga atccgcgcgc aaccgaacca tcgcggccct cgtcgtagtt
     1021 gccctgctgg ccaacgcagc cgtgctgctg tggatctttc gacccgaccc aggggtcccg
     1081 caagagccgg tcggtccatc ggtggccgcc gcccaggcgg agcccgtgac cgtgccgggg
     1141 caacgcagcg agccggtaac gcccgcgccc accccacccg aggtcagcac ccccggcgag
     1201 gttgctacca ccgacgttcc cgtcatcaac ccgtcgccac cccagccgcc cgcccgggag
     1261 cccgcacggc ggccacccgc aagaatcagg acgcagctgg ccgacctgcc gagagatccg
     1321 aagagccgct ttccgggact ggcgttctcg acccacgtct acgccgagga tgcccttctg
     1381 cgtgccgtgg tcgccaacgg cacccgcctg caggaaggcg accggctaaa cgaggtgacg
     1441 gtggaggaga tcaccgagga cggcgtcgtc gtggcgttcg aaaactacct cgtggagatc
     1501 cccgtcatcg agtcctggca gtaggggcgg cgaaaggctc tctcagaacg tcacaggttt
     1561 gccgaggaca tgagctggtt gagctggcgc tctatgtcgg cgagccggga attcagctgc
     1621 agccgatacg cgtcgatggc ggcgaccgcc tgctcgttct cgctgacccg gccggtaagc
     1681 cccgactgca ggccggccac cgtctgctgc gcaacattga tcttgtccgc caggtctcgc
     1741 tgtccggcga ccatgcgctg catctggaga tcgatgccgg cgagttggtc gatgatggcc
     1801 tgttgctgag agaaggccgt ctcgtggcgc ccaaccgcac cggcaagctc ggcgttcgac
     1861 cctttgagct catcgagcgt tttctgcagg cgcccgagcg cgacctggtt gtccttgatc
     1921 cacttgcggt tgcgctcgtt ggctacggcc cagagcttac gtatctcaga ttcccagaaa
     1981 ccgatctgct cccgggtatc ctggccggtt tccgtcatcg cttcgtcggt caccctgaga
     2041 cggtcttcta agaccacgag ccgtttctct gcgtcatcga gggcccgctg ctcggcggcg
     2101 agcagctgat gctggttggc gatgaaccat cccgccacga cgaggccggc caccagaacc
     2161 gccatgatga agctcatgcc gatcatcctg gggccttcgg ccaccgcacc ccgccgcctt
     2221 cccgaagcgc cgcgacggta tccggatgca tcgtccgggg tcgctgatat ggcctccgat
     2281 gggggtctgt ctgccatgtt ggtgtgtctc cagatacgct gaggccaatc ggccaggccg
     2341 gacggcggtt cagggcacgg aagttacagc aaaaaaaaga gcctgcaagt gcaggctctc
     2401 cgatctttca cccgacgggc cgtcgggccg cctacaacag tgcggccacc gcccagagaa
     2461 cggcggtcag caccaggctc gagccgatca cgccgcgaac cgtggtcatc ggaccctgct
     2521 tgccgaagat cccgttgagc tccagcgcgg cgatgatgag cagggaaacc acaagaccgg
     2581 tcatgctcgc atcaccgatc atcccgaagc cgatgccggt gttggtggcg aggtgcgcgc
     2641 tcgaaaccat gaagaagagc atggggatcg agaacagcgt gttggtccgg gatgctatcg
     2701 ccgccttggg cgccgccccc gcggccgccg ggtctgcctc gcccccggct ttgacctgct
     2761 ggttggaggc gatgacgatc ttctggttgg gccagatgat cagccagacg ttgaggaaca
     2821 tgagcgttcc gagaaccgac ccgagggtaa tcgagagggt cacgccggcg ccgcgaacgc
     2881 ccagcatgat cagacccgtg aggaaggtga acatggcgcc ccaccggaac caccacagtg
     2941 ccctgggggc cagcttggaa aaggcatccg tgcgcgcgtc aggatccgcc tccttgaagt
     3001 attccgtctg gacgaagttg aagtagtaca acaagccgat ccacgtaatg ccgaacacga
     3061 cgtggctcca gcgcgcgaga aagtcgataa aggcctgatc cattttgttt tctccccaaa
     3121 agcgatggat ttagcgtctc tctgagctcg aatatagcga aaccccctcc agaaatcacc
     3181 ttgaccatga ggctccccgg tccctggagc caggcaaaaa aaagcccctc ctggggaggg
     3241 gctttcgttc cagctcggaa ctttacatca tgccacccat gtcgggcatc ccgccgggca
     3301 tccccggaga cgggctgtcg tcaggggctt cggccaccat tgcctcggtg gtgatcatga
     3361 ggccggctac ggacgcggca gcctgcaggg ccgtccgggt cactttggcc ggatccagga
     3421 tacccatggc aagcatgtcg ccgtactcac cggtggcggc gttgtagcct tcgttgccgg
     3481 tgagtgcgcg gaccttgtcg agcaccacgg caccctcgtc gccggagttg gcggcaatct
     3541 ggcgcagcgg cgcggtcatg gcacgcaggg caatggcaat gcccacgttc tggtcttcgt
     3601 tatcgccctt gaggccttcg atggctgaca gagcgcggat gagcgcgacg cctccgcctg
     3661 caaccacacc ttcctcgacg gcggcgcgcg tggagtgaag cgcgtcctcg acacgggctt
     3721 tcttctcctt catttccact tccgtgggcg cgccaacctt gatcactgcc acaccgccgg
     3781 ccagcttggc cagacgctcc tgcagcttct cgcgatcgta gtcggagctg gtgtcctcga
     3841 tctcggagcg gatctgcttg atgcgtcctt cgatatcgcc cttcgcgccc gcaccgtcga
     3901 cgatggtcgt gttctccttg gtggaggaga tgcgcttggc ggtcccgagg tcgtccagcg
     3961 tcgcgccttc cagcgtcagg ccaacctcct cggagatcac cgtgccgccg gtcagaatcg
     4021 cgatgtcctg cagcatggcc ttgcggcgat cgccgaaccc gggcgccttg gccgcagaaa
     4081 ccttcacgat gccgcgcatg ttgttgacca ccagggtcgc aagcgcctca ccttcgatgt
     4141 cttcggcaat caccatcagc ggcttgcccg acttcgcaac gctctcgagg atcggcagca
     4201 tgtctcgaat gttggagatt ttcttgtcgc agagcaggat gtagggatct tccagctcgg
     4261 ctgccatgga atcctggttg ttgatgaagt aaggcgacag gtagccgcgg tcgaactgca
     4321 tgc
//
LOCUS       DBNHKV010000121         2781 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1151239, whole genome shotgun sequence.
ACCESSION   DBNHKV010000121 DBNHKV010000000
VERSION     DBNHKV010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1151239"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="AC16PP_03775"
     CDS             <1..822
                     /locus_tag="AC16PP_03775"
                     /inference="COORDINATES: protein motif:HMM:NF023203.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase CobT-related protein"
                     /protein_id="HIR6480773.1"
                     /translation="LVPLQHATSKLANRLQRKLMAKQSRTWEFDLDEGMLDASRLSQV
                     IIDPMNPLAYKQEKEMQFRDTVVTMLIDSSGSMRGRSITIAAMCADILGRTLERCSVR
                     VEVLGFTTRAWRGGQSREKWIASGKPSNPGRLNDLRHIIYKAADDPWQRTRRNLGLMM
                     REELLKENIDGEALIWAHNRLVVRHEERKVLMVISDGLPVDNSTLLVNPSNYLEQHLK
                     YAIDQIENYSSVELIAIGIGHDVTHHYQRAVTITDAEQLGGAMTEQLAELFEAKR"
     gene            complement(881..>2781)
                     /gene="leuS"
                     /locus_tag="AC16PP_03780"
     CDS             complement(881..>2781)
                     /gene="leuS"
                     /locus_tag="AC16PP_03780"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR6480774.1"
                     /translation="QWFFRITEYAQRLLDNLEHIDWSESTRKAQQNWIGRSEGATLRF
                     PVIEGGNGDPGTVDPLTVTADGDVIEVFTTRPDTLFGATFMVLSPEHPLVASITTDAQ
                     QAAVAAYQAQAAKTDLVSRQKADKEKTGVFTGGYCRNPANGAPIPVWIADYVLMEYGT
                     GAIMAVPGGDERDFEFATAMGIEIVRVVAGEGDGADTPLAEAYTGDGVLVNSGPFDGL
                     GVADGKRAVTAWLGERGLGELKVNYRLRDWCISRQRYWGPPIPIVHCDACGAVAVPED
                     QLPVELPRVDDFKPDDTGVSPLARVASWYETTCPGCDGPARRETDVSDTFLDSSWYFL
                     RYPSTDRNDAPMDDGITARWLPVDSYIGGNEHAVLHLMYSRFINMVLKDLGHIDFEEP
                     FVKFRAHGLITRNGAKMSKSRGNVITPDAIIDQYGADTFRTYLMFMGPLEEGGDYRDE
                     GIQGPFGFLHRLWDAVVTAKPDTAADEDVERKLHKTIKQATEQIPELGYNTAIAAMME
                     CLNAVRAGGRQPAVAEVEPLVVMIAPFAPHLAEELWERLGHGKSIFAGANWPAYDEAK
                     VRDEVVSVAVQVNGKLRGTISLAPGTDQAEAEALARAEENVARHLDNAEVRRVIYVPN
                     RLLNFVVG"
BASE COUNT          447 a          930 c          931 g          473 t
ORIGIN      
        1 ctcgtccccc tgcagcatgc cacctccaag ctcgccaacc ggctgcagcg caagctcatg
       61 gccaagcaga gccggacctg ggagttcgat ctggacgagg ggatgctgga cgcctcgcgg
      121 ctatcccagg tgatcatcga tcccatgaac ccgctggcct acaagcagga aaaggagatg
      181 cagtttcgcg acacggttgt cacgatgctc atcgacagct ccggatccat gcgcggacgg
      241 tccatcacca tcgccgccat gtgcgcggac attctgggcc gcaccctcga gcgctgctcc
      301 gtgcgggtgg aggtgctcgg tttcaccacg cgagcctggc ggggcggcca gtcccgggag
      361 aagtggatcg cctcgggcaa gccgtcgaac ccggggcgcc tgaacgatct ccgccacatc
      421 atctacaagg cggccgacga cccctggcag cgcacgcgcc gcaatctcgg cctcatgatg
      481 cgcgaggagc tgttgaagga gaacatcgac ggcgaggcgc tcatctgggc ccacaatcgc
      541 ctggtggtgc gtcacgagga gcgcaaggtg ctcatggtca tctccgacgg cctgccggtg
      601 gacaactcca cgctgctggt gaacccgagc aactacctag agcagcacct gaagtacgcc
      661 atcgaccaga tcgagaacta ctcgtcggtg gagctcatcg ccatcggcat cgggcatgac
      721 gtgactcacc attaccagcg cgcggtcacc atcacggatg cggagcagct cggcggtgcc
      781 atgaccgagc agctggcgga gctcttcgag gccaagcgct gacgggccgg gccctgggaa
      841 gccgacttca aacgggagcc tgcggtcccc gacacgacga ctaaccgacc acgaagttca
      901 gcaggcggtt gggcacgtag atgacgcgac gcacttcggc gttgtccaga tggcgggcca
      961 cgttctcctc ggcccgggcc agcgcctcgg cttcagcctg gtcggtgccg ggtgcaaggc
     1021 tgatggtgcc gcgcagcttg ccgttgacct gcacggccac cgagaccacc tcgtcgcgga
     1081 ccttggcctc gtcgtaggcc ggccagtttg cgcccgcgaa gatgctcttg ccgtgtccga
     1141 ggcgctccca cagctcctcg gccaggtggg gggcaaaggg cgcgatcatc accaccagcg
     1201 gctccacctc cgccactgcc ggttgcctgc cgccggcgcg cacggcgttc aggcactcca
     1261 tcatggcggc gatggccgtg ttgtaaccga gctcgggaat ctgctcggtg gcctgcttga
     1321 tggtcttgtg cagcttgcgc tccacgtcct cgtccgcagc ggtgtcgggc ttggcggtca
     1381 caaccgcatc ccacagccgg tgcaggaacc cgaaggggcc ctggatgccc tcgtcccggt
     1441 agtcgccgcc ttcctccagg gggcccatga acatgaggta ggtgcgaaag gtgtccgcgc
     1501 cgtactggtc gatgatggcg tccggggtga tgacgttgcc ccgggatttg gacatcttcg
     1561 cgccgttgcg ggtgatgagc ccgtgggcgc ggaacttcac gaagggttcc tcgaaatcga
     1621 tgtggccgag gtccttgagc accatgttga tgaaacgcga gtacatcagg tgcagcacgg
     1681 cgtgctcgtt gccgccgatg tagctgtcca ccggcagcca gcgggcggtg atcccgtcgt
     1741 ccatgggcgc gtcgttccgg tccgtggacg ggtagcgcag gaagtaccag gaggagtcca
     1801 ggaaggtgtc ggacacgtcg gtttcgcggc gcgccgggcc atcgcagccg gggcaggtgg
     1861 tttcgtacca gctggccacc cgggcgagcg ggctcacgcc ggtgtcgtcg ggcttgaagt
     1921 cgtcgactct gggcagctcc accggcagct gatcctcagg caccgccacc gcgccgcagg
     1981 catcgcagtg cacgatgggg atgggcggtc cccagtagcg ctgtcgggag atgcaccagt
     2041 cccggagccg atagttgacc ttgagctcgc ctaggccccg ctcgcccagc cacgcggtga
     2101 ccgcgcgctt gccgtcagcc acgccgagcc cgtcgaaggg gccggaattc accagcacgc
     2161 cgtcgccggt gtatgcctcg gccagcggcg tgtcggcccc gtcgccttcc ccggccacca
     2221 cgcggactat ctcgatgccc atggcggttg caaactcgaa atcccgctcg tccccgcccg
     2281 gcaccgccat tatggcgccg gtgccgtact ccatgagcac gtagtcggcg atccacacgg
     2341 gaatgggcgc cccgttggcc gggttgcggc agtagccgcc ggtgaaaacg ccggtctttt
     2401 ccttgtccgc tttctgccgg ctcaccagat ccgtcttcgc cgcctgggcc tggtaggcgg
     2461 ccacggccgc ctgctgcgcg tccgtggtga ttgaggcgac cagcgggtgc tccggggaga
     2521 gcaccatgaa ggtggcgccg aacagggtgt ccgggcgtgt ggtgaagacc tcgatcacgt
     2581 cgccgtcagc tgttacggtc agggggtcga cggtgcccgg gtccccgttg cccccctcga
     2641 tcaccggaaa acgcagggtt gccccctcgc tgcggccgat ccagttctgc tgagccttac
     2701 gggtgctctc cgaccagtcg atgtgctcca ggttgtccag caggcgctgg gcgtactcgg
     2761 tgatgcggaa gaaccactgg g
//
LOCUS       DBNHKV010000122         3811 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_230655, whole genome shotgun sequence.
ACCESSION   DBNHKV010000122 DBNHKV010000000
VERSION     DBNHKV010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_230655"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            330..1016
                     /locus_tag="AC16PP_03785"
     CDS             330..1016
                     /locus_tag="AC16PP_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR6480775.1"
                     /translation="MTNTGPATARPTDMGDAQQRLNATLPMARLEPVTLPDCSGMRLA
                     LINADFPTGPLPGEVMSAVVERPAYWAFCWGSGLGLAQFLLRNPEWVAGKSVVDLGSG
                     SGVAGIAAARAGAARVVACDTDADARLATSVNAAMNGVRLEVAATLPARCDLMLMADV
                     LYDRANLPLLSTAQRHADEVLVADSRIRELPDPEYVQVTTIDAATFPNLGEFDEFGTV
                     RVFHRPSARV"
     gene            complement(1013..1750)
                     /locus_tag="AC16PP_03790"
     CDS             complement(1013..1750)
                     /locus_tag="AC16PP_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480776.1"
                     /translation="MSRSLSWFLAFLVLLGVLAVAGSWYLGPSLVGLAFDADRREAPY
                     YVLSLAGRSELADADLRFGSDLAELFDAAGGKLLWRGTTERVVKGRLQDEWLNAQLFA
                     FPRGANFVELKTGGDYRDLLAAYEGRSRLLLGSPTQPDRMARGRIWVLNLIAEDAEDS
                     QATGGDPLAGLMGGVADFGGSLVWDAKVADLDERSSWNRLVLLGFAQPLEAEVWLSDA
                     GTQTELALSMERARRRVTLLMRPADPI"
     gene            1893..2726
                     /locus_tag="AC16PP_03795"
     CDS             1893..2726
                     /locus_tag="AC16PP_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6480777.1"
                     /translation="MYEEITYTVEDPVAIITMNRPDRLNAFTVRMLAEIRHALGAAEK
                     DGRVVGIVLTGAGRGFCAGMDMGSLDRMSSGGEAAAEDLSGLEAEVGDPGMGEDFQVT
                     YGYLMAVQKPVIAAVGGACAGLGFVIAMLTDMRFVEPQAKFTTAFSQRGLVAEHGVSW
                     ILPRLIGPGRALDILWSSRKFDGTEAERIGIAERLVDEGAAVSEACDYIRDLAANASP
                     TSLKMMKAQVYRHLNMSLGEAMRESNAWMAQSLKREDFTEGVRSFIERRPPRFKRVGE
                     D"
     gene            2758..3546
                     /locus_tag="AC16PP_03800"
     CDS             2758..3546
                     /locus_tag="AC16PP_03800"
                     /inference="COORDINATES: protein motif:HMM:NF014635.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HIR6480778.1"
                     /translation="MSDLITTDQTSYDWAELVAGLNRLLRLRTTPIGMKLFETRGEME
                     AVPRIRRPRDIHTTDQIVGQAARNGWTVGITAEDLVGAQCSTVIGLHPRSDDWLSGDR
                     MTGVWYETGEDASAHQHAMDVVPHGRYEAMAVSPLASGRLDPPDICLIYATPAQMILL
                     INGLQWSGYRKLEWGCVGESACADSWGRALATGEPSLSIPCFAERRYGGVMEDELLMA
                     MPPKFLPKTLAGLEQLSRNGLRYPIPQYGVNNDVRAGMGVSYGR"
     gene            complement(3562..>3811)
                     /locus_tag="AC16PP_03805"
     CDS             complement(3562..>3811)
                     /locus_tag="AC16PP_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480779.1"
                     /translation="LYLGELRQLTLRTVDGGTVPAAELIDGFWDGLVRWATVEQPQLA
                     AERQYAAIRTEILEHPDGERVLAAFDALSDPAGLASAG"
BASE COUNT          691 a         1322 c         1234 g          564 t
ORIGIN      
        1 ttacgagcca gttacacgcg atacaaagcc tcgactgcat ggcgccccat ctcccgctag
       61 gatactcggc ataatcagat aaaaaggtgg tgataacaac ggaccgcgcc ccgagaacca
      121 tcggtgacag caacccgaga cagcgcccta ccgcctgccc gggacgctcc caccgtaacc
      181 gagcgcatcc tcagtcaacg caccaggtat gaggtcacca tacccaccgc cggcgagcgg
      241 cccctccccc gctcgccggc aaccctcgtt caaaagattt cctccccgct agcctcgaaa
      301 cccggccccc gggcaaatat agtgcgccca tgacgaatac cggcccggca accgctcgac
      361 cgacagacat gggcgatgcg cagcagcggc tgaacgcgac cctgcccatg gcccgattgg
      421 agccggtgac ccttccggat tgctcgggca tgaggctcgc cctgatcaat gcggactttc
      481 ccacgggacc cctaccggga gaggtgatga gcgcggtggt cgaacgaccc gcgtactggg
      541 cgttctgctg gggcagcggt ctgggccttg ctcagttcct gctgcgcaat cccgagtggg
      601 tcgccgggaa atccgtcgtc gaccttggct cgggatccgg cgtggcgggg attgctgcgg
      661 cgagggccgg ggctgcacga gtcgttgcct gcgatacgga tgccgatgcg cggctggcca
      721 ccagcgtgaa cgcggcgatg aacggcgtgc ggctggaagt cgccgccacc ctcccggcgc
      781 gatgcgacct gatgctcatg gccgacgttc tctacgaccg cgcgaacctc ccgctgctct
      841 cgacggctca gcgccacgcc gacgaggtgc tggtggcaga ttcccgaata cgcgagctgc
      901 ccgatcctga gtacgtgcag gtcaccacca tcgacgcggc cacgtttccg aacctcggag
      961 agttcgacga gttcggcacg gtgcgggtat ttcaccgccc ttcggccagg gtctagatcg
     1021 gatcggccgg gcgcatcagc agggtgaccc ggcgccgcgc tcgctccatg ctgagcgcca
     1081 gctcggtttg ggtgccggca tcggaaagcc acacttcggc ctccagcggt tgagcgaaac
     1141 cgagcagcac cagacgattc caggacgagc gctcgtccag gtccgcgacc ttcgcatccc
     1201 agaccaggga gccgccgaag tccgcgacgc cgcccatcaa gccagccagg gggtcgccgc
     1261 cggttgcctg cgagtcctcg gcatcctccg cgatcaggtt gaggacccag atacggcccc
     1321 tggccatccg atccggttgg gtcgggctgc cgagcaacag acgcgacctg ccctcgtagg
     1381 ccgccagcag gtcgcggtag tcgccgcccg tcttcagctc cacgaagttt gcgccacgcg
     1441 ggaaggcaaa cagctgggcg ttcagccact cgtcctgcag acggcccttg acgacccgct
     1501 cggtggtgcc acgccatagc agcttgccac cggccgcgtc gaacagctcg gcaagatcgg
     1561 agccgaatct cagatccgcg tcggccagct cgctccggcc cgccaggctc agaacgtagt
     1621 agggcgcttc ccgacggtcc gcgtcgaacg ccagcccaac caggctgggg cccagatacc
     1681 agcttccagc aacggccaga acgccgagca gcaccagaaa tgccaggaac catgatagcg
     1741 agcggctcac cactgcaccc gagactttcc aaaagccgac atgataaacc atcgttcccg
     1801 ctgtgcagcg ccgccgtcaa ggcccgccag gcattactcg gctcgcggga tgcggtacat
     1861 tccatcggaa acggcccgct ccggagcgcc acatgtacga agaaatcacc tacacggtcg
     1921 aggatccggt cgcgatcatc accatgaacc gaccagaccg cctcaacgct ttcaccgtcc
     1981 ggatgctcgc cgagattcgg cacgcgctgg gagcggcaga gaaggacggc cgcgtggtcg
     2041 gcatcgtgct gaccggggcg ggccgcggat tctgcgccgg catggacatg ggcagcctgg
     2101 accgtatgtc ctcgggtggc gaggcggcgg cggaagatct ctccggcctc gaagccgagg
     2161 tgggggaccc cgggatgggc gaggacttcc aggtgaccta cggctacctc atggccgtgc
     2221 agaagcccgt catcgcggcg gtgggcgggg cctgcgcggg gctcggcttc gtcattgcca
     2281 tgctcaccga catgcgcttc gtggagcccc aggccaagtt cacgaccgct ttttcccagc
     2341 gcggcctcgt agccgagcat ggggtgagct ggatcctgcc ccggctgatc gggcccggcc
     2401 gggccctgga catactctgg agctcgcgaa aattcgacgg gacggaagcc gagcgtatcg
     2461 gcatcgccga acgcctggtc gacgagggcg ccgccgtcag cgaggcctgc gactacatac
     2521 gcgacctggc cgccaacgcc tcgccgacct ccctgaaaat gatgaaggct caggtgtacc
     2581 ggcatctcaa catgtcgctg ggcgaagcca tgcgcgagag caatgcctgg atggcccaga
     2641 gcctcaagcg cgaggacttc accgagggcg tgcgctcgtt catcgagcgc aggccgccac
     2701 gattcaaacg cgtgggcgag gactgactcc cgcagcgcgc caagacgagg accccccatg
     2761 agcgatctga taacgacgga ccaaacctca tacgactggg ccgagctggt ggccggtctg
     2821 aaccggctgt tgcggctgcg cactaccccg atcgggatga agctgttcga gacgcggggg
     2881 gaaatggagg cagtgccgcg catccgacgg ccgcgggaca ttcacacgac agatcagatc
     2941 gtcggccagg ccgcccgcaa cggctggacg gtgggcatca ccgccgagga cctcgtcggc
     3001 gcccagtgca gcacggtgat cggcctgcat ccgcgcagcg acgactggct ctccggcgac
     3061 cgcatgaccg gagtttggta cgagaccggc gaggatgcca gcgctcacca gcacgccatg
     3121 gacgtggtgc cgcatggtcg ctacgaggcc atggccgtga gcccgctggc cagcggcagg
     3181 ctcgatcctc ccgacatctg cctcatctac gcgacgccgg cccagatgat cctgctcatc
     3241 aacgggctgc agtggtcggg ttaccgcaag ctggaatggg gatgcgtggg cgagtccgcc
     3301 tgcgcggact cgtggggccg ggcgctggcc accggcgagc ccagcctctc catcccgtgc
     3361 tttgccgagc gtcgctacgg cggcgtcatg gaagacgagc tgctcatggc gatgccgcca
     3421 aagttcctgc cgaagacgct ggccgggctg gagcagctct cccgcaacgg ccttcgatac
     3481 cccatacccc aatacggcgt gaacaacgac gtgcgcgcgg gcatgggagt gagctacggg
     3541 cgctgacgcg tccggccaga atcagccggc cgacgccagg cccgccggat cgctgagggc
     3601 gtcgaacgcc gccaggaccc gctcgccgtc cggatgctcc aggatctcgg tccggatagc
     3661 agcgtactgc cgctccgccg ccagctgcgg ctgctccacg gtggcccatc gaaccaggcc
     3721 gtcccagaag ccgtcgatca gctcggcggc cggcaccgtg ccgccatcta ccgtgcgcag
     3781 cgtgagctgg cgaagctcgc cgagataaag c
//
LOCUS       DBNHKV010000123         2420 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_89673, whole genome shotgun sequence.
ACCESSION   DBNHKV010000123 DBNHKV010000000
VERSION     DBNHKV010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_89673"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="AC16PP_03810"
     CDS             <1..722
                     /locus_tag="AC16PP_03810"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose dehydrogenase family protein"
                     /protein_id="HIR6480780.1"
                     /translation="ELTKYAANVMLATRISLMNEIANLADLLGADIEAVRRGIGTDPR
                     IGYHFIYAGSGYGGSCFPKDVKALIHTASEAGYDAAIVRGVEAVNERQKSVLVGKLRR
                     HFDDDLEGRTFALWGLAFKPNTDDMREAPSCTIMEQLFAAGARVRAYDPAAMPEAAHL
                     YDGQDGLTLCEARDGTLEGADALVVVTEWNEFRSPDFLDVKARLKQPVIFDGRNLYDP
                     DYLAQLGFDYYAIGRGKPSQP"
     gene            805..1599
                     /gene="imuA"
                     /locus_tag="AC16PP_03815"
     CDS             805..1599
                     /gene="imuA"
                     /locus_tag="AC16PP_03815"
                     /inference="COORDINATES: protein motif:HMM:NF033429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HIR6480781.1"
                     /translation="MAEPSGKASRLLEHPALWRAGQLNQATEALSTGFPELDAHLPGR
                     GWPRAGLSEFLLDTTGAGELKLLAPLLKDLSRSEARWIAWVNPPFIPYAPALTALGID
                     VDRVLLIHPRDHKDALWAFERAARSGGCSVVLGWLDEKQLKVKDTRRLQLAARQGRAF
                     SCLFRPVRAAAESSMAELRLKMKALGSGKLEVSITKRRGGWPVTGLNLTVAEPRRPAE
                     IREQLSLWRSLQHPQDAGNEAELPVLGNPAPAGATHAPSSPGRLVH"
     gene            1599..>2420
                     /locus_tag="AC16PP_03820"
     CDS             1599..>2420
                     /locus_tag="AC16PP_03820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR6480782.1"
                     /translation="MLWLGLHLPNLGLEIFEAAAPGTPQVLVEDNRVILQNPAAVAAG
                     ITPGSTLATAHSIEPRLRHCNRDEIRERERLELLAGALYGFSSHVSTQPPSSLVLEAG
                     GSLELFGDLAKLEQAVRETCEALGHVTIMRTALTPSAAMTLARARQDDLKHVPLELTE
                     LATSMVERFANMGMHALGALIALPEDELAHRFGPELVNYLKRLTGRMPDPREAVTPAE
                     AFSSRLHLLEAVTGKDALLFPMQRLLGELQIWLVSRQLGAEQIVWTLDDHGSKDTA"
BASE COUNT          421 a          835 c          801 g          363 t
ORIGIN      
        1 cggagctcac caagtacgcg gcgaacgtga tgctggccac gcgcatcagc ctcatgaacg
       61 agatcgccaa cctggccgac ctgctcggtg ccgacatcga agcggtgcgc cgcggcatcg
      121 gcacggatcc gcgcatcggc taccacttca tctacgcggg cagcggctat ggcggctcct
      181 gctttccaaa ggacgtgaag gcgctgatcc acacggccag cgaggcgggc tacgacgcgg
      241 ccatcgtccg cggcgtggaa gcagtgaacg agcgccagaa gagcgtgctg gtgggcaagc
      301 tgcgcaggca tttcgacgac gacctcgaag gacgcacgtt cgccctgtgg ggcctcgcct
      361 tcaagcccaa cactgacgac atgcgtgagg cgccgagctg caccatcatg gagcagctgt
      421 ttgcagccgg tgccagggtg agggcctacg accccgccgc catgccggaa gccgcgcatc
      481 tttacgacgg gcaggatggc ctcaccctgt gcgaagcgcg ggacggcacc ctggaaggcg
      541 cggacgcact ggtcgtggtc acggagtgga acgagttccg cagccccgac ttcctggacg
      601 tcaaggcgcg gctcaagcag cccgtgatct tcgatggccg caacctctac gacccggact
      661 atctcgccca gctgggcttc gactactacg ccatcggacg gggcaagccg agccagccct
      721 gacgctccgg aatctcttga tactgtatat ccatacaggt aatctggccg acccctgtat
      781 gaaaagaccg tatcgagcgc gagcatggcc gagccgtccg gaaaagcctc ccgacttctt
      841 gaacaccccg cactctggcg tgccgggcag ctgaaccagg caaccgaggc gctcagcacg
      901 ggttttcccg agctcgatgc tcatctgccc ggccggggct ggccgagagc gggcctgagc
      961 gagtttctcc tggacaccac gggcgccggc gagctgaagc tgctcgcacc tctgctcaag
     1021 gacttgagcc gcagcgaggc gcgctggatt gcctgggtaa acccgccgtt catcccttac
     1081 gccccggccc tcactgcgct cggcatcgac gtggacaggg tgctgctcat ccatccgcgg
     1141 gaccacaagg acgctctgtg ggccttcgag cgggcggcgc ggtcgggcgg gtgcagcgtg
     1201 gtgctgggct ggctcgacga gaagcagcta aaggtcaaag acacccggcg gctgcagctc
     1261 gccgcccggc aggggcgtgc gttctcctgc ctgttccgcc cggtaagggc cgcggcggag
     1321 agctccatgg ccgagctgag gctgaagatg aaggctctcg gatccggaaa gctggaagtg
     1381 tcgatcacca aacggcgcgg aggctggccg gtcacggggc tgaacctgac cgtggccgaa
     1441 ccccgccggc ccgcagagat ccgcgagcag ctgtcgctct ggcgcagcct gcagcatccg
     1501 caggacgccg gtaacgaggc ggagctgccc gtcctcggca acccggcgcc ggcgggtgca
     1561 acccacgcgc cctcttcccc cggccgcctc gtccactgat gctctggctc ggcctgcacc
     1621 tccccaacct ggggctcgaa atcttcgaag cagcagcgcc cggcacgccc caggtgctgg
     1681 tggaggacaa ccgggtcatc ctgcagaacc cggccgcggt cgccgccggc atcaccccag
     1741 gcagcaccct ggccaccgct cacagcatcg agccgcggct gcggcactgc aatcgggacg
     1801 agattcggga acgggagcgg ctggagctcc tggccggagc gctctacggt ttctcctctc
     1861 acgtctccac ccagccgccg agctccctgg tgctggaggc aggcggcagc ctggagctgt
     1921 tcggcgatct ggccaaactg gagcaggcag tccgcgagac ctgcgaagct ctcggtcacg
     1981 tcacgataat gcgaacggcc ctgaccccct cggcggccat gaccttagcc cgtgcccgac
     2041 aggacgatct gaaacacgtc cccctggagc tcacggagct ggccacgtcg atggtggagc
     2101 gcttcgccaa catgggcatg cacgccctgg gagcgctgat cgccctgccg gaggacgagc
     2161 tcgcccatcg cttcggcccg gagctcgtga actatctcaa acggctgacc ggccggatgc
     2221 cggatccccg ggaggccgtg acgcccgcag aggcgttctc tagccggctg cacctgctgg
     2281 aggcggtgac gggcaaggac gccctgctct tccccatgca gcggctgctc ggcgagctgc
     2341 agatctggct ggtgagccgg cagctgggtg ccgagcagat cgtctggacg ctcgacgatc
     2401 acggatcgaa ggacacggcc
//
LOCUS       DBNHKV010000124         2333 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_230968, whole genome shotgun sequence.
ACCESSION   DBNHKV010000124 DBNHKV010000000
VERSION     DBNHKV010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_230968"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..868)
                     /locus_tag="AC16PP_03825"
     CDS             complement(32..868)
                     /locus_tag="AC16PP_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR6480783.1"
                     /translation="MSIDTEGAAAPHLLKLREQGNDTLALVRGEAVTELPKDLYIPPQ
                     ALEVFLETFEGPLDLLLYLIRRENLDILDIRVAEITAQYMSYIELMTAMQLELAGEYL
                     VMAAMLAEIKSRMLLPRPAYADEDEDDPRAELVRRLQEYEQIKNAAEGIDALPRLERE
                     IFIASAERPEVVKRHAEPDVDLKEVLVSLAEVLRRAEMFQHHEVYLEPLSVRERMTAI
                     LDRVNSASDFVSFGELFRAEEGRRGVVVTFLALMELVRESLLDFVQNEPYAPIYVRAA
                     GE"
     gene            complement(865..2079)
                     /locus_tag="AC16PP_03830"
     CDS             complement(865..2079)
                     /locus_tag="AC16PP_03830"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR6480784.1"
                     /translation="MSSNESSHRVLSGMRPTGRLHLGQYHGVIRNWLELQHGYECFFF
                     VADWHALTTGYEDTGNIEQNTFDMVIDWLAAGVNPGTATVFVQSWVPEHAELHLLLSM
                     ITPVSWLERVPSYKDQQARLNDRDLSTYGFLGYPVLQSADILMYRAGNVPVGADQVAH
                     VELTREIARRFNHVYGREPGFEDLAIAAIGKMGKKAARLYKSLRRGYVEQGDGEALER
                     ARALLAEQSNLSIGDRERLFGYLEGGGRIILPEPQALLNKAAKVPGLDGEKMSKSYQN
                     TIAMREAPDEVEKKVRTMITDPARVRRDDPGDPANCPVFALHEIYSDAETRAWVTEGC
                     TTAGIGCIDCKKPLIDAINAEQEAMRERAQQFEDDRDLVHSILVEGSEKARDVARETL
                     DDVRAAIGLAHR"
     gene            complement(2143..>2333)
                     /locus_tag="AC16PP_03835"
     CDS             complement(2143..>2333)
                     /locus_tag="AC16PP_03835"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR6480785.1"
                     /translation="TTTMRLADQDEPFSDPEQIRESLEHEVDLIIDGGPGGVVPTTVV
                     DLTGAVPEVIRQGLGVFE"
BASE COUNT          398 a          779 c          701 g          455 t
ORIGIN      
        1 tctcgcatcg tccacagctt cgttcatggg ctcattctcc agccgcgcgc acgtaaatgg
       61 gcgcgtacgg ttcgttctgg acgaaatcca gcagggattc gcgcaccagc tccatcagcg
      121 ccaggaaggt gacgaccaca cccctgcgcc cctcttcggc tctgaacagc tcgccgaacg
      181 agacaaagtc gcttgccgaa ttaacccggt cgaggatggc cgtcatgcgc tcgcgtaccg
      241 atagcggctc caggtacacc tcgtgatgct gaaacatctc ggcccgccgc agcacctccg
      301 ccagcgaaac gagcacctcc ttgaggtcga cgtccggctc cgcgtggcgc ttcacgactt
      361 ccgggcgctc ggcactggcg atgaatatct ctctttccag tcgcggcagg gcatctatgc
      421 cctctgccgc gttcttgatc tgctcgtact cctgcagccg gcggaccagc tccgcacggg
      481 ggtcgtcttc atcttcgtcg gcataagccg gacgtggaag cagcatcctc gatttgatct
      541 ccgccagcat ggcggccatc accaggtact ctccggccag ctccagctgc atggccgtca
      601 tcagctcgat gtagctcatg tactgcgcgg tgatctcggc gacccggatg tccagaatgt
      661 cgagattttc cctgcggatc aggtacagaa gcagatcgag cgggccctcg aacgtctcga
      721 ggaaaacctc gagcgcctgc ggaggaatgt agagatcctt gggcagctcg gttaccgctt
      781 cgccccgaac gagcgccagg gtgtcgttgc cctgctcacg aagcttcagc agatggggag
      841 cggcagcgcc ttccgtgtcg atgctcatcg gtgggccagt cctatggcag cgcgcacgtc
      901 gtcgagagtt tcccgcgcga cgtcgcgggc cttttcggag ccttccacga ggatggagtg
      961 caccagatcg cggtcgtcct cgaattgctg cgcccgttcg cgcatggctt cctgctccgc
     1021 gttgatggca tcgatcagcg gcttcttgca gtcgatgcag ccaatccccg cggtggtgca
     1081 gccttcggtc acccaggcgc gggtctcggc gtccgagtat atctcgtgca gcgcgaatac
     1141 gggacagttc gccggatcgc ccggatcgtc acggcgaacg cgcgccggat cggtaatcat
     1201 ggtgcggacc ttcttttcca cctcgtcggg agcttccctc atggcgatcg tgttctggta
     1261 ggacttggac atcttctccc cgtccagccc gggcaccttc gccgccttgt tcaacagggc
     1321 ctgaggctcg ggaaggatga tacgcccacc accttcgagg tagccgaaga gccgttcgcg
     1381 atcgccaatg gacaggtttg actgctccgc cagcagggct cgcgctcgct cgagggcctc
     1441 gccgtccccc tgctcgacgt atcctcgtcg caggcttttg tagagccgcg cggccttctt
     1501 gcccatcttg ccaatggccg ctatcgcgag gtcttcgaac cccggctccc gcccgtagac
     1561 gtgattgaag cggcgggcga tctcccgggt cagctcgacg tgtgccacct gatcggcacc
     1621 cacgggcacg ttgcccgctc tgtacatcag tatgtccgca gactggagaa cggggtaccc
     1681 gagaaagccg taggtcgaaa ggtcgcggtc gttcaggcgg gcctgctggt ccttgtagct
     1741 cggcacccgc tcgagccagc tcaccggggt gatcatcgac agcagcagat gcagctcggc
     1801 gtgctcgggc acccaggact gcacgaagac ggtggccgta cccgggttga cacccgcagc
     1861 gagccagtcg atcaccatgt cgaaggtgtt ctgctcgatg ttaccggtgt cttcgtagcc
     1921 ggtagtcaac gcgtgccagt ccgccacgaa gaagaaacat tcgtagccgt gctgcagctc
     1981 gagccagttc ctgatgacgc cgtgatactg gccgaggtgc agccggccgg tcggacgcat
     2041 ccccgagagt acccggtgac tcgactcgtt actgctcaac gcagcctctc aactacaaac
     2101 tgaggcggca ttatagggct ggggcccggc aaaaccaccc gttcactcga acacgcccaa
     2161 gccctgccgt atcacctccg gcaccgctcc ggtgaggtcg acgactgtgg tgggcaccac
     2221 gccgcccggg ccgccgtcga tgatgaggtc cacctcgtgc tccaggctct cccggatctg
     2281 ctccggatcc gaaaagggct cgtcctgatc ggcaagccgc atggtggtcg tca
//
LOCUS       DBNHKV010000125        22125 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_159876, whole genome shotgun sequence.
ACCESSION   DBNHKV010000125 DBNHKV010000000
VERSION     DBNHKV010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 22125)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22125)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22125
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_159876"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="AC16PP_03840"
     CDS             complement(<1..286)
                     /locus_tag="AC16PP_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6480786.1"
                     /translation="MIFRQLFDADSSTYTYLLGCADTRDAVIIDPVFEQHGRDCALIR
                     ELGLKVRYVLDTHVHADHVTSAWLMHSSLDAEIVLSRRYGIEGVDVAVDHG"
     gene            352..1521
                     /locus_tag="AC16PP_03845"
     CDS             352..1521
                     /locus_tag="AC16PP_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013624.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I family protein"
                     /protein_id="HIR6480787.1"
                     /translation="MYTPFAAATERVPNFAAPGLIIRSQGPSAPDPETQRLPRAIASL
                     PGVADRHLNLAKQLERQLARFTDAGQADATVAESARTLWRAAVSDVQNGLDDDRPLYW
                     GRLALRRSVYRSALREKDSLLRSIEHYSRGMGDLRLGDDVLNVLITGFDPFHLDTRLD
                     QSNPSGLAALALNGSEFGSGGGRARVCASIFPVRFEDFDDGIVERFFQQILAEREPAM
                     VVTLSMGRDAFDLERFPGRRRSAQDPDNHNVLTGASPDKPLPPGKTGSHLEGPEFLEF
                     SLPVHAMRAADGHWPVRDNRWVTTIERGEFEAGSLADLQSQLAVRGSGGGYLSNEIAY
                     RSRLLHGQLGCSCPIGHVHTPALQGHNAALERLMVGQIRQMLAQAAASLTPRPPS"
     gene            complement(1518..2540)
                     /locus_tag="AC16PP_03850"
     CDS             complement(1518..2540)
                     /locus_tag="AC16PP_03850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019471932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6480788.1"
                     /translation="MMNVNRQWVLAKRPYGMVTKDNFEYREETIPEPGDGEVLIRNLY
                     LSFDPTQRGWMEDRESYLPPVQIGEVMRAGSVGQVVESRSDDFSAGDLVQTTGGWQDF
                     TVMSAGMGPMGLRKLPEGVTPRLALGALGVTGLTAYWGMLDLGQPKAGETVLVSGAAG
                     ATGSVAGQIARIKGCRVVGIAGGPEKCAWLKDEARFDDVIDYKNEDVDRRIGELCPNR
                     VDVFFDNVGGGILEAALNHINMRARVVLCGGISAYNATEPQPGPVNLMNLVIMRARME
                     GFIVIDYMDRSHIAVPELLGWINSGEIVHQEDVQEGFENIPDTLNRLFTGRNLGKQLL
                     KIADPS"
     gene            2619..4073
                     /locus_tag="AC16PP_03855"
     CDS             2619..4073
                     /locus_tag="AC16PP_03855"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6480789.1"
                     /translation="MSDIAFLSATELARRIRDRELSSEELLLHYLQRVDRYNGALNAI
                     VVDIREEALMAARAADAAVARGVDVPPFHGVPMTVKESYNVAGTPTTWGNPKWRGNVA
                     FEDAESVKRLKDAGVIIFGKTNVPLALADFQSFNAIYGTTNNPFDHSRAPGGSSGGSA
                     AALAAGLTGLEAGSDIGGSIRNPAHFCGVFGHKPTWNLLWMRGHAPPGDVRASSDISV
                     IGPLGRSAADLETAVRTMAGPDDIMARGYTLSLPTLKRGGLGGLRIAIWEDDPVCPVS
                     AETKARVLAVASACRDAGAEIIEGARPDFSSEHSHETYQNLLQPTMASRMPDEEYESL
                     LEYVANLDPRDTSRTARVLRAQVARFRDWKRSHELREHLRWKWHLFFQDYDLMLTPMM
                     PTSAFPHDHRPISQRTIEVDGTSMPYMDQIFWAGLTGVAYLPSTVIPTGLDDSGLPIG
                     VQLVGPEYGDLITIGVARELETAGFRFVAPPAYA"
     gene            4086..5030
                     /gene="mvaD"
                     /locus_tag="AC16PP_03860"
     CDS             4086..5030
                     /gene="mvaD"
                     /locus_tag="AC16PP_03860"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HIR6480790.1"
                     /translation="MGTTARAHPNIALIKYWGKAPGQENIPATPSLSITLDSLVTETH
                     VATGPSRDELYFDDEPVADRKVLDWLEVLRQRYDIPPLEIRTANNFPTAAGLASSASG
                     FAALAVALDAQCGLQLDAATLSDLARRASGSAARSIFGGYVAVQGPRWVAQPVLSPEA
                     WPLKTVIAVTTTARKSVSSSEGMAISAATSPYYPGWVESTRADFTDALAAVRTRDFQA
                     LAELAEFSCLKMHGLMLGSRPGLLYWKAATVSCLHAVRALRQDGVDVFFTVDAGPQVK
                     AICKPSAESDVRAALAAVPGVEQIVVTGLGSGARVIDP"
     gene            5027..5998
                     /locus_tag="AC16PP_03865"
     CDS             5027..5998
                     /locus_tag="AC16PP_03865"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase family protein"
                     /protein_id="HIR6480791.1"
                     /translation="MIHASAPGKVVLWGEYAVLTGAPGLVLAVDRRASCTLTPGGDTW
                     HFETRGLPEPAVDLDVADLTGAHPPGPSGGALAWHVLRHLDSEVPAGATLTTDTASFQ
                     DGGHKLGIGSSAAVCTAVYGAFCELLGIGPSLNDALAVHRSLQGGSGSGIDVAAAYLG
                     GSLRYQLRGGARPPAAEPFDLPDGLILRFIWTGTPAQTTRHVARFEAWRAENQTSTLT
                     ELAQASAALFESGHLVDDLAEYAERLYAMDRAAGLGIYTDAHLQLSQVAKECKVVYKP
                     CGAGGGDIGVAASNNEYRVERFVSAALGLGFQIVEMEKTEHGIQLAS"
     gene            5976..8225
                     /locus_tag="AC16PP_03870"
     CDS             5976..8225
                     /locus_tag="AC16PP_03870"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HIR6480792.1"
                     /translation="MASSSPAESGSRIPNFFRMSVAERIAALHARGLLSSEDVHRLTA
                     GDHTLRLPVADKMIENVVGVFGLPMGVALNFLVNGRDYVVPLVVEEPSIVAGLSGAAR
                     LARLGGGFRASSTEPILIGQVQVVNVDDPDKAMADLLANRDEILALANSLHPKMVARG
                     GGALDIEVFHHHAPEDGREMVVLHLLVDTRDAMGANLVNTMCEGIASMVETITDGKVF
                     LRILSNLTDRAVATASVRIPAQNLEGKGFSGEDVRDGIILAADLATVDPYRAATHNKG
                     VMNGVDAVAIATGNDWRAIEAAAHAYAARDGQYRSLTRWYKNEDGDLVGEIQIPMKVG
                     TVGGSLETNPSVRINHRLLGTPGATELAGIMAAVGLAQNFAALRALSTDGIQQNHMTL
                     HARSVASTANVPEELFDTVVEALIESGEIKVWKAQEIVKNLTRKIAMPADVERSSACG
                     KVILLGEHAVVYGRPALAVPIPLAVEASVRRGGGDGINLVIPRWGLEQKVRASNAQGL
                     SGILYHVLEQLGLDQENMTIEVIPHVPRAMGLGGSSANAVAILRALDHTFRLRLSDGR
                     LNELAYECETGAHGTASGIDNTIATYGAPLVFERGEDGPRFEEIRVAEPLPLVIGITG
                     KECLTANTVALVRRAWEAHQARYDGIFDQIATLTRAGVDAMRDAHFAELGELMNLCQG
                     YLNALQLSTPELEELIHTARANGALGAKLTGGGGGGSMVALCPDSQDQVARAMEAAGY
                     QALKVTIQP"
     gene            complement(8227..8925)
                     /locus_tag="AC16PP_03875"
     CDS             complement(8227..8925)
                     /locus_tag="AC16PP_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480793.1"
                     /translation="MDEAYRLVFRGEFLEGQHPAVARKRLVQALQLNDAQAEKLFSGH
                     PVVVKKRADAATAARYQDLFKQAGARLRVMPLASEAPAAEETTATDAGASSGASALEV
                     MPSGSDVLRADERSTFEELEIDLSHLAVLDWAPEVPDSAAPEIVAPDYSVADLGVDLA
                     ERISREHPGIKADFDLAEPGSLIPNLAVDTAPVVDVAAIEFDVAEVGADIGDPSDDAD
                     VTSPDVSHLSMAVE"
     gene            9172..10029
                     /locus_tag="AC16PP_03880"
     CDS             9172..10029
                     /locus_tag="AC16PP_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010562866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HIR6480794.1"
                     /translation="MSINIGKFEYPAHPKLTAHPTLWKVLPDARELVTANVERLSELQ
                     ERLYANNRYSVLMIFQGMDAAGKDSTIKYVTSGVNPMGFQVVNFKQPTYKELDHNYLW
                     RYWRAMPERGRIGIFNRSHYEEVGVARVHPEIIERQNLPSEHVNGDFWENRFEDIRAM
                     ERHLHTNGTIVLKFFLNVSKKEQKARLMKRLTNRDKHWKFDPRDIDERVHWDDHMKAY
                     EAAIAATHFDHAPWYVIPADDKWTMRVIVAEVLCTHLEQLRLSYPPAKGAHKHALAEA
                     LSRLEAEKE"
     gene            complement(10032..11192)
                     /locus_tag="AC16PP_03885"
     CDS             complement(10032..11192)
                     /locus_tag="AC16PP_03885"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6480795.1"
                     /translation="MIEIHGYCDERFSPVREVFARNFADKDEVGASLALTVEGEMVVD
                     MWAGHRDAAKSLPWEQDTIVNVWSTTKTMSALCALVLADRGEIDLYAPVTRYWPAFGQ
                     NGKEKIEVRHFMSHSAGLAGTDEPIRGEALYDWERVVNALERQAPWWEPGTASGYHAI
                     TQGHLIGEVVRRVTGMSCGTFFRREIAEPLGADFHIGTAPEHFSRIGDLLPPGVSLGG
                     ALAHEPSGRGGGEGRAASIAVRAFRSPFVRAEDAATAEWREAEIPAANGHGNARSVAR
                     IQTVIANGGVSAGTRILSEAGCRAVFDEQTHGRDLVLKAPLTFGMGYALLHGQSPREL
                     AFWGGWGGSSVVVAPQSRMCIAYVMNRMEPSLLGDDRGVSLNRAALAVMGRG"
     gene            complement(11219..11662)
                     /locus_tag="AC16PP_03890"
     CDS             complement(11219..11662)
                     /locus_tag="AC16PP_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6480796.1"
                     /translation="MSYYLRPGLPQPAPLPDGLDRSYWDGLREDRLMLQRCAQCGGWQ
                     WGPEWICHHCLGFELAFEAVTPRGIVYSYERVWHPVHPALAEQGPYLVVLVEIPQADG
                     VRLVGNLLGDPLQDVEIGSAVEAVFEHHEDVDEPFTLLQWRLLAD"
     gene            complement(11659..12837)
                     /locus_tag="AC16PP_03895"
     CDS             complement(11659..12837)
                     /locus_tag="AC16PP_03895"
                     /inference="COORDINATES: protein motif:HMM:NF047238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR6480797.1"
                     /translation="MNRSLRGRVAVVGVGETMYYKAGQSPDAEFKLALKAILAACEDA
                     GIDPKDVDGFASYSNDRSDPSRLAAALGCRELRFANMQWGGGGGGGSGALGNAAAAVA
                     TGMADCVVVFRALAQGQFGRFGQGPRRNVIAGDMAHVVPYGLMSPAQMFAMKVQRFMH
                     DHGVEQSALRAISLASYHHAQNNPRAVMHGRPLDEATYDASRWIVEPFHLYDCCLEND
                     GAAAMIVVPADRAGDCRPVPAYLLGAVSGSHYRAGASVHNTPDYATSTFKTLAPRLYE
                     MAGVVPADVDVLQSYENFTGGVLMSIVEHGFCEAQRCNEFFVPERLTAPDGDLPLNTS
                     GGNLAECYMHGLGLNIEAVRQIRGESTNQVMGAEISMVISGPMVTPVSACILGSEAAL
                     "
     gene            complement(12862..13497)
                     /gene="amrA"
                     /locus_tag="AC16PP_03900"
     CDS             complement(12862..13497)
                     /gene="amrA"
                     /locus_tag="AC16PP_03900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HIR6480798.1"
                     /translation="MARLPSTDYGPDARQALLGIARDAIRIGLERHGVADPMSVVDVP
                     SLLPELLEPRATFVTLTQAGSLRGCTGSLEALRPLALDVVENACHSAFRDPRFPPVQA
                     GELNRLRIELSVLSPLERMRVASEGELLERLEPSMGLVIEEGGRRGTFLPKVWESLPD
                     PRVFLDELKAKAGFPAGHWSDATEVFYYRTQTFAEPVAAVGSVVSHGGDSV"
     gene            complement(13475..14260)
                     /gene="amrB"
                     /locus_tag="AC16PP_03905"
     CDS             complement(13475..14260)
                     /gene="amrB"
                     /locus_tag="AC16PP_03905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HIR6480799.1"
                     /translation="MVSRSPAVADLFYPGDPAELAGTVRAYLDRADAPTVALRPKALI
                     VPHAGYAYSGPVAATAYRTLEPFRHEVERVILMGPAHRVYLEGLAVPETDAFAVPTGN
                     VPVDVEAVARLLELPGVLASDQAHAAEHCLEVHLPFLQAVLDRFSIVPVVVGACSSEL
                     VADVLDLLWGGDETLIVVSSDLSHYHPYEVAQALDAATAGEILARACNLTGEQACGAV
                     AINGLMLAATGHDLQVRELDLRNSGDTAGDRDRVVGYGAFALD"
     gene            14321..15397
                     /gene="amrS"
                     /locus_tag="AC16PP_03910"
     CDS             14321..15397
                     /gene="amrS"
                     /locus_tag="AC16PP_03910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HIR6480800.1"
                     /translation="MDQTIVPTRYWHTLDDGRTQCDVCPRHCRLREGQRGLCYVRQCL
                     DGEVKLVSYGRSSGFCVDPIEKKPLNHFYPGSSVLSFGTAGCNLACKFCQNWDMSKSR
                     EMDTLADRAMPERLAEAAVDLACRSVAFTYNDPVIFLEYAVDVADACREVGVKSVAVT
                     AGYVDPEPRREFFSAVDAANVDLKAFSERFYHKICGGHLAPVLETLEFIRHETNTWLE
                     LTTLLIPGENDSDEELDEMTGWVVEHLGPDVPMHFTAFHPDWKMRDVPSTPPGTLTRA
                     RSIARRNGVHFAYTGNVHDPAGSSTYCSACDALLIERDWYQLGRWGLDGAGRCSACGE
                     PLAGHLEGPPGQFGSRRIPVRLDQ"
     gene            15488..15760
                     /locus_tag="AC16PP_03915"
     CDS             15488..15760
                     /locus_tag="AC16PP_03915"
                     /inference="COORDINATES: protein motif:HMM:NF040598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpp/OprI family alanine-zipper lipoprotein"
                     /protein_id="HIR6480801.1"
                     /translation="MKNLFFAMIATLLLAGCATTEQVEALQNRVSALENQIGSANDAA
                     GDAASAASRAASDAAAAQSTAERALDAANAANERAERISETCCSRK"
     gene            complement(15768..16664)
                     /locus_tag="AC16PP_03920"
     CDS             complement(15768..16664)
                     /locus_tag="AC16PP_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR6480802.1"
                     /translation="MSVPTSRTSAAALLACLLVAAAATEAASYRLPEGGEDVIGASIS
                     LTLAYEDTFAGVAQQFGVGYQELVDANPGVDPWLPGDGARIVMPTAYVLPSAPREGIV
                     INVAEYRLYYYPPGRGSVITFPVGVGRMDFPTPLISTKVVTRIENPSWTPTDAARREH
                     AEMGDPLPHVVPPGPDNPLGHLAIQLSAPGYFIHGTNKPFGVGQGVSHGCVRLYPGHI
                     ETLSELVPNGTPVYIVNEPYKVGWRDGELWIEAHRNLYGENQASELVALIVDATEKRD
                     ASVDWRRVDEVSAALTGIPVRL"
     gene            16812..17651
                     /locus_tag="AC16PP_03925"
     CDS             16812..17651
                     /locus_tag="AC16PP_03925"
                     /inference="COORDINATES: protein motif:HMM:NF046782.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR6480803.1"
                     /translation="MPINPDAVGAEGRPVRRSWSSKDALLYAVGVGSGTDELAFTTEN
                     TKDVAQRVLPTFAVIIGGGGVPMDAIGSFNPALMVHGEQGIELYDEIPPDGEIDSVGK
                     VTAIWDKGSAAVLEFESTSTNVATGKPLLKTRTSLFCRGEGGWGGDRGPSEKVEFPDR
                     EPDHQVTYATREDQALTYRLSGDRNPLHSDPSFAALGGFEKPILHGLCTWGFTGRALL
                     HTLCDGDPSRFKSMNSRFSKPVIPGDELTISIWVVDEGRALFRTTNQNGDAVIDQGVF
                     TYA"
     gene            17677..18069
                     /locus_tag="AC16PP_03930"
     CDS             17677..18069
                     /locus_tag="AC16PP_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480804.1"
                     /translation="MIELVLRLSLWISLPLNAGAAYILAFPSSWPGQLLGLPAAPDPV
                     YAYLAAYLVALLGLAYGWMALQPSIVRPLLWLGALGKGGAFAVAAALWLAGLARAPLA
                     AAVTVDLLLAAFWIAWLTGTRGKASADG"
     gene            complement(18123..19517)
                     /locus_tag="AC16PP_03935"
     CDS             complement(18123..19517)
                     /locus_tag="AC16PP_03935"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6480805.1"
                     /translation="MTDLHYLSLAGVCRRMKSGELSASAVTEAILARIEALDSNLNSF
                     ATVMADEARAVAARLDQDRADGKPLGALHGVPVAVKDLFDTRGVVTASGTRVMGERVP
                     DEDATVVRRLKDAGAVIVGKTQLTEGAYGTHHPDVDPPKNPWDGDYWPGVSSSGSGVA
                     VAAGLSFGALGSDTGGSIRFPSACCGLVGIKPTYGRVSRYGAFPLAESLDHVGPMARS
                     VEDAARLLQVLAGADPYDVTTQPDPVPNYAASVADTLPGLRIGVDWDYVSKGVDEPVV
                     ETIREALEIFRELGATVVDIEVPASAATLVRGWGVTCGMECARAHRDFYPDRKAEYGP
                     VLAAFIDYGRAVSREDYGLLETVRAEFRDGLNGCLESADVIIAPCMPELPPPADALAD
                     GPRRPSDRADLITFTAPFNYSGHPTITLPAKLERGSLPRAFQLIARPLGEPTLVRAGS
                     AFESALGLDAHPIP"
     gene            complement(19514..20911)
                     /locus_tag="AC16PP_03940"
     CDS             complement(19514..20911)
                     /locus_tag="AC16PP_03940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6480806.1"
                     /translation="MTDVVVAELIEALGEDCVLTGEDVHSRQAGIWRSDTIQAKALVR
                     PRTTAEVSRTLAICNAHGQSIVPHGGLTGLVESAITGPDDVALSLERMNRIEEINPVD
                     RTMVVQSGVILQVLQEAAEEHQLMFPLDLGGRGSCTIGGNISTNAGGNRVIRYGMTRD
                     MVLGLEAVLADGTVLSSMNQMIKNNAGYDLKQLFIGTEGSLGVVTRAVLRLREQPACR
                     ATLLVSLDEFAKLAQFLKHMDAALGGALSAFEVMWNNFYTLVTTPPAENQAPIPRDYP
                     YYALVEAMGSDDAAVEAALAEAYEKSLITDAVIAQSEAQRLALWALRDSVEHTARYAP
                     IYTFDVSLRISHMESYLAEVNARLEARYQDVNNFTFGHMGDGNLHLVISVGEGGPETR
                     RAVEACVYEPLAAIQGSVSAEHGVGLEKKPYLGISRSEQEIELMRTIKRALDPKGILN
                     PGKIFDSASVNEAAA"
     gene            21052..21648
                     /locus_tag="AC16PP_03945"
     CDS             21052..21648
                     /locus_tag="AC16PP_03945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6480807.1"
                     /translation="MARTFNKAYYDRYYRDPRTRVTTPAGTRRQAGFIAAYLDYLELR
                     PRSIVDLGCGTGTLLRALGKHFPRARLTGVEVSDYLCRRHGWLQGSVADFQAEHPFDL
                     VVCNDVLAYLDDAGCNRALRNLASLTGAALFLGVLTEEDLKLCDRRRTDPSQHARPAA
                     WYRRRLNRHWANVGGGLYLKRPLGVTVWTLDALNTVPS"
     gene            complement(21645..>22125)
                     /locus_tag="AC16PP_03950"
     CDS             complement(21645..>22125)
                     /locus_tag="AC16PP_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480808.1"
                     /translation="AALKSFIAKYGFKEGLFPTSRDLLDEFRAVAGAEHQPLITDLFE
                     RITVYDLAVTDASVAENDGGFDVTITVAARKLDADGEGREAEVPLSQMFDLAVFPEAP
                     EDLSEDDLPDPLLAERRQVVSGEQTFTVRVDERPERVGIDPYNKMIDRNPDDNLRRL"
BASE COUNT         3980 a         7426 c         7003 g         3716 t
ORIGIN      
        1 cgccgtggtc cacggcgacg tcgacgcctt cgatcccgta acgtcgcgag agcacaatct
       61 cggcatcgag ggaactgtgc atcagccaag cactggtcac atgatccgca tggacgtggg
      121 tgtcgagcac gtacctgact ttcaaaccga gctctctgat gagcgcgcaa tcgcggccgt
      181 gctgctcgaa gacggggtcg atgatgaccg catcgcgagt atccgcgcac ccgagcaggt
      241 aggtatacgt ggacgagtcc gcatcgaaaa gctgtctgaa tatcatgacg ttccctcacc
      301 ctaccggtgc gctcaaagtc taccccgcga gccgatacct cgcatcctga cgtgtatact
      361 ccctttgccg ccgccacgga gcgggtgccg aatttcgcgg cgcccggact catcattcgt
      421 agccagggcc cttctgctcc agaccccgaa actcaacgtc tgccccgggc gattgcctcg
      481 ctcccaggcg ttgccgaccg gcacctgaat ctcgccaagc agctcgagcg tcaactcgcg
      541 cgcttcactg acgccggtca ggccgatgcg acagtcgccg aatccgcccg gacgctctgg
      601 cgcgccgcgg tgagcgatgt ccagaatggc ctggacgacg atcgcccgct ctactggggg
      661 cgactggcac ttcggcgatc cgtctaccgc agcgccctcc gtgaaaagga ctcgctgctt
      721 cggtccatcg agcactactc ccgaggcatg ggtgaccttc ggctcggcga tgatgtcctc
      781 aacgtgctga tcacgggatt cgacccattt cacctggaca cccggctcga tcagagcaac
      841 ccgtccgggt tggcagcgct ggcgctgaac ggatccgagt tcggttccgg cggtgggcgg
      901 gccagagtct gcgcgagcat atttcccgtc aggttcgaag atttcgacga cggcatcgtc
      961 gagcgctttt tccagcagat ccttgctgag cgcgagcccg ccatggtggt gacgctgagc
     1021 atgggccgcg acgccttcga cctggaacgt ttcccgggac ggcggcgctc cgcgcaggat
     1081 ccggacaatc acaacgtgct aaccggcgcg agcccggaca aacctttgcc cccgggcaaa
     1141 accgggtcgc acctggaggg ccccgagttc ctggagttct ctctcccggt gcatgccatg
     1201 cgggcggcgg acggccactg gccggtccgc gacaaccgat gggtcaccac catcgagcgt
     1261 ggggagttcg aggcgggatc tctggcggac ctgcaatccc agctggccgt gcgaggttct
     1321 ggagggggtt acctgtccaa cgagatcgcg taccgaagcc gtctgctcca tgggcaactc
     1381 ggctgcagct gccccatcgg ccacgtccac acccccgctc tccagggcca caacgcagcg
     1441 ctggagcgac tcatggtggg acagatccgg cagatgctgg cgcaggctgc cgccagcctg
     1501 acgccccggc cgccgtccta gctcggatcc gcgatcttca gcagctgctt gccgaggtta
     1561 cgaccggtga acagcctgtt cagggtgtcc ggaatgttct cgaagccttc ctgaacgtct
     1621 tcctgatgaa cgatctcgcc gctgttgatc caaccgagca gctcggggac cgcgatgtgc
     1681 gaacggtcca tgtaatcgat gacgatgaag ccctccatgc gcgcccgcat gatgaccagg
     1741 ttcatgaggt tcaccggccc cggctgcggc tccgtggcgt tgtacgccga tatgcccccg
     1801 cagaggacga cgcgcgcgcg catgttgatg tgattcagcg cggcctcgag gattccgccg
     1861 ccaacgttgt cgaaaaagac gtccacccgg ttcgggcaca actcgccgat ccggcggtcg
     1921 acgtcttcgt tcttgtaatc gatcacgtcg tcgaagcgcg cctcgtcctt gagccacgcg
     1981 cacttctccg ggccaccggc gatcccgacc acccgacatc ctttgatacg cgcgatctgg
     2041 ccggctaccg atcccgtcgc gcccgcggcc cccgatacga gcaccgtctc cccggccttc
     2101 ggctggccaa gatccagcat gccccaatag gcggtcagcc cggtcacccc gagcgcgccc
     2161 agggcaagac gcggtgttac cccctccggc agcttgcgca gccccatggg ccccatgccc
     2221 gccgacatca ccgtgaagtc ctgccagccc cccgtggtct gaacgagatc accggcgctg
     2281 aagtcgtcgc ttctgctctc aacgacctga cccacggacc ccgcgcgcat cacctcgccg
     2341 atctggactg gcggcaggta gctttcacga tcctccatcc atccgcgctg agtcggatcg
     2401 aaagagaggt aaagattgcg gatgagcact tcgccatctc cgggctccgg aatggtttct
     2461 tcccgatact cgaagttgtc tttggtgacc attccatacg gccgtttggc cagtacccat
     2521 tgtctgttga cgttcatcag cggtcctcca tgcgagcgct tgattaaatc atcggcaaag
     2581 gtcagagtca ccgcggtctg cgaatctgat aggggacaat gagcgacatc gcgttcctct
     2641 cggccaccga gctcgcccgc aggatccgcg accgcgagct ttccagcgaa gagctgctgc
     2701 tgcattactt gcagagggtc gaccgctaca acggcgccct caacgccatc gtggtggaca
     2761 tccgggagga ggcattgatg gcggcgcgcg ccgccgatgc ggcggtagcc cgaggcgtcg
     2821 acgtgccccc ctttcacggt gtgcccatga cggttaagga gtcatacaac gtcgccggca
     2881 cccccacgac ctggggcaac cccaaatgga ggggcaacgt cgcgttcgag gacgccgaat
     2941 cggtgaagcg tctgaaggac gccggcgtga tcatcttcgg gaagaccaac gttcctctcg
     3001 cgctggccga cttccagagc ttcaacgcca tctacggcac caccaacaac ccattcgatc
     3061 actcgcgcgc gccgggcggc tcgtcggggg ggtcggcggc ggcgctggcg gccgggctca
     3121 cgggtctcga ggccggctcc gacatcggag gttctattcg caatcccgca cacttctgcg
     3181 gggtcttcgg tcacaagccg acatggaacc tcctgtggat gcgcggacac gcaccgccag
     3241 gcgatgtgcg agcgagctca gacatctccg tcatcgggcc gctgggtcga tctgccgcag
     3301 acctggaaac cgcggttcgt accatggccg ggcccgacga catcatggcg cgggggtaca
     3361 cgctgagcct gccgaccctg aagagagggg ggctcggagg gctcaggatc gccatatggg
     3421 aggacgaccc cgtgtgtccg gtgagcgccg agacgaaggc ccgcgtgctc gccgtagcga
     3481 gcgcatgcag ggacgccggt gccgagatca tcgagggcgc gcggcccgac ttcagctcag
     3541 agcacagcca cgagacctac cagaatcttc tccagcccac catggcatca cgcatgccgg
     3601 acgaggagta cgagagtctt ctggaatatg tggccaatct cgatccgcgg gacaccagcc
     3661 ggacggcgcg ggtgcttaga gcccaggtcg ccaggttccg ggattggaaa cgcagccacg
     3721 agctgcgcga gcacctgcgc tggaagtggc acctgttctt ccaggactac gatctgatgc
     3781 tgaccccgat gatgccgacc tcggcgtttc cccacgatca ccgccccatc agccagcgca
     3841 ccatagaagt cgacgggacc agcatgccgt atatggatca gatcttctgg gccgggctga
     3901 ccggggtggc ctacctgccg tccacggtca taccgaccgg cctggacgac tcgggcctgc
     3961 ccatcggcgt gcagctggtt gggccggagt acggggacct catcaccatc ggcgtggcca
     4021 gggagctgga gaccgccggc ttccgcttcg ttgcgccacc ggcttacgcc tgacgggttg
     4081 cagccatggg cacaacggct cgcgcgcatc cgaacattgc gctgatcaag tactggggaa
     4141 aagcgccggg gcaggaaaac atcccggcca cgccctcact gtccatcacc ctggattccc
     4201 tggttaccga aacccacgtt gccaccgggc catcccggga cgagctctac ttcgacgacg
     4261 agccggtggc ggaccgcaag gtgctcgact ggctcgaggt gctgcgtcaa cgctacgaca
     4321 taccaccgct ggaaatccgc acggccaaca atttcccaac cgccgccggg ctggcgtcgt
     4381 ccgcgtccgg cttcgccgcg ctggccgtgg ctttggatgc ccaatgcggc ctgcagctgg
     4441 atgcggccac gctctcggat ctcgcccgcc gcgcgtcggg atccgccgcg cgttcgatat
     4501 tcggcggcta cgtcgccgtg caggggccgc gctgggtcgc ccagcccgtt ctcagcccgg
     4561 aagcctggcc cctgaagacg gtcatcgcgg tgacgaccac ggcccgaaaa tccgtctcgt
     4621 cctccgaggg aatggccatt tccgctgcca cctcacccta ctacccggga tgggtggaga
     4681 gcaccagggc cgacttcacc gacgccctgg ccgccgtcag aacgcgcgat tttcaggcgc
     4741 tggctgagct tgcggagttc agctgcctga aaatgcacgg cctcatgctg ggctcgcggc
     4801 ccgggctttt gtactggaag gccgccaccg tctcctgcct ccacgcggtt cgcgcgctga
     4861 gacaggacgg cgtggacgtc ttctttacgg tggatgcggg cccccaggtc aaggcgatct
     4921 gcaaaccgag cgccgagagc gacgtgagag cggccctggc agcggtgccg ggtgtggagc
     4981 agatcgtcgt cacgggtctg ggttccggcg cgcgggtcat cgatccatga tccacgcgag
     5041 cgccccgggt aaggtcgtgc tctggggcga gtacgcggtg ctcaccggcg cccccggact
     5101 ggtgctggcc gtcgatcgcc gggccagctg cacgctgacg ccggggggcg atacctggca
     5161 cttcgaaacc cggggactgc ccgagccagc cgtggatctc gacgttgccg atctgaccgg
     5221 agcgcacccg cccggcccgt cgggcggcgc gctggcatgg cacgttctgc ggcatctgga
     5281 cagcgaggtc ccggccggcg cgaccctgac gaccgacacc gccagcttcc aggacggcgg
     5341 ccacaagctt ggcatcgggt cgagcgcagc ggtttgcacc gccgtctacg gggcattctg
     5401 tgagctgctc ggaatcggcc cgagcctgaa cgacgccctc gccgtgcacc gaagcctgca
     5461 gggtggaagc ggcagcggca tcgacgtcgc cgcggcgtat ctcggcgggt cgttgcgcta
     5521 ccagctgcgc ggcggggccc ggcctccggc ggcagagccg ttcgatctgc ctgacgggtt
     5581 gatcctgcgc ttcatctgga cgggaacccc ggcacaaacc acccggcacg tcgcgcggtt
     5641 cgaggcatgg cgggcggaaa accagacgag caccctgacc gagctcgccc aggcatctgc
     5701 cgccctgttc gaaagcgggc atctcgtcga cgacctggcc gaatacgccg agcgactcta
     5761 cgcaatggac cgcgccgccg gattgggcat ctacaccgac gcgcatctgc agctgtcgca
     5821 agttgcgaaa gaatgcaaag tagtctacaa accctgcggc gccggtgggg gcgacatcgg
     5881 cgtcgcagcc agtaacaacg agtatcgggt cgagcgattc gtttcggccg ccctcgggtt
     5941 gggcttccag atcgtagaaa tggagaaaac cgaacatggc atccagctcg ccagctgaaa
     6001 gtggatcgcg gatacccaat tttttccgaa tgagcgtggc tgagcgcatc gccgcgcttc
     6061 atgcccgggg gctcctgagt tcggaagacg ttcatcggct gaccgccggt gaccacaccc
     6121 tgcgactgcc cgtggcggac aagatgatcg agaacgtcgt cggcgttttc gggctaccca
     6181 tgggcgtcgc gctcaacttt ctggtcaacg gccgcgacta cgtcgtcccc ctggtcgtcg
     6241 aagagccttc catcgtcgcc gggctttccg gggccgcacg gctggcccgg ctcggcggcg
     6301 ggttccgagc cagcagcacg gagccgatcc tcatcgggca ggtccaggtg gtcaacgtcg
     6361 acgaccccga taaggccatg gccgacctgc tcgccaatcg ggatgaaatt ctcgccctgg
     6421 ccaacagcct gcacccgaag atggtcgcgc gcggcggggg tgccctggac atcgaggtat
     6481 tccatcatca cgcgcccgaa gacgggcgcg agatggtcgt tctgcacctg ctggtggaca
     6541 cccgcgacgc catgggcgcc aacctggtca acaccatgtg cgagggtatc gcctcgatgg
     6601 tcgagacgat caccgacggc aaggttttcc tcaggatcct ttccaacctg accgaccgcg
     6661 ccgtggccac ggcctccgtg cgcataccag cgcagaacct ggaggggaaa ggattcagcg
     6721 gcgaagacgt ccgggacggc atcatcctcg ccgcggacct ggccacggtg gatccgtacc
     6781 gcgccgccac gcacaacaag ggtgtcatga acggggtgga tgccgtggcc atcgccacag
     6841 gcaacgactg gcgcgccatc gaagccgccg ctcacgcgta cgcggcccgc gatggccagt
     6901 accgctcgct gacacgctgg tacaagaacg aggacggcga cctcgtcggg gaaatccaaa
     6961 tccccatgaa ggtcgggacc gtgggcggct cgctggaaac caaccccagc gtccgcatca
     7021 atcaccggct cctgggcacc ccgggggcga cggagctcgc cggcatcatg gctgcagtag
     7081 ggctcgcgca gaactttgcc gctctgcgcg cgctgtcgac agatgggata cagcagaatc
     7141 acatgaccct gcacgcccgc agcgtggcga gcacggccaa cgttcccgag gagctgttcg
     7201 acacggtggt cgaagcgctc atcgagtcag gcgagatcaa agtctggaag gctcaagaaa
     7261 tcgtaaagaa cctgacccgg aagatcgcca tgcccgcaga cgtggagcga agctcggcgt
     7321 gcggcaaggt gatactgctg ggtgagcacg ccgtggttta cggaagaccc gccctggcgg
     7381 tgccaatccc cctggcggta gaggcctccg tgcgtcgcgg cggcggggac ggcatcaacc
     7441 tggtcatacc gcgctgggga ctggagcaga aggtccgcgc cagcaacgcc cagggactca
     7501 gcggcatctt gtatcacgtt ctcgagcagc tggggctgga ccaggagaac atgaccatcg
     7561 aggtcattcc tcacgtgccc cgcgccatgg ggctgggtgg ctcatcggcc aacgccgtgg
     7621 cgatcctgcg cgcgctggac cacaccttcc ggctcaggtt gagcgacggg cgcctcaacg
     7681 agctcgccta cgaatgcgag acgggcgctc acggcacggc ctccggaatc gacaacacca
     7741 tcgccaccta cggcgcgccg ctggtcttcg agcggggcga ggacggtccc aggttcgaag
     7801 agatccgcgt ggccgagccg ctgccgctgg tcatcggcat caccggaaag gagtgcctca
     7861 ccgcaaacac cgtcgccctg gtcaggcggg cgtgggaagc ccaccaggcg cgctacgacg
     7921 ggatcttcga ccagatcgcc acgctgaccc gcgccggcgt agacgccatg cgggacgctc
     7981 atttcgccga gctcggcgag ctgatgaacc tttgccaggg ctatctgaac gccctgcagc
     8041 tttcgacccc cgagctggag gagctgatcc atacggcccg cgccaatggc gcgttgggag
     8101 ccaagctgac gggaggtggt ggcggcgggt ccatggtcgc cctgtgcccg gatagccagg
     8161 atcaggtcgc gcgcgccatg gaggctgccg gctaccaggc gctcaaggtg acgattcagc
     8221 cgtagttcac tcgaccgcca tactcaggtg tgagacgtcc ggcgaagtga cgtcggcgtc
     8281 gtccgacgga tcgccgatgt ccgcaccgac ctcggcaacg tcgaactcga tggcggccac
     8341 atcgaccacg ggcgcggtgt ccaccgcaag attcggtatc aggcttccag gctcggcaag
     8401 gtcgaaatcc gctttgatgc cgggatgctc acgggaaatg cgctctgcca ggtcgacgcc
     8461 gagatctgcc acggagtaat ccggggcgac gatttccggc gcggccgaat cgggcacctc
     8521 gggcgcccag tcgagcaccg ccaggtggct gagatcgatc tcgagctcct cgaacgtgga
     8581 gcgttcgtcg gcacgcagaa cgtcgctgcc gctcggcatg acctccagcg cggacgcacc
     8641 ggagcttgcg cccgcatccg tcgccgtggt ttcttcggcc gccggtgctt ccgaggccag
     8701 cggcatcacg cgcaatctgg cgccggcctg tttgaacagg tcctgatagc gtgctgcagt
     8761 agccgcgtcc gcgcgcttct tgaccaccac cgggtgaccg ctgaagagct tctccgcctg
     8821 cgcgtcgttc agctggagcg cctgcaccag gcgtttcctg gccaccgcgg ggtgctgtcc
     8881 ttccaggaac tcgccgcgga acaccaatcg gtaagcttcg tccatcatga gcattctaga
     8941 actgtgcgcc acatctactt cggcgcaatg accgcatgtt cacacttctc gcaagcggaa
     9001 aatgggcacg ggcgcacaag tcgcgccagt gattggtaaa agcgtcccag atatcggcgt
     9061 tgagccgtgg gcacccgtgc gtttgttact cgacgtccct catggcgctg gttaaggtat
     9121 ctcgaacggc gcaagcgggc gccgcgcgcg caaaggcaca ttggactcca gatgagcatc
     9181 aacatcggga aattcgagta tccagcgcac cccaagctga cagcgcaccc cacgctgtgg
     9241 aaagtgctgc cagacgcaag ggagctggtc accgcgaacg tcgagcggct ctccgagctg
     9301 caggagcgac tgtacgccaa caaccgctat tcggttctca tgatcttcca gggcatggac
     9361 gcagcgggga aggacagcac catcaagtac gtcaccagcg gcgtcaatcc catgggcttt
     9421 caagtggtca acttcaagca gcccacctac aaggaactcg accacaacta cctgtggcgc
     9481 tactggcgcg ccatgccgga gcgaggccgc atcggcatct tcaaccgttc gcactacgaa
     9541 gaggtcggag tcgcccgcgt tcaccccgag atcatcgagc ggcagaatct gcccagcgag
     9601 catgtcaacg gcgatttctg ggagaaccgc ttcgaggata tccgtgccat ggaacggcac
     9661 ctgcacacca acggcaccat cgtattgaag ttctttctga acgtttccaa gaaagagcag
     9721 aaagcgcgcc tgatgaagcg cctgacgaac cgcgacaagc actggaagtt cgacccccgc
     9781 gacatcgacg agcgcgtgca ctgggacgac cacatgaaag cctacgaggc cgccatcgcg
     9841 gccacccact tcgatcacgc gccctggtac gtgattccgg cggacgacaa atggaccatg
     9901 cgcgtcatcg tcgccgaggt gctctgcacg cacctcgagc agctcaggct cagctacccg
     9961 ccggccaagg gcgcgcacaa gcacgcgctg gccgaggcgc tatcacgtct tgaagccgag
    10021 aaggagtgag cctagccgcg gcccatgacg gcaagcgccg cgcggttcaa ggacacccca
    10081 cgatcatcgc ccagaaggct cggttccatg cgattcatca cgtaagcgat gcacatgcgc
    10141 gactgaggag cgacgaccac cgaggacccg ccccagccgc cccagaacgc gagttccctt
    10201 ggagattgtc cgtgtaacag ggcatatccc ataccgaacg tgagcggcgc tttgagaacg
    10261 aggtcgcggc cgtgggtctg ctcgtcgaaa accgcccggc acccagcctc ggacaggatc
    10321 cgagttccag cagacacgcc gccattggcg atcaccgtct ggatgcgggc cacggatcgc
    10381 gcgttgccgt gaccgttggc ggccgggatc tccgcttcgc gccattccgc ggtcgccgca
    10441 tcttccgccc gcacgaacgg gctgcggaac gcgcgcaccg cgatgctggc cgctcggccc
    10501 tcgcctccac ctctccctga gggctcatgc gcaagagccc cgccgaggct cacacccggc
    10561 ggcagcaggt cgccaatgcg cgagaagtgt tcgggagcgg tgccgatgtg aaagtcggcg
    10621 cccagaggct cagctatctc gcgccggaag aacgtgccgc aggacatacc cgtcacgcgg
    10681 cgcacgacct cgccgatgag gtgtccctga gtgatcgcgt ggtatccgct cgccgttccg
    10741 ggttcccacc agggcgcctg cctttcaagc gcgttgacga cccgctccca gtcgtagaga
    10801 gcttcaccac gaatcggctc atcggtgccg gcaaggccgg cggagtggct catgaagtgt
    10861 cttacctcga tcttctcttt gccgttctgg ccgaacgccg gccagtaccg ggtgaccggg
    10921 gcgtaaagat cgatctcgcc gcgatcggcg agcaccaggg cgcaaagcgc tgacatggtc
    10981 ttggtggtcg accacacgtt gacgatggtg tcctgctccc agggcagaga ctttgcggca
    11041 tcccggtggc cggcccacat gtcgacgacc atctcgccct caaccgtcag ggcgagcgaa
    11101 gccccaacct cgtccttgtc ggcaaagttt cgggcaaaga cctcacgcac gggcgagaag
    11161 cgctcgtcac agtagccgtg gatttcgatc atgctgaact ccgagttcaa gggcagaatt
    11221 agtcggccag aagcctccac tgcagcagcg tgaagggttc gtccacgtcc tcgtgatgct
    11281 cgaacacggc ctccacggcc gagccgatct ccacatcctg gagcgggtcg ccgagcaggt
    11341 tgccgaccaa tcgcaccccg tccgcctggg gaatctcgac cagaacgacc aggtaagggc
    11401 cctgctcggc aagcgcggga tggaccgggt gccagaccct ttcgtagctg tagacgatac
    11461 cgcgtggtgt taccgcttcg aaagcaagct caaagccgag acagtggtgg cagatccact
    11521 cgggacccca ctgccagccg ccgcactgcg cgcaccgctg cagcatcagc cgatcctcgc
    11581 gcagcccgtc ccagtaagat cggtccaggc cgtccggaag cggcgcgggc tgtggcaggc
    11641 cgggtctgag gtagtagctc atagcgccgc ctccgaaccc aggatgcacg cgctcaccgg
    11701 ggtgaccatg gggcccgaga tcaccatgga tatctctgcg cccatcacct gattggtgga
    11761 ctcaccgcga atctgcctaa ccgcttcgat gttgagcccc agaccgtgca tgtagcactc
    11821 ggcgagattt ccgccgctgg tgttcagcgg aaggtcccca tcgggcgccg tcaaccgctc
    11881 cgggacgaag aactcgttgc accgttgcgc ctcgcagaat ccgtgctcga cgatgctcat
    11941 gagcacgccg ccagtgaagt tctcgtagct ctgcagcacg tcgacgtcgg cggggaccac
    12001 cccggccatc tcgtacaggc ggggagccag cgtcttgaac gtgctcgtcg cgtagtcggg
    12061 cgtgttgtgc accgacgccc cggcccgata gtgggaaccg gacacggcac caagcagata
    12121 ggcaggaacc gggcggcagt cgcctgcacg atccgccggc accacgatca tggcagccgc
    12181 gccgtcgttc tcgagacagc aatcgtagag atgaaacggc tcgacgatcc atcgagacgc
    12241 atcgtaagtc gcctcatcca gaggccgtcc gtgcatgacc gcacgggggt tgttctgagc
    12301 atggtggtaa gacgccaagg agatcgcccg cagcgccgac tgctcaacac cgtggtcgtg
    12361 catgaatcgc tgcaccttca tcgcgaacat ctgagctggc gacatcaggc cgtacggcac
    12421 cacgtgggcc atgtcaccgg cgatgacgtt gcgtcgcggg ccctgcccga accggccgaa
    12481 ctgcccctgg gcgagcgcgc gaaaaaccac cacgcaatcg gccatgccag tggcaacggc
    12541 ggccgcggcg ttgccgagcg ccccggagcc gccccctccg ccaccgcccc actgcatgtt
    12601 ggcaaagcgg agttcacgac aacccagcgc tgcggccagc cgcgacgggt cgctgcggtc
    12661 gttgctgtag gaagcgaaac catcgacgtc cttcgggtcg atgcctgcat cctcgcaggc
    12721 cgcgaggatc gccttgagcg cgagcttgaa ctcggcatcc ggcgattggc cggccttgta
    12781 gtacatggtc tcgcccacgc cgacgacggc gacccggccc ctgagcgagc gattcatctg
    12841 gtttccccca tcccggggaa tctacaccga atcaccgcca tgcgacacca cggaaccgac
    12901 cgctgccacc ggctcggcaa acgtctgggt tcggtagtag aacacctctg tggcatccga
    12961 ccagtggcca gccgggaagc cggctttggc cttgagctcg tccaggaaaa ctcgtggatc
    13021 cggcaggctt tcccagacct tgggcagaaa ggtaccgcgc cggccgcctt cctcgatcac
    13081 cagacccatg gacggctcga gacgctccag gagctccccc tcgctggcta cccgcatccg
    13141 ctccaggggg gagagaaccg aaagctcgat gcgcagacgg ttcagctcgc ctgcctgaac
    13201 cggagggaag cgcgggtcgc gaaatgcgga gtggcaggcg ttttccacca catcgagcgc
    13261 gagcggcctc aacgcctcca gcgaacccgt gcaaccgcgc aacgacccgg cttgggtcag
    13321 ggtgacgaac gtcgctcgag gctcgagaag ctcgggaagc aggctgggaa cgtccaccac
    13381 ggacatcggg tccgcgacgc catggcgttc gagaccaatc cgaatggcgt cccgcgcgat
    13441 gcccagcagg gcctgtcgcg cgtcggggcc gtaatcagtc gagggcaaac gcgccataac
    13501 ccaccacccg atcacggtcg ccggccgtat cgcccgaatt gcgaaggtcg agctcccgca
    13561 cctgaaggtc gtgaccggta gccgccagca tcaaaccgtt gatggcgacc gctccgcacg
    13621 cctgctcgcc ggtcaggttg caggcgcgcg cgagaatctc gcctgccgtc gccgcatcga
    13681 gcgcctgagc cacctcgtag ggatgatagt gggacagatc ggaactcacg acgatgaggg
    13741 tttcgtctcc gccccagagc aggtcgagca cgtcggccac cagctcgctc gagcacgcgc
    13801 cgacgaccac gggaacgatg gaaaaacgat caagcaccgc ctggaggaag ggcaggtgca
    13861 cctccaggca gtgctcggcg gcgtgggcct ggtcagaggc cagcactccg gggagctcga
    13921 gcagtcgggc cacggcttcg acgtccaccg gcacgttacc cgtcgggacc gcaaacgcat
    13981 cagtctcggg gaccgccagg ccttccagat acacccgatg ggccgggccc atgaggatca
    14041 cacgttccac ctcgtgccga aaaggctcga gggtccgata cgccgtcgcc gccaccggcc
    14101 cggagtatgc gtagccggcg tgaggcacga tcagcgcctt cggtcgcagg gcgaccgtgg
    14161 gcgcatccgc gcggtccagg tacgcccgca ccgtcccggc cagctcggcc ggatctccgg
    14221 gatagaacag gtccgctacg gcgggcgatc tgctgaccat ggcattcgga agattgttaa
    14281 ggcgcttggt atatgggggt accgacccca taaagcaacc atggatcaga caatcgtgcc
    14341 cacgcgctac tggcatacgc tggacgatgg ccgcactcag tgcgacgttt gtccgcgcca
    14401 ctgccgcctg cgcgagggcc agcggggact gtgctacgtg cgccagtgtc tcgacggtga
    14461 ggtgaagctc gtcagctacg gcagatcgtc ggggttctgc gtggacccca tcgagaagaa
    14521 accgctgaac cacttctacc ccggcagcag cgtgctctcc ttcggcaccg cgggctgcaa
    14581 cctcgcctgc aagttctgcc agaactggga catgagcaag tcccgcgaga tggacaccct
    14641 ggccgatcga gccatgccgg agcggctggc cgaagccgcg gtggatctgg cgtgccgaag
    14701 cgtggctttc acctacaacg acccggtcat cttcctggag tacgccgtcg acgtcgccga
    14761 cgcctgtcgc gaagtcggcg tgaagtccgt ggccgtgacg gccggatacg tggaccccga
    14821 gccccggcgc gagtttttct ccgcggtgga cgccgccaac gtcgacctca aggcgttcag
    14881 cgagcgcttc taccacaaga tctgcggcgg ccacctcgcg ccggttctgg agacgctgga
    14941 attcatccgc cacgagacca acacctggct cgagctgacc acgctgttga taccgggcga
    15001 gaacgactcc gacgaggagc tcgacgagat gacgggctgg gtcgtcgagc acttgggtcc
    15061 cgacgtgccc atgcacttca ccgcctttca cccggactgg aagatgcgcg acgtgcccag
    15121 cacgccccca ggcaccctga cccgggctcg cagcattgcc aggcgcaacg gggtgcactt
    15181 cgcctacacc ggcaacgtgc acgatcctgc cggctccagc acctactgct cggcctgcga
    15241 tgccctgctc atcgagcgcg actggtatca gctgggccgc tggggactgg acggggccgg
    15301 acgctgcagc gcctgcggcg agccgctggc cggacatctc gaagggcctc cgggccagtt
    15361 cggaagccgg cgcatcccgg tgcgactcga tcaatgagca taatttcagc gcaatcaact
    15421 gatgacaaag ccgctgacta tggtagggtt cgcttccgag aaaaacctgg ctgagtgagg
    15481 cccccaagtg aaaaatcttt tctttgccat gatcgccacc ctgctgctcg caggctgcgc
    15541 aactaccgaa caggtcgagg ctctccagaa ccgcgtcagc gcgctggaga accagattgg
    15601 ctccgctaac gacgctgccg gagatgccgc ttctgctgct tcccgagcgg cgagcgacgc
    15661 cgcggctgct cagtccaccg cggagcgtgc gctggacgcc gccaatgcgg ccaacgagcg
    15721 ggcggagcgg atttcggaga cctgctgctc ccggaagtag tgctccgtta aagcctgacc
    15781 ggaatcccgg tcagtgctgc actgacctcg tcgacgcgac gccagtccac actggcgtcg
    15841 cgtttttctg tggcatcgac aatcagcgca acgagttcgg aagcctggtt ttcgccatat
    15901 aggttgcggt gcgcctctat ccagagctcg ccatcgcgcc atcccacctt gtagggctca
    15961 ttgacgatgt atacgggcgt cccgttcgga acgagctccg acagcgtctc tatgtggccc
    16021 ggatacaggc ggacgcagcc gtggctgaca ccctgaccta cgccgaacgg tttgttggtg
    16081 ccgtgaatga aatatccagg cgcgctcagc tgaatggcta gatgccccag agggttgtca
    16141 ggccctggcg gcaccacgtg gggaagcgga tcacccatct ctgcatgctc ccgccgggcc
    16201 gcatctgttg gggtccagct agggttctct atcctggtga ccaccttggt gctgatgagc
    16261 ggcgtgggga agtccatccg ccctaccccc accgggaacg tgatgaccga gccgcgacca
    16321 ggcggatagt agtagagccg atactcggcg acgtttatga cgatcccttc ccttggcgcg
    16381 gagggcagca cgtaggcggt gggcatgacg atacgggcac catctccggg tagccaggga
    16441 tccaccccgg ggttggcatc caccagttcc tgatagccca cgccgaattg ctgagccaca
    16501 cctgcaaagg tgtcttcgta ggcgagcgtc aggctgatgc tggcgccgat gacgtcctct
    16561 ccgccttcgg gcagccgata ggacgccgct tcggtcgcgg ccgccgcaac aagcaggcag
    16621 gccagcaggg ctgcggcaga agtccgtgat gtcgggacag acaagtcgtt ctcaaaccat
    16681 cggccgggag gacgccattg tccccatctg gattcccagg tccagtccgc tgccgcggct
    16741 gcattcgcgt tgatcgcaat ggggcccacg gctatggtag cgggcatcca tgtaagcggg
    16801 aggatcttcg catgccaatc aacccagatg ccgtcggcgc cgagggacgt ccggtgcgcc
    16861 gcagctggtc gtccaaagat gctttgctct acgctgtggg cgtcggttca ggcaccgatg
    16921 agctcgcttt caccaccgag aacacaaagg acgtggcgca aagggttctg cccaccttcg
    16981 ccgtgatcat cggcggcggc ggtgtgccga tggacgccat cgggtccttc aaccccgcgc
    17041 tcatggtcca tggcgagcag ggcatcgagc tctacgacga gatcccgccg gacggagaga
    17101 tcgatagcgt gggtaaagtg accgccatct gggacaaagg ctccgcggcg gtgctggagt
    17161 tcgaatccac atccacgaac gtcgccacgg gcaagccgct tctgaagaca cgaacgagcc
    17221 tcttctgccg cggcgaaggg ggctggggcg gcgatcgagg gcccagcgag aaagtggaat
    17281 ttcccgaccg cgagccggac catcaggtca cctacgcgac cagggaggat caggcgctca
    17341 cctaccggct ctcaggcgat cgcaacccgc tgcattcgga tccttccttt gcggcgctcg
    17401 gcggcttcga gaagcccata ctgcacgggc tgtgcacgtg gggattcaca ggtcgcgccc
    17461 tcctgcacac cctttgcgac ggagacccga gccgattcaa gagcatgaac agccggtttt
    17521 ccaagcccgt gatccccggg gacgagttga cgatctcgat atgggtggtg gacgaaggca
    17581 gggctttgtt caggacgacc aatcagaacg gcgacgcggt catcgatcag ggcgtgttca
    17641 catacgcctg atagcgtgcg cctgcggcga gcggcaatga tcgagcttgt tctgcggctg
    17701 tcgctctgga taagcctgcc gctcaacgcc ggtgctgcct acatcctggc tttcccctcg
    17761 tcctggccgg gtcagctgct gggcctgccg gcagcgcccg atcccgtgta cgcctacctt
    17821 gcagcatatc tggtggccct gctcgggctc gcctacggct ggatggccct gcaaccgagc
    17881 atcgtcaggc ccctgctgtg gttgggcgcc cttggcaaag ggggcgcctt cgcggtggcg
    17941 gcagcccttt ggcttgccgg tctggctcgc gctcccctgg cggccgccgt gaccgtcgac
    18001 ctgctgctcg cggcgttctg gatcgcctgg ctcacaggca cgcggggaaa ggcgagcgct
    18061 gacggttgat cgcttgattt ccgggtcagg cgtagccagc gacgccgggc gtcggggccc
    18121 ggtcagggaa tgggatgcgc gtccaggccg agagcgcttt cgaacgcgct gccggcacga
    18181 acgagggtag gctctcccag gggtcgcgcg atgagctgaa aggctctcgg caggctgccc
    18241 cgttcgagct tcgccggcag ggtgatggtc gggtgaccgg aataattgaa aggcgcggta
    18301 aaggtgatga gatccgcccg atctgacggc cgtcgtggtc cgtccgccaa tgcgtcggct
    18361 gggggcggca actcgggcat gcagggagcg atgatgacgt cggcgctttc caggcacccg
    18421 ttgagcccat cgcgaaactc cgcgcggacg gtttcgagca atccgtaatc ctcccgggaa
    18481 accgcccgcc cgtaatcgat gaaggccgcc agcacgggtc cgtattccgc cttgcggtcg
    18541 ggatagaaat cccggtgggc acgcgcgcac tccataccgc aggtgactcc ccaccctcgc
    18601 acgagcgtcg cggcactcgc cggcacctcg atatcgacca ccgtggcgcc gagctcccga
    18661 aagatctcca gggcttcccg gatcgtctcc accacgggct cgtcgacgcc tttggacacg
    18721 tagtcccagt cgacgccaat tcgaaggccc ggcagggtat cggccacaga ggcagcgtag
    18781 ttgggcactg ggtcgggctg cgtggtgacg tcgtacggat cggcgcccgc gagcacctga
    18841 agcagccggg cggcgtcctc aacgctgcgc gccatggggc ccacgtgatc cagcgactcg
    18901 gctaggggaa acgcaccgta tcggcttacc cttccgtacg taggcttgat gccgacgagc
    18961 ccgcagcagg cgctcggaaa acggatgctg ccgcccgtgt ccgagccgag cgcgccaaag
    19021 gacagccctg ccgccaccgc caccccggat cccgaagacg atacgccggg ccagtagtcg
    19081 ccgtcccagg ggttcttcgg cgggtccacg tccgggtgat gggtcccgta ggcaccctcc
    19141 gtgagctgcg tcttgccgac gatcaccgca cctgcatcct tgagccggcg caccaccgtg
    19201 gcatcctcgt ccggaacccg ctcgcccatg acccgagtgc cgctggcggt taccacgccg
    19261 cgggtatcga acaggtcctt caccgcgacg ggaacgccgt ggagggcgcc cagcggcttg
    19321 ccgtccgcac gatcctgatc gaggcgggca gcaaccgcgc gggcctcgtc ggccatgacc
    19381 gttgcaaagc tgttcaggtt cgagtcgagg gcttcgatac gggccagtat cgcctcggtg
    19441 accgccgacg cgctgagctc gccgctcttc atccggcgac acacaccggc cagcgacagg
    19501 tagtgaaggt cggtcatgca gccgcttcgt tcaccgatgc gctgtcgaag atcttgccgg
    19561 gattgaggat gcccttggga tcgagggccc gcttgatcgt cctcatcagc tcgatttcct
    19621 gctcgctgcg ggaaatgccg agatagggct tcttctcgag accaacgcca tgctccgcgg
    19681 agaccgagcc ctgaatcgca gcaagcggct cgtagacgca cgcctccact gccctgcgag
    19741 tctcgggacc tccctccccg acggagatga ccaggtgcag gtttccgtct cccatgtgac
    19801 cgaaggtgaa attgttgacg tcctgataac gcgcttccag gcgggcattg acctccgcga
    19861 ggtatgactc catgtgggag atgcgcaggc tcacgtcgaa agtgtagatc ggggcgtaac
    19921 gggccgtgtg ctccacgctg tctcgcaacg cccacagcgc caggcgctga gcttccgact
    19981 gcgcgatgac cgcatccgta atgagagatt tttcgtaagc ctcggcgagg gcggcttcca
    20041 ccgcagcgtc atcgctgccc atggcctcga ccagcgcgta gtagggataa tcccggggaa
    20101 tcggcgcctg gttctccgcg ggcggcgtgg tgaccagggt atagaagttg ttccacatga
    20161 cctcgaaggc ggaaagcgcg cctcccagcg ctgcgtccat gtgcttgagg aactgcgcca
    20221 gcttggcgaa ctcgtccagc gagacgagca gggtcgcacg gcaggcaggc tgctcacgca
    20281 gccgcaacac tgcccgggtg accaccccga ggcttccttc cgtgccgatg aaaagctgct
    20341 tgaggtcgta gccggcgttg ttcttgatca tctggttcat gctggagagc accgtgccgt
    20401 cagcgagcac cgcttccagg cccagaacca tgtcccgcgt catgccgtag cgaatgaccc
    20461 gattccctcc ggcgttggta gatatgttgc cgccgatggt gcagctgccg cgcccgccca
    20521 gatcgagcgg aaacatgagc tggtgctcct cggcagcttc ctgaagcacc tgcagaatta
    20581 ccccgctctg aaccaccatg gttcgatcta ccggattgat ttcctcgatc cggttcatgc
    20641 gttccagcga gagagcgacg tcgtccgggc cggtaatggc gctttccacc agcccggtca
    20701 gcccgccatg gggaacgatg ctctggccgt gggcattgca gatggccagg gtgcggctga
    20761 cttccgcagt ggtcctgggc cggacgagcg ctttggcctg gatcgtatcc gagcgccaga
    20821 tgccagcctg gcggctgtgc acgtcttccc cggtcaaaac gcagtcttcc cccaaagctt
    20881 cgatgagttc ggctacgacg acgtcagtca tggcttgatg tcctggaatg cggcggaagc
    20941 cgagcttacc tcagaagcga acgtcctgcc tcaaggccac ccatccccgc tggccgggag
    21001 ggtgcagcat tgtttacctt tggttctcgc ccgaccctca acgagcacgt catggccaga
    21061 accttcaaca aagcctacta cgaccgctac taccgcgacc cgcggacccg cgtgaccacc
    21121 ccggcgggca cccggcgcca ggccgggttc atcgccgctt atctcgacta tctggagctc
    21181 cggccgcgca gcatcgtgga cctcggctgc ggaacgggca ccctgctgcg cgcgctgggc
    21241 aagcactttc cacgagcgag gctgacgggg gtggaggtga gcgactacct gtgccgtcgc
    21301 cacgggtggc tgcagggatc ggtagcggat ttccaggccg agcatccttt cgatctcgtc
    21361 gtctgcaacg acgtcctcgc ctatctcgac gatgcgggct gcaaccgggc cctgcgcaac
    21421 ctcgcctcgc tgaccggtgc cgcgctcttt ctcggcgtgc tcaccgagga ggacctgaaa
    21481 ctgtgtgatc gccgccgcac ggacccgtcc cagcacgcgc ggcccgctgc ctggtaccga
    21541 cgacggctga acagacactg ggcgaacgtg ggcggcgggc tctatctcaa gcgcccccta
    21601 ggcgtaaccg tctggaccct cgatgcgctg aacaccgtcc cctcctagag acgtcgaagg
    21661 ttgtcgtcgg gattgcggtc gatcatcttg ttgtaaggat cgatgcctac ccgctcgggg
    21721 cgctcgtcca cgcgtaccgt gaacgtctgc tctcccgata ccacctggcg cctctcggcg
    21781 aggagcggat ccggcagatc atcctcgctc agatcttcgg gcgcttccgg aaagacggcc
    21841 agatcgaaca tctggctgag cggcacctcc gcctccctgc cctcgccgtc cgcgtcgagc
    21901 ttgcgggccg caaccgtgat cgtcacatcg aaacccccgt cgttctcagc caccgatgcg
    21961 tccgtcaccg cgaggtcgta taccgtgatc ctctcgaaca gatccgtgat gagcggctgg
    22021 tgctccgccc cggccacggc ccggaactcg tccagcagat ccctggaggt tggaaacaag
    22081 ccttccttga acccgtattt cgcgatgaag ctcttgagcg cggcg
//
LOCUS       DBNHKV010000126         6101 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_868132, whole genome shotgun sequence.
ACCESSION   DBNHKV010000126 DBNHKV010000000
VERSION     DBNHKV010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_868132"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="AC16PP_03955"
     CDS             <1..294
                     /locus_tag="AC16PP_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003727 - single-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0043023 - ribosomal large subunit binding
                     [Evidence IEA];
                     GO_process: GO:0034605 - cellular response to heat
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp15 family protein"
                     /protein_id="HIR6480809.1"
                     /translation="GATVRPKVSREVSVGDVVTVRRGGAPETVVIRAIAHQRGSASVA
                     ATLYDETPASIEGREAQRAQRQLERLGLKVPSTKPGKRDRRALKKLKEDSAPS"
     gene            291..1187
                     /gene="hslO"
                     /locus_tag="AC16PP_03960"
     CDS             291..1187
                     /gene="hslO"
                     /locus_tag="AC16PP_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIR6480810.1"
                     /translation="MTADRLHRFSFQNAPIRGNWVRLDAVLAEVFARQSYPGRLQAVL
                     AEALSAVAMLADTVKFQGTISLQSRGRGPVTTLLAECRSRSVLRGIARWDRSAGIPED
                     APLGELLGDARLAISLVDSDPATPRPVSYQGLVTLDHESLAGNLEGYFAFSEQLPTRF
                     YFGRAGHTVTGLLLQRLPADDDATEIDLDQHESDWKQIQEAAGESSADLAALPPQALL
                     RRLFPSRPLTLQPGRPLSFGCTCSRDRSTASLNAVPAPELLDILHSDGEVTVTCEICG
                     TVYAYDPIDIHLLLQREAPQLH"
     gene            1281..2846
                     /locus_tag="AC16PP_03965"
     CDS             1281..2846
                     /locus_tag="AC16PP_03965"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HIR6480811.1"
                     /translation="MAVDIESTAKHTTHLNLAAAALAELAVLRGEGQFASNGAIVVRT
                     GHRTGRSPRDRFIVEEPSTADAIDWGTINQPIAQDVFDALWDRVQIHLEDRERFVSNL
                     HVGADPEHYLPIEVTTEYAWHALFGRALFIKPENYNPHNKSVWNIANAPSFVCDPERD
                     GTASDGTVMINFAQRKVLLAGMRYAGEMKKSMFSVLNFLLPEKDVLPMHCSANMGHDG
                     AVTLFFGLSGTGKTTLSADPDRLLIGDDEHGWGKGTVFNFEGGCYAKCIDLTRENEPL
                     IYDAIRFGAIVENVIIDQDTREPDYTDGSLTENTRACYPRSNIEAKVTDNRGGEPGQV
                     VFLTCDVSGVLPPVSLLSKEAAAYHFLSGYTAQVGSTEVGSTEAYKATFSTCFGAPFF
                     PRAAGVYADLLIKRIEEFGSKVFLVNTGWTGGGYGVGKRFAIPDTRAIIRAIQSGALD
                     DAETRRLPGFNLDIPTSVPGVDAGLLNPRDTWSDKAAYDESARSLIAKFAENFEKFSV
                     AQAIVDAGPTTDY"
     gene            2886..4457
                     /gene="gshA"
                     /locus_tag="AC16PP_03970"
     CDS             2886..4457
                     /gene="gshA"
                     /locus_tag="AC16PP_03970"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003120398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HIR6480812.1"
                     /translation="MAHAAAPIDPALLDALRDQPASLREIQRGIEKESLRVTPEGSLS
                     DRPHPAALGSALTHPNITTDFSEAQLELITEVHQSPEATLAQLEGVHRFVYQNLDGEL
                     LWSASMPCVLGTDEEIPVGRYGGSNIARAKTIYRLGLGNRYGRLMQTISGIHYNFSVP
                     QTFWKVLAESRGKEPTQEFVTEAYFGLIRNFRRFSWLLIYLFGSSPAICKSFVKNQPH
                     ELASFDEGSLYLPHATSLRMGRLGYQSDAQRSLHISYNGLEQYAASMHTALTRPYPPY
                     EAIGVKVDGEYRQLNASLLQIENEFYGTIRPKRRIVSGERPLNALRSRGVEYVEVRCL
                     DLNPFLHMGIDTVEIRFIDAFLLYCLLADSPEDSERESELMGKNQLAVVERGREPDLQ
                     LEQLRGTITRKAWAEDVLHACRPLAELLDEATGSELYRHALSEQSSKNRQPALTPSAR
                     VLAVMQTQGIPFFRFAMNQSIAHKGYFDEHPMRGEQLAEHQATSQQSLARQQEIEAAD
                     SVDFDVFLKDYLALE"
     gene            4454..5095
                     /locus_tag="AC16PP_03975"
     CDS             4454..5095
                     /locus_tag="AC16PP_03975"
                     /EC_number="3.1.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF016239.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HIR6480813.1"
                     /translation="MNFLAHCLIAAQSTDPEEPDLVAGGFLGDFVKGPIGPELPPGLA
                     LGVRLHRRIDAFSNRHPAIQGSCNRFAGPLRRLAPVLVDIIADHCLAKHWEQFSNEPI
                     AAFTARTYDRIASHERWLPDHGHRFLSYMRDEDLLAGYARFEAMHRALCSVTRRLDRE
                     HLNADMLSATEANLPGLEADFLGYFPELVAHARQWLRDEGPPPGPQTPGTVQR"
     gene            5168..>6101
                     /locus_tag="AC16PP_03980"
     CDS             5168..>6101
                     /locus_tag="AC16PP_03980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HIR6480814.1"
                     /translation="MKAVLCKAFGPPEDLVMEEVANPEPGPGQILVDIHASALNFPDV
                     LMIAGKYQSLPPFPFSPGGEIAGTVAAVGDGATRFKVGDRVFGGTGHGGFAEQIALPE
                     MAMRPMPDEMSFVEASAISTTYGTSYYALKQRADLKPGETLLVTGAGGGVGLAAVELG
                     KAMGARVIAAASSAEKLAAARSAGADELIDYSDGELKEKVKALTDGKGADVIYEPVGG
                     DLFEQCMRCVNWYGRILVIGFAAGDIPRVPINLILLKSCQVVGVFYGAWSARCRDEAD
                     ANFREILDLYRDGKIKPMIGRTYPLEEYAAALRCL"
BASE COUNT         1139 a         2034 c         1923 g         1005 t
ORIGIN      
        1 ggggcgacgg tccggccgaa ggtcagcagg gaagtgagcg tcggagacgt ggtcacggtg
       61 aggcgagggg gagcgcccga gaccgtggtg atccgggcga tcgcccacca gcggggcagc
      121 gcctccgtcg ccgcaaccct ctacgacgaa acgccggcaa gcatcgaggg gcgagaagcc
      181 cagcgcgcgc aacgccagct ggaacggctc gggctcaagg tgccgagcac caagccgggc
      241 aaacgcgatc gtcgagcgct caagaagctc aaggaagatt cggcgccgtc atgaccgcgg
      301 accgcctgca tcgtttcagc tttcagaacg cgccgattcg aggcaactgg gtgcgtctgg
      361 acgccgtgct ggccgaggtg ttcgcacgtc agtcctatcc gggccggttg caggcggttc
      421 tggccgaagc cctgagcgcg gtcgccatgc tcgccgacac cgtcaagttc caggggacca
      481 tcagcctgca gtcccgaggg cggggaccag ttacaaccct gctggcagag tgccgctcgc
      541 gaagcgtgct gcgcgggatc gctcgatggg accgatcggc cggcattccg gaagacgcgc
      601 ccctgggtga gctcctcggt gacgctcggc tggccatctc tctcgtggac tcggatccgg
      661 ccacaccccg tccggtgagc tatcagggtc tggtcaccct ggatcacgaa agcctggccg
      721 gcaacctgga aggctacttt gccttcagcg agcagctgcc cacacggttc tacttcggca
      781 gggcgggaca caccgtcacc gggctgctgc tgcagcggct gcccgccgac gacgacgcca
      841 cggagatcga tctcgaccag cacgaatcgg attggaaaca gatccaggaa gccgcaggcg
      901 aatcctccgc cgacctggcc gcgttgcccc cgcaagcgct gttgaggcgt ttgtttccca
      961 gccggccgct gacgctgcaa ccgggacggc cgctgtcttt cggctgcacc tgcagccgcg
     1021 atcgcagcac cgcctcactg aacgcggtgc cggccccgga gctgctggac attctgcaca
     1081 gcgacggcga ggtcacggtg acctgcgaga tctgcggcac cgtctacgcc tacgacccca
     1141 tcgacattca cctgctgctg caaagggaag ccccgcagct tcactgagcg agcttccctt
     1201 acaatccctt tcattccgaa taggccaaat ctcaccgctc tggcataatc gatcgattgt
     1261 caggggaaga cgctgaaagc atggcagtcg acatagagtc aacggcaaag cacaccactc
     1321 atctcaacct tgccgccgcc gcgcttgccg aactggccgt attgaggggg gagggtcaat
     1381 tcgccagcaa cggcgccatc gtcgttcgaa ccggtcatcg caccgggcgc tcgccgaggg
     1441 atcggttcat cgtggaggag ccgtcgacgg ccgacgccat cgactgggga accataaacc
     1501 agcccatcgc gcaggacgta ttcgacgcgc tctgggaccg ggtgcagatt cacctggagg
     1561 accgcgaacg gttcgtttcc aacctccacg tcggggcgga cccggaacac tatctgccca
     1621 tcgaggtgac tacggagtac gcgtggcacg cgctgttcgg gcgggcactg ttcatcaagc
     1681 cggagaacta caacccccac aacaagtccg tgtggaacat cgccaacgca ccgagtttcg
     1741 tctgtgaccc cgagcgcgac gggacggcca gcgacgggac ggtgatgatc aactttgctc
     1801 agcgcaaggt gctgctcgcc gggatgcgct acgcgggcga aatgaaaaaa tccatgttct
     1861 cggtgctcaa cttcctcctc ccggagaagg acgtcctgcc catgcactgc tcggcaaaca
     1921 tgggccacga cggtgcggtg acgctgttct tcggcctgtc cggcaccggc aagacgaccc
     1981 tgtcggcgga tcccgaccgg ctgctcatcg gcgacgacga gcacgggtgg ggcaaaggaa
     2041 ccgtcttcaa cttcgagggc ggctgctacg ccaagtgcat cgacctgacc cgggagaatg
     2101 agccgctcat ctacgatgcg atccggttcg gcgccatcgt cgagaacgtc atcatcgacc
     2161 aggacacccg cgaacccgac tacacggacg gatcgctgac cgagaacacc agggcctgtt
     2221 atccgcgcag caacatcgaa gccaaggtga ccgacaaccg tggcggcgaa cccggccagg
     2281 tggtatttct cacctgtgac gtgagcggcg tgctgccgcc ggtttccctg ctctccaagg
     2341 aagctgccgc ctaccacttc ctgtccggct acacggctca ggtggggtcc acggaagtgg
     2401 gctctacgga agcctacaag gcaaccttct ccacctgctt cggggcgccc ttcttcccgc
     2461 gtgcggccgg tgtgtacgcc gatctgctga tcaagcggat cgaggagttc ggctccaagg
     2521 tgttcctggt caacacgggc tggaccggcg gcggctacgg cgttggcaaa cgcttcgcca
     2581 tcccggacac gcgggccatc atccgggcca ttcagtccgg cgccctggac gacgccgaga
     2641 cccggcgcct gccaggtttc aacctggaca ttccgacatc ggtgccgggc gtggacgcag
     2701 gtcttctcaa cccgagagat acgtggtccg acaaggccgc ttacgacgaa tccgcgcgca
     2761 gcctgatcgc aaaattcgca gagaacttcg aaaagttcag cgtcgctcag gccatcgttg
     2821 atgctggtcc gaccacggat tattgaggta gcctgaaggc cgccccgttg ggctaagagg
     2881 cggatatggc ccacgcagcc gcacccatcg acccggcgct cctggacgca ttgcgcgacc
     2941 agccggcttc cctgcgagag atccagcggg gcatcgagaa ggaaagcctg cgggttacgc
     3001 cggaaggctc gctctcagac cgaccgcatc cggccgccct gggctccgcg ctcacccatc
     3061 cgaacatcac cacggacttc agcgaggctc agctggagct gatcaccgaa gtgcatcagt
     3121 ctcctgaggc gacgctcgcc cagctggagg gcgttcaccg gttcgtctac cagaacctgg
     3181 acggcgagct gctctggtcc gccagcatgc cgtgtgtgct cggcacggac gaggagattc
     3241 ccgtaggtcg ctacgggggc tcgaacatcg ccagggccaa gacgatttac cggctggggc
     3301 tcggtaatcg ctacgggcgg ctgatgcaga ccatctcagg catccactac aacttttcgg
     3361 tcccgcagac gttctggaag gtgctggcgg aaagcagggg gaaagagccg acgcaggaat
     3421 tcgtaacgga agcgtatttc ggcctcatcc gcaatttccg gcgcttttcg tggctgctca
     3481 tctacctgtt cgggtcgtcg cctgcgatct gcaaatcgtt cgtgaaaaat cagccccatg
     3541 agctggcgtc cttcgacgaa ggctcgctct accttccaca cgccacgtcg ctgcgaatgg
     3601 ggcgcctggg ctaccagagc gacgctcagc gcagcctcca catttcctac aacggtctcg
     3661 agcagtacgc ggcatccatg cacacggcgc ttacccgacc ttaccccccg tacgaggcca
     3721 tcggcgtcaa agtcgacggc gagtaccgac agctgaacgc ctcgctgctg cagatcgaga
     3781 acgagttcta cggaaccatc cggccgaaac gccgcatcgt gagcggcgaa aggcccctga
     3841 acgccctgag atcccgcggg gtcgagtacg tcgaggtcag atgcctggac ctgaacccgt
     3901 ttctccacat gggcatagac accgtcgaga tccgcttcat cgacgcgttt ctgctttact
     3961 gcctgctggc ggacagccct gaggacagcg agcgggaatc ggagttgatg gggaaaaacc
     4021 agctcgccgt ggtcgagcgg ggccgcgagc cggaccttca attggagcag ctgcgcggca
     4081 ccatcacacg aaaggcatgg gcggaagacg tcctgcacgc ctgccgtccg ctcgccgagc
     4141 ttctggacga ggccaccggg agcgaactgt accgccacgc gctcagcgag cagtccagca
     4201 agaaccggca gccggcgctg acgccgtcgg cgcgggtgct ggccgtcatg cagacccagg
     4261 gcataccgtt tttccgcttc gccatgaacc agtccattgc ccacaagggt tacttcgacg
     4321 agcacccgat gcgaggtgag cagctggccg agcaccaggc cacctcgcaa cagtccctgg
     4381 cgcggcagca ggagatcgag gccgccgaca gcgtggattt cgacgtcttc ctcaaggact
     4441 acctggctct cgagtgaact ttctcgccca ctgcctgatc gctgcgcaat ccaccgaccc
     4501 cgaggaaccg gacctcgtcg ccggcggctt tctgggcgac ttcgtcaagg gccccattgg
     4561 ccccgaactg cccccgggtc tggctctcgg cgtacggctg catcggcgca tcgacgcctt
     4621 cagcaaccgg cacccggcca tccaggggag ctgcaatcga tttgcgggcc ccctgcgccg
     4681 gctggcgccc gtgctcgtcg acatcatcgc cgaccactgc ctggccaagc actgggagca
     4741 gttcagcaac gagcccatcg cagccttcac cgcaaggacc tacgatcgca tcgcgtccca
     4801 tgagcgctgg ctccccgacc acggccaccg cttcctcagc tacatgcgcg acgaagacct
     4861 gctggcgggg tacgcccggt tcgaagccat gcaccgggct ctgtgctcgg tgacacgtcg
     4921 cctggatcgc gagcacctca acgccgacat gctcagcgct accgaggcaa acctgcccgg
     4981 gctggaggcc gatttcctgg ggtacttccc ggaactggtg gcccatgcac ggcagtggtt
     5041 gcgcgacgag ggcccgcccc ccggacccca aacgccgggc acggtgcagc gctgaaaccg
     5101 gtactgccag gttgacgctg ccgaaccgac cgttactctt gcgttctttg attttcagga
     5161 agcatccatg aaagcggttc tgtgcaaggc attcggccca ccggaagacc tggtgatgga
     5221 ggaggtggca aatccggagc ctgggcccgg ccagatcctc gtcgacattc atgcctccgc
     5281 cctgaacttc ccggacgtgc tgatgatcgc cggcaagtac cagagtctcc caccgttccc
     5341 tttctctccg ggcggcgaga tcgcgggaac cgttgccgcg gtcggcgacg gcgccacgcg
     5401 tttcaaggtc ggcgaccggg tctttggagg gaccggccac ggcggcttcg ccgagcagat
     5461 cgccctgccc gagatggcca tgcggcccat gccggacgag atgagcttcg tcgaggcctc
     5521 cgccatcagc acgacctacg gcacctccta ctacgctttg aagcagcggg ccgatctcaa
     5581 gccgggcgag accctgctgg tcacgggagc gggcggcggc gtggggctgg cggccgtgga
     5641 gctcggcaag gccatgggcg cacgggtaat cgccgcggcc agctctgccg agaagctcgc
     5701 cgcggccagg agcgccgggg ccgacgagct catcgactac agcgatgggg agctcaagga
     5761 gaaggtcaaa gcactcaccg acggcaaggg ggcggacgtc atctacgagc ccgtcggcgg
     5821 cgatctgttc gagcagtgca tgcgctgcgt gaactggtat gggcggattc tggtcatcgg
     5881 cttcgcggcc ggtgacatcc cccgcgtacc catcaacctc attctgctca agagctgtca
     5941 ggtggttggg gtcttctacg gcgcctggag cgcgaggtgc cgcgacgagg ccgacgccaa
     6001 cttcagggag atcctggacc tgtaccggga cggcaagatc aagccgatga tcggccgtac
     6061 ctacccgctg gaggagtatg cggccgcgct gcgctgcctg a
//
LOCUS       DBNHKV010000127         7503 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_371788, whole genome shotgun sequence.
ACCESSION   DBNHKV010000127 DBNHKV010000000
VERSION     DBNHKV010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7503)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7503)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7503
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_371788"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="AC16PP_03985"
     CDS             <1..328
                     /locus_tag="AC16PP_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480815.1"
                     /translation="DCGLSVILEFSAPKTIGLETSQRLSPFHIINRAGSVLEVLREVV
                     PAEIQIDVVDLLPNRQPQAGARYCPKSQTRILVSKKLPSTLRALQIAAGSAAHRVCQS
                     VRNAPQ"
     gene            complement(473..1987)
                     /locus_tag="AC16PP_03990"
     CDS             complement(473..1987)
                     /locus_tag="AC16PP_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014756484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6480816.1"
                     /translation="MPIKRPSHGELRSVAQRLNLTLSENDVETFRDLMGASIDAFNQV
                     EAMDDELPPVRYPRDSGTRPSAADNPLNAWYYRTSIQGADGGALAGRTVALKDNIMLA
                     GVPMMCGSATLEGYVPEIDATVVERILDAGGEIAGKAHCEFFCLSGGSHTNSTGPVHN
                     PHKRGYSAGGSSSGSAALVAAGDVDMAIGGDQGGSIRIPASFCGVYGMKPTHGLVPYT
                     GVMPLEVFVDHIGPMTANVEDNATLLEAIAGPDAYDPRQRGVETHRYTEALGQDIAGL
                     KIAVLKEGFGHPNSESDVDDCVRAAAAHFESLGARVDEVSIPIHLDAQAIAMPILNYG
                     IVQTMLEGDGFGGGRNDLYVTSLMEFHRGWRGRIDEFSDNAKLWALIGGYVTERVGMH
                     HYGKAVNLSRKVRAAYDAVLEDCDLILAPTLPIKATPLPAADTGRAEKVARAMEMFAN
                     TVAADITHHPSMSVPCGLREGLPVGMMLTAKHFAETVIYRAAHAFERSKDWRTM"
     gene            2201..3403
                     /locus_tag="AC16PP_03995"
     CDS             2201..3403
                     /locus_tag="AC16PP_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6480817.1"
                     /translation="MGSDFDHLHGDFNIEDSAFAEQFEDVLEHLVSKCPVARSQVGPG
                     YRVFNRYRDVRQIGQDWRTFSSADGWMLDPPEGNIPILPEDCDPPYHTAWRQALNPFF
                     NAKEVAKLEDHARGYARELIDGFADRNRCEYVADFAAKLPGLILFRHILPVPVQDLAE
                     LFADIDTYSFGPLEERAPAFGRVYAYLEAFLDTRQHQGPQGDLVDVLLAGVERDGAPC
                     PWEDKVHTALDVVFGGLATTTHAMSGAMYEMARRPEVRAAVMGDPERLGTAVEETVRL
                     YAPVVMVARSVRSDVNVGGVEIASGERVALNYAAASRDPEVCSNPREFDVNRHEVVHT
                     AFGIGPHRCIGEHLARLEIRVAVEEFLKRIPDVSLESDGAPTYESGQLRTMKELRLCW
                     SAASASSF"
     gene            3424..3612
                     /locus_tag="AC16PP_04000"
     CDS             3424..3612
                     /locus_tag="AC16PP_04000"
                     /inference="COORDINATES: protein motif:HMM:NF028481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR6480818.1"
                     /translation="MAGIEFQTRSLGIAFAETAPKGRLPYIEHDGAQIAGSSISRPAH
                     TRRCAGQVEHRGVVRGRV"
     gene            complement(3782..4651)
                     /locus_tag="AC16PP_04005"
     CDS             complement(3782..4651)
                     /locus_tag="AC16PP_04005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6480819.1"
                     /translation="MSKRLGVSQTAIEIINGRWTMHIFGMRNDPATAELMSVLPNTSV
                     VGLWLTLFPLWVQFKLSGQLSFYPRIPKEGNETLADLVPSRTLYFDRLIRRALQNVEQ
                     FVLMGAGYDTRGYGMFADAGVTIFELDQPAVQSHKREMLQKTTINSNHVNFVTIDFSE
                     DDLFAKLADAGFDPSKKTLLLWEGVTLYLSLTEVQRTMRLVKRRAAPGSILLADLYAE
                     RMIELYGKSRVGAKLLELTDEGLDFGLSFAANWEQALNEFVEAQSMKVGEAHFLGSNN
                     EKGPYAVVAEMKC"
     gene            complement(4923..5117)
                     /locus_tag="AC16PP_04010"
     CDS             complement(4923..5117)
                     /locus_tag="AC16PP_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480820.1"
                     /translation="MKKSPLQIIQGIDVLQSSAKDLDQLRIGSEGAMMAHVAVDDGLV
                     ALCPRSIGILTNLNRQIPKG"
     gene            complement(5370..5651)
                     /locus_tag="AC16PP_04015"
     CDS             complement(5370..5651)
                     /locus_tag="AC16PP_04015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6480821.1"
                     /translation="MAFDGQRCIGNGRLLSDGVGNAYVIDVWTQSDYRRQGVASRIMG
                     MLIGGVPGQHIYLQTDNAIGFYKSLGFREQPTGMGMIAGDYLRNSSRDE"
     gene            6164..6379
                     /locus_tag="AC16PP_04020"
     CDS             6164..6379
                     /locus_tag="AC16PP_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR6480822.1"
                     /translation="MEANMATGTVKWFNPTKGFGFIQPESGDKDVFVHISAVEKAGMA
                     ALTEGQRVSYELITEKGRSSAGNLQAT"
     gene            complement(6406..6603)
                     /locus_tag="AC16PP_04025"
     CDS             complement(6406..6603)
                     /locus_tag="AC16PP_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480823.1"
                     /translation="MGEEELRSQLPGVIAFFRFSLSVSEALLSRCHWAGVVLAIFEKL
                     KIGWRPMRLAGGGELESSPCR"
     gene            6767..>7503
                     /locus_tag="AC16PP_04030"
     CDS             6767..>7503
                     /locus_tag="AC16PP_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HIR6480824.1"
                     /translation="MSDYTKILLEEHEMPTRWYNIIPDLPEPPPPPLHPGTQQPATAE
                     DFAPLFPQALIAQEMSRERYIDIPEPVLEVYRLWRPSPLFRARRLERFLETPARIFYK
                     YEGVSPAGSHKPNTAVPQVWYNAQEGITKLTTETGAGQWGSSLAFACAQFGLECEVWQ
                     VAASYHAKPYRRTMMEIWGGKVHPSPSEVTEFGRSLLAENPDHPGSLGIAISEAVSQA
                     VADPQTRYALGSVLNHVLLHQTIIGEEA"
BASE COUNT         1572 a         2273 c         2138 g         1520 t
ORIGIN      
        1 tgattgcggc ctgagcgtga tcttagagtt ttcagcccca aagacaatcg ggctcgagac
       61 atcccagcga ttgagcccat tccacatcat caatcgcgcc ggctccgtac tggaagtgct
      121 gagagaggta gtaccagcag agatccagat tgatgtagta gaccttctcc ccaacagaca
      181 accacaagcg ggtgcccgct actgcccaaa atctcaaacc cgcattctag tatcgaaaaa
      241 attgccgtcc acgctgcgtg cattgcagat tgctgcaggt tctgccgcgc atcgggtctg
      301 ccaatccgtc cggaacgcgc cacagtgatc ggacgactga caggttacca gtcagcgagc
      361 gaacatctgc catgcaccat ggaaatgacc gcttcagaac aatcaaggtc ccatgggggg
      421 ccacacgctg ccttatcaat tatctcgacg ttcatggagc gcttggggtc ccctacatcg
      481 tccgccagtc cttcgaacgc tcaaaggcat gggccgcccg gtaaattacg gtttctgcga
      541 agtgctttgc cgtgagcatc attcccaccg gaaggccttc gcgcaatccg cacgggacgc
      601 tcatcgatgg gtggtgcgtg atgtctgcgg caaccgtgtt ggcaaacatc tccatcgcgc
      661 gcgccacctt ctctgcccga ccggtatccg cagccggaag gggtgtggcc ttgattggca
      721 gcgtcggcgc cagtatcagg tcgcagtctt ccagaaccgc gtcgtaggcc gcccgcacct
      781 tgcgactgag gttgaccgcc ttgccgtagt gatgcatgcc gacacgctcg gtaacgtagc
      841 cgccaatcag cgcccaaagt ttggcgttgt cggagaactc gtctatccgt cctcgccagc
      901 cgcgatggaa ctccatgagg ctggtcacgt agaggtcgtt gcgaccgccc ccaaacccat
      961 cgccttcgag catggtctgc acgatgccgt agttgagaat gggcatcgct atcgcctgcg
     1021 cgtcgagatg gatgggaatc gagacctcgt ccacccgcgc gccaagggac tcgaagtgcg
     1081 ccgcggccgc gcgaacacag tcatccacgt ccgattcgga gttggggtgc ccaaaccctt
     1141 ccttcagcac ggcgatcttc agccccgcga tgtcctgccc cagggcttcg gtgtaacggt
     1201 gcgtttcaac gcctctctga cgcgggtcat acgcgtcggg acccgcgatc gcctcgagga
     1261 gcgtcgcgtt gtcctctacg ttcgcggtca tcggaccgat gtggtcgaca aagacctcca
     1321 agggcatcac gcccgtatag ggcaccagac cgtgcgtagg cttcatgccg tacacaccac
     1381 agaaagacgc gggaatgcga atcgaaccgc cctgatcgcc gcctatcgcc atgtccacat
     1441 cgcccgccgc caccagggct gcgctgccgg aggacgagcc acccgccgag taaccacgtt
     1501 tgtgcggatt gtgcaccgga ccggttgagt tggtgtggct gccgccggac aaacagaaga
     1561 actcgcagtg cgctttgccg gcgatctcgc cgcccgcgtc cagaatgcgt tccaccaccg
     1621 tcgcgtcgat ttcgggcacg tagccttcca gggtggccga gccgcacatc atcgggacgc
     1681 cggcgagcat gatgttgtcc ttgagcgcca cggttcgtcc cgcgagcgcc ccaccatccg
     1741 caccttggat gctggtacgg tagtaccagg cgttgagtgg gttgtcagcc gcactcggcc
     1801 gcgtcccgga gtcccgcggg taccgcaccg gtggcagctc gtcatccatc gcctcaacct
     1861 gattgaacgc gtcgatcgac gcccccatca ggtcccgaaa cgtctcaaca tcgttttcgg
     1921 acaaggtgag gttcagacgt tgagcaactg atcgcagttc gccatgactg ggtcttttaa
     1981 tcggcatgag cacctccttt gattcgcttc cgagcacacg gttgccaagc ctggcggagc
     2041 aatcgccaat ggtccccgcg ctggacccag attacgcgtc gcgccgaagc tggtcgagcc
     2101 actcccttgc agcatgagtc ttgccatctg aagggcttgc gcccgcagca gaagtgcact
     2161 aggaagaagg gctgaaggac tgtcatgagc ggggagcgag atggggtctg acttcgacca
     2221 cttgcacggc gatttcaata tagaagacag cgcgttcgca gagcaatttg aggacgtgct
     2281 ggaacatctg gttagtaagt gtccggtcgc acgcagccag gtgggtcccg ggtatcgcgt
     2341 gttcaaccgc tatcgcgatg tacgccagat aggccaggac tggcgcactt tcagcagcgc
     2401 ggatggctgg atgctcgatc cgccggaagg caatattccc atcttgccgg aagactgcga
     2461 ccctccgtat cacaccgcct ggagacaggc gctgaatcct tttttcaatg ccaaggaagt
     2521 ggctaagctc gaagatcacg cgcgcggcta tgccagagaa ctgatcgatg gctttgccga
     2581 tcgtaatcga tgcgagtacg tcgcggactt tgctgcgaag cttcccggat tgatcctgtt
     2641 ccgacacatc ctgcccgtcc cggttcagga ccttgctgaa ctgttcgccg acatcgacac
     2701 ctactcgttc ggcccgttgg aagagcgtgc gccggccttt ggccgtgtct acgcctatct
     2761 agaagcattt ctcgacacgc gccagcacca ggggccccag ggagatctgg tcgacgtgct
     2821 gcttgctggg gttgagcgtg acggcgcgcc atgtccgtgg gaggacaagg tgcacacagc
     2881 gcttgatgtt gtgttcggcg gactcgcaac gaccacccac gcgatgtcgg gcgcgatgta
     2941 tgagatggcc cgacggccgg aggtgcgggc ggccgtgatg ggcgatccgg aacgtttggg
     3001 cactgcggta gaagagaccg tacgacttta tgcccccgta gtcatggtgg cacgcagcgt
     3061 tcgctcggac gtcaacgtag ggggcgtgga aattgcgtca ggagagcgtg ttgcactgaa
     3121 ctacgcggcc gcgtcgcggg accccgaagt ttgcagcaat ccccgcgagt tcgacgtgaa
     3181 tcgtcacgag gtggttcaca ctgcattcgg aatcggcccg catcgttgca ttggagaaca
     3241 cctggctcgg ctcgaaattc gggtggcggt ggaggaattc ctgaaacgta ttcccgacgt
     3301 gtcgctggaa tccgatggag cacccactta cgagagcggt caactgcgga cgatgaaaga
     3361 gcttcggctt tgctggtcgg ctgcatcagc cagttcgttc tgaaagtcga gacctgcctg
     3421 cgcgtggcag gcatcgaatt ccagaccagg agcctcggaa tcgccttcgc tgaaacggct
     3481 cccaagggca ggcttccata catcgagcac gacggcgctc agatcgccgg ttccagcatc
     3541 tcacgacccg cacacacccg acgatgcgcc ggacaggtgg agcatcgagg tgtggtgcgt
     3601 ggtcgtgtat gaggcttgcg gcctgctgcg cctgctgaca aagatcctgc tcgaacctga
     3661 gcgctccgcg ctggtgtcac ctcgatagac gcttgaaggt atcgccccct atccccggag
     3721 cctggacgcc gcctgggagg tctcctacgc gtgatcagca gccatagtta gagcaatccg
     3781 atcagcactt catttccgcg actaccgcat aagggccttt ttcgttgttg ctccccagga
     3841 agtgagcttc gccgaccttc atggattgag cctccacaaa ttcgttcagc gcttgctccc
     3901 agttggcagc gaaggaaaga ccgaaatcga ggccctcgtc ggtcagttcc agcaacttcg
     3961 ctccaacgcg tgactttcca tacagctcaa tcatccgctc tgcatacagg tcggccaaca
     4021 ggatgctgcc gggtgcagcc cgtcgtttca ccaaccgcat ggttcgttga acttccgtga
     4081 gggacagata gagagtcaca ccttcccaga gaagcagcgt cttctttgaa ggatcaaacc
     4141 cagcgtccgc taactttgcg aacaggtcgt cttcgctgaa atcgatggtg acgaaattga
     4201 cgtgattgct gttgatggtc gtcttttgca gcatttcccg cttgtgcgac tgaacagcgg
     4261 gctggtcgag ctcgaaaata gtcacgccag catcggcgaa cataccgtaa ccgcgggtgt
     4321 catagcctgc acccattagc acaaactgct ccacattctg taacgcacgt ctaatgaggc
     4381 gatcaaaata aagggtgcgt gaaggcacca ggtccgcgag cgtttcattc ccctccttcg
     4441 gaattcgcgg atagaaggaa agctgacccg acagcttgaa ctgcacccac agtgggaaca
     4501 acgtcagcca caaccctact actgacgtgt taggcaacac cgacatgagc tccgcagtgg
     4561 cgggatcatt gcgcattcca aatatgtgca tggtccatcg gccgtttatg atttcaatcg
     4621 cagtctgaga aacgcccaga cgcttgctca cccataactg tctatagcct gtcagagcaa
     4681 taccaatcac cgcgagcgga aagtaccggc attgagtcgg aagggaatgt ttcggttagt
     4741 gatcgccaag ttctgctatc gggcaggagc ggactttttg atatggtaag cggcatggcc
     4801 ggttacattt tcagttccca attaagacta aaacggatag ccgttagcac tcgcgaaaga
     4861 tggatataaa ctgggaagca gttggagcaa tcggagagat cgtcggtgct gtcgcggttg
     4921 ttctaaccct tgggtatctg gcggttcaga ttcgtcagaa taccaatgct tcgcgggcac
     4981 agagctacca agccgtcgtc gacggccacg tgggccatca tcgcaccttc acttccgatc
     5041 cgaagctgat cgagatcttt ggcagaggac tgcaggacat cgataccctg gatgatctgg
     5101 agcggggact ttttcattct ctggttgctc caatcgtatt gcagtatcag aagtatctct
     5161 atttatatag agcgggcatg ctgccgcccg atacctttga catgttcgag cgtgatgtga
     5221 tcgcgacgct catcacgccc ggcggccggg catggtggct gagctcgaag gggaaatggc
     5281 cggagatatc aaaccacctg gaccaacgaa tcgaagacct gcaaagtgag gtcgttccaa
     5341 gccacgaaga ctttggcggc cattacccat cattcgtccc gagagctatt gcgaaggtaa
     5401 tctccggcaa tcatgcccat accggtaggt tgttctcgaa agccgagcga cttgtagaag
     5461 ccaatggcgt tgtccgtctg aaggtagatg tgctgacccg ggacgccgcc aatcagcatt
     5521 cccatgatcc tcgaggcaac accctgacgg cggtagtcgc tttgtgtcca cacgtcgatg
     5581 acataagcgt tcccaacacc gtctgacaac agccgcccgt tgccgataca tcgctggccg
     5641 tcgaatgcca ttgcgacaca ggcactgttc gcaaacgaca ggcgtagctg cgcagttgtc
     5701 ctgccgttgt gaaagtcgtc agcgatgagg tcttttcgca actcctccca gtccaccctt
     5761 cccagatcag atgtgtagtc gatcaagcca atctcctccc gggtatcctc aagcgcttcg
     5821 tatgtttacg ttcgccgaaa agcaatgact ccttttggag tcggaaacag actaccgcac
     5881 cgcagactgg aaacgtccct ttaaggcagg aaaccgacgt caggattcag ccgcaagaag
     5941 tccgccgtcg gccggaagca ggccaactcc cggcttgcct gacacttgaa gattgatgcg
     6001 caacgcaaag gaacttcttg tgctgcagcc ttaattcgcg taccgttcgc acgcgatcta
     6061 cattttggcc tgccttctgc acggcccaga catgggcttt cgtttcaaga cacccactat
     6121 cgagattgtt cgtcaccgcg cccgatttgc gcgcggcatt gctatggagg ccaatatggc
     6181 tacaggaact gtaaagtggt ttaaccctac caaagggttt ggattcattc agccggaatc
     6241 cggcgacaaa gatgtgttcg tgcacatcag cgcagtagag aaggcgggga tggcagcact
     6301 gactgaaggt cagcgcgtta gctatgaact gatcaccgag aaaggtcgct cctcagcggg
     6361 caacctgcag gctacctgat actgctgtcc ttcagcccgt gcaggtcacc tgcacgggct
     6421 gctttcgagc tctccccctc cagccaggcg catcgggcgc cacccgatct tcagtttctc
     6481 gaagatcgcc agtaccacgc ctgcccaatg acaacgactc agtaaagctt cgctgacgga
     6541 cagggagaac cggaagaacg cgatcacgcc aggcaattgc gatcgcagct cttcttcgcc
     6601 caaccagcca ttcagcggca cgcgagcgtg gtaacccccc gatcgcgctg gtcaccacca
     6661 cttacggctc tggctggaga tctttcgttt tagccgaacc cgccctatga tcgggcgttt
     6721 tgcttgccgg acctccagct tcgcaggcgc agacggacac tgaagtatga gcgactacac
     6781 caagatcctg ctcgaagagc acgagatgcc gacgcggtgg tacaacatca ttcccgacct
     6841 gccggaaccg cccccgccgc ccctgcatcc gggtacccag cagccggcca ccgcagaaga
     6901 cttcgcgccc ctgtttcccc aggcgctgat agcccaggag atgagccggg aacgctacat
     6961 cgacattccg gaacccgttc tggaggtcta ccggctgtgg cggccaagcc cgttgttccg
     7021 agcacggcgc ctggaaaggt tcctggagac gcctgccaga atattctaca aatatgaggg
     7081 cgtgagcccg gcgggttccc acaagccgaa caccgccgtt cctcaggtct ggtacaacgc
     7141 ccaggaaggc atcaccaagc tcaccaccga gaccggcgcc ggccagtggg gcagctcgct
     7201 cgcctttgcc tgcgctcagt tcggcctgga gtgcgaggtc tggcaggtgg cggcttccta
     7261 tcatgccaag ccttaccggc ggacgatgat ggaaatctgg ggcggcaagg tgcacccgag
     7321 cccctctgaa gtcacggagt tcggccgttc cctgcttgcc gagaaccccg accatcccgg
     7381 atcgctgggg atcgcaatct ctgaagcggt gagccaagcg gtcgccgatc cgcagacacg
     7441 ttacgcgctc ggcagcgtgc tcaatcacgt gctgctccac cagacgatca tcggagagga
     7501 agc
//
LOCUS       DBNHKV010000128         9789 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_18135, whole genome shotgun sequence.
ACCESSION   DBNHKV010000128 DBNHKV010000000
VERSION     DBNHKV010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9789
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_18135"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="AC16PP_04035"
     CDS             complement(<1..670)
                     /locus_tag="AC16PP_04035"
                     /inference="COORDINATES: protein motif:HMM:NF018961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HIR6480825.1"
                     /translation="MNTFEAIEDLLARCERLQDVAVDSLDEVCSSLGVQLARRFLSER
                     KHLYRRYLAFCLEDKELTEAEHADLAHLQHLLYLGAADVALIHDDVAHEVYGKALDEV
                     LSDLKISAEEEAFLRRLRSELKLSEAEAAVMLEAGRERMRDVALEQASTSDPEFSKPR
                     APIGQFVGRSDESLEQAVRDAVSKARLAIPRLHWFEVGNISGYVDDEGPKSWHVTVRG
                     GIKTS"
     gene            847..1686
                     /locus_tag="AC16PP_04040"
     CDS             847..1686
                     /locus_tag="AC16PP_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6480826.1"
                     /translation="MTTGRPVAVVIGATSKWQADGRNTRLAHGHDVDDSDLPVSARWG
                     IGGAVAQKFAREGFLVVLTTRTAANAAGLEAAIAEQGGECMTVELDLVSERSVSEAFA
                     TIRREAGEPEVVVYNAGYLEGRDLPPDQELLEHIPVEMFDTAQHISSRGPFLVAKEIL
                     PGMRERGSGSFLITNNAASLRGRKRYTGQSLYYPRVMMRTLAQVLTEEYSEHGVHVAN
                     IIVDGLIDSPGTRALPMAQQRPEIIMNPAAIADAYYYLHTQDRSCWTHELQLTPHSTK
                     PSF"
     gene            complement(1726..2529)
                     /locus_tag="AC16PP_04045"
     CDS             complement(1726..2529)
                     /locus_tag="AC16PP_04045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013692821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6480827.1"
                     /translation="MTANGLLENKIAVVVGGGQTPGETIGNGRATAIQFAREGARVLV
                     GDRDVGSAEETVAMILDEGGEAASAEIDVTVEESVASFMAAAVDRWGRIDVLHNNVGV
                     GLAGADAGVEEISADVFDQLVRINLRGMVLTAKHVLPGMRERGSGVIINISSMAAWKP
                     YPFVGYKTTKAGVIALTEQLAQANARYGIRANVILPGLMNTPMAVEPRVSQTMTREEV
                     IADRDRRVPLGRKMGTAWDVAHAAAFLASDRARFITGVALPVDGGASVS"
     gene            complement(2572..3177)
                     /locus_tag="AC16PP_04050"
     CDS             complement(2572..3177)
                     /locus_tag="AC16PP_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480828.1"
                     /translation="MSEALSPSPRPTLAGWIIRDVLITSGLAAMLLGLAAWHEAEGSL
                     AAALVAPALGFVAAYVLCYVYHEWGHLIGARLTGAAMPLAPYAGALIGHFDIQAHSRR
                     QFLWLSWGGVIAYVALMSIALSVYLHGGLGPAGAGFAVGGLAFVVQSLSVDVPQIVRV
                     MRGDDIARTSAEGANARVILRRTWQSWLVLAGALIVWNFAS"
     gene            complement(3187..4356)
                     /locus_tag="AC16PP_04055"
     CDS             complement(3187..4356)
                     /locus_tag="AC16PP_04055"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6480829.1"
                     /translation="MTWSPFAPEVMADPANGHRELLDRCPVHHCEDFDPPFFTLSRYA
                     DVEEALRDIETFSSHYGQGPRFTEPQGMLCDPPQHTYMRKLVQQAFTPKAMEALRIQV
                     ADLTEKLIDDILAGEPEFDLHDDFAFPLPVIIIAGMLGVPTEDLDRFKHWSDVQVAAM
                     GSSDPTRFEADQAAFFEYMVNHYRKRHAAIVAGEAVPDDLLTMIAGARADDGSFIAEA
                     DAQSMLTQLLVGGNETTTSLITNMVWRLLEDRERWQEVVADPVLVEAAVEESLRFDPP
                     VLGLYRNTTRDVVLHDTVIPTGSKVYINYAAANRDASVFERPDEFDLHREAKRHMSFG
                     LGVHFCLGAPMARLEAEIALKALARRMPDLKLLGPGQRIAPFFLWGRRNLPVAWR"
     gene            4505..6634
                     /locus_tag="AC16PP_04060"
     CDS             4505..6634
                     /locus_tag="AC16PP_04060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR6480830.1"
                     /translation="METAKSYCRYCHAYCAIDVDVEDGRPVAIRGDAKDPVYGGYTCI
                     KGRQLPELYDAPDRLHTALKKMPDGRFEEIPTARALDEIAARIERIIERDGPRAVASY
                     CGTYAFMESAALALARSFHQGIGSESFYTSVTIDQPGRPIAMSRFGTWGGGTHSFADA
                     DVVMSIGNNAVVSSFSVFGGIPPFNPYRRMNDAIKDGKKLIVIDPRRTEVARKAHLFL
                     QVRPGEDPTLLAGIIRVILDEDLHDQPFCDQYVDGLDAIKAAVDPFTLEYVARRAAVP
                     AEQIAEAARTFAAGPKGVAVTGTGPNMAERAPLTEHLVLALNTICGRVNRAGERLPNP
                     GILSPLTAVKAEVIPPAPAWGHGPRNRVRGLGQIFGEMPTAALSDEILEPGEGQVRAL
                     LSIGGNPIVAWPDQIKTKRALDALDLLVCVDIRLSATAQLADYVIPGKICLEREDMPV
                     LTDNWYDVPYGHYTEAVCQPNGDLREEWEFYWELAHRLNSDLPLPGGSLPTQEKPGKQ
                     TVLEHMFPNPKVPIADIRAQDGGHVFEGVQVMVEPGQGDARLQLTPGGVADELAEVRA
                     ETLQENGAPGKDGDYSHLLISRRLKHVYNSSGQQLPEIRKKGTTNPAYMNPEDVAALG
                     IGDGDVVQISSRHGQILGVAAAAPDVKRGVISMAHAWGGLPGNDGEVREIGASTNRLL
                     SNDKEYDPITGMARQSAIPVNVRPVAL"
     gene            complement(6658..7977)
                     /locus_tag="AC16PP_04065"
     CDS             complement(6658..7977)
                     /locus_tag="AC16PP_04065"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6480831.1"
                     /translation="METKKPNHELSPMRCPVHLEDVDLFSPGAAEHWYEAYEILHHEA
                     PVHRIPGEGFLPGTDGFILSKYEDIALVVREEERFPPAQTLIARQLLDKHIEPFDTPR
                     VNAAMGGVLVLRPTPETWRAHRQELTDPWVGPGAGRNEEMIRRTADELIDRWIDRGTV
                     DFVSEFAQPLPQMVMANILGWPLEDLKLLKYYGDGTVKPFVFGKGHRSVLPEEEVKSQ
                     FEVLEEFKTYTIALIEEKRRNPADDMITFLTEVEYSPVGRKLNDNEINSVVYNMVIGG
                     LETTQYALSEQVQLLIERDGVWDEIKADRSKVRGFTEDAMRLRAPTQGLSTRTTSRDE
                     VFQGVHVPKGSLLHLRWAAGNIDPDEWECPMDLKLDRRAGTRHLTFSQGARVCPGASL
                     SRLEQTVSWNALLDKVDRFAYGEDNNFMHQPGIMLGLEYLNLTFGKA"
     gene            8280..8756
                     /locus_tag="AC16PP_04070"
     CDS             8280..8756
                     /locus_tag="AC16PP_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480832.1"
                     /translation="MKNRIPFALMAALGFFAMNACADTEEVSSTAYWTIVAPAEEYKM
                     TNGATMTGGGIAHANVINNEGEHLSQWCRGITAADAEGKGLGGAGFCTIIDQAGDAMW
                     VWFSNNADGQGGTWGVIGGTGAYAGASGGGTSGAPTSMSPDGRSWINTSTGTITTP"
     gene            complement(8857..>9789)
                     /locus_tag="AC16PP_04075"
     CDS             complement(8857..>9789)
                     /locus_tag="AC16PP_04075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6480833.1"
                     /translation="MEHVVMQNEAIAWAAESGADVLRGVSGVAVTAGAKPRVRYTHDG
                     VDHDVGCRWIVGADGRGSSVRRQLDIAINEAPLDHLIAGLLIEGADDWPEDLQCLGKF
                     GDLHYLVFPQGGGKVRIYADYAYQGSARFSGPDGASEMLAAFDLPRVPHSKAVANARP
                     IGPCRSYPSQDAWVEEPCVEGAVLIGDAAGYNDPILGQGLSVTLRDAHSVGAILAGGD
                     AWSADLFRPYVEERGERLRRLRFAASFVTTLYARFEPGDVERRAEAFRRMGADPSLAV
                     MSLAAILGPETLPAECFTPAFYERAFGTTQNMVA"
BASE COUNT         1756 a         3287 c         3196 g         1550 t
ORIGIN      
        1 agctggtctt gatgccgccg cgcacggtga cgtgccagct cttcgggcct tcgtcgtcca
       61 catacccgga gatgttgccg acctcgaacc agtgcagccg ggggatggcg agccgggcct
      121 tggacacggc gtcgcgcacc gcctgctcca gggactcgtc cgagcgcccg acgaactgac
      181 cgatcggcgc tcggggcttc gaaaactccg ggtccgacgt cgacgcctgc tcgagtgcca
      241 catcgcgcat ccgctcccgg ccggcttcga gcatcacggc ggcttccgcc tcagagagct
      301 tcagctcgct tctcaaccgg cgcagaaaag cttcctcttc ggcgctgatc ttcagatctg
      361 acagcacctc gtccagcgcc ttcccgtaaa cttcgtgggc aacatcgtcg tgaataaggg
      421 ctacgtccgc cgcgcccaga taaagcaggt gctgcagatg ggcgagatcg gcgtgctcgg
      481 cttcggtgag ctccttgtcc tcgaggcaga acgccagata gcgccggtag agatgcttgc
      541 gctcgctcag gaaacggcgg gcaagctgaa cgcccaggct gctgcagact tcgtccaggc
      601 tgtcgacggc gacgtcctgc agccgttcgc aacgggccag aagatcctcg atggcctcaa
      661 aggtattcac gcgcttggat cggatgctgc gcgagaggcc ggggctggcc ggctgagccg
      721 ggcgaaaggg tgctgccacg tcgtgtttcc aatcgtttga ggtgattccc acggggggga
      781 catggtaccg gcggcgcttc atgggataca gtttcggaaa cagcacgaac acgggagatc
      841 gtagacatga cgactggcag accggttgcc gtggtcatcg gcgccacttc caagtggcag
      901 gccgacggcc gcaacacccg tctcgcccac gggcacgacg tggacgattc cgacctgccc
      961 gtctcggcgc gttggggcat cggcggcgcg gtggcgcaga agttcgcgcg tgaaggcttt
     1021 ctcgtggtgc tgaccacacg aacggcggcc aacgcggccg gcctggaggc ggccatcgcc
     1081 gagcagggcg gcgagtgcat gaccgtcgag ctcgacctgg tgtcggagcg gtccgtcagc
     1141 gaagccttcg cgaccatccg acgggaggcc ggcgagcccg aggtggtggt ctacaacgcg
     1201 ggctacctcg agggccggga cctgccgccc gatcaggaac tcctggaaca cattcccgtg
     1261 gagatgttcg acaccgccca gcacatctcc agccgcggcc cctttctcgt cgccaaggag
     1321 atcctgccgg gcatgcgcga acgcggctcc ggatcgttcc tcatcaccaa caacgcggcc
     1381 tcactgcggg gtcgtaagcg ctacacgggg cagtcgctgt actacccgag ggtgatgatg
     1441 cgcacgctgg cccaggtgtt gaccgaggag tactcggaac atggcgtcca cgtggccaac
     1501 atcatcgtcg acgggctcat cgactcgccg ggcacacggg ctctgcccat ggcgcagcag
     1561 cgcccggaga tcatcatgaa cccggccgcc atcgccgacg cctactacta cctgcacacc
     1621 caggacagat catgctggac ccacgagctg cagctgacgc cgcactcgac caagccaagc
     1681 ttctgagcct cgcctgcagg acaagcggcc cgcccagcca aagcctcagc tgacgctggc
     1741 gccgccgtcc acggggagag cgacccccgt aatgaaccgg gcgcggtcgg aggcaagaaa
     1801 ggctgccgcg tgggccacgt cccaggccgt tcccatcttg cgccccagcg gcacacgccg
     1861 gtcacggtcg gcgatgacct cctcccgggt catggtctgg gagacgcggg gctccacggc
     1921 catgggcgtg ttcatcaggc caggcaggat cacgttggct cgaatgccat agcgggcgtt
     1981 ggcctgggcg agctgctcgg ttagggcgat gacaccggcc ttcgtggtct tgtaacccac
     2041 aaagggatac ggcttccacg cggccatgga agagatgttg atgatcactc ctgagccccg
     2101 ctcgcgcatg ccgggcagaa cgtgcttggc ggtgagcacc atgccgcgca ggttgatgcg
     2161 caccagctga tcgaacacgt ccgccgagat ctcttccacg ccggcatcgg ccccggcaag
     2221 cccgacgccg acgttgttgt ggagcacgtc gatgcgtccc caacggtcga cggcggcagc
     2281 catgaacgag gcgacgctct cttccaccgt cacgtcaatt tcggcggacg cggcctcgcc
     2341 gccttcatcg aggatcatgg caacggtttc ctccgccgag cccacgtcgc ggtcgcccac
     2401 cagcacccgc gcgccctccc gagcgaactg gattgccgtg gcgcggccgt tgccgatggt
     2461 ctcccccggc gtctggccac cgcccacaac caccgcgatc ttgttttcca gcagcccgtt
     2521 ggccgtcatg tcagttctcc aggtagcggc gcgggcgagg tcgtcttcag ctcagcttgc
     2581 gaagttccag acgatcagcg ctcccgccag aacgagccag ctctgccagg tccggcgcag
     2641 gatgacgcgg gcgttggcgc cttcggccga cgtcctcgca atgtcgtctc cccgcatcac
     2701 cctgacgatc tgcggcacgt ccacggaaag ggattgcacg acgaaggcga gcccgcccac
     2761 ggcaaaaccc gcgcccgcgg ggccgagccc accatgcaga tagacggaga gggcaatgct
     2821 catcagggca acataggcga tcaccccgcc ccagctcagc cagaggaact ggcggcggct
     2881 gtgcgcctgg atgtcgaagt ggccgatgag ggcgccggcg tagggcgcca gcggcatggc
     2941 agcgccggtc agccgggcgc cgatcaggtg cccccactcg tgatagacgt agcagagcac
     3001 gtaagccgct acgaacccga gggccggagc gacgagcgca gcagccagcg aaccttccgc
     3061 ctcgtgccag gccgccaacc ccagcagcat cgcggcgagc cctgaggtga tgaggacgtc
     3121 gcgaattatc cagccggcaa gcgtcggccg cggcgatggc gagagggctt cggacaccgc
     3181 gccggactaa cgccaggcaa ccggcaggtt gcgccggccc cacaggaaga atggcgctat
     3241 gcgttgaccc ggcccgagga gcttcagatc cggcatgcgc cgcgccagcg ccttgagcgc
     3301 gatctccgct tcgagcctcg ccatgggggc gccgaggcag aagtgcacgc cgagcccgaa
     3361 cgacatgtgc cgcttggctt cccgatgcag gtcgaactcg tccggacgct cgaagacaga
     3421 ggcatcgcgg ttggcggcgg cgtagttgat gtagaccttg ctgcctgtcg gtatcacggt
     3481 gtcgtgaagc acgacatcac gggtggtgtt gcgatagagg ccgagcacgg gcgggtcgaa
     3541 acgcagactc tcctccacgg cggcctcgac caggacgggg tctgccacca cttcctgcca
     3601 gcgctcccgg tcctcgagga gccgccagac catgttggtg atgagcgacg tggtggtttc
     3661 gttcccgccc acgaggagct gggtgagcat ggattgcgca tccgcctccg cgatgaacga
     3721 gccgtcgtcg gcgcgagcgc cggcaatcat cgtcagcaga tcatccggta ccgcttcccc
     3781 ggccacgatg gccgcgtggc gcttgcgata gtggttgacc atgtactcga agaaggcggc
     3841 ctgatccgcc tcaaaacgcg tgggatccga cgatcccatg gccgctacct ggacgtcgga
     3901 ccagtgcttg aaccgatcga ggtcttcggt gggcaccccc agcatgccgg cgatgatgat
     3961 caccggcagg gggaaggcga aatcatcgtg caggtcgaat tccggctccc cggcaaggat
     4021 gtcgtcgatg agcttttcgg tgagatctgc gacctgaata cgaagcgctt ccatggcctt
     4081 gggcgtgaag gcctgctgga ccagcttgcg catgtaggtg tgctgaggcg gatcgcagag
     4141 catgccctga ggttccgtga accgcgggcc ctggccgtag tggctggaaa acgtctcgat
     4201 gtcccgcagc gcttcctcca cgtcggcata gcgggaaagc gtgaagaacg ggggatcaaa
     4261 atcctcgcaa tgatggacgg ggcatcggtc gagaagctcc cgatggccat tggccgggtc
     4321 ggccatgacc tcgggcgcga agggactcca ggtcatatcg accttcctga agtgcgggct
     4381 cgccagtata tcggcgtttg cctaattgga caccgcatgc gccagcgacc ggcaactcgc
     4441 cgggacttga atcgcacctc gccgtgcgca aagcttgatt cggcgaattc gggggaggac
     4501 gcggatggaa accgccaaga gctactgccg ctattgccac gcttactgcg ccatcgacgt
     4561 ggacgtggag gacgggcggc ccgtcgccat acgcggcgac gcaaaggatc cggtctacgg
     4621 cgggtacacg tgcatcaagg ggcgccagct gcccgagctc tacgacgccc ccgaccgtct
     4681 gcacaccgcc ttgaagaaga tgcccgacgg gcgcttcgag gagattccca cggcccgggc
     4741 cctggacgag atcgccgcgc gcatcgagcg gatcatcgag cgcgacgggc cgagagccgt
     4801 ggccagctac tgcggaacct acgccttcat ggagtcggcc gcactggcgc tggcgcgcag
     4861 cttccaccag gggatcggct cggagtcctt ctacacctcg gtgaccatcg accagcccgg
     4921 ccggcccatc gccatgtccc gcttcggcac ctggggcggc ggcacccaca gcttcgccga
     4981 tgccgacgtg gtgatgagca tcggcaacaa cgccgtggtt tcctcgtttt ccgtcttcgg
     5041 gggcatcccg cctttcaacc cctaccggcg catgaacgac gccatcaagg acggcaagaa
     5101 gctgatcgtc atcgaccccc ggcgcaccga ggtggcccgc aaggcgcacc tgttcctgca
     5161 ggttcggccc ggcgaggacc cgacgctgct ggcgggcatc atccgcgtga ttctcgacga
     5221 ggatctgcac gatcagcctt tctgcgacca gtacgttgac ggcctggatg ccatcaaggc
     5281 cgccgtcgac ccgttcaccc tggagtacgt tgccagacgc gcggccgtac cggccgagca
     5341 gatcgccgaa gccgcccgca cattcgccgc cgggcccaag ggggtggcgg tcacgggcac
     5401 cggccccaac atggccgagc gcgcgccgct gaccgaacac ctggtgctgg cgctcaacac
     5461 catctgcggc cgggtcaacc gggcgggcga gcggctaccg aacccgggca tcctgtcccc
     5521 gctgacggcc gtgaaagccg aggtcatccc gcccgcgccg gcctggggac acgggccccg
     5581 caaccgggtg cgcgggctgg gccagatctt cggcgagatg ccgaccgcag cgctgagcga
     5641 cgagattctc gagccgggcg aggggcaggt acgcgccctg ctctccatcg gcggcaaccc
     5701 catcgtggcg tggccggatc agatcaaaac caagcgggct ctggacgcgc tggacctgct
     5761 ggtctgcgtc gacatccgtc tctccgccac ggcccagctc gccgactacg tgattcccgg
     5821 caagatctgc ctggagcgcg aggacatgcc cgtgctcacg gacaactggt acgacgtgcc
     5881 ctacgggcac tacaccgagg cggtctgcca gcccaacggc gacctgcgcg aagagtggga
     5941 gttctactgg gagctggccc accggctgaa ttccgacctg ccgctccccg gcgggtctct
     6001 gcccacgcag gagaagcccg gcaagcagac ggtgctggag cacatgtttc ccaaccccaa
     6061 ggtacccatc gcggacatcc gcgcccagga cggcggccac gttttcgaag gggtccaggt
     6121 catggtggaa cccggccaag gcgacgcccg cctgcagctc acaccgggtg gtgtcgcgga
     6181 tgagctggcc gaggtgcgcg cagagacgct gcaggagaac ggcgcgccgg gcaaggacgg
     6241 agactactca catctgctga tcagccggcg actgaagcat gtgtacaact cctccggcca
     6301 gcagctgccg gagatccgca agaagggcac caccaacccc gcctacatga accccgagga
     6361 cgtggcagcg ctgggaatcg gtgacggtga cgtcgtccag atcagcagcc gccacggcca
     6421 gattctcggt gtcgcggcgg cagccccgga cgtcaagcgc ggcgtcatct ccatggccca
     6481 tgcctggggc ggcctgcccg ggaacgacgg tgaggtacgg gaaatcgggg ccagcacgaa
     6541 ccggctgctc agcaacgata aggaatacga cccgatcacg gggatggcgc ggcaaagcgc
     6601 aattcccgtg aacgtgcggc cggtggcgct ctagcgcccg gtcgctggcg ccggccgtca
     6661 ggccttgccg aacgtcaggt tcagatattc caggccgagc atgatgcccg gctggtgcat
     6721 gaagttgttg tcctcgccgt aagcgaagcg gtcgacctta tcgagcagag cgttccacga
     6781 aaccgtctgc tccaggcgcg acaggctcgc ccccgggcac acccgcgcgc cctgggagaa
     6841 agtcaggtgt cgggtgccgg ccctgcgatc cagcttgagg tccatcgggc attcccactc
     6901 gtccggatcg atgttgcccg ccgcccagcg cagatgcagc agcgaaccct tcggcacgtg
     6961 cacgccctgg aacacctcgt cccggctggt ggtgcgggtc gaaaggccct gtgtgggcgc
     7021 gcgcaggcgc atggcgtcct cggtgaatcc cctgaccttg ctgcgatcgg ctttgatctc
     7081 gtcccagacg ccgtcacgct cgatgagcag ctgcacctgc tcggacagcg cgtactgggt
     7141 cgtttccagg ccgccgatca ccatgttgta gacgacgctg ttgatctcgt tgtcgttgag
     7201 cttgcggcca accggcgagt actccacctc ggtgaggaag gtgatcatgt cgtcggccgg
     7261 gttgcggcgc ttttcctcga tgagcgcgat ggtgtaggtc ttgaactcct cgagcacctc
     7321 gaactgggac ttcacttcct cctcgggcag gacgctgcgg tggcccttgc cgaagacgaa
     7381 aggcttcacg gtaccgtcgc cgtagtactt caggagcttc agatcctcca gaggccagcc
     7441 caggatgttc gccatgacca tctgcggcag cggctgcgcg aactcggaaa cgaaatccac
     7501 cgtgccacgg tcgatccagc gatcgatcag ctcgtccgcc gtgcgccgga tcatctcctc
     7561 gttgcgcccg gctcccggcc ccacccaggg atcggtcagc tcctgacggt gggcccgcca
     7621 tgtctccggc gtgggccgca gcaccagcac gccccccatg gccgcgttga cccggggagt
     7681 gtcgaagggc tcgatatgct tgtcgagcag ctgccgggcg atcagcgtct gggccggggg
     7741 aaagcgctcc tcttcccgga ccaccagggc aatgtcctcg tacttggaga gaatgaaacc
     7801 gtccgtcccg ggcaggaagc cctcaccggg gatccggtgc acgggggctt cgtgatgcag
     7861 gatctcgtag gcctcgtacc aatgctccgc ggcccccggc gagaacaggt ccacgtcctc
     7921 cagatgcacc gggcagcgca tggggctgag ctcatgattg ggtttcttcg tttccaagta
     7981 acgtcctcct ggaagttgtc cccagaccca tcactgcccg gggcgaaggc ttggttgcaa
     8041 aatttgtagc atggtacaaa tcgctgggac cggcgtccaa gcccttatct gttggcgccg
     8101 catcatgaat ccgctttgtt gagcatcccg agatacgaag gcatagtgag ccagctgatc
     8161 ggaaatcagc aggggggaag gagcactcga aaccgggcgc tgaaggcact ctcaagcggt
     8221 tctgccacgc gctggtcacg cccgcacccc gaacaaccaa aaaaatcgag gaagacaaca
     8281 tgaaaaacag aattcccttc gcgttgatgg ccgcgctggg ctttttcgcc atgaatgcct
     8341 gcgcggatac cgaggaagtt agcagcacgg cctactggac gatcgtggcg cctgccgagg
     8401 agtacaagat gaccaacggc gcaacgatga ccggaggcgg catagcccac gccaacgtca
     8461 tcaacaacga aggcgagcac ctaagccaat ggtgccgcgg catcacggcg gcagatgctg
     8521 aaggcaaggg cctgggcggc gccgggttct gcacaattat cgatcaggcc ggcgatgcga
     8581 tgtgggtctg gttcagcaac aatgctgacg gtcagggcgg cacctggggc gtcatcgggg
     8641 gtaccggcgc gtacgccggc gcgagcggcg gtgggacgtc cggcgcacca acctccatga
     8701 gcccggacgg ccggtcctgg atcaacacgt ccaccggcac catcaccacg ccgtaggttc
     8761 ggcacgcccg gctttcccat ggaaagccgg gcgcacggtc ccttccaggc ccgatcatcc
     8821 aaaccccgca ccggaccgcc cgggcggcgc cggctgtcag gccaccatgt tctgcgtggt
     8881 tccgaaggcc cgctcgtaga aggcgggcgt gaagcactcg gcgggcagag tctcgggacc
     8941 caggatagcc gccagcgaca tgacggcgag gctggggtcc gcacccatgc gccggaacgc
     9001 ctccgcccga cgttcgacgt ccccgggttc gaaccgtgca tacagcgtcg tgacgaaaga
     9061 ggcggcaaag cgcaggcgcc gcagccgttc cccgcgttct tccacgtagg gccggaacag
     9121 gtcggcgctc caggcgtcgc cgccggcgag gatcgcgccc acgctgtggg catcgcgaag
     9181 ggtcaccgaa aggccctgtc ccaggatggg atcattgtag ccggccgcat caccaatcag
     9241 caccgcaccc tccacacaag gctcttccac ccaggcgtcc tggctgggat aagaccggca
     9301 gggaccgatg ggccgggcgt tggccactgc cttgctgtgg ggcacgcggg gcagatcgaa
     9361 ggcggcgagc atctcgctgg cgccgtcggg acccgagaat cgagcgctgc cctggtaggc
     9421 gtaatccgcg taaatcctca ccttgccgcc gccttgtgga aataccaggt agtgcaggtc
     9481 tccgaacttg ccgaggcact gcagatcttc cggccagtcg tcggcgccct cgatcaggag
     9541 cccggctatc aggtgatcca gaggcgcttc gttgatggca atgtcgagct ggcgccgaac
     9601 gctggacccg cggccatcgg cgccgacgat ccagcgacag ccgacgtcat ggtcgacgcc
     9661 gtcgtgcgtg taacgcacgc gcggcttggc gcccgccgtc acggcaacgc cgctcacccc
     9721 gcgcagaacg tccgcaccgg actccgccgc ccaggcgatg gcctcgttct gcatcaccac
     9781 gtgctccat
//
LOCUS       DBNHKV010000129         3678 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_868904, whole genome shotgun sequence.
ACCESSION   DBNHKV010000129 DBNHKV010000000
VERSION     DBNHKV010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_868904"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="AC16PP_04080"
     CDS             complement(<1..960)
                     /locus_tag="AC16PP_04080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6480834.1"
                     /translation="MTETYLTDERRMIQEVARDFTRREVLPVANELDPIQGDIPMDLR
                     DKLGEMGYFGIRMAEEHGGLGLGVFEYCLISEELARGWMSVASIIARATSLMNVGGWP
                     LEKREELTQKAARGEYLAAISLSEPDVGSDLAAAKCRAVLDGDEWVITGSKYWCTFAD
                     GADYIQLLARVEAPDSDDAQHKGLKNFIIEKPRGELPEGCSGSPIPKIGYFGWKTWEL
                     AFDGCRIPKENIITGGGGDGFKNAMHFLNAARAHTAARSIGLARGALEDAMAYSHERV
                     QFGVPIADFQETRFKIARMASEIEAARQLMYHAATLLDAGETAE"
     gene            complement(964..1875)
                     /locus_tag="AC16PP_04085"
     CDS             complement(964..1875)
                     /locus_tag="AC16PP_04085"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR6480835.1"
                     /translation="MNRQPKRARRCQLSVPGSSEKMMAKAAALDLDHVVLDLEDAVAP
                     SAKPDARGMIVDALNGLHWTPRTRCVRINDVETQWCHDDIIEVVTGAGEKLDTIMLTK
                     AKSAADVMFVHLMLDQLEQKLGLTRRIGIECLIEEVEGMMHVEEIAACSDRLECLVFG
                     MGDYSASQQMQLGHIGSSGGYPPDVWHYPRYKMTIACHANGIDPVDGPFANFRNPEAY
                     SKEAERAFVLGMVGKWAIHPSQIELANAVFTPDPAAIADARKQKAAYNEALSQGLGAI
                     NVDGVMVDAASIRIVQNLLDRAELIGM"
     gene            complement(1872..2375)
                     /locus_tag="AC16PP_04090"
     CDS             complement(1872..2375)
                     /locus_tag="AC16PP_04090"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039588.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6480836.1"
                     /translation="MALTGERNYFEDFQAGQVFRHARGKTMCEQDNVGITLQVLNTAE
                     GHFNEDVMQRNALALGDGRGGWNSRLQFGGVTIAMVIGLAMQDTGENALRELKLDKIR
                     LKAPVFHGDTLYAYTEVLETAEADREDAGIVTFRHWGTNQDDKVVFEGERTVMLKRKS
                     HYGAIPQ"
     gene            complement(2378..2872)
                     /locus_tag="AC16PP_04095"
     CDS             complement(2378..2872)
                     /locus_tag="AC16PP_04095"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039588.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6480837.1"
                     /translation="MSETNDFRDRAVLLSKGNRYEDMTEGRVFDHHWGRTITDGDNAA
                     FTTQTLSFCPLYFNEPYARSLGHPGTVVNPLLVFNTVFGLTVEDLSEGGGPFLGVDDC
                     EFLADVYVGDTLTARSTVVTARDSKGQPAFGIVTWHTEGFNQHGEKVLAYHRTNLVRK
                     RKRD"
     gene            complement(2869..>3678)
                     /locus_tag="AC16PP_04100"
     CDS             complement(2869..>3678)
                     /locus_tag="AC16PP_04100"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF040249.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR6480838.1"
                     /translation="RALGIAGTQAAGLKSQFGTMCKPLHAGHAAATGVQSASLAQRGF
                     DSRRDILEVEQGFMATQASGACEEGFARAMERDAYTQDICFKYHAACYLTHSSIEAVN
                     NLCRRNAFDPNDIESIEIRVDAGHFRVCNILKPRSGLEAKFSLRFTAAMALAGVDTAS
                     IEIFTDALTQQPELVAFRDKVRVVAHETPNPDSIVTITTRDGSTHREAVNVAIPERDL
                     DAQWEKLEHKFHALVDDRVGRAHADELAGLVRTLDSQDDLGDFFSLLRVHR"
BASE COUNT          559 a         1198 c         1210 g          711 t
ORIGIN      
        1 ctccgcggtc tcgccggcat ctagcagcgt ggcagcgtgg tacatcagct gccgggccgc
       61 ctcgatctcg ctggccatgc gggcgatctt gaagcgggtt tcctgaaagt cggcgatggg
      121 cacgccgaac tgcacccgct cgtgggagta ggccatggcg tcctcgagcg cccctcgcgc
      181 gagcccgatg gagcgggcgg cggtgtgggc ccgggcggcg ttcagaaagt gcatggcgtt
      241 cttgaaaccg tcaccgccac cgccggtgat gatgttttcc ttggggatcc ggcagccgtc
      301 gaaggccagc tcccacgtct tccacccgaa gtagccgatc ttgggtatgg gtgatccgga
      361 gcagccctcg ggcagctccc cccggggttt ctcgatgatg aagttcttca gccccttgtg
      421 ctgggcatcg tccgagtcgg gggcttcgac ccgggcgaga agctggatgt agtccgcacc
      481 gtcggcgaac gtgcaccagt acttgctgcc ggtgatgacc cactcgtccc catccagcac
      541 ggcccggcac ttggccgcag ccagatcgga gccgacgtcc ggctccgaaa gggagatcgc
      601 ggccagatac tcgccccgcg cggccttctg cgtcagctcc tcgcgcttct caaggggcca
      661 gccgcccacg ttcatgagcg acgtggcccg ggcgatgatg ctcgccacgc tcatccagcc
      721 ccgcgccagc tcctcgctga tcaggcagta ctcgaacacg ccgagcccca ggccgccgtg
      781 ctcctcggcc atgcggatgc cgaaatagcc catctcgccg agcttgtcgc gcaggtccat
      841 ggggatgtcc ccctggatgg ggtcgagctc gttggccacg ggcagcactt ctcgccgggt
      901 gaagtcccgg gcgacctcct gaatcatgcg tcgctcgtcg gtgaggtaag tctcggtcat
      961 tggctacatc ccaatcagtt ctgcccgatc gagcaggttc tgcacgattc ggatactggc
     1021 ggcgtccacc atcacgccgt ccacgttgat ggctcccagc ccctggctca aggcctcgtt
     1081 gtaagccgct ttctgtttgc gggcgtcggc gatggctgcc gggtccggtg tgaaaaccgc
     1141 gttggcgagc tcgatctggc tcggatggat ggcccacttg ccgaccatgc cgagcacgaa
     1201 ggcgcgctcg gcctccttgc tgtacgcctc cggattgcga aagttcgcga agggcccgtc
     1261 caccgggtcg ataccgttgg cgtggcaggc gatggtcatc ttgtagcgcg ggtagtgcca
     1321 gacgtctggc gggtagcctc ccgagctgcc gatgtggccg agctgcatct gctggctggc
     1381 ggagtagtcg cccatcccga acacgaggca ctcgagccga tcggagcagg cggcaatctc
     1441 ctccacgtgc atcatcccct ccacctcttc gatgaggcac tcgatgccga tgcggcgcgt
     1501 aagccccagc ttctgctcca gctggtcgag catcaggtgg acgaacatga cgtccgccgc
     1561 gctcttggct ttggtcagca tgatcgtgtc gagcttttcc ccggctccgg tgaccacctc
     1621 gatgatgtcg tcgtggcacc actgggtctc cacgtcgttg atgcgcacgc agcgggtgcg
     1681 cggcgtccag tggagcccgt tcagcgcgtc cacgatcatg ccgcgggcgt cgggcttggc
     1741 gctgggggcc accgcgtcct cgaggtcgag gaccacgtga tccagatcca gggccgcggc
     1801 cttggccatc atcttctcgg agctgccggg tacggagagc tggcagcgcc tggcgcgttt
     1861 gggttgtcgg ttcattgcgg tatcgctccg tagtggctct tgcgtttgag catgacggta
     1921 cgttctccct cgaaaacgac tttgtcgtcc tggttggttc cccagtgcct gaaggtcacg
     1981 atgccggcat cctcccggtc ggcctccgcg gtttcgagca cctcggtgta ggcgtacagc
     2041 gtgtcgccat gaaaaacggg cgccttcagg cggatcttgt ccagcttcag ctcccgcaag
     2101 gcgttctcgc ccgtatcctg catggcgagg ccaatcacca tggcgatggt cacgccaccg
     2161 aactgcaggc gcgagttcca gccgccgcgg ccgtccccca gagccagcgc gttgcgctgc
     2221 atgacgtcct cgttgaagtg cccctcggcg gtgttgagca cctgcagggt gatcccgacg
     2281 ttgtcctgct cgcacatggt cttgccccgg gcgtgccgga aaacctggcc ggcctggaag
     2341 tcctcgaaat agttgcgctc gccggtcaac gccatggtca atcccgcttg cgcttgcgga
     2401 ccaggttggt ccggtgatag gccagcactt tctccccgtg ctggttgaag ccttcggtgt
     2461 gccaggtgac gatgccgaaa gccggttggc ccttcgaatc gcgcgcggtc accaccgtgc
     2521 tgcgcgcggt gagcgtgtcg cccacgtaca cgtccgccag aaattcgcag tcgtccacgc
     2581 ccaggaacgg accgcccccc tcgctcaaat cctccaccgt gagaccgaaa acggtgttga
     2641 agaccagcag gggattgacc acggtccccg ggtggccgag ggatcgggca tagggctcgt
     2701 tgaaatagag cgggcagaag gaaagggtct gggtggtaaa cgccgcgttg tccccatcgg
     2761 tgatcgtgcg cccccagtgg tggtcgaaca cccggccctc ggtcatgtcc tcgtagcggt
     2821 tacctttgct aagcagcacg gcccgatcgc ggaagtcgtt cgtttcgctc atcgatgaac
     2881 ccgcagcagg ctgaagaagt cgcccagatc gtcctgggag tccagggtcc tgaccaggcc
     2941 ggcgagctcg tccgcatggg cacggccgac gcggtcgtcc accagggcgt ggaatttgtg
     3001 ctctaacttc tcccattggg catccaggtc tcgctcaggg atcgccacgt tgacggcctc
     3061 tcggtgggtg ctcccgtccc gggtggtgat ggtgacgata ctgtccgggt tgggcgtctc
     3121 gtgggcgacc acgcggacct tgtcccggaa ggccaccagc tcgggttgct gagtcagcgc
     3181 gtcggtgaag atctcgatgc tcgccgtgtc gacgccggca agggccatgg ccgccgtgaa
     3241 gcgcaggctg aacttggcct ccaggccgga tcggggcttg aggatgttgc acacccgaaa
     3301 atgaccggca tccacccgga tctcgatgct ttcgatgtcg ttcgggtcga aggcgttgcg
     3361 ccggcagaga ttgttgaccg cctcgatgga ggagtgggtc aggtagcagg ccgcgtggta
     3421 cttgaagcag atgtcctggg tgtaggcgtc ccgctccatg gcccgggcaa agccctcctc
     3481 gcaggcgccg ctggcctggg tcgccatgaa gccctgctcc acctcgagga tgtcccgtcg
     3541 gctgtcgaag ccccgctgcg ccaggctggc tgattgaacg ccggtggcgg ccgcgtggcc
     3601 ggcgtgcagc ggtttgcaca tggtcccgaa ctgagatttc aacccagcgg cctgggtgcc
     3661 ggcgatgccg agggcgcg
//
LOCUS       DBNHKV010000130        14606 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_90607, whole genome shotgun sequence.
ACCESSION   DBNHKV010000130 DBNHKV010000000
VERSION     DBNHKV010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 14606)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14606)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14606
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_90607"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..330
                     /gene="rpmB"
                     /locus_tag="AC16PP_04105"
     CDS             94..330
                     /gene="rpmB"
                     /locus_tag="AC16PP_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR6480839.1"
                     /translation="MSRVCQVTGKRPITGNNVSHAHNKTRRRFSPNLHYHRFWVDSEK
                     RFVRLKVSSQGMRIIDKLGIDQVLADMRKRGEKV"
     gene            356..511
                     /gene="rpmG"
                     /locus_tag="AC16PP_04110"
     CDS             356..511
                     /gene="rpmG"
                     /locus_tag="AC16PP_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR6480840.1"
                     /translation="MRDKIKLVSSAGTGHYYTTDKNKRTTPDKLEMKKYDPVVRKHVV
                     YTEAKIK"
     gene            540..1412
                     /gene="mutM"
                     /locus_tag="AC16PP_04115"
     CDS             540..1412
                     /gene="mutM"
                     /locus_tag="AC16PP_04115"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HIR6480841.1"
                     /translation="MPELPEVETTRRGVAPLVDGRYLLDWNLRNPNLRWPVQLPDELR
                     GSRIARVRRRAKYLIFELEPSALGAAGSLIIHLGMSGNLRVLPQGSPYLPHDHVELAL
                     ASEGEGAATRVLRLNDPRRFGSLHWQPGEAEAHWLLAGLGPEPLSEAFSGEYLKRAAR
                     GRRVAVKNFVMDGRVVVGVGNIYANEALFLAGLRPTARAGRVTRLGYDRLVAAIKQVL
                     TEAIDMGGTTLRDFVNQDGNPGYFKQSLNVYGRLGLPCRRCATPLKGTRTGQRATVFC
                     PNCQGAQSFSGRNR"
     gene            1475..1786
                     /locus_tag="AC16PP_04120"
     CDS             1475..1786
                     /locus_tag="AC16PP_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480842.1"
                     /translation="MKRMKRAILAFTLALFMATPALAAPEDSPSAFTMTGDLVVARPL
                     GVAMTAIGAAVFVVSLPFSALGGNVGESADALVMGPVNETFVRCLGCPSAGRRQAPPA
                     N"
     gene            complement(1835..2308)
                     /gene="coaD"
                     /locus_tag="AC16PP_04125"
     CDS             complement(1835..2308)
                     /gene="coaD"
                     /locus_tag="AC16PP_04125"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR6480843.1"
                     /translation="MRTVVYPGSFNPITNGHTDLVKRALNLFDHVIVAIGTSAQKDPS
                     VALKNRIDLCRLVLAEFGDKVTVEGFNSLLVNYVRERNARFILRGLRTVTDFDYEFQM
                     AEMNQSLAPEIEYVFLPTAQNCSFISSTLVREIASLGGDISQFVHPAVVEAYHRD"
     gene            complement(2351..2902)
                     /gene="rsmD"
                     /locus_tag="AC16PP_04130"
     CDS             complement(2351..2902)
                     /gene="rsmD"
                     /locus_tag="AC16PP_04130"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR6480844.1"
                     /translation="MASNQVRIIGGKWRGRKLAFPDAATLRPTLGRVRETLFNWLAGR
                     IAGACCLDLYAGTGALGFEALSRGAAEADMVERARTAVAALRRNAPLLKAPARVHGIP
                     AERFLQATDRTWDVIFLDPPFADDALPRALEVIAERGLLRPGGCVYFEQAERGGTAPA
                     GPGWRLLKKSRAGASEFGLLVRD"
     gene            2979..3932
                     /gene="ftsY"
                     /locus_tag="AC16PP_04135"
     CDS             2979..3932
                     /gene="ftsY"
                     /locus_tag="AC16PP_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR6480845.1"
                     /translation="MSEQDQSSAEGGGLWSRLKRGLGQTRNQLAEGVGNLLLGEKEID
                     DEVLSELETALLLTDVGIDTTREIMDKLAVRVRRKELNDTVALHQALGSLLKELLLPV
                     EKPFTIAQDKRPFVIFMVGVNGAGKTTTIGKLAKRLRDDGLNVVLAAGDTFRAAAVEQ
                     LQTWGERIGVPVIAQGQGSDSASVVYDAVAAARARGHDVVLADTAGRLQAKANLMEEL
                     AKVKRVVNRMDPDAPHEVLLVLDAGVGQNALSQVREFDAAVGVTGLIFTKLDGTAKAG
                     VLFGIARQVMKPVYFVGVGEGADDLRPFDAKDFVDALLAPE"
     gene            3947..4639
                     /gene="ftsE"
                     /locus_tag="AC16PP_04140"
     CDS             3947..4639
                     /gene="ftsE"
                     /locus_tag="AC16PP_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR6480846.1"
                     /translation="MGQIEFHHVTKRFDDGFEALKDVSVDFPEGSMTFLTGHSGAGKS
                     TFLKLLLCADRPSAGQIIVNGVNVRHISARRLAHYRQHIGVVFQDHHLLHHRDVYENV
                     ALPLRVAGMRERAVARRVRASLTRVGLLAKERMFPRYLSTGEQQRVGIARAVATRPRI
                     LLADEPTGNLDPELSRDIMHVFEQVNQVGTTVIIASHDRDLIESMGLRIIELAHGNIV
                     RDTDREADIGFE"
     gene            4626..5642
                     /locus_tag="AC16PP_04145"
     CDS             4626..5642
                     /locus_tag="AC16PP_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HIR6480847.1"
                     /translation="MASNNEAQGGRPAPATGGGRGRTKKGYPWPHRRVGLVAWWQDHL
                     RTGRETLHFISARLGTSLLVWLLVGIALALPAGLILLQLNLAGMTADWDGRPGLSVYF
                     ELNTDAGKVAAIAQRLSERAEIEGVRVTSPEEALAEFQSHADVGDALDLLTENPLPAS
                     LRATLVPGSSLLDLELVAGLVEGAPGVSEVVVEKTWLERVIDISRVVSRLGIILGALF
                     GVGAVLVTATSVRLAIEARLEELRVLKLVGATDRQIRRPFMYFGALYGLGGGLVAAML
                     ISLCLVAIETPVSNLLGSYGRDLETPGFDPTFLSGLLLVGAFLGVSGALLAARQRLKD
                     LEIL"
     gene            5733..6596
                     /gene="rpoH"
                     /locus_tag="AC16PP_04150"
     CDS             5733..6596
                     /gene="rpoH"
                     /locus_tag="AC16PP_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HIR6480848.1"
                     /translation="MSTDVLSMKSLAPGRDLDAYVQTVSAFPVLSAEQEKDLAERFYY
                     DNDLEAARELVLCHLRFVVHLARSYRGYGLPQADLIQEGNVGLMKAVKRFNPEVGVRL
                     VSFAVHWIKAEMHEFILRNWRIVKVATTKAQRKLFFNLRSSKKRLAWLTQEETRAMAR
                     ELGVSEEEVARMEGRMAASDLAFDGYASDDDSSPLAAPVHYLEHEGSNPEDLVAEEDW
                     HDEASARLGAALAELDERSRAIVEARWLAEKKATLHELGDTYGVSAERIRQIEQNAIK
                     KLKAACMSDAA"
     gene            6619..6993
                     /locus_tag="AC16PP_04155"
     CDS             6619..6993
                     /locus_tag="AC16PP_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HIR6480849.1"
                     /translation="MPANVPLAAGALFGLTGVVMGALGAHGLAGQLSESGVDIWDTAV
                     LYHLTHALALVAVGVRLGQKVPPPWPVVLAGWGFGVGILLFSGSLYVLALGGPPVFGP
                     LTPLGGLALCAGWVALLAAARR"
     gene            7013..7693
                     /gene="trmB"
                     /locus_tag="AC16PP_04160"
     CDS             7013..7693
                     /gene="trmB"
                     /locus_tag="AC16PP_04160"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF014447.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIR6480850.1"
                     /translation="MEERTYLRRRGRMTRGQARALDRAGDTMIVDSTSPDWPAVFGRQ
                     APLGVEVGFGMGHALLDWAAECPDWNLVGIEVYQPGIGALLLGLDSQGSTNVRVLEAD
                     ARQALAAGFEPDSIDEVRILFPDPWPKKRHHKRRLVQPDFVRLLASRMRPGARLGLAT
                     DWQDYAQHMLTVLDAEPLLQNRSGQGSYAERPSGRPTTRFEERGRRLGHQVWDLGYER
                     KRETTESR"
     gene            complement(7657..8760)
                     /gene="hemW"
                     /locus_tag="AC16PP_04165"
     CDS             complement(7657..8760)
                     /gene="hemW"
                     /locus_tag="AC16PP_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR6480851.1"
                     /translation="MGPVGLYAHFPWCVRKCPYCDFNSHPVRGSIDEAGYVSALLADL
                     RKNLPRDVPIGSVFFGGGTPSLFHAPAFAAVLEEVASQLTEDAEVTMEANPGTTEHSD
                     LRAYRQAGINRLSIGAQSFDDEALERLGRIHHAAETLDCAARARSAGFENINIDLMYG
                     LPHQTPDQAVQDLEAALALEPDHISWYQLTIEPRTEFARRPPPIPPEDRIDETEQRGR
                     DRLEQAGFERYEVSAYARPGRRSRHNVNCWSFGDYVGIGAGAHGKLTSGGRVARTVKP
                     SQPRLYLQDPESQQRQIVEAEALPFEFMLNALRLRDGVPLDQFTQRTGLALEALDPVW
                     GELVDEGLVHRHRLATTPEGYRYLDSVVSRFLS"
     gene            complement(8760..9347)
                     /gene="rdgB"
                     /locus_tag="AC16PP_04170"
     CDS             complement(8760..9347)
                     /gene="rdgB"
                     /locus_tag="AC16PP_04170"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR6480852.1"
                     /translation="MSIVLASNNPGKLAELRLLIAPLGLDAVSQRDLGIASAPETRAT
                     FIENALEKARHVSAAAGMPAIADDSGLAVPALGGAPGVRSARYAGEDATDAENNAKLV
                     AAMADMDDRRASFHCAVVFMRAPDDPVPVVATAAWSGRIVADPRGTGGFGYDPHFFID
                     ETDCTAAELDPSVKNEISHRGRAMRALCAALAGVV"
     gene            complement(9355..9792)
                     /locus_tag="AC16PP_04175"
     CDS             complement(9355..9792)
                     /locus_tag="AC16PP_04175"
                     /inference="COORDINATES: protein motif:HMM:NF025822.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4426 domain-containing protein"
                     /protein_id="HIR6480853.1"
                     /translation="MVKTGAGWICALILSWAGTPGHAEQRQSFGGWDVHYVVLPTGFL
                     KPAIAAQYGITRGRDRALVNISVLDPTGAPQKVSIEGTVKNLLGQLQPLAFREVTEGQ
                     AVYYLAAVKHTHEEVLRFEISLSAPDAPPMQLKFQQKLYWEDT"
     gene            complement(9792..10400)
                     /gene="metW"
                     /locus_tag="AC16PP_04180"
     CDS             complement(9792..10400)
                     /gene="metW"
                     /locus_tag="AC16PP_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008364885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HIR6480854.1"
                     /translation="MRPDLEIIAEVIKPGARVLDLGCGEGELLAYLQTSKQVNGYGLE
                     IDPDNITTCLHNGVNVIEQDLDEGLDNFPDDSFDLVVMTETLQAVQNPDRMLDDMLRI
                     GGECIVTFPNFGYWRCRAYLALRGRMPVSEHLPHAWYDTPNIHLCTFKDFERLCHSKG
                     LPVIDRFVVDERHLARRLMQRFPNFFGHIAFYRLGRPNSGEA"
     gene            complement(10390..11544)
                     /gene="metX"
                     /locus_tag="AC16PP_04185"
     CDS             complement(10390..11544)
                     /gene="metX"
                     /locus_tag="AC16PP_04185"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase MetX"
                     /protein_id="HIR6480855.1"
                     /translation="MSSSLPSDSVGLVSPQVFAHPSGFSLRCGETLEGFELVYETYGR
                     LNAARSNAILVCHALSGSHHAAGYHSEDDKRPGWWESCIGPGKPIDTDRFFVVCPNNL
                     GGCHGSTGPTTVDPRTGEPFGPNFPVVTVEDWVDTQALLADHLGIDRFAAVVGGSLGG
                     MQAMRWATDYPDRLHAAVLIACAARLSAQNIAFNEIARQAIISDPEFHGGNYRQLGVN
                     PDLGLMLARMVGHVTYLSDDGMRQRFGRQLKSGDIETAQDVEFQVESYLHHQGRSFSQ
                     RFDANSYVLMTRALDYFDPAREHDDDLVKALASATCRFCVISFTTDWRFSVARSKELV
                     DALIAAGKNVASAIIESEHGHDSFLLNVPRYFDVLRAYLGRIATEAVDAP"
     gene            complement(11645..12181)
                     /locus_tag="AC16PP_04190"
     CDS             complement(11645..12181)
                     /locus_tag="AC16PP_04190"
                     /inference="COORDINATES: protein motif:HMM:NF014389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR6480856.1"
                     /translation="MAEVLYYATSFILQVIAFLFVVRFLLQACRADFYNPISQGIVKA
                     TDVVLKPVRMVLPGYRNLDFAALLAALIVEVVMILALGAIADRPTGTAVQIATSGVLQ
                     MILLGLFIIKWSILIVIIASFLAPGNYHPALALLQQLTEPVLAPARKLLPPMGGLDFS
                     PILVFLILGVIERVLLQI"
     gene            complement(12212..13021)
                     /gene="proC"
                     /locus_tag="AC16PP_04195"
     CDS             complement(12212..13021)
                     /gene="proC"
                     /locus_tag="AC16PP_04195"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR6480857.1"
                     /translation="MSSHITFIGAGNMATSLIKGLIASGHDAATIRASDPAPEQLGKI
                     AELGVATSDDNNAQVRGADVVILAVKPQVMASVAGGLTALSTDQLLVSIAAGVPMSSL
                     AGWTSSEQPIVRCMPNTPALLGAGVTALCANERVSPAQRELAESVLATAGRTLWVSLE
                     DQLDAVTAVSGSGPAYFFYLMEAMIEAGQALGLSAETAVTLTLETARGAALMALEGPP
                     PAQLRANVTSPGGTTQAALEILDAADCRGIINAAVAAAAERSRELAIEFGR"
     gene            complement(13055..13732)
                     /locus_tag="AC16PP_04200"
     CDS             complement(13055..13732)
                     /locus_tag="AC16PP_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR6480858.1"
                     /translation="MHSISHNVASVRSCVVSAARAAQRDPDEVCLIAVSKRQPARAIE
                     EAHGAGLKDFGENYLQEALPKIAALGHLPLTWHFIGALQSNKTRQVARHFDWVHTVDR
                     PKIARRLNEQCPEDKTLQVCLQVNIDADPGKAGVDPAAVADLLKELVDLERICVRGLM
                     TILDPNADAPESFARMAKLFAGLAPLAAGRWDTLSMGMSNDYEQAIMAGATHVRIGTA
                     IFGAREG"
     gene            13898..>14606
                     /locus_tag="AC16PP_04205"
     CDS             13898..>14606
                     /locus_tag="AC16PP_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR6480859.1"
                     /translation="MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEH
                     QQVHSLIYEIMNDKQRKDFEEFLETDFSVEVPGVARFRVNAFNQNRGAGAVFRTIPSK
                     VLTMEDLDMGQVFRDICLVPRGLVLVTGPTGSGKSTSLAAMVDFINDHRYDHILTIED
                     PIEFVHESKKCLVNQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAA
                     ETGHLVFGTLHTTSAAKT"
BASE COUNT         2572 a         4518 c         4932 g         2584 t
ORIGIN      
        1 ccgagcgagg cctgctctga acgcggccgg ctgttgccgg ccgaagggcg ctctggtata
       61 aacgcgcctc tttttcgcca ggaaaacggg accatgtcgc gcgtttgtca ggtaacgggt
      121 aagcggccga tcaccggcaa caacgtcagt catgcgcaca acaagacgcg ccggcgtttt
      181 tcgcctaacc tgcattacca tcgcttctgg gtggacagcg agaagcgctt cgtgcggctg
      241 aaggtttcgt cccagggcat gcgcataatc gataagctgg gcatcgatca ggtccttgcc
      301 gacatgcgca agcgcggcga gaaggtctga gtgggctaac ggcaggtaaa gacagatgcg
      361 cgacaagata aagctggttt ccagtgcggg caccggacac tactacacca ccgataagaa
      421 caagcgtacg acgccggaca agctggagat gaagaagtac gatcccgtcg tccgcaagca
      481 cgtcgtctat accgaagcga agatcaagta accggcggat cagcgccccc tgctgatcgg
      541 tgcccgaact acctgaagtc gagaccaccc gccgcggcgt agcacccctg gtcgacgggc
      601 gctacctgct cgactggaat ctgcgcaacc ccaatctgcg ctggccggtg cagctacccg
      661 atgagctgcg cggttcccgg atagccagag tcaggcgccg ggccaagtac ctcatattcg
      721 agctcgagcc atcggccctg ggcgctgcgg gcagtctcat catccacctg ggcatgtctg
      781 gaaacctgcg ggttctgccc cagggctcgc cctacctgcc ccacgatcac gtggagttgg
      841 ccctcgcatc ggagggcgag ggggccgcga cgcgcgtgct tcggctgaat gacccgcgtc
      901 ggttcggcag cctgcactgg cagccgggtg aggccgaagc ccactggctc ctggcggggc
      961 tcggcccgga gccgctctct gaggccttct cgggagagta tctgaagcgg gcggccagag
     1021 gccgccgggt tgccgtcaag aactttgtca tggacggcag ggtggtggtg ggcgtcggga
     1081 acatctatgc caacgaggca ctgttcctgg ccggcctgcg gcctacggca cgggcaggcc
     1141 gtgtcaccag gcttggctac gatcggctgg tggcggccat caagcaggtg ctgacggaag
     1201 ccatcgacat gggcggcacc acattgagag attttgtcaa ccaggatggc aatccgggtt
     1261 atttcaagca gtcgctcaac gtctatggcc ggctcggatt gccctgccgt cgctgcgcga
     1321 cgccgctcaa agggacccgc accgggcagc gagctaccgt tttttgtcca aattgccaag
     1381 gcgcccaaag ttttagcgga cgcaatcgct aaagtaggcg cggtgcgctc ggcaccagga
     1441 aaagggtcga gggcggttga ctagcaaaaa ggacatgaag cgaatgaaac gcgcaattct
     1501 ggcattcacc ctggccctgt tcatggccac tccggcactt gctgcgccgg aggactcacc
     1561 ctctgcattc acgatgacgg gcgatcttgt cgtcgccagg ccccttggcg tcgccatgac
     1621 ggcaatcggc gccgcagtat tcgtcgtcag cttgccgttc tcggcacttg gcggaaacgt
     1681 cggcgagtcc gcggacgcgc tggtcatggg ccccgtcaac gaaacgtttg tgcgttgcct
     1741 cggttgcccc agcgccggtc gccgccaggc gccgcccgcc aactgatttc cccctgatgc
     1801 cggggctctt cgataggggc cccggtgctg gcggctagtc ccggtggtag gcctcgacca
     1861 ccgcgggatg gacgaattgg gagatgtctc cgcccaggga ggcgatctcg cgcaccagcg
     1921 tggacgaaat gaaagagcag ttctgcgctg tgggcagaaa cacgtactcg atctccggtg
     1981 ctagcgactg gttcatctcc gccatctgaa actcgtagtc gaagtcggtc accgtgcgca
     2041 ggccgcgcag aatgaaccgc gcgttgcgtt cccgcacgta gttcaccagc agggaattga
     2101 atccttccac ggtgactttg tcgccgaact ccgccagcac caagcggcag agatcgatcc
     2161 ggttcttcag cgccactgaa ggatctttct gtgccgacgt gccgatggcc acgatgacat
     2221 ggtcgaagag gttgagcgcc cgcttgacca gatccgtgtg tccgttagtg atcgggttga
     2281 atgaacccgg gtatacgacc gtgcgcattg tccccacccc actgagaagc ccgcgatggt
     2341 atcggggcgg tcaatccctg acaagcaggc cgaactcgct ggcgccggcg cggctcttct
     2401 tcagcaggcg ccagccaggg ccggcgggcg ccgtgccccc cctttcggcc tgctcgaagt
     2461 aaacgcagcc gccgggccga agcaggccgc gctcggcgat cacctctagg gcgcggggca
     2521 gcgcatcgtc ggcgaatggc ggatccaaga agatcacgtc ccaggtgcgg tccgtggctt
     2581 gcaggaaacg ttcggctgga atgccgtgaa cgcgggctgg cgctttcagc agcggcgcgt
     2641 tgcgtcgcag cgcggccacg gcggtacgcg ccctctccac catgtctgcc tccgcggcgc
     2701 cgcgggacag cgcttcgaaa cccagcgcgc cggtgccggc gtagagatcg aggcagcagg
     2761 cgccggcgat ccggcccgca agccagttga acagagtttc gcggacgcga cccagggtgg
     2821 gcctgagcgt ggcggcatcc gggaaggcga gctttcgtcc cctccactta ccgccgataa
     2881 tacgcacctg gttgctggcc atccgaagga tggtgagcga accgtgccag tcagacaagc
     2941 cgtagaccgg cgagcgcatc gccgggaaaa ggatcatcgt gagcgagcag gatcagtcga
     3001 gtgcggaggg aggagggctg tggagccggc tgaagcgggg gctcggccag acgcgcaacc
     3061 agctggccga aggcgtcggc aatctgcttc tcggcgagaa ggagatcgac gacgaagttc
     3121 tctcggagct ggagaccgcg ttgctgctca ccgacgtcgg catcgacacc acccgggaga
     3181 tcatggacaa gctcgccgtg cgcgtccgcc gcaaggagct caacgacacc gtcgcccttc
     3241 accaggcatt ggggagtctg ctcaaggagc ttttactgcc cgtcgagaag cccttcacca
     3301 ttgcacagga caaacgtccg tttgtgattt tcatggtggg cgtcaacggc gcgggaaaga
     3361 cgacgaccat cggcaagctg gccaaacgac ttcgcgacga cggcctgaac gtggtgctgg
     3421 ccgcgggcga cacctttcgc gctgcggccg tggagcagct tcagacctgg ggcgagcgga
     3481 ttggcgtgcc ggtcatcgcc cagggtcagg ggtccgacag cgcctcggtg gtctatgacg
     3541 ccgtggcggc ggcccgggct cgcggccacg acgtcgtgct ggcggacacc gcggggcgcc
     3601 tgcaggcgaa ggccaatctc atggaggaac tcgccaaggt gaagcgggtc gtgaatcgca
     3661 tggacccgga cgctccccac gaggtcctgc tcgtgctcga tgccggcgtg gggcagaacg
     3721 ccctcagcca ggtccgcgag ttcgacgcgg ccgttggggt gacggggctg atcttcacga
     3781 agctggacgg aacggccaag gccggggtgc tgttcgggat cgcgcgtcag gtcatgaagc
     3841 ccgtctactt cgtcggcgtg ggcgagggtg cggacgacct caggcccttc gatgccaaag
     3901 atttcgtcga cgcccttctc gcgcccgagt aagcaaagcg ggcaccatgg gacagatcga
     3961 gtttcatcac gtcaccaaac gattcgacga cggcttcgaa gcgctcaagg acgtcagcgt
     4021 cgactttccc gaaggctcca tgacctttct gaccgggcac tccggggccg gcaagagcac
     4081 gtttctgaag cttttgctgt gtgccgatcg cccgtcggct gggcagatca tagtcaacgg
     4141 agtgaacgtc cggcacatca gcgccaggcg tctcgctcac taccgacagc acatcggcgt
     4201 ggttttccag gatcatcacc tgctgcatca tcgtgacgtg tacgagaacg tagcgctgcc
     4261 gttgagggtc gccggaatgc gcgagagggc agtggctcgg cgggtcaggg cgtcgctgac
     4321 ccgggtcggg ctgctggcga aggagcggat gtttccgcgt tacctttcca ccggtgagca
     4381 gcagcgggtt ggcattgccc gggcggtggc cacacggccg aggatccttc tggcggacga
     4441 gcccacgggc aatctggatc ccgagctctc ccgggacatc atgcacgtct tcgagcaggt
     4501 caatcaggtc ggcaccacgg tgatcatcgc gagccacgat cgagacctga tcgagtccat
     4561 ggggctgcgc atcattgagc ttgcccacgg caacatcgtc agagatacgg acagagaagc
     4621 ggacattggc ttcgaataat gaggcgcaag ggggtcggcc cgcgccggcc acgggcggcg
     4681 ggcgaggccg cacgaagaag ggatatccct ggccccaccg ccgcgtgggg ctggtggcct
     4741 ggtggcagga ccatctgcgc acgggacggg agacgctgca tttcatcagc gcccgtctgg
     4801 gaacgagcct gctggtctgg ttgctggtgg ggatcgcgct ggctctgccc gcggggctga
     4861 ttctgcttca gctcaacctc gccggcatga cggccgattg ggatgggcga ccggggctgt
     4921 ctgtgtattt cgagctgaac accgacgcag gaaaggttgc ggcgattgcc caacggctgt
     4981 ccgaacgtgc cgagatcgag ggtgtcaggg tgacctcgcc cgaggaagcg ctggctgaat
     5041 ttcaaagtca cgcggacgtt ggagacgctc tcgacctgct gacggagaac ccgttgccgg
     5101 cctcgctgcg cgccaccctg gtgccggggt cttcgctgct cgatctggag ctggtcgcgg
     5161 ggcttgtcga gggagcgccg ggagtatcgg aggtggtggt cgagaagacc tggctggagc
     5221 gggtcatcga catttcccgg gtggtcagcc gcctggggat cattcttggg gcgcttttcg
     5281 gtgtcggcgc ggtgctggtc accgcaactt ccgtgcggct ggccatcgag gcgcggctgg
     5341 aggagctcag agtgctgaaa ctggttgggg ccaccgaccg acagatccgt cggcccttta
     5401 tgtacttcgg cgcgctttac gggcttggtg gcggtctcgt ggccgccatg ctgatcagcc
     5461 tttgcctggt tgccatagag acaccggtct cgaatctcct cggaagctac ggccgtgacc
     5521 ttgaaacgcc ggggttcgac ccgaccttcc tttccgggct gctgctggtc ggcgctttcc
     5581 tgggagtgtc cggggcgctt ctggctgcgc ggcagcgtct gaaggatctc gaaatccttt
     5641 agttttcggc gggttaggga accattcgcg gggttagcag tctcaccacc agagtgctaa
     5701 actgctgaaa ttagtgttac cggaggtatc acatgagcac cgacgtgctt tctatgaaaa
     5761 gtctcgcccc gggacgcgat ctggatgctt acgttcagac ggtcagcgcg tttccggttc
     5821 tttctgcaga acaggagaag gatctggcgg agcggtttta ctatgacaac gacctggaag
     5881 ccgcccggga actggtgctc tgccatctgc gcttcgtcgt ccacctggcc cgttcgtaca
     5941 gaggctacgg gctgcctcag gcggacctga tccaggaggg caacgttggc ctgatgaagg
     6001 ccgtcaagcg gttcaatccc gaggtgggcg ttcgcctggt ctctttcgcc gtgcattgga
     6061 tcaaggcgga aatgcacgag ttcatcctgc gtaactggcg gatcgtcaag gttgctacca
     6121 ccaaggccca gcgaaagctc ttcttcaacc tgcgcagctc caagaagcgg ctcgcctggc
     6181 tcacccagga agagacccgt gccatggccc gggagctggg cgtttccgag gaagaggtcg
     6241 cgaggatgga gggccggatg gcagcgtccg acctggcctt cgacggctat gcttcggacg
     6301 acgactcctc gcccctggcc gcccccgtcc actatctgga acacgaaggt tcgaatcccg
     6361 aggacctggt ggcagaggaa gactggcacg acgaagccag tgcgcgtctg ggcgccgcgc
     6421 tggccgagct ggatgagcga agccgggcaa tcgtggaagc ccgatggctc gccgagaaga
     6481 aggcgacgct ccacgagttg ggcgacacat acggcgtgtc ggcggagcgg atccgccaga
     6541 tcgagcagaa tgcgatcaag aagctgaaag ccgcgtgtat gagcgacgcc gcctgatccg
     6601 ctgggcatcg ggggtacgat gccggccaac gttcctttag ccgcgggagc cctcttcggc
     6661 ctgaccggcg tagtgatggg cgcccttggc gcccacgggc ttgccgggca actgtctgaa
     6721 agcggcgtcg atatctggga caccgccgtg ctctaccatc tcacgcacgc cctagccctc
     6781 gtcgccgtgg gcgtgcgcct gggccagaag gtgccgccgc cgtggccggt cgttctggcc
     6841 ggctgggggt ttggtgtcgg catcctgcta ttctccggca gcctttacgt gctcgctctg
     6901 ggcgggccac ccgtcttcgg ccctctgacg cccctgggtg gcctcgcgct ctgtgccggc
     6961 tgggtcgccc tgctcgccgc cgcccgccgc tgagcgccgc tcgggacacg cattggaaga
     7021 aaggacttac ctgcgccgcc gcgggcggat gactcggggt caggctcggg ccctcgaccg
     7081 agcgggcgac acgatgatcg tcgactcgac atcgcccgac tggcctgcgg tgttcggccg
     7141 gcaagccccg ctgggggtgg aggtcggctt cgggatggga catgccctgc tggactgggc
     7201 ggccgagtgc ccggattgga acctggtggg gatcgaggtc tatcagccgg ggataggcgc
     7261 tctgctgctg ggcctggact cccagggctc gaccaacgtt cgcgtgctgg aagcggacgc
     7321 gaggcaggcg ctggctgcgg gctttgagcc cgactcaatc gacgaggtgc ggatactctt
     7381 tccggatccc tggcccaaga aacgtcacca caagcgccgg ttggtgcagc ccgatttcgt
     7441 ccgtctcctt gcgtcgagaa tgcggcctgg ggctcggctc ggcttggcta ccgactggca
     7501 ggactatgcc cagcacatgc tgaccgttct cgatgcggag ccgttgctgc agaaccgatc
     7561 ggggcagggt tcctatgccg agcgcccttc gggtcgcccg accacgcgct tcgaggagcg
     7621 tggccggcgg ctcgggcatc aggtgtggga tcttggctac gaaagaaagc gcgagaccac
     7681 cgagtccagg tagcgatagc cctcgggggt ggttgcgagg cgatggcggt gaacgaggcc
     7741 ttcatccact agctcgcccc agaccggatc gagagcctcc agggcaagcc ccgtgcgctg
     7801 ggtgaactgg tccaggggga caccatcgcg gaggcgaagc gcgttgagca tgaactcgaa
     7861 gggcagagct tcggcttcaa cgatctgccg ctgctggctt tcggggtcct gcaggtaaag
     7921 gcgcggctgg ctgggcttta ccgtgcgggc cacccgacca ccgcttgtca gcttgccgtg
     7981 ggcgcccgct ccgatcccga cgtagtcgcc gaacgaccag cagttcacgt tgtgacgcga
     8041 tcgccgacct ggcctggcgt acgctgagac ctcgtagcgc tcgaagcctg cctgctcgag
     8101 ccgatcacga ccccgttgct ccgtctcgtc gattcggtcc tccgggggaa tcgggggggg
     8161 tcgccttgcg aactcggtgc gcggctcgat ggtgagctga taccagctga tgtgatccgg
     8221 ttccagggcc agggcggcct cgaggtcctg aactgcctgg tccggagtct ggtgcgggag
     8281 accgtacatc aggtcgatgt tgatgttctc gaaaccggcc gatctggcgc gcgcggcgca
     8341 atccagggtt tcggcggcat gatggatgcg cccaagcctc tcgagcgcct cgtcgtcgaa
     8401 cgactgcgct ccgatggaca ggcggttgat gcccgcctgc cgataggcgc gcaggtctga
     8461 atgctcggtg gttccggggt tggcttccat ggtcacctcg gcgtcctccg tcagctggga
     8521 ggcgacctcc tcgaggacgg cggcgaacgc cggggcatgg aacaggctgg gcgtcccacc
     8581 gccgaagaag acgctgccga tgggcacgtc gcgcggcagg ttctttcgca ggtcggcaag
     8641 cagcgcgctc acgtagccgg cctcgtcgat agaccctcga acggggtgag aattgaaatc
     8701 gcagtacggg cacttgcgca cgcaccaggg aaagtgggcg tacaacccga ccggccccat
     8761 caaacgactc cggccagcgc ggcgcacagc gcgcgcatgg cccgaccgcg gtggctgatc
     8821 tcgttcttca cggacgggtc cagctcggcg gccgtgcaat ccgtctcgtc gatgaagaaa
     8881 tgcggatcgt aaccgaaccc gcccgtgccc ctgggatcgg caacgatgcg accgctccag
     8941 gcggccgtgg cgacgacggg tacagggtcg tcgggtgcgc gcatgaagac cacggcgcag
     9001 tgaaagcttg cgcggcggtc gtccatgtcg gccatggcgg cgaccagctt ggcgttgttc
     9061 tccgcatctg tggcatcttc gcccgcatag cgggcggagc gcacgccggg cgccccgccc
     9121 agcgccggca cggcgagtcc ggagtcgtcg gcgatcgccg gcatgcctgc cgcggcgctg
     9181 acgtggcggg ctttctccag cgcgttctcg atgaacgtgg cccgtgtttc cggtgcgctc
     9241 gcaatgccga ggtcgcgctg gctgaccgca tccaacccca gcggggcgat caggagccgc
     9301 aactcggcaa gcttgccggg gttgttgctg gcgagaacga tgctcaccgc gggatcacgt
     9361 gtcttcccag tacagcttct gctggaactt gagctgcatg ggcggcgcgt cgggagcgct
     9421 aagggaaatc tcgaaccgga gcacctcctc gtgggtgtgc ttgacggccg ccagatagta
     9481 gacggcctgg ccctcggtga cctcccggaa cgccagcggc tgcagctgac ccaacagatt
     9541 cttcacggtg ccttcgatgg agaccttctg gggagcgccc gtcgggtcga gcacggaaat
     9601 attgaccagg gcgcggtcgc ggccccgagt gatcccgtat tgcgcggcga tggccggttt
     9661 caggaagccg gtcggcagca ccacgtagtg cacgtcccag ccaccgaagg actgccgctg
     9721 ctcggcgtgt cccggcgtcc ccgcccacga caggatgagc gcgcagatcc agccagcgcc
     9781 cgtcttcacc atcaggcctc tcccgaattc ggtctgccca gcctatagaa ggcgatgtga
     9841 ccgaagaagt tgggaaatcg ctgcatcagc cgtcttgcga gatgccgctc gtcgacgacg
     9901 aagcgatcga tgaccggtag gcctttgctg tggcacagcc gttcgaaatc cttgaaggtg
     9961 cacaggtgga tgttgggcgt gtcgtaccag gcgtgaggca ggtgctcgga gacgggcata
    10021 cggccgcgca gcgccagata cgcgcggcaa cgccagtagc cgaaattcgg aaacgtgacg
    10081 atgcactcgc cgccgatccg gagcatgtcg tcgagcatgc ggtcggggtt ctgcaccgcc
    10141 tgcagcgtct cggtcatcac caccaggtcg aagctgtcat ccggaaaatt gtcgagcccc
    10201 tcatcgagat cctgctcgat gacgttgacg ccgttgtgca ggcaggtggt gatgttgtcg
    10261 ggatcgattt ccagcccgta gccgttgacc tgctttgaag tctgaagata agcgagcagc
    10321 tcgccctcgc cgcaaccgag atcgagcacc cgggctccag gtttgatcac ctccgcgatg
    10381 atctccaggt cagggcgcat cgacggcctc cgtggcgatg cggcccagat aggcccgaag
    10441 cacgtcgaag tatcgcggca cgttcaggag aaacgagtcg tggccgtgct ccgactcgat
    10501 gatggcgctg gccacgttct tgcccgccgc gatgagcgcg tccaccagct ccttcgagcg
    10561 cgccacggag aagcgccagt ccgtggtgaa cgagatgacg cagaagcggc aggtggcgga
    10621 tgccagcgct ttgaccaggt cgtcgtcgtg ctcccgggcc gggtcgaagt agtccagtgc
    10681 cctggtcatc agcacgtagc tgttggcgtc gaagcgctga gagaacgagc ggccctggtg
    10741 gtgcagatag ctctccacct gaaactccac gtcctgggcg gtttcgatat cccctgactt
    10801 cagctgccgg ccgaagcgct ggcgcatgcc atcgtcggac agataggtga cgtgacctac
    10861 catgcgcgcg agcatgaggc ccaggtccgg gttgacgccc agctgccggt agttgccgcc
    10921 gtgaaactcc ggatccgaga tgatggcctg ccgtgcgatc tcgttgaaag cgatgttctg
    10981 agcggacagc ctggcggcgc aggcgatcag cacggcggcg tgaaggcgat cgggatagtc
    11041 ggttgcccag cgcatggcct gcatgccccc caggctgcca ccgacgacgg cggcgaagcg
    11101 atcgatgccc aggtggtcgg cgagcagggc ctgagtatct acccagtctt caacggtgac
    11161 gacgggaaag tttggcccga acggttcacc cgtgcggggg tcgaccgtgg tcggccccgt
    11221 gctgccgtgg cagccgccga gattgttggg gcagacgacg aagaaccggt ctgtgtcgat
    11281 tggcttgccg ggcccgatgc agctttccca ccacccgggt cgtttgtcgt cctcggagtg
    11341 gtagccggca gcgtgatggc tcccggagag agcatggcag acgagaatcg cgttgctccg
    11401 cgcagcgttg agccgtccgt aggtctcgta gacgagctcg aacccctcga gcgtctcacc
    11461 gcagcgaagc gaaaagccgg agggatgcgc gaagacctgc gggctgacca ggcccacgga
    11521 atcggatggc aagctgctcg acactgagta ggccacgaag aacgggacgg cgagtctaaa
    11581 gcaagggctt caaggccatc aaccgggtcg gtcggggcat ccggccgggg cgcagccgat
    11641 tagctcagat ctgcagcagc acccgttcga tgacgccgag gatcaggaaa acgaggatcg
    11701 gcgagaagtc gagaccgccc atgggtggca ggagcttgcg ggccggtgcc aggacgggct
    11761 ctgtgagctg ctggagcagg gccagcgccg ggtggtagtt acccggagcg aggaagctcg
    11821 cgatgatcac gatcagaatc gaccacttga tgatgaacag ccccagcaga atcatctgca
    11881 gcacccctga ggttgcgatc tgcacagcgg ttcccgtggg ccgatcggcg atggccccca
    11941 gggcgaggat catcacgacc tccacgatga gcgctgccag gagggcggcg aaatccagat
    12001 ttcggtatcc gggcagcacc atccggaccg gcttcaacac cacgtcggtg gctttgacga
    12061 tcccttgaga aatggggttg tagaagtcgg cccggcatgc ctgcagcagg aaacgaacca
    12121 cgaacaggaa ggcaatgacc tgcagaatga agctggttgc gtaatataag acctctgcca
    12181 tgctcgttgc ctattcccct gattacgacg tttagcgacc gaactcgatg gcgagctcgc
    12241 gggatcgttc cgcggcggcc gctacagcgg cgttgatgat accccggcag tcggcggcat
    12301 ccagtatttc cagcgccgcc tgggtcgtcc cgcccggaga ggtcacattt gccctgagct
    12361 gcgcgggtgg gggcccttcg agagccatca gcgcggcgcc gcgggcggtt tccagcgtca
    12421 gggtgacggc ggtctcggcg ctgagcccca gggcctggcc ggcttcgatc atcgcttcca
    12481 tcaggtaaaa gaagtaggcg ggcccgctgc cggagacggc ggtcaccgcg tcgagctgat
    12541 cttccaagga tacccagagg gtcctgcccg cggtggccag cacgctttct gcgagctccc
    12601 tttgagccgg cgagacgcgc tcgttggcgc acagggcggt gactccggcg ccgagaaggg
    12661 ccggcgtgtt cggcatgcag cggacgatgg gctgctcgct gctggtccaa ccggcaaggc
    12721 tcgacatggg aacgccggcg gcgatggaga ccaggagctg atccgtgctc agcgccgtca
    12781 gccccccggc taccgaggcc atgacctgcg gcttgaccgc gaggatcacc acgtccgcac
    12841 cgcgtacctg ggcattgttg tcgtcggagg ttgccacgcc gagctcggcg atcttgccca
    12901 gctgctcggg tgcggggtcg ctggcgcgaa tggtcgccgc atcgtgaccg ctggcgatca
    12961 ggcctttgat caggctggtc gccatgttgc cggcgccaat gaaagtaatg tgactgctca
    13021 acggatcgga actccgggta gtggctcgtt cgggttagcc ctcgcgcgcg ccgaagatgg
    13081 cggtgccgat gcgcacgtgg gtggcgccgg ccattatggc ctgctcgtag tcgttgctca
    13141 tccccatgga cagggtatcc cagcgaccgg cggcgagggg cgcgagcccg gcgaacagct
    13201 tcgccatgcg cgcaaagctc tccggggcat ccgcgttcgg gtcgaggatg gtcatgaggc
    13261 cgcgcacgca gatccgctcg agatcaacga gctccttcag cagatcggcg accgcggctg
    13321 ggtcgacgcc ggccttgccg ggatcggcgt ctatgttgac ctgcagacag acctgcaggg
    13381 ttttgtcttc aggacactgc tcgttgagtc ggcgggcgat cttgggtcga tccaccgtat
    13441 gcacccagtc gaaatgccgc gccacctggc gggtcttgtt cgactgcagc gcgccgatga
    13501 agtgccaggt caggggcaga tgcccgagcg cggcgatctt cggcagcgct tcctgcagat
    13561 agttttcgcc gaagtccttc aagccggcgc cgtgggcctc ttcgatggca cgggccggtt
    13621 gccgcttgga gactgcgatc aggcagacct cgtcggggtc ccgctgtgcg gctcgcgccg
    13681 cagagacaac acaggaacgc acgctggcga cgttgtgaga gatcgagtgc aatttcgttt
    13741 tgccaactgc gtcgcagatt tcgcatgtct ccggcagagc gaggagggat tcatggttaa
    13801 agcccaggtc agataatact atacagcccg gattcgcagc ggctttcgtt gccatttcgg
    13861 cgagtttgaa gcccaagcga gtagacaagg atcgctgatg gacattaccg aactcctcgc
    13921 tttcagcgcc aagcagggcg catcggacct ccacctgtcc gcggggcttc cgccgatgat
    13981 tcgtgttgac ggtgacgttc gccggatcaa cctgccgccg ctcgagcacc agcaggtgca
    14041 ttcgctgatc tacgagatca tgaacgacaa gcagcgcaag gacttcgaag agttcctcga
    14101 aaccgacttc tccgtggaag ttcccggcgt tgcgagattc cgtgtcaacg cgttcaacca
    14161 gaatcgtggc gcgggcgcgg tgttccggac catcccgtcc aaggtgctca ccatggaaga
    14221 cctggacatg gggcaggttt tccgcgacat ctgcctcgtt cccaggggcc tggtgctcgt
    14281 cacgggacct acgggatccg gtaaaagcac gtcgctcgcc gccatggtcg acttcatcaa
    14341 cgaccatcgc tacgaccaca tcctcaccat cgaagatccc atcgaattcg ttcacgagag
    14401 caagaagtgc ctggtgaacc agcgcgaagt gcatcgagat actcacgggt tcaacgaagc
    14461 cctgcgctcg gcgctgcggg aggacccgga catcatcctc gtcggcgaga tgcgcgactt
    14521 ggagacgatc cggctagcgc tcaccgccgc cgaaacgggc cacctcgtgt tcggcacgct
    14581 gcacacgacg tcggcggcca agacca
//
LOCUS       DBNHKV010000131         7222 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_940807, whole genome shotgun sequence.
ACCESSION   DBNHKV010000131 DBNHKV010000000
VERSION     DBNHKV010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7222)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_940807"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="AC16PP_04210"
     CDS             complement(<1..342)
                     /locus_tag="AC16PP_04210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6480860.1"
                     /translation="MTVSAGLGIANFPFQDGPDFWRWVDLCDAGGVDSIWQSDRIISR
                     DPQLECMSVMAALAGGSRRLKFGMNVASLALRDPVLTAKACATIDVLSDGRLLPAFGV
                     GSALSRDFTATG"
     gene            437..1510
                     /locus_tag="AC16PP_04215"
     CDS             437..1510
                     /locus_tag="AC16PP_04215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6480861.1"
                     /translation="MEDRRYLVVGARGLVGRAVLSALEAEDRAEVIGLSRRQPDFPTR
                     ARWIPCDLTDRDACHAALGAAQTQGVTHIVYAALQEMPDLISGWRDPVQIETNARMLA
                     NLLEFVQPREHFTLLQGTKAYGAHLAPMALPGKESAPRHPGENFYWEQEDLMRSRQAG
                     SSWAFSIVRPQIVCGVALGSPMNMVAAIGVYAAVMRQLGKPLCFPGGGGFVTEATDAD
                     LLARAIIWAGREPACANEILNVTNGDVLDWVSLWPAFGEIFQMPTGEADPMRLAISMP
                     ELSGVWDEVVGRHGLKSMAMAELVGDSWQFADAVFGYGGGARSTLLSTIKIRQLGFHE
                     CIDTEQMFRNQLAALQDLKVLPR"
     gene            1510..2457
                     /gene="glk"
                     /locus_tag="AC16PP_04220"
     CDS             1510..2457
                     /gene="glk"
                     /locus_tag="AC16PP_04220"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HIR6480862.1"
                     /translation="MADGAYLVADIGGTNARLALTDGTRLTSPVVISQTTSLRNVAGL
                     AEAARSAFDGQALDGCCIAMAGPVHANQGSLTNVSLAVSGDALGAELGCPARVVNDFY
                     AVARGIPVLRHLRQIGPGEPDEGVRALLGPGSGLGMAVLAPTREGPPLVLPSEGGHAD
                     LAPGSPLEQEVLQVLAARLPHVSWESVLSGPGLVNLYCALCAIWGVPEEALAPEEISR
                     QGLVVEDPICHQVLEMFFGFLGAAAGNLALTVCAEGGVFIGGGIVPKLVEFAESSPLR
                     RRFEERGVLADYAARLPLYVIEDEFPGLLGAVACLADQP"
     gene            complement(2448..3683)
                     /locus_tag="AC16PP_04225"
     CDS             complement(2448..3683)
                     /locus_tag="AC16PP_04225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6480863.1"
                     /translation="MKTKIFDRFLTNGTLAIVDADGSSRQFGCGAPFATMRLNRRGTL
                     TRILRNPELNLGETYLAGEWDVIEGSLHDLLTILRTNLERHAGRSRLARALALMLVPW
                     NHVAASHRNVRHHYDLDETLFRACLDTNMHYSCAYYREANMSLEEAQQAKCDHIAAKL
                     CLRPGMRVLDVGCGWGSLAMELAERHDVTVMGVTLSPEQLRVARSTARLRGLDNHVRF
                     ELQDYRQVEESFDRIVSVGMFEHVGRRHYDEFFGKVEALLADDGVALLHTIGASSPPS
                     VVNPWIERHIFPGGYIPSLSDVAPAVERSGLITADVEVLRHHYALTLQEWNRRFQQRR
                     AEFAQSKGEAFCRMWEFYLLACQTAFEVSDLVVHHWQLAKRNLSVPTTRNYLYETQPY
                     VKIPGETAAEEITLRQHQG"
     gene            3840..4574
                     /locus_tag="AC16PP_04230"
     CDS             3840..4574
                     /locus_tag="AC16PP_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HIR6480864.1"
                     /translation="MAHEIDYRICGESMQYLEVELDPGESVVAEAGTMMYLSEGIAYE
                     ARLGDGSASGVVGKLFGAAKRLLGSESLFMTHFTNEADGPRQMGFAAPYPGQVVAVDL
                     AQAGGEIVCQKDAFLCAAMGTSVSVAFSKRLGAGFFGGEGFILQRLAGDGTAFIHAGG
                     TVLEKRLEGEKLRVDTGCIVGFEPGIDYDIQLAGGLKSMMFGGEGLFLATLEGHGRVW
                     LQSLPFARLADRVLENAVHLERKGES"
     gene            complement(4603..5604)
                     /locus_tag="AC16PP_04235"
     CDS             complement(4603..5604)
                     /locus_tag="AC16PP_04235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6480865.1"
                     /translation="MKAITSITGIDLNEVGPLTRDLEARGFSGVSTQENRFNPFLPLA
                     VAATQSSTLELRTSVAIAFARSPMVSANLGWDLQAASRGRFTLGLGSQVKGHNVRRFS
                     VPWSPPAPRLREYVRALRAIWDCWQNGTPLDFAGEHYQFNLMTPNFTPPPLATAPPRV
                     QLAGVGPATMKVAAEECDGVMLHGFCTRRYVEEAVMPRLETGLAAAGRAREDFEISGG
                     GFVATGKDDAAVAEMFEWVRTRVGFYGSTPSYWPVFEVHGLEDLGHKLNDMSKLGQWD
                     AMAGEISDDVVRLFAAVGRFDEIAGAIEERFGGAVDVVALPASAPPDLLEDIARIAC"
     gene            complement(5609..6733)
                     /locus_tag="AC16PP_04240"
     CDS             complement(5609..6733)
                     /locus_tag="AC16PP_04240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6480866.1"
                     /translation="MASIEFSDEQSLLLETAASFCRDRSPIERVRAGLGDENGCDAAV
                     WQEMVDLGWLGIAAPLEHGGSGLGLAGVVPVVECMGRHLMGSPYVATIQAIEALLGSA
                     SDEQLAQWLPTILAGAPATVALMEPDGSWDVRAATASGESTGNAIRLRGTKTFVLDAA
                     AAELIVTSIDLDGETRLVALRRDQLPDGALNRETVIDETRRSYSLVLDGIRVPRSQIL
                     PGSDFERIERAALLLVCAEIAGGLCGVLHTIVGYLTTRRQFDRLIGSYQALKHPTAEI
                     LLAAERSKSHLYHAATTIGRGGREAEVALRMAKAQGSEAFAWAGDRAVQFHGGFGFTY
                     ECDAQLFLRRALWCQYQLGDERYHRRKLAPLLLAATDTEH"
     gene            complement(6742..>7222)
                     /locus_tag="AC16PP_04245"
     CDS             complement(6742..>7222)
                     /locus_tag="AC16PP_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6480867.1"
                     /translation="IAMITLTFERGATGGQAGGLSAMDLNINDVVELARRARRDGGPA
                     LADPLIQDELVQLMIESRGNALMADRTTIPALASDWPEAIAMSGKLRATELKRRMCRF
                     ALAMQGGNGARYVSADAVDGGMWQRSYLNAFSGTIGGGTSEIQRNILGERVLGLPKD"
BASE COUNT         1161 a         2336 c         2488 g         1237 t
ORIGIN      
        1 gcccgtggcg gtgaaatctc tggacagagc gctgcccacg ccgaacgcgg ggaggagccg
       61 cccgtcgctg agcacgtcga tggtggcgca ggccttggcg gtgagcacgg ggtcgcgaag
      121 cgccaggctc gctacgttca tgccgaactt caggcgccgg ctgccgccgg ccagcgcggc
      181 catgacgctc atgcattcca gctgcgggtc gcgactgatg atccgatcgc tctgccagat
      241 tgaatccacc cccccggcgt cgcacagatc tacccagcgc cagaagtctg gcccgtcctg
      301 gaacggaaaa ttcgcgatgc cgagtccggc gctgaccgtc atgaatcaat cctggcttcg
      361 ggggaaccgg attatagcgg gccgagttgg tggcctggca attgaaccgg atgggtggca
      421 tgcggacaat ggcggcatgg aggatcgcag atatctggtc gtgggggccc gggggctcgt
      481 cggccgcgcc gtgctgtcgg ctctggaggc tgaagatcga gcagaggtca tcgggctttc
      541 ccgtcggcag ccggactttc ccacccgggc gcgctggatc ccctgcgatc tgacggaccg
      601 cgatgcgtgc cacgcggcgt tgggcgccgc gcagacgcaa ggcgttaccc acatcgtcta
      661 cgccgctctt caggaaatgc cggatctgat atcgggctgg cgggaccccg tccagatcga
      721 aaccaacgcg cggatgctcg ccaacctgct ggagttcgtg cagccacggg agcacttcac
      781 gctgctgcag gggaccaaag cctacggcgc tcatctcgcg cccatggcgt tgccggggaa
      841 ggagtcggcg ccccggcacc cgggcgagaa cttctactgg gagcaggaag acctgatgcg
      901 cagccgccag gccggatcgt cctgggcctt cagcatcgtc cgcccacaga tcgtctgcgg
      961 cgtagcgctc ggcagcccca tgaacatggt ggcggccatc ggtgtttacg ccgccgtgat
     1021 gcgccagctc ggcaagccgc tttgctttcc gggtggcggc ggtttcgtta ccgaggccac
     1081 ggacgccgac ctgctggccc gcgcgatcat ctgggccggg cgcgaacccg cctgcgccaa
     1141 cgagatcctc aacgtcacca acggcgacgt gctcgattgg gtctccctgt ggcccgcgtt
     1201 cggtgagatt tttcaaatgc ccaccggcga agcggacccc atgcggctcg cgatcagcat
     1261 gccggaactc tccggtgtct gggacgaggt ggtcggacgg cacgggctta agtccatggc
     1321 catggccgag ctggtgggcg attcatggca gttcgccgac gcggtattcg gctatggcgg
     1381 cggggcgcgg agcacgctgc tgagcacaat caagattcgc cagctcggct tccacgaatg
     1441 catagacacc gagcagatgt tccgcaacca gcttgccgcc ctgcaggatc tcaaggtgct
     1501 gcctcgctga tggcggacgg agcgtatctg gtcgcggaca tcggtggcac caatgcccgc
     1561 ctggcgttaa ccgacggaac gcggctcacg tcccccgtgg tgatttcgca gacgacctcg
     1621 ctcaggaacg tggccggtct cgccgaagcc gcgcgttcgg ctttcgacgg ccaggcgttg
     1681 gacgggtgct gcatcgccat ggcgggcccc gttcacgcca atcagggcag cctgacgaac
     1741 gtgtcgctgg ccgtttcggg ggacgcgctg ggtgccgagc tcggctgtcc cgcccgggtg
     1801 gtcaacgatt tctacgccgt ggctcggggc atcccggtcc tgcgccacct gcgtcagatc
     1861 ggaccgggcg agcccgacga gggcgtcagg gcgctgcttg gacccggcag cgggttgggg
     1921 atggccgtgc tggcgccgac ccgggaaggt ccgcccctgg tgcttccgtc cgagggcggg
     1981 cacgccgacc tcgcgcccgg ctctcccctg gagcaggagg tgctgcaggt gcttgccgca
     2041 aggctccccc acgtcagctg ggagagtgtg ctgtcaggac ccggcctggt gaacctctac
     2101 tgcgcgctat gcgccatctg gggagtgccg gaggaagcgc tggctcccga ggagatcagc
     2161 aggcaggggc tggtcgtgga ggacccgatc tgccatcagg tgctggaaat gttcttcggc
     2221 tttctcggcg ccgcggcggg gaacctcgcg ctcaccgtct gtgcggaagg cggcgtattc
     2281 atcggcggcg gcatcgtgcc caagctcgtc gagttcgcgg agtccagccc cctgcgcagg
     2341 cgcttcgagg aacgcggcgt gttggccgac tacgcagcgc ggctgccgct ctacgtcatc
     2401 gaggatgagt ttccgggtct gcttggcgcc gttgcctgcc tggccgatca gccctgatgc
     2461 tgcctgaggg tgatctcttc ggccgcggtt tccccgggga tcttcacgta cggctgcgtc
     2521 tcgtacagat agttgcgcgt ggtgggcacg ctcaagttgc gcttggccag ctgccagtgg
     2581 tgaaccacca gatccgacac ctcgaaggcg gtctggcacg cgagaaggta aaactcccac
     2641 atgcggcaga acgcctcgcc tttgctctga gcgaactcgg cgcgcctttg ctggaaccgg
     2701 cggttccact cctggagcgt cagcgcgtag tgatgacgaa ggacctccac gtcggcggtg
     2761 atcagcccgc tgcgctccac ggctggcgcc acgtcggaga gtgaagggat gtagccgccg
     2821 ggaaaaatgt ggcgctcgat ccacgggttc accacgctgg gcgggctgga cgccccgatg
     2881 gtgtgcagca gcgcaacgcc gtcgtctgcg agcagcgctt cgaccttgcc gaagaactcg
     2941 tcgtagtggc gacggccgac gtgctcgaac atgccgacgg agacgatcct gtcaaagctc
     3001 tcctctacct gccggtaatc ctgaagctcg aagcgcacgt gattgtcgag gccccgcagg
     3061 cgggccgtgc tccgggccac acgcagctgc tccggcgaga gggtaacccc catcaccgtt
     3121 acgtcgtgcc gctcggcgag ttccatcgcc aggctgcccc agccgcagcc aacgtcgaga
     3181 acgcgcatgc cggggcgcag gcagagcttg gccgcgatgt ggtcgcactt ggcctgctgt
     3241 gcctcctcca ggctcatgtt cgcttcgcgg tagtacgcgc acgagtagtg catgttggtg
     3301 tccaggcacg cgcgaaagag cgtctcgtcc agatcgtagt gatggcggac gttgcggtgg
     3361 ctggcggcca cgtggttcca ggggacgagc atgagcgcga gcgcccgggc aagccgcgat
     3421 cgaccggcgt gacgctcgag attcgtgcgc aaaatggtga gcagatcgtg cagagagccc
     3481 tcgatgacgt cccactcgcc tgccaggtag gtctcgccca ggttgagctc gggattgcgc
     3541 agaatccggg tcaacgtccc gcgccggttc aagcgcatgg tggcgaacgg agcgccgcag
     3601 ccgaactgac ggcttgaacc atcggcgtcg acgatggcca gcgtcccgtt ggtgagaaac
     3661 cgatcgaaga tctttgtttt cacgacgtca tgccccctgc tcggcggccg gttctttgcc
     3721 agccatcccc tcacatggca ataattatcg cgctgcgggt gttttttcaa ctagaccttt
     3781 tggcgtcgcc gggggccaga aggtgcaggt cggggcttga gaacgaggag gaccgcgcga
     3841 tggcgcacga gatcgactac cggatttgcg gtgaatccat gcagtacctc gaagtagaac
     3901 tcgatcccgg ggagtctgtg gtcgccgaag cgggcaccat gatgtatctg agcgagggca
     3961 ttgcctacga ggcacggctc ggggacgggt cggccagcgg ggtggtgggt aaactcttcg
     4021 gtgcggccaa acggctgctc ggcagcgagt cgctgttcat gacgcacttc accaacgaag
     4081 ccgacggccc gcggcagatg ggctttgcgg ctccgtatcc cgggcaggtg gtcgcggtgg
     4141 atctggcaca ggcaggtggc gagatcgtct gtcagaagga cgcttttctc tgcgcggcga
     4201 tgggcacctc cgtgtccgtg gcgttcagca agcgtcttgg tgcgggcttc ttcggtggcg
     4261 agggctttat ccttcagcgt ctggccggtg acggcacggc gttcattcac gccgggggaa
     4321 ccgtgctaga aaaacgtctc gagggcgaga agcttcgcgt ggacaccggc tgcatcgtcg
     4381 gcttcgagcc gggcatcgac tacgacatcc agcttgccgg cggcctgaaa tccatgatgt
     4441 tcggtggcga gggcctgttt cttgccactc tggaggggca tggacgggta tggctgcaat
     4501 ccctgccgtt cgcgcggctg gccgacaggg tcctggagaa cgccgtgcac ctggaacgca
     4561 aaggcgagtc ctgaaccgga tccggcggct cctgaggggg actcagcaag cgatccgggc
     4621 gatgtcctcg agcaggtcgg gcggggctga ggcgggcagg gcaaccacgt ccacggcgcc
     4681 gccaaagcgc tcctcgatgg caccagcgat ctcgtcgaaa cggcccaccg cggcgaagag
     4741 ccggaccacg tcgtcagata tttctccggc catggcatcc cactggccga gcttggacat
     4801 gtcgttcagc ttgtggccga gatcctcgag cccgtgcacc tcgaatacgg gccagtagga
     4861 gggggtggaa ccgtaaaagc ccacgcgggt tcggacccac tcgaacatct ccgcaacggc
     4921 agcatcgtct ttgcccgtgg cgacgaaccc accgccggag atctcgaagt cctcgcgcgc
     4981 gcgcccggcg gcggccaggc ccgtctcgag gcgcggcatc accgcttcct ccacgtatcg
     5041 gcgcgtgcag aacccatgca gcatgacccc gtcacactcc tcggctgcaa ccttcatcgt
     5101 ggcgggcccg acgccggcca gctgcacccg gggcggggcg gtggccagcg gcggtggggt
     5161 gaagttcggc gtcatcaggt tgaactggta gtgctcgccg gcgaaatcga gcggggtccc
     5221 gttctgccag cagtcccaga tggcccgaag agcgcggacg tactcgcgca gccttggcgc
     5281 cggcgggctc cagggaacgc tgaagcggcg aacgttgtgc cctttgacct gactgccgag
     5341 gccgagcgtg aatcggccgc gggaagccgc ctgcaggtcc cagccgagat tggcggaaac
     5401 catggggctg cgcgcgaagg caatcgccac gctggttctc agctccaggg tcgacgactg
     5461 ggtggccgcc acggccagcg ggagaaaggg gttgaaacgg ttttcctggg tggacacgcc
     5521 gctgaacccg cgcgcttcga ggtcccgggt caggggcccg acctcgttga ggtcgatgcc
     5581 ggtgatggag gtaatggcct tcatgagctc aatgctccgt gtctgtcgct gctaggagta
     5641 gcggcgcgag cttgcgccga tgatagcgtt cgtcaccaag ctgatactgg caccatagcg
     5701 cccggcgcag aaacagctgg gcgtcgcact cgtaggtgaa gccgaagccc ccgtgaaact
     5761 gcaccgcccg gtcaccggcc cacgcgaagg cctcgcttcc ctgtgctttc gccatccgca
     5821 gggcgacctc ggcttcgcgc ccgccccggc cgatcgtggt ggctgcgtgg tagaggtgtg
     5881 acttgctgcg ctcggccgcg agcaggatct cggccgtggg gtgcttgagc gcctggtagc
     5941 tgccaatgag gcggtcgaac tggcgccgcg tggtcaggta gccgacgatg gtgtgcagca
     6001 cgccgcaaag cccgccggcg atctccgcgc agacgaggag cagggccgcc cgctcgatcc
     6061 gttcgaaatc gctgcccggc agaatctggg atcgcggtac cctgatgccg tccaggacca
     6121 ggctatagct gcggcgggtc tcgtcgatca ccgtctcgcg gttgagcgcc ccatcgggca
     6181 gctgatcgcg gcgaagggcc accagccggg tttcgccgtc gaggtcgatg gacgtcacga
     6241 tcagctcggc ggccgccgcg tccagcacga aggtcttggt gccgcgcagg cgaatggcgt
     6301 ttcccgtcga ctctccgctg gcagtggccg cgcggacgtc ccagctgccg tcaggctcca
     6361 tcagcgccac ggttgccggc gccccggcca gtatcgtcgg cagccactgc gccagctgct
     6421 cgtcgctggc cgagcccagc agcgcttcaa tggcctggat ggtggcgacg tagggtgacc
     6481 ccatcaggtg gcggcccatg cactcaacca ccggaacgac gcctgccaga ccgagccctg
     6541 aaccgccgtg ctccagcggg gcagcgatcc ccagccagcc gaggtctacc atctcctgcc
     6601 acacggcggc gtcgcagccg ttttcgtcgc cgaggccggc gcgcacccgc tctatcgggg
     6661 atcgatcgcg acagaacgag gcggcggttt ccaggagcag cgactgctcg tctgagaatt
     6721 cgatggacgc cacggtggtg ctcaatcctt cggcaggccg agtacccgct ctccgaggat
     6781 gttgcgctga atttcagacg tgcccccgcc gatggtgccc gagaacgcgt tcaggtagga
     6841 gcgctgccac atgccgccat ccacggcatc cgcgctcacg tagcgggcgc cgttgccgcc
     6901 ctgcatggcc agcgcgaatc ggcacatgcg gcgtttgagc tcggtggcgc gcagcttgcc
     6961 cgacatggca atggcttcgg gccagtcgga ggcgagcgcg gggatggttg tgcggtctgc
     7021 catcagggca ttcccccggg actcgatcat cagctgcacc agctcgtcct gaatcagcgg
     7081 atccgccagc gccggaccgc cgtcgcgacg agcgcgcctg gccagctcca ccacgtcgtt
     7141 gatgttgaga tccatggcgg acagcccgcc ggcctggccg cccgtcgcgc cgcgctcgaa
     7201 cgtgagcgtg atcatggcga tg
//
LOCUS       DBNHKV010000132         5731 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_303007, whole genome shotgun sequence.
ACCESSION   DBNHKV010000132 DBNHKV010000000
VERSION     DBNHKV010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_303007"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="AC16PP_04250"
     CDS             complement(<1..1190)
                     /locus_tag="AC16PP_04250"
                     /inference="COORDINATES: protein motif:HMM:NF018444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6480868.1"
                     /translation="MSEALLNDELKGVIQTGYRAWLDARGFRPRRGQREMIALIARTL
                     TGDAPRIVAVEAGTGTGKTAAYCLAAIPTGAALDKTVVISTATVALQEQVVLRDLPDL
                     ASRSGLTFSFTLAKGRGRYVCLKRLDDRLRFAEPSEIPLFEDTGEEGAALYQAMLNAF
                     ANKTWNGDRDEWTEEVDPQLWRAVTTDHRGCSNNRCGFFRQCPFFKARGALDAADVIV
                     ANHDLVLADLSLGGGVVLPPPEDCIYVLDEAHHLPEKTQQHFSASTRVLATGQWVDNV
                     ARVIGSLTQRFGRPDELVSLASRASEHGAAFAEAMMRMGDDLQGLSFEPRGEGLATHR
                     FRLGQVPAELAEQAQLALEPLTLLAGVISAVHELLQKAVAGELPWDNGFEAEDWLPAV
                     GQLET"
     gene            complement(1236..1853)
                     /locus_tag="AC16PP_04255"
     CDS             complement(1236..1853)
                     /locus_tag="AC16PP_04255"
                     /inference="COORDINATES: protein motif:HMM:NF039960.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR6480869.1"
                     /translation="MGRARVARAAVLGLSVVVMAGCASAPPSKPDQICSIFREKSGWY
                     KDARRAEKRWDMPISVGMAFVYKESSYRSDARPERERLLWVVPWRRPSSAFGYAQITD
                     AAWREYLDDTRGFLRERDDFGTALDFVGWYNDRSHRRLGIAKNDAYNLYLAYYVGHGG
                     YKRGHHRKKPEVRRYARRVSEKSKRYAAQLSRCEKEFRKPWWRPI"
     gene            complement(1846..3888)
                     /gene="ligA"
                     /locus_tag="AC16PP_04260"
     CDS             complement(1846..3888)
                     /gene="ligA"
                     /locus_tag="AC16PP_04260"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR6480870.1"
                     /translation="MTITRAGAGSRISELRRLVRHHNHRYYVLDSPEIADAEYDALYD
                     ELVALEGDHPDLVTDDSPTQRVGGAPLEQFNSVTHEVAMLSLDKCTTHEELADWIERC
                     RGRLEEGEALDFVCEPKVDGVAVALTYENGRLVLGATRGDGQTGEDITANVRTVGAIP
                     LVMRDDAGHDFPPRIEVRGEIYMPVADFEAFNEQARQRDEKTLVNPRNGAAGSLRQLD
                     PKVSASRPLTMYCYSIGWVEGDWQPRTHMEALERLADWGFRVNPDARAVSGLAGCLRY
                     VEEMLERRDSLGYDIDGVVIKVNDLDQQRRLGAVTRKPRWSIAFKYPAEEATTTLIGV
                     EFQVGRTGAITPVARLAPVFVGGVTVSNATLHNMDEVRRLDIRVGDTVMVRRAGDVIP
                     QVVSVIDSRRPPGAEAVALPQGCPSCGSPITRPDDEAVARCSAGPEICPAQRKEGLRH
                     FASRLALDIEGLGDKLIEQLVDKDQVRIAADLFSLRKDELIRLERMGAKSAENLLAAI
                     ERSRETTLARFIYALGIREVGEATAQSLARHFGDLEPLRRAQVAELEQVPDVGPVVAG
                     NIHSFFANDDGARAVDALLEAGIRWPAVAVETGAQPLLGQTWVLTGTLESMTRDAAKS
                     ELVALGAKVSGSVSGKTSQVVAGPGAGSKLEKARDLGVPVMDEAEFLEVLESHRHG"
     gene            complement(3885..4838)
                     /gene="zipA"
                     /locus_tag="AC16PP_04265"
     CDS             complement(3885..4838)
                     /gene="zipA"
                     /locus_tag="AC16PP_04265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HIR6480871.1"
                     /translation="MDIKDFILIGGGLLIAGVIAHGFYIAWRGRRDTLRMDIAHDLIP
                     EAVDDLHRLRGELPNGGARVVSQASLGEQASLELDTVVVAEPLDAQPPEPAMLAEESA
                     VGESVKAREPTIGADDAPRTPAGEKDQKRPGAPRVADVTVAPEPIVAERPRRTRRPPA
                     RKAQETAPEPALEQPPVEELVVMNVLAPRGQPFAGDELFASLRGRGLKFGNMNIFHRL
                     DAGSKKVQFSVANIVEPGTFEMSDMETIRSPGLCFFLQLPGPENPLEAFETMLSVAHG
                     VAEELNGELKDEQRSFMTPQTEEHYRQRILEFSRRRMSKRA"
     gene            complement(4904..>5731)
                     /locus_tag="AC16PP_04270"
     CDS             complement(4904..>5731)
                     /locus_tag="AC16PP_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation SMC family protein"
                     /protein_id="HIR6480872.1"
                     /translation="RGEAEAAVETVRSKLETTRVERQGLAVQQSNLTDQIAATGLGAD
                     EVREGLAEDAEEARWVELLERTDRRIQRLGPINLAAIEEFEAQSERKVYLDKQNEDLE
                     QALETLLGAIRRIDRETRARFKETFETVNRHLGELFPKVFGGGHAYLELTGEDLLDTG
                     VSLMARPPGKRNSSVHLLSGGEKAMTAVALIFAIFHLNPSPVCMLDEVDAPLDDVNVM
                     RFAELIKDMSTDVQFVVITHNKLTMEMADHLMGVTMNEPGVSRLVSVDVEEASAMAG"
BASE COUNT          923 a         2001 c         1800 g         1007 t
ORIGIN      
        1 gtttccagct gccccacggc cggcagccag tcctccgcct cgaagccgtt gtcccagggc
       61 aactcgccgg caaccgcttt ctgcagcagc tcgtggacgg cggagatcac gccggcaagg
      121 agcgtgagcg gctccagcgc cagctgcgcc tgctctgcca gctccgctgg tacctgtccc
      181 agcctgaagc ggtgcgttgc caggccttca ccgcgcggtt cgaagctgag cccctggaga
      241 tcgtccccca tgcgcatcat ggcttcagcg aacgcggcgc cgtgctccga ggccctgctg
      301 gcgagactga cgagctcgtc cggtcgcccg aaacgctgcg tcaagctgcc gatgacccgg
      361 gcaacgttgt ccacccactg cccggtggcg aggacccgcg tggaggccga gaaatgctgc
      421 tgggttttct ccggcagatg gtgcgcctcg tcgagaacat agatgcagtc ctccggcggc
      481 ggcagaacga cgccgccacc gaggctgagg tcggccagca ccaggtcgtg gttggcgacg
      541 atgacgtcgg cggcgtcgag cgctcccctg gccttgaaga acgggcactg ccgaaagaag
      601 ccacaccgat tgttgctgca tccccgatgg tccgtggtca cggcccgcca cagctgcggg
      661 tcgacctcct cggtccactc gtcgcgatcg ccgttccagg ttttgttcgc aaacgcgttg
      721 agcatggcct ggtaaagggc cgcgccctcc tcgcccgtgt cctcgaacag ggggatctca
      781 gagggctccg caaatcgcag ccgatcgtcg agccgcttca ggcagacgta gcgcccccgc
      841 cctttggcca gggtgaaact gaacgtcagg ccagaacggc tcgccagatc ggggaggtcg
      901 cgcaggacca cctgctcctg cagggccacc gtcgcggtgc tgataaccac cgtcttgtcc
      961 agagcagcgc ccgtggggat ggccgccagg caataggccg ccgtcttgcc cgtcccagtg
     1021 cccgcctcca cggccacgat gcgcggcgcg tcaccggtca gcgtccgggc gatcagcgcg
     1081 atcatctccc gctggccgcg tcggggccga aagcccctcg cgtcgagcca ggcacggtat
     1141 ccggtctgaa taacgccttt cagctcgtcg ttgaggaggg cctcgctcat cgagcgagct
     1201 tacgttgccg gtcgatcact ggccaccggc aacgctcaaa tcggccgcca ccagggcttg
     1261 cggaactctt tctcgcagcg cgacagctgc gccgcgtatc gcttgctctt ctccgagacc
     1321 cgtcgagcat aacggcggac ctccggcttc ttccggtgat gtccccgctt gtagccgcca
     1381 tggccgacgt agtaggcgag atagagattg taggcatcgt ttttggcgat gccgagacgc
     1441 cggtgactgc ggtcgttgta ccagccgacg aaatccaggg ccgtgccgaa gtcgtcgcgc
     1501 tcgcgcagga accctctggt gtcgtcgagg tattcccgcc aggcggcatc ggtgatctgt
     1561 gcgtaaccaa acgccgagga cggccgccgc cagggcacta cccacaacag gcgctcacgc
     1621 tccggcctcg cgtcggaccg gtaggacgac tccttgtaga cgaacgccat gcccacggag
     1681 atgggcatgt cccagcgctt ctcggcacgc ctggcgtctt tgtaccaacc ggatttttcc
     1741 cggaagatcg agcagatctg atccggcttc gacggcggcg cgctggcgca cccggccatc
     1801 acgaccacgc tcaagccgag aacggcggcc cgagcgacgc gagccctacc catggcggtg
     1861 gctctccagg acctcgagaa actcggcttc gtccatcacc ggcaccccca ggtcgcgcgc
     1921 cttttccagc ttgctgccgg cgcccgggcc ggccaccacc tgggacgtct tccccgacac
     1981 cgagccgctc accttggcgc ccagggcaac gagctccgac ttggccgcat ccctggtcat
     2041 gctctccagc gtgccggtca gcacccaggt ctgcccgagg agcggctgcg cgccggtctc
     2101 gacggctacc gccggccacc ggattcccgc ctccagcagc gcgtctacgg cccgggcgcc
     2161 gtcgtcgttg gcaaagaagc tatgaatgtt gccggcgacc accgggccca cgtccggcac
     2221 ttgttcgagc tcggctacct gcgcccgccg aaggggttcc agatcgccga aatgacgggc
     2281 caggctctgg gcagtcgctt cccccacctc gcgaatgccc agagcgtaga tgaaacgcgc
     2341 aagcgtggtc tcgcggctgc gctcgatggc ggccagcagg ttctcggccg acttggcgcc
     2401 catgcgctcc agacggatca gctcgtcctt gcgcaggctg aagagatccg cagcgatcct
     2461 cacctgatcc ttgtccacca gctgctcgat gagcttgtcg ccgaggccct cgatgtccag
     2521 tgccagacgg ctggcaaagt ggcgcaggcc ctctttgcgc tgggcgggac agatctcggg
     2581 gccggccgag cagcgcgcaa ctgcctcgtc gtccgggcgc gtgatgggac tgccacacga
     2641 cgggcacccc tgaggcagcg ccaccgcttc ggcgccgggc gggcgtctgg aatcgatgac
     2701 gctgacgacc tggggaatga cgtcgcccgc ccgcctcacc atgacggtgt cgcccacgcg
     2761 gatgtccagg cgccggacct cgtccatgtt gtggagcgtc gcgttgctca ccgtcacgcc
     2821 gcccacgaag acgggcgcca gacgagccac cggggtaatg gctccggtac gacccacctg
     2881 aaactccacg ccaatcaggg tcgtggtcgc ttcttccgcc gggtacttga aggcgatgga
     2941 ccatcgcggc ttgcgggtga cggcccccag gcgccgctgc tgatcgaggt cgttcacctt
     3001 gatcacgaca ccatcgatgt cgtagcccag cgagtcgcgg cgctccagca tttcctccac
     3061 gtaccgcagg catccggcga gcccgctcac cgcacgggcg tcgggattga cacgaaaacc
     3121 ccagtcggcg agacgctcca gggcttccat gtgcgtccgg ggctgccagt ctccttccac
     3181 ccagccgatg ctgtagcagt acatggtcaa cgggcggctt gcgctcacct tcggatccag
     3241 ctggcgaagg ctgcccgcgg ctccgttgcg cgggttgacc agcgtcttct cgtcgcgctg
     3301 acgcgcttgc tcgttgaacg cctcgaaatc ggcgacgggc atgtagatct cgccgcgcac
     3361 ctcgatgcgc ggcggaaagt cgtgccccgc atcgtcccgc attaccaggg gaatcgcgcc
     3421 gacggtgcga acgttggcgg tgatgtcctc tcccgtctgc ccgtcccccc gggtggctcc
     3481 gagaaccagc cgaccgttct cgtaggtcag ggcgaccgca acgccatcca ccttgggctc
     3541 gcagacgaag tcgagcgctt ctccctcttc cagacgtccg cgacaacgct cgatccagtc
     3601 cgcgagctcc tcgtgggtcg tgcacttgtc cagggagagc atggccacct cgtgggtgac
     3661 cgagttgaac tgctcgagcg gcgccccccc gacccgctgg gtgggcgaat cgtcggtcac
     3721 gagatcggga tgatcgccct ccagagcaac gagctcgtcg tacaacgcgt cgtactccgc
     3781 gtcggcgatc tctggagaat cgagcacgta gtaacgatgg ttatggtggc gtaccagccg
     3841 acggagctcg ctgatgcggc tcccggctcc ggccctggtg atggtcatgc ccgcttggac
     3901 atgcgccgcc gcgagaactc caggatccgc tggcggtagt gctcctcggt ctggggcgtc
     3961 atgaagctgc gctgctcgtc tttcagctcg ccgttgagct cttcggcgac gccgtgggca
     4021 acagacagca tggtctcaaa cgcctcgagc ggattttcag gtcccggcag ctgcagaaag
     4081 aagcagagtc ccggcgagcg gatggtttcc atgtccgaca tctcgaacgt ccccggctcg
     4141 acgatgttcg ccacgctgaa ctgaactttc ttggagcccg catccaaacg atgaaagatg
     4201 ttcatgttgc cgaacttcag gccgcgtcct cgcagcgacg cgaagagctc gtcgcccgca
     4261 aacggctgcc cacggggcgc gagcacgttc atcaccacga gttcttccac cggcggctgc
     4321 tcgagcgcag gctcaggagc cgtttcctgg gccttgcggg ccggcgggcg gcgcgtgcgc
     4381 cgcggtcttt ccgcgacgat gggctccggg gccacggtga cgtcagccac ccgcggggcg
     4441 cccgggcgtt tctgatcttt ttctccggca ggcgtccttg gcgcgtcgtc cgctccgatg
     4501 gtgggctctc gtgccttcac cgactccccg accgcgctct cttcggccag catcgcaggc
     4561 tcgggcggct gcgcgtccaa gggctcagcc accaccacgg tgtcgagctc gaggcttgcc
     4621 tgctcaccga gagacgcctg cgacaccacg cgggcgcccc cgttgggcag ctcgccgcgc
     4681 aaccgatgaa gatcgtcgac ggcctcggga atcaggtcgt gagcaatgtc catgcgcagg
     4741 gtatcgcgac gaccgcgcca ggcaatgtag aacccgtggg caatcacccc ggcgatcagc
     4801 aggccgccgc cgatcagaat gaaatctttg atgtccacaa ccggctcctc ggccgcctgg
     4861 gcgacctgac acgtccagag ggtgaagctt ccggcgacat ggctcagcca gccatcgccg
     4921 aggcttcttc tacgtctacc gagacgagcc gcgatacgcc gggctcgttc atggtaacgc
     4981 ccatgagatg gtcggccatc tccatggtga gcttgttgtg ggtgatcaca acaaactgca
     5041 cgtcggtaga catatcctta atcagctcgg caaaacgcat gacgttcacg tcgtcgagcg
     5101 gcgcgtccac ctcgtcgagc atgcagaccg ggctcgggtt gaggtggaag atggcaaaaa
     5161 tcagagccac cgccgtcatc gccttctccc cgcccgaaag caggtgaacg ctggaattgc
     5221 gtttgcccgg cggcctcgcc atgagcgaaa ccccggtgtc gagcagatcc tcccctgtca
     5281 gctccagata ggcgtgtcct ccgccgaata ctttcgggaa cagctctccc agatgtctgt
     5341 tcaccgtctc aaaggtttcc ttgaagcgtg cgcgagtctc gcgatcgatc ctgcgaattg
     5401 cgccgagcag ggtttccagc gcctgctcca ggtcctcgtt ctgtttgtcg aggtagacct
     5461 tccgctccga ctgcgcctcg aactcctcga tggcagccag gttgatgggg ccaaggcgct
     5521 ggatgcgccg atcggtcctt tcgagcagct cgacccagcg ggcctcttcc gcatcctcgg
     5581 ccagcccctc gcgcacctcg tccgcgccga ggccggtggc ggcgatctga tccgtgaggt
     5641 tggactgctg cacggcgagt ccctggcgct cgacccgcgt ggtctcgagc ttcgagcgaa
     5701 cggtctccac cgctgcttcc gcttcgccac g
//
LOCUS       DBNHKV010000133        18543 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1436514, whole genome shotgun sequence.
ACCESSION   DBNHKV010000133 DBNHKV010000000
VERSION     DBNHKV010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 18543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18543
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1436514"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="AC16PP_04275"
     CDS             <1..403
                     /locus_tag="AC16PP_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6480873.1"
                     /translation="NPMADIPYEYYVDIVTKRYFHGVDKCDMDMVLDCFHEDAVLTEV
                     TSMTVHEGRDEGIRRMFEGLFDAHSRIWHGNFVHTADVDGEAICSQFSVEVTPKETGT
                     ELRYENCNRFYLDNGKFSRVFVYMSGENLLK"
     gene            431..829
                     /locus_tag="AC16PP_04280"
     CDS             431..829
                     /locus_tag="AC16PP_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6480874.1"
                     /translation="MQYQSSLSRSGLETLAIESYFGNVDAKNLDAVLDCFHDNAMITV
                     QTAFTTHVGKDGIRRMFVDFMGAYKTIIHRDFTCTIDEKNGRIAACFTAELEDHDGNW
                     TTLQNTNFWRVRGDRFQEVYIYMSGENPLV"
     gene            complement(872..1828)
                     /locus_tag="AC16PP_04285"
     CDS             complement(872..1828)
                     /locus_tag="AC16PP_04285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6480875.1"
                     /translation="MRAVYYERFGGSEVLTVGELPTPEPASGEVLIRIHAASVNPVDW
                     KIREGFFDGIFPHRFPIIPGWDAAGEVAGLGDGVTEMQIGSRVYAYCRKPVVHGGTYA
                     ELVTVPAGFVAPAPANLDDVETSTIPLAGLTVWQSLFEFAGLTAGQTILIHAAAGGVG
                     SLAVQLAAHAGARVFATTSAANAAYVRELGAHRIIDYRQENLGEVMGSEVPAGFDVVF
                     DTVGGEVLGESYDLVRPGGALPALNDAPDEALCAQRGIRGLRVFSEPNGQHLSRLTGL
                     IEAGALRPAQIESFPLEQAALAMDRSQAGHVRGKLALAVGQR"
     gene            1863..2261
                     /locus_tag="AC16PP_04290"
     CDS             1863..2261
                     /locus_tag="AC16PP_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR6480876.1"
                     /translation="MEPPTEPRLIKTDPDPFEPFCIAQGYQVGNLIFLSGQAALDLQG
                     NVVGSGDFDLQAEQALRNVQMLLDQAGSGLDKLIKVTIYLTDMTQFPKIVELRRKWFT
                     PPYPADTIVEVRSLALPELMIEIDAIALAG"
     gene            complement(2273..2974)
                     /locus_tag="AC16PP_04295"
     CDS             complement(2273..2974)
                     /locus_tag="AC16PP_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6480877.1"
                     /translation="MIDLYYWPTPNGHKVAIMLEEIGSEYQLHPVNILEGEQFDPDFL
                     EISPNNRIPAIVDRSGPDGTPYALFESGAILLYLAEKTGVLWPSSTAARYDMLQWLFF
                     QNANVGPMFGQCGHFQGYAPEPVPYAIERYHNETRRLYGVMDRRLASCAYLGGPAYSL
                     ADIATYPWCMPIIRELHKIDIDEFPNVADWADRIAQRPAVARGTSLLAEVMKIGDPDE
                     KTREAFFGSRQLEQR"
     gene            complement(2971..3708)
                     /locus_tag="AC16PP_04300"
     CDS             complement(2971..3708)
                     /locus_tag="AC16PP_04300"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6480878.1"
                     /translation="MWIFYSPFHTFIHKVLVTAHECGHWDDITFVATFPFKNNEGIDQ
                     GDAYDISALNPLNKVPTLVTGEGQVIYGSQAACEYLDGTSRARRMYPEPGPARWDAVT
                     RLALSDTIFEQTVVMVMEQWKPESEWNRGLFEWLWPKFIAGLDKLERDAERGWGDFDV
                     GHAAMLHAISYLGFRAEFYEAKDPIHPDFRWRDGRPALSAWFDEAVERPSVQSHYLKD
                     FVGDSSAEFCQAQVQAVLAAQKQHDTG"
     gene            3856..4254
                     /locus_tag="AC16PP_04305"
     CDS             3856..4254
                     /locus_tag="AC16PP_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480879.1"
                     /translation="MKFVGYLIGSVVILVVLIFVSQLVASESAEVVVLTSQGPDGGQD
                     TRLWVVDRDGLQYLRASPDSGWYQRLAAEPQVLLVRNGNSAAYRAEIRMALAEDVNRL
                     MREKYGWRDAYIEFLIGDRMAAVPVALIPD"
     gene            4423..5442
                     /locus_tag="AC16PP_04310"
     CDS             4423..5442
                     /locus_tag="AC16PP_04310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6480880.1"
                     /translation="MKDLKLGLQLGYWGAGPNPDMVKVAQEAERLGYDAIFTAEAWGS
                     DVFTPLAWIGAHTERIRLGTGIAQLSARAPTATAMAALTLDHLSKGRVILGLGVSGPQ
                     VVEGWYGRPFGKPLARTREYVSIIRQVLERKEPVTNDGEHYPLPYTGEGSWGLGKPLK
                     SITHPYRSDLPIFLGAEGPKNVAMTAEIADGWLPLYYSPYRQEVYADQIAHAKPGFEI
                     MQGVSVIINDDVEEALIPLKRNLALYIGGMGAKSRNFHNELVQRMGFADAAAKIQDLF
                     LSGRKDDAAAAVPTELADEISLVGSVERIKDRLQAWRETPVTSLLVSTQNISQLRTFA
                     EIVLG"
     gene            5551..6009
                     /locus_tag="AC16PP_04315"
     CDS             5551..6009
                     /locus_tag="AC16PP_04315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6480881.1"
                     /translation="MSDVKDEDRAAFFEAVDAACKKAVWCALASVAGDRPRVRIVHPT
                     WDGEVLWFATGPDSPKARQLRENPHVDIQYQVAPPDFVHVMVRGTAELVTDQAAKNHA
                     WEAIDYDLTQFGSTGPDDPNFLPVRITPTRVELSEMFGSVNKRVWRAGTP"
     gene            complement(6015..6248)
                     /locus_tag="AC16PP_04320"
     CDS             complement(6015..6248)
                     /locus_tag="AC16PP_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480882.1"
                     /translation="MSTDTGLLSERVGQRRAIHEYNSVDENRELSPIAGFRHTRAVPA
                     RWNEQICIHHNYGHPDYCTYIHEQADRQLPLAG"
     gene            complement(6253..6432)
                     /locus_tag="AC16PP_04325"
     CDS             complement(6253..6432)
                     /locus_tag="AC16PP_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480883.1"
                     /translation="MAGGNGLVEMERIAREVEPLFGVGIEIWGFDSGKGLPAPRDYRD
                     LPYIWQAAARVLLHG"
     gene            6926..8260
                     /locus_tag="AC16PP_04330"
     CDS             6926..8260
                     /locus_tag="AC16PP_04330"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6480884.1"
                     /translation="MSGSAKFAFTPADKVPDPDLGTGRIPVDRYTDPAFLKLEDERIW
                     SKTWLLAGAACDVAEPGEYFVFENLRESILVTRGDDGEVRAFYNVCQHRGNRLKPSGC
                     GYSEYLRCGFHGWEWNMDGSLKHIPEAHDFPQGTPADELVLQPLRCDTWGGFVWVSMD
                     PDIEPLQDYLGVIPDHLAPYEFEDMVLVSDETVEWNCNWKTSVDIFSETYHVRGVHPE
                     SEYIADDVNVQMDIYGPHSRFIAPMYIPSPRKIGEDRALNQVMVDYLKSEGFPVEDFD
                     GNVTTLRSAVAAHKRAMTNENGLDFSRLTDDQLTADYHYNIFPNVTFNLFGEQMWMFR
                     HRPHPTDPDKMYFDRMIFNRVPKGDVTATANAGAVDLFVELGDEQVEGRPEHVFYRYG
                     EKSSGLLLDQDASCLAGVQQGLHSRGMKGFWISQHEIRIRNFHHWWERYMGI"
     gene            complement(8273..8593)
                     /locus_tag="AC16PP_04335"
     CDS             complement(8273..8593)
                     /locus_tag="AC16PP_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480885.1"
                     /translation="MPTVIIGSALDIVEHCGVPRFLFVDFPLGNPCGRPGDAAMQKRI
                     VRQGLELFETADTGQTTIYCAESWGSDEWRERYMEVTEGNRAELARKGDELRQRRADR
                     QRRP"
     gene            complement(8648..9136)
                     /locus_tag="AC16PP_04340"
     CDS             complement(8648..9136)
                     /locus_tag="AC16PP_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480886.1"
                     /translation="MSTSEKPAEPVPYMRRSRAYYEAQGFERAYRYAHHDAAPFAPLP
                     RPLSECTVGLVTTASTYPRASLEPRRVVSSPTRPAPDKLFADDLSWDKQATHLDDLNS
                     YFPIDHLQTEAAAGFIGTLAQRFHCAPTEYSQRATSEKDAPELHRRLLQDEADVALLI
                     PL"
     gene            complement(9133..11541)
                     /locus_tag="AC16PP_04345"
     CDS             complement(9133..11541)
                     /locus_tag="AC16PP_04345"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HIR6480887.1"
                     /translation="MRRSLGRHRLIMASCFIWLASWPGHASAQQTTSPDAAGSQGTPA
                     EVQPTTDLTSRTQATLTSLDRISGEINELGQLADDAAELDALQIQTDELISITENIDG
                     RGADASFDGSLVKARALAVRAEAVTTALSDHALAMEAKLEEIRQTTAQWQRIQGEITD
                     IPEAISQRIARILATGAGLENRINAKLNETVELQNEALGVLDVARSLQERFDSYGRSR
                     RGQLLEANAAPVWLMKSEHLSSSSEKAGRDFVGTLRRDYATWVETSQAIIGGHFLLLP
                     LLLFLLTKLKATALQSPTGALARPTSTGLLIWMLLGVAIYAGAPVAVRTVFVAIASIV
                     TGFVLVGFLDKRLRAGAIAFLILNVVEDLISGFRVTDALPRLVYLLLALGAIVLVMLA
                     RRPHTLSAMLAWRMPRPILVSGMWTGIALLVLSCIANVAGYVYLSQVLVNGVLASTGV
                     LLVLFAGLTSVSEIIVAVLSLDALNNVRSIANHRYRLNRALRRPLVVLAMFLWAWATT
                     RAFGIDDWVIASIGAIVGAEMSIGSVTLSLQGVLVFVIAVLSAVWASRIVRAVLNYDV
                     LPRMQLPRGVPNTISMTLHYSIILIGLLLGVGFMGIDLSSLAFIVGALGVGIGFGLQN
                     LVNNFVSGLILIFEAPIQVGDTVEVGPLMGKVVQIGIRTSRVRTFGGSEVIVPNGDLV
                     SNQVINWTLSDRSRRLELEVGVAYGSDPERVTEVLRSVVDGDEDVLEDPAPIIMFETF
                     GESSLNFRIFAWIDDFDKGFGMRHRLNVAINRALAEAGLEIPFPQRDLHIKNVPPELG
                     AKPA"
     gene            complement(11686..13029)
                     /locus_tag="AC16PP_04350"
     CDS             complement(11686..13029)
                     /locus_tag="AC16PP_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR6480888.1"
                     /translation="MDLNTLTSFEQRYLWEPDYRQAPPWQRVLVGMARTLMLLFRDIF
                     HGDISLRAMSLVYTTLLSMVPLLALSFSILKGFGVQNQLDETLRAALAPLGEEKALEL
                     TENLITFVNNMNVGVLGAVGLLFLLYTVVSLMQKIEDSFNHTWRIRTPRTFGERFTTF
                     LSAITIGPLLIFTAIALTGSIMSTTVMTHVQEIAVVGPAIQIVVRLLPFVFVIGAFTF
                     LYVFIPNTRVRISAALTGGIVAGVLWELLGFGFASYASGASRYQLIYATFATAIFFLI
                     WLYLNWLILLIGASIAFYRQHPEVIASGSKEVHFSQQLTTRYVLSLLAKIGRRHYEKK
                     KPYTLDELTASYRIPANALEHCLELLVALDVLAETDEPKPSYIPALPLDTTTVAEVLQ
                     RVETYQPPGTYAPALGADEDIVTIEAAGGSGRDRALGGLTLKQLAAGDSSEVVTA"
     gene            13200..14078
                     /locus_tag="AC16PP_04355"
     CDS             13200..14078
                     /locus_tag="AC16PP_04355"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6480889.1"
                     /translation="MSDAQARGLPPDDPTTLITPELIEQYRRDGVICVRNALHPEWLM
                     LLEMGLERIMADPGQQKHLFYRGEPGEFIETIRNFDVAPEIRRLIYDSPIADMIGKLI
                     GSGNIWLYSDEFFVKEGGGCERTPWHQDLPYWPLEGEQIASMWISLDPLPREDCLEYV
                     AGSHQGPMFDGFDPQRVTEDPTLPHYGEGLPPLPDIEADRASFNIVSWEIGPGDVILA
                     HPGVLHGGGATGIADRRRAITVRCYGDDVVYATRPPTRPTVPLTPGLGLKLKPGDPLR
                     HPYYPRVRPLPEHQQV"
     gene            complement(14148..15365)
                     /locus_tag="AC16PP_04360"
     CDS             complement(14148..15365)
                     /locus_tag="AC16PP_04360"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6480890.1"
                     /translation="MPRVLYSNANLLDGEHAARPGSSVIVEDGHILAVGTPESMAAAA
                     YDELIDLEGRTLMPGLVMGHYHAAYRGYGEEGGSQTATAVEQAFFALGNVQIALKCGY
                     TSLISAGTHYNIDVELAQAIDAGEVVGPRLVPCSRNYMPAMSADSAMENEETCMAQGP
                     EGFRKGVLFDLERGVKIVKIFASGGHGAPAGMCMSAEEVKAVVEVAGDYGARVRAHVA
                     GKQQILDCLRAGVTIIDHGDEADEECIAAYLEHDAFVLPSGYVLLKAAELGGNTFGFS
                     EDLKDFESLCRLLPRLVDAGVKLVPGDDFGIWQIPHGAYAEELVCYAEHAGIPPLELI
                     KWSTLHGGEMTGFEGIGTIAPGKVADLLILNDDPSRDIRVLTRPESVQAVLKGGQLVS
                     GELPTPLAAVSAG"
     gene            15611..16432
                     /locus_tag="AC16PP_04365"
     CDS             15611..16432
                     /locus_tag="AC16PP_04365"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6480891.1"
                     /translation="MDLGLEGKKVIITGGSRGLGRAIGETLLREGAVVSFCSRTGRIE
                     PEDEDPRLGGEVRTDGVEQAAEKLAALGEVYGSVVDAADREQLARWVNEAAETMGGID
                     VVVSNTSALGGQPRSPEGWQRQFDVDIMSAVTLWDNAYPFLKASGAGAFVQVSTITAV
                     DYHAYGQACQSYGAFKGALVNYVCQLAYEYMSEGIRANCVSPGPIFVTGGSWDKIQRA
                     LPEYYEENVARQPAGRLGRPEEVADVVAFLASPRASWVTGENIVVDGAFTKYIKY"
     gene            16442..17890
                     /locus_tag="AC16PP_04370"
     CDS             16442..17890
                     /locus_tag="AC16PP_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480892.1"
                     /translation="MQSAQPDGQVLVADARAASEVLHEVLADTAAAFPGALGPMGFPA
                     DPKAFRSTYPEVMPRFEAARLASGDRLAMARHAVGALQRRLVWQDAGGSRPLADALAE
                     PAAPLELLQTDFPGEPGWRPSVVYRGERWEADRLADFGAELLGRGVVTAQASQALAWL
                     ADNVLDAGVLRLTGRKIAVLGGGAEMAPTRLWLEAGADVLWLDVAPPPPSWQDASAMS
                     GRLYWPAGNADLLAQPREILATLIEFAAGRPLDLGLYAYAPGRARELRLTGVMNELVN
                     AMPPELVGSITVLVSPTTPTGLAEPDLAAMRARREARPGWEAALDNLGLLGRGGGCAT
                     MGEVAATRTVVGIQGASYQAAQYLGKVLVAECWAEEGPLGSAHPAPFRVSANTAAITR
                     TRSLGHPVFAAAFGGAAAFGVETLTPRQSRRINGLLALCDWLRPDRPVPGTVRVHGGI
                     HTLPYPLESALRIAAAIGFARSPRLLRGLFGK"
     gene            complement(17946..>18543)
                     /locus_tag="AC16PP_04375"
     CDS             complement(17946..>18543)
                     /locus_tag="AC16PP_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6480893.1"
                     /translation="LASAGYPRESIDTVLCTHLHLDHVGWNTMLEGGQWVPTFPNARY
                     LLERSEFAYVAQEAEAEDVEPWLRDMNRAVMADSIRPVVDAGLIDLVDADHRLCEEVR
                     LMPTPGHTIGHVSVHIASRGESALITGDFIHHPCQLAHPEWSVTTDYDPSDSVSTRER
                     VFAELADGPTLVIGTHWPEPTAGKIASWGDTYQLKTDP"
BASE COUNT         3221 a         5770 c         6176 g         3376 t
ORIGIN      
        1 gaaccccatg gccgacattc cttacgagta ctacgtcgat atcgtgacca agcgatactt
       61 tcatggtgtc gacaaatgcg acatggatat ggtgctggac tgctttcacg aggacgcggt
      121 gctcaccgaa gtgacctcga tgacggtcca cgaagggcgg gacgagggta tccgcaggat
      181 gttcgagggc ctcttcgacg cccacagccg catctggcac ggtaatttcg tccacacggc
      241 ggacgtcgac ggcgaagcca tctgctccca gttctccgtg gaggtcaccc ccaaagagac
      301 cggaacggag ctgcgctacg agaactgcaa ccgcttctat ctggacaacg gcaagttctc
      361 cagagtgttc gtttacatga gcggcgagaa cctgctcaag tgagccgggg cgggaaagga
      421 ggataaccca gtgcaatatc agtcttcgct gagccggtcg gggctcgaga ccctggcgat
      481 cgagagctac ttcggcaacg tggacgccaa gaacctggat gccgtgctcg attgttttca
      541 cgacaacgcc atgatcaccg tgcagaccgc gttcaccacc cacgtcggca aggatgggat
      601 ccgccggatg ttcgtggatt tcatgggagc ctacaaaacc atcatccatc gggatttcac
      661 ctgcaccatc gacgagaaga acggccggat cgccgcctgc ttcacggcgg agctggagga
      721 tcacgacggc aactggacca ccctgcagaa caccaacttc tggcgtgtcc gcggcgacag
      781 gttccaggag gtctacatct acatgagcgg ggagaatccg ctggtgtgag cccctggcgc
      841 ccgggagccc atcgacggcg gaagagccaa gctaccgttg cccgacggcg agcgcgagct
      901 ttccgcgcac gtgcccggcc tgagagcgat ccatggcgag tgctgcttgc tcgaggggaa
      961 acgattcaat ctgagccggc cgcagggcgc cggcttcgat gagccccgtc aggcggctga
     1021 ggtgctggcc gttcggctcc gagaagacgc gcagcccgcg tatgccgcgc tgggcgcata
     1081 gggcctcgtc gggcgcgtcg ttcagcgcgg gcaaggcgcc gccgggcctg accaggtcgt
     1141 agctctcgcc caggacctcg ccgccaacgg tgtcgaagac cacatcgaag cccgctggca
     1201 cttcggaccc catgacctcg cccagatttt cctgccggta gtcgatgatc cggtgtgcgc
     1261 cgagctcgcg gacataggct gcgtttgccg cgctggtggt ggcgaaaacc cgcgcgcccg
     1321 catgggcggc cagctgcacc gcgaggctgc ccacgccgcc ggctgcggcg tggatcagga
     1381 tggtctggcc agcggtcagc cccgcgaact cgaagagcga ttgccagacg gtcaggcccg
     1441 ccagcgggat ggtggaggtc tcaacgtcat cgaggtttgc gggcgcgggc gcgacgaagc
     1501 cagccggcac cgtgacgagc tcggcatacg tgccgccgtg gaccacgggc ttgcggcagt
     1561 aggcgtacac ccggctgccg atctgcattt cagttacccc gtcccccaga ccggcgacct
     1621 ctccggcggc atcccagccc gggatgatgg gaaaccggtg gggaaagatt ccgtcgaaga
     1681 agccctcgcg aatcttccag tccaccgggt tgacgcttgc cgcgtgaatc cggatgagca
     1741 cctctcccga ggcgggctcg ggtgtcggca actcgccgac cgtgagaacc tcggacccgc
     1801 caaaccgctc gtagtagact gcacgcattc aatctgcccc tcttcgaacc aatggagccc
     1861 tcatggagcc gcccaccgaa ccccgcctga tcaagacgga tcctgacccg ttcgagccgt
     1921 tctgcatcgc ccaaggttat caggtgggca acctgatttt cctgtccggc caggcggcgc
     1981 tggatctcca ggggaacgtc gtcggttccg gcgacttcga cctgcaggcc gagcaggcgc
     2041 tgcgcaacgt tcagatgctc ctcgatcagg caggctccgg cctcgacaag ctgatcaagg
     2101 tgaccatcta tctgaccgac atgacgcagt tcccgaagat cgtggagctg cggcgaaagt
     2161 ggttcacgcc gccgtacccg gcggacacca tcgtcgaggt ccgttcgctg gccctgccgg
     2221 agctgatgat cgaaatcgac gccatcgccc tggccggctg aggcgcttcc cctcagcgtt
     2281 gctccagctg tcgggatccg aagaaagctt cgcgcgtttt ctcgtccggg tcgccgatct
     2341 tcatgacttc tgcgagcaga gacgtgcccc gcgccacggc cgggcgctgc gcaatgcggt
     2401 ctgcccagtc ggccacgttg ggaaactcat cgatgtcgat cttgtgcagc tcccggatga
     2461 tcggcatgca ccagggatag gtggcgatgt ctgccagcga gtaggccggc cccccgaggt
     2521 aggcgcagct cgccaggcgc cggtccatca cgccgtagag ccgtcgggtt tcgttgtgat
     2581 agcgctcgat ggcgtagggc accggttcgg gcgcgtagcc ctggaagtgg ccgcactgcc
     2641 cgaacatggg gccgacgttg gcattctgga aaaacaacca ctgcagcatg tcgtacctcg
     2701 ccgccgtgct cgacggccac aggacgcccg tcttctccgc caggtagagc agaatcgcgc
     2761 cggattcgaa cagcgcgtag ggcgtcccgt cgggtccgct gcggtccacg atggcgggga
     2821 tgcggttgtt ggggctgatc tcgaggaagt cgggatcgaa ctgctccccc tcgaggatgt
     2881 tgaccgggtg cagctggtac tcgctgccga tctcctcgag catgatggcg accttgtggc
     2941 cgttgggcgt cggccagtag tagaggtcga tcatcccgtg tcgtgctgtt tctgcgctgc
     3001 cagcacggcc tgcacctgtg cctggcagaa ctcggcgctc gagtcgccga cgaaatcctt
     3061 caggtagtgg gactggaccg agggacgctc aacggcttcg tcgaaccagg cggacagcgc
     3121 cggtcggccg tcgcgccacc tgaaatcggg atggatcgga tccttggcct cgtagaattc
     3181 ggccctgaat cccagatagc tgatcgcatg aagcatagcc gcgtggccga cgtcgaaatc
     3241 gccccagccc cgctcggcgt cccgctccag cttgtccagc ccggcgatga acttgggcca
     3301 cagccactcg aaaaggccgc ggttccactc gctttccggc ttccactgct ccatcaccat
     3361 gaccaccgtc tgctcgaaga tggtgtcgct gagcgccagg cgcgtcaccg cgtcccatcg
     3421 cgccgggccg ggctcggggt acatgcggcg ggccctgctg gtgccgtcga gatattcgca
     3481 ggccgcctgg ctgccgtaga tgacctggcc ttccccggtg accagggtcg gcactttgtt
     3541 cagagggttg agtgctgaga tgtcgtaggc gtcgccctgg tcgatgccct cgttgttctt
     3601 gaagggaaac gtggcgacga aggtgatgtc gtcccagtgg ccgcactcgt gagcggtgac
     3661 cagcaccttg tgaatgaaag tgtggaaggg tgaatagaat atccacatca gcaggttccc
     3721 cctcttcgct accggaccga ccctcacgtc actggggcga tgatgcccga acgttcagcc
     3781 tggcgcagca gttcatttcg cggcagcgat ccgttagcct ttccggccac acctggcgcc
     3841 gcgaggaggg caggggtgaa attcgttggg tacctgatcg gctcggtggt catcctggtc
     3901 gttctgatct tcgtctccca gctcgtcgcg tccgaatcgg cggaggtggt tgtgttgacc
     3961 agccaggggc ccgacggcgg gcaggacacc cggttgtggg tggtcgatcg ggacgggctg
     4021 cagtacctgc gagccagccc tgactccggc tggtaccagc gcctggcggc ggaaccgcag
     4081 gtgctgctcg tccgcaacgg aaacagcgcc gcttaccgtg ccgagatcag aatggccctc
     4141 gccgaggacg tcaatcgtct gatgcgggaa aagtacggct ggcgggacgc gtacatcgag
     4201 tttctcatcg gcgatcggat ggcagcggtg ccggtcgcgc tcattcccga ctgatcgccg
     4261 gccccgcgta ggcacggggc cggctttgag ttctctgcat gtcctgcgaa ccttgccgcg
     4321 gagagattga ggcttgagct tttgcttccc gcgtagtgag atgcggcagt aagtgaggtc
     4381 gtcacgcggc cgtgagttca gccaagcgaa ggagcatttg aggtgaagga tctgaaactg
     4441 ggcctgcaat tgggttactg gggcgctgga cccaaccccg acatggtgaa ggtcgctcag
     4501 gaagcagagc gtctgggcta cgatgcgatt ttcacggctg aggcgtgggg ctcggacgtc
     4561 ttcacgccgc tggcctggat aggggctcac acggagcgga tcaggctcgg gaccggcatc
     4621 gctcagcttt ccgcccgggc gcccaccgcc acggcgatgg ccgccctgac cctcgaccac
     4681 ctttccaagg ggcgcgtgat tctcgggctc ggggtgtcgg gaccgcaggt cgtggagggc
     4741 tggtacgggc gcccgttcgg aaagcccctc gcgcgtaccc gcgagtacgt gagcatcatc
     4801 cgccaggtgc tcgagcgcaa ggagccggtg accaacgacg gggagcacta tcctttaccg
     4861 tacacgggtg agggctcgtg gggacttggc aagccgctga agtccatcac ccacccctac
     4921 cgatccgatc tgccgatctt tcttggcgca gaggggccga agaacgtggc catgaccgcg
     4981 gagatcgccg acggctggct gcccctctac tactcgccct atcggcagga ggtctacgcc
     5041 gaccagatcg cccatgccaa gcccggcttc gagatcatgc agggcgtctc ggtgatcatc
     5101 aacgacgacg tcgaggaagc gttgatcccg ctgaagcgta acctggcgct gtacatcggc
     5161 ggcatgggcg cgaagtccag aaacttccac aacgagctgg tgcaacgcat gggcttcgcc
     5221 gacgcggcgg ccaagatcca ggatcttttc ctctccggtc gcaaggatga cgccgcggcg
     5281 gcagttccga cggagcttgc cgacgagata tccctggtgg gatccgtcga gcggatcaag
     5341 gaccggctgc aggcatggcg ggagacgccg gtgacctcgc tgctggtctc cacccagaac
     5401 atctcgcagc tgagaacgtt tgccgaaatc gtgctcggtt gagcgggtga agctcgccgg
     5461 ccgaatctca ggtgcgtggc aggagaccaa cgttgggtcc agcccctcgc tgacaacacc
     5521 ttcgctgaca acaaatgggg gatcagctcg atgagcgacg ttaaggatga agacagagcg
     5581 gcctttttcg aggccgtcga tgccgcctgc aagaaggcgg tctggtgcgc gcttgcatcg
     5641 gtggccggcg atcggccgag agtgcgcatc gtccatccaa cctgggatgg ggaggtgctc
     5701 tggttcgcca ccggtcccga ctcacccaag gccagacagc tgcgtgagaa cccgcacgtg
     5761 gacattcagt accaggtggc gccgcccgat ttcgtgcacg tgatggtgcg cggaacggca
     5821 gagctggtca ccgatcaggc ggccaagaat catgcctggg aggccatcga ctacgatctg
     5881 acacagttcg gctccacggg tcccgatgat cccaatttcc tgccggtgcg tattacgccc
     5941 acccgcgtag aactctctga gatgttcggg tccgtcaaca agcgggtctg gcgcgcgggc
     6001 acaccgtaga cgcctcagcc ggcaaggggc agttgccggt ccgcctgttc gtggatgtag
     6061 gtgcagtagt ccggatgccc atagttgtga tggatgcaga tctgctcgtt ccagcgggca
     6121 ggcacggcac gggtgtggcg gaaaccggcg atgggggaaa gctcccggtt ctcgtcgacc
     6181 gaattgtatt cgtgaatcgc gcgccgttgc ccgacccgtt ctgaaaggag gcccgtgtcg
     6241 gtgctcatga tgtcatccat gtagcagaac gcgcgcggca gcctgccaga tgtagggaag
     6301 atcccggtag tctctcggtg ccggtagccc tttgccgctg tcgaagcccc atatctcgat
     6361 gccgacgccg aaaagcggtt ccacttccct ggcaatgcgt tccatctcga ccagcccgtt
     6421 gccgcccgcc acgccaggct cgctggatgg ctctggtcgg attcaagaga tgtggtacca
     6481 cgcggtctaa catggtgagg tttcctcggt tgtttgagat aggcgggcag gttccgttcc
     6541 attctcgtca gctgctgctc agtggttgct gagggctcag cacgacccat cttgtccggc
     6601 gcgccgtgtg aggctgtggc gccatgagcc aagcgccgag cccgatcagg acatagaaat
     6661 aggagaacag cgcgttgtag aagtgcgtgg tggtggctgc ggcagtttgt gccgccaatc
     6721 ctgctgactg cgcggcgtcg ctagagggcc tgatctctcg acccatgggt tgagcccagg
     6781 cagctggatt gaagatccga ttatccagta tcgttcctcc taattacctg atggcttttg
     6841 ggttgccagg ggtccggatc acggcggttg gctggggctg cttgcgtctt ggtctccatc
     6901 cccctggctc aacggagacg acatcatgag tggatcggct aaatttgcct ttacccctgc
     6961 agacaaagtg ccggacccgg atctggggac cggacggatt ccggtcgatc gatacacgga
     7021 ccctgctttc ctcaagctcg aagacgagcg catctggtcg aagacctggc ttctggcggg
     7081 tgcggcatgc gacgtggcag agccgggcga gtacttcgtt ttcgagaacc tgcgggagtc
     7141 cattctcgtc acccgcgggg atgatgggga ggtccgcgct ttctacaacg tctgtcagca
     7201 tcgcgggaac cggctcaagc cttctgggtg cggttactcc gagtatctgc gctgcggctt
     7261 tcacggctgg gaatggaaca tggacggaag cctcaagcac attcccgagg cgcacgactt
     7321 ccctcagggc accccggccg acgagctggt gctgcaaccc ctgcgttgcg atacgtgggg
     7381 agggtttgtc tgggtctcca tggatccgga catcgagcct ttgcaggact atctgggggt
     7441 cattccggat catctggctc cgtacgagtt cgaggacatg gtcctggtgt ctgatgagac
     7501 cgtggagtgg aactgcaact ggaagacgtc cgtggacatc ttctcggaga cctatcacgt
     7561 acgcggcgtg cacccggaat cagagtacat cgcagacgac gtgaacgtgc agatggacat
     7621 ctacgggccc cattcccggt tcatcgcgcc catgtacata cccagccctc gcaagatcgg
     7681 cgaagacagg gccctgaacc aggtcatggt cgactatctg aagtccgaag ggtttccggt
     7741 ggaggacttc gacggcaacg tcaccacgtt gcgcagtgcg gtggctgctc acaagcgcgc
     7801 gatgaccaac gagaacgggc tggacttttc caggctgacg gacgatcagc tgaccgccga
     7861 ctaccactac aacatctttc ccaacgtcac cttcaatctt ttcggcgagc agatgtggat
     7921 gttccggcac cggccgcatc ccacggatcc ggacaagatg tatttcgaca ggatgatctt
     7981 caaccgggtg ccgaagggcg acgtcaccgc gacggccaac gcgggcgccg tcgatctttt
     8041 cgtggagctt ggtgacgagc aggttgaggg tcgtccggaa cacgtcttct accggtacgg
     8101 cgagaaatcg tccggccttc tgctggacca ggacgcttcc tgcctggcgg gggttcagca
     8161 ggggcttcac tcgcgaggca tgaagggttt ctggatctcg cagcacgaga tacgcatccg
     8221 taacttccac cattggtggg aaaggtacat gggtatctga agccagtgct gcctatgggc
     8281 ggcgttgacg atcggctcgg cgctgccgca gctcgtctcc cttgcgtgcg agctctgcgc
     8341 ggttcccttc ggtgacttcc atgtagcgct cgcgccactc gtcgctgccc cagctctctg
     8401 cgcagtatat ggtggtctgg ccggtgtctg ccgtctcgaa cagctcgaga ccctgcctga
     8461 cgatgcgttt ctgcatcgcc gcatcccccg gcctgccgca gggattaccc aggggaaagt
     8521 cgacgaacag gaatctcggc acgccgcagt gctccacgat gtccagcgcg gagccgatga
     8581 tcaccgtggg catgccttgc tcctctaagt acctcgcaac cagactcacg gtctggtggc
     8641 agacgggtca caaggggatc aggagggcaa cgtcggcctc gtcttggagg aggcggcgat
     8701 gcagctcggg cgcgtccttc tctgaagtgg cgcgctggct gtactcggtc ggagcacagt
     8761 gaaagcgttg cgccagcgtg ccgatgaagc ccgcggctgc ctcggtctgc agatggtcga
     8821 tgggaaagta cgaattcaga tcatcgagat gggtcgcctg cttgtcccag gacaggtcgt
     8881 cagcgaacag cttgtccggt gcgggcctgg tcgggctcga gaccaccctg cgcggctcca
     8941 ggctggcgcg tggatacgtg cttgccgtcg tgaccagccc gacggtgcat tcgctcagcg
     9001 gcctgggcag aggggcgaag ggtgccgcgt cgtggtgggc gtagcggtag gcgcgctcga
     9061 aaccctgggc ttcgtagtat gcccgggagc gtcgcatgta gggtaccggc tctgccggct
     9121 tttcactggt gctcatgccg gcttggcgcc gagctccggc ggcacgttct ttatgtgcag
     9181 atcgcgctgc gggaatggaa tctcgaggcc tgcctcggca agagcccggt tgatggcgac
     9241 gttcaggcga tgccgcatgc cgaatccttt gtcgaagtcg tcgatccacg cgaagatcct
     9301 gaagttgagc gagctttctc caaaggtctc gaacatgatg atcggtgcgg ggtcttcgag
     9361 gacgtcctcg tcgccgtcca cgacgctgcg caacacttcg gtgacccgtt ccgggtcgct
     9421 gccgtaggcc acgccgacct cgagttccag acgtcggctg cggtccgaga gcgtccagtt
     9481 gatgacctgg ttggagacga ggtcgccgtt gggcacgatc acctccgaac ctccgaaggt
     9541 gcgaaccctg ctcgtccgga tgcctatctg caccactttc cccatcagcg gaccgacttc
     9601 caccgtatcg cccacctgta tgggggcttc gaagatcagg atcaggcccg agacgaagtt
     9661 gttgaccagg ttctgcaggc cgaagccgat acccacgccg agcgcgccca cgatgaatgc
     9721 caggctgctg aggtcgatgc ccatgaagcc cacgccgagc agcaggccga tcaggatgat
     9781 cgagtagtgc agcgtcatcg atatggtgtt gggaaccccg cggggcagct gcattcgggg
     9841 gagaacgtca tagttgagaa cggcccggac gatccgcgaa gcccagacgg cggacagcac
     9901 cgcgatgacg aagaccagca ccccctgcaa tgacagcgta accgagccga tcgacatctc
     9961 ggcgccgacg atcgcgccga tggaggcgat gacccagtca tcgatgccga acgcgcgggt
    10021 cgttgcccat gcccacagga acatggccag cacaacgagc ggtctgcgca acgcccggtt
    10081 gaggcgatag cggtgattgg cgattgaccg tacgttgttc agtgcgtcca ggctcagcac
    10141 ggcaacgatg atctcggaga ccgacgtgag ccccgcgaac agcaccagca gcaccccggt
    10201 gctggccagg acgccgttga ccaggacctg cgacaagtac acgtacccgg cgacgttggc
    10261 gatgcagctg agcaccagaa gggcgatgcc cgtccacatc ccgctgacca ggatcggacg
    10321 gggcatgcgc caggcgagca tggcgctcaa cgtatggggt cgtcgggcca gcatcacgag
    10381 aacgatggcg cccaaggcaa ggagcaggta gacgagacgt ggcagcgcgt cggtcacgcg
    10441 gaagccgctg atcagatcct ccaccacgtt cagtatcagg aatgctatcg ccccggctcg
    10501 cagtcttttg tcgaggaaac cgacgaggac gaacccggtc acgatcgagg caatcgccac
    10561 gaagacggta cgcacggcga ctggcgcccc ggcgtagatc gccacgccca aaagcatcca
    10621 gatgaggagc ccggtcgacg tggggcgggc cagagcgccc gtcggggatt gcagtgccgt
    10681 cgccttgagc ttcgtaagca ggaacagcag cagtggaagc aggaggaagt gaccgccgat
    10741 gatcgcctgg gaggtttcta cccaggttgc gtagtcgcga cgcagcgtgc cgacgaagtc
    10801 ccgacccgct ttctccgaag agctgctgag atgttcggat ttcatcagcc agaccggggc
    10861 cgcattcgct tccagcagct gcccgcgccg ggaccgcccg tagctgtcga agcgttcctg
    10921 cagcgaccgg gcgacgtcga gcaccccgag cgcctcgttc tggagctcca cggtttcgtt
    10981 cagcttggcg tttatccggt tctccaggcc tgcaccggtc gccaggatcc tcgcgatacg
    11041 ctggctgatg gcttccggaa tgtccgtgat ctcgccctgt atccgctgcc actgggccgt
    11101 cgtctggcgg atctcttcga gtttggcttc catggcgaga gcgtggtccg acagggcggt
    11161 ggttaccgcc tctgctctga cggcgagcgc tcgggctttc accagcgaac cgtcgaacga
    11221 ggcgtctgcg cctctcccgt cgatgttttc ggtgatgctg atcagctcat cggtctgaat
    11281 ctgcagggcg tccagctctg cggcgtcgtc ggcgagctgg cccagctcgt tgatttctcc
    11341 tgagatgcga tccagggacg tcaacgttgc ctgggtgcgg ctggtgaggt ccgtggttgg
    11401 ctggacttcc gcgggcgtcc cctgggatcc ggcggcatcc gggcttgtgg tctgctgcgc
    11461 tgacgcgtgg ccgggccaac tagcgagcca gatgaagcac gacgccatga tcaatctgtg
    11521 gcgacccagc gatcgacgca tcaccatggc cctttgggga aagtggcgaa gtttaggggc
    11581 gaagcttcct ggtctcaacc ggtacccagg cgcgcacgtt gacgagccga tccacgggcc
    11641 aatcgcgatc gcgcgggggc tccttccagg gcgggaggat cctgatcagg ccgtgacgac
    11701 ttcgctcgaa tcgccggctg ccagctgctt gagcgtgagc ccgccgagcg cacgatctct
    11761 gccggaaccg ccggctgcct cgatcgtcac gatgtcctcg tcagcgccca gggcgggcgc
    11821 gtaggttccg ggtggctggt atgtctccac ccgctgaagc acctcggcga cggtggtggt
    11881 gtccagggga agggcgggaa tgtaggaagg cttgggctcg tccgtctccg cgagcacgtc
    11941 cagcgccacg agaagctcga ggcagtgctc cagggcattg gctgggatgc gatagctcgc
    12001 cgtcagctcg tcgagggtat agggcttctt cttctcgtaa tgccggcgac cgatcttggc
    12061 gagcagcgat agcacgtagc gggtggtcag ctgctggctg aagtgcacct ccttcgagcc
    12121 cgaggcgatg acctccggat gctgccgata gaaggcgatg cttgctccta tgagaagaat
    12181 cagccagttc agatacagcc agatgagaaa gaatatggcc gtagcaaacg tggcgtagat
    12241 cagctggtag cgggaggcgc cgctggcgta ggaggcaaat ccgaagccca ggagctccca
    12301 gaggacgccg gcgacgatgc cgccagttaa tgccgccgag attcgcactc tcgtattggg
    12361 gatgaagacg tacaggaagg tgaacgcgcc gatgacgaag acgaacggca gaagcctgac
    12421 gacaatctgg atggccgggc cgaccacggc gatctcctgg acgtgggtca tcacggtggt
    12481 gctcatgatc gagcccgtca gggcgatggc agtaaagatg agcagcggcc cgatggtgat
    12541 ggcggagagg aaggtggtga aacgttcccc gaacgttctg ggcgtgcgaa tgcgccaggt
    12601 gtgattgaat gaatcctcga tcttctgcat cagcgaaacc acggtgtaga gcagaaacag
    12661 cagaccgacc gcgcccagca cgcccacgtt catgttgttg acgaaggtga tcaggttttc
    12721 cgtcagctcg agggcctttt cctcgcccag aggcgccagc gctgcacgta gagtctcgtc
    12781 gagctgattc tggaccccga atcccttgag aatcgagaag ctcagcgcga gcagcggcac
    12841 catggagagc agcgtcgtgt acacgaggct catggctctc aggctgatgt caccgtgaaa
    12901 gatgtcccgg aacaggagca tgagcgttcg ggccatgccc accagcacgc gctgccatgg
    12961 gggagcctgc cgatagtccg gctcccagag atagcgctgc tcgaacgatg tgagagtgtt
    13021 gagatccaag tgcgtccccc tgcccgtgga gtgaagggaa agggatttcc cgggtgccgt
    13081 caaggcccct cgcccaggcg ggtgcgccca tacgacgcgg ccgcgccgcg cgctgcttag
    13141 ccgtagcgag gtttgccggg ctgggctaga ttagggcaaa gggtttctgg agagggagaa
    13201 tgtcagacgc gcaggctcgc gggctaccgc cggacgatcc caccacgctg atcacgccgg
    13261 agctgatcga gcagtatcgg cgcgatggcg tcatctgcgt tcgaaacgcc cttcatccgg
    13321 agtggctgat gctgctggag atgggtctcg agcgcatcat ggccgacccc ggccagcaga
    13381 agcatctgtt ctatcggggc gagcctgggg aattcatcga aaccatacgc aacttcgacg
    13441 tggcgcccga gatccggcgg ctcatatacg actcgcccat tgccgacatg atcggcaagc
    13501 tcatcggttc cggaaacatc tggctttact cggacgagtt cttcgtgaag gaaggcggcg
    13561 ggtgtgagcg gacgccgtgg catcaggatc tgccgtattg gccgctggaa ggagagcaga
    13621 tcgcctcgat gtggatctct ctggatccgc tgccccggga agattgcctg gagtatgtgg
    13681 cgggctccca ccagggcccc atgttcgatg gtttcgaccc ccagcgggta acggaggacc
    13741 cgacccttcc gcattacggc gaggggctgc ccccgctgcc ggacatcgag gcggatcgag
    13801 ccagcttcaa catcgtctcc tgggaaatcg gtcccgggga tgtgatcctc gcgcatccgg
    13861 gcgttctgca tggtggcggt gcgaccggga tcgcggatcg tcgcagagcg atcaccgttc
    13921 gctgttacgg ggacgacgtc gtctacgcca cccggccgcc cacgcggccc acggtgcccc
    13981 tcacgccggg cctcggtcta aagctgaaac cgggagaccc gctgcgccat ccctactatc
    14041 cgcgggtgcg gccgctgccc gagcaccagc aggtctgagg agggatcagc gggcgcatcg
    14101 acccctgcgg gtccgctcgt tgcgctctgc ccgtgtgcgg cggatgccta tcctgcggat
    14161 accgcagcca gcggggtagg cagctccccg ctcaccagct gcccgccttt cagcactgcc
    14221 tgaacgctct caggccgcgt cagcacgcga atgtcccgcg acggatcgtc gttcaggatc
    14281 agaaggtcgg ccacctttcc cggtgcgatg gtcccgatgc cctcgaagcc cgtcatctcg
    14341 ccaccgtgca acgtgctcca tttgatcagc tccagcggcg ggatgccggc gtgctccgcg
    14401 tagcagacga gctcttccgc gtaagcgccg tggggaatct gccagatgcc gaagtcgtcg
    14461 ccggggacca gcttgacgcc ggcatcgacc aggcggggca acagtctgca caggctctca
    14521 aagtccttga ggtcctcgct gaaaccgaac gtgttgccgc ccagctcggc tgccttgagc
    14581 agcacgtagc cgctgggcaa cacgaaagcg tcgtgctcca ggtaggccgc tatgcattct
    14641 tcgtccgcct cgtcaccgtg atcgatgatg gtgaccccgg cacgcaggca atcgagtatc
    14701 tgctgcttgc cggcgacgtg agctcggacc ctggcgccgt agtcgccggc gacctccacc
    14761 acggctttga cctcctcggc gctcatgcac atgcccgccg gcgcgccgtg gccgccgctg
    14821 gcgaagatct tgacgatctt cacaccgcgt tcgaggtcga acagcacgcc ttttctgaac
    14881 ccttccgggc cttgggccat gcacgtctcc tcgttctcca tggcgctgtc agcactcatg
    14941 gccggcatgt agttgcggct gcagggcacc agccgcgggc cgaccacctc gcccgcgtcg
    15001 atggcctggg cgagctcgac gtcgatgttg tagtgggtgc ccgcgctgat caggctggtg
    15061 tagccgcact tcagcgcaat ctgcacgttg ccgagcgcga aaaacgcttg ctcgacggcg
    15121 gtggccgtct ggctgccgcc ctcttcaccg tagccgcgat acgcggcgtg atagtggccc
    15181 atgacgagcc cgggcatgag cgtgcggccc tcgagatcga tcagctcgtc gtacgcggcg
    15241 gccgccatgg actccggggt gccgacggca aggatgtgcc cgtcttccac gatgaccgag
    15301 gacccgggtc tggcggcgtg ctcaccgtcg agcaggttcg cattgctgta cagaactcgt
    15361 ggcatgttgt ccctcccgtg gcggagatgg tcggtgcaac ggccgatggg tgatgagcct
    15421 cggcacgact tgcccccaac acccaacagt atcactggcc cggaccagac gcatccggag
    15481 atcgcctgtg agttcggcgt gttcgccgga tgactttggg gaacactcca accggtgaga
    15541 gtcgggtgca cggcagcggc gctggccggg ttcgctatac ttgcctgatc gggttttggg
    15601 ggtgtgagtc atggatcttg ggctcgaagg aaagaaagtc atcatcaccg gcggctcgcg
    15661 gggtctgggt cgcgccattg gcgagacgct cctgcgcgaa ggagccgtcg tgtcgttctg
    15721 ttcccgcacc gggcgtatcg aaccggaaga cgaagatccg cgccttggcg gggaggtccg
    15781 gacggacggc gtcgagcagg cggcagagaa attggctgcc cttggcgagg tgtacgggtc
    15841 cgtcgtggac gccgccgacc gcgagcagct cgcgcgctgg gtgaacgaag cggccgagac
    15901 catgggcggc atcgacgtgg tggtttcgaa caccagcgcg ctcggggggc aaccgcgttc
    15961 gccggaagga tggcagcggc agttcgacgt ggacatcatg tccgcggtaa cgctgtggga
    16021 caacgcctac ccgttcctca aggcttcagg agcgggcgcg ttcgtgcagg tctcgacgat
    16081 taccgccgtg gactaccacg cttacgggca ggcgtgtcag agctacggcg ctttcaaggg
    16141 agcgctggtc aattacgtgt gccagcttgc ctacgagtac atgagcgaag gcatccgcgc
    16201 caactgcgta tccccggggc cgatcttcgt caccggcggt tcctgggaca agattcagcg
    16261 ggccctgccc gagtattacg aggagaacgt ggcgcgtcag cccgcgggtc ggctgggcag
    16321 gccggaggag gtggccgacg tggtggcctt cctggccagc cccagggcgt cctgggtcac
    16381 gggggagaac atcgtcgtgg acggtgcctt caccaagtac atcaagtatt agccgttcgc
    16441 agtgcagtcc gcacaacccg atgggcaggt cctggtcgca gacgcgcgcg ctgcgtccga
    16501 ggtgctccac gaggtgcttg ccgacaccgc cgcggccttt cccggcgccc tgggtccgat
    16561 ggggtttccc gcggatccca aggcattccg ttccacgtat cccgaggtga tgccccgttt
    16621 cgaggcggcc cgtctggcca gcggcgatcg ccttgccatg gcacgccacg cggtcggcgc
    16681 cctgcaacgg cggctggtct ggcaggatgc cggcggtagc cggcccctgg ccgacgcgct
    16741 ggcggagccg gctgcgccgc tcgagctcct gcagaccgac tttccggggg agcccggctg
    16801 gcggccgagc gtggtgtatc ggggagagcg ttgggaagcc gatcggctcg ccgatttcgg
    16861 cgcggagctg cttggccgcg gcgtcgtgac cgcgcaggcg tcccaagccc tcgcctggct
    16921 ggctgacaac gtgctggatg ccggagtgct gaggctgaca ggacgcaaga tcgcggttct
    16981 cggcggcggc gctgagatgg cccccacgcg gctttggctc gaggcagggg ccgacgtgct
    17041 ctggctcgac gtggcgcccc cgccgccgag ctggcaggac gcgtcggcca tgtccggtcg
    17101 actgtactgg ccggccggaa acgccgatct gctcgctcaa cctcgtgaaa tcctcgctac
    17161 gctgatcgag tttgccgccg gcaggcccct cgacctcggc ctgtacgcct atgccccggg
    17221 gcgggcgcgg gaactgcgac tcacgggggt catgaacgag ctggtgaacg cgatgccgcc
    17281 ggagctggtg ggcagcatca ccgttctggt ctcgccgacc acgccgaccg gcctcgccga
    17341 gccagaccta gccgccatgc gcgcgcgcag ggaagcccgc cccggctggg aagccgcgct
    17401 cgacaatctc ggcctgctgg gaagaggggg aggctgcgcc accatgggag aagttgcggc
    17461 aacccgcacg gtggtgggca ttcagggagc cagctatcag gcggcccagt acctgggcaa
    17521 ggtgctcgtg gcggaatgct gggccgagga aggcccgctg ggcagcgctc accccgcacc
    17581 gtttcgggtg tccgccaaca ccgcggcgat cacgcgcacg cgctccctgg gtcacccggt
    17641 ctttgccgcg gcgtttggcg gggcggcggc attcggcgtg gagacgctga ctccccgcca
    17701 gtcgcggcgc atcaacgggc ttctggccct ttgcgactgg ctgcgcccgg accggcccgt
    17761 gcccggcacc gttcgggtgc acggcggcat ccacacgctg ccgtatccgt tggagtcggc
    17821 gctgcgcatc gcggccgcca tcggctttgc gcgctcgcct cggctgctgc gaggcctgtt
    17881 cgggaagtga tcggctcggg gctcgatgtt ccggtctcgg gccgttgggg tcggggagcg
    17941 gcagctcagg gatccgtctt gagctggtag gtgtcgcccc agctggcgat tttcccggcg
    18001 gtgggttccg gccagtgggt gccgatcacc agggtagggc cgtcggccag ctcggcaaag
    18061 acccgctctc tcgtgctcac ggaatctgaa gggtcgtagt cggtcgtcac ggaccattcc
    18121 ggatgggcga gctgacaggg gtggtggatg aaatcgccgg tgatcagggc ggactcgccg
    18181 cgggaggcga tgtgcacgct gacgtggccg atggtatggc cgggcgtcgg catcaaccgc
    18241 acctcttcgc acaggcgatg gtcggcatcc accagatcta tcaggccggc gtccaccacc
    18301 gggcggatgg agtccgccat cacggctcgg ttcatgtcgc gaagccaggg ctcgacgtct
    18361 tccgcttctg cttcctgggc cacgtaggcg aactcgctgc gttcgagcag gtagcgggcg
    18421 ttggggaagg tcggcaccca ctggccccct tcgagcatgg tgttccaacc cacgtgatcc
    18481 aggtgaagat gcgtgcagag caccgtatcg atcgactctc gcgggtagcc tgccgaggcc
    18541 aga
//
LOCUS       DBNHKV010000134        15633 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1931448, whole genome shotgun sequence.
ACCESSION   DBNHKV010000134 DBNHKV010000000
VERSION     DBNHKV010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 15633)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15633)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15633
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1931448"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="AC16PP_04380"
     CDS             complement(<1..191)
                     /locus_tag="AC16PP_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIR6480894.1"
                     /translation="MARINLLPWRDWERERRQKEFMANLGGVLIIAVVIVFAAGFMLD
                     RSVEHQDERNRFLQTHLDEL"
     gene            complement(191..1255)
                     /locus_tag="AC16PP_04385"
     CDS             complement(191..1255)
                     /locus_tag="AC16PP_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HIR6480895.1"
                     /translation="MFGLFKNKSNVLLGLDISSTSVKLLELSKSNDRFRVESYAVEPL
                     PLNAVVEKNISDVEGVGEAIKKVVSRSKSSCRRAAVSVAGSAVITKTIEMDASLSDED
                     MENQITVEADQYIPYPLDEVALDFEVQGLSERNPDQVEVLLAACRKENVELREAALDV
                     GQIKPGVVDVEAHAMQRAFDLVKPRLGGNPEELVVAIIDIGATMTTLSVLVDGKSVYT
                     REQLFGGKQLTEEIQRRYSLSFDEAGLAKKQGGLPDDYQSEVLEPFKEAVLQQVTRSL
                     QFFFSSSQFDDVDYIVLAGGTASIDGLAEMVEGKLGTPTIIANPFADMAFAGRIDKDQ
                     LMNDAPALMIACGLAMRSFD"
     gene            1434..3851
                     /locus_tag="AC16PP_04390"
     CDS             1434..3851
                     /locus_tag="AC16PP_04390"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR6480896.1"
                     /translation="MLSLAGAFLYLDPQIPRAETYRQVKLETPLRVYSSDEKLIAEFG
                     ERRVIPLKLEEAPPLFVAALLDTEDKRFYDHSGIDYISLLNDTVELALNREIRSGAST
                     ITMQLARNISFSLEQTFIRKFKEMLLALQIEQELGKDEILELYMNVVPFGKRAYGAQA
                     AALTYYGKRLDELNLAQLAMLAGIPQAPTAGNPINGPARAVRRRNVVLRRMLDQGSIS
                     QAAFDEAIAAPITASLHDRALDVGAPFPAEWVRAELINRYSDLYTGGYQVYTTIVADL
                     QQTATRSLRAGLKSYDRRHGYRGPEQSLGALTEEEYLQALREARSYADLDPAVIVSVD
                     EQGADALLGDGSRGRIAMAEMSWARRYLDVDRRGPPPERPSDVVQVGDVVRVEAIAEP
                     AEAGEAESAGDDPEAEPAPAWRLAQLPDIQGALVSMDPRTGAVLALEGGLDFAMNQYN
                     HALQAARQPGSGFKPFVYSAALDRGVTPASIFMDAPLVFDDQNLESQYRPDNDNNRYN
                     GPTRLREALYRSINLVSMRVLLEVGAGSVMDHVRKFGFDTSTFPRNLQLAIGGGTMAV
                     TPLQMATAYAVFANGGWQIEPHIVDRVVSLDGETVYQASHPRVCDPCDQDPATDLDSE
                     EPANLEDLLAEADDAPIEAERVVDERNTYIMNDMLKDVIRVGTGRRARTLERSDIAGK
                     TGTTNDAADTWFNGYSPDVVTTVWVGFPDHRPLGATEYGSNTPLPIWIEFMSQALANK
                     PERVPAQPAGVVTMKIDRDSGELAGSQQTDAIFELFLAEHTPQPPPSAAAGPSEPGTD
                     LKPMDIF"
     gene            complement(3867..5351)
                     /locus_tag="AC16PP_04395"
     CDS             complement(3867..5351)
                     /locus_tag="AC16PP_04395"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR6480897.1"
                     /translation="MRRRLLRSGYPAPVRQSLAQVGRLIARRRPQLRLQLCALLLPGL
                     VLSGMAVAGPGEKVYKEMLANDQIYQDQEWQAYVNQIGRRLLAVSPDAGKDYHFYILD
                     NPTVNASAFPDAYIFVNRGLIAYLRSEDELAGVIGHEIGHVVGRHARRSNMMGFFGNV
                     AGFIGSIMTGTGAIADMGNTVTQTLRSGYGREFELEADEYGGEFLARAGYNPLAMIDV
                     IHVLKDHSLFAKNVLGQPAVYHGLFSTHPKNDKRLHDAVQKSQHIFPDELREPERDFW
                     EMIDGMVYGDEAATGLIKGETYYHGGLRVVVQFPQGWDVTNTASEVLGRAPGGTPDAF
                     ITVQRLNPAESEQTPEEYVKETLKRDDVTDGESLEISGYSAFIGTVEVASGNAQARRI
                     AVIYKDNGVYLFKGEVGPVGDVNAFEPQWRATLESFRAMTAADLMVANSQRLQVVVAN
                     PGDSYDKLARKVSIKSYPAETLRVLNGDHPIGEPRAGDYIKIVQ"
     gene            complement(5516..5731)
                     /gene="rpmE"
                     /locus_tag="AC16PP_04400"
     CDS             complement(5516..5731)
                     /gene="rpmE"
                     /locus_tag="AC16PP_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007350412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR6480898.1"
                     /translation="MKADIHPEYNQIKATCSCGNVIETCSTLEGDIHLDVCSACHPFY
                     TGKQKILDAGGRVERFRKRFGNRSVSS"
     gene            5851..8022
                     /locus_tag="AC16PP_04405"
     CDS             5851..8022
                     /locus_tag="AC16PP_04405"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004067.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HIR6480899.1"
                     /translation="MNPLTQTNVDVVRVAVAKPLHQLFDYAVPPDMTPPVAGARVVVP
                     FGRQRLTAICVERNPDDAHAQLKPLLSVLDDTAAIPEELRALAGWLCDYYQHPLGEVL
                     ATMLPAEARRGHELTIVRPEQWTATGTRSDLARAPRQQALMEALAAAGGTLSASALAE
                     AGFSRALIRAAAGKGLITRTEAATGFTLGIDEQPPQLSDEQAGVLSDLLARTDCFAPS
                     LLEGITGSGKTEIYLQLIDNVRKAGHQVLVLVPEIALTPQTVGRFERRFGSGGGVASL
                     HSNLGDQERLQTWLKCRSGEVSVLIGTRSAVFAPFAKLGLIVVDEEHDGSFKQQDGLR
                     YSARDVAVKRAQTLDIPLLLGSATPSLESLNNAMAGRYAHLRLRHRAGGARPPRFHLL
                     DIRGQSLDDGLSQDLLRIVGQHLNRGGQALVFLNRRGYAPTYLCPRCSWQAACGHCEM
                     RLTLHLSPRALLCHHCGHQRPLPSACPDCGHAELIAVGAGTQRTEAGLAKRFPGIPLY
                     RVDRDTTRSQRRMEAQFEAIHKGDPAILVGTQMLAKGHHFPKVTVVAVVNADGGFLSA
                     DYRAPERTAQLITQVAGRAGRAEDPGEVWIQTYQPDGVLLRSLIDEGYDGFARRELAI
                     RKSSGLPPFRPMALLRAESRDAESARNFLTSVRDRQGSPLEVLGPAPAPIARVANRLR
                     FQLMVMADSRRELHAGIRPIRDLTAPGTIRWSVDIDPYDTF"
     gene            8100..8666
                     /locus_tag="AC16PP_04410"
     CDS             8100..8666
                     /locus_tag="AC16PP_04410"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR6480900.1"
                     /translation="MANDFANRLNPPTTRRPKARPPSATRRKERRFSGSSFASGLLIG
                     GACMAVALNLPDPQPERPPAAATAASAPPGSVTFEFDKDLRDAFVEADPGQYETDAGH
                     DGSRPVSYLIQAASFPVAADADSLQAELAQMGLAAQSVPARLNAGTWFRVTVGPFDSQ
                     LKANRALTQLREIDLDAFMIQQRLPEPR"
     gene            8777..9319
                     /gene="hslV"
                     /locus_tag="AC16PP_04415"
     CDS             8777..9319
                     /gene="hslV"
                     /locus_tag="AC16PP_04415"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014754676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HIR6480901.1"
                     /translation="MADIHGTTIVSVRNDRQVAIGGDGQVSMGQTIMKGNARKVRRLH
                     DDKVLAGFAGGTADAFTLFERFEGVLDKYHGNLTRAAVELAKDWRSDRALRRLEALLV
                     VADREATLLVSGTGDVIEPEAGVIAIGSGGPFAQAAARALNENTELDPADIVEKSLSI
                     AADICVYTNHHRTIEVLDLT"
     gene            9325..10668
                     /gene="hslU"
                     /locus_tag="AC16PP_04420"
     CDS             9325..10668
                     /gene="hslU"
                     /locus_tag="AC16PP_04420"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019650789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HIR6480902.1"
                     /translation="MTPREIVHELDKHIIGQDDAKRAVAIALRNRWRRMQLEEEMREE
                     ISPKNILMIGPTGVGKTEIARRLARLANAPFIKVEATKFTEVGYVGRDVESIIRDLAD
                     VAVKMLREEQMDRVMHQAEDRAEERILDALLPGPKPTGSQTIGFTQESSEDEGDSTTR
                     QLFRKRLRQGELDEKEIEIETDAAQVGVEIMAPPGMEEMTSQLQSMFSNLGGGRKRSV
                     RLKIKDALRKLKEEEAAKLVNEDEIKARAVETVEQMGIVFLDELDKVAKRSESAGADV
                     SREGVQRDLLPLIEGCTVSTKYGMVKTDHILFIASGAFHLAKPSDLIPELQGRLPIRV
                     ELAALSAGDFKRILVEPNASLTEQYQALLGIDGLSLEFTDDGIERIAELAWQVNESTE
                     NIGARRLHTVLERLLENVSFTATESAESTVVIDAVYVDAQLSELAQDRDLSRYIL"
     gene            10685..11098
                     /locus_tag="AC16PP_04425"
     CDS             10685..11098
                     /locus_tag="AC16PP_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase family protein"
                     /protein_id="HIR6480903.1"
                     /translation="MQRPKQITYRRQSKVLVLSFEDGDAEIPAELLRVYSPSAEVRGH
                     SAAERKLQTGKKHVGIKSLEPVGNYAIRITFDDGHDTGLYGWDLLRDLAENADGYWKS
                     YLDELKAANASRLPTIPVGHWTPASQPAVPPEQDQ"
     gene            11095..11838
                     /locus_tag="AC16PP_04430"
     CDS             11095..11838
                     /locus_tag="AC16PP_04430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008096122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6480904.1"
                     /translation="MSDQNSVDFGSRKVSPDEKTRLVGGVFASVAERYDVMNDLISLG
                     THRLFKRMTVELSGVREGMRVLDLAGGTGDLSALFSGAVGPSGVVVLADINARMMDVG
                     RNRLLDQGCANVSFCQAAAEALPFADDSFDCVSIGFGLRNFTDKRVALEELNRVIAPG
                     GVLLILEFSRPENAFLEGAYGTFQSLWPGLGKLVAGDARSYQYLIESIRNHPPQKALK
                     LMLEDAGFAAVEYHNLLGGIAAIHRGVKP"
     gene            11835..12473
                     /locus_tag="AC16PP_04435"
     CDS             11835..12473
                     /locus_tag="AC16PP_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HIR6480905.1"
                     /translation="MSTADVFESALVELVNRAANATLELDPKAASRLAAAEGHRARLI
                     AALPGGREQSFTLSVVGGRMAFTPHAEAEPNVVVRGSVPALLSMLAGGRSAGPVTIEG
                     DVSVLEELAAIFKGFAPDLAEPLSKLVGETSADGILNAAEAGLALLKSAAEATGESLR
                     RGVGERFATRTRVNVLLDRLEDARERSDRLAARIGNLESARGSHQRPEPPTP"
     gene            12470..14134
                     /gene="ubiB"
                     /locus_tag="AC16PP_04440"
     CDS             12470..14134
                     /gene="ubiB"
                     /locus_tag="AC16PP_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HIR6480906.1"
                     /translation="MSALRRLFRVLWIVSRYRLDTLIDADQEGAALPRRYAWLLRASP
                     ARLLPVGDEPRGRRLLLALEQLGPVFVKFGQLLSTRRDLIPLDIADELSALQDRVPPF
                     PGEQAVRIIEDALGQPISEAFRSFEIEPMASASVAQVHAAILPDGKSVVVKVVRPDIA
                     DVIRADLELLRAAAEILENASEEARRLHLKQVVKDYEQTILGELDMQKEADNTARLRS
                     NFAESELLYVPRVYWRWCRSNILVIERIHGIPISDVAELKARGTNMRRLAEKGVETFF
                     KQVFVDNFFHADMHPGNIFVDVTDPEDPRYIAVDCAIIGSLNQEDQDYLARNLLAFFN
                     RDYAEVARLHLESGWVPDETDPEEFERVIREVCEPIFAKPLNEISFGQFLVELFETAS
                     AFHMEIQPQLVLLQKTLLYIEGLGRELYPELDLWETAKPFMESWMAERVGPAAALREL
                     AERAPEILAELPRLPGMVVTASRQIRHLERMVDRQNRELHELEARVGRLARGVRGRRV
                     AGAALLVLASVLLWGPIAESLSGSGDLGTVAGLASAAIASLLLLKA"
     gene            14138..14485
                     /locus_tag="AC16PP_04445"
     CDS             14138..14485
                     /locus_tag="AC16PP_04445"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HIR6480907.1"
                     /translation="MTDSKDIVEDLIEVLESRRSADPGSSYVASLYADGLNRILEKVG
                     EEATEVVLAAKDATSSDRDDALVHEVADLWFHTLVMLVHLNQDPRWVFETLRERFGIS
                     GHEEKSSRNAGGP"
     gene            14563..14796
                     /gene="tatA"
                     /locus_tag="AC16PP_04450"
     CDS             14563..14796
                     /gene="tatA"
                     /locus_tag="AC16PP_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIR6480908.1"
                     /translation="MFGLGMTELIVILIIVVLIFGPKRLKTIGSDLGNAIKGFRKAVT
                     EEEAKAAPAEEPRVIEGEVNAAQKTETKQDSSV"
     gene            14799..15137
                     /gene="tatB"
                     /locus_tag="AC16PP_04455"
     CDS             14799..15137
                     /gene="tatB"
                     /locus_tag="AC16PP_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016395362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HIR6480909.1"
                     /translation="MFDIGFPELVLIAVVALLVIGPERLPEALRTLGLWLGRMRRSFT
                     AVKTEIEKEIGMDDVRRQLHNEAIMEEMKRIEGEVRGTADSPAGDAQTEAAGNPPSNE
                     TSATADNGGR"
     gene            15124..>15633
                     /gene="tatC"
                     /locus_tag="AC16PP_04460"
     CDS             15124..>15633
                     /gene="tatC"
                     /locus_tag="AC16PP_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR6480910.1"
                     /translation="MAGAEDSDNRLSHDEQVDADEQPFLDHVIELRSRILRGLLVVLL
                     LFFPTYYYANDLYELVAAPLLAHLPGGGKMIATEVASPFLTPFKLSVFTAVFIGMPFL
                     LHQMWAFISPGLYLHEKKFAVPVLLSSMVLFYGGVAFAYFLVFPLVFQFMAAVTPEGV
                     TMMTDINRYL"
BASE COUNT         2858 a         5086 c         4982 g         2707 t
ORIGIN      
        1 agttcgtcca gatgcgtctg caggaaccgg ttgcgttcgt cctggtgctc cacggagcga
       61 tcgagcatga accccgcagc gaacacgatc accactgcga tgatcagcac cccgcccagg
      121 ttcgccatga attccttctg gcggcgttcg cgctcccagt ctcgccaagg tagtaagttg
      181 atacgggcca tcagtcgaag ctcctcatcg ccaggccgca ggcaatcatc aatgccggcg
      241 cgtcgttcat taactggtcc ttgtcgatgc ggcctgcaaa ggccatatcc gcaaacgggt
      301 tggcaataat ggtcggcgtg ccgagcttgc cttcgaccat ttcagccagc ccgtcgatcg
      361 atgccgtgcc gccggcgagg acgatgtagt ccacgtcgtc gaactggctg gacgaaaaga
      421 agaactgcaa tgatcgcgta acctgctgca acaccgcttc cttgaagggt tccagcacct
      481 cgctctggta gtcgtccggg agcccgccct gcttctttgc cagaccggct tcgtcgaacg
      541 aaaggctgta gcgcctctga atttcttcgg tcagctgctt gccgccgaag agctgctcgc
      601 gtgtgtagac cgatttgccg tctacgagca ccgagagcgt agtcatggtg gcgccgatgt
      661 cgatgatggc gaccaccagc tcctcggggt ttccgccgag acgcggcttg accagatcga
      721 aggcacgctg catcgcgtgc gcctcgacgt ctacgacgcc gggcttgatc tgaccaacgt
      781 ccagcgcggc ttcgcgcaac tcgacgtttt ccttgcggca agcggcgagc agcacttcca
      841 cctggtccgg gttgcgctcc gagaggcctt gcacctcgaa gtcgagagca acttcgtcca
      901 gggggtacgg aatgtactga tccgcttcca ccgtgatctg gttctccatg tcctcgtcgg
      961 acagagaggc gtccatctcg atggtcttgg tgatgacggc cgatccggcg acagagacgg
     1021 ccgcacggcg gcagctgctc ttggagcggc tcacgacctt cttgatcgct tcacctacac
     1081 cttcgacgtc gctaatattc ttttcgacca ccgcattgag cggcaggggt tctaccgcgt
     1141 aggattccac gcgaaaccgg tcgtttgatt tagacagctc tagcaacttg acggaagtgg
     1201 agctgatatc cagcccaagc agaacgttgg acttgttctt aaagaggcca aacacggtaa
     1261 tttttcctta gcagcgcggg cacttacgtc cttgtcatgc aatgttttga tcaataccag
     1321 gattgctacg agagcaatcg cttccctgta actatgccgc catcatgaga ccgcggtccc
     1381 gacgcgtcat gacccgtatt atgtggctcg gcatcgccgg tttatgtgcc gcagtgctca
     1441 gtcttgcagg cgcatttctc tatttggatc cgcagatacc ccgcgcggag acctatcggc
     1501 aggtcaagct cgagactccg ctgcgcgtct actcgagcga cgaaaagcta attgcggaat
     1561 tcggcgagcg gcgggtcata cccctgaagc tggaggaagc gccgccgctg ttcgtcgccg
     1621 cccttctgga caccgaagac aagcgttttt acgaccacag cggcatcgac tacatctcgc
     1681 tgctcaacga caccgtggag ctcgcgctga accgggagat ccgatcgggc gcaagcacca
     1741 tcaccatgca gctcgcgcgc aacatctcct tctccctgga gcagacgttc atccgcaaat
     1801 tcaaagagat gctgctcgcg ctgcagatcg agcaggagct cggcaaggac gagatcctcg
     1861 agctgtatat gaacgtggtg ccgttcggca agcgggccta cggcgcgcag gcggccgccc
     1921 tgacctacta cggcaagcgt ctggacgagc tgaatctcgc ccagctcgcc atgctggcag
     1981 gcattcccca ggccccaacg gcgggtaatc ccatcaacgg gccggcgcgc gcggtgcgcc
     2041 gtcgcaacgt ggtgctgcgg cgtatgctcg atcagggctc gataagtcag gcagctttcg
     2101 acgaagcaat cgccgccccg atcacggcca gcctccacga tcgcgccctg gacgtgggcg
     2161 cgccgttccc ggcggagtgg gtccgcgcgg agctgatcaa tcgctacagc gacctatata
     2221 cgggcgggta ccaggtctac accaccatcg tggccgatct gcagcagacg gccacccgtt
     2281 ccctgcgcgc tggcctgaag agctacgaca ggcgccacgg ctaccgaggc cctgagcagt
     2341 cactgggcgc gctcaccgaa gaggaatacc tccaagcgct gcgcgaggcg agatcctacg
     2401 ccgacctcga tccggccgtc atcgtctccg tggacgagca gggcgcggac gccctgctcg
     2461 gcgacggcag ccgcggccgc atcgccatgg ccgagatgtc atgggcgcga cgctacctgg
     2521 acgtggatcg ccgcggcccc ccgcccgagc ggccctcgga cgtggtccag gtgggcgacg
     2581 tggtgcgggt ggaagctatt gccgaaccgg ccgaggctgg agaggcggaa agtgccggag
     2641 acgatcccga agccgaaccc gcgccggctt ggcggctggc ccagctgcct gacattcagg
     2701 gcgccctggt gtccatggac ccccgtaccg gcgctgtttt agctctggaa ggcggcctgg
     2761 acttcgcgat gaatcagtac aatcacgcgc ttcaggctgc ccggcagccg ggctccggat
     2821 tcaagccctt cgtctactcg gccgcgctcg accgcggggt cacgccggcg agcatattca
     2881 tggacgcgcc cttagtgttc gacgatcaga atctggagtc gcagtaccgg cccgacaacg
     2941 acaacaatcg ctacaacggc ccgaccaggc tccgggaagc cctctaccga tccatcaacc
     3001 tggtctcaat gcgcgtgctg ctcgaggtcg gcgccggcag cgtcatggat cacgtgcgca
     3061 aattcggctt cgacacgagc acgtttccgc gcaaccttca gcttgccatc ggcggcggaa
     3121 ccatggcggt cacgccgctg cagatggcca cggcctatgc tgtctttgcc aacggtggct
     3181 ggcagatcga gccgcacatc gtcgatcggg tggtctcgct ggatggtgaa accgtctacc
     3241 aggccagcca cccgagggtt tgcgatccct gcgatcagga tcccgctacg gacctcgaca
     3301 gcgaggagcc cgcgaacctc gaggatcttc tcgccgaggc cgacgatgcg ccaattgagg
     3361 ccgagcgggt agtcgacgag cggaatacct acatcatgaa cgacatgctc aaggacgtga
     3421 tccgggtcgg gaccggtcgc cgggcgcgta ctctcgagcg aagcgacatc gcaggcaaga
     3481 ccggcaccac caacgacgcc gccgacacct ggttcaacgg ttacagcccc gacgtcgtga
     3541 cgaccgtgtg ggtgggtttc cccgaccacc ggccgctggg cgcaacggag tacggatcca
     3601 acacgcccct gccgatctgg atcgagttca tgtcccaggc actggccaac aagccggaac
     3661 gggttcccgc tcagccggcc ggggtcgtca cgatgaaaat cgacagggac agcggcgagc
     3721 tcgccggctc ccagcagacg gatgcgatat tcgagctgtt tcttgcggag cacacgcccc
     3781 agccaccgcc atcggcggct gccggaccca gcgaacccgg cactgacctc aagccgatgg
     3841 acatcttctg agccgagcct gcagggctac tgaacgatct tgatgtagtc gccggccctt
     3901 ggctccccga tggggtggtc gccgttcaac acgcgcaggg tttctgcggg ataggattta
     3961 atggacacct tgcgggcgag cttgtcgtag ctgtcacccg ggttggcgac cacgacctgc
     4021 agacgctggc tgttggccac catcagatct gccgcggtca tcgcgcgaaa cgactccagc
     4081 gtcgcccgcc attggggctc gaaggcgttg acgtcgccaa ccgggcccac ctctcccttg
     4141 aagagatata ccccgttgtc cttgtagatc accgcgatgc gtctcgcctg cgcgttgccc
     4201 gaggccacct ccacggtgcc gatgaaggcc gagtagccgc tgatctcgag gctctcgccg
     4261 tcggtgacgt cgtcccgttt gagggtttct ttgacgtact cctccggggt ctgctcggat
     4321 tcggcagggt tcaggcgctg gacggtgatg aaggcatcag gcgtcccgcc gggagctctg
     4381 ccgagaactt ccgaggccgt gttcgtgacg tcccatccct gcgggaactg aacgacaacc
     4441 cgcaacccgc cgtgatagta ggtctcgccc tttatcaacc ccgtcgccgc ctcgtccccg
     4501 tagaccatgc cgtcgatcat ctcccagaaa tctctctccg gctcgcgcag ctcgtcggga
     4561 aagatgtgct gagacttctg cacggcgtcg tgcagccgct tgtcgttctt cgggtgcgtc
     4621 gagaacaaac cgtgatagac ggccggctgg ccgagcacgt tcttggcgaa cagcgagtgg
     4681 tccttgagga cgtggatcac gtcgatcatg gctaacgggt tgtaaccggc acgggccaga
     4741 aactctcctc cgtactcgtc agcttcgagc tcgaactcgc ggccatagcc ggagcgcagc
     4801 gtctgggtca ccgtgttgcc catgtctgcg atggcaccgg ttccggtcat gatcgatccg
     4861 atgaacccgg caacgtttcc gaagaaaccc atcatgttgc tgcggcgggc gtggcggccg
     4921 acgacgtggc cgatctcgtg cccgatgacc ccggccagct cgtcctcaga cctgaggtag
     4981 gcgatgagcc cgcggttcac gaatatgtac gcgtcgggaa aagccgaggc gttcaccgtg
     5041 ggattatcga ggatatagaa gtggtaatcc ttgcccgcgt cagggctcac cgcaagcagg
     5101 cgccggccaa tctggttcac gtaggcctgc cactcttggt cctggtagat ctgatcgttg
     5161 gccagcatct ccttgtagac cttctctccc gggccagcca ccgccatgcc ggagaggacc
     5221 aagccgggca gaagcagcgc gcagagctgc aggcgcagct gcgggcgccg tcgcgcgatc
     5281 agccgaccca cctgcgccag cgattgccgc acaggcgccg ggtagccgct tcggagtaga
     5341 cgtcttctca ttcgccggga tccaggaatc acgagcaggt cagccgtatc ttacgagacg
     5401 ctgcgaaaac cgccagggac accgaacctt agactggagg agcatgaaac ggttcaacgc
     5461 cgtccgcgat ccacggggcg gcgtcagcgc tgaggggatg cggtgagggg tgcggtcagg
     5521 aagaaacgga gcggttgccg aagcgcttgc ggaatcgctc gacgcgaccg ccggcgtcca
     5581 ggatcttctg cttgccggtg tagaacggat ggcaagccga gcagacgtcc aggtggatgt
     5641 caccttccag cgtcgagcag gtctctatga cgtttccaca gctgcaagtg gctttgattt
     5701 ggttgtattc gggatggata tccgctttca cctgactgct cccttggatc acgttccggg
     5761 aaaagggcgc gaagtttatt aagaggcgcg gtgaatggca acccgcgcgc cggtcgaagc
     5821 attacaatgc cagctcccga ggagagggct gtgaacccgt tgacccagac gaacgtggac
     5881 gtggtgcgcg tggccgtggc aaagcctctt caccagctgt tcgactacgc ggtgccgccg
     5941 gacatgacgc caccggttgc gggggcgcgt gtcgtcgtgc cgttcggcag gcagcgtctt
     6001 accgcgatct gcgtggagcg caaccccgac gacgcccacg cccagctcaa gcctctgctc
     6061 agcgtcctgg acgataccgc cgccattccc gaggagctgc gcgccctcgc cgggtggctt
     6121 tgcgactact accagcaccc tctcggcgag gtgctggcca ccatgctgcc ggcagaggct
     6181 cgccgcgggc acgagctgac gatcgtcagg ccggagcaat ggacggcgac cggcaccagg
     6241 tccgacctgg cgcgagcgcc ccgccagcag gcgctcatgg aagccctcgc tgccgcgggc
     6301 ggcaccctgt ccgcgtcggc gctcgccgaa gcgggttttt cccgggccct catccgcgct
     6361 gccgcaggca aggggctgat cacccgaacc gaggccgcca cgggctttac cctgggcatc
     6421 gacgagcagc cgccgcagct gagcgacgag caggccggcg tgctctccga cctgctcgct
     6481 cgcaccgatt gctttgcgcc atcacttctg gaggggataa cgggcagcgg caagaccgaa
     6541 atctacctgc aactgatcga taacgtgcgg aaagctggcc accaggttct cgtgctcgtc
     6601 cccgagatcg cgctcacccc tcagaccgtc gggcgcttcg aacgccgctt cggatccggc
     6661 ggcggggtgg ccagcctcca ttccaacctg ggtgatcagg agaggttaca gacctggttg
     6721 aagtgccgca gcggcgaggt cagcgtgctc atcggcacgc gctccgccgt gttcgcgccg
     6781 ttcgcgaagc tgggcctgat cgtggtggac gaagagcacg acggctcctt caagcagcag
     6841 gacgggctga ggtactcggc tcgtgacgtg gcggtgaaac gtgcgcagac attggacata
     6901 ccgctgctgc tcgggagcgc cacgccttca ctggaaagcc tcaacaacgc catggccggc
     6961 cgctacgccc accttcggct gcgccaccgg gcggggggcg cccggccgcc ccggttccac
     7021 ctgctcgaca tccgcggtca gtccctcgat gacggcctca gccaggatct gctgcgcatc
     7081 gtgggccagc atctcaaccg gggcgggcag gccctggtct ttctgaaccg acgcgggtac
     7141 gcgcccacct acctgtgtcc ccgctgcagc tggcaggctg cctgcgggca ctgcgagatg
     7201 cgactgacgc tgcacctgtc gccgcgggcg ctgctctgcc accactgcgg ccatcagcga
     7261 ccgctgccga gcgcctgccc ggactgcggg cacgcggagc tgattgccgt cggtgccggt
     7321 actcagcgca ccgaggccgg gctggctaag cggttcccag ggattcctct gtatcgtgtc
     7381 gaccgcgata ccacccgaag ccagcgtcgc atggaggccc agttcgaggc catccacaag
     7441 ggcgatccgg caatcctcgt cggcacgcag atgctggcca aagggcacca ctttccgaag
     7501 gttaccgtgg tggccgtggt caacgccgac ggcgggttcc tcagcgccga ctaccgcgca
     7561 cccgagcgca cggcccagct catcacccag gtcgccgggc gcgcgggtcg tgccgaggat
     7621 cccggcgagg tgtggatcca gacctatcag ccggacggcg tgctgctgcg ctcgctcatc
     7681 gatgagggct acgacgggtt cgcccggcgg gagctggcga ttcgcaagtc cagcggcctg
     7741 ccgcccttcc ggcccatggc gttgttgcgg gcggagagcc gggacgccga gagcgcccga
     7801 aattttttga ccagcgtccg cgaccgccag ggaagcccct tagaagtgct cggcccggct
     7861 ccagcgccca tcgcccgagt agccaatcgc ctccggtttc agctcatggt gatggcagac
     7921 tcgcgccgcg agctgcatgc tggcatccgg ccgatacgag acctcaccgc gccaggcacc
     7981 atccgctggt ctgtggacat cgatccctac gatacgttct gacctttacc ggccgcccca
     8041 acgggcggtc accggacatc gtggcggacg acccgccgcg agcctatcat tcgcatccca
     8101 tggccaacga ttttgcaaac cgactcaatc cgccgacgac ccggcgcccc aaggcccggc
     8161 cgccctccgc gacacgtcgc aaggagaggc gtttcagcgg ttcgagcttc gcttccggtc
     8221 tgctgatcgg cggcgcgtgc atggccgtgg ctttgaacct ccctgacccg cagcccgaac
     8281 gcccgcccgc cgcagctacc gcggcaagcg caccgcccgg atccgtgacc ttcgagttcg
     8341 ataaggattt gcgcgacgcc tttgtggagg cggaccctgg gcagtacgag acggacgccg
     8401 gccacgacgg atcccgaccg gtcagctatc tcatccaggc ggcgtcgttt ccggtagccg
     8461 ccgatgccga ctcccttcag gcagagcttg cgcaaatggg cctggcggcc cagtcggttc
     8521 cggcgcggct taacgccggc acctggttcc gggtcaccgt cggcccgttc gacagccagc
     8581 tgaaggccaa tcgagcgctt acgcagcttc gcgaaatcga tctggacgcg ttcatgatcc
     8641 agcagcgcct gcccgaaccc cggtgaccaa accccgttgg cggaagtccg ttggcggaac
     8701 cccgatggcg gaaccccgtt gaaatgccgg gcagagggcc ccactatgac cagttcgcaa
     8761 tcggttgaag agctaaatgg cggatatcca cgggacgacc atcgtcagcg tgcgcaacga
     8821 caggcaggtg gccattggcg gagacggtca ggtctccatg ggccagacca tcatgaaggg
     8881 caatgcccgc aaggtgcggc gcctgcatga cgacaaggtc ctggctggtt tcgccggcgg
     8941 aaccgccgac gccttcaccc ttttcgagcg cttcgagggc gtcctcgaca agtatcacgg
     9001 caacctcacc cgtgccgccg tggagctggc caaggactgg cggtcggacc gcgcccttcg
     9061 acgtctcgag gcccttctgg tggtggcgga ccgggaggcc acgctcctgg tgagcgggac
     9121 cggcgacgtc atcgagccgg aagccggcgt gatcgccatc ggctccggcg gcccctttgc
     9181 ccaggcggca gcgcgggccc tgaacgagaa cacggaactg gatcccgccg acatcgtcga
     9241 gaaatcgctg tccatcgccg cggacatctg cgtctacacc aaccatcacc ggaccatcga
     9301 ggtgctggat ctgacatgac ccagatgacg ccgagagaga tcgttcacga gctcgataag
     9361 cacatcatcg gccaggacga tgccaaacgg gccgtggcca tcgcgctgag aaaccgctgg
     9421 cggcggatgc agctcgaaga ggagatgcgc gaggagatat cgcccaagaa catcctgatg
     9481 atcgggccca ccggggtggg caaaaccgag attgcgcggc ggctcgcccg cctggcgaat
     9541 gccccgttca tcaaggtgga agccaccaag ttcaccgagg tgggatacgt gggtcgggat
     9601 gtcgagtcga tcatccggga tctggcagac gtggcggtga agatgctgcg cgaggagcag
     9661 atggaccgcg tcatgcacca ggccgaggat cgggccgaag agcgcatcct ggacgcgctg
     9721 ctccccggac cgaagcccac cggctctcag accatcggct tcacccagga atcctcggag
     9781 gacgaagggg actccaccac gcgtcagctc ttccgcaagc gcctgcgaca gggcgagctc
     9841 gacgaaaagg aaatcgagat cgagacggat gcggcccagg tcggcgtgga gatcatggcg
     9901 cctcccggca tggaggagat gaccagccag ctgcagagca tgttcagcaa cctcggcggc
     9961 ggcagaaaac gctccgtgcg cctgaaaatc aaggacgcgt tgcgcaagct caaggaagag
    10021 gaagcggcga agctggtaaa cgaggacgag atcaaggcac gcgccgtgga aaccgtggag
    10081 cagatgggca tcgtgttcct ggacgagctg gacaaggtgg caaagcgcag cgagagcgcg
    10141 ggcgcggacg tctcccggga gggcgtgcag cgcgacctgc tccccctgat cgagggctgc
    10201 acggtgtcca ccaagtacgg catggtgaaa accgatcaca tcctcttcat cgccagcggc
    10261 gcgtttcacc tggcgaaacc ttcggatctg atccccgagc tgcagggccg gctgcccatc
    10321 cgcgtagagc ttgcggccct gtctgccggc gacttcaagc gaatcctggt cgagcccaac
    10381 gcatcgctca ccgagcagta tcaggcgctg ctggggatcg atgggctcag cctcgaattc
    10441 accgacgatg gcatcgagcg catcgccgag ctggcgtggc aggtcaacga gagcaccgag
    10501 aacatcggcg cgcggcgcct gcacacggtc ctggagcgcc tcctggaaaa cgtttcgttc
    10561 acggctaccg agagcgcgga aagcacggtg gtcatcgacg cggtctacgt ggacgctcag
    10621 ctctcggagc tggcccagga ccgcgatctt tccagataca tcctgtaggc gacgggccaa
    10681 ggccatgcag cgcccaaagc agattaccta tcgccgccag tcaaaagtcc tggttctctc
    10741 tttcgaggac ggcgacgcgg agatccccgc ggagctgctg cgcgtctact caccctctgc
    10801 ggaggtgcgg ggccactcgg ccgcagagcg caagctgcag acgggcaaga agcacgtcgg
    10861 catcaaaagc ctggagcccg taggtaatta cgcgatccgc attaccttcg atgacggcca
    10921 cgacaccggt ctgtacggtt gggaccttct gcgggatctc gccgagaacg ccgacggtta
    10981 ctggaagtcc tatctcgacg agctgaaggc ggccaacgcc tcgcggcttc ccaccatacc
    11041 ggtgggccac tggacgccgg caagccagcc ggccgtcccc ccggagcagg atcaatgagc
    11101 gatcagaact ccgtcgactt cggctcccgc aaggtatcgc cagatgagaa gacccgcctg
    11161 gtaggcggag ttttcgcgag cgtggctgaa cgctacgacg tcatgaacga cctgatctct
    11221 ctgggcaccc accgcctgtt caagcggatg acggtggagc tctcgggagt ccgggagggc
    11281 atgcgcgtgc tggacctggc cggcggcacc ggcgatctga gcgcgttgtt ctctggtgcg
    11341 gttggcccgt cgggagttgt cgtgctcgcg gacatcaacg cacgcatgat ggacgtgggc
    11401 cgcaaccgtc tgctggacca gggttgcgca aacgtctcgt tctgccaggc agcggcggaa
    11461 gctctgccct tcgccgacga cagcttcgac tgcgtgagca tcgggttcgg attgcgtaac
    11521 ttcaccgaca aacgggttgc gctcgaggag ctcaatcgcg tgatcgcgcc gggcggcgtg
    11581 ctgctgatcc tggagttctc caggccggag aacgcatttc tggagggggc ctacgggacg
    11641 tttcagtcgc tctggcccgg actcggcaag ctggtggctg gcgacgcccg aagctaccag
    11701 tacctcatcg agtcgattcg caatcatccc ccgcagaagg cgctcaagct catgctcgaa
    11761 gacgccggct tcgccgccgt cgagtatcac aatctgctgg ggggcatcgc ggccattcac
    11821 cgcggggtca agccatgagc accgcagacg ttttcgaatc agccctggtg gagctcgtca
    11881 acagggcggc caacgcaacg ctcgagctgg accccaaagc cgcatcccgg ctggccgcgg
    11941 cggaaggaca ccgggcgcgc ctcatcgcag cgctgcccgg cggccgtgag caatccttca
    12001 cgctgtcggt cgtcgggggg cgcatggcgt tcacgccgca cgccgaggcc gagcccaacg
    12061 tagtcgtgcg cggttccgtt ccggctctgc tttccatgct cgccggaggc agaagcgccg
    12121 ggccggtcac catcgaaggc gacgtcagcg tgctggagga gctggcggcc atcttcaagg
    12181 gcttcgcacc cgacctggcc gagccgctgt cgaagctggt tggagaaacc tcagcagacg
    12241 gcattctgaa cgcggcggag gcaggtctgg ccctgctgaa atctgcagca gaggctacag
    12301 gcgaatccct gcggcgcggc gtgggcgagc gttttgcgac gcgcacccgc gtcaacgttc
    12361 ttctcgatcg gctggaagat gcccgcgaac gatcggaccg cctcgccgca cgcataggca
    12421 atctggagag cgcccggggc tcccaccagc gcccggaacc tccaacgcca tgagcgctct
    12481 gcgccgactc tttcgggttc tctggatcgt gagccgctac cggctcgaca ccctgatcga
    12541 cgcggatcag gaaggcgcgg cgttgccgcg tcgctacgcc tggctactgc gcgcttctcc
    12601 cgcccggctt ctaccggtcg gcgacgaacc gcgcgggcgg cgcctgctgc tggcgctgga
    12661 gcagctcggg ccggtgttcg tcaaattcgg acagctgctt tccacgcggc gggatctgat
    12721 accgctggac attgcggacg agctcagcgc cctgcaggat cgggtgcccc cgtttcccgg
    12781 cgagcaggcc gtgcggatca tcgaggacgc gctgggccaa cccatcagcg aagccttccg
    12841 ctccttcgag atagagccca tggcatccgc gtctgtggcc caggtgcatg ccgccatcct
    12901 gccggacggc aagagcgtgg tggtcaaggt cgtcagaccc gacatagccg acgtcatccg
    12961 ggcagatctg gaactgctgc gcgccgccgc ggagattctg gagaacgcct ctgaggaggc
    13021 gcggcggctg cacctcaagc aggtggtcaa ggactacgag caaacgattc tcggtgagct
    13081 cgacatgcag aaggaggcgg acaacaccgc ccggctgcgc agcaacttcg ccgagtcgga
    13141 gctcctgtac gtgccgcgcg tctactggcg ctggtgccgc agcaacatcc tggtgatcga
    13201 gcgtatccac ggaataccga tctccgatgt ggccgagctc aaagcccgcg ggacgaacat
    13261 gcggcgcctg gccgagaaag gggtggagac ctttttcaag caggtgttcg tggacaactt
    13321 cttccacgcg gacatgcacc cgggcaacat cttcgtcgac gtgaccgatc ccgaggaccc
    13381 gcgctacatt gccgtggact gcgcgatcat cggctcgctc aaccaggagg atcaggacta
    13441 cctcgcccga aatcttctgg cgttcttcaa ccgggattac gccgaggtcg cgcggctgca
    13501 tctggagtcc ggctgggttc ccgacgaaac ggaccccgaa gagttcgagc gggtcattcg
    13561 cgaggtctgc gagcccatct tcgcaaaacc gctcaacgag atctctttcg gtcagtttct
    13621 cgtggagctc ttcgaaacgg ccagcgcctt tcacatggag attcagcctc agctggtgct
    13681 gctgcagaag acgcttctgt acatcgaagg cctgggtcgg gagctgtacc cggagctgga
    13741 tctctgggag acggccaaac cgttcatgga atcctggatg gccgagcggg tgggaccggc
    13801 ggccgccctg cgggagctgg cagaacgcgc gccggagatt ctcgcggagc tgccgcgcct
    13861 gcccggcatg gtggtgacgg ccagccggca gatacgtcac ctcgaaagga tggtggaccg
    13921 acagaaccgg gaactgcacg agctcgaggc gcgcgtgggc aggctcgccc ggggcgtgcg
    13981 cggacgccgg gtggcgggcg ccgctttgct cgtgctggcc agcgtcctac tatggggccc
    14041 tatcgctgaa tcgctgagcg gcagcggaga tctcggcacc gtggctggtc tcgccagcgc
    14101 ggccatcgct tcgttgctgc tcttgaaggc ctgaaacatg accgatagca aagacatcgt
    14161 cgaagacctg atcgaagttc tcgaatcccg ccgcagcgcc gaccccggtt cgtcctacgt
    14221 cgcatcgctg tacgctgacg gcctgaacag gattctcgag aaagtgggcg aggaagccac
    14281 cgaggtggtg ctcgctgcca aggatgcgac gtcgtccgac agggacgatg cgctcgttca
    14341 cgaggtggcc gatctgtggt ttcacactct ggtcatgctc gtgcatctca atcaggaccc
    14401 acgctgggtt ttcgaaacgt tgcgagagcg tttcggcatc tccgggcacg aggagaaaag
    14461 cagccgcaac gccggcggcc cctgacgggc ctattggatt ccgacgaggt gggctgggtt
    14521 cggaagacgt atacttgacc aacgacctga cggagacagc aaatgtttgg actgggcatg
    14581 accgaactca tcgtcatcct catcatcgtg gtcctcatat tcggccccaa gcggctgaaa
    14641 accatcggct ctgacctggg aaacgccatc aaggggtttc gcaaagccgt gaccgaggaa
    14701 gaggccaagg ccgcgcccgc ggaggagccc cgcgtgatcg aaggtgaggt caacgcggcg
    14761 cagaagactg agaccaagca ggactccagc gtctagcaat gttcgacatc ggctttccgg
    14821 agctcgttct catcgccgtg gtcgcgctgc tggtcatcgg accggagcgg ctgcccgaag
    14881 cgctgcggac cctaggactg tggctcggca gaatgcgccg cagcttcacc gctgtgaaga
    14941 cggagatcga gaaggagatc ggcatggacg acgtgcgccg ccaactccac aacgaggcca
    15001 tcatggagga gatgaagcgc atcgagggcg aggtgcgtgg aaccgccgac agcccggccg
    15061 gcgatgctca gaccgaggct gccggaaacc cgccttcaaa cgaaacctcg gccactgcgg
    15121 acaatggcgg gcgctgaaga ttccgacaac cggctcagcc acgacgagca ggtagacgcc
    15181 gacgagcagc cgtttctcga tcacgtcatc gagctcagat cgcgcatcct gcgcggcctt
    15241 ctggtggtcc tgctgctctt cttccctacc tactactacg caaacgacct gtacgagctg
    15301 gtcgcagctc cgctgttggc gcacctgccc ggaggcggca agatgatcgc taccgaggtg
    15361 gcgtcgccat tcctcacgcc cttcaagctg tcggtcttca ccgccgtgtt catcggcatg
    15421 ccgtttctgc tgcaccagat gtgggcgttc atctcgccgg ggctgtacct gcacgagaag
    15481 aagttcgccg tccccgtgct gctgtccagc atggtgcttt tctacggcgg cgtcgcgttc
    15541 gcgtattttc tggtctttcc cctggtcttt cagttcatgg ccgctgtcac gccggagggc
    15601 gtgaccatga tgaccgacat caatcgatat ctg
//
LOCUS       DBNHKV010000135         2581 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1152918, whole genome shotgun sequence.
ACCESSION   DBNHKV010000135 DBNHKV010000000
VERSION     DBNHKV010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1152918"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1835
                     /gene="xrtE"
                     /locus_tag="AC16PP_04465"
     CDS             192..1835
                     /gene="xrtE"
                     /locus_tag="AC16PP_04465"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase E/protease, VPEID-CTERM system"
                     /protein_id="HIR6480911.1"
                     /translation="MSSRADLLRHAPLRLDARARLAVFALLLTGEALFVSLTYDAWGS
                     AAAGAGWWLIALNYAGYALKALIAFIVAMLLAVGPRLKAYHLALSPISVRGLLLRMLP
                     QLGAFGAFLVATDGIFGAESGLFPAWLPAAWLALAVLVAVLALRLVARIGFWTGFARQ
                     ETGAMIMAGAVAIASLTIAMASQQLWEPLSDLTFAVSAQLLGLMYSDIYADPLLRILG
                     TDTFRVSIDTACSGYEGIGLVVVFTAFVLSLFRADFRFPQALLLFPIGIAAIWSFNAI
                     RIALLIGIGHELSPEIAVGGFHSQAGWISFVLVAVGLLAGAYRISFFRADTPVPTRSA
                     VVTAGLNANVAYLLPIVALLGSTLLTLAFTADMDWLYPLRVVMVGAVLALAWRHFRFV
                     WAPVSWVAVGGGFLVLALWLALVPTDPAQDALVGAKIGEAPLWIAALWIIFRCVGSAV
                     TVPLAEELAFRGYLMARLTPGEPSPADRLPFSWLALLVSSAVFGLLHGAWLAGAIAGL
                     TYGLIRYHRGRVADAVVAHAVTNAMLSVYVLVTGHWSLW"
     gene            complement(1889..>2581)
                     /gene="hpaH"
                     /locus_tag="AC16PP_04470"
     CDS             complement(1889..>2581)
                     /gene="hpaH"
                     /locus_tag="AC16PP_04470"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009049320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HIR6480912.1"
                     /translation="DAYAVQDAWVKLKIDSGRRIVGRKVGLTSRAMQEAMRIAEPDFG
                     TLLDDMMFEDGASLKAGDFLDPRIEVELAFVLGQTLAGADVTLDDVLEATDYVTPALE
                     IIAARSFRVDPQTGSPRTVVDTISDNAANAGVIVGGRRVRPDELDLRWVSALLYRNGI
                     VEETGVAAGVLDHPGNGVVWLARKFAEHGVSLERGEIVLAGSFTRPVAVRPGDEFRAD
                     YGPLGVIGCAFS"
BASE COUNT          367 a          938 c          788 g          488 t
ORIGIN      
        1 gccgcgcgag ggcaggcggc ttacatcagc ttcgctcgag cggcctcagc gcgggccccg
       61 aatgtaagcg aaccgggaac accgtaggct gcttccctac attcgggcca caacggcacc
      121 acacgctaca ttccgcgtgc gcctatcgtt aagcgcatgc gtgttacacc cgtatccgta
      181 ggcatccgag catgagcagc cgtgccgacc tgctgcgcca tgcgccattg cgcctcgacg
      241 cccgggcccg tctggccgtc ttcgcccttc tgctcaccgg cgaagcgctg ttcgtcagcc
      301 tcacctacga cgcctgggga agcgccgccg caggcgcggg ctggtggctc atcgccctaa
      361 actacgccgg ttacgcgctc aaggcgctga ttgccttcat agtcgccatg ctgctggcgg
      421 tgggtccccg actgaaggcc taccacctgg cgctgtcccc tatctccgta cggggcctcc
      481 tcctgcgaat gctgccgcag ctgggggcct tcggcgcttt cctcgtggct acggatggga
      541 ttttcggggc cgaatccggt ctcttccccg catggctacc cgccgcgtgg cttgcgcttg
      601 cggtgctggt cgcggtgttg gcgttgcgat tggtcgcccg tatcgggttc tggacgggct
      661 ttgcacgaca ggaaaccggc gccatgatca tggccggcgc ggtcgccatt gcaagcctga
      721 ccatcgccat ggcctcccag cagctgtggg agccgctcag cgacctcacg tttgccgtaa
      781 gcgctcagct gctcggcctg atgtattccg acatctacgc agatcccctc ctgcgcatac
      841 tcggcaccga taccttccgc gtttccatcg acaccgcctg ctcaggctac gaaggcatcg
      901 ggctggtcgt ggtgttcacg gccttcgtcc tatccctgtt cagggccgac ttccgcttcc
      961 cccaggcgct gctgctgttt cccatcggca tcgccgcgat ctggagcttc aacgccatcc
     1021 gcatcgcgct gctcatcggc atcggccacg agctctcacc cgagatcgcg gtgggaggct
     1081 tccacagcca ggccggctgg ataagcttcg tcctggtcgc cgtggggctg cttgccggtg
     1141 cctaccgcat ttcgttcttc cgcgccgaca ctcccgtacc cacgcgatcg gccgtggtca
     1201 cggcgggcct gaacgcgaac gtcgcctacc tgctgcccat cgtcgcgctg ttgggatcga
     1261 cgcttctgac gctcgccttc accgccgata tggactggct gtacccgctg cgggtcgtca
     1321 tggtcggcgc cgtcctggca ttggcgtggc gccactttcg attcgtctgg gcgcccgtgt
     1381 cctgggtcgc cgtgggcggg ggatttctcg tattagcgct gtggctggcg ctcgtgccca
     1441 cggatcccgc ccaggacgct ctcgtcggcg caaagatcgg cgaagccccg ctctggatag
     1501 ctgcgctgtg gatcatattc cgctgcgtcg gctcggcggt cacggttccg ctcgcggagg
     1561 agctggcctt tcgcggctat ctgatggccc ggctcacgcc cggagaacca tcccccgccg
     1621 accgcttacc cttctcctgg ctggcgctgc tcgtctcgtc ggccgttttt ggcctgctgc
     1681 acggggcctg gctggccggc gccattgccg ggctcaccta cggtctgatc cgctatcacc
     1741 gcggccgcgt agccgatgcg gtggtcgccc acgcggtcac caacgcaatg ctgtcggtct
     1801 acgtgctcgt caccgggcac tggtcactct ggtagcggcc ggcggtcgag cccccgacaa
     1861 agccacaaca agccacacac cggccctatc aggagaaggc gcagccgatg acgcccagcg
     1921 gcccgtagtc agcccggaac tcgtcgccgg gtcgaacggc caccggccgg gtgaaggacc
     1981 ccgccagaac gatctccccc cgctcgagcg acacgccgtg ctcggcgaac tttcgggcca
     2041 gccagaccac gccgttgccc ggatgatcca gcaccccggc agccacgccg gtctcctcga
     2101 cgattccgtt gcggtacagc aaggcagaca cccagcgcag atcgagctca tcgggcctca
     2161 cacggcgtcc gcccacgatt acgcccgcgt tggccgcgtt gtcagagatg gtatcgacca
     2221 ccgtccgcgg cgaaccggtc tgcgggtcca ctcgaaagct gcgggccgcg atgatctcca
     2281 gcgccggggt cacgtagtcg gtcgcctcga gcacgtcgtc cagcgtgacg tccgcaccag
     2341 ccagggtctg tccgagcacg aaagccagct ccacctcgat gcgcggatcc agaaagtcgc
     2401 ccgccttcag gctcgcgccg tcctcgaaca tcatgtcgtc cagcagcgtg ccgaaatcgg
     2461 gctcagcgat ccgcatcgcc tcctgcatgg cccgggacgt gagcccgact ttgcgcccga
     2521 cgatgcgtct gccggaatcg atcttcagct tgacccacgc gtcctgcacc gcgtaggcgt
     2581 c
//
LOCUS       DBNHKV010000136         2204 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1294184, whole genome shotgun sequence.
ACCESSION   DBNHKV010000136 DBNHKV010000000
VERSION     DBNHKV010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1294184"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="AC16PP_04475"
     CDS             <1..219
                     /locus_tag="AC16PP_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480913.1"
                     /translation="ELKGQCSIVVIAHRLSTVREADRILVLSEGRLQASGRHEELLKS
                     SELYARFSRIQFAGEDEADGSGARTTAG"
     gene            287..1420
                     /locus_tag="AC16PP_04480"
     CDS             287..1420
                     /locus_tag="AC16PP_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6480914.1"
                     /translation="MGPLHGFRIVELAGIGPGPFCGMMLSDMGAEVIRVDRISGGANR
                     PKDVLARNRRSIAVDLKSPAGAETVLRIVEKSDALFEGFRPGVTERLGLGPEDCWARN
                     EKLVYGRMTGWGQEGPLAQAAGHDINYIGLSGALHAIGHAGDRPVPPLNLVGDFGGGG
                     MLLAYGLVCGLLEAQKSGKGQVVDAAMVDGAAALMAMFFSMGAAGVFKDQRGTNLLDS
                     GSHFYDTYETKDGKYVCIGSIEPQFYALLVEKAGLDAERFAAQMDASRWPDYKRELTE
                     VFKSKTRDEWCEIMEGSDVCFAPVLSIFEAPDHPHSQARNAFIELDGVVQPAPAPRFS
                     RTEVAASHGARVPGEDSVSVLEDCGFTGDEIAGLKNDGVIGGN"
     gene            1436..1972
                     /locus_tag="AC16PP_04485"
     CDS             1436..1972
                     /locus_tag="AC16PP_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480915.1"
                     /translation="MAVELLDTERFHGFRVRRQIAGKTYQEYFSLKDDGKRIKGPQRA
                     TVKALAEARDAELANLQKAARERADRTVRVDARGQVRGILCRLKTEKSGTLTPVFQIG
                     VMSRLEDKIVNTTVSISRYGETEAWRRAIDFYALHKGISKRSRAYRDLLAAQPSGAKI
                     KSLLKASGRAAPRRSARR"
     gene            1969..>2204
                     /locus_tag="AC16PP_04490"
     CDS             1969..>2204
                     /locus_tag="AC16PP_04490"
                     /inference="COORDINATES: protein motif:HMM:NF027503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6480916.1"
                     /translation="MSYASEYARSLYDPEGFWREKAAELPWLEFPQTILDRAPSGAWR
                     WFHGGKTNTCWLALDRHVADGRGAQVALNSDSPAT"
BASE COUNT          418 a          675 c          754 g          357 t
ORIGIN      
        1 gaactcaagg gccaatgcag catcgtcgtc atcgcccacc gtctgtccac ggtccgcgag
       61 gcggatcgga tactcgtgct gagcgaggga cggctccagg ccagcgggcg ccacgaggag
      121 cttctcaaaa gcagtgagct gtatgcccgt ttctcgcgta ttcagttcgc cggcgaggac
      181 gaagcggacg gctcgggtgc tagaaccacc gcgggatgat gcccacgcaa aagatcgcta
      241 aactcctcgg ctgatagtct gatttcttac cggggggaag gtgagcatgg ggccgttaca
      301 cgggtttcgt attgttgagc tggctgggat cggtcccgga cctttctgcg ggatgatgct
      361 gtccgacatg ggcgccgagg tgatccgggt ggaccggatc agcggcgggg ccaaccgccc
      421 gaaggacgtg cttgcccgca atcgacgctc catcgccgtg gacctgaaga gcccggcggg
      481 cgcggaaacc gtgctgcgca tcgtcgagaa atcggatgcg ctgttcgagg gtttccgccc
      541 gggcgtgacc gaacggctcg gccttggtcc cgaggattgc tgggcgcgca acgagaagct
      601 cgtttacggg cgcatgacgg gctggggcca ggaagggccg ctggctcagg cggccgggca
      661 cgacatcaac tacatcggtc tgtcaggggc tcttcacgcg atcggtcacg cgggggaccg
      721 cccggtgccg ccgctgaacc tcgtcggcga tttcggaggc ggcgggatgc tgctcgccta
      781 cggcctggtc tgcggcctgc tcgaagccca gaaatccggc aaggggcagg tggtagatgc
      841 ggccatggtg gacggggcgg cggccctgat ggccatgttc ttctccatgg gcgctgccgg
      901 ggtgttcaag gaccagcgcg gcaccaacct gctcgacagc ggctcgcact tctacgacac
      961 ctacgagacg aaggacggga agtacgtctg catcggctcc atcgagcccc agttctacgc
     1021 cctgctggtc gagaaagcag gcctggacgc ggaacgcttt gccgcccaga tggacgcctc
     1081 cagatggccg gactacaagc gtgagctgac cgaggtgttc aaaagcaaga cgcgcgacga
     1141 gtggtgcgaa atcatggaag gcagcgatgt ctgcttcgct cccgtgctgt ccattttcga
     1201 agcccccgac catcctcaca gccaggcccg caacgccttc atcgagctgg acggcgtcgt
     1261 ccagcccgcg ccggcccctc gcttctcccg aaccgaggtg gcggccagcc acggtgcccg
     1321 ggtacccggc gaggactcgg tatcggtgct ggaggactgc ggatttacag gagacgagat
     1381 cgccgggctc aagaatgacg gcgtgatcgg cggcaactga ccggaggatc ggcagatggc
     1441 ggtagagttg ctggacacgg agcgttttca cggattccgt gtgcgcaggc agatcgctgg
     1501 gaagacctat caggagtatt tctctctcaa ggacgacggc aagcgcatca aaggtccgca
     1561 gcgggccacg gtcaaagccc tggccgaagc ccgcgatgcc gaactcgcga atctgcagaa
     1621 agcggcgcgg gaaagagcgg acaggacggt gcgcgtagat gccagaggcc aggttcgcgg
     1681 aatcctgtgt cggctcaaga ccgagaagag cggtaccctg acaccggtat ttcagatcgg
     1741 cgtcatgtcg cgcctggaag acaagatagt caacacgacc gtttcgatca gccgctacgg
     1801 cgagacggag gcctggcgcc gcgccatcga cttctacgcg ctgcacaaag ggatcagcaa
     1861 acgatcccgc gcctatcgtg acctgctggc agcgcaaccg agcggcgcga agatcaagag
     1921 cctgctcaag gcgtccgggc gcgccgcacc gcggcgatcc gcccgaagat gagctacgcg
     1981 tcagagtacg cgcgatcgct ctatgatcca gaagggttct ggcgggaaaa agcggcagag
     2041 cttccctggc tcgagtttcc gcagaccata ctggaccgcg cgccgtcggg cgcctggcgc
     2101 tggtttcacg gcggcaagac gaacacctgc tggctggccc tggacaggca cgtggcggac
     2161 ggccgcgggg ctcaggtcgc gctcaactcc gattcgcccg cgac
//
LOCUS       DBNHKV010000137         9439 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1224214, whole genome shotgun sequence.
ACCESSION   DBNHKV010000137 DBNHKV010000000
VERSION     DBNHKV010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9439
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1224214"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="AC16PP_04495"
     CDS             complement(<1..418)
                     /locus_tag="AC16PP_04495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6480917.1"
                     /translation="MNVQPLSVRERMGYGVGDFGVNLFFIPAMTYLLYFYTDVFGLSA
                     AAAGGVFLVARVVDAVTDPLMGGIAERTSSRWGRLRPHILFGALPLGAIAVLTFTTPD
                     LNDAGKLWWSAVTYVLFGIAYTVVSIPYSALTASITA"
     gene            complement(426..1625)
                     /locus_tag="AC16PP_04500"
     CDS             complement(426..1625)
                     /locus_tag="AC16PP_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6480918.1"
                     /translation="MGPLSHIRVLDLTIARAGPSAVRLLADWGADVIKIEPVPGAGQS
                     VTGARRGPDEQNLHRNKRSLCLDLKHPDGHGLFLELAAVADVVVENFKVDVKHRLRVD
                     YETLKQVNESIIYASISGFGQEGPYADRGGVDQIVQGMSGLMSVTGEPGGGPMRVGVA
                     ISDTSAGMFLGQGILLALLHRERTGEGQWVHTSLLEAMMCKLDFQGARYTMTGEVPTQ
                     QGNDHPAYTPMGTFLASDGQVNIAASMPRLWQRFCEALGADDLLAHPDYQTTADRIAR
                     KDQLKEAINAITSQRTWADLVGVLNEAGVPCGAINTIGEGFEDPQVRHLGMAKPAPHP
                     DMGSVNLIRSPINLSRFPQESAFRNAAPDPGFDTRAVLTELGIPSSRIDALAAAGAIA
                     CTDPASA"
     gene            complement(1627..3324)
                     /locus_tag="AC16PP_04505"
     CDS             complement(1627..3324)
                     /locus_tag="AC16PP_04505"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6480919.1"
                     /translation="MSEHELVIRGGTVVDGTGSPERTADVAVRAGVIVAVGEVDGSGR
                     REIRADGALVTPGFVDIHTHYDGQATWSNRLSPSSHHGVTTVVMGNCGVGFAPVKPTD
                     RDLLIELMEGVEDIPGAALHEGIHWEWETFPQYLDYLSQRSFDMDIGAQLPHGALRVY
                     VMGRRGADREAASADDIARMRALTAEAIEAGALGFSSSRTLNHRSSKGAPTPSLTAAR
                     QELLGIARGLKDAGRGVMEMISDFQDADEEFAHLTAMTRESGRPMSISLAQGLSPNGW
                     RGLLGRIASASEAGLPMRAQVAPRPIGILLGLTTTLNPFVTHPAYREIAGLALAERVR
                     AMRDPERRAAILAGSPGRGFQRLWGLMANGAKIWRLEDPPDYEPAPQDSLASRARVAR
                     VDPWEYVYDQLLQAEGRQILYTPFANYAEDNLDCCREMILHPDTVMGLGDGGAHVGTI
                     CDASFVTTLLTHWGRDRTRGERIPLPVLVKSQTADTARAVDLNDRGVLAPGMKADINV
                     IDFERLKVRAPEIVHDLPAGGARLQQKADGYLATIVSGQVTYENGEATEALPGRLIR"
     gene            3420..5264
                     /locus_tag="AC16PP_04510"
     CDS             3420..5264
                     /locus_tag="AC16PP_04510"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HIR6480920.1"
                     /translation="MQRVTIILIVLSGLILALFAWFPAETLLDAARALDRTPPEPATP
                     DDVTRRTTESGDVVGFIGNHGARTWRGVPYARPPVGALRWRAPEPAEPWPGVREATAA
                     GPLCAQRAGVISGDGFAPTRVAAGSEDCLYLDIYAPANARRLPVMFWIHGGGNSVGHN
                     GPYDGARLATWRDVVVVTVNYRLAHLGWFAHPALTTGNRLHDSGNYGTLDLIQALTWV
                     RDNIQAFGGDPDNVTVFGESAGGTNTLSMLASPRAAGLFHRAIAQSGGLTPMPMARAS
                     AYAEDGGHPSSAREIVNRLLIADGRASSPEDARRVQENMPAGAIRDYLYGKSLTELFA
                     VFDNGFAGMIDTPEALGDGHVLPDGPLADVFGDPSRHNAVPVILGSNRDEPALFMAAS
                     PEHVNTYLWIFRRLKDEAGYRRQVKYLGQAWKARGVDEFANAMAAAGNPHVYAYRFDW
                     DEEPSVLGFDLSVALGAAHALEIPFVFGSFSGGLGLGYLYHESEGRHALAESITSYWT
                     EFAYNGDPGTGRDGGEVRWLPWGRNGATSIVLDTLEDKGIYMTTERVTTDSVKTAIAT
                     DPDVPDQRERCLLYYRAFRWGSQFDVDEFAGFGDGGCAAYDPAALGDF"
     gene            complement(5287..6519)
                     /locus_tag="AC16PP_04515"
     CDS             complement(5287..6519)
                     /locus_tag="AC16PP_04515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6480921.1"
                     /translation="MARGPTTVSTTFQAGWRSPLMLLMLMSVAMPVAFNAWSALLNNF
                     VVERAAFTGVEIGILQSLREVPGFLAFTTIFVLLVVREQTFAVLSLAMLGLGVALTGF
                     LPSEYGLYFTTVVMSTGFHYFETLKQSLSLQWLHRDEAPGMLGRLLAVGSLTSLVTYG
                     VLWVLLELFRLDYVWNFLISGGVCVGLAVVMWLWFPHFETKTPQTKRLVLRRRYWLYY
                     ALTFFSGARRQIFVVFAAFLMVEKFGYSAAQVTLLFLINYAFNWAFAERIGRLIGRFG
                     ERAALTFEYIGLIGVFVGYAFVSDPYVAAGLYVVDHMFFALAIAMSTYFQKVADPADI
                     ASSAGVSFSINHIAAVVVPAALGLLWIHSNALVFLIGAGFAVCSLLLSQLIPERPAPG
                     NEIARGRLFPQPADSAGR"
     gene            6719..7084
                     /locus_tag="AC16PP_04520"
     CDS             6719..7084
                     /locus_tag="AC16PP_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR6480922.1"
                     /translation="MPDWKDNEHIYLQLKDLVLRQIMLGTLLEGEPVPSVRQVASQER
                     INPLTVSRAYQMLVDEGLLEKRRGVGMFVCEGAREQALKQERTRFLEEDWPEVVERIA
                     LLGLTRDELLNTAAEEGRQ"
     gene            7081..7935
                     /locus_tag="AC16PP_04525"
     CDS             7081..7935
                     /locus_tag="AC16PP_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6480923.1"
                     /translation="MSLITARGLAKSFGDVRALDGLDLTVEAGRIVGLIGPNGSGKTT
                     ALKAILGLSHLDAGTLDVLGMNPATSRKALMQRTAYIADVGILPRWMRVSQLLDFVAG
                     VHPGFRREVAEAHLESTDVRPDKRINGLSKGMNVQLHLALVLAIDAELLVLDEPTLGL
                     DIIYRQKFYDALLGDYFDGNRSILVTTHEVREIEHILTDVVFIHHGRSLLSLSMESIH
                     DEFAKVVAQPTQAGAVRSLGPIAERTTLSGAEFVFRGADRDALGSYGEVSTPSLPELF
                     IAIMGDNA"
     gene            7932..8882
                     /locus_tag="AC16PP_04530"
     CDS             7932..8882
                     /locus_tag="AC16PP_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480924.1"
                     /translation="MSTMVALIKREWQEHQVGLGWSPVAILAVLALITFMALATSTVG
                     GVNVSVEGSEGSHSFHEEVEISSLSALAQFMELDRWSDAELEENLAKFRSLVAMPFKA
                     VYFLIAVFVLISALHDDRRDRSVLFWKSMPVSDAETVLSKLVTVVWVAPVVVIAGIVA
                     AQLFLLIVISTLTLVEDVGGAGRLWWHSGLLTGTVELVVGYVIQGFWALPLYAWLLAV
                     SAAAPRLPILWAVLLPGLPAIAERMVLGTHGVSDFVRAHLEFAALPAAASEDDGIIQP
                     AVGLAEQLSLVFSFQMWLGVVVGGLLLWLAVYLRNRNNDL"
     gene            8969..>9439
                     /locus_tag="AC16PP_04535"
     CDS             8969..>9439
                     /locus_tag="AC16PP_04535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6480925.1"
                     /translation="MAWDFSTEPEFQKELDWMDEFVREEIEPLDFIIGHPNDFSDPNR
                     AKLIPPLQEEVKKRGLWACHLGPELGGQGYGQVKLGLMNEILGRAKCAPVVFGCQAPD
                     SGNAEILAHYGTEQQKQRFLQPLLDGEIVSCYSMTEPHAGADPTMFRTRAVRDGD"
BASE COUNT         1584 a         3235 c         3144 g         1476 t
ORIGIN      
        1 cggccgtgat cgacgcggtc agcgccgagt agggaatgga gaccaccgtg taggcgatgc
       61 cgaacagcac gtacgtgacg gccgaccacc agagcttgcc ggcatcgttc aggtccgggg
      121 tggtgaaggt gagcacggcg atcgccccca gaggcagcgc gccgaagaga atgtggggcc
      181 gaagccgccc ccagcggctg ctcgtgcgct cggcaatccc gcccatgagc gggtcggtca
      241 ccgcgtccac cacccgggcc acgaggaaca cgcctccggc ggccgctgcg ctcagcccga
      301 acacgtccgt gtagaagtac agcaggtacg tcatggccgg gatgaagaac agattgacgc
      361 cgaaatcgcc cacgccgtag cccatgcgct cccgaaccga aagcggttgg acgttcacgg
      421 cggcttcagg ccgatgccgg gtcggtgcag gcgatggcgc ccgccgccgc cagcgcgtcg
      481 atgcgggagc tgggaatacc gagctcggtc agcacggcgc gggtgtcaaa accggggtcc
      541 ggagccgcgt tgcggaaggc cgactcctga ggaaagcgcg acaggttgat gggggagcgg
      601 atgaggttca ccgaacccat atcagggtgc ggcgccggct tcgccatgcc caggtggcgc
      661 acctgaggat cctcgaaacc ttcgccaatg gtgttgatgg ccccgcacgg cacgcccgcc
      721 tcgttcagga cgcccaccag atcggcccac gtgcgctggc ttgtgatggc gttgatcgcc
      781 tccttgagct ggtccttgcg cgcgatgcga tccgccgtgg tctggtaatc gggatgggcc
      841 agcagatcgt ccgcccccag cgcctcgcag aaccgctgcc acaggcgcgg catggaggca
      901 gcgatgttca cctggccgtc gctggccagg aacgtgccca tgggggtgta ggccgggtgg
      961 tcgttgccct gctgggtcgg cacctcgccc gtcatggtgt agcgggcgcc ctgaaaatcc
     1021 agcttgcaca tcatggcttc gagcagcgac gtgtgaaccc actgcccctc gcccgtccgc
     1081 tcccgatgca gcagcgccag caggatgccc tgccctagaa acatgccggc ggacgtgtcg
     1141 ctgatggcca cgcccacccg catggggccg cctcccggct cccccgtcac cgacatgagc
     1201 ccgctcatgc cctggacgat ctgatccacg ccgccccggt ccgcgtaagg gccctcctga
     1261 ccgaagcccg agatgctcgc gtagatgatg ctttcgttaa cctgcttgag cgtctcgtag
     1321 tccaccctca ggcggtgctt gacgtccacc ttgaagttct cgaccaccac gtcggccacg
     1381 gctgcgagct cgagaaacag cccgtggcca tccgggtgct tgagatcgag acagaggctg
     1441 cgcttgttgc ggtgcaggtt ctgctcgtcc ggcccgcgcc tggccccggt caccgattgc
     1501 cctgcgcccg gcacaggctc gatcttgatc acgtcggcgc cccagtccgc cagcagccgt
     1561 acggcactcg ggcccgcgcg ggcgatggtg aggtcgagca cgcgaatgtg ggaaagcggc
     1621 cccatctcag cgaatcagcc ggccgggcag cgcctccgtg gcctcgccgt tctcgtaggt
     1681 gacctggccg ctgacgatgg tggccagata accgtccgcc ttctgctgca ggcgggcacc
     1741 gccggcgggg aggtcgtgaa cgatctccgg cgcgcgcacc ttcaggcgct cgaagtcgat
     1801 gacgttgatg tcggccttca tgccgggtgc gaggacaccg cggtcgttga ggtccacggc
     1861 ccgggccgta tccgccgtct ggcttttcac cagcacgggt agagggatgc gctcgccccg
     1921 ggtgcggtcc cggccccagt gcgtgagcag ggttgtcacg aagctggcat cgcagatcgt
     1981 gcccacgtgg gcgccgccgt cacccaggcc catgacggta tccgggtgca ggatcatctc
     2041 ccggcagcag tccaggttgt cctcggcgta gttggcgaac ggcgtgtaga gaatctgccg
     2101 cccttcggct tgcagaagct ggtcgtagac gtactcccag gggtccaccc gggccacccg
     2161 ggcgcggctc gccaggctgt cctgcggcgc cggctcgtag tcgggcgggt cctctaaacg
     2221 ccagatcttg gcgccgttgg ccatgagccc ccacaggcgc tggaatccgc gccccgggga
     2281 tcccgcgagg atggcggccc tgcgttccgg gtcgcgcatg gcgcgcaccc gctcggccag
     2341 cgccaggccg gcgatctccc ggtaggccgg gtgggtgacg aacgggttca gcgtggtggt
     2401 gagccccagg agaatgccga tgggccgggg ggcgacctgc gcgcgcatgg gcaggcctgc
     2461 ttcgctggcg ctggcaatgc ggccgagcag gccgcgccag ccgttggggc tcaagccctg
     2521 agccagcgag atggacatgg gtcggccgct ctcccgcgtc atcgccgtca gatgagcgaa
     2581 ctcctcgtcg gcatcctgga agtcggaaat catctccatg acgccgcggc ccgcgtcctt
     2641 gagcccgcgg gcgatgccga gcagttcctg cctggccgcg gtgagcgaag gcgtcggtgc
     2701 gcccttgctg ctgcggtggt tgagcgtgcg cgagctggaa aaacccagcg cgccggcctc
     2761 gatggcttcc gccgtgagcg cgcgcatccg ggcgatgtcg tctgcgctgg ctgcctcccg
     2821 gtccgcgcct cgccgaccca tgacgtacac ccgcaaggcg ccgtggggca gctgtgcgcc
     2881 gatgtccatg tcgaacgagc gctgcgatag atagtcgagg tactggggaa aggtctccca
     2941 ctcccagtgg atgccttcgt gcagcgcagc gccagggatg tcctccacgc cttccatgag
     3001 ctcgatgagc aggtcccggt cggtgggttt gacgggcgcg aaccccacgc cgcagttgcc
     3061 catcaccacc gtggtgaccc cgtgatggct cgagggggac aggcggttgg accaagtggc
     3121 ctggccgtcg tagtgagtgt gaatgtccac gaagccgggc gtgaccaggg cgccgtcggc
     3181 gcggatctcg cgccggcccg agccgtcgac ctcacccacc gccacgatga ccccggcacg
     3241 caccgccacg tcggccgtcc gttccgggct gccggtgccg tcgaccaccg tgccgccgcg
     3301 aatcaccagc tcgtgctcgc tcatctccac gtctcccgga catttccccc gaccgccgga
     3361 ttctcgcaca tcgccccgtc ttgtctacaa tgcgcgcttt cgcgactaag agcccgccgt
     3421 tgcagcgcgt caccatcatc ctaatcgtcc tctcggggct catccttgcc ctgtttgcct
     3481 ggtttccggc ggaaaccctg ctggacgccg cgagagcgct ggaccgcacg ccgcccgagc
     3541 cggcgacgcc ggacgacgtg acccggcgga ccaccgaaag cggggacgtc gtcgggttca
     3601 tcggcaacca cggcgcgcgc acttggcgcg gcgttcccta tgcccgcccg cccgtgggcg
     3661 cgctgcgctg gcgtgcgccg gagcccgccg agccgtggcc cggcgttcgc gaggccaccg
     3721 ctgccggacc gctgtgcgcg cagagagcgg gggtgatctc cggcgacggg ttcgcgccca
     3781 cccgggtggc ggcgggcagc gaggactgtc tgtatctgga catctacgcc ccggccaacg
     3841 cccggcgcct gccggtgatg ttctggatac acggcggcgg caactccgtg gggcacaacg
     3901 gcccctacga cggcgcccgt ctggccacct ggcgcgacgt ggtggtggtc acggtgaatt
     3961 accggctcgc ccacctcggc tggttcgccc accccgccct gaccaccggc aaccggctgc
     4021 acgactcggg caactacggc accctggacc tcatccaggc gctgacctgg gtccgcgaca
     4081 acatccaggc gttcggcggc gacccggaca acgtcaccgt cttcggcgag tccgccggcg
     4141 gcaccaacac gctcagcatg ctggcctcgc cccgggcggc cggcctgttt caccgggcca
     4201 tcgcccagag cggcgggctc acgcccatgc ccatggcccg ggcaagcgca tacgccgagg
     4261 acggcggcca ccccagcagc gctcgtgaga tcgtcaaccg cctgctgatc gccgacggcc
     4321 gcgcaagctc cccggaggac gccaggcggg ttcaggagaa catgccggcc ggcgccatcc
     4381 gcgactatct ttacggcaag agccttaccg agctgttcgc cgtgttcgat aacgggttcg
     4441 ccgggatgat cgacacgccg gaggccctcg gcgacggcca cgtgcttccc gatggtccgc
     4501 tggccgacgt gttcggcgac ccctcgcgcc acaacgccgt gcccgtgatc ctcggcagca
     4561 accgggacga gcccgccctg ttcatggccg cgagccctga gcacgtgaat acctatctgt
     4621 ggatcttccg caggctgaag gacgaggccg gctaccggcg ccaggtgaaa tatctcggcc
     4681 aggcgtggaa ggcacgcggc gtcgatgagt tcgccaacgc catggcggcc gccggcaacc
     4741 cccacgtcta cgcctaccgt ttcgactggg acgaggagcc aagcgtgttg ggcttcgatc
     4801 tgagcgtcgc cctcggcgcg gcccacgctc tggagatccc cttcgtcttc ggttccttct
     4861 cgggcgggct ggggctcggc tacctctacc acgaaagcga aggccgccac gcgcttgccg
     4921 agagcatcac ttcctactgg acggagttcg cctacaacgg cgacccgggc acgggacggg
     4981 acggcggtga ggtccgctgg ttgccctggg gccggaacgg cgccacgtcc atcgtgctgg
     5041 acaccctcga ggataagggc atctacatga ccaccgagcg cgtgaccaca gactcggtga
     5101 aaaccgccat cgcgacggac ccggacgtcc ccgatcagcg cgagcgctgc ctgctctact
     5161 accgggcttt ccgatgggga tcgcagttcg acgtcgatga gttcgccgga ttcggcgacg
     5221 gcggctgcgc cgcctacgac cccgctgccc tgggcgactt ctgaccgatc tccaggggag
     5281 gcggcgtcag cggccggcgg aatcggccgg ctgcggaaac agccggcccc gggcgatctc
     5341 gttgccgggg gccgggcgct cggggatcag ctgggagagc agcagcgagc agacggcgaa
     5401 accggcgccg atgagaaaga ccagggcgtt ggagtggatc cagagcaggc ccagagcggc
     5461 cggcaccacc accgcggcga tgtggttgat ggagaaggac accccggcgg agctggcaat
     5521 atccgccgga tcggccacct tctggaagta ggtgctcatg gcgatggcca gcgcgaagaa
     5581 catgtggtcc accacgtaca gaccggctgc cacgtacggg tccgacacga aggcgtagcc
     5641 cacgaacacg ccgatgaggc cgatgtactc gaaggtgagc gccgcccgct cgccgaagcg
     5701 gccgatgagc cggccgatgc gctcggcgaa cgcccagttg aaggcgtagt tgatcaggaa
     5761 cagcagcgtc acctgggccg ccgagtagcc gaacttctcg accatcagaa acgccgcgaa
     5821 gaccacgaag atctgccgcc gggcgccgga gaagaacgtc agcgcgtagt agagccagta
     5881 gcggcgccgc agcaccaggc gcttcgtctg gggcgttttc gtctcgaagt ggggaaacca
     5941 cagccacatg accacggcca gccccacgca gacgccaccc gagatcagaa agttccagac
     6001 gtagtccagc cggaacagct cgagcaacac ccatagcacg ccgtaggtca ccagcgaggt
     6061 gagcgacccc acggcaagca gccgtcccag catgccgggc gcctcgtccc ggtgcagcca
     6121 ctgcagggaa agggactgct tgagcgtctc gaagtaatga aagccggtgg acatgaccac
     6181 ggtggtgaaa taaagaccgt actccgaagg cagaaagccg gtaagcgcga cgccgagccc
     6241 cagcatggcc agtgacagca cggcaaacgt ctgctcgcgc accacgagga gcacgaagat
     6301 ggtggtgaac gccaggaaac ccggcacctc gcgcaggctc tgcagaatgc cgatctccac
     6361 gccggtgaag gccgcacgct ccaccacgaa gttgttgagc agggcagacc aggcgttgaa
     6421 cgcgacaggc atggccaccg acatcagcat cagcagcatc agcgggctgc gccatcctgc
     6481 ctggaacgtg gtggagacgg ttgtgggtcc tcgggccaat gcggtgcggt ccagtgatat
     6541 gcccggctgg gcgaaggggc gattctaaag gaggcgccgc cggaactcga cgccatgaac
     6601 gggcccagaa accgggccct gatggccgca ggggcccgaa taagccgggg ttgcccccgg
     6661 aatcgttatg gtgttatggt tatgtataac actactgcac ataaagagcg acggccccgt
     6721 gcccgactgg aaggacaacg agcacatcta tcttcagctg aaggacctgg tcctgcggca
     6781 gatcatgctg ggcacgctgc tcgaaggcga gccggtcccc tcggtgcgcc aggtcgcaag
     6841 ccaggagcgc atcaatcccc taaccgtctc ccgcgcctac cagatgctgg tggacgaggg
     6901 ccttttagaa aaacgccgcg gcgtgggcat gttcgtctgc gagggtgcca gggagcaggc
     6961 cctgaaacag gagagaacgc gttttctcga agaggattgg cccgaggtgg tcgagcgcat
     7021 cgccctgctg ggcctgaccc gggacgagct cctgaacacc gctgccgagg agggccgcca
     7081 atgagcctca tcaccgcccg cggtcttgcc aaatccttcg gcgacgtgcg cgccctggac
     7141 ggcctcgacc tcaccgtgga ggccgggcgc atcgtcggcc tgatcggccc caacggctcc
     7201 ggcaagacca ccgccttgaa agccatcctc ggcctgtctc acctcgacgc cggcaccctc
     7261 gacgtactcg gcatgaaccc ggcgaccagc cggaaagcgc tgatgcagcg aacggcctac
     7321 atcgccgacg tgggcatatt gccccgctgg atgcgcgtct cccagctgct ggacttcgtg
     7381 gctggcgtgc atcccggctt ccgccgcgag gtagcggaag cccatctcga atccaccgac
     7441 gtccgtccgg acaaacgaat aaacgggctg tccaagggca tgaacgtcca gctgcacctg
     7501 gccctggtgc tggccatcga cgccgagctg ctcgtgctgg acgagccgac cctggggctc
     7561 gacatcatct accggcagaa attctacgac gcgctgctgg gcgactactt cgacggcaat
     7621 cgcagcattc tggtcaccac ccacgaggtc cgcgagatcg agcacatcct cacggacgtg
     7681 gtgttcattc accacggcag gagcctgttg agcctctcga tggaaagcat ccacgacgag
     7741 ttcgccaagg tggtcgctca accgacgcaa gccggcgcgg tgaggtccct cgggcccatc
     7801 gcggagcgca ccaccttgag cggtgccgag ttcgtgtttc gcggcgccga cagggacgcc
     7861 ctcggcagct acggcgaggt cagcacgccg tctcttcccg agctcttcat cgccatcatg
     7921 ggagataacg catgagcacc atggttgccc tgatcaaacg cgaatggcag gagcatcagg
     7981 tgggcctcgg ctggtccccg gtggccatcc tggccgtgct cgcgctcatc accttcatgg
     8041 ccctggcgac gtcgaccgtg ggcggggtca acgtctccgt ggagggcagc gaaggcagcc
     8101 acagctttca cgaggaagtg gagatctcga gcctctctgc gctcgcgcag ttcatggagc
     8161 tcgaccggtg gtcggacgcc gagctcgagg agaacctcgc caagttccgc agcctggtgg
     8221 cgatgccgtt caaggccgtg tacttcctga tcgccgtgtt cgtcctgatc tccgccctgc
     8281 acgacgaccg gcgcgaccgc agcgtcctgt tctggaaatc catgccggtg agcgatgccg
     8341 agacggtgct cagcaagctc gtcaccgtgg tctgggtcgc ccccgtcgtc gtcatcgcgg
     8401 gcatcgtggc cgcgcagctc ttccttctca tcgtcatctc caccctgacc ctggtggagg
     8461 acgtcggcgg cgcggggcgg ctgtggtggc attccggcct gctgaccgga accgtggagc
     8521 tcgtggtcgg ctacgtgatt cagggcttct gggccctgcc cctgtacgcc tggctgctgg
     8581 ccgtgtccgc ggcggcgccc cggctgccga tcctgtgggc cgtgctcctg cccggcttgc
     8641 cggccattgc cgagcgcatg gtgctcggca cccatggggt aagcgacttc gttcgcgccc
     8701 atctggagtt cgcagcactt ccggcggccg ccagcgagga cgacggaatc atccagcccg
     8761 ccgtggggct ggccgagcag ctctcgctgg tcttcagctt ccagatgtgg ctgggggtcg
     8821 tcgtgggcgg cctgcttctc tggctcgccg tgtacttgcg caaccgcaac aacgacctct
     8881 gaagacgacc tctgaagacg acctcggaga acgacctgca aagacgcctc tggtaagctc
     8941 gccttcttca ccagggagga cgcacgccat ggcatgggat ttctcgaccg agcccgagtt
     9001 tcagaaagag ctggactgga tggacgagtt cgtccgcgag gagatcgaac cgctcgactt
     9061 catcatcggc caccccaacg acttctccga ccccaaccgg gccaagctca ttccgcccct
     9121 gcaggaagag gtgaagaagc gcggcctgtg ggcctgccat ctgggccccg agctcggcgg
     9181 ccagggctac ggccaggtga agctggggct gatgaacgag atcctcggcc gggccaaatg
     9241 cgcacccgtg gtcttcggct gccaggcgcc ggactcgggc aacgcggaga tcctggcgca
     9301 ctacgggacc gagcagcaga agcagcgctt cctgcagccg ttgctcgacg gcgagatcgt
     9361 ctcctgctac tcgatgaccg agccccacgc cggcgccgac ccgaccatgt tccgcacgcg
     9421 cgccgtgcgg gacggggac
//
LOCUS       DBNHKV010000138         5523 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1790193, whole genome shotgun sequence.
ACCESSION   DBNHKV010000138 DBNHKV010000000
VERSION     DBNHKV010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1790193"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="AC16PP_04540"
     CDS             <1..1218
                     /locus_tag="AC16PP_04540"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6480926.1"
                     /translation="FRRNDILKPMHHYLLGTASWFTAFGIQGVMFAWLVTMVLRESPE
                     MVGLAQMTLLLPGTLLMLIGGTYADRFGGRRIVIGAQSVAACAPLLLLVLIWSDMLTY
                     ASMLVYAVFMGCATAFVTPARDGLLNEIAEGRVQRAVMLASMIQFGGQMIGFTLAAGA
                     DLVGPLVVLGAQSLCLALGVLAFVLIPRPHEGRHVRTGRLLASLAEGARTVFSSASMR
                     LIVVQNVAMAIFFMGSYVVAMPLLVREVFNGTARDLAFTNGSNSLGLVLSIVLMLRLG
                     DVTRQGRALVLSQLAGGLMLIAAALMTSFALYVLMLFIWGACGGIAMTMARTIMQEQA
                     PDAQRSRVMSFYALSFMGAGPLGALFTGYMVELLGPQQALVLSGSLMAVVACILAVGS
                     NLWSLRAYQVASA"
     gene            1220..3322
                     /locus_tag="AC16PP_04545"
     CDS             1220..3322
                     /locus_tag="AC16PP_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR6480927.1"
                     /translation="MSRRDATYRLAVDIGGTFTDVVYEPDAAGAQRVTVKVLTTYDDP
                     ANGVMQGIEAVLERAGAEAGDAGLVLHGTTLATNALIERRGARTALLTTEGHRDALEM
                     ALENRFEQYDVNIDRPRPLVPRRLRLPVRERLDAAGRVLVPMDSKSLDAAVAALDGHG
                     VESVAIGFLHAYANPDHEHAAAERVREKLPDVSITLASQVCPEIREYERLSTACANAY
                     VKPLMARYLGSLQRKLTDRGMVCPVLLMTSGGGLTTLETAAEFPIRLVESGPAGGALL
                     AARIAAEGGHERVLSFDMGGTTAKICLIDGAAPLLSRSFEVDRTYRFKKGSGLPVRIP
                     VIEMVEIGAGGGSVARVDKLSRITVGPDSAGSEPGPACYGRGGRDATVTDGDVVMGRL
                     DPKSFAGGRLALDTAAAERAVTEAIGDPLGLNLHTAAFGISEVVDENMAAAARAHAVE
                     WGEDTQGRTLVAFGGAAPLHAAQLAVKLKLDRVIVPAHAGVGSAIGFLIAPVSYEVVR
                     SRYMRVSGFDADLVNAVMAEMRAEALEVVSSAAETEGLLETRRAYMRYVGQGYEIAVP
                     VPGGTIAEDAGEVLRAAFDHEYRRLYRRTIPNLDVEVLSWSLALAAPQPEFSADSAAP
                     RTDPAEAVRRVSLFEPLTGEMADGAFYRRVDIRPGHWLEGPAVISEEQTTTVVPAGFS
                     AGVTVRGHLVLERMETET"
     gene            3331..5019
                     /locus_tag="AC16PP_04550"
     CDS             3331..5019
                     /locus_tag="AC16PP_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR6480928.1"
                     /translation="MSELRTQLVWNRLLAIVEEQAQTLMRTAFSTVVREAGDLSAGVF
                     NTHGDMLAQAVTGTPGHVNAMAASVGKFLKRHPVETLRPGDVLLTNDPWDGTGHLNDF
                     TAVTPVFLADACVGLFASTSHIADVGGRGFGPDANDLFEEGLRIPITFLFREGVRDET
                     LMMLVAANVRDPVVAEGDLYSLTACNDTGARQLVELMDDFGLADLEEVGREILEKSRR
                     AMMAEIRNLPPGEYRHTMRIDGYDRPVDLACTLTVSGSGIDIDWTGTSPVSAAGINVP
                     LPYTEAYASFGVRCVVGNDVPNNAGSLDTIRVTAPRGCILNAEPPRAVSARHAVGQML
                     PDVVLGCLAQVVSDRVPAEGASCIWNPVFLNAPSEDGGNAPELQFVTNPIYNGGTGAR
                     PGKDGLSTTAFPSGVRTTSTEVNEATAPLLIWRKEYRQDSAGPGEHRGGLGQTVEVAH
                     ARGESFVISKMFDRIRHPARGRNGGGDGEPGRVYVKGGDDFRGKGRDVVPPGATLVME
                     TPGGAGMGRVAERDPDRIAADVEAGLVSPDAARDRYGLDAGVRQNDDEGGAADV"
     gene            5090..5165
                     /locus_tag="AC16PP_04555"
     tRNA            5090..5165
                     /locus_tag="AC16PP_04555"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5123..5125,aa:Gly,seq:gcc)
BASE COUNT          842 a         1739 c         1938 g         1004 t
ORIGIN      
        1 tttcgccgca acgatatctt gaagcccatg catcattacc tgctcgggac cgcgagctgg
       61 tttaccgcgt tcggtatcca gggcgtcatg ttcgcgtggc tggtcacgat ggtgctgcgc
      121 gaatctccgg agatggtcgg tctcgctcag atgaccctgc tgctcccagg gaccctcctg
      181 atgctgatcg ggggcactta tgcggaccgc ttcggaggcc ggcgcatcgt catcggcgcg
      241 caatccgtgg cggcctgcgc gccgctgctt ctgctcgtcc tgatctggag cgatatgctc
      301 acttacgcca gcatgctggt ctacgcggtg ttcatgggtt gcgccaccgc tttcgtcacc
      361 ccggcccgcg acggtctgct caacgaaatc gcggagggca gggtccagcg cgcggtcatg
      421 ctggccagca tgatccagtt cggcgggcag atgatcggct tcacgctcgc tgcgggcgcc
      481 gacctggtgg gtcccctggt ggtgctcggc gctcagagcc tctgcctggc gctcggcgtg
      541 ctggcattcg tgctgattcc gcgtccccac gaggggcggc acgtgcgcac gggccggttg
      601 ttggcttccc tggccgaggg tgcccggacc gtcttctcgt ctgcgtcgat gcggttgatc
      661 gtggtgcaga acgtggcgat ggccatcttc ttcatggggt cttacgtggt ggccatgccg
      721 ttgctggtcc gggaggtgtt caacggcacc gcccgggacc tggcctttac caacggctcg
      781 aactctctcg ggctggtgct cagcatcgtc ctcatgctcc ggcttggcga tgtgacccgt
      841 cagggccggg cgctggtgct gtcgcagctg gcaggtggcc tcatgctgat cgccgcggcg
      901 ctgatgacga gctttgctct ttacgtgctc atgttgttca tctggggcgc ctgtggcgga
      961 atagcgatga ccatggcaag aaccatcatg caggagcagg cgcctgacgc ccagcgcagc
     1021 cgggtgatga gcttttacgc gctctcgttc atgggggccg ggcccctggg agcgctcttc
     1081 acgggctaca tggtggagct gctcggcccc cagcaggcgc tcgtcctgtc gggcagcctg
     1141 atggccgtgg tggcctgcat tctcgccgtg ggctcaaatc tctggtctct gagagcttat
     1201 caggtggcgt ctgcctgaca tgagcagaag ggacgcaacg taccggttgg ccgtggatat
     1261 cggtggtacg ttcaccgacg tggtttacga gcccgatgcg gcgggtgcgc agcgcgtcac
     1321 cgtcaaggtg ctgacgacct acgacgatcc tgccaacggc gtgatgcagg ggattgaagc
     1381 cgtgctggaa cgcgccggcg ccgaggcggg cgacgcgggg ctcgttcttc atggcaccac
     1441 gctggcgacc aacgcgctga tcgagcgccg aggcgcgcga acggcgcttc tgaccaccga
     1501 gggtcatcgc gatgccctgg agatggcgct ggagaatcgt ttcgagcagt acgacgtgaa
     1561 catcgaccgg ccgcggcccc tggttccccg gcgcttgcgc ctgcccgtcc gggaacgtct
     1621 ggacgccgct ggcagggtgc tggtaccgat ggattcaaag tctctggacg ctgcggtggc
     1681 cgccctggac gggcacgggg tcgagagcgt ggcgatcggt ttcctgcacg cctacgcgaa
     1741 ccctgatcac gagcacgccg cggccgagcg cgttcgcgag aagctgcccg acgtctcgat
     1801 cacgctggcc tctcaggtct gtcccgagat ccgcgagtac gagaggttgt cgacggcgtg
     1861 cgccaacgcc tacgtcaagc cgctcatggc ccgctatctg ggcagtctgc agcgtaagct
     1921 cactgatcgc gggatggtct gccccgtcct cctgatgacg tcgggcggtg ggctcaccac
     1981 cctggagacg gcggcggagt ttcccatccg cctggtggag tcgggacccg caggcggggc
     2041 gctgctcgcc gcccgaatcg cggctgaggg tggccatgaa cgggtgctgt ccttcgatat
     2101 gggcggaact acggccaaga tctgtctgat cgacggcgcc gcgccgctgc tttcccggtc
     2161 cttcgaagtg gatcggactt accgcttcaa gaagggaagc gggctgccgg ttcgaattcc
     2221 cgtcatcgag atggtcgaga tcggcgccgg cggcggctcg gttgcccggg tagacaagct
     2281 gtcgcgcatc acggtgggcc cggacagcgc ggggtccgag cctggacccg cctgctacgg
     2341 gcgtggcggg cgcgacgcga cggtcaccga cggcgacgtc gtcatgggcc gtctggatcc
     2401 gaagtccttc gccggcgggc gcctcgccct ggataccgcc gcggccgagc gcgccgtgac
     2461 ggaagcgatc ggcgacccgc tcgggctcaa cctgcacacg gccgcgttcg gaatcagcga
     2521 ggtggtcgac gagaacatgg cggctgcggc ccgggcccat gcggtggagt ggggcgaaga
     2581 tacccaggga cggacccttg tcgcctttgg cggagcggca ccgctccatg ccgcccagct
     2641 cgccgtcaag ctgaagctgg atcgggtgat cgtgccggcc cacgcgggtg tggggtcggc
     2701 gatcggtttc ctgatcgctc cggtcagcta cgaggtggtg cgcagccgtt acatgcgcgt
     2761 atcgggtttc gatgcggacc tggtcaacgc cgtcatggcg gagatgcgtg ccgaagccct
     2821 ggaggtggtg tcctccgcag cagagacgga agggctgctg gaaacccgtc gcgcttacat
     2881 gcgctacgtg ggccagggct acgagatcgc tgtgcccgta ccgggcggga cgatcgccga
     2941 ggacgcgggc gaggtcctcc gagcggcctt cgaccacgaa taccggcgcc tgtaccggcg
     3001 caccattccc aatctggatg tcgaggtcct gagctggtcg cttgctctcg ccgcgcccca
     3061 gccggagttt tcggccgact ccgcggcccc gcgaacggac cctgcagagg ccgtgcgcag
     3121 ggtctccctg ttcgaaccgc tgacggggga aatggccgac ggcgccttct atcgacgggt
     3181 cgacatccgg cccggccact ggctggaggg acccgccgtg atctccgaag agcagacgac
     3241 caccgtggtg cccgccgggt tctcggcagg ggtcacggtc aggggtcacc tggtgctgga
     3301 acggatggag accgagacgt gagtgcgaac gtgagtgaac tgcggaccca gctggtctgg
     3361 aaccggctgc ttgccatcgt ggaggagcag gcccagaccc tgatgcgcac cgcgttttca
     3421 acggtcgtgc gggaggccgg cgacctctcg gccggggtgt tcaacaccca cggcgacatg
     3481 ctggcccagg cggtcaccgg cacgcccggt cacgtcaacg ccatggccgc gtcggtcggc
     3541 aaattcctga agcgtcatcc cgtggagacg ctgcggccgg gtgacgtgct gctcaccaac
     3601 gacccctggg acggaaccgg tcacctcaac gatttcacgg cagtcacgcc ggtctttctc
     3661 gcggatgcgt gcgtaggcct gtttgcgtcc accagccaca tcgcggatgt cgggggacga
     3721 ggcttcgggc ccgatgccaa cgacctgttc gaggaagggc tgcgcattcc catcactttt
     3781 ctgttccggg agggggtgcg cgacgagacg ctcatgatgc tcgtcgccgc gaacgtgcga
     3841 gatccggtcg tggcagaagg cgacctctat tcgctgaccg cctgcaacga cacgggcgcg
     3901 cgccagctcg tggagctgat ggacgacttc gggctggccg acctggagga ggtggggcgc
     3961 gagatactcg aaaagtcccg tcgtgccatg atggccgaga tccgcaacct tccgccgggc
     4021 gagtaccggc acaccatgcg gatagacggt tacgacaggc ccgtggatct ggcctgcacg
     4081 ctgacggtca gcggcagcgg gatcgacatc gactggacgg gcacctcgcc cgtttccgcg
     4141 gcgggcatca acgtgcctct accctacacc gaggcctatg cgtcattcgg cgtgcgctgc
     4201 gtggtgggca acgacgtgcc gaacaacgcg ggttccctgg acaccatccg ggtcaccgct
     4261 ccccgaggct gcatcctcaa cgcggagccg ccgcgcgcgg tctcggcgcg tcacgccgta
     4321 ggtcagatgt tgccggacgt ggtgctcggc tgccttgccc aggtggtttc ggaccgggtg
     4381 ccggcggagg gcgcgtcgtg tatctggaac cccgtgttcc tgaacgctcc ctcggaggac
     4441 gggggcaacg caccggagct gcagttcgtt accaacccca tctacaacgg aggcaccggg
     4501 gctcggccgg gcaaggatgg tctttccacc acggctttcc cttcgggggt gcgcaccacg
     4561 tcgacggagg tcaacgaagc aacggcgccg ctgctgatat ggcgcaagga gtatcggcag
     4621 gactcggctg gtccggggga gcatcgcggc gggctggggc agacggtgga ggttgcgcat
     4681 gcgcggggag aatctttcgt gatctccaag atgttcgatc gcatccggca tcctgcccgg
     4741 ggtcgaaatg gcgggggaga cggcgagcct ggtcgggtct acgtgaaagg gggagatgac
     4801 ttccggggca agggcaggga cgtggtgcca ccaggcgcca cgctggtcat ggagaccccg
     4861 ggcggggccg gcatgggcag ggtggccgag cgcgaccctg accggatcgc cgccgatgtc
     4921 gaggccggtc tggtcagccc cgatgcagcg cgcgatcgct acgggctcga cgccggggtc
     4981 cggcagaatg acgatgaagg cggcgcggca gacgtctagc gcttccactc ggcgggccga
     5041 ttcggctata gttcgcgtcc ggccgtcgga ccggacggat tcttccaggg cgggaatagc
     5101 tcagttggta gagcacaacc ttgccaaggt tggggtcgcg agttcgagtc tcgtttcccg
     5161 ctccacttca tttcgacgcg ttcgtcgatt ctttctccca tgcacgccga tgatgttgat
     5221 gagagggcgg tttcccgaga cagtcgagaa accatgaatt tccgcaacct cgtcaatttc
     5281 gacttcagta aacgtttgcc cagggagttc gggggcgagc ttatgtacgt cacggcgcgg
     5341 tccgacatcc gcgtacaggg ttggtccgca gggcgccgtt tacgcgctgg aagccgatcc
     5401 ggcttacgca gatcggataa tccgctcgag ccgccgcctg tcatcggcct accagccggt
     5461 caacgttctg tgcgccgcca tcgccgatca cgcgggggtg ctggagttcg ctgttgtgaa
     5521 aca
//
LOCUS       DBNHKV010000139         4054 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_373075, whole genome shotgun sequence.
ACCESSION   DBNHKV010000139 DBNHKV010000000
VERSION     DBNHKV010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_373075"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="AC16PP_04560"
     CDS             <1..655
                     /locus_tag="AC16PP_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6480929.1"
                     /translation="RRYEIPVIEDNCYGDVHYEGPVEPALYALDDDPNQIYLCSLSKI
                     LAPGLRLGYLIARPPMLERILARRHDAGSNTLAAAIAAEFYRDGIWDHTRYANAALKE
                     KRDLLFDRLQAELDDVCVWSRPVGGLFVWVRIPDDINRKQLYELAQSRGFNYLPGISF
                     HVETRNVPYLRLAFGHLTLDQIRDGIPVLAQCIRECRTSNEPRGFDSLFQDREGSSA"
     gene            complement(628..1020)
                     /locus_tag="AC16PP_04565"
     CDS             complement(628..1020)
                     /locus_tag="AC16PP_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480930.1"
                     /translation="MYRALTEEVHAWWDAANSYSGDAANFYLEPRALGCFCEKLPGGG
                     SIAHMQVVYVEPGAVLRMLGGLGPLQGMGATGAVEFRLSAAADGARVEYRYHVTGFGA
                     ESMAEPVDGVQLGQFERLKRYAEEPSRS"
     gene            complement(1045..1431)
                     /locus_tag="AC16PP_04570"
     CDS             complement(1045..1431)
                     /locus_tag="AC16PP_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HIR6480931.1"
                     /translation="MECPKCPGTLETKTYGRKITVHRCSDCGGLWCKPEVLLEMKREW
                     MADVILDSGDPKLGQALDKLDNISCPECGVKMEKKTDDRQTHIWYESCPQCEGMFFDA
                     GEFTDLKYDTFMDRIRGALRGRRPSA"
     gene            complement(1443..1946)
                     /locus_tag="AC16PP_04575"
     CDS             complement(1443..1946)
                     /locus_tag="AC16PP_04575"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6480932.1"
                     /translation="MPALSIAERDDFLAERGIVMRIGVVRADGSPLVTPIWFLHEDGA
                     IFFTPREKSEWFACLRRDPRVSLCIDEQPLPYRKVIVEGRAELVHDVGEDDVWRDLYR
                     RIAKRYVDDAEAEAYVQNTIDQPRGLYRVVMSEGEIKTWRMPVAGEAPEGIWHSRYYK
                     PDTKFNR"
     gene            complement(1967..3217)
                     /locus_tag="AC16PP_04580"
     CDS             complement(1967..3217)
                     /locus_tag="AC16PP_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6480933.1"
                     /translation="MNVKPVPTLDEEVNEIRLATAEIVNEDILPNEDRLWGWLADSAN
                     ATNEVKQEAKALRVDIQEKVKNARLWAPHLPREFGGMGLTFMQHAYMNEVLAYSPGAA
                     SLFGVVAPNSGNQKILVKYGTPDQHEKWLKPLTDGTAQSGFSMTEPHNPGSDPRSITT
                     RAELDGDEWVINGHKWFTSNGFAADFYIVMCRTSDPDDPGGANEKMTQIIVPRKTPGV
                     NIVRGVPVWGRASSHCEIVYDNVRVPKENQLGRTGTGHQAAQDRLGAGRVYHCMNSIG
                     SMWRAFDLMIDRLMTREVHGGEKLEDKQFMQGFVADSYIDIQTARLMTIHCAEKMESG
                     ADPRTDISAIKIFVPQAYHRVVDRAIQVWGAAGVSGDLPLASMYQGARTLRLADGPDE
                     VHRILIAKNILRRYHAGESWDFGN"
     gene            3373..>4054
                     /locus_tag="AC16PP_04585"
     CDS             3373..>4054
                     /locus_tag="AC16PP_04585"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6480934.1"
                     /translation="MGTHELNYPHAEHPAPGTTVELAPGVRWLTMPMGGSLNHINLYL
                     LEDTTGWWIVDTGLSLDQTRELWHEIFSRELQGRPVLGVICTHFHPDHIGQSKMITDE
                     FRCSLYMTRAEYYQARAFSGSGPTYQSSWLGETFYQRAGMPADYLEQLREMWAHRSSE
                     GMSMPDMPAGYRRLEDGDVLTIGGHDWRIVVGSGHSPEHACLYCAALKIMISGDQILP
                     VITSNVSVH"
BASE COUNT          733 a         1359 c         1191 g          771 t
ORIGIN      
        1 gagacgctac gagattccgg tcatcgagga caactgctac ggcgacgtgc actacgaggg
       61 gccggtggaa ccggcgctgt atgccctgga tgacgatccc aaccagatct acctgtgctc
      121 gctgtcaaag atcctggccc ccggactgcg gctgggatac ctcattgccc gaccgccgat
      181 gctcgaacgc attctggcca ggcgtcatga cgccggcagc aacacgctgg cggcagccat
      241 cgccgcggag ttctaccggg acgggatatg ggaccacacc cgctatgcca acgccgcact
      301 caaggaaaag cgcgatctgc tgttcgaccg tctccaggca gagctggacg acgtctgcgt
      361 gtggtcgcga cccgtcggcg ggctcttcgt gtgggtccgg atacccgacg acatcaaccg
      421 caagcagctc tacgagctcg ctcagagcag agggttcaac tacctgcccg gcatcagctt
      481 tcacgtggag acgcgcaacg tgccctatct gcggctagct ttcggccacc tgaccctgga
      541 tcagatccgc gacggcatcc ccgtcctggc gcagtgcatc cgggagtgcc gcaccagcaa
      601 cgagccgcgc ggcttcgatt ccctgtttca ggatcgggag ggctcttcgg cgtagcgctt
      661 caggcgttcg aactggccca gctggacccc gtccacgggt tcggccatgg attcggcacc
      721 gaacccggtc acgtgatacc ggtactcgac ccgggcgccg tcggcggccg cggacagacg
      781 gaactccacg gcgccggttg cacccatacc ctggagcggg ccaagcccgc ccaacatccg
      841 cagaaccgcg ccaggctcga cgtagaccac ctgcatgtgg gcaatgctgc cgccgccagg
      901 cagcttctcg cagaagcacc cgagcgcgcg aggttcaaga taaaagttag ccgcgtcgcc
      961 ggagtaagaa ttggccgcgt cccaccaggc atgcacctct tcggtgagag cgcgatacac
     1021 gcgctcgggc ggcgcatcgc cggttcaggc gctcgggcga cgcccgcgca gagcgccgcg
     1081 aatccggtcc atgaaggtgt cgtatttcag gtcggtgaac tcgccggcat cgaagaacat
     1141 gccttcgcat tgcgggcagc tttcgtacca gatgtgcgtc tgccggtcgt cggtcttctt
     1201 ctccatcttc acgccgcact cggggcagct gatgttgtcg agcttgtcca gcgcctgacc
     1261 gagcttggga tcacccgagt cgagaatgac gtccgccatc cactcgcgct tcatctcgag
     1321 aagcacctca ggcttgcacc acaagccgcc gcagtccgaa caccgatgca cagtgatttt
     1381 gcggccgtag gtcttcgtct cgagcgttcc gggacatttc ggacattcca tgaacagatc
     1441 tcctaccgat tgaatttcgt atccggcttg taataccgac tgtgccagat tccttccggt
     1501 gcttctcctg ccaccggcat tctccaggtc ttgatttcac cttcgctcat cacgacgcga
     1561 tacagcccgc ggggctgatc gatcgtattc tgcacgtaag cttccgcttc cgcatcgtct
     1621 acgtaacgct tcgcgatccg ccggtaaaga tctcgccaga catcgtcctc gccgacgtcg
     1681 tgcacaagct ccgcgcgccc ctcgacgatg actttgcgat acggcaacgg ctgctcgtcg
     1741 atgcacagcg aaacccgcgg gtcgcgccgc aggcaggcaa accattcgga tttctccctg
     1801 ggagtgaaaa atatcgcccc gtcctcgtga agaaaccaga tcggggtcac cagcggcgag
     1861 ccgtcggcac gaaccacgcc gatcctcatg acgatcccac gctcggcaag aaaatcgtcg
     1921 cgctctgcaa tagacagtgc gggcacgccc cctccccctt tagttcctag ttgccgaaat
     1981 cccaggactc gccggcatgg tatcggcgca gtatgttctt ggcgatgagg attcgatgga
     2041 cctcgtccgg tccatccgcc aggcgcaggg tgcgcgcgcc ctgatacatg ctcgccagcg
     2101 gcagatcgcc cgacacgccc gcagcgcccc agacctgaat cgcgcgatcc accacccggt
     2161 ggtaagcctg aggcacgaat atcttgatgg cggaaatgtc cgtgcgcgga tccgctccgc
     2221 tttccatctt ttcagcgcaa tggatcgtca tcagcctggc ggtctgaata tcgatgtagc
     2281 tgtcggcgac gaagccctgc atgaactgtt tgtcttccag tttctcgccg ccgtggactt
     2341 cccgcgtcat gagacggtcg atcatcaggt cgaacgcgcg ccacatggag ccgatggagt
     2401 tcatgcagtg ataaacgcgg cccgctccga gccgatcctg ggccgcctga tgcccggtac
     2461 cggtgcggcc gagctgattc tccttcggca cgcgaacgtt gtcgtagacg atctcgcagt
     2521 gactggacgc tcggccccac accggtactc cgcgcacgat gttcaccccg ggagttttgc
     2581 gcggcacgat gatctgcgtc atcttctcgt tggcgccgcc gggatcgtcc ggatccgacg
     2641 tccggcacat cacgatatag aaatcggccg caaacccgtt cgaggtgaac cacttgtgcc
     2701 cattgatgac ccattcatcg ccatcgagct ccgcccgggt cgtgatcgag cgtggatctg
     2761 atcccgggtt gtgcggctcg gtcatggaaa agcccgactg ggcggtgccg tccgtcagcg
     2821 gcttgagcca tttctcgtgc tgatccggcg tgccgtactt gaccaggatc ttctggttgc
     2881 cggaattcgg cgccacgacc ccgaacaggg aagccgcacc ggggctgtag gccagcacct
     2941 cgttcatgta cgcgtgctgc atgaaagtca ggcccatgcc gccgaattcc cgcggcaggt
     3001 gcggcgccca gagccgggcg ttcttcactt tctcctgaat gtcgacacgc agcgccttgg
     3061 cttcttgctt gacctcgttg gtcgcgttgg cgctgtcggc taaccaaccc cacaagcggt
     3121 cctcgttggg cagaatgtcc tcattgacga tctcggcggt ggccagccgg atctcgttga
     3181 cctcctcgtc gagcgtcggt accggcttca cattcatcga cacgacaatt cctccctcat
     3241 gcggccagtc gattaatcta attgaacacg gcccgcattg cgactgaccg gtacccgggt
     3301 gccggggccc gctactgcag attgggcttt ttggtatgct gctggatcga ggactgcaaa
     3361 ccgggaacga cgatgggcac tcacgagctc aactatccgc acgccgagca tccggcaccg
     3421 ggaaccacgg tcgagctcgc cccgggcgtg cgctggctga ccatgcccat gggcggctcg
     3481 ctgaaccaca tcaatctgta tctgctggaa gacacgaccg gctggtggat agtggacacc
     3541 ggcttgagcc tcgaccagac ccgcgagctg tggcacgaga tcttctcccg agagctccag
     3601 ggcaggcccg tcctgggcgt catctgcacc cactttcatc cggaccacat cggccagtcg
     3661 aagatgatca ccgacgagtt ccgctgcagc ctctacatga cccgcgccga gtactaccag
     3721 gcccgcgcct tttcgggcag cgggccgacc tatcagtcca gctggctggg cgagacgttc
     3781 taccagcgcg ccggcatgcc cgcagactac ctcgagcaac tccgcgagat gtgggcgcac
     3841 cggtcttcgg aaggcatgtc gatgcccgac atgccggccg gctaccggcg cctggaggac
     3901 ggggacgttc tcaccatcgg cggtcacgac tggcgcatcg tggtgggctc gggccactcg
     3961 cctgaacatg cctgcctgta ctgcgcggcc ctgaagatca tgatctcggg cgaccagatc
     4021 ctgcccgtga tcacgagcaa cgtcagcgtg cacc
//
LOCUS       DBNHKV010000140         8990 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_658225, whole genome shotgun sequence.
ACCESSION   DBNHKV010000140 DBNHKV010000000
VERSION     DBNHKV010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8990)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8990
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_658225"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="AC16PP_04590"
     CDS             complement(<1..933)
                     /locus_tag="AC16PP_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HIR6480935.1"
                     /translation="MSTQIEQQQSRLKELIAKGKEQGYITYSEVNDHLPDDISDPDQI
                     EDIIRMINDMGITVYETAPDADELLSTEENTADELAAEEAAAALAAVETEAGRTTDPV
                     RMYMREMGTVELLTREGEIAIAKRIEEGIRDMLSAMAYFPGTVEYLLEVYSEANAEGR
                     LSDMLVGYLDPTDNVPQAAQVQPGAQNQKDDDDEEEDKGPDPVEAKRRFTALKRQYNK
                     TYKVIAEKGRNSKEAQAQLEKLGVAFSPFKLVPRHFDELVDMPRMALAIVRRHERIIM
                     NACVRQARMPRAVFRKEYVGSETSVAWVNKHIKAG"
     gene            complement(1134..2858)
                     /gene="dnaG"
                     /locus_tag="AC16PP_04595"
     CDS             complement(1134..2858)
                     /gene="dnaG"
                     /locus_tag="AC16PP_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR6480936.1"
                     /translation="MAGRIPQSFINDLLNRVDIVDVIDARLQLRKAGRDYQALCPFHN
                     EKTPSFTVSPEKQYYHCFGCGESGTALTFLMEHDRMEFVEAVEALAQLVGVEVPREQG
                     GRPARDNTDLYDVLAAADRYFRKALRQSPEAIEYLKARGVTGTIARDFGIGYAPDEWQ
                     GAASALAGMGEAKLLEAGLLTRGEKGRAYDRFRGRIMFPIRDTRGRIIGFGGRVMADG
                     GGPKYLNSPETPVFHKGRELYGLYEARQALRRIDRLIVVEGYMDVVALAQAGISNAVA
                     SLGTAATEEHFQSLYRYTGEVICCFDGDAAGRQAAWRALESALPALSDGRGLKFMFLP
                     DGEDPDSLVRARGKAGYLELAARAVPAVEYLFGRLEEGLDLGSVDDKARFASLAMPHI
                     DRVPAGILKELMVSRLEGLTGFRPSAQARPSRGPARRTAADVSLTPLARRLVGYLLRE
                     PSVVSALSEEARRALRPLAGNDLLAELVTYLDEHPDAETPELLGRWTGHDLHDELTGL
                     ARQPSAIDIAAVRGDFTEGVARYLEMQQASARRQLISEMHQDPSAERLKDYWSLKQGP
                     RDKVSGTD"
     gene            2963..3205
                     /locus_tag="AC16PP_04600"
     CDS             2963..3205
                     /locus_tag="AC16PP_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480937.1"
                     /translation="MRTTVRLPGELLTKAKEFAHERGTTLTALIEEGLIMVLERAPKA
                     GKMKPVRVPVSKSSGGLQPGVDLSDSAALLDAMEQG"
     gene            3202..3624
                     /locus_tag="AC16PP_04605"
     CDS             3202..3624
                     /locus_tag="AC16PP_04605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HIR6480938.1"
                     /translation="MKLADVNVLVGAFRTDSSHHERCRSWLKSTVNAPASFGLAAQVL
                     SGVIRVTTHPKVFVNPSPIDESIRFANFLLGRPNAIVVSPGERHWTIFTDLCRSGGIQ
                     GNLVPDAWFAALAIESGCEWVTLNGNFARFEGLSWSAP"
     gene            complement(3631..5055)
                     /locus_tag="AC16PP_04610"
     CDS             complement(3631..5055)
                     /locus_tag="AC16PP_04610"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HIR6480939.1"
                     /translation="MEKVIPVLLAGGTGSRLWPLSRELFPKQFHALLGDDSLLQSTLK
                     RADRTTGEAPIIVCNEEHRFMVAEQCRAIGQSWRRIILEPEGRNTAPAIALAALAACE
                     EAGPDSAPLLLVLPSDHLITDLDGFASAAELAARAAGEGALVTFGVVPTSPETGYGYI
                     EAPGAELGSGVVRVRSFVEKPDLATANEYLAAGDYFWNSGMFLLNARAYLDELGRHSP
                     LMLEKASLAFERGAEDLDFFRPGPEFLDCPADSIDYAVMEKTDRAMVVPAGFDWSDVG
                     SWSAIWEASDRDGNGNRLEGDVISVDTQNSFVSARDRLVGTIGVSDLVVVETSDAVLV
                     ADKDRVQDVRAVVEQLKAAGRDEYVHHREVFRPWGSYESVGAGERYQVKRIKVKPGAS
                     LSLQMHHHRAEHWIVVRGTAEVTRGDETITLAENQSTYIPLGVTHRLANPGKLTLELI
                     EVQVGSYLGEDDIVRFDDIYGREG"
     gene            complement(5170..5637)
                     /locus_tag="AC16PP_04615"
     CDS             complement(5170..5637)
                     /locus_tag="AC16PP_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR6480940.1"
                     /translation="MSDLPISELRQAITAATTAAMKARDKQRLAVLRLVNADIKRAEI
                     DERRAASTDAEVLAVLNRMLKQRQDALEQYTQAGRKDLADQESFEIDLIGEFMPEQLS
                     EAELASAIDQAIAEANAESMRDMGKVMGLLKDRLQGRADMGAVSGRVKALLAG"
     gene            complement(5660..5875)
                     /gene="rpsU"
                     /locus_tag="AC16PP_04620"
     CDS             complement(5660..5875)
                     /gene="rpsU"
                     /locus_tag="AC16PP_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR6480941.1"
                     /translation="MPSVKVKENEPFDVALRRFKRSCEKAGVLSEVRRREYYEKPTSV
                     RKRKAAAAVKRHLKKLQREARRFEKSF"
     gene            5983..7005
                     /gene="tsaD"
                     /locus_tag="AC16PP_04625"
     CDS             5983..7005
                     /gene="tsaD"
                     /locus_tag="AC16PP_04625"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR6480942.1"
                     /translation="MLVLGIETSCDETGVALYDGVEGLLADALHSQVEVHAAYGGVVP
                     ELASRDHIRKLVPLVRKTLDDSGRSLADVDGVAYTSGPGLMGALLVGAGFARSLAWSL
                     GKPAVAVHHMEAHLLAPLMNPDTAPPMPFIALLVSGGHTQLVLVNAIGDYELLGESVD
                     DAAGEAFDKAAKMLDLGYPGGPNLARAAEGGDPHRFTFPRPMTDRPGLDFSFSGLKTY
                     TLNTTRDLAPMSDADRADVARAFEEAVVDTLYIKCRRAVEQTGITHLVMAGGVAANAR
                     LRRKLGEDLRAAVYYPPARLCTDNGAMIAYAGCLRLQAGERAPLAIETRARWPIVELG
                     RPGPPA"
     gene            7009..7362
                     /gene="folB"
                     /locus_tag="AC16PP_04630"
     CDS             7009..7362
                     /gene="folB"
                     /locus_tag="AC16PP_04630"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF014233.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR6480943.1"
                     /translation="MDSVFVRNLTVSTIVGVYPEERTRPQRVIVSFELFADTRAAAQS
                     DDLEDALDYGEAASRVAEFVRGSRFELIEALADGIAALLLEGYPAARVVVEVLKPGAV
                     TDAETVGVKIERSRA"
     gene            complement(7414..8487)
                     /locus_tag="AC16PP_04635"
     CDS             complement(7414..8487)
                     /locus_tag="AC16PP_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA nucleotidyltransferase/poly(A)
                     polymerase-related protein"
                     /protein_id="HIR6480944.1"
                     /translation="MDVYLVGGAVRDELLGRPVRERDWVVVGASPEAMLKLGFRQVGR
                     DFPVFLHPDTSEEYALARTERKTGPGHKGFECHAGPDVTLEQDLERRDLTVNAIARAS
                     DGSLIDPFGGRSDLEAKVLRHVSVAFSEDPLRVFRVARFAAQLDDFTVAEETRALMAE
                     LAGSGALAELSAERVWQELVKALGSKAPLRFFTVLASVGGLSTWFAELAEVEITFPEA
                     IEDALGRFAALGWHLAPEAVDTLCERLRAPRRHRRAMGHIASHGVVLAAWETAEAGSL
                     CAALKSVGAFKAGDDPEMAVRVSAACAGRNPEPLLELAASIRQEVTTASVGGSLTGAA
                     LGEAIDQARTQAIRQAQRSSVGR"
     gene            complement(8505..>8990)
                     /locus_tag="AC16PP_04640"
     CDS             complement(8505..>8990)
                     /locus_tag="AC16PP_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480945.1"
                     /translation="GSVVWLRVPDPEAGRKPTRWQVPESTGFDGGEGMSYCDGVVYLA
                     TKGDNRVWALDLGEDRLEVIYDQATAATPILGGVDNVETNASGDVLVAEDGDDMQIVV
                     LPREGEPVALLQVTRQGGSEITGLAFTPDMSRLYFSSQRGFSGRVTGGITYEVTGPFV
                     G"
BASE COUNT         1522 a         3061 c         2843 g         1564 t
ORIGIN      
        1 gccggccttg atgtgcttgt tcacccaggc aacgctggtc tccgagccca cgtactcctt
       61 ccggaagacg gcgcgaggca tccgcgcctg acgcacacag gcgttcatga tgatgcgctc
      121 atgacgccgg acgatggcta gcgccatccg cggcatgtcc accagctcat cgaagtggcg
      181 cggaaccagc ttgaagggcg aaaacgccac gcccagcttc tccagctgag cctgggcttc
      241 cttgctgttg cggcctttct ccgcaatgac cttgtacgtc ttgttgtact ggcgcttcaa
      301 cgcggtaaag cggcgctttg cctccaccgg atccggaccc ttgtcctctt cctcgtcgtc
      361 atcgtccttc tggttctgag cgcccggctg aacctgagcc gcctgaggaa cgttgtcggt
      421 ggggtcgaga tagccaacga gcatgtcgct caagcgccct tccgcattgg cctcggagta
      481 aacctccagg aggtactcca cggtgccggg gaaataggcc atggcggaga gcatgtcgcg
      541 gatgccctcc tcgatgcgct tggcaatggc gatctcgcct tcgcgggtaa gaagctccac
      601 cgtgcccatc tcgcgcatgt acatgcgcac cgggtcggtg gtacggcccg cttccgtctc
      661 caccgcggcg agcgcggcgg ccgcttcctc ggcagcgagc tcgtctgccg tattctcctc
      721 cgtggagaga agctcgtcgg catcgggcgc ggtctcgtac accgtgatgc ccatgtcgtt
      781 gatcatgcgg atgatgtctt cgatctgatc aggatcggag atatcgtcag gaagatggtc
      841 gttcacctct gaataggtga tatagccctg ctccttgccc ttggcaatga gctccttcag
      901 ccgggattgc tgctgttcaa tctgcgtgct catgacacct caaatagtga aatgcgttgg
      961 ggcagagagg ttccccgcat tacagcatcc ctgacttaat gctgtggtcg tgggttcccg
     1021 cccggattcg cggactgccg aaaatgagtt aacagccgaa tataccatat ataggggcgc
     1081 ttactgaaag ttaaacaatt gtccgacaaa aaagtcgctt gcgcaccgcc tggctaatcg
     1141 gtgccgctga ccttgtctcg agggccctgc tttaaagacc aatagtcctt caggcgctcc
     1201 gccgacggat cctggtgcat ctcggagatc agctgccgcc tggcagacgc ctgctgcatc
     1261 tccagatatc gggccacgcc ctcggtgaaa tcgccacgca ccgccgcgat gtcgatggcc
     1321 gacggctgcc gggcaagacc cgtcagctcg tcgtgaagat cgtgccccgt ccagcgcccg
     1381 agcagctccg gcgtctcggc atccgggtgc tcgtccagat aggtcacgag ttcggccagt
     1441 agatcgttcc ccgccagcgg ccgcagggcc cggcgggcct cttccgaaag ggctgagacc
     1501 acggacggtt cgcgcagcag atacccgacc aggcgtctcg ccaggggcgt caggctgaca
     1561 tcggcagccg tccggcgcgc cggccccctc gatggtcgcg cctgggcgct cggccggaag
     1621 cccgtcagcc cctccagccg ggagaccatg agctccttta ggatgcccgc gggcacccgg
     1681 tcgatgtgcg gcatggccag gctcgcgaac ctggccttgt catccacgct gccgagatcg
     1741 aggccttcct cgagccggcc gaaaagatac tccactgccg gcaccgcccg ggcggccagc
     1801 tcgagatatc cggcctttcc gcgggcgcga acgagagagt ccggatcctc gccgtccggc
     1861 agaaacatga acttcagccc acggccgtcg ctcaaggccg gcagggcgct ctccagcgcc
     1921 cgccacgccg cctggcgccc ggccgcatcg ccgtcgaagc agcagatcac ctcccccgtg
     1981 tagcggtaaa ggctctgaaa atgctcctcg gtagccgccg tgccgaggct ggccaccgcg
     2041 ttggatatgc ccgcctgggc cagcgccacc acgtccatgt agccttccac cacgatcagg
     2101 cggtcgatcc ggcgcagggc ctggcgcgcc tcgtagagac cgtagagctc ccggcccttg
     2161 tggaagacgg gggtctcggg gctgttgaga tacttcggcc ctccgccatc ggccatcacc
     2221 cgcccgccga agccgatgat gcggccccgc gtgtcgcgga tgggaaacat gatgcggcca
     2281 cgaaaccggt cataggcccg acccttctcg ccccgggtaa ggagcccggc ttccaggagc
     2341 ttggcttccc ccatgccggc aagcgcgctc gcggccccct gccactcgtc cggcgcgtac
     2401 ccgatcccga agtcccgcgc gatggtgccg gtgacccccc gggccttgag gtactcgatg
     2461 gcctcaggcg actggcgcag cgccttgcgg aagtaacgat ccgcggcggc gagcacgtcg
     2521 tagagatcgg tgttgtcccg cgccggccgc cccccttgct cccgcggaac ctcgacgcca
     2581 accagctggg ccagcgcttc caccgcttcc acgaactcca tgcggtcgtg ctccatgagg
     2641 aacgtcagcg ccgtgccgct ctcgccgcag ccgaagcagt gatagtactg cttctccggg
     2701 ctcacggtga acgagggcgt tttctcgttg tggaacgggc agagcgcctg atagtcgcgc
     2761 ccggcctttc tcagctgcag gcgcgcgtcg atcacatcca cgatgtccac ccggttcagg
     2821 aggtcattga tgaaggattg aggtatgcga ccggccatcg cagtcagatt agcaacgaat
     2881 tcttcgggag tctgcaatcg gctgcctgac gacgcgcttt aaatgtgata tgtctagaca
     2941 tacgaatcgc aggggacccg tcatgagaac caccgtgaga ctgccgggag agctgctgac
     3001 caaagccaaa gagttcgcac atgaacgcgg gacgacgctc acggcgctga tcgaggaggg
     3061 cctcataatg gttcttgaac gcgcgccgaa ggcgggaaag atgaaacctg ttcgagtacc
     3121 cgtctccaag tcgagtggcg gcctgcagcc aggagtggat ctgagcgatt cggccgctct
     3181 gctggatgcc atggagcaag gttgaagctc gccgacgtca acgtcctggt gggcgcgttc
     3241 cggaccgact catcgcatca cgagagatgc cgaagctggc tgaagagcac ggtcaacgcg
     3301 cccgccagct tcgggctcgc cgcgcaggtg ctgtccggcg tcatccgcgt tacgacgcac
     3361 ccgaaagtct tcgtaaaccc gagcccgatc gacgaatcaa ttcgtttcgc gaacttctta
     3421 ctcgggcgac cgaacgccat cgtcgtcagc ccaggcgagc gtcactggac gatcttcacg
     3481 gacctgtgcc gatctggcgg tattcaagga aatctggtgc ccgatgcctg gttcgcggct
     3541 cttgccatag agtcggggtg cgagtgggtg accctgaacg ggaacttcgc ccgtttcgag
     3601 ggcctcagct ggagcgcgcc ctgagcgccc tcagccctcc cggccgtaga tgtcgtcgaa
     3661 acggacgatg tcgtcctcgc cgaggtagga gcccacctga acctcgatga gctctaaggt
     3721 gagcttgccc ggattggcca gccggtgggt caccccgagc gggatgtaag tgctttggtt
     3781 ttccgccagg gtgatcgtct cgtcgccgcg ggtgacctcg gcggtccccc gcaccacgat
     3841 ccagtgctcg gcgcgatgat ggtgcatctg cagcgaaagg ctcgcgcccg gcttcacctt
     3901 gatccgcttg acctggtaac gctcgccggc acccacggac tcgtagctgc cccagggccg
     3961 gaacacctcg cggtgatgga cgtactcgtc gcggccggcc gccttgagct gctcgaccac
     4021 cgcgcgcacg tcctggaccc ggtccttgtc cgccactaaa acggcgtctg aggtttccac
     4081 caccaccagg tccgagacgc caatggtgcc aaccagccga tcgcgggccg agacgaaaga
     4141 gttctgcgta tcgacgctga tgacgtcgcc ctccaggcgg ttgccgtttc cgtcccgatc
     4201 cgacgcctcc cagatggcgc tccaggaacc cacgtcgctc cagtcgaagc cggccgggac
     4261 caccatggct cggtcggtct tctccatcac cgcatagtcg atggagtccg cggggcaatc
     4321 gaggaactcg ggccccggtc ggaaaaagtc gagatcttcg gccccgcgct cgaaggccag
     4381 cgacgccttc tccagcatca aagggctgtg ccggccgagc tcatccagat acgcccgggc
     4441 attgagcagg aacatgccgc tgttccagaa atagtcgccc gcggcgagat actcgttcgc
     4501 cgtcgccagg tcgggcttct ccacgaagga tcggacccgc acgacgccgg aacccaactc
     4561 cgcgccgggt gcttcgatgt agccgtatcc ggtctccggg ctggtcggca ccacgccgaa
     4621 cgtgaccagt gcgccttcac ccgcggcccg ggcggcgagc tcggcagcag acgcgaaccc
     4681 gtccaggtcg gtgatcagat gatccgacgg cagcacgagc agcaacggtg ccgaatcggg
     4741 ccccgcctcc tcgcaggcgg caagggcagc cagcgcgatg gccggcgccg tgttgcgccc
     4801 ctcgggctcc aagatgatgc gccgccagga ctggccgatg gcccggcact gctcggcgac
     4861 catgaagcgg tgttcttcgt tgcacacgat gatgggcgct tcgcccgtgg tacggtctgc
     4921 ccgcttgagc gtcgactgca gaagggagtc gtcgccgaga agggcgtgga actgcttggg
     4981 gaaaagctct ctggagagcg gccagagccg gctgccggtt cctccggcca gaagtacggg
     5041 aatcactttt tccatcgctc gctcgtcact ccgcacgacg gcattgccgc gccaagatac
     5101 cagatcctcg gattatcacc gtgggcggat ttgtaagtga ggccccggcg ctgccaacta
     5161 acctgctgac taaccggcta aaagcgcttt gacgcgaccg ctgaccgcgc ccatgtctgc
     5221 gcggccctga agccggtctt tcagcaggcc catgaccttg cccatgtcgc gcatggattc
     5281 cgcattggcc tcggcgatgg cctggtcgat ggcgctcgca agctccgcct cggagagctg
     5341 ctccggcatg aactcgccga tcaggtcgat ctcgaagctc tcctggtcgg ccagatcctt
     5401 gcgcccggcc tgggtgtact gctccagggc atcctgacgc tgtttgagca tgcggttgag
     5461 cacggccagc acttcggcgt cggtggaggc cgcacggcgc tcgtcgatct ccgcccgctt
     5521 gatgtccgca ttgaccaggc gcagcactgc caggcgctgc ttgtcgcgcg ccttcatggc
     5581 ggccgtggtg gccgcggtga tggcttgcct taactcggag atggggagat cggacacggc
     5641 gttcccctgt cgtgcggtgt cagaaggact tttcgaagcg ccgggcttcc cgctgcagct
     5701 tcttcagatg gcgcttgact gccgccgccg ccttgcgctt tcgaaccgac gtcggcttct
     5761 cgtaatactc tcgtctccgc acctcggaaa gcacgccggc cttctcgcag gaacgcttga
     5821 atcgcctgag agcgacatcg aacggctcat tctctttgac ttttacgctg ggcatgttgt
     5881 gcgcgaaatc ccctcgaacc tgacggttgt tgctgaaaag ggcgcggatt ttactggcgc
     5941 cggacggttt ttgcaaaacg ccggaatcgt taccatccgc ccatgctggt cctcggaatc
     6001 gaaacttcct gcgatgagac gggcgtcgcg ctctacgacg gtgtcgaggg gctgctggcg
     6061 gacgctctgc acagccaggt ggaagtgcac gccgcttacg gcggcgtggt gcccgagctg
     6121 gcctccagag accacatccg caagctggtt cctcttgtcc gcaagacgct ggacgattcc
     6181 ggacgctccc tcgcggacgt tgacggcgtc gcctacacgt ccggccccgg gctcatgggc
     6241 gcgttgctgg tgggcgcggg ttttgcgagg agcctggcct ggtcgctggg caaaccggcc
     6301 gtcgcggtgc atcacatgga ggcgcacctg ctggccccgc tgatgaatcc ggacacggcc
     6361 ccgcctatgc cgttcatcgc cctgctcgtt tccggaggac acacccagct ggtgctggtc
     6421 aacgcgatcg gcgattacga gctcctcggt gagtccgtgg acgacgccgc cggggaggcc
     6481 ttcgacaagg cggcgaagat gctggatctc ggctatcccg gaggtcccaa cctggcgcgc
     6541 gccgcggaag ggggcgatcc gcaccgattc acgttcccga gacccatgac cgaccgcccc
     6601 gggctcgact tcagcttcag cggattgaag acctacaccc tgaacacgac ccgcgacctg
     6661 gcccccatga gcgacgcaga ccgcgccgac gtcgcgcgcg cgttcgagga ggccgtggtg
     6721 gataccctct acatcaagtg ccgccgggcg gtggagcaga cgggcatcac ccatctggta
     6781 atggccggcg gcgtggcggc caacgcccgg ctgcgcagga agctcggcga ggacttgcgg
     6841 gccgccgtct actacccccc ggcgcggctg tgcaccgaca acggagcgat gatcgcctac
     6901 gccggctgcc tgcgccttca ggcgggcgag cgagcgcccc ttgccatcga gacccgtgcg
     6961 cgctggccca tcgtcgagct cggccgcccc ggcccgccgg cctgacccat ggacagcgtc
     7021 ttcgtcagga acctgaccgt ctccaccatc gtgggcgtct accccgaaga acgcacccgg
     7081 cctcaaaggg tgatcgtctc cttcgagctg tttgccgata cccgggcagc cgcccaaagc
     7141 gatgacctgg aagacgccct cgactacggc gaagcggcga gccgggtcgc ggagttcgtc
     7201 cgcggcagcc gcttcgagct catcgaagcg ctcgccgacg gcatcgcggc gctgctgctg
     7261 gaaggctatc ccgccgcgcg cgtggtggtg gaagtgctca agcccggggc ggtcacagac
     7321 gcggagacgg tgggcgtgaa aatcgagcgt tcgcgggcct gacctttcgc ccaacgaatc
     7381 aacacatcgg ccagaggcac gtctcgaccc ggcctacctg ccgacgctcg aacgctgggc
     7441 ctggcgaatg gcctgcgtgc gcgcctggtc gatggcctcg cccagagcgg ctccggtcag
     7501 ggagcctcca acgctggccg tcgtcacctc ctgacgaatc gaggccgcca gctcgagcag
     7561 cggctcggga ttgcgccccg cgcaggcggc cgaaacgcgc accgccatct ctggatcgtc
     7621 acccgccttg aatgccccca cggacttcag cgccgcgcac aggcttcccg cctcggcggt
     7681 ctcccaggca gcaagtacca ccccgtgaga ggcgatgtgg cccatggccc ggcggtgtcg
     7741 gcgcggcgcc cgaagccgtt cacatagcgt atctaccgcc tccggggcga gatgccagcc
     7801 aagggcggcg aaccgaccga gcgcgtcttc gattgcttcc gggaaggtga tctcgacttc
     7861 ggcgagctcg gcgaaccacg tcgacaagcc gcccacggat gccagaacgg tgaaaaagcg
     7921 caggggcgcc ttcgatccca gtgccttgac cagctcctgc cagacccgct ccgcggagag
     7981 ctccgccagc gcgccgctgc cggcgagctc ggccatgaga gcccgggtct cttcggcgac
     8041 ggtgaaatca tcgagctggg cggcgaaccg ggccacccga aatacccgga gcggatcctc
     8101 ggagaaagcg accgagacgt gcctgagcac tttcgcttcc aggtccgatc gaccgccgaa
     8161 gggatcgatc agggagccgt ccgacgcccg cgcgatggcg ttcacggtga ggtcgcggcg
     8221 ctccaggtcc tgctccagag tgacgtcggg cccggcgtga cactcgaaac ctttgtgccc
     8281 cggccccgtc ttgcgctcgg tgcgggccag cgcgtactcc tcgctggtgt ccgggtgcag
     8341 aaagaccggg aaatcacggc ccacctgccg aaagccgagt ttcagcatcg cctcggggga
     8401 ggcgcccacc accacccagt cgcgctcgcg taccgggcgg ccgagcagct catcgcggac
     8461 cgcgccgccg accagatata cgtccatggc tcattctgag aaagtcagcc cacaaagggg
     8521 ccggtgacct cgtaagtgat gccgcccgtc acccgaccgg aaaagccgcg ctgggagctg
     8581 aagtaaaggc gtgacatgtc gggcgtgaac gccaggccgg tgatttccga tccgccctgg
     8641 cgagtgacct gcagcagggc aaccggctcg ccctcccggg ggagaacgac gatctgcatg
     8701 tcatcgccgt cttcggccac cagcacgtcg ccggatgcat tggtttccac gttgtcgacg
     8761 ccgccaagta tgggcgttgc ggccgtggcc tgatcgtaga tcacctccag gcgatcctcg
     8821 ccgagatcga gagcccagac ccggttgtcg cccttggtcg cgagatagac cacaccgtcg
     8881 cagtagctca tcccctcgcc gccgtcgaag ccggtgcttt caggcacctg ccagcgggtg
     8941 ggcttgcggc ccgcttcggg gtccggaacg cgcagccaca ccaccgagcc
//
LOCUS       DBNHKV010000141         2612 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1438039, whole genome shotgun sequence.
ACCESSION   DBNHKV010000141 DBNHKV010000000
VERSION     DBNHKV010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1438039"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="AC16PP_04645"
     CDS             <1..366
                     /locus_tag="AC16PP_04645"
                     /inference="COORDINATES: protein motif:HMM:NF019948.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1722 domain-containing protein"
                     /protein_id="HIR6480946.1"
                     /translation="MAHSVPYYQAAGRLLSDLRGDLPGKAAEYFSLLMRGLSRPATRK
                     GHANVLSHLQGYFKRLLDGPSRQELEALIHSYRRGEQPLDAPLTLLKHHLRRHPQEYL
                     EYQVYLDPHPGFAGLRRLL"
     gene            475..1875
                     /locus_tag="AC16PP_04650"
     CDS             475..1875
                     /locus_tag="AC16PP_04650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6480947.1"
                     /translation="MSKDYDVIVVGSGAAGLAAAVAAADAGASVLLVEADKRVGGSSR
                     LSGGHFYAAGTSTQREAGVDDDTADAMFEHYMTLNQWLVEPSVVRRYCDLSAPTFEWL
                     KGQGVSFSPQAVYRSGVGSVARGHPPEGSGEAVVNILDARRSSLGVDVVLDARVDGLL
                     LDDAGRVHGIRIGDDEATCGAVVMATGGFGANPEMLAEYYPAAAANGDWSWYIGTEMA
                     RGDGIRLGQDAGAAIDGHNRGLLLVTPGFSRDLEVLLPGWLVLINEQGRRFTDETAPY
                     TVLAGLIQHQTGKVYAVFDEAARRGAKRNPASQAYWVDEVLEQKADAGRILRADSLDE
                     LAAAAGIHADGFKGTVARYNADCEKGQDTAFFKSPETGMRAIEEGPFYAAEVRPAIIC
                     WTGTGLRIDAEARVLRTDEHPIPGLFAAGELVGSLHGDRYIGGGGSFGPCIVFGKLAG
                     ENAASYTLTQAWAQDR"
     gene            complement(1847..>2612)
                     /locus_tag="AC16PP_04655"
     CDS             complement(1847..>2612)
                     /locus_tag="AC16PP_04655"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6480948.1"
                     /translation="QIELRNLAFAYDPAKPVLKDINLTINRGETVAVVGPTGGGKSTL
                     VNVICRFYEPTAGQVLIDGVDYRNRSLHWLQANLGMVLQNAHVFSGSIMENIRYGRLD
                     ASDEEVREAARLAGADEFVRALEKGYDTEAGEGGNRLSAGQKQLVSFARAILADPQIL
                     VMDEATSSVDTHTEQRIQRGMANVLAGRIAIVIAHRLSTIRNADRIIVVEEGRITESG
                     THAELMGRRGHYFELYKRQGLQESSATLSDLEPRPA"
BASE COUNT          414 a          852 c          893 g          453 t
ORIGIN      
        1 atggcgcaca gcgtcccgta ctatcaggcg gcgggcaggc tcctgagcga tctgcgcggc
       61 gatctgcccg gcaaggcggc cgagtatttc tccctgctca tgcggggcct gtcccgaccg
      121 gcgacccgca aagggcacgc caacgtgctc tcccatctgc agggatattt caagcgcctt
      181 ctggacgggc ccagccgcca ggagctcgaa gcgctgattc acagctaccg ccgtggcgag
      241 cagccgctgg acgctccgct caccctgttg aagcatcatc tgcgccgtca ccctcaggag
      301 tatctggagt atcaggtcta tctggatcct catccgggct ttgccggcct gcgtcgattg
      361 ctctgagcgt tctgcgccct ggggcccttg ccgttgacca accggtggcg cgtcgccctt
      421 aacggggtaa actcttcccg acgcgatggt gaacgcagcc aggggaggga caccatgagc
      481 aaagactacg atgtgatcgt cgtggggagc ggcgcagccg gtctcgccgc ggccgtcgct
      541 gctgcagacg ccggagccag cgtgcttctg gtcgaggcgg acaaacgggt gggcggatcg
      601 tcacgtctca gcggcgggca tttctatgcg gcgggcacca gcacccagcg ggaggccggg
      661 gtcgacgacg acacggccga tgccatgttc gagcactaca tgacgctgaa ccagtggctc
      721 gtcgagccct cggtggtccg ccgctactgc gatctgtccg cgcccacgtt cgagtggctc
      781 aagggtcagg gcgtcagctt cagcccccag gccgtctacc ggtccggggt gggcagcgtt
      841 gcccgtggcc acccgccgga aggatccgga gaggccgtcg tcaacatcct cgacgcccgt
      901 cgttcgagtc tgggcgtgga cgtggtgctg gacgcgagag tggacgggct gctcctcgat
      961 gacgccgggc gggtccacgg catcaggatc ggcgacgacg aggcgacctg cggggccgtg
     1021 gtgatggcca ccggggggtt cggtgccaac cctgagatgc tcgcggagta ctacccggct
     1081 gcggcggcca acggcgactg gtcgtggtac atcggaaccg agatggcccg gggcgacggc
     1141 atccggctcg gtcaggacgc cggtgccgcc atcgacggcc acaaccgcgg tctgctgctc
     1201 gtcacgcccg gcttcagccg cgatctggaa gttctgctcc cgggatggct ggtcctcatc
     1261 aacgagcagg gacgccgctt caccgacgag acggcgccct acacggtgct tgcggggctc
     1321 atccagcatc agacaggaaa ggtgtacgcg gtgttcgacg aagccgccag gcgcggggcc
     1381 aaacgcaacc cggcctccca ggcgtactgg gtggacgagg ttttagagca gaaggccgat
     1441 gcggggcgca tccttcgggc ggactccctc gacgagctcg ccgctgctgc gggcattcac
     1501 gcggatggct ttaagggcac cgtggcccgc tacaacgccg actgcgagaa gggccaggac
     1561 acggcgttct tcaaaagccc ggaaaccggc atgcgcgcca tcgaggaggg accgttctac
     1621 gctgcggaag ttcgtccggc catcatctgc tggacgggca cgggcctgcg catcgacgcg
     1681 gaggcccggg tgttgcgcac cgacgagcac ccgattcccg ggctcttcgc cgcaggcgag
     1741 ctggtgggct cgctgcacgg agaccgctac atcggcggtg gcggttcttt cggcccctgc
     1801 atcgtgttcg gcaagctggc tggtgagaac gccgcctctt acacgctcac gcaggcctgg
     1861 gctcaagatc gctgagggtg gcgctcgact cctgcaggcc ctgccgctta tagagctcga
     1921 aatagtggcc acgtcggccc atgagctcgg cgtgggtgcc cgactcggtg atgcggccct
     1981 cctcgaccac gatgatgcgg tcagcgttgc ggatagtgga cagccggtgg gcgatgacga
     2041 tggcgatgcg tcccgccagc acgttggcca tgccccgctg aatccgctgc tcggtgtgcg
     2101 tatccaccga tgaggtggcc tcgtccatga cgagaatctg cgggtccgcc agtatggcgc
     2161 gggcgaagga gacgagctgc ttctgtcccg ccgaaagacg attcccgccc tcaccggctt
     2221 ccgtgtcgta gcctttctcc agggcgcgga cgaattcgtc cgcgccggcc agccgggctg
     2281 cttcccgcac ctcctcgtcg ctcgcgtcga gccgtccgta gcggatgttc tccatgatcg
     2341 agccgctgaa aacgtgggcg ttctgcagaa ccattccgag gttggcctgc agccaatgga
     2401 ggctgcggtt ccggtagtcg acgccgtcga tgagcacctg gcctgcggtg ggctcgtaga
     2461 aacggcagat cacgttgacc agcgtgctct tgccgccgcc ggtcggcccg accacggcca
     2521 cggtctcgcc gcggttgatg gtgaggttga tgtccttcaa caccggtttc gccggatcat
     2581 aggcaaacgc taggttgcgc agctcgatct ga
//
LOCUS       DBNHKV010000142         6367 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1365784, whole genome shotgun sequence.
ACCESSION   DBNHKV010000142 DBNHKV010000000
VERSION     DBNHKV010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1365784"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="AC16PP_04660"
     CDS             <1..187
                     /locus_tag="AC16PP_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480949.1"
                     /translation="YTFAPSLPAVLSDFVFGSEMALGKSISLLAVVNYTISSLCLALG
                     LKHYRRALDRARAWASA"
     gene            complement(237..1583)
                     /locus_tag="AC16PP_04665"
     CDS             complement(237..1583)
                     /locus_tag="AC16PP_04665"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6480950.1"
                     /translation="MAETNIQSAPQESRAEKLDAGHPQFLRGDPITGDRYYSQDFARR
                     EWDRMWTRVWHIAGRESELPDAGDWIVHNFLRESIIATRQSDGSIRAFYNSCQHRGNR
                     LVWAGEGRGNFTCSYHGWEYGTDGVLVHAQDPEDFDGGSPCGRVSLVEVPCDTWGGFV
                     WYTMDPEAPSLAAFLDPIPELFANRDLDAAVRVVRRAFTVDANWKFASDNFNESYHLP
                     TVHPELATTTDEDYRNTIFEAYSTGHNRMIEQGQPSMRAPRPNEVEAPWDEILAAWDL
                     DPADFAARARDGRIALQQQRRKLGPARGYGHYAKYSDDELTDYFHHTLFPNVTLTATA
                     DGVHFFRTEPHPADPEKCTFEYWFFASPVEGQDEVVTICGPRPLQEAEPEQFEYGSGA
                     VIEDMMDSFLIQDLGVAVGQQQGFHSRGYRDAILSGQESRVRRFHEVLNDYLEGRR"
     gene            complement(1604..2605)
                     /locus_tag="AC16PP_04670"
     CDS             complement(1604..2605)
                     /locus_tag="AC16PP_04670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6480951.1"
                     /translation="MTVNTQVLIDSLPQGKLTADNYRIAESPVPEIGDGEVLVQTTAF
                     AITAGTRAGLQGSASYAGAPQTGVVMNGTGVGEVTESRSEDFAAGDRVLGPTGWQAYS
                     VHKPRALTLIPEGHDPVDYLGPLGVNGLTAYFGLFEVGRPAAGETVMISAAAGSVGHL
                     VGQMATTVDARVVGVCGSDAKGRILTDELGFDAVVNYREDGFRQALKEATPDGVDVYF
                     DNTGGDILGAALRRMNVAGRIACCGVVSQYDTDNPEPGPRGIPGLLINKRLDMRGFLV
                     FDFADRYDEARGNIAAWLESGKLKALVDEVDGVEAAPAAFVDLLAGGNVGTRVVRVT"
     gene            complement(2689..3897)
                     /locus_tag="AC16PP_04675"
     CDS             complement(2689..3897)
                     /locus_tag="AC16PP_04675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spinster family MFS transporter"
                     /protein_id="HIR6480952.1"
                     /translation="MSGARSHWSVGGRNYVLVLLTATAAFNFVDRQILGVLLEPIGQE
                     FDLPDSLLGLLSGIAFAVFYAVLGIPIAALADRLNRRNIIVVCIALFSTMTALCGAVV
                     NFWQLFLARIGVGVGEAGTMPASQSMLSDLFPVQERPWAMGLLATGGNIGLMLGLLIG
                     GWVNEWFGWRAAFVIAAVPGALIAAVIYFTVPEPVRRHAAAARSGWMREAFTGMRLVG
                     SIRSVRRFAMGGTLYGLSAYGIHTWMPVYYIRYHGLSTGEAGTVISIIVGVLGGIGAF
                     AGGVFCAKLSQRDLRWNGWLPALAIFTSVPLLIAMLITDNTWLAIGLFVLPGILSSVY
                     AGPTWSIIQELVPAQRRAMAASVYMLFFNLIGLGLGPLTVGVLSDLYIPAIGEASLRW
                     AMVSVLLVSL"
     gene            complement(3929..4537)
                     /locus_tag="AC16PP_04680"
     CDS             complement(3929..4537)
                     /locus_tag="AC16PP_04680"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6480953.1"
                     /translation="MLILHHAPNSRAGRIVWLLEELGLDYELNRMDFHPKDLKSEAHR
                     ERHPLGRVPVLEDGEIMLYESGAIVEYIVARHSDGALKPSVDSPLFPEYLQWFHYCEG
                     MVMPPVNTIVVQTLLLPPERRNEEALGQAQRLLAKAVAPVDEALAGREYLIGDFSAAD
                     IMLGHALYMSRRLGAVSEEMTNLNAYIDRIEARPAFQKGIQA"
     gene            4685..6319
                     /locus_tag="AC16PP_04685"
     CDS             4685..6319
                     /locus_tag="AC16PP_04685"
                     /inference="COORDINATES: protein motif:HMM:NF025114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6480954.1"
                     /translation="MNDEPDVLVVGAGFAGMYAVHRLGELGLTVKVLEAGGDVGGTWY
                     WNRYPGARCDVPSLEYSYGFSPELEQEWDWPEVFSSQEDILAYANHVADRFDLRRHMR
                     FDTRIVAVEYREDENVWRLTSEAGEVFEAPFCVMATGCLSVPNTPRLPGADSFAGLVL
                     HTGRWPHEAVDLAGKRVGIIGTGSSGVQAIPELARQSGHLVVFQRTPVYTVPANRKAM
                     RPDVQREFREHYRSIRGMQQRNAGGVSGYRPLRGGRAGPAVPGERALRTGILDLDPDQ
                     RRRIVAERGLETLLNFPDVYTDPEANEVANALYRDAVRAEVRDPETAEKLLPKDYGLG
                     CKRQVLDRDFFETFNRDDVTLVDLRETPMETLNPDGIRTSHGQYDLDVLIYATGFDAM
                     TGALTRANVRGRDGQALADKWRYGPVAYLGLQMRGFPNLFTVTGPGSPSVLCNMLVAI
                     EQHVNWIGECITWLRETNRTRIEPLAESETEWVAHVNEVAEGTMYTAPSCNSWYLGAN
                     IPGKPRIFMPYVGGYPRYRERCEREAASGYQGFESG"
BASE COUNT         1106 a         2059 c         2145 g         1057 t
ORIGIN      
        1 gtacacgttc gcgccgtcat tgccggccgt gctgagcgat ttcgtgttcg gcagcgagat
       61 ggcgctgggt aagtccatct cgctgcttgc cgtcgtgaac tacacgatct ccagcctctg
      121 cctggccctg ggtctgaagc actatcgacg cgctttagac cgcgcgcggg cgtgggcgag
      181 cgcctagcgc tccgacgaca tctcaagcag gccgatttcc acgcagctgg cggatgctaa
      241 cggcgacctt ccagatagtc gtttaaaacc tcgtggaagc gccgcacccg gctctcctga
      301 ccgcttaaga tcgcgtcgcg atagccgcgg gaatgaaacc cctgctgctg gccgaccgcc
      361 accccgagat cctggatcag aaacgaatcc atcatgtcct cgatgaccgc accggagccg
      421 tattcgaact gttcgggctc ggcctcctgc agtggacggg ggccgcagat cgtgaccact
      481 tcgtcctgtc cctcgacggg cgatgcgaaa aaccagtatt cgaacgtgca tttctcagga
      541 tcggcgggat gaggctccgt acgaaaaaag tgcacgccgt ccgccgttgc cgtgagcgtc
      601 acgttgggga acagggtgtg atgaaaatag tcggtgagct cgtcgtccga gtacttcgcg
      661 tagtgtccgt agccgcgcgc ggggccgagc ttgcgccgtt gctgctggag cgcgatgcgc
      721 ccatccctgg cacgcgcggc aaaatcggcg ggatccaggt cccacgcggc caggatctca
      781 tcccacgggg cctccacctc gttggggcgt ggcgcgcgca tggaaggctg gccctgctcg
      841 atcatccggt tgtggccggt ggagtaggct tcgaagatcg tgttgcggta gtcctcgtcc
      901 gtcgtcgtcg cgagctcggg gtgcaccgtg ggcaggtggt aggactcgtt gaagttgtcg
      961 gaggcaaatt tccagttggc gtccacggtg aaggcgcgcc gcaccacgcg aacggcggcg
     1021 tcgagatcgc gattggcgaa cagctccggg atggggtcta aaaacgccgc gagtgagggc
     1081 gcctccggat ccatggtgta ccagacgaac ccgccccagg tgtcgcaggg cacctcgacc
     1141 agggagacgc ggccgcacgg gctgccgccg tcgaagtctt cggggtcctg cgcgtgcacg
     1201 agcacgccgt cggtaccgta ctcccagccg tgatacgagc aggtgaagtt gccgcgaccc
     1261 tcgcctgccc acaccagccg gttaccacgg tgctggcagc tgttgtagaa cgcgcggatc
     1321 gatccgtcgc tctgacgggt cgcgatgatg gactcgcgca gaaaattgtg gacgatccag
     1381 tcgccggcgt cgggcagctc cgattcccgc ccggcgatgt gccacacacg cgtccacatt
     1441 cggtcccact cacggcgggc aaagtcctgg ctgtagtagc gatcgccggt gatgggatct
     1501 ccgcgcagaa attgcgggtg tccggcgtcc agcttttctg cgcggctctc ctgcggagcc
     1561 gactgaatgt tggtttcagc cattccaggt tcctcctcga cgttcaggtc acgcggacca
     1621 cgcgcgtgcc cacgttgccc ccggctaaca gatcgacgaa cgcggcgggc gcggcctcga
     1681 ccccgtccac ttcgtctacc agggccttca gtttgcccga ctcgagccag gctgcaatat
     1741 tcccgcgcgc ctcgtcgtag cggtcggcaa aatcgaaaac gagaaagccg cgcatgtcca
     1801 ggcgcttgtt gatcagcaac cccgggatcc cgcgcgggcc gggttcggga ttgtcggtgt
     1861 cgtactggga gaccacgccg cagcaggcga tgcggccggc cacgttcatg cgccgcaggg
     1921 cagcaccgag gatgtcgcca cccgtgttgt cgaagtagac gtccacaccg tcgggtgtcg
     1981 cttccttgag cgcctggcgg aagccgtctt cccggtagtt gaccacggcg tcgaagccga
     2041 gctcatccgt gagaatgcgg cccttggcgt cgctcccgca aacgcccacg acccgcgcgt
     2101 ccaccgttgt ggccatctgc ccgaccaggt ggccgacgct gccggcggcg gccgagatca
     2161 tcaccgtttc cccggctgcc gggcggccca cctcgaagag cccgaagtag gcggtgagcc
     2221 cgttgacgcc cagaggcccc agatagtcaa cgggatcgtg gccctcgggg atgagcgtca
     2281 gcgcgcgcgg tttgtgaacg gagtacgcct gccagccggt gggcccgagc acccgatcgc
     2341 ccgcggcgaa gtcttcagag cggctttccg tcacctcgcc cacgccggtg ccgttcatga
     2401 cgaccccggt ctgcggcgcg ccggcatagc tggcgcttcc ctggagcccc gcccgggtgc
     2461 cggccgtgat ggcgaaggcc gtggtctgca cgagcacctc gccgtcgccg atctcgggca
     2521 cggggctctc ggcgatccga tagttgtcag cggtgagctt cccctgcggc agcgagtcga
     2581 tgagcacctg agtattgacg gtcatgtttg ggcacctccg gcacgcgagc gtagcacgtc
     2641 cagatcgccg atgagggacc tcgctgccag cacgtaggcg gtgatgcctc acaggctcac
     2701 gagcagcacg ctcaccatgg cccagcgcag cgaagcctcg ccaatggcgg gaatgtacag
     2761 gtcgctgaga acccccaccg tcagcggccc caggccaagg ccgatgaggt tgaagaaaag
     2821 catgtaaacc gacgccgcca tggcccgccg ctgcgcgggc accagttcct gaatgatgga
     2881 ccaggtgggc ccggcataga cgctggagag gatcccgggg agcacgaaca ggccgatggc
     2941 gagccaggta ttgtcggtga tgagcatggc gatcaggagc ggcacgctgg tgaagatcgc
     3001 cagggccggc agccagccgt tccagcgcag atcgcgctgg ctgagcttcg cgcagaagac
     3061 gccgccggcg aaagcgccga tgccgccgag cacgccgacg atgatggaga tcaccgttcc
     3121 cgcttcgccg gtggaaaggc cgtggtagcg gatgtagtaa acgggcatcc aggtgtggat
     3181 gccgtaggcc gaaagaccgt agagcgtgcc tcccatggcg aatcggcgga ccgagcggat
     3241 actgcccacc agccgcatcc cggtgaaggc ctcgcgcatc cagccggacc gcgcagccgc
     3301 cgcgtgcctg cggacgggct ccgggacggt gaagtagatg actgcggcga tgagcgcgcc
     3361 gggtaccgcc gcgatcacga aggcggcccg ccagccgaac cactcgttca cccagccgcc
     3421 gatgagcagg ccgagcatca gaccgatgtt gcctcccgtg gccagcagtc ccatggccca
     3481 gggtcgctcc tggaccggaa agagatcgga gagcatcgat tggctggccg gcatggtgcc
     3541 ggcttcgccc acccccacgc cgatgcgcgc gaggaacagc tgccagaagt tcaccaccgc
     3601 cccgcacaga gcggtcatgg tgctgaagag cgcgatgcac accacgatga tgttgcggcg
     3661 gttgagacga tcggcgaggg cagcgatggg aatgccgagc accgcgtaga aaacggcaaa
     3721 ggcgatgccc gaaagcaggc ccagcaggct gtcgggcagg tcgaactcct gacctatggg
     3781 ctcgagcagc accccgagga tctgtcgatc gacgaagttg aaggcggcgg ttgccgtgag
     3841 cagcaccagc acgtagttgc ggccgccgac ggaccagtgt gatcgcgcgc cgctcatttt
     3901 ccgctccggg ccctgtccgc ccgccggatc aggcctggat gcccttctgg aacgcgggcc
     3961 gggcttcgat gcggtcgatg taggcgttga ggttggtcat ctcctcggag acggcaccca
     4021 ggcgccggga catgtaaagg gcgtgaccca gcatgatgtc cgcggcggaa aagtcgccga
     4081 tgaggtattc gcggcccgcc agggcctcgt caacgggtgc caccgccttg gccagcagtc
     4141 gctgggcctg gccgagggcc tcctcgttgc gccgctcggg aggcagcagc agcgtctgca
     4201 cgacgatggt gttcaccggc ggcatgacca tgccctcgca gtagtgaaac cattgcaggt
     4261 actcggggaa cagcggcgag tccactgagg gcttgagagc gccgtcgctg tgccgcgcga
     4321 cgatgtactc cacgatggcg cccgactcgt agagcatgat ctccccgtcc tctaaaacgg
     4381 gcacgcggcc gagcgggtgg cgttcgcggt gggcctcgga cttgaggtcc ttgggatgga
     4441 aatccatgcg gttgagctcg tagtccagcc caagctcctc gagaagccag acgatgcgtc
     4501 cggcccgtga attgggcgcg tggtgcagta tcagcactga aatctctccc tccagacgac
     4561 gtcggcagtg taacccgaca ggcatggaat atcgctgcga gttccctgca gggccggccg
     4621 gtgacgctga gcatcggctg cccttagaat caggtcatgt caaagagggg aatccggcgc
     4681 ccggatgaac gacgaacctg acgtgctggt ggtgggtgcg ggttttgcgg ggatgtacgc
     4741 cgtgcatcgc ttaggcgagc tcggcctcac ggtgaaggtg ctggaggcgg gtggagacgt
     4801 cggcggcacg tggtactgga accgctaccc cggcgcccgc tgcgacgttc cgagcctgga
     4861 atactcctac ggcttttccc cggagctgga gcaggagtgg gactggcccg aggtattttc
     4921 ctcacaggag gacattctcg cctacgcgaa ccacgtggcc gaccggttcg atctccggcg
     4981 ccacatgcgg ttcgatacgc ggatcgtcgc cgtcgagtac cgggaggatg agaacgtctg
     5041 gcgcctgacc agcgaggcgg gcgaggtctt cgaagcgccg ttctgcgtca tggccaccgg
     5101 ttgcctgtcc gtccccaaca cgccccgcct gccgggtgcc gactccttcg ccgggctcgt
     5161 gctccatacc ggacgctggc cccacgaagc cgtggatctc gcgggcaagc gggtgggcat
     5221 catcggcacc ggatcctccg gcgttcaggc cattcccgag ctggctcgtc agtcgggaca
     5281 cctggtggtg tttcagcgca cgcccgtcta cacggtcccg gcgaaccgca aggcgatgcg
     5341 gcccgacgtg cagcgcgagt tccgtgagca ctatcgcagc atccgcggca tgcagcagcg
     5401 caatgccggt ggggtttccg gatatcggcc gctcagagga gggcgggctg gtcccgcggt
     5461 gcccggcgag cgcgccttgc gcacgggcat cctcgatctc gaccccgatc aacgccgtcg
     5521 gatcgttgcc gagcgaggtc tggaaacgct gctcaacttc cccgacgtct acacggaccc
     5581 ggaggccaac gaggttgcca acgcccttta ccgggacgcg gtccgggcgg aggtgcggga
     5641 tccggagacc gcggagaagc tgctgccgaa agactatggg ctgggatgca agcgccaggt
     5701 gctggaccgc gactttttcg agaccttcaa ccgcgacgac gtcacgctgg tcgacctgcg
     5761 cgagacaccc atggaaacgc tcaatcccga cgggatcagg accagccacg gtcagtacga
     5821 tcttgacgtg ctgatctacg ccaccggctt cgatgccatg acgggcgcgc tgaccagggc
     5881 gaacgttcgc ggccgcgatg gccaggccct ggcagacaag tggcgatacg gtccggttgc
     5941 gtacctgggt ctgcagatgc gcgggtttcc gaacctgttc accgttacgg ggccgggcag
     6001 cccttcggtg ctctgcaaca tgctggtggc catcgagcag cacgtgaact ggatcggcga
     6061 gtgcatcacg tggctgcgcg agaccaaccg cacgcgcatc gagcctctcg ccgaatcgga
     6121 aaccgagtgg gttgcccacg tcaacgaagt ggccgaagga accatgtaca ccgcgcccag
     6181 ctgcaattcc tggtacctcg gcgcaaacat accgggcaag ccgcgcatct tcatgcccta
     6241 cgtcggcggg tacccgcgct accgggaacg ctgcgagcgc gaggcggcgt cgggctatca
     6301 ggggttcgag tcgggctgaa cggctcccgg gccgatcggt ctcgcgggcg tctttcgatg
     6361 ctatgct
//
LOCUS       DBNHKV010000143         6573 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_373678, whole genome shotgun sequence.
ACCESSION   DBNHKV010000143 DBNHKV010000000
VERSION     DBNHKV010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_373678"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1518
                     /locus_tag="AC16PP_04690"
     CDS             <1..1518
                     /locus_tag="AC16PP_04690"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6480955.1"
                     /translation="EPIAQADLPPDAAPADPYGRDTPRGSMEGFLESAGEGDFERAAR
                     YLDLRNLPNALNRYSPETLARGLNLVLERGTWLDLTRLSNSPEGDLGDGLPTYRDPLD
                     TLSTSRGDAELWLQHVPSDVHEGMLIWKVSNRSVARLPELYDEFRFSPPLEYLRTTLP
                     DVRLLGVQLFKWVWSLAVGLAVWVILFGTSRLTTRGAIRRGTRWGAPLRYFLSVPVSI
                     SASLLLAGAVLRSLGLGLEAQRISEMQTGNLILIAWVFFTGINFARDLYIGKLQSQGR
                     EGTGALIRPLAAALKSVLGIIFVITWLDNAGYNIGALLAGLGIGGVAVALVLQKPLED
                     VFAAFTLFTQQPIRIGDFGRFGELTGTIEEISLRTTRIRTLENTVVAVPNAKFAAEPI
                     ENISARRRILYRPYVRLEFGTTQEQVENILQGVGKLLAGHPNVLEEGRRVRLLRFGEI
                     GIEIEGFAYIDTTVYVDYLEIAEELNLAIMNIVESAGARFAQLSGDSVKQMVATG"
     gene            1650..2627
                     /locus_tag="AC16PP_04695"
     CDS             1650..2627
                     /locus_tag="AC16PP_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6480956.1"
                     /translation="MPYEQIQALNERMQRSIIGQEAVVERLIIGLLANGNLLVEGLPG
                     LAKTRAIKSLAKNLEADFSRIQFTPDLLPSDVTGTEVYYQQEGRSEFRFEPGPIFANI
                     VLGDEINRAPAKVQAAMLEAMEERQVTVAGTTHPMPELFMVMATQNPVEQEGTYPLPE
                     AQMDRFLMHVNIEYPPVEDEVLIMDLVRAEEGATGSDGGSGSEEARIPQQAIFDARAE
                     IAAIHLSDAIKRYMADLVYATRTPQRYSDDLDRWIDVGASPRGSLALDKCGRAHAWLN
                     RRDFVDPGDVRAIVHDCLRHRLMLSYEAKGEGVTSDKVIDVLLEKVALP"
     gene            2635..3576
                     /locus_tag="AC16PP_04700"
     CDS             2635..3576
                     /locus_tag="AC16PP_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6480957.1"
                     /translation="MSVDPRIHVTLQHLISMQGAAGGITFLPRQASASVLNGRHASRL
                     RGRGLNFEEMRGYLPGDDVRSIDWKATARTGQPHVRVFTEERDRPALLVVDQRMSMFF
                     GSRLNMKSVTAAEAAAIAAFRILDAGDRVGGVVFGDDEHVEFVPRRSQRTVYGLLEAV
                     TEMNQGLHADKTVDARPGSLNDILKSVARIAHHDHLVLIFSDFDGIDEYTHRHLNGIA
                     QHNDIIAMLVYDPMAEGLEPGQRAVIGDSRMQAEVDLGSEATRTAVSGFSRQRLERIY
                     QWQTEINLSVLPLSAGEPTLTQIRRLTGGIAPKRKVR"
     gene            3573..4055
                     /locus_tag="AC16PP_04705"
     CDS             3573..4055
                     /locus_tag="AC16PP_04705"
                     /inference="COORDINATES: protein motif:HMM:NF025674.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HIR6480958.1"
                     /translation="MSAYDDLNLPQLLELMHELHLPEPISMVPQSPGWWVLAGWLAAI
                     ALMTAAHLAGRRRRNRYRREAQSSLDGIAARAADDPSGSAQEIAALLKRTALAAYPRA
                     TVASLYGADWAAFLCTTAGNDPEIAAAADGLAGSAYRADADGRALVSPAGRWIRLHRA
                     "
     gene            4048..5034
                     /locus_tag="AC16PP_04710"
     CDS             4048..5034
                     /locus_tag="AC16PP_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6480959.1"
                     /translation="MLDLASPWALLLTPLPLVIWWLLPPQREAVSALRVPFFDRIVAS
                     AGSQARAGAVIMRRRPLQMLVAATVWLLVVFGLARPEWVGEPIVRTEAARDIMLAIDL
                     SGSMDYRDFPDGDGRNIGRFEAVQKVVDRFIANRESDRVGMIVFGTKAYLQIPFTRDL
                     ESARALVKLMEVGMAGPKTALGDAIGLAIRTFESSEVDDRLLILLTDGNDTGSAMTPV
                     NAAEIAKLNDVEVYTIGIGDTEASGEDRVDFEALTEIAERTGGAFFNAEDASALTAIY
                     QRIDETAVADVRTQSWRPRASLVHWPAGAAALLIILAYAALLIGARRRNVPA"
     gene            5031..6017
                     /locus_tag="AC16PP_04715"
     CDS             5031..6017
                     /locus_tag="AC16PP_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024909.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR6480960.1"
                     /translation="MMELLHFIRPLWLAALPAIVCVWWLVRRRDALQASAGRFLAPHL
                     SEALTINRNARRGLRAVDGVAVTAILLALATAGPTWSRQANPWYAETAPVVVAVEVSD
                     SMRANDLQPTRLDRARFKILDLIAARTGSRTALIAYAGSAHTVMPPTTDAEVLRPFLE
                     SLDPAIMPTGGANAAAVLPLARSLLGEAAATGTLLFVNDGFDPVDVPALAAFARDPGN
                     PPMAALVLGTEAGGVALMPDGSPVQSEGGDRLDTRVDASLLRRAESEGGVTIVRTGPG
                     DADVRRLLRVVESNLRQADDPDATWRDQGWWLLWPAALLSLLWFRRGWTMQW"
     gene            6011..>6573
                     /locus_tag="AC16PP_04720"
     CDS             6011..>6573
                     /locus_tag="AC16PP_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6480961.1"
                     /translation="MVARLVIITLAAAGILGVLTWQRGDFTGLWLTPDQQALKAYERR
                     EFRDAFDAFEDPAWKGVAAYRSGLYGESADAFGRIDSARGFYNRGNAFMKGREYGKAI
                     TAYEQAVADDPDWLEARENLALARHVLDYIERVREQSDTGDDTELGADDYVFDNTRKK
                     GVEIEITQESTIELESAEKWMRSVDTQT"
BASE COUNT         1080 a         2285 c         2180 g         1028 t
ORIGIN      
        1 gaaccgatcg cccaggccga tctgcccccg gacgccgccc ctgccgaccc gtacgggcgc
       61 gacaccccga ggggatcgat ggaaggcttc ctggaatccg ccggcgaggg cgacttcgag
      121 cgtgcggcca gatatctgga tctgcgaaac ctgccgaatg ctctgaatcg gtactcaccg
      181 gaaacgctcg cccgagggct caatctggtg ctggagcggg gaacctggct ggacctgacc
      241 cggctcagca acagcccgga aggcgacctc ggcgacggcc tgccaaccta tcgggatccc
      301 ctggacaccc tgtcgaccag tcgcggcgac gccgagcttt ggctgcagca cgtcccgtcg
      361 gatgtgcacg agggcatgct gatctggaag gtatcgaatc gcagcgtggc caggctgccg
      421 gagctctacg acgagttccg cttctctccg cccctggagt acctgcgcac cacgctgccc
      481 gacgtgcgcc tgctgggcgt gcagctgttc aagtgggtct ggagcctggc ggtggggctg
      541 gccgtctggg tgatcctgtt cggcacgtcg cgcctgacga cacggggggc catccggcga
      601 gggacacgtt ggggagcgcc tttgcgctac ttcctgtcgg tccccgtctc catatccgca
      661 agcctgctcc tggccggggc cgtgctccgc tctctcgggc tggggctcga ggcccagcgc
      721 atctcggaaa tgcagaccgg caatctcatc ctgattgcct gggtcttctt caccggcatc
      781 aacttcgctc gggacctgta catcggcaag ctgcagagcc agggccgcga gggcacgggc
      841 gcgctgatcc ggccgctggc cgccgcactc aaatcggtgc tggggatcat cttcgtcatc
      901 acctggctcg acaacgccgg ctacaacatc ggcgccctcc tggccggcct cggcatcggc
      961 ggcgtagccg tagcactcgt gcttcagaaa cccctggagg acgtgttcgc agccttcacg
     1021 ctgttcaccc agcaacccat ccggatcggc gacttcggcc gcttcggaga gctcaccggc
     1081 accatcgagg agatcagcct gcgaaccacg cggataagga cactggagaa caccgtggtc
     1141 gccgtgccca atgcgaagtt cgccgcggag cccatcgaga acatctcggc tcgccgccgg
     1201 atcctgtacc ggccttacgt ccggctggag ttcggtacca cccaggagca ggtggagaac
     1261 atcctgcagg gagtcgggaa gttgctcgcg ggtcatccga acgtgctcga ggagggccgc
     1321 cgggtaaggc tgctgcgatt tggcgagatc ggcatcgaga tcgaaggctt tgcctacatc
     1381 gacaccaccg tgtacgtgga ctatctggag atcgccgagg agttgaacct cgccatcatg
     1441 aacatcgtgg agagcgcggg cgcccgattc gctcagctct ccggcgacag cgtcaagcag
     1501 atggtggcga ccggctgatc cgtgtgcaga tgggggtcgc agccgcgctg tcgacacgtc
     1561 cgcgccgccg tcggcatact gctgcctgac accaccggga tcattcgtcg cgccgctgac
     1621 ccggacgcgg ccgggaagca cgacacacca tgccctacga acagattcaa gcgctcaacg
     1681 agcgcatgca gcgctccatc atcggccagg aagccgtggt ggagcggctc atcatcggcc
     1741 tgctcgccaa cggcaacctg ctcgtcgagg gccttcccgg cctcgcgaag acccgggcga
     1801 tcaagtccct ggccaagaac ctggaggccg acttcagccg catccagttc accccggacc
     1861 tgctgccctc ggacgtgaca ggcacggagg tctactacca gcaggagggg cgcagcgagt
     1921 tccggttcga gcccggcccg attttcgcca acatcgtcct gggcgacgag atcaaccggg
     1981 cgcccgcaaa ggtccaggcc gccatgctgg aagcgatgga ggaacgccag gtcaccgtgg
     2041 cggggacgac ccatcccatg cccgagctct tcatggtcat ggccacccag aacccggtgg
     2101 agcaggaagg aacctacccg ctgccagagg cgcagatgga ccgctttctg atgcacgtca
     2161 acatcgagta cccgccggtg gaggacgagg tgctcatcat ggatctggtt cgggcggaag
     2221 aaggcgccac cggcagcgat ggcggctccg gatcggagga ggcgcgcatc ccccagcagg
     2281 cgatcttcga cgcccgggcg gagatcgcgg cgattcacct ctccgacgcc atcaagcggt
     2341 acatggcgga tctggtctac gccacccgca ccccgcagcg ctactcggac gacctcgacc
     2401 gctggatcga cgtcggtgcg agcccccggg ggtccctggc gctggacaag tgcgggcgcg
     2461 cccacgcgtg gctgaatcgt cgggatttcg tcgaccccgg cgacgtccga gcgatcgttc
     2521 acgactgcct gcgccaccgg ctgatgctct cctacgaagc caagggcgag ggcgtcacct
     2581 cagacaaggt gatcgacgtg ctgctggaga aggtggccct gccctgactc gagcatgagc
     2641 gtcgatcccc gcatccatgt caccctgcag cacctcatct ccatgcaggg agcggccggc
     2701 ggcatcacgt tcctgccgcg acaggcttcg gccagcgtcc tcaacggccg gcacgcttcc
     2761 cgtctgcggg gccgcggcct gaacttcgag gagatgcgcg gctaccttcc cggcgacgat
     2821 gtccgctcca tcgactggaa ggccaccgcg cgcaccggcc agccccatgt tcgggtcttt
     2881 accgaggagc gtgatcgacc cgcgctgctg gtcgtggatc agcggatgtc catgttcttc
     2941 ggctcccggc tcaacatgaa atccgtcacc gccgccgaag ccgccgccat cgccgccttc
     3001 cgcatactgg atgccggcga ccgggtcggc ggcgtggtgt tcggcgacga cgagcacgtg
     3061 gagttcgtgc ccaggcgcag ccagcgcacg gtctacgggc tgctggaagc cgtcaccgag
     3121 atgaaccagg gtctgcacgc cgacaagacc gtggacgccc gacccggcag cctgaacgac
     3181 atcctcaaga gcgtggctcg catcgcccac cacgatcatc tggttctcat cttcagcgac
     3241 ttcgacggca tcgacgagta cacccaccgg cacttgaacg gtatcgcgca gcacaacgac
     3301 atcatcgcga tgctcgtgta cgaccccatg gccgaggggc tcgagccggg ccagcgggcc
     3361 gtcatcgggg acagccgcat gcaggcggag gtggacctgg gatcggaggc gacgcgcacc
     3421 gccgtcagcg ggttcagccg ccagcgcctc gagcgcatct accagtggca gaccgagatc
     3481 aacctttcgg tgctcccgtt gagcgcgggt gagcccacgc tgacccagat ccgccgactg
     3541 acgggcggca tcgcgccaaa gcgcaaagta cgatgagcgc ttacgacgac ttgaacctgc
     3601 cccagctcct ggagctcatg cacgagctgc acttgcccga gccgatctcc atggtgcccc
     3661 agagccccgg ctggtgggtg ctcgccgggt ggctcgcggc cattgcgctg atgaccgccg
     3721 ctcaccttgc cgggcgccgc cggcgcaacc gctatcggcg cgaggctcaa tcctcgctgg
     3781 acggcatcgc cgcccgggcg gcggacgatc cgtccggaag cgcccaggag atcgccgccc
     3841 tgctgaaacg aacggcgctg gccgcttacc cgcgggcgac ggtcgcgtcc ctttacggag
     3901 ccgattgggc ggcctttctc tgcacaacgg ccggcaacga tccggagatc gccgctgccg
     3961 cagacgggct cgccggcagc gcctaccgcg ctgacgcgga cggccgtgcg ctcgtcagcc
     4021 cggccggtcg ctggatcagg ctgcatcgtg cttgacctgg cctccccctg ggcgctcctg
     4081 ctcacaccgc tgcccctggt gatctggtgg ctgctgccgc cccagcggga agcggtgagc
     4141 gcgctgcgcg tgcccttctt cgatcggatc gtagccagcg ccggttccca ggcgcgggcc
     4201 ggcgcggtga tcatgcgccg ccggcctctg cagatgctcg tggcggccac cgtgtggctg
     4261 ctggtcgttt tcggcctcgc gcggccggag tgggtgggcg agcccatcgt gcgcaccgag
     4321 gcggcgagag acatcatgct ggccatcgac ctgtccggtt ccatggacta ccgggacttt
     4381 cccgacggcg acgggcgcaa catcggccgg ttcgaggccg tgcagaaggt ggtggaccgc
     4441 ttcatcgcca accgcgagag cgatcgggtc ggcatgatcg tgttcggcac caaggcctat
     4501 ctgcagatac cgttcacgcg cgacctggaa tcggcccgcg ccctcgtgaa gctcatggag
     4561 gtcggcatgg ccggtcccaa gacggccctg ggagacgcca tcgggctggc catccgcacc
     4621 ttcgagtcga gcgaggtcga cgaccgtctg ctcatactgc tgaccgacgg caacgacacc
     4681 ggcagcgcca tgacgcccgt caacgccgcg gagatcgcga agctgaacga cgtggaggtc
     4741 tacaccatcg ggatcgggga caccgaagca tcaggcgagg accgggtgga cttcgaggcg
     4801 ttgacagaga tcgccgagcg cacgggcggc gccttcttca acgcggagga cgcctctgcg
     4861 ctgaccgcca tttaccagcg catcgacgaa accgcggtgg ccgacgttcg cacccagtcc
     4921 tggcggcccc gggcatccct cgtgcactgg ccggcgggcg cggcggcgct gctgatcatc
     4981 cttgcctacg ctgcgctcct gatcggtgcc cggcgaagga acgtgcccgc atgatggagc
     5041 tcctgcactt catccggccc ctgtggctcg ctgctctgcc agccatcgtg tgcgtgtggt
     5101 ggctggttcg gcggcgcgat gcgctgcagg cgagcgccgg ccggttcttg gcgccgcact
     5161 tgagcgaggc gctgaccatc aaccgcaatg ccaggcgcgg cttacgcgcc gtggacggcg
     5221 tcgcggtcac ggccatcctg ctcgccctgg ccacggcggg gcccacctgg agccgacagg
     5281 ccaacccctg gtatgcggag accgcgccgg tggtggtggc cgtggaggtg tccgacagca
     5341 tgcgcgccaa cgacctgcag ccaacccgcc tggatcgggc ccggttcaag attctcgacc
     5401 tgatcgccgc gcgtacggga tcgcggacgg cgctgatcgc ctatgccggc agcgcgcaca
     5461 cggtcatgcc gcccaccacc gatgcggaag tgctcaggcc gtttctggag agcctggacc
     5521 ccgccatcat gcccaccggc ggggccaacg ccgccgccgt gctgcccctg gcccgatcgc
     5581 tgctcggcga ggcggcggca accggaaccc tgctgttcgt caacgacggc ttcgaccccg
     5641 tggacgttcc ggccctggcc gccttcgccc gggatcctgg caaccccccc atggcggctc
     5701 tggtcctcgg cacggaagcc ggcggcgtag cgctcatgcc cgacggatcg cccgttcaga
     5761 gcgagggcgg cgatcgcctg gacacccggg tggacgcatc gctgttgcgt cgcgccgagt
     5821 ccgagggcgg cgtcaccatc gtccgcaccg gtccggggga cgccgacgtc cgccggttgc
     5881 tccgcgtggt ggagtcgaac ctgcgccagg cggacgaccc ggacgccacc tggcgcgatc
     5941 agggctggtg gctcttgtgg ccggccgcgc tgctgtcgct gctgtggttc cgccgcggat
     6001 ggaccatgca gtggtagccc gactggtaat cattaccctg gcagccgcgg gcatcctcgg
     6061 ggtgctaacg tggcaacgag gcgacttcac cgggctgtgg ctcactccgg accagcaggc
     6121 cttgaaagcc tacgaacggc gggaatttcg cgacgccttc gatgcgttcg aggacccggc
     6181 gtggaagggc gttgccgcct atcgcagcgg cctctacggc gagtccgccg acgccttcgg
     6241 ccgcatcgac agcgcccggg gcttctacaa tcgcggcaac gccttcatga aaggccggga
     6301 gtacggcaag gccatcaccg cctacgagca ggccgtggcc gacgatccgg actggctgga
     6361 agcgcgggag aacctcgctc tggcccgcca cgtgctcgac tacatcgaac gggtccgcga
     6421 gcagtcggac accggcgacg acactgagct cggcgccgac gactacgtct tcgacaacac
     6481 ccgcaaaaaa ggggtggaaa tcgagatcac ccaggagagc accatcgagc tcgagtccgc
     6541 cgagaagtgg atgcgctcgg tggacaccca gac
//
LOCUS       DBNHKV010000144         1828 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_304667, whole genome shotgun sequence.
ACCESSION   DBNHKV010000144 DBNHKV010000000
VERSION     DBNHKV010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_304667"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..1143
                     /locus_tag="AC16PP_04725"
     CDS             13..1143
                     /locus_tag="AC16PP_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005230 - extracellular ligand-gated
                     monoatomic ion channel activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligand-gated ion channel"
                     /protein_id="HIR6480962.1"
                     /translation="MSPTQRISTLLTVLLLLLLVGWRGLAAVYQASGAVTYEHNPGEL
                     TESLSPQIPIAEATAGQHVIFMGMHIERIYELSLQSRTFTADGYVWIEWSRRTQQFME
                     AQGLRASELIHLVNRIERWDSSFEPETEEPAILSAGRRYQRFHFSSRFHDDQINFERD
                     PFDVLILPIMVEIAPDVLSDKYAQALLYPHQQQDGLLGESGSLSGYRLQGASLQALLH
                     RYPSRFGSWYQPTKSLVRLEIQYRADYWSAFVRWVLPILVVMSIVILTPTVVGSHSDI
                     RLAVPTTALLALVFMHQAYRSGLPPLPYLTLLDAMFSWGYLTCVGLFALFTWGTNVYA
                     SAPEHKKLAAATQIDRVDVVFQVLSLGTFVVLILVLWLSTGS"
     gene            complement(1310..1735)
                     /locus_tag="AC16PP_04730"
     CDS             complement(1310..1735)
                     /locus_tag="AC16PP_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480963.1"
                     /translation="MSVQVRQYQRSSIAEGRSKGYELHSIWRNTIVQNTDAAAALAKA
                     NSGESLSERERVQLGFFADELFISSAVSDQFSRHSGTFYDDEAYWRYLADILQRNPGL
                     LPEWDRIQKSVMDLNFPEYRAQVNAALADLRPGVAKADS"
BASE COUNT          333 a          532 c          539 g          424 t
ORIGIN      
        1 cggagacgac ccatgagccc gacgcagcgg atcagcactc tcctgacggt gctgctgctt
       61 ctcttgctgg ttggttggcg agggctcgcg gccgtgtacc aggcatcggg ggccgtgacg
      121 tatgaacata acccgggcga actcaccgag tcgttgagcc cccagattcc gatcgctgag
      181 gcgacggcgg gtcagcacgt gatttttatg ggcatgcaca tagagaggat ctacgaactc
      241 tccctgcagt cgcggacgtt cacggcggat ggctatgtct ggatcgaatg gtcgaggcgt
      301 acgcaacagt tcatggaggc ccagggactg cgggcgtcgg agctcattca tctggtcaac
      361 cggattgaac ggtgggactc gagtttcgag ccggaaacgg aggagcccgc gatactctcg
      421 gcggggcgcc ggtatcagcg attccacttc tcaagtcgtt tccacgacga tcagataaac
      481 tttgagcgcg atcccttcga tgtcctgatc cttcccatca tggtcgagat cgcgccagat
      541 gttctgtccg acaagtacgc ccaggccctg ctctaccccc accaacagca ggacgggttg
      601 ctcggtgagt cgggcagcct gagtggctac cggctacagg gtgcttcact gcaggcgctg
      661 ctgcaccgct atccgtcgcg ctttgggtct tggtatcaac ccacgaaaag cttggtccgg
      721 ctggaaatcc aataccgggc tgactactgg agcgcctttg tgcgctgggt cctgcctatc
      781 ctggtggtca tgtccatcgt aatcttgacg ccgacggtcg tcgggtccca ctctgatatc
      841 cggctggccg tgccgacgac cgccctgctg gcattggtct tcatgcacca ggcgtatcgg
      901 tccggccttc ccccgctccc ctacctgacg ctgttggacg ccatgttcag ctggggatac
      961 ctgacttgtg tcgggctgtt cgctctgttc acgtggggca ccaacgtgta cgccagtgct
     1021 cctgagcaca aaaaactggc cgctgcgact cagatcgatc gcgtcgacgt ggtcttccag
     1081 gtgctgtcgt taggcacttt cgtggtccta atactggtgc tgtggttgag caccggctcg
     1141 tgatgggatc aagtgaatag ggaccatacc ggccggatcg ctcgaacgga ggtggtaggc
     1201 tccgggcacg cgcggtcttc gtagttccgt cctggctgat cagggatctt gactgggtcc
     1261 ggtgcgaatg agcgaggccg gtcctgatgt ctcgtgaacg ttcgtcaaat caggaatcgg
     1321 cctttgctac gccgggtctg aggtctgcga gagccgcatt gacctgcgcc cgatactccg
     1381 ggaaattcag atccataacg cttttctgga ttctgtccca ctcaggcagg agtcctggat
     1441 tcctctgaag gatatctgcc agatacctcc agtaagcctc gtcgtcatag aaagtgcctg
     1501 agtggcggct gaattgatca gatacggctg aagagatgaa tagttcgtct gcaaaaaatc
     1561 ccagctggac tcgttctcgt tccgagagac tctctccgct gtttgccttg gccagcgcag
     1621 cagcagcatc ggtattctgg acgatcgtgt ttctccaaat cgaatgcagc tcgtatccct
     1681 tgctgcggcc ttcggctatg gagcttcttt ggtattgacg tacttgaaca ctcatcaatc
     1741 ccaaccgttg ccttcgctag gttggacctg ccgctgaaag agcagtcagg tcggtccaca
     1801 tcatctcgta gtactcgccg ccgccgtc
//
LOCUS       DBNHKV010000145         3076 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_658760, whole genome shotgun sequence.
ACCESSION   DBNHKV010000145 DBNHKV010000000
VERSION     DBNHKV010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_658760"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="AC16PP_04735"
     CDS             complement(<1..398)
                     /locus_tag="AC16PP_04735"
                     /inference="COORDINATES: protein motif:HMM:NF023299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HIR6480964.1"
                     /translation="MASADGSRGLIKTPVAKLSIGMYVAKLDRPWLESSFEVQGFYVN
                     NRALIEKLGAECEFVFVDPRRFKEIAEKPRLRVVAHNNVPRPASEGPAPTGRAKLRPV
                     KPRSPAVYEDTVETAAEIGVAQTSIDQAIE"
     gene            608..1039
                     /locus_tag="AC16PP_04740"
     CDS             608..1039
                     /locus_tag="AC16PP_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480965.1"
                     /translation="MCKPKPVHARNFLVAIALTFLFLGNAGCQSMDREEIAWQALHAV
                     DVAQTMSAARDPCYREAAWLTRNIIGEQPSEVEVLAWGVGTAIIHRWVGQVLERRDAP
                     RWVRVAWDLGTIGYTGHTVVDNHRNGVRIWGDNVEHDGCSA"
     gene            1041..2459
                     /locus_tag="AC16PP_04745"
     CDS             1041..2459
                     /locus_tag="AC16PP_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR6480966.1"
                     /translation="MSDRAGSGTDVAELFSRFGVPGGAVVLVSPRGEESLTYGSVSVA
                     RPGPVGANTLFNVGSVAKTIVAAATGLLVAERRMQLDDRLVDWVPEFTFPEPHLRTRI
                     TLRDCLAHRLGLAREGFWEWGARESLELTTLLGRLRHASMARPYRSGYAYSNVGYAAV
                     ALAASRAAGMPFLDYLRLWLDLPGDACLTGGRAARESERLSACHVLTPGPRAVRPHFA
                     DNVLGGSGIWASADFARVWLRLQLGATPTSGRCRQLPGRIRRELRLPHQRVPLADRPF
                     GVGGEACSAVHYALGWFVTRYRGHRVLRHSGAEVGSSAHLAIAPDAGIGVFVMANAES
                     PAADVIGLTLIDHLLGLRPLPWATRAPMGTRAPNGSQPRLNVRSTRAHPDGLRGLTGT
                     FRSPANGPVRITVQHGRLQMAFRDAPRFDGELVPLAGGWFRPRPADPGVRSLMEEPVR
                     FEDRSDGSCALRLGGLGTWQRR"
     gene            2535..2969
                     /locus_tag="AC16PP_04750"
     CDS             2535..2969
                     /locus_tag="AC16PP_04750"
                     /inference="COORDINATES: protein motif:HMM:NF037928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HIR6480967.1"
                     /translation="MAQTWLIVADGARARFFERRSASDLSEFDVMISPEGRSEERSLH
                     SDRPGRSFDSRGDGRHAMEPAHSARDHEAEAFAKRVCKRLDEARLAGSVDKLVLIAPP
                     RFLGHLRASLSKGTQGIVAHSIDKELTQASPDEIAEHIPGVL"
BASE COUNT          500 a          988 c         1041 g          547 t
ORIGIN      
        1 atctctatgg cctggtcgat tgaagtctgg gcgacgccga tctccgcggc ggtctccacg
       61 gtgtcctcgt aaacggccgg cgatcgcggt ttgactggtc tcagcttggc gcgccccgtc
      121 ggggccggcc cttccgacgc cgggcgcggg acgttgttgt gggccaccac ccgcaaccgg
      181 ggcttctccg cgatctcctt gaaccgtctg gggtcgacga atacgaactc gcattcggcg
      241 ccgagctttt cgatcagcgc gcggttgttc acgtagaagc cctgcacctc gaacgaggac
      301 tccagccacg ggcgatcgag cttggctacg tacatgccaa ttgagagttt ggcgacaggc
      361 gtctttatca ggccccgact gccgtccgcg ctagccatcc tggcgtcctt cgacccaata
      421 accaacacac tagggtagtc gctgtgtgga aactctctgt gatccagttc acccggacgt
      481 caacgcgccc gacttctgct cacatgtttc tacctacagc ggacaaagcg ggtacgaagc
      541 caggacagca atccggcgat gccagagcca ctattgaagg cgagaacccc aaaaggagcg
      601 gcagcttatg tgcaagccga agcccgttca tgcgagaaat tttctcgtcg ccatcgccct
      661 gacgtttctc tttctgggaa acgcgggctg ccagtccatg gatcgtgaag agattgcctg
      721 gcaggcactc cacgcggtag acgtggcgca gaccatgagc gccgcccgcg acccctgcta
      781 tcgggaagcc gcatggctga cccggaacat cattggcgag cagcccagcg aggtcgaggt
      841 gctggcctgg ggcgtgggca cggcgatcat tcaccgctgg gtcggtcaag tgctcgaacg
      901 ccgcgacgcg ccgagatggg tgcgggtggc ctgggatctg ggaacgatcg gctacaccgg
      961 ccacacggtg gtggacaacc accgcaacgg tgtgcgcatc tggggcgaca acgtcgagca
     1021 cgatgggtgc agcgcgtgac gtgtccgaca gggcagggag cgggacagac gtagccgagc
     1081 tgttcagccg gttcggcgtt cccggcgggg ccgtcgtgct ggtgtccccg aggggggagg
     1141 agtcgctcac ctacggaagc gtatccgtcg cccgacccgg cccggtcggg gcgaacaccc
     1201 tgttcaacgt cggctcggtg gccaagacca tcgtggccgc tgccaccggc ttgctggtgg
     1261 ccgagcgccg aatgcagctc gatgatcggc tggtggactg ggttcccgag ttcacgtttc
     1321 ccgaaccgca ccttcgcacc cggatcacgc tgcgcgactg cctggcccac aggctgggtc
     1381 tggccaggga agggttctgg gagtggggcg ctcgggagtc cctcgagctc acgaccctgc
     1441 tcgggcgact gcgacatgcc tccatggccc ggccctatcg ttccggatat gcctactcga
     1501 acgtcggcta tgcagccgtg gccctggccg cctcccgggc ggccgggatg ccgtttctgg
     1561 actaccttcg gctctggctg gatcttccgg gagacgcttg cctgaccggg ggccgcgcgg
     1621 ctcgcgagag cgaaaggctc tccgcctgcc acgttctgac gccgggtcct cgtgcggtcc
     1681 gcccgcactt cgccgacaac gttctggggg gctccggaat ctgggcatcc gcggacttcg
     1741 cccgggtgtg gctgcgcttg cagctgggcg cgacgccgac gagcggcagg tgtcggcagc
     1801 tgcccggccg catcagacgg gagctgcgcc tgcctcacca gcgggtgccg ctggcagaca
     1861 gaccattcgg ggtcggtggc gaagcgtgct cagcggtgca ttacgccctg gggtggtttg
     1921 tcacgcgcta tcgcggccat cgcgtgctcc gccacagcgg ggctgaagtc gggtcgagcg
     1981 cccatctcgc catcgcgccc gatgcgggca tcggcgtgtt cgtgatggcc aatgccgaat
     2041 ctccggccgc ggacgtgatc gggctgacgc tcatcgacca tctgctgggt ctgcgccctc
     2101 tgccatgggc gacccgcgcg cccatgggaa ctcgagcgcc gaacggctcg cagccgcgcc
     2161 tgaacgtgcg ctccacgcgg gcccatccgg atggtcttcg cgggctgacg ggaacgttcc
     2221 ggagcccggc caacgggccc gtgcggatca ccgtgcagca cgggcggctg cagatggcct
     2281 tccgtgacgc gccgcgattc gatggcgagc tcgtgccgct ggccggcggg tggtttcggc
     2341 cccgcccggc ggatccgggc gtgcgctcgc tcatggagga accggtccgc ttcgaagatc
     2401 gaagtgatgg atcctgtgcg ttgcgccttg gcgggctcgg tacctggcag cggcgctgag
     2461 tacgcggaaa tccttaaaga cactgcggca cgacgcaggc aacctctcgt caggttcaaa
     2521 gacgggaggc ttacgtggca cagacttggt tgatcgttgc ggatggcgcc cgggctcggt
     2581 ttttcgaacg caggagcgct tcggatctca gcgagttcga cgtcatgatt tcgcccgagg
     2641 ggcgatccga agaacgctcg ctgcactcgg accgtcccgg ccgatcgttt gattcccgcg
     2701 gtgacggaag gcatgccatg gagcccgccc acagcgcccg ggatcacgaa gcggaggcgt
     2761 tcgcaaagcg cgtctgcaaa aggctcgacg aagcgcgcct tgcgggatcg gtggacaagc
     2821 tcgttctcat cgcccccccc cgttttctgg ggcacctgcg agcgagcctc agcaagggta
     2881 ctcagggtat agtcgcgcac agcatcgaca aggaattgac gcaggcctcg cccgacgaga
     2941 ttgccgagca cataccgggc gtcctctagg gcgcctgaca acaactctat taggagtgat
     3001 catggatatt gatgaagtgg tttcagccct gcgcacggaa cgggacgagt tgagggtgcg
     3061 gttgcacctg ctcaag
//
LOCUS       DBNHKV010000146         2808 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1083564, whole genome shotgun sequence.
ACCESSION   DBNHKV010000146 DBNHKV010000000
VERSION     DBNHKV010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1083564"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..1383)
                     /gene="ribB"
                     /locus_tag="AC16PP_04755"
     CDS             complement(289..1383)
                     /gene="ribB"
                     /locus_tag="AC16PP_04755"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HIR6480968.1"
                     /translation="MSTQFDTIEDALAAIRSGEMVVVVDDDDRENEGDLIMAASKATS
                     EQVAFMIRHTSGILCAPILEDHAKQLHLDPMVARNDAPMSTAFTVSIDYRQGLTTGIS
                     AEERAATVQALTSSNVAAEDFVRPGHIFPLVARQGGVLVRSGHTEAGTDLAGLAGLPP
                     VGLLAELVNDDGTVQRLPELKEFAATHGLKIISIADLIAYRQMREQLVERTMEFAVHT
                     RIGEARAVAYKTKFEDAEHIALVFGEIGDEAVPVRIHREKLIDDIFGPQSNHPLGLLD
                     VALQRIQDLGGGVLIYLRSGFVGVPLEKLVDQSKEDERRAQWLEIGVGAQILRDLGLS
                     RIRLIAGREVDYVGVEGFGLVLESTELLND"
     gene            complement(1483..1698)
                     /locus_tag="AC16PP_04760"
     CDS             complement(1483..1698)
                     /locus_tag="AC16PP_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480969.1"
                     /translation="MSAYSIAREHLDVVASLAADNGVDLEAALRAVIGEAVERYRDLK
                     GADDLRAVLQYQLDNASGNEDYEFMRP"
     gene            complement(1731..>2808)
                     /locus_tag="AC16PP_04765"
     CDS             complement(1731..>2808)
                     /locus_tag="AC16PP_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6480970.1"
                     /translation="PRGETLRSRGETTGASYPFAMLNGNKECISLNFRSARGQALLRD
                     LVMHVDVVLENFAPGTLERYGVGARTLRGLNPELIYASSTGYGRSGAHRDYRGMDVTI
                     QAMTGVMSITGEGDGPPLKAGPALADFLGGVHLYGGIVTALYRRSQTGEGAVIDISMQ
                     DCVFPSLTTALGAYFVAGGQQGPRTGNRHQGLSVAPYNVYAARDGYVAIICIREGHWR
                     GLTRAMGRPELAEDAQFADMRARAQNMDAVDALVEGWTRTLDREEIFARAQAEGVICA
                     PVQTLEDVINDPHLHERGSLHWHEHPRLGPIVLFNSPLRFEGVDLPEVAEVPYVGAHN
                     AAVYSEFLGLDEDDIAELKQAEAI"
BASE COUNT          485 a          940 c          894 g          489 t
ORIGIN      
        1 atacagtact catggtcatt agaacgtcga caccaggccc gtgctcacgc tagatgacat
       61 cgctcgcagt ttccaggtct acgccgtgtg cgcgccgtga cagcgcacgc agccgctgcc
      121 gttggcagcg ctgatcgccc gattcggcgg ggacctgccg gtgacggccc tgcgagcgcg
      181 gcttcgatgt gagacgtgca ggcaacgaag cgcagacatg cgcatcgtgt acgtcggccc
      241 ctgcgggtcc gccgccggct tccactaccg ccgctgacga ggggacggtc agtcgttgag
      301 aagctcggtg gattcgagaa ccaggccgaa tccttctacg cccacgtagt cgacctccct
      361 gcccgcgatc agccggatgc gcgacaggcc cagatcacgc aggatctgcg cgcccacgcc
      421 gatctccagc cactgagccc gacgctcgtc ctccttggat tgatcgacca gcttctcgag
      481 cggcaccccg acgaagcccg agcgcaggta gatgagcacg ccgccgccca gatcctggat
      541 tcgctgcaaa gcgacgtcca gaaggccgag cgggtgattg ctctgcgggc cgaagatgtc
      601 gtcgatgagc ttttcccggt gaatgcgaac gggtacggcc tcgtcgccga tctcgccgaa
      661 tacgagcgcg atgtgctcgg cgtcctcgaa cttggtcttg taggccacgg ccctggcctc
      721 gccgatgcgg gtgtgcaccg cgaactccat cgtcctttcc accagctgct cgcgcatctg
      781 ccggtaggcg atgaggtcgg cgatggaaat gatcttcagg ccgtgggtgg cggcgaactc
      841 cttcagctcc gggaggcgct gcacggtgcc gtcgtcgttg accagctccg ccagcaggcc
      901 cacggggggc aggccggcga gaccggccag gtccgtgccc gcctcggtat gcccggaccg
      961 gaccagaacg ccgccctggc gagccaccag cgggaatatg tggccgggac gaacgaagtc
     1021 ctcggccgcc acgttgctcg aggtcagcgc ctgaacggtg gccgcccgct cctctgccga
     1081 tatgcccgtg gtgaggccct gtcgatagtc gatggaaact gtgaaagccg tgctcatggg
     1141 cgcatcgttg cgcgccacca tgggatccag atgcagctgc ttggcgtgat cttcgaggat
     1201 gggcgcgcac aggataccgc tggtatggcg gatcatgaac gcgacctgct ccgacgtcgc
     1261 cttgctggcg gccatgatca gatcgccttc gttttcccga tcgtcgtcgt ccaccacaac
     1321 caccatctcg cccgagcgga tggcggcaag ggcatcttcg atggtgtcga attgcgtgct
     1381 caactgcgct tgaccttaat tcacaacggg ttcgaacgac ccggggattg ccacgagcgc
     1441 gctagattac cacgccacgg ggaagggtat cgaatggccc gttcaggggc gcatgaactc
     1501 gtagtcctcg ttgccggaag cgttgtcgag ctggtattgc agaacggccc tgagatcgtc
     1561 ggcgcctttg aggtcccggt aacgctccac cgcctcgccg atgaccgcgc gaagtgcagc
     1621 ctcgagatcc acgccattgt ccgccgccag agaagctacg acatctaagt gctcacgggc
     1681 gatgctgtag gcgctcaaac caacctcctt actttcaacg aagctttcga tcagatcgct
     1741 tcggcctgct tcagctcggc aatgtcatcc tcgtcgagac cgaggaattc cgagtatacg
     1801 gcggcgttgt gagcgcccac ataagggact tccgcaacct ccggcagatc cacgccttcg
     1861 aaccgcagag gtgagttgaa aaggacgatg ggacccaggc gcggatgttc gtgccagtga
     1921 agggagccgc gctcgtggag gtgaggatcg ttgatcacgt cttccagcgt ctgaaccggc
     1981 gcgcagatca cgccttcggc ctgggcccgg gcaaagatct cttcccggtc gagggtccgg
     2041 gtccagccct ccaccagcgc gtccaccgca tccatgttct gcgccctggc acgcatgtcc
     2101 gcaaactgcg catcctcggc cagctccggg cgacccattg cccgcgtcag cccacgccag
     2161 tggccctcgc gtatgcagat gatggcgaca tagccatccc gggcagcgta aacgttgtag
     2221 ggagcgacgc tcaggccctg gtgccggttc cccgtgcgcg gaccctgctg cccgcccgcc
     2281 acgaagtagg cgcccagcgc ggtggtcagg gaaggaaaga cgcagtcctg catggagatg
     2341 tcgatgaccg caccctcacc ggtctggctg cgacgataaa gcgcggtgac gatcccgccg
     2401 tagaggtgca cgccgcccag gaagtccgcc agggcaggcc ccgccttcag gggaggtccg
     2461 tcgccctccc cggtgatgct catgaccccc gtcatcgcct gaatggtgac gtccatgccg
     2521 cggtagtcgc ggtgcgcgcc cgaccggccg tagcccgtgc tcgacgcgta gatgagctcg
     2581 gggttgaggc cacgcagggt tctggccccc accccgtagc gctccagcgt accgggcgcg
     2641 aaattctcca gcaccacgtc gacgtgcatg accaggtccc gcagcagcgc ctggccgcga
     2701 gcggagcgga agttcagcga gatgcattcc ttgttcccgt tgagcatggc gaacgggtag
     2761 ctcgcgccgg tcgtctcgcc ccggctgcgc agggtctcgc cccgcggc
//
LOCUS       DBNHKV010000147         3469 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1580149, whole genome shotgun sequence.
ACCESSION   DBNHKV010000147 DBNHKV010000000
VERSION     DBNHKV010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1580149"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="AC16PP_04770"
     CDS             <1..496
                     /locus_tag="AC16PP_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480971.1"
                     /translation="SAGVLSGPVVGGWLKRRGYADYPLRLATGAAAAVALSMVTLPFQ
                     TSGAGALVCIGLASFFVTLPLALMTFVTQTVTPRNMRGVLAGFFVVGTNVIGLGLGPT
                     LVAATTDYVLGDPGKVHVSLMIISVCVAPVAMLLLWSGMRALGEWERQNQGSPDVEDS
                     TAGR"
     gene            complement(545..2182)
                     /locus_tag="AC16PP_04775"
     CDS             complement(545..2182)
                     /locus_tag="AC16PP_04775"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6480972.1"
                     /translation="MAGLTALSAAICLSGAAVAATSDLNPIFKAWNQADAPGLVAGVF
                     RDGERLWMGSFGLSNLEFDAPNTPQTRFNAGSVAKQFTATAILRLEQAEELSLDDPIR
                     RYVPELPEVARSITLRHLLHHTSGLRDVWALTDLGGWQPADVRTPTQALRILARQRAL
                     NFLPGNGFGYSNSGYLLLAEVIRRTTGRPFPDWMRQHVLEPAGMEDTYFYEDPIKLLP
                     GVASPYRFLGRSRGFARDDLNSGLAGSGNLVTTAADLGRWSAHLLTSKIGGAPLVERL
                     SEQGILPDGRKTGYGMGLFVGTHRGLPVVSHGGANAGYRSELLMFPDQRVAVVVLGNV
                     NTVSAERLARSMADVVLAEDLPAPSPLPETTPVPMALPPASFAGRYALGQMLLEIRES
                     DGSLYFRIGGGDLRPLTATGPVTFATSEPGVSLEFQLDENRSITGVDLLAAGRTLPAE
                     RLEPEMLTATDARAYEGRYHSEELDTSYRVVYENGHLVARHLRGSDIVLTAFGQDRFL
                     ESAGGDLRVDFQRSRRGRITGFEVSVDRARAIAFDRI"
     gene            complement(2203..>3469)
                     /locus_tag="AC16PP_04780"
     CDS             complement(2203..>3469)
                     /locus_tag="AC16PP_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HIR6480973.1"
                     /translation="EFAEKEWEGMWTKVWLLLGREEELPNPGDWQCEEVGPESILMVR
                     QKDGGVKAFYNVCQHRGNPLVDGEKGSVRRFVCKYHSWAFMPDGELNFAPDADDFPEG
                     NPCGKIRLEELACETFAGFVWVNMDTECAPLKDFLGPIWDDWSRYDLHTWRRYAARTV
                     NLPCNWKVVLDNFNESYHVPTVHMAATPKTDRKKIRGNINTYFKETRFDLSDEGHNRM
                     VMEGGYGVGSTDEDGNIMEPLASLLRHWELDPDNFKEDPAGTRVALQEAKRRLGPGRG
                     YAHYDKVPNEQLTDAFHYTLFPNFAVSIWADGFHFLRARPHPTDPERCVFDNWWYASC
                     PEKDLRPVPTGMGLIDRDADVRTEVFDYGEGWVGAGIDQDVDVFVKQQRGFRSRGFKG
                     VYLSRQESRVRRYHELIDDYIEGRLPNAR"
BASE COUNT          565 a         1117 c         1143 g          644 t
ORIGIN      
        1 ctcggcgggc gtgctcagcg gtcccgtcgt gggcggctgg ctgaagcggc ggggatatgc
       61 cgactatccg ctgcgtctgg ccaccggcgc ggcggcggcc gtcgcgctga gcatggtgac
      121 cctgccgttc cagacgtcgg gcgcgggcgc gcttgtctgc atcgggcttg ccagcttttt
      181 cgtcacgctg ccgctggcgc tgatgacctt cgtcacgcag accgtgaccc cccgcaacat
      241 gcgtggcgtc ctggcgggct tcttcgtcgt cgggacgaac gtcatcgggc tggggcttgg
      301 cccgacgctt gtcgcggcga ccacggacta tgttctcggc gatcccggca aagtgcacgt
      361 atcgctgatg atcatctcgg tctgcgtggc ccccgttgcc atgttgctcc tgtggagcgg
      421 catgcgggcg cttggggagt gggagcggca gaatcaggga tcgcccgacg tggaagattc
      481 gacggctggg agatgagggt ggctgggtcg gtttccgggc cgtcgtctga tcactcctgg
      541 ggattcagat ccggtcgaag gcgatggccc gggcccgatc gacggacact tcgaagccgg
      601 tgattcgacc gcgccgggag cgctgaaagt cgaccctcag atcaccccct gctgattcca
      661 ggaaacggtc ctgaccaaag gccgtgagca cgatatcgct cccgcgcagg tgtctggcga
      721 ccaagtggcc gttctcgtaa acgacacggt atgaggtgtc gagttcctcg ctgtggtagc
      781 ggccctcgta agcccgagcg tctgtggcgg taagcatttc cggctcgagg cgctcggccg
      841 ggagcgtgcg tccggcagcg agcagatcga cgccggtaat tgatctgttc tcgtcaagct
      901 gaaattccag cgagacgccc ggttccgagg tggcgaaagt caccggtccg gtggcggtca
      961 gcgggcgcag gtcgccgccg ccaatgcgga aataaaggga cccgtcggac tcgcggatct
     1021 ccagcagcat ctggcccagg gcgtagcggc cggcaaagct tgcgggcggc agggccatgg
     1081 gcacgggtgt ggtttccgga agcggggaag gggcgggcag gtcttctgcg aggacgacgt
     1141 ccgccatgga tcgcgccagc cgctccgccg agaccgtgtt gacgttgccc agaaccacga
     1201 cggcaacccg ctgatcgggg aacatcagca actcacttcg gtatccagcg ttggcgcctc
     1261 cgtgcgagac cacgggaagg ccgcggtggg tcccgacgaa caggcccatg ccgtacccgg
     1321 tcttccgacc gtccggcaag atgccctgct cggagagccg ttcgacgagc ggagcgcccc
     1381 cgattttcga tgtcaggaga tgcgccgacc atcgcccgag gtcggctgcc gtcgtgacga
     1441 gattgccgct gcctgccagc ccgctgttga gatcgtcacg agcaaaaccc ctgcttctgc
     1501 cgaggaagcg gtacgggctg gcgacgcctg gcagcagctt gatcgggtcc tcgtagaagt
     1561 aggtatcctc catgccggcc ggttccaaga cgtgctggcg catccagtct ggaaacggcc
     1621 tgccggtggt acgtcggatc acttcggcca ggaggagata gccgctgttg gagtagccga
     1681 acccattgcc gggcaaaaaa ttcagcgccc gctggcgcgc caggatacgc agagcctggg
     1741 tcggcgtcct cacgtcggct ggctgccagc cgcctaagtc cgtgagcgcc cagacgtccc
     1801 gcagaccgct cgtgtgatga agcaggtgtc gcagggtaat ggatctggca acctctggca
     1861 gctcgggcac gtatcgacgt atcgggtcat ccaggctgag ctcctcagct tgctcgagcc
     1921 tgaggatggc cgtggcggtg aactgcttgg caacggaccc cgcgttgaaa cgggtttgcg
     1981 gggtgttggg cgcgtcgaac tccaggttcg acaggccgaa gcttcccatc cacagccgtt
     2041 cgccgtctcg gaacacgccc gcgaccaggc cgggagcatc cgcttggttc caggccttga
     2101 agatcgggtt caggtccgag gtggcagcga cggctgcccc tgacaggcaa atggctgcag
     2161 agagagcagt cagtcccgca atagcgtttg tcctcgtcga acttacctcg cgttgggcag
     2221 gcgcccctcg atgtagtcgt cgatgagctc gtgatagcgg cgcacccggc tctcctgcct
     2281 ggacaggtag acgcccttga aaccccggga ccggaagccg cgctgctgct tgacgaaaac
     2341 gtccacgtcc tgatcgatgc cggctccaac ccagccttcg ccatagtcga atacttcggt
     2401 acgcacgtcc gcgtcacggt cgatcaggcc cattccggtt ggcacgggac gcagatcctt
     2461 ctcggggcag ctggcgtacc accagttgtc gaagacgcac cgttccggat cggtgggatg
     2521 gggccgcgcc cgcagaaagt gaaagccgtc ggcccagatc gacaccgcga agttcggaaa
     2581 cagcgtgtag tgaaacgcgt ccgtcagctg ctcgttggga accttgtcgt agtgggcata
     2641 acctcttccg ggtcccagcc ggcgcttggc ctcttgcagc gccacccgcg tgcctgccgg
     2701 gtcttccttg aaattgtccg ggtcgagctc ccagtgccgc agcaggctcg ccaggggctc
     2761 catgatgttg ccgtcttcgt ccgtggaccc gacgccgtag cccccctcca tcaccatccg
     2821 gttatgaccc tcgtcggaga gatcaaagcg ggtttctttg aagtaggtgt tgatgttgcc
     2881 gcgtatcttc ttgcggtcgg ttttcggcgt ggccgccatg tgcacggtcg gcacgtggta
     2941 ggactcgttg aagttgtcca gcaccacctt ccagttgcag ggcaggttga ccgtccgcgc
     3001 cgcgtagcgc cgccaggtgt gcaggtcgta gcgggaccag tcgtcccaga tggggcccag
     3061 gaagtccttc agcggcgcgc actccgtgtc catgttcacc cacacgaacc cggcaaacgt
     3121 ctcgcaggcg agctcctcca aacggatctt cccgcacggg ttgccctcgg gaaagtcgtc
     3181 cgcgtccggc gcgaagttca gctccccgtc gggcatgaac gcccagctgt ggtacttgca
     3241 cacgaagcgc ctgacgctgc ccttctcgcc gtccaccaac gggttgccgc ggtgctggca
     3301 gacgttgtag aaggccttca cgccgccgtc cttctggcgc accatcagga tggactcggg
     3361 gcccacctcc tcgcactgcc agtcgccggg gttcggcagc tcctcttccc ggcccagcag
     3421 cagccagacc ttggtccaca tgccttccca ctctttctcg gcaaactcc
//
LOCUS       DBNHKV010000148         2199 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_162949, whole genome shotgun sequence.
ACCESSION   DBNHKV010000148 DBNHKV010000000
VERSION     DBNHKV010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_162949"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="AC16PP_04785"
     CDS             complement(<1..261)
                     /locus_tag="AC16PP_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480974.1"
                     /translation="MTELTADLIAELQRLDTPTVCNALEVVAPERRGYGYTVRPLVCT
                     RPELPPLVAVARTATIRAAHPGHLRGSDAQALRDAYYAYVDEG"
     gene            complement(459..1400)
                     /locus_tag="AC16PP_04790"
     CDS             complement(459..1400)
                     /locus_tag="AC16PP_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0050896 - response to stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6480975.1"
                     /translation="MKAFEHAIVGLDFNRDCAAAILDRATQLVDPEEIDVVHAFSGND
                     HSPVTAEEAGLMTMCADYGVASVKFVSGESAHALHEQAGKRSDLIIVGSHGRSGLRGI
                     IHSHSNATLHATPCDLLTVHLDDPPQSRSPPYRSVLVAIHLGPVTLPSKCEQCSFWPS
                     AQEENRLNRGHRFMNALKPIKLIPRHSHASEESHYPLCHVPTEVILHARKIAQQSGAE
                     LSLCHVYMGYDVDTQQMERELLDSLGELYEVPEDSRYSVPGSVSGAIHRLASRIGADL
                     VVLGTHGRSGPSLAVGSTANAVLHGATCDQLSVRIGA"
     gene            complement(1673..>2199)
                     /locus_tag="AC16PP_04795"
     CDS             complement(1673..>2199)
                     /locus_tag="AC16PP_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR6480976.1"
                     /translation="INYRSLLESVMGSAKPMAYFEEAQARAADLDPEDMSDEAVTLRG
                     IVMHHRDWIRHNFRRERLRRAWDEFFAEWDVLVCPQMATPAFPHDHRPFAERTINVDG
                     VEQPYWQQLFWAGMVSNAYLPSTVFPTGPGSDGLPIGLQAISGPYRDYRTIEFASLMA
                     EEIGGFAAPPAMRG"
BASE COUNT          388 a          656 c          734 g          421 t
ORIGIN      
        1 cccctcgtcc acgtaggcgt agtaggcgtc acgcagtgcc tgtgcatcgc tgccgcgcag
       61 gtgtccgggg tgcgcggcac ggatggtggc cgtgcgcgcc accgccacca ggggcggcag
      121 ctccgggcgg gtgcagacaa gggggcgaac ggtgtagccg tagccgcgcc gctccggggc
      181 aaccacctcc agcgcgttgc agacggtggg cgtatccaga cgctgaagct cggcgatgag
      241 gtccgcggtg agttcggtca tgggctcttc ctgacggttc catacgctga aagtatcacg
      301 cccgcgcgcg gatgggccaa gcgctgcgtc ggagctcttt caccggcgtg cacgttgcga
      361 acggaatgct gtttatcgtc ttatcgtccg gttaatcaac gtgcgcaagt tcgccgactg
      421 agcccggcct gacgaggtgc ccgccgacgt gccgggtgtc aggcgccgat tcgcaccgac
      481 agctgatcgc acgttgcgcc gtgcaggacc gcattggcgg tcgaacccac cgcaagactc
      541 gggcctgagc gtccgtgcgt gccgagcacg acgagatctg ctccgatgcg actggccagc
      601 cggtgaattg cacctgagac cgatccgggc accgagtacc ggctatcctc gggaacctcg
      661 tagagctcac cgaggctgtc gagcagctcg cgttccattt gttgggtatc gacgtcgtag
      721 cccatgtaca cgtggcacag agagagctcg gctccgcttt gctgagcaat cttgcgggcg
      781 tgcagaatta cctcggttgg aacgtggcac agagggtagt gcgactcttc gctggcgtgt
      841 gaatggcgcg ggatcagctt tatgggtttg agggcattca tgaaccggtg gcctcggttc
      901 agtcgattct cctcttgcgc gctcggccaa aaactgcatt gctcgcattt ggaaggaagc
      961 gtgaccgggc ccaggtgaat cgcgacgaga acgcttcgat acggtggcga gcgactctgg
     1021 ggcggatcgt ccaggtgcac cgtcagcaga tcgcaggggg ttgcgtgcag cgtggcgttg
     1081 gaatgggagt gaatgatgcc ccgcagcccg gaccggccgt gggagccgac aatgattaga
     1141 tcgcttcgtt tcccggcttg ctcgtgaagt gcgtgggcag attcgccgct gacgaacttg
     1201 acggaagcga caccgtaatc ggcgcacatc gtcatcagtc cggcctcctc ggcagtgacg
     1261 ggagagtgat cattcccgga gaaggcgtgc acgacgtcga tctcttcagg gtcgaccagc
     1321 tgagtggccc gatcaaggat cgccgccgcg caatcgcggt tgaaatctaa cccgactatc
     1381 gcgtgttcaa aggctttcat gccaacgcgt ccgtttgggt agtaacaaac agtctaacac
     1441 cctcaagcca tggtctgaac gacccctttg gaataacccc cggttttcta attagcccga
     1501 tgaatataag aatgattttc gattaggaaa tctgaaggtt tcagtggagt agcgctgcct
     1561 gatcagaagc gggcgagaac ggttcgcatg ggctcaccat gcggggccac ggccattcgc
     1621 ggttctggca gcagcgggtg gtcgaggatg tggggcgcct tacgccccca actcatcctc
     1681 tcatggcggg cggggctgcg aacccgccga tctcctcggc catcaggctc gcgaactcga
     1741 tggtccgata gtcgcggtac ggcccgctga tggcctgcag gccgattggc agcccgtcgc
     1801 tgccgggccc ggtgggaaac accgtgctcg gcaggtaggc gttgctgacc atgcccgccc
     1861 agaagagctg ctgccagtag ggctgctcga cgccgtctac gttgatcgtc cgctccgcga
     1921 aaggccggtg gtcgtgggga aatgccgggg tggccatctg cgggcagacg aggacgtccc
     1981 actcggcgaa gaactcgtcc cacgcccggc gcaggcgctc gcggcggaag ttgtgacgga
     2041 tccagtcccg gtggtgcatg acgatgcccc gcaaggtcac tgcctcgtcg gacatgtctt
     2101 cgggatccag gtccgctgcc ctggcctggg cctcctcgaa gtaagccatg ggcttggcgc
     2161 tccccatcac ggattcgagc aggctgcggt agttgatgt
//
LOCUS       DBNHKV010000149         7847 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1083893, whole genome shotgun sequence.
ACCESSION   DBNHKV010000149 DBNHKV010000000
VERSION     DBNHKV010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1083893"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2026
                     /locus_tag="AC16PP_04800"
     CDS             <1..2026
                     /locus_tag="AC16PP_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HIR6480977.1"
                     /translation="DLIRRAEGAAMVGPEGASINQRGYVYMAAQIGEPGSRAGVLFAV
                     VSPDFFLSGLAWLDERIGRVRIEQLSESVDPIEIFGWGGQSDPAAAEIVTLENQKWNL
                     VFEPGSAILQPVTKMVDLALPFGVALLLVIAGGYLSTTRVTKRLRQAAAAMPVQAQPP
                     EEAPAAETAVEEEAEPVGEEAEPGAGAASTLPSRGFTDDAKDNFGIEVSEEVSLGEMG
                     IDLDPQIFRAYDIRGIVTDNLTEDVVYWIGRAFAAEALAQEQTRAAVGRDGRHSSEAL
                     RTALIKGLTDGGLDVADVGEVPTPLLYFATHALDLGTGIMITGSHNPPEYNGLKMMIA
                     GDTLAEERIQRLKERLENNELSEGEGDVEEVDIVRYYIDSALDDIVVAQQLKVVVDCG
                     NGVAGAVAPVLLEEAGCEVVPLYCDVNGDFPNHHPDPADPKNLEDLITVVQAESADLG
                     LAFDGDGDRLGVVTSSGQIIWPDRLLMLFAQDIVSRNPGADIVYDVKCSRHLNAIIGD
                     HGGRPIMWKTGHSHIKAKLKETGALLGGEFSGHICFNDRWYGFDDALYSAARLLEILG
                     AEGGTVDEVFEQFPTGQSTPELKIQTTEDAKFGIIEELSRSADFGDGTVTSIDGVRVD
                     YPDGWGLIRASNTSPVLTLRFEADDAEALARIQSDFAVQIAKVDPSLKIS"
     gene            2031..2951
                     /gene="argB"
                     /locus_tag="AC16PP_04805"
     CDS             2031..2951
                     /gene="argB"
                     /locus_tag="AC16PP_04805"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR6480978.1"
                     /translation="MPISESQARNIASVLTEALPYIQRFHGGTVVVKYGGNAMVDETL
                     KTTFARDVVLLKLVGLNPIIVHGGGPQIGELLDRLNIPTRFVKGMRVTDAETMDVVEM
                     VLGGLVNQDIVSLINKNGGRAVGITGKDGDLIRAHRLALSADAAELAPSEIIDIGHVG
                     EVESVNPAVLDALVRSNFIPVIAPIGVGPDGESYNINADLVAGRLAEELRAEKLILLT
                     NTAGILDANGQTLTSLSVAEINELIAAGTIGSGMLPKAQCAVSAAERGVGSTQIIDGT
                     IPHALLLEIFTDAGIGTQITTAEASGSDAS"
     gene            2941..3525
                     /gene="slmA"
                     /locus_tag="AC16PP_04810"
     CDS             2941..3525
                     /gene="slmA"
                     /locus_tag="AC16PP_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HIR6480979.1"
                     /translation="MPPRSGRRDEILSAFARMLESNPGTRITTAALAGQIGVSEAALY
                     RHFPSKAKMIEGLIEFIEESLFSRINRILAEEASAASRCRAVLWLLLSFAERNPGLAR
                     LLVGDALQGETQRLRARMRQVFERLETELRSIIRDWSAARVPAPTLGATTAANLLLAS
                     AEGRISQFVRSEFRALPTSGWEEQWKALEGAVFG"
     gene            complement(3550..4155)
                     /locus_tag="AC16PP_04815"
     CDS             complement(3550..4155)
                     /locus_tag="AC16PP_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480980.1"
                     /translation="MVAILLMPSWASANRLYRYLDASGHIAISHTIPSDRVKFGYEIV
                     DAGSGRVIEVVEAQKSPEEIARLEQEAAARKACEDALERVYSVYQSELDIENAEEQML
                     ESLEQRIANAEFNLERAQSQKDGFEATAARLERSGKELDTSLIANIERSQVQVRSLET
                     EIVQRRHEQAKAKERFARDRALFRQEICVEQTAISAVTETN"
     gene            complement(4251..4919)
                     /gene="pyrE"
                     /locus_tag="AC16PP_04820"
     CDS             complement(4251..4919)
                     /gene="pyrE"
                     /locus_tag="AC16PP_04820"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR6480981.1"
                     /translation="MSASRNDRSLVAFLIEQGVLAFGDFTLKSGRRSPYFFNLGALNT
                     GSALARLGAAYAERIRRLDVPFDVVFGPAYKGIPIAVSTAEALAALGGDVGWAFNRKE
                     AKSHGEGGLFVGADVSGRVMLVDDVLTAGTAVREAAALIGSAGAAIAGVVIALDRQER
                     VAPNSGETAVSALELELGAPVVSILKLQDVIEYLDLDVDRDKHRAELIREMKRYRDEH
                     CVLN"
     gene            complement(4916..5635)
                     /gene="rph"
                     /locus_tag="AC16PP_04825"
     CDS             complement(4916..5635)
                     /gene="rph"
                     /locus_tag="AC16PP_04825"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HIR6480982.1"
                     /translation="MTETQVQRPSGRAPSEMREVRFTRGFTKHAAGSVLVEFGDTKVI
                     CTASVENSVPGFLRGKGVGWVTAEYGMLPGATHVRTDREAARGKQGGRTQEIQRLVGR
                     ALRASVDMQALGERTIRLDCDVIQADGGTRTASITGSALALADAIASAGCNGALKELV
                     ASVSVGLWRGQPVLDLDYAEDSTAETDMNVVMLESGGFIEIQGTAEAGAFSDSELMSM
                     LALARSGTAELLRRQRQALSS"
     gene            5790..6644
                     /locus_tag="AC16PP_04830"
     CDS             5790..6644
                     /locus_tag="AC16PP_04830"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR6480983.1"
                     /translation="MTAFARVQGSAKGLSLIWEMRSVNHRFLETQFRLPEQMRPLEHG
                     LRETLRRHVKRGKFDCSLKIDRSTGGDGIELNRPLLLQLLAAVEQIRRDAPEIGPGNA
                     MDVLRWPGIIGDASTLEDEPLVQAATDLFEEALVQLIDNRQREGAQLASVISERLDEI
                     DSIAREVKALTATMSHEVRSRLTQRLNDLKVQADEGRLDQEVALLAQRADVAEELDRL
                     VIHVEEARSNLRGPGPHGRRLDFLTQELNREANTLGAKSVLAQTSQKAVDLKVAIEQI
                     REQVQNVE"
     gene            6641..7273
                     /gene="gmk"
                     /locus_tag="AC16PP_04835"
     CDS             6641..7273
                     /gene="gmk"
                     /locus_tag="AC16PP_04835"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR6480984.1"
                     /translation="MSQASRQGLLFIVSAASGTGKTSLVRALIAQDGNLAVSISHTTR
                     PRRPGDQEGVDYHFVEPKLFREMAQRGDFLEDADVFGNRYGTSKRTVDEQLRTGVDVI
                     LEIDWQGARQVRAAYPDATSIFILPPSRAALVQRLEGRGLDAANVIEARSDKAREEIS
                     HYGEYDYLVVNDDFATALSELTQIIAASRLGLQPQRERLGALLKDLLRQE"
     gene            7373..7645
                     /gene="rpoZ"
                     /locus_tag="AC16PP_04840"
     CDS             7373..7645
                     /gene="rpoZ"
                     /locus_tag="AC16PP_04840"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003195508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR6480985.1"
                     /translation="MARITVEDCLENVENRFELVMLATRRARQMRRFGAEPLVPEEND
                     KPTVIALREIAEGLISNEMLDAQEASTEDEELEVSFGIGDGETGEI"
     gene            7623..>7847
                     /locus_tag="AC16PP_04845"
     CDS             7623..>7847
                     /locus_tag="AC16PP_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480986.1"
                     /translation="MAKPARSDHQGSKHPGSRVVRKKTRAGEKPKLPQPESYFVGLLA
                     DESRRDDIAAPVSIESLTTLLSTYLEPDQVR"
BASE COUNT         1472 a         2574 c         2505 g         1296 t
ORIGIN      
        1 cgatctgatc agaagagccg agggcgcggc gatggtgggg cccgagggcg catcgatcaa
       61 tcagcgcggt tatgtctaca tggctgcgca gattggagag ccgggctccc gcgccggggt
      121 gttgttcgcc gtggtctctc cggacttttt cctgagcggg ctggcctggc tggatgagcg
      181 catcgggcgc gtgcgcatcg agcagctctc cgagagcgtc gaccccatcg agattttcgg
      241 ctggggcggc caatccgacc ccgccgccgc ggagatcgtc acgctggaga accagaaatg
      301 gaatctcgtc tttgagcccg gttcggcgat tctgcagccg gtgaccaaga tggtcgacct
      361 ggcactgcca ttcggggtcg cccttctgct ggtaatcgcc ggcggatacc tctccaccac
      421 gcgcgttacc aagcgtctgc gccaggccgc cgccgccatg ccggtgcagg cccaaccgcc
      481 cgaggaagcg ccagcggcgg agacggccgt cgaggaggaa gccgagcccg tcggcgagga
      541 ggccgagccg ggagcgggag cggcaagcac gctgcccagc cgcggcttta ccgacgacgc
      601 caaggacaac ttcggcatcg aggtcagcga ggaagtgtct ctgggcgaga tggggatcga
      661 tctggacccg cagatattcc gggcctacga cattcgcggc atcgtcaccg ataatctgac
      721 cgaggacgtc gtgtactgga tcgggcgggc gtttgccgcc gaggcgctgg cccaggagca
      781 gacccgggcg gccgtaggcc gcgatggccg acactcgagc gaggcgctgc gcaccgcgct
      841 catcaagggc ctgaccgatg gcggtctcga cgtcgccgat gtcggcgagg tgccgacccc
      901 gctgctctac tttgccaccc acgctctgga cctgggcacg ggcatcatga tcacgggcag
      961 ccacaaccca cccgagtaca acgggctgaa gatgatgatc gctggggaca ccctggcgga
     1021 agaacgcatc cagcggctca aggagcggct ggagaacaac gagctgtctg agggcgaggg
     1081 cgacgtggaa gaagtcgaca tcgtccgcta ctacatagac agcgcgctgg acgacatcgt
     1141 ggtcgcccag cagctcaagg tcgtcgtcga ctgcggcaac ggcgtggcgg gtgccgtcgc
     1201 gcccgtgctt ctggaggaag ccggctgcga ggtcgtcccg ctttactgcg acgtcaacgg
     1261 cgatttcccc aaccaccatc cggatccggc ggacccaaag aacctggaag acctgatcac
     1321 cgtggtccag gccgaatccg ccgacctggg actcgccttc gacggcgacg gcgatcgcct
     1381 gggagttgtg acctcttccg gccagatcat ctggccggac aggctgctga tgctgttcgc
     1441 ccaggatatc gtgagccgga atccgggtgc cgacatcgtc tacgacgtga agtgcagccg
     1501 gcatctgaac gccatcatcg gagaccacgg cgggcggccc atcatgtgga agaccggaca
     1561 ctcccacatc aaggcgaagc tcaaggagac cggagcgctg ctgggcggag agttcagcgg
     1621 tcacatctgc ttcaacgatc ggtggtacgg gttcgacgac gccctctact ccgcggcacg
     1681 gctgctggaa atcctcggcg ccgagggcgg caccgtggac gaggtgttcg agcagttccc
     1741 cacggggcaa agcacgcccg agctcaagat ccagaccacg gaggatgcga agttcgggat
     1801 catcgaggag ctcagcaggt cggccgattt cggcgacggc acggtcacca gcattgacgg
     1861 ggtgcgcgtc gactaccccg acggctgggg gctgatccga gcctcgaaca cgagccccgt
     1921 gctcacgcta cgtttcgagg ccgacgacgc cgaggcgctc gcgcgcattc agtcggattt
     1981 cgcggtgcag atcgcaaagg tcgacccctc gctgaagatt tcctgaagcc atgccgatct
     2041 ccgaatccca agcccgcaac atcgcgagcg tactgaccga ggcgcttcct tatatacagc
     2101 gattccacgg cggcaccgtg gtggtcaagt acggcggcaa cgccatggtg gacgagacgc
     2161 tcaaaacgac cttcgcgcgc gacgtcgtgc ttctgaagct ggtcggtctg aatcccatca
     2221 tcgtccatgg cggcgggccg cagatcggcg agctcctgga tcggctcaac attcccaccc
     2281 gcttcgtcaa aggcatgcgg gtcacggacg ccgagaccat ggacgtcgtg gagatggttc
     2341 tcggcgggct ggtgaatcag gacatcgtat ccctgatcaa caagaacggc ggtcgcgccg
     2401 tggggataac gggtaaagac ggcgatctga tcagggcgca ccggctggcg ctgagcgccg
     2461 acgcggcgga gctcgccccg tcggagatca tcgacatcgg ccacgtcggc gaggtcgaaa
     2521 gcgtcaaccc ggccgtcctg gacgccctcg tgcgcagcaa cttcatcccg gtgatcgcgc
     2581 ccattggcgt ggggcccgat ggcgagtcct acaacatcaa tgccgacctc gtcgcaggcc
     2641 gcctggcgga ggagctgcgc gcagaaaagt tgatactgct gaccaacacc gccggcatcc
     2701 tggacgccaa tggccagaca ctcacctcgc tgagcgtcgc ggagatcaac gagctcatcg
     2761 ccgcgggcac catcggctcg ggcatgctgc ccaaggcgca gtgcgccgtg tccgctgccg
     2821 agcgcggcgt agggagcaca cagatcatcg acggaaccat acctcacgct ctcctgctgg
     2881 aaatcttcac cgatgccgga atcggtacgc agatcaccac cgccgaggcg agcgggtccg
     2941 atgcctccta gatcgggccg ccgcgacgaa attctcagcg cgttcgcgcg aatgctggaa
     3001 agcaacccgg gaacgcggat aaccaccgcc gccctggccg gccagatcgg ggtatccgag
     3061 gccgccctct accggcactt tcccagcaag gcaaaaatga tcgaggggct catcgagttc
     3121 atcgaggaga gcctcttcag ccgcatcaat cgcattctcg cagaggaggc cagcgccgcg
     3181 tcccgctgcc gggccgtgct gtggctgctg ctttccttcg ccgagcggaa tccgggactg
     3241 gcgcgcttgc tggtcgggga cgccctgcag ggcgagaccc aacggcttcg ggcgcgcatg
     3301 cgccaggttt tcgagcggct ggaaaccgag cttcggtcga tcatccggga ctggtcggcc
     3361 gcccgggtgc cggcaccaac gctcggtgcc acgacggccg ccaacctgct cctggccagt
     3421 gcggagggtc gcatcagcca gttcgttcga agcgaatttc gcgcgctgcc gacctccggc
     3481 tgggaggaac agtggaaggc tctggaaggg gccgtcttcg gctagccagc caaggaacgg
     3541 caaacgccct cagttcgttt ccgtaaccgc gctgatggcc gtctgctcga cacagatttc
     3601 ctggcggaaa agagcccgat cacgggcgaa acgttccttg gctttcgcct gctcatggcg
     3661 gcgctgaacg atctccgtct ccaggctcct cacctgaacc tgggacctct cgatattggc
     3721 gatgagggag gtgtccagtt ccttgccgga gcgctcgagt cgggcagcgg tggcctcgaa
     3781 gccgtctttc tggctttgcg cgcgctcgag gttgaactcg gcgttcgcaa tgcgctgctc
     3841 tagagattcc agcatctgct cttctgcgtt ctcgatatcg agctcagact ggtacaccga
     3901 atagacgcgc tcgagcgcgt cttcgcacgc cttgcgggcg gccgcttcct gttccagcct
     3961 ggcgatttcc tcagggctct tctgggcctc cacgacctcg atcacccgcc cgctgccggc
     4021 gtcgacgatc tcgtagccga acttgacccg gtcgctgggg atggtgtgag agatcgctat
     4081 gtgaccgctg gcatcgaggt accggtacag acgattggcc gaagcccagg acggcatcag
     4141 gaggatcgcc accatgatca agaccgccgc ccgcgggcga catcggaaat tgcctcggta
     4201 tcgtgttccc atcaatcgct caatcgtcga gttatgagcg actagccggt tcagttcaga
     4261 acgcaatgct cgtcgcggta ccgcttcatt tcgcggatta gttcggctcg atgtttgtcc
     4321 cgatcgacgt ccagatcaag gtactcgatc acgtcttgga gcttgagaat gctgactacc
     4381 ggtgcgccaa gctccagctc cagggcagaa accgccgttt cccctgagtt cggggctacg
     4441 cgctcctgtc tgtcaagcgc aatgacaacg ccggcaatcg ccgcgcctgc agatccaatg
     4501 agcgcggccg cttcccgcac ggcggtaccc gcggtaagca cgtcgtccac gagcatgacc
     4561 ctgcccgaga cgtctgcgcc tacgaagagg ccgccctccc catgggactt ggcttccttc
     4621 ctgttgaatg cccaaccaac gtcgccacca agagcagcaa gcgcctccgc agtggaaacc
     4681 gcaatgggaa tgcccttgta agcgggaccg aacaccacgt cgaacggcac gtccagccgg
     4741 cgaatgcgct cggcgtaggc ggcgcccagc cgcgccaggg ctgaacccgt attcagagcc
     4801 cccaggttga agaagtaggg gcttctgcgg ccggatttca gcgtgaaatc cccgaacgcg
     4861 agaacgccct gttcgatgag aaacgccacc agggagcgat cgttccggga ggcgctcaag
     4921 agctcagcgc ctgtcgttgg cggcgcagca gctccgccgt gcccgaccga gccagggcca
     4981 gcatgctcat cagctcgctg tcgctgaacg cccccgcctc ggccgttccc tgaatctcga
     5041 tgaacccacc ggattccagc atcaccacat tcatgtcggt ctccgccgtg gagtcctcgg
     5101 cgtaatccag atccagcacg ggttgaccgc gccacagacc aaccgacacc gaggcaacca
     5161 gctccttgag cgccccgttg caaccggccg aggcgatggc gtcggccagc gccagggcgg
     5221 agcccgttat ggatgccgtc cgcgtgccgc cgtctgcctg gatcacgtcg caatccagcc
     5281 ggatggtgcg ctcgccgagc gcctgcatgt ccacggacgc ccgcaaggcg cgcccgacga
     5341 gccgctgtat ttcctgcgtg cgaccgccct gcttgccacg cgctgcctct cgatcggttc
     5401 gcacatgggt ggcgccgggg agcatgccgt actcggcagt cacccagccc acgcccttgc
     5461 ctcgcaggaa gccgggcacc gagttctcca cgctggccgt acagatcacc ttggtatcac
     5521 cgaactccac gaggacggag cccgcggcat gcttggtgaa tccgcgggtg aagcggactt
     5581 cccgcatctc tgagggcgcc cggccactgg gacgctgaac ctgtgtttcg gtcatcaaaa
     5641 tcctcgggac ggcagaagtg ggcgggcatt ataaaggcaa cggccggcag caacgcgatc
     5701 gcgcggcgcg gttggatggc agggcccccg cccgggtaga atgcgcgctc gccattttac
     5761 gtgccgctga cctaccgatg atccacagca tgaccgcatt cgcccgcgtc cagggcagcg
     5821 ccaaggggct ctcgctgatc tgggagatgc gatcagtgaa tcaccgtttt ctggaaactc
     5881 agttccggct tcccgagcag atgcgcccgt tggagcacgg cctgcgtgaa accctgcggc
     5941 gtcacgtcaa gcgcggcaag ttcgactgct cgctgaagat cgaccgaagc accggcggcg
     6001 acggtattga gctcaaccgc ccgctgctcc tccaactgct cgccgcggtc gagcagatcc
     6061 gccgggacgc tccggagata gggcccggca acgccatgga cgtcctgcgc tggccgggca
     6121 tcattggcga tgcgtcaact ctggaggacg aacccctcgt ccaggccgct actgatctgt
     6181 tcgaggaagc gctcgtccag ctcatcgaca atcggcaacg ggagggagcg cagctcgcct
     6241 ccgtcatctc cgagcggctg gacgagatcg acagcatcgc tcgcgaggtc aaagcgctga
     6301 ccgcgacgat gagtcacgag gtgcgctcca ggctgaccca acgactcaac gatctgaagg
     6361 ttcaggccga cgaaggccgg ttggaccagg aagtcgccct gctggcccag cgggccgacg
     6421 tcgccgagga gctcgaccgg ctggtcattc acgtggagga ggcccgcagc aacctgcgcg
     6481 gcccggggcc ccacgggcgc cggctcgact ttctcaccca ggagcttaac cgggaagcca
     6541 atacgctggg cgcgaagtcg gtgctggccc agacgtcgca gaaggcggtg gacctgaagg
     6601 tcgccatcga gcagatccgc gagcaggttc agaacgtcga atgagccaag cgtctcgcca
     6661 gggcctgctg ttcatcgttt ccgcagcctc tggaacaggc aagacgagcc tcgttcgtgc
     6721 gctcatcgct caggacggca acctggccgt ttcgatctcc cacaccacgc gtccgcgcag
     6781 gccgggcgat caggaaggcg tggattacca cttcgtcgaa ccgaaattgt tccgggagat
     6841 ggcgcaacga ggggatttcc tggaggacgc cgacgtcttc ggaaaccgct acggaacgtc
     6901 gaaaaggacc gtcgacgagc agctgcgcac aggcgtcgac gtcattctcg agatcgactg
     6961 gcagggtgcc cggcaggttc gcgccgctta cccggacgcg accagcattt tcatcctgcc
     7021 gcccagccgc gccgccctgg tgcagcggct ggaggggcgg ggcctggatg ccgccaacgt
     7081 catcgaggcg cgcagcgaca aagcccggga ggagatttcg cactacgggg agtacgacta
     7141 cctggtggtg aacgacgact tcgccacagc cctgagcgag ctgacgcaga tcatcgcggc
     7201 gagccggctc gggctgcagc cccagcggga gcggctgggc gcgctgctca aagacctcct
     7261 ccggcaggag tgacggcact ttacaatctt ccggaagggc ggctacactc gcgcgctcgc
     7321 tgcacaccgg ctctctcgga gccactttag aagatcgcat cagaggttcg ccatggcccg
     7381 catcaccgtc gaggattgcc tggaaaacgt agaaaaccgc ttcgagctcg tgatgctcgc
     7441 aacgcggcgg gcacgccaga tgcgccgctt cggcgcggag cccctggttc cggaagaaaa
     7501 tgacaagccc accgtgatcg ctctgcgcga aattgccgaa gggctcatat caaatgaaat
     7561 gcttgacgca caagaagcct ccacggagga tgaggaactt gaggtttctt tcggtatagg
     7621 agatggcgaa accggcgaga tctgaccacc agggctccaa gcaccccggg tctcgcgttg
     7681 tccgcaagaa aacacgcgct ggcgaaaaac cgaagctccc ccaacccgaa tcctacttcg
     7741 tcggcctgct cgccgacgag tcgaggcgcg acgacatcgc cgcgccggta tcgatcgaat
     7801 ccctgacgac cctgctgtcc acttatctgg agccagatca ggttcgc
//
LOCUS       DBNHKV010000150         5856 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_801361, whole genome shotgun sequence.
ACCESSION   DBNHKV010000150 DBNHKV010000000
VERSION     DBNHKV010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_801361"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="AC16PP_04850"
     CDS             <1..1204
                     /locus_tag="AC16PP_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HIR6480987.1"
                     /translation="AGEVMPNPFALLPERRGRAQPEPKPEPFGYGERLRVRDLTENVS
                     GLSAAALADEILLEGEGQVKALFCVGGNPMAAWPDQLKTYQAMKALELNVCLDIKMSA
                     TAKLAHFVLPPRLGLESPAISLPNENIWFYGFSTGFPEPYAMYQPQLVEPPAGSDLLE
                     EWEVFYGVAQRMGLGLTFAGEGLDMNRKPTTDELFEIMCKGSRIPLEEVKRYPHGHIF
                     SDPSITVLPKEEGCEDRLDVGHELMMRELAEVRAEPLSGHGGYAGERAFTHRLVSRRM
                     NDVYNSSGRDIPRLVRNHRYNPAYMNPDDIEALGLHDGDVVEIASDHAKILGIVEAAP
                     DIRPGVVSMAHAFGDAPEHDEDVFYIGSNTGRLTDVERNYDPITGMPRMSAIPVNVRR
                     GDQSLASR"
     gene            1244..2005
                     /locus_tag="AC16PP_04855"
     CDS             1244..2005
                     /locus_tag="AC16PP_04855"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6480988.1"
                     /translation="MELGLEGKRAIILGGSRGIGWYTARALAVEGCSVALCARDADGV
                     QAAVDALSEDPGGSHFGAAADLTDGDGTRAFVREAITALGGLDLLIHNASGFDRSGDE
                     EGWQRSFQVDVMAGVRAVEEALPALKESDAASITFIGSMASKFYFGRRSSYGPAKAAM
                     RAYANELAQSFGRDGIRANVISPGAVHFPGGSWDRVKEAQPDFYRNVEKAIPLGRLGT
                     GEEIARIVAFVASPAGIWINSAHIAADGGQVAAVD"
     gene            complement(2031..2993)
                     /locus_tag="AC16PP_04860"
     CDS             complement(2031..2993)
                     /locus_tag="AC16PP_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase domain-containing protein"
                     /protein_id="HIR6480989.1"
                     /translation="MTNPVFPDVRMNIVRAADPVTARVVSNELCMRGKSASFVKHLVL
                     DIGDTPLQGAFRAGQSFGVLAPGEDAQGKPHKVRLYSIACPTWGEDGEARLISTTPKR
                     LIDEFSPQTETDDPDDHHLFLGACSNYLCDLKAGDEVRLTGPNGKRFLLPADPDAHDY
                     LLLATGTGIAPFRGMLLELLTRAQGPCRSRIDLVMGAPYATDLLYDDLFRRLADEHDN
                     FRYHTAVSREGADGGRGLYVDDLLRRRLEAFRPLLGGDRTLIYVCGLTGMRISLYRLL
                     VEEGLADGYLTVDASLHDVDPGRWSAVDVRRGIKHSDRCLIEVY"
     gene            complement(2990..3808)
                     /locus_tag="AC16PP_04865"
     CDS             complement(2990..3808)
                     /locus_tag="AC16PP_04865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6480990.1"
                     /translation="MSRIFLLISMLAVAACQPPSDRGGADAPSASVAPAPGAEVGALP
                     SLAERLADRPEADRARDAGRMPAEVVEFLGIEEGMTVLDVFSAGGWYAEVLSHAVGPA
                     GTVYAQNTEFLLSMRDGANEKAMAARLADGRLPNVQRLDRELDDLGLAPGSVDAAMLA
                     LNFHDIYNRGGPDGALAFLGRIYSLLKPGGVLGLIDHHGAAGNDNAALHRVEKAKVLE
                     VIDASDFVLEGESDLLVNPDDDMTSMVFAPEIRGRTSRFLLRLRKPADATAVGP"
     gene            3944..5140
                     /locus_tag="AC16PP_04870"
     CDS             3944..5140
                     /locus_tag="AC16PP_04870"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6480991.1"
                     /translation="MPALDGLRILDMTQYEAGTACTQALAWLGADVVKIEAPGSGDPG
                     RGVWGETSEDSEYFVVWNSNKRSVAIDLRKPEGRDLLLEMLPHYDVFVENYGPGVIER
                     LNLDYDVMRAIHPEIIYARIKGFGLSGPWAEYKCYDMVGQAAASAFSITGEPDGPPMR
                     PGPTTGDAGTGVQMALAITAAYAQKLRTGEGQFIELSMQEAMTYYLRTATASTNYGER
                     PTPRTGNGDLATMSLYPCAGGGPNDYVFLMAVTPRMWGDMCVAIGREDLIEDPRYKRG
                     ADRAANHASLRAEIGRWTLERDKYEAMRILAEGGVPASAVLDTRDLFDNPHLLDRGFV
                     HTVEHEEHGTIRLLGWPARMSRSQVPIKAAPRLGRDTREVIAADLGLAPESVDALVEA
                     GVVAES"
     gene            complement(5142..>5856)
                     /locus_tag="AC16PP_04875"
     CDS             complement(5142..>5856)
                     /locus_tag="AC16PP_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6480992.1"
                     /translation="DALGVEQAVYVGHDWGAPVVWNSAVLHPDKVAGVIGVNNPYIPA
                     IPDDPLNLLRAEWGDEHYIVNFHNKGPDDPDSSDAKFNANVEQVFRGLYRASLFSMAE
                     FERLPKEQRVLRMEPIVFNPDPPGRFLLSDEELAYFVAAFEKTGFTPGLNWYRNISRN
                     WSATRQLDPHIRVPCLMIEVTGDPSGPPGCTELMRPFVEKLDRAVIEDCGHWTQHEKP
                     EELAAIMIAWLDRTFGPEV"
BASE COUNT         1031 a         1991 c         1933 g          901 t
ORIGIN      
        1 ggccggcgag gtcatgccca accccttcgc gctcttgccc gagcgccgtg gccgcgccca
       61 gcctgagcca aaacccgaac cgttcggtta cggcgagcgg ctgagggttc gggatctgac
      121 cgagaacgtc agcggcctgt cggcagcggc cttagcggac gagatcctgc tggaaggcga
      181 aggccaggtg aaggcgctgt tctgcgtcgg cggtaaccct atggcggcct ggcccgacca
      241 gctgaagacc taccaggcca tgaaggcact cgagctcaac gtctgcctgg acatcaagat
      301 gtcggccacc gccaagctcg cccacttcgt gctgccgccc cggctcggcc tggaatcgcc
      361 cgccatctcg ctgccgaatg agaacatctg gttctacggc ttctccaccg ggttccccga
      421 accttacgcc atgtaccagc cccagctcgt cgagccgcca gcgggctccg atctgctgga
      481 ggaatgggag gtcttctacg gcgtcgccca gcgcatgggg cttgggctca ccttcgccgg
      541 ggaggggctg gacatgaacc gcaagcccac cacggacgag ctgttcgaga tcatgtgcaa
      601 aggttcccgg ataccgctcg aggaggtcaa acgatacccc cacggccaca ttttctcgga
      661 tccatccatc accgtgctgc caaaggaaga aggctgcgag gaccgtctgg atgtgggaca
      721 cgagctgatg atgcgggaac tcgccgaggt gcgggccgaa cccctgtcgg gccacggggg
      781 ctacgccggc gagcgggcat tcacccaccg cctcgtcagc cgccggatga acgacgtgta
      841 caactcctcc gggcgcgaca ttccgcggct ggtgcgcaac caccgctaca acccggcgta
      901 catgaatcct gacgacatcg aggccctggg gctgcacgat ggggacgtcg ttgagatcgc
      961 ctcggaccac gcgaagattc tcggcatcgt ggaggcagcg cccgacatac gccccggcgt
     1021 ggtgtccatg gctcacgcct tcggcgacgc ccccgagcac gacgaggacg ttttctacat
     1081 cggctcgaac accggccgcc tcacggacgt ggagcgcaac tacgacccca tcaccggcat
     1141 gccgcgcatg agcgcgattc cggtgaacgt ccggcgcggc gaccagagcc tcgcttcccg
     1201 ctgaggcgac cgcacgacca agggcacgaa caaaggaaaa cgcatggaac tgggactcga
     1261 aggtaagcgc gccatcattc tgggcggaag ccgcggcatc ggctggtaca cggcgagggc
     1321 gctggcggtc gagggctgct cggtggccct ctgcgcgcgc gatgccgacg gcgtgcaggc
     1381 cgcggtggac gccctttccg aagatcctgg cggatcccac ttcggcgcgg cggcggacct
     1441 gaccgacggg gacgggaccc gggccttcgt gcgcgaagcc atcacggccc tcggcgggct
     1501 ggacctgctg atccacaacg ccagcggctt cgaccgcagc ggcgatgagg agggctggca
     1561 gcgcagcttt caggtggacg tgatggccgg cgtgcgggcg gtggaggaag cactgcccgc
     1621 gctcaaggaa agcgacgctg ccagcatcac cttcatcggc tccatggcca gcaagttcta
     1681 tttcggccgg cgctcgtcct acgggcccgc caaggccgcc atgcgcgcct atgccaacga
     1741 gctggcccag agcttcggcc gggacgggat ccgcgccaac gtcatctcgc ccggcgccgt
     1801 gcactttccc ggcggctcgt gggaccgggt aaaggaggca caaccggact tctaccgcaa
     1861 cgtggagaag gccatcccgc tgggaaggct aggcaccggc gaggagatcg cccggatcgt
     1921 cgccttcgtg gcgagccccg ccgggatctg gatcaactcg gcccacatcg ccgccgacgg
     1981 tggtcaggtg gccgccgtgg actgagcccc gtgggatgcg ccttccgccg tcaatagacc
     2041 tcgatgaggc agcgatccga gtgcttgatg ccccggcgaa cgtccaccgc tgaccagcgg
     2101 ccgggatcga cgtcgtgcag agaagcgtcc acggtgagat agccgtctgc cagaccttcc
     2161 tccaccagca agcggtagag gctgatgcgc ataccggtca gcccgcacac gtaaatcagg
     2221 gtgcgatccc cgcccagcag gggccggaac gcctccagac gccgccgcag caggtcgtcc
     2281 acgtagagac cccgaccgcc atcggcgccc tcccggctta ccgccgtgtg atagcggaaa
     2341 ttgtcgtgct cgtccgctaa gcggcgaaac agatcgtcgt agagcagatc ggtggcgtac
     2401 ggcgcgccca tcaccagatc gatgcgcgac cggcagggtc cctgagcgcg ggtcagcaac
     2461 tccagcaaca tcccgcgaaa gggggcgatg cccgtgccag ttgccagcag gaggtaatcg
     2521 tgcgcgtcag gatccgccgg cagcaggaag cgcttgccgt tgggccccgt caggcgcacc
     2581 tcgtcgccgg ctttgaggtc gcagaggtaa ttgctgcacg cgccgagaaa cagatgatgg
     2641 tcgtccggat cgtccgtttc ggtctggggc gagaattcgt cgatgagccg cttcggcgtc
     2701 gtcgatatca ggcgggcttc accgtcctcg ccccaggtcg ggcaggcaat ggagtagagc
     2761 ctgaccttgt ggggcttgcc ctgggcgtcc tcaccgggcg cgagcacgcc aaaggactgc
     2821 ccggcccgaa acgccccctg cagcggcgtg tcgccgatat ccagaaccag gtgcttgacg
     2881 aagctggccg acttgccgcg catgcagagc tcgttggaca ccacacgagc cgtcaccggg
     2941 tctgctgccc gaacgatgtt catccgcacg tccggaaaga cagggttggt catgggccga
     3001 ccgccgtcgc atcagccggc ttgcgcagcc gcagcaggaa acggctggtg cggccgcgaa
     3061 tctccggagc gaagaccatg ctggtcatgt cgtcgtccgg gttgaccagg aggtcgctct
     3121 cgccttccag gacgaaatcg ctggcgtcga tgacctccag caccttcgcc ttctccaccc
     3181 ggtgcagcgc ggcgttgtcg ttgccggccg cgccgtggtg gtcgatgagc ccgagcaccc
     3241 cgccgggctt cagcagcgag tagatccgtc cgaggaaggc gagcgccccg tccgggccgc
     3301 cgcggttgta gatgtcgtga aagttcagcg ccagcatggc cgcatccacg ctcccgggcg
     3361 cgagcccaag gtcgtccagc tcccggtcga gccgctgcac gttgggcagc cggccgtcgg
     3421 ccaaccgcgc cgccatggct ttttcgttcg caccgtcgcg catgctgagc agaaactcgg
     3481 tgttctgggc atataccgtg cctgccggcc cgacggcgtg agacagcacc tcggcatacc
     3541 agcctccggc ggagaacacg tcgagcaccg tcatgccttc ctcgatgccg agaaactcga
     3601 ccacctccgc cggcatgcgt ccggcatccc gggcgcgatc ggcctccggc cggtcggcca
     3661 gacgctccgc gagagacggt aacgcgccga cctcggcgcc gggagcgggc gcaacggacg
     3721 ccgaaggtgc atctgcgccg ccgcgatccg atggcggctg gcacgctgcc acggccagca
     3781 ttgaaatcag caagaagatg cggctcatga actcctccct ctgcctcagt gcgacccagc
     3841 atagcgcgct gcgggctccc cctgtagccc gacggccgct ttcttcattt cggtgctgac
     3901 caccgatatg cttgtcggtg gggaacacac aaggggaaag cccatgccgg ctctggacgg
     3961 tttgcgaatt ctggacatga cccagtacga ggcgggcact gcctgcaccc aggcgctcgc
     4021 ctggctgggc gccgacgtgg tgaagatcga ggcgccaggc tccggcgacc ccggccgcgg
     4081 cgtctgggga gaaacctcgg aagattccga gtacttcgtg gtgtggaaca gcaacaagcg
     4141 cagcgtcgcc atcgacctgc gcaaacccga aggccgcgac ctgctgctcg agatgctgcc
     4201 ccactacgac gtgttcgtcg aaaactacgg tcccggcgtg atcgagcgcc tgaacctgga
     4261 ttacgacgtg atgcgcgcca ttcaccccga gatcatctat gcccgcatca aggggttcgg
     4321 cctcagcggc ccgtgggcgg agtacaagtg ctacgacatg gtgggccagg cggccgccag
     4381 cgcgttttcc atcaccggcg agccggacgg tcctccgatg cggccgggcc ccaccacggg
     4441 cgacgcgggt accggcgttc agatggccct ggccatcacc gccgcctacg cgcagaagct
     4501 gcgcacggga gaaggccagt tcatcgagct gtccatgcag gaggccatga cctactacct
     4561 gcgcacggcc acggccagca cgaactacgg cgagcgccca accccgcgaa cgggcaacgg
     4621 cgatctggca accatgtccc tctacccctg cgccggcggg ggccccaacg actacgtctt
     4681 cctgatggcg gtgacgccaa ggatgtgggg cgacatgtgc gtagccatag gccgcgagga
     4741 tctcatcgag gatccccgct acaaacgcgg cgcggatcgt gcggccaacc acgcatcgct
     4801 gcgcgcagag atcggcaggt ggacgctcga gcgcgacaag tacgaggcca tgcgcatcct
     4861 cgccgagggc ggcgtgcccg caagcgccgt gctcgacacc cgggacctgt tcgacaaccc
     4921 ccatctcctg gaccgcggct tcgtgcacac cgtggagcac gaggagcacg gcaccatccg
     4981 cctgctcggc tggcccgcaa ggatgtccag gagccaggtg cccatcaaag cggcaccgcg
     5041 gctcggcagg gacacccggg aggtcatcgc cgccgacctg ggccttgccc ccgagtcggt
     5101 ggatgccctg gtcgaggccg gcgtggtggc agagtcctag gctaaacctc cgggccgaag
     5161 gtgcggtcga gccaggcgat cattatcgcg gccagctcct cgggtttctc gtgctgggtc
     5221 cagtggccgc agtcctctat gaccgcgcga tccagctttt ccacgaacgg ccgcatcagc
     5281 tccgtgcacc cgggcggtcc ggaggggtcg ccggtcacct cgatcatcag acacggcacc
     5341 cggatgtgcg gatcgagctg ccgcgttgcc gaccagttgc gcgaaatgtt gcggtaccag
     5401 ttcaggcccg gcgtgaagcc cgtcttctcg aacgcggcga cgaaataggc gagctcctcg
     5461 tcgcttaaaa gaaaccggcc gggcgggtcg ggattgaaga cgatgggttc catccggagc
     5521 acgcgctgct ccttgggcag ccgctcgaac tccgccatgc tgaaaaggct cgcccggtag
     5581 agaccgcgaa acacctgctc cacgttggcg ttgaacttgg cgtcggaaga atcgggatcg
     5641 tccggcccct tgttgtggaa attcacgatg tagtgctcgt cgccccattc cgcccgcagc
     5701 aggttcagcg ggtcgtcggg aatggcgggg atgtaggggt tgttgacccc gatgaccccg
     5761 gcgaccttgt ccgggtgaag cacggcggag ttccagacca ccggggcgcc ccagtcatgg
     5821 ccaacgtaaa cggcctgctc gacgccgagg gcgtcg
//
LOCUS       DBNHKV010000151         4973 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_801404, whole genome shotgun sequence.
ACCESSION   DBNHKV010000151 DBNHKV010000000
VERSION     DBNHKV010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_801404"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="AC16PP_04880"
     CDS             complement(<1..1042)
                     /locus_tag="AC16PP_04880"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR6480993.1"
                     /translation="MRKDKGNVTPLFGERRRPPESPRPQVVVLDSADFVDAGELGREL
                     DSGADLDRPRLVLLERNELAVLRPHLEADPNLEFILKPVDQDTLQARIFKLVATSEAF
                     RDRQQQLSRVQDHVDRLAYYDRITDLPNNEFFRRHVDVQIRHARRFTRQLAVLAVDLQ
                     SFERVRRRAGEAGTEALMSLSARRIIEAIRDYDVVGQSPNVLPFGDERTMARMEGDRF
                     LLLLPELRHIEEAASVAERVVAAVEVPIQIGGAAFITRPKVGISVYPTDGDDSTSLIR
                     CAQRALLSSSDLRGGRVHFHCEAMNDVIIDRLINEAALRRAFENGGFHLVYQPKVELS
                     TGRTTGVEALLRL"
     gene            1529..3376
                     /locus_tag="AC16PP_04885"
     CDS             1529..3376
                     /locus_tag="AC16PP_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6480994.1"
                     /translation="MTTSNLLDGFTVGDFQVFPREEKIVGGDSSHHVEPKVMQVLVTL
                     ASNAGHTVSRDQILEEVWSDAVVGDEVLSRAISLLRGYFGDERTSPRYIRTVPRKGYE
                     LIAEVGAPPAKSTGAISRGRAYALVALVAALVVATAAVVRWWPATVDADLTLAVMPLA
                     ISGEASRLGYVADGLADHLITHLTRSPELHVVARRSSFLIRDTDANVRAIGEQLGARY
                     IVEGSLAERGANLLVTMYVVDTELGTNIWTTQLLGPANDLASLQVQAERAINEALRAR
                     LGVKLRTSRSAAGAISEDAYLKYHEARYQWSLRGELRIARAIALLQEAIDLAPDFAEA
                     HMALAQTLAVQPFYTDDPVAERFVKARASAARALELDASLAAEASALEGFLLLRERRW
                     QQAEIQLKKALRLDPDQVNAHYWYSWLLSALGKYDEAMREMQIAWRLNPVSAVLNDRL
                     AVAYLWVNDNAEAAARYQAAADLGYLESTQPESYILFLRRAGRFSEIAELLVRMGYSA
                     AWVEPFVRGLEHPDTRQEGAVAIEAASAAGDVPFELLFGIWVLFEDGDRAFANFDYGL
                     KTKYIEFLWADETEFLRDDARFADLIARLNLSDVIDLDEIRSANQDRAK"
     gene            3373..3942
                     /gene="mobA"
                     /locus_tag="AC16PP_04890"
     CDS             3373..3942
                     /gene="mobA"
                     /locus_tag="AC16PP_04890"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HIR6480995.1"
                     /translation="MSLPPESLTALVLCGGAGRRMSGRDKPLLDLKGRPFVAWIAESV
                     KDRVSQVIISANRNLQAYLEVGEVLPDDGAGQGPLDGIATCARRCRTPFLFVCPGDAP
                     HLDAAVIDRLAEAMEKTDAPVVVASDGEQRQNLHLLMKSDESARILSYLESGRRSVSG
                     WLDEVGAVVVEMPELAGSFKDYDSSDDLA"
     gene            complement(3958..4251)
                     /locus_tag="AC16PP_04895"
     CDS             complement(3958..4251)
                     /locus_tag="AC16PP_04895"
                     /inference="COORDINATES: protein motif:HMM:NF046888.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7009 family protein"
                     /protein_id="HIR6480996.1"
                     /translation="MSRAGLRLRLNGADLSALADGGTVTEPTPFPNGMFECSLTAVAD
                     GIGACLTERGVAVTIPTASLGDLEAGNGLRAELQTSGEALHVLIEKDRRPEGR"
     gene            complement(4272..4745)
                     /gene="bfr"
                     /locus_tag="AC16PP_04900"
     CDS             complement(4272..4745)
                     /gene="bfr"
                     /locus_tag="AC16PP_04900"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HIR6480997.1"
                     /translation="MKPIDSDVIVHLNQVLRNELTAINQYFLHAKMFEDWGMHRIAAH
                     EKEESIDEMKHADALMERILFLEGLPNLQDLGKLYIGQDVKEILECDLKLELEAIPML
                     REAIAHCEHVGDYVSRQLFRDILSSEEEHVDWIETQLDLIAKTGVENYIQSQMQG"
BASE COUNT          940 a         1571 c         1518 g          944 t
ORIGIN      
        1 ccaggcgcag cagagcttcc acgccggtgg tgcggccggt cgagagttcc accttcggct
       61 ggtacaccag gtggaatccg ccgttttcga atgctcgacg cagcgccgcc tcgttgatca
      121 gccggtcgat gatcacgtcg ttcatggctt cgcagtggaa atggactcgg ccaccgcgca
      181 aatcgctcga tgacagcaac gcgcgctggg cacagcggat gagcgatgtg gagtcgtcgc
      241 cgtccgtcgg gtaaacgctg atcccgacct tcggtcgcgt gatgaacgcc gcgccgccga
      301 tctggatagg cacttccaca gcggcaacca cccgttctgc gacgctggcg gcttcctcga
      361 tgtggcgcag ttccggcagc agcagcagaa agcgatcgcc ttccatgcgc gccatggtgc
      421 gctcgtctcc gaatgggagc acgttgggag actgacccac aacgtcgtag tcccggatag
      481 cctcgatgat gcgacgggca gacagggaca tcagggcctc ggtacccgct tcaccggcgc
      541 gtcgcctgac tcgctcgaag ctctgcagat ccaccgcaag caccgccagc tgtcgcgtaa
      601 aacgccgcgc gtggcgaatc tgcacatcca cgtgacggcg gaaaaactcg ttgttcggca
      661 ggtcggtaat cctgtcgtag taggccagcc ggtccacgtg atcctgcacc cggctcaact
      721 gctgctggcg gtccctgaag gcttcggagg tggccaccag cttgaatatc ctagcctgca
      781 aggtgtcctg gtctaccggc ttgaggatga attccagatt cgggtccgct tccagatggg
      841 gacgcagaac ggccagttcg tttcgctcga gcaataccag acgcgggcgg tcgaggtccg
      901 cgccgctgtc gagttcccgg ccgagttcgc ccgcatccac aaagtcggca ctgtccaaca
      961 cgaccacctg gggcctgggc gattccggtg gacgccgcct ctccccgaac agcggagtca
     1021 cgttgccttt atcttttctc atccagtgct cactatcgca gacccggtgc ctgccgcgac
     1081 cccctcccgt tcgccgcggc aacaagcacc gaattctgct ttccccccaa ctcgaatcca
     1141 cgcagatgat cgagtggctt ttctataatc ttcagcgagg tcatcccgcc gagtgtctgg
     1201 cccgatcagc cttcctgcag cgcagctaga ctgacgacat ttggcaattt cgttgccata
     1261 acggccgttt tgtaaaccct acccaattac ctgttagggt gataacagcg cggtgtaagc
     1321 gacatgtaac ctcggggtga tcgcaggggc gctggcgtga caacaacgcg gatgcgcgca
     1381 gcccccgagg acggtatctt caggctatga accagcacga gtccctaaag ccgggcaacg
     1441 ttggcagatg cagccggcgt cgctgcccgc acgccccgca caccaacggc agtcgtcgat
     1501 cgacctcctc gtccatggac gccgcccaat gacaacaagc aatctgctcg acggttttac
     1561 cgtcggcgac ttccaggttt ttccaaggga ggagaaaatc gtcggcggcg attccagcca
     1621 ccatgtcgag ccgaaagtca tgcaggtgct cgtgacgctg gcgtccaatg ccggccacac
     1681 cgtatcccgt gaccagatct tagaggaagt ctggtcggac gccgtggtcg gcgacgaggt
     1741 gctttcccgg gccatatcgt tgctgcgggg ctacttcggc gacgagagaa cgagcccgcg
     1801 ttacatccgt acggtcccac gcaaaggtta cgagctgatt gccgaagtgg gcgcgccccc
     1861 agcgaagagc acgggcgcga tcagccgggg ccgagcgtac gcgttggtgg ccctggtagc
     1921 cgcactggtg gtcgccaccg cagccgtcgt gcgctggtgg ccggccacgg ttgacgcgga
     1981 cctcaccctg gccgtgatgc ctctggccat ctcaggggaa gcgagtcgat tggggtacgt
     2041 ggctgacgga ctggccgacc acctgatcac tcacttgacc cgatccccag agctccacgt
     2101 cgtcgcgcgt cgatcgtcct tcctgatcag ggacaccgac gcaaacgtcc gcgccatcgg
     2161 cgagcagctt ggcgcccgct acatcgttga aggctctctg gccgagcgtg gggccaatct
     2221 gctggtgacg atgtacgtgg tggataccga actcggcacc aacatctgga ccacgcagct
     2281 gctcggcccg gcgaacgatc tggcgtcgct gcaggtccag gccgagcggg ccatcaacga
     2341 ggctctgcgg gcccgactcg gcgtgaagct tcgaacgagc cggtcggcgg ccggcgcgat
     2401 ctccgaggac gcctatctga aatatcacga agctcgctac caatggagcc tgcggggcga
     2461 gcttcgaata gccagggcaa tcgccctgct ccaggaggca atcgacctgg cccccgattt
     2521 cgccgaggcg cacatggccc tggcccagac cctggcggta cagcctttct acaccgacga
     2581 cccggtggct gagcggttcg tgaaggcccg ggcgagcgca gcaagggcgc tcgagctgga
     2641 cgcctccctg gcggcagaag cctcggcgct ggaagggttc ctgctcttgc gcgaacgacg
     2701 ctggcagcaa gccgagattc agctgaagaa agcgcttcgc ctcgacccgg atcaggtcaa
     2761 tgcccactac tggtactcct ggctgctgtc cgcgctcggc aagtacgatg aagcgatgcg
     2821 cgaaatgcag atcgcatggc gtttgaatcc cgtatcggcc gtgctaaacg accgactggc
     2881 cgttgcgtac ctatgggtca acgacaacgc ggaagcagcc gctcgatatc aggccgccgc
     2941 cgatctcggc tacctggaga gcacccagcc tgagagctac atcctgtttc ttcgtcgagc
     3001 cggccgcttc agcgaaatcg ccgaactgct tgtgcgaatg ggatactcgg cagcgtgggt
     3061 ggagccgttc gttcgcgggc tcgaacatcc cgatacccga caggaaggcg cggtggccat
     3121 agaggccgcc agcgcggcgg gcgacgtacc cttcgagctt ctcttcggca tctgggtgct
     3181 gttcgaagat ggcgaccggg cattcgcaaa cttcgactac ggactaaaga cgaagtacat
     3241 cgagtttctc tgggcggacg aaaccgagtt tcttcgcgac gatgcccgct ttgccgacct
     3301 gatcgcccgc ctgaacctct ccgacgtgat cgacctcgac gagattcgaa gcgcgaacca
     3361 agatcgcgca aagtgagcct gcccccggaa tcgctgacgg ccctcgttct ttgcggcggc
     3421 gcggggcgtc ggatgagcgg gcgcgacaag ccgctcctgg acctcaaggg caggcccttc
     3481 gtggcgtgga ttgccgagag cgtcaaagat cgcgtgagcc aggtgatcat cagcgccaac
     3541 agaaatctgc aagcctacct cgaagtcggg gaagtgctcc cagacgacgg cgcgggtcag
     3601 ggccctctcg acggcatagc cacctgcgcc cggcgatgcc gaactccgtt ccttttcgtg
     3661 tgtcctggcg atgcgccgca tctggatgcc gccgtgatcg atcggctcgc ggaggcgatg
     3721 gagaagacgg acgctcctgt ggttgtggca agcgacggcg agcagcgcca gaacctccac
     3781 ctgctcatga agtcggatga gagcgcgcgc attctaagct acctggaatc tggccgtcgc
     3841 agcgtgtcgg gctggcttga tgaggtcggc gccgtcgttg tggagatgcc cgaactcgcc
     3901 gggtctttca aggattacga ttcgagcgac gacctcgcct gaccgggcag gagcgcctca
     3961 gcggccctcg ggccggcggt ctttctcgat gagcacgtgg agcgcctcgc cggacgtttg
     4021 cagctccgcc ctcaagccgt tcccggcttc cagatcacca agcgaggccg tcggtatcgt
     4081 cacggcaacg ccgcgctcgg tcaggcacgc gccgatgccg tccgccaccg ccgtcaggct
     4141 gcactcgaac atgccgttgg gaaacggtgt tggctccgtc accgtccccc cgtcagcgag
     4201 tgcggacagg tcggcgccgt tgagccgaag acgaaggccc gctctgctca accggagctt
     4261 caccgatgat ctcagccctg catctgggac tggatgtaat tctcgacgcc ggtcttggcg
     4321 atcaggtcga gctgggtttc gatccagtct acgtgctctt cctccgatga caggatgtcg
     4381 cgaaagagct gtcggctgac gtagtcgcca acgtgctcgc agtgagcgat ggcctcacgc
     4441 agcatgggaa tcgcttcgag ttcgagtttt aggtcgcact cgaggatctc cttcacgtcc
     4501 tgaccgatgt agagcttgcc gaggtcctgc aggttcggca gcccttccag gaacaggatg
     4561 cgctccatca gcgcatcggc atgcttcatc tcgtctatgg actcctcttt ctcatgagcg
     4621 gcgatccgat gcatccccca gtcttcgaac atctttgcgt ggagaaagta ttgattgatg
     4681 gccgtgagct cgtttctcaa tacctgattg aggtgaacga tgacgtcgct gtcgatgggc
     4741 ttcatggaat gatcctgaac cggttcgaaa gacggactat gcgggagcga gtcggactta
     4801 gcaaggccgc ctctctctaa gtgactgatt tatattgaaa agcattccca tctgaaaata
     4861 ccatccggtt acaaacgaga accaaaaact attgcaaccg agaatgattt gcatttaaat
     4921 caacggcagt tcatccgatc gctcacttga gatgtacgtc tgcctctgta aag
//
LOCUS       DBNHKV010000152         5779 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1792373, whole genome shotgun sequence.
ACCESSION   DBNHKV010000152 DBNHKV010000000
VERSION     DBNHKV010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1792373"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="AC16PP_04905"
     CDS             complement(<1..1631)
                     /locus_tag="AC16PP_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR6480998.1"
                     /translation="MAFEPGTPASEIAAAHRRNGAQIQQSIDSIGVQVLSIPSGSVPS
                     LLQVYRNNPNVKYAEPNYLRPLILPGEGSFGNNIDVISEQWSLHNQGQTLHTLVDPVT
                     GVSPYPYTAPGADISMDEAWVIEKGDPDVLVAVPDSGVDCQHGDLFMKCAHNEDYVTP
                     TVDPYGTPIPELVDRIGHGTHVAGTIGMDTDNDVGGAGIGWNVRVGSFKVCYQVEYVG
                     IVIGSACQDADIAAAIARAIELGYHVINMSFGQAAPSAVLKAALDDAVDAGLILVAAA
                     GNNGNWQQFYPAAYPEVISVGATTSWDDRAGFSTFSRDEDDWVDILAPGDSILATVPN
                     VFCSSTATQCFGWKRGTSMASPHAAGVAALVLSYLQRTDAANANRLEVRRRMQDCADK
                     TGAMGQDMLVWSRYGRLNAAAALTCGDTGGGGGGGGGGGGSDPTGSHIADLTSSSTSA
                     GGNAWSSTVTVRVHDASHGSLSGVTVNFVTDRSTDPTRSCTTGTVGSCTTEPVLQHKK
                     NNSVTYTISSLSAPDYTPGDNHDGSGSPIGDSVTA"
     gene            complement(2043..2252)
                     /locus_tag="AC16PP_04910"
     CDS             complement(2043..2252)
                     /locus_tag="AC16PP_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6480999.1"
                     /translation="MRTRVQIEDGDVSITFEPESEVERLCITELGDEISVSRSHKNIV
                     LRRRRDNVRKISDLEPVGQLAEESL"
     gene            2483..3832
                     /locus_tag="AC16PP_04915"
     CDS             2483..3832
                     /locus_tag="AC16PP_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR6481000.1"
                     /translation="MLYRAFTSRPGSLAALAAMMAALLAPSSHAADNGLVVYLSAYLS
                     GDYYYAPVPDLLPNESQQEHIQPIQLSLPRDFRRSVELGNHDISADGQIIVFAARTKR
                     RQDWDIYTGTPDYDQAEITNVQRIIHESGVREEDPRFDWTRDSSGLHRVVYKCDGDIC
                     IYDGAITKVVAEADCELWAPSFNSEGTAVSYVRRCDGRDSDRIWYAYLNAPYDREEVP
                     RPGNAGGPDRFSHFLDSDSLVYSHIMPDTGAAALWRYDGSSGSLTQLTFNTNTNSDDD
                     AYPDKSNPGRIAYIGWQEGTGYNLFIYENQQSYKLSNGVPMLAPAIFRPAGSTEPPPE
                     PPTEPPGTSAVHVGDLDVATGAGKRHWWSRVTVALHDGDENPAAGVTVALNTDSDSVS
                     CVTDEAGRCTTKRLNQNNSVASVEYTVVSVSAPEYNAAANHDPDGDSDGTSITAHQP"
     gene            complement(3937..5349)
                     /locus_tag="AC16PP_04920"
     CDS             complement(3937..5349)
                     /locus_tag="AC16PP_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HIR6481001.1"
                     /translation="MLYEPGRNIPATHHIDTTSFYSTASAARYAGAATVDVVGPDLQL
                     RKTVMAAGGTCGVDDATSIEVIYGDSVEYCFAMENAGDVPLTQVTLDDAKIELTIQPN
                     GGIMAAAETTVFKSDPVAMYVTDTNTAVANALEPLTGTKIGPEQSSATVNVLAADVSV
                     DKRIDTTTIVVCDAGDINPYCSEVNAQGLYDGVYSIVVSNAGPTDAWDVTVTDTLPDG
                     VKVISADSRCDISGAPLISCYLGDMKSATSETLYLSVEIDPGVFEFPWGTITNTACAN
                     TNPAQLDPDAKGKLKSSMITAEVPANINAYVDEHAISPIDIMTRGLLGANVSSWSDGT
                     PRSALGQARTKTSRQLIAAWCNEQVLGSKFAKHFLSWEKIRAIMVGEAYLSAGVVVGC
                     DGPCETRVRDVIDSITELNRVADEFNNSGLDLEDGLPQEPANTKAPKDDPTDPGVESP
                     ISTATSLDASTNKGKGNGRK"
     gene            complement(5485..>5779)
                     /locus_tag="AC16PP_04925"
     CDS             complement(5485..>5779)
                     /locus_tag="AC16PP_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481002.1"
                     /translation="ILEHLRELGCDAAQGNYISPAVDVASFSQWLQNSQWKPRRTNPR
                     QSTSETSGWPGRAKPRAVAHTDLESIESAIQDLEEFVRQVDPKPDGSKAAGAS"
BASE COUNT         1054 a         1816 c         1824 g         1085 t
ORIGIN      
        1 atcgcggtaa cggagtcgcc gatgggtgaa cccgagccgt cgtgattgtc accgggggtg
       61 taatccggcg cgctcagcga gctgatggtg tacgtcacgc tgttgttctt cttgtgctgg
      121 agcaccggct cagtcgtgca ggacccgact gtgcctgtgg tacacgaccg cgtggggtcg
      181 gtgctcctgt ccgtcacgaa gttcaccgtc accccggaca acgatccgtg gctggcatcg
      241 tgaacgcgca cggtgacggt ggatgaccag gcattgccgc ccgccgacgt gctggaggaa
      301 gtcaggtccg caatgtggct tcccgtcgga tccgagccac cgccgccacc accgccgcca
      361 ccgccgcctc cagtatcccc gcaggtgagc gcggctgccg cgttgagccg gccgtagcgg
      421 ctccagacga gcatgtcctg ccccatggcg ccggttttat ccgcgcagtc ctgcatgcgg
      481 cgccggacct ccaggcggtt ggcgttggca gcgtcggttc tctggagata gctcagcacc
      541 agagcggcaa ctccggccgc gtgaggcgac gccatggaag tcccgcgctt ccagccgaag
      601 cactgcgtgg ccgtggacga gcagaacacg ttgggaaccg tggcgaggat ggaatcaccg
      661 ggcgcaagga tgtcgaccca gtcatcttca tctctgctga acgtggaaaa cccggcccgg
      721 tcgtcccatg acgtggtcgc gcccacggag atcacttccg gataggcggc cggatagaac
      781 tgctgccagt tgccgttgtt gcccgcggca gccaccagga tgaggccagc gtccacggcg
      841 tcgtccagcg cggctttgag aaccgccgaa ggggcggcct gaccgaagct catgttgatg
      901 acgtgataac cgagctcaat tgcccgggcg atggcagcgg cgatatccgc gtcctgacag
      961 gcggacccga tcacgatgcc cacgtactct acctgatagc agaccttgaa cgagccgacg
     1021 cgaacgttcc agccgatgcc tgcaccgccg acgtcgttgt cggtatccat cccgatggtg
     1081 cccgccacgt gggtgccgtg accgatgcgg tcaaccagct ccggaatcgg cgtgccgtaa
     1141 ggatcaacgg tcggcgtcac gtagtcttca ttgtgggcgc acttcatgaa cagatcgcca
     1201 tgctgacagt ccacgccgct gtccggcacg gcgacgagca cgtcgggatc gcccttctcg
     1261 atgacccatg cctcgtccat gctgatgtcg gcgccgggag ccgtgtaggg gtaaggggac
     1321 acacccgtta ccggatcgac gagcgtgtgc aacgtctggc cctggttgtg caggctccat
     1381 tgctcactga ttacgtcgat gttgttgccg aacgaacctt cccccggaag tatcagcgga
     1441 cgcagatagt ttggctcagc gtatttcacg ttcggattat tgcggtagac ctggagcaag
     1501 ctcggcacgc tgcccgacgg aatggataaa acctggacgc cgatggagtc gatggattgc
     1561 tgaatctgcg ccccgttacg gcgatgggca gcggcgatct cggacgcggg cgttcccggt
     1621 tcaaatgcca ccagcagctc atcatccaca taggccggta ccgccggcgt cgacgcagtc
     1681 gaagccgccg cgctcgtgac caggaggacg gccgctgcag ccagaacacc gagtcgcttg
     1741 cctggccttg gaaaaagatg gtacatcgta agtgtcgctc cccagcatga gggcgggaac
     1801 tacggggcaa acgcacccgt cgcgtctcgc cattgcaaag taattcgatc acccgatgtt
     1861 agaggtcggt gggtgctgag gaggtgaccg gtttctcagc ggtttgtaaa cccggggcta
     1921 caactcgcgt atccggtcga gcccgtgccc tggtgttgct tctctgcaga agcgcggccg
     1981 ccgggcggta acgggcagaa caacggggcg cttccatgca ccgccttgtt ccgggttggc
     2041 tgctaaagcg actcctcagc cagctgccct accggctcga gatcgctgat cttgcggacg
     2101 ttgtcgcgac ggcgccgcag cacgatgttc ttgtggctgc gggagacgct gatctcgtca
     2161 ccgagctcgg tgatgcacag gcgttccacc tcggactccg gctcgaaggt gatgctgacg
     2221 tcgccgtcct cgatctgcac tctggttctc atgtctttgt cctctggggt tgtggatcgg
     2281 acagatgcat tatccgaacg ctccccatgc cctggtatgg gcgacatgtc agcggggttt
     2341 gctggaattt ttggcgagaa cgcgttttga gacccgcgtc atggaaaatt acggcgtaga
     2401 gatagccctt gaattcgtca agataatttg cataatggcc ggatggtagt tgatcggccg
     2461 ggggggccga cggggggaca ccatgttgta ccgcgctttc acatcccggc ctggttcact
     2521 cgcggctctg gctgccatga tggctgcgct gctggcgccg tcgtcacacg cggccgataa
     2581 cggcctcgta gtctacctga gcgcttacct gtcgggtgac tactactacg cgccggtacc
     2641 cgatctcctg ccgaacgagt cccaacagga acacatccag ccgatacagc tcagcctgcc
     2701 ccgggatttc cgccgttccg tggagctcgg caatcacgac atctccgccg acggccagat
     2761 catcgtgttc gccgcgcgca cgaagcgaag acaggactgg gacatctaca cgggcacgcc
     2821 cgactacgac caggctgaaa tcaccaacgt tcagcggatc atccacgagt ccggcgtgcg
     2881 cgaggaggac ccgcgcttcg actggacgcg cgactccagc ggcctgcacc gtgtcgttta
     2941 caaatgcgat ggcgacatct gcatttacga cggtgccatc accaaagtcg tggcggaagc
     3001 cgattgcgaa ctctgggccc catctttcaa ctcggagggc accgccgttt cctacgtgag
     3061 gcgttgcgat gggcgggata gcgaccgcat ctggtacgcc tacctgaacg ccccgtacga
     3121 ccgggaggaa gtaccccgcc caggcaatgc gggcggcccg gaccggtttt cccacttcct
     3181 ggacagcgac agcctcgtct actcacacat catgcctgat accggcgctg cggcactgtg
     3241 gcgctatgac ggttccagcg gctccctgac gcagctcact ttcaacacga acaccaactc
     3301 cgacgacgac gcctatccgg acaagtccaa ccccggccgc atcgcctaca tcggctggca
     3361 ggagggcacg ggctacaacc tgttcatcta tgagaaccag cagtcataca agctctcgaa
     3421 cggcgtgccc atgctggcgc cggcgatttt ccgacccgcc ggctccacgg aaccgccgcc
     3481 cgaaccgccg acggagccac ctgggacctc tgcagtccac gtgggcgacc tcgatgttgc
     3541 caccggggcc ggcaagcggc actggtggtc gcgggtcacg gtagcgctgc acgacgggga
     3601 cgagaatccc gcggccggcg tcaccgtcgc gttgaatacg gactccgaca gcgtcagctg
     3661 cgtcaccgat gaggcgggga gatgcacgac caagcgtctg aaccagaaca actcggtggc
     3721 atccgtggaa tacacggtgg tttcggtttc cgccccggaa tacaacgcgg cggcgaacca
     3781 cgacccggac ggcgatagcg acggaacgag catcaccgcg catcaaccct gaccggccag
     3841 cccgggcggg gctcgctccc caccgacacc cgtcaacccg gcaaaaaaaa gaccccgccg
     3901 aagcggggtc tttggtttgg aggcagagcc ggtcgcttac ttgcggccgt tgcccttgcc
     3961 tttattggtt gaggcgtcga ggctcgtcgc ggtggagatc ggcgactcga cacccggatc
     4021 cgtcgggtca tcctttggcg ctttcgtgtt cgccggctcc tgaggcaatc catcctccag
     4081 gtcgagtccg gaattgttga actcgtcggc cacccggttc agctcggtga tcgagtcgat
     4141 cacgtccctg acccgggtct cgcaaggtcc atcgcaaccg acgaccactc ccgcgctcag
     4201 gtaggcttcg ccgaccatga tcgcgcggat cttctcccag ctgaggaagt gcttggcaaa
     4261 cttgctgccg agaacctgct cgttacacca ggctgcaatg agctgacggg aggtcttggt
     4321 ccgagcctga ccgagggcag accggggggt gccgtcgctc caggaggaaa cgttggcgcc
     4381 gagcaggccc cgcgtcatga tgtcgatggg agaaatggca tgctcgtcca catatgcgtt
     4441 gatgttcgcc gggacctccg ccgttatcat gctcgacttg agcttgccct tcgcgtccgg
     4501 atcgagctgc gccgggttcg tgttggcaca ggccgtattg gtgatggttc cccaggggaa
     4561 ctcaaacacg ccgggatcga tctccacgga caggtagagc gtttccgaag tagccgactt
     4621 catgtcaccc aggtagcagc tgatcagcgg ggcaccgctg atatcgcagc gggagtcggc
     4681 gctgatcacc ttgacgccgt cgggcagcgt gtcggtgacg gtcacgtccc aggcgtccgt
     4741 cgggccagcg ttgctgacaa cgatcgagta gacgccgtcg tagagaccct gggcattgac
     4801 ctcgctgcag tagggattga tgtcaccggc atcgcagacc acgatcgtcg tcgtatctat
     4861 ccgcttgtcg acagacacgt ccgctgccag cacgttgacg gtggccgagc tctgctcggg
     4921 gccgatcttg gtacctgtga gcggctctaa cgcgttcgca accgcggtat tggtatccgt
     4981 tacgtacatg gcaaccggat cggacttgaa gaccgtagtt tctgccgcgg ccatgatgcc
     5041 gccgttcggt tggatggtga gctcgatctt cgcgtcatcc agcgtgacct gggtcagcgg
     5101 gacgtcgcct gcattctcca tggcaaagca gtactccacg gaatcgccgt agatgacctc
     5161 gatgctggtg gcgtcgtcaa caccgcaggt gccgccggcc gccatcaccg tcttgcgaag
     5221 ctgcaggtcc gggccgacca catcgacggt ggctgctcca gcgtagcgag cggcagaggc
     5281 cgtgctgtaa aagctcgtgg tgtcgatgtg atgcgttgcc gggatattgc gacctggttc
     5341 gtacaacaac cggaaactgg cgcacaacgt gcaggggggt agttaacagc tgcgccgaga
     5401 accggcgcag aggcggacaa atcccttaca tcggggaaaa catatagaca aacggactat
     5461 cggccaggcc gatgacggtg cgtgtcagct ggcgcctgcc gccttggagc cgtcaggctt
     5521 gggatcgacc tggcgcacga attcctccag gtcctggatg gcggattcga tggattcgag
     5581 gtccgtatgg gccaccgctc tgggcttggc acggcccggc caaccggaag tctccgacgt
     5641 cgattgacgg ggattggtcc ggcggggctt ccattgggag ttctggagcc actgggagaa
     5701 gctcgcgacg tccacggccg ggctgatgta gttgccttgg gcagcatcac accccagctc
     5761 gcgcagatgc tccagtatc
//
LOCUS       DBNHKV010000153         2711 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1580604, whole genome shotgun sequence.
ACCESSION   DBNHKV010000153 DBNHKV010000000
VERSION     DBNHKV010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1580604"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="AC16PP_04930"
     CDS             complement(<1..586)
                     /locus_tag="AC16PP_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6481003.1"
                     /translation="MTYKLGVDVGGTFTDVLLIDSESGAVHTAKVPSTPEDSSVGVLN
                     GIARACANAGVDPAAITQVTHGTTVATNAVLTGRGALVGLVTTRGYRQVLQIARSYVP
                     GGLGGWVIYNKSDPMAPLECTVEVPERIGADGSVVEALDEDAARSALRSLGARGIEAL
                     TVSLINSFTNPAHEERLAEIAAEELPGVPVSLSSE"
     gene            complement(589..1494)
                     /locus_tag="AC16PP_04935"
     CDS             complement(589..1494)
                     /locus_tag="AC16PP_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HIR6481004.1"
                     /translation="MTETVIINDVGPRDGLQNDAADVTPEDRALLINRLIDAGVPAVE
                     VASFVSPRAVPKMAGADRVVAGIDLSRADCSALVPNHKGYELARDAGIRSVAVVLSAT
                     ETMNLKNINMDLETTVRVCEEVVGRAVQDGIHARAYVSVAVECPYEGLVSEETVASLA
                     GRMFDAGAAEVIVADTIGAANPAQVKSLFEKLIGAFGAERLSAHFHDTRALALANAWQ
                     ALECGIRKFDSSIGGLGGCPFAPGAAGNLATEDLASMLEQAGFHTGIDMDSLLSVVAL
                     ARELVGHDLGGRSLAWQLGRKTEAA"
     gene            complement(1527..>2711)
                     /locus_tag="AC16PP_04940"
     CDS             complement(1527..>2711)
                     /locus_tag="AC16PP_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6481005.1"
                     /translation="GIRLLDLTHMLSGPYAGMILADLGCDTVKVEPPGTGEGTRRLLE
                     TDPKNSFKGMGAYFFTLNRNKRSVAIDLKSETGRRYFYELVKVADIVLDNFSADVTKR
                     LLIDHDSLAAVNPRIVTCSVSGFGEDGPHYRRPAFDQIVQGLGGGMSITGSGPGEAVR
                     AGIPIGDLGGGMFAVMGVQAALLARERTGVGQHVDISMLDVQISLLNYMATMYTLSGE
                     VPGPIGNSHFVHTPYNTFETADDTIIIAVIGDTFWPALCDLLEIDELRKPEFATAPKR
                     LEHKAYIEGLIGERLLTRPAAEWLEALEAARIPCARVNNIAQALDDPQILHRHMVVEV
                     EHPDGGRVKVPGNPIKLSGTPAETFSPPPLLGAHTREVFAEWAGMAEARIAEGMEAGA
                     IA"
BASE COUNT          468 a          908 c          863 g          472 t
ORIGIN      
        1 cttccgatga cagagaaaca ggcacgccgg ggagctcttc cgcggcgatc tccgctaggc
       61 gctcctcgtg ggccgggttg gtaaagctgt tgatgagcga tacggtcaat gcctcgatgc
      121 cgcgggcgcc cagggaacga agcgccgatc tggcagcgtc ttcgtcgagc gcttcgacta
      181 ccgagccgtc agcgccgatg cgctcgggga cctccaccgt gcactccagc ggcgccatgg
      241 gatccgactt gttgtagatc acccagccgc ccaagccgcc gggcacgtag gatcgggcaa
      301 tctgcaggac ctgccggtac ccgcgcgtgg tcacgagacc gaccagggcg ccacggccgg
      361 tgaggaccgc attggttgcc acggtggtgc cgtgggtgac ctgggtgatc gccgccggat
      421 ccacgcccgc gttggcgcac gccctggcaa tgccgttgag cacgcccaca gatgagtcct
      481 ccggcgtcga aggaaccttg gccgtatgca cggcgccgct ctcggaatcg atgagcaaca
      541 cgtccgtgaa cgttccaccg acatctacgc ccagcttgta tgtcatggtc aggccgcctc
      601 cgtctttctg cccagctgcc aggcaagcga gcggccaccc aggtcatgac ccaccagctc
      661 gcgcgccagc gcgaccaccg agagcaacga gtccatgtcg attcccgtat ggaagccggc
      721 ctgctcgagc atggaggcca gatcttccgt ggccagattg ccagcagcgc ccggcgcgaa
      781 agggcagccc cccagcccgc ctatgctgga atcgaacttg cggatcccgc attccagcgc
      841 ctgccaggcg tttgccaatg ccagcgctcg ggtgtcgtga aagtgggccg agagccgttc
      901 agcaccgaag gcgccgatga gcttttcaaa aagagacttc acctgggccg gattcgccgc
      961 gccgatggtg tccgccacga tcacttcggc cgcccccgcg tcgaacatgc gcccggcaag
     1021 ggacgctact gtttcttctg agacgagacc ctcgtaaggg cactccactg ccaccgatac
     1081 gtaagcacga gcgtggatac cgtcctgaac agcccggccg accacctcct cgcagacgcg
     1141 caccgtggtc tccagatcca tgttgatgtt cttcagattc atggtctcgg tggccgagag
     1201 caccacggca acggagcgga tcccggcgtc acgggccagc tcgtagccct tgtgattagg
     1261 caccagcgcg ctgcaatcgg cgcgagagag atcaatgccg gcaacgaccc gatccgcgcc
     1321 ggccatcttc ggcacggccc gcgggctgac gaagctcgcc acctccaccg cgggcacgcc
     1381 ggcgtcgatc agtcggttga tgagcaacgc tcggtcctcg ggcgttacgt ccgcagcgtc
     1441 gttctgcaga ccgtcccggg gcccgacgtc gttgatgatt acggtttcgg tcatctttcc
     1501 aatcctcgtc cagcccgctg cgcgggtcag gcaatcgcgc cggcttccat tccttcggcg
     1561 atccgggctt cggccatccc cgcccactcg gcaaagactt cccgggtgtg ggctcccagc
     1621 agcggtggcg gcgagaacgt ctccgccggc gtgccggaga gcttgatggg attgccgggc
     1681 actttgacgc gcccgccatc cggatgctcc acctccacga ccatgtgccg atggaggatc
     1741 tgggggtcat ccagagcctg cgcgatgttg tttacccgcg cgcaggggat gcgcgccgct
     1801 tccagggctt ccagccactc cgccgccgga cgggtaagga gcctttcacc gatgagaccc
     1861 tcgatgtacg ccttgtgctc gagccgcttg ggcgccgtgg cgaactcagg cttgcgcagc
     1921 tcatcgatct ccagcaggtc gcacagggcc ggccagaagg tgtcaccgat caccgcgatg
     1981 atgatcgtgt cgtccgcggt ttcgaaggtg ttgtacgggg tgtgcacgaa gtggctgttg
     2041 ccgatgggcc cgggcacctc gccggacagg gtgtacatgg tcgccatgta gttgagcagg
     2101 ctgatctgca catcgagcat ggagatgtcc acgtgctggc caacgcccgt gcgctctcgc
     2161 gccagaagcg cagcctgcac gcccatgacc gcgaacatcc caccgccgag gtcgccgatg
     2221 gggatgccgg cacggaccgc ctcaccgggg ccgctgccag tgatggacat gcccccgccg
     2281 agcccctgga cgatctgatc gaaagccggc cgccggtagt gggggccgtc ttcgccgaac
     2341 ccggaaaccg agcaggtcac gatgcgggga ttcaccgccg ccaggctgtc atgatcaatg
     2401 agcagtcgct tggtgacatc ggcggagaag ttgtccagaa cgatgtcagc gaccttgacc
     2461 agctcgtaga agtaccggcg acccgtttcc gatttgaggt cgatggccac gcttcgcttg
     2521 ttgcggttga gcgtgaagaa gtacgcgccc atccctttga acgagttctt gggatcggtt
     2581 tcgagcaggc gccgggtgcc ctcgccggta cccggcggct ccaccttcac ggtgtcgcag
     2641 ccgagatcgg caaggatcat gcctgcatat ggacccgaca gcatgtgtgt caggtccagc
     2701 aggcgaatgc c
//
LOCUS       DBNHKV010000154         2434 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_374868, whole genome shotgun sequence.
ACCESSION   DBNHKV010000154 DBNHKV010000000
VERSION     DBNHKV010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_374868"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="AC16PP_04945"
     CDS             <1..1294
                     /locus_tag="AC16PP_04945"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR6481006.1"
                     /translation="VDFELQHAAMERATRLLLDIVGGEPGPVVEAVEPKALPEVSDIG
                     LRERRLNRLLGTEIAPEIVDEAMERLDFAVTSRETGEEGVRWTVRVPSHRFDIVREAD
                     LVEEVCRIYGYNNVPVRQPVTELELTPVPLERSPAGRLKRLLATLGYQEAITYSFVDP
                     RLQDLLAPAADALALSNPMSSEQSVMRLNLLPGLVEALRFNVARQQDRVRIFELGSCF
                     APGDPVLQKLMLGGVLWGRRHGENWNQSGAEVDFYDLKGDVEAIVGWSGEPVAYERLE
                     DPVLHPGQAALVVGSRGPVGRLGRLHPEIERALDLPGHVFIFELEADAVLHRARRRHL
                     SLSKFPSVRRDLAVVVQADTASSSIERILRESLGEILVDFRIFDVYQGKGIDSNEKSL
                     GIGLTLQHPSATLNDQEIGKYMNDAVDALVRGVGARLR"
     gene            1358..1663
                     /gene="ihfA"
                     /locus_tag="AC16PP_04950"
     CDS             1358..1663
                     /gene="ihfA"
                     /locus_tag="AC16PP_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HIR6481007.1"
                     /translation="MGALTKAQMADRLFDELGLNKREAKEIVEMFFDEVRDSLENNQQ
                     VKLSGFGNFDLREKGERPGRNPKTGEEIPITARRVVTFRPGQKLKARVEAYAGPGSD"
     gene            1641..1997
                     /locus_tag="AC16PP_04955"
     CDS             1641..1997
                     /locus_tag="AC16PP_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6481008.1"
                     /translation="MLDPGQTDELPPIPGKRYFTIGEVSQLCEVKPHVLRYWEQEFPQ
                     LKPVKRRGNRRYYQRQDVMTIRQIRSLLYDQGFTIGGARQQLAGADAKRDHSQFKLLV
                     RQMIVELEDLLRVLKS"
     gene            complement(2254..>2434)
                     /locus_tag="AC16PP_04960"
     CDS             complement(2254..>2434)
                     /locus_tag="AC16PP_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007943237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6481009.1"
                     /translation="ESPLGGVVSEILLSVPATSIAGGTDEVQRNIISERVLGMPKEPR
                     FDTGPFRDVPKNVLT"
BASE COUNT          436 a          737 c          838 g          423 t
ORIGIN      
        1 cgtggatttc gagctgcagc atgcggccat ggagagagcg acccggctgc tcctggacat
       61 cgttggcggg gagccgggac cggtggtgga ggcggtggaa ccgaaagccc ttccggaggt
      121 gtccgacatc gggttgcgcg agcgtcggct caaccgtctt ctcgggacgg agatcgcgcc
      181 ggagatcgtc gacgaggcca tggagaggct cgacttcgcg gtgacgtcta gggagacggg
      241 agaggagggc gttcgctgga ctgtgcgcgt gcccagccac cggttcgaca tcgtgcgcga
      301 agcggatctc gtcgaagagg tctgccgcat ctacggttac aacaacgtgc cggtgcgcca
      361 gccggtgacc gagctggagc tcacgccggt gcccctcgaa aggagcccgg caggacggct
      421 gaagcggctg ctggcaaccc tcggctatca ggaagccatc acctacagct tcgtggaccc
      481 gcgtctgcag gatctgctcg cgcccgccgc cgatgccctg gcgctgagca atcccatgtc
      541 gtcggagcag tcggtcatgc ggctgaacct gctgcccggc ctcgtcgagg cgctgcgttt
      601 caacgtggcc cggcagcagg accgggtgcg gatattcgag ctggggtcgt gctttgcccc
      661 cggcgacccc gtgctgcaga agctgatgct tggcggtgtg ctctggggtc ggcggcacgg
      721 ggagaactgg aaccagtcag gcgcggaagt cgacttctac gatctcaagg gcgacgtcga
      781 ggccatcgtg gggtggagcg gcgagccggt ggcctacgag cggctcgaag acccggtgct
      841 tcaccctggc caggcggcgc tcgtggtggg ttcgcgaggg ccggtggggc ggctggggcg
      901 gctgcatccg gagatcgaac gcgcgctgga tctgccgggg cacgtcttca tcttcgagct
      961 ggaggcagac gcggttctgc atcgcgcccg ccgcaggcat ctgagcctgt cgaagttccc
     1021 cagcgtcagg cgggacctgg ccgtagtggt tcaggccgat acggcctctt cgagcatcga
     1081 gaggatcctc cgcgagtccc tgggcgaaat tctcgtcgac ttcaggatat ttgacgtcta
     1141 tcagggcaaa ggtattgatt ctaatgagaa aagccttggt atagggttga cgttgcagca
     1201 tccgtcggcc acccttaacg accaggaaat tggcaaatac atgaacgacg ccgtggacgc
     1261 cctggttcga ggtgtgggcg caaggctgcg ttgaagaatc ggacgcccgt ccccgtgatc
     1321 tcaggggtcg ggtgccgaaa acacaggaga ctcccgggtg ggtgccctta ccaaagcgca
     1381 gatggccgac cgcctcttcg atgagctcgg gctgaacaag cgcgaagcca aggagatcgt
     1441 cgagatgttt ttcgacgagg tccgggactc gctggagaac aaccagcagg tcaagctctc
     1501 cggattcggg aacttcgatc tgcgtgagaa gggagagcgg ccgggccgca atccgaaaac
     1561 tggggaagag atccccatca cggcccggcg cgtcgtgacg ttccgacccg ggcagaaact
     1621 gaaggcgcga gtggaagctt atgctggacc cgggtcagac tgacgagctg ccgcccattc
     1681 ccggaaagcg gtacttcacc atcggtgagg tgagccagct gtgcgaggtc aagccccacg
     1741 tgctgcgcta ctgggagcag gaattccccc agctcaagcc cgtcaagcgt cgcggcaacc
     1801 gccgttacta ccagcgtcag gacgtcatga ccatccgcca gatccggtcg ctgctctatg
     1861 accaggggtt tacgatcggg ggcgcccgac aacagctggc gggcgcagac gccaaacgag
     1921 accactccca gttcaagctc ctggtccggc agatgatcgt ggagctcgag gacctgctga
     1981 gggtcctcaa gtcctgactc ccggggcgcg gcagcgatct tccatgcatg acgatgatgg
     2041 cacggcaggc gcaggcaggg aggaggggtc cggctccata cgggtcgcca tcgtgatctt
     2101 cctggttacc tttgtgctgc tcatggtcat ggggatcatc tggatgttca ttcgcgtcga
     2161 aggctcgtcc tctggcggcc aagctacggg caccggcgaa aacgcgtccg tctgccgttg
     2221 cctggccgcc gcctccccag cgcgcggcga gcctcaggtc agcacgtttt tcggcacgtc
     2281 gcgaaacggg cccgtgtcaa agcgcggctc tttcggcatg ccgaggacac gctcggagat
     2341 gatgttccgc tgcacctcgt ccgtgccgcc ggcgatggac gtggccggca ccgacaacag
     2401 gatttccgac accacgccgc cgagagggct ctcc
//
LOCUS       DBNHKV010000155         4149 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1226318, whole genome shotgun sequence.
ACCESSION   DBNHKV010000155 DBNHKV010000000
VERSION     DBNHKV010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1226318"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="AC16PP_04965"
     CDS             complement(<1..240)
                     /locus_tag="AC16PP_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HIR6481010.1"
                     /translation="MHPGDFDYELPSGQIAQAPASRRDASKLMHIAGDGIEHLGFHRF
                     PELLMPGDLLVLNDTRVIKARLHGVKDSGGQAEVLV"
     gene            370..714
                     /locus_tag="AC16PP_04970"
     CDS             370..714
                     /locus_tag="AC16PP_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481011.1"
                     /translation="MIIRHMNRSYNRQEAEAEIRDRGLHEMVFEVPAVDNEPHWHDFD
                     AYLYLLDGTLHLRDVQAGVVHECSPGMRIDVPAKTVHAEQSKGYTVLLGCTRDPATFD
                     EGLNRDPATLPD"
     gene            complement(755..1708)
                     /locus_tag="AC16PP_04975"
     CDS             complement(755..1708)
                     /locus_tag="AC16PP_04975"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6481012.1"
                     /translation="MDGSPVRDVSDAEREAYARDGFVRLKNIYPLSWVELLEAQLEDV
                     FEHQPHRAMDQRSVTGGRTQGIRVDMAALANGMIAAVPDASIAVEGGRDAPIVGRSIV
                     ETDAASWHSGIRRHHLEGPLPEIVADLTGTGKIVFYADQLFLKEPGSRIRTPFHQDEP
                     YFLVQGEVAVCWVPVDPVTRENGCMGYVRGSHRWGRLFKPSDFVTDDGTFPERDGIEH
                     SGLETLPPIDVEGPDVVYLEADPGDVIVHHWSTVHGAAGNVSGSATRRAASVRYALGD
                     SRYFRRASSPEPFRDTLDLRDGDPLDRAPRFPVVWPRVRRR"
     gene            complement(1712..2536)
                     /locus_tag="AC16PP_04980"
     CDS             complement(1712..2536)
                     /locus_tag="AC16PP_04980"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HIR6481013.1"
                     /translation="MSGACLQRYCETSLNYGDGDSARTVQVTIRNARTTPDLEFEECG
                     FSRLDHVSEVHDWSDAAHLADVHMAEVRELAQGFLGCDHALAYPPIVRSPASARAVAD
                     YAPIEFVHSDFTDDYLDMIRDPDRAYGAFLAPALETIGVRQDEIAKAERVVMLQFWRN
                     VGARRPDRPLALCDAASVPRSDLFSILVPEYGGQRLEFETFGVRCPKDPDRHRWYTYP
                     DLTAEEVIVFRTYDSRCAAEGRPFWTPHSAFRDPRAGTDPAQRESVEMRVLCFFGL"
     gene            complement(2547..3233)
                     /locus_tag="AC16PP_04985"
     CDS             complement(2547..3233)
                     /locus_tag="AC16PP_04985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR6481014.1"
                     /translation="MQKLYTWFAVIGFGSVAASLMVGFRHDPAAPAVNYLLNAGLFLA
                     YMVVHYVMMTPGFKKLVSSNPAGSPAERRLYMVVAIASWIAVYLIHYPMPGPALALPG
                     WVTFLGGCAFMMSFLAFTEGTTFEGIRAFAGVPGHEQTHGASAEAPLQTEGSYASVRH
                     PMYRGACYMGLTSLLLHPNAAQLAWAGIMGLTFLTFIPIEEKQLLKGRGDEYREYMKQ
                     TPYRIFRGVW"
     gene            3367..>4149
                     /locus_tag="AC16PP_04990"
     CDS             3367..>4149
                     /locus_tag="AC16PP_04990"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6481015.1"
                     /translation="MTIEIVNHTPEPAVIANYSTARYVDRDYMEREWEHVWRRSWLLA
                     GLESDVQKPGDYFVFDMGREQILVTRTASGNVQGFYNVCQHRGNLLVAGERGHADSFR
                     CAYHAWTYDTEGSLKIIPYQERFARGVPCAERSLRKVHTDVWDGFVFVSLAEEPEPLL
                     DFLGPVVEMLAPYRFRNMVLVEDQTVHHKCNWKAVVDNFSELYHVDFLHPQHKRMVDC
                     CNDTVHLFPRGHTGLAVPGATVNPRFPIPDEPTDLQSAQLSSI"
BASE COUNT          805 a         1345 c         1300 g          699 t
ORIGIN      
        1 gaccagcacc tcggcctgcc cgccgctgtc cttgacgcca tgcagccgcg ccttgatgac
       61 acgggtgtcg ttgagcacga gcagatcgcc gggcatcagc agctcgggaa accgatgaaa
      121 gccgaggtgc tcgatcccat cgccggcaat gtgcatgagc ttgctggcgt ctctcctgga
      181 cgcgggagcc tgggcaatct gcccgcttgg gagctcatag tcgaagtcgc ctgggtgcat
      241 tggttttacc gaccaacgga actgcgcagg ccgcggattc taatccggcg cgtgcaggag
      301 caaaagcgcg ccctgctggg gcataatggc agcgctttca gccacgctca cggaggcccg
      361 ggaaatcaga tgatcatcag gcacatgaac cgctcgtaca accggcagga agccgaggcg
      421 gaaattcgcg accggggtct ccacgagatg gtcttcgagg tccccgccgt cgacaacgag
      481 ccgcactggc acgatttcga cgcctatctc tacctgctcg atggcacgct gcacctgcgg
      541 gacgtgcaag ccggcgtggt gcacgagtgc tccccgggca tgcgaatcga cgtgccggcc
      601 aagaccgtgc acgcagagca aagcaaaggc tacacggtgc tgctcggctg tacccgggac
      661 ccggcgacct ttgacgaggg gctcaatcgg gatcctgcta ccctgcccga ctgatcggcc
      721 ggacgaagac ggacccgact agctgacgag ccggctaccg ccgccgcacc cgcggccaga
      781 ccaccggaaa ccttggcgcg cgatccagag ggtcgccgtc acgaaggtcg agagtatccc
      841 gaaacggctc cggggatgat gctctgcgga agtagcggga gtcgcctagc gcgtagcgca
      901 cggaggcggc ccgccgggtg gccgagccgg agacgttccc cgcagcgcca tgaacggtcg
      961 accagtgatg cacgatcacg tcgcccggat ccgcctccag atagaccacg tcagggcctt
     1021 cgacgtcgat gggcggcagg gtctcgagac cggagtgctc aatgccgtcc ctctcgggga
     1081 atgtcccgtc gtccgtgacg aagtcgctgg gcttgaacag gcgtccccag cggtgcgaac
     1141 cgcgcacgta gcccatgcaa ccgttctccc gggtcacggg atccacgggc acccagcaca
     1201 cggcaacctc gccctgcacg agaaagtaag gctcgtcctg gtgaaagggt gtacggatgc
     1261 gcgagccggg ctccttgaga aacagctggt ccgcgtaaaa gacaattttg cccgtgcctg
     1321 tgaggtcggc aacgatttcc ggcagcggcc cttcgagatg atggcgccgg attccagaat
     1381 gccagctggc ggcatccgtc tccacgatgc tgcgtccgac gatgggcgcg tctcttcctc
     1441 cctcgacggc aatcgacgca tccgggaccg ccgctatcat tccgttggca agcgccgcca
     1501 tgtccacccg aatgccttgc gtgcgtccgc ccgtcaccga acgctggtcc atggccctgt
     1561 gaggttgatg ttcgaaaacg tcttcgagct gggcttcgag caactcgacc caggacaagg
     1621 gatagatgtt cttcagccgg acgaaaccgt cccgtgcata ggcctcgcgc tcggcgtccg
     1681 acacgtctct gaccggcgag ccgtccacgg cttagaggcc gaagaagcag agcacgcgca
     1741 tctccacgct ttcgcgctga gcgggatccg tacccgcgcg cggatctctg aatgcggaat
     1801 gaggagtcca gaagggccgg ccctcggccg cgcagcggct gtcgtaagtg cgaaacacga
     1861 tgacctcttc ggcagtcagg tccgggtacg tgtaccagcg atgccggtcc gggtccttcg
     1921 gacagcgcac tccgaaggtt tcgaactcca gccgctggcc gccgtactcc ggcaccagga
     1981 tggaaaacag atccgagcgg ggcacgctgg ccgcgtcgca cagggcgagc ggtcggtccg
     2041 gtcgtcgggc ccccacgttg cgccagaact gcagcatgac cacgcgttcc gctttcgcga
     2101 tctcatcctg acggacaccg atggtctcca gagccggcgc gagaaaggcg ccgtaagctc
     2161 gatccgggtc ccggatcatg tcgagatagt cgtcggtgaa gtccgaatga acgaactcga
     2221 tgggcgcgta gtcggccact gcgcgggcac tggcagggct gcggacgatc ggcggatacg
     2281 ccagcgcgtg atcgcaaccg aggaacccct gcgccagttc tcgcacttcg gccatgtgaa
     2341 cgtcggcaag gtgggcagcg tcgctccagt cgtggacctc cgagacatga tccagtcggg
     2401 aaaaaccgca ctcctcgaat tcgagatccg gcgtcgttct tgcattgcgg atcgtaacct
     2461 ggaccgttcg ggccgaatcg ccgtcaccgt agttcagcga tgtttcgcag tagcgctgca
     2521 gacaagcgcc ggacatcgcg cggcgcctac cagacgcctc ggaatatgcg gtaaggcgtc
     2581 tgcttcatgt actccctgta ctcatcgcca cgtcctttga gcaactgctt ctcctcgatg
     2641 ggtatgaacg tcaggaacgt gagccccata atccccgccc aggccagctg cgccgcgttg
     2701 ggatgaagca gcagggaggt cagccccatg taacacgcgc cccggtacat tggatgtctg
     2761 acgctggcgt aggaaccctc cgtctgcagc ggcgcttcgg cagaagcgcc atgcgtctgc
     2821 tcgtgtcccg gtactcccgc aaaggcccga atcccttcga acgtggtccc ttctgtgaac
     2881 gcgagaaaag acatcatgaa cgcgcaacct cctagaaacg tcacccagcc cggaagcgcc
     2941 agcgcggggc ccggcatcgg atagtgaatc agatacacgg cgatccagga ggcgatggcc
     3001 accaccatgt agagcctgcg ctccgcgggg cttcccgccg gattgctcga aaccagcttc
     3061 ttgaacccgg gcgtcatcat cacgtagtgg acgaccatgt aggccaagaa caagccggcg
     3121 ttgagaaggt agttgacggc cggagctgcg ggatcatggc gaaaccccac catgagagag
     3181 gcggcgaccg aaccgaagcc aatgaccgca aaccaggtat acagcttctg catgctctca
     3241 tcccctcaga aaagagtttc ccgaatatag ccgaaggcgc cgcgcagcgc ctccccgcaa
     3301 gggaggcgtt tccagccgcg accgcggctg gcatcattgg gcgcaggaga agccagcgga
     3361 tccgtcatga ccatcgagat cgtcaaccac acccccgaac ctgccgtcat cgccaactac
     3421 tcgacggccc gctacgtgga ccgcgactac atggagcggg agtgggagca cgtctggcgc
     3481 aggtcctggc tgcttgccgg gttggagagc gacgtgcaga agcccggcga ctatttcgtc
     3541 ttcgacatgg ggcgcgagca gattctggtg acccgaacgg cttccggcaa cgtgcagggc
     3601 ttctacaacg tctgtcagca tcgaggcaac ctgctggtgg ccggcgagcg cggtcacgcc
     3661 gacagcttcc gctgcgccta ccacgcgtgg acgtacgata ccgaaggctc tctgaagatc
     3721 attccctacc aggagcggtt cgccagaggt gtcccgtgcg ccgagcgatc gctgcggaag
     3781 gtgcacacgg acgtatggga cggtttcgtg ttcgtctccc tcgccgagga gcctgagccg
     3841 ctgctcgatt ttctgggtcc ggtggtggag atgctcgcgc cttaccgctt tcgcaacatg
     3901 gttctggttg aggaccagac ggtacaccac aagtgcaact ggaaggccgt cgtcgacaat
     3961 ttcagcgagc tttaccacgt ggactttctg catccccagc acaaacgaat ggtggactgc
     4021 tgcaacgaca cggtgcatct gtttccccgc gggcacacgg gcctcgccgt gccgggcgcg
     4081 acggtgaacc cgcgattccc gatccccgac gagccgacgg accttcagtc cgcgcagcta
     4141 tcgagcatc
//
LOCUS       DBNHKV010000156         2438 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_21089, whole genome shotgun sequence.
ACCESSION   DBNHKV010000156 DBNHKV010000000
VERSION     DBNHKV010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_21089"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="AC16PP_04995"
     CDS             <1..508
                     /locus_tag="AC16PP_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6481016.1"
                     /translation="MIEMAAEVGDGVIFNLWPKSVLPKMMEHVRIGAERAGKNWEDVE
                     VVNRAMVLCTNDKQAGRNLFRAAFAPYYATPVYNAFLAWAGHADAAGAITEGWAAKDR
                     AMTTGALSDELIDEIAIIGDEDEIRARIQADADGGVHTHIVAPLAASPEDVERTFAAF
                     TADGFSLK"
     gene            550..1392
                     /locus_tag="AC16PP_05000"
     CDS             550..1392
                     /locus_tag="AC16PP_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6481017.1"
                     /translation="MEYQKIIVDEPRPMVRRITLNRPEKRNPLSNELRSEMFHALESA
                     DQDDGVRVTIIRGAGPCFSAGYDLKSDTSQNPPFYTAGGLGHWPRHVVEGFFRMWDMA
                     KPIIAQVHGYCLAGGTELATACDLVYVAEDAKIGYPVVRTMSPPDNQFYPWIVGLRRA
                     MELMLTGDHMSGVEAAESGFANRAFPAEELEERVLAIAERVALTPTDLQQFNKRAVHR
                     QMDAMGIRAGIRAGTEMQQLAFFTDTTRAYFKEMSKGLTHALSKRDEAFGDYRTSASG
                     KAED"
     gene            1426..2103
                     /locus_tag="AC16PP_05005"
     CDS             1426..2103
                     /locus_tag="AC16PP_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6481018.1"
                     /translation="MKRLYLIRHGQTEWNVAKRMQGRLDSPLTSAGRIQAAVHGRLLS
                     EIASLEQLVVSPSGRTRETACILNSHVGAPMRHDEALMERDCGEWSGLTIEEIEQHFP
                     EAWEARREDPFYHRPPGGENHEDLVQRAGPLLAEFLAGDVTEVGLVTHGVMSRAIVTH
                     LLELTPVEAVGIRHPNDLVYRLDICATGVQVSHFLAGDGPRAGLLRQNENETISGPAR
                     AGERRDR"
     gene            2122..>2438
                     /locus_tag="AC16PP_05010"
     CDS             2122..>2438
                     /locus_tag="AC16PP_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6481019.1"
                     /translation="MKVDGGVGWELDLVGAQAQELEEMGYSGILSAETSHDPFFPLLV
                     AAQNTERVDLMTSIAVAFARSPMTLANIGHDLNAVSKGRFVLGLGSQIRAHIMKRFSM
                     PWS"
BASE COUNT          461 a          748 c          815 g          414 t
ORIGIN      
        1 gatgatcgag atggcggccg aggtcgggga cggtgtgatc ttcaatctgt ggcctaagag
       61 cgtgctgccg aagatgatgg agcacgttcg catcggcgcc gagcgcgccg gaaagaactg
      121 ggaagacgta gaggtcgtca accgtgcaat ggttctgtgc acgaacgaca agcaggcggg
      181 ccggaacctg ttccgggctg cctttgcgcc ctattacgcc acccccgttt acaacgcgtt
      241 tcttgcctgg gcgggtcatg ccgatgccgc cggcgccatt accgagggct gggcggcgaa
      301 agaccgggcg atgacgaccg gcgccctgag cgatgagctc atcgacgaga tcgccatcat
      361 cggcgacgag gatgagatcc gcgcgcgcat acaggcggat gcggacggcg gcgtccacac
      421 ccacatcgtc gctcccctgg ccgcctcgcc cgaagacgtg gagcgaacgt tcgccgcgtt
      481 cacggcagac gggttcagtt tgaaataggc accctccggg tccgtcgatc cgaagacagt
      541 gaggagatca tggaatacca gaagatcatc gtcgacgagc cgcggcccat ggtccgccgc
      601 ataacgctca accggcccga gaagcgcaac ccgctctcca acgagctgcg ctcggagatg
      661 ttccacgcgc tggaaagcgc cgatcaggac gatggggttc gggtcaccat catacgcggg
      721 gccggtccgt gcttctcggc gggctacgat ctgaagagcg ataccagcca gaacccgccg
      781 ttctacacgg ccggcggcct tggtcactgg ccgcggcacg tggtggaagg attcttccgc
      841 atgtgggaca tggccaagcc gatcattgcc caggtgcacg gatactgcct ggccggcggt
      901 accgagcttg ccacggcctg cgacctggtt tacgtggcag aggatgccaa gatcggctat
      961 ccggtcgtgc gcaccatgtc gcccccggac aatcagttct acccctggat cgtcggcctc
     1021 aggcgggcca tggagctcat gctcaccggt gatcacatgt cgggggtcga agcggcagaa
     1081 agcggctttg ccaaccgtgc ctttcccgcc gaggagctgg aggagcgggt tctggcgata
     1141 gccgagcgcg tcgccctcac gcccacggat ctgcagcaat tcaacaaacg cgccgtgcat
     1201 cggcagatgg acgccatggg aatccgggcc ggcattcgtg ccggcacgga gatgcagcag
     1261 ctcgccttct tcacggacac cacgcgtgcg tacttcaaag agatgagcaa ggggctgacc
     1321 cacgcgttgt cgaaacgcga tgaggcgttc ggagattacc ggacctccgc gtccggcaag
     1381 gccgaggact gacgggctcc ggtgccggga tctccaacgg gaatcatgaa gcggctctat
     1441 ctgatccgtc acggccagac tgagtggaat gtggcaaagc gcatgcaggg acgcctcgat
     1501 tcgcccctga cctcggccgg tcggatccag gcggccgttc acggtcgtct gctgagcgag
     1561 atcgccagtc tggagcagct ggtggtgtcc ccgtccggac ggacgcgcga aaccgcctgc
     1621 atactgaact cccacgttgg ggcgccgatg cgtcatgacg aagccctcat ggaacgcgac
     1681 tgcggtgagt ggtcggggct caccatcgaa gagatcgagc aacactttcc ggaggcctgg
     1741 gaggcccggc gggaggatcc cttttatcac cggccacctg gcggcgagaa tcacgaggat
     1801 ctggtgcagc gggcgggccc gttgctggcg gagtttctgg ccggagacgt taccgaggtg
     1861 ggcctggtga cccacggcgt catgtcccgg gcgatcgtga cccatctgct cgagctgacg
     1921 cccgtggaag cggtcgggat ccgtcacccc aacgatctgg tctatcggct ggacatctgc
     1981 gcaaccggtg tccaggtcag ccactttctc gcgggagacg gtccgcgggc gggattgctt
     2041 cgccaaaacg agaatgaaac aatctcgggc cccgcgcggg ccggagagcg gcgggaccga
     2101 taaaaaaact gggggaagac gatgaaagtc gacggcggtg tgggttggga actcgatctg
     2161 gtgggcgctc aggcccagga gctcgaggag atgggctatt cgggaatcct gtcggctgaa
     2221 accagccacg acccgttctt cccgctgctc gtggcggcgc agaacacgga gcgggtcgac
     2281 ctgatgacct cgattgcggt ggcttttgcc cgatcgccca tgactctggc caacatcggc
     2341 cacgatctga acgccgtttc caaaggccgt ttcgtgctgg gcctcggctc gcagatccgg
     2401 gcgcacatca tgaagcgatt cagcatgccc tggtctca
//
LOCUS       DBNHKV010000157        11714 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1084770, whole genome shotgun sequence.
ACCESSION   DBNHKV010000157 DBNHKV010000000
VERSION     DBNHKV010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11714)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11714)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11714
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1084770"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="AC16PP_05015"
     CDS             <1..499
                     /locus_tag="AC16PP_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR6481020.1"
                     /translation="ARGKTGRVNGHLVALLTLMKGQPLAYNRDNQEDKEPLFDTVDTL
                     RDCLTALNGLVPAITPNRERMRSAAAAGFTTATDLADYLVRRDVPFRDAHEVVGRAVA
                     HAIGQGKGLDELTIEELRAFSDSIGEDVFSVLTLEGSVSARNHPGGTAPNSVREAAAR
                     ARDRL"
     gene            683..1957
                     /gene="lysA"
                     /locus_tag="AC16PP_05020"
     CDS             683..1957
                     /gene="lysA"
                     /locus_tag="AC16PP_05020"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR6481021.1"
                     /translation="MDAFSITDGVLHAENVPLPRIAEAVGTPTYVYSTAHLKAQYQRL
                     RRALGELDADVCYAVKANANLAILRLFAEQGAGFDIVSGGELQRVISAGGDPARVIFS
                     GVGKSVAEIDFALKLGIRCFNVESASELDCLSERARVLKRQAPMSIRVNPDVDAGTHP
                     YISTGLRSNKFGIPPGQAVELYRRAQADEYLSVHGIDCHIGSQIGTIDPLIDALRNLL
                     ELTDRLSAEGIRLEHVDVGGGLGIRYGDEEELDVEAYGAALCELLRGRELKLLLEPGR
                     YLVGNGGVLLTRTRFLKPTTAETEKNFAVVDAAMNDLIRPALYQAWHEVRPVTPRAGA
                     AELCWDVVGPVCETGDFLAERRELALQEGDLLAVMSAGAYGMVQSSNYNTRPRPAEVL
                     VAGDRFRVVRRRETTRDQLALELEAEHAETWR"
     gene            1954..2805
                     /gene="dapF"
                     /locus_tag="AC16PP_05025"
     CDS             1954..2805
                     /gene="dapF"
                     /locus_tag="AC16PP_05025"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016703183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR6481022.1"
                     /translation="MKLEFAKMHGLGNDFMVLDLISQDFEPNREQIHAWADRRTGIGF
                     DQMLTISAPTDPDADFRYRIYNADGTEVEQCGNGARCAARFIAERKLSPKDTLVLETL
                     AGPISTALLDAETVEVDLGVPSTRAEDVPFSPEAACGAQGDAVGTFTLRAGEGEHEVT
                     PVSVGNPHGVLFVTQITDAPVDAVGASLTRHPFFPEGANIGFCQVVDPHFIRLRVYER
                     GVGETRACGTGACAAVVAGRLLGHLEDRVKVSLPGGKVRIAWQGPETTVRMAGPATLV
                     YEGHMSL"
     gene            2802..3476
                     /locus_tag="AC16PP_05030"
     CDS             2802..3476
                     /locus_tag="AC16PP_05030"
                     /inference="COORDINATES: protein motif:HMM:NF016243.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HIR6481023.1"
                     /translation="MTAAQDEPTRLTDKDVISYLRLDPDFFQRHSKLLSELNLPHDTG
                     GAVSLIERQVAILRERNMAMRRRMNELVQAAKANETLFEKTRSLTLELLHVDTWHALN
                     EVLATYVLVDFHADFVCCHLRNSEIRMDHVRGHGEELPNERYSRSIDPACVTLRADEL
                     EVLFPAENHDEGGSAVLAPLVLNEAIGCLAIGSRDPAHFESDMDTLFVAYIAEVVARV
                     VQRLSP"
     gene            3473..4351
                     /gene="xerC"
                     /locus_tag="AC16PP_05035"
     CDS             3473..4351
                     /gene="xerC"
                     /locus_tag="AC16PP_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HIR6481024.1"
                     /translation="MTDPAVKRFQDYLRVEKRYSPNTLAAYRRDLERLGAVARVPWTE
                     IRGHHVSTFVAKLHAEGLAPRSIQRALSSIRSFYSYLEKIKLVASNPAAGIKAPKTRM
                     KLPKTLDTDQASQLFAFEAKTPIQKRDRAMAELLYGSGIRLSELVGIDVGDMDLSAGF
                     VRVVGKGNKVRQVPMGGHCIAAIEDWLAARGHPAGDAPLFTGRGERRISPRTVQLRLK
                     RLATQQLGSNQLHPHMLRHSFASHMLESSSDLRAVQELLGHADISTTQIYTHLDFQHL
                     AKVYDAAHPRAQSQDD"
     gene            4366..5082
                     /locus_tag="AC16PP_05040"
     CDS             4366..5082
                     /locus_tag="AC16PP_05040"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6481025.1"
                     /translation="MPELKVITFDLDNTLWDVDRVIINAEQRMRTWLLAHVPAFHERF
                     PPETIFELRTEIVNRQPHLKHDLSRLREEVLYQAISRCGYPEALAREYAHQAFQLFFE
                     ARHEVEFFEGALESLAILAKHYVLGALTNGNADFTKLKLDGYFSFGYSAASVGAGKPA
                     PDMFHAALEHTGVKPEQVVHVGDHPVDDIQGASQLGMHTIWVNLSGEALPPGTPKPSD
                     EVARVHDIPTAVSRIAGRGR"
     gene            complement(5095..6456)
                     /gene="thrC"
                     /locus_tag="AC16PP_05045"
     CDS             complement(5095..6456)
                     /gene="thrC"
                     /locus_tag="AC16PP_05045"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR6481026.1"
                     /translation="MRYVSTRGETASLGFTDAVLTGLAPDGGLLVPEHVPQVSARLDA
                     WADLSFTDLACEIIPLFVDDMSRQDLNVLIRQAYACFDHDEVVPFVRLGDLEVLELFH
                     GPTLAFKDIALQLLGRLFEHILSARGRHLNILGATSGDTGSAAIAGVRGLPDIDIFVM
                     YPHGRISPLQELQMTTVLDDNVHCLSVDGSFDDCQTLMKTVFADLAFKERYALGAVNS
                     VNWARVLAQIVYYGYASLKRGMEFPVSFCVPTGNFGNVFAGYLAKRMGFPIERLILAT
                     NENDILAVFFETGTYQRGDVRFTVTPAMDIQVASNFERFLYYHMGEDSRPLRAFMTEF
                     AETGRAVAAIPGDDDFVATAVSVAETVEAIRATYSEFGYVADPHTAVGIAAAGRFPAE
                     GAKICLATAHPAKFPETVNGAVGAEVAVHPALEALSGLESRTTRIPATVEAVKEAIQA
                     SAR"
     gene            complement(6561..7802)
                     /locus_tag="AC16PP_05050"
     CDS             complement(6561..7802)
                     /locus_tag="AC16PP_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR6481027.1"
                     /translation="MTQTNYALDTFYFLVMGALVMFMAPGFAMLEAGLVRSKNTAEIL
                     LKNVALFGIACIMYLICGYYIMYVGAVEGGILPDFGLWIGAENTVDETLASGGDVYYS
                     ARSDYFFQVVFVATAMSIVSGAVAERMKLWAFLAFAVVMTGLIYPLQGMWTWGGGWIS
                     TAGYSDFAGSGIVHMCGAAAALAGVLLLGPRKGKYNKDGSVNALPGANLPLATLGMFI
                     LWFGWFGFNGGSELKTSNIDEANAVAQVFVNTNIAACGGLVAALLTAKMLFGKADLTM
                     ALNGALAGLVAITADPLSPIAQWAMIIGAVGGVLVVFSIVTLDKLKIDDPVGAISVHG
                     VVGIWGILAVVFNNGDATIGGQLIGIAGIFAWTFVAALVVWGILKATMGIRVSEEEEY
                     EGVDLSECGLEAYPEFAKVSS"
     gene            complement(7856..8194)
                     /gene="glnK"
                     /locus_tag="AC16PP_05055"
     CDS             complement(7856..8194)
                     /gene="glnK"
                     /locus_tag="AC16PP_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR6481028.1"
                     /translation="MKLVTAIIKPFKLDDVREALSDIGVQGMTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEIAIDDGLLEQCLETITKSANTGKVGDGKIFVSSLEDVVRI
                     RTGETGAEAV"
     gene            complement(8323..9072)
                     /locus_tag="AC16PP_05060"
     CDS             complement(8323..9072)
                     /locus_tag="AC16PP_05060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HIR6481029.1"
                     /translation="MKKIITPVVAGILLFAAGASAPAAAEGPEFSGNIAYLTDYRFRG
                     ISQGDRSQALQGGFDIELDNGLYAGTWASNVGFTECCGSAGLELNYYAGFAGAFSEEV
                     DYDVGFLWYNYPEDDADPDLDYYELYGSVSAFGGTFGAAFSPDYFAETGDFWYLYGDY
                     SFGVAENLSIDLHVGFNLFEDEESFAAFIGPEDGDAGDNYIDYSIGVSTSALGLDFGL
                     SFIGTDLKKSECFGGTKLCDDTVVLSVSKSL"
     gene            9446..9721
                     /locus_tag="AC16PP_05065"
     CDS             9446..9721
                     /locus_tag="AC16PP_05065"
                     /inference="COORDINATES: protein motif:HMM:NF016280.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HIR6481030.1"
                     /translation="MAEHSARNHSPGNEDVVGTFIERLTEQLASLVPPDSLRLVLDQA
                     RSALQRGLAEVDLVPRHELDAHLAALDRLTETTRDLERRVAALEADS"
     gene            9732..11282
                     /locus_tag="AC16PP_05070"
     CDS             9732..11282
                     /locus_tag="AC16PP_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR6481031.1"
                     /translation="MNDPDGHTLKSATEKSLTRTRAQAGMDTPAVTVETHLPGGLPAF
                     TLVGLPETAVRESRDRVKSAIDNCGLSFPNGKVVVNLAPADLAKEGARLDLAIAVSIL
                     AATGQLPHRVLEGFEFLGELGLSGELRPIRGCLCAALASATEVHERRLVLPSANAGEL
                     AMAPPGRVVPLAHLREVVRLLREPGYQPAAAPSAKPAANAERESRSLAEVIGQPGAKR
                     ALAVAAAGGHHLLMTGPPGTGKTMLARRLAGLLPALTEAEAREAALVYSAAGLPLEYP
                     FVPPFRDPHHTASGPALVGGGGHPQPGEISLAHRGVLFLDELPLFKPSVLEVLREPLE
                     TRSITIARARYRARFPASFQLVAAMNPCPAGRVCSESACRCTPEQVRRYRARISGPLL
                     DRIDLHVAVPPIPKALLLAGARDSSSGVDQTKAAIAGARRVQLSRQGRLNVDLTGADA
                     TRLTRLNTESRRLLANAVDRFQLSARGFHRVLKVARTVADIEGHVDVGPPHVAEALSY
                     RSVTWEGE"
     gene            complement(11287..>11714)
                     /locus_tag="AC16PP_05075"
     CDS             complement(11287..>11714)
                     /locus_tag="AC16PP_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6481032.1"
                     /translation="GVALVAHAVPPGSEDEIRARTAPMGALCRAGENCSGFGAPAAMS
                     APAVAGAQAPAGAPAAAAQSGEQIYNQFCVACHAVGVANAPKLGDVAAWADRIAKGMD
                     VLMASTLNGINAMPAKGTCMTCTDADLQAAVEYMVDGSR"
BASE COUNT         2124 a         3884 c         3776 g         1930 t
ORIGIN      
        1 ggcgcgcggc aagacggggc gggtgaacgg gcatctggtc gcgctgctca cgctcatgaa
       61 aggtcagccg ctcgcgtaca accgcgacaa tcaagaggac aaggagccgc tgttcgacac
      121 ggtggacacc ctgcgcgatt gcctgacggc tctgaacggc ctcgtcccgg ccatcacccc
      181 caatcgggag cggatgcgtt cggccgccgc cgcgggcttt accaccgcga cggacctggc
      241 cgattacctg gtgcggcgcg atgtgccctt ccgggacgcc cacgaggtgg tgggccgcgc
      301 cgtggcgcac gcgatcgggc agggcaaggg tctcgacgag ctgacgatcg aggagctgcg
      361 cgcgttcagc gacagcatag gcgaggacgt gttttccgtg ctcacgctcg aggggtccgt
      421 cagtgcgcgc aatcacccgg gcggcaccgc ccccaacagc gtgcgcgagg cggcagcccg
      481 cgcccgcgac cggctttgac cgcggccgct gcgccgccat gaggcggtgt atactgactc
      541 gttcggccta gctgcgagcc accggtgcgc tggatcctgt taatcattct tgcctctcag
      601 gcggtcatgt gcggtcagaa gggtcccctg gagctgcccg acgctcagtc agaagcgccc
      661 gccgccagcc accggccctg acgtggacgc cttctccatc acagacgggg tgctccacgc
      721 ggagaacgtg ccgctgccgc ggattgccga ggcggtcggc acgccgacgt acgtctactc
      781 gaccgcccac ctgaaggcgc agtaccagcg gctgcgccgc gcgctcggcg agctcgacgc
      841 ggacgtctgc tacgcggtca aggccaacgc caatctggcc attctgaggc tcttcgctga
      901 gcagggcgcc ggtttcgaca tcgtctccgg cggcgagctg cagcgggtca tcagcgcagg
      961 aggcgaccct gccagggtga ttttctccgg ggtaggcaag tcggtggccg agatcgactt
     1021 cgcgctcaag ctcggcatcc gctgcttcaa cgtggagagc gccagcgagc tcgactgcct
     1081 gagcgagcgg gcacgggtgc tcaagcgcca ggcgcccatg tccatccggg tcaacccgga
     1141 cgtggatgcc gggacgcatc cctacatctc cacggggtta cgcagcaaca agttcggtat
     1201 accgccgggc caggcggtcg agctgtacag gcgggcgcag gcagacgagt acctatccgt
     1261 gcacgggatc gactgccaca tcggctccca gatcggcacc atcgacccgc tgatcgacgc
     1321 ccttcgcaac ctgctggaac tcaccgaccg gctgtccgcg gagggaatcc gcctggaaca
     1381 cgtggacgtg ggcggcgggc tgggcatccg ctacggggac gaggaggagc tcgacgtcga
     1441 ggcatacggt gcggcccttt gcgagctgct gcgcggccgg gaactcaagc tcctgctgga
     1501 acccggcagg tatctggtgg ggaacggcgg cgtgctgctg acccgaacgc gattcctgaa
     1561 accaacgacg gccgagaccg agaagaactt cgcggtggtc gacgctgcca tgaacgatct
     1621 gatccgcccg gcgctgtacc aggcgtggca cgaggtccgt cccgtcacgc cgcgagcggg
     1681 ggcggccgag ctgtgctggg acgtggtggg ccccgtctgc gagacgggcg acttcctggc
     1741 cgaacgcaga gaactggccc ttcaggaagg cgaccttctg gccgtcatgt ccgccggggc
     1801 ctacgggatg gtgcagagct ccaactacaa cacgcgtccc agacccgcgg aggttctggt
     1861 cgccggagac cgcttccggg tggtgcggcg ccgcgaaacc actcgcgatc agctcgccct
     1921 ggagctcgag gcggagcacg cggagacgtg gcgatgaagc tcgagttcgc gaagatgcac
     1981 gggctcggta acgacttcat ggtgctggac ctgatcagcc aggatttcga gccgaaccgg
     2041 gaacagattc acgcctgggc cgatcgccgc accgggatcg gcttcgatca gatgctcacc
     2101 atctcggcgc ccaccgaccc ggatgcggac ttccgctacc gcatctacaa cgcggacggc
     2161 accgaggtgg agcagtgcgg caacggcgcc cgatgcgcgg ccagattcat cgccgagcga
     2221 aagctgagcc cgaaagacac cctggtgctc gagacgttgg cggggcccat atccacggcc
     2281 ctgctggacg ccgagaccgt ggaggtcgat ctgggggttc caagcacccg ggccgaagac
     2341 gtgccgttca gcccggaagc ggcgtgcggc gcgcagggcg atgcggtcgg gaccttcacg
     2401 ttgcgcgcgg gggagggcga gcatgaggtg accccggtct ccgtcgggaa tcctcacggg
     2461 gtgctcttcg taacgcagat caccgatgcg cccgtcgacg cggtgggggc cagcctgacc
     2521 cgccacccgt tctttcccga gggcgcgaac atcgggttct gccaggtggt ggatccgcat
     2581 ttcatccggc tgcgcgtcta cgagcgaggg gtgggcgaaa cgcgggcttg cggaacgggc
     2641 gcatgcgccg ccgttgtcgc cggccggctg ctgggccacc tggaagaccg ggtgaaggta
     2701 tcgttgccgg gaggcaaggt ccgcatcgcc tggcagggcc cggagaccac ggttagaatg
     2761 gccggacccg ccacactggt ttacgaaggc cacatgagtc tatgaccgcg gcccaggacg
     2821 agccgacgag gcttaccgac aaagacgtca tttcttacct gcgactcgac cccgacttct
     2881 tccagcggca ctcgaagctg ctttccgagc tcaacctgcc ccacgacacg ggcggcgcgg
     2941 tctcgctgat cgagcgtcag gtagccatcc ttcgcgaacg caacatggcg atgcggcgcc
     3001 ggatgaacga gctcgttcag gcggccaagg ccaacgagac gttgttcgag aagacgcgtt
     3061 ccctgacgct ggagctgctc cacgtggaca cgtggcacgc tctgaacgag gtgctcgcga
     3121 cctacgtgct ggtggatttc catgcggact tcgtatgctg ccatctgagg aattcggaga
     3181 tccgcatgga ccacgtgcgg ggccacggcg aagaacttcc caacgagcgc tactccagaa
     3241 gcatcgatcc cgcgtgcgtc accctgcgcg ccgacgagct cgaggtgctt ttccccgcgg
     3301 agaatcacga tgagggcggc agcgcggtgc tggcaccgct ggtgctcaac gaggcgatcg
     3361 gctgtctcgc catcggcagc cgcgatcctg ctcacttcga atcagacatg gatacgctgt
     3421 tcgtcgccta catcgcggag gtggtcgcgc gggtggtcca gcgcctgtcg ccgtgaccga
     3481 tcccgccgtc aagcggttcc aggactacct gcgggtcgag aagcgctact cacccaacac
     3541 gctggccgcc taccggcggg atctggaacg gctgggcgcg gtggccaggg tgccctggac
     3601 cgagatccgc ggacaccacg tgagcacgtt cgttgccaag ctgcacgccg aagggctggc
     3661 tccgcgaagc atccagcgcg ccctgtcgag catccgttcc ttctacagct atctggagaa
     3721 gatcaagctg gtcgccagca accccgcagc cggcatcaaa gcgccaaaga cccggatgaa
     3781 gctgcccaag acgctcgata cggatcaggc ctcacagctg ttcgccttcg aggccaaaac
     3841 gccgattcag aaacgtgacc gcgcgatggc cgagctgctc tacggcagcg gaattcgcct
     3901 gtccgagctg gtcggcatcg acgtcggcga catggacctg agcgccgggt tcgtgagggt
     3961 cgtcggcaaa ggcaacaaag tcaggcaggt ccccatgggc gggcactgca tcgcggccat
     4021 cgaagactgg ctggcagccc ggggacatcc cgccggcgac gcgccgctgt tcaccggtcg
     4081 gggcgagcgg cgtatcagcc cccgaaccgt ccagctgcgc ctaaagcgcc tggccaccca
     4141 gcagctcggc agcaaccagc tgcacccgca catgctcagg cacagctttg ccagtcacat
     4201 gctcgagtcg tccagcgacc tgcgggcggt tcaggaactt ctcggccacg ccgacatctc
     4261 aacaacccag atctacacac atctggactt ccagcatctg gcaaaggtgt acgacgccgc
     4321 ccatccccgc gcgcaatccc aggacgactg attaggcccc tgatcgtgcc cgagctgaaa
     4381 gtcatcacct tcgatctcga caacaccctg tgggatgtgg atcgggtcat catcaacgcc
     4441 gagcagcgca tgcggacgtg gctgctcgca cacgttccag ccttccacga acgttttccg
     4501 ccggagacga tcttcgagct gcgcacggaa atagtgaaca ggcagcccca tctgaaacac
     4561 gacctgtccc gactgcgcga agaggttctc taccaggcca tcagccgctg cggctacccg
     4621 gaagcgctgg cccgagaata cgcccatcag gcctttcagc tgttcttcga ggcgcgccac
     4681 gaggtggagt tcttcgaggg tgcgctggaa agcctggcca tcctggccaa gcactacgtc
     4741 ctcggcgctc tgaccaacgg caacgccgac ttcaccaagc tcaagctgga cggctatttt
     4801 tctttcggtt actccgcggc cagcgtcggt gccggcaagc ccgcgccgga catgtttcat
     4861 gcggccctcg agcacacggg agtgaagccc gagcaggtgg tccacgtggg cgaccacccg
     4921 gtcgacgata tccagggcgc atcccagctc ggcatgcaca ccatctgggt caacctgagc
     4981 ggcgaggcgc tgccgcccgg gaccccgaag ccctcggacg aggtcgcaag ggtgcacgac
     5041 attcccacgg ccgtatcccg catcgccggg cgcggccgct gatccgaatc gccctcagcg
     5101 ggcgctggcc tgaatcgcct ccttgaccgc ctcgacggtg gccggaatcc gggtcgtacg
     5161 cgactcgagt cccgacaggg cctccagggc cgggtgcacg gcgacctcgg cacccaccgc
     5221 cccattgacc gtttccggga acttggccgg gtgcgcggtg gcaaggcaga tcttcgcgcc
     5281 ttcggccggg aagcggccgg cagcagcgat gccgacggcc gtgtgcggat cggctacgta
     5341 gccgaactcc gagtaggtgg cgcggatcgc ttcgacggtc tcggccacag agactgcggt
     5401 ggccacgaag tcgtcgtcgc cgggtatcgc ggcaaccgca cgaccggttt cggcgaactc
     5461 ggtcatgaac gccctcagcg gtcggctgtc ctcgcccatg tggtagtaaa ggaaccgctc
     5521 gaaattgctg gccacctgaa tgtccatggc gggcgtcacg gtaaatcgca cgtcgcctcg
     5581 ctggtaggtg cccgtttcga agaacacggc gaggatgtcg ttctcgttgg ttgccagaat
     5641 cagccgttcg atgggaaatc ccatgcgctt ggccaggtag cccgcgaaga cgttgccgaa
     5701 gtttccggtg ggcacgcaga aggagacggg aaactccatg ccccgcttca ggctcgcgta
     5761 gccgtagtag acgatctgcg ccagcactct cgcccagttc acggagttga cggcgcccag
     5821 cgcgtaacgt tccttgaatg ccagatccgc gaagacggtt ttcatgagcg tctggcagtc
     5881 gtcgaagctg ccgtccaccg aaaggcaatg gacgttgtcg tccagcaccg tggtcatctg
     5941 cagctcctgc agcgggctga tacgcccgtg gggatacatg acgaagatgt ctatgtcggg
     6001 cagcccgcga acgccggcga tggccgcgct gccggtatcg ccgctggtcg caccgagaat
     6061 gttcagatgc cggccgcggg ccgagaggat gtgctcgaag agacggccga gaagctgcag
     6121 agcgatgtcc ttgaaggcca gggtaggccc gtgaaagagc tccagcacct ccagatctcc
     6181 cagccggacg aacggaacca cctcgtcgtg gtcgaagcag gcgtatgcct gacggatcag
     6241 gacgttcaga tcttggcgcg acatgtcgtc gacgaaaagc gggatgatct cgcaggcgag
     6301 atcggtgaag gacagatcgg cccacgcatc cagccgggcg ctcacctggg gcacgtgctc
     6361 cggcaccagc aggccgccat ccggggccag accggtcaga acggcatcgg tgaatccgag
     6421 gcttgcagtc tcgccgcggg tgctgacgta tcgcatgggg acgggtgcct gaaaaattgg
     6481 gcattgtacc cgttacagac gaaaacgccg gccacgaatg tggccggcgt tcgttgccgt
     6541 ccgccccgct ggggcgaagc tcaggaggag accttggcga actccggata ggcctccaga
     6601 ccgcactccg aaaggtcgac gccttcgtac tcttcctctt ccgagacccg aatgcccatg
     6661 gtggccttca ggatccccca gacgacgagc gctgcgacga aggtccaggc aaatatcccg
     6721 gcgatgccga tcagctggcc accaatggtg gcgtcgccgt tgttgaagac cacggcgagg
     6781 atgccccaga taccgaccac gccgtggacc gagatggcac ctaccgggtc gtctatcttc
     6841 agcttgtcga gcgtcacgat ggaaaacacc actagaaccc cgccgacggc gccgatgatc
     6901 atcgcccact gagcgatggg cgagagcgga tccgccgtga tggccacgag accggccagc
     6961 gccccgttca gagccatggt cagatcggcc ttcccgaaga gcatcttggc ggtcagcagc
     7021 gcggcaacca agccgccgca ggcagctatg ttggtgttga cgaacacctg ggccaccgcg
     7081 ttcgcctcgt cgatgttcga cgtcttgagc tccgagccgc cgttgaatcc gaaccagccg
     7141 aaccagagga tgaacatccc gagggtcgcc aggggcaggt tggcgccggg cagcgcgttc
     7201 accgacccgt ccttgttgta cttgcccttt cttggcccga gcagcaagac cccggccagc
     7261 gctgccgccg cgccgcacat gtgcacgatg ccggacccgg cgaagtccga gtagccggcc
     7321 gtggagatcc agccgccgcc ccaggtccac atgccctgca gcgggtaaat caggcccgtc
     7381 atgaccaccg caaaggccag gaaggcccat agcttcatgc gctcggcgac tgcccccgag
     7441 acgatggaca ttgcggtagc cacgaaaacc acctggaaga agtagtcgga gcgcgccgag
     7501 tagtagacgt cgcctcccga ggccagggtt tcatccaccg tgttctccgc accgatccac
     7561 aggccgaagt cgggcaggat ccccccctcg acggcgccga cgtacatgat gtagtacccg
     7621 cagatcaggt acatgatgca cgcgatgccg aacagggcga cgttcttcag aaggatttcc
     7681 gccgtgttct tggaccgtac cagtccggcc tcgagcatcg cgaaaccggg cgccatgaac
     7741 atgacgagcg cacccatcac cagaaaataa aaggtgtcca gggcgtaatt agtttgagtc
     7801 aacagatctt ccacaatctt ctccgaattt ttttaaaggc gactagcgca tggcgtcaga
     7861 ccgcctcggc ccccgtctct cccgtgcgga tccgcacgac gtcttcgagc gaggaaacga
     7921 atatcttgcc gtcgcccacc ttgccggtgt tggccgattt ggtgatggtc tccaggcatt
     7981 gctctaagag cccgtcgtcg atggcgatct cgatcttcac cttgggcagg aagtcgacca
     8041 cgtactccgc cccccgatag agttcggtgt gccctttctg gcgaccgaac cctttcactt
     8101 cagtgaccgt catcccctgc actccgatat ccgagagcgc ctcccgtaca tcatcgagtt
     8161 tgaagggctt gatgatggct gttaccagtt tcatgttttg ttctcccgtt gaacccgttt
     8221 tcggtttcta aggtgcacgc tctccccgag cgcagcccgc tgacgcggac tgctctggga
     8281 agatctttgt ggaagatctg tgcaccccgg attctctctc aattacaggc ttttagatac
     8341 cgagagcacg acggtgtcgt cgcacagctt ggtgccgccg aagcactcgc tcttcttgag
     8401 gtccgtgccg atgaaagaga ggccgaagtc gagacccagc gccgaagtgg acacgccgat
     8461 gctgtagtcg atgtagttgt ctccggcatc gccgtcttca ggcccgatga acgctgcgaa
     8521 ggattcctcg tcctcgaaga ggttgaagcc cacgtgcaga tcgatgctca agttctcggc
     8581 gacgccgaag ctgtagtcgc cgtagaggta ccagaagtcg ccggtctcgg cgaagtagtc
     8641 gggagagaag gcagccccga acgtgccgcc gaaggcactg acgctgccgt aaagctcgta
     8701 gtagtcgagg tccgggtccg cgtcgtcctc gggatagttg taccagagga acccgacgtc
     8761 gtaatcgact tcctcgctga acgcaccggc aaaaccagca tagtagttca gctcgagtcc
     8821 cgcggagccg cagcattccg tgaagccgac gttggacgcc caggttcccg cgtagaggcc
     8881 gttgtccagc tcgatgtcga agccgccctg cagggcctgg gaccgatctc cctgggagat
     8941 accgcggaag cggtagtcgg tcaggtatgc gatgttgccg gagaactccg gtccttcggc
     9001 ggcggctggc gcgctggcac ccgctgcgaa gaggaggatt cccgccacaa ccggggtaat
     9061 gatctttttc atgagtaaac ctctgtgctc ttgctctaaa aaccttgtgt cgcgaccggg
     9121 tgatcgctcc ccccttttct gcccaccgca gggcggcaga actgacggga cgcgctcaaa
     9181 ggagcatttt ccgtgccacc agatttttct tcataaaaat cagctggttg aaggaatgct
     9241 tggctggacc ggcaggagat aaccggcttc cgagccgggc tgttgcaccg aaataaggca
     9301 tctgcaccaa agtagcgcgc cagttcagag ccggcctgca ccggcccggg tcggtggctt
     9361 acgcctgggc gaggattccc cagtcgaccg gcgacggcag ctgtgagtac catcgtcgtc
     9421 atccaaacca atgggcggac gcgcaatggc cgagcactca gccagaaacc actcacccgg
     9481 caacgaggac gtggtgggaa cgttcatcga gcgcctcacc gagcagctgg catccctcgt
     9541 gccgcccgac agcctgcgcc tggtcctgga ccaggcccgc tccgcgctgc agcgcggcct
     9601 tgccgaggtg gatctcgttc cccggcacga gctcgacgcc cacctggccg ctctggacag
     9661 gctgaccgaa accacccgtg acttagagcg ccgggtcgct gccctggaag cagattcctg
     9721 atccgatcgg ggtgaacgat ccggacggcc atacgctcaa gtcggccacc gagaaaagcc
     9781 tgacccgtac ccgcgcccag gccggcatgg acacgcctgc ggttacggtg gagacacatc
     9841 ttcccggcgg cctccccgca ttcaccctgg tcgggctccc cgaaaccgcg gtcagagaat
     9901 cccgcgaccg ggtcaagagc gccattgaca actgcgggct gtcgtttccc aacggaaagg
     9961 tggtggtgaa tttagcgccc gccgatctgg ccaaggaagg cgccaggctg gatctggcca
    10021 tcgcggtcag catcctggcg gccaccggcc agctgcccca ccgggttctg gagggattcg
    10081 agtttctggg cgagctgggg ctttccggcg agttgcgccc catccggggc tgcctgtgcg
    10141 ccgcactcgc atccgcaacg gaagtccacg agcggcgcct ggtgctgccg tcggccaatg
    10201 ccggagagct tgccatggcg ccccccggcc gggtggttcc tctcgcccac ctacgcgagg
    10261 tggtgcgtct gctgcgggag ccaggctacc agcccgcggc cgcaccatcc gccaagcccg
    10321 ccgcgaacgc tgaacgggag agccgctccc tggcagaggt gatcgggcag cccggcgcaa
    10381 agcgagcgct ggccgtcgcc gctgccggcg ggcatcacct gctcatgacg gggcctccgg
    10441 gaaccggcaa gacgatgctg gcgcgcaggc tggccggcct gctgcccgcg ctgacggagg
    10501 ccgaagcccg cgaagccgcg ctggtctact cagcggcggg actacccctg gaatatccgt
    10561 tcgtaccccc attcagagac ccgcaccaca cggcttccgg ccctgcgctg gtcggcgggg
    10621 gcggacaccc tcagccgggc gagatctctc tggcacatcg aggcgtgctg ttcttagacg
    10681 agctgcccct gttcaaaccc agcgtgctgg aggtgctgcg cgagccgctc gagacccgct
    10741 cgatcaccat cgcccgagcc cgctaccggg cccgttttcc ggcgagcttt cagctcgtcg
    10801 ccgccatgaa tccctgcccg gcgggtcggg tctgcagcga atccgcctgc cggtgcacgc
    10861 cggagcaggt gcgcagatat cgggcccgca tctccggccc cctgctggac cgcatcgatc
    10921 tccacgtggc agtgcccccg ataccaaagg cgctgctgct ggcgggagcc cgagactcca
    10981 gcagcggcgt tgatcagacg aaggccgcca tcgcaggcgc ccgccgcgtc cagctgtcgc
    11041 gccaggggcg gctcaacgtg gatctcacgg gggccgacgc caccaggctg accagattga
    11101 acacggagag ccgacggctg ctggccaatg cggtggaccg ctttcaacta tccgccaggg
    11161 gattccaccg cgtgctcaaa gtggcgagaa ccgtggcgga catcgagggg catgtagacg
    11221 tggggccacc gcatgttgcg gaagcactga gctaccggtc ggtgacctgg gagggtgagt
    11281 gatcggctac cgggagccgt ctaccatgta ctccacggcc gcctgcagat cggcgtccgt
    11341 gcaggtcatg caggtgccct tggcgggcat ggcgttgatc ccgttgagcg tgctcgccat
    11401 gagcacgtcc atgcctttgg cgatgcggtc cgcccacgcc gccacatctc ccagcttggg
    11461 ggcgttggcc acacccacgg catgacaggc cacacagaac tggttgtaga tctgctcgcc
    11521 ggactgagcc gcagcggccg gcgcccctgc aggggcttga gccccagcga ccgccggggc
    11581 cgacattgcc gccggcgccc caaagcccga gcagttctcg ccggctcggc aaagtgcccc
    11641 catcggcgcg gtgcgggcac ggatctcgtc ctcgctgccg ggcgggaccg catgagcgac
    11701 caaggcgacg ccgc
//
LOCUS       DBNHKV010000158         8645 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_446735, whole genome shotgun sequence.
ACCESSION   DBNHKV010000158 DBNHKV010000000
VERSION     DBNHKV010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8645)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_446735"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="AC16PP_05080"
     CDS             <1..414
                     /locus_tag="AC16PP_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012076207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR6481033.1"
                     /translation="DLAREPIVTYVFGFTGRSRLDDAFAAEGLKPNVVFTATDADVIK
                     TYVRLGLGVGIIATMALDDELDDDLVAIDAAHLFSPSVTHIGFRRGTFLRRYMYDFIE
                     RFAPHLDQQMVDLAAACATSAERAALFEDLELPVR"
     gene            complement(437..1150)
                     /locus_tag="AC16PP_05085"
     CDS             complement(437..1150)
                     /locus_tag="AC16PP_05085"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HIR6481034.1"
                     /translation="MQPSPAPDQRPQEVLADAVGRFAPGHIAISFSGAEDVVLIDMAH
                     QLGLAVDVFSLDTGRLHAETYRFIESVRNRYGVAIEMVMPEPTAVGELVKSKGLFSFY
                     EDGHSECCGVRKIKPLKRKLSTLDAWITGQRQDQSVTRTSVPTEQEDSAFAGRSGSLV
                     KFNPLAQWSSADVWAYIREHGVPYNPLHDRGFVSIGCEPCTRPVGPHEHERSGRWWWE
                     EATRKECGLHAQNVVRIVG"
     gene            1262..1858
                     /gene="thrH"
                     /locus_tag="AC16PP_05090"
     CDS             1262..1858
                     /gene="thrH"
                     /locus_tag="AC16PP_05090"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019689458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HIR6481035.1"
                     /translation="MDVLCLDLEGVLIPEIWQAVADRTGIEELRKTTRDIPVYDDLMG
                     MRLEVLGANDLSMSLIESVIDGLEPLPGASDFLGWARARYQVAIISDTFYQFANPLMA
                     KLGWPTLLCHNLVVENDRIVGYRIRQPDPKRCSVQAFKSLNYRVLAAGDSYNDIAMLE
                     EADAAFFYRAPENVLAEYPQYPCAGSYGELQALLKAAA"
     gene            complement(1928..3232)
                     /gene="aceA"
                     /locus_tag="AC16PP_05095"
     CDS             complement(1928..3232)
                     /gene="aceA"
                     /locus_tag="AC16PP_05095"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019361792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HIR6481036.1"
                     /translation="MALHFIPRKPREEQVRELQTEWDTNPRWKDVKRGYTAADVVNLR
                     GTIPHRSMLAAHGADKLWDYLQNEDFINSLGAVTGGQAVQQVKAGIKAIYCSGWQVAA
                     DANDSETMYPDQSLYAVNSVPTLVNRINNAFRRADEIQWSKGIDDGIDYFAPIIADAE
                     AGFGGILNAFELMKSMIGAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLIAAR
                     LAADVCDVPTILLARTDANAADMVTSDIDPWDKDFLTGERTPEGFYKTHAGLDQAISR
                     GLAYAPYADLLWCETAVPDLDEAKRFAEAIKKEYPDQMLSYNCSPSFNWRANLDDATI
                     AKFQKELAAMGYKYQFITLAGIHSMWFNMFNLAQGYASRQMSAYVELQEEEFAAADRG
                     YTFVSHQQEVGTGYFDQVATVIQGGQSSVTALTGSTEEAQFH"
     gene            complement(3372..4349)
                     /locus_tag="AC16PP_05100"
     CDS             complement(3372..4349)
                     /locus_tag="AC16PP_05100"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF051921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6481037.1"
                     /translation="MDIHVVPVDWSTHQHQLNEVRDAVFVREQGVPQELEWDGLDADA
                     THFLAVNEAGQALGCARLLGTGQIGRMAVVASQRRGGIGRRLLEAAVEKACEDGHAEV
                     FVHAQTEVARFYREAGFVPEGQEFMEADIPHIAMRLILPVEFRPSAEPIPAPLIVPQE
                     SDADDARAELKHLHGEPESIDALAACVAEPTRALLIYSQYLDHSLFDRPEIVDAVSRF
                     VRSAPPATLRILIHSSQPMVSRGHKLLELARRIDSKIEIRKVPDGLATDVHTYLVWDQ
                     RGYWLMPDYREYDGLTNLYDPVQASKLTERFDYLWNLSSPDPELRILRI"
     gene            complement(4349..5719)
                     /gene="purB"
                     /locus_tag="AC16PP_05105"
     CDS             complement(4349..5719)
                     /gene="purB"
                     /locus_tag="AC16PP_05105"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009683124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR6481038.1"
                     /translation="MQTHPLMTIGALDGRYRNRLEPLAPLVSEFGLMRYRVLVECRWF
                     AHLAQTDSIGELPALSSGQAAALAAVAEDFSLADAERIKAIERTTNHDVKAVEYFVKE
                     RVAAIDGLASHIEFVHFACTSEDVNNLSYALMLRDSRELLGEQMQALIDQISAVADDA
                     AEIPMLSRTHGQAASPTTMGKELKNVAYRLSRQLRQLREVALLGKMNGAVGNYNAHVV
                     AYPEVDWPELARAFVEGLGLAQNPLTTQIEPHDYISELFHALVRFNQILLDFDRDMWG
                     YIAIEYFVQQPVAGETGSSTMPHKVNPIDFENSEGNLGIANALLEHMAAKLPVSRWQR
                     DLSDSTVLRNVGSACGHCSVAYQASQRGLGRVKINGDRIRADLDASWEVLAEAVQTVM
                     RKHGLIEPYEQLKAATRGKRIDRDLYSEMLETLDLPASAREELRALTPASYTGLAADL
                     TKREPS"
     gene            complement(5728..5976)
                     /locus_tag="AC16PP_05110"
     CDS             complement(5728..5976)
                     /locus_tag="AC16PP_05110"
                     /inference="COORDINATES: protein motif:HMM:NF016259.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF489 family protein"
                     /protein_id="HIR6481039.1"
                     /translation="MNAPEIDLARRLKGISRLGKRIQVADDPDLLKRESVADTIRAEL
                     LAGVRFAWLWQQLGRRRWHLVLRRRQLLVALETLERKT"
     gene            complement(5973..7061)
                     /gene="mnmA"
                     /locus_tag="AC16PP_05115"
     CDS             complement(5973..7061)
                     /gene="mnmA"
                     /locus_tag="AC16PP_05115"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR6481040.1"
                     /translation="MATPRQTKVIVGMSGGVDSSVAALLLLQEGYAVEGLFMKNWEED
                     DGTEYCTAKEDLADAQRVAERLGITLHLANFAAEYWDNVFEHFLAEYDAGRTPNPDVL
                     CNREIKFKQFADYARALGADMIATGHYVRGAMQDGRFVLLKGVDENKDQSYFLQAVPA
                     EELARCLFPLGALTKTEVRAIAAQAGFENSGKKDSTGICFIGERRFRDFLQRYLPSRP
                     GPIKDTQAMTVGEHLGLAYYTLGQRQGLGIGGLAGRPEAPWYVVDKVTDGNTLVVSQS
                     EEDLLSEWLAAGETNWLAPEPELPRQCTAKVRYRQTDQPCLVSHRADGRLLVRFEQPQ
                     RAVTPGQYVCFYDGDVCLGGALIEATGS"
     gene            7163..7639
                     /gene="moaC"
                     /locus_tag="AC16PP_05120"
     CDS             7163..7639
                     /gene="moaC"
                     /locus_tag="AC16PP_05120"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019821998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR6481041.1"
                     /translation="MSKLTHLNAAGEASMVDVSGKSETLRRAVARGIVRMAPETLREI
                     LQSGVPKGDVIAVARVAGIQAAKRCADLIPLCHPLPLTKVALDFSPAGDDGLEVTAEC
                     VVTGQTGVEMEALTAVSVAALTVYDMCKAIDRGMVIESVCLVSKEGGRSGTWERTP"
     gene            7636..7893
                     /locus_tag="AC16PP_05125"
     CDS             7636..7893
                     /locus_tag="AC16PP_05125"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR6481042.1"
                     /translation="MIPVRVLFFASLREAVGREEVFLELPDSARIDDLLASLDDILSA
                     EAMAALRAENVRLAVNQEMLTAPGRLRPNDEVAFLPPVTGG"
     gene            7896..8375
                     /locus_tag="AC16PP_05130"
     CDS             7896..8375
                     /locus_tag="AC16PP_05130"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003316924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030366 - molybdopterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HIR6481043.1"
                     /translation="MHSEVRIQEADFSVAAQWEALRGRASGEVGAVASFVGLVRDRDP
                     DAPIQGLYLEHYPGMTEASIGKILDETASRWPILDIVVIHRVGGLEPGDQIVFVQVAA
                     AHRPAAFAACEFVMDYLKTDAVLWKRERLMEGERWIEATGDDRRRVARWKADPVGDS"
     gene            8501..>8645
                     /locus_tag="AC16PP_05135"
     CDS             8501..>8645
                     /locus_tag="AC16PP_05135"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251313.1"
                     /note="Converts isocitrate to alpha ketoglutarate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIR6481044.1"
                     /translation="MTYQHIEVPADGEKITVNADDSLNIPDNPIIPFIEGDGIGVDIT
                     PVMK"
BASE COUNT         1432 a         2690 c         2861 g         1662 t
ORIGIN      
        1 gatctcgccc gggagcccat cgtgacctac gtgttcggct tcaccggtcg gtcgcgcttg
       61 gacgatgcgt tcgcggcgga aggactcaaa cccaacgtgg ttttcaccgc tacggacgcg
      121 gacgtaatca agacgtacgt tcgccttggc ctcggcgttg gcatcatcgc caccatggcg
      181 ctcgacgacg agttggacga tgacctggtg gccatcgacg cggcccacct gttctcaccc
      241 agcgtcaccc acatcggctt tcgtcggggt acctttctgc ggcgctacat gtacgacttc
      301 atcgagcggt ttgcgccgca cctggatcag cagatggtcg accttgcggc cgcctgcgcg
      361 acctccgcgg agcgggccgc gctgttcgag gatctcgagc ttcccgtgcg ctgacgctcg
      421 agggcctggc cgcgggtcag ccgacgatcc tgacgacgtt ctgggcgtgc aggccgcact
      481 ccttgcgggt ggcttcttcc caccaccacc gtccggagcg ctcgtgctcg tggggcccca
      541 ccggccgcgt gcacggctcg cagccgatgg agacgaagcc gcggtcgtgc agggggttgt
      601 aagggacgcc gtgctcgcgg atgtatgccc agacgtccgc cgacgaccac tgcgccagcg
      661 ggttgaactt gaccagagaa ccgcttctgc cggcaaaggc ggaatcttcc tgctcggtgg
      721 ggacgctggt tctcgtgacg ctctggtcct ggcgctgacc ggtgatccag gcatccagcg
      781 tggacagctt gcgcttcagc ggcttgatct tgcgaacgcc gcagcattcg gagtggccgt
      841 cctcgtagaa gctgaagagt cctttgctct tcaccagctc gcccaccgcc gtgggttcgg
      901 gcatgaccat ctcgatggcc acgccgtagc ggttgcgcac ggattcgatg aagcgatagg
      961 tttcggcgtg gagacggccg gtatccaggc tgaagacgtc cacggccaac cccagctgat
     1021 gggccatgtc gatcagcacg acgtcctcgg cgccgctgaa cgagatggcg atgtggccgg
     1081 gagcaaaacg gccgaccgca tcggcgagca cttcctgggg tctttgatct ggcgcggggc
     1141 ttggctgcat ggtcctggtc gtgtccggga gttggcgcgc agactagcaa tgctcgcgcg
     1201 gcagagaaaa taatctcttg gtatatgctt acgccctcaa tcgagctgga aaacccaacc
     1261 cgtggacgtt ttgtgtttgg atctggaagg ggtgctgatc cccgaaatat ggcaggcggt
     1321 cgcagaccgc accggcatcg aggagctccg aaagaccaca cgggacattc ccgtttacga
     1381 cgatctcatg ggcatgcggc tcgaggtgct cggcgcaaac gacctgtcca tgagcctcat
     1441 cgaatccgtc atcgacggcc tggagccgct gccgggcgct tcagactttc tggggtgggc
     1501 cagggcgcgc taccaggtgg ccatcatctc ggacacgttt taccagttcg ccaacccgct
     1561 gatggccaag ctcggctggc cgacgctgct gtgtcacaac ctggtggtag agaacgatcg
     1621 aatcgtcggg taccggattc gccagccgga tcccaagcga tgctcggttc aggctttcaa
     1681 gtctctcaac taccgggtgc tggccgccgg ggactcctac aacgacatcg ccatgctgga
     1741 ggaagcagac gcggcgttct tttatcgggc ccccgagaac gtgctggcgg agtatccgca
     1801 gtacccgtgc gcggggtctt acggggagct gcaggcgctt ttaaaggctg cggcctgacg
     1861 gcccgatcgc ttcaacgggt ggcgcgctgc gccgccgggc attgcgcccg gcagcgacgg
     1921 ccgtgtgtca gtggaactga gcttcttccg ttgaaccggt gagagcggtc accgagctct
     1981 gcccgccctg aatgacggtg gcgacctggt cgaagtagcc ggtgccgact tcctgctggt
     2041 ggctgacgaa cgtgtacccc ctgtcggcgg cggcaaactc ttcctcctgg agctccacgt
     2101 aggcggacat ctgcctgctc gcatacccct gcgccaggtt gaacatgttg aaccacatgc
     2161 tgtgaatgcc tgccagcgtg atgaactgat acttgtaacc catggccgcc agctctttct
     2221 ggaacttggc gatggtcgcg tcgtccaggt tggccctcca gttgaaggac ggcgagcagt
     2281 tgtacgacaa catctggtcg gggtattcct tctttatcgc ctcggcgaac cgcttggcct
     2341 cgtccagatc cggtaccgcg gtttcgcacc acagcagatc ggcgtacggc gcgtaggcca
     2401 ggccgcgaga gatggcctga tcaaggcccg cgtgggtctt gtagaaccct tccggcgtgc
     2461 gctctcctgt cagaaaatcc ttgtcccagg ggtcgatgtc gctggttacc atgtcggcgg
     2521 cgtttgcgtc cgtgcgcgcg agcagtatgg tcggcacgtc gcacacgtcg gcggccagac
     2581 gtgcggcgat cagcttctgc accgcctcct gggtgggtac cagcaccttg ccgcccatgt
     2641 ggccgcactt cttcaccgag gccagctggt cctcgaagtg cacgccggca gccccggcgc
     2701 caatcatgga tttcatgagc tcgaaggcgt tgaggatgcc gccgaagccc gcctcggcat
     2761 cggcgatgat cggtgcgaag tagtcgatgc cgtcgtcgat tcctttcgac cattgaatct
     2821 cgtcggcccg ccggaacgcg ttgttgatcc ggttgaccag ggtaggaacg gagttgaccg
     2881 cgtagaggga ttggtccggg tacatggtct ccgagtcgtt ggcgtccgcg gccacctgcc
     2941 agcctgaaca gtagatggcc ttgatgcccg ccttgacctg ctgaacggcc tggccgccgg
     3001 tgacggcgcc gagagagttg atgaagtcct cgttctgcag gtagtcccag agcttgtccg
     3061 cgccgtgagc ggccagcatg ctgcggtggg gtatggtgcc cctcagattg acgacgtcgg
     3121 ccgccgtgta accgcgcttg acgtctttcc agcgcggatt ggtgtcccac tccgtctgca
     3181 attctctgac ctgctcttcg cggggcttcc gcggaatgaa atgtagcgcc atggatcgtt
     3241 cgcccgtctc aaggaatgtc ggggccggga ttgtagagac gcagattgaa aagaatagcg
     3301 aaaggaaaac aattaatgct ttcccaatgg gaatatatca gcgacgtgaa cgacggcgcc
     3361 ggttgcccgt ctcagatacg caggatacgc agctccggat cggggctgct gaggttccac
     3421 aggtagtcga accgctcggt gagctttgat gcctggacgg gatcgtagag gttggtcaga
     3481 ccgtcatact cccggtagtc gggcatcagc cagtagccgc gctggtccca caccagatag
     3541 gtgtgaacgt ccgtggcgag cccgtctggc accttacgga tttcaatctt gctgtcgatc
     3601 cgtcgggcga gctccaggag cttgtggccg cggctcacca tgggctggct ggaatggatg
     3661 aggattctca acgtggcagg cggcgcgctg cgaacgaacc tcgagaccgc atcgacgatc
     3721 tcgggccggt cgaacagcga gtggtccaga tactggctgt agatcagcag cgccctggtc
     3781 ggctcggcca cgcacgcggc gagggcatcg atgctctccg gctcgccatg caggtgcttg
     3841 agctcggccc tcgcatcgtc tgcgtcgctc tcctgaggga cgatcagggg agcgggaatc
     3901 ggctcggcgc ttggcctgaa ctccacgggc aggatcaaac gcatggcgat gtgcggtatg
     3961 tccgcttcca tgaattcctg cccttcgggc acgaacccgg cttcgcggta gaagcgggca
     4021 acctcggtct gggcgtgaac gaagacctcg gcgtggccgt cctcgcacgc tttctccacg
     4081 gccgcctcca ggagccttcg gccgatcccg ccgcgacgct gggacgccac caccgccatt
     4141 cgcccgatct ggccggtacc gaggagcctg gcgcagccca gcgcctggcc cgcttcgttg
     4201 acggcgagaa agtgcgtggc gtctgcatcg agaccgtccc actccagttc ctggggcacc
     4261 ccctgttccc tgacgaagac ggcgtcgcgg acctcgttga gctggtgctg gtgggtcgac
     4321 caatccacgg gcacgacgtg aatgtccatc agctgggttc tctcttggtg aggtcggcgg
     4381 ccaggccggt gtaggaagcc ggcgtgagcg cgcgcagctc ttccctggcg ctcgcgggca
     4441 gatcgagggt ctcgagcatc tcgctgtaca gatcgcgatc gatccgcttg ccgcgggtgg
     4501 cggctttcag ctgctcgtag ggctcgatca ggccgtgctt gcgcatgacg gtctgtacgg
     4561 cctccgccag aacttcccag ctggcgtcca ggtcggcccg gatccggtca ccgtttatct
     4621 tcacgcggcc gaggccgcgc tggctcgcct ggtaggcgac gctgcagtgg ccgcaggcag
     4681 aaccgacgtt gcgaagcacg gttgaatccg acaggtcccg ttgccagcgc gagacgggca
     4741 gcttggcggc catgtgctcg agcagggcgt tggcgatgcc caggttgccc tcggagttct
     4801 cgaagtcgat ggggttgacc ttgtgtggca tggtggacga gccggtttcg ccggcgaccg
     4861 gctgctggac gaagtactcg atggcgatgt agccccacat gtcgcggtcg aagtcgagca
     4921 gtatctgatt gaatctgacc agcgcgtgaa agagctcgga gatgtagtcg tggggttcga
     4981 tctgggtagt cagcgggttc tgcgcgaggc ctaggccttc aacgaaagcc ctcgccagct
     5041 cgggccagtc gacttcggga taggccacca cgtgagcgtt gtagttgccg accgccccgt
     5101 tcatcttgcc cagcagggcc acctcacgca gctgcctgag ctgacgggac aggcggtaag
     5161 cgacgttttt gagctccttg cccatggtgg tgggcgaggc cgcctgaccg tgggttcggg
     5221 acagcatcgg gatctcggcc gcgtcgtcgg cgaccgcgct gatctgatcg atgagcgcct
     5281 gcatctgctc gccgagcagc tcgcgagagt cgcgcagcat cagggcgtaa gagagattgt
     5341 tgacgtcctc cgacgtgcag gcgaagtgga cgaactctat gtgcgaggcg agcccgtcga
     5401 tagccgcgac gcgctccttg acgaagtact ccacggcctt cacgtcgtga ttcgtggtcc
     5461 gctcgatcgc cttgatgcgt tcggcgtccg cgagcgaaaa gtcttccgcc acggcggcaa
     5521 gggcagcggc ctggccggaa gatagggcgg gcagctcgcc gattgaatcg gtctgggcga
     5581 gatgagcgaa ccagcggcac tccaccagca cgcggtagcg catcagtccg aactcgctta
     5641 ccagcggtgc cagtggttcg aggcggttcc ggtagcgccc gtccagggcg ccgatggtca
     5701 tgagcgggtg tgtctgcacg gaggtggtca cgttttcctc tcgagagttt ccaaggctac
     5761 cagcagctgc cggcgtcgca aaaccagatg ccaccggcgg cggcccagct gctgccacag
     5821 ccaggcgaac cggacgccgg caagcagctc ggcgcggatc gtatcggcca cgctttcacg
     5881 cttgagcaga tcgggatcgt cggcgacctg gatgcgcttg ccgagccggc tgatgccctt
     5941 tagccttcgg gccaggtcga tctcgggcgc gttcatgagc ccgtcgcctc gatcagcgcg
     6001 ccgcccaggc aaacgtcgcc gtcgtagaaa cacacgtact ggcccggggt aactgcccgc
     6061 tggggttgct cgaagcgcac cagaaggcga ccgtcggctc ggtggctgac caggcagggt
     6121 tggtccgtct gacggtagcg gaccttcgcc gtgcattgcc tgggaagctc gggctccggt
     6181 gccagccagt tcgtctcgcc ggccgccagc cactcgctga gcaggtcctc ttcgctctga
     6241 gacactacga gcgtgttgcc gtccgtgacc ttgtccacca cgtaccacgg cgcttcggga
     6301 cgtcctgcca ggccgccgat gcccagcccc tgacgctggc ccagggtgta gtaggcgagg
     6361 cccaggtgct cgcccacggt cattgcctgg gtgtccttga tggggcccgg ccggcttggg
     6421 agatagcgtt gcaggaagtc ccggaagcgt cgctcaccga tgaagcagat gcccgtgctg
     6481 tccttcttgc ccgagttttc gaatcctgcc tgagcggcaa tggcacgtac ctcggttttg
     6541 gtcagcgcgc caaggggaaa gaggcacctg gcgagctcct cagccgggac ggcctgtaag
     6601 aagtagctct ggtccttgtt ttcatccacg cccttgagca ggacgaagcg gccgtcctgc
     6661 atggcgccac gcacgtagtg ccccgtggcg atcatgtccg cgccgagggc gcgggcgtag
     6721 tctgcgaact gcttgaattt gatctcgcgg ttgcagagca cgtcgggatt cggcgtgcgg
     6781 cccgcgtcgt actcggcgag aaagtgctcg aagacgttgt cccagtactc ggcagcgaag
     6841 ttcgccaggt gcagagttat gcccaggcgt tcggcgaccc gttgggcgtc ggccagatcc
     6901 tctttggccg tgcagtactc ggtgccgtcg tcctcttccc agttcttcat gaacaggccc
     6961 tccacggcgt acccctcctg caggagcagg agggcggcga cggaggagtc gacaccgccc
     7021 gacatgccga cgatgacttt ggtttgcctg ggagttgcca cttcaggggt gcgtgcagca
     7081 ctgctgtgtg aaggatggcc attctacccc caagcagggg ttcttgcccg cccgacagcc
     7141 cgttgggggc tataatgcga ccatgagcaa gcttacccat ctcaatgccg cgggcgaggc
     7201 gagcatggtc gacgtctccg gcaagtccga gacgctgcgg cgcgccgtgg cccgggggat
     7261 cgtgcggatg gcgccagaga cccttcgcga gatcctgcag tcgggggttc ccaagggtga
     7321 cgtcattgcg gtcgcccgcg tagccggcat tcaggccgcc aaacggtgcg cggatctcat
     7381 cccgctttgc catccgttgc cgctcaccaa ggtggctctg gatttcagcc cggccggcga
     7441 cgacggtctc gaggttaccg ccgagtgcgt ggtcacgggc cagaccgggg tcgagatgga
     7501 agccctgacc gccgtcagcg tggctgcgct gacggtctat gacatgtgca aagccatcga
     7561 ccggggcatg gtcatcgagt ccgtgtgcct ggtgagcaaa gaaggcggcc gcagcggcac
     7621 ctgggaacgc acgccgtgat tcccgtccgt gttctgtttt tcgcatctct cagggaagcc
     7681 gtcggtcgcg aggaggtctt cctcgagctg ccggattcgg cgcggatcga cgacctgctc
     7741 gcctcgctgg atgacattct cagcgccgag gcgatggcag cgctgcgggc ggaaaacgtt
     7801 cgcctggccg tcaaccagga gatgctcacg gcccctgggc ggcttcgtcc caacgatgag
     7861 gtcgccttcc tcccgccggt aacggggggc tgaccgtgca ttccgaggtt cgaattcagg
     7921 aagcggattt cagcgtggcg gcacagtggg aggcgctgcg cgggcgcgcg tcgggcgagg
     7981 ttggcgcggt agcgagcttt gtcgggctcg tgagggaccg cgatcctgat gcgcccatcc
     8041 agggtctgta tctcgagcat tatcccggca tgacggaagc gagcatcggt aagattctcg
     8101 acgagaccgc atcacgctgg cccatcctgg acatcgtggt cattcaccgc gtgggtggcc
     8161 tcgaacccgg cgaccagatc gtgttcgtgc aggtggcggc cgcgcaccgc ccggcagcct
     8221 tcgctgcctg cgagttcgtc atggactatc tgaaaaccga cgccgtgctg tggaagcggg
     8281 agcggctgat ggaaggcgag cgctggatcg aggcgaccgg ggacgaccgt cgccgtgtcg
     8341 cccgatggaa ggccgacccg gtcggcgatt cctgataccc actgcacagc ctcgtcggaa
     8401 gggctataat ctcgcgcctt tttccggtgc gcctttccat cgcgacctat cgcgacgggt
     8461 caaggctgtt acccaaacgg atttccaaac ggagctttag atgacctatc agcacatcga
     8521 agtgcccgct gacggcgaga agatcaccgt caacgcagat gattcactga acatccccga
     8581 caacccgatc atccctttca tcgaggggga cggcatcggg gtcgacatca ccccggtcat
     8641 gaagg
//
LOCUS       DBNHKV010000159         4308 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1367485, whole genome shotgun sequence.
ACCESSION   DBNHKV010000159 DBNHKV010000000
VERSION     DBNHKV010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1367485"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="AC16PP_05140"
     CDS             complement(<1..201)
                     /locus_tag="AC16PP_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR6481045.1"
                     /translation="MNGAQSLLKTFVNAGVEVCFTNPGTSEMHFVAALDTEDGMRCVL
                     GLFEGVVTGAADGYARMAGKPAC"
     gene            332..1684
                     /gene="gorA"
                     /locus_tag="AC16PP_05145"
     CDS             332..1684
                     /gene="gorA"
                     /locus_tag="AC16PP_05145"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016561825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HIR6481046.1"
                     /translation="MSYDYDLFVIGAGSGGVRAARMSAGYGARVAVAESTYLGGTCVN
                     VGCVPKKLFVYGSHFHEDFEDAAAYGWDVSPTGFDWPRLRDNKTREIERLNGVYANLL
                     EKAGVETKWGHARIAGPNTVAMDGQTFTAEHILIATGSWPAVPDVPGRELAITSNEAF
                     YLESFPKRVVVVGGGYIAVEFAGIFKGLGANVELLYRRDLFLRGFDDGVRQFVANELT
                     QKDIGLRFNTNVARIEAVDGRRRVHLVEGGSLDADLVLYATGRTPKTGDLGLETVDVA
                     LNERGGIVVDEFYRTSVPGIYALGDVIDNVQLTPVAIAEGMCVSANLFTDRPASRVNY
                     ENIPTAVFCQPNIGTVGPTETEARERYPDLAVFESEFRPMKHTLTGRQERTFMKLLVD
                     GASDRVVGAHMVGPEAGEIVQGIGVAITAGATKAHFDQTIGIHPTAAEEFVTMREPAR
                     "
     gene            1714..3165
                     /locus_tag="AC16PP_05150"
     CDS             1714..3165
                     /locus_tag="AC16PP_05150"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HIR6481047.1"
                     /translation="MKRMTSFRTRWPRWLAATSLTALAACATMPEQEPASRNVILFLG
                     DGMGVSTVTAARIFEGQRQGLAGEEHALAFEHFPHVALVKTYNTDGQVPDSAGTMSAI
                     MTGEKTRVGHIAVGPEVDNDDCPGSLAGKRQTLLEEAELRGYATGIVSTARITHATPA
                     ATYAHVPNRNWEADSMLPAPASAAGCRDIAAQLVEFEPGDGIDVILGGGRAAFRPAEA
                     PDPEYPDTRGMRADQTNLVERWSAGAPNRRFVWNAEQLDALDPAANEQVLGLFEPSHM
                     QFEADRARDAGGEPSLAELTRFAVERLQGRGPGYFLMIEGGRIDHAHHFTNAYRALVD
                     TLALSDAVATAMAMTDPADTLILVTADHSHTLTISGYPKRGNPILGKVETLAGTPGLD
                     AQGRPYTTLGYANGPSHAEPIRDLTEVDTTDPGFQQPAGVPLDVETHSGEDVAAYAIG
                     LNAEALGGVIEQNVLYGVMREALFGGRKQKQKP"
     gene            3310..>4308
                     /locus_tag="AC16PP_05155"
     CDS             3310..>4308
                     /locus_tag="AC16PP_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR6481048.1"
                     /translation="MYLGGPAALFWMWATAFLGMTTKFVEVTISHRYRVKDAQGRMAG
                     GPMYYMERGLNLRWLAVFFAAATVVSSFGSGNLPQSNNMASGLEASFGIPTWVAGAVL
                     AALLGLVIIGGIRRIAVVAASIVPVMGVLYALGALSVAASNADQIVPSFAAVFSNAFT
                     GSAATGGFLGATFAYAFNRGVNRGLFSNEAGQGSAPIAHASAGTYKEGNPAHVKALFD
                     HLDIVPPANDPVTAFSGSVLVSDGVASSVAFTVLHNRSIAEDVRFQSAGRPFSGVLEV
                     VDGSLVTPDVDIRGRSLLHSVPLTAEAFKRSFLGEYGQYAVTVGLVMFAFSTALAWS"
BASE COUNT          720 a         1416 c         1449 g          723 t
ORIGIN      
        1 gcatgccggc ttgccggcca tgcgggcgta cccgtccgcc gctccggtga ccactccctc
       61 gaacagcccg agcacgcagc gcatgccgtc ctccgtgtcc agcgcggcaa cgaagtgcat
      121 ctccgaggtt cccggattgg tgaagcagac ttccacgcct gcgttcacga acgtcttcaa
      181 caagctctgc gcgccgttca tggccgtacc ggtgacgaga ggaaagcgga gtgtatgtgg
      241 gccggaagaa gcattgcaat tcggtgctca tcaattacgc tccgtcacat cccgcatctg
      301 cgggtccgtt tccccgggga gaatcaagac gatgagctac gactacgacc tgttcgtgat
      361 cggcgcggga tccggcggcg tcagagccgc gcggatgtcg gccggatacg gcgcccgggt
      421 ggccgtagcc gagagcacct acctgggcgg cacgtgcgtc aacgtgggct gcgtgcccaa
      481 gaagctcttc gtctacggct cccactttca cgaggatttc gaggacgccg cggcgtacgg
      541 ctgggacgtt tcgcccacgg gattcgactg gccgaggctg cgcgacaaca agacgcggga
      601 gatcgagcgg ctcaacggcg tgtacgcgaa cctgctcgag aaggcgggcg tggaaaccaa
      661 atggggccat gcgcgaatcg cagggccgaa cacggtggcc atggacgggc agacgttcac
      721 cgccgagcac attctgatcg ccacaggcag ctggcccgcg gtgcccgacg tccccggccg
      781 ggagctggcc atcacctcca acgaagcgtt ctatctggag tcgtttccca aacgcgtggt
      841 ggtggtcggc ggcggctaca tcgcggtgga gtttgccgga atattcaaag gcctcggggc
      901 gaacgtcgag ctgctctacc ggcgcgatct gttcctgcgg ggcttcgacg acggggtgcg
      961 tcagttcgtc gccaacgagc tcacccagaa ggacattggt ctgaggttca acaccaacgt
     1021 ggcccgcatc gaagcggtgg acggccggcg gcgcgtgcac ctggtggagg gtggatctct
     1081 cgacgcggat ctggtgctct acgctaccgg caggaccccg aagacgggcg atctgggcct
     1141 ggaaacggta gatgttgcgc tcaacgagcg tggcggcatc gtggtggacg agttctaccg
     1201 gacttcggtg cccggcatct atgcgcttgg cgacgtgatc gacaacgtgc agctgacgcc
     1261 ggtagccatc gccgagggga tgtgcgtgtc tgccaacctg tttaccgacc ggccggccag
     1321 ccgcgtgaat tacgagaaca ttcccaccgc cgtgttctgc cagccgaaca tcggcacggt
     1381 ggggcccacg gagaccgaag cccgggagcg ctacccggac cttgccgtgt tcgagtcgga
     1441 gttccggccc atgaagcaca cgctgaccgg ccggcaggag cgcacgttca tgaagcttct
     1501 ggtcgacggt gcgagtgacc gcgtcgtcgg tgcgcacatg gtcggtccgg aagctggcga
     1561 gatcgtccag ggcatcggcg tcgccatcac ggcgggtgcg accaaggcgc acttcgacca
     1621 gaccatcggc attcacccca cggcggcgga ggagttcgtc accatgcgcg agcccgcaag
     1681 gtagccgcgg gtgcaggaag cggagcggac ggcatgaaga ggatgacaag tttccggacg
     1741 cgatggccga gatggctcgc tgccacatcc ctcacggcgc tggcggcctg cgcgaccatg
     1801 ccggagcagg aaccggccag ccggaacgtc attctcttcc tcggcgacgg tatgggggtc
     1861 agcaccgtca ccgccgcccg catattcgaa gggcaacgtc agggcctcgc cggcgaggag
     1921 cacgcgctgg ctttcgagca ctttcctcac gtggcgctgg taaagaccta caacacggac
     1981 ggtcaggttc ccgacagcgc cgggaccatg tccgcgatca tgaccgggga gaagacccgc
     2041 gtaggccaca tcgccgtggg gccggaggtc gacaacgacg attgcccggg atctctggcc
     2101 ggcaaacgtc agaccctatt ggaagaggcc gagctgcgcg gctacgcgac gggcatcgtc
     2161 tccacggcca ggatcaccca cgcgacgccg gcggccacct atgcccacgt gcccaaccgg
     2221 aactgggaag ccgacagcat gctgcccgcc ccggcttccg cggccggctg cagggacatc
     2281 gcggcgcagc tggtggaatt cgagccgggc gacggcatcg acgtcatcct gggcggcggt
     2341 cgggccgcct tccgcccggc cgaggcgccg gatcccgagt atcccgacac ccggggaatg
     2401 cgggcggacc agaccaacct ggtggagcgg tggtcggccg gcgcgccgaa ccgccgcttc
     2461 gtctggaatg ccgagcagct ggacgcgctg gatcccgccg ccaacgagca ggtgctggga
     2521 ctgttcgagc cttcccacat gcagttcgag gccgaccggg cccgggatgc cggcggcgaa
     2581 ccgtcacttg ccgagctcac gcggttcgcc gtcgagcgtc tgcagggccg cggccccggc
     2641 tactttctga tgatcgaggg cggccgcatc gaccatgccc atcacttcac gaacgcctat
     2701 cgggcgctcg tggacacgct ggcgctgtcc gacgcggtgg ccactgccat ggccatgacg
     2761 gacccggcgg atacgctcat cctggtgacc gccgaccaca gccacacgct gaccatcagc
     2821 gggtatccga agcgcggcaa ccccatactc ggcaaggtgg agaccctggc gggaacgccc
     2881 ggcctggatg cccagggacg gccttacacc acgctcggct acgccaacgg cccaagccac
     2941 gccgagccga tccgggatct gaccgaggtc gatacaaccg atccgggttt tcagcaaccc
     3001 gccggggtcc cgctggatgt ggagacccac agcggcgagg acgtggccgc ctacgccatc
     3061 ggcctgaacg ccgaagcgtt gggcggggtc atcgagcaga acgtgctgta cggcgtcatg
     3121 cgtgaggcgc tgttcggggg tcggaagcag aagcagaagc catagaaaag ggcggcccca
     3181 tgttcgagga tctcatcggc ttcctggacg gctggctcgg cagcgccgtg tactttccct
     3241 ttcttctgct cggcgtcggg cttttcttca ccctctatct cggtattccc cagattcgat
     3301 tctttcgcca tctacctcgg cgggccggcg gcgcttttct ggatgtgggc cacggccttt
     3361 ctgggcatga ccacgaagtt cgtcgaggtg accatctccc accgctaccg ggtgaaggac
     3421 gcgcaaggac ggatggccgg cgggcccatg tactacatgg agcggggcct caacctgcgc
     3481 tggctggccg tgtttttcgc cgcggccacg gtggtgagct cgttcggatc cggcaacctg
     3541 ccccagagca acaacatggc ctcgggcctg gaggcgtcgt tcggcattcc cacctgggtt
     3601 gccggggcag tgcttgccgc cctgctcggg ctcgtcatca tcggcggtat ccggcgaatc
     3661 gccgtggtgg ctgccagcat cgtgcccgtc atgggggtgc tctacgcgct gggggcgctt
     3721 tcggtggccg caagcaacgc ggatcagatc gttccctcct tcgcagcggt gttctccaac
     3781 gccttcaccg gttcggcggc gacgggcggc ttcctgggcg ccacgtttgc ctacgccttc
     3841 aaccggggcg tcaaccgcgg tttgttctcg aacgaggcgg ggcaggggtc cgcgcccatc
     3901 gcgcacgcct ccgccggcac ctacaaggag ggcaaccctg cccacgtcaa ggcgttgttc
     3961 gatcatctgg acatcgtgcc gccggcgaat gacccggtga cggcgttctc cgggtccgtg
     4021 ctggtgagcg acggggtcgc gtcgagtgtc gcgttcaccg tgctccacaa ccgctccatc
     4081 gcggaagacg tgcgttttca gagcgccggc aggcctttca gcggcgtcct cgaggtcgtc
     4141 gacggaagcc tggtgactcc cgatgtcgac atcagaggcc gatcgctcct gcactccgtg
     4201 ccgctgaccg cagaagcctt caaacgcagt ttcctgggcg agtatgggca gtacgcggtc
     4261 acggtgggcc tggttatgtt cgcgttctcc accgcgctcg cctggtct
//
LOCUS       DBNHKV010000160         3266 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1155896, whole genome shotgun sequence.
ACCESSION   DBNHKV010000160 DBNHKV010000000
VERSION     DBNHKV010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1155896"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1922
                     /locus_tag="AC16PP_05160"
     CDS             <1..1922
                     /locus_tag="AC16PP_05160"
                     /inference="COORDINATES: protein motif:HMM:NF048511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lhr family helicase"
                     /protein_id="HIR6481049.1"
                     /translation="LEDGTIEWTFVSTSSPSPFAANVTFAQINRYMYADDTPEEDAGP
                     SALSDDLIRSAVRNAGLRPPIRREIIQEYLAKRQRTAEGYRPVSAEDWSEWVKERVLI
                     PAAEVRAATSADAPVPEHEHLVWLEERAGSGENRAWLTHRELLHGLAQRGLRDGLAFQ
                     GTVPDVEESRSAEQLALEILSFYGPLTESDIAWILPRVPESLLDENEVLVSGALVADD
                     PGLYYCDAENYAALLRFQRAAARPELQPRPFHTLPGFLASWQRFGVSGGDEHVLEVME
                     PLRGFRAPVRAWLEDVVSARIPAMADSQLDDVLLRHGMQWRGAGRKAIAIGYPEDLQL
                     IDDGVDEPSTLAKLFTDPTARYPFVQLADRSGLSGEAFNEQWWASVWEGEITSDTLAP
                     LRQGLERGYALNAAAPQRASSHRQGRRRARPAPAWTGNWSVKPPFDADIDPLTELEDA
                     KDRARMLLDRYGIICRELANREGRLLRWRCVFRALRLMELAGEVTAGYFFTGLSGPQF
                     VSPGALRRLQQGWQTPEDFWISAVDPASPCALVTAIPDLPARRPNNYLAFHEGSLALV
                     IENAGKRLQFHRGPDDACLGRVCEPLAHLVRRRRRVEVELINDVPARSSPYLEPLGRF
                     FHAVTDHKQLILMIG"
     gene            1972..>3266
                     /locus_tag="AC16PP_05165"
     CDS             1972..>3266
                     /locus_tag="AC16PP_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR6481050.1"
                     /translation="MIVCALIKDEQLRDRLRRLGARAGWRVLFAADDRDLLVTLISGK
                     ATPDVIVSDEPDNLTGVGALPTTRIYLRPDRGEPGTDAFDPALDDQALLQHINNCVNA
                     RRFRARFAAIDRNEPITSLPRHEELFDALSRHVDEPVGMMVVKLDHAEHLYGSLDPVA
                     KTDLLAALSQRLESKLPTQARLGFYDATCFVVTLPRIQSSELERLGNDLRLAVSAPLI
                     DAGGEIHLTISIGCSFASSFNGADHLWASAWAAANAASQAGGDRVLGANPDTAISERI
                     PSALERDEFSLLLQPQWSLQDNRLTAVEALLRWQGRDIGGLAPSHFIPLVEQQGQMAR
                     VGDWVLERACRETAAWLPRMVQPILLTVNVSPQQFHGRSIVTQIARNSAEHRLNPASL
                     ELELSQRNMLRLVDDYREQMYTLRDWGVRFAIDNLGGGL"
BASE COUNT          544 a         1128 c         1057 g          537 t
ORIGIN      
        1 aactggagga cggcaccatc gagtggacgt tcgtgtccac cagctcgccc agccccttcg
       61 cggccaacgt caccttcgcc cagatcaacc gctacatgta cgccgacgac accccggaag
      121 aagacgccgg gcccagcgcg ctgtccgacg atctgatccg cagcgccgtg cgcaacgccg
      181 gcctgcgccc gcccatccgg cgcgaaatca ttcaggagta cctggcgaaa cggcagcgca
      241 cggccgaagg ataccgcccg gtgagcgccg aggactggtc cgaatgggtc aaggagcggg
      301 ttctgattcc ggccgccgag gtccgggccg ccaccagtgc ggacgcgccg gtacccgagc
      361 acgagcacct cgtctggctg gaggagcgcg ccggctccgg agagaaccgc gcctggctca
      421 cccatcggga gttgctccac gggctcgccc aacggggtct gcgggacggc cttgccttcc
      481 aagggacggt cccggacgtc gaggagagcc gaagtgccga gcagctcgcc ctggagatcc
      541 tgtctttcta cggccccttg acggagtccg acatcgcctg gattctgccc cgggttcccg
      601 agagcctgct cgatgagaac gaggtcctcg tgagcggcgc cctggtggca gacgacccgg
      661 ggctgtacta ctgcgatgcg gaaaactacg cggccctgct gcggtttcag cgcgcggcag
      721 ccaggccgga actgcagccc cggcccttcc ataccctgcc cggtttcctg gcaagctggc
      781 agcgcttcgg cgtgagcggc ggggatgagc acgttctcga ggtcatggag cccttgcgcg
      841 ggttccgcgc ccccgtgcgg gcgtggctcg aagacgtcgt ttccgcccgc attccggcaa
      901 tggccgattc ccagctcgac gacgtgctgc tgcgtcacgg catgcagtgg cgcggagccg
      961 ggcgcaaggc gatagccatc ggctatcccg aggatctgca gctcatcgac gacggcgtcg
     1021 atgagccatc aaccctggcc aagctcttca cggatccgac cgcgcgatac ccgtttgtgc
     1081 agctggccga cagaagcgga ctttctggag aagcattcaa cgagcaatgg tgggcgtcgg
     1141 tctgggaagg cgagatcacc tcggacaccc tcgccccgct acggcaggga ctcgaacgcg
     1201 gctacgcctt gaacgccgcg gcgccgcagc gggcctccag ccatcggcag ggacgacggc
     1261 gcgccaggcc ggcgcctgcc tggacgggaa actggtcggt gaagcccccg ttcgacgctg
     1321 acatcgatcc gctcaccgag ctggaggacg ccaaggaccg cgcccgcatg ctgctcgacc
     1381 gatacggcat catctgccgg gagctcgcca atcgcgaagg gcgtctgctg cggtggcgat
     1441 gtgtcttcag ggcactgcgc ctcatggaac tggcaggcga ggtgacggcc ggctactttt
     1501 tcaccggcct gtctggaccc cagttcgtgt cacccggcgc gctgcggcgt ctgcagcagg
     1561 gctggcagac gccggaggat ttctggatca gcgccgtcga tccggcctcg ccctgcgccc
     1621 tcgtcaccgc catacccgac ctgccggccc gccgaccgaa caattatctt gcttttcacg
     1681 agggatccct ggccctggtg atcgaaaatg ccggcaagcg cctgcaattc caccgcgggc
     1741 cggacgatgc ctgcctcggt cgggtctgcg agcccctggc ccatctcgtc cggcgccgca
     1801 ggcgggtgga ggtagagctc atcaacgacg ttcccgcccg ctcgagcccc tacctggaac
     1861 cgttggggcg tttctttcac gctgtgaccg accacaagca attaatcctg atgatcggct
     1921 agcttcggca cttgtgcggg cattttccgc gtgagtttta agaggtcgat cgtgatcgtg
     1981 tgcgccctga tcaaggacga gcagctgcga gaccggttgc ggcggctggg agcccgcgcc
     2041 ggctggcgcg tcctcttcgc ggctgacgat cgcgaccttc tcgtaaccct gatcagcggc
     2101 aaggcaacgc cggacgtcat cgtaagcgac gagccggaca atctgaccgg agtcggcgcg
     2161 ctgcccacga ccaggattta cctgcggccc gacagaggcg agcccggcac ggacgcgttc
     2221 gatcctgctc tggatgacca ggccttgctc cagcacatca acaactgcgt gaatgcccga
     2281 cgcttccggg cccgattcgc cgccatcgat cgcaacgagc cgatcaccag cctgccgcga
     2341 cacgaggagc tgttcgatgc gctgtcgagg catgtcgacg agcccgtggg gatgatggtg
     2401 gtcaagctcg atcatgcgga gcatctctat gggagcctgg atccggtcgc gaagacggac
     2461 cttctcgccg cgctcagcca gaggctggag tcaaagctgc ccactcaggc ccgactcggg
     2521 ttctacgacg ccacctgctt cgtggtcacc ctgcccagaa tccagtcgtc agagctcgag
     2581 cggctgggca acgatcttcg cctggcggtg agcgcaccgc tcatcgacgc gggcggggag
     2641 atccatctga cgatcagcat cgggtgcagc ttcgccagct ccttcaacgg cgccgatcat
     2701 ctgtgggcca gcgcctgggc tgccgccaac gctgcctctc aggctggcgg ggaccgggtc
     2761 ctcggcgcaa accccgacac cgcgatctcc gaacgcatcc cttccgccct ggagcgggac
     2821 gagttctcgc tgctgctgca gccccagtgg agtctgcagg acaaccggct gaccgccgtt
     2881 gaggcgctgc tgcgctggca gggccgcgac atcggcgggc ttgcccccag ccacttcatt
     2941 cccctggtcg agcagcaggg acagatggcc cgggtgggtg actgggtttt agagcgcgcc
     3001 tgccgcgaga ccgccgcgtg gcttccccgc atggtgcagc ccattctgct gacggtaaac
     3061 gtatcgcccc agcagtttca cggccgcagc atcgtcaccc agatcgcccg caacagcgcc
     3121 gagcaccggc tcaaccccgc gtccctggag ctggagctgt cgcaacgcaa catgctgcga
     3181 ctggtggacg actaccgcga gcagatgtat accctgcgcg actggggcgt ccgctttgcc
     3241 atcgacaatc tcggcggcgg gctcat
//
LOCUS       DBNHKV010000161         4493 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1581465, whole genome shotgun sequence.
ACCESSION   DBNHKV010000161 DBNHKV010000000
VERSION     DBNHKV010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1581465"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="AC16PP_05170"
     CDS             complement(<1..1008)
                     /locus_tag="AC16PP_05170"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR6481051.1"
                     /translation="MDASPPLWTWPELCRALSLEVCEGPGVSGIVIDSRKAGPGDLFI
                     ALTGDPGPRFNPSSRSQRDGHDFIDAAVAAGAVGVLAHDGAARDVPQLQVADTLDGLW
                     ALGAAGRARLRCPVVAITGSSGKTTTKGMLAAALGAFSTEGSLNNHLGVPLSLALAPA
                     NASAAVFEIGTNHPGEIAPLSALVRPHVAVVLNVHPAHAENFRDLDELRIEKLSIYSG
                     LKTGSHLVVEDTVSVHDLPDHLSPVRFGRGDGSDVQLLEVRGQTADYLIGGEPFTAHV
                     PGGGEHRAASVAAVLATLKVLDRPVRDGLQLGDDLVPKGRGNETLINGVTVVDDSYNA
                     NP"
     gene            complement(1016..2641)
                     /locus_tag="AC16PP_05175"
     CDS             complement(1016..2641)
                     /locus_tag="AC16PP_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6481052.1"
                     /translation="MANLYENDLDANAANFAALTPLTFLARARDIYPHRTAVIYGERR
                     YTWSEHYERCVRVSDALRRRGVGSGDTVAILAGNIPEMLEAHFAVPMAGAVLNAINVR
                     LDAPTVRFILEHGQAAVLLVDEAFGELASHALEGMATVPLVVGIEDSSLEDGRLIGAA
                     TWNELVAEGSSEATWALPGDEWAAITLNYTSGTTGNPKGVVYHHRGAYLNAVSNLLTW
                     NMGDHPTYLWTLPMFHCNGWCFPWTLAVNAGTSVCVRQVRAEEIFDAIARYGVSHFCG
                     APVVLNTLLSADDGLKAKVTHQVNVMTAGAAPPAAVIEGMEAMGMSVTHVYGLTETYG
                     PVTLCAWQGERWDDLSPSERAALKARQGVRGHMLEGLMVADPETLVPVPMDGATLGEV
                     MMRGNNVMKGYLKNPDATAQSFEGGWFHSGDLAVWHPDGYIQIKDRSKDIIISGGENI
                     SSIEIEDTLYRHPKVREAAVVAKSDARWGETPCAFVELKPDVAASAEEIIDFCRENMA
                     RFKAPKHVVFGELPKTSTGKVQKFVLRERAEQI"
     gene            2749..>4493
                     /gene="sppA"
                     /locus_tag="AC16PP_05180"
     CDS             2749..>4493
                     /gene="sppA"
                     /locus_tag="AC16PP_05180"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIR6481053.1"
                     /translation="MSEPGLMSRIGSWLTRARNFTANLIFVLLVVLFVTVILTSGERI
                     RVPEGAALVLNPTGIVVDKETLGDAIAELLAPESTRRETELAGLLTALETSAGDDRIK
                     TLVLDLTELDFVSTAHAATLGEAIRTFSDAGKEVIAYAHYFTQPQYLVASYADAVYMH
                     PDGQILPIGYEVNRLFFSEMLNKLDINVHIFRVGKYKEFVEPYTRSDMSDEAREANQK
                     LVDGLWRHYSETVTANREIEAQSFESYAEQYSPLLKAAAGDMGRLALEFHLVDELLTP
                     DQTRARIAATVGYAREGEFNGIGYRDYLTARDSDPARSGGANVGVITTQGPIVMGSDG
                     RGVIAADSTVDLIRRARMDDSVSALVVRVNSPGGSAFASELIRQELELTQLAGKPVVV
                     SMSSVAASGGYWIASTANRIFAHPTTITGSIGIFAVIPTFEESLARIGVTSDGVGSSA
                     LSGSLDLSSGIAEPMAAVLQASVERGYEQFLNLVARGRDMTPEAVDEIAQGRVWIGST
                     ALELGLIDELGGVGEAVEAAAGLAGLEAYGVKQIEEPLSARAMLLKQLGESVEIDLFP
                     EPPQVFALLERGASL"
BASE COUNT          813 a         1496 c         1408 g          776 t
ORIGIN      
        1 ggggttggcg ttgtagctgt cgtcgaccac cgtcacgccg ttgatgagcg tttcgttgcc
       61 acggcccttc ggcaccaggt cgtcacccag ctgcaggcca tcgcgcaccg ggcgatccag
      121 aactttcaga gtggccagca cggcggcaac ggacgccgcg cgatgctcgc caccgccggg
      181 cacgtgagcg gtgaagggct cgcccccgat caggtagtcg gccgtctgtc cgcgcacctc
      241 gagcagctgc acgtcagacc cgtctccacg gccaaaacgc accggcgaga gatggtcggg
      301 caggtcgtga acggagacgg tatcttcaac tacgaggtga gatcctgtct ttaacccact
      361 ataaatgctc agcttttcaa tcctcaactc gtccagatcc cggaaatttt ccgcgtgggc
      421 cgggtgaacg ttgagcacga cggcaacgtg gggccgcacc agagcagaaa gcggcgcgat
      481 ctctccggga tggttcgtcc cgatctcgaa cacggccgcg ctggcattcg cgggagccaa
      541 cgcaagagac agcggcacgc ccaggtgatt gttgagactg ccctcggtgg agaatgctcc
      601 cagagcggcc gccagcatgc ccttggtagt ggtcttgccg ctgctgccgg tgatggcgac
      661 cacggggcat ctcaggcgcg ctcgtccagc cgcacccaga gcccacaagc cgtccagcgt
      721 gtccgccacc tgcagttgcg gcacatcgcg cgctgcgccg tcatgggcca ggacgcccac
      781 cgcgccggcg gcgacggccg catcgatgaa gtcgtggcca tcacgctgcg agcggctgct
      841 gggattgaat cgaggtccgg gatccccggt gagcgcgatg aaaagatccc ccggacccgc
      901 cttgcgcgaa tcgatcacga tgcccgacac gccgggacct tcgcaaacct ccaggctcag
      961 ggccctgcac agctccggcc aggtccaaag cgggggcgat gcgtccacgc ccacctcaga
     1021 tctgctccgc ccgttctctg agcacgaatt tctggacctt gcctgtggac gtcttgggca
     1081 gctcgccaaa caccacgtgc ttgggggcct tgaaccgcgc catgttctcc cggcagaaat
     1141 cgatgatctc ctcggcgctt gctgccacat cgggcttgag ctccacgaac gcgcacggcg
     1201 tctcgcccca cctggcgtca ctttttgcca ccaccgccgc ctcgcgcacc ttcgggtggc
     1261 ggtaaagcgt gtcctcaatc tcgatgctgg agatgttctc gcccccggag atgatgatgt
     1321 ccttcgagcg gtctttgatc tggatgtagc cgtccgggtg ccagacggcc aggtcaccgg
     1381 agtgaaacca ccctccctcg aaggattgag cggtggcatc ggggttcttc aggtaaccct
     1441 tcatgacgtt gttgccccgc atcatcacct cgcccagggt cgcgccgtcc ataggcaccg
     1501 gcaccagggt ttccggatct gccaccatca gcccttccag catgtgcccg cgcacgccct
     1561 gccgggcttt cagggccgcc cgctcggatg gtgacaggtc gtcccagcgc tcgccctgcc
     1621 aggcgcaaag ggtgacgggc ccgtaggtct cggtgaggcc gtacacgtgg gtcaccgaca
     1681 tgcccatggc ctccatgcct tcgattaccg cggccggagg cgccgccccg gcggtcatga
     1741 cattgacctg gtgcgtgacc ttcgctttga gcccgtcatc ggcgctcaac aacgtgttca
     1801 gcaccaccgg cgcgccgcag aagtgcgaga ccccgtagcg ggcaatggcg tcgaatatct
     1861 cttccgcgcg tacctgtctc acgcagaccg aggtgccggc attgaccgcc agggtccagg
     1921 ggaaacacca gccgttgcaa tgaaacatcg gcagcgtcca cagataggtc ggatggtccc
     1981 ccatgttcca cgtgagcaaa ttggaaacgg cgttcaggta tgcgccgcgg tggtgataca
     2041 ccaccccttt gggattgccc gtggtgccgg acgtgtagtt gagggtgatg gccgcccact
     2101 cgtcaccagg cagcgcccag gtcgcctcag acgaaccctc ggccacgagc tcgttccacg
     2161 tcgctgcacc aatgagccgg ccgtcctcga ggctcgagtc ttcaatgccc accaccaggg
     2221 gcacggtcgc catgccctcc agcgcgtggg aagccagctc gccgaacgcc tcgtccacca
     2281 gcagcacggc ggcctggccg tgctcgagga tgaagcgcac cgtcggcgca tcgagcctga
     2341 cgttgatggc attgagcacc gcgcccgcca tgggaaccgc gaagtgcgcc tccagcatct
     2401 cgggaatgtt gcccgccagg atcgcgaccg tatcgccgga gcccacgcca cgccgtctca
     2461 gggcgtctga cacccgcacg cagcgctcgt agtgctcgga ccaggtgtaa cgccgctcgc
     2521 cgtagatgac ggccgtccgg tggggataga tatcgcgtgc ccgtgccagg aacgtcagcg
     2581 gggtgagcgc cgcaaagttg gccgcattcg catccagatc gttttcgtag agattggcca
     2641 tgtgcgtggg cgccggcggg aaattctcac ttcagcacag ggcaccgcct agctcaagca
     2701 cgtccagcat gagatgcttg aaagatctaa gcaatccgga gccaccccat gagcgaaccg
     2761 ggcctgatgt cccgcatcgg cagctggttg acccgcgcgc gcaacttcac tgccaacctg
     2821 atattcgtgc tcctcgtcgt tctctttgtg acggtgatcc tcacctccgg agagcgcatc
     2881 cgcgtaccgg aaggggccgc cctggtgctc aatcccaccg gcatcgtcgt ggacaaggag
     2941 acgctgggcg atgcgattgc cgagctgctc gcacccgaat cgacgcgccg ggaaacggag
     3001 ctcgccggcc tgctgaccgc cctggaaaca agcgccgggg acgaccggat caaaaccctg
     3061 gtgctggatc tcaccgagct cgacttcgtc tccacggccc acgccgcgac cctgggtgag
     3121 gcgatcagga cgttcagcga cgctggcaag gaagtgatcg cctacgcgca ctacttcacc
     3181 cagccccagt acctggtcgc cagctacgcc gatgcggttt acatgcatcc cgatggtcag
     3241 atactcccaa ttggctatga agtaaaccgc ctgtttttca gtgagatgct gaacaaactt
     3301 gatataaacg ttcatatatt ccgcgttgga aagtataaag agttcgtgga gccgtatacc
     3361 cgcagcgaca tgtccgacga agcccgcgaa gccaaccaga aactggtgga cggtttgtgg
     3421 cgccactact cggaaacggt gaccgccaac cgggagatcg aggctcagag cttcgagagc
     3481 tacgcggagc aatacagccc cctgctgaag gcggctgccg gtgacatggg acggctggcg
     3541 ctggagttcc atctcgtcga tgagctcctc actccggatc agacccgtgc ccgtatcgcg
     3601 gccaccgtcg gctatgcccg cgaaggcgag ttcaacggca tcgggtatcg ggactatctg
     3661 acggcccggg acagcgaccc cgccagatcc ggcggggcca acgtaggcgt gatcacgacc
     3721 cagggcccca tcgtgatggg gtcggacggc cgcggcgtca tcgccgccga cagcaccgtg
     3781 gacctgattc gtcgggccag gatggacgac agcgtgtctg cgctggttgt ccgcgtgaac
     3841 tcgcccggcg gatcggcctt cgcctcagag ctcatccgcc aggagctcga gcttacccag
     3901 cttgccggca agccggtggt ggtctccatg agcagcgtgg cggcgtccgg cggctactgg
     3961 atcgcctcca ccgccaaccg catcttcgct caccccacca ccattaccgg atccatcggc
     4021 atattcgccg tgatcccaac cttcgaggaa agcctggcgc gcatcggcgt gacgtccgac
     4081 ggcgtgggca gctcggctct gtcgggctcg ctcgacctat cgagcggcat cgcagaaccc
     4141 atggccgccg tgctccaggc gagcgtggag cgcggatacg agcagtttct caatctggtg
     4201 gccagaggca gagacatgac gccggaggcg gtggatgaaa ttgcccaggg acgggtatgg
     4261 atcggcagca cggcgctgga gctcggcctg atcgacgagc tgggcggcgt cggcgaagcc
     4321 gtcgaggcgg cggccggtct cgccgggctg gaagcctacg gggtaaagca gatcgaggag
     4381 ccgctgagcg cacgagctat gctgctcaag caattgggtg aaagcgtcga gatcgacctg
     4441 tttccggagc cgccccaggt gttcgccctg ctcgagcgag gcgcttcgct gat
//
LOCUS       DBNHKV010000162        11643 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1653084, whole genome shotgun sequence.
ACCESSION   DBNHKV010000162 DBNHKV010000000
VERSION     DBNHKV010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11643)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11643)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11643
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1653084"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="AC16PP_05185"
     CDS             complement(<1..458)
                     /locus_tag="AC16PP_05185"
                     /inference="COORDINATES: protein motif:HMM:NF016064.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HIR6481054.1"
                     /translation="MSKDYIISWKVAGVALGLLLTLATALVQPLGVSTQFVVTDAMVA
                     HKVAPQFAESNEYLAKYGEKTDWGVGYGWMLFLGMLVGGAVTHLVLRNRQPEADKGSM
                     PAMWVARFGDSHAKRYAAAFFGGVLLLFGARLAGGCTSGHMISGISQLAVG"
     gene            569..1030
                     /locus_tag="AC16PP_05190"
     CDS             569..1030
                     /locus_tag="AC16PP_05190"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR6481055.1"
                     /translation="MLFALTLAAVTSLLSPADDLQHTAARAERLGLPILLYVSRSDCS
                     FCRRFENDVLFPLIRSGEIDGKILIRELEWDRSAPIADFTGTRISAATLAENYDAKLT
                     PTLLFLDHEGKELISRMTGYHESDYASYYLEAAVDLAFQRMSATIQGETGK"
     gene            1027..2271
                     /locus_tag="AC16PP_05195"
     CDS             1027..2271
                     /locus_tag="AC16PP_05195"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HIR6481056.1"
                     /translation="MSLTDQSGLRVRPICLVVLLAFLAACQESDRTQIAVRDLPVPAG
                     ASSQLPHLSKSEDGTVVMSWVEQRHATATLRHARLSDAGWTLPVTVASGDRWFVNWAD
                     FPSVVPIRGRIWAAHWLTKSRGGYYAYDVAMATSRDAGATWSSPSTPHQDGTATEHGF
                     VTLFPWNGDVGVVWLDGRRTAEPPSGSAASKGMTLRGARIGPDGDARDGELIDDLVCD
                     CCQTDAAMGPDGPFVVYRNRTTDEIRDIRLARFVDDAWEADISVGEDGWEIAGCPVNG
                     PAIDVVGNSVAVAWYTEAQGRARVRLSRSRDGGAGFGPPIDVSADRPIGRVDVVQSPD
                     GETFVSWLRRGDDDSAELRLSRLAPDGSVAMETVVARTSAARPSGFPQMISDAHSLIF
                     AWTEPGEDDSRIRSARVELTER"
     gene            complement(2274..3185)
                     /locus_tag="AC16PP_05200"
     CDS             complement(2274..3185)
                     /locus_tag="AC16PP_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481057.1"
                     /translation="MSRGAPIDLARGAVIRLVLGIISVAIAVACDRTMGTARDSDAAV
                     PSGSFSLPATEAVAETITEPARLVELFAALEETGLRVHHVDHEGEAAMLLVEVDEQHF
                     EPVLVGADGRSRTGVTAVEALTDFGLDVVIGSSFVSELHSLTPVGLLQIEGTVISELQ
                     AHGYTRILGIRPAGLGVVPIRAYHRGMFDSAVQAGPGVVEEGRLDISERDLTRPKYYR
                     TFVGLCNGKALLGASQVPMNLHTVGKRLLEYLGETGRTCDEVVNLAGDREVVLALRSA
                     DKRRVAFFGHPLTAKAGLLGFRRRPKD"
     gene            3317..4774
                     /locus_tag="AC16PP_05205"
     CDS             3317..4774
                     /locus_tag="AC16PP_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HIR6481058.1"
                     /translation="MGLDVEIPGLTQQFIGGVWRDSSPDTLVDVISPSTEEVLTRVAA
                     PTVADADRAVAEARAAFDMGPWALMSVEERVEVCARLCDALEARLDQLNRAWAFESGA
                     TIAHGEMINSGAGVAIWRNALEAAASLPWQEDRGDTLIVREPIGTVLGILTFNGPIVL
                     MGMKIIPALLAGCTVIVKHAPESPLTSRLIAEAIREADFPEGVLSVLAADTPVTQHLV
                     GHPDVDMVALTGGTAIGVDVVKRTADRLARTALELGGKSPAIIAEDVDLEDVMATLVD
                     GASGFMGQVCVSLSRILAPRSRYGEVVDAFAERYAALRVGDPFDPATDRGPLAVERAR
                     ERTERYVAGAHQEGARIVTGGRRPPHLDRGWYYEPTLLADVHNGMTVAREEIFGPVTA
                     VIPYEGIDEAVQIANDSPFGLAASVYTRDQDLAMQVARQLRSGSVAVNAAGVSLTQPF
                     GGYKQSGWGRECGPEGILEFTQIKQIVLGASYLDG"
     gene            complement(4789..6132)
                     /locus_tag="AC16PP_05210"
     CDS             complement(4789..6132)
                     /locus_tag="AC16PP_05210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spinster family MFS transporter"
                     /protein_id="HIR6481059.1"
                     /translation="MQSANDDAAEWPSAARGWYATIILLIAFTFSFVDRQVLNLLVEP
                     IRDHFGISDTEVSILQGFAFAVTYVLLSVPVGRMVDRFNRVAIMIGGVLVWSATTISC
                     GLSRTYTQILVSRLGVGAGEASLAPAAWSVLSDYFRPERLSRPISVYLMGPYLGAGIA
                     MIAGAQVLDWTRDVDFVEVPLLGQLAPWQLTFIAVGLPGVLIAALVGTVREPVRHGRK
                     DAELIAPPWRDVLGYLWRHRGIYTSLHLGVPCIVVMLYGLQAWTPTVLVRVYEWDLAQ
                     AGRVYGFIALFAGSAGVLSGPTVARVLERRGYPDYPLRLAVIAAGCATLSMVALPWQS
                     SAEGALVCIALASFFVTLPLALMTSTMQFVTPNEMRGVVAGMYVVTTNVLGLGLGPTL
                     VAASTDFIFADTSALAYSMALVSVVMGPMAVVLLSVGMKPYITRAAELGAQGGKP"
     gene            6222..7931
                     /locus_tag="AC16PP_05215"
     CDS             6222..7931
                     /locus_tag="AC16PP_05215"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6481060.1"
                     /translation="MLDLIIRNGRIIDGTGAPAFSGDLGIVGGRIAAVGSVADDARRE
                     IDAAGRVVAPGFVDVHTHYDAQVFWDGTLSPSCYHGVTTIFGGHCGFSIAPLSTEAAP
                     YLLHMLARVEGMPVASLEAGVPWDWQSFAEYLGKLDGRLGINAGFMAGHSAIRRVVMG
                     ERAVGHQASDAEIRSMADLLDRCLAEGAMGFSSTLSPTHNDADGQPVPSRHASREELI
                     ELARVCGLHEGTSIEFLPEVGTFSEETKQLMSDLSTTAGRPVNWNVLTPGNPEVVASQ
                     LDASDVARRNGGKVVALTVPQPVLLRINLYGGMVFDAMPGWAPLFELPVPERIEALKD
                     PARRRALDASANSGGPFKELAEWRKVLVHATFRPENKQLEGRTIGSIADEQGKEPIDA
                     LFDLAISEGLRTSFMPPAFAGSGDESLWAVRGELWQDERTVIGASDAGAHLDMIDTFA
                     FSTQVLGNGVRKYGVISLEEAVHQITQVPAELFGLRERGRLESGWHADVVVFDPETVA
                     TGPTHVRSDLPAGEPRVYADAVGVDHVIVNGVEIVRDGTHTGALPGTTLRSGLDTQTP
                     RMD"
     gene            complement(7936..8517)
                     /locus_tag="AC16PP_05220"
     CDS             complement(7936..8517)
                     /locus_tag="AC16PP_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIR6481061.1"
                     /translation="MPDLDKTSVGLGERPALLVVDASVAFTDPACALGADFSAEIGVI
                     AQLMALAGESGWPRFFSTVWYESDDEARVFREKVPALNLLDAGSPLVAIDPRLPVDAS
                     DRVFRKTHASCFFGTDLYRWLREASVDGLVIAGFTTSGCVRASAVDALQNDYRTTVIE
                     DAVGDRDPAAHAANLYDIEAKYGDVRSLVSLLA"
     gene            complement(8524..10407)
                     /locus_tag="AC16PP_05225"
     CDS             complement(8524..10407)
                     /locus_tag="AC16PP_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR6481062.1"
                     /translation="MTGRILQTNDAPFTRLDVDPVTLDIIENALRNARVEMDATLFRT
                     AMSPGIREQGDAFPMIANVEGKMVVGQFGSFIHGFMEAFDGVLEEGDMIFTNDPYSCG
                     AAVSHLNDWLVLKPIFKDGRHLCWAAHFGHMTDIGGKVPGSLPIDAREIFEEGVAVPP
                     VKIFRKGELQEDIISIVLRNSRMPHWNKGDFNGVIAACNMAERRCIEIAERFGDDVFY
                     SAMDELLARNCRAMTRLIQDTVPETKQVFEDYICDDGMGMGPYKIRCAMWREGDKVIF
                     DFDGTDPQSSGSINFLLNEEMFKMFCGTFMISVFDPQILFNDGFYDFMEVRIPEGCML
                     KPRRPAALSCRTHALGRIFDVISGLLGQGNPEFLCAAGFSDSPHLMYSGYDKEGEWYQ
                     LFQIGFGGIPGRPMGDGPDGHSLWPGFTNVPNEYIEAYFPLRIETYETIPDSGGPGQN
                     RGGNGIRMGYRFLEDGQISIHDDRWLTYPWGVNGGLPGGRGSKVLVRVDGTQEQLPSK
                     CDRIEVAEGDILYYNTWGGGGAGDPFEREPERVGKDVERGLVTAEGARRYGVVCDAAG
                     VVDADATEQLRSQLRAARQEIPLFDFGGTVDEIKRRCREETGLEPPDAPVFARRGSGL
                     AAG"
     gene            complement(10419..>11643)
                     /locus_tag="AC16PP_05230"
     CDS             complement(10419..>11643)
                     /locus_tag="AC16PP_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR6481063.1"
                     /translation="FDMGGSSTDVSLIEQGKAVVRRETTVGDVNVRASSMDVRTVGAG
                     GGSIAYVPELTGALRVGPDSAGAVPGPAAYDLGGDRPTVTDANVVLGYLPAEQKLGGD
                     MTISRDKAAAAVQQIADAMGLSLEAAAEGIVKIVNENMFGALRLVSVEKGYDPRNFSL
                     IAFGGAGPLHANALGKLTQAWPVIVPPGPGVLCAYGDATTRLRDEASRTLISKLDDIE
                     PAAIADELKQLEALARQAMLAEGVGDAAAQVTDADITAQFQVDVRYSGQGLVLTVDTS
                     EEDLLSDGFAKVSEAFDRLHDQLFTFALDADKEFVNLRVVVEGPEAHVSASELEPGAA
                     DPAAASLGEHQIYADGQTHAGTLYDRARLHPGNVVPGPAVIMEMDSTTLILPGHHAEV
                     DRIGNLLIRPDGHDD"
BASE COUNT         2046 a         3917 c         3785 g         1895 t
ORIGIN      
        1 cccaccgcca gctgcgagat gccggagatc atgtggccgc tggtgcaccc tcctgcaagc
       61 cgcgccccga acaacagcag cacgccgccg aagaaggccg ccgcgtagcg cttcgcgtgg
      121 gaatcaccga accgggcgac ccacatggcc ggcatcgagc ctttatccgc ttccggctgg
      181 cgattgcgga gaacgagatg agtgaccgcg cccccaacca gcatgcccag gaacagcatc
      241 cagccgtagc cgacgcccca atccgtcttc tcgccgtact tcgcgaggta ctcgttggat
      301 tcggcgaatt gcggcgcgac tttgtgcgct accatggcgt ccgtcacgac gaactgggta
      361 gaaacaccga gcggctggac gagggcggtg gcaagggtca gcagcagacc cagcgcgacc
      421 ccggcgactt tccaggaaat gatgtaatcc ttgctcaacg gaggaacctc cacggtgaac
      481 gatgacagga acgtacatta tcattttcgt atattaatga aaactgataa aaggccccct
      541 cttccggggc ggactcagga gtagacgttt gcttttcgca ctcaccctgg cggccgtgac
      601 gagtctcctg tctcccgccg acgacctcca gcatacggct gcccgagccg aacggctcgg
      661 cctgccgatt ctcctgtacg tgtcccgaag cgactgcagt ttctgccggc gcttcgagaa
      721 cgacgtgctg tttcccctga tccgctcggg cgagatcgat gggaaaattc tcatccggga
      781 gctggagtgg gaccgcagcg cgccaatcgc cgacttcacc ggcacacgga tctctgccgc
      841 gacgttggct gaaaactacg atgcaaagct caccccgacc ctcctgtttc tcgaccatga
      901 gggtaaagag ctcatctcac gaatgaccgg ctaccatgag tcggactacg cctcctacta
      961 tctggaggcc gccgtggacc tcgctttcca gcggatgagc gcgaccatac agggggaaac
     1021 cggaaaatga gcctgactga ccaatccggc cttcgagttc gcccgatctg cctcgtcgtc
     1081 ctgctggcct tcctcgcagc ctgccaggaa tccgaccgga cgcagatcgc cgtacgggat
     1141 ctcccggtgc cggcgggggc gagcagccag ctgccccacc tttcgaaaag cgaggacggc
     1201 accgtggtga tgagctgggt cgagcaacga cacgccacag ccacgctgcg ccatgcgcgg
     1261 ctgtccgatg cgggctggac cctgccggtc accgtggcca gcggtgaccg atggttcgtc
     1321 aactgggccg acttcccatc cgtcgttccc atccgtggca ggatctgggc cgcccattgg
     1381 ctgacgaaga gccggggcgg gtactacgcc tatgacgtcg cgatggcgac gtcgcgtgac
     1441 gcgggcgcca cctggtcgtc gcccagcacg ccccatcagg acgggaccgc gaccgagcac
     1501 ggcttcgtga ccctgttccc gtggaatggc gacgtgggcg tggtctggct cgacggccgc
     1561 cggacggccg aacccccgtc gggcagcgca gcatcgaagg ggatgaccct gcgcggcgct
     1621 cgcatcggtc ccgatggcga cgcccgggac ggcgagctga tcgacgacct cgtctgcgat
     1681 tgctgccaga cggacgcggc catgggtccg gatggtcctt tcgtggtcta ccggaaccgg
     1741 acgacggacg agatccggga cattcggctg gcccgctttg tcgatgacgc atgggaggcg
     1801 gatatctccg ttggcgagga cgggtgggag atcgccggct gcccggtcaa cggccccgcc
     1861 atcgatgttg tcggcaactc tgtggccgtc gcctggtaca cggaggccca gggccgggcg
     1921 cgcgtccggc tgagccggtc gagggacggc ggcgcgggct tcggcccgcc aatcgacgtg
     1981 agcgccgacc gccccatcgg gcgcgtggac gtggttcagt ccccggacgg cgaaacgttt
     2041 gtcagctggc tgcgtcgcgg ggacgacgat tccgccgagc tgcgtttgag ccgcctcgcg
     2101 cctgacggct ccgtcgccat ggagacggtc gtcgcccgaa cgtcggcggc ccggccctcg
     2161 gggttcccac agatgatctc cgacgcgcac agcctgattt tcgcgtggac cgaaccgggc
     2221 gaggatgatt cgcgcattcg ctccgcccgg gtcgagctca ccgagcgctg atttcagtcc
     2281 ttcgggcggc ggcgaaaacc gagcaatccg gccttggccg tcagcggatg accgaaaaag
     2341 gcgacccgac gcttatcggc cgaccgcagc gccagaacca cctcgcgatc cccggccagg
     2401 ttgaccacct cgtcacacgt ccgtcccgtt tccccgaggt attcgagcag ccgtttgcca
     2461 acggtatgga ggttcatggg gacctggctg gcgcccagca gggccttacc gttgcaaagg
     2521 cccacgaagg tccggtagta cttgggtctg gtcaggtccc gctcggagat gtcgagcctg
     2581 ccctcctcca cgaccccagg accggcttgc acggcggagt cgaacatgcc gcggtgatag
     2641 gcccggatcg gaaccacacc gagaccggcc ggccggatgc ccagaattcg ggtgtaacca
     2701 tgggcctgca gctcgctgat cacggtgcct tcgatctgga gcaggcccac cggggtgagg
     2761 gagtgcagct cggagacgaa gctgctgccg atgacgacgt ccagcccgaa atcggtgagc
     2821 gcctccacgg cggttacacc cgttcggctg cgcccgtcgg cgccaaccag aacgggctcg
     2881 aagtgctgct cgtccacctc gacaagcagc atggcggctt cgccctcatg atcgacgtgg
     2941 tggacccgca acccggtttc ctcgagcgcc gcgaacagct cgacgagacg tgccggttcg
     3001 gtgatggtct ccgccaccgc ttccgtcgcg ggcagcgaaa agcttccgct gggcaccgca
     3061 gcgtcgctgt cccgcgccgt gcccatcgtc cgatcacagg caacggcaat cgccaccgag
     3121 atgatgccga ggacgaggcg gatcacggcc ccgcgggcca agtcgattgg ggcgcccctg
     3181 ctcacctgat ctgattcccc gtattgcatg ccgacaggcc gccattatgg catcacccgg
     3241 gcacgccgcg cggcccgctg tggcgatcga cgcgcctttg tcgcaaacta actttttcca
     3301 tttgagggaa cgggccatgg ggctagacgt ggaaattcca gggctgacac agcagttcat
     3361 cggcggcgtc tggcgcgatt cttcaccgga cacgctcgtg gacgtcatat ctccttcaac
     3421 cgaggaggtg ctcacacggg tggctgcgcc gacggtggca gacgccgacc gggctgtcgc
     3481 cgaagcgcga gcggcgttcg acatgggacc gtgggccctg atgtcagttg aggaacgcgt
     3541 cgaggtctgt gcacggcttt gcgacgccct cgaggcccgt ctggatcagc tgaatcgcgc
     3601 ctgggcattc gagtcgggcg ccactatcgc tcacggcgag atgatcaact ccggcgccgg
     3661 cgtggccatc tggcgcaacg cgctggaagc ggccgcgtca cttccgtggc aggaagaccg
     3721 gggggatacg ctcatcgtcc gggagcccat cggcacggtt ctcgggattc tgacgttcaa
     3781 cgggcccatc gtgctgatgg gcatgaagat cattcccgcc ctgctggccg ggtgcacggt
     3841 gatcgtgaaa cacgctcccg agtcgcccct gacttccagg ctcatcgccg aggccatccg
     3901 tgaagcggac tttccggagg gcgtgctcag cgtcctggcc gccgacacgc cggtcactca
     3961 gcatctggtg ggccatcccg atgtcgacat ggtggccctg accggcggca cggccatcgg
     4021 cgtagatgtg gtcaaacgca ccgccgacag gctggcccgc accgcgctgg agctcggtgg
     4081 caagtcgccg gccatcatcg ccgaggacgt cgacctggag gacgtcatgg cgaccctcgt
     4141 cgacggcgcg tccggcttca tgggccaggt ttgcgtgagc ctctcgcgca tactcgcgcc
     4201 gcgcagtcgt tacggcgagg tggtcgatgc ctttgccgag cgctacgctg ccttgcgcgt
     4261 gggcgacccc ttcgatcccg ccacagaccg cggcccactg gccgtggagc gggcccggga
     4321 acgcaccgaa cgctacgtcg ccggcgcgca tcaggaaggc gcccggatcg tcaccggcgg
     4381 tcggcggccc ccccacctgg accgcggctg gtactacgag ccgaccctgc tggccgacgt
     4441 ccacaacggc atgacggtgg ccagggaaga gatcttcggc ccggtaaccg cggtcattcc
     4501 ctacgagggc atcgacgaag cggtgcagat cgccaacgac tcacctttcg ggctcgcggc
     4561 gtcggtctat acccgcgacc aggatctggc aatgcaggtg gcgcgccagc tgcgcagcgg
     4621 cagcgtggcc gtgaacgccg ccggcgtctc gctgacccag ccattcggcg ggtacaagca
     4681 gtcgggatgg ggccgcgagt gtggaccgga aggaattctg gagttcaccc agatcaagca
     4741 gatcgtcctc ggcgccagct atctggacgg ctgatctggc aggccggcct acggcttccc
     4801 tccctgcgcg ccgagctccg ccgcacgggt gatgtagggc ttcatgccga cgctcagcag
     4861 caccaccgcc ataggcccca tgacgacaga gaccagcgcc atggaatagg ccagcgccga
     4921 ggtgtctgca aagatgaaat cggtggaagc ggcgacgagc gtgggcccga gccccagacc
     4981 gagcacgttg gtggtaacaa cgtacatccc cgcgacgaca ccgcgcatct cgttgggcgt
     5041 cacgaactgc atagtggacg tcatgagcgc cagaggcaga gtgacgaaga agctcgcaag
     5101 cgcgatgcag accagcgcac cctccgcgct tgactgccag ggcagcgcga ccatgctcag
     5161 cgtcgcgcag ccggcagcga tcacggcaag ccgcagcggg tagtcggggt atccgcgccg
     5221 ttccagaaca cgtgccacgg tgggccccga cagcaccccc gcggaaccgg caaagagcgc
     5281 gatgaagccg tagacccgcc ccgcctgagc cagatcccac tcgtagaccc tcacaagcac
     5341 cgtgggcgtc cacgcctgca gcccgtaaag catcaccacg atgcagggca ccccgagatg
     5401 cagggaggtg tagatgcctc tgtgtcgcca caggtagcca agcacgtccc gccagggcgg
     5461 ggcgatgagc tcggcgtcct tgcgaccatg acggacgggt tcacgcaccg tgccgaccag
     5521 cgccgcgatg agcacgccgg gcagccctac cgcgatgaag gtcagctgcc atggcgccag
     5581 ctggcccagc aaaggcacct ccacgaagtc gacgtcgcgg gtccagtcga gcacctgtgc
     5641 gcctgcgatc atggcaatac cggcgccgag gtacggaccc atcaggtaga cgctgatggg
     5701 gcgcgaaaga cgctccgggc gaaagtagtc cgacagcacg gaccatgccg cgggcgccag
     5761 cgaagcctcg ccagccccca cgcccaggcg cgagacgagt atctgggtgt aggtgcgcga
     5821 cagcccgcag gagatggtgg tcgcgctcca gaccagcacg ccgccgatca tgatggccac
     5881 gcggttgaac cgatccacca tgcgccccac gggaacgctc agaagcacgt aggtgactgc
     5941 gaacgcaaag ccctggagga tgctgacttc cgtatcggag atcccgaagt gatcccgaat
     6001 gggctcgacc aggaggttca gtacctgccg atccacgaaa gagaaagtga aggcgatgag
     6061 caggatgatg gtggcgtacc atccccgcgc ggcggacggc cattcggccg cgtcgtcatt
     6121 ggcagactgc aaaggggccc ctcccgggct tgagctgatc accgggcttt ggtaacgcgc
     6181 cggaaagcta tatcttacgg gctaacaacg ggaggagcga gatgctcgat ctgatcatca
     6241 ggaacggccg catcatcgac ggcaccggcg cgccggcgtt cagcggcgat ctcggcatag
     6301 tcggcggacg catcgccgcg gttggctcgg tcgccgacga cgcgcggcgg gaaatagacg
     6361 cggcgggtcg ggtggtcgcc cccggcttcg tggacgtgca cactcattac gatgcccagg
     6421 tcttctggga cggcacgctg agcccgtcgt gttaccacgg cgtgaccacc atattcggcg
     6481 gccattgcgg tttcagcatc gcgccgctga gcaccgaggc ggccccctac ctgctccaca
     6541 tgctggcccg cgtggaagga atgccggtgg cgagcctgga ggccggagtg ccctgggact
     6601 ggcagagctt tgccgagtat ctcggcaagc tcgatgggcg gctggggatc aacgcgggtt
     6661 tcatggccgg ccacagcgcc atccgccggg tcgtcatggg tgagcgcgcg gtcggccatc
     6721 aagccagcga cgcagagatc cggagcatgg ccgacctgct ggatcgctgt ctggctgaag
     6781 gtgccatggg cttctcctcc acgctctccc caacccacaa cgacgcggac ggtcagcccg
     6841 ttccctcccg gcacgccagt cgcgaagagc tcatcgagct ggcgcgcgtg tgcggcctgc
     6901 acgaggggac cagcatcgag tttctccccg aggtagggac gttctccgag gaaaccaagc
     6961 agctgatgtc ggacctttcg acaaccgcag gccgcccggt caactggaac gtgctcacac
     7021 cgggcaatcc ggaagtcgtg gcaagccagc tcgacgccag cgacgtcgcc agacgcaacg
     7081 gaggcaaggt ggtggcgctc acggttcccc agccggtgct gctcagaatc aacctctacg
     7141 gcggcatggt gttcgacgcg atgcccggct gggcgccgct gttcgagctg cccgttcccg
     7201 agcgcatcga agcgctgaag gatcccgccc ggcgccgcgc gctcgatgcc agcgccaact
     7261 ccggcggccc cttcaaggag ctggccgagt ggcgcaaagt gctcgtgcac gccaccttcc
     7321 gacccgagaa caaacaattg gagggccgca ccatcggctc gattgccgac gagcagggca
     7381 aagagcccat cgacgccctc ttcgacctcg ccatctccga gggcctgcgg acctcgttca
     7441 tgccccccgc tttcgcgggc agcggcgacg agtccctgtg ggccgtccgc ggcgagctct
     7501 ggcaggatga gcgaacggtc atcggcgcct cggacgcggg cgcccatctg gacatgatcg
     7561 acacctttgc cttttcgacc caggtgctcg gcaacggcgt tcgcaaatac ggcgtgatct
     7621 ctctggaaga agccgtccac cagatcaccc aggtgcctgc ggaactgttc ggactgcggg
     7681 agcgcggccg cctggagagc ggctggcatg cggacgtggt cgtgttcgac ccggaaaccg
     7741 tggccactgg cccaacgcat gtgcgatcgg acctgcccgc cggagagccc cgggtctatg
     7801 ccgacgctgt gggcgtggat cacgtgatcg tgaacggtgt cgagatcgtc cgggatggga
     7861 cccataccgg cgccttgccc ggcaccacct tgagatcggg tctggatacc cagacgccgc
     7921 gaatggactg atcgttcagg ccagcaggct gaccagagag cgaacgtcgc cgtacttcgc
     7981 ctcgatgtcg tagaggttgg ccgcgtgggc ggcggggtcc cggtcgccca cggcgtcttc
     8041 aatcaccgtc gttcgatagt cgttctgcag agcgtccacc gccgaggcgc gcacgcaacc
     8101 gctggtggtg aatcccgcta tgacaaggcc gtccaccgaa gcctcccgaa gccagcggta
     8161 cagatcggtg ccgaagaagc aggatgcgtg ggttttgcgg aacaccctgt cggaggcatc
     8221 cacgggcaac ctcgggtcga tggcgaccag ggggctgccc gcatcgagca gattcagagc
     8281 ggggaccttt tcccggaaca cgcgggcttc gtcatcggac tcgtaccaga cggtggagaa
     8341 aaaccggggc cagcccgatt cgccggccag cgccatcagc tgcgcgatga cgccaatctc
     8401 ggccgagaag tcggcgccga gcgcgcacgc ggggtcagtg aaagccacgc tcgcatccac
     8461 cacgagaagg gccggacgct cgcccagacc gaccgacgtc ttgtccaggt caggcacgac
     8521 ctgctacccc gccgccagac cgcttccccg gcgggcaaat accggtgcat ccggcggctc
     8581 cagaccggtc tcctcgcggc atcggcgctt gatctcatcc accgtgccgc caaagtcgaa
     8641 caggggaatc tcctggcgcg ccgcccgcag ctgcgacctg agctgctcgg tggcgtccgc
     8701 atcgacgacc cccgcagcgt cgcagaccac gccgtagcgg cgggcacctt ccgcggtgac
     8761 caggccgcgc tcgacgtcct tgcccacccg ctcgggctca cgctcgaaag gatcacccgc
     8821 gccgccgccg ccccaggtgt tgtagtagag gatgtctcct tcggcgacct cgatccggtc
     8881 gcacttggat ggcagctgtt cctgggtgcc gtcgaccctg accagcacct tgctgccccg
     8941 gccgccgggc agcccgccgt tgactcccca gggataggtg agccagcgat cgtcgtgtat
     9001 ggagatctgg ccgtcttcca ggaaacggta gcccatccgg atcccgttgc cgcccctgtt
     9061 ctggccgggc cctccggagt cagggatcgt ctcgtaggtc tcgatgcgca gcgggaagta
     9121 ggcctcgatg tactcgttgg gaacattcgt gaagccgggc cacagcgagt ggccgtcggg
     9181 cccgtcaccc atgggccggc ccggaatgcc tccgaagccg atctggaaga gctggtacca
     9241 ctcgccctcc ttgtcgtagc ccgagtacat caggtgcggg ctgtcggaga acccggcggc
     9301 gcagaggaac tcgggattgc cctgcccgag caaacccgag atcacgtcga agatgcgtcc
     9361 gagagcgtgc gttcggcaag acagcgccgc gggtcggcgc ggcttgagca tgcagccctc
     9421 ggggatgcgc acctccatga agtcgtagaa gccgtcgttg aagagaatct gagggtcgaa
     9481 caccgagatc atgaacgtcc cgcagaacat cttgaacatc tcttcgttga gcaggaaatt
     9541 gatggagccc gagctctggg gatcggtgcc gtcgaaatcg aagatcacct tgtccccttc
     9601 gcgccacatc gcacagcgga tcttgtaagg ccccatgccc atgccgtcgt cgcagatgta
     9661 gtcctcgaaa acctgcttgg tctccggaac ggtatcctgg atcagccgcg tcattgcccg
     9721 acagttgcgg gccagcaact cgtccatggc agagtagaac acgtcgtcgc cgaaacgctc
     9781 ggcgatctcg atgcagcgcc gctccgccat gttgcaggcg gcgatcaccc cgttgaagtc
     9841 ccctttgttc cagtggggca tgcgcgagtt gcgcaggacg atgctgatga tgtcttcctg
     9901 cagctcgcct ttgcggaata tcttcaccgg cggcaccgcg acgccttcct cgaagatctc
     9961 ccgggcgtcg atgggcagag agcccggcac tttgccgccg atgtcggtca tgtggccgaa
    10021 gtgggcggcc cagcagaggt gccggccgtc tttgaagatc ggcttcagta ccagccagtc
    10081 gttgagatgg ctcaccgcgg cgccgcagct gtacgggtcg ttggtgaata tcatgtcccc
    10141 ttcttcgagc accccgtcga aggcttccat gaacccgtgg atgaacgagc cgaactggcc
    10201 caccaccatc ttcccttcga cgttggcgat catggggaac gcatccccct gctcccggat
    10261 gcccggcgac atggccgtcc ggaacagcgt ggcgtccatt tccacccgag cattgcgaag
    10321 cgcgttctcg atgatgtcca gggtcaccgg gtccacgtcc agccgcgtga acggagcgtc
    10381 gttggtctgt agtattcgtc ctgtcatgtg agcgcccctc agtcgtcgtg cccgtccggg
    10441 cggatgagca gattgcctat acggtcgacc tcggcgtgat gccccggaag gataagagtg
    10501 gtcgagtcca tctccatgat caccgccggc cccggcacca cgttacccgg gtggagtcgc
    10561 gcccggtcgt aaagcgttcc cgcgtgggtc tgcccgtcgg catatatctg gtgctccccc
    10621 agggaggcgg ccgcgggatc ggccgcgccc ggctccaact cggacgcgga aacgtgcgcc
    10681 tccggccctt ccaccaccac acgcagattg acgaactcct tgtccgcatc cagcgcaaag
    10741 gtgaagagct ggtcgtggag ccggtcgaac gcctccgaca ccttcgcgaa cccatctgag
    10801 agcagatcct cttcgctggt atccacggtg agcacgaggc cctggccgct gtagcgaacg
    10861 tcgacctgga actgcgccgt gatgtcggca tcggtgacct gagccgcagc gtccccgacg
    10921 ccttcggcca gcatggcctg ccgggcgagc gcttcgagct gcttgagctc gtcggcaatg
    10981 gccgccggct cgatgtcgtc cagcttggag atcagcgtcc gggaagcttc gtcgcgcagc
    11041 ctggtggtcg cgtcgccata ggcacacagc acgccgggtc ctggcgggac gatcacgggc
    11101 caggcctggg tcagcttgcc cagcgcgttg gcgtgcagcg gtccggcacc gccaaaggcg
    11161 atgagggaaa agttccgggg atcgtaaccc ttctccaccg agaccagccg cagggcgcca
    11221 aacatgttct cgttgacgat cttgacgatg ccttcagccg cggcttcgag ggagagcccc
    11281 atggcgtcag cgatctgctg cacggcggcc gccgctttgt cccggctgat ggtcatgtcg
    11341 ccgccgagct tctgctccgc gggcaggtag ccgagaacca cgttggcatc ggtgaccgtc
    11401 ggccgatcgc cacccagatc ataggcggcc ggcccgggaa ccgccccggc cgaatccgga
    11461 cctacgcgca gcgcgccggt cagctccggc acgtaggcga tggacccgcc gccggcaccc
    11521 actgtgcgca cgtccatgga gcttgcccgg acgttgacgt caccaacggt ggtttccctg
    11581 cgcaccacgg ctttgccctg ctcgatgagc gaaacgtccg tggacgaacc tcccatgtcg
    11641 aag
//
LOCUS       DBNHKV010000163         2848 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_306832, whole genome shotgun sequence.
ACCESSION   DBNHKV010000163 DBNHKV010000000
VERSION     DBNHKV010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_306832"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="AC16PP_05235"
     CDS             complement(<1..506)
                     /locus_tag="AC16PP_05235"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR6481064.1"
                     /translation="METITLVEGLCFGEGPRWRDGRLWLSDMHAQQVIAVDGDGHVQV
                     VVEVPNLPSGLGWLPDGDLLIVSMRDRRVLRHDGARLHPHADLSQLASFDCNDMVVDV
                     HGRAYVGNFGFDLHGGEAPRAAELICVEPDGTARIVADDLLFPNGTVITPDGNTLIVA
                     ESWGGRLTA"
     gene            685..1029
                     /locus_tag="AC16PP_05240"
     CDS             685..1029
                     /locus_tag="AC16PP_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481065.1"
                     /translation="MSTIKNWMMALLGVVVLVGFVGCGQDAGSGPADAPAAAEEAAAD
                     ETAGGFIQPAAEAAEEAMDEGMEALEEAADETATEAAAEAAEMMEEAAAEAEEAAESA
                     MGEMNITPPGSN"
     gene            1318..2502
                     /locus_tag="AC16PP_05245"
     CDS             1318..2502
                     /locus_tag="AC16PP_05245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6481066.1"
                     /translation="MKDVVIVDSVRTGLAKSFRGSFNLTRSDDMLAHCIDALLDRNGG
                     VSPHEVEDVVVGAASHVGEQDGNLARLAVVLSKLPITTAGLTINRFCSSGLQSIAIAA
                     NQIATGQTQVAIAGGVDSISMRTRQEAAKAELNKRLVEEKPDIYMAMGNTAEVVARRY
                     QVTREAQDEYALQSQQRYAAAAEAGYVAEEIVPMAATMLKVDKETGEESRVDVVVDRD
                     ECNRPTTTLEGLASLPPAFEEDGSVTAGNASQLSDGASMTLLMSAERAAQLGLEPLGI
                     YRGFTVAGCEPDEMGIGPVFAIPRLLENAGLKQDDVDLWELNEAFASQCLYCRDALGI
                     DNEIYNVNGGSISIGHPFGMTGSRMTGLVLRELKRRGKRYGVVTMCIGGGQGAAGLFE
                     AA"
BASE COUNT          549 a          854 c          956 g          489 t
ORIGIN      
        1 gcggtcagtc ggccgcccca ggattctgca acgatcagcg tgttgccgtc cggcgtgatg
       61 accgtcccgt tgggaaagag caggtcgtct gcgacgatcc tcgcggttcc gtcaggttcc
      121 acgcagatta gctccgcggc cctgggcgct tctccgccgt gaagatcgaa accgaagttt
      181 cccacgtagg ccctaccgtg gacgtccacc accatgtcgt tgcagtcgaa acttgccagc
      241 tgtgacaggt cagcatgggg gtgcaggcgg gcgccgtcgt ggcgaagaac gcgccgatcg
      301 cgcatggaga cgatcaggag gtcgccgtcc gggagccatc ccaggccgga ggggaggttc
      361 ggcacctcga ccacgacctg aacgtggccg tcgccgtcca cggcgatcac ctgttgagcg
      421 tgcatatcag acagccacag acggccgtct cgccagcgcg gcccctcgcc gaagcacagg
      481 ccttcgacca aagtgatcgt ttccatggac cctccagagg tggccgctta gcttagcacg
      541 ccgacaggta caggttggtt ggccagacct ttttctgtag tcttgcgaat acctcgaccg
      601 ctggggaatg acggtggcaa taaggcctgc gtctgctcag cctgcagggt ctggccctgg
      661 gtcgaaagca acgaggaggg atagatgagt acgatcaaga actggatgat ggcgctgctc
      721 ggcgttgtcg ttctcgtcgg gtttgtcggc tgcggccagg acgcagggtc cggacccgct
      781 gatgcgcccg ccgcggcgga agaggcagcc gcggatgaga ccgcgggtgg gttcattcag
      841 cctgccgctg aagcagccga agaggctatg gacgagggga tggaagcgct tgaggaagcc
      901 gctgacgaaa ccgccaccga agctgcagca gaggcagccg agatgatgga agaagcagcc
      961 gcggaggcag aagaggcagc cgaatcggcc atgggggaga tgaacatcac cccgccgggc
     1021 tccaactagc cgccttccac ttccagccaa cgggtcagtg cagtttcgaa ctgttcgaag
     1081 tctggcccgc tcgtgatcag gtaacggtcc gaacgcggcg cggtgcgcca gcctcgggcc
     1141 gtttctgttt gccctgcagc gaagcggtca gcgtcattgg cgcgcgtatg acgagggtgc
     1201 gggcccagcc agccgagacc gtgtcttttt ttttctggta cgctcccggg gcacttttgc
     1261 cggggcgctg caaaacccag ggcctgcgtt gagcgaacaa cttgaaaggg gaacatcatg
     1321 aaagacgtgg tcatcgtcga tagcgttcga accgggctcg ccaaatcctt ccgtgggagc
     1381 ttcaacctca cccgctccga cgacatgctc gcgcactgca tcgacgcgct gctcgacagg
     1441 aacggcggcg tatcgccgca cgaggtggaa gacgtcgtgg tgggggcggc tagccacgtc
     1501 ggcgagcagg acggcaacct ggcccgactg gccgtggtgc tgtcgaagct gccgatcacg
     1561 accgccggcc tgaccatcaa tcgcttctgc tcgtccggcc tgcagtccat cgccatcgcc
     1621 gccaaccaga ttgcgaccgg ccagactcag gtcgccattg caggcggcgt cgactcgatc
     1681 tccatgcgta cccgtcagga ggcggccaag gccgagctca acaagcggct ggtggaggag
     1741 aaaccggaca tctacatggc catggggaat accgccgagg tggtcgcgcg gcgctatcag
     1801 gtgacccgcg aagcccagga cgagtatgcc ctgcagagcc agcaaagata cgcggccgcc
     1861 gctgaggccg gatatgtcgc cgaagagatc gtgcccatgg cggccaccat gctcaaggtg
     1921 gacaaggaga cgggtgaaga aagccgcgtg gacgtcgtcg tcgaccggga cgagtgcaac
     1981 cgccccacca ccaccctgga aggtctggca tcgctgccgc cggccttcga ggaagatggc
     2041 tccgtcactg cgggaaatgc ttctcagctc tccgacggcg cgtccatgac gctgctcatg
     2101 agcgctgagc gcgcagcgca gctgggccta gaaccgttgg gcatctaccg gggcttcacc
     2161 gtggccggct gcgagcccga cgagatgggt atcggcccgg tgttcgccat cccgcggctg
     2221 ctggaaaacg cgggtctcaa gcaggatgac gtcgatctgt gggagctcaa cgaggcgttc
     2281 gcgtcgcagt gcctgtattg tcgggatgcg ctgggcatcg acaacgagat ttacaacgtc
     2341 aacggcggca gcatcagcat cggccatccg ttcgggatga ccggctcgcg catgacgggc
     2401 ctggttctcc gcgagctgaa aaggcgtggc aagaggtacg gagtcgtcac catgtgcatt
     2461 ggcggtggcc agggtgcagc aggtctgttc gaggcagcct gatcagcccc cggggttaag
     2521 taacaaattg aaacccaagg gaaatggcgc tgaaacagcg cagagtcaga attgccgtcg
     2581 ctctggggat aattcgcgag gcctgtgcgc gttcttgtag gacacgtaga ggctgccggc
     2641 accataagca tttatagtgg tccaatcatc acggggcgga aaatgggagc atccggtggg
     2701 cggatgcggt cggagctccg aatggaacag gggttacgtt catcagaagc ctgaccctga
     2761 gcgttttgct cttcacgtca ctgtccggcg gcgctttcgc cgccgacgag gctgtgcgtg
     2821 cgggcgatgc ggccagcatc ccgaacgt
//
LOCUS       DBNHKV010000164        14840 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_164344, whole genome shotgun sequence.
ACCESSION   DBNHKV010000164 DBNHKV010000000
VERSION     DBNHKV010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 14840)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14840)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14840
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_164344"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="AC16PP_05250"
     CDS             <1..1062
                     /locus_tag="AC16PP_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR6481067.1"
                     /translation="ELRYWGYSIHELAEYSTFEETAYLLLHGELPTAAELAAFDGELK
                     AARRLPDGLVDIIRDVQQAHPMDVLRTAVSALAAFEPDVADTSRDGVLRKGIRLAAQV
                     PAIVAAHHRLRSGLAPVEPDDDLGHAAHFLYGLKGEVPSENAARLMDLDLILHAEHGS
                     NASSFTARVVTGTDANLHAAITAAIAALSGPAHGGAAEDVMRMAREIGEPQNAAAYVK
                     EKRGRREPIMGFGHRVYRAEDPRARHLRSGVERLSEEMGEPHWNEILKAVVEAMQPYA
                     RLGVNVNVDFYSGVIYHLHGIPQDIFVPIFAVGRVPGWTLQVLQQMESNILIRPLLEY
                     NGPELRSYVPMDERSAAGS"
     gene            1059..2030
                     /locus_tag="AC16PP_05255"
     CDS             1059..2030
                     /locus_tag="AC16PP_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HIR6481068.1"
                     /translation="MSFVETFLERARSLGRSVVFPEGQDPRILEAARCLADDGVAGIV
                     LLGSRDRIEAAAAEFQISLGGFRLIDPASGSVPQDYVDGYLANRPGARTGMAERAMRK
                     PLFFGAMMVKAGDADAMVAGAASPTRRVIEAARLCIGLGEGISGPSSFFLVRVPSRDQ
                     PFVFADCAVNVDPDAEELAAIALASGASAGQLLDAPPRVALLSFSTRGSATHPRVDKV
                     RHALEIVRACRPSFPVDGEFQADAALVPQVAVSKVGGDSEVAGRANVLIFPDLDAGNI
                     AYKLTQHLAGAEVVGPILQGFARPVADLSRGAGVDEIVATTALTLCL"
     gene            2048..3214
                     /locus_tag="AC16PP_05260"
     CDS             2048..3214
                     /locus_tag="AC16PP_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HIR6481069.1"
                     /translation="MRIVEARELTVPLEGEVANAVVSFAGHTVSLVALISDQRRNGRP
                     VVGFGFNSIGRFAQGGILRERVFPRLLQAEPDTLLDPSGAAFDPAAVAAAVMRNEKPG
                     GHGDRAGAVAAVELAVWDLNAKLADEPAWATIARHFGRETQRKGAPVYAAGGYYYPED
                     SLERLRLELDSYRALGFDSFKIKIGGAPMDADMARIEAALVVAGEGSRLAVDANGRFG
                     LADALGYARALEPLRLRWFEEAGDPLDYALNADLTGAYSGPVATGENLFSGSDAVNLA
                     RFGGMRPGKDVFQMDPGLSYGLTEYAGMLAALEAQGFDRAQCWPHGGHMINLHVVVGL
                     GLGGCEAYPGVFQPFGGYAPECRIDGGRVWPSQAAGFGLEQKRELGPSLGALSA"
     gene            3255..4187
                     /locus_tag="AC16PP_05265"
     CDS             3255..4187
                     /locus_tag="AC16PP_05265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR6481070.1"
                     /translation="MHIAIVGCGAMGSVYAALLASAGHRVLAVDTDENHVAAINAGGL
                     RVSGASGDRTVAIDAYTTAPDVRVDLVVVAVKAAHVGAAAPAVSRLVGPDTLVLTIQN
                     GLGSLTELADAVGSEKLLVGVAQGFGASLPQPGHAHHNDMKAIRMGAYGAASNEAVEK
                     VAAGWRAAGFDAAAVADVAAVQWEKLICNVAYSAPCALTGMTVGEVMDDPDVGPISRA
                     AATEAFEVARALGIALGFEDPVAHVREFAGRMPLAKPSVLLDVEAGRPSEVGVINGAV
                     VREAARAGVPAPVNDTLTRLVLALERHGRARSRA"
     gene            4232..4597
                     /locus_tag="AC16PP_05270"
     CDS             4232..4597
                     /locus_tag="AC16PP_05270"
                     /inference="COORDINATES: protein motif:HMM:NF025887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6481071.1"
                     /translation="MDDMQELLAADARRRAAMIAGDVDGLSRLLDDDLVWTHSSGRTD
                     DKTAFLGAIAEGTVTYLSLETEDVSVSRHGEVYLCHGILNGRASRDGAEKALRNKFLS
                     VWRRANGAFAMLAWQSTGF"
     gene            4702..5454
                     /locus_tag="AC16PP_05275"
     CDS             4702..5454
                     /locus_tag="AC16PP_05275"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase-like domain-containing
                     protein"
                     /protein_id="HIR6481072.1"
                     /translation="MHRLLASCAEPQEEISPASPPPTIYAAGTLITMDPGLPRAEAVA
                     VADERILAVGTLAELRGRYPGAAVDETFAERVVMAGLIDQHLHPFLAALTMTAEVVSI
                     EEWVLPARMFPAARDRDAYLCLLGAAAANMEDPAEPLISWGYHHYLHGSLTRKDLDAI
                     SESRPIMVWHRSAHEFVLNTPALAAAGVTEALVAAEEEVIREQIDLADGHFWERGSFD
                     FLVPRLMPMLAAPDRFRAGLAALTGGCEIRWA"
     gene            5489..6961
                     /locus_tag="AC16PP_05280"
     CDS             5489..6961
                     /locus_tag="AC16PP_05280"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006720 - isoprenoid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6481073.1"
                     /translation="MRVELIESFASGLPEEDDHPYRTGAWQPNVREYDAVDLTVTGRL
                     PDDLSGVYLRNTENPLHDAIGRYHPFDGDGMLHALFVDNGEAEYRNRFIRTDGFAAEL
                     AEGRPLWAGLMESPKKSLREGCGARTRLKDASSTDVVVHGGRAVTSFYQCGDLYEFDP
                     RSLTQLGKADWVAGTTPGWGISAHTKVDEATGEMLFFNYAKEAPFMHYGVVNAARELV
                     HYVPVPLPGPRLPHDMAFTDNYAILNDFPLYWDPQALRAGAHAVRFFADQPSRFGIIP
                     RRGGSHEVRWFEAKPTFVLHWINAYEDGDEVVLDGFFEENPNPKLTIDPNDKRSMFRM
                     LDMHVIGAKPHRWRFNLRTGATREEYLDDAVSEFGMINPAHGGKPYRYVWAVTLKPGW
                     FLMDGLVRLDVQTGEKQVYQLPDGVFLSESPMAPRVGSTAEDDGYVVTFVTDMNRDAS
                     SAMIFDAADIEQGPVAQVQLPERICVGTHSCWSDKRNFAR"
     gene            6981..7904
                     /locus_tag="AC16PP_05285"
     CDS             6981..7904
                     /locus_tag="AC16PP_05285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6481074.1"
                     /translation="MKIGLATPDGLWASDLDSRRELLAQVAAAGVDHVFMADHVSFRN
                     GSGTDGFVEIAGLSQLHPTLGVMISIYLLPLRHPVPVARQLATVNRLAPGRVLFGVGI
                     GGEDRHEIEVCGVDPATRGRRMNESLSVLRGLIGGEEVTFHGEFFDLDRALIRPAIEP
                     AIPIIVGGRSDAALVRAGRLGDGWIGAWCSVRRFSQALGIIDDAAEQAGRVAVDWQHG
                     YQPWVGVGDTRDEARGRVAEAMEAFYKVPFESFEKYTPFGTPDEVASQLGGYVEAGCR
                     LMNFKVVAGNDAEAIAAMGEIGRALREVAAA"
     gene            complement(7901..9094)
                     /locus_tag="AC16PP_05290"
     CDS             complement(7901..9094)
                     /locus_tag="AC16PP_05290"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR6481075.1"
                     /translation="MSGPRTFSKSFLQQEPLPDAAIEAAVAVMGSGRLHRYDAMADDP
                     GETALLEMEFARYQGSRYALACASCGYALYVAMLSAGVGPGDGVLINGFTLAPVPGAV
                     TNCGAQAIFVDTADDLTVDLNDLERKAADASARFLLLSHMRGHIADMDAVTGICEENG
                     LTLIEDCAHTLGARWKGRLSGSFGAVSCFSTQTYKHMNSGEGGLLVTNRDDIMARAIL
                     YSGSYMLYDRHLAGPPPEAFEGLSLTTPNYSGRMDNLRAATLRPQLARLDESCRRWNE
                     RYDVLAEGLAALPGVRLPERSPAESFVGSSIQFQLPGRTPAEMEDIVDRCAARGVALK
                     WFGADEPRGYTSRFDSWRYLGEPPELPGTRNALATLLDMRVPLTFDLEDCRHIVDIIA
                     DVIAG"
     gene            complement(9091..10425)
                     /gene="hisD"
                     /locus_tag="AC16PP_05295"
     CDS             complement(9091..10425)
                     /gene="hisD"
                     /locus_tag="AC16PP_05295"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR6481076.1"
                     /translation="MATTYLKRAEPAAAPDEEGIAVRVAEMLAKIRSEGEPAVAGYAR
                     DLDGFDGDFVLSREARDRLIAQLPSETRDDITFAWRQIKRFAEAQRASLHEFEIQTEP
                     GVTLGQRIVPVQVAGCYVPGGRYAHAASALMSVTTAKVAGVPTVVACSPPRGGAIHPA
                     VAFAMDLAGADVIVQMGGVQAVATLAFGLFGIPPADLLAGPGNAYVAAAKRQLFGEVG
                     IDVIAGPTESAIIADGGADPRLIAVDLASQAEHGPDSPVWLITSSREVAERVMALLPV
                     VAAALPNQDVVMTAWRRHGEVVLCEGREEMVRVSDAYAPEHLQILADDHGWWLEHLSN
                     YGSLFLGEGSTVTHGDKCSGTNHILPTRGAARYTGGLNVQKFLKVLTWQKLDEQANLS
                     FSAVGSRISRVEGMEGHARACDWRLRRYFPGQDWPFEVHGFDELTPGTDEPA"
     gene            complement(10425..10703)
                     /locus_tag="AC16PP_05300"
     CDS             complement(10425..10703)
                     /locus_tag="AC16PP_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481077.1"
                     /translation="MANGVIRIPALWLIASAVQAAEDFTLELSGGRVMDADITVFDPL
                     TVEDQATYQNPYQPSAGIRHVIVNGVFVVRDEQFVENTFPGRRISSSL"
     gene            10764..11285
                     /gene="thpR"
                     /locus_tag="AC16PP_05305"
     CDS             10764..11285
                     /gene="thpR"
                     /locus_tag="AC16PP_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HIR6481078.1"
                     /translation="MRCFVACFLSAESAHRLGTRLEAAWTPPARARLVPRANYHVTLR
                     FFGEVCSAEVSGLLAAVAELGGHDLSCTTGPVTGLPRPARARVIAVGVEPSSPLTDWA
                     EALSGIDPEPEGRAFLPHVTLARIRGTMRVPQIGGLAGVELALHAPSLYETVPVDGGV
                     RYRRVEPERFGSG"
     gene            complement(11277..12296)
                     /gene="mtnA"
                     /locus_tag="AC16PP_05310"
     CDS             complement(11277..12296)
                     /gene="mtnA"
                     /locus_tag="AC16PP_05310"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIR6481079.1"
                     /translation="MNDPGFPPSAISWQDGRLRLLDQRRLPEEVAYLEIRTVDAAHEA
                     IRTLAVRGAPAIGIAAAYTLVVAMVADAPTDAAGFRARLAEHARHLTSARPTAVNLAW
                     ACARLLSAAERSADLTTIQAEAEAIHEEDRLICRAIGSHGQPLIPDNAGVLTHCNAGA
                     LAVSELGTATAPMYLSHAAGKPFRVFVDETRPLLQGARLTAWELAAAGMDVTLICDSA
                     AAAFMAAGEIDLAIVGTDRVTANGDVVNKIGTLGVAVLCRHFGIPFYVALPSSTFDPH
                     TATGAQVPIEERDPAEVLGDRAADVPARNPAFDVTPANLVTAFITDRGVLRPPFAESL
                     SSLSA"
     gene            complement(12298..12927)
                     /locus_tag="AC16PP_05315"
     CDS             complement(12298..12927)
                     /locus_tag="AC16PP_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012804.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR6481080.1"
                     /translation="MAEQEGVIRFAYDLQSPDGVVTEESLRETLAAWRTIFRRLGVLG
                     HDPDRYGGLGFGNLSVRDREHPEEFVVTASQTSGRSGLRDRDLVRITGCNLERFWVDA
                     VGHKPPSSETLTHAMIYAADPRIQWVFHVHSPEIWRHAEQLAIPCTAPEVAYGSADMV
                     RAVAELLEKHQSRPLLFATLGHEDGIFACGPTARDAGGLLVSYLAKALA"
     gene            13010..13861
                     /locus_tag="AC16PP_05320"
     CDS             13010..13861
                     /locus_tag="AC16PP_05320"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6481081.1"
                     /translation="MSGGSRRSWRAMTGALGAIAPPGDALDLPAAEIVQALNAHQVVA
                     FRNQSLDPAELVAFASRLGELDVYPLAEPLPESPFVVAVLKNPEDTSNFGGAWHTDTS
                     YVREPPKFTVLYAVEIPGSGGDTLFADMYQAFAGLSPGLQRCLERVQAVYTADLVHAG
                     SGEYTAVAGQSVPAKESERATTVEHPVVRVHPETGRKALYTSLIHTERFVGMTREESL
                     PLLEYLQSVSVAAENCTRLHWQAGTLAIWDNRCVQHYPLNDYPGERRLMHRVIVRGEE
                     PVGVEPL"
     gene            13945..14475
                     /locus_tag="AC16PP_05325"
     CDS             13945..14475
                     /locus_tag="AC16PP_05325"
                     /inference="COORDINATES: protein motif:HMM:NF049802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:14230..14232,aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HIR6481082.1"
                     /translation="MVDYHDPRGEVATPVDAYLLSVDLESSNDPRVAFLANGFPDSDV
                     FLEQLAEVMSEKLTSLRAEHYNKGNASIAAPQDMLEEIREGCVAAVAAYGHUGSCTTG
                     TVRDGINMARLGIPAVALVTEDFWPQGDFIAGSLGMPDIPRVRLPHPVAGTGVANLRK
                     IAQEIAPEVFAALSRN"
     gene            14485..>14840
                     /locus_tag="AC16PP_05330"
     CDS             14485..>14840
                     /locus_tag="AC16PP_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481083.1"
                     /translation="MSGLFTARSEAAAVEHLHELGCTDGLPVIVPTPDRVERMVLASG
                     QDPAAVLGEMGPGKGIATIEKVAVAAVMAGCLPDYMPLVVAAVRAVIDPVFDLTEMQG
                     TTHCTAPLIIVNGPAR"
BASE COUNT         2319 a         4869 c         5181 g         2471 t
ORIGIN      
        1 gagctgcgct actggggtta ctccatacac gagctcgccg agtactcgac gttcgaggag
       61 acggcctacc tgctgctcca cggggagttg cccaccgccg ccgagctggc cgccttcgat
      121 ggggagctca aggcggcgcg acggcttcct gatggtctcg tcgacatcat ccgcgacgtg
      181 cagcaggcac accccatgga cgtgctccgc actgccgtgt cggcgctggc agcattcgaa
      241 ccggacgtgg cagacacatc gagggacggc gtcctgcgca aaggcattcg gctcgccgcc
      301 caggttccgg caatcgtggc cgcgcaccat cgcctccgat cgggcttggc acccgtggag
      361 cccgacgacg atcttggcca tgcggctcat tttctctacg ggctgaaggg cgaggtgccc
      421 agcgaaaacg cggcccggct gatggatctg gatctcatcc tgcacgccga gcatggctcc
      481 aacgcctcgt cgtttaccgc acgagtggtc accggcaccg acgccaacct ccacgcggcg
      541 atcacggctg ccatcgcggc gctctccggt cccgcccacg gaggcgccgc cgaggacgtc
      601 atgcgcatgg cccgggaaat cggcgagccc cagaacgccg cggcctacgt caaggagaag
      661 cgcggacgcc gggagcccat catggggttc gggcatcgcg tctaccgcgc cgaggatccc
      721 cgggcgcgac acttgagatc cggcgtggag cggctatccg aggagatggg cgagccgcac
      781 tggaacgaaa ttttgaaggc ggtggtcgag gccatgcagc cttatgcccg gctgggcgtc
      841 aacgtgaacg tcgacttcta ctccggggtg atctaccacc tccacggcat cccccaggac
      901 atcttcgtgc ccatcttcgc cgtcggccgg gtgccgggct ggacgctgca ggtgctccag
      961 cagatggaga gcaacatcct catacgccct ctgctcgagt acaacggacc ggagctgcgc
     1021 tcttacgtgc ctatggacga acgctccgcg gcggggtcgt gagcttcgtc gagacctttc
     1081 tcgagcgcgc caggtccctg ggccggagcg tcgtctttcc agaggggcaa gacccccgca
     1141 tcctcgaagc ggcgcgttgc ctggcagatg acggggtggc cgggatcgtg ctgctcggca
     1201 gccgcgatcg catcgaggcc gcggccgcgg agtttcagat ctcgttgggc gggttccgac
     1261 tcatcgatcc cgcgtccggg agcgtgcccc aggattacgt ggacggttat ctggccaacc
     1321 gaccgggcgc tcgcaccggc atggcggagc gggcgatgcg caagccgttg ttcttcgggg
     1381 ccatgatggt caaggcgggg gatgcggacg ccatggtcgc gggagccgcc tcgccgactc
     1441 gccgcgtcat cgaggccgcc cgtctctgca tcgggctcgg agaggggatc tcggggccgt
     1501 ccagcttttt cctggttcgc gttccgtcca gggatcagcc cttcgtgttc gccgactgtg
     1561 ccgtcaacgt cgacccggac gccgaggagc tggccgccat cgcccttgcc tcgggcgcga
     1621 gcgcggggca gctgctggac gcgccgccgc gggtggctct gctgtcgttt tcaactcgcg
     1681 gcagcgcgac ccatccccgc gttgacaagg tgcgccacgc gctggagatc gtccgcgcct
     1741 gccggccgtc attccccgtg gatggtgagt ttcaggcgga tgccgcattg gtgccccagg
     1801 tggcggtgag caaggtcggc ggcgacagcg aggtggccgg gcgtgccaac gtcttgatct
     1861 tcccggacct cgatgcgggc aacatcgcct acaagctcac ccagcatctg gcaggggcgg
     1921 aagtggtggg acccatcctg cagggctttg ccaggcccgt cgccgatctg tcgcggggtg
     1981 cgggcgtcga cgagatcgtc gccaccaccg cgctgaccct ttgcctttag cgatcgtctg
     2041 ctgagtcgtg cgcatcgtcg aggcaagaga gctcacggtt cccctggagg gggaggtggc
     2101 caacgcggtg gtcagcttcg ccgggcacac ggtttcgctc gtggcgctca tcagcgacca
     2161 gcgacgcaac ggacggcccg tcgtcggctt tggcttcaac tccatcgggc ggttcgctca
     2221 gggcggcata cttcgggagc gcgtctttcc ccgcctgctg caagccgaac cggacaccct
     2281 gctggacccg agcggtgcgg ccttcgaccc ggccgcggtg gcagcagccg tgatgcgcaa
     2341 cgaaaagccc ggcgggcacg gtgaccgggc gggtgcggtg gctgcggtgg agctcgcggt
     2401 atgggacctc aacgccaagc ttgccgacga gcccgcctgg gcgaccatcg cccggcattt
     2461 cggccgggaa acgcagcgta agggcgcgcc cgtctacgct gcgggcggct actactaccc
     2521 cgaggactcc ctggaacgcc ttcgcttgga actggattcc taccgcgccc tcgggttcga
     2581 ttcgttcaag atcaagatcg gcggcgcgcc catggacgcc gacatggcca ggatcgaggc
     2641 ggcgctggtg gtcgcgggtg agggctcgcg gctcgccgtg gacgccaatg ggcggttcgg
     2701 gctggcagac gcgctgggct atgccagggc gttggagccc ctccggctgc gctggttcga
     2761 ggaggcgggc gatccgctgg attacgcgct gaacgcggac ctcacaggcg cctattccgg
     2821 ccccgtggct accggcgaga acctgttcag cgggtccgat gcggtcaacc ttgcgcggtt
     2881 cggcggcatg cgccccggta aggacgtctt tcagatggat ccgggcctga gctacgggct
     2941 cacggagtac gcgggcatgc tggcggcgct ggaagcccag ggtttcgatc gggcccagtg
     3001 ctggcctcat ggcgggcaca tgatcaatct gcacgtcgtg gtggggctcg gcctcggcgg
     3061 ctgtgaagcc tacccgggcg tctttcagcc tttcggcggc tacgcgcccg agtgcaggat
     3121 cgacggtggc cgggtctggc cctcccaagc ggcgggattc ggtttggagc agaagcggga
     3181 gctcggccct tctctcgggg cgctctcagc gtgagcggca ggtctttcgg ggcagcattc
     3241 agggaggtga aggcgtgcac atagccatcg tgggctgcgg ggccatgggg tcggtctacg
     3301 cggccctgct cgccagcgcg ggtcatcgcg tgcttgccgt ggatacggac gagaaccatg
     3361 tggccgccat caatgccggc ggcttgcgcg tctccggtgc cagcggcgac cgcacggtcg
     3421 ccatcgatgc gtacacgacc gcgcccgacg tgagggtgga cctcgtggtg gttgcggtaa
     3481 aagcggccca cgtcggtgcg gcggcgcccg ccgtctcgcg cctggttggc cctgacactc
     3541 tggttctgac cattcagaac ggtctcggca gcctgaccga gctcgccgac gcggtaggat
     3601 cggagaagtt gctcgtcggc gttgcccagg ggtttggcgc gtcgctgccc cagcccggcc
     3661 acgcccatca caacgacatg aaggccatcc gcatgggcgc gtacggcgcg gcctcgaacg
     3721 aggctgtaga aaaggtagca gccgggtggc gcgccgccgg cttcgacgcc gcggcggtgg
     3781 ccgacgttgc cgccgtgcag tgggagaagc tcatctgcaa cgtggcctac agcgcgccct
     3841 gcgcgctgac ggggatgacg gtaggggagg tcatggacga tccggacgtc ggaccgatca
     3901 gccgcgccgc ggccacggaa gccttcgagg tggcccgggc gctcggcatc gcccttggct
     3961 tcgaggatcc ggttgcccac gtccgcgagt ttgccgggcg aatgccgctg gcgaagccct
     4021 cggtgctcct ggacgtcgag gccgggcgcc cgagcgaggt gggggtgatc aacggtgcgg
     4081 tggtgcggga ggcggcgcgc gcgggggttc cggcccccgt caacgatacg ctcaccaggc
     4141 tcgttctggc cctcgaacgg cacgggcgcg cacgcagccg ggcgtgagct gaccgaggag
     4201 gttggaacga cgatgaatga ggaagcaggc aatggatgac atgcaggagc tgctggcagc
     4261 ggacgcccgg cgtcgcgctg cgatgatcgc cggcgacgtg gacgggcttt cccgtcttct
     4321 cgacgatgat ctggtctgga cccactcttc cggcaggacc gatgacaaaa cggcctttct
     4381 gggcgccatc gccgagggga cggtcactta cctttccctg gaaacggagg acgtcagcgt
     4441 ctcccgtcac ggcgaggtct atctctgcca cggcatcctc aacggccggg caagcaggga
     4501 tggcgcggag aaggcgctgc gcaacaagtt cttgagcgtg tggcgtcggg cgaacggcgc
     4561 cttcgccatg ctggcctggc agtccacggg gttctgatgc gcggtggcgg cgcgccacgg
     4621 cgcggcgtcg cactggacaa gcgccgacgg ctccggctta tgctcgcgcc atgaggatcg
     4681 acgagaccat caacgaggcg tttgcaccga ttgctcgcgt cctgcgcgga gccgcaggaa
     4741 gaaatctcgc ccgcgagccc gccgccgacg atctacgcgg cgggcacgct gatcaccatg
     4801 gaccctgggc tgccgcgggc cgaagcggtg gccgttgccg atgagcgcat cctcgccgtg
     4861 gggacgctgg cggagctgcg cggccgctat cccggggccg cggtagatga aacctttgcc
     4921 gagcgcgtgg tcatggccgg cctgatagat cagcacctgc acccgtttct ggcggcgttg
     4981 accatgacgg cggaggtggt ctccatcgag gaatgggttc tcccggcccg gatgtttcct
     5041 gcggcccggg atcgtgatgc gtacctttgc ctgctgggcg ccgcggcggc gaacatggag
     5101 gatccggccg agccgctgat ctcgtgggga taccaccact acctccacgg aagcctcacc
     5161 cgaaaggacc tcgacgccat cagcgagtcg cggcccatca tggtgtggca ccgttcggcc
     5221 cacgagttcg tcctgaacac gccggccctg gctgcagcgg gggtgaccga ggcgctggtc
     5281 gcggccgagg aggaggtgat tcgcgagcag atcgacctgg cggacgggca tttctgggag
     5341 cggggcagtt tcgattttct ggtgccccga ctgatgccca tgctcgccgc cccggatcgc
     5401 ttccgcgccg ggctcgcggc gctgacgggc ggatgtgaga tacgatgggc ttgaatatgt
     5461 gcatggtgag acaaaggggg agcggcagat gagagtggag ctgatcgaat ccttcgcctc
     5521 gggccttccc gaggaggacg atcatcccta ccggacgggt gcctggcagc cgaacgttcg
     5581 cgagtacgac gccgtggacc tgacggtcac cggccggctc cccgatgact tgtcgggcgt
     5641 gtacctgcgc aacacggaga acccgctgca tgacgccatc gggcgctacc acccctttga
     5701 cggtgacggc atgctgcacg cgctcttcgt ggacaacggg gaggccgaat atcgcaaccg
     5761 gttcatccgc accgacgggt tcgcagccga gctggccgaa gggcggccct tgtgggccgg
     5821 gctcatggaa tcgcccaaga agtcgctgcg cgaaggctgc ggggcgcgca cgcgcctcaa
     5881 ggatgcttcc agcacggatg tggtcgtcca cggcggccgg gcggtcacca gcttctacca
     5941 gtgcggcgac ctgtacgagt tcgatccccg ctccctcacc cagcttggca aggccgactg
     6001 ggtggccggc accacgcctg gctggggcat atcggcccac accaaggtgg acgaggccac
     6061 gggcgagatg ctctttttca actacgccaa ggaagctccg ttcatgcatt acggcgtggt
     6121 caacgccgcc cgcgagctgg tgcactacgt tccggttccg ttgcccggcc cgcgcctgcc
     6181 acacgacatg gcgttcaccg acaactacgc gatcctcaac gacttccctc tctactggga
     6241 cccgcaggcg ctgcgagcgg gcgctcacgc cgtgcgattc tttgccgatc agccgagccg
     6301 cttcggcatc atcccgcgcc gcggtggttc ccacgaggtg cgctggttcg aggcgaagcc
     6361 cacgttcgtg ctgcattgga tcaacgccta cgaggacggc gacgaggtgg tcctcgacgg
     6421 cttcttcgag gagaacccga atcccaagct gaccatcgac ccgaacgaca agcgctcgat
     6481 gttccggatg ctggacatgc acgtgatcgg cgccaagccg catcgctggc gcttcaacct
     6541 gcgcaccggc gctacgcggg aggagtatct cgacgacgcg gtgtccgagt tcggcatgat
     6601 caacccggcc cacgggggta aaccctaccg ctacgtctgg gccgtcacgc tgaagccggg
     6661 ctggtttctc atggacggcc ttgtccggct ggacgttcag accggcgaga aacaggtcta
     6721 ccagctgccg gacggcgttt ttctcagcga aagccccatg gccccgcggg ttgggtcgac
     6781 ggccgaggac gacggctacg tggtgacctt tgtcacggac atgaacagag acgcgtccag
     6841 cgccatgatc ttcgatgccg ccgacatcga gcagggtccc gtcgcccagg tccagctgcc
     6901 cgagcgcatc tgcgtgggca cccactcgtg ctggtccgac aaacgcaact tcgcgcgctg
     6961 aacgggcggc cggaaccatc gtgaagatcg gtcttgccac gcctgacggc ctatgggcgt
     7021 ccgacctcga cagccgccgg gagctgctgg cgcaggtcgc ggccgccggg gtcgaccacg
     7081 tcttcatggc cgaccacgtg tctttccgca acggctcggg caccgacggc ttcgtggaga
     7141 tcgcgggcct ttcgcagctc catccgacgc tgggcgtgat gatcagcatt tacctgctgc
     7201 ccctgcggca tcccgttccc gttgcgcgcc agctcgccac cgtgaatcgt ctggcgccgg
     7261 gccgggtact cttcggggtc ggcatcgggg gtgaagacag gcatgagatc gaggtttgcg
     7321 gcgtcgatcc tgccacacgg gggcggcgca tgaacgaaag cctcagcgtg ctgcgcggcc
     7381 tcattggtgg cgaggaggtg acctttcatg gcgagttctt cgatctcgac cgggcgctga
     7441 ttcggcccgc catcgagccg gcgattccca tcatcgtggg cggccgctcc gatgccgctc
     7501 tcgtccgggc cggtcgcctc ggcgatggct ggatcggtgc gtggtgctcg gtgagacgct
     7561 tctcccaggc gctcgggatc atcgacgacg cggctgagca ggccggacgg gtcgccgtgg
     7621 actggcaaca cggctaccag ccctgggtgg gcgtggggga cacccgcgat gaggcgcgcg
     7681 gccgagtggc cgaggccatg gaagcgttct ataaggtgcc gttcgaaagc ttcgagaagt
     7741 acacgccctt cggcaccccc gacgaggtcg cctcgcagct gggaggttac gtggaagccg
     7801 gatgccggct catgaacttc aaggtggtgg ctggcaacga cgcggaagcc atcgccgcca
     7861 tgggagagat cggccgcgcc ctgcgtgagg tcgcggcagc ctagccggcg atgacgtcgg
     7921 cgattatgtc gacgatgtgc cggcaatcct ccaggtcgaa ggtgagcggc acgcgcatat
     7981 ccagcagcgt ggccagggcg ttgcgggttc caggcagctc tggcggctcg ccgaggtagc
     8041 gccagctgtc gaatcggctg gtgtaccccc tgggctcgtc ggcgccgaac catttcagcg
     8101 caacgcccct ggcggcgcac cgatccacga tgtcctccat ctcggcaggg gttctgccgg
     8161 gcagctgaaa ctgtatcgag ctgccgacaa aggactccgc gggcgatcgc tcgggcagtc
     8221 ggacgccggg gagcgcggcg agcccttcgg cgagaacgtc gtagcgctcg ttccagcgcc
     8281 ggcagctctc gtcgagccgt gcaagctgcg gccgcagggt ggcggcgcgc agattgtcca
     8341 tgcggccgct gtagttgggc gtggtcagcg acaggccttc gaacgcctcc ggcggcggcc
     8401 cggcgagatg acggtcgtag agcatgtacg atccggagta gaggatggcc ctggccatga
     8461 tgtcgtcccg gttcgtcacc agcaggccgc cttccccgga gttcatgtgc ttgtaggtct
     8521 gggtgctgaa acagctcacg gccccgaagc tgccgctcag gcggcccttc cagcgcgcgc
     8581 ccagggtgtg cgcacagtcc tcgatgaggg tgagcccgtt ctcctcacag atccccgtca
     8641 ccgcatccat gtcggcgatg tggccgcgca tgtgggatag caggaggaag cgcgcactgg
     8701 catctgcggc ctttcgttcg aggtcgttca ggtccacggt cagatcatcc gcggtgtcca
     8761 cgaagatggc ttgggcgccg caattggtta ccgctcccgg gaccggggcc agggtgaagc
     8821 cgttgatcag cacaccatct cccgggccaa cgccggcgct gagcatcgcc acgtacaggg
     8881 cgtacccgca ggaggcgcag gccagagcgt agcggctgcc ctgataacgg gcgaactcca
     8941 tctccagcag cgcggtctcg ccgggatcgt cggccatggc gtcgtagcgg tgcaggcggc
     9001 cgctgcccat gacggccacg gccgcttcga tggcggcgtc cggcaggggt tcctgctgca
     9061 gaaacgactt gctgaacgtt ctcgggccgc tcacgccggt tcgtccgtgc cgggcgtgag
     9121 ctcgtcgaac ccgtgcacct cgaagggcca atcctgcccg ggaaaatagc ggcgcaggcg
     9181 ccagtcgcat gccctggcgt gtccctccat cccctccacg cgcgatatcc gcgagcccac
     9241 ggcgctgaac gacaggttcg cctgctcgtc gagcttctgc caggtcagca ccttcaggaa
     9301 cttctgcacg ttcagcccgc ccgtgtaacg cgctgcgccg cgagtgggga ggatgtggtt
     9361 ggttcccgag catttgtcgc cgtgagtgac cgtgctgccc tcgccaagga acagggaacc
     9421 gtagttgctg agatgctcca gccaccagcc gtggtcgtct gccaggatct ggagatgctc
     9481 cggcgcgtag gcgtcgctga cgcgaaccat ctcctcgcgg ccctcgcaca agaccacctc
     9541 gccgtgacgg cgccacgcgg tcatgaccac gtcctggttc ggcaaggcgg ccgccaccac
     9601 gggcaggaga gccatgacac gctctgccac ctcgcggctg gaggtgatca gccaaacggg
     9661 agagtccggc ccgtgctcgg cttgggaggc gagatcgacg gcgatcaagc gtgggtcggc
     9721 gcccccatcg gcgatgatgg cagactccgt gggaccggca atcacgtcga tgcccacctc
     9781 gccgaagagt tgccgctttg cggcggcaac gtaggcgttg ccgggcccgg cgagcaggtc
     9841 ggcgggaggg atcccgaaca ggccgaacgc cagcgtcgcc accgcttgga cgccgcccat
     9901 ctgcacgatg acgtcggccc cggcgagatc catggcgaag gcgacggcgg gatgaatcgc
     9961 gcccccccgc gggggcgagc aggcgaccac cgtcggcacg cctgccacct tcgcggtggt
    10021 tacgctcatc agcgcagacg ctgcgtgggc gtagcgccca ccgggcacgt agcatccggc
    10081 gacctgaacc gggacgatgc gctggccgag ggtgaccccg ggctcggtct ggatttcgaa
    10141 ctcgtgcagg ctcgcccgct gggcctcggc gaagcgcttg atctggcgcc aggcgaaggt
    10201 gatgtcgtcg cgggtttctg agggcagctg ggcgatcagc cgatcgcgcg cctcccggga
    10261 aaggacgaag tccccgtcga acccgtccag gtcccgggcg tatccggcaa cggcaggctc
    10321 cccctcactg cgtatcttcg ccagcatctc cgcgacgcgt accgcgatgc cctcctcgtc
    10381 cggcgccgcg gccggctcgg cccgcttgag ataggtcgtc gccatcacag gctgctgctg
    10441 atgcggcggc ccgggaacgt gttctccacg aactgctcgt cgcggaccac gaacacgccg
    10501 ttgacgatga cgtgtcggat gccagccgag ggctgatacg gattctgata ggtggcctgg
    10561 tcctcgacgg tgagcgggtc gaagacggta atgtccgcat ccatgacgcg gccgccgctc
    10621 agctccaacg tgaagtcctc ggcggcctgg accgcgctgg caatcagcca gagggccggt
    10681 atgcggataa ctccgttcgc catgccgcgt ctcccgcgcg ccgatcatga aggcgcaaga
    10741 tagaacggta gaatggggat gtcatgcgct gtttcgtagc ctgctttctg tctgccgaat
    10801 ccgctcaccg gctcgggacg cggctcgagg cggcgtggac accgcccgcc cgggcgcgtc
    10861 tggtcccgcg agcgaactat cacgtcacgc tgaggttctt cggggaggtc tgctctgccg
    10921 aggtatccgg gctgctggct gcggtggccg aactcggcgg tcatgacctt tcgtgcacga
    10981 cggggccggt gacgggtctt ccgcgtccgg cgcgtgcccg cgtcatcgcc gtgggcgtgg
    11041 agccctcgtc gccactgacg gattgggcgg aagcgctgtc gggtatcgat ccggaacccg
    11101 aaggccgggc gttcctgccc cacgtcaccc tggcccggat tcgcgggacc atgagggtgc
    11161 cgcagatcgg tggcctcgcg ggggtggagc tcgcgcttca cgcgccttcg ctgtacgaga
    11221 ccgttcccgt ggatggcggt gtgcgctacc ggcgcgtaga gccggagcgg tttgggtcag
    11281 gctgacaagc tgctcaacga ttccgcaaac ggcggtcgca atacgccgcg gtcggtgatg
    11341 aacgcggtca ccaaattcgc tggggtcacg tcgaaagcgg gattgcgggc aggcacgtcc
    11401 gcggcccgat cgcccagcac ctccgccgga tcgcgttcct cgatgggtac ctgcgcgccc
    11461 gtcgcggtgt gcggatcgaa cgtgctcgag ggcagcgcca cgtagaaggg aatgccgaag
    11521 tgccggcaga gcacggcgac gcccaaggtg ccgatcttgt tcaccacgtc gccgttggcg
    11581 gtgacgcggt ctgtccccac gatggccagg tcgatctccc ccgcggccat gaaggcggcg
    11641 gctgcgctgt cgcagatgag cgtgacgtcc atgcccgcgg cggcaagctc ccaggccgtc
    11701 agacgcgcgc cctgaagcag cggcctggtt tcgtcgacga acacccggaa cggcttgccg
    11761 gcggcatggc tgagatacat gggagccgtg gcggtgccca actcggaaac cgccagagca
    11821 ccagcgttgc agtgggtgag cactcccgcg ttgtcgggaa tcagaggctg gccgtggctg
    11881 ccgatggctc gacagatgag acggtcctcc tcgtggatcg cctccgcctc cgcctggatg
    11941 gttgtgagat cggccgagcg ctcggcggcg ctcagaagcc gagcgcatgc ccacgcgagg
    12001 tttaccgccg tggggcgtgc ggaggtgagg tggcgagcgt gttcggcgag ccgggcgcgg
    12061 aagccggcgg catccgtcgg cgcgtcggcg accatggcga ctaccaaggt gtaggcggcg
    12121 gcgatgccga tggcgggggc gcccctcacg gccagggtgc ggatggcttc gtgcgccgca
    12181 tccaccgtgc ggatctcgag ataggcgacc tcctcgggaa gccgtcgctg atcgagcagc
    12241 cgcaggcggc cgtcctgcca ggagattgcg ctcggtggaa acccagggtc gttcatgtca
    12301 ggccagcgct ttggccaggt agctcaccag cagcccgccc gcgtcgcgcg ccgtggggcc
    12361 gcaggcaaag atcccgtcct cgtggcccag ggtggcgaat agcagcgggc gtgactggtg
    12421 cttttcgagc agctcggcca cggcgcgaac catgtccgcc gagccgtagg ccacctcagg
    12481 cgccgtgcag gggattgcga gctgctcggc gtgccgccat atctccgggc tgtgaacgtg
    12541 gaagacccac tgaatccgag gatctgcggc gtagatcatg gcgtgggtga gcgtctcaga
    12601 cgacggcggc ttgtgcccca ccgcgtctac ccagaaacgc tccagattgc atccggtgat
    12661 ccgcaccagg tcccggtccc tgagcccgct gcggccgctg gtctggctgg cggtgacgac
    12721 gaattcctcc ggatgctccc gatcccggac gctcaggttt ccgaaaccca aaccgccgta
    12781 gcggtccgga tcgtggccga gaacgcccag gcgacggaag atggtccgcc acgccgcgag
    12841 ggtctctctc agcgactcct cggttacgac gccatcgggc gactgaagat cgtaggcaaa
    12901 gcggatgacg ccttcttgct cggccactcg tgtaatatcc ggcgatgttc ggtcacgcaa
    12961 agcatgccac gatgatgcgg gaaaaagcag cacatgccgg gagctctgcg tgagcggcgg
    13021 atcccggcgt agctggcgcg ccatgacggg cgcgctcggc gcgatcgcgc cgccagggga
    13081 tgcccttgat ctgccggccg ccgagatcgt ccaggcactc aacgcccatc aggtggtcgc
    13141 gttcaggaat cagtctctcg acccggcgga gctggtggcg ttcgcttccc ggctgggtga
    13201 gctcgacgtt tacccgctcg cagagcccct tcccgaatcg cctttcgtgg tcgccgtgct
    13261 caagaacccg gaggatacga gcaacttcgg cggggcatgg catacggaca cctcctacgt
    13321 ccgcgagccg cccaagttca ccgtgctcta cgccgtggaa attcccggca gtggcggtga
    13381 cacgctgttc gcggacatgt accaggcgtt tgccggcctg tcccccgggc tgcagaggtg
    13441 ccttgaacgc gtgcaggccg tgtatacggc cgacctggtg cacgcgggca gcggggagta
    13501 cacggcggtg gcggggcaga gcgttccggc gaaggagagc gagcgggcca ccacggtgga
    13561 gcacccggtg gtgcgcgttc atccggaaac cgggcgcaaa gcgctctaca ccagcctgat
    13621 ccacaccgag cggttcgtcg gcatgacgcg cgaggaaagc cttcccctgc tggagtacct
    13681 gcagagcgtt tcggtggccg ctgaaaactg cacccggctc cactggcagg ccggcacgct
    13741 ggccatttgg gacaatcgct gcgtgcagca ctatccgctg aacgactatc ccggagagcg
    13801 gcggctcatg caccgggtaa tcgtcagggg agaagagcct gtgggcgtgg aaccgctctg
    13861 aggcccattg ctgagctgga gttcgctgcc cattcccgtt ttaatcggtg tcatcgatca
    13921 gcaccctgca tcggaggtgg gtatatggtt gactatcacg acccccgggg agaggtcgca
    13981 acgccggtgg atgcctatct gctgagcgtc gatctcgagt ccagcaacga cccgcgtgtg
    14041 gcgttcctcg ccaacgggtt cccggattca gacgtttttc tcgagcagct cgccgaagtc
    14101 atgagcgaaa agctgacgag ccttcgcgcc gagcactaca acaagggcaa cgcgtccatc
    14161 gccgcgcccc aggacatgct cgaggaaatt cgcgagggct gcgtggcggc ggtggccgcc
    14221 tacggccact gaggcagttg cactaccggc accgtgcgtg acggcatcaa catggcaagg
    14281 ctcggcatcc cggcggtggc gctggtgacc gaggacttct ggcctcaggg cgacttcatc
    14341 gccggctcgc tcggcatgcc cgacattccc agggttcggc tgccccaccc ggtggcgggg
    14401 accggtgtcg ccaacctgcg caagattgcg caggagatag cgcctgaggt tttcgcggcg
    14461 ctgtcccgga actagggcga ggctttgagc ggattgttca cggcccggag cgaggcggcc
    14521 gcggtcgagc accttcacga gctcggctgc accgacggcc tgcccgtcat cgtgcccacg
    14581 ccggacaggg tggaacgcat ggtcctggcc tccggccagg atcccgccgc cgtcctcggt
    14641 gagatgggtc cgggcaaggg catcgccacc atcgagaagg tggcggtggc agcggtgatg
    14701 gccggctgcc tcccggacta catgccgttg gtggtggccg ccgtgcgggc ggtcatcgat
    14761 cccgtcttcg acctcacgga gatgcagggg accacccact gcacggcgcc cctgatcatc
    14821 gtcaacgggc ccgcgcggca
//
LOCUS       DBNHKV010000165        12766 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1935438, whole genome shotgun sequence.
ACCESSION   DBNHKV010000165 DBNHKV010000000
VERSION     DBNHKV010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12766)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12766)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12766
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1935438"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="AC16PP_05335"
     CDS             complement(<1..490)
                     /locus_tag="AC16PP_05335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6481084.1"
                     /translation="MGLLFQAVVFGIGFFCFTFWVAPWRAEFGSSRGEVMAILVTVQI
                     VMGCAAPFAGRAMDHLSIRALVVAGAVCFAAALALAAHATALWQIGLIYSVLFVAGAL
                     LAGPLAAQTLAAKWFFARRGTAIGLSSVGTSLGGFVLPLLVTWLIAAYGWRQANLVLA
                     ALV"
     gene            complement(578..1333)
                     /locus_tag="AC16PP_05340"
     CDS             complement(578..1333)
                     /locus_tag="AC16PP_05340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6481085.1"
                     /translation="MRLGALLGPIVDASEPNALADQARRYAGEGFESLWSAQAIGRGA
                     MYTDPFVALSVAAAVTEKVELGTAVVQVPLYHPVDLAHRALSLRQVAGERVLLGVGAG
                     STQTDFDAFGRDYASRFRTFGESVEVLRQLFEEGRAGETNLSPWPAVLGGLPLLLGSW
                     GKGVERAAASFDGWIASAHYRTPDQVVEALDGYRAAGGRRAVVSTILLGAGTDLGETG
                     DLLGRFAEAGFDDAVVMLLPGGPSATDVRRLVA"
     gene            complement(1364..2584)
                     /locus_tag="AC16PP_05345"
     CDS             complement(1364..2584)
                     /locus_tag="AC16PP_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6481086.1"
                     /translation="MNEPSGPLADITIIDCTMALAGPFGAAILADLGANVIKVEPPGG
                     DVARSVPPLPEDYANANSDQSAGCDFGGYFASVNRNKRSVVIDLKTPAGRETLLALCD
                     KADAIIENMRTGVMDRLGLGYEVLRARNPALVYGCIRGFGDPRTGRSPYAEWPAYDIV
                     AQSMGGIAHITGPESSHGFPAGASIGDLYPGTLLALGVVAAVHHARRTGEGQFMDVGM
                     YDAVMFLSETVIANFGYAGTELGPRGAHHPNLCPFGIFPTSDGAVAIAAPGPGHWQAL
                     CQAIGRPDLVDDEKTRNTFVRRKHQHFVEGVVSDWTATRTRAEVVAALGGRVPCGPVN
                     TAADIFADPHVAARGMVSQVELPGENGEVSIVGTPIKFTGTPTGLYRRPPMLGEHTEE
                     VLREMGLKGAANDE"
     gene            2722..3597
                     /locus_tag="AC16PP_05350"
     CDS             2722..3597
                     /locus_tag="AC16PP_05350"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6481087.1"
                     /translation="MGMLDGKVALVTGAGGGLGRAHALLLAQHGAAVVVNDLGGARDG
                     SGEGHAMADQVVAEIEAAGGRAAADYGSVSDPAAAKQMVQIAVDRFGKIDICINNAGI
                     LRDKSFKNMTDDMWDVVVDVHLRGTYLVAKAAWDQMIEQGTGGRIINTSSTSGLIGNF
                     GQANYGAAKAGIAGLTRVLALEGMKYGITVNTLAPAAWSRLTEDIMPEGMEERLAPEK
                     VSPAVVWLCTDDAANVTGRQFCAGQNRVSLLSWQVTEIADKNAMDTPWTVEEIGERMQ
                     ATMDAWPKLLKPMEV"
     gene            complement(3605..4399)
                     /locus_tag="AC16PP_05355"
     CDS             complement(3605..4399)
                     /locus_tag="AC16PP_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HIR6481088.1"
                     /translation="MIVEQIWTGNSYRNFNYLIVCPESGEALAIDPLDHKKCLARARD
                     KGWQITQVLNTHEHPDHTGGNGAVIEATGAKLLAHANAQRSIKGMDVALNAGDVVKVG
                     RTVELLALDTPGHTMAHVCLLSQTETPALFCGDTLFNAGAGNCHGGGHPDELYQTFAS
                     QLDQLPETTLVYPGHDYITNNLAFTLDREPDNEAAAALLKELEANHDPDAALVTSMAQ
                     EKKINTFFRLTSRTVIDRLREAFPDLPDEPTPHEVFVKLRELRNSW"
     gene            complement(4454..5248)
                     /locus_tag="AC16PP_05360"
     CDS             complement(4454..5248)
                     /locus_tag="AC16PP_05360"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR6481089.1"
                     /translation="MKTQRLFLLIMVCALSACATSPMGRSQLILFPDSEMDQMGVAAY
                     REMQQQTPREQTAGVNDYVQCVVEHVVLAMPDGRMADWEVTVFADDQANAFALPGGKI
                     GVYTGLLDVAENQDQLATVIAHEVAHVLARHSNERVSTTYATQTGLQVVQIAGGTSSP
                     ARNQLYGLLGLGAQVGVLLPFSRAQESEADLMGLDLMAEAGFDPRESVRLWENMAESG
                     GARPPEFLSTHPSGETRMRDLNERMRRAMDLSLQARSAGRNPDCRR"
     gene            5419..5634
                     /locus_tag="AC16PP_05365"
     CDS             5419..5634
                     /locus_tag="AC16PP_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481090.1"
                     /translation="MTDIPDVGAQDPELKNSPIALESDEETVLLREEIPDEPFCYFNS
                     EAFPNGAVVKSGPMKLRCDLGIWVPIG"
     gene            complement(5680..6513)
                     /locus_tag="AC16PP_05370"
     CDS             complement(5680..6513)
                     /locus_tag="AC16PP_05370"
                     /inference="COORDINATES: protein motif:HMM:NF026066.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR6481091.1"
                     /translation="METASREDVNLWVAGSLKEMASLLEDQAANPFRINAYRRAARTL
                     EGLDEPVDALYRRSGREGLMALPSIGEGIATAIENLLRTGRLPQLERLRGSVEPETVF
                     DSLPGVGPRLAAAIHDTLHVDTLEELEVAAHDGRLQTVPGIGPSRAASIRALLAERLK
                     RPPRRALARAPSVAAILDADGQYRREAAAGRLRTIAPRRFNPSHKAWLPVLHTDRNDW
                     HMTLLFSNTARAHELGKTDDWVVVYYYDGDHNEGQCTVVTETAGPRRGARVVRGRENE
                     A"
     gene            6728..7252
                     /locus_tag="AC16PP_05375"
     CDS             6728..7252
                     /locus_tag="AC16PP_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR6481092.1"
                     /translation="MSESQISVESAKPAIRAEQAEPAEESKGLATAPRERDMERLFED
                     FFDKRWLRPWSREWPGFESILEKKGPKVDVVDREHEIFVRAELPGMSKDDIEVSVSDD
                     SLSIKGSSHKEEETEEGDYHRREISTSYVARTVSLPARVRGDDAKATLRDGMLEVTIP
                     KAEDSKRRRVEVES"
     gene            7267..9021
                     /locus_tag="AC16PP_05380"
     CDS             7267..9021
                     /locus_tag="AC16PP_05380"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HIR6481093.1"
                     /translation="MRTDIAREVWEARYRFYRSGEPVDQTIEDTWQRVADTIASRERE
                     PGRWASAYRDLLGDFCFLPGGRILAGAGTGRRVTLCNCFVMGTIEDAMEPIFTALKEG
                     ALTMQAGGGVGYDFSTLRPVGTPARETGNTASGPVSFMHIWDTMCATLCSTGARRGAM
                     MATLRCDHPDIHAFVDAKRDPDALTHFNCSVLVTDAFMNALRDDAEWPLVFPAHHLPT
                     DQGTDIIRREWPGEDGAVPCAVVRRVRARELWEQIMRATYDCSEPGVLFIDRINHENN
                     LSYCERLSATNPCGEVPLPAYGACDLGSFNLTSFVRDPFAAGAAFDFDALRRRVPEAV
                     RFLDSVIDVSGYPLPQQAEQARASRRIGLGFTGLGDALIMLGLHYGSDAARELAAKVM
                     TCIRHEAYAASVALAREKGPFPLFQADAYLQSPGIQRLPESLRRDIARDGIRNSHLTA
                     VAPTGTISLLANNVSSGIEPVFAAEYRREVRGADGTPAVRTVRDFACQLWRDTGRSEA
                     RPPAFVSAEALKPADHLAMQAAVQPHVDHAISKTVNVPADSSFEEFEEIYAEAHRLGL
                     KGCTTYRPGGSRAAVLKH"
     gene            9117..10460
                     /locus_tag="AC16PP_05385"
     CDS             9117..10460
                     /locus_tag="AC16PP_05385"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6481094.1"
                     /translation="MAEETSKHPTPPKTQREFMQKTNREMAAEWAIDVAQDPYSLPIE
                     SLNPAHPALFEANAMLPYFERLRAEAPVHYCEHSQFGPYWSITRFEDVKYVDTHEKLF
                     SSDIRNGGIRLGQQPDPEPDPMFHLPMFIMEDPPKHDEQRKVIAPMFTARHLAGLADL
                     IRERAGRILDGLPVGETFNWVREVSVELTGQMLATLFDVPQEDRHKLIHWSDTVERIG
                     DPDYFETPEQGFQELWKCWEYFDAVWKERQAQSEPGSDLISMLVHGESTKDMPPNEYL
                     GNILLLIVGGNDTTRNSITGGVLALNQNPAEYEKLLADPGLIPNMVPEIIRWQSPVAH
                     MCRTALEDTEVGGRTIRKGDKVAMWYLSGNRDEDVIDDANRFIIDRANARQHLSFGFG
                     IHRCVGNRLGEMQLGIIWEEILKRFARVEVVGEPQYLRSSFIRGIRELPVRVHPA"
     gene            complement(10465..11151)
                     /locus_tag="AC16PP_05390"
     CDS             complement(10465..11151)
                     /locus_tag="AC16PP_05390"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6481095.1"
                     /translation="MAADLLIYGTLGSPFVRKVLVTLQEKGLEWELEEVDVFDPPDWF
                     VALSPARRIPVMRDRSIAAEGVAGTIADSSAICAYVERKHPERPLYPADAYDYARALW
                     FEEYADTLVAARIGFGLLRTLVAAPARGQPPDLERARKAYHEDLPPIFTYLEDVIGDR
                     PFLVGDRFSIADIALASQLAGMRVTRVPIDGGRWPNLADYADRLLARPSFVDRLAEVA
                     SRMPAEDFVP"
     gene            11312..11635
                     /locus_tag="AC16PP_05395"
     CDS             11312..11635
                     /locus_tag="AC16PP_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481096.1"
                     /translation="MKHIVAVLTEPTEGKEDEFNDYYENLHLDEVLATTGWKSAQRFV
                     LTDQIGQKCPLPYLALYEVEADDATEIIPTMDETRPRRQQSDALNRRTAAAWVFSEAG
                     PQHAR"
     gene            11701..12192
                     /locus_tag="AC16PP_05400"
     CDS             11701..12192
                     /locus_tag="AC16PP_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HIR6481097.1"
                     /translation="MSAIDEVLDRNTAFQEGFADAGLQRPPARRIAIVACMDARLDPK
                     GALGLAPGDAHIIRNAGGVITDDAIRSLVVSQRKLGTREIMLIHHTDCGMLNLDEAAF
                     ADEITQETGDKPDFTFHGFADLEGSVRESMQRLQACPFLPHRDAIRGFVYEVESGRLR
                     EVV"
     gene            complement(12209..12559)
                     /locus_tag="AC16PP_05405"
     CDS             complement(12209..12559)
                     /locus_tag="AC16PP_05405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6481098.1"
                     /translation="MIIIAGTIDLADPSKIDEAIEKARPLQQATRDDEPGCHAYVFSP
                     DPCVPGRIAVYELWEDEASLAAHFQHQNYLNMRDLLGTIGLAGADNNKFRCDLKEPVY
                     DETMTPRADFFTAT"
BASE COUNT         2305 a         4268 c         4155 g         2038 t
ORIGIN      
        1 ccaccagggc agccaggacc aggttggcct ggcgccaccc gtaggcggct atgagccagg
       61 tcaccagcag cgggagcacg aagccgccca gggaggtccc caccgaggac aggccgatgg
      121 ccgtgccccg acgggcgaag aaccacttgg ccgccagggt ctgagccgcc agcggtcccg
      181 ccagcagcgc gccggcaacg aacagaaccg aatagatgag gccgatctgc cacagcgcgg
      241 tggcgtgagc ggcaagcgcc agggccgcag cgaaacagac cgcgcccgca accaccagag
      301 cccggatcga caggtggtcc atggcgcggc ccgcgaaggg tgccgcgcag cccatcacga
      361 tctgaacggt gaccaggatg gccatgacct cgccccggct gctgccgaac tccgcgcgcc
      421 acggcgccac ccagaaggtg aagcagaaaa agccgatgcc gaagaccacg gcctggaaca
      481 aaagccccac gcccagcacg ttccagccgt agtaccagcg gggttcggtt cgtgctcgcg
      541 gcgtgccgtc gatgttgcgg gctccccggg gcctgggtca ggcgaccagg cgccggacgt
      601 ccgtggcgga tggcccgccg ggcaggagca tgaccacggc gtcatcgaat ccagcctccg
      661 caaagcggcc gagcaggtcg ccggtttcgc ccaggtcggt gccggctccg agcaggatgg
      721 tggacaccac ggcgcgcctt ccaccggcgg cgcggtagcc atccaacgcc tccaccacct
      781 ggtccggggt gcggtagtgg gcagaagcga tccagccgtc aaatgacgcg gccgctcgct
      841 cgacgccctt gccccacgag cccagcagca gcggcagccc gccgagcacg gccggccagg
      901 gagagagatt ggtctccccc gcacgccctt cctcgaagag ctgcctgagc acctcgaccg
      961 actcgccaaa cgtccggaag cgggaagcgt agtcccggcc gaaggcgtcg aagtccgtct
     1021 gggtcgaccc cgcccccacc ccgagcagca cacgctcgcc ggctacctgc cgcagcgaaa
     1081 gcgcgcggtg agccagatcg accgggtggt aaaggggcac ctgaaccacc gcagtgccga
     1141 gctccacctt ttcggtcacc gccgccgcca cggaaagcgc gacgaagggg tccgtgtaca
     1201 tggcgccacg cccgatggcc tgggccgacc agagactttc gaaaccctcg ccagcgtatc
     1261 ggcgcgcctg atccgccagc gcgttgggct cggacgcgtc gacgattggt cccagcaatg
     1321 ccccaagcct catgacaact cctccttcat tcgaattgat cgctcactca tcgttggcgg
     1381 caccttttaa gcccatttct cgaagcacct cttccgtatg ctcgcccagc atgggcggtc
     1441 ggcggtagag gccggtgggg gtacccgtga acttgatggg ggtgcccacg atggaaactt
     1501 ccccgttttc tcccggcagt tccacctggc tgaccatgcc gcgcgccgcc acgtgggggt
     1561 cggcgaagat gtccgcagcg gtgttgaccg gcccgcacgg caccctgccc ccgagcgcgg
     1621 ccaccacctc ggccctggtg cgcgtcgccg tccagtctga cacaacgccc tccacgaaat
     1681 gctggtgttt gcgccgcacg aacgtgttgc gcgtcttctc gtcgtccacg aggtcgggcc
     1741 ggccgatggc ctgacagagc gcctgccagt gtcccggacc aggagcggcg atggccaccg
     1801 cgccgtccga ggtcgggaaa atgccgaacg ggcagaggtt gggatgatgc gcgccccggg
     1861 ggccaagctc cgtgccggcg tagccgaagt tggctatcac cgtctcggag agaaacatca
     1921 cggcatcgta catccccacg tccatgaact gcccttcgcc cgtccgtctg gcatggtgga
     1981 cggccgcgac cactcccagg gcgagcagag tccccggata gagatcgcct atgctggcgc
     2041 cagccggaaa cccgtgagag ctctcaggcc ccgtgatgtg cgcgatgccg cccatgctct
     2101 gcgccacgat gtcgtaggcg ggccactcgg cgtatggact gcggcccgtg cgcggatccc
     2161 cgaaaccccg gatgcagcca tagaccaggg ccgggttgcg cgcccgcagc acctcgtagc
     2221 cgagcccgag gcgatccatc accccggttc gcatgttctc gatgatggca tccgccttgt
     2281 cgcacaacgc caggagggtc tcgcgccccg ccggggtctt cagatcaatg accacactgc
     2341 gcttgttgcg gttgacgctg gcgaagtagc cgccgaaatc gcagccggcc gactggtccg
     2401 agtttgcgtt ggcgtaatcc tcgggcagcg gcggcacgct tcgcgccaca tccccgcccg
     2461 gcggctccac cttgatgacg ttggcgccga ggtcggcaag aatcgccgca ccaaacggcc
     2521 ccgccagcgc catggtgcaa tcgatgatgg tgatatcggc cagcggccca gagggctcgt
     2581 tcatgcgcca tctctcctcc cggagcaact atacccgcac cgatcgggcc ggcacacctc
     2641 gaagcgcccg tggccgggcg accccgcttt tcgctagaat tttcgatctc gacaaacagg
     2701 cgaaaccgcc gggggaagaa catgggcatg ttggatggca aggtagcgct ggtcaccgga
     2761 gccggtggag ggcttggccg cgcccacgcc ctgctgctgg cgcagcacgg cgctgcggtg
     2821 gtggtcaacg atctgggcgg cgccagggac ggctccggag aggggcacgc catggccgac
     2881 caggtggtgg ccgagattga ggcggcgggc ggcagggccg cagccgacta cgggtcggtg
     2941 agcgatccgg ccgcagcgaa gcagatggtt cagattgccg tggaccgatt cggcaagatc
     3001 gacatctgca tcaacaacgc cggcattttg agagacaaat cgttcaagaa catgaccgat
     3061 gacatgtggg acgtcgtggt ggacgttcac ctgcgcggga cctacctggt cgccaaggcg
     3121 gcctgggacc agatgatcga gcagggaacc ggcggccgga tcatcaacac cagctccacc
     3181 tccgggctca tcggcaactt cggtcaggcc aactacggcg ccgccaaggc gggcatcgcc
     3241 gggctcaccc gggtgctggc gctggagggg atgaaatacg gcataaccgt caacaccctg
     3301 gccccggccg cctggtcacg cctgaccgag gacatcatgc ccgaaggcat ggaggaacgg
     3361 ctggccccgg agaaagtctc tccggccgtg gtctggctgt gcaccgacga cgcagccaac
     3421 gttaccggcc gtcagttctg cgccgggcag aaccgggtct cgctgctctc ctggcaggtg
     3481 accgagatcg ccgacaagaa cgccatggac acgccgtgga cggttgagga gatcggcgag
     3541 cgcatgcagg ccaccatgga cgcctggccc aagctgctca agcccatgga ggtctaggcc
     3601 gcggctacca ggagttcctg agctcgcgga gcttcacgaa cacctcatga ggggtgggct
     3661 cgtccggcag gtccggaaac gcctcccgca ggcggtcgat gaccgtgcgg ctcgtcagcc
     3721 ggaaaaaggt gttgatcttc ttttcctggg ccatggacgt cacgagagcg gcgtccgggt
     3781 cgtgattcgc ctccagctcc ttcagaagcg ctgccgcggc ctcgttgtcg ggctcccggt
     3841 ccagggtgaa ggccaggttg ttggtgatgt agtcatgtcc cggatacacg agggtggttt
     3901 ccggcagctg atcgagctgt gaggcgaacg tctggtagag ctcgtcggga tgccccccgc
     3961 catgacagtt gccggccccg gcgttgaaca gggtgtcgcc acagaacagg gccggcgtct
     4021 ctgtctggga cagcaggcag acgtgggcca ttgtgtggcc cggcgtatcg agcgcaagca
     4081 gctccacggt tctacccacc ttgaccacgt cgcccgcatt gagcgcgacg tccatgccct
     4141 tgatgctgcg ctgggcgttg gcgtgggcca gcagctttgc cccggtggcc tcgatcaccg
     4201 caccgttgcc gccggtgtga tccgggtgct cgtgggtatt gagaacctgg gtgatctgcc
     4261 accccttgtc ccgcgcgcgg gccaggcatt tcttgtggtc cagagggtcg atggccagcg
     4321 cctcgcccga ctccggacag acgatgaggt agttgaagtt tcggtaggag ttaccggtcc
     4381 agatctgctc gacgatcatg cgcgtgcccg tttagtcgaa agcgtcagtg taacccacgc
     4441 acgcgcccgg gacttaccga cgacagtcgg ggttgcgccc cgccgagcgt gcctgcaggg
     4501 agaggtccat ggcgcggcgc atacgctcgt tgaggtcacg catgcgcgtc tccccggagg
     4561 ggtgcgtcga gagaaactcc ggcggccggg cgcccccgga ttccgccatg ttctcccaga
     4621 gcctcacgct ctcgcgggga tcgaagcctg cctcggccat gaggtcgaga cccatcaggt
     4681 ccgcctcgct ctcctgggcc cggctgaacg gcaggagcac gccgacctga gcgccgagcc
     4741 ctagaagccc gtacagctga ttacgcgcgg gagagctcgt cccgccggcg atctgcacga
     4801 cctgcaggcc cgtctgggtg gcgtaggtgg tcgatacccg ctcgttcgag tggcgcgcca
     4861 gcacgtgagc cacttcgtgc gcgatcacgg tggcgagctg gtcctggttt tcggccacat
     4921 cgagcaagcc ggtgtagacg ccgatcttgc cccccggaag ggcgaaggcg ttggcctgat
     4981 catcggcaaa gacggtgacc tcccagtcgg ccatcctgcc gtcgggcatg gccagcacca
     5041 cgtgctcgac cacgcactga acgtagtcgt tgacgccggc cgtctgctcg cggggcgtct
     5101 gctgctgcat ctcccggtag gcggctaccc ccatctggtc catttccgaa tccggaaaca
     5161 ggatgagctg actgcgcccc atgggcgagg tcgcgcaggc ggacagcgcg caaaccatga
     5221 tcaggagaaa gagtcgttgt gtcttcacgg gatgatcccc atgtggcccg cggaaaggat
     5281 gcgcgaactg cccgagagag acaacacgcg cgccaccctc tccgggcatt gtagaatgtc
     5341 catctacggg gttgaccgga tgccattgca ttcgttccgt ccgctagcat gactcgaaag
     5401 ccaacaggac acattccaat gaccgacata ccggacgtgg gcgctcagga ccccgagctc
     5461 aagaactcgc caattgcgct ggagagcgac gaggaaaccg tgctgctcag agaagagatc
     5521 ccggacgagc ccttctgcta cttcaattcc gaagcatttc caaacggcgc cgtggtaaag
     5581 agcggcccga tgaagctgcg ctgcgatctc gggatctggg tgccgatcgg ctagcgctca
     5641 atcgggagcc cgaaagggcg tcgggttcga cgccatgggc taggcttcgt tctcacgccc
     5701 gcgcacgacg cgcgcgcccc tgcgcggccc cgccgtctcg gtgaccaccg tgcactggcc
     5761 ttcgttgtga tcgccgtcgt agtagtagac gaccacccag tcatccgtct tgccgagctc
     5821 gtgggccctg gccgtgttgg agaacagcag cgtcatgtgc cagtcgttgc ggtcggtgtg
     5881 cagcacgggg agccaggcct tgtgggacgg attgaaacgg cgcggggcga tggtgcgcag
     5941 ccggccggct gcggcttcgc gtcgatattg cccgtcggca tccagtatgg cggcgacact
     6001 cggcgccctt gccagggcgc gacgcggcgg ccgcttcagc cgctctgcaa gcagcgctct
     6061 gatactggcc gcccggctgg ggccgatgcc gggaaccgtc tgcagacgac cgtcgtgggc
     6121 ggccacttcc agctcctcca gggtgtccac gtgcagggtg tcgtgaatgg ctgccgccag
     6181 gcgcgggccg acgccgggca gggagtcgaa caccgtctcc ggctccacgc tgccgcgcag
     6241 ccgctcgagc tggggcagcc tgccggtgcg cagcaggttc tcgatggcgg tcgcgatgcc
     6301 ttcgccgatg gaaggcagcg ccatcaaccc ctcgcgcccc gagcgccggt agagcgcatc
     6361 caccggctcg tccaggccct ccaaggtccg cgccgcccgc cggtaggcat tgatacggaa
     6421 cgggttggcg gcctgatcct ccagtaggct ggccatctcc ttcaaagagc cggccaccca
     6481 gaggttcacg tcttcacggc ttgccgtttc cacggtaagg cttcacccgt tgcaactccg
     6541 ggcaatgtga catgcctgcc gggcccgcgt catcaggtat ttcacctaga cggcgcggtc
     6601 ggccggccgc gccgaatcgc cgcccggttc cctcgagaaa accggatccg ggtaagtatt
     6661 tgttcggata cacgtgcctt ccacagcggg cagataatcc cccgtggact gattgaggag
     6721 atgtgaaatg agcgagtctc agatttccgt ggaaagcgcc aagccggcaa taagggccga
     6781 gcaggcggaa cctgccgagg aaagtaaagg gttggcaacg gcaccgcgcg agcgcgacat
     6841 ggaacggctc ttcgaggatt tcttcgacaa gcgctggctg cggccgtgga gccgcgagtg
     6901 gcctggattc gaatcgattc tggaaaagaa gggcccgaag gtggacgtgg tggaccgcga
     6961 gcacgagatc ttcgtccgtg ccgagctgcc gggaatgagc aaagacgaca tcgaggtgtc
     7021 cgtcagcgat gacagcctca gcatcaaggg ctcctcacac aaggaagagg agaccgagga
     7081 gggtgactat catcgccgcg agatctccac gagctacgtc gcccgcaccg tttcgctgcc
     7141 ggcgcgggtt cggggcgacg atgccaaggc gacgctgcgc gacggcatgc tggaggtcac
     7201 gattccaaag gcggaggact ccaagcgccg tcgggtcgaa gtcgagtcgt agccatctcg
     7261 gaccctttgc ggaccgacat cgcgcgggag gtctgggagg cccgctaccg cttctatcga
     7321 agcggcgagc cggtcgatca aaccatcgag gatacgtggc agcgtgtcgc ggacacgatc
     7381 gcttcccggg agcgggagcc gggccgttgg gcttccgctt accgggatct gctgggtgat
     7441 ttctgcttcc ttcccggggg ccggatcctg gctggcgctg gaaccgggcg ccgggtcacc
     7501 ctttgcaact gcttcgtcat gggcaccatc gaagacgcga tggagcccat cttcaccgct
     7561 ctgaaggaag gcgcgctgac catgcaggcg ggcggcgggg tcggctacga cttctccacc
     7621 ctgcgcccgg tgggaacgcc cgcccgggaa accggcaaca cggcctccgg gccggtctcg
     7681 ttcatgcaca tctgggacac catgtgtgcc accctgtgct ctaccggagc ccgccgcggc
     7741 gccatgatgg cgacccttcg ctgcgatcat cccgacatcc atgccttcgt cgatgccaaa
     7801 cgggaccccg acgccctgac ccatttcaac tgctcggtcc tcgttaccga tgcgttcatg
     7861 aacgcgttgc gcgacgacgc cgaatggccc ctggtctttc ccgcccacca cctgccgacg
     7921 gaccagggca ccgacatcat ccggcgcgaa tggcccgggg aagatggagc cgtcccgtgc
     7981 gccgtggtgc gcagggtccg ggcgcgcgag ctgtgggaac agatcatgcg ggcgacgtac
     8041 gactgctccg agccgggggt gctgttcatc gaccgcatca accacgagaa caatctcagc
     8101 tactgcgagc gcctctcggc caccaatccc tgcggcgagg tccctctgcc cgcctacggc
     8161 gcctgcgatc tcggcagctt caacctcacg tcgttcgtgc gagacccgtt cgccgcgggc
     8221 gccgccttcg acttcgacgc cctgcgccgc cgagtgcccg aggccgttcg ctttctcgat
     8281 tcggtcatag acgtttccgg ctatccgctg ccgcagcagg cggagcaggc gcgcgccagc
     8341 cgccgcatcg gcctcggctt cacgggtctc ggcgacgccc tgatcatgct gggccttcac
     8401 tacgggagcg acgccgcgcg cgagctggca gcgaaagtca tgacgtgcat ccgacacgaa
     8461 gcctacgcgg ccagcgtggc gctggcccgg gaaaaggggc cctttccttt gtttcaggct
     8521 gacgcgtacc tgcaatcgcc gggcatccaa cgactgcccg aaagtctccg ccgcgacatc
     8581 gcccgggacg ggatccgcaa cagccacctg accgccgtcg cgcccaccgg aaccatcagc
     8641 ctgcttgcga acaacgtctc cagcggcatc gagcccgtct tcgccgccga gtaccggcgc
     8701 gaggtgcgcg gcgccgatgg cacccccgcc gttcgaaccg tccgtgactt cgcctgccag
     8761 ctgtggcgcg acaccggcag gagcgaggcg cggccgccgg cgttcgtgag cgccgaagcg
     8821 ctcaaacccg ccgatcatct ggccatgcag gcggcggtcc aaccccacgt ggatcacgcc
     8881 atctcaaaga ccgtcaacgt gcccgcagac tcgagcttcg aggagttcga ggagatctac
     8941 gccgaggcgc accgcctggg cctgaagggg tgcaccacct atcggcccgg cggctcccgg
     9001 gcggcggtgc tcaagcattg aaccgaggag cgcggactgc ttaaaaaaat tcgagcgcgc
     9061 ttgccgtcga tcctgccgtt acaatgacgg tttgtgatca ggcagcgtgg acgatcatgg
     9121 ccgaagaaac cagcaagcac cccaccccgc cgaaaaccca gcgggagttc atgcagaaga
     9181 ccaatcggga gatggcggcc gagtgggcca tcgacgtcgc tcaggacccc tactccctgc
     9241 caatcgaatc gctgaacccg gcgcatccgg ccctgttcga ggccaacgcc atgctgccct
     9301 acttcgagcg gttgcgcgcg gaagcgcccg tgcattactg cgagcacagt cagttcggcc
     9361 cctactggtc catcacccgg tttgaggacg tgaagtacgt ggatacccac gagaaactct
     9421 tctcgtcgga catacgcaac ggcgggatcc gcctgggcca gcagccggac ccggagccgg
     9481 accccatgtt ccatctgccc atgttcatca tggaggatcc gccgaagcac gacgagcagc
     9541 gcaaagtgat agcacccatg ttcacggcgc ggcatctggc cgggctggcg gatctgatcc
     9601 gtgagcgtgc cggccgcatc ctggacgggc tgccggtggg ggagaccttc aactgggtcc
     9661 gcgaagtttc cgtggagctc accgggcaga tgctcgcaac cctcttcgac gtcccccagg
     9721 aggatcgcca caagctcata cactggtcag acaccgtcga gcgcatcggc gatccggact
     9781 acttcgagac ccccgagcag ggctttcagg agctgtggaa gtgctgggag tacttcgatg
     9841 ccgtctggaa ggagcgccag gcccagagcg agccgggcag cgatctgatc tccatgctcg
     9901 ttcacgggga atccacgaag gacatgccac ccaacgagta cctgggcaac atcctgctgc
     9961 tcatcgtcgg cggcaacgac accacccgca actccatcac cggcggcgtg cttgcgctga
    10021 accagaaccc ggccgagtac gagaagctgc tcgccgatcc cgggctgata ccgaacatgg
    10081 tcccggagat catccgctgg cagtcgccag tggcccacat gtgccggacg gctctggaag
    10141 acacggaagt cggcggcagg accatccgca agggcgacaa ggtggccatg tggtacctct
    10201 ccggtaaccg ggacgaagat gtcatcgacg atgccaatcg cttcatcatc gaccgggcca
    10261 acgcgcgtca gcacttgtcc ttcggcttcg gcatccaccg ctgcgtgggc aaccggctcg
    10321 gcgagatgca gctgggcatc atctgggagg agatactcaa gcgcttcgcc agggtagagg
    10381 tggtaggcga gcctcagtat ctccgctcga gcttcatccg cggcattcgc gagctgcccg
    10441 tgcgcgtgca cccggcctga tcgctcaggg cacgaagtcc tccgcgggca tacgcgaagc
    10501 aacctctgcc agccggtcta cgaagctcgg ccgggcgagc agacggtcgg cgtagtcggc
    10561 cagattgggc cagcgaccac cgtcgatggg cacccgcgtt accctcatgc ccgccagctg
    10621 cgaagccagg gcgatgtcgg caatagagaa gcggtcgccc accaggaacg gccgatcgcc
    10681 gatgacgtct tcgagatagg tgaagatcgg cggcaggtcc tcgtggtagg ccttgcgcgc
    10741 ccgctcgaga tccggcggct gcccccttgc cggggccgcc accagcgtgc gcagcagccc
    10801 gaacccgatg cgcgccgcga ccagggtgtc cgcgtactcc tcgaaccaca acgcccttgc
    10861 gtagtcgtaa gcatctgccg ggtatagggg gcgctcggga tgtttgcgct ccacgtaggc
    10921 acagatggcg gaggagtccg cgatggtgcc ggcgacccct tcggcggcga tgctccgatc
    10981 ccgcatcacg ggaatgcgcc gggcggggct gagagcgaca aaccagtctg gcgggtcgaa
    11041 gacgtcgacc tcctccagct cccactcgag gcccttttcc tgcagcgtga ccagcacctt
    11101 gcgaacgaag ggggatccca acgttccgta gatgagcaga tcggcggcca ttctcccttc
    11161 cccgcttcgt cccgatgagc aaagagtggc cgctggaccg tggttgtgca agcgctcggc
    11221 tgcgctggaa ctttccgggc cgcaaagttt aagctcgccc tccagcgggc gcgcatccgc
    11281 ccgataatga ccattccagg aggggacgac catgaaacac atcgttgcgg tgctcaccga
    11341 gcccaccgag ggcaaggaag acgaattcaa cgactactac gagaacctgc acctggacga
    11401 agtgctcgca acgacggggt ggaaatcggc ccagcgcttc gtgctcaccg accagatcgg
    11461 gcagaagtgc ccgctgccgt acctggccct ttacgaggtg gaggccgatg acgccaccga
    11521 gatcatcccg accatggacg agacccggcc ccggcgtcag cagagcgatg cgctgaatcg
    11581 ccggacggcg gcggcctggg tattcagcga ggccgggccg cagcacgcgc gctgacggcg
    11641 cctggaaaat ccgcggcgac cgtccgccaa acatcaacag cagaacggga gcgacatcca
    11701 atgagcgcca tcgacgaggt tctagatcgg aacaccgcat ttcaggaagg gtttgccgac
    11761 gcggggctgc agcgcccacc tgcccgccgc atcgccatcg tggcgtgcat ggacgcacgg
    11821 ctcgacccca agggcgccct gggcttggcc cccggtgacg cccacatcat ccgcaacgcg
    11881 ggcggggtga tcaccgacga tgcgatccga tccctggtcg tctcccagcg caagctgggc
    11941 acccgcgaga tcatgctcat tcaccacacc gactgcggca tgctcaacct cgacgaggcc
    12001 gcgttcgccg atgagatcac ccaggagacc ggggacaagc ccgatttcac gttccacggg
    12061 ttcgcggacc tggaaggcag cgtgcgcgaa tccatgcaga gactgcaggc atgccccttc
    12121 ctgccacatc gggacgccat tcgcggcttc gtctacgagg tcgaaagcgg ccggctccgc
    12181 gaggtggttt gagcccccgc agagagcgct aggtcgcggt gaagaagtcc gcccggggcg
    12241 tcatggtctc gtcgtagact ggctctttaa ggtcgcaacg gaacttgttg ttgtccgcgc
    12301 cggccaggcc gatcgtgccc agcagatcgc gcatgttcaa gtagttctgg tgctgaaagt
    12361 gcgcggccag ggacgcttcg tcctcccata gctcgtaaac ggcgatacgt cccggcacgc
    12421 acgggtcggg cgagaacacg taggcgtggc agcccggctc atcatcgcgg gtggcctgct
    12481 gcagaggccg ggctttctcg atggcttcgt cgatcttcga agggtcagcg aggtcaatgg
    12541 ttcccgcgat gatgatcatg tccttctcct taccaaacgc gaaagcatag caaagccacc
    12601 gcgcggccac tatgcacaag cctcacgacc gcgccgtggg caattctcgc caacaggcga
    12661 aacgatgttt caaaaggttt caaataggag tagggttgac gaactgcgtt ggattgggag
    12721 ccagcgtagg acagcgaccc tctttgggga gcgagaaacc attgac
//
LOCUS       DBNHKV010000166         5949 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_165208, whole genome shotgun sequence.
ACCESSION   DBNHKV010000166 DBNHKV010000000
VERSION     DBNHKV010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_165208"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..754
                     /locus_tag="AC16PP_05410"
     CDS             305..754
                     /locus_tag="AC16PP_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6481099.1"
                     /translation="MYKNILVAIDLMEGPAHQVLSGALAFGEETAQYTVVHVVEPQYV
                     QYSFDPTFTGSLTRELEQQAIENATDRMSELCASMEMTPQEQQIVLGRAADQIHELAK
                     HRDADLIVIGSHGRHGWRRLLGSTANAVLHGAPVDVAVIRIPENQDT"
     gene            751..1203
                     /locus_tag="AC16PP_05415"
     CDS             751..1203
                     /locus_tag="AC16PP_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481100.1"
                     /translation="MTDNNSEGRISAFAKDVQRVAEEVEHLGEDTKLVGEEVVGAEIL
                     LTKELTEKIGIAWETAERAGAIGADQVRHLIEDYIATAREFSETPGLHNFLDLTLNHL
                     GRRITHVSEGARAVADLVAEESGRLEDAVLGVWKPFLAVLNRDWESRG"
     gene            1253..2677
                     /locus_tag="AC16PP_05420"
     CDS             1253..2677
                     /locus_tag="AC16PP_05420"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR6481101.1"
                     /translation="MKLLAIDKLQLPAFASTPDGRLVFVNAAWRERFAAGVGDPWHDP
                     FPKVEIDTIGEAWQACHLAHQPFMLEASSPSERYRILCQPVLENGQVLVLGTMADITQ
                     QSRQEAETEAILDTAVDAIIIIDETGRIETFNKAATQLFGYTAAETLGKPVSMLMPEP
                     HRSQHDHYMSRYLTTGEAKIIGIGRDLEAVDQSGRRIPIHLSVSEVQSESGRRFTGII
                     RDLSEQQAARQALAEQREKLAHVGRLSSMGEMTASIAHEINQPLTAISMYAQAGIKLI
                     DRGEPDLARLRDALEKLNTQSLRAGAVIERIQRFARAQKSERIMLDVNHLLDDLGKLA
                     ESDARLYNVELELELAPGLPEVFGDPIQVQQVALNLIRNAIDAMRDIRFVHGRRISLS
                     TSLRSDRMVEIAVSDLGPGVAEDQKELLFTPFHTTKKEGMGMGLSICRSIMTEHGGAL
                     SYRDNDGPGATFFFTLPTWRADDE"
     gene            2670..3293
                     /locus_tag="AC16PP_05425"
     CDS             2670..3293
                     /locus_tag="AC16PP_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6481102.1"
                     /translation="MSEHETTVFVVDDDEPVRDAIGLLLETVEIPYESFPTAQAFLDA
                     YDPSRTGCLVLDIRMPGMSGLELQERLLEDKAPIPIVFITGHGDIPMAVEAMKRGAID
                     FIRKPFRDQELLDRIHAALNADEDQRGAYDNLTTIRERVAALTPREHEVFERVAQGQA
                     NKVVAIELGISERTVEIHRSQVMQKTEARTLADLVRMKLTLDQADGP"
     gene            complement(3371..4411)
                     /locus_tag="AC16PP_05430"
     CDS             complement(3371..4411)
                     /locus_tag="AC16PP_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024779.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein N-acyltransferase Lnb
                     domain-containing protein"
                     /protein_id="HIR6481103.1"
                     /translation="MHSTGKQNFDDLDAEGRIGLVLRRGIRWTVVGFASLWAAGAVYV
                     DGPLGMDDGNIWLAVAWLAVAAACLALIRIAWKKYLVWLVWFLVVLIPWTAKSPSNER
                     PWKPEWAKTGWVEFSDGALEFHNFRNFDYGADGSVVESWETRTVSIRNLRGMDYFHDA
                     FGGDLIAHPMLSFDFGADGYVALSVETRREVDEAYSKLGGLYKMFELQYLWGDERDFV
                     RVRTNVRDEPVYLYRTSFPREQALFVLLDSVRETNLLARQPRFYNVISANCTTSLLAQ
                     TVEFRNAPFDIRMLANGRFDELIYETGGILADGLPFEELRERALINPVALYAHGDAAF
                     SQRIRADRVGFD"
     gene            4610..4960
                     /locus_tag="AC16PP_05435"
     CDS             4610..4960
                     /locus_tag="AC16PP_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6481104.1"
                     /translation="MNANVCVVDGDPAVRDSLATLMRLSGHEVATFATGQEFLDAAEA
                     DTVGCVICEADLPDTTGLAVFGALTEKSLAAPFALLISQSDPARVAQAYRAGIRNVFP
                     KPLVHRRILSFVSR"
     gene            4974..5903
                     /locus_tag="AC16PP_05440"
     CDS             4974..5903
                     /locus_tag="AC16PP_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6481105.1"
                     /translation="MYSNSTTVDSSMELKNILVALHDRESMGSAMRKAARLEHFTGAN
                     ITVVQTCWDVVSEEPTQHFPQEEIDTIASRMKEAELNNLSAELARYSERVAELNAEIL
                     WSKHHDDAVARFAIQSGSDLIVAPRHDRGGVKRLLLPEELKLAARAHAPLLLTSASTW
                     REPTNVLVALDAGDRQHAALNSLLIEWGRTLAETLGGELHLVAASPPPSSSAPNQTLE
                     EFQREAEARRMDALRALADAHPHDYGSLQVKTGDVCDVIDEVASACEVDIVVMGTAAR
                     TGLKRLFIGNSAESVLSQLTEIDILTVPDSQQS"
BASE COUNT         1211 a         1900 c         1832 g         1006 t
ORIGIN      
        1 tcggacgcta cttcccgggc ctgatcgagg acctgtcggg cttcatccgt catgtcgact
       61 gccgccagca cgtgcttgta tgacatttag aggcaccttt gatgaaactc cggacaatct
      121 actcgcgttg ggaccagggc cacatcggga catgtcccct ggaggtttcg ggttcccctt
      181 agtaggaatc ggtcggcatt tgcgcgatgg ttctggaggc tccgtggatg caagttgagc
      241 ctttggtagg atgaagcccg catccgctca ccggggcaga ccctcaatca tcggggatca
      301 ggatttgtac aagaacatac tcgttgccat cgacctcatg gaaggtcctg cccatcaggt
      361 gctgtccggc gcgctggcgt tcggagagga aaccgctcaa tacacggtcg ttcacgtcgt
      421 cgagcctcaa tacgtgcagt acagcttcga cccgacgttc accggatcac tcacccgcga
      481 gctcgagcag caggccatcg aaaacgcaac cgatcggatg tccgagctgt gcgcctccat
      541 ggagatgacg cctcaggagc agcagatcgt gctgggacga gccgcggatc agatccacga
      601 gctggcaaag caccgcgatg ccgatctcat cgtcatcggc tctcacggaa ggcacggctg
      661 gcgccgcctg ctcgggtcca cggccaatgc ggtgctgcac ggtgcgcccg tggacgtggc
      721 cgtcatccgc atcccggaga atcaagacac atgacggaca acaacagtga aggtcgcata
      781 tcagcgttcg caaaggacgt gcagcgtgtc gccgaggagg tggaacatct cggcgaggat
      841 acgaaactgg tcggcgagga agtcgttggc gcggaaatcc tgctgaccaa ggagctgacc
      901 gagaagatcg gcattgcctg ggagacggcc gagcgcgccg gggccattgg cgccgatcag
      961 gtccggcacc tcatcgagga ctacatcgca accgcccggg aattttcgga aactcccggg
     1021 ctccacaact tcctggatct caccctcaac cacctgggaa gacggattac ccatgtaagc
     1081 gaaggcgcca gggccgtcgc cgatcttgtt gccgaggaat cgggacgcct ggaggacgcg
     1141 gtgctgggcg tgtggaagcc gtttctcgcg gtgctcaatc gggactggga gtcgcgcggc
     1201 taggcggatc cgacatgggc agcaccgcaa agctagccat gtccgcggca acgtgaagct
     1261 gctggcaatc gacaagctcc agcttccggc gttcgcgagc acgcccgatg gccgcctggt
     1321 gttcgtgaac gccgcgtggc gggaacggtt cgccgcgggc gtgggagatc cgtggcacga
     1381 tccgttcccc aaggttgaga tcgacaccat cggcgaagcg tggcaggcat gccatcttgc
     1441 tcatcagccc ttcatgctcg aggccagctc gccttcggag cgctatcgca tcctttgcca
     1501 gccggtcctg gagaacgggc aggttctcgt tctcggcacc atggcggaca ttacccagca
     1561 gtcccgccag gaagcggaga cggaggcgat tctcgacacc gccgtcgacg cgatcatcat
     1621 catcgacgag acgggccgga tagagacctt caacaaggcg gccacgcagc tgttcggcta
     1681 cacggccgct gagaccctcg gcaagcccgt cagcatgctg atgcccgagc cccatcgatc
     1741 gcagcacgat cactacatga gccggtacct gacgacgggc gaggcgaaga tcatcggcat
     1801 cgggcgtgat ctggaagccg tggaccaaag cggcaggcgg atacccattc acctctcggt
     1861 gtcggaggtg caatctgaga gcggcaggag gttcaccggc atcatccgcg atctttccga
     1921 gcagcaggcg gcgcgtcagg cgctcgccga gcagcgcgaa aagctggcgc acgtcggtcg
     1981 gctcagctcc atgggggaga tgacggcaag catcgcccat gagatcaacc aaccgctcac
     2041 cgcgatctcg atgtacgccc aggcaggcat caagctcatc gaccggggag agccggacct
     2101 cgcccggctg cgcgacgctc tcgagaagct caacacccag tccttgagag ccggagccgt
     2161 catcgaacgc attcaacggt tcgcacgcgc gcagaaaagc gagcggatca tgctggacgt
     2221 caatcatctg ctcgacgatc tcggcaagct tgccgagagc gacgccagac tctacaacgt
     2281 cgagctggag ctcgagcttg ctcccggtct gcccgaagtc ttcggcgatc cgattcaggt
     2341 tcagcaggtc gccttgaacc tcatccggaa cgccatcgac gccatgcggg acattcggtt
     2401 cgttcacggc cggcgcataa gcctgagcac gtcgcttcga agcgatcgca tggtggagat
     2461 cgccgtgtca gacctcggcc caggggttgc agaagaccag aaggaactgc tatttactcc
     2521 cttccatacg accaagaagg aaggaatggg tatgggttta tcgatctgcc ggtcgatcat
     2581 gaccgagcac ggtggcgccc tgagctatcg cgacaacgac ggacccggag cgaccttctt
     2641 cttcacgctg cccacatggc gcgcggacga tgagtgagca cgaaaccacg gttttcgtgg
     2701 tcgacgacga cgagccggtt cgcgacgcca tcgggttgct gctcgaaacc gtcgaaattc
     2761 cctacgaatc cttccctacg gcccaggctt tcctggacgc ctacgatccg tcccgaaccg
     2821 gttgcctggt gctcgacatc cgcatgcccg gcatgagcgg gctggagctc caggagcgcc
     2881 tgctggagga caaagccccg atcccgatcg ttttcatcac cggccacggc gacattccca
     2941 tggccgtcga ggccatgaag cggggcgcga tcgatttcat tcgcaaaccc tttcgggacc
     3001 aggagctgct cgatcgcata catgcggcgc tcaacgcgga cgaagatcag cgcggcgcgt
     3061 acgacaacct gacgaccatc cgggagaggg tcgcggcgct cactccgcgc gagcacgagg
     3121 tattcgaacg ggtggcccag ggccaggcaa acaaggtcgt cgccatcgag ctgggcatca
     3181 gcgagcgaac cgtggagatt catcgaagcc aggtcatgca gaagaccgaa gctcgaaccc
     3241 tggccgacct ggtgcgtatg aagctgaccc tggaccaggc cgacgggccg tagccggcag
     3301 cagctgaccg gagatcccgg cccggcgacc atcgcggaga gccgttggag cgcggggttc
     3361 cccgaaagga ctagtcgaac ccgacccgat ctgctcggat gcgctgcgag aacgccgcat
     3421 cgccgtgcgc gtagagcgcc accgggttga tcagcgcacg ctccctgagc tcttcaaacg
     3481 gcaggccgtc tgccagaata ccgccggttt cgtatatcag ctcgtcaaag cgtccattgg
     3541 cgagcattcg gatgtcgaac ggggcattgc gaaactccac ggtctgcgcg agcaggctgg
     3601 tggtgcagtt ggcgctgatc acgttgtaga agcgcggctg gcgtgcgagc aggttggtct
     3661 cccgaaccga gtcgagaaga acgaacagcg cctgctcgcg cgggaagctg gtgcggtaca
     3721 gatacacggg ctcatcacgt acgttggtcc gtacccggac gaagtcgcgc tcatcgcccc
     3781 acaggtactg cagctcgaac atcttgtaga gcccgccgag cttcgagtag gcctcatcga
     3841 cctcgcggcg cgtttccacg gacaacgcaa cgtaaccgtc agctccgaaa tcgaaggaga
     3901 gcatggggtg ggctatcagg tcaccgccga acgcatcgtg aaagtaatcc atgccgcgaa
     3961 gattgcgaat actgaccgtg cgggtttccc aggactccac caccgaaccg tcggcgccgt
     4021 agtcgaagtt tcggaaattg tgaaactcga gcgcaccgtc gctgaactcc acccatccgg
     4081 tcttggccca ctcgggtttc cacggcctct cgttggaagg gctcttggcc gtccagggaa
     4141 tcagcaccac tagaaaccag accagccaga cgagatattt cttccaggca atccggatca
     4201 gagccaggca cgccgcggcg accgcaagcc acgcaacagc cagccagatg ttgccgtcgt
     4261 ccatgcctaa cggtccgtcc acgtaaacgg cgcccgcagc ccacaggctg gcaaacccga
     4321 ccaccgtcca cctgatgccc cttctgagca cgagcccgat acggccctcg gcatccaaat
     4381 catcgaagtt ctgcttaccg gtcgaatgca tctgttcgga aagactcacc atctcgaaaa
     4441 caatgcgtcc ctcgcgggaa ggacaccaag ccgccgcgcc ggattctacc agggtcaacg
     4501 gccgggcccg aacgcctccg ggcgatgcgt taggtacata tccgtatttg caggtatcgg
     4561 cccgcgccat aagttatcgg cccagagtca ttcttcgttt cggattgtca tgaacgctaa
     4621 cgtctgcgtc gtcgacggcg accccgccgt acgtgacagc ctggccacat tgatgcggtt
     4681 gagtggtcac gaagtggcga cctttgccac cgggcaggag tttctggacg ccgcagaagc
     4741 cgataccgtg ggctgcgtca tctgcgaggc cgacctcccg gacaccacgg ggctggccgt
     4801 gttcggcgcg ctgaccgaaa agagcctggc agcacccttt gccctgctga tcagccagag
     4861 cgaccccgcc cgagtagcac aagcctatcg cgcgggcatt cgcaacgtgt ttccgaagcc
     4921 gctcgtccat cgccggatac tgtccttcgt ctcgagatga tgggctggca caattgtatt
     4981 ccaactcgac cacggtggat tcgtccatgg agctgaagaa catactggtg gccctgcacg
     5041 atcgggaatc gatgggaagc gcgatgagga aggccgcccg cctggagcac ttcaccgggg
     5101 caaacatcac cgtggttcag acctgctggg acgtggtcag cgaggagccc acccagcact
     5161 tcccgcagga agagatcgac accatcgcct cgcgcatgaa ggaggcggag ctcaacaacc
     5221 tgtcggccga gctcgcgcgc tacagcgaac gggtcgcgga gctgaatgcg gagatcctgt
     5281 ggtcaaagca ccatgacgat gccgtggccc gcttcgccat tcaatcggga tccgacctca
     5341 tcgtcgctcc ccgacacgac cgcggcggtg tgaagcgcct gctgttgccc gaagagctca
     5401 agctcgccgc gcgcgctcat gctccgctcc tgctgacttc cgcttcgacc tggcgcgaac
     5461 cgaccaacgt gctggtcgcg ctggacgccg gggaccgtca gcacgcagcc ctgaacagcc
     5521 tgctcatcga gtggggcagg accctcgcgg aaactctggg gggcgagctt cacctggtgg
     5581 cggcgagccc gccgccgagc tcgagcgcgc ccaaccagac gctggaggag ttccagcgag
     5641 aggcggaagc acgacggatg gacgcgctgc gcgccctggc cgatgctcat ccccacgact
     5701 acgggtccct gcaggtcaaa accggagacg tctgcgacgt catcgacgag gttgccagcg
     5761 catgcgaggt ggacatcgtc gtgatgggca ccgccgcgcg cacgggattg aagcggctgt
     5821 tcatcggcaa ctcggcggaa tccgtgctca gccagctgac cgaaatcgac atcctcacgg
     5881 tgcccgattc ccagcagagc tgatccgcgc gcctgctaaa tcccaggcaa acgaagcgcg
     5941 atcaccttc
//
LOCUS       DBNHKV010000167         4379 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_376976, whole genome shotgun sequence.
ACCESSION   DBNHKV010000167 DBNHKV010000000
VERSION     DBNHKV010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_376976"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="AC16PP_05445"
     CDS             complement(<1..1237)
                     /locus_tag="AC16PP_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6481106.1"
                     /translation="MRFTDVFIQRPVVAIVLGAVMLLLGLQASTQLSLRQFPEVEKSV
                     IFVRTVYPGASARTVQGFVTAPLQRTIAAAKGVEYVTSESNPGSSEIEVHVRLGENST
                     EVLSEVITKVNEARFELPRDVEDPVVTNRTGGDAMMYLALLSDQMSVQQVADYGLRTI
                     QPVLNTVEGVGEARLLGGGLFAMRIWLNPARMAAFGVTATDINDAIRRDNYVSAAGMT
                     HGELVRASVDAQTDVQQPEKFAEIVVRQDEDRRVRLGDVATVELASETYDFAVYSSGK
                     ETVFIAINQAPGSNPLEVAARVKEKVAELEAGLPADLTIFMDSDLSKYIGEALKEVAA
                     TLIEASVIVVLVILLFLGSLRVVAIPIVAIPLSLTTVLFLIWAMGFSINLLTLLAMVI
                     AIGLVVDDAIVVVENVHRHI"
     gene            complement(1240..2310)
                     /locus_tag="AC16PP_05450"
     CDS             complement(1240..2310)
                     /locus_tag="AC16PP_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6481107.1"
                     /translation="MKAYAIVAALLIAIFGAIGGYLYQKFSALASMDFSPPPVTIATS
                     VAQRETWDERLNAVGTVQAVRGVDLTSETSGEIIRINFDSGDAVEAGQLLVVLNDEIE
                     QASRRNQIASLELAEILFERDRALIAQKSIPQSQFDRSRADLERARAQLAETEARLAN
                     KRIEAPFGGTMGIRRVDLGDYLSPGAVIAALQDHSELEIDFTVPARYAPKLRAGLKVA
                     MVVDAFPDREFSAEVVAVDSRIDPSTRNVLLRARLDGAGGLLPGMFATLTVDLGERVD
                     LVTIPETAMTYSLQGNTVYVIEDTEDGGKTAVARVVMSGPVRGGRVAVRSGLQPGEQV
                     VTVGQNKLFRGVRVVIDDAVRF"
     gene            2769..3518
                     /locus_tag="AC16PP_05455"
     CDS             2769..3518
                     /locus_tag="AC16PP_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481108.1"
                     /translation="MPGVFVLLAVPGFNASADSHASPPVQPALEAFFCNYNPGKDRDD
                     LDSATNFYMKQAKKAGFTTPNAFLWTHNKGTAPADIVWMNVHESLVAYGASDDAAAAS
                     SEMADVNDRYDSVATCQAGLGIGTPIITPDPAAAGLEMSTLAAYACTFRHGVGPDALV
                     DLRNHIRETNEGMGDAGLAAAFQIEPMTGGANASDVVFVAVAESTEAWAANLAMLNST
                     PAGQSLVRHFNMVVDCDMNLWWSEQVVGGEG"
     gene            complement(3832..>4379)
                     /locus_tag="AC16PP_05460"
     CDS             complement(3832..>4379)
                     /locus_tag="AC16PP_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6481109.1"
                     /translation="WIRQAISRAIADQARTIRVPVHMIELISKFHRATRQLLQELGRE
                     PSPSEIGALMELSEEQVDRVVKISKHALSLEIPVGDDGDQHLGDLIEDADTASPADLA
                     IDERLKEATQAILGDLTVREAKILRMRFGIDMNTDHTLDEVGKQFNVTRERIRQIEAK
                     ALRKLRHPKFSEQLLGFLRDD"
BASE COUNT          834 a         1374 c         1323 g          848 t
ORIGIN      
        1 caatgtgccg atgcacgttc tccaccacga cgatggcatc gtccaccacc agcccgatgg
       61 ctatgaccat ggccagcagc gtcagcaggt tgatggagaa gcccattgcc cagatcagga
      121 acagcacggt ggtcagcgaa aggggaatcg ccacgatggg aatcgccacg acgcgaagcg
      181 atcccaagaa cagcagaatc accaggacga cgatgacgga ggcttcgatg agggtcgcgg
      241 ccacctcctt cagcgcttcc ccgatgtact tggacagatc ggaatccatg aagatcgtca
      301 ggtctgcggg cagaccggct tccagctcgg cgactttctc cttgacgcgt gcagccacct
      361 ccagaggatt ggagcccggc gcttggttga tcgcgatgaa gaccgtttcc ttgccgctgg
      421 agtaaacggc gaagtcgtag gtttcgctgg cgagctccac ggtagccacg tcaccgagcc
      481 gcacgcgcct atcctcgtcc tggcggacca cgatctcggc aaacttctcc ggctgctgca
      541 catcggtctg cgcatctacg ctggcccgaa ccagctcgcc gtgggtcatg cctgcagcgg
      601 aaacgtagtt gtcgcgccga atcgcatcgt tgatgtccgt tgccgtgacg ccgaaggccg
      661 ccatccgggc cgggtttagc cagatccgca tggcgaacag gccgccgccg agcaggcgcg
      721 cctcaccgac accctccacg gtgttcagga ccggctgaat cgtgcgaagg ccgtaatcgg
      781 ccacctgctg gaccgacatc tgatcgctca gcaatgccag atacatcatg gcgtccccgc
      841 cggtccggtt cgtgaccacc gggtcctcca cgtcccgggg aagctcgaag cgcgcttcgt
      901 tgactttcgt gatcacctcg gagagcactt ccgtggagtt ttcgccgagg cggacgtgca
      961 cctcgatctc ggacgagccc gggttgcttt ccgacgtcac gtactccacg cctttcgcgg
     1021 cagcgatggt tcgttgcagg ggagccgtca cgaacccctg caccgtgcgt gcgctggcgc
     1081 cggggtagac ggtgcgaacg aagatgacgc ttttctcgac ctcgggaaac tgccgcagag
     1141 acaattgcgt gctcgcctga agcccgagca gaagcatgac cgcccccaac acgatcgcga
     1201 ccacgggccg ctggatgaac acgtcggtga agcgcatggc tagaatcgca ccgcgtcgtc
     1261 gatgacgacc cgaacacccc gaaacagctt gttctgcccc acggtgacga cctgctcgcc
     1321 cggctgcaga ccggaccgaa cggctaccct gccgcctctg accggtcccg acatcacgac
     1381 gcgggcaacc gccgtcttgc cgccatcctc ggtatcctcg atgacgtaca ccgtgttgcc
     1441 ctgcagcgag tacgtcatgg cggtctccgg gatcgtcacc agatcgaccc gttcgccaag
     1501 gtctaccgtg agcgtggcga acatgccggg cagaaggccc ccggcgccat cgagccgggc
     1561 ccggagcagc acgttgcggg tcgacgggtc gatcctcgaa tcaaccgcga ccacctcagc
     1621 gctgaattct ctgtccggaa acgcgtccac gaccatcgcg accttcaggc cagctctcag
     1681 cttgggtgcg tatctcgcgg gaaccgtgaa gtcgatttcg agctcgctgt gatcctgcag
     1741 cgccgcgatg acggcgccgg gagagaggta gtcgccgagg tcgaccctgc ggatacccat
     1801 ggtgccaccg aagggcgcct ctatacgctt gttggcaagc cttgcctccg tttccgcgag
     1861 ctgcgcgcga gcacgctcga ggtcggcgcg tgatctgtcg aactgagact gaggtatcga
     1921 cttctgcgcg atgagcgccc gatcccgttc gaagagaatc tctgcgagtt ccaggctggc
     1981 aatctgattg cggcggcttg cctgctctat ctcgtcgttg agcacgacga gcagctgtcc
     2041 ggcttcaacg gcgtcgccgg aatcgaagtt gattcgaatg atctcgccgc tcgtctcgga
     2101 cgtcagatcg acaccccgaa cggcctgcac cgtaccgacg gcgttcaggc gctcatccca
     2161 cgtttcgcgc tgcgccaccg aggtcgcaat ggtgacgggc ggaggggaga agtccatgga
     2221 cgccaaagcg ctgaacttct gatacagata tcccccgatc gccccgaaga tggcaatcag
     2281 caaggcagcg acgattgcat aggctttcaa aggagatctg cccccaacca gacgtccatg
     2341 gacattgtgc atcgcaattc tctgctttca aaccgaccgt gcggcgcagc ctccacaaac
     2401 cagccgctgc ctgctcgtct ttgtgcctcg agcaggctga cgctgtcgcc gtggtcacga
     2461 tcgaagatca caccgatatc agcagtgccg tatcgtcacc gtagttcagt gaatcgcacg
     2521 tgcaagctga tcaacgatcc tcctaggttc cgcgggcgtt gtttacatca tcagcttaac
     2581 gccgcttggc gcgcttcggc tcggtggcct tttgcggcaa attgctggag actatcgtgg
     2641 gggtaagctg atgagtggca ggtgcttcgc acttgctctg attgttgtga ccctcatgag
     2701 cgaaaacaga ccagaataag aggaacgcaa cagtgttaaa aaatatgact caattgcgcc
     2761 gagggctcgt gcctggcgtg ttcgtgctgc tcgccgtccc cggcttcaac gccagcgcag
     2821 attctcacgc ttcgccgcct gtccagcccg ccctggaagc atttttctgc aactacaacc
     2881 cgggaaaaga tcgcgacgat ctcgactcgg ccaccaactt ctacatgaaa caggcaaaga
     2941 aagccggttt cacgacgccc aacgcgtttt tgtggaccca taacaaagga acggctcctg
     3001 cggacatcgt atggatgaac gtgcatgaaa gccttgttgc ctacggtgcg tcagacgatg
     3061 cggccgcggc gtccagcgag atggccgatg tcaatgatcg gtacgattcg gtggcgacct
     3121 gccaggccgg cctgggaatt ggcactccga tcatcacgcc cgatcccgcg gcggccggcc
     3181 tggagatgtc cacgctcgcc gcgtacgcgt gtactttccg ccatggggtc ggacctgacg
     3241 ccctggtgga tcttcgcaac catatccggg agacgaacga aggaatgggt gatgcgggct
     3301 tggccgcggc gttccagatc gagccgatga ccggcggcgc gaacgcgtcc gatgtggttt
     3361 tcgtggctgt cgcagaaagc accgaagcgt gggccgccaa cctggcgatg ctcaacagca
     3421 cgcccgcagg gcaatcgctg gtacgtcatt tcaacatggt cgtcgactgc gacatgaacc
     3481 tgtggtggtc cgagcaggtc gtgggcggag agggctgaag caccgccgga tcatcgtcca
     3541 tcagtaccgg cctcctcggg ggccggtcgt cttgacggac agcgctgcaa gagccctgtg
     3601 ttcgctcctg aacgggggag cgtcggagct attgatatct ctttcgacca gcgggaagac
     3661 gcctattccc acgagcactt cgccgtgcac gatcacgcca ttttgggttt cctcaacacg
     3721 cccggcggcg gcttgagaac cagaagaacg gaagcccagc gttacgctgc ttgacgggtc
     3781 gccagcaccg gatgtgtgag gagacgtcgt cagaccgctc cctgaagtat tctagtcgtc
     3841 ccggaggaag ccgagcagct gctcggagaa tttcgggtgg cggagcttgc ggagcgcttt
     3901 ggcctcgatc tgacgaatgc gctcacgggt cacgttgaac tgttttccaa cctcgtcgag
     3961 cgtgtggtcg gtgttcatat cgatgccgaa tcgcatgcgt agtattttgg cttcgcgtac
     4021 ggtcaggtcg ccaaggattg cttgggtcgc ttccttcagc cgctcgtcga tcgccaggtc
     4081 tgcaggtgat gcggtgtctg cgtcttcgat cagatccccc agatgctgat cgccgtcgtc
     4141 cccgaccggg atttccagag atagcgcgtg cttcgagatc ttcacgacgc gatcgacctg
     4201 ctcttcggac agctccatca gagcaccgat ctcgcttggc gatggctcac gcccgagctc
     4261 ctggagcagt tgacgcgtgg cgcggtggaa tttggagatg agctcgatca tgtgcacggg
     4321 aactctgatg gtgcgtgcct gatcggcgat cgctcggctg atagcctgcc gaatccacc
//
LOCUS       DBNHKV010000168         3097 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1582906, whole genome shotgun sequence.
ACCESSION   DBNHKV010000168 DBNHKV010000000
VERSION     DBNHKV010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1582906"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="AC16PP_05465"
     CDS             <1..802
                     /locus_tag="AC16PP_05465"
                     /inference="COORDINATES: protein motif:HMM:NF018452.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HIR6481110.1"
                     /translation="ITGRRNTIHYLSFGTQIGHYGQGGGMPPTGFIDDKDLEIDPDGS
                     FTILLSPRRQPGNWLPMTEETGTLIVRQTYLDRAAEVPADLSIERVDGGGTPGQVTPA
                     LMDAGLKDASTLVAGASLLFAKWARDFQSHTNRLPMFDQEVSNRAGGDPNITYYHSHW
                     TLAPDEALVIEALPPACEHWNFQLNNYWMESLDYRYHTIHTNKHLATYRPDGSVRIVV
                     AHQDPGVPNWISTVGHASGTMSFRWIRAEATPEPTTRVVRLADVPSLA"
     gene            795..2039
                     /locus_tag="AC16PP_05470"
     CDS             795..2039
                     /locus_tag="AC16PP_05470"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR6481111.1"
                     /translation="MPDFRPPRLPWPVRAFNRMGPALGATGLLPALDAEHMMAEARRR
                     AGLSDFGEDWFRAPLDALLGSINGEARLSALGRTIIRKRIVDALTTRLRARALLDAYP
                     EIESFDPGRVLVIAGLQRTGTTLLHRLLGADPRIRAVLSWEALNPVPLSGPAERERRI
                     RQALRAERGLKYIAPEFFAIHPVEHDMPEEDVLLLDVSFMSQSAEATMHVPTYARWLE
                     EHDHAPAYRYLRQLLSVLLWQRHAPSLVLKSPHHLEHLDTVLDVFPNATIIQTHRDPQ
                     QTVPSFASMVCHGASIFSDRVDVPRLTDHWLRKIRRVVERSLVARERHGTARFVDVSY
                     YDLIGDPMAELARVYEALGLAFDPQAQQGARETLRRQARDRFGRHRYRREDFGLTREL
                     IEGELGFYRRRFDIPFEEEAAS"
     gene            2036..3052
                     /locus_tag="AC16PP_05475"
     CDS             2036..3052
                     /locus_tag="AC16PP_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6481112.1"
                     /translation="MNVKPTTDAPNAVGHRGVVSALVTALSDLVGRRDQLEPMLPDER
                     LDGRTYLVTGASSGLGKAAAVRLASAGAEVIMACRSGIPEAGDDVMRASGSGQVRMLE
                     VDLSDLESASAMCDRLRDDKQHLDGVVFNAGLMPLSARRGPQGFEEMFAVHFLSSRLI
                     VERLLADGVVAPSVDPATRPRLVFVSSEAHRSARPIDFEQFGAFVDYGIRGGMAEYGY
                     SKLHLCTYAVELARRLSSSDGPGVAVHALCPGPVASNIAREAPPWLKPVVGPIIRWLF
                     APSERAVEPVLLLAAGRAVEGRTGVYLHMMREKTFSSLAADPDNGRRLWEASGALIEP
                     FLRR"
BASE COUNT          495 a         1042 c         1030 g          530 t
ORIGIN      
        1 catcaccggc aggcgaaaca cgatccacta cctgagcttc ggcacccaga tcggccacta
       61 cggtcagggg ggcggcatgc cgcccacggg cttcatcgac gacaaagacc tggagatcga
      121 tccggacggc tcgttcacca tcttgctcag ccctcgccgg cagcccggta actggttacc
      181 catgaccgaa gagacgggaa ccctcatcgt ccgtcagacc tacctcgacc gtgcggccga
      241 ggtgcccgcc gatctgtcta tcgagcgggt ggacggcggc ggcacgccgg gtcaggtgac
      301 cccggcactg atggacgcgg gcctcaagga tgcgagcacg ctggtggcag gcgcatcgct
      361 cctgttcgcc aaatgggcga gggatttcca gtcccacacc aaccggttgc ccatgttcga
      421 tcaggaggtc tccaaccggg cgggcgggga ccccaacata acttactatc acagtcactg
      481 gacgctcgcg cccgacgagg cgctggtcat cgaggccctg ccgccggcct gcgagcactg
      541 gaacttccag ctgaacaact actggatgga aagcctcgac taccgctacc acacgattca
      601 cacgaacaag cacctggcca cctaccgccc ggacggctcg gtgcggatcg tggtcgctca
      661 ccaggatccg ggggttccga actggatcag caccgtgggt cacgcttcgg gaaccatgag
      721 cttccgctgg attcgggcgg aggcgacgcc ggagccgacc acccgggtcg tcaggctagc
      781 cgacgtgccg agccttgcct gacttccgcc ctccgcggct cccgtggccc gtgcgcgcct
      841 tcaaccggat gggccccgcg cttggcgcga cggggctcct gccagcattg gatgccgagc
      901 acatgatggc ggaagcgcgt cggcgagcgg gcctttcgga ctttggcgaa gactggttcc
      961 gcgcgccgct ggacgccctg ttgggctcga tcaacggcga ggcccggctt agcgctctgg
     1021 gcagaaccat catccgcaag cgcatcgtcg acgctctcac cacgcgtctg cgcgctcggg
     1081 cgctcctgga cgcgtatccg gaaattgagt ccttcgaccc gggcagggtg ctggtcatcg
     1141 ccgggctgca gcgcacggga accacccttc tgcatcgcct cctcggcgcc gatccgcgca
     1201 tccgggcggt gctctcgtgg gaagcgctca acccggtgcc cctgagcggc ccggcagagc
     1261 gtgagcgcag gatccgccag gccctgcgcg ccgaacgggg actgaagtac atcgcgccgg
     1321 agttctttgc cattcacccc gtcgagcacg acatgcccga ggaagacgtg ctgctgctcg
     1381 atgtctcgtt catgagccag tccgccgagg cgaccatgca cgttcccacc tacgctcgct
     1441 ggctggagga gcacgaccac gcgcccgcct atcggtatct caggcagctt ctgagcgtgc
     1501 tgctgtggca gcgccacgca cccagcctgg tgctcaagtc acctcaccat ctggagcatc
     1561 tggataccgt gctcgacgtt tttccgaacg cgacgatcat tcaaacccac cgcgacccgc
     1621 agcagacggt gccgtcgttt gccagcatgg tctgtcacgg ggcttcgata ttcagcgatc
     1681 gggtggacgt ccccaggctc accgatcact ggctccgcaa gatccgtcgg gtggtggagc
     1741 gctccctggt ggcccgggag cggcacggga cggcgcgctt cgtggacgtc tcctactacg
     1801 acctcatcgg cgatccgatg gctgaactcg ctcgagtcta tgaggcttta ggcctggcct
     1861 ttgaccctca ggcgcagcag ggtgcccgcg agacgttgcg ccggcaggct cgcgaccgct
     1921 tcggccggca tcgctatcgc cgtgaggact tcggtctgac ccgggaactg atcgagggcg
     1981 agctggggtt ctaccgcagg cgtttcgaca tccccttcga ggaagaggcc gcatcatgaa
     2041 cgtcaagccg accacggacg cgcccaacgc cgtcgggcac cgcggcgtcg tgagcgcgct
     2101 tgtcacggcg ctgagcgatc tggtcggacg aagagatcag ctcgagccga tgctgcctga
     2161 tgagcggctg gacgggcgga catatctggt caccggcgcc agcagcggcc tgggcaaagc
     2221 ggcggccgtg cgcctggcga gcgcgggcgc ggaagtgatc atggcctgcc ggagcgggat
     2281 tcccgaagcc ggcgacgacg tcatgcgcgc gtcgggcagc gggcaggtca ggatgctgga
     2341 ggtggatctt tccgacctcg agagcgcctc ggccatgtgc gaccgtcttc gggatgacaa
     2401 gcagcacctc gacggtgtcg ttttcaacgc cgggctcatg ccgctttctg cgcggcgcgg
     2461 gccccagggc ttcgaggaga tgttcgccgt gcactttctg tccagccggc tcatcgtcga
     2521 gcggctgctg gcggacggtg tggttgcgcc gagtgtcgac ccggcgaccc ggccacggct
     2581 cgtgttcgtc tcctccgagg cgcatcggtc tgccaggccc atcgacttcg agcagtttgg
     2641 cgcgttcgtc gactacggaa ttcgcggcgg catggccgag tacggctaca gcaaactgca
     2701 tctgtgcacg tatgccgtgg agctggcccg caggctgagc tcgagcgacg ggccgggggt
     2761 agccgtgcac gccctgtgcc cgggcccggt ggcctcgaac atcgccaggg aagcgccgcc
     2821 ctggttgaag ccggtggttg ggcctatcat ccgatggctg ttcgccccgt ccgagcgcgc
     2881 ggtggagcct gtgcttctgc tcgccgccgg gcgtgcagtg gagggtcgta ccggcgttta
     2941 ccttcacatg atgcgcgaaa agaccttctc atcgctggcc gccgatcccg ataacggccg
     3001 ccgcctgtgg gaggcgagcg gcgccctgat cgagccgttc ctcaggcgct gaggacgaat
     3061 ccctcgtagc cgtgcttggc tgagcggtcg cagtgat
//
LOCUS       DBNHKV010000169         1817 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_165289, whole genome shotgun sequence.
ACCESSION   DBNHKV010000169 DBNHKV010000000
VERSION     DBNHKV010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_165289"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="AC16PP_05480"
     CDS             <1..698
                     /locus_tag="AC16PP_05480"
                     /inference="COORDINATES: protein motif:HMM:NF016086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HIR6481113.1"
                     /translation="IIPRQAVEYIIELHLYARDHLSGALSRSRRAEGLTVVPALIQQI
                     EGFMSANMLHDAHWNFWRLGNFVERADMTTRIIDVGTDNPLQTADAPGPFTDLQWRSV
                     LRSLNATQSYLTVRQEPIHQSAVVGFLIGNRSLPRSLTYSLESLRNGLRTLPRSDRAL
                     RAVNRLRRFLQSSEIENLEGPELHQFLDGCQVQLAEIHQQIDRTYFEFHPRRIPQRAD
                     RSADTQPTRSDST"
     gene            695..1006
                     /locus_tag="AC16PP_05485"
     CDS             695..1006
                     /locus_tag="AC16PP_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6481114.1"
                     /translation="MIRLYGMGRSNYFSIAKACLVEKGMAFEEVATQPSAENAYLAKS
                     PMGKIPCIETERGFLSEAYAIVERIFGKDLLDSIPRARDLLALVGQQPSIQRVEAEKA
                     G"
     gene            complement(1016..>1817)
                     /locus_tag="AC16PP_05490"
     CDS             complement(1016..>1817)
                     /locus_tag="AC16PP_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR6481115.1"
                     /translation="VGVIGQLAERPGSEHGFTWSGGIPFAVLLGISLSGSLKLIVEPR
                     QVSRFFALKDQRSVRVGTWVALGGLTVIMACLFPVGFYAHFLLDGVQDTDMIVPILVS
                     DPNVFPGWVADFLIVAMMAAAMSSMDSVLLVSASVLYKDVVEVVHPAAHPLTWTRIGV
                     VGLALLAAGVALRPPGGIVEITIFSGSLYAVCFFPAILFGLHWRGGSAQAVLASMVSG
                     VLVLVGWLLAGGREFAHEVFPAMLVSCLSYAWVANVTRPALEHWPPEV"
BASE COUNT          400 a          618 c          546 g          253 t
ORIGIN      
        1 ggatcatccc gcggcaggcc gtggagtaca tcatcgagct gcatctgtac gcgcgcgacc
       61 acttgagcgg cgccctgtca cgctcccgtc gagccgaagg gctaaccgtg gtccccgcgc
      121 tgatccagca gatcgaaggc ttcatgtccg cgaacatgct gcacgacgcc cactggaatt
      181 tctggcggct cggcaacttc gtggagcgag ccgacatgac cacccgcatc atcgacgtgg
      241 gcaccgacaa ccctttgcag accgcggacg cgccggggcc gttcaccgac ctgcaatggc
      301 gcagcgtgct gcgctctctg aacgccacgc aaagctacct gaccgtacgc caggagccca
      361 tccatcagag cgccgtggtc ggatttctca tcggcaatcg ctcgctgccg cggtccctga
      421 cctactcgct ggagagcctg cgcaacggtc tgcgcacgct gcctcgcagc gaccgcgccc
      481 tgagggccgt caacaggctg cgcaggttcc tgcagagcag cgagatcgag aacctcgagg
      541 gccctgaact tcaccagttt ctggacggct gccaggtaca gctggcagag atacaccagc
      601 agatcgaccg gacctacttc gagttccatc cacggcgcat accccaacgg gcggatcgtt
      661 ccgcagacac tcaaccaacc aggagcgaca gcacatgatc aggctctacg gcatgggacg
      721 cagcaactat ttcagcatcg ccaaggcgtg tctggtggag aagggcatgg cgttcgagga
      781 agtcgccact cagccgtctg cggagaacgc ctacctcgcc aagagcccca tgggcaagat
      841 cccctgcatc gagacggaac ggggcttcct ttccgaggca tacgccatcg tcgaaagaat
      901 cttcggcaag gatctgctcg actcgatacc gcgggcccgt gacctgctgg cgctcgttgg
      961 ccagcaaccc agcattcagc gcgtagaggc cgagaaagcg ggctgagcga cgacgtcaga
     1021 cctcgggggg ccagtgctcg agggccgggc gggtcacgtt ggcgacccac gcatagctga
     1081 gacacgagac cagcatggcg gggaacacct cgtgagcgaa ctcccttccg ccggcgagca
     1141 gccagcccac cagcacgagc acgccgctga ccatggacgc aagcaccgcc tgggctgaac
     1201 ctccgcgcca gtgcagacca aacagtatgg ccgggaagaa gcagacggcg tagagactgc
     1261 ccgaaaatat cgtgatctcg acgatgccgc ccggcggccg cagcgctacc ccggcagcca
     1321 gcagcgcaag gccgaccaca ccgatgcgcg tccaggtcag cggatgggca gccggatgga
     1381 cgacctcgac cacatccttg tagagaaccg aggcggaaac cagcagaacg ctgtccatgg
     1441 aggacatggc agccgccatc atggcgacga tgagaaagtc cgcgacccac ccaggaaaca
     1501 cattcgggtc gctgaccagg atcggaacga tcatgtccgt gtcctgcacc ccgtcgagca
     1561 gaaagtgggc gtagaaaccc acgggaaaca gacaggccat gatgacggtc aggccgccca
     1621 gcgcgaccca cgtccccacc cgcacgctgc gctgatcttt cagcgcgaag aatcgcgaaa
     1681 cctggcgcgg ctcgacgatc agcttcagag aacccgagag ggaaatgcct aacagaacgg
     1741 cgaacgggat accgccgctc caggtaaaac cgtgctcact ccccggccgc tcggccagct
     1801 ggccaatcac ccccaca
//
LOCUS       DBNHKV010000170         3507 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_165359, whole genome shotgun sequence.
ACCESSION   DBNHKV010000170 DBNHKV010000000
VERSION     DBNHKV010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_165359"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="AC16PP_05495"
     CDS             <1..1507
                     /locus_tag="AC16PP_05495"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase family protein"
                     /protein_id="HIR6481116.1"
                     /translation="SLCAGLAACQDPASGVGPTTGDLEPGRSDRPPNVIILFADDLGY
                     GDLGSYGHPTIRTPRLDALAAAGQRWTDFYAAAPVCSPSRGALLTGRLPNRTGLYGRR
                     LNVLFPNDPGGLPTAERTLAEALGERGYATAVVGKWHLGDRPEAYPTRHGFDYWFGLP
                     YSNDMDWVDAMSFEEVLALAAAGRMPEVEEEIARRQALYLDPRNEYWNVPLVRSQRLA
                     TGITDEVVERPALQASLTSRYTDEAVAFIRRHQAQPFFLYLPYTMPHTPLFPGDAFAG
                     GSRGGRYGDVVEELDWSAGAIADALQELGLAENTLVVFTSDNGPWLAMDHHGGSSGPL
                     RDGKGTTFEGGVRVPAIFSWPGRIKPGVTVGIGSILDLYATALALAGADAGAAGDIDG
                     FDLSGTLLEGRPSPRSSIAYYRSGELMAFRQGEYKLHLVTQGAYGRPPERVVHEQPLL
                     YHLGEDPGERFDAAGRHPDVVKRLQRAVAAHRASLVEQPPLFDRRLQPVHD"
     gene            1530..2315
                     /locus_tag="AC16PP_05500"
     CDS             1530..2315
                     /locus_tag="AC16PP_05500"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6481117.1"
                     /translation="MTDGRLAGRTALVTGASRGIGRAIALAYGREGANVFLTATRLDN
                     LSDTRDRLADMGVEVACHAADVGKPDELETLFAAAAEWRDGVDVLVNNAGIYIGKPFE
                     AYSLEEFDRLMKINVYSVFQLMQLAIRHMTARGRGKIVNIASTAGKWESPNQAAYNTS
                     KHAVVGMTRCAALENASRGLNINAICPGLVETDMLGEFKTHADAQGISFDEFKRAVAA
                     QRIPMGRFLAPEEVAHIAVYLGSGESDGMTGQTITISGGMRMG"
     gene            2359..>3507
                     /locus_tag="AC16PP_05505"
     CDS             2359..>3507
                     /locus_tag="AC16PP_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6481118.1"
                     /translation="MAVQLLADITREHAKDRPDRRAMRYDEDGREWTYAELDADANRV
                     ANALLGDGVEPHGRIAYLDKNAPEYFLYLFGGAKINAVSVAVNWRLAAREMEYILNNS
                     EAGVLVIGEEFLGHLAQMDLPAVTRVVVLGDPGDTGHITFDQWIAGQPDDDPALPAAP
                     GDTCYQLYTSGTTGLPKGVELTHANFIHQVGSALAPLGIRSESVSLVCMPLFHIAGSG
                     FGVLGMYWGADMVLLRDVDLAAIVRVVPEQRVTHAMFVPAVLQFLLAQPGVRDADLSS
                     FETILYGASPITEAVLVEAMDVFGCDFIQGYGLTETTGGVSLLAAEDHDPGGPRAHLL
                     RSCGKVIVNHEVRIVDPESRREMPDGEVGEIWVRGPQVMKGYWKNPEAS"
BASE COUNT          586 a         1138 c         1209 g          574 t
ORIGIN      
        1 gtcgctgtgt gcgggcctgg cagcctgtca ggatccggcg agcggggtgg ggcccacgac
       61 cggtgatctc gagcccggcc ggagcgaccg gcccccgaac gtgatcattc tctttgccga
      121 cgacctcggc tacggagatc tcggcagcta cggtcacccg accatacgga cgccccggct
      181 ggacgcgctg gccgctgccg ggcagcgctg gacggatttt tacgcggccg cccccgtctg
      241 ctcgcccagc cggggcgcgc tgctgaccgg caggctgccc aaccggacgg ggctttacgg
      301 gcgccggctc aacgtgctgt ttccgaacga ccccggcggg ttgccgacag cggagcgcac
      361 cctcgcggaa gccctgggtg agcggggcta cgcgacggcc gtggtcggca agtggcacct
      421 cggagaccgg cccgaggctt accccacccg ccacgggttc gattactggt tcgggctgcc
      481 gtactccaac gacatggact gggtcgacgc catgtccttc gaggaagtgc tcgcgctggc
      541 cgcggcgggt cggatgcccg aggtcgaaga ggagatcgcg cgccggcagg cgctctacct
      601 ggatccacgc aacgagtact ggaatgtgcc gctggtgcgc agccaacgat tggcgacggg
      661 catcaccgac gaggtcgtcg agcggccggc gctgcaggcg tcgttgacaa gccgctacac
      721 ggacgaagcc gtggccttca tccggcgtca tcaggcgcag ccgttcttcc tctacctgcc
      781 gtacacgatg ccccacacgc ctctgttccc gggcgacgct tttgccggcg ggagccgggg
      841 cggtcgttac ggcgacgtgg tggaggagct ggactggagc gcgggcgcga tcgcagatgc
      901 tctgcaagag ctgggtcttg cagaaaacac cctggtggtg ttcaccagcg acaacgggcc
      961 gtggcttgcc atggatcatc acggcgggtc ttcgggtccg ctgcgggacg gcaagggcac
     1021 caccttcgag ggcggtgtgc gggtgccggc gatcttctcg tggccgggcc gcataaagcc
     1081 gggggtgacc gtcggtatcg gctcgatcct ggatctctac gcgacggcac tggccctggc
     1141 tggtgctgac gccggcgccg ccggcgacat cgacggcttc gatctcagcg ggacgctgct
     1201 ggaaggccgg ccgagcccgc gctcgagtat cgcctactat cgcagcggcg agctcatggc
     1261 cttccgccag ggcgagtaca agctgcatct ggttacccag ggcgcctacg gccggccgcc
     1321 cgagcgcgtg gtccacgagc agcccctgct ttaccacctg ggggaggatc cgggcgagcg
     1381 gttcgatgcg gcgggtcgac atccggacgt ggtgaagcga cttcagcgcg ccgtggccgc
     1441 ccaccgggca agcctggttg agcagccgcc gctgtttgat cggcggcttc agccggtgca
     1501 cgattgagca aagcaagggg aggaatcaga tgaccgacgg tcggctcgca ggcagaacgg
     1561 cgctcgtcac cggcgccagt cgtggtatcg gccgcgcgat tgcgctcgct tatggccggg
     1621 aaggcgccaa cgtctttctc accgccaccc gtctcgacaa tctctctgac acccgggatc
     1681 gcctggcgga catgggcgtg gaagttgcct gccacgccgc cgacgtgggc aagcccgacg
     1741 agttggaaac gctctttgcc gccgccgccg agtggcggga cggcgtggac gttcttgtca
     1801 acaacgcggg catctacatc ggcaagccct tcgaggccta cagcctggaa gagttcgacc
     1861 ggctcatgaa gatcaacgtc tactcggtgt tccagctcat gcagctggcc atccggcaca
     1921 tgacggccag ggggcgcggc aagatcgtca acatcgcgtc cacggccggc aagtgggaga
     1981 gtcccaacca ggccgcctac aacacttcca agcacgcggt ggtaggaatg acgcgctgcg
     2041 cggcgctgga gaacgcctcc agggggctca acatcaacgc catctgcccc gggctggtgg
     2101 agacggacat gctgggtgag ttcaaaaccc acgcggacgc ccagggcatc agcttcgatg
     2161 agttcaagcg cgcggtagcc gcccagcgga ttcccatggg acgcttcctc gccccggagg
     2221 aggtggctca cattgccgtc tatctcggca gcggggagtc agacggcatg acggggcaga
     2281 ccatcaccat atccggcggc atgcgcatgg gctgagccgt cgcgttcagc cggatcaacc
     2341 ggatcaagag gagggagcat ggcagttcag ctgttggccg acatcacgcg ggagcacgcg
     2401 aaagacaggc ccgatcgcag agcgatgcga tacgacgagg acgggcgcga gtggacctac
     2461 gcggagctcg acgccgacgc gaaccgcgtg gcgaacgcgc tcctcggtga cggcgtggag
     2521 cctcacggcc gcatcgccta cctggacaag aacgcccccg agtactttct ctacctgttc
     2581 ggcggggcca agatcaacgc cgtctccgta gccgtcaact ggcgcctggc ggcccgggag
     2641 atggagtaca tactcaacaa cagcgaagcg ggcgtgctgg tcatcggtga ggagttcctg
     2701 ggccatctcg cccagatgga cctgccggcg gtgacccggg tggtggtgct gggcgatccc
     2761 ggcgataccg gtcacatcac gttcgatcag tggatcgccg gtcagccaga tgatgacccc
     2821 gcgctgcctg ccgctcccgg ggacacctgc taccagctct acacctcggg aaccacgggg
     2881 ctgcccaagg gcgtggagct gacccatgcc aacttcattc accaggtcgg cagcgccctt
     2941 gcgcccctgg gcattcgatc cgagtccgtc agcctggtct gcatgccctt gttccacatc
     3001 gccggcagcg ggttcggcgt gctgggcatg tactggggag ccgacatggt gctcctgcgg
     3061 gacgtcgatc tggccgccat cgttcgggtg gtccccgagc aacgtgttac ccacgcgatg
     3121 tttgtccccg cggtgctgca gttcctgctc gcccaacccg gggtacgcga tgctgatctg
     3181 tcgtcgttcg aaaccattct gtacggcgct tcgcccatca cggaagccgt gctggtggag
     3241 gccatggacg tgttcggctg cgatttcatc cagggctacg gcctgaccga aaccaccggc
     3301 ggcgtgagcc tgcttgctgc tgaagaccac gatccgggtg gcccgagggc gcatctgctg
     3361 cgctcctgcg gaaaggtcat cgtcaatcac gaggtacgca tcgtcgatcc cgagagcaga
     3421 agggagatgc ctgatggcga ggtgggcgag atctgggtgc gcggccctca ggtgatgaag
     3481 gggtactgga agaaccccga ggcgagc
//
LOCUS       DBNHKV010000171         2344 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1865271, whole genome shotgun sequence.
ACCESSION   DBNHKV010000171 DBNHKV010000000
VERSION     DBNHKV010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1865271"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1837
                     /locus_tag="AC16PP_05510"
     CDS             <1..1837
                     /locus_tag="AC16PP_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6481119.1"
                     /translation="EDGETPYDAAIVGARQVALPVIAMTLTLVAVYLPIGFLGGLTGV
                     LFSEFALTLAGAVLVSGVVALVLSPMMCARLHKDHAHQGRAADWLDARFDRLHRGYER
                     LLSYCLDNRGAVMLFAGAIFLSLPALYLLAQKELAPEEDSGSIFAVAVPPDYGNLEYT
                     TLFLDQMVQAWRTVPEVTHSWQVNEPGSVFGGLELKPWSERERTLEEVRQEVQEKYAR
                     ISGLEIFTFAGGGLPGADSGLPVQFVISSNADYRELDRVSEEVLQLARSSGMFAFVNK
                     DLRYSRPEVNVRINRELAARLGISMEEIGETLQVMLGEAETNRFGVEGRSYKVIPQAD
                     RGFRLTKEWLERYYLRTSNGELVPLSTVISLYQSVEPNALKQFQQLNAVMIQGFVLPP
                     NSLGDALAYLEGALKEVAPTGYRVGYQGESRRYVQETQGFLRLFATSLIFIYLVLAAQ
                     FNSFRDPFIVLISVPLSIFGAVVPLALGFVTLNIYTQVGLLTLIGLISKHGILIVDFA
                     NGRIAEGVDRRQAVIEAAGLRLRPILMTTFATVLGVVPLLNAFGAGANSRFAIGLMIA
                     AGMSVGTLFTLFVLPTFCLLVGRQEGEQPTHHPKSARYVLSEQRR"
     gene            1895..2101
                     /locus_tag="AC16PP_05515"
     CDS             1895..2101
                     /locus_tag="AC16PP_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481120.1"
                     /translation="MVSASGGSLTGQHALVTGGGSGIGLVDTEIAQGLFGTEVVLEDY
                     LACMPLGRTGVVEDVTLGRFDSLK"
     gene            complement(2120..>2344)
                     /locus_tag="AC16PP_05520"
     CDS             complement(2120..>2344)
                     /locus_tag="AC16PP_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6481121.1"
                     /translation="GVGISLPALAYLGFDPTVVNGPDELFSLRAWFSLVPVLFYLASA
                     ALIVGYPITRARHAQIRSELEARKLSGDTG"
BASE COUNT          428 a          687 c          773 g          456 t
ORIGIN      
        1 tgaagatggc gagacgccgt acgacgcggc catcgtcggc gcacgtcagg ttgccttgcc
       61 ggtcatcgcc atgacgctca cgctggttgc ggtttacctg ccgatcggtt tcctgggcgg
      121 cctgacgggg gtgctgttca gcgagtttgc gctgactctg gccggcgccg tgctggtgtc
      181 gggcgtcgtt gcgctcgtgc tgagcccgat gatgtgcgcg cggctgcaca aggatcacgc
      241 gcaccagggc agagcggctg actggctcga tgcccgattc gaccgcctgc atcggggcta
      301 cgagcgcctg ttgtcgtact gcctggataa ccgcggcgcc gtcatgctgt ttgccggcgc
      361 gatctttctc agcctgccgg cgctctatct gcttgcccag aaggagctgg caccggagga
      421 agacagcgga agcatctttg ccgtggccgt tccacccgac tacggcaacc tggagtacac
      481 caccctcttt ctggatcaga tggtgcaggc atggcgaacc gttcccgagg taacccattc
      541 gtggcaggtg aacgagcctg gctcggtgtt cggaggtctc gagctcaagc cctggtcgga
      601 aagggagaga accctggagg aagtgcggca ggaggtacag gagaagtacg ccaggatcag
      661 cggcctggag atttttacct ttgccggtgg tggtctgccc ggcgcggaca gcgggttgcc
      721 cgtgcagttc gtgatttctt ccaacgccga ttaccgggag ctcgaccggg tttcagagga
      781 agtgctgcag ctggcaagga gctccggcat gttcgccttc gtgaacaagg atctgcgcta
      841 ttccagaccc gaggtcaacg tcaggatcaa ccgcgagctg gccgcacggc tggggatctc
      901 catggaggaa atcggcgaga ccctccaggt gatgctgggg gaggctgaaa ccaatcgttt
      961 tggcgtggag ggacgcagct acaaggtgat cccccaggcc gaccggggat tccggctgac
     1021 caaggaatgg ctcgagcgtt actacctgcg cacgtccaac ggcgagttgg ttccgctgag
     1081 cacggtgatc tcgctgtacc aatccgtgga gcccaacgcc ctgaaacagt tccagcagct
     1141 caacgccgtg atgattcagg gattcgttct gccgcccaac agcttgggcg acgcgctggc
     1201 gtatctggag ggcgcgctca aagaggtggc gcccaccgga tatcgggtcg gctaccaggg
     1261 agagtcgcgc cgatacgttc aggagactca gggatttctc cggctgttcg ccacttccct
     1321 gatcttcatc tatctggtgc tggcggccca gttcaacagc ttccgggacc ccttcatcgt
     1381 gctgatcagc gtgccgctat cgatcttcgg cgcggtggtg ccgctggcgc tgggattcgt
     1441 cacgctgaac atctacaccc aggtggggct gctgaccctg atcgggttga tcagcaagca
     1501 cgggatcctg atcgtcgact tcgccaacgg ccgcattgcg gaaggcgtcg acaggaggca
     1561 ggcggtgatc gaggcggcgg gcttgaggct tcgcccgatt ctgatgacca ccttcgccac
     1621 ggtgctgggg gtggtgccgc tgctcaacgc gttcggtgcc ggagcaaact ctcgcttcgc
     1681 gattggcctg atgattgctg cgggcatgtc ggttggaacg cttttcacgt tgttcgtgct
     1741 gccgacgttc tgccttctcg ttggcaggca ggagggcgaa caaccaacac accacccgaa
     1801 atctgctcga tatgttctca gcgagcagcg acggtagccc aggcttcgac gctcttgcta
     1861 gagctgagga ttcgggaatc tacgaaggac agccatggtc tcagcttcag gcggatccct
     1921 gaccggccag catgcgctgg ttaccggtgg cggcagcgga atcgggctgg tggacacgga
     1981 gatcgcccag gggctgttcg gcaccgaggt cgtgctggaa gactatctgg cctgcatgcc
     2041 tctcgggcgc accggggtgg tggaggacgt cacactaggc cgctttgact ctctgaaata
     2101 acgacgagtc tgagtcagcc tagccggtat ccccagacag ctttcgcgct tcgagttccg
     2161 agcggatctg cgcatggcgc gcacgcgtga tgggatagcc gacgatcagc gcggcagagg
     2221 caagatagaa cagcaccgga accagagaaa accaggcacg cagcgaaaat agctcgtcag
     2281 gcccattcac cacggtcgga tcaaagccga gataggcaag cgccggtagc gagatcccga
     2341 cacc
//
LOCUS       DBNHKV010000172        10421 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1725673, whole genome shotgun sequence.
ACCESSION   DBNHKV010000172 DBNHKV010000000
VERSION     DBNHKV010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10421)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10421)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10421
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1725673"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="AC16PP_05525"
     CDS             <1..308
                     /locus_tag="AC16PP_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR6481122.1"
                     /translation="QGALDRGEGGEIAWLAASGLVTTFPLVCFAAAALKLPLSTLGFV
                     QYLAPTITFFLAVLVFGEAFRWEQSVTFGCIWLAVVIFSAEGVYHQRRLQRRLGRWA"
     gene            complement(314..1012)
                     /locus_tag="AC16PP_05530"
     CDS             complement(314..1012)
                     /locus_tag="AC16PP_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481123.1"
                     /translation="MGKVRAMLRLPIMLLFALLVACGRGGILQGGKLEIPQSTGNWFC
                     QPGTGGDEWDCIQDEELARSPQLAAATTPAPPVEESGEGPEAEPPEPAATESPVPRDV
                     EPGAVQEQPPTEEPSAADPEPPKAAEPELDADGLMGLPDDYFAVQLVALDSAAELSAF
                     VDTHGLGYLPHARVERDGRIHHVLLLGFYESYALAEAASLDPPAPIEPGDAWIRPLAG
                     LKRAVERAVSLDAG"
     gene            1232..1822
                     /locus_tag="AC16PP_05535"
     CDS             1232..1822
                     /locus_tag="AC16PP_05535"
                     /inference="COORDINATES: protein motif:HMM:NF025637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR6481124.1"
                     /translation="MIATLPEFDRAPRVLRLNIAGHPVDWVSWQEAVCLYARDIVIWT
                     LGDPILRIRGGHSRLDSSQSSVEIHAIIACDGRVTRPSRSVPPLTNEALFRRDLNVCL
                     YCGGTFADGALTRDHVVPTSRGGRDVWDNCVAACRRCNHHKGSRLAEECGMELLALPY
                     VPNFAEYLALINSGRILGDQMDFLRKSFGSNSRLLQ"
     gene            complement(1839..3296)
                     /locus_tag="AC16PP_05540"
     CDS             complement(1839..3296)
                     /locus_tag="AC16PP_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR6481125.1"
                     /translation="MSDAIAPFVNELWDRSIIPTLSDYVRIPNKSPAFDADWEAHGYM
                     QAAVELLRGWCSVPDIGGLTTEIVTIPGRTPVLFCEVPATAPDAGTVLLYGHYDKQPE
                     FDGWEPGLGPWEPVLRDGKLYGRGGADDGYALFGSLAAIAALQAEGIPHGRCVLLIEG
                     CEESGSFDLPAYVELLEERIGQPDLVICLDAECGNYEQLWTTTSLRGMLPGVLTVKVL
                     SEGQHSGAAGGIVPSSFRILRALYDRVEDADTGSLHDCLYVPIPDDARRQLADVAQVL
                     GATTIDRFPWLDGVTPDGDDLQDLLLANAWEPSLATVGLAGAPDVGNAGNTLRPGTSA
                     KLVFRLPPTLDADEAARTIKAELERSPPNGAHVRFELETPQTGWCAPPVAPWLESALM
                     RASQQYFGKPAMYMGMGGTIPFMKMLGDSFPGVQFVVTGVLGPRSNAHGPNEFLEIAT
                     GKRITACVAAVLAEHARRQARWHARRQARRQARQN"
     gene            complement(3293..5140)
                     /locus_tag="AC16PP_05545"
     CDS             complement(3293..5140)
                     /locus_tag="AC16PP_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6481126.1"
                     /translation="MPMVLATVLALLAPSLSFAEPDPELETITVTAHRIRTDRPVLPV
                     LTRTTGTGPAVGADALRSLPSLAISQAGSLGSVTQVRVRGSEANHLMVLMDGVELADP
                     ATDASFNFANLSLTGTTRVELLAGAHSAIWGSDAVAGVLNFITKPTERRRRLSLEGGS
                     FDTRQAALQLADTTGSYHYNLALSEFRTDGTNIATTGEEDDGFDQSSYRLSGGYRSES
                     WTIEALVHHLKSDADFDPIPFPAFQPRDGDRVSKHDEYLGSVAATWHGDERFEQQIRL
                     SYFDTDNRSFADGARESRTAADRFKATSLTRLRASSRHAVTLMLEHEKERFEQRGAPS
                     IFGDPNQNQSVRTNSLALEYLWAPLDDLSLALSARTDDNSDFENKESYRAAARYRLTG
                     AVELWANWGTGIKNPSFVERFGFTPDTFLGNPDLDPETNRHLSAGAVIGHRQWTLRAT
                     AFRDRLEDEINGFYFDGAAGAFTSINEPGTSKRDGVEVETTLAIPTGVLSMGASWLDA
                     AEPDAAREIRRPQWQAYATVSHERGPFSAELSGYYVGNREDLDFSGFPAERVRLGDYV
                     LLRAHLAYRLGRHLKLALRGENLLDESYQDQLGYASPGRAVYLQVAIDL"
     regulatory      complement(5181..5390)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5393..6535)
                     /locus_tag="AC16PP_05550"
     CDS             complement(5393..6535)
                     /locus_tag="AC16PP_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6481127.1"
                     /translation="MGAGIGGLTLAAALGEAGFRNVTVYDRGEPGSPEPSDLVLSPNG
                     TRVLRALHLGPFLSRAHKPDAYVQRSWRSGFVLAHRPLGAFMEARYGAPEVVVARQAL
                     LEALATRCAHHGVAVRTGQPCARIAQDARSVTLTFGSQEICHDVVIGCDGEASAVRAG
                     MGYAETTQTTQDVVWTGTCPGTDLPVGLRANAVTTWLGPRAYLTQWPSADGGAARFLA
                     FARNPGAGAKLAGAFRNWHEDVFRLVDSATGVTRSAPVEREPIAQWYDRRVALLGDAC
                     HPVTPHLQQGASLAMEDAWVLSRMLERWEDEPAKGFPDYERYRKVRLGRFRQGQRHHL
                     AELTTDPGGKAWRRNLRLSLTSRFLPEIAMQRMDWLYGYDCIKGFE"
     gene            complement(6555..7952)
                     /gene="cysS"
                     /locus_tag="AC16PP_05555"
     CDS             complement(6555..7952)
                     /gene="cysS"
                     /locus_tag="AC16PP_05555"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR6481128.1"
                     /translation="MDIRLHNTKSGRKEAFDPVDPQRITMYVCGPTVYGLVHIGNGRP
                     AVVFDVLYRLLRAAHPEVVYARNITDIDDKINAAARDNDEPIQALADRFSSAYREDVA
                     ALGVLEPTVEPRATHHIAEIIAMIETLIAKGNAYEAQGHVLFHVPSDPEYGSLSHRSL
                     EDMLDGARVEVAPYKQDPKDFVLWKPSTTDLPGWDSPWGRGRPGWHIECSAMISKHLG
                     QTIDIHGGGSDLAFPHHENEAAQSRCANDHPEYVRYWLHNGMLTMGQEKMSKSVGNIV
                     TIRDLLDRFPGEVLRYALLSGQYRSQLAWSEDLLDQAGSSLERLYQALRDAGVEDETS
                     SDYRDLPAEEYPTVVVAALADDLNTPQALAAMHQLASAIHRTSDASEKVELARRLLAG
                     GWLLGILTESAKAHFTAGRDIDAAEIDRLIEERNAARKAKDFARADEIREELTDLGIE
                     LEDTRDGTRWKLRDG"
     gene            complement(7971..8372)
                     /gene="hisI"
                     /locus_tag="AC16PP_05560"
     CDS             complement(7971..8372)
                     /gene="hisI"
                     /locus_tag="AC16PP_05560"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015637550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HIR6481129.1"
                     /translation="MFEYLEDAELGHEVALDALLDAVKFDSDGLVPAIAQDADSRDVL
                     MLAWMNREAVERTLKDGYACYWSRSRGAFWRKGESSGHLQALKEMRFDCDGDAILLIV
                     DQTGPACHTNRPSCFYLSVSDGTARVSSEPV"
     gene            complement(8374..9738)
                     /locus_tag="AC16PP_05565"
     CDS             complement(8374..9738)
                     /locus_tag="AC16PP_05565"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013079.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HIR6481130.1"
                     /translation="MAEALIVSSDAAATPLRLIRTSDFEAFASELSEAERSWIRRAQF
                     AAKSGEFAWLPGADGHPAELVAGWDGGDDLASLGGLPTRIPEGVYELKTEVSDLQLLG
                     WALGSYQFVRYRERDRAPAQLLIPARNDADLVANMDAATTLVRDLINIPAGDMLPSHL
                     ADEAIQVADANQANCRVLVGEQLLEGGFDAIHAVGRAAADAPRLIDITWGDPDHPLIV
                     LVGKGVCFDSGGLDIKPAASMRLMKKDMGGAANALGLAQLIMASNLPVRLRVLIAAVE
                     NAVGANAYRPGDVVPTYKGISVEIDNTDAEGRMVMCDCLALGAEDEPALIVDFATLTG
                     SARSAVGAEIAAMFCNDDEVAAGLSAHGDRWDDPVWRLPLHKPYAYLLDSKVADTVNS
                     ASSPFAGSITAALFLEKFVGSAPWVHFDIMAFNTRPRPGRPEGAEAMAIRAVFGYLQE
                     RFGG"
     gene            9807..10367
                     /locus_tag="AC16PP_05570"
     CDS             9807..10367
                     /locus_tag="AC16PP_05570"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6481131.1"
                     /translation="MLTEVTTHHLELTDPDQFKPAATGPAYALKQVQRPTAELNRFLY
                     STVGAAWFWYERLSWSRARWLEFLDRPEVVTWVAYVDGSPAGYFELEDQPGGSVEICY
                     FGLLPAYIGQGMGGALLTDAIRAAWARGASRVWLHTCSLDHPFALANYLARGFSLFDI
                     VTTTKNLPDPMPEPRPGDAEAGRSSD"
BASE COUNT         1564 a         3423 c         3596 g         1838 t
ORIGIN      
        1 ggcagggtgc cctcgatcgt ggcgaaggcg gcgagatcgc gtggctggct gccagcgggc
       61 tcgtcacgac cttcccactg gtctgctttg cggcggcggc gctaaagctg ccgctgtcga
      121 ccctgggctt cgttcagtat ctggcgccca ccatcacgtt ctttctggcc gtcctcgtgt
      181 tcggcgaggc gtttcgctgg gagcagagcg tcacgttcgg ctgcatctgg ttggccgtgg
      241 tgatcttctc cgccgagggc gtctaccacc agcgcaggct gcagcggcgt cttggccgct
      301 gggcctgacc agttcagcct gcgtccaggg agacggccct ctccacggcg cgtttgaggc
      361 cggccagcgg acgaatccag gcgtcgccgg gctcgatcgg cgctggcggg tcgaggcttg
      421 ccgcctcggc gagggcgtag ctttcgtaga agccgagcag cagcacgtgg tggatgcgcc
      481 catctctctc cacccgggcg tggggcagat acccgaggcc gtgggtatcc acgaacgcgc
      541 tcagctcggc tgcgctatcc agggcgacca gctgaacggc gaagtagtcg tccgggaggc
      601 ccatcagacc atcggcgtca agctcgggtt ccgcagcctt tgggggctcc gggtctgcgg
      661 cggacggctc ttcggtcgga ggctgctcct ggaccgcgcc gggctcgaca tcccgcggaa
      721 ccggcgattc ggtggctgcg ggctctggcg gttcggcttc gggaccctcg cccgactcct
      781 ccacgggggg ggcgggggtc gttgcagcgg ccagctgtgg cgaccgagcc agctcttcat
      841 cctgaatgca gtcccactcg tcgccgccgg tgcctggctg acagaaccag ttgccggtgg
      901 attgcgggat ctccagcttg ccgccctgca ggatcccgcc acggccgcag gccacgagca
      961 acgcgaagag cagcatgatg ggtaggcgca gcatggcgcg tactttgccc accgccgcag
     1021 ttggtgtcaa agctgatctg gcgagtcgag caccgtatgt ccctgggtca cgaagcggcc
     1081 cgaacgctgc tgctggatgc tcgggccagg cgcgggcgcc cgcgggagct ccacgttgaa
     1141 tcgtcaggaa cgattggtca tcataccggc gccgccatcg gggcgagcgt gccccgattg
     1201 cgcatcaatc tgaggttttg agctgccagg catgattgca acgctccccg agttcgatcg
     1261 cgcgcctcgg gtgctgcgac tgaacatcgc cggccatccc gtggactggg tgagctggca
     1321 ggaggcggtg tgcctgtacg cccgggacat cgtcatctgg accctgggcg atcccatcct
     1381 gcgaattcgc ggcggtcact cacggctgga cagctcccag tccagcgtcg aaattcacgc
     1441 catcatcgcc tgcgacggcc gcgtaacgcg gccgtccagg agcgtcccgc ccctgaccaa
     1501 cgaagcgctg ttccgtcggg acctgaacgt ctgcctctac tgcggcggga cgtttgccga
     1561 cggcgctctc acgcgcgatc acgtggtgcc cacttcccgt ggcggacggg atgtctggga
     1621 caactgcgtg gcggcctgca ggcgctgcaa tcatcacaag ggcagccggc tggccgagga
     1681 gtgcgggatg gaactgctgg cgctgcccta cgttccgaac tttgccgagt acctggccct
     1741 gatcaacagc ggccgtatcc tcggcgacca gatggacttt ctgcgaaaga gcttcggctc
     1801 gaacagccgg ctgctgcaat aggcgtcggg gcgtccggct agttctgccg cgcctgccgc
     1861 cgcgcctgcc gccgcgcatg ccaccgcgcc tgccgccgcg catgttcggc cagcacggcg
     1921 gcaacgcagg cggtaatgcg cttgcccgtg gcgatttcta gaaactcgtt cggcccgtgg
     1981 gcgtttgagc gtggcccgag aacgccggtc actacgaact gcacgcccgg gaagctgtcg
     2041 cccagcatct tcatgaacgg gatggtgccg cccatgccca tgtacatggc cggcttgccg
     2101 aagtactgct gggatgcgcg catgagcgcg ctttccagcc acggcgcgac cggcggcgcg
     2161 caccatccag tttgcggagt ctcgagctcg aagcggacgt gggcgccgtt gggcgggctc
     2221 cgctccagtt ccgccttgat ggtccgcgcg gcctcgtctg catccagggt tggcggcagc
     2281 cggaagacca gcttggcgct ggtgcccggt ctgagggtgt tgcccgcgtt ccccacgtcc
     2341 ggagcgccgg caaggcccac ggtggcgagg ctaggctccc atgcattggc cagcagcagg
     2401 tcctgcaggt cgtcgccgtc gggagtgacg ccgtccagcc aggggaagcg gtcgatggtc
     2461 gtggcaccga gcacctgggc gacgtctgcg agctgacggc gcgcatcgtc ggggatgggc
     2521 acatacaggc aatcgtgcag ggaaccggtg tcggcatcct ccacccgatc gtagagcgcg
     2581 cgcaggatcc ggaagctcga gggaacgatg ccgccggcgg ctccggagtg ctgcccctcg
     2641 ctgagcacct tcacggtcag cacgccgggg agcatgccgc gcagcgaggt ggtggtccac
     2701 agctgctcgt agttgccgca ctcggcgtcg aggcagatga cgagatcggg ctggccgatt
     2761 cgctcctcga gcagttccac gtaggcgggc agatcgaagc tgccgctttc ctcgcagcct
     2821 tcgatgagca gcacgcatcg gccgtgggga atgccttccg cctgcagggc ggcaatggcg
     2881 gccagcgagc cgaacagggc gtagccgtcg tcggcgccgc cgcgcccgta cagcttgccg
     2941 tctctgagca ccggctccca agggccgagg ccgggctccc agccgtcaaa ctccggctgc
     3001 ttgtcgtagt gaccgtacag caggacggtc ccggcgtcgg gcgccgtggc cggcacctcg
     3061 cagaacaaca ccggcgtgcg gccggggatc gtgacgattt cggtggtgag gccgccgatg
     3121 tcgggcacgg agcaccaccc tcgcaggagc tcgacggcgg cctgcatgta gccgtgggct
     3181 tcccagtcgg cgtcgaaagc cggcgacttg ttgggaatac gaacgtagtc gctcagcgtc
     3241 gggatgatgc ttcgatccca cagctcgttc acgaacgggg cgatggcgtc gctcataggt
     3301 ctatggccac ttgcaggtac acggcacgtc ctggagatgc ataacctagt tggtcctgat
     3361 agctctcgtc cagaaggttc tcgccgcgaa gcgcgagctt cagatgccgg ccgaggcgat
     3421 aggccagatg ggcgcgcagc agcacgtagt cgcccagccg gacccgctcg gcggggaagc
     3481 cgctgaagtc gagatcctcc cggttgccca cgtagtagcc cgacagctcg gccgagaacg
     3541 gcccacgttc gtggctcacg gtggcgtaag cctgccactg cggcctgcga atctctctcg
     3601 ctgcgtccgg ctcggcagca tccagccagc tcgcgcccat gctcaagacg cccgtgggta
     3661 tcgccagcgt ggtctctacc tctacgccgt cgcgtttgct ggtgcccggc tcgttgatgg
     3721 aggtgaacgc gccggcggcg ccgtcgaagt agaagccgtt tatctcgtct tccaggcgat
     3781 cgcggaaggc ggtggcgcgc agggtccatt gccggtgccc gatcaccgcc cccgccgaca
     3841 gatgcctgtt ggtctcgggg tccagatccg gattgccgag aaacgtgtcc ggggtgaacc
     3901 cgaagcgttc cacgaagctc gggttcttga tgccggttcc ccagttggcc cagagttcca
     3961 ctgcccccgt caggcgataa cgggcagccg cgcggtagct ctccttgttc tcgaagtcgg
     4021 agttgtcgtc ggtgcgtgcg gacagcgcca gggagagatc gtccaggggc gcccaaaggt
     4081 actccagggc gaggctgttc gttcgcacgg actgattctg attcggatca ccgaagatcg
     4141 agggcgcgcc gcgctgctcg aatcgctcct tttcgtgctc cagcatcagc gtgaccgcgt
     4201 gacgcgagct cgcccgcagc cgggtgaggg aagtggcttt gaatcggtcc gcggccgttc
     4261 tgctttcccg ggcaccgtcg gcgaagctgc gattgtcggt atcgaaatag gagaggcgaa
     4321 tctgctgctc gaaccgctcg tcgccgtgcc aggtcgccgc gacgctgccg aggtactcgt
     4381 cgtgcttcga gacgcgatcc ccgtcgcgcg gctgaaacgc aggaaacggg atgggatcga
     4441 agtccgcgtc gctcttcagg tgatgaacga gcgcctcgat ggtccaggat tcgctgcggt
     4501 agccgccgct caagcggtag ctgctctggt cgaagccgtc gtcctcctcg ccggtggtgg
     4561 cgatgttcgt tccgtcggtt ctgaattcgc tgagcgcgag gttgtagtga tacgagcccg
     4621 tcgtgtccgc gagctgcagc gccgcctggc gcgtgtcgaa cgacccgcct tccagggaca
     4681 acctgcgccg gcgctcggtg ggcttcgtga tgaagttcag cacgccggcc accgcgtcgc
     4741 tcccccagat ggcgctgtgg gcaccggcca gcagctcgac gcgcgtcgtg cccgtgagcg
     4801 agaggttggc gaagttgaag ctcgcatcgg tggcagggtc tgccagctcg acgccgtcca
     4861 tgaggaccat gagatggttg gcttcggatc ctcgaacgcg aacctgggtc acggagccca
     4921 ggctgccggc ctggctgatg gcgagactcg gcaggctgcg caaggcgtcg gcgcccacgg
     4981 cgggcccggt cccggtcgtg cgagtcagga cggggagcac cggccggtcc gtgcgtattc
     5041 gatgcgcggt tacggtgatg gtctccagct cggggtcggg ctcggcaaag gagaggctcg
     5101 gggcgagcag ggccagcact gtggccagga ccatgggcat gacggcagac tccggcggcc
     5161 tgcctcaccc cgggcagggt tgctgatacc gggatcgggc cggtctccgg actcgcgagc
     5221 atcaatggcg tgcgccgcct tcccaacgtt tcagcgtcag tggctgagcc tgctcagtgg
     5281 cggctccggc gcggccgtct cgattaccgt tgcgggggca gtgtcggatt tccaccgact
     5341 tcccgttcac ccgatcccac cgcgcggcaa ccgcgcagcg gcgccagtat agctactcga
     5401 accctttgat gcagtcgtat ccgtagagcc agtccatgcg ctgcatggcg atctccggca
     5461 ggaagcggct ggtcagcgac agtcgcagat tgcggcgcca ggctttcccg ccggggtccg
     5521 tggtcagctc tgccaggtga tgccgctgtc cctggcgaaa gcggccgagg cgcaccttcc
     5581 ggtagcgctc gtagtcgggg aaccccttgg ccggctcgtc ctcccagcgc tcgagcatgc
     5641 gcgacagcac ccaggcgtct tccatggcaa gcgacgcgcc ctgctgcagg tgcggggtga
     5701 cggggtggca ggcgtcgccc aggagcgcga cccgccggtc gtaccactgg gcgatgggtt
     5761 cccgttccac cggggcagac cgggtgacgc cggttgccga gtccacgaga cggaagacgt
     5821 cctcatgcca gttccggaag gcgccggcca gcttcgcccc tgctcccggg tttcgggcaa
     5881 aggcaaggaa acgcgcggcg ccgccgtctg cagacggcca ctgggtgagg taggcgcggg
     5941 gtccgagcca ggtggtgacc gcattggcgc gcagaccgac tggcaggtcg gtgcccgggc
     6001 aggtgcccgt ccagaccacg tcctgggtcg tctgcgtcgt ctcggcgtat cccatgcccg
     6061 cgcgaacggc tgaggcctcg ccatcgcagc cgatgacgac gtcgtgacag atctcctggg
     6121 agccgaacgt cagcgttacg cttcgcgcat cctgagcgat gcgggcacag ggttggcccg
     6181 tgcgcacggc gacgccgtga tgcgcgcatc gcgtcgccag tgcttccagc agcgcctggc
     6241 gggcgacgac gacctccggc gctccgtacc gggcctccat gaacgcgccg aggggtcggt
     6301 gcgccagcac gaagccgctg cgccagcttc tctgcacgta ggcatccggc ttgtgggcac
     6361 gagatagaaa cgggcccagg tgcagcgcgc gcagcacgcg ggtgccgttg ggcgacagga
     6421 ccaggtcgga tggctcaggg gagcccggct cgccccggtc gtagacggtg acgttgcgaa
     6481 atccagcttc gccgagggcg gctgcaaggg tcaggccgcc gatgccggca cccacgaccc
     6541 ccaccgatgg cgattcagcc gtcccgcagc ttccagcgtg tgccgtcgcg ggtgtcctcc
     6601 agttcgatgc cgagatccgt cagctcctcg cgaatctcgt ccgcgcgggc gaagtctttc
     6661 gcctttcggg ccgcgttgcg ttcctcgatc aagcgatcga tctcggcggc gtcaatgtcc
     6721 cgcccggccg tgaagtgcgc ttttgcggac tcggtgagaa tgcccagcag ccagccgccg
     6781 gccagcagcc gtcgtgccag ctcgaccttc tcggaagcgt ccgacgtgcg atgaatggcg
     6841 cttgccagct gatgcatggc ggccagggcc tggggcgtgt tcaggtcgtc ggcaagcgct
     6901 gccaccacga cggtcgggta ctcttcggcg ggcaggtcgc ggtagtcgct ggatgtctcg
     6961 tcctccacgc cggcatcacg cagcgcctga tacagacgct ccaggctgct acccgcctgg
     7021 tcgaggagat cctccgacca ggccagctgg gaacggtact gacccgaaag cagcgcgtag
     7081 cgcaggactt cccctggaaa gcgatccaga aggtcgcgga tggtgacaat gttccccacg
     7141 gacttggaca tcttctcctg gcccatggtg agcatgccgt tgtgcagcca gtagcggacg
     7201 tactcgggat ggtcgttcgc gcagcggctc tgagcagctt cgttctcgtg gtgaggaaac
     7261 gccagatccg agccgccgcc gtggatgtcg atggtctggc cgagatgctt gctgatcatc
     7321 gccgagcact cgatgtgcca gccgggccgc ccccgtcccc aggggctgtc ccagcccgga
     7381 agatccgtcg tggaaggctt ccacagcacg aaatccttgg ggtcctgctt gtacggcgct
     7441 acctctaccc gcgcgccgtc gagcatgtcc tcgaggctgc ggtgggaaag gctgccgtat
     7501 tcgggatcgg agggaacgtg gaagagaacg tgcccctgag cctcgtaggc gttgcccttg
     7561 gcgattaggg tctcgatcat cgcgatgatc tctgcgatgt ggtgtgttgc cctcggttcc
     7621 accgtgggct cgagcacgcc cagcgcggcc acgtcttccc ggtaggcgct gctgaaacgg
     7681 tcggccaggg cctggatggg ctcgtcgttg tcccgggcgg cggcgttgat cttgtcgtcg
     7741 atgtccgtga tgttgcgcgc gtagaccacc tcggggtgcg cggcgcggag cagtcggtag
     7801 agcacgtcga acaccacggc cgggcgcccg ttgccgatgt gcaccaggcc gtagacggta
     7861 ggcccgcaga cgtacatcgt tatgcgctgg ggatcgacgg gatcgaaggc ttccttgcgg
     7921 ccggatttcg tgttgtggag tcggatgtcc ataagcggct cgccgtctgc tcagaccggt
     7981 tcggacgaca cgcgcgcggt gccgtcgctg acgctaaggt aaaagcacga gggccggttc
     8041 gtgtggcagg ccggccccgt ctggtccacg atgagcagga tggcgtcgcc atcgcagtcg
     8101 aagcgcatct ccttgagcgc ctgcagatgt ccggaactct cgcccttgcg ccagaaggcc
     8161 ccgcggctgc gagaccagta gcaggcgtag ccgtctttca gggtgcgttc gaccgcctcg
     8221 cggttcatcc aggcgagcat gagaacgtcc ctgctgtcgg cgtcctgggc gatggcgggc
     8281 accaggccgt ccgagtcgaa cttgaccgcg tccagcagcg cgtcgagggc gacctcgtgg
     8341 ccgagctccg cgtcctcgag gtattcgaac atctcagcct ccgaatcgtt cctgcaggta
     8401 gccgaaaacg gccctgatcg ccatggcttc cgcaccctcg ggtcggcccg ggcgggggcg
     8461 tgtgttgaag gccatgatgt cgaagtgcac ccacggcgcc gagcccacga acttctccag
     8521 gaagagggcg gccgttatgg atccggcgaa cggcgagctt gccgagttga cggtgtcggc
     8581 caccttgctg tcgagcaggt aggcgtaggg tttgtgcagc ggcagccgcc acacagggtc
     8641 gtcccagcgg tcgccgtggg cgctcagccc ggcggcgacc tcgtcatcgt tgcagaacat
     8701 cgccgcgatc tctgcgccca cggccgatcg cgcggatccg gtcagcgtgg cgaaatcgac
     8761 gatgagggcg ggttcgtcct cggcacccag cgccaggcag tcacacatga ccatgcgccc
     8821 ttcggcgtcg gtattgtcga tttccaccga gatgcccttg taggtcggca cgacgtcgcc
     8881 aggccggtaa gcgttcgcgc cgacggcgtt ctccactgcc gcaatgagca cgcgcagacg
     8941 aacgggcaga ttgctcgcca tgatgagctg ggcgaggccg agagcgttgg ctgccccgcc
     9001 catgtccttt ttcatgagcc gcatactggc ggccggcttg atgtcgagcc cgccgctgtc
     9061 gaagcacacc cccttgccca ccagcacgat cagcggatgg tccgggtctc cccaggtgat
     9121 gtcgatcagg cgcggcgcgt cggccgccgc ccgcccaacg gcgtgaatcg cgtcgaagcc
     9181 gccctccagc agctgctcgc ccaccagcac ccggcagttt gcctggttgg cgtccgccac
     9241 ctggatggcc tcgtctgcga gatggctcgg cagcatgtcg cctgcgggga tgtttatgag
     9301 atcgcgcacc agggtagtgg ccgcgtccat gttcgcaacg aggtccgcgt cgttgcgtgc
     9361 aggaatcagg agctgggcgg gagctctgtc gcgctcccgg tagcggacga actgatagga
     9421 accgagcgcc cagccgagca gctgcaggtc ggagacctcg gttttcagct cgtacacgcc
     9481 ttcgggtatg cgggtgggaa ggccgcccag gctggccagg tcgtccccgc cgtcccatcc
     9541 tgccaccagc tcggccgggt ggccgtcggc tccgggcagc caggcgaact cgccgctctt
     9601 cgccgcgaac tgagcacgcc ggatccagct ccgttcggct tccgaaagct cgctggcaaa
     9661 cgcctcgaag tccgaggttc tgatcagcct gagcggcgtt gcggcggcgt cggatgaaac
     9721 gatgagcgct tctgccattg gggctccggt tggttgaagt ccgagatggt ctatgatggc
     9781 ctcccggttc gcaagcggtc tgagccgtgc tcacggaagt caccacgcat cacctcgagc
     9841 tcaccgatcc ggatcagttc aagccggcgg ccaccggtcc ggcctacgcg ctcaagcagg
     9901 tccagcgtcc caccgcggag ctgaaccgct ttctctactc gacggtcggg gcggcgtggt
     9961 tctggtacga gcgcttgagc tggtcccggg cccgctggct cgagtttctc gaccggccgg
    10021 aggtcgtcac ttgggtcgcc tatgtggatg gcagccccgc cggctatttc gagctggagg
    10081 atcagccggg cgggtcggtg gagatctgct atttcggcct cctgccggcc tacatcggtc
    10141 agggcatggg cggcgctctg ctgacggatg ccatccgcgc cgcctgggcc aggggcgcgt
    10201 cccgcgtctg gcttcacacc tgctcgctgg atcacccttt tgcgctcgcc aactatctgg
    10261 cgcggggctt ttccctgttc gacatcgtca cgaccacgaa aaatctgccc gatcccatgc
    10321 cggagccccg gcccggggat gccgaggcgg gccggtcgtc agattgattt gaccgggatc
    10381 cgcccgagcc cgaggttgtt gccttcgagt atgcggtcgg c
//
LOCUS       DBNHKV010000173         4163 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1016959, whole genome shotgun sequence.
ACCESSION   DBNHKV010000173 DBNHKV010000000
VERSION     DBNHKV010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1016959"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3076)
                     /locus_tag="AC16PP_05575"
     CDS             complement(<1..3076)
                     /locus_tag="AC16PP_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/M1 family
                     aminopeptidase"
                     /protein_id="HIR6481132.1"
                     /translation="MFLEVFRFENRFQVRSPLFLTISTVWFLFGFLITGSESVSVGGV
                     GSNLNLNASFAIIQTITVLTIIGMFAAIAFVASAITRDRETKTAEIFYSSGISERSYL
                     LGRFAGGCVFATAAVTAGVVGTMLGTFMPWLDAERIGSFSLAPYVFSMLVIVLPNMFI
                     ICALFFSVAALTRSMMASYVAALGFLVSFAVIGSATDPENIRYLALGEPFGQVALGEI
                     TRYWTVFDRNFRVPELAGTLLLNRVIWVLVATAVLGITVARFAFELKPARKRWWRRRP
                     NRSEARAAPRHVVDVARASAGMTASLLSQLRMDFRGIIRSVPFYVLLAFGMLNVIGGF
                     YGAVSQWYGTEVYPVTRIMTQVVGSTFAFVVLLILIYYSGELVHRERQSGVADYLDAA
                     PYPNGIMILSKVFGLWLVISLLYLVVVLAGMLVQALNGYREFEPLLYLVSVFGVIGGS
                     FYLWAVPAVLIQSLASNKFLGMAALLVIFLGLTTLEGFGFEHMLYNFGLPAAPYSDMN
                     GYGHFVGRIVSAFAYWASLCVILVVVAHLFYPRGLQTAAERLATARQRFSGGVGAVVV
                     LAALTFCGLGGWIFYNTNVLNEYLTSEDLEEAQADYEKAYRQHRRLPPLQVVDLNLAV
                     DVFPDERRLESRGTARLINANEQPVSDVHLTLPPLLRVNELSLERAGLVAEDERLGYY
                     QYVLDEALPPGAETTLSWDLSWINEGFPNSGATTRVVGNGTFVDSTEIAPTLGYDSGR
                     ELNDNNVRREYGLPPVERLPKLGDPDWLGTNQLGVSVRSDFRAVVSTSADQIAVAPGY
                     LQREWVEDDRRFFEYAMDQPIWPFVSVSSARYVVARDKWDDVDLEVYHDPKHPYNVET
                     MITATKKSLDYFTREFSPYQYRQFRILEFPAYASFAQSFPNTIPYSEGIGFIADLRDP
                     AEIDYVFYVTAHELAHQWWAHQVVGAQMQGMTVIVETLAQYSALMVMEQEYGPERMRR
                     FLKYELDNYLQSRGGELIEELPLLYVENQGYIHYSKGSVVMYALKDAIGEDKV"
     gene            complement(3088..3975)
                     /locus_tag="AC16PP_05580"
     CDS             complement(3088..3975)
                     /locus_tag="AC16PP_05580"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017737462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:0140657 - ATP-dependent activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6481133.1"
                     /translation="MLKINALSKTYPNGVQALDGVDLEIAKGMFGLLGPNGAGKSTLM
                     RTIATLQSPDSGSIHFNDVDVLADPNALRRVLGYLPQEFGAYPRTSPEVMLDHIAALK
                     GIGGRGARKALVEQLLVQTNLWDVRKKSIDSFSGGMRQRFGIAQALIGDPQLVIVDEP
                     TAGLDPAERRRFHNLLARIGEEVVVILSTHIVEDVAELCPNMAVMGGGRILVQGEPKE
                     LTGRLAGRLWSKVVPRDEAEGLRRSLPVVSTRLVAGQVELRVLSEQDPEGGLAAVEPS
                     LEDVYFATLMKHGMNPVVD"
     gene            complement(3969..>4163)
                     /locus_tag="AC16PP_05585"
     CDS             complement(3969..>4163)
                     /locus_tag="AC16PP_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481134.1"
                     /translation="EPLAAPVRSLRYSRFANARRQGGGAHALRLAGMVELLVERAAPG
                     ARWFSGVEERPIELCKEKAC"
BASE COUNT          842 a         1364 c         1269 g          688 t
ORIGIN      
        1 tgaccttgtc ctcgccgatg gcatccttga gcgcatacat caccacgctg cccttgctgt
       61 aatggatgta cccctggttc tccacgtaaa gcagaggcag ctcctcgatc agctcaccgc
      121 cgcgggactg cagatagttg tccagctcgt acttgaggaa acggcgcatg cgctcgggcc
      181 catactcctg ctccatcacc atcagcgccg agtactgagc cagagtctcg acgatgacgg
      241 tcatcccctg catctgcgcc ccgaccacct gatgcgccca ccactggtgg gcaagctcgt
      301 gcgcggtcac gtagaacacg taatcgattt ccgcgggatc gcgaagatcg gcgatgaacc
      361 cgatgccctc ggagtaggga atcgtgttcg ggaacgactg tgcgaacgac gcgtacgcgg
      421 gaaactccag gatccggaac tggcggtact ggtaggggga gaattcccgg gtgaagtagt
      481 ccagcgactt cttcgtggcg gtgatcatgg tctcgacgtt gtacgggtgc ttggggtcgt
      541 ggtacacctc taagtccacg tcgtcccact tgtcgcgggc cacgacgtat cgcgcggacg
      601 agacggacac gaagggccag atcggctgat ccatcgcgta ctcgaagaag cgtcgatcat
      661 cttccaccca ctctcgctgg agatagcccg gggccacggc aatctgatcg gccgaggtgc
      721 tgaccacggc gcgaaagtcg ctgcgaacgc tgacgccgag ctggttggtc cccagccagt
      781 ccggatcgcc cagcttggga aggcgctcca cgggcggcag cccatactcg cgacggacgt
      841 tgttgtcgtt tagttcccgg ccgctgtcgt agccgagcgt cggggctatc tcggtgctgt
      901 cgacgaaagt gccgttgcca accacccgag tcgtagcgcc cgaattcgga aaaccctcgt
      961 tgatccagga caggtcccag gaaagcgtcg tttccgctcc cggcggcaac gcttcatcga
     1021 ggacgtactg gtagtagccg agccgctcgt cttcggcgac gagcccggct cgctctaggg
     1081 acagctcgtt gacccggagg agcggcggca gcgtaaggtg aacgtcgctg accggctgct
     1141 cgttggcgtt gatcagacgc gcggtgcccc tcgactccaa ccggcgctca tcgggaaaca
     1201 cgtcgaccgc taggttcaga tccacgacct gcaaaggcgg cagccgacgg tgctgtcggt
     1261 aagccttttc gtaatccgcc tgggcttcct ccaggtcctc ggaggtcagg tactcgttca
     1321 agacgttggt gttgtagaag atccaacccc ctaggccgca gaaggtgagg gcggcgagga
     1381 ccaccacggc gccaaccccg cccgagaaac gctgacgggc cgtcgccagc ctctctgccg
     1441 ccgtctgcag gccgcgggga tagaaaagat gggcgacgac gacgaggatg acgcacaagc
     1501 tcgcccagta cgcaaacgcc gaaacgattc gcccgacgaa atgcccgtag ccgttcatgt
     1561 ccgaataagg tgcggcgggc agcccgaagt tgtagagcat gtgttcgaag ccgaaaccct
     1621 ccagggttgt caggcccaga aagatcacca gcagcgccgc catgccgaga aacttgttcg
     1681 acgccagcga ctggatgagc accgccggga ccgcccacag atagaacgaa ccgccgatca
     1741 cgccgaacac gctgacgaga tagagcaacg gctcgaactc ccggtagccg ttgagcgcct
     1801 ggacgagcat gccggcaagc acgaccacca ggtacagcag gctgatcacc aaccagaggc
     1861 cgaacacctt ggagaggatc atgatgccgt tgggatacgg cgcggcgtcg aggtagtcgg
     1921 ccaccccgct ctggcgctcg cgatgcacca gctcgccgga atagtagatg aggatcagca
     1981 gcaccacgaa cgcaaaggtg ctccccacca cctgagtcat gatgcgggtg acggggtaaa
     2041 cctccgttcc gtaccactgg ctgacggctc cgtagaaacc accgatcacg ttcagcatcc
     2101 cgaaggccag cagcacgtaa aagggaacac tccggatgat cccccggaag tccatgcgca
     2161 gctgcgagag gaggctcgca gtcatcccgg cacttgcccg ggcaacgtca accacgtgac
     2221 ggggcgcggc tcgcgcctcg gaccggttcg gccggcgtcg ccaccaccgc ttgcgggcag
     2281 gcttcagctc gaaggcaaat cgggccaccg tgatccccag gacggccgtc gcgacgagga
     2341 cccagatcac gcggttgagc agcagcgtcc ccgcgagctc cggaacccgg aagttccgat
     2401 cgaacactgt ccagtatcgg gtgatttctc ccagcgccac ctgtccgaac ggttctccga
     2461 gcgccagata ccggatgttc tcaggatcgg tggcgctgcc gatcaccgcg aaagagacca
     2521 gaaacccgag cgcggcgacg taggacgcca tcatcgatcg ggtcagagcc gccacgctga
     2581 agaacaaagc gcagatgatg aacatgttcg gcagcacgat gaccagcatg ctgaacacat
     2641 aaggcgccag gctgaagctg ccgatacgct ccgcatcgag ccagggcatg aaggtgccca
     2701 gcatggtccc gacgacgccg gcggtcaccg cggcggtggc gaagacgcac cccccggcaa
     2761 atcgtcccag cagatagctc ctctcggata tcccgctgct gtagaagatc tcggccgtct
     2821 tggtttcgcg atcgcgcgtg atggcgctgg ccacgaaggc tattgccgca aacatgccga
     2881 tgatggtgag caccgtgatc gtctggatga tggcaaacga cgcgttcagg ttcaggttgc
     2941 tgccgacacc gccgacgctc accgattcgc tgccggtgat cagaaaacca aaaaggaacc
     3001 acaccgtgga tatcgtcagg aacagcgggg agcggacctg aaagcggttt tcgaatctga
     3061 agacctcgag aaacatggcg cctcctctca gtcgaccacc gggttcatgc cgtgcttcat
     3121 gagggtggcg aagtaaacgt cctccaggct cggctcgacc gcggcgaggc caccctcggg
     3181 gtcctgctcc gagagcaccc ttagttccac ctgccccgcc accagtcgcg tcgacaccac
     3241 cggcagggaa cgccgcaacc cttcggcctc gtcgcgggga accactttcg accacagccg
     3301 tcccgcaaga cgcccggtca gctccttggg ttcaccctgc accagaatcc gcccgccgcc
     3361 catgacggcc atgttcgggc aaagctcggc aacgtcctcg acgatgtggg tggacagaat
     3421 gaccaccacc tcctcgccga ttctggccag caggttatga aagcgccggc gctctgcggg
     3481 atcgagcccc gccgttggct cgtcgacgat caccagctgc ggatcgccga tgagcgcctg
     3541 ggcgatcccg aagcgttggc gcataccccc ggaaaagctg tcgatgctct tcttgcgcac
     3601 gtcccagagg ttcgtctgca ccagcagctg ctcgacgagc gctttgcgag cgccgcgacc
     3661 gccaatgccc ttgagcgctg cgatgtgatc gagcatcact tccggagacg tgcgcggata
     3721 cgcgccgaac tcctgcggga ggtagcccag cacgcgacgc agggcgttgg gatccgcgag
     3781 cacatcgacg tcgttgaagt gaatggaacc gctgtccggg ctctgcagcg tcgcgatggt
     3841 gcgcatgagc gtggacttgc cggcaccgtt cggcccgagc aacccgaaca tgcccttggc
     3901 gatctccaga tccacgccgt ccagcgcctg cacaccgttg ggataggtct tcgacaaagc
     3961 gtttatcttc agcatgcttt ctccttgcag agctcgatcg gtcgttcctc gacgcccgag
     4021 aaccaccgcg ctcccggcgc ggcccgttcc accaacagtt cgaccatgcc ggccaaccgc
     4081 aatgcgtgcg cgccaccacc ttgccggcgc gcgttggcga atcgagaata cctcaatgag
     4141 cgaactggcg cggcaagcgg ctc
//
LOCUS       DBNHKV010000174         1945 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1299326, whole genome shotgun sequence.
ACCESSION   DBNHKV010000174 DBNHKV010000000
VERSION     DBNHKV010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1299326"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="AC16PP_05590"
     CDS             complement(<1..348)
                     /locus_tag="AC16PP_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6481135.1"
                     /translation="MAGTSVADSALRQWLDIQSIRNVLAIHSRGIDRADGDLLRSAYH
                     PDAEVAYGFFDGPAAELCEIMGNPSEQITMHRTSNMWIRLLDDDHAISESYAFVYSES
                     ADDAGYGQALIGGR"
     gene            450..977
                     /locus_tag="AC16PP_05595"
     CDS             450..977
                     /locus_tag="AC16PP_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6481136.1"
                     /translation="MSDRVSSYRFFQEAYLVNDLDQAIRGWSDLYGAGPFRTVMHHKT
                     DRFHYRGTDQEADVSYAFGYLGDMQIQFIEQHDDTPSIYRDMYPAGEEGFHHVAILVH
                     DFEAEYERLVGKGFTDACRLFADGVDAAYFDTRSVNGCFTEIHGDPPRNLGRWAQMRR
                     AHELWRPGDDVYMTL"
     gene            1135..1749
                     /locus_tag="AC16PP_05600"
     CDS             1135..1749
                     /locus_tag="AC16PP_05600"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6481137.1"
                     /translation="MLRRVAASLRRPVASLCAILFVSGPALAAEIEEIVVTARGVEQS
                     VRDIPVAISVVSEERMANLNLKSLEDIAEVTPQLNIVRASSGSGTSISIRGIGSSPST
                     IGIEQSVSTIVDGVYYPQGRVINEAMFDVRQVAILKGPQALYFGKNATAGVISVETNN
                     PGDELEIIGRVGYEFEQERMSTKASSPARSPRPWARGLHCAIPT"
     gene            1746..>1945
                     /locus_tag="AC16PP_05605"
     CDS             1746..>1945
                     /locus_tag="AC16PP_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481138.1"
                     /translation="MNEGYISNPVGTTTYTTLDQAFSPPVATVLENPPPDRDKWPGTE
                     SFYGRLTLAWDPNDQFGLNIKGS"
BASE COUNT          359 a          643 c          607 g          336 t
ORIGIN      
        1 acggccgccg atgagcgcct gcccgtagcc ggcatcgtcg gccgattccg agtagacgaa
       61 cgcatagctc tcggagatgg cgtgatcgtc gtccagcagc cggatccaca tgttgctggt
      121 gcggtgcatg gtgatctgct cgctgggatt gcccatgatc tcgcagagct cggcggccgg
      181 gccgtcgaag aacccgtagg cgacctcggc gtccggatgg taggcggaac gcagcagatc
      241 gccgtcggcg cgatcgatgc cgcggctgtg gatcgccagc acgttgcgga tggattgaat
      301 gtcgagccac tggcgcagag ccgagtctgc gacgcttgtt ccggccatgg tccctccccc
      361 ggggcctgat ccaatcgcca aatctatcct aacagcccgg cgtgcgctaa tgtcgcagga
      421 agccagggtc cgagggaagg ggaaaccaca tgtccgaccg cgtgagcagc taccgcttct
      481 ttcaggaggc ctacctcgtc aacgatctgg atcaggccat caggggttgg agcgacctgt
      541 acggcgccgg ccccttccgg acggtcatgc accacaagac cgaccgcttt cactaccgcg
      601 gcacggatca ggaagccgac gtctcctacg cgttcggcta cctgggcgac atgcagattc
      661 agttcatcga gcagcacgac gacaccccct ccatctaccg cgacatgtac ccggccggcg
      721 aggaaggctt tcaccacgtt gccatcctcg tccacgactt cgaggccgag tacgaacgcc
      781 tggtcgggaa ggggttcacc gacgcgtgcc ggctctttgc cgacggcgtg gacgccgcct
      841 acttcgatac ccggtcggtc aacggctgct tcacggagat tcatggcgac ccgccccgca
      901 atcttggccg ctgggcgcag atgcgccgcg cccacgagct ctggcggccg ggtgacgacg
      961 tctacatgac gctgtagcgc ggcggggcgg cgttttcgaa ccggccggac ggccccgcct
     1021 ttgtgggcga cgttcgcatg aacgcctgaa accggaacgg ccggttgcgc tgaaacccgg
     1081 ttcgggttaa aaccccgctc gttcggaatc cgatccgttc ttggggagat taaaatgctg
     1141 agaagagttg cggcgtctct gcgacgcccg gtggcgtcac tttgcgccat cttgtttgtg
     1201 tccggccccg ctctggcggc cgaaatcgag gagatcgtcg tcaccgcccg gggtgtcgag
     1261 cagagcgtca gggacatccc ggtggccatt tccgtggtca gcgaggagcg catggccaat
     1321 ctcaatctga agagcctgga ggacatcgcc gaggtgacgc cccagctcaa catcgtccgc
     1381 gccagctccg gcagcggcac gagcatcagc atccgcggca tcggctcctc gccgagcacc
     1441 atcggcatcg agcagtccgt gtccaccatc gtcgacggcg tttactaccc ccagggccgg
     1501 gtgatcaacg aggccatgtt cgacgtccgt caggtggcca tcctgaaagg cccccaggcg
     1561 ctgtacttcg gcaagaacgc cacggccggc gtcatctccg tggagaccaa caaccccggc
     1621 gacgagcttg agatcatcgg ccgcgtgggt tacgagttcg agcaggagcg catgagtacg
     1681 aaggcatcat ctccagcccg ctcaccgaga ccctgggcgc gaggcttgca ctgcgctata
     1741 ccgacatgaa cgagggttac atcagcaatc ccgtcggcac gaccacctac accaccctcg
     1801 accaggcatt ctctccgccg gtcgcgaccg ttcttgaaaa tccgccgccc gaccgagaca
     1861 agtggccggg caccgagagc ttttacgggc gcctgaccct ggcctgggat ccgaacgatc
     1921 agttcggact gaacatcaag ggatc
//
LOCUS       DBNHKV010000175         2815 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_520818, whole genome shotgun sequence.
ACCESSION   DBNHKV010000175 DBNHKV010000000
VERSION     DBNHKV010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_520818"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="AC16PP_05610"
     CDS             <1..916
                     /locus_tag="AC16PP_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR6481139.1"
                     /translation="FKGLLLGLFFMSVGMGIDLAEIFTRPVWIVLSVAGLFVIKALVT
                     ANVARGFGIAWRHAVEMGLLLGQGGEFAFVVVGAALALGLLPDATAQFMLIVVGITMF
                     LSPLVAKVARSAGDLLRERATDDVDEEIDLAADLGGHVIIVGYGRTGQLLASMLDRQM
                     VPHAALDLDRDRVAALRSGGAPVFLGDACRPAMLSKLRLKHAAALAICTDDPGATERI
                     LTSARSAVGRVPIIARARDNVHAAELLGLGATRVVPELLESGLQMGHVMMEEIGLPGT
                     VARDLVEALRSEAERGLSAAADSLETST"
     gene            complement(927..1328)
                     /locus_tag="AC16PP_05615"
     CDS             complement(927..1328)
                     /locus_tag="AC16PP_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481140.1"
                     /translation="MHHTFGHFNNRGTPAMHVGEGQRHDQAMIAPRGLEEETLVQMIR
                     GVEGYTALLSKNRGTMDDGCYETLSRALGGIIESLSVLTTGPRGRLDGDRLREKLEAI
                     AGQELSKRAREEAGSPRSAQAHLRLLEKTGA"
     gene            1560..1835
                     /locus_tag="AC16PP_05620"
     CDS             1560..1835
                     /locus_tag="AC16PP_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481141.1"
                     /translation="MQVTFVEIAQHSATQTVTFSEAELPGASSKPQISADLQQTTSGS
                     KPLSLLVEGWGDPPDSSGANACVPSDPMGTACGRRVASDPIVTISYH"
     gene            1991..2521
                     /locus_tag="AC16PP_05625"
     CDS             1991..2521
                     /locus_tag="AC16PP_05625"
                     /inference="COORDINATES: protein motif:HMM:NF025710.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HIR6481142.1"
                     /translation="MNNRIQNNKAFEMVIATIAALAPVSMAVAANPEPVVAEVTFVDP
                     VTITETNALQFGLLDAGLESGEAVVIAPDGTVTDADNRVVGGSQAAAELTVTASAAQA
                     ITILIDTVASGSGYTLGTFVCSYDGGADTACDGAGYSQTSVASAFLNVGATLTGDGTA
                     AAGDADGSFNVTVSYQ"
BASE COUNT          470 a          858 c          916 g          571 t
ORIGIN      
        1 gttcaagggc ttgttgctcg ggctgttctt catgtcggtg ggaatgggaa ttgatctggc
       61 cgagatcttt acccggccgg tgtggatcgt tctctccgtc gcaggccttt tcgtcatcaa
      121 agcgctggtc acggccaacg ttgcgcgcgg gttcggcatt gcctggcggc acgcggtgga
      181 gatggggctg ctgctcgggc agggcggtga attcgccttc gtggtggtcg gagcggccct
      241 ggcgctcggc ctgctgccag atgccaccgc ccagttcatg ctcatcgtgg tgggcatcac
      301 catgttcctt tcgcccctcg ttgccaaggt ggcccgaagt gccggggatc tccttcggga
      361 aagggctact gatgacgtcg acgaagagat tgatctggcg gccgacctgg gcggccatgt
      421 catcatcgtc ggctatggtc gaacggggca gctcctggca tccatgctcg atcgtcagat
      481 ggtccctcac gccgcgttgg atctggatcg ggaccgggtg gcagcactgc ggtctggggg
      541 agcgcccgtt ttcctcggtg acgcctgccg tccggcgatg ttgagcaagc tacgtttgaa
      601 gcatgcggcc gcgttggcaa tctgcacgga cgatccgggc gctacggaaa ggattctgac
      661 cagcgcccgg agcgcggtgg ggcgcgtgcc catcatcgcc cgagcccgtg acaacgttca
      721 cgccgccgag ctactgggac taggggctac ccgggtggtc cccgagctcc tcgagtccgg
      781 gctgcagatg ggccacgtca tgatggaaga gatcgggctt ccgggcaccg ttgcccggga
      841 tctcgtggag gcgctgcgaa gcgaagccga gcgtggcctg agcgctgcgg ccgattcgtt
      901 ggaaacttcg acctgaagcc gggcgctcag gcgcccgtct tctcgagcaa cctaaggtgt
      961 gcctgagcag aacgcgggga gccggcctcc tcccgggcgc gttttgacag ctcctgaccg
     1021 gcgatagcct ccagcttctc tcgcaaccgg tcaccatcga gccggccccg cggcccggtc
     1081 gtcagaacgg atagcgattc gatgatcccg ccgagggctc gagacagcgt ctcgtagcat
     1141 ccgtcgtcca tggtgccccg gtttttggat agcagcgcgg tatagccttc gaccccacgg
     1201 atcatctgca ccagggtttc ttcctcgagc cctcgcggcg cgatcatggc ctggtcgtgt
     1261 cgttgcccct cacccacatg cattgccggt gtcccccggt tgttgaagtg cccgaacgta
     1321 tggtgcacaa gaagcttgta ctagtggttg aggaatcatt gaccgattct gaagaaggcc
     1381 cctgttttcg tgcacatttc tttacaagtc ggaaggccgt cgagggtaga ctcaaacggg
     1441 tgggcgtagg tcgttggctg gaaagataat cgatggcacc tgggtatcga gttttcctct
     1501 tgctgtttct gtggttcgcg ttcggttgcg aagctctggc aggttcccgg acggtggcgg
     1561 tgcaggtcac cttcgtagaa atcgcgcagc acagcgcgac ccagaccgtg accttctccg
     1621 aagctgagct gccgggagca tcctccaagc cgcaaatctc cgccgatctt cagcagacga
     1681 cgtctggaag caagccgctc agccttctcg tcgagggctg gggcgaccct ccggattcat
     1741 ctggtgccaa cgcctgtgtg ccgagcgatc ccatgggtac tgcatgcggc cgacgggtgg
     1801 ccagcgatcc gatcgtgacg atttcctacc actgaacctt tcccttgccg ggcctcggcg
     1861 ggataaagcg tcgatctgag actggcttct cggaacccgc agcatttgcg gagcatactg
     1921 cagctgggcg ttgtcaatgc ccgtggatgc cgtgccagaa ggacctgtgc aataggacga
     1981 ggaggacgtc gtgaacaata ggatccagaa taataaagcc tttgagatgg tcatcgcgac
     2041 catagccgcc ctagcgcctg tttccatggc ggtggcggcc aacccggagc cagtggttgc
     2101 cgaggtcacc ttcgtcgatc ccgttaccat caccgagacg aacgcgctgc agtttggcct
     2161 gctcgacgcc ggcctagaga gcggcgaggc cgtcgtgatt gcgcccgatg gcaccgtgac
     2221 cgatgcagac aaccgggtcg tgggggggtc tcaggctgcg gccgagctta ccgtcacagc
     2281 gtcggccgct caggccatca ccattctcat cgacaccgtc gccagcggct cgggctacac
     2341 cctcggtacc ttcgtctgca gctacgatgg cggggcagat accgcctgcg atggcgccgg
     2401 ctactcgcaa acctctgtag ccagcgcgtt tctcaacgtc ggtgcaacgc tgacgggcga
     2461 cggaacggcg gccgccggcg acgcagatgg aagcttcaac gtcacggtct cgtaccagta
     2521 acgatcgacc tgagttgcct ggcggacaat gccgggtaac gagacttgcg gttaaggtcg
     2581 tggccgatag cggaaggaaa tggatcgccc ttctcgaaca gcccccctcg gcgcctcgtg
     2641 gcgctgggcg ttgcttgcgg ccacgctagc cgttctctgt tggcctggca cggtcctgtc
     2701 cataggatct acgctcaacg gggaggatcg aaaccttctt cttctggacc tgcgcgtgga
     2761 tggtgccgtc cgcgcggaaa gcctcgccac gctggcggac gggtcgggaa gttac
//
LOCUS       DBNHKV010000176         7782 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_96593, whole genome shotgun sequence.
ACCESSION   DBNHKV010000176 DBNHKV010000000
VERSION     DBNHKV010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7782)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_96593"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="AC16PP_05630"
     CDS             <1..374
                     /locus_tag="AC16PP_05630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6481143.1"
                     /translation="ISEALHRDLRAAGSALGASVLCPGWVDTKIAEAERNRPTAMTSA
                     KNPEGHGLPLGDTLKQGMPPEVVAEKVLNSIREERFYILPHAGWDEVVLGRVDAIVAR
                     GAPYELDREALVAKRGLGEEV"
     gene            436..1188
                     /locus_tag="AC16PP_05635"
     CDS             436..1188
                     /locus_tag="AC16PP_05635"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6481144.1"
                     /translation="MRLEGRTCVVTGGSSGIGAATVRRFVAEGAEVLIADIGVEQARG
                     LAAELGDAVSVVECDVRFEDSVRGVAAAAHARWDHVDVLVNNAGSELNRAYDEATVEE
                     WDRVQDTDLKGPWLMCKHLVPRMVERGSGSVINVSSLNGLVGFPLSTAYGGAKGGLVV
                     FTRDMAIELASSGVRFNCVCPGVIETPMMERWTDLMPDKGEAQEMLRGVMPIGRMGRA
                     DEVAGAILFFASDDSSLCQGAVLSVDGGFTAQ"
     gene            complement(1228..1452)
                     /locus_tag="AC16PP_05640"
     CDS             complement(1228..1452)
                     /locus_tag="AC16PP_05640"
                     /inference="COORDINATES: protein motif:HMM:NF016231.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HP1242/YdcH family protein"
                     /protein_id="HIR6481145.1"
                     /translation="MFEYEQDAVQALLKANDRFQDLYSQHAALKIQVHEAEIGVHPMD
                     DLTLNQLKRQKLLAKDKMAHMIQKYQRRHA"
     gene            complement(1541..1987)
                     /locus_tag="AC16PP_05645"
     CDS             complement(1541..1987)
                     /locus_tag="AC16PP_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6481146.1"
                     /translation="MIVGVHHVAIGVPDFETGLAFYRDVLGFEVLSASELSGNELADR
                     AIGLPDVRAQVAMLKAPNIYIELWSYSNPEPDDRRQRPCDFGYPHFALQVRDMQTEYA
                     RLSAAGMEFVGEPVELGGSAAIYGKDPFGNVIEIYEIRDPEIAQLS"
     gene            2098..3381
                     /locus_tag="AC16PP_05650"
     CDS             2098..3381
                     /locus_tag="AC16PP_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6481147.1"
                     /translation="METEHQTGCPYRHADEIDFMDPVVQENWFDAYDIIREESPAYFM
                     PQIGMYVLTRYDDIEHVLRRPTQFTVGPDVQSTEPLIKHPEALALYEEKGWRRYTPLG
                     ENLPKHAHYRGLVDPALTAAAIRKRESFVRATVNELIDEWIDEGEIEFIDRFAEPLPM
                     IVIAELLGFPRMDLPQLKEWSYAWVLPFSRGLSLEQEMWAVNKHIELQHYIFETMQRK
                     RKDPKDDIITRLTRIEIEDVDIGAKRPLTDTEIIGITDHLLIGGNETTTFALSNGLWL
                     LFRNPDVYRALQADRDKIKNFVEEVLRIESPTQGLYRFVTEDCEIGGVPVPKGATLSI
                     RYGAGNHDPGRFPHPDTPELGRKNAGRHLAFGIGEHVCPGATLSRFEQRWAWEILLER
                     LRNIRPAPGKNDYKHRGGMWVRALEEIYMQFDKAG"
     gene            complement(3396..4298)
                     /locus_tag="AC16PP_05655"
     CDS             complement(3396..4298)
                     /locus_tag="AC16PP_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6481148.1"
                     /translation="MTMTSGKRPDAGRYQDVFFESDDGLDLYARDYPNPDAPLTVLCL
                     HGLTRNSADFADLAAALQPDFRVLAMDQRGRGRSAYDPDPSRYALPTYVKDAFALMDH
                     LALEHVAVIGTSMGGLMAMLMAARQPDRICGIVFNDVGPVVEAEGLRRIQGYVGKGGE
                     IASWADAVALTQANNGAAFPGLSEADWLAFARRLFVENDAGVPVLAYDAKISEPMQAD
                     QTTAVPADLWPVFDAMGEIPLLVVRGALSDILSRETVEEMARRHPGTTAVEVPDRGHA
                     PMLDEPEAMQAVSEFLAALGGKRG"
     gene            4338..5648
                     /locus_tag="AC16PP_05660"
     CDS             4338..5648
                     /locus_tag="AC16PP_05660"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6481149.1"
                     /translation="MTEINRMENPVCPASLAEATLADAEVLECPYPTYDLLREQAPVW
                     QDPRTGIFVVTRYEDLRMICTDTERFSNHRPSNDHDKLTGIARKAYELFVEKGWVPGE
                     SLAARDDPEHRQMRSIFNEAFRPKRINALDSEVQALAYRLIDDFVDDGHCNFVKQFAV
                     PLPLIIICRQMGAREEDVWQIKAWTDAWFRGTGFNLTEEEVIWATEMEIQAQHYFQPI
                     FERLRKEPDGTLLSDLVNTEVPGWGRTLNDNELHAEMMQDTFVGGSETTTNALSAGIM
                     LLAQNPGVWERLKAEPEKYLRTFCEEVVRLESPVQGLTRMAKTDIELHGVTIPRGAVI
                     DARFAAGNRDPEHFDNPGEVDLDRPNAASHLGFSSGTHYCLGAPLARRELYWGFKAFL
                     DNVASFRLAPGRNDLRHYPNYALRALSELHIEFERVPSGDRTDI"
     gene            5685..>7782
                     /locus_tag="AC16PP_05665"
     CDS             5685..>7782
                     /locus_tag="AC16PP_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HIR6481150.1"
                     /translation="MRKAVMIRCLIGWCAGLLAAAVAQAQTQNIVYFPEWGVHLQPYY
                     VRHIQDSGAADQLTVINYAFVVPAPNAAGDVVCQMDDPVAAYKQLYTAAMSVDGIPDV
                     DDAGQPLRGHFNQFKKLKAEHPHLKVLIAIGGWLGSVWWSDAATPESRETFVASCIDL
                     FIRGNLPVAGTAGGEGVAAGVFDGFDIDWEYPITGGAGGTHHSADDDVNLTATLAEFR
                     RQLDAVAAELETGRLLLTIATPGSAFRGDNYQINADQAHVDWFNLMTYDFHGTWENKT
                     GHQTNILTSVNDPSSDAFKLSIDNAVRLYRDTYGVSVEKLVIGAAFYGRGWSGVSTTD
                     DGLYQSGRTAPGIYEDGYNYYRDLAPLMLGDYTLHWDPLALASWLYSPVERTFWTLDE
                     PQSIALKRRYADAFGLGGVMAWEVSGDDEDGTLVSAMRTGDPGAPVQSSSSALDGAVE
                     VTQPASCAISVEGFNQVLGAETTVSAPAKVDQVEFFGNGVSLGFDNFPPWSWAWFNLP
                     AGVHEITAVASDWAGSSTSSTSVLLNVYSEADMALWQVGASYQPGDEVFYDGCIYQAK
                     RNHVGSRVRLPTGGRYWDLVTCSDCGGGGGGGGEGGNQPPVIESITASPSTSIRANEN
                     VTLTVVAGDPEGWIASVVFAVGAGVESGVYSEFDTWTWTWSNVPAGTHEVTVTAHDNS
                     GATASDRITIVAASGSG"
BASE COUNT         1357 a         2487 c         2593 g         1345 t
ORIGIN      
        1 tcatcagcga ggcgcttcac cgggatctcc gcgcggccgg ttcggccctc ggcgcgtccg
       61 tcctctgccc ggggtgggtg gatacgaaga tcgccgaagc cgagcgcaac cggcccacgg
      121 ccatgactag cgcgaaaaac cccgaagggc atgggctgcc gctgggagat accctcaagc
      181 agggtatgcc gccggaggtg gtggcggaaa aggtgctgaa ctccatccgg gaggagcggt
      241 tctacatcct cccccacgcc ggctgggacg aggtggttct gggcagggtc gacgccatcg
      301 tcgcccgggg tgcgccttac gagctcgatc gcgaagccct ggtggccaag cggggtctcg
      361 gcgaggaagt atgagcattg ccggcctggc aggcgcgagc cgcgggccgg gggaccaggt
      421 tgaaggagag acaggatgag gcttgagggc agaacgtgcg tggtgacggg cgggtcgagc
      481 ggcatcgggg ccgccaccgt tcgccggttc gtggctgagg gggccgaggt gctgattgcc
      541 gacatcggcg tcgagcaggc gcgcgggctt gccgcggagc tcggcgatgc ggtgagcgtg
      601 gtggagtgcg acgtgcgctt cgaggacagc gtgcgcggcg tggcggccgc ggcccatgcg
      661 cggtgggacc atgtggacgt gctcgtcaac aacgcgggct cggagctcaa ccgcgcctac
      721 gacgaggcca ccgttgagga atgggaccgg gttcaggaca ccgatctgaa gggtccctgg
      781 ctcatgtgca agcacctggt cccccggatg gtggagcgcg gcagcggcag cgtgatcaac
      841 gtatcctcgc tcaacggcct ggtggggttt cctctgtcga ccgcctacgg tggggccaag
      901 ggcggtctgg tggtcttcac ccgcgacatg gccatcgagc tcgccagctc cggggtgcgt
      961 ttcaactgtg tctgcccggg cgtcatcgag acccccatga tggaacgctg gacagatctg
     1021 atgcccgaca agggagaggc tcaggaaatg ttgcggggcg tcatgcccat cggccgcatg
     1081 ggccgggcgg atgaggttgc tggagcaatt ctgttcttcg cctccgacga ctccagcctc
     1141 tgccagggcg ccgtactctc cgtggacgga ggctttaccg cccagtaggc cgcgcagatg
     1201 gacgtcggca ggccccgcgg gtcggggtca ggcgtgccgg cgttggtatt tctggatcat
     1261 gtgcgccatc ttgtccttgg cgaggagctt ctggcgcttg agttggttta gggtcagatc
     1321 atccatagga tggacgccga tctccgcctc gtggacctgg atcttcagcg cagcgtgctg
     1381 gctgtagagg tcctgaaagc ggtcgttcgc tttcagaagc gcctgcaccg catcctgttc
     1441 gtactcgaac atgcttcacc ccttcccagg ttgtgcacgt ctgaaactag tcaaaaattc
     1501 cgtggcttac aagactgcgg ggctgtttcg gcggaggtcg tcagctcagc tgggcgattt
     1561 cgggatcgcg gatctcgtag atctcgatga cgtttccgaa cgggtcctta ccgtagatgg
     1621 cggcggaccc gccgagctcc accggctcgc cgacgaattc catgccggct gccgagagcc
     1681 ttgcgtactc ggtctgcatg tcccgcacct gcaacgcgaa gtgggggtag ccgaagtcgc
     1741 acgggcgctg gcgtctgtcg tccggctccg ggttgctgta gctccacagc tcgatgtaga
     1801 tgtttggtgc tttcagcatg gcgacctggg cccgcacgtc gggcagcccg atggcccggt
     1861 cggcgagctc atttccggag agctcgcttg cgctcaagac ctcgaaaccg agcacgtccc
     1921 gataaaaggc caggcccgtc tcgaaatccg gaacgccgat ggccacgtgg tgaacgccta
     1981 cgatcattgc tatccccttg cgttgctgaa tgctgagggc cttgatggta ccgtctcttc
     2041 ggggcgcaat tgtataaatt gtatagttgg cagggtttta agggagaggg gagcatcatg
     2101 gagaccgagc atcagacggg gtgtccgtac cggcacgcgg acgagatcga tttcatggat
     2161 ccggtggtgc aggagaactg gttcgacgcc tacgacatca ttcgtgagga gtcgcccgcc
     2221 tacttcatgc cccagatcgg catgtacgtg ctgacccgct acgacgacat cgagcacgtg
     2281 ctgcgccggc cgacccagtt cacggtgggc ccggacgtgc agagcaccga accgctcatc
     2341 aagcatcccg aggcgctcgc gctatacgag gaaaaaggct ggcgtcgcta cacgccgctt
     2401 ggtgagaacc tgcccaagca cgcccactac cgtgggttgg tggaccctgc gctgacggcg
     2461 gcggccatca ggaagcgtga aagcttcgtg cgcgctaccg tcaacgagct catcgacgag
     2521 tggatcgacg agggcgagat cgagttcatc gaccggttcg ccgagccgct gccgatgatc
     2581 gtcatcgccg agctcctcgg ttttccccgc atggatctgc cccagctcaa ggagtggtcc
     2641 tacgcctggg tgctgccgtt ttcccgcggg ctgtccctcg agcaggaaat gtgggctgtg
     2701 aacaagcaca tcgagctgca gcactacatc ttcgagacca tgcagcgcaa acggaaggat
     2761 ccgaaggacg acatcatcac ccggctcacc cggatcgaga tcgaggacgt ggacatcggc
     2821 gccaagcgcc cgctcaccga cacggagatc atcggcatca ccgatcacct gctgatcggc
     2881 ggcaacgaga ccaccacgtt cgcgctttcc aacgggctgt ggctgctctt tcgcaacccg
     2941 gacgtctacc gggcattgca ggccgatcgc gacaagatca agaacttcgt cgaagaggtg
     3001 ctgcgcatcg aatcccccac tcagggcctc taccggttcg tcaccgagga ctgcgagatc
     3061 ggcggggttc ccgttcccaa gggcgctacc ctcagcatcc gctacggtgc cgggaatcac
     3121 gaccccgggc gctttccgca cccggacacg ccggagctgg gtcgcaagaa cgcgggccgg
     3181 cacctggcgt tcgggatcgg cgagcacgtc tgcccaggtg ccaccctgag ccgcttcgag
     3241 cagcgctggg cctgggagat cctgctcgag cggctgcgca acatccggcc ggcgccgggc
     3301 aagaacgact acaagcaccg cggcggcatg tgggtccgcg ccctcgagga gatctacatg
     3361 cagttcgaca aggcgggata gcgcctgtca ggacctcagc cgcgtttgcc gccgagcgcg
     3421 gcgagaaact cgctcaccgc ctgcatggct tccggttcgt ccagcatcgg cgcgtggccg
     3481 cgatcgggca cctccacagc ggtggtgccg ggatggcgcc gcgccatctc ctcgacggtt
     3541 tcccgggaga gaatgtccga gagcgcgccg cgcaccacca gcagcggaat ctcgcccatg
     3601 gcgtcgaaga cgggccagag atcggcgggt acggccgtgg tctggtccgc ctgcattggc
     3661 tcggagatct tcgcatcgta ggccagcacc ggaacgccag cgtcgttctc gacgaacagg
     3721 cgccgggcga aggcgagcca gtcggcctcg ctcaagccag ggaaagccgc gccgttgttg
     3781 gcctgggtca gcgccaccgc gtctgcccac gaggcgatct cgccgccctt acccacgtag
     3841 ccctggatgc ggcgcagacc ttcggcctcc accaccgggc caacgtcgtt gaaaacgatg
     3901 ccgcagatac ggtccggctg ccgcgcggcc atgagcatgg ccatgagccc gcccatggag
     3961 gtgccgatga cggccacgtg ctcgagcgcc aggtggtcca tcagggcaaa cgcgtccttc
     4021 acgtatgtcg gcagcgcgta acggctcggg tcgggatcgt aagcggaccg gccccggcct
     4081 cgctggtcca tggccagcac gcgaaagtcc ggctggagcg ccgcggccag atccgcaaaa
     4141 tctgccgagt tgcgggtcag cccgtgcagg cagagcacgg tcagcggcgc gtccgggttg
     4201 gggtagtcgc gggcgtaaag atccagcccg tcgtcgcttt cgaaaaacac gtcctggtag
     4261 cggcccgcgt cggggcgctt gcctgaagtc atggtcatgg cagcattagc gtggcgaaag
     4321 atgcgcatgg taacggaatg acagaaatca accgaatgga gaatccggtc tgcccggcga
     4381 gcctggccga ggccaccctg gcggacgccg aggtcctcga gtgcccctat cccacctacg
     4441 accttctgcg cgagcaggcc cccgtctggc aggacccgcg cacgggcatc ttcgtggtca
     4501 cccgctacga ggatctgcgt atgatctgca cggacaccga gcgcttcagc aaccaccggc
     4561 cgagcaacga tcacgacaag ctcaccggca tcgcgcgcaa ggcctacgag ctctttgtag
     4621 agaagggctg ggtgcccggc gaatcgctgg cggctcggga cgacccggag caccggcaga
     4681 tgcgctccat ctttaacgaa gccttccgcc ccaagcggat caacgcgctg gattcggagg
     4741 tgcaggcgct cgcctaccgt ctgatcgacg acttcgtcga cgacggccac tgcaacttcg
     4801 tcaagcagtt cgccgtgccc ctgccgctga tcatcatctg ccggcagatg ggcgcgaggg
     4861 aggaggacgt ctggcagatc aaggcgtgga cggacgcctg gttcaggggc accggcttca
     4921 acctcaccga agaggaggtg atctgggcca cggagatgga gatccaggcc cagcactact
     4981 tccagcccat tttcgagcgg ctgcgcaaag agccggacgg caccctgctg tcggatctgg
     5041 tgaacaccga ggtgcccggc tggggcagga cgctcaacga caacgagctg cacgccgaga
     5101 tgatgcagga tacgttcgtg ggcggctcgg agaccaccac caacgcgctt tccgccggca
     5161 tcatgctgct ggcccagaat ccgggcgtgt gggagcggtt gaaggcggag ccggagaagt
     5221 acctgcgcac gttctgcgag gaggtggtcc ggctggagag ccccgttcag gggctcaccc
     5281 gcatggccaa gacggacatc gagctccacg gcgtcacgat cccccgcggt gccgtgatcg
     5341 acgcccgctt tgccgccggc aatcgggacc cggagcactt cgataacccg ggcgaagtcg
     5401 acctggaccg ccctaacgcg gcttctcatc tgggcttcag ctccggcacc cactactgcc
     5461 tgggggcccc gctggcgcgc cgggagcttt actggggctt caaggccttt ctcgacaacg
     5521 tcgcctcctt ccggctcgcc cccggcagga acgacctgcg ccactacccc aactacgcgt
     5581 tgcgggcctt gagcgagctg catatcgagt tcgagcgggt tccgtccggc gaccgaactg
     5641 atatatagtc aggcatattg tctttatagt tcgaggtggg agctatgcgc aaggccgtca
     5701 tgattcggtg cctgattggc tggtgtgcgg gcttgctggc tgctgcggtt gcgcaggccc
     5761 agacgcagaa catcgtctac tttcccgagt ggggcgtcca cctgcagccc tactacgtga
     5821 ggcatattca ggattcgggt gccgccgatc agctcaccgt catcaactac gccttcgtcg
     5881 tgccggcacc caacgcggcc ggcgacgtcg tctgccagat ggacgacccg gtggcggctt
     5941 ataagcagct ctacaccgcg gcgatgagcg tcgacggcat ccccgatgtt gacgatgccg
     6001 gccaaccgtt gcgcggccac ttcaaccagt tcaaaaagct caaggccgag catccgcatc
     6061 ttaaggtgct gatcgccatc ggcgggtggc tcggctcggt gtggtggtcg gatgcggcaa
     6121 cgcccgagtc ccgcgagacg ttcgtggcgt cgtgcatcga cctcttcatc cgcggcaacc
     6181 tgccggtggc gggcaccgcg ggcggcgaag gcgtggcggc cggcgtcttc gacggcttcg
     6241 acatcgactg ggagtacccc atcaccggcg gggcgggcgg gacccaccac agcgccgacg
     6301 acgacgtgaa cctcaccgcg acccttgccg agttccgccg gcagctcgat gctgtggccg
     6361 ccgagctcga aactggccgg ctgctgctca ccatcgccac ccccggcagc gcgttccgcg
     6421 gcgacaacta ccagatcaac gccgatcagg cgcacgtgga ctggttcaac ctgatgacct
     6481 acgactttca cggcacctgg gagaacaaga cgggtcacca gaccaatatc ctgacctccg
     6541 tgaacgaccc ctcctcggat gcgttcaagc tgtcgattga caacgccgtg cgcctttacc
     6601 gcgacaccta cggcgtttcc gtggagaagc tcgtgatcgg cgctgccttc tacggccgcg
     6661 gctggagcgg ggtaagcacg acggacgacg gcctttacca gagcggacgg acggcgcccg
     6721 gcatctacga ggacggctac aactactacc gcgacctggc accactgatg ctcggtgact
     6781 acaccctgca ctgggatccg ctggcgctgg cctcctggct ttacagcccc gtggagcgga
     6841 ccttctggac cctggacgag cctcagtcga ttgcgctcaa gcggcgctac gcggatgcct
     6901 tcgggcttgg tggggtgatg gcctgggaag tgagcggcga tgacgaagat gggaccctgg
     6961 taagcgcgat gcgaaccggt gaccccgggg ctccagtcca atcttcgagt tctgccttgg
     7021 acggggccgt cgaagtgacg cagccggcga gttgcgccat ctccgtggag gggttcaatc
     7081 aggtcctcgg cgctgagacg accgtgtcgg cgcccgccaa ggtggatcag gtggagttct
     7141 tcggcaacgg ggtttccctg ggttttgaca attttccgcc ctggagctgg gcctggttca
     7201 acctgcccgc cggggttcac gagattacgg cggtcgcctc ggactgggcg ggtagctcga
     7261 ccagttcgac ctcggtatta ctgaacgtct actccgaagc agacatggcg ctgtggcagg
     7321 tgggcgccag ctaccagccg ggcgacgagg tgttctacga cggctgcatc taccaggcca
     7381 agcgcaacca cgtgggctcc cgggtgcgcc tgcccacggg cgggcgctac tgggatctgg
     7441 tgacctgcag cgactgcggc ggtggcggcg gtggcggcgg tgaaggcggc aatcagcctc
     7501 ccgtgatcga gagcatcacg gcttctccat ccacgtcgat cagggcgaac gagaacgtga
     7561 cgctcacggt tgtggcgggc gatcccgaag gctggattgc cagcgtcgtc ttcgccgttg
     7621 gggctggggt cgagtctggt gtctattccg agttcgatac ctggacctgg acctggtcca
     7681 acgtccccgc cggcactcac gaggtcaccg tcaccgccca cgacaactca ggcgccaccg
     7741 ccagcgacag aatcaccatc gtcgcggcga gcggctccgg ct
//
LOCUS       DBNHKV010000177         2243 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1865815, whole genome shotgun sequence.
ACCESSION   DBNHKV010000177 DBNHKV010000000
VERSION     DBNHKV010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1865815"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="AC16PP_05670"
     CDS             complement(<1..822)
                     /locus_tag="AC16PP_05670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR6481151.1"
                     /translation="MAQAYDYIIVGAGSAGCVLAYRLSADPGVNVLLLEAGGVDRSPL
                     IGIPKGIGKLVRSPDHAWFFPVEQPRQPDLPASEVWVRGKVVGGSSSINGMIYSRGHP
                     EDYEEWMALGGPAWGWAEMKAAYRSIEDHELGSADHRGAGGLVHVSTGKLRYPAAEAM
                     IEAGREMGLERKDDLNDEMLEGVGYYAHNIRRGRRQSAARTFLKAARRRPNLHVQSQA
                     HVDRVCFDGNRAVAVACRVAGDRRVFEARGEIILSAGTILSPKILQLSGIGPADHL"
     gene            complement(824..1414)
                     /locus_tag="AC16PP_05675"
     CDS             complement(824..1414)
                     /locus_tag="AC16PP_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR6481152.1"
                     /translation="MSKTVEIFFDFLSPPSYLAWTQVPGIVQRTGASAVWRPMFTIGL
                     HQLTENRSPVMVPNKARWIAGDLQRFARRYGVTLNPNPHGIINILPADRAAALAEKEG
                     AVEQLMATAYPAMWVDGRDLSDPEVLGEVIAAAGLDASRYLAAIETDEVKNLLKQNTQ
                     EAADRGAFGAPTFFVGDEMFFGQDRLDFVEEALAGG"
     gene            complement(1436..2167)
                     /locus_tag="AC16PP_05680"
     CDS             complement(1436..2167)
                     /locus_tag="AC16PP_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003210139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6481153.1"
                     /translation="MTTSEKVVLVIGAGVATGGAVARRFAREGFTACMARRQGEKLQG
                     LVDEIAAAGGRAHGYSCDATEEAQVVDLVERVEAEVGPIEVACYNAAVGASHSITEFP
                     ADAYERVWRINTFGAFVMGREVSRRMVERGRGTILFTGATSALRGKARLAAFAGSKHA
                     LRALSESMARELFPQNIHVAHVIIDGPIDTPLIRRVMPSVFEERPADGVLLPDDIAET
                     YWNIHCQPRSAWLHETQLRPWLEPW"
BASE COUNT          384 a          784 c          712 g          363 t
ORIGIN      
        1 caggtggtcg gccgggccga tgccggagag ctgcagaatc ttgggagaga ggatggtgcc
       61 ggccgagagg atgatctcgc cccgggcctc aaagacccgc ctgtcacccg cgacgcgaca
      121 cgccaccgcc acggcccgat tgccatcgaa gcacacccga tcgacgtggg cctgcgactg
      181 cacgtgcagg ttggggcgcc gccgggcggc cttcagaaaa gtgcgcgccg cgctctgccg
      241 acgtccgcgg cggatgttgt gagcatagta gccaacaccc tcgagcatct cgtcgttgag
      301 gtcgtccttg cgctccaggc ccatttcgcg gccggcctcg atcatggcct cggctgcggg
      361 atagcgcagc ttgcccgtgc tgacgtgcac cagcccaccc gctccgcggt ggtcggctga
      421 ccccagctcg tgatcctcga tggagcgata agccgccttc atctccgccc agccccacgc
      481 cgggccgcca agcgccatcc actcctcgta gtcctcggga tgcccgcggg agtagatcat
      541 gccgttgatg gagctggaac cgccgaccac cttgccccgc acccacacct cggaggccgg
      601 caggtccggc tgccgcggct gctcgaccgg aaagaaccag gcgtggtcgg ggctcctgac
      661 cagcttcccg atacccttgg gtatgccgat gagggggctc ctgtccaccc cacccgcctc
      721 caggagcaga acgttcacgc cgggatctgc ggagagccga taggccagca cgcagccggc
      781 cgaaccagca ccgacgatga tgtagtcgta cgcctgggcc atctcagccc cccgccagcg
      841 cttcttcgac gaaatccagg cgatcctggc cgaagaacat ctcgtcgccc acgaagaagg
      901 tcggagcgcc gaaggcaccc cgatcggcag cttcctgggt gttctgcttg agcaggttct
      961 tcacctcgtc ggtctcgatg gcggcaaggt agcgggatgc atcgagaccg gcggcagcga
     1021 tgacctcgcc gagcacctcg gggtccgaca ggtcgcggcc gtccacccac atggccgggt
     1081 acgccgtggc catgagctgc tccactgcgc cctctttctc cgccagcgca gcggcacggt
     1141 cggccggcag gatgttgatg atgccatggg gattgggatt caaggtgacg ccgtaacggc
     1201 gcgcaaatcg ctgcaggtcg ccggctatcc agcgtgcctt gttcggcacc atgaccggcg
     1261 aacggttctc cgtaagctgg tgcaggccga tggtaaacat gggtcgccag acggcgctgg
     1321 cgccggtccg ctggacgatg cccggcacct gggtccacgc cagatagctc ggcgggctta
     1381 agaagtcgaa gaaaatttcc acggttttgc tcacgatgac accacctcca accgtctacc
     1441 agggctccag ccaggggcgc agctgggttt cgtgaagcca tgcgctgcgg ggctggcagt
     1501 gaatgttcca atacgtctcg gcgatgtcgt cgggaagcag aacgccgtcc gcaggacgct
     1561 cctcgaaaac cgacggcatc acgcggcgga tgagcggcgt gtcgatgggc ccgtcgatga
     1621 tcacgtgggc cacgtggatg ttctgcggga aaagctcccg ggccatgctc tcgctcaatg
     1681 cgcgaagcgc gtgcttcgac cctgcaaagg ccgcgagccg ggctttcccg cgcagcgcgg
     1741 aggtggcgcc tgtgaagagg atggtcccac gcccccgctc caccatgcgc cgggacacct
     1801 cccgccccat gacgaaggca ccgaaggtgt tgatgcgcca gacgcgctcg taggcatctg
     1861 ccgggaactc ggtgatgctg tgggatgcac ccacggcggc gttgtagcag gccacctcga
     1921 tgggtccgac ctcggcttcc acccgctcga ccagatccac gacctgggct tcttcggtcg
     1981 catcgcagct gtaaccatgg gcccgtccgc ccgccgcggc gatctcgtcc accagcccct
     2041 gcagcttttc tccctgacgt cgcgccatgc aggcggtgaa accctcccgg gcaaaacgcc
     2101 gcgccaccgc gccgccggtg gcgaccccgg ccccgatgac gagcaccacc ttctccgaag
     2161 ttgtcatagg caatccccct tggcccgaca gtaacccacc cggattcccg aatatatcgc
     2221 gagccgccgg tcaggagttc aga
//
LOCUS       DBNHKV010000178         7727 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_521271, whole genome shotgun sequence.
ACCESSION   DBNHKV010000178 DBNHKV010000000
VERSION     DBNHKV010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7727)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7727
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_521271"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="AC16PP_05685"
     CDS             complement(<1..322)
                     /locus_tag="AC16PP_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012593.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylate symporter family
                     transporter"
                     /protein_id="HIR6481154.1"
                     /translation="MNLTIRILLGMGLGLVLGSSIQAAEFDPSHWVHVYLVGGLIDAG
                     GQIFVRSLQLMVVPLVFVSLVCGAASLSATDSIGRVAGKTVALYLFTTAVAISIALLL
                     ATIVQ"
     gene            complement(339..506)
                     /locus_tag="AC16PP_05690"
     CDS             complement(339..506)
                     /locus_tag="AC16PP_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0043448 - alkane catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HIR6481155.1"
                     /translation="MRKWQCIVCGWIYDEAEGYEEEGIAPGTAWEDVPEDFVCPECGV
                     SKEDFEMIEVG"
     gene            666..1574
                     /gene="ubiA"
                     /locus_tag="AC16PP_05695"
     CDS             666..1574
                     /gene="ubiA"
                     /locus_tag="AC16PP_05695"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HIR6481156.1"
                     /translation="MEKTRQPPLSAVIAYARLMRLDRPVGTLLLLWPTLAALWVAAEG
                     LPPVDLIVVFAVGTFLMRAAGCVVNDYADRNLDPGVARTANRPLATGAVSTNAALGAF
                     FVLAFLALTLLLFLNTLTRWFAVAGLAIAVAYPFMKRWTYLPQVVLGAAFSWGIVMAF
                     TAVRHVVPEPGWLLFLASVLWIVAYDTMYAMVDREDDLRVGIKSTAILFGEADRLMIG
                     LLQTGTILTLVLVGQRLGYHSAYFWGIAVAAGLFCYQQYLIRDRSREGCFRAFANNVW
                     VGFSLFVGVAWETMTRGAFQPGGGLS"
     gene            1628..2089
                     /locus_tag="AC16PP_05700"
     CDS             1628..2089
                     /locus_tag="AC16PP_05700"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR6481157.1"
                     /translation="MAWLTALMVVLMVTVVVLRYAFDQGAIPIQEAIVYLHATVIMLG
                     IPCALRDGVHVRVDILHSRLSERARGIIDVAGHLLFLVPTCLLILYFSIPYARNAWLV
                     LEGSPEVGGIPAVFLLKTLIPVMAVALLLQGLAETVRRLPIILPSHFGSER"
     gene            2094..3497
                     /locus_tag="AC16PP_05705"
     CDS             2094..3497
                     /locus_tag="AC16PP_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR6481158.1"
                     /translation="MVELIPIFMFLAVFAALLAGYPVALTLAGVALLFALGGMAGGTF
                     DGRDLGFLPGRLFGIITNPTLIAVPLFVFMGVMMEKTRIAENLLSSLSRLMAGTPGGL
                     AFAVIVVGMLMAASTGIVGASVVTLGLMSLPTMLRDRYDPAFASGTISATGTLGQIIP
                     PSIALVLLGEVLSNAYQQAQLNQGIFAPDTVSVGDLFAGAVVPGLILVGLYLAYTMLV
                     AFLRPGTAPAASSVADAVPDAVPDAVPDGSGWQLLGGLLPPMLLIVAVLGSILAGYAT
                     PTEAAGVGAAGATILAAAYRALDVGVLRRVCRSTFDTTAMVFFILVGASLFSLVFRGY
                     GGDDLVHSWFNDMPGGTWGALAIVMLVIFLLGFMLDFIEITFVVVPIVGPVLLAMGVD
                     PIWLGVLIAVNLQTSFLTPPFGFALFYLRGVAPPTVSTADIYRGVAPFVLLQILLLCL
                     LFIWPSLATWLPERIYR"
     gene            3494..4678
                     /locus_tag="AC16PP_05710"
     CDS             3494..4678
                     /locus_tag="AC16PP_05710"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6481159.1"
                     /translation="MSERPTTITVSRVWDGEALCDADAVRIENGRIAAVGDARELSKG
                     AVAVHCPGATALPGLIDAHVHLELNPDNHKPPESTSPDEVPRMLERAAAMVRAGITTA
                     RDLGGGAWLELGIRDKTAAGQAIGPRLLCSGQPITCPGGHCHFWGGEAGSAEEALSVL
                     ERQVTQGVDLIKVMATGGRMTNGSDPLAAQFDLETLSEITAAARAHGLPVAAHCHGTT
                     GIELAARAGVSTIEHCSWAGAEGWASDFQEPVANVILERGVWVSPTVNRGWQRMLDSR
                     EGRVLGRVRAAYRAMLELGIPFIASTDAGIPGVFHHHLPLALKVFALIAELSPQAALR
                     TATSSAARALGVHGMTGRLVPGLAADILVVDGNPLDDLDALANPVGVWARGRRVVDPH
                     GA"
     gene            complement(4719..5558)
                     /locus_tag="AC16PP_05715"
     CDS             complement(4719..5558)
                     /locus_tag="AC16PP_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6481160.1"
                     /translation="MELKATEYSVASGIATVRLNRPHRLNAWTGRMHSEYRWCIDRAE
                     RDPAVRAIVVTGNGRGFCVGGDAEALEGHARKGGYDPGTPARLAAPGYGRAPEFDASF
                     AYHYGLSKPVIAAVNGPAAGVGLALACFADLRFAAAGVKLTTAHGKLNLPAEYGLSWL
                     LPRMIGLTRANDLLLSSRVFLAEEALAMGLVNAVYPQAELMDEVYAYTRELIRTVSPA
                     SLAQTRWQIYRDLHRSVAESVRASEQLLDEMMRQEDYREGVAAFLEKRAPSWSGSAGE
                     TGE"
     gene            complement(5592..6677)
                     /locus_tag="AC16PP_05720"
     CDS             complement(5592..6677)
                     /locus_tag="AC16PP_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR6481161.1"
                     /translation="MMVMRILALVLIFGLAGCGAPQQTAPEAVPDPDKVYRWKLITTW
                     PKNLPALGMAPERLAENLRVMSRGRLDIRVYGAGELVGAFEVFDTVRQGTAEMGHGAA
                     YYWRGKLPVAAMFATVPFGMMAQEMNGWLRYGGGMELWREAYEPFGLVPLAAGNSGVQ
                     MAGWFNKEINSLEDLQGLKMRIPGLGGEVLKRAGGVPVSMAGSEIYTAMQTGVIDATE
                     WVGPYNDLAMGLQDVARYYYYPGWHEPGPTLEAIVNKAAWDSLPEDLQRMVEVAAAAA
                     NEDLLSEFTARNASALKTLLSKHNVELRRLPDDVLAKLKVESEAVVVEATGDSDLARR
                     IRESYGRFLSDVREYHAISEQAYINAR"
     gene            complement(6695..>7727)
                     /locus_tag="AC16PP_05725"
     CDS             complement(6695..>7727)
                     /locus_tag="AC16PP_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024754.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR6481162.1"
                     /translation="RAEIKNALAYMRLTVDRHSDTAFERVVNTPARGIGDRTVEAIRA
                     QARARNVSLWQACKEALAENVLASRTAAKIADFLGLIDGLAEQTAELDLHEVADACIQ
                     SSGLLEYHMRERGERGLARKENLEELVSACRQFSGEQVFPLLADAAEQTSILEEFLDQ
                     VALDAGDRQAEAGPAVQMMTLHSAKGLEFPMVFLAGMEEGLFPHRMSAEEPGRMEEER
                     RLCYVGITRAKQTLYLTHAETRRLHGTDTYNRPSRFLLEIPTELVHEVRMGGAVQRPY
                     GRPAEAGHAVGAEGGLRMGQRVLHAKFGEGVVLQAEGSGERARVQVNFAGAGAKWLML
                     GYANLQALD"
BASE COUNT         1191 a         2673 c         2443 g         1420 t
ORIGIN      
        1 gctgaacgat cgttgcaagc agcagggcga tggaaatggc cacggccgtg gtgaacagat
       61 aaagggcaac ggtctttccg gccacccgac cgatgctgtc cgtcgccgac aggctggcag
      121 caccgcacac caacgacacg aataccaggg gaaccaccat gagctggagg ctgcgaacga
      181 atatctggcc gccagcgtct atcaggccgc cgacgaggta gacgtggacc caatgggaag
      241 gatcgaattc cgccgcctgg atcgaagagc cgaggacgag ccccaagccc atgccgagta
      301 gaatgcggat cgtaaggttc acgacgttct aatcggcctc agccgacctc gatcatctcg
      361 aagtcttctt tgctgacgcc gcactccggg caaacgaaat cctcgggcac atcctcccaa
      421 gccgtacccg gcgcaatgcc ctcttcctca taaccctcgg cttcgtcgta gatccagccg
      481 cagacgatgc attgccattt tcgcattctt ggtcgcttcc ctttgttcaa gccggcgccg
      541 gcgcaaaggc gcgataaact actcatcccc cgcggaaaaa tcaatcgtgc ggccggcggc
      601 cgcctggcct gcctggtggc gaaacgctac actccgccgc caagctcaag cccgcccgcg
      661 cgaacatgga gaaaacccgt cagccgccgc tctccgccgt gatcgcgtac gcgcgcctga
      721 tgcgcctcga ccggcccgtg ggcaccctgc tgctcctgtg gccgaccctg gccgctctct
      781 gggtagccgc cgaggggctg ccgcccgtgg atctcatcgt ggtctttgcc gtcggcacgt
      841 ttctgatgcg ggccgccggc tgcgtggtga atgactacgc cgatcgtaac ctcgatcccg
      901 gcgtcgccag aaccgctaat cgaccccttg cgaccggagc cgtcagcacc aacgccgcgc
      961 tgggcgcctt cttcgtgctg gccttcctcg ccctgaccct gctgctcttc ctcaacacgc
     1021 tgacccgatg gttcgccgtc gcaggcctgg ccatcgcggt ggcctatccc ttcatgaagc
     1081 gatggaccta ccttccgcag gtggtgctgg gcgccgcgtt cagctggggc atcgtcatgg
     1141 cgtttaccgc ggtacggcac gtggtgccgg agcccggctg gctgcttttc ctggcaagcg
     1201 ttctctggat cgtcgcctac gacaccatgt acgccatggt ggaccgggag gacgatctgc
     1261 gagtgggaat caagtccacg gccatactgt ttggcgaggc cgaccgtctg atgatcgggc
     1321 ttctgcagac cggcacgatc ctcaccctgg tcctggtcgg ccagcggctc ggctaccaca
     1381 gcgcctactt ctggggcatc gccgtcgcgg ccggcttgtt ctgctatcag caatacctga
     1441 tccgggaccg aagccgggag ggttgcttcc gcgccttcgc caacaacgtg tgggtcggtt
     1501 tttcgctgtt cgtcggcgtc gcatgggaaa ccatgacccg cggcgccttc cagccaggtg
     1561 gggggctgag ctgaacacac ggctgctcga gcacgccgat cgcgtcaccg aggcgacggc
     1621 ccgcagcgtg gcctggctaa ccgccctcat ggtcgtgctg atggttaccg tcgtcgttct
     1681 gcgctacgcg ttcgaccagg gcgcgatacc gattcaggaa gccatcgtct atctgcacgc
     1741 cacggtgatc atgctcggca ttccctgcgc cttgagagac ggcgtccacg tcagggtgga
     1801 catccttcac tcgcgcttga gcgaacgcgc ccgggggatc atcgacgttg ccgggcacct
     1861 gctgttcctc gtgcctacct gcctgctaat cctctatttc agcattccct atgcccgcaa
     1921 cgcgtggctc gtgctcgagg gatcacccga ggtcggcggc attccggccg tctttctcct
     1981 gaaaacgctc attccggtca tggcggttgc cctgctgctg cagggcctcg cggagaccgt
     2041 gcgccggctg cccatcattc tgccctccca tttcggtagc gagcgctgac aacgtggtcg
     2101 agctcatacc gatcttcatg ttcctggccg tcttcgccgc cctgctggcc ggataccccg
     2161 tagcgctgac cctggccggg gttgccctgc tgttcgccct gggcgggatg gccggcggaa
     2221 ccttcgacgg ccgtgatctg ggtttcctgc ccggccggct gttcggcatc atcacgaacc
     2281 ccacgctcat cgcggtgccg ctgttcgtct tcatgggcgt gatgatggag aagacgcgga
     2341 tcgccgagaa cctgctcagc agcctttcgc ggctgatggc gggaacgccc ggggggctgg
     2401 cttttgccgt catcgtggtc ggcatgctca tggctgcgag caccggtatc gtcggcgcct
     2461 cggtggtcac cctcgggctg atgtctctac ccaccatgct tcgcgatcgc tacgacccgg
     2521 ccttcgctag cggcaccatc agcgcgacgg gaacgctggg ccagatcatt cctccgtcca
     2581 ttgccctggt gctgctgggc gaggttctct cgaacgccta tcagcaagcg cagttgaatc
     2641 agggcatctt cgccccggac acggtgtcgg tcggggacct gtttgcaggc gcggtggtgc
     2701 ccggactcat cctggtcggg ctctatctgg cctacaccat gctggtcgcc ttcctgcggc
     2761 cgggcaccgc gcccgccgcc agctcggtcg cggacgcggt cccggacgcg gttccggacg
     2821 cggtcccgga cggctcaggg tggcagctct tgggcgggct gcttcctccc atgctgctga
     2881 tcgttgccgt gctggggtcc atcctcgcgg gctacgcaac cccgacggag gccgccggtg
     2941 tcggcgcagc cggagcgacc atcctggcgg cggcttatcg cgcgctggac gtcggcgtct
     3001 tgcgccgggt gtgccggtcg acgtttgaca ccacggccat ggttttcttc atcctggtcg
     3061 gcgcctcctt gttttctctg gtcttcaggg gatacggcgg cgacgaccta gttcacagct
     3121 ggttcaacga tatgccgggc ggcacctggg gagcgctcgc catcgtgatg ctggtgatct
     3181 tcctgctcgg cttcatgctc gacttcatcg aaatcacctt cgtcgtggtg cccatcgtcg
     3241 gccccgtgct tctggccatg ggtgtagacc cgatctggct gggggtgctg atcgcggtca
     3301 atctccagac ctcgttcctg acgccaccgt tcggcttcgc gctcttctat ctgcgcggcg
     3361 tggctccccc cacggtttcc acagccgaca tctaccgcgg cgtggccccg ttcgtgttgc
     3421 tgcagatcct gctcctgtgc ctgctcttca tctggcccag ccttgcgacc tggttaccgg
     3481 agcgcatcta ccggtgagcg agcggccaac caccattacg gtgagccgcg tatgggacgg
     3541 ggaggctctt tgcgacgccg atgccgttcg catcgaaaat ggacgcatcg cggcggtcgg
     3601 cgacgcccgg gagctgagca aaggcgccgt ggccgtccac tgccccgggg ccaccgcgct
     3661 ccctgggctc atcgacgccc acgtccacct ggagctcaac cccgacaatc acaagcctcc
     3721 cgagtcgacg agcccggacg aggtgccgag aatgttggaa cgggccgcgg ccatggtccg
     3781 ggcaggaatt accaccgcgc gcgacctcgg cggcggtgcc tggctcgagc tcggcatccg
     3841 ggacaaaacg gcggccggcc aggctatcgg gccccgactg ctctgctccg ggcaacccat
     3901 cacgtgtccc ggcggtcact gccacttctg gggcggggaa gcgggctccg ccgaagaagc
     3961 cctgtccgtg ctggagcgtc aggtgaccca gggcgtggac ctgatcaaag tcatggccac
     4021 gggcggccgg atgactaacg gatccgatcc gctggccgcc cagttcgacc tggagacgct
     4081 gagcgagata acggcggccg ccagggcgca cgggctgccc gtcgccgccc actgccacgg
     4141 caccacgggc atcgagttgg ccgcccgcgc cggcgtgtcc accatcgagc actgctcctg
     4201 ggccggtgcg gaaggttggg catcggactt ccaggagccg gtggcaaacg tgatcctcga
     4261 gcgcggcgtc tgggtgtcgc ccaccgtcaa tcgtggctgg cagcgcatgc tggacagccg
     4321 cgaagggcgg gtgctcggcc gcgttcgtgc cgcctaccgg gcgatgctgg agctgggaat
     4381 tcccttcatc gcttctacgg acgccggaat tcccggggtg tttcatcacc acctgccact
     4441 ggcattgaag gtcttcgccc tgattgccga gctcagcccc caggccgctt tgcgaaccgc
     4501 gacgtcatcc gcagctcgag ccctgggcgt tcacgggatg acggggcgcc tcgtgccggg
     4561 tcttgccgcc gacattctgg tggtggatgg aaatcctctc gacgacctcg acgccctcgc
     4621 aaacccggtg ggggtctggg cccgcggccg gagggttgtc gacccgcatg gcgcctgagc
     4681 aacggggcgc ctgggtcgaa cggccccgag ccaaacgatt actcgccagt ctctccagct
     4741 gatccggacc agctgggggc gcgtttctcc agaaacgccg ccaccccttc gcgatagtct
     4801 tcctgccgca tcatctcatc gagcagctgc tcagaggctc tgacggactc ggcaacgctt
     4861 cgatgcaggt cgcggtatat ctgccagcgg gtctgggcca gggacgccgg ggacaccgtg
     4921 cggatgagct ccctggtgta agcgtagacc tcgtccatga gctccgcctg ggggtacacg
     4981 gcgttgacga gccccatggc gagggcttcc tcggcgagga agacacggct cgacagcagc
     5041 aggtcgttcg cccgggtgag gccgatcatc cgtggcaaca gccaggacag cccgtactcc
     5101 gcggggaggt tcagcttgcc gtgggcggtg gtgagcttga cgcccgcagc ggcgaaacgc
     5161 aggtctgcga agcaggcgag cgccagaccg acccccgcgg ccgggccgtt cacggctgcg
     5221 atcacgggtt tggaaagccc gtagtgatag gcaaagctgg cgtcgaattc gggcgcccga
     5281 ccgtatcccg gcgctgcgag ccgggcaggc gttcccggat cgtagccgcc cttgcgcgcg
     5341 tgcccttcca gcgcttcggc gtctcccccg acgcagaaac ccctcccgtt gcccgtgacg
     5401 acaatcgcgc ggaccgctgg gtcacgctcc gcgcgatcga tgcaccagcg gtactcgctg
     5461 tgcattcgac ccgtccacgc gttgaggcga tgaggccggt tcaggcgcac ggtggcgatg
     5521 ccggacgcga ccgagtactc cgtcgccttc agctccaacg cgacctcccc gccggccggt
     5581 cgcgcagagg ctcagcgggc attgatgtag gcctgctcgc tgatggcgtg gtactcgcgc
     5641 acgtcgctga gaaaccgccc gtaggattca cgaatacgac gggcaagatc ggagtcaccg
     5701 gtagcctcga ccaccaccgc ctcggactcg actttaagct ttgccagcac gtcgtccggc
     5761 aagcgcctga gctccacgtt gtgcttcgaa agaagcgttt tgagggcgct ggcattgcgg
     5821 gcggtgaact cgctcaggag atcttcgttg gcggcagccg cggcgacctc caccattctc
     5881 tgcagatcct cgggcaacga atcccacgcc gctttgttga cgatggcttc cagggtgggg
     5941 ccgggctcgt gccagcccgg gtagtagtag tagcgtgcca cgtcctgaag ccccatggcc
     6001 aggtcgttgt aggggcccac ccattccgtg gcgtcgatga cgcccgtctg catggccgtg
     6061 taaatctccg accccgccat ggacaccggc acgcccccgg cacgcttgag cacctcgccg
     6121 ccgagccccg ggatgcgcat cttcaatccc tgaagatcct ccagggagtt gatttccttg
     6181 ttgaaccagc ccgccatctg cacgccgctg ttaccggcgg cgaggggcac gaggccgaac
     6241 ggttcgtagg cctcgcgcca cagctccatg ccgccgccgt agcgcagcca gccgttcatc
     6301 tcctgggcca tcatgccgaa aggaaccgtg gcgaacatgg cggctaccgg gagcttgccg
     6361 cgccagtagt aggcggctcc atggcccatc tcggccgtgc cctggcgtac cgtatcgaac
     6421 acctcgaacg cgccgaccag ctccccggcg ccgtagaccc ggatgtctag ccgaccccga
     6481 ctcatgaccc gcagattttc ggccaggcgc tccggcgcca tgccgagcgc cggaaggttt
     6541 ttcggccagg tcgtaatgag cttccagcga tagaccttat cgggatcggg aaccgcttcg
     6601 ggcgccgtct gctgcggcgc gccgcagccg gcgaggccaa atatgagcac cagggccagg
     6661 atccgcatca ccatcatgtc gctcttctcc agtttcaatc gagggcctgc aggtttgcat
     6721 agcccagcat caaccatttc gcgccggcgc cggcgaagtt gacctgcacc cgggcgcgct
     6781 caccggaacc ctcggcctgg agcaccacgc cctcgccgaa cttggcgtgt agcacccgct
     6841 gacccattcg caggccgccc tcagccccga ctgcgtgccc ggcttctgca ggccggccgt
     6901 agggtcgctg caccgcgccg cccatgcgca cctcgtgaac gagctccgtc ggaatctcca
     6961 gcaggaagcg cgacggccgg ttgtaagtat ccgttccgtg caggcgccga gtctccgcat
     7021 gggtgaggta gagcgtctgc ttggcgcggg tgatgcctac gtagcacagc cgcctttcct
     7081 cttccatgcg cccgggctct tcggcggaca tgcggtgggg gaagaggcct tcctccatcc
     7141 ccgcgagaaa taccatgggg aactccagcc ccttggccga gtgcagggtc atcatctgca
     7201 ccgcaggacc cgcttccgcc tggcgatcac ccgcgtccag agctacctgg tcgagaaact
     7261 cctcgagtat cgacgtctgc tcggcggcgt cggccaggag agggaatacc tgctcgccgc
     7321 tgaactggcg gcaggcgctg accagctcct ccaggttctc cttgcgagcc aggccacgct
     7381 ccccccgctc gcgcatgtgg tactccagaa ggccgctgga ctgaatgcag gcgtcggcga
     7441 cctcgtgcag atccagctcg gccgtctgct ccgccagccc gtctatgagc ccgaggaaat
     7501 cggcgatctt ggccgccgtc cggctggcaa gcacattctc ggcaagggct tccttgcacg
     7561 cctgccacag ggacacgttg cgggcgcgcg cctgggcccg gatcgcctcc acggtcctgt
     7621 ccccgatacc gcgagccggg gtgttcacaa cccgctcgaa ggccgtgtcc gaatgccggt
     7681 ccacggtcag acgcatgtag gcaagcgcgt tcttgatctc cgccctt
//
LOCUS       DBNHKV010000179         2458 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_166728, whole genome shotgun sequence.
ACCESSION   DBNHKV010000179 DBNHKV010000000
VERSION     DBNHKV010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_166728"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="AC16PP_05730"
     CDS             complement(<1..716)
                     /locus_tag="AC16PP_05730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6481163.1"
                     /translation="MTLDLFYRVLQEFALIYPLFMAYLWMVGGIYYLFHWERAGHARV
                     DRPPPLPEYPGVSVLVPAHNESFAIVETIRSLQNLKYPEYEIIVINDGSTDDTAEILD
                     ELAEEGGIRVIHLASNQGKAAALRVGAMASNHEFLVCIDGDAILDCHAVHWIMRHFLI
                     GPRVGAVTGNPRVRNRSTLLGKIQVAEFSSIIGLIKRAQRIYGRLFTVSGVVSAFRKT
                     ALYRVGFWDLTKLTDDIDIS"
     gene            complement(721..>2458)
                     /gene="pgaB"
                     /locus_tag="AC16PP_05735"
     CDS             complement(721..>2458)
                     /gene="pgaB"
                     /locus_tag="AC16PP_05735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HIR6481164.1"
                     /translation="GFEPITLDQWAAAAEGEPLPPRPVLLTFDDGYASFAEHVMPVLK
                     LFDFPAVLAPVTSWVDAPEGSVVQYGDQPMPRERFLTWDQLREISKTGLVEIATHTHD
                     MHRGLTGNQFGNELPAAVTRRWNDERNAYEPEADYEARIRSDLKRSRDRIAEQLGKPP
                     RAVVWPYGAYNLQAASVARTLGLTYTLTLDSLPNDPRIDGGRIHRELVSTDTSMLFFT
                     TWALGKEQETPLRAAHVDMDYVYDPDPAQAERNLGRLLDRIKAMKLSFVFLQAFADPD
                     GDGVADALYFRNRHLPMRADLFNRVAWQLKTRAGVEVFAWMPVMAFELPDAERNQALA
                     VKAYDGSHESRYHRLSPYNPEARRIVGEIYDDLGRGSRFLGVLYHDDGFLTDREDVSP
                     AALARTVPDEAARGDGVLAPAVVSDFRGKTRFLIDFTHELSDVLRRHQPALLTARNLY
                     ARPVVDPQAEAWFAQSLGAFQRSYDYTAVMAMPYLEQAEEPEAWLSDLVEKVRAIPGG
                     MERTVFELQTVDWREGNPVRAATLANQMDLLLDRGVKHIAYYPDDFIQGYPPVPLVRS
                     RLSVNYHPALEE"
BASE COUNT          451 a          785 c          797 g          425 t
ORIGIN      
        1 cagctgatgt cgatgtcgtc ggtgagcttg gtcaggtccc agaacccgac gcgatagaga
       61 gccgtctttc gaaacgccga aaccacgccc gagactgtga acagacgccc gtagatgcgc
      121 tgggcccgct tgatcagccc gatgatggac gagaactcgg ccacctgaat cttacccagc
      181 agcgtggagc ggttcctgac ccgcgggttg ccggtcaccg cccctactct gggaccaatc
      241 agaaagtgcc gcatgatcca gtggacggca tggcaatcga gaatggcgtc cccgtcgatg
      301 cagaccagaa actcgtgatt cgaggccatg gcacccaccc gcagcgcggc cgccttgccc
      361 tggttggagg caagatgaat gacccggatg cctccctcct ccgccagctc gtcgagaatc
      421 tcggcagtgt cgtcggtgct gccgtcgttg atgacgataa tctcgtattc gggatacttg
      481 aggttctgca gcgaccggat ggtctcgacg atggcgaacg actcgttgtg cgcgggcacc
      541 agaacggaaa caccggggta ctcgggaagc ggcggcggac ggtccacgcg ggcgtgcccg
      601 gcacgctccc agtgaaagag gtagtagata ccgcccacca tccacagata ggccatgaac
      661 agcgggtaga tgagcgcaaa ctcctggaga actcgataga aaagatccag cgtcacgcca
      721 ctactcctcc agcgccgggt ggtagttgac ggacaggcgc gagcgcacga ggggcaccgg
      781 cgggtagccc tggatgaagt cgtcgggata gtaggcgatg tgcttcacgc cgcggtccag
      841 cagaagatcc atctggttcg ccagcgtcgc cgcccgcacg gggttgccct cgcgccagtc
      901 gacggtctgg agctcgaaca ccgtccgctc catgccgccg gggatcgccc gcaccttctc
      961 caccaggtcg gaaagccacg cctccggctc ctccgcctgc tcgagatagg gcatggccat
     1021 gacggccgtg tagtcgtagc tacgctgaaa agcgccgagg ctttgagcaa accaggcctc
     1081 ggcctgggga tccacgaccg gcctcgcgta caggtttcgc gcggtgagca gcgcgggctg
     1141 gtgccgacgc agcacgtccg aaagctcgtg agtgaaatcg atgaggaatc gggttttgcc
     1201 gcggaagtcg ctcacgaccg ccggcgccag aacgccgtcg cctctcgctg cctcgtcggg
     1261 gacggtgcgg gcgagagcgg ccgggctgac gtcctcgcga tcggtcagga acccatcatc
     1321 gtggtagagc acgcccagaa agcggctgcc gcgaccgaga tcgtcataga tctcccccac
     1381 gatgcgccgg gcctccgggt tgtacggaga cagccggtgg tagcgggact cgtgggaccc
     1441 gtcataggcc ttcaccgcaa gcgcttgatt gcgttcggca tcaggcagct cgaacgccat
     1501 gaccggcatc caggcaaaca cttccacgcc cgccctggtt ttcagctgcc aggccacccg
     1561 gttgaaaaga tcggcacgca tgggcaggtg cctgttgcgg aagtacagcg cgtccgcgac
     1621 gccgtcgccg tcgggatctg cgaaggcctg caggaagaca aaggacaact tcatcgcctt
     1681 gatacgatcc aggagccggc cgagatttcg ttcggcctgg gccgggtccg ggtcgtacac
     1741 gtaatccatg tccacgtgcg ctgcccgcag cggtgtctcc tgctcctttc ccagggccca
     1801 cgtggtgaaa aacagcattg acgtgtcggt gctcaccagc tcgcgatgaa tgcggccgcc
     1861 atcgatgcgc ggatcgttcg gcaggctgtc cagcgtcagc gtgtacgtga gcccgagggt
     1921 ccgggcgacg cttgccgcct gaaggttgta ggctccgtag ggccacacca cggcccgagg
     1981 cggcttgccg agttgctcgg cgatgcggtc ccggctgcgc ttgaggtcac ttctgatgcg
     2041 cgcctcgtaa tcggcctccg gctcgtaggc gttcctctcg tcgttccacc ttcgagtcac
     2101 agcagcaggc aactcgttgc cgaactggtt gcccgtgagc ccacgatgca tgtcgtgggt
     2161 atgcgttgcg atctccacga ggccggtctt cgatatctct ctgagctgat cccacgtcag
     2221 aaatcgctcc cgcggcatgg gctggtcgcc gtactggacg acgctacctt cgggggcgtc
     2281 cacccagctg gtcaccggcg cgagcacggc cggaaaatcg aaaagtttca gaacgggcat
     2341 cacgtgctct gcaaagctcg cgtagccgtc gtcgaaggtg agaagcaccg gccggggcgg
     2401 gagcggctct ccctcggctg cagccgccca ctggtcgagg gtgatgggct cgaagccg
//
LOCUS       DBNHKV010000180         2983 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_591884, whole genome shotgun sequence.
ACCESSION   DBNHKV010000180 DBNHKV010000000
VERSION     DBNHKV010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_591884"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="AC16PP_05740"
     CDS             <1..685
                     /locus_tag="AC16PP_05740"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIR6481165.1"
                     /translation="SDWINRTTDEIFAGRTVVVFSLPGAFTPTCSSSHVPRFNQLAGA
                     FARHGVDEIVCISVNDAFVMNEWQESQKAWDVTFLPDGNGEFTGKMGMLVRKDDLGFG
                     ARSWRYSMLVRDGVIEKMFIEPEIPGDPYEVSDADTMLDYLAPNEAHPASVTLFSRKG
                     CAHCVRAKAMLEDHGYEYEELVLNRDYTDRTLRAVAGATSLPQVFIDGRLVGGADDLE
                     AVLAEDRAA"
     gene            complement(796..1347)
                     /locus_tag="AC16PP_05745"
     CDS             complement(796..1347)
                     /locus_tag="AC16PP_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6481166.1"
                     /translation="MSNEEYSPKLSLAHATLSVTRFEEALDFYCDVLGFQITNSGEVP
                     GGGKMAFISQDPNNHHQIAMVEAAEPGSHAFVMADHLAFRTGSLQDLRILRERLTAAG
                     IETILPICHGNAWSLYFSDPEGNGLECFVDTPFHVAQPFAQGFDLDQPDEEIAAATKA
                     LIESEPEFQTMANWKAAFENRLG"
     gene            complement(1344..2882)
                     /locus_tag="AC16PP_05750"
     CDS             complement(1344..2882)
                     /locus_tag="AC16PP_05750"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase B"
                     /protein_id="HIR6481167.1"
                     /translation="MRASSDRHGYTRRAAWFGCCMALSFASAALGSTQTGPNIVLILA
                     DDLGWNDVGYHGSEISTPNIDALATQGVVLNQFHSQPTCTPTRAALMTGKSPQRMGIY
                     RQFAKISTEGLPVGEKTLADHLRAAGYQTWLTGKWHLGHSRAEFHPNARGFDHFYGHV
                     TGGIGYWDHVHGGGLDWQRNGRTLREEGYSTHLIAEEAVRLVRTRQPSRPFFLYASFN
                     APHLPNEAPEKAMQAYAGIENPQRRVHAAMVTELDAAVGEIVRTLEDEKLLDDTLIWF
                     MSDNGGLNRDAGLGGFLTLAEALDDWFEAPVPIRFLEFIRTNALDGGSDNGPLRFGKG
                     SVYEGGVRVPSFVTFPPALDPHRSDAMVTVQDVLPTLLAAAALEPADGLDGGNRWPEL
                     RQEPGESPPADYVTIGQNGAQAYYRWPWKLIATNGQHELYNLETDPLEEQNLADVETE
                     VVSTLALELSQLPRAESLHVPVYRVVWDPDFFGGEEDRQPWADIIDRAMHEQRAVKTG
                     GYLP"
     gene            complement(2866..>2983)
                     /locus_tag="AC16PP_05755"
     CDS             complement(2866..>2983)
                     /locus_tag="AC16PP_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6481168.1"
                     /translation="FGQPEELGDACAYLCSAQAGFICGQNLQLDGGSYAGLI"
BASE COUNT          501 a          923 c          977 g          582 t
ORIGIN      
        1 atctgactgg atcaatcgca ccacagacga gatctttgcc ggccggacgg tggtcgtctt
       61 ttcgctgccc ggcgcgttca cgccgacctg ctcatcgtcc catgtgccgc gtttcaacca
      121 gctcgccggg gctttcgcac gccacggcgt ggacgagatc gtctgcattt cggtcaacga
      181 cgcgttcgtc atgaacgaat ggcaggagag ccagaaggcc tgggacgtga cgtttctgcc
      241 cgatggcaat ggcgagttca ccggaaagat gggaatgctg gtccgtaagg acgatcttgg
      301 tttcggcgcg cgctcgtggc gctactcgat gctcgtccgc gacggggtga tcgagaagat
      361 gttcatcgag cccgaaatcc cgggtgaccc ttatgaagtg tccgacgccg acacgatgct
      421 cgactacctt gcgccgaacg aggcgcatcc ggcaagcgtg acgctgtttt cacgcaaggg
      481 ctgcgctcac tgcgtacggg cgaaggcaat gctcgaggat cacggttacg agtacgagga
      541 gctcgttctc aaccgtgatt acacggaccg gacgctgcgt gccgtggcgg gcgccacgtc
      601 gttaccgcag gtcttcatcg acggccgcct ggtcgggggg gccgatgatc tggaggccgt
      661 tctggcggag gatcgggcgg cctagggacc gggcctggca gggcgctcat gcagacgtgc
      721 ctcttccggg cgcgtggcct gagcgcccag ccatcgccaa cccgcctcgt tcgatcacca
      781 gcccctcgcg atggttcaac ccaggcggtt ctcgaacgct gccttccagt tggccatggt
      841 ctggaactcg ggttccgact cgatgagcgc cttcgtcgcg gcggcgatct cctcgtcggg
      901 ttgatccaga tcaaagccct gcgcgaaagg ttgggcgaca tggaaaggcg tgtcgacgaa
      961 gcattcgaga ccgttgccct ccggatcgct gaagtacaaa gaccacgcgt tcccgtgaca
     1021 gatcggcagt atggtctcga taccggcagc cgtgagccgc tcgcgcagaa ttcgcaggtc
     1081 ctgcagggag ccggttcgga aagcgaggtg gtcggccatg acgaaggcat ggctgccggg
     1141 ctcggcggcc tccaccatgg cgatctggtg atggttgttg ggatcctggc tgatgaaagc
     1201 catctttccc ccgcccggga cctcaccgct attggtgatc tgaaaaccca gcacgtcgca
     1261 ataaaagtcg agcgcctctt cgaatctggt gacgctcaac gtggcatggg ccagcgagag
     1321 tttcggtgag tattcttcgt tgctcatggc aggtagcctc ctgtttttac agctctttgt
     1381 tcatgcatcg cgcggtcgat gatgtccgcc cagggttgcc gatcctcctc tccgccgaag
     1441 aagtcggggt cccagaccac ccggtaaacg gggacgtgca ggctctcggc tctcggcagc
     1501 tggctgagct cgagggcgag ggtgctcacg acctcggtct cgacatcggc caggttctgc
     1561 tcttccagcg gatccgtttc caggttgtag agctcgtgct ggccgttcgt cgcgatcagc
     1621 ttccacggcc accggtagta ggcctgggca ccgttctggc cgatggtgac gtagtcggcg
     1681 ggcggtgact cgccgggttc ttgccgcaac tcgggccagc ggttcccgcc gtccagaccg
     1741 tctgcgggct cgagggctgc ggctgccaga agcgtcggca gcacgtcctg caccgtcacc
     1801 atggcgtcgg aacggtgggg atcaagtgct ggcgggaagg tgacgaagga gggcacgcgc
     1861 acgccacctt cgtagacgga acccttgccg aaacgcagcg gcccgttgtc ggatcctccg
     1921 tctaaagcat tggtgcgtat gaactcgaga aaccgaatgg gcaccggcgc ttcgaaccag
     1981 tcatccaggg cttcggccag ggtgaggaag ccccccaacc ccgcgtcgcg attcaggccg
     2041 ccgttgtcac tcatgaacca gatcagggtg tcgtcgagca acttctcgtc ctcgagcgtg
     2101 cggacaatct ctccaacggc ggcatccagc tcggtgacca tggctgcgtg cacgcgtcgt
     2161 tgcggatttt caatgcccgc gtaggcctgc atggctttct cgggggcctc gttcggcagg
     2221 tgaggtgcgt tgaagctcgc atatagaaag aacggtcggc tcggctgcct cgtgcgcacc
     2281 agacgtacgg cctcctcggc gatcaggtgc gtgctgtatc cctcctcacg caaggttctg
     2341 ccgttgcgct gccaatccag cccgccgccg tgcacgtggt cccagtagcc gatgccgccc
     2401 gtgacgtggc cgtagaagtg gtcgaagccg cgggcgttcg ggtgaaattc cgcccggctg
     2461 tgccccagat gccactttcc ggtgagccag gtctggtagc cggccgctcg gaggtgatct
     2521 gccagcgtct tctcgccgac cgggagacct tcggtgctga ttttcgcgaa ctgccggtag
     2581 atgcccatcc tttggggcga cttgccggtc atgagcgcgg cccgcgtggg cgtgcaggtg
     2641 ggctggctgt gaaactgatt cagcaccacg ccctgcgtgg cgagggcgtc gatgttcggt
     2701 gtcgagattt cgctgccgtg gtagccgaca tcgttccacc ccaggtcgtc ggccaggatc
     2761 aggacgatgt ttggccccgt ttgggtcgag cccagagcag ccgaggcgaa cgacaaagcc
     2821 atgcagcagc cgaaccacgc tgcccggcgc gtgtagccgt gacggtcaga tgaggcccgc
     2881 ataggaaccg ccgtccagct gtagattctg gccgcagatg aacccggcct gtgcgctgca
     2941 cagataggcg caggcatccc cgagctcttc cggctgaccg aag
//
LOCUS       DBNHKV010000181         3692 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1727146, whole genome shotgun sequence.
ACCESSION   DBNHKV010000181 DBNHKV010000000
VERSION     DBNHKV010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1727146"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="AC16PP_05760"
     CDS             complement(<1..825)
                     /locus_tag="AC16PP_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6481169.1"
                     /translation="MDYGIALATTMDSWRAARRAEELGFSHAWFYDTQLLNPDVFMGM
                     TLAAVNTSRIRLGTGVLIPSNRIEPVTANALVSLNKLAPGRIDFGVGTGFTGRRTMGL
                     KAIPLERMKTYIERVQALVKGETVTWDFEGETHDIAFLNPELGLINVDDPIALHVSAF
                     GPRSRDLTAELGAGWLNFGVSEPVAFGSLAKMRESWARAGRDAGDLYSTLFSLGCVLK
                     PGEAFDSPRAKAQAGTAVAVFLHDMAETSEPGSLEPVMGKAVSHVVEAYREIYLGYP"
     gene            complement(839..2044)
                     /locus_tag="AC16PP_05765"
     CDS             complement(839..2044)
                     /locus_tag="AC16PP_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR6481170.1"
                     /translation="MNTLLIVGAVFLIGLAADVAGRLTPLPRVTLLLLAGVLVGPSVF
                     ALIPSGFIDQWFPTLTHMALAMVGFLMGHQLSVAELRKRGKVVSLLALGKVVGAALVV
                     FVAVWALTGDLALALVLGGVATATAPAATFDVVHEAHARGAFADNLVSIVAIDDAWGL
                     VLFSVLLALAASMANGDALDASIVGAGVMEVLGSLVLGVVIGVPMAYLTGRLTFGERQ
                     GEPIQAESFGFVLACAGGAVLLDLSPILASMAMGSVVASLARHHARPFHAIEGVEWPF
                     MILFFVLAGASLEVEGVSLVGWLALAYLVARALGTHLGIYLTAGIIGTDGPTRRWMGL
                     ALMPQAGVALGMALIASQRLPGHAETILTLVLSTTILLELVSPIITRRILERVGAGGE
                     LPDATDTTN"
     gene            complement(2041..3321)
                     /locus_tag="AC16PP_05770"
     CDS             complement(2041..3321)
                     /locus_tag="AC16PP_05770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6481171.1"
                     /translation="MQTALQLSDSAGVRYATGMAMYFAQGIPKGLLHIAMPAWLASRG
                     VAPGEIATYLAVIVLPWAFKLVTGPLMDRYEFLPMGRRRPWVMFAQLGMVLSLLGLMI
                     VEDPVEQMGTLILLGLVVNSFTATQDVAVDGMSIDFVPAREQGRLNAFMSFGKAIGWA
                     LTSAVSGVMLVTWGLGVTAVAAAAGASVFFVALMFVREQPGERLLPWTAGQAASVHVE
                     GKTFAAVFAGVNRVLWQRGSLIVLAIMFFHGLWNGYGDALMPIAAINLFGYTTPQWSQ
                     LVAVMGLVGAGLALAAGPLIDRFGAKRMLVLTISLVAVHAFLVAQTQHLWQDTTYVRV
                     MLSIWVTMDPVIMVCVIALAMAICSRENSATQFAIYMSAANLGASLGAKAYGLVAEQS
                     TYVENYTLLGVFILVMLFTVVFHRHPLNRQSEPA"
     gene            complement(3411..>3692)
                     /locus_tag="AC16PP_05775"
     CDS             complement(3411..>3692)
                     /locus_tag="AC16PP_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HIR6481172.1"
                     /translation="DTWTWTWSNVPAGTHEVTVTAHDNSGATASDRITIVAASGSGCT
                     APAWDATLTYDKGDRVQHNDILWQAKRTVAEVEPGTSPSKWTNLGPCTS"
BASE COUNT          690 a         1226 c         1248 g          528 t
ORIGIN      
        1 cgggtagccg agatagatct cccgataagc ctccaccacg tggctcacgg ccttgcccat
       61 gacgggctct aaagaacccg gctcggaggt ctccgccatg tcgtggagga acacggccac
      121 cgcggtgccc gcctgggcct tggcccttgg cgagtcgaac gcctcgccgg gcttcagcac
      181 gcagcccagc gaaaacagcg tgctgtagag atcgccggca tcccgccccg cgcgggccca
      241 ggattcgcgc atcttcgcca gcgacccgaa cgccaccggc tcgctcacgc cgaagttcag
      301 ccacccggcg ccaagctctg ccgtcaggtc acgggagcgc gggccgaagg ctgagacgtg
      361 cagcgcgatg ggatcgtcca cgttgatgag gccgagctcg ggattgagaa aggcgatgtc
      421 gtgggtttcg ccctcgaagt cccaggtcac cgtctcgcct ttgacaagcg cctgaacgcg
      481 ctcgatgtac gtcttcatgc gctcgagcgg gatcgccttc aggcccatgg tgcgccggcc
      541 ggtgaagccg gtgccaacgc caaagtcgat gcgcccgggg gcaagcttgt tgaggctcac
      601 gagcgcgttg gcggtgaccg gctcgatgcg gttgctgggt atcagcaccc cggtgccgag
      661 gcggatgcgc gaggtgttca ccgccgccag cgtcatgccc atgaagacgt ccgggttcaa
      721 gagctgggtg tcgtagaacc aggcgtggct gaagccgagc tcctccgcgc gccgggccgc
      781 ccgccaggag tccatggtgg tcgctagagc aattccgtag tccatgtttg cgtccgtttc
      841 agtttgtcgt atcggttgcg tccggcagct cgccacccgc accgacgcgt tccaggatcc
      901 ggcgcgtgat gatcggcgag accagctcca gcaggatggt ggtgctcagc accagggtga
      961 gaatggtctc ggcatggccg ggaaggcgct gagaggcgat gagcgccatg ccgagcgcca
     1021 ccccggcctg gggcatgagg gccagaccca tccagcggcg ggtcgggccg tccgtgccga
     1081 tgatgcctgc ggtaaggtag ataccgaggt gcgtgcccaa cgcgcgggcc accaggtacg
     1141 cgagggcgag ccagccaacc aggctgacgc cttccacctc cagcgaggcg ccggccagca
     1201 cgaaaaaaag gatcatgaaa ggccattcca ccccctcgat ggcgtgaaaa gggcgcgcgt
     1261 ggtgcctggc gaggctcgcc accacgctgc ccatggccat ggaggcaagg atgggcgaaa
     1321 ggtcgagcag caccgcgccc cccgcgcagg ccagcacgaa cccgaaagat tccgcctgaa
     1381 tgggctcgcc ctgccgctcg ccgaaagtca gccgcccggt gaggtaggcc atgggcacgc
     1441 cgatgacgac gccgaggacc aggctcccca gcacctccat caccccggcg ccaacaatgg
     1501 aggcatccag cgcgtcgcca ttggccatgc tggccgccag cgccagcagc acggagaaca
     1561 ggacgaggcc ccaggcatcg tcgatggcga cgatgctcac gaggttgtcg gcaaacgctc
     1621 ctctggcatg agcctcgtgc accacgtcga aggtggcggc aggcgcggtg gccgtcgcca
     1681 ccccgccgag caccagggct agggcaaggt ctccggtcag ggcccacacg gcgacgaaca
     1741 ccaccagcgc ggcccccacc accttgccca gcgccagcag cgagaccacc ttgccgcgct
     1801 tgcgcagctc tgcgacggat agctgatggc ccatgagaaa gcccaccatg gccagggcca
     1861 tgtgggtgag cgtgggaaac cactgatcta tgaagcccga tgggatcaga gcaaatacgc
     1921 tgggtccgac gagaacgccc gccagcagca gcagcgtcac ccgcggcagc ggcgtcaggc
     1981 gcccggccac gtcggccgcc agcccgatca ggaacacggc gccgacgatg agcagggtgt
     2041 tcacgcgggc tcgctctgcc ggttcagcgg gtgccgatgg aacaccacgg tgaagagcat
     2101 gaccaggatg aacacgccaa ggagcgtgta gttctccacg taggtggact gctccgccac
     2161 caggccgtag gccttggcac ccagcgaggc gccgaggttg gccgcggaca tgtagatggc
     2221 gaactgggtg gccgagttct cccgggagca gatggccatg gcgagcgcga tcacgcagac
     2281 catgatgacc gggtccatgg tcacccagat ggagagcatc acgcgcacgt aggtggtgtc
     2341 ctgccacagg tgctgggtct gcgccacgag gaaggcgtga acggccacca gcgagatggt
     2401 cagcaccagc atgcgcttgg cgccgaagcg gtcgatgagg ggcccggccg ccagcgccaa
     2461 acccgcaccc accaggccca tgacggccac gagctgggac cactggggcg tggtgtagcc
     2521 aaagaggttg atggccgcga tgggcatgag cgcatcgccg tagccgttcc agagcccgtg
     2581 aaagaacatg atggccagaa cgatgaggct cccccgctgc catagcaccc ggttcacacc
     2641 agcaaagacg gcagcgaacg tcttgccctc cacgtgcacg gaggccgcct gaccggccgt
     2701 ccagggaagc agacgctcgc cgggctgctc ccggacgaac atcagggcga cgaagaagac
     2761 cgacgcgccg gccgcggccg cgaccgcggt caccccgagg ccccaggtca ccagcattac
     2821 cccggaaacg gccgaggtca gcgcccagcc gatggctttg ccgaagctca tgaacgcgtt
     2881 cagtcgcccc tgttcccggg cgggaacgaa gtcgatggac atgccgtcca cggccacgtc
     2941 ctgggtcgcc gtgaacgagt tgacgaccag gccgagcagg atgagcgtgc ccatctgctc
     3001 aacgggatcc tcgacgatca tgagcccgag caggctcagc accatgccga gctgcgcaaa
     3061 catcacccag ggccggcggc ggcccatggg cagaaactcg tagcgatcca tgagagggcc
     3121 ggtgaccagc ttgaacgccc agggcagcac gatcacggca aggtaggtgg cgatctcgcc
     3181 gggggcgacg ccacggctgg cgagccaggc cggcatggcg atgtgcagca gccccttggg
     3241 aatgccctgg gcaaaataca tggccatgcc ggtggcgtaa cgcacaccgg cgctgtcaga
     3301 cagctgcaga gcagtttgca cgatgctttt gagccctcac cccgaagacg cgatgatacc
     3361 ccgtccgccg ctattttaga gcgctttgag cctatctcct gcgcgcccga tcaggacgtg
     3421 cagggcccca gattcgtcca cttggacggg ctggtgcccg gctcgacctc ggcaacggtg
     3481 cgcttggctt gccagaggat gtcgttgtgc tgaacccggt cgcccttgtc gtaagtcagc
     3541 gttgcgtccc atgccggcgc cgtgcagccg gagccgctcg ccgcgacgat ggtgattctg
     3601 tcgctggcgg tggcgcctga gttgtcgtgg gcggtgacgg tgacctcgtg agtgccggcg
     3661 gggacgttgg accaggtcca ggtccaggta tc
//
LOCUS       DBNHKV010000182         7717 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1018186, whole genome shotgun sequence.
ACCESSION   DBNHKV010000182 DBNHKV010000000
VERSION     DBNHKV010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1018186"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="AC16PP_05780"
     CDS             <1..777
                     /locus_tag="AC16PP_05780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6481173.1"
                     /translation="HVTPFTYYQNTPILGRMLAEWSEMPFGALYLLPLWHPVVLAEQI
                     ATLASIAPGRFIMQCGLGDDRQGKAMGVDMSRRVGMFRASLEVMRALWRGESVDEERY
                     WHLCGARIAPLSPEPVEVWIGALVQPAIRRAAQLGEGWLASPNLTPAQTADAANVYRQ
                     ACAEFGRSPAAVALRRDIFVGATSQAARATVTPYVEAGYRGMSEDALLFGSPGEVADQ
                     LRSFADQGYTDVIVRNLSARQDESLATIERLAEVKALLTG"
     gene            815..1504
                     /locus_tag="AC16PP_05785"
     CDS             815..1504
                     /locus_tag="AC16PP_05785"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6481174.1"
                     /translation="MYRLHGATSSPFVRKTWIFMKEKGLEFEHCELDPLDKSQRFLAM
                     NPLGRIPVLEEPDGNFIADSSVICDYLERCHPGPALYPSNNRDRARALWLEEYADTAL
                     NQVCARIFWMHIIIPFRTGRPADQGEIDAFKEEAYPGVFDYLDRAAPETGDGMVGGRF
                     GIADMALAAPVRLLDLAGDPLDATRWPRFDAHYQRVVARPSAQSIVAKDLATTEVWRT
                     TGNAPASTQSG"
     gene            complement(1501..2325)
                     /locus_tag="AC16PP_05790"
     CDS             complement(1501..2325)
                     /locus_tag="AC16PP_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481175.1"
                     /translation="MLGLVLLNGIGVCSMAQADEKPVPRDAGLEFVYDDGFTESERRK
                     LEAWIAEVAGALEVLVGPLPMDVRFRFARHEGAGPVPWANTLRGRRQGVRLYVDPTQP
                     VEAFRNDWTAPHELSHLVLPFVGREHAWFSEGFASFMQFRVMRIMDVLDDEAMAARYS
                     ERLDTAAAAYSQPNRRFVEAVPQLRAEGLYPTIYWGGASYFLEMEERLKRKGASLVGV
                     LAAYLACCRRNEDDLEDLVARLDDLAGSTTFSDGLTGFRRVVGFPPHRHLQALAPR"
     gene            complement(2349..4019)
                     /gene="betA"
                     /locus_tag="AC16PP_05795"
     CDS             complement(2349..4019)
                     /gene="betA"
                     /locus_tag="AC16PP_05795"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF002550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HIR6481176.1"
                     /translation="MTGQGTGSLEVFDYIIAGAGSAGCVLANRLSEDGARSVLLLEAG
                     GSDRHLFVRMPSAFSLPIGRPRFDWCYQAEAEPALDGRSLACPRGRVLGGSSSINGMV
                     YVRGHPRDFERWESLGATGWGYADVLPYFKRAEHVLGAAEGDPYRGRAGPLTVSRGRR
                     ANPLYETFLQAAEQAGHALSDDLNGYRQEGFGDLEMTVRDGVRCSAALAYLAPVVSRS
                     NLTVRSGSPVQRLNVESGRCEGLTYRHRGATHTAVAREEVVLAAGAIGSPQLLMLSGI
                     GPGEALEPHGIPVRHELPGVGANLMDHLEIYFQQACTQPVSLNRWLNPLGKGVIGARW
                     LLTASGPGASNHFETGGFVRSSARVPWPDVQFHFLPAAVSYDGRSVADADGFQVHVGP
                     MLSPSRGRLTLRSADPDVAPVISFNYMSCEEDWQVFRRAIRLSRELFAQRAFDPYRGV
                     EIAPGSGADSDGAIDAFVRRSTESAYHPCGTCRMGNDPMAVVDSECRVRGIQGLRVVD
                     ASIFPHITNGNLNAPTIMTAEKAADAILGRRLAAEALGYFDAPASPTR"
     gene            4128..5228
                     /locus_tag="AC16PP_05800"
     CDS             4128..5228
                     /locus_tag="AC16PP_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013394.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylethanolamine N-methyltransferase
                     family domain-containing protein"
                     /protein_id="HIR6481177.1"
                     /translation="MSGSLLAGWFAPLAVYLAILILHLALPARRVDGYALDPGTGQPL
                     TYRLNGLVVFALVTSVWLAAGWKGWISWDWLYVNRWSAMGGACLVGLAYSLITVLGAP
                     PTGRSLLADLYLGRPENPQHFNKRVDAKMFLYLAGAVLLGLNTLSATAFHAGALGSAS
                     NPGVYLHAALLTFFVLDYLVFERVHLYTYDLFAERVGLKLGWGCLAFYPFFYPVGLWG
                     TAHLGKPSVIADLGWIWLALSALVFLSGWVLARGANLQKYVFKRFPERAFLGRFPPRV
                     IHGGNLALLCGGFWGVARHVNYLGEILMATGLAMSLGHLDSPWPWLYPLYYLALLVPR
                     ERDDDRRCAEKYGTLWDEYRRRVPYRIVPRIY"
     gene            5252..5746
                     /locus_tag="AC16PP_05805"
     CDS             5252..5746
                     /locus_tag="AC16PP_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013981.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HIR6481178.1"
                     /translation="MNLDLILALKVLALALSANGAPVIGKRLFGARWAIPLDGGLTFL
                     DGRPLLGSSKTVRGLVLSLATTTLVATALGYGWQLGVIYSALTMSGDALSSFVKRRRG
                     IPPSGRFRGLDQIPETALPMWVCSSALGLTGWDIAVLVALFFAGDFVLSKVLYRIGIR
                     DEPY"
     gene            complement(5740..6705)
                     /locus_tag="AC16PP_05810"
     CDS             complement(5740..6705)
                     /locus_tag="AC16PP_05810"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6481179.1"
                     /translation="MRDSEILQRLEKRLGTAHARERLGVETAHESRLFSHGIRFFHPE
                     NWYSVHSIIRNSLKVSGLYARASRNALDHRVVENHVVLDALPAGLDGLRVLHLSDLHV
                     DVSDELLHAIIERVRELTYDVCVLTGDYRFRTYGAIDDALRGMARLRSNLDGSVYAVF
                     GNHDSVTMLPALEDMGIRMLMNESHAHRHGDDHLYLAGIDDAHFFGVHDIEKALAGVP
                     DTVPRILLSHTPEVYRQAARAGVQLLLCGHTHGGQICLPGVYPVTLDSRVPRRLGRGC
                     WDYKGMRGYTSPGAGASMVAVRLNCPPELTVHVFHSAGPGVAQLQ"
     gene            complement(6750..7550)
                     /locus_tag="AC16PP_05815"
     CDS             complement(6750..7550)
                     /locus_tag="AC16PP_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6481180.1"
                     /translation="MSDSEIVLCEIDDGTATLILNRPEVLNAMNDELMGGICRALERV
                     VEDESVRVVILTGAGRGFCAGADLARAAEGPADPDLQDAPDSGDLFNGAVSALMSCPV
                     PTIARVNGPAAGGGLGLALACDVAIAAESAIFVATFGPNLGIVPDLGSTWSIPMRVGR
                     ARALGMTLLGDRITANQAEAWGLIWRSVPDEELDAEVGRVAMVLKRSSPSACTRIREC
                     VDAAIHHTFGDQLGLEMAHQGVLIPRNMQEGARAFMEKRPPEFGGDRG"
BASE COUNT         1309 a         2604 c         2538 g         1266 t
ORIGIN      
        1 cacgtcacgc cgttcacgta ctatcagaac acgccgatcc tgggacgcat gctggccgag
       61 tggagcgaga tgccgttcgg cgcgctctac ctgctgcccc tgtggcatcc cgtcgtgctg
      121 gcagagcaga tcgccaccct ggcgagcatc gcgcccgggc gcttcatcat gcagtgcggg
      181 ctgggcgatg accggcaggg caaggccatg ggggtggaca tgtcccgacg ggtgggcatg
      241 tttcgtgcga gcctcgaagt catgcgcgcc ctttggcggg gcgagagcgt cgacgaggaa
      301 cgctactggc acctctgcgg cgcccgcatc gccccgctgt cgccggagcc cgtggaggtc
      361 tggatcggcg ctctggtgca gcctgccata cggcgcgccg cccagcttgg cgaaggatgg
      421 ctggcctcgc caaacctcac ccccgcccag accgctgacg cggcaaacgt ctaccggcag
      481 gcctgcgcgg agttcggcag atcgcctgcc gccgtcgccc tgcgccgaga catattcgtc
      541 ggcgcgacca gccaggccgc gcgcgccacc gtcacccctt acgtggaggc cggctatcgg
      601 ggcatgagcg aggacgccct gctgttcggc tctcctggcg aggtggccga tcagctgagg
      661 tcctttgccg atcagggata caccgacgtc atcgtgcgca acctcagcgc ccggcaggac
      721 gaaagcctgg ccaccatcga gcgtctggcc gaggtgaagg ctctgctgac cggctgacgc
      781 ccgaagagca cgaccgaccc gagaggacgt ttcaatgtac agacttcacg gcgccaccag
      841 ctcaccgttc gtgcgcaaga cgtggatctt catgaaggaa aaagggctcg agttcgagca
      901 ttgcgagcta gaccctctgg acaagtccca acgctttcta gccatgaacc ctctgggccg
      961 gatcccggtg ctggaggagc ccgatgggaa cttcatcgcc gactcgtccg tgatctgcga
     1021 ctacctggag cggtgccatc ccgggcccgc gctctacccc tcgaacaacc gcgaccgcgc
     1081 gcgggcgctg tggctcgagg agtatgccga cacggcgctg aaccaggtgt gcgcgcggat
     1141 tttctggatg cacatcatca ttcctttccg taccggcagg ccggcagacc agggcgagat
     1201 cgacgctttc aaggaagagg cttacccggg ggtgttcgac tatctggacc gggcagcgcc
     1261 ggagacgggc gacgggatgg tgggcggccg gttcggcatc gccgacatgg cgctggccgc
     1321 ccccgtgcgg ctgctcgatc tcgccggtga cccgctggat gccacgcgct ggccccgttt
     1381 cgacgcgcac tatcagcgcg tcgttgcgcg gccttcggcg caatccatcg tcgccaagga
     1441 tctggccacc accgaggtgt ggcggaccac cggcaacgcg cccgcctcca cgcagagcgg
     1501 ctaacgggga gcgagcgcct ggagatgccg gtggggtgga aaccccacga cacggcgaaa
     1561 gccggtcagc ccgtcggaga acgtcgtcga gcccgccagg tcatcgagcc tggccaccag
     1621 gtcctcgaga tcgtcctcgt tgcgccggca acaggccaga taggccgcaa gcaccccgac
     1681 gagcgaggcg cccttgcgct tcaagcgctc ctccatctca agaaagtagc tggcgccacc
     1741 ccagtaaatg gtcgggtaga gcccctcggc acgcagctgg ggaaccgcct cgacgaaccg
     1801 gcggttcggc tggctgtagg cggcggcagc cgtgtcgaga cgttccgagt agcgcgcagc
     1861 catcgcctcg tcgtccagca cgtccatgat ccgcatgacg cggaactgca tgaagctggc
     1921 gaagccctcg gaaaaccagg catgctcgcg gccgacgaaa ggcagaacca gatggctgag
     1981 ctcgtgaggc gcggtccagt cattgcgaaa cgcctcgacc ggctgtgtcg ggtccacata
     2041 caagcgcacg ccctgacggc ggccgcgcag ggtgttggcc cagggaacgg ggcccgcccc
     2101 ctcgtgacgg gcaaatcgaa aacgcacgtc catgggcaaa ggcccgacga gaacctccag
     2161 cgcaccggcc acctcggcaa tccaggcttc cagcttgcgg cgctccgatt ccgtaaagcc
     2221 gtcgtcgtag acaaactcga gacccgcatc ccggggcacc ggcttctcat ccgcctgggc
     2281 catgctgcag acgcctatcc cgttgagcaa caccaagccc agcactcgca aacgccagcg
     2341 cgacgtcatc atcgcgtggg gctggccggc gcgtcgaaat aacccagcgc ttccgcggcc
     2401 aatcgccgcc cgagaatggc gtctgccgcc ttttcagcgg tcatgatcgt aggggcgttg
     2461 aggttgccgt tggtgatgtg agggaagatc gaggcgtcca cgacccgcag cccctggatt
     2521 ccgcgaacgc ggcactccga gtccaccacc gccattgggt cgttccccat gcggcaggtt
     2581 ccgcaggggt gataggcact ctccgtgctg cggcgcacga aggcatctat tgccccgtcc
     2641 gaatcggcgc cgctccccgg ggcgatctcg acaccccgat aagggtcgaa tgcccgctgg
     2701 gcgaagagct cccgggagag gcgaatcgcc cgccggaaca cctgccagtc ttcctcgcag
     2761 gacatgtagt tgaaactgat gacgggggcg acgtccgggt ctgccgagcg cagggtgagg
     2821 cggccgcggc tggggctcag catgggcccc acgtgcacct gaaaaccgtc cgcatcggcc
     2881 accgaccgcc cgtcgtagga cacggccgcc ggcagaaagt gaaactggac gtccggccag
     2941 gggacccgcg ctgagctgcg cacgaacccg ccggtctcga aatggttgga ggcccccgga
     3001 ccggaagcgg tgagcagcca ccgagcgccg atcaccccct tgcccaacgg gttgagccag
     3061 cggttgagcg acacgggctg agtgcacgcc tgctgaaaat agatctccag atggtccatg
     3121 aggttggcac ccaccccggg gagctcgtga cgcacgggaa tcccgtgagg ttcgagggct
     3181 tccccgggtc cgatgccgga gagcatgagc agctgaggag agccgatggc tcctgccgcc
     3241 agaaccacct cctcccgggc caccgcggtg tgggttgccc cccgatgccg gtaggtcaag
     3301 ccttcgcagc ggcccgattc cacgttcagc cgctgaaccg gggagcccga gcggaccgtc
     3361 aggttgctcc gcgagacaac aggggcgaga taggccaggg ccgcgctgca gcgaaccccg
     3421 tcgcgcaccg tcatctccag gtcaccaaag ccttcctgcc ggtaaccgtt gaggtcgtcg
     3481 ctgagcgcat gccccgcttg ctcagccgcc tggagaaagg tctcgtagag gggattggcg
     3541 cgccgcccgc ggctgacggt cagcggtccc gcccggcccc gataggggtc tccctcggca
     3601 gcgccgagca catgctcggc ccgcttgaag tagggcagga cgtccgcgta gccccatccc
     3661 gtagcaccca ggctctccca gcgctcgaag tcgcgcgggt ggccgcgcac gtaaaccatg
     3721 ccattgatgg acgacgaccc gcccagcacc cgcccccgcg ggcaggccag gcttctgccg
     3781 tcgagcgccg gctcggcctc cgcctgatag caccagtcga atcgcggccg gccaatgggc
     3841 agcgagaacg ccgaaggcat gcgaacgaac agatgccgat cgctgccacc ggcctctaaa
     3901 agcaacaccg atcgggcacc gtcctcggag agccggttgg cgagcacgca tcccgcggag
     3961 ccggcccctg caatgatgta gtcgaagact tccaacgatc ccgtaccctg cccagtcact
     4021 gctgcgcgtc tgcccgaggt gcaggctagc agacgccggg cggaaatggg gcacagcggc
     4081 gcggcggcgg ttagaattcc aggtcagcgc cccggggaag gaagatggtg agcggtagcc
     4141 tgctggccgg atggttcgcg ccgctggcgg tttatcttgc gatactgatc ctgcatctcg
     4201 ccctgcccgc gcggagggta gacgggtacg cgctggatcc cggcacgggc cagcctctga
     4261 cctaccgcct gaacggcctt gtcgtgttcg ccctcgtcac ttcggtctgg ctcgctgcag
     4321 gctggaaggg ctggatcagc tgggactggc tctacgtcaa ccgctggagc gcaatgggcg
     4381 gggcctgtct ggtcgggctg gcctacagcc tgatcaccgt gctcggcgcg cccccgaccg
     4441 gccggtcact tttagccgat ctctacctcg gcaggccgga aaatccgcag cacttcaaca
     4501 agcgtgtcga tgcgaagatg tttctctatc tcgcgggcgc cgttctgctc ggcctgaaca
     4561 ccctgtccgc cacggcgttt cacgcgggtg ccctgggtag cgccagcaac cccggcgtat
     4621 atctccatgc agctctgctc acttttttcg tgctggacta tctcgtcttc gagcgggttc
     4681 atctctacac ctacgacctg ttcgccgagc gcgtggggct caagctcggc tggggctgcc
     4741 tggccttcta ccccttcttc taccctgtgg gcctgtgggg caccgcccac ctgggcaagc
     4801 cgtcggtgat cgccgacctc ggttggatct ggctcgcgct gagcgcgctc gtttttctgt
     4861 ccggctgggt actggcgcgg ggcgcaaacc ttcagaagta cgttttcaaa agattcccgg
     4921 agcgggcgtt cctgggccga tttcctccgc gggtcattca cggcggcaac ctggccctgc
     4981 tgtgcggcgg gttctggggc gtcgcccgac acgtcaacta cctgggcgag atcctcatgg
     5041 ccacgggtct cgcgatgtcc ctcgggcatc tggacagccc ctggccctgg ctatatcccc
     5101 tgtactatct ggccctgctg gtgccgcggg agcgcgatga cgaccgtcgc tgtgcggaga
     5161 agtacgggac gctgtgggac gagtaccggc gccgggttcc ctaccggatc gtcccgcgga
     5221 tctattgacg cagcggagca cggacctgca catgaatctg gatctcatac ttgccctgaa
     5281 ggtgctcgcg ctggcgctga gcgccaatgg ggctccggtg atcggcaagc gcctgttcgg
     5341 cgcccgctgg gcgatccccc tggacggcgg tctcaccttt ctggacgggc ggcctctgct
     5401 ggggtcgagc aaaaccgtcc gggggctggt gctgagcctg gccaccacca ccctcgtggc
     5461 aacggcgcta ggctacggct ggcagctggg tgtgatctac tcggcgttga cgatgtccgg
     5521 ggacgcgctg tcgagcttcg tcaagcggcg ccggggcatt ccgcccagcg gtcggttccg
     5581 cggcctggat cagatccccg aaaccgctct gcccatgtgg gtctgcagct cggcgctggg
     5641 gctgacgggc tgggacatcg cggtgctcgt ggcgttgttc ttcgccggtg atttcgtgct
     5701 gtcgaaagta ctctaccgga tcggcatccg cgacgagccc tactgaagct gcgcgacgcc
     5761 agggcccgcc gaatgaaaga cgtgcaccgt aagctctggc ggacagttca ggcggacggc
     5821 gaccatggat gcccctgcgc cgggcgacgt gtacccccgc atgcctttgt aatcccagca
     5881 tccgcgtccg aggcgcctgg gaacgcgaga gtcgagcgtg accggataga cccccggcag
     5941 gcagatctga cccccatggg tatggccgca caacagcagc tgcacgccgg cgcgcgccgc
     6001 ctgccggtag acctcgggcg tatggctcag caggatcctg ggcaccgtgt cgggaacgcc
     6061 ggccagagcc ttctcgatgt cgtgcacgcc gaagaagtga gcatcgtcga tcccggcaag
     6121 gtagaggtga tcgtcaccgt gccggtgggc gtgactttcg ttcatgagca tgcgaatgcc
     6181 catgtcctcc agggcgggca gcatggtcac gctgtcgtgg ttaccgaaga cggcgtagac
     6241 cgatccgtcc aggttgctgc gcagccgggc catgccgcgc agggcatcgt cgatggcgcc
     6301 gtaggtgcgg aaacggtagt cgccggtcag cacgcagacg tcgtaggtca gctcccggac
     6361 cctttcgatg atggcgtgca gcagctcgtc gctcacgtcc acgtgcagat ccgagaggtg
     6421 caggacccgc agcccgtcta gaccggcggg aagagcgtcg agcaccacgt gattctccac
     6481 cacccgatga tcgagagcgt tcctgctggc gcgggcatac aggcccgaga ccttgagcga
     6541 gttgcgaatg atggaatgaa ccgagtacca gttctccggg tgaaaaaagc ggatcccatg
     6601 gctgaatagc cgagactcgt gagccgtctc cacgccgagg cgctcacggg cgtgggcggt
     6661 gcctaggcgt ttttccaggc gttgcagaat ctcgctgtcg cgcatctgaa gattatatat
     6721 gccacgcccc tgcccgctga cgtttcccgc tatccccgat cgccgccgaa ctcggggggg
     6781 cgtttttcca tgaacgccct ggccccctcc tgcatgttac gcgggatgag cactccctgg
     6841 tgcgccatct ccagcccaag ctgatcccca aaggtgtggt gaatggctgc atcaacgcat
     6901 tcgcgaatgc gggtgcacgc gctcggcgag gagcgcttca gaaccatcgc cacgcggccg
     6961 acttccgcgt cgagctcctc atccggcacc gagcgccaga tgaggcccca ggcttcggcc
     7021 tggttcgcgg tgatgcgatc gcccagcagc gtcatcccca gggcacgggc acgcccaacc
     7081 ctcatcggta tgctccaggt ggagccgaga tcgggcacga tgccgagatt ggggccgaag
     7141 gtcgccacga agattgccga ctccgcggcg atggcgacgt cgcaggccag cgccagcccg
     7201 agcccgccgc cggctgcagg accattgacc cgagcgatgg tcggcaccgg gcagctcatc
     7261 agcgcgctga ccgcgccgtt gaagagatcg ccgctgtccg gggcgtcctg caggtccggg
     7321 tctgccggcc cctccgccgc gcgggcgaga tccgcgccgg cgcaaaagcc gcgccccgcg
     7381 cccgtcaaga tgaccacgcg cacgctttcg tcttcgacaa cccgttccaa ggcccggcag
     7441 atgccgccca tgagctcgtc gttcatggcg ttgaggacct cgggccggtt cagaatgagc
     7501 gtggctgtgc cgtcgtcaat ctcgcagagg acgatttctg agtctgacat aggactcccc
     7561 catgaagagc ggatgaatca gctgcatccc atggaaccag aaacgtcccg gagcggacaa
     7621 gggctacgcg ccatcgcgcc gggcggggag gtcaataacc cgttggcggt ctcagaaccg
     7681 gaagttgaaa ccgacgaggg gcccgtggaa cccgtac
//
LOCUS       DBNHKV010000183         3162 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_309635, whole genome shotgun sequence.
ACCESSION   DBNHKV010000183 DBNHKV010000000
VERSION     DBNHKV010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_309635"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /gene="larB"
                     /locus_tag="AC16PP_05820"
     CDS             <1..466
                     /gene="larB"
                     /locus_tag="AC16PP_05820"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HIR6481181.1"
                     /translation="VPHARYHRDAELVMLKQSEPDPAPTKIAVVTAGTSDYRVAEEAA
                     LTAEFYDSPVIRISDVGVAGLHRLLSRIDAIRAARVVVVVAGMEGALPSVIGGLVDKP
                     VIAVPTSVGYGAAFDGVAALLGMLTSCASGVGVVNIDNGFGAGFLANMINRL"
     gene            477..1304
                     /gene="larE"
                     /locus_tag="AC16PP_05825"
     CDS             477..1304
                     /gene="larE"
                     /locus_tag="AC16PP_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HIR6481182.1"
                     /translation="MDAKLGAKYEKLKRRIAAFDSAIVAFSGGVDSSLVAYVTAEVLG
                     PRALAVTSGSKSLKRSDLHLAVELADKWGMQHRVIVTDELSKPDYRANPIDRCFHCKS
                     SLYAALTAIAREEGYAMIFSGTNCDDLGDHRPGLKAADDHGVTAPLVEAGFGKADIRA
                     LADELGLDNAQKPQSACLSSRFPYGTAIDENRLAQVEQAEDVLASFGFTQFRIRHHGD
                     VARLELLPDEIPAALDRAADIESAIRACGYRFVAVDLRGFRSGSLNEGMVKVVNLPA"
     gene            1378..2259
                     /locus_tag="AC16PP_05830"
     CDS             1378..2259
                     /locus_tag="AC16PP_05830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6481183.1"
                     /translation="MIEKIPFGRTGHESTRTLFGAAALGSMSPERAEGVLATLLEHGV
                     NHIDTAASYGDSELRLAPWLAANRASFFLATKTDQRTGSAARAQLERSLERLGVDQVD
                     LIQMHNLVDEQEWETAMAPGGALEALIDARAEGLVRFIGVTGHGTRVAAMHLKSLEQF
                     DFDSVLLPYNFTMMQQSDYAADVEALLKICKSRNVAVQTIKSVARRRWTDDDADKRFS
                     WYMPLRDRESLQRAVHFVLSRPGLFLNTSSDATILPLILEAASQPAEAPDPDAMRCDV
                     NALGMEPLFVRGVSDTI"
     gene            2272..>3162
                     /locus_tag="AC16PP_05835"
     CDS             2272..>3162
                     /locus_tag="AC16PP_05835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6481184.1"
                     /translation="MGQSIAIVGSGICGMCTAMTLAGQGHDITVFDKDVSPPAGNAEE
                     AFFSWERRGAAQFRHPHAFLGLMCNLIEERYPELLDAFYAAGARRVDLQEMLPAELRE
                     SYRPVRGDEKLWVLLCRRATIETIIRQYVGALPGIRIVNPCKVTDLITDSEGGRLQVK
                     GLMIQREHEGEVTPFQADVVIDASGRRTRFPRWFAQRGCTIPEEKHDAELVYYTRHYR
                     LKEGRSEPARSDRSGAGDLGYLKFGVFPGDNGHFAIILCVPTPETAIRKAVRSGDLFD
                     RICLSIPGLEPWLADGRAEAT"
BASE COUNT          584 a         1003 c         1019 g          556 t
ORIGIN      
        1 agtgccgcac gcacgctacc accgtgatgc ggagctggtg atgctgaagc agagcgagcc
       61 cgaccccgcg cccacgaaga tcgccgtcgt caccgccggc acgtccgact accgggtggc
      121 cgaggaagcc gcgctgaccg ccgagttcta cgacagcccg gttatccgca tcagcgacgt
      181 cggcgtggcc ggcctgcaca ggttgctgag ccggatcgac gccatccgcg ccgctcgggt
      241 ggtcgttgtg gttgcgggga tggagggcgc tctgcccagc gtgatcggag ggctggtgga
      301 taaacccgtg atcgccgtgc ccacgagcgt cggttacggc gccgcgttcg acggggttgc
      361 cgccctgctc ggaatgctga cgagctgtgc atccggcgtc ggcgtcgtta acatcgacaa
      421 cggctttggc gcgggtttcc tcgccaacat gatcaaccgc ctctgagaga ctgcacgtgg
      481 acgccaaact tggtgcgaaa tacgagaagc tgaaacgtcg aatcgcggcg tttgatagcg
      541 cgatcgtcgc gttctcgggc ggcgtcgaca gctcgctcgt cgcgtacgtt accgctgaag
      601 ttctggggcc cagggcgctg gcggtaactt ccggctcaaa gagcctgaag cggagcgacc
      661 ttcatcttgc cgtcgagctg gccgacaaat ggggtatgca gcatcgcgtc atcgtcaccg
      721 atgagctcag caagccggat tatcgggcca atcccatcga ccgctgcttc cactgcaaat
      781 cgtctctgta cgccgcgctg acggccatcg cccgggaaga gggttacgcc atgatcttca
      841 gcgggacgaa ttgcgacgat ctgggcgatc accggccggg cctcaaagcg gcagacgatc
      901 acggcgtgac cgccccgctg gtcgaggccg gtttcggaaa ggccgatatc cgggctttag
      961 ccgacgagct cggattggac aatgctcaga aaccccaatc cgcctgcctt tcgagtcgtt
     1021 ttccctacgg aacggccatc gacgagaacc gattggcaca ggtcgaacag gccgaggatg
     1081 tgctggcgtc tttcggtttt acgcagttcc gtatccggca tcacggggac gtggcgcggc
     1141 tcgaactgct gcccgacgag atacccgccg cgctcgatcg tgcggcggac atcgagtcgg
     1201 ccattcgcgc ctgcggctac cggttcgttg ccgtggacct gcgaggtttc cggtccggat
     1261 ctctgaacga aggcatggtc aaggtggtca acctgcccgc ctgacccgga gggctcgttg
     1321 caccgtcctg gaccgttggg ccatgattgc agtcactcga gcaagcagcg gatcgacatg
     1381 atcgagaaaa ttccttttgg ccgcacgggc cacgagagca ccaggaccct attcggcgcg
     1441 gccgcgctgg gaagcatgag cccggagcgc gccgagggcg tcctggccac cctgctggag
     1501 catggggtca atcacatcga cacggcggca agctacggcg attccgagct gcggctggcg
     1561 ccctggctgg cagcaaatcg ggcgagcttc tttctcgcca ccaaaaccga ccagcggacg
     1621 ggctccgcag cccgtgcaca gctcgaaagg tcgctggagc gcttaggcgt ggatcaggtc
     1681 gatctcattc agatgcacaa tctggtcgac gagcaggagt gggaaaccgc aatggccccc
     1741 gggggcgccc tggaggcact gatagacgcc agagccgaag gcctggtccg cttcatcggc
     1801 gtcaccggtc acggtacccg cgtcgccgcg atgcatctga aaagcctcga gcagttcgac
     1861 ttcgactccg tgctcctgcc ctacaacttc acgatgatgc agcaatctga ctacgcggca
     1921 gacgtggaag cgctgctgaa gatctgcaaa tcccgaaacg tcgcggtgca gacgatcaag
     1981 tcggtggcgc gccggcggtg gacggacgac gacgcggaca agcgtttcag ctggtacatg
     2041 cccctgcgcg accgggaaag ccttcagcga gccgtgcact tcgtcctctc ccgccccggc
     2101 ctcttcctca acacctccag cgacgcgacc atacttccgc tgatcctgga ggccgcgagc
     2161 cagcctgccg aggcgccgga tccggacgcc atgcgttgcg acgtgaacgc cctagggatg
     2221 gaaccgctgt tcgtccgcgg cgtctctgac acgatctgac gcaacaaccc tgtgggccag
     2281 agcatcgcca tcgtcggttc gggcatctgc gggatgtgca cggccatgac gctcgcgggg
     2341 caagggcacg acatcaccgt gttcgacaag gacgtctcgc cacctgcagg caacgcagag
     2401 gaggcttttt tcagctggga gcgtcggggc gccgcgcagt tccgacaccc gcatgcgttt
     2461 ctgggcctga tgtgcaacct gatcgaggag cgctaccctg agcttctcga tgccttttac
     2521 gctgctggag cgagacgggt cgatttacag gagatgctcc cggcggagct gcgagagagt
     2581 taccgacccg tgaggggtga cgagaagctc tgggtcctgc tctgtcgccg agccaccata
     2641 gagaccatca ttcgccagta cgtgggcgct cttcccggga tcaggatcgt gaatccctgt
     2701 aaggtcacgg acctcattac agactcggaa gggggccgtc tgcaggttaa ggggctcatg
     2761 attcagcgtg agcacgaagg cgaggtgaca ccgtttcaag cagacgtcgt catcgacgcg
     2821 agcggacggc gaacccgctt tccgcggtgg ttcgcacaac ggggttgcac catccccgag
     2881 gaaaaacatg atgcggagct cgtctactac acgcggcatt accgcctgaa ggagggccga
     2941 tcggagcctg cccgaagcga ccgttccggg gcgggcgatc tggggtacct caagttcggc
     3001 gtgttccctg gcgacaacgg ccacttcgct atcattctgt gcgtgcccac gcccgagacc
     3061 gccatcagaa aggccgtccg cagcggcgac ctgttcgaca ggatctgctt gagcattcca
     3121 ggcctcgaac cctggctggc cgatggccgc gcagaggcca cc
//
LOCUS       DBNHKV010000184         4891 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1088342, whole genome shotgun sequence.
ACCESSION   DBNHKV010000184 DBNHKV010000000
VERSION     DBNHKV010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1088342"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="AC16PP_05840"
     CDS             <1..257
                     /locus_tag="AC16PP_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6481185.1"
                     /translation="EEVARSLALSKAREMAAVAGLEAADVHVMVGSPSGQIVAAAEAH
                     GADLIVVGSHGKHGLGLLLGSTANGVLHHAETDVLTVRIT"
     gene            270..737
                     /locus_tag="AC16PP_05845"
     CDS             270..737
                     /locus_tag="AC16PP_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6481186.1"
                     /translation="MAISYRNVLIAIDASDEADEVLEAATTLDPRGAARFQVCTVIPP
                     VMGGVSGMDGASFAATWPLREMEETITKDISSHVRERVARFGITPDRVAVLYGRPAVE
                     IRAYAAKIGADLIVIGSHGRHGVPGVLLGSTANAVLHGAECDVLTVRVRPAEG"
     gene            847..2211
                     /locus_tag="AC16PP_05850"
     CDS             847..2211
                     /locus_tag="AC16PP_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR6481187.1"
                     /translation="MKAWLPILIACAGLALGFGEARPATGAQPAADGVVWLVDLDGPL
                     GPATADLIIRSLDDAQEAGARALVIRMDTPGGLDKAMRGLIKEMLSADLPVVTFVGPD
                     GARAASAGTYIAYASHIAAMAPATNIGSSTPVSIAPTPQMPDPRDAGETEGDAGRAPS
                     GDAMTRKVVNDAVAYLQGLAELRGRNIDWAEETVRLGANLRASEALEANVVDLITRDV
                     SSLLADIDGWVVELPNRTVTLDVANARVHTVETDWQHELLEVITDPTIAYGLLIFGVY
                     GLILEFYNPGMVFPSVIGIICLLLGAYGLQMLPVNYAGLALIVVGIGMMIAEVFTPTM
                     GVLGLAGVVAFVAGSMILMDTEAPGYGIPITVVAAFAAASAGLTIFAIGAAMKARRAK
                     IVTGVEAMIGASGEALEDFQGTGHVRAFGEIWEARCSEPVAAHAKVRVTEVDGLILVV
                     QPED"
     gene            2215..2994
                     /locus_tag="AC16PP_05855"
     CDS             2215..2994
                     /locus_tag="AC16PP_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015275390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIR6481188.1"
                     /translation="MEIFSTVIAVIIVGLLISAFRVLREYERGIVFMLGRFYKVKGPG
                     LIIIVPVIQQMVRVDLRTIVLDIPSQDLITRDNVSVKVNAVLYFRVVDPERSIINVED
                     YMEATGQLAQTTLRSVLGQHELDELLAERERLNSDVQAILDKQTDQWGIKVANVEIKH
                     VDIDESMIRAIAKQAEAERERRAKVIHAEGEAQAASKLVEAAQLLASQGEAMQLRYLQ
                     TLTEIAGEKSSTIVFPLPMNVMQSIERILEHSGVPKSGQSD"
     gene            complement(3011..3913)
                     /locus_tag="AC16PP_05860"
     CDS             complement(3011..3913)
                     /locus_tag="AC16PP_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6481189.1"
                     /translation="MKQINNILVAGDSLNGMEAALAKAAKIEHYTGANVWVAVVIYDA
                     IAEEDPDRLPPEQQSQLVEALKAAERNGLRQLTEPYRSRIATLDDRVVWNKNAAEGLL
                     RVQTEVHADLIIKPISEHGFITDHLHTPLDWALMRGSQGAVLISKKSDWANPQRVLAA
                     IDVADTRHADLSREILLTAATMARTLDAELHVVTAFPSLGQTVNDLQVAMDYEGIKRD
                     MRENRTAAIARWIEELDIGISDSHVLEGKPELVIPQLASDLSATLTVLGTAARQGIRK
                     LLIGNTAENIIARLDCDILTVREP"
     gene            4054..>4891
                     /locus_tag="AC16PP_05865"
     CDS             4054..>4891
                     /locus_tag="AC16PP_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6481190.1"
                     /translation="MDSINNILVALSDTAEARFVLEKVLILAKAGQAKVHVVSVVYEG
                     IAELTIDEIEDSARLKTLILESAESALEELVDPLRAQLPAIECATLWNRNAWEGVLHA
                     AEAVEADLIVKPATLHEKFGDVVRTPDDWNLLRHASVPVMLVKPQAWVDEPVLLCAVD
                     PFDDAHANLNAAMLREGRALADMLGGELHVVCAYPLFEPWVGELGAVRSYAEIKENVE
                     REIIRRVDAIAARAGVKFAMLHAEEGYPTHTIGAVAELTGAELVILGTHAREGVRGLL
                     LGN"
BASE COUNT          861 a         1491 c         1623 g          916 t
ORIGIN      
        1 tcgaagaagt agcgcgcagc ctggcgcttt ccaaagcccg cgagatggcc gctgtcgccg
       61 gcttggaggc tgcggacgtg cacgtgatgg tgggctcccc gtcggggcag atcgtcgcgg
      121 ccgctgaggc tcacggagcc gatctcatcg tcgttggcag ccacggaaag cacggtcttg
      181 gtctcctgct cggatcgacg gccaacggtg tgctgcacca cgcggaaacg gacgtcctga
      241 ccgtccgtat cacctgagcg gcggcggcta tggcgatcag ctacagaaac gtcctgattg
      301 cgatcgacgc gtccgacgag gctgacgagg tgctggaggc cgcgaccacc ctcgatcctc
      361 gcggggcagc ccgctttcag gtctgcacgg tcattcctcc ggtcatgggc ggtgtgagcg
      421 ggatggatgg cgcatcgttc gctgccacct ggccgctgcg cgagatggag gaaaccatca
      481 caaaggatat ctccagccac gtgcgtgagc gtgtggcgcg gttcggcatc acgccggacc
      541 gcgttgccgt actctacggc aggccggcgg tcgaaattcg cgcttacgcc gcgaagatcg
      601 gagccgacct gatcgtcatc gggtctcacg gtcgccacgg ggtgcccggc gtgctgcttg
      661 gttccacggc caacgcagtg ctgcacggcg ccgaatgcga tgtgctgacc gtgcgggtgc
      721 ggccggcaga aggttaggtc cgctcattgc ccgcggctag gccgtgggcg agttcgatca
      781 gccgatcccg gcaataaggc gtacaatgct gcaacgttcg ggaacttgga cattgctgct
      841 cccaccatga aggcctggct accgattctc atcgcttgtg ccggacttgc cctgggcttc
      901 ggggaagccc gtccagccac cggggcgcag cccgcggcgg acggtgtggt gtggctggtg
      961 gatctcgacg gtcccctcgg tcctgccacg gcggatctca tcatccgctc gctcgatgat
     1021 gcccaggagg ccggggcccg cgcgctggtg attcgaatgg acacaccggg agggctggac
     1081 aaggccatgc gcgggctgat caaggagatg ctttcggcag acctgcccgt ggtcaccttc
     1141 gtcgggccgg acggcgcccg tgcggcaagc gccggaacct acattgctta cgccagccac
     1201 atcgcagcca tggcaccggc taccaacatc ggttcctcta cgcccgtcag catcgcgccg
     1261 acgcctcaga tgcccgaccc gcgtgacgcc ggcgaaacgg aaggcgacgc cgggcgggca
     1321 ccgtcggggg acgccatgac acgcaaggtc gtgaacgacg ccgtagccta tctgcagggg
     1381 ctggcggagc tcaggggccg gaatatcgat tgggccgagg agacggtgcg gcttggcgcc
     1441 aacctgcgcg cctcggaagc tctggaagcc aacgtggtgg atctcatcac gcgggacgtc
     1501 tcctcgcttc ttgccgacat cgacgggtgg gtggtggagc ttcccaatcg gaccgtcacg
     1561 ctcgatgtgg caaacgcgcg cgtgcacacc gtggagaccg actggcagca cgagcttctc
     1621 gaagtgatca cggatccgac gattgcctac ggcctgctga tcttcggcgt ctacggcctg
     1681 atactggaat tctacaatcc cgggatggtc tttccctcgg tgatcggaat catctgcctg
     1741 cttctgggcg catacggtct gcagatgctt ccggtcaact acgcgggcct ggcgctgatc
     1801 gtggtcggca tcggcatgat gatcgcggag gtcttcacgc caaccatggg tgtgctgggc
     1861 ctcgccgggg tcgtcgcgtt cgtcgcgggc tcgatgatcc tgatggacac cgaggcgccc
     1921 ggctacggca tcccgattac cgtggtggcc gcattcgcgg cagccagtgc gggcctgacg
     1981 atttttgcca tcggggctgc gatgaaggcc cgtcgggcga agatcgtaac cggcgtggaa
     2041 gcaatgatcg gcgcctcggg agaagcgctg gaggactttc aggggacggg ccacgttcga
     2101 gcattcggcg agatctggga ggcacgctgc agcgagcccg tggcggcaca cgcgaaagtg
     2161 cgcgtaaccg aagtagacgg gctgattctg gtcgttcaac cggaggattg attaatggaa
     2221 atattttcga cagtcatcgc ggtcatcatc gttggtcttc tgatcagcgc tttccgggtc
     2281 ctgcgggagt acgagcgggg catcgtcttc atgctcggac gcttctacaa ggtcaagggg
     2341 cccggtctga tcatcatcgt tcctgtcatt cagcagatgg ttcgggtgga cttgagaacc
     2401 attgtgctcg acatcccctc gcaggatctc atcacccgcg acaacgtgtc cgtcaaggtc
     2461 aacgccgttc tctatttccg ggtcgtcgat ccggagcggt cgatcatcaa cgtcgaagac
     2521 tacatggaag cgaccgggca actcgcccag accacgctcc ggtcagtgct cggccagcat
     2581 gagctcgacg agctgctggc ggagcgggag cggctcaact cggacgtcca ggcgatcctg
     2641 gacaagcaga ccgatcagtg gggcatcaag gtcgccaacg tggagatcaa gcacgtcgac
     2701 atcgacgaga gcatgatccg ggcaattgcc aagcaggcgg aagccgagag agaacgccga
     2761 gccaaggtca ttcacgcgga gggcgaagcg caggccgcct cgaagctggt ggaggctgct
     2821 cagcttctcg cctcacaggg agaagccatg cagctgcgct acctgcagac gctgacggag
     2881 atcgccggag agaaaagctc caccatcgtc ttcccgttgc cgatgaacgt catgcagtcc
     2941 atcgagcgaa tactggagca cagcggcgtg ccgaagtcgg gtcaatcgga ctgaacgctc
     3001 gacgaagttc tcacggctcg cgtacggtga gaatgtcgca atcgagccgg gcgatgatgt
     3061 tttccgcggt gttcccgatt agcagtttcc tgatgccctg tcgcgcggcg gtgcccagga
     3121 cggtgagcgt cgcggagaga tcgcttgcca actggggtat cacgagttcg ggcttgccct
     3181 ccagcacgtg tgagtcgctg atcccgatgt cgagctcctc gatccatcgg gcgatcgcgg
     3241 cggtccggtt ctcacgcatg tcgcgcttga tcccttcgta gtccatggct acctgaaggt
     3301 cgttgaccgt ctggcccagg ctcgggaagg cggtcacaac gtgcagctcc gcgtccagag
     3361 tccgggccat ggtcgctgcg gtcagaagaa tttcgcggct gaggtcagca tggcgggtgt
     3421 cggcaacgtc gatggcggcc aggacccgct gggggttggc ccagtccgac tttttcgata
     3481 tcagcaccgc gccctgggat ccccgcatca acgcccagtc cagcggtgtg tggagatggt
     3541 cggtgatgaa gccatgctcg gaaatcggct tgatgatgag atccgcatgg acctcggtct
     3601 gaacccggag cagcccttcg gccgcgttct tgttccagac gacccggtca tcgagcgtgg
     3661 cgatccttga ccggtacggt tccgtcagct gacgaaggcc gtttctttcc gctgccttga
     3721 gcgcctcgac gagttgtgat tgctgctcgg gaggcagcct gtccggatct tcctcggcga
     3781 tggcgtcgta aatcaccacg gcgacccaca cgttcgcgcc ggtgtagtgc tcgatcttcg
     3841 ctgccttggc cagggcagct tccatgccgt tcagcgagtc gccggctacg agtatgttgt
     3901 tgatctgttt catgtcgatg aacccatttc acgccgcctc cgggcgcggg tcagtacgta
     3961 cataccgaag cgccagtgga aactacggat acgccgcggc gagcccgcca cctatgatca
     4021 attccacctg aatctgccgg tggggttcga gcaatggatt ctatcaacaa catcctcgtg
     4081 gcgctcagcg acacggccga ggcgcggttc gtcctggaga aggtcctgat actcgcgaag
     4141 gccggtcagg cgaaagtgca tgtggtgagc gtcgtttacg aaggcatagc cgaactgacg
     4201 atcgacgaga tcgaggactc ggcacgtctg aaaacgctca ttctcgaatc cgccgagtcg
     4261 gcgcttgagg agctcgtgga cccgctgcgt gcccagctgc cggcgatcga atgcgcgact
     4321 ctttggaatc gcaacgcctg ggagggcgtc ctgcatgccg ctgaggccgt ggaggcagac
     4381 ctcatcgtca agccggccac ccttcacgag aaattcggag acgtggtgcg tacccccgac
     4441 gactggaatc tgctgcgcca cgccagcgtg cccgtgatgc tggtcaagcc ccaggcctgg
     4501 gttgacgagc ccgtcctgct ctgcgcggtg gatcctttcg acgatgctca cgccaacctg
     4561 aacgcggcga tgctgcgcga gggccgggcg ctggcggaca tgctgggcgg cgagcttcac
     4621 gtggtctgcg cttatccgct gttcgagccc tgggtgggtg agctcggggc ggtcagaagc
     4681 tacgcggaaa tcaaggagaa tgtcgagcgc gagatcatcc gccgcgtcga cgccatcgct
     4741 gcccgagcgg gcgtgaagtt tgccatgctg catgccgagg aggggtatcc cacccacacc
     4801 atcggggcgg ttgccgagct aaccggggct gaactcgtca tcctaggtac ccacgcccgg
     4861 gaaggcgtgc gggggctgct tctgggcaac a
//
LOCUS       DBNHKV010000185         6686 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1300838, whole genome shotgun sequence.
ACCESSION   DBNHKV010000185 DBNHKV010000000
VERSION     DBNHKV010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6686)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1300838"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="AC16PP_05870"
     CDS             complement(<1..339)
                     /locus_tag="AC16PP_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016391145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR6481191.1"
                     /translation="MVATARTAALAGGSDRRTFAIISHPDAGKTTITEKLLLFGNAIQ
                     LAGTVKARKSDRHATSDWMAMEQQRGISVTTSVMQFPYRDRVVNLLDTPGHADFSEDT
                     YRTLTAVDSAL"
     gene            complement(404..898)
                     /gene="rimI"
                     /locus_tag="AC16PP_05875"
     CDS             complement(404..898)
                     /gene="rimI"
                     /locus_tag="AC16PP_05875"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR6481192.1"
                     /translation="MDQDPESRALINLRPMETADLDAVLEAEFLSYAFPWTRGVFLDC
                     LKARHECWVGCLGDAPVAHAVVSVGAGESHLLNLCVRRDLQGRGYGRALAVHVVGRAR
                     ARGASAMFLEVRPSNLVAAELYESLGFREIGIRKNYYPAQVGHEDARVLALDLDAHFR
                     SDPL"
     gene            complement(868..1524)
                     /locus_tag="AC16PP_05880"
     CDS             complement(868..1524)
                     /locus_tag="AC16PP_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481193.1"
                     /translation="MTEAVRLDARRRGMLADMGIDVWVIRGNAAGVVEVPQSQTIVVP
                     EPVPPAETNAPEKGPDVARLNDSAPSAAQQAKMPVPRRRPLEHPEADVATEIAVTCLT
                     RPGALLLAYGREWTQAGRLGRDLLAAATGSWGSDPKRISFGWREPGDPSDGWRAFKAF
                     ADKQLADAEASVIFVNEALVDHLPERASSGTLVVLPNLAEFDTDAKRGLWTRIRNLGR
                     "
     gene            1634..2719
                     /locus_tag="AC16PP_05885"
     CDS             1634..2719
                     /locus_tag="AC16PP_05885"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR6481194.1"
                     /translation="MKYLTDDVRIIGMEEVTAPETLLSELPITDSVSRLVFSNRKQIS
                     EILHGSDPRLLVVVGPCSIHDPEAALDYAKRLAPVAKDLSDRLHVIMRVYFEKPRTTV
                     GWKGLINDPHLDNSFDINNGLRIARGVLRDIAECGLGAGTEYLDPITPQYVGDLVAWG
                     AIGARTTESQIHRQLASGLSCPVGFKNSTDGSIQVAVDAIKSAANSHIFLSVTKSGHS
                     AIFSTAGNDDCHLILRGGGGKPNYDNPSVHYASRLLEQAGLTSRLMIDMSHANSGKDH
                     NQQIRVCQDICKQVREGESRIFGVMIESNLVPGNQKIGPRESMTYGQSVTDACIGWED
                     TVTCLTALADAGGARMEGSAAAAAGSG"
     gene            complement(2716..3087)
                     /locus_tag="AC16PP_05890"
     CDS             complement(2716..3087)
                     /locus_tag="AC16PP_05890"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR6481195.1"
                     /translation="MAHGGDAKRLAEMRCEPCRGDSPAVAPDAREAMLAELTGWRVDV
                     VDEVPRLSRTYQTADFAAALGLANTIGEMADSEDHHPQLVVEWGRLQVSWWTHAIGGL
                     HMNDFVLAARCDELAAKAASD"
     gene            complement(3053..3652)
                     /locus_tag="AC16PP_05895"
     CDS             complement(3053..3652)
                     /locus_tag="AC16PP_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013918.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016679 - oxidoreductase activity, acting
                     on diphenols and related substances as donors [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HIR6481196.1"
                     /translation="MRRRLLFTALCLPLAWLGYAIAGELNQPGSVLGADPGEAVVLFL
                     GEWGLRLLLLTLAVTPLRRLTGWAELLGYRRMIGLFSFTYLALHLLAYVALLAEFDWR
                     LVLEDFSDRAYITAGLIGFGLLLPLAVTSTRGWQRRLGRRWRSLHRLVYPAVFLGLLH
                     LVWLTKDGYGEVAAYGVLLSLLMVERIRHGAWWGRKAAG"
     gene            complement(3657..4583)
                     /gene="msrP"
                     /locus_tag="AC16PP_05900"
     CDS             complement(3657..4583)
                     /gene="msrP"
                     /locus_tag="AC16PP_05900"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HIR6481197.1"
                     /translation="MLIRRPDDVRPSEITPEQVYLNRRKFAKALGAGAAMLAAPWSAN
                     AGDSSGNDLEPTPEDIVTSYNNFYEFGTGKEDPKKHAHALTTSPWSVEVSGAVGKPGT
                     YDLDDLLKGLAPEERIYRFRCVEAWSMVVPWLGIPLKSILDRLEPLGSAKYVEFKTLY
                     RPSEMRGQRSPFSSIDWPYVEGLRLDEANHPLTIMAVGLYGKPLPNQNGAPWRLVVPW
                     KYGFKSIKSIVSIRFTEREPENTWKLLQPQEYGFYANVNPAVDHPRWSQASERRLPSS
                     LFSQNRMPTLPFNGYADEVAGLYAGMDLTRYY"
     gene            complement(4653..5456)
                     /gene="pssA"
                     /locus_tag="AC16PP_05905"
     CDS             complement(4653..5456)
                     /gene="pssA"
                     /locus_tag="AC16PP_05905"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HIR6481198.1"
                     /translation="MSDSEGNQERPPLHCVEEEAGSGSGAASANRMRGIYLLPNLITT
                     GALFAGFYAIVAGMNEHLTAAALAIFAAMALDTADGRVARRTRTESQFGAEYDSLSDM
                     VAFGVAPALVAFSWGLGDALGRIGWVVTFVYMACAALRLARFNVKSDVSSFTGLASPS
                     AAAIIACSVWAWSDNIGGDPGRAAAAGMGAITVVLGLLMVSNFTYYSPKALNMKGRVP
                     FVTMVLVALIFAVTLADPPLVLLFLFSLYALSGPGQWAWGRLTRRGSKG"
     gene            complement(5541..6560)
                     /gene="ilvC"
                     /locus_tag="AC16PP_05910"
     CDS             complement(5541..6560)
                     /gene="ilvC"
                     /locus_tag="AC16PP_05910"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR6481199.1"
                     /translation="MQVYYDKDADLSIVQKMNVLVVGYGSQGHAHANNLRDSGVANVT
                     IGLRSDSGSARKATNAGFQVADVADAAKDADLIMILTPDEHQPQIYRDQIAPNVKQGA
                     TLAFAHGFNIHFELIEPRADLDVIMIAPKGPGHTVRSTYVEGGGVPSLIAIFQDASGQ
                     AKNTALSYASANGGGRAGIIETTFQEETETDLFGEQAVLCGGAAALVQAGFETLVEAG
                     YAPEMAYFECLHELKLIVDFFYEGGITNMWYTVSNTAEYGGLTRGPRLVNEETKQEMR
                     RILEEIQTGRFAQEFVAENQAGAPRIKAMRRISQAHEIEQVGERLRAMMPWIQENRLV
                     DKDKN"
BASE COUNT         1259 a         2248 c         2031 g         1148 t
ORIGIN      
        1 cagcgccgaa tccaccgcgg tcagggtacg gtaagtgtcc tcggaaaaat ccgcgtgccc
       61 cggggtatcg agcaggttca ccacccggtc ccgataggga aactgcatca ccgaggtggt
      121 gacggaaatc ccccgctgct gttccatggc catccagtcg gaggtcgcat ggcggtccga
      181 cttgcgcgct ttcaccgtgc cggcgagctg gatggcgttg ccgaacagga gcagcttctc
      241 ggtgatcgtg gtcttgccgg catccggatg agagatgatc gcaaaggtgc gtcgatcgct
      301 cccccccgcg agcgcggcgg tccgggccgt tgccaccatg ataaagactc ctcagcgacc
      361 gacttttccg aagccggacg gccccgccgg aaaacggcgc ggattatagc gggtcgctgc
      421 ggaaatgagc atcaagatcc aacgcgagta cccgggcgtc ctcatgaccc acctgggccg
      481 gatagtaatt cttgcgaatt ccgatttccc ggaacccgag cgactcgtac agttcggcgg
      541 ccaccagatt agagggccgg acctcgagga acatggccga cgcgccccgc gcccgggccc
      601 gccccacgac gtgcaccgct agagcacggc cgtaaccccg tccctgaagg tccctgcgaa
      661 cacacagatt gagcagatgc gactcgccgg cccccacgga caccacggcg tgcgccaccg
      721 gagcgtcgcc gaggcagccg acccagcatt cgtgccgagc cttcagacaa tccagaaaga
      781 ccccgcgcgt ccacggaaac gcgtagctta aaaactcggc ttccagaacg gcatcgagat
      841 ccgccgtctc catgggccga aggttgatca gcgcccgaga ttccggatcc tggtccatag
      901 gcctcgtttg gcgtcagtgt cgaactcggc taagttcggc agcacgacca gcgtcccgga
      961 gctcgcccgc tccggaaggt gatcgaccag cgcttcgttt acgaaaatca ccgaagcctc
     1021 ggcgtccgcc agctgcttgt ccgcaaatgc cttgaaggcc cgccagccgt ccgaaggatc
     1081 gcccggctcc cgccagccga atgagattcg ctttggatcc gagccccagc tgccggtcgc
     1141 ggcagcgagg aggtcgcggc cgagcctgcc ggcttgggtc cattcccggc cgtaagccag
     1201 cagcaaagcg ccggggcgcg tcagacaggt gaccgcgatt tccgtcgcga catccgcttc
     1261 cggatgttcc aagggtctcc ggcgcgggac cggcatcttc gcctgctggg ccgccgaagg
     1321 cgccgagtcg ttcagcctgg caacgtcggg gcccttctcc ggcgcattcg tctcggccgg
     1381 cggcacgggc tcgggcacga cgatggtctg cgactgcggg acctcgacga ccccggccgc
     1441 gttacccctg ataacccaga cgtctatgcc catgtccgcg agcatccccc gacgccgcgc
     1501 atccagacga acggcttcag tcatgcccgc acccgttcag aactttggta tacatgccgc
     1561 gattgccggt aaccctgcct ctgcggtcat tgtacactct agcttccgag ctcacgcacc
     1621 gtgatggttc ccaatgaagt atctgacaga cgacgtacgc atcatcggca tggaagaggt
     1681 caccgcgccg gaaaccctcc tttccgagct gccgatcacc gattccgttt cgcgactggt
     1741 cttctccaac cgcaagcaga tcagcgagat tctccacggg agcgaccccc gattgctggt
     1801 ggtcgtcggg ccctgctcga ttcatgaccc ggaagctgcc ctggactatg caaagcggct
     1861 ggccccggtg gcgaaagacc tctccgaccg tcttcacgtc atcatgcggg tctacttcga
     1921 aaaaccgcgc acgaccgtgg ggtggaaggg cctgatcaac gacccccatc tcgacaactc
     1981 cttcgacatc aacaatggtc tgaggatcgc caggggcgtg ctcagagaca tagccgaatg
     2041 cggactgggc gcgggcacgg agtacctgga tccgatcacg ccgcagtatg tcggcgacct
     2101 ggtagcctgg ggcgctatcg gagcccgcac caccgagagc cagattcacc gccagctcgc
     2161 ttccgggctg tcctgccccg tgggtttcaa gaacagcacc gacggctcca tccaggtcgc
     2221 cgtggacgcc atcaagtcgg ccgccaattc ccacatattt ctctccgtca ccaagtccgg
     2281 gcactcggcc atcttttcca cggccggcaa cgacgattgc catctcattc ttcgaggcgg
     2341 gggcggcaag ccgaactacg acaacccttc ggtccactat gccagccgtc tgctcgagca
     2401 ggcggggcta acgagccgcc tgatgatcga catgagccac gccaacagcg gcaaggatca
     2461 caaccagcag atccgggtct gccaggatat ctgcaagcag gtccgcgaag gcgagtcacg
     2521 gattttcggc gtgatgatcg agagcaacct ggtcccgggc aaccagaaga tcggcccacg
     2581 tgaatccatg acgtacggcc agagcgtcac tgatgcgtgt atcgggtggg aggacacagt
     2641 cacctgcctg accgcgctgg ccgacgcggg cggggcgcgg atggaaggct cggccgctgc
     2701 agcggcagga tcgggctagt ccgaagccgc cttggcagcc agctcgtcgc atcgggccgc
     2761 gagaacgaag tcgttcatgt gcaggccgcc gatggcgtgc gtccaccagc tgacctgcag
     2821 ccggccccac tccaccacca gctgcggatg gtgatcctca ctgtcggcca tctcgccgat
     2881 ggtgttcgca agccccagcg ccgcggcgaa gtcggcggtt tgataggtcc ggctgagcct
     2941 gggtacctcg tccaccacgt cgacccgcca gccggtgagc tcggccagca tagcctcccg
     3001 cgcatccggc gccactgccg gcgaatcgcc ccgacaaggc tcgcaccgca tctcagccag
     3061 ccgctttgcg tccccaccat gcgccatgtc tgatcctctc taccatcaat agactcaata
     3121 gtacgccgta tgccgcaact tcgccgtacc cgtccttggt gagccagacc agatggagca
     3181 gcccgaggaa cacggccggg tagaccagcc ggtgcaggct gcgccagcgg cgacccaggc
     3241 ggcgctgcca gccgcgggtc gacgtgacag ccagtggcaa cagcagcccg aacccgatga
     3301 gcccggccgt gatgtaggct cgatcagaga agtcctcgag caccagacgc cagtcgaact
     3361 cggcaagcag cgcgacgtag gcaagcaggt gcagagccag gtaggtgaag ctgaacagac
     3421 cgatcatccg gcgataaccg agcagctccg cccacccggt caggcgccgt aagggcgtga
     3481 ccgccagtgt gagaagaagc aggcgcaagc cccactcgcc gagaaagagc accacagcct
     3541 ctcccggatc tgcccccagc acgctcccgg gctgattcag ctcgccggcg atcgcgtaac
     3601 ccagccacgc gagcggcagg cacagggccg taaacagcag gcgtctgcgc acgggctcag
     3661 taatagcggg tgagatccat ccccgcatac agacccgcca cctcatcggc atagccgttg
     3721 aagggcaacg tcggcatccg gttctgcgag aacagggagc tcggcagccg tcgttccgat
     3781 gcctggctcc agcgcgggtg gtcaaccgcc ggattgacgt tggcgtagaa gccgtattcc
     3841 tgcggctgaa gcaatttcca ggtgttctca ggctcacgct cggtgaagcg aatggagacg
     3901 atggacttta tgctcttgaa gccgtacttc cacggcacga cgaggcgcca cggcgccccg
     3961 ttctggttcg gcagcggctt gccgtagagg ccaacggcca tgatcgtgag ggggtgattg
     4021 gcctcgtcca gccgcaagcc ctccacgtac ggccagtcga tgctgctgaa cggcgagcgc
     4081 tggccacgca tctcgctggg gcggtacagc gtcttgaact cgacgtactt cgcgctgccg
     4141 aggggctcaa ggcggtcgag aatggacttg agaggaatgc caagccatgg aacgaccatc
     4201 gaccaggctt caacgcagcg aaaccgatag atgcgttcct ccggcgccag ccctttgaga
     4261 agatcatcca gatcgtaggt gcccggtttg cccaccgccc ccgaaacttc cactgaccag
     4321 ggcgatgtgg tcagcgcgtg ggcgtgcttc ttggggtctt cctttccggt gccaaactca
     4381 tagaagttgt tgtagctggt aacgatatct tcgggcgtcg gctcgaggtc gttcccgctc
     4441 gagtctccag cgttcgcact ccagggcgcc gccagcatgg ccgcccccgc accgagcgct
     4501 ttggcaaact tgcgccggtt cagatagacc tgctcaggtg tgatctccga gggtcggacg
     4561 tcgtcgggtc tgcggatcag cacgtcattt ccagaacagc cagattccca acgttagacc
     4621 ccaccggacg tcccggttcc cggcaaccgt tctcagcctt tcgacccgcg gcgggtcagc
     4681 cgaccccagg cccactgccc aggacccgaa agggcataga gcgagaagag gaacagcaag
     4741 accagtggcg ggtccgcgag ggtgacggcg aatatcagcg caaccagcac catggtgaca
     4801 aacggcaccc ggcctttcat gttcaacgcc ttcggcgagt agtaggtgaa attcgaaacc
     4861 atcagcagcc ccaaaaccac cgtgatggct cccatgccgg ctgccgccgc gcggcccgga
     4921 tcacctccga tgttgtcgct ccaggcccat accgagcagg cgatgatggc cgcagcagat
     4981 gggctggcca ggccggtaaa ggaactgacg tcgctcttga cgttgaaacg cgccaggcgc
     5041 agcgcggcac acgccatgta cacgaacgtc accacccagc cgatgcggcc cagcgcgtct
     5101 ccgagccccc atgagaacgc caccagcgcc ggcgccaccc cgaaagccac catgtcggaa
     5161 aggctgtcgt actcggcgcc gaactgactc tccgtacggg tacgtcgggc tacccggccg
     5221 tcggctgtat ccagcgccat ggcggcaaat atggcgaggg cggctgcagt gagatgttca
     5281 ttcatccccg ccacgatggc gtagaacccg gcaaagagag cgccggtggt gattagattc
     5341 ggcagcaggt agataccgcg catcctgttg gcagaggcgg ccccggagcc cgaccctgct
     5401 tcctcctcca cgcagtgaag cggcgggcgc tcctgattac cttcgctatc gctcaaatta
     5461 cccccttccg ggcgggcagc gtttgccgac aatcccgtgg cgagcgctgc gcttggagca
     5521 ccgcgtcgtt gcagcgcagg tcagttttta tccttgtcca ccagacgatt ctcctggatc
     5581 cagggcatca ttgcacgcag ccgctcaccc acctgctcga tctcgtgggc ctggctgata
     5641 cgccgcatgg ccttgatgcg gggcgctccc gcctggtttt ccgccacgaa ctcctgagcg
     5701 aatctgcccg tctggatctc ctcgagaatc ctgcgcatct cctgcttcgt ttcctcgttg
     5761 accaaccgcg gaccacgggt caggccgccg tactcggccg tgttggacac ggtgtaccac
     5821 atgttggtga taccgccttc gtagaagaaa tcgacgatga gcttcagctc gtggaggcat
     5881 tcgaaatagg ccatctcggg tgcgtagccg gcctccacca gggtttcgaa accggcctgg
     5941 accagggccg cagcgcctcc gcagagcacg gcctgctcgc caaagaggtc ggtctccgtt
     6001 tcttcctgaa acgtggtctc gatgattcca gcacgcccgc cgccgttggc tgacgcgtag
     6061 gacaacgccg tgttcttggc ctggccgctc gcgtcttgga aaatcgcgat gaggctcggc
     6121 accccgccgc cctcgacgta agtggagcgc accgtgtggc cgggtccttt cggcgcgatc
     6181 atgatgacgt cgagatcggc gcgcggctcg atgagctcga agtgaatgtt gaagccgtgg
     6241 gcgaaagcca gagtggcgcc ttgcttgacg ttgggcgcga tctggtcccg gtagatctgc
     6301 ggctggtgct cgtcgggcgt gagtatcatg atcaggtcgg cgtccttggc ggcgtcggcc
     6361 acgtccgcca cctgaaatcc cgcgttggtc gccttgcgcg cagaccccga atcgctgcgc
     6421 aggccgatgg tcacgttggc aacccccgag tcgcgcagat tgttggcatg agcgtggccc
     6481 tgggagccgt agcccaccac caggacgttc atcttctgga cgatcgacag atccgcgtcc
     6541 ttgtcgtagt aaacctgcat gtcgcttccc ccttttccag atccggattg caaattcttg
     6601 tccgccacga acatcagcga tccgcagacg ttcatggccc gccctagacg ctcagcacct
     6661 ggtcgccacg gctgacgccg gagacg
//
LOCUS       DBNHKV010000186         3403 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_379090, whole genome shotgun sequence.
ACCESSION   DBNHKV010000186 DBNHKV010000000
VERSION     DBNHKV010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_379090"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="AC16PP_05915"
     CDS             complement(<1..746)
                     /locus_tag="AC16PP_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6481200.1"
                     /translation="MSLSPYHHPTTICLVDDNQSFLDSMILEMPSMWACRAFTDPHAA
                     AEFLNQPIGLPPLAERCLSRETSTGSDAVIHIDLGLIEQEVNHPDRFRRISVLVADYA
                     MPSMNGLELCAAARDPHLRKAMLTGVADEKLAVEAFNEGLIHRFIPKHGTSATAIARE
                     YIAELQAEYFEQYTALLQGSLTVGKPGFLTDPDVSQWVEALMRTERLVEYYLVDDPPG
                     LLMLGSDGAMFRAMILDPVELSHQLEIARR"
     gene            complement(770..1066)
                     /locus_tag="AC16PP_05920"
     CDS             complement(770..1066)
                     /locus_tag="AC16PP_05920"
                     /inference="COORDINATES: protein motif:HMM:NF015807.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HIR6481201.1"
                     /translation="MKEKYYKVLVRLPPEMREMLAESARHYRRSRNSDIVARLQESFR
                     GIADSARGRGLAPPMHDQFEQLFRADLSPQEQSLVTAFRRLSSDQQTALLALLN"
     gene            1155..1910
                     /locus_tag="AC16PP_05925"
     CDS             1155..1910
                     /locus_tag="AC16PP_05925"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6481202.1"
                     /translation="MQPVRKDNIRLAATILLVRDVGGGPEVFMVQRPGGVDFPDLHVF
                     PGGKVDAADFVPELVTDLDDRSASQRLGVEAGGLRYWVAAIRECFEECGVLLADRDGE
                     LFTPRTDAEAERFAGHRHALISGELDFAAMCRQEGLAIRAGRAHYFSHWITPEVAPRR
                     FDTRFFIAHMPTGQVTAGHAWETAAEAWVAPGRALELFETGRWQMIAPTLTTLQTIAR
                     YSGVAHMLADVAAEAHLDELTADLRHQGMYPLR"
     gene            1921..2595
                     /locus_tag="AC16PP_05930"
     CDS             1921..2595
                     /locus_tag="AC16PP_05930"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6481203.1"
                     /translation="MNPVLSVAGLAKRYVDGSAERVVLAEVSLEVGPGEILAVCGPSG
                     SGKSTLLSIVAGLVLPDAGLIELTTPEGTLRIDGLSDAARADVRRRQLGYVFQFFNLV
                     PTLTVRENVLLPLELTGRGHLRAAALQRLADLGMDDRLDDFPENLSGGERQRTAIARA
                     LAHEPVLVLADEPTGNLDGGNANLVTDLLWREVRAQGAALLLATHNEAVAARADHVLR
                     LGAGAV"
     gene            2592..>3403
                     /locus_tag="AC16PP_05935"
     CDS             2592..>3403
                     /locus_tag="AC16PP_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6481204.1"
                     /translation="MSLLGLSQLRFLLRARWSTLSVLVGLTLGVAAVVAVHQIGSRIN
                     ASLEMVGLPHLDGLTELLDRDGANLTDADYFALRARWRAGEYTDVRALVPMVEGQRVM
                     AGRRALVVGVDWIAASSLPASGSGIDGRALIADGRLLVDGSFGLRAGESIEVGGASFQ
                     VEGVLDADLGGLVLADLGIAQTLLGLPPQALSQIGVRRSDPYARASELAERVLPGISA
                     GFARRSSGWQPQGWRARSISAEQPSLGFARSVVFNVGALGLLVLVVAWFLIY"
BASE COUNT          501 a         1056 c         1210 g          636 t
ORIGIN      
        1 taccggcgcg cgatctcgag ctgatggctc agttcaacgg ggtccaggat catcgctctg
       61 aacatcgcgc cgtcgctgcc gagcatcagc aggcccggcg ggtcatccac caggtagtac
      121 tcgaccagcc gctcggtgcg catgagtgcc tcgacccatt gcgagacgtc gggatcggtc
      181 agaaagccgg gcttgcccac ggtcaggctg ccctgaagca gggccgtgta ctgttcgaaa
      241 tattcggcct gcagctcggc gatgtattcc cgggcgatgg cggtcgccga cgtcccgtgt
      301 ttgggaatga accggtggat gagaccctcg ttgaaggcct cgactgccag cttttcatcc
      361 gcgacgccgg tgagcatggc cttgcgcagg tggggatccc tggctgcggc gcaaagctcc
      421 agcccgttca tggatggcat ggcgtaatcg gccaccagca ccgatatgcg cctgaaccgg
      481 tccggatgat tgacctcctg ctcgatgagg ccgaggtcga tgtgaatgac cgcgtcggag
      541 cccgttgagg tctcccggga aaggcagcgc tcagccagcg gcggcagccc gatgggttgg
      601 ttcaggaact ctgcagctgc gtgggggtcc gtgaacgccc ggcaagccca catgctgggc
      661 atctccagga tcatgctgtc cagaaaggat tgattgtcgt ccaccaggca gatcgtggtg
      721 gggtggtgat agggtgaaag tgacatcagg ggtgtcccgg tccgcagcgt cagttgagca
      781 gggcgagcag cgccgtctgc tggtcactgc tcagccggcg aaaggccgtg acgaggctct
      841 gttcctgcgg gctgaggtcg gccctgaaca gctgctcgaa ctggtcgtgc atgggtggcg
      901 caagtccccg tccccgggcg gaatctgcta tgcctctgaa ggactcctgc agacgcgcta
      961 caatgtcgga gttcctgctg cgccggtagt gtcgcgccga ctctgcgagc atctctcgca
     1021 tttccggcgg aagacgcacg aggaccttgt aatatttttc tttcattttg aggtctcggc
     1081 cgcgtggtcg gtctcagact agcgcggcat gccgagcccg acaagagatg gcaatttgat
     1141 atcagttcct gcaaatgcag cccgttagaa aagacaacat caggctcgcc gcgaccatcc
     1201 tgctcgtccg cgacgttggc ggcggccccg aggtgttcat ggttcagcgt cccggcggcg
     1261 tcgacttccc ggacctgcac gtctttccgg ggggcaaggt ggacgcggcc gacttcgtgc
     1321 ccgagcttgt caccgacctg gacgaccggt cggcgtcgca gcggttgggc gtggaggcgg
     1381 gcggcctgcg atactgggtg gcggccattc gcgagtgctt cgaggagtgc ggcgtgcttc
     1441 tggcggatcg cgacggtgag ctcttcaccc cgcgaacgga tgccgaagcg gagcgctttg
     1501 ccggacaccg tcacgcgctc atctccggcg agctggattt cgcggccatg tgccggcagg
     1561 aagggctcgc catccgggcc ggccgcgcgc actatttcag tcactggatc acgcctgagg
     1621 tggcgccgcg gcgcttcgac acgcggttct tcatcgccca tatgccaacg ggccaggtca
     1681 ccgctgggca cgcttgggaa acggcggccg aggcctgggt agcgccaggc agggccctgg
     1741 agcttttcga gacggggcga tggcagatga tcgcgcccac gctgacgacc ctgcagacca
     1801 tcgcgcgtta ttcaggcgtt gcgcacatgc tggccgatgt ggccgcggag gcgcacctcg
     1861 acgagctgac ggccgatctg cgccatcagg ggatgtatcc actgcgatga gggcagaatc
     1921 atgaacccgg tcctgtccgt tgccggcctt gccaaacgct acgttgacgg aagcgccgag
     1981 cgggttgtgc tcgcggaggt ctccttagag gtgggtccgg gagagattct ggccgtttgc
     2041 gggcccagcg gctccggcaa gtccactctg ctgagcatcg tcgccggact ggtgctgccg
     2101 gatgcgggtc tgatcgagct gaccaccccg gaaggtactc tgcggatcga cgggcttagc
     2161 gatgcggccc gtgcggacgt cagacgccgg cagctcggat acgtgtttca gttcttcaat
     2221 ctcgtgccga cgctcacggt tcgggaaaac gtgctgctgc cgctcgagct gaccgggcgc
     2281 ggccatctgc gggcggccgc cctgcagcgg ctggccgatc tggggatgga cgaccgcctc
     2341 gacgatttcc cggagaacct ctcaggcggt gagcgtcaac gcacggccat cgcccgggcg
     2401 ctcgcccacg agccggtgct ggttctggcg gatgagccga cgggcaacct ggacggcggc
     2461 aacgcgaatc tggtgacgga ccttctctgg cgcgaggtac gcgcccaggg cgccgccctg
     2521 ctgttggcga cacacaacga ggcggtcgcg gcgcgcgcgg accacgtcct gcggctgggc
     2581 gcgggcgcgg tgtgagcctg ctcgggctca gccagctgcg gtttctgctg cgggcgcggt
     2641 ggagcacgct ttcggtgctg gtggggctga ccctgggcgt tgccgcggtc gtggccgtcc
     2701 atcagatcgg ttcccgcatc aacgcctccc tggaaatggt cggcctgccc catctagacg
     2761 gcctcaccga gcttctcgat cgcgatggcg caaatctcac cgatgccgat tatttcgcgc
     2821 ttcgggcgcg ctggcgcgct ggcgagtata cggacgtgcg ggcgctggtt cccatggtgg
     2881 aaggtcagcg ggtgatggcg ggacgccgcg cgctcgtggt gggcgtcgac tggatcgcgg
     2941 cctcgagttt gccggcctcc ggttccggga tcgatggtcg ggcgctgatc gccgacggcc
     3001 gcctgctggt agacgggagc ttcggccttc gggcgggcga gtccatcgag gtgggcggcg
     3061 cgtcattcca ggtggagggc gtgctcgacg ccgacctcgg cgggctcgtg cttgccgatc
     3121 tgggcatcgc ccagacgctg ctgggattgc ctccccaggc gctgagccag atcggtgtcc
     3181 gccgatcgga cccctacgcg cgggccagcg agctggccga gcgcgtgctc ccgggaatct
     3241 ccgcggggtt tgcccgccgc tcgtcggggt ggcagccgca gggatggcgc gctcgctcga
     3301 tctcggcgga gcagccgtcc ctgggctttg cgcgatcggt ggttttcaac gtgggagcgc
     3361 tgggtctgct ggttctggtg gtggcgtggt ttctgatcta cca
//
LOCUS       DBNHKV010000187        11893 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1796613, whole genome shotgun sequence.
ACCESSION   DBNHKV010000187 DBNHKV010000000
VERSION     DBNHKV010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11893)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11893)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11893
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1796613"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="AC16PP_05940"
     CDS             complement(<1..272)
                     /locus_tag="AC16PP_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481205.1"
                     /translation="MPPIAGAEPVRQMASEGDSDTARNALLRHSVRHVAMMVGCSMAG
                     SRPSAIPLGGSRTVSRTTTAATMRGIPTTKNATRQPKNSFIQPPAR"
     gene            complement(503..1669)
                     /gene="solA"
                     /locus_tag="AC16PP_05945"
     CDS             complement(503..1669)
                     /gene="solA"
                     /locus_tag="AC16PP_05945"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HIR6481206.1"
                     /translation="MSYDVIVLGVGAMGAAALERLASRGARVLGIEQWSVPNSLGSSG
                     GDTRLIRKAYFEHPDYVPLLERAYEGWRRLEALSGLELLYPTGTVYVGPPDGPLIGGS
                     RMAAESHRLEFEPLAPAELHDRFPGFRLPDGCVSAFEPQAGFVLAGHGINAFAKLAIA
                     NGATLMSQTRVDGWRAESGGVVVSTSAGELEAGALVIAAGSWSSDICRLPRVALNVTR
                     QTLFWIWPERCAEFSLGRFPCWALEIAGHPGIYYGFPACVPNTARVGAKFAHHAPGET
                     CEPDGPKAPPSEAEYQATLRAVRSLLPGLRGSRIGSSICMYTNSRDGHFIVDRHPDHD
                     AVTVACGFSGHGFKFAPVMGELLADYALEGTSRLPAEFLRIGTRDLWHADEPKP"
     gene            1788..2861
                     /locus_tag="AC16PP_05950"
     CDS             1788..2861
                     /locus_tag="AC16PP_05950"
                     /inference="COORDINATES: protein motif:HMM:NF016214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR6481207.1"
                     /translation="MDPVSQAALGAVVARSVSHRQLGAGAVALGAAAGALPDIDVFFS
                     LDGDYFDQLVSHRGITHSLFFAPVVGPLFGWLAWRWTARRRNDPGEPAALRAWMLAMT
                     LAMLSHPLLDLLTPYGTQLLQPFSDARFAVAAMPIIDPIYTIVLLAGLVAGYRFTAAG
                     GRIAAATLLVSCAYLGYGWHLNEGAERFARAQLAAQGLPNVEVAAFPTILQIHHRRVV
                     ARTPDADRVGFLSMWDPCDIGWQREPRLPRSQVEAFLRTREGAIFDWFTMGWSHYAIQ
                     DGHMTATDLRYGSDENPRASVFTASVALDHLGRPSGNIAGGRFTPDANGRNLERLIAM
                     TYAPGCGTSPGRMAQTSLEAGAG"
     gene            2911..4263
                     /gene="nhaC"
                     /locus_tag="AC16PP_05955"
     CDS             2911..4263
                     /gene="nhaC"
                     /locus_tag="AC16PP_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HIR6481208.1"
                     /translation="MPSGPMDDDGLTMEQEAGRPNDPSNLDVVVAIVPTTVLLALSVA
                     LFGSDSSSGANQIALVIGAALATMVGVKNGHSWKVIEENIVGGISTAMPAIMILLSVG
                     SLIGVWMLSGTVPTMIYYGLLLLEPAVFYAASCLLCAVTALSIGSSWTVAGTLGIGLI
                     GVAAGLELSLPITAGAIISGAYFGDKLSPLSDTTNLAPAVTEIDIFTHIRHMTWTTLP
                     SFVFALGAFTAIGLMESTPETQTSLRVTLETLDEAFAINPLMLLPVALVVLLAGRKKP
                     AVACILAGVALGILMALVYQRPAIVRFADDASLGPTVAALKAIWIVLFDGYAGSTGEA
                     NLDELLSRGGMSSMLNTVWLILAAMTFGAAMEQAGVLRRLVHALASAARSTGNLIATT
                     LVTCIGVNVLAADQYMSIVLTGRMFRSEYRRRGLHEKNLARTLEDGGTITSWTSLVAR
                     "
     gene            complement(4608..5237)
                     /locus_tag="AC16PP_05960"
                     /pseudo
     CDS             complement(4608..5237)
                     /locus_tag="AC16PP_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
     gene            complement(5234..5983)
                     /locus_tag="AC16PP_05965"
     CDS             complement(5234..5983)
                     /locus_tag="AC16PP_05965"
                     /inference="COORDINATES: protein motif:HMM:NF021511.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HIR6481209.1"
                     /translation="MPQPRFADARHRQFALGLLGVYLLIWTSLAIKPLYRADWLLENV
                     LVAIAVPMLVATYHRLPLSRISYAMLFIFFCIHAVGSHYTYAEVPYDDWTRRLFGTSL
                     GEAAGWDRNHYDRFVHLMYGLLIVYPVRELFLRVADARGFWGYFFPLLVVMSSSLAYE
                     LLEWGAAFVFGGELGMAYLGTQGDVWDAHKDSLLATGGALAATLVIAAVHSSFDRDFG
                     RDWSQSLTVKNPDPLGEAAIGRYLNERENAP"
     gene            complement(6052..6822)
                     /locus_tag="AC16PP_05970"
     CDS             complement(6052..6822)
                     /locus_tag="AC16PP_05970"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR6481210.1"
                     /translation="MLLCLDVGNSQIYCGVYDGDRLTVQFRRTSTVRSSSDELGVFLR
                     GALRENGVSPDGITQIAICSVVPDVLYSLRSCCQKYFGLEPLIMRPGIKTGLKISYKD
                     PKEVGADRIADAIGAIKLYPNRNLIVVDFGTATTVCAISRDREFLGGNIVPGVRLAMD
                     ALEEKTAQLPSVEIFPVTSAIGRSTVESIQNGLYWSNVGMIKELVARVTEEAFPDDAP
                     VVIATGGFATLFDGLGLFDAIVSDLILIGLREAVRLNA"
     gene            complement(6837..7613)
                     /gene="panC"
                     /locus_tag="AC16PP_05975"
     CDS             complement(6837..7613)
                     /gene="panC"
                     /locus_tag="AC16PP_05975"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR6481211.1"
                     /translation="MKVLSSLGDWRAFRAGLVGEIGFVPTMGALHEGHAQLLQRSREA
                     NDHTVVSIYVNPTQFNNPADLDSYPGTLGEDLCRARELGVNAVLIPSYDELYPDDFRF
                     RVEEHEFSRELCGAHRPGHFTGVMTVVLKLLNLVRPRRAYFGEKDYQQYLLIRDMCDA
                     FFVDVEIVPCATVREEDGLALSSRNALLDEKGRARAGRLNRLLRDAPSDAAASRALEQ
                     AGFEVDYVVTKADRRFAAATLRCGEREVRLIDNVAIAQHG"
     gene            complement(7610..8398)
                     /gene="panB"
                     /locus_tag="AC16PP_05980"
     CDS             complement(7610..8398)
                     /gene="panB"
                     /locus_tag="AC16PP_05980"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR6481212.1"
                     /translation="MNVLDFPARKLNREKISMVTCYDFWSAQILEQTEVDCLLVGDSL
                     AMVMHGFDSTVHADTALMALHVAAVRRGAPSKFIVADMPFLAARKGLQPAMDAVQTLM
                     QAGANAVKIEGEAGQAELMTHIVQSGVPVMGHLGLTPQSVHTIGGHKVQGRTDEAAEQ
                     LRSSAEALAAAGCFALVLECVPSRLAAKVTRAIDIPTIGIGAGPETDGQVLVLQDLLG
                     MNPDFKPKFLRHYANGFESVSGAVNRFHADTQRGCFPSKSESYA"
     gene            complement(8687..10030)
                     /locus_tag="AC16PP_05985"
     CDS             complement(8687..10030)
                     /locus_tag="AC16PP_05985"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR6481213.1"
                     /translation="MMWNDSESEIFAQMAGRLAAAFADLPAFSAGLLDRDAVEVLRET
                     AQRLADNYPYFHPLYAGQMLKPPHPVARLAYGLAMLINPNNHALDGGRASSEMERAAV
                     AELAAMFGWQTHLGHLTSGGTMANLEALWIAGALQPGGVVLASEQAHYTHERLSGVLR
                     LPFEPIAADASGKMRMDALEDRLRRGGVGTVVATLGTTGFGAVDPLADIVELRHRYGF
                     RLHADAAYGGYFVLAGNLQAETRRHFDAMGAVDSIVVDPHKHGLQPYGCGSVLFRDPA
                     VGAFYKHDSPYTYFTSDALHLGEISLECSRAGAAAVALWATHRLLPPRPGGEFARGLE
                     RCRDAAEALHGRLAGHHRFLPLVQPELDIVAWAVLDADAEEASAAGRRLFEEAGELGL
                     HLALVQAPGSMIAHRWPEGADIPEGPVTCLRSVLMKPEHLDWLDRIWSLLEDLAG"
     gene            10276..11412
                     /locus_tag="AC16PP_05990"
     CDS             10276..11412
                     /locus_tag="AC16PP_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003116511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6481214.1"
                     /translation="MNYNLTEDQLAIKDLAERFTAEQITPNAAEWDEKHIFPRETIRA
                     AAELGFGGIYVSEASEGIGLGRIEAALIIEAMAYGCPSTSAFISIHNMAAWMIDQFGS
                     DDVRKRFVPDLVTMEKAASYCLTEPGAGSDAASLKTRAVRDGDHYVINGSKAFISGGG
                     ENEIYVCMLRTGDEGHRGISCIVVEKDTPGLSFGAQERKLGWHSQPTAMVNFEDVRVP
                     VSNRVGEEGEGFKIAMAGLDGGRLNIAACSIGGAQRCLDEAIGYVKERRQFGSAIADF
                     QNTQFMLADMATQLAAARALTHMAAQKVTENAADKTEFAAMAKLIATDSGSKIANDAL
                     QLHGGYGYLMDYPVERFVRDLRVHEILEGTNQIMRMVIGRSLVS"
     gene            complement(11471..>11893)
                     /locus_tag="AC16PP_05995"
     CDS             complement(11471..>11893)
                     /locus_tag="AC16PP_05995"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6481215.1"
                     /translation="LDVNLRGPFRLSALIGARMAEGDGGSIINVSSTAAVQPTPTELP
                     YAMAKAGINAFTLGLARAYAPKVRVNCIMPGPFLTDISKAWDMDAFNERAKTTIPLQR
                     GGEPDEIVGAALYFASAASSYTTGSVLKVDGGSAYPAA"
BASE COUNT         2272 a         3933 c         3663 g         2025 t
ORIGIN      
        1 tttctggcgg gcggctggat gaaggagttc ttcggctgac gggtcgcatt cttcgtcgtc
       61 gggatacccc gcatcgtagc ggctgtggtt gttcggctga ccgtccgcga accgcccagc
      121 ggtatggccg agggacgcga accggccatc gagcagccca ccatcatggc gacgtgccga
      181 acgctgtggc gaagaagggc attccgggcg gtatcggaat cgccctcgga ggccatctgg
      241 cggaccggct cggcgcccgc gatcggtggc ataagaaata ctgatttttg ttcttaatat
      301 cattaggtta ggcctataac ctaaaaccgt aggcattatc agcgatctcc tccaacctgc
      361 ctacgggccg gagtcgctcc gctacagcct tctcgtctgc tcgttcgtga atgtctgggc
      421 tgccgcccac tattacccgg cgggaacata cctcccaggc gacctggcgg ccgcagagcg
      481 cggcagcgac tgaccaccga gcctatggtt tcggttcatc ggcatgccac agatcgcggg
      541 tcccgatccg aaggaattcc gccggcagcc gcgaggtacc ctcgagcgcg tagtcggcca
      601 gcagctcgcc catcaccggc gcgaatttga atccgtggcc gctgaacccg caagccacgg
      661 tgaccgcgtc atgatcgggg tgccgatcga cgatgaaatg accatcgcgg gaattcgtgt
      721 acatgcagat cgacgagccg atgcgcgagc ctcgcagccc ggggaggaga gacctcacgg
      781 cgcgcagcgt cgcctgatac tcggcctcgg agggcggcgc tttcgggcca tcgggttcgc
      841 atgtctcgcc gggagcatga tgggcgaact tggctcccac acgcgcggta ttcgggacgc
      901 aggcagggaa cccgtagtag atccccggat gccccgcgat ctccagcgcc cagcacggaa
      961 acctgcccag agagaattct gcgcagcgct cgggccagat ccagaaaagc gtttggcggg
     1021 tcacgttcag agcgacgcgg ggcagccgac agatgtcgct cgaccagctt cccgccgcga
     1081 tgacgagtgc accggcctcg agctccccag cgctggtgct gaccactacc ccgcctgatt
     1141 cggcccgcca accgtctacc cgggtctggc tcatcagcgt agcgccgttg gcaatggcga
     1201 gcttcgcaaa cgcgttgatg ccgtgaccgg cgagcacgaa cccggcttgc ggctcgaagg
     1261 ccgagacgca gccgtcgggt aagcgaaacc ccgggaaccg atcgtgcagc tcggctggcg
     1321 cgagcggctc gaattccagt cgatgggatt ccgcagccat tcgtgagccc ccgatcagcg
     1381 gcccatccgg aggccctaca tagacggtgc ccgtcggata gagcagttcg agcccgctga
     1441 gagcctcgag tcggcgccag ccttcgtaag cccgctcgag tagcggaaca tagtcgggat
     1501 gctcgaagta agcctttcgg atgaggcgcg tatcaccccc actggagccc aaggagttgg
     1561 gcaccgacca ttgctcgatg cccagcaccc tggcgccccg ggatgccagc cgctccaaag
     1621 ccgcggcgcc catggcgccg acaccgagga cgatcacgtc gtagctcatg gcgcaatcct
     1681 atctcagctc ggcgaagcgg gcagagggaa cagtgcacat tgcggctgcc aatcggctcc
     1741 atcgccggta tgatgctccc aactgctcca gccgtcgtgt cacttccgtg gatcctgtct
     1801 cccaagccgc cctcggcgcc gtggtcgccc gcagtgtcag ccatcgccaa cttggtgccg
     1861 gcgccgtcgc gcttggggcc gcggcaggcg ccctgccgga catcgacgtg ttcttctccc
     1921 tggatggtga ctacttcgac cagctggtta gccatcgcgg gatcacgcat tcgctgtttt
     1981 tcgcccccgt ggtgggcccc ctcttcgggt ggctggcctg gcgctggacc gcccgacgcc
     2041 gaaacgaccc cggggagccg gcggcattgc gcgcctggat gctcgcgatg accctggcga
     2101 tgctttccca cccgcttcta gatttactaa cgccgtacgg cacgcaactc ctccagccct
     2161 tcagcgacgc gagattcgcg gtggcggcca tgcccatcat cgatccgatc tacaccatcg
     2221 tgttgctggc tgggctcgtt gccggatatc ggttcacggc ggccgggggg aggatcgcgg
     2281 cagcaacgct gctcgtctcc tgcgcttacc tgggttacgg ctggcacctc aacgagggcg
     2341 cggagcggtt tgcccgcgcc cagctcgcag cccaggggtt gccgaacgtc gaggtagccg
     2401 cgttcccgac gatcctgcag attcaccacc gacgtgtggt cgcacgcacc ccggacgccg
     2461 accgggtcgg gttcctctcc atgtgggatc cgtgcgatat cggctggcaa agggaacctc
     2521 gcctgccccg atcacaggtg gaggccttcc tccgcacgcg ggaaggggcg atattcgact
     2581 ggttcaccat gggttggtca cactacgcca tccaggacgg gcacatgacg gcaaccgatc
     2641 ttcgctacgg gtctgatgag aacccccggg caagcgtttt cacggcatcg gtcgccctgg
     2701 accacctcgg gcgcccctca ggaaatatcg ccggcggccg cttcaccccg gacgccaacg
     2761 gccggaacct cgaacggctg atcgccatga cctacgcacc cggctgcggc acgagccctg
     2821 gacggatggc ccaaacgagc ctggaagccg gcgccggctg atggcgtcaa cggagcatct
     2881 gcaacgatcg cttcgaacgc agtcagggcc atgccttccg gacccatgga tgacgacggt
     2941 ctgaccatgg agcaggaagc cggccggccg aacgacccaa gcaatctgga cgtcgtggtg
     3001 gccatcgtcc cgacgacggt cctcctggcg ctgtcggtcg ccctcttcgg cagtgactca
     3061 tcgagtggcg ccaatcagat tgccctggtc atcggagctg cgctggccac catggtcggc
     3121 gtgaagaacg gccattcctg gaaggtgatc gaagagaaca tcgttggcgg catttccacc
     3181 gcgatgccgg caatcatgat tctcctgtcg gtcggcagcc tcatcggggt gtggatgctg
     3241 tcaggaaccg tgcccacgat gatctattac gggttgctgc ttctcgaacc agcggtgttt
     3301 tacgccgcga gttgtctcct gtgcgccgtg acggcgctct ccatcggaag ctcctggacg
     3361 gtagcgggca cgctcggcat cggtctgatc ggggtagccg ccgggctcga actttcgctg
     3421 ccgatcaccg ctggggccat catctccgga gcctatttcg gcgataagct ctccccactt
     3481 tccgatacca cgaatctcgc cccggccgtg accgagatcg acatcttcac ccacatacgg
     3541 cacatgacct ggaccaccct gccgagcttc gtgtttgccc ttggggcgtt caccgccatc
     3601 ggcctgatgg aaagcacgcc cgagacgcaa acgtcactgc gcgtcaccct ggaaaccctc
     3661 gacgaagctt ttgccatcaa ccccctgatg ctgctccccg tcgccctcgt cgttcttctc
     3721 gcgggcagaa agaaaccggc cgttgcctgc atccttgccg gcgtggctct gggaatcctg
     3781 atggcgctcg tctaccaacg tcccgccatc gtccggtttg cagacgacgc gtccctgggg
     3841 cccacggtcg ccgcgctcaa ggcaatctgg atcgtcctgt tcgacggcta cgccggctcc
     3901 accggcgaag ccaatcttga cgagttgctc tctcgcggcg ggatgagcag catgctcaac
     3961 accgtctggc tgatactggc agcaatgacc ttcggagccg ccatggaaca ggcaggggtg
     4021 cttcgccggc tggtccatgc tctggcatcc gcagcgcgca gcacgggcaa tctcatcgcg
     4081 acgacactgg tcacctgcat cggggtgaac gtgctggccg ccgatcagta catgtccatc
     4141 gtcttgaccg gccgcatgtt cagatcggaa tatcgacgcc gcgggctgca cgagaaaaac
     4201 cttgcccgga ccctcgaaga cggcggcacc atcacctcct ggaccagcct ggttgcgcgc
     4261 tgatccggag gcacgctggc aacctgctcg aagttgacgg ctccaaccac tcccgcgggg
     4321 ccctgaagcg gccagagaat ctggttgctc ctgagcaggt agtcgttgat gaactcatcg
     4381 atcgtgatgc ccgacggtgc gaactcgaag cgagtgcgca tcacgtcgcg gacccgaagg
     4441 ggagcgagcg tctgctgagc gagggcctgg gagtaactgg ccgctgcgag atggctcaaa
     4501 aaccagccga tgagcagcag ccagagcccg ccagcggatt tgagcaccag caggttccac
     4561 aggccgatgc ccatgatggc ccagccgaac cagcgaccgg tggtacttgc cagccgtgtt
     4621 gccctgagct ggtttccggt cagcttccag ataatggcac gaaacaccct gccgccatca
     4681 agcgggaaac cgggaatcag gttgaacacg gccagcatca tgttcacgga gccgagccac
     4741 agacaggcgg tgtatcccgg gctcaggctg gcgatgacgg tttccggatc ttccgcaaac
     4801 gcttccctcg ccagggtgtc catgctccca aacgcaacca ccgtgaacac cgcgcccagc
     4861 agagcgctca tcaacggacc cgcaatggct atggtcagct ctgctgacgg ggtttgcgcc
     4921 tccgactcga tctcggctac gccgccaaac aggaacaggg taattctcgg cacccttatc
     4981 ccgtatcggc gggccgtgag cgaatgagaa agctcgtggg ccagcaacga tgcgaagaag
     5041 agcacgccgg ccaccagcgc cgtcagccac gccgtcgagg gcggccagtc gggatgccac
     5101 tggggaaaca gcccgcctcc caggctgtac acgacgaggc cgaagatgat ggcaacgctg
     5161 taatcgagcc ggatctcgat gccgaagatg ctgaacagct tcagccctcc gcccgcactc
     5221 ggcaacaatg gactcatggg gcattctccc gttcgttcag ataacgcccg atggctgcct
     5281 cgcccaaggg atcgggattc ttgacagtca ggctctgcga ccaatcccgg ccgaagtccc
     5341 ggtcgaagga tgagtggacg gcggcaatca ccagggtggc ggccagcgcg ccgccggttg
     5401 ccagaaggct gtccttgtgg gcgtcccaga cgtcaccctg ggtgccgaga taggccatac
     5461 ccaactcgcc gccgaacacg aaggcggccc cccactccag cagctcgtag gccagcgagg
     5521 acgacatgac caccagaagc gggaaaaaat atccccagaa acctctggca tccgcgacgc
     5581 gcaggaagag ctctcgtacg gggtaaacga tgagcagccc gtacatcagg tggacaaagc
     5641 gatcgtagtg gttgcgatcc caaccggctg cttcaccgag gctggtgccg aacagccggc
     5701 gggtccagtc gtcgtagggc acttcggcat aggtgtagtg cgaaccgacg gcgtgaatgc
     5761 agaagaagat gaaaagcatc gcgtacgaga ttctggacag aggcagcctg tgataggtcg
     5821 ccaccagcat tggcaccgcg atggccacca ggacgttttc cagaagccag tccgcccgat
     5881 acagcggctt gatggccagg gacgtccaga tgagcaggta aacgccaagc agtcccagtg
     5941 caaactgtcg gtgcctggcg tcagcaaatc ggggctgcgg catgctcata accgcgagca
     6001 taacaggggg gagtcataaa acgacgcatg aacgcctgca gggttttcag gtcaggcgtt
     6061 cagccggacc gcttcacgca ggccgatcag gatgagatcg gagacaatgg cgtcgaagag
     6121 ccccagcccg tcaaagagcg tcgcgaagcc ccccgtcgcg atcacgaccg gcgcatcgtc
     6181 cgggaacgct tcctcggtca ctcgggctac cagctccttg atcatgccga cattgctcca
     6241 gtaaagaccg ttctgaatgc tctccaccgt ggagcgcccg atagccgagg tcaccgggaa
     6301 tatctccacg ctaggcagct gcgcggtttt ctcttccagg gcatccattg ccagtcggac
     6361 gcccggcacg atgttgcctc cgagaaactc acggtcccgg gagatggcgc agacggtggt
     6421 cgcagtccca aaatccacca cgatgaggtt ccgattcgga tagagcttga tggcgccaat
     6481 ggcatcggcg atccggtccg cgcccacctc ctttggatcc ttgtaggaga tcttgaggcc
     6541 cgtcttgatg cccgggcgca tgatcagcgg ctcgaggcca aagtatttct ggcagcagga
     6601 gcgcagcgag tagagaacgt cgggcacgac ggagcagatg gcgatctgag tgatcccgtc
     6661 gggtgacacg ccgttttctc gcagcgcgcc gcgcagaaac actccgagct cgtccgagga
     6721 gctacgcacc gtagaggttc gtcgaaactg cacggtgagg cggtcgccat cgtagacgcc
     6781 gcagtagatc tggctgttac ccacatccaa acagagcagc atgcctcctc ctcggcctag
     6841 ccgtgctgcg cgatcgcaac gttgtcgatc agtcggactt ctctttcgcc gcagcgcaaa
     6901 gtggcggcgg cgaatcgccg atcggctttg gtgaccacgt agtccacctc gaatcccgcc
     6961 tgctcgagag cccgggaagc agcagcatcg ctcggcgcgt cgcgcagcag ccgattcagc
     7021 cgaccagcac gcgcgcggcc tttctcgtcc agcaacgcgt ttcgggaact cagcgccagc
     7081 ccgtcctcct cgcgtaccgt ggcgcatggc acgatttcga catccacgaa gaacgcatcg
     7141 cacatgtcac gtatcagaag atactgctga taatcctttt cgccgaagta cgcgcgccgc
     7201 ggccggacaa ggttgaggag cttgagcacc acggtcatca cgccagtgaa gtgccccgga
     7261 cgatgcgccc cgcaaagctc acggctgaac tcgtgttcct cgactcgaaa ccggaagtca
     7321 tcgggataga gctcgtcgta gctcggtatc agcaccgcgt tcacgccgag ctcccgggcg
     7381 cggcaaaggt cctcgccgag cgtcccaggg tagctgtcga gatcggcggg attgttgaac
     7441 tgagtggggt tcacatagat gctcacaacc gtgtggtcgt tcgcttcccg actgcgctgg
     7501 agcagctggg catggccttc atggagcgcg cccatggtcg gcacgaaccc gatctcgccg
     7561 acgagcccgg cgcggaacgc ccgccaatcc ccgaggctgg agaggacttt cacgcgtagg
     7621 actccgattt cgacggaaag cagccgcgct gggtgtcggc gtgaaaccgg ttgacggcgc
     7681 cgctcaccga ctcgaacccg ttggcgtaat gacgcagaaa cttgggcttg aaatcgggat
     7741 tcatgccgag cagatcctgc agaacgagca cctgcccatc cgtttccggt ccggctccaa
     7801 taccgatcgt cggaatgtcg atggcccggg tgacctttgc ggcgagccgc gagggcacgc
     7861 actcgagaac cagcgcaaag caaccggccg cggccagcgc ctcggcagag gaccgcagct
     7921 gctcggcggc ctcgtcggtc cggccctgca ccttgtgccc gccgatggtg tgtaccgact
     7981 gaggggtgag cccgaggtgt cccatgaccg gaacgcccga ctgcacgatg tgcgtcatca
     8041 gctcggcctg ccccgcttca ccctcgatct tcacggcgtt tgcgccggcc tgcatcagcg
     8101 tctggacggc gtccatcgcg ggctgcagcc ccttgcgggc ggcaagaaac ggcatgtcgg
     8161 cgacgatgaa tttcgacggc gcgccccgac gcacggcggc gacgtgcaac gccatcagcg
     8221 cggtatccgc atgcaccgtc gagtcgaatc cgtgcatgac catcgccagg ctgtcaccga
     8281 ccagcagaca atccacctcg gtctgctcca ggatctgagc agaccagaag tcataacagg
     8341 tgaccatcga gattttttct cgattaagtt tgcgcgcggg aaaatcaagc acattcactg
     8401 cggggccctc cggttgcgat caccgatgca gtacccgcac gagcggcgca cgccgttctg
     8461 cttcaggtac ctgtcctttc atcgggatca agtccaattg cctatggcgg ttcaaatctc
     8521 tgcctggcgc cgacgtcaat gcggggcgcc acggtctccg tgctgagcat cggagccgca
     8581 gaaaacccta cgtcactctc acaggaaagt ccatccgctc cggtgaacgc gcaacgggcg
     8641 caaaatgaga cccaaaaccc ctttctcctt ccgcgtgaat ggagtttcag ccggccagat
     8701 cctccagcag tgaccagatt cggtccagcc aatccagatg ttccggcttc atgagcaccg
     8761 accgcagaca ggtcaccggt ccctcgggaa tgtccgcgcc ctcgggccag cggtgagcaa
     8821 tcatcgaccc cggcgcctgg accagcgcca ggtgcagccc gagctcaccg gcttcctcaa
     8881 acaggcgccg gcccgccgcg ctggcctcct cggcatctgc atccaacacc gcccatgcga
     8941 cgatgtcgag ctcgggctga acgaggggca ggaacctgtg gtgaccggcg agtcggccgt
     9001 ggagagcttc agcggcgtcg cgacagcgct cgagcccacg ggcgaactcg ccacccggcc
     9061 tcgggggcag caaccgatgg gttgcccaca gcgcgacggc cgccgccccc gcccgcgagc
     9121 attccaggct tatctccccg agatgcagcg cgtcggaggt gaagtacgtg taaggcgagt
     9181 catgcttgta aaacgcgccc accgccggat cgcgaaacag cacgctgccg cagccgtaag
     9241 gctgcagtcc atgcttgtgc ggatccacca cgatggaatc gactgcaccc atggcatcga
     9301 aatgccgacg ggtctctgcc tgaaggttgc cggcgagaac gaaatagcca ccgtaagcgg
     9361 catcggcgtg cagccgaaac ccatagcggt gccgtaattc aacgatgtcg gcgagaggat
     9421 ctacggctcc gaagcccgtg gtgccaaggg tagcgaccac cgtacccacg ccaccgcggc
     9481 ggagccgatc ctccagcgca tccatccgca tcttacccga ggcatctgct gcgatcggct
     9541 cgaacggcag cctcagcacg ccgctcaggc gctcatgggt atagtgcgcc tgctccgagg
     9601 cgagcacgac cccaccgggc tgcagcgcgc cggcgatcca cagcgcttcc aggttggcca
     9661 tcgttccacc gctggtgaga tgtccgagat gggtctgcca accgaacatg gctgccagtt
     9721 ccgccacggc cgcccgctcc atctcggagc tggcgcggcc tccatccagc gcgtggttgt
     9781 tcggattgat gagcatggca agaccgtatg cgagccgggc caccggatgc ggcggcttca
     9841 gcatctgccc cgcgtacagc gggtggaagt acgggtagtt gtctgcgaga cgctgcgcgg
     9901 tctcccgcag aacctcgacc gcatcacggt cgaggagccc ggcgctgaag gctggaaggt
     9961 cggcaaatgc tgcagcgagt cgaccagcca tctgcgcgaa tatctccgac tcggagtcgt
    10021 tccacatcat cagcctagct ctccattgcc ctgctcatgc cgccggatca gcatgggcga
    10081 gaccacctaa ggatgagggc cctgagcgct cgatcccccg acagctgcct ctcatcgacc
    10141 tcggacgatc ctccctgcta gggatatcgc gcgcaacccc ggggcatctg aacggttgcg
    10201 cgtcgcatcg gtatctgagg gctgtgcaat gcattagaat cccccgctgc ccgcgacccg
    10261 acggatccaa acccgatgaa ctacaacctc accgaagatc aactcgccat caaggatctg
    10321 gccgaacgct tcacggcgga gcagattacg cccaacgcgg ccgagtggga cgaaaagcac
    10381 atcttcccgc gggaaaccat ccgcgcggcc gccgagctag ggttcggagg aatctacgta
    10441 tcggaagcct ccgaaggcat tggccttgga cgcatcgagg ccgccctcat catagaagca
    10501 atggcctacg gctgtccctc cacatcggct ttcatctcga tccacaacat ggccgcctgg
    10561 atgatcgacc agttcggctc cgacgacgtc agaaagcgct tcgttcccga cctggtaacc
    10621 atggagaagg cggcgagcta ctgcctcacc gaaccaggag ccgggtcgga cgccgcatcg
    10681 ctgaaaacga gagccgtgcg cgatggcgat cactacgtca tcaacggcag caaggccttc
    10741 atttcaggcg gtggcgagaa cgaaatttac gtgtgcatgc tgcgcacggg agacgaggga
    10801 catcgcggga tttcctgcat cgtcgtcgag aaggacacgc ccggcctgag cttcggcgcc
    10861 caggagcgga agctcggctg gcattctcag ccgacggcga tggtgaactt cgaggacgtg
    10921 cgagtgcctg tttccaaccg ggtgggcgaa gagggcgaag gctttaagat cgccatggcc
    10981 gggctggacg gcggccggct caacatcgcc gcctgctcga tcggtggcgc gcagcgctgc
    11041 ctcgacgagg ccattggcta cgtgaaagaa cgccgacagt tcggctccgc catcgccgac
    11101 tttcagaaca ctcagttcat gctcgccgac atggccaccc agctggcggc agcacgtgcg
    11161 ctaacccaca tggccgctca gaaggtgacg gagaacgcgg ccgacaagac cgagtttgcc
    11221 gcgatggcga agctgatcgc cacggattcg ggctccaaga tcgccaacga cgccctgcag
    11281 ctccacggcg ggtacggcta cctcatggac tatccggtcg agcgcttcgt ccgagatctc
    11341 cgcgtccatg agattctgga aggcaccaac cagatcatgc ggatggtgat cggacgatcg
    11401 ctggtgagct gacggccgcc aggccccgct gcggcaatcg ccaggtcccg caaccaaggc
    11461 ccgaccgcac tcaggctgca gggtatgcgc tgccaccgtc caccttgagt acggacccgg
    11521 tcgtgtagct ggaggcagcc gaggcgaagt acagcgcagc cccgacgatc tcatcgggct
    11581 ccccgccgcg ctgcagcgga atcgtcgtct ttgcgcgctc gttgaacgca tccatgtccc
    11641 acgctttgct gatgtccgtc agaaagggcc ccggcatgat gcagttgacc cgcaccttcg
    11701 gcgcgtacgc ccgggcgagg ccaagcgtga acgcgttgat ccccgccttg gccatggcgt
    11761 agggcaattc ggtcggcgtc ggctggacag cagcggtgct gctgacgttg atgatcgatc
    11821 cgccgtcgcc ctcggccatg cgcgcgccga tcagcgccga gagccggaaa gggcccctga
    11881 gattcacgtc gag
//
LOCUS       DBNHKV010000188         2942 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_734581, whole genome shotgun sequence.
ACCESSION   DBNHKV010000188 DBNHKV010000000
VERSION     DBNHKV010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_734581"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="AC16PP_06000"
     CDS             <1..870
                     /locus_tag="AC16PP_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008094299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6481216.1"
                     /translation="VSFSTTENPKPRLGAVRDDGIADLSGSDGIPDTMLGLLNAGDAA
                     MKAAHEAAEAAADTVPLDQVTLHPPVLTPGKILAIGLNYGDHIEESGMGKPKHQVWFN
                     KQHNCVNGPYGDIALPSVSEMLDYEAELVVVIGKRCKHVPRERAAEVIAGYCCGNDVS
                     VRDWQMRAQTMQIGKSFDTHGPTGPWLVTPDEVGDPHDLDIRAEVNGELRQNSNTKHL
                     IYDCYDAVAHLTQAFTLDPGDLLFMGTPAGVGAAMKPPQFLKAGDVVRVEIEKLGYLE
                     NRVVPEAAECVIE"
     gene            complement(872..1471)
                     /locus_tag="AC16PP_06005"
     CDS             complement(872..1471)
                     /locus_tag="AC16PP_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6481217.1"
                     /translation="MAQAQTHDHEIVSIYPFTDEEVDQLMNNSVECVLMWATKDGWPV
                     GVTHAFVWHDGKIWLTFAAHRHRTVAIRRDNRVSVNVSSCGYPPGASEELPGGAITFK
                     GTGEFFDDEETKKWFYTALSQKLNPNSPEGEQFFYNLLDSPLRTVLAVTPVKKIMYNG
                     KLAGGHMAGTVKEEDLGPRLGVDKERMNAERAKRGLDPR"
     gene            1648..2871
                     /locus_tag="AC16PP_06010"
     CDS             1648..2871
                     /locus_tag="AC16PP_06010"
                     /EC_number="5.4.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR6481218.1"
                     /translation="MSNLRRSTQLYERAKAVIPGGIPGIRAPENFVPGAYPILLAGGR
                     GGHVTDVDGNDYVDLLLGYGPVILGHAEQAVDSAAHERAAHGFCLDLPEPVMIELAEQ
                     LVSMVPSAEQALLLKTGSDATSAAIRIARTHTGRSVVLRCGYHGWHDWCLQGDPGVPS
                     GTTDTVIAFPYNDLDRLAALLTAHSGDVATIMLTPVGHEFDRQIEQPEPGFLEGVRSL
                     ADQHGVVLAFDEVRTGFRVHPGGAQGLYGVIPDLTALGKAMSNGYAVTALVGRRDVMQ
                     SARSTFISSTYFPNGMEMAAALETLKLIAEHDVLTGIAATGAALAGGLSEIVARHGLP
                     VSLSPYDEMPFLHFDPDLASGQPERRDAFYGALARSGVFAHPRHHGFLCWRHSADDVT
                     RVLEAVDAAAAALGS"
BASE COUNT          519 a         1003 c          937 g          483 t
ORIGIN      
        1 gttagttttt ccacgacaga gaatccgaaa ccccgactcg gcgccgtgcg cgacgacggg
       61 atagcggatc tgagcggttc ggacgggatc cccgacacga tgctaggtct cctgaatgcg
      121 ggggacgctg ccatgaaggc cgcacacgag gccgctgaag cggccgctga caccgtgccg
      181 ctcgatcagg tgacgctcca cccgccggtg ctgacaccgg gaaagatact cgccatcggg
      241 ctcaactacg gcgaccacat cgaggagagc ggcatgggca aacccaagca tcaggtgtgg
      301 ttcaacaagc agcacaactg cgtcaacgga ccctatggcg acatcgcgct gccgtcggtc
      361 tccgagatgc tcgactacga ggccgagctg gtggtggtga tcggcaagcg ctgcaagcac
      421 gtgccgcgag agcgcgcggc ggaagtcatc gccggctact gctgcggcaa cgacgtgagc
      481 gttcgcgact ggcagatgcg cgcccagacc atgcagatcg gtaagtcatt cgacacccac
      541 ggccccaccg ggccatggct ggtcacgccg gatgaggtgg gcgaccccca cgacctggac
      601 atccgcgccg aggtcaacgg ggaactccgc cagaactcga atacgaagca cctgatctac
      661 gactgttacg acgccgtagc gcatctgacc caggcgttca ccctggaccc gggcgacctg
      721 ctgttcatgg gcacgccggc cggtgtcggg gcggcgatga agcccccgca gtttctcaag
      781 gccggagacg tggtcagggt ggaaatcgag aagctcggct acctcgaaaa ccgggtggtc
      841 cccgaagcag ccgagtgcgt gatcgagtga cctagcgggg atcgagcccg cgcttggcgc
      901 gctcggcgtt catgcgctcc ttgtcgacgc cgagccgcgg gccgaggtct tcctctttga
      961 ccgtcccggc catgtgcccg ccggccagct tgccgttgta catgatcttc ttcaccggcg
     1021 tgacggcaag cacggtccgc aacggcgagt cgagcaggtt gtagaagaac tgctcgccct
     1081 ccgggctgtt gggattgagc ttctgcgaca gcgcggtgta gaaccacttc ttggtttcct
     1141 cgtcgtcgaa gaactctccg gtgcccttga aggtgatggc accgcccggc agctcctctg
     1201 aagcgcccgg cgggtagccg cagctgctga cgttgacgga aacgcggtta tcgcggcgga
     1261 tggcaaccgt tctatggcga tgcgcggcaa aggtcagcca gatcttgccg tcgtgccaca
     1321 cgaacgcgtg ggtgacccct accggccagc cgtccttggt tgcccacatg agcacgcatt
     1381 cgacggagtt gttcatcagc tggtcgactt cctcgtccgt aaacgggtaa atcgagacga
     1441 tctcgtgatc gtgagtttgc gcttgagcca taagttcccc ctggagtaac tgccgtcccc
     1501 gatcccgggg aaaaagagcg gctagaataa tgcaatcgct cgatcggcga taccgacgag
     1561 ccccaccggc ctgctcgcgc cgatcggaaa cagcaacgtc attccgctta aacttcaacc
     1621 ggtaaaggag catccacggt aacgaccatg agcaacctgc gccgatcgac ccaactctac
     1681 gaacgcgcca aggccgtcat tcccgggggc atccccggca tccgggcgcc ggaaaatttc
     1741 gtcccgggtg cttaccccat acttctggcc ggaggccgcg gcggtcacgt cacggacgtg
     1801 gacggcaacg actatgtgga cttgctgctg gggtacggac ccgtgatcct cgggcacgcc
     1861 gagcaggccg tggacagcgc ggcccacgaa cgcgccgccc acggcttctg cctggatctc
     1921 cccgaacccg tcatgatcga gctggccgag cagctcgtat ccatggtgcc ctctgcagag
     1981 caggcgctgc tgctgaagac gggctccgat gccacgagcg ctgcgatccg catcgccagg
     2041 acccacacgg gcaggtccgt ggtcctgcgc tgcgggtacc acggctggca cgactggtgt
     2101 cttcagggag atcccggcgt gccgtccggc accaccgaca ccgtcatcgc gttcccctac
     2161 aacgacctcg accgcctggc cgctctgctg acggcccact cgggcgacgt ggcgaccatc
     2221 atgctcaccc cggtgggtca cgagttcgac cgccagatcg agcaacccga accaggtttt
     2281 ctggaggggg tgcgcagcct ggccgaccag cacggcgtgg tgctcgcctt cgacgaggtt
     2341 cgcacgggct tccgcgtcca cccgggcggc gcccagggcc tctacggcgt catccccgac
     2401 ctgaccgctc tgggcaaggc catgtcgaac ggttacgcgg tgacggccct ggtcggccgg
     2461 cgggacgtca tgcagagcgc ccggagcacg ttcatctcat ccacctactt ccccaacggc
     2521 atggagatgg cggcggctct cgagaccctg aagctgatcg ccgagcacga cgtgcttacc
     2581 ggcatcgccg ctaccggcgc tgcgctcgcc ggagggctgt cggaaatcgt cgcccggcac
     2641 ggcctgcccg tctccctgtc accctacgac gagatgccct tcctccattt cgacccggac
     2701 ctggcatcag gccagcccga gcgacgagac gctttctacg gcgccctggc ccggagcggc
     2761 gtattcgccc acccgcgcca tcacggattt ctctgctggc gccacagcgc cgacgacgtg
     2821 acgcgcgtgc tggaggcggt ggatgccgcc gcggcggcgc tgggaagcta gtccggcccc
     2881 cctggcgggc gggcgatgcc cgaccagttt cccgccagcc ggtcgtccgc cgccaggcgg
     2941 ac
//
LOCUS       DBNHKV010000189         3748 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1230512, whole genome shotgun sequence.
ACCESSION   DBNHKV010000189 DBNHKV010000000
VERSION     DBNHKV010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1230512"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..733
                     /gene="lptC"
                     /locus_tag="AC16PP_06015"
     CDS             104..733
                     /gene="lptC"
                     /locus_tag="AC16PP_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIR6481219.1"
                     /translation="MRKRSKWERWSTWTAILGVAVAGVVLLSEPLEPETEAETPPAGI
                     AEEPDLYVEDAVITQYLPSGTVHYRLVSKLLRQFEADELVRLTKPNLVVNSETRSPWQ
                     VQSEHGYVRRMGGPERGGEGEEVVFLREDVQLEQREPDGRHIRLTTETLYVYPEREYA
                     ETDRDVMIDTHAGRTTATGLKGDLQRGLLQLSSSDRRRVHTILLPDQFK"
     gene            747..1229
                     /gene="lptA"
                     /locus_tag="AC16PP_06020"
     CDS             747..1229
                     /gene="lptA"
                     /locus_tag="AC16PP_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HIR6481220.1"
                     /translation="MTLGAVLLAPGALALPEDADQPIHIRANSAEMDQEQGLVIYRGD
                     VQVDQGTLRVMADEMTVEYEDQEVIRITAKGEPARYQQQIEVDEEVVRARSSTIIYHT
                     RDERIDLVGNAFLTQEGNEITGDLIEYDIVAGKVDAKAESETPVRMILQPARKKPDGG
                     "
     gene            1238..1963
                     /gene="lptB"
                     /locus_tag="AC16PP_06025"
     CDS             1238..1963
                     /gene="lptB"
                     /locus_tag="AC16PP_06025"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003313594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIR6481221.1"
                     /translation="MPTLEVRSLRKQYRNKVAVEDVSLHVSTGEIIGLLGPNGAGKTT
                     TFYMIVGLLRADGGDIFLDEQRITRTPMHVRARAGIGYLPQEASVFRKLSTEDNVRAI
                     LELRRDLNRTQRQAMLDQLIEEFHLGGVRESLGERLSGGERRRLEIARALAMEPSFML
                     LDEPFAGVDPISVGDIKKEIRDLAARGIGVLITDHNVRDTLDICDRAYIVHEGHIIAE
                     GDSGAILADEKVKNVYLGESFSL"
     gene            2124..3599
                     /locus_tag="AC16PP_06030"
     CDS             2124..3599
                     /locus_tag="AC16PP_06030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR6481222.1"
                     /translation="MKQTLSLKLGQQLTMTPQLQQAIRLLQLSCLDLRQEIQDALEGN
                     PMLELDEEDGGDEPTGSPAEADRAESDGDDDLDGVDATLEGDVIDDLPVDTSWDEIYQ
                     PAPAPAPAAGDSESWDFETRNAPEETLQDHLLWQLNLTPMSDSDRVIAMAIIDTISPD
                     GMLTTPVEELVTSLEGVVDEEAEIAHAEVMAVLKQIQHFDPIGVGARDLGECLRIQLE
                     QLPAGTPWLAESLTLVNEHLPLLASRDFAALKRRTKLGEEELGEVVRLIQTLNPRPGT
                     TVAQEAAEYVVPDVMVSRKEGRWLVELNPDATPGLRINAGYAGLVKRADNSADNTFLR
                     DHLQEARWFLKSLQSRNETLLKVATRIVEHQRGFLEYGEEAMKPLVLNEIAEAVEMHE
                     STISRVTSRKYMHTPRGIFELKYFFSSHVSTSSGGEVSSTAIRALIKKLTADENPRKP
                     LSDSRIATILAEQNINVARRTVAKYRESMAIPPSNERKRLV"
     gene            3656..>3748
                     /locus_tag="AC16PP_06035"
     CDS             3656..>3748
                     /locus_tag="AC16PP_06035"
                     /inference="COORDINATES: protein motif:HMM:NF014534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HIR6481223.1"
                     /translation="MQLSISGHHIDVTEALRNYVTEKMQKLERHF"
BASE COUNT          791 a         1196 c         1190 g          571 t
ORIGIN      
        1 actacgtcac cctggccccc gggggtgcag gggcggcgcg ggagatcgcc cagcttttcc
       61 tggaagcccg gggcgagtgg ccctacgggt aaccggctga gccatgcgca agcgctccaa
      121 atgggaacga tggtcgacgt ggacggccat cctgggtgtt gcggtggccg gggtcgttct
      181 gctgagcgag cccctggagc cggagacaga agcggagacc ccgcccgccg gcatcgccga
      241 agagccggat ctttacgtcg aagacgccgt catcacccag tacctgccaa gcggaaccgt
      301 ccactaccgg ctggtgtcga aattgctgag gcagttcgag gcggacgagc tggtgcggct
      361 gaccaagccg aacctggtgg tcaacagcga aacgcgctcg ccgtggcaag tgcagtcgga
      421 gcacggctac gtgcgccgaa tgggcggacc cgagcgtggc ggcgaaggcg aagaggtggt
      481 ttttctgcgc gaggacgtcc agctagagca gcgggaaccc gacggtcgcc acatacgcct
      541 gaccacggag accctctatg tgtatccgga gcgagagtac gccgagactg accgcgatgt
      601 tatgatcgac acccatgccg gccggaccac ggccaccgga ctcaaaggtg acttgcagcg
      661 tggattgcta cagctgtcct cctcagatcg acgccgcgtg cacacgattc tgctgccgga
      721 tcagttcaag taggctgctg cgtgccgtga cgcttggcgc cgtcctgctc gctccgggcg
      781 cgctcgctct gcccgaggac gccgaccagc ccattcacat acgcgccaac agcgcggaga
      841 tggatcagga acaggggctg gtgatctatc gcggcgacgt tcaggtggac caggggactc
      901 tgcgcgtcat ggccgacgag atgaccgtgg agtacgagga tcaggaggtg atacgcatca
      961 ccgccaaggg agagcccgcc cgctaccagc agcagatcga ggttgacgag gaagtggttc
     1021 gcgctcggtc gtcgaccatc atctaccaca cgcgcgacga gcgcatcgac ctggtcggca
     1081 acgcgtttct cacccaggaa ggcaacgaga tcaccggtga cctcatcgag tacgacatcg
     1141 tcgccggcaa ggtggatgcg aaggcggaaa gcgaaacgcc ggtgcggatg atccttcagc
     1201 ccgctcgcaa gaaacccgac ggcgggtaaa cggccccatg cccacactgg aagttcgctc
     1261 tctgcgcaaa cagtaccgaa acaaggtcgc ggtggaagac gtcagcctgc acgtcagcac
     1321 cggcgagatc atcggcctgc tgggacccaa cggtgccggc aaaaccacta ccttctacat
     1381 gatcgtcggc ctgctgcgag ccgacggcgg cgatatcttt ctcgacgagc agcggatcac
     1441 ccggaccccc atgcacgtgc gcgcccgggc gggtatcggc tatctgcccc aggaagcaag
     1501 cgtcttcaga aagctcagca cggaggacaa cgtgcgggcc atcctggagc tgagacggga
     1561 cctgaaccgg acccaacgcc aggccatgct cgatcagctg atcgaggagt ttcacctggg
     1621 cggggtgcgc gagtccctcg gcgagcggct ctccggcggc gagcgacgcc ggctggagat
     1681 tgcccgggca ctcgccatgg agcccagctt catgctgctc gacgagccgt tcgcgggcgt
     1741 cgaccccatt tccgtcggcg acatcaaaaa ggagatccgc gatctggcgg cccgcggcat
     1801 cggcgtgctc atcaccgatc acaacgtgcg ggacaccctg gacatttgtg accgggccta
     1861 catcgttcat gagggccaca tcattgcaga aggcgattct ggcgccatac ttgcagacga
     1921 gaaggtcaaa aacgtatatc tgggcgagag cttctctctt tgagcctggc tccttgagat
     1981 cagccctcag ttccaggccg cgatagcgcg gccgcacagc caggggcgag cgtcaggcac
     2041 gccaagcctt ttgacccgtt aggacaacaa ccggcaacca tgccgcgcgg aaggacgacc
     2101 gctcaccaga gccctcggtt tgcatgaaac agactctgtc gctcaagctc ggacagcagc
     2161 tcaccatgac gccgcagctg cagcaggcca tccggctgct gcagctttcg tgcctggacc
     2221 tgcgtcagga aattcaggat gccctggaag gcaacccgat gctcgaactc gacgaggagg
     2281 acgggggcga cgagccgacg ggctctcccg cagaggccga tcgggccgaa agcgacggtg
     2341 acgacgacct cgacggcgtg gatgcgaccc tggaagggga cgttatcgac gatctgccgg
     2401 tcgacacgag ctgggacgag atctaccagc ccgcgcctgc tcccgctccg gcagccggcg
     2461 attccgaatc ctgggatttc gaaacgcgga acgcgcccga ggagaccctg caggaccatc
     2521 tgctctggca gctgaacctc acgcccatgt ccgactccga tcgggtgatc gccatggcga
     2581 tcatcgacac catcagtccg gacgggatgc tgaccacgcc cgtggaagag ctggtgacgt
     2641 ctctggaagg cgtggtggac gaggaggcgg agattgccca cgccgaggtc atggccgtgc
     2701 tcaagcagat ccagcatttc gaccccatcg gggtgggcgc ccgggacctg ggcgagtgcc
     2761 tgaggatcca gctcgagcag ctgccggcgg gcacgccgtg gctcgcggag tcgctgaccc
     2821 tcgtcaacga gcacctgcct ctgctggcca gccgggactt cgccgccctc aaacgtcgga
     2881 ccaagctcgg cgaagaggaa ctcggcgagg tcgtgaggct cattcagacg ctcaatccgc
     2941 gcccgggcac cacggtggcc caggaggcgg cagagtacgt cgtccccgac gtcatggtat
     3001 ccaggaaaga ggggcgctgg ctggtggagc tcaaccccga cgcgacgccg ggcttgagaa
     3061 tcaacgccgg ctacgccggg ctcgtcaagc gcgccgacaa cagcgccgac aacaccttcc
     3121 tgcgcgatca tctgcaggaa gcgcgatggt tcctcaagag cctgcagagc cgcaacgaga
     3181 ccctgctcaa ggtcgccacc cgcatcgtgg aacatcagcg gggattcctg gaatacgggg
     3241 aagaagccat gaagcccctg gtgctcaacg agatcgccga ggccgtggaa atgcacgaat
     3301 cgaccatctc ccgggtcact agccgcaagt acatgcacac gccccggggc atcttcgagc
     3361 tgaaatattt cttttccagc cacgtgtcca cgagcagcgg cggcgaggtt tccagcacgg
     3421 ccatccgcgc gctcatcaag aagctgacgg ccgatgaaaa tccaagaaaa ccgttgagcg
     3481 acagccgcat cgctacgata ctggcggagc agaacatcaa cgtcgccaga cgcaccgtgg
     3541 ccaaatatcg agagtcgatg gccataccac cgtccaacga acgcaagcgt ctggtttgat
     3601 cattcatgac ggcacacgac gcgactcgac atcaacagca aaacaaggag ccagcatgca
     3661 gttgagcatc tccggtcatc acatcgacgt caccgaggcg ctgcggaact acgtgaccga
     3721 aaaaatgcag aaactggaac gccacttc
//
LOCUS       DBNHKV010000190         2252 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_25097, whole genome shotgun sequence.
ACCESSION   DBNHKV010000190 DBNHKV010000000
VERSION     DBNHKV010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_25097"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="AC16PP_06040"
     CDS             <1..789
                     /locus_tag="AC16PP_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR6481224.1"
                     /translation="LFSWLWLGHPEHILPGVIMWSTLLLASVFATSVLSGVLGMAGGM
                     ILMAILVSLMSVPAAMIIHGVVQATSNGSRAWFLRAHIRWRVLPPYLLGAAVTLAAFA
                     ALTLVPEAGLVLVLVGIFPWLARVVPRLQGLDVERPATAVACGATVTAAQLFAGASGP
                     LLDVFYLNSSMDRYQVIASKAFTQTIGHGLKLAYYGTIIGVAETLPWWMFGAAMATAV
                     AGTRVGTRLLGRLRDDVFRRVSGWVILAIAAVCVVKGGMELAGW"
     gene            complement(808..>2252)
                     /locus_tag="AC16PP_06045"
     CDS             complement(808..>2252)
                     /locus_tag="AC16PP_06045"
                     /inference="COORDINATES: protein motif:HMM:NF014805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6481225.1"
                     /translation="RSHGINFIGPPVASMETMGNKSNAINTALRLNVPVVPGSHGILT
                     NVERAAEVAEEIGYPVLIKAVHGGGGKGIQVVESRDSFHELFHRVTVEARAAFGNGDV
                     YLEKYVTSLRHIEAQLLRDVHGNTRVLGIRDCSVQRDKQKVIEESDSTVLPDRLREEV
                     LQHTAALADEVGYVGAGTVEFIYDLKSDAVYFMEMNTRLQVEHPVTEWVSGVDIVSQQ
                     FRIAAGESIADLDPSPRGYSIEARVTAERVQAAPGGELVFRPQPGQVAECSFPEDEGV
                     EIIAAAAPGKFISPYYDSMIAQVVVYADDRAQAIEKLAAYLRRVRVTGICTNIPLLKH
                     ILDDEVFQQGVYDTGYLPGLLARLDADELIAEIEGGAGEVSGGIDMNAIRIDESDELK
                     VLSPATAIFYGTPTPTEPPYVSVGDRVKLSDTLCQLEAMKIFTPLSLADFNSEFELYE
                     AGREYEITRINMGTGQQVNAGDLLFVVRPV"
BASE COUNT          358 a          730 c          720 g          444 t
ORIGIN      
        1 cttttttcat ggttatggtt ggggcaccct gagcacattt tgccgggcgt gatcatgtgg
       61 tcgacccttc tgctcgcgag cgtcttcgca accagcgtgc tctccggcgt gctggggatg
      121 gcgggcggca tgatcctgat ggccatcctg gtctccctga tgtccgtgcc cgccgccatg
      181 atcattcacg gcgtggttca ggccacctcg aacgggtcca gggcctggtt tctgcgcgca
      241 cacatccggt ggcgggtgct gcccccttat ctgctgggcg cggccgtcac ccttgccgct
      301 tttgcagccc tgacgcttgt ccccgaagca ggcctggtgc tcgttctcgt cgggattttt
      361 ccctggctgg ctcgcgtcgt cccgcgactc cagggcctgg acgtggaaag gccggcgacg
      421 gcggtggcat gcggtgcaac ggttaccgcg gcgcaactat tcgccggggc atcgggaccg
      481 ctgctggacg tcttctacct gaactcgtcc atggaccgct accaggtgat cgccagcaag
      541 gcgttcactc agaccatcgg ccacgggctg aagctcgctt actacgggac catcatcggc
      601 gttgccgaga ccctgccctg gtggatgttc ggcgccgcca tggcgacggc cgtggccggc
      661 acgcgcgtcg gaacccggct gctgggccgc ctgcgagatg atgtgttccg ccgcgtgagt
      721 ggatgggtga tcctggcaat cgccgccgtc tgcgtcgtca agggcgggat ggaactggcc
      781 ggctggtgag cgctgcccgt gccctcccta aacgggccgc accacgaaca gcagatctcc
      841 cgcgttcacc tgttgtccgg tgcccatgtt gattcgggtg atctcgtact cccggcccgc
      901 ttcatagagt tcgaactcgg agttgaaatc ggccagcgaa aggggcgtga atatcttcat
      961 ggcttcgagc tgacagaggg tgtccgacag ctttacccgg tccccaaccg acacgtaggg
     1021 cggctcggtg ggcgtgggcg ttccgtagaa aatggccgtc gcgggcgaga gcaccttgag
     1081 ctcgtcgctt tcgtcgatgc gtatggcatt catgtcgatg ccgccggaaa cctccccggc
     1141 accgccctcg atttccgcga tgagctcgtc ggcatccagg cgggcgagaa gccccggcag
     1201 gtagcccgtg tcatacacgc cctgctgaaa gacctcgtca tcgaggatgt gcttgagcag
     1261 gggaatgttg gtgcagatcc ccgtaaccct gacgcggcgc agataggccg ccagcttctc
     1321 aatggcctgg gcacggtcat ccgcgtagac gaccacctga gcaatcatgc tgtcgtagta
     1381 gggggagatg aacttgccgg gcgctgcagc ggcgatgatc tcgacccctt cgtcctccgg
     1441 aaagctgcac tcggccacct gcccgggctg cgggcggaag accagctcgc cgccgggagc
     1501 ggcctggacg cgctcggccg tcacgcgcgc ctcgatggag tacccgcggg gtgacggatc
     1561 gagatccgct atggattccc cggcggcaat gcgaaactgc tgggaaacga tgtcgacgcc
     1621 ggagacccac tccgtcaccg gatgctcaac ctgcaaccga gtgttcatct ccatgaaata
     1681 cacggcatcg gacttcaggt cgtagatgaa ctcgacggtg cctgcaccca cataccccac
     1741 ttcgtccgcc agggccgccg tgtgctgcag gacctcctcg cgaaggcgat cgggcaacac
     1801 cgtggagtcg gactcctcga tcaccttctg cttgtcgcgc tgaacgctgc agtcgcggat
     1861 gccgagcacg cgggtattgc cgtgcacgtc cctgagcagc tgggcctcga tgtgccttag
     1921 agaggtgacg tacttctcca ggtaaacatc gccgtttcca aacgccgcgc gcgcttctac
     1981 cgtgacccgg tggaagagct cgtggaaaga atcgcgcgac tccaccacct ggatgccctt
     2041 accgcctccg ccatgcacgg ccttgatgag cacggggtat ccgatctcct ccgccacctc
     2101 ggccgccctc tcgacgttgg tgaggatccc gtggcttccg gggaccaccg gcacgtttag
     2161 gcgcagcgcg gtgttgatgg cattggactt gttgcccatg gtctccatgc tggcgaccgg
     2221 cgggccgatg aagttgatac cgtggctgcg gc
//
LOCUS       DBNHKV010000191         8067 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_948202, whole genome shotgun sequence.
ACCESSION   DBNHKV010000191 DBNHKV010000000
VERSION     DBNHKV010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8067)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_948202"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="AC16PP_06050"
     CDS             <1..1268
                     /locus_tag="AC16PP_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR6481226.1"
                     /translation="RYGTRTWAELVAPAVDLARNGFTVYPALAQYAREALTDLAGTNF
                     AAHFAALTTTRRFRQPDLADTLQRIAENGADEFYRGRTARLLAAEMARGGGLITREDL
                     AGYRAIWRDPIDFRWRDYRVLTAPPPSSGGVAIAQLLKMYEARGGDFADLGHNSAAYV
                     HLNAELAKRVFADRAVYLGDPDFVSVPVDSLIDDDYLRARASEANPAQITRPRPVPVI
                     AEHNDTTHFSIVDGAGNAIANTYTINTSFGSGVVVTGAGFLMNNEMDDFAVAPNSPNY
                     FGLVGSEANAIAPGKRMLSSMSPTIALRDGKVALVVGAMGGSAIITSVYQVMLNLEAF
                     GMEAPEALAATRVHHQFPPDDLITYDPSGALPTEAVAGLEALGYRVEPHSWAFGNVQL
                     IWRNREGVLQAASDERFSGASEVLRTPPP"
     gene            complement(1281..3653)
                     /locus_tag="AC16PP_06055"
     CDS             complement(1281..3653)
                     /locus_tag="AC16PP_06055"
                     /inference="COORDINATES: protein motif:HMM:NF017197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HIR6481227.1"
                     /translation="MQTLPVSVSRKIWIPAVIALLLISIGLAAPLAIKRYVEGWLRDR
                     GVAEARITDVDLNPFTGRVRVEYLDAPGAADESGSFEMLELDLELLGLITGRVMVSRA
                     ILRDAVLDVRRMPDGRFSVGGLMFPLAQAEQEVESDGEGPAGSPRPRLGLAGLLVENV
                     TLTYDDGMLSDRLYIRSLTIDRMVTWEPERAARLVAELELNGAPVGLEAEVFAFADER
                     RATISIDVDAFDVAAFAPLVADLGIESPGGSLSLSLGGQLRREASGRLFGNVAGTVGV
                     DDIAFGLHPLQIDLGKLSLDVDLAGEIEIQSGDSPPAISGQIGVAASDLLVDHRDWPA
                     RLLGLETMEAPSVRVDGLERASSDTLSVRGLRLLEEEEVVGLSVGSASVEGIELDTSA
                     RALRIGEVSLDDLVARLRRGEQGRLRLLGPAAAEEPSAQPEAAPGADDGAPSFELHVG
                     AVSIGGNSALGLEDLSVEPQVTMELVIDRFRLADLGIGPAAGPMVLDVALTQNGAQLS
                     ATGDLALFADARQVKADLEVVHFDLSQLTPYLPNYRVDRGRLSIQTQLGINGDEVAAE
                     NAVLLEKLKVGVKRSGDPGLVEEGLGMPLDVALDLLRDGDDRIKFDLPISGSIHDPKF
                     GTGDIVRLAMQNALQRAALSVATNALQPLGTIMFVGKMAGKAARPRFDPVAFEPGARS
                     LSELGTSYLDKLAGLLLERPRLHLTFCGVAAVQDVAAAEAVEGDGSASADGAPAVVDE
                     AALLELARARGSAVHDYLIDVRGIPAARLFRCHPEYEAESEEGPRVEIML"
     gene            complement(3658..4866)
                     /locus_tag="AC16PP_06060"
     CDS             complement(3658..4866)
                     /locus_tag="AC16PP_06060"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6481228.1"
                     /translation="MKLEDVDPLDPDIVECPYEFYRALRERGVYRVPQRGFFLVGNYD
                     TAMQVIKDTDTFSSKSGVAVPRSPDGSHPSNPREVRTLLTADPPEHRKYRELVNKAFS
                     AKRVASWEPRIREIADELIEGFAGTGTCELVHDFAVPLPLIVICEAMGLPREMLRTFK
                     RWSDGIASLGGMITPEQRRQIAAEQADFAAYVTEMIEERRSNLGDDFVSDLIRARFDG
                     ARPLNIDELTSLVIQFLVAGNETTTNTIASGMWLLLTHPEQLQAAREDPGLIPNLVEE
                     VLRIESPVQAHFRAAARDTELDGVPIPAGAGVGVIFGCCNRDDDKFADAAEFDVRRDN
                     ASRHLAFSQGIHFCPGAPLARLESAIAFERLLERLGNLRLEGDPNDLAHVPSFTHRGL
                     KKLVVRFDPT"
     gene            complement(5030..6190)
                     /locus_tag="AC16PP_06065"
     CDS             complement(5030..6190)
                     /locus_tag="AC16PP_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481229.1"
                     /translation="MKRIPKWLSGGGLAALLLVLGGQAFGKACADLIAGQHTLVGTVC
                     VGVADDSVQVEYTVIAGWSINSVHFFFDDKPTEEELSVNSCPLGPNFPMTDPKDPYDC
                     GNPKLGHFTYNTSGLNTSTYVFSLPLNGIDYLEHGDLCGSEFNIVAHADVTGPEGQTE
                     GAWAVSWGEMEIDGQMVSFPNYILVDPEDPHERPRGNWGFAGMFDMKVNCEGSFKCET
                     AFAKGDYTFETWPHLDFNSNRWGWLLGPFGKGFVANSESFPLYAGAGQNDISKGALAG
                     VVTVSWDTAYEANQVDFKHHNVAVSYNMNPEWFISSTAENDPTQLLPGSVHIFIGAEG
                     ELLTTAPGQYTYVLDQSEAEVYAYSMSPVTVDAEEFWVVAHAEVCSFVSGEE"
     gene            complement(6433..7740)
                     /locus_tag="AC16PP_06070"
     CDS             complement(6433..7740)
                     /locus_tag="AC16PP_06070"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6481230.1"
                     /translation="MEIRRKQVIERIDPKGQNVGSPADKAPDQDLGHELIPKQRYTSA
                     DFMRREWEGIWSRVWLLGCREEQIAEPGDFICTNIGKESVLLVRQADGGVRAFYNVCM
                     HRGNRLAEEGAGHAETFRCSYHDWQYGLDGRFVDIPDLNTFPQGAPPCAGLREIPCDT
                     WASFVWFSLNEDVEPLKDYMGDLVGHLEPYHFERMAMTRWVTAEWNCNWKASVDAFSE
                     SYHTARTHPQLLWYLDDYNVQIDLYDKHSRYLIPFGLLSPRVDSAPEIPPPLKVMLKN
                     AGIDPASYDGPVGGIRKAVQQHMRESQAALGKDYSELNDDQLTDDYNYLIFPNVTCNT
                     HADNLMLFRNRPHPEDPNRMLFDIWMFELLPEGEERPALPRHDFRPEGSTRSLGLVID
                     QDASNLASVQAGMNSAAYPGLWLSDQELRLRHFHKTISDFVGD"
     gene            complement(7754..>8067)
                     /locus_tag="AC16PP_06075"
     CDS             complement(7754..>8067)
                     /locus_tag="AC16PP_06075"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6481231.1"
                     /translation="AYNALKGSLLVYAKQLSQAVGRDKIRVNCVSPGPIYFEGGVWAM
                     LEDTMAKWFEKTVRDHPQGRLGAPEEVANCILFLSSPAASWVSGTNLIVDGGFTKRVQ
                     F"
BASE COUNT         1440 a         2676 c         2551 g         1400 t
ORIGIN      
        1 agcgctacgg gacccgaacg tgggctgagc tggtcgcacc cgccgtggac ctggctcgga
       61 acggctttac ggtctatccc gccctcgccc aatacgctcg ggaggcactt acggacctcg
      121 ccggcacgaa cttcgctgcc cacttcgccg cgctgacgac caccaggcgt tttcggcagc
      181 cggacctggc cgacaccctc caacgcatcg cagagaacgg ggcagacgag ttctaccgcg
      241 gccgcacggc cagactcttg gccgcggaga tggcacgcgg cggcgggctg atcaccagag
      301 aagacctggc gggctaccgc gccatatggc gcgatcccat cgacttccgc tggcgcgact
      361 accgggtgct cacggctccg cccccgagtt cgggcggcgt ggcgatcgcc cagctgctga
      421 agatgtacga ggcccgcggc ggcgatttcg cggatctcgg gcacaactcc gccgcctatg
      481 tgcatctcaa tgcggaactg gcgaagcgcg tcttcgccga ccgggccgtt tatctggggg
      541 accccgactt cgtctccgtg ccggtcgatt cgctgatcga cgatgactac cttcgcgccc
      601 gggcaagcga agccaacccc gcgcagatca ctcgtccgcg tccggttccc gtcatcgccg
      661 agcacaacga caccacccac ttttccatcg tcgacggggc cggcaacgcc atcgccaaca
      721 cgtacaccat caacacgtct ttcggcagcg gcgtggtcgt cacgggggcc gggtttctga
      781 tgaacaacga gatggacgac ttcgccgtgg cgccgaactc gcccaactac ttcggcctgg
      841 tgggcagcga ggccaacgcc atcgcgcccg gcaagcgcat gctttcctcg atgtcgccca
      901 ccatcgcgct gcgcgacggc aaggtcgcgc tggtcgtcgg cgccatgggc ggctcggcca
      961 tcatcacctc ggtctatcag gtgatgctca acctggaagc cttcgggatg gaggcgccgg
     1021 aagcgttggc agcaacccgc gtgcaccacc agttccctcc cgacgatctg ataacgtacg
     1081 acccgtcggg cgccctgccg accgaagccg tcgcgggcct tgaggcgctc ggctatcggg
     1141 ttgagcccca ctcctgggcg ttcggcaacg ttcagctcat ctggcgcaac cgcgagggcg
     1201 tgctgcaggc ggcatcggac gagcgcttct cgggcgcgag cgaggtgctg cgaactccac
     1261 cgccttgacg ctcacgcagc tcagagcatg atttccaccc gcggcccttc ctcggattcg
     1321 gcctcgtatt ccgggtgaca gcgaaacagg cgcgcggccg gtatgccgcg cacgtcgatg
     1381 aggtagtcgt gcacggccga gccccgggcc ctggcaagct cgagcagggc tgcctcatca
     1441 acgacggccg gggcaccatc cgcgctcgcg ctgccgtccc cctccaccgc ttccgccgct
     1501 gccacatcct ggacagcggc gacgccacag aacgtcaggt gcagacgggg ccgttccagc
     1561 agcaggccgg cgagcttgtc cagatagctc gtgcccagct cgctgagcga ccgcgcgccg
     1621 ggctcgaaag ccaccgggtc gaaacggggc cgtgccgcct ttcccgccat cttgccaacg
     1681 aacatgatgg tgcccagcgg ctgcagcgcg ttcgttgcga cgctgagcgc cgcgcgctgc
     1741 agcgcgttct gcatggccag ccgcacgatg tcgccagtgc cgaatttcgg atcgtggatg
     1801 ctgccggaga tgggcagatc gaacttgatc cgatcgtccc cgtcccgaag cagatccaga
     1861 gcaacgtcca gcggcatgcc gagaccctct tccacgagcc ccggatcgcc cgaccgtttg
     1921 acgcccacct tcagcttctc cagaagcacg gcgttctcgg ctgcgacctc gtcgccgttg
     1981 attcccagct gggtctggat cgaaaggcgg ccccggtcca cccgatagtt tggcaggtag
     2041 ggggtcaact ggctgagatc gaaatggacg acttccaggt ctgccttcac ctgccgggcg
     2101 tcagcgaaca acgccagatc cccggtagcg gagagctgcg cgccattctg ggtgagcgcg
     2161 acgtccagga ccatgggccc ggcagccggg ccgataccca gatcggcaag ccgaaatctg
     2221 tcgatgacga gctccatggt cacctgcggc tcgacggata agtcctccaa acccagggcg
     2281 ctgtttccgc cgatgctcac tgcccccaca tgaagctcga acgacggcgc gccgtcatcg
     2341 gcacccggcg ccgcttccgg ctgagcagac ggctcctcgg cggcagccgg cccaagcagc
     2401 ctgagccgac cttgctctcc acgccgtaac cgggccacga ggtcgtccag ggagacctcc
     2461 ccgattctca acgcgcgagc gctggtatcg agttcgatgc cctccacgga ggccgagccc
     2521 accgacagcc cgacgacctc ttcctcttcc aaaagcctga ggccgcggac cgagagggtg
     2581 tcagacgacg cgcgctcgag cccgtcgacg cgcaccgacg gcgcctccat ggtctccaaa
     2641 cccagcaacc gggcaggcca gtccctatgg tctacgagga ggtcgctcgc ggccacgccg
     2701 atctgcccgg agatcgcagg gggcgagtca cccgattgaa tttcgatctc cccggccaga
     2761 tcgacgtcga gactcagctt acccagatcg atctgcaggg gatgcaaacc gaaggctatg
     2821 tcatcaaccc ccaccgtccc ggccacgttg ccgaacagcc gcccggaagc ctctctgcgc
     2881 agctgcccgc ccaaagaaag cgatagcgat ccgcccggcg actcgatacc tagatcggcc
     2941 accagcggag caaacgcggc aacatcgaaa gcatccacgt cgatcgatat ggtggcgcgc
     3001 cgctcgtctg cgaacgcgaa cacctccgcc tccagcccaa ccggcgcccc gttcagctcg
     3061 agctcggcaa ccagccgagc ggccctctcg ggttcccagg tgaccatgcg atcgatggtc
     3121 agagaacgga tgtagagccg atcgctgagc atcccgtcgt cgtaggtaag ggtcacgttc
     3181 tccaccagga gtccggctaa gccgagcctg ggacgcggtg atccggcggg accctcgccg
     3241 tcgctctcca cttcctgttc cgcctgggcc agggggaaca tcaggccacc cacggagaag
     3301 cgcccgtccg gcatccgccg cacgtccagg acggcatccc gcaggatggc ccgcgaaacc
     3361 atcacccggc cggtaatgag accgagcagc tcgaggtcca gctccagcat ctcgaagctg
     3421 cccgactcat cggcggcgcc gggagcatcc agatactcga cccgtaccct gccggtgaag
     3481 gggttcaagt ccacgtcggt gatacgggcc tcggcaacgc cgcgatcacg cagccagcct
     3541 tccacgtagc gcttgatggc caggggcgcg gccagcccga tgctgatcag cagcagggca
     3601 ataacggccg gaatccagat ctttcggctc acgctgacgg gcaacgtctg catctcatca
     3661 tgtcggatcg aagcgaacta ccagcttctt caggccacga tgggtgaagc tggggacgtg
     3721 ggcaagatca ttcggatccc cttcgagacg caggttgccg agccgttcga ggaggcgctc
     3781 gaaggcaatc gcgctttcca ggcgggccag cggcgcgccc gggcagaagt gaatgccctg
     3841 gctgaaggcg aggtgacgcg aggcgttatc gcgccggacg tcgaattccg ccgcgtcggc
     3901 aaacttgtca tcgtcccgat tgcagcagcc gaagatgacg cccaccccgg ctcccgcggg
     3961 gatcggcacg ccgtcgagct ccgtgtcccg ggcggcagct ctgaagtgcg cctgcacggg
     4021 cgactcgatt ctcagcactt cctcgacgag gttggggatc aggccggggt cctcgcgcgc
     4081 ggcctggagc tgctcaggat gagtgagcag gagccacatc ccggacgcga tggtgttggt
     4141 ggtggtctcg ttacctgcca ccagaaactg gatgacgagg ctcgtaagct catcaatgtt
     4201 cagcggccgt gcgccgtcga agcgggctcg gatcagatcg gagacgaagt cgtcgccgag
     4261 attcgatctg cgctcctcga tcatctcggt gacgtatgcg gcgaaatccg cctgctccgc
     4321 ggcgatctgc cggcgttgct ccggcgtgat catgccaccg aggctcgcga tcccgtccga
     4381 ccagcgcttg aaggtgcgaa gcatttcccg gggcaggccc atggcctcgc agatgacgat
     4441 gagcggcagg ggcacggcaa agtcgtggac gagctcgcag gtgccggtgc cggcaaagcc
     4501 ttcgatcagc tcgtcggcga tctcccggat gcgcggttcc caggaggcca cccgcttggc
     4561 ggagaaggcc ttgttgacca gctcgcggta cttgcggtgc tcgggggggt ccgccgtgag
     4621 cagcgtgcgc acctcgcgcg ggttcgacgg gtggctgccg tccggggatc tcggcaccgc
     4681 cacgccggac ttcgacgaaa aggtgtccgt atccttgatc acctgcatgg cggtgtcgta
     4741 gttgccgaca aggaagaatc cgcgctgcgg cacccggtag acgccgcgct cccgcagcgc
     4801 ccggtaaaac tcgtacgggc actcgacgat gtcgggatcc agcggatcga cgtcttcaag
     4861 cttcatctgc ggttcactcc caccccccga tgcgattctg cgcggagcgc tgccggcgcg
     4921 caatcggccc ggacgtcagc ctcgccccac aagtaaccga gcggccaaaa aaagagggtg
     4981 ccgaagcacc ctccaatccg cctgttcttc tgaacagtgt cctgtcgatc tactcctcgc
     5041 cggagacgaa cgagcaaacc tcagcgtgcg ccaccaccca gaattcctcg gcatccaccg
     5101 tcaccgggct catgctgtaa gcataaacct cggcctcgct ctgatcgagg acgtaggtgt
     5161 actggccagg ggccgtcgtg aggagttccc cctcagcacc gatgaatatg tgcacggatc
     5221 cggggagcag ctgggtcggg tcgttctccg cggtgctcga gatgaaccat tccggattca
     5281 tgttgtagct gaccgcgacg ttgtgatgct tgaagtccac ctggttggcc tcgtaggcgg
     5341 tatcccagct cacggtgacc acaccagcca gagcgccttt ggatatgtcg ttctgaccgg
     5401 ctcccgcgta gagcgggaag gactccgagt tggcgacgaa ccctttgccg aacggcccga
     5461 gcagccagcc ccaacgattg ctgttgaagt caaggtgggg ccaggtctca aaagtgtaat
     5521 cccctttggc gaaggctgtc tcgcacttaa agctaccttc gcagttgacc ttcatgtcga
     5581 acataccggc aaagccccag ttcccgcgcg ggcgctcatg ggggtcttca ggatccacga
     5641 ggatgtagtt ggggaagctc accatctgcc catcgatctc catctctccc cagctgaccg
     5701 cccaagcgcc ttcggtttgg ccttcggggc ccgtgacatc ggcgtgggca acgatgttga
     5761 actcggaccc acagagatca ccatgctcga gatagtcgat gccgttcaac ggcaggctga
     5821 agacgtaggt cgaggtgttc aggccactcg tgttgtaggt gaagtgcccg agcttcggat
     5881 tgccgcagtc gtaaggatcc ttgggatcgg tcatgggaaa attcggaccg agcggacacg
     5941 aattcaccga aagctcttct tccgtcggct tgtcgtcgaa gaagaagtga acagagttga
     6001 tcgaccagcc ggcgatcacc gtgtactcga cctgcacgct gtcatcggca acgccgacgc
     6061 aaacggtacc gacgagggta tgctggcctg caatcaggtc ggcacaggcc ttgccgaagg
     6121 cctgaccgcc taacacgaga agcaaagcgg cgaggccgcc tccgctgagc cacttcggga
     6181 ttctcttcac gagatgcacc tcctagttgt agagaacgcg catccccctc ggcgcgaccg
     6241 gctcctgttt ctatgtcgga ttaccgatct gaagattctg acgattggtg ctggcgttgt
     6301 ggtaaaggag tgtgtccgct ttgtccgggt gggccccggt tacgtagggc aaatgcaaaa
     6361 gtgtcgacca gttcacagaa ctggtcggac gaccgtatcc aagctgaaag gcccgccgtc
     6421 gcgcggtgct cgtcagtcgc cgacgaagtc gctgatggtc ttgtgaaagt ggcgcagccg
     6481 gagctcctgg tcggagagcc agagcccggg atacgcggcg ctgttcatgc cggcctggac
     6541 ggacgcgagg ttcgaggcgt cctgatcgat gacgagcccg agagaccgcg tgctgccttc
     6601 cggccggaag tcgtgcctgg gcagcgcagg ccgctcttcg ccctcgggca gcagctcgaa
     6661 catccagatg tcgaatagca tccggttcgg atcctccggg tggggccggt tgcggaacag
     6721 catcagattg tcggcgtggg tattgcaggt gacgttgggg aagatcaggt agttgtaatc
     6781 gtcggtcagc tgatcgtcgt tcagctccga gtagtccttt ccaagggccg cctggctctc
     6841 ccgcatgtgc tgctggacgg ccttgcggat cccgcccacg ggaccgtcgt aggaagcggg
     6901 atcgatcccg gcgttcttga gcattacctt cagcgggggc ggtatctccg gcgcggagtc
     6961 cacccgcggg ctcagcaggc cgaacgggat caggtaccgg ctgtgcttgt cgtagaggtc
     7021 gatctgcacg ttgtaatcgt ccagatacca aagcagctgg gggtgggtgc gcgccgtgtg
     7081 gtagctctcg gagaaggcgt cgacggacgc cttccagttg cagttccact ccgcggtcac
     7141 ccagcgggtc atggccatgc gctcgaagtg gtagggctcg aggtgcccca ccaggtcgcc
     7201 catgtagtcc ttcagcggct cgacgtcctc gttcaggctg aaccagacga agctcgccca
     7261 ggtgtcgcag gggatctcgc gaagcccggc acagggcggg gcgccctggg ggaacgtgtt
     7321 cagatccgga atgtccacga agcggccgtc caggccgtac tgccagtcgt gatagctgca
     7381 gcggaacgtc tcggcatggc cggctccctc ctcggccagc cgattaccgc gatgcatgca
     7441 gacgttgtag aaggcccgga cgccgccatc ggcctggcgg accagaagga ccgattcctt
     7501 gccgatgttc gtgcagatga agtcgccggg ctcggcgatc tgctcctccc ggcagcccag
     7561 cagccagacc ctgctccaga tgccctccca ctcccgccgc atgaaatcgg cggacgtgta
     7621 gcgctgcttg ggaatcagct cgtggccgag gtcctgatcc ggggctttat ctgcgggcga
     7681 gccgacgttc tgtcctttcg ggtcgatgcg ttcgatgacc tgtttgcggc gaatctccat
     7741 cagcagggac gctctagaac tgcacccgtt tggtgaagcc gccgtcgacg atcaggttcg
     7801 tgccggacac ccagctcgcc gccgggctgg acagaaacag gatgcagttg gctacctcct
     7861 cgggcgcacc gagacgaccc tgggggtgat cgcgcacggt cttctcgaac cacttggcca
     7921 tggtgtcctc tagcatggcc cagaccccgc cctcgaaata gatcgggccg ggggagacgc
     7981 agttgacgcg tatcttgtcg cggcccactg cctgtgagag ctgcttggcg tagacgagca
     8041 gggagccttt cagcgcgttg taggcct
//
LOCUS       DBNHKV010000192        10279 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_98945, whole genome shotgun sequence.
ACCESSION   DBNHKV010000192 DBNHKV010000000
VERSION     DBNHKV010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10279
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_98945"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="AC16PP_06080"
     CDS             <1..374
                     /locus_tag="AC16PP_06080"
                     /inference="COORDINATES: protein motif:HMM:NF047238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR6481232.1"
                     /translation="FAEAGVSPAEMDIAMIYDSFSITVLTILEDLGFCAKGEGGPWAA
                     GGRLRFDRPADGPALNTDGGGLSSNHPGMRGIFLLVEAARQLRGDSCSQVDGAKLAVA
                     HGNGGMLGSRHCAGTVILGRD"
     gene            386..790
                     /locus_tag="AC16PP_06085"
     CDS             386..790
                     /locus_tag="AC16PP_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6481233.1"
                     /translation="MSEAKRPLPNLHEMDSRAFWEATREHELRYQECADCDTVVFYPR
                     RHCTGCLGDALAWKTAKGEGTVYTFSVIRQSYHPFFRALVPYAVAWIDLDEGPRLLSN
                     VIGPVEDIRVGQRVRVAWEDHEELAVPLFEPI"
     gene            complement(844..1257)
                     /locus_tag="AC16PP_06090"
     CDS             complement(844..1257)
                     /locus_tag="AC16PP_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6481234.1"
                     /translation="MTVNVIGDVEFGEELPVFEPDTSLANVKRFTEAAGWQGPRFTDH
                     EAARREGLPGALVPGIMSQGFLAAMIHRWAPESQIESVDTVFRAPVLVDERHSISGVV
                     TDIDEDAGTVEIDLTVTNEANETRVFGTATVRLPA"
     gene            complement(1254..1697)
                     /locus_tag="AC16PP_06095"
     CDS             complement(1254..1697)
                     /locus_tag="AC16PP_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAS1-like dehydratase domain-containing protein"
                     /protein_id="HIR6481235.1"
                     /translation="MLDLEALRANFLNEDFDEQHFEVNAEQCVAYALACGETAPRYTD
                     PAHPDFQAPPTLPSSFHPGRRLPDGFPKLPGLGMDAGKAVAPKRPIRPGVPLVGRTHL
                     HDVYAKTGRSGRMTFMVTRMEIYDPDGEQLATADTRIVIRERPDQ"
     gene            complement(1707..2336)
                     /locus_tag="AC16PP_06100"
     CDS             complement(1707..2336)
                     /locus_tag="AC16PP_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HIR6481236.1"
                     /translation="MILDLHTHSIKSDDGRAKVQNYCQWIRTREIPIDGLVLTEHRQF
                     DAESDYSPLAQQFGITILKGSEVETEYGHVLVFGVTEALTRAFDFADIHLPLARVIEA
                     CALHGAVPVPCHPGRRRVGMSAHLEEFGTPEGVRIVETHNGGSRGDEDAVAQAMAAEL
                     GYLGIGGSDAHIVSHVGRCATRFPGTISNEQELVEALLAEDFEAVTNRE"
     gene            complement(2417..4357)
                     /locus_tag="AC16PP_06105"
     CDS             complement(2417..4357)
                     /locus_tag="AC16PP_06105"
                     /inference="COORDINATES: protein motif:HMM:NF025114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6481237.1"
                     /translation="MGQLKFDHLQPIVDDDATIEAALADAHIPSLMAAMVHVSGDPSW
                     IRGAIRPVNQFFGDSQGGIAPNDQAEVRRRALEALIAYRDGKATPPPPDPATVSEMVN
                     FVIGQEVTSDYGEFLHAELALDGADPYASEGFDEISEAARREFKVIIVGAGMSGLLAA
                     IRLKEAGISWEIVEKHADVGGTWYQNGYPGCRVDSPNHIYSYSFRPADWPQYYSSQEV
                     LREYFDKVATEYGLRDQIRFSTEVQSMAFDDASGTWQVEVTNGTGARETLTANAVISA
                     VGQLNRPKWPDLAGQDRFRGVSFHSTEWEHEHDLSGKRIIVIGTGASAFQFAPEIAKV
                     AAEVEIFQRTPNWMAPVATYHDHIPLGKHWLLNHVPYYAKWYRFAMFWNMAEGVLSAV
                     RKDPAWNQTEDAISAENDTLRQMFTDWLEELCEGDEELLEKVVPKFPVGGKRVLLDNG
                     NWIRALRRDNVHLLTDPIAEINETGVVTEDGKQHDADVLIYGTGFQASRFLWPMKVTG
                     RDGIDLNEQWQGDARAYLGITIPNFPNLFCCYGPNTNIVVNGSIVFFSECEVRYILSC
                     IRQLLAGGHKALDCRADVHDAYNEWIDEGNANMAWGAARVPSWYKNEKGRVSQNWPFT
                     LLEYWNHTRDVNAADYEFLSDA"
     gene            4511..5596
                     /locus_tag="AC16PP_06110"
     CDS             4511..5596
                     /locus_tag="AC16PP_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6481238.1"
                     /translation="MDIGICVASHIGDLDYVVRAEALGYSHAWLADSQMLWSDCYATL
                     ALAADRTQKIRLGTGVAVSGTRPAPVNAAAIATINAIAPGRTFLGVGAGNTAMRVMGQ
                     PPHRIAEFDRYLSTLVPLVRGEEAQMGPLTVGARGEAPIRHIMPDKGFVNFDDRIPVY
                     VSGFGPRSLGLAGKHGDGAVLAMPANPAVMANIWRMLEEGAAAAGRTLDRSDFYTTGL
                     TAIVVLDPGEASDSPRVRAECGAMAMASVHYAYDQWRNFGHQPPGYLGGIWDDYCALL
                     ESYPQERRHQRIHAGHNCWVLPEEERFLTREVLETTCMIGTRDQLIDRLAALSEAGLD
                     QVMILPNFDTRFDVLERVGRELVGRIP"
     gene            complement(5658..6764)
                     /locus_tag="AC16PP_06115"
     CDS             complement(5658..6764)
                     /locus_tag="AC16PP_06115"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR6481239.1"
                     /translation="MIATFDNVDELGPEKVIEIYDPSTGLKAIVVVDNVAAGPAIGGV
                     RMAVDATREECGRLARAMTLKNAMAGLAHGGAKSVIVADPAMADEAKEVLIRSFAHAI
                     ADLRDYIPGPDMGTDETSMALVRDVTGRSAGLPRELGGIPLDEIGATGLGLSVSLEVA
                     EAFTDLSLDGARIVVQGFGSVGQHAARFLARRGARLVGVSDSRGARAAAEGFDLDLLL
                     ASKQEGVSVGAFDEGEAIDPEDLVELDCDVWIPAARPDVLNGANAGRIRAKVVAQGAN
                     IPATPEAEQTLAARGVLVIPDFVANAGGVICAAVEYHGGSESQALATIQDKIHGNVKE
                     VLSRATTTGELPRKVAMDIARERVQAAMRYRKTY"
     gene            complement(6761..7741)
                     /locus_tag="AC16PP_06120"
     CDS             complement(6761..7741)
                     /locus_tag="AC16PP_06120"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR6481240.1"
                     /translation="MAEHTLLEAVNLALRRAMADDDRVLVLGEDVGVVGGVFRATDGL
                     LQAFGPHRVFDTPLAETGIAGVAVGLAVQGFRPVAEIQFMGFIYAAIDQLANHASRMR
                     TRTRGRQSCPLVLRAPFGGGIHAPEHHSESAEAMLAHIPGLRVVIPSSPRTAYGLLLA
                     AIRDPDPVVFLEPKRLYRSAKEAFEDDGAALPLDRCHIVRPGKDLTLVTWGAMVQETL
                     AAAEQLSGEGVAAEVIDVATIKPLDGETICESVGRTGRLVIVHEAPITGGFGGEIAAC
                     VAERALYSLQAPILRVAGYDTVMPLARLEHHYMPSVSRLVGAARETLEAS"
     gene            complement(7734..8810)
                     /gene="pdhA"
                     /locus_tag="AC16PP_06125"
     CDS             complement(7734..8810)
                     /gene="pdhA"
                     /locus_tag="AC16PP_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HIR6481241.1"
                     /translation="MSDSLPFGVHPSTIIGPDGNLLRPIPEPLVDPATIRALYGWMHR
                     TRAFDAKAIELQRTGRLGTYASCLGQEAASVGIGHAMAPEDVLVPSFRDQGTQLYRGV
                     SMVELLLYWGGDERGSDYAVPRDDLPISITVGGHAPHAAGVALALKLRAEPRVAVCVF
                     GDGATSKGDVYEAMNIAGVWRLPVVFVVNNNQWAISTPRADQSAATTLAQKAVAAGFE
                     GSQVDGNDVLAVSEAVGDAIGHARKGDGPRLVECLTYRLGDHTTVDDASRYRSEAEVA
                     RWRQLDPLARVGAYLAQHEGWSDADEDALVERCDDEVASAATAYNGTTPQPPEAIFDH
                     LFASLPRELERQRATLLASAPADG"
     gene            complement(8836..>10279)
                     /locus_tag="AC16PP_06130"
     CDS             complement(8836..>10279)
                     /locus_tag="AC16PP_06130"
                     /inference="COORDINATES: protein motif:HMM:NF023282.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR6481242.1"
                     /translation="FHHFDFERNRDALALDLDLSSRAKLNLGFARYEKRGESTTVLDV
                     EREEFELDKPIDESLDRYELGFTYAWDHVTVALGQTWQEYDNDSQIFLPGFSPGSEAD
                     APTSLDLFFLDQPYGFTSSDQRLSLQVRPSSQWIVELDARAGNLDLDMTATERAQGTD
                     YLGRPFDREDAGAADVDRDTLLLDASVAYLVNDRLQLNAGVRRYDLEQDGAVLFGDTA
                     GASAWDIETTGFELGVDMAVTTTVTLSGGWSTERRKSVVSHAIEGAGHTEAVDTDRDG
                     FFGRLSYRPSRRAQVTLSFEDDRIDDPYTLASATDARRYKARASYRWDNGLKVSASYR
                     RTDYENDNTGWISESRQSDIRLTHAGDRLTVSVGAAFVELQREVDQLVTGGSRQDLFA
                     ISYGADADFWDGAVRWHVTDRVDLTASYRAYENEGSFRVDRDDVRAGVEFLLPADYAL
                     LLSYRNVDYNEDELESFDADVWELALKFRW"
BASE COUNT         1617 a         3357 c         3460 g         1845 t
ORIGIN      
        1 cctttgctga ggcgggcgtc tctccggccg agatggacat cgccatgatc tacgattcgt
       61 tcagcatcac ggtgctcacg atcctggagg atctgggatt ctgcgcgaag ggggagggcg
      121 ggccctgggc cgcgggaggg cgtttgcgct tcgatcgccc cgcggatggc ccggccctga
      181 acaccgatgg cggcgggctc tcgtcgaacc atccgggcat gcggggcata tttcttctgg
      241 tcgaagccgc gcgccagctg cgcggtgatt cctgctctca ggtggacggt gcgaagctgg
      301 ccgtggccca cggcaacggc ggcatgctcg gcagccggca ttgcgcgggt accgtgatac
      361 ttgggcggga ttgaggagta gcaccatgag cgaagcgaag agaccgctgc cgaacctgca
      421 cgaaatggac agccgggcat tctgggaagc cacccgggaa cacgagctgc gctatcagga
      481 gtgcgcggac tgcgacaccg tcgtcttcta cccgcggcgg cattgcacgg gttgtcttgg
      541 cgacgcgctg gcctggaaaa cggccaaggg ggagggcacc gtctacacgt tcagcgtgat
      601 caggcagagc tatcacccct tcttccgcgc tctggtcccc tacgcggtgg cctggatcga
      661 cctggacgag gggccgcggc tactgtccaa cgtgatcggc cccgtggagg acattcgggt
      721 gggccaacgg gtgcgcgtcg cgtgggaaga ccacgaagag ctggctgtgc cccttttcga
      781 gccgatctga acgaccggtt gctgtcgcct ttgccggttg tgatcggcgg aaccggcggg
      841 agatcaggcg gggaggcgaa cggtggcggt gccgaagacc cgggtctcgt tggcctcgtt
      901 cgtcacggtc agatcaattt cgaccgtgcc cgcatcttcg tcgatgtcgg tgacgacgcc
      961 gctgatgctg tgcctttcgt caaccagcac gggcgccctg aacacggtgt ccacgctttc
     1021 gatctgactc tccggcgccc agcgatggat catggccgcg agaaaaccct gactcatgat
     1081 gcctggcacc agcgctccgg ggagcccctc gcgcctggcg gcctcatggt ccgtgaaccg
     1141 gggtccctgc cagccggcgg cttcggtgaa tcgcttgacg ttggccaggc tcgtgtccgg
     1201 ctcgaagacc ggtagctctt cgccgaactc gacgtcgccg atgacgttga cggtcattga
     1261 tccggcctct ctcgaatgac gattcgggtg tcagcggttg ccagctgctc gccgtcggga
     1321 tcgtagatct ccatgcgcgt aaccatgaac gtcatgcggc ccgatcgtcc ggttttggcg
     1381 taaacgtcgt ggagatgcgt gcgcccgacc agcggcaccc cgggccgtat gggccgcttc
     1441 ggcgcaacag ccttgcctgc gtccatgccc agaccgggta gtttgggaaa gccgtcaggc
     1501 aggcggcggc cggggtgaaa gctcgacggc agcgtgggcg gcgcctggaa gtcaggatgg
     1561 gccgggtcgg tgtagcgcgg cgccgtctcg ccgcaggcca gggcgtaggc aacgcactgc
     1621 tcggcgttca cctcgaagtg ctgctcgtcg aagtcttcgt tgaggaagtt tgcccgtaac
     1681 gcctctaggt caagcatggg tgtctttcac tcccgattgg taacggcttc gaagtcttcg
     1741 gccagcagcg cttccaccag ttcctgctcg ttggagatcg tgcctgggaa acgggtagcg
     1801 cagcgtccca cgtggctgac gatgtgcgcg tcgctgccgc caatgcccag gtagccgagc
     1861 tcggcggcca tggcctgggc cacggcgtcc tcgtctccac ggctgccgcc attgtgcgtc
     1921 tccacgatcc tcaccccttc cggcgtgccg aattcctcga gatgagcgct catgcccacg
     1981 cgccggcgcc cgggatgaca gggaacggga accgcgccgt gcagcgcaca ggcttcgatg
     2041 acccgggcca aaggaaggtg gatgtccgcg aaatcgaacg cccgggtcag cgcttcggtc
     2101 acgccgaaca ccaggacgtg gccgtactcg gtctcgacct cgctgccctt gaggattgtg
     2161 atcccgaact gctgagccag cggggagtag tcggactcgg cgtcgaactg gcgatgctcg
     2221 gtgagcacga ggccgtcgat ggggatctcg cgtgtgcgga tccactggca gtagttctgc
     2281 accttggcac gaccatcatc ggacttgatg gaatgtgtgt gcagatcgag aatcaccgaa
     2341 cctccttgaa cgacccgaag cgccgcgagc gcgcaatctt aacgtcgcgc gccggcttct
     2401 gcgagcgcgg ggcgcgtcac gcgtcggaga ggaactcgta gtccgccgcg ttgacgtccc
     2461 tggtgtggtt ccagtactcg agcagcgtga agggccagtt ctgggacacc cggcctttct
     2521 cgttcttgta ccaggagggt acccgggccg cgccccaggc catgttggcg ttgccctcgt
     2581 cgatccactc gttgtacgca tcgtgaacgt ctgcgcggca atccagggcc ttgtggccgc
     2641 cggccagcag ctggcggata cagctcagga tgtagcgcac ctcgcattcg gagaagaaga
     2701 cgatgctgcc gttgacgacg atgttggtgt tgggcccgta gcagcagaaa agattgggga
     2761 agttggggat ggtgatgccg aggtaggcgc gggcgtctcc ctgccactgc tcgttcaggt
     2821 cgatcccgtc gcgaccggtg accttcatcg gccatagaaa ccggcttgcc tggaagccgg
     2881 tgccgtagat cagcacgtcg gcgtcgtgct gtttgccatc ctcggtgacg acgccggtct
     2941 cgttgatctc ggcgatggga tcggtgagca ggtgcacgtt gtcgcgcctc aaggcgcgaa
     3001 tccagttgcc gttgtcgagc agcacccgct tgccgcccac cgggaacttg ggcaccacct
     3061 tttcgagcag ttcctcgtcg ccttcacaca gctcttcgag ccagtcggtg aacatctgcc
     3121 ggagcgtgtc gttttcggcg ctgatggcgt cttcagtctg gttccaggcc ggatccttgc
     3181 gcaccgcgga gagcacgccc tcggccatgt tccagaacat ggcgaagcgg taccacttcg
     3241 cgtagtaggg aacgtggttg aggagccagt gcttccccag cggtatgtgg tcgtggtagg
     3301 tggccaccgg ggccatccag ttgggtgtgc gctgaaaaat ctcgacctcg gctgccacct
     3361 tggcgatttc gggagcgaac tgaaacgcgc tggcaccggt gcctatgacg atgatgcgct
     3421 tgccggacag atcgtgctcg tgttcccact cggtggagtg aaaggacacg ccgcgaaaac
     3481 gatcctggcc ggcgaggtcg ggccatttcg gtcggttcag ctggcccacg gcgctgatga
     3541 cggcgttcgc ggtgagggtc tccctggcgc cggtaccgtt cgtgacttcc acctgccacg
     3601 tcccggacgc atcgtcgaac gccatggact gcacttccgt gctgaaacgg atctggtcgc
     3661 gcaggccgta ctccgtcgcc accttgtcga aatactcccg cagaacttcc tggctcgagt
     3721 agtactgggg ccagtcggcg ggccggaagc tgtaggagta gatgtgattg gggctgtcga
     3781 cccgacaccc cggatagccg ttctggtacc aggtcccgcc cacgtcggcg tgcttctcca
     3841 cgatctccca ggagatgcca gcctccttga gcctgatcgc cgcgagcagc cccgacatcc
     3901 ctgcgccgac gatgatgacc ttgaactccc gccgtgccgc ctcggagatc tcgtcgaagc
     3961 cctccgaagc gtaggggtcg gcgccgtcca gggcgagctc ggcgtgcaga aactccccgt
     4021 agtccgaggt gacttcctgg ccgatgacga agttcaccat ctcgctgacg gttgcggggt
     4081 cgggaggcgg gggcgtcgct ttgccatcgc gataggcaat cagcgcctcg agcgcccgtc
     4141 tgcggacctc tgcctgatca ttgggcgcga tgcctccctg ggagtcgccg aagaactggt
     4201 tgaccgggcg aatggctccg cggatccagc tgggatcacc gctgacgtgc accatggcgg
     4261 ccatcagcga cgggatgtgg gcatcggcga gggcggcctc gatggtcgcg tcgtcgtcga
     4321 cgatgggctg caggtggtcg aacttgagct gccccatggc tgatttcccg gctggatatg
     4381 ccgatccatt tagcgcaggt agccgttcgg atgcaagggg acggctgggg cgtcccggcc
     4441 aggatcgtct ggcaagcgct ggcgccctta agtacgctaa tcggccaggt tcactatccc
     4501 ggagatcagg atggacatcg gaatctgcgt cgcctctcac atcggcgatc tcgactacgt
     4561 cgttcgcgcc gaggccctcg gatattccca cgcctggctc gccgacagcc agatgctctg
     4621 gtccgactgt tacgccactc tggcgctggc cgcggaccgg acccagaaga tccgcctcgg
     4681 caccggcgtg gccgtctcgg gcacccggcc ggcacccgtc aacgcggcgg ccattgcgac
     4741 catcaacgcc atcgcgcccg ggcggacgtt cctcggggtc ggcgccggca acacggcaat
     4801 gcgcgtcatg gggcagccgc ctcatcgaat tgccgagttc gaccggtatc tgagcaccct
     4861 cgtgccgctg gtccgtggcg aggaggcgca gatgggacct ttgaccgtcg gcgcccgcgg
     4921 cgaggcgccc attcggcaca tcatgccgga caaggggttc gtcaatttcg acgatcgaat
     4981 ccccgtctac gtttccggct tcggaccgcg gtctctgggt ctggcgggca agcacggcga
     5041 cggcgcggtg ctggccatgc cggcgaaccc ggcggtgatg gccaacatct ggcgcatgct
     5101 ggaagagggc gcggcggcag cgggccggac gctggatcga tcagacttct acacgaccgg
     5161 actgaccgcc atcgtcgtgc tggacccggg ggaagcgagc gattcgcccc gggtgcgagc
     5221 cgaatgcggc gccatggcca tggcctccgt tcactacgcc tacgatcagt ggcgcaattt
     5281 cggtcaccag ccgcccggct atcttggcgg catatgggac gactactgcg ctctgctcga
     5341 atcttatccg caggaaaggc gccaccagcg gatccatgcc ggccacaact gctgggtgtt
     5401 gccggaagag gagcggtttc tgacccgcga ggtgctcgag accacctgca tgataggcac
     5461 tcgcgaccag ctcatcgacc ggcttgccgc cctcagcgaa gcgggtctgg atcaggtgat
     5521 gatcctgccc aatttcgata cccggttcga cgtgctcgag cgggtcggcc gggagctggt
     5581 gggccgtatt ccctgagcca ggggcgcgct gggtcttcgc cggggcgagg cgctgccgtc
     5641 cggggcatca ctccgggtca gtaggtttta cgatagcgca tggccgcttg cacccgttcg
     5701 cgagcaatgt ccatggcgac ttttctcggc agctcgcccg tggtggtcgc cctggagagc
     5761 acctctttga cgttgccgtg aatcttgtcc tggatcgtgg ccagggcctg gctttcactg
     5821 ccgccgtgat actccacagc cgcgcagatg accccgccgg cgttggcgac gaagtccggg
     5881 atcaccagaa cgccgcgcgc cgccagggtc tgctccgcct ccggggtggc ggggatgttc
     5941 gctccctggg caactacctt ggctcggatc cggccggcgt ttgccccgtt gagcacgtcc
     6001 ggccgggcgg ccggaatcca cacgtcgcaa tcgagctcga cgagatcctc gggatcgatg
     6061 gcctcgcctt cgtcgaaggc gccgacgctg acgccctcct gcttggatgc gagcaggaga
     6121 tcgagatcga aaccctctgc ggccgcccgc gctccccggc tgtcggagac gcccaccagc
     6181 cgggcgccgc gtcgggcgag aaagcgggcc gcgtgctggc ccacggagcc gaatccctgg
     6241 accacgatcc tggcgccatc gaggctgaga tccgtaaagg cctcggcgac ctccagggaa
     6301 acgctcaagc caagacccgt cgcgccgatc tcgtccaagg gaatgccccc cagctcccgc
     6361 ggcaggccgg cggaccggcc ggtgacatcc ctgaccagtg ccatggaggt ctcgtccgta
     6421 cccatgtcgg gaccgggtat gtagtctcgc aggtcggcga tggcgtgggc gaagcttcga
     6481 atcaggactt cctttgcttc gtcggccatg gccgggtcgg cgacgatcac agacttggcg
     6541 ccgccgtgag caagccccgc catggcgttc ttcagcgtca ttgccctggc aagccgccca
     6601 cactcctcgc gcgtggcgtc gacggccatc ctgaccccgc cgatggccgg cccggcggcg
     6661 acgttgtcca ccaccacgat ggccttcagg cccgtgctcg ggtcgtaaat ctcgatgacc
     6721 ttctcgggcc cgagctcgtc gacgttgtcg aaagtagcga tcatgaagcc tccagcgttt
     6781 cccgagcggc gccgacgagt ctcgacacgc tcggcatgta atgatgttcg agcctggcca
     6841 gcggcatcac ggtgtcgtag ccggccaccc tcaggatggg tgcctgcagg ctgtacagcg
     6901 cgcgttcggc cacgcaggcg gcaatttctc cgccgaaacc gcccgtgatc ggcgcttcgt
     6961 ggacgatgac gagacggccc gttcgcccca ccgattcgca gatcgtctcg ccgtccaggg
     7021 gtttgatcgt ggcgacgtcg atgacctctg cggcgacgcc ttcgccggac agctgctcgg
     7081 cggcggccag ggtttcctgg accatggctc cccaggtgac gagggtgaga tcctttccgg
     7141 gacggacgat gtggcaacgg tcgagcggga gggctgcgcc gtcgtcttcg aaagcctctt
     7201 tggccgaccg gtacaggcgc ttcggctcca ggaagaccac cgggtccggg tcccggatgg
     7261 ctgccagaag cagcccgtag gcggtccgcg gcgacgaggg aatgaccacc cgcagccccg
     7321 gaatgtgagc cagcatggcc tcggcgctct cggagtgatg ttccggcgcg tggatgccgc
     7381 caccgaacgg cgcgcgcagc accagcgggc agctctggcg cccgcgggtg cgggtccgca
     7441 ttctcgaagc gtggttcgcg agctgatcga tcgcggcgta gatgaacccc atgaactgga
     7501 tctccgccac gggccggaac ccctgcacgg cgaggccgac ggccacgccg gcgatgccgg
     7561 tctcggccag gggggtatcg aagacccggt gcggcccgaa ggcctgcagc aggccgtccg
     7621 ttgccctgaa cacgccgccc accaccccaa cgtcctcgcc gagaacgaga acacggtcgt
     7681 catcggccat ggcgcggcgc aacgccaggt tcaccgcctc cagcagggtg tgctcagcca
     7741 tcggccgggg cacttgctag cagggtcgct ctctgtcgtt ccaactcccg gggaaggctg
     7801 gcgaagagat ggtcgaagat ggcctcgggt ggctggggcg tcgtcccgtt gtaagcggtg
     7861 gctgccgacg ccacctcgtc gtcgcaccgc tcgaccaggg catcctcgtc ggcgtcgctc
     7921 catccctcat gctgggcgag ataggcaccc acgcgggcca agggatccag ctgccgccag
     7981 cgcgccacct cggcctcgct tcggtagcgg ctggcgtcgt ccacggtggt gtgatcgccc
     8041 agccggtagg tcaggcattc gaccagtcgt ggcccgtcac ccttgcgggc atggccgatg
     8101 gcgtcgccca ccgcctcgct gacggcaagc acatcgttgc cgtccacctg gctgccctcg
     8161 aaaccggcgg ccaccgcttt ctgcgccagg gtggtggccg ccgactgatc ggctcggggc
     8221 gtggagatgg cccactggtt gttgttcacg acgaagacga cgggcagccg ccagacgccg
     8281 gcgatgttca tcgcctcgta cacatcgccc ttggacgtag cgccgtcgcc gaacacgcac
     8341 acggccaccc ggggttcggc tcggagcttc agcgccagcg cgacgccggc cgcatgggga
     8401 gcgtggccgc ccacggtaat gctgatgggc agatcgtccc tcggcacggc gtagtcggag
     8461 ccgcgctcgt cgccgcccca gtagagcagc agctcgacca tgctcacacc ccggtacagc
     8521 tgggtgccct gatctctgaa cgaaggcacc agcacgtcct cgggcgccat ggcgtgaccg
     8581 atgccgacgc tggccgcctc ctggccgaga caggaggcgt aggttcccag gcgtccggtc
     8641 cgctggagct cgatggcctt ggcgtcgaag gcgcgggtgc ggtgcatcca cccgtagagg
     8701 gctcggatgg tggccgggtc caccaggggc tccgggatcg gccgcaggag attgccgtcg
     8761 ggcccgatga tggtgctggg gtggactccg aatggaaggc tgtcgctcat ggtcggctcc
     8821 ggcacgtcgt gcgcgtcacc agcggaactt cagggccagc tcccacacgt cggcatcgaa
     8881 agattcaagc tcgtcctcat tgtagtcgac gttgcggtag ctgagcagca gggcgtagtc
     8941 cgcgggcagc agaaactcga cgcccgcgcg gacgtcgtcc cggtcgaccc tgaagctgcc
     9001 ctcgttctcg taagctcggt agctggcggt caggtccacc cggtcggtga catgccaacg
     9061 aacggcgccg tcccagaaat ccgcatccgc gccgtaggag atggcgaaca gatcctgccg
     9121 ggagcccccg gtcaccagct ggtccacttc ccgttggagc tcgacgaagg cggctcccac
     9181 cgacaccgtc aggcggtcgc ctgcgtgggt caggcggatg tcggactgac ggctttcgga
     9241 gatccagccc gtattgtcgt tttcgtagtc cgtgcgccgg taggacgcgg aaaccttcag
     9301 gccgttgtcc caccggtagg atgcgcgcgc cttgtagcgt cgggcatcgg tagccgatgc
     9361 cagcgtgtag ggatcgtcga tccggtcgtc ctcgaagctc aaggtgacct gggctcttcg
     9421 gcttggccgg tagctgagcc gcccgaagaa gccgtcccgg tcggtatcca cggcttcggt
     9481 gtggccggcg ccttcgatgg cgtggctgac gacgctcttc cgccgctcgg tggaccatcc
     9541 gccgctgagt gtcaccgtgg tcgtcacggc catgtctacc ccgagctcga acccggtggt
     9601 ctcgatgtcc cacgcgctcg cgccggcggt gtcgccgaac agcaccgcgc cgtcctgctc
     9661 cagatcgtag cggcgcacgc cggcgttgag ctggagacga tcgttgacca ggtaggcgac
     9721 cgaggcgtcg agcagcaggg tgtccctgtc cacgtctgct gcccccgcgt cctcgcggtc
     9781 gaacggacgc ccgaggtagt cggtgccctg ggcccgctcc gttgccgtca tgtccaggtc
     9841 caggttgccg gcccgcgcgt cgagctccac gatccactgg gatgatggcc gcacctgcaa
     9901 gctcagacgc tgatcgctgg acgtgaagcc gtagggctgg tccagaaaaa agagatccag
     9961 cgaggtgggt gcgtccgcct cgctgcccgg cgagaacccg ggcagaaaga tctgcgagtc
    10021 gttgtcgtac tcctgccagg tctggcccag ggccacggtg acgtgatccc aagcgtaagt
    10081 gaagccgagt tcgtatctgt ccagggactc gtcgatgggc ttgtcgagct cgaattcctc
    10141 ccgctccacg tccagcacgg tggtgctctc gccgcgtttc tcgtatcggg cgaatcccag
    10201 gttcagcttg gcccggctgc tcagatccag atccagcgcg agggcgtcgc gatttctctc
    10261 gaagtcgaaa tgatggaag
//
LOCUS       DBNHKV010000193         3271 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_806756, whole genome shotgun sequence.
ACCESSION   DBNHKV010000193 DBNHKV010000000
VERSION     DBNHKV010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_806756"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="AC16PP_06135"
     CDS             complement(<1..1121)
                     /locus_tag="AC16PP_06135"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6481243.1"
                     /translation="MAEKVETVRTTDPAYHRLLDTETRPVPESYRRDSPIEPGPTFVP
                     VERYISREFFDLEVEKIWKRVWQMACHEDDLPDVGDYVPYDIAGMSFLVVRTGEDEFK
                     AYYNACLHRGRKLRENRGKAATDLRCPFHGWTWNLDGSLKQIPCQWDYPGLKMSEQSL
                     PEVKVGRWGRYIFINPDPDCGPLEDFLGDLSSHFELLPYEKRYKEAHVARIIRCNWKV
                     ASEAFMESYHVIATHPQIILGGVHDTDTKYDVFGNYSRAIRCGALESSGLPDWGELPQ
                     DGRRRVRHPLNGFVYEATDDGLVQVTNPEGKTGLFTANADHVSGELGDVNPHLCNWVG
                     GRQLEPSEFEKAMASRAAKQSGETVKHPRVLFADIQREAL"
     gene            1285..2682
                     /locus_tag="AC16PP_06140"
     CDS             1285..2682
                     /locus_tag="AC16PP_06140"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6481244.1"
                     /translation="MDYDLTKPADPTLAPGDARCPGPSTRDIILKEQDGAPAALTAES
                     WSFLGDEDIPFSRYTSQPFYDREIETIWRKTWQWACREEHIPNPGDWTVYDVGPYSVV
                     VIRDEGGAVKAYVNSCPHRGMQFCDAGSSGRGKQFLRCPFHGMSWHLDGSLREIPCRW
                     DFPHIEDENFGLTEVPTGTWAGFVFINLDPDCEPLESYLEVLPEHFKNWKLEDRFVAL
                     HTRKILPGNWKMCMEAFLEAYHVLATHPEGIHSAGDANADYDIFGRHVTRFVHTIGYP
                     SPHVASEVDERKLFAALGHNPDDLPDGMSARQRHADIMREQLGREWDVDLSGLSNSEM
                     LDSIEYFVFPNACFFPGINIRLVYRFRPIDVDHTMHEILLLQPVPEHGERPKPAEPID
                     LTIDESYEKANEVSLGGFALAMVLDQDTENFHRQRAGIKAAQRAGVRTAAVLANYQES
                     RIRRLHMTVDEYMAG"
     gene            2705..>3271
                     /locus_tag="AC16PP_06145"
     CDS             2705..>3271
                     /locus_tag="AC16PP_06145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019750117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR6481245.1"
                     /translation="MIIGAGTAGCVLANRLSADKDADVLLLEAGGKDDYFWIDIPVGY
                     LYTINNPRTDWCYQVEPEPGLNGRRINYARGKVLGGCSSINAMIYMRGQRGDYDHWAQ
                     LGNRGWSWDDVLPVFKRSEDYQHGAGEFHGAGGELRVEERRVDWEILDAWRDAAQECG
                     IPKIEEFNRGDNFGNAYFQVNQRRGVRWS"
BASE COUNT          587 a         1087 c         1036 g          561 t
ORIGIN      
        1 agcgcctcgc gctggatgtc ggcgaagagc acccgcggat gcttgacggt ctcgcccgac
       61 tgcttggccg cccgggaggc catggccttt tcgaactcgg acggctccag ctggcggccg
      121 ccgacccagt tgcacaggtg cgggttgaca tcccccagct cgccggagac gtggtccgcg
      181 ttcgccgtga acagccccgt cttgccttca gggttggtca cctggaccag cccgtcgtcc
      241 gtggcttcat aaacgaagcc gttcaacggg tgacgcaccc ggcgccgacc gtcctggggc
      301 agctcgcccc aatcgggcag gccgctgctc tccagggcgc cgcagcggat ggcccgcgag
      361 tagttgccga acacgtcgta cttggtgtcc gtgtcgtgga ccccgcccag gatgatctgc
      421 gggtgggtgg cgatcacgtg gtaagactcc atgaacgcct cggaagccac cttccagttg
      481 cagcggatga tcctggctac gtgggcctct ttgtagcgct tctcgtaggg cagcagctcg
      541 aagtggctcg agaggtcgcc cagaaagtct tccagcggcc cgcagtccgg atcggggttg
      601 atgaagatgt agcggcccca gcgacccacc ttgacctcgg gcaggctctg ctccgacatt
      661 ttcaggccgg gatagtccca ctggcacggg atctgtttga gcgaaccatc caggttccag
      721 gtccagccgt gaaacgggca acgcagatcg gtggcggcct tgccccgatt ttcacgcagc
      781 ttgcggcccc ggtgcaggca ggcgttgtag taggccttga actcgtcctc cccggtccgg
      841 accaccagga acgacatgcc ggcgatgtcg tagggcacgt agtcgccaac gtcgggcagg
      901 tcgtcttcat ggcaggccat ctgccagacg cgcttccaga tcttctctac ctcgaggtcg
      961 aaaaactccc tggagatata gcgctccacc ggcacgaagg tgggtccggg ctctatggga
     1021 gaatcgcggc ggtaggactc cggaaccgga cgggtctcgg tatccagcag ccggtgatag
     1081 gcggggtccg tggtgcggac ggtttcaact ttttctgcca tgacgcgttc tcccctgaac
     1141 tgccggaata ctaccccgat ctggtcacaa ggaatacggc tcgcgcggag cccgccaggt
     1201 aacccgatga cgtcaccgcg ctccggttgg ctcgctggcc gaaccatgta tcatcagtgc
     1261 agcacctacc cggggaatac caagatggac tacgacctca ccaaaccagc cgacccgacg
     1321 ttggctccgg gagacgcgcg ctgtcccgga ccgagcaccc gcgacatcat cttgaaggag
     1381 caggacgggg cgcccgccgc gctcacggcc gaaagctggt cgttcctcgg agacgaggac
     1441 attccgttct cgcgctacac gtctcagccg ttctacgacc gggaaatcga aaccatctgg
     1501 cgcaagacct ggcagtgggc ttgccgcgag gagcacatcc ccaacccggg tgactggacc
     1561 gtttacgacg tagggccata ctcggtggtc gtcatccgcg acgagggcgg cgcggtcaaa
     1621 gcctacgtca attcctgccc gcaccggggc atgcagttct gcgacgcggg ctcatccggg
     1681 cgcggcaagc agttcctgcg ctgtcccttt cacggcatgt cgtggcacct ggacggctcg
     1741 ctcagggaga ttccctgccg ctgggacttc ccccacatcg aagacgagaa cttcggcctg
     1801 accgaggtgc ccacaggaac gtgggccggc ttcgtcttca tcaacctcga tcccgactgc
     1861 gagccgctgg aaagctacct ggaagttctt cccgagcact tcaagaactg gaagctcgag
     1921 gaccgcttcg tggccctgca cacccgcaag atcctgccgg gcaactggaa gatgtgcatg
     1981 gaggcgttcc tggaggccta ccacgtgctc gccactcatc ccgaaggcat tcacagcgcc
     2041 ggcgacgcca acgccgacta cgacatcttc ggccgtcacg tcacccgctt cgtgcacacc
     2101 atcggctacc cgtcccccca cgtcgcctcg gaggtggacg agcgcaagct gttcgcggcc
     2161 ctcggccaca acccggacga cctgcccgat ggcatgagcg cccggcagcg ccacgcggac
     2221 atcatgcgtg agcagcttgg gcgcgagtgg gacgtggatc tgagcgggct ctccaacagc
     2281 gagatgctgg attccatcga gtacttcgtc ttccccaacg cctgcttctt ccccggcatc
     2341 aacatccgcc tggtgtaccg cttccggccc atcgacgtgg accacaccat gcacgagatc
     2401 ctgctgctgc agcccgtgcc ggagcacggc gagcggccca agccggccga gcccatcgac
     2461 ctgaccatcg acgagtcata cgagaaggcc aacgaggtct ccctcggcgg gtttgcgctg
     2521 gccatggtct tggaccagga cacggagaac tttcaccgtc agcgcgcggg catcaaggcc
     2581 gcacagaggg cgggtgtgcg aacggccgcc gtcctggcca attaccagga gtcccggatc
     2641 cgccggctgc acatgacggt ggacgagtac atggcgggtt agtcaggttc gcgagttcga
     2701 ctacatcatc atcggagcgg gcaccgccgg ctgcgtgctg gcgaaccgtc tgagcgctga
     2761 caaagatgcc gacgtcctgc tgctggaagc cggcggcaag gacgactact tctggatcga
     2821 catcccggtc ggctacctgt acaccatcaa caacccgcgc acggactggt gctatcaggt
     2881 ggagcccgag cccggcctca acggccgccg catcaactac gcccgcggca aggtcctcgg
     2941 cggctgctcg tccatcaacg ccatgatcta catgcgcggt cagcgcggcg actacgatca
     3001 ctgggctcag ctcggcaatc ggggctggtc gtgggacgac gtgctgccgg tgttcaagcg
     3061 ctccgaggac taccagcacg gcgcgggcga gtttcacggc gcgggaggcg agctgcgcgt
     3121 cgaggagcgg cgcgtcgact gggagattct ggatgcctgg cgggatgctg cccaggagtg
     3181 cggcatcccc aagatcgagg agttcaaccg cggcgacaac ttcggcaatg cctactttca
     3241 ggtgaaccag cggcgcgggg tgcgctggag c
//
LOCUS       DBNHKV010000194         5587 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1231314, whole genome shotgun sequence.
ACCESSION   DBNHKV010000194 DBNHKV010000000
VERSION     DBNHKV010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1231314"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="AC16PP_06150"
     CDS             complement(<1..1301)
                     /locus_tag="AC16PP_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6481246.1"
                     /translation="MSFKRALVVDDSKSARLSLKKLLEQHHLEVDEAFSGEEALDFIK
                     DHSVDVIFMDHTMPGMDGLEAVAAIKSNPKTATIPVMMYTTQEGEVYVGQARALGAVE
                     VLPKQVQPGVLFEMLEKLGLVTDRRTEDQPVSMERRGQEPEAEETDLDREYERQALGM
                     SVQALVTRILEDQHLKLRTDILRSQRHFAKQVAAEIGKAQAESSAAEGAGEQPARSVW
                     TFLRSAAVVALFAGAGLLYHQTQTSIQTAVDRIDSAETLMSDTTRQISRELADEPDET
                     EGDEVARGNLLAALQWAMNLGSRHAFEEPAFSSRRVEELRDLLTNLATAGFSGTVRLE
                     SHLGEFCLVPDGAGGFTLADPELPVQACMLIGHPQEESTFVAERQSLDFVAFLQSTPL
                     LRASGIKVELVAKDRDQSARRYDFPSAVASAGEWNRIAELN"
     gene            1475..2314
                     /locus_tag="AC16PP_06155"
     CDS             1475..2314
                     /locus_tag="AC16PP_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6481247.1"
                     /translation="MLYGIKTSPQLTTWHDMLDVWRAADGMEVFHSAWNFDHFYPINV
                     PDTSGPCMEAWTTLTALAQATERIRIGCMVSGVVYRHPAVLANMIASLDIISDGRLEV
                     GLGAGWNEEECDAYGIELGTLTERFDRFDEACAVIHGMLSVPTTDFAGRYFTLTAARC
                     NPPPLQQPHPPICIGGNGERRTFPNVVRWAQHWNYPGFDVDGFVAKRDVLWDQCRAVG
                     RDPGEIMTSAHQPVDLDDLDDLRRRVTAFREAGLGLLIFYLPPPHRAPALEPIAEIAA
                     QVG"
     gene            complement(2311..3420)
                     /locus_tag="AC16PP_06160"
     CDS             complement(2311..3420)
                     /locus_tag="AC16PP_06160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6481248.1"
                     /translation="MTSSPVNPLRSDEHPFKLAVFGINVSGGCTMTRAPGTLEVNWPE
                     TRQVALAAEQAGFDALIPVARWKGMGGAVNFNHRSWEPFTLASALAAITERIALFSTC
                     HVPTVHPVRLAKESATVDQISGGRFCLNIVAGWNEREIGMFGVQQKPHDERYDVADDW
                     IHLTKRLWTETQEFDHQGPYFSAPGAYSEPKPVQAPHPLIMSAGNSPRGLAFAAEHAD
                     LNFVVAADLDKAGESASAVRAKAAEQGRSIKVFGQGYIVCRSSEAEARAARDRYVHEL
                     GDWEGVRNLLDVLIPNSQSALGDEWEAMAANMIAGYGALPLVGTPDQVVDGMQAFSDA
                     GLDGMTLSWLDYPAGIEQFEKELRPRLVEAGLRAR"
     gene            complement(3417..4406)
                     /locus_tag="AC16PP_06165"
     CDS             complement(3417..4406)
                     /locus_tag="AC16PP_06165"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6481249.1"
                     /translation="MSLASAQTIDVHAHAVLEETMGAAGEHGPELGREAAPRFRVGNY
                     ELHGVRYRGNPFMDPDLRLDAMDAAGIDYQVLSPNPLTYFHFIDAGAARDFCRIHNDT
                     LSAIVGERADRLGAFAALPMQDVDFAIEETERAVRELGMYGPYIGTDFGRPLNDPALD
                     PFYEKLIELDVPLFIHPAPAGIDGPPGDPNLKQFDLDIIIGFAAQETIATCTLIYGGV
                     LERHPKLDVCLSHGGGATGYAFGRMASAAKKRPWASDSLRQDGAFEALLQRLWFDVHV
                     HDPDALALLHKKVNPDHLVFGTNFAGWDQQPGDVRHDAAPYTENARRLLRATR"
     gene            4492..5265
                     /locus_tag="AC16PP_06170"
     CDS             4492..5265
                     /locus_tag="AC16PP_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6481250.1"
                     /translation="MTSSDWGRVRPADIPWKQFDDPHGRPTTPVRVLKGGDPAVLEVR
                     FPPGYVAGLHWHPYDTLYIVTRGSMQIGDEGVYRPGDIRWVRAGHVYGPEAAGAEGVD
                     FFLVSLGGEIELNWADLVDVPERLRERLETSGEPWGRVNLQEVPWQDFEDPAGRPTQP
                     VQVLLDDDPYVLRTRFSPDYVAGDHWHDFDTLYFITSGSMRFGDEGWYEPGEVRWVRG
                     GHSYGPEEPGEGGVEFILVSCGGPVNLHWSDLEPPPSKP"
     gene            5278..>5587
                     /locus_tag="AC16PP_06175"
     CDS             5278..>5587
                     /locus_tag="AC16PP_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481251.1"
                     /translation="MDLLNDHTLTDPEVMRDPYPFYRALHAADARVVQVPGVGFWIGR
                     MKDVREVSRDTVVYSNSYFGEEGPLPTGVNPDPLQQDVQAIFASGPPVKNALWTTDPP
                     V"
BASE COUNT          932 a         1762 c         1848 g         1045 t
ORIGIN      
        1 ttgttcagct ccgcgattct gttccactcg ccggcgctgg ctaccgccga agggaagtcg
       61 tagcgtctgg cagactggtc gcggtccttc gccaccagct ccaccttgat gccggacgcc
      121 cgcagcagcg gcgtgctctg aaggaaggcc acgaagtcga gcgactgcct ttcggcgacg
      181 aaggtcgatt cctcttgcgg gtggccgatg agcatgcagg cctgaacagg cagctccggg
      241 tcggccaggg tgaagcctcc ggctccatcg ggaaccaggc agaactctcc gagatgagat
      301 tccaggcgaa cggtgccgct gaagccggcg gttgccaggt tggtcagcag gtcgcgcagt
      361 tcttcgacac gccgtgagct gaaggccggc tcttcgaagg cgtggcggga gccgaggttc
      421 atggcccact gcaaagctgc gagaaggttg cctcgggcga cttcgtcccc ctcggtttcg
      481 tcgggctcat cggccaactc gcgcgaaatc tggcgggtcg tgtcgctcat gagcgtctcg
      541 gcggagtcga tacggtccac ggcggtctga atgctggtct gggtctggtg atacaggagc
      601 ccggcaccgg cgaacagagc aaccaccgcc gccgaacgca ggaacgtcca cacagatcgg
      661 gcaggttgct ctccggcacc ctcggccgct gaggactccg cttgggcttt gccgatctcc
      721 gctgcgacct gcttggcgaa gtgacgctgg ctccgcagga tgtcggtgcg cagcttcagg
      781 tgctgatcct ccagaattcg cgtcacgagt gcctgcacgg acatgccgag cgcctgccgc
      841 tcgtattccc gatccagatc cgtctcttcc gcctccggtt cctggccgcg ccgctccatt
      901 gagaccggct ggtcctcggt cctgcggtcg gtcaccaggc ccagcttctc gagcatctcg
      961 aacagcacgc cgggttgcac ctgcttcggc aggacttcca ccgcgccgag agcgcgggcc
     1021 tggccgacgt agacctcccc ttcctgggtg gtgtacatca tgacgggaat ggtggccgtt
     1081 ttcggattgc tcttgatggc cgcgaccgct tcgagaccat ccatgccggg catggtgtgg
     1141 tccatgaaga tgacgtcgac ggagtggtcc ttgatgaagt cgagcgcttc ttcgccggaa
     1201 aaggcctcgt cgacctccag gtggtgctgc tcgagcagct ttttcagcga aagccgcgcg
     1261 gatttcgaat cgtcgacgac cagcgcacgt ttgaatgaca tagtggcttc cctgcacttt
     1321 caggcgaggt agtcagcgcg aagggtagcc cttttgcggg cccaagcctg tgatggactt
     1381 cgctttcgcg ccggatcggg acgcacggtg taattcttcg tcggcggttc tatcatctgc
     1441 cccgtcgtca tcggatcggg aagggggttg aaagatgctt tacggaatca agacctcccc
     1501 gcagctcact acctggcatg acatgctcga cgtctggcgg gcggccgatg ggatggaggt
     1561 attccactcc gcctggaact tcgaccactt ttatcccatc aacgtcccgg acacctcggg
     1621 gccctgcatg gaagcgtgga ccacgctcac cgccctggct caggcgaccg agaggatacg
     1681 catcggctgc atggtgagcg gcgtcgtcta tcgccatccc gcggtgctgg ccaacatgat
     1741 cgcctccctc gacatcatct ccgacggtcg gctggaagtc gggctcgggg caggatggaa
     1801 cgaggaggag tgcgacgcct acggaatcga gctgggaacc ctgactgagc gcttcgaccg
     1861 tttcgatgag gcctgtgcgg tcattcacgg catgctgagc gttccgacga cggattttgc
     1921 gggacgatac ttcacgctga ccgccgcccg ctgcaatccg ccgccgcttc agcagccgca
     1981 cccgcccatc tgcattggtg gcaacggcga gcggcgtacg ttccccaacg tcgtgcgctg
     2041 ggcgcagcac tggaactacc cggggttcga cgtcgacgga tttgttgcga agcgggacgt
     2101 gctgtgggat cagtgtcggg ccgtaggccg agacccaggc gagatcatga cgtccgccca
     2161 tcagccggta gatctggacg atctggacga cctgcgccgg cgcgtcacgg ccttccgcga
     2221 ggcgggcctg ggattgctga ttttttacct gccgccgccg caccgggcgc cagctctcga
     2281 gcccatcgcc gagatcgctg cacaggtggg ttagcgggcg cgcaacccgg cctcgacgag
     2341 ccggggtcgg agctcctttt cgaattgctc gatgccggct ggatagtcga gccagctgag
     2401 cgtcatccca tcgagccctg cgtcgctgaa ggcctgcatg ccgtcaacga cctgatccgg
     2461 cgtgcctacc agcggaagcg cgccgtagcc ggcgatcatg ttggcggcca tggcctccca
     2521 ctcatcgccc agcgcgcttt gagagttggg gatgaggacg tcgagcaggt tgcggacgcc
     2581 ttcccagtcg ccgagctcgt ggacgtagcg gtcccgggct gccctggctt cggcctcgct
     2641 ggaacggcaa acgatgtaac cctggccgaa taccttgatg gaacgcccct gctcggctgc
     2701 cttcgcccgg accgcgctgg cactttcccc tgccttgtcg agatcggccg cgacgacgaa
     2761 gttgaggtct gcgtgctcgg cggcgaatgc cagacctctg gggctgttcc cggcgctcat
     2821 gatcagaggg tgaggcgcct gcacgggctt gggctcggag taggcgccag gtgctgagaa
     2881 atagggaccc tgatggtcga actcctgagt ttccgtccac aatcgtttgg tcagatgaat
     2941 ccagtcgtca gcgacgtcgt agcgctcgtc gtggggtttc tgctgcacac cgaacatgcc
     3001 gatctcccgc tcgttccagc ccgctacgat gttcaggcag aacctgccgc cggagatctg
     3061 atccacagtt gccgattcct tcgccagacg cacgggatgc acggtgggca cgtgacaggt
     3121 ggagaagagc gcgatccgct cggtgatcgc cgccagcgcg cttgccagcg tgaacggctc
     3181 ccagctgcgg tggttgaagt tcaccgcgcc gcccatgccc ttccaccgcg ccacgggtat
     3241 cagcgcatcg aaccccgctt gctcggcggc cagcgccacc tggcgggtct cgggccagtt
     3301 cacttccagg gtgcctggag cccgggtcat cgtgcacccg ccgctcacgt tgatgccgaa
     3361 cacggcgagc ttgaaggggt gctcgtcgct gcgcagcggg ttgacgggcg agctggtcat
     3421 cgcgttgcgc ggagcaggcg acgcgcgttc tcggtgtacg gcgcggcgtc gtgacggacg
     3481 tctcccggct gctggtccca gcccgcaaaa ttggtgccga acacaaggtg atcggggttc
     3541 accttcttgt gcagaagggc cagagcgtcc ggatcgtgca cgtgaacgtc gaaccacaga
     3601 cgctggagca gagcctcgaa cgcgccgtcc tggcgcagcg agtcggatgc ccaggggcgc
     3661 tttttcgccg cgctcgccat ccttccgaag gcgtatcccg tggccccgcc accatgactc
     3721 aagcagacgt ccagcttcgg atgacgctcc agcaccccgc cgtagatgag cgtgcaggtg
     3781 gcaatcgttt cctgcgccgc gaagccgatg atgatgtcca gatcgaactg cttgaggttg
     3841 ggatcccccg gcgggccatc gatgcccgcg ggggcggggt ggatgaaaag aggaacgtcc
     3901 agctcgatga gcttttcgta gaaaggatcg agcgcggggt cgttcagcgg ccgtccgaag
     3961 tcggtgccga tgtagggacc gtacatgccg agctcgcgca ccgcccgctc cgtctcctcg
     4021 atggcgaagt ccacgtcctg catgggcagc gcggcaaacg cgccgagacg gtctgcccgc
     4081 tcgccgacga tggcagacag ggtgtcgttg tggatcctgc agaaatccct cgcggcgccg
     4141 gcatcgatga agtgaaagta ggtgagcggg ttgggtgaca ggacctggta gtcgattccg
     4201 gccgcgtcca tggcgtcgag gcgcagatcc gggtccatga acgggttgcc gcggtagcgc
     4261 acgccgtgca gctcgtagtt gcccactcgg aagcgcgggg cagcctcgcg accgagctcc
     4321 gggccgtgct cgcccgcggc ccccatggtc tcttccagaa cggcgtgggc atggacgtcg
     4381 atggtctggg cgctcgcaag gctcatgtgt cgtttcctcg ggctgctgaa gtgggttcac
     4441 tcaccgaaac cagagcgatg atggacccgg tcggatggta agatcaacgt catgacgagt
     4501 tctgattggg gtcgcgtgcg gcccgccgac ataccctgga agcagttcga cgatcctcac
     4561 ggacgaccga ccaccccggt tcgcgttctc aaagggggcg accccgccgt tctcgaggtc
     4621 cggttcccgc cgggttacgt tgccgggctg cactggcacc catacgacac cctctacatc
     4681 gtcactcgag gatcgatgca gataggtgat gaaggtgtgt atcgcccggg agacattcgc
     4741 tgggtacggg cggggcacgt ttacgggccg gaggccgccg gcgccgaagg cgtggacttt
     4801 ttcctggtga gcctcggcgg cgagatcgag ctcaactggg ccgacctggt cgacgtgccg
     4861 gagcggttgc gcgaacgact ggagacctcg ggcgagccgt gggggcgggt aaaccttcaa
     4921 gaggtgccgt ggcaggactt cgaggatccc gccggacgac ccacccagcc ggttcaggtt
     4981 ctcctggacg atgatcccta cgtcctgcga acccgattct caccggatta cgttgccggc
     5041 gaccactggc acgatttcga cacgctttac ttcatcacgt ccggctcgat gcggttcggc
     5101 gatgagggct ggtacgagcc tggtgaagtc cgctgggtgc ggggcggtca cagctacgga
     5161 ccggaagagc ctggagaagg cggggtggag ttcatcctcg tcagttgcgg cggccccgtc
     5221 aatctgcact ggtcggatct ggaaccgccg ccctcgaaac cctaggacgc acaagagatg
     5281 gatctgctga atgaccacac cctgaccgac ccggaagtga tgcgcgaccc ctatccgttc
     5341 tacagggcgc ttcatgctgc cgatgcgcgg gtggtgcagg tgcccggagt cggattctgg
     5401 atcggccgca tgaaggacgt tcgcgaggtt tctcgcgaca ccgtcgtgta ctccaacagc
     5461 tatttcggag aggagggtcc gttaccgaca ggcgtgaacc cggatccgct ccagcaggac
     5521 gttcaggcaa tatttgccag cggaccgccc gtaaagaacg cgctgtggac gacggatccg
     5581 cccgttc
//
LOCUS       DBNHKV010000195        13254 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_735389, whole genome shotgun sequence.
ACCESSION   DBNHKV010000195 DBNHKV010000000
VERSION     DBNHKV010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13254)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13254
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_735389"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="AC16PP_06180"
     CDS             complement(<1..981)
                     /locus_tag="AC16PP_06180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6481252.1"
                     /translation="MTIRSYDEDRDLKAIERIWFECGWLENSRQAAYLKDFLAAGDCL
                     TATIDDTAECAVHVSPGALQYVDEQLELCAVTAVTTSRVARKQGFARELTARQLARGA
                     DDGAEIAALGMFEQGFYDQLGFGTGSYEHEFTFDPATLTVDVPFRPPSRLDRGNWRDI
                     HASLAKRLRGHGGCVLRPSYLIKAELGFSEDGFGLGYFDGSDLTHFFWGEPRGEHGPY
                     RIHFLAYRDAEQLMELLALMRSLGDQVSSIVLLEPPEVQLQDLLDRPFRARRNTRASA
                     HANQHRSIAFWQARVLDVEACVAVRHWPGDEVYFNLVLTDPLSEILAGEAW"
     gene            complement(1016..1972)
                     /locus_tag="AC16PP_06185"
     CDS             complement(1016..1972)
                     /locus_tag="AC16PP_06185"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6481253.1"
                     /translation="MELDYSNIDPELRPALDFLPAELDLITRETLHATRAVLAAAPAP
                     DVETDVEVTEQIIAGPGGDLRLLIYRSPVAEGGPGVLWLHGGGYILGAVDDDRARLVA
                     ESLGCTAVSVDYRLAPEHPFPAAVEDAYAALCWLAGNAGALGVDAERLSIAGLSAGAG
                     LTAGLALLNRDRGGPAVAFQLLLSPMLDNLHDTASGRIVNHPTWSRQTSLNAWEMYLD
                     GTPGLDASPYAAPSRATDLSGLPEAYVSVGAEDLFRDECIDYARRLHEHGVATELAVF
                     PGMYHVAENFAPQARVCQRLHGSYLRALADGLGVSRGAADGS"
     gene            complement(2063..2815)
                     /locus_tag="AC16PP_06190"
     CDS             complement(2063..2815)
                     /locus_tag="AC16PP_06190"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6481254.1"
                     /translation="MSQVQPIRPAATVILVRDAADGFEILMLKRTSGAAFAGGMFVFP
                     GGRVDGDDHLHRYDEHRVGPSDVQQAQQRALGAEWRGYWIAGIRESFEEAGLLLAYDR
                     AGELLAYDDDAVEERFQSYRGPLHAGQVSLETICRQEGLRLAVDRIHFHSRFVTPLGR
                     PRRFDTRFFIAEAPPGQTGRHDDKETVDSIWISPGEALARHAVEEFGLMGVTKLQLEG
                     LAGHRSKADVLAAAARRADFPTRRPVLPPSER"
     gene            2910..3824
                     /locus_tag="AC16PP_06195"
     CDS             2910..3824
                     /locus_tag="AC16PP_06195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6481255.1"
                     /translation="MKLSVEFPSVAYREGPEGIIRLAKAIEDIGYDQLDMFDHVVMGY
                     PTETRAAPIYPPQMPIMEALMVLAYAAAVTTRIGLGTEVLVLPQRQPVLVAKQISTLD
                     TLSGGRVRLGVGVGWQASEYDALEESFANRGRRMDEAIRLLRAYWSEEKVEFAGEYYT
                     SHAMAMEPKPPQGGQLPIWIGGLAPAALRRVGELGDGWLATAFTDPDRARAAVATIHR
                     HAEDAGRDPAAIGLQQMLDVPPRDDSGKGFYADLDQVEARALAVKEMGFEWGALNATA
                     IFQSGARSVDAMIDTLGALHERLRAATA"
     gene            3872..4138
                     /locus_tag="AC16PP_06200"
     CDS             3872..4138
                     /locus_tag="AC16PP_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HIR6481256.1"
                     /translation="MSSQRDDHAYWRANLKLGAVLLGIWFVVSFGFGILLVEPLNRIP
                     LFGFKLGFWWAQQGAIFVFVILIFVYSRLMKRIDRRYGVHDDED"
     gene            4154..5926
                     /locus_tag="AC16PP_06205"
     CDS             4154..5926
                     /locus_tag="AC16PP_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR6481257.1"
                     /translation="MDVQLLTYLFVGLSFALYIGIAIWSRAASTREFYVAGGHVHPVA
                     NGMATAADWMSAASFISMAGIIAFMGYDGTVYLLGWTGGYVLLALLLAPYLRKFGEFT
                     VPDFIGTRYYSNTARVVAVVSLIVISFTYVAGQMRGVGIVFSHFLGVEIELGVVIGMA
                     VVFMYAVLGGMKGITYTQVAQYCVLIFAYLVPAIFISMLITGIPIPQIGLGAAVNDGS
                     GVSVLARLDATLVELGFGPYTQGSKSTIDVFAITMALMIGTAGLPHVIVRFFTVPKVS
                     DARKSAGYALLFIALLYTTAPAVGAFARLNFVETVHGTGYADVAGWFKSWEDVGLIGW
                     LDKNEDGAIQYAPGAPFEGKPEFDGVRGPDGQRLIRNAPTGNANEVYVDRDIMVLANP
                     EIAALPPWVIALVAAGGLAAALSTAAGLLLVISTSVSHDLIKKTMARGISDRQELLYA
                     RIAAALAIAVAGYLGINPPGFVAQVVAFAFGLAAASLFPAILLGIFSKRMNREGAIAG
                     MVVGLTFTFAYIVYFKFVAPGDNNAAHWWLGISPEGIGMVGALANFATAAIVSRFASP
                     APEDVQQLVEKIRIPRGVETPHTH"
     gene            complement(5959..8151)
                     /locus_tag="AC16PP_06210"
     CDS             complement(5959..8151)
                     /locus_tag="AC16PP_06210"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HIR6481258.1"
                     /translation="MSATKRVSRGKLSVAAELDDLVSQRIAPGTGVDAEAFWAGFESI
                     VDDLAPRNRALLARRDELQATIDEWHEDRRGRPLDAQEYRAFLEQIGYLVPEPAPFKI
                     LTADVDDEIARIAGPQLVVPVMNARYALNAANARWGSLYDALYGTDVLPEEPGREKGT
                     SYNPARGELVVERAARFLDETVPLAGASHSEVIAYGIGDGPGGRRLRTILDNGGNTGL
                     ENPEQFVGFIDEDDPSTLLLVHNGLHIEIQIDRSHPVGAAHPAGVKDVVLESAVTTIQ
                     DCEDSVAAVDAEDKCVVYGNWLGLMRGDLEEVMDKGGRAVTRRLNPDRTYVGLDGQPL
                     TLHGRSLMLVRNVGHLMTTDAVLDGAGNEIPEGILDAAVTAFAAMHDLLGTGSVRNSR
                     AGSVYIVKPKMHGPEEVAFTCDLFSRVEQLLGLGPNTLKVGIMDEERRTTVNLRACIF
                     EARERVVFINTGFLDRTGDEIHTSMQAGPVLPKEAIKAEPWIQAYEDANVDAGLAAGF
                     SGVAQIGKGMWPKPDEMKEMLAAKIAHPQAGANTAWVPSPTAATLHVTHYHQVSVFER
                     QQALAAREPASLDDILRIPLLGGRNLSAEEVQAELDNNAQGILGYVVRWIDHGVGCSK
                     VPDINDVGLMEDRATLRISSQHIANWLHHGICSREQVLGTMQRMAAVVDGQNAGDAAY
                     RNMAPDFDASVAFQAACDLVFKGLEQPNGYTEPVLHARRREAKARFGS"
     gene            complement(8236..9438)
                     /locus_tag="AC16PP_06215"
     CDS             complement(8236..9438)
                     /locus_tag="AC16PP_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6481259.1"
                     /translation="MDFEFSKEDQAFREEVRAFLEEKVTPDMRQAVRKTTTVFADKEL
                     AMRWQAILVEKGWAVPAWPAEHGGTGWSVTQKYIWGEECARASAPGLIPLGLRMLAPV
                     IFKYGTEEQKNYYLPRMLSGEHYWCQGYSEPGSGSDLSSLKTSAVKDGDDYIVNGTKI
                     WTTHAQFADHIFCLVRTDTGVRPQAGITFLLIPMDTPGIKVEPIITMAGDHEVNQVFF
                     DDVRVPQANRVGPENDGWNVAKYLLEFERGGGGAAVRLKESLKELRNIASSELADGEP
                     LMDDPGFRNRISELEIRTSALEFAELASLARMSRGESPGTASSLAKNTSVEIDQGILT
                     LKLEAVQHYAMPHANKISLDGHNEPWPGPDHAETVTPRYLNVRAASVYGGSKEVQKNI
                     IAKAVLGL"
     gene            complement(9586..10176)
                     /locus_tag="AC16PP_06220"
     CDS             complement(9586..10176)
                     /locus_tag="AC16PP_06220"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6481260.1"
                     /translation="MSRLQKIEPEDWAPELKAAINPENLTPLEQGLTRFFAHRPELTL
                     GLMQFGGALKTKRTLPDKLIELVRLRVAFFNQCRSCMAIRYTDALNDGVTEDLVCSLE
                     KPAEAGNLTDAEKAAIRFGELMATDHLAIDDAVYDELRKHFSESEIVELGMHVAFFVG
                     FGRLAATWHMVEELPAAFQDDSRPLSPWNEESIVVR"
     gene            complement(10173..11534)
                     /locus_tag="AC16PP_06225"
     CDS             complement(10173..11534)
                     /locus_tag="AC16PP_06225"
                     /EC_number="5.4.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR6481261.1"
                     /translation="MTTVAEARAELAARTAGSQEISEARAEILNSGLAANLEMPHPIF
                     IESGEGPFVFDADGNRYIDTSVGFGLHILGHRHPLIEQAIKDRTEKGWMFGIHSTSQM
                     KLAELLHEASPCAERVVFCNTGSEATMYAIRAARGYSGKEKIALFDGFYHGAHDYGMW
                     LTDPTSPREAPRKLPMGRGIPRALADLTLLLPYRHEAAFDLIRRHADELALVMVEGVQ
                     SSNPQPGAGEFLCELEAVCREAGVLFAVDEVITGFRVAYGGAQERFGVRPDLATYGKI
                     LGGGLPVGAITGRADVMEVFTGLGASNGVFSGGTFSGNPLTMEAGAAAVGYLRDHPDV
                     YRGLEQAGERLAGAVNGFCQERQIPAQMKSVGSMFHMFFQREPIRSMRDVTGASVAAE
                     KAFYLHALNRGVLVPGTQRAFLSAAHSDAEIDTLIDVFQTSLADVQAEGLFSARTDKG
                     EMR"
     gene            complement(11531..12496)
                     /locus_tag="AC16PP_06230"
     CDS             complement(11531..12496)
                     /locus_tag="AC16PP_06230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6481262.1"
                     /translation="MAKPFRFGFQTFNAESGQSWADKARKAEDLGYSSFHLADHLLGP
                     GPALTAANHPIQNVAAIPAMAFAAAVTSTINIGCRVFCIDYHLPVVLIKSAMTIDMLS
                     NGRLELGLGAGWVKSEYEAVGIDFDEPRVRIDRLADVIAGVKAFRGEDLVDVHNDTLS
                     WKDFEGLPKPVGRAPIMVGGGSPRVLRLAGREADIVSLNFNNRMGMIGPDGVQMSSEQ
                     ETRKKIGWIREGAGDRFDDLEIEIGAYFTVVTDDAKPVVAGMAQMFGMSEDEMLRHPH
                     GLFGSVDAICDELQRRREEFGISYFTVGEDNLEAFAPVVARLSGK"
     gene            complement(12523..>13254)
                     /locus_tag="AC16PP_06235"
     CDS             complement(12523..>13254)
                     /locus_tag="AC16PP_06235"
                     /inference="COORDINATES: protein motif:HMM:NF018038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR6481263.1"
                     /translation="IDVYMVANGLYHDIDYARSELLKLLGEHENVRVKVAANYSDIEG
                     IAASDFLITYCCHLIPSEEEQVALRDYVNSGKRWIALHGTNAILEFVEGGVDCPETAP
                     IFMETLGSQFMAHPPIQPFPVKVSDPEHELVAGIGEFETDDELYICKYHGEHHALLHT
                     NFTGSCDGFINRDWPDDEPRVMYYLRKLGDGEVLYNCLGHCRGHYDMRPVTDYYPAIE
                     RGSWDKPEYYELLRRSIKYCIDRVV"
BASE COUNT         2170 a         4484 c         4298 g         2302 t
ORIGIN      
        1 ccaggcttct cctgccagga tctcgctcaa ggggtcggtg agcacgaggt tgaagtagac
       61 ctcgtcgccc ggccagtgcc gaaccgccac gcaggcctca acgtccagca cccgcgcctg
      121 ccagaacgca atggaccggt gctggttggc atgggcggaa gccctggtgt tgcggcgcgc
      181 gcgaaatggc cgatccagga gatcctgcag ctgcacttca ggtggctcca gcaggacgat
      241 ggaggacacc tgatcaccga gggagcgcat cagcgccaga agctccatga gctgctcggc
      301 gtcgcggtag gccaggaagt gtatgcgata gggtccgtgc tcgccgcggg gttcgcccca
      361 gaagaagtgg gtgagatcgg atccgtcgaa gtacccgagg ccgaacccgt cttccgagaa
      421 gccgagctcc gccttgatca gatacgaagg gcgcagcacg cagccgccgt ggccgcgaag
      481 cctcttcgcc agggaggcat gaatgtcgcg ccagttgcct cgatcgagcc ggctcggggg
      541 tcgaaacggc acgtctaccg tcagggtcgc cgggtcgaac gtaaactcgt gctcgtagct
      601 gcccgtgccg aacccgagct ggtcgtaaaa gccctgctcg aacatgccga gcgccgcgat
      661 ctcggcacca tcgtccgctc cgcgggccag ctggcgcgcc gtgagctcgc gggcaaaccc
      721 ctgcttgcgc gcgacccggc tggtagtcac ggcggtcacc gcgcacagct cgagctgctc
      781 gtcgacgtac tgcaaggcgc cgggggaaac atgcaccgcg cactccgctg tatcgtcgat
      841 ggtggcggtg aggcagtcgc ccgcggcgag gaagtccttg agataagcag cctgacgcga
      901 attttccagc cacccgcact cgaaccagat gcgctcgatg gctttgaggt cccggtcttc
      961 gtcgtagctg cggatcgtca taggcacgca gggcgcggaa aacccggatt atgcgtcagg
     1021 agccgtcggc cgcgccacgc gacaccccca gcccgtccgc cagggcgcgc agatagcttc
     1081 cgtgcagccg ctgacagacc cgcgcctggg gcgcgaagtt ttcagccaca tggtacatgc
     1141 cgggaaacac ggccagctcc gtggccaccc cgtgctcgtg gagccggcgg gcatagtcga
     1201 tgcactcgtc ccggaacaga tcttccgcgc caacggatac gtacgcctca gggagaccgg
     1261 agaggtcggt tgcgcggctg ggagccgcat agggtgaggc gtccagcccg ggcgtgccgt
     1321 ccaggtacat ctcccaggcg ttcagcgacg tctgcctgga ccaggtcggg tggttgacga
     1381 tccggcccga ggcggtgtcg tggaggttgt cgagcatggg ggaaagcaga agctgaaagg
     1441 cgacggccgg ccctccccgg tcgcgattga gcagggcaag ccccgccgtc agccccgccc
     1501 ctgcgctgag cccggcaatg gaaaggcgct cggcgtccac accgagcgcg ccggcgttcc
     1561 cggccagcca gcacaacgca gcgtaggcat cttcaaccgc cgccgggaaa ggatgctccg
     1621 gcgccagacg gtaatctacg gataccgccg tgcacccgag cgattcggca acgaggcgtg
     1681 cccggtcgtc gtccaccgct cccaggatgt agccgccgcc gtggagccac agaacccccg
     1741 ggccgccctc ggcaacggga gaccggtaga tcaggagccg caggtcgccg cctggcccgg
     1801 cgatgatctg ctcggtgact tccacgtccg tctccacgtc gggagcggga gcggccgcca
     1861 gcacagcgcg ggtagcgtgc agagtctcac gcgtgatcag atcgagctcc gcgggcagaa
     1921 aatccagcgc aggccggagt tcgggatcaa tattcgagta atcgagttcc atgtgtttct
     1981 ccagctcgct tagtgctcgc gacggtcatg aacccgcaga tcccgactgc catatcttac
     2041 ctacgctcag ttgatcaagg cctcaccgct cgctcggcgg cagcaccggc cgccgcgtcg
     2101 ggaaatcggc cctgcgcgcg gcggcagcga gcacatccgc cttggaccgg tgtccggcca
     2161 gtccttccag ctgcagcttg gtcacgccca tcaggccgaa ctcctccacg gcgtggcgcg
     2221 ccagcgcttc gcccggcgag atccagatac tatccacggt ttccttgtcg tcgtgccgac
     2281 cggtctgccc cggcggcgcc tcggcgatga agaagcgcgt atcgaaacgt cgcggccggc
     2341 caagcggcgt cacgaaccgg ctgtggaaat gaatgcggtc cactgccagc cgcaggcctt
     2401 cctgccggca gatggtttcc aggctgacct gaccggcatg cagcgggccc cgatagctct
     2461 ggaagcgctc ttcaaccgca tcgtcgtcgt aggccagcag ctcgcccgcc cggtcgtacg
     2521 ccaggagcag gccggcctcc tcgaagctct cgcggatacc ggcgatccag tacccgcgcc
     2581 attccgcacc cagcgcccgc tgctgcgcct gctgcacgtc gctgggcccc acgcgatgct
     2641 cgtcgtagcg gtgcaggtga tcgtcgccgt cgacccgccc gccgggaaag acgaacatcc
     2701 cgccggcaaa agcggcaccg ctggtgcgct tgagcatcag gatctcgaac ccgtccgccg
     2761 catcgcgcac gaggatcacc gttgccgccg gacgtattgg ctgcacctga ctcatcaggc
     2821 ctcccgatcg aaacgcggca ggctatccgg tttttacccg gaccgccaca ggcggtatct
     2881 ttgcccgcct gaccatccgg ggatttcaca tgaagctgag cgtcgagttt ccaagcgtcg
     2941 cctatcggga aggcccggag ggcatcatcc ggctggcgaa agccatcgag gacatcggct
     3001 acgaccagct ggacatgttc gaccacgtgg tgatgggcta ccccaccgaa acgcgcgcag
     3061 cacccatcta cccaccgcag atgccgatca tggaggctct catggtgctc gcctacgcgg
     3121 cggccgtgac caccaggatc gggctcggca ccgaggtgct cgtgctgccg cagcgccagc
     3181 ccgtgctggt ggccaagcag atcagcaccc tggacacgct ctccggcggc cgggtgcggc
     3241 tgggcgtggg cgtgggctgg caggcttccg agtacgacgc cctcgaggag agcttcgcca
     3301 atcgcggccg gcgcatggac gaggccatcc gccttctgcg cgcctactgg tccgaggaaa
     3361 aggtggagtt cgccggcgag tactacacct cccacgccat ggccatggag cccaagccgc
     3421 cccagggcgg ccagctgccg atctggatcg gcgggctggc acctgcggcg ctgcgcaggg
     3481 tgggagaact cggagacggc tggctggcca ccgcgttcac ggacccggat cgcgccagag
     3541 ccgccgtggc gaccatccat cgccacgccg aggacgccgg acgtgacccc gccgccatcg
     3601 gccttcagca gatgctcgac gtgccccccc gcgacgactc gggcaagggc ttttacgcag
     3661 acctggatca ggtggaggcc agagccctgg ccgtcaagga gatggggttc gagtgggggg
     3721 cgctcaacgc caccgccatc tttcagtccg gcgcccgcag cgtggacgcg atgatcgaca
     3781 ccctcggcgc gctgcacgag cggctgcgcg ccgcgaccgc ctgattattg cccgcgcagg
     3841 ccttacaatg gccccccggg aggggtaacc gatgtcatct caacgcgacg accatgccta
     3901 ctggcgggcg aacctgaaac tcggggcggt gctgctcggg atctggttcg tcgtctcctt
     3961 cggcttcggt attcttctcg tcgagccgtt gaaccgcatc cccttgtttg gcttcaagct
     4021 cgggttctgg tgggcccagc agggcgcgat cttcgtcttc gtcatcctca tcttcgtcta
     4081 cagccggctc atgaagcgca tcgatcggcg ctacggcgtc cacgacgacg aagactgacg
     4141 gcagcagcgg ccagtggacg tccagctact cacctatctc ttcgtaggcc tgagcttcgc
     4201 gctctacatc ggcatcgcca tctggtcccg agccgcatcg acccgtgagt tctacgttgc
     4261 gggcgggcac gtccatccag tggccaacgg catggctacc gccgccgact ggatgagcgc
     4321 ggcctcgttc atctccatgg ccggcatcat cgccttcatg gggtacgacg gaaccgtcta
     4381 cctgctcggc tggacgggcg ggtacgtgct gctcgcgctg cttctggcgc cctacctgcg
     4441 caagttcggc gagttcacgg tcccggactt catcggcacg cgttactact ccaacacggc
     4501 ccgcgtcgtc gccgtggtga gcctcatcgt catctccttc acctacgtcg cagggcagat
     4561 gcgcggcgtg ggcatcgtct tctcccactt cctcggggtg gagatcgagc tgggcgtcgt
     4621 catcggcatg gccgtggtgt tcatgtacgc cgtgctgggg ggcatgaagg gcatcaccta
     4681 cacccaggtt gcccagtact gcgtgctgat cttcgcctac ctggttccgg cgatcttcat
     4741 atccatgctc atcaccggca ttcccatccc tcagatcggc ctgggcgccg cggtgaacga
     4801 cggttccggg gtcagcgtgc tggccaggct ggacgcgacg ctggtcgagc tcggcttcgg
     4861 cccgtacacc cagggaagca agagcaccat cgacgtcttc gccatcacca tggccctcat
     4921 gatcggcaca gccggcctgc cccacgtcat cgtccgtttc ttcaccgtgc ccaaggtctc
     4981 cgacgcccgc aaatcggccg gctacgcgct gctgttcatc gccctgctct acaccaccgc
     5041 gccggcggtg ggcgcgttcg cccggctgaa cttcgtggaa accgtgcacg gcacggggta
     5101 cgccgacgtg gccggctggt tcaagagctg ggaggacgtc gggctcatcg gctggctgga
     5161 caagaacgag gacggcgcca tccagtacgc gcccggcgcc ccgttcgagg gcaaacccga
     5221 attcgatggg gtgcgcggcc cggacggcca gcggctgatc cgcaacgcgc cgaccggcaa
     5281 cgccaacgag gtctacgtgg accgcgacat catggtcctg gccaatccgg agatcgccgc
     5341 gctgccgccc tgggtcatcg ccctggtggc cgccggcggc cttgccgccg cgctctccac
     5401 cgccgccggg ctgctgctgg tgatctcgac gagcgtctcc cacgacctga tcaagaagac
     5461 catggcgcgg ggcatcagcg accggcagga gctactttac gcccgcatcg ccgcggcgct
     5521 ggccatcgcc gtcgcaggct atctgggcat caacccgccc ggcttcgtcg cccaggtggt
     5581 tgccttcgcc ttcggtctgg ccgccgcttc cctgttcccg gcgatcctgc tcggcatctt
     5641 cagcaaacgg atgaaccggg aaggcgccat tgcggggatg gtggtgggcc tgacgttcac
     5701 gttcgcctac atcgtctact tcaagttcgt cgcgcccggc gacaacaacg ccgcccactg
     5761 gtggttgggc atctctcccg agggcatcgg catggtgggg gcgctcgcca acttcgcgac
     5821 cgcggccatc gtcagccggt tcgcgagccc ggcgccggag gacgtccagc aactggtgga
     5881 aaaaatccgc atcccgcggg gcgtggagac gccccacacc cactgatcgg ccggcccggt
     5941 accgggccag cgagagggct agctgccgaa gcgggccttg gcctcgcgtc ggcgagcgtg
     6001 cagaaccggc tcggtgtagc cgttgggctg ctccaggccc ttgaagacga gatcgcaggc
     6061 cgcctggaac gccacgctgg catcgaagtc cggcgccatg ttgcggtagg cggcatcgcc
     6121 cgcgttctgc ccgtccacca cggcggccat ccgctgcatg gtcccaagca cctgctcgcg
     6181 gctgcagatg ccgtgatgca gccagttggc gatatgctga gacgagatgc gcagcgttgc
     6241 ccgatcctcc atgagaccga cgtcgttgat gtcagggacc ttggagcaac ccacgccatg
     6301 atcgatccat cgaaccacat agccgagaat gccctgggcg ttgttgtcga gctcggcctg
     6361 cacttcctct gccgacaggt tgcgtccgcc gaggagcggg atgcggagaa tgtcatccag
     6421 gctcgccggc tcccgggcgg ccagcgcctg ctgacgctcg aaaacgctca cctgatgata
     6481 gtgggtgacg tgcagggtag ccgccgtcgg cgagggcacc caggccgtgt tggcaccggc
     6541 ctgggggtga gcaatcttgg ctgccagcat ctccttcatc tcgtccggct tgggccacat
     6601 gcccttgccg atctgggcga ccccgctgaa accggctgcc agcccggcgt ccacgttggc
     6661 gtcctcgtaa gcctgaatcc acggctccgc cttgatggct tccttgggca gcaccggacc
     6721 ggcctgcatg ctggtgtgaa tctcgtcccc cgtgcggtcc aggaaaccgg tgttgatgaa
     6781 caccacgcgc tcgcgggctt cgaatatgca ggcacggaga ttgaccgtgg tgcggcgctc
     6841 ctcgtccatg atgccgacct tgagcgtgtt cggcccaagg ccgagcagct gctccacccg
     6901 ggagaacaga tcgcaggtga acgccacctc ttcgggtccg tgcatcttgg gcttgacgat
     6961 gtaaaccgac ccggctcggg agttcctcac cgagccggtc ccgagcaggt cgtgcatcgc
     7021 ggcaaaggcg gtcaccgccg cgtcgaggat gccctcggga atctcgttgc cggcaccgtc
     7081 gaggacggca tcggtggtca tgagatgccc cacgttgcgc accagcatca ggctgcgccc
     7141 gtgcagggtc agcggctggc cgtctagccc gacgtaggtc cggtcaggat tgagccggcg
     7201 cgtgacggcg cgcccaccct tgtccatcac ttcctcgaga tcgcccctca tcagcccgag
     7261 ccagttcccg tagaccacgc acttgtcctc ggcgtccacg gcggccacag agtcctcgca
     7321 gtcctgaatg gtggtcacgg cggactcgag caccacgtcc ttcacgccgg cggggtgcgc
     7381 ggcgcccacc gggtggctcc ggtcgatctg gatctcgatg tgcaggccgt tgtggacgag
     7441 cagcagagtg ctggggtcgt cctcgtcgat gaagccgacg aactgttccg ggttctcgag
     7501 acccgtgttg ccgccgttgt cgagaatggt ccgtagccgc cgaccgccgg ggccgtcgcc
     7561 gatgccgtag gcaatcacct cgctgtggct ggcaccggcg aggggcaccg tctcgtccag
     7621 gaaccgggcc gcacgctcca ccacgagctc gccccgggcg gggttgtagc tggtgccctt
     7681 ctcccgcccc ggttcttcgg gcagaacgtc ggttccgtaa agggcatcgt aaaggctgcc
     7741 ccagcgcgcg ttggcggcat tgagggcgta gcgcgcgttc atcacgggaa cgacgagctg
     7801 agggccggcg atgcgcgcga tctcgtcgtc gacgtcggcg gtcaggatct tgaaaggcgc
     7861 gggctcgggg acgagatagc cgatctgctc cagaaaggcc cgatactcct gcgcgtccag
     7921 gggacggcct cggcggtctt cgtgccattc atcgatggtg gcctggagct cgtcccgccg
     7981 ggcgagcagc gcccggttgc ggggagcgag gtcgtcgacg atggactcga agcctgccca
     8041 gaacgcctcc gcatccacgc cggtgcccgg cgcgatccgt tgagaaacca gatcatcaag
     8101 ctcggcggcc accgagagtt tgccgcggga cacccgcttc gttgcgctca tcgtcgcccc
     8161 cttttggccc agaagcggcc cggaggaatg ggccccgggc gaagcgagcc agtatagccg
     8221 ccgccgggcg cggggttaca gccccaggac tgccttggcg atgatgttct tctggacttc
     8281 cttggacccg ccgtaaaccg acgccgcgcg cacgttcagg tagcggggcg tgaccgtctc
     8341 ggcgtggtcg ggtcccggcc acggctcgtt gtggccgtcc agcgagatct tgttcgcgtg
     8401 gggcatggcg taatgctgta ccgcctcgag cttcagggta aggatgccct ggtcgatttc
     8461 cacggaagtg ttcttcgcca gggaggaagc ggtgccgggc gactcgcccc gggacatccg
     8521 ggcaagggaa gccagctccg cgaactcgag cgccgaggtg cggatctcca gctcgctgat
     8581 ccggttgcgg aagccgggat catccatcag cggctcgccg tcggccagct cgctggaggc
     8641 gatgtttctc agctccttga gcgactcttt gagccggacg gccgcaccac cgccgccgcg
     8701 ctcgaactcg agcagatact tggccacgtt ccagccgtcg ttctccggcc ctacccgatt
     8761 cgcctggggc actcgcacgt cgtcgaagaa cacctggttg acctcgtggt cgccggccat
     8821 ggtgatgatc ggctccacct tgattcccgg cgtgtccatg ggtatcagca ggaaggtgat
     8881 gcctgcctgg ggacgcacgc ccgtgtctgt gcgcaccagg cagaagatgt ggtcggcaaa
     8941 ctgggcgtgg gtcgtccaga tcttggtgcc gttgacgatg tagtcgtcgc cgtccttgac
     9001 tgcggaagtt ttcaggctgg aaagatcgga tccggacccg ggctccgagt agccctgaca
     9061 ccagtagtgc tcgccggaca gcatgcgagg caggtagtag ttcttctgct cctcggtccc
     9121 gtacttgaag atcacgggcg ccagcatgcg aagcccgaga gggatgaggc cgggggcgga
     9181 cgcccgcgcg cactcttcgc cccagatgta cttctgggtg acgctccatc cggtgccgcc
     9241 gtgctcggcg ggccacgccg gcacggccca ccctttctcc actagaatcg cctgccagcg
     9301 catggcgagt tccttgtcag cgaacaccgt cgtcgtcttg cgcaccgcct ggcgcatgtc
     9361 cggcgtgacc ttctcttcca ggaaggccct gacctcttcc cggaacgcct gatcttcctt
     9421 gctgaactcg aaatccacgg ctacttctcc ttactgtcta cgactgccct agacgccgac
     9481 cctttccggc cttcggctcc atgatgatct ttccgcgccc gtgacgggcg cgtgaagata
     9541 aggacgcaac gcgccgccgc ccggagcggg ccggcagcgc cggtctcaac gcacgacgat
     9601 ggattcttcg ttccagggac tcaagggacg gctgtcgtcc tgaaacgctg ccggcagctc
     9661 ttccaccatg tgccaggtgg ccgccagcct gccgaacccc acgaagaacg ccacgtgcat
     9721 ccccagctcc acgatctcgc tttccgagaa gtgcttgcgc agctcgtcgt agacggcatc
     9781 gtcgatggcc agatgatcgg tcgccatgag ctccccgaac cggatggccg ctttctcggc
     9841 atctgtgagg ttcccggctt cggccggttt ctccagcgag cagaccagat cctcggtcac
     9901 gccgtcgttg agagcgtccg tgtaacggat cgccatgcag ctgcggcact ggttgaagaa
     9961 cgcgacccgc agccgcacga gctcgatgag cttgtcaggg agcgtccgct tggtcttgag
    10021 cgcgccgcca aactgcatca ggcccagcgt aagctcgggc cgatgagcga agaaccgggt
    10081 caggccctgc tccagcgggg tcaggttctc gggattgatc gcggccttga gctcaggcgc
    10141 ccagtcttca ggctcgattt tctgtagtcg actcatcgca tttccccttt gtctgttcgg
    10201 gccgagaaaa ggccctccgc ctgcacgtcg gcaagcgatg tctgaaacac atcgataaga
    10261 gtatcaattt ccgcgtcgga atgagcagcg gaaaggaacg cgcgctgggt cccgggcacc
    10321 agaacgcccc ggttcagcgc gtgcagatag aacgccttct ccgcggcgac gctcgcgccg
    10381 gtgacgtcgc gcatgctgcg gatgggctcc cgctgaaaga acatgtgaaa catgctcccc
    10441 acggacttca tctgggccgg gatctgccgt tcctggcaga agccgttcac cgcccccgcc
    10501 agccgctcgc cagcctgctc gagaccacgg tacacgtcgg ggtggtcgcg aaggtagccc
    10561 accgcggccg ctcccgcctc catggtgagc gggttgcccg agaacgtacc gccggagaac
    10621 accccgttgc tcgccccgag cccggtgaac acctccatca cgtccgcacg gccggtgatt
    10681 gcccccacgg gcaagccgcc gccgagtatc ttcccgtagg tggcgaggtc cggcctcacg
    10741 ccgaatcttt cctgagcacc gccgtaggcg acgcggaaac ccgtgatgac ctcgtcaacg
    10801 gcaaacagaa cgcccgcttc acggcagacg gcttcgagct cgcatagaaa ctcgccggcc
    10861 ccgggctggg gattcgagct ctgaacaccc tctaccatga ccagcgcaag ctcgtccgcg
    10921 tgacggcgga tcagatcgaa ggcggcctcg tgacgatacg gcagcagcag cgtcaggtct
    10981 gccagcgccc gcgggatgcc gcgccccatt ggcagcttgc gcggcgcttc ccgggggctt
    11041 gtcgggtcgg tcagccacat cccgtagtcg tgggcaccgt gatagaagcc gtcgaacaga
    11101 gcgatcttct ccttgccaga gtacccacgg gcagcacgga tcgcgtacat ggtcgcctcg
    11161 gaacccgtgt tgcagaaaac cacccgctcg gcgcaggggc tggcctcgtg caggagctcg
    11221 gccagcttca tctggctggt ggagtgaatg ccgaacatcc accctttctc cgtgcggtcc
    11281 ttgatggcct gctcgatcag cgggtgccga tggccgagga tgtgcaggcc gaagcccacg
    11341 gaggtgtcga tgtagcggtt gccgtccgca tcgaagacga acggcccctc gccgctctcg
    11401 atgaagatcg gatggggcat ctccaggttg gcagcgagcc ccgagttgag aatctcggcg
    11461 cgggcctcgg agatctcctg ggatccggcc gttctggcgg ccagctcggc gcgcgcttca
    11521 gcgaccgtgg tcatttgccg gaaaggcgcg cgaccaccgg cgcaaaggcc tccagattgt
    11581 cctctccgac ggtgaagtag gagatgccga actcctcgcg gcgacgctgc agctcgtcgc
    11641 agatggcatc aacgctgccg aagagaccgt gggggtggcg gagcatttcg tcctcggaca
    11701 tgccgaacat ctgcgccatg cccgcgacca cgggcttggc gtcgtcggtc accacggtga
    11761 agtaggcgcc gatctcgatc tccagatcgt cgaagcgatc gccggcgcct tcgcggatcc
    11821 agccgatctt cttgcgggtc tcctgctcgc tggacatctg cacgccgtcg ggcccgatca
    11881 tgcccatgcg gttgttgaag ttcaggctga cgatgtcggc ctcccgtccg gccaggcgca
    11941 gcacccgggg gctgccgccg ccgaccatga tgggagcgcg tcccacgggc ttgggcagac
    12001 cttcgaagtc cttccaggac agcgtgtcgt tgtggacatc gaccaggtcc tcgccgcgga
    12061 acgccttcac cccggcaatg acgtcggcaa gccggtcgat gcgcacgcgg ggctcgtcga
    12121 agtcgatacc caccgcttcg tactcgctct tcacccagcc ggcgcccaga cccagctcga
    12181 gccggccgtt ggacagcatg tcgatggtca tggcggactt gatcagcacc acgggcaggt
    12241 gatagtcgat gcagaacacc cggcaaccga tgttgatggt gctcgttacc gccgctgcaa
    12301 acgccatggc ggggatggcg gcgacgttct gaatcgggtg gttggctgcg gtcagcgccg
    12361 gccccggccc cagaagatga tcggccagat ggaacgacga gtagccgaga tcctcggcct
    12421 tgcgagcctt gtctgcccag ctctggcccg actccgcgtt gaacgtctgg aacccgaaac
    12481 gaaacggctt tgccatgctc ggctctccct ggcagctgcc cgctagacca cgcggtcgat
    12541 gcagtacttg atggaccgcc gcagcagctc gtaatactcg ggcttgtccc agctgccgcg
    12601 ctcgatggcg gggtaatagt cggtgaccgg gcgcatgtcg tagtggcccc ggcagtgtcc
    12661 gagacagttg tacagcactt cgccgtcgcc cagcttgcgc aggtagtaca tcacccgcgg
    12721 ctcgtcgtcc ggccagtccc ggttgatgaa gccgtcgcag gatccggtga agttggtgtg
    12781 cagcagcgcg tggtgctcgc cgtgatactt gcagatgtac agctcgtcgt cggtttcgaa
    12841 ctcgccgatc ccggccacga gctcgtgctc ggggtcgctg actttcacgg ggaagggctg
    12901 gatgggcggg tgcgccatga actgcgagcc cagggtctcc atgaagatgg gcgcggtctc
    12961 cggacagtcc acgcctccct cgacgaactc gaggatcgcg ttggtgccgt gcagcgcgat
    13021 ccagcgcttg cccgagttga cgtagtcccg cagggccacc tgctcttctt cgctgggaat
    13081 caggtggcag cagtaggtga tcagaaaatc gctggcggcg atgccttcga tgtccgagta
    13141 gttggccgcg accttcaccc ggacgttctc atgctccccg aggagcttga gcagctccga
    13201 gcgggcgtag tcgatgtcgt ggtacagacc gttggcgacc atgtacacgt cgat
//
LOCUS       DBNHKV010000196         8524 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_665375, whole genome shotgun sequence.
ACCESSION   DBNHKV010000196 DBNHKV010000000
VERSION     DBNHKV010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8524
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_665375"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="AC16PP_06240"
     CDS             complement(<1..289)
                     /locus_tag="AC16PP_06240"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HIR6481264.1"
                     /translation="MLQKLRDQTQSFGFKVLAGVMIFVLAVFGFGAFNLFVNSDPEVA
                     SVNGEEITQSELLVAMEREQGRIAAQFGEGFDPSLIDGARLQAAVLDQLIAR"
     gene            complement(394..666)
                     /locus_tag="AC16PP_06245"
     CDS             complement(394..666)
                     /locus_tag="AC16PP_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016782315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR6481265.1"
                     /translation="MNKTELIDRIAESADISKAAAGRALDAAIDAITDSLKQADPVSL
                     VGFGTFTVRDRAARTGRNPQTGAEIQIAAARVPAFKPGKALKGALN"
     gene            complement(802..3231)
                     /gene="lon"
                     /locus_tag="AC16PP_06250"
     CDS             complement(802..3231)
                     /gene="lon"
                     /locus_tag="AC16PP_06250"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR6481266.1"
                     /translation="MVTVADTTRVPVLPLRDMVVYPHGVHPLFVGTESSIKALDAAMA
                     DDKQILLVAKRDAEVESPGAEDLFAVGTVATILQLLKLPDGTVKVLVEGGERARLLQL
                     DQEEDFLVASVELVAEPVVEARESEALVRTLMSQFEQYVKVSKKVPQEVLSSLSSIDD
                     PARLADTVAAQMALKIDDKQGILEAFELKERMDALLVLMDSEIDTQQLEKRIRGRVKK
                     QMEKSQREYYLNEQMKAIQKELGDLEEAPNEFEALEARIEEQGMSKEAREKALAELGK
                     LKLMSPMSAEATVVRSYLDWMLSIPWKKRSRVRNDLAAAQRILDEDHYGLEEVKDRII
                     EYLAVQSRVRKIKGPVLCLVGPPGVGKTSLGESIARATNRKFVRMALGGVRDEAEIRG
                     HRRTYIGSLPGKILQKMAKAGVRNPLFLLDEVDKMGMDLRGDPASALLEVLDPEQNNS
                     FNDHYLEVDYDLSDVFFICTSNSMNIPAALLDRMEIIRLPGYTEDEKLNIAKRYLVPK
                     QMKLNGLSEAELEIGDGAIVHLIRYYTREAGVRGLEREIAKLARKVVKQQSLEGKGRG
                     KGKKGTITVDELQIEDYNGVRKFSYGLAETENQIGIVTGLAWTQVGGELLNIEAVAVP
                     GKGRQTRTGSLGDVMQESIQAALTFVRSRSAILGVRKDFHEKFDLHIHVPEGATPKDG
                     PSAGIGMCTALVSVLTGIPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGVKHVI
                     IPAENERDLKEIPENVKEKLTIHPVKWMDDVLEIALERMPEPRLDEEESSEKPISEVA
                     SQDGELPINPH"
     gene            complement(3345..4625)
                     /gene="clpX"
                     /locus_tag="AC16PP_06255"
     CDS             complement(3345..4625)
                     /gene="clpX"
                     /locus_tag="AC16PP_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR6481267.1"
                     /translation="MSDQSGKGDDSGKLLYCSFCGKSQHEVRKLIAGPSVFICDECVE
                     LCNDIIREEVSEGGAAKESDRLPVPQEIKDILNQYVIGQEHAKKVLSVAVYNHYKRLN
                     YTGKKDDVELSKSNILLIGPTGSGKTLLAETLARLLDVPFTIADATTLTEAGYVGEDV
                     ENIIQKLLQKCDYDVERAQVGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIE
                     GTVASVPPQGGRKHPQQEFLQVDTSNILFICGGAFAGLDKVITDRSDKSGIGFGAQVK
                     GEEDRKSIGETLRRVEPEDLIRYGLIPEFVGRLPVVATLEELDTDALVQILTEPKNAL
                     TKQYTKLFEMEGVEVDFREDALRAVAQKAMERKTGARGLRSILESVLLDTMYDLPSSD
                     SVTKVVIDESVIKGESEPMLMYENVEKAQAAPKE"
     gene            complement(4770..5420)
                     /gene="clpP"
                     /locus_tag="AC16PP_06260"
     CDS             complement(4770..5420)
                     /gene="clpP"
                     /locus_tag="AC16PP_06260"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIR6481268.1"
                     /translation="MSQIGDANIPEITNLGLVPMVVEQSARGERAFDIYSRLLKERIV
                     FMVGPVEDNVANLVVAQLLFLESENPDKDIHLYINSPGGSVTAGLSIYDTMQFIRCDV
                     STMCIGQAASMGAFLLAAGAKGKRYALPNSRMMLHQPSGGGRGVAADIEIQAKEILLM
                     REQLNRLLADHTGQSVERIATDTDRDYWMSPDQAAEYGLVDMVQRPRSDVAKAVNE"
     gene            complement(5417..6784)
                     /gene="tig"
                     /locus_tag="AC16PP_06265"
     CDS             complement(5417..6784)
                     /gene="tig"
                     /locus_tag="AC16PP_06265"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR6481269.1"
                     /translation="MHISVETMSGLERRLTISVPSQTFEGQITERLNRAAQQVRLPGF
                     RAGKVPMKEVRRRFGPAVRAEVAGELMQSSFVEAVRQEELAPAGSPNLEVVKMSPGID
                     FEFTATFEVFPKIELADLSGVHINRPNAQITELDVDEMVERLREQRKEWVAVDRGAED
                     GDRVTIDFAGRIDDEAFEGGSGEDVTFIVGAGQMIEDFDHGVRGMQAGTDGEFQATFP
                     DDYRAEELRGKQATFSVTVKEVTEGKLPELDDEFFAAFGIEEGGVDAFREDVRNNMQR
                     ELDNAVASQVKTQVMDQLNELHTVQLPKAVVKQEIEALKAQMLQQFQMPGTAAGGLPD
                     LPDELFAEQAQRRVAVGLIVSELVSRKSLEADADKVRARIQSIAEQYAEPQQVINWYY
                     GNEDQLRQIEMAVLEEQVIDEVMEQAAVEAVESSYNDIISGRAFAAPEAEPDEEATET
                     SGRIE"
     gene            complement(6834..6909)
                     /locus_tag="AC16PP_06270"
     tRNA            complement(6834..6909)
                     /locus_tag="AC16PP_06270"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6874..6876),aa:His,seq:gtg)
     gene            complement(6983..7059)
                     /locus_tag="AC16PP_06275"
     tRNA            complement(6983..7059)
                     /locus_tag="AC16PP_06275"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7023..7025),aa:Arg,seq:tct)
     gene            complement(7076..7152)
                     /locus_tag="AC16PP_06280"
     tRNA            complement(7076..7152)
                     /locus_tag="AC16PP_06280"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7116..7118),aa:Pro,seq:tgg)
     gene            7325..8176
                     /gene="folD"
                     /locus_tag="AC16PP_06285"
     CDS             7325..8176
                     /gene="folD"
                     /locus_tag="AC16PP_06285"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR6481270.1"
                     /translation="MTASILDGKSLAKSLRADLAAAVQERLESGGRPPGLAVILVGND
                     PASEVYVRHKSRDCEEVGFHSVVQHLSENVTQQDLEERIVALNANPSIDGILVQLPLP
                     AHIDSHRIIDRIDPLKDVDGFHPYNIGRLALRRPVLRPGTPKGIMQLLEVTGVPIRGL
                     DATILGASNHVGRPMGLELLLAGCTVTTAHKFSRDARASARSADILVSAVGKPGLVGG
                     DWIKPGAIVIDVGITRGEDGKLRGDVVFDEAMEVASWVTPVPGGVGPMTRVAMLQNTL
                     AAADGSD"
     gene            8181..>8524
                     /locus_tag="AC16PP_06290"
     CDS             8181..>8524
                     /locus_tag="AC16PP_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6481271.1"
                     /translation="MHRIYISDLHLSDESEPQFATLAALLRHEAPRADAIFILGDLCE
                     AWVGDDDDSPLATALRAALKYAGEQTSLHVMRGNRDFLLGERFAAEIGCCLVEDPCLI
                     DPDTLIAHGDAF"
BASE COUNT         1522 a         2740 c         2477 g         1785 t
ORIGIN      
        1 gcctggcaat cagctgatcc agcacggcgg cctgtagacg tgcgccgtcg atcaggctgg
       61 ggtcaaagcc ttcaccgaac tgcgccgcaa tcctcccctg ctcgcgctcc atggctacca
      121 gcagctcgct ctgggtgatc tcctcgccgt tcacgctggc gacttcggga tcggaattca
      181 cgaacaggtt gaacgccccg aagccaaaca cggccagcac gaagatcatc acgccggcca
      241 ggaccttgaa gccgaagctc tgggtctgat cacgcagctt ctgcagcatt tacgaactcc
      301 ttcagaggac gattaacgcg cctacgcgtc tcggcagcga aaagaaaaaa aggcgcagtg
      361 ctgcgcctta attccgggca agtgttggac ggtctagttc agtgcgcctt tcaacgcctt
      421 gcctggcttg aaggcgggta cgcgggcggc agcgatctgg atctccgcac ccgtctgcgg
      481 gtttctgccc gtgcgcgcgg cgcggtcacg cactgtgaac gttccaaagc ccaccagcga
      541 aaccgggtcg gcctgcttga gcgagtcggt gatggcgtca atggctgcgt ccagagccct
      601 gccggcggcg gctttggaaa tgtcggcgga ctcggcgata cgatcaatta gttcggtttt
      661 gttcacaatc ggcccctcaa tgatgtggtt tgctatcgcc agcatggcct agaaacgctg
      721 ccaattcagg cgtggcgacg ccggttttat accaacacgg ttacgggcct gcaagcttga
      781 aagcccggta aatcgtgggt ttcagtgcgg attgatgggc agctcgccat cctgggaagc
      841 gacctccgag atcggctttt cgctcgactc ttcttcgtcg agccgtggct cgggcatgcg
      901 ttccagggca atctctagaa cgtcgtccat ccacttcacc ggatgaatgg tcagcttctc
      961 tttgacattc tcgggtatct ccttgagatc ccgctcattc tccgccggaa tgatcacgtg
     1021 tttgaccccg ccacgatgag cggccagcag cttctccttg aggccgccga tgggcaatac
     1081 ctggccgcgc agagtaattt cgccggtcat ggcaacatcg gcgcgcacgg ggatgccggt
     1141 cagcacggaa acgagcgcgg tgcacatacc gatgccggca ctgggtccat ccttgggggt
     1201 cgcgccttcg ggaacgtgaa tgtgcagatc gaacttctcg tggaaatcct tcctgacgcc
     1261 gagaatggcc gaccggctgc ggacgaaggt cagcgccgcc tggatggact cctgcatcac
     1321 gtcgccaaga gacccggttc tggtctggcg gccttttccg ggaaccgcca cggcctcgat
     1381 gttcagcagc tcgcccccta cctgggtcca ggcgagaccg gtgacgatgc cgatctggtt
     1441 ctcggtctct gccagcccgt agctgaactt tcgaacgccg ttgtaatctt caatctgcaa
     1501 ctcgtcgacg gtgatcgttc cctttttgcc tttgcctctc cctttgcctt caagagactg
     1561 ctgcttgacc accttgcgag cgagcttcgc gatttcccgc tccaacccgc gaacgccggc
     1621 ttctctcgtg tagtagcgga tcaggtgaac gatggcgccg tcaccgatct cgagctcagc
     1681 ctcggaaagg ccgttgagct tcatctgctt gggtacgaga tagcgcttgg cgatgttgag
     1741 cttctcgtct tccgtgtacc ccggcagacg aatgatctcc atgcggtcca gcaaggcggc
     1801 cggtatgttc atcgagttcg aggtgcagat gaagaacacg tcggatagat cgtagtccac
     1861 ctcgaggtag tgatcgttga aactgttgtt ctgctcggga tccagcacct cgagcagcgc
     1921 gctggcgggg tcgccgcgaa ggtccatgcc catcttgtcg acctcgtcga gcaggaacag
     1981 aggattgcga acaccagcct tggccatctt ctgcagaatc ttaccgggca gcgaaccgat
     2041 gtaggtgcgc cggtgcccgc gtatctcggc ttcgtcacgg acgccgccca gcgccatgcg
     2101 gacgaacttc cggttggtcg cgcgggcaat gctttcgccc agggacgttt tgcccacgcc
     2161 aggtgggccg acaaggcaga gcacgggccc tttgatcttg cggaccctgg actgaaccgc
     2221 gaggtactcg atgatccggt ctttgacctc ctccagaccg tagtgatcct catcgagaat
     2281 gcgctgcgcc gctgccagat cgtttcgaac gcgagagcgt tttttccagg gaatgctgag
     2341 catccagtcc agatagctcc tcaccaccgt ggcttcggcc gacatcggcg acatcagctt
     2401 cagcttgccg agctcggcca gggctttctc ccgagcctct ttcgacatcc cctgctcttc
     2461 gattcgcgcc tccagggcct cgaactcgtt cggggcttct tcaaggtcgc ccagctcctt
     2521 ctgaatggcc ttcatctgct cgttcaggta gtactcgcgc tggctctttt ccatctgctt
     2581 tttgacgcgc ccgcgaatac gcttctccag ctgctgggtg tcgatctccg agtccatgag
     2641 cactagcagg gcgtccatcc gctccttgag ctcgaacgcc tcgagaatcc cctgcttgtc
     2701 gtcgatcttc agcgccatct gcgcggcaac ggtgtcggca agtctcgccg ggtcgtcgat
     2761 gctggacagc gaggagagca cttcctgggg caccttcttg ctgaccttga cgtactgctc
     2821 gaactggctc atcagggttc gaaccagcgc ctcggactcc ctggcttcga caacgggttc
     2881 ggcaacgagc tcgacgctcg cgacgaggaa gtcctcctcc tgatcgagct ggagcaggcg
     2941 agcccgctcc cctccttcaa cgaggacctt gacggtgccg tcgggaagct tcagcaactg
     3001 caggatcgtc gcgacggttc caacggcaaa gagatcttcg gctccgggcg attccacctc
     3061 ggcgtcgcgc ttggcaacca gaaggatctg cttgtcgtca gccatggcgg cgtccagcgc
     3121 cttgatggag ctttccgtgc ccacgaagag agggtgcacc ccatgggggt agaccaccat
     3181 gtcgcgaagg ggaagcacgg gcactcgggt agtatcagct actgtcacca tgaaaaagcc
     3241 ctctgtgaaa gttgtgggca atcagatacg ccataaatgg ctggcgttgc cgaccagcgt
     3301 agtcgccggt ggctaggatg gtgccgctcc gggcaggcct gtcttcactc cttcggagcg
     3361 gcctgcgcct tttcgacgtt ctcgtacatc agcatcggct cgctttcgcc tttgatgacg
     3421 ctctcgtcga tgaccacctt ggtcaccgag tccgaagaag gcaggtcata catggtgtcg
     3481 agcaggaccg actccagaat cgacctcagg ccccgggcgc ccgttttgcg ttccatggcc
     3541 ttctgggcaa ccgcgcgcag tgcgtcctca cggaagtcca cttccacgcc ttccatctca
     3601 aacagcttcg tgtactgctt ggtcagggcg ttcttcggct ccgtgaggat ttggaccagc
     3661 gcgtccgtat ccagttcctc taaggtggcg accacgggca ggcggccgac gaactctggg
     3721 ataagcccgt agcggatcag atcttccggc tccacacggc gcagcgtttc gccgatggac
     3781 ttccggtcct cctcgccttt cacctgagca ccgaatccga tgcccgactt atccgaccgg
     3841 tcagtgatga ccttgtccag accggcgaaa gccccaccgc agatgaagag gatgttcgag
     3901 gtgtcgacct gcaggaactc ctgctgcgga tgcttgcggc cgccctgggg cggcaccgac
     3961 gcgacggtgc cctcgatcag cttgagcagg gcctgctgca cgccctcacc cgacacgtcg
     4021 cgagtgatgg aggggttgtc agactttcgc gaaatcttgt cgatctcgtc gatgtagacg
     4081 atgcccacct gagcgcgctc gacgtcgtag tcgcacttct gcagcagctt ctgaatgatg
     4141 ttttcgacgt cctcgccgac gtagcccgcc tcggtcaacg tcgttgcgtc cgcgatggtg
     4201 aagggaacgt ctagcagtcg cgccagggtc tcggcgagca gcgtcttccc gctacccgtg
     4261 ggcccgatga ggaggatgtt ggacttcgac agctcgacat cgtccttctt gcccgtgtag
     4321 ttcagccgct tgtagtggtt gtagaccgcc acggaaagaa cctttttggc atgctcctgg
     4381 ccgatgacgt actggttcag gatatccttg atctcctgag gtacgggcag gcgatcgctc
     4441 tccttggcgg cgccgccttc cgacacctcc tcacggatga tgtcgttgca aagttccacg
     4501 cactcatcgc agatgaacac cgatggcccg gcaatgagct tgcgaacctc atgctggctc
     4561 tttccacaga aagagcagta gagcaatttg cctgagtcgt cgcccttacc gctttggtcg
     4621 gacatagaaa acctcgtctg ctgcctgtct ccgccgaacc acgccgcacc ctggcgctgc
     4681 gtgcttttga ccttcgctac cttgcgattt cagaaatgat aatgcaagtc ttgccatgtg
     4741 gctcagatgc aagcggcttt tcgatcccct tactcgttga cagccttcgc gacatcgctg
     4801 cgtggtcgct gcaccatatc caccagaccg tattcagccg cctggtcggg gctcatccag
     4861 tagtcccgat ccgtatccgt tgcaattcgc tccacgctct gaccggtgtg atccgccaga
     4921 agccggttca gctgctcccg catgagcaat atttccttgg cctgaatctc gatgtcggcc
     4981 gctacgccgc ggccgccacc cgacggctga tgcagcatca tgcgggagtt cggaagcgca
     5041 tatcgcttgc cttttgctcc ggccgcgagg aggaaggcgc ccatgctggc cgcctgcccg
     5101 atgcacatgg tgctgacgtc gcagcggatg aactgcatcg tgtcgtaaat ggacaatcca
     5161 gcggtgacgg atccccccgg ggagttaatg taaagatgta tgtctttgtc cggattttcc
     5221 gactcgagga acagcagctg cgccaccacg aggttggcca cgttgtcctc cacgggaccg
     5281 accatgaaca cgatgcgttc cttcagaagg cgggaataga tgtcgaacgc ccgctcgcca
     5341 cgtgcagatt gctcaacaac catgggcacc aggcccaggt tagtgatttc gggaatgttg
     5401 gcgtcaccaa tctggctcat tcgattctcc ctgaggtttc cgtcgcctct tcgtcgggct
     5461 ccgcttccgg cgccgcgaag gctcgacccg aaatgatgtc attgtagctg ctctcgacgg
     5521 cctctactgc ggcttgttcc ataacttcgt cgatgacctg ctcctctaag acggccatct
     5581 caatctggcg caactggtct tcgttgccgt agtaccagtt gatgacctgc tgcggctcgg
     5641 cgtactgctc ggcgatgctc tggatccggg cccgcacctt gtcggcgtcg gcttccaggc
     5701 tttttctgga caccagctcg ctgacgatga gaccaacggc aacccgacgt tgcgcctgct
     5761 cggcgaacag ctcgtcggga aggtcaggca gaccgcccgc agcggtaccc ggcatctgaa
     5821 actgctgcag catctgggcc ttgagcgctt cgatttcctg cttgaccacg gccttgggca
     5881 actggaccgt gtgaagctcg ttgagctgat ccattacctg ggttttgacc tggctggcaa
     5941 ccgcgttgtc gagttcgcgc tgcatgttgt tgcgaacgtc ttcccgaaac gcgtccacgc
     6001 ctccttcttc gatgccgaac gccgcaaaaa actcatcgtc cagctcggga agcttgccct
     6061 cggtaacttc cttgacggta acgctgaagg tcgcttgctt gccgcgcagc tcctcggccc
     6121 ggtagtcgtc aggaaacgtt gcctgaaact caccatcggt tcccgcctgc atgccgcgca
     6181 cgccgtgatc gaaatcctcg atcatctgcc ccgcgcccac gatgaaggtc acgtcttctc
     6241 ccgagccgcc ctcgaatgcc tcgtcgtcga tgcgccccgc aaaatcgatc gtgacccggt
     6301 cgccgtcctc agcaccgcga tccacggcca cccactcctt acgctgctcc cgaaggcgct
     6361 ccaccatctc gtccacgtcg agctcggtga tctgggcgtt gggcctgttg atgtgcacgc
     6421 cgctcaaatc cgccagctcg atcttcggaa acacttcgaa agtagcggtg aactcgaagt
     6481 cgatacctgg cgacattttc acgacttcga ggttgggcga cccggccggc gccagttcct
     6541 cctgacggac ggcttccacg aagctcgact gcatcagctc accagccacc tccgccctta
     6601 ccgccggccc gaaacggcgg cgcacctcct tcatgggcac ctttccggct ctgaaacccg
     6661 gcagacgcac ctgctgtgcg gctcggttca gcctttcggt gatctgacct tcgaatgtct
     6721 gcgacggaac cgagatggtc aaccggcgct cgaggccgct catggtctca acggaaatat
     6781 gcatctgtct gccgtgcttg ccgctacctt taactgccgc cctggggttg aaatggggtg
     6841 gacgatgggg ctcgaaccca cgaccgccgg gatcacaacc cggagctcta ccaactgagc
     6901 tacgtccacc gtagcctggt ctttcacccg accgctgggc ggcgccattc taaccgaacc
     6961 atcgagccgc tcgatacgag tctggtacgc ccggaaggac tcgaaccttc aaccctcggc
     7021 ttagaaggcc gatgctctat ccagttgagc tacgggcgca ttctcgatga gaaattggtc
     7081 ggggtagaga gatttgaact cccgacatcc tgctcccaaa gcaggcgcgc taccagactg
     7141 cgctataccc cgaagtggct gttgcacaac ccgcgcccga agcggcgcga tgatactggt
     7201 ggtgttctga gccgtcaaac gcagacgccg ggccctgaac gggtcgatcg gcggtcgggg
     7261 ttggccggtc tgaggccggt tcgttgtaat atgggggcct acaggcaagc ggaatccggg
     7321 tgacgtgaca gcctccatcc tcgacggcaa aagtctggcg aaatcgctca gagcagacct
     7381 ggcggcggcg gtgcaggagc ggctcgaatc cggcgggaga ccaccgggcc ttgcggtcat
     7441 tctggtcgga aacgatccag cctcggaggt ctacgtgcgt cacaagagtc gggactgcga
     7501 ggaagtcggg ttccactccg tggtgcagca tctgtccgag aacgtcaccc agcaggacct
     7561 cgaggaacgc atcgttgccc tcaacgcaaa cccgtccatc gatggcatac tggtccagct
     7621 cccgcttccc gcccacatcg acagtcaccg gatcatcgac cggatcgacc ctctcaagga
     7681 cgttgacggc tttcatccct acaacatcgg ccgtctcgcc ctgcgcagac ccgtgctgcg
     7741 acctggcacg ccaaaaggca tcatgcagct cctggaggtc accggtgtcc cgattcgcgg
     7801 cctggacgca accattctcg gcgcctccaa tcacgtcggc cgaccgatgg gcctggagct
     7861 cctgctggcc ggatgcacgg tgacgaccgc gcacaaattc tcccgcgacg cccgggcctc
     7921 agcccggtcg gccgacatcc tggtgtctgc cgtaggcaag cccgggctcg tcgggggcga
     7981 ctggatcaaa cccggcgcca tcgtgatcga cgtcggcatt acccggggtg aggacggcaa
     8041 gcttcgggga gacgtggtgt tcgacgaggc gatggaagtc gccagctggg tcacgcccgt
     8101 gcccggcggc gtggggccca tgacgcgggt ggccatgctt cagaacaccc tcgccgcagc
     8161 cgacggatcg gactgaaccc ttgcaccgga tctacatctc ggaccttcat ctttcggatg
     8221 aaagcgagcc gcagttcgca acgcttgcgg ctctgctgcg gcacgaggcg cctcgcgccg
     8281 acgccatttt cattcttggc gacctgtgcg aagcgtgggt tggtgacgac gacgactcac
     8341 cgttggctac cgcgcttcgc gcagcgctga agtacgccgg cgagcagacc agtctgcacg
     8401 tcatgcgagg caacagggac ttcctgctcg gcgagcggtt cgcagcggaa atcggatgct
     8461 gcctggtcga ggatccctgt ctgatcgacc ccgacaccct gatcgcccat ggggacgcct
     8521 tctg
//
LOCUS       DBNHKV010000197         4554 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1868580, whole genome shotgun sequence.
ACCESSION   DBNHKV010000197 DBNHKV010000000
VERSION     DBNHKV010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1868580"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /gene="dnaQ"
                     /locus_tag="AC16PP_06295"
     CDS             complement(<1..478)
                     /gene="dnaQ"
                     /locus_tag="AC16PP_06295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HIR6481272.1"
                     /translation="MTRQIVLDTETTGLEAQKGHRVIEIGAVEMIDRKPTGRHFHKYI
                     NPDRDIDDGAFEVHGLSSEFLSDKPRFEDIAQEFLAFVSGAELVIHNAGFDVGFLNYE
                     LAMLEAVPDGIESVCLVTDSLSLARSKHPGQKNNLDALCRRYGVDNSARTLHGALLD"
     gene            complement(475..927)
                     /gene="rnhA"
                     /locus_tag="AC16PP_06300"
     CDS             complement(475..927)
                     /gene="rnhA"
                     /locus_tag="AC16PP_06300"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HIR6481273.1"
                     /translation="MSAPVEIFTDGACRGNPGPGGWAALLRYGDAEKLISGAAPDTTN
                     NRMELQAAISALEVLTRPSSVAITTDSEYLRQGINQWIQKWKRNGWRTSQRKPVKNRD
                     LWQKLDELVQQHQIEWHWVRGHSGHPENEQVDEAANVAIDRMLTGGES"
     gene            complement(911..1693)
                     /locus_tag="AC16PP_06305"
     CDS             complement(911..1693)
                     /locus_tag="AC16PP_06305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6481274.1"
                     /translation="MSAWLATPVGERLLADEAPYVAETARRFHGDTLLWAGCHTPLTD
                     TVRGCMVRNRVFALDGSSPAGEGLTTFRCELDAIPLANNALDGVLLHHALEMAEDPRT
                     ALRELSRVLAAGGRLTVVGFNPFSFWGVRIAYARVFRDSFSGLRLVSPARLVDWLTVL
                     GFELCEEVRFTAYNLPFRLGNTGEQDERSTQQLIPSSWGPRLRNALDHLNPPVGGVYM
                     VSAVKQALGVRRDWRLSAAREPKLAPVAYPKLSARNRIERSR"
     gene            1768..3315
                     /locus_tag="AC16PP_06310"
     CDS             1768..3315
                     /locus_tag="AC16PP_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HIR6481275.1"
                     /translation="MTATAPVVRFASTLRTLMFVILASLVGCQTNPIPEAAETAAAEP
                     PPPVAVPPPVIEPTLTYELKPDLWRDIRERFELAHSLDQKRVQQELRWLRENPRYLHN
                     LKPRMERFLAYIHGEVAARELPGELALLPIVESALDPYAFSHGGAAGLWQFIPATAKR
                     FGLQRNWWYDGRRDPIAATQAALDYLEYLHLLFEDWHVAVAGYNAGEGNVRRAQRKAA
                     PGATFWEMALPRETQMYVPRLLALAAVVTDPEAYGLTLPELSPDVPFVAVDTQGQFDL
                     AKAADTLSMDLDALYDWNPALNQWSTPPDGPHRLMIPALEPALSQAALAAVPAGERVQ
                     WLRIKVADGDTLSEIAHRHRTDTATLRRVNNLTGSSIRAGQAMYIPKSDSAPSRSPRS
                     RAQKGLEYAVQPGDSLWTISRAHGVSMTRIMKANHVGPKDLLKVGQTLTIPGAQRSTP
                     AAGEDGREVIRTVRYGVRKGDSLARIAARFNVRVSEIAQWNQLDVDNYLQPGQSLKLH
                     VNVAAGQ"
     gene            complement(3346..>4554)
                     /locus_tag="AC16PP_06315"
     CDS             complement(3346..>4554)
                     /locus_tag="AC16PP_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6481276.1"
                     /translation="VEYLELSAEGLVNDESISVSEAAIEETYAADSAEALSGDRRQAR
                     HILLEVGASRTAEQAENELNAARERIVGGADFASLAEELSEDPGSAALGGDLGMVAKG
                     IFDPAFEQALWELEVGEISEPVRTDFGYHLIQLDAVDVRELPPLSEQRDDIELRLRRE
                     QAEELFSERLREFDNLAFEQPDGLEGIAAALALETQSVAGVSRSRGDGVFANASLRDA
                     VFASEVLDNGYNTQAVALTDNRAVVARVKARYEPEAIPLDDVRDDIRAAIASEKARVA
                     LREAQQNALARVRSGESVSAVADDYGLGWQTFELVSRSSADAPRAVLSAAFSLPRPGE
                     REKSVGEATLDDGSVAVVTVTRVVDGDVSVMAEREIESMRGFLGNRVASLDFAGLTTT
                     LEREASISTP"
BASE COUNT          815 a         1564 c         1393 g          782 t
ORIGIN      
        1 cgtccagcag cgccccgtgg agcgtgcggg cggagttgtc caccccgtac cggcgacaga
       61 gcgcatccag attgtttttc tggcccggat gcttggaccg ggcgagggaa agcgagtccg
      121 ttacgaggca aacgctctcg attccgtccg gaaccgcttc gagcattgcc agctcgtagt
      181 tcagaaatcc gacgtcgaaa cccgcattgt ggatgacgag ctccgcaccc gaaacaaaag
      241 cgagaaactc ctgcgcgatg tcctcgaacc ggggcttatc gctgagaaac tccgagctca
      301 gaccgtgcac ctcgaaggcg ccgtcatcaa tatcgcgatc ggggttgatg tacttgtgga
      361 agtgacggcc ggtcggcttg cggtcgatca tttccacggc accgatctcg atgacccgat
      421 gccccttctg agcttcgagg ccggtggtct cagtgtcgag aacgatctgc cgcgtcaaga
      481 ttcgcctcct gtgagcatgc ggtcgatggc cacgttcgcc gcttcgtcca cctgttcgtt
      541 ttccggatgc ccgctgtgcc cgcgtaccca atgccactcg atctggtgct gctgcaccag
      601 ctcatccagc ttttgccaga gatcgcggtt cttcaccggc tttcgttgag acgttcgcca
      661 gccgttgcgc ttccatttct gaatccactg gttgatgccc tgcctgagat attcagagtc
      721 ggtggtaatg gccacgctgc tcggccgcgt gagcacctcg agcgcggaga tcgccgcctg
      781 cagctccatg cggttgttgg tcgtgtccgg agcagcaccg ctgatgagct tctcggcatc
      841 cccataccgc agcagagcgg cccagccgcc aggccccgga ttgcccctgc aggccccgtc
      901 ggtgaagatc tcaacgggag cgctcaatcc ggttccgcgc gctcagtttc ggatacgcca
      961 caggggcgag tttcggttcc cgggcagcag acagacgcca gtcccggcga acgccgagcg
     1021 cctgcttgac cgcgctgacc atgtaaaccc cgccgaccgg ggggttgagg tgatccaacg
     1081 cgtttcgcag ccggggaccc caactcgacg gaatcagctg ctgcgtcgac cgttcgtcct
     1141 gctcgccggt atttcccaat ctgaacggca ggttgtaggc ggtaaagcgc acttcctcgc
     1201 aaagctcgaa accgagcacg gtcagccagt ccacaagccg agcggggctg accagccgca
     1261 gcccgctgaa gctgtcgcga aagactcgcg cgtaagcgat ccgaacgccc cagaaactga
     1321 acgggttgaa ccccacaacc gtcagccgcc cgccggccgc aagcacgcgg gagagctccc
     1381 gcagcgcggt gcgcgggtcc tcggccatct ccagcgcatg gtgaagcagc acaccatcca
     1441 gggcgttgtt ggccagaggt atggcgtcca gctcgcagcg aaaggtcgtg aggccctcgc
     1501 ccgccgggct cgaaccatcg agcgcgaaca cgcgattgcg aaccatgcat ccgcgcacgg
     1561 tatcagtcag gggggtatgg caccctgccc agagcagcgt atcgccgtga aagcggcggg
     1621 ccgtttccgc cacgtagggc gcctcgtcgg caaggaggcg ctcaccgacc ggtgtagcca
     1681 gccaggcgga catctgatga cgcaaggatc ttgccaatcg cacagggata ccgcaaagtt
     1741 gaaggatcat tctgaaggca ccactcgatg accgcaaccg cacctgtcgt tcggtttgcc
     1801 tcgaccctgc gcacgctgat gtttgtgatc ctggcgtcgc tcgtggggtg ccagaccaat
     1861 cccatccccg aagcggccga aaccgcggcc gccgagcctc ccccgcccgt cgcggttccg
     1921 cctcccgtca tcgagccgac gctcacctac gagctgaaac ccgatctgtg gcgcgacatc
     1981 cgcgagcgct ttgagctggc ccacagcctc gaccagaaac gcgtgcagca ggagctgcgc
     2041 tggctgcgcg agaatccgcg ctacctgcac aatctgaaac cccgaatgga gcggttcctc
     2101 gcctacattc acggggaggt ggccgctcgg gagcttcccg gtgagctcgc cctgctcccc
     2161 atcgtggaga gcgcgctcga cccgtatgcg ttttctcacg gcggcgccgc cggtctgtgg
     2221 cagttcatcc ccgccacggc gaagcgattc gggctgcagc gaaactggtg gtacgacggc
     2281 cgtcgggatc ccatcgcggc tactcaagcc gccctcgact acctcgaata tcttcatctg
     2341 cttttcgagg actggcatgt tgccgtcgcg ggctacaacg ctggagaggg caacgtccgc
     2401 cgcgctcagc ggaaagccgc accgggggcc accttctggg agatggctct gccccgggaa
     2461 acccagatgt acgtgccgcg cctgctggcg ttggcagcgg tggtaaccga ccccgaggct
     2521 tacggcctga cgctgcccga gctttcgccg gacgtgccgt tcgtggccgt cgacacgcag
     2581 ggacagttcg acctcgccaa ggcagccgac accctttcca tggatctcga cgcgctctac
     2641 gactggaatc cggccctgaa ccagtggtcg acgccgccgg atggtcctca ccggctgatg
     2701 attcccgcac ttgaaccagc actgagccag gcggcccttg cggccgttcc cgctggcgag
     2761 cgggtccagt ggctgcggat caaggtggca gacggagaca ccctcagcga aatcgcccac
     2821 cgtcaccgaa cggacacggc caccttgcga cgggtgaaca atctgacggg ctcctcaatt
     2881 cgtgccggcc aggctatgta cattcccaaa tcggattcgg cccccagccg cagtccccgc
     2941 agccgcgctc agaaaggact ggaatacgcg gtgcagcccg gagattccct gtggaccatc
     3001 agtcgggcgc acggggtttc gatgacgcgc atcatgaaag ccaatcacgt ggggcccaag
     3061 gacctgctga aggtaggcca gactctcacc attcccggcg ctcagcgctc gacgcccgct
     3121 gcgggagaag acggacggga agtaattcgc acggtgcgct acggcgtgcg caagggcgac
     3181 tcgctggcgc gcatcgccgc tcgcttcaac gtgcgggtca gcgagatcgc gcagtggaac
     3241 cagctggacg tggacaacta cctgcagccc ggccagtcgc tgaaactgca cgtcaacgta
     3301 gcggctggac agtaactgac cagccggccg ccggacgggg tcgacctacg gcgtgctgat
     3361 cgacgcttcc ctttccagag tggtcgtcag ccccgcgaaa tccagagaag ccacgcgatt
     3421 tcccaggaag ccgcgcatgc tttcgatctc tctttccgcc atcacggaaa catccccgtc
     3481 gaccacacgg gtaacggtga ccaccgcaac ggagccgtca tcgagcgtgg cttcacccac
     3541 gctcttctcc cgctcaccgg gccgtggcag gctgaaggcc gccgagagaa cggctcgcgg
     3601 ggcgtcggcc gagctgcggc tgacgagttc gaaggtctgc caacccaggc cgtagtcgtc
     3661 ggccaccgcg gaaacgctct cgccggaacg aacgcgcgcc agcgcgttct gctgagcttc
     3721 tctgagcgca acccgcgcct tttccgacgc gatggccgcg cgtatgtcat cgcgaacgtc
     3781 atccagggga atcgcctccg gctcgtatcg ggccttgacg cgggcgacca ccgcgcggtt
     3841 gtccgtcagc gctaccgcct gtgtgttgta gccgttgtcc agaacctcgc tggcgaatac
     3901 cgcatccctc aaggacgcgt tggcgaaaac accgtcgccg cgcgaacgtg acacgccggc
     3961 caccgactga gtctccagcg ccagggccgc ggcgataccc tcgagaccgt cgggttgctc
     4021 gaaagccaga ttgtcgaact cgcgcagacg ctcggagaag agctcttcgg cctgttcccg
     4081 ccgcagccgc agctcgatgt catcgcgttg ctcggacagc ggcggcagct cccgcacgtc
     4141 gacggcgtcc agctggatca ggtggtagcc aaagtcggtt ctgaccggct cggaaatctc
     4201 gccgacctcg agctcccaga gcgcttgctc gaacgccgga tcgaaaatgc ccttggccac
     4261 catcccgaga tcgccgccca gcgcggccga tccaggatcc tcgctgagct cctcggccag
     4321 ggacgcgaag tccgcgccgc ccacgattcg ctctcgcgcc gcgttgagct cgttctcggc
     4381 ctgctccgcc gtccggctgg cgccaacttc cagcaggatg tggcgcgcct gtcggcgatc
     4441 gccggaaaga gcctccgccg aatccgccgc ataggtttcc tcgatggcgg cctcgctcac
     4501 cgagatggac tcgtcgttca ccagcccttc tgcactgagc tcgagatact cgac
//
LOCUS       DBNHKV010000198         2887 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1797684, whole genome shotgun sequence.
ACCESSION   DBNHKV010000198 DBNHKV010000000
VERSION     DBNHKV010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1797684"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..929)
                     /locus_tag="AC16PP_06320"
     CDS             complement(42..929)
                     /locus_tag="AC16PP_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6481277.1"
                     /translation="MSRILEELVADARELARRALQLVPAAPSVDWENTLAASWRETSW
                     GSGFAAHSELDDIELDDLLCVDDQKRSLDLNTQQFLAGAPANNVLLWGARGTGKSSLI
                     HALLNHYHARGLRLVEVSKTRLSGLPEIVLRLAAEPYRFVLFCDDLSFEADDASYKEL
                     KSALEGSVFKASSNVLIYATSNRRHLLPEYMSDNAQSGFVEGELHQSEAVEEKISLSD
                     RFGLWLSFYPFKQDAYLDVVRHWLGRLASEHDAEFVWDDEVRAEALRWALARGVRSGR
                     TAQHFARHWVGRLIINSKQ"
     gene            1098..1244
                     /locus_tag="AC16PP_06325"
     CDS             1098..1244
                     /locus_tag="AC16PP_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481278.1"
                     /translation="MGERTGEDQRKGPKDRRTAAQDRRNAERMAEDLNPRRDPERRGR
                     RTGD"
     gene            complement(1265..1669)
                     /locus_tag="AC16PP_06330"
     CDS             complement(1265..1669)
                     /locus_tag="AC16PP_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481279.1"
                     /translation="MGSLLVVIAIACTTVLWVKGARKNRQRWLARLDLPGTWRWRPSD
                     SAESEVQGSLELSGGLSSGAYRLVEADVEEAGNWALEGHTLLLSPTGTASTGHYEIRF
                     FDEGKIGIDGPGRERRIYVKERTNVVPLRRRG"
     gene            complement(1673..2698)
                     /locus_tag="AC16PP_06335"
     CDS             complement(1673..2698)
                     /locus_tag="AC16PP_06335"
                     /inference="COORDINATES: protein motif:HMM:NF022723.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3080 family protein"
                     /protein_id="HIR6481280.1"
                     /translation="MWTSAVAGWCRRFALAGGVLVGVQCSGPPDNAFADYLSRVQRLL
                     ETDVSMDAEGSLPRYPNRGLLRRDVASLSIDVLDFIELHDCDLAGVVGFRNSPLGRLQ
                     TPSQRLGYESEWLAGARSCASDGVGWLEPLIAKKEAALPAVFWNATFAGEEFARLLGR
                     FEASETPGAGYLLRELGDAYATLATDRFDLGGFERVLGALGSIPWLGQARRAWDLERR
                     HLDAVAGALTEGLEGVCRNGAPTPKSRALVNVFQRYYVDALQPGIARRHGTDRDAVAA
                     VAALVGLFGGDVPGEFAVWYGQVLAPEPRRSEWSRLASVRLEHAAAWNRLFARCGVDP
                     ARGLAQH"
     gene            complement(2667..>2887)
                     /locus_tag="AC16PP_06340"
     CDS             complement(2667..>2887)
                     /locus_tag="AC16PP_06340"
                     /inference="COORDINATES: protein motif:HMM:NF016351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF503 family protein"
                     /protein_id="HIR6481281.1"
                     /translation="QRLKGIRDRFGRSSNVAVCESAYQDSLQRAEWSFVASASSRAVV
                     EKSLAEIERSLSLSVDAELVDVRRRWLV"
BASE COUNT          560 a          997 c          872 g          458 t
ORIGIN      
        1 gcccggatct ttcgatcttc tagaaggttc acacgattcc cttattgctt gctgttgatg
       61 atgagccggc cgacccagtg gcgcgcaaaa tgctgcgctg ttcggccact tctcactccg
      121 cgagcgagag cccagcgcag ggcttctgct ctgacctcgt cgtcccacac gaattcggcg
      181 tcgtgctcac ttgcgagcct gccgagccag tgcctgacga catcgagata ggcgtcctgt
      241 ttgaaaggat aaaaggacaa ccacaacccg aagcgatcgg atagagagat tttttcttcg
      301 accgcctcgg actgatgcag ttcgccttcc acgaaaccgc tctgggcgtt gtccgacatg
      361 tactcgggaa gcaggtgccg gcggttggag gtggcgtaga tcaggacgtt gctcgacgcc
      421 ttgaaaaccg atccttccag agcgctcttg agttccttgt agctggcatc atccgcctcg
      481 aaggagaggt cgtcacagaa cagcacgaag cggtagggct ctgcagccag tcgcaggacg
      541 atctccggca ggccggacag gcgcgtcttg ctgacttcga cgagcctcaa tccccgtgcg
      601 tgatagtgat tcaggagcgc atggatgagc gacgacttac cggtgccgcg ggcaccccac
      661 agcagcacgt tgttggccgg ggcaccggcg agaaactgtt gcgtgttgag atccagcgac
      721 cgcttctgat cgtccacgca gagaagatcg tccagctcaa tgtcgtccag ctcggaatgt
      781 gcagcaaatc ccgagcccca tgacgtctcc cgccagctgg cggccagggt gttttcccaa
      841 tccacggacg gcgccgccgg gacgagctgc agcgcccggc gagccagctc gcgggcgtcg
      901 gcgacgagtt cctccaggat tctgctcacg aaaagtccag ggtttcaagc gggcacaatt
      961 gtaaccagaa accgaccgga aactgaccac gtgcggtgag gcatgtatct atccatgtta
     1021 gacttcttcg gggtcgcgcc cggaatgaac gggcccattc caagccagca caaccagagc
     1081 catcgaactg cgcaaacatg ggcgaacgca ccggagaaga tcagcgtaag ggaccaaagg
     1141 atcgacgcac ggcggcccag gaccgtcgca acgccgagcg catggccgag gacctcaatc
     1201 cgcggcgcga cccggagcgg cggggccgcc ggacgggcga ctagacgaac gggcttgcct
     1261 tgcttcagcc gcgcctgcgg agcggcacca cgttggtccg ctccttgacg tagattcgcc
     1321 gctcccggcc gggcccatcg attccgatct tgccctcgtc gaaaaagcgg atttcgtagt
     1381 gccccgtgct cgcggtcccg gtcgggctca aaagcagggt atgtccctct aaagcccagt
     1441 tcccggcctc ttcgacatcg gcttccacca gccgataagc tccagagctc aggccaccgc
     1501 tcagctcgag gctgccctga acctccgatt cggcggaatc agacggccgc cagcgccacg
     1561 taccgggcag atccaggcgc gcaagccagc gctgccggtt cttccgggca cctttcaccc
     1621 agagcacggt agtgcaggcg atggcaatga cgaccagcag agaacccatg gctcaatgct
     1681 gcgccaagcc ccgcgccgga tcaactccac agcgcgcaaa gagcctgttc caggcagcgg
     1741 cgtgctcaag gcgcaccgac gcgagtcggg accattcact ccgccgaggc tcgggggcga
     1801 gcacctgtcc gtaccagacg gcgaattcgc cgggcacgtc gccaccgaag agcccgacga
     1861 gcgcggcaac tgcagcaacg gcatcccggt cggtcccatg ccgccgcgcg atgcccggct
     1921 gcagggcgtc gacgtagtaa cgctggaaga cgtttacgag tgcccgactc ttcggcgtcg
     1981 gggcgccgtt tcgacacacc ccttcgagcc cctcggtcag cgccccggcc accgcgtcca
     2041 gatgacgcct ctccagatcc cacgcccgtc tggcctggcc aagccagggg attgagccga
     2101 gcgctccgag cacccgctcg aacccgccga ggtcgaaccg gtcggtggcg agcgtcgcgt
     2161 aggcgtcccc cagttcccgc agcaggtagc ccgcccccgg cgtctcggac gcctcgaacc
     2221 tacccagcag ccgggcgaac tcctcccccg caaacgtggc gttccagaag acggccggaa
     2281 gcgcagcctc cttcttcgcg atcagcggct ccagccaccc caccccatcg gacgcgcagg
     2341 accgagcgcc cgcaagccac tcagactcgt agcccaggcg ctggctcggc gtctgcaaac
     2401 gccccagcgg gctgttgcga aaccccacca ccccggccag gtcgcagtcg tgcagctcga
     2461 taaagtccag aacgtcgatg ctcagtgacg ccacgtcgcg gcgcaggagg ccccgattcg
     2521 gataacgagg caaggagcct tcagcgtcca tggaaacgtc ggtttcgagc aggcgctgaa
     2581 cgcgagacag gtagtcggca aaggcgttat ccggcgggcc gctgcactga acgccgacca
     2641 gcacaccccc ggccagcgcg aagcgcctac accagccagc gacggcggac gtccacaagc
     2701 tctgcatcga cgctgagact caggctgcgt tcgatctcgg caaggctctt ctcgaccacg
     2761 gctcggctgc tcgccgacgc cacgaacgac cactctgctc gctgcaggga gtcctggtaa
     2821 gcgctttcgc atacggccac gttcgagctt cgcccgaacc ggtcccggat ccccttgaga
     2881 cgctgac
//
LOCUS       DBNHKV010000199         3911 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_168603, whole genome shotgun sequence.
ACCESSION   DBNHKV010000199 DBNHKV010000000
VERSION     DBNHKV010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_168603"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="AC16PP_06345"
     CDS             <1..378
                     /locus_tag="AC16PP_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481282.1"
                     /translation="GGGTDRIDRDLLSDLLGDEAADDLEQLQQMLKALEEAGYIRPTD
                     DDGWEITPRGSRMIGQKALGEIYARLKRQNLGNHAVPEEGRFGERLEQTKAYEFGDPF
                     HLHMPRTLRNAIDREGPQTPVSL"
     gene            433..1002
                     /locus_tag="AC16PP_06350"
     CDS             433..1002
                     /locus_tag="AC16PP_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024909.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR6481283.1"
                     /translation="MLVDLSWSMALRGSFQAAKKVALALHNLITSQYPKDTFYIIGFA
                     AYAKELKPKDLPYLQWDEYVLGTNMQHALLLAERLLAKHQAGSKQIIMISDGEPTAHL
                     ENGQAQFAYPPTPETIRATFRAVKHCTARGVAINTFMLDSSYYLKAFMDEIAKINGGR
                     VFYTTPEQLGEYILVDYVQNKKKRLARRA"
     gene            complement(1003..2169)
                     /locus_tag="AC16PP_06355"
     CDS             complement(1003..2169)
                     /locus_tag="AC16PP_06355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017134109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6481284.1"
                     /translation="MDFEITEEQQLIKDAVGRICARFPDEYWARCDSEHEFPWDFYHA
                     MAEAGWIGVAIPEAYGGSGRGITEASLVLETVSASGAAMNGASGLHLSIFGMHPVVKY
                     GSEEMKQAYLPRVASGELHVAFGVTEPNAGTDTTSIETFARREGDHYVVRGRKVWTTK
                     ALDSERVLLLVRTTPADEVARRTDGLTLLLAELQRPEVTISAIDKAGRNAVASCEVVY
                     DDLPVAVTDRVGEEGKGFRYLLDGLNAERILVASEALGIGRAAMRRAVDYANDRVVFN
                     RPIGQNQGVAFPLGEAQMRLDAAELMIRKASWLLDKGSPCGAEANMAKWLAADAAYQA
                     ADQAMQTHGGFGYAKESHVERYWREARLMRIAPISQEMILNYVTEHVLGLPRSY"
     gene            2289..3095
                     /locus_tag="AC16PP_06360"
     CDS             2289..3095
                     /locus_tag="AC16PP_06360"
                     /inference="COORDINATES: protein motif:HMM:NF004781.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR6481285.1"
                     /translation="MDEIELKTTRMRAGIEDDGIGWVIFNHPERHNALSLEMWQGLGD
                     ILEAFAGDDRVNVAIMRGAGGKAFISGADISEFDTSRANSTQKADYNRIMGRANHWLA
                     EFDKPLIALIEGYCIGGGLATALAADIRIATPDSRFGIPAARLGLGYDYEGLAKLVRI
                     VGPARARDIMFSARFFDASEAHGMGLINFVHTRDGIEEETLTYARRIAGNAPMTVRAA
                     KAAVNAWERGERSDELAEVRKLVDACFDSEDYKEGRRAFKEKRTPDFAGR"
     gene            3350..>3911
                     /locus_tag="AC16PP_06365"
     CDS             3350..>3911
                     /locus_tag="AC16PP_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HIR6481286.1"
                     /translation="MNTLRVREFVKFAAIFLLTTAALGGCASDGAPPPASNTTIPDFA
                     EDYRIGVGDTLSVSVWRNDDLSITAPVRPDGKISVPLAGDVLVGDKTPEEVSAEITEK
                     IGAYIRDPFVTVAVTNMGSTEYRSRVRVTGAVDRPVSLPYRQGMTVLDVVLEAGGINE
                     FANGSKSTLFRRSGERFEINLDRLLKR"
BASE COUNT          743 a         1289 c         1220 g          659 t
ORIGIN      
        1 ggtgggggca cggaccgtat cgatcgggac ctgttgagcg acctgctcgg ggacgaagcg
       61 gccgacgacc tcgagcagct gcagcagatg ctcaaggccc tggaggaagc gggctacatc
      121 cgccccaccg acgacgacgg ctgggaaatc acgccgaggg gttcccgcat gatcggccag
      181 aaggcccttg gcgagattta cgccaggttg aagcggcaga acctcggcaa tcatgccgtg
      241 cccgaggagg ggcgtttcgg cgagcggctg gagcagacca aagcctacga gttcggcgac
      301 ccgttccatc tgcacatgcc ccgaaccctg cgcaacgcca tcgatcggga gggcccgcag
      361 acgccggtga gcctctagcc cgaggacttc gagatctacc gcatcgagct catctcgtcc
      421 acggccacgg ccatgctggt ggacctgtcc tggtccatgg cgctcagggg ctctttccag
      481 gcggcaaaaa aagtggcgct ggcgctgcac aacctcatca cctcgcaata tcccaaggac
      541 accttctaca tcatcggctt tgccgcttac gccaaggagc tgaagccgaa ggatctaccc
      601 taccttcagt gggacgagta cgtgctgggg accaacatgc agcacgccct gctgctcgcg
      661 gagcgtctcc tggccaagca ccaggcaggc agcaagcaga tcatcatgat ttctgacggg
      721 gaacccaccg cccatctgga gaacggccag gcccagttcg cctacccgcc cacacccgag
      781 accattcgcg ccacctttcg cgccgtgaag cactgcaccg cgcggggcgt ggcgatcaac
      841 acgttcatgc tggattcgag ctactacctc aaagccttca tggacgagat cgccaagatc
      901 aacggcggcc gcgttttcta cacgacgccc gagcagctcg gcgagtacat cctcgtcgac
      961 tacgtgcaga acaagaagaa gcggctcgcg cgcagggcct gatcagtaag agcgcggcag
     1021 cccgagcacg tgctcggtga cgtagttcag gatcatctcc tgggagatcg gcgcgatccg
     1081 catgagcctc gcttcccgcc agtagcgctc cacgtgggac tccttcgcgt aaccgaaccc
     1141 gccgtgggtc tgcatggcct gatccgccgc ctggtaagcc gcgtcggcgg ccagccactt
     1201 ggccatgttg gcctccgccc cgcagggcga acctttgtcc agcagccagg acgccttgcg
     1261 gatcataagc tccgccgcgt ccaggcgcat ctgcgcttcg cccagaggaa aggccacgcc
     1321 ctggttctgg ccgatcggcc gattgaagac cacgcgatcg ttggcgtagt ccaccgcccg
     1381 acgcatggcc gcgcggccga tgccgagcgc ttccgaggcc accagaatac gctcggcgtt
     1441 gaggccatcg agcaggtaac ggaaaccctt gccctcctcg ccgacgcggt cggtgaccgc
     1501 cacaggcagg tcgtcgtaga ccacctcgca gctggccacc gcgttgcgcc ccgccttgtc
     1561 gatggctgat atggtgacct cgggtctctg aagctccgcg agcaggagcg taagtccatc
     1621 ggtgcgacgg gccacctcgt ctgccggcgt ggtgcgaacg agcagaagga cgcgttccga
     1681 atccagcgcc ttggtcgtcc agaccttgcg cccccgcacc acgtagtggt cgccctcgcg
     1741 ccgggcaaac gtctcgatgg acgtggtgtc cgttcccgcg ttgggctcgg tcaccccgaa
     1801 agccacgtgc agctcgccgc tcgccacccg aggcagatag gcctgcttca tctcctcgct
     1861 gccgtacttg accacgggat gcatgccgaa gatggaaagg tgcagcccgg aggccccgtt
     1921 catggccgcg cccgaggcgg agacggtttc cagcaccagg ctcgcttccg tgatgccgcg
     1981 cccgctgccg ccgtaagcct cggggatggc caccccgatc cagcccgctt ccgccatggc
     2041 gtggtaaaag tcccacggaa actcatgctc gctgtcgcag cgggcccagt actcgtcagg
     2101 aaaacgggcg cagatcctgc cgaccgcatc cttgatgagc tgctgctctt cggtgatctc
     2161 gaaatccacg ggtctcatcc actgctgacg cagagctgat gtttgggcag aagccgctcg
     2221 agtgcaaggc gcagttggca aagatcatcc gggcgggtaa cgtttcgccg cgagctgagg
     2281 agacttcgat ggacgagatc gagcttaaga ccacacgcat gcgggcgggc atcgaagacg
     2341 acggcatcgg ctgggtgata ttcaaccacc ccgaacgcca caacgcgctc tcgctggaga
     2401 tgtggcaggg tctgggcgac attctggaag ccttcgccgg cgacgatcgc gtcaacgtcg
     2461 ccatcatgcg cggcgcgggc ggcaaagcct tcatctcggg cgccgacatc tcagagttcg
     2521 acacgagtcg ggccaactcg acccagaagg ccgactacaa tcggatcatg ggccgcgcca
     2581 accactggct ggctgaattc gacaagccct tgatcgcgct gatcgagggt tactgcatcg
     2641 gcggcggtct ggctaccgcg ctcgccgcag acatccgcat cgcaaccccc gactcacgct
     2701 ttggcatccc ggcagcccgc ctgggtctgg gttacgacta cgaggggctg gccaagctgg
     2761 tacggatcgt cggtcccgcc cgcgcgcggg acatcatgtt ctccgcgcgc ttcttcgacg
     2821 cctccgaagc ccacggcatg ggcctgatca acttcgttca cacccgcgac gggatcgagg
     2881 aagaaaccct gacctacgcg cggcgcattg ccggcaacgc gccgatgacg gtgagggcgg
     2941 ccaaagcggc ggtgaacgcc tgggaacgcg gcgagcgctc cgacgagctg gcagaagtca
     3001 ggaaactggt ggacgcctgc ttcgatagcg aggactacaa ggaaggacgg cgtgccttca
     3061 aggaaaagag aaccccagac tttgcaggtc gatgactagt cgatctgctc ccttgcaaga
     3121 cacgccgtcc tttaccggag atccgtccgc gcgctgcggg ctcggatcgc atctgtagta
     3181 ggacgaattc tgtctggggc gtttcgtcga ggatcattgt tgacgaacta cccatcgacg
     3241 tctgttgcgc tcatgtgagc ctatgtaatg ggctggtaac ggcacgcccc aacgacccca
     3301 ttaaggggta tgatcccacc gtctgtaacc ccgactcagg tgacccagaa tgaatacgct
     3361 ccgcgttcgc gaattcgtca aatttgcagc aatcttcctc ctgacaacgg ccgccctcgg
     3421 cggctgcgca tccgacggcg cgccgccgcc cgcctcgaac acgacgatcc cggactttgc
     3481 cgaggactac cggatcggcg tgggcgacac gctttcggtg agcgtctggc gcaacgacga
     3541 tctcagcatc acggcacccg tgcggccgga cggaaagatt tcggtaccgc tggcgggcga
     3601 tgtgctcgtg ggcgacaaga cgccggagga ggtcagcgcg gagattaccg agaagatcgg
     3661 cgcgtacatt cgcgaccctt tcgtcaccgt cgccgttacg aacatgggca gcacggagta
     3721 tcgctcccgg gtgagggtga caggagcggt ggaccggccc gtgtcgctgc cttaccgcca
     3781 gggcatgacg gttctcgacg tggtgctcga ggccggtggg attaacgagt ttgccaacgg
     3841 atcgaaatcg accctgttcc ggcgcagcgg cgagcgcttc gagatcaatc tggaccggct
     3901 gctcaagcgc g
//
LOCUS       DBNHKV010000200         5657 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1728912, whole genome shotgun sequence.
ACCESSION   DBNHKV010000200 DBNHKV010000000
VERSION     DBNHKV010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1728912"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="AC16PP_06370"
     CDS             <1..351
                     /locus_tag="AC16PP_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481287.1"
                     /translation="PEKLSSPELFDQASDLLDDAMALTPRGPRLDARIREVTALLAAY
                     RDPIPVVFVSDNRTQVTLSTIGRLGSFERKQLDLRPGSYTVIGSRDGCRDVRADIVVK
                     PNMQPVDIRCTERL"
     gene            359..2449
                     /locus_tag="AC16PP_06375"
     CDS             359..2449
                     /locus_tag="AC16PP_06375"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR6481288.1"
                     /translation="MSTRNGQDNNASQVQADDLEIIEAVSYTPREQAPKRKLPLTPFQ
                     IGAVLVAIPVMAIMWFLFTAKSVRLEFDPPAASESVSEGPAIALGDIYLMLDGEYRVS
                     ARAEGYEPLDELLVVGDARSQTHRFEFTKLPGRVTFLSNPDGAEVIVAGALLGRTPLD
                     GVKVPAGPATISMKLDRYQRLDMEAEIEGLDRAQTVEGSLLPDWAEVSVSSIPAGAEI
                     LLNDQPTGRLTPAVIEVPTGEHEIRLKAPGHRGHRQRILVAALDEVTLPEVTLLRADG
                     LLQVTSRPAGAGVTLNGQFQGETPVEIAVKSGISYRVKIFKAGFAPGERSVRVASGGE
                     RTVDLTLTRLTGQLIVTTQPAEAELFVDGRNLGNANQTLTLPTVTHRVEIALDGYAGY
                     STEITPRNGVTQEIKVRLLTLEEARLAAMKPVIQTSQGQELVLLNPGPFTMGASRREP
                     GRRANETLREVGMERIFYLSRTEVTNAQFLDFAPGHDSGEFQDHELNKDDQPVVGVSW
                     QEAALYCNWLSAREGLPPFYATDLGKVVATNPAATGYRLPTEAEWEWAARQNGPDSTG
                     YRFPWGTALPPPDRHGNYADRSAAHLVGRVVFGYNDNHIVSAETGTFAPNAIGIYDLS
                     GNVAEWVNDFYEIPGAEPTKDPLGPDTGEYRVIRGSSWMNGTITDLRLSFRDYGVDGR
                     EDVGFRIARFAEPS"
     gene            2446..2652
                     /locus_tag="AC16PP_06380"
     CDS             2446..2652
                     /locus_tag="AC16PP_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481289.1"
                     /translation="MKGPLLFAAAALLASTMGTGFAVAAEEAADTRDRAGEEEEADGE
                     PSPEIFVPSEEISEDFAVSFPVDI"
     gene            2674..3474
                     /locus_tag="AC16PP_06385"
     CDS             2674..3474
                     /locus_tag="AC16PP_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6481290.1"
                     /translation="MKRQLPFELIYQSSALVLAVILVHLVYVTVIRPNADAIIAAQAE
                     RAAAGEAYAQERSLYVVLKDYEQESCFVLMLWAMAIMGYKTRSALRERSLLQEDIVKI
                     SEGMSVLPEDTREYARAVQALPDENRNYLLPRALMAALQRFASTRNVQDVSEAVRSVC
                     ESESDRLDSELSMVRYIAWAIPSIGFIGTVRGISEALGQAYEAVEGNIAGVTTSLGVA
                     FNSTFTALVISIVIMFLMHQLQLIQERLVLDAQTYCDERLLRHLQVRD"
     gene            3474..4724
                     /locus_tag="AC16PP_06390"
     CDS             3474..4724
                     /locus_tag="AC16PP_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481291.1"
                     /translation="MDYVLELNDSDLSLHSGETLAYHAPGVALVRENEILFGDPALRL
                     SRAHPRQINQHYLARMNADPLPYPVSNAANHADLVYLHLKQLQDLMRRDPGDLVLAVP
                     AILSADQLGVLLGITQECGMAVSGFVDSAVAAASVSELPASVCHLDAHLHRTCLTLLD
                     VGADVVRTGADEVADCGVANLLEGWVNVIADRFVRDTRFDPLHAAETEQQLYNQVYDW
                     AMGQSSSDSEIGIEIGYRDQVRRVEVSRSLLEEKARQRFAHLSERLPEDVHLALSART
                     ARLPGLTAYLGAQGWEYSVLDGSALAAGCSANDSSIRAEDGRLRLVSRLPLRHGRGPQ
                     SIVAPGPDESGRPTHVLCDACAVPIGADDLPLQLKESEAGVYLDADETVRLNGEAITT
                     SRRLRAGDELTAGGKSYLLIRVVG"
     gene            4726..>5657
                     /locus_tag="AC16PP_06395"
     CDS             4726..>5657
                     /locus_tag="AC16PP_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481292.1"
                     /translation="MARNRRFSVFSLSFLDVMSCGFGAVVLIFLIINHAAEEDAKVVN
                     EELLSEIRLLDYQVQNGERDLFELKEQLDAILQRLSDSSRKLVSTETSLKETSKEFAD
                     LAAESLAQEASLEELKSDVDTREEELARLKSINEANEGGRIRSFTGEGDRQYLTGLKV
                     GGKHILIAVDTSTSMLDESIVNVIRRRNMPDDRKRMSPKWQRAVRTVEWLTAQLPLDA
                     SFQIYGFNVEARSLLEEHHGQWIALAEGRELDDALEALREVIPDRGTSVANLISAVNE
                     LNPPPDNVYLIIDSLPTQGTREPRRATISGGDRL"
BASE COUNT         1048 a         1842 c         1812 g          955 t
ORIGIN      
        1 ccggagaagc tctcgtcgcc ggaactattc gatcaggcga gcgacctgct cgatgacgcc
       61 atggccctga cgccccgggg accacggctc gacgctcgga tcagggaagt caccgccctg
      121 ctcgccgcgt atcgggaccc gattcccgtc gtcttcgtct cggacaaccg aacccaggtc
      181 accctgtcca ccatcggcag gctgggcagc ttcgagcgca agcaactcga cctgcggcca
      241 ggctcctaca cggtcatcgg cagccgtgac ggctgccgcg atgtcagggc cgacatcgtc
      301 gtcaaaccga acatgcagcc cgttgacatc cgctgcaccg agcggctcta ggcttgtcgt
      361 gagcactcga aacggccagg acaacaacgc ctcccaggtt caggccgacg atctggagat
      421 catcgaggcg gtaagctaca cgcctcggga gcaggcgccg aaacgaaagc tgccgctgac
      481 gccgtttcag atcggcgccg tgctcgtagc gattcccgtg atggcgatca tgtggtttct
      541 cttcaccgcg aaatccgtgc ggctggagtt cgaccccccg gccgcctcag agagcgtgag
      601 cgaaggtccg gccattgccc tgggcgacat ttatctgatg ctcgacggcg agtatcgggt
      661 aagtgctcgc gccgagggct acgagccact cgacgagctc ctcgtcgtcg gcgacgcgcg
      721 cagccagaca catcgcttcg agttcacgaa gcttcccggt cgggtcacct ttctgagcaa
      781 tcccgacggc gcggaggtca tcgtggccgg ggcgctgctc ggacgaacgc cactggacgg
      841 tgtcaaggtc cctgccggcc cggcgacgat atccatgaag ctggaccgct accagcgtct
      901 ggatatggag gccgagatcg aaggtctgga tcgagctcag accgtggagg gttccctgct
      961 tcccgactgg gccgaggtga gcgtttccag catcccggcc ggagcggaga tcctcctgaa
     1021 cgaccaaccc acgggtcgtc ttaccccagc cgtcatcgag gtgcccaccg gcgagcacga
     1081 gataaggctc aaggcgcccg gccaccgcgg gcatcggcaa cgcatcctgg tcgccgcgct
     1141 ggatgaggtg acgctgccgg aagtcaccct gctccgcgcc gacgggctgc tgcaggtcac
     1201 gagccgaccg gctggagcgg gcgtcacgct gaacgggcag tttcagggcg agacgcctgt
     1261 ggagatcgcg gtgaaatccg gtatcagcta ccgggtgaag atcttcaagg caggtttcgc
     1321 ccccggcgag cgcagcgttc gggtggcctc cggcggtgag cgcacggtag acctgacgct
     1381 cacgcgcctt acaggtcagc tcatcgtcac gacccagccc gccgaagcag agctcttcgt
     1441 cgacggccgt aatctgggca acgccaacca gacgctgacc ctgcccaccg tgacccatcg
     1501 ggtcgagatc gcgctggatg gatacgcggg ttactccacg gagataacgc cgcgcaacgg
     1561 ggtcacccag gaaatcaagg tgcgccttct gaccctggaa gaggctcgtc tggcagccat
     1621 gaaaccggtc atccagacca gccagggcca ggaactcgtg ctgctgaatc cgggcccctt
     1681 caccatgggc gcttcccggc gcgagccagg ccggcgagcc aacgagacgc tccgagaggt
     1741 gggcatggag cggattttct atctcagccg gaccgaggtg accaacgcgc agtttctcga
     1801 tttcgcgccc gggcatgact cgggggaatt tcaggatcac gagctcaaca aggatgatca
     1861 gccggtggtt ggcgtctcct ggcaggaggc cgccctctac tgcaactggt tgtcggccag
     1921 ggaagggtta cccccgttct atgccacgga ccttggcaaa gtggtcgcaa cgaaccccgc
     1981 ggctaccggc taccggttgc ccaccgaagc ggagtgggag tgggcggccc gccagaacgg
     2041 cccggacagc accggatatc gtttcccgtg gggaaccgcg ctgccgcccc ccgatcgtca
     2101 cggcaactac gccgaccggt cggccgcaca cctggtgggg cgcgtggtgt tcggctacaa
     2161 cgacaaccac atcgtctccg ccgagaccgg gacgttcgcg cccaacgcca tcggcatcta
     2221 tgatctgtcg ggcaacgttg cggagtgggt gaacgacttc tacgagattc cgggggctga
     2281 accgacgaag gatcccctgg gaccggacac cggggagtat cgcgtgatca gaggatccag
     2341 ctggatgaac ggaaccatca ctgacctgcg cctgtcgttt cgcgactacg gtgttgatgg
     2401 tcgcgaagac gtcggttttc gcatcgcgcg gttcgcggag ccgtcgtgaa aggccctctc
     2461 cttttcgcgg cggcagcgct gcttgcgtcg accatgggca ccgggttcgc cgtggctgcc
     2521 gaggaagctg ccgacacccg cgatcgcgcc ggggaagaag aggaggcgga cggcgagccc
     2581 agccccgaga tcttcgtgcc cagcgaggag atttcggagg acttcgcggt ctcattcccc
     2641 gtagacatct aatcgcgtct ggaaccacag caaatgaagc gtcagctacc ctttgaactc
     2701 atttaccagt cgtcagcgct ggtccttgcg gtgatcctcg ttcatctggt ctacgtcacg
     2761 gtcatcagac cgaatgccga cgccatcatc gccgcccagg ccgaacgtgc tgcagccggc
     2821 gaggcatacg cccaggaacg ctccctttat gtggtgctca aggactacga gcaggagtcc
     2881 tgtttcgtgc tgatgctctg ggccatggcc atcatggggt ataagacccg cagcgcgttg
     2941 cgcgagcgaa gcctgctgca ggaagacatc gtcaagatca gtgaaggcat gagcgttctc
     3001 ccggaggaca ctcgggagta cgcgcgggcc gtccaggcac tgccggacga gaatcgaaac
     3061 tacctgctgc cgagggccct gatggctgcc ctgcagcggt ttgcctccac ccgtaacgtg
     3121 caggacgtct ccgaagccgt gcggtcggtt tgcgagtcag agtccgatcg gctcgacagc
     3181 gagctgtcca tggtccgcta catcgcgtgg gcaattccct ccatcgggtt catcggcacg
     3241 gtaaggggta tcagcgaggc tctcgggcag gcctacgagg ctgtcgaggg caacattgcg
     3301 ggcgtcacca cgagcctggg cgtcgcgttc aactcaacct ttaccgcgct ggtgatcagc
     3361 atcgtgatca tgttcctcat gcatcagctc cagctcatcc aggagcgact ggttctggac
     3421 gcccagactt actgcgacga gaggctgctg cgtcatcttc aggtgcgcga ctgatggact
     3481 acgtcctcga gctcaacgac tcggacctgt cgctgcactc gggcgagacg ctggcctatc
     3541 acgcgccggg agtcgctctg gtccgggaaa acgagatcct cttcggcgac cccgcgcttc
     3601 ggctgtcccg cgcacacccc cgccagatca accagcacta cctcgcccgc atgaacgcgg
     3661 acccgctccc ctaccccgtt tccaacgccg ctaatcacgc ggatctggtc tacctgcacc
     3721 tcaagcagct tcaggacctg atgcggcgcg acccgggaga tctcgtgctg gcggtacccg
     3781 cgatcctgag cgcagatcag ctcggcgtgc tgctgggcat aacccaggag tgcggcatgg
     3841 cggtgagcgg cttcgttgac tccgcagtag cagcggcgtc ggtcagcgag ctgccggcca
     3901 gcgtctgcca tctcgacgcg cacctgcaca ggacgtgtct taccctgctg gatgtcggcg
     3961 ccgacgtcgt tcgaacgggg gcggatgagg ttgccgactg cggcgtggcc aacctgctgg
     4021 aaggctgggt caacgtgatt gccgaccgct tcgttcggga cacgaggttc gaccctctgc
     4081 acgccgccga gacggagcag caactctaca atcaggtcta cgactgggcc atgggccagt
     4141 cgagcagcga cagcgagatc ggcatcgaga tcggctaccg ggatcaggtc aggcgggtag
     4201 aggtctcccg cagtctgctc gaggagaaag cccggcagcg attcgcccac ctcagcgaac
     4261 gcctccccga ggacgtgcac ctggcgttga gcgcgcgtac ggcaaggctc cccgggctga
     4321 ccgcctatct gggcgcccag ggttgggaat actcggtact cgacggctcc gccctggcgg
     4381 cgggctgcag tgcgaacgac tccagcatcc gggccgagga cggccggctc aggctggttt
     4441 cccggctgcc attacggcac ggccggggtc cgcagagcat cgttgccccg gggcccgacg
     4501 agtccggccg ccctacccac gttctctgcg atgcctgcgc cgtgcccatc ggtgccgacg
     4561 atctgccgct gcagctcaag gagagcgagg ccggcgtgta tctcgatgcc gacgagacgg
     4621 tccgtctcaa cggcgaggcg atcaccacca gcagacgcct gcgcgcgggg gatgagctca
     4681 ccgccggcgg caaaagctat ctcctgatcc gcgtggtggg gtgaggtggc ccgcaaccgg
     4741 cgcttcagcg tcttctctct gtcgttttta gacgtgatgt cgtgcgggtt cggggccgtg
     4801 gtgctgatct tcctgatcat caatcacgca gccgaggagg acgccaaggt cgtcaacgag
     4861 gaactccttt cagagattcg cctgctcgac tatcaggtgc agaacggtga gcgggacctt
     4921 ttcgagctca aagagcagct cgacgccatt ctgcagcggc tgtccgactc gagccgcaag
     4981 ctcgtcagca cggaaaccag cctgaaggaa accagcaaag agttcgccga cctcgccgcg
     5041 gagtcccttg cccaggaagc tagcctggaa gagctgaaga gcgacgtgga cacgcgcgag
     5101 gaggagctgg ccaggctcaa atccatcaac gaggcaaacg agggaggcag gatacgcagc
     5161 ttcaccggcg agggggatcg gcaatacctg actggcctga aggtcggcgg caagcacatc
     5221 ctcattgccg tggacacgtc gaccagcatg ctcgatgaga gcatcgtcaa cgtgattcgg
     5281 cgccgcaaca tgcccgacga tcgcaaacgg atgtcgccca agtggcaacg ggccgttcgc
     5341 accgtcgagt ggctgaccgc ccagctgccc cttgacgcca gtttccagat ctacggattc
     5401 aacgtggagg cgcgatcgct gctggaagag caccatggac agtggattgc tcttgccgag
     5461 ggccgcgagc tggacgacgc gctggaagct ctgcgcgagg tgatacccga ccggggaacc
     5521 agcgtcgcca acctgatcag cgccgtcaac gagctgaatc cgccgccgga caacgtttat
     5581 ctgatcatcg actccctgcc gacccagggc acccgcgagc cgcgccgggc caccatctcc
     5641 ggcggcgatc ggctgaa
//
LOCUS       DBNHKV010000201         6901 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_523803, whole genome shotgun sequence.
ACCESSION   DBNHKV010000201 DBNHKV010000000
VERSION     DBNHKV010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6901)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_523803"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="AC16PP_06400"
     CDS             complement(<1..838)
                     /locus_tag="AC16PP_06400"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR6481293.1"
                     /translation="MQFRPRRSVLYMPGANARAMEKARELPCDAIIFDLEDAVAVDAK
                     VAARDQVAQAVSAGGYGYRELIVRVNGLDTEWGRDDVAAAARLPVDALLFPKVESPEQ
                     VATIVAAVDAAGGKALPVWLMIETPAGVLDAPAVACGSPRVRCLVMGTSDLVKELRGR
                     HTRTRSNLNFALQRCVLSARSAGVDILDGVHLDFRNAETFRDACEQGRDMGFDGKTLI
                     HPTQIEAANEAFGYSAADVDHARAVIAAWESAQSEGKGVAVLDGQLVENLHAAEAQRV
                     LAF"
     gene            complement(851..1258)
                     /locus_tag="AC16PP_06405"
     CDS             complement(851..1258)
                     /locus_tag="AC16PP_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481294.1"
                     /translation="MLYLNGAIGCIFIVIALLHAPHPDTFSWAPYLGAALLALISCRR
                     EVSMGVARALAVLTMVAMFYFFAAFFVHVPRLAADWYMHQEGWSAVCEILAAFAMIPI
                     LSDFSCRLKAECREARAARRAFFSVPSNIQPHR"
     gene            complement(1611..2165)
                     /locus_tag="AC16PP_06410"
     CDS             complement(1611..2165)
                     /locus_tag="AC16PP_06410"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6481295.1"
                     /translation="MRVNEPRIPALEQSEWSEAAQEIMGPMVAQDRVFNIFRTLSNHP
                     DLMRRWLVFANHILGKSTLPVRERELVILRIGYLCQSGYEWGQHVRIAQEAGMTAQEI
                     RSAKTGPDTPGLSDLDRLLLKATDELHADAHVSDETWKALTGHLNTQQLMDLVFTVGQ
                     YNMVSMVLNSFGVQLDEGLPGWDI"
     gene            complement(2242..3447)
                     /locus_tag="AC16PP_06415"
     CDS             complement(2242..3447)
                     /locus_tag="AC16PP_06415"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF005600.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR6481296.1"
                     /translation="MTQLDDITQARQRICGQIELTACSRSATLSAMTGADVHLKFENL
                     QFTASFKERGALNRLLQLTGEQQRFGVVAMSAGNHAQALAYHGKRLGVPTCIVMPRFT
                     PNAKVEQTRVHGAEVLLHGAEFDETLAFTEALAEERGLTLIHPFDDPAVIAGQGTLGL
                     ELVEQVPGLEAVRVPVGGGGLIAGVATAVKSVKPEVELLGVQVERFASVYAAFHGREV
                     PPGPPATVAEGIAVRAPGKNTLPLIRRHVDDVVLVSEEQVEQSIFKLLEIEKTVVEGA
                     GATSLAALLAEPGRFAGRRVACVLSGGNIDMMMLSSVVQRGLVRSRRLVRLSVEIPDV
                     PGALSELTRVLGALDSNIVDIAHQRAFGGSSVRATRVDLVLQMRGEEQVDEVVQELQA
                     RGYECARGG"
     gene            3516..3977
                     /locus_tag="AC16PP_06420"
     CDS             3516..3977
                     /locus_tag="AC16PP_06420"
                     /inference="COORDINATES: protein motif:HMM:NF015325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR6481297.1"
                     /translation="MKHLLIVYHTQTGRTGQLAAAVHRGATNELVSDVDARLLTAQNA
                     GADDLLWADGLLLGTPENFGYMSGGMKDFLDRTFYPVEGKILSLPYAIFISAGNDGSG
                     ALRAIRRIANGYPFKEVQEPVIARGEITSDHIDRCDELGLTLAAGLEAGIF"
     gene            4044..5252
                     /locus_tag="AC16PP_06425"
     CDS             4044..5252
                     /locus_tag="AC16PP_06425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6481298.1"
                     /translation="MTSRTARILHSIDEIPAEDWNRCAMPGGEIPHDPFLRHEFLAAL
                     EHSGSAAARTGWQPFHLVLEEADRILGVVPMYVKNHSQGEYVFDYAWADAWHRAGGDY
                     YPKLQVSVPFTPATGRRVLAAGQDEVSRPEIERALLGACAQVAAEIKVSSLHVTFLPE
                     AQWQIAGELGFLRRMDQQFHWHNAGYGSFEEFLGDLSSKKRKNLRRERRDALATGIRI
                     EWLTGSDIQESHWDAFYRFYMDTGSRKWGSPYLTRQFFSLVTETMAEDVLLIMCSRDG
                     RYIAGALNFIGGEALFGRNWGCIEHHPFLHFEACYYQAIDFAIERGLKRVEAGAQGPH
                     KVARGYLPCPTYSAHWIADAGFRNAVARYLDEEREHVREDIEYIEERSPFKSTVSLDK
                     FHNTRTLDIG"
     gene            complement(5278..6054)
                     /locus_tag="AC16PP_06430"
     CDS             complement(5278..6054)
                     /locus_tag="AC16PP_06430"
                     /inference="COORDINATES: protein motif:HMM:NF022665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HIR6481299.1"
                     /translation="MRNLIIVIALVLVLLGGLWLASDRSAGDREVPLPGAEPVPVPGP
                     RPAEPARRPQPMPVAEPAIVLPDLDASDPFLRERLAGVELPQNWIDRDDLVRRLAVVI
                     ENAARGEYPRRQLGFLAPSGRFRVVERQGRLFVDPDNYRRFDGYLDVLEGLDPRTAAE
                     LLSLIEPLLAVAMGELGSDGSVSDQIDAAVAEVMAVPDVVGEVELAQPKVLYEYADPA
                     LEALSPLQKQVLRMGPENAARLKRYVAEMAVALQTDPSDG"
     gene            6152..>6901
                     /locus_tag="AC16PP_06435"
     CDS             6152..>6901
                     /locus_tag="AC16PP_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR6481300.1"
                     /translation="MTDTIKDILAHRLPVGAEVTIEGWVRSRRTSKGGFSFVHVSDGS
                     CFDTIQAVADSQLANYEHDVEHLGTGCSVRIRGTVVESQGRGQDREIQATEVVVVGWV
                     DDPETYPIAKKRHTFEYLREVAHLRPRTNTFGAIARVRNAIAQAVHGYFNDNGFVWVN
                     TPIITASDCEGAGQLFRVSTLDIANRALTRGDSPFDDDFFGRETFLTVSGQLNLESYC
                     LALSKVYTFGPTFRAENSNTSRHLAEFWMIEP"
BASE COUNT         1302 a         2360 c         2076 g         1163 t
ORIGIN      
        1 cgaacgcgag cacccgctgc gcctctgccg catgcaggtt ctcgacgagc tgcccgtcga
       61 gcacggcaac ccctttgcct tcgctttgcg cggattccca tgccgcaatg accgcccggg
      121 catgatccac gtctgccgcc gagtagccga aggcctcgtt ggcggcctcg atctgcgtcg
      181 ggtgaatcag cgtcttgcca tcaaatccca tgtcgcggcc ctgctcgcag gcatcacgaa
      241 acgtttccgc attgcggaaa tccagatgca ccccgtcgag gatgtcgacc cctgccgatc
      301 gggccgagag cacgcaacgc tgcagagcga agttcagatt gctccgggtt cgcgtgtgcc
      361 gccctcgcag ttccttgacc aggtcactgg tccccatgac aagacaacga accctcggtg
      421 acccacaggc caccgccgga gcgtccagga cgccggccgg ggtttcgatc atgagccaca
      481 ccggcagcgc tttaccgcca gcggcatcca ccgcagctac gatggtcgca acctgctccg
      541 gcgactcgac cttgggaaag agcagcgcat ccaccggcag gcgggctgcg gctgcaacgt
      601 catcacggcc ccattcggta tccaggccgt ttactctcac gatgagttcc cgatacccgt
      661 agccgccggc actcaccgcc tgggccacct gatcccgagc agcgaccttg gcatccacgg
      721 ccaccgcatc ttcgagatcg aaaatgatgg catcacaggg aagttcccgc gccttttcca
      781 tggcgcgagc gttggcgccg ggcatgtaaa gcacggatcg gcgaggccgg aactgcatga
      841 ttacgcgttg ctagcgatgg ggctggatgt tgctcggcac cgagaagaac gcacggcgag
      901 ccgcgcgggc ctcacggcat tcggctttca gacggcagct gaaatcggaa agaatcggga
      961 tcatcgcaaa cgcagcgagg atttcgcaga ccgcgctcca gccttcctga tgcatatacc
     1021 agtcggctgc caacctgggc acgtgcacga agaaagcggc aaagaagtag aacatggcca
     1081 ccatggtcag caccgcgagc gcccgggcca cccccatact gacttcacgg cgacaggaga
     1141 tcagagccag cagagcggcg ccgaggtaag gggcccagct aaaggtgtcc ggatgcggag
     1201 cgtggagcag cgcgatgacg atgaagatgc aaccgattgc cccgttaaga tacagcatgc
     1261 cctgcgacac tccctttcat gtcattgtca cacctgtgag cacggcacgg ctctctgtct
     1321 ggttgtctgc ccgtcgagca gcgcttttca ggcacttgcc caggcattct cgtccttccg
     1381 cccgttgacg gcggggacgt ccggagcctt tgtgtttatg tgctacagcc acctgaatag
     1441 tccaaaatag cccttatcca gtcaaccact ttgttaccga tggtgacaaa acggctaacc
     1501 atctacataa gttagaccgt gcgggtccct ttaggtttgc ggcaagatag gggtttcggg
     1561 tttttgagca gcctttttta cagaatagcc gtcaactccc caagcccacc ctaaatgtcc
     1621 cagccaggca gcccctcgtc cagctgtacc ccgaagctgt tgagaaccat ggaaaccatg
     1681 ttgtactgcc ccacggtgaa gaccagatcc atgagctgct gggtgttcag atgcccggtg
     1741 agcgccttcc aggtctcgtc gctcacgtga gcgtccgcgt ggagctcatc ggtggccttc
     1801 aacagcaggc ggtccagatc ggaaaggccg ggagtgtccg ggcccgtttt cgcagagcgg
     1861 atctcttgcg ccgtcatccc cgcttcctgc gcgatccgga cgtgctgccc ccactcgtac
     1921 cccgactggc acagatagcc gatcctgagg atcaccagct cgcgctcacg gacgggcaag
     1981 gtggacttgc ccaggatgtg attcgcgaac accagccagc ggcgcatcag gtccgggtga
     2041 ttggaaagcg tcctgaagat gttgaagacc cgatcctggg caaccatcgg ccccatgatc
     2101 tcctgagcag cctcgctcca ctcggattgc tcgagcgcag gtatcctggg ttcgtttacg
     2161 cgcatgacgc ctccccgaaa gttctctggt tccgattgcc ccgtcaatat aacccgacag
     2221 acgatggcgc gccgccggga ttcacccgcc gcgggcgcat tcgtagcccc gcgcctggag
     2281 ctcctggacg acctcgtcca cctgctcctc accgcgcatc tgcagcacca ggtctacccg
     2341 cgtggcccgc accgatgagc cgccgaaggc tcgctgatgg gcaatgtcga cgatgttgct
     2401 gtccagggcg cccagcaccc gcgtgagctc gctgagcgcg cccggcacgt cgggaatctc
     2461 cacgctcagc cgaacgagcc gccggctgcg aaccagaccg cgctgaacga ccgacgacag
     2521 catcatcatg tcgatgttgc cgccgctgag cacgcaggcc acccggcggc cggcaaaacg
     2581 cccgggctca gccagcagag cggccaggct ggtggcgccg gccccctcga ccacggtttt
     2641 ctcgatctcc agcagcttga atatgctctg ctccacctgc tcctcggaga cgaggacgac
     2701 atcgtctacg tgccgcctga tcagcggcag ggtgttcttt cccggggcgc gcaccgcgat
     2761 gccttcagca accgtcgcgg gtggccccgg cggcacttcg cggccgtgaa aggctgcata
     2821 gaccgaagcg aagcgttcca cctgcacgcc gagcaactcc acctccggct tcaccgattt
     2881 caccgccgtc gccaccccgg cgatcaggcc cccgcccccc accggaaccc gcaccgcctc
     2941 gagtcccggg acctgctcca cgagctccag ccccagggtg ccctggccag caatgaccgc
     3001 cggatcgtcg aaggggtgaa tgagtgtgag gccgcgctcc tcggccaggg cttcggtgaa
     3061 cgccagcgtc tcgtcgaact cggcgccgtg cagcagcact tccgcgccgt ggacgcgcgt
     3121 ctgctcgacc ttcgcgttcg gagtgaaccg gggcatgact atgcaggtgg gtacgcccag
     3181 ccgcttgccg tgataggcca gggcctgggc gtgattgccc gccgacatgg ccaccacgcc
     3241 gaagcgctgc tgctcgcccg tcagctggag caaccggttg agcgctccgc gctccttgaa
     3301 cgacgccgtg aactgcaggt tctcgaactt gagatggacg tccgcgcccg tcattgccga
     3361 cagcgtcgct gagcggctgc aggccgtcag ctcaatctgg ccgcagatgc gctgccgggc
     3421 ctgagtaatg tcgtcgagct gggtcacgat cgaacttctg aaaaggcgtc agtatactcg
     3481 acgacccacc agcctcgccc acctcccgcc agcagatgaa gcatcttctc atcgtctacc
     3541 acacgcagac cggccgcacc ggccagctgg cggcggccgt ccacagaggt gccaccaacg
     3601 agctcgtgag cgacgtggac gcccgcctgc tgacggcgca gaacgcgggg gcagacgatc
     3661 tcctctgggc cgacggcctc ctgctcggca cgcccgagaa cttcggctac atgtccggcg
     3721 gcatgaagga ctttctcgac cggacgttct accccgtgga ggggaagatt ctttccctgc
     3781 cctacgcgat cttcatcagc gccggaaacg acggcagcgg ggcgctgcgc gccatccgac
     3841 gcatcgccaa cggatatccg ttcaaggaag tccaggagcc cgtcatcgcc cggggagaga
     3901 tcaccagcga ccacatcgac cgctgcgacg agctcggcct gaccctggcc gccgggctcg
     3961 aggccggtat tttctgaagc gttcgcctgc cagggcagcg caccttgcgg cgagcggtta
     4021 aactcggaac gaaggagaac cgattgactt cccgcaccgc gagaattctg cacagcatcg
     4081 acgagatacc ggccgaagac tggaaccggt gcgcgatgcc aggcggcgag attccccacg
     4141 acccgttcct gcgccacgag tttctggctg ccctcgagca cagcggcagc gccgcggcca
     4201 ggacgggctg gcaacccttt cacctggtcc ttgaagaagc cgaccgtatt ctgggcgtgg
     4261 tgcccatgta cgtgaagaac cactcccagg gcgagtacgt gttcgactac gcctgggctg
     4321 acgcctggca tcgggccggc ggcgactact accccaagct gcaggtcagc gttccgttca
     4381 cgccggcaac cggtcggcgc gtgcttgccg ccggccaaga tgaggtctcg cggccggaga
     4441 tcgagcgggc gctgctgggg gcgtgtgccc aggtagcggc agagatcaaa gtctcgtccc
     4501 tgcacgtcac cttcctgccg gaggcccagt ggcagatcgc cggcgagctc ggctttcttc
     4561 ggcgcatgga tcagcagttc cactggcaca acgcaggcta cgggagcttc gaggagtttc
     4621 tcggcgatct ctcctcgaag aagcgcaaga acctccgccg cgagcggcgg gacgccctgg
     4681 ccaccggcat caggatcgaa tggctcacgg ggagcgacat ccaggaatcg cactgggacg
     4741 ccttctaccg cttctacatg gacacgggat cccgaaaatg ggggtcccca tacctcactc
     4801 ggcagttttt cagcctcgtc accgagacca tggccgagga cgtgctgctg atcatgtgca
     4861 gccgggacgg gcgctacatc gcgggcgcgc tcaacttcat cggcggggaa gccctcttcg
     4921 gtcgcaactg gggttgcatc gagcaccacc cctttctgca cttcgaagcc tgctactatc
     4981 aggccatcga cttcgctatc gagcggggac tgaaaagggt ggaagccggc gcccagggcc
     5041 cgcacaaggt cgcccggggg tatctaccct gccctaccta cagcgcccac tggatagccg
     5101 acgcgggctt tcgtaatgcc gttgcccgct atctggacga ggagcgcgag cacgtccgcg
     5161 aggacatcga gtacatcgag gaacgcagcc ccttcaagtc caccgtcagc ctggacaagt
     5221 tccacaatac ccgcaccctg gacatcggct gaacggcttg cgccgaaggg cgcccgccta
     5281 gccgtcggag gggtcagtct gcaacgcgac ggccatctcg gcaacgtagc gcttgagccg
     5341 cgccgcattt tccggaccca ttcgaagcac ctgcttctgc agcggcgaca gcgcttccag
     5401 cgccggatcc gcgtactcgt agagcacctt cggctgagcc agctccacct cgccgacaac
     5461 gtcgggcacc gccatcacct ccgcgacggc agcatcgatc tgatcggaaa ccgacccgtc
     5521 gctgccgagc tcgcccatgg cgacggccag cagaggctcg atgagagaca gcagctcggc
     5581 ggccgtccgg gggtccaggc cttcgagcac gtcgagatac ccgtcgaagc gtcggtagtt
     5641 gtcggggtcc acgaagagcc tgccctgtct ttccaccacg cggaaacggc cggacggcgc
     5701 aagaaaaccc agctgacgcc ttggatactc tccgcgagcc gcgttttcga tcaccaccgc
     5761 caggcggcgg acgagatcat cacggtcgat ccagttctgg ggcaactcga ccccagccag
     5821 ccgttcccgc aggaacggat cgctcgcgtc gaggtcgggc agcacgatag ccggctcagc
     5881 gacgggcatc ggctgcggtc gccgcgcggg ttcagcgggc cgcggtccgg gaaccggcac
     5941 cggctcggcc cccgggagcg ggacttcccg atcaccggca ctgcgatccg aagccagcca
     6001 gaggccgccg agcagaacca gcacgagggc gatgacgatg atgaggttgc gcatgtctca
     6061 ttatgaccac gacccgcggg gaattatcca aggccgggcc aattccatta tcattccaca
     6121 atccctcatt cctgcctgag cagccgcgcc gatgaccgac accatcaaag acatcctcgc
     6181 ccaccgcctt cccgtcggag cagaggtcac catcgaaggt tgggtcagat cgaggcgaac
     6241 gtcgaaaggc ggcttctcct tcgtgcacgt cagcgacggc tcctgcttcg acaccattca
     6301 ggcggtggcc gactcgcagc tcgccaacta cgagcacgat gtcgagcatc tcggcacagg
     6361 ctgctccgtg cggatcaggg gaaccgtggt ggaatctcaa ggccgcggcc aggaccgcga
     6421 gattcaggcc accgaggtcg tggtggtggg atgggtggac gaccctgaaa cttacccgat
     6481 cgcaaaaaaa cgccacacct tcgaatacct cagagaagtc gcccatctgc gtccccgcac
     6541 gaacaccttc ggcgccatcg ctcgcgtgcg taacgccatc gcccaggcgg tgcacggcta
     6601 tttcaacgac aacggtttcg tctgggtcaa cacgccgatc atcacggcca gcgattgcga
     6661 gggcgccggg cagctgtttc gcgtctccac gctggacatc gccaaccggg cactgacccg
     6721 cggggacagc cccttcgacg acgacttctt cggtcgcgag acctttctga ccgtctcggg
     6781 gcaactgaac ctggaaagct actgccttgc tctgagcaag gtgtacacgt tcggcccgac
     6841 cttccgcgcg gagaactcga ataccagccg acatctggcc gagttctgga tgatcgaacc
     6901 g
//
LOCUS       DBNHKV010000202         6792 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1303131, whole genome shotgun sequence.
ACCESSION   DBNHKV010000202 DBNHKV010000000
VERSION     DBNHKV010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1303131"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1738
                     /locus_tag="AC16PP_06440"
     CDS             146..1738
                     /locus_tag="AC16PP_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6481301.1"
                     /translation="MNTKPLDAIVVGAGFAGLYMLHRLRGLGLKTRVFEAGSDIGGTW
                     FWNRYPGARCDIESMEYSYQFSDELAREWEWSERYATQPEILGYINHVAERFDLRGDI
                     QLNTRVERARFSEETNRWTVTTSDGGEHEAKYLVMATGCLSSTNLPEFDGIDSFQGDW
                     YHTGQWPHEGVDFTGKRVAVIGTGSSAIQSIPLIAEQAEQLTVFQRSPNFSVPAHNRA
                     LDPGAVARIKARYPEFRDANRQTAFHADFRYNESNALDVSAEQREREYEARWERGGLP
                     FLAAFADLVFDADANETAAEFIRGKIRETVKDPDTAELLCPTSVVGCKRLCVDTDYYA
                     TFNRENVTLVSVRDTPIEAITPKGLRTADEEYEFDAIVFATGFDAMTGALMKMDIRGI
                     GGRSIQDAWSEGPKTYLGLAVSGFPNLFTVTGPGSPSVLTNMLPTIEHHVDWIADCVA
                     YMEHEGMSRIDAMPEAQTAWVAHVNEVGDSSLYPRCNSWYLGANIPGKPRVFMPYLGF
                     PPYAEKCREVAESGYEGFALSA"
     gene            complement(1758..2939)
                     /locus_tag="AC16PP_06445"
     CDS             complement(1758..2939)
                     /locus_tag="AC16PP_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6481302.1"
                     /translation="MQVSRALIVGGGIGGLTAALCLARQGIAVSVFEQTADLAEVGAG
                     IQLSPNASRVLHHLGLEGALGSVAFLPRAVEMRDWRSGRLLASSPIDEAFRDRFVYPY
                     YHVHRADLLDVLERALRRAPGIELRTGARVEGIEPGDSAVFVEAGGERHGGDLLIGAD
                     GIHSTVREALFGAEAPRFTGNVAWRGLVPAGAVPSGFVRPAASAWWGPHKHFVHYYVR
                     RGELINCVGVVEKRGWEVESWTEPGDLSELQADFAGWHETVRRLIDNMDPQACFKWAL
                     FDRPPMSAWSDGRVTLLGDACHPTLPFMAQGAAMAIEDAAVLARCLVEGDGVSGSLRR
                     YESLRRARTARVQAGSRRNARLFHSSGPAAGLRNLIVGRASGRVMDWLYGYDALDPGA
                     P"
     gene            3128..3301
                     /locus_tag="AC16PP_06450"
                     /pseudo
     CDS             3128..3301
                     /locus_tag="AC16PP_06450"
                     /inference="COORDINATES: protein motif:HMM:NF046279.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
     gene            3281..3766
                     /locus_tag="AC16PP_06455"
     CDS             3281..3766
                     /locus_tag="AC16PP_06455"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024802.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6481303.1"
                     /translation="MAEYLDEVVPPRLHPEDALKRARNRAWTDFVPTFAAGLSALYYA
                     KSPEGHEERMTVARQRLDKIEEAIRAERGNEGPFFNGPDLSLVDAAYAPFFQRFLIVE
                     QWLATGLLESYPLVGAWADALAASPAVANSVPDVFHDEFVANLKRRGFHVAALMDAAA
                     G"
     gene            complement(3809..4906)
                     /locus_tag="AC16PP_06460"
     CDS             complement(3809..4906)
                     /locus_tag="AC16PP_06460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6481304.1"
                     /translation="MHVGFAPVFQNLGKPVPDHQVYAQELAMARAAEPMGFDSVWSVE
                     HHFTDYTMVPDVTQFLSYMAACTERVQLGSMVIVLPWHDPVRVAEEIAMLDALSGGRM
                     ILGIGRGLGRVEFDGFRVPMDESRERFVESATMILEGLERGFVELDGKHFQQPRRDIR
                     PAPFRTFRGRTFAAAVSPESMPVMARLGVGLLVIPQKPWESVRTDFEVYHRVWAETHG
                     AQSSPPDPMCGGFFFVDESADRAEEMAYKYIGRYYHSVMDHYELRIGHLEGTKGYEFY
                     EKIHEHIETRSDDGAARDFVRLMPFGTPEQVIEKVEFIHRMIGAKGVMTHFAYGGMDY
                     AEANRNMRLFADRVMPELQRLGEQRGALAVA"
     gene            4993..6561
                     /locus_tag="AC16PP_06465"
     CDS             4993..6561
                     /locus_tag="AC16PP_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6481305.1"
                     /translation="MAKGERRQASMYPGSWAQIKGEDVAVLAAGSGDALTWRDLDRRS
                     NQAAHALRGAGLGAGDHLALIMENHLQFFEIAWAAIRSGLYITCVNRYLTAEEAAYIV
                     TDSSAKAVIVSAEIGLGSKLLPLIAECPVRLMARGVEPGWTSYEKTIEACPFTPVADE
                     TVGDSMLYSSGTTGRPKGIKRPLTGQSPAKGIPGVEQMSPYGFSQESVYLSPAPLYHA
                     APFAFCMRTLSLGGTVIMMERFDPLEALGCIERYRVTHSQWVPTMFVRLLNLDDEART
                     GFDLSSHRCAVHAAAPCPVPIKQQMMTWWGPIIWEYYAGTERNGSTLIDPHDWLAHPG
                     SVGRARSGVIHICDDDGRELDPGQEGLIYFEQPERPFEYHNAPDKTANATHPDHGNWT
                     ALGDVGYLDDEGYLYLTDRKAFVIISGGVNVYPREIEDVLIVHPKVADVAVFGVPNEE
                     FGEEVKAVVQPAPGTSPGDALAAELVGHARAHLAGFKVPRSIDFIDEMPRLPTGKLYK
                     QPLRARYWPERKST"
     gene            6558..>6792
                     /locus_tag="AC16PP_06470"
     CDS             6558..>6792
                     /locus_tag="AC16PP_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481306.1"
                     /translation="MTGNSSILSDLPATAGVARSTRREGVDVSLNAPTPAQPAIASPE
                     WAVAGWIIRFVRLMAGHRLAIPQEPGDVFLKVVA"
BASE COUNT         1250 a         2332 c         2135 g         1075 t
ORIGIN      
        1 cgatcgaccc ggcccgggtg taaaaggctt gcacgtcatg ggttaagatt tatcaagagg
       61 acattcgccg cgatgtccct gccggacggc cgcgttcacg gcatccgggc agcgctgcga
      121 ggcaacagca gggggaagaa aggaaatgaa cacaaagcca ctcgacgcca tcgtggtggg
      181 ggccggcttc gccgggctct acatgctcca ccgcctgcgc ggcctgggcc tgaaaacccg
      241 cgtcttcgag gccggcagcg acatcggcgg aacctggttc tggaaccgat acccgggcgc
      301 gcgctgcgac atcgagagca tggagtactc ctaccagttc tccgacgagc ttgcccggga
      361 atgggagtgg tcggagcgtt acgctaccca accggaaatc ctcggctaca taaaccacgt
      421 tgccgaacgc ttcgatctgc gcggggacat acagctcaac acccgggtgg agcgagcccg
      481 gttcagcgaa gaaacaaacc ggtggaccgt cacgacgagc gatggcggcg agcacgaagc
      541 aaagtacctg gtaatggcaa ccggctgcct ttcctccacg aacctgcccg aattcgatgg
      601 catcgacagc tttcaaggcg actggtacca caccgggcaa tggccccacg aaggcgtgga
      661 tttcaccggc aagcgtgtcg ccgtgatcgg taccggctcg tcggccatcc agagcatccc
      721 gctcatcgcc gagcaggcgg agcagctcac cgtcttccag cgctccccca acttctccgt
      781 gcccgcgcac aatcgggccc tggatccggg agcggtcgcc cggatcaagg cccgataccc
      841 cgagttccgg gatgcgaatc ggcagacggc ctttcacgcg gatttccgct acaacgagtc
      901 caacgccctg gacgtatccg ccgagcagcg cgagcgcgag tatgaggcgc gctgggaacg
      961 gggcgggttg cccttcctgg ccgccttcgc cgacctcgtg ttcgacgccg acgccaacga
     1021 gaccgccgcc gagttcatca gaggcaagat acgcgagacc gtcaaagacc cggacaccgc
     1081 cgagcttctc tgcccgacca gcgtcgtggg gtgcaaacgc ctgtgcgtgg atacggacta
     1141 ctacgccacc ttcaaccgcg aaaacgtcac cctggtgagc gtgcgggaca cgcccatcga
     1201 ggccatcacg cccaagggac tgcgaacggc tgacgaggag tacgagttcg acgccatcgt
     1261 gttcgccacc ggcttcgatg ccatgacggg ggcgttgatg aagatggaca ttcgcgggat
     1321 cggcggccgt tcaattcagg acgcatggtc ggaaggcccc aagacctacc tgggcctggc
     1381 cgtgtccggt tttccgaacc ttttcaccgt caccggcccc ggaagcccct ccgtgctgac
     1441 gaacatgctc ccgacgatag aacaccacgt cgactggatc gccgactgcg tcgcctacat
     1501 ggagcacgaa ggcatgtccc gcatcgacgc gatgcccgag gcccagacag cgtgggttgc
     1561 tcacgtgaac gaggtggggg attccagcct gtatccacgc tgcaactcct ggtatctggg
     1621 tgcgaacatt ccgggcaagc ctcgcgtgtt catgccctat ctgggttttc cgccctacgc
     1681 cgaaaaatgc cgggaggtgg cggaatccgg ctacgaaggt tttgcgctga gcgcgtgaca
     1741 gaacccggcg gcgcccatca tggcgcccca ggatccagcg cgtcgtaccc gtaaagccag
     1801 tccatgacgc gacccgaagc gcggcccacg atcaggttgc ggagcccggc agccggcccg
     1861 ctcgagtgga agagccgcgc gttccgcctg gaaccggcct gcacccgggc cgttcgcgcc
     1921 ctgcgcaacg actcgtagcg gcgcaggctc ccggaaaccc cgtcgccttc caccaggcac
     1981 cgcgccagca ccgccgcatc ctctatggcc atggccgcgc cctgggccat gaacggcagc
     2041 gtcgggtgac aggcatcacc caggagcgtc acccgtccat cgctccacgc cgacatcggc
     2101 ggacgatcga acagcgccca cttgaagcag gcctgcgggt ccatgttgtc gatcagccgc
     2161 cgaaccgtct cgtgccatcc ggcgaagtca gcttgcagct cgctgaggtc tccaggctcc
     2221 gtccaggact cgacttccca cccgcgcttc tcgacgacgc ccacacagtt gatcagctcg
     2281 cctctccgga cgtagtagtg cacgaaatgc ttgtgcggcc cccaccaggc gctggcggcc
     2341 ggccggacga agcctgaggg gaccgcgcct gccggcacca ggccccgcca ggcgacgttg
     2401 cccgtgaacc gcggcgcttc ggcgccgaac aacgcctcgc ggaccgtgga gtgaataccg
     2461 tccgcaccga tcagcaggtc accgccatga cgctcgccgc cggcctcgac gaagacggcg
     2521 gagtcgcccg gctcgatacc ctcgaccctc gcaccggtgc gcagctcgat acccggtgcc
     2581 cgccgcagcg cgcgttccag gacgtcgagc agatcggccc ggtgcacgtg atagtaggga
     2641 tagacgaacc ggtcgcggaa agcctcgtct atggggctag aggccagcag gcggccgctt
     2701 cgccagtcgc gcatctccac ggcgcgcggg aggaaggcga cgctgccgag agcgccctcg
     2761 agacccagat gatgaagcac gcgagaggcg ttgggcgaaa gctgaattcc cgcgcccacc
     2821 tcggcgagat cggccgtctg ctcgaacacg ctgacggcaa tcccttgcct tgccaggcag
     2881 agcgcggccg tgagtccgcc gatcccgcca ccgacgatga gcgcgcgcga tacctgcaac
     2941 ggtaatccat ggcatggctt cggcaccgat ggtataccgg caacttcgct cagaccaacg
     3001 cccgagtggt cggctgcaac ctcgcgtacg cttgtcaggt caccgcctga cggcgtagtc
     3061 ttcagccatc atgaacgcac ccgcgagata tcacctcatc agctccttca cgtgaccctg
     3121 ggtccagcgc gccgtgatcg tgatgcgcgc caaggacgtc gacttcgacg tgacctacat
     3181 cgacctgcgc gacaagcccg actggtttct ggagatttcc ccccacggca aggtgcccgt
     3241 gctgcaggta gacggggtcc cgctgttcga gtccaacgcc atcgccgagt atctcgacga
     3301 ggtggtcccc ccgcgcctgc atcccgagga tgcgctaaag cgcgcccgca accgtgcctg
     3361 gaccgatttc gtgcccacct tcgccgccgg cctgagcgcc ctgtactacg caaagtctcc
     3421 ggagggccac gaagaacgca tgacggttgc ccgacagcgc ctcgacaaga tcgaggaggc
     3481 cattcgcgcc gagcggggca acgaagggcc cttcttcaac gggcccgatc tctccctggt
     3541 ggatgccgcc tacgcgccgt tcttccagcg ttttctcatt gtcgagcaat ggctggccac
     3601 cggcctgctc gagtcttacc cgctggtggg cgcctgggca gacgcccttg cggccagccc
     3661 ggcggtcgca aactccgtgc cggacgtctt ccacgacgag ttcgtcgcca acctgaagcg
     3721 tcgcggattt catgtagccg ccctgatgga cgcagcggcg ggctgagcag ggaagcggcc
     3781 gcagcccgcg ggtcgcctga atccggcctc acgccaccgc caaggcccct cgctgctcgc
     3841 ccagccgctg cagctccggc atgaccctgt cggcaaacag acgcatgttg cggttggcct
     3901 cggcgtagtc catcccgcca taggcgaagt gggtcatcac gcctttggcc ccgatcatcc
     3961 ggtggatgaa ctcgaccttc tcgatcacct gctcgggcgt gccgaacggc atcagccgga
     4021 cgaagtcccg ggccgcgccg tcgtcggatc gggtttcgat gtgctcatga atcttctcgt
     4081 agaactcgta acctttcgtg ccttccaggt gcccgatgcg aagctcgtaa tggtccatga
     4141 cggagtggta ataacggccg atatacttgt aggccatctc ctcggcccga tcggcgctct
     4201 cgtcgacgaa gaagaagccg ccgcacatag ggtccggcgg agacgactgc gcgccgtgag
     4261 tttctgccca gacccggtgg taaacctcga aatccgttct gaccgattcc catggtttct
     4321 gcgggatgac aagaagccca acaccaagcc gggccatcac cggcatggac tccggggaca
     4381 ccgccgcggc gaacgtccgc ccccggaagg tcctgaacgg cgccggccgg atgtcgcggc
     4441 gaggctgctg gaagtgcttg ccgtcgagct ccacgaagcc gcgctccagc ccctcgagga
     4501 tcatggtcgc cgattcgacg aaacgctcac gggactcgtc catgggcacg cgaaagccat
     4561 cgaactcgac gcggccgagc ccccggccga tacccaggat catgcgcccg ccggagaggg
     4621 catcgagcat ggcgatttcc tcggcgacgc gaaccgggtc gtgccagggc agcacgatca
     4681 ccatggagcc gagctgcacc cgctccgtgc aggccgccat gtagctcaga aactgcgtga
     4741 cgtcgggcac catcgtgtag tcggtgaagt ggtgctccac cgaccaaacg gagtcgaagc
     4801 ccatgggttc cgcggcgcgg gccatggcca gctcttgcgc gtacacctga tggtcgggta
     4861 ccggcttgcc gaggttctga aatacgggtg cgaatccgac gtgcatgact ttcctccctc
     4921 tgcttaccgc aatttaacat cagccggaaa atccatgcga tccctttccc tgccgcccgc
     4981 gggacaggca tcatggcgaa gggcgagcgg aggcaggcca gcatgtatcc gggcagctgg
     5041 gcgcagatca agggcgaaga cgtggccgtg ctggccgccg gcagcgggga tgcgctcacg
     5101 tggcgcgatc tggaccggag gtcgaaccag gccgctcatg ccctgcgcgg ggccgggcta
     5161 ggcgccggcg atcacctcgc gctgatcatg gagaaccatc tgcagttctt cgagatcgcc
     5221 tgggcagcaa tccgctcggg tctttacatc acctgcgtca accgctacct gacagccgag
     5281 gaggcagcct acatcgtcac ggactcgtcg gcgaaagccg tcatcgtctc cgcggagatc
     5341 ggtctgggca gcaagcttct gccgctgatc gccgagtgcc ccgtgcggct gatggccaga
     5401 ggcgtagagc cgggctggac gtcgtacgag aaaaccatcg aggcctgccc cttcaccccg
     5461 gttgccgacg agaccgtggg cgattccatg ctctacagct cgggcaccac gggccggccc
     5521 aagggcatca agcggcccct gaccggccaa tcgccggcga aaggcatacc cggggtggag
     5581 cagatgagcc cctacgggtt cagccaggag agcgtctacc tgtcacctgc gcccctctac
     5641 cacgccgcgc cgttcgcatt ctgcatgcgg acgctgagcc ttggcggcac ggtgatcatg
     5701 atggagcgat tcgatccgct ggaggccctc ggctgcatcg agcgataccg ggttacccac
     5761 agccagtggg tgccgaccat gttcgttcgt ttgctgaacc tcgacgacga ggcgcgaacg
     5821 ggattcgacc tcagcagcca ccgttgtgcc gtccacgccg cggcaccctg cccggtcccc
     5881 atcaagcagc agatgatgac gtggtgggga ccgatcatct gggaatacta cgcgggcacg
     5941 gagcgcaacg gctcgaccct gatcgacccc cacgactggc tggcccatcc cggctcggta
     6001 ggacgggcgc gcagcggcgt catccacatc tgtgacgacg acggccggga gctcgacccg
     6061 ggccaggaag gcctgatcta cttcgagcag cccgagcggc cgttcgagta ccacaacgcg
     6121 cccgacaaga cggccaacgc cacccacccc gaccacggca actggacggc cctcggcgac
     6181 gtcggctatc tggacgacga gggttacctc tacctgaccg accgcaaggc gtttgtgatc
     6241 atctccggag gggtgaacgt ctacccgcgc gagatcgagg acgtgctcat cgttcacccg
     6301 aaggtggcgg acgtggccgt gttcggggtg ccgaacgagg agttcggcga ggaggtcaaa
     6361 gccgtggtgc agccggcgcc cggcacgagc cccggcgacg ccctggcggc ggagctcgtc
     6421 ggccatgcgc gcgcccacct cgccggtttc aaggtgcccc gctccatcga cttcatcgac
     6481 gagatgccga ggctgcccac tggtaagctc tacaagcagc cgctgcgggc acgttactgg
     6541 ccagaaagga aatcgacatg accggaaact cgagcatttt atccgacctg cctgccaccg
     6601 cgggagtcgc ccgcagcacc cgcagagaag gggtggacgt cagcctgaat gcgcccacgc
     6661 ccgcccagcc ggccatcgca tcgccggaat gggcggtggc gggctggatc atcaggttcg
     6721 tccgcctgat ggccggccac cgcctggcca tcccacagga accgggtgac gttttcctca
     6781 aggtggtcgc cg
//
LOCUS       DBNHKV010000203         5296 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_666381, whole genome shotgun sequence.
ACCESSION   DBNHKV010000203 DBNHKV010000000
VERSION     DBNHKV010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_666381"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1917
                     /locus_tag="AC16PP_06475"
     CDS             <1..1917
                     /locus_tag="AC16PP_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR6481307.1"
                     /translation="GQIRFEDVLDNLIQPARLVAGHAVSPGMIRFATRLSLRRRKAIP
                     REWMASATLCRVRFELRVKNWRSYIPILDVITHYRRSDFSRDLTSGLVVGVITIPQAI
                     AYAFLAGLPAQAGLYACLVPMVIYAILGSSRHLVVGPVAVAALMVAAAVGEHAPLYNE
                     SYLGITAVLGLQAGIFLWLLRLWKMGGVVNLLSHPVIVGFVNAAALLIIVSQLNAFTG
                     IADEGSKNPIDRLGQLVSKLGELHTTTTIIGVCCVVGLWLVRRWGVSVAELLVHPLGF
                     NLGDRQLIARLGPLLVASVAVIVVWAGDLNGTAGVATIGLVPGGLPSLTVPPFNWDMW
                     VDLAPSSAMIAVVAYVESFSIGTMIATRQRTRINAHQELIALGAANIGAAFTGAYPVA
                     GSFSRSSVNYQTGARTPVSSLVCAAVIILTLLFFTPLFTYLPHAALAAIVIVSVTSLL
                     DFRSFRTHWRIHREDSVTQMVTLASVLLFGVEAGLLTGVTLSIAFFIRQSSRPNVTLV
                     GRMGDTEQFRSARRYPVKTFEHVAAVRVDENLYFGNANQVENKLLKVVHRRPATKHLV
                     LVCSAINMIDVSGLEMLYRINQNLTDMGITLHLSEVKGPVMEQLAATDFISALAGTVY
                     FTTDQAMRDLAPRT"
     gene            1929..2822
                     /locus_tag="AC16PP_06480"
     CDS             1929..2822
                     /locus_tag="AC16PP_06480"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR6481308.1"
                     /translation="MTDELDTADWECVLEGPRGAMTEAGFVLTAVAIEHRIELCGRIS
                     RLLVPPSQLAQAMAELESYQQENRPLPGSVYSVPTVDGGWAGVLGYLMVIWLQPSLEA
                     NQAFGWDWREAGHLQAGLVAAGEWWRCITALTLHGDLGHIVANSLFGSLFGLYVGRYL
                     GSGFGWLLILASGALGNAMNAWIQPDAFSSIGASTASFGALGVSAAFVWRRGYYRNRD
                     WRRSLAPIFAGIALLAFTGTGGENTDVVAHFTGFAAGALLGAAAAAFDIRRLGVSGQY
                     LAGAAAITLVVTAWSFAGTVG"
     gene            complement(2823..3401)
                     /locus_tag="AC16PP_06485"
     CDS             complement(2823..3401)
                     /locus_tag="AC16PP_06485"
                     /inference="COORDINATES: protein motif:HMM:NF016270.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HIR6481309.1"
                     /translation="MSHITTTFLRGLGVVLPLSLTIWFVVWLAVTTESLLRSVFLVIL
                     PEGLYLPGLGLIFGIVMVYGVGVLAQIFFLQSLWRHARNLLDRIPLVKTVYTAISDFY
                     QFFSSGPADGAARVVSVDVSDDVSVIGFVTGPSPSFMDQGGSRRIAVYFPMSYQIGGY
                     TLMVPEDRVSPLDIGVEDAMRVILTASVRSRR"
     gene            3538..4578
                     /locus_tag="AC16PP_06490"
     CDS             3538..4578
                     /locus_tag="AC16PP_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6481310.1"
                     /translation="MKLGLALGYSGAYLNIPLKKVQLAERLGYDSVWTAEAYGSDAIT
                     PLAYIAAHTERIRLGTGVIQLAARTPANAAMAFATLDQLAGGNRAICGIGVSGPQIVE
                     GWYGQPWGRPYYRIKDYVAIMKKIFAREEPVTHAGKEISLPYQGEGALGIGKPLKSIL
                     HMNPNIPIFLGTGMESTVRLTAEVADGWLPLGFVPETAALYDGWIDEGLAKGGKTSEQ
                     FERQAGTSVQVTDDVKSALDRLKPGIALYVGGMGHKTKNFHKEMMIRRGFGEAAERIQ
                     ELYLAKRKDEAIAAVPDEFVDQGALVGPKERIARRFRDWEDSGLTGLTISGDEEAVRF
                     MAECARLNFAAE"
     gene            complement(4590..>5296)
                     /locus_tag="AC16PP_06495"
     CDS             complement(4590..>5296)
                     /locus_tag="AC16PP_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HIR6481311.1"
                     /translation="GTKLDTKHAVHSGWLQLRYRSGGVERSRPAASPRPSPRGFYLTT
                     FAGAGFSVDVEIDPGQTNFDALDVGPVAAFGVGYALSRRWRFELETAFRKNEVELLDF
                     GLRFGSKQGSGRIRSTSLLASGIYQFFPEARTRPYLGVGAGLVWADYDVDVLDELFVD
                     DKDQSYAAEFRLGAEVSVSQRLTVSAEYRYWISSDLKMTGPDGGPVEAYQQTHSMMVG
                     LRYALGSGRSAGASGR"
BASE COUNT          950 a         1748 c         1697 g          901 t
ORIGIN      
        1 gggcagatcc ggttcgagga cgtcctcgac aacctgattc agccagctcg cctggtcgcg
       61 ggccatgcgg tctctccggg aatgattcga tttgcgacga ggctatcgct tcgcaggcga
      121 aaagcaatac cacgcgaatg gatggcgtct gctacgcttt gccgtgtgcg cttcgagctg
      181 agagtcaaaa actggcgcag ctacattccg attctcgatg tgatcaccca ctatcgacga
      241 tcggatttca gccgcgacct gacgtcagga ctggtggtcg gcgtcatcac gatcccccag
      301 gccatcgcct acgccttcct tgcgggcctc cccgcacagg caggcctgta cgcgtgcctg
      361 gtgcccatgg tgatctacgc aattctcggg tcttcccgac acctggtagt ggggccggtc
      421 gcggtggcag cgctgatggt ggcggccgcc gtgggcgagc acgcgccgct gtacaacgag
      481 tcctacctcg gcattacggc cgtgctgggc ctgcaggccg gcatattcct ctggctgctg
      541 aggctttgga agatgggcgg cgtcgtcaat ctcctcagcc atcccgtgat cgtcggcttc
      601 gtcaacgcgg ccgcgctgct gatcatagtc agccagctca acgcgttcac cggcattgcc
      661 gacgaaggca gcaagaatcc gatagatcgc ctcggtcagc tcgtctcgaa gctgggcgag
      721 ctgcacacaa cgaccacgat cattggcgtg tgctgtgttg tcgggctctg gctggtacgg
      781 cgctgggggg tgtctgtcgc cgagctcctg gtgcacccgc tgggcttcaa cctgggcgac
      841 cggcaactga tcgcgcggct ggggccactc ctggtggcct cggtcgcggt gatcgtcgtc
      901 tgggcagggg atctgaacgg cacggcaggc gtcgccacca tcggcctggt tcccggcggt
      961 ctgccgtcac tcaccgtgcc gcctttcaac tgggacatgt gggtagacct cgccccgtca
     1021 tccgcgatga tcgcggtggt cgcctacgtc gaaagcttct ccatcgggac catgatcgcg
     1081 acgcggcagc gaacgcggat caatgcgcac caggagctca tcgcgctggg cgccgccaac
     1141 atcggcgcgg ctttcacggg cgcctatccg gtggccggga gcttctcccg atcaagcgtc
     1201 aactaccaga ccggagcgag aacgccagtc agctcgctgg tctgcgcggc ggtgatcatt
     1261 ctcacgctgc tgttcttcac cccgctgttc acctacctgc ctcatgcggc gctcgccgcc
     1321 atcgtcatcg tatccgtaac gagcctgctc gacttccgca gcttccgcac acactggcgg
     1381 atacaccgtg aagacagcgt gacccagatg gtcaccctgg cctcggtgct gcttttcggc
     1441 gtggaagccg ggctcctcac gggcgtcact ctttccatcg cgttcttcat ccgtcagtcc
     1501 agccgtccca acgtgacgct ggttggtcgg atgggggaca cggagcagtt ccgctccgca
     1561 cgacgctacc ccgtcaaaac gttcgagcac gtagcggccg tgcgcgttga cgagaacctc
     1621 tacttcggga acgccaacca ggtggaaaac aagctgctga aagtcgtgca tcgaaggccc
     1681 gccaccaagc acctggtact ggtgtgcagc gccatcaaca tgatcgacgt cagcggcctg
     1741 gaaatgctct accgcatcaa ccagaacctg accgacatgg gaatcacgct gcacctttcc
     1801 gaggtgaaag gccccgtgat ggagcagctg gccgccaccg acttcatctc ggcgctggcg
     1861 ggcaccgtct acttcaccac cgaccaggcc atgcgcgacc tggcgcccag gacctgagcg
     1921 ctagcggcgt gaccgacgaa ctcgacaccg cagactggga atgcgttctc gaagggcccc
     1981 ggggagccat gacggaggca ggattcgttc tgacagcggt ggccatcgag caccgcatcg
     2041 agctctgcgg acgcatttcc aggttactgg tccccccatc gcagctggcc caggcgatgg
     2101 cggagctcga gagttaccag caggaaaacc gaccgttgcc tggctccgta tacagcgtgc
     2161 cgacggtgga cggcggctgg gcgggcgtgc tcggctatct gatggtgatc tggctgcagc
     2221 cgtccctgga ggcaaaccag gccttcggct gggattggcg cgaagccgga caccttcagg
     2281 caggcctcgt cgccgccggc gagtggtggc gctgcatcac tgcgctcacc ctgcacggcg
     2341 acctgggcca catcgtagcc aactcgctgt tcggctcgct gttcggcctg tacgtcggcc
     2401 ggtacctcgg ctccggcttc ggctggcttt tgatcctcgc cagcggcgcg ctcggcaacg
     2461 ccatgaacgc gtggatccag ccggacgcgt tcagctccat cggcgcatcc accgcgagct
     2521 tcggcgccct gggcgtgagc gcggctttcg tgtggcgccg cggctattac cgcaaccgcg
     2581 actggcgccg cagccttgcg ccgatattcg cgggtatagc cctgctggcg ttcaccggca
     2641 ccggggggga gaacaccgac gtggtcgcgc acttcaccgg ctttgccgcg ggcgccctcc
     2701 tcggcgccgc agccgccgcg ttcgacatcc gtcgtctggg cgtctccggg cagtatcttg
     2761 cgggggccgc ggccatcacc ctcgtcgtga cggcctggtc gttcgccggc acagtcggtt
     2821 gatcaccgcc tgctgcgcac ggaggcagtc agaatcaccc gcatggcgtc ctctacgccg
     2881 atgtccaacg gcgacacccg atcttccggg accatgagcg tgtagccgcc aatctggtaa
     2941 ctcatgggaa agtagacggc gatccgccgg ctgccgcctt ggtccatgaa ggatggagag
     3001 gggccggtca cgaagccaat gaccgagacg tcatctgaga cgtccacgct cacgaccctc
     3061 gccgcgccgt cggccggccc gctggagaag aactggtaaa agtcgctgat tgccgtgtac
     3121 accgttttga ccagcggaat tcggtccagg agattccggg cgtgccgcca taggctctgc
     3181 aggaaaaaga tctgagcgag cacgccgacc ccgtagacca tcacgatgcc gaatatgagg
     3241 cccaggcccg gcaggtagag gccctccggc aggatgacca ggaacaccga gcgcagcagg
     3301 gactccgtgg tcacggccag ccagacgacg aaccagatgg tcagggaaag gggcagcacg
     3361 acgccgagac cgcgcaaaaa ggtagtggtg atgtggctca tgacgatacg accttgaagt
     3421 tgatgcagcg aagtctagcc gttcgcgcga cgccgcgttc aaccgcgccg atggtcggcc
     3481 cgacgggcgg ccgccacttg agttaagctc caggcaaaag ttgcgcagga gctgacgatg
     3541 aaactgggac tggctttagg gtattcgggc gcgtacctga acatcccatt gaagaaggtt
     3601 cagctggccg agcggctggg ctacgactcc gtctggacgg ccgaggccta cggttccgac
     3661 gcgataacgc cgctggctta catcgcggct cacaccgagc gcatccggct cggcaccggc
     3721 gtcatccagc tcgctgcccg tacgccggcc aacgccgcca tggcgttcgc cacgctcgat
     3781 cagctggcgg gcggcaaccg tgccatctgc ggtatcgggg tttccggccc gcagatcgtc
     3841 gagggctggt acggacagcc ctggggacgg ccctactacc ggatcaagga ttacgtggcc
     3901 atcatgaaga agatcttcgc ccgggaagag cccgtcaccc acgccggcaa ggagatcagc
     3961 ctgccttacc agggcgaggg cgccctgggg atcggcaaac cgctgaagtc gatcctccac
     4021 atgaacccga acatacccat cttcctggga actggcatgg agtccaccgt gcgcctgacg
     4081 gccgaggtcg ccgacgggtg gctgccgctg ggattcgtgc ccgaaaccgc agcgctctac
     4141 gatggctgga tcgatgaggg ccttgccaaa ggcggcaaga cgtcggagca gttcgagcgg
     4201 caggcaggta cgtcggtcca ggtgaccgac gacgtgaaaa gcgctctgga ccggctcaag
     4261 ccaggcatcg cgctgtacgt cggcggcatg ggccacaaga ccaagaactt ccacaaggag
     4321 atgatgatca ggcgcggctt cggcgaagcc gcagagcgga tccaggagct ctacctggca
     4381 aagcgcaagg acgaggccat cgccgccgtt cccgacgagt tcgtcgacca gggcgccctg
     4441 gtgggtccca aggagcgcat cgccaggcgt ttccgggact gggaggattc gggcctcacc
     4501 ggtctgacca tttccgggga cgaggaagcc gtgcgcttca tggccgagtg cgcgcggctg
     4561 aatttcgccg ccgagtgagc ctgcggcgcc tagcggccgg aagcgcccgc tgagcgcccc
     4621 gaacccaggg cgtaccgcag acccaccatc atcgagtggg tctgctggta ggcctccacc
     4681 gggccgccgt ccggccccgt catcttcaga tcgctcgaaa tccagtaccg gtattccgcc
     4741 gacaccgtca gccgctggga cacgctgacc tctgcaccca ggcgaaactc ggccgcgtaa
     4801 gattggtcct tgtcgtccac gaacagctca tcgagaacgt ctacgtcgta gtcggcccac
     4861 acgagccccg cgccgacacc caggtaaggg cgggtccggg cttccggaaa gaactggtag
     4921 atgccggacg cgagcaggct ggtggagcgg atccggcccg agccctgctt ggaaccgaac
     4981 ctgagcccga aatccagaag ctcgacctcg ttcttccgaa acgcggtttc cagctcgaag
     5041 cgccagcgcc ggctgagcgc gtaaccaacc ccgaacgccg ccaccgggcc cacgtccaga
     5101 gcgtcgaaat tcgtctgacc gggatcgatc tcgacgtcca cggagaatcc cgcgcccgca
     5161 aaggtggtga ggtagaagcc ccggggcgaa ggccgagggg acgctgccgg ccgcgaccgc
     5221 tcgacgccgc cggaacggta gcgcagctgc agccagcctg aatgaacggc atgtttcgta
     5281 tcgagcttgg tccctg
//
LOCUS       DBNHKV010000204         3152 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_381146, whole genome shotgun sequence.
ACCESSION   DBNHKV010000204 DBNHKV010000000
VERSION     DBNHKV010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_381146"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="AC16PP_06500"
     CDS             <1..370
                     /locus_tag="AC16PP_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481312.1"
                     /translation="FSMEIDLSVEGQIGDDTAIIAGGTETAGITAPLPNISMRGGHRF
                     GDNFYLGGTAGYFSLKVGDIDGELITARGSLDWRPHKRFGLGIGYQFVSFDVQEKKET
                     KQERYDADFYGPIFFVSAGF"
     gene            complement(420..1394)
                     /locus_tag="AC16PP_06505"
     CDS             complement(420..1394)
                     /locus_tag="AC16PP_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR6481313.1"
                     /translation="MSANCSDWLKAGLLCAALAVLGSCVTRAEQVASQSDMSWFARTV
                     SNSPLADADPDDFYEDASKMVRTDIEFHGVVLSEEGNPLKGARVRTSVFDRLADPFEF
                     PFFAFAAREPVFTGDDGRFSVRERGAGMYVTVEVPGWAPVTAPRKLYVYAEGLNQTLP
                     FPTREAPEEFRFERRPPEEELRPIQTGALRLPEDGVPLEVSLRQVAPYGVEPGTGEAI
                     ITCERTADDAAPDDLFDWWCELTVPGGGFQPFRIDMDQAPEEGYVESSRLEYRAADPR
                     WDDRAERSLIIRFADGMYGFIEVSMRMDGDFYVAFSGHWNPTGSTWLD"
     gene            complement(1375..3096)
                     /locus_tag="AC16PP_06510"
     CDS             complement(1375..3096)
                     /locus_tag="AC16PP_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481314.1"
                     /translation="MTPTDSGPATLRRFLPWLLPTALLAGHAAAADVRIGLDWNGDGL
                     IQTLPGSRVPQDAPSDSQPFVFWVNHDQDDLETGGETWPIARPDANTPAPDSSRDLED
                     FTRLHVEIDDLGSLPGDAVLTLAWASGSTAELNLFRNEDPDCSRRYLLDAEAGRRQLS
                     APHGTPIGTVGANALKIPLQTLGEPGLDGAGYCFLLEVASPGQGHLVATIADGEGVLA
                     RSEPIAAELRHIKSLYQRTTMTWPEEILAPWAYTTEAPPVPDLQWSKDPQGHEFEPGW
                     WETEDHVVWVYGWLKSGEGLREMATTQAAETIFKRLWHRGYRGRLTFFHWPTVKPRYA
                     YGLLESEYRAYKSAPALVDFVSQIPKGKRIHVTAHSLGCVLVTEAIKLGLEAEEALLQ
                     VGAIPAAAYDTRPVLTLPDMADVVTPWLPEEGGYHGYIDETPTPVYTMYNYADVTFFG
                     WNIAQKQMKPTAKRGGYRYAWEPDAPQGERARLYYKTGWFSSKYRLVDDPHEIMAFIA
                     KAKTHAIGAETRVQGHVRRVFDLARKPHDFHTGHVVGWTRPVHETTAFYNLLLDVWNI
                     AYVSELL"
BASE COUNT          520 a          996 c         1061 g          575 t
ORIGIN      
        1 tttcagcatg gaaatcgatc tgagcgtcga gggtcagatt ggcgacgata cagccatcat
       61 tgctggcggg accgaaaccg cgggcataac ggcgcctctg cccaatatct ccatgcgagg
      121 cggccaccgc tttggtgaca acttctacct ggggggtacc gccggatact tctccctcaa
      181 ggtcggggac atcgacggcg agctgatcac cgcacggggg tctcttgact ggcggccgca
      241 caagcggttc ggtctcggga tcggctacca gttcgtcagc ttcgacgtgc aggaaaagaa
      301 agagaccaag caagagagat acgacgccga cttctacggc cccatcttct tcgtctcggc
      361 cgggttctaa gagtcgcctc tcaggcagct acggacgccg cgcggcggca gcgaaacgtc
      421 taatccagcc aggtggagcc ggtcgggttc cagtgcccgc tgaacgctac gtagaaatct
      481 ccgtccatgc gcatggatac ttcgatgaaa ccgtacatcc cgtctgcgaa acggatgatg
      541 agagagcgtt ctgcgcggtc gtcccagcgg ggatcggcgg cgcggtactc gagacgactg
      601 gattccacat aaccctcctc gggcgcctga tccatgtcga tccggaaagg ctgaaatccg
      661 ccgcccggca cggtcagctc gcaccaccag tcgaagagat cgtcaggggc ggcgtcgtcc
      721 gcggttcgct cgcaggtgat gatggcttca ccggtgcccg gctccacgcc gtagggtgct
      781 acctggcgca gcgaaacctc caggggcacg ccgtcttcgg gtagccgcaa cgcgccggtc
      841 tggatgggcc tgagctcttc ctccggaggc cgccgctcga accggaactc ctccggcgcc
      901 tcccgggtgg gaaacggcaa cgtctggttc aatccttccg cgtagacgta gagcttgcgg
      961 ggcgcggtca cgggcgccca gccggggacc tcgacggtca cgtacattcc cgccccccgt
     1021 tcgcgaacgc tgaaacggcc gtcgtcgccc gtgaagactg gctctcgggc ggcgaacgcg
     1081 aagaagggaa actcgaacgg gtcggccagg cggtcgaaga cggaagtccg cacgcgcgct
     1141 cccttgagcg ggttgccctc ctcgctgagc accacgccat gaaactcgat atccgttcgg
     1201 accatcttgg acgcgtcctc gtagaaatcg tccgggtctg cgtcggcaag ggggctgttg
     1261 ctcaccgttc ttgcgaacca ggacatgtcg ctctgggacg ccacctgctc ggcccgggtc
     1321 acgcagctgc ccagcaccgc gagggctgcg cagaggagtc cggctttcag ccaatcagag
     1381 cagttcgctg acataggcga tgttccagac gtcgaggagg aggttgtaga acgccgtggt
     1441 ctcgtggacc ggtcgcgtcc agcccaccac gtggcctgta tggaagtcgt gcggctttcg
     1501 ggccaggtcg aacacccgtc ggacgtgccc ctgaacgcgg gtttccgcac cgatcgcgtg
     1561 ggttttggcc ttggcgatga aggccatgat ctcgtggggg tcgtcgacca accgatattt
     1621 ggagctgaac caccccgtct tgtagtagag ccttgcgcgc tcgccctgcg gtgcgtcggg
     1681 ttcccaggcg tagcgatacc cgccccgctt ggccgtcggc ttcatctgct tctgcgcgat
     1741 gttccagccg aagaaggtga cgtccgcgta gttgtacatc gtgtacaccg gggtcggggt
     1801 ctcgtcgatg taaccgtggt agccgccctc ctccggcagc cacggcgtca ccacgtccgc
     1861 catgtcgggc agggtgagaa ccgggcgggt gtcgtaggcg gcggccggta tggcgccgac
     1921 ctgcaggagc gcctcctcgg cttccagacc cagcttgatt gcttcggtga cgagcacgca
     1981 gccgaggctg tgggcggtga cgtggatgcg tttgcctttc gggatctggc tgacaaagtc
     2041 gaccagggcg ggggcggact tgtaggcgcg gtattcgctc tcgaggagcc cgtaggcgta
     2101 gcgcggcttc accgtgggcc agtgaaagaa cgtaagccgc ccccggtagc cgcggtgcca
     2161 gagccgcttg aaaatcgtct cggcggcctg cgtggtggcc atctcccgca atccctcgcc
     2221 gcttttgagc cagccgtaca cccagaccac gtgatcttcc gtctcccacc agccaggctc
     2281 gaactcgtgg ccctgggggt ccttgctcca ttggagatcg ggtaccggag gcgcctcagt
     2341 ggtgtacgcc catggcgcca gaatctcttc cggccaggtc atggtggtcc gctggtacag
     2401 ggacttgatg tggcgaagct ccgcggcgat gggctcggaa cgggccagga cgccttctcc
     2461 atcggcgatg gtggcgacca ggtgcccctg gccgggcgac gcgacctcca gcagaaagca
     2521 gtatccggcg ccgtccaaac ccggctcgcc gagggtttgc agcgggattt tcagggcgtt
     2581 ggcccccacg gtcccgatgg gcgtgccgtg gggtgcgctc agctgccggc ggccggcctc
     2641 ggcgtcgagc aggtagcgtc gtgagcagtc ggggtcttcg ttgcggaaga ggttcaattc
     2701 ggcggtggag ccgctcgccc aggcgagtgt cagaacggca tcgccgggca gcgatccgag
     2761 atcgtcgatc tccacatgca ggcgcgtgaa gtcttccagg tcccggctcg aatcgggagc
     2821 cggggtgttg gcgtcgggcc tggcaatggg ccaggtttct ccaccggtct ccagatcgtc
     2881 ctgatcgtgg ttgacccaga acacgaaagg ctgcgaatcc gagggggcgt cctgtggcac
     2941 gcggctgccg ggcagggtct ggatcaagcc gtcgccgttc cagtcgaggc cgatacggac
     3001 atcggccgcc gcggcgtgcc cggcaagcaa agccgtgggg agcagccagg gcaggaatcg
     3061 tctcaacgtc gccggtccag agtcggtcgg tgtcacggtt tcggtcctgc ttttcgcgtc
     3121 attcctcggt ggcactggta tccatcccgg cg
//
LOCUS       DBNHKV010000205         6869 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_453029, whole genome shotgun sequence.
ACCESSION   DBNHKV010000205 DBNHKV010000000
VERSION     DBNHKV010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6869)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_453029"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="AC16PP_06515"
     CDS             complement(<1..1083)
                     /locus_tag="AC16PP_06515"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HIR6481315.1"
                     /translation="MTIIREDDLIESVADALQFISYYHPLDFIRAMRSAYEKEESPAA
                     KAALTQVLVNSRMCAIGKRPICQDTGIVNVFLEVGMEVSWSGKRSVEEMINEGVRRAY
                     MHPDNVLRASVLTDPDGARSNSRDNTPAVIHTRIVPGDKLSVEVAAKGGGSEAKAKFA
                     MLNPSDSIVDWVLDVVPTMGAGWCPPGLLGVGIGGTPEKAMLLAKEALMEPIDIHDLK
                     ARGPANHLEELRLELFEKVNALGIGAQGLGGLTTVLDVKVKDYPTHAANKPVAVIPNC
                     TATRHVHFTLDGSGPAVFEAPDLDEWPDIAFELGDDVRRVDMDTLTPDDVRNWKAGDT
                     LLLTGKMLTGRDAAHKRLADLIERGEP"
     gene            1178..2434
                     /locus_tag="AC16PP_06520"
     CDS             1178..2434
                     /locus_tag="AC16PP_06520"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR6481316.1"
                     /translation="MRRSRILILLSTVAAGCGEPGVSPDLEHARALSRSELIVDTHID
                     LPYRLYRRHEDVAEPTSGEFDYPRALAGGLDAAFMSIYVPAAVDAEGRATEMADRLID
                     LVEGIARDAPDKFAVATCTRDVEAIRASGRIALPMGMENGGPIAGDLGKLDHFMGRGV
                     RYITLAHSKSNHISDSSYDENEPWEGLSPFGRDLVTAMNQRGMMIDVSHLSDRAFWQV
                     LELSSAPVVATHSSLRHFVPGFHRNMSDEMVAALGRNGGVVQINFGSSFVTAEARAYS
                     DAMTAAVKAFAETEEPRYDDPAVRAFAASYRAEHPYPYAELEDVLNHVDRAVALAGIE
                     HVGLGSDYDGVGDTLPVGLKDVSSYPNLIAGLANRGYDDHSISLILGGNLMRVWREVE
                     AVAAAQGFPTACRHDAPARSALSQRL"
     gene            complement(2443..3201)
                     /locus_tag="AC16PP_06525"
     CDS             complement(2443..3201)
                     /locus_tag="AC16PP_06525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HIR6481317.1"
                     /translation="MKIADVARLHRQWSYGHELGFPALLLRSEAGRDGWRLDMTARRP
                     SKGFSLIELMVVVGILAVLAAFAMPAYLDYLESANTSKVLSHYEDGTRYLRTEILRLN
                     TSVVMGKETLESIDANFTSAFWVDKLNQEGGRAPGGGPAYGASVDHGMGRVGVVVAGS
                     LLDAPPSYRLEVTRPQYGGFAQYSVMTAIISWSGGGGGTSFGSPADPTPGQPGGLDGS
                     GPGQGAGSADNPGQGKDQGQDKGKGQGQGQGQNV"
     gene            complement(3290..4441)
                     /gene="argE"
                     /locus_tag="AC16PP_06530"
     CDS             complement(3290..4441)
                     /gene="argE"
                     /locus_tag="AC16PP_06530"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF005710.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HIR6481318.1"
                     /translation="MARRFTPREMLEKLIAFPTVSRDSNLELIEFVANYLDNHRVQAR
                     LVASGDGRKANLYATVGPEIEGGVVLSGHTDVVPVDGQNWDSDPFLLHEVDGRLVGRG
                     TCDMKGFLAIALALVPDMGALRYPIHLAFSYDEEVGCLGAPDLIREMTANVPRPKAVI
                     VGEPTSMQVVGAHKSMFIFDTVVRGHEAHSSQQHRGVPAVMVAGRLIGWLAERQRQNA
                     RVADPDSDFVPGYTTLHCGTVRGGTAHNITAAECRFATDIRSLPGEPAESYFREFEQF
                     ARQTLEPEMRAVHPGSGIDFEIRAQVPAFQATGDDPAVALASQLTGQNGLESVPYGAE
                     AGQFQEAGHSVVMCGPGSIDQAHQPNEFLTLEQLQAGATFARRLIEQLS"
     gene            complement(4441..6165)
                     /locus_tag="AC16PP_06535"
     CDS             complement(4441..6165)
                     /locus_tag="AC16PP_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481319.1"
                     /translation="MSGQTNSGGGEKVPLVIGVTGHRDLLAEELPVIRRRVQDLFAEL
                     QRQFPDLPILVMTPLAEGADRLVAEVARDMGMPVEVLMPMPRELYQADFSGESLVEFD
                     EMLRLGEIVELPLVPGNTEKTVAAGGRPRDLQYAQLGAYLAAHSHVLLAIWDGKPPEA
                     VGGTAHVVQFHQHDVIELLAEGQHRSPIDFAEDESDLVFHIACSRLADGAPREPLQPG
                     QAEWLTRDDVQPRTPQMPPRYRVVFERLVEFNRDLAEPIPAGSLDPLVAGDDADHCGP
                     GAEDIERLYMVADFLARHYKKYRRRLQTATYALAVCAIMSFIVWADLPDQEIMIYPYL
                     GFIGLVIVLVYLERRKGWQRRSLEYRVLAEGLRVQFWWSMAGVVMENPSRFSHDSFLR
                     QQDLELGWIRNVMRFAGRRADANIGNEDGADTDLVARVWVADQKAYYQSKSQERNRGS
                     QLTSALVFATFGVGVTVAIIFAVFQAEIAAPWSNMLVALMGLLPFLAAVRQSYANSTA
                     EREDFVQFGYMYRIFFNADRLLNSARTVDGRRDILRALGEAALEEHGQWMLRQRERPA
                     AGRVLQGG"
     gene            6220..>6869
                     /locus_tag="AC16PP_06540"
     CDS             6220..>6869
                     /locus_tag="AC16PP_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481320.1"
                     /translation="MGQTDSTSRSDVIDQLVASYLKIPLKIYWRETRGLPFPDTFIDA
                     RLEFAGMTTSWLRLDSVIWRAGEVRFVPGFPARLYVRSPSVEFVVGQAELDRWQSRFS
                     LPYRLELTESGITVHTELAGFPLAEFETGLTVVDGWFVLEPKRATVLGVPGYVSSLFR
                     GYLPVPPLSGEIRLAGIEHAAGILRVRFDIDDFDEEVTPGMLSRLQKRLFSFNSPLG"
BASE COUNT         1232 a         2316 c         2072 g         1249 t
ORIGIN      
        1 aggctcgcca cgctcgatga ggtccgccag ccgcttgtgg gcggcgtccc ggcccgtcag
       61 catcttgccg gtgagcagca gcgtatctcc cgccttccag ttgcgcacat cgtccggcgt
      121 cagcgtatcc atgtcgacgc gccgcacgtc gtcgcccagc tcgaaagcga tgtccggcca
      181 ctcgtccaga tccggcgcct cgaagacagc gggcccggat ccgtccaggg tgaaatgcac
      241 gtgacgcgtc gccgtgcagt tggggatcac ggccaccggc ttgttggccg catgggtcgg
      301 gtagtccttc accttgacgt cgagtacggt ggtcagccca ccgagcccct gagccccgat
      361 accaagcgcg ttgaccttct cgaagagctc gagacgcagc tcttctaggt ggttggcggg
      421 accgcgggcc ttgagatcgt gaatgtcgat gggctccatg agcgcttcct tggcgagcag
      481 catggccttt tccggcgtcc cgccgatgcc gaccccgagc agccccggcg ggcaccagcc
      541 ggcgcccatg gtcggcacca cgtccagcac ccagtcgacg atggagtcag acgggttgag
      601 catggcgaac ttcgccttgg cctcgctgcc accacccttc gcggccacct ccacggaaag
      661 cttgtcaccg ggaacgatcc gggtgtggat caccgccggc gtgttgtccc tggaattgct
      721 gcgcgctccg tccgggtcgg tcagcaccga cgcgcgcagc acgttgtccg ggtgcatgta
      781 ggcgcgacgg actccctcgt tgatcatctc ctcgacgctt cgcttgcccg accacgagac
      841 ctccatgccg acctcgagga acacgttgac gatgcccgtg tcctggcaga tcggccgctt
      901 gccgatggca cacatgcgcg agttcaccag aacctgggtc agcgccgcct tggccgccgg
      961 agactcttct ttctcatatg cgctgcgcat ggcgcggatg aagtccagcg gatggtagta
     1021 ggagatgaac tgcagcgcgt cggcgacgct ttcaatgagg tcatcctcac gaatgatggt
     1081 catcttgatt cctgcccggt tgcagagcgc attttaactc agcttgtgct gcgcggttcg
     1141 ggcgtgtgaa cataatccct ccgcggagat ggctcacatg cgacgctcga gaattctgat
     1201 tctattgtcc accgtcgccg ccggctgcgg agagcccgga gtgagcccgg acctggagca
     1261 cgcgcgagcc ctctcccgca gcgagctcat cgtagatacc cacatcgacc tgccctaccg
     1321 gctttaccgg cgccacgaag acgtcgccga gcccacgagc ggcgagttcg attacccgag
     1381 ggccctcgcc ggaggactgg acgcggcgtt catgtccatc tatgtccccg ccgccgtcga
     1441 tgccgaagga cgcgcaaccg agatggccga tcgtctcatc gacctggtgg agggcatcgc
     1501 ccgcgacgcg ccggacaagt tcgccgtggc aacctgtacc cgagacgtgg aggccattcg
     1561 cgcgtcggga cgcatcgctc tgccaatggg catggagaac ggcggcccca ttgccggcga
     1621 tctgggcaag ctcgatcact tcatgggccg cggcgtccgc tacatcacgc tggcgcactc
     1681 gaagtcgaat cacatctcgg attcctcgta cgacgagaac gagccctggg agggcctgtc
     1741 gcccttcggc agagacctgg taacggcgat gaaccagcgc ggcatgatga tcgatgtgtc
     1801 ccacctcagt gaccgcgctt tctggcaggt gctggagctc tcgagcgcgc cggtggtggc
     1861 aacgcactcc tctctgcgcc atttcgtgcc gggctttcac cgcaacatga gtgacgagat
     1921 ggtggccgcg ctgggacgca acggcggcgt cgttcagatc aatttcggca gcagcttcgt
     1981 caccgccgaa gcccgggcgt actcggacgc catgaccgcg gccgtgaaag cattcgccga
     2041 aaccgaggag ccgcgctacg acgatccggc cgtgcgggcg ttcgccgcaa gctaccgcgc
     2101 cgagcacccg tatccctacg cggagctcga agacgtcctg aatcacgtcg accgcgccgt
     2161 tgcgctggcc ggcatcgagc acgtcggcct cggttccgac tacgatggcg tgggcgacac
     2221 cctgccggtg ggtctgaagg acgtcagctc gtacccgaac ctgattgccg gcctggccaa
     2281 ccgggggtat gacgaccaca gcatctccct gattctcggc ggcaacctga tgcgcgtgtg
     2341 gagggaagtg gaggccgttg ccgccgccca gggatttccg acagcctgcc gccacgacgc
     2401 tccggcgaga tcggcgctca gccagcggct gtaaaagcca ccctaaacgt tttggccctg
     2461 accctggcct tgacctttgc ctttgtcttg cccctgatct ttgccttggc caggattgtc
     2521 tgcgcttcct gcgccctggc ccgggccgga accatccaag ccgccaggct ggccaggggt
     2581 gggatcagcc ggcgagccaa acgatgttcc cccgccgcca ccactccagg aaatgatcgc
     2641 ggtcatcacc gagtactgtg cgaacccgcc gtactgggga cgagtcacct cgagtcgata
     2701 ggatggcgga gcgtccaaca aactaccagc gaccaccaca cccacacggc ccatgccgtg
     2761 atcgacggag gctccgtaag cagggccccc tccgggagcg cgccctcctt cctgattcag
     2821 cttgtcgacc cagaatgcag acgtgaagtt cgcgtcaatg gattcgaggg tttcttttcc
     2881 catgacgacc gacgtgttca gcctgagaat ttccgttctg aggtagcgcg tgccatcctc
     2941 gtaatgagaa agaaccttgc tggtgttggc gctctccaag tagtcaagat aagcgggcat
     3001 ggcgaaggct gccaaaacgg cgagaatacc caccaccacc atgagttcta tcagcgaaaa
     3061 gcctttcgag ggccgccgcg ccgtcatatc gagtcgccat ccatctctac cggcctctga
     3121 tcggagcagg agcgccggga aacccaattc atgtccgtat gaccactgac gatgcagcct
     3181 cgcaacgtcg gcaatcttca cctatgcgct ggcaaatgct acacaaatca gttcacaaat
     3241 cgattgacag acgtctcacg gctatcgtgc gtcggggccg aatttctaat caagaaagct
     3301 gctcgatcaa gcgtcgcgca aacgtcgcgc cggcctgcag ctgctcgagg gtgaggaact
     3361 cgttgggttg atgggcctga tcaatgctgc cggggccaca catgaccacc gagtgcccgg
     3421 cttcctgaaa ctgccccgcc tcggctccgt acggcacgct ttccagccca ttctgcccgg
     3481 tcagctggct ggcaagtgcc accgccgggt cgtcgcccgt tgcctgaaag gctgggacct
     3541 gagcccggat ctcgaagtcg atcccgctgc ccggatgcac cgcgcgcatt tccggctcga
     3601 gcgtctgcct tgcgaactgc tcgaactccc gaaaatacga ttcggcgggc tcgccgggaa
     3661 gcgagcggat gtccgtcgcg aaacggcatt cggcggcggt gatgttgtgg gcggtccctc
     3721 cccgcaccgt gccgcaatgc agggtcgtgt agcccggcac gaaatcggaa tccgggtccg
     3781 ctacccgggc gttctggcgc tgccgctcgg cgagccagcc gatcagccga ccggcaacca
     3841 tgaccgcggg gacccctctg tgctgctggc tggaatgcgc ctcgtggcca cgaacgacgg
     3901 tatcgaagat gaacatgctc ttgtgggccc ccaccacctg catgctggtg ggctccccca
     3961 cgatcaccgc cttggggcga ggcacgttgg ccgtcatctc ccgaatgaga tcgggcgcgc
     4021 ccaggcaacc cacttcctcg tcgtaagaga aggccaggtg aatcggatag cgaagcgccc
     4081 ccatgtccgg gacgagagca agagcaatgg caagaaaacc cttcatgtcg caggtaccgc
     4141 ggcccaccag cctgccgtcg acctcgtgaa gaaggaacgg gtccgagtcc cagttctgcc
     4201 cgtccacggg caccacgtcc gtatgccctg acagcacgac gccaccttcg atctcaggcc
     4261 cgaccgtcgc atacagattg gctttgcgtc catcgccgct ggcgacgagt ctggcctgca
     4321 cgcggtgatt gtcgagatag ttggcgacaa actctatcag ctcgaggttg gagtctctcg
     4381 agaccgtggg aaaggcgatc agcttttcga gcatttcccg cggtgtgaat cgtcgcgcca
     4441 ttacccgccc tgcagaaccc ggcccgcggc cggccgctca cgctggcgga gcatccattg
     4501 accatgctct tcgagcgccg cctccccgag cgcccggagg atgtcgcggc ggccgtcgac
     4561 cgtgcgggca gaattcaaga ggcggtccgc attgaaaaat atccggtaca tgtagccaaa
     4621 ctgaacgaag tcctctcgct ccgcggtgct gttggcatag ctctgccgta ccgcagcgag
     4681 aaagggtaac aatcccatca gggccaccag catgttggac cagggcgcgg caatctccgc
     4741 ctgaaagacc gcaaagatga tcgccactgt cacgccgact ccgaatgtgg cgaagacgag
     4801 ggccgaggtc aactgactgc ctctgttgcg ctcctgggac ttgctctgat agtacgcctt
     4861 ctggtccgcg acccagaccc gggccaccag gtcggtgtcg gccccgtcct cgttgccgat
     4921 gttggcgtcc gcccggcgac ccgcgaatct catcacgttg cggatccagc ccagctccag
     4981 atcctgctgg cgcagaaaac tgtcgtggct gaagcggctg gggttctcca tgaccacccc
     5041 cgccatgctc caccagaact gcacgcgaag gccctccgcg agcactcgat attccagcga
     5101 tcggcgttgc cagcctttgc gtcgctccag atagaccaga acgatgacga gcccaatgaa
     5161 accgaggtag gggtagatca tgatctcctg gtccggcaga tcggcccaca cgatgaaact
     5221 catgatggcg cacaccgcca gcgcgtaggt ggcggtctgc aaccgtcgcc gatatttctt
     5281 gtagtgccga gccagaaagt ccgccaccat gtacagacgc tcgatgtcct cggcgccggg
     5341 cccgcaatga tcggcatcgt cgccggcgac cagaggatcc agcgagcctg ccgggatcgg
     5401 ctcagccagg tccctgttga actccaccag gcgctcgaat accacccgat acctcggcgg
     5461 catctgcggc gtccgcggtt gcacgtcgtc acgcgtgagc cactccgcct gaccgggttg
     5521 cagcggctct cgcggcgccc cgtcggccag acgcgagcat gctatgtgaa acacgagatc
     5581 gctctcgtct tccgcgaaat cgatgggcga gcggtgctgt ccttccgcca gaagctcgat
     5641 gacgtcgtgc tgatgaaact gcaccacgtg cgccgtgccc cctaccgctt cgggcggctt
     5701 gccatcccag atggccagca gaacatgact gtgagccgcg aggtatgctc ccagctgagc
     5761 atattgcagg tcacggggcc gccccccggc cgccaccgtc ttctcagtgt tgcccggcac
     5821 gaggggcaac tccacaattt ctcccagcct cagcatctcg tcgaactcga ctagcgattc
     5881 gcccgagaaa tccgcctggt aaagctctct gggcatgggc atgagaacct cgaccggcat
     5941 gcccatgtcc ctggccacct cggcaacgag ccggtccgcc ccctcggcta gaggcgtcat
     6001 gaccaggatc ggcagatcgg ggaactgccg ctgcagctcg gcaaacagat cctggacccg
     6061 ccgccggatc accggcagct cctcagcgag aagatcccga tgccccgtca cgccgatgac
     6121 gagcggcacc ttttccccac caccgctgtt ggtctggccg gacatggcgg aaacttaacc
     6181 cgtatttgtt cccaggcaaa gcctgggtaa actccgctca tgggccagac cgattcgacg
     6241 agccgttcag acgtaatcga ccagctggtc gcttcctacc ttaagattcc gctgaagatc
     6301 tactggcgcg agacccgcgg gctccctttc cccgacacct tcatcgacgc acgactcgaa
     6361 ttcgcgggca tgacgacttc ctggctgcgc ctggacagcg tgatctggcg tgccggtgaa
     6421 gtgcgattcg tcccggggtt tccggctcgg ctctacgtgc ggtcgcccag cgtggagttc
     6481 gtcgtgggcc aggcagagct cgaccgctgg cagtcgcggt tctcgctgcc gtatcggctc
     6541 gagctcacgg agtccgggat caccgtgcat acggagctgg ccggatttcc tctagccgag
     6601 ttcgagacgg gcctcacggt cgtggacggg tggttcgttc tggaacccaa acgagccacc
     6661 gtgctcggtg tgcccggcta cgtgtcatcc cttttccgcg gctatctgcc ggtcccgccc
     6721 ctgtccggcg agatacgtct agccggcatc gagcacgccg ccggcattct gcgcgtgcga
     6781 ttcgacatcg acgacttcga cgaggaggtc accccgggca tgctgtcccg gctccagaaa
     6841 cggctgttct ccttcaactc gccactggg
//
LOCUS       DBNHKV010000206         2613 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_666787, whole genome shotgun sequence.
ACCESSION   DBNHKV010000206 DBNHKV010000000
VERSION     DBNHKV010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_666787"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /locus_tag="AC16PP_06545"
     CDS             complement(<1..1198)
                     /locus_tag="AC16PP_06545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019395839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6481321.1"
                     /translation="MERESMDFDVVIVGGGPSGLAAACRLMQLAESSGQELSVCLVEK
                     GSEIGAHILSGAVFEPTALNELFPDWEERGAPLDNPVTLDEVYYLVNEENRLKMPGFA
                     VPKVTHNHGNHAISLGNLCRWLGEQAENMGVNVFPGFAAAEILYGEDGAVAGVATGDM
                     GVGADGEHKATYAPGYELRGKYTIFAEGCRGHLGKQLMAKFDLQRDSATQHYAIGFKE
                     LWDVTPENHVPGKVVHTMGWPGRHLKGMFTGSYLYHLPNQQVSLGLIVDLSYSNPHVS
                     PFDEFQRWKHHPLIAETLSGGKRVSYGARAIVKGGLSALPKLSVPGALLVGDDAGFLN
                     MTKIKGSHTAMKSGMLSAEAVFESLSGGSAGGEDLSSFQEKFDSSWLHEELSAARNSQ
                     QAIHKFG"
     gene            1460..2212
                     /locus_tag="AC16PP_06550"
     CDS             1460..2212
                     /locus_tag="AC16PP_06550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR6481322.1"
                     /translation="MKVLVPVKRVVDYNVKIRVKPDNTGVDLNNVKMSLNPFCEISVE
                     EAVRLKEAGTATEVVAVAVGGTVCQEQLRTCLALGADRAVLIETDQEPQPLGIAKALK
                     ALQERENADIVIFGKQSIDGDNAQTGQMFAALTGMPQGTFASELTVADGKAQIVREVD
                     GGLQTVSVTLPCVVTTDLRLNEPRYASLPNIMKAKKKPLDVVTPDELGVNVAPTVETL
                     SVKPPAERQAGIKVGSVEELVDKLKNEAKVIS"
     gene            2209..>2613
                     /locus_tag="AC16PP_06555"
     CDS             2209..>2613
                     /locus_tag="AC16PP_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR6481323.1"
                     /translation="MSVLVVADHDNAELKPVTLVVVAAATAIGGDIDILVAGEGCGAV
                     AETAAKIPGVNKVLLADNAAYGHQLAENVAPLVAEIGAGYSHILTAHTTVGKNFLPRV
                     AAVLDVAQISDIQGVVGPDTFERPIYAGNAVAT"
BASE COUNT          485 a          812 c          845 g          471 t
ORIGIN      
        1 tgccgaactt gtggatggcc tgctgggaat tccgcgccgc tgaaagctcc tcgtgcagcc
       61 aggacgagtc gaacttctcc tggaagctgg acaggtcctc gccgcccgcg gaaccgccgg
      121 aaagggactc gaagacggct tcggccgaga gcatgcccga tttcatggcg gtgtggctgc
      181 ccttgatctt ggtcatgttg agaaagccgg catcgtcacc caccagcagc gcgccgggca
      241 ccgacagctt gggcagcgcg gacaggccgc ccttgacgat ggcccgggca ccgtaggaga
      301 cgcgcttgcc cccgctgagg gtctcggcga tgagcggatg gtgcttccag cgctggaact
      361 cgtcgaacgg gctcacgtgg ggattgctgt aggacaggtc cacgatcagc ccgagagaca
      421 cctgctggtt gggcaggtgg tagaggtagg acccggtgaa catgcctttc aagtgcctgc
      481 caggccagcc catggtgtgc accaccttgc ccggcacgtg attctcgggg gtcacgtccc
      541 atagttcctt gaagccgatg gcgtagtgct gggtggccga atcccgctgc agatcgaact
      601 tggccatcag ctgcttgccg agatggccgc ggcagccctc ggcgaagatg gtgtacttgc
      661 ccctgagctc gtatccgggc gcgtaagttg ccttgtgttc gccgtccgcg ccgaccccca
      721 tgtcccccgt ggcgacgccg gccacggccc cgtcttcccc atagaggatc tcggcggcgg
      781 caaatccggg aaagacgttc acgcccatgt tctcggcctg ctccccgagc cagcggcaga
      841 ggttgcccag actgatcgcg tggttgccgt gattgtgggt gactttgggc acggcgaagc
      901 ccggcatctt caggcggttt tcctcgttga cgagatagta gacctcgtca agggtcaccg
      961 ggttgtccag cggcgcgcca cgctcctccc agtcgggaaa caactcgttc aacgcggtgg
     1021 gctcgaaaac ggcgccggaa agaatgtgcg ccccgatctc cgagcctttc tccaccaggc
     1081 agacgctcag ctcctgaccc gatgattcgg ccagctgcat cagccggcag gcagctgcca
     1141 ggccggaggg accaccgccc acgatgacga cgtcgaaatc catggactct ctttccacga
     1201 tgactctcca ctgctttgat ttgcgggttt gcgtcgcagc ttcaaaggcc ccgaccgggc
     1261 gggctgctgg gctcaaatat tagctcaaac attatagggg agggccttca cccgcgcacc
     1321 ggttctgcgc tgccgatcct cgggccccct tcgtcttgac ctcggtgagg acccaaggga
     1381 gaatacgcgc ccctccgggt agccgtcgaa acgtcggcac ctgctgttct gtttaacttg
     1441 ttagccgttc gaggaattca tgaaggtact ggttccggta aaacgcgtgg tcgactacaa
     1501 cgtcaagatc cgggtcaagc ccgacaacac tggcgtcgac ctgaacaacg tcaagatgtc
     1561 gttgaaccca ttctgcgaga tttccgtgga agaagccgtc cgcctcaagg aagccggaac
     1621 ggctaccgag gtggtcgccg tggcggtcgg tggcaccgtt tgtcaggagc agctcagaac
     1681 ctgcctggcg ctcggggccg atcgtgcggt gctcatcgag acggatcagg agccgcagcc
     1741 tcttggcatt gccaaggcgc tcaaggcgct gcaggagcgg gaaaacgccg acatcgtgat
     1801 cttcggcaag cagagcatcg atggtgacaa cgcccagacc ggccagatgt tcgccgctct
     1861 gaccgggatg cctcagggca cgttcgcctc ggagctcacc gtagccgatg gcaaagcgca
     1921 gatcgtgcgg gaggtggatg gcggtctgca gacggtctcc gtcacgctgc cctgcgtggt
     1981 caccacggac ctgcggctga acgagccgcg ctacgcgtct ctccccaaca tcatgaaggc
     2041 caagaagaaa cctctggacg tcgtcacgcc ggacgagctg ggggtgaatg ttgctcccac
     2101 cgtggagacg ctttccgtca aaccgccggc cgaacggcag gcgggtatca aggtggggag
     2161 cgtcgaggag ctggtcgaca agctgaagaa cgaggcaaag gtaatctcat gagcgtattg
     2221 gtagttgcag accacgacaa cgcggaactc aagccggtca ccctggtggt ggtggcggcc
     2281 gcgacggcca tcggcggcga catcgacatc ctggtcgcag gtgaaggctg cggcgcggtg
     2341 gctgaaaccg ccgccaagat tcccggggtc aacaaggtgc tcctggcaga caatgctgcc
     2401 tacgggcatc agcttgcgga gaacgtcgcg ccgctggtgg cggaaattgg cgccgggtac
     2461 tcccacatcc tcactgccca taccacggtc ggcaagaact ttctgccccg ggtggcagcc
     2521 gtgctcgacg tggcgcagat atccgacatc cagggtgtgg tcgggccgga taccttcgag
     2581 cggccgatct acgccggcaa cgctgtggcg acg
//
LOCUS       DBNHKV010000207         3121 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1730457, whole genome shotgun sequence.
ACCESSION   DBNHKV010000207 DBNHKV010000000
VERSION     DBNHKV010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1730457"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="AC16PP_06560"
     CDS             <1..684
                     /locus_tag="AC16PP_06560"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR6481324.1"
                     /translation="YGSGCYRGLGQDGMIRKAERYAADGFSAIKMQVAHMATHDQDVE
                     NVAAMRAALGADIEIMVDVNQGWTAAEAIEVGSRLDESGIYWLEEPVIAHDFAGYRQV
                     ARALKTRVVGGENHFTHHDLQPFFDDPCVPILQPDVMRGGLTELRRIAAMAEPLGISI
                     APHLFPELMTHLLASIPNGSWLEYMGWHDELWAQPVVPSGGLTRPPDRPGHGLAFRAD
                     VLAEYRVAG"
     gene            complement(681..1907)
                     /locus_tag="AC16PP_06565"
     CDS             complement(681..1907)
                     /locus_tag="AC16PP_06565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6481325.1"
                     /translation="MHDDDKTNDDLGLGRNPTVTLLLAFFQVFLVIMPVAVPFFQSKG
                     LSMQEVFSLQALFALVVLLAEVPSGYVADIFGRKRTLIVGAAFCGLGHTLLLGADGFW
                     TLALFEISLGIGSSLISGADIALLYDTEVALGRGERQQRQVVGRLYSMRTFSEAVAGV
                     VCSVVLLWSMDLAVYVQALIGWLPLVFAFALVEPPGERMERSGHLVNMARICRYLVSH
                     SAVLRLTFLALCIWALTTFYAVWLLQKIWEAQGLGLIHFGYLWGFLSLVAALAGRWAY
                     RAEEMLGTTGVLVFIGLAPAAGYLCLDLFGVVGSVLAGLTFWISRGLGMVILRDALNR
                     RIPGEFRATANSLASFGFRGAFVLTGPLVGYAFDLWGMSVTLSLLALATLVIFAGLVL
                     PLVAAARSQRLEAQAT"
     gene            complement(2337..>3121)
                     /locus_tag="AC16PP_06570"
     CDS             complement(2337..>3121)
                     /locus_tag="AC16PP_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481326.1"
                     /translation="MPPYFLCMVTLQRLRTEESLAEMADLLEPMSAARVQGCIAGIHP
                     AGWFLVVLGAVFGGYQNEEFIRRVGASGTLNAMDVAFVLANCFVWAAIAFVLSWRLPI
                     SLALSRLGAQMKVDVYRLDRLRPLARIATLDILVVAGAMALMPLQSLDAEFRIYNYNY
                     GIIVGVPSAIVLFVLPLWGVRGNIGDRKADRVAVLQAKLDVVDRNDVAAVEALSAHIE
                     RLRGLSNWPVDLRLATRIFAYVIIPPLAWVGAALVEILIDRL"
BASE COUNT          636 a         1066 c          968 g          451 t
ORIGIN      
        1 tacgggagcg gttgctaccg cggcctcggc caggacggga tgatccgcaa ggccgagcgt
       61 tacgcagccg acggcttttc cgccatcaag atgcaggtgg cccacatggc cacgcacgat
      121 caggacgtgg agaacgtggc cgccatgcgc gcagcgcttg gggcggacat tgaaatcatg
      181 gtggacgtca accagggctg gacggcggcg gaagccatcg aggtcggatc gcggctggac
      241 gaatcgggca tctactggct tgaagagccg gtgatcgccc acgatttcgc gggctaccgg
      301 caggtcgccc gggccctgaa gacacgggtg gtcggtggcg agaaccactt cacccaccac
      361 gatcttcagc ccttcttcga cgatccctgc gtgcccatcc tgcagcccga cgtcatgcgc
      421 ggcgggctga cggagctcag gcggatcgcg gccatggcag agcccctcgg catcagcatc
      481 gcgccccacc tgttccccga gctcatgacc caccttctcg ccagcattcc caacgggagc
      541 tggctcgagt acatgggctg gcacgacgag ctgtgggcgc agccggtggt cccgtccggc
      601 gggcttacgc gcccgccgga caggcccgga cacgggctgg cgttcagagc cgacgtcctc
      661 gccgagtacc gcgtcgccgg ctaggtagcc tgggcttcca gcctctggct gcgggcggcg
      721 gcaaccagcg gcagcaccag cccggcgaat atgaccagcg tcgccagcgc gagcaggctc
      781 agcgtgacgc tcatgcccca gaggtcgaag gcgtagccca ccaggggccc ggtcaggacg
      841 aaggcgcccc ggaagccgaa gctcgccagc gagttggccg tcgcccgaaa ctccccggga
      901 atccgccgat tgagcgcatc ccgcaggatc accatcccga gcccccgact gatccagaac
      961 gtcagcccgg caagaacgct gcccaccact ccgaacaggt ctaggcaaag gtacccggcg
     1021 gcgggcgcca ggccgatgaa gaccagcacg cccgtggtgc cgagcatctc ctcggcccgg
     1081 taagcccacc gtccggccag agcggcgacc aggctcagga acccccagag gtaaccgaag
     1141 tgaatcagtc ccaggccctg tgcctcccag atcttctgca gcagccagac ggcgtagaac
     1201 gtcgtcagcg cccagataca cagcgccagg aaggtcagcc ggagcacggc gctgtggctc
     1261 accagatagc gacagatacg ggccatgttc accaggtgcc ccgagcgctc catacgctcg
     1321 cccgggggct cgaccagagc gaaggcgaac accaggggta gccagccgat cagcgcctga
     1381 acgtaaacgg ccaggtccat ggaccacagc agcacaacgc tgcagacgac gccggccact
     1441 gcttccgaga acgtccgcat cgagtacagc cgaccgacca cctgccgttg ctgacgctcg
     1501 ccgcgcccca gcgccacttc cgtgtcgtag agcaacgcga tgtcggcgcc cgagatcagg
     1561 ctgctgccga tgcccagcga tatctcgaac agcgccagcg tccagaaccc gtccgcgccg
     1621 aggagcagcg tgtggcccag accgcagaac gccgcgccga cgatgagcgt gcgcttgcga
     1681 ccgaagatgt cggcgacgta gccggaaggg acctcggcca ggagcaccac cagggcaaac
     1741 agagcctgca gcgagaacac ctcctgcatg ctcagcccct tgctctgaaa gaacggcaca
     1801 gccaccggca tgatgaccag aaacacctgg aagaatgcca gcagcagcgt caccgtcggg
     1861 ttgcgaccca acccgagatc gtcgttcgtc ttgtcatcgt cgtgcatctt catccctccg
     1921 accgttcgcc gggacccgcc acggacgccg gaatccggcg cccagaaaag aaaaaccccg
     1981 aaggcttgcg gctcttcggg gtttttttcg gaactcgctg ctgcgtttcc taaagcatca
     2041 tccgctcgag ccgtgccctc ctcgcgcaat acgcaccatg tggtccgcac acagggactg
     2101 cgcagacaca cggccacccg cgaaggtggt agctggtttg tttcgatacg cgttcatgat
     2161 ctcgagcttc tctgatccgg ctcacgccga aaaaacaggg cgcgcatgtt gcgccgggcg
     2221 ttctctggtg tcaacggaag tcagcaaaaa aagggggaat ctgtgacccc tttcagcaag
     2281 ccagcctcca gacagcttcg agcatcgggg tccgatcgct ggggttgggc ttgctgctag
     2341 aggcgatcga tcagaatctc gaccagcgcc gcgcccaccc atgccaacgg cgggatgatc
     2401 acgtacgcga agatgcgggt ggcaaggcgc agatcgaccg gccagttgga aagaccccgc
     2461 aaacgttcaa tgtgtgcgct cagggcttct acggcggcta cgtcattgcg gtccaccaca
     2521 tccagcttcg cctgcagcac ggcaacccga tcggccttgc ggtcgccgat gttcccgcgc
     2581 accccccaca ggggcagaac gaacagcacg atggcagacg gcacacccac gatgatcccg
     2641 tagttgtagt tatatatccg gaactcggca tccagggact gcagcggcat caacgccatg
     2701 gcgccggcaa ccaccaggat gtccagcgtg gcgattcggg ccagcggccg cagccgatcc
     2761 agccggtaga cgtcaacctt catctgcgcg cccagtcgcg acagcgccag gctgatcggc
     2821 aggcgccagc tgagaacgaa cgcgatggcg gcccagacga aacagttggc aagcacgaag
     2881 gccacgtcca tggcgttgag cgtgccactt gcgccgactc tgcggatgaa ctcctcattc
     2941 tggtagccac cgaacaccgc cccaaggacg acgaggaacc aacccgcagg atgaatgccc
     3001 gcgatgcatc cttggacccg ggcggcgctc atcggctcga gcaggtcggc catctcggca
     3061 agggattcct ccgtcctgag ccgctgaagg gtgaccatgc agaggaaata gggcggcatt
     3121 a
//
LOCUS       DBNHKV010000208        11155 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_381934, whole genome shotgun sequence.
ACCESSION   DBNHKV010000208 DBNHKV010000000
VERSION     DBNHKV010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11155)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11155)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11155
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_381934"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..480)
                     /locus_tag="AC16PP_06575"
     CDS             complement(31..480)
                     /locus_tag="AC16PP_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481327.1"
                     /translation="MTMPDASEIEALVGHEFPGGKYTVAHWENFLLTECTGAEQLPEG
                     MVHPVVMFHMPILGAGTSIGEMFALGQAESDYSIGIESYDWEFFGTLEEDVEYSISAR
                     VTEADRRRSGDRVYDRIQFQFDVADPGGTVLARSTITWTYGRSGDAS"
     gene            602..1153
                     /locus_tag="AC16PP_06580"
     CDS             602..1153
                     /locus_tag="AC16PP_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6481328.1"
                     /translation="MSKIDPYASWLPLEARAAAETNPRHKQLLLEVRDHMEHEIKGAL
                     EPLMATLTAEPVYHFWGNEPSVLNGRDAVRDFYKGMIAMGGQQFQVVVDRIVVDDGAV
                     VTEGQVKQVYKGAALKAMGMDAVGGERVADDDLFMTTAQLVTVWPADAVGKLIGEDIY
                     FGHDPFRNVERIGREDLPDYYQI"
     gene            1153..2862
                     /gene="ggt"
                     /locus_tag="AC16PP_06585"
     CDS             1153..2862
                     /gene="ggt"
                     /locus_tag="AC16PP_06585"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione gamma-glutamate
                     hydrolase [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR6481329.1"
                     /translation="MIPKTKTAYYGIGWLLMLLALTGQTALAASAAHRAQHGMVVTQS
                     AIASDVGARVLRAGGNAIDAAVATAFALAVTYPEAGNIGGGGFLVYQPQEGGADAYDF
                     RETAPTGSHPQMWLDEDGEYSPARQYVGPVAAGVPGTVAGLHKAWRDHGSLPWAELVA
                     PAIGLARDGFAIPEDLARSIAATLPQMEAYPASLAQFSRDGAPMAPGELLVQRDLART
                     LELIAADGPDAFYRGPIAEQIVALMASTGGLITAGDLASYRAVKRKPIRGTYRGYEIV
                     SMPPPSSGGVALVQTLNLLEGYDIAGEGFGSARTLHLITEALRRTFADRARYLADPDF
                     VKDMPVRRLTSKSYAAELRETISLNRAGTSSPETFEWPTASNDTAHFSVVDAQRNAVS
                     LTYTLNGRYGNQMVVPGAGFLLNNEMGDFNPVPGLTDERGRIGTTPNLAAPGKRMLSS
                     MSPTIVLKDGELFMVTGARGGRSIISTVLQTIMNVVDHGMNAQAAVDARRIHHQWLPD
                     RLRYEPQSFSRDTLNALRWLGHEIEQGGASWSNAEVIVVANDGWLEGGDDRRRSRGSV
                     AGY"
     gene            2904..3758
                     /locus_tag="AC16PP_06590"
     CDS             2904..3758
                     /locus_tag="AC16PP_06590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6481330.1"
                     /translation="MKIGLSNFPTEYSISAVALARAAEERGFESLWVVEHTHIPASRR
                     TPYPIGGDLPSIYWESYEPFTFLAQAAAVTTDLRVGTGICLVPEHHPIALAKRVASLD
                     ALSGGRFLFGVGAGWNAEELENHGVAFGDRWKVLREHVLAMKACWTEKDAEYHGRFVD
                     FDPVWVEPKPVQKPHPPIYIGASSTWAMERIVEYGDGWLPVPVPEFDERLALLNRLCE
                     ERGRDRSEIDVCLMTVVDSADTLAGIRDKGVNRVILSLPTLGEDEALRVLDGYANVVE
                     WAKEIGDG"
     gene            complement(3772..4374)
                     /locus_tag="AC16PP_06595"
     CDS             complement(3772..4374)
                     /locus_tag="AC16PP_06595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6481331.1"
                     /translation="MNARSPGEPVADQDRKKWDQRYAAGAYADRPHPSAILADWLPRL
                     SAGDALDIACGAGRNALHMARAGFRVDAVDVSAEALARAASAAAEAGLEVNWMQRDLD
                     TGLPVEGPYDLIVMIRYVDESLLRALPALLRPGGVLIVEEHLTTEEPVIGPRDARFRV
                     MPGALRAAVPDLEVLEATDTVTDDPDGRRVALARLVARKP"
     gene            4568..4930
                     /locus_tag="AC16PP_06600"
     CDS             4568..4930
                     /locus_tag="AC16PP_06600"
                     /inference="COORDINATES: protein motif:HMM:NF039701.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HIR6481332.1"
                     /translation="MEDILVPISLFLVIGAVISLSFYFRYRTRQEIQSTVRAAIERGQ
                     ELTPEVLDGLSDALNSRHGDLRRGIISLAIGIALFLFAGLLGEPDAEGPLMAISAFPL
                     LLGIGYLAMWFFLRRKAG"
     gene            4949..5500
                     /locus_tag="AC16PP_06605"
     CDS             4949..5500
                     /locus_tag="AC16PP_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6481333.1"
                     /translation="MEVSDETLVQQVVRLKDLQAFERLVQRHEKRILHLQRRFIRDHA
                     LAEDLCQETFLRAWDRLETFSGRGSFAAWLTKLAYNIFLQHRRKHAARTSRETPYEEE
                     AEAGQTALEGPAPNADELPDLPRLLAILPEADRLVLLLSYGHGLSIAEIADVLETPDG
                     TIKSQIHRAKDKIRRHFSIGAAA"
     gene            5497..5844
                     /locus_tag="AC16PP_06610"
     CDS             5497..5844
                     /locus_tag="AC16PP_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481334.1"
                     /translation="MSTTNPGNDPHKDPLDALLSEAFQQDIEQVGDADMAGRVMRTLK
                     RRTRLRAAILGVSMAAGLSLATALALPGLTDLLRLLAEQAPVEPSSWGVLPIAALLAI
                     LTPWLFSLVDDRV"
     gene            6009..6563
                     /locus_tag="AC16PP_06615"
     CDS             6009..6563
                     /locus_tag="AC16PP_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6481335.1"
                     /translation="MTQPARETLAAMLQVHLGRLRRLALVLTENPDDAEDLVQDVVVK
                     LCGRPHEVAGVRDLSTWLNRVVYNQFIDDRRRRKRQPLQLVGSADELDAAMPGESAEP
                     MLEALCKDRDQRLGDAVNALNAEQRMVLLLHDSQGYRLTEIHQLTDIPLGTLKSRLHR
                     ARSRMRVLLDSERVPDDRSEPDQG"
     gene            6680..7633
                     /gene="cysK"
                     /locus_tag="AC16PP_06620"
     CDS             6680..7633
                     /gene="cysK"
                     /locus_tag="AC16PP_06620"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015371271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR6481336.1"
                     /translation="MIYDNILQTIGNTPIVRLNRTGPDHVEMYVKVEAFNPLASVKDR
                     LAYAIIKDATDSGALQPGQTVVEATSGNTGIALAMVCAATGHPFVATMVETFSIERRK
                     IMRALGAKVILTPAAERGSGMVRRAEELAAEHGWFLARQFENPANPAYHRNTTGPEIL
                     KDFAGRRLDYWVTGWGTGGTLTGAGEVIKVARPDVKIIAAEPEQASMLAGKEWSPHMI
                     QGWTPDFIPAVLNPEVCDAIVPVNQDVAKDTALALARNEGIFVGVSAGGTLAAALDVA
                     EDAAPGSVFVVMLPDTGERYLSTFMFEGINEGSDDDWLESL"
     gene            7703..8911
                     /locus_tag="AC16PP_06625"
     CDS             7703..8911
                     /locus_tag="AC16PP_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6481337.1"
                     /translation="MAELSLESIFTPEIIADPYPLYRQLRETSPVLELPDANMVILTR
                     YADVQTVLRDRRLGHADESMLTRGDLEQARNNPAIANLRRTMLLKNPPDHTRLRGLVV
                     KAFDARRVEAMRTRIRAIANELVDAFVDAGSGDLVRLFTHPLPVIVICDLLGVPKADQ
                     AEFVQGTRISGRLIDPSPMTEEELAQANASTTESQRYFEQLCDERRQAPRDDLITALV
                     QSETEHGKLSKEELTSNISLLFAAGHETTVNLMGNGLIALYRNRDQLDRLRADPSLMP
                     NAVEEFLRYDSSVQLTGRDALEDAEIAGIALPRGRAVLALLAAANRDPEAFDRPEELD
                     IARKRIKPVSFGGGIHLCLGAQLARIEAHEALNVLLERLPNLELDDVATPDWKQTITL
                     RGVRTLPATW"
     gene            complement(8937..9365)
                     /locus_tag="AC16PP_06630"
     CDS             complement(8937..9365)
                     /locus_tag="AC16PP_06630"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6481338.1"
                     /translation="MIELDEEIKGRLDRAIEEHKTVTVAYVDVDGKPHISFYGSTHVH
                     SSNQLAIWVRNPQNDLIRTIPDRPNVAIIYGDIGGRVYYTFEGTASVTDDAEEQERVY
                     QEMHPVERQFDPDRGGVAVVVDLDRVTLLSAAAGKQVMER"
     gene            9563..9919
                     /locus_tag="AC16PP_06635"
     CDS             9563..9919
                     /locus_tag="AC16PP_06635"
                     /inference="COORDINATES: protein motif:HMM:NF023178.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HIR6481339.1"
                     /translation="MPEILFLLVAGHAICDFVLQSEAMATGKNHRLKEKMRERHGPSF
                     PSWPYWLTSHTITHGAAVFLITGNLLLGMIETVLHTVIDYTKCAGRISLLTDQILHGA
                     CKIGYVYYLWHAGALG"
     gene            complement(9940..10068)
                     /locus_tag="AC16PP_06640"
     CDS             complement(9940..10068)
                     /locus_tag="AC16PP_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481340.1"
                     /translation="MSGQDQGGFRANETIARLVAFVGPYILWGTLGLVVWGMLLTA"
     gene            complement(10594..>11155)
                     /locus_tag="AC16PP_06645"
     CDS             complement(10594..>11155)
                     /locus_tag="AC16PP_06645"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0050427 - 3'-phosphoadenosine
                     5'-phosphosulfate metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ family
                     protein"
                     /protein_id="HIR6481341.1"
                     /translation="LDGTKEFVSRNGEFTVNIALIEGHRAVFGVVGVPTQGYVYTGDL
                     ATATALRHGPEGAVPLRGRKRNDSDPLVVVASRSHGGQRLEDYLDALARSIGEIERKP
                     VGSSLKLCILAEGHADLYPRLGPTSEWDIAAAHAVLEAAGGAVWAVGGGALSYNAKES
                     FLNPEFFAAADGSYPWRKVLPAVPAA"
BASE COUNT         2015 a         3769 c         3524 g         1847 t
ORIGIN      
        1 atgacccact ccggaatgac gtcgcctgcc tcaacttgca tcgccgctcc tcccatacgt
       61 ccaggtgatg gtgctccggg cgaggacggt gccgcccgga tcggccacgt cgaactgaaa
      121 ctggatccgg tcgtagacgc gatccccact gcggcgacga tcagcctcgg tcacgcgtgc
      181 ggagatcgag tactccacgt cctcctccag cgtgccgaag aactcccagt catagctctc
      241 gatgccgatg gagtaatcgg attccgcctg acccagggcg aacatctcgc cgatgctcgt
      301 gccggctccg agaatcggca tgtggaacat gaccaccggg tgcaccatgc cctcgggcag
      361 ctgctctgcg cccgtgcact cggtcagcag gaagttctcc cagtgagcga ccgtgtattt
      421 gccgcccgga aactcgtggc ccactagagc ctcgatttcg gacgcgtccg gcatcgtcat
      481 gccacattcc ccttcttgat ccagcgacca acattcaaac caatgaccgg gacctggtca
      541 acggacggtt cagcctgacg cgtcgggccg gtaacatctg cagaccgcaa aggagaccca
      601 gatgtcaaag atcgacccgt acgccagctg gcttcctctg gaagcgcgtg cagcggccga
      661 aaccaatccc cggcacaagc agctgctgct ggaggtacgc gaccacatgg agcacgagat
      721 caaaggcgcg ctggagccac taatggcaac gctgaccgcg gagcccgtct accatttctg
      781 gggcaacgag ccgtcggtgc tcaacggccg cgacgcggtc agggatttct acaagggcat
      841 gatcgccatg gggggtcagc agttccaggt agtcgtcgac cgcatcgtgg tggacgacgg
      901 cgccgtggtt accgaagggc aggtaaagca ggtctacaaa ggcgcagccc tgaaggccat
      961 gggaatggac gccgtgggcg gtgaacgggt ggcggacgac gatctgttca tgaccaccgc
     1021 ccagctggtc accgtctggc ccgccgacgc cgtcggcaag ctcatcggcg aggacatcta
     1081 cttcgggcac gatccgttcc gcaacgtcga gcggatcggc cgggaagatc tgcccgacta
     1141 ctaccagatc tgatgatccc caagaccaag accgcatact acgggatcgg ctggctgcta
     1201 atgctgctgg cccttacggg ccagacggcc ctggcggcca gcgccgcgca ccgcgcccag
     1261 cacggcatgg tggtcaccca gagcgccatc gcctcggacg tcggcgcccg ggtgctgcgg
     1321 gcgggcggca acgccatcga tgctgccgtg gcgaccgcat ttgcgctggc agtgacctat
     1381 ccggaagccg gcaacatcgg cggcggcggt ttcctggtgt accaacccca agagggcggc
     1441 gcggacgcct acgactttcg ggagaccgcg cccaccggat cacacccgca gatgtggctg
     1501 gacgaagacg gcgagtactc gccggcgcgg cagtacgtcg gccccgtggc cgccggcgtg
     1561 ccgggaacgg tcgccggcct gcacaaggca tggcgcgatc acgggtccct gccctgggca
     1621 gagctggtgg cgcccgccat cgggctcgcc cgcgacggtt tcgccatccc cgaggacctg
     1681 gcccgcagca tagcggccac cctgccgcag atggaggcct acccagcctc cctggctcag
     1741 ttcagccgag acggcgcgcc catggcgccc ggtgagctcc tggtgcagcg cgatctggcc
     1801 cgaaccctgg aactcatcgc cgccgatggt cccgacgcct tctaccgggg cccgatcgcc
     1861 gagcagatcg tcgcgttgat ggcctccacc gggggcctga tcaccgccgg ggatctggcg
     1921 tcctaccgag ccgtcaagcg caagccgatc cgcggcacgt accggggcta cgagatcgtc
     1981 tccatgcctc cacccagctc gggcggcgtg gcgctggtgc agacgctcaa cctgctggag
     2041 ggctacgaca tcgccgggga gggcttcggt tccgcccgca ctctgcacct gatcaccgag
     2101 gcgttgcgcc gcacgttcgc cgatcgcgcg cggtatctgg ccgacccgga cttcgtcaag
     2161 gacatgcccg tgcggcgcct gacatcgaag tcctacgccg ccgagctgcg cgaaacgatc
     2221 tcgctgaacc gcgcaggcac gtcctcaccg gagaccttcg agtggccgac ggccagcaac
     2281 gatacggccc atttctcagt cgtggacgcc cagcgcaacg ccgtgtcgct gacctacacg
     2341 ctcaacggcc ggtacggcaa ccagatggtg gtgccaggcg ccggctttct gctgaacaac
     2401 gagatgggcg atttcaatcc ggtgcccggg ctgaccgacg agcgcgggcg catcggcacc
     2461 acacccaacc tcgcggcacc cggcaagcgc atgctctcga gcatgtcgcc gacgatcgtg
     2521 ttgaaggacg gcgagctctt catggtgaca ggagcgcggg gcggacgctc catcatcagc
     2581 accgtgctgc agaccatcat gaacgtggtc gaccacggca tgaacgccca ggcggcggtg
     2641 gacgcccgtc gaattcatca tcagtggctg ccggaccgac tgcgctacga gccgcagagc
     2701 ttttcgcgcg acacgctgaa cgcgctgcgc tggctgggcc acgagataga gcagggcggg
     2761 gcatcctgga gcaacgccga ggtcatagtg gtagcgaacg acggctggct ggagggcggc
     2821 gacgaccgcc gacgctcccg cgggtccgtg gccgggtact gagcgcacgc ggcgaccgtt
     2881 tcaactcaat gaggagcaac gacatgaaga tcgggctgtc caactttccc accgaatatt
     2941 ccatctccgc cgtggccctt gcccgagcgg ccgaggaacg cggcttcgag tccttgtggg
     3001 tggtggagca cacccatata cccgcctccc ggcggacgcc ctacccaata ggcggcgacc
     3061 tgccgagcat ctactgggaa agctacgagc cgttcacgtt cctcgctcag gcagccgccg
     3121 tgaccaccga cctgcgggtc ggcaccggta tctgcctggt cccggagcat caccccatcg
     3181 ccctggcaaa acgggtggcg tccctggacg ccctgtcggg cggacgcttc ctgttcggcg
     3241 tgggcgcggg atggaacgcc gaggagctgg agaaccacgg cgtggcgttc ggcgaccgct
     3301 ggaaggtgct gcgagagcac gtcctggcca tgaaggcatg ctggaccgag aaagatgccg
     3361 agtaccacgg caggttcgtc gacttcgatc ccgtctgggt ggaacccaag ccagtgcaga
     3421 agccccatcc gccgatatac atcggggcgt cctccacctg ggccatggag cggatcgtgg
     3481 aatacggcga cggctggctg ccggtcccgg tacccgagtt cgacgagcgc cttgctctgc
     3541 tgaaccggct ctgcgaggaa cgcggccgtg accgcagcga gatcgacgtc tgcctgatga
     3601 ccgtggtgga cagcgccgac accctggccg gcatccggga caaaggcgtc aaccgggtaa
     3661 tcctgtctct gcccacgctg ggcgaggacg aggcgctgcg cgtcctggac ggctatgcca
     3721 acgtcgttga gtgggcgaag gagatcggcg acggctgagc cggccggttg ctcagggctt
     3781 tctggcaacc agccgggcga gggccaccct gcgcccatcg gggtcgtcgg taaccgtgtc
     3841 cgttgcctcg agcacctcga gatcgggcac cgcggcgcgc agcgcgcccg gcatcacccg
     3901 aaagcgagca tcccgcggac caatcaccgg ctcttcggtg gtcagatgct cctcgacgat
     3961 caggacccct ccagggcgca gcaatgccgg cagcgctcgt agcagcgact cgtcgacgta
     4021 gcgaatcatg acgatgaggt cgtagggccc ctccacgggc aggccggtgt ccaggtcgcg
     4081 ctgcatccag ttcacctcga gcccggcctc ggcggccgcc gaggctgccc tggccagagc
     4141 ctctgcggag acatcaacgg catcgacccg aaaaccagcg cgagccatgt gcagggcgtt
     4201 gcggcccgcc ccgcaggcga tgtccagagc atcaccggct gaaagccggg gcagccaatc
     4261 cgcgaggatg gcgctggggt gcggacgatc ggcgtaagcg ccggccgcat agcgctgatc
     4321 ccacttcttc cggtcctgat ccgccacggg ctctccaggt gatcgggcgt tcacgtgatt
     4381 ttcacagcgt ccggggcaca tttcaccctg gacgccccgg gaaccggccc tcccctctcc
     4441 ccacctcggt cccggggcat ccttcatctc cccccaccgt ctccgacttt tcgctcccgg
     4501 catgcaaccg gaagcggaaa acgtgcatca gtccagttag agccacagaa aattacggag
     4561 acagatcgtg gaagacattc tcgtgcccat ctcgctgttc ctcgtcatag gagccgtcat
     4621 ctcactgtcc ttttacttcc gataccggac ccgccaggaa atccagagca cggtccgggc
     4681 ggccatcgag cgcggccagg agttgacgcc cgaggtgctc gacggcctct ccgatgccct
     4741 gaactcccgc cacggcgacc tgcgtcgcgg aatcatttcg ctggccatcg gcatcgcgct
     4801 tttccttttt gcagggcttc tcggcgagcc ggacgcggag ggtcccctga tggccatttc
     4861 tgccttcccg ctgctgctgg ggatcggcta tctcgcgatg tggttcttcc tgcgccgcaa
     4921 agccggatga cagtggtcta atggcgtgat ggaggtcagc gacgaaaccc tggtacagca
     4981 ggtcgttcgg ctgaaggatc tgcaggcgtt cgagcgcctg gtccagcggc acgagaagcg
     5041 catcctgcac cttcagcgcc gcttcatcag ggaccacgcc ctggcggagg acctctgcca
     5101 ggaaacgttt ctccgggcgt gggaccggct ggaaaccttc agcgggcggg gcagtttcgc
     5161 tgcctggctc accaagctgg cctacaacat ctttctgcag caccgcagaa agcatgccgc
     5221 cagaacgagc cgtgaaaccc catacgagga agaggctgag gcagggcaga cggcgctcga
     5281 gggcccggcg ccaaatgccg acgagttgcc ggacctgcct cgtctgctgg cgatacttcc
     5341 cgaggccgac cggctcgtgc tgctgctgag ttacggccac gggctgagca tcgcagaaat
     5401 cgccgacgtt ctcgaaacgc cggacggtac gataaaatcc cagatccacc gggcaaaaga
     5461 caagattcgc aggcacttca gcataggggc cgcagcatga gcactaccaa tcctggcaac
     5521 gatcctcaca aggatcctct cgacgccctg ctcagcgagg cgttccaaca ggacatcgag
     5581 caggtcggcg atgcagacat ggctggccgg gttatgcgaa ctctcaagcg gcgcaccagg
     5641 ctgcgcgccg ccatcctggg ggtgagcatg gcagcgggtc tttcgttggc aacagccctg
     5701 gccctgccgg gccttaccga cctgctgcgc ctgctcgcag agcaggcccc cgttgagccc
     5761 tcttcctggg gagtcctgcc catcgctgcg cttctggcca tcctcacgcc ctggctcttc
     5821 tccctggtgg acgaccgcgt ctgacctgaa ggagcgcatc gcgacggcgc gctcgctcaa
     5881 ctttgcgggc tcgcgcacac ttttcccttc cgccaggcac ccattgtggg cacacgttcg
     5941 agcctttgct ggtccgaggt gcaacactga tccgcgatcg cagtttgatc aggaccacga
     6001 aacctcccat gactcaaccc gctcgggaaa cccttgcagc catgctgcag gttcatctgg
     6061 gcaggctccg acgcctggcc ctggttctca cggaaaaccc ggacgacgcc gaggacctgg
     6121 tgcaggacgt ggtagtcaag ctctgcggac ggccgcacga agtcgctggc gtccgggatc
     6181 tttccacatg gctcaaccgc gttgtctata accagttcat cgacgaccgg cgccggcgaa
     6241 agcgccagcc gctgcagctg gtcgggtcgg ccgacgagct cgatgccgcc atgcccggcg
     6301 agtccgccga gccgatgctg gaggccctct gcaaagaccg cgaccaacgc ctgggggacg
     6361 ccgtgaacgc tctgaacgcc gagcagagga tggtgctgct cctgcacgat tcgcagggct
     6421 accgtctcac cgagattcac cagcttacgg acattccgct gggcactctg aaatccaggc
     6481 tgcatcgggc ccgctcccga atgcgcgtgc tgctggacag cgagagggtc ccggacgacc
     6541 gatcagaacc tgaccagggc tgaccgaacc gccccggaag aactcgaaac gccccgaccg
     6601 gctacgccca cctggatgag gcaaacccca ttggggtatc gtgggctgca gcgaagttcg
     6661 cacacggagt ggcatcccga tgatttacga caacatcctg cagaccatcg gcaacacgcc
     6721 gatcgtcagg ctcaatcgga ccggccccga ccacgtggag atgtacgtga aggtcgaggc
     6781 gttcaacccg ctggcatccg tgaaggaccg cctcgcctac gccatcatca aggacgccac
     6841 ggactccggc gcgctgcagc cgggacagac cgtggtcgag gccacgtcgg gcaacacggg
     6901 catcgcgctc gccatggtct gcgcggccac gggacatccg ttcgtcgcca ccatggtgga
     6961 gacgttctcc atcgagcggc gcaagatcat gcgcgccctg ggcgcgaagg tcattctcac
     7021 acccgccgca gagcgcggca gcggtatggt ccgtcgagcg gaggagctgg ctgccgagca
     7081 cggctggttc ctggcgcgac agttcgagaa cccggccaat ccggcctacc acagaaacac
     7141 caccggcccc gagatcctga aggacttcgc cgggcgccgg ctggactact gggtgaccgg
     7201 ctggggcacc ggcggcacgc tgacgggcgc cggggaagtg atcaaggtcg cccgccccga
     7261 tgtgaagatc atcgccgccg agccagagca ggcatccatg ctggcaggca aggagtggag
     7321 cccgcacatg attcagggat ggacgcccga cttcatcccc gcggtgctca atcccgaggt
     7381 gtgcgatgcg atcgttcccg tcaatcagga cgtggcgaaa gacaccgcgc tggcgctggc
     7441 gcgcaacgag ggcatcttcg tcggcgtttc cgccgggggg acgctcgccg cggccctgga
     7501 cgtcgccgag gacgcggcgc ccggcagcgt gttcgtcgtg atgctgccgg acaccggaga
     7561 aaggtatctt tccaccttca tgttcgaggg catcaacgaa ggttcggacg acgactggct
     7621 cgagtccctg tgatcggccg ccgagtcatg ccgccgagct gccctgacaa ccggcaggtc
     7681 ggcgggcgaa cggagagtaa caatggctga attgagcctc gaatcgatct tcacgcccga
     7741 gatcatcgcg gacccctacc cgctataccg gcagcttcgc gagacgagcc cggttctcga
     7801 gcttcccgac gccaacatgg tgattctcac ccgctacgcc gacgtgcaga cggtcctgcg
     7861 ggatcgccgc ctgggccacg ccgacgagtc catgctgacc cggggcgatc tcgagcaggc
     7921 gcgaaacaac ccggccatcg ccaatttacg gcgcaccatg ctgctcaaga acccgcccga
     7981 ccacacccgc ctgcggggtc tggttgtcaa ggctttcgac gcgaggcggg tggaggccat
     8041 gcgcacgcgc attcgagcca tagccaacga gctcgtggac gcgttcgtcg atgccggcag
     8101 cggtgacctg gtccgcctgt tcacccatcc tcttccggtc atcgtcatat gcgacctgct
     8161 cggcgtgccc aaggcagacc aggccgagtt cgtgcagggc acgcgcattt ccggacggct
     8221 catcgacccg agccccatga cggaggaaga gctcgctcag gcaaacgcga gcaccacgga
     8281 gagccagcgt tacttcgagc agctgtgcga cgagcgccgc caggcgccca gggacgacct
     8341 gatcaccgcc ctcgtgcaga gcgagaccga gcacggcaag ctcagcaagg aggagctcac
     8401 gtcgaacatc tctttgctgt tcgcagccgg ccacgagacc accgtcaacc tgatgggcaa
     8461 cgggctcatc gccctctacc ggaaccggga ccagctcgac cggctgcgcg cagacccctc
     8521 gctcatgccc aacgccgtcg aggagttcct gcgttacgac agctccgtgc agctgacggg
     8581 ccgggacgcg ctggaagacg ccgagattgc cggtattgcc ctgccgcgcg ggcgcgccgt
     8641 cctggcgctg ctggcagccg ccaaccgcga ccccgaggcg ttcgaccgcc cggaggagct
     8701 ggacatagcc cggaagcgca tcaagccggt gtcgttcggc ggaggcatcc acctgtgtct
     8761 gggggcgcag ctggcgcgaa tcgaggccca cgaggccctg aacgtgttgc tcgaaagact
     8821 gccgaatcta gagctggacg acgtggccac gccggactgg aagcagacga tcaccctgcg
     8881 cggcgtgcgc acgctgccgg caacctggta gtcggcggcg gagagcgtcc ccccctttag
     8941 cgctccatga cctgtttgcc ggcggccgcg ctcagcaggg tgacccggtc gagatcgacg
     9001 accacggcaa cgccaccccg gtccgggtcg aactgccgct ccacggggtg catctcctga
     9061 tacacccgct cctgctcttc agcgtcgtcc gtcaccgagg cggtaccctc gaaggtgtag
     9121 tacacgcgac cgccgatgtc accgtagatg atggcgacgt tgggccggtc gggaatcgtt
     9181 ctgatcagat cgttctgcgg gttgcgcacc cagatcgcca gctggttgct gctgtgaacg
     9241 tgagtgcttc catagaagga gatgtggggc ttgccgtcca cgtccacgta ggccaccgtg
     9301 acggtcttgt gttcctcgat ggcccggtcc agccggccct tgatttcctc gtccagctcg
     9361 atcatcgcgt ctcctcctgg ggctttggct gcccgtcttt ggtctatact cgcttgccac
     9421 gagggcgacc cctgcgtcgc cgaagcgccg tcgccgcccg ggcagaccgg gggctggagc
     9481 gcttcgcgag gggggcgaaa cttaccatac tttcgatgcg gattgccggc cgcggcccgg
     9541 tcgtccacgg attggacaag caatgcctga gatactgttt cttctcgttg caggacacgc
     9601 catctgcgat ttcgtcctgc agtccgaagc catggcgacc ggcaagaacc accgcctcaa
     9661 ggagaagatg cgggagcggc acggcccctc cttcccctcc tggccttact ggctgacctc
     9721 acacacgatc actcacgggg ccgccgtatt cctgatcacg ggaaatctgc tgctggggat
     9781 gatcgaaacc gtgctccaca cggtgatcga ctacaccaag tgcgccggtc ggatcagcct
     9841 gctgaccgac cagatcctgc acggcgcctg caagatcggc tacgtgtact acctttggca
     9901 tgcgggcgcg ctgggctgat ccggcgctgg ccgccctcat cacgcggtca gcagcatccc
     9961 ccagaccaca aggcccagcg tgccccagag aatgtacggg cctacgaaag cgacgagccg
    10021 ggcaatggtc tcgttcgccc gaaatccccc ctggtcctgt ccgctcatca acttctccca
    10081 gctacggacg cctgcggagc ccccaattgc tccgcgtctc ggggcgatgt ccggctggct
    10141 gaagagagtg gcgccggcgg aacgcccgtc ttctctaggt tttatgccct tcctaactat
    10201 ttggcaatta aatttacgtg agaggatgtg cggcagatgt aacgtggtgc tgctttgcgg
    10261 aatcggggtt cctgggaaaa atcggggaaa agtgaaaata atatggattc gacaacttca
    10321 actgattgat ttcaaaggct attgccttcg actagcccat gaggccggcc agcaggcttt
    10381 ttttccggaa tgatttcggt atattggtcc acctcactag ccggtgctgt atgtaaatac
    10441 agtgtaattc tcctcggcag ggcgaacaca cgaacataag cccggctagt gaggccaacc
    10501 tcccgacctt tcaagctctc ccgaccactc tacctgctgg actgactgac gctcttctgg
    10561 agcccgtcta cggccttggg cgctggtgcc cgctcaggca gcgggcacgg ccggcagaac
    10621 ctttcgccag gggtaggagc cgtcggcggc ggcgaaaaat tccggattga gaaaggattc
    10681 cttcgcgttg taggagagcg ctccgccacc caccgcccag acggcaccac cggcagcttc
    10741 cagcaccgcg tgggctgcgg cgatgtccca ttcagaggta ggtcccaggc gaggatagag
    10801 atccgcatgc ccctcggcga gtatgcagag cttcaacgag ctgcccacgg gcttgcgttc
    10861 gatctcgccg atgctgcggg cgagcgcgtc caggtagtcc tccagccgct ggccaccatg
    10921 gctccggctg gccaccacca ccagaggatc ggagtcgttg cgcttgcgtc cgcgcagcgg
    10981 cacggcgccc tcggggccgt ggcgcagcgc cgtcgcggtg gcaaggtccc cggtatacac
    11041 atagccctgg gtcggcaccc cgacgacgcc gaaaaccgcg cggtgaccct cgatgagcgc
    11101 gatgttgacc gtgaactcgc cgttgcggct cacgaactcc ttggtgccgt cgaga
//
LOCUS       DBNHKV010000209         7548 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_879311, whole genome shotgun sequence.
ACCESSION   DBNHKV010000209 DBNHKV010000000
VERSION     DBNHKV010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7548)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7548)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7548
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_879311"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /gene="rarD"
                     /locus_tag="AC16PP_06650"
     CDS             complement(<1..595)
                     /gene="rarD"
                     /locus_tag="AC16PP_06650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HIR6481342.1"
                     /translation="MSEQRAGMLFALAAYGFWGFAPVYFKVLDFAGPVEIIAHRVLWS
                     VPLLALLIGARRQFGALLALRADQVGWLGLSGLLVAVNWGVFVWALLNGRMLETSLGY
                     YINPLVSVLLGVLFLGEWLRRGQLVAVALAAIGVVNEVVSVEVIPWAGLTLAVSFGFY
                     GLVRKRLGVDSAVGLGVEATLLLPVALGYLAYQFEAGQ"
     gene            complement(623..979)
                     /locus_tag="AC16PP_06655"
     CDS             complement(623..979)
                     /locus_tag="AC16PP_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6481343.1"
                     /translation="MKYVRIYSGDDGQSHFEDVEVDLADHGPLGRISDLVRGKGVVFR
                     EVEGEYHSDFHNAPRRQFVVNLTGSVDIEVGDGTVRRMEPGSILLAEDLTGQGHVSRS
                     VNGEPRTCLFIPLADD"
     gene            1086..1979
                     /locus_tag="AC16PP_06660"
     CDS             1086..1979
                     /locus_tag="AC16PP_06660"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6481344.1"
                     /translation="MDQTSNHVLREIVDILNVERIETNLYRGQNHQTEHVFGGQVLAQ
                     AIAAAYRTVDVPHGLHSIHAYFLRPGDWRVPILYEVDRIRDGRSFSTRRVVAVQNGRA
                     IFSMASSWQKDEEGLEHAVQMPDVPPPGALRGDREAYLEAAREHPEISRFAFRFEAID
                     SRQVERILMTDSGTHPPYKHTWLRTRETLPDDPEIHLAMLAYMSDLDFMSTSMLPHGR
                     NTIREHVQGASLDHSIWFHRPFRADEWLLFAKESPNAGGARGFVRGRFFNRDGELVAS
                     AAQECLIRPRGALAENLASPA"
     gene            complement(1989..2468)
                     /locus_tag="AC16PP_06665"
     CDS             complement(1989..2468)
                     /locus_tag="AC16PP_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HIR6481345.1"
                     /translation="MAIQEGDKLPEATMHIIQDGRPTPVSTGDLFAGKKVVVFAVPGA
                     FTPTCSQAHLPGYVVNADAIKAKGVDSIVCLSVNDAFVMDCWGQDRNAEEILMVGDGN
                     GDFTRAIGLEMDGSGFGLGTRSQRYAMIVEDGTVTKLALEEPGQFEVSKAEAILEAL"
     gene            complement(2560..3219)
                     /locus_tag="AC16PP_06670"
     CDS             complement(2560..3219)
                     /locus_tag="AC16PP_06670"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6481346.1"
                     /translation="MECDERLLELISENLARFEASDADVDDAHHAAVAITVTDVGPGA
                     DLPGLSGTDGWQSQAGLLLTRRSAQLRKHAGQWAFPGGRLDPGETPAETALREMHEEV
                     RVDLGPDRILGRLDDFVTRSGFVMTPFVVWGGAGLQASPNPDEVQSIHRIPLTEFLRE
                     DAPVLEDADSSDRPVLKMPVGTEHIAAPTAALIYQFREVCLLGRHTRVAHYEQPAFAW
                     R"
     gene            3328..4212
                     /locus_tag="AC16PP_06675"
     CDS             3328..4212
                     /locus_tag="AC16PP_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6481347.1"
                     /translation="MSHDSERQALVAALAAVLLWSTVATGFKLGLAVMAPLQLLLAGT
                     TVSAIFFAAVTTARRSWSVTGRQLGEAAAFGIVNPALYYLVLFEAYDRLPAQLAQPLN
                     YTWAITLAVLAVPILKQRLSARTLAGILVSYLGVLVLLSQGRFDRLPAVDWTGVMLAL
                     GSTLLWAGYWLMNARSRSDSVNLMALSFLIATPLVAIACVLGPGLPTLDWNVAWFGAW
                     VGLVEMGVTFLLWQRAMGLTSHAARIGQLIFLSPFISLMLISTVLGETIHWTSVAGLV
                     VIVGGVLLTRSAAPGRAR"
     gene            4280..4876
                     /locus_tag="AC16PP_06680"
     CDS             4280..4876
                     /locus_tag="AC16PP_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6481348.1"
                     /translation="MIRTTTAVVTALLALAAAPNLTASESDTIARGQYLVTLLGCGRC
                     HTEGYLTGDKATGPYLGGSRVGIAYTAYSMDDTHPGVVFAGNLTPDDATGLGTWSEEE
                     IVQAMTVGVAKSGHERLTVMPWTNYGALKEKDVRAIARYLKSLSPVRWPIPEAIPEGD
                     PIDEPYVRFGVYKFYPHGPVEAINLPVPTNEPSPEGVQ"
     gene            complement(4947..5735)
                     /locus_tag="AC16PP_06685"
     CDS             complement(4947..5735)
                     /locus_tag="AC16PP_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6481349.1"
                     /translation="MSYETLLFEVADGVGTITINRPEAFNAMSPLCAKEFADVAVRCD
                     DDPDVRAVVLTGAGKAFCAGGDLGAFASAGSSAKSLLKVMAGDLHMGLSRLARGNAPV
                     IAAVNGTAAGAGFSLVMAADLAIAAHSAVFTMAYTNAGLSPDGSSTFYMPRKIGDRRT
                     RELMLTNRLLKAEEALAWGVVNQVVADDQVVAEAMKLAQKLAAGPTLAFGAVKTLLNG
                     TFDQTLESQMELEARAIAELSVTADGQEGINAFMAKRKPEFRGA"
     gene            complement(5758..6360)
                     /locus_tag="AC16PP_06690"
     CDS             complement(5758..6360)
                     /locus_tag="AC16PP_06690"
                     /inference="COORDINATES: protein motif:HMM:NF036525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6481350.1"
                     /translation="MANERITRATTPARRASVLDAALACFSERGLEGTTVSDIQSAAG
                     CSVGSIYHHFGSKEGIAEELFILGVQRLNQRMLKRLRRCKTGEESVRAVVGEYVRWAT
                     RESAMARYLHSRDVSFSADARERLRDIHREYVEALFAWFAPFVETGEMRRLPVQVYIP
                     LISGPIQEYVRRWLSGQHTRSPQQVEALFADAAWNAVKAR"
     gene            6446..>7548
                     /locus_tag="AC16PP_06695"
     CDS             6446..>7548
                     /locus_tag="AC16PP_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6481351.1"
                     /translation="MDIELSTEDVEFRAEVRAFLKDNAYEAGTDYNEWRNGWFEKAAE
                     KGGWDVPKWPEAFGGPGWSPTQHYIWEQETAVATLPWDLPFGIGMLAPILMSYGNEEQ
                     QKRFLPDIRARKVNWCQGYSEPGAGSDLASLKTKAVLSEDGSHYTVNGTKIWTTVAHI
                     ADWIFCLTRTDDSGKKQEGITFLLFPMKQPGVEVKPIITLGGAHSVNTVFLTDVKVPV
                     ENRIGEEGKGWTYAKGLLQHERTGLAGISRSIVALENLKAQAATVIRGNGTLMDDPGF
                     KGKVAELEVDLMAVEFTELRSLASASAGAAPGPESSILKLKGTEIQQRIQKLKVAAGG
                     IYSAAWGARPVGHGFAKGGMAGYLASRAYTIYGG"
BASE COUNT         1329 a         2610 c         2352 g         1257 t
ORIGIN      
        1 cctgccccgc ctcgaactga tacgccaggt accccagcgc cacgggcaga agcagggtgg
       61 cctccacgcc cagccccacg gccgagtcca ctcccagccg cttacggacc agcccgtaga
      121 agccgaagct caccgccagg gtcaggcccg cccaggggat gacctccacg gagacgactt
      181 cgttcaccac gccgatcgcg gcgagagcaa ccgccaccag ctgcccgcga cgcagccact
      241 cgccgagaaa cagcaccccg agcagaacgg agaccagcgg gttgatgtag tacccaaggc
      301 tggtttcgag catgcgcccg ttgagcagag cccagacgaa cacgccccag ttcacggcca
      361 cgagcaggcc gctcaaccca agccagccaa cctgatccgc acgaagcgcg agcagcgcgc
      421 cgaactggcg ccgcgcgccg atcagcagcg cgagcagagg aaccgaccag aggaccctgt
      481 gcgcgatgat ctccaccggc ccggcaaaat ccagaacctt gaagtaaacg ggggcaaagc
      541 cccagaagcc gtaagcggcc agcgcgaaca gcattcccgc gcgttgctcg ctcatgtcgg
      601 tttccggaat ggcagagacg attcagtcgt ctgcaagcgg tatgaaaaga caggtgcgag
      661 gctcgccatt gacgctacgg ctgacgtgcc cctgcccggt gagatcttcg gcgagcagga
      721 tgctgccggg ctccatgcgc cgaacggtgc catcaccgac ttctatatcc acggaccccg
      781 tcaggttgac cacgaactgc cggcgcggag cattgtgaaa atcggagtgg tactcgcctt
      841 cgacttcgcg gaacaccacc cccttgccgc gcaccagatc cgagatcctg cccaggggcc
      901 cgtgatcggc gagatccacc tctacgtcct cgaaatgcga ttggccgtcg tccccgctgt
      961 agatcctcac gtatttcacg tcgctgcctc cgtagtgtcc ccacccgacc ccgatccggg
     1021 catctcatcg ggtgccatgt ccagtagaat gccaccaacc gtgcggcgca gccatcgacc
     1081 cgaccatgga tcagaccagc aaccacgtgc ttcgagagat cgtcgacatc ctgaacgtcg
     1141 agcggatcga aacgaacctc taccgcggac agaaccatca gaccgagcac gttttcggcg
     1201 gccaggtgct ggctcaggcc atcgccgccg cgtaccgcac cgtggacgta ccccatggcc
     1261 tgcactccat ccacgcctat ttcctccgcc ccggtgactg gcgggtgccg atcctgtacg
     1321 aggtggaccg gatccgggac ggccgcagct tctccacgcg ccgggtggtg gccgtccaga
     1381 acggacgggc catcttcagc atggcgtcct cgtggcagaa agacgaggaa ggactagagc
     1441 acgccgtgca gatgcccgac gtgccgccgc ccggcgctct gcgcggagac cgtgaggcgt
     1501 acctggaagc cgcgcgcgag cacccggaaa tcagccgttt cgcctttcgt ttcgaagcca
     1561 tcgacagcag gcaggtggag cggatcctga tgaccgactc gggaacccac ccgccgtaca
     1621 agcacacctg gctgcggacc cgcgagacct tgcccgacga tcccgagatc catctcgcca
     1681 tgctcgccta catgtccgac ctggacttca tgagcacgtc catgctgccc catgggcgca
     1741 acacgatccg cgagcacgtt cagggagcga gcctggatca ctcgatctgg tttcaccggc
     1801 cgttccgggc cgacgagtgg ctgctgttcg caaaggagtc gcccaacgcg ggcggcgcga
     1861 gaggtttcgt gcgcgggcgg ttcttcaacc gggatggcga actcgtggcg agcgccgcac
     1921 aggagtgcct gatccgaccg cggggcgcgc tggccgaaaa cctcgcgagc cccgcgtgac
     1981 cggcagacct agagcgcttc tagtatcgcc tcggccttgc tcacctcgaa ctgcccgggc
     2041 tcttcgagcg cgagcttggt gacggtgccg tcctccacga tcatcgcgta gcgctgggat
     2101 cgcgttccca gaccgaaacc gctgccgtcc atctcgagac cgatggcacg ggtaaagtcc
     2161 ccgttgccat caccgaccat caggatttcc tcagcgttgc ggtcctggcc ccagcagtcc
     2221 atgacgaacg catcgttcac ggacaggcac acgatggagt cgacgccttt ggctttgatg
     2281 gcgtccgcat tgaccacgta gcccggcagg tgagcctggg agcacgtggg cgtgaacgcg
     2341 cccggtacgg caaagaccac caccttcttg ccggcgaaaa gatcgccggt gctgaccggc
     2401 gtcgggcgcc cgtcctggat gatgtgcatg gtggcttctg ggagcttgtc gccttcttga
     2461 atcgccatgg ccctttaatg tccttcttct gttgagcggg tccgaaagtt tggggaaaga
     2521 cgcttcgatg gtcaacgaac gctgatcgat ccgggcggat cagcgccagg caaaggccgg
     2581 ctgctcgtag tgggcgaccc gagtgtgccg gccgagcagg cagacctccc ggaactggta
     2641 gatcagcgcc gcggtgggcg ctgcgatgtg ctcggttccc accggcatct tcagcacggg
     2701 acggtcgctg ctgtcggcat cctccagcac cggcgcgtcc tcccgcagaa actcggtgag
     2761 cggtatgcgg tggatggact gcacctcgtc aggattcggc gatgcctgaa gacccgcccc
     2821 gccccagacg acgaagggcg tcatcacgaa cccggagcgc gtcacgaagt cgtccagacg
     2881 accgaggatc cggtcggggc caagatcgac ccgcacctcc tcgtgcatct cccgcagcgc
     2941 ggtctccgcc ggcgtctcgc cggggtcgag ccgcccgccg ggaaacgccc actggccggc
     3001 atgcttcctg agttgcgccg agcggcgggt gagcagcagg cctgcctggg attgccatcc
     3061 atcggtgcct gaaagacccg gcagatcggc accggggccg acgtcggtga cggtaatggc
     3121 caccgcggcg tggtgggcgt cgtccacgtc cgcatcgctg gcttcgaagc gggccaggtt
     3181 ctcgctgatc agttccagca ggcgctcatc gcattccatc gaagcagccg tcaatttggg
     3241 cggtgtgtgg cgagccccct tcggggcccg ttcgcgaatc atgcgccctg gtgtacgctg
     3301 acgcaatttc acgccccggc gaccccattg agccacgact cggaaaggca ggcactcgtc
     3361 gccgcgctcg cggccgtgct gctgtggtcc acggtcgcca ccggcttcaa gctggggctt
     3421 gcggtgatgg cgccgctgca gctgctgctg gccggaacca cggtctccgc catcttcttc
     3481 gccgccgtca ccaccgcgcg ccgcagctgg tcggtgaccg gccgccagct gggggaagcg
     3541 gccgcgttcg gcatcgtcaa tcccgccctt tactacctgg ttctgttcga ggcctacgac
     3601 cggctgccgg cccaactggc ccaacccctg aactacacct gggccatcac cctggcggtg
     3661 ctggccgtcc ccatcctgaa gcagcgtctc tcggcgcgca ccctggccgg tatcctcgtg
     3721 agctatctgg gggtgctcgt cctgctctcc caagggcgct tcgaccgcct gcccgccgtg
     3781 gactggaccg gcgtgatgct ggccctgggc agcacgctgc tgtgggccgg ctactggctg
     3841 atgaacgccc gttcccggag cgattccgtt aacctgatgg ctttgagctt tctgatcgcg
     3901 actcccctgg tagccatcgc ctgcgtgctg ggccccggcc tgccgaccct ggactggaac
     3961 gtcgcgtggt tcggcgcgtg ggtgggtctg gtggagatgg gggtgacttt tcttctgtgg
     4021 cagcgggcca tggggctgac ctcccacgcg gctcgcatcg gccagctcat ctttctctcg
     4081 cccttcattt ccctgatgct catctcgaca gtgctgggtg aaaccatcca ctggacgtcc
     4141 gtcgccggcc tcgtcgtcat cgtcggcggc gtgctcctga cccgatctgc ggcgcccggg
     4201 cgggctaggt gattcccgcg gtatcgcgac gccgccaagt cgggcaccat cggcccactg
     4261 ccacaagtgg atgcaacccg tgatcaggac gactaccgcg gtggtgaccg ccctcctcgc
     4321 actggccgcc gccccgaacc tcacggcctc ggagtcggac accattgcca gaggccagta
     4381 cctggtcacc ctgctgggct gcgggcggtg tcataccgag ggctacctca ccggcgataa
     4441 ggccacgggt ccctatctcg gcggttcccg cgtgggcatc gcgtacacgg cgtacagcat
     4501 ggacgacacc catccagggg tggtcttcgc cggcaacctg acccccgacg acgcaaccgg
     4561 tctgggaacc tggagcgaag aggagatcgt ccaggccatg accgtgggcg tagccaagag
     4621 cggtcacgag cgcctgaccg tgatgccgtg gacgaactac ggggcgctca aggaaaagga
     4681 cgtccgggcc atcgcccgct atctgaagag cctctccccc gtgcgctggc cgatccccga
     4741 ggccattccc gagggagatc cgatcgacga gccctacgta cgcttcggcg tctacaagtt
     4801 ctacccccac ggcccggtgg aggccatcaa cctgccggtt cccaccaacg agccgtcgcc
     4861 cgaaggggtg caatgaccgc aacaagccgc gggctttcgt gcggctccag ggccgacggg
     4921 gttgcagatt ccgggcgcgc cgcgatctag gcgccgcgga actccggctt gcgcttggcc
     4981 atgaaggcgt tgatgccctc ctggccgtcg gctgtcacgg atagctcggc gatcgctcgg
     5041 gcttccagct ccatctgcga ttcgagggtc tgatcgaacg tgccgttgag cagggttttc
     5101 accgcaccga acgcaagcgt cggccccgcc gccagtttct gcgcgagctt catggcctcg
     5161 gcaaccacct gatcgtccgc cacaacctgg ttgacgaccc cccaggccaa ggcctcctcg
     5221 gctttcagca gccggttggt gagcatgagc tctcgcgttc gccggtcgcc gattttccgc
     5281 ggcatgtaga aagtcgagct gccgtcggga gaaaggccgg cattggtgta ggccatggtg
     5341 aacaccgcgc tgtgtgcagc gatggccaga tcggcggcca tgaccaggct gaatcccgcc
     5401 cccgccgccg tgccgttcac ggccgcgatg acgggcgcgt tgccccgggc aagccgcgag
     5461 agccccatgt gcaggtcccc ggccatcacc ttgagcaggc tcttcgcgct cgacccggcg
     5521 ctggcgaagg cgcccaggtc cccaccggcg cagaacgctt tgcccgctcc cgtgagcacc
     5581 accgcgcgca cgtcgggatc gtcgtcgcag cgcacggcca cgtcagcaaa ctccttcgcg
     5641 cacagcgggc tcatggcgtt gaatgcctcg ggccggttga tggtgatcgt tcccacgccg
     5701 tcggccactt cgaacagcaa cgtctcgtaa ctcatctcta cgccttaacc cgtgcggtta
     5761 tcgagctttt accgcattcc aggccgcatc cgcaaacagc gcctccacct gctgcggcga
     5821 gcgggtgtgc tgcccggaga gccagcgtcg cacgtactcc tggatgggac cgctgatgag
     5881 ggggatgtaa acctggaccg gcaggcggcg catctcgccg gtttccacga acggcgcaaa
     5941 ccaggcgaac agggcctcca catactcccg gtggatgtcc cgcagccgct cccttgcgtc
     6001 cgccgagaag ctgacgtcgc gtgagtgcag atagcgggcc atcgcagact cccgcgtggc
     6061 ccagcggaca tactcgccca ccaccgcgcg tacgctttcc tccccggtct tgcaccgccg
     6121 caggcgcttg agcatgcgct ggttgagacg ctgcacgccg aggatgaaca gctcctccgc
     6181 gatgccttcc ttgctgccga aatgatgata gatgctgccc acactgcagc cggcggcgct
     6241 ctggatgtcg ctgaccgtgg tcccctcgag accgcgttcg gaaaagcagg cgagcgccgc
     6301 atccaggacg ctcgcgcgcc gcgccggggt agtcgctcgg gtgattctct cgttggccat
     6361 ggcgtcatgg taactagaat tcagttctag taaaagattc taatcagcgt taatatccgg
     6421 cccttctcga atggaggatt cagccatgga catcgagctc tcgacggagg acgtcgagtt
     6481 ccgcgcggag gtccgcgcgt tcctgaaaga caacgcctac gaagccggca cggattacaa
     6541 cgagtggcgc aacggctggt tcgagaaagc cgccgagaaa ggcggctggg acgtgcccaa
     6601 gtggccggag gccttcggcg gaccgggatg gtcacccacc cagcactaca tctgggaaca
     6661 ggaaaccgcg gtggccaccc tgccctggga cctgcccttc ggcatcggca tgctggctcc
     6721 cattctcatg tcctacggca acgaggagca gcagaagcgg tttctgcccg acatccgggc
     6781 ccgtaaggtc aactggtgcc agggctactc ggagcccggg gccggctccg acctagcctc
     6841 gctcaagacc aaggcggttc tgtcagagga cggcagccac tacaccgtca acggcaccaa
     6901 gatctggacc accgtagccc acatcgccga ctggatcttc tgcctgaccc gcaccgacga
     6961 ttccggcaag aagcaggagg gaatcacctt cctgcttttc cccatgaagc agcccggcgt
     7021 agaggtcaaa cccatcatca ccctcggagg tgcccacagc gtcaacacgg tgttcctcac
     7081 cgacgtgaag gttcccgtgg aaaaccgcat cggcgaggag ggcaagggct ggacctacgc
     7141 aaaaggactt ctccagcatg aacgcaccgg ccttgccggg atttcccgct ccatcgtggc
     7201 tttggagaac ctgaaagccc aggccgcaac ggtgatccgc ggcaacggaa ccctaatgga
     7261 cgaccccggc tttaagggca aggtcgccga gctggaggtc gacctcatgg cggtggagtt
     7321 caccgagctg cgaagcctcg cttcggcctc ggcgggcgca gcccccgggc ccgaatcctc
     7381 catactcaag ctcaagggca ccgagataca gcagcgaatc cagaagctca aggtggcagc
     7441 cgggggcatc tactccgccg cctggggggc acgacccgtc ggccacggtt tcgccaaggg
     7501 cggcatggcc ggctacctgg cgagccgcgc ctacaccatt tacggcgg
//
LOCUS       DBNHKV010000210         2285 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1799530, whole genome shotgun sequence.
ACCESSION   DBNHKV010000210 DBNHKV010000000
VERSION     DBNHKV010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1799530"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="AC16PP_06700"
     CDS             <1..355
                     /locus_tag="AC16PP_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007158956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HIR6481352.1"
                     /translation="VNPHRCSNYVADRGSFSVQEGDARIVLQPEKRRYLAGGNVMTEA
                     AIDAGFTRDLYISLGEPLQGGDWAVRIQVKPFVRWIWLGGLLMAFGGCLAVFDSRYRR
                     LKEKLLSAQVAEAAR"
     gene            352..891
                     /locus_tag="AC16PP_06705"
     CDS             352..891
                     /locus_tag="AC16PP_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HIR6481353.1"
                     /translation="MRRLRLFVPLLVFVIIVGFGYAGFKLNDPRELPSALLDRPFPEF
                     SASLLGGAGTVSRDALLGSPVLVNVWATWCPTCRAEHDELMRIRAETGLRLVGVNYKD
                     DPVKALRWLADFGDPYEFTLQDADGSLGVELGVYGAPESFLLDARGVIVYKRVGEVNE
                     RVWKEEILPALERLHDENG"
     gene            884..1342
                     /locus_tag="AC16PP_06710"
     CDS             884..1342
                     /locus_tag="AC16PP_06710"
                     /inference="COORDINATES: protein motif:HMM:NF015853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HIR6481354.1"
                     /translation="MADRRRAAIFGLLVAAGAALGAGPVDVYDFPDPATEARYRALIA
                     EFRCPKCLNTNLAGSDAPIAKDLRRTVHRLVVVEGMSDAEVREFLQSRYGDFVLYDPP
                     FRFDTSILWLAPGLLLLVGLGVLYRVLRQPPPPKLSETDARRLREILEES"
     gene            1339..>2285
                     /gene="ccmI"
                     /locus_tag="AC16PP_06715"
     CDS             1339..>2285
                     /gene="ccmI"
                     /locus_tag="AC16PP_06715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HIR6481355.1"
                     /translation="MSFWLMAGLMMLAGVLIVVVPLLRGGLQGGRTDRSVAVRSLYQE
                     RLAELDVDVAAGQLSPASRDAVAEELGSALLGDFEQTASGQGVDSRPVSPAVVLTLAV
                     LLPLVVLIVYLEVGDPGAQQIAGAEALLAMDPRTQRAEVAGWRDRLDSRVERRSDDAS
                     SWYLLGLANLQLGSFGRAAQAFAMSHALSGESPVVDAYWLQARYLSSGGEIDRETRVI
                     GDRLLKAQPNQPLVLEIYAIDAYRRGDYREAVALFSRALSGNLETEQRAVLAAGLQQA
                     RARLGDLVPSLDVSVTADGASPAGWTLFVIARPPGGGMP"
BASE COUNT          316 a          725 c          805 g          439 t
ORIGIN      
        1 cgtcaatccc catcgttgtt ccaactacgt ggccgaccgg ggcagcttca gcgtgcagga
       61 gggcgatgcc cgcatcgtgc tgcagcccga gaagcgtcgc tacctggcgg gcggcaacgt
      121 catgaccgag gccgccatcg atgctgggtt cacgcgcgac ctttacatct ccctgggcga
      181 accccttcaa ggaggtgatt gggccgttcg cattcaggtg aagccgttcg tgcgctggat
      241 ctggctgggc gggcttctga tggctttcgg cggctgcctg gcggttttcg attcgcgcta
      301 ccggcggctc aaggagaagc tcctgtcggc tcaggtcgcg gaggcggcgc gatgagacgg
      361 ctgcgcctgt tcgtgccgct gctggtgttc gtcatcatcg tcggtttcgg ctatgcgggc
      421 ttcaagctca atgatcctcg cgagctcccc tcggcgctgc tcgatcgtcc gtttccagag
      481 ttctcggctt cgcttctcgg gggagcgggc accgtaagcc gggatgctct gctgggctcc
      541 ccggtgctgg tcaacgtatg ggcaacctgg tgtcccacct gccgggccga gcacgacgag
      601 ctgatgcgca ttcgcgccga gacggggctc cgcctcgtgg gggtgaacta caaggacgac
      661 ccggtcaagg cgctgcgctg gctcgccgat ttcggggacc catacgaatt tacgcttcag
      721 gacgcggacg gatccctggg ggtggagctc ggcgtctacg gggctccgga aagctttctc
      781 ctcgatgcca ggggggtgat cgtctacaag cgggtcgggg aggtgaacga gcgcgtctgg
      841 aaagaagaaa tcctgcccgc gctggagcgc ctgcacgatg agaatggctg atcgtcgtcg
      901 cgcggccatt ttcggcctgc tcgttgctgc gggtgcggcc ctgggggccg gcccggtgga
      961 cgtctacgac tttcccgacc cggctacgga agcgcgctat cgtgcgctga ttgcggagtt
     1021 tcgctgtccg aagtgtctga acaccaacct cgccggctcc gatgcgccca ttgccaagga
     1081 cctgcggcgc acggtgcatc ggctcgtcgt cgtcgagggc atgagcgacg ccgaagttcg
     1141 cgagtttctc cagtcgcgtt acggggattt cgtgctttac gacccgccct ttcggttcga
     1201 cacgtcgatc ctctggctgg ctcccggcct cctgttgctc gtaggccttg gggttctgta
     1261 tcgcgtcctc cggcagccgc cgcccccgaa gctcagcgag acagacgccc ggcggctgcg
     1321 tgagatcctg gaagaatcat gagcttctgg ctgatggcgg ggctgatgat gctcgcaggt
     1381 gtgctcatcg tggttgtccc gctgctgcgc ggagggcttc agggcggccg gacggacagg
     1441 agcgttgcgg tccgatccct ctaccaggag cggctcgccg agctggacgt cgacgtggcg
     1501 gccggtcagc tctcgccagc atcccgcgac gcggtggccg aggagctggg ctctgctctg
     1561 ctcggcgatt tcgagcagac ggcgtccggg cagggcgtcg attccagacc ggtgtcgccc
     1621 gccgtcgtgc tgacgctcgc ggtgctgttg cccctggtgg tgctgatcgt ctatctcgag
     1681 gtcggagatc cgggcgcgca gcagatagcg ggcgcggaag cgcttctcgc catggacccg
     1741 cgcacccagc gcgccgaggt cgccggttgg cgagaccggc tcgatagccg cgttgagcgg
     1801 cgctcggacg atgccagcag ctggtatctc ctgggccttg cgaatcttca gctgggcagc
     1861 ttcgggcggg cggcgcaggc gtttgccatg tcccacgcgc tgtcgggcga aagcccggtg
     1921 gtcgacgcct actggctcca ggctcggtac ctgagctccg ggggcgaaat tgaccgggaa
     1981 accagggtca tcggcgatcg tctgctgaaa gcgcagccca accagccgct ggtgctcgag
     2041 atttacgcca tcgacgccta tcggcgcggc gactatcggg aggcagtggc tctgttctcg
     2101 cgggctctct cgggaaacct ggagaccgaa cagcgcgccg tgctcgcagc cgggctgcag
     2161 caggcgcgag cccgcctcgg agacctggtt ccgagcctgg acgtgtccgt gaccgcggac
     2221 ggcgcctcgc ccgcgggctg gacgctgttc gtcattgccc gcccgccggg aggcgggatg
     2281 ccgta
//
LOCUS       DBNHKV010000211         6208 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1447213, whole genome shotgun sequence.
ACCESSION   DBNHKV010000211 DBNHKV010000000
VERSION     DBNHKV010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6208)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1447213"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1162)
                     /locus_tag="AC16PP_06720"
     CDS             complement(56..1162)
                     /locus_tag="AC16PP_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6481356.1"
                     /translation="MSRIELDGVSHRYPEAPSETFAELELVIGSGEAHALLGSSGAGK
                     TTLLNLLSGIEQPTTGRITFDGRDVSRTPPRARNVAQVFQFPVLYESLSVAENLEFPL
                     KTRGVRAAERRRRAKAMAERLGIAELMGRRPGKLSLFERQLVAIGRALVRPELSLVLL
                     DEPLTAVEPSIKWQLRRELKAVQQEIGATMIYVTHDQTEALTFADRISLLHRGRILQT
                     GSARTLFSAPAHEQVGHFIGSPGMNLVPGEIAEGALRLGGRVVAPAAGAEPGPCTIGF
                     RPEWASVAPSGPDGSGLLGTITAARVLGVRRGVPVGILSVDLGVGVVQVRQALSASPG
                     ERVGVNVDAGQVVAFREGWRLPGIGAETGRGAGS"
     gene            complement(1149..2129)
                     /locus_tag="AC16PP_06725"
     CDS             complement(1149..2129)
                     /locus_tag="AC16PP_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6481357.1"
                     /translation="MLKLQGIRLAEGAAPLDHEFRSGQITVVLGRNRSGKTRLCRLLA
                     GLEPTGEGRILLDGVDVSNIPAGQRPVSFVYQSFVNYPTLTVAENIASPMRARGLPSR
                     ERRRRVEDLAARLHISELLDRRPAQLSGGQQQRLAIARALAKQARVLVMDEPLVNLDF
                     KLREALELELRGLLRDAGIAVVYTSSDPRDAFNLGDEVLLLADHAPVQAGRPLDVYER
                     PVSVAAADLMSDPGVNLLPGPDRPAAIRPEHLHVSRHARDDETFAAELLTLETNGSET
                     YLHCRVADAHWVVKLEGLIDLKLRPGDTLPLFAEQRSLLRFSEAMTDVQD"
     gene            2236..2754
                     /locus_tag="AC16PP_06730"
     CDS             2236..2754
                     /locus_tag="AC16PP_06730"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6481358.1"
                     /translation="MDELFETYDEAGRAMGLMARHRVHATGAWHRSAHVFLFDTTGAL
                     LIQRRAPGKDLYPDLWDYSVGEHLVPGETYLAAARRGLEEELGVRGVSLEPIGDVRRW
                     RSHQAELNLRDNELQQAFTGVHDGPFEPDAAEVAEVRAVGLQELAAEIERWPARFTPW
                     FVRDLAELGFVK"
     gene            2841..3881
                     /locus_tag="AC16PP_06735"
     CDS             2841..3881
                     /locus_tag="AC16PP_06735"
                     /inference="COORDINATES: protein motif:HMM:NF015715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HIR6481359.1"
                     /translation="MADVPTPASRLARYRTDYLLMTCAAIALVLLILALTGAAPMGTP
                     LGHAAHTVHEMLAAMWWGIALAILIIGVLERIPQEFIIALLGPPGSRNGVLRAAVAGV
                     LLDLCSHGILAVGAKLYQRGASAGQVMAFLIASPWNSVSLTFILVGLIGLGWTIAFIG
                     LSLVVAITTGLAFDRLARTGRIPANDAQVDLPVNFRFGPASRELVSTIEWEPRLLADI
                     VINGLRGTRIVLRWLMFGLVVAAALRAFLSPEDFAQWFGPSLLGLGLTLVGATIFEVC
                     SEGSVPIAADLMNRAGAPGNSFTFLLAGVASDYTEIMVLREATGSTKIALMLPAICVP
                     QVLLIGWVLNQL"
     gene            complement(3912..>6208)
                     /locus_tag="AC16PP_06740"
     CDS             complement(3912..>6208)
                     /locus_tag="AC16PP_06740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HIR6481360.1"
                     /translation="ATYRIHAGVPVIYLFGRLESGETFLVRDHRQRPHFYVRQEDARR
                     AEALDGIALEASAARTFSGDAVCRVTVPTPSDAPPARERLHGLGVDTFEADVRFAMRY
                     LIDRGIRGGCRIRGAASRGEGVGWVLDDPEVGAAKAGIEPVVLSFDIETDAKADRLLG
                     IAVYGAGVDEVLIVDPERRPMPERARGLPSERAVLEAFCQLVRDVDPDVLTGWNVVDF
                     DLTVLGRVAARVRHPFQLGRQAGALRIRPAQGYFGSGNATIPGRLVLDGMDLLRGAFV
                     RMEEYSLDAVAREVLGEGKTLDGDVRDRAGEIMQRYRQDLPGFARYARTDARLALDIV
                     TRLDLMNLAFARSRLTGMTPDRVAASIASFDFLYLSALGKRGLVAPTVRSDDSRVHAA
                     QSGGQVFEPVTGVHRNVMVFDFKSLYPSIIRTFNVDPLGFVPSGDAEPDVIRLVNGAG
                     FRRDPAILPRMLDDLFPRREAAKTAGDSVASQAIKILMNSFYGVLGTPACRFHNVEIA
                     NAITGQGRHLLQWSKRWFETRGFDVLYGDTDSLFVRSRQGDEDAALAEARDLAARLNA
                     DIAAEVLERWGVVSRLELEFEKLYAKLFLPSVRHGAAGARKRYAGLPVGAEEVEFVGM
                     EVVRRDWTELAKRVQRELYARLFSGAAVDDYLAGVVAQLRRGELDELLVYRKGLRKRL
                     DSYTASNPPHVAAARKSGEPPGRTVAYLVTSAGPEPLDALQHEPDREHYVDKQIKPVA
                     EPVLGALGLEFARVIGDDRQMGLF"
BASE COUNT         1039 a         2203 c         2009 g          957 t
ORIGIN      
        1 accagcacca ccgggagaac gccgagccag gcccggttgt cgggccgacg tccgctcacg
       61 aacctgcccc ccgtccggtc tcggcaccga ttcccgggag gcgccagcct tcccggaagg
      121 cgacgacctg cccggcgtcc acgttcacac ccacacgctc ccccggggaa gcggacaaag
      181 cctggcgaac ctgcacaacg cctacgccga ggtcgacgct gaggatcccg acgggcaccc
      241 cacgtcgcac gccgagcacc cgggccgcgg tgatcgtccc gagcaggccc gatccgtcag
      301 gcccggacgg cgccaccgat gcccactccg ggcggaagcc gatggtgcac ggtccaggct
      361 ccgcacccgc cgccggggcc accacgcgcc ctccgagccg caacgcgccc tcggcgatct
      421 cgccgggaac gaggttcatc cccggcgagc cgatgaagtg tcccacctgc tcgtgagcgg
      481 gcgcgctgaa cagggtgcgg gccgagccgg tctgcaggat ccgcccgcgg tgcagaaggc
      541 tgatgcggtc agcaaaggta agcgcctcgg tctggtcgtg agtcacgtag atcatggtgg
      601 cgccgatctc ctgctgcacg gccttcagct cccgcctgag ctgccacttg atcgaaggct
      661 ccaccgccgt cagcggctcg tcgagcagca cgagggacaa ctcgggcctg accagcgcgc
      721 gaccgatggc cacgagttgc ctttcgaaca gggacagctt gccgggccgg cggcccatca
      781 gctccgcgat gcccaggcgc tccgccatgg ctttcgcgcg gcgacgccgc tcggcggcgc
      841 gcacgccacg ggttttcagg ggaaattcca ggttctccgc cacgctgaga gattcgtaaa
      901 gcaccggaaa ctggaacacc tgggccacgt tgcgagcgcg cggcggggtg cgcgagacgt
      961 cccggccatc gaaggtgata cgcccggtcg tcggctgctc gatgccggag aggaggttga
     1021 gcagcgtcgt cttgcccgcg cccgagctgc ccagcagcgc gtgggcttcg ccggagccga
     1081 tgaccagctc gagctcggca aacgtttcgc tgggcgcctc cggatatcgg tggctgacgc
     1141 cgtccagctc aatcctggac atcggtcatc gcctccgaga atcgcagcag ggagcgctgc
     1201 tcggcgaaaa gcggcagcgt atcccccggc cggagcttca gatcgatcag gccctccagc
     1261 ttcaccaccc agtgcgcgtc cgccacccgg cagtggagat aggtctccga cccgttggtc
     1321 tcgagggtga ggagctcggc agcaaacgtc tcgtcatccc ttgcgtggcg gctcacgtgc
     1381 agatgctcgg gacgaatcgc cgccggccgg tcaggcccgg gcagcagatt gacaccgggg
     1441 tcgctcatca ggtccgcggc agcgacgctg accggacgct cgtagacgtc cagcggccgg
     1501 ccggcctgca cgggtgcgtg gtcggcaagc aggagcacct cgtcgcccag gttgaaagcg
     1561 tcccgggggt ccgagctcgt atagaccacg gcgataccgg catcccggag cagcccgcgc
     1621 agctccagct ccaacgcctc acgaagcttg aaatcaaggt tcaccagcgg ctcgtccatg
     1681 accaggacgc gcgcctgttt ggccagcgcc cgggcgatcg ccagacgctg ctgctggcca
     1741 ccggacagct gggccgggcg ccggtcgagc agctccgaaa tgtgcagcct tgccgccagg
     1801 tcctcgaccc gccggcgacg ctcacgcgac ggcaagcccc gggcccgcat gggcgaggcg
     1861 atgttctccg ccacggtgag ggtggggtaa ttgacgaacg actgatacac gaaagagacc
     1921 ggccgttggc ccgccgggat attcgagacg tcgaccccat ccagcaatat ccggccctca
     1981 cccgtgggct ccaggccggc cagcaatcgg cacaggcgcg tcttgcccga gcggttgcgg
     2041 ccgaggacca cggtaatctg ccccgaccgg aactcatggt ccaggggcgc ggcgccctcg
     2101 gccagacgga ttccctgcag cttcagcacc gctgtcctgg acccgccggc cgcctgcgtc
     2161 gctgggacat cattgacctc gacgtgcaaa ggccaaagta tagcgtccgc catccccagc
     2221 agaatcgaag gaccattgga cgagctgttc gaaacctatg acgaggcggg cagggccatg
     2281 gggctcatgg cacgccatcg ggtccacgct accggtgcct ggcaccgctc tgcccacgtc
     2341 tttcttttcg acaccaccgg cgcgctgctc atccaacgcc gcgcgccggg caaggacctc
     2401 tatcccgatc tgtgggacta cagcgtcggc gagcacctgg tgcccggcga gacatatctg
     2461 gccgccgccc ggcgcggcct agaggaggag ctcggcgtaa ggggcgtttc gctcgagccg
     2521 attggcgacg tgcggcggtg gcggtcacat caggcggagc tgaacttgcg ggacaacgag
     2581 ctccagcagg cgttcaccgg cgtgcacgac ggccccttcg agccggatgc cgccgaggtc
     2641 gccgaggtcc gcgccgtcgg ccttcaggag ctcgccgcgg aaatcgaacg gtggcccgct
     2701 cgcttcaccc cctggtttgt cagggacctc gcggagctgg gcttcgtgaa gtaggcgcct
     2761 cgacttgtcg agcaacctca cttgccagca ggacaggacc aggcgtagcc tcaaccctct
     2821 tcgctcgatg ggacgcccaa gtggctgacg tgccgacgcc cgccagccgg ctggccagat
     2881 accgcaccga ttacctgctg atgacctgcg cggccatcgc gctggttctt ctgatcctgg
     2941 cgctgacggg cgccgcgccc atggggaccc cgctcggcca tgcagctcac accgtgcacg
     3001 agatgctcgc ggccatgtgg tggggcatcg cgctggccat cctcatcatc ggcgtcctgg
     3061 agcggatacc ccaggagttc atcatcgcgc tgctcggacc tcccggaagc cggaacggcg
     3121 ttctgcgggc cgctgtcgcc ggcgtgctgc tcgacctgtg cagccacggg atcctcgccg
     3181 tcggggccaa gctctaccag cggggcgcaa gcgccgggca ggtcatggca tttctcatcg
     3241 ccagcccgtg gaactccgtt tctctgacct tcatcctcgt cggcctcatc gggctcggct
     3301 ggaccatcgc cttcatcggc ctgtcgctgg tggttgccat caccaccggc ctagcgttcg
     3361 accgcctggc ccgcacggga cgaatcccgg ctaacgacgc ccaggtcgat cttccggtga
     3421 acttccgttt cggtccggct tcccgtgagc tggtcagcac catcgagtgg gagcctcgcc
     3481 tgctcgcgga catcgtcatc aacggactgc gcgggacccg tatcgtgctc cgctggctga
     3541 tgttcgggct agtcgtcgct gccgcgttga gggcctttct cagccccgag gacttcgcgc
     3601 agtggttcgg accaagcctg ctaggcctgg gcctcaccct cgtcggcgcg accatcttcg
     3661 aggtctgctc cgagggctcc gtgcccatcg ccgcggatct gatgaaccga gcgggggccc
     3721 cgggaaacag ctttaccttc ctgctcgccg gcgtggccag cgactacacc gagatcatgg
     3781 tcctgcgcga agccaccgga tcgacgaaga ttgccctgat gctgcccgcc atctgcgttc
     3841 cccaggttct gctgatcggc tgggtgctga accagctctg accaggtgcc ggcgggcggg
     3901 agccctcccc ttcagaacag ccccatctgc cggtcgtcgc cgatcacccg ggcgaattcc
     3961 agacccagcg ccccgagaac gggctcggcc accggcttta tctgcttgtc gacgtaatgc
     4021 tcccgatcgg gctcatgctg cagcgcgtcg agaggctcgg ggcccgcgct ggtcacaagg
     4081 taagccacgg tgcgcccagg cggctctccc gactttcgcg ccgcagccac gtggggaggg
     4141 ttgctcgccg tgtagctgtc cagtcgcttc ctgagccctt tgcgatagac cagaagctcg
     4201 tccagctcgc cgcgacgcag ctgcgcgacg actccggcca ggtagtcatc cacggcggct
     4261 ccggagaaga gcctggcata cagctcccgc tgaacacgct tggcaagctc ggtccagtcc
     4321 cggcggacga cctccatgcc cacgaactcc acctcttcgg ccccgacagg caaccccgca
     4381 tagcgctttc gcgcgcccgc cgcaccgtga cgaacagacg gcaggaagag cttggcgtag
     4441 agcttttcaa attcgagctc gaggcggctg acgacccccc agcgctcgag cacctcggcg
     4501 gcaatgtccg cattgagccg cgcggcgaga tccctcgctt ccgcaagggc agcatcctcg
     4561 tcgccctgcc gcgaccgcac gaaaaggctg tcggtgtcgc cgtagagcac gtcgaagccc
     4621 ctcgtctcga accagcgttt cgaccactgc agcagatggc ggccctgacc ggtgatggcg
     4681 tttgcgatct cgacgttgtg aaaccgacag gccggggtgc ctagcacacc gtagaaagag
     4741 ttcatgagga tcttgatcgc ctgtgaggca acgctgtccc cagctgtctt cgcagcctcc
     4801 cggcgcggaa acaggtcatc gagcatgcgc ggcaggatgg ccggatcccg ccggaatcca
     4861 gcgccgttaa cgagccggat cacgtcaggc tccgcgtcgc ccgatggcac gaaccccaga
     4921 ggatcgacgt tgaaagttcg gatgatgctc gggtagaggc ttttgaaatc aaagaccatg
     4981 acgttgcgat gcacgccggt cacgggctcg aaaacctggc ctcccgactg cgcagcgtgg
     5041 acgcggctgt catcggaacg caccgtgggc gcaaccaggc cccgcttgcc cagagcggac
     5101 aggtaaagga agtcgaaaga ggcgatgctg gcagccaccc gatccggcgt catgcccgtc
     5161 agccggctgc gagcgaaagc caggttcatg agatcgaggc gagtgacgat gtccagcgcc
     5221 aggcgcgcat ccgttcgcgc atagcgcgca aaccccggca gatcctggcg gtagcgctgc
     5281 atgatctccc ccgcacggtc gcgcacgtcg ccatccagcg ttttgccctc gccgaggacc
     5341 tcccgcgcca cggcgtccag cgaatattct tccatccgca cgaacgcccc gcgaagcagg
     5401 tccatgccgt cgagcaccag ccggccggga atggtcgcgt tgccgctccc gaagtaaccc
     5461 tgggcggggc ggatgcggag cgcgcccgcc tgccgaccga gctgaaacgg atggcgcact
     5521 ctggcggcaa ccctcccgag caccgtcagg tcgaaatcga ccacgttcca gcccgtcagc
     5581 acgtccgggt cgacgtcgcg aacgagctga cagaaagctt ccagcaccgc ccgctcggag
     5641 ggcaagcccc gggcacgctc gggcatgggc cgtcgctcgg ggtcgacgat gagaacctcg
     5701 tccacgccgg cgccgtagac ggcgatgccg agcagccggt ccgccttggc gtcggtctcg
     5761 atatcgaagg aaagaaccac cggctcgata ccggctttgg cggcgccgac ctcaggatcg
     5821 tcgagcaccc agcctacccc ttctccgcgg cttgccgccc cgcggatgcg gcagccgccg
     5881 cggatgcccc ggtcgatcag atagcgcatg gcgaagcgga cgtccgcctc gaaggtgtcg
     5941 accccaaggc cgtgaagccg ctcgcgcgcg ggcggcgcat ctgagggggt gggcacggtc
     6001 acccgacaca ccgcgtcccc ggagaacgtt ctggcggcgg acgcctcgag ggcgatgccg
     6061 tctaaggcct cggctcgccg ggcatcctcc tgccgcacgt agaaatgagg ccgctgccga
     6121 tggtcacgca ccagaaacgt ttcgccggac tccaggcggc cgaacaggta aatcacgggc
     6181 acccccgcgt gaatgcggta ggtggcct
//
LOCUS       DBNHKV010000212        12067 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1518045, whole genome shotgun sequence.
ACCESSION   DBNHKV010000212 DBNHKV010000000
VERSION     DBNHKV010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12067)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12067)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12067
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1518045"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="AC16PP_06745"
     CDS             complement(<1..454)
                     /locus_tag="AC16PP_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481361.1"
                     /translation="MDRRSFLTIAGIGTAYLLRPSLARACPTQGGSSSANWADRLGPG
                     SAMGRLGELLAPDHNGIRLPAGFSSRIVAESGNRPVSSSSYAWHAAPDGGATFAHPEG
                     GWVYVSNCEMPLGFGGASALRFDESGTLVDAYPILGRTSGNCAGGATPW"
     gene            complement(474..2006)
                     /locus_tag="AC16PP_06750"
     CDS             complement(474..2006)
                     /locus_tag="AC16PP_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HIR6481362.1"
                     /translation="MEILLPGRGPREPISDGGDWTFELIGRYDREIGAIAEQFGLDTY
                     PNQIEIIRSDQMMDAYASIGMPIGYHHWSFGKHFVDVERSYQRGFMGLAYEIVINSNP
                     CISYLMEENSMTMQALVIAHACYGHNSFFKGNYLFQTWTDASAIVDYLLFARNYIASC
                     EERYGVQEVEMILDSCHALMNYGVDRYKRPYPISAEEEKRRQAERAEYVQRTLNDLWR
                     TLPQARQLDSSDEEVFPREPQENLLYFIEKHAPLLEPWQREIVRIVRKVAQYFYPQRQ
                     TQVMNEGWASFWHYTLLNELYDRGLVTDGFMMEFLASHTAVLFQPSFDHPAYRGINPY
                     ALGFAIYSDLRRVCEHPTDEDREWFPDIAGGNWLEVLPAAMRDFKDESFVLQFLSPRV
                     MREMRLFGVVDDDAEDEVLISAIHDRGGYREVRSLLAGQYNIGDREPNIQVVRVDVRG
                     DRSLTLRHAQHARQPMGQSTDEVMRHLHRLWGFPVRLESVWHDEVVATTACPAEEAQS
                     AA"
     gene            complement(2006..3274)
                     /locus_tag="AC16PP_06755"
     CDS             complement(2006..3274)
                     /locus_tag="AC16PP_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009685232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HIR6481363.1"
                     /translation="MSYIIDRRENAKNKSAVNRQRFLERYKQRIKKAVSDAVNERSIT
                     DMERGEEVTIPAQDISEPVFQHGSGGRRSIVHPGNKEFIAGDRIARPEGGAGGGSGDG
                     EASNSGEGSDDFVFQLTQEEFLEYLFDDLALPNLTKRHLKGNSSFKYEHAGFTSDGVP
                     AKLSVLRSLRQAKARRIALAGEPKRLLREAREAFDQAELDGDELQRRRMRDEIERLEK
                     RLGRIPFLDTNDLRYNLHLRKPVPTSQAVMFCLMDVSGSMDQHIKDMAKRFYLLLYMF
                     LKRHYERTEIVFIRHHTVAKEVDEQEFFYSRETGGTVVSSALKLMLEVLEDRYSPNEW
                     NIYGAQASDGDNWNDDSPLCRQLLEKKILPLVQYFSYVEITKRSHQALWREYEHLAER
                     FPESFAQRQITDVSDIFPVFHDLFQKKDAA"
     gene            complement(3350..5272)
                     /locus_tag="AC16PP_06760"
     CDS             complement(3350..5272)
                     /locus_tag="AC16PP_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HIR6481364.1"
                     /translation="MGVFEHYQERFEAAREEELSLQEYLDLCKEQPATYATAAERLLL
                     AIGEPEFVDTAKDSRLSRIFSNKIIKRYPAFDDFYGMEEAIEQIVSFFRHAAQGLEEK
                     KQILYLLGPVGGGKSSLAEKLKALMQREPFYALKGSPVNESPLGLFDPVEDGAILESD
                     YGIPRRYVRGLMSPWAAKRLKEFGGDITQFRVVKKYPSILDQVALSKTEPGDENNQDI
                     SALVGKVDIRKLEDFAQNDPDAYSFSGGLCKANQGMLEFVEMFKAPIKVLHPLLTATQ
                     EANYNGTEAIGSIPFDGIVLAHSNESEWQTFRNNRNNEAFIDRVYIVKVPYCLRVREE
                     VNIYRKLIDTSSLNAAPCAPDTLRMLAQFSVLSRLKEPENSNVFSKMRIYDGENLKDT
                     DPKAKTLQEYRDTAGVDEGMDGLSTRFAFKILSKVFNFDAIEVAANPVHLMYVLEQQI
                     EQEQYPQEVRDRYLRFIKEYLAPKYVDFIGKEIQTAYLESYSEYGQNVFDRYVTYADF
                     WIQDQEYRDPETGDILDRASLNEELEKIEKPAGISNPKDFRNEIVNFVLRARASNNGT
                     NPSWLSYEKLRTVIEKKMFSNTEDLLPVISFNAKSSSDEQRKHDDFVARMVERGYTDK
                     QVRLLAEWYLRVRKSQ"
     gene            complement(5564..6376)
                     /locus_tag="AC16PP_06765"
     CDS             complement(5564..6376)
                     /locus_tag="AC16PP_06765"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HIR6481365.1"
                     /translation="MATYAIGDVQGCYAEFCRLLKKMEFDKKRDRLWMVGDLINRGPD
                     SLRVLNLVRSLGKRAVIVLGNHELHFLAIHYGGYSANRTDTFDDLLSSPARAEIAEWL
                     CQQPLLVRSNKLGYVMTHAGIPHIWDIDVAEALAGEVEKAIRGKRRKRYFESMYGNVP
                     DVWDPKLAGMDRLRCITNYFTRMRLVDKAGRLDFSFKGGLADAPKPWLPWYELRNRKP
                     LPVKLVFGHWAALQGHTGLKDVIALDTGCVWGRKLSALCLDTGETYSVRAGG"
     gene            complement(6386..6766)
                     /gene="apaG"
                     /locus_tag="AC16PP_06770"
     CDS             complement(6386..6766)
                     /gene="apaG"
                     /locus_tag="AC16PP_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HIR6481366.1"
                     /translation="MSGPASGIEVAVDARYVVGESDPIRNRYVFAYTITISNLGPEPG
                     QLLDRHWFIADGDNEVREVQGPGVVGEQPRLEPGTAFRYTSAAVLETPVGTMHGLYGF
                     RRDDGTRFEVPIPMFSLAAPNAVH"
     gene            complement(6763..7479)
                     /gene="rsmA"
                     /locus_tag="AC16PP_06775"
     CDS             complement(6763..7479)
                     /gene="rsmA"
                     /locus_tag="AC16PP_06775"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003206170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR6481367.1"
                     /translation="MADLIRLGPHDQVLEIGPGTGALTAALLDASGAAPARYLAVEID
                     RDLAPELGRRFPTVEVVNADVLRVPLDELLGSQSWRVVGNLPYNISTPLLVRLFACTD
                     RIRDMHFMLQREVALRLAAVPGTKAWGRLTVLAQYHCQVELLFEVAPESFSPPPKVWS
                     AVARLVPHAAKLPLENPDLLDLVLRHAFSGRRKRLGNALKSLSMPWHELDVDDAARPD
                     QLSVKDFVTLANAVQLEQTP"
     gene            complement(7526..8506)
                     /gene="pdxA"
                     /locus_tag="AC16PP_06780"
     CDS             complement(7526..8506)
                     /gene="pdxA"
                     /locus_tag="AC16PP_06780"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR6481368.1"
                     /translation="MPISLAITPGEPAGVGPELLLRLAESPRTRPWVAVADADLLAER
                     ARALGTTVRLDDAWDRPAMEAGRLTVRHVPLEAPCTPGQLDPRNAAYVLETLRLATDG
                     CLSGEFAGLVTGPVQKSVMNDAGIPFTGHTEFLCERAGVADVLMLLVAGTLRVGLATT
                     HLPLREVPDALSRALLEGRLGLLLDGLRRDFGIARPRVLVAGLNPHAGEGGHLGTEEI
                     EIIDPVCERYRAAGEQVKGPLPADTLFTPAMLDTADAVLAMYHDQGLPVLKHAGFGRA
                     VNVTLGLPFVRTSVDHGTALDLAGSGVADPGSFAAAASLAGDLLDFRASA"
     gene            complement(8512..9789)
                     /locus_tag="AC16PP_06785"
     CDS             complement(8512..9789)
                     /locus_tag="AC16PP_06785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6481369.1"
                     /translation="MSLTLGLLTASALASAPALAEYRELDAIVAVVDEDVVLASELLS
                     RLKSIREQFAEQQGQLPSDDILVSQLMERLIIESLQMQEAERRGVQIDDETLTRAVMS
                     FAERNGMSAEQFRQALAADGVSYVEFREQIKQEMTLQRLQRNLVNRRITITDSDIDDL
                     LNSPYYQQLLSDEFRVGHILLAIDDKATEEAIQEAARQAVEIVEELRAGADFAQIAVA
                     RSAGSRALEGGDLGWRRAGELPSLFADQVLALEPGETAEPVRSASGIHIVQLLEQRGA
                     GMQKEEQTLLRHILVMPSEIRSEEEAEALIRQVHDELEAGGDFVALAAEHSEDPGSAL
                     NGGDLGWTSGNEFVPAFREAMYATTTGELSEPFRSQYGWHVLEVQERREQDLSEETRR
                     NMAMQILHQRRFDEELQEWLKELRDEAFVEMRL"
     gene            complement(9822..>12067)
                     /locus_tag="AC16PP_06790"
     CDS             complement(9822..>12067)
                     /locus_tag="AC16PP_06790"
                     /inference="COORDINATES: protein motif:HMM:NF016348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HIR6481370.1"
                     /translation="RLPSYCDGAYLQPAFPYPRSVDDEDYPLHAEARLGEYWTETDGK
                     VTLSGAVVMRQGNRTLATERATLDRATNEVTLEGGVSILEPEIALGGAGARTNIDTNA
                     AELEDADYLLLDADMRGVAEYLERDEQGTLRVKRGTFTRCEPGNNTWRISSGSLKVKE
                     GAIFASARNATLRVKGVPIAYTPYIRFPVKDDRQSGFLFPNIEYSGEEGLDLTLPYYF
                     NLAPNYDATVVPRYIANRGLNLETEFRHLNRWQGTTLSGAILPKDDLYDGTYRKKDFE
                     DLVEAGELAGEFEPANRWLYGMQHRGRLGRFRSYVDYTAVSDRDYFRDLGSDLGISSQ
                     IDLERRGELQYNSGGLFARVWAQRFQRLDEVTVDPYQRLPEVDVTYTGDLAGRLEYSL
                     GAQWVSFTRDNEDLGGFGAVVGDRLHLEPRVRVPFSWPWGFLNFSGGYRYTKYDLEDV
                     PVGVEEKPDRGIGLGSADAGLYFDRDLELFGTELVQTLEPRVFYLYQEYEDQSTLPNF
                     DVSKLTFGYSQLYRDNRFSGLDRIGDSNQLSIGLTTRFIDGANGREYLRASIGQIVYF
                     RDRLVTLGGPTDDDQDGSSALAGELSFALARAWRLTGTLVWDVKDNQIDEAAAALTYR
                     RDSRHMFNLGYRKRLESNIHQTDAALYWPLGRRFGLIGRWNYDIESGRTIEGLAGIEY
                     NNCCWQLRLVARRYIDSPSARQIATVDADKGIFFQFVFKGLAGFGDRLEAVLRRGIRG
                     YSTEVYD"
BASE COUNT         2093 a         3907 c         3865 g         2202 t
ORIGIN      
        1 cccacggcgt cgccccgcca gcgcagttgc cgctcgttcg gccgaggatc gggtaggcgt
       61 ctaccagggt tcccgactca tcgaaccgca gggcgctggc accgccgaac cccagcggca
      121 tctcgcagtt gctgacgtag acccaacctc cttcgggatg cgcaaacgta gcgcctccat
      181 ccggggccgc gtgccaggcg taggacgagg acgacacggg gcgattgccg gactcggcaa
      241 cgatcctcga gctgaacccg gcaggcagcc ggataccgtt gtgatccggg gcaagcagct
      301 cgcccaggcg gcccatcgcg gacccagggc ccagtctgtc cgcccaattg gcagacgacg
      361 acccaccttg ggtagggcag gcgcgagcga gcgagggcct cagcagatac gcggtgccga
      421 taccggcaat ggtcaggaaa gatcgtctgt ccatctcggc attgtaacag gggctacgcc
      481 gccgactgag cctcctccgc ggggcaggcg gtggtggcaa ccacctcgtc gtgccagacg
      541 gactccaggc gaacgggaaa tccccagagc cgatgcaggt gccgcatgac ctcgtcggtg
      601 ctttgaccca tgggctggcg cgcatgctgc gcgtgccgca gcgtgaggct ccggtcaccc
      661 ctgacgtcga cccgcaccac ctgaatgttc ggctcccggt cgccaatgtt gtactgcccg
      721 gccagaaggc tgcgcacctc gcggtagccg ccccgatcgt gaatggccga gatgagcacc
      781 tcgtcttcgg cgtcgtcgtc gacgacgccg aacagacgca tctcgcgcat gaccctgggg
      841 cttaagaact gcagcacgaa gctctcgtcc ttgaagtccc gcatggcggc cggcagcacc
      901 tcgagccagt tgcccccggc gatgtcggga aaccactcgc ggtcctcgtc cgtcggatgc
      961 tcgcacacgc gccgcagatc cgagtagatg gcaaagccca gcgcgtaggg attgatgccc
     1021 cggtacgcgg ggtgatcaaa actcggctgg aacagaaccg ctgtgtgcga ggcgagaaac
     1081 tccatcatga agccgtcggt gaccaggccg cgatcgtaga gctcgttcaa aagggtgtaa
     1141 tgccagaacg acgcccaccc ctcgttcatc acctgggtct gccgctgagg gtagaagtac
     1201 tgcgccacct tccggacgat gcgcacgatc tcgcgctgcc agggctccaa gagcggcgcg
     1261 tgcttctcga tgaagtagag gagattttcc tgaggctccc gcgggaagac ttcctcgtcg
     1321 ctcgagtcga gctgccgggc ctggggaagc gtccgccaca gatcgttgag cgtgcgctgg
     1381 acgtattcgg cgcgttcggc ctggcgccgc ttctcttcct ctgccgagat cgggtacggc
     1441 cgcttgtagc ggtcgacccc gtagttcatc agggcgtgac aggagtcgag gatcatctcc
     1501 acttcctgca cgccgtagcg ctcctcgcag ctggcaatgt agttacgggc gaagagcagg
     1561 tagtcgacga tggcgctggc gtccgtccag gtctggaaca ggtagttccc cttgaagaaa
     1621 gagttgtggc catagcaggc atgggcgatg accagggcct gcatggtcat gctgttctct
     1681 tccatgaggt aggaaatgca gggattcgag ttgatcacga tctcgtaggc caggcccatg
     1741 aatccgcgct ggtagctgcg ctcgacgtcg acgaagtgct tgccgaagga ccagtggtga
     1801 tagccgatgg gcatgccgat ggaggcatag gcatccatca tctggtccga acggatgatc
     1861 tcgatctgat tggggtacgt gtcgagaccg aactgctcgg caatcgcgcc gatctcgcgg
     1921 tcgtaccgac cgatgagctc gaacgtccag tccccgccgt cggagatggg ctcgcgcggc
     1981 cccctgcccg gcaagaggat ttccatcacg cggcgtcctt cttctggaag aggtcgtgaa
     2041 acacgggaaa gatgtccgac acgtcggtaa tctggcgctg ggcaaacgat tccggaaacc
     2101 gctcggccag gtgctcgtac tcccgccaca gcgcctgatg gctgcgcttg gtgatctcca
     2161 cgtaggagaa gtactgcacc agaggcagga tcttcttctc cagaagctgc cggcagaggg
     2221 gcgagtcgtc gttccagttg tccccgtccg aggcctgggc cccgtaaatg ttccactcgt
     2281 tgggcgagta ccgatcttcc agcacctcga gcatcagctt gagcgcgctg gagaccacgg
     2341 tcccgcccgt ttccctggaa tagaagaact cctgctcgtc cacctccttg gcaacggtgt
     2401 gatgccggat gaagacgatc tccgtgcgtt cgtaatggcg cttcaagaac atgtagagca
     2461 acaggtagaa gcgcttcgcc atgtccttga tgtgctggtc catggagccg gacacgtcca
     2521 tgaggcagaa catcacggcc tgggaggtgg gcaccggctt gcgcaagtgc aggttgtagc
     2581 gcagatcgtt ggtatccaga aagggtatgc ggccgagccg tttttccaag cgctcgatct
     2641 cgtcgcgcat gcggcgacgc tggagctcat cgccgtcgag ctccgcctga tcgaacgcct
     2701 cgcgcgcctc gcgaagcagc cgcttgggct cgccggccag cgcgatgcgc cgcgccttcg
     2761 cctgccgcag ggaacgcagc accgagagct tggcgggcac cccgtcgctg gtgaacccgg
     2821 catgttcgta cttgaagctc gagttgcctt tcagatgccg cttggtgagg ttgggcaggg
     2881 ccaggtcgtc gaagaggtac tcgaggaact cctcctgggt gagctgaaag acgaagtcgt
     2941 cggaaccctc accagagttt gaggcctcgc catccccgga accgccgccg gcgccgcctt
     3001 ccggacgcgc aatgcggtct ccggcaatga actccttgtt gccgggatgc acgatgctgc
     3061 ggcgcccgcc gctgccgtgc tgaaagaccg gttcggagat gtcctgggcg gggatggtga
     3121 cttcctcgcc ccgttccatg tcggtgatgc tgcgctcgtt caccgcatcc gataccgcct
     3181 tcttgatgcg ctgcttgtaa cgctccagaa aacgctgccg attgacggcg ctcttgttct
     3241 tggcgttttc ccggcggtcg atgatgtagc tcatgccatg cccccgctgc gcggtgcctc
     3301 tcaccgtccc gggggcatca gcctccggga cggtgagagg gttccgttgc tactgtgact
     3361 tgcgaactct caggtaccac tccgcgagca gtcgcacctg cttgtcggta taaccgcgct
     3421 ccaccatccg ggcgacgaag tcgtcgtgct tgcgctgctc gtcgctggag gacttcgcgt
     3481 tgaaagagat caccggcaga agatcctcgg tgttggagaa catcttcttc tctatgaccg
     3541 tgcgcagctt ctcgtagctc aaccacgacg ggttggtgcc gttgttgctt gcacgcgccc
     3601 tcagcacgaa gttgacgatc tcgttgcgga agtctttcgg attgctgatg ccggcgggct
     3661 tctcgatttt ctccagctcc tcgttcaggg aagcgcgatc gagaatgtcg ccggtttccg
     3721 ggtcgcgata ctcctgatcc tgaatccaga aatcggcgta tgtgacgtag cggtcgaaga
     3781 cgttctgacc gtactcggag taggactcga ggtacgccgt ctggatttcc ttgccgatga
     3841 agtccacgta cttgggagcc aggtactcct tgatgaagcg cagataccgg tcccggacct
     3901 cctgaggata ctgctcctgc tcgatctgct gctcgagcac gtacatcagg tgaacggggt
     3961 tggcggccac ctcgatggcg tcgaaattga acaccttcga aaggatcttg aaggcgaagc
     4021 gggtggacag accgtccatg ccctcatcga caccggccgt gtcgcggtac tcctgaagcg
     4081 tcttggcctt ggggtccgta tccttgaggt tttccccgtc gtagattcgc atcttggaga
     4141 acacgttgga gttctccggc tccttgagcc gggaaagcac cgagaactgc gccagcatgc
     4201 gcagcgtgtc gggggcgcag ggcgccgcgt tcagagagct ggtatcgatc agcttccggt
     4261 agatgttcac ttcctcccgg acccgcaggc agtagggcac cttcacgatg tacacgcggt
     4321 cgatgaaggc ttcgttgttg cggttgttac ggaacgtctg ccactccgac tcgttggagt
     4381 gggccagcac gatcccgtcg aacggaatgg agccgatggc ttcggtgccg ttgtagttgg
     4441 cttcctgggt cgcggtgagc agcgggtgca gcaccttgat gggcgccttg aacatctcga
     4501 cgaactcgag catgccctga ttggccttgc acagcccgcc ggagaagctg tacgcgtccg
     4561 ggtcgttctg cgcgaagtcc tcgagcttgc ggatgtccac cttgcccacc agggcagaga
     4621 tgtcctggtt gttctcatca ccaggctcgg tcttcgacaa ggccacctgg tccaggatcg
     4681 acgggtattt cttcaccacc cggaactggg tgatgtcgcc gccgaattcc ttgagccgct
     4741 tggccgccca tggcgacatg agtccccgaa cgtagcggcg cgggatgccg tagtcggact
     4801 cgaggatggc gccgtcctca acgggatcga acagccccag aggcgactcg ttcaccggcg
     4861 agcccttcag cgcgtagaac ggttcgcgct gcatgagcgc tttgagcttt tcggcgagcg
     4921 agctcttgcc gccgccgacg ggcccgagga ggtagagaat ctgctttttc tcctcgaggc
     4981 cttgggccgc atggcggaag aaagacacga tctgctcgat ggcttcttcc atgccgtaga
     5041 agtcgtcgaa cgccgggtag cgcttgatga tcttgttgga aaagatgcgc gaaagccgcg
     5101 aatccttggc ggtgtcgaca aactccggct cgccgatcgc cagcagcagc cgctcggcgg
     5161 ccgtggcgta ggtggcgggt tgttctttac agagatccag gtattcctga agggaaagct
     5221 cttcttcgcg agccgcttcg aatcgttcct gatagtgctc gaatacaccc atttttgcta
     5281 gcgctccttt gctgcatccg cgtgtacctc actgaagcta ccgttgcatt cagcgtcgat
     5341 ccgtgaaccg tgtcacacac gcacctgcga ccggaatcac taatggatga gcggctaatt
     5401 ccgccttatc ggcggtacgg aacgcgtgat tcgttgacat ttggaacggc gaaacccaag
     5461 gaatgggact caagtcgcct ctgaaccgat ccgtgctgcg ccggagttgc cggaccgggc
     5521 aagaaaacgg ctttgttacg gcccgcccgg ggcccctgtc acctcagccg cccgcccgaa
     5581 ccgaatacgt ctcgcccgtg tccaggcaca gggcgctgag cttgcggccc cacacgcatc
     5641 ccgtatccag ggcgatcacg tccttcagcc ccgtgtgccc ctgcagcgcc gcccagtgac
     5701 cgaaaaccag cttgaccggc agcggtttgc ggttgcgcag ctcgtaccac ggcagccagg
     5761 gcttgggcgc gtcggccagc ccccctttga aggaaaaatc caaccgtccc gccttgtcca
     5821 ccagacgcat gcgggtgaag tagttggtga tgcagcgcag ccggtccatg cctgcgagct
     5881 tcggatccca cacgtccggc acgttgccgt acatgctttc gaagtagcgt ttgcgccgtt
     5941 tgccgcgaat ggctttttcc acctcgccgg ccagagcttc cgcgacatcg atgtcccaga
     6001 tatgaggaat accggcatgg gtcatgacgt agcccagctt gttactccgg accagaagcg
     6061 gctgctggca cagccactcg gcgatctcgg cgcgggccgg ggagctcagc agatcatcga
     6121 acgtgtcggt ccggttggcg ctgtagccgc cgtaatggat ggcgagaaaa tggagctcgt
     6181 ggttgccgag cacgatgacc gcgcgcttgc ccagggaacg caccaggttc agaacccgca
     6241 acgaatcggg cccacggttg atcagatcac ccaccatcca gagccgatcg cgcttcttgt
     6301 cgaactccat tttcttgagc agccggcaga actcggcgta gcaaccctga acgtcaccga
     6361 tcgcgtaggt ggccactgcc cggtctcaat gcaccgcgtt gggcgcggct agcgagaaca
     6421 tgggaatcgg cacctcgaag cgcgtgccgt cgtcacggcg gaagccgtag agcccgtgca
     6481 tggttcccac gggcgtttct aaaaccgcgg cgctggtata acggaaggcg gtgccgggct
     6541 cgagccgggg ttgctcgccg accactcccg gcccctgcac ctcgcgcacc tcgttgtcgc
     6601 cgtcggcgat gaaccagtgg cggtcgagga gctgaccggg ctccggcccg aggttgctga
     6661 tcgtaatggt gtaggcgaac acgtagcggt tccggatcgg atccgactcg ccgacgacgt
     6721 agcgcgcatc caccgccacc tcgatgccgc tggcgggtcc gctcacggtg tctgctccaa
     6781 ctgcacggcg ttggcgagcg tgacgaaatc cttcaccgac agctggtcgg gccgggcggc
     6841 gtcatccaca tcgagttcat gccagggcat gctcaaggat tttagtgcat tgccgagccg
     6901 tttgcgccgc ccggagaacg cgtggcgcag aaccagatcc agcaggtccg ggttctcgag
     6961 cgggagcttg gccgcatggg gcaccagccg cgcgacggcg gaccagactt tcggcggtgg
     7021 gctgaaactc tccggcgcaa cctcgaagag cagctccacc tggcagtggt actgggcgag
     7081 caccgtcagc cggccccatg ccttggtgcc cggaacggcc gcgaggcgca gcgccacctc
     7141 gcgctggagc atgaagtgca tgtcgcggat tcggtcggtg caggcgaaca gacgaaccag
     7201 aagcggcgtg gagatgttgt agggcagatt gcccaccacg cgccagctct gcgagcccag
     7261 cagctcgtcg agcggaacgc gcagcacgtc ggcgttgacg acctccacgg tggggaagcg
     7321 gcgcccgagc tcaggggcca gatcgcggtc gatctccacc gccaggtaac gcgctggcgc
     7381 cgccccggag gcgtcgagca gagcggcggt gagcgccccg gtgcccggac cgatctccag
     7441 cacctggtcg tgcggcccga gacggatcag atccgccatg cgctgcagca ccgcttcgtc
     7501 ggtgagaaag tgctggccga accgcttacg cgctcgcacg gaaatccagc aggtcgcccg
     7561 ccagcgaggc ggcggcggca aagctgcccg ggtcagccac cccggagccc gccaggtcaa
     7621 gcgcggtacc gtggtcgacc gacgtccgca cgaagggaag gccgagcgtg acgttgaccg
     7681 cccgcccgaa cccggcgtgc ttgagcaccg gaagaccctg gtcgtgatac atggcgagca
     7741 ccgcatctgc ggtgtcgagc atggccgggg tgaagagggt gtcggcaggc agcggcccct
     7801 tcacctgctc cccggcggcg cggtagcgct cgcacacggg atcgatgatc tcgatctcct
     7861 cggtacccag gtgcccgccc tcccccgcgt gggggttcaa accggcgacc agtacccggg
     7921 gtcgggcaat accgaaatcc cttcgcagcc cgtcaagcag caggccgagc cggccctcga
     7981 gcagagctcg ggaaagggcg tcgggcacct cgcgcagggg caaatgggtc gtggcgagcc
     8041 cgacccgaag cgtgcccgcc accagcagca tgagcacgtc ggcaacgccc gcccgctcgc
     8101 acaggaactc cgtgtgcccg gtgaacggga tgcccgcgtc gttcattacc gacttctgca
     8161 cgggtcccgt gacaaggccg gcaaactccc ccgacaggca accgtccgtc gccagccgca
     8221 aggtttccag gacgtaggcg gcgttgcggg gatcgagttg cccgggggtg caaggagcct
     8281 cgagaggcac gtgccgcacg gtcagccgcc cggcttccat ggccgggcgg tcccaggcgt
     8341 cgtctaaccg gactgtggtg cccagggcgc gggcccgctc ggccagaaga tccgcgtcgg
     8401 ccaccgctac ccaggggcgc gtgcggggac tttccgccag tcggagcaga agctccgggc
     8461 cgacgcccgc cggctcgccg ggcgtgatgg cgaggcttat cggcacaccc gctagagcct
     8521 catctccacg aacgcttcgt cccgcagttc cttcagccac tcctggagtt cctcgtcgaa
     8581 gcggcgctga tggaggatct gcatcgccat gttgcggcgg gtctcttcgc tcaaatcctg
     8641 ctcgcgtcgc tcctgaacct ccagaacgtg ccagccgtac tgggagcgaa acggttcgga
     8701 cagctcgccc gtcgtggtcg catacatcgc ctcgcggaac gcgggcacga actcgttgcc
     8761 cgacgtccag cccaggtcac cgccgttgag cgcgctgccc ggatcctccg agtgctcggc
     8821 cgccagggcg acgaaatcgc cgccggcctc gagctcgtcg tgaacctggc ggatgagcgc
     8881 ttcggcttcc tcttccgagc ggatctccga gggcatgacc aggatgtgac gcagaagcgt
     8941 ctgctcttct ttctgcatgc ccgcgccccg ctgctcgagc agctggacga tgtgtatgcc
     9001 gctggcgctg cggacgggct cggcggtctc gccgggctcg agtgccagga cctgatcggc
     9061 aaagaggctc ggcagctctc cggctctccg ccagccgaga tcgccgcctt ccagcgcccg
     9121 actccccgcc gaccgggcca cggcgatctg agcgaagtcc gcgcccgcac gaagctcctc
     9181 gacgatttct accgcttgcc tggccgcctc ctggatggct tcctcggtcg ctttgtcgtc
     9241 gatggccagc agaatgtgcc cgaccctgaa ctcgtcggaa agcagctgct ggtagtaggg
     9301 cgagttcagc agatcgtcga tgtcgctgtc ggtgatggtg atgcgacggt tcaccaggtt
     9361 gcgctgcagg cgctgcaggg tcatctcctg cttgatctgc tcgcgaaact ccacgtagct
     9421 gaccccgtcg gcggccaatg cctgacggaa ctgctccgcg ctcatcccgt tgcgctctgc
     9481 aaacgacatc accgccctgg tcagggtctc gtcgtcgatc tgcacgccgc ggcgctcggc
     9541 ctcctgcatc tgcaggctct cgatgatgag ccgctccatg agctggctga ccagaatgtc
     9601 gtccgacggc agctgaccct gctgctcggc aaactgctcg cggatgcttt tcaggcgcga
     9661 aagcagctcc gaggcgagca ccacgtcctc gtccaccacc gccacgatgg cgtcgagttc
     9721 ccggtactcg gccaacgccg gcgcgctcgc aagcgcagag gccgtcagca acccgagcgt
     9781 caggctcatc acccgtcgcg atccgcttcc caaaccaacc atcagtcgta aacctccgtg
     9841 ctgtatcccc ggatgccgcg gcgcagcacc gcctcgagcc gatcgccgaa ccccgccagg
     9901 cccttgaaga cgaactggaa gaagatgcct ttgtccgcgt ccaccgtggc gatctgccgc
     9961 gcgctggggc tgtcgatgta acgtcgtgcc accagcctca gctgccagca gcagttgttg
    10021 tactcgattc ccgccaggcc ctcaatcgtt cgaccgctct cgatgtcgta gttccaccgg
    10081 ccgatcaggc cgaagcgacg tccgagtggc cagtagaggg cggcatcggt ctggtgaatg
    10141 ttgctctcca ggcgtttgcg gtagcccagg ttgaacatgt gccggctgtc gcgccggtag
    10201 gtgagcgctg cggcggcctc gtcgatctgg ttgtccttga cgtcccagac cagggtcccg
    10261 gtgaggcgcc aagcgcgggc aagggcaaac gacagctccc cggccagggc ggaggagccg
    10321 tcctgatcgt cgtcggtggg gccaccgagg gtcacgagcc gatcccggaa gtagacgatc
    10381 tggccgatgc tggctctgag gtactcgcgc ccgttggcgc cgtcgatgaa gcgggtggtg
    10441 aggccgatgg agagctggtt ggaatcgcca atccggtcga ggcccgaaaa gcggttgtct
    10501 cgatacagct ggctgtaacc gaaggtgagc ttgctcacgt cgaagttagg cagcgtgctc
    10561 tgatcctcgt attcctggta cagatagaac accctgggct ccagggtctg caccagctcc
    10621 gtgccgaaga gctcgaggtc ccggtcgaaa tagagccccg cgtcggcgct gccgaggccg
    10681 atgccacggt cgggtttctc ttccacgccc accggcacgt cctcgagatc gtatttcgtg
    10741 taacggtagc cgccgctgaa gttaaggaag ccccagggcc agctgaaggg cacgcgtacc
    10801 cgcggctcca gatgcagccg gtccccaacc accgccccga acccgccgag atcctcgttg
    10861 tcccgggtaa aagacaccca ttgggcaccc agcgagtact ccaggcgacc ggccagatcg
    10921 ccggtgtagg tcacgtccac ctcgggcaga cgctggtacg gatccacggt gacctcgtcc
    10981 aggcgctgaa accgctgcgc ccagacccgg gcaaagaggc cgcctgagtt gtactgcagc
    11041 tcgccgcgcc gctcgaggtc gatctggctg gatatgccca ggtccgagcc gagatcgcga
    11101 aagtaatcgc gatcgctgac tgcggtgtag tccacgtagg agcggaagcg gccgagccgg
    11161 ccccgatgct gcatcccgta tagccagcga ttggccggct cgaactcgcc ggcgagctca
    11221 ccggcctcca ccagatcctc gaagtccttc ttgcggtagg tgccgtcgta gaggtcgtcc
    11281 ttcggcagga tggcgccgga aagcgtcgtg ccctgccagc ggttcaagtg ccggaactcg
    11341 gtttcgaggt tcagcccgcg attggcgatg tagcgcggca ccacggtggc atcgtagttg
    11401 ggggcgagat tgaagtagta gggcagcgtc agatccagtc cttcctcccc cgagtactct
    11461 atgttgggaa acagaaagcc cgactggcgg tcgtccttga ccggaaagcg gatgtaaggc
    11521 gtgtaggcaa tgggcacgcc cttcacgcgc agcgtggcgt tgcgggcaga tgcgaatatc
    11581 gcgccttcct tgaccttcag gctgcccgag gagatgcgcc aggtgttgtt gccgggctcg
    11641 cagcgggtga aggtgccacg cttcaccctc agggtgccct gctcgtcgcg ctccagatac
    11701 tcggcgacac cccgcatgtc cgcatccagc agcaggtagt cggcgtcttc cagctccgcc
    11761 gcgttggtgt cgatgttggt tcgtgcgccc gccccgccca gcgcgatctc gggctccaag
    11821 atgctcaccc cgccttccag cgtgacctcg ttggtggccc ggtccagggt cgcgcgctca
    11881 gtcgccagcg tccggttgcc ctgccgcatc accacggccc cgctcaacgt gaccttgccg
    11941 tcggtctcgg tccagtactc gccgaggcgg gcctcggcat gcaggggata gtcctcgtca
    12001 tcaacggagc ggggataggg aaaggccggc tgcagatatg cgccgtcgca gtaagaaggc
    12061 aatcggg
//
LOCUS       DBNHKV010000213        13063 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1022388, whole genome shotgun sequence.
ACCESSION   DBNHKV010000213 DBNHKV010000000
VERSION     DBNHKV010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13063)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13063)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13063
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1022388"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="AC16PP_06795"
     CDS             complement(<1..271)
                     /locus_tag="AC16PP_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582840.1"
                     /note="cytochrome c-type biogenesis protein; required for
                     the transfer of heme to apocytochrome c; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfE"
                     /protein_id="HIR6481371.1"
                     /translation="MIVELGHLALVIALCLSGLLAVFGLGGLNLAGGRLLRANLSLVT
                     GQAVFCALAFGVLTQAFLADDFSVAYVANNSNTLLPWYYKFSAVWG"
     gene            complement(268..696)
                     /gene="ccmE"
                     /locus_tag="AC16PP_06800"
     CDS             complement(268..696)
                     /gene="ccmE"
                     /locus_tag="AC16PP_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017849438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HIR6481372.1"
                     /translation="MNPKRRNRLIVVSFLLLGTMATAFFVLVALNENLNLFYPPDQIV
                     AGEAPLGTQIRAGGMVLEGSVARTGNELDVEFVLTDYQGAAFTVLYSGILPDLFREGQ
                     GILVRGELDPSGVFRAEEVLAKHDENYMPPELDGMHAGPP"
     gene            complement(693..884)
                     /gene="ccmD"
                     /locus_tag="AC16PP_06805"
     CDS             complement(693..884)
                     /gene="ccmD"
                     /locus_tag="AC16PP_06805"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HIR6481373.1"
                     /translation="MSFETFGDFLTMGGHGPYVWLSYGLTLAVVLVNVLAPITGRRRY
                     LREQADVERRRQAAEGVRT"
     gene            complement(881..1624)
                     /locus_tag="AC16PP_06810"
     CDS             complement(881..1624)
                     /locus_tag="AC16PP_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HIR6481374.1"
                     /translation="MKEFWHRLGSPRWFYGISGPWLKILAGLAALLLGTGCIWGLAFA
                     PPDYQQGNSFRIIYVHVPTAIVAQTAYLVMGCAGVVLLVWRMKLADMVLATVAPFGMW
                     MTVLALMTGAIWGKPTWGAWWVWDARTTSMLVLLFLYFGIYALRQAIPRQEAAGRACA
                     VLAVIGVVNIPIIKYSVDWWLTLHQPATFKLTAAPSMPVEMWLPLAVNVVGIYCLFGA
                     NLLSRLRLEILQREAGTQWVRDMVLEEAR"
     gene            complement(1749..2420)
                     /gene="ccmB"
                     /locus_tag="AC16PP_06815"
     CDS             complement(1749..2420)
                     /gene="ccmB"
                     /locus_tag="AC16PP_06815"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005740276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIR6481375.1"
                     /translation="MAGLFRAQLRRELQTTARSVGVAVNPLVFLFLAMTLFALGAGGG
                     PDDLREHAPGIIWVLVLLASMLSLEALFRSDYDDGTLEQMLLLAEPLFVPLLAKITAQ
                     WCVSGLAMTLLAPLAALILSLPAASVPTLAATLLLGTPALSLLGAVGAALTVGLRRGG
                     VLLGLLILPLYVPVLIFGAGAVSADMAGTGAAGQLYWLAAITMLALTTAPFGVQAALK
                     ISVEQ"
     gene            complement(2444..3007)
                     /gene="ccmA"
                     /locus_tag="AC16PP_06820"
     CDS             complement(2444..3007)
                     /gene="ccmA"
                     /locus_tag="AC16PP_06820"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009621121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HIR6481376.1"
                     /translation="MLRVVDAGCRRGGRQLFTELSLELGAGECVELHGPNGSGKSTLL
                     RCIAGLYPDFEGVVETAEFLYAGHRLGLNPLLSAEQNLAWYAALRGTADAVLPALERV
                     GMAGYERTACQHLSAGQQRRVALARLLVCRSPLWLLDEPLTALDGPGQELAVTLVTAH
                     MDGGGAVVAATHQPLGLERSRLLSLGR"
     gene            complement(3074..4462)
                     /gene="ppnN"
                     /locus_tag="AC16PP_06825"
     CDS             complement(3074..4462)
                     /gene="ppnN"
                     /locus_tag="AC16PP_06825"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008373414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HIR6481377.1"
                     /translation="MNTTTGQIRKVDVHVSPRGSLELLSQLEVNALASTAAESPLLDL
                     FRRCALAVLNTGSESDDAAAIFREYADFSIEVARRTRGVKLIIKNAPASAFVDGRMIE
                     GVRQHLFSVLRDIVYIGTDIADSDLFDLNSSEGITDAIFHILKNARVLRPDLPPRTVV
                     CWGGHSISRTEYEYTKEVGYHLGLRGLDICTGCGPGAMKGPMKGAAVGHAKQRQQDAR
                     YVGLTEPGIIAAEPPNPMVSDLVILPDIEKRLEAFLRIGHGVLVFPGGAGTVEEILYL
                     LGVLLDPANEGEEMPLIFTGPAESADYFFELDAFLRLSLGDEVARFYDIVIGDAEEVG
                     TRIGRRVRRVRRHRRQVGDAYYFNWLLNVPLAHQQPFKVTHENVAALRLSADLPAHEL
                     VVNLRRAFSAIVTGNVKDDGIRMIESHGPFELHSDRHLIGALEQLLRSFSENGRMKLI
                     GPYEPCYRVAAG"
     gene            4687..6783
                     /locus_tag="AC16PP_06830"
     CDS             4687..6783
                     /locus_tag="AC16PP_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor family protein"
                     /protein_id="HIR6481378.1"
                     /translation="MSGSSMIRALLAVAALCSATGAATGNEPTDTAGPALEELVVSAT
                     RTNRTVREVPAAVSVVGREDIQLGRQQLGLDEALGRVPGLFSQNRYNFAQDLRLSIRG
                     FGARANFGIRGLKLIADGIPATTADGQSGVDDIDLGSLDRIEVIRGPSSSLYGASSGG
                     VINLMTEDGPERPFVEAGVTLGEYDQQRYQLKAGGQTGRLNYLASASHLAYDGYREHS
                     RVESTIFNSKFRYGIDSASNLTVIVNLVDSPTADDAGAITQAQVEDDPRQAQQRNLSS
                     NAGESIEQQKLGIIYDRALDDRNAITLRNYYVWRDFQAFLPIGTHIPFVADDGVVEFD
                     RFFWGGGIQWRFADSLFGRPFQMSAGVDADIQEDDRQRYLNEAGVKGALAFDQLEKAE
                     AYGAYLRGEIGLADSVRLALGIRWDTVDLSVDDSFLDNADQSADLDFDELSPSVGLVW
                     DLGENWSAYANYATAFETPTFTELAVPARDLDVSLGGFANVSAQEAQSFEAGLRGGLL
                     DGALYVEAAAFTMEVDDEITSVSNIGNRSFFANADTDRRGIETFANLAFEGGLELSLA
                     YTYSDFEFDRFEDNEDFEGNALPGLPEHQLFAEIKYRHGSGAYAVADVLHVSKLYVNN
                     ANSGTSEASTVANLRVGASLALGQWQLDPFLGVNNLFDENYISNVRINGFGGRLFEPA
                     PKRNIYGGVAVRYVLR"
     gene            complement(6802..7032)
                     /gene="nrtS"
                     /locus_tag="AC16PP_06835"
     CDS             complement(6802..7032)
                     /gene="nrtS"
                     /locus_tag="AC16PP_06835"
                     /inference="COORDINATES: protein motif:HMM:NF038050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NrtS"
                     /protein_id="HIR6481379.1"
                     /translation="MSTQHGFLRLAARPDVVARAARTALLVGVILIAINHGDALLSGD
                     VGPVQAGKMALTVLVPYCVSTYASVRALQSAG"
     gene            complement(7029..7526)
                     /locus_tag="AC16PP_06840"
     CDS             complement(7029..7526)
                     /locus_tag="AC16PP_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481380.1"
                     /translation="MLRRNTVWLVLVATGLLASTAYPPEAAARVTLSNTVARYEIAQG
                     LDGQVERRLVAADEVLPGDELLYTITFANEGREEVAAGSIVITNPIPEVAEYVEGSAA
                     GEDTFITFSVDGETFAEPSGLMVQRGDGQRLAEPSEYASIRWAYQQALEPGESGAVSF
                     ALRLR"
     gene            complement(7619..7864)
                     /locus_tag="AC16PP_06845"
     CDS             complement(7619..7864)
                     /locus_tag="AC16PP_06845"
                     /inference="COORDINATES: protein motif:HMM:NF018500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HIR6481381.1"
                     /translation="MGCGSGHENDGAAIKVPLSELSEEALAGVVESFVLREGTDYGHR
                     EFTLAEKRALVRKQLESGDAVIYFDPLTRSIDIKPAR"
     gene            7952..8659
                     /locus_tag="AC16PP_06850"
     CDS             7952..8659
                     /locus_tag="AC16PP_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008093405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6481382.1"
                     /translation="MAEPDHILVVEDEPITREQLVSYFEEEGFRVTSTGSGDEVHELV
                     KNSDIILVLLDIKLPGKDGLTLTREIRAQSDVGIILVTSKQEQIDRILGLESGADDYV
                     TKPFDPRELLSRARNLIRRVQLQQRQRRKNHIRNFEGWQLDLNKRELTSPEGEKTTLS
                     AGEYQLLLAFMEQAGEVMNRDQLMNRIRNREWYPDDRYIDVLVGQLRKKLGERAANAK
                     IIATIHGTGYLFTPEVA"
     gene            8726..>13063
                     /locus_tag="AC16PP_06855"
     CDS             8726..>13063
                     /locus_tag="AC16PP_06855"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6481383.1"
                     /translation="MSSVDEKIYESASTVVRRTTWQDQPVVAKRLKPEGLPSNVLSQF
                     HHEFHLNQSLTSPNVCRALAMDDSGLEIYFEDTQGASLRSLIDTGELSLDERIAIAVE
                     LSRALQSVHDEGVIHRDVNPGNVLVSEDPMRVHLIDFGLATLAAREYPEVEPLGQVTG
                     TLPYLSPEQTGRVNRVVDYRTDLYSLGATLYELLAGNPPFTNTDPLELIHAHIASTPR
                     PLTAIGEGIPRWLSEIVQKLLAKQPEDRYQSAAAVRDDLIRGQTQANVVPFRLGQTDS
                     PGLLAMPKRLYGRTAQIGIIQEYLERAARGEVLFLQITGAQGRGKSSLCAELARQAAG
                     TKAMVARTNALAFDFRDTDAVWIELTRQLLRQALADAKAGDLLARIAALNSPNVRSLT
                     ELIPELRDLVPAGSEGAGLPGVGIQEVLKALQPQPLCLIVEEVDNVPSESLETLLSMA
                     LDSRHLLMTVTRANADSDAFRTPRLATKAATLELPAFARADVRSLLSDMLSTSEVRVR
                     ELASELHAKTDGLPADVLQLIEELHHLGHIFRDANAAEWEWDMAAIRAHYFSNNSADR
                     IRRLISELSEETTHALQVGAAVGEQFSSELAATLLDVEQAELAGRLRPALTKGLLTTA
                     TGSKEAPGDQHQLLYRFAHPRVRSLVYAEMAQEAKSTIHFAIADECKRGHRPAGRTLL
                     QIAHHLNAAADPIGLDKDKRLEIAHYNLLAAKESIGQGAFQPAYKFCRSGLSLLSELP
                     DEPIFIELCQSAAEAAFYCSDFEQLDRVIASIPHNTSALDEIRVRAAVVQNHLGAARE
                     QAFDALESLGYVRPATGINPIGQAFRRLGRKRAPDAAPKPAPVLEDIRLQHTFRLLGY
                     LLHAGYHLGSEGLDALTDDMLSEAARHGYSAEIAFAFAARAVSAVADHRIGIAQRYAG
                     EARALAGQFSGDAFSIRAETLLNGLVDPWSGGLDDTLGGLSRNIDASMACQDYEFAAT
                     ATAFYATNGLLRGLDLASLRRELNEQISRLSGLQHVTGVNISLFVQQIIGSLLGHAEA
                     DGESDHFRELSISNSEDAVAHGYVYVLRLYYAVLFDDFRGAANILELAEQFGPALAGS
                     PLLIVWKFADALVNLRSGNTAGLRRARASLRELKQWHQDGADHAEPKVILLQAELNWQ
                     RGATTQAIECYEVAADKARRLGLANDEALAYELAARACESNGRADFAKLFARNAHQAY
                     LRWGANAKASHLEREFPSMRDDALHPATVIGDLTDLTVRDFSSMSIRSSLDGSEINAN
                     VLDTTTVLRAAQTISSEIVLDRVLTKLLRLALEHAGAQKACMLLAHDGRLFVEAIASV
                     DGGPTRRESPATPLEASDDVPESIVHFVARTKQALVLGDATAEDVFTQDPYIVRKQPL
                     SVLCVPIVHRNEITGILYVEHRWLTGVFTAQRVEVLALLASQAAISIENARLYADLHD
                     TQEQYRALYD"
BASE COUNT         2444 a         4475 c         4091 g         2053 t
ORIGIN      
        1 caccccagac ggcgctgaac ttgtagtacc agggcagcag cgtgttggag ttgttcgcca
       61 cgtaggcgac ggaaaagtcg tcggcaagaa aagcttgggt gagcacgccg aaggccaacg
      121 cgcagaacac tgcctggccg gtgaccagcg acaggttcgc ccggagcagc ctgccccccg
      181 ccagattcag accgccgagg ccgaacacgg caagcagccc gctcaggcag agcgcgatga
      241 ccagtgccag atgtcccagc tctacgatca cggcgggccg gcgtgcatgc cgtccagctc
      301 cggaggcatg tagttctcgt cgtgcttcgc caacacctcc tcggcccgga agacgccgct
      361 cgggtcgagc tcgccgcgga cgaggattcc ctgaccttcg cggaacagat cgggcaggat
      421 gccgctgtag agcaccgtga aggctgcgcc ctggtaatcg gtgagcacga actccacgtc
      481 gagctcattg cccgtgcgcg ccacgctgcc ctcgagaacc atgccacctg cccggatctg
      541 cgtgcccagc ggcgcttcgc cggccacgat ctgatccggc gggtagaaga gattcagatt
      601 ttcgttgagc gccaccagca cgaagaacgc cgtcgccatg gtcccgagga gcaggaagga
      661 cacaacgatc aaccggttac gccgtttcgg attcacgtcc gcacaccctc cgccgcctgc
      721 cttcgtcttt ccacgtccgc ctgctcacgc aggtagcgcc ggcggccggt gatcggcgcc
      781 aacacgttga ccagcaccac ggccagcgtc agcccgtagc tcaaccacac gtacgggccg
      841 tgcccaccca tcgtcagaaa gtcaccgaac gtttcgaagc tcaccgggcc tcctcgagca
      901 ccatgtcgcg cacccactgc gtgcccgctt cccgctgcag gatctccagt cgaagacgac
      961 tcaacaggtt ggcaccgaac aggcagtaga tgccgacgac gttcaccgcc agaggcagcc
     1021 acatctccac cggcatgctt ggcgccgcgg tgagcttgaa ggtagcaggc tgatgcaggg
     1081 tcaaccacca gtcgacggag tacttgatga tggggatgtt caccaccccg atgacggcca
     1141 gcacggcgca ggcgcgcccg gcggcctcct gccggggtat ggcctgacgc agcgcgtaga
     1201 tcccgaagta caaaaagagc agcaccagca tggaagtcgt gcgcgcatcc cacacccacc
     1261 aggcgcccca agtgggcttg ccccagatcg cacccgtcat cagcgccagc accgtcatcc
     1321 acatcccgaa cggcgcaacc gttgccagca ccatgtctgc aagcttcatg cgccaaacca
     1381 gcaggaccac gccggcgcac cccatcacga gataagcggt ctgggccacg atggcggtgg
     1441 gcacgtggac gtaaatgatc cggaaactgt tgccctgctg atagtcgggc ggggcgaagg
     1501 cgagccccca gatacatccc gtgcccagca gcagcgccgc cagccctgcg aggatcttca
     1561 gccagggacc gctgatgccg tagaaccagc gcggggatcc cagccggtgc cagaattcct
     1621 tcagcgtcgc caagggccgc cctcacgtcg ccgtcgtaac gttgtcgttt gccgagcaat
     1681 catatgcatc cgcgatatgc atccgcgtag tttgacgccc aggaggggcc ggggttcgcc
     1741 tttcatcgtc actgctccac gctgatcttg agcgctgcct gaacgccgaa gggcgccgtg
     1801 gtcagcgcca gcatcgtgat ggcggcaagc cagtagagct gacctgccgc ccccgtcccc
     1861 gccatgtccg cgctgaccgc gccggcgcca aatattaaaa ccggcacata caaaggcaaa
     1921 atcagaagcc ccagaagcac cccgcccctg cgcagcccga cggtgagcgc agcgcccacg
     1981 gcgcccaaaa ggctcagggc cggcgtgccc agcagcaggg tcgccgcaag cgttggcacg
     2041 ctggcggccg gcagagacag gatcaacgcc gccagcgggg cgagcagggt catggccagg
     2101 ccgctgacgc accactgcgc ggtaatcttc gccagcaggg gcacgaagag cggctcggcc
     2161 agcaacagca tctgctccag cgtcccgtca tcgtaatcgc tgcgaaacaa cgcttccagc
     2221 gacaacatgc tggcgagcag caccagcacc cagatgatgc ccggggcgtg ctcgcgcagg
     2281 tcgtcgggcc cgcccccggc gccaagggca aagagcgtca tcgcgaggaa aaggaacacg
     2341 agcgggttca ccgccacacc caccgatcgt gccgtcgtct gcagctcgcg ccgcagctga
     2401 gcccggaaca ggccggccac cgtgttctcc ttcgcggagc cgctcatcga cccaggctca
     2461 gcaggcgcga acgctcgagc ccgaggggct gatgggtcgc tgccaccacg gccccgccgc
     2521 cgtccatatg cgcggtgacg agcgtcacgg cgagctcctg ccccgggccg tccagcgcgg
     2581 tcagcggctc gtcgagcaac cacagcggcg agcggcagac gagcagccgg gccagcgcca
     2641 cccgacgctg ctggccggcg gacaggtgct ggcaggcggt acgctcgtaa ccggccatgc
     2701 ccacacgctc cagcgccggc agcacggcgt ctgcggttcc ccgcaaagcg gcgtaccaag
     2761 ccaggttctg ctcagccgac agcagcgggt tcagacccag ccgatgtccc gcatagagaa
     2821 actcggcagt ctcgacgacg ccttcaaagt ctggatacag gccggcaatg cagcgcagaa
     2881 gcgtcgactt tccgctgccg ttggggccat gcagctccac gcactcacca gccccgagtt
     2941 ccagggagag ctccgtgaac agctgccgac cgcctcggcg gcagccggcg tcgacgaccc
     3001 ggagcaacgg cgcagctgct gctccaacct ggtctggttc caaaggcgct ggcaaggcga
     3061 tgaccgtcca gacctacccg gcggcaaccc ggtagcaggg ctcgtagggg ccgatgagct
     3121 tcatgcggcc gttctcggag aagcttcgca gcagctgctc gagcgcgccg atgaggtgcc
     3181 ggtcgctgtg cagctcgaac ggcccgtggc tttcgatcat gcgaatgccg tcgtccttca
     3241 cgttacccgt gacgatggcc gagaaggcgc gccgcagatt gaccaccagc tcatgggccg
     3301 gcaggtcggc gctcaggcgc agggcggcga cgttttcatg ggtgaccttg aacggttgct
     3361 ggtgagcgag gggaacgttg agcaaccagt tgaaatagta ggcgtcaccc acctggcgcc
     3421 ggtgccggcg cacccgacgg acccggcggc cgatccgggt gcccacctcc tccgcgtcgc
     3481 ctatcacgat gtcgtagaag cgggccacct cgtcgcccag cgaaagcctg aggaaggcat
     3541 cgagctcaaa aaagtagtcc gcgctctccg cgggaccggt gaagatcaac ggcatctcct
     3601 cgccctcgtt ggcgggatcg agcagcacgc cgagcaggta gaggatttcc tccacggtgc
     3661 ccgcgccgcc cggaaacacc agcacgccgt gaccgatgcg gaggaaggcc tctagccgtt
     3721 tctcgatgtc gggcagtatt accagatcgc tcaccatggg gttcggcggc tcggcggcaa
     3781 tgattcccgg ctccgtgagg cctacgtaac gagcgtcctg ctggcgctgc ttggcgtggc
     3841 cgaccgccgc gcccttcatc ggtcccttca tggcccccgg accgcagccc gtgcagatgt
     3901 ctaggccccg cagaccgagg tggtagccga cttccttggt gtactcgtac tcggtccggg
     3961 aaatcgagtg gccgccccag cagaccacgg tgcgcggcgg caggtcggga cgtagaaccc
     4021 gggcattctt gaggatatgg aaaatcgcat ccgtgatccc ctcggagctg ttcagatcga
     4081 aaagatcgct gtccgctatg tccgtaccga tgtagacgat gtcccgcagc accgagaaga
     4141 ggtgctgacg caccccctcg atcatccggc catcgacgaa cgcggaagcc ggcgcgttct
     4201 tgatgatcag cttgacgccc cgcgtgcgtc gggcgacctc gatgctgaag tcggcgtact
     4261 ctctgaagat ggcggcagca tcgtcggatt cgctgcccgt gttcaggacg gccagcgcgc
     4321 agcgccggaa cagatcgagc agcggcgact cggccgcggt gctggccagc gcgttgacct
     4381 cgagctgcga gagcagctcc agggatcccc tcgggctgac gtggacgtcg accttgcgaa
     4441 tctgaccggt tgtggtgttc atgccgggaa gtctaccgat tcgcgctgcc gacacccacc
     4501 gctcgatgcg ccgcgccggc cgagccggcc cgcatcgggc ggtaacccaa agcggattct
     4561 gatagtgtac ccgcggggcg cgcgtccggc ctgacgcgct tgcgggcacg gtcgattcga
     4621 aggcgatcgg cccgggaaag ccgtgtgccc cagcccaaga gaatttcgat cggaagagga
     4681 ggatcgatgt ccgggtccag catgatccgt gcactgctcg ccgtggccgc gctttgcagc
     4741 gcgaccggcg ccgccactgg caacgagccg acggacaccg ctggccccgc gctggaggag
     4801 ctcgtcgtca gcgcgacacg cacgaaccgc acggttcgcg aggtcccggc tgccgtcagc
     4861 gtggttggcc gggaggacat ccagctcggg cggcagcagc tcggcctcga cgaggccctc
     4921 ggtcgggttc cgggactttt ctcccagaac cgctacaact tcgcccagga cctgcgtctg
     4981 tccatccgcg gattcggcgc cagggcgaat ttcggcatcc gcggcctgaa gctgattgcc
     5041 gacggcatcc ctgcgaccac ggccgacggc cagagtggcg tcgacgacat cgacctgggg
     5101 tcgctcgacc gcatcgaggt gatccgcgga ccgtcgtcct cgctgtacgg cgcgtcctcc
     5161 ggtggcgtca tcaacctgat gacggaggac ggcccggagc gtccgttcgt cgaagccggc
     5221 gtcaccctcg gcgagtacga ccagcagcga taccagctca aagcgggcgg tcagaccgga
     5281 cggctgaact acctggccag cgcctcgcat ctggcttacg acgggtatcg cgagcactcg
     5341 agggtggagt cgacaatctt caactccaaa ttccggtacg ggatcgactc ggcgtcgaac
     5401 ctcacggtga tcgtcaacct ggtcgactcg ccgacggcag acgacgccgg cgccattacc
     5461 caagcccagg tcgaggacga tccccggcag gcgcagcaga ggaacttgag ctccaacgcc
     5521 ggtgaatcca tcgaacagca gaagctcggc atcatctatg accgcgctct cgacgaccgc
     5581 aacgcgatca cgctgcgcaa ctactacgtc tggcgcgatt tccaggcgtt tctgcccatc
     5641 ggcacccaca tccccttcgt cgccgacgac ggcgtggtgg agttcgaccg cttcttctgg
     5701 ggcggcggaa tccagtggcg cttcgccgat tcgctgttcg gccggccgtt ccagatgagc
     5761 gctggggtgg acgcggacat ccaggaggat gatcgccagc gctatctcaa cgaggccggc
     5821 gtaaaagggg ccctggcctt cgaccagctg gagaaggccg aagcctacgg cgcctacctg
     5881 cggggagaaa tcggactggc cgattcggtc cgccttgccc tggggatacg ctgggacacg
     5941 gtcgacctgt cggtggacga cagtttcttg gacaacgcag accagtccgc ggacctggac
     6001 tttgacgagt tgagcccctc ggtcggcctg gtctgggatc tgggcgagaa ctggagcgct
     6061 tacgcgaact acgccaccgc cttcgagaca cccaccttca ccgagcttgc cgttcccgca
     6121 agagacctcg acgtgagcct tggcggcttt gcgaacgtct cggcccagga ggcgcagagc
     6181 ttcgaggccg gtttgcgcgg cggcctgctc gacggcgccc tgtacgtcga ggcagccgct
     6241 ttcaccatgg aagtggacga cgagatcacc agcgtttcca acatcggcaa tcggtccttc
     6301 ttcgccaacg cggacaccga ccggcgcggg atcgagacct tcgccaacct cgccttcgag
     6361 ggcgggctcg agctgagcct ggcgtacacg tactcggact tcgagttcga tcggttcgag
     6421 gacaacgagg acttcgaagg caacgccctt cccggactgc ccgagcatca gctctttgct
     6481 gagatcaagt accggcatgg ctcgggcgcc tacgccgtcg ccgacgttct gcacgtcagc
     6541 aagctgtacg tgaacaacgc caattccggc accagcgaag cgtccaccgt cgccaatctt
     6601 cgagtcggcg cgtccttggc gctgggccag tggcagctcg atccgtttct cggcgtcaac
     6661 aacctcttcg acgagaacta catttccaac gtgcgcatca acgggttcgg cggcaggctg
     6721 ttcgagccag ctccgaaacg gaacatctac ggcggcgtgg ccgttcgcta cgtgctgcgc
     6781 tgatggttcg gggagcgccg attaccccgc gctctgcagc gctctgacgc tggcgtaggt
     6841 ggatacgcag taaggcacca gcacggtcag cgccatcttg ccggcctgca ccgggccgac
     6901 gtctccggac aacagcgcgt cgccgtggtt gatggcgatg agaatgaccc cgaccagaag
     6961 cgccgtccgg gcggctcgcg cgaccacgtc cgggcgggcg gccagacgca gaaacccgtg
     7021 ttgcgtgctc atcgcagacg aagggcgaac gataccgcgc cagactctcc gggctcgaga
     7081 gcctgctggt aggcccagcg aatcgaggca tattcggagg gctcggcgag ccgctgcccg
     7141 tctccgcgtt ggaccatcag gcccgacggc tcggcgaagg tttctccatc gaccgagaag
     7201 gtaatgaagg tgtcctcacc cgccgcgctg ccctccacat actcggccac ctcgggaatc
     7261 gggttggtga tgacgatgct tcctgcggcc acctcctctc gcccctcgtt ggcaaacgtg
     7321 atggtgtaga gcagctcgtc gccgggaagc acctcgtccg cggccacgag ccgtcgctcg
     7381 acctgcccgt ccaggccctg cgcgatctcg tacctggcca ccgtgttgga gagcgttacg
     7441 cgcgctgctg cttcaggggg gtatgccgtc gaggcgagga ggccggtggc aacgagcacc
     7501 aaccagaccg tgtttcgcct cagcacattc aagatcacac ttcctccaga gccttccggc
     7561 acgcttggac cctccgttcc gggaatcgat gttctcacac ccagcgcatc gcagaccatt
     7621 accgagcagg tttgatgtca atgctgcggg tgagagggtc gaagtagatg accgcatcac
     7681 cactttcgag ctgcttgcga accagcgcgc gcttctccgc gagcgtgaat tcccgatgtc
     7741 cgtagtcggt gccctcgcgc agcacgaacg actcgacgac gcccgcaagg gcttcctccg
     7801 agagctccga caggggaact ttgatcgccg ccccatcatt ttcgtgacct gatccgcatc
     7861 ccatgtcgct cgccttgaat ttctgaacct aaggtgcgtt acttcccctt actgaaatcg
     7921 tcataccggc gttccaacag gacggggcac catggccgaa ccagatcaca tactcgtcgt
     7981 cgaagacgag ccgattaccc gcgagcaact cgtatcctac ttcgaggaag aaggtttccg
     8041 cgtcacttcg accggatccg gcgacgaggt gcacgagctg gtaaagaaca gcgacattat
     8101 actcgttctc ctggacatca agctgcccgg caaggacggt ctgacgttga cccgcgagat
     8161 ccgcgctcaa tccgacgtcg gcatcatcct ggtaacaagc aaacaggaac agatcgaccg
     8221 cattctcggt ctggagagcg gcgccgacga ctacgtgacg aagcccttcg acccgaggga
     8281 gctgctcagc cgcgcccgca acctgattcg gcgcgtccag cttcagcagc gccagcgccg
     8341 caagaaccac atccgcaact tcgagggctg gcagctagat ctgaacaaac gcgagctgac
     8401 ttctccggaa ggcgagaaga cgacgctgtc ggcaggcgag taccagctcc tgctggcatt
     8461 catggagcag gccggcgagg tcatgaaccg cgatcagctg atgaaccgca tccgtaaccg
     8521 cgagtggtat cctgacgacc gatacatcga cgtgctggtc ggccagctcc gcaaaaagct
     8581 cggcgagcgg gcggccaacg ccaagatcat cgccaccatc cacgggacgg gatacctgtt
     8641 cacacccgaa gtggcctgaa ttcgttcagc aaacgggatg tgacgatcct gaacagcgtg
     8701 tgggggcaca gacgggggaa aaggcgtgag cagcgtggat gaaaagatct acgaaagcgc
     8761 gtcgacagtc gtccgacgga ccacctggca ggatcagccc gtcgttgcga aacggctgaa
     8821 gcccgagggc ctgccctcga acgtgctcag ccagtttcat cacgaattcc acctgaatca
     8881 gtcgctgacc agccccaacg tctgtcgcgc gctcgccatg gacgacagcg gcctcgagat
     8941 ctacttcgag gacacccagg gcgcctctct gcgcagcctc atcgacaccg gcgagctttc
     9001 cctggacgaa cgcatcgcga tcgccgtgga gctcagccgc gcgctgcagt ccgttcacga
     9061 cgagggcgtg atccacaggg acgtcaaccc cggcaacgtg ctcgtctccg aagaccctat
     9121 gcgcgtgcac ctcatcgact tcggcttggc tacgctggca gcccgggaat atcccgaggt
     9181 ggaacccctt ggccaggtga ccgggaccct gccctacctt tcacccgagc agaccggccg
     9241 cgtgaaccga gtggtggact accgcacgga cctgtactcg ctgggcgcga cgctctacga
     9301 gcttctggcc ggcaaccccc cgttcaccaa cacggacccg ctggagctca tccacgcgca
     9361 catcgcgagc acgccgcggc ccctaaccgc catcggcgag ggaattccgc gctggctgtc
     9421 ggaaatcgtc cagaaactgc tcgccaagca accggaagac cgctaccaga gcgccgcagc
     9481 cgtccgcgac gatctgatca gggggcagac ccaggccaac gtggtaccgt tccggctcgg
     9541 acagaccgac tcgcccgggc tgctggccat gcccaaacgc ctgtacggtc gcaccgccca
     9601 gatcggcatc attcaggaat atctggagcg cgccgcacga ggggaagttc tcttcctgca
     9661 gatcaccggg gcgcaaggca gaggaaagtc ctccctatgc gcggagctcg ccaggcaggc
     9721 agccggcaca aaagccatgg tcgcccggac caatgcgctt gccttcgact ttcgcgacac
     9781 cgacgcggtc tggatcgagc tgacccgcca gctcctgcga caggcgctgg ccgacgctaa
     9841 agccggtgac cttctggcgc gcatcgcggc gctgaactcg ccgaacgttc gcagcctgac
     9901 ggagctcatc ccggagctga gagatctggt gcctgcgggc agtgagggcg cggggctgcc
     9961 cggggtgggc atccaggaag tcctgaaagc gctgcagccc caaccgcttt gcctgatcgt
    10021 ggaggaagtc gacaacgtcc cttcggaaag cctggaaacc ctgctgagca tggcgctcga
    10081 ctcgcggcac ttgctgatga cggttacccg ggcgaacgcc gattcggacg cgttcaggac
    10141 gccccgactg gcgaccaagg cggccacgct ggagctgccg gcatttgcgc gggccgacgt
    10201 gagaagcctt ctgtcggaca tgctctcgac cagcgaggtt cgagttcgcg agctcgcaag
    10261 cgagctgcac gccaagaccg acggcctgcc cgccgacgtc cttcagctga tcgaggagct
    10321 gcatcacctc ggccacatct tccgtgacgc caatgcggcc gagtgggagt gggacatggc
    10381 ggccatccgc gcccactact tcagcaacaa ctccgcagac cgaatacgcc ggctgatcag
    10441 cgagctttcc gaggagacga cgcacgcgct gcaggtcggt gcggccgtgg gagagcaatt
    10501 ctcgagcgag ctggccgcca ccttgctcga cgtcgagcag gccgagctcg ccgggcggct
    10561 gcggccggcg ctgaccaaag gcctgctcac caccgcgacg ggcagcaagg aagcgccggg
    10621 agatcagcac cagctgctct accggttcgc ccatccaagg gtgaggtcgc tggtgtacgc
    10681 ggagatggcc caggaagcca agtcgacgat tcacttcgcc atcgccgatg agtgcaagcg
    10741 cggccacagg ccggcgggca ggacgctcct gcagatcgcc catcacctca acgccgccgc
    10801 ggatcccatt gggctcgaca aggacaaacg cctggaaatc gcgcactaca accttctcgc
    10861 cgcaaaggag agtatcggcc agggcgcctt ccagcccgcc tacaagttct gccgcagcgg
    10921 gctgtcgctc ttgagcgagc tgcccgacga gcccatcttc atcgagctgt gccagagcgc
    10981 tgccgaggcc gccttctact gcagcgactt cgagcagctc gaccgggtca tcgccagcat
    11041 tccgcacaat accagcgccc tggacgagat cagggttcgc gcggcggtgg tccagaatca
    11101 cctgggcgca gcccgcgagc aggcgttcga cgccctcgag agcctggggt acgtgcgacc
    11161 cgcaaccggc atcaacccca tcggccaggc tttccgcagg ctcggcagga agcgcgcgcc
    11221 ggacgccgcg cccaaaccgg ccccggtgct cgaggacatt cgcctgcagc acaccttccg
    11281 gttgctcggc tacctgttgc acgccggata ccacctgggc agcgagggcc tggacgccct
    11341 tacagacgac atgctcagcg aggcggcacg gcacggctac agcgccgaga tcgcctttgc
    11401 gttcgccgcg cgtgccgtca gcgccgtcgc cgaccaccgg atcgggatcg cgcagcgcta
    11461 cgccggggag gcgcgagccc ttgccggcca gttctcaggg gacgccttct ccatccgcgc
    11521 ggaaaccctg ctgaacggcc tggtggaccc atggtcgggg gggctggacg acacgcttgg
    11581 cgggctttcg cgcaacatcg acgccagcat ggcctgccag gactacgagt ttgctgccac
    11641 ggccacggcc ttctacgcaa ccaacggcct gcttcgcggg ctcgatctgg cgtccctacg
    11701 gcgagagctg aacgagcaga tttccagact ttccggtctt cagcacgtca cgggcgtcaa
    11761 catctcgctg ttcgttcagc agatcatcgg cagcctgctg ggtcatgcgg aagccgacgg
    11821 cgagtcggac cacttcaggg agctgagcat ttccaacagc gaggacgcgg tcgcccacgg
    11881 ctacgtgtac gtgctgcgcc tctactacgc cgtgctcttc gacgatttca ggggggcggc
    11941 caacattctc gagctcgccg agcagttcgg ccccgcgctg gcggggtcgc ctctgctcat
    12001 cgtctggaaa ttcgccgacg ctctggtgaa cctgcgctcg ggcaacacgg ctggcctacg
    12061 aagggcacgc gcgtcgctgc gtgagctgaa acagtggcac caggacggcg ccgaccacgc
    12121 ggagccgaag gtcatcctgc tgcaggccga gctgaactgg cagcgcggcg ccaccaccca
    12181 ggccatcgag tgctacgagg tcgccgccga caaagcgcgt cggctcggcc ttgccaacga
    12241 cgaggcgctg gcctacgagc tggcggccag agcctgcgag tccaacggtc gggccgactt
    12301 cgccaagctc ttcgcgcgca acgctcatca ggcctacctg cgctggggcg caaatgcgaa
    12361 agccagtcac ctcgagcggg agttcccgag catgcgcgac gatgcgctgc atcctgcgac
    12421 cgtgatcgga gacctgaccg acctcaccgt gcgcgacttc agctccatgt ccatccggtc
    12481 gagcctggat ggaagcgaga tcaacgcgaa cgtcctggac accaccacgg tgctgcgcgc
    12541 ggctcaaacc atctccagcg agatcgtcct cgaccgcgtc ctgacgaagc tgctgcgcct
    12601 ggcgctggag catgccggcg cccagaaagc ctgcatgctg ctcgcccacg acggaaggct
    12661 gttcgtcgaa gccatcgcct cggtggacgg cggcccgacc cgccgcgaga gcccggcgac
    12721 cccgctggag gcctccgacg acgtgcccga gagcatcgtt cacttcgtgg cgcgcaccaa
    12781 gcaggcgctg gtgcttggcg acgccaccgc ggaggacgtc tttacccagg atccatacat
    12841 cgttcgcaaa cagccgctgt ccgtcctctg cgtgcccatc gtgcaccgca acgagatcac
    12901 cggaatcctc tacgtcgagc accgctggct caccggcgtt ttcactgccc agcgggtgga
    12961 agtcctcgcc ctgctcgcct ctcaggcggc gatctcgatc gagaacgcgc ggctgtacgc
    13021 cgacctgcac gacactcagg agcagtaccg agcgctgtac gac
//
LOCUS       DBNHKV010000214         2416 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1731889, whole genome shotgun sequence.
ACCESSION   DBNHKV010000214 DBNHKV010000000
VERSION     DBNHKV010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1731889"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1429
                     /locus_tag="AC16PP_06860"
     CDS             <1..1429
                     /locus_tag="AC16PP_06860"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6481384.1"
                     /translation="EYSEGIAAGFKARLTHVNVNYRYVDHELIYLLDNSDAAVVVYAA
                     EFAHHVAAIRDQLPGVGQWIEVPDGHPGLDDALDYERLVADGDGAPLDLDRSPDDMLF
                     LYTGGTTGMPKGVMWRHQDLWCVTGAGGNPRIGVEPSPDLQSYVSRLQAEPPPVNLPL
                     PPIMHGTGLLSVVGAMAHGGTAVTLTGRSFEAEAALEAIQRHAVTSTTIVGDAFARPM
                     VEALDAAPGRHDISSLKIVSSSGVMWTRAIKAGLLRHNPDLVLVDAFSSSEAIGLGTS
                     VMTRDEEIEVAKFTLGPSCKVFGEDLREIAPGSGETGMVGVTGFLPVGYYKDEEKTAR
                     TFPVVDGVRYSIPGDWVRVEADGSLTLLGRGSNCINTAGEKVYPEEVEEALKHHESVA
                     DALVVGLPDERWGQAITAVITLHPGYSLDEIGLREFARRELAGYKLPKRILAKDDLER
                     APNGKANYAAIREFAQAELGISSGS"
     gene            1565..1921
                     /locus_tag="AC16PP_06865"
     CDS             1565..1921
                     /locus_tag="AC16PP_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481385.1"
                     /translation="MSERDEQEKLEEQACALLLRSPHLSVSQVLDLMDIGDSEFREML
                     RSNPRIASLMEQRREGALPRVESKMTECPACGEWFIPYASARCCSDTCAKIDQISNRP
                     NAARRFRLRNRQTGGS"
     gene            complement(1946..2227)
                     /locus_tag="AC16PP_06870"
     CDS             complement(1946..2227)
                     /locus_tag="AC16PP_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481386.1"
                     /translation="MISLTFYGLACLCGIAAVLILLVGFVDWLQTERWTNLSVLEFGY
                     DAHLIRARWFINNRWSWWLHDLMHLVPLYVFLLALAPLSWVVGAWFGRR"
BASE COUNT          459 a          789 c          776 g          392 t
ORIGIN      
        1 cgagtacagc gagggcatcg ccgccggctt caaggcccgg ctcacccacg tgaacgtcaa
       61 ctatcgctac gtcgaccacg agctgattta cctgctcgac aactccgatg ctgcagtggt
      121 cgtctacgcc gccgagttcg cccaccacgt tgccgccatt cgcgaccagc tgccgggggt
      181 cgggcagtgg atcgaagtac ccgacggcca ccccggcctc gacgacgctc tcgactacga
      241 gcgcctcgtc gccgacggcg acggcgcgcc cctggatctc gaccgctccc cggacgacat
      301 gctgtttctc tacaccggcg gtaccacggg catgcccaag ggcgtcatgt ggcgtcacca
      361 ggatctgtgg tgcgtcaccg gcgccggcgg caacccgcgg atcggcgtgg agccgtcccc
      421 cgatcttcag agttacgtgt cccgtctgca ggccgagccg ccgcccgtca acctgccgct
      481 gcctcccatc atgcacggta cggggctgct atccgtggtg ggcgccatgg ctcacggcgg
      541 aacggcggtg accctgacgg gccggagctt tgaagcggaa gccgcgctgg aagcaatcca
      601 gcggcacgcg gtgacgtcca cgaccatcgt cggcgacgcc ttcgcccggc ccatggtgga
      661 agcgctggac gccgcacccg gccgccacga catctcgtcg ctcaagatcg tgagctcctc
      721 gggcgtcatg tggacgcggg ccatcaaggc cggcctgctg cgtcacaatc cggacctcgt
      781 tctcgtcgac gcgttttcct cctcggaggc catcggcctt ggtacctcgg tcatgacccg
      841 cgacgaggag atagaggtcg ccaagttcac gctggggccg agctgcaagg ttttcggaga
      901 ggacctgcgg gagatcgcgc ctggatccgg agagaccggc atggtgggcg tcaccggctt
      961 ccttcccgtg ggctactaca aggacgagga aaagaccgcg cgcacctttc cggtcgtcga
     1021 cggcgtgcgc tactcgattc ccggtgactg ggtccgggtc gaggcggatg ggtcgctcac
     1081 gctgctggga cgcggcagca actgcatcaa caccgccggg gagaaggtat atccggagga
     1141 agtcgaggag gcgctgaagc atcacgagtc ggtggcggat gccctggtgg tgggactccc
     1201 cgacgagcgc tggggccagg cgatcaccgc cgtgatcacg ctccatccgg gctactccct
     1261 ggacgagatc ggtctgcgcg agttcgcccg tcgggaactc gccggttaca agttgcccaa
     1321 gcgcatactg gccaaggacg acctcgaacg cgcacccaac ggcaaggcaa actatgcggc
     1381 catccgggaa tttgctcagg cggaactggg catttcatcg ggttcgtaat accgtctgga
     1441 ggacgggccg taggcgatcg tcatttaccc agggcaacat tgcgcggcga catgagcgca
     1501 cgactggccg tgacccggcc ggccgacacc gattgagtca tctgccggca ggagtattgg
     1561 ccgcgtgagc gaaagggatg agcaggagaa actcgaggag caggcgtgcg ccctcctgct
     1621 gcggtctccg catctcagcg tcagccaggt tctcgacctg atggacatcg gcgactccga
     1681 atttcgcgaa atgctcagga gcaatccgcg catcgcctcg ctcatggagc agcgtcggga
     1741 gggcgctctg ccgagggtgg agtccaagat gacggagtgc cccgcctgcg gagagtggtt
     1801 cattccctac gcgtctgcgc gctgctgctc ggatacctgc gccaagatcg accagatcag
     1861 caacaggccc aacgcggcgc gacgctttcg tctgcgcaac cggcagaccg gcggctcctg
     1921 acgtcgacca cgcccagtcg ggggttcagc gccgaccgaa ccaggctccc accacccagg
     1981 acaggggggc cagagcaaga agaaacacat agagcgggac caggtgcatc aggtcgtgga
     2041 gccaccagct ccagcggttg ttgatgaacc agcgcgccct gatgagatga gcgtcgtagc
     2101 cgaactcaag gacgcttaga ttggtccagc gctctgtctg cagccagtcg acgaaaccga
     2161 ccagaagaat cagcaccgcc gcgatcccgc agagacaagc gagcccgtag aaagtcaagg
     2221 aaatcatgcg atcagccatg gtgccagtcg atccgtctga agatgaacca ttgacccaag
     2281 cgtagagttc cggatgcgcg aaggcgatgc cgtgacggct ttcacgaatc ccgggaagcc
     2341 gaacccgttc cagaacggct cgaggacggc ggtttcacaa tgcgcgcaag gtctttaccc
     2401 ttctcctttc atccac
//
LOCUS       DBNHKV010000215         3909 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_102768, whole genome shotgun sequence.
ACCESSION   DBNHKV010000215 DBNHKV010000000
VERSION     DBNHKV010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_102768"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="AC16PP_06875"
     CDS             complement(<1..609)
                     /locus_tag="AC16PP_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6481387.1"
                     /translation="MSETHIVPVPQSVLDVCYINDDQYEAMYERSITDPEGFWSEQAD
                     KFVAWYEKPHQVREADMVRGNISWFAGGKLNIAYNCIDRHLPDRASQTAIIWEGDDPA
                     NDKTITYQELHDAVCQLANGLRSRGVGKGDRVCIYMPMVPEAAYAMLACMRIGAIHSV
                     VFGGFSPQSLKDRILDSDCRTVITADHGVRGGRLVPLKENTEA"
     gene            complement(646..2208)
                     /gene="pgi"
                     /locus_tag="AC16PP_06880"
     CDS             complement(646..2208)
                     /gene="pgi"
                     /locus_tag="AC16PP_06880"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR6481388.1"
                     /translation="MRPDDLAPWKSLTRLSADSIKQPLSDWLRSPERFARLSFEVGEL
                     LVDLSKQRLNEEIVDALLALAGAARLDRRIDDLFEGAQVNPTEQRPALHTALRAPLAS
                     RPAAYGEAVETTLTRMLDFAERVRSGVWRGATGKAIKSVVHIGIGGSHLGPELAVRAL
                     EGAKGAGPSVRFVANIDRSALDTALTGLDPETTLFIVASKSFTTLETRVNADSARAWF
                     LERMADLDALKHHFVAITENVEAARAFGMGPEQIFPMWDWVGGRYSLWSAVGLPVAIS
                     AGADAFRELLAGAHAMDEHFRSSPLSGNVPVLMALAGVWNYNFLGVSNHAILPYSARL
                     GLLPSYLQQLEMESNGKSVDLEGEAVTVHTAPIIWGGEGTNGQHAFHQLLHQGTRSFT
                     ADFIMVGQPDPDDVHGRWLLANGLAQSQAMMAGQDADDAHRRVAGDHATTTIVMRQLN
                     PFSLGSLLALYEHKVFCQGVIWNINSFDQFGVELGKTLSVPIFQQLGGNSAMTQDAST
                     RGLVELLRGTTG"
     gene            complement(2333..3085)
                     /locus_tag="AC16PP_06885"
     CDS             complement(2333..3085)
                     /locus_tag="AC16PP_06885"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR6481389.1"
                     /translation="MSIIKASDQLIRSMTPPAQHSLAIETTAALGQKLEGKSLPRYIV
                     IEGPIGVGKTTLANKLAEAFHYPILLEPAAQNPFLNSFYRDGRRNALPTQLFFLLHRA
                     QQIANLDHADLLGPTLVADFLMEKDDLFARMTLDEDEYALYRQIHETLDIDPPKPDLV
                     IYLQAPVDVLRTRIRDRGIDYERDIEAGYLESLARAYTEFFHFYDAAPLLVVNAASID
                     FANNAVHFESLLDQILAMDGLRQYFNPNPTLL"
     gene            complement(3100..>3909)
                     /locus_tag="AC16PP_06890"
     CDS             complement(3100..>3909)
                     /locus_tag="AC16PP_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024040.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA nucleotidyltransferase/poly(A)
                     polymerase-related protein"
                     /protein_id="HIR6481390.1"
                     /translation="GGLEDLERADLRFIGEAEVRLREDPVRLLRAVRFQAKLNFQLDE
                     SIETALPDAAERLSVIPSARLFDEVAKLMMSGAAGGAWKAIAGTPLRAALFPGTPPDS
                     RLVELAMANTDQRIASDLPVTPGFLLAVLMWHDFQARCNALTDERKPAEARHQAASET
                     LAAQQAVTSMPRRFSQFVRDVWSLQPRLEARLPRQIDRIVQHPRFRAAYDFLVLRAES
                     GEQATDSEPLDDAAQWWTTFQQADQATRADMTEARLAVQPPRKRRRRRRRG"
BASE COUNT          638 a         1217 c         1332 g          722 t
ORIGIN      
        1 cgcctcggtg ttctccttca gcggcaccag ccggccgccc cggacgccat ggtctgccgt
       61 gatcacggtc cggcagtcgg agtcgaggat ccggtctttc agagactggg gcgagaaacc
      121 gccgaacacg acggagtgaa tggcgccgat gcgcatgcag gcgagcatgg cgtaggctgc
      181 ctccggcacc atcggcatgt agatgcagac tcgatcgcct ttgcccaccc cgcgcgaccg
      241 aagcccgttt gcgagctggc agaccgcatc gtgcagttcc tgataggtga tcgttttgtc
      301 gttggcgggg tcgtcccctt cccagatgat cgccgtctga ctcgcccggt cgggcaggtg
      361 tcggtcgatg cagttgtacg cgatgttgag cttgccgccc gcgaaccagc tgatgttgcc
      421 gcgaaccatg tccgcctcgc gtacctgatg cggcttctcg taccaggcga cgaacttgtc
      481 tgcctgctct gaccagaaac cctcgggatc ggtgatcgac cgctcgtaca tcgcttcgta
      541 ctggtcgtcg tttatgtagc agacgtcgag cacggactgc ggtacgggaa cgatgtgcgt
      601 ttcagacatg gcttcctccc ttggcggggt tgttcggtgc gggggtcatc ccgtcgtccc
      661 gcgcagcagc tcgaccagac cgcgcgtgga ggcatcctgg gtcatcgcgg agttacctcc
      721 gagctgctgg aagatgggca ccgacagcgt cttgccgagc tccacgccga actggtcgaa
      781 cgagtttatg ttccagatca cgccctggca aaaaaccttg tgctcgtaca gggctaacaa
      841 ggatcctaag ctaaaagggt tcagctggcg catgacgatg gtggtggtgg catggtcgcc
      901 cgctacgcgc cggtgagcgt cgtccgcgtc ctggcccgcc atcatcgcct gggattgggc
      961 caggccgttc gccagcagcc atctgccgtg gacgtcgtcg gggtccggct gcccgaccat
     1021 gatgaaatcg gccgtgaacg agcgggtgcc ctggtgcagg agctgatgga aagcgtgctg
     1081 gccgttggtg ccctcgccgc cccagatgat gggagccgtg tgcaccgtca ccgcttctcc
     1141 ctccagatcc acgctcttgc cgttgctctc catttccagc tgttgcaggt agctgggtag
     1201 cagaccaagc ctcgcgctgt acggaagaat ggcgtggttg ctcactccga ggaagttgta
     1261 gttccagaca cctgccaggg ccatcaggac cggcacgttg ccggacaagg gcgagctacg
     1321 gaagtgttcg tccatcgcgt gcgcgccggc cagcagctca cgaaacgcgt ccgcccccgc
     1381 cgagatggct acgggcaggc cgacggcgga ccagagcgag tagcggccgc ccacccagtc
     1441 ccacatggga aagatctgct ccgggcccat gccgaacgcc cgggcggctt cgacgttctc
     1501 ggtgatcgcc acgaagtgat gcttcaaggc gtctaggtcg gccatgcgct cgaggaacca
     1561 tgcccgggca gaatcggcgt tgacgcgggt ttccagcgtg gtgaacgatt tcgacgcgac
     1621 gatgaaaagg gtcgtctcgg gatcgagccc ggtcagcgcc gtgtcgaggg cggatctgtc
     1681 gatgtttgca acgaaacgca cactgggacc cgcgcccttc gctccttcca gggcccgaac
     1741 ggctagctcc gggcccaggt gcgagccgcc gatgccgatg tgcacgacgc tcttgatggc
     1801 cttgccggtg gcaccccgcc agacgccgga gcggacccgc tccgcgaagt cgagcatgcg
     1861 ggtcagggtc gtctcgacgg cctccccata ggcggcgggc cggctcgcca gcggtgcgcg
     1921 cagggcggtg tgcaaagccg gccgctgctc tgtgggattg acctgtgcgc cttcgaacag
     1981 gtcgtctatc cggcgatcca gccgggcggc cccggccagc gccagcaggg catcgacgat
     2041 ctcttcgttg agccgctgtt tggacaagtc gacgagcagt tctccgacct cgaagctcag
     2101 ccgggcgaat ctctccggcg accgcaacca gtcgctcaac ggttgtttga tgctgtctgc
     2161 cgaaagccgg gtcaggctct tccacggcgc gaggtcgtcg ggcctcatgg gctggccccc
     2221 gaagccctgg tgccgcccgg caagatcggc ctgctcgcgg caggcgggcc ggggatgctg
     2281 cgggcctgtg cccctttagc gctggcggcg gcggtgaatc ccaacggtgc gttcaaagca
     2341 gggtgggatt cgggttgaag tactgacgaa ggccgtccat cgcgagtatc tggtcgagca
     2401 gggactcgaa gtgaacggcg ttgttggcga agtctatgga cgctgcgttg acgaccagca
     2461 ggggcgcggc gtcatagaaa tgaaagaact ccgtataggc ccgggccagg ctttccaggt
     2521 acccggcctc gatgtcgcgt tcgtagtcga tgccgcggtc acgaatgcgc gttcgcagaa
     2581 cgtccaccgg ggcctgcagg tagatgacga ggtcgggctt tggcgggtcg atgtccagcg
     2641 tctcgtggat ctggcggtag agggcgtact cgtcctcatc cagggtcatc cgggcgaaca
     2701 ggtcgtcctt ctccatgagg aaatcggcga ccagggtggg cccgagcagg tcagcgtggt
     2761 cgaggttggc aatctgctgc gccctgtgga gcaggaagaa cagctgggtg ggcaacgcgt
     2821 tgcgccggcc gtctcggtag aagctgttga gaaacgggtt ctgcgcggcg ggctccagga
     2881 gaatcgggta gtgaaaggcc tcggccagct tgttggcaag ggtggtcttg ccgaccccga
     2941 tggggccctc gatgacgatg taacgcggta gagattttcc ttccagtttc tgcccgagcg
     3001 cggccgtagt ttcgatggct agagagtgct gggccggtgg cgtcatggag cggatcagct
     3061 ggtcggatgc cttaataatg gacaaggcat cccttcgctt caaccacggc ggcgccttct
     3121 cctccgcttt cttggtggct ggaccgccag tcgcgcctcg gtcatatcgg cccgcgtggc
     3181 ctggtctgcc tgctggaagg tagtccacca ctgcgcagca tcatcgagcg gttcgctgtc
     3241 ggtggcctgc tcgcccgact ccgcccgcag cacgagaaag tcgtaggcgg cccggaatct
     3301 ggggtgctgc acgatccggt cgatctgcct cggtagcctg gcctccagac gtggctgcag
     3361 gctccagacg tcgcgtacga actgagagaa ccggcgcggc atggacgtaa ccgcctgttg
     3421 agcggccagg gtctcggacg ccgcctggtg ccgggcctcg gcgggctttc gctcgtcggt
     3481 cagagcgttg catcgggcct ggaagtcgtg ccacatgagc accgcgagca gaaagcccgg
     3541 ggtgacgggc agatcgctgg cgatacgctg gtcggtattg gccatggcca gctcgaccag
     3601 ccgggaatcg ggaggtgtgc cggggaaaag cgcggcccgc agcggcgtgc ccgcaatggc
     3661 tttccaggcg cctcccgcag ctccggacat catcagcttg gcgacctcgt cgaacagccg
     3721 tgcggagggt atgacgctga gccgttcggc ggcatcgggc agggcggtct cgatgctctc
     3781 atcgagctgg aaattgagtt tcgcttggaa cctcaccgcg cgcagcagcc gcacggggtc
     3841 ctcgcgcagc cggacctcgg cctcgccgat gaagcggagg tccgcccgct ccagatcctc
     3901 cagaccacc
//
LOCUS       DBNHKV010000216         3907 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1375641, whole genome shotgun sequence.
ACCESSION   DBNHKV010000216 DBNHKV010000000
VERSION     DBNHKV010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1375641"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="AC16PP_06895"
     CDS             complement(<1..938)
                     /locus_tag="AC16PP_06895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6481391.1"
                     /translation="MVDAYLEGLYRRGRGPLLASLAGYTRRLAALTNQRAYDLVWLEK
                     ELLPWLPAAFERMLRRPYLVDYDDAIFHRYDQHPRALVRWLLGNKIDLVMRRAQVVVV
                     GNRYLADAARRAGARELEIVPTSVDTQRYRPLAKDSGRNASGDFNVGWIGTPQTAPFL
                     KTLTPVFERLHGSGGFRFTFIGVPAGLDVGVPYEAFPWAESDEVSLLQRLDCGIMPLP
                     DLPFERGKSGYKLIQYMACGLPVVASPVGENQHIVREGVNGFLASQTDEWIAALQGLR
                     DAADRGRALGMAGREIVEATYDCRRNAKRIAALLRAA"
     gene            complement(1055..1945)
                     /locus_tag="AC16PP_06900"
     CDS             complement(1055..1945)
                     /locus_tag="AC16PP_06900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6481392.1"
                     /translation="MVIVNWNSGDMLATCLDGLAAHCANVIGQVIIVDNASTDSSLDF
                     PMPTSLEVDLIRSGCNLGFAAACNLGASRSGADFLLFANPDLVIGQDILTQPLRRLRG
                     DATIGAVGIGLYDAGSGQRQRTCCRLPTPISMLARSLGIDRLWPRLGYRLSGWDHSSS
                     RKVDHVIGACYLTPRAVFDRLGGFDERFFLYLEDLDFSARIQQAGMDILYLANPCASH
                     VGGGSSRQIKDERLFYMLRSRLQYVHKHMGVWGLIIVGFSTLAIEPWLRILASVLAGS
                     VTGCGEVLRGYGRLFRWLGS"
     gene            2145..3539
                     /locus_tag="AC16PP_06905"
     CDS             2145..3539
                     /locus_tag="AC16PP_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6481393.1"
                     /translation="MPAASAGPLRFNEKLGYAFGDTASNFFFQTFNIFLLYYYTDVFG
                     ISAAAVGTLFFVSKLWDAVNDPLMGALADRTNTRHGKYRPYLLWMALPYGLMGYLMFA
                     NPDFDDTGKLVYAWVTYSLMMMLYTAINVPYSALMGVMTPSTTERTLLASYRFVGAFG
                     GGLLISMLVRPMVHYFGAEDEAAGFQVTMALFAVISVVMFWYTFGATRERVRSLPETD
                     VSFGNDVKLLLSNRPWIVLFFAGIFTLSSIAIKNAVTIHYFKYYVGDDSSPILLFFDI
                     TTLFMTIGMLALIAGVACTKWLTARFEKKTLLIALSLGNAVTMVAFFFVPPDQLALML
                     ALNITGSLMAGPTAPLVWAMYADVADYGEWRFERRTTGLVFSASMFAQKFGLTIGAGL
                     AGWMLALFGYVANVTQSDDSLMGIRLMFTLFPAAVSLLNVAVIAFYTLSDAEVESIGR
                     ELEDRKAQAGPAPI"
     gene            3604..>3907
                     /locus_tag="AC16PP_06910"
     CDS             3604..>3907
                     /locus_tag="AC16PP_06910"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIR6481394.1"
                     /translation="MAEVSEQMPQLFISYSRDDGRAVYPDADWLGGLGYAVHIDRHIS
                     PGEQWREAIANAIADSDLLIFYASPSSIESKFCSQELQFALSRDIPVITVMLEETPF"
BASE COUNT          779 a         1280 c         1112 g          736 t
ORIGIN      
        1 gcagcacgca acaaggccgc tatccgcttt gcgttgcgtc ggcagtcgta tgtcgcctcc
       61 acaatttccc tgcctgccat cccgagggcg cgaccccgat cagccgcatc gcgcagaccc
      121 tgcaaagccg cgatccactc gtcggtttgc gaggcaagaa agccgttcac accttcgcgg
      181 acgatatgct ggttttcccc taccggactt gcaaccaccg ggagcccaca ggccatgtac
      241 tggatcagtt tgtaacccga cttgccgcgc tcgaagggca ggtccggaag cggcatgatt
      301 ccgcaatcaa gccgttgcag caggctgact tcgtcactct ccgcccaggg gaacgcttcg
      361 tagggcaccc cgacgtcaag accggctggc acgccgataa acgtaaaccg gaacccgccc
      421 gatccgtgca gccgttcgaa cacgggggtc aatgtcttca gaaacggcgc cgtctgcggc
      481 gtcccgatcc aacccacgtt gaagtcaccc gatgcgtttc ttccagaatc cttcgccagc
      541 ggccggtatc gctgcgtatc caccgaggtc gggacgatct cgagttcacg ggcaccggca
      601 cggcgcgcgg cgtcggcaag gtaacgattg ccaaccacca cgacttgcgc gcgacgcatc
      661 acgagatcga tcttgtttcc gagcagccac cgtaccagcg cccgagggtg ctgatcgtag
      721 cggtgaaaaa tcgcgtcgtc gtagtcgacc aggtacggcc gccgtaacat ccgttcgaac
      781 gctgcgggga gccagggcag cagttccttc tccagccata ccaggtcgta ggcacgctgg
      841 ttggtcaacg cagcgagccg gcgcgtgtag ccggcgaggg aagccagaag cggcccccgc
      901 ccgcggcgat acagcccctc gagataggcg tcgaccaaga gaggcgaaac atctatgtca
      961 atgccgtcct cacgcagaag cggaacgtat tgcagcgttc gcagccgggt gctggctcca
     1021 agcgcgccat atctcgtcaa ggcgagaacg cggatcacga gcccagccaa cgaaatagtc
     1081 gcccgtagcc acgcaggacc tcgccacaac ccgttaccga accagccagc acgctggcca
     1141 gaatccgcaa ccagggctcg atcgcgagcg tgctgaaccc aacgatgatc aggccccaca
     1201 cgcccatatg cttgtgcaca tactgcaacc gcgacctcaa catgtagaaa agcctctcgt
     1261 ccttgatctg tcgggatgaa ccacctccga cgtggctggc gcagggattg gctagataga
     1321 gaatgtccat cccggcctgc tgaattcgcg ccgaaaagtc cagatcctcg aggtagagga
     1381 aaaatcgctc gtcgaatccg ccgaggcgat caaatacagc gcgtggcgtc aggtagcagg
     1441 ccccgatcac gtggtcgact ttgcgcgagc tggaatgatc ccatccactc agacgatagc
     1501 ccagtcgcgg ccagagacga tctatgccaa gagaacgcgc gagcatgctg attggcgtcg
     1561 gtaaccggca gcacgttcgc tggcgctggc ctgaaccagc gtcatacaaa ccaatgccca
     1621 ccgcgccaat ggtggcgtcc ccacggagcc ggcgcagcgg ttgggtcaat atgtcctggc
     1681 cgatgaccag atccggattc gcgaacagca ggaaatccgc cccagaccgc gacgctccga
     1741 gattgcaggc ggctgcgaat ccaaggttgc acccggagcg gatcagatcg acctccaatg
     1801 aggtcggcat cggaaagtcc agagaagaat ccgtggatgc attgtccacg atgatgacct
     1861 gaccaattac gttcgcgcag tgggctgcca ggccatcgag gcaggtggcg agcatgtcac
     1921 cggaattcca gttgacgatg accacgtcaa cgctccgatc tgttgcaaag caggtatcca
     1981 acgacaggcg caaccccaca agactcagct cattctagcg acacgcactc ccgggcgcgc
     2041 accctctccc gaccttttcc tcagcaatcg ccatggataa atgatagcgt tgtcatcctg
     2101 atttgcgacc cgctcgtcgc caggcgaccg ggagagggcc agacatgcca gccgccagcg
     2161 ccggaccgct tcgcttcaac gaaaagctcg gctacgcgtt cggcgacacg gcctccaatt
     2221 tcttcttcca gaccttcaac atctttctgc tctactacta cacggacgtc ttcggcatct
     2281 cggcggcggc ggtgggaacg ctgtttttcg tctcgaagct ctgggatgcc gtgaacgatc
     2341 cactcatggg tgcgctggca gaccggacca atacccggca cggaaaatat cgcccgtacc
     2401 tgctctggat ggctctcccc tacggcctca tgggctatct gatgttcgcg aacccggact
     2461 tcgacgacac cggcaagctg gtctacgcct gggtgaccta ttccctgatg atgatgttgt
     2521 acacggccat caacgtcccc tactccgcgc tcatgggcgt gatgaccccc agcaccaccg
     2581 agcgcacgct gctggccagc tatcggttcg tgggtgcgtt cggtggcggg ctgctgattt
     2641 cgatgctcgt ccgacccatg gtccactact tcggcgcaga ggacgaggcc gcggggtttc
     2701 aggtcaccat ggcgctcttc gccgtgatct cggtggtgat gttctggtac acgttcggcg
     2761 ctacccgcga gcgcgtgcgt tccctgcccg aaaccgacgt cagcttcggc aacgacgtga
     2821 agctcctgct cagcaaccga ccctggatcg tgctcttctt cgcagggatc ttcacgctgt
     2881 ccagcatagc catcaagaac gccgtcacga ttcactactt caagtactac gtcggcgacg
     2941 acagctcccc catcctgctc ttcttcgaca tcaccacgct cttcatgacc atcggcatgc
     3001 tggcgctcat cgctggggtc gcctgcacca agtggctgac ggcccgtttc gagaagaaga
     3061 ccctgctgat cgctctgtct cttggcaacg ccgtgaccat ggtcgccttc ttcttcgtcc
     3121 cgccggacca gctcgcgctg atgctcgcgc tcaacatcac cggttcgctc atggcgggac
     3181 ccacggcccc gctggtctgg gccatgtacg ccgacgtggc cgactacggc gagtggcgct
     3241 ttgagcgacg caccaccggg ctcgttttct ccgcttccat gttcgcgcag aaattcggcc
     3301 tgaccatcgg cgccgggctg gcaggctgga tgctcgcgct tttcggatac gtagccaacg
     3361 tcacccagag cgacgattcg ctgatgggta tccggctcat gttcaccctg ttccccgcgg
     3421 cggtgagcct gctgaacgtg gccgtgatcg ccttctacac cttgagcgac gccgaggtgg
     3481 aatccatcgg ccgggagctc gaggaccgga aggcacaggc ggggcctgct ccgatctgag
     3541 cctcggtggc agccatcggc gcctctggca taatcctgag ttggggtttc cggggcggga
     3601 tccgtggcag aagtatcaga acagatgccg cagctgttca tctcatactc tagagatgac
     3661 ggcagagccg tatatccaga cgcggattgg cttggcggtc tcggatatgc cgttcatatc
     3721 gacagacaca tcagtcccgg agagcaatgg cgcgaggcga tcgcgaacgc catcgcggac
     3781 agtgatctgc tgatctttta cgcctccccg tcctccatcg agtccaaatt ctgctcccag
     3841 gaattacagt tcgctctgag ccgcgacatt cccgtcataa cggtcatgct ggaagaaacc
     3901 ccgtttc
//
LOCUS       DBNHKV010000217         2319 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1589544, whole genome shotgun sequence.
ACCESSION   DBNHKV010000217 DBNHKV010000000
VERSION     DBNHKV010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1589544"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="AC16PP_06915"
     CDS             complement(<1..597)
                     /locus_tag="AC16PP_06915"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003626.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HIR6481395.1"
                     /translation="MAVLSVNLNKVALLRNARGADQPSVTGAAKTVLAAGAHGITVHP
                     RPDQRHVRPDDVGDLADLLRAYPGIEYNIEGNPFAGPRDNGYPGFDALIESARPHQAT
                     LVPDTDNQLTSDHGWDLTGDNGRLSDHINRYQAAGARVSLFMDPVPEHIARARDVGAD
                     RIELYTGPFAQTVAEFGLDHEESLASLDRYQKAAACAAE"
     gene            complement(601..1242)
                     /locus_tag="AC16PP_06920"
     CDS             complement(601..1242)
                     /locus_tag="AC16PP_06920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HIR6481396.1"
                     /translation="MALKSKSCLAALLMLAGLTLSARAETVYISDTFAVPLRSGPSNS
                     HRILHRGLPSGTELTVLERDDEGQFARVRTTRGTEGWIPQQYLVAEPIARDRLDEASR
                     EIERLSSLVAEREAEIAQLRQAGARTWEEIEAGAQPGNRGPAGASAASPGERRLMELN
                     ERLRHEFERLAAERDELKSSARQRWLLIGAGIMLAGIAIGVILKARPRRSGWS"
     gene            complement(1226..1786)
                     /locus_tag="AC16PP_06925"
     CDS             complement(1226..1786)
                     /locus_tag="AC16PP_06925"
                     /inference="COORDINATES: protein motif:HMM:NF016189.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane-spanning protein YciB"
                     /protein_id="HIR6481397.1"
                     /translation="MNQLLDFIPIVLFVAVFFLADIYYATAALMIAVTLQVLAYRLLK
                     KPIGRELALTFWASVIFGGLTLVFRNELFIQWKPTIVNWLLCASLLGSHFVGQRNLME
                     RMLGHQLTLVDQVWVRLNFGWAFGFFLAGALNLVVAYNFSMEFWVSYKLIGGFGLTLI
                     YIVITLVYLSKKGFLTDKPEGLGIEK"
     gene            1886..>2319
                     /locus_tag="AC16PP_06930"
     CDS             1886..>2319
                     /locus_tag="AC16PP_06930"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR6481398.1"
                     /translation="MSQYFVVHPSHPQRRLLVTAAQIISGGGLVVYPTDTAYALGCHI
                     GDKQSLESIRRIRRLDKRHHFTLTCRDLSEISLYARVSDANYRILKHLTPGPFTFLLP
                     ATREVPRRLVHPKRRTIGLRIPNNVIALELLAVLGEPMMTTT"
BASE COUNT          400 a          745 c          756 g          418 t
ORIGIN      
        1 ctcggcggca caggccgccg ctttctgata cctgtccagg ctggcgaggc tttcctcatg
       61 atccagcccg aactctgcca cggtctgtgc gaacggaccg gtgtagagct cgatgcggtc
      121 cgccccgacg tcgcgggcgc gggcgatatg ctctggcacc gggtccatga agagacttac
      181 ccgggcgccg gcggcctggt agcgattgat gtggtcggat agtcggccgt tgtcgccggt
      241 gagatcccac ccgtggtcgg aggtcagctg gttgtcggtg tcgggcacca gggtcgcctg
      301 gtggggccgg gcactttcga tgagcgcatc aaaccccgga tagccgttgt ctcggggacc
      361 ggcgaagggg ttgccctcga tgttgtactc gataccgggg tatgcccgca gcaggtcagc
      421 caggtcgccc acgtcgtcgg ggcgcacgtg acgctggtcc gggcgtgggt gcacggtgat
      481 gccatgggcg cccgcggcaa gcaccgtctt cgctgctccg gtgacgctgg gctggtcagc
      541 accgcgggcg ttgcgcagca gggccacctt gttcaggttt acgctcagaa ccgccaagga
      601 tcagctccag ccgctgcgac ggggacgggc tttgaggatc accccgatgg cgatgcctgc
      661 cagcatgatg ccggctccga tcagcagcca gcgctggcgc gcgctgctct tcagctcgtc
      721 acgctcggcg gccagccgtt cgaactcgtg gcgcagacgc tcgttcaact ccatcaggcg
      781 ccgctcgccg gggctggcag cgcttgcgcc tgcagggccg cggttgccgg gctgggcgcc
      841 ggcctcgatc tcctcccagg ttcgcgcccc tgcctggcgc agctgggcaa tctcggcttc
      901 ccgctccgcg acgagactgc tcagccgctc gatctctcgg ctcgcctcgt cgagccgatc
      961 ccgcgcgatg ggctcggcaa cgaggtattg ctgcgggatc cagccttccg ttcctcgggt
     1021 ggtccgcacc cgggcgaact ggccctcgtc gtcccgttcc agcacggtca gctccgttcc
     1081 gctggggagc ccccggtgga gtatccgatg cgagttcgac ggtccgcttc gaagcgggac
     1141 cgcgaaggtg tcggagatgt agaccgtctc ggcccgggcc gacagggtga ggccggcgag
     1201 catgagcagg gctgccaggc aggatttact tttcaatgcc aaggccctct ggcttatccg
     1261 tgagaaaacc tttcttgctc aggtatacca gcgtgatgac gatgtagatg agcgtgaggc
     1321 cgaaaccccc gatcagcttg tagctcaccc agaactccat ggagaagttg tacgccacca
     1381 cgaggttgag cgcgccggcg agaaagaagc cgaacgccca gccgaagttg agtctcaccc
     1441 acacctgatc caccaaggtc agctggtgac cgagcatgcg ctccatgagg ttgcgctgcc
     1501 ccacgaagtg gctccccagc agcgatgcgc acagaagcca gttgacgatg gtcggcttcc
     1561 actgaatgaa cagctcgttg cggaaaacca gggtgagccc gccgaatatg acgctcgccc
     1621 agaaggtcag cgccagctcg cggccaatgg gtttcttcaa aaggcgatag gcgagaacct
     1681 gcagggtcac ggcgatcatg agcgctgccg tggcgtagta gatgtcggca aggaagaaaa
     1741 cggccacgaa cagcacgatg gggatgaagt cgagcagctg attcatcgtt gattggccga
     1801 atagccccgc tgcgcgcggg gatcctggat aacgctaagc gtgtgataat acgagaaacc
     1861 gagtgggcat ggtaagcagg ggtctgtgag tcagtatttc gtcgtgcatc cttcgcaccc
     1921 gcagcggcgg cttctcgtca ccgcggctca gatcatctcg gggggcgggc tggtcgtcta
     1981 tccgaccgat acggcctacg cgctcggatg ccacatcggc gacaagcagt cgctggagag
     2041 cattcgtcga atccgccgcc tggacaagcg tcatcacttc accctgacct gccgggatct
     2101 ctcggagatc tcgctgtacg cccgcgtttc ggatgcgaac taccgcattc tcaagcacct
     2161 caccccggga ccgttcacgt tcctcctgcc ggcgacccgg gaggtcccac gtcggctggt
     2221 tcacccgaag cggcgcacga tcggcctgag aatcccgaac aacgtcatcg cgctcgaact
     2281 gctcgccgtg ctcggcgagc ccatgatgac gaccaccat
//
LOCUS       DBNHKV010000218         3596 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_243503, whole genome shotgun sequence.
ACCESSION   DBNHKV010000218 DBNHKV010000000
VERSION     DBNHKV010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_243503"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..784)
                     /locus_tag="AC16PP_06935"
     CDS             complement(11..784)
                     /locus_tag="AC16PP_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481399.1"
                     /translation="MKRIILALSLMTAPLLAGAEEAAPTPMYVAATNLTVSGHPGAQN
                     RFETFIKRAAEAHAKLDTKRHWFAWQQEMGNPRSYTLARTFGSMTAMDETPMNPIAEA
                     FGPEEIAKLTPDHEVVASVERGVWVQRPDLAVMPDSPVGQRVIWLQATVRAGENERFE
                     AYVAKVAEATREVAPEVRYATFAPVFGSQNTYVFSVPTSYAQLDEAPVMSVSERLREA
                     FGEREARRLESERAATVESLVQLLVRPRPDLAYQPPAEE"
     gene            954..1766
                     /locus_tag="AC16PP_06940"
     CDS             954..1766
                     /locus_tag="AC16PP_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6481400.1"
                     /translation="MATPANQIDTDEVLYDVADHVATITINRPDAQNTISGPMLDALT
                     QRLLEANENNDVRAIIVTGSGRFFCAGLDLRGGGIAGGLSSQGAGPANLDLRNTPPTV
                     LHSLDTPTICALNGSAAGYGMDLALGCDIRIMADSAKMAAAFTARGVVPESGGTWILP
                     RLLGWSKAAEIIFTGRTLLADECKELGLVSHVVPADDIGDAARNLALEIAANAPLAVQ
                     ASKRMMRMGMNETFNDHVHHVYLQLLPLFRSEDFREGMASFMEKRKAEFKGQ"
     gene            1957..2033
                     /locus_tag="AC16PP_06945"
     tRNA            1957..2033
                     /locus_tag="AC16PP_06945"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1991..1993,aa:Pro,seq:ggg)
     gene            complement(2437..2748)
                     /locus_tag="AC16PP_06950"
     CDS             complement(2437..2748)
                     /locus_tag="AC16PP_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481401.1"
                     /translation="MGIQDLGAIGEFVSSLVILFTLLVLIYEVRGAKNATLQANAQER
                     QRKQEGLLTTMSQTPSLMASWAKVNQHLGDTRWSEEAAQFGLASDEWRQLTVHFARMM
                     A"
     gene            complement(2831..3343)
                     /locus_tag="AC16PP_06955"
     CDS             complement(2831..3343)
                     /locus_tag="AC16PP_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481402.1"
                     /translation="MTIAELGSIGEFVGSLGVIATLFYLAVQIRTNTKTTRASASFDA
                     IHSWAQLNENIAIQPDEFHAIMLQASDDSTKADVFSETEWQKISLLNRAVMQKLEGQY
                     YLYKYGLMEPDIWVKRSSAGRAYIEPPAQRQWWEEEKSIRTFTEAFVAAIESAEKLAV
                     PGSAINRRTG"
BASE COUNT          688 a         1131 c         1025 g          752 t
ORIGIN      
        1 cggctgggtg ctactcctcc gccggtggct gataggccag gtcgggtctg ggtcggacca
       61 gcagctggac cagagattcc acggttgccg ctcgctccga ctcgagacgt cgcgcctcgc
      121 gctcgccgaa cgcctcgcga agccgctccg atacggacat gacaggggct tcgtccagct
      181 gggcatagct ggtgggcaca gagaagacgt aggtgttctg ggacccgaag acgggcgcaa
      241 aggtcgcgta gcgaacctca ggcgccactt cccgggtggc ttccgccacc ttggccacgt
      301 aggcttcaaa gcgctcgttc tcgcccgctc tcaccgttgc ctgcagccag atcacccgct
      361 gccccactgg cgaatccggc atgacggcca gatcgggccg ttgcacccaa acacccctct
      421 cgacgctggc cactacctca tggtcgggcg tgagcttggc aatttcttcc gggccgaaag
      481 cctcggcgat cgggttcatc ggcgtctcgt ccatggcggt catgctgccg aacgtgcgcg
      541 ccagcgtgta ggagcgcggg ttgcccatct cctgctgcca ggcgaaccaa tgccgtttcg
      601 tgtccagctt ggcgtgggct tcggccgccc gcttgatgaa ggtctcgaaa cggttctgcg
      661 cgccgggatg accgctcacc gtcaggttgg tagcggcaac gtacatgggc gtcggcgcgg
      721 cctcttccgc acccgccagc agcggtgccg tcatcaggct cagcgccaga attattcttt
      781 tcatgatcgc tcgtcctcgt atgctttggt tgtgtcagtc cgggtgcaaa cacgaccacc
      841 acctgcaaac ggcgcgcatc tgcccaacta tcccggagct tagcaccaac ttgcgggata
      901 gagatcgaaa gattagtttg cacacctgac gaaccgttcg gggaaagaaa gcaatggcca
      961 cgcctgccaa ccagatcgac actgacgagg tgctctacga cgtggcagat cacgtcgcca
     1021 ccatcaccat caaccgtccg gacgcacaga acaccatctc cgggccgatg ctcgacgcgc
     1081 tgacgcagcg cctgctcgaa gccaacgaga acaacgacgt tcgagccatc atcgtgacgg
     1141 gaagcggtcg cttcttctgc gcgggtctgg atctgcgcgg cggcggcatc gccggcgggc
     1201 tttcttccca gggagcgggg cccgccaacc tcgacctgcg caacacgccg ccgacggttc
     1261 tccacagcct ggacaccccc accatctgcg cgctgaacgg ctccgcggcc ggctacggga
     1321 tggatctggc gctgggctgc gacatccgca tcatggcgga ttctgccaag atggccgccg
     1381 ctttcaccgc acggggcgtg gttcccgaat caggcgggac ctggattctg ccccgtcttc
     1441 tgggctggtc gaaggcggcc gagatcattt tcacggggcg caccctgctc gctgatgaat
     1501 gcaaggagct cggactggtc tcccacgtcg tgcccgccga cgacatcggg gacgcggccc
     1561 gcaacctggc actcgagatc gccgccaatg cgcctctggc cgtgcaggcc tccaagcgga
     1621 tgatgcggat gggcatgaac gagacgttca acgaccacgt ccaccacgtg tacctgcagc
     1681 tgctgccact tttccgctcc gaggatttcc gcgaagggat ggcttccttc atggagaagc
     1741 gcaaggccga gttcaaggga cagtagccgc ctcgcgcccg ggcgcatgct ctttgcgata
     1801 cgcccacaag tccaggtgcg tctcgggtgg ggcggggtag cagccgtcaa tcgttcgctt
     1861 ttacggctcc tacccttcag gtatcatttc gcgccctctg gcctcggatt gcgcaccaca
     1921 gatcgcaccg ggccaggcgg ataaatccag acctttcggg gcgtagcgca gtctggtagc
     1981 gcacttgact gggggtcaag tggtcgcagg ttcaaatcct gccgtcccga ccaacaacac
     2041 ccctgaccga caccgcaacc gataccaacg tatttcggcg aatgatcccg tgcaaaccgg
     2101 ggccgacacc ctagcctgcc gacttcctaa actctcagca cctcaggcga cacatgattt
     2161 ccatgcgcca tctgcctctg tggacttcag acgttaagtg gtgggactgc cgggagaggt
     2221 ttgtacctac atcgcttgtg ctttctctat tgtcgcttgc acgttggccc ggatcttatc
     2281 gacgtgacgg atgaacgaat tatctcgccc ccaattcggt gcagcgattt catcgtagta
     2341 tttcccgaac gttgcatctg acaacaacgt aaccgttacg aagtcgttga ccctgcgctc
     2401 gacctccgga aggtccgatg agaaagcctc ccgccattac gccatcatgc gagcgaagtg
     2461 cacagtcagt tgccgccact catccgaggc aagaccgaac tgggcagctt cttccgacca
     2521 acgggtgtca ccaagatgct gattgacctt ggcccaagac gccatgaggc tgggggtctg
     2581 ggacatggtg gtcagcaacc cctcctgttt gcgctgccgc tcctgcgcat tggcttgtag
     2641 ggtcgcattt ttcgcgcccc gtacttcgta gatcagcacc aagagcgtga acaagatgac
     2701 tagcgagctg acgaactccc cgatggcccc gagatcctga atacccattg gacgtctccc
     2761 gttgtggatg acgggagttt agtccaaccg caatcacgca tggtgtcctg aactagtggc
     2821 tcgttggcac tcatccggtt cttcggttga tcgctgagcc cggaactgca agcttctcag
     2881 cggactcgat ggcggctaca aacgcttccg tgaacgtcct tattgacttt tcctcctccc
     2941 accactgccg ctgagccggt ggttcaatat acgctcggcc tgcggaactg cgcttgaccc
     3001 agatgtcggg ttccatcaga ccgtacttgt acagatagta ttggccttcg agcttttgca
     3061 tcaccgctcg attaagcagc gaaattttct gccactcggt ctcagaaaag acatctgcct
     3121 tcgtggagtc atcgctggcc tgcagcatga tggcgtgaaa ctcgtctggt tggatcgcta
     3181 tgttctcatt gagttgtgcc caactgtgga tcgcgtcgaa gctggcactg gcccgtgttg
     3241 ttttcgtgtt cgttcggatt tgaacagcga gataaaacaa cgtcgcgatc acacccaacg
     3301 aaccaacgaa ctcgccgata gatcccagtt ctgcaatcgt catctgaagc ctcctctcca
     3361 atgaatggaa cgaggctacc tcagccgatc tctgccgcaa gtcttctgcg ctaccattct
     3421 ccatctaccg gaggaggtga gttatggata ttcagggtgt agcgcagttg ctgggcaact
     3481 ttggggagtt ctttggggcg atagccgttc tagcaacgct tgtgtatctg gtcattcaga
     3541 tcaagcagaa caacaagtta ttgacggcaa ccgtctatga atccgcaatg gagggt
//
LOCUS       DBNHKV010000219         8426 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1519393, whole genome shotgun sequence.
ACCESSION   DBNHKV010000219 DBNHKV010000000
VERSION     DBNHKV010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8426)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1519393"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="AC16PP_06960"
     CDS             <1..480
                     /locus_tag="AC16PP_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like/CBS
                     domain-containing protein"
                     /protein_id="HIR6481403.1"
                     /translation="AFRQRSVFISTLKTLGLEYPPSPVTAHMQRAGVAAIMCEEVAVL
                     PRDCSLDAARRALEQRPLWIAIASDDGETRWVLKSSDLAAHLESEQIDTADVDLLNIP
                     GMRRDATSVDVRCTVDEAQMALQGSPAEALCVRRLGVTAAGSLLGIVTQEDIDTYRG"
     gene            489..923
                     /gene="hemJ"
                     /locus_tag="AC16PP_06965"
     CDS             489..923
                     /gene="hemJ"
                     /locus_tag="AC16PP_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007251893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HIR6481404.1"
                     /translation="MSAGYLWLKALHIIFVVTWFAALFYLPRLFVYHSSSTDAISRDR
                     FKVMESKLYRVIMRPSMLLATGFGLWLVFLGWNAFAGSGWLWVKLTAVVALLGYHHYC
                     ARLVRAFAADADPHSETFYRWFNELPALVLIVVVIMVVVKPF"
     gene            951..2231
                     /gene="hemL"
                     /locus_tag="AC16PP_06970"
     CDS             951..2231
                     /gene="hemL"
                     /locus_tag="AC16PP_06970"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HIR6481405.1"
                     /translation="MHTDRSRELMRRAALSIPGGVNSPVRAFKGVGGSPIFFERGEGA
                     FLTDVDGNRYIDYVGSWGPMLLGHGNPEVVAAVRAQAEKALGFGAPTELEIELAEQVV
                     DMVPSIEKIRMVNSGTEATMSAIRLARGFTGRDLIVKFDGCYHGHSDSLLVKAGSGVL
                     TLGIPNSPGVPAAIAEQTLTLPFNDLDAVAEAFEQYAGQIAAVILEPVAGNMGCVPPE
                     AGYLEGLRELTGRHGSLLIFDEVMTGFRVSRGGAQQRYGVEPDLTTLGKIIGGGLPVG
                     AFGGRADVMAHIAPEGTIYQAGTLSGNPLAMVAGLTTLRALDDPLYEELEQRTAYFCE
                     ALEASANRHGVSVTINYVCGMFSVFFTNGPVRNFADVARSDTASFNRFFHAMLDGGVY
                     LAPSAFEAGFLSVAHTEDVLDATIQAADQAFATL"
     gene            2246..3241
                     /locus_tag="AC16PP_06975"
     CDS             2246..3241
                     /locus_tag="AC16PP_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HIR6481406.1"
                     /translation="MYDIYGLGNALVDMEYRVDDGFLRRHAITKGHMTLVEEDRLDAL
                     IADLDDYDPERMSGGSAANTVIAAQCFGVSTFYSCKVAGDATGHYFLRDMGTAGVNTN
                     ANANDGPGKSGRCLVLVTPDAERSMNTFLGVSSQLSTAEIDEAALAKAGFFYVEGYLS
                     SSPGSLEAAVECRQLAEDSGVKTAVSLSDPSMVEFFRDGMEQILGNGVDHLFCNEEEA
                     LAWSRTDRLDIAIRELAEVARAVNITLGARGSLAVNGREQRRAPGYPVTAVDTNGAGD
                     IYAGACLFGWAAGMSSEQAAAFGNFAAANLVQRYGARFRHAGDYQSVLGDFRKVS"
     gene            complement(3234..3935)
                     /gene="sfsA"
                     /locus_tag="AC16PP_06980"
     CDS             complement(3234..3935)
                     /gene="sfsA"
                     /locus_tag="AC16PP_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HIR6481407.1"
                     /translation="MNFHPPLEVARLERRYKRFLADLLTDQGPLTVHCPNTGAMLGCS
                     DPGSEVWYSDSGNERRKYRHTLEVVCTPTGRVGINTARANRLVEEAIARGGVAELDGY
                     GQIRREAAIPESKGRFDFLLEGARGRCFVEVKSMTHNLGRGRGAFPDTVSERALRHVE
                     ALIGRKRAGDRAVLLFCVQHTGIDLATTADAIYPEYGEAVRRAVSEGVEVIAYGCRID
                     RDALAVGEALPVELS"
     gene            complement(3932..5104)
                     /locus_tag="AC16PP_06985"
     CDS             complement(3932..5104)
                     /locus_tag="AC16PP_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007159703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6481408.1"
                     /translation="MYAQRLDRIAPFRVMQLMERAKAIEASGHRVVHFEVGEPDFVTA
                     DPIVRAGIDALEAGETKYTQALGIPELRERIAQHYCQELGVSLSADRVVVTSGASGGL
                     LLLSGLLLNPGDRLLLTDPGYPCNEVFAGLVNAEPERIPLSADRGFQLHADDLEAHWG
                     PRARGLLLASPANPTGAVISRAHLAELVAALARRGGWLILDEIYQGLIYGDGPGYRTG
                     LEITDEAFILNSFSKYYGMTGWRLGWMVVPEAALEPVARLAQNLFISPSSIAQRAALS
                     AFGPEARAIHEQRRAAFEGRRDALREGLTELGLRVPVVPGGAFYLYVDVSPIGIDSAE
                     FCWRLMDDYHVAVTPGIDFGDHLADQYVRFAYTTDEDSIALGLERIETALKDMGAL"
     gene            5268..5729
                     /gene="dksA"
                     /locus_tag="AC16PP_06990"
     CDS             5268..5729
                     /gene="dksA"
                     /locus_tag="AC16PP_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HIR6481409.1"
                     /translation="MAARAKNRSTKAKAVDSPFRNFTPYKPRKSEAYMSDGQVEHFRN
                     ILSKWRQELMEEVDRTVHHMQDEAANFPDPADRATQEEEFSIELRTRDRERKLIRKID
                     QTLDRLDQGDFGYCDTCGVEIGLRRLEARPTANQCVDCKSLDEIKERQLHG"
     gene            5765..6622
                     /gene="gluQRS"
                     /locus_tag="AC16PP_06995"
     CDS             5765..6622
                     /gene="gluQRS"
                     /locus_tag="AC16PP_06995"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009050893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HIR6481410.1"
                     /translation="MAGRFAPSPTGPLHLGSLLAATASYLDARAAGETWRLRFDDLDV
                     PRNDPGAIGSITATLRAHGLIWDGPIVHQSDNLTAYEEALNRLDRMGLLFYCTCSRRD
                     LRAEAVYPGTCRAFREPRRDSATRVLVDQVAIEFADRIQGPQRCRLDQTVGDFIVRRR
                     DGLVAYQLATAVDDGGEEVTSVVRGADLLDNTPRQIYLMQRLGLKPPRYAHIPVLLKP
                     DGSKLSKQTMAPPVDAHRAPENLLTAYGLLGMRPPSDAQRWEVERILSWGISHWDQEK
                     VPRRRSVEP"
     gene            6678..7997
                     /locus_tag="AC16PP_07000"
     CDS             6678..7997
                     /locus_tag="AC16PP_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR6481411.1"
                     /translation="MYHVLLVDSGNRRETVRGLLENAGITVVGPESIEALRQIDARQY
                     RCLLAAVGPLFGTTEDAIDLAGRLPVVLLDPVGDARRAVAAMKRGAADYLVCPFTEEE
                     LLSAVERCRPTGAPQPRDPLNMVGASQPMKDLYNRIAQVAPTDSTVLIEGESGSGKEL
                     VARAIHAASRRNDAPLITLNCATVPAELIESELFGRPAEGQAASRHGLLEAANGGTLF
                     LDEIAELPPNVQARLLRVLQDGCLGLSSDGTPFTVDVRIIAATHQNLRQLIEADQFRE
                     DLYYRLNVVNLPIPPLRARGDDVLQLAESILRRTCKRLSRTCPGFTRSAQEAMQQYAW
                     PGNVRELENAVERAVILCSESAIEPHLLAIDLTPPASVEPPPSMNDRSSLEDYFVRFV
                     TAHQDQLTETELAEKLGISRKSLWERRQRLNIPRKKTRKRGPRRDST"
     gene            7994..>8426
                     /locus_tag="AC16PP_07005"
     CDS             7994..>8426
                     /locus_tag="AC16PP_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020293215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HIR6481412.1"
                     /translation="MSEPVRLDATEHGLLRDQVDPEALDVTRRLSEAGYQALLVGGCV
                     RDLLLGAEPKDFDVATDATPEEVRQLFRRSRLVGRRFRIAHVRYGRKVIEVSTFRRSH
                     TEDEDERRHSEEGVILRDNLYGSLEEDAFRRDFTINALYYDP"
BASE COUNT         1549 a         2807 c         2685 g         1385 t
ORIGIN      
        1 gcgttcaggc agcgatcggt gttcatctcc accctgaaga ccctggggct cgagtacccg
       61 ccaagcccgg tcacggcgca catgcagcgc gccggcgtgg cggccatcat gtgcgaagaa
      121 gtggcggtgc ttccccgtga ctgctctctc gacgccgcgc gaagggcact cgagcaaagg
      181 ccgttatgga ttgcgatcgc atcggacgac ggcgagaccc gctgggtcct caagtcgagc
      241 gacctggccg cccacctaga atccgagcag attgacaccg ccgacgtcga cctgctcaat
      301 attcccggca tgcgtaggga cgcgaccagc gtcgacgtgc gctgcaccgt ggacgaagcc
      361 cagatggccc tgcagggaag ccctgccgaa gcgctgtgcg tgcgccggct gggcgtgacg
      421 gcggctggga gcctgctggg catcgtcacc caggaagaca tcgacaccta cagaggttga
      481 tccaaacgat gagcgccggc tacctgtggc tgaaggccct tcacatcatc ttcgtcgtga
      541 cctggttcgc ggccctcttc tacctgccga ggctgttcgt ctaccactcc agcagcaccg
      601 atgcgatcag ccgcgaccga ttcaaggtga tggagagcaa gctctaccgc gtcatcatgc
      661 ggccctccat gctgctggca accggcttcg gcctctggct tgtcttcctc ggctggaacg
      721 cgttcgccgg ctcgggctgg ctgtgggtga aactgactgc ggtggtcgca ctgctcggtt
      781 atcatcatta ctgcgcgcgg ctcgtcagag cctttgcggc agatgccgac ccccacagcg
      841 agacgttcta ccggtggttc aatgagctgc ccgcactcgt gctgatcgtg gtggtcatca
      901 tggtggtggt caaacccttc tagctcccgt cacgcgatag gggtcgagcg atgcacacag
      961 acaggtcgag ggaactgatg cggcgggcgg ccctgtccat tcccggcggc gtcaactccc
     1021 ccgtccgcgc gttcaagggc gtcggcggct cgcccatttt cttcgagcgg ggtgagggcg
     1081 cgtttctcac agacgtggac ggcaaccgtt acatcgacta cgtcggctcc tggggcccga
     1141 tgttgctggg gcatggcaac cccgaagtcg tcgccgccgt gagggcccag gccgagaaag
     1201 ctctgggctt cggcgcgccc accgagctcg aaatcgagct cgcggagcag gtcgtggaca
     1261 tggtgccgag catcgagaag atacggatgg tgaactcggg caccgaggcc accatgtcgg
     1321 caatccggct cgccagaggc ttcaccgggc gcgacctcat cgttaagttc gacggctgtt
     1381 atcacgggca ttccgactcc ctgctggtca aggcgggctc gggcgttctg acgctgggca
     1441 tcccgaactc acccggggta cccgcggcca tcgccgagca gacgctgacg ctgccattca
     1501 acgatctgga cgcagtagcc gaagcgttcg aacaatacgc tgggcagatc gctgcggtca
     1561 ttctcgagcc ggtggcggga aacatgggat gcgtaccgcc ggaggcaggc tacctggaag
     1621 ggctccgcga gctgacgggc cgccacggca gcctgctcat cttcgacgag gtcatgaccg
     1681 gatttcgcgt gtcgcgcggc ggcgcccagc agcgctatgg cgtggagccg gatctgacca
     1741 cactcggaaa gatcatcggc ggggggctgc cggtgggcgc gttcggcggg cgcgccgacg
     1801 tcatggcgca cattgcgcct gaaggcacca tctatcaggc gggaacgctc tcgggcaacc
     1861 cgctggcaat ggtggcgggc ctgacgacct tgcgcgcgct cgacgatccc ctctacgagg
     1921 aactggagca gcgcacggcc tatttctgcg aagccctgga agcatcggca aatcgccacg
     1981 gcgtgagcgt aaccatcaat tacgtgtgcg gcatgttcag cgttttcttc accaacggcc
     2041 cggtcaggaa cttcgcggac gtggcccgtt ccgacacggc atctttcaac cggttctttc
     2101 acgccatgct cgacggcggc gtctacctgg caccctcggc tttcgaggcc ggcttcctgt
     2161 cggtcgccca cacggaggac gtgctggacg ccacgatcca ggccgcagat caggcgttcg
     2221 caacgctgta aggggagccc cgttcatgta tgacatctac ggcctgggaa atgccctggt
     2281 ggacatggag tatcgcgtgg atgacggttt tctgcgccgt catgccatca ccaaaggaca
     2341 catgacgctg gtggaggaag accggctcga tgctctgatc gccgacctgg acgactacga
     2401 ccccgaacgc atgagcggtg gatccgcggc aaacacggtc atcgcagccc agtgcttcgg
     2461 cgtaagcacc ttctactcct gcaaggtcgc cggcgacgcc accggccact actttctgcg
     2521 ggacatgggc acggcgggcg tgaacaccaa cgccaatgcc aacgacggac cgggcaagtc
     2581 ggggcgttgc ctggtgctgg tcacacccga cgccgagcga tccatgaaca cctttctcgg
     2641 cgtgtcgagc cagctcagca cagccgagat cgacgaggcg gcactggcca aagcaggctt
     2701 cttctacgtc gagggctacc tgagctccag tccgggaagc ctggaggcgg ccgtggagtg
     2761 ccggcagctt gccgaggact cgggcgtgaa aaccgcggta agcctctccg atcccagcat
     2821 ggtcgagttc ttcagggacg gcatggagca gattctgggc aacggcgtgg accatttgtt
     2881 ctgcaacgag gaagaagcac tggcgtggtc gcgaacggac cggctggaca tcgccatccg
     2941 cgagctcgcc gaggtggccc gggcagtgaa catcaccctg ggcgcccggg gcagcctggc
     3001 cgtgaacggc cgtgagcagc gccgcgcgcc gggctacccg gtgacggccg tcgacaccaa
     3061 cggtgccgga gacatctacg ccggtgcctg cctgttcggc tgggcggccg gcatgtcctc
     3121 ggagcaggcg gccgccttcg gaaacttcgc ggcggctaac ctggtacagc ggtacggtgc
     3181 ccgattcagg cacgcaggcg actaccagag cgtgcttggc gacttccgga aggtcagctg
     3241 agctccacgg ggagcgcctc gcccaccgca agggcatccc gatcgatccg acacccgtag
     3301 gcgatcacct cgacaccctc ggagacggcc cgacgcaccg cttcaccgta ctcgggatag
     3361 atcgcatccg ccgtggtcgc caggtcgatg ccggtgtgct ggacgcagaa cagcagcacg
     3421 gcgcggtcac cggcccgctt cctgccgatc agcgcttcga cgtggcgcaa cgcccgttcg
     3481 ctcaccgtat ccgggaaggc gccgcgcccc cggccgagat tgtgggtcat gctcttgacc
     3541 tcgacgaagc agcgccccct ggcgccctcg aggaggaaat cgaagcgacc cttgctttca
     3601 gggatggccg cttcgcgcct gatctgcccg tacccgtcaa gctcggccac gccgccccgg
     3661 gcaatggctt cttcgaccag ccgattggct cttgccgtgt tgatccccac ccggccggtc
     3721 ggcgtgcaca ccacctcgag cgtgtgacga tacttgcggc gctcgttccc ggagtctgaa
     3781 taccagacct cgctgccggg atctgagcat ccaagcatgg cgccggtgtt ggggcagtgc
     3841 acggtcagcg ggccctggtc ggtcaacagg tcggcgagaa atcttttgta gcgacgctcc
     3901 aggcgagcaa cttccagcgg cgggtggaaa ttcacagggc gcccatgtcc ttgagcgccg
     3961 tctcaatccg ctcgagcccg agggcgatgg agtcttcgtc ggtggtgtac gcgaaccgca
     4021 cgtactgatc ggccagatgg tcaccaaaat cgatccccgg cgtaaccgct acgtggtaat
     4081 cgtccatcag gcgccagcag aactcggccg agtcgatgcc gatgggcgag acatccacgt
     4141 agagatagaa cgccccgccc ggcaccacgg ggactcgcag accgagctcc gtcaggccct
     4201 cgcgcagcgc gtctctgcgc ccctcgaagg cagcacgtcg ttgttcgtga atcgcccgcg
     4261 cctccggccc gaaggccgac aatgccgccc gctgggcgat ggacgacggc gagatgaaga
     4321 gattctgggc gagcctggca acgggctcca gcgcggcctc cggaaccacc atccacccga
     4381 gccgccagcc ggtcattccg tagtacttgg aaaagctgtt gagaatgaat gcctcgtcgg
     4441 tgatctcaag accggtgcgg tatccgggcc cgtcaccgta gatcaggccc tggtagatct
     4501 cgtcgaggat cagccagccg cctctacgag ccagggcagc aacgagctcc gcgagatgcg
     4561 cccgggagat caccgccccc gtggggtttg ccggcgaagc cagcagcaaa ccccgcgctc
     4621 tgggccccca atgcgcctcc agatcgtcag cgtgcagctg aaagccacgg tcagcggaca
     4681 gcggaattcg ctcgggctcg gcgttgacga gcccggcgaa cacctcgttg cacggatatc
     4741 cggggtcggt gagcagcagg cggtcacccg ggttcaacag caacccgctc agcaggagca
     4801 gaccaccgct ggcgcccgag gtgaccacga cccggtcggc ggagagagaa acaccaagtt
     4861 cctgacagta atgctgggcg atgcgctcac gcagctccgg gatgcccagc gcctgggtgt
     4921 actttgtttc ccctgcctcc agggcatcga tgcccgcccg aacgatgggg tcggcggtca
     4981 cgaagtccgg ctcgcctacc tcgaagtgca cgacgcggtg ccccgaagcc tcgatggcct
     5041 tcgcccgttc catcaattgc atgacccgaa acggggctat tcggtccagc cgctgcgcgt
     5101 acatgtgccc tgaattctcc ctttgagccg tgcgtcagaa atcacgaaaa acctggacgg
     5161 attatatggt caacgccgat ttcttctggt agcttacgcg cccttcgcga aatccggctc
     5221 gaaaaatcag ccgattttcc attggaaagg acgatcagag gcgacatatg gcggcccgcg
     5281 caaaaaatcg atctacgaaa gccaaggcag tcgattctcc atttcgcaac ttcacgccct
     5341 acaaacccag gaagtcggaa gcgtacatga gcgacggcca ggtcgagcac ttccgcaaca
     5401 tcctctccaa gtggcgacag gagctgatgg aagaggtcga tcgaaccgtc caccacatgc
     5461 aggacgaagc ggccaatttc ccggatccgg cggaccgcgc gacccaggaa gaggaattca
     5521 gcatagagct ccggacccgc gacagggaac gcaagctaat caggaagatc gaccagaccc
     5581 tcgatcgcct ggaccagggt gacttcggct actgcgacac ctgcggggtc gagatcggtc
     5641 tgcgccgctt ggaggcacga cccaccgcca accagtgcgt cgactgcaag tccctcgacg
     5701 agatcaagga gaggcagctg cacggctgac gggacgctcc gtctcccgcg catcttcgca
     5761 aggcatggcc ggtcggttcg cgccgtcacc aaccggtccg ctgcacctcg gatcccttct
     5821 cgccgcgacc gccagctacc tggatgcccg tgccgccggc gaaacctggc ggctgcgctt
     5881 cgacgatctg gacgttccca gaaacgatcc cggcgccatt gggagcatca ctgccacgct
     5941 cagggctcac ggcctgatct gggacgggcc catcgtccac cagagcgaca acctgacggc
     6001 atacgaagag gcgctgaacc gtctcgaccg catggggctg ctgttctact gcacctgttc
     6061 caggcgcgac ctgcgcgccg aggcggttta ccccggcacg tgccgcgcct ttcgcgagcc
     6121 ccgacgagac agcgccacca gggtgctggt ggaccaggtg gccatcgagt tcgccgaccg
     6181 catacaaggc ccgcaacgct gtcgtctcga ccagacggtg ggcgacttca tcgtgcgccg
     6241 acgcgacggc ctggtggcct accagctggc caccgcggtg gatgacgggg gcgaagaggt
     6301 tacttcggtg gtcaggggcg ccgacctgct ggacaacacg ccccgccaga tctacctgat
     6361 gcagcggctc ggtctgaagc cgccgcgcta cgcgcacatt cccgtcctcc tgaaacccga
     6421 cggcagcaag ctgtccaagc agaccatggc cccgcccgtg gacgcccatc gggcgcccga
     6481 gaacctgctg acggcgtacg gcctgctcgg tatgcgcccg ccttccgatg cgcaacggtg
     6541 ggaagtcgag cgcattctct cctggggcat cagccactgg gaccaggaga aggtcccacg
     6601 tcggcggtcc gtcgagcctt gacgccccac tgcagggtcg cgcgcgcacc ctgacctttt
     6661 tgttaccatc gcgcgccatg tatcacgtac ttctggtgga ttcgggcaat cgtcgcgaga
     6721 ccgttcgagg ccttctcgag aacgccggca tcacggtggt agggccggag agcatcgaag
     6781 cgctccgcca gatcgatgcc cgccagtacc ggtgcctgct cgccgctgtg ggccctctgt
     6841 tcggaacgac cgaagacgcc atcgacctgg ccggcaggct ccccgtggtg ctcctggatc
     6901 ccgtaggcga cgctcgccgg gcggtggcag ccatgaagcg cggcgccgcc gactacctgg
     6961 tctgcccctt cacggaagag gagctcctga gcgccgtaga gcgctgccgc cccacaggcg
     7021 ccccccagcc gcgtgacccg ctgaacatgg tgggcgcgag ccagccgatg aaggatctgt
     7081 acaaccggat cgcccaggtc gcgcccaccg actcaacggt gctcatcgag ggagagtccg
     7141 gctccggaaa ggagctcgta gcgcgcgcga tccacgcggc aagccgccgc aacgatgcgc
     7201 cgctgatcac gctgaactgc gccaccgtgc cggctgagct gatcgagtcc gagctgttcg
     7261 gcagaccagc cgaggggcaa gcggccagcc gccacgggct tttggaagcg gccaacggcg
     7321 ggaccctgtt tctggacgag atcgccgagc tgcctccgaa cgttcaggcg cgtctgctgc
     7381 gagtgcttca ggacggctgc ctgggcctgt ccagcgacgg tacccccttc accgtggacg
     7441 tgcgcatcat cgccgcgacc caccagaacc tgagacagct catcgaggca gaccagttcc
     7501 gggaggatct ctactatcgc ctgaacgtcg tgaatctgcc gattccgccg ttgagagcca
     7561 ggggcgacga tgtcctacag ctcgcagaga gcatcctcag gcgaacgtgc aaacgactct
     7621 cgaggacctg cccgggattc acccgatcgg cccaggaagc catgcagcag tacgcctggc
     7681 ccggcaacgt ccgagagctc gagaacgctg tggaaagggc cgtcatcctg tgctcggagt
     7741 ccgcaatcga accgcatctg ctggccatcg acctgactcc gcccgcttcc gtcgagccgc
     7801 cgccctccat gaacgaccga tccagcctgg aggactattt cgtgcggttc gtgaccgccc
     7861 accaggacca gctgacggag accgagctcg cggaaaagct ggggatcagc cgcaagagcc
     7921 tgtgggagag gcgtcagcgg ctgaacattc ctcgtaagaa gacccgcaag cggggtccgc
     7981 gcagggacag cacttgagcg agccggtcag gctagacgcg acggagcacg gtctccttag
     8041 agaccaggtg gatcccgagg ctctggatgt caccaggcgg ctgtccgagg ccggctacca
     8101 ggcccttctc gtgggcgggt gcgtcagaga cctgctcctg ggcgcagagc caaaggattt
     8161 cgacgtggca acggatgcaa cgccggagga ggttcgccag ctctttcgac gctcccggct
     8221 ggtcggccgc agatttcgca ttgctcacgt gcgctacggc cgcaaggtca tcgaggtatc
     8281 gaccttccgc cgctctcaca ccgaagacga ggacgagcgt cgtcactcgg aggaaggggt
     8341 cattctccgc gacaacctgt acggcagcct ggaagaggac gccttccgca gggacttcac
     8401 gataaacgcg ctctactacg atcccg
//
LOCUS       DBNHKV010000220         1929 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1376295, whole genome shotgun sequence.
ACCESSION   DBNHKV010000220 DBNHKV010000000
VERSION     DBNHKV010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1376295"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="AC16PP_07010"
     CDS             complement(<1..590)
                     /locus_tag="AC16PP_07010"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR6481413.1"
                     /translation="MGGDAIDLGTMLLGLLGGLAIFLFGLDQLTNMLKVTAGARMGDL
                     LAKMTTNRFKGVLAGGVTTAVIQSSSVTTVLVVGFVSAGLMTLQQSIGVIMGAEIGTT
                     ITAQIIAFKITEYALVAVVVGFVMQAFFDNDLVKRYGTMVFGLGLVFFGMFMMGEATR
                     PLRDYPPFVDLLAQMDNRVLTILLSAGFTALIQSSSA"
     gene            complement(665..>1929)
                     /locus_tag="AC16PP_07015"
     CDS             complement(665..>1929)
                     /locus_tag="AC16PP_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR6481414.1"
                     /translation="ATYDAALVAVDARTGEQVWRAVKADYRQGYTHTSGPIVGDGVVL
                     SGINGCERYKEDGCFVTGHDADTGRELWRTSTIALPGDVNDASWGGLEPSFRAGGDTW
                     IAGSYDPKLKLFFIGTSQAKPWVAASRGMTVDDAALYTNSTLAMDPRTGKIVWYFQHV
                     PGETIDMEVGFERVLVDVEGARLLFTVGKDGILWKLDRATGQYVAHVETIEQNIFEHV
                     DPETGRVRYREDIRSAGVGETFSACPGIYGGHNWQAMAYSPQARSLVIPLHRLCADMV
                     GRPVESKVGEGGFGGDSRSYEMPGSNGNLGRLVAVDAATLEERWVHEQRAMFMTGALT
                     TAGGLVFIGDLDRYVKAFDVADGRLLWQARLGAPTHGYPVTYRAGGKQYLAVPTGMGV
                     FRALTAVISPDIYQPANGQALYVFELPN"
BASE COUNT          375 a          657 c          570 g          327 t
ORIGIN      
        1 gccgacgagc tctgaatcag ggccgtaaat ccggcgctga ggagaatcgt gagaacccgg
       61 ttgtccatct gcgccagcag gtcgacgaaa ggggggtaat cccgcagcgg ccgcgtcgct
      121 tcgcccatca tgaacatgcc gaagaagacg aggcccaggc cgaagaccat cgtcccgtag
      181 cgcttgacca gatcgttgtc gaagaacgcc tgcatgacga accccaccac cacggcgacc
      241 agggcatact cggtgatctt gaaggcaatg atctgagcgg tgatcgtggt gccgatctcc
      301 gcgcccatga tgacgccaat ggactgctgc agcgtcatca ggcccgccga cacgaacccg
      361 acgacgagca ccgtggtgac cgacgacgat tggatcacgg ccgtggtcac accgccggcc
      421 agaaccccct tgaaccggtt cgtcgtcatc ttggccagca ggtcacccat gcgcgcaccg
      481 gcggtgacct tgagcatgtt ggtgagctga tcgagcccaa acagaaaaat cgccagcccg
      541 ccgagaaggc cgaggagcat ggttcccagg tcgatggcgt caccccccat gctcgctatc
      601 tcacccgact catcgtgacc gccccgtcac gttggccgcg agtgcccagg cgctgaaaga
      661 gcgctcagtt cggcaattcg aagacataca acgcctggcc gttggcaggc tggtagatgt
      721 cggggctgat gaccgcggtg agcgccctga aaacgcccat cccggtaggc accgccagat
      781 actgcttccc gcccgcacga taggtgacgg gatacccgtg tgtgggcgcg ccgagccgtg
      841 cctgccacag aaggcgcccg tcggcgacgt cgaatgcctt gacgtagcgg tccaggtcac
      901 cgatgaacac gagaccacct gccgtggtca gggccccggt catgaacatg gcccgctgct
      961 cgtgaaccca acgctcctcc agggtggcag catccaccgc aaccaggcgg cccaggttgc
     1021 cattgctgcc aggcatctcg taggagcgtg aatcaccccc gaacccgcct tctcccacct
     1081 tcgactccac ggggcgcccg accatgtcgg cacagagccg atgcagcgga atgacgaggc
     1141 ttcgcgcctg ggggctgtaa gccatcgcct gccagttgtg accgccgtag atacccggac
     1201 aggcgctgaa cgtctccccg acgccagcgg accggatgtc ctcgcgatag cgcacccgac
     1261 cggtctcggg atcgacgtgc tcgaagatgt tctgctcgat ggtttccaca tgagccacgt
     1321 actgccccgt cgcccgatcg agtttccaga gaatgccgtc cttgccgacg gtaaacagca
     1381 gccgtgcgcc ttcgacgtcc accagcaccc gctcgaaccc cacctccatg tcgatggttt
     1441 cacccggaac gtgctggaag taccagacga tcttcccggt tcgcggatcc atggccagcg
     1501 tggagttggt gtacaacgcc gcgtcgtcca ccgtcatgcc ccgactcgca gctacccagg
     1561 gcttggcctg ggacgttccg atgaaaaaga gtttcagctt cggatcgtag ctgccggcaa
     1621 tccaggtgtc gccgcctgct ctgaacgacg gctcgagtcc gccccagctg gcgtcgttga
     1681 cgtccccggg caatgcgatg gtggacgtcc gccacagctc acgacccgtg tccgcatcat
     1741 ggccggtaac aaaacatcca tcctccttgt aacgctcgca cccgttgatg ccgctgagaa
     1801 ccacgccatc gccgacgatg ggcccgctgg tatgtgtata gccttgtctg taatcggctt
     1861 tgaccgcgcg ccagacctgc tctccggtac gggcatccac agccacgaga gcggcgtcgt
     1921 aggtcgcca
//
LOCUS       DBNHKV010000221         3422 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1801581, whole genome shotgun sequence.
ACCESSION   DBNHKV010000221 DBNHKV010000000
VERSION     DBNHKV010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1801581"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="AC16PP_07020"
     CDS             <1..1262
                     /locus_tag="AC16PP_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR6481415.1"
                     /translation="DIVNNAKAAVAHMQFVSGPFVYFCSGGLINNSDQSALTPPPQPY
                     FLTANHCVSKGGEANSLQTYFHFEDCDGPQPAMVSGASILSSSRTSDYTLLALDDVPP
                     NTAVYLGWNSTPVASSSGLDLYRISHPSGAPQAYSKHSVSTSRPECRSWPRGPWIYSD
                     DDVGATEGGSSGSPVVNAAGQVVGQLSGACGYETSNVCSNRNATVDGAFAAYFNDVAQ
                     WLDPAPTSSGNNPPTASFRWSGDPDVMFTDTSTDLDENDSISSWAWNFGDGLTSTATS
                     PSHTYACNGTYNVTLTVEDTNGASDSASNAVTITAADVCENLTIGVKDPLAGSIDESG
                     RRPAVTFTINVATSDDGGDPNGAVVSGDWGGLVKGSGSCTVNGSSCEVIKGNIRGSGD
                     VTFTVTGIELSGYTFDGTEVTGTVQVP"
     gene            1331..1780
                     /locus_tag="AC16PP_07025"
     CDS             1331..1780
                     /locus_tag="AC16PP_07025"
                     /inference="COORDINATES: protein motif:HMM:NF025559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HIR6481416.1"
                     /translation="MSETRLTNLERTRIEMAYAVPLIRDLQRLLGAEVINDALSRRFD
                     DQIDATAAPGEKADFSRMEAGTATFAEGGALDYEIIGSDAEHFDLNITGCRYAEMMDE
                     LGARDIGHLLICNRDYPAAAKIGMALTRTQTRMQGAGYCDFRYRRRG"
     gene            complement(1781..2125)
                     /locus_tag="AC16PP_07030"
     CDS             complement(1781..2125)
                     /locus_tag="AC16PP_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HIR6481417.1"
                     /translation="MINIEHDYFTDEATPLAEIEAAGYHAVPLDFPAEENENHWHDFN
                     SLLYITEGELTVTYAETGETCTCGPGTKITGVPNVVHREKTEGYKALIGIPVPPAELT
                     QPINKPLPAPAG"
     gene            2289..3380
                     /locus_tag="AC16PP_07035"
     CDS             2289..3380
                     /locus_tag="AC16PP_07035"
                     /inference="COORDINATES: protein motif:HMM:NF014954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6481418.1"
                     /translation="MTNDELAVPETPADVTPAWLTRALGQRWPGIEVRSVVGEPLGEG
                     IGQISLLQRLTLEHAGAAEAPRHMVLKMHTTHADMLGVAVRFRMYEREANFYNRFADR
                     VPLRTPEVYHAATEGLERCVMLMEDMSSWHSPDQLAGPTLEEAELAVDSLATLTAAFW
                     NSPELSDHASWLPDFNVGYMSDTGNDYRQCLPEFLRRYETKLPPGSADAARTIADRYD
                     DLTRILSTGPQVLTHYDYRLENMFFRNDAAEAFCALDWQLVMRARPGWDIAYFMGTNL
                     PVSFLDEHGEALRDRYYQQLQSNGVQGYARADLEADLAIGTMAMTGIPIIGGANADLS
                     NPRNEALFTEVGSRAFGAVIGNDCLAYLP"
BASE COUNT          651 a         1143 c         1044 g          584 t
ORIGIN      
        1 ctgacatcgt caacaatgcc aaggcagctg tggctcacat gcagttcgtc tccggcccgt
       61 tcgtctactt ctgcagcggc gggctgatca acaacagcga tcagtctgcc ctcacaccgc
      121 cgcctcagcc ttactttctc accgccaatc actgcgtcag caagggcggc gaggcgaact
      181 ccctgcaaac gtattttcac ttcgaagatt gcgacgggcc tcaaccagcc atggtgtccg
      241 gggccagcat tctgagttcc agccgaacga gcgattacac gctgctggcg ctcgacgacg
      301 tgccgccgaa tacggccgtt tacctgggct ggaacagcac tccagtcgcc agttcttccg
      361 gcttggatct ttaccgtatc agccacccgt caggcgcgcc tcaggcgtac tcgaaacatt
      421 cagtcagcac cagccgtcct gaatgccgca gctggccacg cgggccgtgg atctacagcg
      481 acgacgacgt cggcgccacc gagggtggca gcagcggctc tccggtcgta aacgcggcag
      541 gccaagtcgt cggccaattg tccggcgctt gcggctacga aacctccaac gtctgcagca
      601 accgcaacgc taccgtggac ggtgcgttcg ccgcgtactt caacgacgtc gctcaatggc
      661 tcgatccggc accgacgagc agtggcaaca acccgcccac ggcgagcttc agatggtccg
      721 gcgatccgga cgtcatgttc accgacacaa gcaccgacct cgatgaaaac gacagcatct
      781 ccagctgggc ctggaatttc ggcgacggcc taaccagcac ggcaacgagc ccgagccaca
      841 cctacgcctg taacgggacg tacaacgtca cgctcacggt tgaagatacc aacggcgcaa
      901 gcgactccgc gtcgaatgcc gtcacgatca ctgccgccga cgtctgcgaa aatctgacga
      961 taggcgtaaa agaccccctg gcgggcagca ttgatgaaag cggccgccgg ccggcggtga
     1021 cctttacgat caatgtggcc accagcgatg atggcggcga cccgaacggc gctgtggtat
     1081 ccggtgattg gggcggactg gtcaaagggt ccggctcctg caccgtgaac ggttcgtcct
     1141 gcgaggtgat caagggcaac atccgcggca gcggtgacgt cacctttacc gtcacgggca
     1201 tcgagctctc cggatacacc ttcgacggca cagaagtcac cggcacggtt caggtcccct
     1261 gagcctcggg tagtcatgga gcaggcgcgg ccgcaaaccg cgccttccgc acagaatggg
     1321 ggaaccaccc atgagcgaga ccagactcac caacctggaa cgtacgcgca tcgagatggc
     1381 ctacgcggtg ccgctgatcc gcgatctgca gcggctgctc ggcgcggaag tgatcaacga
     1441 tgcgctttca cgccggttcg acgatcagat cgatgcgacg gccgcaccgg gcgagaaggc
     1501 cgacttctcc cggatggagg ccggtaccgc gacgttcgcc gagggcggcg cgctggatta
     1561 tgagatcatc ggctccgacg ccgagcattt cgacctgaac atcaccggct gccgatacgc
     1621 ggagatgatg gacgagctcg gtgcccggga catcggccac ctgctcatct gcaaccgcga
     1681 ctaccccgcc gccgcgaaga tcggcatggc ccttacccgc acccagacgc gcatgcaggg
     1741 cgccggctac tgcgacttcc gctaccggcg ccggggctga tcagccggcc ggtgcgggca
     1801 gcggcttgtt gatgggctgg gtaagctctg ccggcggcac gggaatgccg atcagcgctt
     1861 tgtagccttc cgtcttctcc cggtgcacga cgttcgggac accggtgatc ttggttccgg
     1921 gcccgcacgt gcaggtctcg ccggtctcgg cgtaagtgac cgtcagctcg ccctcggtga
     1981 tgtacagcaa cgagttgaaa tcgtgccagt ggttctcgtt ctcctcggcg gggaagtcca
     2041 ggggaacggc gtggtagccg gccgcctcga tctccgccag gggcgtcgcc tcgtcggtaa
     2101 agtagtcgtg ctcgatgttg atcatgttgc tcccctcatc cggatcgaac gtacacccac
     2161 attccggcct ctgtccactc ttgcatgatc gcaccggtcg atcaggtgtc acgcgccctg
     2221 tccgggttgt cccaccgttt actccgctct ccggccgatt taggctccga ggcagggagg
     2281 atacacgcat gaccaacgat gaattagcgg tacctgaaac acccgcagac gtcacgcccg
     2341 cctggctcac cagggcgctc ggccagcgct ggccgggcat cgaggtgcgc agcgtggtcg
     2401 gcgaacccct cggagagggc atcggtcaga tcagcctgct gcaacgcctg accctcgagc
     2461 acgccggagc tgcggaggcg ccccggcaca tggtgctcaa gatgcatacc acccacgccg
     2521 acatgctggg ggttgcggtg cgtttccgga tgtacgagcg ggaggcgaat ttctacaacc
     2581 gatttgcaga ccgggttccg cttcggactc cggaggtcta ccacgcggct acggaagggc
     2641 tcgagcgctg cgtcatgctg atggaggaca tgagcagctg gcactcaccc gaccagctgg
     2701 ctggccccac tctggaagag gccgagctgg ccgtggacag cctggcgacc ctgacggcag
     2761 ccttctggaa ctcgcccgag ctgtcggacc atgccagctg gctgccggat ttcaacgtgg
     2821 gttacatgag cgacacgggc aacgactacc gccagtgcct acccgagttt ctgcgccgct
     2881 acgaaacgaa gctgcccccc ggctccgccg acgcagcacg caccatagcc gaccggtatg
     2941 acgacctgac acgaatcctc agcacgggcc cccaggtgct gacgcactac gactaccgtc
     3001 tggaaaacat gttcttccgc aacgacgcgg cagaggcctt ctgcgccctg gactggcagc
     3061 tggtgatgcg ggcacggcct gggtgggaca tcgcctactt catgggcacg aacctgcccg
     3121 tgtcttttct ggacgagcac ggcgaggctt tacgcgaccg ctactaccaa cagctacaga
     3181 gcaacggggt ccaggggtat gcgcgcgccg atttagaggc cgacctcgca atcggcacca
     3241 tggccatgac gggcataccc atcatcggcg gcgccaacgc ggacctgtcc aacccgcgca
     3301 acgaagccct cttcaccgag gtcggcagcc gggctttcgg cgcggtgatc ggcaacgact
     3361 gcctggccta cctgccttga ttcaggaacc gatcaggcaa cgccgtcagg gtctgagctc
     3421 at
//
LOCUS       DBNHKV010000222         3912 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_315192, whole genome shotgun sequence.
ACCESSION   DBNHKV010000222 DBNHKV010000000
VERSION     DBNHKV010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_315192"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="AC16PP_07040"
     CDS             complement(<1..505)
                     /locus_tag="AC16PP_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011266417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR6481419.1"
                     /translation="MTDYKVADMSLADFGRREINLAEAEMPALMALRNRYADQQPLAG
                     AKVIGCIHMTVQTAVLIETLVALGAEVRWSSCNIFSTQDHAAAAMAAAGIPVFAWKGE
                     TEEEYWWCIEQTILKDGKPWDANLLLDDGGDLTEVIHEKYPEMLDSIHGVSEETTTGV
                     HRLYEMID"
     gene            complement(566..1522)
                     /locus_tag="AC16PP_07045"
     CDS             complement(566..1522)
                     /locus_tag="AC16PP_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HIR6481420.1"
                     /translation="MGQTAGQQANLDEVVEVAKAVGDHLRASILRVLSDGSYSVTELC
                     HLFQIPQPALSHHLKVLLTANLVAKRREGNNVYYRRCTEPDDGLRQRLFETVDGTPLG
                     AALAERAAEIHAERSQRSNNFFAENAHRFADQQALICEAQVYVPAIVELLDHAGVDSG
                     PIIEVGPGGGQLLATLAGSFDDLIGIDSSSTMLAQAAERLDGLPNVKLRQKDFTELPA
                     IRRYRAVIAAMVVHHLASPQRFFRHASRILRHGGLLIVAELDRHEQEWVTDACGDLWL
                     GFEPGELEQWAAQAGLAPVESQYLAQKNGFQIQIHGYARNGT"
     gene            1631..2791
                     /locus_tag="AC16PP_07050"
     CDS             1631..2791
                     /locus_tag="AC16PP_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR6481421.1"
                     /translation="MSWSSPDLVSKLPDVQTTIFTVMSALAREEGALNLSQGFPDFDG
                     PPELLERVQYYLTHGHNQYAPMTGVAPLREATAGKIADLYGCQLDPEQEVTVTSGATE
                     ALFCAIAAVVNAGDEVIVFDPAYDSYAPVVKLQGGVTRHVPLHPPDYRVDWDRVADNL
                     SDRTRLIIINTPHNPTGTVWGEDDIEALRAVIDGRDLFLLGDEVYEHIIFDGRQHASL
                     CRYPDLFARSFVVSSFGKTYHTTGWKIGYCAAPAPLSAEFRRIHQFVTFTSITPVQLG
                     LADFMTRHPEHHLGLGAFYEAKRDLFCSLLEGSRFGLTKSAGTYFQLLDYGDYSNEAD
                     TDLAVRLTREAKIASIPLSVFYADDPGHRMLRFCFAKDDATLRAGAEILLGL"
     gene            2861..3214
                     /locus_tag="AC16PP_07055"
     CDS             2861..3214
                     /locus_tag="AC16PP_07055"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6481422.1"
                     /translation="MKRPLLALLVCCAFGCSESSNETPASTAAAGDGPAPAAHPGEET
                     YNRFCFSCHAAGIAGAPRTGDARAWASRVAKGEDQLLKSTIEGIAPGMPAMGLCRQCS
                     EDDLRAAIDYMIPDA"
     gene            3404..>3912
                     /locus_tag="AC16PP_07060"
     CDS             3404..>3912
                     /locus_tag="AC16PP_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481423.1"
                     /translation="MTRYLPGDIVARRKGLVMHKGVVTGDGRIFHNTPFRGEHVCTEA
                     EFRAGKRLYVQRQEFEARQRAVEHAERHGGSGYNLFTNNCEHTVYRATSGVGHSPQLK
                     SWIAGLGLATVAFAVTRHPGAAAAGYALGRRLAGRSADRRRGSSPRGARPGVPARPPR
                     GRHGTCRTP"
BASE COUNT          669 a         1268 c         1253 g          722 t
ORIGIN      
        1 tatcgatcat ctcgtagagc cggtgcacgc cggtggtggt ctcctcgctg acgccatgga
       61 tggagtcgag catctcgggg tacttctcgt ggatcacctc ggtaaggtcg ccgccatcgt
      121 cgagaagcag gttggcgtcc cacggcttgc cgtccttgag aatcgtctgc tcgatgcacc
      181 accagtattc ctcctccgtc tcgcccttcc aggcaaaaac gggtatgccc gccgccgcca
      241 tggccgcggc ggcatggtcc tgggtggaga agatgttgca tgacgaccaa cggacttctg
      301 cgcccagggc gaccagggtc tcgatgagca ccgcagtctg caccgtcatg tgaatgcagc
      361 cgatgacctt cgcgccggcc agcggttgct ggtcggcgta gcggttgcga agcgccatga
      421 gcgccggcat ctcggcctcg gcgaggttga tctcgcgcct gccgaaatcg gccaggctca
      481 tgtcggccac tttgtagtca gtcatctgct ctttcctttt cgatgtgttg tctgaaatgg
      541 ttcgatgggt ttccgtatcc cggggctacg tgccgttgcg ggcgtagccg tggatctgta
      601 tctgaaagcc gtttttctgg gccaggtact gggactcgac cggcgccagg cccgcctggg
      661 ccgcccactg ctccagctcg ccgggctcga agcccagcca caggtcgccg cacgcgtcgg
      721 tgacccattc ctgctcgtgg cgatcgagct ccgccacgat gagcagaccg ccgtgccgca
      781 ggatccgcga agcgtggcgg aagaatctct ggggcgacgc caggtgatgc acgaccatgg
      841 ccgcgatcac ggcgcggtat cggcgaatcg ccggtagctc ggtgaaatcc ttctggcgca
      901 gcttgacgtt gggcagcccg tccagacgct cggccgcctg ggcgagcatg gtggaggagc
      961 tgtcgattcc gatcaggtcg tcgaagctcc ctgcaagcgt cgcaagcagc tgccccccac
     1021 cggggccgac ctcgatgatc ggcccggaat cgacccctgc atggtcgagc agctcgacga
     1081 tggctggaac gtatacctgc gcttcgcaga tgagcgcctg ctgatcggca aaacggtgtg
     1141 cgttttcggc aaagaagttg ttgctgcgct ggcttcgctc ggcgtgaatc tccgccgccc
     1201 gctctgccag ggcggcaccc aggggggtgc cgtcgacggt ttcgaaaagg cgctggcgca
     1261 atccgtcgtc gggttcggtg caccgccggt agtagacgtt gttgccctct cgacgtttcg
     1321 cgaccaggtt tgccgtcagc aggactttca ggtgatggct gagcgccggt tggggaatct
     1381 gaaagaggtg gcacagctcc gtgacgctgt aggatccgtc actgagcacc cgcaggatgc
     1441 tcgcccgcag gtgatcgccc acggctttgg cgacctcgac gacctcgtcg aggttggctt
     1501 gctggcctgc cgtctggccc atgggcggtg gtgcctctac gcaacgagag gcgatcctat
     1561 cagcgccttc gcacaatatc aaaaaatttt gatattgatg gcggaaagat cgccgtgtca
     1621 gaatgcggcg atgagttgga gctcgccgga tctcgtcagc aagctgccgg acgtgcagac
     1681 cacgatcttc acggtcatgt ccgcgctcgc cagggaggag ggcgcgctga acctttccca
     1741 gggtttcccc gatttcgatg gcccgcccga gctcctcgaa cgggttcagt attacctcac
     1801 ccacggtcac aaccagtacg cgccgatgac gggcgtggcg cccctgcgcg aagccacggc
     1861 gggcaagatc gcggatcttt acggctgcca gctggatccg gagcaggaag tgaccgtcac
     1921 ctccggcgcc accgaggcgc tcttctgcgc catagcggcg gtggtgaatg ccggtgacga
     1981 ggtgatcgtc ttcgatcccg cctacgacag ctacgcgccc gtggtgaagc tccagggcgg
     2041 cgtgacccgc cacgttccct tgcatccgcc ggactatcgg gtcgactggg atcgggtggc
     2101 ggacaacttg agcgatcgga ccaggctgat catcatcaac acgcctcaca accccaccgg
     2161 aacggtctgg ggcgaggacg acatcgaggc gttgcgcgcc gtgatcgacg gccgcgacct
     2221 gtttcttctc ggcgacgagg tctacgagca catcattttc gacgggcgcc agcacgcgag
     2281 cctgtgccgg tacccggatc ttttcgcccg cagcttcgtg gtctcgtcgt tcggcaagac
     2341 ctatcacacc acgggctgga aaatcggcta ctgcgcggcc cccgcgccgc tttccgccga
     2401 gttccgccgg attcatcagt tcgtcacctt cacctccatc acgcccgttc agctcggtct
     2461 cgccgatttc atgacccggc atccggagca ccatctcggg ctcggcgcgt tctacgaggc
     2521 caagcgcgat ttgttctgca gcctgctgga aggctcccgt ttcggcctga cgaagagtgc
     2581 cggcacctac ttccagctgc tcgactacgg cgactactcc aacgaggccg atacggatct
     2641 ggccgtgcgc ctcacccggg aggcgaagat cgcatccatt ccgttgtcgg tcttctatgc
     2701 tgacgatccc ggccaccgca tgctgcgttt ctgctttgcc aaggatgacg ctaccttgcg
     2761 agccggtgcg gagatactct taggcctgtg aagccgacgc cgccggatga gcgcgcgcga
     2821 acgggatgaa tccgatgacc gacatgagga gaaaaacggg gtgaaacgtc cacttctggc
     2881 attgctggtc tgctgcgcat ttggctgctc ggaatcatcc aacgagacgc ccgcgtcgac
     2941 ggcggccgca ggtgacgggc cggccccagc cgcacatccg ggagaagaga cctacaaccg
     3001 cttctgcttt tcctgtcacg ccgccgggat tgccggagcg cctcgaaccg gcgacgcgcg
     3061 tgcctgggct tcgcgggtgg caaaggggga ggaccagctg ctcaagtcga ccatcgaggg
     3121 catagcgccc gggatgcccg ccatggggct gtgcaggcaa tgctccgagg acgacctgcg
     3181 cgccgccatc gactacatga tcccggatgc ttagccgccc tcgccagatg ttggcgcaaa
     3241 cgccacccgg ccacagcttg atcgacaaaa aaggcaatca tatcaatggg ttgaatagtg
     3301 gaacggatgt tgctaaacga atgccgagac gaattgtcga atcatgagaa cgacccgggt
     3361 ggcaaggaga ttgccccccg ccaactgaca ggaccttaat cacatgaccc gctatcttcc
     3421 tggagacatc gttgcccgcc gcaagggtct ggtgatgcac aagggtgtgg tgaccgggga
     3481 cggccggatc ttccacaaca ccccgttccg gggcgagcac gtctgcaccg aggcggaatt
     3541 tcgcgccggc aaacggctgt acgtgcagcg ccaggagttc gaggcgcgcc agcgcgcggt
     3601 cgagcacgcc gagcgccatg gcggtagcgg ctacaacctg ttcaccaaca actgcgagca
     3661 cacggtgtac cgggccactt ccggcgtcgg tcacagcccc cagctgaagt cgtggatcgc
     3721 cgggctgggg cttgcgaccg tggcctttgc ggtcacccgc caccccggcg cagcggccgc
     3781 cggctatgcg ctgggccgcc ggctggccgg ccggtcggcc gaccggcgac gcggatctag
     3841 tcctcggggc gctcgtccgg gcgtcccagc ccgcccgccc cgggggcggc acggtacgtg
     3901 ccgaaccccg ga
//
LOCUS       DBNHKV010000223         8987 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1590447, whole genome shotgun sequence.
ACCESSION   DBNHKV010000223 DBNHKV010000000
VERSION     DBNHKV010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8987
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1590447"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="AC16PP_07065"
     CDS             <1..330
                     /locus_tag="AC16PP_07065"
                     /inference="COORDINATES: protein motif:HMM:NF045268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HIR6481424.1"
                     /translation="RDDRRDPGCRAVTGEREAAPLIAEMPAPAALPRKSGEMVFHDEW
                     ERRAFAMAVSLAEQGAFEWSEFQQKLIAAISDAERDDPQQPSRGYYESWLVALEALLQ
                     EKELLPG"
     gene            520..1038
                     /locus_tag="AC16PP_07070"
     CDS             520..1038
                     /locus_tag="AC16PP_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6481425.1"
                     /translation="MHGIQVEGLMMPSGFRSHRAQVAVCTDLADLRAFISDTSRFHEW
                     IPDLAEVRLLEQSDDGVVYYMRSKAPWPVKDRDMVYRLFGVPESGDAASVALAIEGLP
                     GRLPEVDGAYRMHDVSGQWRIWERGDRLDVQQQIYLNPGDVPRMFANRSMSASLARTL
                     GNLASRYPCPKS"
     gene            complement(1062..2168)
                     /locus_tag="AC16PP_07075"
     CDS             complement(1062..2168)
                     /locus_tag="AC16PP_07075"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6481426.1"
                     /translation="MTDEPIAPVTVYGSSISYFTGKLENYLRIKGIPYELKPLGPGSV
                     RKLIKSRTGSSQMPAVRLEDGRWLTDTTPVIGWFEREYPEPAVIPHDPLQRFFSLLLE
                     DYADEWLWRPAMHYRWFYDVGARFASAHLAQELTRGVYLPMFVKRRAIARRQRDGYTR
                     GDGISTDQVPGVEAIYSRTLGQLETIFRSRPFLLGASPSLADVGFSGPFFRHFGLDPV
                     PAEIMRRDAPAVYEWIARLWNAGPVDSSMAIPAGIPDDWAPILDEIGTHYLPYLCANV
                     DAVAAGLRRFDAEVGGVRYHRARWSPYRVWCLKKLRETFAGLTEDEQAEARTLLERHG
                     CWEPMWRHASLPLKDGFCERLPFHADAKMIDVYQ"
     gene            complement(2158..2892)
                     /locus_tag="AC16PP_07080"
     CDS             complement(2158..2892)
                     /locus_tag="AC16PP_07080"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR6481427.1"
                     /translation="MQMWRFLAPSALGFTSRNMWHALIDRATGAPPRALRAMEHVAAH
                     AERHNPQDVLRKLDEFATSVRWMMSIGPEKDKVIAEARAALDRDPRVLELGAYAGYSS
                     IYMADVLGPGTNITSVEVNADNVRAARGNIAHAGLDGRVEVVHGSSSDVIPTLEGSFD
                     LVFLDHWKDLYLQDLQLIETQGLIAPGSVVVADNVGELFGADAYLDYVRHCGRYDSAN
                     RPATIEYTSLPDAVEISVYRGQGDDG"
     gene            3024..3362
                     /locus_tag="AC16PP_07085"
     CDS             3024..3362
                     /locus_tag="AC16PP_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481428.1"
                     /translation="MDEIRSYHYDPEKFEPYRKWALEEAIPFLKDNLNIVGFWLDNGK
                     APEVGGTSPMDMPLGSANVTWVIRWDSEEDREAGHARIFGGQEWQDIWSRHPDANGYL
                     QMEARFADPV"
     gene            complement(3368..5407)
                     /locus_tag="AC16PP_07090"
     CDS             complement(3368..5407)
                     /locus_tag="AC16PP_07090"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HIR6481429.1"
                     /translation="MNDRSRPRSPIAGPALFVTVLAAASAVAEVEWRSYGNDHTEQRF
                     SPIDQVNRSNVGELGLDWALSVPDAVSLNSTPLMIDGVLYFSADRAIVHAVDARSGKL
                     IWSYDPQSWKHAPRGIGVSFNTNRGIAHYEGSIFVGTADGRLVSLDARTGRVKWATRT
                     FGREERKAITGAPRAFDGKVFIGNGGAEFGTRGFVSAYDAADGELLWRFYTVPGDPSL
                     GFENDAMAKAAKTWHGDWWKVFGGGTVWNGLTYDPELDLLYVGVGNGDPWDHDLRSQG
                     KGDNLYLCSIVALKADTGEYVWHYQVNPGEQWDYKATMDMILADLPMGGTTRKVLMQA
                     PSNGFFYVLDRATGELLGAEKYERATWAERIDLKTGRPVETPGIRMKPGEETLIYPGP
                     YGAHNWQAMSFNPGTGLAYIPTIRYPAIYSKNTAYEQREGFFVIGMNTVYATPEPGDG
                     KGGLVAWDPVKGEARWRIQYDSIWNGGTLTTSGGIVFQGTADGNFLAVDASSGEELWR
                     FDVQRGVTAAPISYTLDGRQHIVLPVGWGGQSAFGPPAFRAHRIPYKGEDVRLLSFSL
                     DGKASLPPPPARPTEIQTANTGDEPIDEALAVKGFNIYHQASCAVCHGGEAISVGAAA
                     PDLRESPYMANYPAFRAVVVEGALLPRSMPMFDDLTETEVRAVWEYVRQQTRLVD"
     gene            complement(5442..5867)
                     /locus_tag="AC16PP_07095"
     CDS             complement(5442..5867)
                     /locus_tag="AC16PP_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HIR6481430.1"
                     /translation="MPPSTPYQILGQEGINQLVDAFYDAMDELTEAAHVRAMHKENLE
                     EVRQKLKDYLIGWMGGPPIYQMKTGTVCLTDPHAPYAIGPKERDQWLLCMDTALERIG
                     ASDELKEMLKIPMFRVADTVRNRDSSEPKPRDPNIIAVG"
     gene            complement(5923..6822)
                     /locus_tag="AC16PP_07100"
     CDS             complement(5923..6822)
                     /locus_tag="AC16PP_07100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HIR6481431.1"
                     /translation="MKIVIAGGTGLIGRALSTRLAGAGHEVVVLSRQRQAPTGSTVAS
                     WDGRSPGAWQDVLRGAGALINLCGASIGEARWTPTRKRELADSRTLPSVALAQACAAV
                     EDPPKVLLQASGVGFYGTSETTTFDESSPPGEDFLARLAQEWESAGADLLPPETRRVV
                     MRMGVVLSREGGVLEQLLLPFRLFAGGPIGSGRQFLSWIHIDDLTAAMEFLIGTSQLS
                     GPVNLVAPEVPRNTQVARAIAEALHRPSWFPIPAWAMRLALGEMSSLVLEGQRVIPRE
                     LQAAGFRFSYPNLRSALAQILAN"
     gene            complement(6864..7139)
                     /locus_tag="AC16PP_07105"
     CDS             complement(6864..7139)
                     /locus_tag="AC16PP_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481432.1"
                     /translation="MHTTVTVRSGTTTATGNTGEDTTEGTGTDITSATGIDMRDGIAT
                     TATAGTIGALTTGTVTATTANQAATASDVEVTATDSSAPIHFGGHNR"
     gene            complement(7366..8478)
                     /locus_tag="AC16PP_07110"
     CDS             complement(7366..8478)
                     /locus_tag="AC16PP_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR6481433.1"
                     /translation="MTHVPTRFTTSLRTAIVLLAALMGSQCSASDASPDHPEAVILVY
                     HHVALGTPASTSVTPDRFAEHLGLLEHGGYAVIALQRVIAALERGEPLPPRSVALTFD
                     DAYRSVYTQALPALEARGWPFTVFVSTRAVDEGHANTMTWDELRDLEARGGAVGNHSR
                     SHDHLVRPRPGEKAEDWRARVIDDVLAAQSRLEEELERPLSAFAYPYGEFDLEIRNLI
                     RTLGFLAVGQQSGPVGTGTDPWAIPRFPVATGFDSPDRLREKLQTVALPVNVVAPSSN
                     TLTADAGPPELELVMSSGSFDPSRLVCYVPGQPPAKIRWVDRSAGRLTVTAREPLDPG
                     RSKYTCTAPSSRAAGVYHWYSHLWMKPNPDGSWPRD"
     gene            complement(8488..>8987)
                     /locus_tag="AC16PP_07115"
     CDS             complement(8488..>8987)
                     /locus_tag="AC16PP_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6481434.1"
                     /translation="ASGSFLNSRASYTSTRGGREQPALKRTTMAAMPQEASAMFTVLG
                     LDHVVIRASDPGKLIGFYQEVLGCRLERELPELGLFQLRAGRSLIDVLARPDAAEPCE
                     PNMDHFCLRVEPFDVQSLARHFEGLDIPARIATRVYGAEGYGPALYIQDPEGNTVELK
                     GPAGG"
BASE COUNT         1480 a         2865 c         3013 g         1629 t
ORIGIN      
        1 cgcgatgatc ggcgtgaccc gggttgccgc gccgtgaccg gtgagcggga agccgcgccc
       61 ctcatcgcgg agatgccggc accggcggcg ctgccgcgca agagcgggga aatggtcttt
      121 cacgatgagt gggaacgccg tgccttcgcc atggcggtga gcctggccga gcagggcgct
      181 ttcgagtggt cggagtttca gcagaagctc atcgcggcga tatcggatgc ggagcgggat
      241 gaccctcagc agcccagccg gggctactac gaaagctggc tagtggccct ggaggctctt
      301 ctgcaggaga aagagctgct tccaggctag ctgctgcgcg ccgtggcgcg ccctcggtca
      361 tcggtgagct cgccgccgga tgcggtgagt cccctgcggc tgataccatc ggcgccgtcc
      421 tttcatgtgg ctgtggtttt gcgcctgtat ctgatcgtcc ttttgctctg ggccgcgtcc
      481 gcttcagccg cgcaggccga ttgggagcct gtacggaagg tgcacggtat ccaggtcgag
      541 ggtctgatga tgccgtccgg tttccggtcc catcgtgctc aggttgccgt ttgcaccgat
      601 cttgcggatc tgcgtgcgtt catttccgac acctcgcgat ttcacgagtg gattcccgat
      661 cttgccgagg tgcgcctgct cgagcagtcc gacgacgggg tcgtctacta catgaggagc
      721 aaggcgccct ggccggtgaa agatcgagac atggtgtacc ggctcttcgg cgtgcccgag
      781 tccggggacg ctgccagcgt ggcgctcgcc atcgagggcc tgcccgggcg gctgcccgag
      841 gtggatggcg cctatcgaat gcacgacgtt tccggtcaat ggcgcatatg ggaacggggt
      901 gaccggctcg acgtgcagca gcagatctac ctcaaccccg gggacgtccc gcggatgttc
      961 gccaaccgca gcatgtcggc cagcctggcc aggacgctcg gcaatctggc ctcgcgatac
     1021 ccctgcccga agagctgaag gatagcagcg acgtcgcgcg gctattgata cacgtcgatc
     1081 atcttggcat cggcgtggaa gggcagccgc tcgcagaaac catccttcag cggcagcgag
     1141 gcgtgccgcc acatcggctc ccagcagccg tgccgctcga gcagggttct ggcttcggcc
     1201 tgctcatcct cggtgaggcc cgcgaacgtc tcgcgcagct tcttcaggca ccagacccga
     1261 tagggagacc agcgcgcacg gtggtagcgc acgccgccga cctccgcgtc gaagcgcctc
     1321 aggcccgctg caaccgcgtc cacgttggcg cagaggtagg gcaggtagtg cgtgccgatc
     1381 tcgtccagga tgggcgccca gtcgtcggga ataccggccg gaattgccat gctggagtcc
     1441 acggggccgg cgttccacag ccgggcgatc cactcgtaga ccgcgggcgc gtctctgcgc
     1501 atgatctcgg cagggaccgg atcgagcccg aagtgacgga agaaggggcc ggagaagccg
     1561 acgtccgcca ggctggggct ggcacccagc aggaagggcc gggatcggaa gatcgtttcg
     1621 agctgtccca gggtccgcga gtagatggcc tccacacccg ggacctggtc ggtgctgatg
     1681 ccgtctcccc tggtgtagcc gtcgcgttga cgccgggcga tggcgcgacg cttgacgaac
     1741 atgggcaggt atacaccccg cgtgagctcc tgggccagat gggcgctggc gaagcgagcg
     1801 ccaacgtcgt agaaccagcg gtagtgcatg gccggtcgcc aaagccactc gtcggcgtaa
     1861 tcttccagca gcaggctgaa gaaccgctgc agcggatcgt gcggaatcac cgccggctcg
     1921 ggatactccc gctcgaacca cccgatgacg ggcgtggtat cggtcagcca gcgcccgtcc
     1981 tcaagccgta ccgcgggcat ctgcgaggat ccggttctgc ttttgatgag cttgcgaaca
     2041 gagccggggc ccaggggttt gagctcgtag ggtatgccct tgatgcgaag gtagttttcc
     2101 agcttccccg tgaagtacga gatgctcgac ccgtaaacgg tgaccggcgc gatgggctca
     2161 tccgtcatcg ccttgccccc ggtataccga gatctctacg gcatccggca acgacgtgta
     2221 ctcgatggtg gccgggcggt tggccgaatc gtagcgcccg cagtgacgga cgtagtccag
     2281 gtaagcgtcc gcgccgaaca gctcgcccac gttgtctgct accacgacgg agcccggcgc
     2341 gatcaaccct tgggtttcga tcagctgcag atcctggagg tagaggtcct tccaatgatc
     2401 cagaaacacg agatcgaagc tcccctcgag tgtcgggatc acgtcgctcg agctgccgtg
     2461 caccacctct acgcgcccat cgagacccgc gtgcgctatg ttgccccgtg cggcccgcac
     2521 gttgtcggca ttcacctcta cggaggtgat gttcgtgccc ggcccgagaa cgtctgccat
     2581 gtagatcgac gagtaaccgg cgtaggcgcc cagctccagg acgcgcggat cgcggtccag
     2641 cgccgctctg gcctcggcaa tgaccttgtc cttctccggg ccgatgctca tcatccagcg
     2701 caccgacgtt gcgaactcgt cgagcttgcg gagcacgtcc tgggggttgt gccgttcggc
     2761 atgcgctgcg acgtgctcca tggctcgaag cgccctgggc ggcgcgccgg tggcgcggtc
     2821 gatcagggcg tgccacatgt tccggctggt gaatcccagc gccgagggtg cgagaaagcg
     2881 ccacatctgc atgatgggac ctccttgggt tgatgccgat ttcgctggca cgagatcgta
     2941 ccgcaaatcg cccgactgct tttgccatca gccgttagcg gctaaggttg cgggttaatt
     3001 gccaaccgac ccggtgaccc gccatggacg agatccgcag ctatcactac gaccctgaga
     3061 agttcgagcc ctacaggaag tgggcgctcg aggaagccat accgtttctg aaagacaatc
     3121 tgaacatcgt cggcttctgg ctcgacaacg gcaaagcgcc ggaggtcggc ggcacgtcgc
     3181 ccatggacat gccgctgggc agcgcgaacg tcacctgggt catccgctgg gacagcgaag
     3241 aggaccgcga agcgggtcat gccaggatat tcggcggcca ggaatggcag gacatctggt
     3301 cccgtcaccc cgacgccaac ggctacctgc agatggaggc gcgtttcgcc gatcccgtct
     3361 gagggggtca gtccaccagg cgggtctgct gccggacgta ctcccacacc gctcgaacct
     3421 cggtttcggt gaggtcgtcg aacatgggca tgctgcgcgg cagcagcgcg ccttcgacca
     3481 ccacggcgcg aaaggcgggg taattcgcca tatacggcga ctcccgcaga tcgggggcgg
     3541 cggcaccgac ggatatggcc tcgccgccat gacaaaccgc gcagctggcc tggtgataga
     3601 tgttgaatcc ctttactgcc agcgcctcgt caatgggctc gtcgcccgtg ttcgccgttt
     3661 gaatctcggt gggccgggct ggaggaggcg gcaggctggc tttgccgtcg aggctgaagc
     3721 tcagcaggcg cacgtcctcg cccttgtagg ggatacggtg tgcccggaac gccggcggcc
     3781 cgaacgccga ctgcccgccc cagcccaccg gcagcacgat gtgctgcctg ccgtccagcg
     3841 tgtagctgat gggcgccgcg gtgacgccgc gctgcacgtc gaatcgccac agctcctcac
     3901 cgctggaagc gtccaccgcg aggaagtttc cgtctgcggt cccctggaac acgatgccgc
     3961 cggacgtggt cagcgtgccg ccgttccaga tcgaatcgta ctgaatgcgc cagcgtgcct
     4021 cgcccttgac gggatcccag gctaccagcc cgcctttgcc gtcgccgggc tcgggcgtcg
     4081 cgtagactgt gttcatcccg atcacgaaaa aaccctcgcg ctgctcgtag gccgtgtttt
     4141 tcgagtagat ggccggatag cggatggtgg gaatgtaggc cagccccgtg ccgggattga
     4201 aagacatggc ctgccagttg tgcgcgccgt aggggcccgg gtagatcaag gtctcctcgc
     4261 cgggtttcat ccggatgccg ggcgtctcca cggggcgccc ggttttgagg tcgatgcgct
     4321 ccgcccaggt ggcacgttcg tatttctccg cgcccagcag ctctccggtg gcccggtcga
     4381 gcacgtagaa aaacccgttt gacggcgcct gcatcagcac cttccgggtg gtgccgccca
     4441 tgggtaggtc cgccaggatc atgtccatgg tggctttgta gtcccactgc tcgccgggat
     4501 tgacctggta gtgccagacg tactctccgg tgtctgcctt cagcgcgacg atggagcaga
     4561 ggtacagatt gtcgcctttg ccctggctcc tgagatcgtg atcccacgga tcgccgttgc
     4621 ccacgcccac gtacaacaga tccagctcgg gatcgtaggt cagcccgttc catacggtcc
     4681 cgccgccgaa caccttccac cagtcgccgt gccacgtctt ggcggctttc gccatggcgt
     4741 cgttttcaaa gcccagggac gggtctcccg gcaccgtgta aaaacgccag agcagctcgc
     4801 catctgccgc gtcgtaagcg gagacgaatc ctcgggttcc gaattctgct ccgccgttgc
     4861 cgatgaagac tttcccgtcg aaggcacgcg gcgcgccggt tatcgctttg cgctcctcgc
     4921 gcccgaacgt tcgcgtggcc catttcaccc tgccggtcct ggcatccagc gagacgagcc
     4981 tgccatcggc ggtgccgacg aagatgctgc cctcgtagtg ggcgatgccg cggttggtgt
     5041 tgaagctcac cccgattccc cggggcgcgt gcttccagct ctgggggtcg taggaccaga
     5101 tgagcttgcc gctccttgcg tccacggcgt gcacgatggc tcggtcggca gaaaagtaga
     5161 gcacaccgtc gatcattagg ggcgtgctgt tgagcgacac ggcgtccggc acgctcagcg
     5221 cccagtcgag tccgagttcc cccacgttgc tccggttgac ctggtcgatg gggctgaagc
     5281 gttgttcggt gtggtcgttg ccgtagctcc tccactcgac ttccgccaca gccgaagcgg
     5341 ctgccagcac agtgacgaac agagcgggcc cggcaatggg cgagcgcggt ctcgatcggt
     5401 cgttcatgga cctgcgatgg ggtttcgttt ccaggatccg ttcagcccac ggcgatgatg
     5461 ttcggatcgc gcggtttcgg ctccgacgaa tcccggttgc gcacggtgtc tgcgacccgg
     5521 aacatcggga tcttgagcat ttccttcagc tcgtccgatg cgccgatacg ctcgagggcc
     5581 gtatccatgc acagcagcca ctgatcccgc tccttgggcc cgatggcgta gggggcgtga
     5641 gggtctgtca ggcagacggt gccggtcttc atctggtaga tcggcggccc gcccatccag
     5701 ccgatgaggt aatccttcag cttctggcgg acctcttcga ggttttcctt gtgcatggcg
     5761 cgtacgtgag ccgcctcggt gagctcgtcc atggcgtcgt agaaagcgtc cacgagctgg
     5821 ttgatacctt cctggccgag aatctgataa ggcgtcgatg gcggcatggc ggtctcgcgt
     5881 cggttgctga cgaatcagca tacccaaggg gattacgggt aatcaattgg ccagaatctg
     5941 tgccagcgcc gatcgcaggt tggggtagga aaagcgaaac ccggctgcct gcagctcgcg
     6001 ggggattacc cgctgaccct ccaggacgag actggacatc tcgcccaggg ccaggcgcat
     6061 cgcccacgcc ggtatgggaa accagctcgg ccggtgcaga gcttccgcga tggcacgggc
     6121 gacctgagtg ttccgcggta cttcgggcgc gaccaggttg acggggccgg atagttggct
     6181 ggtgccgatg agaaactcca tggccgcggt cagatcgtcg atgtggatcc aggacaagaa
     6241 ttgtcgaccg gagcctatgg gtccgcccgc gaagagccga aacggcagga gcagttgctc
     6301 caggacgcct ccttcacggg acaacacgac gcccatgcgc atgacgacgc gccgcgtctc
     6361 agggggcagc aaatcggcgc ccgcgctctc ccattcctgt gccagccgag cgagaaagtc
     6421 ctcgccgggc gggctcgatt cgtcgaaggt cgtggtttcg gaggtcccat agaaaccgac
     6481 gcccgatgcc tgcagcagca ccttgggagg atcctcgacg gcggcgcatg cctgggcgag
     6541 agcaactgac ggcaaggtgc gcgagtcagc cagctcccgc ttgcgagtcg gcgtccagcg
     6601 ggcctccccg atgctcgcgc cgcacaggtt gatcagggcg ccggcgccgc gcagcacgtc
     6661 ttgccacgcc ccgggggaac ggccgtccca tgacgccacg gtgctgcccg tcggcgcctg
     6721 gcgctgtcgc gacagcacca ctacctcgtg accggcgccg gccagtcggg tagacagtgc
     6781 ccggccgatg aggccggtgc cgccggcgat tacgatcttc accagccggt taccaacgcg
     6841 ttccgttcaa tgagttgccg accttagcgg ttgtgcccgc cgaagtgaat cggcgcggag
     6901 ctatcagtgg ccgtgacctc gacgtcgctc gccgtggcgg cctggttcgc ggtcgtagcg
     6961 gtgaccgtgc cggtcgtgag cgcgccaatg gtgccagcgg tggcggtcgt agcgatgcca
     7021 tcgcgcatgt cgattccagt cgcgctggta atgtccgtac cggtaccctc cgtggtgtcc
     7081 tcgccagtgt tgccggtagc ggtggtggtc ccgctgcgca cggtcactgt agtgtgcata
     7141 cgtcagggct acgtcggcgg ccgtcagcca gggttcgtgg cgttcgtgcg cggacgcgac
     7201 gaagggaatc gtgaaaccga taactgcgac cgtcacgccg atcagtcttg tgcggggttt
     7261 gatcatctct gcctccgagt tccgattggg caggatcaaa ggtattcccg ggccattgaa
     7321 cgcaggctga actgaacctg acgccttcct gaacgacggc cgggatcagt cccgcggcca
     7381 ggacccgtcc ggattgggct tcatccagag gtggctgtac cagtggtaca cgcccgcggc
     7441 gcggctggaa ggcgccgtgc acgtatattt cgatcggccc gggtcgaggg gctcgcgggc
     7501 ggtgaccgtg agccgtcctg cggatcggtc cacccagcgg atcttggcag gcggctggcc
     7561 cgggacgtag cagactagcc ggctcggatc gaagctgccg gagctcatta ccagctcgag
     7621 ctcgggaggc ccggcgtcgg ctgtaagcgt gttcgagctc ggagcaacga cgttcacagg
     7681 cagggcaacg gtttggagct tctccctgag ccgatccggc gagtcgaagc cggtggccac
     7741 cgggaagcgg ggtattgccc acggatcggt cccggtgccc acgggtcccg actgctgccc
     7801 gaccgcgaga aaaccgagcg ttcggattag attcctgatc tccagatcga actcgccgta
     7861 gggataggcg aacgccgaca ggggtctttc gagctcctcc tcgagtcggc tttgggcagc
     7921 aaggacgtcg tcgatcaccc gcgcgcgcca gtcttccgcc ttctcccccg gccgcgggcg
     7981 aaccagatga tcgtgggagc gggagtggtt gcccaccgcc ccgccccggg cttcgaggtc
     8041 cctcagctca tcccatgtca tggtgtttgc atgaccctcg tcgactgccc gggtgctgac
     8101 gaaaacggtg aacggccatc ctcgagcttc cagagcgggg agggcctggg tatagaccga
     8161 ccggtaggcg tcatcgaagg tgagcgccac gctgcgcggt gggagcggct cgccccgctc
     8221 cagggcggcg atcacccgtt gcagcgcgat gacggcatac cccccatgct cgagcagccc
     8281 gaggtgctct gcaaagcgat cgggcgttac gctggtgctg gcgggcgtgc ccagcgcgac
     8341 gtggtggtag acgaggatta ctgcttcggg gtgatcgggg gaagcgtcgg aagccgagca
     8401 ctgagacccc atgagcgcgg ccagcaggac gattgccgtg cgcaggctcg tcgtgaagcg
     8461 ggtgggaacg tgcgtcacgc cagggcctca gccgcccgcc gggcctttga gctcgacggt
     8521 attgccctcc ggatcctgta tgtagagcgc cggaccgtaa ccttcggcgc cgtacacccg
     8581 ggtagcgatc cgggcaggga tgtccaggcc ctcgaaatgc cgggccagcg actgcacgtc
     8641 gaacggctcg acgcgcaggc agaagtgatc catgttgggc tcgcagggct cggctgcgtc
     8701 gggtcgggcg agcacgtcga tcagcgaacg tccggcccgc agctgaaaaa ggcccaactc
     8761 gggcagctca cgctcgagcc tgcatcccag cacttcctgg taaaagccga tcagcttgcc
     8821 gggatcgctg gcgcggatca ccacgtggtc gaggccgagg acggtgaaca tggctgatgc
     8881 ttcctggggc atggctgcca ttgtagtgcg cttgagggcg ggttgctcac gtcctccccg
     8941 tgtgctggtg tatgatgccc ggctgttcag gaacgaccca gacgctt
//
LOCUS       DBNHKV010000224         4288 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1306688, whole genome shotgun sequence.
ACCESSION   DBNHKV010000224 DBNHKV010000000
VERSION     DBNHKV010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1306688"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="AC16PP_07120"
     CDS             <1..354
                     /locus_tag="AC16PP_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481435.1"
                     /translation="VVLMRNAWIASDAATVEEEWLGAMVDFHNLYSDARAAGESGDTV
                     LERLLAGEEIGFEEYVEGRSIAGSPSMCIAEIERWQAAIQPDEYSLIFGGSDDAARLE
                     DAVTLFAREVMPAFS"
     gene            369..788
                     /locus_tag="AC16PP_07125"
     CDS             369..788
                     /locus_tag="AC16PP_07125"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6481436.1"
                     /translation="MAASLEDNPMPVNLIDYMPFARVLGLEVTHSDKERVTASIEVSE
                     DLCTTGKIMHGGAVMAVADTLGAIGAYLNLPDGAKATTTIESKTNFLGAARLGTRVLA
                     ETTPVHVGGRTSVWQTRLTGEDGKPVALVTQTQLVLS"
     gene            880..2802
                     /locus_tag="AC16PP_07130"
     CDS             880..2802
                     /locus_tag="AC16PP_07130"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HIR6481437.1"
                     /translation="MADIFISYARSTRRQAEAIGEALVELGYSIWRDDALPAHRAYAD
                     EIQKQLNIAKAVVVVWSADAIKSDWVRSEADRARNDRKLVQLTVDGAALPMPFDQIQC
                     ANLAGWRGEGRAAGWATVLESIRALTDTAGKESPVSAGTQSQQQPEPILAVLPFDNLS
                     TDPEMQFFSDGVSEEIIQRLSRGASLKVIGRTSSFQFRGERKAEAASALGCSHILDGS
                     VRRGAGRMRLSAHLVEASSGTTLWSERYDRSLEDVFAVQDDVSENIAGALHHTFQSFS
                     TPAVDPGAYDLYLRASPESYAPEEMRTNIGLLEVATQRAPDFPEAWGRLAYLRAFLRF
                     YEPFPGRGASAAIIARESAHALQADPENGDALAARAFVVAPFGNFIELDTAVERLRSA
                     PGDFDARKFAGWLLRNFGRVRESLAEDERAYLGNALDPMCANTLALARLAAGRVAEAL
                     PVLENLVERVPDMVFPFANLLRAQALLENWEALDELLELNADRPLREFQDTLDFIRTK
                     RNPSAENIAVWKSQFEAHIARTGCIDISRVVYAAHLGLVDEAYQATEQAHLGPTGTNE
                     DVMGPDGYRTSLLFQAAMPELRNDPRFVPLCARLGLVEFWTSTGKWPDCADEVPYDFR
                     AACEDARGIPKLEFGF"
     gene            complement(2847..4187)
                     /locus_tag="AC16PP_07135"
     CDS             complement(2847..4187)
                     /locus_tag="AC16PP_07135"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6481438.1"
                     /translation="MVTSRQEALVQRSQAVSGSVSRIFPADVTITGERYTSREFMELE
                     LDRLWPRVWNIGAWAREIPVAGDFVTHAIGRESILMVRQADGSVRAFHNVCPHRGNRL
                     VYSDRGSVERFTCSYHGWQFDQAGTVVHVQDEEDFPRGNPCGKLTLAEIPCEVWAGFV
                     WYNMDAECASLAEFLGEVKDYVDLHDLSAARRIYYKVCEVGFNWKLLHDNFCESYHLP
                     ATHPQISDYYDDDYRNTDFELYETGHNLMKMKGSLPSLRYDEPFAINETLAADMQNWG
                     LDPAAFEGRAHAVREALQAQRRKLGPARGYRYFERLPASWLTDAYHFNLFPGTSLTVV
                     ADGAQMQRCEPHPTDPTRSIYEHWFLALKGADSDLVPGPEGMVPWEEAERRVVEYGQE
                     TLSEVADQDLERASGQQLGVQSRGFAGAYLSGQENRVQQFHNFIDRYLRDNIGS"
BASE COUNT          718 a         1456 c         1387 g          727 t
ORIGIN      
        1 gtcgtcctca tgcggaatgc atggatcgcg tcggacgccg ccacggtgga ggaagagtgg
       61 ctcggagcga tggtggactt ccacaacctg tactccgacg cgcgggccgc aggcgagtcg
      121 ggggacaccg tgctcgagcg gctgctcgcc ggcgaggaga tcggattcga ggagtacgtc
      181 gagggccggt cgatcgccgg cagcccctcg atgtgcatcg ccgagatcga gcgctggcag
      241 gcggccatcc agcccgacga gtactcgctc atcttcggcg gctcggacga cgccgcgcgg
      301 ctcgaagacg cggtgaccct gttcgcccgc gaggtcatgc cggcgtttag ctgaacacgg
      361 acgcccggat ggcggcgtca ttagaggaca accccatgcc cgtgaacctg atcgactaca
      421 tgcccttcgc ccgcgtgctg gggctcgagg tgacccattc ggacaaggag cgtgtaacgg
      481 cttccatcga ggtgagcgag gatctgtgca ccaccggcaa gatcatgcac ggcggggccg
      541 tgatggcggt ggcggacacc ctcggcgcca tcggcgccta cctgaacctg cccgacggcg
      601 ccaaggccac caccaccatc gagagcaaga ccaactttct aggtgcggcg cgtctgggca
      661 cccgagtcct ggcggagacg acgcccgtcc acgtgggcgg gcgcacctcc gtgtggcaga
      721 cgcgcctcac cggcgaggac ggcaagcccg tcgccctcgt cacccagacg cagttggttt
      781 tgagctgacc ggctgacggc ggcagtactt gggtctgccc aaccggtttc tggtttcccg
      841 ttatactcgc cgtcgctcct gtcaccaggt cgagcgttag tggccgacat cttcatctcg
      901 tacgcgcggt cgacccggcg ccaggcagag gccattggcg aggccctggt agagctcggt
      961 tactccatct ggcgagacga cgccctgccc gcccatcgcg cctacgccga cgagatccaa
     1021 aagcagctca acattgccaa ggcggtggta gtcgtctggt ccgcggatgc gatcaagtcg
     1081 gattgggtgc gctccgaagc cgaccgtgcg cgcaacgacc gcaagctcgt ccagctgacc
     1141 gtcgacgggg cagcgctgcc catgccgttc gatcagattc agtgcgccaa cctcgccggc
     1201 tggaggggag aaggccgggc cgccggctgg gcgacggtgc tggagagcat tcgtgcgctc
     1261 accgatacag ccgggaaaga atcaccggtg tctgcgggca cgcaatcgca gcagcagccg
     1321 gagcccatcc tcgccgttct gcccttcgac aacctctcca ccgatccgga gatgcagttt
     1381 ttctccgacg gggtcagcga ggagatcatt cagcggctgt cccggggggc gagcctcaag
     1441 gtcatcggcc ggacgtcgag ctttcagttt cgcggggagc gcaaggcgga agccgcctct
     1501 gcactcggct gcagccacat cctggacggt tcagttcgac gcggcgcggg gcggatgcgc
     1561 ctgagcgcgc acctcgtgga ggcgtcctcg ggcaccacgt tgtggtcgga gcgctacgac
     1621 cgttccctgg aggacgtgtt cgccgtccag gacgacgttt ccgagaacat cgccggggcg
     1681 ctgcatcaca cgtttcagag tttctcgacc cccgccgtgg acccaggcgc ctacgacctt
     1741 taccttcggg cgagccccga atcctacgcg cccgaggaaa tgcgcacgaa catcggcctg
     1801 cttgaagttg ccacccagcg tgcgcccgat tttcccgagg cctggggccg gctcgcctac
     1861 ctgcgagcct tcctgcggtt ctacgaaccg tttcccgggc gcggcgccag cgccgcgatc
     1921 atcgccagag aatcggccca tgctttacag gcggatcccg agaacggcga cgccctggcc
     1981 gcgagggcat tcgtggtcgc gcctttcgga aacttcatcg agctcgacac tgccgtcgag
     2041 cgcctgcgca gcgcgcccgg agacttcgac gcgagaaagt tcgccggttg gctgctgagg
     2101 aacttcggcc gggttcgcga aagcctcgcc gaggacgagc gcgcctacct cggcaacgcc
     2161 ctcgacccca tgtgcgccaa tactctggct ctggcccgac tggcagcggg acgcgtcgcg
     2221 gaagcgctgc cggtcctgga gaatctggtc gagcgcgttc ccgacatggt ttttcctttc
     2281 gccaacctgt tgcgtgccca ggccctgctc gagaactggg aggcgctgga cgagctgctc
     2341 gagctcaatg ccgaccgtcc cttgcgggaa tttcaggaca ctctggactt catacgcacc
     2401 aagcggaacc cgtcggcgga gaacatcgcc gtctggaaga gccagttcga ggcgcacatc
     2461 gcgaggacag gctgcatcga catctcccgc gtcgtctacg ctgcccatct cggcctggtc
     2521 gacgaagcct accaggccac cgagcaggcc cacctgggac ccaccggcac gaacgaagac
     2581 gtcatgggcc cggacggcta ccgcacgtcg ctgctgtttc aggcggccat gcccgagctg
     2641 cgcaacgacc cgcgcttcgt acccctgtgc gcccggctgg ggctggtgga gttctggaca
     2701 tccaccggca agtggccgga ttgcgccgac gaggtgccct acgacttccg cgccgcctgc
     2761 gaggatgcgc gcggcatccc caagctggag ttcggtttct gagacgcagg cccagacgcg
     2821 cgggactgcc agcgcagcgg gtgccgctag gaaccgatat tgtcgcgcag atagcggtcg
     2881 atgaagttgt ggaactgctg cacccgattc tcctgacctg agagatacgc gcccgcaaat
     2941 cccctcgact gcactccgag ctgctggcct gaggcgcgct cgagatcctg atcggccacc
     3001 tcgctcaacg tctcctggcc gtactccacc acccgccgct cggcctcctc ccagggcacc
     3061 atcccttccg gcccgggcac caggtccgag tctgcgccct tcagcgccag gaaccagtgc
     3121 tcgtagatgc tccgtgtggg gtccgtgggg tgcggctcgc agcgctgcat ctgggccccg
     3181 tccgccacca cggtgagcga ggtgccggga aagaggttga agtgataggc atccgtcagc
     3241 caggacgccg gcagacgctc gaagtagcgg tagcccctcg ccggccccag cttgcgcctc
     3301 tgcgcctgca gagcctcccg cacggcgtgc gcgcgcccct cgaaggcggc ggggtcaagc
     3361 ccccagttct gcatgtcggc ggcgagggtt tcgttgatgg cgaacggctc gtcgtaacgc
     3421 agcgacggca gcgagccttt catcttcatc aggttgtggc cggtctcgta gagctcgaag
     3481 tcagtgttcc ggtagtcgtc gtcgtagtag tcgctgatct gcgggtgggt ggccggcaga
     3541 tggtacgact cgcagaagtt gtcgtgcagc agcttccagt tgaagccgac ctcgcagacc
     3601 ttgtagtaga tgcgcctggc agccgaaaga tcgtgcaggt cgacgtagtc ctttacctcg
     3661 ccgagaaact ctgcgagcga ggcgcattcg gcgtccatgt tgtaccagac gaacccggcc
     3721 cagacctcgc aggggatctc tgccagcgtg agcttcccgc acgggttccc ccggggaaag
     3781 tcctcctcgt cctgcacgtg aacgaccgtc ccggcctggt cgaactgcca gccgtgatag
     3841 ctgcaggtga agcgctccac ggatccccgg tccgagtaga ccagccggtt gccgcggtgg
     3901 gggcagacgt tgtggaaggc acggaccgag ccgtccgcct gccgcaccat gagtatcgac
     3961 tcccggccga tcgcgtgggt gacgaagtca cccgccaccg ggatctcccg cgcccaggcc
     4021 ccgatgttcc agacccgggg ccacaagcgg tcgagctcca gctccatgaa ctcgcgggag
     4081 gtgtatcgct cgccggtgat ggtaacgtca gcgggaaaga tccggctcac gctccccgat
     4141 acggcttggc tgcgctgaac gagcgcttcc tgtctggacg taaccatgtc gctccccttc
     4201 tccaacctgg cgcggccgct tgcacgacaa ccctcttccg cttgccgtgc gtcaacgcgt
     4261 ccaacgatta ttacagccac cacagaga
//
LOCUS       DBNHKV010000225         9009 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1236007, whole genome shotgun sequence.
ACCESSION   DBNHKV010000225 DBNHKV010000000
VERSION     DBNHKV010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9009
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1236007"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="AC16PP_07140"
     CDS             complement(<1..773)
                     /locus_tag="AC16PP_07140"
                     /inference="COORDINATES: protein motif:HMM:NF028194.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR6481439.1"
                     /translation="MADVNRQVVLASRPDGYPVDSDFRVEEAGVPDVGEGQFLLRNAY
                     VSLDAGFRNWMNEDSGDDVLPAMALDAPVMGLVLGQVVESRHSEYPVGEWLMARLAWE
                     EYSISDGSDFISRLPEPRECPLPHYLGILGDTGLSAYFGLLDIGRPQPGETVLVSAAG
                     GAVGNVVGQLAKIHGAKAVGLAGTDEKCARLVEELGFDAAVNHRSPDLAGVIAAACPD
                     GVDVFFDSVGGPLLETVLWQIANGARIVMCGAVAAYNAL"
     gene            complement(773..1807)
                     /locus_tag="AC16PP_07145"
     CDS             complement(773..1807)
                     /locus_tag="AC16PP_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6481440.1"
                     /translation="MLDRTDRRRFVRQLGLLGVAGQTLLRSGSLRAAVGEARDSAALD
                     SAWPEMAYRRLGRTGFEGSRLIFGCGAALMDRPRDDLLERAFEAGVNVFDSGTSRYYD
                     NAEKNLAPFLKRHRDEIFLISKAMVYLDVAPDEQITVAQARQAAQTWTGLMDESLKEL
                     EVDRIDAYYLMASNNPTVVGSEEINEAFQRARQAGKVRYLGLSTHQNAENVLKTAIET
                     GWYDLAMIAITPGGWYDWENRSVLEGSPTMVELQPLLAKASEAGIGLIGMKAGRFLAG
                     RKWLGWGNPEAFDGHYDQSFLRAQLSAFQRSYAYVLAHGLDAVNADMQNFAHLKENFV
                     AAATSHRYFA"
     gene            1910..2689
                     /locus_tag="AC16PP_07150"
     CDS             1910..2689
                     /locus_tag="AC16PP_07150"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6481441.1"
                     /translation="MANILDQFKLDGQVAVVTGGGRGIGEGIALGMAEAGADIVVAAR
                     RTEEIEAVAEKVRALGRRALAVTTDMMQIEQVQALAQRAFEEMGKLTCWVSNAGGADD
                     RVPRTLLEMPERQWDFQLNLNLKAVWTGAQAAANIMKDHGGGTIINISSTAANRASPF
                     NGPYGVAKSGVNNLTQTLGAELAQFGIRVNGVSPGPIPTEVFMEFFGITEDDIPKLGE
                     RFEIPLGRVGTPEDIATAVIFLASRASSWMTGETFLVNGGR"
     gene            2739..4406
                     /locus_tag="AC16PP_07155"
     CDS             2739..4406
                     /locus_tag="AC16PP_07155"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6481442.1"
                     /translation="MSKADLVIRNATVVDGTGNPAVTGDVAVKDGRILAVGDFGGEAT
                     REIDASGLVLAPGFIDIHTHFDPQLCWDGLATPSIEHGITTVVIGNCSLSLAPVKGDG
                     RDKIVNMFGVIEDIKKPTFDAAVPFSWESFEEYLDFIRPNLGINVAALIGHSALRFYV
                     MGAQSQERAATADEIEEMCRLVEEAMAAGAAGISSSYVDIDENMNPVPSRLADLDEKI
                     ALAKAMAKSGRGIWQVVPYFPDMQQQLANIRELGEISLAAGVPCSIQPVLSSPTDPTA
                     SEILTALEAERERGARVYGQVMPRCFDMNMRLSETSMLLYALPKWKAIMDMPRSERRA
                     RFEDPAMRTELVEEMRNAQGMSMALPMLTVGRVQAEANQPYQGRMLAEIAQAEGKDLG
                     EVILDLSLADDLDAEFRLQNVLNADKAAVADLIDHPLLHFGASDAGAHITQFCGTGDT
                     THLLEHFVRDSGRLTLERGIHRMTGEVAADWGLADRGTIEAGKAADLVLFDLNRIHCR
                     DEEFVDDFPGEARRYIRRADGYTAVLVNGEVVLENGTYTDARSGSIV"
     gene            4497..5477
                     /locus_tag="AC16PP_07160"
     CDS             4497..5477
                     /locus_tag="AC16PP_07160"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6481443.1"
                     /translation="MQYTNLGPTGVRVSRLCLGMMTYGSKSWRDWVLDYDEAKPFVEQ
                     ALEAGINFFDTADVYSNGASEEVLGRALTELSVDREDVVIATKCFNGTHERRRNRWGL
                     SRKHILSACDASLRRLGTDYIDLYQIHRFDYRTPIEETIDALSHLVRVGKVRYLGASS
                     MFAWQMAKYLFTADAMGGVRFVTMQNHYNLIYREEEREMIPLCIDQGVGLIPWSPIAR
                     GYLARSREAQHDTLRAGSDEFARMLYQRESDLDIIDRNAEVAAGLGVKPAQTAIAWML
                     GKPGIVAPIIGASRPEHLADAAAAVELTLSEDDVSRLEALYEPHAVSGHA"
     gene            5527..6675
                     /locus_tag="AC16PP_07165"
     CDS             5527..6675
                     /locus_tag="AC16PP_07165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6481444.1"
                     /translation="MKFGIFFELSTPRPLTREHEWAVYHNALEQCRLADELGFDHVWA
                     VEHHFLEEYSHCSAPEVFLTAVAAQTRRVRVGHGAVVCVPQMNHPVRVAERAAALDIV
                     SNGRLEFGTARSSTWTELGGFEVNPDDTKKTWDEFVRAIPKMWMQEEFEWEGKSFSMP
                     ARNVLPKPLQDPHPPMWVTVTSPGTEIDAAERGLGCLGVSSAGFEEQERRTQAYHRLV
                     SQCEPVGGVINDQVHTMNFLYCHEDYRKARDVGVPMVTAFNVANAHLYWTREAYPTRA
                     YQTLGNAGSAMRAKKPAADDPSVAKGLPEGLGVGDPDHLIRTIKTWESIGVTGINFLL
                     NAMEMVPQEEVLESMRLFAREVMPHFKEPDERARPVWSPAGLPQVAAG"
     gene            6675..7427
                     /locus_tag="AC16PP_07170"
     CDS             6675..7427
                     /locus_tag="AC16PP_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481445.1"
                     /translation="MLTGNADLAELAAGAPTIAAFPTEPVQLEDVECFQLTAEMRSSA
                     RESVLPAGLHPTIPAALSVQAWKVGASPWGAFAVCFCRVSCRSGVRARGFSTAAVVQG
                     DAALSALRDAFGFPARPGAVNLGRHYDGVDLTVSDESGTTLEVAALDPDPLGLEDVQY
                     TGTMNLAHTPQGLRLVQVESGHSATRVERLSARILHFDGASWGNERLAPYRVIAASVA
                     LEDIVLPPVRFVCKPDEPAFTGTEPVGEPSGK"
     gene            complement(7441..7920)
                     /locus_tag="AC16PP_07175"
     CDS             complement(7441..7920)
                     /locus_tag="AC16PP_07175"
                     /inference="COORDINATES: protein motif:HMM:NF018049.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated ZapG family protein"
                     /protein_id="HIR6481446.1"
                     /translation="MYSLAWLVVVGVTAAVAGVAMGYLVAQVRSRSRERIRDLEAELS
                     KAQDELAGYRREVFEQFAETARKFKTLNESYSDLHRQLASSASLLCGEAAGPLLAAPP
                     RGAEITAQAEPSPEAEVEPPIVVAEVEDPATGADPQPGDEDSLAAQDGAVPRAREAG"
     gene            complement(8015..8488)
                     /locus_tag="AC16PP_07180"
     CDS             complement(8015..8488)
                     /locus_tag="AC16PP_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020799109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6481447.1"
                     /translation="MSNEALQNAYETLSARIGKSSEPTDWFEITQERINSFADATMDH
                     QWIHIDPERAAAGPFGTTIAHGQLTQSIMSFLPGGEGVGLPALDGMQMGINYGWNRVR
                     FPSPVPVGANIRTSAAIKSVEIKGNMLEVVNELTVEVKGQDKPACVAESVLRLVF"
     gene            8628..>9009
                     /locus_tag="AC16PP_07185"
     CDS             8628..>9009
                     /locus_tag="AC16PP_07185"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6481448.1"
                     /translation="MAKPFPKHPHLEGGFAPIHMECDAPDLVVEGEVPRELNGVFYRN
                     GPNAQFAPRGDYHLFAGDGMIHGFYIRNGQVAYKNRWVRTVKWTREHEAGGALFSAFN
                     PMDSDESVRGMETDGLANTNVVWHG"
BASE COUNT         1624 a         3042 c         2866 g         1477 t
ORIGIN      
        1 tccagggcgt tgtaggcggc caccgcgccg cacatgacga tcctggcgcc gttggcgatc
       61 tgccagagca cagtctccag cagcgggccg ccgacgctgt cgaagaagac gtcgacaccg
      121 tcgggacagg ccgccgcgat gacaccggcc agatccgggc ttcggtgatt gaccgccgcg
      181 tcgaagccga gctcttcaac gagacgcgcg cacttttcgt cggtaccggc caggcccacg
      241 gctttggccc cgtgaatctt cgcgagctgc cccaccacgt tgccgacggc tccgcccgcg
      301 gcgctcacca gcaccgtctc accgggctgc ggccggccga tgtccagcaa gccgaaatag
      361 gccgagagcc ccgtatcacc cagaatcccc aggtaatgcg gcagcgggca ctcgcgaggt
      421 tccggcagcc gcgaaatgaa gtcggagccg tcgctgatcg agtactcctc ccaggccagc
      481 ctggccatga gccactcgcc taccggatac tcggagtgcc gtgactcgac cacctgcccc
      541 aggacgagcc ccatgacggg cgcgtccagc gccatggccg ggaggacgtc gtcgccgctg
      601 tcctcgttca tccagttgcg gaaacccgca tccagggaga cgtaggcatt gcgcagaaga
      661 aactgccctt ccccgacgtc gggtacccct gcctcctcca cgcggaagtc actgtcgacc
      721 ggatagccgt cgggccgcga cgccagcacg acctgtcgat tgacgtcggc catcaggcga
      781 agtagcgatg agaggtggcc gcggccacga agttctcttt gaggtgcgcg aagttctgca
      841 tgtctgcgtt caccgcatcg agaccgtggg ccagcacgta agcgtagctg cgctggaaag
      901 cggacagctg ggctctcaag aaactctgat cgtagtgccc gtcgaacgcc tcggggttgc
      961 cccagccgag ccatttccgg ccggccagaa agcgcccggc cttcatgccg atgagcccga
     1021 tgcccgcttc gctcgctttg gcaagaagag gttggagctc caccatggtg ggcgagcctt
     1081 ccagcaccga gcgattctcc cagtcatacc agccgccggg ggtgatggcg atcatggcca
     1141 ggtcgtacca gcccgtttcg atggccgttt tcagcacgtt ttccgcgttc tgatgggtgc
     1201 tcaaacccag gtagcgcacc ttgcccgcct gccgcgcgcg ctgaaacgcc tcgttgatct
     1261 cctcgctgcc gaccacggtc gggttgttgg acgccatgag gtaataggcg tcgatgcggt
     1321 ccacctccag ctccttcagg ctttcgtcca tcaggccggt ccaggtctgt gcggcctgac
     1381 gcgcctgggc cacggtaatc tgctcgtcgg gcgccacgtc gaggtagacc atggcctttg
     1441 agatgagaaa gatctcgtct cgatgccgct tcagaaaagg ggcaaggttc ttctccgcgt
     1501 tgtcgtagta gcgcgacgtt cccgagtcga acacgttcac gcccgcctcg aaggcgcgct
     1561 cgagcaggtc gtcccggggg cggtccatga gcgccgcccc gcagccgaat atgaggcggc
     1621 tcccctcgaa gcccgtgcgg ccgagcctgc ggtaggccat ctccggccag gccgagtcga
     1681 gcgcggccga atcgcgcgct tcgcccaccg ccgcgcgcaa gctccccgag cgcagcagcg
     1741 tctggccagc cacgccaagc agcccaagct ggcgaacgaa tctccgacgg tcagtgcggt
     1801 cgagcatgtc acggctcccc tgccgattag cgatccgctc attctaggcg atcgcccgga
     1861 caatttgggt atactgctgc gctttgactt ttcccggggg caaggggcaa tggcgaacat
     1921 actcgaccag ttcaagctag acggtcaggt tgcggtggta accggcggcg gacgaggcat
     1981 cggcgaaggc atcgcgctgg gcatggcgga agcgggtgcg gacatcgtgg tcgcggcacg
     2041 tcgcaccgag gagatcgaag cggtggccga gaaggtcagg gctcttggtc gacgggctct
     2101 ggcggtcacc acggacatga tgcagatcga gcaggttcag gctctggctc agcgtgcgtt
     2161 cgaggagatg ggcaagctga cctgctgggt cagcaacgcg ggcggcgccg acgaccgggt
     2221 gccccgcacg cttttagaga tgccggaacg gcaatgggac tttcagctga acctgaacct
     2281 caaggcagtc tggaccggtg cccaggccgc agcgaacatc atgaaggacc acggcggcgg
     2341 gacgatcatc aacatctcgt ccacggccgc caaccgcgcc tcgcccttca acggccccta
     2401 tggggtcgcg aagtcgggcg tgaacaatct cacccagacc ctcggcgcgg agctcgcgca
     2461 gttcggcatc cgcgtcaacg gggtctcgcc aggccccatc cccaccgagg tgttcatgga
     2521 gttcttcggc atcaccgaag acgacatccc gaaactgggc gagcgtttcg agataccgct
     2581 cggccgcgta ggcacccctg aagacatcgc caccgccgtg atctttctgg cgtcccgtgc
     2641 ctcctcctgg atgacgggag aaactttcct ggtgaacggc ggccgttgat ccaaggagcc
     2701 cggtcgactg agcgatcgct aatcatgagg aaattcggat gagcaaagct gatctggtca
     2761 tacgcaacgc taccgtcgta gacggcaccg gcaacccggc agtcaccggg gacgttgccg
     2821 tgaaggacgg caggatactc gccgttggcg acttcggggg cgaggccacc cgcgagatcg
     2881 acgcctccgg cctggttctg gctcccgggt tcatcgacat ccacacccac ttcgacccgc
     2941 agctttgctg ggatggcctc gccacgccat ccatcgagca cggcatcacc accgtggtca
     3001 tcggcaactg ctcgttaagc ctcgcccccg tcaagggcga cggccgcgac aagatcgtca
     3061 acatgttcgg ggtgatcgag gacatcaaga agccaacatt cgacgcggcg gtgccgttca
     3121 gctgggagag cttcgaggag tacctggact tcatccggcc caatctcggc atcaacgtgg
     3181 cggcgctcat cggccactcc gccctgcgct tctacgtgat gggcgcacag agccaggagc
     3241 gggccgcaac cgccgacgag atcgaggaga tgtgccggct ggtggaagag gccatggccg
     3301 ccggcgcggc cggcatctcc tcgtcttacg tggacatcga tgagaacatg aacccggtcc
     3361 ccagccggct ggccgacctg gacgagaaga ttgcgctggc caaggccatg gccaagagtg
     3421 gtcggggcat ctggcaggtg gtgccctact tccccgacat gcagcagcag ctggccaaca
     3481 ttcgagagct cggcgagatc agcctggccg ccggcgtgcc ctgttccatt cagcccgtgc
     3541 tttcctcgcc gacagacccc acggccagcg agatcctcac ggcactggag gcggaacggg
     3601 agcgcggcgc ccgcgtctac ggccaggtca tgccccgctg cttcgacatg aacatgcgtc
     3661 tttcagagac gagcatgctg ctgtatgccc tgcccaagtg gaaggccatc atggacatgc
     3721 ctcgcagcga gcgccgcgca cgtttcgagg atcccgccat gcgtaccgag ctggtggagg
     3781 agatgcgcaa cgcccaaggc atgagcatgg ccttgccgat gctcaccgtg ggccgggtgc
     3841 aggccgaggc caaccagcct tatcaaggca ggatgctggc agagatcgcg caagccgagg
     3901 gcaaggatct cggcgaggtg atcctggatc tgtccctggc agacgatctg gacgcggagt
     3961 tccgcttgca gaacgtgctc aacgccgaca aggcagccgt cgccgacctc atcgaccatc
     4021 cgctgctgca cttcggcgcc tccgacgccg gcgcccacat cacccagttc tgcggtaccg
     4081 gcgacacgac ccatctcctg gaacacttcg tccgggacag cggcaggctc accctggagc
     4141 gcggcattca ccgcatgacg ggagaggtgg ccgccgactg gggccttgcc gaccgcggca
     4201 ccatcgaggc aggcaaggcg gccgatctgg tgctgttcga cctgaaccgc attcactgcc
     4261 gggacgagga gttcgtggac gacttccccg gcgaggcgcg ccgctacatc cgccgggcgg
     4321 acggctacac ggcggtgctc gtcaacggcg aggtcgttct ggagaacggc acctacaccg
     4381 acgcccgcag cggcagcatc gtctaggcgc gaccgcgcac gcgccggccc cacggccggc
     4441 gccctttacc gagcccgcgt ctctgcctac agttgcagag tcaccgggag agaacgatgc
     4501 aatacaccaa tctgggtccc acgggcgtgc gcgtgtcgcg cctctgcctc ggcatgatga
     4561 catacggctc gaaatcctgg cgcgactggg tgctcgacta cgacgaggcg aagcctttcg
     4621 tcgagcaggc cctggaggcc ggcatcaact tcttcgacac cgccgacgtc tactccaacg
     4681 gcgcatccga ggaggtgctg ggccgcgccc ttacggagtt gagcgtggac cgcgaggacg
     4741 tcgtcatcgc caccaagtgc ttcaacggca cccacgagcg gcgtcgcaac cggtggggac
     4801 tctcacgcaa gcacattctc tcggcctgcg atgcctcact gagacgactg ggcaccgact
     4861 acatcgacct gtatcagata caccgtttcg actaccggac ccccatcgag gagaccatcg
     4921 acgccctctc ccatctcgtg cgggtgggca aggtgcgcta cctgggcgca tcgagcatgt
     4981 ttgcctggca gatggccaag tacctcttca ccgcggacgc catgggcggc gtgcgcttcg
     5041 tgaccatgca gaaccactac aacctgatct acagagagga agagcgggag atgatcccgc
     5101 tgtgcatcga tcagggcgtc ggcctgatcc cgtggagccc catcgcccgg ggctacctgg
     5161 cccgcagcag ggaagctcag cacgataccc tgcgcgcagg gtcggacgag ttcgcaagaa
     5221 tgctctacca gcgcgaaagc gacctcgaca tcatcgaccg caacgcagag gtggccgccg
     5281 gcctgggcgt gaagcccgcg cagacggcca tcgcgtggat gctgggcaag ccaggaatcg
     5341 tcgcgcccat catcggcgcg tccagacccg agcacctggc ggacgccgcc gccgcggtag
     5401 agctgacgct ttccgaggat gacgtgtccc ggctcgaagc gctctacgag cctcacgccg
     5461 tgtccggaca cgcgtaaagg cagttctgct tcggttacac cacttgacga aaggatcatc
     5521 caagccatga agttcggcat cttcttcgag ctgtccacgc cgcggcctct tacccgggag
     5581 catgaatggg ccgtctacca caacgccctg gagcagtgcc ggctggccga cgagctcggc
     5641 ttcgaccatg tctgggccgt ggagcaccac tttctggagg agtactcgca ctgctccgcg
     5701 cccgaagtct ttctcaccgc cgtcgccgcc cagacccgcc gcgttcgcgt cggccacggc
     5761 gcggtggtgt gcgtccccca gatgaaccac cccgtcaggg ttgccgagcg ggcggccgcc
     5821 ctcgacatcg tctccaacgg caggctcgag ttcggcacgg cacgctcctc cacctggacg
     5881 gagctgggcg gcttcgaggt caaccccgac gacaccaaga agacctggga cgagttcgtc
     5941 cgcgccatcc cgaaaatgtg gatgcaggag gagttcgagt gggagggcaa atcgttctcc
     6001 atgcccgccc gcaacgtcct gcccaagccg ctgcaggatc cccatccgcc catgtgggtc
     6061 acggtgacca gccccggcac ggagatcgac gcggccgagc gcggcctcgg ctgtcttgga
     6121 gtatcgtcgg ccggattcga ggagcaggag cgccgcaccc aggcctatca ccgcctggtg
     6181 agccaatgcg aaccggtggg aggcgtgatc aacgatcagg tccacaccat gaacttcctg
     6241 tactgccacg aagactaccg caaggcccgg gacgtcggcg tgcccatggt gacggcgttc
     6301 aacgtcgcca acgcgcacct ctactggacc cgggaggctt acccgacccg cgcttaccag
     6361 acgctgggca atgccggatc cgccatgcgg gcgaagaaac cggcggccga cgatcccagc
     6421 gtggccaagg gcttgccgga agggctcggc gtgggcgatc ccgaccatct gattcgcacc
     6481 atcaagacgt gggagtccat cggcgtgacg ggcatcaact tcctgctcaa cgccatggag
     6541 atggtgcctc aggaggaggt cctggaaagc atgcggctct tcgcgcgcga ggtcatgccg
     6601 cacttcaagg aacccgacga gcgggcgcgg cccgtctggt cacccgccgg gctcccgcag
     6661 gtggcggccg gctgatgctg accggcaacg ccgacctggc cgagctggcg gcgggagccc
     6721 cgaccatcgc cgcatttccc accgagccgg tgcagctgga ggacgtcgag tgtttccagc
     6781 tcaccgccga gatgcgctcg tccgcccggg agtccgtgct gcccgcgggt ctgcacccca
     6841 ccattccggc ggcgctttcc gtgcaggcat ggaaggtggg cgcaagcccg tggggagcgt
     6901 tcgccgtctg cttctgccgg gtcagctgcc gcagcggcgt gcgcgcccgg ggtttttcca
     6961 ccgctgcggt ggttcaagga gacgccgcac tgagcgcgct gcgggacgcc ttcgggtttc
     7021 cggcccgccc cggcgcggtg aacctgggtc gacactacga cggcgtggat ctcaccgtca
     7081 gcgacgaaag cggcacgacg ctcgaggtcg ccgccctgga ccccgatcct ctgggcctgg
     7141 aagacgttca atacacggga accatgaacc tggcccacac gccccaaggc ctgcggctcg
     7201 ttcaggtgga gtccgggcac tcggccacac gggtggagcg cctgagcgcg cgaatcctcc
     7261 actttgacgg cgcctcctgg ggcaacgaac gcctggcacc gtaccgggtg attgccgcat
     7321 ccgtggcgtt ggaggacatc gtgctgccgc cggtgcggtt cgtgtgcaag ccggacgaac
     7381 cggcgttcac cggcaccgag ccggtaggcg agccgtcagg aaagtaagct tgaaaggagg
     7441 ctagccggct tcgcgcgcgc gcgggaccgc cccatcttgc gcagcgagtg aatcctcgtc
     7501 gcctggctgc gggtcagcgc ccgtcgcggg gtcttctact tccgcaacga cgatgggcgg
     7561 ttcgacttcc gcttcgggcg acggctcggc ctgggcggtg atttccgccc cccgcggcgg
     7621 cgcggcaagc aacggcccgg ccgcttcccc gcagagcagg ctggcgctgg aggccagctg
     7681 acggtgcaga tcgctgtagg actcgttcag ggtcttgaac ttgcgcgcag tctccgcgaa
     7741 ctgctcgaaa acttcccgcc ggtagccggc aagctcgtcc tgagccttgc tcagctccgc
     7801 ttccagatcc cggatccttt cccgagaccg actgcgcacc tgcgccacga gataacccat
     7861 agcgacgccg gctacagccg ccgtcacgcc caccaccacg agccaagcca gggaatacat
     7921 cgaccgttac ctcgcactac gtcgccagac cgatgggcgc attctactcg gagttcgagc
     7981 tcaaaacctc aaggggtgca ggggcgcggg gtactcagaa gaccaggcgc agaacgctct
     8041 ccgcgacaca cgccggcttg tcctgcccct tcacctcaac ggtgagctcg ttgacgactt
     8101 ccagcatgtt gcctttgatc tcgacgctct tgatcgccgc gctcgtccgt atattggccc
     8161 caaccggcac cggggacgga aagcgcaccc gattccagcc gtagttgatg cccatctgca
     8221 tgccgtcaag cgccggcagc cccacacctt caccgcccgg aaggaagctc atgatcgact
     8281 gcgtcagctg gccgtgagcg atcgtggtgc caaacgggcc cgccgcagca cgttcggggt
     8341 cgatgtggat ccactggtga tccatggtgg cgtcggcgaa cgagttgatg cgttcctgcg
     8401 tgatctcgaa ccagtcggta ggttctgaag acttgccgat tcgggcactt agcgtctcgt
     8461 aagcattctg cagagcttcg ttcgacatgt gatttccccc aggttgaaag agcgccgatt
     8521 atctacgccg cgccgaatca tgtcatgcgc gcccgagcag agcccggaca tcgctcgcgc
     8581 aaagggcgtc aaaccctcta tagttgagca aacacaggag gtggcccatg gctaaaccat
     8641 tcccgaagca tcctcacctg gaaggcggct ttgcccccat tcacatggag tgtgatgccc
     8701 ccgatctggt cgtcgagggc gaggtgccac gcgagctgaa cggcgtcttc tatcgcaacg
     8761 ggccaaacgc tcaattcgcg ccgcgcggcg attaccacct ctttgccggg gacggcatga
     8821 tccacggctt ctacattcgc aatggccagg tggcgtacaa gaaccgctgg gtgcgtaccg
     8881 tcaagtggac cagggaacac gaagccggcg gggcgctctt cagcgcgttc aaccccatgg
     8941 actccgacga gagcgtacgc ggcatggaga ccgacggcct ggcaaacacc aacgtggtgt
     9001 ggcatggcg
//
LOCUS       DBNHKV010000226         3101 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_953673, whole genome shotgun sequence.
ACCESSION   DBNHKV010000226 DBNHKV010000000
VERSION     DBNHKV010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_953673"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="AC16PP_07190"
     CDS             <1..626
                     /locus_tag="AC16PP_07190"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6481449.1"
                     /translation="AMLLAHMHYTGEGVERDLDRATDYYVASAESGNDFARRSYARFL
                     LAEAREDPRALQWLEELAEDDDAEAMILLGNLHARGVGVKQSLRRAVGWYKDAVDASG
                     GDANIVNEVAWTLTVSDLEGLKRTRYALNIMEDLMTRDDEARRKPEYLDTWAATYAAN
                     GDFIRAVAVQEEAVEAAEDDQYEGVREIIKTHLDAFKSGKTITETAP"
     gene            623..1654
                     /gene="mnmH"
                     /locus_tag="AC16PP_07195"
     CDS             623..1654
                     /gene="mnmH"
                     /locus_tag="AC16PP_07195"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HIR6481450.1"
                     /translation="MNGTDPETLLAEGARFIDVRAPSEFTRGAVPGAINLPILDDDER
                     HEVGSTYNNEGHNAAVALGYRLVSGAVRQARVAAWAEALDAYPEAWIYCWRGGQRSQI
                     AQEWLHEAGYEVPRVEGGFKALRQCCLEVLARAPHALPWLVLAGRTGSGKTVLIRERP
                     DSIDLEGQANHRGSAFGGRSCTQPSPISFENALAVAFLDLDSQRLIVEDESRTIGRLA
                     LPAAWHERMQTAPVVVVEADLSERVENIRREYVDEPLAEGVTPGDLQARYQGALERIS
                     RRLGGDRCRAVARALAEGFASGHHHAWIEQLLLWYYDPMYDYQLSKKQDRIVMQGDRD
                     AVREFLATP"
     gene            complement(1693..>3101)
                     /locus_tag="AC16PP_07200"
     CDS             complement(1693..>3101)
                     /locus_tag="AC16PP_07200"
                     /inference="COORDINATES: protein motif:HMM:NF036578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-rhamnosidase-related protein"
                     /protein_id="HIR6481451.1"
                     /translation="GFRYVEVCGDLADDEPGVVAVAVNTDLDATATFESDHPLLDRLH
                     ANIVANQRGCALDVPNAGFSLERRIPLTGEARAFLGTAAMNLDVAAFLAKWLTDLADA
                     QLPDGGFPSVAPVPPLVPALQQDGGPGWSDAFAACAWILYRHFGDRRVLERNYPALRT
                     YVLGQAAAWPDCLARPGSHPLGPADRGAVASLKATALFLQAVRLASRIAGVLGNLSDF
                     EQFDGLAQSIRSAFRNRFVTHDGLVVGDCVTSYTLALHLGLLERGERRAAFEELVAKL
                     EDGEPPAVEPLAAPYLLGVLTRGGRVDLAYLVLLGSRPGSWLYPVLHGASSLWDGETE
                     SSRLARGSVGEWLYTGLAGLDLDDDLSETNNAFRRIRIQPHPPLRDGIELAGNVPPMR
                     RVKASLDTVHGRYESAWEILEDGFVLRVQVPGNCSALVVMPDGTEHEVVAGRHEFKQD
                     FLGETDEIPILREIAEAS"
BASE COUNT          581 a         1070 c         1012 g          438 t
ORIGIN      
        1 ccgccatgct gctcgcgcac atgcactaca ccggcgaggg cgtcgagcgc gatctggacc
       61 gcgcgacgga ctactacgtc gcttcagccg agagcggcaa tgacttcgct cgtcgcagct
      121 acgcgcgttt cctgctggcc gaggcgcgcg aggatcccag ggccctgcag tggctggagg
      181 aacttgctga ggatgacgat gcggaggcga tgatcctgct gggcaacctc cacgcacgcg
      241 gcgtcggggt aaaacagagc ctgcggcgcg cggtcggatg gtacaaggac gccgtggacg
      301 cgtccggggg cgatgcgaac atcgtgaacg aggtggcatg gacgctgacg gtcagcgacc
      361 tggagggcct gaagcgaaca cgctacgcgc tcaacatcat ggaagatctc atgacccgcg
      421 acgacgaggc gcgccgcaag ccggagtacc tggacacctg ggcggcaacc tatgcggcca
      481 acggcgattt catccgcgcc gtggcggttc aggaggaagc cgtcgaggcg gcagaggacg
      541 accaatacga gggcgtgcgg gagatcatca agacccatct ggatgccttc aagagcggaa
      601 agacgatcac cgaaaccgcg ccgtgaacgg aaccgatccg gaaacgctgc tggctgaagg
      661 cgcgcgcttc atcgacgtca gggcgccctc cgagttcacc cggggcgccg tacccggcgc
      721 catcaacctg ccgatcctgg atgacgacga gcgccacgaa gtcggctcca cctacaacaa
      781 cgagggtcac aacgctgccg tcgccctggg gtaccgcctg gtgagcggcg ccgtgaggca
      841 agcccgcgta gccgcctggg ctgaggccct agacgcgtac cccgaggcgt ggatctactg
      901 ctggcgtggg ggccagcgct cccagatcgc acaggagtgg ctgcacgaag cgggctacga
      961 agtcccccgg gtcgaaggcg gcttcaaagc tctgcggcaa tgctgcctgg aggtgctggc
     1021 cagggcgccg cacgcgctgc cctggctggt cctcgccggg cggaccggct cgggcaaaac
     1081 cgtgctgatt cgtgagcggc cggacagcat cgatctggaa ggccaagcca accatcgcgg
     1141 ctccgcgttc ggcggcagat cgtgcacgca accatccccg atcagcttcg agaacgctct
     1201 ggcggtggcc ttcctcgatc tcgactccca gcgattgatc gtggaggacg aaagccggac
     1261 catcggccgc ctcgccctgc cagccgcttg gcatgagcgc atgcagaccg cgcccgtggt
     1321 ggtggtggaa gccgacctgt ccgagcgggt ggagaacatc cgccgcgagt acgttgacga
     1381 accgcttgcc gagggcgtga cgcccggcga cctgcaggcc cgctaccagg gagcgcttga
     1441 gcgcatcagc cgccgcctcg gcggcgaccg ctgtcgggcc gtggcgcgcg ccctcgcgga
     1501 gggcttcgcc tccggccacc accacgcctg gatcgagcag ctgctgctgt ggtactacga
     1561 ccccatgtac gactaccagc tgagcaagaa gcaggaccgc atcgtcatgc agggcgaccg
     1621 cgacgcggtc cgggagtttc tcgccacacc ctgaccggct gccccacggc ggcccaaccc
     1681 cgccgcaggg aatcaagacg cctcggcaat ctcccggaga atggggatct cgtcagtctc
     1741 gccaaggaaa tcctgcttga actcgtgacg tcccgccacc acctcgtgct cggtcccgtc
     1801 cggcatgacg accagcgccg agcaattgcc cgggacttgg acgcggagca cgaacccgtc
     1861 ctcgaggatc tcccaggccg actcgtagcg cccgtgcacg gtatccagcg aagccttgac
     1921 ccgacgcatc ggggggacgt tgccggccag ctctatcccg tcacgtaacg gcgggtgagg
     1981 ttgtatccgg atgcgccgga aggcgttgtt ggtctccgat agatcatcgt ccagatcgag
     2041 cccggcaagc cccgtgtaca accactcgcc taccgagccc cgagcaaggc ggctcgactc
     2101 cgtttcccca tcccacagcg agcttgcacc gtgcagcacg ggatacagcc agctgcccgg
     2161 gcgcgagccg agcagcacca gataggccag atcgacgcgg ccgccccgcg tcagcacccc
     2221 aaggagatag ggtgcggcca gcggttccac tgcaggcggc tcgccgtctt ccagcttcgc
     2281 caccagctcc tcgaacgccg cgcgccgctc gcccctttcc agaagcccta gatgcagagc
     2341 aagggtatag ctggtgacgc agtcgccgac caccaggccg tcgtgggtca cgaagcggtt
     2401 cctgaaggcg gaacggatac tctgcgctaa gccgtcgaac tgctcgaagt cgctcagatt
     2461 ccccagcacc ccggcaatgc gcgaggcaag ccgaaccgcc tgaagaaaca aagccgtcgc
     2521 cttcagcgag gcaacggcac ctcggtcggc aggcccgagc ggatggctgc ccggcctggc
     2581 cagacagtcc ggccaggccg ccgcctgccc aagcacatag gtccgcagcg ccggatagtt
     2641 gcgttcgaga acccgccgat ctccgaaatg ccggtagaga atccaggcac atgccgcaaa
     2701 cgcgtccgac cagcccgggc ccccgtcctg ctgcagcgcg ggcaccagcg gaggcacagg
     2761 agcgacgctg gggaaccccc catccggcag ctgcgcatct gccagatccg tcagccactt
     2821 cgccagaaag gccgccacgt cgagattcat ggccgcggtg ccgagaaagg cacgcgcctc
     2881 ccccgtcagc ggaatcctgc gttccaagga gaagcccgca ttgggtacgt ccagggcgca
     2941 gccgcgctgg ttggcaacga tgttggcgtg caggcgatcc agcaggggat ggtccgactc
     3001 gaacgtggcc gtggcgtcca ggtcggtatt gacggctacc gcaaccactc ccggctcgtc
     3061 gtctgccaga tcgccgcaaa cctctacata ccggaagccg t
//
LOCUS       DBNHKV010000227         8078 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_881922, whole genome shotgun sequence.
ACCESSION   DBNHKV010000227 DBNHKV010000000
VERSION     DBNHKV010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8078
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_881922"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..963
                     /locus_tag="AC16PP_07205"
     CDS             79..963
                     /locus_tag="AC16PP_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6481452.1"
                     /translation="MKIRSAMVVMAKPKHKQFALERAKQLQQSCGARLRLVSFCWNAM
                     CEQSDVFEAHQRRAIKATIMRERESWLIDQVRDAGMAAADVSIEVQWTDNIAGWVSEK
                     AAANEFDLVLKSVNRSRTLVHTPLDWQLLEQCSKPVYISAPPRRSASGNVLAALDFRH
                     RDRKHQALNIRVLEAAQEFADMSGGKLHCVNVVEFSQVLSDLDLIDPRSVKKKALANN
                     QELMNALLEPFDVPKSRMHTPLGKVGQMIAITARKIDADLLVIGTSTRRKGGTVVIGN
                     SAQRTLEKSPCDLLAVHP"
     gene            complement(1043..1459)
                     /locus_tag="AC16PP_07210"
     CDS             complement(1043..1459)
                     /locus_tag="AC16PP_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6481453.1"
                     /translation="MLNTLKLILALAATCYFGLAAADDIAYDGGELYQANCANCHGMY
                     GEGDGIVTPSLSVVLQDLRYLAERNDGEFPRWFVTEIIDGRELRLAHGPDGMPVWGEA
                     FTRMEGYGEQAQSRVEQKIDALVNFIESIQIAPEDE"
     gene            complement(1462..3507)
                     /locus_tag="AC16PP_07215"
     CDS             complement(1462..3507)
                     /locus_tag="AC16PP_07215"
                     /inference="COORDINATES: protein motif:HMM:NF014312.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HIR6481454.1"
                     /translation="MTPAKPIKATLIGLFAVCPLISPMVAHPNTAEQEKTGKELYEQQ
                     CARCHQRDGGGVDGLYPSLRNAPSLWLDRKTAIRGVLAGRADDTVVNSPLMPTHGYLG
                     NETVASTLTFLLQEWGPGGQPYTAEEVAAERLELLASQPAPHPGLPDVSPLADMDAVQ
                     YVTSSGPPLSVSEFDRARSLYYGHCTGCHGVLREGTAGSPLTPVLMRQRGTEYLQAVI
                     NYGSSAGMFNWGTSAALTAEEINMLARFLQHPVPQAPDMTEADVRASWSLEVPLKDRP
                     GVPQGRHPLDELFVVTLHDVAQIALIHGPSRRVIGTVATGQSPHRVRGSASGRYLYVI
                     ARDGSLTMIDLYARKPRRVASVRVGYEARAVGASRHPDYADRYVLAGAYWPPQLVLLD
                     GRTLEPLKLISTRGRTADGGRYHPEPRVSDIAGSYLRPEFIANVKETGRVYLFPYYAT
                     DRLQLVDIGTTAELRAGTFSQDKRYFITPADVNAISVVDTKEQRQVAQIPARVFAGGP
                     GVTYDDPEYGPVWSTSTMVEDEIISIGTDPEGHPNYAWETVRSLKGPGSGSMFMASHP
                     ASTNLWVDSPLNADETVSQSVAVFDKRDLDAGYRSIDVAAIADVGEGPSRVLQPTYDP
                     TGREVWVVVWNPQDLNSAIVVIDDRTLKPLAVIDDPRLITPTRIYSLAALRDAPSEN"
     gene            3640..5196
                     /locus_tag="AC16PP_07220"
     CDS             3640..5196
                     /locus_tag="AC16PP_07220"
                     /inference="COORDINATES: protein motif:HMM:NF025052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside
                     phosphotransferase/ATP-binding protein"
                     /protein_id="HIR6481455.1"
                     /translation="MAQPELVEHLLRPEAYPHPCRAVRLVETHISWVFLTGDYAYKVK
                     KPVLFSFVDFSTARLRKHYCEEELRCNRAFSPELYLDVVEIVRDVEGRIRVGGTGEVV
                     EHAVKMRQFPQSSALDRLLVAGVLEPGGLSRFGLELATLHAGLPRLEAPDDELDRRIL
                     GPVLDNFATLRATRAGGDRSDILQRLESASKDAFDGKRTAFSRRLAKGFVRECHGDLH
                     LRNLVMIDGRVRAFDCLEFNPDLRWIDIASDAAFLIMDCAVRDRVDLAYAFQDGYLAG
                     CGDYVGATLSPFYQAYRSVVRAKVAALEIEGSSKDGKLGAARERLDRHLNWAWERLHG
                     PRGRMVLMCGLSGSGKSYVAERLVPRLPAVRIRSDVLRKVAAGLEPLQRSGSSVDAGL
                     YTPSRSQSVYAEMQAIALALAAAGETVIVDATFLERAARKAFRGAAAAAGVPCVVLHC
                     QASEPTMRSRVAAREREGRDASEAGIAVLEAQQSRFEPPTADEETVVVDTETTAVMAQ
                     IEGALRAAFA"
     gene            complement(5202..5636)
                     /locus_tag="AC16PP_07225"
     CDS             complement(5202..5636)
                     /locus_tag="AC16PP_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6481456.1"
                     /translation="MKEPQLKTAMTPFPYAVELSAPIAQARKLMLEHHVHHLPVTEDH
                     ELKGIITDRDIKLLLGPELGSPDPKELTVEDAYIDDCYAVDMGTPLRQVLTTMAARHI
                     GAAVVTAHGRLAGIFTATDACRVFAELLNAQFGSGSEPPDAA"
     gene            complement(5655..5984)
                     /locus_tag="AC16PP_07230"
     CDS             complement(5655..5984)
                     /locus_tag="AC16PP_07230"
                     /inference="COORDINATES: protein motif:HMM:NF045308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR6481457.1"
                     /translation="MNTQEAKSRLEARRAELTDRQERVARHTRHRDEPLSQDFAEQAV
                     ELENGETLVALDREISQELVQIDHALKRIDADTYLICEGCGEPVGEERLKALPYASRC
                     IDCASER"
     gene            6165..6611
                     /locus_tag="AC16PP_07235"
     CDS             6165..6611
                     /locus_tag="AC16PP_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6481458.1"
                     /translation="MPYKHVLVAVDLSEESTQVLDKAREAADGNGAKLSLLSVVKPLT
                     QVYGGLDMAPIASGAVSFEEEALQRAEAQLKDLGSRYGVADDDVHVLLGAPAHEIREA
                     AKAYAADLIIMGTHGRHGLGLLLGSTANAVLHGLECDVLVVRVHFE"
BASE COUNT         1438 a         2353 c         2724 g         1563 t
ORIGIN      
        1 atacttcatg cggttgccac ggacgtgctg accatgcatg gtaccgatta aacgaaagcg
       61 atcgacggga gaacgggttt gaagatcagg tcggccatgg tggtgatggc gaagcccaag
      121 cacaaacagt ttgctctgga gagggccaag cagctgcagc agagttgtgg cgcccggctg
      181 aggctggtct ccttctgctg gaacgccatg tgcgagcagt cggacgtgtt cgaggcgcat
      241 cagcgccgtg cgatcaaagc tacgatcatg cgtgagcgtg agtcttggct gatcgatcag
      301 gtccgcgatg caggtatggc agccgcggac gtcagcatcg aggttcagtg gaccgacaac
      361 atcgcaggct gggtcagcga gaaagcggcg gccaacgagt ttgatctggt gctcaagtcc
      421 gtcaatcgct cccggacgct cgttcacacg ccgctggact ggcagctcct ggagcagtgt
      481 tccaagcctg tctacatatc ggcgccgccg cgccgctcag cgtcgggtaa tgtcctcgcc
      541 gccctggatt tccgtcatcg cgacaggaag catcaggccc tcaacatccg tgttctggag
      601 gcggcccagg agtttgccga catgtcgggc ggcaagctgc actgcgtgaa cgtggtggag
      661 ttttcacagg tgttgagcga ccttgacctg atcgatccgc gcagcgtcaa gaagaaggcg
      721 ctggccaaca accaggagct catgaacgcg ctgctggagc cgttcgatgt gcccaagtcg
      781 aggatgcaca cgccgctggg caaggtcggt cagatgattg cgatcaccgc gcgcaagatc
      841 gatgcggatc tgctcgtgat cggtacgagc acccggcgca agggtggcac cgtcgtcatc
      901 gggaattccg ctcagcgaac gctcgagaaa tcgccgtgcg acttgctggc cgtccacccg
      961 tagccaggta acgatccggc agtattgctc tcatccggtc atgaaacagc cgggaccgag
     1021 gcgtccgcaa aggcatgacg ggtcattcgt cttccggagc gatctggatg gattcgatga
     1081 aattcaccag cgcgtcgatt ttttgctcga cgcggctctg ggcttgctct ccgtagcctt
     1141 ccatgcgggt gaaggcctct ccccagacgg gcatgccgtc tggtccgtgg gcaagcctga
     1201 gctcgcggcc atcgatgatc tccgtgacga accagcgggg aaactcgccg tcattgcgct
     1261 cggccaggta gcgcagatcc tggaggacga ccgacagaga cggcgtcacg atgccgtcgc
     1321 cctcgccgta catgccgtga cagttggcgc agtttgcctg gtagagctcg ccgccgtcgt
     1381 aggcgatgtc gtcggcggca gccagaccga agtagcaggt tgccgccagg gccaggatca
     1441 gcttgagagt gttgagcacg ttcagttctc cgatggagcg tcccgcaagg cggcgagact
     1501 gtagattctg gtgggcgtga tcagccgggg atcgtctatc acagccagcg gcttcagggt
     1561 acggtcatcg atgaccacga tggcggagtt caggtcctgg gggttccaga cgaccaccca
     1621 cacctctcgg ccggtcgggt cgtaggtcgg ctgcaggacg cggcttggtc cctcgccgac
     1681 gtcggcgatg gcggcgacgt cgatgctgcg gtagccggca tccaggtcgc gtttgtcgaa
     1741 cacggcgacg gactggctga cggtctcgtc agcgttgagc ggtgaatcga cccagaggtt
     1801 ggtggatgca ggatggcttg ccatgaacat cgagccggac ccgggtcctt tcagcgatct
     1861 caccgtctcc caggcgtagt tggggtgacc ctcgggatcg gtgccgatgc tgatgatctc
     1921 gtcctcgacc atggtgctcg tggaccagac gggcccgtac tccggatcgt cgtaagtcac
     1981 gccgggcccg ccggcaaaca cccgggcggg tatctgtgcc acctggcgct gttccttggt
     2041 gtctacaacg ctgatggcgt tgacgtcggc cggcgtgatg aagtagcgct tgtcctgaga
     2101 aaacgtgccc gccctgagtt ccgccgtggt gccgatgtcc accagctgca ggcggtcggt
     2161 ggcgtagtag ggaaacaggt agacccggcc cgtctccttc acgttggcga tgaactcggg
     2221 gcgcaggtat gagcctgcga tgtcgctgac tcgtggctcc ggatgatagc ggccgccatc
     2281 ggccgttcgg cctcgggtgg agatcagctt caggggttca agcgtgcggc cgtccaggag
     2341 cacgagctga ggaggccagt aggcgccggc aaggacgtaa cgatccgcgt aatcgggatg
     2401 gcgtgaagcg ccaacggctc tcgcttcgta gccgaccctt acgctggcca ccctgcgcgg
     2461 cttcctggcg tacaggtcga tcatggtgag cgatccgtcc cgggcgatca cgtagagata
     2521 gcggccgctg gcggatccgc gaacccggtg cgggctctgg cccgttgcca ccgtgccgat
     2581 gacgcgtcgc gaaggaccgt ggatcagcgc tatctgagca acatcgtgca gggtcaccac
     2641 gaacagctca tccagcggat ggcgcccctg gggcacccct ggacggtcct ttagaggtac
     2701 ctccaaagac cagctcgcgc gcacgtctgc ctcggtcatg tcgggagcct gaggcaccgg
     2761 gtgctgcaga aagcgagcga gcatgttgat ctcctcggct gtcagcgcgg ccgaggtgcc
     2821 ccagttgaac attcccgcgg atgaaccgta gttgatgacc gcctgaaggt attcggtccc
     2881 gcgttggcgc atcagcaccg gcgtcaatgg cgatcccgca gtaccttcgc ggagcacgcc
     2941 gtgacaaccc gtgcagtgcc cgtagtacag agatcgcgcc cggtcgaact cgctcacgga
     3001 caggggaggg ccgctcgacg tcacgtactg caccgcgtcc atatcggcca gcgggctcac
     3061 atcgggcagt cccgggtggg gggccggttg gcttgccagc agctcgagtc gctcagcggc
     3121 gacctcctcg gcggtgtaag gctgcccccc cggcccccat tcctgcagca ggaacgtcag
     3181 ggtactggcg acggtctcgt tgccgagata gccgtgggtc ggcatgaggg gggaattgac
     3241 cacggtgtcg tcagcgcgtc cggcaagcac cccacgaatc gccgttttcc ggtcgagcca
     3301 aagggaggga gcatttctca ggctcggata gagcccgtct acgccgcccc cgtcgcgctg
     3361 gtggcatcgg gcgcactgct gctcgtagag ctccttgccc gtcttttcct gttctgccgt
     3421 attggggtgg gctaccatcg gagagatgag cgggcagacg gcaaacaagc cgatcaacgt
     3481 ggccttaatg ggtttagctg gcgtcaagag ggtttcccgg catggcatgg tcgtgtcaat
     3541 gctaacggtt ttcggcgacg atttgcctat ccgtagttcc cctagtgagc atctggcggc
     3601 aggctcggac gtcagccccg ctccggcgag gtgttgatag tggcccagcc cgagctcgtc
     3661 gagcatctgc tacgtcccga ggcttacccg cacccctgta gagccgttcg cctggtcgag
     3721 acgcacatct cctgggtctt cctcacggga gattacgcct acaaagtcaa gaaaccggtc
     3781 ctcttcagct ttgtggattt cagcaccgct cgtctcagga aacactattg cgaggaggaa
     3841 ctccgctgca accgggcgtt ctctccggag ctctatctgg acgtggtcga gatcgtccgg
     3901 gatgtcgagg gccggatccg ggttggcggg actggcgagg tcgttgagca tgcagtgaag
     3961 atgcggcagt ttcctcagag cagcgcgctg gatcggcttc tggtcgcagg cgttctcgag
     4021 cccggtgggt tgagtcgatt cggactggag ctggccactc tgcatgctgg gctgccacgt
     4081 ctggaggccc cggacgacga gctggaccgg cggatcctgg ggccggtgct ggacaacttc
     4141 gcgacgctca gggcaacccg ggccggcggg gatcgctcgg acatcctcca aaggctcgag
     4201 tcggcctcga aggacgcttt tgacggcaag cgaacagcgt tctcgcgccg cctggccaag
     4261 ggcttcgtgc gagagtgtca cggcgatctg cacctgcgta atctcgtcat gatcgacggg
     4321 cgggtgaggg cgttcgactg tctcgagttc aaccccgacc tgcgctggat cgacatcgca
     4381 agcgacgccg ctttcctgat catggactgt gcggtgcgag atcgcgttga cctggcctac
     4441 gcttttcagg acggctacct ggccggctgc ggagattacg tcggggcgac gttgtcccct
     4501 ttttatcagg cctaccgatc cgtcgtccga gccaaggtgg ctgccctgga gatcgaaggg
     4561 tcgtcgaagg acggcaagct gggcgctgcc cgggaacgtc tcgaccggca cctgaactgg
     4621 gcctgggagc gtctgcatgg gccccggggc aggatggttt tgatgtgcgg tctatccggc
     4681 tctggaaaat cgtatgtagc ggagcggctc gtgccgcgat tgcccgctgt gcgtatccga
     4741 tctgacgtgc tccgcaaggt ggcagccggc ctggaaccgc tgcaacgatc cggatcatcg
     4801 gtggatgcgg ggctgtacac gccttcacgg agccaatcgg tttacgccga gatgcaggca
     4861 atcgcgctgg ctttagctgc tgcaggcgaa acggtcatcg tggacgccac ttttctcgag
     4921 cgcgcggcaa gaaaggcttt ccgcggcgcc gcggcggcgg cgggcgtgcc ctgcgttgtc
     4981 ctgcattgcc aggcatccga acccacgatg aggtcacggg tggcagcccg ggagcgggag
     5041 ggcagggatg cctccgaggc cggcatcgcg gtgctagagg cgcagcagtc gcgatttgag
     5101 ccgcccacag cggatgagga aacggtcgtg gtcgacacgg aaaccacagc cgtgatggcg
     5161 caaatcgagg gggcccttcg ggcggcgttt gcctgagcct atcaggcagc gtccggtggt
     5221 tcgctgccgg acccgaactg ggcgttgaga agctccgcga aaacgcggca cgcatcggtg
     5281 gcggtgaaga ttccggcaag tcgcccgtga gcggtgacca cggccgcgcc gatgtgccgc
     5341 gcagccatgg tagtcagcac ctgccgcaga ggtgtcccca tgtcgacggc gtagcagtcg
     5401 tcgatgtagg catcctctac ggtaagctcc ttgggatccg gggagccgag ttcggggccg
     5461 agcagaagct tgatgtcccg gtcggtaatg atgcctttga gctcgtgatc ttccgtgacc
     5521 ggaagatgat ggacatggtg ttcgagcatg agctttcggg cctgggcgat cggagccgag
     5581 agctcgacgg cgtaggggaa aggcgtcatg gccgttttga gctgcggttc cttcaccagg
     5641 ctgccggatc cggttcagcg ctcgctggcg cagtcgatgc agcggctggc atagggcaga
     5701 gccttcagtc gctcctcacc gacgggctcc ccgcatccct cgcaaatcag ataggtgtct
     5761 gcatcaattc gtttcagggc gtgatcgatc tgaacgagct cctggctgat ctccctgtcc
     5821 agagcgacga gggtctctcc gttttccagc tccaccgcct gctcggcgaa atcctgcgac
     5881 agcggctcgt cccggtgtct ggtgtggcgg gcaacgcgct cctgccggtc cgtgagttcc
     5941 gcccggcgcg cctccagcct cgatttggct tcttgtgtgt tcactgtatg ctccgagttc
     6001 gtccggcttg aacgtgttcg ggtagatcat accgcctctc gctgcccgcc gtccttacgc
     6061 agtttccgaa tagaggatcg ccgaagttcc cggtaccgac aaggatggct ctcgctgcga
     6121 tggtgggcta atctcaagga gaaatgatca cgggaggtgg cccgatgccg tacaaacacg
     6181 tgctggtggc ggtagatctg tccgaagagt cgacgcaggt actcgacaag gctcgggaag
     6241 ccgcggacgg caacggggct aaactgtctc tcttgagcgt ggtaaagccg ctgactcaag
     6301 tctacggtgg tctggacatg gcccccattg ccagcggcgc ggtttccttc gaggaggaag
     6361 cgctgcagcg agcggaagcc cagttgaagg acctgggcag ccgttacggc gtggcggacg
     6421 atgacgtcca tgtgctgctc ggcgcgcccg cgcacgagat ccgtgaggcg gccaaggctt
     6481 acgccgccga tctcatcatc atgggcactc atggccggca cggcctgggg ctcctgctcg
     6541 gctccacggc aaacgccgtt ctgcacgggc tggaatgcga cgtgctggtc gtgcgcgtgc
     6601 acttcgagtg aagcggacgc gaccgcgcca attgacgttg cgctcgaggc cttgcgtatt
     6661 gaacgagatg catgtgcgga aaaagtcgag ctttcagttg cttggcagcg cgagctcaaa
     6721 cgcgggcaag cttgcgtgaa cttttccaca ggattgcgac ggtctgatcc acaacatatc
     6781 cacacaacct tgcggatgtt tcattggtca gctccgacca gttgggtaac actgtgtcca
     6841 cggtctgcgg tcaaagcccc tgaaaggggg ccgcgaaacg caagaccgga agacccccgc
     6901 cagggtcggg gttgccggag gcgccggcag ctcaagggag agtcccgagc atgggagagc
     6961 cgttggccga tggcttgctc gtgcgaaggc tggctcgctg gaaggagggc gtcccttagg
     7021 ggaagcttac gccggaaacg tggggcagtt gaggggcttg cttccgccct tcgatccgga
     7081 gtgatcgcaa cggatcggag cgacgtagca aggcgtcgtt aaataacgac tctcgagtcg
     7141 tggccgtgga cctcgagtgc cgtcgatggc ggcaggagag taaaggggcc tccggatgag
     7201 aggtgggaac cggccgcaag gcgcgaacat cacgctcacc gggttgccag gcttcggtcg
     7261 ggcaacgttc agggaacccc gcttcggcgg ggttttcttt tttcgcttac cgcgttatgc
     7321 cgccgatggt caatcggtcc atggataaaa gcgaaggtgc ggtgagcgat aagaggcgga
     7381 aaaaacaaag ccgcccggag gcggcctaaa tatccctaga tttttgttgt tgtccgggat
     7441 ccgattgtcc gatttcccct ccccccacga atctgcagcg tcctcatcga cctggcaggg
     7501 cgaaatcgtt gggaaattta acagttcgcc cacctagaat caacaagtta ccggaaggtt
     7561 ctcaggcacg aatcggcaat cgctcgtatc tgcagcgcgt ttggcgtgag gggcagatcg
     7621 gcggctagtt cggtagtcga gcattgctcg acgtgtgcgc gtgggtcggt gcggtggagg
     7681 atctgtcggg gctggagcca gaggctcagg attggttcca aagggagaca cttccagata
     7741 acctgcacga tctcctggcc gaggcgggtc gggtccatgt ccccgttttg acgtcattat
     7801 ccggagcggc gctggcggat cgttggcgtt gggcgcgatg ttgccgacgt cgcttgcggg
     7861 gcgggttgaa gatgccgttc cggtgaaggg aaacttgatt gttgattgat taagcgagga
     7921 aagcagacca tgagagaagt ggtaattgtc gatgcggcga gatcggccgt cgggcgcaag
     7981 aagggcacgt tggggttcgt acacccgacc gacgtcctag gacaggtcct catgaagctc
     8041 gtggaacgaa atggtctcga gccgagccag gttgatca
//
LOCUS       DBNHKV010000228         7004 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_812092, whole genome shotgun sequence.
ACCESSION   DBNHKV010000228 DBNHKV010000000
VERSION     DBNHKV010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7004
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_812092"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(436..840)
                     /locus_tag="AC16PP_07240"
     CDS             complement(436..840)
                     /locus_tag="AC16PP_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6481459.1"
                     /translation="MSNLPTFGAYAAAFEESLQDDDWTRLEQYFSEDASYRPGDGTEA
                     IGREAVLQALQASVNQLERKCDSRDPVGEPELSESGDTITLKFTVKYTKEGLPDLMLS
                     GYETVQFSDGRILKMEDVLDDASAMLEWTSKL"
     gene            957..1496
                     /locus_tag="AC16PP_07245"
     CDS             957..1496
                     /locus_tag="AC16PP_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481460.1"
                     /translation="MQIANVYSFGRLAKEKQMSISELGSLGEFIGSIAVLVTLIYLVF
                     QLRQNTLSMRSQSRYFVLEALNADLRQVQEEGFFEIAARANREDATEQDHVSFAMYVN
                     GWFSHLEMLFLEIRDGALPKEFADTLRWRTAFLFFSNPNMNQIWEEWQGYYTSSYQKY
                     VDDLRRQDVAAIYAQRRHY"
     gene            complement(1506..1940)
                     /locus_tag="AC16PP_07250"
     CDS             complement(1506..1940)
                     /locus_tag="AC16PP_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009686074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6481461.1"
                     /translation="MTRQEHKGRCHCGAVRFEVRLTDGLSTARRCNCSLCRMRGAIAV
                     TANVGDLNILQGADRLTTYQFNTRTAKHYFCSVCGIYTHHQRRSNSVEYGINAACLEG
                     VSPFDFPDVVVYDGVQHPSDLKHATGPKVAGHLRFFAADAAG"
     gene            2041..2574
                     /locus_tag="AC16PP_07255"
     CDS             2041..2574
                     /locus_tag="AC16PP_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481462.1"
                     /translation="MDHQAFAEMLGNYGEFVGAIAVVATLAYLAIQIRQNTRAMQSTA
                     ELEAARHWSEQNIRAALDPDMTRIADVGNRDAAALNDDERRRYIWFLAHHWYMVDGLF
                     KLHQKGQLSDDAWHPFERQIRGSLSLESIQIWLRSGISPLSDSFRQHFGISAPETWSH
                     SEDLARMFDPGGSSPSD"
     gene            complement(3116..3192)
                     /locus_tag="AC16PP_07260"
     tRNA            complement(3116..3192)
                     /locus_tag="AC16PP_07260"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3156..3158),aa:Val,seq:gac)
     gene            3606..4121
                     /gene="infC"
                     /locus_tag="AC16PP_07265"
     CDS             3606..4121
                     /gene="infC"
                     /locus_tag="AC16PP_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR6481463.1"
                     /translation="MERSLLNEEIMARSVRLIGADGGQVGVVSRDEALDEAKRSGLDL
                     VLIAPEADPPVVKVMDYGKHLFELKKTKAAQKKKQKQMQVKEMKFRPGTEEGDYQVKL
                     RNLIRFLEDGDKAKVSLRFRGREVVHPEIGMNLMSRIEKDLEEYGLVEAEPKFEGRQV
                     VMVLGPKKKKK"
     gene            4160..4360
                     /gene="rpmI"
                     /locus_tag="AC16PP_07270"
     CDS             4160..4360
                     /gene="rpmI"
                     /locus_tag="AC16PP_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR6481464.1"
                     /translation="MPKMKTKSGAAKRFRRTANGFKHRKANRNHILTKKAPKRKRQLR
                     GLHDVSAADAPAINRMIPKKAR"
     gene            4370..4735
                     /gene="rplT"
                     /locus_tag="AC16PP_07275"
     CDS             4370..4735
                     /gene="rplT"
                     /locus_tag="AC16PP_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR6481465.1"
                     /translation="MPRARNTVASRTRRKKVLKQAKGYYGARSRSFKVAKQAVIKAGQ
                     YAYRDRRQRKRQFRSLWVVRINAAAREHDLSYSRFIAGLKKAAIEVDRKVLADLAVHE
                     KETFAVLAEQAKQALAEAA"
     gene            4865..5878
                     /gene="pheS"
                     /locus_tag="AC16PP_07280"
     CDS             4865..5878
                     /gene="pheS"
                     /locus_tag="AC16PP_07280"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010209123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR6481466.1"
                     /translation="MNIEELLEQARTGIAEAGDEAALDAVRVRFLGKKGELTVLLKSL
                     GTLDAEERPKAGAAINLAKEQVQEWIAQRRASLGSEQLAQALASESVDVTLPGRGERP
                     GGLHPVTQAMERIEDIFTGAGYSVVPGQEIEDDYHNFEALNIPAHHPARAMHDTFYFG
                     NGLLLRTHTSPSQVHVMENQAPPIRVICPGRVYRRDSDLTHTPMFHQVEGLVIDRGIS
                     FADLKGTVVGFLQRFFEQDLAVRFRPSYFPFTEPSAEVDVQCVHCLGKGCRVCSQTGW
                     LEVMGCGLVHPNVLEMSGVDSEEFSGFAFGMGGDRLAMLLYEVDDLRMFFDNDLRFLR
                     QFA"
     gene            5897..>7004
                     /gene="pheT"
                     /locus_tag="AC16PP_07285"
     CDS             5897..>7004
                     /gene="pheT"
                     /locus_tag="AC16PP_07285"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020300162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR6481467.1"
                     /translation="MKFSEAWLREWADPGISREALLDQLTMAGLEVDGVEPVSKEFAG
                     VVIAEVVSVDAHPNADKLSVCTVDDGTQTFQVVCGAPNVRAGMIGAFARVGAVLPDDF
                     RIKAAKLRGVESQGMLCSAAELGMGDDHSGILDLDCPDRVGDDLRQALALDDVIVDLD
                     LTPNRGDCLSVRGLAREVGVLNNVPVAAGEVEAVAPVLEDTFSVEIQDPAGCPRYLGR
                     VIRGIDPSCASPLWLTEKLRRCGVRAIDPVVDVTNFVMLELGQPMHAFDLGQLRDGIV
                     VRRARDGEQLRLLDGQELALDADTLLITDGSGPVAIAGVMGGERSGIQAATTDVFLEC
                     AYFAPLAVAGTARRYALHTDASHRYERGVDFELQH"
BASE COUNT         1411 a         2002 c         2205 g         1386 t
ORIGIN      
        1 gaagcatttc gacatgaagc tctttccgtt gttcgagccg gccttggact aaactcccgt
       61 catccacaac gggagacgcc caatgggtat tcaggatctc ggggccatcg gggagttcat
      121 cggctcgctg gtcatcattt tcacgctgat ggtgctgatc tacgaagtgc gcggtgcgaa
      181 gaacgcactt caagtctggt ttggcagcct gcctttgaga aatggtatga cttcagtttg
      241 tcccttcagc gtggcaccgg ccttgaccga tttcttgatc acgtggacga gatcagagcg
      301 agtatgcaga cgacatagat gccgacatct cccggcaacg gcattgcacc ggcaccagcg
      361 ggcacccgga ggcatgcgat gcggtttcga gggccgtcgg ctccgccatc tatcgatagg
      421 ctcccctcgt tgccgtcaga gcttgctggt ccactccaac atggccgacg cgtcatccag
      481 cacgtcttcc atcttcagaa tccggccgtc ggagaactgc acggtctcgt accccgagag
      541 catcagatca ggtagaccct ccttggtgta cttgaccgtg aacttcaacg tgatggtgtc
      601 tccggactcg ctgagttcgg gttctccaac cggatccctg gaatcgcatt ttctctccag
      661 ttgattgacg ctggcctgca gcgcctggag aacggcttcg cggccgatcg cctctgtccc
      721 gtcgccgggg cgataggacg catcctccga aaagtactgc tcgagcctgg tccagtcgtc
      781 atcctgcagg gattcttcaa aggctgccgc gtaggcaccg aaggtaggca gattactcat
      841 gttgggggct cctccatgat tgtttgaatg ccaggataca cccggtgcgg tccgatatct
      901 gcgttcccgg gccagctgct ggcgctggtt gtcgagaccg ccgtcgcatt cattggatgc
      961 agatcgccaa cgtgtattct ttcggacgtc tggcaaagga gaagcagatg tcgatttccg
     1021 agttagggtc gcttggggaa ttcattggtt cgatcgccgt gctcgtcacg ctgatctatc
     1081 tcgtctttca actcaggcag aacacgctct cgatgcgctc ccagtcccgt tacttcgtgc
     1141 tcgaggcgtt gaatgcagat ctgaggcagg tacaggagga ggggttcttc gagatagcgg
     1201 cacgggccaa cagggaagat gctacggagc aggaccatgt aagttttgcg atgtacgtca
     1261 atggatggtt cagccacctc gagatgttgt tcttggagat tcgcgacggt gctctgccga
     1321 aggagtttgc ggatacgcta cgctggcgga cggcgttcct gttcttctcc aatccaaata
     1381 tgaaccagat ctgggaagag tggcagggct actacacgag cagttaccag aaatacgtcg
     1441 acgacctccg aaggcaggat gtcgcggcaa tttacgctca gcgccggcac tattgagggc
     1501 ctgcatcagc cagcagcgtc agcggcgaag aacctcaagt gtcccgccac tttgggcccc
     1561 gttgcatgct tcaggtccga tggatgctga acgccgtcgt agaccacgac atccggaaag
     1621 tcgaaggggc tcaccccctc cagacacgcc gcgttgatgc cgtactctac ggagtttgat
     1681 cgacgttggt ggtgcgtgta gatcccgcag accgagcaga agtaatgctt cgctgtccgg
     1741 gtgttgaatt ggtacgtcgt caatcgatcg gccccctgca gaatgttcag atcgccaacg
     1801 tttgccgtca cggcgattgc gccgcgcatc cggcatagag agcagttgca tcggcgcgcc
     1861 gtgctcaaac catcggttaa cctgacctcg aatctgacgg caccgcagtg gcaacgaccc
     1921 ttgtgctctt gtcttgtcac gactcttctc tctttgtgaa cgggcagatg gtagcgcaga
     1981 aggcttgcgg cgcagaccgg ctgaggtagc ctcgttccat tcattggaga ggggacaatc
     2041 atggatcatc aggcttttgc agagatgctt ggtaactacg gggagtttgt cggcgccatc
     2101 gcggtagtag cgaccttggc ctatctcgca attcaaattc ggcagaatac tcgagcgatg
     2161 caaagcacgg ctgagcttga ggccgcgcgc cattggtcgg agcagaatat cagggcggcc
     2221 ctcgatccag atatgacgcg gatcgctgat gtcggcaatc gagatgctgc tgcgttaaac
     2281 gacgatgaac gcaggcgata catctggttc ctcgcgcatc actggtacat ggtcgacggt
     2341 ctcttcaagc ttcaccagaa gggacagctc tcagacgacg catggcatcc tttcgaacgg
     2401 caaatccgag gatcgcttag cttggagtca attcagatat ggttgagaag cgggatttcg
     2461 cctctttccg attcgttccg gcaacatttc ggtatatccg caccggaaac atggagccat
     2521 tcggaagacc tcgctcgaat gtttgatcct ggaggctctt cgccgagcga ttgacctgcg
     2581 ctaggcttcc tcaatcaccc cgattccatt cgcgccggtg ctggacgatc tgtatctgcc
     2641 gggtcaggca gaaaacatgg cggccgtggg cgagaccggg ctgatcggag agctacgcgc
     2701 taccgccggc gggtctcaag ccaatgtttg agggactcca gcgattcgcg ataacccgca
     2761 tcagcggctt gctggaccgc atcgaattgc agcatgcctg ttgatcggcg tggaatattg
     2821 accccctttg cggggtaatc ggcgtccaaa attgaccctc cctgcgaagt gtagatcact
     2881 ccgaggcttt ggctttggag cggcgaaggg agatgttgat cgtggagacg attgggaaga
     2941 tccgccggga ttactttgtt catggcaaga cgatcaaggc ggttgcgcgc gagcgtgggc
     3001 tgtcacgcaa taccgtgcgc aaggtgctga gagcgaggtc acccgaatct gcactccata
     3061 cgcactccac gttatcgagc gattcgggcg aactatccta accaattgat ttaattggta
     3121 ggcgcgattg gaatcgaacc aacgacctct accatgtcaa ggtagcgctc taaccaactg
     3181 agctacgcgc ctagcgtttg cgggaccgga atgtgcccgt tgcgggtcaa cgcctcggct
     3241 ccggacgggc aatcatacaa aaatcggccg ggttgcaaaa ttgaatcggc ggcatggatt
     3301 aactcacctt acccgtcttc ggcgcaccgt ggccgcggcc gcatcagaat tttgctcgcg
     3361 gggacaatca gttagcgcac ttcgggcttg tgcggccggt cgcacaggca ttagaatccg
     3421 ccccctgccg ctcagcggtg ggccctgttt ccgtggagat gggggaaata aatgggaatg
     3481 ggtcgagcgc aaaccccggc gagggcgcgt gtcggcccgt tttcgtagga aaaagcgcat
     3541 tgaggtatta acctcgcgct ggagattgat ggagaacgcc tattaagaga ggtggagcga
     3601 agcgcgtgga gcgatctctc ctcaacgagg agatcatggc tcgcagcgtg cgtcttatcg
     3661 gcgctgacgg cggtcaggtc ggtgtagttt cccgggacga agccctggac gaagccaaac
     3721 ggtcgggtct tgatctggtg ctcatcgcgc cggaggccga cccgccagtc gtcaaggtca
     3781 tggactacgg caagcatctg ttcgagctga aaaagaccaa agcagctcag aagaaaaagc
     3841 agaagcagat gcaggtcaag gaaatgaagt ttcggcccgg aacggaggaa ggcgattatc
     3901 aggtcaaact acgcaacctg attcgattcc tcgaggacgg agacaaagcc aaggtttcgc
     3961 tgaggttccg gggcagagaa gtcgtgcacc cggaaatcgg tatgaacctg atgtcgcgga
     4021 ttgagaagga cctcgaagaa tacgggttgg tggaagccga gccgaagttc gaggggcggc
     4081 aggtggtgat ggtcttgggt cccaaaaaga agaagaaata gaacagcatc atacaagtct
     4141 ggaaattgcg agtaagttca tgccgaaaat gaagacgaaa agcggcgcgg cgaaacggtt
     4201 tcgccgcact gcaaatggtt tcaagcaccg caaggcgaat cggaaccaca tattgaccaa
     4261 gaaggcgccg aagcggaaac gccagcttcg cggccttcac gacgtatctg cggctgatgc
     4321 cccggcgatc aatcgcatga ttcccaaaaa ggcgcgatag gaggacggga tgcccagagc
     4381 aagaaatacc gtcgcctcgc ggacgcgccg gaaaaaggtg ctcaagcagg ccaagggcta
     4441 ttacggagcg cgcagccggt ccttcaaggt cgccaagcag gcggtcatca aggccggaca
     4501 gtacgcctac cgcgatcgcc gtcaacgcaa gcgccagttc cgttcgttgt gggttgtcag
     4561 aatcaacgcc gcggcacgcg agcacgacct ttcctacagc cgatttatcg ccggcctcaa
     4621 gaaggcggca atcgaagtgg atcgaaaggt gctcgccgat ctggccgtac acgagaagga
     4681 aaccttcgcc gtgctggccg agcaggccaa gcaggctctc gcggaagcag cctgaagcac
     4741 tgatcggtaa acatcagccg tcttctgggg gccgtcgaag ggctcttccc ggaagcggac
     4801 gcgtacgagc gaatctaggg aaggagccgg cagcggtact tccctttttt cgtttgagac
     4861 cgagatgaac atagaagagc tactcgagca ggccaggacg ggaatcgccg aagcagggga
     4921 tgaagcggct ctcgacgccg tgcgcgttcg ctttctcggc aagaaggggg aactcaccgt
     4981 tctcctgaag tccctgggta ctctcgatgc cgaggaacga cccaaggctg gcgcggccat
     5041 caatcttgcg aaggaacagg ttcaggagtg gatcgcgcag cgccgcgcgt cgctcggctc
     5101 cgagcagctt gcccaggcgc tcgccagcga aagcgttgac gtgaccctgc ccggtagggg
     5161 agagcggcct ggaggcctgc atccggttac ccaggcgatg gagcggatcg aggacatctt
     5221 caccggagcc ggctacagcg tcgtcccggg ccaggagatc gaggacgact atcacaattt
     5281 cgaggctctc aacattcccg cgcaccatcc ggcccgggcc atgcacgaca ccttctactt
     5341 cggcaacggc ctgctgctga ggacccacac ctcgccgtct caggttcacg tcatggagaa
     5401 ccaggcgccg cccatccggg tcatctgccc ggggcgcgtc tatcgacggg attccgatct
     5461 cacccatacc cccatgtttc accaggtcga agggctggtc atcgatcgcg gcatcagctt
     5521 tgccgatcta aagggcacgg tggtgggctt tctgcagcgg tttttcgagc aggatctcgc
     5581 cgtccggttc cggccttcgt acttcccgtt caccgagccg tctgccgaag tggacgtcca
     5641 atgcgttcac tgcctgggca agggctgccg ggtctgcagc cagaccggct ggctcgaggt
     5701 gatgggctgc gggctcgtcc acccgaacgt gctcgaaatg tcgggcgtgg acagcgagga
     5761 gttctcgggg ttcgccttcg gcatgggcgg cgaccggctc gccatgctgc tctacgaggt
     5821 cgacgacctg cgcatgttct tcgacaacga tctccgcttc ctgcggcagt tcgcgtgacc
     5881 gggtcggggg gcgatcatga agttcagtga agcctggctg cgcgagtggg cggacccagg
     5941 catctccagg gaggcgctgc tggaccagct gaccatggcc gggttggagg tggacggcgt
     6001 tgagcccgta tccaaagagt ttgccggcgt cgtgatagct gaggtggtct cggtggacgc
     6061 ccaccccaat gcggacaagc tttcggtttg caccgtcgac gatggcacac agaccttcca
     6121 ggtggtgtgc ggcgcgccaa acgtccgggc gggcatgatc ggcgccttcg cccgggtggg
     6181 ggccgtgctt ccggatgatt tccgcatcaa ggcggccaag cttcgcggag tcgagtccca
     6241 gggcatgctc tgcagcgcgg ccgagctcgg catgggcgat gaccacagcg gcatcctcga
     6301 cctcgactgc cccgaccgcg tgggcgatga cctgcgacag gcgctggccc tcgacgacgt
     6361 catcgtcgac ctggacctca cgccgaaccg cggtgactgc ctgagcgtgc gaggtctcgc
     6421 ccgggaagtg ggggtgctca acaacgttcc ggtcgccgcc ggcgaggtgg aggccgtcgc
     6481 gcccgtgttg gaggatacgt tcagcgtcga gatccaggat cccgcaggct gcccgcgata
     6541 tctgggacgg gtaattcggg gcattgatcc ctcctgcgcg agcccgctgt ggctcaccga
     6601 gaagctgcgc cgctgcggcg tgcgcgccat cgacccggtg gtggacgtca ccaatttcgt
     6661 catgctcgag ctgggtcagc ccatgcatgc cttcgacctc gggcagcttc gcgacggcat
     6721 cgtggtccgg cgggcgcggg atggcgagca gctgaggttg ctggatggcc aggaactggc
     6781 cctggacgcg gacaccctgc tgatcacaga tggcagcgga ccggtcgcca tcgccggggt
     6841 gatgggcggg gagcgcagcg gcattcaggc ggcaaccacc gacgtgttcc tggagtgcgc
     6901 ctacttcgcc ccgctggcgg tagccggcac ggcgcgtcgg tacgcgcttc acacggacgc
     6961 gtcgcaccgc tatgagcggg gcgtggattt cgagctgcag catg
//
LOCUS       DBNHKV010000229         6729 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1449951, whole genome shotgun sequence.
ACCESSION   DBNHKV010000229 DBNHKV010000000
VERSION     DBNHKV010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1449951"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..884
                     /gene="miaA"
                     /locus_tag="AC16PP_07290"
     CDS             3..884
                     /gene="miaA"
                     /locus_tag="AC16PP_07290"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR6481468.1"
                     /translation="MPASLISMDSALVYRGMDVGTAKPSPESLIEHPQALIDVRDPKE
                     PYSAAEFVADADSLVQEAWAAGRVPVVVGGTMMYLRAFREGLADLPQADPQLRRAIAA
                     EAESRGWEALHEQLRAVDPAAAQVIHPNNPQRLQRALEVHRLTGRPISDFWSEQRNAD
                     FAARLGGRMETIALMPDRRDRLHARIARRFAGMLERGLVEEVRQLRARGDLHADLPSM
                     RAVGYRQVWQHLEGELDAAEMRERACAATRQLAKRQLTWLRGWSWVREVEWGDPVALA
                     EQALLRVDQPHGSARRA"
     gene            996..1244
                     /gene="hfq"
                     /locus_tag="AC16PP_07295"
     CDS             996..1244
                     /gene="hfq"
                     /locus_tag="AC16PP_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HIR6481469.1"
                     /translation="MSKGHSLQDPYLNTLRKERIPVSVYLVNGIKLQGQIESFDQFVV
                     LLRNSVSQMVYKHAISTVVPSRNVRLSGPGGSEEAAEE"
     gene            1299..2648
                     /gene="hflX"
                     /locus_tag="AC16PP_07300"
     CDS             1299..2648
                     /gene="hflX"
                     /locus_tag="AC16PP_07300"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HIR6481470.1"
                     /translation="MFFERPEAGRRSVLLQVHLKSAAAPDAAVLAESELEAIELARSA
                     GVDVCQVVSARRDNPHPKWFVGPGKVAELAEVLASQEADLLIVNNELNAGQQRDLERD
                     LLCRVLTRTELILHIFADRARTYEGQLQVELAQLRHAQTRLVRGWTHLDRQKGGIGLR
                     GAGETQIEMDQRALGEHIKRVQRKLEQVARRRDQNRRRRRRTRTRTVSLVGYTNAGKS
                     TLFNALTRAGVLVEDKLFATLDPTMRRLTVPGAGDVVLSDTVGFINQLPHDLVDAFKA
                     TLEEVSNADLLLHVVDAGCRDIAERIGQVREVLAEIGASDLPTILVFNKIDQLTPAER
                     RAVLAADVWSAGDGNAMAVAVSAVTGEGLDELTEAIGRRLGVAPEPAKVVLPADDGKT
                     RAWLYRMGAVLGEEITEDGRVALRVRADADLLERLGRTPRVLLQVNDAVPRILPLPN"
     gene            2670..3806
                     /gene="hflK"
                     /locus_tag="AC16PP_07305"
     CDS             2670..3806
                     /gene="hflK"
                     /locus_tag="AC16PP_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003380712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HIR6481471.1"
                     /translation="MAWNEPGGGDKDPWGSRGDQGPPDLDEAFKKLQAQLASIFGGGR
                     GGSGGDGSGLNAGMLGIVLVVAAVAYGLFGLYQLDEQERGVVFRFGAAKEDIVLPGLH
                     WNPPIIDVVRKVNVTRVNSKNHQALMLTEDENIVDVSLTVQYVVNDPLKFLVMVRDPE
                     NSLDHAVESALRHVVGSSLMDDVITAGRAAIAAEVQDRLQTYLDTYGTGILVSKVNID
                     ESAPPNQVRDAFNDVQKAKEDEQRVINEANAYAESIIPEARGEAQKQIEQANAYRDQV
                     IARAEGEAERFEKLLTEYSLAKEVTRDRLYIDAMESVLSRSSKIMIDVEGGNNLLYLP
                     LDRLGQTGSPVAGLATDESVRSIADAILREVNERIASGRIRESR"
     gene            3815..4681
                     /gene="hflC"
                     /locus_tag="AC16PP_07310"
     CDS             3815..4681
                     /gene="hflC"
                     /locus_tag="AC16PP_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HIR6481472.1"
                     /translation="MNLKNLIIVVIAIIAVLLLMNSVYTVQETERAILLRFGAVSQAD
                     VPPGLHFKMPIAEEVKKADGRVLTLDSVPETYYTLEKKPLIVDSFAKWRIVDVQRYYT
                     GTSFDERRGQRVLQERVNEGLRNQISRRDMHEVVSGERDQLMQELLGDLNAVMSGAVG
                     IEVIDVRVKRIDLPPDVSSSVYDRMNSERQIEARQYRAQGRELALGIRADAERQTVII
                     EAEAYRSSEQIRGDGDARSANIYASAYNRDPEFYQFYRSINAYAKVFKDKSDILVLDP
                     TSEFFKYLANDS"
     gene            4733..5947
                     /locus_tag="AC16PP_07315"
     CDS             4733..5947
                     /locus_tag="AC16PP_07315"
                     /inference="COORDINATES: protein motif:HMM:NF009086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR6481473.1"
                     /translation="MVNRKWRLPDGVDELLPPEAWALEQVRRQVLDVFNSWGFDYVEP
                     PVVEYLDALLVGAGPDGGLGHQDDLDLQTLKVVDQRSGRLMGVRADMTAQAVRIDAHS
                     RPSEGVQRLCYAGPVVLANPAAALDSRVPLKAGAEVFGAPGLAADVEVICLLLEILER
                     TGVEAPVLVLGHMGIYLSLVAPLGLDAFTERQLFAAVQSKSEADIGELIGERSDGALL
                     ERLPTLMGGREILREARAALAGGPDGAVAAVDALEELAEAVLVRNPAVEVRYDLAELA
                     GYGYHNGPVFSAYQADQGRAIARGGRYDGIGAPFGRSRPATGFDVNLRQLIGLAGAGT
                     VGQAVWAPWTGSLGGDAAAALAAEVRRLRAAGERVVVALDDEDGAPSGCDRQLSYVSG
                     VWKVERLGRNEG"
     gene            5949..>6729
                     /locus_tag="AC16PP_07320"
     CDS             5949..>6729
                     /locus_tag="AC16PP_07320"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HIR6481474.1"
                     /translation="MGRNVVVMGTQWGDEGKGKIVDLLTDQVAAVVRFQGGHNAGHTL
                     VIEGEKTVLHLIPSGILRREVQCFIGNGVVLEPNALLSEVSGLEARGVPVRERLRISP
                     ACPLILPYHVKLDLAREEARGNQKIGTTGRGIGPAYEDKAARRGVRLGDLFYWQEFAA
                     KLAEVMEYHNFMLERYYGSETVDYAQTLDECAAVAEQIRPMVADIIPLLHEIRESGAN
                     LLFEGAQGALLDIDLGTYPFVTSSNTTAGGTAVGSGFGPRYL"
BASE COUNT         1200 a         2049 c         2338 g         1142 t
ORIGIN      
        1 gagtgccggc cagcctgatc agcatggatt ccgccctcgt ttaccgtggc atggacgtcg
       61 gcacggccaa gccttcaccg gagagcttga tcgagcaccc ccaggctctg atcgacgtcc
      121 gtgacccgaa agagccatac tcggctgcgg agttcgtcgc cgatgccgac agcctcgttc
      181 aggaggcctg ggcggcgggt cgcgtgcccg tcgtggtggg cggcaccatg atgtatctgc
      241 gagcatttcg ggaaggtctc gccgatctgc cccaggccga tccgcagctg cgcagggcca
      301 tcgctgccga ggcggagtcc cggggatggg aggctcttca cgagcagctg cgcgccgtgg
      361 atccggccgc ggcgcaggtc attcatccga acaaccccca acgcctgcag cgggcgctgg
      421 aggttcaccg cctcaccggc cggcccatat ccgacttctg gtcggagcag cgcaacgccg
      481 attttgcggc gcgccttggc gggaggatgg agaccattgc cctgatgccc gaccgtcgtg
      541 accggcttca cgctcggatc gcccgtcgat ttgcagggat gctggagcgg gggctggtgg
      601 aagaggtgcg gcaattgcgc gctcgcggcg atcttcacgc ggaccttccg tccatgcggg
      661 cagtgggtta tcgccaggtc tggcagcatc tcgagggcga gctcgacgca gccgagatgc
      721 gcgagcgtgc gtgcgcggcg acgcggcagc tcgccaagcg tcagctcacg tggctgcggg
      781 ggtggtcgtg ggtcagggag gtcgagtggg gcgacccggt tgcgctggcg gagcaagccc
      841 tgctgcgcgt ggaccagccc cacggaagtg ctcgacgcgc ttgaaatatg ggcaggctgt
      901 agccatagtg aatgtggcgg taaaaggacg agcgctgcgt ccccgaaaag tcctgcaaag
      961 ccgtcgattg gggggcgaac ttgaaggagc tgcaaatgtc aaaagggcat tcgttacaag
     1021 acccttatct caacaccctg cgcaaggagc gcattcccgt atcagtttat ctcgtcaacg
     1081 gtatcaagct gcagggccag atcgagtcgt tcgatcagtt cgtggtcctg ctgcggaact
     1141 ctgtgagcca gatggtctac aagcacgcca tctcgaccgt tgtgccttcg cgcaacgttc
     1201 gcctgtcggg ccccggcggc tccgaagagg ctgcggagga atagaccggt tcaggccggc
     1261 tcttcccttg gcggaaggcg ggcctgaaag gagcacactt gttttttgaa cgacccgaag
     1321 caggccgtag atcggtcctg ttgcaggttc acctgaagag cgctgcggcg cccgatgcag
     1381 ccgtccttgc cgaatcggag ctcgaagcca tcgagcttgc ccgttccgct ggagtcgacg
     1441 tctgtcaggt ggtctcggcg cggcgggata accctcaccc gaagtggttc gtcggccccg
     1501 gcaaagtcgc cgagctcgcc gaggtgcttg cctcgcagga agccgatctc cttatcgtca
     1561 acaacgagct gaacgccggc cagcagcgcg atctcgagcg cgatctgctc tgccgggtgc
     1621 tcacgcgtac cgagcttatc ctgcacatct ttgcggatcg tgcccgaacc tacgagggtc
     1681 agctgcaggt ggagctggcg cagctcagac atgcccagac ccgcctcgtc cggggttgga
     1741 cgcatctgga tcgtcagaag ggtggcatcg gcctgcgggg tgcgggcgaa acccagatcg
     1801 agatggacca gcgggcgctg ggggagcaca tcaagcgggt tcagcgcaag ctggagcagg
     1861 tggcacggcg tcgcgatcag aaccggcgcc gtcggcgccg cacccgcacc cgcacggtct
     1921 cgctggtggg atacacgaac gccggcaagt cgaccctgtt caacgcgctc accagagccg
     1981 gggtgctcgt cgaggacaag ctgttcgcca ctctggatcc aaccatgcgg cgactgacgg
     2041 ttcccggcgc gggcgacgtc gtgctgagcg acaccgtcgg cttcatcaat cagctccctc
     2101 acgacctggt cgatgccttc aaggcgacgc tcgaagaggt cagcaatgcc gacctgctgc
     2161 tgcatgtggt ggacgccggg tgcagggaca tcgccgagcg gatcgggcag gtccgggagg
     2221 ttctcgccga gatcggagcg tccgacctgc cgacgatcct ggttttcaac aagatcgatc
     2281 agctcacgcc ggccgagcgc cgggcggtgc tggccgccga tgtctggtcg gccggggacg
     2341 ggaacgccat ggcggtagcg gtcagcgcgg tcactgggga gggcctggac gagctcaccg
     2401 aagccatcgg aagacgcctc ggcgtcgccc cggagcccgc caaggtggtt ctgcccgccg
     2461 atgatggcaa gacgcgcgcc tggctgtatc gaatgggcgc cgtgcttggc gaagagatca
     2521 ccgaggacgg acgggttgcg ctgcgggttc gtgcggacgc cgatctgctg gagcgcctgg
     2581 gccgcacgcc cagggttctg ttgcaggtca atgacgccgt ccctagaatc ttgcccctgc
     2641 ccaattaatc tttaacggag caccacgaga tggcctggaa cgagccgggt ggaggtgaca
     2701 aggacccgtg gggaagccgc ggagaccagg gcccacctga tctggatgaa gctttcaaga
     2761 agctgcaggc ccagcttgcc tccatttttg ggggtgggcg cggcggcagt ggtggcgatg
     2821 gcagcggttt gaatgcgggg atgctgggca ttgtgctcgt cgtcgcggcc gtagcctacg
     2881 gtctgttcgg cctgtatcag ctcgacgagc aggagcgagg ggtggtgttc cggttcgggg
     2941 cggccaagga ggacatcgtc ctcccgggac tgcactggaa tccccccatc atcgacgtgg
     3001 tccgcaaggt caacgtcacc agggtgaact cgaagaatca tcaggccctg atgctgaccg
     3061 aggacgagaa catcgtcgac gtcagcctga ccgttcagta cgtggtcaac gacccgctga
     3121 aatttctggt catggtccgc gaccccgaga acagcctcga tcatgccgtg gaaagcgcgc
     3181 tgcgccacgt cgtgggaagc tccttgatgg acgacgtcat caccgcgggc cgggcggcca
     3241 tcgcggccga ggtccaggac aggcttcaga cctatctgga tacctacggc acgggcattc
     3301 tcgtgagcaa ggtgaacatc gacgagagcg cgccgcccaa ccaggtgcgc gacgcgttca
     3361 acgacgtcca gaaggccaag gaggacgagc agcgcgtcat caacgaagcc aacgcttacg
     3421 cggaaagcat cattcccgag gccaggggtg aagcgcagaa gcagatcgag caggccaacg
     3481 cctaccgcga tcaggtgatt gctcgcgccg agggtgaggc ggagcggttc gagaagctgc
     3541 tcaccgagta cagcctcgcc aaggaagtga cccgcgaccg gctctacatt gatgccatgg
     3601 agtcggtgct cagccgatcg agcaagatca tgatcgacgt cgagggaggc aacaatctcc
     3661 tgtatctgcc tctcgacagg ctcgggcaga ccgggagccc ggtggccggc ctggccacgg
     3721 acgaatcggt cagaagcatc gcagacgcca ttcttcgtga agtcaatgaa cgcatcgcca
     3781 gcgggcggat tcgggaaagc cgataggatc cgacatgaat ctcaagaatc tcattatcgt
     3841 cgtcatagcc atcatcgccg tgctgctgct gatgaacagc gtctacacgg tgcaggagac
     3901 cgaacgcgcc atcctgctcc gcttcggcgc ggtcagccag gcggatgtcc cgcccgggct
     3961 gcacttcaag atgcccatcg ccgaggaggt caaaaaggcc gacggacgcg tgctgacgct
     4021 ggactccgtg ccagaaacct actacacgct ggagaagaag cccctcatcg tcgactcctt
     4081 cgccaagtgg cgcatcgtcg acgtgcagcg ttactacacg ggcacctcgt tcgacgagcg
     4141 gcgcggccag cgagtgctcc aggagcgggt caacgaaggg ctgcgaaacc agatcagccg
     4201 tcgcgacatg cacgaggtgg tctccggcga gcgcgaccag ctcatgcagg agctgctggg
     4261 ggacctcaat gcggtcatga gcggagccgt gggcatcgag gtgatcgacg tgcgggtcaa
     4321 gcgcatcgac ctccccccgg acgtctcgag ctccgtgtac gatcgaatga actcggaacg
     4381 gcagatcgag gcgcgccagt atcgcgctca gggtcgcgag ctcgcgctcg gcattcgtgc
     4441 tgacgctgag cgtcagacgg tgatcatcga agccgaggcc taccggagtt cggagcagat
     4501 acggggtgac ggggacgcgc gctcggctaa catatatgcc agtgcgtaca atcgggatcc
     4561 cgagttctac cagttctatc gcagcatcaa tgcctacgcg aaggtgttca aggacaagag
     4621 cgacatcctg gtgctggatc ccacgagcga gttcttcaag tatctcgcca acgattccta
     4681 agccgggcta aacgttccgg ctgaaatggt ccgctgattg gacgtcagat ccatggtcaa
     4741 ccgaaaatgg cgcttgcccg acggggtgga cgagctactg ccgcctgagg cgtgggcgct
     4801 ggagcaggtg cgccgtcagg tgctcgacgt gttcaacagc tggggcttcg actacgtcga
     4861 gcctccggtg gtcgagtacc tcgatgccct tctcgtcggc gcaggtccgg atggggggct
     4921 cggtcaccag gacgatctcg atctgcagac gctgaaggtg gtcgaccagc gcagcggacg
     4981 cctgatgggc gttcgcgcag acatgacggc ccaggcggtt cggatcgacg cccacagccg
     5041 tccgagcgag ggggtgcaga ggctttgcta cgcgggcccc gtggttctcg ccaacccggc
     5101 ggcggcgctg gattcccggg ttccgctcaa ggcaggcgcc gaggtgttcg gcgcgcccgg
     5161 gctcgcggcc gacgtggaag tgatctgcct gctgctggaa attctcgagc gcacgggcgt
     5221 cgaagccccg gtgctcgtgc tcggacacat ggggatctac ctgagtctgg tcgcaccgct
     5281 gggtctggat gcgttcaccg agcggcagct gttcgctgcc gtgcagagca agtccgaggc
     5341 agacatcggt gagctgatcg gcgagcgctc ggacggggcg cttttagagc ggctgccgac
     5401 cctcatgggc ggccgcgaga tactccggga ggcgcgcgcg gcgttggctg gcgggccgga
     5461 tggcgcggtg gccgcggtgg atgctctgga ggagctggcg gaggcggtgc tggtccgcaa
     5521 cccggcggtg gaagtgcgct acgatctggc ggagctcgcc gggtacggtt atcacaacgg
     5581 gccggtgttc tccgcctacc aggcggatca gggccgcgcg atcgccaggg gcggacgcta
     5641 cgacggaatc ggcgcgccgt tcggccgcag ccggcccgcg accggcttcg acgtgaacct
     5701 gcgccagctc atcgggcttg ccggggcagg gaccgtcggc caggccgtct gggcgccctg
     5761 gaccggcagc ctcggcggcg atgccgctgc cgcgctggct gcggaggtcc ggcgcctgcg
     5821 tgcggctgga gagagggtgg tggtggcgct ggacgacgaa gacggggcgc cttcgggctg
     5881 cgatcgtcag ctttcgtacg tctctggggt atggaaagtg gaaaggctcg ggcggaacga
     5941 gggctgatat gggccgcaac gtagtcgtca tgggcaccca gtggggggac gagggcaaag
     6001 gcaagatcgt cgatctcctc accgatcagg tagcggccgt ggtccgtttc cagggtggtc
     6061 acaacgccgg ccacaccctg gtcatagagg gcgagaagac ggtcctgcac ctgattccct
     6121 cgggcattct gcgtcgcgag gtgcagtgct tcatcggtaa cggcgtcgtc ctggagccga
     6181 acgcgctgct cagcgaggtg tccggtctcg aggcccgtgg cgtgcccgtg cgcgagcggc
     6241 tccggataag ccccgcctgc ccgctcatcc tgccgtacca cgtcaagctc gatctcgccc
     6301 gggaggaagc caggggtaat cagaagatcg gcacgaccgg gcgcggcata ggccctgcct
     6361 acgaggacaa agcggcgcga cgcggcgttc gtcttggcga tcttttctac tggcaggagt
     6421 tcgcggccaa gctggccgag gtcatggagt atcacaactt catgctggag cggtactacg
     6481 gctcggagac cgtggactac gcgcagaccc tggatgagtg cgcggcggtt gccgagcaga
     6541 ttcgtcccat ggtggcggac atcattccgc tgctgcacga gattcgcgaa agcggagcca
     6601 atctgctgtt cgagggagcc cagggcgcgc tcctcgacat tgatctgggc acgtatccgt
     6661 tcgtgacctc ctccaacacg accgcgggcg ggaccgcggt gggcagtggg ttcggacctc
     6721 gatatctgg
//
LOCUS       DBNHKV010000230         7009 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_598766, whole genome shotgun sequence.
ACCESSION   DBNHKV010000230 DBNHKV010000000
VERSION     DBNHKV010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7009
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_598766"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="AC16PP_07325"
     CDS             complement(<1..721)
                     /locus_tag="AC16PP_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012346.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HIR6481475.1"
                     /translation="MSAKQIVFAHDARARLNRGVDTLARAVNATLGPRGRNVLLSKTW
                     GAPTVTKDGVTVAKDIELKDRFEDMGARIVRQAATRTADEAGDGTTTATVIAHSLFHW
                     GRRAIAAGFSPVEIKRGIDLGVRLMTDALDAMAVECEAGDDIRKVATISANGDEAIGE
                     LIAQAMEQVGREGVITVKDGTGIDDQLEVSEGMQFDQGYLSPYFVTSTETYAAELDRP
                     VILLRDKKISSSRELLPLLEAV"
     gene            complement(912..1469)
                     /locus_tag="AC16PP_07330"
     CDS             complement(912..1469)
                     /locus_tag="AC16PP_07330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6481476.1"
                     /translation="MSHESQRRVSLADLSDEALAASARHQLPADTSAYDELVRRHYES
                     VRRLATAQLGAPDEADALAQDVMLRVFSGLKNFRGDAKFSTWLFRIVENLARTRVRQQ
                     IREQDKREALGMAQHEREEDAPDQSDFQRLLAVLSPQERALLALRFVEDMELHEIAAV
                     LGLSLSATKMRYYRTLDKLKDHIPG"
     gene            1695..4019
                     /gene="ptsP"
                     /locus_tag="AC16PP_07335"
     CDS             1695..4019
                     /gene="ptsP"
                     /locus_tag="AC16PP_07335"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF008283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR6481477.1"
                     /translation="MTMPTMGGKRPENRVSLQLLQRILVDASSAHTVEEQVERLVSQV
                     RDAMAAGACSLYQRFDDTLRMVANCGFAPESVGRIALPMGEGLVGRIARDRMSLNLAD
                     ASAHPDFRLFPQSGEEAFPAFLGVPIIHLGAVNGVLIVQDDRPSAFSEDEVSFLVTVA
                     AQLGPILLPLRAPASTSGRRLRGRSGSPGKAVGRVHLVLSEQTVQLVEEPQSAGTEEE
                     IGVLETAVSRTREKIGRAKSQLEAHVAPDVLELFDLYNAMLSGDRLVTAAAGRVREGH
                     SAFSAVRATVDETIAAFEAIEDDYLSARAEDVRHVGNKLLVEILGSAMPSPEHAEGVV
                     LLGGSVGIADVGAFRPEQLAGIVCLQGSSYSHTAVLARALGIPAVVGIGPVDHIVEGM
                     PVIVDGDGGEVIFEPPGVLLRAYEELVASERQLQNELLAQKDLPGVTTDGCRITLLAN
                     TGLMADVRPGKLRGAEGIGLYRSEIPFLMSPVLPTEAEQYATYRELLEIYHPLPVTIR
                     TVDLGGDKQLPYLPTQEENPALGWRGIRFALDNPAILVSQLRAMLRADHGLGNLQIML
                     PMISDVSEVQEARRLANTVAAALTAEGLDIRMPPLGVMVEIPAVVDVLPDLAPEIDFA
                     CVGSNDLAQYLLAVDRGNPRVVGRYDYLHPGVLGCLDVIARRVEDLGIRLSICGEMAA
                     DPVGVVVLLGLGYRALSMNAYSLPRIRALIRGISIREAQALARSALACRNPGSSRVAI
                     AAALAGLGLDHLLAHGGAFESASTPALAGSPSRP"
     gene            complement(3982..4824)
                     /locus_tag="AC16PP_07340"
     CDS             complement(3982..4824)
                     /locus_tag="AC16PP_07340"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6481478.1"
                     /translation="METELLRTFLEVNRTRHFARAASNLHLTQAAVSARVRHLEEIVG
                     ERLFTRTRNNIQLTVAGHRLLGHAEAIVGSWNRALLETGAPASTRALVALGCLPSIWE
                     TYLTDWLSRTYEDLSPILLQIELLNTEALVRRLRENSITIGLLYEPPQADDLIAEQVT
                     EVSVRLVSSSQGLSVSDSLPGYVYVDWGTSFAIAHDTRLGISTPPALRVDTPAIAYRF
                     LTEHGGTAYLPSAMVAGALSAGLLFPVAGGKTFTRAAFIARSATASAVDSIKAVTASL
                     RALV"
     gene            4945..5202
                     /locus_tag="AC16PP_07345"
     CDS             4945..5202
                     /locus_tag="AC16PP_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481479.1"
                     /translation="MTEIFDEDDEEGLSPHILEQVRRLQARIDRRESLRREHVARSRG
                     LRRRLEDWEEQRALRSSVDYLEMEFAERDGDRRTGRAALPR"
     gene            complement(5228..>7009)
                     /locus_tag="AC16PP_07350"
     CDS             complement(5228..>7009)
                     /locus_tag="AC16PP_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6481480.1"
                     /translation="GGTDSGLHGADLTLRKSADSWVKLQVSQSEGLVTDTLRSSDGGF
                     EFAGYDPSSFSDASAGGYRADVSVGLDDFSSRLDGRITLYAQDLDAGYSGPGLTTVGG
                     ARNFGGTFTMPVTRKLEVSAKSDLRKQRQGVETTAHELDLVYDLTQRWDISAGVRHDE
                     LTDRSPVVAPTQDQGQRIDAVMQVGYDSLGRWKAYGFVQDTVSSDGDRPDNARLGLGG
                     QVRVTEKMTIDSEFSTGDLGTGGKLGTTYMHSERTSLYLNYAMDNERTEPMLRAPRGS
                     EGNLVSGIKTRLSDATSVYVESRYRHGDTANGLTYASGVNFTASNSFNIGANTDIGTL
                     KDLSTGAETDRKAAGVRVAFGTERLQLSSGIEYRSDDTEQLDDTTSNRKTWLFRNTLK
                     YQLNESSRLVGKLNHADSTSSLGNFFAGGYTEAVFGYAYRPVRHDRLNTLMKYTYFYN
                     VPTTDQVSLTQTAADFVQKSHIASVDVTYDLTSRWSIGGKYAYRLGEISYDRENPEFF
                     SNTASLYVVRLDWEFRENWEIMAETRLLDMADFQEQRQGYLVAVSRNFGDHFKVGAGY
                     NFTDFSSDLTDLSFDHQGMFLNLTGAF"
BASE COUNT         1128 a         2209 c         2379 g         1293 t
ORIGIN      
        1 cgaccgcctc cagcagtggc agcagttcgc gggagctgga gatcttcttg tcccgcagca
       61 ggatcaccgg ccgatcgagc tcggcagcat aggtctcggt gctggtgacg aagtagggtg
      121 agaggtaacc ctgatcgaac tgcatgccct cagatacttc gagctggtcg tcgatgccgg
      181 tgccgtcctt gacggtaatc acaccctcgc gacccacctg ctccatggcc tgcgcgatga
      241 gctcgccgat ggcctcgtcg ccgttggcag aaatcgtggc caccttgcgt atgtcgtccc
      301 cggcctcgca ctcgacggcc atcgcgtcca gggcatcggt catcaggcgc acgcccaggt
      361 cgatgccgcg cttgatttca acggggctga atcctgccgc aatggcgcga cggccccagt
      421 gaaagagaga gtgagcgatc accgtggccg ttgtggtgcc gtccccggcc tcgtccgcgg
      481 tccgcgttgc cgcctggcgg acgattcgcg cgcccatgtc ctcgaatcgg tccttcagct
      541 cgatgtcctt ggcgacggtg acgccatcct tggtcaccgt cggtgcgccc caggtcttgc
      601 tcagcagcac gttacgtccc cgaggtccca aggtggcgtt gacggcccgg gccaaggtgt
      661 cgacgccccg gttgagccgg gctcttgcgt catgggcaaa aacgatctgc ttggcgctca
      721 tgcggttttc ctcggttgcc ggttgcggcc ggcaaccgag cgcccgcact tcgccgccgc
      781 ctgtcggttg cgcagttcct gtgctatgag gagggactcc tcacgggtaa tcatcgatcg
      841 gatccggccg tgcaaaaaat cttcagatca gactcgcggc cagtcgaaaa agtcacagca
      901 ggcgatgaaa atcagcccgg aatgtggtcc ttgagcttat ccagggtgcg gtagtagcgc
      961 atcttcgtcg cgctcaagct gagtcctagc acggccgcga tctcgtgcag ctccatgtcc
     1021 tccacaaagc gcagcgcgag cagcgcgcgc tcttgcggag aaagcacggc cagcaggcgc
     1081 tgaaagtcgc tctggtcggg agcgtcttcc tcccgctcgt gctgcgccat gcccagcgct
     1141 tcccgcttgt cctgttcccg tatctgctgt cggacccggg tgcgggccag gttctccacg
     1201 atcctgaaca gccaggtgga aaacttcgcg tcgcctcgga agttcttcag gccggagaac
     1261 acccgcagca tgacgtcctg ggcaagggca tcggcctcgt cgggagcgcc gagctgcgcc
     1321 gtggccagcc tgcgcaccga ttcgtagtgc cgcctgacca gttcgtcata ggccgaagtg
     1381 tcggcgggca attggtgacg ggcactcgcc gccagtgcct cgtcggagag gtcggcaagg
     1441 gaaacgcggc gctgtgactc gtggctcacg cgcgcagtct aatgtctatg gagccgtgga
     1501 caaataggcg cccgtgaaag atcgttcgaa actcgaagcc ggcctgagaa cgctttacga
     1561 gcaggccatg ccggaatctc atgggttcgc ggcgcgtgcg ctcagagtgc gcctcgggcg
     1621 attgctgggc gctgccgcca agaggtccga gcaatgccga cgacaaccgg caggcggacc
     1681 gccgcgagcc ggcgatgacg atgccgacga tggggggtaa gcggcccgag aaccgtgttt
     1741 ccctgcagct cctgcagcgg attctcgtcg atgccagctc ggcgcacacc gtggaggagc
     1801 aggtcgagcg gctggtttcg caggtccgtg acgccatggc agcgggcgcc tgcagcctgt
     1861 atcagcgctt tgacgacact ctcaggatgg tagccaactg cgggttcgct cccgagtccg
     1921 tcgggcgtat cgcgctgccg atgggtgagg ggctcgttgg caggatcgcc cgggaccgga
     1981 tgtcgctcaa cctggccgat gcttcggccc atcccgactt tcgtctgttc ccgcagtccg
     2041 gtgaagaggc gtttcccgcc ttcctcgggg tgccgatcat ccatctgggc gccgtcaacg
     2101 gcgtgctcat cgtgcaggac gatcggccct cggcgttcag cgaggacgag gtctctttcc
     2161 tggtgaccgt agccgcccag cttggaccca ttctgctgcc gctgcgcgcg ccggcgtcga
     2221 cgtccgggcg caggctgcgc gggcggtcgg ggtcgcccgg caaggctgtg gggcgcgttc
     2281 acctggtgct cagcgagcag accgtgcagc tggtcgagga accgcaatct gccggcaccg
     2341 aagaggagat cggcgtcctg gaaactgccg tgtcgcggac ccgagagaag atcgggcgcg
     2401 ccaagtccca gctcgaggcc catgtggccc ccgacgtgct ggagctcttc gatctgtaca
     2461 acgccatgct gtccggagac cggctggtga ccgccgcggc ggggcgggtg cgtgaagggc
     2521 acagcgcgtt ctcggcggtt cgcgcgacgg tcgatgagac cattgccgca ttcgaagcca
     2581 tcgaagacga ctatctcagc gcccgtgccg aggacgtgcg ccatgttgga aacaagctgc
     2641 tcgtggagat cctgggcagc gccatgccgt cgcccgagca tgccgagggc gtcgtgctgc
     2701 tcggcggctc ggtggggata gccgacgtgg gcgcgttcag gcccgagcag ctggccggaa
     2761 tcgtctgcct gcagggctct tcgtactctc acacggccgt gcttgcccgg gcgctgggca
     2821 tccccgccgt cgtcgggatc ggtccggttg accacatcgt cgagggcatg cccgtgatcg
     2881 tcgacggcga tggcggcgag gtgatcttcg agcctcctgg cgtgctgctg cgggcctatg
     2941 aggagctcgt cgcctccgaa cgtcagctgc agaacgaact gctggcgcag aaggatctcc
     3001 cgggcgtcac gacggacggg tgccgaatca ctctgctggc gaatacgggg ctcatggccg
     3061 atgtccggcc gggcaagctg cggggcgcgg aaggcatcgg cctgtatcgc tcggaaatcc
     3121 cgttcctgat gagccccgtc ctgcctaccg aggctgagca gtatgcgacc tatcgcgagc
     3181 tgctcgagat ttatcatccg ttgccggtga ccattcgcac cgtggacctc ggtggtgaca
     3241 agcagctgcc ttacctgccg acccaggagg agaaccctgc gctgggatgg cggggcattc
     3301 gcttcgccct cgacaatccg gcaatcttgg tctcccaact gcgcgccatg ctccgcgccg
     3361 atcatggcct tggcaatctg cagatcatgc tcccgatgat cagcgacgta agcgaagtgc
     3421 aagaggcgag acgcctggca aacacggtcg cggcagcgct cacggcggaa ggcctcgaca
     3481 tccgcatgcc tccgctgggc gtcatggtcg aaattcctgc ggtggtcgac gtgctgccgg
     3541 acctggcccc ggagatcgac ttcgcctgtg tcggttccaa cgatctggct cagtacctgc
     3601 tcgccgtcga ccgcggcaac ccccgggtgg tcggccgcta cgactacctt catcccggcg
     3661 tgctcggctg cctcgacgtg atcgcgcggc gcgtcgaaga tctcggcatc cgcttgtcga
     3721 tctgcgggga gatggcggcc gaccccgtcg gggtggtcgt gctgcttggg ctcggctatc
     3781 gggcgctgag catgaacgca tacagccttc cgcgtatcag ggcgctgatt cgaggcatat
     3841 cgatccgtga ggcacaggcg ttggcccgca gtgcgttggc ctgccgtaat cccgggtcga
     3901 gccgcgtggc catcgccgcg gcgctggccg ggctcgggct ggatcatctg ctggcccatg
     3961 ggggggcgtt tgagtcggcg tctacaccag cgctcgcagg ctcgccgtca cggccttgat
     4021 cgaatcgacc gcgctggcgg tcgcgctcct cgcgatgaac gcggccctcg tgaacgtctt
     4081 ccctcctgcg acgggaaaca gcagccctgc ggacagggca ccggcgacca tggccgacgg
     4141 cagatacgcc gttccgccgt gctcggtgag aaagcggtag gcgatagccg gcgtgtcgac
     4201 ccgcagcgcg ggcggggttg aaatccccag gcgcgtatcg tgcgcgatgg cgaaagacgt
     4261 tccccagtcg acgtaaacgt aaccgggcag agagtccgag acgctgagcc cctggctgga
     4321 cgagaccagc cttacgctga cctcggtgac ctgctcggca atcagatcgt ccgcttgggg
     4381 cggttcgtag agaagcccga tggtgatgga gttctctcgc aggcgcctga cgagcgcctc
     4441 ggtgttgagc agttcgatct gcaacaagat cggcgacagg tcctcgtagg tgcggctcag
     4501 ccagtcggtc agatacgttt cccagatgct gggcagacaa cccagcgcca ccagcgcccg
     4561 cgtgctcgcc ggggcgccgg tttcgaggag ggcccggttc caggacccga cgatcgcctc
     4621 ggcatgtccg agcagccggt gcccggcgac ggtcagctgg atgttgtttc tggtgcgggt
     4681 gaacagccgc tcgccgacga tttcctcgag atggcgcacc cgcgcgctca cggccgcctg
     4741 ggtcaggtgc aggttgctgg cggcgcgggc gaagtggcgg gtgcgattga cttccaggaa
     4801 agttctgagc agctcggttt ccatgccttt tgataaacat ctgttatcaa aaagtacaga
     4861 aatatttgat gtatttgcca aatagccggg cgtatatata cgccgacgtt atccggggcg
     4921 ttgcggcccc gcatcgagag cgacatgact gaaattttcg acgaagacga cgaggaaggg
     4981 ttatcgcctc acattctcga gcaggtgcga cggctgcagg cccgcatcga ccggcgggaa
     5041 agcttgaggc gcgagcacgt ggcgcgcagc cggggactgc ggcgccggct ggaagactgg
     5101 gaagagcagc gggcccttcg ctcgagcgtc gactacctgg aaatggaatt cgccgagcgc
     5161 gacggagatc gccgcaccgg ccgagccgcc ttgccacgct gaccggctgg cccgcacgac
     5221 ttcacggcta gaacgcgccg gtgaggttga ggaacatgcc ctgatgatcg aagctcagat
     5281 ccgtcaggtc ggacgagaag tccgtgaagt tgtagccggc gccgaccttg aagtgatcgc
     5341 cgaagttgcg cgagacggca acgagatagc cctggcgctg ctcttgaaaa tcggccatgt
     5401 ccagcaggcg cgtctcggcc atgatttccc agttctcgcg gaactcccag tccagccgga
     5461 ccacgtagag gctggcggtg ttgctgaaga actcggggtt ttcccgatcg tagctgatct
     5521 cgccgaggcg gtatgcgtac ttgccgccga tggaccagcg tgaggtgaga tcgtaggtga
     5581 cgtccacgga cgcgatgtgg ctcttctgga cgaagtccgc cgcggtctgg gtgagcgaga
     5641 cctgatccgt ggtcgggacg ttgtagaagt acgtgtactt catcagcgtg ttgagcctgt
     5701 cgtgacgcac cggtcggtac gcatagccga acacggcctc ggtgtagccg ccggcgaaga
     5761 agttgcccag cgagctcgtg ctgtcggcgt ggttcagctt gcccacgaga cgggagcttt
     5821 cgttcagctg gtacttcagc gtgttcctga acagccaggt cttgcggttg gaggtggtgt
     5881 cgtccagctg ctcggtgtcg tcgctccggt actcgatgcc gctggagagc tgcaggcgct
     5941 cggtgccgaa agcgacccgg acgccggcgg ccttgcggtc ggtctccgct cccgtggaca
     6001 gatccttcag cgtgccgatg tccgtgttcg cgccgatgtt gaagctgttg gacgcggtga
     6061 agttcacgcc ggaggcgtag gtgaggccgt tggccgtatc gccgtgccgg tagcggctct
     6121 ccacgtacac gctcgtggcg tcggacagcc gcgtcttgat ccctgaaacc aggttgccct
     6181 cgctgccccg gggcgcgcgc agcatgggct cggttcgctc gttgtccatg gcgtagttca
     6241 ggtaaaggct ggtccgctcc gaatgcatgt acgtcgtgcc gagctttccg cccgtgccga
     6301 gatcgccggt ggaaaactcg gagtcgatgg tcatcttctc cgtgacgcgc acctggccgc
     6361 cgaggcctag gcgtgcgttg tctggcctgt cgccgtcaga ggaaaccgta tcctgaacga
     6421 aaccgtaggc cttccagcgg ccgagggagt cgtagcccac ctgcatcacg gcgtcgatgc
     6481 gctggccctg atcctgggtg ggcgccacca cgggagagcg gtcggtgagc tcgtcgtgtc
     6541 gcacgccggc gctgatgtcc catcgctgcg tcaggtcgta taccaggtcg agctcgtggg
     6601 ccgtcgtctc cacgccctga cgctgctttc tcagatcgct cttggcgctg acttccagct
     6661 tgcgcgtcac gggcatggtg aaggtgccgc cgaagttgcg ggcgccgcca acggtggtca
     6721 gaccggggcc ggagtagccg gcatccagat cctgggcgta cagggtgatt cggccgtcca
     6781 ggcgggacga gaagtcgtcg aggccaacgc tcacgtcggc ccggtagccg ccggcgctgg
     6841 catcggagaa cgacgagggg tcgtagccgg cgaactcgaa gccgccgtcg ctcgagcgca
     6901 gggtgtcggt gacgagcccc tcgctctgcg acacctgcag cttgacccag gagtccgcgc
     6961 tcttgcgcag ggtgaggtcg gcaccgtgca acccgctgtc cgtgccgcc
//
LOCUS       DBNHKV010000231        17539 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1591756, whole genome shotgun sequence.
ACCESSION   DBNHKV010000231 DBNHKV010000000
VERSION     DBNHKV010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 17539)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17539)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17539
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1591756"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..2623
                     /locus_tag="AC16PP_07355"
     CDS             203..2623
                     /locus_tag="AC16PP_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007978235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcvT family protein"
                     /protein_id="HIR6481481.1"
                     /translation="MTLPERTDVVIIGGGIIGASIAYHLAELGKLDVCLIERGRLTCG
                     TTWHAAGLVAELRASPNLTRLARYSAELYESLESAGEHTGFRRVGALTLACQPERVRE
                     LKRQAAMARQNQVSCNWLEPPELAERWPDLDLSGVLGAVFMPRDGQTSPVDTTRALAN
                     RARQNGARVLEGVSVTRIEVASGAVAGVTTQRGHIACERVIVCAGLWSRALAADAGAI
                     LPIYPAEHFYAVSEPIDAASRLPIVRSPDQGIYLKPDAGRLLVGCFESAAKPLAPESL
                     PDEFAFDELPFDLDHFAPYLQNAVDRLPALGNTGIRTWFNGPESFTPDGRYIIGPLPE
                     IRGLFAACGMNSIGIQSAGGIGQVMARWVTDGRPPMDLWEVDARRFMPFQNETDYLVE
                     RTSEALGLLYAMHWPYRQHESARPIRQSPLHERLAAAGACFGELAGWERANWYARPGQ
                     APRYEYSYGRQNWFPNAAQEHRAAREAVALFDQSSFAKYEVSGPDACRLLNRLGTSDI
                     DVAIGRVVYCQWLNERGGIEADVTVTRLGPETFWVVSAAPCQRRDLAWLAEHSAGFQA
                     DVKDVTDQHAVIGVMGPDSRRLLTSLADADFGHDAFPFATSQPIAIANTALRATRITY
                     VGALGWELYVPRAEAVTVYDAIAGAGPVHAGYHAMDSLRLEKAYRHWGHDITDEDTPI
                     EAGLSFTAAWDKPEGFIGREALLEQRADGVTKRLALFVLNDPDPLLYHDEPIWWNGRP
                     VGRITSGAYGHTLGRSMGFGYVAADAPFPRQALREGRFQIEIEDTLVDAACHLRAPFD
                     PDNREILR"
     gene            complement(2630..3175)
                     /locus_tag="AC16PP_07360"
     CDS             complement(2630..3175)
                     /locus_tag="AC16PP_07360"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR6481482.1"
                     /translation="MRLAETLWFVAFVILSVAVPGLAAGSELEVGDPAPGFTLEGSDG
                     RTYSLSDYVGRQAVVIAWFPKAYTRGCTIECKSLAENGHLIRRFDVSYFMASVDPIED
                     NIGFAEQQKADFPLLSDPTKETARAYGVLSFAGFAKRHTIYIAKDGTIAAIDTDVRPA
                     TSAEDIATRLGELGVAAAPES"
     gene            complement(3216..4262)
                     /locus_tag="AC16PP_07365"
     CDS             complement(3216..4262)
                     /locus_tag="AC16PP_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6481483.1"
                     /translation="MDQEHESLMSHGRFDNPVIAALAGFKDRFREQPLASRLTDADRF
                     DGKRVLITGANSGLGYALAVDVARRGGSVTMACRSEIPEAGQRAIGESGSQNIEMRHC
                     DLSRMETLHGFCRSAAADGLRFDVVILNAATTLPRARPAPCGQDEMFVVNYLANFALV
                     HLLIDHGLLVPDARVQPRIVVISSDSHQGSSAIDYEEFGRYFDYGVRKAIANYSYFKL
                     LLNTFATELSRRLNSGDGIRVQVNVICPGPVNSNIIKAAPWALRLVLRAIFRVIFKSP
                     AEAARPVIYMAASSDFAGRTNDYLHMFSEKPMDSKVYDESAGSRLWRESAELWARIDD
                     KAADALSAIDKPGL"
     gene            4444..5535
                     /locus_tag="AC16PP_07370"
     CDS             4444..5535
                     /locus_tag="AC16PP_07370"
                     /inference="COORDINATES: protein motif:HMM:NF014954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HIR6481484.1"
                     /translation="MAIHPLPPTAADIGPVWLTEVLHGSGGLSAEGRVKEVTWEKLGE
                     GAGMMSDLARLRMTYEGDAGSAPATLVVKQPSAVENNRLVAVKYNTYAREVRYFAELD
                     PVCAAQGPGIHLSEIDPDNNFVILMDDLSDYHIGDQIVGADLPQSEVCVDELAKLHGS
                     FWAAADDLDWLPHIHGSEHAGNMISGAQIGWDTMLKIFGEFVPDAINGERERFLDSIG
                     PLQHRLDTPPITVLHGDFRMDNLFFGQNPEQHPLIMLDWQGPLRGRGIYDVAQLLVQS
                     AQTEVRRSHERQLVERYVQKLTDQGVTGYGFDQAWEDYRVATLYLWAYVVVVSGTLEV
                     GSERAFAWMSKMVQRNATAIEDLDCLSLL"
     gene            5577..6917
                     /locus_tag="AC16PP_07375"
     CDS             5577..6917
                     /locus_tag="AC16PP_07375"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIR6481485.1"
                     /translation="MTAAAAGCAQFGVPPLKRLYQSVPVPEDQPPLIVIPGAFGSKLR
                     RRATGEEIWPRSGFALLFSSYPDVEVTIDADTLDPIADEVEAFDIFRKGMGRDFYGAL
                     LKTLESIGGYQRAHPGTPPAPPARSYYVYPYDWRLDNVAAVEGLHRLIEQVVEDHGDP
                     NLRVDILGHSNGGLMARYYARFGTADMLTSRAAEPDWNGSHRIRRLLMVGTPNLGTMQ
                     PVLSHVRGEEIGLNDIPAEVVATCSGAPQLFPAPELTWLVDARGEDLNLDVYALETWR
                     ELGWSIFSDKARDRATSKHGVGRRGRAHLELLERYLAKHLERGRRFQHALARPARPDE
                     PEPFVFGGDCTPTVARLVLEKRGGVFVGREQPDRVAAPVRGVDYDGLINEPGDGVVTR
                     ASLTGQNATPVAPQLNVAHSVFLCEEHQLLTSNASFQDNLLYNLLTEFPQGAHG"
     gene            6963..7661
                     /locus_tag="AC16PP_07380"
     CDS             6963..7661
                     /locus_tag="AC16PP_07380"
                     /inference="COORDINATES: protein motif:HMM:NF016764.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0016192 - vesicle-mediated transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HIR6481486.1"
                     /translation="MSGVHEPDTAVYTRRREELIDEGSGSLAVTTSIPASMMLRLLIR
                     PRSVFRLLADADPAPLVVFFKFALWLGLLPPLFAYIGASTFGWRVGVVEPLFLPQPTL
                     IAISIAYFCSLLFGFFSASIISRWMAVTYGARDSLGVHLAMMVIVGAPLAIASLMHLY
                     PNALLNAVVFVPTMIWSMYLLYRGLPTILHTTPEQGMLMASSIIGYLLVAAVSLLGLT
                     VFLWVMGIGPALGV"
     gene            7665..9104
                     /gene="ccoN"
                     /locus_tag="AC16PP_07385"
     CDS             7665..9104
                     /gene="ccoN"
                     /locus_tag="AC16PP_07385"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045277 - respiratory chain complex IV
                     [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HIR6481487.1"
                     /translation="MSTTNDQTYSDRVVTTLVLLAIAWSIVGMTAGVWAAAQLVWPTL
                     DFGQSWLSYGKVRTLHTNAVIFGFGVTALMGTAFYSVQRTSHVRLFAPGLAWFTCWAW
                     QITVLIGGLSLLAGYNAGKEYAELEWPLDIAITVAWVCFAVVFFGTIARRRIKPIYVS
                     NWFYGGLIIVIAMLHIVNSLALPVTLTKSYSLYAGAQDAIVQWWYGHNAVGFLLTGGF
                     LGMMYYFLPKQAEQPIWSYRLSIVAFWAFTYSYIWAGPHHLHYNSIPEWVQSLGMVMS
                     LVLLAPSWATMVNGIMTVSGAYEKLRTDPAMKFIVLSLAFYGLATFEGPMMSIKSINV
                     VSHFTEWTIGHVHSGALGWNALITFGTFYFLVPRLMGSKLYSVKLANWHFWLALAGTM
                     LYIVAMWGAGVSQGLLWLNIDDMGELRYSFTDIMAATVPYYVIRLLAGAVFLVGALLM
                     AYNLYRTVSGKRTIEVHAPSADDAAGASA"
     gene            9101..9724
                     /gene="ccoO"
                     /locus_tag="AC16PP_07390"
     CDS             9101..9724
                     /gene="ccoO"
                     /locus_tag="AC16PP_07390"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020800672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HIR6481488.1"
                     /translation="MSGIGLHRKLEENSGLLIFGILFVSAIGGLVQVLPSLFQESLKT
                     PAANTRVYTPMELVGRDIYVREACSTCHSQQVRPLFAEVRRYGPYSRSGEFVYDRPFL
                     WGSKRTGPDLHRVGGKYSDAWHEIHMIDPRAVVPDSIMPAYPWLAERAANADGDIEAK
                     LRGLVTLGHPYTDEDIAGAAASLEGATELDALIAYLQMLGTGMEETP"
     gene            9721..9864
                     /locus_tag="AC16PP_07395"
     CDS             9721..9864
                     /locus_tag="AC16PP_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481489.1"
                     /translation="MSYFLFAGDMLVMAFMTILVVWVSLKSSGDQLERAARIPLEDER
                     HDG"
     gene            9857..10774
                     /gene="ccoP"
                     /locus_tag="AC16PP_07400"
     CDS             9857..10774
                     /gene="ccoP"
                     /locus_tag="AC16PP_07400"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HIR6481490.1"
                     /translation="MADMPSDFWSGWIITLTVASLAGLVWLIFSVYFGKDPDSDHTSP
                     VWDETLSEGANPAPMWWFWMILAALVFSAIYLMLYPGLGSHAGIMAWSQGGALEKSMN
                     RFDAAFAERRQGVVSAELADLHDDVDAMGTARGIFTRNCAACHGPQGLGQANAFPNLR
                     DTAWQWGGTPEAISQSITAGRRGAMVGWLPVLGEDGVEQLVTYVKTLSAGTSDPNAPA
                     ATQFTTYCSACHAADGTGVAMLGGPDLTDDVWLYGGSDDALRTSIAEGRNGIMPAFGD
                     RLDDVQLKLLVAWLTRPPKASARTEGAAR"
     gene            10811..11938
                     /locus_tag="AC16PP_07405"
     CDS             10811..11938
                     /locus_tag="AC16PP_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR6481491.1"
                     /translation="MNETDRRAQIGLVAVIGLILWFGLNPFDFARINNVEWLDPGPGL
                     QFGDSGLAVSEQAFTWPSDTGPAAVTVEMWVRASRVSDTEPRAWLVLMDDSKLTPLGV
                     RQEGGDLVVWDAVTNPDGERWYNDFRVPYAMRPGIEQHIAITSHEDGPLIYIDGSSAG
                     TYAGYPIPLARKGEPFGGRLVVGAGPPWVTPWMGEVLGLALYDRALTATEVEQHANAD
                     PGSGFAELARNSGAVGAYQFDAGGGTSVQDQAGGGHDLKIPEYFRPPGRGLLRSVDPA
                     ERTALWNVGDAILNVLGFAPLGFLISIVIRKHVKPTSLILTATMVGFVVSLGIEWTQS
                     LMITRSSSLQDLVLNTVGSLVGAVAALSSSRFRGRENNTKA"
     gene            complement(11981..12057)
                     /locus_tag="AC16PP_07410"
     tRNA            complement(11981..12057)
                     /locus_tag="AC16PP_07410"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12021..12023),aa:Met,seq:cat)
     gene            12142..12915
                     /locus_tag="AC16PP_07415"
     CDS             12142..12915
                     /locus_tag="AC16PP_07415"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6481492.1"
                     /translation="MDKPRTALITGASAGIGKAFADALAAGGVNLVLVARRADRLQQA
                     AQALREAHGIDVATISADLSDPTAPVRIHEETESRGLVIDYLINNAGVAGPDLLEPDT
                     WQANAGFHQLMMIAPAHLCHLYVPAMRARRWGRIVNVASVAARIPRAGGCNYGPAKAF
                     LVALSEELSLTLKAEGIKVCALCPGFTHTEFHNEGSAAELKANTPAFVWYEAETVVKE
                     GLRALERGRSVYVSGRMYRWMDPFFQSVWTRRLFKIAAS"
     gene            complement(12963..14054)
                     /gene="ychF"
                     /locus_tag="AC16PP_07420"
     CDS             complement(12963..14054)
                     /gene="ychF"
                     /locus_tag="AC16PP_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR6481493.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIDAENFPFCTIDPNTGVV
                     PVPDPRLERVAQLARSAKIVPTSMEFVDIAGLVAGASRGEGLGNQFLAHIRETDAIAH
                     VVRCFEDDNVTHVSGAVSPADDIEIINTELALADLETAERVFDRTRRIANAGDKDAKL
                     LVAVLDKVIGGLNTGRPVRALALGPEELKLLHECHFITAKPVLYVANVAEDGFDNNPL
                     LDGVRAIAAAESSEVVVICNKIEAEIVELEEDERAEFLEDLGIEEPGLNRVIRAGYRL
                     LGLHHYFTAGPKEARAWTVRIGTRAPQAAGVIHTDFEKGFIRAEVIAYDDYVGCGGEQ
                     GAKDTGKWRLEGKDYVVQDGDVILFRFNV"
     gene            complement(14055..14663)
                     /gene="pth"
                     /locus_tag="AC16PP_07425"
     CDS             complement(14055..14663)
                     /gene="pth"
                     /locus_tag="AC16PP_07425"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR6481494.1"
                     /translation="MADVSLIVGLGNPGPRYELTRHNAGAGFVEYLANRFGVVLRDEA
                     RFRGRMGRGEVLGHDVRLLIPTTYMNLSGSSVAAVARFYKLAVDEILVAHDEMAFEPG
                     MLRLRTGGGDNGHNGLRSIISQLGNAAGFPRLRIGVGHPGDRGRVEAYLTTERMPESE
                     RGQMEAAWRMEDAVLAAILDGDLQAAMNALHAPAGAGDEGED"
     gene            complement(14666..15355)
                     /locus_tag="AC16PP_07430"
     CDS             complement(14666..15355)
                     /locus_tag="AC16PP_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HIR6481495.1"
                     /translation="MTQRIEIHAELREDVGKGASRRLRRLADKVPGVIYGGDADPVPL
                     MLSSNELSKAMQLEAFYSQILNVVLDGKGEQAVVRDLQRHPASEKVQHIDFLRIRADR
                     LIQVSVPLHFIDEDKCIGVKQGGGQIVHNMNEVEISCLPGDLPEFIEVYMAQVDVNQS
                     VHLTDLALPEGVSIVALMHGSDRDANVVSVQMPRGGLEEEEAAEAAEAAAEEGGEASD
                     EGAGGEEASED"
     gene            complement(15425..16369)
                     /locus_tag="AC16PP_07435"
     CDS             complement(15425..16369)
                     /locus_tag="AC16PP_07435"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR6481496.1"
                     /translation="MSNLMIFTGSSNPVLSARIAEELRMDLGHADLGRFSDGEVNVEI
                     HENVRGQDVFVIQSTCAPTNDNLMELMIMSDAIRRASAQRVTAVIPYFGYARQDRRAR
                     SARVPISAKVVADMLGSVGIDRMLTVDLHADQIQGFFNMPVDNVYGSPVLIDHMLGEH
                     YENPIIVSPDVGGVVRARAVAKQCNDLDLAIIDKRRPQANDSRVMNVIGDVRGKTCIL
                     VDDIVDTAGTLCTAAEALKEEGAIGVVSYITHAVLSGPAIERIAGSELDRMVVTDTIP
                     LSGSAESCSKIEQLSLARLLAEAIRRVSNEESISAMFR"
     gene            complement(16436..16510)
                     /locus_tag="AC16PP_07440"
     tRNA            complement(16436..16510)
                     /locus_tag="AC16PP_07440"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16476..16478),aa:Gln,seq:ttg)
     gene            complement(16660..17190)
                     /gene="lolB"
                     /locus_tag="AC16PP_07445"
     CDS             complement(16660..17190)
                     /gene="lolB"
                     /locus_tag="AC16PP_07445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HIR6481497.1"
                     /translation="MALCAGCASFRPPVETPADFEVRGKAGVVEGAESFSARFVWRQY
                     GSAFTIDLWGPLGQGRVRLDGDERRLAIVDGKGAVISEGPHAEVMRTHLGWSLPLDVL
                     PSWVLGKPGEGTGAGGRQYDDDGRLVAFEQLDWSVAYARHNQVQTARGSRWLPGRITA
                     VRGDYRVRLSISDWVL"
     gene            17299..>17539
                     /locus_tag="AC16PP_07450"
     CDS             17299..>17539
                     /locus_tag="AC16PP_07450"
                     /inference="COORDINATES: protein motif:HMM:NF017051.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR6481498.1"
                     /translation="MAILAYGINYRTAPIDVRERVAFPEDALPAALEDAMSSIPDVSE
                     IAIISTCNRTELIVSAAPEAEESVARWLASYRPVSM"
BASE COUNT         3075 a         5788 c         5497 g         3179 t
ORIGIN      
        1 tggccggagc ggcggccgcc ctgaccgaag acacttcgta atttgccatt ttctttactg
       61 aatccttact gctaatcgtt cccagggcag caacacaccg cccgaaccaa gtatataagt
      121 acttaccgag cccatttcaa gtttgcctgt tatcagtttg accacgcaac ataaggccgg
      181 tttcatgccc cggaccccga aattgacact acccgagcgc accgacgtgg tgattatcgg
      241 cggcggcatc atcggcgcga gcatcgccta ccacctcgcc gagctgggca agctcgacgt
      301 ctgcctgatc gagcgcggac ggctcacctg cggaacgacc tggcacgctg ccgggctcgt
      361 ggccgagttg cgagcctcac cgaacctgac ccgcctggcc cggtactcgg ccgagctgta
      421 cgaatccctg gagtcggcgg gcgagcatac cggcttccgg cgggtgggtg cgctgaccct
      481 ggcgtgccag ccggagaggg ttcgggagct caagcggcag gcggccatgg cgcgccagaa
      541 tcaggtctcc tgcaactggc tcgagccgcc ggagctggcc gagcgctggc ctgatctgga
      601 tctgtccggc gtgctgggcg ccgtgttcat gccccgtgac ggccagacga gccccgtaga
      661 cacgacccgt gcgctggcga accgggcacg acagaacggc gcgcgcgtgc tggagggggt
      721 ctccgtgacc cgcatcgaag tcgcctcggg agcggtcgcg ggggtaacca cacagcgggg
      781 ccacatcgcc tgcgagcggg tgatcgtctg cgccggtctc tggtccaggg ccctggccgc
      841 cgatgcaggc gccatcctgc cgatttatcc tgcggagcat ttctacgcgg tctccgaacc
      901 tatcgatgcg gccagccggc ttcccatcgt gcgctcgccg gaccagggca tctatctgaa
      961 acccgatgca gggcggctgc tggtgggctg cttcgagtcc gccgccaagc cccttgcgcc
     1021 cgagtctcta ccggacgaat tcgccttcga cgagctgccc ttcgacctcg accacttcgc
     1081 gccgtacctg cagaacgccg tggaccgtct gccggcattg ggcaacacgg gcatacgcac
     1141 ctggttcaac ggcccggaga gcttcacgcc ggacggccgc tacatcatcg gccccctccc
     1201 cgagatccgg ggcctgttcg cagcctgcgg gatgaactcc atcgggatcc agtccgccgg
     1261 cggcatcggc caggtcatgg cacgctgggt caccgacggc cgccctccca tggatctttg
     1321 ggaggtggat gcacgccgtt tcatgccttt tcagaacgag accgactacc tcgtcgagcg
     1381 aacgtccgag gctctgggcc tgctctacgc catgcattgg ccgtatcgcc agcacgaaag
     1441 cgcaagaccc atccgccagt cgccattgca cgagcggctg gccgccgccg gtgcctgttt
     1501 cggcgagctg gccgggtggg aacgggccaa ctggtacgcc cgaccgggcc aggcgccccg
     1561 ctacgagtac agctacggca ggcagaactg gttcccaaat gctgcccagg agcatcgcgc
     1621 ggccagggaa gccgtcgccc ttttcgatca gtcgagcttt gccaagtacg aggtttccgg
     1681 gccggacgcg tgccgcctgc tcaaccgcct cggcacgtcc gacatcgacg tcgccatcgg
     1741 ccgggtcgtg tactgccagt ggctcaacga gcggggcggg atcgaagcgg acgtgaccgt
     1801 gacccggctt ggtcctgaaa cgttctgggt cgtatccgct gcgccctgcc agcgccggga
     1861 cctggcatgg ctagccgagc acagcgccgg tttccaggcc gacgtgaaag acgttaccga
     1921 ccagcacgcc gtcatcggcg tcatgggacc tgacagccgc agactgctga catcgctcgc
     1981 ggacgcggac ttcggccacg acgcctttcc cttcgccacc tcacagccaa tcgccatcgc
     2041 aaacaccgcc ctgcgcgcca cgcgcatcac ctacgtcggc gctctgggct gggagctcta
     2101 cgtgccccgg gccgaagccg tcacggtgta cgacgccatc gccggggcag gccccgttca
     2161 cgccggctat cacgccatgg actcgctgcg gctggagaaa gcgtaccggc actggggtca
     2221 cgacatcacc gacgaggaca cgcccatcga ggcgggactc agcttcaccg ccgcctggga
     2281 caagccggag gggttcatcg gccgcgaagc gctgctcgag cagcgggcgg acggcgtgac
     2341 gaagcggctc gccctgttcg ttctgaacga ccccgacccc ctgctgtacc acgacgaacc
     2401 gatctggtgg aacggcaggc ccgtgggccg tatcacatca ggcgcctacg gccacacgct
     2461 cggacggtcg atgggtttcg gctacgtcgc agccgatgcg cccttccccc gccaggcgct
     2521 gagggaagga cgatttcaga tcgagatcga ggacacgctg gtggacgcag cctgccacct
     2581 cagggcgccc ttcgacccgg acaaccggga aatactgcgc tgagcgggac tagctctcgg
     2641 gcgcggcggc caccccaagc tcacccagcc gggtcgcgat gtcctcggca gacgtggccg
     2701 gacggacgtc ggtatcgatg gcggcgatgg tgccgtcctt cgcgatgtaa atggtgtgac
     2761 gcttcgcgaa cccggcgaag ctgaggacgc cgtaggcccg ggccgtttcc ttggtgggat
     2821 cgcttaagag cggaaagtcg gccttctgct gctcggcaaa accgatgttg tcctcgatgg
     2881 gatcaacgct ggccatgaag tagctgacgt cgaaccggcg tatgaggtga ccgttctcgg
     2941 ccagcgactt gcactcgatg gtgcagccgc gggtataggc tttggggaac caggcgatta
     3001 cgacggcctg tcggccgacg tagtcggaca acgaataggt ccgaccgtcg ctgccctcca
     3061 gcgtgaatcc gggcgcgggg tcacccactt cgagctcact gccggcggcc agcccgggca
     3121 cggcgactga taaaatgacg aacgcaacga accagagagt ctcggctaaa cgcacgacgg
     3181 ccagcctcca cgcaaaccag agagtgcaga gcctatcaca gtccgggctt gtcgatggct
     3241 gacaatgcgt cggctgcttt gtcatcaatc ctggcccaca gctcggcgga ctcgcgccac
     3301 agccgggaac ccgcgctttc gtcgtatacc ttcgaatcca tgggtttctc gctgaacatg
     3361 tgaagatagt cgttggtcct gcccgcgaag tcggagcttg ccgccatgta gatgaccggt
     3421 cgcgcggctt ccgccggcga cttgaagatc acacggaaga tggcacgcag gaccagccgc
     3481 agcgcccagg gcgctgcctt gatgatgttc gagttcacgg gaccgggaca gatgacgttg
     3541 acctgaaccc ggataccgtc gcctgagttg agccggcggc tgagctccgt ggcaaaggtg
     3601 ttgagcagca gcttgaagta gctgtagttg gcgatggcct tgcgaacacc gtagtcgaaa
     3661 tagcgtccga actcttcgta gtcgatggcc gagctgccct ggtggctgtc ggaggagatg
     3721 accacgatgc gaggctggac gcgggcgtcc gggacgagca gcccgtggtc gatcagcaga
     3781 tgcacaagcg caaaattcgc caggtaattc actacaaaca tctcgtcctg gccgcagggt
     3841 gccggccggg ccctcggaag ggtggtggcc gcattgagaa tgaccacgtc gaagcgaagc
     3901 ccgtccgcgg ccgccgaacg gcagaacccg tgcagcgtct ccatcctgct caggtcgcag
     3961 tgacgcatct cgatgttctg cgatccgctc tcgccgatcg cgcgctgacc tgcctcggga
     4021 atctccgagc ggcacgccat ggtcacgctg ccgccgcgcc gggccacgtc gacggccagc
     4081 gcatacccga gacccgaatt tgcgccggtt atcagaaccc gcttgccgtc gaagcgatcg
     4141 gcatcggtca gccgggatgc cagcggctgt tcgcggaagc gatctttgaa gcccgccagc
     4201 gcggcgatca ccggattgtc aaagcggccg tgcgacatca gcgactcgtg ctcctgatcc
     4261 atttcccctc gcccgcaccc gggcttgggg tatgcgggtg aaagactacg tcctgccaac
     4321 cacggcgcaa agctgattcg tggcccgggc cgtcaccaac cgcggcgtcc aaggaatggt
     4381 ttagtccgaa caccctgtta tcctcttcgt ttccaggttt cccgtgcatc caggaggacg
     4441 atgatggcga tacatccatt gcccccaacc gcagccgaca tcggcccggt gtggctcacc
     4501 gaggttctgc acggcagcgg cgggctgtcc gccgaaggcc gcgtcaaaga agtcacctgg
     4561 gaaaagctcg gtgaaggcgc cgggatgatg agcgacctcg cccgcctgag gatgacctat
     4621 gaaggcgatg ccggcagcgc gccggccact ctggtggtca aacagccatc ggcggtcgag
     4681 aacaatcggc tcgtcgcggt caagtacaac acctacgccc gggaagtccg ctacttcgcc
     4741 gagctcgatc ccgtctgcgc ggcacaaggc cccggcatac acctttcgga gatcgatccc
     4801 gacaacaact tcgtgatcct gatggacgat ctttccgact accacatcgg cgatcagatc
     4861 gttggtgcgg atctaccaca gtccgaagtg tgcgttgacg agctggccaa gctgcatggg
     4921 tcgttctggg ccgcggccga cgacctggat tggctgcccc acatccacgg cagcgagcac
     4981 gccggcaaca tgatctccgg ggcgcagatt ggctgggaca ccatgctgaa gatcttcgga
     5041 gaattcgtgc ccgacgccat caacggtgag cgcgagcgtt tcctggatag catcggtccg
     5101 cttcagcatc gcctggacac gccgccgatc accgtcctgc acggcgactt ccggatggac
     5161 aacctctttt tcggccagaa ccccgagcag cacccgctga tcatgctgga ctggcagggt
     5221 ccgctgcgag ggcgcggcat ctacgacgtc gcgcagctgc ttgtgcagag cgctcagacc
     5281 gaggtgcgcc gttcccacga acgacagctg gtggaacgct acgtgcagaa actgaccgac
     5341 caaggcgtga ccggctacgg cttcgatcag gcctgggaag actaccgggt agcaaccctt
     5401 tacctgtggg catacgtggt cgtcgtcagc ggcaccctgg aggttggcag cgaacgcgcc
     5461 ttcgcctgga tgtcgaagat ggttcagcgc aacgcaacgg ccatcgagga tctggattgc
     5521 ctgagcctgt tgtagttcca catggttggc cgcgccgcgc ctttctgtcg gcggcgatga
     5581 ccgccgcggc cgccggctgc gcgcagttcg gcgtgcctcc gctgaagcgg ctctatcaat
     5641 cggtgccggt gcccgaggat cagccgccgc tcatcgtgat tcccggtgcc ttcggctcca
     5701 agctgcgccg gcgggccacc ggcgaggaga tctggccgcg ctcgggcttc gcgctgctgt
     5761 tcagcagcta ccccgacgtg gaggtcacca tcgatgcgga caccctggat ccgatcgccg
     5821 acgaggtaga ggccttcgac atcttccgca aggggatggg cagggatttc tacggcgcgc
     5881 tgctgaaaac gttggaatcg atcggcggtt accagcgggc gcatccgggt acgccaccgg
     5941 cgccgcccgc gcggtcttac tacgtgtacc cctacgactg gcggctggac aacgttgctg
     6001 ctgtagaggg actccaccgg ctgatcgagc aggtggtcga ggaccacggc gacccgaatc
     6061 ttcgcgtcga catcctcggc cactcgaacg gcggtctgat ggctcgctac tacgctcgct
     6121 tcggcacggc agacatgctc accagtcgcg cggccgagcc cgactggaac ggaagccaca
     6181 ggattcggcg gctgctgatg gtgggcaccc cgaaccttgg caccatgcag ccagtgttgt
     6241 cccacgtcag gggcgaggag atcggcctca atgacattcc cgcagaggtg gtcgccacct
     6301 gctccggcgc cccgcagctc tttcccgctc cggagctgac ctggctggtg gatgcccgcg
     6361 gcgaggacct gaacctggac gtctatgcgc tggagacctg gcgcgagctt gggtggtcca
     6421 ttttcagcga caaagccaga gatcgggcga cgagcaagca cggcgtgggc cgccggggcc
     6481 gggcacatct cgagctgctc gagcgctacc tggccaaaca cctcgagcgc ggaaggcgat
     6541 tccagcacgc tctggcacga ccggcacggc ccgacgagcc ggaaccattc gtcttcggcg
     6601 gcgactgcac gccaacggtg gcgcggctgg tgctggaaaa gcgcggcggc gtattcgtcg
     6661 gacgcgagca accggaccgg gtggcggctc ccgtgcgcgg tgtggactac gacggtctca
     6721 tcaacgaacc cggcgacggc gtggtgacgc gcgcctcgct gacgggccag aacgcaacgc
     6781 ccgtcgcccc ccagctgaac gtcgcgcact cggtgtttct gtgcgaggag caccagcttc
     6841 tcaccagcaa cgcaagcttt caggacaacc tgctctacaa tctcctcacc gagttccccc
     6901 agggcgcaca cgggtgattc cgggacgtcg ccacgtcctg gccgcatccc ggcaatcgtg
     6961 gattgtccgg agttcatgaa cccgacacgg ccgtctatac tcgccgtcgc gaagagttga
     7021 tcgacgaggg gagcgggtca ctggccgtca caacctccat tccggcctcc atgatgctgc
     7081 gcctgctgat ccgacccaga tcggttttcc gcctgctcgc agatgcggat cccgcgccgc
     7141 tggtcgtgtt cttcaagttc gcactctggc tcggcctgct tccgccactc ttcgcttaca
     7201 tcggcgcgtc cacgttcggc tggcgggtcg gcgtggtgga gccgctgttt ctgccgcagc
     7261 cgacgctgat cgcgatcagc atcgcctatt tctgctcgct gctcttcggc ttcttcagcg
     7321 cgtcgatcat cagtcgctgg atggcggtga cctacggcgc gcgggacagc ctgggcgtgc
     7381 atctggccat gatggtcatc gtcggcgcac cgctggccat cgccagcctg atgcatctgt
     7441 atcccaacgc cctgctgaac gccgtcgtgt tcgtcccgac gatgatctgg agcatgtacc
     7501 tgctctacag aggactgccg acgatcctgc acaccacccc cgagcagggg atgctcatgg
     7561 cctcatcgat catcggctac ctgctggttg ccgccgtcag cctgctcggg ctcaccgtgt
     7621 tcttgtgggt gatgggcatc ggtcccgccc ttggagtctg atatttgagc accacgaacg
     7681 atcagaccta cagcgaccgg gtcgtcacca cgctcgttct gctcgccatc gcttggtcga
     7741 tcgtcgggat gaccgccggc gtatgggcgg ccgcgcagct cgtgtggccg acactggact
     7801 tcggacagag ctggttgtcg tacggcaagg tgcgcaccct gcacaccaat gccgtcatct
     7861 tcggtttcgg cgtgacggcg ctgatgggca ctgcgttcta cagtgttcag cgcacgtccc
     7921 acgttcgtct ctttgcaccg ggtctggcgt ggttcacctg ctgggcgtgg cagatcaccg
     7981 tgttgatcgg cggcctgtcg ctgctggcgg gctacaacgc cggtaaggag tacgccgagc
     8041 tggagtggcc tttggacatc gcgatcaccg tagcgtgggt gtgcttcgcg gtggtcttct
     8101 tcggcaccat cgcgcgtcgc aggatcaagc ccatttacgt ctcgaactgg ttttacggcg
     8161 gcctgatcat cgtcatcgcc atgctgcaca tcgtgaacag cctggccctg ccggtcaccc
     8221 tgaccaagtc ctactctctc tacgccggcg cccaggatgc catcgtgcag tggtggtacg
     8281 gccacaacgc cgtgggtttc ctgctgaccg gcgggttcct cggcatgatg tattacttcc
     8341 tgcccaagca ggcagagcag ccgatctgga gctaccggct gtccatcgtg gccttctggg
     8401 cgttcacgta ttcgtacata tgggcgggcc ctcatcacct tcactacaac tccatccccg
     8461 agtgggtgca gtccctgggc atggtgatga gcctggtgct gctggccccg tcctgggcga
     8521 ccatggtcaa cggcatcatg accgtcagcg gcgcctacga gaagctgcgc accgatcccg
     8581 ccatgaagtt catcgtgctc tccctggcgt tctacggcct ggccaccttc gagggcccga
     8641 tgatgtccat caagagcatc aacgtggtca gtcacttcac ggagtggacc atcgggcacg
     8701 tccactcggg cgctctgggc tggaacgccc tgatcacttt cggcaccttc tactttctgg
     8761 tgccccggtt gatgggcagc aagctctaca gcgtcaagct ggccaactgg cacttctggc
     8821 tggcgctggc aggcaccatg ctctacatcg tcgccatgtg gggcgccggc gtctcccagg
     8881 gcctgctgtg gctgaacatc gatgacatgg gcgagctgcg ctacagcttc accgacatca
     8941 tggcggccac cgtgccctac tacgtcatcc gccttctggc cggggccgta ttcctcgtcg
     9001 gggcgctgct gatggcctac aacctttacc ggaccgtctc cggcaagcgc acgatcgagg
     9061 tgcacgcgcc ctcggccgac gacgccgcag gagcatccgc atgagcggta tcggtctgca
     9121 tcgcaagctg gaggagaact cgggtctgct cattttcggg attctgttcg tgtctgcgat
     9181 cggcgggctg gttcaggtac tgccgagcct ttttcaggaa tccctcaaaa cgcccgcggc
     9241 aaatacccgc gtctacacgc ccatggagct ggtcgggcgg gacatttacg tgcgcgaagc
     9301 ctgcagcacg tgccactccc agcaggtgcg gccgctcttt gccgaggtcc gccgctacgg
     9361 gccctacagc cgctcgggcg agttcgtcta cgaccgcccg tttctctggg gttccaagcg
     9421 cacggggccc gatcttcacc gggtcggtgg caagtacagc gacgcctggc acgagatcca
     9481 catgatcgat ccccgcgccg tggtgcccga ctccatcatg cccgcctacc catggctcgc
     9541 ggaacgggca gcgaacgcgg acggcgacat cgaggcgaag cttcgcggcc tggtgacgct
     9601 ggggcaccct tacaccgatg aggatatcgc cggcgcggcg gcgagcctcg agggcgcgac
     9661 cgagctcgac gcgctgatcg cctacctgca gatgctcggg accgggatgg aggagacgcc
     9721 atgagttact ttctgttcgc cggcgacatg ttggtcatgg cgttcatgac catcctcgtt
     9781 gtctgggtat cgctgaagag ttccggcgat cagctggaac gcgccgcccg aataccgctg
     9841 gaggacgagc gtcacgatgg ctgacatgcc ctcggatttc tggagcggat ggatcatcac
     9901 gctgaccgtg gcgagcctgg cggggctcgt ctggctcatc ttcagcgtct atttcggcaa
     9961 ggatcccgat tcggaccaca cctcgccggt atgggacgaa acgctttcgg agggcgccaa
    10021 cccggcaccg atgtggtggt tctggatgat tctcgccgcc ctggtctttt ccgccattta
    10081 cctgatgctg taccccggcc tcggcagcca cgccgggatc atggcatggt cgcagggtgg
    10141 cgctctggag aagagcatga accgcttcga tgcggccttc gccgagcggc gccagggcgt
    10201 cgtgagcgcc gagctggcgg atctgcatga cgacgtcgat gccatgggca cggcccgggg
    10261 catcttcacc cgcaactgcg cggcctgcca cggtccccag ggactcggcc aggccaatgc
    10321 ctttcccaac ctccgggaca cagcctggca atgggggggc acgcccgagg cgatcagcca
    10381 gtccatcacg gccggccgcc gcggcgccat ggtcggctgg ctacccgtgc tcggcgagga
    10441 cggcgtcgag cagctggtga cttacgtcaa aaccctttca gccggcacga gcgatccgaa
    10501 cgcgccggcg gcaacgcagt tcacgaccta ctgcagcgcc tgtcacgcag ccgacggaac
    10561 cggcgtcgcc atgctcggcg gcccggacct caccgacgac gtgtggctgt acggcggctc
    10621 ggacgacgcg ctgcggacgt ccatcgccga gggccgcaac ggcatcatgc ccgccttcgg
    10681 cgatcgcctg gacgacgttc agctgaagct gctcgtcgcc tggttgaccc gaccgcctaa
    10741 ggcctcggca cgcacggaag gcgccgcccg ctgatccgaa tcgccagatg ctccaaggaa
    10801 tcacccgagc atgaatgaga cagaccgtcg agcgcagatc gggctcgttg cggtcatcgg
    10861 gctgattctc tggttcggcc tgaatccctt cgacttcgct cgaatcaaca acgtcgagtg
    10921 gctcgatcca gggcccggcc tgcaattcgg ggactcggga ctcgctgtct ccgagcaagc
    10981 cttcacgtgg ccgtcggata ccggtcccgc ggcggtgacc gttgaaatgt gggtgcgcgc
    11041 aagccgcgtt tcggacaccg aaccaagagc ctggctggtc ctgatggacg actccaagct
    11101 caccccgctg ggcgtacgtc aggaaggggg agaccttgtt gtctgggacg cggtgacgaa
    11161 tccggatggc gaacgctggt acaacgattt ccgggttcct tacgccatgc gtccgggtat
    11221 cgagcagcac atcgccatca cctcccacga agacgggcca ctcatctaca tcgatggttc
    11281 gagtgcaggc acctacgcgg gctaccccat tccgctagcg cgcaaaggcg aaccgttcgg
    11341 cggtcggctc gtggttggcg ccgggccacc ctgggtcacg ccgtggatgg gagaagtgct
    11401 cgggctcgcc ctatacgatc gcgcgctgac cgcgacggag gtagagcagc atgcgaacgc
    11461 ggacccggga tccggattcg ccgaactggc aaggaactcg ggtgccgttg gcgcctatca
    11521 gttcgacgcg ggcggcggca cctcggttca ggatcaggcg ggcggcgggc acgacctcaa
    11581 gatccccgag tactttcgcc caccgggccg cgggctgctt cggagcgtcg atccggctga
    11641 gcgcacagca ctctggaacg tcggcgacgc gattctgaac gttctgggct ttgccccatt
    11701 gggcttcttg atatcgattg tgatacgcaa gcacgtgaaa ccgacttccc tcattctgac
    11761 cgcaacgatg gttggtttcg tcgtcagcct aggcatcgag tggacacaat cgctgatgat
    11821 tacgaggtct tcatctttac aggatctcgt gctgaacacc gttggcagcc tggtcggagc
    11881 cgtggcggcc ctttccagtt cccgttttcg aggccgcgaa aacaatacca aagcgtaaca
    11941 gagccagatg acacggccgg ttgttcgtcg agaaagggat tggtagctac ggttggactt
    12001 gaaccaacga ccccagcatt atgaatgctg tgctctaacc agctgagcta cgtagccatg
    12061 gcttgcctgt gctggcgaag gatcgcgatt gtcctgacaa gccgatgagc tgtcaacgtg
    12121 cggcggacgc aggaggacgg cgtggacaaa cccagaacgg cgctgattac cggagcatct
    12181 gcagggatcg gcaaggcctt cgccgatgcc ctggcagcgg ggggtgtcaa tctggtgttg
    12241 gtggcgcgac gagccgatcg gctgcagcag gcagcccagg cgctgcgcga agctcacggg
    12301 atcgacgtcg ccacgatcag cgcagaccta tccgatccga cggcccccgt gcgcatacac
    12361 gaggagacgg agagccgtgg cctggtcatc gactacctca tcaacaacgc gggggtagcc
    12421 gggcccgacc tcctcgagcc ggatacctgg caggccaacg ccggcttcca tcagctgatg
    12481 atgatcgcgc ccgcgcacct gtgccatctg tacgtgccgg ccatgcgtgc ccggcggtgg
    12541 gggaggatcg tcaacgttgc atcggtggcg gcgcgcatac ctcgcgccgg cgggtgcaac
    12601 tacggaccgg caaaggcctt tctggtggcg ctctcagagg agctctccct caccctgaag
    12661 gctgagggca tcaaggtgtg cgcgctgtgc ccgggcttca cgcacaccga gtttcacaac
    12721 gaaggatccg cagcagagct caaggcgaac acgcccgcct tcgtctggta cgaggcggag
    12781 acggtggtga aggaaggatt acgggccctc gagcggggcc gcagcgtcta cgtgtccggt
    12841 cgcatgtatc gctggatgga cccgttcttt cagtcggtgt ggacccgccg actcttcaag
    12901 atcgcggcga gctgaacgcc cgacccggcc ggtgcctcga atcctgcgag ccgaccccga
    12961 gatcagacat tgaagcggaa gaggatcacg tcgccgtcct gaacgacgta atccttgccc
    13021 tctagccgcc actttccggt gtccttggca ccctgctcgc cgccgcagcc gacgtagtcg
    13081 tcgtaagcga tgacctccgc gcggatgaat cccttctcga agtcggtatg aatcacacct
    13141 gccgcctggg gcgcccgcgt gccgatgcgg acggtccagg cgcgagcctc cttgggtccg
    13201 gcggtgaagt aatggtgcag cccgagcagg cgatagcctg cccggatgac acgattgagt
    13261 cccggctctt cgataccgag atcctccaga aactccgctc gctcgtcttc ttcgagctcg
    13321 acaatctcgg cctcgatctt gttgcagatg accaccacct ccgaggattc ggcagcggcg
    13381 atggcccgca cgccgtccag caacgggttg ttgtcgaagc cgtcctccgc cacgtttgcc
    13441 acgtagagaa cgggcttggc cgttatgaag tggcactcgt gcagtaactt cagctcctca
    13501 ggaccgagcg ccagcgcccg caccgggcgt cccgtattca ggcccccgat gactttgtcc
    13561 agcacggcga ccagcaactt ggcgtccttg tcgcccgcgt tggcgatgcg ccgggtccgg
    13621 tcgaagacgc gctccgccgt ctccagatcg gcaagggcga gctccgtatt gatgatctcg
    13681 atgtcgtcag caggcgagac cgcgccggag acgtgggtga cgttgtcgtc ctcgaagcaa
    13741 cgcacgacgt gagcaatggc atcggtttct cgaatgtgcg cgaggaactg atttccgagc
    13801 ccttcaccac gggacgcacc ggcgacaaga ccggcgatat cgacgaactc catgctggtt
    13861 ggaacgatct tggccgaacg ggccagctgg gcaacccgct cgagccgcgg atcagggacg
    13921 gggaccacac cagtattggg atcgatggtg cagaacggaa agttctccgc atcgatgccc
    13981 gccttggtca gggcgttgaa cagcgtggat ttgccgacgt tcggcaaccc gacgattccg
    14041 cacttgaatc ccattcagtc ctccccttcg tcgcccgccc ccgccggcgc atgcagggcg
    14101 ttcatggcag cctgcagatc cccgtcgaga atcgccgcca gcaccgcgtc ttccatacgc
    14161 caggcggctt ccatctgacc ccgttccgac tcgggcatgc gctcggtagt gaggtaggcc
    14221 tcgacacggc ccctgtctcc gggatggcct accccaatac gtagacgggg aaaacccgca
    14281 gcgttgccga gctgagagat gatgctgcgc aaaccgttgt gcccattgtc accaccgccc
    14341 gtcctcagac gcagcatgcc cggctcgaac gccatctcgt cgtgggcaac cagaatttcg
    14401 tccacggcca gcttgtagaa gcgcgccacc gcagccaccg agctgccact cagattcatg
    14461 taggtggtgg gaatgagcag gcgcacatca tggccgagga cctcaccgcg gcccattcgg
    14521 cccctgaaac gcgcctcgtc ccgaagcacg acgccgaacc ggttagccag gtactcgacg
    14581 aatccggcac ccgcattgtg gcgcgtgagc tcgtaccgcg ggcccgggtt tcccaggccc
    14641 acgatcaggc tgacgtccgc catggtcagt cttcgctggc ttcctcgccg ccagcgcctt
    14701 cgtcggaggc ttcgccgccc tcttcagcgg ccgcttccgc ggcctcggcg gcctcttcct
    14761 cttccagccc gccgcgcggc atctggacgc tgaccacgtt ggcatcacgg tcgctgccgt
    14821 gcatcaaggc gacgatgctg accccttccg gaagcgccag atcggtgagg tgtaccgact
    14881 gattgacgtc cacctgagcc atgtagacct cgatgaactc gggcagatct ccaggcagac
    14941 agctgatctc gacctcgttc atgttatgca cgatctgacc gccgccctgc ttcacgccga
    15001 tgcacttgtc ctcgtcgatg aagtggagcg gcacggacac ctgaatgaga cggtcggcgc
    15061 ggattcgcag aaagtcgatg tgctgcacct tttccgaggc cggatggcgt tgcaggtccc
    15121 gtaccaccgc ctgctcaccc tttccgtcca gaacgacgtt caggatctgc gagtaaaagg
    15181 cctcgagctg catggccttg gaaagctcgt tgctgctcag catcagcggg accggatcag
    15241 catcaccccc atagattacg ccgggcactt tgtctgcaag gcgacgcagg cggcggctcg
    15301 cacctttccc cacgtcctcg cgcagctcgg catgaatttc aattcgctgc gtcatcagat
    15361 ttctccatca aatacggacg tcccggatcg cgaccaagtc ctggcgtcca attgcaacca
    15421 tcagctagcg aaacatcgcg ctgatggatt cctcgttact cacccgtcgg atggcttccg
    15481 ccagaagacg ggcgaggctg agctgctcga tcttcgagca gctttcggcc gatccgctca
    15541 acggtatcgt gtcagtcacg accatccggt cgagctcgga gccggcgatt ctttcgatgg
    15601 cgggtcccga aagaaccgca tgcgtaatgt aggaaaccac gccgatggcg ccctcttcct
    15661 tgagcgcttc ggccgccgtg cacagcgtgc ccgccgtatc gacgatgtcg tcaaccagga
    15721 tgcacgtctt gccgcggacg tcgccgatga cgttcatcac gcgggaatcg ttcgcctgcg
    15781 ggcgacgttt gtcgatgatg gcgaggtcca gatcgttgca ctgcttggcc accgcgcgag
    15841 cgcgcaccac cccgcccacg tcgggtgaga cgatgatggg attctcgtag tgctcaccga
    15901 gcatatggtc gatcaggacc ggggacccgt aaacgttgtc caccggcatg ttgaaaaagc
    15961 cctggatctg gtccgcgtgg agatcgacgg tgagcatacg gtcgatgccg acgctgccga
    16021 gcatgtcggc gaccaccttg gcgctgatgg gcactcgtgc cgagcgggct cgcctgtcct
    16081 gacgcgcata gccaaaatac ggtatcaccg ccgtcacgcg ctgcgccgat gcgcggcgga
    16141 tggcgtcgga catgatcatg agctccatga ggttgtcatt ggtcggagcg caggtggatt
    16201 ggatgacgaa gacgtcctga ccgcgcacgt tctcgtggat ctccacattc acctcaccgt
    16261 cgctgaacct ccccaggtcg gcgtgcccga gatccatccg cagctcctcg gcgatgcggg
    16321 cgctcagcac ggggttcgag ctccccgtga agatcatcag gtttgacaat tggcttcccc
    16381 tcgtccgcgc tctttgcgcg tgctatgcgg ttgcggcttc aagagatcaa cagtttggct
    16441 ggggtggcag gattcgaacc tgcgaatgcc gggatcaaaa cccggtgcct taccacttgg
    16501 cgacacccca acagacagct cgttgccatg tgcgcccgcc ggcgctgttc ctgacgtttc
    16561 gcggttctcg ccccggcagc ggggcgctat tgtgatgttg ggtctgttgg ctgtcaaacc
    16621 gccagacctg acgggcagac gtccacgtat cggggcccgt cagagcaccc agtcggatat
    16681 ggaaagccgc acccgatagt cgccccgcac agccgtgatc cggcccggca gccagcggga
    16741 accacgagcc gtctgcacct ggttgtgccg ggcgtaggcg actgaccagt cgagctgctc
    16801 gaaagcgacc aggcgcccat catcgtcata ctgccggccg ccggcgccgg ttccctctcc
    16861 cggcttgccc agcacccagc tgggcagcac gtccagtgga aggctccagc caagatgcgt
    16921 acgcatgacc tcggcgtgag gcccctcgga gatcacggct cccttgccgt ctacaatggc
    16981 caggcggcgc tcgtccccat cgagcctgac gcgcccctgc ccgaggggcc cccagaggtc
    17041 gatggtgaat gccgagccgt actgcctcca gacgaagcgc gccgaaaaac tctccgcgcc
    17101 ttcgacgacg ccggccttgc cgcgcacctc gaagtcggcc ggggtctcga cgggagggcg
    17161 gaacgacgcg cagccggcgc agagggccaa acaaaacaac tttaaaatga atggcttaag
    17221 tacgttcaca aatctccgaa gctgctagaa tgctgggtca gcaagaaggc tctcagctga
    17281 gatcataccc tgcacaacat ggcgatattg gcatacggca ttaactatcg cacggctccc
    17341 atcgacgtcc gggagcgcgt cgcatttccg gaggacgccc tgccggcggc cctagaggat
    17401 gccatgtcgt ctattcccga tgtcagcgag atcgccatca tctcaacgtg caaccgcacg
    17461 gagctcatcg tctccgcagc gcccgaggcc gaggaatccg tggcccgttg gctggcgagc
    17521 tatcggccag tgagcatgg
//
LOCUS       DBNHKV010000232         4297 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_740893, whole genome shotgun sequence.
ACCESSION   DBNHKV010000232 DBNHKV010000000
VERSION     DBNHKV010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_740893"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="AC16PP_07455"
     CDS             complement(<1..1072)
                     /locus_tag="AC16PP_07455"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6481499.1"
                     /translation="MRTARFSVCMGARMNEGDRAGGSTARGAGVAFGLVLALLGSQAS
                     ASAIEEIVVSARAVEQSVRDVPVAITAFNEEVMDNMALTSFDDIAAYTPSVDILRISS
                     GNGTSVSIRGISSSAGSLGIEQSVAIVIDGVYYPQGRSINEGLFDTSQVAILKGPQAL
                     YFGKNATAGVISVMTNDPGDELEILGKIGYETELKTTRAEGVLSFPINDRWGVRLALQ
                     GTDMSDGWLENTAQDDVYVTFEGSQDNPAPKDAENPGEESFYGRVTLKGELSDAVTLR
                     LKGSYSDFEYNTMTLGEQYECSALNGVPHITLNGDPDPVTGWQRPTPLGLGECEANRA
                     RGLNPIPPAVAATSKDLSRFGGE"
     gene            complement(1179..2405)
                     /locus_tag="AC16PP_07460"
     CDS             complement(1179..2405)
                     /locus_tag="AC16PP_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6481500.1"
                     /translation="MEKSEFFADARRDLTGPIHGIRVLEITTSWAGPMAGCMLADLGA
                     EVIKVEHPDGEIGRVLGPHLPGGAHLSLLYETANRNKRNLSVDMRKEVGRDLLIDLGR
                     TCDLVVENFKPGTLHGWGLGYEHFCAAKPDIVYVSISGFGQFGPHQARAAYDPLVQAQ
                     SGWMSLNGEIGGGPVKAPTFLGDDLAGMQAALAALAALRHRDATGEGQHVDVALMDAI
                     LGASNMFPSMARLGIEAPRTANQFPQVAPFNVYACRGGSVFLGMSLNSHWEIFANAVG
                     RPELVEDPRYATTELRTQNRESVDELVSRWCSEQDQMRVVELLGGAGLPVAPVNDYRA
                     ATDDPNIEARDMLTDIELSDGSRAPLLGPVPKFSRTPIGVRSGAPTIGQDNASILDEL
                     GIDDARRRALREAGVI"
     gene            complement(2438..3796)
                     /locus_tag="AC16PP_07465"
     CDS             complement(2438..3796)
                     /locus_tag="AC16PP_07465"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6481501.1"
                     /translation="MKPVRNKPSYREIIQGDATPVADVLALTANPQQSTEDIPFEHYI
                     SDEFFEREMRKMWPKVWQIACREEHIPEVGDYFVYDIGRYSILVVRSAEDQIKAYHNS
                     CLHRGTKLKPSGTAGFSEKLQCPFHGWTYQLDGTLEEVPCAWEFPHLDYEANRLPEVL
                     AESWNGFVFINMDQDAQPLLQYLEVLPEHFASWRFDDWYVASHVRKELHCNWKVAMEG
                     FMEAYHTPVVHPEMCQVVGDWNMQHDIFGDHVSRDLCPMGVSSPSLDEPLSEFELLRR
                     ARPGNPLTSETGSAELPDGKTARMVMAEETRRSFRDQFGMDLSDLSDAEVIDSLKYNV
                     FPNLMFSGGAAIHGLSIFRPLGYDTNKCTIDRLTFLPVPKDGRRPPPAEVITIAEGES
                     YATADTLNEFTAHVLDQDTSIMRLQQEGMHASAKGAETLSTYQESRVRWLHETLEKYL
                     SR"
     gene            3913..>4297
                     /locus_tag="AC16PP_07470"
     CDS             3913..>4297
                     /locus_tag="AC16PP_07470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6481502.1"
                     /translation="MADETDTQATNYDDVRQYRLDPEREQELVNTAGECVFCWANKAG
                     HPIGVITAYVPVKGKIWMTATRERVRIKAIARDGRSSVVISSKGTPMGGGKTVTYKGH
                     TVIHDDQDTKMWMYNLLAQGMAASKD"
BASE COUNT          782 a         1408 c         1343 g          764 t
ORIGIN      
        1 gctccccgcc gaagcggctg aggtccttgg acgtcgccgc gactgccggc ggtatgggat
       61 tcagcccacg cgcccgattc gcctcgcact ctcccaggcc cagcggcgtc ggccgctgcc
      121 agccggtgac cgggtcaggg tcgccattca gggtgatgtg gggaacgcca ttgagcgcag
      181 agcactcgta ctgttccccc agggtcatgg tgttgtactc gaaatccgag tacgacccct
      241 tgagcctcag ggtcaccgcg tcgctgagct cgcccttgag ggtcacgcgg ccgtagaacg
      301 actcttcccc ggggttctcc gcgtctttgg gcgcaggatt atcctgactg ccctcgaacg
      361 taacgtaaac gtcgtcctgc gccgtgttct cgagccagcc gtcggacatg tcggtgccct
      421 gcagcgccag acgcacaccc catcggtcgt tgatggggaa agacaggacc ccctcggcgc
      481 gggtggtctt gagctccgtc tcgtagccga tcttgcccag gatctccagc tcgtcgccgg
      541 gatcgttggt catcaccgag atcaccccgg cagtggcgtt cttgccgaaa tacagcgcct
      601 ggggcccttt gagaatcgcc acctggctgg tgtcgaagag gccttcgttg atggagcggc
      661 cctgggggta gtagaccccg tcgatgacga tcgcaacgga ctgctcgatg cccaggctcc
      721 cggcactgga ggatatcccc cggatgctga cggacgttcc gttgccactc gatatgcgaa
      781 ggatgtccac cgatggggta tatgccgcga tgtcgtcgaa gctggtcagc gccatgttgt
      841 ccatgacctc ctcgttgaac gccgtaatgg ccaccggcac gtcccgaacg gactgctcca
      901 cggcccgggc ggaaaccacg atctcctcga tggcgctggc agacgcctgg ctaccgagca
      961 gcgccaggac caggccgaaa gcgacgccgg ccccacgtgc ggtcgacccg ccagctctat
     1021 cgccctcgtt catgcgcgca cccatgcaca cagagaatct cgccgttctc atatctaccc
     1081 tccccaggga tggattcaac tcttttgatt atgtgcgcct tccggacctg caggcaacca
     1141 ccccgacgct tcggctcacg cagcagcgac ggccccgttc atatcacccc ggcctcccgc
     1201 aacgcccggc gccgcgcgtc gtcgatgccg agctcatcga gaattgaggc gttgtcctgc
     1261 ccgatggtcg gcgcgcccga gcgcacgccg attggcgtac gggagaactt cggcaccggc
     1321 ccgagcaacg gagcccggct gccatccgag agctcgatgt cggtcagcat gtcgcgggct
     1381 tcgatgttgg gatcgtccgt cgcggcgcgg tagtcgttga ccggcgccac gggcaggccc
     1441 gcgccgccga gcagctccac cacgcgcatc tggtcctgct cgctgcacca ccgcgaaaca
     1501 agctcatcga cgctctcccg attctgcgtg cgcagctcgg tggtcgcgta acggggatcc
     1561 tcgaccagct ccgggcgccc cacggcattg gcaaagatct cccagtggga gttcagcgac
     1621 atgccgagaa agaccgagcc gccgcggcag gcgtagacgt tgaagggcgc tacctgggga
     1681 aactggttcg ccgtgcgggg cgcttcgatg ccgagccgcg ccatggacgg gaacatgttg
     1741 gaggcgccga gaatggcgtc catcagcgca acgtccacgt gctggccctc accggtggcg
     1801 tcccgatgtc gaagcgcagc cagcgcggcc agcgctgcct gcatcccggc caggtcatcg
     1861 cccaggaagg tgggcgcctt gaccgggccg ccgccgatct ctccgttcag ggacatccac
     1921 cccgattgcg cctgcaccag cgggtcgtag gcggcccgtg cctggtgtgg cccgaactga
     1981 ccgaacccgc tgatggaaac gtagacgatg tcgggctttg ccgcgcagaa gtgctcgtaa
     2041 cccaggcccc aaccgtgcag ggttccgggc ttgaagttct ccaccaccag atcgcacgtc
     2101 cggccgaggt cgatgagcag gtcgcgacca acctccttgc gcatgtcaac gctcaggttg
     2161 cgtttgttgc ggttggcggt ctcgtaaaga agagaaaggt gagcgcctcc cggcagatgc
     2221 gggcccagaa cccggccgat ctcgccgtcc gggtgctcca ccttgatgac ctcggcaccc
     2281 agatcggcga gcatgcagcc ggccatcggc ccggcccagg atgtggtgat ctcgagcacg
     2341 cggataccgt ggatgggacc ggtcaggtcg cggcgagcgt ccgcaaagaa ctccgacttc
     2401 tccaaactag cccccttgcg ctgctgcggc tgcgccgcta ccgggaaaga tacttctcca
     2461 gcgtctcgtg gagccagcga acgcgggatt cctgataggt cgagagcgtc tccgccccct
     2521 tggccgaagc gtgcatgccc tcctgctgca gccgcatgat gctggtgtcc tggtcgagga
     2581 cgtgggcggt gaactcgttc agggtgtccg ccgtggcata tgactcgccc tcggcaatgg
     2641 tgatcacctc cgccggcggc ggccgccgac cgtccttcgg gacgggcaga aacgtcaagc
     2701 ggtcgatggt gcacttgttg gtgtcgtagc cgagggggcg aaatatgctg agcccgtgga
     2761 tggccgcacc gccggagaac atcagattgg gaaagacgtt gtattttaga gaatcgatca
     2821 cctccgcatc gctgagatcg gagaggtcca taccgaactg atccctgaaa ctgcgtcggg
     2881 tctcctcggc catcaccatc cgggccgtct tgccgtccgg aagctcggct gatccggtct
     2941 ccgacgtcag cgggttgccc ggccgcgcgc gccttagcag ctcgaactcg ctcaacggct
     3001 cgtcgagaga cgggctcgag acgcccatgg gacacagatc gcggctgacg tggtctccga
     3061 atatgtcgtg ctgcatgttc cagtcgccga ccacctggca catctcgggg tgcaccacgg
     3121 gcgtgtggta ggcctccatg aagccttcca tggcgacctt ccagttgcag tgaagctcct
     3181 ttcggacgtg gctcgccacg taccagtcat cgaagcgcca gctcgcgaaa tgctcgggca
     3241 gcacctcgag atactgaagc aatggctgcg cgtcctggtc catgttgatg aacacgaagc
     3301 cgttccagga ctcagccagc acctccggca accggttggc ctcgtaatcc agatgaggaa
     3361 attcccaggc gcagggcacc tcctcgagag tcccgtcgag ctgataggtc cagccgtgaa
     3421 acgggcactg aagcttctcc gagaatccag cagtgcccga gggcttgagc ttggtgccgc
     3481 gatgcaggca ggagttgtgg tacgccttga tctgatcttc cgcgctgcgc accaccagga
     3541 tcgagtagcg gccgatgtcg tagacgaagt aatcgcccac ctcggggatg tgctcctcgc
     3601 ggcaggcgat ctgccagact ttgggccaca tcttgcgcat ctcgcgttcg aagaactcgt
     3661 ctgagatgta atgctcgaac ggaatgtctt ctgtgctctg ctgcgggttc gccgtgagcg
     3721 cgagaacgtc cgcaacaggc gtggcatcgc cttgaatgat ctcccggtaa cttggcttgt
     3781 tacgcacagg cttcatgatg tcctccccat acggcccatc gagccgatgt gcaaaccgtt
     3841 atacttgccg gccaaccacg catcaagcgt cgaacacacc ggggcgaagg cacgttgaaa
     3901 gggagcacca cgatggccga tgaaaccgat acccaggcaa ccaactacga cgacgtccgg
     3961 cagtatcggc tggacccgga acgcgagcag gagctggtca acaccgccgg cgagtgcgtc
     4021 ttctgctggg cgaacaaagc gggtcacccc atcggcgtga tcacggccta cgtgcccgtg
     4081 aaaggcaaga tatggatgac ggccacccgc gagcgcgtgc gcatcaaagc catcgccaga
     4141 gacggccggt catcggtggt gatctcgagc aagggcaccc ccatgggtgg cggcaagacg
     4201 gttacctaca aaggccacac ggtcatccac gacgaccagg ataccaagat gtggatgtac
     4261 aacctcttgg cccagggcat ggcagcgtca aaagacg
//
LOCUS       DBNHKV010000233         6356 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1450473, whole genome shotgun sequence.
ACCESSION   DBNHKV010000233 DBNHKV010000000
VERSION     DBNHKV010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1450473"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /gene="hemA"
                     /locus_tag="AC16PP_07475"
     CDS             <1..792
                     /gene="hemA"
                     /locus_tag="AC16PP_07475"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR6481503.1"
                     /translation="TDIGRNPVSVAYAAVTMAQQIFADLHHNKALLIGAGDTIQRVAE
                     HLSALGTAGLAIANRTLANAEALAAGFDAVPMQLTDIAAHLADYDIVITSTGSSLPVL
                     GKGTVEAAIKRRRHKPMFIVDIAVPRDVEPEVAELPDVYLYTIDDLTEIIEENIKQRR
                     SAAESAEHLVMEGAEAYTRERRAHEGQALLRSFRENAEAIQRAELEKALTALGRGGDP
                     NVVLKSLSRSLTNKLIHQPTVAIRDASASDRSDLLEYLKSLYQLD"
     gene            802..1890
                     /gene="prfA"
                     /locus_tag="AC16PP_07480"
     CDS             802..1890
                     /gene="prfA"
                     /locus_tag="AC16PP_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR6481504.1"
                     /translation="MSASMMAKLSDLTDRFDEVSALLSDPETMADRDRFTALSKEYAE
                     LEPLILTFRRHDVLDTELREAQELAGDEDPEMRELAREDVDRLSKALDEEEQELQTLL
                     LPRDPSDASNVYLEIRAGTGGDEAALFAGDLFRMYGKFAESRGWSFEVLGARAGEHGG
                     YKEIITRVAGKDVYSQLKFESGAHRVQRVPETESQGRIHTSACTVAVMPEVDEVDSIE
                     INKSDLREDTFRASGAGGQHVNKTDSAIRLTHLPSGIVVECQDERSQHKNRARALALL
                     QAKLLDAAQSQQRSEQAETRRNLVGSGDRSERIRTYNFPQGRVTDHRINLTLYKLTEV
                     MEGALENVIDPLVREHHADQLRSLGDEQ"
     gene            1887..2687
                     /gene="prmC"
                     /locus_tag="AC16PP_07485"
     CDS             1887..2687
                     /gene="prmC"
                     /locus_tag="AC16PP_07485"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR6481505.1"
                     /translation="MTTVRAWIDRERELDLLDRHLLLAELLQTNRAGVMTYPERVLND
                     AELRTLDLWSSRRRHGEPMAYILGRKDFWGLTLRVNPTVLIPRPETELLVESALEVAA
                     AGQRLLDLGTGSGAVAIALARELANAAVSASDVSQAALKLAAANAAAHGARLTFIASH
                     WFENVTPSYDVIVSNPPYVAERDAHLTSLRAEPTAALVSGADGLDAIRYIVPAARRYL
                     NEAGWLIVEHGHDQGSSVRSLMRCSGYHEVTTRRDLAGLERVTRGRRP"
     gene            2732..3436
                     /locus_tag="AC16PP_07490"
     CDS             2732..3436
                     /locus_tag="AC16PP_07490"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017641082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HIR6481506.1"
                     /translation="MLPEVDVAGQESLLAGTVLIVGMGGLGAPAAIYLASAGVGHLII
                     SDHDRVEASNLQRQIVHHDASIGQTKVASACQSLLQTNPELRITGIDRKLEGDELLKI
                     VGDADVVLDATDNFAARFAINAACWEAGVPLVSGAAIRWEGQLAVFDPRDAASPCYRC
                     LYAEGHDEALNCAENGVIAPLVGVIGTCQAIEAIKLLTGIGEPLTGYVLYFDAKRMDW
                     RKLKLPKDPACPGCSA"
     gene            complement(3402..4049)
                     /gene="lipB"
                     /locus_tag="AC16PP_07495"
     CDS             complement(3402..4049)
                     /gene="lipB"
                     /locus_tag="AC16PP_07495"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR6481507.1"
                     /translation="MADVPVIVRQLGRTRYEPVFRAMQVFTEERAEPTADELWFTEHD
                     PVFTQGQAGKAEHLLAPGDIPVVQTDRGGQVTYHGPGQIVAYLLFDLRRMGLSVRGLV
                     SGIEEAVVRLLADYDIRSAPRRDAPGVYVAGSKIAALGLRVRRGYSYHGLALNVQMDL
                     EPFSRINPCGLFGMEVTQLADLGGPADVQTVEDELLTKLADVYGFTPSSPDTQGP"
     gene            complement(4051..4314)
                     /locus_tag="AC16PP_07500"
     CDS             complement(4051..4314)
                     /locus_tag="AC16PP_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HP0495 family protein"
                     /protein_id="HIR6481508.1"
                     /translation="MTEPPKIEFPCDYPIRVIAENHLALRETVVRIVRLHAPDLAEST
                     VSVRNSREGRYCSVRFSITARGEPQLRALHRALMDEPMVKMVL"
     gene            complement(4307..5494)
                     /locus_tag="AC16PP_07505"
     CDS             complement(4307..5494)
                     /locus_tag="AC16PP_07505"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR6481509.1"
                     /translation="MLRGLLMSFWANHLAAFCLGLAAVAGPAHAGEGPIIPPPPKIAG
                     TSYLLVDADTMKVLVENEIHEPVPPASLTKIMTGYIAAVELEAGRISLEDQVPISVKA
                     WRAPGSRMFVREGTRVGLEDLLRGIIVQSGNDASIAMAEHIAGSEDAFADMMDQQAIQ
                     LGMTTTQYQNATGLPSDDHYTSAWDLAVLTQSLIKRFPDSYKIYSERSFAFNDIEQPN
                     RNRLLWRDRTVDGVKTGHTSAAGYCLVASAKRGNMRLISVVLGTESEEARMRESQKLL
                     SYGFRYFETQKLYDAGVPLKTSKIWYGEADDIQLGLAEEVFVTIPRGHYEDLKAELSV
                     VKTIEAPVAAGQELGELRLHFGDELMLSAPLVALQEVKEADMFSRLLDGTRLLFRDLF
                     GVD"
     gene            complement(5522..6277)
                     /locus_tag="AC16PP_07510"
     CDS             complement(5522..6277)
                     /locus_tag="AC16PP_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HIR6481510.1"
                     /translation="MVVRLRAAGWLLVLTLVACSGPGVRPPELSDGPPAHTPVDLAKL
                     PDPIPREEPKSARGNPPSYTVMGRTYRVMDSAAGYESTGIASWYGRKFHGRATSSGET
                     FDMFELTAAHTSLPIPTYARVTNLHNGRSTIVRINDRGPFHADRLIDLSYAAAVKLGF
                     ATRGTARVRVEALVDSPDYFLQAGAFSDMSSADALKNALGALTGARAYVVRVSSDALF
                     RVRIGPVKSRPEALRLQALIASSDFDRPLILEQ"
BASE COUNT         1169 a         2113 c         2039 g         1035 t
ORIGIN      
        1 accgatatcg gccgcaaccc cgtctcggtg gcctacgctg cggtcaccat ggcgcagcag
       61 atctttgccg acctgcacca caacaaagca ctgctcatcg gcgccggcga cacgattcag
      121 cgggtggccg agcacctttc cgcgctgggc accgcgggcc tggccatcgc gaaccggacc
      181 ctggcgaacg cggaggctct tgcggcaggc tttgatgccg tccccatgca gctgaccgac
      241 atcgccgctc accttgccga ctacgacatc gtcatcacgt ccaccggcag ctcactgccc
      301 gttctcggca aagggaccgt ggaggccgcc atcaagcgtc ggcgccacaa gccgatgttc
      361 atcgtcgaca tcgccgtgcc gcgagacgtg gagcctgagg tggccgagct gcccgacgtg
      421 tacctctaca ccatcgacga tctcaccgag atcatcgagg agaacatcaa acagcgccga
      481 tcggctgccg agagcgccga gcatctggtc atggaaggcg ccgaagccta cacccgcgag
      541 cgtcgcgccc acgagggcca agcgctgctg cgcagtttcc gcgagaacgc cgaagccatt
      601 caacgggcgg agctcgaaaa agcgctgacg gcactgggcc gggggggcga cccgaatgtg
      661 gtgctgaagt cattgagccg gtctctgacc aacaagctga ttcaccagcc gaccgtcgcg
      721 attcgcgatg ccagcgcgag tgaccgcagc gatctcttag aatatctcaa gtctctctac
      781 cagctagact gaatcatgag cctgtccgcc tcgatgatgg ccaagctgtc agatctgacc
      841 gaccgcttcg acgaggtcag cgccctcctg tcggatcccg agacgatggc cgaccgggac
      901 cggtttaccg cgttgtccaa ggagtacgcc gagctggagc ccctcatcct gacgttccgc
      961 cgccacgacg tgctggacac agagctgcgc gaagcccagg agctcgccgg ggatgaggac
     1021 cccgagatgc gcgagctcgc gcgggaggac gtggatcgcc tgtcaaaggc gctcgacgag
     1081 gaggagcagg aactgcagac cctgctgctg ccacgggacc ccagcgacgc gtcgaacgtc
     1141 tacctggaga ttcgcgcggg tacgggtggt gatgaggcgg cgctgtttgc cggggacctc
     1201 ttccgcatgt acggcaagtt cgcggagtcc cgcggctgga gcttcgaagt gctgggcgcc
     1261 cgggcaggcg agcatggcgg atacaaggag atcatcaccc gtgtcgccgg caaggacgtc
     1321 tacagccagc tgaaattcga gtcaggcgcg caccgggtgc agcgcgttcc cgaaacggag
     1381 tcccagggac gcatacacac gtccgcgtgc accgtggccg tgatgccgga ggtggacgag
     1441 gtggactcca tcgagatcaa caagtccgat ctgcgcgagg acaccttccg cgcctccggg
     1501 gccggcggac agcacgtgaa caagacggac tcggccatcc ggctgactca cctgccgagc
     1561 ggcatcgtcg tggagtgtca ggacgagcgc tcgcagcaca agaaccgcgc gcgggcgctg
     1621 gcgctgctcc aggcgaagct gctcgacgcc gcccagtcgc agcaacgatc cgagcaggcg
     1681 gagacgcgcc gtaatctggt gggctcgggc gaccgctcgg agcgcatccg cacctacaac
     1741 tttccccagg gtcgcgtcac cgatcatcgg atcaacctga ccctctacaa actgaccgag
     1801 gtcatggagg gggcactgga aaacgtgatc gaccccctgg tgcgtgagca tcacgcggat
     1861 cagctgcgca gcctgggcga cgagcagtga ccacggtccg tgcctggatc gacagggagc
     1921 gggagctgga tctgctggat cgccacctgc tgctcgccga gctgctgcaa accaaccgcg
     1981 ccggcgtcat gacctatccg gaacgcgttc tgaacgacgc ggaacttcgc accctggacc
     2041 tctggtcgag ccgtcggcgc cacggcgagc ccatggccta catcctcggc cgcaaggatt
     2101 tctgggggct gacgcttcgg gtgaacccga cggtattgat cccccgaccc gagacggagc
     2161 tgctggtgga gagcgcgctg gaggtagccg cggccggtca gcgcctactg gatctcggca
     2221 cgggcagcgg cgccgtcgcc atcgcgctgg cccgggagct cgcgaacgcg gcggtgagcg
     2281 caagcgacgt ctcccaggca gcgctcaagc tcgcggctgc aaatgccgca gcccacggcg
     2341 ccaggctgac gttcattgcg agccactggt tcgagaacgt caccccttcc tacgatgtca
     2401 tcgtcagcaa cccgccttac gttgccgagc gcgatgccca tctcacctcg cttcgggccg
     2461 agcccaccgc cgcgctggtg agcggcgcag acgggctgga cgccattcgc tacatcgttc
     2521 ccgccgccag gcgctacctc aacgaagccg gttggctcat cgtcgagcac ggccacgatc
     2581 agggcagctc ggtccgttcg ctcatgcgtt gcagcggcta ccacgaggtg acgacgcgac
     2641 gggacctcgc cgggctggag cgcgtcacgc gaggccgaag accttgaatg ggctgagcga
     2701 cgatcagctg cttcgataca gccgccagat catgctgccg gaggtcgacg tcgccggcca
     2761 ggaatccctg ctcgccggga ccgtgctcat cgtcggcatg ggtgggctcg gcgcgcccgc
     2821 cgccatttac ctggcaagcg caggcgtggg ccacctcatc atcagcgatc acgatcgggt
     2881 ggaggcttcc aacctgcagc gacagatcgt ccaccacgac gccagcatcg gccagaccaa
     2941 ggtggcatcg gcatgccagt cgctgctgca gaccaacccg gaactgcgga tcaccggcat
     3001 cgaccgaaag ctggaggggg acgagctgct aaagatcgtg ggtgacgccg acgtggtgct
     3061 ggacgccacc gacaactttg ccgcccgctt cgcgatcaac gccgcctgct gggaggccgg
     3121 agtccccctg gtctcaggcg ccgccatccg ctgggaaggt cagctcgccg tcttcgaccc
     3181 gcgggatgca gcgagcccct gttaccgctg cctttacgcc gagggacacg acgaggcgct
     3241 gaactgcgcc gagaacgggg tcatcgcccc tctggtggga gtcatcggca cctgccaggc
     3301 catagaagcc atcaagctgc tcaccggaat cggcgagccg ttgaccggct acgtcctcta
     3361 cttcgacgcc aaacgcatgg attggcgaaa gctgaagctg cctaaggacc ctgcgtgtcc
     3421 gggctgctcg gcgtgaaccc gtagacgtcg gccagcttcg tcaggagctc atcctcgacg
     3481 gtctgcacgt ccgccggacc gcccaggtcg gcaagctgcg tcacctccat gccgaacaac
     3541 ccgcacgggt tgatgcgcga gaacggctct aagtccatct gcacattgag agccagtccg
     3601 tggtaggagt acccgcgccg aactctcagc ccgagagccg cgatcttaga cccggccacg
     3661 tagacccccg gcgcatcacg gcgcggcgcg ctgcggatat cgtagtcggc cagcagcctg
     3721 acgacggctt cctcgatgcc gctgaccagg ccgcgcacgc tcagccccat gcgacgcagg
     3781 tcgaacagca gataggcgac gatctggccc gggccgtgat aggtcacctg cccgccccgg
     3841 tcagtttgca ccaccggaat gtcgcccggc gccaggaggt gctcggcctt gcccgcctga
     3901 ccctgggtga acaccggatc gtgctcggtg aaccagagct catcggcggt gggctccgcc
     3961 cgttcctcgg tgaagacctg catggctcga aataccggct cgtaacgggt ccgccccaac
     4021 tgcctgacga ttacggggac atcagccacc tcacagcacc atcttgacca tgggttcgtc
     4081 catgagagcc cgatgcaggg cccgcagctg gggttccccc ctggcggtta tcgagaagcg
     4141 caccgagcag tagcggcctt cgcggctgtt cctgaccgat acggtgctct ccgccaggtc
     4201 cggggcatgc aggcgaacga tccgcaccac ggtctcgcgg agcgcgaggt gattctccgc
     4261 gatcacccgg atggggtagt cgcagggaaa ctcgattttc gggggttcag tcaacgccga
     4321 aaaggtcgcg aaagaggagc cgggtgccgt ccagcaggcg cgagaacatg tctgcttcct
     4381 tgacttcctg caacgcgacc aggggcgcgc tcaacatcag ctcgtcaccg aagtgcaaac
     4441 gcagttcccc cagctcctga ccggcagcta cgggagcctc gatggtcttc accacgctga
     4501 gctcggcctt gaggtcttcg tagtggcccc gggggatggt tacgaaaacc tcctctgcaa
     4561 ggcccagctg gatgtcgtcc gcctcgccgt accagatctt cgaggtcttc aaagggacac
     4621 cggcatcgta gagtttctgc gtctcgaaat agcgaaatcc gtaggaaagc agcttctggc
     4681 tctcgcgcat cctggcctct tcgctttccg tgcccagcac gacggaaatc aatcgcatgt
     4741 tgccgcgctt ggccgaggcg accaggcagt agccggcggc tgaggtgtgc ccggtcttga
     4801 ccccgtccac ggtgcggtcc cgccagagca gccggttgcg attgggctgc tcgatgtcgt
     4861 tgaaggcgaa agatcgctcg gagtagatct tgtagctgtc gggaaaccgc ttgatgagcg
     4921 actgggtgag caccgccaga tcccaggcgg aggtgtaatg atcgtcgctg ggaaggcccg
     4981 tggcgttctg atactgggtc gtggtcatgc cgagctggat cgcctgctga tccatcatgt
     5041 cagcgaaagc atcttcactc ccggcaatgt gctcggccat ggcgatgctg gcatcgttgc
     5101 ccgactgtac gatgatgcca cgaagcaggt cttccaaccc gacccgggtg ccctcccgca
     5161 cgaacatccg cgacccgggc gcacgccacg ctttgacgct gatgggcacc tgatcttcca
     5221 ggctgatccg gcctgcctcc agctctacgg cggcgatgta gccggtcatg atcttggtca
     5281 ggctggccgg tggcacgggc tcatgtattt cgttttccac cagcaccttc atggtgtccg
     5341 catctaccag gaggtaagag gtgccggcga tcttcggcgg cggcggaatg atgggcccct
     5401 cccccgcgtg ggcgggaccg gcaacagccg cgaggcccag acagaaggct gcgagatgat
     5461 tggcccagaa cgacatgaga agacctctca gcatgctcgt acgcttccgg tggattctag
     5521 ctcattgctc gagtatgagc gggcgatcga agtcagaaga cgcgatcagc gcctgcaaac
     5581 gaagcgcttc cggcctggac ttgaccggcc cgatacggac ccggaacaga gcatcgctgg
     5641 aaaccctcac cacataggcc cgcgctccgg tcagcgcgcc gagggcattc ttgagcgcat
     5701 cagccgagct catgtcgctg aaagcaccag cctgcagaaa gtagtcgggg ctatccacca
     5761 aagcctccac gcgcacccgc gccgtgcccc gggtcgcgaa gcccagcttc acggcggccg
     5821 cgtaggaaag gtcgatgagc cgatcggcgt gaaacggacc gcgatcattg atccgaacga
     5881 tagtggaacg cccgttgtgc agattggtca cccgggcgta ggtgggaatc ggcagcgaag
     5941 tgtgagcggc cgtcagctcg aacatgtcga acgtctcgcc gctcgacgtc gctcgtccat
     6001 ggaacttgcg gccgtaccag ctggcgattc ccgtggactc atagcccgcc gcgctgtcca
     6061 tcacccggta ggtgcgcccc atcaccgtgt agctcggcgg gttgccgcgg gcgcttttgg
     6121 gctcctcgcg gggtatcggg tcaggcagct tggcgaggtc aaccggcgtg tgcgcgggcg
     6181 gaccatcgct aagttctggc gggcgcacgc cagggccgct gcaggcaacc agggtgagca
     6241 ccagcagcca gcccgccgcg cgcaagcgga ctaccaagcc tgggtcaccg cgctggtgct
     6301 gcgctgccgc tccaagagct cctgactgag ctggtgaacg gccagcgcgt acagcc
//
LOCUS       DBNHKV010000234         4256 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1734842, whole genome shotgun sequence.
ACCESSION   DBNHKV010000234 DBNHKV010000000
VERSION     DBNHKV010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1734842"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1080
                     /locus_tag="AC16PP_07515"
                     /pseudo
     CDS             52..1080
                     /locus_tag="AC16PP_07515"
                     /inference="COORDINATES: protein motif:HMM:NF021535.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(1102..2412)
                     /locus_tag="AC16PP_07520"
     CDS             complement(1102..2412)
                     /locus_tag="AC16PP_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR6481511.1"
                     /translation="MYSTGTTPAAAITLGITLICVVWWVFEPIPIPATSVLPLAMLPL
                     LGVLTPQQVAQAYGHPLILLMLGGFILSTALARNGAHRRIALTMVRAFGGSSSRRLVF
                     GFMAASAVLSMWISNTATTLMLLPVALAVLEKAEDPKLATPLLLGIAYAASIGGIGTP
                     IGTPPNVVFMGVYNDLGVGQIGFADWMSFAMPVVLVLLPIAAVWLTRRLGHQGTVDVP
                     EVGAWQAGEVRVLMVFALTAVAWITRSEPFGGWTALLGLAQTNDAMVALLAVVIMFVV
                     PDGKGERLLDWETATQIPWGMLILFGAGLTIAAAFTASGLSQTIGALLSGLAALHVLL
                     IMLTICLTVTFLTETTSNTATTTLLMPILAAGAVGAGISPELFMIPAAMSASCAFMLP
                     VATAPNVIMFSTGRVAIETMVKEGFTLNLIGAIVIATLCYVLLT"
     gene            2509..3930
                     /gene="sbcB"
                     /locus_tag="AC16PP_07525"
     CDS             2509..3930
                     /gene="sbcB"
                     /locus_tag="AC16PP_07525"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HIR6481512.1"
                     /translation="MAASYYWYDLETSGIEPRWDRVIQFAGLRTDLSLNPVGDEYCTY
                     VELPDDVLPSPEATLVTGITPDVTRREGVSEWQAMTRINELFSAPQTCVAGYNSLRFD
                     DEFIRFGFYRMLIDPYAREWQNGNSRWDIIDLVRATGALRRDGIRWPTDDEGLPVYRL
                     ELLTKANDLEHGQAHDAMSDVRATVALARLIRDCQPKLFDYYFNGRSKKQVKQLLEPF
                     GARVCLHVSGMYPRSRYCAAPVVSLCRHPKNSNSIIVADLSEDVEPLIGWTDEQIRAE
                     LFKEGSTSRPPLKEIRINRCPFVAGIEVMTGENWERIGFDRREIKERQRRLARPGLAQ
                     KIMRVYGDRPPPAAPDVEAALYDAFLQDADRGRCASLQEALGQGKWLDLDYSDRRLVS
                     LSTRLKSRSFAESLSEEERQDWHEFVRDKLNAEDAPWLTLKEFDARLDRIRQDSAGED
                     ARRERLLGKLEAYAGGLKARYGG"
     gene            complement(4010..>4256)
                     /locus_tag="AC16PP_07530"
     CDS             complement(4010..>4256)
                     /locus_tag="AC16PP_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481513.1"
                     /translation="GPPDHIVKGIAERRLAVLLAGDSPADYYGAESFPWEENVCAVSP
                     VGDDGKWFVGVVRDAPVDIDFDPRLSSYDAYLATLER"
BASE COUNT          756 a         1339 c         1460 g          701 t
ORIGIN      
        1 ggggatcccg gcttgagaat gcagacgacc acagcaggcg tgcggccgca tatgacgatc
       61 gggcgcctgc tggcggcggc cgcggccgcc tcgtttctct ccggctgcgc gagcctcggc
      121 tactacaccc aggccatgag cggtcagctg cagatctcgt cttccgggca gcccgtgggc
      181 gaattgcttg cccgtctcga agccgttgag gaccccgatg cgcttcaggc cggtctcctc
      241 tcgggcctgc gctccagcca gtcggtgctc gctttcgcag aagccgaact cgggctcgaa
      301 gcaggcggta gctactcctc ctacgtcgag ctggatcggc cttacgtggt ctggaacgtt
      361 ttcgcggctc cggagctcag cctcgaggcg cggtcctggt gttatctcgt cgtcggctgc
      421 gctccgtacc gtggctactt cgagcagggc cgtgccgagc ggtttgcggc aggtctcaag
      481 gacaggggcc tggacgtcta cgtggggggc gtgcccgcgt actccacgct cggctggttc
      541 gaagacccgc tgctgagcac gttcattcgc tggccccaag gaaggctggc cgagttgctg
      601 attcacgagc tggctcactc cagggtctgg gtggcgggtg acgtggggtt caacgaagcg
      661 tttgcgagct tcgtgggccg agagggcgcc cggagctgga tcgagcagcg ccatggggcc
      721 caagcggccg atgagtttct cgagcgcggc aggagctggc cggcactgat gcggcttctg
      781 ctcgagatcc ggcgggctct ggaggagctt tacatcagcg gtctgagcga aggggaaaaa
      841 cgggcgggca agtcgaaggt gatcgatcgt gggaaagcct gttaccagag gaacatttcc
      901 cgatacggat ccggaaggta cgacgaggtt ctggccgggc tgaacaacgc gtttctcgtt
      961 tccctgtcga cttacgacga cgacgttccg gccttcgccg ccgtcttcgc ccaggtgtcc
     1021 ggagattggc gacgcttctt cgacgaggtc gagacgatgg cccgcatgac ctccgagcgc
     1081 agggccgatg cgatggaagc cttacgtgag cagcacatag cagagcgtgg cgatgacgat
     1141 ggcgccgatg aggttgagtg tgaagccctc tttcaccatg gtctcgatgg cgacacggcc
     1201 ggtgctgaac atgatcacgt tgggcgcggt ggccacgggt agcatgaagg cgcaggatgc
     1261 gctcatcgcg gcaggaatca tgaagagctc gggcgatatg cccgcgccca cggcgcctgc
     1321 ggcgagaatc ggcatcagca gggtcgtcgt cgccgtgttg ctcgtggttt cggtgaggaa
     1381 ggtaacggtg aggcagatgg tgagcatgat cagcagcaca tgcagcgctg ccaggccgga
     1441 cagcagcgcg ccgatcgtct ggctgagacc ggaggcggtg aacgccgcgg cgattgtcag
     1501 ccccgcgccg aacagaatca gcatgcccca ggggatctga gtagcggtct cccagtcgag
     1561 caaccgctcg cccttcccgt caggcacgac gaacatgatc accacggcga gcagggcgac
     1621 catggcgtcg ttggtctggg ccagccccag cagtgccgtc cagcctccga acggctcgct
     1681 gcgggttatc caggcgacgg cagtcagggc gaagaccatc agcacccgaa cctcaccggc
     1741 ctgccacgcg cccacctcgg ggacgtccac ggtgccctgg tgtcccagtc gacgggtcag
     1801 ccacacggcg gcgatgggca ggagcaccag caccacgggc atcgcaaacg acatccagtc
     1861 cgcaaacccg atctgaccca cgccgagatc gttgtacacg cccatgaaca cgacgttggg
     1921 cggggttccg atgggcgtgc cgatgccgcc gatgctggcg gcgtaagcga tgcccagcag
     1981 gagcggcgtg gcaagcttcg gatcctcggc cttttccagc accgcgagcg ccacgggcag
     2041 aagcatcagc gtggtcgcgg tgttggagat ccacatgctc agcaccgcgc tggcggccat
     2101 gaacccgaaa accagtcgcc ggctgctgct gccgccaaaa gcgcggacca tggtcagcgc
     2161 gatgcgcctg tgggccccgt tgcgggccag ggctgtggag aggatgaacc cgccgagcat
     2221 gagcaggatg agcggatgac cgtaagcctg ggcgacctgc tgcggcgtca gcacccccag
     2281 caggggcagc atggccaggg gaagcaccga cgttgccggg atggggatag gctcgaacac
     2341 ccaccacacc acgcagatca acgttatgcc caaggttatg gccgccgccg gggtcgtccc
     2401 ggtagagtac atgaggtatc cgacgaacaa tgcgagaatg gggccagcgg ggagcgcgat
     2461 gcgctttgcg ttggaagccg acatgggaag gcaaggttat aggaagcgat ggcagccagc
     2521 tactattggt acgaccttga aacctcgggt atagagccgc gctgggatcg cgtgatccag
     2581 ttcgcagggc tgcgcaccga tctgtcgctc aacccggtcg gggacgagta ctgcacctac
     2641 gtggagctcc ccgacgacgt gctgccgagt cccgaggcga cgctggtgac gggcattacc
     2701 cctgatgtca cccgccggga aggcgtcagc gagtggcagg cgatgacgcg catcaacgag
     2761 cttttctcgg cgccccagac ctgcgtggcc ggctacaaca gccttcgctt cgatgacgag
     2821 ttcattcgct tcggcttcta ccggatgctc atcgacccgt acgcccggga gtggcagaac
     2881 ggcaactcgc gctgggacat catcgatctc gtccgggcga cgggcgcgct gcggcgcgac
     2941 gggatccgct ggcccaccga cgacgaaggt cttcccgtct accggctcga gctgctcacc
     3001 aaggccaatg acctcgagca cggccaggcc cacgatgcca tgtcggacgt gcgcgctacg
     3061 gttgcgcttg cgcggttgat tcgcgactgc cagccgaagc tgttcgacta ctatttcaac
     3121 ggccggtcga agaagcaggt caagcagctg ttggaaccgt tcggagcgcg cgtatgcctg
     3181 catgtctcgg gcatgtatcc gcgcagccgc tactgcgccg cgccggtggt gtcgctgtgc
     3241 cgccacccca agaacagcaa ctccatcatc gtcgccgacc tcagtgaaga cgtggagccg
     3301 ctgatcggct ggaccgacga gcagatacgt gcggagctgt tcaaggaggg ttccaccagt
     3361 cgccccccgc tgaaggagat ccgcatcaat cgctgcccgt tcgtggcggg catcgaggtc
     3421 atgaccggcg agaactggga gcgcatcggg ttcgaccgtc gagagatcaa ggaacgccag
     3481 cggcgtctcg cccggcccgg actggctcag aagatcatgc gcgtatatgg cgatcgcccc
     3541 ccaccggcag cacccgatgt ggaagcggcg ctttacgacg cctttctcca ggatgcggat
     3601 cgcggtcgct gcgccagcct tcaggaggcg cttggccagg ggaagtggtt ggacctcgac
     3661 tattcggacc gccggctggt ttcgctctcg acccggctga agtcccgcag ctttgcggag
     3721 tcgctctccg aggaggagcg gcaggactgg cacgagttcg tgcgcgacaa gctcaatgct
     3781 gaggatgcgc cgtggctcac gctgaaggag ttcgacgcgc ggctggatcg gatccggcag
     3841 gactcggccg gagaggacgc ccgacgcgag aggctgctcg ggaagctcga agcctacgcc
     3901 ggggggctga aagcacgcta cggcggctga cacggtccaa ggccgtcccg gcggcccgga
     3961 ccgaggtgcc agccggtcgg gtagcgccgg ccccagtcgc gatgccgctt cagcgctcca
     4021 gggttgccag gtaggcgtcg taggaggaaa gcctcgggtc gaaatcgatg tctaccggcg
     4081 cgtctcgcac cacgccgaca aaccacttcc cgtcatcgcc cacggggctg accgcgcaga
     4141 cgttctcttc ccacggaaag ctctcggcgc cgtaatagtc ggccggggag tcgccggcga
     4201 gcagcacggc cagccggcgc tccgcgatgc ccttcacgat gtggtcgggg gggccc
//
LOCUS       DBNHKV010000235        15493 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1237410, whole genome shotgun sequence.
ACCESSION   DBNHKV010000235 DBNHKV010000000
VERSION     DBNHKV010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 15493)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15493)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15493
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1237410"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="AC16PP_07535"
     CDS             complement(<1..774)
                     /locus_tag="AC16PP_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018404836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR6481514.1"
                     /translation="MKRANAFYAQSGGVTAVINASACGVIQTARKHRDRIGKVLAGRN
                     GIIGALAEELIDTGRESAKDIAGLRTTPSGAFGSCRYKLKSLEENEREYRRLIDVFRA
                     HDIGYFFYNGGGDSQDTAHKVSQLGGRLGYPITCIGIPKTVDNDLPFTDCCPGFGSVA
                     KYVAVSTREAAMDVASMCETSTKVFILEVMGRHAGWIAAAGALAQEGPRDAPHIILLP
                     EVPFDRKRFLAKVRETVDGCGYCVIVVSEGAQYPDGTFLA"
     gene            847..1347
                     /locus_tag="AC16PP_07540"
     CDS             847..1347
                     /locus_tag="AC16PP_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481515.1"
                     /translation="MKAVLQLFWQICRLKQSPEYVPTQTWFVLLVIAANLICSAAVSL
                     SAEGAPGLLTTINSIVVGQTTTAALVWVALNLRDMGGRFLATITALFGCDLIITAVFA
                     LILPLTLLAPTMAPVLFLLFVAWSVSVAGFILHRALQVPLALGIVVALGMSVLSLALS
                     QLAIGA"
     gene            1344..2747
                     /gene="mpl"
                     /locus_tag="AC16PP_07545"
     CDS             1344..2747
                     /gene="mpl"
                     /locus_tag="AC16PP_07545"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HIR6481516.1"
                     /translation="MNAERRIYFLGIGGTLMGSLAQLAREMGYRVSGSDVAIYPPMSD
                     QLAAADIEVFEGFDPAHLDPEPELVVIGNAGLPRGHPGVEHIIENGLRYVSGAEWLGS
                     QVMPGRWVLAVTGTHGKTTTASMLAWILDQAGLKPGYLIGGVPKNFPRSSRLGDDPFF
                     VVEADEYDCSYFDRRSKFVHYHPRTLIINNLEYDHADIFPNLAAIQTQFNHLLRTVPG
                     HGLVIAPSNDDNVNETLQQGCWTPIARVGAPGVKQPLERDNGERWSASRGDGTGAEFD
                     VCLGEENLGTVRWPIFGDFNVRNALAAIAAARHAGVPPEVAIDALGSFEGVKRRLELI
                     AEIGATLIYDDFAHHPTAIRATLQGLRNKVGSEEIIAVIEPRSHTMSLGTLRNELATC
                     CSPADEVYWFRGENIQWDLSELLKSCVIPAHINFELDKLIEALAQLPQKRRHIVIMSN
                     GSFGGIYQKLPTRLRSG"
     gene            2792..3727
                     /locus_tag="AC16PP_07550"
     CDS             2792..3727
                     /locus_tag="AC16PP_07550"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR6481517.1"
                     /translation="MQSRPACFVAALLLAFVPAANAADWYPSRYGADDTLGAINNLSP
                     EKVVQAARLVTEGKTYPLGVETGPDSPAYPPRSYSLTVLQPGDGTGSLMGTNRASGND
                     DLLSFWMGIGSQIDGLGHMGIEHVYYNGNRAQDFVSATGLSRLSIDRLPPIVGRGVLI
                     DMAAHMGKAILPAGTAFNSREIKAAAKAQGVTIGTGDVVLFHTGWLNVADDKARFMAG
                     EPGLGVDGARYLAGLGVVAVGSDTWGLEVLPSEVEGEAFPVHPELLAKNGVYILENMD
                     TRALAADGVHEFLFVLGQPRFVGAVQAVINPVAIK"
     gene            3727..4410
                     /locus_tag="AC16PP_07555"
     CDS             3727..4410
                     /locus_tag="AC16PP_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481518.1"
                     /translation="MKAPRRPTAQAAIRAAAVAGLLLAGCDTGSGAEDTAQAQARTGA
                     EPASPELLMARPPEGWKVWSATQDPGLRVAQYVPADTDADNWEQMLRLESMAGDPLPD
                     PIEFLTALGRDQAAGCGQSSDLNIHSGQENNYATSVRLFSCRNDAAPDLDQVTLIKAI
                     RGNENFYVIALTRRTTSGESTRPPVSDAEMAVWAAYMRTVTVCDPTRPEHPCPTSGEA
                     PEQPAATAG"
     gene            complement(4407..6143)
                     /locus_tag="AC16PP_07560"
     CDS             complement(4407..6143)
                     /locus_tag="AC16PP_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015502313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6481519.1"
                     /translation="MLEYQAPLRDFDFLLFDVFRAGETWGRIPAFAELSEELVRAVLA
                     EGGRVAREVMAPLNQTGDEQGCRWDDGVVTAPAGFKAAYDDMAGGGWLGISGNPEFEG
                     QGMPKMLGCLVEEMFWSANTSLFLYGTLSVGASICIDTHGSAAQKSLYLPKLYSGRWS
                     GAMDLTEPHAGSDLGMMRTRAESAGDGTYRITGTKIFITSGEHDLSENIVHLVLAKVP
                     GAPAGSRGISLFIVPKFLPDADGEPGERNALASGSIEHKMGIRGSATSVMNFDGAVGF
                     LVGEENQGLAAMFTMMNYERLSVGLQGLGAGELAYQQAARYATERIQGRSPEGPQKPE
                     AAADPLIVHPDVRRMLLTIRCLTEAGRAFGLFAGMQLDLGKHLGDPDALALGDLLTPI
                     AKAFLSDRGLDCAVLGQQVFGGHGYVREWGMEQIVRDARIAQIYEGTNGIQAQDLIGR
                     KILRDGGAVLRRLATIVEADARDSAYRADLLAAVARLVKVTDSLVQRSRADAALPGAV
                     AVDYLDLAGYTLYAWLWARMADAAPGDDFGRSKRACAEFYFKRLLPRTLGLAAAIEAE
                     SEAVMALAADAF"
     gene            complement(6192..7958)
                     /locus_tag="AC16PP_07565"
     CDS             complement(6192..7958)
                     /locus_tag="AC16PP_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014590723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6481520.1"
                     /translation="MPASLKDVRFLIHDVFDFAGHYARLELDEPPGRDLIDAIVEEAA
                     RFADGELAPLNRVGDESGCRLEDGSVSTPAGFKEAYAKYVEGGWAGLSGSAAYGGQGL
                     PDSMSFFLEDLMCCANMAWTMYPGLSRGVVGALEEHGSAELKDAFLANLLSGEWTGTM
                     CLTEPHAGSDVGLARTRAEASADGTYRISGTKIFISAGEHDMAENIVHLVLARIPDAP
                     PGTKGISMFVVPKFDLDGTRNGVSCGSIEEKMGIHGNATCVLNFDGARGYLVGEPNHG
                     MRYMFTMMNGARLVVGLQGASQAHAACLLSLEYAQDRPQMRSLTGPKAPERPADPIIV
                     HPDVRRMLLFQRAIAEGGRALVYYAAQIIDRFRDGSPDERASAGELLDFLTPVVKGIL
                     TELGFESVNQAVQIYGGHGFIRETGVEQYVRDTRITTIYEGTTQIQALDLVGRKILQN
                     QGKGLVSFIGEMQAVAASLEDPLPRLAAPLKALADEWGELTLQLSATAMQDLDEVGAA
                     AVDYLYYSGYAALAFCWALMARSADEAIRAGSTDPFHAGKIAVADFFFSRVLPRAKAH
                     QAAIEAGKGTLMDVDEGALVAL"
     gene            8165..8860
                     /locus_tag="AC16PP_07570"
     CDS             8165..8860
                     /locus_tag="AC16PP_07570"
                     /inference="COORDINATES: protein motif:HMM:NF039185.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR6481521.1"
                     /translation="MIGRALRALFPPICLLCDQSCAAEADVDLCSRCLPVLPWNPQPC
                     PRCALPRENGTGRCQPCEDDPLPVARTVAPLLHAGYPRHWVHRLKFHEGMVEGRLLAH
                     LLAGAVRESYGRTDLPDMLVPVPTAPGRLAWRGHNQAATIAAFLSRRLSLPLARRGAR
                     RARSSRPQRLLSRAARLKATAGIFESRQWRGETIAVVDDVMTTGATVAELARTLTAAG
                     AGEVHVWSATRAV"
     gene            8883..9869
                     /locus_tag="AC16PP_07575"
     CDS             8883..9869
                     /locus_tag="AC16PP_07575"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR6481522.1"
                     /translation="MSESSTSTPYAGLTPDLILDALEATGFAPTGSLLELNSYENRVY
                     QLEVEDGTFVVTKFYRPERWSDAQILEEHAFTAELIEHELPAVGPLRRDGTTLFEHSG
                     FRFAVFPRQGGHPPNIEVEEDLKVLSRTLARLHAIGRIRPFETRPTLSCERMGRQSRE
                     FLLETNLVPLELQPAYASTTEHLLERLEPLFDTEHQWRRIHGDCHLGNLLWRGEVPHF
                     VDFDDCVNGPPIQDLWMLLSGEHHEREAQLSMILEAYDQFASFDLSSLRLIEPLRTLR
                     IMHHAAWIGRRWSDPAFPRAFPWFNTEKYWSEHILSLREQMAALDEPTLTVS"
     gene            complement(9850..10986)
                     /locus_tag="AC16PP_07580"
     CDS             complement(9850..10986)
                     /locus_tag="AC16PP_07580"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR6481523.1"
                     /translation="MLDFEAIRPYEDPEVAGVVARLVEDAELDRAVAVYLMPGLARVW
                     PRLASVLVRAYLRRKTKGLVTVNDVQLFLGKYMARLIEETVVELTVDGLDELSKGKPY
                     LFMSNHRDIVMDSGILNYVIHRAGHQTSRIAVGDNLFARPFAADLMRLNKSFVVERKV
                     SGTRAVYAALSRTSHYIRQSLEEGESVWIAQREGRSKDGFDRTDPALLKMLGLAYRKE
                     AGSFGDLVTRIRIVPVAVSYELDPCDLRKAHELYLTEREGSYIKPPDEDFQSIVEGMM
                     GFKGRVHLHFGSPVTGYFETAEALALEIDRAIVAGLKIFPTQAQAAARLGQASCHVQL
                     AELPAVTAAFEQRLAGCPDAEKPYLLAAYANVLRNRRELSLPSA"
     gene            11085..12050
                     /locus_tag="AC16PP_07585"
     CDS             11085..12050
                     /locus_tag="AC16PP_07585"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6481524.1"
                     /translation="MGLGIRPFDKGLLDLGNGAYAWLQPDGSWGWSNAGLVVDGDASL
                     LVDTLFDKHLTREMLSAMRDAAPKATRHFDTLVNTHSNGDHCNGNELVTGAEIVASKA
                     CAEELAAENPRMMQTMMERAPEMGEVGEFFVHCFGAFDFTNINHTLPTRTFEGELGLT
                     VGDKAVLLKQVGPAHTRGDVLAYVPDEKLIYTGDILFIEGHPILWVGPVGNWIEACDY
                     MLGLDLENVVPGHGPVTDKRGVMAVRDYLIYIRDEARKRFDAGMPPDEAAVDISLTDY
                     DSWGDAERIVVNVAVLYKEFAADPEPGDIAELFALMAKVHKARRR"
     gene            12047..12538
                     /locus_tag="AC16PP_07590"
     CDS             12047..12538
                     /locus_tag="AC16PP_07590"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6481525.1"
                     /translation="MTDLRRAGEDDLPALVRIYNHYVAHSHCTFDIETYSVDERRGWW
                     RRFDAARYQCWIAEHAGRVIGYACSQPLKAKGGYETSVEVSVYVDAAHTGSGTGRSLY
                     QQLLAALAPEDVHRAYALVALPNDPSIALHQTMGFREVAHLGEVGRKFGRYWDVVWLE
                     RAL"
     gene            complement(12535..13287)
                     /locus_tag="AC16PP_07595"
     CDS             complement(12535..13287)
                     /locus_tag="AC16PP_07595"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR6481526.1"
                     /translation="MDPWTLGIFLGGALFGSYVQSVTGFAMAMILVAVASAAGNSQVS
                     VVAAVVSLLALVNVALALRGHLERVEARLFVWLAVGQLPAIWAGIYLLEVLEGQAVRA
                     LEVVLGVFIVLGSLSMMLRPQPRPSTSPPWACVAAGVTGGLAGGLFAASGPIMGWFNY
                     RQPLPVAAIRATLLCGFAMTTSVRTVLVGVNGGLTGEVWLLTGLALPVVVLGTWAGRT
                     FEPPVSELAMKRLAFAVLLVMGIWILLRAALG"
     gene            13369..14145
                     /locus_tag="AC16PP_07600"
     CDS             13369..14145
                     /locus_tag="AC16PP_07600"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6481527.1"
                     /translation="MDLGLTGKTALVSGSYRGTGLVIARHLAAEGVRVFVHGLEPGQA
                     ERAVEEIGSGDPVTGDITCDAGAREVAEALGDSRVDILVNNYGTADRGTWQDSDTDDW
                     LDAYQKNVLSAQRLIRAYFAPMRERGWGRIINLGTVGSTRPNSRMPHYYASKGALATM
                     TISLAKEASGTGVRVNLVSPGLILTPEVEAAYLERGRAKGWGETWEEVEPNVAADIPI
                     GRIVRREEVADLVTFLASPLADAIHGQNIRVDGGALDVLS"
     gene            14142..14528
                     /locus_tag="AC16PP_07605"
     CDS             14142..14528
                     /locus_tag="AC16PP_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481528.1"
                     /translation="MTGPGLTRPGIRLLGLAALGLCVLAYDAGSYTLLHRLVLPIAMG
                     VAAGLMVQSAAAVLLAGTLLAGVHARPADPDWVIGIAYPALAALCGVGLTWIVVLRFR
                     RRIKATREARWRGRRERSAAPEGSES"
     gene            14530..15381
                     /locus_tag="AC16PP_07610"
     CDS             14530..15381
                     /locus_tag="AC16PP_07610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6481529.1"
                     /translation="MGTRCFLVSGTTSGIGLATARALAATGGTVVTANRDPDRGESTA
                     ALLRDATSNPNVINTVCNLSSLASVRSCAARIREQFPRIDVLINNAGVWTAKPQLSED
                     GFELSFATNHLGPFLLTHMLLEPLRAAGGARIVNVASVQHRAADLDLDHLTPKTPYRG
                     LRAYSRSKLANVMTTLAFARRFDAAEISANCLHPGVVASNIVPENSPLLRRAARVVSG
                     LMRSPARGAATSIYLATSPEVTGVTGKYFDPDQRIIQPSAEARDEAAQDLLWERSLEL
                     TGLAEAG"
BASE COUNT         2742 a         5309 c         4941 g         2501 t
ORIGIN      
        1 ggccaggaag gtgccgtcgg ggtactgagc tccttcggag acgacgatta cgcagtaacc
       61 gcacccgtcc acggtctccc gaaccttggc gagaaaacgt ttccgatcga agggcacctc
      121 cggcagcagg atgatgtgag gcgcatcgcg cggcccttcc tgggccaggg ctcccgccgc
      181 cgcgatccaa cccgcgtgac gccccatgac ctccagaatg aagaccttgg tggaggtctc
      241 gcacatcgac gcgacgtcca tggccgcttc gcgggtggat accgcgacgt acttggcaac
      301 ggatccgaac ccgggacagc agtccgtgaa cggaagatcg ttgtcgaccg tcttgggaat
      361 cccgatgcag gtgatcggat atccgagccg cccgcccagc tgggacacct tgtgggcagt
      421 gtcctgggaa tccccgccgc cgttgtagaa aaagtaacca atgtcgtggg ctcgaaagac
      481 gtcgatcagg cgccggtact cgcgctcgtt ctcctctaag gacttcaact tgtaccggca
      541 ggatccgaaa gccccgctcg gcgtcgtgcg cagcccggca atgtccttgg cgctctcccg
      601 gcccgtgtcg atcagctcct cggcgagcgc gccgatgatc ccgttgcgtc cggcgaggac
      661 cttaccgatg cgatcgcgat gcttgcgcgc ggtctggatg accccgcatg cggatgcgtt
      721 gatgacggcc gtgaccccgc ccgactgagc gtagaacgca ttggctctct tcatccctcg
      781 acgtctcgtc cggaaaacga gcggtcattg tccgtgaaga gaaagttttg tgcgacaatt
      841 tcgccgatga aagccgtctt gcagcttttc tggcagatat gccggctgaa gcagagtccc
      901 gagtacgtac ccacccagac gtggttcgtg ctgctggtca tcgccgcaaa cctgatctgc
      961 agcgctgccg tttctctgtc cgccgaaggc gcgcccggcc tgctgaccac catcaacagc
     1021 atcgtcgtgg gccagacgac gacggcggcc ctggtgtggg tggcgttgaa cctgcgcgac
     1081 atgggtgggc ggttccttgc aaccatcacg gcgctgttcg gctgcgacct catcatcacg
     1141 gcggtcttcg ccctcatcct gccgctcacc ctgctcgcac cgaccatggc gcctgtgctg
     1201 ttcctgctct tcgtcgcctg gagcgtgagc gttgccggct tcattctgca tcgcgcgttg
     1261 caggtcccgc ttgcgctcgg gatcgtcgtg gccctgggca tgtcggttct cagcctggca
     1321 ttgagccagc tggccatcgg cgcatgaacg cagaacgccg aatctatttt ctgggcatcg
     1381 gcggcaccct gatggggagc ctggcccagc tggccaggga gatgggatac cgggtctccg
     1441 ggtcggacgt ggccatctac ccgcccatga gcgatcagct ggcggccgcc gacatcgagg
     1501 tgttcgaagg cttcgatccc gcccacctgg atcccgagcc ggagctggtg gtgatcggca
     1561 acgcgggcct gcccaggggt catccgggcg tcgagcacat catcgagaac gggctgcgct
     1621 acgtgtccgg tgccgaatgg ctcggcagcc aagtgatgcc cggccgctgg gtgctggccg
     1681 tcacgggcac ccacggcaag accacgacgg ccagcatgct ggcctggatt ctcgaccagg
     1741 cggggctgaa gcccggctac ctgatcggcg gcgtgcccaa gaactttccg cgctcgtccc
     1801 ggctgggcga cgaccccttt ttcgtggtgg aggccgacga gtacgactgc tcgtacttcg
     1861 accgcagatc caagttcgtt cactaccatc cgcgcaccct gatcatcaac aatctggagt
     1921 acgaccacgc ggacatcttc cccaacctcg ccgccatcca gacccagttc aatcacctgc
     1981 tgcgcaccgt gcccggccac gggctcgtca tcgcgccgtc caacgacgac aacgtcaacg
     2041 agaccctcca gcagggttgc tggacgccca tcgcccgcgt gggcgcaccc ggggtgaagc
     2101 aaccgctgga acgcgacaac ggtgagcggt ggagcgccag caggggagac ggcacaggtg
     2161 ctgagttcga cgtctgcctg ggcgaggaaa atctgggcac ggtgcgctgg cccatcttcg
     2221 gcgacttcaa cgtccgcaac gccctggccg ccatagctgc cgcccggcac gccggcgtgc
     2281 cgccggaggt cgccatagac gccctgggca gcttcgaagg cgtcaagagg cgcctggagc
     2341 tcattgccga gatcggcgca acgttgatct acgacgactt tgctcaccac cccaccgcca
     2401 tccgcgcaac gctgcagggc ttgcgcaaca aggtaggcag cgaggagatc atcgccgtca
     2461 tcgagccccg ctcccacacc atgtcactgg ggaccctgcg taacgagctc gccacgtgct
     2521 gctcgccggc ggacgaggtc tactggtttc gcggggagaa cattcagtgg gacctctcgg
     2581 agctgctgaa gagctgcgtg atccctgcgc acatcaattt cgagctcgac aagctcatcg
     2641 aggccctggc ccagctgccc cagaagcgcc gccacatcgt gatcatgagc aacggcagct
     2701 tcggcgggat ctaccaaaag ctcccaaccc gtctgcgcag cggctaaact cgggcaccac
     2761 ttccagccca ctttcgccag gaggccccgc catgcaatcc agaccggcat gcttcgtcgc
     2821 agcgctgctg ctcgcattcg tccctgctgc caacgcagcg gattggtacc cgtcgcgcta
     2881 cggcgccgac gacaccctgg gggccatcaa caacctgtct cctgagaagg tcgttcaggc
     2941 ggcccgtctg gtcacggaag gcaagaccta cccgctgggg gtggagaccg gccccgactc
     3001 gcccgcctac ccgccgcgaa gctattcgct caccgtgctg cagcccggcg acggcaccgg
     3061 atccctgatg ggtaccaaca gggcgtccgg caacgacgat ctgctgagtt tctggatggg
     3121 catcggcagc cagatcgacg ggctcggcca catgggcatc gagcacgtgt actacaacgg
     3181 caaccgggcc caggatttcg tgagcgcaac cggcctgtcg aggctgagca tcgaccgcct
     3241 gccgcccatc gtcggccgcg gcgtgctgat cgacatggcc gcccacatgg gcaaggccat
     3301 tctccccgcc ggcaccgcct tcaacagccg agagatcaag gccgcagcca aggcccaggg
     3361 cgtaaccatc ggcacgggag acgtggtgct tttccacacc ggctggctga acgtcgccga
     3421 tgacaaagcg cgcttcatgg ccggcgagcc cggtctgggc gtagacgggg cacgctatct
     3481 ggccggtctc ggcgtcgtgg cggtggggtc ggacacctgg gggctcgagg tgctgccttc
     3541 cgaggtcgaa ggcgaagcct ttccggttca tcccgagctg ctcgccaaga acggcgtcta
     3601 catcctggag aacatggaca cccgggccct ggccgcggac ggcgtgcacg agttcctctt
     3661 cgtgctcgga cagcctcgct tcgtgggcgc cgtccaggcg gtgatcaatc cggtggcgat
     3721 caagtgatga aggccccgag gcgcccgacc gcccaggccg cgatccgggc ggccgccgtg
     3781 gccggcctgc tgcttgccgg atgcgacacc ggctccggcg ccgaggacac cgcgcaggca
     3841 caggcccgaa ccggcgccga gccggcttcc ccggagctgc tcatggcccg accaccggag
     3901 ggctggaagg tatggtctgc cacccaggat ccggggctgc gagtggccca atacgtcccg
     3961 gccgacaccg acgccgacaa ctgggagcag atgcttcgcc tggaatccat ggcgggggat
     4021 ccgctgccgg atcccatcga atttctgacg gcgctgggca gggatcaggc ggccgggtgc
     4081 ggacagtcat cggatctgaa cattcattcc ggccaggaga acaactacgc cacgtccgtc
     4141 cggctcttct cctgtcgcaa cgacgcggct ccggacctgg accaggtgac cctgatcaaa
     4201 gccatccggg gaaacgagaa tttctacgtc atcgcgctga ccagacggac aacctccggg
     4261 gagagcacgc gaccgcccgt cagcgatgcg gagatggcgg tgtgggcggc ctacatgcgc
     4321 acggtgaccg tctgcgaccc cacccgtccc gagcacccct gcccgacgtc gggcgaggcg
     4381 cccgagcagc ccgccgccac ggccggctag aacgcgtcgg cggctagcgc catgaccgcc
     4441 tcggattccg cctcgatggc agcggcgagg cccagcgtcc gcggcaaaag ccgcttgaag
     4501 taaaactcgg cacaggcccg cttgctgcgg ccaaagtcgt caccgggggc cgcgtccgcc
     4561 attcgggccc acagccaggc gtacagggta taaccggcga gatcaaggta gtcgacggcc
     4621 accgctcccg gcagagcggc atcggcgcgc gaacgctgca ccagagagtc cgtgaccttc
     4681 accagccgcg cgacggcagc cagcagatcc gcgcggtacg ccgagtcgcg ggcatcagcc
     4741 tcgacgatgg tggcgagccg gcgcagcacc gcaccaccgt cgcgcagaat cttccgcccg
     4801 atcaggtcct gggcctgaat gccgttggtg ccctcgtaga tctgcgcgat gcgggcatcc
     4861 cggacgatct gctccattcc ccactcacga acgtatccgt gcccgccgaa gacctgttgt
     4921 cccaggacgg cgcaatccag gccgcgatcg ctcagaaacg ctttggcaat cggggtgagc
     4981 aggtcgccaa gggccagggc gtccggatca cccaggtgct tgcccagatc gagctgcatg
     5041 ccggcgaaga gcccgaacgc ccgacctgct tcagtgagac atcggatcgt gagcagcatg
     5101 cggcgcacgt ccggatgaac gatcagcggg tccgcggcag cctcgggttt ttgcggtccc
     5161 tccggggagc gaccctgaat tcgctcggtg gcataacgcg ccgcctgctg gtaggcgagc
     5221 tcccctgcgc ccaggccctg caggcccacc gagagccgct cgtaattcat catggtgaac
     5281 atcgcggcga ggccctggtt ctcctcgccg acgagaaagc cgaccgcgcc gtcgaagttc
     5341 atgaccgacg tggcgctgcc acgaatgccc atcttgtgct cgatggatcc gctggccagc
     5401 gcgttgcgct cgccgggctc gccatcggcg tcgggcagga acttcggcac gatgaacagc
     5461 gatatgcccc ggctgcccgc cggcgccccc ggaacctttg ccagcaccag atgaacgatg
     5521 ttctcggaca ggtcgtgctc gccgctggtg atgaagatct tcgtgcccgt gatccggtag
     5581 gtgccgtcgc ccgccgactc cgcccgcgtg cgcatcatgc cgagatccga gcctgcgtgg
     5641 ggctcggtga ggtccatggc gccgctccag cggccgctgt agagctttgg caggtagagc
     5701 gatttctgcg cagccgagcc atgggtgtcg atgcagatcg aggcccccac cgaaagcgtg
     5761 ccgtacagga acaggctcgt gtttgcggac cagaacatct cctcgaccag acatccgagc
     5821 atcttcggca tgccctgacc ctcgaactcc ggattgcccg agatccccag ccagccgccg
     5881 ccggccatgt cgtcgtatgc ggctttgaag cccgcgggcg cggtgacgac gccgtcgtcc
     5941 caacgacagc cttgctcgtc tccggtttgg ttgagcggtg ccattacttc tctggcgacc
     6001 cttcccccct cggccagcac ggcgcgaacg agttcttcgg atagctcagc aaatgcggga
     6061 atacggcccc aggtctcccc cgcccgaaac acatcgaaca gaagaaagtc gaagtctctc
     6121 agcggggcct gatactcgag catgggttcc ccctgccgtc caaggcaatc atgacttcaa
     6181 gcgcacggtg ttcagagcgc gaccagcgca ccttcgtcga cgtccatgag agtccctttg
     6241 cccgcttcga tggcagcctg gtgtgccttc gcgcgcggca gaacgcgact gaagaaaaag
     6301 tcggcgaccg caatcttgcc tgcatgaaag ggatccgtcg accccgctcg aatcgcctcg
     6361 tccgcgctgc gcgccatgag agcccagcag aaagcgagcg ccgcgtaccc gctgtagtag
     6421 aggtagtcca cggccgcagc acctacctca tccagatcct gcatggccgt cgcgctgagc
     6481 tgcagcgtga gctcgcccca ctcgtcggca agcgctttga gaggagcggc cagacggggc
     6541 agcggatcct ccagggaggc ggcaacggcc tgcatctcgc cgatgaagct gacgagcccc
     6601 ttgccctggt tctgcagaat tttccgaccc acgagatcga gggcctgtat ctgcgtggtg
     6661 ccctcgtaga tggtggtgat tctggtgtcg cgcacgtact gctcgacacc ggtctcccgg
     6721 atgaagccgt ggccaccgta aatctgcacg gcttgattga ccgactcgaa gccaagctcc
     6781 gtcaggatac ccttgaccac gggggtcaga aaatcgagca gctcgccggc cgaagcccgc
     6841 tcatccgggg acccgtcccg gaaacgatcg atgatctgtg cggcgtagta gacgagcgcc
     6901 cgtccccctt ccgcgatggc gcgctggaag agcagcattc gccgcacgtc cgggtgcacg
     6961 atgatgggat ccgccggccg ctccggcgcc ttgggccccg tgagggagcg catctgcggg
     7021 cgatcctggg cgtattccaa cgacagcagg caggccgcgt gggcctggct ggcgccctgc
     7081 agacccacca ccagccgcgc gccgttcatc atcgtgaaca tgtaacgcat gccgtggttc
     7141 ggctcgccga ccagatatcc cctggcgccg tcgaagttga gaacgcaggt ggcgttgccg
     7201 tggatgccca tcttctcctc gatggagcca cacgacacgc cattgcgcgt gccgtcgaga
     7261 tcgaacttgg gcacgacgaa catggatatg cccttggttc ccggaggcgc gtccggaatg
     7321 cgggcaagaa ccaggtggac gatgttctcc gccatgtcgt gctcgccggc agagatgaag
     7381 atcttggtcc ccgagatccg gtacgttccg tctgcagagg cctccgcccg ggttctggcc
     7441 aggcccacgt ccgacccggc gtggggctcg gtcagacaca tggtccccgt ccactcaccc
     7501 gagaggagat tggcgagaaa cgcgtccttg agctccgcgc tcccgtgctc ctctaaagcg
     7561 ccgacgaccc cccgcgaaag gcccgggtac atggtccagg ccatgttcgc gcagcacatc
     7621 aggtcctcca ggaaaaagct catgctgtcc ggcaggccct ggccaccgta ggcagcggaa
     7681 ccgctgagac cggcccagcc tccttcgacg tacttggcgt aggcctcctt gaacccggcc
     7741 ggcgtgctca ccgacccatc ttccagtcgg cagccggatt cgtccccaac gcggttgagc
     7801 ggcgccagct cgccatcggc aaaccgcgca gcttcctcga cgatggcgtc gatgagatcg
     7861 cggcccggtg gttcgtcgag ctcgagccgt gcgtagtgcc cggcgaaatc gaacacgtcg
     7921 tgaatcaaaa accgcacgtc tttgagcgat gctggcatct tggatcctcc aatgcggagg
     7981 gcacggtagc aaatcttcac ggcccgtcaa tgaccccctc cacagatcgc gttcgccccg
     8041 cgctgatgcg caaaatcgcg gcctgctccc gcccggagga gcgggtgaat cgtgacgggc
     8101 ttcgcacgcc atacgaggac attcgctact gtcgtgccaa acgtccaacg ggcaggatca
     8161 accgatgatc ggtcgcgcgt tgcgagccct gttcccgccc atctgcctgc tctgcgatca
     8221 gagctgcgca gccgaggcag acgtagacct gtgcagccgg tgcctgcccg tcctgccctg
     8281 gaacccgcag ccctgtcccc gctgcgcgct tccgcgggag aacggcaccg ggcgatgcca
     8341 accctgcgaa gacgaccctc tgccggtggc ccgcaccgtg gcgccgctgc tccacgccgg
     8401 ctacccgcgc cactgggtcc accggctcaa gtttcacgag ggcatggtcg agggccggct
     8461 gctggcgcac ctgctcgccg gggcggtccg tgaaagctac ggcaggaccg accttccgga
     8521 catgctggtg cccgttccca cggctccggg acggctggcc tggcggggtc acaatcaggc
     8581 agccaccatc gccgctttcc tgagccgccg cctgagcctg ccgctggccc ggcgcggagc
     8641 caggcgcgcc cgcagctccc ggccccagag acttctgtcc cgcgccgcac gcctgaaagc
     8701 cactgccggc atcttcgaat cacgtcagtg gcggggcgag acgatcgccg tggtcgacga
     8761 cgtcatgacc accggggcta cagtcgccga gctcgcacgc acgctgacgg cggcgggtgc
     8821 gggcgaagtc cacgtgtggt cggccacccg cgccgtctga gttccctggt agactggctc
     8881 tcatgagcga gtcgtccacc agcacaccct atgccggcct gaccccggac ctgatactcg
     8941 acgcgttgga ggccaccggg ttcgccccaa ccggaagcct gctcgagctg aacagctacg
     9001 aaaaccgggt ctatcagctg gaggttgagg acgggacgtt cgtcgtgacc aagttctacc
     9061 gtcccgagcg ctggagcgac gcgcagatcc tcgaagagca cgcgttcacc gcagagctca
     9121 tcgagcatga gctgcccgcg gtggggccgc tgcggagaga cggcaccacc ctgttcgagc
     9181 actccggatt ccgtttcgcc gtgttcccga ggcagggcgg gcaccctccc aacatagagg
     9241 tcgaggaaga tctgaaggtt ctctcccgca cgctggcccg gcttcatgcc atcggtcgga
     9301 tccgcccgtt cgagacacgc cccacgctca gctgcgagcg aatgggcagg cagagccgcg
     9361 agtttctgct ggaaacgaac ctcgttcccc tggagcttca gccggcctac gccagcacca
     9421 ccgagcatct gctcgagcgg ctggagccgc tgttcgatac cgagcaccaa tggcgccgca
     9481 tccacggcga ctgccacctg ggcaacctgc tgtggcgcgg cgaggttcca cacttcgtcg
     9541 atttcgacga ctgcgtgaac ggtccgccga tacaggacct gtggatgctg ctgtccggcg
     9601 agcaccacga gcgcgaagcc cagctgagca tgatcctgga ggcttacgat cagtttgcga
     9661 gcttcgacct gtcgagcctc aggctgatcg aacccctgcg cacgctgcga atcatgcacc
     9721 acgctgcctg gatcggccgc cgctggagcg atccggcctt tccccgtgcg ttcccgtggt
     9781 tcaacacgga gaagtactgg tcggaacaca ttctcagcct gcgagagcag atggccgctc
     9841 tcgacgagcc tacgctgacg gtaagctgag ctcccggcga ttgcgcagga cgttggcgta
     9901 cgccgccagg agatagggct tctcggcgtc ggggcacccg gcaagacgct gctcgaaggc
     9961 ggcggtcacg gcaggcagct cggccagctg cacatggcag ctggcctggc cgagccgggc
    10021 cgccgcctgc gcctgggtgg gaaagatctt caggcccgcg acgatggcgc gatcgatctc
    10081 gagggcgagg gcttccgcag tttcgaagta gccggtcacg ggcgagccga agtgcagatg
    10141 cacgcgaccc ttgaagccca tcatgccctc cacgatgctc tggaaatcct cgtccggcgg
    10201 cttgatgtag gagccttccc tttccgtcag gtaaagctcg tgggctttgc gcagatcgca
    10261 gggatcgagc tcgtaggaga cggctaccgg gacgatgcgg attcgcgtga cgagatcccc
    10321 gaagctcccc gcttccttgc ggtaggcgag acccagcatc ttcagcaggg ccggatcggt
    10381 gcgatcgaaa ccgtccttcg atcgaccctc gcgttgcgcg atccacaccg actcgccctc
    10441 ctcgagcgac tggcggatgt agtgggacgt tcgcgaaagc gcggcgtaga cggctcgggt
    10501 accgctgact ttgcgctcga ccacgaagct tttgttgagg cgcatcaggt cggcggcgaa
    10561 cggccgcgcg aacaggttgt ccccgaccgc gatgcgcgac gtctggtgcc ccgctcgatg
    10621 aatcacgtag ttgaggatgc ccgagtccat cacgatgtcc cgatgattcg acatgaacag
    10681 gtagggcttg cccttagaca gctcgtccag gccgtccacc gtcagctcca cgacggtctc
    10741 ctcgatcagg cgggccatgt actttcccag gaacagctga acgtcgttga cggtgaccag
    10801 ccccttcgtc ttgcgtctga ggtaggctct gaccaggacg ctcgccaggc ggggccagac
    10861 ccgggccaac ccgggcatca ggtagacggc caccgcgcgg tcgagttctg cgtcttcgac
    10921 caggcgcgcg accacccccg caacctccgg gtcctcgtag ggtcgaatgg cttcgaagtc
    10981 cagcatgtgc gccagttagc gccatttgac aggcagaaaa caagtcgccg cccgggcggc
    11041 ttttcctcta acatgaggag cctgctcggt gggggagaca tctgatgggc ctaggcattc
    11101 gcccctttga caagggcctg ctcgacctgg gaaacggcgc atacgcctgg ctccagcccg
    11161 acggcagctg gggttggtca aacgccggcc tggtggtcga cggggacgcc tccctgctgg
    11221 tggacacgct cttcgacaag cacctgaccc gcgagatgct cagcgccatg cgcgacgccg
    11281 cacccaaggc cacccggcac ttcgacactc tggtcaacac gcactccaac ggcgaccact
    11341 gcaacggtaa cgagctggtg accggggccg agatcgtcgc ctcgaaagcc tgcgcggagg
    11401 agctcgccgc cgagaatcca agaatgatgc agacgatgat ggagcgcgcg ccggaaatgg
    11461 gcgaagtggg cgagttcttc gttcactgtt tcggcgcttt cgacttcacc aacatcaacc
    11521 acactctgcc cacccgtacc ttcgagggag agcttggact gaccgtcggt gacaaggcgg
    11581 tgctgctcaa gcaggtcggg cccgcccaca cccgaggcga cgttctggcc tacgttcccg
    11641 atgagaagct catctacaca ggcgacatcc tcttcatcga aggccacccg attctctggg
    11701 tgggccccgt gggcaactgg atcgaagcct gcgactacat gctcggtctg gacctggaga
    11761 acgtggtgcc cggacacggt cccgtcacgg acaaacgcgg cgtgatggcc gtgcgcgact
    11821 acctgatcta cattcgtgac gaggctcgca aacgattcga tgccggcatg ccgccggacg
    11881 aggcggctgt ggacatctcg ctgacggact acgactcctg gggcgacgcc gagcggatcg
    11941 tggtcaacgt ggccgtgctc tacaaggagt tcgctgcaga tcccgagccc ggcgacatcg
    12001 ccgagctgtt cgcgctgatg gccaaggtgc acaaggcccg gcgccgttga ccgacctgcg
    12061 ccgcgccggg gaggacgatc tgcctgccct ggtccggatc tacaaccact acgtggccca
    12121 ctcccactgc acgttcgaca tcgaaaccta cagcgtcgac gagcggcgag ggtggtggcg
    12181 gcggttcgac gccgcccgat accagtgctg gatcgccgag cacgccggca gggtcatcgg
    12241 ctatgcctgc agccagccgc tgaaagccaa gggcggttac gaaacctcgg ttgaggtgag
    12301 cgtctacgtg gacgccgccc atacgggctc aggaaccgga cgctccctct accagcagct
    12361 gctcgcagcg ctcgcgcccg aggacgtgca cagggcctac gccctggtcg ccctgcccaa
    12421 cgatccctcc atcgctttgc atcaaacgat gggcttccgg gaggtggcgc acctcggcga
    12481 ggtcggccgg aagttcggcc gctactggga cgtggtctgg ctggaacgcg ccctttagcc
    12541 gagcgcggcg cgcagcagga tccagatacc catgacgagg agcacggcga acgccagccg
    12601 cttcatggcg agctcagata ccgggggctc gaaggtgcgg cccgcccagg ttcccagcac
    12661 caccacgggc aaagccagcc cggtgagcag ccacacctcc cccgtcaacc cgccattgac
    12721 ccccaccagc accgtgcgga ccgacgtcgt catggcgaag ccgcacagca gcgtggcccg
    12781 aatggccgcc acgggcagcg gttgccggta gttgaaccat cccatgatcg gacccgacgc
    12841 cgcgaacagg ccaccggcca ggccgccggt cacgcctgcg gccacgcagg cccaaggggg
    12901 tgacgtggag ggcctcggct gcgggcgcag catcatggaa aggctgccga gcacgatgaa
    12961 gacgccgagc acgacctcca gcgcccgcac ggcctggccc tccagcactt ccagaagata
    13021 gatgccagcc catatggccg gcagctgacc gaccgcgagc cagacgaaaa gacgcgcctc
    13081 cacccgctcg agatgaccgc gcagcgcgag cgccacgttc acgagagcga gcaggctgac
    13141 caccgccgcc accaccgaga cctgagaatt gccggcggcg ctggccacgg ccaccaggat
    13201 catggccatg gcgaagccgg tcacggactg cacgtagctg ccgaacaacg cgccgccaag
    13261 gaagatgccg agagtccagg ggtccatcgc ggacggcagt ataggcgatc gcggcagatc
    13321 gggtggtata gtcccggcct ccatcggggg cacgacggaa aagcaggaat ggatctcggc
    13381 ctcacaggca agacggcgct cgtcagcggc agctatcggg ggaccgggct cgtcatcgcc
    13441 cgccatctgg cagccgaggg cgtgcgggta ttcgtccacg ggctggagcc cggtcaggcg
    13501 gagcgcgccg tggaggaaat cggcagcggc gacccggtga ccggcgacat cacctgcgat
    13561 gctggcgccc gagaggtggc cgaggccctg ggcgacagcc gcgtcgacat acttgtcaac
    13621 aactacggaa ccgcggaccg gggtacctgg caggatagcg acaccgacga ctggctggac
    13681 gcctaccaga aaaacgtgct ctccgctcag cgtctgatcc gagcgtactt cgctccaatg
    13741 cgggagcggg gctggggaag gatcatcaac ctcgggaccg tgggatccac ccgacccaac
    13801 agccggatgc cccattacta cgcgtcaaag ggcgcgttgg ccaccatgac catcagcctt
    13861 gccaaggagg catcgggcac gggagtgcgg gtgaatctgg tttctcccgg cctgatcctc
    13921 acccccgagg tggaggccgc ctacctcgag cgcggccgag ccaagggctg gggagaaacc
    13981 tgggaagagg tggagccaaa cgtggcggcc gacattccca tcgggcgcat cgtccgacgt
    14041 gaggaggtgg ccgacctcgt cacgttcctg gcaagcccac tggccgacgc cattcacggc
    14101 cagaacatcc gggtggacgg cggggcgttg gacgtgctgt cttgaccgga ccaggcctga
    14161 ccagaccggg tatccggctg ctcgggctgg ccgcgctggg cctgtgtgtg ctcgcctacg
    14221 acgcgggcag ctacaccctc cttcaccgtc tcgtcctgcc catcgccatg ggcgtggctg
    14281 caggcctgat ggtgcagagt gcggccgccg ttctgctcgc cggcaccctg ctcgccggcg
    14341 ttcacgcccg tcctgcggac ccggactggg tcatagggat cgcctatccg gcgctggccg
    14401 ctctgtgtgg cgtggggctg acctggattg tcgtgctgcg tttccgccgg cggatcaaag
    14461 ccacgcgcga ggcacgctgg cgcggccgtc gcgagcgctc ggcagcgccg gaaggatcgg
    14521 agagctgaag tgggaacacg ctgcttcctg gtcagcggca ccaccagcgg catcggtctg
    14581 gccaccgcac gcgccctcgc ggccaccgga ggcaccgtcg tcacggcaaa ccgcgaccct
    14641 gacaggggcg agagcacggc cgccctgctg cgggatgcca ccagcaaccc caacgtcatc
    14701 aacaccgtgt gcaacctgtc ttcgttggca tctgtccgct cctgcgcagc ccgcatccgg
    14761 gagcagttcc cgcgcatcga cgtgctgatc aacaacgctg gcgtgtggac cgccaagcct
    14821 cagctttccg aggacggctt cgagctttct ttcgccacga atcacctcgg gccgtttctg
    14881 ctcacccaca tgctcctcga gccgctgaga gcagccggcg gcgcaaggat cgtgaacgtc
    14941 gcctcggtgc agcaccgcgc ggcggacctg gacctggatc atctgacccc aaagacgccc
    15001 tatcggggtc tgagggccta cagccggtca aagctcgcca acgtgatgac gacgctcgcc
    15061 tttgccaggc gcttcgacgc cgcggagatc tccgcaaact gcctccatcc tggcgtggtt
    15121 gcctccaaca tcgttcccga aaacagcccc ctgctgcgca gagctgcgcg tgtcgtgagc
    15181 ggcctgatgc gcagccccgc ccgcggggcc gcaacttcca tctacctggc aacgtcgcca
    15241 gaggtgacag gggtgacggg caaatacttc gaccccgacc aacgcatcat ccaaccgtcc
    15301 gcggaagccc gggacgaggc ggcacaggac ctgctctggg aacgcagcct cgagctgacc
    15361 gggctggcgg aggccggctg aaatgacacg aggcaaatcc gccatggcgt ccgcggccga
    15421 cggcatcgcc cgagcgattg ccatggggta cgaatcctat caggcggagt ttcaggccat
    15481 caccgccatg gcc
//
LOCUS       DBNHKV010000236         5062 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_386006, whole genome shotgun sequence.
ACCESSION   DBNHKV010000236 DBNHKV010000000
VERSION     DBNHKV010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_386006"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2434
                     /locus_tag="AC16PP_07615"
     CDS             <1..2434
                     /locus_tag="AC16PP_07615"
                     /inference="COORDINATES: protein motif:HMM:NF019315.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6481530.1"
                     /translation="GVYLMVGLYHLFVARRNPSMREFFWFGWMALILAAYSVETSQWK
                     YTIDLPFLVHKKIEYVVLYLGPVVMTETFARIAAVRLNIFWRVIQAVFVGYAALVLLI
                     PNIDIHYLTLTSFQYLAAIWTLGIVGLMGWHAFKGNREARSISGLMILFFFAVLTDVI
                     LDIKLLGANNLLHLMFALVVLLMAVLMANRYTATLAKLERTVEDHTADLREANLRLER
                     ASAAKSQFLANMSHELRTPMNAIIGLTHLGLKTELSDQQREYLTTVDHSAQGLLGIIE
                     SILDFSDLDGGEVTLDEQVFNIADVLERQAAIAGPAAQEKGLDLQFHGAANLPVALVG
                     DPRRLGQVLAVFIGNAIKFTDRGSIRVEVGLASATDDTASLEFSVTDSGPGMSAEEQK
                     HLFDPFSQADESHTRTHGGTGLGLAVASKLAALMDARIDVSSEPGRGSTFSLVLTLPL
                     ATDDAGDGPDSGTDAVLDLGPIRGARVLLVDDSEINLQVASEILRQIPVRVEVAHDGQ
                     EAVTMTESHHYDCVLMDVQMPVMDGYAATEAIRAGPLGEDLPILAMTANVAPEDQARA
                     KAAGMNAHIAKPIDPDRLFRELLTWIEPGEREPIEPELAVEPASLAELPDELPGIRVG
                     EGLARVGGNVDLYVSLLKDLARDYADCHTQLGELLGAGATQEAAALAHKLKGIANNLG
                     VVGVGTQAAALESALRSAEPVSESALQNLAEALAESSTAIEQLVALQSAAEGGGEESG
                     GNSLELLDRLEKEIGENDPAASDTAEELLKSLEADSAAVTAATAARDALELFDFAGAG
                     EQLDQIRAGLGS"
     gene            complement(2486..3280)
                     /locus_tag="AC16PP_07620"
     CDS             complement(2486..3280)
                     /locus_tag="AC16PP_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR6481531.1"
                     /translation="MTDLTTYYRDHWLDVEDERMARYEAMFAWREGQEVLIAPAAIGA
                     GQLVVDYGCGPGGLAVELARRVGSTGKVIGLDINAEFLERARARAADEGVAERVETRL
                     VTGDRFPVDTHAVDRVICKNVLEYVPDPQATINEFHRILRPGGIAHVSDSDWGAVVFE
                     PAREPFARIMSAAAVAFRTPLIGRSLYGMFRRAGFDDVRVQVLAAADTTGALRGVLHN
                     MAGYALTSGQVDRADVDAFISDIDRALDDKTYLAVLPQFLVTGRVP"
     gene            complement(3320..4531)
                     /locus_tag="AC16PP_07625"
     CDS             complement(3320..4531)
                     /locus_tag="AC16PP_07625"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6481532.1"
                     /translation="MATLDILPTQIDFSALHARMQWYVDERIIPHCVCLVMRGTDVLD
                     YRTLGYMDLESREPLREDAIFRMYSNTKIVTSVAAMMLFEEKRFELDDPVARFIPSFS
                     DLQVLRPGAKSAKDVEPARSPITMRQILSHSAGLSYGVFEPTSVVDQLYAQAGLHLLS
                     DTDGTLENLCEVVSRLPLAFQPGTNWRYSVSTDVTARVIEVISGQRFDVFLKERILDP
                     LGMADTDFHVPAEKQHRFTTMYAPVDLFAPMKGGLNKADDPLSGMYSRPHAMLAGGHG
                     LVSTVADYVTFLRALINGGEWHGVRLLQAETLELMRTNQLADGVSVHFPMWEMKDNVF
                     GLGFALKQAPADGEPETAVGEYHWGGIAGTHSWMAPRAGIAGVCMTQRMPGFWHPFSQ
                     EFKRMVYEQLG"
     gene            4782..>5062
                     /locus_tag="AC16PP_07630"
     CDS             4782..>5062
                     /locus_tag="AC16PP_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR6481533.1"
                     /translation="MTDSRGNFIPGLLVAIGLAVAGYLIGQGFVHARVLDRSVEVKGL
                     AEREVPADTAIWPVQIVAAGDDLQALLVQIEGQSGRVVGFLADLGFTPG"
BASE COUNT          887 a         1568 c         1680 g          927 t
ORIGIN      
        1 gggcgtctac ctgatggtcg gcctgtatca cctgttcgtt gcccggcgga acccgagcat
       61 gcgggagttc ttctggttcg gatggatggc gctgatactc gcggcgtact cggtcgagac
      121 cagccagtgg aagtacacga tcgatcttcc cttcctggtg cacaagaaga tcgagtacgt
      181 ggtgctctat ctgggaccgg tggtcatgac cgagacgttc gctcggatcg ccgctgtacg
      241 tctcaacatc ttctggcgcg tgattcaggc cgtatttgtt ggatacgcgg cgctcgtgct
      301 gctgattccc aacatcgaca tccactacct gacgctgacc tcctttcagt acctggccgc
      361 gatctggacg ctcggaatcg tcggcctgat ggggtggcac gcgttcaagg gcaatcggga
      421 agcccgttcc atatcgggct tgatgatcct gtttttcttt gccgtgctca ccgacgtcat
      481 cctggacatc aagctgctcg gtgcgaacaa cctgctccac ctcatgtttg ccctggtcgt
      541 cctcctgatg gcggtgctga tggcgaaccg gtacacggcc acgctggcaa agctggaacg
      601 aaccgtagaa gaccataccg ccgacctgcg ggaggccaat ctgcgcttgg agcgggcgtc
      661 ggcggccaaa agccagttcc tcgccaacat gagccacgag ttgcgtaccc ccatgaacgc
      721 catcatcgga ctcacccacc tggggctgaa gaccgagctt tcagaccagc agcgcgagta
      781 tctaaccacg gtcgatcatt ccgctcaggg tctgctcggc atcatcgaga gcattctcga
      841 tttctcggat ctggacggcg gcgaggtaac gctcgatgag caggtgttca acatagccga
      901 cgtgctggag cggcaggcgg ccatcgctgg ccctgcggcg caggaaaagg ggctcgacct
      961 tcagtttcat ggggcggcga accttcccgt tgcgctggtg ggcgaccccc ggcggctggg
     1021 ccaggtgctg gccgtcttca tcggcaatgc catcaagttt acggatcggg gcagcatccg
     1081 tgttgaggtc ggccttgcgt ccgcaaccga cgacaccgcc agccttgaat tcagcgtcac
     1141 ggactcgggg ccgggcatga gcgccgagga gcaaaagcat ctgttcgatc cgttttccca
     1201 ggcggacgag agccatacgc ggacccacgg aggcacaggg ttaggtctgg ctgtcgcgtc
     1261 gaagctagcg gcgctcatgg atgcgcgcat cgacgtcagc agcgaaccgg gccggggcag
     1321 cacgttttcg cttgtgctga cgctgcccct ggcaactgac gatgctggtg acgggccgga
     1381 ttcgggtacc gacgcggtgc tcgatctggg cccgattcgg ggcgcccggg tgctcctcgt
     1441 ggatgactcg gagatcaacc ttcaggtggc ctcggagatt cttcgccaga ttccggttcg
     1501 ggtggaggtg gctcacgacg gccaggaagc cgtgaccatg accgagagcc accattacga
     1561 ctgcgtgctg atggacgtgc agatgccggt gatggacggt tacgccgcca cggaagcgat
     1621 ccgtgccggg ccgctcggcg aggatctgcc gatactggcc atgacggcca acgtcgcacc
     1681 cgaggaccag gccagggcga aagccgccgg gatgaacgcg cacatcgcga aacccatcga
     1741 tcccgacagg cttttccggg agctgctgac ctggatcgag ccgggggaaa gggaacccat
     1801 tgagccggag ctggccgtcg agcccgcctc cctcgctgag cttccggacg agttgcccgg
     1861 cattcgggtc ggcgaaggcc ttgcccgcgt cggagggaac gtcgatctgt acgtctcgct
     1921 gttgaaagat ctcgccagag actatgccga ctgccacacg cagctcggcg agctgcttgg
     1981 cgctggcgca acgcaggaag cggccgcgct cgcccacaag ctcaagggca tcgccaacaa
     2041 cctcggtgtg gtaggcgtcg ggacccaggc agcggcgttg gagtctgccc tgcgatccgc
     2101 cgagcccgta tcggaatcag cgctgcagaa tctggctgaa gcgctggcag aatcctccac
     2161 ggccatcgag caactcgtgg cattgcagtc cgcggcggaa ggcggtggcg aagagtctgg
     2221 cgggaattcg ctggagctcc tggaccgcct cgaaaaggaa attggcgaga acgatccggc
     2281 agcctctgac accgccgagg aactcctcaa gagcctcgag gcggactctg ccgcggtcac
     2341 ggcggccacc gccgctcggg atgccctgga gctgttcgac tttgccggtg ccggcgagca
     2401 gctggaccag attcgcgcgg gtcttggatc ctgaattctt agcggcctca ccggctgtgc
     2461 tggcagcccg gccactcgcc gccggttacg gaacgcgacc ggttacgaga aactggggca
     2521 atacggccag gtaggttttg tcgtccagcg cgcggtcgat gtcggagatg aacgcatcga
     2581 cgtccgctct gtcgacctgg ccggatgtga gcgcgtaacc ggccatgttg tgcagaacgc
     2641 cgcgcagggc gccggtcgtg tcggcggcgg cgaggacctg aacgcgcaca tcgtcgaatc
     2701 cggcgcggcg gaacatgccg tacaggctgc ggccgatcag gggtgtccga aaagccaccg
     2761 cggctgcgga catgatccgg gcgaacggct cgcgggccgg ttcgaagacc actgcacccc
     2821 agtcgctgtc gctgacgtgg gcaatgccgc cgggccgcag aattcggtgg aactcgttga
     2881 tggtggcctg tggatccggc acgtattcga gcacgttctt gcagatcacc cgatcgaccg
     2941 cgtgcgtgtc cacggggaac ctgtcgcccg tgacgagccg ggtctcgacg cgctctgcta
     3001 ccccctcgtc tgccgcccgg gcccgggcgc gctcgaggaa ttcggcattg atgtcgagcc
     3061 cgatgacctt gcccgtgctt cccacccgac gcgccagctc tacggctagc cccccgggac
     3121 cacatccgta gtccacgacg agctggcccg cgccgatagc ggccggagcg atcagaactt
     3181 cctggccctc gcgccaggcg aacatggcct catagcgcgc catgcgctcg tcttcgacgt
     3241 cgagccagtg gtcgcggtag taggttgtca gatctgtcat ggaggaaatc ccatcaggtg
     3301 agcggcaatt gggtccttat catccgagtt gctcgtaaac catccgcttg aattcctgac
     3361 tgaaggggtg ccagaagccg ggcattcgct gggtcatgca gacgccggct atgccggccc
     3421 gcggcgccat ccaggagtgg gttcccgcga tgcctcccca gtgatactcg ccgaccgcag
     3481 tctccggttc gccgtcggcg ggcgcctgct tgagtgcaaa cccgagcccg aagacgttgt
     3541 ctttcatttc ccacatgggg aagtgcacgc tcaccccgtc agccagctgg ttggtccgca
     3601 tcagctcgag ggtttcggcc tggagcagcc gcacgccgtg ccactccccg ccgttgatca
     3661 acgcccgcag aaacgtcacg tagtccgcga cggtggagac caggccgtgg ccgccggcca
     3721 gcatggcgtg gggtctggag tacatgccgc tcagcggatc gtcggctttg ttcagccctc
     3781 ctttcatggg cgcgaacagg tcgacgggcg catacatggt cgtaaaccga tgctgcttct
     3841 cggccggcac gtggaagtcg gtatccgcca ttcccagggg atccagaatg cgctctttga
     3901 gaaaaacatc gaaacgctgg ccggagatca cttcgatgac cctggcggtg acgtcggtgc
     3961 tgacgctgta gcgccagttg gtcccgggtt gaaaggcgag cggaagccgg ctgaccacct
     4021 cacagaggtt ctcgagcgtc ccgtcggtgt cgctaaggag gtgcagacct gcctgggcgt
     4081 agagctgatc aactaccgaa gtcggctcga agacgccgta ggaaagaccg gccgaatggc
     4141 tcaggatctg ccgcatggtg atgggggatc gggcgggctc gacgtctttc gcggatttcg
     4201 cccccggccg cagaacctgc aggtcggaga acgacggtat gaatcgcgca accgggtcgt
     4261 ccagctcgaa gcgtttctcc tcgaagagca tcatggccgc cacggaggtg acgatcttgg
     4321 tgttggagta catgcggaaa atcgcgtcct cgcgcagagg ctcgcgggat tccagatcca
     4381 tgtagccgag cgtgcggtag tcgagcacgt cggtgccccg catcacgagg cagacgcagt
     4441 gagggatgat ccgctcatcg acgtaccact gcatccgggc gtgcagggca gagaagtcga
     4501 tctgcgtcgg caggatgtcg agcgttgcca tgatgtcgct cccgggcgcg gccagcggac
     4561 gattctaccg ccagccgtcc cagaatggcg tgtgttgggt ggccgggcag aatggtacgt
     4621 gcaagtaccg tcccggacgt tccgggaact cggccattac tctcgatgcg gcgtccgcgg
     4681 cagcggtatt cgtcaccgag cttgaagccg cccaccgggc tggaagcgac cccgcatgat
     4741 agtggatgat ggcgggccag cttgccgcgg atttcgtacg catgaccgat tcgaggggca
     4801 atttcattcc cgggctgctc gttgccatcg gcctcgccgt cgccggctac ctgataggtc
     4861 agggattcgt ccacgcgcgg gttttagacc gcagcgtgga ggtcaagggt ctggccgagc
     4921 gcgaggtgcc ggcagacacc gccatctggc cggtgcagat cgtggccgct ggggacgatc
     4981 tgcaagcgct tctggtgcag atcgaggggc agagcgggcg ggtcgtcggg tttctcgccg
     5041 atctcggctt cacgccgggg ga
//
LOCUS       DBNHKV010000237         3487 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_955372, whole genome shotgun sequence.
ACCESSION   DBNHKV010000237 DBNHKV010000000
VERSION     DBNHKV010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_955372"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="AC16PP_07635"
     CDS             complement(<1..336)
                     /locus_tag="AC16PP_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481534.1"
                     /translation="MSRPIIVCLVILILLPWGLRSAAAVDLRLDGLTAEQSGRAIFEA
                     ADQLEDGYADMQVDLEMILRGRGGSEVHRQLRIKQLEVPEDGDKLLVVFDTPKPIKGT
                     ALLSVTHKRA"
     gene            504..1103
                     /gene="lexA"
                     /locus_tag="AC16PP_07640"
     CDS             504..1103
                     /gene="lexA"
                     /locus_tag="AC16PP_07640"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR6481535.1"
                     /translation="MTNLTPRQAQVLDLIKQHISDTGYPPTRADIAQELGFKSPNAAE
                     EHLKALSRKGAIEMIPGTSRGIRLPEETGLPVIGRVAAGNPILAEENIEDHVDIPSGF
                     FHPRADYLLRVRGDSMIDAGILDGDLLAVHKTPEAQNGQIVVARVEDEVTVKRFQRGR
                     NRSRVTLLPENSNYAPIEVDLQAQPFAIEGLSVGVIRRS"
     gene            complement(1111..1335)
                     /locus_tag="AC16PP_07645"
     CDS             complement(1111..1335)
                     /locus_tag="AC16PP_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481536.1"
                     /translation="MVFEITQLVDGDVVLREADDEGEPLVRIRFSAEVKDMLGDDAVV
                     VAEAMIDAAAAFLEADPEDQDLTAPAPVLH"
     gene            complement(1395..>3487)
                     /gene="topA"
                     /locus_tag="AC16PP_07650"
     CDS             complement(1395..>3487)
                     /gene="topA"
                     /locus_tag="AC16PP_07650"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR6481537.1"
                     /translation="ARGLSAGRVQSVAVRLVVEREREIRAFIPEEYWEAFADLKRGAD
                     GDVLRFQLIGDGGSNFRPDNGEDAHEALGRLKDRELVVRSREDKPTRTKPGAPFITST
                     LQQAASTRLGFSVKKTMTLAQRLYEAGFITYMRTDATNLSADAVAACRDYIGEQHGAE
                     YLPDQPRTYASKEAAQEAHEAIRPSNVRTLAANLSGVDDDAVRLYDLIWRQFVACQMP
                     EARYLSTTVVVEAGELELRVRGRIMQFDGYTRVQPPSSRREEDVNLPDYAIGEKLALV
                     HAEAVQHFTKPPPRYGEASLVRELEKRGIGRPSTYAAIISTIQDRGYVTLRNRRFHAE
                     KIGELVTDRLLEKFENLMDYGFTAQMEGELDRIAEGEIGWQAVLDEFYGDFSDKLVRA
                     QSADDGMRRNEPSDTDIKCARCGRVMQVRTASTGVFLGCSGYNLPPKERCTNTMNLIP
                     GEEAVDVEQDAEAESKLLRQKLHCPRCGTAMTAYLIDEARKLHVCGNNPDCAGFVVEA
                     GQFRIKGYEGPVIECDKCGADMQLKSGRFGKYFGCSSDECSNTRKLLRNGEPAPPKAD
                     PVPMPELRCEKVDDHYLLRDGASGIFLAASQFPKNRETRAPLVREIKPHGNELDPKFN
                     YLLDAPEEDPEGRPTVIRYSRKSREQYVQSELDGKATGWKAFYRDGRWVEENQPSTRS
                     RKPAARKSSKARAG"
BASE COUNT          591 a         1177 c         1060 g          659 t
ORIGIN      
        1 ggcgcgtttg tgggtgacgc tgaggagcgc ggttcccttg atgggtttcg gggtgtcgaa
       61 aaccaccagc agcttgtctc cgtcctccgg cacttccagc tgcttgatcc gcagctggcg
      121 atgcacctcc gatcctccgc ggccgcgcag aatcatctcc agatccacct gcatgtcggc
      181 atacccgtcc tcgagctggt ctgcagcctc gaatatggct cggccggact gctcggcggt
      241 cagaccgtcg aggcgcagat ccacggccgc cgcggagcgc aagccccacg gcagaagaat
      301 gagaatgact agacagacga tgatggggcg gctcatgaac tcctcttccg ctccttcgca
      361 agaggccccc cgcggcccac ggggcttccg ggggatggac ggcggctcgg atattaagta
      421 acccccgggg accgtgcaaa ccgctcggat actgtatata ctcacagccc actgtatatt
      481 caaccaggct cgattgaccg caaatgacca acctcacccc aagacaggct caggttctcg
      541 acctcatcaa acagcacatc tcggacaccg gctacccgcc gacccgcgcg gatatcgccc
      601 aggaactggg cttcaaatcc cccaacgccg ccgaagagca tctcaaggca ctctcgcgca
      661 agggcgccat cgagatgatt cccggcacct cacgcgggat ccggctgccg gaggagacgg
      721 ggcttcccgt gataggcagg gttgcggcgg gcaacccgat tcttgccgag gagaacatcg
      781 aggaccatgt ggacattccg tccgggttct tccacccccg cgccgactat ctgctgcgcg
      841 tgcgcggcga cagcatgatc gatgcgggca ttctcgacgg ggatctgctg gcggtgcaca
      901 agacgccgga agcccagaac ggccagatcg tcgtcgcccg agtggaggac gaggtcacgg
      961 tgaagcgctt ccagcggggc cggaaccgca gcagggtgac gctgctgccg gagaattcga
     1021 actacgcgcc catcgaggtt gatctgcagg ctcagccttt cgccatcgaa gggctcagcg
     1081 tgggggtgat ccgcagaagc tgactcggcg tcagtgcaga acgggagccg gcgcggtgag
     1141 gtcctgatct tcgggatccg cctccaggaa agcggcagcc gcatcgatca tcgcctccgc
     1201 aaccaccacc gcatcatccc ccagcatgtc tttgacctcg gcagagaacc ggattcgcac
     1261 gaggggttct ccctcgtcgt ccgcctctct gaggaccacg tctccatcga cgagctgcgt
     1321 gatctcaaac accattccag attagctccc tgtgcgtctg aatttgctcg aactctgccc
     1381 gatgctcaac gcagctaacc tgcgcgggct ttgctcgact tgcgcgcggc cggcttgcgc
     1441 gaccgggtcg acggctggtt ttcctcaacc caccgaccat cgcgatagaa ggctttccag
     1501 cctgtcgctt tgccatccaa ctcggactga acgtactgct cgcggctctt gcggctgtag
     1561 cggatgacgg tcgggcgccc ttccggatcc tcttccggcg cgtcgagcag ataattgaac
     1621 ttcggatcga gctcgttccc gtggggcttg atttcccgta ccagcggcgc gcgcgtttcc
     1681 cggtttttgg gaaactgact ggcggcaaga aaaatgcccg acgctccgtc tctgagcagg
     1741 tagtggtcgt ccaccttctc gcagcgcagc tcgggcatgg gcaccggatc cgccttgggc
     1801 ggcgccggct cgccgttgcg cagcagcttg cgggtgttgc tgcactcgtc gctgctgcac
     1861 ccgaagtact tgccgaaacg gcccgacttg agctgcatgt ccgcgccgca cttgtcgcac
     1921 tcgatcaccg ggccctcgta gccctttatg cggaactggc cggcctccac cacgaagccg
     1981 gcacaatccg gattgttgcc gcacacgtgc agcttgcggg cctcgtctat caggtaggcg
     2041 gtcatggcgg tcccgcacct ggggcaatgg agcttctgcc ggagcagctt ggattcggcc
     2101 tcggcatcct gctcgacgtc cacggcctct tcgccgggaa tcaggttcat ggtgttggta
     2161 cagcgctcct tcggcggcag gttgtacccc gagcagccca gaaacacgcc cgtgctggcg
     2221 gttcgaacct gcatcacgcg cccgcagcgc gcgcacttga tgtcggtgtc tgacggctcg
     2281 ttccgacgca tgccgtcgtc cgcgctctga gcccgcacga gcttgtcgct gaaatccccg
     2341 tagaactcgt ccagcacggc ctgccagcca atctcaccct cggcaatgcg gtcgagctcc
     2401 ccttccatct gcgcggtgaa accgtagtcc atcaggtttt cgaacttctc cagcaaccgg
     2461 tcggtcacga gctcgccgat cttctcggca tggaacctgc ggttccgcag cgtcacgtac
     2521 ccgcgatcct gaatggtgga gatgatggcc gcgtaggtgg aaggccgccc gatcccacgc
     2581 ttttcgagct ctcgtaccag gctggcttcg ccgtatcgcg gcggcggctt ggtgaagtgc
     2641 tgcacggcct ccgcatgaac cagagcgagc ttctccccga tcgcgtagtc gggcagattg
     2701 acgtcctcct ccctgcgcga agacggcggt tgcaccctgg tgtagccgtc gaactgcatg
     2761 atccggccac gaacccgcag ctccagctcg ccggcctcta cgacaaccgt ggtgctgaga
     2821 tagcgggcct ccggcatctg gcacgcgacg aactgcctcc agatcaggtc gtacaggcgc
     2881 acggcgtcgt catcgactcc actgagattg gcagccagcg ttcgcacgtt ggacggccgg
     2941 atggcttcgt gagcctcctg cgcggcctcc ttgcttgcat atgtgcgcgg ctggtcgggc
     3001 agatattcgg cgccatgctg ctcgccgatg tagtcgcgac aggcggccac ggcatcggcg
     3061 ctcagattgg tggcgtccgt gcgcatgtag gtaatgaagc ctgcctcata gagcctctgg
     3121 gccagcgtca tggtcttttt cacgctgaac ccgaggcgcg tgctggccgc ctgctgaagg
     3181 gtggacgtga tgaagggcgc gccgggcttg gtgcgggtag gcttgtcctc acggctcctg
     3241 accaccagct cccggtcttt gagacgcccc agcgcctcgt gggcatcctc gccgttgtcc
     3301 ggccggaagt tgctgccgcc atcgcctatc agctggaatc tgagcacatc gccgtcagcc
     3361 ccgcgcttga gatcggcgaa agcctcccag tactcctcgg ggatgaaggc ccggatttcc
     3421 cgctcccgct ccacgaccag ccgcacggcc accgactgca cccgaccggc tgacaggccc
     3481 cgggcaa
//
LOCUS       DBNHKV010000238         2544 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1874941, whole genome shotgun sequence.
ACCESSION   DBNHKV010000238 DBNHKV010000000
VERSION     DBNHKV010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1874941"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="AC16PP_07655"
     CDS             <1..968
                     /locus_tag="AC16PP_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HIR6481538.1"
                     /translation="STDEDTLAQLALDHPIARAILDYRGMAKLKSTYTDKLPLQINQR
                     TGRIHTSYHQAVAATGRLSSSDPNLQNIPVRTPEGRRIRQAFVAPPGKRIVAADYSQI
                     ELRIMAHLSADDGLRAAFAANRDIHRATAGEVFGVGVDAVTDDQRRSAKAINFGLIYG
                     MSAFGLARQLGISRTLAQEYIDRYFERYPGVLAYMDETRARARDAGYVETVFGRRLYL
                     PEINARNMQRRQAAERAAINAPMQGTAADIIKKAMLAVDAWLAGSELDAVMIMQVHDE
                     LVFEVADADLDHLLAGVRDHMQGAADLDVELVVDCGTGANWDEAH"
     gene            complement(965..1606)
                     /gene="yihA"
                     /locus_tag="AC16PP_07660"
     CDS             complement(965..1606)
                     /gene="yihA"
                     /locus_tag="AC16PP_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR6481539.1"
                     /translation="MTETERTHKANKDRGSADRLTVQFISSAPSLRKCPRPDGPEIAL
                     AGRSNAGKSSVLNRITGSRTTAKVGKTPGRTRLLNFFAVEGGGRLVDLPGYGYAKAAR
                     SAQHEWQATVNDYLSNREALRAVVLVSDVRHPGQRFDMELLAWADASELPLLVLLNKA
                     DKLGRNAQAKALKAMQRAAEAMPLVDVILFSATSGLGNDAVVAWLREHLNAGA"
     gene            1713..2348
                     /locus_tag="AC16PP_07665"
     CDS             1713..2348
                     /locus_tag="AC16PP_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6481540.1"
                     /translation="MQIRWLPCASAIVVCLGLWAPGLAWAAGDPEAGAAQAVVCAACH
                     GQDGASGVDPSYPNLAGQNEKYLYNQLVMIQKDDRKILLMTGQLIGKSDQDLQDLAAY
                     YASLPAKLGQAEGDDDSIALAEQIYRGGIAKKGVAACAACHAPSGEGNASAGFPAVGG
                     QAGSYTIAQLTAYREGRRQTDEVYGGMMRGVAGGLTDGEIAALADYLQGLH"
BASE COUNT          415 a          811 c          864 g          454 t
ORIGIN      
        1 cgtccaccga cgaggacacg ctcgcgcaac tggcgctcga ccaccccatc gcgcgggcga
       61 ttctcgacta tcgcggcatg gccaagctga aatcgaccta taccgacaag ctgccgctgc
      121 agatcaatca gcgcacgggg cggattcaca cctcatatca tcaggcggtg gccgccacgg
      181 gccggctttc ctcgtcggat ccgaacctgc agaacattcc ggtgcgtacg cccgaaggcc
      241 gacgcatccg gcaggcgttc gtggcgccgc cgggcaaaag gatcgtagcc gccgactact
      301 ctcagatcga gctgcgcatc atggcgcatc tctcggccga cgacggcctg cgggcggctt
      361 tcgcggcgaa ccgggacatc caccgagcca cggccggtga agtattcggc gtcggcgtcg
      421 atgcggtcac cgatgatcag cgccgcagcg ccaaggccat caacttcggg cttatctacg
      481 ggatgtccgc ctttggcctc gcgcgccagc tgggcatttc ccgaaccctg gcccaggaat
      541 acatcgaccg ctacttcgag cggtatcccg gcgtcctggc ctacatggac gagacccggg
      601 cgcgggcacg cgatgcgggg tacgtggaaa ccgtcttcgg gcgccggctc tatctcccgg
      661 agatcaacgc ccgcaacatg cagcggcgtc aggctgcgga gcgcgccgcg atcaacgcgc
      721 ccatgcaggg caccgcggca gatatcatca agaaggcgat gctcgccgtg gatgcctggc
      781 tggcgggctc ggagctggat gccgtgatga tcatgcaggt ccatgacgag ctggtgttcg
      841 aggttgcgga tgctgacctg gaccatctgc tggcgggcgt ccgtgatcac atgcagggcg
      901 ctgccgacct ggatgtcgag ctggttgtgg attgcggtac cggggccaac tgggacgagg
      961 cgcactaggc tccggcgttg aggtgctcgc gcagccaggc caccaccgcg tcgttgccga
     1021 gcccgctcgt tgcggagaac aggatgacgt ccaccagcgg catggcttcg gccgcgcgct
     1081 gcatggcttt cagcgccttg gcctgagcgt tcctgcccag cttgtcggct ttgttcaaga
     1141 ggacgagcaa gggcagttcc gacgcgtccg cccacgccag gagctccatg tcgaagcgct
     1201 gcccgggatg ccggacgtcg cttacgagca ccacggcccg gagcgcttcg cggtttgaca
     1261 ggtagtcgtt cacggtagcc tgccattcgt gctgtgcgga ccgggcggcc tttgcgtaac
     1321 cgtagcctgg cagatccacc aggcgcccgc ccccttccac ggcaaagaag ttgagcagcc
     1381 tggtccggcc cggcgtcttg cccacttttg ccgtggtgcg gcttccggtg atccggttga
     1441 gcacgctgga cttgccggcg ttcgagcggc cggcgagggc gatctcgggg ccgtcgggcc
     1501 ggggacattt gcgcagcgac ggggcgctgg agatgaattg cacggtcagg cggtccgcgc
     1561 tgccgcggtc cttgttggcc ttgtgggtcc tttcggtctc cgtcaccctg ttgccctggt
     1621 ggtccttgaa gttgcattct gccagctcag cggagttgtt atataatccg cgcgccccgc
     1681 tcgggggcgg gtagtgtaaa ggaatcagag ccatgcagat tcgttggttg ccgtgcgctt
     1741 cggccatcgt tgtttgcctc gggctgtggg cgccggggct ggcgtgggcc gcgggtgatc
     1801 ccgaagcagg ggctgctcag gccgtggtct gtgccgcctg ccatggtcag gacggtgcgt
     1861 cgggtgtgga tcccagctac ccgaacctgg ccggccagaa cgagaagtat ctatacaacc
     1921 agctcgtcat gatccagaaa gacgaccgca agatccttct catgacgggc cagctcatcg
     1981 gcaagagcga ccaggacctg caggatctgg cggcatacta cgcgtcgttg cccgccaagc
     2041 tgggccaggc tgagggagac gacgactcca ttgcgctcgc cgagcagatc tatcgcggcg
     2101 ggatcgccaa gaaaggtgtg gcggcctgcg ccgcttgcca tgcgccgagc ggcgaaggca
     2161 acgcctctgc cggctttccc gccgttggcg ggcaggcggg tagttacacc atcgcccagc
     2221 tcacggccta tcgggaaggt cgccgacaga ccgacgaggt atacggcggc atgatgcgtg
     2281 gcgtggccgg cggcctgacc gatggtgaga tcgcggccct ggctgactat cttcaggggc
     2341 tgcactaagc gaaccggacg ttgtccaacg agtggttccc aaggcgccct cggttcgaat
     2401 cgcgcatggc ggggacgagg cctacccgtc gccgcagggt catggtcggg gccgaacaga
     2461 gtcctccaga gaggtagaaa tgagagacgt gctcgctttc ctgctagtgg ttgccctcgg
     2521 ggcaacgggc cccgcctttg ccca
//
LOCUS       DBNHKV010000239         2962 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_955727, whole genome shotgun sequence.
ACCESSION   DBNHKV010000239 DBNHKV010000000
VERSION     DBNHKV010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_955727"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="AC16PP_07670"
     CDS             complement(<1..355)
                     /locus_tag="AC16PP_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481541.1"
                     /translation="MPRFVLIGLCEPPNPESQQQFDEWFVDQHIEDTAKCPNFIRGSV
                     YKLSGPHLDGETVSGYLSLYEVEAPSYPEAERVLNEWQANPEAWEGRKHHLATAQKLG
                     GMPLAIKGSGWYELLK"
     gene            complement(430..1779)
                     /locus_tag="AC16PP_07675"
     CDS             complement(430..1779)
                     /locus_tag="AC16PP_07675"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6481542.1"
                     /translation="MNDIVKHPLAMDGKDPRNLRGDPITPERYYSRDYMRQEWDNLWT
                     KIWHIAGREKQLAEPGDFIVHDFMRESVIIVRQRDGSLRGFYNACGHRAQRLVWNHGH
                     QESFLCPYHGWQWGLDGSLEDLPDRDDYPQGDPVGKLGLIEVRVDTWAGLVWYTMDDE
                     APDLMKYLEPLPEIYRSFQFEKTVRSRWLRVELECNWKFWSDNFNESYHTRTAHPQVA
                     PIIDQDHFTSRYEMFPMGHARIIQMGRPSLRDRVPEGQPHPWDKELRFWGIDPDGYPD
                     YETKVMQGWLDLKAAKRTRWKEKGFHHYEHLTDEELTESPFQTVFPNIAFGASADSLG
                     VFHWEPHPTDPEKCYFDIWSMTYPVEGIEETVHRTARNPLKFEEAEYEFRVYDNGQGV
                     LDLSDQVVFQDWMLNAGQTTGWHSRGYQEPYLAAQETRVRRFHEVLHDYLAGNPPGR"
     gene            complement(1812..2132)
                     /locus_tag="AC16PP_07680"
     CDS             complement(1812..2132)
                     /locus_tag="AC16PP_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481543.1"
                     /translation="MPLHIAKYERRDHTDRTDLALAALEACRSARATAGVNSSRFYWV
                     NPDEIAIVTDAEPGAWGQGSGNQPEPRAMKAMFELADLARNTSVETWADARSGEETFK
                     ISQS"
     gene            complement(2232..2744)
                     /locus_tag="AC16PP_07685"
     CDS             complement(2232..2744)
                     /locus_tag="AC16PP_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481544.1"
                     /translation="MPALDDRSLGWLRYLHRKATTPDNWDRDGRPHPDRYRPIVPAGL
                     WGEYDVSGWTANGIEPYGMQPDPVAADGMLFYKGFFNLLMSLHRYVSGSTKWDQPFEM
                     IRDEANTFEWTHSGADLYYDPVADEHVGTGWSMQTPGRETVPLTGENNRVNVQLTTDN
                     RTVIVRRSVG"
BASE COUNT          460 a          910 c          993 g          599 t
ORIGIN      
        1 acttcagcag ctcgtaccag cccgagcctt tgatggccag cggcatgccc ccgagcttct
       61 gggcggtggc caggtggtgc ttgcgtccct cccaggcctc cggatttgcc tgccactcgt
      121 tgagcacccg ctcggcttcc gggtagctgg gggcctccac ctcgtagagc gagaggtatc
      181 cggaaacggt ctcgccgtcc aggtgaggcc cgctcagctt gtagacgctg ccccggatga
      241 agttggggca cttggcggta tcctcgatgt gctggtcgac gaaccactcg tcgaactgct
      301 gttgggattc ggggttgggc ggctcgcaga gcccgatgag aacgaatctc ggcatttgtg
      361 tgtcctgact tgtcaaatac gtaaggggga cggagcgccc tcgtgtggag agcgccgtga
      421 cccctcggct cagcgccccg gcgggttccc cgccaggtag tcgtgcagca cctcgtggaa
      481 ccgccggacc ctggtttcct gggccgccag gtacggctcc tgatagcccc tggaatgcca
      541 gcccgtggtc tggccggcgt tgagcatcca gtcctggaag accacctggt cggagaggtc
      601 gagaacgccc tggccgttgt cgtagacgcg gaattcgtac tcggcctcct cgaacttgag
      661 cgggttgcgc gccgtgcggt gcacggtctc ctcgatgccc tccaccgggt aggtcatgct
      721 ccagatgtcg aagtagcact tttccgggtc ggtcgggtgc ggctcccagt ggaagacgcc
      781 caacgaatcg gccgaggcgc cgaaggcgat gttgggaaag accgtctgga aggggctctc
      841 cgtcagttcc tcgtcggtca ggtgctcgta gtggtgaaag cctttctcct tccagcgcgt
      901 gcgcttggcg gctttgagat cgagccatcc ctgcatgacc ttggtctcgt agtcgggata
      961 gccgtcgggg tcgatgcccc agaagcgcag ctccttgtcc cacgggtgcg gctggccctc
     1021 gggaacccgg tcgcgaagcg acgggcggcc catctggatg atgcgcgcgt gccccatggg
     1081 aaacatctcg tagcgcgagg tgaagtgatc ctggtcgatg atgggtgcta cctggggatg
     1141 agcggtgcga gtgtggtagg actcgttgaa gttgtccgac cagaatttcc agttgcactc
     1201 gagctctacc cgcagccagc gggagcgtac cgtcttctcg aactggaagc tgcggtagat
     1261 ctccggaagg ggctcgagat acttcatcag gtccggcgct tcgtcgtcca tggtgtacca
     1321 gacgagcccg gcccaggtat ccacgcgcac ctcgatcagg cccagtttgc ccacggggtc
     1381 tccctgggga tagtcgtccc ggtcgggcag gtcctccaga gacccgtcaa gcccccactg
     1441 ccagccgtga taggggcaca agaaactctc ctggtggcca tggttccaca cgagccgctg
     1501 ggcgcggtgt ccgcaggcgt tgtagaaccc gcgcagggag ccgtcccgct ggcgaacgat
     1561 gatcaccgac tcgcgcatga agtcgtgaac gatgaagtcc ccgggctccg ccagctgctt
     1621 ctcgcgtccg gcgatgtgcc agattttcgt ccacaggttg tcccattcct gccgcatgta
     1681 atcgcgggag tagtagcgtt ccggcgtaat gggatcgccg cgcaggttgc gcggatcttt
     1741 tccgtccatg gcaagtggat gcttgacgat gtcgttcatc ggatatcccc tcgttttggc
     1801 tccgggtgtg atcaggattg tgagattttg aacgtctcct cgccggagcg ggcgtctgcc
     1861 caggtttcca ccgacgtgtt gcgcgccaga tcggcgagct cgaacatggc cttcatcgct
     1921 ctcggctcgg gctggttgcc ggaaccctgg ccccaggcgc ccggttcggc gtcggtgacg
     1981 atggcgatct cgtccgggtt tacccagtaa aagcggctgc tgttgacccc cgccgtggcc
     2041 cgggcgctgc ggcaggcttc cagcgcggca agtgccaggt cggtacggtc ggtatggtct
     2101 cggcgttcgt actttgcaat gtgtaatggc atggttttcc cctgatcctt tctgtcccgt
     2161 ctccggataa tggcccgaat gaaggcggtt tggaaccccg gggcgaacgc ccggcagggc
     2221 caggtcggac ctcagccgac agaccggcgc acgatcaccg tgcggttgtc ggtcgtcagc
     2281 tgaacgttca cgcgattgtt ttcgcccgtc aggggcacgg tctctcggcc tggggtttgc
     2341 atgctccagc cggtccccac gtgctcgtcg gccaccgggt cgtagtagag gtcggcgccg
     2401 gagtgcgtcc actcgaaggt gttggcctcg tcgcggatca tctcgaacgg ctgatcccac
     2461 ttcgtgctgc cggacacgta gcggtgcagg ctcatcagca ggttgaaaaa gcctttgtag
     2521 aagagcatgc cgtctgcggc cacgggatcc ggttgcatgc cgtagggctc gatgccgttg
     2581 gccgtccagc ccgacacgtc gtactccccc cacaacccgg ccgggacgat gggacggtaa
     2641 cggtccgggt ggggtcggcc gtcgcgatcc cagttgtcgg gtgtggtggc cttgcggtgc
     2701 agatagcgca gccagcctaa cgagcggtcg tccagtgcag gcatcggcag ctcccggtcc
     2761 aatgcgggta agctggcagc aatggtgcta cgctgcagcc aagcgacggg tggggaggat
     2821 gctcatgtcg gacgaaggcc gggttcccgc aacggacggg gtgcgcatga tgtttctgat
     2881 caggcgccgg gtcgggtcga gccgcgagga actggtcgcc cactggtttg ccaatcacat
     2941 gcccgccgtc atcgccgcca tg
//
LOCUS       DBNHKV010000240         4295 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_529729, whole genome shotgun sequence.
ACCESSION   DBNHKV010000240 DBNHKV010000000
VERSION     DBNHKV010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_529729"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..2015
                     /locus_tag="AC16PP_07690"
     CDS             177..2015
                     /locus_tag="AC16PP_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481545.1"
                     /translation="MGANRYALGDVMPRHSNLVLDSTSGRGFILERHPAGYASGMNSA
                     TIAGDAPVGWEVELYRNGVLLDFATVNGDGRYVFENQETVFGENVFVARLYGPQGQTA
                     EQAHTMWGGGVELESGDYDFSISHIDYDRRLLEGIDVDADRLPAEYATDLRATRALTS
                     DLQIGAAFTRAGVGNRERDGSFTDEDYLALNGRIRLARGVLIGEVVDQLDAGRAVSVE
                     YLTGRWGQSLSLARRTFRDFESPVSVRRQDVRHENEVSLSAPLGRFGLTGYSLRFRHS
                     RLSGGSDEYRIQHRLGARLGPLSLRNDLDHVVLGDSRATSGQFKAAGRFRSLSFRGQA
                     DYHLGFAETLRQVSGTLGWDISPRTYASMSVSQIVAGEGGTQFSGLFSARFGHLDLSF
                     NVSTDLEKRWKVGLGLNLSFGYDRHSRSLVTSDRSLAHSGRAALKLFVDENNNGIRDQ
                     DEQPVPWASYRDTETIGSAPGTLRLRAMPRYQPVRIETRHLKFDDPFLIPRESGYEIY
                     THAGSELSVDVAVVPTGDVEGYVFASAEDAGVSRAARDLAVSLRSPDGRIVATARTEF
                     DGFYCFTGVPPGHYQVHVGDASGRTVIESVVLDARVGLAQVDGIHL"
     gene            complement(2031..2525)
                     /locus_tag="AC16PP_07695"
     CDS             complement(2031..2525)
                     /locus_tag="AC16PP_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481546.1"
                     /translation="MAPVRAAPALTACLLGCLLVQQTAIAGPAPRPIEILEVFSPSFG
                     TWLAGPSGRQLVLFTDDTVGGADAADYLFGARSGEFVISGSANQAINILVDNTFATGG
                     IVVNQVICQYHRDLPARCDGAGVDARSGNNRTLKIGIDITTSQAHTGGDAPTINMDVA
                     IIYL"
     gene            complement(2542..3372)
                     /locus_tag="AC16PP_07700"
     CDS             complement(2542..3372)
                     /locus_tag="AC16PP_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043711 - pilus organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis chaperone"
                     /protein_id="HIR6481547.1"
                     /translation="MTTCRWVWAVLLAAAAALLSPTALAGAVLFIHPTLVVFDEDTRS
                     ARITLTNRGDETGTFECGWMELTMNPEGALRRLEDQPGWSVAPHLRFSPRRVTLKPSQ
                     SQVIKIALRPDAKLPEGEYYSHFRILTLNSEAPRGPDIESRDEAPESSGVSVQARTAV
                     AIPIVWRNGDAVMAARIQSLSLNREEKQLTVEVEREGPLSSRGFVHVILAPPGEDAEP
                     FGDPAPLVIYPNLAGRSVTLDLPDLPDGNPTGELTVVYSTEGSSERKGRVLATRSMSI
                     "
     gene            3624..>4295
                     /locus_tag="AC16PP_07705"
     CDS             3624..>4295
                     /locus_tag="AC16PP_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6481548.1"
                     /translation="MDFSFSEGDRRFQLEVREWLEEAWPEEMRARQARSALGKLTKDD
                     LVRWQKRLAERGWAATNWPVEHGGAGFTPTQNYIFDLERARIGAPGVVPFGITMVAPV
                     IMKFGTPEQKERYLPDILASNVWWCQGYSEPGAGSDLASLKTKAEDRGDHYLVNGVKT
                     WTTLAQYADMMFCLVRTSQEDIRQLGISFLLIDMSSPGISVNPIITLDTPAEGFQEIN
                     MVYFED"
BASE COUNT          788 a         1295 c         1427 g          785 t
ORIGIN      
        1 ccaccaactc cgctgagagc aggtccgcga cgctgtcaat gctgaacagc tttgatttcc
       61 tcaaacactc tgtcctctac tccggcagcc ttgccagcgg gacggaccaa gaccggtctc
      121 actcccaccg gttgacgatt gaaaggggtg caccgacggc gaacggaacc ttgttcatgg
      181 gggcgaaccg gtatgctctc ggggacgtga tgccgaggca cagcaacctc gtgctggaca
      241 gcaccagcgg gcgaggattc attctggaac ggcatcccgc gggatacgcc agcggaatga
      301 actccgccac cattgctgga gatgcaccgg tagggtggga ggtggaactc tatcgcaatg
      361 gggtcctgct cgatttcgcc acggtgaacg gggacggccg ttacgtgttc gaaaatcagg
      421 agacggtgtt cggagagaac gttttcgttg cgcgtctgta tgggccgcag ggccagactg
      481 ccgagcaggc ccacaccatg tggggtggcg gcgtggagct ggagtccgga gactacgatt
      541 tcagcatcag ccacatcgac tacgaccgcc ggctgctgga aggcatcgac gtcgacgcgg
      601 atcgacttcc ggccgagtac gcgaccgact tgcgggcgac ccgggcattg acgtccgacc
      661 tgcagattgg cgcggctttt accagggccg gcgtcggcaa tcgggagcgc gacggttcat
      721 tcaccgacga ggactacctc gcgctgaatg gccgtattcg tcttgcgcgg ggagtcctga
      781 tcggcgaggt cgtcgaccag ctcgatgcgg ggcgggccgt gagcgtcgag tacctcaccg
      841 ggcggtgggg acagagcctc tccctagctc gccggacctt ccgcgatttc gaaagccccg
      901 tttccgtgcg gcggcaggac gtacgccacg agaacgaggt gtcgttgtcc gcacccctgg
      961 ggcgtttcgg gctgacgggc tatagcctgc gcttccgtca ttctcggctg agcgggggat
     1021 ctgacgagta tcgtattcag caccgattgg gtgcccgtct gggaccgctc agcctgcgca
     1081 acgacctcga tcacgtcgtc ctgggcgaca gcagagcgac ctcgggccag ttcaaggctg
     1141 cggggcggtt tcgatcctta agcttccggg gtcaggcgga ctatcatctc ggattcgccg
     1201 agacccttcg gcaggtgtcg ggaacactcg gatgggacat ttcgccgcga acctacgcca
     1261 gcatgtcggt atcgcagata gttgcaggcg agggtgggac gcagttttcc ggactcttct
     1321 cggccaggtt cggacacctc gatctgtcgt tcaacgtcag caccgacctc gaaaagcgct
     1381 ggaaggtggg cttggggctc aacctgagtt tcggatacga ccgccacagc cgcagcctgg
     1441 tgaccagcga tcgcagcctg gcccactctg gtcgcgcggc cctgaagctc ttcgttgacg
     1501 agaacaacaa cggcattcgc gatcaggacg agcagccggt gccgtgggcg tcttatcgcg
     1561 acacggagac catcggctcg gcgccaggca cccttcgcct gcgggcaatg ccaaggtatc
     1621 agccggttcg gatcgagacg cgccacctca agttcgacga cccgttcctg atcccgcggg
     1681 aaagcggcta tgaaatctac acccacgcag ggagtgagct gagcgtggat gtggccgtgg
     1741 tgccgaccgg agatgtggag ggctacgtgt tcgcatcggc tgaggacgcg ggtgtctcca
     1801 gggcggccag agacctcgcc gtctcgctgc gatcgcctga tggcagaatc gtcgcgaccg
     1861 cgcgaaccga gttcgatggc ttctactgtt tcacgggtgt cccacccggg cactatcaag
     1921 tccacgtcgg ggatgcctca ggcagaacgg tgatcgagtc cgtggtgctc gatgccagag
     1981 ttggccttgc acaggtagac ggcatccacc tgtgagggtg cgcccgccca tcagagatag
     2041 atgatggcga cgtccatgtt gatggtcggc gcgtctccgc cggtgtgtgc ctgggacgtc
     2101 gtgatgtcta tgccgatctt cagcgttcgg ttgtttccgg atcgggcgtc gacgccggcc
     2161 ccatcgcagc gtgccggcaa gtcgcgatgg tactggcaga tgacctgatt gacgacgata
     2221 ccgccggtag cgaacgtatt gtcgacgagg atgttgatcg cctggttagc agagccggaa
     2281 atcacgaact ctccggatct tgcgccgaag agatagtcgg ccgcgtcagc gccgccaacg
     2341 gtatcgtccg tgaacagcac gagctggcga ccgctggggc ctgcaagcca ggtcccgaag
     2401 cttggcgaga aaacctcgag tatttcgatc gggcgcggcg ccggcccggc gatggccgtc
     2461 tgctgcacga gcaggcagcc cagcaggcac gcggtcagtg cgggcgcggc gcggacgggt
     2521 gccactccag cccaagcgct ctcagatgct catggaacgg gtagccagga cccggccctt
     2581 gcgctcggaa ctgccctcgg tggagtagac caccgtcagc tcccccgttg ggtttccgtc
     2641 cggcagatcc gggaggtcca gcgttaccga gcgccctgca aggttcgggt agatcacgag
     2701 aggcgccggg tcgccgaagg gctcggcgtc ttccccgggc ggcgccaata tcacatgaac
     2761 aaagccgcgg gaggaaagag gaccctcgcg ttcgacttcc accgtgagct gtttctcttc
     2821 ccggttcagg ctcaaggact ggatacgcgc ggccatgaca gcgtccccgt tgcgccatac
     2881 gatcggaatc gcgacggccg ttcgggcctg cacgctgacg cctgagcttt cgggtgcctc
     2941 gtccctgctt tcgatgtccg gcccccgtgg ggcttcgctg ttgagggtca gaatccggaa
     3001 gtgcgagtag tactcgccct cgggaagctt cgcatccggg cgcagggcaa tcttgatgac
     3061 ctgggactgc gacggtttca gagttacccg gcgcggcgag aagcgcaggt gtggagcaac
     3121 cgaccatccg ggttggtcct ccagcctgcg aagcgcgcct tccggattca tggtgagctc
     3181 catccagccg cactcgaaag tgccggtctc gtcaccgcgg ttggtcaggg taatccgggc
     3241 gctgcgggta tcttcgtcaa agacgaccag ggtagggtgg atgaacagca cggcgcctgc
     3301 gagtgccgtc ggggaaagaa gcgctgcggc ggcggcgagc agcacggccc agacccatcg
     3361 gcacgtcgtc acgctggctt tgcaggtgat cttcggcatt agcacaggga aatacgactg
     3421 atgacggcaa tataccatga gtgccggtgg tcgcgtactt cgttggggat gctgggaacc
     3481 ttgcgcattt cggtttccca cgggtatcgc cgatctccgc tacctagacg ctatctgctc
     3541 gcacgacccg tcgagggtca cgttgcgcta aaatgcgccg gccgtttgcg cggcgagctc
     3601 atcaggcagc agaagagggt catatggatt tttcattctc cgagggagat cgccggtttc
     3661 agctggaggt cagagagtgg ctcgaggagg cctggcccga ggagatgagg gcccgtcagg
     3721 ccagaagcgc tctgggcaag ctcacgaagg acgacctggt tcgctggcag aaacgcctcg
     3781 ctgaacgagg ctgggcggcg acaaactggc ccgtggagca tggcggagct ggcttcacgc
     3841 cgactcagaa ctacatattc gacctggagc gcgcacgcat cggagccccg ggagtggtgc
     3901 ccttcgggat caccatggtc gcgccggtga tcatgaagtt cggcacgccg gaacagaagg
     3961 agcgctacct gccggacata ctcgcttcca acgtctggtg gtgccagggg tactcggagc
     4021 cgggtgccgg ctcggacctc gcttcactga agacgaaagc cgaggacagg ggcgaccact
     4081 atctggtcaa cggcgtcaaa acctggacca ccctggccca gtacgcggat atgatgttct
     4141 gcctggtgcg caccagccag gaagacatca ggcagctcgg gatcagcttc ctgctgatcg
     4201 acatgtcgag ccccgggata tccgtgaacc ccatcatcac tctggacacg ccggccgagg
     4261 ggttccagga gatcaacatg gtctacttcg aggac
//
LOCUS       DBNHKV010000241        11209 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_600391, whole genome shotgun sequence.
ACCESSION   DBNHKV010000241 DBNHKV010000000
VERSION     DBNHKV010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11209)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11209)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11209
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_600391"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /gene="radA"
                     /locus_tag="AC16PP_07710"
     CDS             <1..1074
                     /gene="radA"
                     /locus_tag="AC16PP_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR6481549.1"
                     /translation="PGAGKSTLLLQVASRLAGRSGVLYVSGEESLHQLALRAERLQLA
                     ADQIRVAAETRAESVAELIAEYGPRVVIVDSIQVMQLESVDSTPGSVSQVRETAAFLT
                     RLAKQTQTAVILVGHVTKEGNLAGPKVLEHMIDCFMMLDSPAGSRYRTLRGLKNRFGA
                     VNELGVFAMTEQGMKEVANPSAIFLQRGELDSPGSVATVTWEGTRPLLVEIQALVDEV
                     QTGYAKRVAVGLDQQRLAMHLAVLHRHCGITLGDQDVFANVVGGVRIGETGADLAALL
                     AVLSSYRNRVLPKNLIVFGELGLTGEVRAVANGQQRLSEARKHGFSQAIVPFANRPKE
                     AISGMAVHGVKSLRAAMDVIDTI"
     gene            complement(1075..2757)
                     /gene="ettA"
                     /locus_tag="AC16PP_07715"
     CDS             complement(1075..2757)
                     /gene="ettA"
                     /locus_tag="AC16PP_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HIR6481550.1"
                     /translation="MAQYVYTMQGVSKTVPPKRTILENISLSFFPGAKIGVLGLNGAG
                     KSSLLRIMAGVDTEFEGEARPQKDIRVGFLPQEPKLDDSLDVRGNVEQGMGEIMSDLA
                     EYNEISDKFAEPMSDDEMAELLDRQGELATRIDAANGWDVDRTLEIAADALRLPPWDA
                     KVAHLSGGERRRVALCTLLLSKPDMLLLDEPTNHLDAESVAWLERFLAEYPGTVVAVT
                     HDRYFLDNVAGWILELDRGRGIPFEGNYSAWLEAKEKRLEMEASKEKARQRAIHSELE
                     WVRSNPKARQAKSKARLQRFEELMSKETQSRNETAELYIPPGERLGEKVIELSHVSKG
                     FDDRLLIDDLSVSIPQGAIVGIIGGNGVGKSTFFRMLTGQEAPDGGSIELGDTVELAY
                     VDQSRDALADQKTVWEEIADSQDVIRVGSYEIQSRSYVSRFNFKGPDQQKRVGDLSGG
                     ERNRVHLAKLLRRGANVLLLDEPTNDLDVETLRALEEALLNFPGTVLVISHDRWFLDR
                     VATHILAFEGDSHVEWFAGNFSEYETDHRRRLAEQGLDTEPRRVRYKPLSRG"
     gene            2904..4169
                     /gene="glyA"
                     /locus_tag="AC16PP_07720"
     CDS             2904..4169
                     /gene="glyA"
                     /locus_tag="AC16PP_07720"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003340745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0006545 - glycine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR6481551.1"
                     /translation="MFGKDQQIAGFDDEVAAAINEETRRQEEHIELIASENYASPRVM
                     EAQGGVLTNKYAEGYPRKRYYGGCEWVDKVEELAIERVKALFGAHYANVQPHSGSQAN
                     AAAYLALLEPGDTMLGMSLDAGGHLTHGAAPNFSGKVYNPVQYGLDNATGEVDYEEVA
                     RLAEEHRPRMIIGGFSAYSRRMDWQRFRDIADAVGAYLLVDMAHVAGLVAAGLYPNPV
                     PIADVTTSTTHKTLRGPRGGIILARENAEIEKKLNSALFPGTQGGPLMHVIAAKAVCF
                     KEAQGEEFVEYQRQVIANARIMAAGFQSRGYKVVSGGTDNHLFLLDLIDKEITGKAAD
                     AALGRANITVNKNAVPNDPRSPFVTSGLRIGTPAVTTRGFREAECETLTGWMCDVLDD
                     IDNDNMIGSVRQKVLDLCRRFPVYEASAA"
     gene            4204..4662
                     /gene="nrdR"
                     /locus_tag="AC16PP_07725"
     CDS             4204..4662
                     /gene="nrdR"
                     /locus_tag="AC16PP_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR6481552.1"
                     /translation="MHCPFCGADDTKVIDSRLVAEGDSVRRRRECQTCGERFTTFETA
                     ELVMPRIVKSDGTREPFNEDKLRYGLQKALEKRPVSIEEIESALNRIMHRLRASGERE
                     LPSRDVGEAVMTELRTLDQVAYVRFASVYRDFQDLSEFQAEIQKLTFDLE"
     gene            4710..5804
                     /gene="ribD"
                     /locus_tag="AC16PP_07730"
     CDS             4710..5804
                     /gene="ribD"
                     /locus_tag="AC16PP_07730"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR6481553.1"
                     /translation="MAADSDLVYLQSAVELAARGMYTCTPNPRVGCIIVRDGTVLGRG
                     WHVRAGEGHAEVNAIADAGGFVEGATVYVSLEPCAFHGRTPPCSDTLIEARVGRVVAA
                     MTDPHPEVAGRGFAALQQAGIPVEVIEMPQALELNAGYRCRIAKGRPLVRLKTAISLD
                     GRTGMASGESKWITSEAARADVQAWRAQSCAIVSGSGTIMADDASLTVRDERFRVDGA
                     IRQPLRAIADSRLELPADAAVFAGPGQTILFHGPDAEPHVPDIEHVCLGVATGADRGK
                     VDLGGMLAELGRRGCNEVLVEAGARLLGAFIRAGLWDEMIVYVAPKLLGSDARPLAHL
                     PIETMSGAVQAKMIDCEPVGDDLRIRLARA"
     gene            5823..6257
                     /gene="nusB"
                     /locus_tag="AC16PP_07735"
     CDS             5823..6257
                     /gene="nusB"
                     /locus_tag="AC16PP_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR6481554.1"
                     /translation="MNPKPNPWARRRARRALVQAVYQWQITGADAATVTAEFGKALTR
                     ADGDFFDELLRGVLSSAAELDALFAPLLDRGVNELDQVERAILRLGTYELAHRIDVPY
                     RVVIDEYVELAKLFGAEEGYKYVNGVLDGLSHLLREPERRGG"
     gene            6254..7159
                     /gene="thiL"
                     /locus_tag="AC16PP_07740"
     CDS             6254..7159
                     /gene="thiL"
                     /locus_tag="AC16PP_07740"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIR6481555.1"
                     /translation="MNEFDLIREILDVLGAATGGGAVRVGPGDDAAVIAAPEGADLVS
                     SIDAVVGDVHFPASAGASLIGYRSLMVSLSDLAAMGAEPGFALVALTLPEMDAEWTRQ
                     LASGMARAAQEASAPVVGGNIARGPLTITVSVHGWLPAGSAITRGGARPGHGVYVTGE
                     LGGAAAALTRGGLGERRVEADLDALDARYFLPAPRLEAGKRLRGLASSAIDVSDGLLQ
                     DLGHLCRASGTGAELDGASIPVMAGAKLEHALFGGDDYELCFTAAAEPGELGVACTRI
                     GTVVAGSEITLDARPVSAAGYQHFR"
     gene            7159..7647
                     /locus_tag="AC16PP_07745"
     CDS             7159..7647
                     /locus_tag="AC16PP_07745"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF016489.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A family protein"
                     /protein_id="HIR6481556.1"
                     /translation="MSTISWRDARDPAVLISCGFGSGFLPGAPGTWGSLAATALWWPL
                     LGGALWWVQAAGALLVLIAGTLLIGRVHRRHDVGDAPAIVIDEFAGMWLVLIGTGQDP
                     LLVAAAFGLFRLFDIWKPWPVRAVEARVRGGLGVMLDDVIAGAMALFVLQVSIWAIGG
                     GF"
     gene            7699..8343
                     /locus_tag="AC16PP_07750"
     CDS             7699..8343
                     /locus_tag="AC16PP_07750"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HIR6481557.1"
                     /translation="MKYLVRVLCVFSLVMPINAIASALPVEVVGLFKNHAVVRMQGGE
                     FVMKAGETRGGVTLVSADARQARMAYQGREHLVGLSERVSSQYRAVDKAQVMISSDSL
                     GQYWIRGAINNRFTNFLVDTGASVVAISAVHARQLGIEFDESHQGTVQTAQGTADTHF
                     VTLDEIVVGGITAHNVRAAIIEGEYPLEPLLGMSFLRQVSLQENAGVLTLTKQH"
     gene            complement(8352..9233)
                     /locus_tag="AC16PP_07755"
     CDS             complement(8352..9233)
                     /locus_tag="AC16PP_07755"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR6481558.1"
                     /translation="MTPELDELRALINDALDHHLPSSSAIAQPLVDAMRYTTLGGGKR
                     IRPMLVCATCTGLGGALEDALAPACAVEFIHAYSLIHDDLPSMDDDELRHGLPSSHIK
                     FGEATAVLAGDALQALAFETLSRAPSGSDAQRLRAVQLLARAAGPQGMVGGQSYDLES
                     ERKKLSLSQLQTLHAAKTGALITAAVEIGALFGKPDLSPEKHVLISEFAARIGLAFQV
                     IDDILDVTATTEKLGKPAGSDTGAGKSTFPSLIGVESARGMAEELLAEALPMLDRVGI
                     KHSLLKDLAELIVKRAF"
     gene            complement(9230..9490)
                     /locus_tag="AC16PP_07760"
     CDS             complement(9230..9490)
                     /locus_tag="AC16PP_07760"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR6481559.1"
                     /translation="MAKTSETPDDLSDELDFESALEDLESLVESMESGEMSLEESLRA
                     FERGIKLTRHCQSALKAAELKVRMLTENDELVALDADDPPPE"
     gene            complement(9560..9805)
                     /locus_tag="AC16PP_07765"
     CDS             complement(9560..9805)
                     /locus_tag="AC16PP_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481560.1"
                     /translation="MSIEQRRTKRIFRVIQEIVGAKGRDFRPGDIASVLRERGQPLGA
                     WEIRGQLSILEAEGLIRLVPESGSWEMASSENHRAVS"
     gene            complement(9860..>11209)
                     /locus_tag="AC16PP_07770"
     CDS             complement(9860..>11209)
                     /locus_tag="AC16PP_07770"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6481561.1"
                     /translation="ALAAALAAALAAALAATPAAAQAPAQEPPLDSRSFIERSVTLLK
                     EEEYALARTYLAPALIDYRLTPGERSRAYYLRGFTFFAEGFYVSAGKDYARALEFNPD
                     NPVALAAMADLYFRGLGVEADYVIAYQLYSKAAADGHANAQFHTGYLELEGIGIEKNL
                     DSARGWLHKAADQDVVPAMIHMARSYRTEATDSPDPDQARAWYERAFAAGSADALVAL
                     AFMHQDGDFGAPDPEKALELFTRAAQEGSGAAQVSLAHMHITGELLPQDYAKALELLQ
                     QAARQGVPASYLGLGHIYDTGLGVKPDRATAENWYRLAAEAELLPAQLRLVRLLLRED
                     DSTSDREAMAWLARAARQDDPQAHNDYAWLLATHEEADLRDGDLAIEFAERAVARERI
                     PSYLDTLAAAYAEAGRFEDAVSTQEQAIAMLNEAEGEIRAELAEHLDAYQAGRPWRE"
BASE COUNT         1748 a         3667 c         3822 g         1972 t
ORIGIN      
        1 cccggcgcgg gaaagagcac gctgctgctg caggttgcca gccggctggc agggcgcagc
       61 ggggttctct acgtgagcgg cgaagagtcg ctccaccagc tggcgctgcg cgccgagcgt
      121 ctgcagctgg cggcggatca gattcgcgtg gcggcggaga cccgtgcgga atcggtggcc
      181 gagctcatcg ccgagtacgg tccgcgggtc gtcatcgtgg actccattca ggtcatgcag
      241 ctggagtccg tggacagcac gccgggcagc gtatctcagg tccgcgagac cgccgccttc
      301 ctcactcgcc tcgccaagca gacccagacg gcggtcattc tcgttggcca cgtgaccaag
      361 gagggtaatc tggcggggcc caaggtcctc gagcacatga ttgattgctt catgatgctc
      421 gacagccccg ccgggagccg ttaccgcacg ctgcgcggtt tgaagaaccg gtttggagcg
      481 gtcaacgaac tgggcgtctt cgccatgacc gagcagggca tgaaggaggt cgccaacccg
      541 tcagccatct tcctgcagcg cggcgagctc gactcgcccg gcagcgtcgc cacggtcacc
      601 tgggaaggca cgcgcccact gctcgtggag atccaggctc tcgtggacga ggtgcagacc
      661 ggctacgcca agcgggtcgc cgtgggcctg gaccagcagc ggctggccat gcacctcgcc
      721 gtgctgcacc ggcactgcgg catcactctt ggcgatcagg atgtctttgc caacgtggtc
      781 ggcggcgtgc gcatcgggga gacgggcgcg gatctggcgg cgttgctggc ggtgctgtcg
      841 tcctaccgca atcgtgtgct gcccaagaac ctcatcgtgt tcggagagct cggcctgacc
      901 ggcgaggtgc gtgcggtggc caacgggcag cagcgtctgt ccgaagcccg caagcacggc
      961 ttcagccagg cgatcgtgcc gtttgcgaac cggcccaagg aggccatttc cggcatggcc
     1021 gtccacgggg tcaagagcct gcgtgccgcc atggacgtga tcgatacgat ctgatcagcc
     1081 gcgggaaagc ggcttgtagc gcacgcggcg gggctccgta tccagcccct gctcggcgag
     1141 ccgcctgcgg tggtcggttt cgtactccga gaagttcccc gcaaaccact ccacgtgcga
     1201 gtctccttcg aaagccagga tgtgggtggc cacacggtcc aggaaccagc ggtcgtggga
     1261 tatcaccagg acggttcccg gaaagttcag caacgcttcc tcgagcgcgc gcagggtctc
     1321 cacgtccagg tcgttggttg gctcgtccag cagcagcacg ttcgcgccgc ggcgcagcag
     1381 cttggccaga tgcacgcggt tgcgctcgcc gcccgagaga tcgccgaccc gcttctgctg
     1441 gtccggacct ttgaagttga accgggagac gtagctgcgc gactggatct catagcttcc
     1501 cacgcggatg acgtcctgac tgtcggctat ctcctcccat accgtcttct ggtcggccag
     1561 ggcgtcccgg ctctggtcca cgtaggccag ctccacggtg tcgcccagct cgatgctccc
     1621 gccgtccggc gcctcctgcc cggtcagcat acgaaagaag gtcgacttgc ccaccccgtt
     1681 gccgccgatg atgccgacga tggcgccctg agggatggat acgctcagat cgtcgatgag
     1741 cagccggtcg tcgaagccct tgctcacgtg ggacagctcg atgacctttt cccccagccg
     1801 ctcccctggc gggatgtaga gctcggcggt ttcgttgcgg ctctgggtct ccttggacat
     1861 gagctcctcg aagcgctgca ggcgagcctt gctcttggcc tgccgggcct tggggttcga
     1921 tctcacccac tcgagctcag agtggatcgc ccgctggcgg gctttctcct tggacgcttc
     1981 catctccagc cgtttctcct tggcttccag ccaggcgctg tagttgccct cgaacgggat
     2041 gccgcggccg cgatcgagct ccaggatcca gccggcgacg ttgtctagga aatagcggtc
     2101 gtgggtgacg gcgaccacgg tccccgggta ctcggcgagg aagcgctcca gccaggccac
     2161 gctttcggcg tcgaggtgat tggtcggctc gtccagcagg agcatgtccg gcttggagag
     2221 cagcagggtg cacagagcca cccgccgccg ctcaccgccg gagaggtggg caactttggc
     2281 atcccacggc ggcaggcgca gggcgtccgc ggcgatctcc agggtccggt ccacgtccca
     2341 gccgttggcc gcgtcgatcc tggtggcgag ctcaccctga cgatccagca gctccgccat
     2401 ctcgtcgtcg gacatgggct cggcgaactt gtcggagatc tcgttgtact cggcgaggtc
     2461 cgacatgatc tcgcccatgc cctgctcgac gttgccgcgc acgtccaggg aatcgtcgag
     2521 tttcggctcc tggggaagaa accccacccg gatgtccttc tgggggcgag cctcgccctc
     2581 gaactcggtg tcgacgccgg ccatgatcct gagcaggctc gacttgccgg ccccgttgag
     2641 gccgagcacg ccgatcttcg ccccgggaaa gaacgaaagg gagatgttct cgaggatcgt
     2701 cctcttgggt gggaccgtct tcgagacgcc ctgcatggtg tagacgtact gggccaagga
     2761 tgccgctcct ggtcgatgga ctcagagagc gcgcaagcta ccgaaatcgc cgcggtggtt
     2821 caattcaatt tccccatgaa aggattgcgt acgagatggc gccgagctgg ggtaaacttg
     2881 cgcgttttcg accgggggcg cccatgtttg gcaaagatca gcaaattgcc gggtttgacg
     2941 acgaagttgc cgcagccatc aacgaagaga cgcgacgcca ggaagagcac atcgagctca
     3001 tcgcctcgga gaactacgca agcccccggg tcatggaggc ccagggcggc gtgctgacca
     3061 acaagtacgc cgaaggctat ccccgcaagc gttactacgg cggctgcgag tgggtagaca
     3121 aggtggaaga gctggccatc gagcgggtga aagcgctgtt cggcgcgcac tacgccaacg
     3181 tccagcccca ctccggatct caggccaacg cggccgccta ccttgccctg ctcgaacccg
     3241 gcgacaccat gctgggcatg agcctggacg ccggcggcca cctcacgcac ggcgctgccc
     3301 ccaatttttc cggcaaggtc tacaacccgg tgcagtacgg cctggacaac gccacgggag
     3361 aggtggacta cgaagaggta gcccgccttg ccgaggagca ccggccccgc atgatcatcg
     3421 gcgggttttc cgcctattcc cggaggatgg actggcagag gtttcgcgac atcgccgatg
     3481 ccgtcggggc ctatctgctg gtggacatgg ctcacgtcgc cgggctcgtg gctgccggtc
     3541 tctacccgaa cccggtaccg atcgccgacg tgaccacctc caccacccac aagacgcttc
     3601 gtggaccgcg cggcggcatc atcctggccc gcgagaacgc ggagatcgaa aagaagctca
     3661 actccgcact cttcccggga acccagggcg gcccgctcat gcacgtgatc gcggccaagg
     3721 ccgtgtgctt caaggaagct cagggcgagg agttcgtgga gtaccagcgt caggtcattg
     3781 ccaatgcgcg gatcatggcc gccggcttcc agagccgcgg ctacaaggtg gtgtccggcg
     3841 gtacggacaa ccatctcttt ctgctcgacc tcatcgacaa ggaaatcacc ggcaaggcgg
     3901 ctgacgctgc cctggggcgc gccaacatca cggtcaacaa gaacgcggtg cccaacgacc
     3961 cccgctcgcc gttcgtcacc tcgggtctgc gcatcggcac cccggcggtc acgacccgcg
     4021 gcttccgcga agcggagtgc gagaccctga cggggtggat gtgcgacgtc ctcgacgaca
     4081 tcgacaacga caacatgatc ggcagcgtcc gacagaaggt gctggatctg tgccgccggt
     4141 tccccgttta cgaggccagc gcggcctgag ccgcaaggcc ccggcttcgc ctgcgcagac
     4201 gccgtgcatt gccccttctg tggtgccgac gacaccaagg tgatcgactc ccggctggtg
     4261 gctgaggggg actcggttcg ccgccggcgc gaatgccaga cctgtggcga gcgattcaca
     4321 accttcgaga ccgccgagct cgtcatgccg cgcatcgtca agagcgacgg cacccgcgag
     4381 ccgttcaacg aggacaagct gcgttacggt ctgcagaaag cgctggagaa acgtccggtc
     4441 agcatcgagg agatcgagag cgccttgaac cgcatcatgc accggctgcg ggccagcggg
     4501 gagcgtgagc ttccgtcccg ggacgtgggc gaggctgtca tgaccgagct gcgcaccctc
     4561 gatcaggtgg cctacgtgcg ttttgcttcg gtttaccgtg attttcagga cttgagcgag
     4621 tttcaggcgg agatacagaa gctcaccttc gacctggaat agcccgggcg aatagccacg
     4681 gcggacgcga acgcctatat ttgcgcgcca tggccgccga ctcggatctc gtctacctgc
     4741 aaagcgccgt ggagctggcc gcgcgaggca tgtacacgtg cacgccgaac ccacgtgtgg
     4801 gatgcatcat cgtccgtgac ggcaccgtgc tcggccgcgg ctggcacgtg cgagccggtg
     4861 aaggccacgc ggaggtgaac gccatcgccg acgccggggg cttcgtggaa ggtgccaccg
     4921 tatacgtttc cctcgagccc tgcgcttttc acggccgcac gccgccgtgc agcgacactc
     4981 tcatcgaggc gcgggtcggt cgcgtggtgg cggccatgac ggatccgcat cccgaggtcg
     5041 ccgggcgggg cttcgccgcg ctgcagcagg ctggcatacc ggttgaagtc atcgagatgc
     5101 cgcaggccct ggagctgaac gcgggctatc gctgccggat cgccaagggc cgccccctgg
     5161 tcaggctcaa gaccgccata tccctggacg ggcgcacggg aatggcgtcg ggggagagca
     5221 agtggatcac cagcgaggcg gcgcgtgccg acgtgcaggc atggcgcgcc caaagctgcg
     5281 ccatcgtcag cggctcggga accatcatgg cagacgatgc gagcctcacc gtccgcgacg
     5341 agcggttccg ggtggacggg gcgatccgtc agccgctgcg ggccatcgcc gattcccggc
     5401 tggagctgcc cgcggacgcc gccgtctttg ccgggcccgg ccagaccatc ctctttcacg
     5461 gcccggacgc cgaaccccac gtgcccgata tcgagcacgt ctgcctcggg gttgcgacgg
     5521 gagccgatcg gggcaaggtg gacctcggcg ggatgctggc ggagctcggc cgccggggct
     5581 gcaacgaggt tctggtggaa gctggcgcga ggctgctggg cgcgttcatc cgcgccggct
     5641 tgtgggacga gatgatcgtc tacgtcgcgc ccaagctgct cggcagcgat gcgcggccgc
     5701 tggctcacct tcccatcgag accatgagcg gggcggttca ggctaaaatg atcgattgcg
     5761 agccggtggg cgacgatctg aggatccgcc tggcgcgcgc ctgaacggga cccctatcag
     5821 caatgaaccc caagccgaat ccgtgggccc gccgccgcgc ccgaagggcc ctggtccaag
     5881 ccgtgtacca gtggcagatc acgggcgccg atgcggcgac ggtcacggcg gagttcggca
     5941 aagcgctcac tagggccgac ggcgacttct tcgacgagct gctgcgcggc gtgctgagct
     6001 cggcagccga gctggatgcg cttttcgcgc ccctgctgga ccggggcgtg aacgagctcg
     6061 atcaggtcga gcgcgcaatc ctgcgcctcg gcacttacga gctcgcccat cgcatcgacg
     6121 ttccctaccg cgtggtcatc gacgagtacg tcgagcttgc caagctcttt ggcgccgagg
     6181 agggctacaa gtacgtcaac ggggttctgg acggactgtc ccacctcctg agggaaccgg
     6241 agcggcgggg cgggtgaacg agttcgatct catcagagag atcctcgacg tgctgggcgc
     6301 ggccaccgga ggtggcgccg tccgcgttgg ccccggcgac gacgccgcgg tgatcgcggc
     6361 gcctgaaggc gccgatctcg tctcctccat cgacgccgtg gtcggcgacg tccactttcc
     6421 tgcttcggct ggcgcttccc tcatcggtta ccggtcgctg atggtgagcc tttcggacct
     6481 ggccgccatg ggtgcggagc cggggttcgc cctggtagcg ctgacgcttc cggagatgga
     6541 cgccgagtgg acgcggcagc ttgcgtccgg catggcccgg gcggcgcaag aggcgtccgc
     6601 gcccgtggtt ggcggcaaca tcgcgcgtgg accgctgacc atcacggtga gcgtgcacgg
     6661 ctggttaccc gcgggttcgg ccattacccg tggcggcgcc cgtcccgggc acggcgtgta
     6721 cgtcaccgga gagcttggtg gggctgcagc tgcccttacc cggggtgggc tcggtgagcg
     6781 ccgggtggaa gccgacctcg acgctctgga cgctcgctac tttctgcccg caccgcgcct
     6841 cgaggcggga aagcggctgc gcggtttggc cagcagcgcc atcgacgtgt ccgacgggct
     6901 gctgcaggac ctcgggcacc tgtgccgtgc cagcggcacc ggggccgagc tcgacggcgc
     6961 atccatcccg gtcatggccg gcgcgaagct cgagcacgcg ctctttggcg gcgacgatta
     7021 cgagctgtgc ttcaccgccg ccgcggagcc cggggagctc ggtgtcgcct gcaccaggat
     7081 cggcaccgtg gtggcggggt cggaaatcac gctggacgct cggccggttt cggccgccgg
     7141 ctaccagcac tttcgctgat gagcactatc tcctggcgcg acgcgcgcga ccccgcagta
     7201 ttgatctcct gcggcttcgg cagcggcttt ctgccgggag cgccgggtac ttggggcagc
     7261 ctggcggcga cagcgctctg gtggccgctc cttggcggtg cgctctggtg ggtccaggcc
     7321 gccggggcct tgctggtgct aatcgcgggc acgctgctca tcggccgggt gcaccgacgc
     7381 cacgacgtcg gtgatgcgcc cgccatcgtc atcgacgagt ttgccggcat gtggttggtg
     7441 ctgatcggta ccggccagga tcctctcttg gtcgccgccg ccttcggcct gtttcgcctg
     7501 ttcgacatct ggaagccctg gccggttcgt gccgtggaag cccgcgtgcg cggaggcttg
     7561 ggcgtcatgc tcgatgacgt catcgccggc gccatggcgc tttttgtgct gcaggtctca
     7621 atctgggcca ttggcggcgg gttttagcgt cgaggctgac tacactcgaa ggccgtcaag
     7681 tcgcgttcac ggacgaccat gaaatacctc gtgcgggttc tctgcgtgtt ctctctcgtc
     7741 atgccgatca acgcgatcgc ttcggctttg cccgtggagg tggtggggct gttcaagaac
     7801 cacgccgtgg ttcgcatgca gggcggcgag ttcgtgatga aagcgggcga gacccgcggc
     7861 ggggtgaccc tcgtctccgc ggatgccagg caggcgcgca tggcctatca gggccgggag
     7921 cacctcgtcg ggctgtccga acgcgtttcc agtcaatacc gcgcggtcga caaggcccag
     7981 gtgatgatca gctccgattc attgggccag tactggattc gcggcgccat caacaatcgc
     8041 ttcaccaact tcctcgtcga caccggcgcg tcggtggtcg ccatcagcgc ggtgcatgcc
     8101 cggcagctgg gcatcgagtt cgacgagtcg caccagggca cggtgcagac cgcgcagggc
     8161 actgccgaca cgcatttcgt cacgttggat gagattgtgg tcggtggcat cacggcccac
     8221 aacgtgcgcg ccgccatcat cgaaggcgag tacccgctcg agccgctgct gggcatgtcg
     8281 ttcctgcgcc aggtgagcct gcaggaaaac gcgggggtgc tcaccctcac caagcagcac
     8341 tgagcgaggg ttcagaaggc ccgcttgacg atcagctccg cgagatcctt gagcagcgag
     8401 tgcttgatac cgacccgatc cagcatgggc agcgcttcgg ccagcagctc ttcggccatc
     8461 ccgcgcgcgg actcgacgcc gatcagcgaa ggaaacgtgc tcttgcctgc tcccgtgtcg
     8521 gagccggctg gtttgccgag cttttccgtg gtggccgtga cgtccaggat gtcgtcgatg
     8581 acctggaaag cgagaccgat cctggcggcg aactcggaga tgagcacgtg tttctcgggg
     8641 gaaagatcgg gcttgccgaa aagcgcgccg atctccaccg ccgccgtgat cagcgctccg
     8701 gtcttggccg cgtgcagggt ctgcagctgg ctcaggctca gcttcttccg ctcggattcc
     8761 agatcgtagc tctgcccgcc caccatcccc tggggtccgg cggctctggc gagcagctgc
     8821 acggcgcgca gccgctgggc gtcggacccc gagggtgccc tcgacagagt ctcgaacgcc
     8881 agcgcctgca gggcgtcgcc ggcgagcacc gccgtggcct cgccgaactt gatgtggctg
     8941 ctgggcagcc cgtggcgcag ctcgtcgtcg tccattgacg gcaggtcgtc gtggatcagg
     9001 gagtaggcgt ggatgaactc caccgcgcac gccggcgcca gcgcgtcttc cagcgccccg
     9061 ccgagcccgg tgcaggttgc gcacaccagc atcgggcgga tgcgtttgcc tccgcccagg
     9121 gtggtgtaac gcatggcgtc caccaggggt tgggcgatgg cggaagaact gggaaggtgg
     9181 tgatcgagcg cgtcgttgat gagcgcgcgt agctcgtcga gttcgggagt cattccggtg
     9241 gcgggtcgtc ggcgtccagc gcaacgagct cgtcgttctc ggtgagcatg cgtaccttga
     9301 gctccgccgc tttcagcgcg gactggcagt gccgggtcag cttgatgccg cgttcgaagg
     9361 cgcgcagcga ttcctccagg gacatctcgc cggactccat gctttccacg agggattcga
     9421 ggtcctcgag cgccgattcg aaatcgagct cgtcggaaag gtcgtcgggg gtctcggagg
     9481 ttttcgccat caccaaacgg taaccggcac gggttggggg cgcaaggaaa cgatgctcgg
     9541 gcgccggtgg cgaatccgat cagctgaccg cgcggtggtt ctcggaggac gccatctccc
     9601 agctgccact ttccggtacg agccggatga gcccctctgc ctccaaaatg gacagctggc
     9661 cgcgaatttc ccacgccccg agcggctggc cgcgctcgcg cagcaccgag gcgatatcgc
     9721 caggacggaa atcgcggccc ttcgccccga cgatctcctg gatcaccctg aagattctct
     9781 tggtgcggcg ttgttcgatg ctcaaccgtt ggtcctcgaa tgctgacttt tcagccgcgc
     9841 gttttgtacc agaaacccct tattcccgcc agggtctgcc ggcctggtat gcatccaggt
     9901 gctcggcaag ctccgcgcga atctccccct ccgcttcgtt cagcatcgcg atggcctgtt
     9961 cctgggtgct gacggcgtcc tcgaatcggc cggcttcggc gtacgcggct gccagggtgt
    10021 ccagatagct tggtatgcgc tcgcgggcga cagcgcgctc ggcaaactcg atggccaggt
    10081 cgccgtcgcg aaggtcggct tcctcgtggg tagcaagcag ccacgcatag tcgttgtggg
    10141 cctgtgggtc gtcctgtcgg gcggccctgg cgagccacgc catggcttcc cggtcggagg
    10201 tgctgtcgtc ctcccggagc aggagcctga ccaggcggag ctgcgccggg agcagctcgg
    10261 cctcagccgc cagtcggtac cagttctcgg ccgtcgcgcg atcgggcttc acgcccaggc
    10321 ccgtgtcgta aatgtgcccg agccccaggt agctggccgg caccccctgc cttgccgcct
    10381 gctgaagcag ctccagggcc ttggcgtagt cctgtggcag cagctcgccg gtgatgtgca
    10441 tgtgcgccag gctgacctgg gcggccccgg agccttcctg ggcagcccgg gtgaagagct
    10501 ccagcgcctt ttcagggtcc ggcgcgccga agtccccgtc ctggtgcatg aaggccagag
    10561 ccaccagcgc gtctgcgctt cccgcggcga aagccctctc gtaccaggct cgggcctggt
    10621 ccggatccgg gctgtcggtg gcctctgtcc ggtagctgcg tgccatgtga atcatggcgg
    10681 gcaccacgtc ctgatctgcg gctttgtgca gccatcccct ggcactgtcc aggttcttct
    10741 cgatgccgat accttcgagt tccaggtaac cggtgtggaa ctgggcgttc gcgtggccgt
    10801 cggcggccgc tttggagtag agctggtagg cgatgacgta atccgcctcc acgcccaggc
    10861 cccggaagta gaggtcggcc atggcggcca gggccacggg gttgtccggg ttgaactcca
    10921 gggcccgcgc gtagtctttg cctgcggaga cgtagaagcc ctcggcgaag aaggtgaagc
    10981 cgcgcaggta gtaggcgcgg gagcgctcgc ccggtgtcag gcgatagtcg atgagtgccg
    11041 gcgcgagata ggttctggcc agcgcgtact cttcttcttt cagcagcgtt accgaccgct
    11101 ctatgaagct gcgggaatcc agcggcggct cctgggctgg cgcctgcgct gcggccggtg
    11161 tggcggcgag tgcggcggcg agtgcggcgg cgagtgcggc ggcgagtgc
//
LOCUS       DBNHKV010000242         2813 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1452047, whole genome shotgun sequence.
ACCESSION   DBNHKV010000242 DBNHKV010000000
VERSION     DBNHKV010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1452047"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="AC16PP_07775"
     CDS             <1..645
                     /locus_tag="AC16PP_07775"
                     /inference="COORDINATES: protein motif:HMM:NF039585.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HIR6481562.1"
                     /translation="LRRLTIVNRDRALLEELARLESYVKTELNVLAVSYDDDEGAHIE
                     VVAKPNFPVLGKRLGKRMKAFQSAIATLSPEQIAAFQAEGEIELDGEVFNLEEIEVLQ
                     KARPGTNTISNSRIAVDLDCVLDDELVRGGYAREIVNRIQRRRKELELNVADRIEVCF
                     AGDRALVEAAAEHRDYIAAETLATDFAEGDPGSQAADSEIDGKAFRFSVVRAGS"
     gene            complement(661..1890)
                     /locus_tag="AC16PP_07780"
     CDS             complement(661..1890)
                     /locus_tag="AC16PP_07780"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR6481563.1"
                     /translation="MWRGPITSQAVAALMLTACCTWAVTPGDPRDGYEHADYETASRQ
                     LSALRGSALALAELAGNPPLGLPPLPAERLPVAAAIDLGRRLFFDRRLSANATLSCAM
                     CHVPEQGFTQNELKTPVGIEGRFVHRNAPSLYNVAYQEALFHDGREDDLANQIWAPLL
                     AANEMGNASREAVVERLGGLEEYEGEFLRVFGDGISAATLGRALASYQTALLSADSPF
                     DRWYFGGEADAVGAEVKRGFRSFVNEGCATCHQLQTGHATFTDGAYHDTGIGFLAAAS
                     AGTPARRLQIAPGVIIDVETDIATPHVEDDGRAAVTGDPADRWRYRTPGLRNVALSAP
                     YMHDGSLATLEAVIDYYAAGGAPHDGQDARIRPLSLGADERADLVAFLQALTGSNVDA
                     LAGDARAAPIGDTHHGP"
     gene            1959..>2813
                     /locus_tag="AC16PP_07785"
     CDS             1959..>2813
                     /locus_tag="AC16PP_07785"
                     /inference="COORDINATES: protein motif:HMM:NF017505.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HIR6481564.1"
                     /translation="MKTIRKMLAQLAAGALLAMPVWADETCMSPYMAKIVGQEDFVYV
                     WTLGMEGVGDGQDKLVTVDVNPTSKTYGEVVHSLSVGGRNEAHHSGLTDDRRFLWASG
                     LDTSKIFIFDVHTNPGAPTLHRMIDDFVARSGGVVGPHTNYALPGRMMLTGLSNNRDH
                     GGRTALVEYTNAGDYIATHWMPTDDNLQGAVKSGSYADGYGYDVRVLPRRNVMITSSF
                     TGWNNYMMNLGAMMADAEAMKRFGNTVVVWDLHARQPKKILDVPGAPLEIRCAWGAKS
                     NYCFTSTAL"
BASE COUNT          485 a          901 c          957 g          470 t
ORIGIN      
        1 ctcagacgcc tgaccatcgt caaccgcgac cgagcgctgc tcgaggagct cgcccggctg
       61 gagagctatg tcaaaacgga gctcaacgtc ctggcggtga gctacgacga cgacgaaggc
      121 gcgcacatcg aggtggtcgc caagccgaac tttcccgtgc tcggcaagcg gctgggcaag
      181 cggatgaagg cctttcagag cgcgattgcg accttgtctc ccgagcagat cgccgccttt
      241 caggccgagg gcgagatcga gctcgacggg gaggtcttca acctcgagga gatcgaggtg
      301 ctgcagaaag cgcgacctgg gaccaacacc atctcaaaca gccgtatcgc cgttgatctg
      361 gactgcgttc tggacgacga gctcgtgcgc ggcggttacg cccgcgagat cgtcaaccgc
      421 attcagcgcc ggcgcaagga gctggagctg aacgtggcgg atcgcatcga ggtctgcttc
      481 gctggggatc gggctctggt cgaggcggcc gccgaacacc gtgactacat cgctgcggag
      541 actctggcca ccgactttgc cgagggcgat ccgggatccc aggcggcgga ctccgagatc
      601 gatggcaagg cgttccgctt ctccgtcgtc cgcgccggga gctgagcact cagaccgcgc
      661 tcaaggaccg tgatgcgtgt cgccgatggg cgcggccctg gcgtcgccag ccagggcgtc
      721 gacgttgctg cctgtcagcg cctgcaggaa ggcgacgagg tcggcgcgtt catcagcgcc
      781 cagagacagc ggcctgattc gggcgtcctg gccgtcgtgg ggagcgcccc ctgctgcgta
      841 gtagtcgatg accgcctcca gggtggcgag cgagccgtcg tgcatgtaag gcgcgctcag
      901 cgccacgttg cgcaaacccg gcgttcggta tcgccagcgg tcggccggat cccccgtcac
      961 ggctgctcgg ccgtcgtcct cgacgtgcgg ggtcgcgatg tccgtttcga cgtcgatgat
     1021 gaccccgggc gcgatctgca ggcggcgggc cggcgtgccc gcactggccg ctgccaggaa
     1081 gccgatgccg gtatcgtggt aggccccgtc cgtgaacgtg gcgtggcccg tctgcagctg
     1141 atggcaggtc gcgcaacctt cattgacgaa tgatcgaaat ccgcgtttga cctccgcgcc
     1201 gacggcatcg gcctcgccgc cgaagtacca gcggtcgaag ggggagtcgg ccgacagaag
     1261 agccgtctgg tagctggcca gcgcccgacc cagtgttgcg gcgctgatcc cgtcgccgaa
     1321 cacccggaga aactcgccct cgtactcctc caggcccccc aggcgctcga ccacggcctc
     1381 gcgggaggcg ttgcccatct cgttggctgc cagcagcggg gcccagatct ggttggccag
     1441 gtcgtcttcc cggccgtcgt gaaacagggc ttcctggtaa gccacgttgt agagcgaggg
     1501 tgcgttgcgg tgcacgaagc ggccttcgat gcccaccggg gttttcagct cgttctgggt
     1561 gaaaccctgc tccggcacgt gacacatggc gcaggacagc gtcgcgttgg cagacagtcg
     1621 cctgtcgaag aacagccgtc ggccgaggtc gatggccgcc gccaccggca accgctctgc
     1681 cggcaggggc ggcaagccga gaggcgggtt gcctgccagc tccgcgagcg ccagcgccga
     1741 tccgcggagt gcgctgagtt gcctcgacgc cgtctcgtag tcggcgtgct cgtacccgtc
     1801 tctcggatca ccgggcgtga cggcccaggt gcagcacgcg gtcagcatga gggcggctac
     1861 tgcctgactg gtgatcgggc ctcgccacat gtgtcttagg accgactcca ttggggtgat
     1921 aggatacctg cgagaccgtt ccagaacagg gggaaccgat gaagactatc aggaagatgc
     1981 tcgcgcagct ggcggcgggc gcgttgctcg ccatgcccgt ctgggcagac gagacgtgca
     2041 tgtcacccta catggccaag atcgtcgggc aggaagattt cgtttacgtc tggacgctcg
     2101 ggatggaggg cgtgggcgac ggtcaggaca agctcgtgac cgtggacgtc aacccgacct
     2161 cgaaaaccta tggagaggtg gtgcacagcc tgtcggtggg cggccgcaac gaggcgcatc
     2221 actcgggcct gaccgatgac cggcgctttc tctgggcgag cggtctggat accagcaaga
     2281 tcttcatctt cgacgtgcac accaaccccg gcgcccccac gctgcaccgg atgatcgacg
     2341 atttcgtggc cagaagcgga ggggtggtag gcccgcacac caactacgcg ctgccgggcc
     2401 ggatgatgct caccggcctg tctaacaatc gtgaccacgg cggccgcacg gcgctggtcg
     2461 agtacaccaa cgcgggggat tacatcgcca ctcactggat gcccacggac gacaacctgc
     2521 agggcgccgt caagtccggc agctacgccg atggttacgg ctacgacgtt cgggtgctgc
     2581 cccggcgcaa cgtgatgatc acgtcctcgt tcacggggtg gaacaactac atgatgaact
     2641 taggcgccat gatggccgat gcggaggcga tgaagcgctt cggcaacacg gtcgtcgtgt
     2701 gggatctgca tgcccgtcag ccaaagaaga ttcttgatgt tcccggcgcc ccgctggaga
     2761 ttcgctgcgc ctggggcgcc aaaagcaact actgcttcac ctccacggcg ttg
//
LOCUS       DBNHKV010000243        10920 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1946035, whole genome shotgun sequence.
ACCESSION   DBNHKV010000243 DBNHKV010000000
VERSION     DBNHKV010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10920)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10920)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10920
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1946035"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /gene="argS"
                     /locus_tag="AC16PP_07790"
     CDS             <1..1250
                     /gene="argS"
                     /locus_tag="AC16PP_07790"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR6481565.1"
                     /translation="GDWGTQFGMLLTHMTETGADSNTLSDLEDFYREAKARFDADPDF
                     AERSRQTVVRLQSGDPDTRAHWSRFIDISLSHCQAIYDRLGVKLTAADVAAESAYNDA
                     LPEVIEALRASGLLEESDGALCVFLDEFTGKDDQPLPVIVRKSDGGYLYATTDLAAIA
                     HRSRTLHADRAMYFTDARQALHFRQVFAVARTAGLAAPEMRLDHLPFGNMLGKDGTPF
                     KTREGGVVRLEELLDEAEVRAYALVGEKSPDLPETERRQIARAVGIGAIKFADLSKNR
                     TSDYVFDWDQMLSFEGNTAPYLQYAYSRIRSVFRRGDVQPDALDTPARVEMSTPEERG
                     LAVMLVRFQEVLEQVATEACPHFLCAYLYELASAFTRFYEACPILTADEPLRASRLFL
                     ADRTATTLEQGLGLLGIETMERM"
     gene            complement(1255..2460)
                     /locus_tag="AC16PP_07795"
     CDS             complement(1255..2460)
                     /locus_tag="AC16PP_07795"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DesA family fatty acid desaturase"
                     /protein_id="HIR6481566.1"
                     /translation="MSVILEWLSGGILNLSVLGMVIFTLVVTHVTIVAVTVYLHRHAA
                     HRSVEVHPALGHFFRFWLWLTTGMITREWASIHRKHHAVCETEEDPHSPKIKGLGAIL
                     WRGAEAYREASKQREIMDRYGAGCPDDWVERNVYQRFNFLGIVLMLIIDVALLGVAGI
                     TVWAVQMIWIPFFAAGVINGIGHYWGYRNFECPDAATNISPWGILIGGEELHNNHHTY
                     PNSAKLSQKPWEFDIGWFWIRLFETFGLARPLSTGPVVERVSGKTAIDVDTAWAVLND
                     RFRVMARYAEEVVAPLVEQEYHRADHATRALFKKAKKTLCREDSLVDAAGKTRIAELI
                     GHSPEIREIYEFRLRLQDVWAKRAAGADALLNELKQWCADAEATGIEVLRDFVEELKS
                     YSIPRLARA"
     gene            2677..3489
                     /locus_tag="AC16PP_07800"
     CDS             2677..3489
                     /locus_tag="AC16PP_07800"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR6481567.1"
                     /translation="MFTTLISADDLTSRLESGGDGLVVLDCRARLGEPDYGRRAYAAG
                     HLPGAVHVDLDADLAAPPGAGGRHPLPDRAELAAKLRERGLNDADQVVVYDDAGGAFA
                     SRAWWCLRWLGHRAVALLDGGLEQWPGPLSSQLPDVTPGDFSLRPPLTRTIDADRLAA
                     SLATLTLVDARTRARFEGREELIDPVAGHIPGAVCLPFGENLDGDGRFLDARTLARRF
                     EGLGADIVCYCGSGVTAAHNVLAMTIAGLDEPILYPGSWSEWIVDPTRPRAP"
     gene            3519..5276
                     /gene="mnmC2"
                     /locus_tag="AC16PP_07805"
     CDS             3519..5276
                     /gene="mnmC2"
                     /locus_tag="AC16PP_07805"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmC2"
                     /protein_id="HIR6481568.1"
                     /translation="MTWRDRQPYSAAFGDIYHDGTQAEEVNRVFVAPSRLVEKAREQR
                     SLVVGELGFGTGLNFVVAAAACLQASARLHFVSFEAAPISPPEFAALSASRAGQHPLY
                     GELGRQYPPLVRGWHRRVLSNGQITLSLYWGDAEAGLDDIDGRQLRPFDVWFLDGFAP
                     DRNPAMWTPTLLEKVGALAGDASTVATFTAAGRVRRALEAAGYNMRRVDQRPHKRESL
                     AGERRHGGRPGFAPPAHVRVAGAGLAGASVARQLAEIGIDVTVHEAGSAPGSGASGIP
                     ATVLHPRLLGDGSVQAALRCHAYLFSHPYLARWLSDPTRGVLQLPGERQGAQGIERIA
                     DRYAGTGSWLEVLPPHRAAAVADWPVASPGLWFPDGRVVDTPGLTRALLDHPRIELVT
                     GSAITTLDAPGTETTVLACGADCARFEEARYLELSPVDGQIDILASDRLPRLPLVGNG
                     YVVPGAGGLVVGTTYEYRPWPAGSATAANLRQLPEDVGRAARWLGRARGTRAVTSDRL
                     PVAGRLFDLNAHPLTNAYATTGHGSMGTVFAPFAGAVVASLISGDFPPLERDLEQALS
                     SARFRTRQARRGYRHGAQP"
     gene            complement(5282..6460)
                     /locus_tag="AC16PP_07810"
     CDS             complement(5282..6460)
                     /locus_tag="AC16PP_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR6481569.1"
                     /translation="MYKIRTLNNIAVRGLERFSREHYEVGSEIADPHAILLRSHQLAP
                     TDLNESLRAVARAGAGVNNVPVADCTEKGIVVFNTPGANANSVKELVLAALLISARDV
                     VGGIEFVSSLGDIEDEDELDRLVEAEKKRFKGRELQGKSLGVVGLGAIGSLVARAALD
                     LGMEVLGYDPAISVDAAWRLPSQVRRMENLPSLFARADYVTLHVPLLPETTGMINDES
                     LRLFRPGSVLLNFARQPIVDSGALRAALDDGRVALYVADFPVPGLTDHPNVRLTPHLG
                     ASTEEAEENCAVMAAEQLIEFLETGNIRNSVNFPSVNLEPSKGYRLAITNRNVPGMLG
                     KMTSILAESNINVIDMLNKSRDEVAYNLIDVAEEPAPELIDAICAIESVMNVREFKID
                     "
     gene            complement(6550..7617)
                     /locus_tag="AC16PP_07815"
     CDS             complement(6550..7617)
                     /locus_tag="AC16PP_07815"
                     /inference="COORDINATES: protein motif:HMM:NF009102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR6481570.1"
                     /translation="MSHVVFTGGGTAGHVVPTFPVIAALAGRGVRISFVGSNSGLEAE
                     LLEGVDADFYGIAAGKLRRYLSVENLRDVFRVLLGVWQAFRLLGRLRPDVVFSKGGFV
                     SFPVVFAAWLRRVPVVAHESDLTPGLANRLALPFLRTVCTSFSSTAFPGFKGRVVHTG
                     TPLREALLAGDRARGRQRLGAGKDERVLLITGGSLGADVINQVVREVLDELLASWLVV
                     HVCGPGKVTGTAQPRYLQFEYVTEGWGDILTAADLVVSRAGANTLYELLALGKPNLLI
                     PLSRRASRGDQLENAEFALAQGYSRVLLEEDLDGPALLREIAALDAQREAAAARLHGF
                     APPDAVELICSELTPFLPAGS"
     gene            complement(7614..8312)
                     /locus_tag="AC16PP_07820"
     CDS             complement(7614..8312)
                     /locus_tag="AC16PP_07820"
                     /inference="COORDINATES: protein motif:HMM:NF047237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdaA/DnaA family protein"
                     /protein_id="HIR6481571.1"
                     /translation="MTGPRQQLSLPFPINQRFVLGNFEIGENAEVVQRLEAEPDRSVF
                     SGLWLWGGGAVGVSHLLHGVCHWHAARGARVAYLPLGAVERDPAVLEGLDFYDLLALD
                     DVEAWAGDLQLESPLMAMYERLFARRARLIVGAAGPAARCGFRLADLASRLSSLTAYE
                     VRPLNDEGKVRLLRRLAKERGLELSDAVLGYWLARSHRSVRRLLDDLEVIDAEAMRAQ
                     RVVTIPLLKDALGL"
     gene            complement(8326..8901)
                     /locus_tag="AC16PP_07825"
     CDS             complement(8326..8901)
                     /locus_tag="AC16PP_07825"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR6481572.1"
                     /translation="MFTTAQIPNLITVARIAMVAPVGLLLWYGSVREALILMAVASAS
                     DALDGWLARHFGWMSRFGAAMDPVADKLLVGTLFVIFTLKGIIPIWVALIVLGRDVII
                     LGGALAYRLLFETIEFAPTYLSKANTAVQIVLLLLLLIAQCGFDWISGAAALLADPYG
                     FWVLAALGLTSGLDYVITWSLRAVRRAKAAL"
     gene            complement(8902..9975)
                     /locus_tag="AC16PP_07830"
     CDS             complement(8902..9975)
                     /locus_tag="AC16PP_07830"
                     /inference="COORDINATES: protein motif:HMM:NF021367.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HIR6481573.1"
                     /translation="MPTVVSARSHLLKVTLAFLLTGGAVADVVPWLYEVEVPVENQSR
                     EARLDASGEALLQVLTRVTGLTSVPRVEAVREALSAPGRYYNQFRYLEVEGVGADGRP
                     IEELKLIVQFERGSLLQLVKDAELPVWRSNRPRVAAWIVLDRGGARSILSPEDAAGLA
                     SELSARARDRGVLVSLPLMDLQDSMLVDPAAVWGRVSTVLEPASARYGADVVLVGRLQ
                     QIGAEQWTGAWDFWLDGDLHPFSMSAENAVRLVEAGVDFLADELAQRHAVLGRFARPV
                     LVGVSGVGSARDYGELLSYLAELEFVDDIAVAGVKGDRLGLVLTTRADIEQLLANFRF
                     DRRLAELPGAVPGSADVELLWRR"
     gene            10080..>10920
                     /gene="purM"
                     /locus_tag="AC16PP_07835"
     CDS             10080..>10920
                     /gene="purM"
                     /locus_tag="AC16PP_07835"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR6481574.1"
                     /translation="MTKGLTYRDAGVDIDAGNELVRRISAACKATQRPEQLTGLGGFA
                     ALAAMPERYRQPVLVTGTDGVGTKLKLAIDHDRHDQVGQDLVAMCVNDVLVTGAEPFL
                     FLDYYATGRLDVDVAERVIKGIAHGCELSGCALVGGETAEMPGFYAAGDYDLAGFCVG
                     VVEREDIVTGREIAPGDGLIGLPSSGPHSNGYSLIRRIIEERTVHVDGELLDQLMAPT
                     TIYAKQVLALAEAIPVRGMAHITGGGLLENLPRMFAAADVAALVDMDSWTRPEVFSWL
                     QQAG"
BASE COUNT         1872 a         3785 c         3561 g         1702 t
ORIGIN      
        1 tgggcgactg gggtacccag ttcggcatgc tgctcaccca catgaccgaa accggcgcgg
       61 actcgaacac cctttcggac ctggaggact tctaccggga agccaaagca cgcttcgatg
      121 ccgaccccga cttcgccgag cgcagccgcc agaccgtggt tcgtctgcag tcgggagacc
      181 cggacacccg cgcccactgg tcccggttca tcgacatttc gctgtcacac tgccaggcga
      241 tctacgaccg cctgggcgtg aagctcacgg cggccgacgt ggcagcggag agcgcctaca
      301 acgacgcgct gcccgaggtg atcgaagcgc tgcgcgcaag cggccttctc gaggagtcgg
      361 acggggcgct ttgcgtgttt ctcgacgagt tcacgggcaa ggacgatcag ccgcttcccg
      421 tgatcgtgcg aaagtcggac ggcggctatc tgtacgcgac caccgacctc gcagccatcg
      481 cgcaccgcag ccggacactg cacgcggata gggccatgta tttcaccgac gcacggcagg
      541 cgttgcactt tcgccaggtc ttcgcggttg cccgcaccgc ggggctcgcc gccccggaga
      601 tgcgcctgga ccacctgccc ttcggaaaca tgctcggcaa ggacggcacg ccgttcaaaa
      661 ccagagaagg cggcgtggta cgactcgagg agctgctcga cgaggcggag gtgcgggctt
      721 acgccctcgt gggcgagaag agtcccgatc ttccggaaac cgagcggcgt cagatcgccc
      781 gggcggtggg catcggcgcc atcaagttcg ccgacctgtc caagaaccgg acgagcgatt
      841 acgttttcga ctgggatcag atgctttctt tcgaggggaa caccgccccc taccttcagt
      901 acgcctactc acgaattcgc agcgtgttcc ggcgcggcga cgttcagccc gatgctctgg
      961 acacgccggc ccgcgtagag atgagcacgc ccgaggaacg tgggctcgcc gtcatgctgg
     1021 tgagattcca ggaagtgctc gagcaggtag cgaccgaagc ttgcccgcat ttcctgtgcg
     1081 cgtatctcta cgagctggcg agcgcgttca cccgcttcta cgaggcctgc cccatcctga
     1141 cagccgacga gccgctgcgc gcgtcgcgtc tgttcctggc ggaccggacg gccacgaccc
     1201 tcgagcaggg actcggcctg ctcgggatcg agactatgga gagaatgtga ggcgtcaggc
     1261 ccgggccagg cgcggaatcg agtaggactt caactcttcg acgaagtccc gcagcacctc
     1321 aatgccggtc gcttcggcat ccgcgcacca ctgcttgagc tcgttgagga gcgcgtcggc
     1381 accggccgcc cgcttcgccc agacatcctg caaccgcagc ctgaactcgt agatctccct
     1441 gatctcgggg ctgtgcccga tgagctcggc gatgcgcgtc ttgccggcgg cgtccacgag
     1501 agagtcctcg cggcaaagcg tcttcttggc cttcttgaac agcgcgcggg tcgcgtgatc
     1561 cgcgcggtgg tattcctgct ccaccagcgg cgcgacgact tcctcggcat agcgagccat
     1621 gacgcggaag cgatcgttga gcaccgccca ggccgtgtcg acatcgatgg cggtctttcc
     1681 gctgacccgc tctaccacag ggcccgtgct cagcggacgg gcaaggccga acgtctcgaa
     1741 gagccggatc cagaaccagc cgatgtcgaa ttcccatggc ttctgagaaa gcttggccga
     1801 attcggatag gtgtgatggt tgttgtgaag ctcctcgccg ccgatgagga tgccccaggg
     1861 cgagatgttc gtcgccgcgt cggggcactc gaaattccga tagccccagt agtgtccgat
     1921 cccgttgatc accccggccg cgaagaaggg gatccagatc atctggaccg cccacacggt
     1981 gatgccggca acgccgagca aggccacgtc gatgatcagc atcagcacga tgccgagaaa
     2041 gttgaatcgc tggtagacgt tgcgctcgac ccagtcatcc gggcaccccg cgccgtagcg
     2101 atccatgatt tcgcgctgct tggaggcttc ccgataggcc tccgcgccgc gccagagaat
     2161 ggcgccaagc cctttgatct tggggctgtg cggatcttcc tcggtctcgc agaccgcatg
     2221 gtgcttgcgg tgaatggacg cccactcgcg ggtgatcatc cccgtggtga gccacagcca
     2281 gaatcggaag aaatgcccca gagccgggtg cacctcgacg gagcggtgcg ccgcgtgccg
     2341 gtgcaggtag acggtgaccg cgacgatggt cacgtgagtg acgacgaggg tgaagatcac
     2401 catgcccagg acggacaggt tcaaaatgcc accactgagc cattccagaa tcacgctcac
     2461 gcctacagac ctccttgtgc acagctcccc cgggagcaga tcgcgcggca ttgtgacata
     2521 ggctgctcga attgccacct tgcctatcaa cgcctaccag tataagaccg ccgcaacggg
     2581 caaaggttac atccgctgcg acatgagacc tccatcgtgc taaaaggcag cctctgagag
     2641 ccagttcacg atatccaccc ggcaacgccc tgggccatgt tcaccactct gatttcggct
     2701 gacgatctga cctcccgcct ggagagcggg ggcgacggtc tcgttgtgct ggactgccgc
     2761 gcacggctgg gcgaaccgga ctacggccgc cgcgcgtacg ccgcagggca tctccccgga
     2821 gccgtgcacg tggacctgga tgcggacctg gccgccccgc caggggccgg cggccgccac
     2881 cccctgccgg accgggcgga gctggccgcg aagctcaggg agcgcggact gaacgatgcc
     2941 gatcaggtgg tggtttacga cgatgccggc ggcgcgttcg ccagccgggc ctggtggtgc
     3001 ctgcgctggt tgggccaccg cgccgtcgcc cttctggacg gcggcctcga gcagtggccc
     3061 ggaccgctct cctcccagct tcccgacgtc acgcccggag atttcagcct gcggccgccc
     3121 ctgacccgga cgatcgatgc ggaccggctc gcggcatcac tggccaccct taccctggta
     3181 gatgccagaa cgcgcgcgcg tttcgagggc cgcgaagagc tcatcgatcc ggtcgccggc
     3241 cacatccccg gcgccgtctg cctgcccttt ggtgagaacc tggacggcga cggcagattt
     3301 ctcgacgccc ggacgctggc ccggcgcttc gaagggctgg gggcagacat agtctgctac
     3361 tgcggctccg gcgtcacggc cgcccacaac gtgctggcca tgaccatcgc ggggctcgat
     3421 gagccgatcc tctatcccgg ctcctggagc gagtggatcg tcgaccccac ccggccgcgg
     3481 gcaccttgac gcgtcgggag accctagctc ctggcgttgt gacgtggcgc gaccgccagc
     3541 cctactccgc cgcgttcggc gacatctacc atgacggcac ccaggccgag gaggtcaacc
     3601 gggtcttcgt cgcgccgtcc cgactcgtcg agaaggcgag ggagcagcgg tccctggtcg
     3661 tgggcgagct cggcttcggc accggtttga acttcgtcgt ggcggcggcc gcgtgcctcc
     3721 aggcgagcgc ccgtctgcat ttcgtcagct tcgaagcagc ccccatctca cccccggagt
     3781 tcgccgcgct gagcgcgtcc cgggcaggac agcatcccct ttacggtgag ctcggccggc
     3841 agtacccgcc gctcgtccgt ggctggcacc gccgcgtgct gagcaacgga cagatcaccc
     3901 tgtctctcta ctggggcgat gccgaggcgg ggctggacga catcgacgga cgccagctgc
     3961 gtccgttcga cgtctggttt ctcgacggct ttgcccccga ccgcaacccc gccatgtgga
     4021 cgccgaccct gctcgagaag gtcggggccc tggccggtga cgcttctacg gtggcgacct
     4081 tcaccgccgc cgggcgggtg cggcgggcgc tggaagctgc cgggtacaac atgcgccggg
     4141 tggaccagcg gccacacaag cgcgaaagcc tggccggtga gcgccgccac ggcgggcggc
     4201 ccggattcgc gccaccggct cacgttcggg tggcgggagc cggtctggcc ggcgccagcg
     4261 tcgcccgaca gctggcggag atcggcatcg acgtcaccgt ccacgaggcg ggctcggccc
     4321 cgggaagcgg cgcctcgggc atccccgcga cggtgcttca cccgcggctg ctcggtgacg
     4381 gcagcgtcca ggcagccttg cgctgccacg cctatctctt cagccatcct tatctggcca
     4441 ggtggctttc cgatccgacg cgaggcgttc tgcagcttcc cggggagcga cagggcgccc
     4501 aaggcatcga gcgcatcgcc gatcgctatg cgggcaccgg aagctggctg gaagtcctgc
     4561 cgccgcacag agcggccgcg gtcgccgact ggccggtggc ttcaccgggc ctgtggttcc
     4621 ctgatggcag ggtcgtggac acgccggggc tgacccgggc gctgctggac cacccccgga
     4681 tcgagctcgt gacgggttcg gcgatcacca cgttggatgc gcccgggacc gagaccacgg
     4741 tgctcgcctg cggggccgac tgcgcacggt tcgaagaagc ccggtacctg gagttatcgc
     4801 ccgtcgacgg ccagatcgac atcctggcat ccgaccgcct tcccaggttg cctttggtgg
     4861 gcaacggcta cgtggtgccc ggcgccggcg ggctcgtcgt gggcacgacg tacgagtacc
     4921 ggccctggcc ggccggttcc gccaccgcag caaacctgcg tcagctgccg gaggacgtcg
     4981 ggcgcgcggc ccgctggctg ggtcgtgcgc gaggaacccg cgcggtgact tcggaccggc
     5041 tgccggtggc cgggcgcctg ttcgacctga acgcgcaccc cctgacgaac gcgtacgcga
     5101 ccaccggtca tggctccatg gggacggtgt tcgcgccttt cgccggcgcc gtcgtcgcct
     5161 cgctgatcag cggcgacttc cccccgctgg agcgggatct ggagcaggcc ctgtcgtccg
     5221 cgcggttccg cacgcgccag gcgcggcgtg gctatcggca cggggcgcag ccttaggccg
     5281 gctagtcgat cttgaactcg cgcacgttca tgacgctctc gatggcgcag atggcatcga
     5341 tgagctcggg cgccggctcc tcggcaacgt cgatcaggtt gtaggccact tcgtcccggc
     5401 tcttgttgag catgtcgatg acgttgatgt tcgactccgc caggatcgac gtcatcttgc
     5461 ccagcatgcc cggcacgttg cggttcgtga tcgccagccg atagcccttg ctgggctcca
     5521 gattcacgga cgggaagttg accgagttgc ggatgttgcc ggtttccaga aactcgatga
     5581 gctgctccgc ggccatcacc gcgcagttct cctctgcctc ctcggtgctc gccccgagat
     5641 gaggggtgag cctgacgttg gggtggtcgg tcagccccgg gaccgggaaa tcggccacgt
     5701 agagggcgac ccggccatca tccagcgctg cgcgcagggc ccccgagtca acgatgggct
     5761 gtcgggcaaa gttcagcagc acgcttcccg gccggaagag cctgaggctc tcgtcgttga
     5821 tcatccccgt ggtttccgga agcagcggaa cgtgcagggt cacgtagtcg gcccgcgcaa
     5881 acaggctcgg caaattctcc attcggcgga cctggcttgg cagacgccag gcggcatcga
     5941 ccgagatggc cgggtcgtag cccagcacct ccatgccgag atcgagggcc gcacgagcca
     6001 ccagcgagcc gatggcgcca agccccacga cgccgagtga cttgccctgc agctcgcgcc
     6061 ccttgaaccg ctttttctcg gcttcgacaa gccgatcgag ctcgtcttcg tcctcgatat
     6121 cgcccagcga gctgacgaac tcgatgccgc ccaccacgtc ccgcgcggag atcagcagag
     6181 cggccagaac cagctccttg accgagttcg cgttggcacc gggcgtgttg aacaccacga
     6241 tgcccttttc cgtgcagtcc gccaccggga cgttattgac cccggcaccc gcccgcgcta
     6301 ccgcgcgcag gctctcgttg aggtcggtcg gggccagctg gtggctgcgc agaagaatgg
     6361 cgtgcgggtc cgcgatctcc gagcccacct cgtagtgctc gcgggaaaat ctttccagcc
     6421 cgcgaacggc aatgttgttt aatgtccgaa tcttgtacat acgctgcagc cgctgtcccg
     6481 ttgcccatca cccccggagg cggggggcgc gtatggtata caaatcctgt tgccgcgcgg
     6541 aaggtccgat caggatccgg ccggaagaaa gggggtcagc tccgagcaga tcagctccac
     6601 cgcgtcaggc ggcgcaaagc catgcagccg ggccgccgcg gcttcacgtt gcgcatccag
     6661 cgcggcgatc tctcgcagca gcgcggggcc gtccagatct tcctccagca gcacccggct
     6721 gtaaccctgg gccagggcaa actcggcgtt ctccagctga tcgcccctgc tggcccgccg
     6781 ggaaaggggg atcagcagat tcggcttgcc gagagccagc agctcataga gcgtgttggc
     6841 ccccgcacgg gagaccacca ggtctgcggc cgtcaggatg tcgccccagc cctcggtgac
     6901 gtactcgaac tgcaggtagc gcggttgcgc tgtgcccgtg acctttcccg gcccacacac
     6961 gtggaccacc agccagctcg ccagcagctc gtcgagcacc tcgcgaacca cctgattgat
     7021 gacgtccgcg ccgaggctgc ccccggtgat cagcagcacg cgctcgtctt ttcccgcccc
     7081 gagccgctgc cggccccggg cgcggtcccc ggccaggagc gcttcccgca ggggtgtccc
     7141 cgtatgcacc acccgccctt tgaagccggg aaacgccgtc gaggaaaagc tggtgcagac
     7201 ggttctaagg aacggcaggg ccaggcggtt ggccagaccg ggcgtgaggt cggattcgtg
     7261 ggcaacgacg ggcacgcgcc gcagccaggc ggcgaaaacc actggaaaac tgacgaagcc
     7321 gcctttggag aacaccacgt cgggacgcag tcggccgagc agcctgaatg cctgccagac
     7381 ccccagcagc acgcgaaaga cgtcgcgaag attctcgaca gacaggtagc ggcgcagctt
     7441 gcccgcggcg atgccgtaga agtcggcatc gacgccttcg agcagttccg cttccagccc
     7501 gctgttgctg ccaacgaagc ttatccgaac gccgcggccg gcaagcgcgg cgatcacggg
     7561 gaaggtgggc accacgtgac cggccgttcc gccaccggtg aagaccacgt ggctcacagg
     7621 cccagcgcgt ccttgagcag gggaatggtc acgaccctct gagcgcgcat ggcctccgca
     7681 tcgatgactt ccaggtcatc gagcaggcgc cgaaccgagc ggtggctgcg cgcaagccag
     7741 tagccgagca ccgcatcgga cagctccagc ccccgctcct tggccaaccg ccgcaggaga
     7801 cgtaccttgc cctcgtcgtt caggggcctg acctcgtatg cggtcaggct cgacaggcgc
     7861 gaagcgaggt cggcgagccg gaagccgcat cgagctgccg gacccgccgc cccgacgatc
     7921 aacctggctc gccgcgcgaa cagccgctcg tacatcgcca tcagcgggct ctcgagctgc
     7981 agatcaccgg cccaggcctc gacgtcgtcg agggccagaa gatcatagaa atccagtcct
     8041 tccaggacgg cgggatcacg ctcgacggcc cccaagggca gataggcaac ccgcgcgccg
     8101 cgcgccgcgt gccaatgaca cacgccatgc agcagatggc tgacacccac cgccccgccg
     8161 ccccagagcc atagacccga gaagacgctc cgatccggct ccgcctcgag tcgctggacg
     8221 acctcggcat tttctccgat ctcgaagttg cccagcacga atcgctgatt gatgggaaac
     8281 ggcagcgaca gctgctgtcg cggacccgtc atcgtcccgg ccgactcaca gcgcggcctt
     8341 ggcccggcgc acggctcgca ggctccaagt gatcacgtag tccagccccg acgtgagccc
     8401 gagggcggca aggacccaga agccgtacgg atcggccagc agcgccgccg ccccgctgat
     8461 ccagtcgaag ccgcattgcg cgatgagcag cagcagaagc aggacgatct gcacggcggt
     8521 attcgctttg ctgaggtagg tgggggcaaa ttcgatggtc tcgaaaagca ggcggtaggc
     8581 aagggccccg cccaggatga tcacgtcgcg gcccaggacg atcagggcaa cccagatcgg
     8641 tatgatcccc ttgagggtga agatcacgaa cagcgtgccc accagcagct tgtccgcaac
     8701 cggatccatg gcggcaccaa agcggctcat ccaaccgaag tgtcgggcca gccatccgtc
     8761 cagcgcatcg gacgcgctgg cgaccgccat cagaatcagc gcctcccgga ccgacccgta
     8821 ccagagcaac aggccgacgg gagcgaccat cgcgatgcgc gccacggtga tgaggttggg
     8881 gatctgggcc gtcgtgaaca ttcagcgtcg ccagagcagc tcgacgtccg ccgagcccgg
     8941 caccgcgccc ggcagctcgg cgagccgccg gtcgaagcga aagttcgcaa gcagctgttc
     9001 gatgtcggcg cgagtggtga gcaccagtcc gaggcgatca cccttgacgc cggccactgc
     9061 gatgtcgtcg acgaattcca gctctgccag atagctgagc agctccccgt agtctctggc
     9121 cgagccgacg ccggacacgc cgacgagcac cggccgggca aacctgccga ggacggcatg
     9181 ccgctgcgcg agctcgtcgg caagaaagtc cacgcccgcc tccaccagcc ggacggcatt
     9241 ttcggcactc atcgaaaacg gatgcagatc gccatcgagc cagaaatccc atgcgcccgt
     9301 ccactgctcg gcgccgatct gctgcagacg cccgacgagg acgacgtccg cgccgtagcg
     9361 ggccgaggcc ggctcgagca ccgtagatac ccgcccccaa accgcggcgg gatccacgag
     9421 catactgtcc tgaaggtcca tcagcggcag cgacaccagc accccccggt cccgggcccg
     9481 cgccgacagc tcggaagcga ggccggccgc atcttccgga gagaggatgg aacgtgcccc
     9541 gccgcggtcc agcacgatcc aggccgccac ccgaggacgg ttggaacgcc agacgggcag
     9601 ctcggcatcc ttgaccagct gcagcagaga tccgcgctcg aactgcacga tgagcttgag
     9661 ctcttcgatc ggccgtccgt ccgcgcccac accctccacc tcgaggtagc ggaactggtt
     9721 gtagtagcgc cccggagccg acagcgcttc ccgaaccgcc tccacgcggg gcacgctggt
     9781 cagacccgtc acgcgggtga gcacctgcag cagggcttcg ccggaggcgt cgagccttgc
     9841 ctcgcggctc tggttctcca ccgggacctc tacctcgtag agccagggaa cgacgtccgc
     9901 gacggcgccg cccgtcagca ggaacgcaag cgtgactttc aggaggtgcg atcgtgctga
     9961 gaccaccgtg ggcaccgcgg ctccgacatc agtacggcgt cgaagtttac ccaattcgcc
    10021 aggtcaggtc tggtaaactc cgccgccatc aaggggcggc aacagggcga ggattcaacg
    10081 tgaccaaggg tctcacctac cgtgacgccg gggtggacat cgacgccggc aacgagctcg
    10141 tacgccgaat ctcagccgcc tgcaaagcga cgcagcgacc cgagcagctc accggcctcg
    10201 gcggcttcgc ggccctggcc gcgatgcccg agcgttaccg gcaaccggtc ctggtgacgg
    10261 gcaccgacgg tgtgggcacc aagctcaagc tcgccatcga tcacgaccgg cacgaccagg
    10321 tcggccagga cctggtggcc atgtgcgtga acgacgttct ggtgaccggt gccgagccgt
    10381 ttcttttcct ggactactac gccaccggcc gactggacgt ggacgtggcg gaacgggtca
    10441 tcaaaggtat cgcccatggc tgcgagctgt ccggctgcgc cctggtgggc ggcgagaccg
    10501 ccgaaatgcc cgggttttac gcggccggcg actacgacct ggccgggttc tgcgtcgggg
    10561 tcgtcgagcg cgaggacatc gtcaccggca gagagatcgc gcctggcgac gggttgatcg
    10621 ggctgccgag cagcggcccc cattccaatg gatactcgct catccgtcgg atcatcgagg
    10681 agcgaaccgt ccacgtggat ggtgagctgc tagaccagct catggcgccg accaccatct
    10741 acgccaagca ggtgctcgcg ctggcagaag ccatccccgt gcgcggcatg gcccacatca
    10801 ccggcggcgg ccttctcgag aacctgcccc gaatgttcgc cgccgccgac gttgcggcgc
    10861 tggtggacat ggactcgtgg acccggccgg aggtgttcag ctggctgcag caggccggca
//
LOCUS       DBNHKV010000244         3553 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1804828, whole genome shotgun sequence.
ACCESSION   DBNHKV010000244 DBNHKV010000000
VERSION     DBNHKV010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1804828"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..816
                     /locus_tag="AC16PP_07840"
     CDS             133..816
                     /locus_tag="AC16PP_07840"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR6481575.1"
                     /translation="MATRANNNAEQQGCAPAISLTEQIAEHLAREIITGRLGANARIQ
                     ELRVADDLGVSRGSVREALLILESRHLIDLLPRRGAIVRDLTGEELVSFSELFTDLAA
                     AFFGKLARVPDLDLGLPAQAVEEMAMALGRNDLEGSIAARKAFVDGCLAELNDVYLVS
                     VLRALLPSALRLAHRAARHTGYEMRDTIRFHHALLEALSRGDDNRVGELVRAFGRREG
                     QLAAECRSS"
     gene            902..>3553
                     /gene="smc"
                     /locus_tag="AC16PP_07845"
     CDS             902..>3553
                     /gene="smc"
                     /locus_tag="AC16PP_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR6481576.1"
                     /translation="MRLKQIKLAGFKSFVDPTTVILPSNRCAVVGPNGCGKSNIIDAV
                     RWVMGESSAKQLRGENLTDVIFNGSNSRKPTAIASIELIFSNEDGRIGGEYAAYAEIS
                     VRRQVTREAQSTYFLNGNKCRRRDIMDIFLGTGFGPRSYSIIEQGMISQLVEAKPEDL
                     RVYLEEAAGISKYKERRRETENRVRHTRENLDRLNDIREELDRQLQHLKRQSRAAERY
                     RELKEEERKLTAELHTIRLVALEAVLDAHRERIQQLEVELSRRQAEQQAIDTQIEQGR
                     QAHADTSAAVNQVQGTFYQLGADIARIEEAIQFNQQRVKQLELDLETVTRRSEETGRQ
                     LAMDETQIRELREQLAELEPRRQEAERGDGLAAARLAELEQKHRAWQAEWDEFRNRAS
                     QNERDAEVQASRVEHLDQLLQRLRGRHDQLLDDAQEVPVLASDDVASLAEEIAGIESR
                     RSGLEADTDRTIKALGSIREDLLVRERMVEEARGELQSLRHDLASLQAVQRAALGRDE
                     RSAQDWVDARGLGSAERLGEALAVVPGWEHAVESVLGDDLQGIRVDDVSPFGVALDHL
                     PGAGVTLVEGSFHAESSGELPSLSSLIRSGSLKLDSLLYGIFAAESVDVAFAHRSGLR
                     AGQSIITRQGIWVGPDWVRILPVSEQDSGIIQRTQELETLGLRVEEAELGLAELQRQV
                     VEGRGQAEELEHDRAKLQAEVNDLNVRLGDMKAQHGVKRVQIEEAGARRERIRREREE
                     LDAQVSEETRKLLEAREALAIAERTREQQDVTREELQARGAASESELAAAREEARANR
                     ERYHALNAEWQSLESRMSASETARGRLVRQRRELDEQRDNLQAGIASSATPLPELRQD
                     LEARLNERLEVERQLGAARAELEQLGER"
BASE COUNT          665 a         1102 c         1219 g          567 t
ORIGIN      
        1 gttctgcggg aaccggatcc ctcctagcgc ttcggccggc agggggtgct cgggccggtt
       61 tgggcgacgg cgaaccaatc gaaagcgggg cggtcatagc tctcatccgg ttccgtacaa
      121 cgaaggatcg ccatggcgac cagagccaac aacaatgcgg agcaacaggg ctgtgctccg
      181 gcaatcagcc tcaccgaaca gatcgcggag catctggccc gcgagatcat cacaggccga
      241 cttggcgcca atgcccggat acaggaactt cgggtggcgg acgatctggg agtgtccaga
      301 gggtcggtcc gggaagcgct gctgatactg gaaagccgcc acctcatcga tctcctgccc
      361 cgccggggcg ccatcgtcag ggatctcacg ggagaggagc tggtcagctt cagcgagctg
      421 ttcacggacc tggcggctgc ctttttcgga aagcttgccc gcgttcccga cctcgatctc
      481 ggcctgccgg cccaggcggt cgaggaaatg gcgatggccc tcggccggaa cgatctggaa
      541 ggatcgatcg cggcgaggaa ggcgttcgtc gacggctgtc ttgccgagct caacgatgtc
      601 tacctggtct ccgtgctgcg tgcgctcctg ccatcggccc tgcgtctggc ccatcgggcg
      661 gcccgacaca cgggctacga gatgcgcgat acgatccgct ttcaccatgc tctcctcgaa
      721 gcgctgtcac gcggcgacga caatcgtgtg ggggagttgg ttcgggcatt cggccggcgc
      781 gagggtcagc tggcagcgga gtgccgaagt tcctgatttt cagggtcgtt tgggtgttac
      841 gctcggtagg caattgcttt agactaccgc ggtcttaaga cagggtgatc tctcggtctc
      901 catgcggcta aagcagatca agctcgctgg cttcaagtct ttcgtcgacc cgacaaccgt
      961 catcctcccc agcaatcggt gcgccgtcgt tggccctaac ggttgcggca agtccaacat
     1021 catcgacgcg gttcgatggg tcatgggtga aagttcggcg aagcagttgc gcggtgaaaa
     1081 cctcaccgac gtcatcttca acggttccaa ttcgcgcaag cccacggcga tcgccagcat
     1141 cgagctgatc ttcagcaacg aggacggccg gatcggcggc gagtacgcgg catacgccga
     1201 gatctcggtt cggcgccagg tcacccggga agcccagtcc acctacttcc tcaacggcaa
     1261 caaatgccgg cgccgggaca tcatggacat cttcctcggc acgggattcg gtccgcgcag
     1321 ctactccatc atcgagcagg ggatgatcag ccagctcgtc gaggcgaaac ccgaagacct
     1381 gcgggtctac cttgaggaag cagccgggat ctcgaagtac aaggagcgtc gccgggagac
     1441 cgagaaccgg gttcgccata cccgggaaaa cctggaccgg ctcaacgaca tccgtgagga
     1501 actcgacagg caacttcagc atctcaagcg tcaatcccgc gcggcggagc gatatcgaga
     1561 gctcaaagag gaagagcgca agctgactgc cgagctgcac accatccgtc tggtggcgct
     1621 cgaagccgtg ctcgacgccc atcgggagcg gatccagcag ctcgaggtcg agctgagccg
     1681 ccggcaggcg gagcagcagg ccatcgacac gcagatcgag cagggtcgcc aggctcacgc
     1741 cgataccagc gccgccgtca accaggttca ggggaccttt taccagctcg gtgcggacat
     1801 cgcgcgcatc gaggaggcca ttcagttcaa ccagcagcgg gtcaagcagc tcgagctcga
     1861 cctggagacg gttacccggc gctcggagga aacgggccgt cagctggcca tggacgagac
     1921 gcagattcgc gagctgcgtg agcagctcgc cgagctggag ccgcgccggc aggaggccga
     1981 gcgcggggat ggcctggccg ccgcccgcct ggccgagctc gagcagaagc accgggcgtg
     2041 gcaggctgaa tgggacgagt tcaggaatcg agcgtctcag aacgaaaggg acgcagaggt
     2101 tcaggcctcc cgcgtggagc acctcgatca gctccttcag cgtcttcgag gccgccacga
     2161 ccagctgctt gacgatgccc aggaggtgcc cgttcttgcc agcgacgacg tggcgtccct
     2221 ggccgaagag atagccggta tcgaatcgcg gcggagcggc ctggaggccg acaccgatcg
     2281 caccatcaag gcccttggct ccatccggga agatctgctg gttcgggagc gcatggtcga
     2341 ggaggcccgt ggtgagctgc agtcgctcag acacgatctc gccagcctgc aggccgttca
     2401 gcgtgccgcc ctgggccgtg acgagcgttc tgcgcaggat tgggtcgacg cccgaggcct
     2461 cggctcggcc gagcgtctgg gtgaagctct ggcggtggtg cccggatggg aacatgccgt
     2521 tgaatcggtg ctgggcgacg atcttcaggg catacgcgtt gacgatgtga gcccgttcgg
     2581 cgttgctctg gatcacctgc cgggcgccgg cgtgaccctg gtggaaggaa gtttccacgc
     2641 cgagagcagc ggagagctgc cgagcctttc ctccctcatt agatccggga gtctcaagct
     2701 cgattccctg ctttatggga ttttcgcggc ggagtccgtc gacgtggctt ttgcgcaccg
     2761 cagcgggttg cgggccggcc agagcatcat tacccgccag gggatctggg tcggacccga
     2821 ctgggtccgt atcctgccgg tcagcgagca ggattcgggc atcatccagc ggactcagga
     2881 gctggaaacc ctcgggctgc gggtcgagga agccgagctc ggccttgccg agctgcaacg
     2941 ccaggtcgtg gaagggcgcg ggcaggccga ggagctcgaa cacgatcgag cgaagctgca
     3001 ggcagaggtc aacgacctga acgttcggct cggcgacatg aaagcccagc acggcgtgaa
     3061 aagggtgcag atcgaggagg ccggcgcccg tcgggagcgg atccgtcgcg agcgcgagga
     3121 gctcgacgct caggtttccg aggagacgcg caagcttctg gaggcaaggg aagccctggc
     3181 catcgccgag cggaccagag agcaacagga cgtcacccgt gaggagctcc aagctcgtgg
     3241 tgccgcgtcg gagtcggagc ttgccgcggc ccgggaagaa gcccgggcca atcgtgagcg
     3301 gtaccacgct ctcaacgccg agtggcagag cctggagtcc cgcatgtcgg ccagcgagac
     3361 cgcgcggggc cggctggttc gccagcgccg cgagctggac gagcagcgtg acaaccttca
     3421 ggccggcatc gcctccagcg ccacgccgct gcccgagctg cgccaggacc tggaggccag
     3481 gctgaacgag cggttggaag ttgagcggca gctcggcgcg gcgcgcgccg agctcgaaca
     3541 gcttggcgag cgg
//
LOCUS       DBNHKV010000245         3404 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1736402, whole genome shotgun sequence.
ACCESSION   DBNHKV010000245 DBNHKV010000000
VERSION     DBNHKV010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1736402"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /locus_tag="AC16PP_07850"
     CDS             complement(<1..1456)
                     /locus_tag="AC16PP_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR6481577.1"
                     /translation="MNNEPLGDTVEGETMSELTHLFQPIQIGTMNLRNRIMMPPHGGF
                     IGDLWGSEEAAEQNLAYWARRARDGVAWIDGITGFVDNTLMVPGFLPTGLGAITRGVF
                     RMPHFKERAGRYADVIHEGGACATSQLVLQGGMPHGPSARLANYTNNSVPHVLDKDEI
                     RWLVEEYAYSARQIADAGLDGLELHANHEDLLELFLSPATNHREDEYGGDLNGRLRFI
                     KEILEAIRAEVGNSITVGVRLNMDEFFEGGYDLDEGLEIAKGLDATGHVDYFHGVIGN
                     NWGAPSYIQPHHYGLANWSEMAGRFKAAVGVPVVYAGRVTTPEAGEAVLAAGHADVVG
                     MARAMFADGEIVSKSKAGRLDEIRPCIGCNDCLHAGVVEGLPFGCTVNPRTGYEHKPP
                     PAPASPPKNLLVVGGGPAGLELAAAAAERGHTVTLWERDDALGGQMRLAAQVPENRAF
                     GDFIEFQERRLARLGVTVETGREATAESIAEAGFD"
     gene            1515..2156
                     /locus_tag="AC16PP_07855"
     CDS             1515..2156
                     /locus_tag="AC16PP_07855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6481578.1"
                     /translation="MSEEPEDVQIHLDSVDHVLSTARSVRRKLDFSRPVDPEVIFDCI
                     NVSTQAPTGLGGESWRFLVVTDLGRKREIAGLYRDVLVGLQQERGLVIKPTQQALVDR
                     LHEIPAMIFVCSMGAPPGPEIPTQVAYYGSVLPSAWSLMLSLRARNLGATWTTLLSSQ
                     QEALGRILDVPEDVVFTVMLPVGYMKGARLKKADRMDARRVTFWNRWGNDAPH"
     gene            complement(2172..>3404)
                     /locus_tag="AC16PP_07860"
     CDS             complement(2172..>3404)
                     /locus_tag="AC16PP_07860"
                     /inference="COORDINATES: protein motif:HMM:NF025134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HIR6481579.1"
                     /translation="EQTQLMSKRDQRQARQQAARREKVRKQKARARSAAGKRSKPPVE
                     VTPHNLPEAMTAAVALHRDERLREAAAIYRRVLAIEPNHPDALSSLAMIEHQAARYPD
                     AIEMLTRAIAIDGANPGYHMNLAAVQDAARNQREAEACYQRAIEIEPNYPDPYYNLGD
                     LYLRQGKPDQAIDVFDACMAAIGREFHALAYKAHALDDAGRHEEARYLLNFDDYVKTY
                     AFEPSDGFESIADFNAALAAHIKTHPTLQGNVMSTEHGEHTGELLREPVGPMGAMEIR
                     IHEAVRWYIGQLPDDPQHPAVRWVPKAWKLTTWGVVMFDRGHERAHIHPNGWLSGVFY
                     LELPDLIADPARKPEGWLEFGRPTTDLHVQSELTLRHYQPAYGQMFLFPSYFYHGTVP
                     FRSDQERVCVAFDVEPLY"
BASE COUNT          500 a         1111 c         1178 g          615 t
ORIGIN      
        1 cgtcgaagcc tgcctcggcg atggattcgg cggtggcctc ccggccggtc tccacggtga
       61 cgcccagccg ggcaaggcgg cgctcctgaa attcgatgaa gtctccgaag gcccggttct
      121 cgggcacctg ggcggcaagg cgcatctgcc cgccaagggc gtcgtcgcgc tcccacaggg
      181 tgacggtatg accgcgctcg gcggcagcgg ctgccagctc caggccggcc ggccccccgc
      241 ccaccaccag caggttctta ggcgggctcg ccggcgccgg cggcggcttg tgctcgtagc
      301 ccgtgcgggg gttgaccgtg cagccgaatg gcaggccctc taccacgccc gcgtgcaggc
      361 agtcgttgca gccgatgcag ggacgaatct cgtccagacg cccggccttc gacttggaga
      421 cgatctcgcc gtcggcgaac atggcgcggg ccatgcctac cacgtcggcg tggccggcgg
      481 ccaggacggc ctccccggct tcgggcgtgg tgaccctgcc cgcgtagacc acgggcacgc
      541 ccacggcggc cttgaagcgg ccggccatct cgctccagtt ggcaaggccg tagtggtgcg
      601 gctggatgta gctgggcgcg ccccagttgt tgccgatcac cccgtggaag tagtccacgt
      661 gcccggtggc gtcgaggcct ttggcgatct cgagcccttc gtcgaggtcg tagccgcctt
      721 cgaagaactc gtccatgttc aggcgcacgc ccacggtgat ggagttgccc acctcggccc
      781 ggatggcctc caggatctcc ttgatgaagc gcagacgtcc attcagatcg ccgccgtact
      841 cgtcctcccg atgattggtg gccggggaaa gaaacagctc cagcaggtcc tcgtggttgg
      901 cgtgcagctc caggccgtcc aggccggcat ccgcaatctg ccgggccgag taggcgtact
      961 cctccaccag ccagcggatc tcgtccttgt ccagcacgtg gggcacggag ttgttggtgt
     1021 agttggcgag cctggccgac ggtccgtgag gcatgccgcc ctgcaggacg agctggctgg
     1081 tggcacaggc gccgccctcg tgaatgacgt ccgcgtagcg gccggcgcgc tccttgaagt
     1141 ggggcatgcg gaagacgccg cgggtgatgg cgccgaggcc cgtgggcaga aacccgggaa
     1201 ccatcagcgt gttgtccacg aaacccgtga tcccgtcgat ccaggcgacc ccgtcgcggg
     1261 cccgtcgggc ccagtaggcc aggttctgct cggcggcttc ctcgctgccc cagaggtcgc
     1321 cgatgaaccc gccgtgcggt ggcatcatga tgcggttgcg caggttcatg gtgccgatct
     1381 gaatcggctg aaacaggtgt gtgagctcgc tcatggtctc cccttcgacc gtatccccca
     1441 gcggctcgtt attcaccaat tgcggctcga ttacaacccc ggcgcgtggc acgatggatc
     1501 gcggaggtag cctcatgtcc gaagagcccg aagacgttca gatccatctc gacagcgtgg
     1561 atcacgtgct gagcacggcg cgctcggtgc ggcgcaagct cgacttttcc cgtccggtgg
     1621 acccggaggt gatcttcgac tgcatcaacg tctccaccca ggcgcccacc ggccttggcg
     1681 gcgaaagctg gcgcttcctg gtggtgacgg acttgggccg gaagcgggaa atcgccggcc
     1741 tttaccggga cgtgctggtg gggcttcagc aggagcgggg gctcgtcatc aagcccaccc
     1801 agcaggccct ggtggatcgg ctgcacgaga ttccggccat gatcttcgtc tgctccatgg
     1861 gcgcgccgcc ggggccggaa atacctaccc aggtggccta ctacggctcg gtgctgccct
     1921 cggcgtggtc gctgatgctg tctcttcgcg cccgcaacct gggcgccacc tggaccacgc
     1981 tgctctcctc gcagcaggaa gcgctcggcc gcatcctcga cgttcccgaa gacgtggtct
     2041 tcaccgtcat gctgccggtg ggctacatga agggggctcg cctcaagaag gccgaccgga
     2101 tggacgcccg acgggttacc ttctggaatc gctggggcaa cgacgcgccg cattgaagcg
     2161 ctgttgctgg atcagtagag cggctccacg tcgaaggcca cgcagacgcg ctcctgatcg
     2221 ctccggaacg gaacggtccc gtgatagaag taggacggga acagaaacat ctggccgtag
     2281 gccggctggt agtggcgcag ggtcagctcg gactgcacgt gcagatcggt cgtagggcgg
     2341 ccgaactcca gccagccttc gggctttcgg gctgggtcgg caatcagatc cggcagctcg
     2401 aggtagaaca cgccgctcag ccagccgttg gggtgaatgt gggcgcgctc gtggccgcgg
     2461 tcgaacatca cgacgcccca ggtggtcagc ttccaggctt tcggcaccca gcgcacggcg
     2521 ggatgctgcg gatcgtccgg cagctggccg atgtaccagc gcacggcctc gtggatccgg
     2581 atctccatgg ctcccatggg ccccaccggc tcgcgcagca gctcgcccgt gtgctcgccg
     2641 tgctcggtcg acatcacgtt cccctgcagg gtggggtggg ttttgatgtg ggctgccagc
     2701 gcggcgttga agtcagcgat gctctcgaag ccgtctgacg gctcgaatgc gtaggtcttc
     2761 acgtaatcgt cgaagttcag cagatatcgt gcttcctcgt ggcgcccggc atcgtccagg
     2821 gcgtgggctt tgtaggccag ggcgtgaaac tcgcggccga tggcggccat gcaggcgtcg
     2881 aacacgtcga tggcctggtc cggtttgccc tggcgcagat agagatcgcc gaggttgtag
     2941 taggggtccg gatagttagg ctcgatctcg atggcgcgct gatagcaggc ctccgcctcg
     3001 cgctggtttc gtgccgcgtc ctgcacggcg gccaggttca tgtggtaccc ggggttggcg
     3061 ccgtcgatgg cgatcgcgcg ggtcagcatc tcgatggcgt caggataccg cgccgcctgg
     3121 tgttcgatca tggccaggct gctcagagcg tcagggtggt tgggctcgat ggcgagcacg
     3181 cgacggtaga tggcggcggc ctcccgcaga cgctcgtcgc gatggagggc caccgccgcg
     3241 gtcatggctt cgggcaaatt gtgcggtgtg acctcgacgg gcggcttcga ccgcttgccg
     3301 gcggccgacc tcgcgcgagc cttctgctta cgcactttct cccggcgggc cgcctgctgg
     3361 cgcgcctggc gctggtcgcg tttgctcatg agttgcgttt gctc
//
LOCUS       DBNHKV010000246         2713 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1027163, whole genome shotgun sequence.
ACCESSION   DBNHKV010000246 DBNHKV010000000
VERSION     DBNHKV010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1027163"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1701
                     /gene="aspS"
                     /locus_tag="AC16PP_07865"
     CDS             <1..1701
                     /gene="aspS"
                     /locus_tag="AC16PP_07865"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR6481580.1"
                     /translation="LYGWVNRRRDHGGVIFLDVRDRTGIAQVVFDPDAPESFAVADSV
                     RNEYVLRLTGRVRPRPEGTVNAEMATGEVEVLGAELEVLNVSETPPFQLDEHSEAGED
                     VRLKYRYLDLRRPEMQSRIFARARIASAVRRFLEDEGYWEVETPTLTRATPEGARDYL
                     VPSRTHPGEFFALPQSPQVFKQLLMISGMDRYFQIARCYRDEDLRHDRQPEFTQIDIE
                     ASFVTAEDVMALTERMLKQVFSQALDVELPEFPVLTWAEAMSRYGSDKPDLRNPLELV
                     DVADLMRDVEFKVFNAPTNDPGGRVVALRAPGGGSLSRKVIDDYADFVARYGAKGLAY
                     IKVNDLGAGRAGLQSPILKFLPDEVVSEVLARVGAGDNDVIFFGADTAGVVADAMGAL
                     RDALAGDLDLLEDRWAPCWVINWPMFEMGRDGVPTPHHHPFTRPTCTPEEMLAEPMVA
                     EAAAYDVVLNGYELGGGSLRIHDREMQQAVFDGLRLGEEAEVRFGFLLDALKFGCPPH
                     GGIALGLDRLVMLMTGSHAIRDVIAFPKTQTASCPMTDAPGPVDEHQLRELHIRVRTN
                     "
     gene            1721..2461
                     /locus_tag="AC16PP_07870"
     CDS             1721..2461
                     /locus_tag="AC16PP_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6481581.1"
                     /translation="MAGHSKWANIKHRKAAQDVKRGKLYTKIIREITVAARLGGGEVA
                     DNPRLRAAMDKALGANMPKDTIERAIARGAGGQDGSDVEELVYEGYGPAGVAILVEAM
                     TDNRNRTVAEVRHAFSKHGGNLGTDGSVAYLFNRQGVISFAPGADEEQIMEVVLEAGA
                     EDIETEGDGSVSVYTPWESLSEVVGALKAEQLEPDNAEVTMVASTYSACEPEDAEKVL
                     QLIDALEELDDVQNVYSNGDFPDSVLGA"
     gene            2469..>2713
                     /locus_tag="AC16PP_07875"
     CDS             2469..>2713
                     /locus_tag="AC16PP_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR6481582.1"
                     /translation="MALRILGIDPGSRITGYGLVEVSGGRIRYLASGCVRPRAKAFPE
                     RLAEIYTGIRSVISEHGPEVLAIEQVFMARNPDSALKL"
BASE COUNT          476 a          866 c          929 g          442 t
ORIGIN      
        1 ctttacggct gggtgaatcg ccgccgtgat catggcggcg tgatctttct cgatgttcgt
       61 gaccggacgg ggattgccca ggtggtgttc gacccggatg ccccggagag cttcgcagtg
      121 gccgacagcg ttcgcaacga gtacgttctc cggctgaccg ggcgggtccg gccgcgaccc
      181 gagggcacgg tgaatgccga gatggccacc ggcgaggtgg aggtcctcgg agcggaactc
      241 gaagtgctca acgtctccga gaccccgccg tttcagctcg acgagcattc ggaagcgggc
      301 gaggacgtcc gtctcaagta ccgctacctc gacctgcgcc gcccggaaat gcagagccgc
      361 attttcgcgc gggcacgcat tgccagcgcg gtccgccgct tcctggagga cgaagggtac
      421 tgggaagtcg aaaccccgac gctgacgcgc gccacgcccg agggcgcgcg cgattatctg
      481 gttcccagcc gtacccaccc gggcgagttc ttcgcgcttc cgcagtcgcc gcaggtgttc
      541 aagcagctgc tcatgatctc gggcatggat cgctacttcc agatcgcccg ctgttaccgg
      601 gacgaggatc ttcgccacga caggcagccc gagttcacgc agatcgacat cgaggcctcg
      661 ttcgtcacgg ccgaggacgt catggcattg accgagcgca tgctcaagca ggtgttcagc
      721 caggcgctcg acgtcgagct gcccgagttt cccgtgctca cctgggccga agccatgagc
      781 cgttacggca gcgacaagcc ggacctgaga aacccgctgg aactcgtcga cgtcgccgac
      841 ctgatgcgcg acgtcgagtt caaggtcttc aacgctccga ccaacgaccc tggcggtcgt
      901 gtggtggcgc tacgggcccc cggcggaggc tcgctcagcc gcaaggtcat cgacgactat
      961 gccgatttcg tcgcgcgcta cggcgcgaag gggctcgcct acatcaaggt gaacgacctc
     1021 ggagccggca gggccggcct gcagtcgccg atcctcaagt tcctccccga cgaggtggtg
     1081 tccgaggtgc tcgcgcgcgt cggagccggt gacaacgatg tgatcttctt cggcgccgac
     1141 accgccggcg tggttgccga cgccatgggc gcgctgcgcg acgcgctggc cggcgatctc
     1201 gaccttctgg aggacagatg ggcgccgtgc tgggtgatca actggcccat gttcgagatg
     1261 gggcgcgacg gcgtcccgac gccgcaccac catcctttca cccggcctac gtgcaccccc
     1321 gaggagatgc tggccgagcc catggtcgcc gaagctgccg catacgacgt ggtgctcaac
     1381 ggctacgagc tgggcggggg ctccctccgg attcatgatc gagaaatgca gcaggccgtc
     1441 ttcgacggtc tcaggctcgg cgaggaagcg gaggtgcgct tcggttttct gctcgatgcg
     1501 ctgaagttcg ggtgtccgcc tcacggaggc atcgcgctcg ggctcgatcg tctggtgatg
     1561 ctcatgaccg gttcgcacgc gatccgcgac gtgatcgcgt ttcccaagac ccagacggcg
     1621 tcgtgtccca tgaccgatgc gcccggacct gtagacgagc atcagttgcg agagctccac
     1681 atccgcgtgc gcacgaactg aggatcaatc ggaggcgggc atggccggtc acagcaagtg
     1741 ggcgaacatc aagcacagga aagccgcgca ggatgtcaag cgcggcaagc tttacacgaa
     1801 gatcattcgt gagatcacgg tagccgcccg gctcgggggc ggcgaggtgg ccgacaaccc
     1861 gcgcctgcgc gccgccatgg acaaagccct gggcgcaaac atgccgaagg acaccataga
     1921 gcgcgccatc gcccggggcg ccggggggca ggatggcagc gacgtcgaag agctcgtcta
     1981 tgaagggtac ggcccagcgg gcgtcgcgat tctcgtcgag gccatgaccg acaaccgcaa
     2041 ccgcacggtt gcggaggtcc gacatgcgtt cagcaagcac ggagggaacc tgggcacgga
     2101 cgggtccgtg gcgtatctct tcaatcgtca gggcgtcatc agcttcgccc cgggcgctga
     2161 cgaggagcag atcatggagg tggtgctcga ggccggtgcc gaggacatag agaccgaggg
     2221 agacggctcg gtttccgtct acaccccctg ggagtcgctg agcgaggtgg tcggagcgct
     2281 caaggccgag cagctcgagc cggacaacgc cgaggtgacg atggtggcct cgacctacag
     2341 cgcgtgcgag ccggaggacg ccgagaaggt gctgcagctc atcgacgcgc tggaagagct
     2401 ggacgacgtg cagaacgtct acagcaacgg cgactttccg gacagcgtgc tgggcgcctg
     2461 aggcgaggat ggcgctccgg atccttggta tcgatccggg ttcccgcatt accggctacg
     2521 gcttggtcga ggtgtccggc ggccggatcc gctatctcgc cagcggctgc gtccggcccc
     2581 gcgcgaaagc gtttcccgag cgtctggcgg agatctacac cggcatccgg agcgtgatct
     2641 cggagcacgg ccccgaggtg ctggccatcg agcaggtatt catggcccgg aatcccgact
     2701 cggcgctgaa gct
//
LOCUS       DBNHKV010000247         5150 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_247312, whole genome shotgun sequence.
ACCESSION   DBNHKV010000247 DBNHKV010000000
VERSION     DBNHKV010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_247312"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..780
                     /locus_tag="AC16PP_07880"
     CDS             70..780
                     /locus_tag="AC16PP_07880"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6481583.1"
                     /translation="MAHDLVIRGGTIADGSGADPVPGDVAVNGTTITEVGNVEGAGRR
                     EIDARGLLVTPGFVDTHTHLDAQIGWDPMLTPVSWHGVTTALLGNCGVTFAPCHASDR
                     DFLAGMMETVEDIPKDAILGGLPWSWEDYGGYLDALQDLNPAINVAGLVGHCAVRYYV
                     MGERSIDDNSTAEERQRMAEVWPTRCGREPSASPRPGSSGTTSRTAATSRAPTPTTTS
                     SCRLRQRSASWAGSCRTC"
     gene            1137..1727
                     /locus_tag="AC16PP_07885"
     CDS             1137..1727
                     /locus_tag="AC16PP_07885"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6481584.1"
                     /translation="MFWLGDGERPDYTCDGRNSLQALADANDELPVETFLRLSRETAG
                     KALFTIRFFNQNVDALAELISTDWCLPGLGDAGAHVSQIMDSGWATFVLSHWARDAGL
                     YTVGEAVRRITSAPARIIGLSDRGTVAPGMRADLNVIDFDGLHEQMPEMVHDFPGGAP
                     RFIQRASGYRATLCNGQVILENDEHTGARSGEVIRR"
     gene            complement(1789..4218)
                     /gene="ppsA"
                     /locus_tag="AC16PP_07890"
     CDS             complement(1789..4218)
                     /gene="ppsA"
                     /locus_tag="AC16PP_07890"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017845789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HIR6481585.1"
                     /translation="MAAAPGYVRWFSEIASADLALVGGKNASLGEMYQELTSEGLRVP
                     NGFALTTRLYRDALAEAGIDGALREVLDALDVNDVESLQLGGQRCRELVYDAGLPVGS
                     REQILGAFAELRAQYGADLRVAVRSSATAEDLPHASFAGQHDSYLNISGGEALIDACR
                     RCLASVFTDRAIRYRADHGFDHLEVALSVGIMKMVRADRGSSGVIFTLDTESGFRDVI
                     LINSSWGLGENIVQGTVDPDEFYVHKPTLRQGHRCVLRRKLGEKQMRMLSGSRRGGGS
                     TYNVPTDPADRARYSLSDEDVLALADQALIIEAHYSRRAGADVPMDIEWARDAEDGEL
                     YIVQARPETVASRANPNRLVHYRLSGSAPELARGRAVGCGVATGTARVIRDPREIRDF
                     VEGDVLVADTTAPDWGAVIKKASAIVTNRGGRTCHAAIVARELGIPAVVGSGTATEQI
                     PSGDVVTVSCAEGEVGRVLAGTVAFETETMDLESLPRTRTQMMLNVGNPDVAFKMAML
                     PNDGVGLARLEFIITEYVRAHPMALLHPERIQDPGIERELRELCRGYADPAEFFVARL
                     AEGVGTIAAAFYPKPVVVRLSDFKSNEYADLLGGRAFEPVEENPMLGFRGASRYAHPA
                     YEAGFALECAALKRVRETMGLHNVRVMVPFCRRVEEAARVLATMAAHGLARGENGLEV
                     YMMCEIPNNVLLIDEFSAYFDGFSIGSNDLTQLTLGVDRDSEMVAFEFDERDPGVLRM
                     LELAVAGAKRNGKHSGICGQAPSDYPEIAEFLVRRGIDSLSLNPDSVVAATELVAQVE
                     AKIAAEGADGA"
     gene            4341..>5150
                     /locus_tag="AC16PP_07895"
     CDS             4341..>5150
                     /locus_tag="AC16PP_07895"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR6481586.1"
                     /translation="MRIGVPREIKADEYRVGMTPASVRETVEHGHEVFVQSLAGEAVG
                     LHDADYQEAGARVVQDAEAVFESAELIVKVKEPQPEEAALLRPDHILFTYLHLAPNPS
                     LTLALSESGCTAIAYETVTDTQGGLPLLTPMSAVAGRMAVQAGAHCLEIEQGGRGLLL
                     GGVPGTPPARVTVLGAGVVGSNAARVALGMEADVTILDTSLRKLAELDLHFGPALNTI
                     FSTADAVQEFVLDADLVVGAVLVPGSETPKLISEAMVTEMRKGSVLVDVAVD"
BASE COUNT          890 a         1763 c         1679 g          818 t
ORIGIN      
        1 atccttgccc gcagcaagcc ggttgatcta gcatcgatgg tttgcgtttg acgataacgg
       61 aggcaaccca tggcgcacga cctggtaata cgcggcggca ccatagccga cggcagcgga
      121 gcggacccgg tccccggtga cgtggcggtg aacggcacaa cgatcaccga ggttggcaac
      181 gtggagggcg ccggccgccg cgagatcgac gccagggggc ttctcgtgac gcccggtttc
      241 gtagacaccc acactcacct ggatgcccag atcggctggg atcccatgct cactccggta
      301 agctggcacg gcgtcaccac cgcccttctc ggcaactgcg gcgtcacctt cgctccctgc
      361 cacgcatcgg accgcgactt cctggccggg atgatggaga ccgtggaaga catccccaag
      421 gatgccatcc tcggcgggct gccctggtcg tgggaagatt acggcggtta tctggacgcc
      481 ctgcaggatc tgaatccggc catcaacgtg gccggcctcg tcggccactg cgccgtgcgc
      541 tactacgtga tgggcgagag atccatcgac gataactcca ccgccgagga gaggcagcgg
      601 atggccgaag tgtggccgac gcggtgcggg cgggagccgt cggcttctcc acgtcccggt
      661 tcctcgggca ctacatcccg gacggccgcc acgtcccggg cacccacgcc gaccacgacg
      721 agctcgtgca gattgcggca gcggtcggca agctgggcgg gctcatgcag aacgtgctga
      781 acctcggcgg tgatttcgag ggagagattt ccctgctggc cgccgaggcg cgggcgagcg
      841 ggcaacgggt gctgttcagc aacacggcgg gcaagaccca ggcctggggc gacaagctca
      901 atgcccgcat cagcgcgctg cgcgccgagg ggctggacat caacggcatc gccatacccc
      961 gcgggtccgg cttcgttacc gggcttcagg ccatgctcgt ctggcagggg cccacctggg
     1021 ggcaactcgc caagcaggac ctctcaggcc gactgcaggc catcgccgac cccgagtttc
     1081 gcgcccggct gatcgcggaa gccgaggaaa accccttgcg attcgacacg cgccagatgt
     1141 tctggctggg cgacggagaa cgtcccgact acacctgtga cggtcgcaac agcctgcagg
     1201 ccctggcgga cgccaacgac gagctgcccg tggagacgtt cctgcgcctg agccgggaga
     1261 ctgcgggcaa ggcccttttc accatccgct tcttcaatca gaacgtcgac gcgctggccg
     1321 agctgatctc aacggactgg tgcctgcccg gcctgggcga cgcgggcgcc cacgtgagcc
     1381 agatcatgga ctccggctgg gctaccttcg tgctctccca ctgggcgcgt gacgctggcc
     1441 tgtacacggt gggtgaagcc gtgcgccgga tcacttccgc gccggcgaga atcatcgggc
     1501 tgagcgaccg cggcaccgtc gcccccggca tgcgggccga tctcaacgtc atcgatttcg
     1561 acggtcttca cgagcagatg ccggagatgg tgcacgactt ccccgggggc gcgccgcggt
     1621 tcatccagcg cgccagcgga taccgcgcaa cgctctgcaa cggtcaggtg atcctggaga
     1681 acgacgagca taccggcgcg cgcagcggcg aggtgatccg ccgctgagcc gggcgggcaa
     1741 cctttacgct cgccgcccag ggcatcgccc gctccggggt cgacgcgctc aggcgccgtc
     1801 ggccccctcg gcggcaatct tcgcctccac ctgggcgacc agctcggtgg ccgctaccac
     1861 cgaatcggga ttcaggctca gcgaatcgat gccccggcgc acgaggaact cggcaatctc
     1921 gggatagtct gagggcgcct gcccgcagat gcccgagtgc ttgccattac gcttggcacc
     1981 ggcaaccgcg agttccagca tcctgagcac accgggatcc cgctcgtcga actcgaaagc
     2041 caccatctcc gagtcccggt cgacgccaag ggtaagctga gtcaggtcgt tgctgccgat
     2101 ggaaaagccg tcgaaatacg cggagaactc gtcgatcagc aggacgttgt ttgggatctc
     2161 gcacatcatg tagacctcca gaccgttttc gccccgtgcc aggccgtggg cggccatggt
     2221 ggcgagcacc cgcgctgcct cctcgacgcg ccggcagaac ggcaccatca cgcggacgtt
     2281 gtgaagaccc atggtctcgc ggacccgttt cagcgcagcg cactccagcg caaaccccgc
     2341 ctcgtaggcg ggatgggcat accgggaagc gccacggaaa cccagcatcg ggttttcctc
     2401 gacgggttcg aaggcccggc cacccagcag atcggcgtac tcgttgctct tgaagtcgga
     2461 caggcgcacc acgaccggtt tcggatagaa agcggcggcg atggttccga ccccctccgc
     2521 caggcgagcc acgaaaaact cggcggggtc ggcgtaaccg cggcacagct ctctgagctc
     2581 ccgctcgata cccggatctt ggatgcgttc cgggtgcagc agcgccatgg ggtgcgcgcg
     2641 gacgtactcg gtgatgatga actccagccg ggcaagaccg acgccgtcgt tcggcagcat
     2701 ggccatcttg aaggcgacgt cgggatttcc gacgttgagc atcatctggg ttctcgtcct
     2761 gggcagggat tccaggtcca tggtctcggt ctcgaaggcg accgtacctg ccagaacacg
     2821 tcccacctcg ccttccgcgc aggaaacggt caccacgtcg ccggatggga tctgctcggt
     2881 agccgttccc gaccccacca cggcggggat gcccagctcc cgggcaacga tggccgcatg
     2941 acacgttcgc ccgccacgat tggtgacgat ggccgaggct ttcttgatca ccgctcccca
     3001 atccggcgcg gtggtgtccg cgacgagcac gtcgccctcg acaaagtcgc gtatctcccg
     3061 tggatcccgg atcacgcggg cggtgccggt tgccaccccg cagccaacgg cccgcccgcg
     3121 cgccagctcc ggcgcagagc ccgacaaccg gtaatgcaca agccggttcg ggttcgcgcg
     3181 cgaagccacg gtttccggtc ttgcctgcac gatgtagagc tcgccgtcct cggcgtccct
     3241 ggcccactcg atgtccatgg gcacgtccgc acccgcacgg cgcgagtaat gcgcttcgat
     3301 gatcagggcc tggtcggcaa gggcgagcac gtcctcgtcg gagagcgagt accgcgcgcg
     3361 atcggccgga tccgtcggca cgttgtaggt cgatccccca ccccgccggc tccccgacaa
     3421 catgcgcatc tgcttctcgc cgagcttccg gcgcaggacg cagcggtgcc cctggcgcag
     3481 ggtgggcttg tggacataga actcgtcggg atcgacggtt ccctggacga tgttctcgcc
     3541 caggccccag gaggaattga tgagtatgac gtcgcgaaac cccgactcgg tatccagggt
     3601 gaagatcacc ccgctgctgc cccgatcggc gcggaccatc ttcatgatcc ctacggacag
     3661 ggccacctcc agatgatcga agccgtggtc tgcccggtac cggatggcgc gatcggtgaa
     3721 aacgctggcc agacagcgcc gacaggcgtc gataagcgct tcgccccccg agatgttgag
     3781 gtaggaatca tgctgaccgg cgaagctggc gtggggtagg tcctcggcgg tggcggagct
     3841 tctcaccgcc acccgcaggt ccgccccgta ctgagctctc agctcggcga acgcgccgag
     3901 aatctgctcc cggctcccca ccggcagccc ggcgtcgtag acgagctcgc ggcagcgctg
     3961 accgccgagc tgcagcgatt ccacgtcgtt gacgtccagg gcgtcgagca cctcgcgcag
     4021 agcgccgtcg atcccggcct cggcaagcgc gtcccgatac agcctcgtgg tcagggcaaa
     4081 cccgttgggc acacgaagcc cctcgctggt gagctcctga tacatctcgc cgagcgacgc
     4141 gttcttgccg cccaccaggg caagatcggc cgaggcgatt tccgagaacc atcgaacata
     4201 accgggcgct gccgccatga gccctccctc gcattgccgt ttctgctccc tcaggccccg
     4261 acgttatcta ggggtaattc cgtaatcaat ggcctttcgc ggacgtgtac aaacgcacca
     4321 cgcacaaaaa ggtgaaagac atgcgaatcg gtgtgcccag agagatcaag gccgacgagt
     4381 accgggtggg catgacgccg gcgagcgtcc gggaaaccgt cgagcacgga cacgaggtat
     4441 tcgtgcagtc cctggcaggc gaagccgttg gcctgcacga cgccgactat caggaggcgg
     4501 gcgccagggt ggtccaggac gccgaggcgg tgttcgaatc agccgagctc atcgtcaagg
     4561 tgaaggagcc ccagccggag gaagccgcgc tgctgcgccc ggaccacatc ctcttcacct
     4621 acctgcatct ggctcccaac ccttcgctca ccctggcgct gagcgaatcc ggatgcacgg
     4681 cgattgctta cgagacggtc accgacaccc agggcggctt gcccctgctg acgcccatga
     4741 gcgccgtggc cggacgcatg gccgttcagg ctggcgccca ctgtctggaa atcgagcagg
     4801 gcggccgcgg tctcctgctt ggcggcgtgc ccggcacgcc gccggccagg gtcaccgtgc
     4861 tgggcgccgg cgtcgtcggc agcaacgcgg cgcgggtggc cctgggcatg gaggccgacg
     4921 tgaccattct cgacacctcg ctgcgcaagc tcgccgagct cgacctgcat ttcggacccg
     4981 cgctcaacac catcttctcg acagcggacg ccgtgcagga gttcgtgctg gatgcggacc
     5041 tggtggtagg ggccgtgctg gtgcccggca gcgagacgcc gaagctgatc tcggaagcca
     5101 tggtgaccga gatgcgcaag ggctcggtgc tggtggacgt cgccgtcgac
//
LOCUS       DBNHKV010000248         4477 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1380255, whole genome shotgun sequence.
ACCESSION   DBNHKV010000248 DBNHKV010000000
VERSION     DBNHKV010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1380255"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="AC16PP_07900"
     CDS             <1..590
                     /locus_tag="AC16PP_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481587.1"
                     /translation="LADLFGVINIPSSIWIDEEGWIVRPAETAPAPPTPGEAASREVP
                     GEMPPRFVEMMGEAVKIQSDPVAYHAALRDWVEKGRESDYALPAEEVVARSRTRNPEI
                     ARGHAHFELATQLEIDGQHDAAIRHFREAHRLVPDSWTFRRQAWSLEPVGEGPLARFW
                     QGPDPEDPQAWPYEGDWLTDIRATGAQNYNDPWRP"
     gene            622..1800
                     /locus_tag="AC16PP_07905"
     CDS             622..1800
                     /locus_tag="AC16PP_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6481588.1"
                     /translation="MSELSEFRSDVAAWLNENCPPGARGRGQIPTGSSKVELEPDVKL
                     WLERCAERGWTAPTWPTEYGGGGHSAGETKIIYEEMAEIGARAPLMGMGLSMIGPTLL
                     EFGTPDQKERHVPSITRGEVHWCQGYSEPNAGSDLAALQTRAEDRGDHFVINGQKIWT
                     SGATLADWMFALVRTDFDAPKHEGISFVLLTMDQPGVTIKPIRLISGNSPFCETFFDD
                     AVASKADLVGELNKGWSVGKRLLQFEQSGIGGLGGANARANPGSGLAEMARRFAGGAP
                     GRIQDPDVRQAVTRFNMNRAAFRLTTQRASEENRSGTPGAATSIFKLYGAALQQDAAE
                     LRRVFLGTQGLGADEEAFSDDELETTREWLATRATTIYGGTNEVQANIIAKRVLGLPD
                     "
     gene            complement(1842..2840)
                     /locus_tag="AC16PP_07910"
     CDS             complement(1842..2840)
                     /locus_tag="AC16PP_07910"
                     /inference="COORDINATES: protein motif:HMM:NF048461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR6481589.1"
                     /translation="MPFNEFHSLPPGLLECEARELEGALGLPSLIHLPGGREPALFVS
                     VLLHGNEPSGWNGLRRLLADHPELPRSMSIFVGNVQAARAGMRALPGQQDFNRVWRGA
                     GGVEGEMARGVAGRLAERRFLAAVDLHNNTGHNPHYSVLSDLSVENLGMAYLFCDKAV
                     LIEEPDTVITHLFAGRCPAVTLELGPVSDPECEIRAYDYLLRCLRLDAVPAGHAEDFS
                     LYRSEARVHVPEGVSFHFADEGGAAPLVLTGGVEGVNFHELPAGFRFGSSEGRLEDVL
                     RVLDGAHQDVTERYFALQDGAILLKRPVVPAMYTTDPYVVHQDCLCYLMQRLEHAP"
     gene            complement(2844..4268)
                     /locus_tag="AC16PP_07915"
     CDS             complement(2844..4268)
                     /locus_tag="AC16PP_07915"
                     /inference="COORDINATES: protein motif:HMM:NF016032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HIR6481590.1"
                     /translation="MGDEIDRRYYDAEDFSDFRRRLDDETALLSDLFRRSEFSRRGDV
                     AGFELEVWLVDDEENPAPINDEYLKCLNNPLVVPELAEYNVELNGSPSALRGRVFSRL
                     HDELSATWRACRQAADRMGCALVTIGILPTVTEADLSSRYMSHMVRYQTLNDRVMALR
                     DGRPLELEIEGEGRLSTKHSDVMLEAAATSFQIHLQCRPDWAVRDFNASIVASAPIVA
                     ASANSPFLFGQSLWDETRIPLFEQAVDVGPNNPSRVSFGQGYIRESLLEIFEENRRDH
                     LILIPAVQDAPASKFSHVRFHNGTLWRWNRPLIGFDFDGQVHLRIEHRVVPAGPTIKD
                     CIANSAFYFGLIRGFGLQDEPVESRLPFEVARDNFYNCARYGLNGRVRWIRDGREEEL
                     LVRSLIMEDLLPLARRGLQSQDIPGEEIEAYLDVVAARVDSGQNGAAWQRRWVSLNGP
                     DLHGMVGAYRQQQDAGEPVHTWSL"
BASE COUNT          850 a         1465 c         1456 g          706 t
ORIGIN      
        1 ggctggccga tctgttcggc gtcatcaaca ttccgagcag catctggatc gacgaggaag
       61 gctggatcgt ccggcctgcc gagacggccc ccgcgccgcc gaccccgggg gaagcggctt
      121 cgcgagaggt acccggcgaa atgccgccac gctttgtcga gatgatgggg gaagccgtga
      181 aaattcagtc tgatccggtc gcgtaccatg ccgcgctgcg cgactgggtc gagaaaggca
      241 gggagagcga ctacgccctg cctgccgagg aggtggtcgc gcggtcacgt acccgcaatc
      301 ccgagattgc ccgcgggcac gcccacttcg agctggccac ccaactcgaa atcgacggcc
      361 agcacgacgc cgcgataagg cacttccggg aggcccaccg gctggtgccc gattcctgga
      421 cctttcgacg ccaggcctgg tctctggagc ccgtgggcga agggcctctg gcaagattct
      481 ggcaggggcc cgatcccgag gatccccagg cgtggcccta cgagggcgac tggcttaccg
      541 acatccgagc gacgggtgcc cagaactaca acgatccgtg gcgaccatga cgccccaccc
      601 tctcccactg gagcaagcaa catgtcggaa ctgagcgagt ttcggagcga cgtcgctgcc
      661 tggctgaacg aaaactgtcc acccggcgct cgcgggcgcg ggcagatccc cacgggcagc
      721 agcaaggtgg agctcgaacc ggacgtgaag ctgtggctgg agcgctgcgc cgagcgcggc
      781 tggacggcgc cgacctggcc gaccgagtac ggcggcggag gccactctgc gggcgagacg
      841 aagatcatct acgaggaaat ggccgagatc ggtgcgcggg caccgctgat gggcatgggg
      901 ctgtccatga tcgggccgac gctgctggag ttcggaaccc cggatcagaa agaaagacac
      961 gttccatcga tcacgcgcgg cgaggtgcac tggtgtcagg ggtactctga gccgaatgcc
     1021 ggctccgacc tggccgccct gcagacccgg gcagaagacc gaggggatca tttcgtgatc
     1081 aacgggcaga agatctggac ttccggagca acgctggccg actggatgtt tgccctggtg
     1141 cgcaccgact tcgatgcccc taagcacgaa ggtatcagct tcgttctcct gaccatggat
     1201 cagcccggcg tgaccataaa gcccatccgc ctgatctccg ggaactcgcc attctgcgag
     1261 accttcttcg acgacgccgt ggccagcaag gcggatctgg tcggcgaact gaacaaaggc
     1321 tggtcggtgg gcaaaaggct gctccagttc gagcaatccg gcatcggcgg ccttggcggc
     1381 gccaatgccc gggccaaccc cggcagcggc ctggcagaga tggctcgacg tttcgccggc
     1441 ggcgcgcccg gcaggatcca ggacccggat gtgcgccagg ctgtcacccg tttcaacatg
     1501 aaccgggccg cgttccggct caccacccag cgcgcctcgg aggagaacag atccggcacg
     1561 cccggcgcgg ctacatccat cttcaagctg tacggggccg cgctgcagca ggacgcggcg
     1621 gagctgcgcc gcgtgttcct cggcacccag ggtctgggag ccgacgagga agcattctca
     1681 gacgacgagc tggagacgac gcgcgaatgg ctggccacca gggcaacgac gatctacggc
     1741 gggaccaacg aggtgcaggc caacatcatc gcaaaacgcg tgctggggct cccggactga
     1801 cgagcagccc gccgtttgcc ggcaatggcg gctgaagccc gtcacggcgc gtgctctagg
     1861 cgttgcatca ggtagcaaag gcaatcctga tgcacgacgt aaggatcggt cgtgtacatg
     1921 gcagggacca ccggccgctt caacaagatg gcgccgtctt gcagagcgaa ataacgctcc
     1981 gtcacgtcct ggtgagcgcc gtccaggaca cgcagcacgt cctctaggcg gccttcggag
     2041 ctgccaaacc gaaatcccgc gggaagctcg tggaaattga ccccctccac gcctccggtc
     2101 agaaccagcg gcgcggcgcc gccttcgtca gcgaaatgaa agctcactcc ttccggcacg
     2161 tgaacgcggg cttcgctccg atagaggctg aagtcctcgg cgtgcccggc cggcaccgca
     2221 tccaggcgca ggcagcgcag cagataatcg taggcgcgaa tctcgcactc cggatcggac
     2281 accgggccga gctccagcgt gaccgccgga caccggccgg caaaaaggtg ggtaatgacg
     2341 gtatccggtt cctcgatcag cacggccttg tcgcagaaga gataggccat gcccaggttc
     2401 tccaccgaca gatcggagag aacagagtaa tgcgggttgt ggccagtgtt gttgtgcaga
     2461 tcgacggcgg ccagaaaccg gcgctcggcc aaccggccag ccacgccccg cgccatctca
     2521 ccctcaacac cacctgcccc ccgccagacg cgattgaaat cctgctgacc cggcaacgcc
     2581 cgcatccccg cccgggcggc ttgcacgttg cccacgaaaa tcgacatgga ccggggcagt
     2641 tcggggtggt cggcaagaag ccgacgcaga ccgttccagc cgctcggctc gttgccgtgc
     2701 aacagcacgg acacgaacag tgccggctcc cgcccgccgg gcagatgaat caggctgggc
     2761 aaaccgaggg ccccttccag ctcccgggct tcacactcca gcagacccgg cggcagcgag
     2821 tggaactcgt tgaaaggcac cgactagaga gaccaggtgt ggacaggttc gccggcatcc
     2881 tgctgctggc ggtaagcacc gaccatgccg tgcaggtccg ggccgttgag ggaaacccag
     2941 cgacgctgcc aggcggctcc gttctgcccc gagtcgaccc gagccgccac cacgtccaga
     3001 taggcttcga tctcctcccc cgggatgtcc tgtgactgca ggccacggcg cgccaagggg
     3061 aggaggtctt ccatgatcag gcttcgaacc agcagctcct cctcacgtcc atcccgaatc
     3121 cagcgcactc tgccgttgag cccatagcga gcgcaattgt agaagttgtc tctggcaacc
     3181 tcgaacggca gccgcgactc caccggctca tcctgcaggc caaagccgcg aatcaggccg
     3241 aaatagaatg cgctgttggc gatgcagtcc ttgatggtcg gtcctgccgg gaccacccgg
     3301 tgctcgatgc gtaggtggac ctggccgtcg aagtcgaagc cgatgagcgg gcgattccac
     3361 cgccacaggg tgccgttgtg gaaacgcacg tgactgaact tgctggccgg ggcgtcctga
     3421 accgcgggga tcaggatgag gtggtcgcgg cggttctcct cgaagatctc cagcagggac
     3481 tcgcggatgt aaccctgacc gaagctcacc cgggaggggt tgttgggtcc cacgtcgacg
     3541 gcctgctcga agagcgggat gcgggtctcg tcccaaagag actgaccgaa aagaaacggc
     3601 gagttggcgc ttgccgcgac gatgggcgca gaagccacga tgctggcgtt gaagtccctg
     3661 acggcccagt ccggccggca ctgcaggtga atctggaaag acgtggcggc cgcttcgagc
     3721 atcacgtccg agtgtttcgt ggaaagccgc ccttcaccct cgatttccag ctcgagggga
     3781 cgcccgtcgc gcagcgccat gacccgatcg ttgagtgtct gatagcggac catgtgcgac
     3841 atgtaccggg agctgaggtc ggcctcggtc accgtaggca ggatgccgat ggtcaccaga
     3901 gcgcagccca tccgatccgc ggcctgcctg caggcgcgcc aggtagcgct cagctcgtcg
     3961 tgcagccggg agaagacccg gccccggagc gcggacgggc tgccgttgag ctcgacgttg
     4021 tactcggcaa gctcaggaac gacgagcggg ttgttcaggc acttaagata ctcgtcgttg
     4081 atgggcgcgg gattttcctc gtcgtccacc agccagactt cgagctcgaa cccggccacg
     4141 tcgccccggc ggctgaactc gctgcgacga aacagatcgc tcaacagcgc ggtttcatca
     4201 tcgagtcggc ggcggaaatc gctgaagtcc tcagcatcgt agtagcgtct atcgatctcg
     4261 tcgcccatgt gaatcccgcg tctcgagctc agcctgacgg caaccgctcc gccgggccgg
     4321 ggcggcaggt ccgtctatgt cctaggaaag cgtctttttg attcgcgtgt aggcggcacc
     4381 gatctcctcg gcaagcgtct tagctgcggc gccgatttcc tcggcggact ccttcgcgcc
     4441 ttcctttacc tgagccgcac ggctctcgag gtgctgc
//
LOCUS       DBNHKV010000249        16700 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1453101, whole genome shotgun sequence.
ACCESSION   DBNHKV010000249 DBNHKV010000000
VERSION     DBNHKV010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 16700)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16700)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16700
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1453101"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="AC16PP_07920"
     CDS             <1..324
                     /locus_tag="AC16PP_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HIR6481591.1"
                     /translation="ERREELGEYYVARFETPLDSIRAYMRNLNTHRAYRRLRARRAEL
                     RAMGELPSGWELARTLTSYSERGADYVRTLHSIMKANNLDAVDDAYLRDMEPIALVPV
                     GDGAH"
     gene            complement(365..1219)
                     /locus_tag="AC16PP_07925"
     CDS             complement(365..1219)
                     /locus_tag="AC16PP_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6481592.1"
                     /translation="MSLFRIALRVIVLLALPVWAVAGTLDEIAASGEFRLAYRADTPP
                     FSSRGKGGEPEGFTIDLCRIIARDVQAALNLDEMKITWVPVTAENRFDAIVSGRAHLE
                     CGSSTVTLSRQERVDFSNLTYVTGASLMRWADSDIDRVGDLDGRRVSAIAGTTTEKVL
                     KETLERDGIDAKVVVVGDHAEALQLLLDRKIDAMAGDQATLFGIGFKTQGDDDLVITE
                     DLLSFEPYALPLRRNDADFRLAVNRSLSELYVRGDVGRSWEKWFGSHGVRPTRLLLML
                     YRLNSFSE"
     gene            complement(1305..2861)
                     /locus_tag="AC16PP_07930"
     CDS             complement(1305..2861)
                     /locus_tag="AC16PP_07930"
                     /inference="COORDINATES: protein motif:HMM:NF015746.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HIR6481593.1"
                     /translation="MREPHSSASGRGPAAMGRFLDAVERAGNRLPDPALLFFLLLLAV
                     WALSALLSQLDYGHVDPRSGDPVEVRNLLTGEALTSFFAGMVETFVGFHPLGVVLLAM
                     LGIGVADHTGFIKAGLRRLLSLTSQRLLTPMLFLIGILSHSAVDAGYVLVIPLGGVIF
                     QAAGRHPLAGIAVAFAGVSGGFSANFLPSALDPMLQGISQAGAQLMDPGLVLNPLNNW
                     FFTSASTLLVVLLGWYLTARIVEPRLADEPLDGDLADAPALDDLSDEERRGLRAGLVA
                     MLLGIALLAVTAWPDSSAWRSGGQLTAAGAPLMQSIVPLIFLLFIIPGVVYGYVSGTV
                     RGHRDVIAGMSQAMSGMGYYIVMAFFASLFIAEFGRSNLGVLVALQGAAWLEAAAMPA
                     AVTVVGVVAITGVVNLFVGSASAKWALLAPVLVPMLMALGISPDLAQAAYRVGDSSTN
                     IITPLMPYFPLVVVFAQRYVKRTGIGTIVAMMLPYSVGFLVVWTLFLLVYWMLGIPLG
                     LQASYGYPGG"
     gene            complement(2937..3377)
                     /locus_tag="AC16PP_07935"
     CDS             complement(2937..3377)
                     /locus_tag="AC16PP_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481594.1"
                     /translation="MAATVIARIAGAYLLSVLVAYLLAATAATQASLAGLEGLGVTIT
                     VGERISTTGQDLMGMLPTYGLLIAIGFAIALPIAAAFARFIPSWRAVGLVAAGAAAVG
                     LIHVGLNSVFETHLVAATRTTVGLLVQVMAGGVGGYAFYRLRDP"
     gene            3516..4547
                     /locus_tag="AC16PP_07940"
     CDS             3516..4547
                     /locus_tag="AC16PP_07940"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR6481595.1"
                     /translation="MSADYRLVEVAEGLELPWCVAFLPDGRLLVTERPGRLRQISPEG
                     EIGSPMEGIPEVLFRAQGGLFDVAPHPDFAENRLIYISYAHGTPRANTTRIARGRLDG
                     NSLKDLEVIFTARPNKTTPQHYGGKFLFLPDGTLLFTTGDGFDHREEAQDLSGLLGKT
                     VRIRDDGSIPDGNPYVRREGADPAVWTWGHRNPQGLAVDHVTGRVFLHEHGPRGGDEI
                     NLLRPGRNYGWPVITFGLDYSGARISPYQQMPGMEQPLHSWVPSIAPSGLAVYRGDVF
                     PEWQGDLFVGALVDREARRVRLRDDRVVEEEALFGELGERLRDIRVGPEGHLYLVTDE
                     GRVLRVERP"
     gene            complement(4585..5070)
                     /locus_tag="AC16PP_07945"
     CDS             complement(4585..5070)
                     /locus_tag="AC16PP_07945"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012075279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4963..4965),aa:Sec)
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIR6481596.1"
                     /translation="MSTFHDIRMESIDGEPVELSKFQNNLCLIVNVASRUGLTPQYAG
                     LRTLQEQYGDRGFTVLAFPCNQFGAQEPGSNEEILDFASSKFDVNFPMFGKIEVNGSG
                     ACELYQYLKAARADEEGNADIAWNFTKFLVDGDGNVIARFAPPVTPEEIGEQIGSLLP
                     G"
     gene            complement(5134..5577)
                     /locus_tag="AC16PP_07950"
     CDS             complement(5134..5577)
                     /locus_tag="AC16PP_07950"
                     /inference="COORDINATES: protein motif:HMM:NF015180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HIR6481597.1"
                     /translation="MIVYATMPNAPSPLPEAHPAVQPVPNGMDRFAITLSGLCLLHCL
                     LIPVVLAMAPAFSSLVLGTETTVHWVFLGMAVPTSVWALWRGYGRCQHLRALVLGIAG
                     LLVMFLGVSHLFDPRLEVPLTLVGVTMVVVAHVLNLRIPEPAPRG"
     gene            5708..6136
                     /locus_tag="AC16PP_07955"
     CDS             5708..6136
                     /locus_tag="AC16PP_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013487.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6481598.1"
                     /translation="MRGRDWLIGLVALGAASAATASEGELEYREHTMEAIGGHMQAAV
                     DILRQKVPHASHMTIHADALAALSGIVGTLFPEGSEGGDALPAIWEEPEDFAGRVEAM
                     REAATAFSAAARSNDDAALGQAFQALGQSCKGCHDNYRAD"
     gene            complement(6140..6742)
                     /locus_tag="AC16PP_07960"
     CDS             complement(6140..6742)
                     /locus_tag="AC16PP_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013460.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR6481599.1"
                     /translation="MRIWHWVLAACVTGSLYTGLAGEIALMERHQQFGYGVLGLLLFR
                     LGWGLWGGRYARFGNYRLAPRRLIEHFRGLDHGAAHTAPGMAFTIVFLLALAAQVGTG
                     LFATDDIFTQGPLSRLVENDTARALTGIHHRVYWLILAALAAHLTAHAVYALRRDPMP
                     LAMITGRKRLDLEPARAAWTAALLTALGAAALVWAMLELA"
     gene            6873..7919
                     /locus_tag="AC16PP_07965"
     CDS             6873..7919
                     /locus_tag="AC16PP_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6481600.1"
                     /translation="MALALEEHTATREGHTTFYLAAGPEDGPLIIFVHGWPELSLSWR
                     HQLPALAALGYRAVAPDMRGYGRSSTYERYEDYAQRAVVGDMMALLEGLGRTEAIWVG
                     HDWGAPTVWSIASHHPEACTAVAGLCVPYFTLERGLQACVDQVNRDVYGPEYPAGQWE
                     YQLFYQEHFDRARAEMDANPLNTVKLLFRRGDPNGAGRPAATAMTRINGGWFGPAGEA
                     PDVPRDDDVISEEDLAVYADALARNGFFGPNAYYMNHAENSAYAAEAVNDGYLDLPVL
                     FLGARYDYVCECYDSTLADPMRERCRRLTETTVYSGHWMAQERPLDVNAALVRWLATD
                     LPEVWPRSRAPDPA"
     gene            7932..9509
                     /locus_tag="AC16PP_07970"
     CDS             7932..9509
                     /locus_tag="AC16PP_07970"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR6481601.1"
                     /translation="MTTQAGGAGVPARGGLGAVACGHPATACAAAEILEEGGNAFDAV
                     IAAQFAACVAEPVLASVGGGGFLLAHPAPGAPVVYDFFVQTPRSPREASAVDFRPVHA
                     DFGTATQEFHIGLGAAATPGSLAGMEAVHGDLGSLPMARLVEPAARAAREGVRIERLQ
                     ASIFEIVKPIYAASSEARSIYGGIQEGRLLRQSDLADTLEQVARQGAGRFYRGAFADA
                     VARLAQEQGGHLSMEDLLGYGVVKRMPLSLRHAGATVLTNPPPSSGGILIGFGLQLLQ
                     AMAVADQTFGGPSHLAALAEIMAATNQARVELLAAAGSHAELDPALVERYRREVLGTP
                     RFNRGTTHISVVDAKGNAAAMTLSNGEGCGTMIPGTGVMLNNMLGEEDINPDGFGNWP
                     PDQRLTSMMAPTLVETVAGRRLALGSGGSNRIRTAIVQVISNLLDFGDSVDAAVRRPR
                     LHLEGDLLSIEDGFDRDAVRALEAGPAEIRRWPGQSLFFGGVHVAELAKDLFRAAGDP
                     RRGGVGRVVTARGAPEG"
     gene            complement(9534..11216)
                     /locus_tag="AC16PP_07975"
     CDS             complement(9534..11216)
                     /locus_tag="AC16PP_07975"
                     /inference="COORDINATES: protein motif:HMM:NF021354.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPL-N domain-containing protein"
                     /protein_id="HIR6481602.1"
                     /translation="MISSRRGGYAAFFATIVVALTLGASPADGARGGKKGGGGPVCKI
                     TDQTDVAIYVGGGIGPSSQLWAEALIDFWKTGSRIPGEPGLLNDDGGATWTGDGSLDY
                     VTLTLSEFNACRDDDFAKLELFFMPGGSAYEIQANLGASGKDRLTRFLDSGGSYVGMC
                     AGGYYAARGYYWKGDDGAPTDNCRNQFCRYEVDGTFSYEAATSDFTVHEWGGVSYHAD
                     LLAYGPLAEVLVEGPIEEIAGPWHAQSDPNHPYDSHLVTTDDPAMPWLRAVYWGGATE
                     NYVYTDNAPWGTEHAHFAADASGNDDLPFPTDGSLWTLKSVATGTGGAIMISSAHLEA
                     SLFHTASTFTDGGMTECQQYNNYTYLIRRMQSQLGAPFAVPDYDAFCAPDRAGEVKET
                     AVLFPSGLAYQNAPRIDTSGGGGGDGGGDGGGGTGSTSISFDDGTLGDFQLEGDGLRP
                     WQANATAACNGAYGAMAGVPGGVDMESFLTLTVPAGVSRVTYRYSYPSALDRGDDFHV
                     IVNGAVRKSYETGSGAQCATDSLPVTPGDELRFKCRSGGNGETCAVDEIVFD"
     gene            complement(11329..12060)
                     /locus_tag="AC16PP_07980"
     CDS             complement(11329..12060)
                     /locus_tag="AC16PP_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6481603.1"
                     /translation="MANFMLIHGAWHGGWCWHKLVAELSRRGHRALAPDLPAHGHDAT
                     PRDRVTLDAYADSVIEHLNDFAGPVCLVGHSMGGMVISAVGQRVPERLERLVYLCAFV
                     PADGMSLLSSDPTESGAQAFAGKIRGGEDGASVMIDDAAVAALFYHDCTDEDVAFARA
                     RLVPQPSAPLGAAVALTDAAFGSLAKSYIECTADQAAPIAAQRAMYTRAGIDSVVSLS
                     TSHSPFFSAPTLLADSLEALIGTPA"
     gene            complement(12071..12988)
                     /locus_tag="AC16PP_07985"
     CDS             complement(12071..12988)
                     /locus_tag="AC16PP_07985"
                     /inference="COORDINATES: protein motif:HMM:NF018649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR6481604.1"
                     /translation="MAQPTQTARGGPGRGSAFGWIWIAYLAAVVVGVAGTMIVADPAT
                     DHPVYLAGIADVLATVVIFAFSVAFRNSSFYDPYWSVVPPVLGLYWWWVAGSVDDPRV
                     WLAMLVVSCWAVRLTYNWAFGWTGLGHEDWRYRDLQNRLGILYWPVSFLGIHMMPTVL
                     VFLGCVPLFLIMGRESGPLNGIDLLALAVGAAAVWIETRADLELHRFRARRGSPAEAL
                     TTGVWGWCRHPNYLGEIGLWLSLGLFAWAAGADPVWCFAGFVAMVALFELVSIPMIEK
                     KLLAAKPGYEAYRRSTFRLLPFSALKRFS"
     gene            complement(12992..14758)
                     /locus_tag="AC16PP_07990"
     CDS             complement(12992..14758)
                     /locus_tag="AC16PP_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6481605.1"
                     /translation="MRGIVLFLGAATLLLAGCDGSAPDAPGVDRHAAAKRWITQEFQP
                     STLTPDEQAAEMAWFIEAAAPFRGMEISVVSETLTTHEYEAGVLARAFAEITGIRVSH
                     DLIQEGDVIEKLQTQMQSGRNVYDAYVNDSDLIGTHARYGYVVALSDFMAGEGADVTL
                     PTLDVDDFIGRSFTTGPDGKLYQLPDQQFANLYWFRYDWFQREDLRARFRELYGYDLG
                     VPVNWSAYEDIAEFFSEYVRTIDDQRIYGHMDYGKKDPSLGWRFTDAWLSMAGAGDPG
                     IPNGLPVDEWGIRVDGCRPVGASVSRGGAANGPAAVYALEKYIDWLKRFAPPQAPGMT
                     FSEAGPVPAQGHIAQQIFWYTTFTADMIDGDLAVVDEDGLPRWRMAPSPHGPYWEEGM
                     KLGYQDAGAWTLLASTPLERRKAAWLYAQFVVSKTVSLKKTLVGLTPIRDSDLRSAAM
                     TEAAPRLGGLVEFYRSPARTLWTPTGTNVPDYPKLAQLWWPNVANAVSGAVSAQEAMD
                     TLARDQDRVLERLQRAGIQGDCGPELNPEVDPEEWLARPGAPKPRLADEKPPGKTVPY
                     DELVADWGALPRPGEPIGRRSH"
     gene            complement(14824..15102)
                     /locus_tag="AC16PP_07995"
     CDS             complement(14824..15102)
                     /locus_tag="AC16PP_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007946969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2160 domain-containing protein"
                     /protein_id="HIR6481606.1"
                     /translation="MSFSWMAWTLPTAIFFGAILCLLVVMTVLEIRWPTRTRRGLLPL
                     ATTRGDRLFIGLLVAAFVHILWLAALDAPVLLATGISLGLLALLIRWG"
     gene            complement(15122..15922)
                     /locus_tag="AC16PP_08000"
     CDS             complement(15122..15922)
                     /locus_tag="AC16PP_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6481607.1"
                     /translation="MTRRSHWLIFIYCAYLLLPVYWLVNMALRSNRDIVGGLEIVPGR
                     PTLANFAEIVTNPVWYEGFANSLTYVGVNTVISLLVALPAAFAFSRFRFLGDRHLFFW
                     LLTNRMAPPAVFLLPFFQLYSAVGLFDTPLAVALAHCLFTVPLAVWILEGFMSAVPRS
                     LDEMARIDGHSLPYFFFRIFLPVIRGGVGVTVFFCFMFSWVELLLARTLTASAAKPIV
                     VVMTRTVSASGMDWGVLAAAGVLTIVPGVAVVWFVRRHLVKGFALGRV"
     gene            complement(15919..>16700)
                     /locus_tag="AC16PP_08005"
     CDS             complement(15919..>16700)
                     /locus_tag="AC16PP_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019470731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6481608.1"
                     /translation="MTVVNYSLQDIFDQHTRLFVGFDWYREILRDPRFLEALLRQLAF
                     SGAVLLIEVPLGVMVARSLPRSGWLAPALVVLIALPLLVPWNVVGTIWQIFARPDIGL
                     LGAALTGLGFEFNYTSSPVDAWITLLLMDVWHWTSLVALLAYAGLASIQEAHYQAAEI
                     DGAGRWQVFRLVELPRLRPVLTIAVLLRLMDSFMIYTEPFVLTGGGPGNATTFLSQYL
                     TSMAVGQFDLGPAAAFSVLYFLVTLLMCWLFYTSVIRKEDE"
BASE COUNT         3179 a         5574 c         5422 g         2525 t
ORIGIN      
        1 gaacgccgtg aggagttggg cgagtactac gtcgctcgct tcgagacacc gctggattcc
       61 atacgcgcgt acatgcggaa tctgaacacg caccgggcct accggcgcct gcgcgcacgc
      121 cgagcggaac tgcgtgccat gggtgagctt cccagcggct gggagctggc ccgcaccctg
      181 acctcatact cggaacgggg agcggactac gtccgtaccc tgcactcgat catgaaggcg
      241 aacaacctgg acgccgtgga cgatgcctac ctgcgcgaca tggagcccat agcgctggtt
      301 cccgtgggcg acggggcgca ctgagcagcc cagcgggcgg ccgcctgacg agccgtcgcg
      361 cgccctactc cgagaaggag ttgaggcggt agagcatcag cagtagccgc gtcgggcgca
      421 ccccgtggga gccgaaccac ttctcccagg accgacccac gtcacctcga acgtagagct
      481 cggagagcga gcggttgacc gccaggcgaa agtcggcgtc gttgcgcctg agcggcagag
      541 cgtatggctc aaaggagaga agatcctcgg tgatcaccag gtcgtcatca ccctgggtct
      601 tgaatccgat gccgaagagc gtggcctgat cgccagccat ggcatcgatc tttctgtcca
      661 gcagcagctg cagcgcttcc gcgtgatcac ccacgaccac caccttggca tcaatcccat
      721 ctcgctcgag ggtttccttg aggaccttct ccgtggtcgt gcccgcgatg gctgaaacgc
      781 gccgaccgtc cagatcaccg acccgatcta tgtccgagtc cgcccagcgc atgaggctgg
      841 cgccggtcac ataggtcagg ttggagaagt cgacgcgctc ctgccgggac agggtgaccg
      901 tggaggagcc gcactccaga tgcgcccggc cggagacgat ggcatcgaat cggttctccg
      961 ccgtcacagg cacccaggtg attttcatct cgtcgaggtt cagcgctgcc tggacgtcac
     1021 gggcaatgat gcggcacagg tcgatggtga agccctcggg ctcgccgccc ttgccgcgcg
     1081 acgaaaacgg cggcgtgtcc gctcggtagg caagccggaa ctcgcccgag gcggctatct
     1141 cgtccagggt tcccgccacg gcccaaaccg gcaaggccag caacacgatt acccgcaacg
     1201 cgatgcgaaa taagctcatg agaagacccc gattccaatg attctgatca gccacctact
     1261 ctagccgatc acggccggcg ggtcccctac ggcgacgatc gggctcaacc cccgggatag
     1321 ccgtagctcg cctgcaagcc cagcggaatt ccaagcatcc agtaaaccag caggaaaagc
     1381 gtccacacca cgaggaagcc cactgagtaa ggcagcatca tcgccacgat ggtgccgatg
     1441 cccgtgcgct tcacgtagcg ctgcgcgaag accaccacca ggggaaagta gggcatcagc
     1501 ggcgtgatga tgttcgtcga ggagtcgcct actcggtagg cggcctgggc gagatccggc
     1561 gagatgccca gagccatcag catcggaacc agcactggcg cgagtagcgc ccacttcgct
     1621 gaggccgagc cgacgaagag gttcaccaca ccggtgatgg ccaccacccc caccacggtg
     1681 actgccgccg gcatggcggc cgcctcgagc caggccgctc cctgcagcgc gacgagcacg
     1741 cccaggttgg accgtccgaa ctcggcgatg aacagcgaag cgaagaaggc catcacgatg
     1801 tagtaaccca tcccgctcat ggcctggctc atgcccgcga tcacgtcccg atgcccccga
     1861 accgtgccgg agacgtaacc gtagaccacg ccgggaatga tgaagagcag aaagatgagc
     1921 ggaacgatgc tctgcatgag gggcgcaccc gcggcggtca gctggccgcc cgagcgccat
     1981 gccgaactgt ccggccaggc cgtgaccgcc agcagcgcaa ttccgagcag catggcgaca
     2041 agccccgcgc gtagcccgcg acgctcttcg tcggaaagat catccagggc gggcgcatcg
     2101 gccagatcgc cgtccagggg ctcgtcagcc agccgcggct ccacgatccg agccgtcagg
     2161 taccatccga gaagcacgac gagcagcgtg gaggcggagg tgaagaacca gttgttgagc
     2221 gggttcagca ccaggcccgg atccatcagc tgcgcgccgg cctggctgat gccctgcagc
     2281 atgggatcta aggcggaggg cagaaagttc gcgctgaacc ctccggagac gccggcgaag
     2341 gccaccgcga ttcccgccag agggtgtctt ccagccgcct gaaagatgac gccgcccagg
     2401 ggtatgacga gcacgtatcc ggcatccacg gcgctgtggc tgagaatgcc gatcagaaag
     2461 agcatcgggg tgagcagcct ctgcgaagtc aggctgagca gccggcgcag ccccgccttg
     2521 atgaagccgg tgtggtcggc cacccctata ccgagcatgg cgaggagaac gacgcccagc
     2581 gggtgaaacc ccacgaacgt ctcgaccatg ccggcgaaga agctggtcag ggcctcgccg
     2641 gtcagcaggt tgcgaacctc caccggatcc ccgctgcgag ggtcgacgtg accgtagtcg
     2701 agctgcgaga gcagcgccga tagcgcccag accgccagca ggagcaagaa gaacaggagc
     2761 gccgggtcgg gtagacggtt gccggcgcgc tcgaccgcat ccaggaaccg acccatggcg
     2821 gccggcccgc gaccgctggc tgagctgtgc ggctccctca tgaatcccgc gctcccctgg
     2881 tccttctgct cttctcagga gtcgtcggct gcacgagccg actttgcctg cctcagtcac
     2941 gggtcccgca gccgatagaa agcgtagccg ccgacgccac ccgccatgac ctgaaccaga
     3001 aggcccaccg tcgtccgggt cgcggcaacg aggtgggtct cgaacaccga gttcagcccg
     3061 acatgaatga ggccgaccgc ggccgctcct gccgcgacga gcccgacggc ccgccaggaa
     3121 ggaatgaacc gggcgaaggc ggcggcaatg ggaagcgcga tggcaaaacc gatggcaatc
     3181 agcagcccgt aagtgggcaa catccccatc aggtcctggc ccgtggtgga tatccgctca
     3241 cccaccgtga tggtcacgcc cagtccttcc aggccagcca gggaggcctg ggtagcggcc
     3301 gtggcggcca ggaggtaggc gaccagcacg gataggagat aagcgcctgc gattctggcg
     3361 atcacggtcg cggccaattg tttcacctct ctgagcagat gtcctagcat agcggcttcg
     3421 cttcaccctt cgaaccctca ttcaatccgg aagtcgccat aaagccttca acgctaatct
     3481 ttctgacgct gtctctggcc atctccaccg aggcaatgtc ggccgactac cgcctggtcg
     3541 aggtcgccga gggcctggag ctgccgtggt gcgtggcctt ccttcccgac gggcggttgc
     3601 tggtcacgga gcgtcccggt cgccttcggc agatctcgcc cgagggcgag atcggctcgc
     3661 ccatggaagg aatccccgag gtgttgttcc gcgcccaggg cggcctgttc gatgtggccc
     3721 cccacccgga cttcgctgaa aaccggctga tctacatctc ctatgcccac ggcacgccgc
     3781 gagccaacac cacgcgcatc gcccgcggcc gcctggatgg caactcgctg aaggacttag
     3841 aggtgatctt tacggcccgc ccgaacaaga cgacgcccca gcactacggc ggcaagttcc
     3901 tgttccttcc cgacgggacg ttgcttttca ccaccgggga cggcttcgac caccgggagg
     3961 aagcccagga tctttccggg ctgctcggca aaaccgttcg catcagggat gacggaagca
     4021 ttcccgatgg caatccctac gtgagacgcg agggcgcgga cccggcggtg tggacctggg
     4081 ggcaccgcaa cccgcagggc ctggccgtgg atcacgtgac cggccgggta tttctccacg
     4141 agcacggtcc tcgcggcggg gacgagatca atctcctcag gcccggccgc aactacggct
     4201 ggccggtcat cacgttcggc ctcgactaca gcggcgcgcg catctctcct taccagcaga
     4261 tgcccggcat ggagcagccg ctgcactcct gggtaccttc catcgcgccg tcaggcctcg
     4321 ccgtctaccg gggcgacgtc tttcccgagt ggcagggcga cctgttcgtc ggcgcgctgg
     4381 tggacaggga agcgcggcga gtgcgcctgc gcgacgatcg cgtcgtggag gaggaggccc
     4441 tgttcgggga actgggcgag cggctgcggg acatccgggt gggcccggag ggtcacctct
     4501 acctggtcac cgacgaaggg cgggtgctgc gcgtggagcg gccctgagaa atctgggccc
     4561 cgcacggtcc ggggcccgag catgctagcc cggcagcagc gacccgatct gctcgccgat
     4621 ctcctccggc gtcacgggcg gcgcgaaacg ggcgatgacg ttcccgtccc cgtccacgag
     4681 aaacttcgtg aagttccagg cgatgtccgc attgccttcc tcgtctgcac gggcggcttt
     4741 gagatattgg tacagctcgc acgcgcccga gccgttgacc tcgatcttgc cgaacatcgg
     4801 gaagttcacg tcgaacttcg agctcgcaaa atccaggatc tcctcgttgg agcctggctc
     4861 ctgggcaccg aactggttac acgggaaggc gagcacggta aaaccccggt cgccgtattg
     4921 ttcctgaagg gtacgcagac ctgcgtactg aggggtgagg cctcagcggc tcgcgacgtt
     4981 gactatcagg cagagattgt tctgaaactt ggacagctcc acgggctcgc cgtcgatgct
     5041 ttccattcga atgtcatgaa acgtggacat gagcgttctc ctccctatgc tcgatccgaa
     5101 ccaaccgatc atagtcgatt cggcgaaacc aagtcacccg cgcggagccg gctcgggaat
     5161 tcgcaggttg agcacgtgag ccaccaccac catggtgacg ccgacaaggg tgagcggaac
     5221 ctcgagccgc ggatcgaaaa ggtgagaaac gcccaggaac atcacgagca ggccggcgat
     5281 gcccagcacc agggctcgca ggtgctggca tcttccgtag ccgcgccaga gggcccagac
     5341 gctggtcggc acggccatcc ccagaaaaac ccagtgcacg gtcgtctccg tgccgagcac
     5401 gaggctggaa aatgcaggcg ccatggccag caccaccgga atcaacaggc agtggaggag
     5461 gcagagcccg gacagcgtga tggcaaagcg gtccatgccg ttgggcacgg gctgcaccgc
     5521 tggatgcgct tccggcagag gtgacggggc attaggcatg gtggcgtaaa ctatcaccgg
     5581 gccggcccgc tgaacactgc caaagaaggg accggatctg caccgctccc ggacggcggc
     5641 aattgccgcg gagccggatt tcaaatacgg tgcgcgcaat caactccagc aacgatgagg
     5701 agagttcatg cgaggtcgag actggctgat cgggttagtg gcgctgggtg ccgccagcgc
     5761 ggccaccgca agcgaagggg agctcgaata ccgcgagcac accatggagg ccatcggcgg
     5821 gcacatgcag gcagccgtgg acatcttgag gcagaaggtg ccgcacgcct cccacatgac
     5881 catccacgcc gatgcgctgg ctgcgctgtc cggcatcgtc ggcacgctgt tccccgaagg
     5941 atccgagggc ggcgacgccc tgccggcgat ctgggaagaa cccgaggatt ttgccgggcg
     6001 ggtggaagcc atgcgcgaag cggccacggc cttcagcgct gcagcacgat ccaacgacga
     6061 tgcggcgctc ggccaggcct ttcaggccct gggtcagtcc tgcaaaggct gccacgacaa
     6121 ctaccgcgcc gactgacgct caggcgagct ccagcatcgc ccagaccaga gcagcggctc
     6181 ccaatgcggt gagcaacgcc gccgtccagg ccgcacgcgc cggctccagg tcgaggcgtt
     6241 tgcgaccggt gatcatggcc agcggcatcg ggtcacgccg cagcgcgtaa acggcgtgag
     6301 ctgtcaggtg ggcggccagg gccgcgagga tcagccagta cacccggtgg tggatgcccg
     6361 tgagcgcgcg ggccgtatcg ttctcgacga ggcggctcaa cgggccttgg gtgaagatgt
     6421 cgtcagtggc aaacaaaccc gtgccgacct gcgcggccag cgcgagcagg aacacgatgg
     6481 tgaaggccat cccgggcgcg gtgtgcgcag cgccgtggtc gaggccgcga aagtgctcaa
     6541 tcaggcgtcg aggggccagg cggtagttgc cgaaacgcgc atatcgacct ccccacaagc
     6601 cccagcccag ccgaaagagc agcaggccga gaacgccgta gccgaactgc tggtggcgtt
     6661 ccatgagagc aatctcaccc gcaagcccgg tgtagagaga gcccgttacg caggctgcca
     6721 atacccagtg ccagatcctc atgggcagtt cccagatcag ggtgcccgca gaggcgcccg
     6781 cgcccgtcgg cgaaatcctg gtaggtgccc cgtttgccat aagggatatg attcgaacaa
     6841 tcacgatgag gtcaagacac gggagaaggg ctttggcact ggcactggag gagcacaccg
     6901 caacacgcga gggccacacg acattctatc tcgccgcggg ccccgaggac ggcccgctga
     6961 tcatattcgt tcatggctgg cccgagctct ccctgagctg gcggcatcag ctacccgcgc
     7021 tggccgcgct cggttatcga gccgtcgccc ccgacatgag agggtacgga agatcctcga
     7081 cctacgagcg atacgaggac tacgctcagc gcgcggtggt gggcgacatg atggcgctgc
     7141 tggagggctt gggacgcacg gaagcgatct gggtaggtca cgactggggg gcgccgactg
     7201 tgtggagcat agcgagccat catcccgagg cctgcaccgc cgtggccggg ctctgcgtgc
     7261 cctacttcac cctggagagg ggattacagg cgtgcgtgga ccaagtgaac cgggacgtct
     7321 acgggcccga gtacccggcg gggcaatggg aataccagtt gttctatcag gagcacttcg
     7381 acagggccag ggccgaaatg gatgccaacc ccctgaacac cgtgaagctc ctgtttcgca
     7441 ggggcgaccc caacggcgcc ggccggccgg cggcgacggc gatgacccga atcaacggcg
     7501 gctggttcgg tcctgccgga gaagcgcccg acgtgcccag ggacgatgac gtgatcagcg
     7561 aagaagacct ggcggtctac gccgacgccc tcgcccgcaa cggcttcttc ggcccgaacg
     7621 cctactacat gaaccacgcc gaaaactccg cctacgcggc agaagcggtc aacgatggct
     7681 acctcgacct gccggttctg tttctcggcg cccgctacga ctacgtctgc gaatgctacg
     7741 actccacgct tgccgatccc atgcgcgagc gctgccggcg gcttaccgag accaccgtct
     7801 actccgggca ctggatggcc caggagcgtc cgctggacgt caacgcggcg ctggtacgct
     7861 ggctggcgac cgacctgccg gaggtgtggc cccgctcgcg ggcccccgac ccggcctagg
     7921 tctgcctggc tttgacgact caagccggag gggcgggcgt cccggcgcgg ggtggcctgg
     7981 gggccgtcgc ctgcggtcac ccggcaaccg cctgcgcggc cgcggagatc ctcgaggaag
     8041 gaggcaacgc cttcgacgcg gtgatcgccg cccagttcgc cgcctgcgtc gccgaaccgg
     8101 tgctggcatc cgttggcggc gggggcttcc tgctggcaca ccccgctcca ggcgcgccgg
     8161 tggtctacga cttcttcgtc cagacgccgc gctcgcctag agaggcttcg gccgtggact
     8221 ttcgtccggt ccacgccgac ttcggcaccg caacccagga attccacatc ggcctgggag
     8281 ccgcggcaac gcccggctcc ctggccggga tggaagccgt tcacggcgac ctggggagcc
     8341 tgcccatggc tcggctggtc gaaccggccg ctcgtgccgc acgggaaggc gtgcgcatag
     8401 agcgcctgca ggcaagcatc ttcgagatcg tcaagcccat ctacgcagcg agcagcgagg
     8461 cccggtcgat ctacggcggc atccaagagg ggcgcctcct gcgccagagc gacctggccg
     8521 acaccctcga gcaggtcgcg cggcaaggcg cggggcgctt ctaccgcggc gcgttcgcag
     8581 acgcggtggc acggctggcg caggagcaag gcgggcacct ttccatggag gatctgctcg
     8641 gctacggggt cgtcaaacgc atgccgttga gcctgcgcca cgccggggcc accgtgctga
     8701 ccaaccctcc gccctcctca ggcggaatcc tgatcggctt tggcctgcag cttctccagg
     8761 ccatggccgt tgccgaccaa accttcgggg gcccgtcgca tcttgccgcc ctggccgaaa
     8821 tcatggcggc aaccaatcag gcccgagtgg aattgctcgc cgcggccggc agccacgcgg
     8881 agctggaccc ggcgctcgtc gagcgttacc ggcgggaggt gctcgggacg ccgcgcttca
     8941 accgcggcac cacgcacatc agcgtcgtgg acgcaaaggg caacgccgcc gccatgaccc
     9001 tttccaacgg tgaggggtgc gggaccatga ttccaggaac cggcgtgatg ctgaacaaca
     9061 tgctgggcga ggaggacatc aacccggacg ggttcggcaa ctggccgcca gaccaacggc
     9121 tcacgtcgat gatggcgccc accctggtgg aaaccgtcgc cgggaggcgc ctggccctgg
     9181 gatcgggagg ctcgaacaga attcgcaccg ccatcgtgca ggtcatcagc aacctgctcg
     9241 acttcgggga ctccgtcgac gccgcggttc gccgaccacg gctgcatctc gagggcgatc
     9301 tgctttccat agaggacggg ttcgaccgcg atgcggttcg ggcgctggag gcaggcccgg
     9361 ccgaaattcg ccggtggccc ggccagagcc tgttcttcgg cggcgtgcat gtggccgagc
     9421 tggccaagga tctcttccgg gcagccggcg atccccgccg cggcggcgtc ggacgggttg
     9481 tgaccgcgcg cggagcgcca gagggttgac cttgctcgcc gggcgccgag gtttcagtcg
     9541 aagacgatct cgtccacggc gcaagtctcg ccgttgccgc cggaacggca tttgaaccgc
     9601 agctcatccc ccggcgtcac cggcaacgaa tccgtcgcgc actgcgcgcc gctgcccgtt
     9661 tcgtaggact ttctgaccgc gccgttgacg atgacgtgga agtcgtcgcc ccggtccaga
     9721 gcgctcgggt agctgtatcg gtaggttacc cggctcacgc ctgcaggaac ggtcagggtc
     9781 aggaacgact ccatgtccac cccgccgggc actccggcca tcgcgccgta cgcgccatta
     9841 caggcagcag tcgcattggc ctgccacggc cgcaacccgt cgccttccag ttgaaagtca
     9901 cccaaggtgc cgtcgtcgaa ggagatcgac gtcgatccgg tgccaccgcc gccatcacca
     9961 cccccatcac cgccgcctcc accagaggtg tcgattcgcg gcgcattctg gtaagccagg
    10021 ccggacggaa acaggacggc cgtctccttc acctcgccgg ccctgtccgg agcgcagaag
    10081 gcgtcatagt cgggcacggc aaatggcgcg cccagctggg actgcattcg ccggatgaga
    10141 tacgtgtagt tgttgtactg ctgacactcg gtcatgcccc cgtcggtaaa ggtgctggcc
    10201 gtgtgaaaca gggaggcctc caggtgcgcg ctggagatca tgatcgcccc gccggtgccg
    10261 gtcgccacgc tcttgagcgt ccacagcgac ccgtccgtgg gaaacggcag atcgtcattg
    10321 ccgctcgcat cggcggcaaa gtgggcgtgc tcggtgcccc acggtgcgtt gtccgtgtag
    10381 acatagttct ccgtcgcgcc tccccagtaa acggcacgaa gccagggcat ggccgggtcg
    10441 tcggtggtca ccaggtggga atcgtaggga tggttggggt cgctctgggc atgccatgga
    10501 ccggcaatct cctcgatggg cccctccacc agcacctcgg ccagcggccc gtaggcgagc
    10561 agatccgcgt gataggaaac cccgccccac tcgtgcacgg tgaagtctga tgttgccgcc
    10621 tcgtaggaaa aggtgccatc cacctcgtag cggcagaact ggttcctgca attgtccgtg
    10681 ggagcgccgt cgtcaccttt ccagtagtac ccgcgcgccg cgtagtagcc gccggcacac
    10741 atgccgacat agctgccgcc gctgtccaga aagcgcgtca gccgatcctt gccggaagcg
    10801 cccaggttcg cctgtatctc gtaggcgctc ccgcccggca tgaagaaaag ctccagcttg
    10861 gcgaagtcgt cgtccctgca ggcgttgaat tccgacagcg tgagggtgac gtagtccagg
    10921 ctgccatcgc ccgtccaggt ggccccgccg tcgtcgttga gaagcccagg ctcaccgggg
    10981 atgcggctcc cggttttcca gaagtcgatc agcgcctcgg cccacagctg gctgctgggc
    11041 cctatgccgc cgccaacgta aatcgcaacg tccgtctggt cggtgatctt gcagaccggt
    11101 ccaccgccgc ccttcttgcc gcccctcgcc ccatcggcag gcgaggcgcc caacgtgagc
    11161 gcgacgacga tcgttgcgaa aaacgcagca tacccaccac gccgcgagct gatcatgacc
    11221 cgttgcatat gtattccttc ccgtccttca cgccagtctg tgacccgtgc atgctcgacg
    11281 caacgacgac ttgttaccga ttcgtaagcc aattgcactc ccgaggcctc atgccggcgt
    11341 cccgatgagc gcctcgaggg agtcagcgag cagggtgggg gccgagaaga agggcgagtg
    11401 ggacgtgctt aggctgacca ccgagtcgat cccggcgcgg gtatacatgg ccctctgggc
    11461 agcgataggc gcggcctggt ccgccgtgca ctcgatgtag ctcttggcca ggctgccgaa
    11521 agccgcgtca gtcagggcga cggccgcacc cagcggagcg ctgggctgag gaaccaggcg
    11581 ggctctcgcg aaagccacgt cctcgtcggt gcaatcgtga tagaacaatg cggccacggc
    11641 ggcgtcatcg atcatcaccg aggcaccgtc ttcacccccg cggatctttc cggcaaaagc
    11701 ctgggctccg ctctccgtgg ggtccgagga cagcagagac atcccgtcgg caggcacgaa
    11761 cgcgcacagg tagacgagcc gctcgagccg ctcgggaacc cgctgaccga ccgccgagat
    11821 caccatgccc cccatggagt ggccgaccag gcagacgggg cctgcaaagt cattgagatg
    11881 ctcgataacg ctgtcggcat aagcgtccag cgtcacccgg tcgcggggcg tggcatcgtg
    11941 cccatgagcc ggcagatcgg gcgcgagggc ccggtgcccc cggcggctga gctcggcgac
    12001 caacttgtgc cagcaccagc ctccgtgcca ggcaccgtga atcagcatga agttcgccat
    12061 gatcggccct ctaggagaaa cgtttgagcg cggaaaaagg cagcaggcgg aacgtggacc
    12121 gccgatacgc ctcgtaaccg ggtttggcgg ccagcagctt cttctcgatc atcgggatgc
    12181 tcacgagctc gaagagcgcc accatggcga cgaagccggc gaagcaccag accggatccg
    12241 ccccggctgc ccaggcgaac agcccgagcg agagccacag gccgatctcg ccgagatagt
    12301 tgggatggcg gcaccaaccc cagaccccgg ttgtgagggc ctcggcaggt gagccgcgac
    12361 gggcccgaaa gcgatgcagc tcgagatcgg cgcgggtttc gatccagacc gccgcggccc
    12421 ccaccgccag ggccagcagg tcgatgccgt tcaggggccc cgattcccga cccatgatca
    12481 ggaaaagcgg cacgcacccg aggaagacca gcaccgtggg catcatgtgt atcccaagga
    12541 agctcacggg ccagtagagg attcccagcc tgttctgcag gtcgcgatac cgccagtctt
    12601 cgtggccaag gcccgtccag ccgaaagccc agttgtaggt caggcgtacc gcccagcagc
    12661 tgacgacgag catggcaagc cagacccgcg gatcgtctac ggagcccgca acccaccacc
    12721 agtaaaggcc cagcaccggt ggcaccacgc tccagtacgg atcgtagaaa ctggaatttc
    12781 ggaaagcgac gctgaatgcg aagatcacga cggtggccag aacgtcggca atgccggcca
    12841 gataaacggg gtgatcggtg gccgggtcgg caacgatcat cgtgccggcc acacccacca
    12901 cgacagctgc cagataggcg atccagatcc agccaaacgc gctgccccgg cccgggccgc
    12961 ccctggcagt ctgtgttggt tgtgccatgt ttcagtgact ccttcggccg atcggctcgc
    13021 ccggccgcgg caaagccccc cagtctgcca ccagctcatc gtagggaacc gtcttgccgg
    13081 gcggcttctc gtcggccagg cggggcttgg gcgcaccggg ccgtgcgagc cactcttcgg
    13141 gatccacctc cggattcagc tccggtccgc aatcgccctg gatgcccgcc cgctgaaggc
    13201 gctccagcac tctgtcctga tcccgcgcca gggtatccat ggcctcctga gcgctgacgg
    13261 cgccgctcac cgcattggcc acgttgggcc accacagctg tgccagcttc ggatagtcgg
    13321 gcacgttcgt ccccgtgggc gtccagagcg tccgcgccgg gctcctgtag aactccacca
    13381 gcccgcccag acgcggcgcg gcctcggtca tggccgccga ccgcaggtcg gaatcacgaa
    13441 tgggcgtcag ccccaccagg gttttcttga gggagacggt cttggaaacc acgaactgcg
    13501 cgtacagcca ggcagccttc ctgcgctcca gcggcgtgct ggcaagcagc gtccaagcgc
    13561 cggcatcctg ataaccgagc ttcatgcctt cctcccagta tggtccatgg ggggacggcg
    13621 ccattcgcca tcgcggcagg ccgtcttcgt cgaccacggc caggtcgccg tcgatcatgt
    13681 ccgcggtgaa ggtcgtgtac cagaaaatct gctgggcgat gtgaccctgg gccggcaccg
    13741 gcccggcttc ggagaaagtc atccccgggg cctgcggggg cgcgaagcgc ttcagccaat
    13801 cgatgtactt ctccagcgcg tagacggccg cgggcccgtt ggccgccccg ccgcgactga
    13861 cggaggcgcc gaccggacgg cagccgtcca cccggattcc ccattcgtcc acgggcaggc
    13921 cgttcgggat ccccgggtct ccggcaccgg ccatggacag ccaggcatcg gtgaagcgcc
    13981 agcccagcga cgggtccttc ttcccgtaat ccatgtggcc gtagatccgc tggtcgtcga
    14041 tggtccggac gtattcggag aagaactccg cgatgtcctc gtatgccgac cagttcacgg
    14101 gcacgccgag gtcgtagccg tagagctcgc ggaatcgggc acgaagatcc tcgcgctgga
    14161 accagtcgta tcgaaaccag tagaggttgg cgaactgctg gtccggcagc tggtagagct
    14221 tgccatccgg acccgtggta aagctccggc cgatgaaatc gtccacgtcc agggtgggaa
    14281 gggtgacgtc ggcgccctcg ccggccatga agtcggacag cgcgaccacg taaccgtaac
    14341 gcgcgtgggt gccgatgagg tcggagtcgt tgacgtaggc gtcgtagacg ttgcggccgg
    14401 actgcatctg ggtctgcagc ttctcgatga cgtcgccttc ctgaatgaga tcgtgggaca
    14461 cccggatacc cgtgatctcg gcgaaggccc gagcgagcac ccccgcttca tactcgtgag
    14521 tggtcagggt ttccgagacg acgctgattt ccatgcccct gaacggcgcg gcagcctcaa
    14581 tgaaccacgc catttccgcc gcctgctcgt ctggcgtgag cgtggagggt tgaaactcct
    14641 gggttatcca gcgcttcgcc gccgcgtgcc ggtcaacccc gggcgcgtcc ggcgcggacc
    14701 catcgcaacc ggcgagcagc aaagtcgccg ccccaagaaa aagaacgatc ccccgcatcc
    14761 gcatcccccc tggccgaacg gcgcatacct tagcaggaaa cgccaggaac cgtgctcgac
    14821 caatcagccc cagcggatga gcagcgcgag aagccccagg ctgatgccgg tcgccaagag
    14881 cacgggcgcg tcgagcgcgg ccagccagag gatgtgaacg aaggccgcca ccagcaaacc
    14941 gatgaacaga cgatcaccgc gcgtggttgc cagcggcaga agaccccggc gcgtcctcgt
    15001 cggccatcga atctcgagca ccgtcatgac cacgagcagg cagagaatcg cgccaaagaa
    15061 gatcgcggtg gggagcgtcc acgccatcca ggaaaaactc attgccctgc ggcccccgct
    15121 ttcacacccg ccccagggcg aagcccttca cgagatgcct tctgacaaac cacaccacgg
    15181 caacgcccgg gacgatggtc agcacgccag cggccgccag cacgccccag tccatgccgg
    15241 aagcgctgac cgttcgggtc atgaccacga cgatgggttt cgccgcgctc gctgtgagcg
    15301 ttcttgccag cagcagctcg acccagctga acatgaagca gaagaacacc gtcacgccaa
    15361 cgccgcctcg gatgaccggc agaaaaattc tgaagaagaa gtaggggagg ctgtggccat
    15421 cgatgcgcgc catctcgtcc aggctgcggg ggaccgccga catgaaacct tccagaatcc
    15481 acaccgccag aggcaccgtg aagaggcagt gggcgagcgc cactgccagc ggcgtgtcga
    15541 agagcccgac ggcggaatag agctgaaaga acggcagcag aaagacggca ggcggagcca
    15601 tccggttggt gagcagccag aagaaaagat gcctgtcgcc cagaaaccgg aagcgcgaga
    15661 aagcgaatgc ggctggcagc gccaccaaga gagaaatcac cgtgttcacc ccgacgtacg
    15721 taagcgagtt ggcaaaccct tcgtaccaga ccgggttggt cacgatctcc gcgaagttgg
    15781 ccagcgtagg ccggccggga acgatctcca gcccgcccac gatgtcccgg ttgctgcgca
    15841 gcgccatgtt gacaagccag taaacgggca gcagaagata cgcgcagtat atgaagatca
    15901 accagtggct gcggcgggtc attcgtcttc cttcctgatc acgctggtat agaacagcca
    15961 gcacatcagc agggtgacga ggaagtagag aaccgaaaaa gcggccgccg gccccagatc
    16021 gaactggcct accgccatgc tggtcaggta ctggctgagg aaagtggtgg cgttccccgg
    16081 gccgccgccg gtcagcacga aaggctccgt gtagatcatg aagctgtcca tcagccgcag
    16141 cagcacggca atggtcagaa cgggcctaag cctcggcagc tcgaccagcc ggaagacctg
    16201 ccagcggccg gccccatcta tctcggccgc ctgataatgg gcctcctgga tggacgcaag
    16261 accggcatag gccaggagcg ccaccaggga cgtccagtgc cagacgtcca tgagcagaag
    16321 cgtgatccag gcgtcgacgg ggctggacgt gtagttgaac tcgaaaccca ggccggtcag
    16381 cgcggcgccg agcaggccga tgtccgggcg cgcaaagatc tgccagatgg ttccgacgac
    16441 gttccaaggg accagcagcg gcaaagcgat caggacgacg agcgccggcg cgagccagcc
    16501 gctgcgcgga aggctgcgtg ccaccatgac cccgagcgga acctcgatga ggagcaccgc
    16561 gccgctgaac gcaagttgtc gcaacagcgc ctcgaggaac ctcgggtcgc gaaggatctc
    16621 tcgataccag tcgaagccga cgaacagacg cgtgtgctga tcgaagatgt cctgaaggga
    16681 gtagttgacc accgtcatga
//
LOCUS       DBNHKV010000250         2726 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1028104, whole genome shotgun sequence.
ACCESSION   DBNHKV010000250 DBNHKV010000000
VERSION     DBNHKV010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1028104"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="AC16PP_08010"
     CDS             <1..697
                     /locus_tag="AC16PP_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6481609.1"
                     /translation="IRELFAQTQEARTRGYKPGRFSFNVKGGRCEACQGDGVIKVEMH
                     FLPDIYVPCDVCKGKRYNRETLDVRYKGRNIHEVLEMTVEEAREFFDAVPMLARKLQT
                     LVDVGLSYIALGQSATTLSGGEAQRVKLSRELSKRDTGKTLYILDEPTTGLHFHDIAQ
                     LLDVLHRLRDQGNTVVVIEHNLDVIKTADWVVDLGPEGGSGGGRVVAAGTPEHLARKR
                     GSHTGRFLKKMLG"
     gene            complement(715..1161)
                     /gene="rplQ"
                     /locus_tag="AC16PP_08015"
     CDS             complement(715..1161)
                     /gene="rplQ"
                     /locus_tag="AC16PP_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR6481610.1"
                     /translation="MRHRKSGRHLNRTSSHRQAMFRNMAASLIEHEVIKTTLPKAKEL
                     RRVAEPLITLAKEDSVANRRLAFARTRSDAAVGKLFVELGPRYKDRPGGYTRILKCGN
                     RAGDAAPMAFIELVDRPVVDEFDEDEFDAADEQADEAEELEAEEKG"
     gene            complement(1252..2247)
                     /locus_tag="AC16PP_08020"
     CDS             complement(1252..2247)
                     /locus_tag="AC16PP_08020"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR6481611.1"
                     /translation="MAQSVTEFLTPRHIDIEASSPTRAKVTLEPLERGFGHTLGNTLR
                     RILLSSMPGCAITEVQIDGVLHEYSTLEGVQEDVINILLNLKGIAVILHSQDEAVITL
                     NKDGAGQVTAGDFQLTQDVEIANPDHLICSLNDNGAIRLEARVTRGRGYVPADSREEE
                     EDSRPIGVLSLDATFSPIRRVAYGVESARVEQRTDLDKLVLDLETNGTIEPEEAIRRA
                     ATILQQQLAVFVDLESEGEPVAEEKEDEIDPILIRPVDDLELTVRSANCLKAENIYYI
                     GDLIQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASLRGSDKVA"
     gene            complement(2295..>2726)
                     /gene="rpsD"
                     /locus_tag="AC16PP_08025"
     CDS             complement(2295..>2726)
                     /gene="rpsD"
                     /locus_tag="AC16PP_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR6481612.1"
                     /translation="LYGVLEKQFRNYYKKAERQKGATGENLLRLLECRLDNVVYRMGF
                     GATRAESRQLVSHKAITVNGSLVNIPSYQVAADDVVGVYEKARGQLRIQSAMQLASQR
                     APVEWVDVDPTKLEGVYRRPPDREELPPEINENLIVELYSK"
BASE COUNT          448 a          877 c          842 g          559 t
ORIGIN      
        1 gatccgtgag ctcttcgctc agacccagga ggccaggacc cggggttaca agcccggccg
       61 cttcagtttc aacgtcaagg gcgggcgctg cgaggcgtgt cagggtgacg gcgtcatcaa
      121 ggtggagatg cacttccttc ccgacatata cgtgccatgc gacgtctgca aaggcaaacg
      181 ctacaaccgg gagacgctcg acgtccgcta caaaggcagg aacatccacg aggtcctgga
      241 gatgaccgtg gaggaagccc gggaattttt cgacgcggtt cccatgcttg cacgcaagct
      301 tcagaccctg gtggacgtgg ggctgtcata catcgccctc ggacagagcg cgacgacgct
      361 ttcaggcggc gaggcgcaac gcgtcaagct gtccagggag ctttccaagc gggacaccgg
      421 caagaccctg tacatcctgg acgagcccac tacgggcctt cacttccacg acatcgccca
      481 gctgctggac gtgctgcacc gtctgcgcga tcagggaaat acggtggtcg tgatcgagca
      541 caacctggac gtcatcaaga cggccgactg ggtggtggac ctggggcccg agggcggctc
      601 tggcggcggc cgggtggtgg ccgcagggac cccggagcat ctcgccagaa agcgtggctc
      661 tcacaccggg cgttttctca agaagatgct ggggtaggtg ccttcgcgcc aggctcagcc
      721 cttctcctcg gcttccagct cttctgcttc gtccgcctgc tcgtccgccg catcgaactc
      781 gtcttcgtcg aactcgtcga ccacgggacg atccaccagc tcgatgaacg ccatgggcgc
      841 cgcatcgccc gcccgattgc cgcatttcag gatgcgggtg tagccgcccg ggcgatcttt
      901 gtagcgcggt cccaactcta cgaacagctt acccaccgcc gcgtcgctgc gcgtgcgcgc
      961 gaatgccagg cgccggttgg cgacgctgtc ttccttcgcg agggtgatga gaggctcggc
     1021 gacgcgccgg agctctttcg ctttggggag cgtcgtcttg atgacctcgt gctcaatgag
     1081 cgacgcggcc atgttacgga acatagcctg ccggtgtgag ctcgtgcgat tgagatgtcg
     1141 tccactcttg cgatgtcgca tgactgcttc ctaagttact ccgcgccttt gccgcgacga
     1201 cggcccgaga tcaccacctc cggccttcct cgacctcttc aggcgacctc ttcaggcgac
     1261 cttgtcgctg cctctgagac tggccggagg ccagttctcc aggcgcatgc caagggacag
     1321 accgcgagac gccaggacgt ccttgatctc ggtcagtgat ttcttaccga gattgggggt
     1381 cttgagcagc tcaacctcgg tgcgctgaat gaggtcgccg atgtagtaga tgttttccgc
     1441 cttgagacag ttggccgagc gcaccgtgag ctcgagatcg tccacgggtc ggatgaggat
     1501 cgggtcgatc tcgtcttcct tctcctccgc gacaggctcg ccctcgctct ccaggtccac
     1561 gaacaccgcc agctgctgct ggagaatggt cgccgcccgg cggatcgcct cctcgggctc
     1621 tatggtcccg ttggtctcca gatccagcac gagcttgtcc aggtcggttc gctgctcgac
     1681 ccgggcgctc tcgacgccgt aggcgacccg acgaatcggg ctgaaggtag cgtcgaggct
     1741 gagcacaccg atcggccggg agtcctcttc ctcttcacgg ctgtcggccg gcacgtagcc
     1801 ccgaccgcgc gtgacacggg cttccaggcg aatggcgccg ttgtcgttta gagagcagat
     1861 caagtgatcc ggattcgcga tctccacgtc ctgagtcagc tggaagtcac cggccgtcac
     1921 ctggccagcg ccgtccttgt tcagggtgat taccgcctcg tcctggctgt ggagaatcac
     1981 agcgatccct ttcaggttga gcaggatgtt gatgacgtct tcctgcacgc cttcgagggt
     2041 gctgtactcg tgcagcacgc cgtcgatctg cacctcggtg atcgcgcagc ccggcatgga
     2101 tgacagcaga atgcgccgca gcgtgttgcc cagcgtgtga ccgaagcccc gctcgagagg
     2161 ctccagcgtc accttcgccc gggtcgggct gctggcctct atgtcgatgt ggcgtggggt
     2221 cagaaattcg gttaccgact gtgccatatg tttcgtctcc tgatatctgg agtgttcagt
     2281 tactcttcgg gcccttactt ggagtagagc tccacgatga ggttctcgtt gatctccggc
     2341 ggcagctctt cccgatccgg cgggcgcctg tacacgccct cgagcttggt cgggtccacg
     2401 tccacccatt ccaccggcgc ccgctggctg gccagctgca tagccgactg gatgcgcagc
     2461 tgcccgcgtg ccttttcata cacgccgacc acgtcatcgg cggccacctg gtaggagggg
     2521 atgttgacca gcgaaccgtt caccgtgatg gccttgtgcg agaccagctg ccgtgattcc
     2581 gctctggtcg ccccgaagcc catgcgatac accacgttgt ccaggcggca ttccaaaagc
     2641 ctgagcaggt tttcgccggt ggcgcccttc tgtcgttccg ccttcttgta gtagttgcgg
     2701 aactgcttct cgaggacgcc gtaaag
//
LOCUS       DBNHKV010000251         3395 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_743594, whole genome shotgun sequence.
ACCESSION   DBNHKV010000251 DBNHKV010000000
VERSION     DBNHKV010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_743594"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /gene="lpxC"
                     /locus_tag="AC16PP_08030"
     CDS             complement(<1..898)
                     /gene="lpxC"
                     /locus_tag="AC16PP_08030"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HIR6481613.1"
                     /translation="MLRQRTIRKAVHAIGTGVHSGETVRMTLRPAGENTGVLFVRADA
                     GNATVRATVDNVCDTTLSTSIGENGTEVSTVEHLMSALWGLGIDNLVVELSGAEVPIM
                     DGSAAPFVYLINSVGVRQQFSRKQFLRVTREITVSDGDATASLTPFQGFEAEYTFVAD
                     HPVFNRYPKRAHFLHNEACYLEEVSRARSFGLVKDLEQAQTINRCLGSSLENAVGIGD
                     FEILNEDGLRYEDEFAKHKLLDAIGDLYLMGRPLLAHFRGYKSGHALNHRLAQALLHN
                     EDAWEIVTLDPDEEPSLALQPVR"
     gene            1217..2203
                     /gene="dusA"
                     /locus_tag="AC16PP_08035"
     CDS             1217..2203
                     /gene="dusA"
                     /locus_tag="AC16PP_08035"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HIR6481614.1"
                     /translation="MKTANSPAFRKLCVAPMMAWTDPHCRYLLRLCSPNARLFTEMVT
                     TGALLNGPRRELLAFDPEEHPVAIQLGGCEPADLARAAAIAQTAGFDEINLNLGCPSP
                     RVSKGRFGACLMREPELAAACIGAMRDACRLPVTAKIRLGVDDHDSDALLHDFVARLV
                     EAGCHALYLHARKAYLSGLSPAQNRDIPPLQPERAYGLKRQFAELPIILNGGISREEE
                     AAEHLRHVDGVMIGRAAYHDPMFLSRLDARLSGRAPVPLEAVVTGYLAYMDRQLLRGT
                     RLHDMTRHMLGLFKGVPGARRYRRLLSDSARLKANDLNLVHEAIAATTAQAA"
     gene            2223..2675
                     /locus_tag="AC16PP_08040"
     CDS             2223..2675
                     /locus_tag="AC16PP_08040"
                     /inference="COORDINATES: protein motif:HMM:NF016955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HIR6481615.1"
                     /translation="MLSQLLARLKEPLANSEAETAALLPLAAAVLYLEVAWADHDIAD
                     EEMQLIRRTLSEQFDLTAEEVDVIVSESKEQHDASVGLYRFTRTITEAWDEETRFQLV
                     VRLWGLALSDDELHSFEEHTIRKIAELLYVSHSRFIEAKLIAKRDAAP"
     gene            complement(2647..3162)
                     /locus_tag="AC16PP_08045"
     CDS             complement(2647..3162)
                     /locus_tag="AC16PP_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR6481616.1"
                     /translation="MAGRILVGCQDGVYVILFEGDVRLTLYTAADRFLDRMFSDVNFK
                     SVVVDLSGTESIDSTSLGLLAKLSILAKRHFDYVPTLISTRPDITRILLSMGFEDVFH
                     IVEVPLERTSQLGELPELVTTEEDMRERVIEAHRTLMSMNESNRETFQDLVAALEAEE
                     QPVMERRRASR"
     gene            complement(3164..>3395)
                     /locus_tag="AC16PP_08050"
     CDS             complement(3164..>3395)
                     /locus_tag="AC16PP_08050"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR6481617.1"
                     /translation="SYPETNVELAEGEALVIFSDGVLDALPDMDLAEKEAALLAAATR
                     AADVDEIWSSLGVQMPIDAADDMTCLLVGRER"
BASE COUNT          655 a         1074 c         1049 g          617 t
ORIGIN      
        1 ggcggacagg ctggagcgcg aggctcggct cttcatcggg gtcgagcgtg acgatctccc
       61 aggcgtcctc gttgtgcagc agcgcctgag cgagccgatg attcagagcg tgcccggatt
      121 tgtaacctcg gaagtgcgcc agtagcggcc gacccatcag gtagaggtcg ccgatggcgt
      181 cgagaagctt gtgcttggca aactcgtcct cgtatcgcag cccgtcctcg ttgaggattt
      241 cgaagtcccc gattccaacc gcgttctcga ggctcgagcc aaggcagcgg ttgatcgtct
      301 gcgcctgctc gagatctttc accagtccga aggatcgcgc gcggctcacc tcctcgaggt
      361 agcacgcttc gttgtgcaga aagtgggcgc gcttggggta tcggttgaac accggatggt
      421 cggccacaaa cgtgtactcg gcctcgaaac cctggaacgg cgtgaggctc gcggtggcat
      481 caccgtcgct gactgtgatc tcccgtgtta cccgcaggaa ctgctttcgc gagaactgct
      541 gccgaacgcc cacggaattg atgaggtaaa cgaacggtgc cgcgctgccg tccatgatcg
      601 gcacttcagc gcccgacagc tcgacgacga ggttgtctat gccgagtccc caaagggccg
      661 acatcaggtg ctcgaccgtg gacacctcgg taccgttctc gcctatcgac gtcgataggg
      721 tcgtgtcgca gacgttgtcg acggtggcac gcacggttgc gttccccgcg tcggcgcgaa
      781 cgaagaggac tcccgtattc tccccggcag ggcgtagcgt catacggact gtttcccccg
      841 aatggacacc agtaccgatc gcatgtacgg ctttgcgaat cgttctctgt ctcagcattg
      901 aagggtttct tctatcaatt tcagcaactt ggggcggaac tatacactcg atggaggaaa
      961 aaatgaagat aaaatggagg gaaatatgga gaaatatgaa gcaggtcaaa gcgcgccaat
     1021 ctggaaaaaa tgatgttttt cagaggatta cagaccgccc gatcaatcgg ctctgatggg
     1081 cttgtcagac agctgtcgcc acgctgcgcc ggcgctcgct gctcagggat atgaatgcgc
     1141 gaggcagcgc ccccagaccg tcgccaacga tccttccgct ggcattggaa aggcgcgctg
     1201 gctaagcttg cggcccatga aaacggccaa ttcgccagca ttccgcaagc tgtgcgttgc
     1261 gcccatgatg gcgtggacgg acccgcattg ccggtacctg ctgcgcctgt gcagcccgaa
     1321 cgcgcggctg ttcacggaga tggtcaccac cggcgcgctg ctgaacgggc cccggcgaga
     1381 gctgctcgcc tttgaccctg aggagcatcc ggtggccatt cagctcggcg gctgcgagcc
     1441 ggcggatctt gcccgggctg ccgcgattgc ccagaccgcc gggttcgacg agatcaacct
     1501 aaatctcggc tgcccttcac ccagggtgag caagggccgc ttcggcgcgt gcctgatgcg
     1561 ggaaccagag ctggctgccg cctgcatcgg ggccatgcgc gacgcctgca ggctgcccgt
     1621 caccgcgaag atccggctgg gggtggacga ccacgactcc gacgccctgc tccatgattt
     1681 cgttgcgcgg ctcgtcgagg ccggttgcca cgcgttgtac ctgcacgccc gcaaggctta
     1741 cttgagtggc ctttcgcccg cgcagaaccg ggacattccc ccgctgcagc cggagcgcgc
     1801 gtacggcctc aagaggcagt tcgccgagct gccgatcatt ctcaacgggg gcatatcccg
     1861 cgaggaagag gccgcggagc atcttcgaca cgtcgacgga gtcatgatcg gtcgggccgc
     1921 ttatcacgac cccatgtttc tgtcgcggct ggatgccagg ctgtcaggcc gcgctccggt
     1981 gcctctggaa gccgtggtca ccggctacct ggcctacatg gaccgacaac tccttcgagg
     2041 cacgcgtctg cacgacatga cacgccacat gctgggcctg ttcaaaggcg tccccggcgc
     2101 cagacgttac cgccggctgc tgagcgactc ggcgcggctg aaggcgaacg atctgaacct
     2161 cgtccacgaa gcgatcgcgg cgaccacggc acaggcagca tgactcccat ggagaccgcc
     2221 tgatgctcag ccagctgctc gctcggctca aggaaccgct ggcgaattcg gaagccgaaa
     2281 cggcggcgct gctacccctg gccgcagccg ttttgtacct ggaagtcgcc tgggccgatc
     2341 acgacatcgc cgatgaggaa atgcagctga tccgccgaac gctctcggag cagttcgacc
     2401 tcacggccga agaggtggac gtgatcgtga gcgaatccaa agagcagcac gacgccagcg
     2461 tggggctgta ccgattcacc cgcaccatca ccgaggcgtg ggacgaggaa accaggtttc
     2521 agctcgtcgt ccggctctgg ggtctggcac tgtcagacga cgagctgcac agcttcgaag
     2581 agcacactat ccgcaagatc gccgagctgc tgtatgtcag ccatagccgc tttatcgagg
     2641 ccaagctcat cgcgaagcgc gacgccgctc cataacgggc tgctcttcgg cttcgagagc
     2701 ggcgacaagg tcctgaaacg tctcgcgatt ggattcgttc atcgacatca gtgtccggtg
     2761 agcttcgatg actcgctcac gcatgtcttc ttcagtggtc accaactcgg ggagctcgcc
     2821 caactgggac gtgcgctcca gagggacctc cacgatatgg aagacatctt cgaatcccat
     2881 cgagagcagg atccgcgtga tgtccggacg ggtcgaaatc aatgtcggga cgtagtcgaa
     2941 gtgccgcttg gccaggatag agagcttggc gagcaaaccg agcgaggtgc tgtcgatgct
     3001 ctcggtaccc gacagatcca ccaccacgga tttgaaattg acgtcgctga acatccggtc
     3061 gaggaaccgg tcggcggccg tgtacaacgt cagccggacg tctccctcga agaggatgac
     3121 gtacacacca tcctggcagc caaccagaat gcgacccgcc acctcagcgc tcccgcccga
     3181 cgagcagaca ggtcatgtca tcagccgcat ctatcggcat ctgtactccc agcgaagacc
     3241 agatctcgtc gacgtcggca gcgcgggtcg ccgcggcgag cagcgcggcc tccttctcgg
     3301 ccaggtccat gtccggcagg gcgtcgagca ccccgtccga gaagatcacc agagcctcgc
     3361 cctcggccag ctcgacgttc gtctcggggt agctg
//
LOCUS       DBNHKV010000252         4297 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_601839, whole genome shotgun sequence.
ACCESSION   DBNHKV010000252 DBNHKV010000000
VERSION     DBNHKV010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_601839"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="AC16PP_08055"
     CDS             <1..191
                     /locus_tag="AC16PP_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR6481618.1"
                     /translation="AEETKQGEHYVRVERRHGKFARQFRVPPSARPEDLRANVKDGVL
                     TIHVPKGGAPETRRVPVS"
     gene            201..677
                     /locus_tag="AC16PP_08060"
     CDS             201..677
                     /locus_tag="AC16PP_08060"
                     /inference="COORDINATES: protein motif:HMM:NF037143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR6481619.1"
                     /translation="MDLNQIKADVGQTWKWLADGWRSLSDKAVNALTYFTPGRNEPAD
                     PDEIRWGLLAADVSESGEHVTVELEAPGLEKDEIDVSVDERQVTITGQKRVESERREG
                     AMVVRERAFGRFQRTIPMPVAVTADGAEASYRRGVLRIRVPKVASPGGRSIKIEKG"
     gene            complement(658..1182)
                     /locus_tag="AC16PP_08065"
     CDS             complement(658..1182)
                     /locus_tag="AC16PP_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIR6481620.1"
                     /translation="MVENIIQLIEEVAEALLENRSRLITAESCTGGMVAAELTSRPGS
                     SEWFEGALVTYRLSAKQRLLGVPAAMLDRYGPVSEPVARAMAEGALRVSDADVSVAIT
                     GIAGPDGGDMLAPVGTVWFAWARRDEPGAEPVCLQATQHRFSGSRDEVRTQAVALALE
                     GVLASLSGINPSRS"
     gene            1254..3692
                     /locus_tag="AC16PP_08070"
     CDS             1254..3692
                     /locus_tag="AC16PP_08070"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6481621.1"
                     /translation="MTTYLRALNYPRTLLTLVALLLVVAGVCGTRFSFDASSDTLVVE
                     GDPDLATYLRIAETFGGDEFLLLTFTPRDGDALDPANLATLRALQQEIAALEGVGSVF
                     SILDAPLLKSPPVPLAELARGFRTLESADVDRPSAREELKESPVFGGLLITADARTTA
                     MRVDISLDRELLVLDEERARLRSQAERSAAEVKRLAELEVARETLREAYVNGRERLIG
                     QIRDVRDRYADRGVLHLGGVPMIAADMIAFVKSDLVVFGGSVVVIVMVTLYGFFRRLR
                     WVLLPVVTSAVTIGFTVGVLGLAQWPATVISSNFVSLLAIITISLTIHLVVRYRELAH
                     LHPDMAADELVRQTMVSKFAPCLYNALTTIAAFGSLTASQIVPVEDFGWMMCIGIAIG
                     FFTSFTLFPALLLLLGRVAGPSLLSRELGVTRVFSHLARWRAPLVVVISAVWAAFAAL
                     GVSMVSMDNRFLEYFRENTDINQGMAFVDAQLGGTVPFDVVLEFPPFENLVEEEDDFF
                     SEEETYPERYWFTRDKLDKLMLVHRYVEARPEVGKVLSLTSLEDLARQFTEGDALSNL
                     EVAAVLDVLPEHLRDELIRPYASPSTGELRINARVIESGRSFDRQALAADIRDFAVTQ
                     GAVPADAVQVTGMMVLFDSMLKQLLSSQLDTLVYVLLATFAMFVILLRSFLYALLGLI
                     PNVIAAASVIALMGYVGIPLDMMTITIAAISIGIGVDDAIHYLHRFDEERRRREDVRM
                     AVAWSHATIGRAMYFTSVTVIIGFSVLVFSNFVPTIFFGLLTAAAMALALIANLTLLP
                     SLLVLVIGRREASA"
     gene            complement(3784..>4297)
                     /locus_tag="AC16PP_08075"
     CDS             complement(3784..>4297)
                     /locus_tag="AC16PP_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003194700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HIR6481622.1"
                     /translation="LRADPKEIAEHLMLIDLGRNDVGRVAQIGSVEVTEKFVIERYSH
                     VMHISSNVVGRLAEGKTAIDVLRATLPVGTLSGAPKIRAMEIIDELEPVKRGIYGGAV
                     GYLAWNGNMDTAIAIRTAVIKDGTLYIEAGGGVVADSVPRLEWKESMNKGRAIFRAAA
                     MAENRFRDLG"
BASE COUNT          683 a         1418 c         1393 g          803 t
ORIGIN      
        1 cggccgagga gaccaagcag ggcgagcact acgttcgcgt agagcgccgt catggtaagt
       61 tcgcccgcca gttccgcgtt ccgccgagcg cgaggccgga ggatctgcgc gcgaacgtca
      121 aggacggtgt gctcaccatt cacgttccca aaggcggcgc accggagacc cggcgcgtgc
      181 ccgtcagcta ggagcccgcc atggacctca atcagatcaa ggccgatgtg gggcagacct
      241 ggaagtggct ggcagacggt tggcgcagcc tgagcgacaa ggccgtcaac gcgctcacat
      301 actttacgcc ggggcgcaac gagccggccg accccgatga gatccgctgg gggctcctgg
      361 ccgccgatgt gtcggaaagc ggcgagcacg tgaccgtcga gctcgaagcg ccggggcttg
      421 agaaggacga gatcgacgtg tccgtggacg agcggcaggt gacgatcacg ggtcagaaga
      481 gagtcgaatc cgagcggcgt gaaggagcca tggtggttcg ggagcgcgcg ttcggccgat
      541 ttcagcggac gatccccatg ccagtggcag tcaccgccga tggcgccgag gcgagctacc
      601 ggcgcggtgt gctcaggatc cgggtgccca aggtggcaag cccgggcggc cgaagcatca
      661 agatcgagaa gggttgatcc cgctcaggct cgcgagcacg ccctccaggg ccagggcgac
      721 ggcctgagtt cgaacctcgt ctcgagatcc cgagaagcga tgctgagtcg cctgcaggca
      781 gacgggctcg gcgccgggct cgtcgcgcct ggcccaggca aaccaaaccg tgcccactgg
      841 ggccagcatg tcgccaccgt cgggaccggc aatgcccgtt atcgcgacgc tcacgtccgc
      901 atcgctgacg cgcagcgcgc cctctgccat cgcccgggcg acgggctcgc tgaccgggcc
      961 gtagcggtcg agcattgccg ccggcacgcc gagcaggcgc tgcttggcgc tcagacgata
     1021 cgtcaccagc gccccctcga accactccga gctgccgggt cggctggtga gctccgctgc
     1081 caccatgccc ccggtgcagg actcggcggt gatcaggcgc gaacggttct cgagaagggc
     1141 ctcggccact tcctctatga gttgaataat gttttccacc accgaacaat agccgaaaat
     1201 tccttgccgc gagcacgcgt atctccgatc tgcacaaaca cctgcaaccg ctggtgacga
     1261 cctacctgcg ggcgctgaac tatccgcgca cgctgctcac cctggtggcg ctgctgctgg
     1321 ttgtcgcggg cgtttgcggg acgcgattca gcttcgatgc ctcctcggac acgctggtgg
     1381 tggagggcga tccggacctc gccacctacc tgagaatcgc cgagactttc ggcggcgacg
     1441 agtttcttct gctcacgttc acgcccaggg acggtgacgc gctggatccg gccaatctcg
     1501 ccaccctgcg cgccttgcag caggagatag ccgctctgga gggcgtcggc tcggtcttct
     1561 ccattctcga tgcgccgttg ctgaagagtc ctcccgtgcc gctggccgag ctggcccggg
     1621 gttttcgcac gctggagtcg gcggacgtgg accggccctc ggccagggag gagctcaagg
     1681 agagcccggt gttcggcggg ctcctcatca cggcagatgc tcgcaccacg gccatgcggg
     1741 tggacatctc gctggatcgg gagcttctcg tgctcgacga ggaacgggcc cggctgcgaa
     1801 gccaggccga gaggtccgcg gcggaggtca agcggctggc ggagctcgag gttgcccggg
     1861 agacgttgag ggaagcctac gtcaacggcc gggaacgtct gatcggccag atacgcgatg
     1921 tgcgcgaccg ctacgcggat cgaggcgttc tccacctggg cggcgtgccg atgatcgcgg
     1981 cagacatgat cgccttcgtg aagagcgacc tcgtggtttt tggcggcagc gtcgtggtga
     2041 tcgtgatggt cacgctctac gggtttttcc gacggctgcg ctgggtcctg cttccggtcg
     2101 taaccagcgc cgtcaccatc ggcttcaccg tgggcgtcct cggactggcc caatggccgg
     2161 ctaccgtcat ctcttccaac ttcgtctccc tgctggcgat catcaccatc tcgctgacca
     2221 ttcatctcgt ggtccgctat cgcgagcttg cccacctgca tcccgacatg gcggcggacg
     2281 agctggtccg gcaaaccatg gtcagcaagt ttgcgccgtg cctgtacaac gcgttgacca
     2341 ccattgccgc cttcggatcg ctgaccgcca gccagatcgt cccggtggag gatttcggct
     2401 ggatgatgtg catcggcatc gccatcggtt tcttcacgag cttcaccctc ttcccggcgc
     2461 tgctgcttct gctcggccgg gtcgcggggc cgtccctgct gagtcgggag ctgggggtta
     2521 cccgtgtctt cagccacctg gcccgctggc gcgcaccgct ggtggtggtc atctccgccg
     2581 tgtgggcggc gtttgccgcc ctcggggtga gcatggtttc catggacaac cgcttcctcg
     2641 agtactttcg agagaacacg gacatcaacc aggggatggc cttcgtcgat gcccagctgg
     2701 gcggcacggt gcccttcgac gtggtcctcg agttcccgcc gttcgaaaac ctggtcgagg
     2761 aagaggacga cttcttctcg gaggaagaga cctatcccga gcggtactgg ttcacccggg
     2821 acaagctgga caagctgatg ctcgtccacc ggtacgtcga ggcccgcccg gaggtgggaa
     2881 aggttctctc gctgacgtcg ctggaggatc tggccaggca gttcaccgag ggcgatgcgc
     2941 tttccaacct cgaggtcgcc gcggttctgg acgtgctgcc cgagcacttg agggacgagc
     3001 tcatccggcc ctacgcgtct ccgtccacgg gcgagttgcg gatcaacgcg cgcgtcatcg
     3061 agagcgggcg gtcgttcgac cgtcaggccc tggccgccga catccgcgat ttcgccgtga
     3121 cccagggcgc ggtgccagcc gatgccgttc aggtgacggg catgatggtt ctattcgaca
     3181 gcatgctcaa gcagctgctc agctctcagc tggatacgct cgtctacgtg ctcctggcca
     3241 ccttcgccat gttcgtcatc cttctgcgct cctttctcta cgcgttgctc ggactgattc
     3301 ccaacgtgat tgcggcggcc agcgtcatcg cgctcatggg ttacgtgggc atacccctgg
     3361 acatgatgac catcaccatc gccgccatca gcatcgggat cggcgtggac gacgccatcc
     3421 actatctgca ccgcttcgac gaggagcgcc gcaggcggga agacgttcgc atggcggtgg
     3481 cgtggagtca cgccaccatt ggccgcgcca tgtacttcac cagcgtgacg gtgatcatcg
     3541 gcttctccgt tctcgtgttc tcgaacttcg ttccgaccat cttcttcggg ttgctgaccg
     3601 cggccgccat ggccctggct ctgatcgcga acctgacgct tctgccgagc ctgctcgtgc
     3661 tggtgatcgg gcgacgcgaa gcctccgcgt gacgcttcac tcgccaacgg tgggtcgagt
     3721 ggcgtttgct tccttgagat ggttcggctt cgcggtaacg aacggattca cgacgacgtc
     3781 tttctagccc agatcccgga agcgattctc ggccatggcg gccgcccgga agatggcacg
     3841 ccccttgttc atgctttcct tccattccag gcgtggcacc gagtcggcca ccacgccgcc
     3901 gcccgcttcg atgtacagcg tgccgtcctt gatcaccgcg gtgcgaatcg cgatcgccgt
     3961 atccatgttg ccgttccatg ccaggtaacc aacggcgccg ccgtagatgc cccgcttcac
     4021 gggctccagc tcgtcgatga tctccatggc ccggatcttc ggggcgccgg acagcgtgcc
     4081 caccggcagc gtcgcgcgca gcacgtcgat ggccgtcttg ccttccgcca gccgtcccac
     4141 cacgttgctg gaaatgtgca tgacgtggga atagcgctcg atgacgaact tctcggtgac
     4201 ctccacggag ccgatctgcg ccacccggcc cacgtcgttg cggccgagat cgatgagcat
     4261 caggtgctcg gcgatctctt tgggatcggc ccggagc
//
LOCUS       DBNHKV010000253         5597 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_532185, whole genome shotgun sequence.
ACCESSION   DBNHKV010000253 DBNHKV010000000
VERSION     DBNHKV010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_532185"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /gene="miaB"
                     /locus_tag="AC16PP_08080"
     CDS             <1..870
                     /gene="miaB"
                     /locus_tag="AC16PP_08080"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR6481623.1"
                     /translation="MEGCSKYCSFCVVPYTRGEEVSRPVADVMREVIQLAGQGVREVN
                     LLGQNVNSYLGPIDDDHADLAELIYYVAEVDGIDRIRFTTSHPLDFNDSLVRAFADVP
                     ELVDHLHLPVQSGSDRVLALMKRGHTVSEYREKIAALKEVRGGISLSSDFIVGFPGET
                     EADFQATMDLVAEIGFDTSFSFVYSARPGTPAAALPDDTPEAVKRERLALLQGLLRSQ
                     ADAIACDMVGTRQTVLVTGESRKNREELQARTENNRVVNFPCPDRRLVGGFVDVDITE
                     ALPNSLRGVMAIT"
     gene            963..2000
                     /locus_tag="AC16PP_08085"
     CDS             963..2000
                     /locus_tag="AC16PP_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR6481624.1"
                     /translation="MSEDDSRVVALTTNVSLEPNEPRRLAALCGPFDQNLKHLERRLG
                     VHIRSRGNLFQISGPEQRVQAAGALLSQLYRETLEHETIDPDMVHLFLQESGVDELLA
                     RQRGPDEMPADGEGAVISTGRKSVKPRGGNQTRYVERIRGHDVNFGIGPAGTGKTYLA
                     VACAVQALESQQVSRILLVRPAVEAGEKLGFLPGDLAQKIDPYLRPLYDALYEMMGVD
                     RVNKAIERNIIEVAPLAYMRGRTLNNAFIILDESQNTTIAQMKMFLTRIGFGSTAVIT
                     GDITQIDLPKGQKSGLVNVRNVLSGIDGISFTHFGSKDVVRHPLVQKIVDAYEAYQEP
                     HASPDSDRDAG"
     gene            1990..2445
                     /gene="ybeY"
                     /locus_tag="AC16PP_08090"
     CDS             1990..2445
                     /gene="ybeY"
                     /locus_tag="AC16PP_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017903146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR6481625.1"
                     /translation="MPVEVQIASVQAGLPSVARIEGWASAALDELGDEGEPPELCIRV
                     VDGEESRELNARFRGHDKPTNVLSFPADVDLPEAKLLGDVVICAPVVGVEAAEQGKSP
                     EDHFAHMVVHGVLHLSGYDHEQAGEAELMEAIERRILERVGIADPYAER"
     gene            2452..3287
                     /locus_tag="AC16PP_08095"
                     /pseudo
     CDS             2452..3287
                     /locus_tag="AC16PP_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798742.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
     gene            3284..4765
                     /gene="lnt"
                     /locus_tag="AC16PP_08100"
     CDS             3284..4765
                     /gene="lnt"
                     /locus_tag="AC16PP_08100"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR6481626.1"
                     /translation="MKPLLALACGALVPLSLAPFGWWPLGLVAVGGWFALLQSSDRGG
                     LLLGWLFGLGKYAAGVSWIYVSINVYGNAPPPLAAFLVALFAGGLALFPMVNAWVFQR
                     LRTKGAPLVNAWLFACLFVAFEWLLTWFLTGFPWLYPAYGHVETVLGGLAPAGGVMLV
                     SLGLAGSACSVAAAHQCARRLPVLTAALAPWVVGAVLGAVSWVEPGAVRTVALVQGNV
                     DQAVKWLPENRRPIIDRYRGLSEDHWGVDVMVWPEAAITVMAHDAGEVLGDLEREGER
                     SGTAVVLGLPAVERLADDRIVLRNTAIALGDGSGRYVKRRLVPFGEYVPLEGWLRGLI
                     EFFDLPMSRAAPGDWRQPLLRVGQEQAALAICYEIVYPDLVRAQAVGADVLVTISNDT
                     WFGASIGPLQHLQMARMRALENGRWLLRGTNNGVTAIVDHRGRLVARLPQFEAGVLRG
                     EYRLVAGRTPYNRHGDGPVLVLVAFGLGVAFWRRLGAARGADG"
     gene            4781..5311
                     /locus_tag="AC16PP_08105"
     CDS             4781..5311
                     /locus_tag="AC16PP_08105"
                     /inference="COORDINATES: protein motif:HMM:NF016290.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly lipoprotein LptE"
                     /protein_id="HIR6481627.1"
                     /translation="MRTRLRYTWLIPPLFLLIGGCGFQLRTWDLGTSVESFYVRDASN
                     NPLASPLRRALRQAGVDEAGNASDAQVVVLLLNAQRGRRSVSVTGQARAAEYEISLGV
                     QYGVLDGQGQELIAPRWVRGTRVFRVDRDNIVGTSEEQALLEREIQNDLIQQIVRALN
                     TVSGPAGPAGQTANAA"
     gene            5301..>5597
                     /locus_tag="AC16PP_08110"
     CDS             5301..>5597
                     /locus_tag="AC16PP_08110"
                     /inference="COORDINATES: protein motif:HMM:NF017911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR6481628.1"
                     /translation="MPLRLSDLQSRLAAGLAPVYFISGDETLLVQEACDAILGTAHSE
                     GFTERSVFHVEKGFRWNDILQDAASLSLFAERRIIDVRVPGNTFDREASEVLRTY"
BASE COUNT          933 a         1747 c         1905 g         1012 t
ORIGIN      
        1 atggagggct gcagcaagta ctgcagcttc tgcgtggtgc cgtacacccg cggagaggag
       61 gtcagccgac cggttgccga cgtgatgcgc gaagtgatcc agctcgccgg tcaaggggtc
      121 cgggaggtga acctgctcgg gcagaacgtg aattcctacc tgggccccat cgacgacgac
      181 catgcagacc tggcggagtt gatctactac gtggccgaag tcgacggcat cgaccgcatc
      241 cgcttcacca cctctcatcc gctggacttc aacgattcgc tcgttcgcgc gttcgccgac
      301 gttccggaac tggtggatca tctgcatctg cccgttcagt ccgggtccga ccgggtgctt
      361 gctctgatga agcggggcca caccgtgagc gagtatcgtg aaaagatcgc ggccctcaaa
      421 gaggtgcgag ggggcatcag cctttcgtcg gacttcatcg tcggcttccc tggcgagacc
      481 gaggcggact tccaggccac catggacctc gtcgccgaga tcgggttcga tacctcgttc
      541 agcttcgtct acagcgcacg ccccgggact ccggcggccg ccctgccgga cgacacgccg
      601 gaagccgtca agcgcgagcg cctggcgctc ctgcagggcc tgctgcgctc tcaggcggat
      661 gccatcgcct gcgacatggt cggaacgcgg cagacggtgc tggtcacggg cgagtccagg
      721 aagaatcggg aagagctgca ggcgcgcacc gagaacaatc gggtcgtcaa cttcccctgt
      781 ccggaccgtc gcctcgttgg cggcttcgtg gacgtggaca ttaccgaagc cctgccaaac
      841 tccctgcggg gcgtgatggc gatcacatag ggcgcagacg ggcgtttggg gcggcgaaac
      901 ccgatttgac aggtaactgc gcgccgatat cctagacaac cctcttctgc acagggcgcg
      961 cattgagcga agacgattca cgggtggtgg cgctcaccac gaatgtcagc ctggaaccca
     1021 acgaaccccg ccgattggca gccttgtgcg ggccgttcga ccagaacctc aaacacctcg
     1081 agaggcggct gggcgtacat attcgcagcc gcggcaacct attccagatc agcgggccgg
     1141 agcagcgtgt tcaggcggcc ggcgcgctgc tgtcccagct ataccgggag acgctcgagc
     1201 acgagaccat cgacccggac atggttcacc tgttccttca ggaatcgggc gtcgacgagc
     1261 tgctcgcgcg tcagcgcggg ccagacgaga tgcccgcgga cggggagggt gcggtcatct
     1321 cgaccgggcg aaaatcagtc aagccccggg gcggcaacca gacccgttac gtcgagcgga
     1381 ttcgcggcca cgatgtgaat ttcggcatcg ggccggccgg aaccggcaag acttacctgg
     1441 cggtggcctg tgctgtgcag gcgttggaaa gccagcaggt gagccggatt ctgctggtgc
     1501 gcccggccgt ggaggcgggc gagaagctgg gctttctgcc cggggatctg gcccagaaaa
     1561 tcgaccccta cctgcgcccg ctctacgacg ccctgtacga gatgatgggc gtggatcgcg
     1621 ttaacaaggc catcgagcgc aacatcatcg aggtggcccc ccttgcctac atgcggggcc
     1681 ggacgttgaa caatgccttc atcatcctcg acgagagcca gaacaccacc atcgcccaga
     1741 tgaagatgtt tctcacgcga atcgggttcg gctccactgc ggtgatcacc ggcgacatca
     1801 cccagatcga cctgcccaaa gggcagaagt cggggctggt caacgtgcgc aacgtgctca
     1861 gcggcatcga cggcatcagc ttcacccact tcggctccaa ggacgtggtg cgccaccccc
     1921 tggttcagaa gatcgtcgac gcttacgagg catatcagga gcctcatgca agcccggaca
     1981 gcgaccgtga tgccggttga agtgcagatc gcatccgtcc aggcggggct tccgtccgtc
     2041 gcgcgcatcg agggctgggc gtccgccgcg ctggacgagc tcggggacga aggcgagccg
     2101 ccggagctgt gtatccgggt cgtcgacgga gaggagtcgc gggagctcaa tgcccgtttc
     2161 cgcggtcatg acaaaccaac caatgtgctg agctttccgg ccgacgtcga tctgccggaa
     2221 gccaagctgc tcggagacgt cgtcatctgc gcgcccgtgg taggcgtcga ggcggccgaa
     2281 cagggcaagt cgccggagga tcacttcgcg cacatggtcg tgcacggggt gcttcatctg
     2341 agcggctacg atcatgagca ggcgggggaa gcggagctca tggaagcgat cgaaagacga
     2401 atactggagc gggtcggcat agccgacccc tacgccgagc gatgagcgag gtcggaggat
     2461 cggagcagca gcggcgttcc ttgctggact ggctcgcaga ccttttcaac gacgagccca
     2521 cggaccgttc cgagctgatg gagacgctgc gcgatgctgc cgaccggcag atcatggata
     2581 gcgaggcgct caacatcatt ttcggggccc tgcaggtgtc ggacatgcag gcgcgggaca
     2641 tcatgattcc gcggacacag ctcgtgtatc tggaggactc caaggccccc gaggagctgc
     2701 tcgccaccat catcgagtct cagcattcgc gctttccggt tgtcggcgat gatctggacg
     2761 acgtcaaagg cattctgcac gccaaggatc tgctgccgct ggcgctctcc agcggctcgg
     2821 gcagcaatgg cttcgacatc aaggactgca tccggccggc aacgatcgtt ccggaaagca
     2881 agcggttgaa cgtcctgctg caggagttca gaagcacccg caaccatatg gcggtcgtgg
     2941 tggacgagta cggtcacgtt tccggcgtgg tgaccatcga ggacgtgctg gagcagatcg
     3001 tgggcgagat cgaggacgag cacgacgtca tcgacgacag cttcgtcaag cagctcgatt
     3061 cccgaacgtt caacgtgaag gcaacgacta ccatcgagga tttcaacgag cacttcgacc
     3121 tcgagctcga cgacgacgag ttcgacacca ttggcggcct cgtgctcaag gcgttcggcc
     3181 acctgcccga tcgcggagag tcggtgcgga tcggagatct cgacttcaaa gtgctcaatg
     3241 ccgattcgcg aagagtcagg ctgctgcagg tcaagctgcg caaatgaagc ccctcctcgc
     3301 gctcgcctgc ggcgcgctgg tccctctctc tcttgcaccc ttcggctggt ggccgcttgg
     3361 gctcgtcgcc gtaggtggct ggtttgcttt gctgcagtcg agtgatcggg gcgggctgct
     3421 tctgggctgg ctgtttggtt taggcaagta cgcggccggc gtatcctgga tctacgtcag
     3481 catcaacgtt tacggcaacg cgccgccgcc gctggccgcc ttcctcgtcg ctctgtttgc
     3541 cggtgggctc gcgctgttcc ccatggtaaa tgcctgggtg ttccagcgcc tgcggaccaa
     3601 gggcgcgccg ctcgtcaacg cctggctgtt cgcctgcctg ttcgtggcgt tcgagtggct
     3661 gctcacctgg tttctgaccg gcttcccctg gctctatccc gcctatggtc acgtcgagac
     3721 ggtcctcggc gggttggccc ccgcgggcgg ggtgatgctg gtgagcctcg ggcttgccgg
     3781 gtcggcctgc tccgtcgccg cggcccatca gtgcgcgcga cggctgcccg tgctcacggc
     3841 ggctctggcg ccctgggttg tcggtgccgt tctgggcgcg gtttcctggg tcgagcccgg
     3901 ggccgttcgc accgtagcgc tcgtgcaggg caacgtggat caggccgtga agtggctgcc
     3961 cgagaaccgg cggcccatca tcgatcgcta ccgggggctg tcggaggacc attggggcgt
     4021 ggacgtcatg gtctggccgg aagccgccat cacggtgatg gcccatgacg ccggggaggt
     4081 tctgggtgat ctggagcgcg agggcgagcg gtcgggcacc gctgtcgtgc tggggctgcc
     4141 ggcggtggag cggcttgccg acgatcggat cgtcctgcgc aacacggcca ttgccttagg
     4201 ggatggatcg gggcgttacg tcaagcggcg gctcgttccc ttcggcgagt acgtcccctt
     4261 ggaggggtgg ctgcgggggc tcatcgagtt cttcgatctg cccatgtcgc gcgctgcgcc
     4321 cggcgattgg cgacagcctc tgctcagggt cggtcaggag caggcggcgc tggccatctg
     4381 ctacgagatc gtctatccgg atctggtcag ggcccaggcc gtcggggcag acgtgctggt
     4441 caccatttcc aacgatacct ggttcggtgc cagcatcggc ccgctgcagc atctgcagat
     4501 ggcgaggatg cgcgctctgg aaaacggccg ctggctgctt cggggaacca acaacggggt
     4561 gaccgccatc gtcgaccacc ggggcagatt ggttgcgcgg ctgcctcagt tcgaggccgg
     4621 cgtgctgcgg ggcgagtacc gactcgtggc aggccgaacg ccctacaacc gtcatggtga
     4681 tgggccggtt ctggtgctgg ttgccttcgg tctcggcgtc gccttctggc gcaggctcgg
     4741 cgcggcgcgt ggcgccgacg ggtagtgtag tatgcggcgc gtgcgcaccc gtctacgcta
     4801 cacctggctg atcccgcctc tgttcctcct catcgggggc tgcggctttc agctgcgcac
     4861 atgggatctc ggcaccagcg tggagagctt ctacgtcagg gacgccagca acaatccgct
     4921 ggcatcgccg cttcggcggg cccttcgtca ggccggcgtc gacgaggcgg gcaacgcttc
     4981 ggatgcccag gtggtggtgc tgctgctgaa cgcccagcgg ggacggcgca gcgtgtcggt
     5041 caccggtcag gctcgggcgg ccgagtacga aatttcgctc ggcgttcagt acggggtgct
     5101 cgacgggcag ggacaggagc tcatcgcgcc gcgctgggtc cgcgggaccc gcgtgttccg
     5161 ggtagaccgg gacaacatcg tcggcaccag cgaggagcag gcgctgctcg agcgggaaat
     5221 ccagaacgat ctcatccagc agatcgtcag ggcgctcaac acggtttccg gcccggccgg
     5281 cccggcgggt cagacggcca atgccgctta ggctgtcgga cctgcaaagc cgtttggccg
     5341 ccggccttgc gcccgtgtac ttcatctccg gtgacgagac cctgctggtt caggaagcct
     5401 gtgacgcgat cctgggcacg gcgcactcgg aggggttcac ggaacggtcc gtgttccatg
     5461 tcgagaaggg attccgctgg aacgacattc ttcaggacgc tgcgagcctg tcgctgtttg
     5521 ccgagcggcg gatcatcgac gtccgggtgc ccggcaacac gttcgaccgg gaagcctcgg
     5581 aggttctgcg tacctac
//
LOCUS       DBNHKV010000254         4581 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1948010, whole genome shotgun sequence.
ACCESSION   DBNHKV010000254 DBNHKV010000000
VERSION     DBNHKV010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1948010"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="AC16PP_08115"
     CDS             complement(<1..579)
                     /locus_tag="AC16PP_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481629.1"
                     /translation="MLVLLSAGNGLAASLEFDDLGEPRSITGEWLYMTGDDPGWADPG
                     LDDGGWLPVTVPGPGIGDHPDAQGMVWYRVHVRFGPPIAADLDARAALGVAIGSVESA
                     YEIYAGGRLLGGMGKLPPEPVAHFDQHGIYPIPLDAVDADGNLVLALRVWRHERAASR
                     SELGHFAGPFRVGSIGDLRAEAVLAAIIPNVLL"
     gene            complement(612..4010)
                     /locus_tag="AC16PP_08120"
     CDS             complement(612..4010)
                     /locus_tag="AC16PP_08120"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR6481630.1"
                     /translation="MNDDSTALSVEARSFTRITVISGLLFVIVGSLALVLAMLYTSPV
                     AWGLAILIDGILLPLSYLSYRWAKQDRRETSATVLAVIWYAIAVGMIIVGERLYGILL
                     VTSILPVLMSLPYVSQVLLRRLILTSVLLVLVGSVGALFPPIITPTVPDDLIAYVESF
                     STASITMMVMLAIWQSAGRLRASADGMREAIAALKESERGLETKVEARTAELRKAVKE
                     MADLNEIAIIVNSTLDLDKVKDSIYQGLQNLFAFEQMGVFLADYDEEKLYLRLQAGAP
                     FDPELSRILVEEGLPLDSSHSFAARSVVKNRSVFLGAITDEGLAMAGVGDRLIYEHNP
                     MKSILMCPLEIQNRAIGCIYFSASREAFELHQNDIESIERYVTQLGTALRNAQLFQEA
                     EEARAEAEAANETKGTFLANMSHEIRTPMNAIIGLTGLCLETELDDKQEDYLRKVDGA
                     ANALRSIIDDILDFSKLEAGKFEFEDIPFSLNDVLDNLATICMVRCQDKHLELVFQRD
                     PGLPDALSGDPTRLGQILINLAGNAIKFTEEGQIVIEARETGRVGDRVSIRFDVRDSG
                     IGMNEEQLSRLFQSFSQADATISRQYGGTGLGLAISQQLSEGLGGHIEVTSEPGVGSS
                     FHFTLEFAVVEAREEGAEREDAPRNLNVLVVDDNEPSRDILREYLESFGYTVTLAESG
                     EEGLALMKPAHDFDLVLLDWMMPGMTGLDVALAIREQPSPPKVILLSSWNMPSTEHQS
                     MVDAFMAKPVKPSTLLDTIMLAYGKQVVRRKRGLGRGMGHEDLAPVRGARVLVVDDSD
                     INLQIACELLQKVPFVLDTASDGAQAVDKVLAHHYDCVLMDIQMPGMDGYTATGIIRE
                     RFSFDELPILAMTANVMAEDRARTRDAGMNGHVAKPVDPAELFQALAEAIPVADYSAN
                     LPEAEDATVVEAAAAPQNEPLPDSLPGLELKQGLARLGGNEDLLLTLLRNLLDEYADC
                     PSDLQRLLETGDEEEARKVAHKLRGIANNLGATGIGKCAEDIERCVLDGRRVDPVHVA
                     DLQEAQQVLATQLAELLASRTTGGGVGNAAEDVDVGAVLSALKSAIAAFDPGAVELVD
                     QLLAGCDPQSNVVQLLEASRQHLDNFNFTDAEPLVAEAEAALLA"
     gene            4103..4579
                     /locus_tag="AC16PP_08125"
     CDS             4103..4579
                     /locus_tag="AC16PP_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6481631.1"
                     /translation="MNADNQKSRVLIVDDEPGNIKILSSVLAGEYALSVATNGEQALQ
                     IAKVQSPEIILLDMIMPGMDGIQVCEALKASEETKDIPVIFVTSMSDTANEERGLEAG
                     AVDYITKPISPPIVKARVKIHIQNYLSMQFLQKLLSDQTGSLDAAKEEAESLLVFV"
BASE COUNT          890 a         1458 c         1410 g          823 t
ORIGIN      
        1 cagcagaacg ttgggaatga tcgctgccaa caccgcctcg gcgcgaaggt ctccgatact
       61 gccgacgcgg aacggcccgg cgaagtgccc gagttctgat ctcgaggcgg ccctctcatg
      121 gcgccagacg cgcagagcga gcaccagatt tccgtcggca tcgacggcat ccaagggaat
      181 cgggtaaatc ccgtgctgat cgaagtgcgc gaccggttcc ggcggcagct tgcccatgcc
      241 accaagcaga cgcccgccgg cgtatatctc gtaggcgctc tccacgctgc caatggcgac
      301 cccgagcgcc gcgcgggcgt cgagatcggc ggcaatcggc ggaccgaaac ggacgtgcac
      361 ccggtaccag accattccct gcgcgtcggg gtggtcgccg attccaggac ctggcacggt
      421 caccggcagc cacccaccgt cgtccaggcc cggatcagcc caaccagggt catcgcccgt
      481 catgtaaagc cactcaccgg ttatcgagcg cggctcaccg agatcgtcga attccagcga
      541 agcggccaga ccgttgccgg cggagagcaa aacaagcacg agggcgcagc accgtcgagg
      601 cagcttcatg atcaggccaa cagggccgcc tccgcttccg ccaccagcgg ctccgcgtca
      661 gtgaagttga agttgtcgag gtgctgacgg gacgcttcga gcagctgcac gacgttactc
      721 tgcgggtcgc atcccgcgag cagctgatcg acgagctcta cagcaccggg gtcgaaagcg
      781 gcaattgcgg acttcagggc ggacagcacg gccccaacgt cgacatcttc ggctgcgttc
      841 cccacgccac cgcccgtcgt tcgcgacgcc agcagctcag cgagttgcgt cgccaggacc
      901 tgctgggctt cctggaggtc cgccacatgg accggatcca cccgccgccc gtcgaggaca
      961 cagcgctcga tgtcctcggc gcatttcccg atgcccgtgg ccccgaggtt atttgctatg
     1021 cccctgagct tatgtgcgac cttgcgcgcc tcttcttcat ctccggtttc cagcaaacgc
     1081 tgcaggtcgg aagggcaatc ggcgtactca tccagcaggt tccgcaggag cgtcagcagc
     1141 aggtcttcgt tgcctccaag ccgggcaagt ccttgcttga gctcgagtcc gggcagggaa
     1201 tccggcagcg gctcgttctg cggagcagcc gccgcctcca cgacagtggc gtcctctgcc
     1261 tcgggaaggt tggcggagta atcggctacc gggatggcct cggccagcgc ctggaacaac
     1321 tcggcagggt ccactggctt ggccacatgg ccattcatgc cggcatcgcg ggtgcgcgcc
     1381 cggtcctcgg ccatcacgtt ggcggtcatg gcgaggatcg gcagctcgtc gaacgagaaa
     1441 cgctcgcgaa tgatgcccgt ggcggtgtag ccgtccattc cgggcatctg gatatccatg
     1501 agcacgcagt cgtagtggtg ggccagcacc ttgtccaccg cctgcgcgcc atcgctggcg
     1561 gtatcgagca cgaagggcac cttctgcagc agttcgcagg caatctggag gttgatgtcg
     1621 gaatcatcca ccacgagcac ccgtgcgccg cgaacgggag ccaggtcttc atggcccatc
     1681 ccccgaccca ggccgcgttt gcgccttacc acctgcttgc cgtaggcgag catgatggtg
     1741 tcgagcaggg tcgacggttt caccggtttc gccatgaacg catcaaccat ggactgatgc
     1801 tcggtactcg gcatgttcca ggacgacagc aggatgacct ttggcgggct cggctgctcg
     1861 cggatcgcga gggccacgtc caggcccgtc atgccaggca tcatccagtc cagcaacacc
     1921 agatcgaagt cgtgcgcggg tttcatgagg gcgaggccct cttctccgct ctccgccaag
     1981 gtcaccgtgt agccgaacga ttccagatac tctcttagaa tgtcccggga cggctcgttg
     2041 tcatccacca ccagcacgtt gaggttcctg ggcgcatcct cccgctcggc cccttcttcg
     2101 cgcgcctcga cgacggcgaa ctcgagagtg aaatgaaagc tgctgccgac accgggctcg
     2161 ctcgtgacct cgatgtgacc gccaaggcct tcgctgagct gctgcgagat ggcaaggcca
     2221 aggccagtgc cgccatactg acgactgata gtggcatcgg cctgtgaaaa agactggaac
     2281 agcctgctca gctgctcctc gttcatgccg atgccgctgt cacgcacgtc gaagcgaatg
     2341 ctcaccctgt cgccaacgcg gccggtctcg cgggcctcga tgacgatctg gccctcctcg
     2401 gtgaacttga tggcattgcc ggccaggttg atgagaatct gaccgagacg ggtcggatca
     2461 cccgacaaag cgtcgggcaa cccgggatcg cgctggaaga cgagctccag atgcttgtcc
     2521 tggcagcgca ccatgcagat ggtggcgagg ttgtcgagca cgtcgttgag cgagaaggga
     2581 atgtcctcga actcgaactt gcccgcttcc agcttggaga agtcgagaat gtcgtcgatg
     2641 atgctgcgca gggcgtttgc cgcaccgtcg acctttctca ggtagtcctc ctgcttgtcg
     2701 tcgagctcgg tttccaggca gagaccggtc aggccgatga tggcgttcat gggcgtgcgg
     2761 atttcgtggc tcatgttggc caggaatgtc cccttggtct cgttggcggc ctcggcctca
     2821 gcgcgggctt cttcggcttc ctgaaagagc tgggcgttgc gcagagccgt gcccagctgg
     2881 gtcacgtagc gctcgatgga ctcgatgtcg ttctggtgca gctcgaacgc ttctctgctg
     2941 gcggagaagt agatacagcc gatggcccgg ttctggatct cgaggggaca catcaggatg
     3001 gacttcatgg ggttgtgctc gtagatgagc cgatccccaa caccagccat ggccaggccc
     3061 tcgtcagtta tcgcgccgag aaagaccgac ctgttcttca ccacggatcg ggctgcaaag
     3121 ctgtgactcg aatcgagcgg caggccttcc tcgacaagga tccgagacag ctcgggatca
     3181 aacggggcac ccgcctgcaa gcgcaggtac agcttttcct catcgtagtc cgccaggaaa
     3241 acgcccatct gctcgaaggc aaacaggttt tgcaggccct ggtagatgct gtctttgacc
     3301 ttgtccaggt cgagggtcga gttgacgatg atcgcgatct cgttgagatc agccatttcc
     3361 ttgaccgcct tccgcaactc ggccgtgcgg gcttcaactt tggtctccag gcccctctcc
     3421 gactccttga gcgcggcgat cgcttctcgc atgccgtcgg cggaagcacg aaggcgcccg
     3481 gcggactgcc agattgccag catcaccatc atcgtgatgc ttgccgtgga aaaggactcc
     3541 acgtaggcga tgaggtcatc gggcaccgtg ggcgtgatta tcggcggaaa cagagcgccc
     3601 acgctgccca ccagcaccag gagcaccgag gtgaggatca gccggcgcag cagcacctgc
     3661 gaaacatagg gcaggctcat gagaaccggc aggatggaag tcaccagcag gatgccgtag
     3721 aggcgttccc cgacgatgat catgcccacc gcaatcgcgt accagatgac ggcgagtacc
     3781 gtcgccgacg tctcccgccg gtcctgcttc gcccagcgat agctgagata cgacaacggc
     3841 agcaggatgc cgtcgatcaa aatcgccagc ccccaggcca ccgggctcgt atacagcatc
     3901 gcgagaacca gggcgaggga cccgacaatg acgaacagca acccgctaat aacggttatg
     3961 cgcgtaaagg agcgcgcttc gacactcagc gcggtggaat catcgttcac ggaaggccct
     4021 atcggtcgtc gtctttatcg atctcggctc gccagtataa tcgagaagtg atagcttcgg
     4081 cgctggcaat ctggagtcgg ggatgaacgc agacaatcag aaaagccgag tgctcatcgt
     4141 ggacgatgaa cccggcaaca tcaaaattct atccagcgtg ctggccggcg agtacgccct
     4201 gagcgtcgcc acgaacggcg agcaggccct gcagatcgcc aaggttcagt caccggagat
     4261 catcctgctg gacatgatca tgccgggcat ggacggcatc caggtctgcg aagctctcaa
     4321 ggcgagcgag gagaccaagg atatcccggt gattttcgtc accagcatga gcgatacggc
     4381 aaacgaggag cgcgggctgg aagccggcgc ggtcgactac atcaccaagc cgatcagccc
     4441 gcccatcgtc aaggcccggg tgaagatcca catacagaat tacctgagca tgcagtttct
     4501 gcagaaactg ctgtcggacc agaccggctc cctcgacgcg gccaaggagg aagccgagtc
     4561 gctgctcgtc ttcgtttgac c
//
LOCUS       DBNHKV010000255         9383 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1029114, whole genome shotgun sequence.
ACCESSION   DBNHKV010000255 DBNHKV010000000
VERSION     DBNHKV010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9383)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9383
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1029114"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..875)
                     /locus_tag="AC16PP_08130"
     CDS             complement(45..875)
                     /locus_tag="AC16PP_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR6481632.1"
                     /translation="MRKFLGRMTRRGIVSLLAGLLVTLPIGVNAAKDQSPVLAQIAKW
                     GKIRVGMSGSQPPYNFVSREGKLIGMDVDIAELLAKSLDVQLEMVQMNFNELLPALES
                     GKVDLVISGMTATLQRNMRAAFVGPYHITGKSILTRSETIAALGSNDLNREDLRIAAL
                     KGSTSEDFVRRVAPNAKLVATDTYDEAIDQLMANKAELFVADAPRVMLEVMRWPDAGL
                     TAAQRPLTIEPIGIAVPASDPLLLNLVENYMAALESSGALEKLEEKWFKSGGWLVQLP
                     "
     gene            complement(996..2012)
                     /locus_tag="AC16PP_08135"
     CDS             complement(996..2012)
                     /locus_tag="AC16PP_08135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HIR6481633.1"
                     /translation="MQAIRCHEISEDIGAVRLETISLDPPGEGEVQVRLKACAVNFPD
                     LLMIQGKYQFKPPLPFSPGTEAAGDVVAVGDGVSAFDVGDRVVVALRYGGFAEAVNVP
                     AESVQPVPGSMSYDKAASYRTAYLTAYVALAKRGDLTPGEVLLVHGATGGVGMAAVDL
                     GKRLGAVVIATGGSDEKLAVVKARGADHVINYTQADGTLGGFREEVKALTDGRGADVI
                     YDPVGGDVFDESMRCINWGGRILTIGFTSGRWPQAPVNLILIKQIAVIGVRAGEYGRR
                     DPAKGREAERALYAMAAAGEIDPYVCAGFPLERAVEAMRMLEQRKVVGKVVVTMNGFD
                     VASP"
     gene            complement(2022..2417)
                     /locus_tag="AC16PP_08140"
     CDS             complement(2022..2417)
                     /locus_tag="AC16PP_08140"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6481634.1"
                     /translation="MIRDAETFEHLLAAVRRFVAEVAVPNEERVEADDEIPAEGVEAM
                     RNLGTFGRSIPEAYGGSGLTSEELALAFMELTRCSVAYRVVQIFGGQGYVKGRGSERF
                     YRDVRAFRIYEGTSQIHLLNIAKQLLKDA"
     gene            complement(2417..3361)
                     /locus_tag="AC16PP_08145"
     CDS             complement(2417..3361)
                     /locus_tag="AC16PP_08145"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6481635.1"
                     /translation="MEIYLHHYPASLFSEKIRLLLGYLGGSWHSVEIPSIMPRPLLMP
                     LSGGYRRTPVLQIGANVYCDTAVIARGLARHFGDEDLFAAGFTANRVAEWADTQLFRV
                     TVALNFRPEAVGGFMSQLSPEEIAAFQKDRAELVGDAPMVSFSPEAAQAYLATYLDQA
                     DRALDGGFLFGGKPCIADFCFYHCLWFLENNPLNAPLLDAYGNVRGWMQTMAGFGHGS
                     VAPSSGEAALEHARSCSPVAPVLESPLAAPFELDVGRRVTVTPADYGRVPVAGSLVAW
                     DCEEVVILREDAATGPIMNHFPMAGFQVAPAGNLGSSG"
     gene            complement(3446..4771)
                     /locus_tag="AC16PP_08150"
     CDS             complement(3446..4771)
                     /locus_tag="AC16PP_08150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR6481636.1"
                     /translation="MMRDTSLTGLAESLVAYGTRRGADQVEVAIRTGSNFSASIREGE
                     LETLVEAGFRELEVKLIVDQKVATEASSDLSEATLKRLIDNGIGRARLSGADPFAGLP
                     EKEPVKVRAEALDIFDEAVVDMSPESKIAAARRVEAICLGDKRIRRSMGAGFGTTIET
                     LYLANSNGFSDAFRQTACSNGVYLQVGEPDNLISEGKYDYSRNLEGLRSPEDIAAEAI
                     DRSTRLIGARKVPTQNVPVVLEPEMSAELLQFLASCVDGRGVYRKQSFLADRIGERVA
                     GANVNVFDDGLMPGAPGAKPFDREGVPLRKTAVIEKGVLKSFLLDSYSARKLGMKPTG
                     NGAGPNNLYLASGKDSPADIIGSVDEGLLLTGSIGFGAVPTTGDISRGAFGIWIEGGE
                     LAYPVAGITIGANLGTLLQQVEMIGDDLDHRRYVNGPTIKVAEMTIGGT"
     gene            complement(4768..6291)
                     /locus_tag="AC16PP_08155"
     CDS             complement(4768..6291)
                     /locus_tag="AC16PP_08155"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR6481637.1"
                     /translation="MRADRRDFLKKTGSAVAAASTASLWPDLLLAEEVPTTAEALFET
                     RFGVSRGDMGRLLSLALGKGGEFAELFFEYKIANGVTMEEDIVKESSERVSLGVGIRV
                     LKGEQTGYGYTSDLTFEGMSQAARTAAAIASEGGRVEIAPLRLRANALQVYDLERPFA
                     ESSLSSRMILLRQTYDAAQAYHPKIEKVRVSLRDEIQYVLVANSEGLLASDTRPQVRL
                     IASVTAADGGNRSTGLSTAGGRVGSAFFSSARTPKELGREAAEEAIVLLGAVSPRPGD
                     QTVVLDSGQSGVMIHEAVGHPLEADSNRKKRSVFWDKLNQTVANPLVTIYDDPTIAHF
                     RGSHNMDDEGTLPRKTMLIEKGKLVGYLQDRLSAKLMKMSPSGHGRRDGFENVPLPRM
                     ANTNLARGESDPEQIVRSVKRGFFAKTYQGGMVQPSGKFTFSVNMGYLIEDGKLTAPV
                     KNATLIGSILDVLPEVTMVGNDMGTFLGTCGKGGQSAPVTAGTPTLKINSMTVGGRS"
     gene            complement(6360..8003)
                     /locus_tag="AC16PP_08160"
     CDS             complement(6360..8003)
                     /locus_tag="AC16PP_08160"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR6481638.1"
                     /translation="MLRVLRFSLPVAVAVLAFAVPLRVGAEVVAFELREFFDDYYSSG
                     AMAVQGQALLTERVLAEFYARRGYRPAWGDPALVAELFDLLARSVDDGLDPDDYNLTR
                     LRALRERAASSPKAVPERDLELLYTSSLLRFAYHQRFGKVNPKTLVSTWNFGRQLAPG
                     VDAADTMNQIVESGSLREGLAARVPRGPLYRNIQMALARYRSAAAAGGWEPVPEGVTL
                     TIGMRDERVVSVRRRLAVTGDLETGAVSDPELFDADLESGVRRFQHRHSLSVDGAVGP
                     ATVRAMNVSVEERIDQLRLSLERARWIMTARDTSVVVVNIAAAEVYVLEEGEMVWQRR
                     AMVGRTYRQTPVFRGLMTYLEINPTWTVPPGILRADILPKVREDPGYLARSNMTLLDR
                     SGRAVDPYAVDWQALEGMPYIFRQEPGPTNALGLIKFMFPNEHFVFIHDTPNRELFER
                     AERTFSSGCIRVEDPFSLAALLLRDPANWDEAAVRAAVETGETRRVRLADPWPVYILY
                     WTAQVDDDGVVRFYPDVYQRDVELLGELDGDVRIDLPAS"
     gene            8177..9100
                     /locus_tag="AC16PP_08165"
     CDS             8177..9100
                     /locus_tag="AC16PP_08165"
                     /inference="COORDINATES: protein motif:HMM:NF019896.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HIR6481639.1"
                     /translation="MKWLICLLVAGFSLPAAAYREATVPFGVKVQGIENRFAEFGVYA
                     RPGEILALDLIDVAPEEIGATFDSAPVKTGHGRINLKMPGAPGTHRVELTDQRSGSRM
                     RLNVFVMWQHDGARDDVLNGYRIGQYPRDPFRGLAVYRPPEAFVEVTPDNAHTRVSPN
                     FTLGQFVCKQGSGFPKYVVLRPQLVQKLELVLGELNAVNPDVTGLTVMSGYRTPAYNR
                     SLGNGRHSRHLWGGAADVYVDANGDGIMDDLNGDGRRDRGDAEWLATFIDRLEHAGRF
                     GEHIGGIGTYNSNAHHGPFVHVDARGYRARW"
BASE COUNT         1727 a         3187 c         2855 g         1614 t
ORIGIN      
        1 ccgaaggaat cggcgcgaaa ccaggaccac gggttcatct caggtcacgg cagctgaacg
       61 agccaaccgc cgctcttgaa ccacttttct tcgagcttct ccaaggcgcc gctcgactcg
      121 agagcggcca tgtagttctc caccaggttc agcagcaacg ggtcgctggc gggaaccgcg
      181 atgccgatgg gctcgatggt cagcggacgc tgggccgcgg tcaggcccgc atccggccac
      241 cgcatgacct cgagcatgac tcggggagca tcggcgacga acagctccgc cttgttggcc
      301 atcagctggt cgatggcctc gtcgtaagtg tccgtggcca ccaacttggc attcggagcc
      361 acccgccgca cgaaatcctc gctggtcgag cctttcagcg cggcgatgcg caggtcctcg
      421 cggttcagat cgtttgagcc cagggcggca atggtttccg agcgcgtcag aatggacttg
      481 ccggtgatgt ggtaaggccc gacaaacgcg gcgcgcatgt tgcgctgcaa cgtggccgtc
      541 ataccggata tcaccagatc gaccttgccg gactccaatg ccggcagaag ctcgttgaag
      601 ttcatctgca ccatctcgag ctgaacatcg agcgacttgg ccagcagctc ggcgatgtcc
      661 acgtccatgc cgatgagctt gccctcccgg ctgacgaagt tgtacggcgg ctggctaccg
      721 gacatgccga cgcggatctt gccccacttg gcgatctgag cgagaacggg cgactgatcc
      781 ttggcggcat tgacgccgat ggggagagtg accaggaggc cggcgagcag actgacaatg
      841 ccccttctgg tcatacgtcc gagaaactta cgcatgggtc ttccttagtt gattggtttc
      901 catatttctt gacgcccgcc ttcaaacggg cgggtcgaac ggggaggccg ttatacaaca
      961 agccaggcgc gacgaacagc ctctcggcgg aggagctatg gtgaggcgac atcaaagccg
     1021 ttcatggtga ccaccacctt gccaaccacc ttgcgctgct cgagcattcg catggcctct
     1081 accgcccgct ccagcggaaa acccgcgcag acgtacgggt cgatctcgcc ggcggccgcc
     1141 atggcataca gtgcccgctc ggcctcgcgc cccttcgcgg gatcccgccg cccgtactcg
     1201 ccggcgcgaa cgccgatcac ggcaatctgc ttgatcagaa tcagattaac gggcgcctgg
     1261 ggccagcgcc cgctggtgaa cccgatggta aggatgcggc cgccccaatt gatgcagcgc
     1321 atggattcat cgaacacgtc gccgccaacg ggatcgtaga tgacgtcagc cccccgcccg
     1381 tcggtcagcg cctttacttc ctcgcgaaac ccgcccagcg tcccatcggc ctgggtgtag
     1441 ttgatcacat gatccgcgcc acgggccttg accaccgcca gcttctcgtc gctcccgcca
     1501 gttgcgatga ccacggcacc cagacgtttg cccagatcca ccgccgccat gcccacacct
     1561 ccggtggcac cgtgaacgag cagcacctcg ccgggcgtca ggtcgccacg cttggccagc
     1621 gccacgtagg cggtgaggta ggccgtccgg tacgaagccg ccttgtcgta gctcatcgag
     1681 ccgggaacgg gctgcacact ctccgcgggc acattcaccg cctccgcgaa accgccgtag
     1741 cgcagcgcca cgaccacccg gtcgcccacg tcgaacgcgc tcaccccatc gccgacggcg
     1801 acgacgtcac cggcggcttc cgtgccggga gaaaacggca ggggcggctt gaactgatac
     1861 tttccctgga tcatgagcag atcgggaaag ttgaccgcac aggccttcag gcgcacctga
     1921 acctccccct cacccggcgg gtcgaggctg atggtttcga gacggacagc gccgatgtcc
     1981 tcggagatct cgtgacagcg gatggcttga atatcggtca cctaggcgtc cttgagcagt
     2041 tgcttggcaa tgttgagcag atgaatctgg ctggtaccct cgtagatccg aaacgcgcga
     2101 acgtcccgat agaaccgctc gctgccgcgg cccttgacgt agccctgccc accgaagatc
     2161 tgcaccaccc ggtaggctac cgagcaacgg gtcagctcca tgaaggcgag cgcaagctcc
     2221 tcgctggtga gaccgctgcc gccataggcc tcagggatgc tccggccgaa ggtaccgaga
     2281 ttacgcatcg cctccacgcc ctccgccggg atctcatcgt ccgcctccac acgctcctcg
     2341 ttgggaacgg caacctccgc gacgaatcgg cgcacggctg ccagcagatg ctcgaaggtt
     2401 tctgcgtccc tgatcatcag cccgacgacc ccagattgcc ggcgggagcc acctgaaagc
     2461 ctgccatcgg aaagtggttc atgatcggcc cggtagccgc gtcctctcgc aagatcacga
     2521 cctcctcgca atcccaggca acgagggatc cggccacggg cactcgtccg tagtcggccg
     2581 gcgtcacggt gacacgccga cccacatcga gttcgaaggg agccgcaagc gggctctcca
     2641 gtaccggggc gacgggactg caggaccgcg cgtgctcgag cgcagcctcg ccgctgcttg
     2701 gtgcaacgga tccgtgaccg aagcccgcca tggtctgcat ccagccgcga acgttcccat
     2761 aggcgtccag cagaggagcg ttcagcggat tgttttccag aaaccacaga caatgataaa
     2821 agcagaagtc ggcgatgcag ggcttcccgc cgaacaggaa cccgccgtcg agcgcccggt
     2881 ctgcctggtc gaggtaggtg gccagatagg cctgcgccgc ttcaggggag aacgagacca
     2941 tcggcgcatc acccacgagc tcggcccggt ccttctgaaa ggctgcaatc tcttccgggg
     3001 aaagctggct catgaaaccg ccgaccgcct ccggccggaa gttgagcgcg accgtcacgc
     3061 ggaacagttg ggtatccgcc cactccgcga cccggttggc cgtaaagccg gccgcaaaca
     3121 gatcttcgtc gccgaaatgc cgggcgagcc ccctggcaat gaccgcggta tcgcagtaga
     3181 cgttggcacc gatctgcagc acgggcgtgc gtcgatagcc tcccgacagc ggcatcagca
     3241 aagggcgcgg catgatgctc ggtatctcga ctgagtgcca ggaaccgccc aggtacccca
     3301 gaagcagccg gatcttctcc gaaaacaggg aagccggata gtggtgcagg taaatttcca
     3361 actgttcccc cagaacggac cttcgcggta cgcgcgcgac cactgcgttt gccgcccggg
     3421 acacacgcgt gctccgtacc cggcctcagg tcccgccgat ggtcatctcc gccaccttga
     3481 tggtcgggcc gttgacgtag cggcgatggt ccaggtcgtc gccgatcatc tccacctgct
     3541 gcagcagcgt cccaagatta gcaccgatgg tgattccggc aaccgggtaa gcgagttcgc
     3601 ccccttcgat ccagatgccg aaggcgcccc gggagatgtc gcccgtggtg ggcaccgccc
     3661 cgaacccgat gctgcccgtg aggagcaggc cctcgtccac tgaaccgatg atgtccgcag
     3721 gagaatcctt tcctgacgct agatagagat tgttcggccc cgccccgttc cccgtgggct
     3781 tcatccccag ctttcgagcg gagtaggagt cgagcaggaa gctcttgagc accccttttt
     3841 caatcaccgc cgtcttgcgc agggggaccc cctcgcggtc gaagggcttc gcgcccggcg
     3901 cgcccggcat cagaccgtcg tcgaatacgt tcacgttcgc cccagccacc cgctcgccga
     3961 tccggtcggc cagaaagctc tgcttgcgat acacaccgcg gccatcgacg cagctcgcga
     4021 gaaactgcaa cagctctgcg ctcatctccg gctccaggac cacgggcacg ttctgagtag
     4081 gaaccttgcg ggcgccaatc aggcgggtag atcgatcgat ggcctccgcc gcgatgtcct
     4141 ccgggctcct cagcccttcg aggttacggg agtagtcgta ctttccctcg ctgatgaggt
     4201 tgtcgggctc ccccacctgg aggtacacgc cattggaaca ggccgtctgc cggaacgcgt
     4261 cgctgaaccc gttggaattc gcgagataca gcgtctctat ggtggtgccg aagccggcac
     4321 ccatggatcg cctgattcgt ttgtcgccaa ggcaaatggc ctccacgcgg cgtgcagccg
     4381 cgatcttgct ctccggagac atgtcgacca ccgcctcgtc gaatatgtcg agggcttcgg
     4441 ctcgtacctt gaccggctcc ttctccggca accctgcaaa cgggtccgcg ccggacaacc
     4501 gcgcccgtcc gatcccgttg tcgatcagcc gcttcaacgt cgcctccgac agatcggatg
     4561 acgcctccgt ggcgaccttc tggtcgacga tcagcttcac ctcgagctcc cggaaacctg
     4621 cctccaccag ggtttccagc tcgccttccc tgatggacgc agagaagttg ctgcccgtgc
     4681 ggatggcaac ctcgacctga tccgcaccac gccgggttcc gtaagcaacc aacgactcgg
     4741 cgagccccgt caggctggtg tcgcgcatca tgagcggcca cccacggtca tgctgttgat
     4801 cttcagagtg ggcgtgcccg ccgtcacggg cgcgctctga ccgcccttgc cgcatgttcc
     4861 caggaacgtg cccatgtcgt tgcccaccat ggtcacctcg ggaagcacgt ccagaatgct
     4921 gccgatcagc gtggcgttct tgaccggcgc ggtgagcttg ccgtcctcga tgaggtaacc
     4981 catgttgacc gagaaggtga acttgcccga aggctgcacc atgccgccct ggtaggtctt
     5041 ggcgaagaac ccgcgtttga cggaccggac gatttgttcc gggtcggact ctccgcgcgc
     5101 gaggttcgtg ttggccattc gcggcagcgg gacgttctcg aaaccatcac gacggccgtg
     5161 gccgctcggc gacatcttca tgagctttgc cgagagacgg tcctgcaggt agccgaccag
     5221 cttgcccttc tcgatcagca tggtctttcg gggcagcgtg ccttcgtcgt ccatattgtg
     5281 gctgcccctg aagtgcgcaa tggtgggatc gtcgtagatg gtgaccaggg gattggcgac
     5341 ggtctggttc agcttgtccc agaacaccga ccgctttttg cggttcgagt ccgcttccag
     5401 cgggtggccg accgcctcgt gaatcatgac gccggattga ccggaatcga gcaccaccgt
     5461 ctggtccccc ggtcgggggc tgaccgcacc gagcaacacg atggcctcct cggccgcctc
     5521 ccgaccgagc tccttcggcg tgcgcgccga actgaaaaaa gcggatccca ctcgcccacc
     5581 ggccgtcgac agtcccgtgc tgcgattgcc tccgtcggcc gcggtgaccg aggcaatgag
     5641 tcggacctgc ggccgcgtat ccgatgccag caggccctcg ctgttggcaa ccaggacgta
     5701 ctgaatctcg tctctcagag acacccgcac cttctcgatc ttcgggtggt aggcctgagc
     5761 ggcatcgtac gtctgcctaa ggaggatcat tcggctgctc agggaactct ctgcaaacgg
     5821 tcgttcgagg tcgtagacct gcaaggcgtt ggcgcggagc ctcaacggtg caatctccac
     5881 cctgccccct tcagaggcga tcgccgcggc cgtgcgcgct gcctgactca tgccctcgaa
     5941 ggtgaggtcg ctcgtgtacc cgtagccggt ctgctctccc ttgagcaccc ggatgccgac
     6001 accgaggctg acccgctccg aggactcctt gacgatgtcc tcctccatgg tgaccccgtt
     6061 ggcgatcttg tactcgaaga acagctcggc aaactcgccc cccttgccga gcgccagcga
     6121 caggagcctg cccatgtcgc cacggctcac gccgaagcgc gtttcgaaca gggcttctgc
     6181 cgtggtgggc acctcctctg cgagcagcag gtcgggccaa agggacgccg tggaggcggc
     6241 cgcgaccgca gagcccgttt tcttcagaaa gtcgcgtctg tctgcccgca ttgtgacgag
     6301 ttctcctttt tcactgatgt agcgccctca gtcggcagca tcgctcgaac cagccgggtt
     6361 caggaggcgg gaagatcgat ccgaacgtcg ccgtcgagtt ccccaagcag ctccacgtcg
     6421 cgctgataca catccgggta gaagcggacg actccgtcgt cgtccacctg ggcggtccag
     6481 tagaggatat agacgggcca cggatccgca aggcggaccc gccgggtctc accggtttcc
     6541 accgcggcgc gcaccgcggc ttcgtcccag ttcgccggat cgcgaagaag cagggcggcg
     6601 agggaaaacg gatcctccac cctgatgcag cccgagctga acgtacgctc cgcccgttcg
     6661 aagagctcac ggttcggggt gtcgtggatg aagacgaagt gctcgttggg aaacatgaac
     6721 ttgatcaggc ccagcgcgtt cgtcgggccc ggctcctgcc ggaagatgta aggcatgccc
     6781 tcgagagcct gccagtccac ggcgtagggg tcgacggcgc gacccgatcg gtccagcagc
     6841 gtcatgttgg agcgggccag atatccagga tcctcgcgca ccttcggcag gatgtccgcc
     6901 cgcagaattc cgggcggtac cgtccaggtg gggttgattt ccagataggt cataaggccg
     6961 cgaaacaccg gcgtctggcg ataggtccgg ccgaccatgg cccgacgctg ccagaccatc
     7021 tcaccttctt ccagaacgta gacctccgcg gccgcgatgt tcacgacgac caccgacgtg
     7081 tcccgcgcgg tcatgatcca tcgcgccctt tcaagggaca ggcgcagctg gtcgatccgc
     7141 tcctccacgg agacgttcat ggcgcgcacc gtagcgggac caacggcccc gtccaccgac
     7201 aggctgtggc gatgctggaa ccgcctgaca ccgctttcca ggtcagcgtc gaacagttcc
     7261 gggtcggaca ctgcgcccgt ctcgagatcg cccgttacgg ccagccgccg gcgaacgctg
     7321 acaacccgct cgtcgcgcat gccgatggtc aatgtgacgc cctcggggac gggctcccat
     7381 ccccccgccg cggcggcgga ccggtacctc gcgagcgcca tctggatgtt acggtagagg
     7441 gggccacgcg ggacgcgagc agccagccct tcgcgaagac tccccgactc cacgatctgg
     7501 ttcatggtgt ccgcagcatc gacgcccggc gcgagctgcc ggccaaagtt ccacgtgctc
     7561 acgagcgtct tcggattgac cttgccgaac cgctggtgat aggcgaaacg caaaaggctt
     7621 gaggtgtaca gaagctcgag gtcgcgctcc gggactgcct tcgggctcga tgccgcccgt
     7681 tcgcgcaagg cgcgcaaacg cgtgaggttg tagtcgtcgg gatcgaggcc gtcgtccaca
     7741 ctccgggcca ggagatcgaa cagctcggcg acgagcgccg ggtcacccca cgccggccga
     7801 tagccccgac gagcgtaaaa ctcggccagc accctctcgg tcagcagggc ctgcccctgg
     7861 accgccatgg caccgctgct gtagtagtcg tcgaaaaatt cccgcagctc gaaggcgacc
     7921 acctccgcgc cgacgcgaag cggcacggcg aaggcaagaa cggcgactgc caccggcagg
     7981 ctgaaacgga gcactcgaag catgattccg ggccggatca acgggcgagc caaggtagca
     8041 atctccacac agcagtacaa actgcaggct tgccgggcgg gcgccgggaa ctttagagcg
     8101 ccaaacggat cctatattgt tggacaatcc agggcgtgag tccgtccaaa ctcccgaaaa
     8161 acagagacgc tccccgatga aatggctgat ctgcctgctt gttgccggat ttagccttcc
     8221 ggccgccgcc tatcgtgaag ccacggtacc tttcggcgtg aaggttcagg gaatcgagaa
     8281 ccgattcgcc gaattcggcg tgtacgcgcg gcctggagag atcctggctt tagaccttat
     8341 cgacgtcgcc cccgaagaga tcggcgcaac cttcgactcg gcccccgtta aaacgggtca
     8401 cggccggatc aacctcaaaa tgccaggcgc gccgggaacc catcgggtgg agctgaccga
     8461 ccagcgcagc gggtccagga tgcggctcaa cgtcttcgtg atgtggcaac acgacggggc
     8521 gcgtgacgac gtgctcaacg gttaccgcat cggtcagtac cccagggatc ctttccgcgg
     8581 tctggccgtc taccgcccgc ccgaggcgtt cgtcgaggtc acccccgaca acgcgcacac
     8641 gcgcgtctcg ccgaacttca ccctggggca gttcgtctgc aagcagggtt cgggattccc
     8701 caagtacgtg gtcctgcgcc cacagctggt gcagaagctg gagctcgtgc tcggtgagct
     8761 gaacgccgtc aacccggacg tcacgggcct gacggtgatg agcggctacc gcaccccggc
     8821 ctacaacaga tccctgggca acggccggca cagccggcac ctttggggcg gcgccgccga
     8881 cgtctacgtg gacgccaacg gcgacggcat catggacgat ctcaacggcg atggccggcg
     8941 cgaccgcggc gacgccgagt ggctggcgac cttcatcgac cgactggagc acgcagggcg
     9001 cttcggcgag cacatcggcg gcatcggcac ctacaacagc aacgcccacc acggcccctt
     9061 cgtccacgtc gacgcgcgcg gttaccgggc tcggtggtga tcggaaaaaa gtgggaacct
     9121 cttgccccga gttcaggtgg caacggcgac aaccccgcct gactcccgat cctcctgatg
     9181 accctgcaac ggatctgcct cgacacgaca ggcgtgtcgt acccgataca cttggacgtc
     9241 cggccctttg acctcccagg gggtcgaccg cgcgtccaga acaagctcaa atctcgagtc
     9301 ctcctgtaga cccccataga agacaggatg cgcagcctga atccgcgtac ctgccgagat
     9361 cacgactagt tcgaaacatt cat
//
LOCUS       DBNHKV010000256         2983 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1806781, whole genome shotgun sequence.
ACCESSION   DBNHKV010000256 DBNHKV010000000
VERSION     DBNHKV010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1806781"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="AC16PP_08170"
     CDS             complement(<1..321)
                     /locus_tag="AC16PP_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012336.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6481640.1"
                     /translation="MEDVYIVEAVRSPIGKRGKGLAGLMPADLLGAVQKAALERSGIA
                     PEKVGQVVGGCVSQVGEQSFNIARIAWLSQGLPEEVAATTVDSQCGSSQQATSLGAAM
                     VASGM"
     gene            581..982
                     /locus_tag="AC16PP_08175"
     CDS             581..982
                     /locus_tag="AC16PP_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015095134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HIR6481641.1"
                     /translation="MTNAIIGGLLIGAAAVWLWISLGRIAGISGIAAQALTRPRQSLW
                     PLLFVAGLGIGGWLGQGVRHPEAIPELGVDSIVLLTLGGLLVGFGTRLGSGCTSGHGV
                     CGIARFSRRSLAATVIFVGMGMLTATLVHGT"
     gene            991..1416
                     /locus_tag="AC16PP_08180"
     CDS             991..1416
                     /locus_tag="AC16PP_08180"
                     /inference="COORDINATES: protein motif:HMM:NF042761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HIR6481642.1"
                     /translation="MSALLAGVIFGLGLSISGMADPTIVLAFLTLGPGWNASLLVVMA
                     AAVTVTTIGYRLAGRRPAPLLDSEFHAPARRDIDQRLVIGAVVFGVGWGLAGYCPGPA
                     IVGALTLDPRALAFLPGFVVGMIAFEAVPKPSARQPEQA"
     gene            1460..1837
                     /locus_tag="AC16PP_08185"
     CDS             1460..1837
                     /locus_tag="AC16PP_08185"
                     /inference="COORDINATES: protein motif:HMM:NF015953.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR6481643.1"
                     /translation="MIGIILRILITALGLWAAASIIPGVQIDSTGTMVWAAIWLGVVN
                     AVIRPVAIILTLPLTLISLGGFLLVVNAAMFGLVAAMLNDFHVSGFFAALFGALIVSI
                     TSWLGSSFIGADGRYEVMVIRRD"
     gene            1928..>2983
                     /locus_tag="AC16PP_08190"
     CDS             1928..>2983
                     /locus_tag="AC16PP_08190"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6481644.1"
                     /translation="MQRNFITLISALMALIGLLCNPGVADAKQLLWGDTHLHTSYSFD
                     AFLNNNLTADPDTAYRFAKGEPVIHPYNRARVQLQKPLDFLVVSDHAEFIGGMLHVYR
                     NGLDLDDAGIITQIEGWYRERQLREAIDAGEGAPVFRSLLPAGSDDPRTAAATFGQSQ
                     AQQLPVSPEVFRSAWQEIVDTAEAHYQPGKFTSLIGWEWSSVPGGANLHRIVVTDADA
                     QTAKTFQPFSSLESPYPDDLWAWLEKTSAATSAAFIAIPHNSNISKGWMFPRETLRGG
                     PVDAAYGTTRMRWEPVVEATQIKGDSETHPLLSPNDEFADFEEYSFYIEQQPGEYKPG
                     EGDYVRPALKTGLEIGAE"
BASE COUNT          485 a         1028 c          937 g          533 t
ORIGIN      
        1 catgccggag gccaccatgg ccgcccccag ggacgtggcc tgctggctgg agccgcactg
       61 ggaatcgacg gtggtggccg ccacttcctc gggcagcccc tgggacagcc aggcaatccg
      121 ggcgatgttg aacgactgct cgcccacctg ggacacgcag ccgcccacca cctgcccgac
      181 tttctccggc gcgatgccgc tgcgctccag cgccgccttc tgaacggctc cgagcagatc
      241 ggccggcatc aggccggcaa gccccttacc acgcttgccg atggggctgc ggacggcctc
      301 gacgatgtag acgtcttcca ttgcttacga actccctgga cgagagcgcc gcattctacc
      361 cgagccaggg cagctcgcca cgggtttccg gacgctcagc ttcgcgaacc ccgatcctca
      421 atcagaattt ccgcaccgtc agtcgtctgc actccgcaga tcctctctag atcctggcca
      481 cactttacta ccgggactca tcccgggtta cgccgggaac taggcaggtt gcgctcttcg
      541 tggcaagatc gcccaccctc acgtaaccga ccggaaagtc gtgacaaacg caatcatcgg
      601 cgggctgctc atcggcgccg cggcggtgtg gctctggatt agcctcggcc gcatcgcagg
      661 gatcagcgga attgccgccc aggcgctgac gagaccgcga cagagcctct ggccgctcct
      721 gttcgtcgcc ggcctgggca tcggcggctg gctggggcaa ggcgtccgtc accccgaggc
      781 gatcccggaa ctcggcgtcg acagcatcgt cctgcttaca ctgggcgggc ttctggtcgg
      841 cttcggcacc cgcctgggtt cgggctgcac cagcggtcac ggcgtttgcg ggatcgcccg
      901 cttctccaga cgttccctgg cagccaccgt catcttcgtc ggcatgggca tgctgacggc
      961 taccctggtg cacggaacat gatggggccc gtgtcagccc tgttggccgg agtgatattc
     1021 gggctgggtc tgagcatctc cggcatggcg gaccccacca tcgtgctcgc gttcctgact
     1081 ctgggtccgg gttggaacgc ctcgctgctc gtcgtcatgg cggccgccgt gacggtcacc
     1141 accatcggct accggctggc gggaagacga ccggcaccac tgctcgacag cgaatttcac
     1201 gcgcccgcgc ggcgggacat cgaccagcgg ctcgtcatcg gcgccgtggt gttcggcgtc
     1261 ggctgggggc tggccggtta ctgtccgggt cccgccatcg ttggagccct gacgctggac
     1321 ccgagggcgc tcgccttcct gcccggcttc gtggtgggga tgatcgcctt cgaggccgtg
     1381 ccgaagccat ccgcccggca gccggaacag gcctgaaggc ccgctggccg tcgccgcccg
     1441 tagggcgcag aatgagacca tgattggcat cattctcagg atcctaatca ccgctctcgg
     1501 tctttgggcg gccgccagca tcattcccgg ggtgcagatc gacagcactg gaaccatggt
     1561 ctgggcggcc atctggctcg gggtggtgaa cgccgtcatc cggccagtgg ccatcatcct
     1621 caccctgccc ctgacgctca tctccctggg cggctttctg ctcgtcgtca acgcggccat
     1681 gttcggtctg gtggcggcca tgctcaacga cttccacgtc agcggtttct tcgccgcgct
     1741 gttcggagcg ctcatcgtga gcatcaccag ctggctgggc tcgagcttca tcggggccga
     1801 cggtcgctac gaggtgatgg tcatccgacg ggattgaccc gcgccacccg gttcgacaag
     1861 gctcatcccc ctggccgata atccgccgcg gtcagatgat gctcaagttc ccggagagat
     1921 tcagccgatg cagcgaaatt tcattactct gatctccgcc ctgatggcac tgatcgggct
     1981 tttgtgcaac ccaggtgtgg ccgacgcgaa gcagctgctc tggggcgaca cccacctcca
     2041 tacgtcgtac tcgttcgacg cgttcctgaa caacaacctc acggccgacc cggatacggc
     2101 ctaccggttc gcgaagggcg aaccggtcat tcatccctac aaccgggcac gggtgcagct
     2161 tcagaaaccg ctcgatttcc tcgtcgtctc cgaccacgcc gagttcatcg gcgggatgct
     2221 ccacgtgtat cgcaatggcc tggacctgga tgacgcgggc atcatcacgc agatcgaggg
     2281 ctggtaccgc gaacggcagt tgcgggaggc cattgatgcc ggcgagggcg cgcccgtttt
     2341 ccgctcgctt ctgccagccg gatccgatga tccgcgcacc gcagcggcca cgttcggcca
     2401 gagccaggct cagcagctgc ccgtatcccc cgaggtgttc cgcagcgcct ggcaggagat
     2461 cgtcgacacc gcggaggcac attaccagcc gggcaagttc accagcctga tcggctggga
     2521 atggagctcg gtgcccggcg gcgccaacct ccaccgtatc gtggtgacgg acgccgatgc
     2581 gcagacggca aagacctttc agcctttcag ctccctggag agcccttatc cggacgacct
     2641 ctgggcgtgg ctggagaaga cgagtgctgc gacctcggcg gcgttcatcg ccattcccca
     2701 caactcgaac atctcgaaag gctggatgtt cccccgggaa accctgcgcg gaggcccggt
     2761 tgacgcggcg tacggcacca cacggatgcg ctgggagccc gtggtggaag ccacccagat
     2821 caagggcgac tcggagaccc accctctgct ctcgcccaac gacgagttcg ccgacttcga
     2881 ggagtacagc ttctacatcg agcagcagcc gggcgagtac aagcccggtg aaggcgacta
     2941 cgtccgcccc gcgctgaaga cggggctgga gatcggcgct gag
//
LOCUS       DBNHKV010000257         5335 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_108894, whole genome shotgun sequence.
ACCESSION   DBNHKV010000257 DBNHKV010000000
VERSION     DBNHKV010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_108894"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="AC16PP_08195"
     CDS             <1..227
                     /locus_tag="AC16PP_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR6481645.1"
                     /translation="ERLGISGGVRVVEVSGAAEEAGLRPGDIITRMNNREVADPASLR
                     EIADELPSGRSVPVLVIRGQVPTFLPLRVP"
     gene            306..2099
                     /gene="lepA"
                     /locus_tag="AC16PP_08200"
     CDS             306..2099
                     /gene="lepA"
                     /locus_tag="AC16PP_08200"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017701262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR6481646.1"
                     /translation="MSSVASIRNFSIIAHIDHGKSTLADRLIQLCGGLADREMEEQVL
                     DSMDLERERGITIKAHSVTLSYKALDGIDYKLNFIDTPGHVDFSYEVSRSLAACEGAL
                     LVVDAAQGVEAQSVANCYTAIDQGLEVLPVLNKIDLPQAEPERVAAEIEDIIGLSAHD
                     VLCVSAKTGSGVPELLEQLISRIPPPEGDPEAPLQALIVDSWFDNYLGVVSLVRVVHG
                     RITKGDRIVAKSVGKAHIVDEVGCFTPKRSGRESLGAGEVGYVVAGIKEIQGAPVGDT
                     IVNAKHQEVPQLPGFSRIKPQVYAGLFPINSDDFESFREALEKLKLNDAALFYEPESS
                     DALGFGFRCGFLGMLHMEIVQERLEREYDLDLISSAPTVVYEVVGADGQLLHIDNPSR
                     LPANYQEIREPIAEVHILVPQDYVGSVMSLCIERRGTQKKMHYSSGQVQMTWEMPLSE
                     VVMDFFDRLKSVSRGFASLDYGFDRFEASNLVRLDILINGERVDALVLIAHRDQAHSR
                     GRLLAEKMKELIPRQLFDVAVQAAVGGQIVARQTVKALRKNVTAKCYGGDVTRKKKLL
                     EKQKAGKRRMKQVGRVEIPQEAFLAALKVER"
     gene            2112..2927
                     /gene="lepB"
                     /locus_tag="AC16PP_08205"
     CDS             2112..2927
                     /gene="lepB"
                     /locus_tag="AC16PP_08205"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR6481647.1"
                     /translation="MDIDFPLVLTWAVLISGAVWLFDLIALKPRREKAAEALTAGGIS
                     EVDAEETLKEPLIVEYARSFFPVLLLVLVLRSFLAEPYQIPSESMVPTLQVGDFILVN
                     KYAYGIRLPVLGTKIVDVGAPERGDVMVFVPPHDSRYFIKRVIGLPGDRIRYEDKALY
                     INGERANYEFIQQFRESVPFSTGYIAIREYSESFGDGRHATYRYAFNEQPKEWVVPEN
                     SYFMMGDNRGKSEDSRVWGFVPEANIVGKAVAIWVHKDPGLHWPTFSRNQWLD"
     gene            2951..3331
                     /locus_tag="AC16PP_08210"
     CDS             2951..3331
                     /locus_tag="AC16PP_08210"
                     /inference="COORDINATES: protein motif:HMM:NF027463.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HIR6481648.1"
                     /translation="MLKRHINMRKQDGAVSLGIVIIIGAVIGAVWLSLRLIPHYIDFE
                     TMKSVMEDLPGPQVHEMDKRAIRDSLEKRFKINNLRTFKVRDVVKIERTSKSTVIDVA
                     YEVREPLMFNIDLVLSFEDQYTYQ"
     gene            3407..4099
                     /gene="rnc"
                     /locus_tag="AC16PP_08215"
     CDS             3407..4099
                     /gene="rnc"
                     /locus_tag="AC16PP_08215"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016978270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR6481649.1"
                     /translation="MRSASEASSLEKLQNALGYQFEDVELLVRALSHRSSGSRHNERL
                     EFLGDAILGYLIAKRLYAVLPAEREDALTLIRSSLVKRDSLARRARRLGLGQHMRLGA
                     GELKSGGHARPSILADALEAVIGAVHEDGGLEAAEALIDRLFDEPVESLDAAAIKDAK
                     TRLQEVLQKEGLGLPDYAIVTTEGSDHQRLFRVSCRVAALELECVAQGSSRRAAEKQA
                     AQAMLEELERRG"
     gene            4092..4985
                     /gene="era"
                     /locus_tag="AC16PP_08220"
     CDS             4092..4985
                     /gene="era"
                     /locus_tag="AC16PP_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR6481650.1"
                     /translation="MAEAVERCGYVALVGRPNVGKSTLLNHLLRQKLAITSRKPQTTR
                     HLLLGVDTNGPHQAIYVDTPGIHDQTDRQINRYMVRAATSVLQDVDVVVMVVDRDVWT
                     EGDEVVLAHVRKARAARFAVINKIDLIRNKNRLLPVIEHLQNLGCFDQVIPVSALRDE
                     GLDALRAEVASSLPQHPHMFPADQLTDQPERFLVAEIIREKLMRRYGDEIPHRTAVVI
                     EGYQEGERVTDIAADIYVERPGQKRIIIGKGGEKLREVGRDARQDIEKLLDRKVMLRL
                     WVKVKPGWTNDRTQLRRMGFD"
     gene            4989..>5335
                     /gene="recO"
                     /locus_tag="AC16PP_08225"
     CDS             4989..>5335
                     /gene="recO"
                     /locus_tag="AC16PP_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR6481651.1"
                     /translation="MSAGRRVSGEPGIVLHVRPYRETSAIVSLLTRHYGRVAGVARGV
                     RGTRRGSPVQPFSSVVTGWSGRGGLLTLTNIESVRQPWLKGEAMNAAFYVVELVTRLV
                     AEGESAPRIFAAL"
BASE COUNT         1072 a         1524 c         1732 g         1007 t
ORIGIN      
        1 tggagcggct tggcatcagc ggtggcgttc gcgtcgtgga ggtcagcggt gccgcggaag
       61 aggccggcct gcggcctggg gacatcatca cccgtatgaa caatcgggag gtcgcagacc
      121 cggcaagcct acgcgagatc gccgacgagc tgccctcggg gaggtccgtg ccggtgctcg
      181 tcatcagggg acaggtgccg acattcctgc ctctgcgggt gccatgaagg cgagagccga
      241 ttggcccggc atctcccgat aagctacact cgcgcgcctt catcgggcac ctggttcgac
      301 agggtttgag cagcgtcgct tccatccgta atttttcgat catcgcgcac atcgaccacg
      361 gcaagtcgac cctggccgat cgcctgatcc agctgtgcgg cggtcttgcc gaccgggaaa
      421 tggaagagca ggtgctcgac tccatggatt tggaacgcga gcgcgggatc accatcaagg
      481 cacacagcgt cacgctgtcc tacaaggctc tggatggcat cgactacaag ctgaacttca
      541 tcgacacgcc cggccacgtg gacttcagct acgaagtttc ccgatcgctt gcagcctgcg
      601 aaggcgcgct gctcgtcgtc gatgccgccc agggggtgga agcgcagtcg gttgccaact
      661 gctacacggc gatcgaccag ggcctggagg tcctgccggt actcaacaag attgacctcc
      721 cccaggcaga gcccgagagg gttgctgcgg agatcgagga catcatcggg ttatctgcgc
      781 acgacgtcct ctgtgtcagc gccaagaccg gctcgggagt gcccgaactg ctcgagcagc
      841 tgatctcgag gatcccgccg ccggagggcg accccgaagc gccgctgcag gccctcatcg
      901 tggattcgtg gttcgacaat tatctgggcg tcgtgtctct ggtgcgcgtg gttcatggcc
      961 gcatcaccaa gggcgaccgg atcgtcgcca agtccgtagg caaggcacac attgtggacg
     1021 aggtgggctg cttcaccccg aaacggtccg gtcgggaatc cctcggggct ggcgaggtgg
     1081 gctacgtggt ggcaggtatc aaagagattc agggggcgcc cgtcggggac accatcgtca
     1141 atgccaagca tcaggaggtt ccccagctgc cggggttcag tcggatcaag cctcaggttt
     1201 acgcggggct tttcccgatc aactccgatg acttcgagag ctttcgcgag gcgctcgaaa
     1261 agctcaagct caacgacgcg gccctgttct acgagcccga atcgtcagac gccctgggct
     1321 tcggctttcg ctgcggcttt ctcgggatgc tccacatgga gatcgtgcag gagcggctgg
     1381 agcgcgagta cgacctcgac ctgatcagct ccgcacctac cgtggtgtac gaggtggttg
     1441 gcgcagacgg tcagctgctg cacatcgaca acccctcgcg gctgccggcc aactaccagg
     1501 aaatacgcga gcccatagcc gaggtgcaca tactggtgcc tcaggattac gtgggcagcg
     1561 tgatgagcct gtgcatcgag cgtcgcggaa cgcagaagaa gatgcattac tcctccggtc
     1621 aggtgcagat gacctgggaa atgccgttga gcgaagtcgt aatggacttt ttcgacaggc
     1681 tcaaatccgt cagcaggggc tttgcctccc tggactacgg tttcgatcgg ttcgaggcga
     1741 gcaacctcgt gcgtctcgac atcctgatca acggcgagcg ggtcgacgcg ctggtgctga
     1801 tcgcacatcg tgaccaggcc cattctcgcg gacggctgct tgcggaaaag atgaaggaac
     1861 tgattccccg gcagctgttc gatgtggccg tccaggcggc cgtggggggg cagatcgtcg
     1921 ccaggcaaac tgtgaaggcg ttacgcaaaa acgtgacggc caagtgctac ggtggtgacg
     1981 taacccgcaa gaaaaagctc ctcgagaagc agaaggccgg taagaggcgc atgaaacagg
     2041 tcggcagggt ggaaataccc caggaagcgt ttcttgccgc tctcaaagtg gaacgctagg
     2101 gcgaaagacg aatggacatt gatttccctc tggttctgac ctgggcggtg ctgatctcag
     2161 gcgcggtatg gctgttcgac ctgatagccc tcaagccgag acgtgagaag gcggctgaag
     2221 ctcttaccgc cggcggaatc tccgaggttg acgccgaaga gacgctcaag gaacccctaa
     2281 tcgtcgagta cgcccggtcg ttctttccgg tgctgttgct ggttctggtg ctgcgcagtt
     2341 ttctcgccga accgtatcag attccgtcag agtccatggt gcccacgctg caggtcggtg
     2401 actttatcct ggtcaacaag tacgcgtacg gtatccggct gccggtgctg ggtaccaaaa
     2461 tcgtcgacgt tggcgctccg gagcggggtg acgtgatggt tttcgtgccg ccccacgact
     2521 cgagatattt catcaagcgg gtgatcggcc tgcccgggga caggatccgt tacgaagaca
     2581 aagcgcttta cataaatggc gagcgagcca attacgagtt catccagcag tttcgcgaat
     2641 cggttccttt ctctaccggt tacatcgcca ttcgcgaata ttccgagagt tttggtgacg
     2701 ggcgtcacgc tacataccga tacgcgttca acgaacagcc gaaagagtgg gttgtgcccg
     2761 agaattccta tttcatgatg ggggacaatc gcggcaagag cgaagacagc cgcgtgtggg
     2821 gcttcgttcc ggaagcgaac atcgtcggca aggcggtggc gatctgggtg cacaaggacc
     2881 ctggactcca ttggccgacg ttcagtcgca accaatggct ggactaaaca aagcatagca
     2941 gaggtgcaag atgctcaaac gtcacatcaa catgcgcaag caggatggcg ccgtgtcgtt
     3001 gggaatcgtc atcatcatcg gcgcggtgat aggggccgtc tggctcagcc tcaggctgat
     3061 tcctcactat atcgacttcg agacgatgaa gtccgtgatg gaggacctgc cgggtccgca
     3121 ggttcatgaa atggacaagc gggcgatccg ggattccttg gagaagcgtt tcaagatcaa
     3181 taacctgcgg acctttaagg tccgtgacgt cgtgaagatc gagcggacca gcaagagtac
     3241 cgtcatcgac gtcgcctacg aggtacgtga gcccctcatg ttcaacattg atctggtgct
     3301 cagcttcgaa gaccagtata cctaccagta gggatcgcaa gcaccaacgc gcttcgccgg
     3361 ggcacacaac cggggggtcg tttctcgcgc tggggggggg tgaatcatgc gttcggcgag
     3421 cgaagcaagc agcctggaaa agctgcagaa tgcgctgggc taccagttcg aagacgtcga
     3481 gctgctcgtg cgtgcgcttt cccatcgaag ctcgggctcg cgacacaatg aacggttgga
     3541 gtttctcggg gatgccatct taggatacct catcgccaag cggctctatg ccgtcttgcc
     3601 agccgaacgg gaggatgcgc ttacgctgat tcgctcgagt ctggtgaagc gcgattcgct
     3661 ggcacgccgg gctcgtcgcc tcggattggg ccagcacatg cggctcggtg ccggagagct
     3721 caagagcggt gggcacgcta gaccgtccat cctcgctgac gctctggaag ccgtcattgg
     3781 ggccgttcac gaggatggcg gcctggaggc tgcggaagcc ctgatcgatc gcctgttcga
     3841 cgagccggtc gagagcctgg acgcggcggc catcaaggat gcaaaaacca ggctgcagga
     3901 ggtgctgcag aaggaaggtt tggggttgcc cgactatgcc atcgtcacga ccgaaggatc
     3961 ggatcaccag aggttgttcc gcgtgagctg cagagtggct gcgctcgagc tggagtgcgt
     4021 agcgcagggg tccagccgac gcgccgccga aaagcaggcg gcccaggcca tgctcgagga
     4081 gctcgaacga cgtggctgag gcagtggagc gttgcggcta cgtcgccctg gtgggtcgac
     4141 ccaacgtggg caagtccacg ctgctgaatc acctgctgcg gcaaaaactg gcaatcacgt
     4201 cccgcaaacc acaaacgacc cggcatctgc tgctgggtgt ggacaccaac gggccgcatc
     4261 aggcgatcta cgtggatacc ccggggattc acgaccagac cgaccggcag atcaatcgct
     4321 acatggtccg cgcagccacc tctgtccttc aggacgtcga tgtcgtcgtg atggtggtgg
     4381 atcgcgatgt ctggacagag ggcgacgagg tcgtgcttgc ccatgtccgc aaggctcggg
     4441 cggctcgctt tgccgtaatc aacaagatcg acctgatccg caacaagaac cgtctgctgc
     4501 ccgtcatcga gcatctgcag aaccttggct gcttcgatca ggtcataccg gtttccgcgt
     4561 tgcgcgatga ggggctcgat gcgctgcggg ccgaggtggc gtcgtccttg ccccagcacc
     4621 cccacatgtt tccagccgac cagctgaccg atcagccgga gcgctttttg gtggcggaga
     4681 tcatccgaga aaaactcatg cgccgctacg gcgatgaaat tcctcatcga acggccgtgg
     4741 tcattgaggg gtaccaggag ggagagcggg ttacggacat cgccgcggac atctatgtcg
     4801 aacgcccggg acagaaaaga ataatcatcg gcaaaggcgg cgagaagctt cgtgaggtgg
     4861 gaagggacgc tcgacaggac atagagaaac tgctcgatcg caaggtcatg ctgcgattgt
     4921 gggtaaaagt gaagcccggt tggacgaacg accggactca gcttcgacgc atgggattcg
     4981 actgagccat gtcggccggt cgtcgcgtgt ccggtgagcc cggcatcgtg cttcacgttc
     5041 gcccttacag agagacgagc gccatcgtct ctctgctgac gcggcattac ggtcgggtgg
     5101 ccggcgtggc gcgtggggtc aggggcacgc ggcgcggatc cccggtgcaa ccgttcagca
     5161 gcgtggtgac cggctggtcc ggacgcggcg gcctgctgac attgacgaac atagaatccg
     5221 tgagacagcc ctggctgaag ggtgaagcca tgaatgcggc gttctacgtc gtcgagctgg
     5281 tgacccgtct cgtcgcggag ggggaaagcg cgccgagaat cttcgccgcg ctgag
//
LOCUS       DBNHKV010000258         3172 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_674197, whole genome shotgun sequence.
ACCESSION   DBNHKV010000258 DBNHKV010000000
VERSION     DBNHKV010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_674197"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..691)
                     /locus_tag="AC16PP_08230"
     CDS             complement(122..691)
                     /locus_tag="AC16PP_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018926809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR6481652.1"
                     /translation="MKMQRHFLISDDLDDLEHLEEELEAQGVSTPQIHVFTLDDTEAE
                     KHVHLHDVQSIMKRDVVNSMIIGAILGAVLSASVLGATALLGWHQTAAGWIPFIFLAI
                     IVLGFCTWEGGFVGIQTLNRHFKQFKATLEQGKHVFYCDLAPDQVPVFQKIVRAHPSA
                     TMAGTGVSTPPWIISWQTRLTRFFGETMP"
     gene            complement(719..1066)
                     /locus_tag="AC16PP_08235"
     CDS             complement(719..1066)
                     /locus_tag="AC16PP_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007977898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HIR6481653.1"
                     /translation="MADTQAATHDAHGEGQQHPIKIYLWIWFLLFLFSAFSYGVDYMQ
                     FQGMLRWSLILIFMFLKAGFIVAIFMHMAWERLALITAILVPPLCLLILIGLMVIEGD
                     YTELTRVIFFQGD"
     gene            complement(1076..1768)
                     /locus_tag="AC16PP_08240"
     CDS             complement(1076..1768)
                     /locus_tag="AC16PP_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007993729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III family protein"
                     /protein_id="HIR6481654.1"
                     /translation="MANNVAAAGVPAFGEGEGWQGIVGDWSADKQAFPVPWGKAMMWI
                     FLLSDTFIFSCFLTSYMKVRISTTIPWPNPSQVFGLDVFGVSVPLLLIAIMTFVLISS
                     SGTMAMAVNFGYRRDKKKTVALMLATAGFGATFVGMQAFEWTKLISEGVRPWGNPWGA
                     AQFGSSFFMITGFHGLHVSAGVIYLVVVARRVANGFYDKKGNYDMVEISGLYWHFVDL
                     VWVFIFAFFYLW"
     gene            complement(1782..2405)
                     /locus_tag="AC16PP_08245"
     CDS             complement(1782..2405)
                     /locus_tag="AC16PP_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR6481655.1"
                     /translation="MKFLKQITEKSWLAPEEDVVADLRSGALVMGTPSAKIGLRVFLT
                     VVLVVFSLFCVAYYIRMELDDWRPLPEPDLLWINTVVILLSSVALQYTRYSVQRSRAG
                     AVNAGLLAAGLLTIGFLVGQYMAWEELRSAGYFAATNPANAFFYLLTAVHGLHLIGGL
                     VVWARTTLKVWNGAEPYQIRLSVELCTTYWHFLALVWLAILWILIST"
     gene            complement(2412..>3172)
                     /locus_tag="AC16PP_08250"
     CDS             complement(2412..>3172)
                     /locus_tag="AC16PP_08250"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019690290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR6481656.1"
                     /translation="PYFGFFFATTTLIIAVPTAIKVYNWVLTLWRGNIRLTTPMLFAI
                     GFVFTFINGGLTGLFLGNVTVDLPLSDTYFVVAHFHMVMGVSPILVVFGAIYHWYPKM
                     TGRMMDETLGKMHFWITFIGTYSIYYPMHYLGFLGVPRRYYAMGNAEFMPESANALNA
                     GISVAALIVGVAQLIFLYNAIVSLRKGKPAGGNPWEATTLEWQTPDTPPIHGNWRDNL
                     PVVHRWAYDYSVPGVDQDYIPQNVPHSETHPAHG"
BASE COUNT          684 a          903 c          996 g          589 t
ORIGIN      
        1 tgatgctcaa agtgccgcgc gccacggagt cagccgagga aaacctgagg acggggtgaa
       61 cggctgatgc agaagaagag ggcgcggggg ggctggccgc cgcgctcctg ctcagggcat
      121 gtcagggcat ggtttcgccg aagaaacggg tcaatctggt ctgccaggag atgatccatg
      181 gaggcgtgga cacgccggtg cctgccatgg ttgcgctggg gtgggcccga acgatcttct
      241 ggaagacggg cacctggtcg ggcgccagat cacagtagaa gacgtgcttg ccttgctcga
      301 gcgtcgcttt gaattgcttg aaatggcgat tgagcgtctg aattccgacg aaaccgccct
      361 cccatgtgca gaagccgagg acgatgatgg cgaggaagat gaatggtatc cagcctgccg
      421 ccgtctggtg ccaaccgagc aacgccgttg cgcccagaac cgatgcgctc agcaccgcac
      481 cgaggatggc gccgatgatc atcgagttca ccacgtcccg cttcatgatg gattggacat
      541 catgcaggtg gacgtgcttt tcggcctctg tgtcgtcgag cgtgaagacg tgaatctgcg
      601 gtgttgaaac gccctgtgcc tcgagctcct cttcgaggtg ctcgagatcg tcgagatcgt
      661 cactgatgag gaagtgccgc tgcatcttca tcttatctcc cctcctaact ggctttactc
      721 agtcaccctg aaagaatatg acgcgggtca gttcggtgta gtcgccttcg atcaccatca
      781 ggccgatgag tatgaggagg caaagtggtg gcacgagaat ggccgtgatc agtgccagcc
      841 tctcccaggc catgtgcatg aaaatggcca cgatgaaccc ggccttcaga aacatgaata
      901 tcaggatgag cgaccagcgc agcattccct gaaactgcat gtagtcgacc ccgtaagaaa
      961 aggcgctgaa gagaaagagc aggaaccaga tccacagata gatcttgatc gggtgctgct
     1021 gtccctcacc gtgcgcgtcg tgagttgccg cttgcgtatc cgccatgttg atttcctacc
     1081 agagatagaa gaacgcgaag atgaataccc agaccaggtc gacgaagtgc cagtacagcc
     1141 cggagatctc gaccatgtcg tagttgccct ttttgtcgta gaagccgttg gcgacccgcc
     1201 tggcaacgac aacaaggtag atcacgccgg cagacacgtg cagtccgtgg aagcccgtga
     1261 tcatgaagaa gctggagccg aactgagctg cgccccaggg attaccccag ggacgaacgc
     1321 cctctgaaat cagcttagtc cactcgaacg cctgcatccc gacgaaggtc gctccgaacc
     1381 cagcagtggc gagcatcagc gccacggtct ttttcttgtc gcgtcggtat ccgaagttga
     1441 cggccatggc catggtgccg ctgctgctga tgagcacgaa ggtcatgatg gcgatgagca
     1501 ggagcgggac gctcacgccg aacacgtcca ggccgaatac ctggctcggg ttcggccacg
     1561 ggatggtcgt ggagattctc accttcatgt agctggtcag aaaacaggag aagatgaagg
     1621 tatcgctgag caggaagatc cacatcatcg ccttgcccca gggcacggga aacgcctgct
     1681 tatccgcgga ccaatccccg acgatgccct gccagccttc gccctctccg aaggcgggaa
     1741 cgccggctgc tgctacgttg tttgccatat tcgtaccatt ctcaggttga aatcaggatc
     1801 caaaggattg ccagccacac cagggccaga aagtgccagt acgtggtgca cagctctacg
     1861 ctcagtcgga tctggtaggg ctccgctccg ttccagacct tcagagtcgt tctggcccag
     1921 accaccaggc cgccgatcag gtgcaggccg tggacggcgg tgagcaggta aaagaacgcg
     1981 ttggccgggt tggttgcggc aaagtaaccg gccgagcgaa gttcttccca ggccatgtac
     2041 tggccgacaa ggaagccgat ggtgagcagc ccagcggcca gcaggcccgc atttacggcg
     2101 cccgcccggc tgcgctgaac ggagtagcgc gtgtactgaa gcgcaacgct gctcagcagg
     2161 atgacgacgg tgttgatcca gagcagatcc ggctccggta acggccgcca gtcgtcgagt
     2221 tccatgcgga tgtagtaggc cacgcagaac agcgagaaca ccaccaggac gacggtcaga
     2281 aagactcgca gcccgatctt ggcggagggc gtacccatga cgagtgcgcc ggaacgcagg
     2341 tcggcgacga cgtcctcctc tggtgccagc cacgactttt ccgttatctg cttgagaaat
     2401 ttcatcgcag atcacccatg ggccggatgc gtttccgagt gcggcacgtt ctgcgggatg
     2461 taatcctgat cgacgccggg gacgctgtag tcgtaggccc atcggtgaac cacgggcagg
     2521 ttgtcacgcc agttcccgtg aatcggcggg gtatccgggg tctgccattc cagcgtcgtt
     2581 gcctcccagg gattgccgcc ggccggcttg cccttgcgga ggctcacgat ggcgttgtac
     2641 aggaagatca gctgagccac gccgacgatg agggcggcca cggagattcc cgcgttgagc
     2701 gcgttcgccg attcgggcat gaactctgcg ttacccatgg cgtagtacct gcgcgggacg
     2761 ccgaggaagc ccaggtagtg catcgggtag tagatggagt acgtgccgat gaacgtgatc
     2821 cagaaatgca tcttgcccag ggtctcgtcc atcattcgcc cggtcatctt cgggtaccag
     2881 tgatagatgg cgccgaacac cacgagtatg ggagagacgc ccattaccat gtggaagtgc
     2941 gcgaccacga agtacgtgtc ggacagcggc aggtccacgg tgacgttgcc caggaacagg
     3001 ccggtcagcc ctccgttgat gaacgtgaag acgaagccga tggcgaacag catgggcgtg
     3061 gtgaggcgaa tgttgccccg ccacaacgtc agcacccagt tgtagacctt gatggccgtg
     3121 gggaccgcga taatcagcgt cgtggtggcg aagaagaacc cgaagtacgg at
//
LOCUS       DBNHKV010000259         1554 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1311887, whole genome shotgun sequence.
ACCESSION   DBNHKV010000259 DBNHKV010000000
VERSION     DBNHKV010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1311887"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="AC16PP_08255"
     CDS             complement(<1..729)
                     /locus_tag="AC16PP_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481657.1"
                     /translation="MWRRHRSWLIGPFLGLWATGSAAQSDFSARVKWFGTATALPEHD
                     LQRNLSGTPAYDQNYDLRLMFQKTISAVTLQVEHSTTLVTGDSLNFPTAPGTVLEQSP
                     EGDERRLMDLTWEIEDGDRHRSFHRLDRLALKLRRDDWAVTVGREAVSWGAGLVFRPM
                     DLFNPFAPTAVDRDYKAGDDLLLIERLFSDGSDLQVLAVGRRDETGDFTGQAGSLAGK
                     WRGFIGACEVELMAAKHYRDQVYGI"
     gene            complement(713..1414)
                     /locus_tag="AC16PP_08260"
     CDS             complement(713..1414)
                     /locus_tag="AC16PP_08260"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6481658.1"
                     /translation="MAERVVRCIGVSRTYQDDSVPVHALRDVDFEVGKGEFVTLAGPS
                     GSGKSTLLNMIGGLDRPSSGCVEVDGVDLGSLSESRLSDLRLRKIGFVFQSYNLIPVL
                     SARENVEFIMQLQGVSAAERAARARAALEALGIGELEKRRPGELSGGQQQRVAVARAI
                     VTEPVLLLADEPSANLDSATTRELLEYLRRLNQDRGVTIVAATHDPMVMGYAQRQVGL
                     RDGAIVKDSADVAPA"
BASE COUNT          286 a          544 c          479 g          245 t
ORIGIN      
        1 aatcccgtat acctgatcgc ggtagtgctt ggcggccatc agctccacct cgcaggcccc
       61 gataaacccg cgccatttgc ccgcgaggct ccccgcctgg cccgtgaaat cacccgtctc
      121 gtcacgccgt cccaccgcga gcacctgcag atcgctgcca tcggagaaga ggcgctcgat
      181 cagcaggaga tcgtcgcccg ccttgtaatc ccggtccacc gcggtgggcg cgaacgggtt
      241 gaacaggtcc atgggccgga ataccaggcc ggcgccccag ctgacggcct cgcggccaac
      301 ggtgacggcc cagtcatccc tgcgcagctt gagggccagc cggtcgaggc gatggaacga
      361 gcggtgccgg tcgccgtctt cgatctccca ggtcaggtcc atcagccggc gctcgtctcc
      421 ctcgggggac tgttcgagaa ccgtgccggg tgccgtgggg aagttcagcg aatctcccgt
      481 gaccagcgtg gtcgagtgct ccacctgcag cgttaccgcc gagatcgtct tctgaaacat
      541 caggcgcagg tcgtaattct ggtcgtaggc gggggtgccg ctgaggttgc gctgaagatc
      601 gtgctccggc agggcggtag ccgtgccgaa ccatttgacc cgagcggaaa agtccgactg
      661 cgccgcgctg cccgtcgccc acagaccgag aaacggcccg atcagccagc ttctatgccg
      721 gcgccacatc ggcgctgtcc ttcacgatcg cgccgtcgcg cagcccgacc tgacgctgag
      781 cgtagcccat gaccatggga tcgtgggtgg cggcaacgat ggtcacgccg cggtcctggt
      841 tgaggcgacg cagatactcg agcagctccc tggtggtggc cgaatcgaga ttggcgctcg
      901 gctcgtcggc caacagcaga accggctcag tgacgatggc cctcgcaacc gccacccgct
      961 gctgctgacc tccggacagc tcccccggac gtcgcttctc cagctcgccg atgcccaggg
     1021 cctcgagcgc ggcgcgagcc cgagcggcac gctccgcggc cgacacgccc tgcagctgca
     1081 tgatgaactc cacgttctcc cttgcggaca gaacgggaat gaggttatag gactggaaaa
     1141 cgaagccgat cttgcgcagc cgcagatccg acagccgact ctcgctgagc gaaccgaggt
     1201 caacgccatc cacctcgacg catccggagc tggggcggtc cagcccgccg atcatgttga
     1261 gcaacgtgga ctttcccgat ccggacggtc ccgccagcgt cacgaactcg cccttgccga
     1321 cctcgaagtc cacatcccga agagcatgca cgggcaccga atcgtcctgg taggtccggg
     1381 agaccccaat acagcgcact acccgttcgg ccatttcttc atcatctccg caaagcatcc
     1441 agggggccga tctgcacggc acgccatgcc gggtagagac tcgcaatcag gccaaagaca
     1501 atgctcatgg ccgcgaccag cacgacgtcc gcgaggagaa gcctcggaac gagc
//
LOCUS       DBNHKV010000260         7412 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_35330, whole genome shotgun sequence.
ACCESSION   DBNHKV010000260 DBNHKV010000000
VERSION     DBNHKV010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7412)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_35330"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="AC16PP_08265"
     CDS             complement(<1..510)
                     /locus_tag="AC16PP_08265"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR6481659.1"
                     /translation="MKAALMENGRIWVDTVPDPEPRLGEVLVKSAGCGICGSDLHAYR
                     HTEEFVATSRESGGAFRLTTMNPVVLGHEFCAEVVDYGPGTQRRLPVGTLVCAIPALP
                     RGGGFEPIGYSEDAPGGFAEYFLLNERLMMPVPPGLSWELAALTEPMAVGHHAINKAR
                     LDGEAVLVIG"
     gene            complement(532..1824)
                     /locus_tag="AC16PP_08270"
     CDS             complement(532..1824)
                     /locus_tag="AC16PP_08270"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6481660.1"
                     /translation="MASNPQSGDVSDIQLFDVETLKDPWDAYARLRDHAPVFFVPGFD
                     IHVVTRYDLVREVIKDTETYSSKFDLFLNRSRELMFASAPPEVQAELIRLNDEMIQIP
                     PTMLTLDEPEHTKYRSLVSQLFTVSQIRKAEDAVQAVIDETLETFVDRRSVDFMDAFA
                     FPVPLRIIADRLGIPEDDRAFFDDAATAAAAGLRLSPITPEEMVRRAQLGVDLQKLLV
                     RIIEERRTDPLEDMISILANSKLEAEDRYLTHGECLSILNQFLVAGHETTTSTFGWGM
                     LLLCRNPEAQRALRADPSLTRTFVEEALRLEAPVQGLPRLVTKDTELGGVELEAGKLI
                     MIRYGAANRDGRQFENPDELDLHRKKAGMQLAFGSGVHHCIGAPLARQELNLGFPTLL
                     RHMSEIRLSADHPPPEAEASFILRNLPELHIDYDLIEP"
     gene            1966..3519
                     /locus_tag="AC16PP_08275"
     CDS             1966..3519
                     /locus_tag="AC16PP_08275"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6481661.1"
                     /translation="MTDPLTELARSQPDKPALIDDRPDGTLLKWTYSELEERTNALAR
                     VFQNAGVEAGNRLIWCGPNSAWVVAAGIAARKIGAVAVPLNYRLTAEEAAYVVDNSDA
                     VTVFADAEYADLFAEIRDDIPQVREVLIFDGPAGPGQTSAEERLAQEGTEELAVKVDP
                     TTSGTMIYTSGTTGRPKGAVRKDTGDPALIAALMELMQLRGDDVYLTTGPLYHSGPSG
                     FMAFAFAFGNTIVLQRKFDAEDWLRLIDKYGVSFTFSAPTPVRLATQLPPETIKRYDL
                     SSVRITIANAAPWSFALKKAYLEHFPADSLFEVYGSTELGVNTIMLPADQLRKPGSCG
                     QAAPGVEIILIDEEGREVTEPNVPGEVFIRSPSVFSTYHKAEDKYLEDRRGDMQTVGD
                     IAYRDDEGFYYICDRKKDMIISGGVNVYPAEIEAALEHHPDILDVAVFGIPSEEWGES
                     VHAVVVKRSGSGLNEEAIQAFAREHLAGYKVPRSIEYRDAIPRTGTGKILKRELRAPW
                     WEGHNSSVS"
     gene            complement(3619..4518)
                     /locus_tag="AC16PP_08280"
     CDS             complement(3619..4518)
                     /locus_tag="AC16PP_08280"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6481662.1"
                     /translation="MTEAFDVVGDWQVARLAGALGAEVRGIDLRQVTSEEVDRIRSLL
                     LDHLVLFFPDQNISVDDHVSFGRYFGELEGHPHLDNPFTEHPELFELAASRGGVADEW
                     HTDITFRDEPALMSILHMVRCPTVGGDTMWSSLYAAFEELSEPMRDLCEGLTALHDAL
                     PHGRPEQTAIHPVVRVHPETGRKALYVNEHFTRRIVEMNARESDMLLGYLTKWVQNPR
                     FTVRYRWAEGTIAMWDNRCTQHFVLNDFEGERVIQRVTVMGDRPEGSGARWAPAVRTG
                     RHSATSRYDAQLVRHLRSREDQA"
     gene            complement(4603..5811)
                     /locus_tag="AC16PP_08285"
     CDS             complement(4603..5811)
                     /locus_tag="AC16PP_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6481663.1"
                     /translation="MVPTETEIREFDLRNPPAGFVEDPFFWYEALRAGGPVRRMPDGS
                     MLVTGYDACSDIYRNEAFISDKRNLFRPKFGDSPLYEHHTTSLVFNDPPYHTRVRRTI
                     VEALKPRSIQPTLDMLARRIQELLASMGEAGEADLIEAYASRIPVEVICSLLTVPTQD
                     RDDLRRWSLAILGALEPAISESEAEQGNRAVTEFLGYLRTLIAERRRRPLAEDSVLSS
                     LLAQQDRGEVSELELLHNCIFLLNAGHETTTNLIGNGVHMLLTHDSERDLLVAHPSRI
                     KSAVEEILRYQSPNQLGNREVTGNVTMRGQRFAPGDQVTLCIAAANRDPRKFPDAHRF
                     LIDRAPNPHLAFAAGPHTCAGMALARIEGKLALSAFFQAFPEATLQARPRYRDRIRFR
                     GLEALHVRLQ"
     gene            complement(5857..7152)
                     /gene="hmgA"
                     /locus_tag="AC16PP_08290"
     CDS             complement(5857..7152)
                     /gene="hmgA"
                     /locus_tag="AC16PP_08290"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003152943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HIR6481664.1"
                     /translation="MTGVLEYLAGFGNEHQTEALPGALPVGRFSPQQPALGLYAEQLS
                     STAFTVPRALNRRTWFYRIRPSVMHGDFRPMQTGLLRTSPVDQGAPLPDQMRWDPLEV
                     PEEPADFLDGLITMAVNGNAATQAGVGVHVYLATTSMGRRYLYDADGELLIVPQLGSL
                     LVRTECGALDVSPGEICVVPRGLKFAVDVPNPPVRGYVCENYGWPFLLPERGPVGANG
                     YANDRDFLYPTARFEDVDGHHELVCKLSGNLFVAEMDHSPLDVVAWTGNSAPYKYDLG
                     RFNVMGSVSFDHPDPSIYTVLTSPSETAGMANADFVIFPPRWIVAEDTFRPPWYHRNV
                     MSEFMGLVRGLYDAKETGFVPGGCSLHNSMAAHGPDVEAFEKASSADLKPQKLADTLA
                     FMFESRYPYTATAFAMESPALQSDYRACWAALPRMFEPQ"
     gene            complement(7152..>7412)
                     /locus_tag="AC16PP_08295"
     CDS             complement(7152..>7412)
                     /locus_tag="AC16PP_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HIR6481665.1"
                     /translation="MERLSLQRGDRVTVRSAHGVMHGALVEPFDLPAGNLMAYFPEAN
                     VLTGTSVDPRSKTPSFKATPVCIERSLTTPLRVEPSRGENAP"
BASE COUNT         1405 a         2379 c         2364 g         1264 t
ORIGIN      
        1 gccgatgacg agaaccgctt cgccatcgag ccttgctttg ttgatggcgt ggtggccgac
       61 ggccatgggc tccgtcagcg cggcaagctc ccaggatagc ccagggggta cgggcatcat
      121 caatcgctcg ttgagcagaa agtattcggc gaaccccccc ggcgcgtcct ccgagtaacc
      181 gatgggctcg aaaccgccgc cccgcggaag cgcgggaatg gcacagacca aggtgccaac
      241 gggaaggcgg cgctgcgtcc cgggtccgta gtcaacgacc tcagcgcaga actcgtggcc
      301 caggaccacc ggattcatgg tggtcaggcg gaaagcgccc ccggactcgc ggctggtcgc
      361 cacgaactcc tccgtgtgac ggtaggcatg gaggtcagat ccgcatatcc cgcaacccgc
      421 ggacttgacc agcacctctc cgagtcgagg ctcgggatcg gggacggtgt ctacccagat
      481 ccgaccattt tccatgagcg ccgctttcat gatgatccgg cgtctcggcg atcagggctc
      541 gatcaagtcg tagtcgatgt gcagctccgg caggttgcgg agaatgaagc tggcctccgc
      601 ctccgggggc ggatgatcgg ccgatagacg aatctcgctc atgtgccgta gcagggtcgg
      661 aaaacccaga ttcagctctt gccgggccag cggggcgccg atgcagtgat gaaccccgga
      721 accgaacgcc agctgcatgc ccgctttctt gcggtgcagg tcgagctcgt ccggattctc
      781 gaactgacga ccgtcgcgat tggccgcacc gtagcggatc atgatcagct tgcctgcctc
      841 cagctcgacg ccgccgagtt cggtatcctt cgtcaccagg cgcgggagcc cctggacggg
      901 tgcttccagc cgcagggcct cctccacgaa ggtccgcgtg agtgacggat cggcgcgcag
      961 cgcccgctgg gcctcggggt tgcggcacag cagcagcatt ccccagccga acgtgctcgt
     1021 cgtggtttcg tgtcccgcca cgagaaactg gttgaggatg ctcagacact cgccatgagt
     1081 gaggtaacga tcctcggcct ccagcttgct gttcgcgagg atgctgatca tgtcctcgag
     1141 agggtcggtc cgtcgctctt cgattatccg gaccagcagc ttctgcagat ccacccccag
     1201 ctgggcccgg cggaccattt cttcgggcgt gatggggctg agccggagac cggccgccgc
     1261 ggcggtggcc gcgtcgtcga agaacgctcg gtcgtcctcg ggaataccca gccgatcggc
     1321 gatgatcctc agcggcacgg gaaacgcaaa cgcgtccatg aagtccaccg agcggcggtc
     1381 aacgaacgtt tccagcgtct cgtcgatgac ggcctgaacg gcgtcctcgg ccttgcggat
     1441 ctgactgacg gtgaacagct gcgataccag cgaccgatac ttcgtatgct cgggctcgtc
     1501 gagcgtgagc atcgtgggtg ggatctggat catctcgtcg ttgagacgga tgagctccgc
     1561 ctgcacctcg ggcggtgccg atgcaaacat cagctcccgg gaacggttga ggaacaagtc
     1621 gaacttgctc gagtaggttt ccgtgtcctt gatgacctcg cgcacgaggt cgtatcgcgt
     1681 cacaacatgg atatcaaaac caggtacgaa gaaaacgggg gcgtgatcgc gcaggcgggc
     1741 ataggcatcc caggggtcct tgagggtttc tacgtcgaac agctggatgt cagagacatc
     1801 cccagattgc ggattgctgg ccataagcac actccccgaa gttcgttcgt tcccggagaa
     1861 ttctacgcct gctgcccgtc ggttacgaat ggctgcggat gcacgaccac gaacgcccgg
     1921 ctaccatcag cccgggaaac tcattcgggg cacgaggaga gcgagatgac ggatccgttg
     1981 accgagctgg cgcgcagcca accggataag ccagcgctga tcgacgaccg gcccgacgga
     2041 accctgctga agtggacgta ctcagagctg gaagagcgca cgaacgccct cgcacgggtc
     2101 tttcagaacg ccggcgtgga agccggcaac cggttgatct ggtgcgggcc caattctgca
     2161 tgggtggtcg cggccggcat cgccgcgcgc aagatcggcg cagtcgccgt gccgctgaac
     2221 taccggctga ccgcagagga agccgcctac gtggtggata actccgacgc cgtgacggtt
     2281 ttcgcagatg ccgagtacgc cgacctgttc gccgagattc gggacgacat cccccaggtt
     2341 cgggaggtcc tgatattcga cggtccggct ggaccgggac agacgtcggc cgaggagcgc
     2401 ctggctcaag agggcaccga ggaactcgca gtgaaagtcg acccgacgac ctccggcacc
     2461 atgatctaca cctccgggac caccggtcgt ccgaaaggcg cggtgcgcaa ggacaccggc
     2521 gatccggccc tgattgccgc gctgatggaa ctgatgcagc tcaggggcga tgacgtctac
     2581 ctgaccaccg ggccgctcta tcactccgga cccagcggct tcatggcgtt tgcgttcgcc
     2641 ttcggcaaca ccatcgtcct gcagcgcaaa ttcgacgccg aggactggct gcgactcatc
     2701 gacaagtatg gggtctcctt caccttcagc gcgccgacgc ccgtaaggct agccacgcag
     2761 ttgccgcccg agaccatcaa gcgctatgac ctctcgagcg tgcgcatcac catcgccaac
     2821 gcggctccgt ggagcttcgc gctgaaaaag gcctacctcg agcactttcc ggccgacagc
     2881 ctgttcgagg tttacgggtc caccgagctt ggcgtgaaca ccatcatgct gcccgcggac
     2941 cagctcagaa agccggggtc ctgcggtcag gccgcgcccg gtgtggagat cattctcatc
     3001 gacgaggaag gccgggaggt cacggaacca aacgtccccg gcgaggtgtt catccgctcg
     3061 ccgagcgtgt tctccaccta tcacaaggca gaggacaagt atctggaaga ccgccgcggc
     3121 gacatgcaga cggtgggcga catcgcctat cgcgacgacg aaggcttcta ctacatctgt
     3181 gatcgcaaga aggacatgat catctccggc ggggtcaacg tctatccggc ggagatcgaa
     3241 gccgcgctgg agcatcaccc cgacatactc gacgtcgccg tgttcggaat ccccagcgag
     3301 gagtggggag agagcgtgca cgccgtcgtc gtgaaacgca gcggctctgg attgaacgag
     3361 gaggccattc aggcattcgc ccgggagcac ctggcgggct acaaggttcc gcgctcgatc
     3421 gaatatcgcg acgcgatccc tcgcacggga accggcaaga tcctcaagcg ggagctgcgc
     3481 gcgccctggt gggaagggca caacagcagc gtttcgtaga accgcagccc ggtgacgtga
     3541 agaccatcgc gcggcgggtg ccccaaggcg caagaggcga acgaccctgc accggaagcg
     3601 gcccaggcgc ggccggcgct aagcctgatc ctcccggctg cgcagatggc gaacgagctg
     3661 ggcgtcgtaa cggctggtgg cagagtgccg gcccgtgcgg accgccggag cccaacgcgc
     3721 cccgctgcct tccggccggt ctcccatcac ggtcacccgc tgaatgacgc gttccccttc
     3781 gaaatcgttc agaacgaagt gctgggtgca gcggttgtcc cacatggcga tggttccctc
     3841 cgcccagcgg tagcgcacgg tgaaacgggg gttctgcacc cacttcgtga gatagcccag
     3901 cagcatgtca ctttccctgg cgttcatctc cacgattcgc cgggtgaaat gctcgttcac
     3961 gtaaagcgcc ttgcgcccgg tttccggatg cacgcggacc accggatgaa tggccgtctg
     4021 ctccgggcgg ccatggggca gcgcgtcatg cagggccgtg aggccctcgc agagatcgcg
     4081 catgggttcc gagagttcct cgaacgccgc gtaaaggctc gaccacatgg tgtccccgcc
     4141 aacggttggg catcgcacca tgtgcagaat cgacataagc gcgggctcgt cccgaaaagt
     4201 gatgtccgtg tgccactcgt cggcgacgcc gccgcggctc gctgccagct cgaagagctc
     4261 ggggtgctcg gtgaacgggt tatccagatg gggatggccc tcgagctcac cgaaataccg
     4321 tccgaacgag acgtggtcgt ctacgctgat gttctgatcg ggaaagaaca ggaccaggtg
     4381 gtcgagcaac aggctccgga tccggtccac ctcctccgac gtcacctgtc taagatcgat
     4441 tccgcgaacc tccgcgccca gggcgccagc cagccgtgcc acctgccaat cgccgacaac
     4501 atcgaaagct tcagtcatga tcgtccatca gattccgcgt cacgtaggca gagtatataa
     4561 cgcttccggg cctgcaagcc gccaaggccc cttgtgccgc gttcactgca agcggacgtg
     4621 gagcgcttcc aggccgcgga agcggatccg atcacgatac cgtggccgcg cctgaagggt
     4681 cgcttcggga aacgcctgga aaaatgcgct gagcgcaagc tttccctcga ttcgcgcaag
     4741 ggccatgccc gcgcaggtat ggggacccgc ggcaaaggcc aggtggggat tcggcgcgcg
     4801 atcgatcagg aaacgatggg catccggaaa tttgcgcggg tcgcgattcg ccgctgcgat
     4861 gcacagggtc acctgatcac caggcgcaaa gcgttgccca cgcatcgtta cgttccccgt
     4921 cacctcacgg ttcccgagct ggttggggct ttgatagcgc aggatctcct ccaccgccga
     4981 cttgattcgt gaaggatgcg caaccagcag gtccctttcg gagtcgtggg tcagcagcat
     5041 gtgaacgcca ttgccgatca gattggtcgt ggtctcgtgt ccggcgttca gaagaaagat
     5101 gcagttgtgc agcagttcga gctcgctcac ctcgccccga tcctgctgag ccaggagcga
     5161 ggacagaacg ctgtcctcgg cgagggggcg gcgccgacgc tcggcgatca gcgtcctgag
     5221 ataccccaga aattccgtta ccgcgcggtt gccttgctct gcctcggatt cggagatagc
     5281 gggctccagc gcacccagaa tggccagcga ccagcgccgc aggtcgtccc tgtcctgcgt
     5341 cggcaccgtg agcaggctgc agatgacttc caccggaatc cgggacgcgt acgcttcaat
     5401 gaggtccgcc tctcccgcct cccccatcga tgcgagcagt tcctgaatgc gccgggcgag
     5461 catgtctagg gtcggctgga tcgagcgcgg cttcaacgcc tccacgatgg tccgccgaac
     5521 ccgcgtgtgg tacggagggt cgttgaagac cagactcgtc gtgtgatgct cgtacagcgg
     5581 cgagtctccg aacttcggcc ggaagaggtt tcgtttgtcc gaaatgaacg cctcgttccg
     5641 atagatgtcc gagcacgcgt catatccggt aaccagcatc gacccgtcgg gcatccggcg
     5701 gacgggaccg ccggcgcgaa gcgcttcgta ccagaaaaag ggatcctcta cgaacccggc
     5761 cggcgggttg cgcaggtcga attcacgaat ttccgtctcc gtcggcacca tcggcatctc
     5821 accccgcccg tgggccggcc gccatccccg tgcgcatcac tgcggctcga acatccgagg
     5881 cagcgccgcc cagcaggctc ggtaatcgct ctgcagggcc gggctttcca tggcgaacgc
     5941 ggtagccgtg tagggatagc gggactcgaa catgaacgcc agcgtgtcgg ctaacttctg
     6001 cggcttcaga tccgccgagc tcgccttctc gaaggcctcc acatcgggac cgtgagccgc
     6061 catgctgttg tgcaggctgc acccgccggg cacgaagccg gtctctttgg cgtcgtacaa
     6121 accccgcacc agccccatga actcgctcat gacgttgcgg tggtaccagg gcggacgaaa
     6181 ggtgtcttcg gcgacgatcc agcgcggcgg aaagatcacg aagtccgcgt tcgccatgcc
     6241 cgcggtctcg gagggcgagg tgagcaccgt gtagatggac ggatcgggat gatcgaagct
     6301 caccgagccc atgacgttga atcggcccag atcgtacttg tacggcgccg agttgcctgt
     6361 ccaggccacg acgtccagag gcgaatgatc catctccgcg acgaacagat ttcccgataa
     6421 cttgcacacg agctcgtggt gaccgtccac gtcctcaaac cgtgccgtgg ggtaaagaaa
     6481 gtcccggtcg ttggcatatc cgttcgcgcc caccggaccg cgctcaggga gcagaaacgg
     6541 ccagccgtag ttctcgcaga cgtagccgcg aacgggtggg ttcggcacat cgacggcgaa
     6601 cttcaagccg cgcgggacga cgcagatctc gcccggggag acatccaggg caccgcactc
     6661 cgtgcgaacc aaaagcgatc ccagctgggg aacgatcagc aactcaccgt cggcatcgta
     6721 gaggtagcga cgacccatgg acgtagtcgc aagatagacg tgcacgccca caccggcctg
     6781 agtggccgcg tttccgttca ccgccatggt gatcagaccg tccaggaagt cggccggctc
     6841 ctccggcacc tctagcggat cccagcgcat ctgatcgggg agcggcgcgc cctggtcaac
     6901 gggcgaagtg cgcagaagcc cggtttgcat cgggcggaag tcgccgtgca ttaccgatgg
     6961 ccggattcga tagaaccacg tgcggcgatt gagggcccgg ggcaccgtga acgccgtcga
     7021 gctcagctgc tccgcgtaca ggccgagcgc cggctgctgg gggctgaagc ggccgacggg
     7081 gagcgcaccg ggaagcgcct cggtctgatg ctcgttgccg aaccccgcta gatactcgag
     7141 gacgcccgtc actagggggc gttctcgccg cgactgggct ccacccgcag cggcgtagtc
     7201 aaagaccgtt ctatgcagac cggtgtggcc ttgaacgacg gggtcttgct gcgaggatcc
     7261 accgacgtcc cggtcagcac gtttgcttcc ggaaagtagg ccatgagatt tcccgcaggc
     7321 agatcgaaag gttccaccag cgcgccgtgc atgacaccgt gagcagacct cacggtcact
     7381 cggtcgcctc gttgcaggga cagcctctcc at
//
LOCUS       DBNHKV010000261         3371 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1029348, whole genome shotgun sequence.
ACCESSION   DBNHKV010000261 DBNHKV010000000
VERSION     DBNHKV010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1029348"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="AC16PP_08300"
     CDS             <1..936
                     /locus_tag="AC16PP_08300"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6481666.1"
                     /translation="YFLDETDCFWAPLPMFHMASILPMICCMDAGAALQSMTHVEAGG
                     ALAMMERDATTVAFPSFPTVTNELINHPEFTTRDLSRIRRINNVAPPDVLRKFQDAFP
                     QAVQTGAYGLTEVGGVIAFNHPDESLEQRLTTCGRPFPGVEVKVVDPDTGAELGPGER
                     GEILVRGYAVFDGYYKAPEKNAEAFADGWFRTGDLCALGADGSIEFHGRIKDMLKVGG
                     ENVAALEIESYLATHPAVKLAQVIGVPDERLLEVPAAFIELNPNAFATAQELIDYCKG
                     KIASFKVPRHIRFVDEWPMSSTKVQKFRLLEQFEA"
     gene            1031..2449
                     /locus_tag="AC16PP_08305"
     CDS             1031..2449
                     /locus_tag="AC16PP_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6481667.1"
                     /translation="MAEQAAHQELTPQEEIRGLIERSRAAQAQIENYTQEQVDELIRA
                     MVWCVAKPGVAEEIAQFTVDETQLGNYDGKYLKIHRKTRATLMDIINDKSVGILEEDK
                     ERNIVKIAKPMGVIGALAPSTNPEATPVIKAIHAVKGRNSIIVAPHPRAKLTNKMICD
                     KMREAIAACGAPADLVIAIESPSLELTNELMRQCDRVLATGGGAMVHAAYSSGTPALG
                     VGVGNAVITVDETADLEDAAEKIRLSKTLDLAASCSADNSVILVDAIYDEMMNKLEHQ
                     GGYLCNAEEKAKLQSTLWDDEGHLNTAIVAQPAEKIAAMAGIELPEGRSFYIVPEDGW
                     GPEHPFSGEKLSVIMALYRAKDIDHAIELTNNIQSYQGQGHSCGIYSTSDDNIMKLAN
                     ATKTSRVMVNQPQAASNSGNLWNGMRQTFSLGCGSWGGNGTNHNISWRDLINETWVSK
                     PLAKTKELPSDEELFGDVMGRI"
     gene            2764..>3371
                     /locus_tag="AC16PP_08310"
     CDS             2764..>3371
                     /locus_tag="AC16PP_08310"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6481668.1"
                     /translation="MFNATRRSTGCRALTLTTLAVMLSLLASGASAETTEQPSDDVLL
                     EEMVVTVERRSQSLQDYAGTAAAFTGEELKSLGVQNIQDLDRFLPGFSVANNGGNIEV
                     YIRGIGSSNNTELGDPAAATHLNGVYVPRPSGFGSAFFDIERVEVNIGPQGTLRGRNA
                     TAGSVDIIPWKPGLGITDASVEASIGNYGEWSVEGMVNVPVG"
BASE COUNT          693 a         1092 c         1051 g          535 t
ORIGIN      
        1 tacttcctcg acgagaccga ttgcttctgg gctcccctgc cgatgttcca catggcctcc
       61 atcctgccca tgatctgctg catggatgcc ggtgccgccc tgcagtccat gacccacgtc
      121 gaggccggcg gggccctggc gatgatggag cgcgacgcaa ccaccgttgc cttcccgtcc
      181 tttccgacgg tcaccaacga gctgatcaac catcccgagt tcaccacccg ggacttgagc
      241 cgtatccgcc ggatcaacaa cgtcgcgccg ccggacgtgc tgcggaaatt tcaggacgcg
      301 tttcctcaag ccgtgcagac gggcgcctac gggctcaccg aggtgggcgg cgtgatcgcc
      361 ttcaaccacc cggacgagag cctggagcag cgtctcacca cctgcggccg gccatttccc
      421 ggcgtcgagg tcaaagtggt ggatccggat accggcgccg agctgggacc gggagaacgc
      481 ggcgagatcc tggtgcgcgg ctacgccgtt ttcgacggct actacaaggc gccggagaag
      541 aacgcggagg cttttgccga cggctggttc cggaccggcg atctgtgcgc gctgggcgcc
      601 gacggctcca tcgagtttca cggacgcatc aaggacatgc tcaaggtggg gggagagaac
      661 gtggcggcgc tggagatcga gtcttacctg gcaacgcatc cggcagtgaa gctcgcccag
      721 gtgatcggcg tgcccgacga gcggctgctg gaagtgcccg ccgctttcat cgagctgaac
      781 cccaatgcct tcgccactgc gcaggagctg atcgactact gtaaagggaa gatcgcgagc
      841 ttcaaggttc cgcgccacat ccgcttcgtg gacgagtggc ccatgtcgtc caccaaggta
      901 cagaagttcc ggctcctgga gcagttcgaa gcttgagaaa ggcttttgtc aggccagata
      961 cggttgtgta cagtatccgg ttcggcggtc ccgcatccgg gccaccatcg ttgtcaaggg
     1021 agattccgct atggctgagc aggccgccca tcaagagttg accccgcaag aagagattcg
     1081 cgggctcatc gagcgctcgc gcgcagcgca ggcgcagatc gaaaactaca cccaggagca
     1141 ggtggacgag ctcatccggg ccatggtctg gtgcgtagcg aagccgggcg tggccgagga
     1201 gatcgcccag ttcaccgtgg acgagaccca gctcggtaac tacgacggca agtacctgaa
     1261 gatccaccga aagacccgcg cgacgctgat ggacatcatc aacgacaagt cggtcggcat
     1321 cctcgaggag gacaaggaac ggaacatcgt caagatcgcc aagcccatgg gcgtgatcgg
     1381 tgccctggcg ccatccacca acccggaagc cacgccggtg atcaaggcca ttcacgccgt
     1441 gaaggggcgc aactccatca tcgtcgcgcc tcatccgcgg gcaaagctca ccaacaagat
     1501 gatctgcgac aagatgcgcg aggccatcgc cgcctgcggc gcgccggcgg atctggtcat
     1561 cgccattgaa tcccccagcc tggagcttac caacgagctg atgcggcagt gcgaccgggt
     1621 gctagccacg ggcggcggcg ccatggtcca cgcggcctac tcgagcggca cgcccgccct
     1681 gggtgtcggg gtgggcaacg cggtgatcac ggtcgacgag acggccgatt tggaagatgc
     1741 ggcggaaaag atccgcctgt ccaagacctt agacctcgct gcctcctgct ccgccgacaa
     1801 ctcggtgatc ctggtggacg ccatttacga cgagatgatg aacaagctgg agcaccaggg
     1861 cggctatctg tgcaacgccg aggaaaaggc caagctgcag agcacgctgt gggacgacga
     1921 ggggcacctc aacacggcca tcgtcgccca gccggcggag aagattgccg ccatggcggg
     1981 gatcgagctg cccgagggca gaagcttcta catcgtcccg gaggacggct ggggcccgga
     2041 gcatcccttc tccggcgaga agctgtcggt gatcatggcg ctctacagag caaaggacat
     2101 cgatcacgcc atcgagctga ccaacaacat ccagtcgtac cagggccagg ggcattcctg
     2161 cgggatctac tccaccagcg acgacaacat catgaagctg gccaacgcca ccaagacctc
     2221 acgggtcatg gtcaaccagc ctcaggcagc gtccaacagc ggcaacctgt ggaacggcat
     2281 gcgtcagacg ttctccctgg gctgcgggtc ctggggcggc aacggcacca accacaacat
     2341 ctcctggcgg gatctcatca acgagacctg ggtgtccaag cccctggcga agaccaagga
     2401 gctgccgtcg gacgaggaac tgttcggaga cgtgatgggc cggatttagc ggcccgggcc
     2461 gaccggggcc ccgccatcac caatcaaccg ggcgtcctgt ggacgcccgt tttttttgcg
     2521 gacctctcca gcaaaacatc aaacgcaagt gggaaggccg ccgtcgtccc cacgagcggc
     2581 cccgggcggg gacccgatgc gcgcccgcac aattccggag acaaatttga cagcgctgtc
     2641 ataactgtgt atatcctgtt ccaccgcgat tatgacagcg atgtcatcgc gcactccaca
     2701 agggacgcca atccgatgcc ccaggagttg acccggaata aacgcgaggg ggatcctcaa
     2761 atcatgttca acgcaacacg acgcagcacc ggctgcagag ccctgacatt gacgaccctg
     2821 gcggtcatgc tgagcttgct ggcttcagga gcctcagcgg aaaccaccga gcagccatcc
     2881 gacgacgtgc tcctcgagga gatggtcgtc accgtggaac ggcggtccca gagcctgcag
     2941 gattacgcag gcaccgcggc agcattcacc ggggaggagc tgaagtctct gggcgttcaa
     3001 aacatccagg acctcgaccg cttcctgccg ggattctcgg tggccaacaa cgggggcaac
     3061 atcgaggtct acatccgcgg catcggctcc agcaacaaca ccgagctcgg tgacccggcg
     3121 gctgccacgc acctcaacgg cgtctacgtc ccgcgtccga gcgggttcgg ctccgccttc
     3181 ttcgacatcg agcgagtcga ggtcaacatc gggccccagg gcacgctcag aggccgcaac
     3241 gccaccgccg gctccgtgga catcattccc tggaagcccg gtctgggcat caccgacgca
     3301 tccgtcgagg catccatcgg caactacggt gagtggtcgg tcgaaggcat ggtgaacgta
     3361 cccgtcggcg a
//
LOCUS       DBNHKV010000262         3018 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_603155, whole genome shotgun sequence.
ACCESSION   DBNHKV010000262 DBNHKV010000000
VERSION     DBNHKV010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_603155"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..942)
                     /locus_tag="AC16PP_08315"
     CDS             complement(70..942)
                     /locus_tag="AC16PP_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010445193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6481669.1"
                     /translation="MTYSFEGKVAIVTGAGGGLGRCHALELARRGAKVVVNDLGGAMD
                     GTGGSSAAADEVVAEIKAGGGEALANGGSVSDRQGAKSMVVDAMNAWGRVDVLINNAG
                     ILRDRSFGKMELDDFQIVVDVHLMGAVNVTREVWPIMKEQNYGRIVMTTSPTGLYGNF
                     GQTNYGAAKLGQVGFMNSLKIEGAKNNIYTNTIAPVAATRMTEGLIPEGSQVALGPEL
                     VTPAVIFLCSEEAPNGVIVQAQGGNYSVACIVENEGVNLGLDATAEDVGANFEKISDL
                     TGAKPRGMLQLSSM"
     gene            complement(1018..2571)
                     /locus_tag="AC16PP_08320"
     CDS             complement(1018..2571)
                     /locus_tag="AC16PP_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR6481670.1"
                     /translation="MSWETEVGEIERRRHLARQQGGPEGIARQHAKGRLTIRERIGEL
                     LDEGSFREQGKATAIPDYDENDELTGFVPANYVVGFGQVEGRRVVVGGEDFTLKGGSP
                     NAAGLRKSVYAEHLASEYRVPLIRLLEGGGGSVKGSGRKGGTVGEPVYADPRFRIIAD
                     TMTEVPVASGAMGAVAGFPAGRLVASHFSVMTRHTAQVLIGGPALVERALGVRLSKEE
                     LGGVQVHALSGVVDNIAEDEHDAFRQIRRFLSYLPGNVWQRAPRAPSDDDPTRMEQEL
                     LSAVPRDSNAPFDMRVIVEMIVDLGSFFEIGATFGPSQICGLARLDGQPVGVLANDNR
                     HYAGAMTAQAAQKYRRFVEMCDTFHLPIVNFVDQPGFMIGPEAERAGTIRYGMAAVAA
                     AVQATVPWASIQVHKGFGVATAAHYGKGAYVLAWPSVETGALPLEGGVAVAFHREIAA
                     SDDPDAKRAELESGMRAARSPFPRAESFAVHELIDPRETRPMLCEWVDWIQPRLDNLT
                     GPVRFGIRP"
     gene            complement(2584..>3018)
                     /locus_tag="AC16PP_08325"
     CDS             complement(2584..>3018)
                     /locus_tag="AC16PP_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6481671.1"
                     /translation="IALGADIRFAAPDAKMSIMEIKWGLIPDMSITQTLRDVLPLDLA
                     KELTFTGRVLSGAEASQLGLVTHVVEDPLAAALELAREIAGKSPHAVRAGKKLFEESW
                     HADARTGLELEAALQTGLIGSPNQVEAIKANFENRKPDFRDD"
BASE COUNT          526 a         1047 c          908 g          537 t
ORIGIN      
        1 cagaatgagc gtgagtatta cccctgccat atgtgcccgt cggcgcgctg caaagatcct
       61 gccgccagcc tacatgctgc tgagctgcag catgccccgt ggctttgccc cggtgagatc
      121 tgagatcttc tcgaagtttg ctcccacatc ctctgcagtg gcgtccaatc ccaggttgac
      181 gccttcgttc tcgacgatgc aggccaccga gtagttcccg ccctgagcct gaacgatcac
      241 gccgttgggc gcttcttcgc tgcaaaggaa gataacggcc ggggtcacga gctccggacc
      301 gagcgcaacc tgcgagccct cgggaatgag tccttccgtc atccgcgtgg ccgccaccgg
      361 cgcgatggtg ttggtgtaaa tgttgttctt ggcgccctcg atcttcagcg agttcatgaa
      421 acccacctga ccaagcttgg ctgcgccgta gttggtctgc ccgaagtttc cgtaaagacc
      481 cgtgggcgag gttgtcatga cgattcgccc gtagttctgt tccttcatga tcggccacac
      541 ttcgcgggtg acgttgacgg cacccatcag gtgcacgtcg acgacaatct ggaagtcgtc
      601 cagctccatc ttgccgaagg agcggtcgcg cagaatgccc gcgttgttga tcagaacgtc
      661 cacgcggccc catgcgttca tggcatcgac gaccatgctc ttcgcaccct gccggtccga
      721 tacggacccg ccattggcaa gggcctcgcc gcccccggcc ttgatctcgg cgacgacctc
      781 gtctgccgcc gcagacgacc ccccggttcc gtccatggcg ccaccgaggt cgttgaccac
      841 gaccttcgcc cctcgccggg ccagctcgag ggcgtggcat cggccaagac cgccgccggc
      901 tccggtcacg atggcaacct taccttcaaa cgaataagtc atattcttct ccagggctgt
      961 agtcagctaa gcaaacgaga gagccgcgca ttctaatgca atctgggcgc cgggtgatca
     1021 gggccggata ccgaaacgaa cggggccggt gaggttgtcc agacgcggct ggatccaatc
     1081 gacccactcg cacagcatcg gacgcgtctc ccggggatcg atgagctcgt gcacggcaaa
     1141 cgactccgcc cggggaaagg gcgaacgggc agcacgcatt ccggactcga gctccgcccg
     1201 cttcgcgtcc gggtcgtcgg aagcggcgat ctcccggtgg aaggcaacgg caacgccccc
     1261 ttcgagcggc aaagcgccgg tttccaccga cggccaggcc aaaacgtaag cacccttgcc
     1321 gtaatgggcg gccgtggcca ctccgaaacc cttgtgcacc tgaatcgacg cccagggcac
     1381 ggtggcctga acggcggcgg ccaccgccgc catgccgtaa cggatcgtgc ccgctcgctc
     1441 ggcctcaggt ccgatcatga agcccggctg gtccacgaag ttcacgatgg gcagatgaaa
     1501 ggtgtcgcac atctcgacaa agcgacgata cttctgcgcc gcctgggccg tcatcgcgcc
     1561 ggcatagtgc cgattgtcgt tggccagcac ccccacgggt tggccgtcca gcctggccag
     1621 cccgcagatc tgacttggcc cgaaggtcgc cccgatctcg aaaaaactgc ccaggtccac
     1681 gatcatctcg acgatgaccc gcatgtcgaa gggcgcgttg gaatcccgcg gcacggcgga
     1741 aagcagctcc tgctccatgc gggtcggatc gtcgtcgctc ggcgcgcgcg gagcgcgctg
     1801 ccagacatta ccgggcaggt aactcagaaa gcggcggatc tggcgaaagg cgtcgtgctc
     1861 gtcctccgcg atgttgtcca ccacgccgct cagcgcatgg acctgcacgc cgccgagctc
     1921 ttccttggac aggcgcacgc ccagcgcccg ctcgaccagg gccggaccgc cgatgagcac
     1981 ctgagccgtg tgccgggtca tcaccgagaa gtgggacgcc accagccggc cggccggaaa
     2041 tccggcgacc gcccccatgg cccccgatgc caccggaacc tcggtcatcg tgtcggcgat
     2101 gattctgaag cgcgggtcgg cgtacacggg ttcaccgacg gttcctcctt tacgacccga
     2161 tcccttcacg ctgcccccgc caccctcgag gagccggatc agcggcacgc ggtactcgct
     2221 ggcaaggtgc tcggcgtaga cgctcttgcg caatccggcc gcgttcggag agccgccctt
     2281 gagggtgaag tcctcgccgc ccaccacaac gcggcgtccc tcgacctggc cgaaacccac
     2341 cacgtagttg gccggaacga acccggtcag ctcgtcgttc tcgtcgtagt cgggtatggc
     2401 cgtcgccttg ccctgctcgc gaaaactgcc ctcgtccaga agctcgccga tccgttcacg
     2461 aatggtgagc cggcccttgg cgtgctgacg tgcaatgccc tccggcccgc cctgctggcg
     2521 cgcaagatgg cggcggcgtt cgatctcacc aacctcggtt tcccagctca tcgtgcggtt
     2581 ccgtcaatcg tccctgaaat cgggcttcct gttctcgaag ttcgccttga tggcttcgac
     2641 ctggttgggc gagccgatca gacccgtctg cagggccgcc tccagctcca gccccgtgcg
     2701 cgcatccgcg tgccagctct cctcgaacag cttcttcccg gctcgaacgg catggggcga
     2761 cttcccggcg atctcacggg cgagctccag ggcggcggcc agggggtcct ccacgacgtg
     2821 agtcaccagc ccgagctgcg aggcttccgc tccgctgagc acacgcccgg taaaggtcag
     2881 ctccttggcg agatctagag gcagaacgtc acgcagggtc tgggtaatgc tcatgtccgg
     2941 aatgaggccc catttgatct ccatgatgga catctttgca tcaggagccg cgaagcgaat
     3001 gtcagcaccc agcgctat
//
LOCUS       DBNHKV010000263         2678 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_674526, whole genome shotgun sequence.
ACCESSION   DBNHKV010000263 DBNHKV010000000
VERSION     DBNHKV010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_674526"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="AC16PP_08330"
     CDS             <1..1077
                     /locus_tag="AC16PP_08330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6481672.1"
                     /translation="EGRVNRVLHADEHRILFTGWFGNPQEQHLYSVSASAASGPEEPV
                     RLTAEPGWHEVTVDLRGEQFLDRFSAVDRQPSCRVRKLEGPQVNTIGQELADGLSNYA
                     PYLTHHVTPQLGTVVCEDGQTLHYRLTRPSNGSRQKSPVIVHVYGGPGVQRVRNEWAP
                     ALLQLFVQHGFGVFELDNRGSGNRDRGFEEPIHRQLGKVEVADQVLGADFLRSLDWVD
                     PERIGVFGHSYGGYMTIMCLAQAPASFKAGVSVAPVTDWHLYDTHYTERYLGHPEDNA
                     SGYAASSVFPHLDGIKGRLLLIHGMADDNVLYTNSTMLYRALQARHFPFEMMAYPGSK
                     HSLQERDVSIHRFNLILDFFERAL"
     gene            1074..1541
                     /locus_tag="AC16PP_08335"
     CDS             1074..1541
                     /locus_tag="AC16PP_08335"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6481673.1"
                     /translation="MSERTTNVSVRSAGSGDASLLIEFNLAMAMESEDKGLDRATLAA
                     GVEHLIEHPLDGFYLVAELAGVPAGSLMVTYEWSDWRAGRFWWIQSVYVKPEYRRKGV
                     YSALHRSVRERALSAPDTCGIRLYVERENAGAQATYASLGMSETAYRLYEEEF"
     gene            complement(1538..2299)
                     /locus_tag="AC16PP_08340"
     CDS             complement(1538..2299)
                     /locus_tag="AC16PP_08340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009406818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR6481674.1"
                     /translation="MLVDSHCHLNYLDDPDSRLQAARLAGVDGFLCIGVDKDGIAAVL
                     DIAERHLDVWASVGQHPEAAESPPDWVSDYLTHPRVVAVGETGLDYHHGRQAHIQRLQ
                     RERFAWHLEIAGVAGLPVVVHTRDAVADTADLLRAHRDVGGVLHCFTETWDLAKSALD
                     LGYYISISGIVTFRNGENVREVASRVPRDRLLVETDCPWLAPAPHRGRTNEPAFVRDT
                     ATFLADHLNWDRDALAAQTSENFFRLFACGPQTAA"
     gene            complement(2306..2656)
                     /locus_tag="AC16PP_08345"
     CDS             complement(2306..2656)
                     /locus_tag="AC16PP_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR6481675.1"
                     /translation="MAARRGARNGILSLAIKDKAVLYAAYMPFVKNGGLFIPTKKDYE
                     LGDEVFMLLNLMDEAEKIPVAGKVVWVTPSGAQGNRAAGIGVQFNDQDDVARSKIETY
                     LAGALQSDRHTHTM"
BASE COUNT          498 a          843 c          855 g          482 t
ORIGIN      
        1 gagggaaggg taaaccgcgt gcttcacgcg gatgagcacc gcatcctgtt caccggctgg
       61 ttcggcaacc cccaggagca gcatctctac tcggtcagcg cgtccgctgc ttcggggccg
      121 gaggagcctg tcaggttgac ggcggagcca ggctggcatg aggttaccgt cgacctcagg
      181 ggcgagcagt ttctcgaccg cttcagcgcc gtcgaccggc agccgtcctg tcgggtcagg
      241 aagcttgagg ggccccaggt gaacaccatc gggcaggagc tcgcggacgg gctttcgaac
      301 tacgcgccct acctcaccca ccacgtgaca ccgcagctcg gcaccgtcgt ctgcgaggac
      361 ggccagaccc tgcattaccg gctcacgcga ccatcaaacg ggagccgaca gaagagtccg
      421 gtgatcgtgc atgtgtacgg cgggccaggc gtgcagcggg tgcgtaacga atgggcgcct
      481 gcgctgctgc agcttttcgt ccagcacggg ttcggcgtgt tcgagctgga caaccggggt
      541 tccgggaacc gcgaccgcgg gttcgaagag cccattcacc gccagctggg caaggtcgag
      601 gtggcagacc aggtgctcgg cgccgatttc cttcgctcgc tcgactgggt tgatccggaa
      661 cgcattgggg ttttcgggca cagctacggg ggctacatga ccatcatgtg cctggcccag
      721 gcccctgcgt cgttcaaagc cggcgtttcg gtcgcaccgg tgaccgactg gcatctttac
      781 gacactcact acacggaacg ttatctcggg caccccgagg acaatgcttc cgggtacgct
      841 gccagcagcg tatttcccca tctcgacggg atcaaaggca ggctgctgct cattcacggg
      901 atggccgatg acaacgttct ctacacgaac agcaccatgc tctaccgggc gcttcaggcc
      961 cggcacttcc cctttgagat gatggcctac ccggggtcca agcattcact tcaggagcgc
     1021 gatgtctcca ttcatcgttt caatctgatt ctcgatttct tcgagcgggc gctgtgagcg
     1081 aaagaacaac caacgtcagc gtaaggtcgg cgggcagcgg cgacgcaagc ctgctgatcg
     1141 agttcaacct ggccatggcg atggagagcg aggacaaggg cctggatcgc gcgacgctgg
     1201 ccgcgggcgt ggagcacctc atcgagcacc cgctcgacgg gttttatctg gtagcagagc
     1261 ttgccggggt gccggccggc tccctgatgg tcacctacga gtggagcgac tggagagcgg
     1321 gacgattctg gtggatccag agcgtgtacg tgaaaccgga gtatcgccgc aagggcgtct
     1381 actcggcgct gcatcggagc gtgcgcgaac gggcgctttc agcacccgac acgtgcggca
     1441 tccggctcta cgtcgagcgg gaaaacgcag gcgcccaggc aacctatgcc agcctcggca
     1501 tgtcagagac cgcctaccgg ctttacgagg aagaattcta ggccgccgtc tgcggcccac
     1561 aggcgaagag gcgaaagaag ttttcgctgg tctgcgctgc cagcgcgtcc cgatcccagt
     1621 tcaggtggtc cgcgagaaac gtcgccgtgt cacgaacgaa ggcgggctcg ttggttcggc
     1681 cacggtgcgg tgccggcgcc aaccacgggc agtcggtttc tacgagcaga cggtcacggg
     1741 gcaccctgct cgccacctcg cggacgttct caccatttcg aaacgtcacg atgccggata
     1801 tcgagatgta gtacccgagg tcgagcgcgc tcttggcgag gtcccaggtc tccgtaaagc
     1861 agtgcagaac gcctcccacg tcccgatgcg cccgcaggag gtcggccgtg tccgccaccg
     1921 catcgcgggt atgcacgacg acgggcagtc cagcgacgcc cgcaatctcg agatgccagg
     1981 caaaccgttc ccgctgcagg cgctggatat gcgcctggcg accatggtgg tagtccaggc
     2041 ccgtttcccc cacggcgacg acccggggat gagtcaggta gtcgctgacc cagtccggcg
     2101 ggctctcggc ggcttcagga tgctgtccga cgctcgccca gacatcaagg tggcgctcgg
     2161 caatgtcgag cacggcggcg atgccgtctt tatccacccc gatgcacaga aacccatcga
     2221 cgccggcaag ccgcgccgcc tgcagacggg agtccgggtc atcgagatag ttcaggtgac
     2281 agtgagaatc gaccagcaag gccgttcaca tggtgtgagt gtgtctgtcg gactgcagcg
     2341 caccggcgag gtaggtttcg atcttggagc gggcaacatc gtcctgatcg ttgaactgca
     2401 cgcctattcc ggccgcgcga tttccctgcg cgcccgaggg cgtcacccag acgaccttgc
     2461 cggccacggg aatcttttct gcttcgtcca tgagattgag aagcatgaaa acctcatcgc
     2521 cgagctcgta gtccttcttg gtgggaatga acagcccccc gtttttcacg aaaggcatgt
     2581 aagcggcata aagcaccgcc ttgtccttga tcgcgaggga gagaattccg tttctagcgc
     2641 cgcgacgagc cgccatctgg acaatacctt gtgccccg
//
LOCUS       DBNHKV010000264         3101 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1525605, whole genome shotgun sequence.
ACCESSION   DBNHKV010000264 DBNHKV010000000
VERSION     DBNHKV010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1525605"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="AC16PP_08350"
     CDS             <1..814
                     /locus_tag="AC16PP_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6481676.1"
                     /translation="SLKLKQRGFQSDTAIAQTKARLAAAEAKLARSALEVSRTEIRAP
                     FDGVVETIHQDVGDYVNVSAPCVTVVDADPMLLIGRVAEKDVHRLLLDQNATGLLSNG
                     TTVTGPVTFIGQQSDPATRTYAVEIQIPNPERAIRSGITTEIRIPVAETMAQKVSPAV
                     FTLDDAGHIGIRIVNADYRVELHPVTIIREAPDGVWVSGLPNVATVITVGQELVVAGE
                     MVEVDFEPAATLPAAAPAERAVAPEADGPDSGPSAANLTSRTSSTEALSRAT"
     gene            811..>3101
                     /locus_tag="AC16PP_08355"
     CDS             811..>3101
                     /locus_tag="AC16PP_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6481677.1"
                     /translation="MNSLIDAAISRSRTTLLVMFMVVIAGLIARGAIPIASEPRIEVP
                     FFFVGVVHEGISPEDAERMLVMPMEIELRKVEGVKELTAYASEGSATILVEFDVSHDL
                     DQALLDVREAVDRARPELPSTAEEPYVREETTEDFPIVQVNLVGEGVPERILYNLAIE
                     LRDDIEAIPSVLEAELKGHREEMLEAVIDPAALEAYAISNEQLITTIVRNNRLIPAGS
                     IDTGEGRFAVKIPSIIERAEDVLDLPVRASGDTVVTLDDVAVVRRTFKDRTSYARVNG
                     NAALSLQITKRANANIIDTIDAVKAVVERHRPDLPNRVDVFFSQDQAPFAEGQVTELQ
                     GNILTALALVMVLVVAAMGFRSGVIVGLAIPVSFLFSLIFIYLLGYTFNFMVMFGMLL
                     GLGMLIDGAIVVTEYADRKMTEGFERRAAYAMAAKRMFWPVTASVATTLAAFLPLMFW
                     PGIPGKFMSYLPVTVLTVLSGSLIYALIFGPVLGSLFGKAGARDAKAQRTLNELEDGD
                     PTRLKSVTGWYARLLTITSRHALATLSGILVILVTIFWGYGAYGKGMVFFSDSDPQFA
                     QVAVRARGNLSADEIDALVREVDSEILEVSGIRTVNTYTLLSGGSTRGGFDRVGSMFL
                     ELHPESERDRKGAEIFAEIRQRTAGLAGIAVEVQKLEQGPPVGKPVQIQFSSYDRELL
                     EPAVTRVREYMETLPGLLDIDDTRSLPGIEWKLSVDRAQAALYGADVSQVGIAVQLVT
                     NGVKVGEYRPERADDAVDIRVRYP"
BASE COUNT          564 a         1050 c          967 g          520 t
ORIGIN      
        1 gagcctgaag ctcaagcagc ggggctttca gtccgatacc gccatcgccc agaccaaggc
       61 gcgtctagca gccgccgaag ccaagctcgc ccgaagcgct ctggaggtct cccgcacgga
      121 gatccgcgcg cccttcgacg gggtggtgga aaccatacac caggacgtgg gcgactacgt
      181 caacgtgagc gcgccctgcg tcaccgtcgt cgatgccgac cccatgcttt tgatcgggcg
      241 ggtcgccgag aaagacgtgc accggctcct tctggaccag aacgcgaccg ggctgttgag
      301 taacgggacg accgtcaccg gccccgtcac cttcatcggc cagcagagcg atcccgctac
      361 ccgcacctac gcggtggaga tccagattcc caatccggag cgggcgatcc gctccggaat
      421 caccaccgag attcgtatcc ccgtggccga gaccatggcc cagaaagtca gccctgccgt
      481 cttcacgctc gacgatgcgg ggcacatcgg cattcgcatc gtcaacgccg actaccgcgt
      541 cgagcttcac cccgtgacca tcatccgaga agcgccagac ggcgtgtggg tgtccggcct
      601 tcccaacgtc gccaccgtga taactgtcgg ccaggagctg gtcgtggccg gagagatggt
      661 ggaggtagac ttcgagcctg ccgccaccct gccggccgcc gcccccgcgg agcgcgccgt
      721 cgcgccggaa gccgacggcc ccgacagcgg accgtcagcc gccaacctca ccagccggac
      781 gagctcgaca gaagccctct cacgcgcgac atgaacagcc tgatcgacgc ggccatctcc
      841 cggtcgcgca ccaccctcct ggtcatgttc atggtcgtga tcgcgggcct gatcgcccgc
      901 ggcgcgatac cgatcgccag cgagccccgc atcgaagtgc ccttcttctt cgttggcgtg
      961 gtccacgagg gcatctctcc ggaagacgcc gagcgcatgc tcgtcatgcc catggagatc
     1021 gagcttcgta aggtagaggg cgtcaaggag ctcaccgcct acgcatccga aggatctgcg
     1081 accattctgg tcgagttcga cgtcagccac gatctggacc aggcgctgct cgacgtccgc
     1141 gaagccgtgg accgggcgcg accggaactt ccgagcaccg ccgaagagcc ctacgttcgc
     1201 gaggagacca ccgaggactt ccccatcgtg caggtcaacc tggtcggcga gggggtgccc
     1261 gagcgcatcc tctacaacct ggccatcgag ctgcgcgacg acatcgaggc gatcccaagc
     1321 gtgctggagg cggagctgaa ggggcatcgc gaggagatgc tcgaagctgt gatcgacccg
     1381 gccgctttgg aagcctacgc catttcgaac gagcagctga tcaccacgat cgttcgaaac
     1441 aaccggctga tcccggccgg cagcatcgac accggcgaag gcagattcgc cgtcaagatt
     1501 cccagcatca tcgaacgcgc agaggacgtt ctggacctgc cagtacgggc cagcggagac
     1561 acggtggtaa ccctcgacga cgtagccgtc gtgcgccgca cgttcaagga ccggaccagc
     1621 tacgcgcgcg tgaacggcaa cgcggcgctt tcattgcaga tcaccaagcg cgccaacgcg
     1681 aacatcatcg acaccatcga tgcggtcaaa gccgtcgtcg agaggcatcg gcccgacctg
     1741 ccgaaccggg tcgacgtctt cttctcccag gaccaggccc ccttcgccga gggacaggtc
     1801 accgagctgc agggcaacat cctgactgcg ctcgcgctgg tgatggttct ggtcgtggcc
     1861 gccatgggtt tccgcagcgg cgtgatcgtc gggctggcca taccggtgtc gttcctgttc
     1921 tcgctgatct tcatctacct gctggggtac acgttcaact tcatggtcat gttcggcatg
     1981 ctcctcggtc tgggcatgct catcgacggg gccatcgtcg tgaccgagta cgcggaccgc
     2041 aagatgaccg aaggcttcga gcgccgcgcg gcttatgcca tggccgccaa acggatgttc
     2101 tggcccgtca ccgcatccgt cgcgacaacc ctggcagcct tcctgccgct gatgttctgg
     2161 ccgggaatac cgggcaagtt catgagctat ctgccggtca ccgttctcac cgtgctttcg
     2221 ggatcgctga tctacgcgct gatattcggc cccgtgctgg gttccctgtt cggcaaggcg
     2281 ggcgcgcgcg acgccaaagc gcagaggacg ctcaacgagc tggaagacgg cgatcccacg
     2341 cgtctgaaga gcgtgaccgg ctggtacgcg cggctgctga ccatcaccag ccgacacgcc
     2401 ctggccaccc tctccggcat cctggtcatc ctggtcacca tcttctgggg gtacggcgcc
     2461 tacgggaagg gcatggtgtt cttctcagac agtgatcccc agttcgccca ggttgccgtg
     2521 cgcgcccgcg gcaacctgtc agccgatgaa atcgacgctc tggtccgcga ggtggacagc
     2581 gagatcctcg aggtttcggg catacgaacc gtcaacacct acacgctctt gagcggcggc
     2641 tccactcggg gcggtttcga ccgcgtgggc tccatgttcc tcgagctgca ccccgagagc
     2701 gagcgcgacc gcaagggtgc ggaaatcttc gcggagatac ggcaacgcac cgccggcctg
     2761 gccggcattg ccgtcgaggt gcagaagctc gagcagggcc ctccggtggg caaaccggtg
     2821 cagatacagt tttcttccta cgaccgtgag ctgctggaac ccgccgttac gagggtgcgc
     2881 gagtacatgg aaaccttgcc cgggctgctg gacatcgacg acacgcgatc cctgcctggc
     2941 atcgaatgga aactgtccgt ggatcgcgcc caggccgccc tgtacggcgc tgacgtttcc
     3001 caggtcggca ttgccgtgca gctggtcacg aacggcgtga aagtcggcga gtatcgtccg
     3061 gagcgcgcgg acgatgcggt ggacatccgg gtcaggtacc c
//
LOCUS       DBNHKV010000265        15139 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1454721, whole genome shotgun sequence.
ACCESSION   DBNHKV010000265 DBNHKV010000000
VERSION     DBNHKV010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 15139)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15139)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15139
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1454721"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="AC16PP_08360"
     CDS             complement(<1..1181)
                     /locus_tag="AC16PP_08360"
                     /inference="COORDINATES: protein motif:HMM:NF014269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR6481678.1"
                     /translation="MPVLPVLPLKDTVVYPQIVRPLGVGRRRSLAALNHAVEGPREII
                     TVAQHDGANEEPGLTDLYAVGCIASISRVENRDGGAQVIVQGTRRVRLTGIAEDEGAF
                     LAAQYEELPGLGMNDLGTDGPAVDATLRENLQLAQRIAEIMDSENGAQIYQQLVGSIS
                     DPITQMYRIASLAPGIELIQEQRILEADTTLALMEAIHELLGHELQVTELRRQIAERA
                     RDDMDQQQREHVLRQQKRAIEQALGESGEDDDIAELKGQLDEARLPEPVQAEVDRELK
                     RLARMSANAADYQVARAYLELIGELPWHQETEDQLDLKHAREILDEDHYGLEDVKERI
                     LESLAVMQLNPGAKAPILCFVGPPGVGKTSLGQSIARAMGRKFERLSLGGLHDEAELR
                     G"
     gene            complement(1192..2259)
                     /locus_tag="AC16PP_08365"
     CDS             complement(1192..2259)
                     /locus_tag="AC16PP_08365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6481679.1"
                     /translation="MEFGLSEEQTLLQDSLNGFLDANCPLDRVRRFAEEPVAADLHSG
                     LAELGVFGLLVPEEAGGVGLGCFEAALVAEALGSHTAPCAFLGSAVMAPLALTLAGNE
                     PTDTLSRLATGELTVGVAVAEFVGARADGGITADGETLSGKALSVLDFDAQAYLVADA
                     AGSTYLVDAGAPGVFAEALPTIDKTRRTGALTLNGAPAQLICDDPDVLARVIDAGRVM
                     LAADTLGAAQNMLDQAVEYAMQREQFNRPIGSFQAVKHMCAEMAASLEPCRAMVWYAS
                     HALDDLPAEASLCACHAKAHLSEVGKFVAKTATEVHGGMGFTDLLGLHYWFKRIGYNR
                     QMLGAPELLREQAARLQGLCA"
     gene            complement(2269..3459)
                     /locus_tag="AC16PP_08370"
     CDS             complement(2269..3459)
                     /locus_tag="AC16PP_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6481680.1"
                     /translation="MDLSYGPHYEEFREEVRSFLADHAGSAPRGEHIRSEKTLAWQHL
                     LIENGYAARTIPREYGGYGAAPDILESRIIAEEFAAARVSSGLGGQGISMLVPTLLEM
                     GTQEQKTTFIPPTLRGEMLWCQGYSEPGAGSDLASLSTRAELDGDEWVINGQKIWTST
                     AHIADWMFCLVRTEPDAPKHQGISFLLLRMDTPGIEVRPLVDMTGAANFNETFFTDVR
                     VPKDQIVGRRGEGWLVANAILGHERDSLGDPNVMLSRFNALTELMKTETLGGERLIDH
                     PVFRDRLMQIQGRVMALSYNDLRLLSAKVNAADARLARMIVKLQGTELRHDLEGLAVD
                     AMGELGLAYGDNPYLRDHGSWQSQYMFYLGLIIGGGTSQIQKNIISERGLGMPKEPKY
                     EKQA"
     gene            complement(3559..4566)
                     /locus_tag="AC16PP_08375"
     CDS             complement(3559..4566)
                     /locus_tag="AC16PP_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HIR6481681.1"
                     /translation="MAKSPSTALVMHPVVRDELITHAHMERLRKTVNFIDEEPFRDLD
                     AIGGALGQVELLITSWGCPRVDADCLARLPRLKLIAHLAGSVKGFLDDIVWRRGILVT
                     NAVAANAVPVAEYTLAAILFANKRVFQLNRVYLEHREHRPPWTKEAPDVGNYHKSVGI
                     VGASHVGRLVMEHLRRFDIRLLLYDPFVTPLAARDLGATKVGLSELLSQSDTVSLHAP
                     LLKETRHMIGARELALMHDGATIVNTARGALIDQAALEAELTRGRLYAVLDTTDPEEL
                     PPESPLYGLPNVFLTPHIAGSLGTETQRLTDCIIEELERYGRGVPLKYRVRREQLDRL
                     A"
     gene            4627..5253
                     /locus_tag="AC16PP_08380"
     CDS             4627..5253
                     /locus_tag="AC16PP_08380"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF020341.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR6481682.1"
                     /translation="MDRARALAMIQTMAEMQESHNRHVHPEWIEQGYAYYRAVWVECA
                     ELLDHFGWKWWKKQQPDLGQVKLEVVDIWHFGLSDLIRAGTLDTAAPVLLEVSASEPT
                     DLEGFRLAVEALAADTLSSRAFAMAPFAAVLESLPMSFAELFEMYVGKNVLNNFRQDH
                     GYKTGEYQKLWQGREDNEHLIDILGELDCPAESVPAELYRALQARYPV"
     gene            complement(5241..5750)
                     /locus_tag="AC16PP_08385"
     CDS             complement(5241..5750)
                     /locus_tag="AC16PP_08385"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HIR6481683.1"
                     /translation="MTGIEVVEGDITTQSVDAIVNAANESLLGGGGVDGAIHRAAGPG
                     LKAECRRLGGCPTGEARITGGYDLPAAHVIHTVGPIWRGGDAGEAALLTACYRNSLDL
                     ARRHHIRSIAFPAISTGAFGYPLAEATAIAVATVGSFIATTPLLARFVCFDARTARAY
                     REELAAQTG"
     gene            5853..6707
                     /locus_tag="AC16PP_08390"
     CDS             5853..6707
                     /locus_tag="AC16PP_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HIR6481684.1"
                     /translation="MTLLASSIARLDADARWALRGHELFFASLAVGEAQRAEGAAAEL
                     LASVEHGARALGVGKIGIDRPQDEALVAWYQQRGYYPGAYPRGYLGEGRGEGPALLAK
                     DLNAAREMEVFFRGWAPELIGTLLFVIDGSRVLLIDKKTGHGAGKINAPGGKLEPGES
                     PVACAVREVREEIGITVAAPRLHAELAFADLRGSQWFGYVFVARAYRGEPVETAEARP
                     SWHPLNAIPYGRMWEDDRVWLPRVVAGERVRGRFLFRAGRLLAHALEGLGEVDGSEAE
                     HRGGCHGR"
     gene            6697..7323
                     /locus_tag="AC16PP_08395"
     CDS             6697..7323
                     /locus_tag="AC16PP_08395"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6481685.1"
                     /translation="MAADLNWLDSCRRLRAIAQTGLAYSRDPYDRDRFQELRLIAERM
                     LAWLSDAHPDRVAEVFLPERGYPTPKIDVRAGVFRGDSVLLVKERADGRWALPGGWGD
                     EGDSPRSSIEREVLEESGYEVRAARLVAIKDRHLHPYEKHSMLRVYKLFFLCDLLGGE
                     PRASLETESAEFFPITRLPELSLGRTLPDDVHLLNAYRAEPDRDVYLD"
     gene            complement(7445..8752)
                     /locus_tag="AC16PP_08400"
     CDS             complement(7445..8752)
                     /locus_tag="AC16PP_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase family protein"
                     /protein_id="HIR6481686.1"
                     /translation="MTDLNWPFLGGAALEIESASGVHLTTRDGRRILDAAGGAIVVNI
                     GHGRRRVADRIAEAALTCSYVVPPWVTPSRRAMVEALEANWLDAAFTRLHFTSGGSEA
                     VEAAMKLALQYHAARGHGSRNQILSRAVSYHGTTVATTAVSGHAARRRGLEHGLPRPP
                     TIPTPDALRCPLGANHPDAGAYYAGAVREGIVEAGPENVAAFLAEPITGASGGAIVPP
                     ADYWPRVSEICREFGVLLILDEVMTGFGRTGTNFAYQQWEILPDILIAGKGLAGGYAP
                     LGGVFATEPVGAAIGAAGLQVMFHTFAAHPTACAAAAEVLDILVEEQLVARADRMGTM
                     LLERLQDAFREHPHVSDVRGRGLLQAIEIVSDRATLTPFPMEAQITQRVVDAALERGV
                     FFYAGGTGEMRDIICLGPPFVIEERHVDEMVSVLRQSVDAVTR"
     gene            complement(8757..10394)
                     /locus_tag="AC16PP_08405"
     CDS             complement(8757..10394)
                     /locus_tag="AC16PP_08405"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481687.1"
                     /translation="MTRHLLFAAAGLLLAACSTVSIDGPTAPRGDPAAGRLTLPSGRT
                     VWVYPNDLAFTGQARYEWPDGRAYAGGWLSGRPHGTGLMRSDDGERYSGMWNEGRRHG
                     HGELTNPNGDHYVGSFVNGLREGAGTQKSQDGFYRGEWAGDLPNGEGRFNANDGSLYQ
                     GQWLDGERAGTGTYTDASGNRYEGEWLADVPHGFGILTEQDGSEYDGRWTAGSRDGYG
                     ASRNALDMAYEGTWNAGNYHGFGILLRPDGSSYEGEWHHGKRQGQGRETDPEGGFHDG
                     AWENNQPLGPGTRRDVAGVEIAGMWTGSRVRTGLVKLPTGLEYAGPLFKDRNRGVSAP
                     LQEWLLTQASRGDPYAQLYLGRAFDDLGGTSADAARAAQWYALAAAAGVAEARYRLAL
                     LNIEDNPPRAVELLAAAAGQGHARANAALADCYLAGKIVPRNAARAIAYLETAMDAGS
                     VAARNTLARVLATTTEDGLRDGARAVALIRPLALYTESWWQLDTLAAALAETGAYGDA
                     VTALQLALERADQISPRDVQQLVDHLAAYRAETPLRE"
     gene            10473..11507
                     /locus_tag="AC16PP_08410"
     CDS             10473..11507
                     /locus_tag="AC16PP_08410"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6481688.1"
                     /translation="MTASKDDGSIEGVDIPRVTHWLAGRIAGLRPPLEFSLIAGGHSN
                     LTYRFADQADAEYVLRRPPLGHVLESAHDMAREHRIVSALAGTEVPVAPTHGLCEDRD
                     VNGAPFYVMSYVRGAVLHDADAARELDPAERHALGEDVINVLARLHALDPDAVGLGTL
                     GRKEAYLARQLKRWKKQWEASKTHEIPEMEETARLLEENMPEQIGAAIVHGDYRLGNM
                     IVADGRILAVLDWELCTLGDPLADVGYLLNNWIQPEDGAGELVPTAAGGFPSREALCD
                     GYTAATGRDLSGINYYRAFSHWRLAAIGQGVYKRYLVGAMGERPDMDLDTYKASVQQR
                     AAAALELLTA"
     gene            complement(11504..11974)
                     /locus_tag="AC16PP_08415"
     CDS             complement(11504..11974)
                     /locus_tag="AC16PP_08415"
                     /inference="COORDINATES: protein motif:HMM:NF025701.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HIR6481689.1"
                     /translation="MTRSRTILGALLVAALYGCSGRDDVSLVEVLNHDQCRMSQAGTE
                     VVSIEDVARIRGGRLLSAPDEPATTPTDLLLVAVSRGQQPTPGYGFELVAGTMKGDAV
                     TVRLAWNQPPADAVLAQVITNPCIVIGLEGEGFSRLRVIDQDDTPLGEIVLPGG"
     gene            complement(11971..14838)
                     /locus_tag="AC16PP_08420"
     CDS             complement(11971..14838)
                     /locus_tag="AC16PP_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR6481690.1"
                     /translation="MIRQALPKTVWTVTCVLTLTACGGGGGGSDTPTPAPANRSPAAS
                     FTATPTEGTVPLSVDFDASGSSDADGRIASYAWTFGEPPGASSTSGVNTSHTYEEAGT
                     YTVELTVTDDDGATGSTTRTVTVEGPVASISGTVRILASSAIDSDVNDVGSTPAPNND
                     FASAQPLPNPVTLGGFANVAGVGVGDEPERGKLFQDGDPGDFYAMTMVGNEFIYLNIA
                     DPENAEQELLLYDSQQNVISADGGPGEVQVAEVPAAGDYFIEVRPIAGASNYVLTVGQ
                     DLVPERATTMIDPFLPREIILRTTDDEIAAEYGLSMLAQSGPRMLLKAGPATAALRHI
                     DRRAPFDASWTEDQQDRYATLAVIEALGRDPRVELAEPNLIRKPHREPGDSFYRSQWH
                     YQAINLPLAWDITTGDGDVIVAVVDTGIRADHPDLDEKVIAGFDFIANLQRAADGDAR
                     DDDPTDPGDGAFGGSSSFHGTHVAGTVAAETSASVTPASGVAGVAWEAKIMPLRVLGV
                     GGGTSFDVIESMRWAARLDNVSGTLPDRAADVINLSLGGGGFLESEQQAIDEIRAAGT
                     IIVASAGNDASASPSYPAAYDGVVSVSATTIENNPAAYSNFGTTVDVAAPGGSNVTDV
                     NGDGIGDGVISTMADDSDPSSLRFGYASLQGTSMAAPHVAGVVALMKAVHPGLTPIQF
                     DNALASGRITDDLGAPGRDDRYGWGLINAQKAVLAALDLASNPPGPTLAVSASSLTFG
                     AALSSQSLRLENVGSGTVTVQSIGTAASWLDVAPSSVLPGGMGSYTVTVDRTGLTPGS
                     YTGAIEIVTDAVNPTATVTATMRVFTTSPDANAGVHYVVLVDAAGETLPSPQNVLEAT
                     NGEYAYRLDGVAPGDYRIFAGTDSDDDAFLCDAGEACGTYGTLDSPAVVTVDGEDLTG
                     IDFTSEFRVNLTNLSANADVSGQVAPEAVRLPGKADGGAPR"
BASE COUNT         2369 a         4938 c         5194 g         2638 t
ORIGIN      
        1 tgtcctctga gctccgcctc gtcgtgcaga ccaccgaggc tcaaccgctc gaacttgcgc
       61 cccatggccc gggcaatgga ctggcccagg gacgtcttgc ccaccccggg cgggcctacg
      121 aagcagagaa tgggcgcttt cgcgccaggg ttcagctgca tgacggccag agattcgagg
      181 atgcgctcct tgacgtcctc cagaccgtag tgatcttcgt ccagaatttc cctggcgtgc
      241 ttcagatcga gctgatcctc ggtctcctga tgccagggca gctccccgat tagctccaga
      301 taggcccggg cgacctggta gtcggcggcg ttcgccgaca tgcgggcgag ccgcttcagc
      361 tctctgtcca cttccgcctg aacgggctcc ggcaggcggg cctcgtcgag ctggcccttc
      421 agctcggcaa tgtcatcgtc ctcccccgac tcccccaggg cctgctcgat ggcgcgtttc
      481 tgctggcgca ggacgtgttc acgctgctgc tgatccatgt cgtcccgggc ccgttccgcg
      541 atctgcctgc gcagctcggt gacctggagc tcgtgtccca gcagctcgtg gatggcttcc
      601 atcagggcga gcgtggtgtc ggcctcgaga attcgctgct cctggatcag ctcgatcccc
      661 ggggcgaggg acgcgatgcg gtacatctga gtgatgggat cggagatcga gcccaccagc
      721 tgctgataga tctgcgcgcc gttctcgctg tccatgatct ctgcgatgcg ctgggcgagc
      781 tggaggttct cccgcagggt agcgtccacg gccgggccgt cggtcccgag gtcgttcatg
      841 ccgagacccg gcagctcctc gtactgcgcg gcgaggaacg cgccctcgtc ctccgcgatt
      901 ccggtcaacc gtacccggcg cgtcccctga acgatcacct gggcgccgcc gtcgcgattc
      961 tccacgcgac tgatgctggc gatgcagccg acggcgtaga gatccgtcag gcccggctcc
     1021 tcgttggcgc catcgtgctg ggcaacggtg atgatctccc tgggcccctc caccgcgtgg
     1081 ttcagggccg ccagagagcg acgccgaccc acgccgaggg ggcggacgat ctgcgggtag
     1141 acgaccgtgt ctttaagggg caggacgggg aggactggca tggcgttctc gtcaggcgca
     1201 caggccctga aggcgggctg cctgctcgcg caacaactcc ggagctccga gcatctggcg
     1261 attgtagccg atccgcttga accagtagtg gagtccgaga aggtccgtga agcccatgcc
     1321 gccgtgtacc tcggtcgccg tcttggcgac gaacttgccg acctccgaaa ggtgggcctt
     1381 ggcgtggcag gcgcacaggg aggcttcggc gggaaggtcg tcgagcgcgt gggatgcgta
     1441 ccagaccatg gctcggcacg gttcgaggct cgcggccatc tctgcgcaca tgtgcttgac
     1501 ggcctgaaac gagccgatgg gccggttgaa ctgctctcgc tgcatcgcgt attccaccgc
     1561 ttgatcgagc atgttctggg cagcgccgag ggtgtccgct gcgagcatga cccgtcccgc
     1621 atcgatcacc cgggcgagca cgtccgggtc gtcgcagatc agctgtgccg gcgccccgtt
     1681 cagcgtgagc gcgccggtgc ggcgggtctt gtcgatggtt ggaagcgctt cggcgaacac
     1741 cccgggcgct ccggcgtcga cgaggtaggt gctccctgcc gcgtccgcga ccaggtaagc
     1801 ctgggcatcg aaatccagaa cggagagcgc tttgccggag agcgtctccc cgtcggcggt
     1861 gatgccgcca tccgcccgcg cgccgacgaa ctcggccacg gcgaccccga ccgtgagttc
     1921 tcctgtcgcc agtcgggaca gggtatccgt cggctcgttg cccgccagcg tgagggccag
     1981 cggcgccatc acggcggaac ctagaaaagc gcaaggggcg gtgtggctgc caagggcctc
     2041 ggcgaccagc gccgcctcga agcagcccag gcccaccccg cccgcttcct ccgggacgag
     2101 cagcccgaag acgccgagct ccgccaggcc ggaatgaagg tcggccgcca caggctcctc
     2161 ggcaaagcgg cgtaccctat ccagagggca gttggcgtcc aggaagccgt tcaagctgtc
     2221 ctgcaacagt gtctgctctt cggaaagccc gaactccatg gcggcctcct aagcctgctt
     2281 ttcgtatttg ggttctttcg gcatgccgag cccgcgttcg ctgatgatgt tcttctggat
     2341 ctgcgaggtg cccccgccga tgatcagccc caggtagaac atgtactggc tctgccagct
     2401 gccgtgatcg cgcaggtagg ggttgtcgcc gtaggccagc cccagctctc ccatggcgtc
     2461 cacggccaga ccctcgaggt cgtggcgcag ctcggtgccc tgcagcttga cgatcattcg
     2521 tgcgaggcgg gcatccgctg cgttgacctt ggcggacagc agccgaaggt cgttgtagct
     2581 cagcgccatg acccgaccct ggatctgcat gaggcggtcc cggaacaccg gatggtcgat
     2641 cagccgctcc ccgccgaggg tctccgtctt catgagctct gtaagggcgt taaatcggga
     2701 gagcatgacg ttcgggtcgc ccagagagtc ccgctcgtgg ccgaggatgg cgttggcgac
     2761 cagccagccc tcaccgcggc ggccgacgat ctgatctttt gggacccgga cgtcggtgaa
     2821 gaacgtctcg ttgaagttcg ccgcgccggt catgtccacc agcgggcgga cctctatgcc
     2881 gggggtgtcc atgcgcagca ggagaaagct gatcccctgg tgcttgggcg catcgggctc
     2941 ggtccggacc aggcagaaca tccagtccgc gatgtgcgcc gtgctggtcc agatcttctg
     3001 cccgttgatc acccactcgt caccgtcgag ctcggcgcgc gtggacaggc tggcgaggtc
     3061 actgccggcc ccgggttccg aatatccctg acaccacagc atctcgccgc gcagcgtggg
     3121 cgggatgaag gtggttttct gctcctgcgt gcccatctcc agcagggtcg gcaccagcat
     3181 ggatatgccc tgaccgccaa gcccggagct cacgcgcgcg gcggcgaact cctcggcgat
     3241 gatccgtgat tcgagaatgt cgggcgcggc gccgtagccc ccgtactcgc gtggaatcgt
     3301 tcttgccgcg tagccgtttt cgatgagcag gtgctgccag gcgagtgttt tctccgaccg
     3361 gatgtgctca ccgcgcgggg cgctcccggc gtggtcggcg agaaacgatc tgacctcctc
     3421 gcggaactct tcgtaatgtg gaccgtagga aagatccata ccaatctccc gggcggaatc
     3481 aggggcgcaa aacagggtgc ggtcgacaac ataaccgcta ccggaagggg cggcaacttg
     3541 gccggtcggg cagggggttc aggccaggcg gtcgagctgt tcccgcctta cccggtactt
     3601 gagcggcacg ccgcgaccgt agcgttcgag ctcctcgatg atgcaatcgg tgagccgctg
     3661 ggtctccgtc cccagcgagc cggcgatgtg tggcgtcagg aagacgttcg gcaacccgta
     3721 caggggtgat tccggcggca gctcctcggg atcggtggtg tccagcacgg cgtagaggcg
     3781 gccgcgcgtg agctctgcct ccagcgccgc ctggtcgatg agagcgccgc gcgcggtgtt
     3841 cacgatggtc gcgccgtcgt gcatcagcgc aagctcgcgt gccccgatca tgtggcgcgt
     3901 ctctttcaac agcggcgcgt gcaggctgac cgtgtccgat tgcgacagca gctcggagag
     3961 ccccaccttg gtcgcgccga ggtctcgcgc ggcaaggggc gtcacgaacg gatcgtagag
     4021 cagcagacgg atgtcgaagc gccgcaggtg ctccatcaca agcctaccaa cgtgagaagc
     4081 gcccacgatg ccgacgctct tgtggtagtt gcctacgtcc ggcgcctcct tcgtccaagg
     4141 tgggcggtgc tcccgatgct cgagatagac gcgattgagc tgaaacaccc gcttgttcgc
     4201 gaagaggatg gcggccaggg tgtactcggc caccggcacc gcattggctg ccaccgcatt
     4261 ggtcaccaga atgccgcggc gccagacgat gtcgtcgaga aagcccttca cgctgccggc
     4321 gagatgggcg atcagcttca ggcgtggaag ccgtgcgagg cagtccgcat cgacccgggg
     4381 gcagccccag ctggtgatca ggagctctac ctggccgagc gcgcccccaa tggcgtccag
     4441 gtcgcggaac ggttcctcgt cgatgaagtt gaccgtcttg cgcaaccgct ccatgtgagc
     4501 gtgcgtgatc agctcgtcgc ggacgacggg atgcataacc agcgcggtgc tcgggctttt
     4561 ggccataggg tgaagaatac cccataattt tctgtctttc gtttacccgc gaggatcggt
     4621 ttgaaaatgg acagagcgcg cgcgctggcg atgatccaga ccatggcgga gatgcaggag
     4681 agccacaatc gccatgtgca cccggaatgg atcgagcagg gttatgccta ttaccgggcc
     4741 gtttgggtgg agtgcgccga gctgctcgac cacttcggat ggaaatggtg gaagaagcag
     4801 cagcccgatc tgggccaggt gaaactcgag gtggtggaca tctggcactt cggcctgagc
     4861 gatctgattc gggcgggcac cctggatacg gcagcgccgg tgctgctgga agtatcggcg
     4921 agcgagccga cggatctcga ggggttccgc ctggcggtgg aggcgctcgc ggccgacacg
     4981 ctttccagcc gtgctttcgc catggcgccg tttgccgcgg tgctcgaaag cctgccgatg
     5041 tccttcgcgg agcttttcga gatgtacgtc ggcaagaacg tgctcaacaa cttccggcag
     5101 gatcacggct acaagaccgg ggagtatcaa aaactctggc agggccggga ggacaacgag
     5161 cacctgatcg acattctggg tgagctggat tgccccgcgg agtcggttcc tgctgagctc
     5221 tatcgagctc tgcaagcccg ctaccccgtc tgagcggcga gctcctcgcg gtaggcgcga
     5281 gccgttcggg cgtcgaagca gacgaaccgg gcaagcaggg gtgtggtggc gatgaagctg
     5341 ccgaccgtgg cgacggcgat ggcggtggct tccgccagcg ggtagccgaa cgcgccggtg
     5401 ctgatggccg ggaaggcgat gctgcggatg tggtggcgcc tggccagatc cagggaattt
     5461 cggtagcagg cggtcagcag cgccgcctcc ccggcgtcac ccccgcgcca gatgggccct
     5521 accgtgtgaa tgacgtgggc ggcgggcaag tcgtagcctc ccgtgattcg tgcttctccg
     5581 gtggggcatc cgccgagcct gcggcattcc gccttcagac cggggccggc ggcccggtga
     5641 atggcaccgt caacgccgcc cccgccgagc agagactcgt tggccgcgtt gacgatcgcg
     5701 tcaacggact gagtggtgat gtcgccttcc acgacctcta tcccggtcat gtcgatgccg
     5761 cccgaagctg tcgcccttcg cccgaagggt agcccccacc cgtccccggg taaaattgcc
     5821 atcttccggc ggcctgtcga caatggcggg ccatgacgct tctggccagc tccatcgcac
     5881 ggctcgacgc ggacgcccgt tgggcgctgc gcggccacga gctcttcttc gcctcgttgg
     5941 cggtcggcga ggcgcagcgg gcggaaggcg ctgccgccga gctgctggct tcggtggagc
     6001 acggtgctcg ggctctgggt gtgggcaaga taggaatcga ccggccgcaa gacgaggcgc
     6061 tcgtcgcctg gtatcagcag cgcggctact acccaggggc ctacccacgg gggtacctcg
     6121 gggagggtcg gggcgaaggc cccgctctcc tagcaaagga tctgaacgct gcccgtgaga
     6181 tggaggtctt ctttcgcggg tgggcgcctg agctcatcgg gaccctgctc ttcgtcatcg
     6241 atggctcgcg ggtgctgctc atcgataaga aaacgggtca cggggcgggc aagatcaacg
     6301 cgccgggcgg caagctggag ccgggagaaa gccctgtggc ctgcgcagtc cgcgaagttc
     6361 gcgaagagat cggcatcacg gttgcagcgc cgcggctcca cgccgaactc gcgttcgccg
     6421 atctgcgcgg cagccagtgg ttcggctacg ttttcgtcgc gcgcgcgtac cggggagagc
     6481 cggtggagac agccgaggcc aggccgagct ggcatccgct gaacgcgatt ccctatggcc
     6541 ggatgtggga ggacgatcgg gtctggttgc cgcgggtggt tgccggcgag cgcgttcgtg
     6601 gccggttcct cttccgcgct ggtcggttgc tcgcccacgc gctggaaggc ctcggtgaag
     6661 tggatggcag cgaagccgag caccggggag gctgccatgg ccgctgatct gaactggctg
     6721 gacagctgcc ggcgcttgag agccattgcc cagacgggac tcgcttacag tcgggatccc
     6781 tacgatcggg atcgatttca ggagctgcgc ctgatcgccg agcggatgct ggcctggctg
     6841 tcggatgcgc accccgatcg cgtcgctgaa gtattcctgc cggaacgcgg ctatcccacg
     6901 ccgaagatcg acgtgcgcgc cggcgtgttc cgcggcgaca gcgtcctcct ggtcaaggag
     6961 cgcgccgacg gccggtgggc cctgcccggg ggatggggcg acgagggcga ttcgcccaga
     7021 tcctccattg agcgcgaggt gctcgaggag tccggctacg aggtcagggc agcacggctg
     7081 gtcgccatca aggaccggca tctgcacccc tacgaaaagc actcgatgct cagggtgtac
     7141 aagctctttt ttctgtgcga tctgctcggg ggagagcctc gcgccagcct ggaaaccgag
     7201 tcggccgagt tcttcccgat cacccggctg ccggagctca gcctcggcag aacgctgccg
     7261 gacgacgttc acctgctcaa cgcctaccgg gcggaaccgg accgcgacgt ttacctggac
     7321 tgagcccatg ggtaatcgct cgtgcgccgg cgcgattgct cctccaaatt agtcgggccg
     7381 gcgcgtctgg tccgttacat cgaccggcac ttctgctgat acgcgaacgg gccgagcgtg
     7441 tcgcctatcg ggtcacggcg tcgacggact gccgcagcac cgagaccatc tcatcgacgt
     7501 gtctttcctc tatgacgaac gggggcccca gacagatgat gtcgcgcatc tcaccggtac
     7561 ccccggcata gaagaagacc ccccgttcca gcgccgcgtc gaccacccgc tgggttatct
     7621 gggcttccat cggaaacggc gtgagggtcg cccggtcgct gacgatctcg atggcctgga
     7681 gcaggccccg accccgcaca tcggacacgt gggggtgctc gcggaaggca tcttgaagcc
     7741 tctccagcag catcgtgccc atgcgatcgg cgcgcgcgac cagttgctcc tcgaccagga
     7801 tgtcgagcac ctcggcagcg gccgcgcacg cggttgggtg ggctgcgaag gtgtgaaaca
     7861 tgacctgcag cccggccgcg ccgatggcgg cgcccaccgg ctccgtggcg aaaacaccgc
     7921 ccagcggagc atacccgccg gcaagcccct ttccggcgat gaggatgtcc ggaagaatct
     7981 cccactgctg gtaggcgaag ttcgttcccg tccggccgaa gcccgtcatc acctcgtcca
     8041 gaatcagcag cacgccgaac tcccggcaga tctcgctcac cctcggccag taatccgcgg
     8101 gaggcacgat ggcgccgccg ctcgcgccgg tgatgggttc cgccaggaat gccgcgacgt
     8161 tttcaggccc tgcctcgacg atgccctcgc gcacggctcc ggcgtagtag gcgcccgcgt
     8221 ccggatgatt ggcgcccagc gggcagcgca gggcgtcggg ggtcgggatc gtcggaggtc
     8281 gaggcagccc gtgttcgagg ccgcgtcttc tcgccgcgtg accgctgacc gcggtagtcg
     8341 cgacggtggt cccgtgatag gacacggcgc gtgacaggat ctgattccgt gacccgtggc
     8401 ctcgggcagc atggtattgc agcgcgagct tcatggcggc ttccacggcc tcggaaccgc
     8461 cggaagtgaa atgcagcctc gtgaaagccg cgtccagcca gttggcttcc agcgcctcca
     8521 ccatggcacg ccgggagggc gttacccagg gaggcaccac gtagctgcag gtcagcgccg
     8581 cctcggcgat gcggtccgct acccgccgtc ggccgtgtcc gatgttcacg acgatcgcgc
     8641 cgccggcggc atcgaggata cgcctgccgt cccgggtcgt cagatggacg ccgctggcac
     8701 tttcgatctc caaggcggcg ccgccaagga acggccagtt gagatcggtc atgggctcac
     8761 tctcgcaagg gtgtttccgc ccggtaggcg gccagatggt ccaccagctg ctgcacgtcc
     8821 cgcgggctta tctggtcggc ccgctccagg gccagctgca acgcggtcac cgcgtccccg
     8881 taagcaccgg tctccgccag cgcggcggcc agggtgtcga gctgccacca gctctcggtg
     8941 tagagcgcca agggacggat cagcgcgacg gcccgggcgc cgtcgcgcag gccgtcctcc
     9001 gtggtggttg cgagcaccct ggcgagggta ttgcgtgccg cgacgctgcc ggcgtccatg
     9061 gccgtctcca ggtaggcgat ggccctggcg gcgtttctgg ggacgatctt gcccgccagg
     9121 taacagtcgg ccagcgcggc gttggcccgg gcgtggccct ggccagcggc tgcggccagc
     9181 agctcgacgg cgcgcggcgg gttgtcttcg atgttgagca aggccagccg atagcgcgcc
     9241 tcggcgacgc ccgccgccgc tgcgagggcg taccactggg ccgcccgggc cgcgtcggcc
     9301 gacgtgccgc ccagatcgtc gaacgcgcgg ccgaggtaga gctgagcata ggggtcaccg
     9361 cgcgacgcct gggtgagcag ccattcctgg agcggcgcgc tgacgccgcg gttgcggtcc
     9421 ttgaaaagcg gaccggcgta ctccagcccg gtgggcagct ttacgagccc ggtccgcacc
     9481 cggctgccgg tccacatgcc ggcaatctcc acgccggcga cgtcgcgacg cgtacccggg
     9541 ccaaggggct ggttgttctc ccaggccccg tcgtggaagc ctccttcggg gtccgtctcg
     9601 cggccctgac cctgccgctt gccgtgatgc cactcgcctt cgtagctgct gccgtccggg
     9661 cgcagaagaa tgccgaagcc gtgataattg cccgcgttcc acgtgccctc gtaggccata
     9721 tccagggcgt tccgcgaagc tccatagccg tcgcggcttc cggccgtcca tcggccgtcg
     9781 tattcggatc cgtcttgctc ggtgagaatg ccgaagccgt ggggcacgtc ggcgagccat
     9841 tccccttcgt agcggttgcc gctcgcgtcg gtgtacgtcc cggtgccagc acgctcgccg
     9901 tcgagccact ggccctggta gaggcttcca tcgttggcat tgaagcggcc ctcgccgttc
     9961 gggagatcgc ccgcccattc gccccggtag aagccgtcct gggatttttg ggttcctgcg
    10021 ccttctcgca ggccgttgac aaagctgccc acgtaatgat cgccgttcgg gttcgtcagc
    10081 tcgccgtggc cgtggcgtct gccctcgttc cacatgccgc tgtagcgctc accgtcgtcg
    10141 ctgcgcatga gcccggtgcc gtgtggcctg ccggacagcc agccgcccgc ataggcgcgc
    10201 ccgtcgggcc attcgtagcg agcctgcccg gtgaatgcca gatcgttcgg gtagacccac
    10261 acggtgcgcc cggagggcag cgtgagcctg ccggcagcgg gatcgcctcg gggggcggtg
    10321 ggaccgtcta tggagaccgt agagcatgcc gccaggagca gacctgcggc agcgaagagc
    10381 agatgtcgcg tcactgggtt atggaaaacg atctcatgtc aaaactcaaa ggctttgggt
    10441 aaccttgccc gggtctggaa cgggagaatt ccgtgaccgc gagcaaagac gacggcagca
    10501 tcgaaggcgt cgacatcccg cgcgtgaccc attggctggc aggccggatc gccggcctgc
    10561 ggccgccgct ggagttttcg ctgattgcgg gcggtcactc gaacctcacc taccgctttg
    10621 ccgatcaggc tgacgccgag tacgtgctgc gtcgcccgcc cctgggccac gttcttgaga
    10681 gtgctcacga catggctcgg gagcaccgta tcgtctccgc gctcgcgggc accgaggttc
    10741 ccgtggcgcc cacccatggc ctttgtgagg accgggacgt caacggggcg cccttctacg
    10801 tgatgagcta cgtccggggg gccgtgctgc acgatgcgga cgccgcccgt gagctcgatc
    10861 cggccgagcg gcatgccctt ggcgaggatg tgatcaacgt gctcgccagg ctccatgccc
    10921 tggatcccga cgcagtgggt ctcggcacgc tggggcgcaa ggaagcctac cttgcccggc
    10981 agctcaagcg ctggaagaag cagtgggagg catcgaagac ccacgagatt cctgagatgg
    11041 aggagacggc caggctgctc gaggaaaaca tgcccgagca gatcggggcg gccatcgtcc
    11101 acggtgacta tcgcctgggc aacatgatcg tcgccgacgg ccgtattctt gccgtcctcg
    11161 actgggagct ctgcacgctg ggcgacccgc tggcggacgt cggctacctt ctgaacaact
    11221 ggattcagcc cgaggacggc gcgggtgagc tcgtgccgac ggcggccggg gggttcccgt
    11281 cccgggaggc cctctgtgac ggttacacgg cggcgacggg gcgggacctc tcggggatca
    11341 actactaccg ggctttttcc cactggcgtc tggccgccat cggtcagggc gtctacaagc
    11401 gatacctggt gggcgccatg ggcgagcgcc cggacatgga cctggacacc tacaaggcga
    11461 gcgtccagca gcgggcggcc gccgcgctcg agctgctgac ggcttagccg ccgggcagca
    11521 cgatctcgcc cagcggggtg tcgtcctggt cgatgacgcg cagccggctg aagccctcac
    11581 cttccagccc gatgacgatg cagggattgg tgatcacctg agcgagcacg gcatccgcag
    11641 gcggctggtt ccaggccagc cgtaccgtga ccgcatcgcc tttcatggtc cccgcgacga
    11701 gctcgaagcc gtaacccggc gtgggctgct ggcctctgga gaccgcgacg agcagcaggt
    11761 ccgtgggcgt ggtggcgggc tcgtcagggg cagacagcag cctgcccccg cggatgcgcg
    11821 ccacatcctc gatgctcacc acttccgttc cggcctggga catcctgcat tggtcgtggt
    11881 tgagcacttc caccaaagag acgtcgtcac gaccgctgca gccgtaaagc gcggcgacga
    11941 gcaacgcacc tagaattgtc cttgagcggg tcatctggga gctcccccat ctgccttgcc
    12001 cggcaagcgt acggcttcgg gtgcgacttg cccagagacg tcggcgttcg ccgagaggtt
    12061 ggtcaggttg acgcgaaact cggaggtgaa gtcgatgccg gtcaggtctt cgccgtccac
    12121 cgtgacgacg gccggagagt ccagagtccc gtaggtgccg cacgcttctc ccgcgtcgca
    12181 cagaaaggcg tcgtcgtcgg agtccgtccc ggcgaatatc cggtagtcgc cgggcgcgac
    12241 gccgtcgagg cggtaggcgt actccccgtt cgtggcctcc aggacgttct gaggtgacgg
    12301 aagggtctcc cccgccgcat cgacgagcac gacgtagtgc acgccggcgt tggcgtccgg
    12361 gctggtggtg aaaacccgca tcgttgcggt gacggtggcc gtggggttca cggcatcggt
    12421 cacgatttcg atggcgccgg tgtaggagcc cggggtaagg cccgtgcggt ccacggtgac
    12481 cgtgtaggaa cccatgccgc cgggcagcac cgagcttggc gccacgtcca gccaggatgc
    12541 ggctgtgccg atgctctgca ccgtcaccgt gccggagccg acgttttcca gtcgaagaga
    12601 ctgtgacgac agtgccgccc cgaaggtcag ggaactggct gacacggcca gggtcgggcc
    12661 aggcgggttg ctggccaggt ccagggcggc cagcaccgcc ttctgggcgt tgatcaatcc
    12721 ccagccgtag cggtcgtccc gccccggcgc gcccagatcg tcggtaatcc ggcccgacgc
    12781 gagcgcgttg tcgaactgga tgggggtcag gccggggtga acggccttca tcagagcaac
    12841 gacgccggca acgtgaggcg cggccatgga cgtcccctgc agggatgcgt agccgaagcg
    12901 caggctcgag gggtcgctgt cgtcggccat ggtgctgatg accccgtctc cgatcccgtc
    12961 gccgttgacg tcggtgacgt tggacccgcc cggtgccgcc acgtcgacgg tggtcccgaa
    13021 gttcgaatac gccgccggat tgttctcgat ggtggtggcg ctgacggaga cgaccccgtc
    13081 gtaagcggct ggatagctgg gcgaggcgga cgcatcgttg ccggcgctgg caacgatgat
    13141 ggtgcccgcc gcgcggatct cgtcgatggc ctgctgctcg ctttcgagaa acccgccccc
    13201 tccgagactc aggttgatca cgtccgcggc gcggtctgga agggtgccgg agacgttgtc
    13261 gaggcgggcg gcccatcgca tggactcgat gacgtcgaag ctggtgccgc cgcccacacc
    13321 gagaacccgc aggggcatga tcttcgcctc ccacgcgacg ccggcaacgc ccgaggccgg
    13381 ggtgaccgag gcggatgttt ccgccgccac ggttcccgca acgtgggtgc cgtgaaagct
    13441 gctggaaccg ccgaacgccc cgtcgcccgg atcggtcgga tcgtcgtcgc gagcatcccc
    13501 gtcggctgcc cgctgcagat tggcgatgaa gtcgaagccg gcgatgacct tctcgtcgag
    13561 gtcggggtga tccgcccgga tgcccgtgtc gaccaccgcg acgatcacgt ccccatcgcc
    13621 cgtggtgatg tcccacgcca gaggcaggtt gatggcctgg tagtgccact gggaccggta
    13681 gaaagagtcg cccggctccc ggtgtggctt ccgaatcagg ttgggttccg caagctcgac
    13741 gcggggatcg cggcccagcg cctcgatgac ggccagggtg gcgtatcgat cctgctgatc
    13801 ttcggtccac gaggcatcga atggtgcccg ccgatcgatg tgccggagcg cggccgttgc
    13861 cgggccggct ttcagtagca tccgcgggcc ggactgagcg agcatgctga ggccgtactc
    13921 cgccgcgatc tcatcatcgg tcgtgcgcag gatgatctct cgtggcagga acggatcgat
    13981 catggtggtg gcccgctcgg ggacgaggtc ctgacccacc gtgagcacgt agttgctggc
    14041 gccggcgatg gggcgaacct cgatgaagta gtctccggcg gcgggaacct cggcgacctg
    14101 aacctctccc ggcccgccgt cggcgctgat cacgttctgc tgcgagtcgt aaaggagcag
    14161 ctcctgctcc gcgttctcgg gatcggcgat gttcaggtag atgaactcgt tgccgaccat
    14221 ggtcatggcg tagaaatcgc cgggatcgcc gtcctggaag agctttcccc tttccggctc
    14281 gtcgcccacg ccgacgcccg ctacgttggc gaaccccccg agggtcaccg gattaggcaa
    14341 aggctgcgcg ctggcaaagt cgttgttcgg cgccggcgtg ctgcctacat cgttcacgtc
    14401 gctgtcgatg gcgctggagg ccagaattcg tacggtaccg ctgatgcttg cgacaggccc
    14461 ctccaccgtg acggtgcgag tcgtgctccc ggtggcgccg tcgtcatcgg taaccgtaag
    14521 ttctacggtg taggtgccgg cctcctcgta ggtatgggac gtgttaacgc cgctggtgct
    14581 cgatgctcct ggaggctcgc cgaaggtcca tgcgtagctt gcgatgcgtc cgtccgcatc
    14641 gctggacccg ctggcatcga aatcgacgct taaaggcacg gttccctcgg tcggggtggc
    14701 cgtgaaggat gccgccggcg accggttagc gggggccggg gtgggggtgt cgctgccgcc
    14761 cccgcccccg ccacaggcag tcagggttaa tacgcaggta accgtccaca cggtttttgg
    14821 caatgcttgg cgaatcatct ttgcccactt ctgcccatca cggccgggtc cgccgatttt
    14881 tgcacagatc gggtgccggc tctgtgcgcc gggcagcacg atggcgttgc ccgggcggtc
    14941 aacgttgcga aaaggctggg gcgttgcgaa aaggctgggg ccggaaacga cacaggcctc
    15001 tgaaagaggc ctgtgcgtta ccgtgggtct gatccggccg gctgccggct cagtccgtgt
    15061 aaaaaaggcg tgtcagcagt gggcaccgct gaaacaactt ctcttctggg ggaaggacac
    15121 ttgaaaggga ggactgcat
//
LOCUS       DBNHKV010000266         1643 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1878795, whole genome shotgun sequence.
ACCESSION   DBNHKV010000266 DBNHKV010000000
VERSION     DBNHKV010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1878795"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="AC16PP_08425"
     CDS             <1..579
                     /locus_tag="AC16PP_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIR6481691.1"
                     /translation="LTLAFSAVVATAAMIADAPYLGFLPTLVGFFGLLFIVHKTANSP
                     WGLFWTFAFTGFLGYGLGPILNAYLTLANGDVLIASALGTTALAFVGLSAFALVTRKD
                     FSFLSGFLVVGFFVLMGAVLLAWLFDLSGFHLAISCGFVLFACAAILWQTSAIIHGGE
                     TNYILATITLYVQIYNLFISLLHIFGAFGSDD"
     gene            complement(599..>1643)
                     /locus_tag="AC16PP_08430"
     CDS             complement(599..>1643)
                     /locus_tag="AC16PP_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HIR6481692.1"
                     /translation="ERATIRDAVGRPRPDARSYRYDEWDYLNRSYRTRWCRLFEERLA
                     PDTQQDLDALRHVIRRHRRAVQSQLEHIKPQGYQRVTRVADGDELDFNAVIEARGDIR
                     AGLTPDERVYSRRERVHRDVCAAFLVDLSASTDDPVDPPEAVAAGAVEELANLRDPYE
                     FPVAHDEPAEEPRRIIDVQRESMLVMSAALEQLADSYGIYGFSGYGRECVEFFVAKEP
                     DEPFTRGTLSAIAGMKPKRSTRMGPAIRHATSKLLRSGHALKVLIILSDGFPQDSDYG
                     PERGDHEYGVQDTAKALREAEEKGVETFCVTVDRSGHDYLKRMCPNARYMVIEETRDL
                     PGALSKVYEALTG"
BASE COUNT          230 a          557 c          518 g          338 t
ORIGIN      
        1 ctcacgctgg cgttcagcgc ggtggtcgcc accgcggcga tgatcgcaga tgccccctac
       61 ctcggttttc tcccaaccct ggtcggcttc ttcggtcttc tgttcatcgt gcacaagacg
      121 gccaatagcc cctggggcct cttctggacc ttcgccttca cgggctttct gggctacggg
      181 ctcggtccga ttctcaacgc gtatctgacg ctcgccaacg gtgacgtcct gatcgcctcg
      241 gctctgggca ccacggcgct cgccttcgtc gggctctccg cgttcgcgct ggtcacccgc
      301 aaggacttca gctttttgag cggcttcctc gtggtcggct tcttcgttct gatgggcgcg
      361 gtgctgctgg cctggctttt tgacctctcc ggcttccacc tggccatatc ctgcggcttc
      421 gtgctgtttg cctgcgcggc cattctctgg cagacgagcg ccatcatcca cggcggcgag
      481 accaactaca tactcgccac catcaccctc tacgtgcaga tctacaacct gttcatctcg
      541 ttgctgcaca ttttcggagc gttcggcagc gacgactgaa cgccgcgaca gatcctgatc
      601 agccggtgag cgcttcgtag accttgctga gcgctcccgg cagatcccgg gtttcctcga
      661 tgaccatgta gcgcgcgtta gggcacatgc gcttgaggta gtcgtgcccc gagcggtcca
      721 ccgtgacgca gaacgtctcc acgccctttt cctcggcctc ccggagcgct ttggcggtgt
      781 cctgcacgcc gtattcatga tcgccccgct ccggcccgta gtcggagtcc tggggaaagc
      841 cgtcgctcag gatgatcagc accttcaggg cgtgacctga gcgcagcagc ttggacgttg
      901 cgtggcgtat ggccgggccc atgcgggtgc tgcgcttggg cttcatcccg gcaatggcgg
      961 acagggtgcc acgcgtgaac ggctcgtccg gctccttggc gacgaagaac tcgacgcact
     1021 cgcggccata tccggaaaag ccgtagatgc cgtagctgtc tgcgagctgc tccagggcgg
     1081 ccgacatgac gagcatggat tccctctgga cgtcgatgat gcgacggggc tcctcggccg
     1141 gctcgtcgtg ggcgaccgga aattcgtagg gatcgcgcag gttggcgagc tcctcgaccg
     1201 cgccggccgc gacggcttcg ggcggatcca ccggatcgtc cgtggacgcg gacagatcga
     1261 cgagaaaggc agcgcagacg tcccggtgca cgcgttcccg gcggctgtag acgcgctcgt
     1321 ccggggtaag ccctgcgcgg atgtcgccgc gggcctcgat caccgcgttg aagtccagct
     1381 cgtcaccgtc cgccacccgg gtgacccgct gatatccctg gggcttgatg tgctccagct
     1441 ggctctgcac tgccctgcgg tggcggcgga tcacatgccg cagcgcgtcg aggtcctgct
     1501 gtgtgtccgg ggcgaggcgc tcctcgaaca gccggcacca tcgcgtgcgg tacgagcggt
     1561 tcagatagtc ccactcgtcg tagcggtagc tgcgagcatc cggtcgaggc ctgcccacgg
     1621 cgtcgcgaat cgttgcccgc tcc
//
LOCUS       DBNHKV010000267         3183 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1526161, whole genome shotgun sequence.
ACCESSION   DBNHKV010000267 DBNHKV010000000
VERSION     DBNHKV010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1526161"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="AC16PP_08435"
     CDS             complement(<1..633)
                     /locus_tag="AC16PP_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6481693.1"
                     /translation="MQRLKNSTLFYYSLTDLPIAMSMFPVAVFIPRFYASDVGVPLAV
                     VGTIILVVRIFDVVTDPLMGYLSDQTRTRFGRRKPWIFAATPIMMTSIYMLFMPPEDA
                     GWLHMFTWSMLLSIAITMMLIPYYAWGAELSPDYNERSRITGGRAMAGVFGSLSAQLI
                     PAGALLFFGLGGSDVVLTLVGTTMLVLMPICVLLTLLNTPEPEVRHKSTVP"
     gene            819..1232
                     /locus_tag="AC16PP_08440"
     CDS             819..1232
                     /locus_tag="AC16PP_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6481694.1"
                     /translation="METAVKLAIHDLLGRSAYALDERHVDMLEGTFTEDAAFEIDIQG
                     VDGTMSFAGREAIMGLMTDSMAEQTDQRRHVVTNVFFEEVGEERARLISNITITSVEH
                     GQIRLVTSGLYRDEVVLHNGDWRIARRRIELDMPY"
     gene            1248..2027
                     /locus_tag="AC16PP_08445"
     CDS             1248..2027
                     /locus_tag="AC16PP_08445"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6481695.1"
                     /translation="MNLKGQVAVISGAAVGIGRGIAVALAEQGAAVACLDIDGVGNQE
                     TAGLAEEAGGRGLALDCDVADRAQVRRAMSEALGALGRIDVLVNNAAIYVDTSLTVGS
                     YDVQTRAYERSIAICALGSYYCARAAVPAMRESGGGNIVNIITEHIKEGHLMTGPGAT
                     GYDGAKWVMWRQTESWAVELAEHNIRVNALCMGATDTPMLRAVSVPIAEAGMRPEDVG
                     QAVLNVLAHGRRGPTGQSYVFGTTGTSREQSLGEIAALAPR"
     gene            2045..>3183
                     /locus_tag="AC16PP_08450"
     CDS             2045..>3183
                     /locus_tag="AC16PP_08450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6481696.1"
                     /translation="MRDRFSQAYLWYVVAVLTIASTFSIIDRQILNVMIGPVKRDLGG
                     ISDFQVSLIMGFAFTFLYSVLSYPAGWVADRYNRKNLMAVGIAAWSAMTMLCGMVSQY
                     WHLFATRMGVGVGEATLQPAATSSLSDYFPPHMLPLAMGIVASAPFIGQGLANIAGGP
                     LIDYLEATPNYVLPLFGEVFSWQMVFLLVGAPGLLVALAMWGLREPSRHGKQTEEAGV
                     PLREVWAFVKDRGAFFFLVFTAYLCLATQGWSLFSWLVEFYVRNHEWTRTEIGLTYGF
                     IAMFVGMAGSIIGGAWAGRLIRRDSPDGTIRVVMYGSIVLLPVGAAITIVPDPWLGVA
                     LLVPATFCMAMPPGLIMATLQAIAPNELRGQMVAFYLIAVNFLAYT"
BASE COUNT          535 a          998 c         1073 g          577 t
ORIGIN      
        1 cggtaccgtt gacttgtggc gcacctcggg ctccggggta ttgagcaggg tgaggagcac
       61 gcaaatgggc atcagcacga gcatggtcgt gcccaccagc gtcagtacca cgtcgctgcc
      121 gcccaggccg aagaagagca gggcgcccgc ggggatcagc tgcgcgctca ggctgccgaa
      181 cacgccggcc atggcccgtc cgcccgttat cctcgagcgc tcgttgtagt ctggtgagag
      241 ctccgcgccc cacgcgtagt aggggatcag catcatcgtg atcgcgatgg acagcagcat
      301 gctccaggtg aacatgtgca gccagcccgc gtcttcaggg ggcatgaaca gcatgtagat
      361 cgaggtcatc atgatgggcg tcgcagcgaa gatccagggc ttgcgccggc cgaaccgggt
      421 gcgggtttga tcgctgaggt agcccatcag agggtcggtg accacgtcga agatccgtac
      481 caccaggatg atcgtgccga ccacggccag gggaacgccg acgtcgctgg cgtaaaaacg
      541 cgggatgaac acggccacgg ggaacatcga catggcaatg ggcaggtcgg taaggctgta
      601 gtaaaacagc gtggagtttt tcagccgctg cacgtggatt tccttccggg gcggcctatt
      661 ggacttggcg gcgcatttgc gtgtcaagga agctcgcaac ggtggcgccc gggccgatca
      721 tcgcctcaac attcctggcg ctgctgcggc tggcaatcct gcaaggaatg ccataacgtc
      781 ggggctgcat cgacggccgg atccgctggg aggggaagat ggaaaccgca gtcaagctcg
      841 ccattcacga tctgttgggg cgctccgctt acgccctgga cgaacgtcac gtggatatgc
      901 tggaaggaac gtttacggaa gacgctgcct tcgagatcga tatccagggc gtcgacggca
      961 cgatgtcctt cgcgggtcgc gaggccatca tggggctgat gaccgattcc atggcggagc
     1021 agaccgatca gcgacgccac gtggtcacga acgtgttttt cgaggaggtg ggagaggagc
     1081 gcgccaggct gatctcgaac atcaccatca cttcggtgga gcacggccag atccgtctgg
     1141 tcacctccgg cctgtatcgg gacgaggtgg tgctgcacaa cggtgattgg cgcatcgccc
     1201 ggcggcggat cgagctggac atgccttact gatcccaagg aggctgcgtg aatctcaaag
     1261 gtcaggttgc cgtcatcagt ggtgcggccg tgggaatcgg gcgcggcatc gccgtggctc
     1321 tggcggaaca gggcgcggcg gtcgcttgcc tggacatcga cggcgtggga aaccaggaga
     1381 cggccgggct cgcggaggag gcgggtgggc gcgggctcgc gctcgactgt gatgttgccg
     1441 atcgagccca ggtgcggcgt gccatgagcg aggcgctggg cgcgctggga agaatcgacg
     1501 tgctggtgaa caacgccgcc atctacgtgg acacctctct cacggtgggc agctacgatg
     1561 tccagacccg cgcgtacgaa cgatccatcg ccatctgcgc cctgggcagc tactactgcg
     1621 cccgggccgc cgtgcccgcc atgcgcgagt ccggcggcgg caacatcgtc aacatcatca
     1681 ccgagcacat caaggaaggg cacctcatga ccggaccggg cgccacaggt tacgacggtg
     1741 cgaagtgggt aatgtggcgg cagaccgaat cctgggccgt ggagctcgcc gagcacaaca
     1801 tacgggtcaa cgcgctctgc atgggcgcta ccgacacgcc gatgctgcgg gcggtttcgg
     1861 tgcccatcgc cgaggccggc atgcggccgg aggacgtggg ccaggcggtt ctgaacgtgc
     1921 ttgcccacgg tcggcgcgga ccgacggggc aatcctacgt cttcggcacc acgggcacgt
     1981 cccgggagca gagtctcggc gagatcgccg ctctggctcc gcgctgagcc cgccccgggc
     2041 acccatgcgg gacaggttca gccaggcata cctttggtac gtggtggcgg tgctgaccat
     2101 cgcctccacc ttctccatca tcgatcgtca gatcctgaac gtgatgatcg gacccgtgaa
     2161 gcgcgacctg ggcggcatct cggatttcca ggtctcgctc atcatgggct tcgccttcac
     2221 cttcctctac agcgtgcttt cctatccggc cggctgggtt gccgaccgct acaaccggaa
     2281 gaacctgatg gccgtgggca ttgccgcctg gagcgcgatg accatgcttt gcggcatggt
     2341 gagccagtac tggcacctct tcgccacgcg catgggggtc ggggtcggcg aggcgactct
     2401 gcaaccggcg gccacgtcct cgctttccga ctactttcca cctcacatgc tgccgctcgc
     2461 catgggcatc gtggcctcgg cgccattcat cgggcagggc ctggccaaca tcgccggcgg
     2521 tccgcttatc gactacctgg aggcaacacc caactacgtg ctgccgctct ttggtgaggt
     2581 attttcctgg cagatggtgt tcctcctggt tggcgcgccc gggctgctgg tggcgctcgc
     2641 catgtggggg ctgcgcgagc cgtcccgaca cggcaagcag actgaagagg ccggcgtgcc
     2701 gctccgcgag gtctgggcct tcgtcaagga tcggggggct ttcttcttcc tggtcttcac
     2761 cgcctacctg tgcctggcta cccagggctg gtcacttttc tcctggctcg tggagttcta
     2821 cgtgcgcaac cacgagtgga cgcgcacgga gatcggactg acctacgggt tcatcgccat
     2881 gttcgtcggc atggcgggca gcatcatcgg cggggcctgg gcggggcggc tgatccgccg
     2941 cgactcgccg gacggcacga tccgggtcgt catgtatgga tccatcgtgc tcctgccagt
     3001 cggggccgcc atcaccatcg tccccgaccc ctggctgggc gtggctctgc tggtgcccgc
     3061 cacgttctgc atggccatgc cgccgggcct catcatggcg accctgcagg ccatcgctcc
     3121 caacgagctg cgcggccaga tggtggcctt ctatctcatc gcggtgaact ttctcgcgta
     3181 cac
//
LOCUS       DBNHKV010000268         3031 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_178884, whole genome shotgun sequence.
ACCESSION   DBNHKV010000268 DBNHKV010000000
VERSION     DBNHKV010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_178884"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="AC16PP_08455"
     CDS             <1..760
                     /locus_tag="AC16PP_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR6481697.1"
                     /translation="QCQGPAKRRYAAFAAGCGITPVLALVRAKLAEEPGCSWTVFYGN
                     RSAGRAMFLDELLGLKDRYLGRLALHFVMSREPSEAEMFNGRIDAAKVQASAGKLFDP
                     AATDEFFLCGPGDMIAAVGAALGKLGVAPDRIHSEHFTVESVAAADRDGEPPPRTGGA
                     AEIIVIMDGRRRSFTMSPDDESILDAALDSGIDLPYSCCAGVCSTCRTRVVEGAAEMA
                     ENFSLEDWEIENGYVLACQSRPTSSELVLDYDDV"
     gene            806..1675
                     /locus_tag="AC16PP_08460"
     CDS             806..1675
                     /locus_tag="AC16PP_08460"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6481698.1"
                     /translation="MGNVGFVGLGVMGYPMAGHLARAGYNVRVYNRTGAKSERWVTEH
                     GGSRVETPREAASGADVVCLCVGNDDDVRAVVYGPEGVLDGLADGSVLIDHTTVSAEL
                     AEELASAAGERGCGFLDAPVSGGQAGAENGQLTVMVGGEQTIFDSALPVMEAYGKSVR
                     RMGPAGTGQLTKMVNQICIAGVLQGLAEGLHFADQAGLDIESVVDVISKGAAQSWQME
                     NRHATMARGEFDFGFAVDWMRKDLAIALQAGRRLQARLPLTALVDQFYADVQSYGGGR
                     WDTSSLIQRLRRT"
     gene            1802..2224
                     /locus_tag="AC16PP_08465"
     CDS             1802..2224
                     /locus_tag="AC16PP_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013319.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR6481699.1"
                     /translation="MSDDRAIASWSHFDHAADMGVRGVFARCFPAARLEMLFDVSHNM
                     CKVETHRVGDETRELFVHRKGVTRAFGPGHPSLPDPLRSVGQPVLIGGSMGTESYVLR
                     GTKESERRAYSSACHGAGRAMSRRAATRRWRGWFPCSA"
     gene            2330..>3031
                     /locus_tag="AC16PP_08470"
     CDS             2330..>3031
                     /locus_tag="AC16PP_08470"
                     /inference="COORDINATES: protein motif:HMM:NF015644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HIR6481700.1"
                     /translation="MYVLLFVALLGCGAWLAHSGSRHHRKVQVWGGAAIVGSAFGLML
                     LMAFWAEMLWFDSLGYQDRFWTFVWVRLLVPLLGGAAAGLTGLVLTRGAGIYLRRGVA
                     FACAAAGVIWGLASWQPVLLFLNRMETGIVDPLLGLDTGFYLFVLPLLDGVFWLGLFV
                     ALISLITVLALREQDGVVMPGPAFAVPKGRLLIPGASLTLTVVLAFGQLLSVLHLLYS
                     KLGVVMGPGWTDVHVR"
BASE COUNT          439 a          935 c         1079 g          578 t
ORIGIN      
        1 tcaatgccag ggaccggcga agcgccgcta cgcggctttc gcggccgggt gtggaatcac
       61 cccggtgctc gcgctcgtgc gcgccaagct cgccgaagag ccgggctgca gctggacggt
      121 gttctacggc aaccgcagcg cgggccgggc catgtttctc gacgagttgc tgggcctcaa
      181 ggatcgctac ctcggccggc tcgccctgca cttcgtgatg agccgtgagc cgagcgaggc
      241 cgagatgttc aatggccgca tcgacgcggc caaggtccaa gcaagtgccg gcaagctctt
      301 cgaccccgcc gcgacggacg agtttttcct gtgcggcccc ggcgacatga tcgcggcggt
      361 cggcgccgcc ctgggcaagc tgggcgttgc cccggaccgg attcacagcg agcacttcac
      421 ggtggaaagc gtggctgcgg cggatcgcga cggggagccg cctccacgga cgggtggcgc
      481 ggcggagatc atcgtgatca tggacggccg tcgccgatcg ttcaccatga gcccggacga
      541 cgagtccatc ctggatgccg cgctggattc cgggatcgat ctgccttact cctgttgcgc
      601 cggcgtgtgc tccacgtgtc gtacccgcgt cgtggaaggt gcggcggaga tggctgagaa
      661 tttttcgctg gaagactggg aaatcgagaa cgggtacgtg ctggcctgtc agtcccgccc
      721 gaccagcagc gagctggtgc tcgactacga tgacgtgtag gcagccagtg tggctccaag
      781 ccagaggagg tggatggtga gcgggatcgg caatgtgggc ttcgttggcc tcggcgtcat
      841 gggttatccc atggccggac acctggcccg cgcgggctac aacgtgcgcg tgtacaaccg
      901 aaccggcgcc aagtccgagc gatgggtaac cgagcatggc gggtcccggg tcgaaacccc
      961 gcgggaggct gcgtcggggg cggacgtggt ctgcctttgt gtgggcaacg acgacgacgt
     1021 gcgcgccgtc gtctacgggc ccgagggtgt gctcgacggg ctggccgacg gctcggtgct
     1081 gatcgaccac accacggtct ccgcggagct ggccgaggag ctggcttctg ccgccggcga
     1141 gcgcggttgc gggtttctgg acgcgcccgt ctccggaggt caggcgggag ccgagaacgg
     1201 acagctgacg gtgatggtgg gcggggagca gacgatcttc gacagcgctc tgcccgtcat
     1261 ggaagcctac ggcaaatcgg tccgtcgcat gggtcccgct ggcaccgggc agctcaccaa
     1321 gatggtcaac cagatctgca ttgccggggt tctgcagggg ctggcggagg ggctgcactt
     1381 tgccgatcag gccgggctgg acatcgaatc ggtggtggac gtgatctcca agggcgccgc
     1441 ccagtcctgg cagatggaga accggcacgc caccatggcc cggggcgagt tcgatttcgg
     1501 tttcgccgtt gactggatgc gcaaggatct cgccatcgcg cttcaggccg gacgtcggct
     1561 gcaggcccgt ttgccgctca cggcactcgt ggaccagttt tatgccgacg tgcagagcta
     1621 cggtggcggc cgttgggata cttccagcct gatccagcgt ttgcggcgca cctgaccacc
     1681 ttggatcgcc acctcggaca tcccctatgt tcgagctttc ggctacgcgg tagcgtcgga
     1741 aatcgtcgcc gatcacggca tttgttgcgc gcggcaaccg tgcgaatcat ggggctgaca
     1801 gttgagcgac gatcgggcca tcgcgagttg gagccacttc gatcatgcgg ccgacatggg
     1861 ggttcgcggg gtgttcgcca gatgcttccc ggcagcgcgg ctcgagatgc tcttcgacgt
     1921 ctctcacaac atgtgcaagg tggaaaccca ccgggttggc gacgagaccc gggagctgtt
     1981 cgttcaccgc aagggtgtca cgcgagcgtt tggccccggt cacccgagcc tgcccgaccc
     2041 cctgaggtcc gtggggcagc cggtgctgat cggcggcagc atgggaaccg agtcttacgt
     2101 tctgcgcggg acgaaggaaa gcgagcgccg cgcctactcc tccgcctgtc atggggcggg
     2161 tcgggccatg agccgccgcg cggccacccg gcgctggcgc ggctggttcc cgtgctctgc
     2221 gtgaagggct aacggcgccc gacacggcct taaggtctag gtagtaacca cggttgccca
     2281 ggcgaacccg cctgccagac tggaacggtc ggcaagaagg gtgtgaacca tgtacgtcct
     2341 gcttttcgtt gctctgctgg gctgtggagc ctggctggct cattccggca gtcggcatca
     2401 ccgcaaagtg caggtctggg gcggtgcagc catcgtcggt tccgcgttcg ggctgatgtt
     2461 actgatggct ttctgggccg agatgctgtg gttcgactcg ctcggctatc aggatcggtt
     2521 ctggaccttc gtctgggttc gccttttggt gcctctgctg ggcggcgccg cggcgggcct
     2581 cacaggactg gtgctgacgc ggggcgcggg catctacctc agacgcggcg tggccttcgc
     2641 ttgcgccgcg gccggcgtga tctgggggct tgcatcctgg cagcccgtgc tgctctttct
     2701 caatcgaatg gaaaccggga tcgtcgaccc gctgctcggg ctggacacgg ggttctacct
     2761 tttcgttctg cccctgcttg acggcgtctt ctggctgggt ctcttcgtcg ctctgatctc
     2821 tctcatcacg gtcctggcgt tgcgcgagca ggacggggtg gtgatgccgg ggccggcgtt
     2881 cgcggtgccg aaggggcggc tcctcattcc cggcgccagc ctgacgctga ccgtcgtgct
     2941 ggcctttggc cagctcttga gcgttctgca tctgctgtac tcgaagctcg gcgtggtgat
     3001 gggcccgggc tggaccgacg ttcacgttcg g
//
LOCUS       DBNHKV010000269         3002 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1313173, whole genome shotgun sequence.
ACCESSION   DBNHKV010000269 DBNHKV010000000
VERSION     DBNHKV010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1313173"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="AC16PP_08475"
     CDS             <1..1265
                     /locus_tag="AC16PP_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003379714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HIR6481701.1"
                     /translation="AIMVIDGSKGVEPRTIKLMEVCRLRDTPILTFINKLDREIRDPV
                     EVMDEIEDILQIQCAPMTWPIGMGRQFKGIYDLYADEIVCFVQGHGHHIFDYPRIKGL
                     ESNAARELLGSDHDDVVEEIELVRGASHAFDVDDYLGATLSPVYFGTALGNFGVKEML
                     DGFVTYAPPPQARHADARDVKPGEEQFSGFVFKIQANMDPKHRDRIAFLRVCSGRYAQ
                     GMRMRHVRLGKDVKVADAVTFLAGDRAQTVEAFPGDIIGLHNHGTIQIGDSFTQGEVI
                     KFTGIPNFAPELFRRVRVKDPLRSKQLERGLDELSEEGASQVFKPLIRNELIVGAVGM
                     LQFDVVAFRLREEYGADCVYDSTNIFAARWVVSDDKKKLEDFTRKHRDQLAVDGGGHL
                     AYLAPTRANLQLVEERWPEIRFLKTREH"
     gene            1266..2057
                     /locus_tag="AC16PP_08480"
     CDS             1266..2057
                     /locus_tag="AC16PP_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024762.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HIR6481702.1"
                     /translation="MAGLAEQELIQAVRAFASDPEADELEAAVLVNRALEPSLDADTL
                     RGRIAELAAACPETQTPWDYLRELGFVGNAANYDALDNSRLDRLLDTGRGIPIALGVL
                     LIEVARASGRMAVGVNFPGHFLVRVGESLVDPFRMTQTSEDECVAGLGQARGNPGAGG
                     DLFPSATAAAVLLRMLNNIKYRYAQAGEWHRSLDMVDLQLAAAGESAHLLLERGELWL
                     RLGARGPARADLERALALAGDGDPRLAAAARRLLDGLGSGPELLH"
     gene            complement(2075..>3002)
                     /locus_tag="AC16PP_08485"
     CDS             complement(2075..>3002)
                     /locus_tag="AC16PP_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HIR6481703.1"
                     /translation="EDEALMALDSRESEPDHRRACRPFSDNCGFTVAEASIFTVLMDD
                     ELALQLGARILGSVGGVYVNADGYKKSIPGPGIGNYLTVAKAMSLSRKLLGDESLQHR
                     TYMLAHGTGTPQNRVTESHILNELAKVFGIERWPVAAIKAYVGHSMAPAGGDQLAAAL
                     GLWQHGWLPGITTIDHIADDVHDSNLNLPLEHLELGAENVDAAFVNSKGFGGNNATGL
                     FLSPAKTREMLRKRWGKKALAEHGRSNESVEQAALDYDEAMVRGEAKPIYQFGEGVVH
                     GEDLTISRDSIHIPGFGMPVDLDLENPFPDMT"
BASE COUNT          505 a          929 c         1018 g          550 t
ORIGIN      
        1 ccgccatcat ggtcatcgac ggctccaagg gcgtcgagcc gcgcacgatc aagctcatgg
       61 aagtctgccg gctgcgcgat acgccgattc tcacgttcat caacaaactc gaccgggaga
      121 tccgggaccc ggtggaggtg atggacgaga tagaggacat cctgcagatt cagtgtgcgc
      181 ccatgacctg gccgatcggc atgggccgcc agttcaaggg catctacgat ctgtacgccg
      241 atgagatcgt ctgcttcgtt caggggcacg gccatcacat tttcgactac ccgcggatca
      301 agggcctgga gagcaacgcc gcgcgcgagc tgctgggttc ggaccacgat gatgtggtcg
      361 aagagatcga gctggtacgc ggggccagcc acgcgttcga cgtcgacgac tatctcggcg
      421 cgacgctgtc gcccgtctac ttcgggaccg cgctcggcaa cttcggagtc aaagagatgc
      481 tcgacgggtt cgtcacgtac gccccgcccc cccaggcccg acacgccgac gcccgcgacg
      541 tgaagccggg ggaggaacag ttcagcgggt tcgtgttcaa gatccaggcg aacatggacc
      601 cgaagcaccg cgatcgcatc gccttcctcc gggtgtgctc gggccgttat gcccagggca
      661 tgcggatgcg ccacgtgcgg ctcggaaagg acgtcaaggt tgccgatgcg gtgacctttc
      721 tcgccggtga ccgggcccag acggtggagg cgtttcctgg cgacatcatc gggcttcaca
      781 atcacggcac cattcagatc ggcgattcct tcacccaggg tgaggtgatc aagttcaccg
      841 ggatcccgaa ctttgcgccc gagctgttcc gacgggtgcg ggtaaaggat ccgctcagga
      901 gcaaacagct ggagcggggg ctggacgagc tgtcggagga gggagccagt caggttttca
      961 agcccttgat ccgcaacgag ctcatcgttg gtgcggtggg catgctgcag ttcgacgtgg
     1021 tggcgttccg tctgcgcgaa gagtacgggg ctgactgtgt ctacgattcg accaacatct
     1081 ttgctgcgcg ctgggtggtc tcggacgaca agaaaaagct cgaagacttc acgcgcaagc
     1141 accgcgatca gctggccgtg gatggcggag gacaccttgc ttaccttgct ccgacccggg
     1201 cgaacctgca gctggtggag gagcgctggc ccgagatccg ctttctcaag acccgggagc
     1261 attgattggc cggcctcgca gagcaggaac tgattcaagc ggtcagagcg tttgcctcgg
     1321 accctgaagc cgacgagctc gaagcggccg tgctcgtcaa tcgcgctctg gagccgtctc
     1381 tggatgcgga caccctgcgt gggcgcattg ccgagcttgc agcggcctgt ccggaaacgc
     1441 agacaccctg ggattacctc cgggagcttg gattcgtcgg caatgccgcg aattacgacg
     1501 cgctcgacaa cagccggctg gaccgtctgc tcgacaccgg gcggggcata cccatcgccc
     1561 tgggcgtcct gctgatcgag gtggcgcgcg ccagcggccg catggccgtc ggcgtcaact
     1621 ttcccgggca ctttctggtt cgggtgggag aaagtctggt agatccgttc cggatgacgc
     1681 agacctccga ggacgaatgc gttgccggtc tcggccaggc gcgagggaac ccgggcgcgg
     1741 ggggcgatct ctttccttcg gccaccgcgg cggccgtcct gctgcgcatg ctcaacaaca
     1801 tcaagtaccg ctacgcccaa gcgggggagt ggcatcgcag cctcgacatg gtggatctgc
     1861 agctagcggc cgcgggggag agcgcgcatc tgcttctcga acgcggtgag ctgtggttgc
     1921 ggctaggcgc caggggaccg gcacgtgccg atctggagcg cgcgttggct cttgctgggg
     1981 acggcgatcc ccgcttggcc gccgcggctc gacggttgct cgacgggctg gggtccggcc
     2041 cggagctgct gcactgattg cggggctcag cgcctcaggt catgtccggg aaaggatttt
     2101 ccagatcgag gtccacgggc attccgaagc cgggaatgtg gatgctgtcg cgactgatcg
     2161 tcaggtcctc gccgtgaacg acgccttcgc cgaactgata gatcggcttg gcttcgccgc
     2221 gaaccatggc ctcgtcgtag tccagggcgg cctgctcgac gctttcgttg ctgcggccgt
     2281 gctcggccaa cgccttcttg ccccagcgct tgcgaagcat ctcccgggtc ttcgccggtg
     2341 acaggaacag cccggtggca ttgttgccac cgaaaccttt ggagttgacg aaggcggcat
     2401 ccacgttctc ggcgccgagc tccaggtgct ccagcggcag gttcagattg ctgtcgtgaa
     2461 cgtcgtcggc gatgtgatca atggtggtga tgcctggcag ccagccgtgc tgccacaggc
     2521 ccagggcggc ggccagctga tcgcctcccg cgggcgccat ggagtggccc acgtaggcct
     2581 tgatggcagc tacgggccag cgctcgatac cgaagacctt ggccagctcg ttgagaatgt
     2641 gcgactcggt cacccggttc tgtggcgtcc cggtcccgtg ggccagcatg taggtgcggt
     2701 gctgcaggga ctcgtcgccg agcagcttgc gggagaggct catggccttg gccaccgtga
     2761 gatagttgcc gatgccgggg cccgggattg atttcttgta cccgtcggcg ttgacgtaga
     2821 cgccccccac cgagccgagg atgcgggcgc cgagctgcaa tgccagctcg tcgtccatca
     2881 gaacggtgaa gatgctcgcc tccgccacgg tgaatccgca gttgtcggag aacggtcgac
     2941 aggcgcgccg gtgatcgggc tcggactccc ggctgtcgag cgccatcaac gcttcgtcct
     3001 cg
//
LOCUS       DBNHKV010000270        13178 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1030469, whole genome shotgun sequence.
ACCESSION   DBNHKV010000270 DBNHKV010000000
VERSION     DBNHKV010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13178
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1030469"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="AC16PP_08490"
     CDS             <1..971
                     /locus_tag="AC16PP_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6481704.1"
                     /translation="GTRIDYLDPDSRPSINEMAALGQSVVGELDSDRTTIKIGFDYRF
                     TDDIMGYAHFSEGFKAGEFGARAGSLNTVGPTDDETSESFEIGLKSELLDGRLRANAT
                     FFHTTYKGLAFEVFFPSPDNPTGQETASQNIGEATTKGFELEVTAVPIDGLTLQGALG
                     ILDAEYDEFCADLNGPQPPGVEVNPTSDCGNVVPLPDGSYLVDVDYTDLKLSRAPKTQ
                     VYLSGMYEWNTDIGGMFVRAATSYESSYFADGALNHPTAKTGDFWLMDASAGWTSPND
                     QWRIQAWCKNCTDKHYTAGLTPTAQFFNQHFYGLPRTYGVTLSYRK"
     gene            968..2332
                     /locus_tag="AC16PP_08495"
     CDS             968..2332
                     /locus_tag="AC16PP_08495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spinster family MFS transporter"
                     /protein_id="HIR6481705.1"
                     /translation="MNEGTPASAGPSAADAADADAGRTGIGYAWYVVGVLLLAQTFSF
                     LDRMIMGLLVGPIRETFQISDTQYSLLAGLAFSLFYAVMGLPLARIADSRSRRNLIAV
                     GIAVWSAMTAVCGLARGFWTLFIARVGVGVGEATLAPGAYSMITDYFPRGLLARALSV
                     YMIGVTLGSGFAYMLGGAVVAYVEHMPQIVLPLLGEVYGWQLTFFIVGLPGILVSVLM
                     VLTVREPKRHGLTDGSQASIPLPEVVSFLWQRRRAYGGHIFGISIFIMVVYALNLWGP
                     SYLIRTFGYSRPEAGWIFGVVMIVAGTAGLLLAGTLADAWMKRGTRDAYVRTILLSIV
                     CITPCAVALGFIEDPALGILFIGLAVFFSAFQGGIAGGTLQLMTPNRMRGQVTAVYQL
                     VANLIGLGLGPTVVAATTDYVFGYDEAIGKSIALSAAVLCPVGGLLLWRSLSAIRGQL
                     EEQM"
     gene            complement(2385..3425)
                     /locus_tag="AC16PP_08500"
     CDS             complement(2385..3425)
                     /locus_tag="AC16PP_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481706.1"
                     /translation="MIELPLLPTSLVGSYAQPAWLIDRDRLGQRLPPRVLLDALWKVD
                     ALSLEEAQNDATVIALEDQHRAGVDIVTDGEMRRESYSNRFANALEGVDLDNPGAAVD
                     RTGKTVPVPRVVGEIRRREPVEAPFIAFLKRHTDRPIKITLPGPFTMAQQAQNDYYAD
                     EGSLAMAYATAVNAELKDLFAAGVDIVQLDEPYVQARPEAAREYAVASIDRALEGASG
                     PTVLHVCFGYGQHVADKPAGYAFLDELNDCRADEISIECAQPRLKMDLVQGLPNKKVH
                     VGVLDLRDQTPETPETVAERIRSALEFLPAERMVVAPDCGMKYLPREVAFAKLANMVR
                     GRDLVRADLAGP"
     gene            complement(3493..5256)
                     /locus_tag="AC16PP_08505"
     CDS             complement(3493..5256)
                     /locus_tag="AC16PP_08505"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6481707.1"
                     /translation="MSSVARCLSPYLGPALAAWGLLLCATNAGAATCTNATPGEAQLY
                     WGDLHVHTAYSLDAYAFGAIAAPKEAYAFGRGQPLRLANGEMVAIDRPLDFMAVTDHA
                     ETYDVMYICTDPLYRDDAYCRAIRSGPEPGNARTVFNDYLLPLVSDQPPQMAAVCDED
                     GVDCRAASIGQWRRAQRAANAANDPCTFTALIGYEWTASPGGHHWHRNVIFRSDRVPD
                     RAADYVHFPEVTALWRELDTHCRSEDGCEVLTIPHNINWADGGRTFDIEHASEADLAA
                     RARFERLAEIHQEKGNSECLPAHRDDPGSDCAFELLTDNAAKARLTGPEDISPEDAWR
                     RMRSTYYRGLLARGLAAYASSESGLNPFMLGAIGSTDTHFGTPGLVSEAGYPGGIASL
                     WLDGEERLTLPNYNPGGLVAVWAQENTRAAIFDSLQARSAYATSGTRIKLRFGATEHD
                     ACENSKVNYDTFMGETLTTGLPSDNTAGPVFTVEAGRDEAPLGTVQIIKGELREDGVV
                     ETVHTVADFPDGRDAVCISWQDEAFTAAAPAYWYARVLELPAPRWTKLLCEATGSCDR
                     FPDADRNIRERAWSSPIWHLP"
     gene            complement(5253..6368)
                     /locus_tag="AC16PP_08510"
     CDS             complement(5253..6368)
                     /locus_tag="AC16PP_08510"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6481708.1"
                     /translation="MASWQRQFVSTTSPTGTRNGAGFYPCQGLYYAPTGSRPRTALIA
                     THYNVDFSEHYLGEFMAARGYGFLGWNTRFRGAEAFFLLEHALVDIGTGVRWLKEEAG
                     VERVVILGNSGGGSLMGAYQSQALGVTMTATPGLSLPDAIHELIPADLYISLCAHLGR
                     PEVLTDWFDPSVTDETDPTSIDPFLNMYEPANGPPYAPGFVKRYRAAQRERNDRITRW
                     CHAELARLAELGMFDRAFNLYRTWADLRLMDGSLDPSEREVGRCYAGDPKTANFSPRG
                     IGLTNTLRTWLSMWSLRDSHCRGAPHLNRITQPALVIQSLADTGVFPGDARAIFDALG
                     SKDKHLEMITGDHYLENPRDARDGVADMIADWLASHT"
     gene            6608..7117
                     /locus_tag="AC16PP_08515"
     CDS             6608..7117
                     /locus_tag="AC16PP_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6481709.1"
                     /translation="MIGQSRTLQSSNEPVGVMADLVRALDWFDNGLQNVLASLGYKPL
                     HRTQSMIMVHVASGVESPADIARSMGLSRQNVHHMAKALIADGLIEQAPDPADPRRTL
                     YRLAGSAAEIRRAALETLAQLELVLAGRIGLRSLHALRGALGKDWGDAIAGGEALAAE
                     LDGHDVLAN"
     gene            7129..8292
                     /locus_tag="AC16PP_08520"
     CDS             7129..8292
                     /locus_tag="AC16PP_08520"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6481710.1"
                     /translation="MHEREFVERIRGLVPMLEAHAGEAERDRKPVDSVMAAVEETGAY
                     RFFVPKRYGGWEFGLDTFMDVGLLLGEGCVSTAWVVTFCMEHNWLLGLFNQQAQDEIF
                     GEFPYIIAPGTLAPRGTAEPVDGGYRVSGRWQWGTGVMHANWVMVGALTPSADPEKPD
                     LCMYLIPREEVDVLDTWHVAGMVGTGSNDIEVKDVFVPGHRMQSVSHMRDGGSPGAAL
                     HGTPTYRMPMMPVLGLTAAAPAVGGARRAVALFRELLGERNVYGTTEKQGQRAIAQAR
                     LAHAQIAVENAQILLKDTARRVMEWGESGERCPDVERAHLRLKIATVVRQARDVVQQV
                     VEACGAGAHFLDSPLQRIQRDLNTLSCHTVFDMDVGGELYGRLLLGLPANAPV"
     gene            complement(8307..8759)
                     /locus_tag="AC16PP_08525"
     CDS             complement(8307..8759)
                     /locus_tag="AC16PP_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6481711.1"
                     /translation="MAPSTSDPDRSYGYQSRATHRAFDRLLQQHLRQHGLTNGYWYVL
                     RVLWEEEGMSQRELARASNLTESSAVIMLDGMQRAGLVARRKDPADGRRQRVFLTAKA
                     KRLKPKLLPVAGELNALAASGIAAEDLATYLAVAHRMRANLVAALETG"
     gene            complement(8761..9381)
                     /locus_tag="AC16PP_08530"
     CDS             complement(8761..9381)
                     /locus_tag="AC16PP_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR6481712.1"
                     /translation="MFYDPREDPQPEPLGHNPINALVAPRPIGWIASLSADGAPNLAP
                     FSYFNAFSADPPVVAFAPNEKSGAGGPKDTLANVRSVPEFTASLVSEPLARRMNESSR
                     VLPHGVSEFIEAGLTPAASRLVAPPYVGEALAALECRVFDIIELPGRPNGRRSHLVIG
                     EVVGIHIDDGVIVDGRIDSLALAQLARLGYHDYTVVRELIEIPRPD"
     gene            complement(9402..10511)
                     /locus_tag="AC16PP_08535"
     CDS             complement(9402..10511)
                     /locus_tag="AC16PP_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6481713.1"
                     /translation="MSDRAPLIVDRTGEPLTTSEPARSKPARDWPPITITREMIDEEI
                     ERLADLPQPANGRRQAMFVHPNAPDPGRGLTPGVQVTLDVLKPGEETVPVRHNSTQVS
                     FCIRGGGTAIVGDRPIRYAQYDAWNHPSYATYRHVNDSRELQARLTYSNAALLEMMRV
                     HLVEEDPQDEPVARSAQAPAATGAQTETFRIGEDGAALMPYETLINPPSVASDALHWP
                     WRTVKANLDRLEALGADYVGRRLYLLFNPRTERFNGTTPNFFATITIRPPGIVDKPHR
                     HVSSAINYYFNGSGYSRVEGKRYDWKAGDLMVSAPGWAVHNHASNEGDPVYELTIQDQ
                     PLNIYMESLLWQEDLAEPARILGSHEGFSTNRSTL"
     gene            10611..11843
                     /locus_tag="AC16PP_08540"
     CDS             10611..11843
                     /locus_tag="AC16PP_08540"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6481714.1"
                     /translation="MDVDGATWSWPDGGVTEVPYAIYTDPGIFAREMERIFSGPSWAY
                     VGLEAEIPQPGDYLVTRIGVRSVVIARDEEGAIHGFANRCAHRGVKVCRQRHGNARFF
                     VCPYHQWSYDARGRLRGVPFINGVRQQGGMPEGFAREDHNLPPLDIATRGGVIFASFS
                     ATAPRLEDYLGDTNLSYFDRVFDGRQLKVLGYSRQRIPGNWKLMFENIKDPYHASLLH
                     VFLVTFGLFRADQPAQVRMDPSGAHGLLISRRGEQGATADTGEMSSMKEDFSLADARL
                     LDPVKEFPDDATVVMQTIWPNLIVQQQSNTLALRQIQPLDPGRFELHWTFFGYGDDDD
                     AMTLRRLRQANLMSSAGLVSADDSEVIKLAQDGVAQYPDDVGVMLMGGRGTDDEDHMV
                     TETAIRAFYAHYRRVMGL"
     gene            11840..12319
                     /locus_tag="AC16PP_08545"
     CDS             11840..12319
                     /locus_tag="AC16PP_08545"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013062.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HIR6481715.1"
                     /translation="MTLAAEERAELEEVYARYADLLDDGPLADWPALFHEECLYLLIP
                     RENHDRGLPLAIMRCESRGMLEDRVRAVQETIMHEPRYLRHHITNVRGARTDDGGYSV
                     IANYSVIEVLQDELPRILSVGRYLDVLTRSPTGALMFREKRVVFDSVMVPNTIVYPL"
     gene            12329..>13178
                     /locus_tag="AC16PP_08550"
     CDS             12329..>13178
                     /locus_tag="AC16PP_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR6481716.1"
                     /translation="MTLSDRLLDLGFLEAETPRTPMHFSALMFFDTPSDPAPFVSGLR
                     EAWTGAVPVAPFDLVPVHDLKSGLFLPRLAPAERVDVEDHLHLVTLPAGSGEQALLEL
                     AQRLHAECLDRSRPLWDVYLITGFGERCFAMFVKIHHAIFDGHGGLRLFVETLEEAPV
                     TRLPEPFWSRPSAAAPGHGRPLGPFEAVGALARSAGGLFRNAAVGARHASRWLLGSAP
                     VAPPAFSEPASILNGPSSGRRRLAHLSLPLPEIERCAADHGASVNDVLLAICGGALRR
                     YLEARSA"
BASE COUNT         2098 a         4201 c         4476 g         2403 t
ORIGIN      
        1 tcggcacccg cattgactac ctggatcccg attctcgacc cagcatcaac gagatggctg
       61 ccctcggcca gtcggtggtc ggcgagctcg acagcgaccg aaccaccatc aagatcggct
      121 tcgactatcg cttcaccgac gacatcatgg gttacgcgca cttctcggaa ggcttcaagg
      181 ccggcgagtt cggcgcccgg gccgggtcgc tgaacacggt tgggcccacc gacgacgaga
      241 cctcggagtc gttcgagatt ggcctgaaga gcgagctgct cgacggccgg ctgcgcgcca
      301 acgcgacgtt cttccacacc acctacaaag gcctggcatt cgaggtgttc ttcccgtcgc
      361 cggacaatcc caccgggcag gagacggcgt cgcagaacat cggcgaagcc accaccaaag
      421 gattcgaact ggaagttacc gcggtcccaa tcgacggcct gacgctgcag ggagcgttag
      481 gtattctcga cgccgagtac gacgagttct gcgccgacct gaacggaccg caaccgcctg
      541 gggtcgaagt gaaccccacc tcggattgcg ggaatgtggt gccgctgccg gatggctcct
      601 acctggtgga tgtggattac acggatctga agctcagccg cgcgccgaag acgcaggtct
      661 acctctccgg catgtacgaa tggaataccg atatcggcgg catgttcgtg cgcgcggcaa
      721 cgagctacga aagcagctac ttcgccgacg gcgcgctcaa tcaccccacg gcgaaaacgg
      781 gtgatttctg gctgatggac gcaagcgccg gctggaccag ccccaacgat cagtggcgca
      841 tccaggcctg gtgcaagaac tgcacggaca agcattatac ggcaggcctc acgcccaccg
      901 cccagttctt caaccagcac ttctatgggc tgccacgcac ctacggcgtg acgctgtcat
      961 accgtaagtg aacgagggca cgcccgcgtc agccgggcct agcgcagctg acgcggcgga
     1021 tgcggatgcc gggcggaccg gcatcggtta cgcctggtac gtcgtcggcg ttctgctgct
     1081 tgcgcagacc ttctcctttc tcgaccggat gatcatgggt ctcctcgtgg ggcccatccg
     1141 ggagaccttc cagatcagtg acactcagta cagcctgctg gcgggtctgg ccttttctct
     1201 tttctacgcc gtcatgggcc tgccgctcgc tcgcatcgcg gacagcagga gccggcgcaa
     1261 cctgatcgcc gtgggcatcg cggtctggag cgcgatgacg gccgtgtgcg gcctggcccg
     1321 aggcttctgg acgctgttca tagcccgggt aggcgtcggc gtgggcgaag cgacgctggc
     1381 gccgggcgcg tactccatga tcaccgacta ctttcccaga ggccttcttg cccgggcgct
     1441 atccgtgtac atgatcgggg tgacgttggg ctccggtttc gcctacatgc tcggcggcgc
     1501 ggtcgtggcc tacgttgagc acatgcccca gatcgtcctg cccctgctcg gagaggtgta
     1561 cggctggcag ctcaccttct tcatcgtcgg tctgccgggc attctggtgt ccgttctcat
     1621 ggtgctgacc gtgcgtgagc cgaagcgcca cggcctgacc gacggctcgc aggcctccat
     1681 accccttccc gaggtggtca gctttctctg gcagaggcgc agggcctatg gcggtcacat
     1741 cttcggcata tccatcttca tcatggtcgt ctacgcgctc aatctctggg ggcccagcta
     1801 cctgattcga accttcggtt acagccgccc ggaagccggc tggatcttcg gtgtcgtgat
     1861 gatcgtggcg ggaaccgccg ggctcctgct cgcaggcacc ctggcggatg cctggatgaa
     1921 gcgtggcacc cgggatgcct acgtgcggac gatccttctc agcatcgtct gcatcacccc
     1981 gtgcgcggtt gcgctcggat tcattgaaga tcctgcgctg ggaattctct tcatcgggct
     2041 ggccgttttc ttttcagcct ttcagggcgg aatcgcgggc ggcacgctgc agctcatgac
     2101 gccgaaccgg atgcgcggcc aggtcaccgc cgtttatcag cttgttgcca acctaatcgg
     2161 gctgggcctg ggaccaacgg tggtggctgc caccaccgac tacgtgttcg gctacgacga
     2221 ggccatcggc aaatccatcg cgctgtcggc cgccgtcctc tgtccggtcg gcgggctgct
     2281 gctctggcgc agcctgtcag ccattcgcgg gcagctggag gagcagatgt gatcccgccc
     2341 ggatgacggc ggcgtagagc ttcccggact cggaaaggag ccgttcaggg tccggccagg
     2401 tcggccctaa cgaggtcgcg gcctctcacc atgttggcca gcttcgcgaa ggcgacctct
     2461 ctgggcaggt acttcatgcc gcagtcggga gcgaccacca tgcgctcggc gggcaggaac
     2521 tccagtgccg accggatgcg ttcggccacg gtttccggcg tctcgggagt ctggtcgcgc
     2581 agatccagca cgccgacgtg caccttcttg tttggcaggc cctggaccag atccatcttg
     2641 agtcgcggct gcgcgcactc gatggagatc tcgtccgccc ggcagtcatt gagctcgtcg
     2701 aggaacgcgt agccggccgg cttgtccgcg acgtgctgtc cgtagccaaa gcagacgtgc
     2761 agcacggtcg gtccgctggc gccttccagg gcccgatcga tggacgccac cgcgtactcc
     2821 cgggcggctt ccgggcgcgc ctgcacgtag ggctcgtcca gctggacgat gtccacgcct
     2881 gcggcaaaga gatccttgag ctccgcgttg acggccgttg cgtacgccat ggccagcgat
     2941 ccttcatctg cgtagtagtc gttctgcgcc tgctgcgcca tggtgaacgg gcccggcagg
     3001 gtgatcttga tgggccgatc agtgtgccgc ttgagaaaag cgatgaacgg ggcctccacg
     3061 ggctcgcggc gcctgatctc gccgaccacg cgcggcaccg gcacggtctt tcccgtccgg
     3121 tccacggctg cgccgggatt gtcgaggtcc acgccctcca gggcgttggc gaaccggttc
     3181 gagtagcttt cccgccgcat ctcgccgtcg gtgacgatgt ccacgccggc gcgatgctga
     3241 tcctccaggg cgatgaccgt ggcgtcgttc tgggcctctt cgagggaaag ggcgtccacc
     3301 ttccacaggg cgtcgagcag gactctgggc ggcagccgct ggccaagccg atcgcgatct
     3361 atgagccacg cgggctgtgc gtagctgccg accagcgagg tcggtagaag gggtagttcg
     3421 atcatcaggg ctcctcggat ctccgttgcg tgttgtctcg cgtattcgga atgacggcgc
     3481 tttcctcggg tttcatggca ggtgccagat gggtgaggac catgcccgct cccggatgtt
     3541 ccgatccgca tcggggaagc ggtcgcacga cccggtagct tcgcagagca gcttggtcca
     3601 gcgtggcgcg ggcaactcca ggacgcgcgc gtaccagtag gcgggcgcgg cggcggtaaa
     3661 tgcctcgtcc tgccacgaaa tgcagaccgc gtcgcggccg tccggaaagt ccgccacggt
     3721 gtggacggtt tccaccacac catcctcgcg aagctcgccc ttgatgatct gcacggttcc
     3781 cagcggcgcc tcgtcgcggc cggcctcaac ggtgaacacg ggcccggcgg tattgtcgga
     3841 cgggagcccg gtcgtcaggg tctcgcccat gaacgtatcg tagttcacct tggaattctc
     3901 gcaggcgtcg tgctcggtcg cgccgaagcg cagcttgatc ctagtgccgc tggtggcgta
     3961 ggcgctgcgc gcctgcaggg aatcgaaaat ggcggcgcgg gtattctcct gtgcccacac
     4021 ggccaccagc ccgcccggat tgtagttcgg cagcgtcagg cgttcctcgc cgtccagcca
     4081 cagggaggcg atgccgccag gatagccggc ctcactcacc aggcccgggg tgccgaagtg
     4141 ggtgtcggta gagcctatgg cgcccagcat gaaggggttg agtccggact cgctgctcgc
     4201 gtaggccgcc aggccccggg cgagcaggcc gcgatagtag gtcgagcgca tgcgtcgcca
     4261 ggcgtcttcg ggggagatgt cctcggggcc ggtcagtcgc gccttggcgg cgttgtcggt
     4321 caggagctcg aacgcgcagt cgctgcctgg atcatcccgg tgggcgggca gacactcgct
     4381 gttgcccttc tcctggtgaa tttccgccag ccgttcgaaa cgcgcccggg ccgccagatc
     4441 ggcttcgctc gcgtgctcga tgtcgaaagt gcggccaccg tcggcccaat tgatgttgtg
     4501 gggaatggtg agcacctcgc agccgtcctc gcttcgacag tgggtgtcca attcccgcca
     4561 cagggcggtg acctcgggaa aatgcacgta gtcggcggcg cgatcgggga cccggtcact
     4621 gcggaagatc acgttgcggt gccaatggtg gccgccgggt gaggcggtcc attcgtagcc
     4681 gatcagggcg gtgaaagtgc agggatcgtt ggccgcgttg gccgcgcgct gcgcgcgtcg
     4741 ccactgaccg atgctggcag cgcggcaatc gacgccgtct tcgtcgcaga ctgcggccat
     4801 ttgcggaggc tggtcgctga ccagcggcag aaggtagtcg ttgaagacgg ttctggcgtt
     4861 gccgggttcg gggccgctgc gaatggcccg acagtaggcg tcatcccggt agaggggatc
     4921 ggtgcagatg tacatcacat cataggtctc tgcgtgatcg gtgactgcca tgaagtccag
     4981 tggacgatcg atggccacca tctccccgtt ggccagacgc agaggctgac cgcggccgaa
     5041 cgcgtaggcc tctttgggcg ctgcgatggc gccaaatgcg taggcgtcga gggagtaggc
     5101 cgtgtgaacg tgcagatcgc cccagtacag ctgagcctcc cccggggttg cgttggtgca
     5161 ggtggcggcg cccgcgttgg tcgcgcagag gagcaaaccc catgccgcca gcgcaggccc
     5221 caggtacggt gacagacagc gggcaactga gctcacgtgt gcgaggcgag ccagtcggcg
     5281 atcatgtcgg caacgccgtc gcgtgcatcg cgcggattct ccaggtagtg atcgccggtg
     5341 atcatctcca ggtgtttatc cttgctcccc agcgcatcga atatcgccct ggcatccccc
     5401 ggaaagacac cggtgtcggc cagactctgg atcacgaggg ccggctgggt gatgcggttc
     5461 aggtgcggcg ctccccggca atgggagtcc cttaagctcc acatggacag ccacgtccgt
     5521 agcgtgttgg tcaggccaat accccgaggg ctgaagttgg ccgttttggg gtcgccggcg
     5581 tagcagcggc cgacttccct ttccgatggg tccagagagc cgtccatcaa ccggagatcg
     5641 gcccaggtgc ggtagaggtt gaaagcgcgg tcgaacatgc cgagctcggc gagccttgcc
     5701 agctctgcgt ggcaccagcg ggtaattcgg tcgttgcgtt cccgctgcgc tgcccgatag
     5761 cgcttcacga atccgggcgc gtagggtggc ccgttggccg gttcgtacat gttcaggaac
     5821 gggtcgatgc tcgtcggatc ggtctcgtcc gtcacggaag ggtcgaacca gtcggtgagc
     5881 acttccggac ggccgaggtg ggcacacaga gagatgtaga gatcggccgg gatgagctcg
     5941 tggatcgcat cgggcaagga cagaccgggg gtcgccgtca tggttacgcc gagtgcctgg
     6001 gactggtagg cgcccatcag ggagccgccg ccggagttgc cgaggatgac cacccgctcc
     6061 acgccggctt cttctttcag ccagcgcacg ccggttccga tgtcgacgag agcgtgttcc
     6121 aggaggaaga aggcttccgc tccgcggaaa cgggtattcc agcccaggaa gccgtacccg
     6181 cgtgctgcca tgaactcgcc caggtagtgc tcgctgaagt ccacgttgta gtgcgtcgcg
     6241 atcagggccg tccggggccg cgagcccgtc ggggcgtagt acagaccctg acagggataa
     6301 aaaccggcgc cgttgcgggt gccggtcggt gaggtcgtgg aaacgaactg gcgctgccaa
     6361 ctggccattg gcgatccccc cggaagcctg aggcaccgac attacgggat atatccggag
     6421 ctttcaaagc ggcgttggct ctggcgacgc tgttcgggac ccgtggttct gtgtaagatc
     6481 atttgacgct aactgggcgg gctggatgct ttgcgcgcgg cccgattcgc cctcggcgaa
     6541 tcgtgagaaa gtgctcgatg gcgacgtcgg caccgggaag atcagatccg gaaattcggg
     6601 gttaacgatg attgggcagt cgcgaacgct gcaaagcagc aacgaaccgg ttggcgtgat
     6661 ggccgatctg gtgcgcgcgc tcgactggtt cgacaacggc cttcagaacg tgctcgcctc
     6721 gctgggctac aagccgctgc accggaccca gtccatgatc atggttcacg tcgccagcgg
     6781 ggtcgagtcg ccggcagaca tcgcccgatc gatgggcctg agccgccaga acgtgcatca
     6841 catggccaaa gcgctcatcg ccgacgggct catcgaacag gcgccggacc cggcggaccc
     6901 gcggcgcaca ctttatcggc tggcgggctc cgccgcggag atccgccgcg cagctttgga
     6961 gactctggct cagctcgagc tcgtgctcgc tggacgcatt gggctccgca gcctccatgc
     7021 gcttcggggc gccctcggca aagactgggg cgacgcgatt gccggcggtg aagcgctcgc
     7081 ggcagagctc gacgggcacg acgtgttggc taactgaggt aggcgggtat gcacgaacgg
     7141 gaatttgtcg agcgcatccg cgggctggtg ccgatgctgg aggcccatgc cggcgaggcc
     7201 gagcgcgatc gcaagcccgt ggattccgtg atggcggcgg tcgaggaaac cggcgcgtac
     7261 cggttcttcg tgccgaaacg ctacggcggc tgggagttcg ggttggacac gttcatggac
     7321 gtcggcctcc tgcttggcga aggctgcgta tccaccgcct gggtggtgac cttctgcatg
     7381 gagcacaact ggctgctggg cctgttcaac cagcaggccc aggacgagat cttcggtgag
     7441 tttccctaca tcatcgctcc cggcaccctc gcgccgcggg gaactgcaga gccggtggac
     7501 ggcggctatc gtgtgagcgg tcgatggcag tgggggaccg gggtgatgca cgccaactgg
     7561 gtcatggtgg gagcgctcac gccgtcggcg gatccggaaa agcccgacct gtgcatgtac
     7621 ctgattccca gggaggaagt ggacgtcctc gacacctggc acgtggcggg aatggtaggc
     7681 accggcagca acgacatcga ggtgaaggac gtcttcgtcc ccggccaccg gatgcagagc
     7741 gtgtcccaca tgcgcgacgg tggcagcccg ggggcggcgc tgcacggcac cccgacgtac
     7801 cgcatgccca tgatgcccgt cctgggcctg acggccgccg cgcccgccgt gggcggcgcg
     7861 cgcagggcgg tggcgctctt tcgcgagctg ctcggcgagc gcaacgtcta cggcacgacc
     7921 gaaaagcagg gccagcgtgc cattgctcaa gcccggctcg cccatgctca gatcgccgtc
     7981 gagaacgccc agatcctgct caaggacacc gcgcgccggg tgatggagtg gggagagagc
     8041 ggggagcgtt gtcccgacgt cgagcgtgcc catctgcgcc tgaagatcgc caccgtcgtc
     8101 cgccaggccc gcgacgtggt tcagcaggtg gtggaagcct gcggcgccgg cgctcacttt
     8161 ctggacagcc cgctgcagcg aattcagcgt gacctgaaca cgctgtcctg ccacacggtg
     8221 ttcgacatgg acgtgggcgg tgagctctat ggacgcctgc tgctcggact gcccgccaac
     8281 gctccggtct agggcccgtc gctcgatcag cccgtttcga gggcggcgac cagattcgcc
     8341 cgcatccggt gggccacggc caggtatgtc gccaggtcct cggcggcgat gccgctcgcc
     8401 gcaagggcgt tgagctcgcc cgctaccggc agcagcttcg gtttcagtcg tttcgccttt
     8461 gccgtcagga agacgcgctg gcgccgacca tccgcaggat cctttctgcg cgccaccagg
     8521 cccgcgcgct gcatgccgtc cagcatgatc accgcggaag actcggtcag gttggacgct
     8581 cgagccagct cccgctgtga catgccctcc tcctcccaca acacgcgcag cacgtaccag
     8641 tagccgttgg tgagaccgtg ctggcgcagg tgctgctgca gcagccggtc gaaggcgcgg
     8701 tgggtggccc gcgactggta accgtagctg cgatccgggt cgctggtaga cggcgccatc
     8761 tcagtcgggc ctcggaattt cgatcagctc ccgcaccacg gtgtagtcgt ggtaacccag
     8821 gcgggcgagc tgagcgaggg cgagagagtc gatgcgcccg tcgacgatca ccccgtcgtc
     8881 gatgtggatc ccaaccacct cgccgatgac caggtggctg cggcgtccgt tcgggcggcc
     8941 cggcagctcg atgatgtcga acacgcggca ttccagcgcg gccagcgctt cgcccacgta
     9001 cggcggggcc accaggcgcg acgccgccgg ggtcaatccg gcttcgatga actcgctgac
     9061 gccgtggggc agcacgcggg acgattcgtt catccgccgg gccagaggtt cggagacgag
     9121 gctcgcggtg aactcgggca cgctcctgac gttggccagc gtatccttgg gaccccccgc
     9181 gccggacttc tcgttggggg cgaaggccac gacgggcggg tctgccgaga acgcgttgaa
     9241 gtaggagaac ggtgccagat tcggcgcgcc gtcggcggag agggaggcga tccacccgat
     9301 gggccttggc gcgacgaggg cgttgatcgg attgtggccc agcggctcgg gctggggatc
     9361 ttctcgcgga tcgtaaaaca tgtttcgggc gtcagcagcg atcagagcgt cgaacggttg
     9421 gtggagaatc cctcgtgaga tccgagtatt cgggcaggct cggccaggtc ctcctgccac
     9481 agcagcgact ccatgtagat gttcagcggc tgatcctgaa tggtcagctc gtacaccggg
     9541 tcgccttcgt tggaggcgtg gttgtgcacg gcccagcccg gcgcggacac catgagatca
     9601 cctgccttcc agtcgtagcg tttgccctcg acgcggctgt aacccgaacc gttgaagtaa
     9661 tagttgatgg cggaggacac gtggcgatgg ggtttgtcca cgattcccgg cggtcgaatc
     9721 gtgatggtgg caaagaagtt cggcgtggtg ccgttgaacc tctcggttcg gggattgaac
     9781 agcaggtaga ggcggcgtcc cacgtagtcg gctcccagcg cttccagcct gtccagattt
     9841 gctttcaccg tccgccaggg ccagtgcagc gcgtcggagg ctaccgacgg cgggttgatg
     9901 agggtctcgt agggcatcag cgccgcgccg tcctcaccga tgcggaaggt ctccgtttgc
     9961 gccccggtgg ccgcgggcgc ctgggcgctc cttgccaccg gctcgtcctg cgggtcctcc
    10021 tccaccagat gcacgcgcat catctccagc agagcggcgt tagagtaggt cagccttgcc
    10081 tggagctcgc ggctgtcgtt gacgtgccgg taggtggcgt aggaggggtg attccaggcg
    10141 tcgtattgcg cgtaccggat gggccggtcg ccgacgatgg ccgtgccgcc gccccggatg
    10201 cagaagctca cctgggtgga attgtggcgg acgggaaccg tttcctcgcc gggtttcaga
    10261 acgtccaggg tcacctgaac gcccggggtg aggccccggc ctggatccgg cgcgttggga
    10321 tggacgaaca tcgcctggcg tcggccgttg gccggttgcg gcagatctgc cagccgctcg
    10381 atctcctcgt cgatcatctc ccgggtgatg gtgatcggcg gccagtccct ggccggcttt
    10441 gatcgggccg gctccgaggt ggtcagcggc tctccggtgc gatcgactat cagtggtgcg
    10501 cggtcgctca ttcttggcct ctcgagcggg aagctggacc ggcgaagccg gcgcgttgaa
    10561 atactttgat atcaaagtaa tatgtgggac gtcaactgcg gggttagctc atggatgtcg
    10621 atggcgcgac gtggtcgtgg cccgatggtg gggtgaccga ggtgccctac gccatctaca
    10681 cggaccctgg gatttttgcc cgggagatgg aacgcatatt ctcgggtccg agctgggcct
    10741 acgtcgggct ggaggcggag atcccccagc ccggggacta tcttgttaca cgaatcggcg
    10801 ttcggtccgt ggtgatcgcc agggacgagg aaggtgctat ccacggcttc gccaatcgct
    10861 gcgcgcaccg gggagtgaag gtttgccgcc agcgtcacgg caacgcgcga ttcttcgtct
    10921 gcccctacca tcagtggtcc tacgatgccc ggggccgcct gcgcggcgtt cccttcatca
    10981 acggcgtccg gcagcaggga ggaatgcccg aggggttcgc ccgtgaggat cacaacctgc
    11041 cgcccctcga catcgccacc cgtggcggcg tcatcttcgc gtccttctcc gcgacggcgc
    11101 cgcggctcga ggattacctc ggtgacacca acctctccta cttcgaccgt gtgttcgacg
    11161 gccggcagct gaaagtcctc ggctactcgc ggcagcgcat ccccgggaac tggaagctca
    11221 tgttcgagaa catcaaggat ccctaccatg cgtcgctgct gcatgtcttt ctcgtgacct
    11281 tcgggctgtt ccgggcggat cagcctgccc aggtgcgcat ggaccctagc ggcgcccatg
    11341 ggctgctgat ctcgcggcgg ggagagcagg gagcaacggc ggacaccggg gagatgtcca
    11401 gcatgaagga ggacttctcc ctggcagacg cgcgtctcct ggatccggtc aaggagtttc
    11461 cggacgacgc cacggtggtg atgcagacca tctggccgaa tctgatcgtg cagcagcaat
    11521 ccaataccct ggcgctgcgc cagatacagc ctctggaccc cggccgcttc gagctgcact
    11581 ggacgttctt cggctacggg gacgacgacg atgcgatgac gctgcggcgt ctgaggcagg
    11641 cgaacctgat gagctcggcc ggtctggtct ccgcggacga cagcgaggtg atcaagctgg
    11701 ctcaggacgg cgtcgcccag tatcccgacg acgtcggggt gatgctcatg ggcggccgag
    11761 gcactgacga cgaggaccac atggtcacgg aaaccgccat cagagcgttc tacgctcatt
    11821 accgacgggt gatggggctg tgaccctcgc cgccgaggag cgcgccgagc tggaggaggt
    11881 ctacgctcgc tatgccgacc tcctggatga cggcccgctg gcggactggc ccgcactctt
    11941 ccacgaggag tgcctctacc tgcttatacc cagagagaat cacgatcgag gcctgccgct
    12001 cgccatcatg cgctgcgaga gccgtgggat gctggaggat cgggtccgtg cggttcagga
    12061 aaccatcatg cacgagccgc gctaccttcg gcatcacatc accaacgtcc gcggtgctcg
    12121 caccgacgac ggtggttact cggtgattgc caactactcc gtcatcgagg tgctgcagga
    12181 tgagttgccg cgcattctga gcgtgggtcg gtatctcgac gttttgacaa gatccccaac
    12241 gggcgcgctg atgttccggg agaagcgggt ggtattcgat tcggtgatgg tgcccaacac
    12301 catcgtctac ccgctgtagg tcggcgcggt gaccctctcc gaccggctcc tcgatctcgg
    12361 cttcctggag gcggagacgc ctcgcacgcc catgcacttc agcgcgctga tgttcttcga
    12421 tacgccatcc gatccggcgc ccttcgtttc cgggttgaga gaggcttgga cgggcgcggt
    12481 tccggtggcg cccttcgacc tggttcccgt gcacgatctg aaaagcgggc tctttctgcc
    12541 gcggctggcc ccggccgagc gcgtggacgt ggaggatcat ctgcatctgg taacccttcc
    12601 ggccgggagt ggcgagcagg cgctcttgga acttgcccag cgcctccatg ccgagtgtct
    12661 cgaccggagc cgcccgctct gggacgtgta cctgatcacc ggcttcggcg agcgctgttt
    12721 cgcgatgttc gtgaagattc atcacgcgat cttcgacggg cacggcggcc tgcgcctgtt
    12781 cgtggagacc ctggaagaag cccccgtgac gcggctgccg gagccgttct ggtccaggcc
    12841 cagcgccgcg gcccccggcc atggccgccc tctgggtccg ttcgaggcgg tgggtgccct
    12901 ggcgcgttcg gccggcgggc tcttccggaa cgcggccgtt ggcgcgcgcc atgcctcccg
    12961 ctggctgctc ggttcggcgc cggtggcccc gcccgcattc agcgagccgg cgagcatcct
    13021 caacgggccc tccagcggtc gacgccgact ggcccacctg agcctgccgc tgcctgagat
    13081 cgagcgatgt gcggccgacc acggggcatc cgtcaacgac gttctgctcg ccatctgcgg
    13141 cggcgcgctg cgccgctatc tcgaagcgcg cagcgccc
//
LOCUS       DBNHKV010000271         6502 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_534340, whole genome shotgun sequence.
ACCESSION   DBNHKV010000271 DBNHKV010000000
VERSION     DBNHKV010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6502)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_534340"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..329
                     /locus_tag="AC16PP_08555"
     CDS             132..329
                     /locus_tag="AC16PP_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481717.1"
                     /translation="MKDPAVRSYPEQFATTPDLLHVVIPRIEIIAEMAVRDERDALND
                     VCLAVACVADAIGSAQLAAQG"
     gene            complement(355..1107)
                     /locus_tag="AC16PP_08560"
     CDS             complement(355..1107)
                     /locus_tag="AC16PP_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6481718.1"
                     /translation="MEKDDHIMTVTIERPDRLNALHPPANAELGEVFDDFAADDDMWV
                     AIITGRGRGFSAGNDLRYQAEGGERVPMPRGFGGLTSRFDLNKPVIAAVNGVAMGGGF
                     EIALACDLIIASEKALFALPEPKVGLAALAGGLNRLPRQIGPKRALGMILTGRHVPAE
                     EGLQLGFVNEVVPHDELLAATRRWAEQICACAPLSIRASKDVVYRSLDSASLQASMQA
                     QYDSVKAMVKSEDFIEGPKAFAEKRPPAWKGR"
     gene            complement(1153..1755)
                     /locus_tag="AC16PP_08565"
     CDS             complement(1153..1755)
                     /locus_tag="AC16PP_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6481719.1"
                     /translation="MASILFLRHGQIAANAKGRWNGSTDSELTWRGRRQARRTAKHLA
                     AREAEITAIYSSPLRRTRFTAEAAADRLGLPVTIADGLREYAIGEWEDLPFRHLAREH
                     DFVNTTIRDPDFAPPGGESLRDVADRVVPTILEIHQRHREDSKVLVVGHGAAMAVAFG
                     AILERNPARWTNYHFANCSLSELVISPAPYVNFFNSTQHL"
     gene            1849..2910
                     /locus_tag="AC16PP_08570"
     CDS             1849..2910
                     /locus_tag="AC16PP_08570"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF014477.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR6481720.1"
                     /translation="MCRLVYFEGVIVRSAPRLIRDGAKRWLVWTLVGLLAGCGDPGYQ
                     RHGGSAMGTGYRVTAKCPGEVGDQVTAVLDAVNADMSTYLPESTLSVFNRGPVGEWLP
                     VSADLIEVVEAAHDLSELSGGAFDVTVGPLVNLWGFGPNGAPAQRPAAARVEFVRAKV
                     GYHYLESRRAPPALRKSTDVYVDLSAIAKGFAVDRIAQRLFELECSDFLVDVGGEVRA
                     SGVNPQGAAWRIGVEVPDPDSFGAIQRVLRISGSGLATSGDYRNFLEVDGERFAHTID
                     PRTGYPVDHTLASVTVVHESAMLADGYATLLNVLGPQEGFSLAEGLGLPVLFLIRTPN
                     GFEERYTVGLEQFLGQTDY"
     gene            2912..3136
                     /gene="nqrM"
                     /locus_tag="AC16PP_08575"
     CDS             2912..3136
                     /gene="nqrM"
                     /locus_tag="AC16PP_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HIR6481721.1"
                     /translation="MTILLTFLCMMAVVGLMAIGVMFGRRPIAGSCGGLKNLGIGGEC
                     EICGGDPARCESPGDTRLAVDATNRARRSR"
     gene            3217..4440
                     /locus_tag="AC16PP_08580"
     CDS             3217..4440
                     /locus_tag="AC16PP_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6481722.1"
                     /translation="MIKAVALSVGSRYAFSRRSTLSFISLVAISGLALSVAVLVIVTS
                     VVNGFDRELRERVFGVLPHLSFVGREPLAFSLDDLEALRAIPGVRGIAPFVEGAALVA
                     VADGVGAVMVTGMEPESYESVSDVHQFVRGRMPQAGEYGVLLGDQVAQRLGVAIGDAV
                     TLVLPSATVTPAGLFPRQKRFVITGLLDSQSDLDGRAAYVRLTVAQKLFRLGDRIHGY
                     QARLDDLFAALEVGSAGVLALGPQRLFARSWMRTHGNLYQAIGMLKSTLFVLLSFLIA
                     VAAFNLISTLVMVVDQRSGDIAVLRTLGSDTSTLIWTFVILGVLLGGAGIAAGLGLGA
                     ALALALPGLYEWLTTLLGADLLAQYFVTYLPVELQPGDLLGIAVTAFLLCVLSTLYPA
                     RRAAGLQPFKVLAYE"
     gene            4433..5107
                     /locus_tag="AC16PP_08585"
     CDS             4433..5107
                     /locus_tag="AC16PP_08585"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6481723.1"
                     /translation="MSEPVLEARGLVKSFTQGTTRVVVLSGADLDVVAGERVAIVGRS
                     GSGKSTLLHLLAGLDDADAGSVIVGGKDMTAGSPAQRAHIRNAHMGFVYQFHHLLPEF
                     SALENVIMPLRLGGMDPAAAAERARGMLERVGLAERFEHRPSALSGGERQRVAVARAL
                     VGSPLVVLADEPTGNLDRDNAEHVFRMMCELSDEHGVGFVIVTHDEEMLGSMHRVLRL
                     ENGRLA"
     gene            5104..6312
                     /locus_tag="AC16PP_08590"
     CDS             5104..6312
                     /locus_tag="AC16PP_08590"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR6481724.1"
                     /translation="MSTSLWIALRYLVTRRRQFAAFVTWVSVAGLALGVLVLTVVVSV
                     MNGFDAELRHRILGSVPHLIIEGESVTDPNVGSLLDRPEIVGSFDFFLGSGMVTRSGA
                     VHPVTVYGLDAEGLTGLGEIGQAMRSGRLSGLFEIPRGIVIGEPLAAHLGLLPGDSVA
                     VVISEPTASGVQPRFGRYELVGTFEVGAVIDYSVVVVARDALDGLRLDEVGVNGARLV
                     LADPLDAPRLAAELAMARPAWNLVSWTDSYGELFQAVGLEKMVMFIILLMVVAVAAFS
                     IVSGQMMAVADKRADIAILSTMGASRRTILGIFLLQGVLVSGLGIAVGLALGTLTAEH
                     IGAVIAGVEDLLGFRFLAGTYFVQVPSEVEVADLVIIGVMSSCLCLASAWLPAHRAAI
                     MNPLEGLHPG"
     gene            complement(6278..>6502)
                     /locus_tag="AC16PP_08595"
     CDS             complement(6278..>6502)
                     /locus_tag="AC16PP_08595"
                     /inference="COORDINATES: protein motif:HMM:NF021363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HIR6481725.1"
                     /translation="WLKTHLGRIGYPLIVGSLVCGWVSGLTAMVVVRIAWRMHVIKRW
                     RERRRRRLERQMATDDSGGVTPDEAPRAGS"
BASE COUNT          964 a         1962 c         2241 g         1335 t
ORIGIN      
        1 tttcgaatcg tggcgctata tggaaataaa aatatataaa acaatggctt atcaaggtat
       61 ttcagactgg cacgaaacct gaatgccatg tgctgacggc cgcttagcgg gagatggacg
      121 agaggcagag catgaaagat ccggcagtca ggtcataccc cgagcagttt gcgaccaccc
      181 cggacctgct gcacgtggtc attccaagaa tcgagatcat cgccgagatg gcagtgcggg
      241 acgagcgcga tgcgctcaac gatgtctgcc tggctgtagc ttgcgtcgcg gatgccatcg
      301 gctccgccca gttggcggcg cagggatagc gccgccgctg gaattttcct ggctttagcg
      361 ccccttccac gccggcggcc gcttctccgc gaacgctttc ggaccctcga tgaaatcctc
      421 gcttttgacc atggccttga cgctgtcgta ctgggcctgc atggacgcct gcagcgaagc
      481 ggagtcgagg ctgcgataga ccacgtcctt gctggctcgg atcgacaagg gggcgcaggc
      541 gcagatctgc tccgcccacc ggcgcgtggc ggccagcagc tcatcgtgtg gaaccacctc
      601 gttgacgaag ccgagctgca atccttcctc ggcaggcacg tgacggccgg tgagaatcat
      661 gccgagcgcg cgcttcgggc cgatctgccg cggcaaccgg ttcaacccgc cggcaagcgc
      721 agcaagaccg accttgggtt cgggcagtgc aaagagggct ttttcagagg cgatgatcag
      781 gtcgcaggcc agcgcgatct cgaacccgcc gcccatggcc acgccgttga ccgcggcgat
      841 caccggtttg ttgagatcga aacggctggt caagcccccg aatccccggg gcatgggtac
      901 gcgctctcca ccttcggcct ggtagcgcag gtcgttcccc gcactgaacc ctctgccgcg
      961 cccggtgatg atcgccaccc acatgtcgtc gtcagcggcg aaatcatcga atacctcgcc
     1021 gagctcggcg ttggccggcg ggtgcagcgc gttgagacgg tcgggtcgct cgatggtgac
     1081 cgtcatgatg tgatcgtctt tttccacact gcagtactcg tacatgcgct ttcgttctcc
     1141 cgttgaggtc ggtcagaggt gctgggtcga gttgaagaag ttaacgtaag gcgcgggcga
     1201 aatcaccagc tcgctcaggc tgcagttggc aaagtggtag ttggtccagc gcgcggggtt
     1261 gcgttcgagt atcgctccaa aggcaacggc catggccgcg ccgtggccga cgacgagaac
     1321 tttgctatct tcccggtggc gttggtggat ttccagaatg gtgggcacga cgcggtcggc
     1381 tacgtcccgc aacgattcac cgccgggcgg cgcgaagtcg ggatcgcgga tggtcgtgtt
     1441 gacgaaatcg tgctcgcggg cgaggtgacg aaaagggaga tcctcccact cgccgatggc
     1501 gtattcccgc aacccgtcgg cgatggttac cggcagaccg agcctgtcgg cggctgcctc
     1561 ggccgtgaat cgcgtccggc gcagcgggct ggagtaaatg gcagtgatct ctgcttcccg
     1621 cgcggccagg tgctttgcgg tgcggcgtgc ctgccggcgc ccgcgccacg tcagctcgct
     1681 gtccgtggag ccgttccagc ggcccttcgc gttggcggct atctggccgt ggcgtagaaa
     1741 gaggatcgag gccatgttcg atttacgtca tgatgccggg cgccccacag ggacgcagcg
     1801 gtgtgaagcc cggtgtcggg cgcgatgcgg gcgggatggt atttcctggt gtgcaggctt
     1861 gtctacttcg agggtgtgat tgtgcgcagc gcgccgcgat tgatacgtga tggcgccaag
     1921 cggtggctgg tctggacgct ggtcggtctc ctggcgggat gtggcgaccc cggttatcag
     1981 cgccatgggg gcagtgccat ggggaccggc tatcgggtaa cggccaagtg cccgggtgag
     2041 gtgggcgatc aggtcaccgc cgtgctggat gccgtcaacg ccgacatgtc cacctacctc
     2101 ccggaatcca cgctttcggt gttcaaccgc ggtccggtcg gcgagtggtt gccggtgagc
     2161 gccgatctca tcgaggtggt ggaagcggcc catgacttga gcgagttgtc gggcggggcg
     2221 ttcgacgtaa cggtggggcc gctcgtcaat ctttggggct tcgggccgaa cggggcgccg
     2281 gcacagcgcc ccgcggcggc tcgagtggag ttcgtgcggg cgaaggtcgg ttaccattac
     2341 ctcgagtccc gccgggcgcc gccggctctg cgcaaatcca ccgacgttta cgtcgatctg
     2401 tctgccatcg ccaaggggtt cgccgtcgat cggattgcgc aaaggctctt cgagcttgaa
     2461 tgttccgatt ttctcgtcga cgttggcggc gaggttcgcg cctccggcgt gaaccctcag
     2521 ggcgcggcct ggcgaattgg cgtggaggtc ccggacccgg acagcttcgg cgcgattcag
     2581 cgggtgcttc ggatcagcgg gtctggcctt gccacttccg gcgactatcg caattttcta
     2641 gaggtcgacg gcgagcgctt tgctcacacc atcgatcccc gcacgggcta tccggtcgac
     2701 cacaccctcg cctcggtgac cgtggttcat gaatccgcca tgctggcgga cggttacgcc
     2761 acgcttctca acgtcctggg gccgcaagag ggcttttccc tggccgaggg gctgggatta
     2821 ccggtacttt tcctgatccg aactccgaac ggcttcgaag agaggtatac tgtcggcttg
     2881 gagcagtttc tcgggcaaac ggattattga cgtgacgatt cttctgacct ttctctgcat
     2941 gatggcggtg gtggggctca tggccatcgg cgtcatgttc gggcgccggc ccatcgcggg
     3001 cagctgtggt gggctgaaga acctggggat cggcggcgag tgcgagatct gcggcggcga
     3061 cccggcgcgc tgcgaatcgc ctggggacac ccgtcttgcc gtggacgcaa ccaaccgggc
     3121 acgacgctcc cgctgattac cagggcttct ccccgagggc gcgcggcgct accgacgtgt
     3181 tagcatgcgg cctcccccgg gccgggtcgg tttgcattga tcaaagcagt tgcgctcagc
     3241 gtgggcagtc gctacgcctt ctcgagacgg agcacgcttt ccttcatcag cctggtcgcc
     3301 atcagcgggc tcgccctcag cgttgccgtg ctggtgatcg tgacgtcggt cgtgaacggc
     3361 ttcgacagag aactccgcga gcgggttttc ggcgtgcttc ctcatctgag cttcgtcggg
     3421 cgcgagccgt tggcattcag cctcgatgat ctggaggcgc ttcgggccat tcccggcgtc
     3481 cgggggatcg ccccattcgt tgaaggcgcc gcgctggtcg cggtggccga cggggtgggc
     3541 gccgtgatgg tcacgggcat ggagccggaa agctacgaga gcgtctccga cgtgcatcag
     3601 tttgttcgcg gccgcatgcc tcaagccggc gagtacgggg tattgctcgg tgatcaggtg
     3661 gcccagcggc tcggcgtggc cattggcgat gctgtcaccc tggtgctgcc gtcggcgacg
     3721 gtcacgcctg cggggctgtt cccgcggcag aaacgctttg tgattacggg gctgctcgac
     3781 tcgcagtccg acctggacgg tcgcgcggcc tatgtgcgcc tgaccgtggc gcagaagctg
     3841 ttccggcttg gcgatcgcat acacgggtat caagccaggc tggacgatct gttcgctgcg
     3901 ttggaggtgg gttcggcagg tgttctcgcc ctcggtccgc agcgcctgtt cgcgcgttcc
     3961 tggatgcgta cccatggcaa cctctatcag gccatcggca tgttgaagag cacgctgttc
     4021 gtcctgcttt cgtttctgat cgcggtggcc gcgttcaacc tgatctcgac cctggtgatg
     4081 gtggtggacc agcgaagcgg cgacattgcc gtgctgcgga cgctgggcag tgacacctcg
     4141 acgctgatct ggacgttcgt cattctcggc gttctgctcg gcggtgccgg gatcgccgcg
     4201 ggtctcggcc tgggcgccgc gctggctctg gctcttcccg gcctgtatga gtggctgacg
     4261 acgcttctgg gagccgatct gctggcccag tacttcgtta cctacctgcc ggtcgagctg
     4321 cagccgggcg atcttctcgg catcgccgtg acggcctttt tgctgtgcgt tttgagcacc
     4381 ctgtacccgg cgcggcgcgc ggcggggtta cagcctttca aggttcttgc gtatgagtga
     4441 gccggtcctc gaagcccgcg gcctcgtcaa gtcgttcact cagggcacga cccgggtcgt
     4501 ggtgttgtcg ggggcggatc tcgacgttgt cgccggcgag cgggtcgcca tcgtcggccg
     4561 gtccggctcg ggcaagagca ccctgttgca tctgctggca ggtctcgacg atgccgacgc
     4621 ggggtcggtg atcgtcggcg gcaaggacat gacggcggga tctcccgcgc agcgggcgca
     4681 catccgcaac gcgcacatgg gcttcgtcta ccagtttcac catctgctgc cggagttctc
     4741 ggctctggaa aacgtcatca tgcctctgcg gctcggaggc atggaccctg ccgccgccgc
     4801 agagagagcg cgcggtatgc tggagcgagt gggtctggcc gagcggttcg agcatcggcc
     4861 atcagctctg tcgggcggag agcgccagcg ggtggccgtt gcccgcgcgc tggtgggcag
     4921 tccgctggtg gtgctcgccg acgagccgac ggggaacctc gaccgggaca acgcggagca
     4981 cgttttccgc atgatgtgcg agctcagcga tgagcatggt gtgggttttg tcatcgtcac
     5041 ccacgacgaa gagatgctgg gcagcatgca ccgagtcctg cgtctggaaa acggccggct
     5101 ggcgtgagca cgtcgctctg gattgctctg cgctatctgg tcacgcggcg ccgccagttt
     5161 gccgctttcg tcacgtgggt ctccgttgcc ggtctggcgc tgggcgttct ggtgctgacg
     5221 gtggtcgtca gcgtgatgaa cgggttcgat gccgagttga ggcaccgcat tctcggatcc
     5281 gtgccccatc tcatcatcga aggggagagc gtcaccgacc cgaatgtcgg ttcgctgctc
     5341 gaccggccgg aaatcgtcgg ctctttcgac ttcttcttgg gttcgggaat ggtcacccga
     5401 agcggcgcgg ttcatccggt gaccgtctac ggcctggacg cagaaggact caccgggctt
     5461 ggagagatcg gccaagccat gcgcagcgga cgtttgtccg gcctgttcga gatcccgcgg
     5521 ggaatcgtga ttggtgagcc gctggcggcg cacctgggcc tgctgccagg cgattcggtg
     5581 gcggtggtga tctccgagcc gacggcgtcc ggcgtgcagc cacgctttgg tcgctacgag
     5641 ctggtcggta ctttcgaggt cggcgccgtc atcgactact cggtggtcgt tgttgcccgc
     5701 gacgcgcttg acggcttgcg tttggacgag gtgggcgtca acggggcgcg cctggttctg
     5761 gcggatcccc tcgatgcgcc gcggctcgca gcggagctgg cgatggctcg gcctgcatgg
     5821 aatctggtca gctggacgga cagctacggc gagctctttc aggcggtggg cctggagaag
     5881 atggtgatgt tcatcattct gctcatggtg gttgcggtgg ccgcgttcag catcgtatcg
     5941 gggcagatga tggcggtggc cgacaagcgc gcggacattg cgattctgag cacgatgggc
     6001 gcttcgcgaa ggacgatcct cggcatcttc ctgctgcagg gagttttggt ctctggcctt
     6061 ggcatagccg tcggcctggc gctcgggact ttgacggccg agcatatcgg tgccgtgatc
     6121 gcaggcgtcg aggatcttct cggttttcgc tttctcgccg gcacctattt tgtccaggtg
     6181 ccctcagagg tcgaggtagc cgatctggtg atcatcggcg tcatgtcgtc gtgcctttgc
     6241 ctggcttcgg cctggttgcc cgcgcaccgg gccgcgatca tgaacccgct cgaggggctt
     6301 catccggggt gacgccgccg gagtcgtcag ttgccatctg gcgctccaga cgccgccgcc
     6361 gtctttcgcg ccaccgtttg atgacgtgca tccgccaggc gatgcgaacg actaccatgg
     6421 cggtgaggcc gctgacccac ccgcagacca gggatcctac aatcagcgga tagccgatgc
     6481 gtccgaggtg ggttttgagc ca
//
LOCUS       DBNHKV010000272         3293 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1383460, whole genome shotgun sequence.
ACCESSION   DBNHKV010000272 DBNHKV010000000
VERSION     DBNHKV010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1383460"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="AC16PP_08600"
     CDS             complement(<1..1006)
                     /locus_tag="AC16PP_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6481726.1"
                     /translation="MLKLLFQLSARNLLRHKRRNLMLLMAVVVAVGGVTLANALIRGF
                     QHDMREAAVQNLTGHLKVLAPGYLDDPGIQRSFTLVQGWSPDIGPRLLQGWAARIRIP
                     AVIMSERETRGVQFVGVDPAQETISFLGDASYRGEPLSGAADRRVVIGQALAEQLDTD
                     VGRRLVLITQGADGLNREAGFRIAGVYDADGQGLEKMFVFTGVTRLQELVDANVFTEV
                     SIKLREEPRGDGLKQGLAETFTDLDVMDWKELEPQTAAMFLFADTAIFIWFCIMMGAL
                     IFGLVNALITAVMERIREFGMLRAVGMRPGSVVVQVVLESTLIMAAGVALGLGLGFLL
                     V"
     gene            complement(1095..2363)
                     /locus_tag="AC16PP_08605"
     CDS             complement(1095..2363)
                     /locus_tag="AC16PP_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6481727.1"
                     /translation="MTQARTPAAGGRARPALLLRIGWRNLARNRRRTWLTAGGIAFAV
                     LLVVFSMAMQLGQYDIMMENATSLLTGHIQIQTADYVDSARFEDTMGSAGALRRVIEA
                     TAGVEAVAPRAEAFALASAGERSFGARIIGVDPDAERDTVRLLKMVREGRAIAAPDDG
                     LLGSLLARNLGVTLGDEVVLLGSEKHGGVAAMVITVAGILETGVADLDRVTLITPLAS
                     VQSAFGLADEVHTFAIRTEDLDRSEAVAESLRHSLTDRPALVVRHWSEVMPELRQAIE
                     IDRLGGELFFYVVEVLVVFSIVNTFIMTVFERTREFGMLKAIGMKPWRIVAMMQWEGF
                     FIWLLGASLGLGLAAIIVAWLQDVGIYMGETLEEYAEQFYMPARLYPAFSLQAFLSAP
                     LVMLMGTQIAALLPSLRIFRLNAAAALRGE"
     gene            complement(2360..3133)
                     /locus_tag="AC16PP_08610"
     CDS             complement(2360..3133)
                     /locus_tag="AC16PP_08610"
                     /inference="COORDINATES: protein motif:HMM:NF028441.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HIR6481728.1"
                     /translation="MHSVKRLLLTAAHSMLVIFPAGVLAAEEPTARELVAAAIDLTRG
                     QSSYAEFAMVIHRPDWQRTSKLVAWTRGRKDALIRFTAPARDAGNATLKQGEKMWTYN
                     PKLNRTIRLPFSLMSQSWAGSDFSYNDLSRTDKLLEEYELAITATRRSEGHTLYTIEA
                     VPREEAPVVWGKEEIVLRDDHVLISQTFFDQAMRPLKRMETLEIGEMGGRIFGTRMRM
                     SKLDEPENYTELSYARVDFDIELEDRIFTLFSLQSGQRP"
BASE COUNT          636 a         1112 c         1003 g          542 t
ORIGIN      
        1 cgaccagcag aaaacccaag ccgagcccca aggccacccc agcggccatg atgagggtgg
       61 attccagcac gacctgcacc accaccgagc cgggacgcat gccgacggct cgcagcatgc
      121 cgaactctct gatccgctcc atcacggcgg tgatcagggc gttgacgagc ccgaatatca
      181 aagcgcccat catgatgcag aaccagatga agatcgccgt atccgcgaac aggaacatgg
      241 ccgccgtctg cggttcgagt tccttccagt ccatcacgtc caggtcggta aaggtttccg
      301 ccagcccctg cttgaggcca tcgcctcgag gctcttcgcg cagcttgatc gacacctcgg
      361 tgaagacgtt ggcgtccacg agctcctgaa ggcgcgtcac gccggtgaag acgaacatct
      421 tctccagccc ctgaccatcg gcgtcgtaaa cgcccgcaat ccggaatccg gcttcgcgat
      481 tgagcccgtc cgcgccctgt gtgatcagca ccaggcgcct gcccacgtcg gtatcgagct
      541 gctcggccag cgcctgcccg atcacgaccc gccgatcggc tgcaccggat agcggctcgc
      601 cgcggtagct ggcatccccc agaaaggaga tcgtctcctg cgccgggtcc acgccgacga
      661 actgcacgcc gcgggtttcc cgttcgctca tgatgacggc cgggatcctg atgcgtgccg
      721 cccatccctg aagcagccgg gggccgatgt cgggcgacca gccctgcacc agagtgaagc
      781 ttctctgaat tcccgggtcg tccagatagc cgggcgcaag cactttcaga tgaccggtga
      841 ggttctgcac agcggcttct ctcatgtcgt gctgaaaacc gcgaatcagc gcgttggcca
      901 gggtcacgcc gccaaccgca accactaccg ccatgagcag catcaggttc cggcgcttgt
      961 gccgaagcag gtttcgagca gagagctgaa agagaagctt caacatggac gacaccggcg
     1021 cggcgacccg accaccctcc ctgtcctccg ggcagccatc gcaccgcccg cgttcgaagc
     1081 agcgtcaccc gacatcattc gcctctcagg gctgcggcgg cgttcagccg gaaaatgcgc
     1141 agagacggaa ggagcgcggc gatctgggtt cccatcagca tgaccagcgg cgcggagaga
     1201 aacgcctgaa gcgaaaacgc cgggtaaagc cgggccggca tgtagaactg ctcggcatac
     1261 tcctcgagcg tctcgcccat gtatatgccc acgtcctgaa gccatgccac gatgatggcg
     1321 gcgaggccca gccccagcga agccccgagc agccagatga agaacccctc ccactgcatc
     1381 atcgcgacga tgcgccaggg cttcatgccg atggctttga gcatcccgaa ttccctggtc
     1441 cgctcgaaga cggtcatgat gaaggtgttc acgatgctga acacgacgag cacctcgacg
     1501 acgtagaaga agagctcacc cccgagccgg tctatctcga tggcttgccg cagctccggc
     1561 attacctcgc tccaatgcct gaccacgagc gcgggccggt cggtcaaact gtgcctcaga
     1621 ctctcggcga cggcctcgct gcgatccaga tcctccgtgc ggatggcgaa agtgtgcacc
     1681 tcgtcggcaa gcccgaatgc gctctggact gacgcgagcg gggtgatgag ggtgacccga
     1741 tccagatcgg ctaccccggt ctccaggatg cccgccacgg tgatcaccat ggcggcgaca
     1801 ccgccgtgct tctcagatcc caagagcacc acctcatcgc cgagagtcac gccgaggttg
     1861 cgcgccagca aagagcccag caggccgtca tccggggcgg caatggctct accctcgcga
     1921 accatcttca gcaggcgcac ggtatcgcgc tcggcgtcgg gatctacgcc gatgatccgg
     1981 gcaccaaatg accgctctcc cgcggaagcc agcgcgaacg cttccgcgcg cggcgcgacg
     2041 gcctcaacgc cggcggtggc ttcgatcacc cgccggagcg caccggcgct ccccatggtg
     2101 tcctcgaagc gggcgctgtc gacgtagtcg gccgtttgaa tctggatgtg acccgtcagc
     2161 agggaggtgg cgttctccat catgatgtcg tactgaccga gctgcatcgc catggagaaa
     2221 accaccagca gcaccgcaaa cgcgatgcca cccgccgtca gccacgtccg cctgcggttg
     2281 cgcgccaggt tgcgccaacc gattcggagc agcagcgccg ggcgggcacg ccccccggcc
     2341 gccggcgtcc gcgcctgggt cacggccgct ggcccgactg cagcgagaag agcgtgaaga
     2401 ttcggtcttc cagctcgatg tcgaagtcga cccgggcgta gctgagctcg gtgtagtttt
     2461 ccggctcatc gagcttggac atgcgcattc gggtaccgaa gatccgcccg cccatctcgc
     2521 cgatctccag ggtttccatc cgcttgagcg gacgcatggc ctgatcgaag aacgtctgcg
     2581 agatcagaac gtggtcgtcg cgcagcacga tctcctcttt gccccacacc accggcgcct
     2641 cttcgcgggg aaccgcctcg atggtgtaaa gggtgtggcc ctcggatcgc cgggtcgccg
     2701 tgatggccag ctcgtattct tccaacagct tgtccgtgcg ggagagatcg ttgtatgaga
     2761 agtccgagcc tgcccagctc tgcgacatca ggctgaaggg caaccggatc gtgcggttca
     2821 gcttcgggtt gtaggtccac atcttctcgc cctgcttgag ggtcgcgttg cccgcatccc
     2881 tggccggcgc cgtgaaccgg atcagggcgt ccttgcggcc gcgggtccag gcgaccagct
     2941 tcgaggtccg ttgccaatcc ggccggtgaa tgaccatggc gaactccgcg taagacgatt
     3001 gcccccgggt gagatcgatg gcggcggcga ccagctcccg agcagtaggc tcttcggcgg
     3061 cgagaacgcc ggccgggaag atgacgagca tcgagtgcgc agcggtgagc agcagccgct
     3121 tgacgctatg cattgacaag cctccacgag caggcaatat ccggatcggc ctcaacgttt
     3181 gactctatca acaattattg tccccatgcc gctgcgagaa gagaagaaac gacaggtccg
     3241 gaaggacata ctagacgctg cgcgagagct gatcgagcaa cgcggatacg aca
//
LOCUS       DBNHKV010000273         4548 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_251118, whole genome shotgun sequence.
ACCESSION   DBNHKV010000273 DBNHKV010000000
VERSION     DBNHKV010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_251118"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..646
                     /locus_tag="AC16PP_08615"
     CDS             248..646
                     /locus_tag="AC16PP_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR6481729.1"
                     /translation="MTISITSALDNDQVSLQLLKVLAENSFDSILITDTTKAGRVIYA
                     NKAFKKLAGYGQDEIVGKTPRELQGVGTSKEVIAELRAALSTGRKFEGRAINYKKDGT
                     PFVMHWRVLPVKLGGDIKAWIAIQRESSGS"
     gene            complement(767..1189)
                     /locus_tag="AC16PP_08620"
     CDS             complement(767..1189)
                     /locus_tag="AC16PP_08620"
                     /inference="COORDINATES: protein motif:HMM:NF039286.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481730.1"
                     /translation="MEGSANRDHVLIQRQGGTIELYGSDVHTITWYTGPGVLLSRQEF
                     GETLIVELIANVPVEDGVSKVWHGTMVRAPNNPGTEIVREFRAETDVGALQAFSPDSA
                     VWRTKQPAIHVLQLPTDGPLAGRVAGTVSSTSTRVPWS"
     gene            complement(1156..1674)
                     /locus_tag="AC16PP_08625"
     CDS             complement(1156..1674)
                     /locus_tag="AC16PP_08625"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6481731.1"
                     /translation="MEPRVRQEIYGADPDTWVASPDAPFERATPVDGGYLFSDEWPYS
                     TGTDYCDWVILGGMVTAAGPETSPVPDVRMQDVFVPDYRVYEAPKVIEGVYAAKWRLR
                     HMLNELFERTSAGHPITPTERLRFRRNQVRATDRVFDTYAPLARMAGSAGIQEATGLE
                     RWWRDLQTAITS"
     gene            1812..3074
                     /locus_tag="AC16PP_08630"
     CDS             1812..3074
                     /locus_tag="AC16PP_08630"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HIR6481732.1"
                     /translation="MKRSSSPAEAQASGSCYVPPIELTVGQAQPIAANGGLSYMAFDH
                     DGDAGTGRATEDALRLIREGEGQAVIDLIENAPPGPIQTKWGLGFRRYAECLDFIREA
                     GIEAPEGGLAIPLRYTVHEEPSYSVVSSNALWDDPARTDDANALRKDERDSVRRCLYF
                     PQVLRDARRIGEYYPGLSPSSPECMDRLGVALGHCESHCENFYDAAEVERVFYPEMEK
                     LLLEFFPGATDALVYNHDVFDKSYDGDVVEDQDNKDPGVNTRYANLVHNDLNDNSGRV
                     RCRELLTRNLRNFGRERHYTEAEADAKMSRRFMSINLAKPMDTVRQNPFVLCAWPSFA
                     DQPYINNYRIYDDRVGETTRFTHRPDHEWYWFPHQQPNEVSMLKCYDSVTDGSVSRWS
                     FHTACIDPTAPADAPCRKNVVVRSYVFF"
     gene            complement(3059..3469)
                     /locus_tag="AC16PP_08635"
     CDS             complement(3059..3469)
                     /locus_tag="AC16PP_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481733.1"
                     /translation="MNVNRVAAITSSVLVAVVVVAGLIIAGSPGAQRLHRIDQLRVND
                     LRLISSVLNRYWNQHEALPESLGQLVDERFLDELPRDPTTGDPYGYERTASDAVRLCG
                     MFERASVEESPGDYWYHVAGRYCFTLSFTPQKKT"
     gene            complement(3466..4110)
                     /locus_tag="AC16PP_08640"
     CDS             complement(3466..4110)
                     /locus_tag="AC16PP_08640"
                     /inference="COORDINATES: protein motif:HMM:NF039189.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HIR6481734.1"
                     /translation="MDELIDFIRDALKAGASRRQVEKALRSAGWSNEQIAEGLDHFSE
                     TEFVIPVPRPELQGSARDAFLYLVMFTMLYVSAFNFGALLFDFINLAIPDPLDPLGDG
                     YRHFVRSSIRWETSALMVAFPIFLLIAAHINRAIAGDPSRRVSGVRRWLTYLTLAVMA
                     MILLGDVVALIYAWLNGELSLRMILKVTVVALIPAAIFAYYLWSMRADDEALKR"
BASE COUNT          927 a         1485 c         1352 g          784 t
ORIGIN      
        1 acggtcgacg tgccgaacga agcgatcctg gagggtccat ccacgaaccc ttcggacccg
       61 atatacgtga tgtggacggg taccccgcac gcccagaaca tgctgaagac cgcgcgggac
      121 gctggcaggc aaaaaagcaa acggcaacct gcggcgcagc tcgcatcatc agcgcccgca
      181 atcgctcgtt tcacgaggat ttcacgagct cagtcgtagc tttcgacaat caggcaaggg
      241 gaagaccatg accatttcga tcacgagcgc gctggataac gaccaagtca gcctccaact
      301 cctcaaggtg ctggcagaga acagcttcga ctcgattctg attactgaca ccactaaggc
      361 cggcagggtc atttacgcga acaaagcctt caaaaaactg gcaggttacg ggcaggatga
      421 aatcgtcgga aagacgccgc gagaacttca gggtgtcggc accagcaaag aagttatcgc
      481 cgagctcagg gccgccctct cgacgggtcg gaaatttgag ggcagggcca tcaactacaa
      541 gaaggacggg acgccgttcg tcatgcattg gcgcgtacta ccggtaaaac tcggcggcga
      601 catcaaggct tggatagcca ttcagcgcga gtcctccggc tcctagtcgt cgcacatacc
      661 gggcgctgac gctttgctgg accccttcaa tcgctgacgt cgaggaattc ggctgagggc
      721 gccggcaggc ccgtttcggc gacaacgccg ttgagcgccg cgcgaatcaa ctccacggaa
      781 cccgagtcga cgtagaactg actgtaccag cgacgcgccc tgccaacggc ccgtccgtcg
      841 gcagttgcag cacgtgaatc gccggctgct tggtgcgcca gacggcggaa tccggcgaaa
      901 atgcctgcaa agccccgaca tctgtctccg cccgaaactc gcggacgatc tctgtgcccg
      961 ggttattcgg cgcgcgaacc atcgtgccgt gccacacttt cgaaacgccg tcttccacgg
     1021 gcacgttggc aatcaactca acgatcagtg tttcaccgaa ctcctggcgc gaaagcagga
     1081 ccccgggccc cgtgtaccag gtgatggtgt ggacgtcaga tccgtagagc tcgatggttc
     1141 ctccctggcg ctgtatcagg acgtgatcgc ggtttgcaga tccctccacc atcgctccag
     1201 gcccgtcgct tcctgaattc ctgcggaccc cgccatccgt gccaacggcg cgtaggtgtc
     1261 gaaaacgcga tccgtagctc gaacctggtt ccggcgaaag cgcagccgct cggtcggggt
     1321 aatcgggtgg ccagcggacg tacgttcgaa gagttcgttg agcatatggc gcagcctcca
     1381 tttcgccgca taaacccctt cgatgacctt cggtgcttcg taaacccgat agtcggggac
     1441 gaagacgtcc tgcatgcgca cgtcgggcac gggcgatgtt tcagggcccg ccgccgtgac
     1501 catcccgcca agaatcaccc agtcgcagta atcggtgccg gtggaatacg gccactcgtc
     1561 ggaaaacaga tatccaccat ccactggtgt cgcgcgctcg aatggcgcat cgggcgacgc
     1621 cacccaggta tcgggatcgg cgccgtagat ttcctgtcgc acgcgcggct ccatgaagga
     1681 aatctcccag ggatgcacgc ccacgacgcc ggcgatccat cccgcagcag ctgcgccgcc
     1741 gcgatttggg ctcgattgcc cgcgttggtc ccttgtgcca ggatggtcat tttagcgatc
     1801 ggggccctgt catgaagcga tcgagcagcc cggctgaagc gcaagcgtct ggttcctgct
     1861 acgtcccgcc cattgagctc accgttggcc aggcgcagcc catcgccgcc aacggcggcc
     1921 tgtcgtacat ggccttcgat cacgacggcg acgcgggcac cggcagggcc acggaggatg
     1981 ccctccggct gatccgtgag ggcgagggtc aggccgtcat cgacctgatc gagaacgcgc
     2041 ccccggggcc cattcagacc aagtggggcc tggggtttcg gcgctacgcg gagtgcctcg
     2101 acttcattcg cgaggccggg atcgaggcgc ccgagggcgg cctggccatc cccctgcgct
     2161 acaccgtaca cgaggagccc tcctactcgg tggtgtcctc caacgcgctc tgggatgacc
     2221 cggcacgcac ggacgatgcc aatgctttga gaaaagacga gcgcgacagc gtgcggcgct
     2281 gcctctactt cccccaggtg ctacgcgatg cccgcaggat cggcgagtac tacccaggct
     2341 tatcgccgtc cagccccgag tgcatggatc ggctcggcgt cgcgctcggc cactgtgagt
     2401 cccactgcga gaacttctac gacgcggccg aggtggagcg ggtgttctac ccggagatgg
     2461 aaaagctcct gctcgagttc tttcccggcg ccacggatgc gctggtctac aaccacgacg
     2521 tgttcgacaa atcctacgac ggtgacgtgg tggaggatca ggacaacaag gacccgggcg
     2581 tgaacaccag atacgccaac ctcgtccaca acgatctcaa cgacaacagc ggccgggtgc
     2641 gctgccgaga gctgctcacc agaaacctgc gcaacttcgg ccgcgagcgg cactacacgg
     2701 aagccgaggc ggacgcgaag atgtcgcgcc gattcatgtc catcaacctg gcaaagccca
     2761 tggacacggt acgccagaac ccgttcgttc tgtgcgcgtg gccgtcgttc gccgaccagc
     2821 cctacatcaa caactaccgc atctacgacg accgggtggg cgagaccacg cgcttcacac
     2881 accgtccgga tcacgagtgg tactggtttc ctcaccagca gcccaacgag gtgtccatgc
     2941 tcaagtgcta cgactccgtc accgacggct ccgtgtcgcg ctggtcgttc cacacggcct
     3001 gcatcgaccc gacggcgcct gccgacgcgc cctgccggaa aaacgtggtg gtgcgatcct
     3061 acgtcttttt ctgaggcgtg aagctgagcg tgaagcagta acgcccggca acgtgatacc
     3121 agtaatcgcc cggggactcc tcgacggacg cacgttcgaa catcccgcaa agcctgacgg
     3181 cgtccgaggc tgtccgctcg tacccgtagg gatcgcccgt agtggggtcc cgcggcagct
     3241 cgtcgagaaa gcgttcgtcc accagctggc ccaacgactc cggcagggcc tcgtgctggt
     3301 tccagtaacg atttaggacg ctgctgatca gccgcagatc attgacgcgt agctggtcga
     3361 tccggtgcag gcgctgcgcg ccgggggacc cggcgatgat gagaccggca acgaccacga
     3421 cggccaccag gacggaactc gtgattgcgg ccacgcgatt cacgttcatc gttttagcgc
     3481 ctcgtcgtct gccctcatgg accagaggta gtaggcgaag atggcagccg ggatcagcgc
     3541 caccaccgtg accttcagaa tcatccgcag cgatagctct ccgttcaacc acgcgtagat
     3601 cagcgccacg acgtcgccga gcaggatcat ggccatgacg gcgagcgtga ggtaggtgag
     3661 ccacctgcgc acgcccgaca ctcttcgtga ggggtcgccg gcaatggccc tgttgatgtg
     3721 cgcagcaatc agcagaaaga tcggaaaggc caccatgagc gccgacgtct cccagcggat
     3781 actgctgcga acgaaatgcc ggtaaccgtc accaagaggg tcgagcggat ccgggatggc
     3841 cagattgatg aagtcgaaga gaagcgcgcc gaagttgaac gcgctgacgt agagcatcgt
     3901 gaacatcacc aggtagagaa aggcatcccg cgccgacccc tggagctcgg gcctgggtac
     3961 cggaatcacg aactcggtct ccgagaagtg gtccaggcct tccgcaatct gctcgttcga
     4021 ccaaccggcg ctccgcaggg ccttctccac ctgcctgcgg gatgcgcccg cctttaaggc
     4081 gtcgcgtatg aaatcgatga gctcgtccac gtctcctgcc ctgaccgagt gtcgcccaga
     4141 ccacttcccc actttgctcg gggttgcacc cccggacggc attcgatgcc gttcacctgt
     4201 ttcggcatcc gtatgctgcc agagttcccc ccgtgacaca tcccgtagtt cggctgacgg
     4261 acaggcccgt taacggcgag cccatgcaga tgcgaacgtt cggcctccgc tcgcccggcg
     4321 agccgacgct ccaaaatagt acgagctttc ctccgcccgg gtgtgtacca tgacaagcgc
     4381 ttagaaaccc cgtcccgatc ttctcccgag cttcatttct cgccagtgca tcggcaatcg
     4441 cttctagcct cagacaaccg ctgacaaagg gtcagcggaa aaaaaaccgc gttcgaattg
     4501 attcatatcc gggaatacgc acgcacacgg ctcaacaagc caaggaga
//
LOCUS       DBNHKV010000274         3078 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1383505, whole genome shotgun sequence.
ACCESSION   DBNHKV010000274 DBNHKV010000000
VERSION     DBNHKV010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1383505"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..893
                     /gene="pspA"
                     /locus_tag="AC16PP_08645"
     CDS             153..893
                     /gene="pspA"
                     /locus_tag="AC16PP_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HIR6481735.1"
                     /translation="MSIFSRMSDIINSNINALLDKAEDPEKMVRLIIQEMEEALVEVR
                     STSARAIADKKELARRQEYLDADALEWERKAEVAVAKGRDDLAKGALVERNKAQEAAA
                     AITKDLQILDDTLAKLNDDVTALQAKIKDAKARQNAIIMRGKAAQTRLGVRRQLNDHN
                     IDDAIQRFESYERKMDDLEGQIEAYDMGRKTLADEIAELEVDETLDEELAQLKARMGN
                     ETRSSADAAGAKDAPGREQNCPEHSANG"
     gene            902..1147
                     /gene="pspB"
                     /locus_tag="AC16PP_08650"
     CDS             902..1147
                     /gene="pspB"
                     /locus_tag="AC16PP_08650"
                     /inference="COORDINATES: protein motif:HMM:NF006993.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HIR6481736.1"
                     /translation="MDGLAIAFFVPTVVFLTIVAPIWIFMHYRSKQRAQGALSEDERM
                     ELEALTSQAERMAERIETLESILDSETPGWRSRAQTE"
     gene            1257..1877
                     /locus_tag="AC16PP_08655"
     CDS             1257..1877
                     /locus_tag="AC16PP_08655"
                     /inference="COORDINATES: protein motif:HMM:NF015957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR6481737.1"
                     /translation="MEKLEAALHQLVDTARDQLSDRATAFIEETTATLEREFGRGTGP
                     DRPAAGRRRHGHGHRARDRATEDGIPSRSRRLYRDPGRAKIGGVCAGIARYYGIETWV
                     VRCAAVTGLIFVPQFVFPAYWIAYFLMGLPPREPASGSNGRARDDHSSPAPELGSKLS
                     PRRSLRNIQADLTQIELKLRRMETHVVSGQYELQKELNKIDEVGRG"
     gene            1913..2143
                     /locus_tag="AC16PP_08660"
     CDS             1913..2143
                     /locus_tag="AC16PP_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481738.1"
                     /translation="MDSVFMMVVLIVLISCGAGVANNYLRNQRKRAEADADPEVRTDL
                     DRLRERVEVLEKIVTDEKFHLAKEINRLEREP"
     gene            complement(2118..2816)
                     /locus_tag="AC16PP_08665"
     CDS             complement(2118..2816)
                     /locus_tag="AC16PP_08665"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR6481739.1"
                     /translation="MTFNRHPPLVIGHRGAAGLKPENTLASFSAALELGVDAVELDVH
                     LVEDALVVIHDSTLDRTTNGIGRLSDCTLDTLRLLDAGGGAQIPLLEEVFDLVPPDVG
                     INVELKGVGTGERLADFLTGRWRPEVLVSSFRRPELAAFRRRCPETNVAPLFSRWHPR
                     IWDVAARFTAWSVNLNIKLATRKRIADAQRRGFRVLVYTVNDLDLARSLVDAGVHGIF
                     TDHPERIRVPVLSD"
     gene            2872..>3078
                     /locus_tag="AC16PP_08670"
     CDS             2872..>3078
                     /locus_tag="AC16PP_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6481740.1"
                     /translation="MGYQVLARKFRPAGFDALVGQEHVVRALRHALDNDRLHHAYLFT
                     GTRGVGKTTIARLVAKSLNCEAGVS"
BASE COUNT          640 a          922 c         1010 g          506 t
ORIGIN      
        1 gacattcgcg tcagaaataa tatcaattaa aacatatagt tacgtatacg aaaggttttg
       61 gcacggaact tgagaagagg tatgtgcaac ggcttcacga gattcagcac gtcgacgggg
      121 ccggcacagg aaactacaga ggcagatttg agatgagcat tttttctcgc atgtccgaca
      181 tcatcaattc gaacatcaac gccctgctcg acaaggcgga agatcccgag aagatggtgc
      241 ggctcatcat ccaggaaatg gaggaagcgc tcgtcgaggt gcgcagcacg tcggcccggg
      301 caatcgcaga caagaaggag ctggctcgcc gtcaggagta cctcgatgcg gatgccctcg
      361 agtgggagcg caaggccgag gtagcggtgg cgaagggccg cgatgacctc gccaagggcg
      421 cgctcgtaga gcgcaacaag gcacaggaag cggcggcggc catcacaaag gatctgcaga
      481 tcctcgacga cacgctggca aagctgaacg acgacgtcac cgcgctgcag gcgaagatca
      541 aggacgccaa agctcggcag aacgcgatca tcatgcgcgg caaggcggcg cagacgcgcc
      601 tgggcgtgcg ccgtcagctg aacgaccaca acatcgacga tgcgattcag cgattcgaga
      661 gctacgagcg taagatggac gatctcgagg gtcagatcga ggcctacgac atgggtcgaa
      721 aaacccttgc cgatgagatc gccgagctcg aggtggacga gaccctcgat gaagagctcg
      781 cccagctcaa ggcgcgcatg ggaaatgaaa cccgatcatc cgctgacgcc gcgggagcga
      841 aggacgcgcc cgggcgggaa cagaattgcc cggaacactc tgctaacggt taggagcaac
      901 catggacggc ttagctatcg cgttcttcgt ccccacggtc gtctttctca ccatcgtcgc
      961 gccgatctgg atcttcatgc attaccggag caagcagagg gcccaggggg ccttgtccga
     1021 ggacgagcgc atggagttgg aggctttgac gagccaggcg gagcgaatgg cggagcgtat
     1081 cgaaaccctc gaatcgatcc tcgattccga gacgcctggg tggcggtcgc gggcgcaaac
     1141 ggaataggct gagtggacgg agcgaaggca tgaacgattc gaattcaggt ggtggatcct
     1201 ctcgatcgcc tggcgcaggc cggcagcgcg acggagcctc tgattttcag caggccgtgg
     1261 aaaagctgga agcggcgttg caccagctgg tcgacacggc cagggatcag ctgtcagacc
     1321 gagccactgc cttcatagaa gaaaccacgg ctacgctgga gcgagagttc gggcgcggca
     1381 cagggcccga ccggccggcg gccggaaggc gccgccacgg gcacgggcat cgggcgcgtg
     1441 atcgcgcaac ggaggacggc attccctcgc gatcccgccg gctgtaccgg gatccggggc
     1501 gcgccaagat cggcggcgtg tgcgccggca tcgcgcggta ctacggcatc gagacctggg
     1561 tggtgcgctg cgcggcggtg acggggctca tcttcgtccc ccaattcgtg ttccccgcct
     1621 actggatcgc ctatttcctg atgggtctgc cgccgcgtga gcctgcgtcg ggcagtaacg
     1681 gccgtgcccg cgacgatcat tcttcgccgg ccccggagct tggttcgaag ctgtcgccgc
     1741 gtcgcagcct gcgcaacatc caggcagatc taacgcagat cgagctcaag ctgcgccgca
     1801 tggaaaccca tgtggtttcc ggccagtacg agctgcagaa agaactcaac aagatcgacg
     1861 aggtcggccg tggctgaccc ggccaccgcc aaccctgatc cgggggcctg ctatggatag
     1921 tgtcttcatg atggtggtgc tgatcgtgct gatcagctgc ggagcaggcg tggccaacaa
     1981 ctacctgaga aaccagcgca agcgggcgga ggcagacgcc gaccctgagg tccgcacgga
     2041 cctagaccgt cttcgtgagc gggtcgaggt gctcgagaag atcgtcacag acgagaaatt
     2101 tcacctcgcc aaggaaatca atcgcttaga acgggaaccc tgatccgctc ggggtgatcg
     2161 gtgaagatcc cgtggacgcc tgcgtccacc aggctgcgcg cgagatcgag gtcgttgacc
     2221 gtgtagacga gcacccgaaa accgcgtctc tgcgcgtctg cgatgcgctt gcgcgtcgcc
     2281 agcttgatgt tcaggttgac cgaccaagct gtgaaccttg ccgcgacatc ccagattcgc
     2341 ggatgccatc gactgaacag cggcgccacg ttcgtctcag ggcaacgcct gcgaaaggcc
     2401 gccagttcgg ggcggcggaa tgacgagacc agcacctccg gtcgccaccg gccggtcaga
     2461 aagtcggcca gccgctcgcc ggtccccacc cctttcagct ctacgttgat accgacgtct
     2521 ggcggcacca gatcgaaaac ttcctccagc agaggtatct gcgcgccgcc cccggcgtcg
     2581 aggaggcgta gcgtgtcgag ggtgcagtcg gagagccggc cgataccgtt cgtggtgcga
     2641 tccagggtcg agtcgtggat gaccaccagg gcgtcctcaa ccagatgcac gtcgagttcc
     2701 accgcgtcca cgcccagctc cagcgcggct gagaacgacg caagcgtgtt ttccggcttc
     2761 aggccggcgg cgccgcggtg tccgatcacc agtgggggat gtcggttgaa ggtcacgggc
     2821 gggtccaggg cagtttgggt gtctgtggct tacccactac aatgggcgat catggggtat
     2881 caggtactag cacgcaaatt ccggcctgcc ggcttcgacg cgctcgtagg ccaggagcac
     2941 gtcgtgcgcg ccctgcgaca cgccttggac aacgaccgtc tgcatcacgc ctatctcttt
     3001 accgggactc gtggcgtggg caagacgacc attgcgcgac tggtggccaa gagcctgaac
     3061 tgcgaggccg gcgtttcc
//
LOCUS       DBNHKV010000275         4084 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1740044, whole genome shotgun sequence.
ACCESSION   DBNHKV010000275 DBNHKV010000000
VERSION     DBNHKV010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1740044"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..372)
                     /gene="soxZ"
                     /locus_tag="AC16PP_08675"
     CDS             complement(61..372)
                     /gene="soxZ"
                     /locus_tag="AC16PP_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HIR6481741.1"
                     /translation="MANDVKIRGKITDGVATVKSLMVHPMETGTRRDADGNLIPAHYI
                     EKVAAYKNDQEVLTADWGASISKNPFLSFRVKNAAPGDVIRITWVDNTGATGAGEAVL
                     K"
     gene            complement(397..843)
                     /gene="soxY"
                     /locus_tag="AC16PP_08680"
     CDS             complement(397..843)
                     /gene="soxY"
                     /locus_tag="AC16PP_08680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HIR6481742.1"
                     /translation="MALNRRKWITSMATAGAAVLLPLRLLADTAAAFKAADANTALKQ
                     LFGDLPVEESTAIEFKIPDIAENGAVVPVTVSTDMPGVRNISIVVDENPNPLSASFNI
                     SPEAFADVSTRVKMGNSSVVHAYVRTADKVYTTSKEVKVTIGGCGG"
     gene            complement(859..1212)
                     /gene="soxX"
                     /locus_tag="AC16PP_08685"
     CDS             complement(859..1212)
                     /gene="soxX"
                     /locus_tag="AC16PP_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HIR6481743.1"
                     /translation="MIARAALVLTTALLVVLAPDQAKAEAPDDLERGKAIASDRNAGN
                     CLSCHMMGDGEMPGNSGPPLLQMRLRFPDRAVLRAQIWDATERNPDSVMPPYGRNLIL
                     TEEEIDRVVDYVLSL"
     gene            complement(1209..2147)
                     /locus_tag="AC16PP_08690"
     CDS             complement(1209..2147)
                     /locus_tag="AC16PP_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6481744.1"
                     /translation="MYRFLSAALVVLAAGCGAPQEAAEPSKPTYDDSRVGHFGYGKPA
                     SAEEIAGWDIDIRPDGLGLPQGSGSVEDGEWLYEAQCAECHGSFGEGVGRFPVLSGGK
                     GSLTDPRPSKTVGSYWPYTSTLYDYIRRTMPFTQPESLTADETYAVTAYVLYLNDLVE
                     DDFVLTHENLASVRLPNEGNFVPDPRPDVSNTRCMTDCRDPAAISVLSEVPTYVPASM
                     TMEEIAEAEPAHPGAATYDQYCSICHDVGVAGAPRIGDAAAWSSRLERGMAALEMNAI
                     NGIASEAGVMPAKGGFAHLADDEVNAAVAYMVEGSQ"
     gene            complement(2131..3444)
                     /gene="soxC"
                     /locus_tag="AC16PP_08695"
     CDS             complement(2131..3444)
                     /gene="soxC"
                     /locus_tag="AC16PP_08695"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017134966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HIR6481745.1"
                     /translation="MRQRQLDQIVKSVAESGDKLPREERRRFLRQGLSLAGATAAASF
                     AGGALADSGNLPPNVPSWTRRLGPGVLTNPYGQPSPFESHVQRRTVDWLTADRIAAIS
                     FTPLGDLNGIITPSGVVFERYHAGAPAIDPNQHRLMIHGMVKRPIILTMDDLMRFPSV
                     SMIRFLECPANGGMEWRGAQMDRLQFSHGMLACCEWTGVLLSTLLEEVGLEADASWIL
                     AEGADGAHMSRSIPMEKALDDALIVYAQNGEALRPEQGYPVRLLNPGWEGNTSIKWLR
                     RLEVGDKPWHHREETSKYTDLMADGTAREFTFVQECNSVVTHPCPERPLKGKGKYFIE
                     GLAWSGRGKIRHVDVSVDGGVTWREARFTSQVLPRALTRFGLEWDWDGSPALIQSRAV
                     DETGYVQPTYGQLKAVRGTNSIYHKNAIHTWRLEPNGTVSNVQIS"
     gene            3581..3916
                     /locus_tag="AC16PP_08700"
     CDS             3581..3916
                     /locus_tag="AC16PP_08700"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6481746.1"
                     /translation="MELEVMQENAGRAADLMKLLGHPHRLMILCQLKEGECSVGELSE
                     KIGINQSPLSQHLARMRHEGVVSSRREAQTVYYSLAGPEVAAVISLLYELYCQPANEE
                     MQAPTSAAR"
BASE COUNT          657 a         1298 c         1341 g          788 t
ORIGIN      
        1 caatggccgt gggccgccgc caagcgacgg gcctcggtcc gcgtcggcgt tcaggcgcgc
       61 tcacttgaga accgcttcgc cggccccggt ggctccggtg ttgtccaccc aggtgatgcg
      121 gatgacgtcc ccgggcgccg cgttctttac tctgaaggag aggaagggat tcttggagat
      181 ggaagctccc cagtccgcag tcagcacttc ctgatcgttc ttgtaggcgg cgaccttctc
      241 gatgtagtgg gcggggatca ggttgccgtc ggcgtcccgg cgcgtcccgg tttccatcgg
      301 gtgcaccatg agagacttta cggtcgctac gccgtctgtg atcttgccgc gaatttttac
      361 gtcatttgcc atgtcgtcag tttcctcgct tgtcgctcag ccgccgcagc cgccgatggt
      421 gaccttcacc tctttgctcg tcgtgtagac cttgtccgcg gttcgcacgt aagcgtgaac
      481 gacggagctg tttcccatct tgacccgggt ggatacgtcg gcgaaggctt ccggagatat
      541 gttgaagctg gccgacaacg ggttggggtt ctcgtccacg actatgctga tgttccgaac
      601 gccgggcatg tccgtggata ccgtcacggg gacgacggcg ccgttctcgg cgatgtcggg
      661 gattttgaac tcgatggcgg tactctcttc caccggcaga tcgccgaaga gctgcttgag
      721 cgccgtgttt gcatccgcgg ccttgaaagc ggctgcggta tccgcgagca ggcgaagcgg
      781 cagcagcact gcggcgccgg ccgtggccat gctcgtgatc catttgcgtc gatttaaagc
      841 catagcttcc tcgtggtttt acagtgagag aacgtagtcg accacccggt cgatctcctc
      901 ctcggtcaga atcaggttgc gtccgtaggg cggcatgacc gaatccggat tgcgctcggt
      961 ggcgtcccag atctgcgctc gcagcacggc ccggtccggg aatctcaggc gcatctgcag
     1021 caggggcggg ccgctgttgc ccggcatctc gccgtctccc atcatgtgac aggacaggca
     1081 gttgccggca ttgcgatcag atgcgatggc cttgccccgc tccaggtcgt cgggggcctc
     1141 ggccttggcc tggtccggcg ccaaaacgac cagcagggcg gttgtcagga ccagcgcggc
     1201 tcgtgcaatc attgagaacc ctccaccata taggccaccg ctgcattgac ctcgtcgtcc
     1261 gccaggtgcg cgaagccgcc ttttgccggc atgacgccag cctccgatgc gattccgttg
     1321 atggcgttca tttccagggc cgccatgccg cgctcgaggc gcgatgacca ggcagcggcg
     1381 tcgccgatgc ggggtgcgcc ggcgacaccg acatcgtgac atatgctgca gtactgatcg
     1441 tacgtcgccg cgcccggatg ggccggttcg gcctcggcga tctcctccat ggtcatggag
     1501 gccggcacgt aggttggaac ttcggagagc acgctgatgg ccgcggggtc gcggcaatcg
     1561 gtcatgcagc gcgtgttgct cacgtccggc cgggggtccg gcacgaagtt gccctcgttg
     1621 ggaagacgca cggaggccag attttcgtgg gtgagcacga agtcgtcttc gaccagatca
     1681 ttcaggtaca ggacgtaggc ggtcaccgcg tacgtctcgt cggccgtcag ggactccggc
     1741 tgcgtgaagg gcatggtgcg gcggatgtag tcgtacaggg tgctggtgta gggccagtag
     1801 ctgccaacgg ttttgctggg ccgcgggtct gtcagcgagc cctttccgcc agacagcacg
     1861 gggaagcgcc cgaccccttc gccgaaggat ccgtggcact cggcgcactg cgcctcgtag
     1921 agccactcgc cgtcctcgac ggatccgctc ccctggggaa gtcccaggcc gtccggtctg
     1981 atgtcgatgt cccaccccgc gatttcctcg gcgctcgccg gcttgccgta gccgaagtgg
     2041 ccgacgcggc tgtcgtcgta cgtcggcttg gatggttcgg ccgcctcctg gggggcaccg
     2101 cagccagcgg cgaggacgac gagcgcggca ctaagaaatc tgtacattgc tcaccgtccc
     2161 gttgggttcc agccgccagg tgtgaatggc gttcttgtga tagatggagt tggtgccgcg
     2221 cacggccttg agctgcccgt aggtgggctg aacatagccg gtctcgtcga cggcgcggct
     2281 ctggatcagt gcgggcgagc cgtcccagtc ccactccagg ccgaatcgcg tcagcgccct
     2341 tggcagcacc tggctggtga agcgcgcttc ccgccaggtc acgcctccat ccaccgagac
     2401 gtccacgtgc ctgatcttgc cgcggccgga ccaggcgagc ccttcgatga aatactttcc
     2461 tttgcccttg aggggccttt cggggcaggg atgggtcacg acggagttgc actcctggac
     2521 gaaggtgaac tcgcgtgcgg tgccgtctgc catcaggtcg gtgtacttgg aggtctcttc
     2581 gcggtgatgc cacggtttgt cgccgacctc gagccgtcga agccacttga tcgaggtgtt
     2641 gccctcccat cccggattca gcaggcgcac cggatatccc tgctccggcc gcagggcctc
     2701 gccgttctgg gcgtacacga tgagcgcatc gtccagcgcc ttctccatgg ggatgctgcg
     2761 gctcatgtgg gcgccgtccg cgccttcggc caggatccag ctggcgtcgg cctccagccc
     2821 gacctcctcg agcagggtgg agagcagcac gcccgtccac tcgcagcagg cgagcatgcc
     2881 gtggctgaac tgcagccggt ccatctgggc gcctcgccac tccatgcctc cgttggcggg
     2941 acattcgaga aaccggatca tgctcaccga cgggaagcgc atcagatcat ccatggtcag
     3001 aatgatcggg cgcttgacca tgccgtggat catcaaccgg tgctggttcg ggtcgatggc
     3061 cggcgcgccg gcgtgatagc gctcgaacac cacgccgctc ggcgtgatga tgccgttgag
     3121 atcgcccaga ggcgtgaagc tgatggcggc gattcggtcc gccgtcagcc agtccacggt
     3181 gcgccgctgg acgtgggatt cgaaggggga gggttggccg taggggttgg tcagaacgcc
     3241 cggacccagc cgccgggtcc atgacgggac gtttggagga aggttgcccg aatccgcgag
     3301 cgccccgccg gcgaagctcg cagccgccgt ggctccggcg aggctcaaac cctgccgcag
     3361 gaagcgtctt cgctcctccc gtggcagctt gtcgccggac tcggcaacgg acttcacgat
     3421 ctgatccagc tgacgttgac gcacgctccc tccgcgtata ttttccgtcg cgcgatgcga
     3481 agagttagta tattataaat ttcttaagta aaggcaagcc gaaatatcgg ctggatttgg
     3541 agggtcgaaa aacggcggcc cgccatcgga gtattcgttg atggaactcg aagtcatgca
     3601 ggaaaacgcc ggccgggcgg ccgacctgat gaagctgctc gggcacccgc accggctcat
     3661 gattctctgc cagctcaagg aaggcgaatg ctccgtgggc gagctcagcg agaagatcgg
     3721 catcaatcag tcgccgctgt cccagcatct tgcccgcatg cgccacgagg gcgtggtgag
     3781 cagcaggcgc gaggcgcaga cggtctacta ctcgctcgcc ggcccggaag tcgcagcggt
     3841 gatctcgctg ctctacgagc tctactgcca accggcgaac gaggagatgc aggcgccgac
     3901 ttcggcggcc cgctgagccc cctcgagacg ctttcaggcg tcagccgtcc ttatcacagt
     3961 cggttctacc cggtcagtat tcggatcggt gctcgaagcg cccgcccatg acgcccaccg
     4021 tccacagcag ccccagggtg acggccagca gagcactgcc atcgaaaacg gtgcgccagc
     4081 cgtt
//
LOCUS       DBNHKV010000276         5941 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_463073, whole genome shotgun sequence.
ACCESSION   DBNHKV010000276 DBNHKV010000000
VERSION     DBNHKV010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_463073"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /gene="ftsA"
                     /locus_tag="AC16PP_08705"
     CDS             complement(<1..762)
                     /gene="ftsA"
                     /locus_tag="AC16PP_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR6481747.1"
                     /translation="MASDSELTRIVGLDIGTSKVAAIVAELNLEGELEIIGLGTHPSR
                     GLKKGVVVNIESTVQSIQRAVEEAELMAGCHIENVYAGIAGSHIRSINSHGIVAVRDR
                     EVYPQDVDRVIDAAQAMAIPADQKILHILPQEYVIDNQEGVKEPLGMSGVRLEAKVHL
                     VTCAVNAAQNIEKCIVRCGLTVNDIILEQLASSYSVLTDDERDLGVCLVDIGGGTTDI
                     AVFTGGAIRHTAVIPIAGDQVTNDIAMALRTPTQNA"
     gene            complement(752..1456)
                     /locus_tag="AC16PP_08710"
     CDS             complement(752..1456)
                     /locus_tag="AC16PP_08710"
                     /inference="COORDINATES: protein motif:HMM:NF015739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR6481748.1"
                     /translation="MRRVSAVLLIVLAGAVVAGLVWQMDRPIENVRVIGELTPGEQQQ
                     IEAAVLTGLDAGLLSVDLAEVRDRLRSLSWTRRVTVRRVWPPGLDIAVDKDVPVAVWG
                     DGAHLTSNGRVIAIPDAPAALPVFDCALASPRSALEVYLLLQESLPGAGRRISALRQT
                     SLGEWEVELRGGLRVALGDRDIAQRIARFGIVYERALKDRIETVDYVDARYTGGLAVG
                     WKDPLLAYEEPTANGI"
     gene            complement(1449..2837)
                     /gene="murC"
                     /locus_tag="AC16PP_08715"
     CDS             complement(1449..2837)
                     /gene="murC"
                     /locus_tag="AC16PP_08715"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR6481749.1"
                     /translation="MRRVRRIHFVGIGGAGMCGIAEVLLNQGYQVSGSDLRASETTAR
                     LERLGATVHVGHAAGHVEGKDVVVVSSAVAGDNPELRAAHARRIPVVARAEMLGELMR
                     YRHGIAVAGTHGKTTTTSLITAIFQTAGLDPTFVIGGLLNSTGTNAQLGAGRYLIAEA
                     DESDASFLHLKPMQAVITNIDRDHMGTYHNDFAELRGAFVRFVHRLPFYGSVVLCTDD
                     ANVRDVLPELARPMLTYGFAEDGDYRARDLDVNGASWRFVAERPDGHGDLEISLAVPG
                     EHNVLNALAAAAVATDEGIPDEAIVAGLSQFRGVGRRFEVFAQTPVGCARVTMVDDYG
                     HHPSEVAAVIQTARRVWPDRRLVMVYQPHRFTRTRDLYDDFVRVLSEVDALVLLEVYA
                     AGEEPIPGADGKALCQGLRQRGLVNPTYAADPEEALGLLPDIVRDGDVVLVQGAGSVN
                     QISLSLKEVADA"
     gene            complement(2857..3942)
                     /gene="ftsW"
                     /locus_tag="AC16PP_08720"
     CDS             complement(2857..3942)
                     /gene="ftsW"
                     /locus_tag="AC16PP_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIR6481750.1"
                     /translation="MTARLDMTLLLSWLLILVLGTVMVASAAVAISESLVWKHGIFLL
                     VSLTAFGVVLAVPLAVWQRCHHLAWGLAVILCILVLVPGIGVEVNGARRWIGLGAFTL
                     QASEFAKLLVLVYVAGYLARFDEAVRESSIALLRPLMMVAIVALLLLMEPDFGSVVVL
                     GAAVCGMLFVAGTRLRHFLLLVVVAALLLAALALLQPYRMQRLVSFTDPWSFAFGSGY
                     QLTQALIAFGRGEVTGLGLGEGIQKLFYLPEAHNDFIFAVIAEELGLLGTVAVVVLFG
                     TLIARVLLIAGRAVAEERYFAGYLTYGVGLLLGVQCLVNMGVNTGMLPTKGLTLPFVS
                     FGGNSLLVCSTMLGLAFRAQMEAKGRG"
     gene            complement(3939..5246)
                     /gene="murD"
                     /locus_tag="AC16PP_08725"
     CDS             complement(3939..5246)
                     /gene="murD"
                     /locus_tag="AC16PP_08725"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR6481751.1"
                     /translation="MKRTAIIGLGVTGLSCLEYLHGEDELVVLDTRAEPPHAGVVPDY
                     PDTEFHFGLAGFDFAGVDRVIVSPGVELDSCLVRAARAAGAELASDIDLFCEAAQAPI
                     TAITGTNGKSTVTALMGHLLRATGGNPGVGGNLGEPALSLLDEACDSYVLELSSFQLE
                     RLRPWRFSAATILNVTEDHLDRHRSLAEYAASKQRVYRDCGRAVANRADPLTFPQHDV
                     DELVTFGGDAPGEGDWGVVDGVLARGERAVLPVEALPLSGRHNELNVLAACALAWHPR
                     ADLQSLAGAVSSFRGLPHRCQLVAEAGGVSYVNDSKATNVGATVAALQGLGESSGGSL
                     ILIAGGDGKGADFTALREPVSRYVKTAVLLGVDADAMARALDGAVPLVRVDTLEAAVV
                     AARNAAGPGDLVLLSPACASLDMFENFADRGERFAAAVMEFAA"
     gene            complement(5266..>5941)
                     /gene="mraY"
                     /locus_tag="AC16PP_08730"
     CDS             complement(5266..>5941)
                     /gene="mraY"
                     /locus_tag="AC16PP_08730"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR6481752.1"
                     /translation="QSLVGLGAAAFLYYTAEAPQETELIVPFFKEVAIPLGVGYLAVA
                     YFVIVGTSNAVNLTDGLDGLAILPTVMVGAALGLIAYLVGHTEFSSYLQIPYIADAGE
                     LAVFCGALIGAGLGFLWFNTYPAQVFMGDVGALALGAALGVVAVIVRHEIVLFIMGGL
                     FVLETVSVILQVASYRMTGRRIFRMAPIHHHFELKGWPEPRVIVRFWIVTLVLVLIGL
                     STLKLR"
BASE COUNT         1091 a         2024 c         1928 g          898 t
ORIGIN      
        1 tgcgttctgc gtgggcgttc gcagcgccat ggcaatgtcg ttggtgactt ggtcaccggc
       61 gatggggatc accgccgtgt gacgaatggc gccgcccgtg aacaccgcga tgtccgtggt
      121 gccgccgcct atgtccacca ggcagacgcc caggtcgcgc tcgtcgtccg taagcacgga
      181 gtagctcgag gcaagctgct cgagaatgat gtcgttgacg gtcaggccac accgcacgat
      241 gcacttctcg atgttctgcg cggcgttcac ggcgcaggtg accagatgca ctttggcttc
      301 gagccgcacc cccgacatcc cgaggggctc tttcaccccc tcctggttgt cgatgacgta
      361 ttcctgggga aggatgtgca gaatcttctg gtccgccggg atggccatgg cctgggccgc
      421 atcgatcacc cggtccacgt cctggggata gacctcgcgg tcccgcacgg cgacgatccc
      481 gtgggagttg atgcttcgta tgtgactgcc ggcgatgccc gcgtagacgt tctcgatgtg
      541 gcagccggcc atgagctcgg cctcctccac ggcgcgctgg atggactgca cggtggattc
      601 gatgttcacc accacgcctt tcttcaatcc ccgtgatggg tgcgttccga gcccgatgat
      661 ctcgagctcc ccttcgaggt tgagctcggc cacgatggcg gccaccttgc tcgtgccgat
      721 gtcgagaccg acgattcggg tcagctcact gtcagatgcc atttgctgtg ggttcctcgt
      781 atgcaaggag cggatctttc caaccaaccg cgagaccgcc ggtataccgc gcatcgacgt
      841 agtcgacggt ctcgatgcgg tccttcaacg cccgttcgta gacgatgccg aatcgggcga
      901 ttcgctgggc gatatcccgg tcgcccagcg caacccgaag accgccgcga agctcgactt
      961 cccactcgcc gagggacgtc tgcctgagcg ccgagatgcg tcttcccgcg cccggcaggg
     1021 actcctgcag cagcagatag acttccagcg cgctgcgcgg gctggccagc gcgcagtcga
     1081 agacgggcag cgcggcgggc gcgtccggga tcgcgatcac ccggccgttg ctggtgaggt
     1141 gagcaccgtc gccccagacc gcgacaggaa catccttgtc gacggcaatg tcgagccccg
     1201 gaggccatac gcggcggacc gtcacgcgac gcgtccagga aaggctccgc agccggtcac
     1261 gcacctcggc gagatccacg ctcagcaggc ccgcatcgag gccggtgagc accgccgcct
     1321 cgatctgctg ctgctccccg ggggtgagct cgccgatgac gcgcacgttc tcgatgggcc
     1381 gatccatttg ccacaccagc ccagccacca cggccccggc cagcacaatc aggagcacgg
     1441 cggacaccct acgcatccgc cacctccttg aggctcagcg agatctggtt gacgctgccg
     1501 gccccctgaa cgagcaccac gtcgccatcc cggacgatgt cgggcaggag ccccagggct
     1561 tcctcggggt cggcggcgta ggtggggttg acgagcccgc gctgccgcag gccctggcac
     1621 aacgccttgc cgtccgcgcc aggtatgggt tcctcgcccg ccgcgtaaac ctccagcagc
     1681 acgagggcgt ccacctcgga gagaactctc acgaaatcgt cgtaaagatc cctggtacgg
     1741 gtgaaccggt gcggctgata gaccatgacg agccgccgat cgggccagac gcggcgcgcg
     1801 gtctgaatca ctgcggccac ttcggaagga tggtggccgt agtcgtcgac catcgtgacg
     1861 cgggcgcagc ccaccggcgt ctgcgcgaaa acctcgaacc gccggcccac gccgcggaac
     1921 tgcgacaggc cggcgacgat ggcctcgtcc ggtatgcctt cgtcggtggc gaccgctgcc
     1981 gccgcgagcg cgttgagcac gttgtgctcg ccgggaacgg ccagggagat ctccaggtca
     2041 ccgtgaccgt cgggacgctc ggcgacgaaa cgccacgatg cgccgttcac gtcgagatcc
     2101 cgggcgcggt agtcgccgtc ctccgcgaag ccgtaagtca gcatgggccg ggcaagctcg
     2161 ggcagaacgt cgcgcacgtt cgcatcgtcc gtgcacagca cgacgcttcc gtagaagggc
     2221 agacggtgca cgaaccgcac gaacgcgccg cgcagctcgg caaagtcgtt gtggtaggtg
     2281 cccatgtgat cccggtcgat gttggtgatc acggcctgca tgggtttgag gtgaagaaac
     2341 gacgcgtcgc tttcgtcggc ttccgcaatc aggtaccggc cggcgcccag ctgcgcattg
     2401 gttcccgtgc tgttgagcaa accgccgatg acgaaggtgg ggtccaggcc cgcggtctgg
     2461 aatatggccg tgatcaggct ggtagtggtc gtcttgccgt gggttccggc gacggcaatg
     2521 ccgtgacgat agcgcatgag ctccccgagc atctccgccc gcgccacgac cggtatccgc
     2581 cgcgcgtgag ccgcgcgaag ctcagggttg tctcccgcga ccgcgctgga taccacgacc
     2641 acgtccttgc cctcgacgtg ccccgcagca tggcccacat ggacggttgc gccgagccgt
     2701 tccagtcgcg ccgtggtctc cgacgcgcga aggtctgatc ccgacacctg gtatccctgg
     2761 ttgagaagca cctcggcaat gccgcacatg ccggcaccgc caatgcccac gaagtgaatg
     2821 cgccgcaccc ggcgcatctc cggcacgtag tacgactcag ccacggcctt tcgcctccat
     2881 ctgggcccgg aacgcgagcc ccagcatggt gctgcacacc agcaggctgt taccaccgaa
     2941 gctcacgaat ggcagcgtga ggcctttggt cggcaacatc ccggtgttga cgcccatatt
     3001 gacaaggcac tgcacgccca acagcaggcc gacgccgtag gtgaggtagc cggcgaagta
     3061 tctttcttcc gccaccgccc gaccggctat gagcagcacc ctggcgatca gcgtgccgaa
     3121 cagaacgacc accgcgacgg tgcccagcag gccgagctcc tcggcaatca cggcgaatat
     3181 gaagtcgttg tgcgcttccg gcaggtagaa gagtttctgg ataccctcgc caagaccgag
     3241 cccggtgacc tctccacgcc cgaaggcgat cagcgcctgg gtgagctggt agccgctgcc
     3301 gaaggcgaac gaccacgggt ccgtgaagct caccagcctc tgcatgcggt agggctgcag
     3361 cagcgcaagg gcggccagca gcagagccgc gaccacgacg agcaggagaa agtgacgaag
     3421 ccgggtgccc gcgacgaaga gcatcccgca gacggcggcg ccgagaacca cgacggagcc
     3481 gaagtcgggc tccatgagca gcagcagcgc gacgatggcc accatcatga gcggccgcag
     3541 cagcgcgatg gagctctccc gcaccgcttc gtcaaagcgg gccagatagc cggccacgta
     3601 gaccagcacc aggagcttgg cgaactctga cgcctgcagc gtgaaggcgc caagccctat
     3661 ccagcgccgc gcgccgttca cctcgacgcc gatgccggga accagcacca ggatgcagag
     3721 aatcaccgcg aggccccagg cgaggtggtg gcaacgctgc caaacggcga gcggcaccgc
     3781 gagcacgacg ccgaacgcgg tcaggctgac gagcaggaag atgccgtgct tccagaccag
     3841 gctttcactg atggcgacgg cggcggaggc caccatgacc gtgcccagca ctaggatgag
     3901 cagccaggac agcaggagcg tcatgtcgag cctcgccgtc atgccgcgaa ctccatgacc
     3961 gccgccgcga agcgctcacc gcgatcggcg aagttctcga acatgtccag gctggcgcag
     4021 gcgggggaaa gcaggaccag atcgccggga cccgccgcgt tgcgggccgc gaccaccgcg
     4081 gcctccaggg tatccactct gacgagcgga acggcgccgt ccagagcccg agccatggcg
     4141 tcggcatcca cgccgagcag aacggccgtc ttcacgtagc gcgagaccgg ctcccgcaga
     4201 gcggtgaaat ccgcaccctt gccgtccccg cccgcaatca gtatcagcga tcccccggag
     4261 gactctccca ggccctgcag cgccgcgacg gtggcgccga cgttcgtcgc cttggagtcg
     4321 ttcacgtagc tcacgccacc ggcctcggcg accagctggc agcggtgcgg cagcccgcga
     4381 aagctcgata ccgctcccgc cagcgattga agatctgcac gcgggtgcca ggcgagcgca
     4441 caggcagcga gcacgttcaa ctcgttgtgc cggccggaca gcggaagcgc ttccaccggc
     4501 agcacagcgc gctcgccacg ggcgagcact ccgtcgacga cgccccaatc accctcgccc
     4561 ggcgcatcgc cgccgaaggt gaccagctca tccacgtcgt gctgaggaaa ggtgaggggg
     4621 tccgcccggt tggctacggc ccgcccgcaa tcccgataga cgcgctgctt gctggccgcg
     4681 tactcggcaa ggctgcggtg tcggtccaga tggtcttcgg tgacgttgag tatggtcgct
     4741 gcgctgaacc gccaggggcg aagacgctcc agctggaagc tggagagctc gagcacgtag
     4801 ctgtcgcagg cctcgtcgag aagggaaagc gccggctcac cgagattgcc gccgacgccc
     4861 ggattgccgc ccgtggcacg caggaggtgg cccatcagcg ctgtgaccgt gctcttgccg
     4921 ttggttcccg tgatcgcggt gatgggcgct tgcgccgctt cgcagaagag gtcgatgtcg
     4981 ctggcgagtt ccgcgccggc cgcgcgcgcc gctctgacca ggcagctgtc cagctccacc
     5041 ccagggctga cgatcacgcg gtcgacgccc gcgaagtcaa agcccgccag gccgaagtga
     5101 aactccgtgt ccggatagtc gggcacgacg ccggcatggg gcggttcggc acgggtatcg
     5161 agcaccacca gctcgtcctc gccgtggagg tactccaggc aggagaggcc ggttacgcct
     5221 aagccgatga tcgctgtcct tttcaatctg gctgatccgt gtccgctacc tgagcttgag
     5281 cgtggaaagt ccgatcagaa cgaggaccag ggtgacgatc cagaaccgaa cgatcacgcg
     5341 cggctcgggc catcccttga gctcgaaatg atgatgaatt ggcgccatcc ggaatatccg
     5401 acggccggtc atgcggtacg aggccacctg aagaatgacg gagaccgtct ccagcacgaa
     5461 caacccgccc atgatgaaca gcacgatctc gtgacgcacg atgacggcga ccacaccgag
     5521 cgccgcaccc agcgccagcg cacccacgtc gcccatgaac acctgggccg gatacgtgtt
     5581 gaaccagaga aagccgagcc cggcgccgat cagcgcgccg cagaacacgg ccagctcccc
     5641 ggcgtcggca atgtagggta tctgcagata gctggaaaac tccgtgtgac cgaccaggta
     5701 ggcaatcagg cccagggcag cgcccaccat gaccgtgggc agaatggcaa ggccgtcgag
     5761 cccgtcggtg aggttgaccg cgttgctggt tcccacgatg acgaagtagg cgaccgccag
     5821 gtagcccaca cccaatggga tggcgacctc cttgaaaaac gggacgatca gctcggtctc
     5881 ctgaggagcc tccgcggtgt agtagagaaa ggccgcggcc cccagcccca ccaaggactg
     5941 c
//
LOCUS       DBNHKV010000277        11617 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1456413, whole genome shotgun sequence.
ACCESSION   DBNHKV010000277 DBNHKV010000000
VERSION     DBNHKV010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11617)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11617)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11617
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1456413"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="AC16PP_08735"
     CDS             <1..480
                     /locus_tag="AC16PP_08735"
                     /inference="COORDINATES: protein motif:HMM:NF025806.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR6481753.1"
                     /translation="TQNAEEIKIRYACALTQLAGPDETIKVPGVGDKPAKELSRQALA
                     EVVEPRYDELFTLIQAELRRSGFEDLIAAGIVLTGGASKMEGVVELAEEIFHMPVSLG
                     APRDVAGLKDIVRNPVYATGVGLLQYGREREREMPGRGRLRGKGAFGRMKKWLSDNF"
     gene            494..1642
                     /gene="ftsZ"
                     /locus_tag="AC16PP_08740"
     CDS             494..1642
                     /gene="ftsZ"
                     /locus_tag="AC16PP_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR6481754.1"
                     /translation="MFELVDSAPQSAVIKVVGVGGGGGNAVQHMLSRQVEGVDFIAAN
                     TDAQALKHLDAKTILQIGSNITKGLGAGANPDIGRQAAIEDRDRLGEVLVGADMVFIT
                     AGMGGGTGTGAAPVVAEVARELNILTVAVVTRPFKFEKRSDVADRGIAELRETVDSLI
                     TIPNEKLLAVLGERTTMLDAFGAANDVLLGAVQGIADLIIRPGIINVDFADVRTVMSE
                     MGMAMMGTGRGTGDNRARDAAEAAIRSPLLEDVDLHGARGILVNVSAGPDLTIGEYSD
                     VGDIINEFASEQATVVVGTVIDQDLGDELKVTVVATGLGEPARPNVVIDNTVIEEVIQ
                     GQIDYRDLDQPTYKRHNQALHKDNLAEDLDTQDLDYLDIPAFLRRQAD"
     gene            1845..2759
                     /gene="lpxC"
                     /locus_tag="AC16PP_08745"
     CDS             1845..2759
                     /gene="lpxC"
                     /locus_tag="AC16PP_08745"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HIR6481755.1"
                     /translation="MALNQKTLKNSIRATGVGLHTGDKVFLTLRPAPVDTGIVFVRTD
                     LDGEPRLPARVENVGDTTMATSLTNGTVSVSTVEHLMSAFAGLGIDNAFVDVSAPELP
                     IMDGSAAPFVFLIQSAGVEEQTAPRRFVRIKKAVAFRQGDVVAELKPFDGFRVSYTLV
                     YDHPVFRKHERSATVDLSNTAYVKEVSRARTFGFLADYEQLRSMNLARGGSLDNAVVV
                     DDYRILNGEGLRQADEFVKHKILDAVGDLYLLGCGLIGEFCGYKSGHGSNNALLRRLL
                     ADPEAFEIVTFANEADAPTGFAAPVVAN"
     gene            complement(2860..3168)
                     /locus_tag="AC16PP_08750"
     CDS             complement(2860..3168)
                     /locus_tag="AC16PP_08750"
                     /inference="COORDINATES: protein motif:HMM:NF017103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HIR6481756.1"
                     /translation="MANLKVDLLLSESQRRFTPLQRLLRQSANQESWTAGLLALLPAS
                     LQNDCRVSDIRNGSIIVVCRNAATATRLRFLAPELLSKLHDLADFRAAQRIEVRVSEF
                     "
     gene            3282..4190
                     /locus_tag="AC16PP_08755"
     CDS             3282..4190
                     /locus_tag="AC16PP_08755"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR6481757.1"
                     /translation="MKIILLRSSGGAHALRVPRWMGALALAIGLVATAAGAVLGMNLL
                     GSHQMAPETVVHWRQEVEAQRLELQALLDRSTAETQAVGRQLAQMQARLLRMEALGAR
                     VAEVADLDDGEFSFDLPVPVGGPDTTTSEPMPMGDLRADVDALARNLKIRETELEILE
                     SMLRDRDYRAEVAVAGRPVTWGWISSSYGERVDPFSGKMAWHAGLDFAGKQGSDVIAV
                     ASGVVTHAGNRFGYGEMVEVTHGDGYVTRYGHHAEIVVAVGEIVKKGQVVGKMGSSGR
                     STGPHVHFEVLKNGRRVDPARYVVRR"
     gene            4373..7096
                     /gene="secA"
                     /locus_tag="AC16PP_08760"
     CDS             4373..7096
                     /gene="secA"
                     /locus_tag="AC16PP_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR6481758.1"
                     /translation="MLKKIFGTKNARELKRMGRIVARINALEADLLEISDDELKATTG
                     RLKERLEQGASLEDLLPEAFAAVRESARRAKDMRHFDVQLIGGITLHEGRIAEMRTGE
                     GKTLVATLAAYLNALAGRGVHVVTVNDYLARRDAEWMGPIYETLGLTVGVIQSGQAPE
                     EKREAYQSDVTYGTNNEFGFDYLRDNMAFSLADKFQRDLGYAIVDEVDSILIDEARTP
                     LIISGPAEQSTELYQKIDRIAPKLKQQPPVEDPEAEPTGDFVVDEKNRQVELTEVGHE
                     KVEAELMRLGLLEEGDSLYAAANLNLLHHVHAALKAHALFKRDVDYMVANGEVVIVDE
                     HTGRAMPGRRWSEGIHQAIEAKERVAIQNETQTLASTTFQNYFRLYDKLAGMTGTADT
                     EAFEFRQIYGLDVVVIPTHMPMVREDNNDLVYLTIEEKYDAIVEDINDCIERGQPVLV
                     GTASIESSERLSGELDRRKIAHNVLNAKQHEREADIIAQAGSPGAVTIATNMAGRGTD
                     IVLGGNWEAEAAQLDSPTDEELQRLRGEWQKRHDAVIAAGGLHIIGTERHESRRIDNQ
                     LRGRSGRQGDPGSSRFYLSMEDNLMRIFASDRIRGIMQSIGLEKGEAIEHRMVNNAIE
                     KAQRKVEGHNFDIRKNLLEFDDVANDQRQVIYQQRRELMAADDIAADIEGLREEVVLD
                     LIAEYIPPQSIEEQWDVDGLEQALQTEFASRQPVRGWLDEDDALNEDSLSRKILAEVE
                     AEYRAKEQDWASHGIDMRVVEKQIMLQVLDQRWKEHLAAMDYLRQSIHLRAYAQKQPK
                     QEYKRESFQLFQELLENIKRDVIRLLCRVQIETSDELEEAERRRREEAERRMRYTHAE
                     SSGLDGGEQEQPDDGAGKPETFVRSERKVGRNEPCPCGSGKKYKQCHGKAA"
     gene            7110..8330
                     /gene="argJ"
                     /locus_tag="AC16PP_08765"
     CDS             7110..8330
                     /gene="argJ"
                     /locus_tag="AC16PP_08765"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019471521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR6481759.1"
                     /translation="MAVNVPPLQELLVVPGVSLGTAGAAIKRQGRDDIAVLLLHPQTR
                     AAGVFTRSSFRAAPVTLADRRLSGGRARALVINSGNANAATGDPGLADAVAVCDAVAA
                     ALDVPGDSVLPFSTGVIGERLPVSRMLPAVVSACGSLAPDGWEAAARAIMTTDTAPKA
                     VSRRVRVAGEEVVITGMAKGAGMIKPDMATMLGFVCCDAAVADGCLPAMVREVAGRSF
                     NRITVDGDTSTNDAFVVCATGCAENAPVTDVGSEGYAELRDGLAAVATELAQSIVRDG
                     EGATRFVTVRVSGGESAAECLKVAYTVAESPLVKTAIFAGDPNWGRFCMAIGRAGIAD
                     LDTGRVAVHLDDVPIALGGLVAPQYEEAAAARVMARQEFEVHVDLGRGSAEETVWTTD
                     LSYEYVRINAEYRT"
     gene            complement(8358..8570)
                     /gene="yacG"
                     /locus_tag="AC16PP_08770"
     CDS             complement(8358..8570)
                     /gene="yacG"
                     /locus_tag="AC16PP_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HIR6481760.1"
                     /translation="MTASEPDRTARIVTCPTCGGRVKWTSGNEFRPFCSERCKLMDLG
                     AWASEQHRIPGNEVDDDLLSGDLDPD"
     gene            complement(8567..9166)
                     /gene="coaE"
                     /locus_tag="AC16PP_08775"
     CDS             complement(8567..9166)
                     /gene="coaE"
                     /locus_tag="AC16PP_08775"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003302803.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR6481761.1"
                     /translation="MSRLVIGITGGIGSGKTAVSDRFKALGVTVVDADIASRVIVEPG
                     RPALVAIAEHFGCDVIQADGSLDRAELRRRVFADPDERRWLEQLTHPLINEHIREELE
                     QAESPYAVLVSPLLAETGQSRFCHRILVVDVPEEVQVRRTMARDANDEAQVRKIIEAQ
                     ASREQRLALADDVIVNDSSLEVLDEEVARLHQVYTEMAQ"
     gene            complement(9196..10050)
                     /locus_tag="AC16PP_08780"
     CDS             complement(9196..10050)
                     /locus_tag="AC16PP_08780"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR6481762.1"
                     /translation="MGETLLAYGWAGLTFAVWVGLSVGSFLNVVIYRLPIMLSRQWEA
                     QARELLEEEPAETGQERFNLMVPRSRCPACQQPISAWQNVPVFSWLFLKGRCRSCGVR
                     ISIRYPMVEVLTGAATITVLALFGYTWLGLAAAVYTWAMIALTFIDYDTQLLPDQITL
                     PLLWLGLITCHFGGVTDLGSGVLGAVIGYLFLWTTFWAFKLITGKEGMGYGDFKLFAA
                     IGAWLGWQVLPGTILIAATLGLAYALAITLAGKREAAQPIPFGPFLAVAGWVSLMARD
                     NVLTLFAA"
     gene            complement(10100..11314)
                     /locus_tag="AC16PP_08785"
     CDS             complement(10100..11314)
                     /locus_tag="AC16PP_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR6481763.1"
                     /translation="MAESAIFLWEGIDKGGRKTKGEISSSTPAIAKAELRRQGINATK
                     VKKKSKAFSLGGGGSVKPVDIALFTRQMSTMMRAGVPLVQAFEIVAGGVDKPKVATLI
                     RALRDDVAGGSSFTAALRKHPLHFDDLFCNLVGAGEQSGALETMLDRIATYKEKTESL
                     KAKVRKAMTYPAAVLVVAFIVSGILLIKVVPQFEEVFAGFGAELPAFTQFVINISEFT
                     QQWWFLIVVAIGGTIATFMAARKRSKKLREAIDRIALKAPIFGNIIEKSAVARYARTL
                     ATTFAAGVPLVEALNSVAGSTGNSVYTKAVYQIRDDVSTGQQLNFSMRSTGVFPNMVV
                     QMVAIGEEAGALDEMLDKSASYYEEQVDNAVDNLTALMEPMIMAVLGVLVGGLIIAMY
                     LPIFQLGRVVGG"
     gene            complement(11318..>11617)
                     /locus_tag="AC16PP_08790"
     CDS             complement(11318..>11617)
                     /locus_tag="AC16PP_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HIR6481764.1"
                     /translation="KETLKAEGFTDEQIDEGFELFEPVGCDSCNNGYKGRVGIYEVVK
                     ITPEMSRIIMEDGNSIKLAETASKLGFKNLRQSGLLKVIQGVTSLAEANRMTTGH"
BASE COUNT         2167 a         3520 c         3879 g         2051 t
ORIGIN      
        1 acgcagaacg cagaggagat caagatcaga tatgcctgcg cgctgacgca gctcgccggt
       61 cccgatgaaa ccatcaaggt tcccggggtc ggcgacaagc cagccaaaga gctgtcgcgc
      121 caggcgcttg cggaggtggt ggagccccgc tacgacgagc tgttcacgct gatacaggcg
      181 gagctgcggc gtagcgggtt cgaagacctc atcgccgcgg gcatcgtgct caccggggga
      241 gcgtcgaaga tggagggggt tgtcgagctt gcggaagaga tcttccacat gccggtcagc
      301 ctgggcgcgc cacgtgacgt ggcgggtctg aaggacattg tgagaaatcc cgtatacgcg
      361 accggcgtcg gcctgttgca gtacggcagg gagagggagc gggagatgcc cggtcgcgga
      421 aggcttcggg gcaagggtgc tttcggccga atgaaaaagt ggttgagcga caatttctag
      481 ttaggagatc aggatgtttg aactggtaga cagtgccccg cagagcgcgg tgatcaaggt
      541 cgtcggagtg ggaggcggcg gtggcaacgc cgtccagcac atgttgagca ggcaggtaga
      601 gggggtggat ttcatcgccg ccaataccga tgcccaggct ctgaagcacc tcgacgcgaa
      661 gacaattctg cagatcggga gcaatatcac caaaggcctc ggcgccggcg cgaatcccga
      721 catcgggcgg caggccgcca tcgaggatcg ggatcgtctg ggtgaggtgc tggtcggggc
      781 ggacatggtg ttcatcaccg ccgggatggg aggcggcacg ggaaccggag ctgcgcccgt
      841 ggtcgcggaa gtggcccgtg agctgaacat cctgaccgtc gcggtggtca cccggccttt
      901 caagttcgaa aagcgctcgg acgtcgccga tcgcgggatc gcggagctgc gtgagaccgt
      961 cgattccctg atcaccatac ccaacgaaaa gctccttgcc gtgctcgggg agcgaaccac
     1021 catgctggat gcgttcggtg cggccaacga cgtgctgctc ggggccgtgc aggggattgc
     1081 cgacctcatc atccggccgg gaatcatcaa cgtggatttt gccgacgtgc ggacggtcat
     1141 gtcggagatg ggcatggcca tgatgggcac gggccgggga acgggagaca atcgcgcgcg
     1201 cgacgcggcg gaagcggcaa tccgctcgcc tctgctcgag gacgtggatc tgcacggcgc
     1261 gagaggcatt ctggtcaacg tctctgccgg gccggatctc accatcggcg agtactccga
     1321 cgtgggcgac atcatcaacg agtttgcctc cgagcaggcc accgtcgtgg tggggacggt
     1381 cattgatcag gatctgggcg acgagctgaa ggtcaccgtg gtggccacgg ggctcggcga
     1441 gccggcaaga cccaatgtgg tcatcgacaa cacggtgatc gaggaggtca tccaggggca
     1501 gatcgactac cgggatctgg accagccgac ttacaagcgt cacaaccagg ctctgcataa
     1561 ggacaacctt gcagaggatc tggacactca ggatctggac tatctggaca ttccggcttt
     1621 cctgcgtcgc caggccgact gaacgtgccc gggtgaaacg tgtgacttac cggctgccgg
     1681 ctgccataat ttagcgccgg ccggcagatg atccccttct gccgggtgcc ggtcggtgca
     1741 caggctcgaa agggcgtgtg cgcggggagc gctttgcgta aaagaggaag aaggagcgcg
     1801 attcgcggct tcttgcaggg gccctggcct gaaggacggg caacatggcg ctgaatcaga
     1861 aaacgctcaa gaactcgatc cgcgccaccg gcgttggtct gcatacggga gacaaggtct
     1921 ttctgaccct gcgcccggcc cccgtggata cgggaatcgt ctttgtgcgc accgatctcg
     1981 acggcgagcc gaggctcccg gcgcgggtgg agaacgtggg cgataccacc atggcgacgt
     2041 cgctgaccaa cggcaccgtc agcgtttcca cggtcgagca tctcatgtcg gcgtttgccg
     2101 ggctcgggat cgacaatgcc ttcgtggacg tcagcgcccc ggagttgccg atcatggacg
     2161 gcagcgccgc gccgttcgtg tttctcatcc agtccgccgg ggtagaggaa cagaccgcgc
     2221 ccagacgatt cgttcgcata aagaaggcgg tggcgttccg gcagggagac gttgtcgcgg
     2281 agctcaagcc gttcgacggg ttccgcgttt cctacaccct ggtctacgac cacccggtat
     2341 tccgcaagca cgaacgctca gccacggtgg atctttccaa cactgcctac gtcaaggagg
     2401 tgtcgcgggc gcgtacgttc ggttttctgg ccgattacga acagctgcga tccatgaacc
     2461 tcgcgcgggg agggagcctc gacaatgcgg tggtggtgga cgactaccgc atcctcaacg
     2521 gggaggggct ccggcaggcc gacgagttcg tcaagcacaa gattctcgac gccgtgggcg
     2581 acctctacct gctcggctgt ggcctgatcg gcgagttctg cggctacaaa tcaggccatg
     2641 gatccaacaa tgcgcttttg cgcaggttac tagctgaccc cgaggctttc gagatcgtta
     2701 ccttcgcaaa cgaagcggac gcgccgacgg gcttcgccgc tcccgtggtt gctaattgat
     2761 tcgcagactc attggccaat ttgaccgaca tgtctcaaaa tgaggctggg gtcgagggga
     2821 taatcgtcac agccgagggc acaaggaacc gggcgcctgt cagaattcgc tgacgcgcac
     2881 ctcgattcgc tgagccgctc ggaaatcggc tagatcgtga agtttgctca gcagttcagg
     2941 cgcaaggaat cgcaaccgcg tagcggttgc agcgtttcgg cagaccacga tgatcgagcc
     3001 gttgcggatg tcgctgacgc gacagtcgtt ctgcagcgaa gcgggcagca gggctaacag
     3061 cccggccgtc caggactctt gattcgccga ctgacgtagg agacgttgga gaggagtaaa
     3121 tcggcgttgg ctttcgctca acagaagatc gactttgaga ttcgccatgg ccgggtctcg
     3181 atcatgaacg accgtgtcgg ccgccctcgg gctatggatt ttggcccggg gcggttcgtt
     3241 tcgccaccgt ttcgggttgc ggtcaggggg gtatcgcgac ggtgaagatc atattgctga
     3301 gaagctcggg tggtgctcat gcgctgcggg tgccccgctg gatgggggcg ctggcgcttg
     3361 ccatcggcct cgtagcgacc gctgcgggcg ccgtgcttgg gatgaacctc ctcgggagcc
     3421 atcagatggc cccggaaacc gtcgtccatt ggcgccagga ggtcgaggct cagcgcctcg
     3481 agctgcaggc cctgctcgat cgatccaccg cggaaaccca ggccgtcggc cgacagcttg
     3541 cccagatgca ggcacggctt ctgcgcatgg aggcgctcgg agcccgcgtg gcggaagtgg
     3601 ccgacctgga cgacggcgag ttctcgttcg atctgcccgt tcccgttggt ggcccggaca
     3661 cgaccacttc ggagccgatg ccgatggggg atctgcgcgc cgacgtggac gctctggcaa
     3721 ggaatctgaa gattcgcgaa acggagctgg agattcttga atccatgctg cgcgaccgcg
     3781 actaccgggc tgaggtggcc gtggccggcc gcccggtgac gtggggctgg atctcctcct
     3841 cgtacggcga gcgggtggac ccgttcagcg gcaagatggc ctggcacgcg ggattggact
     3901 tcgccggcaa acagggttct gacgtcatcg cggtggccag cggcgtggtt acccatgcgg
     3961 gcaatcgctt cgggtacgga gagatggtcg aggtcaccca cggcgacggg tacgtcaccc
     4021 gatacggcca ccacgcggaa atcgttgttg ccgtcggcga gatcgtcaag aaaggccagg
     4081 tcgttggaaa gatgggctcc agcggccgct ccactggccc gcacgtgcat ttcgaggttt
     4141 tgaagaacgg ccgtcgagtc gatccagccc gttacgtcgt acgtcgctga cgcgatccgg
     4201 cagccccgat ctggtctcga cacgcgccgg caggcccgga acccagccga ccttcgctgc
     4261 cttgtcgggc aggctccatc cttcatcgga tgaagcgatc gtctcccgcg gattgtggtc
     4321 gaattagggt aaactgccgc cggtttcgca tccggcaccc aggcgaaagc acatgctcaa
     4381 gaaaattttc ggcaccaaga acgcgcgaga gctcaagcgg atgggcagga tcgtggcccg
     4441 gatcaacgcg ctcgaggccg acctgctcga gatttccgac gacgaactca aggccaccac
     4501 cggccggctg aaggaacggc tggagcaggg cgccagtctg gaggacctgc tcccggaagc
     4561 gtttgccgcg gtgcgtgagt ctgcccgccg cgccaaggac atgcgccatt tcgacgtcca
     4621 gctgattggt gggatcaccc tccacgaggg gcgcatcgcg gagatgcgca ccggcgaggg
     4681 caagaccctg gtggcgacgc tggccgccta cctcaacgcc ctggccggtc gcggggtgca
     4741 cgtggtgacc gtcaacgact accttgcacg acgcgacgcg gagtggatgg gaccgatcta
     4801 cgagacgctc ggcctcacgg tgggcgtgat ccagtcgggt caggcgccgg aagagaagcg
     4861 ggaagcctat cagtcggacg tgacctacgg cacgaacaac gagttcggct tcgattacct
     4921 gcgcgacaac atggcgttct cgctggccga caagtttcag cgtgacctcg gttacgccat
     4981 tgtcgacgag gtggactcca ttctcatcga cgaggcccgc acgccgctca tcatctccgg
     5041 ccccgccgag cagagcacgg agctttatca gaagatcgac cggatcgcgc ccaagctcaa
     5101 gcagcaaccg ccggtcgagg atccggaagc cgagccgacg ggcgatttcg tggtggacga
     5161 gaaaaaccgc caggtggagc tgaccgaggt cggccatgag aaggtggagg cggagctcat
     5221 gcggctcggc ctcctcgagg aaggcgacag cctttacgcg gccgcaaacc tgaacctgct
     5281 gcatcacgtg catgcggcac tcaaggcaca cgccctgttc aagcgggacg tggattacat
     5341 ggtggccaac ggtgaggtgg tcatcgtcga tgagcatact ggtcgggcca tgccgggcag
     5401 gcgctggtcc gagggcattc atcaggccat cgaggccaag gaacgggtgg cgattcagaa
     5461 cgagacccag accctggcct cgaccacgtt ccagaactac ttccggctct acgacaagct
     5521 tgccggcatg acgggcaccg ccgacaccga ggcgttcgag ttccggcaga tctacggcct
     5581 ggacgtcgtc gtgatcccca cccacatgcc catggttcgg gaagacaaca acgatctggt
     5641 ttacctcacc atcgaggaaa agtacgacgc gatcgtcgag gacatcaacg actgcatcga
     5701 gcgcggccag ccggtgctgg tggggaccgc ctccatcgaa tcttccgagc ggctgtctgg
     5761 ggagcttgat cgccggaaga tcgctcacaa cgtgctcaac gccaagcagc acgagcgcga
     5821 ggccgacatc attgcccagg ccggcagccc gggcgcggtg accatcgcca cgaacatggc
     5881 cggtcgaggc acggacatcg tgctcggcgg caactgggaa gccgaggcgg cccagctgga
     5941 ttcgcctacc gatgaggagc tgcagcgcct gcgcggcgaa tggcagaagc gccacgatgc
     6001 ggtcattgct gccggtggcc tgcacatcat cggcaccgag cgccacgagt cgcggcgcat
     6061 agacaaccag ctccgcggcc ggtcggggcg tcagggcgac ccgggctcgt cccgcttcta
     6121 tctttccatg gaagacaacc tgatgcggat attcgcatcg gaccgcatca ggggaatcat
     6181 gcagtccatc gggctcgaga agggagaggc catcgagcat cgcatggtga acaacgccat
     6241 cgagaaagcg cagcggaagg tcgagggcca caacttcgac atccgcaaga accttctgga
     6301 gttcgacgac gttgccaacg atcagcgcca ggtgatctat cagcagcgac gggagctgat
     6361 ggcggcggac gacatcgcgg ccgacatcga agggcttcgc gaagaggtcg ttctggacct
     6421 gatcgcagag tacatccctc cgcagagcat cgaggagcag tgggacgtcg atggcctgga
     6481 gcaggccctg cagaccgagt ttgccagcag gcagccggtg agaggttggc tcgacgagga
     6541 cgacgcgctc aacgaggatt cgttgtcgcg caagatcctg gctgaggtcg aggcggagta
     6601 tcgcgccaag gagcaggatt gggcgtccca cgggatcgac atgagggtgg tcgagaagca
     6661 gatcatgctg caggttctgg accagcgctg gaaggagcat ctggcggcca tggactacct
     6721 gcgccagagc attcatctgc gtgcctacgc ccagaagcag cccaagcagg aatacaagcg
     6781 ggagagcttt cagctgttcc aggaactgct ggagaacatc aagcgggacg tgatccgcct
     6841 gctctgccgg gtgcagatcg agacctccga cgagttggag gaagccgagc gccgtcggcg
     6901 cgaagaggcc gaacgtcgca tgcgttacac ccatgccgag tccagcgggc tcgacggggg
     6961 tgagcaggag cagccggacg atggcgccgg caaacccgag accttcgtga ggagtgagcg
     7021 gaaggtgggc cgcaacgagc cttgcccgtg cggctcgggc aagaagtaca aacagtgtca
     7081 cggcaaggct gcgtaggggg aagcccgaca tggcggtgaa cgttcctccg ctgcaggaac
     7141 tgctggtggt ccccggtgtg tctttgggta ctgcaggggc tgccatcaag agacagggcc
     7201 gtgacgacat cgccgtcctg ctgctgcacc cccagaccag ggcggcgggc gtgttcacgc
     7261 gcagctcgtt tcgagccgct cccgtgaccc tggccgatcg gcgactttcc ggcgggcgcg
     7321 cgcgagctct ggtgatcaac agcggtaacg ccaacgcggc caccggggat cccggtcttg
     7381 ccgatgccgt tgccgtctgc gatgcggttg ccgccgccct ggatgtgccc ggtgattccg
     7441 tgctgccgtt ctccaccggg gtcatcggtg agcgtctccc cgtttccaga atgctgccgg
     7501 cggtcgtttc cgcctgtggg agcctggcgc cggacggctg ggaggccgcc gctcgcgcca
     7561 tcatgaccac ggacacggcg cccaaggccg tcagccgtcg cgtcagggtc gcgggcgaag
     7621 aggtggtgat caccggcatg gcaaagggag cgggcatgat caagcccgac atggccacga
     7681 tgctgggctt cgtctgctgc gacgctgccg tcgccgacgg ctgcctgccc gcgatggtga
     7741 gggaagtggc cggccgctcg ttcaatcgaa tcaccgtgga tggcgacacg tccaccaacg
     7801 acgccttcgt ggtctgcgcg acgggatgcg cggagaacgc gcccgtgacc gacgtcggct
     7861 ccgaaggcta cgcggagctt cgcgatggcc tggccgcggt ggctaccgag ctggctcaga
     7921 gcatcgttcg cgatggcgag ggagccacgc gattcgtcac cgtgcgcgtc tccggcggcg
     7981 agagtgcggc cgaatgtctc aaggtggcct acacggtagc cgagtcccct ctggtcaaaa
     8041 cggcgatatt tgccggcgat ccgaactggg ggcggttctg catggccatc ggcagggcgg
     8101 gcattgccga tctcgacacc ggtcgcgtcg ccgttcacct ggacgacgtg cccatcgccc
     8161 tgggcgggct cgtggcgccg cagtacgagg aagccgccgc tgccagggtg atggcaaggc
     8221 aggagttcga agtccacgtc gacctcggcc gcggcagcgc cgaggagacg gtctggacaa
     8281 ccgatctgtc ctacgagtac gtgcgcatca acgctgagta tcgcacctga tccatggaag
     8341 cttctcggcc tggccggtca gtccgggtcc agatcccccg agagcagatc gtcgtccacc
     8401 tcgttgcccg gaattctgtg ctgctcgctg gcccaggcgc cgaggtccat gagcttgcac
     8461 cgctcggagc agaagggtcg gaactcgttg ccggacgtcc atttgacgcg gcccccgcag
     8521 gtcgggcagg tgacgatgcg cgcggtccgg tcgggttcgg aggcggtcat tgcgccatct
     8581 ccgtgtagac ctggtgcagg cgcgccacct cctcgtccag cacctccagg ctcgagtcgt
     8641 tgacgatgac gtcgtcggcg agggccaggc gctgctcgcg ggatgcctgg gcctcgatga
     8701 tctttctcac ctgggcctcg tcgttggcgt cccgggccat ggttcgccgc acctgtactt
     8761 cctccggcac gtccaccacc agaatgcgat gacagaatcg cgactggccg gtctcggcca
     8821 gcagcgggct gacgagcacg gcgtagggag actcggcctg ttcaagctcc tcgcggatgt
     8881 gctcgttgat cagcgggtgg gtgagctgct ccagccagcg gcgctcgtcg gggtctgcga
     8941 acaccctgcg tcgaagctcc gcccgatcga gtgatccgtc cgcctgtatg acatcgcagc
     9001 caaagtgctc ggcgatggcg acgagggctg gtcggcctgg ctcgactatc acgcgggagg
     9061 cgatgtcggc gtcgacgacg gttaccccca gcgctttgaa ccggtcggag acggcggtct
     9121 tgccgctgcc gatgccgccg gtgattccga tcaccagtct gctcattggg atgtcgtttg
     9181 aggtcggagg accggtcagg ctgcaaacag ggtcagcacg ttatcacggg ccataagcga
     9241 aacccagccc gcaaccgcca ggaagggccc aaatgggatc ggctgcgctg cctcccgttt
     9301 gccggccagc gtgatcgcca gggcatacgc gagccccagc gtcgcggcga tgaggatcgt
     9361 gcccggcagc acctgccatc cgagccacgc tccgatggcc gcgaagagct tgaagtcgcc
     9421 atagcccatg ccctccttgc cggtgatgag cttgaaagcc cagaaggtcg tccagagaaa
     9481 caggtagccg atcacggcgc cgaggacccc ggagcccaga tccgtgacgc cgccgaagtg
     9541 gcaggtgatc aggcccagcc agagcagcgg cagcgtgatc tggtcgggaa gcagctgggt
     9601 gtcgtagtcg atgaacgtca gcgcgatcat tgcccaggtg tacacagcgg cggctagacc
     9661 cagccacgtg tacccgaaga gcgccagcac cgttatggtc gcggccccgg tgagcacctc
     9721 caccatgggg tagcggatgg agattctgac gccgcagctt cggcagcggc ctttcaaaaa
     9781 gagccagctg aagaccggaa cgttctgcca cgccgagatg ggctgctgac aggcggggca
     9841 gcgcgaacgc ggcaccatga ggttgaaacg ctcctgcccg gtttccgctg gttcctcttc
     9901 gaggagttcg cgcgcctgcg cctcccattg ccgtgagagc atgatgggca gtcggtagat
     9961 gaccacgttg aggaagctgc ctaccgacag gcccacccac accgcaaagg tgaggccggc
    10021 ccatccgtag gcaagaagtg tttcccccat gacgtgagcg ctagcccggt cgcatacgat
    10081 tcgccgtgcc gagcctgggt taacccccga cgacccgacc cagctggaag atgggcaggt
    10141 acatcgcgat gatgaggccg ccgaccagca cgccgagaac ggccatgatc atcggctcca
    10201 tgagcgcggt gaggttgtcc acggcgttgt cgacctgctc ttcgtaatag gacgccgact
    10261 tatccagcat ctcgtccagc gcgccggcct cttcgccgat ggcgaccatc tgcaccacca
    10321 tgttgggaaa cacgccggtg gagcgcatgg agaagttcag ctgctgaccg gtgctcacgt
    10381 catcgcgtat ctggtaaacc gccttggtgt aaacggagtt gccggtggat ccggctacag
    10441 aattcagagc ctctaccaga ggtacgccgg cggcgaaggt ggtggccagc gtacgtgcgt
    10501 aacgggccac ggcggatttc tcgatgatgt tgccgaagat gggtgctttc aacgcaatcc
    10561 tgtctatcgc ctcgcggagt ttcttcgagc gttttcgcgc ggccatgaag gtggcgatgg
    10621 tgccgccgat agcaacgacg atcagaaacc accattgctg cgtgaactcc gagatgttta
    10681 tcacgaactg ggtgaaggcg ggcagctccg ctccgaaacc tgcgaagacc tcctcgaatt
    10741 gaggcaccac cttgatgagc aggatgcctg aaacgatgaa cgccaccacc aggacggcgg
    10801 ccgggtaggt catggccttg cggaccttgg ccttcaggga ctccgttttc tctttgtacg
    10861 tcgcgatgcg gtcgagcatg gtttccagag caccggattg ctcaccggct ccgaccaggt
    10921 tgcagaaaag gtcgtcgaag tgcagaggat gcttgcgcag cgctgcggtg aacgagctgc
    10981 cgcctgccac gtcgtcgcgc agtgcccgga tgagggtggc gaccttgggc ttgtcgaccc
    11041 cgcctgccac gatttcgaat gcctgtacga gcggcacgcc ggctctcatc atcgtggaca
    11101 tctgccgcgt gaacaaggcg atgtcgacag gcttgacgct gccgcctccg cccagcgaaa
    11161 aggccttgct cttcttcttt accttggtgg cgttgatgcc ctgacggcgc agttccgctt
    11221 tcgcgatggc cggtgtggag ctcgatatct cgcccttggt cttgcgaccg cctttgtcta
    11281 tgccttccca gaggaagatt gcgctttcgg ccatgtgtca gtgccccgtc gtcatcctgt
    11341 tcgcttcggc cagactggtc acgccttgta ttactttcag cagacctgat tggcggagat
    11401 ttttgaagcc gagtttgctt gcagtttccg ccagcttaat gctgtttccg tcctccatga
    11461 taatacggga catttctggc gtaattttaa ctacttcata aattccgact cggcccttgt
    11521 agccattgtt gcagctgtca cagcccacgg gctcgaagag ctcgaaaccc tcgtcgatct
    11581 gctcatctgt aaacccttct gccttgagcg tttcctt
//
LOCUS       DBNHKV010000278         3814 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1102661, whole genome shotgun sequence.
ACCESSION   DBNHKV010000278 DBNHKV010000000
VERSION     DBNHKV010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1102661"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..531)
                     /locus_tag="AC16PP_08795"
     CDS             complement(106..531)
                     /locus_tag="AC16PP_08795"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043023 - ribosomal large subunit binding
                     [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA];
                     GO_process: GO:0045947 - negative regulation of
                     translational initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HIR6481765.1"
                     /translation="MPRVERSIEIAAPVSRVQQIIVDFERYPEFVPFMERAELLGIEG
                     ERRRVRFTLNLVARISYTLDLVPGTDSGLTWTLVDGPFVRNNGSWRLKATNDDGTLAT
                     YTADVELKGFLPGFLERRLVAESLPATLRAFRDRAESDA"
     gene            complement(539..2557)
                     /locus_tag="AC16PP_08800"
     CDS             complement(539..2557)
                     /locus_tag="AC16PP_08800"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6481766.1"
                     /translation="MSGNDFVPKQAAAGLLLLAVVGCGSEEAPPADAGREQALPAGTN
                     SGHLVAYSEVREPCANADPLRIPLFGDVHVHTSFSFDAAANSVGTTPADAHKFALGEP
                     IPFFPVDASGTPTGTIRIDRPLDFLAVTDHGEFLGERALCRDPGSPKYSSAFCEEYRT
                     GERHGMRMLGRVITSETPRRVPELCGDDGALCLEYAEGPWRRIVEAAEAAYDRSPGCT
                     FTSFVGYEYTGTPGTSNYHRNVIFRNASVPPRPVSYIDAPVDSLLWNALDAACGAVDG
                     CDYLTIPHNSNLANGRMGPYRGIELTEANRRAYAEQRQRREPVMEMFQHKGASECING
                     LTTVLGEPDELCDVEAVRVIGRAETYQTARMVDGELVLGETTEVTQECAPGESSSNGM
                     LGAGCVSATDYMRSGLLVGLAEEQEIGVNPIKLGMIAATDTHTSTPGAVAEGDWRGHV
                     TGEATPAERLEPGLLTSGIDGNPGGLAGVWAVENSRDAIFDAMLRREVFGTSGPRIVP
                     RFFAGWGYDAEMCGSADLVARGYGGGVPMGGDLPRGAPGDRPAFIAWAARDPAPSAQP
                     LESLQLVKGWIDAEGQARNRVIPIAGGEGQVARDTLCAVYRDDDFDPAVPAYYYLRVV
                     EPPTPRWSTFDCQRLPESDRPAVCSDGSYPQTIREMAWTSPIWYRPEG"
     gene            2681..>3814
                     /locus_tag="AC16PP_08805"
     CDS             2681..>3814
                     /locus_tag="AC16PP_08805"
                     /inference="COORDINATES: protein motif:HMM:NF025114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6481767.1"
                     /translation="MPDNDDRISEPAADGELDVAIVGAGFSGLYMLHRVRDVLKLKTR
                     VFEAADGVGGTWYWNRYPGARCDSESYFYCYSFSKDLEQEWTWTSKFPEQPEILSYLN
                     HVADRFDLRRDIQLNTRVTGALFEDRSNRWIISTDTGETVSARFLISAVGCLSTRNLP
                     DIPGIESFAGEHYHTGNWPHEGVDFRGKRVGLIGTGSTGIQAAPVIAEEAEHLYVFQR
                     TPNFSVPARNTPLTAERTAEIKANYDEIRRISRTSGFGFPYETPEHAAMEVTPEERQS
                     VYEQRWHEGGTSFGFRSFSDILSDQAANDSASDFIRSKIRETVKDPEVAELLIPHDHP
                     YGTKRPPLDTNYFETYNRDNVTLVDVRSAPIKEITPRGVRTARD"
BASE COUNT          685 a         1304 c         1218 g          607 t
ORIGIN      
        1 gagctcatcg acgactacat cgaggggcgc ctgcccaagc gttgatccag gcggcctgaa
       61 gaagccgaac cctccggggg cggcagccgt ggggcgaccg gctgcctagg cgtctgactc
      121 ggctctgtcg cggaacgcgc gcagggtggc gggaaggctt tcggcgacga gccgtcgttc
      181 caagaatccc ggcagaaaac ccttgagctc gacgtcggcg gtgtaggtcg ccagggtccc
      241 gtcgtcgttc gtggccttaa gacgccagct gccgttgttg cgcacgaagg gcccgtctac
      301 cagggtccat gtcaggcccg aatcggtgcc gggcacgagg tccagggtat agctgatccg
      361 cgctaccaga ttcagcgtga agcgaacgcg gcgtcgttcg ccctcgatac ccagcagctc
      421 cgcgcgttcc atgaagggaa cgaactccgg atagcgttcg aagtcgacga tgatctgctg
      481 cacgcgcgat acaggcgccg cgatctcgat gctgcgctcg accctgggca tctgccgctc
      541 agccctcggg ccggtaccag atgggagacg tccaggccat ttcccggatc gtctggggat
      601 acgagccgtc gctgcacacg gccggccggt cggattcagg cagacgctga cagtcgaagg
      661 tgctccaacg cggcgtcggc ggctccacca cgcgcaggta gtaatacgcg ggcacagcgg
      721 gatcgaagtc gtcatcgcga taaacggcgc aaagggtgtc ccgcgctacc tgcccctctc
      781 cgccagcaat gggaatgacc cggttgcgcg cctgaccctc ggcgtcgatc catcctttga
      841 ccagctgcag cgactcgagc ggctgggccg agggcgccgg atcccgcgcc gcccaggcga
      901 tgaaagcggg ccggtcaccc ggggcacccc gggggagatc gccgcccatg ggcaccccgc
      961 ccccatagcc tcgagccacc aggtcggcgc tgccgcacat ctcggcgtcg tagccccagc
     1021 ccgcaaaaaa gcgcggcacg atgcggggcc cggaggtgcc gaacacctcc cgccggagca
     1081 tggcgtcgaa tatggcatcg cgcgaatttt ccacggccca gacgcctgcc aggccgccgg
     1141 gattgccgtc gatcccgctg gtcagcagcc ccggctcaag gcgctcggcc ggcgtcgctt
     1201 cgccggtcac gtggccgcgc cagtcgcctt ccgccacggc cccgggagtg gaggtgtggg
     1261 tgtccgtggc ggcgatcatg ccgagcttta tgggattgac cccgatttcc tgctcctcgg
     1321 ccaggcctac caggagcccg gagcgcatgt aatccgtggc agacacgcag ccggcgccca
     1381 gcatgccgtt gctggaagac tccccgggcg cgcattcctg ggtgacctcg gtggtctccc
     1441 ccagcaccag ctcaccgtcg accatccgcg ccgtctggta cgtctccgcg cggccgatca
     1501 cccggacggc ctccacatcg cacagctcgt ccggctcgcc gagcacggtg gtcagcccgt
     1561 tgatgcactc cgatgccccc ttgtgctgga acatctccat gacgggctcc cggcgctgac
     1621 gctgctccgc gtaagcacgc cgattcgcct cggtgagctc gatgccccga taagggccca
     1681 tccgtccgtt ggccaggttc gagttgtgcg gaatggtcag atagtcacaa ccatcgacgg
     1741 caccgcaggc cgcatccagc gcgttccaga gcagactgtc cacgggcgca tcgatgtaag
     1801 acaccggccg cggcgggacg ctggcattgc ggaagattac gttgcggtgg tagttcgagg
     1861 tgccaggcgt gcccgtgtac tcgtagccga cgaagctggt gaacgtgcag ccgggcgagc
     1921 gatcgtaagc ggcctcagcg gcctcgacga tccggcgcca cggaccctca gcgtattcca
     1981 gacacagcgc gccgtcgtct ccacacagct cgggcacccg tcgcggcgtc tccgaggtga
     2041 ttaccctgcc cagcatgcgc atgccgtgtc gctcgccagt ccggtactcc tcgcagaaag
     2101 cggacgagta cttcggcgag cccgggtccc ggcacagcgc ccgctcgccc aggaactccc
     2161 cgtggtcggt caccgcgagg aaatccagcg gccgatcgat gcggatggtg cccgtcggcg
     2221 tgccgctcgc gtctaccgga aagaagggta tgggctcgcc aagcgcgaac ttgtgcgcat
     2281 ccgcgggcgt ggtacctacg ctgttcgccg cggcgtcgaa cgagaagctg gtgtgaacgt
     2341 gaacgtcgcc gaacaggggt atgcgcagcg gatcggcgtt ggcgcaaggc tcgcgcactt
     2401 cgctgtaggc aacgagatgc cccgagttcg tgccggcagg caacgcctgt tcccgcccgg
     2461 cgtccgccgg gggcgcctct tcacttccgc agccgacgac ggcgagcagc agcaagccgg
     2521 ctgccgcctg cttgggaacg aaatcgtttc cgctcacgtc aaatccctcc cgatcctcgc
     2581 gaccgtcaac cttagcatgg agccgatcgg ccgcgccggt cacgacttgc aggtcacggc
     2641 gaaaccggca aactagaaag aaggtcggag ggagagaagc atgccagaca acgacgacag
     2701 gatttccgag ccagcggcgg acggcgagct cgacgttgcc atcgtcggcg cgggcttttc
     2761 cggcctctac atgctgcacc gggtgcgcga cgtgctgaag ctgaagacgc gcgtcttcga
     2821 ggcggcggac ggggtcggcg gcacgtggta ctggaaccgg tacccaggtg cccgctgcga
     2881 ttcggaaagc tacttctact gctactcgtt ctccaaggat ctcgagcagg agtggacgtg
     2941 gacgagcaag ttccccgagc aaccggaaat tctcagctac ctcaatcacg tggcagaccg
     3001 gttcgatctg cgccgtgaca tccagctcaa cacacgagtc accggcgccc ttttcgaaga
     3061 tcggagcaac cgctggataa tctccaccga caccggcgag accgtgtccg cccgctttct
     3121 gatcagcgct gtgggttgcc tctcgacccg gaacctgccg gatattcccg gcatcgagag
     3181 ctttgccggc gagcactacc acacgggcaa ctggcctcat gaaggcgtcg acttccgggg
     3241 aaagcgcgtg ggcctcatcg gcacgggctc caccggaatc caggcggcac cggtgatcgc
     3301 cgaggaagcc gagcacctct acgtctttca gcgcacgccg aatttcagcg tccccgcccg
     3361 caacaccccg ctgacggccg agcgaacggc agagatcaag gccaactacg acgagatccg
     3421 acggatctcg cgcacttcgg gtttcggctt tccttacgag acgccggaac acgcagccat
     3481 ggaggttaca cccgaggagc gtcagtccgt ctacgagcag cggtggcacg agggaggtac
     3541 cagcttcggc tttcggtcgt tcagcgacat tctgtctgac caggcggcca acgacagcgc
     3601 gtcggacttc atccggagca agatccgtga gaccgtgaag gaccctgagg tcgcggagct
     3661 cctcattccc cacgatcacc cctacggcac caaacgtcca ccactggata cgaactactt
     3721 cgagacctac aaccgcgaca acgtcaccct ggtcgacgtc cgatcggccc ccatcaagga
     3781 gatcactccc cgcggggtca ggaccgcacg ggat
//
LOCUS       DBNHKV010000279         7988 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1951409, whole genome shotgun sequence.
ACCESSION   DBNHKV010000279 DBNHKV010000000
VERSION     DBNHKV010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7988)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7988)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7988
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1951409"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="AC16PP_08810"
     CDS             <1..703
                     /locus_tag="AC16PP_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR6481768.1"
                     /translation="MMGGIPTGVLGQALTQDAQGDDREIPGLYAAGEAACVSVHGANR
                     LGGNSLLDLIVFGRAAGLHIEEVMRQGVSYRDPSDSDLEQAQARLNRWDESGPGETVA
                     DLKKELQTTMQNSFGVFRTGTHMEEGIKKIAELRDRISRAHLGDKSKVFNTARLEALE
                     LDNLLEVAEATAIAANARTESRGAHAREDFQVRDDENWLCHSLFFSDERRVTKRAVNF
                     SPETVDTFQPMERKY"
     gene            712..1419
                     /locus_tag="AC16PP_08815"
     CDS             712..1419
                     /locus_tag="AC16PP_08815"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HIR6481769.1"
                     /translation="MMQVSVYRYNPETDEVPAMREMSIELPGDRDLMVLDVLEMLKAE
                     DPTLSYRRSCREGVCGSDGINMNGRNGLACITPVSSVAKRGKLVLRPLPGLPVIRDLV
                     VDMTQFYRQYEKIKPYLINDKPPPAQERLQSPEERAELDGLYECILCACCSTACPSFW
                     WNPDKFIGPAGLLQAYRFLADSRDTATAERLSELDDPFSVFRCRGIMNCVSVCPKGLN
                     PTRAIGKIRTMLLQQGT"
     gene            1508..4345
                     /locus_tag="AC16PP_08820"
     CDS             1508..4345
                     /locus_tag="AC16PP_08820"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018925108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HIR6481770.1"
                     /translation="MADSFRERMERSSHLAGANAAYIEAMYERYLDEPNEVPPEWREY
                     FDTLPRVEGAVGADVPHSTIVDQFERLGRNRLKARPERESHFAQNVHEQKQMRVQDLI
                     SAYRHRGHKKANIDPLGMMERPAMPILDLAYHHLSLADLDETFNVGSFHYGSPEAKLR
                     DLVDALERTYCTSIGFEYMHIVDQSEQLWVQQRLESVRARPVLTEDHKRVILERLTAA
                     EGLEKYLHGRYPGTKRFGLEGGESLIPMLHELLQRLGSHGVLEAVIAMAHRGRLNVLV
                     NILGKDPRLLFDEFDGKLPVNEDDMGDVKYHQGFSSNVMTPGGEMHLALAFNPSHLEI
                     VGPVVEGSVRARQDRRRDHVGTRVVPILVHGDASFAGQGVVMETLQMSQTRGFKTGGT
                     IHIIINNQIGFTTHRQDDARSTEYCSEVAKLVQAPIFHVNGDDPEAVLFASQLAADYR
                     MEFKKDVVIDLVCYRRRGHNEAEEPMKTQPLMYKKIRQHPTTRAIYVERLIGEGLVED
                     SEADGLAEAYRNTLDQGGHVALSLVKEPNTELFVDWTPYLGHDTVMPADTRVDLSVIQ
                     ALARRLEELPDGFVLQRQVQKIMEDRHKMSFGAMPINWGFAETMAYATLLDEGYGVRL
                     TGQDVGVGTFSHRHAALHNQKDGKRYIPLNSLSETVNFDLYDSLLSEEAVLAFEYGYA
                     ATAPDKLVIWEAQFGDFANGAQVVIDQFISSGEVKWARLCGLTMLLPHGYEGAGPEHS
                     SARLERFLQLCAQHNMQVCLPTTPAQVFHMLRRQVVWPARKPLVALTPKSLLRHKLAV
                     STLEDLANGTFHTVLGEVDEIDPDGVERVVLCSGKVFYELLEARREANASQVAIIRLE
                     QLYPFPEAELERIMAPYGSVRDVVWCQEEPMNQGAWYASQHHMRRVIMKHNENIYLAY
                     AGRPAFAAPASGHAHLHGVRQQRLVEEALFG"
     gene            4366..5568
                     /gene="odhB"
                     /locus_tag="AC16PP_08825"
     CDS             4366..5568
                     /gene="odhB"
                     /locus_tag="AC16PP_08825"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HIR6481771.1"
                     /translation="MTIQIKAPTFPESIADGTVAAWHRNVGDQVQRDDLLVEIETDKV
                     VLEVVAPAAGRLTSVLKAEGETVLSEELIAELDETAAGEPKQEVEADEPQQAQQPEPA
                     DDEVSASPAARKLATESGLDIQRIEGTGKAGRITREDVAQAASARPEPPAPTPAAVDE
                     ASVDEGERVVRRVPMTRLRASIARRLVEAQQNAAMLTTFNEVDMGPIMTMRRRYQDEF
                     QARHNGTRLGFMSFFVRASVEALKRFPAVNASIDENDILYHGYYDIGVAVSTERGLVV
                     PVIREADALSLAQIEDQVNDFAARAQDGKLSIEDMSGGTFTISNGGVFGSLLSTPILN
                     PPQTAILGMHKIQDRPVAVDGEVVVRPMMYLALSYDHRLIDGQEAVRFLVTIKGLIED
                     PARILLEL"
     gene            5571..7010
                     /gene="lpdA"
                     /locus_tag="AC16PP_08830"
     CDS             5571..7010
                     /gene="lpdA"
                     /locus_tag="AC16PP_08830"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR6481772.1"
                     /translation="MNDKYDVIVIGAGPAGYHAAIRAAQLGLSTACIDKSLDADSNPA
                     YGGTCLNWGCIPSKALLDASHKFVEAKEHFAEIGIRTGELSVDVGQMMARKRQVVQGL
                     TGGVAALFKSNGVTDLPGRGRLLASRQVEYTPHDGDAVVLQAEHVILAPGSVPMEIPP
                     APLTKDLIVDSTGALEFEEVPERLGVIGAGVIGLELGSVWSRLGSEVVVLEALDEFLP
                     MTDERIARDALKIFRNQGLDIRLGTRVMGAEVTDGEVTVTYQDQDGDQSLKVDKLIVA
                     VGRRPFTEGLLAPDCGVNLDERGFLYVNDLCATDAPHVYAVGDVVRGPMLAHKGMEEG
                     VMVAERIAGFKPLVNYECVPAVIYTHPEVAWVGKTEQELKASGDEYNVGSFPFTASGR
                     AMAANDVQGQVKVLADAETDRILGVHVLGPQASELIAQAVIAMEFDACAEDLGLTMFA
                     HPTLSEAVHEAALSVAGHAIHTVNRKKQK"
     gene            7023..>7988
                     /gene="sucC"
                     /locus_tag="AC16PP_08835"
     CDS             7023..>7988
                     /gene="sucC"
                     /locus_tag="AC16PP_08835"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIR6481773.1"
                     /translation="MNLHEYQAKGLFADYGLPVSQGYAVDTADEAVAAAERIGGGRWV
                     VKVQVHAGGRGKAGGVKLADSLDEVRAFADQWIGKNLVTFQTDEHGQPVSKIYVESCT
                     DIDRELYLGAVVDRGTRRVVFMASTEGGVEIEKVAEETPEKILRVAIDPFVGPQPYQG
                     RQLAFRLGLEGAQIREFTNLFMGLSRLFQELDCSLLEINPLVITTAGNIHCLDAKVNI
                     DGNAMFRQPALAAMRDVSQEDEREAEAAEYDLNYVALDGNIGCMVNGAGLAMGTMDLV
                     KIHGGNPANFLDVGGGATKEAVSEAFKIILSDDNVKAVLINIFGGI"
BASE COUNT         1553 a         2433 c         2610 g         1392 t
ORIGIN      
        1 catgatgggc ggtattccca ccggcgtgct cggccaggcg cttacccagg acgcccaagg
       61 cgacgaccgc gaaatccccg gtctttatgc tgctggagag gccgcatgcg tctccgtgca
      121 tggcgccaac cgactcggcg gcaactcgct gctcgacctc atcgtgttcg gtcgggccgc
      181 ggggctgcac atagaggaag tgatgcgtca gggcgtttcc tatcgggatc ccagcgacag
      241 tgatctcgag caggctcagg cccgtctgaa ccgctgggac gaaagtggtc cgggcgaaac
      301 ggtcgccgac ctcaagaagg agctgcagac gacgatgcag aacagcttcg gcgtgttccg
      361 gaccgggact cacatggaag agggcatcaa gaagattgcg gagctgcgcg atcgcatctc
      421 tcgagcccat ctcggcgaca agagcaaggt cttcaacacg gcgcggctag aagcccttga
      481 gctggacaac ctgctcgagg tggcagaggc caccgccatt gccgccaacg cccgcacgga
      541 gagccgaggc gctcacgccc gggaggactt ccaggtgcgc gacgacgaga actggctttg
      601 ccactcgctg tttttctcgg acgaaagacg ggttaccaag cgagccgtga acttcagccc
      661 tgagaccgtg gacaccttcc agcccatgga acggaagtac tagagaggat ggtgatgcag
      721 gttagcgttt atcgatacaa cccggagacc gacgaggtcc cggccatgcg cgagatgagc
      781 atcgagctcc ccggcgaccg ggatctgatg gttctcgacg ttctcgagat gctgaaggcc
      841 gaggatccta ccctgtccta ccggcgctcc tgccgggagg gtgtttgcgg ttcggacggc
      901 atcaacatga acgggcgaaa cgggctcgcc tgcatcacgc cggtttcctc tgttgcaaag
      961 cggggcaagc tcgttctgcg gccgcttccc gggctgccgg tgatccggga cctggtggtg
     1021 gacatgactc agttctatcg ccagtacgaa aagatcaagc cgtacctcat taacgacaag
     1081 ccgccccctg cccaggagcg cctgcagagt ccggaggagc gtgccgagct cgacgggttg
     1141 tacgagtgca tcctgtgtgc ctgctgctcc accgcctgcc cctcgttctg gtggaaccct
     1201 gacaagttca tcggtccggc gggtctgttg caggcctatc ggttcctggc cgacagccgg
     1261 gacacggcga cagccgagcg gctctcggag ctggacgacc ccttcagcgt cttccgatgc
     1321 cgcgggatca tgaactgcgt gagcgtttgc cccaaggggc tcaaccccac gcgggccatc
     1381 ggcaagatcc gcaccatgct tctgcagcag gggacgtaga cgaacccctt gcgcggctat
     1441 actgctgctt caacggacaa aaggctgacg gctctcacgg tgagctccta tcccacgcag
     1501 gctgacaatg gctgactcgt ttcgcgagcg catggagcgt agttcgcacc tggccggtgc
     1561 caacgctgcc tacatcgagg ccatgtacga gcgctatctc gacgaaccga acgaggtgcc
     1621 ccctgagtgg cgcgagtatt tcgacacgct gccgcgcgtg gaaggggctg tcggtgccga
     1681 tgtgccccac agcaccatcg tcgatcagtt cgagcggctg ggacgcaacc gcctgaaagc
     1741 gagaccggag cgcgagagcc actttgcgca gaacgtgcac gagcagaagc agatgcgcgt
     1801 tcaggatctc atctcggcct atcggcaccg gggccacaag aaggcgaaca tcgacccgct
     1861 gggcatgatg gaacgacccg ccatgcccat cctcgatctg gcctatcacc atctctccct
     1921 cgcggatctg gacgagacgt tcaacgttgg atcttttcac tacggatcac cagaggcgaa
     1981 gctgcgcgat ctcgtggatg ccctcgagcg gacctactgc acgtccatcg gtttcgagta
     2041 catgcacatc gtcgatcaga gcgagcagct ctgggttcag cagcgcctcg aaagcgtgcg
     2101 tgcgcggccg gtactgaccg aggatcacaa gcgggtgatc ctggaacgcc tgaccgctgc
     2161 agaaggcctg gaaaagtacc tgcacggccg ttatcccggt accaagcgtt tcggcctgga
     2221 aggcggggag agcctcatcc ccatgcttca cgagctgctg cagcggctgg gttcccatgg
     2281 cgtcctcgag gccgtcatcg ccatggcgca tcggggccgg ctcaacgtgc tggtcaacat
     2341 cctgggaaaa gacccccggc tccttttcga tgagttcgac ggcaagctgc cggtgaacga
     2401 agacgacatg ggagacgtca agtaccacca gggattttcc agcaacgtga tgacgccggg
     2461 cggcgagatg cacctggcgc tggcgttcaa tccttcccac ctggagatcg tcgggcccgt
     2521 ggtggaggga tccgtgcggg cgaggcagga tcgccgccgc gaccatgtgg gaacccgggt
     2581 cgtgcccatc ctcgttcacg gcgacgcgtc cttcgccggg cagggagtgg tcatggagac
     2641 cctgcagatg tctcagaccc gagggttcaa gacgggcggg acgatccata tcatcatcaa
     2701 caaccagatc ggcttcacca cccatcgtca ggacgacgcg cgctccaccg agtactgctc
     2761 ggaggtggcc aagctggttc aggcccccat cttccacgtc aacggcgacg atccggaggc
     2821 ggtgctgttt gcttcgcaac ttgccgccga ttaccggatg gagttcaaaa aggacgtggt
     2881 gatcgatctc gtctgctatc gccgcagggg tcacaacgag gccgaagagc ccatgaagac
     2941 ccagccgctg atgtacaaga agatccgtca gcacccgacc acccgggcga tatacgtgga
     3001 acgcctgata ggggaagggc tcgtggagga cagcgaggcc gatggccttg ccgaggcgta
     3061 ccggaacacc ctggatcagg gagggcacgt ggccctgagc ctggtgaagg aaccgaacac
     3121 cgagctgttc gtggactgga cgccctatct gggccacgac accgtcatgc ccgcggatac
     3181 ccgcgtcgat ctcagcgtga tacaggcgct cgccaggcgg ctcgaggagc tccccgatgg
     3241 tttcgtgctt cagcgccagg tccagaagat catggaagat cgacacaaga tgagcttcgg
     3301 ggcgatgccc atcaactggg gatttgccga gaccatggcg tacgcaacgc tgctggacga
     3361 gggttacgga gtccgcctga cgggccagga cgtgggcgtc gggacctttt ctcaccggca
     3421 tgccgctctc cacaaccaga aggacggcaa gcgctacatc cccctgaaca gcctctctga
     3481 gaccgtcaac ttcgacctct acgacagcct gctttcggaa gaggcggtgc tcgccttcga
     3541 gtacggttac gccgcgaccg cgccggacaa gcttgtcatc tgggaggccc agttcggtga
     3601 ctttgccaat ggtgcccagg tggtcatcga ccagttcatc tccagcggcg aggtgaaatg
     3661 ggcccggctg tgcggtttga ccatgctgct gccccacggc tatgagggcg ccggacccga
     3721 gcactcgtcg gcgaggctcg agcggtttct ccagctctgc gcccaacaca acatgcaggt
     3781 ctgccttccc accacgccgg ctcaggtgtt tcacatgttg cgtcgtcagg tggtctggcc
     3841 cgcgcgcaag ccgctggtgg cgctcacgcc aaagagcctg ctcaggcaca agctcgccgt
     3901 gtcgacgctc gaggacctgg cgaacgggac gtttcacacg gtgctgggcg aagtcgacga
     3961 gatagatccg gacggcgtcg agcgcgttgt gctctgcagc gggaaggttt tctacgagct
     4021 gctggaagcc cgtcgggagg cgaacgcgag tcaggtcgcc atcatcaggc tcgagcagct
     4081 ctacccgttt cccgaggcgg agctcgagcg catcatggcg ccgtacggct cggtacggga
     4141 cgtggtctgg tgtcaggagg aacccatgaa ccagggtgcc tggtacgcct cccagcacca
     4201 tatgcgccgg gtcatcatga agcacaatga aaacatctac ctggcctacg ccgggcgccc
     4261 ggctttcgcc gccccggcca gcgggcacgc gcatctgcac ggcgtgcggc agcagcgcct
     4321 ggtggaggag gccctgttcg gctaacgggc tatgaaggac cgccgatgac catccaaatc
     4381 aaagcgccaa cgtttccgga atccatcgcc gacgggaccg tagccgcctg gcaccggaac
     4441 gtgggcgatc aggtccagcg cgacgatctg ctggtggaaa tagagacgga caaggtggtg
     4501 ctcgaggtgg tggcacccgc ggcaggacgc cttaccagcg tcctgaaagc cgagggggaa
     4561 acggtcctca gcgaagagct cattgcggag ctcgacgaaa ccgcggcggg cgagcccaag
     4621 caagaagtag aggctgacga gccgcagcag gctcagcagc ccgagcccgc ggacgacgag
     4681 gtcagcgcga gcccggcagc caggaagctg gccacggaga gcggcctgga catccagcga
     4741 atcgaaggca cgggcaaggc tggccggatc acgcgcgaag acgtggccca ggccgcctcg
     4801 gcccggcccg agccgccagc accgacgccg gcagcagtcg acgaggcttc cgtcgacgag
     4861 ggtgaacgtg tggtgcgccg tgtgccgatg acgcgcctgc gcgcgagcat cgcccgccgg
     4921 ctggtcgagg cccagcagaa tgctgccatg ctgaccacgt tcaacgaggt cgacatgggg
     4981 cccatcatga ccatgcggcg tcgctatcag gacgaatttc aggcgcgtca caacgggacc
     5041 cgcctcgggt tcatgtcttt cttcgtgcgc gccagcgttg aagccctcaa gcgcttcccc
     5101 gccgtgaacg cttccatcga tgaaaacgat atcctgtatc acggctacta cgacatcggc
     5161 gtagcggtga gcaccgaacg cgggctcgtc gtgccggtaa tccgtgaagc cgacgcgctc
     5221 agccttgcgc agatcgaaga tcaggtgaat gatttcgccg cgcgcgcgca ggacgggaag
     5281 ctttccatag aggacatgtc ggggggtacg ttcaccatct ccaatggcgg ggtttttggg
     5341 tcgctgctgt cgacgccgat tctcaacccg ccgcagacgg ccattctcgg catgcacaaa
     5401 attcaggatc gcccggtggc ggtggacggt gaggtcgtgg tcagaccgat gatgtacctc
     5461 gcgctgtctt acgatcaccg gctcatcgac ggtcaagagg ccgtgcgatt cctggttacc
     5521 atcaagggcc tgattgaaga ccccgctaga attctgctgg aactttaaca atgaatgaca
     5581 agtacgacgt gatcgtcatc ggcgctggcc ctgcgggcta ccatgccgcc atccgcgcag
     5641 cgcagctggg gctcagcacc gcctgcatcg acaaatccct ggatgccgac agcaaccccg
     5701 cgtacggggg cacctgcctg aactggggtt gtatcccgtc caaggcattg ctcgatgcct
     5761 cccacaagtt cgtcgaggcc aaggagcatt tcgctgagat cggcattcga accggcgagt
     5821 tgagcgtgga tgtcggtcag atgatggccc gcaagcggca ggtggtacag gggctcaccg
     5881 gtggcgttgc ggcgctgttc aaaagcaacg gcgtcaccga cctgcctgga agggggcgcc
     5941 tcctggccag ccggcaggtg gagtacacgc cccatgatgg cgacgccgtg gtgctgcagg
     6001 ccgagcacgt gatccttgcg ccgggttccg tgcccatgga gatcccgccg gcgccgctga
     6061 ccaaggatct gatcgtcgac tcgaccggtg ctctcgagtt cgaagaggtg cctgagcgac
     6121 tgggcgtgat cggcgccggg gtgatcggtc tggagctcgg cagcgtctgg agtcgcctcg
     6181 gctccgaggt ggtggttctg gaagcgctgg atgagtttct tcccatgacc gacgagcgaa
     6241 ttgcccggga tgcgctgaag atctttcgca atcaggggct tgatatccgc ctggggaccc
     6301 gggtaatggg ggcggaggtc accgatggtg aggtaaccgt gacgtatcag gatcaggatg
     6361 gggatcagtc gctcaaggtg gacaagctca tcgtggcggt gggcaggcgg cccttcaccg
     6421 aagggctcct cgcgcccgat tgcggggtca atctggacga gcgcggcttc ctttacgtca
     6481 acgatctctg cgctaccgat gccccccacg tctacgccgt gggcgacgtg gttcgtggcc
     6541 cgatgctcgc gcacaagggc atggaggagg gcgtcatggt cgccgagcgc atcgccggct
     6601 tcaaacccct cgtcaactac gagtgcgtac cggcggtgat ctacacacac cccgaggttg
     6661 cctgggtggg caagaccgag caggagctca aagccagcgg ggatgagtac aacgtgggca
     6721 gtttcccgtt cacggccagt ggccgggcaa tggcagccaa cgacgtgcag ggccaggtca
     6781 aggtgcttgc tgacgccgag actgaccgga ttctgggcgt gcacgtgctg gggcctcagg
     6841 cttcggagct cattgcccag gcagtcatcg ccatggagtt cgacgcgtgc gcggaggatc
     6901 tggggcttac gatgttcgct catccgacat tgagcgaagc cgtccacgaa gccgcgctga
     6961 gcgtcgccgg acatgccata catacggtca atcgtaagaa acagaaatag aacggaagtc
     7021 ttatgaatct acatgagtat caggccaagg ggctgtttgc cgactatggg ctgccggtgt
     7081 cgcaggggta tgcggtagac acggcggacg aagccgtggc cgccgccgag cggatcggcg
     7141 gggggcgttg ggtggtcaag gtgcaggtgc acgcaggcgg tcgcggcaag gcgggtggcg
     7201 tcaagctggc agacagcctc gacgaggtcc gcgccttcgc cgatcagtgg atcggcaaga
     7261 acctggtgac tttccagacc gatgagcacg gccaacccgt cagcaagatc tacgtggaaa
     7321 gctgcacgga catcgaccgg gaactctacc tgggcgccgt cgtcgaccgg ggcacccggc
     7381 gggtggtttt catggcgtcc accgagggtg gcgtcgagat cgagaaggtg gcagaggaga
     7441 cgccggagaa gatccttcgc gttgccatcg atccctttgt agggccacaa ccctatcagg
     7501 gccgtcagct ggccttccgg cttggtctag agggcgctca gataagggaa tttacaaatc
     7561 ttttcatggg cttatccagg ctatttcagg aactggattg ctcgcttctg gagatcaacc
     7621 cgctggtcat taccacggcg gggaatattc attgccttga tgcgaaggtc aacatcgacg
     7681 gcaacgcgat gtttcgccag ccagcccttg cggccatgcg cgacgtctcc caggaggatg
     7741 aacgggaagc cgaagctgcc gagtacgatc tgaactacgt cgcgctcgac ggcaacatcg
     7801 gttgcatggt gaacggcgcg ggcctggcca tgggcaccat ggatctggtg aagattcatg
     7861 gcggcaaccc ggccaacttc ctcgacgttg gcggcggcgc gaccaaggag gcagtttcgg
     7921 aggccttcaa gatcattctc tcggatgaca acgtcaaggc ggtgctgatc aacatcttcg
     7981 gcggcatc
//
LOCUS       DBNHKV010000280         5199 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1102914, whole genome shotgun sequence.
ACCESSION   DBNHKV010000280 DBNHKV010000000
VERSION     DBNHKV010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1102914"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="AC16PP_08840"
     CDS             complement(<1..434)
                     /locus_tag="AC16PP_08840"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR6481774.1"
                     /translation="MTPTRALAERIAGTQYADIPAPARTVAKQALLDFIGVTMAGIDE
                     PLARMLRDQVNEEGGHPQAQLIGTDERASTVQAALVNGSAGHAHDYDDVHTAMTGHPT
                     VPVAPAVLALAERLGRSGEEAIAAFCAGVDTECILGHYAGPG"
     gene            600..1448
                     /locus_tag="AC16PP_08845"
     CDS             600..1448
                     /locus_tag="AC16PP_08845"
                     /inference="COORDINATES: protein motif:HMM:NF018677.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HIR6481775.1"
                     /translation="MPERVYCSEFNGDEPMLGTADVVDVWLMLEYRPAWKARALEESA
                     LAAPTRRWLAAGIDALAAAGLRARPQLVRQPELDRDTVSLLVAFGDRLLEFQGTGYDF
                     LNGVDLAGLARGHLPEGARVVSEPRYFVCTNGQRDLCCARFGLPVYAAMRERLGSRVW
                     QVSHLGGHRFAPNVLVLPQATLYGRVQPADVPAFIERVESGEVSLAHLRGRTEYAKPV
                     QAAAGYCGGGELDLVDARGDDAAATVTFRRGSEEITVAVERSDPVDVLKSCGKASEPA
                     YPYRRR"
     gene            complement(1396..2256)
                     /locus_tag="AC16PP_08850"
     CDS             complement(1396..2256)
                     /locus_tag="AC16PP_08850"
                     /inference="COORDINATES: protein motif:HMM:NF039989.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR6481776.1"
                     /translation="MPHIVDTLIEERARGLMQHPLLWRLVTGLLYPVLGYRAAVRMAD
                     TVADMGGLEIFEMLSAQLKLDIDLGGTEHIPETGSAVVTANHPTGIADGVAVFDVLRT
                     VRSDITFFANRDAIRFAPGLADVIVPVEWVEDKRTHARNRETVSHMVRAFRSERLIVI
                     FPSGRLARPTLRGLVERGWTPTAVNLAQKYRCPIVPMHIRGRNSWLYYLLYAINTELK
                     DMTLFRELLNKTRQPYRIRIAEPFDPVGDARMLTIALREFIADEMPGGERRFSVSDTD
                     TLAPTPFRSS"
     gene            complement(2244..3020)
                     /locus_tag="AC16PP_08855"
     CDS             complement(2244..3020)
                     /locus_tag="AC16PP_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6481777.1"
                     /translation="MHLDWQIHDGKGPHLLLVHGFLCSRAQWLWNLTVLAEVCRPVTV
                     ELWGHGRSPAPSDPACYAPDRIVEEFESIRTALGAEQWFVLGYSLGAGLTIRYALTHP
                     HRVMGHAFTNSTSGLADAEQIRRWQDEADETAQRIIAAGQAALERIPVHPRHATKLPD
                     PLHQALMADAARLDPTGVANVLRHTNPNVSVRADVHRNTRPALLVCGSRERRFQPHRA
                     FAQATMPCLEIVDIPAGHGVNMEAHEPFNAAVSSFVRCHT"
     gene            3136..4827
                     /locus_tag="AC16PP_08860"
     CDS             3136..4827
                     /locus_tag="AC16PP_08860"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6481778.1"
                     /translation="MAYDILIRNGTVVDGSGGAPYPADVGIRNGRIASIGRIREKART
                     TIDAEGHVVSPGFVDGHTHMDAQVFWDPIGSSSCYHGVTSVVMGNCGFTLAPCRRDEA
                     DLVFRNLERAEDLSRDAMLTGIDWQWETFPQFLDAVDGLPKGINYAGYIGHSALRTYV
                     MGERAFTDPATDGDLAAMCRQVQEAVRAGAIGFSTSRTFNHITADDRPVASRLAEWDE
                     VRAIVNAMGETGKGIFEIAGEAPGRDPARIRDYHERLRDLAVESGVTQTWGMFSVRIA
                     PDFWRPYFDLLDETAAAGGRMYAQVHSRALNVLLSFKTSTPFDKWDVWNDVRSLPLDE
                     QKAKLRDPALRARLVEAANREYQGPRVVGAEARPPNWEWVYPMADMSYDQPSMAELAA
                     ERGVDPVELMIDMSLAHDFDIFFRQPIANENQDDVLEMMKHPRAMVTFSDSGAHVSQI
                     MDSSLQTHLLSYWVREKQAFTLPEAVRRITYDTATMWGLHDRGLVRAGMAADLVIFDP
                     ETIGPRMPTVEHDLPAGARRLKQTADGIMHTIVNGEVLLTRNEYGGATPGRLLRA"
     gene            complement(4875..4951)
                     /locus_tag="AC16PP_08865"
     tRNA            complement(4875..4951)
                     /locus_tag="AC16PP_08865"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4915..4917),aa:Arg,seq:tcg)
     gene            5076..>5199
                     /locus_tag="AC16PP_08870"
     CDS             5076..>5199
                     /locus_tag="AC16PP_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR6481779.1"
                     /translation="MNELPLVGVTVLDFGQVYNGPYCGFLLAQAGARVIKVESPR"
BASE COUNT          835 a         1656 c         1841 g          867 t
ORIGIN      
        1 tgaccgggcc cggcgtagtg gccgaggatg cactcggtat ccacgcccgc gcagaacgcg
       61 gcgatggcct cttctccgga ccgacccagc cgctcggcga gagcgagcac ggcgggcgcg
      121 acgggcacgg tgggatgtcc ggtcatggcc gtatgcacgt cgtcatagtc gtgagcgtgg
      181 ccggctgagc cgttgaccag ggcggcctgg acggtggacg cccgctcgtc ggtgccgatg
      241 agctgtgcct gggggtgccc gccttcctcg ttgacctgat cgcgcagcat gcgggcgagg
      301 ggctcgtcga tgccggccat ggtcacgccg atgaagtcga gcagcgcctg cttcgccacc
      361 gtgcgtgccg gcgccgggat gtcggcgtat tgagtgccgg cgatccgctc ggccaacgcg
      421 cgggtcggtg tcatccgtgg tcctcccgaa agcctccgga cggagactgt tgagccctaa
      481 gctagcggga ttgccatggc tttgcaaagc cgcctgaggc cctgttttag cgccgccggg
      541 tacaggctcg gaacgatttc tgcttcaatg gccgtctccc ggcgaggcga ggcgacccca
      601 tgcccgagcg cgtctattgc agcgagttca atggcgacga gcccatgctc ggcacggcgg
      661 acgtggtgga cgtctggctg atgctcgaat accggccggc ctggaaagcc agggccctgg
      721 aggagagcgc gctcgcggcg ccgacgagac gctggctggc cgccggcatc gatgccctgg
      781 cggccgcggg gctgcgggct cgcccccagc tcgtccgcca accagagctc gacagagaca
      841 cggtttcgct gctggtcgcc ttcggcgacc ggttgctcga attccagggg acaggctacg
      901 actttctgaa cggcgtggat ctcgccgggc tggcgcgggg ccatctgccc gaaggcgctc
      961 gcgtggtctc ggaaccgcgc tacttcgtct gcaccaacgg ccagcgggat ctgtgctgcg
     1021 cgcgcttcgg ccttcccgtt tacgccgcga tgcgggagcg gctgggcagc cgggtgtggc
     1081 aggtgagcca tctcggcggt caccgcttcg cgccgaacgt gctcgttctg ccccaggcga
     1141 ccctgtacgg ccgcgttcag cctgctgacg tgcccgcctt catcgagcgc gtcgagtccg
     1201 gcgaggtgtc gcttgcgcac ctgcgggggc gcacggagta cgcgaagccc gtgcaggcgg
     1261 ccgcggggta ttgcgggggc ggggagctgg atctggtcga cgcccggggc gacgatgccg
     1321 ccgccacggt gacgttccgg cgcgggtccg aggagatcac cgtggcggtg gaaagatccg
     1381 accccgtgga cgtgctcaag agctgcggaa aggcgtcgga gccagcgtat ccgtatcgga
     1441 gacgctgaat cgccgttcgc cgccgggcat ctcgtcggcg atgaactcac gcagcgcgat
     1501 ggtcaacatc ctcgcatcgc ccacggggtc gaacggctcg gcgatgcgga tccggtacgg
     1561 ctggcgggtt ttgttgagca gctcgcgaaa cagcgtcatg tccttcagct cggtgttgat
     1621 ggcgtacagc agatagtaga gccaggagtt cctgccgcgg atgtgcatgg ggacgatggg
     1681 gcatcggtac ttctgcgcca ggttcaccgc cgtaggcgtc cacccgcgct ccaccaggcc
     1741 tcggagagtg ggccgggcca ggcgcccgga aggaaatatg acgatcagcc tctcggaccg
     1801 aaacgcgcgg accatgtggc tgacggtctc ccggttccgt gcgtgcgttc gcttgtcttc
     1861 cacccactcg acgggcacga tcacgtcggc gaggcccggc gcgaagcgaa tggcatcccg
     1921 attggcgaag aacgtgatgt ccgacctcac cgtgcgcagc acgtcgaaca cggcgacgcc
     1981 gtcggcgatg ccggtggggt gatttgctgt gaccaccgcg ctgcccgttt cggggatgtg
     2041 ctcggtgccg ccaaggtcga tgtcgagctt cagctgcgcg ctgagcatct cgaatatctc
     2101 gagcccgccc atgtcggcca ccgtatcggc catgcggacc gccgcgcgat accccagcac
     2161 gggatacagc aggccggtca ccagccgcca cagcaggggg tgctgcatca gtccccgggc
     2221 ccgttcctcg atgagcgtgt cgactatgtg tggcatcgga cgaacgagct gaccgccgcg
     2281 ttgaacggtt cgtgggcttc catgttgacg ccgtggccgg ctggaatgtc gacgatctcg
     2341 aggcagggca tggtcgcctg ggcgaacgcg cgatggggct gaaagcgccg ttctctcgag
     2401 ccgcagacga gcagagcggg acgggtgttg cgatgcacgt ccgcgcgcac ggacacgttc
     2461 gggttggtgt ggcgtagaac gttcgcgacg cccgtcgggt cgagacgtgc ggcgtccgcc
     2521 atgagggcct ggtgcagagg atcagggagc ttggtcgcgt gacggggatg tacggggatg
     2581 cgttccaggg cggcctgacc tgcggcgatg atccgctgcg cggtctcgtc cgcctcgtcc
     2641 tgccagcgcc gtatctgctc ggcgtccgcc aggccggagg tcgagttggt gaaggcgtgt
     2701 cccatgaccc gatggggatg ggtcagcgcg tagcggatgg tcaggccggc accgagggag
     2761 tagccgagca cgaaccattg ctcggcgccg agggccgtgc ggatggactc gaactcttcc
     2821 acgatccgat ccggcgcgta gcacgccgga tcggacggtg ccggcgatcg accgtggccc
     2881 cagagctcca cggtgacggg gcggcagacc tcggccagga cggtcaggtt ccacagccat
     2941 tgggcgcggc tgcacagaaa gccgtggacc agcagcaggt gcggtccctt gccatcgtgt
     3001 atctgccagt cgagatgcat gtaaacggct gcccttggga tgacgcaaaa taagacatta
     3061 accgatggcc cgccatacgg acggtgtcgc tccgtcgcgg acgccggtga tagactgcca
     3121 cgcaaggagg gcggcatggc ttacgacatt ctgatcagaa acggaacggt ggtggatggt
     3181 tccggtgggg cgccttaccc ggccgacgtg ggcattcgca acgggcgtat cgcgtccatc
     3241 ggcaggatcc gggaaaaggc ccggactacc atcgatgccg aggggcacgt ggtcagcccg
     3301 ggattcgtgg acggccatac gcacatggac gcccaggtgt tctgggatcc catcgggtcc
     3361 agctcctgct atcacggcgt gacgagcgtg gtcatgggca actgcggctt caccctggca
     3421 ccgtgccggc gggacgaggc ggacctggtc ttccgcaatc tcgaacgtgc cgaagatctc
     3481 tcccgggacg ccatgctcac ggggatcgac tggcagtggg agacctttcc gcagtttctc
     3541 gatgcggtgg acggcctgcc caaggggatc aactacgccg gctacatcgg tcattcggcc
     3601 cttcggacct acgtcatggg cgagcgcgct ttcaccgacc cggccaccga cggggatctg
     3661 gcggccatgt gccggcaggt gcaggaagcc gtgcgcgcgg gcgccatcgg cttctcgacg
     3721 tcgcgcacgt tcaaccacat caccgccgac gaccgtcccg tggccagccg tctggcagag
     3781 tgggacgagg tgcgcgccat cgtcaacgcc atgggcgaga cgggcaaagg catcttcgag
     3841 atcgccggcg aagcgccggg ccgtgatccg gctcgaattc gcgactacca cgaacggctt
     3901 cgcgacctgg ccgtggagtc cggggtgacc cagacctggg gcatgttcag cgtgcgcatc
     3961 gcgcccgatt tctggcgacc ctatttcgat ctcttggacg agaccgcggc agcgggaggg
     4021 cgaatgtacg cccaggtgca cagccgggcg ctcaacgtgc tgctgtcgtt caagacctcg
     4081 acgccgttcg acaagtggga cgtctggaat gacgtccgct cgctccccct ggacgagcag
     4141 aaggcgaagc ttcgcgatcc cgccctgcgc gcccggctgg tcgaggccgc caatcgcgag
     4201 taccagggcc ctcgtgtggt gggcgccgaa gccaggccgc cgaactggga gtgggtctac
     4261 cccatggcgg acatgagcta cgatcagccc agcatggccg agctggccgc cgagcgcggc
     4321 gtggatcccg tggagctcat gatcgacatg tcgctggcgc acgacttcga catcttcttc
     4381 cgtcagccca tcgccaacga aaatcaggac gacgtgctgg aaatgatgaa gcatccgcgg
     4441 gcgatggtga ccttctccga ttccggcgcc cacgtctctc agatcatgga cagctccctg
     4501 cagacccacc tcttgagcta ctgggtgcgg gagaagcagg cgttcaccct gcccgaggcg
     4561 gtccggagga tcacctacga tacggccacc atgtggggcc tgcacgatcg cggtctggtt
     4621 cgggcaggca tggcggcgga cctggtgatt ttcgaccccg aaaccatcgg cccgcggatg
     4681 cccaccgtgg agcacgacct gccggcgggc gctcgccggc tcaagcagac ggccgacggg
     4741 atcatgcaca ccatcgtcaa cggcgaggtg ctgctgaccc gcaacgagta cggcggagcg
     4801 accccgggca ggctgctgcg cgcctgagct cgctcgagca ggcggccgct cggggctgcc
     4861 aggaagaagg gatctggcgc ggctgggagg actcgaaccc ccgacacctg ggttcgaagc
     4921 ccagtgctct atccagctga gctacagccg ctctggaaga gtcggtcagt ataaccgtat
     4981 ccacctgggc gggcgctgcc ttttcccgtg agcagtggtg gctggccagc aaaccggcta
     5041 ccgttcgtgc agagacgatg ctggtggggt gcgcgatgaa cgagctgccg ctggtcggtg
     5101 tgaccgtgct ggatttcggc caggtctaca acggaccgta ctgcgggttc ctgcttgccc
     5161 aggcgggcgc gcgggtcatc aaggtcgagt cgccgcggg
//
LOCUS       DBNHKV010000281         3490 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1741789, whole genome shotgun sequence.
ACCESSION   DBNHKV010000281 DBNHKV010000000
VERSION     DBNHKV010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1741789"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..709
                     /locus_tag="AC16PP_08875"
     CDS             230..709
                     /locus_tag="AC16PP_08875"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR6481780.1"
                     /translation="MMRKFISLAFAALCIPVLATADVYRWVGPDGVVNYTQMKPEGVQ
                     AERVQAQGGRRSTAAPAPSEPADQSATQSADTSQLSPKQQEMLNDLQSAEAARKQEVA
                     RIRESNCERSRRVLDRLSATARIRVRDDSGEERIMGEDERQNRIAEAQQGVAENCLS"
     gene            complement(748..1500)
                     /gene="pgeF"
                     /locus_tag="AC16PP_08880"
     CDS             complement(748..1500)
                     /gene="pgeF"
                     /locus_tag="AC16PP_08880"
                     /inference="COORDINATES: protein motif:HMM:NF014619.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HIR6481781.1"
                     /translation="MVEANAWEWARWPDDAPGHRIAAVTTTRPGGFSKGKYAAFNLAC
                     HVHDDPAHVARNRRDLLKALGLAHVQWLEQVHGTGCVQATLASCDRVPVADAAWTSVP
                     GLALAVLTADCLPVVVAARDASTVAVIHAGWRGLVAGIIPSVVGSLPGRTTGYVGWIG
                     PGIGADAYEVGGDVAEAVRQSGPEAEAYLRPGGRADKYQLDLAGLAVRQLGDVGIGCV
                     ERSSACTWSDARFYSYRRDGETGRQATLVWIR"
     gene            complement(1493..2461)
                     /gene="rluD"
                     /locus_tag="AC16PP_08885"
     CDS             complement(1493..2461)
                     /gene="rluD"
                     /locus_tag="AC16PP_08885"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HIR6481782.1"
                     /translation="MAEPLEIQKRVTVPPDVAGERVDKAAARLFPEFSRAALTAWIQD
                     GALTVDGARVKPKVKLVGGETLVLDAVVAPREDWSTGEPVPFDVLYEDADLLVVNKPA
                     GLVVHPGAGNPDGTLVNGLLHHRASLALVPRAGIVHRLDKDTSGLLLVAANLEAHAAL
                     VRALQARQIQRGYRAVCEGVLVSGLDIDAPMGRDPVRRTLQRVRADGRPALTRVRVVE
                     RFRAHTLVVAQLETGRTHQIRVHLSSIGHPLVGDRAYGARGRLPPGAQDELIAALRGF
                     SRQALHAAKLAFDHPVHGGRLQFEAPDPDDLVDLLDALRQDVRHHG"
     gene            2549..>3490
                     /locus_tag="AC16PP_08890"
     CDS             2549..>3490
                     /locus_tag="AC16PP_08890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR6481783.1"
                     /translation="MRKFTLMPFLLPLLLLCLASSGCSWMPWVGKDKDKAEEDLNATE
                     QQLYRDTQRALRAGNYDRAIQGLQQLEARFPFGRYAEQAQLEIIYAQYMSYDHDTARA
                     SADRFMRLHPQHANIDYAYYIKALAAFNKNRGLLDRIFATDLAKRDMTAARDAYADFA
                     QLLALYPDSQYAPDANLRMIYLRNLLAESEIHVADYYMRRGAYLAASNRARYVIETYS
                     KSQSVDDALAILVEANWRLGLEEAANDALRVLALNYPNYPAFDTDGNLTLASTVRNRD
                     RSWTNMVTFGLLDRPKVPPPITIKHPEGFEPPPVNPDA"
BASE COUNT          653 a         1225 c         1046 g          566 t
ORIGIN      
        1 cggggtttga aaggagggac cgccggagcc gcgtattctt ggggttcgcc cacggcatgt
       61 caacgaacgg tccaatgcgc caaattgcga atcgcttcac atatccgggg taactgccgc
      121 aagggacgga cgccccggtc gctctaaaat cccggattgg attgctacgc ccgggcgccg
      181 gtcacgaaaa aaaccgccgg cggccgtggc atatatcagg agtatctgga tgatgcgcaa
      241 gttcattagt ctcgccttcg ctgctctctg cataccggta ctggcgaccg ccgacgtcta
      301 ccgctgggtc ggtcccgacg gggtcgtcaa ctacactcag atgaaacctg agggtgtcca
      361 ggccgagcgc gtccaggccc agggcggaag acgttcgact gctgccccag ccccaagcga
      421 gcccgccgac cagtccgcca cccagagcgc ggacacgtca cagctctccc ctaagcagca
      481 ggaaatgctg aacgatctgc agtcggctga ggccgcccgc aagcaggaag tcgcccggat
      541 ccgggaatcc aactgcgagc ggtcacgacg ggtgctcgac cgcctgtccg ccaccgcccg
      601 catacgcgtt cgtgacgatt ccggcgagga aagaatcatg ggcgaggatg agcgccagaa
      661 tcgcatcgct gaagcccagc agggcgttgc agagaactgc ttgtcctgag gacccggggc
      721 ccgcagccgt gcgggcccac tgccagctca ccgtatccag accagcgttg cctggcgacc
      781 ggtctcgcca tcgcgccgat acgaataaaa cctggcgtcg gaccaggtgc atgccgagga
      841 tcgctcgaca catccgatcc ccacgtcccc aagctgccga acggccagcc cggccaggtc
      901 aagctgatat ttgtccgccc gtccgcccgg tctcaggtag gcttccgcct cggggcccga
      961 ttgccgcacc gcctcggcca cgtctccgcc aacctcataa gcgtcggctc cgattcccgg
     1021 accaatccag cccacgtatc ccgtcgtcct gccaggtagc gagcccacca cgctggggat
     1081 gatgccggca accaggccgc gccaacccgc gtggatcacg gcaaccgtgc tcgcatcgcg
     1141 agcagcaacg accaccggca ggcaatcggc ggtaaggacg gccagcgcaa gcccgggaac
     1201 agacgtccac gcggcatcgg ccaccggcac ccggtcgcag cttgccaggg tggcctggac
     1261 acagcctgtt ccatggacct gctcgagcca ttgcacgtgc gcaagaccga gcgctttgag
     1321 cagatcgcga cggtttcggg ccacatgggc cgggtcatcg tggacgtggc aggcgagatt
     1381 gaaggctgca tacttcccct tgctgaaccc gcccggccgg gtcgtggtta ccgcggcgat
     1441 tcggtggccg ggcgcgtcgt ccggccacct ggcccactcc caagcgttcg cttcaaccat
     1501 ggtgccgcac gtcctgccgc agggcatcga gcaaatccac cagatcgtcg gggtccggag
     1561 cttcgaactg caacctcccg ccgtgaacgg ggtggtcgaa ggcgagcttc gcggcatgca
     1621 gcgcctggcg cgagaaaccg cggagcgcgg cgatcagttc gtcctgagca cccggtggca
     1681 gacgtccccg agccccgtag gctcggtccc ccaccagcgg atgcccgata ctgctcagat
     1741 gcactctgat ctggtgggtg cgtccggttt ccagctgcgc gaccaccagg gtgtgagccc
     1801 tgaaacgctc gaccacccgg acccgggtga gcgcggggcg accatcggcc cgaacgcgct
     1861 ggagcgtcct gcgcacggga tcgcgcccca tcggggcatc gatatccagc ccgctgacca
     1921 gcaccccttc acagacggcg cgatagcccc gctgaatctg tctggcctgc agcgccctga
     1981 caagagcggc gtgagcttcg agattggccg caaccaggag cagcccggac gtgtccttgt
     2041 ccagacggtg cacgatcccc gcccgcggaa ccagggccag cgaggcccgg tgatggagca
     2101 gcccgttgac gagcgtgcca tcgggattgc ccgctccagg atgaaccact agccccgcag
     2161 gcttgttgac gacgagcagg tcggcgtctt catagaggac gtcaaacggg acgggctccc
     2221 cagtgctcca gtcttcccgg ggcgcgacga cggcatcgag cacgagtgtt tcgcccccta
     2281 cgagcttcac ttttggtttg acccgggcac cgtccaccgt gagcgcccca tcctgaatcc
     2341 aggcggtcaa cgcggcgcgg gagaattccg ggaacagccg ggccgccgcc ttgtcgacgc
     2401 gctctcccgc cacgtcaggt ggcaccgtta ctcgtttctg gatttccagc ggctccgcca
     2461 tcgccctctg ctctggttgc aaccgtacgc taaatcgtta aattagcgaa actttctggc
     2521 tcacgcctga accaaagctg caggatccat gcgcaaattc accctcatgc cctttctttt
     2581 gcctctccta ctgctttgcc ttgcgtcttc cggctgctcc tggatgccct gggtcggcaa
     2641 ggacaaggac aaagccgaag aggacctgaa cgcgacggag cagcagctct accgcgacac
     2701 gcaacgcgcg ctccgggccg gcaactacga ccgggcgatc cagggtctcc agcagctcga
     2761 ggcccggttt cctttcggcc gctacgcgga gcaggcccag ctggagatca tctacgctca
     2821 gtacatgtcc tacgatcacg acacggcccg cgcgtccgcg gaccggttca tgcgcctcca
     2881 tcctcagcat gccaacatcg actacgccta ctacatcaag gctctggccg ccttcaacaa
     2941 gaaccggggc ctgctggatc gcatcttcgc gaccgatctt gcaaaacggg acatgaccgc
     3001 cgcccgcgac gcttacgccg acttcgctca gctgctggcc ctttaccccg acagccagta
     3061 cgcgcccgac gcgaacctgc ggatgatcta tctgcggaac ctcctggcag agtcggagat
     3121 tcacgtggcc gactactaca tgcgcagggg cgcttatctg gcggccagca accgggcgag
     3181 atacgtcatc gagacctact ccaagtccca atccgtagac gatgcgctgg ccatactggt
     3241 tgaggccaac tggcgcctgg ggctggagga ggccgccaac gacgcactgc gggtattggc
     3301 gctcaactac ccgaactacc cggcgttcga caccgacggc aacctgaccc tggcgagcac
     3361 cgtgcgcaac cgcgaccgat cctggaccaa catggtcacg ttcggtctgc tcgaccgtcc
     3421 caaggtcccg ccgccgatca cgatcaagca tcctgaaggc tttgaaccgc cacccgtcaa
     3481 tccggacgcc
//
LOCUS       DBNHKV010000282         3620 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_181797, whole genome shotgun sequence.
ACCESSION   DBNHKV010000282 DBNHKV010000000
VERSION     DBNHKV010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_181797"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1577)
                     /locus_tag="AC16PP_08895"
     CDS             complement(60..1577)
                     /locus_tag="AC16PP_08895"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008030174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR6481784.1"
                     /translation="MITRIAATTAALLLSFAAWGAEQPNILVIWGDDIGITNISAYSD
                     GIMGYRTPNIDRIASEGVRFTDYYGDQSCTAGRSTFITGQSGLRTGLTKVGLPGAELG
                     IQDRDVTLAEVLRDMGYATGQFGKNHLGDKDEHLPTNHGFDEFLGNLYHLNAEEEPED
                     PDYPKVPGFRERFGPRGVIHSFADGKVEDTGPLTRKRMETADEEFVAAAKDFVDRSVK
                     ADKPFFVWLNTTGMHFRTHPAEKHLGKSGQGPYNDVMMAHDELVGQMLNQLDALGIAE
                     NTIVFYSTDNGVHFNTWPDAGVTPFRSEKNTNWEGAYRVPAMVRWPGKIAPGQVSNEI
                     MSHLDWMPTLVAAAGDVGLKDKLLEGTRIGRKQSKLHLDGHNFLPYLTGQAENGPRRE
                     FHYLNDEGMPVGVRVGDWKIVFAENRGKTMALWAEPFVTLRMFKIYNLRRDPFERAEH
                     NSNSYWDWMIDKAPQGYLGLAATAQFLQSFQAYPPSQRPDSWSIDRLTEALLEQQ"
     gene            complement(1712..2743)
                     /locus_tag="AC16PP_08900"
     CDS             complement(1712..2743)
                     /locus_tag="AC16PP_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6481785.1"
                     /translation="MSETSRHCSKPQVPIIRAANLMGFIDFLEKTAGPADGCLGRAGL
                     SRELTKRPECLISFPRAIRFLELASAQIGTPHMGWYVGRDHPISALGSYGQRLDACVT
                     VHDYLRTGVSLYPRVSNRDSLKLEEVREGLRIHHDSGLPPSLGTHQSDLHFIEITLGK
                     LREALGPSWTPGRVGIAHSPSEPVPADSLLASAGVLTKSGTAFIEVSKAELRAVFPRR
                     RRPTNSCPPSVSLSSLPADFVGLARAQIEMLLPTGRIGIELLAESMGLSVRSVQRRIA
                     SAESSFSELLEDARFRLATSWLEHTSKSVTEIAFDLGYQYPANFTRAFRARTGLPPNA
                     YRATFSGSA"
     gene            3104..>3620
                     /locus_tag="AC16PP_08905"
     CDS             3104..>3620
                     /locus_tag="AC16PP_08905"
                     /inference="COORDINATES: protein motif:HMM:NF019157.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HIR6481786.1"
                     /translation="MAPHCKVVGVIGTETNFELLLPENWNGKFVMGGGGGFVGSVQNT
                     SLLLGSLQKGYATVGTDAGHAGHPLNASWAYNNLERLVSFGHQAVHRTAVTAKALITD
                     FYDEGISRSYFTGCSRGGGQGFMEAQRYPEDFDGIVAGSPAYDFLGVAAAATQISAAM
                     YPDPSNLQEAVV"
BASE COUNT          608 a         1086 c         1200 g          726 t
ORIGIN      
        1 ggccgcgccg ctgatccagc agatgcacag gaggcccccg gcgatggcgc tgcggacgtt
       61 cattgctgct cgagaagcgc ttcggtcagc cggtcgatgg accagctgtc cggccgctgg
      121 ctcggcgggt acgcctgaaa ggactgcagg aactgcgccg tggcggcgag gcccaggtag
      181 ccctgaggcg ccttgtcgat catccagtcc cagtaggagt tcgagttgtg ctcggcacgc
      241 tcgaagggat cacggcgcag gttgtagatc ttgaacattc tgagcgtgac gaacggctct
      301 gcccacagcg ccatcgtctt gccgcggttc tcggcgaaga cgatcttcca gtcgccgacg
      361 cgcacgccga cgggcatgcc ctcgtcgttt aagtagtgga actcccggcg cggcccgttt
      421 tcggcctggc cggtcagata gggcagaaag ttatggccgt ccagatgcag cttcgactgc
      481 ttacggccga tccgcgtgcc ctcgagaagc ttgtccttga gcccgacatc cccggctgct
      541 gccacgagcg tcggcatcca atccaggtgg gacatgatct cgttggagac ctgaccggga
      601 gcgatcttgc cgggccagcg caccatggcc ggcacgcggt aggcgccttc ccagttggtg
      661 ttcttttcgc ttcggaacgg ggtgaccccg gcgtccggcc aggtgttgaa atgcacgccg
      721 ttgtccgtgg agtagaagac gatggtgttc tcggcgatgc ccagcgcgtc cagctggttc
      781 agcatctgcc cgacaagctc gtcgtgggcc atcatgacgt cgttgtacgg gccctggccc
      841 gacttgccca ggtgcttctc cgccgggtgg gtgcggaagt gcatgcccgt ggtgttgagc
      901 cagacgaaga acggcttgtc ggccttgacg gaccggtcga cgaaatcctt cgcggcggca
      961 acgaattcct cgtccgccgt ttccatgcgc ttgcgcgtga gcggcccggt atcctctacc
     1021 ttcccatcgg cgaacgagtg gatcacgccg cgcggcccga accgttcccg gaagccgggg
     1081 acctttggat agtcgggatc ctcaggctcc tcctcggcgt tcaggtgata gaggttgccc
     1141 aggaactcgt caaagccgtg attggtgggc agatgctcat ctttgtcgcc caggtggttc
     1201 ttgccgaact gcccggtggc gtagcccatg tccctgagca cctcggccag cgtcacgtct
     1261 cggtcctgaa tgcccagctc ggcgccgggc aggcccacct tggtcagccc cgttcgcagg
     1321 cccgactggc cagtgatgaa agtggaccgg cctgccgtgc agctctgatc gccgtagtag
     1381 tcggtgaagc gcacgccctc gctggcgata cggtcgatgt tcggcgtgcg gtaccccatg
     1441 atgccgtcgc tgtacgcgct gatgttggtg atgccgatgt cgtcgcccca gatcacgagg
     1501 atgttcggct gctcggcgcc ccaggcggcg aagctcagca gcaatgcggc ggtggtcgcc
     1561 gctatccgtg tgatcatcat gttttcctct gcgcgtgccg cttcgagcgt gatgcggaaa
     1621 tcgctttgcc cgcaacgaag tttgcgatgc tacctccatt cgagggggcg atgaagcgcg
     1681 ggggtcaggt cgggcgatgc cgcttcgaaa gtcaggcgct tccggagaag gttgctcgat
     1741 aggcattcgg cggcaacccc gttctcgctc ggaacgctcg agtgaagttg gcagggtatt
     1801 gatatcccag gtcgaaggcg atctcggtga ccgatttgga agtgtgctcc agccagctag
     1861 ttgcgagtcg aaatcgagcg tcctccagca gctcggagaa actcgattcc gcgcttgcga
     1921 tgcgacgctg cacgctgcga accgacaacc ccatgctctc cgccagcagc tcgatgccga
     1981 tgcgtccggt cggcagcagc atttcgatct gcgctcgggc cagcccgacg aagtccgcgg
     2041 gaaggctcga gaggctgacg ctgggcgggc agctgttggt tggtctgcgt ctgcgcggga
     2101 aaacggcgcg tagctcggcc ttgctgactt cgatgaatgc cgttccgctt ttggtaagga
     2161 caccggcgct tgcgagcagc gaatccgcgg gcacgggttc gctggggctg tgcgcgatgc
     2221 ccacacggcc tggggtccag gaaggtccga gggcttcccg gagcttgccg agagtaatct
     2281 cgatgaagtg aagatcgctc tgatgggttc ctaaacttgg cgggaggccg gagtcgtgat
     2341 gtatccgcag gccctcgcgg acctcttcga gcttgaggga gtcccggttg cttactctcg
     2401 ggtaaagtga caccccggtg cgcaggtagt cgtgcacggt cacgcaggcg tcgagccgct
     2461 ggccatagct gcctagcgcg ctgatgggat gatcccgccc gacgtaccaa cccatgtgcg
     2521 gggtaccgat ttgggccgat gccagctcca ggaatctgat cgcccttggg aaagaaatca
     2581 gacattcggg acgcttcgtg agctcccggg aaagacccgc acgtcccagg cagccgtccg
     2641 ctggcccggc ggttttctcc agaaagtcga tgaagcccat caggttggcc gcacggataa
     2701 ttggcacctg tggcttgctg cagtgcctgc tggtctcgct catggttccc tcttgctcag
     2761 cgcctttgcg agggtggcgt caaatgataa gcgaatggcg gagcaggtga atatgcttgt
     2821 gaacatcgtc ggatcggcag tgccggtcgg aaactgcctc gggggttgag gtttctgttg
     2881 ccttacctga ggatctggct gattgcgatc gagagtggaa ggggacgaaa tgatgtttga
     2941 aaggccatta gatgtaggct tgttaattct gctgacagcc gtgctcacgg cgtgcggaac
     3001 ctcggagcag agcctgccgg cgacctcggg tccggtctgt tctcaggcat cgtttccggc
     3061 acttcccgac gtccggctca tctcggtcac gcaggaggcc tcgatggcgc cgcattgcaa
     3121 ggtggtcggc gtgatcggta ccgagaccaa tttcgagctt ctgctgccgg agaactggaa
     3181 cggcaagttc gtcatgggcg gcggcggtgg gttcgtcgga agcgtgcaaa acacctcgct
     3241 tttgctcgga tccctgcaaa aaggctatgc aacggtcggc accgacgccg gccatgcggg
     3301 gcatccgctc aatgcgagct gggcctacaa caacctggag cgcctcgtca gctttggcca
     3361 ccaggccgtc caccgcaccg cggtaacggc gaaggcgctg atcaccgact tttacgacga
     3421 gggcatcagc cgcagctact tcaccggatg ctctcgcgga ggggggcagg gcttcatgga
     3481 ggctcagcgg tatccggagg atttcgacgg catcgtggcc ggctcaccgg catacgattt
     3541 tctgggggtt gctgcggcgg ccacccagat cagcgcggca atgtaccccg atccttccaa
     3601 cctgcaggaa gccgtggtgg
//
LOCUS       DBNHKV010000283         3795 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_324856, whole genome shotgun sequence.
ACCESSION   DBNHKV010000283 DBNHKV010000000
VERSION     DBNHKV010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_324856"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="AC16PP_08910"
     CDS             <1..972
                     /locus_tag="AC16PP_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6481787.1"
                     /translation="IGLLADGQDNFAWALFGEVAADLTDQLELAVSLRYDKDERENTT
                     LTEPLYDPTLILGLVTGDKRKESWDDLQPKVTLRYKPQDNVTLYGGYSRGFRSGGFNQ
                     TGVGEAVPEPGVEDLFDEQTADTYEIGLKAILMDGRLSTSMAAYYTNFEGAYFFFYDA
                     LTSTQNLGSIDEVTYAGVEVEANAMLTDNFTAYLGFGYVDSEIDEAANTDHEGNQAPL
                     VSEYTLNVGLQYRQPLGAMNGRLELVGRVDYQRIGDTYWEPANITKRSPVDLVDLRLG
                     VEVPGDWALTAWAKNAFDKKYNAEYSPGGFLFKAPPARWGVDFTKRF"
     gene            995..1537
                     /locus_tag="AC16PP_08915"
     CDS             995..1537
                     /locus_tag="AC16PP_08915"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6481788.1"
                     /translation="MSNAPPVNSRLHHNAYVTENMEAVRRFYEEVIGFPLIATWCEQA
                     ELFGRERTYMHCFFDVGGGESLAFFQFADPSDAAEFGPELPSSPFRHIAMKVDQETQD
                     AIRDRLAAAGYKEPDTYVLNHGYCYSLYVVDPSKLIIEFTVDHEDVDQINADQRAKAH
                     AELARWLGGDHSPNNEAYHR"
     gene            1565..2599
                     /locus_tag="AC16PP_08920"
     CDS             1565..2599
                     /locus_tag="AC16PP_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen
                     decarboxylase/cobalamine-independent methonine synthase
                     family protein"
                     /protein_id="HIR6481789.1"
                     /translation="MAALECGITGSLPKPKGLSDTSAQLRADWMVADDALGEAHDDAV
                     RLAIADQVSAGLDIVTDGEQRRRHYIWGFLEALEGVDAHKLGKKQTRGARYTTELQNV
                     ARVVGDVRRSGPIFVDALKFAKAHSDRPVKVTLPGPMTIVDSVVDEHYGDALPDLAAR
                     FADILNAEARALADAGADIVQFDEPAFNVDTDAVAEWGIATLERVARGVATQRAVHIC
                     YGYGVPQVIAWKNQNQDWGHYGHTLPLLNESSIDQVSVECAASGVDVSVIQHLSNKRV
                     MLGVISVSTDEVETPELVAERIRAGLEFCDAERLMPCTDCGLVPRPRAVAQAKMAALA
                     AGTALVRDAL"
     gene            2643..3524
                     /locus_tag="AC16PP_08925"
     CDS             2643..3524
                     /locus_tag="AC16PP_08925"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6481790.1"
                     /translation="MCDEQTVRDNEAYLHTSAGMSRRQFAGAAAGAAIAMLLPRSGNA
                     LTVSGRDVRITTPDGEADAYFVHPAEGKHPGVIVWPDIRGLRPAFRQMADRLAGSGYA
                     VLVVNPFYRDARAPLVPEGASFQDEATRAVLMPLYNSITSTTTDVDAKALVDFLDAQT
                     EVDTTGGIGTMGYCMGGPMTLHSAAAVPDRVAAAASFHGSRLVTDAEDSPHLRVPGMS
                     ASFLIAIAENDDARDPQAKDVLELVFDDAGLAAEIEVYEGALHGWCPPDSSVYHEGQA
                     ERAWERLLVLFESALKS"
     gene            3596..>3795
                     /locus_tag="AC16PP_08930"
     CDS             3596..>3795
                     /locus_tag="AC16PP_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6481791.1"
                     /translation="MKYLARAILRLGGWRIEGAKPDLEKYVLVAAPHTSNWDFLWMIC
                     MAQALEIPVSWLGKDALFHPLIG"
BASE COUNT          677 a         1204 c         1266 g          648 t
ORIGIN      
        1 atcggtctgc tggcagacgg gcaggacaac ttcgcctggg cgctcttcgg cgaggtagcc
       61 gcggatctca ccgaccagct ggagctggcc gtctccctgc gttacgacaa ggacgagcgg
      121 gagaacacca cgctcaccga gccgctctac gatccgacgt tgattctcgg tctggttacg
      181 ggcgacaagc gcaaggagag ctgggacgat ctgcagccca aggtcaccct tcgctacaag
      241 ccccaggaca acgtaacgct ctatggcggc tacagccgtg gtttccgcag cggcggcttc
      301 aaccagacgg gcgtggggga agcagtgccg gaacccgggg tcgaggatct cttcgacgag
      361 cagacggcgg acacctacga gatcggtctg aaggcaatcc tgatggacgg ccggctcagc
      421 acctcgatgg ccgcctacta caccaacttc gaaggcgcgt atttcttttt ctacgacgcg
      481 ctcacctcca ctcagaacct gggcagcatc gacgaggtga cctacgccgg ggtcgaggtc
      541 gaggccaacg ccatgctcac cgacaacttc accgcctacc tgggcttcgg gtacgtggac
      601 agcgagatcg acgaggcggc aaataccgac cacgagggca accaggcacc gctggtatcc
      661 gagtacacgc tcaacgtcgg cctgcagtac cgccagcccc tgggcgccat gaacggtcgg
      721 ctggagctgg tgggccgggt ggactaccag cgcattggcg atacttactg ggagccggcc
      781 aacatcacca agcgcagccc cgtggatctg gtggacctgc ggctcggcgt cgaggtgccc
      841 ggagactggg cgctcacggc ctgggccaag aacgcgttcg ataagaagta caacgcggag
      901 tactctccgg gcggtttcct gttcaaagcc ccgccggcgc gctggggcgt ggatttcacc
      961 aagcggttct gagctgggca atagcgagga catcatgagc aacgcaccac cggtcaattc
     1021 acgtctgcac cacaacgcct acgtcaccga gaacatggaa gcggtgcgca ggttctacga
     1081 ggaggtgatc ggctttccgc tgatcgccac ctggtgtgag caggccgagc tcttcggcag
     1141 ggagcgcacc tacatgcact gcttcttcga cgtgggcggc ggtgagagtc tcgcgttctt
     1201 ccagttcgcg gatccttcgg acgccgccga gttcgggcct gagctgcctt cctcgccgtt
     1261 ccggcacatc gccatgaagg tggatcagga aacccaggac gccattcgcg accgcctggc
     1321 ggcggccggc tacaaggagc cggacaccta cgtcctcaat cacggctact gctactcgct
     1381 ctacgtcgtc gaccccagca agctcatcat cgagttcacg gtggatcacg aggacgtgga
     1441 tcagatcaac gcggaccagc gcgccaaggc tcatgcggag ctcgcccgtt ggctcggcgg
     1501 cgatcactcg ccgaacaacg aggcctacca ccgctaggat cacgaagttc ccggagaaag
     1561 gcccatggcg gctctagaat gtggcattac cggcagcctg ccgaaaccga aggggctgag
     1621 cgacacttcg gcgcagctgc gcgcggactg gatggtcgcg gacgacgctc tcggcgaggc
     1681 acacgacgat gctgtccggc tcgccatcgc cgatcaggta agcgcgggcc tggatatcgt
     1741 cacggacggc gagcagcgcc ggcgccacta catctggggg ttcctggagg cgctggaggg
     1801 cgtggacgcg cataagctgg gcaagaagca gacgcgcggg gcgcgttaca ccaccgagct
     1861 gcagaacgtc gcgcgcgtcg ttggggacgt gcggcggtcc ggccccattt tcgtggacgc
     1921 tctgaagttc gccaaggccc actccgaccg gccggtaaag gtcaccctgc ccggacccat
     1981 gaccatcgtg gattcggtgg tggacgagca ctatggcgat gcgctgcccg atctcgctgc
     2041 gcggtttgct gacatcctca acgcggaggc cagagcgctc gccgacgccg gagcagacat
     2101 cgtccagttc gacgagcccg ccttcaacgt ggacaccgat gcggtggccg agtggggcat
     2161 cgccacgctg gagcgcgtgg cgcggggcgt ggcgacgcag cgcgccgtgc acatctgtta
     2221 cggctacggc gtgccccagg tgatcgcctg gaaaaatcag aatcaggatt gggggcacta
     2281 cgggcacacg ctgccgctgc tgaacgagtc cagcatcgat caggtgtccg tggagtgcgc
     2341 ggcgtccggg gtcgacgtct cggtgatcca gcatctgtcc aacaagcgcg tgatgctggg
     2401 cgtgatcagc gtgagcaccg acgaggtgga aacgccggag ctggtggccg agcgcatccg
     2461 ggccggcctc gagttctgcg acgccgagcg cctcatgcct tgcacggact gcggtctggt
     2521 gccgcgcccc cgggcggtgg cccaggcgaa gatggcggct ctggctgcag gcaccgcgct
     2581 ggtgcgggac gccctctgat cgattggctc ggccgtcgcc ggccggccag ggaggcttga
     2641 ccatgtgcga cgagcaaacc gttcgtgaca acgaagcgta tctgcatacg tctgccggca
     2701 tgtcacggcg ccagttcgcg ggggccgctg caggtgcggc gattgcgatg ctgctgcccc
     2761 gctctggcaa cgccctgacg gtttcgggcc gcgacgttcg gatcaccacg ccggacggcg
     2821 aggccgatgc gtacttcgtg catcctgccg aagggaagca cccgggggta atcgtgtggc
     2881 cggacatccg gggactgcgg ccggcgttcc ggcagatggc cgaccgcctg gctggttcgg
     2941 gctacgcggt gctggtggtc aatcccttct atcgggatgc gcgcgcgccc ttggttcccg
     3001 aaggtgccag ctttcaggac gaggccaccc gtgccgtgct gatgccgctg tacaactcca
     3061 tcacgtcgac caccaccgac gtggacgcca aggcgctcgt cgactttctc gatgcccaaa
     3121 ccgaggtgga cacgacggga gggatcggga ccatggggta ctgcatgggc ggcccgatga
     3181 ccctgcactc ggcagctgcg gtcccggatc gagttgcggc ggcggcttcc tttcatggca
     3241 gcaggctcgt cacagacgcc gaggacagcc cgcacctgag agtgcccggc atgtcggcga
     3301 gctttctcat cgccattgcc gagaacgacg atgcccgaga ccctcaggcc aaggacgtcc
     3361 tcgagctcgt attcgacgat gcgggcctgg ccgccgagat cgaagtctac gaaggagcgc
     3421 tgcacggctg gtgcccgccc gactcgtccg tctaccatga agggcaggcc gagcgggcct
     3481 gggagcggtt gctggtcttg ttcgagtcag cgctgaaaag ctgatccgtc gcgccatttg
     3541 gtgcagtgaa gggactcagg tcctatactg ccgggtcctc ccctcggaat ctctagtgaa
     3601 atatctggct cgcgccattc tgcggctggg cggttggcgc atcgagggtg ccaagcccga
     3661 tcttgaaaaa tacgttctgg tggcggcgcc gcacacctcg aactgggatt ttctgtggat
     3721 gatctgcatg gctcaggcgc tggagattcc tgtgtcctgg cttggcaaag acgcgctctt
     3781 ccatcccctt attgg
//
LOCUS       DBNHKV010000284        12880 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_820896, whole genome shotgun sequence.
ACCESSION   DBNHKV010000284 DBNHKV010000000
VERSION     DBNHKV010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12880)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12880)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12880
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_820896"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="AC16PP_08935"
     CDS             complement(<1..987)
                     /locus_tag="AC16PP_08935"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6481792.1"
                     /translation="MFARHRSLVDASGRIPSWRLGIFASIAFPSVGMTLPLAIFLPPF
                     YTKTLGLGLAEVGLVFMLVRLFDIVTDPVMGIIGDRFDSRWGRRRHWLVIALPFMMIG
                     VYMAFMPTGSPSIWYLGFWLVVLYAGTTMKTISHTAWAAELSPDYDQRSRIAGFNSFA
                     AYMGSLLILGPLAFLEFRGTPPAGHEALTFFGTMALVFAPICVFAAVTLVGERPTAPA
                     PRIGLIRGLRMVLRNPHMRRLLLADAMAAIPGSVMSGLFIFYQAELLGNSQFNSLALI
                     GFFVAHIIGVPLWVRLSFRVGKHRAFGISSLCFCFTTSLFFFPGEGDVALFVA"
     gene            complement(996..2273)
                     /locus_tag="AC16PP_08940"
     CDS             complement(996..2273)
                     /locus_tag="AC16PP_08940"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6481793.1"
                     /translation="MSDPRTCPVEGVMEVDFNSEPFLTDFKQTYAAMHAGGCPYAHST
                     AGDHYAVASHAQIVAMLKRYDLWKSKHGPGLNYQERSGVLVSVDPPEHTFEVKIVASA
                     FSVPYFESLIPEMQAFVDEQIDGFHADGRVDLHQALSIALPLFVIFRMFGIPLRDEAG
                     DKTEFVREMIIGSVGLMLTPGHQVQAELAAGSVDEARMRAIAGTQQMFVDHLADCKRK
                     LESGEYHDDSNIVCRFLTTPGPDGTYLSDAKILGFCGFLLTAGSATTTIMLSNVVYRL
                     LRQPEELAKLKANPELVPLAIEESLRLDAPVQGLFRTNDEETDLGPLHLEKDTKVMLL
                     WAGANLDPTVFDDPLRFSLDRDLAKVRRHLAFGYGTHFCRGAALARIEGEIFLRTVLE
                     RLPNLRLDGEVVMEKRMPVLQGIRSLPLAWDVD"
     gene            complement(2360..3028)
                     /locus_tag="AC16PP_08945"
     CDS             complement(2360..3028)
                     /locus_tag="AC16PP_08945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6481794.1"
                     /translation="MARGYPKIDLPVGCEPTDNIWEAMFTQRAIRYWQDKPVPRELLE
                     KVVAAGSKAPSGSNTQPWVFVVVDEPEKRAELGAALRGIFDAAEPLQQLISAGEQSDD
                     KTERLMLKGARAFFSHLEKAPAMIIPCLYGLSSPTQDATSLLAGSSIYLAVQNVLLAA
                     RALGLGTVMTTVHGMLDADLRRILSLPEDATPVALLPIGYPDANFGPTTRKGLDEILR
                     WNGW"
     gene            complement(3092..4339)
                     /locus_tag="AC16PP_08950"
     CDS             complement(3092..4339)
                     /locus_tag="AC16PP_08950"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6481795.1"
                     /translation="MQLPTDEEWARACAADGEFGLAARHWTGGLTLAVGEAELSLRLT
                     DGAVSPGRAESAEGLLTYAGPADVWEQVLAAQPPRFHNDIMANLTVGGGLNRQVDPVV
                     HAQYYPAVMRAVELLRPAEPPFMPMRDEARPPGAFDAPVGRYVHLELEGHDHRIYFEE
                     AGQGIPLLMQHTAGCHGSQWRHLFEIPEITSRFRLIAYDLPYHGKSVPPVSREWWAER
                     YELKGDFLRSVPVKLAEALGLDRPVFMGCSVGGLLALDLALRHAEAFRAVISVEGALN
                     IEGDLGELEELWHPQVSNEYKARLMDGIMAPASPKAYRKETSFVYACGWPPVFLGDLY
                     YYINDFDIRQAAGQIDTSRVGVHILSGEYDYSGTSELGRQAHELIPGSTWTEMKGVGH
                     FPMTENPVRFVEYLEPILASISA"
     gene            complement(4432..5133)
                     /locus_tag="AC16PP_08955"
     CDS             complement(4432..5133)
                     /locus_tag="AC16PP_08955"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR6481796.1"
                     /translation="MKEFLSARAVEFVSINVLEDREARERLARAGIRTLPVVSRDDAF
                     VPGMDLARVAEFLGLDYQGGNILPADVLVSRYHRVLDAAVRYVGQIPPARLHDTLPHR
                     DRSYLALGNHLVQIAVDFLSVTRGAALTGRLAESVPETELEPPALMAAAAQAKRGLAD
                     WLAAADSAELASPVVTYFGSQTLHQVLERAVWHSAQHGRQLMMVLQRLGISPDGPLTD
                     EDFAGLPMPEEVWDG"
     gene            complement(5183..5629)
                     /locus_tag="AC16PP_08960"
     CDS             complement(5183..5629)
                     /locus_tag="AC16PP_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481797.1"
                     /translation="MVKASVWVVGLLVVAAGGYYAYLQLVGNPRVMTEIRAEPDGERA
                     RRVMLIDLPDGRTLPVNYLREDDMVYMGVDGLWWRQFRQGDVPVELLIRGEDLQGHAR
                     AVLDDPDRTRRVFARLRPTAPDWLPGRMGGVLVEIRLQAATLDEED"
     gene            5813..6577
                     /locus_tag="AC16PP_08965"
     CDS             5813..6577
                     /locus_tag="AC16PP_08965"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6481798.1"
                     /translation="MSGRFDGKVVMITGATSGLGKHAAVAFAREGARVVASGRRQAEG
                     EETLAEIEAAGGTAVFVQADVSVSEDVARIVAAARDRFGGLDWAYNVAGVPGDAFAKA
                     AEYSEDAWDETMEINVKGVFLCVKHAIPAMLERGGGAIVNMSSIAGIGGSPGGVAYVA
                     SKHAIIGLTKAAALDYAEQNIRINALAPGVIYTDMLAWGITQTPGLEADLISQHPIGR
                     LGQLEEVTNAAMWLCSDEAGFVTGTTLTVDGGHLAR"
     gene            6627..7889
                     /locus_tag="AC16PP_08970"
     CDS             6627..7889
                     /locus_tag="AC16PP_08970"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6481799.1"
                     /translation="MNQKEIDELRQLMEYEAARSEPPEGFPSLPDLPGGRYTDQRFYD
                     LEQQHIWRKSWLLAGHIDEIPEPGCFRLWETAGQPVVLVHADSGAVNAFYNTCSHRGA
                     PVVTEPEGKRPRLVCKYHGWTYSHDGELLAIRDPEDFRDLDFSCRGLRPIRCESFGNL
                     IFVNFDDQAPTLLDWLGPVAAEWEEFQFDKCRLASRHIFDLNCNWKIAMEANTEVYHV
                     RSIHPKTVAPMLDDRRNVNTLYPNGHGRMVAPVPPSAKVPSIVDLPSGSSIETVGEIA
                     RTCTQSYGVFPNWVSPLNHRVLPPLLFWPNGIDKCRLETWTLAPDWGNEPKPDMWTVN
                     DGESLSDVLLEDTEFGEWIQKSVESYGFKGVPLSYQEARIYHWNQFADRMIGIDNIPP
                     ELRVAQVIGEEWVWPNEPRLALARASAG"
     gene            7929..8423
                     /locus_tag="AC16PP_08975"
     CDS             7929..8423
                     /locus_tag="AC16PP_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6481800.1"
                     /translation="MSDQAFTPEEIAELRNLLEIEKIRKVKLLYSQLMDSRDIDALAD
                     IFAEDAICEFGPYGTWHGQDEIRANYHQVFKDAAPYDGIHATTNQWVELTSPTTAVSR
                     TYLIDVSHEANPRVNPVVWYGLYDEDYEKIEGAWKVKRCSLQFLWPERLVNEGWPGPF
                     PPAS"
     gene            complement(8461..8670)
                     /locus_tag="AC16PP_08980"
     CDS             complement(8461..8670)
                     /locus_tag="AC16PP_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481801.1"
                     /translation="MKGMKHPFGRALYEQDGNGNVLVTTVDGVWGRFRTDGSWLEGEL
                     KEADPHMCGWVGGPTVAHHRIQVEE"
     gene            complement(8673..10013)
                     /locus_tag="AC16PP_08985"
     CDS             complement(8673..10013)
                     /locus_tag="AC16PP_08985"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6481802.1"
                     /translation="MSEAVDARSPGTTYQELLDTDSHAVPDVLRLESPAPRRLNDLSI
                     DRYTSRDFHDREMEKLWSRVWQFACREEHIPNPGDYLVYEIGRHSYVVVRSHQGAIKC
                     FVNACLHRGRMLKHYDGNCTELRCPFHGFAWETDGRLKDIPASWDFPQIVASEFSLPE
                     AQVATWAGFVFVNPDPDAAPFREFLGGMAAHFDRWNLADRYVQVHVIKEIRANWKIAQ
                     EAFCEAFHVNATHPQTMPYLGDTNSQVDVWDTFSRVITPGGTPSPLLDWEPDESDILR
                     HMLDIREDMELPWSLEGTTARKSAADSARERWRPVVGGLVDEWSDAEFIDNLDYTLFP
                     NFHPWGAFNRIVYRFRPNGDDHRSSLMEVMMLAPFTGERPPPADVVRLDADQPWTDAS
                     ELGMLAKVFEQDTFNMGRVQRGLETTRKPGVTLAGYQEAKVRWLNETLDEWLER"
     gene            complement(10087..11547)
                     /locus_tag="AC16PP_08990"
     CDS             complement(10087..11547)
                     /locus_tag="AC16PP_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6481803.1"
                     /translation="MTKGYQVRLPTKIVYGLGSVSDGVKNTTFNVFLLFFYTQVAGLS
                     GSLAGAAILIALVFDAVSDPLIGNISDNFHSRWGRRHPFMYAAALPMAVFFGLIFSPP
                     QGLGQAGLFAWMLTMAIGVRVSMTLYAIPSGALVAEMTSHYDERTSLVSFRVLSGWLG
                     GLVASQMGYLVFFAPSAAYADGRLDPQAYGDYAVFGAITIFCAILICAIGTHRLIPEL
                     KSPPAHTPFTLRRFGVELREVFSNRPYLVLVFTVLVVSTATGFTDVMGLYVNTYFWEF
                     TTAQLAIIVFGALAGVVAAFSVTATLAQRTDKRTMALAMLLVVVIIGPLPVVLRLLGL
                     MPANGDPLLLGIMIAWTGVIVFVAVGVTILAGSMIADTVDLNELRTGKRQEGMFSAAM
                     GLTAKATSGIGGFVGGVVLDVVDFPTGVAAGEVDADTLFALGVAVGPGMFLLWITVLI
                     ILSRYRLSRADHADILGKLEARRNAAEQPGGEAAGG"
     gene            11797..12408
                     /locus_tag="AC16PP_08995"
     CDS             11797..12408
                     /locus_tag="AC16PP_08995"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6481804.1"
                     /translation="MKFYNSIGPNPQVVRTFAAERGIELDMEEVDLMAADNRREPYLS
                     KNPAGQSPCLELDNGDYVSEVTAICEYLDETSPGTSLIGSTPEERAETRMWVRRIDLN
                     ICEPLGYGFRFGEGLDFFKDRITTLPEASEGMKRIAAEKIAWLDGLMAGREFVCGDRI
                     TLADVLLYCFLAFGKTVGQPYAETNQNIHAWFERMSQRPSVSA"
     gene            12426..>12880
                     /locus_tag="AC16PP_09000"
     CDS             12426..>12880
                     /locus_tag="AC16PP_09000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6481805.1"
                     /translation="MRFSLMLPVFVDAGAASPFERTLGFARTADELGYHMGTFGHHSF
                     TPQIADPSAPFALLSAVAARTERLRLGTGVYLAPLHHPVTVAEQAAMLDQISGGRAVL
                     GVGLGYRPYEYEGFGLDFHTRGKRLDELLTAIREGWTRGEFRHDGEHFR"
BASE COUNT         2361 a         4302 c         4250 g         1967 t
ORIGIN      
        1 cgccacgaac agcgcgacgt cgccctcgcc cgggaagaag aacagcgacg tggtgaagca
       61 gaagcacagc gagctgatgc cgaacgcgcg gtgcttgccg actctgaacg acaggcgtac
      121 ccagagcggc acgccgatga tgtgagccac gaagaagccg atgagcgcga gcgagttgaa
      181 ctgggagttc cccagcagct ccgcctgata gaagatgaac agccccgaca tcaccgaacc
      241 gggaatggct gccatggcat cggcaagcag caggcggcgc atgtgggggt tccgcagaac
      301 catgcgcagg ccgcgaatca gcccgatgcg aggcgccggc gccgtggggc gctcgccaac
      361 cagggtgacc gccgcaaaca cgcagattgg cgcgaacacc agcgccatgg tgccgaagaa
      421 cgtcaacgcc tcgtgccctg ccggcggcgt tccccgaaat tcgagaaacg ccagggggcc
      481 caggatgagc agcgagccca tgtaggccgc aaacgagttg aagccggcga tgcgcgatcg
      541 ctggtcgtaa tcgggggaca gctccgccgc ccacgccgtg tgggagatgg tcttcatggt
      601 cgtgcctgca tagagcacca ccagccagaa gcccagatac cagatcgagg gcgacccggt
      661 cggcatgaac gccatgtaga cgccgatcat catgaagggc agcgcgatca ccagccagtg
      721 ccggcgccgg ccccagcggc tgtcgaagcg gtcgccgatg atgcccatca ccgggtcggt
      781 gacgatgtcg aacaggcgca cgagcatgaa cacgagaccc acctcggcaa ggcccaggcc
      841 cagggtcttt gtgtagaacg gcggcaggaa gatggccagc ggcagcgtca tgccgacgct
      901 ggggaacgcg atggaggcga agatgcccag ccgccaggag gggatgcgcc cggaagcgtc
      961 gacgagagac cgatggcggg cgaacatggg ccgcgtcagt ccacgtccca cgccagcggc
     1021 agggagcgga ttccctgcag caccggcata cgcttctcca tgaccacctc cccgtccagc
     1081 ctcagattgg gcagccgctc cagcaccgtg cgcaggaata tctcaccctc gatccgggca
     1141 agcgccgcgc cgcggcagaa gtgggttccg tacccgaagg ccagatggcg gcggaccttg
     1201 gcgagatccc ggtccaggct gaaacgcagc gggtcgtcga agaccgtcgg gtcgaggttg
     1261 gcgcccgccc acagcagcat gaccttggtg tccttctcca ggtgcagagg tcccaagtcc
     1321 gtttcctcgt cgttggtgcg aaacaggccc tgcacgggcg cgtcgaggcg cagggactct
     1381 tcgatggcaa gcggcacgag ctcggggttg gccttcagct tggcgagctc ctcgggttgc
     1441 ctgagcaggc ggtagacgac gttggacagc atgatcgtgg tcgtcgcgct ccccgccgtc
     1501 agcagaaagc cgcagaaacc gaggatcttc gcatccgaca ggtaggtgcc gtccggaccc
     1561 ggcgtggtca gaaaacggca gacgatgttg ctgtcatcgt ggtactcgcc ggattcgagc
     1621 ttgcgtttgc agtccgccag atggtctacg aacatctgct gggtaccggc aatggctctc
     1681 attcgggcct cgtccacgga cccggcggcg agctcggcct gcacctggtg gccgggcgtc
     1741 agcatcagcc cgacggagcc gatgatcatc tcgcgcacga actccgtctt gtcgccggcc
     1801 tcgtcgcgca gcgggatgcc gaacatccgg aagatcacga acagcggcag cgcgatggac
     1861 agcgcctgat gcagatccac ccgcccatcg gcatggaaac cgtcgatctg ctcatccacg
     1921 aacgcctgca tctcgggtat gagggactcg aagtagggca ccgagaaagc gctggcgacg
     1981 atcttcacct cgaaggtgtg ctccggcgga tccacgctga cgagcacgcc cgagcgctcc
     2041 tgatagttga gaccggggcc gtgcttgctc ttccaaagat cgtagcgctt gagcatggcg
     2101 acgatctggg catgggacgc cacggcgtag tgatctcccg cggtggaatg cgcgtaaggg
     2161 cagccgcccg cgtgcatggc ggcgtaggtc tgcttgaagt cggtgaggaa gggctcggag
     2221 ttgaaatcca cctccatgac cccttccacc ggacaggtcc tgggatcgct catggggtgc
     2281 ccccggcgcg caccgcggcg ccgcggtcac ggggccggcg cagccgcagc cgccgacggg
     2341 tagccagggg cctcacgatc taccagccgt tccagcgcag gatctcgtcg agccccttgc
     2401 gggtcgtggg tccgaagttg gcgtcggggt agccgatggg gagcagcgcc acgggcgttg
     2461 cgtcctcggg cagcgacagg atccgccgca gatcggcgtc gagcatcccg tggacggtgg
     2521 tcatcacggt gccgagccca agcgcccgag ccgccagcag cacgttctgc acggccaggt
     2581 agatcgagct tccggccagc agcgacgtgg cgtcctgagt gggcgaggac aagccgtaga
     2641 ggcagggaat gatcatggcg ggcgccttct ccagatggga gaaaaacgct ctggcgccct
     2701 tgagcatcag ccgctcggtc ttgtcgtcgg attgctcccc ggcggagatc agctgctgca
     2761 agggctccgc tgcgtcgaaa atcccccgca gtgctgcccc gagctcggcc ctcttttcgg
     2821 gttcgtcgac caccacgaac acccacggct gggtattcga gccgctcggc gccttggacc
     2881 cggcggccac gactttctcc agcaattctc tgggaacggg cttgtcctgc cagtagcgga
     2941 tggcccgctg ggtgaacatg gcctcccaga tgttgtcggt cggctcgcaa cccaccggca
     3001 gatcgatctt aggatatcct ctcgccatgc tccccccttc cctgctcggc cggatggttc
     3061 agagtagagc agttttccag cgggaatgaa actaagccga gatcgaggcg agtatcggtt
     3121 ccaggtactc gacgaaccgc acgggattct cggtcatggg gaagtggccg acccccttca
     3181 tctccgtcca ggtggaaccc gggatcagct cgtgggcctg ccggcccagc tcggaggtgc
     3241 cgctgtagtc gtactcgccg gagaggatgt gcacgcccac ccggctggtg tcgatctgcc
     3301 ccgctgcttg ccggatgtcg aagtcgttga tgtagtagta cagatcgccc agaaaaaccg
     3361 gcggccaccc gcaggcgtaa acgaagctcg tttccttgcg gtaagccttc ggcgaggcgg
     3421 gcgccatgat cccgtccatg aggcgggcct tgtactcgtt gctcacctga gggtgccaga
     3481 gctcctccag ctcgcccaga tcgccctcga tgttgagcgc gccttccacg gagatcacgg
     3541 cccggaacgc ctcggcgtgt cgcagcgcga ggtccagagc cagcagcccg cccacggagc
     3601 atcccatgaa aacgggccgg tccaaaccga gcgcctcggc cagcttgacc ggcaccgagc
     3661 gcaggaaatc tcccttgagc tcgtagcgct ccgcccacca ctcgcggctg acggggggga
     3721 ccgacttgcc gtgatacggc aggtcgtagg cgatgaggcg gaagcggctg gtgatctcgg
     3781 gaatctcgaa gaggtggcgc cactggctgc cgtggcagcc ggccgtgtgc tgcatcagca
     3841 gggggatgcc ctgccccgcc tcctcgaagt agatccggtg atcgtggccc tccagctcca
     3901 gatggacata gcgccccacg ggggcgtcga aggccccggg cggtcgggcc tcgtcgcgca
     3961 tgggcatgaa cggcggctcc gccggccgaa gcagctccac ggcgcgcatc actgccgggt
     4021 agtactgagc gtgaaccacc gggtcgacct ggcggttgag cccgcccccc acggtcaggt
     4081 tggccatgat gtcgttgtga aaccgcggcg gctgggccgc gagcacttgc tcccatacgt
     4141 cggcagggcc cgcgtaggtc agcagacctt cggccgattc ggccctgccc ggagacacgg
     4201 cgccgtccgt caggcgaagg ctgagctccg cctccccgac ggcgagggtg agaccccccg
     4261 tccagtgccg ggccgccaga ccgaactcgc catccgccgc gcacgcgcgg gcccattcct
     4321 cgtcggttgg aagctgcatt cccccgttct cctccccgtt cgactcgccg ctgggcgacc
     4381 gtcaccggac gcccgccatc atcataaccc cacgcaccac cgccgcggcg gtcagccgtc
     4441 ccagacctcc tccggcatgg gcagccctgc gaaatcctcg tccgtcagcg gcccgtccgg
     4501 cgagatccca agccgttgca gaaccatcat cagctgacgc ccatgctggg ccgagtgcca
     4561 caccgcccgt tccagcacct gatggagcgt ctgggaaccg aagtacgtca ccacaggtga
     4621 agcgagctcg gcactgtccg ctgccgccag ccagtccgcc aggccgcgct tcgcctgggc
     4681 ggcggcggcc attagtgctg gcggctccag ctcagtctcc ggcaccgact cggcaagccg
     4741 gccggtgagc gcagcacccc gggtcaccga caggaaatcc accgcgatct ggacgagatg
     4801 attgcccagg gcaaggtagg aacggtcccg atggggcagc gtgtcgtgca ggcgggcggg
     4861 cggtatctgc cccacgtatc gcaccgcggc atcgagcacc cggtgatacc ggctgacgag
     4921 cacgtctgcc ggcaggatgt tgccgccctg atagtccagg cccaggaatt cggcgacccg
     4981 cgcgagatcc atcccgggca cgaaagcgtc gtcgcgggaa accacaggca gggtccggat
     5041 acccgcacgg gcaaggcgct cacgcgcttc ccggtcctcc agcacgttga tggaaacgaa
     5101 ctcgaccgca cgggccgata gaaactcctt cacccggaga cagctggttc acccgggctg
     5161 ccagtagacg caggtcccca cgtcagtcct cctcatcaag agtcgccgcc tgcagccgga
     5221 tctcgaccag cacgccgccc atccggcccg gaagccagtc cggcgcggtg ggtcggagcc
     5281 gggcgaagac cctgcgggtc cggtcgggat cgtcgagcac ggcacgggcg tggccctgca
     5341 gatcttcacc acggatgagc agctcaacgg gcacatcgcc ctggcgaaac tgccgccacc
     5401 acaacccgtc cacccccatg tagaccatgt cgtcctcacg caggtagttg accggcaggg
     5461 tccggccgtc gggcaggtcg atgagcatga cgcgccgtgc ccgctcgccg tccggctcgg
     5521 cccggatttc ggtcatcacc cggggattgc cgacgagctg caggtacgca tagtagcctc
     5581 cggccgcaac gaccagcagc ccgacgaccc acaccgacgc tttgaccact ttcggcaagt
     5641 ccgacccgcc ccgggaactc agagcacgta gcctgacaca gggcagcgca aagctgaacc
     5701 gccgggcgct ccggcagact ctcgcccggg gccgtggaat ccggccttga tgagatagcg
     5761 ggtacgcagg gcctcgggca caattggcgc cagagtgctt ggggagcaaa cgatgagcgg
     5821 acgcttcgac ggcaaggtcg tcatgatcac cggcgccacg tccggtctgg gtaagcacgc
     5881 ggccgtcgcc ttcgcccggg aaggggccag ggtcgtagcg tccggacgac gccaggcgga
     5941 aggcgaagag accctcgcgg agatcgaagc cgccggcggc acggccgtgt tcgtccaggc
     6001 cgacgtcagc gtgagcgagg acgttgcgcg catcgtggcc gctgcccggg accgattcgg
     6061 cgggctcgac tgggcctaca acgtggccgg cgttcccggc gacgcctttg ccaaggcggc
     6121 agagtattcc gaggacgcct gggacgagac catggagatc aacgtcaaag gcgtttttct
     6181 ctgcgtaaag cacgccatcc cggccatgct cgagcgcggg ggcggcgcca tcgtcaacat
     6241 gtcgtccatc gcgggaatcg gcggctcgcc gggcggcgtg gcttacgtgg cgtccaagca
     6301 cgccatcatc gggctcacca aggccgcagc gctggactac gccgagcaga acatcaggat
     6361 caacgccctg gcgccgggcg tgatctacac cgacatgctc gcctggggaa tcacccagac
     6421 gcccggcctg gaggccgatc tgatctcaca gcatccgatc gggcggctgg ggcagctaga
     6481 ggaagtgaca aacgcggcca tgtggctgtg ctcggacgag gccggcttcg tcacgggcac
     6541 caccctgacc gtggacggcg ggcacctggc ccgctaaaac gaagcatcgt agaaccatcg
     6601 atggcacgac gagagaggct gaatccatga accagaaaga aatcgacgag ctgcgccagc
     6661 tcatggagta cgaggcggca cgaagcgaac cgcccgaagg ttttccgtca ctgcccgacc
     6721 tgccgggcgg tcgttacacc gatcagcgct tctacgacct ggagcagcag cacatctggc
     6781 gaaagtcgtg gctgctcgcc ggtcacatcg acgagattcc ggaacccggc tgtttccgcc
     6841 tctgggagac cgcgggccag cccgtggtgc tcgttcatgc ggacagcggc gcggtgaacg
     6901 ccttctacaa cacgtgcagc caccgcggcg cgcccgtcgt caccgaaccg gaaggcaaac
     6961 gcccgcgtct ggtgtgcaaa tatcacggct ggacctacag ccacgacggc gagcttctgg
     7021 ccatccgcga tcccgaggac ttccgcgatc tggatttctc ctgccgcggc ctgcggccca
     7081 tccgctgcga gagctttggc aacctgatct tcgtgaactt cgacgaccag gcccccacgc
     7141 tgctggactg gctgggcccg gtggccgcgg agtgggagga gtttcagttc gacaagtgcc
     7201 ggctcgcctc ccgccacatc ttcgacctga actgcaactg gaagatcgcc atggaggcga
     7261 acacggaggt ctaccacgtt cgcagcatcc acccgaagac cgtcgcgccc atgctcgacg
     7321 atcgccgcaa cgtgaacacg ctgtatccca acgggcacgg acggatggtt gcgccggtgc
     7381 cgcccagcgc caaggtgccg tccatcgtcg acctgccgag cggcagcagc atcgagaccg
     7441 tgggcgagat cgcccgcacc tgcacccagt cctacggcgt ctttcccaac tgggtgtcac
     7501 ccctgaatca ccgggtgctc cctcccctgc tgttctggcc caacggcatc gacaagtgcc
     7561 ggctggagac ctggaccctg gcgccggact gggggaacga gccgaaaccc gacatgtgga
     7621 cggtgaacga cggggagagt ctcagcgacg tgctgctgga ggacaccgag ttcggcgagt
     7681 ggatccagaa gtcggtggag tcctacggct tcaaaggggt cccgctgagc taccaggagg
     7741 ccaggatcta ccactggaac cagttcgccg accgcatgat cggcatcgac aacattccgc
     7801 cggagctgcg cgtggcccag gtgatcggcg aggagtgggt ttggccgaac gagccccgac
     7861 tcgcgctcgc ccgggcaagc gccggctgac ggccatcggg cgcgcactca acacaggagc
     7921 acgaagagat gtctgaccag gcctttaccc ccgaggagat cgcggagctg agaaatctcc
     7981 tggagatcga gaagattcgg aaagtgaagc tgctctactc gcagctgatg gacagccgcg
     8041 acatcgacgc cctcgccgac atctttgccg aggacgcgat ctgcgaattc gggccctacg
     8101 gcacctggca cggccaggac gagatccgcg cgaactacca ccaggtattc aaggacgccg
     8161 cgccctacga cggcatccac gccaccacca accagtgggt ggagctgacc agtcccacca
     8221 cggcggtgag ccgcacgtac ctcatcgacg tcagccacga ggccaacccc cgggtgaacc
     8281 cggtggtctg gtacggtctt tacgacgagg actacgagaa gatcgaaggc gcctggaagg
     8341 tgaagcgctg cagcctgcag tttctctggc cggagcggct cgtcaacgag ggctggccag
     8401 ggccgttccc gccggccagc tagcccgacc ggtgcgggcg cgacgctggg gcggcgccgc
     8461 tcattcctcc acctgaatcc gatggtgggc caccgtgggc ccgcccaccc agccgcacat
     8521 gtgcgggtcg gcctctttga gctcgccctc gagccagctg ccgtcggtac ggaaccgccc
     8581 ccacacgccg tccaccgtgg tcaccagcac gttgccgttg ccgtcctgct cgtacagggc
     8641 ccgaccaaac ggatgtttca tgcctttcat gttcagcgct ccagccattc gtccagggtc
     8701 tcgttgagcc agcgcacctt ggcttcctga tagccggcga gcgtgacgcc cggcttgcgc
     8761 gtggtctcca ggccccgctg aacgcgcccc atgttgaacg tgtcctgctc gaacaccttg
     8821 gccagcattc ccagctccga tgcgtccgtc cacggctggt cggcatcgag gcggacgacg
     8881 tccgccgggg gcgggcgctc accggtgaag ggcgccagca tcatcacctc catcagcgag
     8941 gaccggtgat cgtcgccgtt ggggcggaag cggtagacga tgcggttgaa ggcgccccag
     9001 gggtgaaaat tgggaaacag ggtgtagtcc aggttgtcga tgaactcggc gtccgaccac
     9061 tcgtccacca gaccgccgac cacgggccgc catcgctctc gcgcggaatc ggccgccgac
     9121 ttgcgcgcgg tggttccctc caaggaccag ggcagctcca tgtcctcgcg gatgtccagc
     9181 atgtgccgga ggatgtcgct ctcgtcgggc tcccagtcga gcagcgggct gggggtgccg
     9241 cccggcgtga tcacccggga aaacgtatcc cacacgtcca cctggctgtt ggtgtcgccc
     9301 agatagggca tggtctgcgg gtgcgtggcg ttgacgtgga aggcctcgca gaaggcctcc
     9361 tgagcaatct tccagttcgc ccgaatctcc ttgatcacat ggacctgcac gtagcggtcg
     9421 gcgaggttcc agcgatcgaa gtgggcggcc atgccgccca ggaattcccg gaacggcgcc
     9481 gcgtccgggt cggggttgac gaagacgaaa ccggcccagg tggctacctg ggcttccggc
     9541 agagagaact cggaggcgac gatctggggg aagtcccagc tcgccggtat gtccttcagc
     9601 cgcccgtcgg tttcccaggc gaagccgtga aacgggcagc gcagctcggt gcagttgccg
     9661 tcgtagtgct tgagcatgcg gccccggtga aggcacgcgt ttacgaagca tttgatcgcg
     9721 ccctgatgag aacgcaccac cacgtaagag tgccggccga tctcgtagac caggtagtcg
     9781 cccgggttgg ggatgtgctc ctcgcggcag gcgaactgcc agacccgcga ccagagcttc
     9841 tccatctccc gatcgtgaaa gtcgcgggag gtgtagcggt cgatggacag gtcgttgagc
     9901 cggcgcggcg ccgggctctc cagccgaagg acatccggca cggcgtggga gtcggtgtcg
     9961 agcagctcct gataggtcgt tcccggcgag cgggcgtcga cggcctctga catggcggcc
    10021 ttcctttgat ctgagtgacc gccgagtgtg ccaccggcgc tgggcggaat gcaaagcagc
    10081 gctcggctag cccccggcgg cttccccgcc gggctgctcg gcggcgttcc ggcgggcttc
    10141 gagcttgccc aggatgtccg cgtgatcggc ccggctcagc cgatagcgcg acaggatgat
    10201 caacaccgtg atccagagca gaaacatgcc ggggcccacg gccacgccca gcgcgaagag
    10261 ggtatccgca tccacctcgc ccgcggccac gcccgtcgga aagtcgacca cgtccaggac
    10321 cacaccgcca acgaagccgc cgatgcccga tgtcgccttg gccgtcaggc ccatggccgc
    10381 gctgaacatg ccttcctggc gtttgcccgt gcgcagctcg ttgaggtcca cggtgtcggc
    10441 gatcatggag ccggccagaa tcgtcacgcc caccgcgacg aacacgatca cgcccgtcca
    10501 ggcgatcatg atgccgagca gcagcggatc gccgttcgcc ggcatgaggc ccagcagccg
    10561 cagcaccacg ggcagaggtc caatgatcac caccaccagc agcatcgcca gcgccatggt
    10621 gcgcttgtcc gttcgctgcg caagcgttgc cgtgaccgag aaggccgcta ccacgccggc
    10681 cagcgcgccg aagacgatga tggcaagctg cgcggtggtg aactcccaga agtacgtgtt
    10741 cacgtacaga cccatgacgt cggtgaagcc ggtggccgtg gagacgacga gcaccgtgaa
    10801 cacgagcacg agatacgggc ggttgctgaa cacctcgcgc agctcgacgc cgaaccggcg
    10861 cagggtaaac ggtgtatgcg cgggcggcga tttcagttcg ggaatcaatc gatgggtgcc
    10921 gatcgcgcag atcaggatgg cgcagaagat cgtgatggcg ccgaagacgg cgtagtcgcc
    10981 gtaagcctga ggatccaggc gaccgtccgc gtaggcggca gacggggcaa agaagacgag
    11041 gtagcccatc tgggaggcga ccagccctcc cagccagccg gagagcaccc ggaagctgac
    11101 cagggacgtt cgctcgtcgt agtggctcgt catctccgcc accagcgcgc cgctgggaat
    11161 ggcgtacaac gtcatggaca cccggacacc aatggccatg gtcagcatcc aggcgaagag
    11221 tccggcctgg cccagccctt gcggcgggct gaagatcagc ccgaaaaaga cggccatggg
    11281 cagcgcggcg gcatacatga acggatggcg gcggccccag cgggagtgaa agttgtccga
    11341 gatgttgccg atgagagggt cgctgaccgc atcgaacacc agcgcgatga ggatagccgc
    11401 accggccaac gagccggaga ggcccgcaac ctgggtgtag aagaagagca gaaaaacgtt
    11461 gaacgtggtg ttcttgacgc cgtcggaaac gctgccaagc ccgtagacga tcttggtggg
    11521 aagcctgacc tgatagcctt tcgtcacggg accttccacg atgcctcaat atcacggccc
    11581 cgccgggggc ggacccgggc acacggctat cggcagaatc cgggctccgg ggaaggtcgg
    11641 cgagtctacg cgcctggaac ggtgcttgca cctgcccgcg cggcccgtac aacccgggca
    11701 tcagcctcca ggccggcggc gcgccggtcg cccgtggtgg tgcgcagcgg cgccggcccc
    11761 tagaattcga cgtttgaatc aatcgggtaa cacgcaatga aattctacaa ctccatcggc
    11821 ccgaaccctc aggtggtgcg cacgtttgcc gccgagcgcg gcatcgaact cgacatggag
    11881 gaggtcgacc tcatggcggc cgataaccgc cgggagccct acctgtcgaa gaacccggcc
    11941 ggccagtcgc cgtgcctcga gctcgacaac ggcgactacg tcagcgaggt cacggcaatc
    12001 tgcgagtatc tcgacgagac gagcccaggc acgtctctga tcggcagcac gccggaggag
    12061 cgcgccgaaa cgcgcatgtg ggtgcgccgc atcgacctga acatctgcga gcccctgggc
    12121 tacggcttcc gcttcggcga gggattggat ttcttcaagg accgcatcac caccctaccg
    12181 gaagcttccg aaggcatgaa gcgcatcgcg gcggagaaga tcgcctggct cgacggcctc
    12241 atggccggcc gcgagttcgt ctgcggcgac aggatcacgc tggccgacgt gctgctgtac
    12301 tgcttcctcg ctttcggcaa gaccgtgggc cagccctacg cggagaccaa tcagaacata
    12361 cacgcgtggt tcgagcggat gtctcagcgg ccaagcgtgt cggcgtgatt cccgagtaag
    12421 cgcccatgag gttcagtctc atgctgccgg tgttcgtgga cgccggggcc gcgagcccct
    12481 tcgaacgaac actgggcttc gcccgcaccg cggacgagct cggctaccac atgggcacct
    12541 tcggccatca ctcgttcacg ccacagatcg ccgatccgtc cgccccgttt gcgcttctgt
    12601 cagcggtggc ggcgcgtacc gagcggctgc gcctgggcac cggcgtctac ctcgccccgc
    12661 tgcaccatcc ggtgaccgtt gcagagcagg ccgccatgct ggaccagata tccggcgggc
    12721 gcgcggtgct gggggtcggc ctcggctacc ggccctacga gtacgagggc ttcgggctcg
    12781 atttccacac ccgcggaaag cggctggacg agctgctcac cgcgatcaga gaaggatgga
    12841 cgcggggcga atttcgccat gacggcgagc acttccgcat
//
LOCUS       DBNHKV010000285         8774 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1811125, whole genome shotgun sequence.
ACCESSION   DBNHKV010000285 DBNHKV010000000
VERSION     DBNHKV010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8774)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8774)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8774
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1811125"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="AC16PP_09005"
     CDS             complement(<1..1310)
                     /locus_tag="AC16PP_09005"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6481806.1"
                     /translation="MSSIRQMRGIDRTKATEPELGSNPINGDRYRSREFAELEWEHVW
                     TRVWLIGGLVHQIPEPGDFFTTDIGRESILFVRGDDGSIRAFYNVCQHRGNQLVQAEQ
                     GSLSGGEFQCAYHGWRFDAQGVLTWVYCEEDFPQGNPCGKRNLIEIPCATWAGFIWYN
                     MDPDCKPLADFLDPVARQLDNYRMEDMRRTHWVTLEGNFNWKCVQDNFNESYHLPYVH
                     PQSCISLDEHYSSCQFDLYPSGHCRMFMPGGGPNSNYQGSPEAVFERLGQDLAFWEID
                     PEPFRDDLAGLRTALQQQKRKLAQKKGYDFSRYSDEQLTDNYHYTLFPNLSFSMKPDG
                     CIFLRGNPHPTDPEKCLFDMWYLTLFPEGAKEYYSNSMRDWVSVETPAAHERGKVGEV
                     SCGPGIDQDVAIWSTQQKGLTSRGFRGEYMPTQERRIRFFHENI"
     gene            complement(1413..2321)
                     /locus_tag="AC16PP_09010"
     CDS             complement(1413..2321)
                     /locus_tag="AC16PP_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6481807.1"
                     /translation="MSDRRPAVSLAAVPGRRNTIVELAAEIERRGFAGIYCPSMGDGL
                     ALCEAIAYNTRQIEFGTSITPIYTRHANDFASTAAFIHEISNGRFRFGLGVSHAPAME
                     RLGVRQGKPLSDMRQFVSDLRAAPRVGELPPVVLATLRDRMIGLAAEVAEGMVFANAA
                     RGHMQHSLNVLGDERRADPGFFIGNMIPTCISDDVEAAKAVNRRTLTGYAMLPNYRNY
                     WKEAGFVEEMGAVEKALEAGEPDRIPDCLSDAWLAETTLFGPANAVRDGIEAWFDAGV
                     RTPIIVPSSAVGNQLKAFEEFFEIWS"
     gene            complement(2430..3191)
                     /locus_tag="AC16PP_09015"
     CDS             complement(2430..3191)
                     /locus_tag="AC16PP_09015"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR6481808.1"
                     /translation="MFPLRDENPRVHPPYATVAIIILNVLVWVAVQGFGNHYALAESL
                     CLYGLIPGDLLGNAVPGTSIPLGSNLVCQLDGTANPASLFTSMFMHGGWFHIIGNMWF
                     LWVFGDNVEDVMGPFRFVVFYVVCGLAAAFAQIATNTSSLVPMVGASGAIGGVMGAYA
                     LMFPRAHVHTLIFLGFYVTTVAVPAVFMLGYWFLLQLLQGLPAMGSSQGGVAFWAHIG
                     GFLAGVALIHLFRRPNHLAQHLAQPERRSSRHRWF"
     gene            complement(3311..4612)
                     /locus_tag="AC16PP_09020"
     CDS             complement(3311..4612)
                     /locus_tag="AC16PP_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR6481809.1"
                     /translation="MTPKAGSYFPSQAASTLYAMAGDDCSLHFDLNTLTEKQLADHVS
                     NIELTAMLQVALLYVGKPVDHVALFDEFQRPPWRETLDLYRRAFTEMGVPPVPESNCH
                     YYPRDGFYEAVAADRVDADLTTLYLVSGSNAVVHRSDAALSVSQRVNSKMQLAADAAQ
                     WGIPVPETLVCRKRDLDGPEVSGFFDTHHNQIMLKLKGLAGARNVTSVAGVPECMDYV
                     AEYPNDMEIVLQRKLSADEFTEMTVDLIVRDDSIEIANVRRILFADGLWVGNLIGGGV
                     TVDPAHERALLRVGEYVRDLGYRSPEGFNCGIDYFVSADELLVTEINARWTGGLFPAE
                     MIRRLDLAGRGAVAFFDMVSRDGLPRYLDFSEQTLYRRQDEPFASVPLGFSPFPMPVD
                     GRDMHPVWQMVVGDIEAFKAAKREALEPGDLPTADKISLTF"
     gene            complement(4707..5795)
                     /gene="hisC"
                     /locus_tag="AC16PP_09025"
     CDS             complement(4707..5795)
                     /gene="hisC"
                     /locus_tag="AC16PP_09025"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR6481810.1"
                     /translation="MTHERQNIRAMHGYTWGEQPERADVIKLNTNENPYPPSPAVDRA
                     LADLSGEALRAYPQPTADRLRDQIAELHNLKRENIVVTNGGDEGLRLAFTTFLEPGGT
                     FGMADPSYSLYPVLAAIQDTRVLSIALDENWDLPRDFAHQLNDAGAGLACLVNPHAPS
                     GSLLDTSRISQLANDFNGVLLIDEAYVDFVDPALRYDAVRLTEAFDNLLILRTFSKGY
                     SLAGMRLGYLVGCASLIDPVIGKTRDSYNVDYVSQRVGEAALSDPAYAEETWANVRAG
                     RRTLREGLLEHGFRVGASQTNFLLTEVPAASPCSAKQLYEALKTRGVLVRYFDTPSLR
                     DTLRITVGTPEQNQTLLTWVGDILSRGE"
     gene            complement(5792..6838)
                     /locus_tag="AC16PP_09030"
     CDS             complement(5792..6838)
                     /locus_tag="AC16PP_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR6481811.1"
                     /translation="MGSTSKTLIVVATLIVIAAGIKAAEALMVPFLLAAFIATIAATP
                     VFWLERRRVPAGIAIALVMLGIIVMITGVGAVVAQSAGAFTNRLPFYQDRLTELVENA
                     VTYVEMAGVNISEEALLSYFDPGTALVMAGNTLRGLGGALSNGFLILLTVIFILAEAS
                     SFPRKLQEVLDDPDSDLPHFVRFAENVNRYIGIKTTISIITGVLVGLMLAVLGVDFPA
                     LWGLLAFLLNYVPTIGSLIAAIPAVLLALIQLGPLTAAVAGAGFFVINLLMGNVVEPR
                     YMGRGLGLSTLVVFVSLVFWGWMLGPVGMLLSVPLTMTAKIAMEANPSSQWLAVLLGP
                     AERAADPTPAEDQT"
     gene            complement(6865..7710)
                     /locus_tag="AC16PP_09035"
     CDS             complement(6865..7710)
                     /locus_tag="AC16PP_09035"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR6481812.1"
                     /translation="MNQGHRLTRVGVVQLCATPDVTANMIAVERLVRQACDAGAEVVL
                     LPEAFAFLGPEKAKRGILERLPDAPSAPGGPILEQCRSMAARLGCHLILGGFHEDAGD
                     PDKSYNTCVHLSPGGELLARYRKVHLFDVALADGTQLRESARTLAGHDLVTTDMPFGK
                     LGLSICYDLRFPYLYQSLVDAGAVAMTVPSAFTKTTGAAHWHVLLRARAIESQSYVIA
                     PAQHGHNWGKRFSYGHSLIVDPWGDIVAECRDGDGVAVADIDPERVRRVREELPSLTH
                     RRLLE"
     gene            complement(7753..>8774)
                     /locus_tag="AC16PP_09040"
     CDS             complement(7753..>8774)
                     /locus_tag="AC16PP_09040"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6481813.1"
                     /translation="VTGQAQTGTGKTAAFLVTIIARLWEYPPAAAPKIGSPRSLVLAP
                     TRELALQIESDAKDLTKYIDLGVECVVGGMDFQKQLDRIKRRPVDVLIATPGRLLDFI
                     SRRHLSLRDVEVLVIDEADRMLDMGFVPDVRRIVYQTPHKRNRQTLFFSATFNDDVMR
                     LANSWTIDPEHVAITPESVATDTVTQKFWLVAADKKASLLAEFLTRHAPERALIFTNR
                     RDQTHQLHAFLNKRNIRCEALAGDVPQRKRLSTLSRFKDGDIPYVVATDVAGRGIHVE
                     AISHVINFDLPEDPEDYVHRIGRTGRAGAEGVSISFVSEDDAFNLPSIEEYLGEAVVC
                     TQPSF"
BASE COUNT         1539 a         2720 c         2975 g         1540 t
ORIGIN      
        1 tcgatattct cgtggaaaaa gcgtatgcgg cgttcctggg tgggcatgta ctcgccgcgg
       61 aacccgcgag acgtcaggcc tttctgctga gtcgaccaga tggcaacgtc ctgatcgatt
      121 ccgggcccgc aggacacctc gccgaccttg ccgcgctcat gggcggcggg ggtctcgacg
      181 gacacccagt cgcgcatgga gttggagtag tactccttgg cgccctccgg aaacaaggtc
      241 aggtaccaca tgtcgaacag gcatttctcc ggatcggtgg ggtgtgggtt gccgcgcagg
      301 aagatgcacc cgtcgggctt catgctgaac gagaggttcg gaaacagcgt gtagtggtag
      361 ttgtccgtga gctgctcgtc cgaatatcgc gagaagtcgt agcccttctt ctgggccagc
      421 ttgcgcttct gctgctgcag cgcggtccgc aaaccggcga ggtcgtctcg gaagggttcc
      481 gggtcgatct cccagaacgc caggtcctga ccgaggcgct cgaacacggc ctccggggag
      541 ccctgatagt tgctgttggg cccgccaccg ggcatgaaca tgcggcagtg accgctgggg
      601 tagagatcga actggcagct cgagtagtgc tcgtccaggc tgatacacga ttgcgggtgc
      661 acgtatggca ggtgatagct ctcgttgaaa ttgtcctgga cgcacttcca gttgaagttg
      721 ccctcgaggg tcacccagtg ggttcggcgc atgtcttcca tacgatagtt gtcgagctgt
      781 cgggcaaccg ggtcgagaaa atcggcgaga ggtttgcagt cggggtccat gttgtaccag
      841 atgaaccccg cccacgtagc gcacgggatc tcgatcagat tgcgcttgcc gcaggggttg
      901 ccctggggga agtcctcctc gcagtagacc catgtgagga caccctgagc atcgaagcgc
      961 cagccatggt aggcgcactg gaactcgccg cctgacaggg atccctgctc cgcctgaacc
     1021 agctgattgc cgcgatgctg gcagacgttg tagaacgcgc ggatgctgcc gtcgtcgccg
     1081 cgcacgaaca ggatggattc gcggccgatg tccgtggtga agaagtcgcc cggctccggg
     1141 atctgatgga ccaggccgcc gatcagccag acgcgcgtcc acacgtgctc ccactcgagc
     1201 tcggcgaact ccctggaccg gtaacggtcg ccgttgatcg ggttgctccc aagctcgggc
     1261 tccgtggcct tggtccgatc aattcctcgc atctgcctta tgctgctcat ggcatgctcc
     1321 tgtctggttg accgcacggc gggtgcgcac ggacggctta ttctgcccgc ttgggggtgg
     1381 cattcaagcc cggtgcgcgg gccggcgaac gctcagctcc agatctcgaa aaactcctcg
     1441 aacgccttga gctggttgcc cacggcggac gagggcacga tgatgggcgt gcggacgccg
     1501 gcgtcgaacc aggcctcgat gccgtcgcgg accgcgttcg cgggaccgaa caaagtggtc
     1561 tcggcgagcc acgcatcgga caggcaatcg gggatcctgt cgggctcccc tgcctcgagc
     1621 gccttctcca cggcacccat ctcctccacg aagccggctt ctttccagta gttgcggtag
     1681 ttcgggagca tggcgtaacc cgtcagggtc cggcggttga cggcttttgc cgcctccacg
     1741 tcgtcgctga tgcaggtggg gatcatgttg ccgatgaaga aaccggggtc ggcgcgccgc
     1801 tcgtcaccga gcacgttcag cgagtgctgc atatggccgc gcgcggcgtt ggcgaacacc
     1861 atgccctcgg cgacctccgc ggcaagcccg atcatcctgt cgcgcagcgt ggccagcacc
     1921 acgggcggca gctcacctac ccgcggcgcg gcgcgcaggt cgctcacgaa ctggcgcatg
     1981 tccgacaggg gcttgccctg ccggacgccc agacgctcca tggccggtgc gtggctgacc
     2041 cccagcccga agcggaaccg gccattggag atctcgtgaa tgaatgccgc ggtggaagcg
     2101 aagtcgttcg cgtggcgggt gtagatcggc gtgatgctgg tcccgaactc gatctgtcgg
     2161 gtgttgtagg caatggcctc gcacagcgcc aggccgtctc ccatgctcgg gcagtagatg
     2221 cccgcaaaac cgcggcgctc gatctctgcg gcgagctcga cgatggtgtt acggcggccc
     2281 gggaccgcgg cgaggcttac tgcgggtcgt cgatcgctca tgctgagttc tccctgggaa
     2341 gtatgcggaa agcgacacag agtggcggaa cctcgtgcgc ttcgcaaccg cgtttgccgc
     2401 catgttttcg gctgcggcgg ccgcgcgtct cagaaccagc ggtgtcgcga ggagcgcctc
     2461 tcgggctggg cgagatgctg tgcgaggtga ttcggccggc ggaacaggtg tatcagagcc
     2521 accccggcca gaaacccgcc gatgtgagcc cagaaggcga cgcccccctg gctcgagccc
     2581 attgccggca aaccctgaag cagctgcagg agaaaccagt agcccagcat gaaaaccgcg
     2641 ggaacggcca cggtggtcac gtagaagccg agaaagatca gcgtgtgcac gtgggcgcgt
     2701 gggaacatga gggcgtaggc acccatgacg ccgccgatgg cgcccgatgc gccgaccatc
     2761 ggcaccaggc tggacgtgtt cgtggcgatc tgggcgaagg cggcggccag gccgcagacg
     2821 acgtagaaga cgacgaaccg gaacggtccc atgacgtcct ccacgttgtc tccgaaaacc
     2881 cacagaaacc acatgttccc gatgatgtga aaccagccgc catgcatgaa catggacgtg
     2941 aagagggagg caggattggc ggtcccgtca agctggcaga cgaggttgct gcccagcgga
     3001 atgctcgtgc cgggaacggc gttgcccagc aggtcgcccg gtatcagccc gtacagacac
     3061 agggactcgg ccagcgcgta gtggttgcca aatccctgga cggcgaccca gacgaggacg
     3121 tttagaatga tgatggcaac ggtggcgtac ggcgggtgaa cccggggatt ttcgtcgcgc
     3181 agtggaaaca tgatcggctc gtatggacct caggcacggt gttggctcgg cgcgtcggca
     3241 gatgggcgca tgaaaatcta gggtgccggg atgtcggcgc cgccgcaaga ccgggcatgg
     3301 tcttgaaggc tcagaacgtc aggctgatct tgtccgccgt gggcaggtcg cccggctcga
     3361 gcgcctcgcg cttggcggcc ttgaacgctt cgatatcgcc aacgaccatc tgccagaccg
     3421 ggtgcatgtc ccgcccgtcc accggcatgg gaaaggggct gaagccaagg ggcacgctgg
     3481 cgaacggctc gtcttggcgt cggtagagag tctgctcgga aaaatccaga tacctgggca
     3541 gaccatcgcg cgagaccatg tcgaagaaag cgacggcacc ccgaccggcc aggtccagac
     3601 gccggatcat ctctgcggga aacaggccgc ccgtccagcg ggcgttgatc tcggtgacca
     3661 ggagctcatc ggcactgacg aagtagtcga tcccgcagtt gaatccttcc ggggagcggt
     3721 agccgaggtc tctcacgtac tccccgacgc gaaggagcgc gcgctcgtgg gcggggtcca
     3781 cggtcacgcc gccgccgatc agatttccca cccagagccc gtcggcgaac aggatgcgcc
     3841 ggacgttggc gatctctatg ctgtcgtccc gcacgatcag gtccacggtc atctcggtga
     3901 actcgtccgc gctcagcttc cgttgcagca cgatctccat gtcgttcggg tactcggcga
     3961 cgtagtccat gcactccggc acgccagcca cgctggtgac gttgcgcgcg ccggccagac
     4021 ccttcagctt cagcatgatc tggttgtggt gggtgtcgaa aaagccggat acctcggggc
     4081 cgtcgaggtc gcgcttccgg cagaccaggg tttcgggcac cggtatgccc cattgcgcag
     4141 cgtccgccgc cagctgcatc ttcgagttca cgcgttggct gacggaaagc gcggcgtcgc
     4201 tgcggtgcac cacggcgttg gatccgctca ccaggtagag ggtggtcaga tccgcgtcga
     4261 cccggtccgc cgccaccgcc tcgtagaacc cgtcccgtgg atagtagtga cagttggact
     4321 cgggcacggg cggcacgccc atctcggtga acgcgcgacg atacaagtcc agcgtctcgc
     4381 gccacggcgg tcgctgaaac tcgtcgaaca aggcgacgtg atccacgggc ttgccgacgt
     4441 agagcagagc cacctgaagc atggccgtga gctctatgtt cgatacgtga tcggccagct
     4501 gcttctcggt cagcgtgttc aggtcgaagt gcaaagagca gtcgtcgccg gccatggcgt
     4561 agagcgtgga tgccgcctgg ctggggaaat acgaacccgc ctttggtgtc atgttctgac
     4621 tctccgtcgg ggttgcgatg tgttggccga agctgcggac gatagcgggc gcgcagcggt
     4681 gattgcgaag ccgtgcatac taatccctac tctccgcggc tcaggatgtc ccccacccag
     4741 gtcagcaggg tctggttctg ctcgggcgtg cccacggtga tgcgcagggt gtcccgcaag
     4801 ctgggggtgt cgaagtaccg caccagaact ccccgggtct tgagcgcctc gtagagctgc
     4861 tttgcgctgc acggagacgc cgcgggcacc tcggtcagca gaaaattcgt ctgcgaagcg
     4921 ccaacgcgga aaccgtgttc gagcaggccc tcgcgaagcg tgcggcggcc ggcgcgtacg
     4981 tttgcccagg tctcttcggc ataggccgga tcggacagcg cggcctcgcc gacccgctgg
     5041 ctcacgtagt caacgttgta gctgtcgcgc gttttgccga tgacggggtc gatcagcgag
     5101 gcgcagccga cgaggtagcc gagacgcatt ccggccaggc tgtacccctt gctgaacgtg
     5161 cgcaggatga gcagattgtc gaacgcctcg gtgaggcgca ccgcatcgta gcgaagggcc
     5221 gggtcgacga aatcgacata agcctcgtcg atcagcagaa cgccgttgaa atcattggcc
     5281 agctggctga tccgcgacgt gtcgagcagg gagccggatg gggcatgggg attgaccagg
     5341 caggcgagtc ccgcccccgc gtcgttgagc tggtgggcga agtccctcgg caggtcccag
     5401 ttctcgtcca gcgcgatgct caggacccgg gtgtcctgaa tggccgccag aacgggatag
     5461 agcgagtagc tgggatcggc cataccgaag gtgccgcccg gctccaggaa cgtggtgaag
     5521 gccagcctca agccttcgtc gccgccgttg gtgaccacga tgttctcgcg cttcaggttg
     5581 tgcagctcgg cgatctgatc gcgcaggcga tcggccgtgg gctgggggta ggcgcgcagg
     5641 gcctcgccgc tgagatcggc gagcgcccgg tccacggccg ggctcggcgg gtaggggttc
     5701 tcgttggtgt tgagcttgat gacgtcggct cgttccggct gctcgcccca ggtgtagccg
     5761 tgcatggcgc gtatgttttg acgttcgtgg gtcatgtctg gtcctcggcc ggcgtgggat
     5821 ctgcggcccg ttcggcaggt ccgagcagca ccgccagcca ctggctcgac gggttcgctt
     5881 ccatggcgat cttggcggtc atcgtcagcg gcaccgacag cagcataccc acgggtccga
     5941 gcatccagcc ccagaacacc agggagacga acaccaccag cgtggaaagt cccagccccc
     6001 ggcccatgta gcggggctcg accacgttcc ccatcagcag gttgatgacg aagaacccgg
     6061 ccccggccac cgcggccgtc agcgggccga gctggatcag cgcgagcagc accgcgggga
     6121 tcgccgcgat cagcgagccg atggtgggca cgtagttcag gagaaaggcg agcaggcccc
     6181 aaagcgcggg gaagtccacg ccgagcacgg ccagcatcag cccgacgaga acgccggtga
     6241 tgatgctgat ggtggtcttg atgccgatgt agcgattgac gttttccgcg aagcgaacga
     6301 agtggggcag gtcgctgtcc ggatcgtcta gcacctcctg aagcttgcgg ggaaagctcg
     6361 aggcctcggc caggatgaag atgacggtga gcaggatcag gaaaccgttg ctgagcgcgc
     6421 ctcccagccc gcgcagggta ttgccggcca tgaccagggc ggtgcccgga tcgaagtagg
     6481 acagcagggc ctcctcggag atgttcaccc ccgccatctc cacgtaggtc accgcgttct
     6541 cgacgagctc cgtgagccgg tcctggtaga agggcaaccg gttcgtgaaa gcgcccgccg
     6601 actgggccac caccgcgccg accccggtga tcatcacgat gatgccgagc atcacgagag
     6661 cgatggcgat tcccgccggg acccgacggc gctcgagcca gaaaacgggg gtagccgcga
     6721 tggtggcgat gaaggccgcc agcaggaaag gcaccatcag cgcctctgcc gccttgatgc
     6781 ctgcagcgat gacgatgagg gttgccacga cgatgagcgt cttgctcgtt gaacccaatg
     6841 cagccgtccg tgagggcctg tgggtcattc taggagacgt ctgtgtgtga ggctaggcag
     6901 ctcctccctg acccgccgca ccctttcggg gtctatgtct gccaccgcca cgccatcgcc
     6961 gtcccggcat tcggcaacga tgtcgcccca cggatcgacg atcagggagt gaccgtagga
     7021 gaagcgtttg ccccagttgt ggccgtgctg tgccggggcg atgacgtagc tctgggactc
     7081 gatggcccgc gcccgcagca gcacgtgcca gtgagcggcg ccggtggtct tggtgaacgc
     7141 cgatggaacc gtcatcgcaa cggcgcccgc gtccaccagg gactgataaa gataggggaa
     7201 ccggaggtcg tagcagatgc tcaagcccag cttgccgaag ggcatgtccg tggtgaccag
     7261 gtcatggccg gccagcgtgc gggccgactc gcgcagctgg gtgccgtctg ccagcgccac
     7321 gtcgaacagg tgcacttttc ggtatcgagc cagcaactcg ccgccgggag aaaggtgaac
     7381 acaggtgttg tagctcttgt cgggatcgcc tgcatcctcg tgaaagccgc cgagtatcag
     7441 gtggcagccc aggcgcgcgg ccattgaccg gcactgttcc aggatcggac cgcccggcgc
     7501 gctcggggcg tcggggagcc gctccaggat gccgcgcttt gccttttccg gtccgagaaa
     7561 tgcgaaggcc tcgggcagca gcacgacctc ggcgcccgca tcgcaggcct ggcggacgag
     7621 acgctcgacg gcaatcatgt tggccgtgac gtccggcgtc gcgcatagct ggacgacgcc
     7681 taccctggtt agacggtggc cttggttcac ggcaccgggg tcccctgcgg cttgggcggt
     7741 accgttgccc gctcagaagc tcggctgggt gcagaccacg gcctcaccaa gatattcctc
     7801 gatgctgggc aggttgaacg cgtcgtcttc cgagacgaag ctgatgctca caccctctgc
     7861 cccggcgcga cctgtacggc cgatgcgatg cacgtagtcc tcgggatctt ccgggaggtc
     7921 gaagttgatg acgtggctta tcgcctcgac gtggatgccc ctgccggcca cgtccgttgc
     7981 cacgacgtag gggatgtcgc cgtccttgaa ccgcgacagc gtcgaaaggc gttttctctg
     8041 gggcacgtcg cctgccaggg cctcgcagcg gatattgcgc ttgttgagga acgcgtggag
     8101 ctggtgggtc tgatcacggc ggttcgtgaa tatcagagcc cgctccggcg catgcctggt
     8161 gaggaactcc gcgagcagcg aagccttttt gtcggcggcg accagccaga acttctgggt
     8221 gaccgtatcc gtcgccacgg attccggggt gatggcgacg tgctctggat cgatggtcca
     8281 ggaattggcc agccgcatca cgtcgtcatt gaaggttgca ctgaagaaca gcgtctgccg
     8341 gttccgtttg tggggcgtct gatagacgat gcggcggacg tcgggaacga agcccatgtc
     8401 gagcattcgg tcggcctcgt cgatgaccag gacctccacg tcccgcaggc tcaggtgacg
     8461 gcggctgatg aagtcgagca gccggccggg ggtggcgatc agcacgtcga cgggacgccg
     8521 cttgatgcgg tccagctgct tctgaaagtc catgccgccg accacgcact ccacaccgag
     8581 gtcgatgtac ttggtcaggt ccttggcatc gctttcgatc tgcagagcga gttcccgggt
     8641 cggtgccagc accagcgaac gaggcgaacc gatcttgggc gccgcggcgg gagggtactc
     8701 ccacaacctg gcaatgatcg tgaccaggaa cgccgccgtc tttccggttc cggtctgggc
     8761 ctggccggtg acgt
//
LOCUS       DBNHKV010000286         4461 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_112951, whole genome shotgun sequence.
ACCESSION   DBNHKV010000286 DBNHKV010000000
VERSION     DBNHKV010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_112951"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="AC16PP_09045"
     CDS             <1..235
                     /locus_tag="AC16PP_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481814.1"
                     /translation="ENEHLHPAAHLMRNIHTRENETGVELRFRGILPRNQIQADGSAT
                     ILHGSYYDQVVKTPAGWRVQTRSFLLERRDRQK"
     gene            315..1328
                     /locus_tag="AC16PP_09050"
     CDS             315..1328
                     /locus_tag="AC16PP_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481815.1"
                     /translation="MTHVFANAPTELRDLIAATSQSEFATLTKQLVPIANPLFHYYDS
                     GDPTIDIATGLAYPAKADRVRSNPKVGLLFGPAVHAHDPIAILEAGAPDSRNLADQPV
                     VAIAAIGAVRDADLQANTDKYVDSFLAEHPNIGPTDWDTLKTMTNYWVRIWVECAPCR
                     VYWWPTGKLNDEAPMIWEAPPGMDFPSSDPAPAKKGTPRARWPAEPWVERAEAVLHQF
                     PRPILTSMTEDGFPLPMPTNSARMNQEGFELDIPVHGPVRAEEGNACLSFGALATFTG
                     HLSPGVQGTNFVVDRLIGNLPSIFQNEGEQFDAMAKRQVAELERRGQPMPEIRKGYSP
                     RGR"
     gene            1708..2286
                     /locus_tag="AC16PP_09055"
     CDS             1708..2286
                     /locus_tag="AC16PP_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6481816.1"
                     /translation="MSKATDHEVVSIYRHSDEQIDELMTRAPECVLMWGTQDHWPVGV
                     VHSFVWRDGKVWLTFTAHRHRATAIRRDPRVSVCVSGRTSDDPDCPLGAVTLKGRAVF
                     HDDDATKDWFYTALAQKVAQGDKAVEDDFRNLLDSPLRVILEITPEKWISFDAAKSAR
                     HRAGLLSDDEMSPLQSADAERLKKEREQRGID"
     gene            2564..3514
                     /locus_tag="AC16PP_09060"
     CDS             2564..3514
                     /locus_tag="AC16PP_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6481817.1"
                     /translation="MEYEFGVTSVGPVLRRLSFDSVDRAEAELQLCGVDQHMRQIGSR
                     SFQAKISSAYVNGLELISERYEAAISLSCGPPENSIAIIFAGTAEGQSRVNGSDVSDG
                     GLVFLPLASDLDCVFHGRFGSESIIIPEDRFNSIVSSVCPESPFPKTATIVKINQART
                     AGLRAEIAALLNQSMPNAERVTDLVDATITYVSELQFRVASSRPDSGSYRRIAKQAQE
                     FIEARFMGPIRIEDICRSCGVGVRTLQRAFRDYFGLTVTEYVKTTRLDKAFRALKAAV
                     PAEATVADVAANNGFTHLGRFSVLYKERFRESPNSTLRSF"
     gene            3640..>4461
                     /locus_tag="AC16PP_09065"
     CDS             3640..>4461
                     /locus_tag="AC16PP_09065"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR6481818.1"
                     /translation="MQRHTKCTMYRRNMPAICYLLLLGALCLAPPLSAQGKPNIVLVF
                     LDNFGWGEPGFNGGGIIRGAATPRLDQIAAEGLRLTNFNVEVQCTPSRSALMTGRYAI
                     RSGNGTVPLGEGVYGLVQWEVTLAEMLSEAGYATGMYGKWHLGRTEGRFPTDQGFDEW
                     YGIPNSTDESVYSSVQGFADSGIAESFVMESKRGSPPKKVRPYRLDYRPLIDRDLTDR
                     AISFMRRQARAGNPFFVYLPYTATHFPTMPHPDFVDRSGKGPWGDLLMQIDSYTGE"
BASE COUNT          984 a         1279 c         1301 g          897 t
ORIGIN      
        1 tgagaacgaa cacctgcatc ccgccgcgca tctgatgagg aatattcata cccgagaaaa
       61 cgaaaccggt gtggaattac gctttcgagg catcctgccc cgcaatcaga tacaggctga
      121 tgggtcggcg accattttgc acggttccta ctacgaccag gtcgtgaaaa cgccggcggg
      181 ctggcgggtg cagacacggt ccttccttct cgagaggcgc gataggcaga agtagctcac
      241 gaaggaacga gtcttcctga acggtgcggc ggcgtatgtt tgtccgcgac gagtgaaaga
      301 cgaggtttat cgaaatgacc catgtatttg ccaatgcgcc caccgagctg agggacctga
      361 tagcggcgac gagccaatcc gaatttgcga cgctcacgaa gcagctcgta ccgatcgcca
      421 atccgttgtt ccactactat gattcgggtg atccgacgat tgacatagca accggtctgg
      481 catacccggc aaaggccgat cgcgtccgaa gtaacccaaa ggtcggcttg ttgttcgggc
      541 cagcagtaca cgctcacgat ccgatcgcga tcttggaagc aggcgcaccg gatagccgaa
      601 acctcgcaga ccagccagtc gtggccattg cagcgatcgg cgctgtcagg gatgcggatc
      661 tgcaggcgaa cacagataag tacgtagaca gtttcctggc cgagcatccc aacattggtc
      721 ccactgactg ggacacccta aagaccatga ccaactactg ggttcgcata tgggtggaat
      781 gtgctccatg tcgagtttat tggtggccaa caggaaaact caacgatgag gcgccaatga
      841 tctgggaggc accgcctgga atggattttc cgtcttctga tcccgctccc gcgaaaaagg
      901 gcactcctcg tgcgcgttgg cccgccgagc catgggttga gagagcagaa gccgtgcttc
      961 accagtttcc gcggccaatt cttacctcca tgaccgagga cggtttccca ctgccgatgc
     1021 caaccaacag cgcccggatg aatcaggaag gcttcgaact cgatattccc gtgcacggcc
     1081 cagtccgcgc agaggaggga aacgcttgcc tctctttcgg ggcgctggct acgtttacgg
     1141 gtcatctgtc gcctggcgtc caggggacaa acttcgttgt cgatcgcttg atcggtaacc
     1201 tgccaagcat ttttcaaaac gagggagagc aattcgatgc aatggcaaag cgacaggttg
     1261 ccgagctcga acgccgtggc cagccgatgc cggagatcag aaaaggctac tccccacgtg
     1321 gtcgatagca gcttcaagac gcagtgaacg atatggctcc gcctgctggg ctcgactggc
     1381 cgtcacgcgg caggcgcccg ttgcagccag atgcctcagg atgcacaacc gtgcactcct
     1441 gatccccgca aaaccaccac aggcgccgcg atatcggaca tccgtagctg acgacctgcc
     1501 aatcccacga cacgttcgag agcattcgct tgccacgtcg gatgcagttc agcagctcct
     1561 ccagcgaatg ccgcctggtt tgcgggcggc gttttggcgg cggggattcc ccacagcgca
     1621 ttcctaccag ccgtgccgga acggccacgg tgacttcgtc gcgcgacgca tgctaacgtc
     1681 gccggattca cgatgcggga gagacagatg tccaaagcga cagaccacga agtcgtttcc
     1741 atttaccgcc acagcgacga gcagatagat gagctcatga cgcgcgcgcc agaatgcgtg
     1801 ctgatgtggg gcacccagga tcactggccg gttggcgtcg tccactcgtt cgtctggcgc
     1861 gacggcaagg tatggctcac ctttactgcc caccggcatc gggccaccgc aattcgacgc
     1921 gatccgcgag tttcggtgtg tgtgagcggg cgaaccagcg acgatcccga ctgtccgctg
     1981 ggggcggtaa cgctgaaagg gcgtgcggtt tttcacgacg acgatgccac caaggactgg
     2041 ttctacactg cgctggcaca aaaggttgcc cagggcgaca aggccgtgga agacgacttc
     2101 cgcaatctgc tggactcgcc gctgcgggta atcctggaga tcactccgga aaaatggatc
     2161 tctttcgatg cggccaagtc tgcgcggcac cgggccgggt tgctgtcgga cgacgagatg
     2221 tcgccgctgc agagcgccga cgcggaacgg ctgaaaaagg agcgcgagca gcgcggtatc
     2281 gactaagcat ctgagaaaag gatagaagca tacgggcgcg acaggggtcc aggtcgagta
     2341 tcaggaggta cgactccttc cagacccccg aaacatcccc gcaagaggga ccttgagatg
     2401 agcgaagaag gtgctaagtc gttgaaagat gatgcatgac cgatctccac aaaattcccg
     2461 caagcaacgc gacatcggat gtctgctcta taacgtcaga ccaccgtttt ttgcagtgca
     2521 tctgaagtac tatgattcgc tcatcagggt cgattaagca ttcatggagt atgaattcgg
     2581 agtaacttcc gtaggccccg tgcttcggcg gttgtccttt gattctgtcg accgtgccga
     2641 agcggaacta cagctttgcg gcgtcgacca gcacatgagg caaataggat cacggtcatt
     2701 tcaggcgaaa atctcatcgg cctatgtgaa cgggttagag ctcatctcag agcgctatga
     2761 agcggccata tctctgagct gcggcccgcc agagaattcc atcgcgatta ttttcgccgg
     2821 cactgccgag ggacagagtc gcgtgaacgg tagcgacgta agtgatggcg gattggtttt
     2881 tcttcctctg gcaagcgatt tggattgcgt cttccacggc cgttttggtt cggaatccat
     2941 catcatcccg gaggatcgtt tcaacagcat cgtttcctcc gtctgtccgg agagtccctt
     3001 tccgaaaacg gcgactatcg tgaagatcaa tcaggcccgg acagcaggcc tgcgagctga
     3061 gattgccgcg ctgctcaacc agagcatgcc gaacgctgaa cgcgtcaccg atctggtcga
     3121 tgcgaccatc acctacgtga gcgagttgca gtttagggta gcttcttcgc gaccagattc
     3181 cgggtcctac cgccgcatcg ccaagcaggc acaggaattc atcgaagctc gctttatggg
     3241 tccgattcgc atcgaggaca tctgtcgatc ctgcggcgtc ggagtgcgga cactgcaacg
     3301 tgcgtttcga gactatttcg gcctaaccgt aaccgagtac gtaaagacca ctcgtctgga
     3361 caaggcattt cgggccctga aggcagccgt tccggctgaa gccacggtag ctgacgtcgc
     3421 ggcaaataat ggtttcacgc acctaggtcg cttttctgta ttgtataagg agagatttag
     3481 ggagtcgccg aacagcacgc tgcgttcgtt ctgatcgacc gaaccccacc gatctcggat
     3541 tccaccggca cttggcttgc actgatgacg caaagtggat agacagcatc acagctgtta
     3601 cgggatgctg atccctgatc taactgggaa agcgtgaacg tgcagcgaca cacgaaatgc
     3661 accatgtatc ggcgcaacat gcccgcgatc tgctacctcc ttctgctcgg ggcgctgtgt
     3721 ctggcgccgc ccttgtctgc gcagggaaag cccaacatcg tccttgtctt tctggacaat
     3781 ttcggctggg gtgagcccgg attcaacggt ggcggcatca ttcggggagc cgctaccccg
     3841 aggctggatc agattgccgc tgaaggcctt cgtctgacga atttcaatgt cgaggttcaa
     3901 tgcacgccgt cacgatccgc actcatgacg ggacgctacg cgatacgcag cggaaacggc
     3961 actgtgcctt tgggagaggg cgtatacggt ctggtgcagt gggaggtcac cctggccgaa
     4021 atgctttcag aagcgggcta cgcgaccgga atgtacggca aatggcacct cggacgcacc
     4081 gaaggacgct tccccaccga tcaggggttc gatgagtggt acggcatacc caactccacc
     4141 gatgaatcgg tttattcctc cgtgcagggt tttgccgaca gcggcatcgc agaatcgttc
     4201 gtgatggaaa gcaagagagg gagtccaccg aagaaggttc gtccctaccg tctggactat
     4261 cggccgctga tagatcgcga tctgacagac cgggcaataa gcttcatgag gcggcaagcg
     4321 cgggccggta atccattctt cgtttatctg ccatataccg ccacccactt tccgacaatg
     4381 cctcacccgg actttgtgga ccgttccggc aaaggtccct ggggtgatct cctgatgcag
     4441 atcgacagct acacgggaga g
//
LOCUS       DBNHKV010000287         5772 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_821139, whole genome shotgun sequence.
ACCESSION   DBNHKV010000287 DBNHKV010000000
VERSION     DBNHKV010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_821139"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="AC16PP_09070"
     CDS             <1..267
                     /locus_tag="AC16PP_09070"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR6481819.1"
                     /translation="EIGRPRDNLTDMETNIRYGTTILAHYIEVSDGDLVDALARYNGS
                     RGRLKYPEKVVGAWRRVWRHKSSDELPDLVSTCATYALHACRYR"
     gene            complement(314..805)
                     /locus_tag="AC16PP_09075"
     CDS             complement(314..805)
                     /locus_tag="AC16PP_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481820.1"
                     /translation="MLRKLAIRDVLIVAVATAGWLGLLPLSEGRPLISDSLALLLGLG
                     VTVSAYLLHEWGHALAGLVVGSRIHAPASLRSVSLFSFDAEANSKTQFAAMSIGGFVV
                     TAAAMGFVYFVLPDGYMASRVARGGVTVLASITVFIEVPLLLYGLLARRLPGVVAVFR
                     PET"
     gene            complement(808..1287)
                     /locus_tag="AC16PP_09080"
     CDS             complement(808..1287)
                     /locus_tag="AC16PP_09080"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIR6481821.1"
                     /translation="MKAKTSSLLLAGALFLAGCDFLGQADEPPLALADGTTMKVDDWH
                     GRWLLINYWAEWCAPCRKEIPELNRLQAEVGEERLLVIGVNFDGLTGQRLTELIEKMD
                     VRFPVLVEDPRGRWDVEQPTVLPSTLVIAPNGRLKEVLVGPHTFESFSRALGLTAST"
     gene            complement(1284..2513)
                     /locus_tag="AC16PP_09085"
     CDS             complement(1284..2513)
                     /locus_tag="AC16PP_09085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HIR6481822.1"
                     /translation="MLEATYDQFQERVVVPLRWYVGQLGQQFLIHDCMTRAGALTYTT
                     LFAVVPMMTVAYAMFSVLPEFETVGERIQEYIFQNFVPDSSAVVQERLLEFSARARQL
                     TAAGFLVLFITAFMMLVSVEKTFNTIWSVAEPRRGLQRFLLYWGVLSLGPPLIAAGIF
                     ISLYLISLPLIADLDTFGLDSAVLDYLPSVLTAAGLTVLFLAVPNCRVPFHHALAGGV
                     LTMFAFELAKKVFRLTVANSSMEPIYGTFAAVPLFLIWLYLVWVLILSGAIFVRTLSL
                     KRDRPVDNPEPAVVKCSRVVKFLHAAHLEGRSVSDSEINDQVPLARAERERVFEALLA
                     LKLVRETEDGGWMLGRSLKTLTLWDLYRALPDGVDAARLGTVKDMDGVVEPLKAFVQF
                     GSNQMAVSLDSVFGKSS"
     gene            2576..2926
                     /gene="arsC"
                     /locus_tag="AC16PP_09090"
     CDS             2576..2926
                     /gene="arsC"
                     /locus_tag="AC16PP_09090"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252402.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HIR6481823.1"
                     /translation="MREVTIYHNPRCSKSRATLALLEEHGVTPNVVEYLSTPPDAATL
                     KRLLDLLGLSPRELMRRKETVYSDLGLDDDALGEDALIEAMIANPILIERPIVVAGDR
                     AVIGRPPENVLGLV"
     gene            2930..3538
                     /gene="wrbA"
                     /locus_tag="AC16PP_09095"
     CDS             2930..3538
                     /gene="wrbA"
                     /locus_tag="AC16PP_09095"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HIR6481824.1"
                     /translation="MTGPVHPYVLILYYSRTGGTAHLARYVARGVQAQGDFEARVRTV
                     PPVSTTVEQTEPVVPEHGAVYCTKADLAGCGALAIGSPTRFGNMAAPLKHFLDDTADL
                     WMQHALVGKPAGVFCSTASLHGGQESTLLSMMIPLLHHGMLVSGVPYSEPALNRTARG
                     GGPYGPSHVSGGEAGTEPTADEQAIAMALGRRLAELARRLGA"
     gene            3535..3885
                     /locus_tag="AC16PP_09100"
     CDS             3535..3885
                     /locus_tag="AC16PP_09100"
                     /inference="COORDINATES: protein motif:HMM:NF021369.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HIR6481825.1"
                     /translation="MKGLQLACLLMMGSLFAINGLRQFFVEPLATPHVNLIWFLIQVL
                     PLLAVLPGTLRNRYRSYVFAALVSTLYFIHGVLLAATADLRALGLWETGFAVGLLIAA
                     SYAARRTVGQAASQ"
     gene            complement(3879..4214)
                     /locus_tag="AC16PP_09105"
     CDS             complement(3879..4214)
                     /locus_tag="AC16PP_09105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6481826.1"
                     /translation="MIIVHGTIPIRPDQRERALELARRMSEATQAELGCISYDFYVGL
                     SDPNTLMLFQEWETMEALMAHFQTEHMDDFLRELPQVVAGEITTRRYAVQSVDEDTEE
                     TPETPPIVH"
     gene            complement(4246..>5772)
                     /locus_tag="AC16PP_09110"
     CDS             complement(4246..>5772)
                     /locus_tag="AC16PP_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016562180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6481827.1"
                     /translation="EAAIAMLACARIGAIHSVVFGGFAAPELATRIDDAEPKVVLSAS
                     CGIEFANVIPYQPLVDEAISLAVHKPERVVMLQREQQEADLSGERYLDWASWEASAQG
                     VGCVPLDATDPLYVLYTSGTTGKPKGVVRDNGGHAVALNYSMEAIYGMDAGDVFWAAS
                     DVGWVVGHSYIVYGPLIRGCTSVLFEGKPVRTPDAGVFWRVIQDHRVKCFFVAPTAFR
                     AIRKEDPDCALKDRYDVSSLKYQFVAGERLDPPTYHWLSEHLHVPVIDHWWQTETGWA
                     MCANMAGLELKDAKPGSPTLPVPGYDLRILDEDGVEVPAGVEGAVVVREPLPPGAFPT
                     VWGDHQRYVDSYWSRYPGFYLTGDEGYRDDEGYVYIMGRMDDVINVAGHRLSTGQMEE
                     TVAEHPAVAECAVVGIADADKGQVPVGLVLVKDGVNVDPQALEAELVQMVRASVGAFA
                     NFRRAVVVERLPKTRSGKILRQVLRKMIDGNPYSVPSTIDDPAIIPEMASTLKKAGVM
                     "
BASE COUNT         1104 a         1952 c         1789 g          927 t
ORIGIN      
        1 gagatcggcc gaccccgtga caacctcacc gacatggaaa ccaacatccg ctacggcacg
       61 accattctgg cgcactacat cgaggtatcg gacggcgacc tggtagacgc tctggcacgc
      121 tacaacggca gccgcggccg cctgaagtat ccggaaaagg tcgtcggcgc ctggcgccgc
      181 gtctggcgcc acaaatcgtc cgacgagctg cccgacctcg tatccacctg cgcaacctac
      241 gccctgcacg cctgccgcta ccgatagggc atcgtctcga tctcgacggt tccggcaaac
      301 aacggtccag cggtcaggtt tccgggcgga ataccgccac gacgcctggc aggcgcctgg
      361 cgagcaatcc gtacaaaagc aggggcacct cgatgaaaac ggtgatcgag gccagcacgg
      421 tcaccccgcc ccgagcaaca cgggaagcca tatagccgtc gggcagcaca aaatagacaa
      481 agcccatcgc ggccgccgtg accacgaacc caccgatgga catcgcggcg aactgggttt
      541 tgctgttggc ttcggcgtcg aagctgaaca gcgacacgct gcgcagcgag gccggcgcgt
      601 ggatgcggct gccgaccacg agaccggcga gcgcgtgacc ccattcgtgc aggaggtagg
      661 cactcaccgt cacgccgaga ccgagcaaca gggccagcga atcgctgatg agcgggcgtc
      721 cttcggacaa tggcagcagg ccgagccagc cggcggtcgc caccgccacg atcagcacgt
      781 cccggatggc gagcttgcgc aacatcgtca cgtggacgcg gttagaccca gggcccggct
      841 gaagctctcg aaggtatgcg gccctaccag cacctccttg aggcgaccgt tgggcgcgat
      901 gaccagcgtg ctcgggagca cggtgggctg ctccacgtcc catctgcccc gagggtcctc
      961 tacaagcacc ggaaaccgga cgtccatctt ttcgatgagc tccgtgagcc gctgcccggt
     1021 gaggccgtcg aagttgacgc cgatcaccag tagccgctcc tcgcccacct ccgcctgcag
     1081 gcgattgagc tccggaatct ccttgcggca cggcgcgcac cactccgccc agtagttgat
     1141 cagcagccag cgcccgtgcc agtcgtcgac cttcatggtc gtaccgtcgg ccagagccag
     1201 aggcggctcg tctgcctggc cgagaaaatc acagccggcc aggaaaagcg caccagccag
     1261 caagagagag ctcgtcttcg ccttcatgag gatttaccaa acaccgaatc gaggctcacc
     1321 gccatctggt tggagccgaa ctggacgaag gccttgagcg gttcaaccac gccatccatg
     1381 tccttcaccg taccgagccg tgctgcgtcc acgccgtccg gaagcgcacg gtagagatcc
     1441 caaagcgtca gcgtcttcag gctgcgacca agcatccaac ccccatcctc cgtttccctc
     1501 accagcttga gggcgagcag cgcttcgaat acccgctccc gttcggcccg ggccagcggc
     1561 acctggtcgt tgatctccga gtcgctgacg cttcggccct cgagatgggc agcgtggaga
     1621 aacttcacga cccgcgaaca cttgaccacc gcgggctcgg ggttgtccac gggccggtcg
     1681 cgcttgagcg acagggtgcg gacgaaaatc gccccgctga ggatcagcac ccagacgagg
     1741 tagagccaga tcaggaaaag gggcaccgca gcaaaggttc cgtagatggg ctccatgctg
     1801 gagttggcaa ccgtgagcct gaataccttc ttcgccagct cgaaggcgaa catcgtcagg
     1861 acgccgccgg cgagggcatg gtggaaggga acccggcagt tgggaaccgc gaggaagaga
     1921 acggtcagcc ccgccgcggt cagcacgctc ggcaggtaat cgaggacggc gctgtccagc
     1981 ccgaaagtgt cgagatcggc gatcagagga agggatatga ggtacagaga aatgaaaatc
     2041 ccggcagcga tcagcggcgg gcccagggag agaacgcccc agtacagcag gaatcgctgc
     2101 agtccccgcc gcggctcggc tacgctccag atggtgttga acgtcttctc cacggagacc
     2161 agcatcatga aagccgtgat gaacagcacc agaaagcccg ccgcggtgag ctggcgcgcc
     2221 cttgccgaga attccaggag gcgttcctgg accacggcgc tgctgtccgg cacgaagttc
     2281 tgaaatatgt attcctggat ccgctcgccg acggtctcga actccggcag cactgaaaac
     2341 atggcgtacg ccaccgtcat catcggcacc acggcgaaga gcgtggtgta ggtcaacgct
     2401 cccgcccgcg tcatgcagtc gtggatcagg aactgctgac ccagctgccc gacgtaccag
     2461 cgcagcggca cgaccacccg ttcctggaac tgatcatacg tcgcctccaa cattgccagt
     2521 ctcccgcgcg accggttaaa tgaaatcgcc gccagaaccg aacattataa agaccatgcg
     2581 tgaagtcacc atctaccaca atccgcgctg ctccaagtcg cgggccacgc tggccctgct
     2641 cgaagagcac ggggtgacgc ccaacgtcgt cgagtacctg agcacgccgc ccgacgcggc
     2701 gaccctcaaa cggctgctcg acctgctcgg actctccccg cgagagctca tgcgccggaa
     2761 ggaaaccgtc tactccgatc tcggtctgga cgacgacgcc ctcggcgagg atgccctcat
     2821 cgaggcgatg atcgccaacc cgatactcat cgagcggccc atcgtcgtcg ccggcgacag
     2881 ggccgtcatc ggccggcctc ccgagaacgt cctggggctg gtctgaccca tgaccggacc
     2941 cgtgcacccc tacgtcctga tcctctacta cagccgtacc ggcggaaccg cgcacctggc
     3001 ccgatacgtc gcgcgcggcg ttcaggccca gggtgacttc gaggcgcggg tgcgcaccgt
     3061 gccgcccgta tccaccacgg tggagcagac cgagcccgtg gtgcccgagc atggcgccgt
     3121 ttactgcacg aaggcggacc tggccggatg cggcgccctg gccatcggct cccccacccg
     3181 attcggcaac atggcggcac cgctgaagca cttcctcgac gatacggccg atctgtggat
     3241 gcagcacgcc ctggtgggca aaccggctgg cgtgttctgc tcgaccgcct cgctgcacgg
     3301 cggccaggaa tccaccctgc tgtcgatgat gatcccgctg ctccaccacg gcatgttggt
     3361 ctccggcgtg ccttacagcg agcccgccct caaccggacc gccaggggcg gtgggccata
     3421 cggccccagc cacgtcagcg gtggcgaggc ggggaccgag ccaacggcgg acgagcaggc
     3481 aatcgccatg gccctggggc gcaggttggc cgagctcgcc cgtaggctcg gcgcgtgaaa
     3541 ggactgcagc tcgcgtgcct gctgatgatg ggcagcctct tcgcgatcaa cgggctgcgc
     3601 cagttcttcg tggagccgct ggccacgccc catgtgaacc tgatctggtt tctgatccag
     3661 gtgctgcccc tgctggccgt gcttccaggc acgctgagga accgctaccg aagctacgtc
     3721 ttcgccgcgc tggtgagcac cctttacttc atacacggcg tgctgctggc cgccacggcc
     3781 gacctcaggg cgctgggcct gtgggaaacg ggctttgccg tcggcctgct catcgcggcg
     3841 agctacgccg cccggcgcac ggtagggcaa gctgcgtctc agtgaacgat gggcggcgtc
     3901 tctggcgtct cctcggtgtc ttcgtcaacg ctctgcacgg catagcgccg ggtcgtgatc
     3961 tcccccgcca ccacttgcgg aagttctcgg aggaagtcgt ccatgtgctc ggtctggaag
     4021 tgagccatca gcgcttccat ggtctcccat tcctgaaaaa gcatcagcgt gttgggatcg
     4081 gacagcccca cgtagaagtc gtaggagatg cagccgagct ctgcctgggt tgcctcgctc
     4141 atccggcggg cgagctccag agcgcgttcg cgctgatccg gacggattgg gatggtgccg
     4201 tgaacgatga tcatctgcct gttgccaatg ttagcagcag cccggtcaca tcacccccgc
     4261 tttcttcaaa gtcgaggcca tctccgggat gatcgcagga tcgtcgatgg tggagggaac
     4321 gctgtaggga ttgccatcga tcatctttcg cagcacctgc cgcaggatct ttcccgagcg
     4381 cgtcttgggc agacgctcca cgaccaccgc ccgccggaag ttggcaaaag cgcccaccga
     4441 tgcccgaacc atctgcacga gctcggcttc aagcgcttgg ggatcgacgt tgacaccgtc
     4501 cttgaccagc accagaccga cgggaacctg gcctttgtcc gcatccgcaa tgccgaccac
     4561 ggcgcactcg gccaccgcgg gatgctcggc gaccgtctcc tccatctgcc cggtggagag
     4621 ccggtggccg gctacgttga tgacgtcgtc catgcggccc atgatgtaga cgtaaccttc
     4681 gtcgtcgcga tagccctcgt cgccggtcag gtagaagccg ggatagcggg accagtagga
     4741 atcgacgtag cgctgatgat cgccccaaac cgttgggaaa gcgccgggcg gcaggggctc
     4801 cctgaccacg accgcccctt ccacgccagc aggcacctcg acgccgtctt catcgagaat
     4861 gcgcaggtcg tagcccggaa cggggagggt cggcgagccc ggtttcgcgt ccttgagctc
     4921 cagaccggcc atgttcgcac acattgccca gcccgtttcc gtctgccacc agtggtcgat
     4981 cacagggacg tgcaggtgct cgctcagcca gtgataggtg ggcgggtcga ggcgctcgcc
     5041 ggcgacgaac tggtacttca gggaggagac gtcgtaacga tccttcagcg cgcagtcggg
     5101 gtcctccttg cgaatcgcgc gaaaggccgt tggcgcgacg aagaagcact tcacccggtg
     5161 atcctgaatc acgcgccaga aaacgcccgc gtcgggggtc cgcacgggct tcccttcgaa
     5221 caggaccgag gtgcagccac ggatgagggg tccgtagacg atgtaggaat ggcccaccac
     5281 ccagccgacg tccgacgcgg cccagaaaac gtcgcccgcg tccatgccgt agatggcttc
     5341 catgctgtag ttcagcgcca ccgcgtggcc gccgttgtcc cgcaccacac ccttgggctt
     5401 tccggtggtg cccgacgtgt aaaggacgta gagcggatcg gtggcatcga gcggcacgca
     5461 gccgacaccc tgggccgagg cctcccagct ggcccagtcc agataacgct cgccggaaag
     5521 atccgcttct tgctgctcgc gctgcagcat caccacgcgt tccggtttgt ggacggcaag
     5581 ggaaatggct tcgtctacga gaggttgata cgggatgacg ttggcgaact cgatcccgca
     5641 cgacgcgctc agaaccacct taggctcggc atcatcgatc cgggtagcga gctcaggcgc
     5701 ggcaaacccg ccgaaaacca cagagtgaat ggcaccgatc cgcgcacagg ccagcatcgc
     5761 aatcgccgcc tc
//
LOCUS       DBNHKV010000288         3215 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1670827, whole genome shotgun sequence.
ACCESSION   DBNHKV010000288 DBNHKV010000000
VERSION     DBNHKV010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1670827"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="AC16PP_09115"
     CDS             complement(<1..867)
                     /locus_tag="AC16PP_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR6481828.1"
                     /translation="MSKKELRLAVVIYGGASLAVYMHGVTKEMLKLVRASKVFHQMGI
                     GRARYASYAEGPDNRQADTEAVYFEILKEINGRSHFRVVLDVIAGASAGAINSVMLAK
                     AIVDDASLDSQTGTWLSDADVEYMGRETSPWQKWWLYPILRLLFFWLPRDVGASPETR
                     EKLARVVRSSWFQPPLSGARLSHIFFNSLEAMLKTRLPGSSLLPAGQRLDVYTSITDL
                     VGYPRSIRLHDELMASEREHGAYCRFTHMETEAGRRLSDLDDANLPGLVWAARASSSY
                     AGAFEPFHHSELK"
     gene            913..1602
                     /locus_tag="AC16PP_09120"
     CDS             913..1602
                     /locus_tag="AC16PP_09120"
                     /inference="COORDINATES: protein motif:HMM:NF016300.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR6481829.1"
                     /translation="MSRIQIIALLCCAMLLAAPTIGRPQVEGIHVSGRGEVLVTPDMA
                     RVTLRVTRDGQDAVELKKELDDVTRKVLSLTRSLKIEGEDVTAAVVNIQPRYRRGGGQ
                     SVIDGVTASRTIAVVLRDLDRYGQLMNGALEVGVNSVSGTQLDSSDRAAEEEQALERA
                     MQDARAAAARVADGFGVSVGRLLDVHVGQPPVQPMRGVMMEAAAAGDDFSPGQLVISR
                     QLQATFAIETR"
     gene            1697..2818
                     /locus_tag="AC16PP_09125"
     CDS             1697..2818
                     /locus_tag="AC16PP_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6481830.1"
                     /translation="MNHRNIVAAIGCLALVACGEAPIDDAAPTARPVKTMIVESPAGS
                     GVRNFPGRIDSANRADLAFRVPGTVARLAASEGAPVNRGQELARLDQTDYEIALRDRQ
                     ATWDRASKDYERSKDLVDKGAISRRDFDRVEANFKSADAALAQAKQNLEYTTLRAPFD
                     GNVARRHVDAFEEINAGQTVYSVIDQSVLEVKIDIPENVIMMLSSRGSEPEEQAGKIP
                     VWAAFDMAPERRFPLTVKEYATRADAQTQTFEVTFALPAPDDVRVLPGMTASVTMDLS
                     RAMDVDSVYYLPVTAVTGSEGLTPRVWAVDEDTMTVREQPIVLGRLVGSSVEVTEGLE
                     PGMRIVTAGAAYLADGMAVTLLAHSEQAEPREEDLPRPS"
     gene            2852..>3215
                     /locus_tag="AC16PP_09130"
     CDS             2852..>3215
                     /locus_tag="AC16PP_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6481831.1"
                     /translation="MNIGSYSVENKVISWLLVIILVAGGIYGFNEMGKLEDPNFTIKQ
                     AKIITLYPGATAQEVQDEVTYHIEEALQLMGQLKRIKMSISRPGMSDLAIEFKDEYKA
                     DDFPDIYDELRRKLQDMHG"
BASE COUNT          630 a         1037 c         1045 g          503 t
ORIGIN      
        1 cttgagctcg gagtgatgaa acggctcgaa agcccccgca taggacgagc tggcccgggc
       61 cgcccagact aggcccggca ggttggcgtc gtccaagtcg ctgagccggc ggccggcctc
      121 ggtttccatg tgggtgaatc ggcagtaggc accgtgctcc cgctcactgg ccatcagctc
      181 gtcgtgaagg cggatgctgc ggggatagcc gaccaggtcc gtgatgctcg tgtacacgtc
      241 caaacgctga ccggcgggca gcaggctgct ccctgggagc cgggtcttga gcatggcctc
      301 cagagagttg aagaagatgt ggctcaaccg ggcccccgac aacggcggct ggaaccagga
      361 ggagcgcacg acgcgggcga gcttctcgcg ggtttccggg ctggcaccga cgtccctcgg
      421 aagccagaag aacaggagcc ggagaatcgg gtaaagccac cacttctgcc agggcgacgt
      481 ctctctgccc atgtactcca cgtccgcatc ggacagccag gttcccgtct gggaatcaag
      541 cgacgcatca tcgacgatgg ccttggccag catcaccgag ttgatggcgc ccgccgacgc
      601 gccggcgatg acgtccagaa ccacccggaa gtgactgcgc ccatttatct ccttgagaat
      661 ctcgaaatag acggcctcgg tatctgcctg gcggttgtcg ggaccctcgg catacgaagc
      721 gtatcgggca cggccgatgc ccatctggtg aaaaaccttg gaggcgcgga cgagcttgag
      781 catctccttg gtgaccccat gcatgtagac ggcgagggaa gccccgccat atatcaccac
      841 cgcgagccgt agttctttct tcgacatggt tagccattat agtggcgcga actgcccaac
      901 ggagtacgac ccatgtcccg aatccagatc atcgccctgc tgtgctgcgc aatgctgctc
      961 gccgccccca ccatcggccg tccccaggtg gaaggaattc atgtgagcgg tcggggcgag
     1021 gtgctcgtga ccccggacat ggcccgggtc acccttcgcg tcacccgcga cgggcaggac
     1081 gccgtcgagc tcaaaaaaga gctggacgac gtgacacgca aggtgctgtc cctgacgcgc
     1141 tctctgaaga tcgagggtga ggacgtgacc gcggcggtgg tcaacatcca acctcgctac
     1201 cgccgtgggg gcggccagag cgtcatcgac ggggtcaccg catcccgcac catcgcggtg
     1261 gtattgcgcg atctcgaccg ttacggccag ctcatgaacg gcgctctgga agtgggggtg
     1321 aactcggtaa gcggtaccca gctcgactcc agcgaccggg cggcagagga agagcaagcc
     1381 ctggagcggg cgatgcagga cgcgcgcgcc gcggctgctc gcgtcgccga cggttttggc
     1441 gtctccgtgg gcaggctgct ggatgtgcac gtgggccagc ctcccgtcca gcccatgcgc
     1501 ggcgtgatga tggaggcggc ggcagccgga gacgacttca gcccgggaca gctggtcatc
     1561 tcccgccagc tgcaggccac attcgccatc gaaacccgtt gacttctgcg cggcggccaa
     1621 agactccgta tggacctctc tggagaacgg ctccgaatgc gggcagtata gccccgggaa
     1681 agtggaggac cggatcttga accacaggaa tatagtggcc gccatcggct gcctggccct
     1741 ggtcgcgtgc ggcgaagcgc ccatcgacga cgcggccccc acagcgcggc cggtgaagac
     1801 gatgatcgtc gagtctcccg ccggcagcgg cgttcgcaac ttccccggcc gaatcgattc
     1861 ggcgaacagg gcggacctgg cctttcgcgt accgggcacg gtagccaggc tcgcggcctc
     1921 ggaaggcgcg cccgtcaatc ggggacagga gctcgcgcgc ctggaccaga ccgactacga
     1981 gatcgcgctg cgtgaccgcc aggccacctg ggaccgggcc agcaaggact acgagcgctc
     2041 caaggatctg gtggacaaag gggccatctc ccggcgcgac ttcgaccggg tggaggccaa
     2101 cttcaagagc gccgatgcgg cgctggccca ggcaaagcag aacctcgagt acaccaccct
     2161 gcgcgcaccc ttcgacggca acgtggccag acggcatgtg gatgccttcg aggagatcaa
     2221 cgccggccag accgtctact ccgtgatcga ccaatccgtg ctggaagtga aaatcgacat
     2281 cccggaaaac gtcatcatga tgctgtcatc gcgcgggtcc gagcccgagg aacaggccgg
     2341 caaaatcccg gtatgggccg ccttcgacat ggccccggag cgccgctttc ctctgacggt
     2401 aaaggagtac gccacacgcg ccgacgctca gacacagacc ttcgaggtga cgttcgccct
     2461 gccggcacct gacgacgtca gggttctgcc cgggatgacg gccagcgtca ccatggatct
     2521 gtcccgggcc atggacgtgg actccgtcta ctaccttccc gtcacggccg taaccggcag
     2581 cgagggcttg accccaagag tctgggccgt ggatgaagac accatgaccg tgcgcgagca
     2641 gccgatcgtg ctggggcgcc tggtggggtc cagcgtcgag gtcaccgagg gactggagcc
     2701 cggcatgcgc atcgttaccg ccggagccgc ctacctggcg gatggcatgg cggtaacgct
     2761 gctggcccac agcgagcagg ccgagccgcg ggaagaagac ctgccgcgcc cgagctgacg
     2821 gctcgcgcgg cgctaacgga caacaacaac gatgaacatc ggctcatact ccgtcgagaa
     2881 caaggtcatc tcctggctgc tggtcatcat tctggtggcc ggcggaatct acggcttcaa
     2941 cgagatgggg aagctggagg atccgaactt caccatcaag caggcgaaga tcatcaccct
     3001 gtatcccggg gctacggctc aggaagtgca ggacgaggtg acctaccaca tcgaggaagc
     3061 gctgcagctt atgggccagc tcaagcgcat caagatgtcc atctcccgac cgggcatgtc
     3121 cgatctcgcc atcgaattca aggacgagta caaggccgac gacttcccgg acatttacga
     3181 cgagctgcgc cgcaagctgc aggacatgca cggca
//
LOCUS       DBNHKV010000289         3223 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1034221, whole genome shotgun sequence.
ACCESSION   DBNHKV010000289 DBNHKV010000000
VERSION     DBNHKV010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1034221"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..547
                     /gene="tsaE"
                     /locus_tag="AC16PP_09135"
     CDS             128..547
                     /gene="tsaE"
                     /locus_tag="AC16PP_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR6481832.1"
                     /translation="MESVFLPDAEATEAFGRDLAVRLEPGDLVLLHGDLGAGKTTLVR
                     GILAGLGHSGSVKSPTYTLLEPYELTSCVVLHFDFYRIGDSRELEFIGFDELMDADAI
                     KLVEWPERAADRLPPPDLEITLRVEDEGRRIEVEAAR"
     gene            525..1835
                     /locus_tag="AC16PP_09140"
     CDS             525..1835
                     /locus_tag="AC16PP_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR6481833.1"
                     /translation="MKSKLLVSAFLLLCYAPPSSAGKLDRVRLHEAPDHTRVVFDVAA
                     PVEFSYFTLRNPDRVVIDLKDTTAGNDFDPSRTGTKSSRVKNIRAAPRGRDYRVVLDV
                     SAPLEPKAFALEPVAAYGHRLVVDLFGAAPKPAAPAAPKADDRRDVIVAIDAGHGGED
                     PGALGPGKVREKDVVLRIARKLEARLNATNGYKAFMVRDGDYYIALRKRVDLARRSRA
                     DLFVSIHADSFRSPEVSGASVYTLSSQGATSETARWMAEKENRSDLIGGVGGVSLDHD
                     DDLLNHVLLDLSTSAKQAASHEAAKSVLGQMARVTKLHKSRVEQAGFAVLKSPDVPSL
                     LVETGYISNPAEANRLASARFQDKIASALFEGVRSYMTRSAPPGTLLAWQRESGPVRY
                     TIERGDTLSDIAVRYGTSTRRLREANGIAGDRIRVGQVIVIPAS"
     gene            1859..>3223
                     /gene="mutL"
                     /locus_tag="AC16PP_09145"
     CDS             1859..>3223
                     /gene="mutL"
                     /locus_tag="AC16PP_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010955484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR6481834.1"
                     /translation="MAGRIQVLQPFVANQIAAGEVVERPASLVKELVENSLDAGARSV
                     RLETELGGMKRVLVSDDGEGIDAADLPLAVARHATSKISGPDDLVGIGSLGFRGEALA
                     SVASVARLTITSATAEAAHGTRLEVDGPGDVHIGPAAHPRGTTVEVRDLFFNTPARRK
                     FLKTERTEQSQVDQVLRRISLCRFDVAFELRHGGTRSVLSLPAGDPELRLARVIGEAF
                     VKRSVALDETRGELRLWGWVGLPTYSRSQADQQYFYVNGRVVRDKLVAHAVRQAYRDV
                     LFGGRQPVFSLYLELPADAVDVNVHPTKHEVRFREARSVHDFIFGTLARALREVRPDA
                     VAAPVAGEAAATGSPGSWTQGRQAGLGLPHAVQDRPFPPAPRSLQEAFAEHGAPITVT
                     ESDQPIPPLGYAVAQLHGVYILAQNADGLVVVDVHAAHERVTYEKLKRQMDTEGVPQQ
                     RLLVP"
BASE COUNT          539 a         1024 c         1098 g          562 t
ORIGIN      
        1 tatgtctgca cagcgccgcc gccgatgtgg tggcaagaca agtcggtcaa cgcagcatgc
       61 tagcaacgga tcttctgccg gaagtggtgt ccatgctggc ggccgccgag cagtgagacc
      121 ggtcgcggtg gaatccgtgt ttctgccgga cgcggaagcc acggaggcgt tcgggcgcga
      181 cctggcggtc cggctcgagc ccggcgatct ggtgctgctg catggggacc tcggtgctgg
      241 aaagacaacc ctggtcaggg gcatactcgc gggtttaggt cattccggtt cggtcaagag
      301 tcccacgtat actctgctcg agccttatga attgacgtcg tgtgtcgtcc tgcatttcga
      361 tttttatcga ataggggaca gccgggaact cgagttcatc ggatttgacg agctgatgga
      421 cgcagatgcc atcaagctcg tggagtggcc ggagcgagcg gccgatcgcc tgcctccgcc
      481 tgatctcgag ataaccctgc gcgtggaaga cgaaggtcgt cgtattgaag tcgaagctgc
      541 tcgttagcgc atttctgctc ctttgttacg cgccgccatc ctcggctggc aagctggacc
      601 gagtgcgcct gcacgaggcc cccgatcaca cccgcgtggt tttcgacgtg gccgcacctg
      661 tcgagttctc ttatttcacg ttgcgcaacc cggaccgggt ggtcatcgat ctcaaggaca
      721 ccaccgcggg aaacgacttc gatccttcgc ggaccgggac taaatcgagc cgtgtgaaga
      781 acattcgcgc agcgccgagg ggacgcgact atcgggtggt gctggacgtt tccgcccccc
      841 tcgagccgaa ggctttcgcg ctcgaaccgg ttgccgcgta cggccaccgt ctggtggtcg
      901 atcttttcgg cgccgcaccg aaacccgccg cgccggctgc gccgaaggcc gatgaccggc
      961 gtgacgtgat cgtcgccatc gatgccggtc acggggggga ggatccgggc gcgctcgggc
     1021 cagggaaggt gcgtgagaag gacgtggtcc tgaggatcgc gcgcaagctc gaagcgaggc
     1081 tcaacgcaac caacggctac aaggccttca tggtgcggga cggcgactac tacatcgccc
     1141 ttcgcaaacg ggtggacctg gcacgccggt cccgggccga cctgttcgtg tcgattcacg
     1201 cggattcgtt ccgcagcccc gaagtctcgg gcgcttccgt ctacaccctc tcctcccagg
     1261 gggcgaccag cgagaccgcg cgctggatgg cggaaaagga gaaccggtcc gacctcatcg
     1321 gcggcgtggg gggcgtcagc ctggatcacg atgacgatct gctgaaccac gtgctgctcg
     1381 atctgtccac cagcgccaag caggcggcca gccacgaggc ggccaagtcg gttctcggcc
     1441 agatggcccg cgtcaccaag ctccacaaga gccgggtgga gcaggcgggc ttcgcggtgc
     1501 tcaaatcccc cgacgtgcca tcgctgctcg tggagacggg ctacatctcg aacccggcgg
     1561 aggccaaccg cctggccagc gcccggtttc aggacaagat cgccagcgcg ttgttcgagg
     1621 gcgttcgaag ctacatgacg cgctccgctc cgccgggaac gctgcttgcc tggcagcggg
     1681 aaagcgggcc ggttcgttac accatagagc ggggcgatac cctctccgac attgcggtca
     1741 ggtacggcac cagcacgcgg cgcctgaggg aagccaacgg gatcgcggga gaccggatcc
     1801 gggtggggca ggtgatcgtc attccagcat cgtgagcctt ctgcgagaat gacgggccat
     1861 ggccggtcga attcaagtac tccagccctt cgttgccaac cagatcgcgg caggcgaggt
     1921 ggtcgagcgc ccggcttctt tagtcaagga actcgtcgag aacagccttg acgccggcgc
     1981 caggtcggtt cggctggaga ccgagctggg cggcatgaag cgcgtcctcg tcagcgacga
     2041 cggcgagggc atcgatgcgg ccgacctgcc gctggccgtc gccagacacg ccaccagcaa
     2101 gatctcggga cccgacgacc tggtcggcat cggcagcctc ggtttccgcg gggaggcgct
     2161 ggccagcgtg gcgtcggtgg ccaggctgac gatcacctcg gcaacggccg aggccgctca
     2221 cggaacccgc ctggaagtgg atggcccggg agacgttcac atcggtcccg ccgcccaccc
     2281 aagggggacg acggtggagg tgcgcgatct ctttttcaac actccggccc gacgcaagtt
     2341 cctgaagacc gagcgcaccg agcagtcaca agtagatcag gtgctccggc gcatcagcct
     2401 gtgccggttc gacgtcgcct tcgagctgcg ccatggcggc acccggtcgg ttctgtcgct
     2461 gccggcagga gaccccgagc tgcgacttgc ccgggtaatt ggcgaggcct tcgtgaagcg
     2521 ctcggtggcc ctggacgaga cgcgcgggga gctcaggttg tgggggtggg tcgggttgcc
     2581 cacctattcc cgcagccagg cggatcagca gtatttctat gtgaacgggc gcgtggtgcg
     2641 agacaagctc gtcgcccacg ccgttcgcca ggcttaccgg gacgtccttt tcggtggccg
     2701 gcagccggtc ttttcccttt acctggagct gccggcggac gccgttgacg tgaacgtgca
     2761 cccgacgaag cacgaggtcc ggtttcgcga ggcgcggtcg gttcacgatt tcatcttcgg
     2821 tactctggcc cgagcgcttc gcgaggtgcg gccggatgcc gtggccgcgc ctgttgcggg
     2881 cgaagcggcg gcgaccggtt ccccggggtc gtggactcag ggtcgccagg ccgggctggg
     2941 tctgcctcac gcggtccagg atcggccttt tccgccggcc ccgcggagcc tgcaggaagc
     3001 gttcgccgag cacggcgcgc cgatcacggt cacagaatcc gaccagccga ttccgccgct
     3061 cggctatgcc gtcgctcagc tgcacggtgt ctacatcctg gctcagaacg cggacgggct
     3121 ggtcgttgtg gacgtgcacg cggctcacga gcgcgtgacc tacgaaaagc tcaagcgaca
     3181 gatggatacg gaaggcgtgc cgcagcaacg tctgctggtt ccc
//
LOCUS       DBNHKV010000290         4804 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1174134, whole genome shotgun sequence.
ACCESSION   DBNHKV010000290 DBNHKV010000000
VERSION     DBNHKV010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1174134"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="AC16PP_09150"
     CDS             <1..389
                     /locus_tag="AC16PP_09150"
                     /inference="COORDINATES: protein motif:HMM:NF019500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HIR6481835.1"
                     /translation="VGFVFFGFLSWLVTRELLFDVKEVTAETLWAAVNVYVLIGLSFA
                     FLYSVLALSSPDAFVGKFMSEPLRDQLLGFVYFSFVTLTTLGYGDITPNNTFVGTLTY
                     LEALVGQLYVAIMIARLVGLYISSQK"
     gene            516..1016
                     /locus_tag="AC16PP_09155"
     CDS             516..1016
                     /locus_tag="AC16PP_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HIR6481836.1"
                     /translation="MNGQSLLAASLIAAVAWSAPAAAQTLASSMGIFIYPTQSQTSDQ
                     QSRDDYECFGFAKTQSGYDPMNPPQITAQAPDQGPSGARVAGAARGAAGGAVIGAIAG
                     DAGKGAAYGATLGAMGGGRADRQRRAQQSQQAEQSAQQQMANMQLTFKNAYGACIEAR
                     GYSVKY"
     gene            1035..1460
                     /locus_tag="AC16PP_09160"
     CDS             1035..1460
                     /locus_tag="AC16PP_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481837.1"
                     /translation="MRTAITSCFLLPLALSGVPAAAAQFDGKQDLLCSAFRMFECDMA
                     NGCTAVTPAELGASSSWIVNAKKKTITGTNPNAQPNDIDRMELLDGKLFMSGIQDGVP
                     EERDGVAWSVSINNPDGVMTIMVAGEGLGVVGLGSCVPR"
     gene            complement(1483..2085)
                     /locus_tag="AC16PP_09165"
     CDS             complement(1483..2085)
                     /locus_tag="AC16PP_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481838.1"
                     /translation="MRAILVTVGLLLVAGCATQTTVVRADFTQGEGAYVVGYSGDPVI
                     RTRMEEQLVNDLRAREMIAFASHTDIPDITASSRYELIAAARKRGVISVIVINQVSGD
                     ASDSVVQNPERISPTHPDLQAFYDYSLGRQPVVEPGQQVFAEVNLFVLDGDEASLFWS
                     GTTWSFQADGQGTAIRGISETIADQLQKVRDQARANPFTQ"
     gene            complement(2102..>4804)
                     /locus_tag="AC16PP_09170"
     CDS             complement(2102..>4804)
                     /locus_tag="AC16PP_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6481839.1"
                     /translation="LQDMHGKLPPGVQGPRVIDDFGDVYGIYAALTGDGYTYRDLKDA
                     ADELKKQLVLVPGVRKIVIGGGQREVVYIEISRTRISELGIPLEQIANILQSQNMVTD
                     AGRVRVGPDYVRIHPTGEFTSVQAIGDVLIGSNERRLIFLKDIATIHRAYEEVANKLI
                     FFNGKPALTLGVSIQAGENVVAVGEAVRKRFGQLASTIPIGMDIKVIYNQPEEVQKSV
                     SGFIVSVGQAIAIVIAVLLVFMGLRTGIIIGAVLLITVSGTLLFMQLYGIELQRISLG
                     ALVIALGMLVDNAIVVAEGMLVRIQAGMNPTKAAEETVGKTMWPLLGGTIIGILAFSA
                     IGLSTDNTGEFASSLFYVILISLTLSWVTAVSTTPLLCAMFLKPDPDADPDANPYDKG
                     FFRTFRGALKVVIRLRVLAVAVVVGLFVLAGFGFGYIKNAFFPNSNTPMFFVDIWEPE
                     GTDIRVTRRDAQAISEFLRAQEGVRSTVALIGGGDARFALTYEPKESSPAYAQIIVQT
                     ETLEQISKIWPAVEAHLQEHYPHLDPIIKAFRIGPGRDGKIEARFHGPDPAVLRSLAE
                     RAKAIMREDPGAKEINDDWRQPVMLVRPVFNERVARQLGVTRENVATALKSAYEGTDV
                     GVYRDGIRMLPIKLRPPAEERADVANIQDIRVWSPALQTAVPIAQVVNSFETVFENTI
                     LRGRNRIQTIIAACNPTGELATPLFERLKPRIEDIELPPGYELSWGGEYEDSANAQAG
                     LARVLPAGFILMILTSILLFGKVRQPLIIWLTVPLAIIGITAGLLGFDGAFDFMSLLG
                     ALSLIGLLIKNAIVLIEEIDQQIADGKEHLTAILDSTVSRLRPVVMAASTTILGLIPL
                     LQDVFFVNMSITIMAGLGFATFLTLLFVPVLYSLFFKVAYREDA"
BASE COUNT          852 a         1499 c         1578 g          875 t
ORIGIN      
        1 tggtcggctt cgtcttcttt gggttcctgt cctggctggt gacccgcgaa ctgctgttcg
       61 acgtcaaaga ggtgacggcg gagaccttgt gggcggcagt gaacgtctac gttctgatcg
      121 ggctgagctt cgccttcctc tactcggtgc tggcgctctc gagccccgat gcgtttgtgg
      181 ggaagttcat gtccgagccg ctgcgggacc agttgctggg gttcgtctac ttcagcttcg
      241 tgaccctcac gaccctcggg tacggggata tcacgccgaa taataccttt gtcggcacgc
      301 tgacttacct ggaggcgttg gtcggccagc tgtacgtggc gatcatgatc gctcggctgg
      361 tggggctgta catctcatcg cagaagtagc ttccgggaac gggtaggtcg gagcctcatc
      421 ggaaactcgc gcttgcagtg ggcgttgcag cgatcgtata aaggcgggcc cacggggtag
      481 ggctcatgcc cactccagga ttcaaggagg actaaatgaa cggtcaatcc ctactggcgg
      541 cttcgctcat cgccgctgtg gcctggtctg cgccagcagc ggcccagacg ctcgcatctt
      601 ccatgggcat cttcatctac ccgacccaga gccagacgtc ggaccagcaa tcccgggacg
      661 attacgaatg cttcggcttt gccaagaccc agagcggtta cgaccccatg aatcctccac
      721 agatcacggc gcaggcgccg gatcagggcc cgtccggggc ccgggttgcc ggggcagcgc
      781 ggggtgctgc cggcggcgcc gtcatcggtg ccatcgccgg cgatgcgggc aagggcgcag
      841 cctacggcgc cacgctcgga gccatgggcg gcggccgagc cgaccggcag cggcgggccc
      901 agcaatccca gcaggcggaa cagtccgcgc agcagcagat ggccaacatg cagctcacct
      961 tcaagaacgc ctacggcgcc tgcatcgagg cccggggcta ctccgtcaag tattaaggga
     1021 aaggagaaga acgaatgcgg acagcgatta cttcctgttt cctcctgccg ttggccctct
     1081 caggcgtccc ggctgcggca gctcagttcg acggcaagca ggatctgctc tgctcggcgt
     1141 ttcgcatgtt cgagtgcgac atggccaatg gctgcacggc ggtcaccccc gccgagcttg
     1201 gcgcttcgag ttcctggatc gtcaatgcca agaagaaaac catcacgggc accaacccca
     1261 acgcccagcc caacgacatc gaccgcatgg agctgctcga cgggaagctc ttcatgtcgg
     1321 gcattcagga cggggtgccg gaggagcgtg acggcgtggc atggagcgtc tccatcaaca
     1381 accctgatgg cgtgatgacc atcatggtgg cgggtgaagg cctcggcgtg gtcggactgg
     1441 gaagctgcgt cccgcggtag acgccgagac cacgggtccg cctcactggg tgaagggatt
     1501 tgcccgtgcc tgatcccgca ccttctgcag ctgatcggcg atggtttcgc tgatgccgcg
     1561 aatggcggtg ccctgaccgt cggcctggaa agaccaggtg gtcccgctcc agaacaggct
     1621 ggcctcatcc ccgtccagga cgaacaggtt gacctcggca aaaacctgct ggcccggctc
     1681 gacgaccggc tgccggccaa gggaatagtc gtagaatgcc tgcaggtccg gatgcgtggg
     1741 tgagatccgc tcggggttct ggacgacgct gtcggaagcg tcgcctgaga cctggttgat
     1801 cacgatgacg ctgatcacac cacgcttgcg ggctgccgcg atgagctcgt agcggctgct
     1861 cgcggtaatg tccggaatgt cggtgtggct ggcaaacgcg atcatctccc gtgccctgag
     1921 atcgttgacc agctgctctt ccatgcgcgt ccggatcacc ggatccccgc tgtaccccac
     1981 gacgtaggcg ccctcgccct gggtgaaatc ggcccgaacc accgtggtct gggtcgcgca
     2041 gccggccacc agaagcagcc ccacggtgac cagaattgcg cgcatgtttc ctccagcgat
     2101 ctcaagcgtc ttcacggtaa gcgaccttga agaacagcga gtacagcacg ggtacgaaga
     2161 gcagtgtcag gaacgtcgcg aacccgagcc cggccatgat ggtgatggac atgttgacga
     2221 agaaaacgtc ctgcaggagc gggatcaggc cgaggatggt cgtggacgcc gccatcacca
     2281 ccggacgcag acggctcacc gtggagtcca ggatggcggt caggtgctct ttgccgtctg
     2341 cgatctgctg gtcgatctcc tcgatcagca cgatggcgtt cttgatgagg agcccgatga
     2401 ggctcagcgc gccgagcagt gacatgaagt cgaacgcgcc gtcgaaaccg aggagccctg
     2461 cggtgatgcc gatgatggcg agcggcaccg tgagccagat gatcagcggc tggcggactt
     2521 taccgaacag caggatgctg gtgaggatca tgaggatgaa gcccgcgggc agcacccggg
     2581 cgaggccggc ctgggcgttg gccgagtcct cgtactcgcc gccccaggac agctcgtagc
     2641 ccggcggcag ctcgatgtct tcgatccggg gcttgagccg ctcgaacagc ggcgtggcca
     2701 gctcccccgt ggggttgcag gcggcgatga tggtctggat gcggtttcgg ccgcgcagga
     2761 tcgtgttctc gaacacggtc tcgaacgagt tcactacctg agcgatggga accgccgtct
     2821 gcagcgccgg gctccatacc cggatgtcct ggatgttcgc cacgtcggcg cgttcttccg
     2881 ccggcgggcg aagcttgatc ggcagcatgc ggatgccgtc ccggtagacg cccacgtccg
     2941 tcccttcgta ggcggacttg agcgcggtgg cgacgttctc ccgcgtgacg ccgagctgcc
     3001 tcgcgacccg ctcgttgaac accgggcgca ccagcatgac gggctgccgc cagtcgtcgt
     3061 tgatctcctt ggcccccgga tcctcgcgca tgatggcttt cgcccgctcg gccagggagc
     3121 gcaacaccgc aggatccggg ccatggaatc gcgcctcgat cttgccgtcg cgccccgggc
     3181 cgattcgaaa ggccttgatg atgggatcca ggtgcgggta gtgctcctgc aagtgggcct
     3241 ctaccgccgg ccagattttc gagatctgct ccagggtctc ggtctgcacg atgatctggg
     3301 cgtaggcggg gctggattcc ttgggctcgt aggtcagcgc aaaccgggcg tcgccgccgc
     3361 cgatcagggc gaccgtggat cgcacgcctt cctgggctct caagaattcc gagattgcct
     3421 gggcgtcgcg tcgggtgacg cgtatgtcgg tgccctcggg ttcccagatg tcgacgaaga
     3481 acatgggcgt gttcgagttc gggaagaagg cgttcttgat gtacccgaag ccgaaacccg
     3541 ccagcacgaa cagaccgacc accacggcca ccgcgagcac ccgcaggcgg atgaccacct
     3601 tcagcgcgcc gcggaaggtt ctgaagaaac ccttgtcata ggggttcgca tcaggatcgg
     3661 catcgggatc cggcttcaag aacatggcgc acaggagggg cgtcgtgctg accgccgtca
     3721 cccaggacag cgtcagggag atcaggatca cgtagaacag ggagcttgcg aactcgcccg
     3781 tgttgtctgt cgacaggccg atggcggaaa acgcgaggat gccgatgatg gtgccgccga
     3841 gcagcggcca catggtcttg ccgaccgttt cctcggcggc cttggtgggg ttcatgccgg
     3901 cctggatgcg aacgagcatg ccttccgcta ccacgatggc gttgtccacc agcatgccca
     3961 gcgcgatcac cagggcgccc agggaaatcc gctgcagctc gatgccgtag agctgcatga
     4021 agagcaacgt gccggagacg gtgatcagca gcacggcgcc gatgatgatg ccggtgcgca
     4081 gccccatgaa gaccagcagc acggcgatca cgatggcgat cgcctgcccg acgctgacga
     4141 tgaagccgga taccgacttc tgcacttcct cgggctggtt gtagatgact ttgatatcca
     4201 tgccgatggg tatcgtgctc gccagctgcc cgaatcgctt tctcacggcc tcgcccacgg
     4261 cgaccacgtt ctcgccggcc tggatggaca cgcccagggt cagcgccggc ttaccgttga
     4321 agaagatgag cttgtttgca acctcctcgt aggcccggtg aatggtcgcg atgtctttga
     4381 ggaaaatcag acgacgctcg ttcgaaccga tgagcacgtc gccaatggcc tggacggacg
     4441 tgaactcgcc ggtgggatga atgcggacgt agtcgggtcc gacccgaacc cgtcccgcgt
     4501 ccgtgaccat gttctgggac tgcaggatgt tggcgatctg ctccagcggt atgccgagct
     4561 cgctgatccg cgttcgtgag atctcgatgt agaccacctc gcgctggccg ccgccgatga
     4621 cgatcttcct cacgccgggt accagcacga gctgcttctt gagctcgtcg gcggcgtcct
     4681 tcaggtcacg atacgtatag ccgtcgccgg tcagcgccgc gtagatgccg tagacgtcgc
     4741 cgaagtcgtc gatgacgcgt ggcccctgca cgccgggcgg cagcttgccg tgcatgtcct
     4801 gcag
//
LOCUS       DBNHKV010000291         2534 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1104318, whole genome shotgun sequence.
ACCESSION   DBNHKV010000291 DBNHKV010000000
VERSION     DBNHKV010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1104318"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="AC16PP_09175"
     CDS             complement(<1..356)
                     /locus_tag="AC16PP_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481840.1"
                     /translation="MSDDRDDLEFLRRVRGLSGEVEPERDLWPEIRAALDEPGPRAKR
                     WRLPSALAASLLLGAVAASLLFRAGTAPEPAGVPAARPALLEVGLAPELMRSRAQLIA
                     SLDAELARMSPESQAAV"
     gene            complement(356..916)
                     /locus_tag="AC16PP_09180"
     CDS             complement(356..916)
                     /locus_tag="AC16PP_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6481841.1"
                     /translation="MSMHEASSSQDTRADEALALLCQRAGAGDHGAFEELYRASVGRV
                     YGLCLRMTANVTAAEDATQATFVQAWRNLGRFRGEARVTTWLHRIAVNEVLGQQRREA
                     RHQHEPEEAWNEQASETAGAPKDLDLERAIAGLPEQARNVFVLCGVYGYGHREAAGLL
                     GIAEGTSKAHYHTARRKLREALAGAE"
     gene            990..1568
                     /locus_tag="AC16PP_09185"
     CDS             990..1568
                     /locus_tag="AC16PP_09185"
                     /inference="COORDINATES: protein motif:HMM:NF013346.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HIR6481842.1"
                     /translation="MTLDSFPPALTTFVLIGMAELGDKSQLVCMALAARHRAAPVLLG
                     ASLAFLILNALAVFLGAGLAVWLPDRLMAGLVALAFAGFGIHALTAGPGDDPEEHREH
                     RARGIFLTTLSLIFLAELGDKTQIAVAALGATLDPLQVWLGATLALFGLSALGVWIGR
                     SYLKRLPTIWLHRVSGLVFLGVGSLAAWRALT"
     gene            complement(1614..1805)
                     /locus_tag="AC16PP_09190"
     CDS             complement(1614..1805)
                     /locus_tag="AC16PP_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481843.1"
                     /translation="MVNNDLVQCLRSGARQRELDPGILDAAADALETLHRRNAELSEM
                     LVQLLPYAAADRLRHKTLE"
     gene            2095..>2534
                     /locus_tag="AC16PP_09195"
     CDS             2095..>2534
                     /locus_tag="AC16PP_09195"
                     /inference="COORDINATES: protein motif:HMM:NF024842.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR6481844.1"
                     /translation="MPADREITAEYLIIGGGALGMAIADTLIGESDSSVIMVDRRHKP
                     GGHWHDAYPFVRLHGPSANYGVNSRQLGSDRIDEAGLNRGLFELATGSEIRTYFDEAM
                     RHGLLPTGRMHYLPMHEYNGDGQAVSLISGQATKVSARKKIVDAT"
BASE COUNT          452 a          869 c          776 g          437 t
ORIGIN      
        1 accgcggcct gggactccgg cgacatccgc gccagctccg catccaggga cgcgatcagc
       61 tgcgcccggg accgcatgag ctctggcgcg agcccgacct ccagaagtgc cggacgggca
      121 gccggcacgc cggccggctc gggagccgtc ccggcgcgga atagcagcga cgcggcaaca
      181 gcgcctagaa gcagcgacgc ggcaagcgcc gaaggcagac gccagcgctt cgcgcggggc
      241 cccggctcat ccagcgctgc gcggatttcc ggccagagat cccgctcggg ctcgacctcc
      301 ccggaaagcc cgcgaacgcg ccgcaggaat tccaggtcgt cgcgatcgtc actcatcatt
      361 cagctcccgc cagcgcttct ctcaacttgc gcctggccgt gtggtaatgc gccttgctcg
      421 tgccttccgc gatacccagc aggccagccg cttcacgatg gccgtacccg tagacgccgc
      481 atagcacgaa tacgttgcgg gcctgctccg gcaagccggc gattgcccgt tccagatcca
      541 gatctttcgg cgctcccgca gtttccgacg cctgctcgtt ccaggcttcc tccggttcgt
      601 gctgatgccg tgcttcccga cgctgttgac ccagcacctc gttcacggcg atccgatgca
      661 gccaagtggt cacgcgggcc tcaccacgga agcggccaag attgcgccag gcctggacaa
      721 aggtcgcctg ggtagcgtct tccgccgcgg ttacgttcgc cgtcatgcgc aggcacaacc
      781 cgtagacccg gccaaccgat gcccggtaaa gctcctcgaa agccccatga tcgccggccc
      841 ccgcccgctg gcaaagcagc gcaagcgctt catccgcgcg ggtgtcctgt gatgaactag
      901 cctcatgcat actcatcatt atcatccctg caagataaga tgcagcctgc gcgaaaaggg
      961 tttagtcgcg tttgcggatc caggatctga tgactctcga ttctttcccc ccagccctga
     1021 ccaccttcgt gctcatcggc atggcggagc tgggcgacaa gagccagctc gtctgcatgg
     1081 cgctggcggc acgccatcga gccgctcccg tgctgctggg cgcttccctg gcttttctga
     1141 tcctgaacgc cctcgccgtc ttcctcggcg ccggcctggc cgtctggctg cccgatcgct
     1201 tgatggcggg cctggtggct ctggccttcg ccgggttcgg gattcacgcc ctgacggctg
     1261 ggcccggtga cgaccccgag gaacaccggg agcaccgtgc ccgaggaatt ttcctgacta
     1321 cgctgagctt gatattcctg gcagagctgg gagacaaaac ccagattgcc gtcgccgcgc
     1381 tcggcgccac gctcgacccg ctacaggtgt ggctgggagc taccctcgcg ctcttcgggc
     1441 tgtcggcgct cggtgtatgg atcgggcgca gctatctcaa gcgcctgccg acgatctggc
     1501 ttcaccgtgt gagcgggttg gtcttcctgg gggttggttc gctcgcggcg tggcgggcgc
     1561 tgacctgaac ggatcaaagc tcgagaccca agcccgctgc tgccagcagc gcatcactcg
     1621 agcgtcttgt ggcgcaggcg gtcggcggcc gcgtacggca ggagctgaac gagcatctcg
     1681 gacagttccg cattacgcct gtgcagcgtc tccagagcgt ccgcggccgc gtccagaatg
     1741 ccagggtcga gttctctttg acgcgcgccg ctgcgcaagc actgaacgag atcgttgtta
     1801 accacggtcg agtctccaaa taaaacggca cgcatcagtc cacgcacgcc agatgaaacg
     1861 tataggcgcg tccggttcgc catggtcggg gttaacagaa agttacccac ttaccacacg
     1921 ctcagtccgt gacgcagcgc acatagtggc gccgacgtaa tccgcagcga aggtgagaac
     1981 ccggcgcgat caaccttcgc gacccaaccc cggcttgcgt tcctttcgtc cacttgcggg
     2041 agaccgacga tccatgcaac ctgccgcggg atagtctggg caaggagcga ggagatgcct
     2101 gcagacagag aaattacagc cgagtatctc atcatcggcg gaggcgccct cggcatggcc
     2161 atcgccgata cgttgatcgg cgaaagcgac agcagcgtca tcatggtcga tcgacgacat
     2221 aagcccggcg ggcactggca cgacgcctac cccttcgtgc ggttgcacgg cccctctgcc
     2281 aactacggcg tgaactcgcg gcaactgggc agcgaccgga tcgacgaagc cggcctgaat
     2341 cggggcctct tcgagctggc cacgggctca gagatccgaa cctatttcga cgaagcgatg
     2401 cgccacgggt tgctcccgac cggccggatg cactatctgc ccatgcacga atacaacggc
     2461 gacggccagg ccgtatccct gatcagcggc caagctacca aggtgagcgc gcgaaagaaa
     2521 atcgtcgacg cgac
//
LOCUS       DBNHKV010000292         3324 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1953334, whole genome shotgun sequence.
ACCESSION   DBNHKV010000292 DBNHKV010000000
VERSION     DBNHKV010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1953334"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /locus_tag="AC16PP_09200"
     CDS             complement(<1..1367)
                     /locus_tag="AC16PP_09200"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR6481845.1"
                     /translation="MESSFDPHAIEQKLYADWEGADYFAPRGNGEGYCIAIPPPNVTG
                     SLHMGHAFQHTLMDALIRYERMKGRRTLWQMGTDHAGIATQMLVERQLLAEGLKREDI
                     GRDAFVERVWDWRNHSGNNISKQLRRMGSSLDWSRDRFTMDEGFCHAVLEVFVRLYEE
                     GLIYRGQRLVNWDPELKSVISDLEVENTEERGHLWHFRYPLAGGLTTREGKPYLVVAT
                     TRPETMLGDTAVAVHPDDERYAALVGGSVILPLADREIPIIADEYVDRDFGTGCVKIT
                     PAHDFNDNEVGARHGLALINVFNEDATINEAAPQPYRGLDRFDAREKVVADLKSQGLL
                     DGIEDHTLMVPRGDRSNAIVEPLLTHQWFVKIQPLADPAIKAVENGDIEFVPKQYENV
                     YFSWMRDIQDWCISRQQWWGHRIPAWYDKDGNVYVGRSEAAVRAEHGIPEGVPLEQES
                     DVLET"
     gene            complement(1357..1842)
                     /locus_tag="AC16PP_09205"
     CDS             complement(1357..1842)
                     /locus_tag="AC16PP_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481846.1"
                     /translation="MSTKDSADSEDELLGDLESLRSILDEEDELMPQDDEAVPLLDDV
                     VADAADAEEPARASPGGLDDDLFQALLSDDWRESAAAIIEQAPAALGQAVAWKPREIA
                     ALRQALRLKQDETLNQWISDALERHAEALQAELLEAFSGELKPAVEGILRRDAGGEHG
                     E"
     gene            complement(1842..2279)
                     /locus_tag="AC16PP_09210"
     CDS             complement(1842..2279)
                     /locus_tag="AC16PP_09210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HIR6481847.1"
                     /translation="MTRIDFYILQDVDLDAMRRFACRLAGKAITGGHEVHIHTQSAED
                     ASIVDELLWSYPDDRFIPHETLDDSGRAAVAAPVTLGWTEPDGLDGVLINLSDEIPRF
                     FGRFDRVAEVIPRSRRDAGRDRYKFYRDRGYPLFHHELDDWEA"
     gene            complement(2284..>3324)
                     /locus_tag="AC16PP_09215"
     CDS             complement(2284..>3324)
                     /locus_tag="AC16PP_09215"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR6481848.1"
                     /translation="DDAGITIRTVQVHADARTRSNVQRAVRHGNALKSGLDLARNLGN
                     QPPNVCNPPYLVREARKLARRDKVTVTSLDEKRMRELNMGAFLAVSAGSETPGRMIIV
                     RYNGGRRQDAPVVLVGKGITFDTGGISLKPPAAMDEMKYDMCGAATVLGATLAAVEAA
                     LPINLVTIVAAAENMPSGRATRPGDIVTTHSGKTVEILNTDAEGRLVLCDALSYARRY
                     EPKAIVDVATLTGACIVALGSHASAVYSKDDDLAEELVNAGEWTGDRAWRMPLWDEYQ
                     AQLKSRFADVANFGGPQAGSVTAACFLSRFTDSMRWAHMDVAGSAFQGGANKGATGRP
                     VGLLFRFLIGQS"
BASE COUNT          522 a         1077 c         1093 g          632 t
ORIGIN      
        1 caggtttcca gaacgtcgct ttcctgttcc agcgggacac cctccggaat tccatgctct
       61 gcacggactg ctgcttcgct gcggccgacg tacacgtttc cgtccttgtc gtaccaggcc
      121 ggtattcgat gtccccacca ttgctggcgg gaaatgcacc agtcctgaat gtctcgcatc
      181 caggagaagt agacgttctc gtactgcttt ggaacgaact cgatgtcacc gttctcgaca
      241 gccttgatgg ccgggtctgc cagcggctgg atcttcacga accactggtg agtcagcagc
      301 ggctcgacga tcgcgttcga gcggtctccc cggggaacca tcagcgtatg gtcctcgatc
      361 ccgtccagga gcccctggct tttcaggtcc gccaccactt tctcgcgagc gtcgaaccgg
      421 tccagcccgc ggtagggctg gggcgcggcc tcgtttatgg tggcgtcctc gttgaagacg
      481 ttgatcagcg cgagcccgtg tcgtgcgccc acctcgttgt cgttgaaatc gtgggccggc
      541 gtgatcttca cgcagccggt gccgaagtcg cgatcgacgt actcgtcggc gatgatggga
      601 atttcccggt ccgccagcgg cagaatcacc gagccgccca ccagagcggc gtagcgctcg
      661 tcgtcggggt gcacggcgac ggccgtgtca ccgagcatgg tttcgggtcg ggtagttgca
      721 accaccaggt acggcttgcc ctcgcgggtt gtcaggccgc cggcgagcgg atagcggaag
      781 tgccagaggt gcccgcgctc ctcggtgttc tcgacttcca gatccgaaat gaccgatttc
      841 agctccgggt cccagttcac caggcgctgg ccccggtaga tgagcccttc ctcgtagagg
      901 cgcacgaaca cctcgagcac ggcgtggcag aagccctcgt ccatggtgaa ccggtcgcgg
      961 gaccagtcga gagacgaacc catgcggcgc agctgcttgg agatgttgtt gccggagtgg
     1021 ttgcgccagt cccagactcg ctcgacgaag gcgtcccggc cgatgtcctc ccgtttcagc
     1081 ccctctgcca gcagctgccg ctcgaccagc atctgggtgg cgattcccgc atgatccgta
     1141 cccatctgcc acagcgtgcg tcgccccttc atgcgctcat agcggatgag cgcgtccatc
     1201 agggtgtgct ggaacgcgtg ccccatgtgg aggctgcccg tgacgttggg tggggggatg
     1261 gcgatgcagt agccttcgcc attgccccgg ggcgcaaaat agtccgcgcc ttcccaatcg
     1321 gcatacagtt tctgctcgat ggcgtgggga tcgaaactac tctccatgtt cgccccccgc
     1381 gtcgcgacgc aagatgcctt ctaccgcggg cttcagctca ccggagaagg cttcgagcag
     1441 ctcggcttgc agcgcttcgg cgtggcgctc gagtgcatca cttatccact gattcagggt
     1501 ttcgtcctgc ttcagacgca gggcttgccg aagggccgcg atctcccggg gcttccaggc
     1561 aacggcctgg ccgagggcag cgggcgcctg ctcgatgatg gcggcggccg attcgcgcca
     1621 gtcatcgctc agcagcgcct ggaacaggtc gtcgtccaat cctcccggac tggcccgggc
     1681 gggctcctcc gcgtcggccg catctgcgac cacgtcgtcc agaagcggaa cggcctcgtc
     1741 gtcctggggc atcagctcgt cctcctcgtc cagaatcgaa cgcagggatt cgaggtcgcc
     1801 cagcagctcg tcctcggagt ctgcagagtc tttggtgctc atcacgcttc ccagtcgtcg
     1861 agctcgtggt ggaacagcgg atatccgcgg tcccgataga acttgtatct atcccgtcct
     1921 gcgtcccggc gtgaccgcgg gatcacctcg gcgacccggt cgaagcggcc gaagaagcgc
     1981 gggatctcgt cgctcaggtt gatgagcacg ccgtccagac cgtcgggctc ggtccacccc
     2041 agagtcaccg gcgcggcgac ggccgcgcgg ccggagtcgt cgagggtctc gtgggggatg
     2101 aagcggtcgt cggggtacga ccagagcagc tcgtccacga tgctggcatc ctcggcgctc
     2161 tgggtatgta tgtggacctc gtggccgccc gtgatggcct tgccggcaag acggcaggcg
     2221 aatctgcgca tggcgtccag atcgacgtcc tggaggatgt agaagtcgat gcgggtcatg
     2281 gggtcaagac tgcccgatga ggaagcggaa cagaagaccc acaggccggc cggtggctcc
     2341 cttgttcgct ccgccctgga atgcgctgcc ggccacgtcc atgtgtgccc agcgcatgga
     2401 gtccgtgaac cgggatagga aacaggccgc ggtgaccgag cccgcctgag ggccgccaaa
     2461 attggcgaca tcggcgaagc ggcttttcag ctgggcctgg tactcgtccc acaggggcat
     2521 gcgccaggcc cggtcgcccg tccactcgcc ggcgttcacc aattcttcgg cgagatcgtc
     2581 gtctttcgag tacacggcgg aggcgtggga acccagagcg acgatgcagg ccccggtgag
     2641 ggttgccacg tccacgatgg ctttcggctc gtagcggcgc gcgtaggaaa gcgcgtcgca
     2701 aagcaccagg cgcccctcgg cgtcggtgtt gagaatttcc accgtcttgc ctgaatgcgt
     2761 ggtgactatg tcgcctggcc gcgtggcccg cccgctgggc atgttctccg cggccgcgac
     2821 gatggtgacc agattgatgg gcagggccgc ctccacggcg gccagggtgg cgccaagtac
     2881 cgtggcagcg ccgcacatgt cgtacttcat ctcgtccatg gccgcaggcg gcttcaggct
     2941 gataccgccg gtgtcgaagg taatgccttt gcctaccagc accacgggcg cgtcctgccg
     3001 ccggcccccg ttatagcgca cgatgatcat gcgtccgggt gtttcgcttc ctgcagagac
     3061 ggcgaggaag gcacccatgt tcagctcgcg catgcgcttc tcgtccaggg aggtgacggt
     3121 caccttgtca cgcctggcca gcttgcgggc ctcgcgcacc agatagggag gattgcagac
     3181 gttcggcggc tggttgccca gattgcgggc aaggtcgagc ccggacttca gagcgttgcc
     3241 gtgccggacg gcgcgctgga cgttgctgcg ggtgcgggca tcggcgtgca cctgcactgt
     3301 ccgaatcgtg atgccggcat cgtc
//
LOCUS       DBNHKV010000293         5364 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1174522, whole genome shotgun sequence.
ACCESSION   DBNHKV010000293 DBNHKV010000000
VERSION     DBNHKV010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1174522"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..3082)
                     /locus_tag="AC16PP_09220"
     CDS             complement(170..3082)
                     /locus_tag="AC16PP_09220"
                     /inference="COORDINATES: protein motif:HMM:NF021351.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HIR6481849.1"
                     /translation="MRPDVVKRIAGKELTLFFASPVGYLFLGAFLAFTLFVFFWVEAF
                     FARNVADVRPMFEWMPVLLVFLSAALTMRMWSEERRTGTLEFVATVPVSGWEFVVGKF
                     LACWVLLAIALLLTLPLPISVSLIADLDWGPVWAGYLAAMLLGGAYLAIGLFVSARTD
                     SQIVSLILASFACGAFYLLGSPVLTDLVGGGLADILRSVGSGSRFESITRGVLDLRDL
                     YYYASLLAVFLAANVHALEVQRWAADGGAGRHRRWLLGTGLLVANLLLANVWLAQLTG
                     LRIDVTEGRIYSVSDATRAYIGQLREPLLIRGYFSNRTHPLLAPLVPRMRDLLNEFEV
                     AGAGNIRVEIVDPADDPAAEDEANSKYGIRPVPFQVADRYESSLVNSYFDVLLSYGDE
                     YEVLGFRDLIEVKVVGEADLDVQLRNPEFDLTRAIKKVLYGFQGGGSLFANIQDPVRF
                     VGYVSSADRLPPALADYRAVLVEALDALARESGGRFTSEFIDPDAGDGSVAAQIAVDF
                     GFQPMAASLFDSNVFYFYLTLGDANTLIQLPLPEAFSVEATRRVLEEGLKRFASGLLK
                     TVALAAPEAPPYMGMGQPPQGNQYEQLKDVLRSDFEVETTDLAEGVVPDSADLLMIVD
                     PEGFNRRQLFAMDQFLMKGGTVVVATAPFSASLSQQSLMAAPRTSGLEAWLEHMGVTL
                     EPKLVMDAQNAAFPAPVTRQVGGFSFQELVMLDYPYFVDVRGEGMNAESPIVAGLPQV
                     TLTWASPIEIDAEQQEGRTVTELLTSSPRSWLSADTNVMPRLDEQGLSGFAPEGAQAP
                     RLLGALIEGRFESFFAGQTSPLLEPADGDDDNDAGADGDDETDPAATVGVVSSVIARS
                     ADSARLFLFSSDGFLTDQNLRMLGSAEGTIYTNTVQMMVNVVDWSLEDQSLLSIRSRG
                     HFNRTLPPLVDEEQLFWEYLNYGLALLGIGVVFLVHRGRKRRSDRMYASWLAGGAS"
     gene            complement(3072..3983)
                     /locus_tag="AC16PP_09225"
     CDS             complement(3072..3983)
                     /locus_tag="AC16PP_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6481850.1"
                     /translation="MIEADGLTRRYGDFVAVRNVSFSIEHGEIVGLLGHNGAGKTTIM
                     KMLTGYLEPSGGSVRVDGADIEQDRLRVQRELGYLPENLPLYPEMTVSDYLSYAATLR
                     GVDPRLAVARAVEETELEEKALDPISTLSRGFKQRVGVAQAILHEPRFVILDEPTNGL
                     DPSQTQHMRELIQRLAATATVVLSTHIMQEVDAICDRALILRDGRLVLDERLADLKRS
                     DQLRLRCAGGGPVADLLAGVSGVSRAESVAEGAWQLTVDGPLDAAAERLARALVDAGI
                     ALHELGPVQRDLETVFREVNEGVSDAA"
     gene            4181..4780
                     /locus_tag="AC16PP_09230"
     CDS             4181..4780
                     /locus_tag="AC16PP_09230"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6481851.1"
                     /translation="MKIEIPGPAGPIEAELDDANDGSGSFAVLCHPHPQYGGTMHDAV
                     LSVLGRALAGAGVGCLRFNFRGVGGSAGAHDGKGGEVEDLLAVERWLRQEHSPAHLIL
                     GGYSFGAAVAWQALTSLAPPDQMILIAPPVSFMPSTRRPVGFRLDVFAGDADQFVDAA
                     ALNVLGDAGIHIIPGADHFFGGKWGDLAAGIEQVLQPAG"
     gene            complement(4777..>5364)
                     /locus_tag="AC16PP_09235"
     CDS             complement(4777..>5364)
                     /locus_tag="AC16PP_09235"
                     /inference="COORDINATES: protein motif:HMM:NF018949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0090471 - 9,15,9'-tri-cis-zeta-carotene
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0016120 - carotene biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HIR6481852.1"
                     /translation="YQGIFSLVALVTISLLIWTYGEAPRYEYLWLPDPRLYWVTKVVM
                     VVAFISMIGGFMVKNPTAVGMEGLLKDPGQREHAATGMLRITRHPFQWSVVLWSASHI
                     VANGDTVSVVFFAAFGVLSLLGTLAIDRKKAAALGDDWEHFAAVTSNLPFAAVLSGRN
                     RLVLKELVGPGAAGLVVYALVFWGHQWVSGGVRLL"
BASE COUNT         1000 a         1843 c         1714 g          807 t
ORIGIN      
        1 tccagctcgg tttcctcacc gccgacgacc acccggtcca cgctgtccgg ggcgacctcc
       61 acaaacgccg ccggcgagct gtccgcgcca cgaccttcga ggagcaggag ggcgccggtc
      121 agcaggagct gaacggcgag cagcgcggca agccagccga tacgcgcgct catgaggcac
      181 ctcccgccag ccagctcgcg tacatccggt cggaccggcg tttcctgccc cggtggacga
      241 gaaagaccac gccgatgccc agcagcgcaa gcccgtagtt gaggtactcc caaaacagct
      301 gttcctcgtc gacgagcggc ggcagcgtcc ggttgaagtg tccgcgtgag cggatgctca
      361 acaggctctg atcttctaag gaccagtcca cgacgttgac catcatctgc accgtgttgg
      421 tatagatggt tccctccgca gagcccagca tgcgaaggtt ctggtcggtc aggaagccgt
      481 cggaggagaa caagaacagt cgggcagagt ccgccgagcg ggctatgacg ctgctcacca
      541 ccccgaccgt ggccgccggg tcggtctcgt cgtcgccgtc cgcccccgcg tcgttgtcat
      601 cgtccccgtc agcaggttcg agaaggggcg atgtctggcc ggcaaagaaa gactcgaagc
      661 gcccttctat caacgcgccg agcagccgcg gcgcctgggc accttccggg gcaaacccgg
      721 agagcccctg ctcgtcgagt ctcggcatga cgttggtgtc tgccgacagc cagctgcgcg
      781 gtgagctggt gagcagctcg gtgaccgttc gcccctcctg ctgctcggca tcaatctcga
      841 tgggcgaggc ccaggtgagc gtaacctgag gaagcccggc cacgatgggc gactcggcat
      901 tcattccttc accgcgcacg tccacgaagt acgggtagtc gagcatcacc agctcctgaa
      961 agctgaaccc gccaacctgt cgcgtcaccg gcgccgggaa cgccgcgttc tgagcatcca
     1021 ttaccagctt gggctcgagc gtcaccccca tgtgctcgag ccacgcttcc aggcccgacg
     1081 tgcgcggcgc cgccatcagg ctctgctggg acagagacgc ggaaaagggc gccgtggcga
     1141 ccaccaccgt gccgcctttc atcagaaact gatccatcgc aaagagctgt cgccggttga
     1201 acccttcagg atccacgatc atcaggagat cggcgctgtc gggcacgaca ccctcggcca
     1261 ggtccgtggt ctccacttcg aaatcgctgc gcaggacgtc cttgagctgc tcgtactggt
     1321 tgccttgggg tggctggccc atacccatgt acggcggcgc ttcgggagcc gccagggcca
     1381 cggtcttcag caacccgctg gcaaagcgct tgagcccctc ctcgagcacc cgccgggtcg
     1441 cttcgaccga gaacgcctcc ggcagcggaa gctggatgag cgtgtttgcg tcgcccaggg
     1501 tcaggtagaa atagaacacg ttggaatcga aaaggctggc ggccatgggc tggaaaccga
     1561 agtccacggc aatctgcgcg gccaccgagc catcaccagc gtccgggtcg atgaactccg
     1621 aagtgaaccg gccgccggac tcccgggcaa gcgcatccag ggcctccacg agaacggccc
     1681 ggtagtcggc caatgcgggt ggcagccggt cggcgctgga gacgtagcct acgaagcgca
     1741 cgggatcctg aatgttggcg aagaggctgc cgccgccctg aaagccgtaa aggaccttct
     1801 tgatggcgcg ggtcagatcg aactcggggt ttctaagctg aacgtcgagg tcggcctcgc
     1861 ccaccacctt gacctcgatg agatcgcgga agccgagcac ctcgtactcg tcgccgtagc
     1921 tcagcaggac gtcgaaatag gaattcacca ggctcgattc gtaacggtcc gcgacctgga
     1981 acggcaccgg ccggattccg tatttgctgt tggcctcgtc ctccgcggcg gggtcgtcgg
     2041 ccggatcgac gatctccaca cggatattgc cggcgccggc cacttcaaac tcgttgagca
     2101 ggtcgcgcat ccggggaacc aggggcgcga gcagcggatg cgttcgattg ctgaagtaac
     2161 cgcgaatcag cagcggttcc cgcagctgcc cgatgtacgc ccgggtcgcg tccgagaccg
     2221 agtagatgcg gccttcggtg acgtcgatgc gcaaaccggt cagctgcgcc agccagacgt
     2281 ttgccagcag caggttggcg accagcagcc cggttccgag cagccaacgg cggtgtcggc
     2341 cggcgccgcc gtccgcagcc cagcgctgca cctccagggc atgcacgttc gcggcgagga
     2401 aaacggccag cagcgaggcg tagtagtaga gatcccggag atccaggacg ccgcgggtga
     2461 tggactcgaa gcgcgagccg ctgccgacgc tgcgcaggat gtcggccagc ccgccgccga
     2521 cgagatccgt gagcaccggg ctgccgagca gatagaacgc accacaggca aacgaggcca
     2581 ggatcaggct gacgatctgg ctgtcggtgc gggcgctgac gaagagcccg atggccagat
     2641 aggcgccgcc gagcagcatg gcagccaggt agccggccca gaccgggccc cagtcgagat
     2701 cggcaatcaa cgagaccgat atgggcagag gcagggtcag cagcagggcg atggcaagca
     2761 ggacccagca cgccaggaac ttccccacca cgaactccca gccgctcacg ggcaccgtgg
     2821 cgacgaactc cagcgtcccc gtccggcgct cttcgctcca catgcgcatc gtcagcgccg
     2881 cagaaaggaa aaccagcagc acgggcatcc actcgaacat cggccgcacg tcggcgacgt
     2941 tgcgggcgaa gaaagcctcc acccagaaga acacgaacag cgtgaaggcc aggaaagcac
     3001 cgagaaacag gtacccgacc ggcgaggcaa agaagagcgt cagctccttg ccggcgatcc
     3061 gcttgacgac atcaggccgc atcgctcacc ccctcgttga cctcccggaa cacggtctcc
     3121 aagtcccgct gcaccgggcc gagctcgtga agcgcgatgc cggcgtccac cagcgcccgg
     3181 gccagacgct ccgccgcggc atccagcggc ccgtccacgg tcagctgcca ggccccttcc
     3241 gccaccgatt ccgcgcggct caccccggaa acgccggcga gcagatcggc cacagggccg
     3301 ccgccggcgc aacgcaggcg cagctgatcg ctgcgcttca agtcggccag gcgctcatcg
     3361 aggaccaggc ggccgtcgcg caggatcagc gctcgatcac agatcgcgtc cacctcctgc
     3421 atgatgtgcg tcgagagcac caccgtcgcg gtggccgcca gccgctggat cagctcgcgc
     3481 atgtgctggg tctggctggg atcgagcccg ttggtgggct catcgagaat cacgaacctc
     3541 ggttcgtgaa gaatggcctg agccaccccg acgcgctgct tgaagccgcg cgaaagcgtg
     3601 ctgatggggt ccagggcctt ttcttcgagc tcggtctcct ccacggcccg ggcgacggcg
     3661 aggcgcggat ccacgccccg cagggtggcc gcgtaggaca gatagtcgct gaccgtcatc
     3721 tccgggtaga gcggcaggtt ctcgggcagg tagccaagct cgcgctgaac gcgaagcctg
     3781 tcctgctcga tgtcggcgcc gtccacgcgt accgacccgc cgctgggctc gagatagccc
     3841 gtgagcattt tcatgatcgt cgtcttgccc gcgccgttgt gcccgagcag gccaacgatc
     3901 tcaccgtgct cgatggagaa ggacacgttc cgcaccgcga cgaagtcgcc gtaccgccgc
     3961 gtcaatccgt ctgcttctat catgagatca acctgccaac ccgttgccgc ttttttcgat
     4021 tccgatttcg aggccgggtt cagccccgat gccccgcccg cgcgcccgtc caaggaactg
     4081 gcgggagtgc gaaaaatagg gaaatggccg aaaaattcaa gggctgacgc cggcacctgg
     4141 ccgctggtac cgtgatccga acgcccgctg gacgctgcca atgaagatcg agattcccgg
     4201 accggccggt cccatcgagg ccgaactcga cgatgccaac gacggatccg gctcgttcgc
     4261 cgtgctctgt cacccgcacc cgcagtacgg cggcaccatg cacgacgccg ttctctcggt
     4321 cctgggccgc gcgctcgcgg gcgccggcgt aggctgcctg cgcttcaatt tccgcggcgt
     4381 aggcgggagc gcgggcgccc acgacggcaa gggcggcgag gtcgaggatc tgcttgccgt
     4441 cgagcgctgg ctgcgccagg agcacagccc cgctcatctg atcctgggcg gctattcctt
     4501 cggtgccgcg gtcgcctggc aggcgctcac cagcctcgcg ccacccgacc agatgattct
     4561 catcgcccct cccgtgagct tcatgccttc aacccgccgg cccgtcggct tcaggctgga
     4621 cgttttcgcc ggcgacgccg accagttcgt cgacgccgcc gcgctgaacg tgctcggaga
     4681 tgccgggatc cacatcatcc ccggcgcaga tcacttcttc ggtggcaagt ggggcgatct
     4741 cgccgccggg atcgaacagg tgctgcagcc ggccggctag agcaggcgca cgccccccga
     4801 aacccactgg tggccccaga acaccagggc gtagaccacc agccccgcgg caccgggccc
     4861 caccagctcc ttgagcacga gccggttgcg gccggacagc accgcggcga aaggcaggtt
     4921 cgaggtgacc gcagcaaagt gctcccagtc gtcaccgagc gcggccgcct tcttgcggtc
     4981 gatggccagg gtccccagca ggctcaggac gccgaaggcg gcgaagaaca ccaccgacac
     5041 ggtatctccg ttcgcgacga tgtgcgatgc cgaccacagg acgacgctcc actgaaacgg
     5101 atggcgggtg atgcgaagca tgcccgtggc cgcgtgctcg cgctgcccgg gatccttcag
     5161 gagaccctcc atgcccacgg ccgtgggatt cttgaccatg aacccgccga tcatggagat
     5221 gaacgccacg accatgacca ccttggtcac ccagtacagg cgcgggtccg gcaaccacag
     5281 gtactcgtat cggggagctt ccccgtaggt ccagatcagc agagaaatcg taacgagggc
     5341 aacgagagag aatattccct ggta
//
LOCUS       DBNHKV010000294         3955 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_608038, whole genome shotgun sequence.
ACCESSION   DBNHKV010000294 DBNHKV010000000
VERSION     DBNHKV010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_608038"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="AC16PP_09240"
     CDS             complement(<1..196)
                     /locus_tag="AC16PP_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6481853.1"
                     /translation="MSEAEGLGARVAALEEELGRLTDLQAIQDVLVRYSRALDWLDDA
                     MLETVFYDDADIDYGFFRGNG"
     gene            complement(231..1016)
                     /locus_tag="AC16PP_09245"
     CDS             complement(231..1016)
                     /locus_tag="AC16PP_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6481854.1"
                     /translation="MPYRRYGDSDHLLMCVSGALQTMAIWRTVVKRFADRYTVVIFDM
                     PGIGRSEIMSGGAHVTVQEQLETVHALIQETHPGGEVTLAGSSWGTAIAAAYAALRPD
                     EVQHLILSSFGMKANAGMEEIVRRALALYEGGDYARGADLILEMFGGQIGETYRRQII
                     AQFAQLNDAHAEAFAEHCRNIVKLGNLDDIIDLTRIKARTFIINGANDTIVDLEDMYV
                     AQRLIPDCQVRLIEGVGHFLHFERPELLDDYAEFMTGSAVLTS"
     gene            complement(1055..1879)
                     /locus_tag="AC16PP_09250"
     CDS             complement(1055..1879)
                     /locus_tag="AC16PP_09250"
                     /inference="COORDINATES: protein motif:HMM:NF025007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HIR6481855.1"
                     /translation="MAEFRSILHGDRPAVIDGLLNLSPDLARWTPEFLAERYGERSVR
                     VYDASFGEPGSNYMGSISSMSFAEFLNAIFGAGRDLRMFLHNVRAGFPEMLEDVVFPE
                     AGLRFSRNFVYSFFGCRGATTPLHYDIDMGCVLHTVLHGRRRIRLFALDQSTRLYRHP
                     FTVRSYVDLDRPDFQAHRALSAAQGAEVILEPGQTLFMPAGCWHEFHYLEPGFGLSLR
                     ASSPRLKDRAAGALNLLLLSPTDRLMNKLSPQAWFKWKQSIASRRAAAHLETSGQA"
     gene            2176..2958
                     /locus_tag="AC16PP_09255"
     CDS             2176..2958
                     /locus_tag="AC16PP_09255"
                     /inference="COORDINATES: protein motif:HMM:NF016974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HIR6481856.1"
                     /translation="METTPGQRRSVRDALGYLFRRAVFWFGFYIRPPIHWFIGRFSKV
                     GNPPVFDPDVFAWTPELARNWEAIRDEALAVLENPADVPPLRTVSPDHSRIATDEKWK
                     VFFLWGYGYEIEGNTVRCPVTSRLVRAVPGLISAFFSIHTPGTHLPRHYGPTNGMITC
                     HLGLQVPEDSHGCRISIDDKDYNWEPGRFLVFDDTYYHEVWNDTDEDRVVLLMHVERP
                     LRQPGKAVADGFLWLATKSPFIQATLKALDEWTATQEKDPAA"
     gene            3153..3926
                     /locus_tag="AC16PP_09260"
     CDS             3153..3926
                     /locus_tag="AC16PP_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481857.1"
                     /translation="MKVMIFTLSMLMASVAFADGHGESWSGLNLSEGSAMQMQLCSLQ
                     PRKTMADYDKVFNAYIEWSKENDVQTYAMRAMPLFGGPDPAAGLDFQWIDMLVSPFAV
                     SGNGWDKWMTTESGQKLDKQWRETANCRVAIHTVASMFLDREALTHDSRIMSLNWCTR
                     HDGVTWDNLMARHATMAAGRPDGAPVKAWTLLFPGLGVRNAPGEFAHLLSFADTAGLM
                     AWENQLANGGGWRQREDYQSSYASCTGSNVYRTTILHRP"
BASE COUNT          799 a         1255 c         1175 g          726 t
ORIGIN      
        1 ttccgtttcc gcggaaaaag ccgtagtcga tgtccgcgtc gtcgtagaac acggtctcca
       61 gcatggcatc gtccagccag tccagcgccc gggagtagcg caccagcacg tcctgaatgg
      121 cctgcaggtc cgtaagcctc ccaagttcct cttcgagcgc agcaacccgc gcacccaacc
      181 cttctgcttc actcatggtc ttctcctgga ctgcggatgc tcgacgggtt tcagctcgtc
      241 aggacggcgg agccggtcat gaactccgca tagtcgtcaa gcagctccgg ccgctcgaaa
      301 tgcagaaagt gccccacgcc ctcgatgagg cgcacctggc aatccggtat cagtcgctga
      361 gccacgtaca tgtcctcgag atcgacgatt gtgtcgtttg caccgttgat gatgaacgtt
      421 cgcgccttga tccgggtcag gtcgatgatg tcgtccagat tgccgagctt gacaatgttg
      481 cggcaatgct cggcgaacgc ctccgcatgg gcgtcgttga gctgagcgaa ctgggcgatg
      541 atctgacgtc tgtacgtctc gccgatctgc cccccgaaca tttccagaat aaggtcggcg
      601 ccccgagcgt aatctccccc ctcatacagc gccagagcgc gcctgacgat ttcctccata
      661 cccgcgttgg ccttcatgcc gaaggagctc agaatcaggt gctggacctc gtcgggcctg
      721 agcgcggcat acgccgcggc gatggcggtg ccccatgagc tcccggcaag ggtcacctcg
      781 ccgcccggat gagtttcctg aatgagcgca tggacggttt cgagctgctc ctggaccgtg
      841 acgtgcgcac cgccggacat gatctcgctg cgcccgatcc ccggcatgtc gaagatcacc
      901 accgtgtagc gatcggcgaa acgtttgacc acggttcgcc agatcgccat ggtctgcagc
      961 gcgccgctca cgcacatcag cagatggtcg ctgtccccat accggcggta aggcacctcg
     1021 aaacgtccaa tcttaagcga gcctttgact gtcctcatgc ttgacctgac gtttccagat
     1081 gggcggccgc ccgcctgctt gcgatagatt gtttccactt gaaccatgct tgcgggctga
     1141 gcttgttcat cagccggtcc gtgggcgaga gaagaagaag gttaagcgca cccgccgcgc
     1201 gatctttcag ccgcgggctg cttgcccgaa gcgacaggcc gaaacccggc tcgaggtagt
     1261 gaaactcgtg ccagcagccg gccggcatga acagtgtctg gcctggctcc aggatgacct
     1321 ccgctccttg cgccgcgctc agtgcgcgat gcgcttggaa atcgggccgg tcgagatcga
     1381 cgtagctgcg cacggtgaag ggatgacgat acaggcgggt agactggtcc agggcaaaaa
     1441 ggcgaatgcg gcgcctgccg tgaagcaccg tgtgcagaac gcaacccatg tcgatgtcgt
     1501 aatgcagcgg cgtggtggct ccccggcagc cgaagaagct gtacacgaag ttacgtgaga
     1561 accgcaggcc tgcctccggg aaaaccacat cctcgagcat ttcgggaaag cccgcccgaa
     1621 cattgtgaag aaacatgcga agatcgcgcc ccgccccgaa aatcgcgttc agaaactccg
     1681 caaaactcat tgacgagata ctgcccatgt aatttgaacc cggctcgcca aaagaagcgt
     1741 cataaacacg cacggaacgc tcgccatagc gttcggcaag aaattcgggt gtccagcgag
     1801 ccagatcggg tgacaggttc aatagcccgt cgatgaccgc cggccggtcc ccatggagaa
     1861 tggaccggaa ctctgccatg gcctctggct cgaacgaaac tctgaccacc tcgccaccca
     1921 acagataggc aggcgacgag gtcatcgagg atgtgtggta ggcggtccgg caggctgaat
     1981 gtggagcgct tcccgcgttg ataggaggca atttggctcg ggccctggag agcaggcgcg
     2041 gagtttagcc gcttttgccc ccggacccga ctcggcgtct gttacaaatt gtttcaggcc
     2101 gcatgcaagg ggcgaccttt ggccctcacg ctggtgtaat taccggctcg aatccacgag
     2161 acaggaccag aatccatgga gaccaccccc gggcagcgcc gcagtgtccg cgatgcgctg
     2221 ggctacctgt tccgccgtgc ggttttctgg ttcgggttct acatccgacc gcccattcac
     2281 tggttcatcg gacgattctc aaaggtaggc aacccacctg tcttcgatcc cgatgtcttc
     2341 gcgtggaccc ccgagctggc gcgcaactgg gaggccatcc gggacgaagc gctcgccgtg
     2401 ctggagaacc cggccgacgt gccgccgttg cgaaccgtat cacccgatca cagccgaatc
     2461 gcaacggacg agaagtggaa ggtcttcttc ctgtgggggt acgggtacga gatcgaaggc
     2521 aacacggtgc gctgccccgt gactagccgc ctggtccggg cggttcccgg gctcatatcc
     2581 gccttcttct cgattcacac gccgggcacc cacctcccgc gccactatgg cccgaccaac
     2641 ggcatgatca cgtgccatct cggattgcag gtcccggaag attctcacgg ttgccgtatt
     2701 tccattgacg acaaggacta caactgggaa ccgggccgtt ttctcgtctt cgacgacacc
     2761 tactaccacg aggtctggaa cgatactgac gaggacaggg tggttctgct catgcacgtt
     2821 gagcggcccc tgcgacagcc gggcaaagcg gtcgcagacg ggtttctctg gctggccacc
     2881 aagagcccct tcattcaggc caccctaaag gccttagacg agtggacggc tacccaggaa
     2941 aaagatccag ccgcctgacc tggctactat cgagccccgc cgtcgaccgg aagctctacc
     3001 atatgccggc ccgagcgacc cgctttaaat ttcctgcgga ggctgcaatg atgcggacgt
     3061 catcgcggca ggccgacctt cgagcaacgc gcggtacctg gggaggaacc ggcggacgcg
     3121 tagacccatc actcaatcgg ggagaaagaa aaatgaaagt aatgattttc acgctgtcga
     3181 tgctcatggc ttcagtggcc tttgcagacg gccatggcga aagctggtct ggtttgaacc
     3241 tgtctgaggg cagcgccatg cagatgcagc tctgctcgct tcagccgaga aagaccatgg
     3301 cggactacga caaagtcttc aacgcctaca tcgaatggtc gaaggagaac gacgttcaaa
     3361 cctacgccat gcgcgcaatg cccctgttcg gcggcccgga ccctgcggcc ggattggatt
     3421 tccagtggat cgacatgctg gtgtcgccct tcgcagtcag cggaaacggc tgggacaagt
     3481 ggatgaccac ggagtcgggt caaaagctcg acaagcagtg gcgggagacg gccaactgtc
     3541 gggtggccat tcacacggtg gccagcatgt ttctggaccg cgaggccctg acccacgaca
     3601 gccgcatcat gtcgctcaac tggtgcacgc gacatgacgg ggtcacctgg gacaacctca
     3661 tggcccggca cgccaccatg gcggccggcc gtccagacgg cgcgccggtc aaggcctgga
     3721 ccttgctctt ccccggcctc ggcgttcgca acgcgcccgg cgagttcgcc cacctgctga
     3781 gctttgcgga taccgcgggg ctgatggcct gggaaaatca gttagccaat ggcggtggct
     3841 ggcgccagcg cgaggactac cagagctcgt acgccagctg cacgggatcc aacgtctacc
     3901 ggaccacaat tctccacaga ccgtaacccc aaccctaacg tcacgtcgcc acgct
//
LOCUS       DBNHKV010000295         6000 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_608213, whole genome shotgun sequence.
ACCESSION   DBNHKV010000295 DBNHKV010000000
VERSION     DBNHKV010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_608213"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="AC16PP_09265"
     CDS             complement(<1..723)
                     /locus_tag="AC16PP_09265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6481858.1"
                     /translation="MNFGFTEEQNMLRDQVARFMQEACPIPKVREIGASQTGFDAALW
                     QQAAQLGWLSLIIPEENGGLGLRWIDLTVVLEETARGLCPLPIASQAMTAAAILRCGS
                     DSQKSSWLPAMAAGEVVCTTALYDDPNWIHPDAVQLTATPVEGGVRLDGVKPFVPDAL
                     AADYLLLAYRGPDGLGLAALSRDQVTFTPQPQMDSTKPAGRVVMDAVTVGDEFLLPLS
                     EADLDYLTDVGAVAVTAEMVGAA"
     gene            complement(720..1922)
                     /locus_tag="AC16PP_09270"
     CDS             complement(720..1922)
                     /locus_tag="AC16PP_09270"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6481859.1"
                     /translation="MSGPLEGFKVIDLSAVVSGPLTGALLADLGADVIKVERTVTGDI
                     QRNVGSRRNGFSGFFHVLNRGKRSIALNLTTREGCDIVHRLSADADVVLQNFRPGVVE
                     RLGIDYARLCADNPGLVYLSISGFGQSGPRAKDRAYDPIIQAYAGITDVQGSIHRDNP
                     DQPEQVNMLLLDKLTAWTGCQAIMAALLARSRTGKGQHVELSMLDTAISFIWSDVAAD
                     LILQGDDVDRRPPIRASGTMQTYADGHGFTMTLSQPEFEGLCRAYGLDHIAEDARFAS
                     LDLRMRHRAELREIISSEVDEVALELTVAQAEERLLRHEVPFARVRRLADLPEDEQIQ
                     HNQMFRELNHPVAGPLRDARPAPRFASTPAEPGGPAPSVGEHTRQILEEISMGAETEK
                     LFERGIVS"
     gene            complement(1933..2799)
                     /locus_tag="AC16PP_09275"
     CDS             complement(1933..2799)
                     /locus_tag="AC16PP_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6481860.1"
                     /translation="MLDGKVAVVTGAGGGIGRSHAKLLAKEGAAVVVNDLGGARDGTG
                     ASQSMADTVVDEIIADGGRAIANYGSVSDKAAAQAMIDAAVSEFGRIDIMIANAGILR
                     DKSFKNMDDEMWDVVLDVHLRGTYLTVKSAYDRMLEQGEGGRIIMTSSTSGLLGNFGQ
                     TNYGAAKAGIAGFMRCLWLEGLKYGINVNVLVPTATSRLTEDILPEDIQDRFPPETVS
                     PAIVWLCTDEAKEVTGRQWLVAGNNVSLLSWQITPIADRDVSEGPWDVADIGQRILAS
                     KESWPPINPLRG"
     gene            complement(3017..3883)
                     /locus_tag="AC16PP_09280"
     CDS             complement(3017..3883)
                     /locus_tag="AC16PP_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014951.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR6481861.1"
                     /translation="MPEILFLLGEAALARNDNHVRLPEAFAQAGWQVTALPHDCLGVH
                     AGRIHLDGHDPDRFDRIWPLGLGPRETFFDRMQMLAALDQRRLVIGADAFTYLHGKYR
                     WLDLMPETHAANDAEALARVIASGGDWVIKPPAGSYGQGVQVIREGEDPMPALRALTG
                     GEARRYCLVQRYVPEIVRGEKRTLVAGGEVCGTYLRTPTKDHRANLSAGGRASAAKLT
                     EEEARLVEGLTAELLSLGAAFAAIDIVAGRLMEVNVANPGGLATLENVYGRDPTPQAV
                     AALMAYWARTGG"
     gene            complement(3890..4492)
                     /locus_tag="AC16PP_09285"
     CDS             complement(3890..4492)
                     /locus_tag="AC16PP_09285"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6481862.1"
                     /translation="MYTLYEMGDSGNCYKIRLLMHQLDVPFERVPTDILQGRSRTESF
                     LALNPNGKVPLLVLPDGRPLAESNAILSYLARGTAYLPDEAYEQALVLQWLFFEQYSH
                     EPYIATNRFWIHILKDPVGHAQEIAARHPKGEAALGVMETHLAAEEFFVAGRYTIADI
                     ALYAYTHLAHQGGYDLDPFPAVRAWLARVKAQPRHSPIDA"
     gene            4604..5653
                     /gene="nadA"
                     /locus_tag="AC16PP_09290"
     CDS             4604..5653
                     /gene="nadA"
                     /locus_tag="AC16PP_09290"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR6481863.1"
                     /translation="MSEATLPDYTLSADRVPVYEPEVLGPERVADLKSEIADLLAARG
                     AVLVAHYYVDGHVQDLAEETGGFVSDSLEMARFGHDHAAETLVVAGVRFMGETAKILS
                     PEKRVIMPTLEAECSLDIGCPPDDFEAFCKANPGRTVVVYANTSARVKAMSDWVVTSS
                     VALPIVEMLDARGEKILWAPDKHLGHYIQTQTGADMLLWSGACVVHEEFKSKALFDLK
                     AVYPEAGVLVHPESPAAVISIADVVGSTSQIIKAAQELPHDTFIVATDRGIFHKLRQR
                     NPDKRFIEAPTAGEGATCRSCAHCPWMAMNELSTLAAMLRSEGAMTANEIHVDPEIGR
                     RAMIPLKRMLDFKVP"
     gene            complement(5650..>6000)
                     /locus_tag="AC16PP_09295"
     CDS             complement(5650..>6000)
                     /locus_tag="AC16PP_09295"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6481864.1"
                     /translation="LALNPDNATLAAMYSEALIRDQRYREAEAVLERQSVVNKDDVDV
                     WYNLAEVSGKAGNIIGVHRARAEYFALHGAYQRAIQHLEYARRLVREDNQQLHAMLDQ
                     RINDMRMALRMRRS"
BASE COUNT          987 a         1954 c         1989 g         1070 t
ORIGIN      
        1 ggcggcaccc accatctccg ccgtcacggc gacggcgccg acgtccgtga ggtagtcgag
       61 atccgcctcg ctcagcggca gcagaaactc atcgccgacg gtgaccgcgt ccatgaccac
      121 acgtccggcc ggcttggtgc tgtccatctg cggctgcggg gtaaaggtga cctggtcgcg
      181 gctgagtgct gccagcccca gcccgtcggg ccctcggtag gccagcagca ggtagtcggc
      241 ggcgagagcg tcgggcacga aaggcttgac gccgtccagg cgcacgccgc cctccacggg
      301 cgttgcggtg agctgcacgg cgtcgggatg aatccagttc ggatcatcgt acagcgccgt
      361 ggtgcaaacg acttccccgg ccgccatggc tggcagccag ctcgatttct gcgagtcgct
      421 gccacatctg aggatggcgg cggcggtcat tgcctgggag gcgatgggca gcgggcaaag
      481 tccccgggcc gtttcctcca gcaccacggt cagatcgatc cagcgcagtc caaggccgcc
      541 gttctcctcg gggatgatga ggctcagcca gccgagctgc gccgcctgtt gccaaagagc
      601 cgcatcgaag ccggtctgtg aagcaccgat ctcgcgcacc ttgggaatgg ggcaggcctc
      661 ctgcatgaag cgcgcgacct ggtcgcgcag catgttctgc tcctcggtga agccaaaatt
      721 catgaaacga tacctcgctc gaacagtttc tcggtctcgg cgcccatgct gatttcctcc
      781 agaatctgtc gcgtgtgctc tcccacgctc ggcgcgggcc cgccgggctc cgccggggtg
      841 ctggcgaatc gcggggcggg cctcgcgtcg cgcaatggtc ccgccacggg gtgattcagc
      901 tcccggaaca tctggttgtg ctggatctgc tcgtcttcgg gcaggtcggc caggcgccgc
      961 acccgggcga aaggaacctc gtgacgcagc agcctctcct ccgcctgcgc caccgtgagc
     1021 tccagcgcca cctcgtcgac ctcggagctg atgatctcgc gcagctcggc gcggtgccgc
     1081 atgcgcaggt ccaggctggc gaagcgggcg tcctcggcga tgtggtccag gccgtaggca
     1141 cggcacaggc cctcgaactc cggctggctc agcgtcatcg tgaagccgtg gccgtcggcg
     1201 taggtctgca tggtgccgga agcgcggatg ggcgggcgcc tgtcgacgtc gtccccctgc
     1261 agaatcaggt cggcggccac gtcggaccag atgaacgaga tggcggtgtc cagcatggac
     1321 agctccacgt gctggccctt gcccgtgcgg ctgcgtgcca gcagagcggc catgatcgcc
     1381 tggcacccgg tccaggccgt cagcttgtcc agcagcagca tgttcacctg ctcgggctgg
     1441 tcgggattgt cccgatgaat gctcccctgc acgtcggtga tgccggcgta ggcctggata
     1501 atggggtcgt aggcccgatc tttggcccgg ggcccggact ggccgaaccc gctgatggaa
     1561 aggtagacga gccccggatt gtcagcacaa agcctcgcgt aatcgatgcc caggcgctcc
     1621 accacaccgg gcctgaagtt ctgcagaacc acgtcggcgt ccgcggagag gcggtggacg
     1681 atgtcgcagc cctcccgggt ggtgagattc agcgcgatgg accgcttgcc tcggttgagc
     1741 acgtgaaaga atccggaaaa accgttccgg cgactgccca cgttgcgctg gatgtcgccg
     1801 gtgaccgtcc gctcgacctt gatgacatcg gcacccaggt cggcgagcag ggctccggtc
     1861 agcggcccgg agaccaccgc ggacagatcg atgaccttga agccttcgag gggaccggac
     1921 atggagcctg cctcagcccc ggagaggatt gatcggcggc cagctctcct tgctcgccag
     1981 gatcctctgc ccgatgtccg ccacgtccca cggcccttcc gagacgtccc ggtcggcgat
     2041 gggggtgatc tgccaggaga gcagcgagac gttgttgccg gcgaccagcc actgccggcc
     2101 ggtgacctcc ttggcctcgt ccgtgcacag ccagacgatg gccggcgata cggtctccgg
     2161 cgggaatcgg tcctggatgt cctcggggag gatgtcttcg gtgagccgcg acgttgcggt
     2221 gggtacaagc acgttgacgt tgatgccgta cttcagcccc tcgagccaga ggcagcgcat
     2281 gaatcccgca atgccggctt tggccgcgcc gtagttggtc tggccgaagt tgcccagcag
     2341 ccccgaggtg gagctggtca tgatgatccg gccgccctct ccctgttcga gcatccggtc
     2401 gtaggcggat ttgacggtga ggtaggtgcc acgaagatga acgtcgagga ccacgtccca
     2461 catctcgtcg tccatgttct tgaaagactt gtcgcggaga atgccggcgt tggcgatcat
     2521 gatgtcgatg cgcccgaact cgctgacggc ggcatcgatc atggcctggg ctgcggcctt
     2581 gtcggagacg gagccgtagt tcgcgatcgc ccggccgccg tctgcgatga tctcgtccac
     2641 gacggtgtcg gccatggact gggatgctcc cgtgccgtcg cgggcgccgc ccaggtcgtt
     2701 gaccaccacg gcggcccctt ccttggccag cagcttggcg tgggatcgtc cgataccgcc
     2761 gcctgcgccg gtcaccacag caactttgcc gtcgagcatt cccatatcga tctccttatt
     2821 ctgtgcgatt cagcagtccg gtcggcagga agcgtagggg aggtgcttgc tgcagtgcgt
     2881 gctgcaacgg ttgacgcgcc cgagcggcgt tgcctgccgc aggctgagcg gaacgttcaa
     2941 acccccagtc cttcgcccgc cgaatcggac gaacggtgca gtctaaggcg aagcgccgca
     3001 aacgcaagcg ccgggctcag ccgccggttc gggcccagta ggccatgagc gcggccacgg
     3061 cttggggcgt cgggtcgcgt ccgtagacgt tctcgagggt ggccaacccg cccggattgg
     3121 ccacgttaac ttccatcagc cggccggcca cgatgtcgat ggctgcgaac gcggcgccga
     3181 ggctcagcag ctcggccgtc agcccctcga cgagcctggc ttcctcctcc gtcaacttgg
     3241 cagcgctcgc ccgaccgccc gcgctcaggt tggcacgatg gtctttcgtg ggtgtgcgca
     3301 ggtaggtgcc gcagacttcc ccgcccgcta ccagggtgcg cttctcgccc cggacgatct
     3361 cggggacgta gcgctggacc aggcagtaac gtcgcgcctc gccgcccgtg agggcgcgca
     3421 gcgcgggcat ggggtcttca ccttcccgga tcacctgcac cccctgaccg tagctcccgg
     3481 cggggggctt gataacccag tcgccgccgc tggcgattac tcgtgccagg gcctcggcgt
     3541 cgtttgcagc atgggtctcg ggcatgaggt ccagccaccg gtacttgccg tgaaggtagg
     3601 tgaaagcatc ggcgccgatc accagacgtc gctggtccag cgccgcgagc atctgcatgc
     3661 ggtcgaagaa cgtctccctg gggccgagcc cgagcggcca tatccggtcg aagcggtcgg
     3721 ggtcgtgccc atcgaggtgg atcctgccgg cgtgaacgcc caggcagtca tggggcaggg
     3781 cggtgacctg ccagcccgcc tgggcgaacg cttcgggcaa ccgcacgtgg ttgtcgttgc
     3841 gggcgagtgc cgcctctccc agcagaaaga gaatctccgg cacgggcggt caggcgtcga
     3901 tgggactgtg gcgcggctgt gccttgaccc gcgcaagcca ggccctaacc gccggaaagg
     3961 gatccaggtc gtagccaccc tggtgggcga ggtgggtgta ggcatacagg gcgatgtccg
     4021 cgatggtgta gcggccggcg acgaagaact cctccgcggc gagatgggtt tccatcactc
     4081 caagggccgc ctcgcccttg ggatgtctgg ccgcaatctc ctgcgcgtgg ccgacggggt
     4141 ccttgagtat gtgaatccag aatcggttgg tggcgatata gggctcatgg ctgtactgct
     4201 cgaagaacag ccattgcagc accagcgcct gctcgtaagc ctcgtccggg aggtaggccg
     4261 tacctcgcgc aaggtagctc agaatggcgt ttgactccgc cagcgggcgc ccgtcaggta
     4321 gaacgagcag cgggaccttg ccattgggat tcagcgccag gaacgattcc gtgcgcgacc
     4381 gcccctggag gatgtcggtg ggtactcgct cgaaaggaac atccagctgg tgcatcaata
     4441 gccggatctt ataacagttt cccgagtctc ccatctcgta aagcgtgtac atcgaacctc
     4501 cttggttttc gtcgattcgc cccaataaaa agatggtcat ataaacaggc gacggttgca
     4561 cgggaactgt gattttccct gcgcttccaa tattctcgat gtcatgagcg aagctacgct
     4621 gccagactac accctctccg cggaccgggt acccgtctac gagccggagg tgctcgggcc
     4681 ggaacgggtt gcagatctga aatccgagat tgccgatctg ctcgccgccc gcggcgccgt
     4741 gctggtggcc cattactacg tggacggtca cgttcaggac ctcgcagagg aaacgggcgg
     4801 gttcgtctcc gactcgctgg aaatggcccg attcgggcac gaccacgccg ctgagacgct
     4861 ggtggtcgcg ggcgtgcgtt tcatgggcga gaccgccaag attctctcgc cagagaagcg
     4921 ggtgatcatg ccgacgctcg aggccgagtg ctctctggac atcggctgcc cgccggacga
     4981 cttcgaggcc ttctgcaaag ccaatcctgg ccggacggtg gtcgtctacg ccaatacctc
     5041 ggcccgggtc aaagcgatgt ccgactgggt ggtgaccagc agcgtggccc tgcccatcgt
     5101 ggagatgctc gacgcccgag gtgaaaagat actctgggcc cccgacaagc atctgggaca
     5161 ttacattcag actcagaccg gcgccgacat gctgctgtgg agcggcgcct gtgtggtcca
     5221 cgaagagttc aagtcgaagg cgctcttcga cctaaaggcc gtttatccgg aggcgggcgt
     5281 cctcgtgcac cccgagtcac cggccgcggt gatttccata gccgacgtgg tgggctcgac
     5341 cagccagatc atcaaggcgg cccaggagct gccccacgac accttcatcg tggccaccga
     5401 ccgcggcatc ttccacaagc ttcgccagcg caacccggac aagcgattca tagaagcgcc
     5461 cacggccggc gagggcgcaa cctgccgcag ctgtgcccac tgcccttgga tggcgatgaa
     5521 tgagttatcc accctggcgg ccatgctgcg cagcgaaggg gcgatgacgg ccaacgagat
     5581 tcatgtggac cctgagatcg gtcgtcgggc gatgatcccg ctcaagagga tgctcgactt
     5641 caaggtgccc taggaccgcc gcatccgcag cgccatccgc atgtcgttga tgcgctgatc
     5701 cagcatggcg tgaagctgct ggttgtcttc ccgcaccagg cgccgcgcgt attccagatg
     5761 ctggatggcc cgctggtagg cgccgtgcag cgcgaagtac tcggcgcgag cccggtgcac
     5821 gccgatgatg ttgcccgctt tgcctgatac ctcggcgagg ttgtaccaca cgtccacgtc
     5881 gtccttgttg acgacgcttt gccgctccag caccgcctca gcctcccggt accgctggtc
     5941 cctgatgagc gcctcgctgt acatggccgc cagggtggcg ttgtccgggt tgagggccag
//
LOCUS       DBNHKV010000296         1896 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1671612, whole genome shotgun sequence.
ACCESSION   DBNHKV010000296 DBNHKV010000000
VERSION     DBNHKV010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1671612"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="AC16PP_09300"
     CDS             <1..621
                     /locus_tag="AC16PP_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6481865.1"
                     /translation="GYGGSKHIAGEHSVAEIAADCLGVADALGWGEFHVVGHSMTGMA
                     TQRLALEAPERDRSAVAVCPVSAAGMAMDDQTRAFFESTTTDDEAFRGLIGMISGSLS
                     GGWVDAKLRWNREAAAPDVRLDYLAMFADTDFAESVEGLETPFLVMVGEHDAEGLDAA
                     AMQKTYLAWHPNAELVVIPNCGHYPMQECPPFFVSTLESFLRRRAG"
     gene            complement(646..1509)
                     /locus_tag="AC16PP_09305"
     CDS             complement(646..1509)
                     /locus_tag="AC16PP_09305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6481866.1"
                     /translation="MEFGAVFPHNEIGTDPGAIKAYAQGVEAMGATHLLIYDHVLGAD
                     PNRPGGWKGAYDKDVAFHEPFTTFAFIAAVTDKLEMITTVLILPQRQTVLVAKQAAEV
                     AILSNNRFRLGVGSGWNQVEYVGLNEDFHTRGRRQAEQVELMRKLWSEDSLTFDGAFH
                     TIDAASINPRPGASVPVWFGGSAAPLLKRCAELGDGWIPLMGANPKARECIDTIRAHR
                     EAAGLPWEGFGIQAQAQYAGGNPDRWASHAGKWQDMGCTHLAIATHNAGPTNADGHLR
                     RIEEYLQAVAA"
     gene            1613..>1896
                     /locus_tag="AC16PP_09310"
     CDS             1613..>1896
                     /locus_tag="AC16PP_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012361.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR6481867.1"
                     /translation="MADPAQSSSSFSFVDMEHQILDFWEQGRVFQRSLENTREKKPYI
                     FYDGPPFATGLPHHGHIVASTIKDIVPRYWTMKGRYVLRRFGWDCHGLPI"
BASE COUNT          303 a          605 c          628 g          360 t
ORIGIN      
        1 ggttatggcg gctcgaaaca catcgccgga gagcactcgg tggcggagat cgccgccgat
       61 tgcctgggtg tggcggacgc cctgggctgg ggtgagtttc acgtggtcgg tcactccatg
      121 acgggcatgg cgacccagcg gcttgccctg gaggccccgg agcgcgacag gagcgcggtg
      181 gccgtctgcc ctgtgtccgc agccggcatg gccatggatg atcagacgcg ggcgttcttc
      241 gagagcacca ccaccgacga tgaagccttt cgcgggctga tcggcatgat cagcggctcg
      301 ctttcgggcg gttgggtgga cgcgaagctg cgctggaacc gcgaggcggc ggccccggac
      361 gtgcggctcg actatctcgc catgtttgcc gataccgact ttgcggagtc ggtggagggg
      421 ctcgaaacgc cgtttctggt gatggtcgga gagcacgacg cggaaggtct cgacgcagcc
      481 gccatgcaga aaacctacct tgcctggcac cccaatgcgg agctggtggt gattccgaac
      541 tgcggtcact accccatgca ggagtgtccg ccgttctttg tcagcactct ggagtctttc
      601 ctgcgcagac gggcgggctg atcgtcctgc cagctcccgg cggcgtcagg ctgccacggc
      661 ctgaaggtat tcctcgatgc ggcgcagatg accgtccgcg ttcgtcggcc ccgcgttgtg
      721 ggtggcgatg gccaggtggg tgcagcccat gtcctgccac ttgcctgcat gggatgccca
      781 tcgatcgggg ttgccgcccg catactgggc ctgggcctga ataccgaatc cctcccaggg
      841 cagcccggcg gcttcgcggt gagcacggat cgtgtcgatg cattccctgg ccttcggatt
      901 cgcgcccatg aggggtatcc agccgtcgcc gagctcggcg cagcgcttca acagcggcgc
      961 ggcgctgccg ccgaaccaca ccgggacgct ggcgccgggg cgcggattga tgctggcggc
     1021 gtcgatggtg tgaaaggcgc cgtcgaaggt caggctgtcc tcgctccaga gcttgcgcat
     1081 gagctcgacc tgctccgcct ggcgcctgcc ccgggtatgg aagtcttcgt taagccccac
     1141 gtactccacc tgattccagc cgctacccac gcccaggcgg aaccggttgt tggagagaat
     1201 ggccacctcg gccgcctgct ttgcgaccag caccgtctgc cgctggggca agatcagcac
     1261 cgtagtgatc atctcgagct tgtcggttac cgcggcgatg aaggcgaacg tggtgaaggg
     1321 ttcgtggaaa gccacgtcct tgtcgtacgc ccccttccag ccgcctggac ggtttggatc
     1381 cgcgcccagc acgtggtcgt agatcagcag atgggtcgcg cccatggctt ccactccctg
     1441 ggcgtaggcc ttgatggcgc cggggtccgt tccgatctcg ttgtgcggaa atacggctcc
     1501 gaattccatt gacatcccct cgagtcctgc ggcgaacggg cgagcttagt gccctacgga
     1561 atggcgcgca atggcgttac aatgcgcggc cgttttcgac cgcatgaccc caatggccga
     1621 cccagcacaa tcgagctcat cgttctcgtt cgtggacatg gagcaccaga ttctggattt
     1681 ctgggagcag ggccgtgtgt tccagcgcag cctggagaac acgcgggaga agaaacccta
     1741 catcttctac gacggtccgc cctttgcgac cggtcttcct caccacggtc acatcgtggc
     1801 cagcaccatc aaggacatcg ttccgcggta ctggacgatg aaaggccgct acgtgctgcg
     1861 ccgcttcgga tgggactgcc acggcctgcc catcga
//
LOCUS       DBNHKV010000297         7030 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_891973, whole genome shotgun sequence.
ACCESSION   DBNHKV010000297 DBNHKV010000000
VERSION     DBNHKV010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7030
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_891973"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="AC16PP_09315"
     CDS             <1..847
                     /locus_tag="AC16PP_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6481868.1"
                     /translation="ALVAAALDLGVNFIDTAPAYRTEGILGRALRGRRESAVVSTKAL
                     IHLNTEHPPKPSRFITGAELEARLDRSLEALGTDYVDVYNLHGIVEPELDYVDAELVP
                     KLLDLKAAGKIRSLGITEHFQVDTRHEMLARAVRSGVWDVVMVGFNLLNPCARQNVLA
                     PARAMNVGVLNMHAVRTSLSDPDRLTEALETAGDAGQLDLPPAGTAAALQSLVSESGA
                     GSLTELAYRFCRHEPGVHVVLTGTGKLDHLRANIAAIDNDPLPAPVSAGLEGLFGSVD
                     CLSGE"
     gene            complement(859..1584)
                     /locus_tag="AC16PP_09320"
     CDS             complement(859..1584)
                     /locus_tag="AC16PP_09320"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6481869.1"
                     /translation="MSRLRDKVCLITGGARGQGAAEARAFAREGAKVWIADVLEDEGS
                     KLAAETGGEFRVLDVTSESGWQALVDEIIAVDGRIDVLVNNAGIYRANRLIHTTLDEY
                     EQVMAVNATGVFLGLRAVGREMKKAQAGSIVNISSYAGMAAAAGSFAYGASKWAVRGM
                     TKTAAVELARSGVRVNSIHPGAIDTEMLSEAGDPAKLVRGTPLGRVADADEVASMALF
                     LASDESSYSTGAEFLVDGGLFAI"
     gene            complement(1637..3421)
                     /locus_tag="AC16PP_09325"
     CDS             complement(1637..3421)
                     /locus_tag="AC16PP_09325"
                     /inference="COORDINATES: protein motif:HMM:NF018938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HIR6481870.1"
                     /translation="MSDVIRIGNCSGFYGDRLEAAREMVEGGPIDVLTGDYLAELTMS
                     ILYSQIQSRGEDMGYVGTFLKQVRDVVAICIERGIKIVSNAGGLNPRSMAAEVDKILA
                     ELGLEATVAYIDGDDLLPDLERLQAEGEPFTNLDTGMALAETDEKALTANVYFGGWGI
                     KEALDRGADIVICPRVTDAAVVLGPAAWKFGWARNDYDALAGALAAGHIIECGAQCCG
                     GNYSFFEEVPSFRNVGYPIAEIEADGSFTITKHPGTGGLVSVGTVTAQLLYEISTPAY
                     FNPDVVAHFDTMTIEQTGEDRVRVSGCRGSSPPPTHKVCFNTLGPCKQSLEVLLTGLD
                     IEKKAEIYVDQVFHNLGGREQFDDVDVQLIRSDKEDPATNEEAHAALRITVTTRDPAK
                     LGRLLQAKVTELALAAIPGNTGRGAAGFNGSPVTVHWPALVDSRHVTERVHVGGATTD
                     VLPTQRLGLEEIYYQEVPVDVAPAPEGDRVTIPFGRLFGTRSGDKGGNANCGVWARTD
                     DAYGFLHRYLTVDQLKKLLPDMAGYEIERYDLPNLRAMNFYIRGVLGTGAASNHRIDK
                     QAKSLGEYLRAKLIEVPDALVAELARAE"
     gene            complement(3414..4202)
                     /locus_tag="AC16PP_09330"
     CDS             complement(3414..4202)
                     /locus_tag="AC16PP_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6481871.1"
                     /translation="MAGTQATLYETRKGAAWITLNRPANRNALSAPLVNELYDHLLVA
                     NDDAAVRSIVITGTGPAFCAGADLKEPPGQAIDGGRAVPYADVLSAILDSPKPVIAAV
                     NGAAFAGGLGLIGAADIVVTAEDVQFSFSEVRIGVIPAVISVVCLPKLGVHHGMKLIL
                     TGERFSGRRAVEIGFAHRAVPADELVDAVQEEIDMINLGGPNAMVECKKLVRRVPRLT
                     VEDGFREAAEWSSRMFRSDEAAEGMAAFREKRKPAWAVGEGEDE"
     gene            complement(4218..6191)
                     /locus_tag="AC16PP_09335"
     CDS             complement(4218..6191)
                     /locus_tag="AC16PP_09335"
                     /EC_number="6.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HIR6481872.1"
                     /translation="MADVSRPISRILVANRGEIARRIIRTAHELGIGTVAVYADADAD
                     APYVREADTAIALGGRSSTETYLDIPKVIEACRQSGADAVHPGYGFLSENERFAQAVV
                     DEGVRWIGPAPRVIGLMGDKLSAKRLMVEAGVPTLPAVEITEATDLAEAASQIGYPVL
                     VKASAGGGGRGMRVVEDEGGLADAVLGAKREAASSFGDDTVFFEKWLESSRHVEVQIL
                     GDGHGNVLHCFERECSIQRRHQKIIEEAPSPAVTPDLRARMGAAAVAAAKKLGYTSAG
                     TVEFLLSGDEFYFLEVNARLQVEHPVTEAVIGWDLVREQIRVAEGERLSFTQEELAIS
                     GHAIQARLYAEDPGRDFLPSPGTVVAWAPSRAGSARFDSGVESGSVIGTEFDPMIAKV
                     IVHAPTRREAATRLARVLETTRIQGLTTNRDFLVATLRTPEFLAGDTTTDFIERVRPA
                     VVRCIGRDELVNAAIAAAIEGRDARRKSARVMGTIPGGWRNSTMPMETVGYSAGAGEV
                     QLRYRARRDGMFRFVADESEKLVRVHGAGGAETDLEINGQRLRFLVGRLGDTWMVHGS
                     SGNVELVELPRYPLAGGGDLGSGMLAPMPGRVLETQVAEGTEVVKGQLLLVLEAMKME
                     HRITAPRDGVVAKVHVNAGDQVENGQLLVTLAE"
     gene            complement(6179..>7030)
                     /locus_tag="AC16PP_09340"
     CDS             complement(6179..>7030)
                     /locus_tag="AC16PP_09340"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR6481873.1"
                     /translation="DGIRMCREVVKHLNWRKLGPEPRADFAEPLHDPEDLLGMVSKDL
                     RSPFDMREVIARIVDASYFEEFKPLYGPTLVCGWASIHGYPVGILGNNGALFSESSEK
                     AAQFIQLCNQIDVPLLFLHNITGFIVGTDFEQGGITKNGSKMINAVANSTVPHITVIV
                     GASYGAGNYGMSGRAFGPRFTFIWPFAKIAVMGPKQMAGVMSIVGRAAAARRGIEFDE
                     EADASRAAVAEHWAEERSKGLYATSRVSDDGMIDPRDTRTVVAIALSACFNNRVEGTK
                     EFGTWRM"
BASE COUNT         1153 a         2481 c         2187 g         1209 t
ORIGIN      
        1 ggcgctcgtc gccgccgcgc tggacctcgg cgtcaatttc atcgacaccg ctcccgccta
       61 ccgcaccgag ggcatcctcg gccgcgctct gcgtggcaga cgcgaatccg ccgtggtctc
      121 gacaaaggcg ctcattcacc tcaacacgga gcatccgccg aaaccgtcac ggttcatcac
      181 cggtgccgag ctggaagcgc gcctggacag aagcctggaa gccctgggca cggactacgt
      241 cgacgtctac aaccttcacg gcatcgtcga gcccgagctc gactacgtcg acgccgagct
      301 cgtccccaag ctgctcgacc tgaaagccgc cggaaagatt cgcagcctcg gcatcaccga
      361 gcatttccag gtcgatacgc gacacgagat gctggcccga gcggtgaggt cgggggtgtg
      421 ggacgtcgtc atggtggggt tcaatctgct caacccctgc gcccggcaga acgtgctggc
      481 gccagcccgg gcgatgaacg tcggcgttct caacatgcac gccgtgcgca cctcgctgag
      541 cgacccggac cggctcacgg aagcgctcga gaccgccggg gacgcgggac agctcgatct
      601 gcccccggcc ggcaccgccg ccgcgctcca aagcctggtg agcgaatccg gcgccggctc
      661 gctgaccgaa ctcgcctacc gcttctgtcg ccacgagccc ggcgtgcacg tggtgctgac
      721 gggcacgggc aagctcgacc atctgcgggc gaacatcgcc gccatagaca acgaccccct
      781 gcccgcgccg gtctccgccg gcctggaagg cctcttcgga tccgtcgact gcctgtccgg
      841 cgagtgagcc ggacccggct atatggcaaa cagaccgccg tcgacgagaa actccgcgcc
      901 cgtcgagtag gagctctcgt cactggcgag aaacagcgcc atgctggcaa cctcgtccgc
      961 gtcggcgacc cgaccgaggg gcgtgcctcg cacgagcttc gccggatctc ccgcctcgct
     1021 cagcatctcc gtgtcgatgg ctccgggatg gatagagttc acccgcacgc cggagcgggc
     1081 cagctccacg gcggcggtct tggtcatgcc ccttacggcc cacttggacg cgccataggc
     1141 gaacgagccg gctgccgccg ccatgccggc gtacgaggag atgttgacga tggatcccgc
     1201 ctgcgccttc ttcatctccc ggcccaccgc tcgcagcccc agaaagacac cggtcgcgtt
     1261 cacggccatc acctgctcgt actcgtcgag cgtcgtgtga atgaggcggt tcgcccggta
     1321 gatcccggca ttgttgacga gcacgtcgat tcgcccgtcg acggcgatga tctcgtcaac
     1381 caaggcctgc caaccgctct ccgaggtgac gtcgagcacc cggaactcgc cgccggtctc
     1441 ggccgccagc ttcgagcctt cgtcctccag aacgtcggcg atccagacct tcgcgccttc
     1501 ccgggcgaac gcgcgcgctt ccgccgcgcc ctgaccgcgg gcgccgcccg tgatgagaca
     1561 gaccttgtcc cgaagtcggc tcacttggct tctccttcgt tttcccatct ggccccgccg
     1621 tgcatcggtg cgggactcat tccgcccggg ccagctccgc caccagcgca tcgggcacct
     1681 cgatgagctt ggcacgaagg tactcgccca gggatttagc ctgcttgtcg atccggtgat
     1741 tcgacgcggc tccggtgccc agcaccccgc gaatgtagaa gttcatggcc cgcagattcg
     1801 gcaggtcgta gcgctcgatc tcgtagccgg ccatgtccgg cagcagcttc ttcagctggt
     1861 cgacggtcag gtaacggtgc agaaagccgt acgcgtcatc ggtgcgcgcc cagacgccgc
     1921 agttcgcgtt accccccttg tcacccgaac gggtcccgaa caggcggccg aacggtatcg
     1981 tgaccctgtc cccctcgggc gccggcgcga cgtcgacggg gacctcctga tagtagatct
     2041 cctccaagcc cagacgctgg gtgggcagga cgtcggtggt cgctcctcca acgtgcacgc
     2101 gctccgtcac gtggcggctg tcgacgaggg ccggccagtg aacggttacc ggcgagccgt
     2161 tgaagcccgc cgctccgcgc ccggtgttgc cggggatggc ggcaagcgca agctccgtga
     2221 ccttagcctg cagcaggcgc ccgagcttcg ccgggtccct cgtcgtcacc gtgatcctga
     2281 gcgcagcgtg ggcttcctcg ttggtagcgg gatcttcctt gtcgctccgg atcagctgaa
     2341 cgtccacgtc gtcgaactgc tcgcgcccgc caaggttgtg aaatacctgg tcgacgtaga
     2401 tttcggcctt cttctcgatg tcgagcccgg tgaggagcac ctccaggctc tgcttgcaag
     2461 gacccagcgt gttgaagcag accttgtggg tcgggggcgg gctgctgccc cggcacccgc
     2521 tgacacgaac gcgatcctca cccgtctgct ctatggtcat cgtgtcgaag tgggccacca
     2581 catccggatt gaagtaggcg ggcgttgaga tctcgtagag cagctgagcc gtcaccgtgc
     2641 ccaccgagac caggccgccg gtcccgggat gtttcgtgat cgtgaagctg ccgtcggctt
     2701 cgatctccgc aatggggtag ccgacgtttc gaaagctggg aacctcctcg aagaacgagt
     2761 agtttccgcc gcagcactgg gcgccgcact cgatgatgtg accggcggcc agcgcgccgg
     2821 ccagcgcgtc gtagtcgtta cgggcccagc cgaatttcca cgcggcagga ccgaggacca
     2881 cggccgcgtc cgtcacgcgc gggcagatga cgatgtcggc gccccgatcg agagcttcct
     2941 tgatacccca gccgccgaaa tacacgttgg ccgtgagggc tttctcgtcg gtttccgcga
     3001 gcgccatgcc cgtgtcgagg ttggtgaagg gctcgccttc ggcctgaagc cgctccagat
     3061 cagggagcag atcgtcaccg tcgatgtacg cgacggtggc ctccagcccc agctcggcga
     3121 ggatcttgtc aacctcggcc gccatgctgc gcggattcaa gccgcccgca ttgctgacga
     3181 tcttgattcc ccgctcgatg cagatggcga ctacgtcccg cacctgcttg aggaaggtgc
     3241 cgacgtagcc catgtcctcg ccgcgcgact gaatctgcga gtagaggatc gacatggtca
     3301 gctcagcgag gtagtcgccg gtgagcacgt cgatgggtcc gccttccacc atttcacggg
     3361 cggcttccag ccggtcgccg tagaagccgc tgcagttgcc gatacgtatc acgtcactca
     3421 tcttcgcctt ctcccacggc ccaggccggc ttgcgcttct cgcgaaacgc cgccatgccc
     3481 tcggctgcct cgtcggatcg gaacatgcgg gagctccact cggcggcctc gcggaaaccg
     3541 tcttccacgg tcagccgggg cacgcggcgc acgagcttct tgcactcgac catcgcgttc
     3601 ggaccgccga gattgatcat gtcgatctct tcctgcaccg cgtcgaccag ctcgtctgcc
     3661 gggaccgctc gatgggcgaa gccgatctcc accgcgcggc gccccgagaa ccgctcgccg
     3721 gtgagtatca gcttcatccc gtggtgcacg ccgagcttgg gcaggcagac cacggagatg
     3781 actgccggga tgacgccgat gcggacctcg ctgaagctga actgaacgtc ttccgcggtc
     3841 accacgatat cggcggcccc gatgagaccg aggcccccgg caaacgccgc gccgttcacg
     3901 gcggcaatga cgggcttcgg gctgtcgagg atggcgctga gaacgtccgc atacggcacc
     3961 gcgcgaccgc catcgatggc ctgaccgggc ggctccttca gatccgctcc agcacagaag
     4021 gccgggccgg tcccggtaat gacaatcgaa cgcacggcgg cgtcgtcatt ggcgaccaga
     4081 aggtgatcgt agagctcgtt caccagggga gccgagagcg cgttgcggtt cgcgggccgg
     4141 ttcagggtga tccaggccgc ccccttgcgc gtctcgtaaa gtgttgcttg ggtgccggcc
     4201 actgggttca gccctcctta ttccgccagg gtcacgagca gctgcccgtt ttccacctga
     4261 tcgcccgcgt taacgtgaac cttcgccacg accccatccc gcggcgccgt gatgcgatgt
     4321 tccatcttca ttgcttccag gaccagcagg agctgtcctt tgaccacttc cgtcccttcg
     4381 gctacctggg tctccagcac gcgcccgggc atcggcgcga gcatgcccga gcccagatca
     4441 ccgccgccgg ctagcggata gcgcgggagc tccaccagct ccacgtttcc cgagctgccg
     4501 tgaaccatcc aggtatctcc gagccgccca accaggaatc tcaggcgctg gccgtttatc
     4561 tccagatccg tttctgcgcc gccggctccg tgaacgcgaa ccagcttctc cgattcgtcg
     4621 gccacgaacc ggaacatccc gtcacgacgc gcccggtagc gtagctgcac ttccccggct
     4681 cctgccgagt agcccaccgt ctccatgggc atggtggagt tccgccagcc gccgggtatg
     4741 gttcccatga cccgcgcgct cttccggcgg gcgtctcgcc cttcgatggc ggcggcaatg
     4801 gccgcgttca ccagctcatc ccggccgatg catctgacca ctgccggccg aacgcgctcg
     4861 atgaaatcgg tggtggtgtc gccggccaga aactccggcg tccgcagcgt cgccaccagg
     4921 aaatcccggt tggtggtgag cccctggatg cgcgtcgtct ccagcactcg cgccagccgg
     4981 gtcgccgctt cccggcgtgt cggcgcgtgc acgatgacct ttgcgatcat gggatcgaac
     5041 tcggtgccga tgaccgagcc ggactccacc ccggaatcga aacgggcgct gcccgcccgt
     5101 gacggcgccc aggcgacgac ggtgcccggt gacggcagaa agtctcggcc ggggtcttcc
     5161 gcgtaaagcc gcgcctggat ggcatggccg gagatggcga gctcttcctg ggtgaaggaa
     5221 agccgctccc cctcggcaac acgaatctgc tcccgcacca gatcccaacc gatcacggcc
     5281 tccgtgaccg ggtgctccac ctgaagccgc gcgttcacct ccagaaagta gaactcgtcg
     5341 ccgctcagca gaaactccac cgttccggca gacgtgtagc cgagcttctt tgcggccgcc
     5401 accgcggccg cacccatgcg ggctcgaaga tccggcgtga ccgccggcga gggcgcttcc
     5461 tcgatgatct tctggtggcg ccgctggatg gagcactcgc gctcgaagca gtgcagcacg
     5521 tttccgtgac catcaccgag aatctgcacc tctacgtgtc gcgagctctc cagccacttc
     5581 tcgaaaaaca ccgtatcgtc accgaaggag gacgcggctt cgcgcttcgc gcccagcacc
     5641 gcgtcggcca gcccgccctc gtcctcgacg accctcatgc cccgcccgcc gccgcccgcg
     5701 gaagccttca ccagcaccgg gtagccaatc tgcgatgcgg cttccgcaag atccgtcgcc
     5761 tcggtgatct ccaccgcagg cagggtaggc acgccggctt ctaccatgag ccgctttgcc
     5821 gacagcttgt cccccatgag cccgatcacg cgaggggccg gcccgatcca ccgcaccccc
     5881 tcgtccacca cggcctgggc gaaacgctcg ttctccgaga gaaatccata cccggggtgc
     5941 acggcgtcag cgccgctctg gcgacaggct tcgatgacct ttgggatgtc gagataggtc
     6001 tcggtactgg aacggccccc gagcgcaatg gccgtatccg cttcgcgcac gtagggcgca
     6061 tccgcgtccg catccgcgta cacggcaacg gtgccgattc cgagctcgtg ggccgtgcgg
     6121 atgatgcgac gggcgatctc gccgcgattg gcgacgagga tccgcgagat cggcctgctc
     6181 acatccgcca cgtgccgaac tccttggtcc cttccacccg attgttgaag caggcagaca
     6241 gggcaatcgc gaccacggtg cgagtgtccc gggggtcgat catgccgtcg tccgagaccc
     6301 gggaggttgc gtacagcccc ttggagcgct cttcggccca atgctcggcg acggccgccc
     6361 ggctcgcatc ggcctcctcg tcgaattcga tgccacgccg ggccgccgcc gcgcggccaa
     6421 cgatgctcat cacgcccgcc atctgcttcg ggcccatgac ggcgatcttg gcgaacggcc
     6481 agatgaaggt gaaacggggc ccgaacgcgc gcccgctcat gccgtagttg cccgctccgt
     6541 acgaggcgcc aacgatgacg gtgatgtgcg gaaccgtgga gttggcaacg gcgttgatca
     6601 tcttcgaacc gttcttggtg atgccgccct gctcgaaatc cgtgcccacg atgaagccgg
     6661 tgatgttgtg gaggaacagc agcggcacgt cgatctggtt gcacagctga atgaactggg
     6721 ccgccttctc cgaagattcc gagaagagcg cgccgttgtt gcccaggatg ccgacggggt
     6781 agccatgaat gctggcccag ccacacacca gggtgggccc gtacaagggc ttgaactcct
     6841 cgaagtagga ggcgtcgacg atgcgggcga tgacttcgcg catgtcgaag ggcgagcgca
     6901 gatccttgga gaccatgccc agcagatcct cgggatcgtg aaggggctcg gcgaagtcgg
     6961 ctctcggctc cgggccgagc ttgcgccaat tgagatgctt gaccacttcc cgacacatcc
     7021 ggatgccgtc
//
LOCUS       DBNHKV010000298         5496 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_467452, whole genome shotgun sequence.
ACCESSION   DBNHKV010000298 DBNHKV010000000
VERSION     DBNHKV010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_467452"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="AC16PP_09345"
     CDS             complement(<1..1529)
                     /locus_tag="AC16PP_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR6481874.1"
                     /translation="MTSRRPTSDKPVVGGGVKKVLYTVETVRRMGLARAAKALSANNA
                     CKACGLGMGGQRGGMTNELGDFPAVCNKSVQAQSTDIQPAIPLEAFSHTLSELRELDE
                     RELASLGRLGQPLFKEAGAERFRCVDWSWALARAAARLGATDPARTFFYSSGRSSNEA
                     GFIFQLLARAYGTNNVNNCSYYCHQATNVALDASIGTGTSTVELADLTGCDLIFLIGA
                     NPASNHPRLLHMLKSCRDRGGRVVVINPAKEPGLVRFAVPKSARSMLSGGSWIASEYL
                     QPRIGSDVALLKGIAKAVVEQGAAASDFIDRHTEGFEAYAADLGATRWSDVEAATGIA
                     RAEIERVAAAYAGSDNAVFAWGMGLTHHLNGVDNVESVANLALLRGMVGKRYAGLLPL
                     RGHSNVQGIGTIGVKPVLPEEVLARMESAFGVQFPDKPGLDTMAALQAADRGEIDAAL
                     VMGGNLYGATPQSRWAEGALARIGFKMFMTTTLNRGHLYGLGEGEALVLPVTARDEEW
                     EP"
     gene            1734..2069
                     /locus_tag="AC16PP_09350"
     CDS             1734..2069
                     /locus_tag="AC16PP_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HIR6481875.1"
                     /translation="MSRTLFAAFALVIAGAASAGTNDYRFTYTNADFADVESVKEMYA
                     RIAKTAREYCPGYFPTRNLSAMSRCRAEVTADIVASIHNPALTALSKGEDAQVRVALD
                     NADRTSGRG"
     gene            2207..2776
                     /locus_tag="AC16PP_09355"
     CDS             2207..2776
                     /locus_tag="AC16PP_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR6481876.1"
                     /translation="MRVATHLQWTNRRAARFACYLLCSIFVALPLHADDDAGAPTQLA
                     PDFTLPNLLDRDQSIRLSDYRGKTVYLDFWSAWCIKCRESLPLLTELNEQLGGGEFAV
                     VTVNIDMNPGDGRRLLEQLAVNYPVASDVSGSTADRFGAVTLPATYLIDGDGNVRPPL
                     PTMNKQNFSAIKALLAEMIERDKRGTFGT"
     gene            2878..4263
                     /locus_tag="AC16PP_09360"
     CDS             2878..4263
                     /locus_tag="AC16PP_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012344.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6481877.1"
                     /translation="MALAIVLVILIIGSVAFHLWSPWYLTELASNWDTIDYTIDITFW
                     VTGFVFVAVNAFMAWAVFRYRYSKDRRADYEPENKKLEWWLTGLTTIGVVAMLAPGLF
                     VWGEFVEVPEDAWEMEVVGQQWHWTYRFPGEDGKLGTSKVTLITQDNPLGVNPEDPNG
                     LDDKIIYDPVVHIPSGRPMKALLRAKDVLHNFTVPQFRVKMDLVPGLVSYVWLIPEKN
                     GTYDILCEELCGVGHYTMRGRITVQDQADFDAWLAQQPTFAETQARGHGDPVAGQAAY
                     AVCAACHGMQGEGNPMMNGPKLTGQSTWYLRRQIGAYKRGHRGTHPDDVYGRQMAPMV
                     ATLVNQQAVDNVVAYIRSLPDVPAQHTVTGDLEHGAELYEGCVLCHGADGMGKHATNA
                     PRYVGMSDWYLVRQLKNFQTGIRGSHPEDKYGEQMGFMSKQLYGDESINDVVAYINSL
                     PPGAAVTTASR"
     gene            4348..>5496
                     /locus_tag="AC16PP_09365"
     CDS             4348..>5496
                     /locus_tag="AC16PP_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019690290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR6481878.1"
                     /translation="MAYVAIADRDEHLHDPDTFITKYIWSQDHKVIAIQYGLTAIFVG
                     LVALVLSGLMRLQLGFPDQFSFIDPGAYYQYITMHGMIMVIYLLTALFLGGFGNYLIP
                     LMLGARDMVFPYMNMLSYWVYLLSVIVLMLSFFAPDGPTGAGWTLYPPQAILPGTPGV
                     DMGIVLMLGSLALFIVAFTMGGLNYITTALQARTRGMTLMRMPLSVWGIVMATVLGLL
                     AFPALLVSAIMMLLDRLLGTSFFMPAVVSLGDQLSYEGGSPILFQHLFWFFGHPEVYI
                     VALPAFGIVSDLISVHARKNIFGYRMMVWAIVAIGVLSFIVWAHHMYVSGMHPYFGFF
                     FATTTLIIAVPTALKVYNWTLTLWRGNILFTVPMLFSLAFVVTFVVGGL"
BASE COUNT         1047 a         1745 c         1666 g         1038 t
ORIGIN      
        1 ggctcccact cctcgtcccg agcggtgacc ggcagaacga gcgcttcacc ctcgccgagc
       61 ccgtagagat ggccccgatt gagagtggtg gtcatgaaca tcttgaagcc gatcctggcg
      121 agcgcgcctt ctgcccagcg tgactgcggc gtggcgccat ataggttgcc ccccatgacc
      181 agcgcggcgt caatctcgcc ccgatcggca gcctgaagag cagccatggt gtccagaccc
      241 ggcttgtccg gaaactgaac gccaaacgcc gactccattc gagccagaac ttcctccggg
      301 agcacgggtt tgacgccgat ggtgccgatt ccctgaacgt tgctgtggcc gcgcaggggc
      361 agcaggccgg cgtaccgctt gcctaccatg ccgcgcagaa gcgcgaggtt ggcaacggac
      421 tccacgttgt cgacgccgtt gagatggtgg gtcagcccca tcccccaggc gaaaaccgcg
      481 ttgtcagagc cggcatatgc ggccgctacc cgctcgatct ccgcccgcgc tatccccgtg
      541 gccgcttcga cgtcggacca gcgagtggcg ccaaggtcgg cagcataagc ttcgaagcct
      601 tcggtgtggc ggtcaatgaa gtcgctggcc gccgctccct gctccaccac ggcctttgca
      661 atgcccttga gcagcgccac gtcggagccg atgcgcggct gcagatactc actggcaatc
      721 caagaccctc ctgagagcat cgacctggca ctcttgggca cggcaaaccg caccagcccc
      781 ggttcctttg caggattgat gaccaccacg cgaccaccac ggtctcgaca gcttttgagc
      841 atgtgcagca gacgcgggtg attcgacgcg gggttggccc cgatgagaaa gatcaggtcg
      901 cagcccgtca ggtccgcgag ttccacagtt gaggtaccag tgccgatgga ggcatccagc
      961 gcgacgttgg tggcctgatg acagtaatag gagcagttgt tgacgttgtt ggtgccgtac
     1021 gccctcgcca gcagctgaaa gatgaagcct gcctcgttgg acgatcggcc ggagctgtag
     1081 aagaacgtcc gggcggggtc ggtggcgccc aggcgggcgg ccgcccgcgc caaggcccag
     1141 gaccagtcga cgcagcggaa acgctccgct cccgcttcct tgaacagcgg ctgaccgagc
     1201 cgaccgaggc tcgccagttc ccgctcgtcg agttcacgaa gctcgctaag cgtgtgcgag
     1261 aacgcttcga ggggaatcgc cggctggata tcggtggatt gcgcctgaac gctcttgttg
     1321 cacaccgccg ggaagtcgcc cagctcgttg gtcatgcccc cacgctgacc gcccatcccg
     1381 agcccgcagg ctttacacgc gttgttcgcg ctgagcgctt tcgccgcgcg ggcgagaccc
     1441 atacggcgaa ccgtctccac ggtgtagagc acctttttta ccccgccgcc gacgacgggc
     1501 ttgtccgagg tgggccttct cgatgtcacg acaacgttcc ggaaaccgcg aggaggacag
     1561 tatagagctt tcgagcgagt ctgcgtaacg ctggtaaccc attgtttttg atgagatcaa
     1621 aaaaaatttt cggcgaaatt gcctgaggga cgcaacggtt tcggacgctg ctgcatctat
     1681 agatacgtag gccatcgaaa cgtaccaagc caacgggaaa aggattgaaa atcatgagta
     1741 gaactctctt tgccgcattc gccctcgtga tcgctggggc cgcttcggcc ggaaccaacg
     1801 attaccggtt cacatacacc aacgccgact tcgccgacgt cgaaagcgtc aaggagatgt
     1861 acgcgagaat cgccaagacc gcgcgggagt attgccccgg atatttcccc acacgcaatc
     1921 tgagcgcgat gtcgcgttgc cgggctgagg tgaccgcgga catcgtcgcc tccatccaca
     1981 atccggccct gaccgccctg agcaagggcg aggatgcgca agtccgcgtc gccctcgata
     2041 acgcggaccg gacgagcggc cgcggttgat ccggcgcgaa gccaccacgc cgggctgagt
     2101 ttcagcccac aacgacaggg cgcttcatgc gtcctgtcgt cttgctctac tctccggttc
     2161 ctgagagact acgaaagaga tggggagcct gagtgatgct cagagcgtga gagttgcaac
     2221 gcatttacag tggacaaacc gccgcgcggc gcggttcgcc tgctacctcc tctgctccat
     2281 ctttgtcgcc ctgccgctgc atgcggacga cgacgccggt gcaccgacac agctcgcgcc
     2341 cgacttcaca ctaccgaacc tgctcgatcg cgaccaatcc atccggctct ccgattaccg
     2401 aggcaaaacg gtctatctcg acttctggtc ggcctggtgc atcaagtgcc gggaatcgct
     2461 gcccctgctc accgagctca atgagcagct tggcggtggg gaatttgccg tcgtcaccgt
     2521 gaatatcgac atgaacccgg gcgacgggag gcggctgctg gagcagttgg cggtcaacta
     2581 ccctgttgca agtgacgtga gcggctccac ggcggaccgc ttcggagcgg tcacgctgcc
     2641 ggccacctac ctgatcgacg gtgacggaaa tgtgcgcccg ccgctgccga cgatgaacaa
     2701 acagaatttc tctgccatca aagcgctctt ggcagaaatg atcgaacgag acaaaagagg
     2761 tacgttcggc acgtaggaca ttccagttgt ggccggtccc tcggggaggg acattgacgg
     2821 gccgcaagtg ataatcgacg caataatata gacgcaatat agtagagggg ctattagatg
     2881 gcgttggcga tagtactcgt aatcctgatc attggatcgg tcgcgttcca tctgtggagc
     2941 ccgtggtatc tgacggagct cgcctcgaac tgggacacca tcgactacac catcgacatc
     3001 accttctggg tgaccggctt cgtcttcgtc gccgtcaacg ccttcatggc gtgggcggtc
     3061 ttccgatacc ggtacagcaa ggatcgtcgc gccgactacg agcccgaaaa caagaagctc
     3121 gagtggtggc tcaccgggct gacgacgatc ggggtagttg cgatgctggc cccggggctg
     3181 ttcgtgtggg gcgagttcgt cgaggttccg gaggatgcat gggagatgga ggtcgtcggt
     3241 cagcaatggc actggaccta ccgcttcccg ggagaggacg gcaagctggg cacctccaag
     3301 gtgacgctga tcacgcagga caatccgctc ggcgtgaacc cagaggaccc caacggcctg
     3361 gatgacaaga tcatctacga cccggtggtg cacatcccca gcgggcggcc catgaaagcg
     3421 ctgctgcgcg cgaaggacgt tctgcacaac ttcaccgttc cccagttccg ggtgaagatg
     3481 gacctggtac ccggactcgt ctcgtatgtc tggttgatcc ctgagaagaa cggcacctac
     3541 gacattctct gcgaggagct ttgcggcgtc ggccactaca ccatgcgcgg ccgcatcacc
     3601 gtgcaggacc aggccgactt cgacgcctgg ctggcgcaac agcctacgtt tgcggaaacc
     3661 caggcgcgtg gccatggcga tcccgtggcc ggccaggctg cctacgcggt ctgcgccgcc
     3721 tgtcacggca tgcagggcga aggcaatccc atgatgaacg gcccgaagct gaccggccag
     3781 agcacgtggt atctgcgccg gcagatcggc gcctacaaac gcggccaccg cgggacccat
     3841 ccggacgacg tctatggacg ccagatggcg cccatggtcg cgacgctcgt gaatcagcag
     3901 gccgtcgaca acgtggtggc ctacatccgg agcctgcccg acgtgccggc gcaacatacc
     3961 gtcaccggcg acctggagca cggcgcggaa ctctatgaag gctgcgtgct gtgccacggg
     4021 gccgacggga tgggcaagca cgccaccaac gctccgcgct acgtggggat gagcgactgg
     4081 tatctggtca ggcagttgaa gaacttccag accggcatcc gaggctccca tccggaagac
     4141 aagtacggcg agcagatggg cttcatgtcc aagcagctgt acggcgacga atcgatcaac
     4201 gatgtggtcg cgtacatcaa ttccctgccc cctggcgcag cggtgaccac cgcctcacgg
     4261 tgatcatgat cgcagggtag gcaggacaga cgacgagatg agcaaaatac tgaagagcaa
     4321 ccagcacaac ggcagccgga ggatctgatg gcatatgtcg caattgcgga tcgtgacgag
     4381 caccttcacg atccggatac attcatcacc aagtacatct ggagccagga ccacaaggtc
     4441 atcgcgatcc agtacgggct caccgccatc ttcgtggggc tggtggctct ggtgctctcc
     4501 ggcctgatgc ggctgcagct gggtttcccg gaccagttct cgttcatcga tccgggcgcc
     4561 tactatcagt acatcaccat gcacggcatg atcatggtga tctatctgct gaccgccctt
     4621 ttcctgggcg gcttcgggaa ctacctgatc ccgctgatgc tcggcgcacg ggacatggtt
     4681 ttcccatata tgaacatgct gagctactgg gtatacctgc tgtccgtcat cgtgctgatg
     4741 ctcagcttct tcgcccccga cgggccgacc ggagcaggat ggacgcttta cccgccgcag
     4801 gccatcctgc cgggcacgcc gggcgtggac atgggcatcg tgctgatgct cggctccctc
     4861 gcgctgttca tcgtggcctt cacaatgggt ggtctgaact acatcaccac ggctctgcag
     4921 gcacgcaccc gcggcatgac gctcatgcgg atgccgctgt cggtttgggg aatcgtgatg
     4981 gccaccgtgc ttggtctgct cgccttccct gctctcctcg tgagcgccat catgatgctg
     5041 ctggaccgtc tgctgggcac cagcttcttc atgccggccg tcgtctctct cggcgaccag
     5101 ctcagctacg aaggcggcag cccgatcctg ttccagcatc ttttctggtt cttcggacac
     5161 cctgaggtct acatcgtcgc gctgccggcg ttcggcatcg tgtccgacct catcagcgtg
     5221 cacgcccgca agaacatctt cggctaccgg atgatggtgt gggcgatcgt tgccatcggc
     5281 gtgttgagct tcatcgtctg ggcccaccac atgtacgtga gcggcatgca cccttacttc
     5341 ggcttcttct tcgccaccac gaccctcatc atcgcggtgc cgacggcact caaggtgtac
     5401 aactggaccc tcacgctctg gcgcggaaac atcctgttca ccgttccgat gctgttctcg
     5461 ctcgccttcg tcgtgacgtt cgtcgtcggc ggtctg
//
LOCUS       DBNHKV010000299         5036 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_41329, whole genome shotgun sequence.
ACCESSION   DBNHKV010000299 DBNHKV010000000
VERSION     DBNHKV010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_41329"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1636)
                     /gene="glnD"
                     /locus_tag="AC16PP_09370"
     CDS             complement(<1..1636)
                     /gene="glnD"
                     /locus_tag="AC16PP_09370"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HIR6481879.1"
                     /translation="MSTTERAARSPGSDGNEGSRRFGNADPFLEELKVRLAQADERMA
                     QRYWDRADVDELIAERTTFFDGLLREVWDHALSEDAQKGLALFAVGGYGRREQFPYSD
                     IDLLIVGKRPASHREAISRFLQVLYDLNLEVGHSVRTVRDCKKEAQKDITVATAIFER
                     RFLFGSDALRRRLDRVLSSSRLWPAEAFFRAKRDEQAHRHRQYDNVEYGLEPNVKSSP
                     GGLRDLQTALWVCQRKFGTTDPEALEALGMLTPLEREWLTEGRRYLWWVRFGLHLIAG
                     RKDDHLQFEHQRALAQRLGFADTNARLGVERFMHLYYRHVLELRELNDILLQHFEEAI
                     LSSRGRPRIEPINERFQIHNDYIEATDPTVFERSPRAILEIFVIMANRRDISGVRAQT
                     IRLIRDSLHLIDDSFRADPENAALFMDLLKAPYTLVSQLTRMRRYGVLARYLPEFGRV
                     VGQMQHDLFHIYTVDAHTMMVIGNMRRFRYRASEERFPVACHCVKEIPKPELLYVSGL
                     YHDIGKGRGGDHSELGAGDVTEFCRRHGLNEADTDLV"
     gene            complement(1656..2447)
                     /gene="map"
                     /locus_tag="AC16PP_09375"
     CDS             complement(1656..2447)
                     /gene="map"
                     /locus_tag="AC16PP_09375"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR6481880.1"
                     /translation="MVAKLENEDDIARMRNAGRLAAEVLEMIGEHVRPGASTEDLNGI
                     CHRYIVEEIDCIPAPLNYSNGPGQPAFPKSICTSVNHVVCHGIPAAKKVLKSGDIVNI
                     DVTVIKDGFHGDTSKMFMVGKPSVLAERLVRVTQECLYRGIDAVRAGATLGDIGHAIQ
                     SHAEAQHFSVVREFCGHGIGRVFHDEPQVVHYGRPNTGMRLEEGMTFTIEPMINAGRR
                     HVKILPDHWTVVTKDHKLSAQWEHTILVTATGAEVLTRRSDESLP"
     gene            2692..3328
                     /gene="rpsB"
                     /locus_tag="AC16PP_09380"
                     /pseudo
     CDS             2692..3328
                     /gene="rpsB"
                     /locus_tag="AC16PP_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
     gene            3661..4530
                     /gene="tsf"
                     /locus_tag="AC16PP_09385"
     CDS             3661..4530
                     /gene="tsf"
                     /locus_tag="AC16PP_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR6481881.1"
                     /translation="MAITAAMVKELRERTGLGMMDCKAALTEAGGDMELAIEELRKRS
                     ALKAAKKAGRTTADGLLGIKVAEDGSRGAIVEVNIETDFAAKNQKFVDFVGEVLAAVY
                     DGREDDVAKLVAGELEAKREALVQEIGENITVRRAALLGAGDGFVTFYLHGDNRKGTL
                     VELSSPNEELGRDLAMHVTAIAPMVVESSGVPADVLAKEREIYASQAEASGKPAEIVE
                     KMVEGRVRKFLAEVSLVDQPFVKDPDVKIGKLLSDAGVECRGFVRFEVGEGLEVEQTD
                     FAAEVAAQVSGAE"
     gene            4567..>5036
                     /locus_tag="AC16PP_09390"
     CDS             4567..>5036
                     /locus_tag="AC16PP_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR6481882.1"
                     /translation="MQRLLLKLSGEALGGDVGFGIDPVVVRRLGEEISEVIAAGAEVA
                     LVLGGGNLFRGAALAEAGMDRITGDQMGMLATVMNGLAVRDLMRSGGVPAEVLSAIEI
                     PGVARKFTRDAAQELLRNGQVAIFVAGTGNPYFTTDTAACLRGIEIGADVVLKAT"
BASE COUNT          905 a         1545 c         1688 g          898 t
ORIGIN      
        1 ccacaagatc ggtatctgct tcgttcagcc cgtggcggcg acagaactcg gtgacgtcgc
       61 cggcgccgag ctccgagtgg tcgccgcccc gtcctttgcc gatgtcgtgg tagaggcccg
      121 acacgtacag gagctccggc ttgggtattt ccttcacgca gtgacaggcg accggaaagc
      181 gctcttcgct cgcgcgatag cggaaccggc gcatgttgcc gatcaccatc atcgtgtgcg
      241 cgtccacggt gtagatgtgg aagaggtcgt gctgcatttg cccgactacg cgtccgaact
      301 ccggaaggta gcgcgccagc accccgtacc ggcgcatcct cgtcagctgc gagaccagcg
      361 tgtacggcgc tttgagcaga tccatgaaca gcgccgcgtt ctccgggtcg gcgcggaacg
      421 agtcgtcgat gaggtgaagg ctgtcccgga tcaggcggat ggtttgcgcg cgcacccctg
      481 agatgtcccg ccgattggcc atgatgacga agatttccag gatggctcgg ggcgatcgct
      541 cgaacaccgt ggggtcggtg gcttcgatgt agtcgttgtg aatctgaaaa cgctcgttga
      601 tgggctcgat gcgcggccgg ccgcggctgc tcaggatcgc ttcctcgaag tgctggagca
      661 gtatgtcgtt gagctcgcga agctcgagca cgtgacggta gtacaggtgc atgaaccgct
      721 ctacgcccag gcgggcgttc gtgtcggcaa atcccagccg ctgcgcgaga gcgcgctggt
      781 gctcgaactg caggtggtcg tccttgcgcc cggcgatcag atgaaggcca aaccgcaccc
      841 accacagata gcggcggcct tccgtcagcc actcgcgttc caggggggtg agcatgccaa
      901 gcgcctccag agcttcagga tcggtggtgc cgaacttgcg ctggcacacc cacagcgccg
      961 tctgcagatc cctcagcccg ccgggcgacg atttgacgtt gggttcgaga ccgtactcga
     1021 cgttgtcgta ctgccggtgg cggtgcgcct gctcgtcgcg cttggcgcgg aagaaagcct
     1081 ccgccggcca caggcgagat gacgacagca cccgatcgag ccggcgccgc agcgcgtcgg
     1141 acccgaacag aaaacgccgc tcgaagatgg ccgttgccac ggtgatgtcc ttctgcgcct
     1201 ctttcttgca gtcccgaacc gtgcgaacgc tgtgcccgac ctccaggttc aggtcgtaga
     1261 gcacctgaag aaaccggctg atggcctcgc ggtggctggc cggccgcttg ccgacgatga
     1321 ggaggtcgat gtccgagtag gggaactgct ctctgcggcc gtagccgccc acggcgaaga
     1381 gggcgagccc cttttgcgca tcttcgctca gggcgtggtc ccacacctcg cgcagcaggc
     1441 cgtcgaagaa cgtcgtgcgc tctgcgatca gctcatcgac gtcggcacgg tcccagtatc
     1501 tttgggccat acgttcatcg gcctgagcaa gacgaacctt cagctcttcg agaaagggat
     1561 ccgcgttgcc gaaacggcgg ctgccctcgt tcccgtcgct gcccggagat cgggccgctc
     1621 tctctgtcgt gctcatgacg acgtggcctt cgtgatcaag gcaacgattc gtcgctgcga
     1681 cgggtgagca cctcggcacc cgttgcggtg actaagatgg tgtgttccca ctgcgcagaa
     1741 agcttgtggt ccttggttac cacggtccag tgatcgggca ggatcttcac gtgacgtctg
     1801 cccgcattga tcatgggttc gatggtgaag gtcatgcctt cctccaggcg cattcccgtg
     1861 ttcgggcggc catagtgcac gacctggggt tcgtcgtgaa aaacccggcc gatcccgtgg
     1921 ccacagaact cgcgcacgac ggagaagtgc tgagcttcag catggctctg gatcgcgtga
     1981 ccgatatcgc ccagcgtcgc gcccgcgcgc accgcgtcga tgccccggta caggcactcc
     2041 tgggtcacgc ggaccagccg ctctgccaag acgctcggct ttcccaccat gaacatcttg
     2101 ctggtgtcgc cgtggaagcc gtccttgatg acagtgacgt cgatgttgac gatgtctccg
     2161 ctcttgagca ccttcttcgc tgccggaatc ccgtgacaga cgacgtggtt gaccgacgtg
     2221 cagatggatt tcggaaacgc gggctgaccg ggcccgttgg agtagttcag cggcgccggg
     2281 atacagtcga tctcctccac gatgtagcga tgacagatcc cgttgagatc ctcggtggag
     2341 gccccggggc gaacgtgctc gccgatcatc tccagcacct cggcggcaag ccgccccgcg
     2401 ttgcgcattc ttgcgatgtc gtcctcgttc tcgagcttgg cgaccatgaa tctacgtctg
     2461 gcttgggctt gtttaagcgg tggggcgatt atgttataaa gcgcgccgcc tgcgggcaac
     2521 gcaagccgcc caacttcgga agaaatatct gcgggagggg ggcggcgggc tccaggcgac
     2581 taaacgtcac tttgacacaa atgactcacg tcgtccgaca cgtgtctccg ggtgcccgtt
     2641 cgatcgggtt gaggcatggg gatggcggag gcgcaacccg gagtacagga tatgaatatc
     2701 agcatgcgtg acatgttggc cgctggcgtg catttcggcc atcagacccg tttctggaac
     2761 cccaagatgg ctcccttcat cttcgggtcc cgtaacaaga tccacatcat caatctggag
     2821 agaaccgtcc cggcgttcaa cgaggcgctg acggagctcc gaaacctcgc cagccgtcgc
     2881 aacaagatcc ttttcgttgg caccaagcgt gcggccgcca aggtgatccg ggagcaggcc
     2941 gagagggtag gcatgcccta cgtcgaccag cgctggctcg gcggcatgct gaccaactac
     3001 aagaccatcc ggcaatccat caagcggctg caggagctgg aatctcaggc cgttgacggc
     3061 accttcgagc tgctcaccaa gcgcgaggcg ctgcagcgcc ggcgcctgat ggagaagctg
     3121 gagcggagcc tcggtggcat caaggagatg gccgggctgc cggacgcgat cttcgtcatc
     3181 gacgtgatgc gcgagcatat cgccatcagc gaagcgaaca agctcgggat ccccgtcttc
     3241 ggcgtcgtgg acaccaacag cgaccccgac ggcgtcgact gggtcatccc cggaaatgac
     3301 gatgccatcc gggcgatccg gctctacgtc accgcggtgg cggatgccat tcaagaaggc
     3361 gtcgacaacg cggccggtgc cgtacgcccc ggcgaattcg tcgaggtcga ggcggagcag
     3421 gtggaggaag cgcctgagac tgcgcccgcg actgcgtccg cgactgcgtc cgcgactgct
     3481 cctgcggctg ccgcggctcc ctcggctccc gcggcggagc ctgaggctgc cacgaccgcc
     3541 gaagctcagg cgggagacgt tgcagccgag ccggagaccg gcgctgccga gactggttcg
     3601 gacgaagctg cagagtcggc agaggccgat gccgagaaca aaccgacgga ggcttgagta
     3661 atggcgatca ccgcagcaat ggtcaaggaa ctcagggagc gtaccgggct tggcatgatg
     3721 gactgcaaag ccgcgctgac cgaggccggg ggagacatgg agctggccat cgaggagctg
     3781 cgtaagcgca gcgccttgaa ggctgccaag aaagccggcc gcacgacggc ggacgggctt
     3841 ctcggcatca aggttgccga ggatggttcc aggggcgcca tcgtcgaggt caacatcgaa
     3901 accgactttg cggccaaaaa ccagaagttc gttgacttcg tcggcgaggt cttagcggcc
     3961 gtttacgacg gccgcgaaga tgacgtggcc aaactcgtgg ctggcgagct cgaggccaag
     4021 cgcgaagccc tggtccagga aatcggcgag aacatcacgg tgcgccgtgc ggccctgctg
     4081 ggcgcgggtg acgggttcgt cacgttttac cttcatggcg acaaccgcaa ggggacgctc
     4141 gtggagcttt cctcgcccaa cgaggagctc ggtcgcgatc tggcgatgca cgtcacggcc
     4201 atcgccccga tggttgtcga gtcgtcaggc gtgccggcgg acgtgctcgc gaaagaacgg
     4261 gaaatctatg cctcccaggc cgaggccagc ggcaagccgg ccgagatcgt cgaaaagatg
     4321 gtcgaaggac gggtgcgcaa gtttctcgcg gaggtgagcc tggtcgacca gccgttcgtt
     4381 aaggatcccg acgtcaagat cggcaagctg ctgagcgacg ccggggtcga atgccgcggc
     4441 ttcgtgcgct tcgaagtggg cgagggactc gaggtcgagc agaccgattt cgctgccgag
     4501 gtggcggccc aggtcagcgg tgccgaataa acctaagcca gcctcggcgg gccggaggtc
     4561 atagcattgc agagattgct gctgaagctc agcggggagg ctcttggggg cgacgtgggc
     4621 tttggcatcg atcccgtcgt ggtgcgccgg ctgggcgagg aaatttcgga agtcatcgcc
     4681 gccggcgctg aggtcgcgct ggttctcggc gggggcaacc tgtttcgcgg cgccgcgctg
     4741 gcggaggcgg gaatggaccg catcaccggc gatcagatgg gcatgctggc cacggtaatg
     4801 aacggtctgg ccgttcgcga tctcatgcgg tccggcgggg tgccggccga ggtgctctcg
     4861 gccatcgaga tcccgggagt cgctcgcaag ttcacccgtg atgcggcgca ggagctgctc
     4921 cgcaacggac aggtcgccat attcgttgcc gggacgggca acccgtactt caccaccgac
     4981 acggccgcct gcctgcgcgg catcgagatc ggtgccgacg tggttttgaa ggcgac
//
LOCUS       DBNHKV010000300        10639 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_538659, whole genome shotgun sequence.
ACCESSION   DBNHKV010000300 DBNHKV010000000
VERSION     DBNHKV010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10639)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10639)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10639
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_538659"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="AC16PP_09395"
     CDS             <1..696
                     /locus_tag="AC16PP_09395"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR6481883.1"
                     /translation="GRAVATLAGVRGHASLVCSRAVGRCGPVTIGVGDVTRNDGEGHM
                     YYTYMDGPVGRLLLAGDGKRLKLIGFSTGARARGAQQDWERYDEPFRPAKRQLDEYFD
                     GRRRYFDIELAPDVTPFQGKVLEALLEIPYGETRSYGEIARAIGQPRAVRAVGGANGS
                     NPIPIVIPCHRVIGSNGSLTGFGGGLDTKQYLLDLERSHSGLFEGLVGDSQQAPQRPV
                     RAGKGQVVFTVTR"
     gene            complement(706..1251)
                     /locus_tag="AC16PP_09400"
     CDS             complement(706..1251)
                     /locus_tag="AC16PP_09400"
                     /inference="COORDINATES: protein motif:HMM:NF016269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HIR6481884.1"
                     /translation="MRSFLRFLTLCAVCLAPAGAAWSASKVEIDARISAALESLYEIE
                     PAARELAAKASGVLVMPRVYKLGFGLGGEYGEGALLINNQAVQYYRVTAASFGFQLGG
                     QAKSEVLLFMSSEALDGFRNSDGWEAGVDGAITFIEFGVGKDFDTHTLQDPIIGFVYG
                     NKGLMYDLSLEGSKFWRIDKN"
     gene            1481..2065
                     /locus_tag="AC16PP_09405"
     CDS             1481..2065
                     /locus_tag="AC16PP_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HIR6481885.1"
                     /translation="MDTNGLRRDPEGARRRILDSAAEHFSRHGPDGARVDAIAAAARV
                     NKRMLYHYFGDKQALFRAVLDDRATRPGGTGNTSGWDTTTARLLVWAAGLDPVGATPG
                     NLEQWTALARHLGDEQDRGRLRNDIRAGTAAMLYLALAALPALLPAHVRAILGSNDAA
                     MLQGDLRKLLNPPAELHGGSGRPRVRMQPQVRPR"
     gene            complement(2068..3345)
                     /gene="aroA"
                     /locus_tag="AC16PP_09410"
     CDS             complement(2068..3345)
                     /gene="aroA"
                     /locus_tag="AC16PP_09410"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR6481886.1"
                     /translation="MGSLTIQPIQALEGDITLPGSKSISNRALLLAAEAQGQTRLVNL
                     LDSDDTAYMLRALGQLGISITENGGDHLVTGVAGPLVNDERHESLDLGLAGTALRPLT
                     AALTLGRGVFELDGTARMRERPVGHLVEGLRQLGASIRYTGAEGFPPIEVTGTGLLGG
                     RVTMQGNVSSQFLTSLLLAAPLAAGPVEVVVEGEQVSKPYLDITVDMMRRFGATVGHE
                     DHRLFRVEPGGYQSPGRYLIEGDASAASYFLAAGAIRGRGVRVHGIGAGSVQGDMAFV
                     EVLKAMGARVTVDAEWVRVEPGALTGVDLDLNHIPDAAMTLAVLALFAEGSTRIRNIY
                     NWRVKETDRLAAMATELKKLGAEVDECEDSLTVNPPKYMNAASIDTYDDHRMAMCFSL
                     AALGGVAVTINDPDCVSKTFPRYFAEFERLARR"
     gene            complement(3392..3982)
                     /locus_tag="AC16PP_09415"
     CDS             complement(3392..3982)
                     /locus_tag="AC16PP_09415"
                     /inference="COORDINATES: protein motif:HMM:NF018996.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein Syd"
                     /protein_id="HIR6481887.1"
                     /translation="MSVQTALSQFVSHYEKSYPELTEIFDPEWRSPCETSEPYQDDDG
                     IVRVPWSPLARGLKVPEEHDFAGLENALETEIHPDIKAYYGSFWSGGLEAEAPQGHVS
                     LILLWNAEDASRLIENLIGHALAKRRARAPLSVFFACTDQDSELFLSVENTTGEVLIE
                     KPGHKPVDVVAGSLASFIDSLVPAPPELHPERSLLR"
     gene            complement(4021..4509)
                     /locus_tag="AC16PP_09420"
     CDS             complement(4021..4509)
                     /locus_tag="AC16PP_09420"
                     /inference="COORDINATES: protein motif:HMM:NF025173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HIR6481888.1"
                     /translation="MPRSKWLVLLLVLSLGANLAMAGFLAGRATGDGGGPRLLDPNLR
                     VTRMLLQQLPEERREALRPLVRDQVRSIRPSVGGIREAQRDIEAALVAEPFHPEKLEE
                     ALGAFRTHLSRSQETSHAAFVGLARSLSPEERRLLGKALAGSGRHRPHGHPPPGMPAV
                     RP"
     gene            complement(4499..4915)
                     /locus_tag="AC16PP_09425"
     CDS             complement(4499..4915)
                     /locus_tag="AC16PP_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481889.1"
                     /translation="MNIDDFEQGLDRWGSDLDSWPDPERDAASMLVERSERARALMVQ
                     GQALDGWLDEGRTHRAPMQLKQKILDQLPRPDIWQRAADWFASAFWPPALTGACALLL
                     GFATGTLLPAANEEALIDDVAGLAFTETYLEFEDAP"
     gene            complement(4941..5450)
                     /locus_tag="AC16PP_09430"
     CDS             complement(4941..5450)
                     /locus_tag="AC16PP_09430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6481890.1"
                     /translation="MVLIAKQDHEAFSHLLTRHLDSIHRYLVRLAGSTADADELAQET
                     FLRVWLNARTFEAGRVAVTTWMHRIAHNVCVDELRRRRRTTSLDEAPEPGVDERGVHQ
                     ATERLEHLQAALGSLPEAQRSAIVLSRIQGFSNQDAARIMNVSVHALESLLARARRNL
                     RLRLADLSE"
     gene            complement(5494..6096)
                     /locus_tag="AC16PP_09435"
     CDS             complement(5494..6096)
                     /locus_tag="AC16PP_09435"
                     /inference="COORDINATES: protein motif:HMM:NF024890.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HIR6481891.1"
                     /translation="MTMKTTITLALLALTGLATGTSWSHHHDRDFPISIAEAEARAAE
                     AFGEIDANGDGKITSEEFANAERPMPKGRHHAHSGTHRAERGAHHRPGHHGDGPPPDQ
                     AALDAALFDALDADGDGKLSRGEFSHEKMHENHQRLRREETFARLDSNDDGVLTNDEL
                     LDRRLERLKKVDADGDGNVTRDEMKAAMREHHGGDRGPGG"
     gene            complement(6150..6653)
                     /gene="greB"
                     /locus_tag="AC16PP_09440"
     CDS             complement(6150..6653)
                     /gene="greB"
                     /locus_tag="AC16PP_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HIR6481892.1"
                     /translation="MSRYRPPRPRSSPYITPAGQERLAAELKELWKVKRPEVTQKVAE
                     AAALGDRSENAEYIYGKRQLREIDARIRFLAKRLDELTVVDRPPRDQGKVFFGAWVRL
                     ADETGEEARYRLVGSDEFDRAPEFISIDSPVARLLLGKSVGDEVDLPGAGGERTWEVV
                     AIGYEPA"
     gene            complement(6702..8891)
                     /locus_tag="AC16PP_09445"
     CDS             complement(6702..8891)
                     /locus_tag="AC16PP_09445"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6481893.1"
                     /translation="MKSQIDQFPKYFPGNSRSLLSNFDQHDIDSDSVRTGLHFLPSDR
                     LEINLTANYSTVDQAGPTRKTAGLYYTPDAALLPNYENAIHDNLVEDPGFWKSDIWGV
                     TGRIDRDFNDTMTFTSLTSFREVDSELNWVIATEDVARLRLSTGTLPLAVYGSNDYVD
                     DSETFTQEFRRTSPGSGPFQWVGGLYYLNEETYRDETVPAGLLVPDGDGGALVLVPAV
                     DAGDKQDNTTDSYAIFGQVTWDITDKLAITAGGRQDWEEKKISRLRTPNGLAPARIFD
                     FSTSEDWSAFTAKGGIEFQATDDLFFYATVCKGFKSGGYQGSAATELAAITPFEPEGS
                     PFGTPGQWVIGMGYDDSLIEEQRHPERSDLDAISSEHPIALIHVSGHLTTVNSMALDV
                     SGIAAETPDPPGGMIRRQPGSNEPNGVLEETATAPLRHIYSGGYGGGGGAPGADLRKA
                     LAMYAENGVTAIQDGGASWEIHEMVREASIAGDANLDVVLYPVGMDPTFSVPEGVTLG
                     EYQDRVKVGGIKLLLDGSPQGKTAYLSKPYEVAPHGQPDDYRGYPMIPQAMADERVAH
                     FIGRGIPMLVHCNGDAAAEMLLDALDKANETMDLGDHRTVMIHAQTVREDQLDRMAAL
                     GVVPPDMMRLLWATVNRITRSGQTLGEAQRISALEAIRAVTVHAAYQNFEEDLKGSLT
                     PGKQADLVILSANPFTVDAQQILDIEVVETISRGVTVYQEPSLSASL"
     gene            9182..10489
                     /gene="aptA"
                     /locus_tag="AC16PP_09450"
     CDS             9182..10489
                     /gene="aptA"
                     /locus_tag="AC16PP_09450"
                     /EC_number="2.6.1.-"
                     /EC_number="2.6.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     S-adenosyl-L-methionine:8-amino-7-oxononanoate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0016223 - beta-alanine:pyruvate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omega-aminotransferase AptA"
                     /protein_id="HIR6481894.1"
                     /translation="MSRADLENHWLPFTDNKTFKDDPRLVVRGEGAYLWNQRGEKLLD
                     GSAGLFTCALGHCRPEITEAVSAQLAELDYIPSFLRSHPRAFEAANLLTRILPDPINH
                     VFFCNSGSEAVDTAMKIVLQYQLARGQAQRSVFVTRDRAYHGVNLGGTALSGIARNRQ
                     AFGSLVPGIVHMRDTWDADQCFSRGQPARGGVDLADDLHRAVKTHGPNRIAACFVEPI
                     AGSTGVLVPPAGYLERLREICDEHDILLVFDEVITGFGRTGAAFGADSFGVTPDIMTM
                     AKALTNGAQPMGAVAVRDEIYQTVIDASPDDAVEFAHGYTASAHPAACAAAIATLGIY
                     EREKIFERAREMSEYFLDSVFALQNLPAVADIRGFGMLAGFDVAAEGTPGLRGMRLQR
                     ALFDAGLHVKTTGDAAILAPPLVIEKSQIDEMCETLRDVLKSI"
BASE COUNT         1734 a         3385 c         3593 g         1927 t
ORIGIN      
        1 ggccgagccg tggcgaccct ggcgggcgta cgcggtcatg catctctggt ctgctcccgt
       61 gccgtcggtc gatgcggtcc ggttacaatc ggtgtcgggg acgtgacccg caacgacggg
      121 gaagggcaca tgtactacac ctatatggac ggcccggtgg gccgactgct gctcgccggt
      181 gacggcaagc gcctgaagct gatcggattc tccacgggcg cccgggcgcg cggtgcgcag
      241 caggactggg aacgctacga cgagccgttt cgcccggcga aacgccagct cgacgagtac
      301 ttcgacggcc ggcgccgcta cttcgacatc gagcttgcgc ccgacgtgac cccgtttcag
      361 ggcaaggtgc tggaggcgct gctcgagatc ccgtatgggg agacacgttc ttacggcgag
      421 attgcgcggg ccatcggtca gcctcgcgcg gtgcgggccg tcggcggggc caacggcagc
      481 aatccgatcc ctatcgtgat cccctgccac agggtcatcg gcagcaacgg ctcgctcacg
      541 ggctttggcg gtggcctgga caccaagcag tacctcctgg acctggagcg cagccactcc
      601 ggactcttcg agggtctcgt cggtgacagc cagcaggcgc cccaacgacc cgtgcgtgcg
      661 ggcaaagggc aggtggtgtt cacggtcacc cgctagacgg ctcgctcagt tcttgtcgat
      721 cctccagaac ttggagcctt ccagcgaaag atcgtacatc aggcccttgt tgccgtagac
      781 gaagccgatg atcgggtcct gcagggtgtg ggtgtcgaaa tccttgccga cgccgaactc
      841 gatgaaggtg atggccccgt ccacgccggc ctcccagccg tcactgttgc ggaagccgtc
      901 cagcgcttcg gatgacatga acagcaagac ctcggatttg gcctggccgc cgagctggaa
      961 accgaacgac gcggccgtca cccggtagta ctgcaccgcc tgattgttga tcagcagcgc
     1021 gccctcgccg tactcgccgc ccagcccgaa cccgagcttg tagacgcgcg gcatcaccag
     1081 cacgccggag gcttttgccg ccagttcccg cgcggcgggc tcgatctcgt agagcgattc
     1141 aagggcggcg gagatgcggg cgtcgatctc gaccttggag gcagaccatg cggccccggc
     1201 aggtgcaagg cagacggcgc aaagggtgag gaatcgaagg aacgaacgca tcgggcatca
     1261 tcctgctcgg ttgagaccgg ggcgttttat accaccgggc ggagccttgg tcgagggcag
     1321 aaattatgta accaattgtt tactatacct gtcgcgaagg gccggcaagc gaatgatcca
     1381 gataccggcc gaacgttcgc tgacggagcc caggcgaagt tgtaactaaa tggatacata
     1441 tgatcttttc agccgctgac tggcagtatt gagtaaccaa atggatacaa acggcctcag
     1501 gcgtgacccc gagggagctc gccggcgaat tctggattcg gctgccgagc acttctcgcg
     1561 ccatggcccg gacggtgccc gggtggacgc catcgcggcc gccgcccgtg tcaacaagcg
     1621 gatgctctat cactactttg gtgacaagca ggcgcttttc cgggcggtcc tggacgatcg
     1681 cgcgacgcgg cctggcggca cagggaacac gtccggctgg gataccacga cggcgcgcct
     1741 gctggtctgg gcagccggtc tggatccggt cggggcgacg ccgggaaacc tggagcagtg
     1801 gacggcgctg gccaggcacc tgggtgatga gcaggacagg ggccggttgc gcaacgatat
     1861 ccgggccggt accgcggcga tgctttatct cgccttggcc gccttgcccg cgctgttgcc
     1921 ggctcacgtg cgggccatct taggctccaa cgatgccgcg atgctgcagg gggaccttag
     1981 aaagcttctg aatccgcccg ccgagctcca cggcggcagc gggcgtccca gggtacgcat
     2041 gcagccccag gttcgtccgc gctgaggtca gcggcgtgcg agacgctcga actccgcgaa
     2101 gtagcgcgga aacgtcttgc tgacgcagtc cgggtcgttg atcgtgaccg ccacgccgcc
     2161 caacgcggcc agcgagaaac acatggccat gcggtggtcg tcgtaggtgt cgatagaggc
     2221 tgcgttcatg tattttggag gattgactgt aagtgagtcc tcacattcat ctacctcggc
     2281 acccagcttc ttaagctccg tcgccatggc ggcaaggcgg tccgtctcct tgacccgcca
     2341 gttgtatatg ttccggattc gtgtggaacc ctcggcgaag agcgcgagca cggccagcgt
     2401 catggcggca tcggggatgt ggttcaggtc cagatcgacg cccgtgagtg ctcccggctc
     2461 gacccgcacc cattcggcgt ctacggtcac ccgcgccccc atggccttta ggacctcgac
     2521 gaaggccatg tcgccctgga cggaacctgc tccgatgccg tgcacgcgga cgccccggcc
     2581 acggatggcg cccgcggcga ggaaatagga agcggcggat gcgtcgcctt cgatgaggta
     2641 gcgcccgggc gattggtagc cgccgggctc gacgcgaaac aggcgatgat cctcgtggcc
     2701 gacggtcgcg ccgaagcggc gcatcatgtc tacggtgatg tcgagatagg gcttggacac
     2761 ctgctcgccc tccacgacga cctccaccgg tcccgcggcc aggggggcgg cgagcagcag
     2821 ggaggtcagg aactggctgg acacgttgcc ctgcatggtc acgcgcccgc cgagaaggcc
     2881 ggtgccggtc acctcgatgg gcgggaagcc ctccgcgccg gtgtagcgga tgctcgcgcc
     2941 gagctggcgc agcccttcca ccaggtgccc gacgggccgt tcgcgcattc tcgcggtgcc
     3001 gtccagctcg aatacgcctc ggcccagggt cagtgctgcg gtgagtggcc ggagagcggt
     3061 gccggccagg ccgagatcca gagactcgtg gcgctcgtcg ttgaccaggg gcccggccac
     3121 gccggtcacg agatggtcgc cgccgttctc ggttatggat attcccagct gccccagggc
     3181 gcgaagcatg taggccgtgt cgtcgctgtc gagcaggttc accaggcggg tctggccttg
     3241 ggcttcagcg gccaggagca gggcgcggtt cgaaatgctc ttggagcccg gcagcgtgat
     3301 gtcgccttcc agagcctgga tgggttgaat ggtcagagat cccatggcgt gggattaaac
     3361 gacaaaccgg gtgcgggcgc gaacctgcgc gttaccgcag cagactgcgc tcagggtgca
     3421 gctccggagg tgcgggcacc agcgagtcga tgaagctggc gaggcttccg gccaccacgt
     3481 ccaccggctt gtgaccgggc ttctcgatca gcacctcacc ggtggtgttc tccacggaga
     3541 gaaagagctc cgagtcctga tcggtgcagg cgaagaagac gctgagaggc gccctggctc
     3601 gccgtttggc cagcgcgtgc ccgatgaggt tctcgatcag ccgggaagca tcctctgcgt
     3661 tccacagaag aatgaggctt acgtgaccct ggggcgcctc tgcctcgagc cctcccgacc
     3721 agaagctgcc gtagtaagcc ttgatgtccg ggtgtatttc agtctccagg gcgttctcca
     3781 ggccggcgaa gtcgtgctcc tcgggaactt tcagcccgcg ggcaagcggg ctccagggta
     3841 cccggacgat gccgtcgtcg tcctggtagg gctcgctcgt ctcgcagggc gagcgccatt
     3901 cgggatcgaa tatctccgta agctcggggt aggacttctc gtagtgggag acgaattggg
     3961 aaagcgcggt ctggacggac aacgaagatc tcgcttggaa tcgaggcggt attttacctg
     4021 ctacgggcgc acggcaggca tgccgggggg cgggtgcccg tgaggccgat ggcggcctga
     4081 gcccgccagc gccttcccga gcaggcgacg ctcctcgggt gagagagatc gcgcaagccc
     4141 gacgaaggcg gcgtgactgg tttcctggct gcggctcaga tgggtacgga acgccccaag
     4201 cgcctcctcg agcttttcgg ggtggaacgg ctcggccacc agcgcggctt cgatatcccg
     4261 ctgagcctca cggatcccgc cgacgcttgg ccggatcgac ctgacctggt cgcgcacgag
     4321 cgggcgcagc gcctcgcggc gctcctccgg aagctgttgc agaagcatcc gggtcaccct
     4381 cagattggga tcgagcagac gaggtccgcc cccatcaccg gttgcgcgtc ccgccaggaa
     4441 tccggccatg gcgaggtttg cgcccagcga caggaccagc agaagaacga gccatttgct
     4501 acggggcatc ttcgaactcc agataggtct cggtaaacgc aagccctgcg acgtcatcga
     4561 tcagggcttc ttcgtttgcc gccggcagca gcgtgcccgt ggcgaagccc agcagcagcg
     4621 cgcaagcgcc ggtcagcgcc ggtggccaga acgcggaggc gaaccagtcg gccgcgcgct
     4681 gccagatgtc cgggcgcggc agctggtcga gaatcttctg tttgagctgc atcggcgcgc
     4741 gatgagttcg gccctcgtcg agccagccat ccagagcctg cccctgaacc atgagcgccc
     4801 gggcccgctc cgaacgttcc accagcatcg acgcggcgtc gcgttccgga tccggccagg
     4861 agtcgagatc ggatccccag cggtccaggc cctgttcgaa gtcgtcgatg ttcaacgcgt
     4921 ggctcccctt gttaacccgc tcattcgctg aggtcggcca accgtaatct gaggttgcgc
     4981 cgggctcgcg ccagcagcga ctccagggcg tggacgctga cattcattat ccgggccgcg
     5041 tcctggttcg aaaaaccctg aatccggctc agcacgatgg ccgagcgctg cgcctcgggc
     5101 agcgagccga gcgcggcctg cagatgctcg agccgctcgg tggcctggtg cacgccacgc
     5161 tcgtcgaccc cgggttcggg cgcttcgtct aggctcgtcg tccggcggcg gcgtcgcaac
     5221 tcgtcgacgc agacgttgtg ggcgatgcgg tgcatccagg tggttaccgc cacccggccg
     5281 gcctcgaagg tgcgggcatt gagccatacg cgcaggaacg tctcctgggc gagttcgtcg
     5341 gcgtcggcgg tcgagccggc gagacggacg aggtagcggt gaatggaatc gagatgacga
     5401 gtcagcagat ggctgaacgc ctcgtgatcc tgcttggcta tcagcaccat cagcgcttcg
     5461 tccgtcggct ctcccaccct cgagcgtgtc gggtcagccg ccgggcccgc ggtcgccgcc
     5521 gtgatgctcg cgcatcgcgg ctttcatctc gtcgcgggtg acgttcccat caccgtcggc
     5581 gtccactttc ttcaaacgct cgagacgacg gtccagcaac tcatcgttcg tgagcacgcc
     5641 gtcgtcgttg ctgtccagac gcgcgaacgt ctcttccctg cgcagacgct gatggttctc
     5701 gtgcatcttc tcgtggctga actcgccccg ggacagcttg ccgtcgccat cggcgtcgag
     5761 ggcgtcgaac aacgcggcgt ccagggcggc ctggtcgggt ggcggcccat cgccgtgatg
     5821 gcccggtcga tgatgcgctc cccgttcggc gcgatgggta ccggagtgag cgtgatggcg
     5881 ccctttcggc atcggccgct ccgcgttggc gaattcctcg ctggtgatct ttccgtcgcc
     5941 gttcgcgtcg atttctccga acgcctcggc cgcgcgggcc tctgcttccg cgatggatat
     6001 cgggaagtcc cggtcgtggt gatggctcca gctcgtcccg gtggcgagac cggtcagggc
     6061 gagcagagca agggtgatgg tcgtcttcat ggtcattcct gttggtttcc tggcggttat
     6121 acgcacggag tcgaaaaacc cgtcgctcgt caggctggct cgtagccgat ggccacgacc
     6181 tcccacgttc tctcgccgcc ggctccgggc aggtcgacct cgtcccccac cgacttgccc
     6241 agcagcaacc gggcgacggg ggagtcgatg ctgatgaact caggcgcccg gtcgaactcg
     6301 tccgagccca ccagccggta gcgggcctct tcgccggtct cgtccgcaag cctcacccac
     6361 gcgccgaaaa agaccttgcc ctggtccctc ggggggcggt ccaccaccgt cagctcgtcc
     6421 agccgcttgg cgagaaaccg gatgcgggcg tcgatctctc tgagctgccg cttgccgtag
     6481 atgtactcgg cattctcgga acggtcgccc agcgcggctg cttcagcgac cttttgggtc
     6541 acctcggggc gtttgacctt ccacagctcc ttgagttcgg cagccagacg ctcctggccg
     6601 gccggcgtga tgtaggggga ggatctgggc cggggcgggc ggtagcggct catcggcgcc
     6661 cgtgcgttaa ggtttttttg ccagcagaca ttgggtccgg cctaaaggct cgcggagagc
     6721 gagggttcct ggtagacggt aacaccccgg gagatcgttt ccaccacctc gatgtccaga
     6781 atctgctggg cgtccacggt gaacgggttg gccgagagga tcaccagatc cgcctgcttg
     6841 cccggtgtca gggacccttt gaggtcttcc tcgaaattct ggtaggcggc gtggacggtc
     6901 accgcgcgga tcgcctccag ggcgctgatg cgctgagcct cgcccagggt ctggccgctg
     6961 cgggtgatgc gattcaccgt ggcccacagc agacgcatca tgtcgggcgg caccacgccg
     7021 agtgccgcca tgcggtcgag ctgatcttcc cgaaccgtct gggcgtggat catcaccgtt
     7081 cggtgatcgc ccagatccat ggtctcattc gccttgtcca gggcgtcgag cagcatctcg
     7141 gcggcagcgt cgccgttgca gtgcaccagc atggggatgc cgcggccgat gaagtgggcc
     7201 acccgctcgt ctgccatggc ctgcggaatc atgggatagc cgcggtaatc gtccggctgg
     7261 ccgtggggcg ccacctcgta gggtttgctc aggtaggccg tcttgccctg gggcgaacca
     7321 tccagaagca gcttgatgcc accgaccttc acccggtcct ggtactcgcc cagcgtgacg
     7381 ccttccggaa cggaaaacgt cgggtccata cccacggggt acagcaccac gtcgagattg
     7441 gcgtccccgg cgatggatgc ttcccggacc atctcgtgga tctcccagga agcgcccccg
     7501 tcctggatcg cggtgacccc gttctcggcg tacatcgcaa gggccttgcg caggtccgca
     7561 ccgggcgccc ccccgccgcc gccatagccg ccagaataga tgtggcgcaa cggcgcggta
     7621 gccgtctcct ccagaacgcc gttgggctcg ttgctgcccg gctggcggcg gatcatgccg
     7681 cccgggggat cgggcgtctc ggccgcgatg ccgcttacgt ccagcgccat ggagttcacg
     7741 gtggtcagat gaccggaaac gtggatgagc gcgatgggat gctcgcttga gatcgcgtcc
     7801 aggtcggaac gctccggatg gcgctgctcc tcgatgagcg aatcgtcgta gcccatgccg
     7861 atcacccact ggcccggcgt gccgaaaggc gagccctcgg gctcgaaggg cgtaatggcg
     7921 gcgagctccg tggccgcgct accctggtag ccgcctgact tgaacccttt gcagacggtt
     7981 gcgtagaaga acaggtcgtc ggtcgcctga aactcgatgc cgcccttagc cgtgaaggcg
     8041 ctccagtcct cgctggtgct gaaatcgaag atccgcgccg gcgccaggcc gttcggcgtg
     8101 cgcaaccggc tgatcttctt ttcttcccag tcttgacggc caccggcggt gatcgccagc
     8161 ttgtccgtga tgtcccaggt gacttgaccg aatatggcgt agctgtcggt ggtgttgtcc
     8221 tgcttgtcgc ccgcatccac ggccggcacg agaacgaggg cgcccccatc gccgtcgggc
     8281 accagcaggc cggcgggtac ggtctcgtcg cggtaggttt cttcgttcag gtagtagagc
     8341 ccgcccaccc attggaaagg cccgctgccg ggcgaggtgc gcctgaactc ctgcgtgaag
     8401 gtttccgagt cgtcaacgta gtcgttgctg ccgtagacgg caagcggcag cgtcccggtg
     8461 gagagtcgca gccgggcgac gtcctcggtg gcgatgaccc aattcagctc cgaatccacc
     8521 tcgcggaagg aggtcagcga ggtgaacgtc atggtgtcgt tgaaatcccg gtcgatgcgc
     8581 cccgtgacgc cccagatgtc gctcttccag aagccggggt cctccaccag gttgtcgtgg
     8641 atcgcgttct cgtagttcgg cagcagcgca gcgtcgggag tgtagtaaag cccggcggtc
     8701 ttgcgggtcg ggccggcctg gtccaccgtg ctgtagttgg cggtcaggtt gatctccagg
     8761 cggtcgctag ggagaaagtg cagaccggtg cggacgctgt cggagtcaat gtcgtgctga
     8821 tcgaaattgc tcagcagcga tcggctgttg cctggaaagt acttcgggaa ctggtcgatc
     8881 tgcgacttca tgtagccgtc acgtttgcgg ctggagaagg ttgctgctat cgaacattga
     8941 acgttgtttt tagcgagccg gtcctccggc tcctgtcgat gcggcgcact ctgccgggct
     9001 tgaccccgtt cgtccagttg ccctgttgcg gtccttgaag cggcaggttc cgctgtacag
     9061 accagcgcaa gggtcattat atttcgcggt tcgatgggcc tggctggtga gtttccgggg
     9121 gaagcgataa gtgagcgaat tactgaatca gtccggggcc ggtgctcggc caggtccggc
     9181 gttgtcgaga gccgatctcg agaaccactg gctgccgttc acggacaaca agacgttcaa
     9241 ggacgacccg cgtctggtgg tgcggggcga aggcgcctac ctgtggaacc agcggggcga
     9301 gaagctgctg gacggctccg cggggctgtt cacctgcgcg ctcggtcact gccgacctga
     9361 gatcacggag gcggtgagcg ctcagctggc cgagctcgat tacatcccgt cgttcctgcg
     9421 cagccatcct cgggcgttcg aagcggccaa cctgctcacc cggatcctgc ctgaccccat
     9481 caaccatgtt ttcttctgca actcgggttc ggaagccgtg gatacggcca tgaagatcgt
     9541 gctgcagtac cagctggcta gaggccaggc ccagcgtagc gtcttcgtca ccagagatcg
     9601 ggcctaccac ggggtgaacc tggggggcac ggcgttgagc ggcatcgccc gcaaccgtca
     9661 ggcgttcgga tccctcgttc cgggcatcgt gcacatgcgt gacacgtggg atgcggacca
     9721 gtgcttcagc aggggccagc cggcccgtgg cggtgtggat cttgccgacg atctgcatcg
     9781 agcggtgaag acccatggac cgaaccgcat cgccgcctgc ttcgtagagc ccatcgcggg
     9841 ctccaccggc gttctcgttc cgcctgcggg ttatctcgag cgcctgcggg agatctgcga
     9901 cgagcacgac atcctgctgg tgttcgacga ggtgatcacc gggttcggcc gaacgggggc
     9961 ggcctttggt gcggattcct tcggcgtcac gccagacatc atgaccatgg ccaaggccct
    10021 gaccaacggt gcccagccca tgggcgccgt cgcggtgaga gacgagattt accagacggt
    10081 gatcgatgcc agtccggacg acgcggtgga gtttgcccac ggctacacgg cctcggccca
    10141 ccctgcggcc tgcgccgccg ccatagcgac cctggggatc tacgagcggg agaagatctt
    10201 cgagcgtgcc cgggagatgt ccgagtactt tctggattcg gtgttcgccc tgcagaacct
    10261 gccggcggtg gcggacattc ggggattcgg catgctggcc ggcttcgacg tggccgcgga
    10321 aggcacgccg ggactgcgtg gcatgcgtct gcagcgggcg ctgttcgacg cgggcctgca
    10381 tgtcaagacc actggggacg cggcgatact ggcgccgccg ctcgtcatcg agaagtccca
    10441 gatcgacgag atgtgcgaga cattgcgaga cgtgctcaag tcgatctgat gaggccaagc
    10501 gggatcgcct ggacaactca tgagccgggg gaggacggat gagcgaagaa gcggaaggcg
    10561 tacgcgtata ccgcggcctg aagggcgtgc actttgatcg ctcgccaacc acttacatcg
    10621 acgggcgagc gggcgagct
//
LOCUS       DBNHKV010000301         3172 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_184222, whole genome shotgun sequence.
ACCESSION   DBNHKV010000301 DBNHKV010000000
VERSION     DBNHKV010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_184222"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1182
                     /locus_tag="AC16PP_09455"
     CDS             256..1182
                     /locus_tag="AC16PP_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6481895.1"
                     /translation="MSHLELRHLRTLVALRDTGSLVEAAERVFLTQSALSHQLKDLES
                     RLGVTLFIRKTRPVRFTTAGRRLLKLADQVLPLVHGAEHDLERLAGGESGRIYMAIEC
                     HSCFEWLLPAINAYREEWPDVELDIASGFHFAPLPALARGDLDLVITADPIEELGLVY
                     LPLFSYEAQLAVARDHELADKPWVEPADLSREVLICYPVDRSRLDIFTSFLDPANVEP
                     ARVRNAELTTMMIQLVASGRGVACLPNWALHEYRQRDYVQVKSLGEDGVWPTLYAAVR
                     ADQADAAFIRGFIDVAKQTCFQNLVGIVTADV"
     gene            complement(1215..1790)
                     /locus_tag="AC16PP_09460"
     CDS             complement(1215..1790)
                     /locus_tag="AC16PP_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6481896.1"
                     /translation="MDPLAQLTRDRAEARRLEDPCANLCTVASVDSAGNPQARTLVLR
                     DVNERLAIFANETSPKWREISLNERISVVVWLPVLNLQYRLRCGTERVPTHLVHDRWH
                     QRPDPPKRLDWFYTNHLPQSSPLPDRDTLLERLAQIDLPEPLAPPQTAAGLYLSPTTV
                     ERLDLNQSNGIHDRRHFELSEDGWVQSVLVP"
     gene            complement(1840..2031)
                     /locus_tag="AC16PP_09465"
     CDS             complement(1840..2031)
                     /locus_tag="AC16PP_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481897.1"
                     /translation="MVFLCFIVLLGIAYLVWRVTDQMPDLIFRLSEVQRDVAEIRRRL
                     TEESDAPAGAAQGGGDAEA"
     gene            complement(2269..2763)
                     /locus_tag="AC16PP_09470"
     CDS             complement(2269..2763)
                     /locus_tag="AC16PP_09470"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SixA phosphatase family protein"
                     /protein_id="HIR6481898.1"
                     /translation="MKIWIIRHAKSSWAQPGLSDFDRPLNDRGRADGPRMAAWLASQD
                     APAEWIWTSDAARARATAAFVERGFSAARPLVVEEHALYHASARGALDVLRGTPEAVA
                     SVALVAHNPGLTDLVNLLAGNLVTENLPTFGVARFDVAAPWHSVGEQRADLEILTAPK
                     EIPS"
     gene            complement(2775..>3172)
                     /locus_tag="AC16PP_09475"
     CDS             complement(2775..>3172)
                     /locus_tag="AC16PP_09475"
                     /inference="COORDINATES: protein motif:HMM:NF025178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR6481899.1"
                     /translation="PLHQRAAVSEAMSEAKQINLGPLSDLEEGDKRSFDAEGTQVLVC
                     RVRGELYAIEDLCSHANKPLCRGRLSGYMITCPVHSARFDIRDGAHQGPPAFTGIRTF
                     PIEEGADGAIVTVTPKPLPAGFGGPLMTR"
BASE COUNT          520 a         1004 c         1053 g          595 t
ORIGIN      
        1 gggctgcgcg agcgtgttcg cgaacgcctc gacataagcg ttcgtgcaat gtgcggcaaa
       61 agcttcaacg acgattggat tcgggttgaa catcaagcag tccggacaat tcgaggcagt
      121 ttaaggcacg gtccccccag cctgcgtgcc tcagtcctca ttctatgcca tatcgggtac
      181 cgggagattg agattgaggc ctcgtcgttt tatgaatatt ctgcacttaa ggaaagaatt
      241 tcgtgaggga aattcatgtc ccatctggag ctcaggcacc tgaggaccct cgtagcgctg
      301 cgggacacgg gcagcctcgt cgaggcggcg gaacgggttt tcctgaccca gtccgcgctt
      361 tcccaccagc tcaaggacct ggagtcccga ctcggcgtca cgctgttcat ccgcaaaacg
      421 cgacccgttc gcttcaccac cgccggccgc cgactgctca agctcgccga ccaggtgctg
      481 cccctcgtcc acggcgccga gcacgacctg gagaggctgg ccggcggcga gtcgggaagg
      541 atatacatgg ccatcgagtg tcacagctgc ttcgagtggc tgctgcccgc catcaatgcc
      601 tatcgcgagg agtggccgga tgtcgagctg gacattgcca gcggctttca cttcgctccg
      661 ctccccgcgc tcgctcgcgg cgatctcgat ctggtgatca ccgcggaccc cattgaggag
      721 ctcgggctcg tctacctgcc gctgttcagc tacgaagcgc agctcgcggt tgcccgtgat
      781 cacgagctgg cggacaaacc ctgggtggag cccgccgatc tgtcgcgcga ggtgctcatc
      841 tgttatccgg tggaccgcag ccgcctcgac atcttcacca gcttcctcga tccggccaac
      901 gtcgagccgg ctcgtgtgcg gaacgcggag ctgacgacca tgatgatcca gctggtggcc
      961 agcgggcgcg gcgtggcatg cctgcccaac tgggctctgc atgagtatcg ccagcgcgac
     1021 tacgttcagg tgaagagcct gggagaggac ggcgtctggc ccacgctcta cgcggcggtc
     1081 cgggcggatc aggcggacgc cgccttcatt cgaggcttca tcgacgttgc gaagcagacg
     1141 tgcttccaga acctggtcgg tatcgtcacc gcggacgtct gacaggatcg acgacctggt
     1201 cgcggtggcg gtgatcaggg aaccagcacc gactgcaccc agccgtcttc cgacagctcg
     1261 aagtgccggc ggtcgtgaat gccgttgctc tgattgagat ccaggcgttc gaccgtcgtc
     1321 ggcgacaggt agagtccggc cgccgtctgc ggaggtgcca gcggctcggg aagatcgatc
     1381 tgggccaggc gctccagcag cgtgtcccgg tcgggcagtg gggaactctg cggaaggtgg
     1441 ttggtgtaga accagtccag ccgcttgggc ggatccgggc gctggtgcca gcggtcatgc
     1501 acgaggtgcg tcggcacgcg ctcggtgccg caccgcaggc ggtattgcag gttcaggacc
     1561 ggcagccaga ccaccaccga aatccgctcg tttaagctga tttcccgcca tttggggctc
     1621 gtctcgtttg cgaaaatcgc caggcgttcg ttgacgtcgc gcagcaccag cgtccgtgcc
     1681 tggggatttc ctgcggagtc gacggaagcc accgtgcaga ggttggcaca ggggtcttcc
     1741 aggcgcctcg cctccgcgcg gtcgcgggtg agttgggcaa gcgggtccac taaaagcccg
     1801 gtggtgcttc gttcgatggc gggcgccggc ttgccgacgt caggcctcgg cgtcgcctcc
     1861 gccctgagcg gccccggccg gtgcgtcgct ctcctcggtc aggcggcggc ggatctccgc
     1921 gacgtcgcgc tgcacttcgc tcaggcggaa aatcaggtcg ggcatctgat cggtgacgcg
     1981 ccacacgagg taggcgatgc ccagcagcac gataaagcaa aggaagacca tgcacatctc
     2041 cggttggttg ggggactcgc gattaggtat accgactcca tcgccgggag tccagcggat
     2101 gaacgtgaac gcggctgccc tttgggtttc cagcgcaacc gtttcaccgc tcgtatgcag
     2161 cgtgggctga ttgcctccgc gtcgcgggtc atgaaatctg gtgcgctatc gagtcgttcc
     2221 cgtcgagctc tgcaacggtt gcttcctgtt caggatcgac aaaccaggct aggacggaat
     2281 ttcctttggc gccgtcagga tttccagatc tgcgcgctgc tcccctacgc tatgccatgg
     2341 ggccgcgacg tcgaagcgcg ccacgccgaa ggtcggcaga ttctccgtca ccaggttgcc
     2401 ggccagcagg ttgaccaggt cggtgagacc agggttgtgg gccaccagcg ccacgctggc
     2461 aaccgcttcc ggagtgcctc tcagcacgtc caaggcgccg cgcgcgctgg cgtgatacag
     2521 ggcgtgctcc tctacgacca gcgggcgtgc ggccgagaag ccccgctcga cgaaagctgc
     2581 ggtggctcgg gcccttgcgg cgtcgctggt ccagatccac tctgcgggcg cgtcctggct
     2641 tgcgagccag gcggccatgc ggggcccgtc ggcccggccg cggtcattga gtggccgatc
     2701 gaagtcggac agacctggct gcgcccatga tgacttggcg tgacggatga tccagatctt
     2761 cacggggccg gcgttcagcg ggtcatcaag gggccgccga agccggcagg aagcggtttc
     2821 ggcgtcaccg tcacgatcgc gccgtccgct ccctcctcga tgggaaacgt gcgaatgccg
     2881 gtgaacgccg gtggcccctg atgggcgcca tctctgatgt cgaaacgggc gctgtggacg
     2941 gggcaggtga tcatgtagcc cgaaagcctg ccccggcaga gcggcttgtt ggcgtggctg
     3001 cagagatcct cgatggcgta gagctcgcct cggacgcggc agacgaggac ctgcgtaccc
     3061 tccgcgtcga aggagcgctt gtcgccttct tccaggtcgc tcagaggacc caggttgatt
     3121 tgctttgctt cgctcatcgc ctcgctcaca gctgcccgtt gatgtaacgg tc
//
LOCUS       DBNHKV010000302         4999 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_539141, whole genome shotgun sequence.
ACCESSION   DBNHKV010000302 DBNHKV010000000
VERSION     DBNHKV010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_539141"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1424
                     /locus_tag="AC16PP_09480"
     CDS             <1..1424
                     /locus_tag="AC16PP_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIR6481900.1"
                     /translation="APYRTAGKRLLDFQEIYTDHDEARLTNQGARCMDCGVPFCQSND
                     GCPIHNLIPEWNDLVYRHQWREALDRLHKTNNFPEFTGRVCPAPCEGACVLGITDPAV
                     TIKNIEMAIIDRGFEEGWVKPNPPSQRTGKRIAVVGSGPAGLAAADQLNKAGHTVIVY
                     ERADRIGGLLMYGIPNMKLDKAEVVERRLNLLREEGVEFVTNAHIGDGSQGTISGSDL
                     VNDFDAVLLATGATIPRDLPIPGRDLRGVHFAMEFLTANTKSLLDSGHADGAYISAKD
                     KDVIVIGGGDTGTDCIGTSLRHGCRSLVNFELFPKPPEVRGPDNPWPTWPRIFRTDYG
                     HQEAAERFGSDPRVYAISSTEFFGDEAGNLKGVRTVDVTLKDGRFENVPGSEREWPAG
                     LVFLSMGFLGPEHLVSDSLGIAYDDRSNYSAEYGRYATSVDKVFAAGDCRQGQSLVVR
                     AINEGREAAREIDRFLMGASQLP"
     gene            complement(1432..2517)
                     /gene="aroB"
                     /locus_tag="AC16PP_09485"
     CDS             complement(1432..2517)
                     /gene="aroB"
                     /locus_tag="AC16PP_09485"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020302367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR6481901.1"
                     /translation="MTLEVDLGDRSYPIHIGDGLLGRGELLRDQVHGGQVAVITNDVV
                     GPLYLPRVAQALAGIDADVYTLPDGEQHKNLESYGAIMDFLMERRHNRSTTLVALGGG
                     VVGDITGFVAATFQRGVAFVQVPTTLLAQVDSSVGGKTAINHPRGKNMIGAFYQPRCV
                     IADTTVLNSLGDRELRAGLAEVLKYGIIADAGFFDWLESNANALLSREPETLAVAIRR
                     SCEIKAAVVAEDERESGIRAILNYGHTFGHAIETLTGYGELLHGEAVAAGMVMAADLS
                     ARQGWLTGTEAKRIKAALEAFGLPVVAPSIEPDDMLSAMSIDKKVVNGRLRLVLARAI
                     GEAAVTEDVDEPALRATLAAGEALCDG"
     gene            complement(2523..3047)
                     /gene="aroK"
                     /locus_tag="AC16PP_09490"
     CDS             complement(2523..3047)
                     /gene="aroK"
                     /locus_tag="AC16PP_09490"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010955625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HIR6481902.1"
                     /translation="MRTQNLFLVGLMAVGKSTVGRCLARDLDMAFYDTDEVLEERAGA
                     EVAWIFDVEGESGFRDREQQVIEELTAKSGVVLATGGGAVLRAANRQNLSARGIVVHL
                     DSPLERLVERTARDNKRPLLQGGDARETLARLQRERAPLYAEIADYTFVTDRQGPKVL
                     ARTIERKLREDGLI"
     gene            complement(3110..>4999)
                     /locus_tag="AC16PP_09495"
     CDS             complement(3110..>4999)
                     /locus_tag="AC16PP_09495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HIR6481903.1"
                     /translation="PGVESALEDKHHNLGIGNARRMSVVSTKDRTRAIVNLTQLVSYE
                     TEIQGNTLYLTVGSGGVSGVPAPQEYSAVGDVSDRGERDFDDSQINNVDFRRGEDGSG
                     RVIIELSNPKAPVNVVSDGGRIRVEVRNTELPKDLQRRLDVTDFATPVQVVDALQEGP
                     HTTFTVTATGNYDYLAYQADNTLSINVNPLTEEEIETREDIFRYTGEKLSLNFQDIEV
                     RSVLQLIADFTDLNLVASDTVSGRITLRLKNVPWDQALELILKTKGLDKRQVGNVLLV
                     APAAEIAAREKLELENQKQISELAPLRTEFIQIRYASATELFELFNNAASENRESMLS
                     ERGSVIVDERTNAIILTDTAEKLEDFRRVIAQLDVPVRQVLIEARIVIATTSFSEELG
                     IRWGGAALKNNGASKLKYGGSLDTLTELQDTVNGGAFAPSHPNDLIVDLGVTAANATS
                     FGVGITGEDYLLDLELSALATDGHGEVVARPKVITADKSTAVIESGTEIPYQEASSSG
                     ATSTSFKDAVLSLEVTPQITPDDRVIMELNVKQDSVGDIFNGVPSIDTNEIETQVLVD
                     NGQTVVLGGIFSDTKNVSTTKTPFLGDLPYVGRLFRKELENEEKTELLIFITPKILQD
                     TVSAR"
BASE COUNT          905 a         1645 c         1526 g          923 t
ORIGIN      
        1 cggcgccgta ccggaccgcc ggcaaacgtc tgctcgattt ccaggagatc tacaccgatc
       61 acgacgaggc gcgcctgacc aatcagggcg cgcggtgcat ggactgcggc gttccgttct
      121 gccagtccaa cgacggttgc cccattcaca atctcattcc cgaatggaac gacctcgtct
      181 accgacacca gtggcgggaa gcgctggacc ggctgcacaa gaccaacaat ttcccggagt
      241 tcaccggccg cgtctgcccg gcgccctgcg aaggggcctg cgtgctgggg atcaccgatc
      301 cggccgtgac cataaagaac atagagatgg cgatcatcga ccgtggtttc gaggaaggat
      361 gggtcaagcc caatcctccg agccagcgaa cgggcaagcg gatcgccgtg gtcggatccg
      421 gccccgctgg ccttgcggca gccgaccagc tgaacaaagc cggccacacg gtcatcgtct
      481 acgagcgggc ggaccgcatt ggcgggctgc tcatgtacgg catccccaac atgaagctgg
      541 acaaggccga ggtcgtcgag cggcggctga acctcctccg tgaggagggc gtcgagttcg
      601 tcaccaacgc ccacattggc gacggcagcc aaggcaccat cagcggcagc gacctggtga
      661 acgatttcga cgcggtgctg cttgccaccg gcgccacgat accgagagat ctgcccattc
      721 cgggccggga cctgcgcggc gttcacttcg ccatggagtt tctcactgcc aacaccaaaa
      781 gcctgctgga ctcgggccac gcggacggcg cgtacatctc ggcaaaagac aaggacgtca
      841 tcgtgatcgg gggcggcgat accggcaccg actgcatcgg cacatcgctg cggcacggat
      901 gccggagcct ggtgaatttc gagctgtttc caaagccgcc ggaagtccgg ggcccggaca
      961 acccctggcc cacctggccg agaattttcc ggacagacta cgggcaccag gaggccgccg
     1021 agcgtttcgg cagcgacccc cgcgtctacg cgatctcttc taccgagttc ttcggcgacg
     1081 aagccggaaa tctgaaaggg gtgcggaccg tcgacgtgac gctcaaggac ggcaggtttg
     1141 agaacgttcc gggatccgag cgggagtggc cggccggcct ggtgtttctc tccatgggtt
     1201 ttctgggccc cgagcacctg gtctccgatt ccctcggaat cgcctacgac gaccgctcca
     1261 actactcagc cgagtacggc aggtacgcca ccagcgtcga caaggtgttc gccgccggcg
     1321 actgccgaca gggtcagtcc ctggtggtgc gagccatcaa cgagggacgc gaggctgcgc
     1381 gcgagatcga ccgcttcctc atgggcgcct cacagctgcc ctgacgggcg gtcagccgtc
     1441 acacagagct tcgcccgccg ccagcgtggc cctcagcgca ggctcgtcga cgtcttcggt
     1501 gacggccgct tccccgatgg cgcgcgcgag caccagtcgc aagcggccat tcacgacctt
     1561 cttgtcgatg gacatcgcgc tcagcatgtc gtccggctcg atggaaggtg ccaccacggg
     1621 caatccgaac gcctcgagcg cagctttgat gcgcttggcc tccgtacccg tcagccagcc
     1681 ctgccgagcc gaaagatcgg ccgccatcac catgcccgcc gccaccgcct caccatgcag
     1741 gagctcgccg taacccgtca gcgtctcgat ggcgtgaccg aaagtgtggc cgtagttcag
     1801 gatcgcccgg atgccggact cccgttcgtc ctcggccacc acggccgctt tgatctcgca
     1861 ggaccggcgt atcgccacgg caagcgtttc cggctccctg ctgagcagcg cgttggcgtt
     1921 gctctcgagc caatcgaaga acccggcgtc cgcaatgatg ccgtacttga gcacctccgc
     1981 gagcccggca cgaagttccc ggtctcccag cgagttcaga accgtggtgt ccgcgatgac
     2041 gcagcgtggc tggtagaacg ccccgatcat gttctttccg cgcggatggt tgatcgccgt
     2101 cttgccgccc accgacgagt ccacctgggc aagcagcgtg gtcggcacct gaacgaaggc
     2161 caccccgcgc tgaaacgtgg cggcaacgaa acccgtgatg tccccgacca ccccgccgcc
     2221 gagggccacc agcgtggttg agcggttgtg ccgccgctcc atgagaaaat ccatgatggc
     2281 gccgtagctt tccaggttct tgtgctgctc gccatcgggc agggtgtaga cgtcggcatc
     2341 gattcccgcg agggcttggg ccacgcgagg caggtacaag ggccccacca catcgttggt
     2401 gatcacggcg acctggccgc cgtgcacctg atcgcgcagc agctctccgc gtcccagcag
     2461 gccgtcacca atgtgaatgg ggtaactgcg atcccccagg tcgacctcga gcgtcacgtc
     2521 agtcaaatca aaccatcctc gcgcagcttg cgctcgatcg tgcgggccag caccttcgga
     2581 ccctgccggt cggttacgaa tgtgtaatcg gctatttccg cgtagagcgg agccctctcg
     2641 cgctgcaggc gagcgagggt ctcgcgggcg tcaccgccct gcagcagcgg cctcttgttg
     2701 tcgcgcgccg tgcgctccac gagccgctcg agggggctgt cgaggtgcac gacgatccct
     2761 cgagccgaca gattctgccg gttcgcagca cgcagcaccg cgccaccacc cgtcgccagc
     2821 acgacgccgc tttttgccgt cagctcctcg atgacctgct gctcccgatc gcgaaacccc
     2881 gactcgccct ccacgtcgaa gatccaggcg acctcggccc ccgcgcgctc ctcgaggacc
     2941 tcgtcagtgt cgtagaacgc catgtcgagg tcccgcgcaa gacaacgccc cacggtactt
     3001 ttgcccacag ccatcaaacc aacgagaaag agattctgcg tgcgcataaa aaaagcggcg
     3061 cctggagagg cgccgctagt ttagtttatt cgggctgggc ccgcgacgat caccgggcgg
     3121 acacggtgtc ctggagaatc ttcggcgtga tgaagatcag caactcggtc ttttcctcgt
     3181 tctccagctc ttttctgaac aatctgccca cgtagggaag gtcgcccagg aaaggtgtct
     3241 tggtagtcga tacattcttc gtatccgaga agatgccgcc gagaaccaca gtttgcccgt
     3301 tgtccaccag cacctgggtc tcgatctcgt tggtgtcgat ggacggaacg ccattgaaga
     3361 tgtcgcccac gctgtcctgc ttgacgttca gctccatgat gacacgatcg tcaggcgtga
     3421 tctgaggcgt tacctccagc gacagaaccg catccttgaa ggacgtggag gtggcgccgc
     3481 tgctcgaggc ctcctggtac ggaatctcag tacccgattc gatcacggcc gtcgacttgt
     3541 ctgcggtgat caccttcggc cgggcaacca cctcaccgtg gccatctgtt gccagggccg
     3601 agagctcgag atcgagcagg tagtcctcgc cggtgatgcc gacaccgaag ctggtggcgt
     3661 tagccgcggt gacacccaga tcgacgatca gatcgttcgg atgagacggt gcaaaggcgc
     3721 cgccgttgac cgtgtcctgc agctcggtga gcgtgtccag agagccgccg tacttcagct
     3781 tggacgcacc attgttcttc agcgctgcgc cgccccagcg aattcccagc tcttccgaga
     3841 aactcgtggt agcgatgacg attcgcgctt cgatcagcac ctgccggacg ggcacgtcga
     3901 gctgcgcgat cacgcgacgg aaatcttcga gcttctcagc ggtgtccgtc aggatgatgg
     3961 cgttggtccg ctcatcgacg atcacgctgc cccgctcgga gagcatggac tcgcgattct
     4021 cgctggccgc gttgttgaac agttcgaaga gctcggtggc cgatgcgtag cggatctgta
     4081 tgaactccgt gcgcagggga gcaagctccg agatctgctt ctggttctcg agctcgagct
     4141 tctcgcgggc cgcaatctca gcagccggag ccaccagcag cacgttaccc acctgccgct
     4201 tgtccagacc cttcgtcttg aggatcagct ctaaagcctg atcccagggc acgttcttca
     4261 accgcagcgt gatgcgaccg gataccgtat cgctggccac caggttgaga tccgtgaagt
     4321 ctgcaatcag ctgcagaacc gaacgcacct cgatgtcctg gaagttcagc gagagcttct
     4381 cgccggtgta cctgaagatg tcttcgcgcg tttcgatctc ttcttcagtg agcgggttga
     4441 cgttgatcga caacgtgttg tccgcctgat aagcgaggta gtcgtagttg cccgtagccg
     4501 tcaccgtgaa cgtggtgtgc ggaccttcct gaagggcgtc cacgacctgc accggagtgg
     4561 cgaaatcggt aacgtcgagg cgacgctgga gatccttcgg cagctcggtg ttgcgaacct
     4621 ccacacgaat gcggccgcca tcgctgacga cgttgacggg cgctttcgga ttggaaagct
     4681 cgatgattac cctgccgctg ccgtcttcgc cgcgtcggaa atcgacgttg ttgatctggg
     4741 aatcgtcgaa gtccctttcg ccccgatcgc tgacgtcacc caccgccgag tattcctgcg
     4801 gggcaggcac gccggacact ccgccggacc cgacggtcag atacagggtg ttaccctgga
     4861 tctcggtttc gtagctcacg agctgcgtga ggttgacgat ggcacgcgtg cggtccttgg
     4921 tggataccac ggacatgcgg cgggcgttgc caatacccag gttgtgatgc ttgtcctcga
     4981 gcgcgctttc cacgccggg
//
LOCUS       DBNHKV010000303         3575 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_680849, whole genome shotgun sequence.
ACCESSION   DBNHKV010000303 DBNHKV010000000
VERSION     DBNHKV010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_680849"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="AC16PP_09500"
     CDS             complement(<1..481)
                     /locus_tag="AC16PP_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HIR6481904.1"
                     /translation="MLKLIPLALLSLVLGSCANEPARPPAPDPGAPVVIAKSPNDERS
                     YRYLVLDNELRVLLVSDPAADMAAASLVVLRGSFHEPAEFAGLAHFLEHMLFIGTEKY
                     PEVDSFKQFVAAQGGSSNAYTANDHTNYFMEVRTEAFREALDRFAQFFISPLIDPAYT
                     "
     gene            complement(519..845)
                     /locus_tag="AC16PP_09505"
     CDS             complement(519..845)
                     /locus_tag="AC16PP_09505"
                     /inference="COORDINATES: protein motif:HMM:NF019301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HIR6481905.1"
                     /translation="MTVSPPLPLGSTASWFLASWTEFLGGILLLFGLGTRLVAKAKDI
                     LREHGDYRYLNGSGSIVKLNGGIEFAAIYIAMLLALLVIGGGRYLSVDYDVGLLGRTR
                     ASAQRE"
     gene            978..1859
                     /locus_tag="AC16PP_09510"
     CDS             978..1859
                     /locus_tag="AC16PP_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481906.1"
                     /translation="MAGLTLDDLAQALLAGSEPWTDPGALAARLDPDIPEDWRNLAAV
                     VVAAGATGTIGVGGGQGAGKSTLAAAMVEASRLAGRKAQACSLDDFYLTRQERLALAR
                     TVHPLFVTRGVPGTHDVDLALSVLRGLEGVGVTRVPVFDKGRDDRAPGPEWLAVEGPV
                     ESIVFEGWCLGAPPEPAERLIEPMNSLEREEDANRTWRSHVNDVLGESYQRLWSAMDR
                     LLYLQVPDMAAVHDWRAQQELALPPSRRMSGGQLDRFLAHYERLTRWMLETLPERADV
                     VVELGRDHRISGVRRNS"
     gene            1940..2467
                     /locus_tag="AC16PP_09515"
     CDS             1940..2467
                     /locus_tag="AC16PP_09515"
                     /inference="COORDINATES: protein motif:HMM:NF017269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HIR6481907.1"
                     /translation="MYASHTAKTLLAVALVAPALAVAGTSYDRARVVSVEPLYETVSY
                     EVPREECRMEEVRHTRSGSHRSYTAPIVGAIVGGAIGNAVGHKKRNKQIGTVVGAVLG
                     GSIGADIARRGHHHGHGPEYGLERVCRTVSDWHEEQRLLGYRVTYRYAGETYITQLDE
                     APGSSLPVRVQVTPV"
     gene            2517..2840
                     /locus_tag="AC16PP_09520"
     CDS             2517..2840
                     /locus_tag="AC16PP_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR6481908.1"
                     /translation="MVKGRSLMNLRCRGRWLALAVMMIPAIAGAAPSDVFEKALEPNR
                     FAPPPGISMEQAADMVRRDTGGRVLSATPTQRGDRRGYEIRVLLDGKRVKKVFVDSQA
                     KPPGQ"
     gene            2871..3542
                     /locus_tag="AC16PP_09525"
     CDS             2871..3542
                     /locus_tag="AC16PP_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6481909.1"
                     /translation="MRILIVEDEAILRDQLVSRLKSAGYVVDAAQDGEEGLYYGQEYD
                     YDAAIVDLGLPKIDGIDLIERLRGEERRFPVLVLTARGNWQDKVEGLEAGADDYLTKP
                     FQLEELLARLNALIRRAGGYASPVINEGALSVDTAKKEVRVEDAVVDLTAYEYRVLEY
                     LLLNAARVVSKAELTDHLYEQDFDRDSNVIEVFVGRLRKKLDPVNPIRTVRGQGYRFN
                     TEGSA"
BASE COUNT          652 a         1095 c         1213 g          615 t
ORIGIN      
        1 cggtgtacgc aggatcgatg agcgggctga tgaagaactg cgcgaatcga tcgagggctt
       61 cccggaaggc ctcggtcctg acctccatga agtagtttgt atggtcgttg gccgtgtacg
      121 cgttggacga ccctccctgg gcggcgacga actgcttgaa cgagtccacc tccggatact
      181 tctcggtgcc gatgaacagc atgtgctcca gaaagtgggc gaggccggcg aactcggcgg
      241 gctcgtgaaa gctccctcgc agcacgacca gcgaggcagc ggccatgtcc gctgccgggt
      301 cggatacgag cagcacccgc agttcgttat cgagaacgag gtagcggtag ctcctttcgt
      361 cgttcggact cttggcgatg acgaccggag ccccgggatc gggggccggc gggcgcgccg
      421 gttcgttggc gcacgatccc agcaccagtg aaagcagcgc gagcggtatg agtttcagca
      481 cgtttcggtt tccagttgtt gagcggtccg ggcggcgatc attcccgttg tgcggacgcg
      541 cgagttctcc cgagcagccc gacatcgtag tcgacgctga gataacggcc acctccgatc
      601 accagcaacg ccaggagcat ggcgatgtag atggcggcaa attcgatgcc gccgttcagc
      661 ttgacgatgc tgccgctgcc gttgagatac cgatagtcgc cgtgttctcg caggatgtcc
      721 tttgctttgg cgaccagccg ggtgccaagt ccgaacagca gcaggatgcc tccaaggaac
      781 tcggtccagg aagccaggaa ccacgaagcc gtcgagccga gcggcaatgg tggcgagacg
      841 gtcatgccag tcagctacgg ttgctatgat tggctggatg aggcttctcc catgtttttc
      901 agaattagac tcgaaggcgg agcagggttc cccggagggc ggtccatgat gcacaaaccg
      961 tcaggtgatg gaaaaccatg gcgggtctga cactcgacga cctggcccag gcgctgctcg
     1021 caggttccga gccatggacg gatcccgggg cgctggcggc ccgcctcgat cccgacattc
     1081 ccgaagactg gcgcaatctg gcggccgtcg tcgtcgctgc gggagcgacg ggcaccatcg
     1141 gcgtcggcgg gggccaggga gcgggcaagt cgactctggc tgcggcgatg gtcgaagcca
     1201 gccgcctggc cggacgaaag gcgcaggcct gctcgctcga tgatttctat ctcaccaggc
     1261 aagagcgtct ggctctggca cgaaccgttc atccgctctt cgtgacccgg ggcgttcccg
     1321 gtacccatga cgtcgatctg gcgctgtccg ttctgcgcgg gctcgagggt gtcggcgtca
     1381 ccagggtgcc cgtattcgac aaaggccggg acgatcgtgc gcccggcccg gagtggcttg
     1441 cggtcgaggg cccggtagag agcatcgtct tcgagggctg gtgcctgggc gccccgcccg
     1501 agcctgccga acgtctcatc gagccgatga actcgctcga gcgcgaagag gatgcgaacc
     1561 ggacctggcg ctcccacgtg aacgatgttc tgggcgagag ctatcaacgg ctttggagcg
     1621 cgatggatcg cctgctctac ctccaggttc cggacatggc cgccgtgcac gactggcgcg
     1681 cacagcagga gctggcgctg cccccgtcca ggcgcatgtc gggcggtcag ctcgaccggt
     1741 tccttgccca ctacgagcgg ctgacgcgct ggatgctcga aaccctgccc gaacgggcag
     1801 acgtggtcgt cgagctcggt cgcgaccatc ggatctcggg cgttcgcagg aattcctgac
     1861 ccgtaggccg tattcatgat ccgttcagtc gtcggtcctt aatctggcgg ccgtcattgc
     1921 agtcgaatgg agaatgacga tgtatgcatc ccacacagcg aaaactctgc tggccgtcgc
     1981 cctggtggcg ccggcactgg cagtggcggg cacgtcctac gatcgggcca gggtggtttc
     2041 cgtcgagccc ctttacgaaa ccgtctccta cgaggtcccg cgcgaggagt gccggatgga
     2101 ggaagtgcgg cacacccgaa gcgggtcgca tcgttcgtac acggcgccca tcgtcggcgc
     2161 catcgttggc ggcgccatcg gcaacgccgt gggccacaag aagcgcaaca agcagatcgg
     2221 caccgtggtg ggagccgtgc tcggcggcag tatcggcgcg gacatcgcaa gacgcggtca
     2281 ccaccacggg cacggaccgg aatacggtct ggagcgggtt tgccggaccg tcagcgactg
     2341 gcacgaggag cagcggttgc tcggttaccg tgtcacctac cgctacgcgg gggagaccta
     2401 catcacgcaa ctggacgagg cgccgggttc gagccttccc gtgcgcgtgc aggtcacgcc
     2461 ggtgtgaccg gggcaacgtt agaagggatg gaaattgcgg gtacaatgag cacgctatgg
     2521 tcaaaggccg ctctctgatg aacctgcgct gccgtggcag atggcttgcg ctcgcggtga
     2581 tgatgattcc cgcgatcgcc ggtgctgccc cgtccgacgt gttcgagaaa gccctggagc
     2641 ccaaccggtt tgccccgccg ccgggcatct ccatggaaca ggccgcggac atggtgcgcc
     2701 gggacacggg aggccgggta ttgtcggcca cgcccaccca gcggggcgat cggcgcggct
     2761 acgaaattcg cgtgctcctg gacgggaaac gggtcaagaa ggtgttcgtc gactcccagg
     2821 cgaagccccc cggacaatga tccggagcct tgtgaggtag cgaatcggtc atgcgcatcc
     2881 tgatagtcga agacgaagcg atacttcgcg accagctggt ttcgcggctg aagagcgcgg
     2941 gatatgtggt ggacgcggca caagacggcg aggagggcct ttattacggc caggagtacg
     3001 actacgacgc cgccatcgtc gatctaggcc tgccgaagat cgacgggatc gacctcatcg
     3061 agcggctcag gggcgaggaa cggcgtttcc cggttctggt gctgacggcg cgcggcaact
     3121 ggcaggacaa ggtggagggg ctggaggccg gagccgacga ctacctgacc aagccatttc
     3181 agctcgaaga gctgctggcc cgcctgaacg ctctgatcag aagggcgggc ggctacgcct
     3241 cgccggtgat caacgagggc gcgctcagcg tggatacggc caagaaagag gtccgcgtgg
     3301 aggacgccgt cgtggatctc actgcctacg agtaccgggt cctcgagtac ctgctgctca
     3361 acgccgcgcg ggtggtctcc aaggccgagc tcaccgacca tctctacgag caggacttcg
     3421 atcgcgacag caacgtcatc gaggtcttcg tcggtcgcct gcgcaaaaag ctcgatcccg
     3481 tgaaccccat ccgcaccgtt cgcggccagg ggtaccggtt caacacggaa ggatctgctt
     3541 gaaggcaggc atccagaccc gcctgctcat cacca
//
LOCUS       DBNHKV010000304         6699 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_256354, whole genome shotgun sequence.
ACCESSION   DBNHKV010000304 DBNHKV010000000
VERSION     DBNHKV010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6699
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_256354"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="AC16PP_09530"
     CDS             <1..945
                     /locus_tag="AC16PP_09530"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6481910.1"
                     /translation="LPICDPHHHLWDHPGSRYLLDELLADTGSGHNVVATVFVECASM
                     YRADGPEELRSLGETEFVNGVAAMSASGDYGPARVCAGIVGYVDLTLGSSAGPVLDAH
                     MAASDRFKGIRHAAGWDPSEKVRNSHTNPSEGLLLDDEFRAGFAELGKRGLSFDAWLY
                     HPQIPQLTDLARAFPHTTIIFDHFGGPLGIGPYEGRQDQIFAAWQRDVAALAECHNVY
                     AKLGGLVMPVNGFGFHKRERPATSDEIVAATGRYYRHAIDCFGVDRCMFESNFPVDKQ
                     SCSYHVLWNAFKKMVADGSPAEKASLFHDTAARAYRLT"
     gene            942..1439
                     /locus_tag="AC16PP_09535"
     CDS             942..1439
                     /locus_tag="AC16PP_09535"
                     /inference="COORDINATES: protein motif:HMM:NF016001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR6481911.1"
                     /translation="MTIRDAVWRGFSLFVSLKPVTVFFMNLATWVDRPLMRATSGRIR
                     LSFVIPTLLLRCTGARSGLLREVPLLYVPEGDGALLVGSNGGKPYDPAWCHNLRAQRR
                     VSCLLAGREGTFRVEELSGADRDAAWESAVAIYPGYRRYEQRAGRVIPLFRLIPEADG
                     RARQT"
     gene            1461..1898
                     /locus_tag="AC16PP_09540"
     CDS             1461..1898
                     /locus_tag="AC16PP_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HIR6481912.1"
                     /translation="MNGLRSIALALALVTSAAVLADGIRSPAPEGASLYFIAPADGAV
                     VSGPFTVKFGLKGMGVAPAGVDAPNTGHHHLLINLEEAVNPEVPLPATDQVRHFGGGQ
                     TETQLSLPAGTYRLQLMLGNYLHVPHDPPVMSELITVTVADQP"
     gene            1921..2571
                     /locus_tag="AC16PP_09545"
     CDS             1921..2571
                     /locus_tag="AC16PP_09545"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF036807.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR6481913.1"
                     /translation="MTLKVIGAGFGRTGTLSLKLALERLGLEKCYHMLEVMQNEGHAA
                     VWRAAARGESVGWDGLFAGYQASVDWPSCNFWEAQLQHFADAKVILTRRDPERWYASV
                     MNTIYPSSVARREQADPAIREHMDMVFEVIWDGLFDGRMDDKEHVIAIYEAHGQHVID
                     ALPPEKLLVFEASAGWGPLCDFLDLPVPDEPYPWVNSTEDFRSRVAAASSKPQRDG"
     gene            complement(2568..3230)
                     /locus_tag="AC16PP_09550"
     CDS             complement(2568..3230)
                     /locus_tag="AC16PP_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012455.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HIR6481914.1"
                     /translation="MARRLAGEFMGTLLLLATVVGSGIMGEQLSGGNAAIALLGNTIA
                     TGAILVVIILMFAPVSGAHFNPAVTLTFLLRREIPAGAAAAYCVTQVAAGLAGVWLAH
                     GMFGLEIVQTSENSRSGFGQWLAEGVATFGLVATILAVLRSRPDLVAFAVGLYISAGY
                     WFTASTSFANPAVTIARAFTDTFSGIQPDHVPAFIAAQLVGAALATWTFGWLLSEGER
                     TN"
     gene            complement(3230..3529)
                     /locus_tag="AC16PP_09555"
     CDS             complement(3230..3529)
                     /locus_tag="AC16PP_09555"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6481915.1"
                     /translation="MKLDAASACLESLGNSTRLAIYRALVRAGPAGLPVGKLQARLDI
                     PGSTLSHHIAHLVKNDLVSQTREGRVLRCTANYRTMNDLITYLSDECCRDTPRTT"
     gene            3672..4130
                     /locus_tag="AC16PP_09560"
     CDS             3672..4130
                     /locus_tag="AC16PP_09560"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HIR6481916.1"
                     /translation="MRLQLALNVADIDEAVSYYSRILGVAANKREPGYANFVIEDPAL
                     KLVLFEAPGADERLNHLGFEAFADEDIEAVAGRLEDARVEHEVQTEEVCCFAKQNKVV
                     SYDPQGMMWEWYRVVEDSPTFFAEPEALACCAPAEPEAQGCCPPAERSAG"
     gene            complement(4133..4927)
                     /locus_tag="AC16PP_09565"
     CDS             complement(4133..4927)
                     /locus_tag="AC16PP_09565"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR6481917.1"
                     /translation="MPESLRVMVVCAHPDDAEFHAGGLLVQLAAAGTDIHLLTLTDGS
                     AGHAELDRASLARRRAREAQAAARRLGARCTVWDTPDGELEPSLQLRGRLIVAVREFA
                     PHVLITHRSGDYHPDHRAAAQLVQDASFLLRVPNVQPDAPIMNEDPVILGFCDFFRRP
                     SPFRSDLLVDTEEQLEAVVELLACHESQVYEWLPHIMGLEVGENRRSWLREFYTQRPA
                     AVAHAHADDGARYAEAFELSEYGRQVTPQELAGLLAAGSAKANHSG"
     gene            5094..5276
                     /locus_tag="AC16PP_09570"
     CDS             5094..5276
                     /locus_tag="AC16PP_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481918.1"
                     /translation="MYTVVDIASLQDYVLVEARRKAQEAGTVPIYRKFPVREVLDTLV
                     VDEEIVLFKPSLFPAL"
     gene            complement(5286..>6699)
                     /locus_tag="AC16PP_09575"
     CDS             complement(5286..>6699)
                     /locus_tag="AC16PP_09575"
                     /inference="COORDINATES: protein motif:HMM:NF005686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6481919.1"
                     /translation="AALDTAGMIRRGDASAVEVMDAHYDRIEILNPQINALVNVLPRE
                     AALELARQADRARSRGEPCGPLHGLPMAVKDTHDAAGFPTTWGFVPWAERVARHDGAV
                     AARQRAAGALFIAKSNVPEFALGSHTFNNLFGTTSNPYDLSRTAGGSSGGAAAALATG
                     MLPLADGSDMGGSLRNPAGYCNVVGLRPSLGRVPADGPFGWFARLLTPGPMARNVPDA
                     ALLLSVQAGPNQDDPLSRPEPGRHFRDLPHADPATLKIAWSADLGFAPVDAAMVRLLE
                     AAAVVFEDLGCEVENACPDLRGGMSVFQTQRAASLAQTGRALDRSLPRWREHAKETMI
                     WNVEQGYALTAEDLLRCEVERTAIYQRVGAFFAHHDALLLPSAQVPPFDKSLDWVREI
                     DGRAMETYIDWMTICCAVTVTDLPAISVPGGFTDDGLPLGLQIVGRPHGDLDLLAIAR
                     LFEEATGHGQRRPPQSEHAG"
BASE COUNT         1145 a         2101 c         2277 g         1176 t
ORIGIN      
        1 ctgcccatct gcgatcccca ccatcacctg tgggaccatc ctgggagccg gtaccttctc
       61 gacgagctgc ttgccgacac gggcagcggg cacaacgtgg tagctacggt tttcgtggag
      121 tgtgccagca tgtatcgagc cgacggaccc gaggagctga gatccctggg tgaaacggag
      181 ttcgtcaacg gcgtcgcggc catgtcagca agcggcgatt acgggcccgc ccgggtgtgt
      241 gccggtatcg tcggctacgt agacctgacc ctcggatcct ccgcgggccc cgtgctcgac
      301 gcccacatgg cggcgagcga ccggttcaag gggattcgtc acgccgccgg ctgggatccc
      361 agcgagaagg ttcgcaactc ccacaccaac ccgtccgagg gtttgttgct cgacgacgaa
      421 ttccgggcgg gttttgcgga gctcggcaag cgcggattga gcttcgacgc ctggctctac
      481 cacccacaga tcccccagct caccgacctg gcgcgagctt tcccgcacac gacgatcatc
      541 ttcgatcact tcggcgggcc cctcggcatc ggcccctacg aaggccggca ggaccagatt
      601 ttcgccgcct ggcagcgcga cgtggctgcg ctggccgagt gtcacaacgt ctacgccaag
      661 ctcggtggcc tggtcatgcc cgtcaacggc ttcgggtttc acaagcgtga acggcccgct
      721 acctccgatg agatcgtggc ggcgaccggc cgctactacc ggcatgccat cgactgcttc
      781 ggcgtggaca ggtgcatgtt cgaaagcaac ttcccggtgg acaagcagag ctgctcctat
      841 cacgtgctct ggaatgcctt caagaagatg gtggccgatg ggtctccggc ggagaaggct
      901 tcgctctttc acgacacggc cgctcgagcc tatcgcctga cgtgaccatt cgggacgccg
      961 tctggcgggg attttccctc ttcgtgtcgc tcaagccggt gaccgtattc ttcatgaatc
     1021 tcgccacctg ggtcgacaga cccctcatgc gggcaacttc cggacgcatc cgcctcagct
     1081 tcgtgatccc gacgttgctg ttgcggtgca cgggggcgcg cagcggcctg ctcagggagg
     1141 tgccgctgct ctacgttccc gagggcgacg gggcgctgct cgtgggttcc aacggcggaa
     1201 agccgtatga tccggcctgg tgtcacaatc tccgtgccca gcgccgggtc agctgcctgc
     1261 tcgccggccg cgagggcacc ttccgggtgg aggagctcag cggggcggat cgagacgcgg
     1321 cctgggagtc ggcggtcgcc atctacccgg gataccgacg ctacgagcag cgggcaggac
     1381 gggtcatccc gttgttccgc ctgatcccgg aggccgacgg gcgggcacgg caaacctgaa
     1441 aacatcgacg gaggatcacg atgaacggct tgagatcaat tgccttggcg ctggctctgg
     1501 tgacgtcggc ggccgtgctg gcggacggca tccgctcgcc ggcaccagaa ggggcgtcgc
     1561 tctatttcat agcccccgcc gatggcgccg tggtgagtgg gccgttcacg gtgaagttcg
     1621 gcctgaaagg catgggcgtc gccccggccg gcgtggacgc gcccaacacc ggccaccacc
     1681 acctcctgat caatctcgag gaagcggtga atcccgaagt gccgctgcct gccacggatc
     1741 aggttcgcca cttcggcggc ggccagacgg aaacacagct cagccttccg gccggaacct
     1801 accgcctgca gctcatgctg ggcaattatc tgcacgtgcc tcacgatccc ccggtgatgt
     1861 ccgagttgat cacggtcacg gtcgccgacc agccgtgagg acaaggtcga ggaactcatc
     1921 gtgacgctga aagtcatcgg cgcgggcttc ggccgaacgg gcacgctctc gctcaagctc
     1981 gccctggaac gcctggggct ggagaagtgt taccacatgc tcgaggtcat gcagaacgag
     2041 ggtcacgcgg ccgtctggcg ggcggcggca cggggcgagt cggtgggctg ggacggcctc
     2101 tttgccggct accaggcttc ggtggactgg ccctcctgca acttctggga agcgcagctt
     2161 cagcactttg ccgacgccaa ggtcatcctc acccgccggg accccgagcg ctggtacgcc
     2221 agcgtcatga acacgatcta tccgtcgagc gttgcccggc gcgagcaggc ggatccggcg
     2281 atccgcgagc acatggacat ggtgttcgag gtgatctggg atggtctgtt cgacgggcgc
     2341 atggatgaca aagagcacgt catcgcgatc tacgaagccc acggccagca cgtcatcgat
     2401 gcgctgccgc cggaaaagct cctcgtcttc gaggcgagtg ccggttgggg accgttgtgc
     2461 gattttcttg atctgccggt gcccgacgag ccatatccct gggtgaacag caccgaggac
     2521 tttcgctcgc gggtggccgc agcaagctcg aagcctcagc gggacggtta gttggtcctt
     2581 tcgccctcgg agagcagcca gccgaacgtc caggttgcga gcgcggctcc gacgagctgg
     2641 gctgcgatga atgcggggac gtggtcgggc tgaattcccg aaaacgtgtc cgtgaacgcc
     2701 cgggcgatgg tcactgcggg gttggcgaag gacgtcgacg cggtgaacca gtagccggcg
     2761 gagatgtaga gccccacggc gaacgctacc aggtcgggac gggagcgcag cacggcgagt
     2821 atggtcgcta ccaggccgaa ggtggcgacc ccctcggcca gccattgccc gaagcctgac
     2881 cgggaattct cggatgtctg aacgatttcc agcccgaaca tcccatgggc cagccacacg
     2941 ccggccagcc cagcggccac ctgggtcacg cagtaggcgg ccgccgcacc cgcggggatc
     3001 tcgcgccgca ggaggaacgt gagcgtgacc gcggggttga agtgggctcc ggagacgggc
     3061 gcgaacatca aaatgatgac caccaggatg gcgccggtgg cgatggtgtt gccgagcagg
     3121 gcgatggccg cgttgccccc ggaaagctgc tctcccatga tccccgatcc cacgacggtg
     3181 gccagcagca gcagggtgcc catgaactcg cctgcaagcc ggcgagccat caggtggtcc
     3241 tcggggtgtc ccgacagcac tcgtcggaca ggtaggtgat cagatcgttc atggtcctgt
     3301 agttcgccgt gcagcgaagc acgcggccct cgcgggtctg ggagaccaga tcgtttttca
     3361 ccaggtgagc gatgtgatgc gaaagggtgg acccgggaat gtccagacga gcctgaagct
     3421 ttccgacggg caagccggcc ggtccggcac gcacgagggc tctgtagatg gcgagccggg
     3481 tgctgttgcc gaggctttcg aggcatgccg aggcggcatc tagtttcatc tggagtctgc
     3541 ttgaatcctt cgggccatcg cagtttacgt tatttccatg aggctagaaa taacgaaacg
     3601 gtattggcgc gggtcaatta tttcgaataa agtggaaata tattttcaaa gtattgatgc
     3661 aggagaatcg catgcgtctt caactggcac tgaacgtcgc agacatagac gaggcggtga
     3721 gctactactc gcggatcctg ggcgtggcgg ccaacaagcg ggagccgggc tatgccaact
     3781 tcgtgattga agacccggcg ctcaagctcg tgctcttcga agcgcccgga gccgacgagc
     3841 ggctgaatca cctgggcttc gaggcctttg ccgacgagga catcgaggcg gtggccggac
     3901 ggctcgagga tgcgcgcgtg gagcacgagg tgcagaccga agaggtctgc tgcttcgcaa
     3961 agcagaacaa agtcgtcagc tacgatccgc agggcatgat gtgggagtgg tatcgggtgg
     4021 tggaggactc cccgactttc ttcgcggagc ccgaggccct ggcgtgctgc gcgccggccg
     4081 agcccgaagc ccagggctgc tgcccccctg ccgagcggtc ggcgggttga agtcagccgc
     4141 tgtggttggc tttggcagag cccgcggcga gcagcccggc cagctcttgt ggcgtgacct
     4201 gtcgcccgta ctctgacagc tcgaaagcct cggcgtagcg ggcaccgtcg tcggcatggg
     4261 catgggcgac ggccgccggc cgctgtgtgt agaactctct cagccagctg cgacggttct
     4321 cccccacctc gaggcccatg atgtgaggca accactcgta aacctgggat tcgtggcacg
     4381 ccagcagctc caccacggct tccagctgct cttccgtgtc caccagaagg tctgaccgga
     4441 agggcgaggg gcgacggaag aagtcgcaga atcccaggat taccggatcc tcgttcatga
     4501 tgggcgcatc gggctggacg ttcggtacgc gcagcaggaa gctggcgtcc tggaccagct
     4561 gggctgctgc tcggtggtcc gggtggtaat cgcccgagcg gtgcgtgatc agcacgtggg
     4621 gagcgaattc gcgcacggcg acgatcagtc gcccgcgcag ctggaggctc ggctcgagct
     4681 cgccgtctgg cgtatcccag acggtgcacc gcgcgccgag ccggcgagcg gcggcctggg
     4741 cttccctggc ccgtcgtcgg gcgagcgagg cgcgatccag ctcggcgtga ccggcggagc
     4801 cgtccgtgag ggtgagcagg tggatgtccg tgcccgctgc ggccagctgg acgagcagcc
     4861 cgccggcgtg aaactccgca tcgtcgggat gggcgcagac gaccatgact cgtaacgatt
     4921 ccggcatggc gtcaggtctc cgttacatct cttacatcct ttttacatca accatttacg
     4981 gcgtggatag actgtgggtg ttgggggttg gacccgcttc gggccgttgt tcgttggttt
     5041 tgagaagcga ctttcgctgt ctcgtcggtc gcgatttttg cgccgatgag gacgtgtaca
     5101 ccgtggtgga catcgcctcg ttgcaggact acgtgctcgt ggaggctcgc cggaaagctc
     5161 aggaagccgg caccgtcccc atctaccgca aatttcccgt ccgggaggtt ctggacacgc
     5221 tcgtggtcga cgaggagatc gtcctcttca agccgagcct gtttcccgcg ctctagggtc
     5281 gtttttcagc cggcatgctc gctctgcggg ggacgccgct ggccatggcc ggtagcctcc
     5341 tcgaacagcc gggcgatggc cagcagatcg aggtcgccgt gaggtcgccc gacgatctgc
     5401 aggcccaggg gcaggccgtc gtccgtgaac ccgccgggga ctgagatcgc ggggagatcg
     5461 gtgacggtca cggcgcagca gatggtcatc cagtcgatgt acgtctccat ggcccggcca
     5521 tcgatctcgc gcacccaatc cagcgacttg tcgaacggtg gtacctgggc agaaggcagc
     5581 agcagcgcgt cgtggtgggc aaagaaagcg ccgacgcgct ggtaaatcgc cgtacgttcg
     5641 acctcgcagc gcaacagatc ctctgcggtg agcgcgtagc cctgctccac gttccagatc
     5701 atggtttcct tggcatgttc gcgccagcgc ggtagcgagc gatccagggc ccggccggtc
     5761 tgggccaggc tggccgcgcg ctgggtctgg aataccgaca tgcctccgcg caggtccgga
     5821 caggcattct ccacctcgca gccgaggtct tcgaaaacga ccgccgctgc ctcgagcagc
     5881 cggaccatgg cggcgtccac gggtgcaaag ccgagatctg cggaccaggc gatcttcagg
     5941 gttgccggat cggcgtgggg cagatcgcgg aagtgtcggc ccggctccgg ccggctcagg
     6001 gggtcgtcct gattggggcc tgcctgaacg gacagcagca gagccgcgtc gggcacgtta
     6061 cgagccatgg ggccgggggt gagcagccga gcaaaccagc cgaacgggcc atccgccggg
     6121 acgcgcccca atgagggccg aaggccgaca acgttgcagt acccggccgg gttgcgcagc
     6181 gatccgccca tgtccgaccc gtcggccagg ggaagcatcc ccgttgccag cgccgcggcg
     6241 gcgccgccac tgctgcctcc ggccgtgcgc gacaggtcgt agggattgct cgtggttccg
     6301 aagaggttat tgaacgtgtg tgatcccagg gcgaactcgg gcacgttgga tttcgcgatg
     6361 aaaagtgcgc ctgcggcccg ttggcgtgcg gccaccgctc catcgtgcct agccacgcgt
     6421 tcggcccagg ggacaaatcc ccaggtggtc ggaaagcctg cggcgtcatg ggtgtccttg
     6481 acggccatgg gcagaccgtg cagaggaccg cacggctcac cgcggctgcg cgcccgatcc
     6541 gcttggcgag ccagctcgag tgccgcctct ctgggcagaa cgttgacgag cgcattgatc
     6601 tgggggttca ggatttcgat gcgatcgtag tgtgcgtcca tgacttccac ggcggatgcg
     6661 tctccccgtc gaatcatccc ggcagtatca agagccgct
//
LOCUS       DBNHKV010000305         4112 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1248430, whole genome shotgun sequence.
ACCESSION   DBNHKV010000305 DBNHKV010000000
VERSION     DBNHKV010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1248430"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..855
                     /locus_tag="AC16PP_09580"
     CDS             109..855
                     /locus_tag="AC16PP_09580"
                     /inference="COORDINATES: protein motif:HMM:NF018038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR6481920.1"
                     /translation="MSGPINAYLVANARYHDTDFAALELLKLLAEDDNIQTKVADSFA
                     NVEAIAASDFLITYTCDLRPTHEEEVGLREFVSNGGRWIALHGTNALLDFTDEGQVIA
                     PRINDTFMETLGSRFISHPAIEPYTVTVSNPDHPMVRGIEPFEATDEIYLCEYYGKVE
                     PLLETRYTGTFEAGYVENEWPDDDPRLVAYTHRVGEGEVLYITLGHCCGKYDMQPIVE
                     EAPVVRGSWDNPIFYELLRRAIRWAKDELN"
     gene            complement(897..1286)
                     /locus_tag="AC16PP_09585"
     CDS             complement(897..1286)
                     /locus_tag="AC16PP_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR6481921.1"
                     /translation="MIYLDTSVALAHLLAEDRRPPDWIWQETLVASRLIEYELWNRLH
                     ALDFAASHGEAARGLVSQIALLELSPEVLQRALEPYPVRIRTLDALHLASLGFLQTHG
                     QQVELATYDTKMADAARAMRMSLIDLP"
     gene            complement(1283..1576)
                     /locus_tag="AC16PP_09590"
     CDS             complement(1283..1576)
                     /locus_tag="AC16PP_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR6481922.1"
                     /translation="MKAVGIKVLKNKLSEYVRLAAGGETVLVTDRDRVVAQITAPGPA
                     VSPLLADAALADAVRKGHIRAPLLRTGQPPEAPPPALKLSDVLEGLEQDRGER"
     gene            complement(1773..3164)
                     /locus_tag="AC16PP_09595"
     CDS             complement(1773..3164)
                     /locus_tag="AC16PP_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6481923.1"
                     /translation="MVKHASTDAAGRDAVPPLSRRTLFYYGLADMPIQMAAVPVAAFV
                     PNYYGQDLGVSLAAVGLVLLLSRSFDAITDPLIGWLSDRTDTRWGRRRVWMLASVPIL
                     MLAIYKLFMPDPGDASAVYLLIWLVVLWLGWTMLFIPYYAWAAELSPEYNERSRITGW
                     RSSIGLAANVLSKLVPVVAIFCCAYGGTQETLFLVGTMTLILLPLTVGLTVAMVPEGK
                     DFIPARMPLVPGLKIMWRNGPFKRLVAAFFVNQLGTSISTALIVFYVRSVLQDDDKTI
                     LQLLVYYGFNLCGIPFFVRYSGIVGKHRAWCIALLLFAFFMLGYLLLGAGDFYWMLPI
                     TAITGFLAGSFWVIPNSMKADVIDIDRLESGEDRTAWYFAVWSLATKVALSVGPAIAL
                     GLLSLTGYVPAAEAGAYEGSTLGLKLLFVFGPSTGLLLTALIAWRYPLTETKHRELRQ
                     ALARSRSRPRPAG"
     gene            complement(3148..>4112)
                     /locus_tag="AC16PP_09600"
     CDS             complement(3148..>4112)
                     /locus_tag="AC16PP_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6481924.1"
                     /translation="YAWAAELSPDYNERTRITGWRSWIGMAANVVSKVVPVIAIYCCA
                     FGGTEETLFLIGTMTLILLPVTVGLTVMKVPEGRDYLPTRIPLARGLKLMWQNAPFKR
                     LVLAFFINQLGSSISTALVVFYIRVVLQEDAMPIVFLLTYYLFNLSGIPLWVWFSGKV
                     GKHRAWCVGLALFGTAQCGYLLLGPGDFLYMLPIGALTGFLGGSFWVLPNSMKADVID
                     VDTLNTGEDRAAWYFAVWSFTTKVGQSIGPWLALTLLAFVGYDPASQPEPTAGGLLGL
                     KLLYVFGPACGFWLAALIAWNHPLTESRHREIRASLEKVRGQAR"
BASE COUNT          813 a         1302 c         1367 g          630 t
ORIGIN      
        1 cctggctcgc cacagctttc ggcggtggca tcggggcagg cgcatctggt cgttgcgaaa
       61 gaccccggga tttctgtacc gtgggctcgg aacacgggga gatgaacgat gtccggaccc
      121 atcaacgcct atcttgtcgc caacgcgcgc taccacgaca cggacttcgc tgctctggag
      181 cttttaaagc tcctggccga ggacgacaac atccagacca aggtggccga cagctttgcc
      241 aacgtcgagg ccatcgccgc gtcggatttc ctgatcacct acacctgtga cctgcgcccg
      301 actcatgagg aagaagtggg gctgcgggaa ttcgtgagca acggcggccg ctggatcgcg
      361 ctgcacggca cgaatgccct gctcgacttc acggacgaag gccaggtgat cgccccgcgc
      421 atcaacgaca ccttcatgga gaccctgggc agccgcttca tctcgcaccc cgccatcgag
      481 ccctacaccg tgacggtctc caaccccgac caccccatgg tccggggcat cgagccgttc
      541 gaggctaccg acgagatcta tctgtgcgag tactacggca aggtggagcc gctgctggag
      601 acgcgctaca cgggcacgtt cgaggccggc tacgtggaga acgagtggcc cgacgacgat
      661 ccccggctgg tggcgtacac ccatcgggtg ggcgaagggg aggtgctcta catcaccctc
      721 ggccactgct gcggcaagta cgacatgcag ccgatcgtgg aggaagcccc cgtggtcagg
      781 ggatcctggg acaaccccat cttctacgag ctgctgcgcc gcgccatccg ctgggccaag
      841 gacgagctga actagcggcg tagccgccga agcctctctt tcgctgctga gggcgtttac
      901 ggcaggtcga tgaggctcat ccgcatggcc cgcgcggcgt cggccatttt cgtgtcataa
      961 gtcgccagtt ccacctgctg gccgtgggtc tgcaggaacc ccagcgaggc gaggtgcaaa
     1021 gcatccagtg tccggatcct gaccgggtac ggctccaacg cccgttgcag cacctcgggc
     1081 gagagttcga gcaacgcgat ttgcgacacc agccccctgg cggcttcgcc gtgtgatgct
     1141 gcgaaatcca aggcgtgcag ccggttccac agctcgtact caatgagacg gctggccacc
     1201 agggtctcct gccagatcca gtcagggggg cggcgatcct cggcgagcag gtgggccagg
     1261 gcgacggacg tgtccaggta aatcaccgct cgccgcgatc ttgctcgagg ccttccaaaa
     1321 cgtcgctcag ctttagcgcc gggggaggcg cctccggcgg ctggccggtg cgcagaagcg
     1381 gggcgcggat gtggcctttt cgaactgcgt ccgccagggc ggcgtctgcg agcaatgggc
     1441 tgactgccgg ccctggggcg gtgatctgcg ccacgacgcg atcgcgatcg gtcaccagaa
     1501 cggtttcgcc gcccgctgcc aggcgcacgt actcgctcag cttgttcttg agtactttga
     1561 tgcctacggc cttcatttca caaaagtagc tactagtagc taccaatgca accgggattt
     1621 tcgtctgccc gaaaaaacta ctcgagcgcg gctccctgcg gaaccggcag cgcttgtacg
     1681 agtatgtagt ctgtcgccgg aggcgagcgg cagccgaagc cgcctctagc ctctccaccg
     1741 ctggacgggg cctttacagg agcgcgctgg gttcaacccg ccggccgcgg cctgctgcgc
     1801 gagcgggcga gagcctgtct cagttcccgg tgcttggtct cggtgagcgg gtagcgccac
     1861 gcgatcagcg cggtgaggag gagcccggtg gacggcccga agacgaacag cagcttgagc
     1921 cccagcgtcg agccttcgta agcgcctgcc tcggcagccg gcacgtagcc ggtgagggag
     1981 agcagcccca gagcaatagc cggcccgact gacagggcga ccttggtggc cagggaccag
     2041 acggcgaagt accaggcggt gcggtcctcg ccgctctcca gccggtcgat gtcgatcacg
     2101 tcggccttca tggagttggg gatcacccag aaagacccgg cgagaaaacc cgtgatcgcc
     2161 gtgatgggca gcatccagta gaagtcgccg gctcccagca gcaggtagcc cagcatgaag
     2221 aaggcgaaca gcaacagagc gatgcaccag gctcgatgct tgccgacgat gccggagtag
     2281 cgcacgaaaa acgggatgcc gcagaggttg aacccgtagt acaccagcag ctgcaggatg
     2341 gtcttgtcgt cgtcctgcag cacgctgcgc acgtagaaga cgatgagcgc cgtggagatg
     2401 gaggtcccca gctggttgac gaagaacgcg gcgaccagac gtttgaaggg cccattgcgc
     2461 cacatgatct tcagccccgg caccaggggc atgcgggccg ggatgaagtc cttgccttcg
     2521 ggaaccatgg ccaccgtcag ccccacggtc agtggcaaaa gaatcagcgt catggtgccg
     2581 acgaggaaca gggtctcctg ggtgcccccg taggcacagc agaagatggc caccacgggt
     2641 accagcttgc tgagcacgtt ggcggcgagc ccgatgctgg accgccagcc cgtgatgcgg
     2701 gaacgctcgt tgtactccgg agacagctcg gccgcccagg cgtagtaggg aatgaacagc
     2761 attgtccagc cgagccacag aaccaccagc cagatcagca ggtagacggc ggaggcgtcg
     2821 ccgggatcag gcatgaagag cttgtagatg gcgagcatca ggataggcac ggaagccagc
     2881 atccagaccc ggcgacggcc ccagcgcgta tccgtgcggt ccgacagcca gccgatcagc
     2941 ggatcggtga tggcgtcgaa cgatcgcgag agcaggagca ccagcccgac ggccgccagg
     3001 ctcacgccca gatcctggcc gtagtagttg ggcacgaagg cggccaccgg caccgcggcc
     3061 atctggatgg gcatgtcggc caggccgtag tagaacagcg tacgccgcga aagcggcggc
     3121 acggcgtccc ggccggctgc gtcggtgcta gcgtgcttga ccacggacct tctccaggct
     3181 ggcgcgtatc tcccggtggc gcgactcggt gagcggatga ttccaggcaa tgagggcagc
     3241 cagccagaag ccgcaggccg gcccgaacac gtaaagcagc ttgagtccca gcaggccacc
     3301 cgccgtgggc tccggctggc tggccggatc gtagcccacg aaggccagaa gcgtcagcgc
     3361 gagccagggg ccgatggatt gcccgacctt ggtggtaaag gaccagacgg cgaagtacca
     3421 cgccgcgcgg tcctcgccgg tgttgagcgt gtccacgtcg atcacgtccg ccttcatgga
     3481 gttcgggagc acccagaaag agccaccaag aaacccggtg agcgcgccga tgggaagcat
     3541 gtagaggaag tcgccgggac ccagcagaag atagccgcat tgggcagtgc cgaacagggc
     3601 caggccgacg caccaggcgc gatgcttgcc caccttgccc gagaaccaga cccacagcgg
     3661 aatgccgctc aagttgaaca ggtagtaggt gagcagaaac acgattggca tggcgtcctc
     3721 ctgcaacacc acgcgaatgt agaacaccac cagcgccgtg gagatggacg agcccagctg
     3781 gttgatgaag aacgccagca ccaggcgctt gaacggagcg ttctgccaca tgagcttgag
     3841 cccccgcgcc aggggaatac gggtgggcag gtagtcgcga ccttcgggga ccttcatcac
     3901 ggtcaggccc acggtgacgg gcagcaggat cagggtcatg gtgccgatga gaaacagcgt
     3961 ctcctcggtg ccgccgaacg cgcagcagta gatggcgatg acgggaacca ccttgctgac
     4021 cacgttggcc gccatgccga tccaggaacg ccagcccgtg atgcgggtgc gctcgttgta
     4081 gtccggagac agctcggccg cccaggcgta gt
//
LOCUS       DBNHKV010000306         4286 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1603008, whole genome shotgun sequence.
ACCESSION   DBNHKV010000306 DBNHKV010000000
VERSION     DBNHKV010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1603008"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="AC16PP_09605"
     CDS             complement(<1..847)
                     /locus_tag="AC16PP_09605"
                     /inference="COORDINATES: protein motif:HMM:NF014826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR6481925.1"
                     /translation="MTAVTPASTEGFVHLRVHSEFSVADGLMKVGALADAARRLNMPA
                     VALTDRSNLFGLVKFYQACLSAGVKPLVGADLVYQTDEGGRFRIAVLAMNSQGYRNLL
                     ALVSAAYLDTDERGVLSAGQIFARQEGLLVLSGGRQGEVGQALAKGDLPGAQRIAALW
                     EERFPERFYLELVRTGRGGEDAYIDGAVRLAAELELPVVATNDVCFLAADDFEAHETR
                     VCIYDGRTLDDPRRERSYSSDQYLRTPAEMTALFSDLPEAIENTVQIARRCNVQIELG
                     DYHLPN"
     gene            complement(844..1464)
                     /gene="rnhB"
                     /locus_tag="AC16PP_09610"
     CDS             complement(844..1464)
                     /gene="rnhB"
                     /locus_tag="AC16PP_09610"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008566891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR6481926.1"
                     /translation="MGLFDDLAGTNLTAGVDEVGRGPLAGPVVAGAVVLDPLRPIDGL
                     KDSKRLNARRRAELAEEIRAKALAWGLGRAEVEEIDALNILRASHLAMQRAVAALSVE
                     PEMILVDGNLLPVFSVPAVAVVKGDARVPEISAGAILAKVARDREMIELDRLYPGYGF
                     AGHKGYPTAEHLSALAELGACAVHRTSFAPVRDLKLRRPTVERLPL"
     gene            complement(1464..2681)
                     /gene="lpxB"
                     /locus_tag="AC16PP_09615"
     CDS             complement(1464..2681)
                     /gene="lpxB"
                     /locus_tag="AC16PP_09615"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HIR6481927.1"
                     /translation="MSWSAGAADTDPAAPRGDICLGILAGEASGDSLGAGLMAALKRR
                     YPRARFVGIGGPRMLAQDLDPVAPMERLQVNGLVDPVKRLPDLVRLLRQLVRCYEASR
                     PAVFIGVDFNVFNFLLERMLKARGIPTVHYVSPSVYAWRRGRVGRIARSADLLLTLFP
                     FEPALYDDAGLEAVFVGHPRADAIDIDAGGAEGRARARRELQIPPDRFCMAVLPGSRL
                     SEVRLMGAPFLDAAGIVARRLPGTLVIVPCLRPEIRTLLEALLAARPELDAVLYDGDA
                     ELALTASDGALVKSGTSTLEAMLLRRPMVVSYKLGPLTYRLIRLLVRTPHIALPNILA
                     GEELVPEVLQDEATPEALAEKFLDQLDKARRDPEHLARFSDLHRQLRMNADERAADAV
                     SRLLDGRAAHTEA"
     gene            complement(2678..3478)
                     /gene="lpxA"
                     /locus_tag="AC16PP_09620"
     CDS             complement(2678..3478)
                     /gene="lpxA"
                     /locus_tag="AC16PP_09620"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIR6481928.1"
                     /translation="MIDPRAIIDPSAVIGEGVTIGPWTFIGPDVVIGDGCWVAPHVII
                     RGPTRIGRNNKIYQFATVGEDTPAMAYHGEPTTLEIGDDNVIREGVTIHRGMMQDLGR
                     TVIGSHCLLMAYVHVGHDCIVGDHVIMANNSSISGHVRVGDHANFGGYSGVPQFRRIG
                     AYSHVSGMSLVLKDVPAYMTVMGNPASAIGLNIEGMRRRGYSKELIDALRSAHRVVYR
                     EGRTIQDACRRLEPAARDWPEVELFLESIRDSRWGIVRPRGRGGETRN"
     gene            complement(3480..3917)
                     /gene="fabZ"
                     /locus_tag="AC16PP_09625"
     CDS             complement(3480..3917)
                     /gene="fabZ"
                     /locus_tag="AC16PP_09625"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR6481929.1"
                     /translation="MKDITEIFDYLPHRYPLLLVDRVLEIVPGERIRGFKNVTFNEDF
                     FNGHFPGNPVMPGVLIVEAMAQLSGVLAFETHDRRPSDGYLYYLASIDNTRFKRPVVP
                     GDRLMMESELVADRRGLMKFKLAAFVDDDLACTAEIACMERKL"
     gene            complement(3978..>4286)
                     /locus_tag="AC16PP_09630"
     CDS             complement(3978..>4286)
                     /locus_tag="AC16PP_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR6481930.1"
                     /translation="IGAHTVICGCVGIAGSTRIGRHCVLAGKCGIGGGGPVELADGVV
                     LSGGTNVMVSIDRPGVYSGSVLHETHPRWKRNAMRLRRLDDLFRRVADLEKKVKDQTG
                     "
BASE COUNT          779 a         1442 c         1348 g          717 t
ORIGIN      
        1 agttgggcag gtgatagtcg ccgagctcga tctgaacgtt gcagcggcgg gcaatctgaa
       61 cggtgttctc gatcgcttca ggcaggtcgc tgaagagagc cgtcatctcc gcgggcgttc
      121 gcagatactg gtcggacgag tagctgcgtt cacgacgcgg atcgtccagc gttcggccat
      181 cgtagatgca gaccctcgtc tcgtgagcct cgaagtcgtc cgcggccaga aagcagacgt
      241 cattggtagc taccacgggc agctcgagtt ccgccgccag ccgcaccgcc ccatcaatgt
      301 aagcatcctc gccgccgcga ccggtgcgga cgagctccag gtagaagcgt tcgggaaagc
      361 gctcctccca cagggccgct atcctttgag ccccagggag gtcgcccttg gcaagagcct
      421 ggcccacctc accctggcgc ccgccggaaa gcaccagcag cccctcctgc cgggcgaata
      481 tctggccggc gctgagcacg ccgcgttcgt cggtatcgag gtaggcggcg gaaacgaggg
      541 ccagcaagtt gcgatatccc tgggagttca tggccagaac ggctatccgg aaccggccgc
      601 cttcatccgt ctggtagacg agatccgcgc ccaccaacgg cttcacgccc gcgctgagac
      661 aggcctggta gaacttcacc agcccgaaca ggttgctccg gtcggtcagg gcgactgccg
      721 gcatgttcaa gcgccgcgcg gcatcagcca gggcgcccac tttcatcagg ccatcggcga
      781 ccgagaactc gctgtgaacc cgcaggtgga cgaagccttc agtgcttgcc ggggttacgg
      841 cggtcacagc ggaagtcgct ccacggtcgg ccgacggagc ttgagatcgc ggaccggcgc
      901 aaagctcgtc cggtggacgg cacacgcacc cagctccgcg agcgccgaga ggtgctcggc
      961 ggtcggatag cccttgtggc cggcaaagcc gtaacccgga taaagacgat ccagttcgat
     1021 catttcccga tcacgcgcca ccttggccag gattgctccg gcgctgatct ccgggacgcg
     1081 ggcgtcccct ttgaccaccg cgacggcggg aaccgagaag accggcagca ggtttccatc
     1141 gacgaggatc atctccggct ccacggaaag agccgctacc gcccgctgca tcgcaagatg
     1201 gctggcccgg agtatgttga gcgcatcgat ctcctcgacc tccgcgcgtc cgagccccca
     1261 cgcaagggcc ttagcccgga tttcctcagc cagctcggcc cgccgcctgg cgttcagccg
     1321 cttggagtcc ttgaggccgt caatgggcct cagcggatcg agcaccacgg cgccagccac
     1381 gaccggaccg gcgagtggcc cacgccctac ctcgtctacg cccgcggtga ggttagtacc
     1441 ggcgagatcg tcgaatagtc ccatcaagcc tcggtatgcg cggcgcggcc gtccagcagc
     1501 cggctcacgg catctgccgc cctttcatcg gcgttcattc tgagttgtcg gtgcaggtct
     1561 gagaaccggg ctaaatgttc agggtcgcgt ctggctttgt ccagttgatc gaggaatttt
     1621 tccgcgagcg cctcgggggt ggcctcatcc tgaagcactt ccggcaccag ttcctcccct
     1681 gcaaggatgt tcggcagcgc aatatgggga gttctcacca gcagccggat caaccgatag
     1741 gtcaaggggc caagcttgta gctcaccacc attggccggc gcagcagcat ggcctccagc
     1801 gtgctggtgc cggacttgac cagcgcgccg tccgaggccg tcagtgccag ctcggcatcg
     1861 ccgtcgtaga gcaccgcgtc cagttcgggc cgggcggcca gcaatgcctc cagcaatgtg
     1921 cggatctccg ggcggaggca cggcacgatc accagggtgc cgggcagacg ccgggcgacg
     1981 atgcctgccg cgtcgagaaa cggcgcgccc atgagccgga cctcggagag ccggctgccg
     2041 ggcagaaccg ccatgcagaa ccggtcagga gggatctgga gttcgcgacg agcccgcgcc
     2101 cgcccttcgg ccccccccgc gtcgatgtcg atggcgtctg cccggggatg gcccacgaac
     2161 accgcctcga gaccggcatc gtcatacaac gccggctcga acggaaacag ggtgaggagc
     2221 agatcggcag atcgggcgat gcgcccaacg cgcccgcggc gccaggcgta aaccgagggg
     2281 ctgacatagt gcacggtcgg gatgcctcga gccttgagca tccgctccag cagaaagttg
     2341 aacacgttga agtcgacgcc gatgaacaca gccggacggg aggcttcgta gcaacgcacg
     2401 agctgccgca gaaggcggac cagatcagga aggcgcttta cggggtcgac cagcccgttg
     2461 acctgcaggc gctccatggg cgcgaccgga tctagatcct gcgccagcat ccgcgggcca
     2521 ccgatgccga cgaaacgagc gcgcgggtag cgtctcttga gcgcggccat gagccccgct
     2581 ccgaggctgt ccccggatgc ctctccggcg agaataccca gacagatgtc gccgcgcggc
     2641 gccgccggat cggtgtcggc agctcccgcc gaccagctca atttcgggtt tcgccgccgc
     2701 gaccccgggg acggacgatg ccccatcgcg agtcccgaat ggattccagg aacaactcga
     2761 cctcgggcca gtcccgggcc gccggctcca ggcgtcgaca ggcatcctga atggtcctgc
     2821 cctcgcgata gacgaccctg tgcgcgctgc gaagcgcgtc gatgagctcc ttggagtaac
     2881 cgcgccgccg catcccttcg atgttcaggc caatggcaga ggccggattg cccatcaccg
     2941 tcatgtaggc cggcacgtcc ttcagcacca ggctcatgcc tgaaacgtgg gagtaggcgc
     3001 cgatccggcg aaactgcggg acgcccgagt aaccgccgaa attggcatga tctcctaccc
     3061 tcacatgccc ggagatggac gagttgttcg ccatgatcac gtgatcgccc acgatgcagt
     3121 catggcccac gtgcacgtat gccatgagca gacaatggct gccgatcacg gttctgccga
     3181 gatcctgcat catgccgcga tgaatggtga cgccctcgcg gatcacgttg tcgtcgccga
     3241 tctccagggt ggtgggctcc ccgtgatacg ccatggccgg cgtgtcctcg cccaccgtgg
     3301 cgaactggta aatcttgttg ttgcggccga ttcgggttgg gcccctgatg atcacatggg
     3361 gcgcgaccca gcagccgtcg ccgatcacca cgtcggggcc gatgaacgtc cacggaccga
     3421 tagtgacgcc ttccccgatg accgcgctcg ggtcgatgat ggcgcgcggg tcgatcacct
     3481 cagagcttcc gttccatgca ggcgatctcc gcagtgcagg caagatcgtc gtccacgaac
     3541 gccgcgagct tgaatttcat cagcccccgc cggtctgcca ccagctcgct ctccatcatc
     3601 aggcggtcac cgggcaccac cggacgcttg aatcgggtgt tgtcgatgct cgccagatag
     3661 tagaggtagc catccgaagg gcgccggtca tgggtctcga aggccagcac gcccgagagc
     3721 tgcgccatgg cttcgacgat gagaacgccc ggcatcacgg ggttgcctgg aaagtgaccg
     3781 ttgaagaagt cttcgttgaa ggtcacgttc ttgaatcccc gaatccgctc ccccgggacg
     3841 atttccagca cgcggtccac gagaagcagc gggtagcgat gggggagata gtcgaagatt
     3901 tcggtgatgt ctttcatgcc gatgaccacg ttctttgtcg ggtttgcgct gatcgacccg
     3961 aaagccaaaa ctgccgatca gcctgtctgg tcctttacct tcttctcgag gtccgccact
     4021 cgccggaaca gatcgtccag ccggcgcagc cgcatggcgt tccgcttcca gcgcggatgg
     4081 gtctcgtgca gcaccgagcc cgaatacaca ccgggccgat cgatggaaac catcacgttg
     4141 gtcccgccac tgagcactac cccatcggcc agctccacag gcccgcctcc gccgatgccg
     4201 catttgccgg cgagcacaca gtgtctgccg attcgtgtgc ttcccgcaat ccccacgcac
     4261 ccgcagatca cggtgtgggc gccgat
//
LOCUS       DBNHKV010000307         3532 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_256541, whole genome shotgun sequence.
ACCESSION   DBNHKV010000307 DBNHKV010000000
VERSION     DBNHKV010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_256541"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="AC16PP_09635"
     CDS             complement(<1..946)
                     /locus_tag="AC16PP_09635"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HIR6481931.1"
                     /translation="MSESADPQPSSGRRTGDPWPPVRSGVGAWGRDHARIARETVHFV
                     SARMGTSLLVWLLVGIALALPAGLYLIERNLAGMTAAWEGRPGLTAYFELGASTAQIQ
                     SAVEALKSDARVTDVSVTNPEEALAEFRRYTELADALDLLERNPLPASLKATLVPQTD
                     AAALDPLIEQVRKQAGVAEVVLEKTWLERVSDISSVVSRLGIILGLLFGTGALLVTAT
                     SVRLAIEARLEELKVLKLIGATERQMRRPFLYFGAFYGVGGGLVAAMLISLCLLIVEA
                     PLSNLFGSYGEELDLVGFDAAFLGGLLGIGAILGVLGAL"
     gene            complement(939..1649)
                     /gene="ftsE"
                     /locus_tag="AC16PP_09640"
     CDS             complement(939..1649)
                     /gene="ftsE"
                     /locus_tag="AC16PP_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR6481932.1"
                     /translation="MAQIEFHGVTKRFDSGQEALVNVDVAFAEGSMAFLTGHSGAGKS
                     TFLKLLLCLDQPSRGQILVNGVNVTRMRGRRLAHYRQHLGAVFQDHHLLSHRSVYDNV
                     SLPLRITGMKERDIARRVRAALSRVGLLEKERLFPRYLSTGEQQRVGIARAVVNRPKI
                     LLADEPTGNLDPELSRDIMNLFELFNQVGTTVIVASHDRDLIESMGVRIIELDQGAVV
                     RDGVHARLVDPDDEVGNV"
     gene            complement(1705..2679)
                     /gene="ftsY"
                     /locus_tag="AC16PP_09645"
                     /pseudo
     CDS             complement(1705..2679)
                     /gene="ftsY"
                     /locus_tag="AC16PP_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
     gene            2716..3309
                     /gene="rsmD"
                     /locus_tag="AC16PP_09650"
     CDS             2716..3309
                     /gene="rsmD"
                     /locus_tag="AC16PP_09650"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR6481933.1"
                     /translation="MQFRRLRLRAGLVYARRMANNEVRIIGGQWTGRKLKFPDRPGLR
                     PTLGRVRETLFNWLAPRIGNARCLDLFAGSGALGFEALSRGAAEVTFVEKDRKAAAAI
                     HSNVTALGADAHVKNLPADRFVARDTNVYDVIFFDPPFEDARSLSLLARVLRERLAPG
                     GVLYLEMPRRAALPAIGQLLKRGTAGDCQFVLLTAGE"
     gene            3415..>3532
                     /locus_tag="AC16PP_09655"
     CDS             3415..>3532
                     /locus_tag="AC16PP_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HIR6481934.1"
                     /translation="MNVVVYPGSFNPITNGHTDLISRALSLFDKVILAIGTSA"
BASE COUNT          613 a         1257 c         1065 g          597 t
ORIGIN      
        1 ccagggcacc gagaacaccg aggatcgcgc cgatcccgag caggccgccc aggaacgccg
       61 cgtcgaaacc gaccagatcc agctcctcac cataactgcc aaacaaattg cttaatggtg
      121 cctccacaat cagcaggcac aggctgatca gcatggccgc caccagcccg ccgcccaccc
      181 cgtagaacgc cccgaagtag aggaacgggc gccgcatctg ccgctcggta gcaccaatga
      241 gcttgagcac cttgagttcc tcgaggcgtg cttcgatggc gaggcggacc gatgtggcgg
      301 tcaccagcag ggcacccgtt ccaaacagca gccccagaat gatcccgagc ctgctgacga
      361 ccgaagagat gtccgatacc cgctcgagcc aggttttttc cagcaccacc tcggccaccc
      421 cggcctgctt gcgcacctgt tcgatgagcg ggtccagcgc agcggcgtcc gtctggggaa
      481 ccagggtggc cttgagcgac gcgggcagcg gattgcgttc gagaagatcc aacgcgtcgg
      541 caagctccgt gtagcgccga aactcggcaa gggcctcttc gggattggtc acgctcacgt
      601 ccgtgacccg cgcatcggac ttcagcgcct cgaccgcgga ctgaatctgc gccgtactcg
      661 cgccgagctc gaagtaagcg gtgagcccgg gacgcccctc ccatgccgcc gtcatgccgg
      721 ccaggttccg ctcgatgaga taaaggcctg caggcagcgc gagggcaatg ccgaccagca
      781 gccagacgag caggctggtg cccatccggg cgctgacgaa gtgcacggtc tccctggcga
      841 tacgagcgtg atcacgcccc caggcgccta cgccggaccg caccggcggc caggggtctc
      901 ccgtgcgacg cccactgctc ggctgcggat ccgcggactc agacattgcc gacctcgtca
      961 tccgggtcca cgagccgggc gtgcacgccg tccctcacta ccgcaccctg atcgagctcg
     1021 atgatccgga cacccatgga ctcgatgagg tcgcggtcgt gagatgccac gatcacggtg
     1081 gtgccgacct gattgaacag ctcgaaaaga ttcatgatgt cgcgggacag ctccggatcc
     1141 aggttgccgg tcggctcatc ggccagcagg atcttcgggc gattgaccac cgcccgggcg
     1201 atgcccaccc gctgctgctc gccggtggac agataacgcg ggaacagccg ctccttctcg
     1261 agcagcccga cccgcgacag cgcggcccgg actcgtcggg cgatgtccct ctccttcatg
     1321 ccggtgatcc gtagcggcag cgaaacgttg tcgtagacgc tgcggtggct gagcaggtgg
     1381 tggtcctgga acacggcgcc caggtgctgc cgatagtggg cgagtcggcg cccccgcatc
     1441 cgcgtcacgt tgaccccgtt caccaggatc tgcccgcgcg acggctggtc caggcagagc
     1501 aggagcttga gaaacgtgct cttgccagcg cccgagtgac cggtgagaaa cgccatggag
     1561 ccttccgcga aggcgacgtc cacattgacc agcgcttcct ggccgctgtc aaaacgcttc
     1621 gtcaccccgt gaaattcgat ctgcgccatc tctgatcctg ttggaggaat cctttcagcg
     1681 tgcgggcgcc gcacgggcac gtcatcagct gcgcccggcg ccgttctccg tttccggcag
     1741 caatgccgca acgaaatcac ccggcacgaa cggccgcaga tcgtccgggc cctcacccac
     1801 cccgatatag tagacgggaa tctctctgcc cgtgtccttc aggctgcgca cgatggcgaa
     1861 gatcacaccg gcctttgccg tgccgtccag cttggtcagc accagcccgg tcacgccgac
     1921 cgccgcatcg aattcgcgca cctggctgag cgcgttctgg cccacgcctg catccagcac
     1981 gagcagcacc tcgtgcggag cgccctgctc gagccgcccg acgacccgct gaaccttctt
     2041 cagctcctcc atcaggttgg tcttcgcctg cagacgaccg gccgtgtcgg ccagcacgac
     2101 gtcggtgccg cgcgatcgcg ctgactgaac ggcgtcatag accaccgacg cgctgtccgc
     2161 accctgaccc tgggcgatca ccgggacacc ctcccgttcg ccccagctcg acagctgctc
     2221 gacagcggcc gcgcggaaag tgtccccagc cgcaagcagc accgacagcc ccctcgcctt
     2281 cagctgcctg gccagcttgc cgatcgtcgt ggtcttgccg acaccattga caccgacgaa
     2341 gaagatgaca aacgggcgca cgttctctgg aatctgcagc ggtcgggcga acggcgctag
     2401 gacgttctcc agaagctccg caagtgcctg gtagagcgca cccgtgtcgt tcagctcccg
     2461 gcgcttcacc ctcgccgtca gctgggcaat gatctcccgg gtggtgtcca tacccacgtc
     2521 cgtaagcagc agcgccgttt ccagctcctc cagcgcgacg tcgtcgattt ccttctcgcc
     2581 gaggatcagg ttgcccatcc cggccgcaag ctgggtacgg gtcttgccga ggccggactt
     2641 cagtcgaccc cagaagccgt ttgccgcggc tttcgaactg ttcccgtctt ctccttcgct
     2701 cacctggaat cccacatgca gtttcgccgg ctgcgccttc gagcaggcct cgtgtacgct
     2761 cggcggatgg ccaacaatga agttcgcatt atcggcggtc aatggacggg ccgaaagctc
     2821 aagtttcccg accgccccgg attgcgcccc acgctcggca gggtacgcga gacgctgttc
     2881 aactggctcg cccctcgaat cggcaacgcc cgctgcctgg acctgttcgc cggcagcggc
     2941 gcgctgggat tcgaggcgct ttcccggggc gccgccgagg ttaccttcgt ggaaaaggat
     3001 cgcaaggccg cagcggccat ccactcgaac gtgaccgcgc tgggcgccga cgcccacgtg
     3061 aagaacctgc cggcggacag gttcgtcgcc cgggacacca atgtctacga cgtcatcttc
     3121 ttcgatccgc cgttcgaaga cgcccgctcc ctgtcactcc tcgcgcgcgt cttgcgcgag
     3181 cgcctggctc ccggaggtgt gctgtatctg gagatgccgc gccgcgccgc gctccccgcc
     3241 atcggccagc tgctgaagcg cggcaccgcc ggcgactgtc agttcgtgct gctgacggcc
     3301 ggggaatgac cagttgcctt gccactgctt gcccgcctcg ataccatccc ggcgcctggg
     3361 ggatttggag atgaggatgc acgccatgga tcacgacggg ggaatgaagg gccgatgaac
     3421 gtcgttgtct atcccggatc cttcaacccg atcaccaatg gtcacaccga tctgatttcc
     3481 cgagcgctgt cgctgttcga caaggtcatc ctcgcgatcg gtacttccgc gc
//
LOCUS       DBNHKV010000308         5017 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1106620, whole genome shotgun sequence.
ACCESSION   DBNHKV010000308 DBNHKV010000000
VERSION     DBNHKV010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1106620"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="AC16PP_09660"
     CDS             <1..366
                     /locus_tag="AC16PP_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013767.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_function: GO:0043424 - protein histidine kinase binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR6481935.1"
                     /translation="DLDTAIRGHNGNVRLMLKLMGDFGRYYGDAGAKMRAHVTDNHLE
                     EAERLAHNLHGVAGSFGADRLKQASKTLELALAKGDESNLLGLVQSFEVAMNEVLESA
                     ETLANHPFTEAASRSRNEG"
     gene            complement(329..1180)
                     /gene="ttcA"
                     /locus_tag="AC16PP_09665"
     CDS             complement(329..1180)
                     /gene="ttcA"
                     /locus_tag="AC16PP_09665"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HIR6481936.1"
                     /translation="MRKHGYDMNKLHRKLRRKVGQAIADYGMIEEGDRVMVCLSGGKD
                     SYTLLDILLNLQRSAPVAFEIVAVNLDQKQPGYPADVLPGYLEGLDVSYHILEQDTYS
                     IVKAVTPAGKTTCPVCSRLRRGILYNFAASIGASKIALGHHLDDVVETLFLNMFHGAR
                     LKAMPPKLKSDDGRNVVIRPLYYCRERDIARWAEHREYPIIPCNLCGSQENLQRQVIK
                     SMLARWDAEQPGRVENIARSLSAVVPSHLGDRGLFDFAGLSSDDGVEKLQLLDLTPRC
                     VNAMPPR"
     gene            complement(1294..3786)
                     /locus_tag="AC16PP_09670"
     CDS             complement(1294..3786)
                     /locus_tag="AC16PP_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6481937.1"
                     /translation="MSIKLVLRLAWRDWRGGELGLLLVALMLAVGTVTAVSLFVDRLH
                     QALIDESADFLAADRRISGSRPIPDEFRLAASAKGVEHADTMSFPSMVFVGDDNSQLV
                     SVKAVGAGYPLRGVLKIADEPFAPGVPTSDIPAPGEVWLDARLFPALGIRPGDSIEVG
                     VAPLKVAKVLTSEPDRGGSMYDLGPRLLMRLDDVPATQVVQPGSRIAYRLLLRGAESD
                     LESLKAELPLAPNYRWQGIRESSPSIGSALARAESFLLLGGLLGVLLAGVAVALSAHR
                     YALRHYDHVGILKTLGATPNQILSGYLMLLLAVGSAAVVLGLTGGAALHLGIVALLQS
                     LIPVDLPMPSARPFLLGAATGLICAVAFALPPLLHLKNVSPMRVIRRDLGAAPPSQYL
                     SYGAAVAGSVGLLIWYSGSASLTAWTILGTVVVLLVFGALAMLLLRGGRAVGMQAGSG
                     WRLALAGLQRRRRENVAQILIFGLAIMLLLILVLLRSSLLDEWQGQVPEDAPNHFVLN
                     IVPHEVDELATMLDDFTGSHGGLFPMIRGRIVAANGVDADAWERDHQPVDAPGPRLQS
                     ERNLTWAADLPDDNQVVKGRWWSPDEARALVSLEDEYAQSLGLDVGDRLRFDIAGRFV
                     EVEVASLRRLDWESMRPNFFIIFAPQVLSDFPATYMTSFHLERDEKPFLNRFLARFPT
                     VTVIEVDAIIAQVQSIILRVTQAVELVLALVVASGCLVLVASIQASRDGRMSELALVR
                     TLGGSRRLIAGSLVAEFAALGLFAGTVAVVGAEATVAILQSQVFELPVRLHPWLWLLG
                     PVIGSVLILIVGTLGTRGLISTPPVMVLRGLD"
     gene            complement(3783..4457)
                     /locus_tag="AC16PP_09675"
     CDS             complement(3783..4457)
                     /locus_tag="AC16PP_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6481938.1"
                     /translation="MTNQPVIRTEGVSKSVPTSEGELSILESIDLEINAGETVAIVGA
                     SGSGKTTLLGILAGLDLPTSGRVYLVGEELTALDEEQRARVRGEQVGFVFQTFQLLRS
                     LTALENVMLPGELRGDGDAQPEALRVLDKVGLGARTGHYPTQLSGGEQQRVAIARAFA
                     SRPNVLFADEPTGNLDTATGQRIIDLLFELNAEYATTLVLVTHDDRLASRCERVVEIV
                     AGRITS"
     gene            4477..>5017
                     /locus_tag="AC16PP_09680"
     CDS             4477..>5017
                     /locus_tag="AC16PP_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017641914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HIR6481939.1"
                     /translation="MIALIAGVSPASARSKILVMGDSISAAYGIQRDAGWVALLDRRL
                     SETASPWQVVNASISGETTGGGLARLPKALEAHDPEIVIIELGGNDGLRGYPVSRIRE
                     NLDKLVELAVTGGRRVLLLGMRIPPNYGPRYTRAFEDMYRDVAEAHGVRFVPFILEAV
                     ALNPGLMQDDGIHPTAEAQQ"
BASE COUNT          960 a         1655 c         1627 g          775 t
ORIGIN      
        1 gacctggaca cggccatcag gggacacaac ggcaacgtcc gcctcatgct gaaactcatg
       61 ggggatttcg gcaggtacta cggcgacgcc ggggcaaaga tgcgtgccca cgtgaccgac
      121 aaccatctcg aggaggccga gcggctcgcc cacaacctgc acggcgtggc cggcagcttc
      181 ggggccgacc gcctcaagca ggcttcaaag accctcgagc tcgctctggc caagggcgac
      241 gagagcaacc tgctcgggct ggttcagagt ttcgaggtgg ccatgaacga ggtgctggaa
      301 tcggcagaga ccctggccaa ccaccccttc accgaggcgg catcgcgttc acgcaacgag
      361 gggtgagatc gagcagctga agcttctcga cgccatcgtc cgaggaaagg ccggcgaaat
      421 cgaaaaggcc gcggtcgccg agatgggacg gcaccacggc actgaggctg cgggcaatat
      481 tctccacgcg tccgggctgc tcggcgtccc agcgggccag catcgacttg atcacctgcc
      541 gctgaagatt ctcctgggaa ccgcagaggt tgcaggggat gatgggatac tctcggtgct
      601 cggcccagcg ggcaatgtcg cgctcccggc agtagtagag cggtcgtatc accacgttgc
      661 gtccgtcgtc gcttttcagc ttcggcggca tggccttcag ccgcgcccca tggaacatgt
      721 tgagaaacag cgtttccacc acgtcatcaa ggtggtgtcc cagcgcgatc ttgctcgcgc
      781 cgatgctcgc agcgaagttg tagagaatgc cgcgccgcag ccgcgagcag accgggcagg
      841 tcgtcttgcc cgccggagtg accgccttga cgatggaata cgtgtcctgt tccaggatgt
      901 gataggaaac gtccagccct tccaggtacc cgggcagcac gtccgccgga tacccgggct
      961 gcttctgatc caggttaacg gcgacgatct cgaaggcgac cggcgcgctt ctctggagat
     1021 tgagcagaat gtccagcagc gtgtaggaat ccttgcctcc cgacaggcag accatcaccc
     1081 gatcgccttc ctcgatcatt ccgtaatcgg cgatggcctg gccaactttg cgcctgagct
     1141 ttctgtgcag cttgttcatg tcgtagccgt gcttgcgcat tgcgcgctcc gacctcgcgg
     1201 cggggtccat tgggtctgcc ttcatcaggg ccttgggatg gcgtgggaac ggatttttag
     1261 cagcacggat cggcgctgac aactcacgac gggtcaatcc agaccgcgca gcaccatgac
     1321 ggggggcgta gagatcagcc cgcgcgtgcc cagggtcccg acgatgagga tgagcacgga
     1381 cccgatcacc ggacccagga gccacagcca gggatgcaga cgcaccggca gctcgaaaac
     1441 ctggctttgc aggatggcca ccgtggcttc cgccccgacc acggcgaccg tgccggcaaa
     1501 caggccgagc gcggcgaatt cggcgaccag cgaccccgcg atgaggcggc ggctgccccc
     1561 cagcgtgcgg accagcgcga gctcactcat gcggccgtcg cggctggcct ggatgctggc
     1621 caccagcacc aggcatccgg acgccaccac cagggcgagc acgagctcaa cggcctgggt
     1681 cactctgagg atgatgctct gaacctgagc aatgatggca tcgacttcga tgacggtgac
     1741 cgtgggaaac ctggccagga accggttgag aaacggcttc tcgtcccgct cgagatgaaa
     1801 gctggtcata taggtagcgg ggaaatcgct gagcacctgg ggagcgaaga tgatgaagaa
     1861 attggggcgc atgctctccc aatcgagccg ccgcaggctg gcgacttcca cctccacgaa
     1921 gcgaccggca atgtcgaagc gcagccggtc gccgacgtcc agcccaaggc tctgggcata
     1981 ctcgtcctcg agggaaacca gggcccgggc ctcgtccggc gaccaccagc gtcctttgac
     2041 cacctgattg tcgtccggca ggtcggcggc ccaggtgaga tttcgctccg actgaagtcg
     2101 cgggcccggc gcgtccaccg gttgatggtc ccgctcccac gcgtcggcat ccaccccgtt
     2161 ggcggccacg atgcggccgc ggatcatggg aaacaggccc ccgtggctgc ccgtaaaatc
     2221 gtccagcatg gtcgcgagct cgtccacctc gtgcggaacg atgttgagaa cgaaatggtt
     2281 gggcgcgtct tccggcacct gtccctgcca ctcgtcgagc aacgacgagc gaagcaggac
     2341 caggatcagc agcaacatga tggcgagacc gaaaatcagt atctgggcca cgttctcccg
     2401 acggcgacgc tgcagaccgg ccagcgccag ccgccacccg ctgcccgcct gcatgccgac
     2461 cgcgcgaccg ccgcgcagca gcagcatcgc gagagccccg aataccagca ggacgaccac
     2521 ggtgccgagt atggtccacg ccgtgaggga cgcgctgccg ctgtaccaga tcagcaggcc
     2581 cacgcttccc gccaccgcag ccccgtaact cagatactgg gacggcgggg cagcaccgag
     2641 gtccctgcga atcactcgca tgggagagac gttcttgaga tgcagcaagg gcggcagggc
     2701 aaacgccacc gcgcagatga ggcccgtggc ggcgcccagc aggaacgggc gcgcgctcgg
     2761 catcggcaga tccaccggga tcagagactg cagcaaggca acgatcccca ggtgcagggc
     2821 cgcgcccccg gtcaggccca gcacgacggc ggcgcttcct acggcgagca ggagcatgag
     2881 gtagccggaa aggatctgat tcggcgtcgc gcccagggtt ttcagaatgc ccacatgatc
     2941 gtagtggcga agcgcgtagc gatgcgcgct caatgcgacc gccacgccgg ccagcagaac
     3001 gccgagcaaa ccaccgagca gcagaaagct ctccgcgcga gccagggccg agccgatgga
     3061 gggactgctt tcgcgaatgc cctgccaacg gtagttgggg gcgagcggca gctcggcctt
     3121 gaggctctcg agatcggatt ccgcaccacg cagcagcagg cgataggcaa tgcggctgcc
     3181 gggctgcacg acctgcgtgg cgggcacgtc gtccagccgc atgagcaggc gcggccccag
     3241 gtcgtacatg ctgccgcccc gatccggctc tgaggtcagc acctttgcga ccttgagcgg
     3301 ggcaacgcct acctcgatcg agtcccccgg tcgaattccc agcgcaggga agaggcgcgc
     3361 atccaaccag acctcccccg gcgccggtat gtcagatgtc ggcacgccgg gcgcaaaggg
     3421 ctcatcggcg atcttcagga cgcctcgcag cgggtagcct gctccaacgg ctttgacgct
     3481 gaccagctgg ctgttgtcgt caccgacgaa aaccatggag gggaaggaca tggtgtcagc
     3541 gtgctccacg cccttggccg acgccgcaag ccggaactcg tccgggatcg ggcggctgcc
     3601 gctgatgcgt ctgtccgcgg cgagaaagtc ggcggattca tcgatgagcg cctggtgcag
     3661 tcggtctacg aagaggctca cggccgttac cgtccccacc gccagcatca gggcgacgag
     3721 caggagaccg agctccccgc cacgccagtc gcgccaggcc agccgcagca ccagcttgat
     3781 gctcacgagg taatcctgcc tgccacgatc tccaccaccc gctcacaacg ggaggcgaga
     3841 cgatcatcgt gggtgacgag aaccagcgtg gtggcgtact cggcgttgag ctcgaagagc
     3901 aggtcgatga tgcgctggcc cgtggcagta tccagatttc cggtgggctc atccgcaaac
     3961 agcacgttgg gccgggaagc gaaagccctc gcgatggcaa cccgctgctg ctcaccgccg
     4021 gaaagctgag tcggatagtg ccccgtgcgc gcgccgagcc cgaccttgtc cagcacccgc
     4081 agagcttcgg gctgcgcatc cccatcgccg cgcagctcgc cgggaagcat gacgttctcc
     4141 aacgcggtca ggctgcgcag aagctgaaaa gtctgaaaga cgaacccgac ctgctcgccg
     4201 cgaacccgcg ccctctgctc ctcgtcaagg gccgtcagct cctcaccgac gagatagacc
     4261 cggccgctgg tcggcaggtc gagcccggcg agaataccca gaagcgtggt cttgcccgag
     4321 ccggaggcgc caacgatggc gacggtttct cccgcgttga tttcgaggtc gatgctctcc
     4381 aagatggaga gttccccctc actcgtcggc acgcttttgg acaccccttc ggtacgaatt
     4441 accggctggt tcgtcaaatc ggtattcctg accctgatga ttgcgctgat cgcgggcgtc
     4501 tcgccggcca gcgcgcggtc gaaaattctg gtgatgggcg acagtatcag cgctgcgtac
     4561 ggtattcaac gcgacgccgg ctgggtcgcc ctgctggacc ggcgactcag cgaaaccgca
     4621 agcccgtggc aggtggtcaa cgccagcatc agcggcgaaa ccacgggcgg agggctggct
     4681 cgcctgccca aggccctcga agctcacgat cccgagatcg tcatcatcga gctgggcggc
     4741 aacgatggtc tgcgcggcta cccggtgagc cgcatacggg aaaatctcga caagctcgtg
     4801 gagctggccg tgacgggcgg gcgcagggtg ctcctgctcg gcatgaggat cccgcccaac
     4861 tacgggcccc gctatacgcg cgctttcgag gacatgtatc gcgacgtcgc cgaggcccac
     4921 ggcgtgcgtt tcgtgccttt catcctggag gcggtggcct tgaacccggg cctgatgcag
     4981 gacgacggga tccatccaac ggctgaggcg caacaaa
//
LOCUS       DBNHKV010000309        17711 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1037048, whole genome shotgun sequence.
ACCESSION   DBNHKV010000309 DBNHKV010000000
VERSION     DBNHKV010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 17711)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17711)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17711
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1037048"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="AC16PP_09685"
     CDS             complement(<1..353)
                     /locus_tag="AC16PP_09685"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR6481940.1"
                     /translation="MTIKSDRWIREMAAQGMIEPFEPQQVRLRGDEKVISYGTSSYGY
                     DVRCARNFKVFTNIYSATVDPKAFDERSFVDVTGDVCIVPPNSFALASTVEYFRIPED
                     VLTICLGKSTYARCG"
     gene            complement(389..1414)
                     /gene="apbC"
                     /locus_tag="AC16PP_09690"
     CDS             complement(389..1414)
                     /gene="apbC"
                     /locus_tag="AC16PP_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009876885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HIR6481941.1"
                     /translation="MKIEQFPDPYLNVSWGELGARVLSSGNQVAVTLGYPAAGIRDRL
                     AGQLADFLGVSAVDLDLRFQAPPGRGFSRVKHIIAVASGKGGVGKSTTAVNLALALDR
                     EGARTGLLDADIYGPSQGMMLGVAEGRRPEVRDQQYFEPIKAHGIQAMSMSFLVNEDT
                     PMVWRGPMVSGALQQLLNQSLWDDLDYLVVDMPPGTGDIQLTLSQKVPVSGAVIVTTP
                     QDIALLDAKKGIEMFRKVDIPVLGVVENMSVFRCPHCGGESAVFGTGGGARIAAETGV
                     PVLAELPLAMQIREQADGGLPTVVQDPTGEIAGLYREAARHLAARLWGIEARADADMP
                     EIRITDD"
     gene            1543..3564
                     /gene="metG"
                     /locus_tag="AC16PP_09695"
     CDS             1543..3564
                     /gene="metG"
                     /locus_tag="AC16PP_09695"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR6481942.1"
                     /translation="MTRRILVTSALPNANGAIHIGHLLEHIQTDIWVRFQRMRGHLVI
                     YVCADDTHGTATMLKAEEEGVEPERLIERIRAEHARDFQGFLVAHDNYYSTHSQENRH
                     YAELIYQRLKDGGLIFTREVAQLYDPQRKLFLADRFVKGRCPRCDAEDQYGDNCEACG
                     ATYDATELKDPRSLISGAVPVLERSEHHFFDLPQYTEMLKAWTAGGAVQPEVQNKLAE
                     WLDAGLKPWDISRDAPYFGFLIPGTQDKYFYVWMDAPIGYMASFQNYVSERKDVDFDT
                     YWEPEGDAEVHHFIGKDIVNFHALFWPAVLEGAGFRKPTRLHVHGFVTVEGRKMSKSR
                     GTFVNASTYLEHLNPEFLRYYYATKLNGTIDDMDINLDDFVQRVNSDLVGKVVNIASR
                     CAGFITKQFDGRLAGTLHDAELQGRFVDAAAPIAELFEAGDTSKAVREITALADQANQ
                     YIAHHAPWSMIKEAGREAEVQAVCSQGINLFRILAIYLKPILPRMASEAEAFLEIEPL
                     AWKDVDAPLLDHPIGAFRPLFTRMERKAVDRMIEAGREAGSEPVPDSAPSSEAPAESQ
                     QISIDDFARIDLRVAKISAAEPVAGADKLLRLTLDLGDSNRQVFAGIKSAYEAQSLQG
                     RLCVVVANLAPRKMRFGVSEGMVLAAGPGGKDIFLLSPDSGAEPGMIVR"
     gene            3571..4149
                     /gene="rsxA"
                     /locus_tag="AC16PP_09700"
     CDS             3571..4149
                     /gene="rsxA"
                     /locus_tag="AC16PP_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HIR6481943.1"
                     /translation="MAELALIVVGALLVNNFVLAQFLGLCPFIGVSGNYDTAVAMGFA
                     TTFVLTLAAAVSYMAYHWLLLPLGLEFLRVIVFIVVIAALVQLTEVFLRNTSPVLHNA
                     LGIYLPLITTNCAVLGVALLAINHSLSLLGTVAYAIGAAVGFTMVLVVFAAMRERLAH
                     AEVPAAFRGAPITLISAGILSLAFLGFAGLGN"
     gene            4169..5083
                     /locus_tag="AC16PP_09705"
     CDS             4169..5083
                     /locus_tag="AC16PP_09705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HIR6481944.1"
                     /translation="MLTALVLTAVLTAVVAALGAALVWADRRYPPDTRTLVESIDGLL
                     PQTQCAQCGYPGCRPYAEAVAAGAAINLCPPGGPETHRALARLLGRSEEDPPQEASKL
                     KARIREDGCIGCYLCVEACPVDAIVGAPRYLHTVLEAACTGCELCVPACPVDCIDMTP
                     AREASPISHQPRRRWPDRQSSPCIRCGACQPACPVGLSPQELLWYAGGSSSGRPTGQA
                     AAATRGLDRCIECGLCNPVCPSNIDLVGVFRQARRAQAARHEAERDAADAKARHARRT
                     ERLEQTTAKRAEDREQRIARRSGRQWRA"
     gene            5071..5982
                     /locus_tag="AC16PP_09710"
     CDS             5071..5982
                     /locus_tag="AC16PP_09710"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR6481945.1"
                     /translation="MAGLSSRGIMMAVCLALLPGTATMIYFYGVGVVHNLLLAVPVGL
                     AVEAICLQLQQRSVGRTLADGSTVLTCVLIALALPPGAPFEILSIAVVAAVALAKHLY
                     GGLGSNLFNPALVGYAVVLVSFPVAMASWPIPMDGETGATALVSMKYREGLTVAEAWQ
                     GARGFGAIGGYGWEWINLGFLLGGAVLVWRRLAAWRVPATILATIAVLAAAFWDGGSS
                     TSSGSPLFHWFTGSTMLTVFFFATDPVTHPVSPRGQIVFGIVVGTATFLIRAFGNYPD
                     GAAFAILLGNALTPYLDKRLQEASANA"
     gene            5987..6463
                     /locus_tag="AC16PP_09715"
     CDS             5987..6463
                     /locus_tag="AC16PP_09715"
                     /inference="COORDINATES: protein motif:HMM:NF016121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR6481946.1"
                     /translation="MLIAAACAMLLALTDAWTADRIARNARSHELSQITDLAGAAPPA
                     DTTWNEDVWDFCSGTKLARSSAAGYGGPIRLVVGVSQGRILGIRVTAHQETPGLADFL
                     REPKRGWLAALNGRTGPETAAVDAVAGATITSEAVVRAVRDAMDRAQDASAEGCRA"
     gene            6460..7125
                     /gene="rsxE"
                     /locus_tag="AC16PP_09720"
     CDS             6460..7125
                     /gene="rsxE"
                     /locus_tag="AC16PP_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HIR6481947.1"
                     /translation="MSVAKPFRNAAIASNPAWAQLLGLCPLLAVSTSLVNALALGAAS
                     AFVLLGSNITIAAIRRWIPELARLPSFVLVIATFTTCAMLLMQAYAFELYLRIALFVQ
                     IIVTNCMILGRVEAFASRQPVGRAFLDAAGTACGFALALLAMGAARELIGHGSLMAGM
                     EQLIPGSESWRLNLSESGRGLLLASLPPGAFIIAGLLLGAGNAILRRPNPAPSGSAGE
                     NSA"
     gene            7122..7763
                     /gene="nth"
                     /locus_tag="AC16PP_09725"
     CDS             7122..7763
                     /gene="nth"
                     /locus_tag="AC16PP_09725"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR6481948.1"
                     /translation="MNNDKRRAIFERLRAANPAPTTELDYRNPFELLIAVILSAQATD
                     VSVNKATGPLFEAAGTPTEMKALGLRKLKSYIKTIGLFNTKAANILKTCEMLLTQHGG
                     EVPEDRQALEALPGVGRKTANVVLNTAFGHPTIAVDTHIFRVCNRTRIAPGKNVNQVE
                     QKLLKVVPDEFKRDAHHWLILHGRYTCIARKPRCGSCVIEDLCEFRQKTDLEG"
     gene            complement(7796..8362)
                     /locus_tag="AC16PP_09730"
     CDS             complement(7796..8362)
                     /locus_tag="AC16PP_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481949.1"
                     /translation="MNNYQVLFSGEVSPQANRDIVRAKLACELDIDDRKAAQLFSGRT
                     VVIKSQLSHRQAMELQERLASLGAICRVKDLTPKTRNNVADFRVDRSGKSDATLRDIT
                     AAHTECPRCGHMQLEAAHCTRCGVDIAAAVRQRQKEDLIIEKKIRELRAKQDGLLGRA
                     GAAPGKALQGAASSTVKTRGIGWFRKSS"
     gene            complement(8366..9577)
                     /locus_tag="AC16PP_09735"
     CDS             complement(8366..9577)
                     /locus_tag="AC16PP_09735"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR6481950.1"
                     /translation="MKIVLAYSGGLDTSVICRWLQETYGAEVVTFTADIGQGEEVEPA
                     RAKAQAMGVQEIFIEDLRDEFVRDYVYPMFRANTLYEGEYLLGTSIARPLIAKRLVEI
                     AAETGAEAVAHGATGKGNDQVRFELGAYALNPDIRVIAPWREWDLNSREALMAFCEQH
                     QIPVDYQRGAKSPYSMDANLLHISYEGDNLEDPWQEADADMWRWTVAPEEAPDEAQLV
                     EIDFLQGDPVAVDGEKLGPADLLARLNSLGGAHGIGRSDIVENRFVGMKSRGCYETPG
                     GTLLLKAHRAIESLTLDREVTHLKDELMPRYATLIYNGYWWSPERKVLQALIDESQKP
                     VSGRVRLKLYKGAVSVVGRMSQEESLYDANVVTFEDDGGAYHQADAAGFIRLNALRLR
                     LGARRGRKLSE"
     gene            9746..10333
                     /gene="rnt"
                     /locus_tag="AC16PP_09740"
     CDS             9746..10333
                     /gene="rnt"
                     /locus_tag="AC16PP_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HIR6481951.1"
                     /translation="MADRFRGFLPVVVDLETGGFDPGSHAILEIAAVQLRFDRGRIAP
                     DRKWHQAVEPFPGSRVEAASLKVTGIDLKDPNRQAAPENDALRGLFREVRRAVREAGC
                     TRAILVAHNAAFDQQFLNNALARQQIKRSPFHPFSFIDTASLAAVTYGHTVLSEACAR
                     AHIEFEPARAHNALYDAERTAELFCAIVNAWGRPR"
     gene            complement(10341..10667)
                     /gene="grxD"
                     /locus_tag="AC16PP_09745"
     CDS             complement(10341..10667)
                     /gene="grxD"
                     /locus_tag="AC16PP_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HIR6481952.1"
                     /translation="MSEEVIATIEEQIATHPILLYMKGSPGAPQCGFSAQTVQCLVAC
                     GERFAYVDVLDNPEIRAALPGYANWPTFPQLWVRGELVGGCDIITEMFQAGELEPLIK
                     EAAETE"
     gene            10794..11972
                     /locus_tag="AC16PP_09750"
     CDS             10794..11972
                     /locus_tag="AC16PP_09750"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIR6481953.1"
                     /translation="MPTETGTSEFDVEIDRTNTSSSKWDKYAGRDVLPFWVADMDFAM
                     PSFIRAAIAERLAHPVLGYTRTPDAAVQAFQNWLERCYGWEVPEEWLVWIPGVVTGFN
                     LAARAVAEPGGSVLIPTPVYYPFLSTPRHAGQDSIEVPLVRDGERWAMDFDAMSAACR
                     NGTRLFMLSNPQNPTGRAYTEEELSELAAFCLRHDLYLCSDEIHASIILAPEARHIPI
                     AAIAPEIAERTISLYAATKTYNIPGLGCAVAVIADARLRRDFRRAMGGLVPGIGPLAY
                     AASTAAFADASDWTTRLIDYLRGNHQRVREVVGERMTPVEATYLAWIDLTDLGFADPA
                     ARLEAFGIGLSEGADFGGAGYVRFNFACPRSLLDRGLARFRDALARLDAAQRAGASPD
                     "
     gene            12082..13242
                     /locus_tag="AC16PP_09755"
     CDS             12082..13242
                     /locus_tag="AC16PP_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HIR6481954.1"
                     /translation="MTHLMNNYARLPVAFARGSGAYLEDTSGKQYLDALSGIAVCGLG
                     HAHPRVTRAIAEQAETLLHTSNLYEVPLQERLADRLCEISGMDRVFFGNSGAEANEAA
                     IKIARLYGHKRGEKRPTIVVVDNSFHGRTMATLTATGNRKVQAGFEPLLAGFVRAPFD
                     DLDSVREIAGNNGDVVAVMVEPILGEGGIILPNDDYLPGLREICDQHEWLLILDEVQT
                     GNGRTGRYFAYQHADVLPDVVTTAKGLGNGVPIGACLARGQAAELLVPGTHGSTFGGN
                     PLVCAAAHAVLDALTEDGLIERAASLGERIAAGLKQSLAGNNRVVDIRGRGLMIAVQL
                     NEPCGRLVKAGLEAGILINVTRDDTVRLLPPLTLSDAEADQLVELVSGLVSA"
     gene            13261..14181
                     /gene="argF"
                     /locus_tag="AC16PP_09760"
     CDS             13261..14181
                     /gene="argF"
                     /locus_tag="AC16PP_09760"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR6481955.1"
                     /translation="MAKRDFLTLLDCSGDELSALVARGRELKQNLARRDRPLLGHTAV
                     LILALNSTRTRVGFETGMNQLGGHTVVLNPEDTQLGRGEPIEDMARVLSEMVDVIIIR
                     TLEQTDIERFARAASVPVINAMSAKYHPCQLLADVQTFEELRGPIAGRTVAFVGDGHN
                     MCHSYINAARQWGFNLKIACPEGYSPDPSVLGSTNRASLVESAREAVEGADLVVTDVW
                     SSMGQEEEAARRREQFAPFQVNETLLDHAPDHTVFMHCLPAHRGEEISDKLLDDPRSA
                     VWQEAGNRLHSQKALLEFLILGEFPGSSGG"
     gene            complement(14208..>17711)
                     /locus_tag="AC16PP_09765"
     CDS             complement(14208..>17711)
                     /locus_tag="AC16PP_09765"
                     /inference="COORDINATES: protein motif:HMM:NF019410.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HIR6481956.1"
                     /translation="IRRKMRRITQPTLDALCQKCFELIEEDLREKTLASLGDGLGEIY
                     DSGRELIEKYRNQIRNDFIGGVLRQISNVAQLEDIAPLRSRREEAIRREHEQAGSDKF
                     DGWLDVAEVVAKAESRYRDDLRDLRARLGLVEPPWRHKEVVPVGPAAITWAFDGALKD
                     LELTPRIRTHVFEGFQAALLPALNDFYHGLGELLSECEIFPTEEALEAISALQEPARD
                     DDLTLHEYVPGSARHGEASGDEPDAEEEAAVGVKLATHDEPEAEQVGPAPREVYATAR
                     SIVKLERVARQQLEGEGGEPRQAAGGGAANPFGSEEILRVLGALGPGGQGDRATVCQR
                     LVQELGAADGDRSLGESDLDRFRVLESLIDSICEDRFLSQGSLNWVRRLEVTLYKLAV
                     HNPGFPGGDPDRPHPAEIMLNQLARLSGIADSREAKARELSGRVDALVERVADGYSGD
                     GGIFEEAVAELNPLVDEQIRNYRGNMERIVRASEGQQRLTRARKAVVNALAPKMAGKE
                     VPEPILKLLNPGWRNLMVHTCLRMGAGSAQWEERVAVIERLWAEFVAEPEQGGRDASD
                     LKQLLRQVADGLISISFDPGLRKPLLLDIAKVLRAKADGKPAPVKTVSVAADGVADAL
                     GIARLLPESDAELEAAQQEMQRSWAKSLSRARTLQAGDWIATDDAQGRPLILVVAFVG
                     DESSGFVLANRNGRKERELTLTEMADNLHEARMTLLSDFDMPLVERAAERMLETVHGR
                     LADQSGRDALTQLVNRREFERRLDKALKRSRADGVDHALMLIDLEGVEAVRSDTGDAG
                     ADELLKGAADVLTRALIGRPADIGRVDADRFGILLERTSGPAAMEIAEQILKALGDDP
                     LRVSSGQQALSASIGLVSMDTQSESANAVMHYAERACRAARLSGRNQIKEFEIGEIAQ
                     LRRHGEAAWAFRLDEAMAGEGLIVSFRQIAPMSGARNGVDTHYEVQLTMRDEEGGIMA
                     PEKFQDAAESHERMPALDRWTVAWCLDWMAAHKDSLDHFGGFAIRLSDRSLNEPTFSD
                     FVLERFSACQVPTSKICFEITESVAVANIENAVEFMNRMKIIGCRFALEDFGSGRASY
                     AYLRRLPVDYVKIDDVFVREIAANPGDYAVVRSINEIGHYMGKKTIAEFVEDEEVRAR
                     LAEIGVDYLQGMDAQSAIPLR"
BASE COUNT         2951 a         6089 c         5574 g         3097 t
ORIGIN      
        1 atgccgcagc gggcgtaggt cgacttgccc agacagatgg tgagcacatc ctcgggaatc
       61 cggaagtact ctaccgtgct ggccagcgcg aacgagttcg ggggaacgat gcagacgtcc
      121 ccggtcacgt ccacgaagct tcgctcgtcg aacgccttgg ggtcgacggt ggcggagtag
      181 atgttcgtga agaccttgaa gttccgcgcg cagcggacat cgtagccata gctggacgtc
      241 ccgtaggaaa tgaccttttc atcgcccctg aggcgcacct gctgcggctc gaagggttcg
      301 atcatgccct gcgccgccat ctcgcgaatc cagcgatccg acttgatcgt caccgtgcca
      361 ccccgatctg ctgtggttgg gccatggtct agtcgtccgt gatgcggatc tcgggcatgt
      421 cggcgtccgc acgtgcctca atgccccaca gccgcgccgc gagatgccgc gcggcctccc
      481 ggtacagacc ggcaatctcg cccgtcggat cctgcacaac ggtcggcagg ccaccgtccg
      541 cctgctcccg gatctgcatg gccagcggca gctcggcaag cacgggcaca ccggtctcgg
      601 cagcaatccg cgccccgcct ccggtaccga acacggcgct ctccccgccg cagtgcgggc
      661 agcggaagac gctcatgttt tcgaccacgc cgagcacggg aatgtcgacc ttgcggaaca
      721 tctcgatgcc tttcttggca tccagcaggg cgatgtcctg aggcgtggtg acgatcacgg
      781 cgcccgacac gggcaccttc tgggacagcg tcagctggat gtcgccggtt cccggcggca
      841 tatccaccac gaggtagtcc agatcgtccc acagcgactg gttgagcagc tgctgcaacg
      901 cgccggaaac catgggcccg cgccagacca tgggcgtgtc ctcgttcacc aggaagctca
      961 tggacatggc ctggattcca tgggccttga tgggctcgaa gtactgctgg tccctgacct
     1021 cgggccgccg accctcggcg acgccgagca tcatcccctg gctggggccg taaatgtcgg
     1081 cgtcgagcag acccgttcgc gcgccttccc gatccagcgc cagcgccaga ttcacggcgg
     1141 tggtggactt gcctacgccg cccttgcccg aggcaacggc aatgatgtgc ttgacccggc
     1201 tgaatcctct ccccggaggt gcctgaaacc gcagatcgag atcgacggca ctcaccccga
     1261 gaaaatcggc cagctgcccg gcaagccggt cgcggatacc ggccgcgggg tagcccaggg
     1321 tgacagcaac ctggttgccc gagctgagca ccctggcgcc cagctcgccc cacgaaacgt
     1381 tgagatacgg atccggaaat tgctcgattt tcattaatgc accgcggcag cgcccttgcg
     1441 gggccctgcc tgtggtaaga cgccgaaggc gcgcattata ggtgagttac cgttatagtc
     1501 ccgctttttc ggacaccccg aagcgctcac gcgcagccaa ccatgacacg tcgcatcctg
     1561 gtcaccagcg ccttgcccaa cgccaacggc gcgattcaca tcggtcatct gctggagcac
     1621 atccagaccg acatctgggt gcgttttcag cgtatgcggg ggcacctggt gatctacgtc
     1681 tgcgcggacg atacccacgg cacggccacc atgctcaagg ccgaggaaga gggagtcgag
     1741 cccgaacggc tcatcgagcg gatccgcgcc gagcatgcca gggacttcca gggctttctc
     1801 gtggcccacg acaactacta ctccacccat tcccaggaaa accgccatta cgccgagctc
     1861 atctaccagc gcctgaagga cggcggcctg atcttcaccc gggaggtggc ccagctctac
     1921 gacccccagc ggaagctgtt tctcgccgac cgtttcgtca agggccgctg cccgcgctgc
     1981 gacgccgagg atcagtacgg cgacaactgc gaagcgtgcg gcgcaaccta cgacgccacc
     2041 gagctgaagg atccgcgctc gttgatctcc ggggcggtcc ccgtgctgga gcgctcagag
     2101 caccacttct tcgatcttcc ccagtacacg gaaatgctga aagcgtggac ggccggcggc
     2161 gcggtgcagc ccgaggtgca gaacaagctg gccgagtggc tcgacgcggg cttgaagccc
     2221 tgggacattt cccgggacgc gccgtacttc ggctttctga ttccagggac tcaggacaag
     2281 tacttctacg tgtggatgga cgcgcccatc ggctacatgg cgagcttcca gaattacgtc
     2341 tccgagcgca aagacgtcga tttcgacacg tactgggagc ccgagggcga cgccgaggtg
     2401 catcatttca tcggcaagga catcgtcaac tttcacgcgc tgttctggcc ggcggtgctg
     2461 gagggcgccg ggttccgcaa gcccacccgt ctgcacgttc acggctttgt caccgtcgaa
     2521 gggcgcaaga tgtcgaaatc gcgaggcacg ttcgtcaacg cgtccaccta cctcgagcac
     2581 ctcaaccccg agttcctgcg ctactactac gcgaccaagc tgaacggaac catcgacgac
     2641 atggacatca atctggacga cttcgtccag cgcgtaaact ccgatctggt cggtaaggtc
     2701 gtcaacatcg ccagccgctg tgccgggttc atcaccaagc agttcgacgg gcgcctggcg
     2761 ggcacgctgc acgacgccga gctgcagggg cggttcgtcg acgccgccgc gcccatcgcc
     2821 gagctcttcg aagccggcga caccagcaag gccgtgaggg aaatcacggc gctggccgac
     2881 caggcgaacc agtacatcgc ccaccacgcg ccctggtcca tgatcaagga agcgggccga
     2941 gaagccgagg tccaggcggt gtgcagccag ggcatcaacc tgttccgcat cctggcgatc
     3001 tacctcaagc cgatcctgcc gcggatggcg agcgaagccg aagccttcct cgagatcgaa
     3061 ccgctcgcct ggaaggatgt ggatgcgcct ctgctcgatc acccgatcgg cgcgttcagg
     3121 cctctgttca cgagaatgga gaggaaggcc gtggaccgga tgatcgaggc cggccgggag
     3181 gcgggctccg agcccgtccc ggactccgcc ccatccagcg aggcgcccgc cgaatcacag
     3241 cagatcagca tcgacgactt tgccaggatc gacctgagag tggcgaagat cagcgccgcg
     3301 gagcccgtgg caggcgccga caagcttctg cggctgactc tggatctcgg cgattccaac
     3361 cgccaggtct tcgccggtat caagtctgcc tacgaagcac agagtctgca gggcaggctg
     3421 tgcgtcgtgg ttgccaacct cgctccgcgc aagatgcgct tcggggtatc cgaggggatg
     3481 gtgctcgccg ccggcccggg cggaaaggac atcttcctgc tgagcccgga ctcgggcgcc
     3541 gagccgggca tgatcgtgcg ctgagccgcc atggctgagc tcgctctcat tgtcgtcggc
     3601 gctctgctcg tcaacaattt cgtgctggcc cagttcctcg gcctgtgccc gttcatcggc
     3661 gtgtccggca actacgacac ggcggtggcc atggggttcg ccaccacctt cgtgctgacc
     3721 ctggcggccg ccgtgagcta catggcctat cactggctgc tgctgccttt ggggctggag
     3781 tttctgcggg tcatcgtgtt catcgtggtc atcgctgccc tcgtccagct gacggaggtt
     3841 tttctgcgca atacgagccc cgttctgcac aacgccctgg gcatctacct gccgctgatc
     3901 accacgaact gcgcggttct gggcgtcgcc ctgctggcca tcaaccacag cctctccctg
     3961 ctgggaaccg tggcgtacgc catcggggcg gcggtgggtt tcacgatggt tctggtggtc
     4021 ttcgcggcca tgcgggaacg cctcgcccac gccgaagtgc cggcggcatt tcgcggtgcg
     4081 cccattaccc tgatcagcgc gggaattctc tctcttgcgt tcctcgggtt cgcggggctc
     4141 ggcaactgat gggcagcgca ggtcccccat gctgaccgct ttggtactga ctgccgtgct
     4201 gaccgccgtg gtggccgccc tgggggccgc gctcgtctgg gcggaccggc gctacccgcc
     4261 ggatacgcgg acgctggtgg agtcgatcga cggtctgctc ccccagaccc agtgcgcgca
     4321 gtgcggatac cccggctgcc gaccctacgc cgaggccgtc gccgcgggcg cggccatcaa
     4381 cctgtgccct ccaggcggtc ccgagacaca ccgggccctc gcccggttgc tcggccgcag
     4441 cgaagaagac ccaccgcagg aggcttccaa gctcaaagcc cgcatccgag aggacggctg
     4501 catcggctgt tacctgtgcg tggaggcatg ccccgtggac gccatcgtgg gcgcgccgcg
     4561 ctatcttcac acggtcctcg aggcggcgtg cacgggctgc gagctttgcg tcccggcgtg
     4621 cccggtggac tgcatcgaca tgacgcccgc ccgggaggct tcccccatca gccatcagcc
     4681 gcgccggcga tggccggacc ggcagagctc accatgcatc aggtgcggag cctgccagcc
     4741 cgcctgcccg gtggggctga gcccccagga gctgctctgg tacgccgggg gctcctccag
     4801 cgggaggcct accggtcagg cggccgccgc gacccgcggc ctggatcgct gcatcgagtg
     4861 cgggctttgc aacccggtct gtcccagcaa catcgacctc gtgggcgtct ttcgccaagc
     4921 gcgacgagcg caggccgcaa ggcacgaagc cgaacgggac gccgccgatg ccaaggcgcg
     4981 gcatgcaaga cgcaccgaac gcctcgagca gacgaccgcc aagcgcgctg aggatcgcga
     5041 gcagcgcatt gcccgacgga gcggcaggca atggcgggcc tgagctcccg cgggatcatg
     5101 atggccgtct gcctggcgct tttgccgggc accgcgacga tgatctattt ctacggggtc
     5161 ggcgtggtgc acaacctgct gctcgccgtg cccgtcgggc tggccgtcga agccatctgc
     5221 ctgcagttgc agcagcgttc cgtcggccgg acgctggccg atggcagcac ggtactcacg
     5281 tgcgtgctca tcgcgctcgc cctgccgccc ggcgcgccct ttgaaattct ctccatcgcc
     5341 gtcgttgcgg ccgtagccct ggcaaaacac ctttacggcg ggctggggag caacctgttc
     5401 aaccccgcgc tcgtggggta cgccgtcgtc ctggtctcgt ttcccgtggc gatggcgagc
     5461 tggcccatcc cgatggacgg cgaaaccggc gccacggcgc tggtgtcgat gaagtaccgg
     5521 gaggggctca ccgtcgcgga agcgtggcag ggcgcacgcg gcttcggcgc catcggcggc
     5581 tacggctggg agtggatcaa cctgggcttc ctcctaggcg gggctgtgct cgtgtggcgg
     5641 cggctggcgg catggcgcgt gccggcgacc attctggcca ccatcgccgt gctggcggcc
     5701 gcgttctggg acggcggcag ctcgaccagc agcgggtccc cgctcttcca ctggttcacc
     5761 ggctccacga tgctgaccgt cttcttcttc gcaacggacc ccgttacgca cccggtttcg
     5821 ccgcgcggtc agatcgtctt cggcatcgtg gtgggcaccg cgaccttctt gattcgggcc
     5881 ttcggtaact atccggacgg cgcggcattc gccatcctcc tcggcaacgc cctcacgccc
     5941 tatctcgaca agcgtctgca ggaggcgtcg gcaaatgcat aagctcgtgc tcatcgcggc
     6001 cgcctgcgcg atgctgctcg ccctcaccga tgcttggacg gcggatcgca tcgcccggaa
     6061 cgcccgcagc cacgagctga gccaaatcac cgacctggcg ggagcagcgc cgcctgccga
     6121 tacgacctgg aatgaggacg tctgggattt ctgcagcggc acgaaactcg cccgcagctc
     6181 ggcggcgggt tacggcggcc cgatccggtt ggtcgtcggc gtgtcgcaag ggcgcatcct
     6241 gggcatcaga gtcaccgcgc accaggaaac gcccggcctt gccgacttcc tgcgcgagcc
     6301 caagcgcggt tggcttgccg cattgaatgg ccggaccgga ccggaaaccg cggccgtcga
     6361 tgcggtagcg ggggccacga tcaccagcga agcggtcgtc cgcgcggttc gcgacgccat
     6421 ggaccgggcg caggacgcca gcgccgaggg atgccgcgca tgagcgtcgc caagccgttc
     6481 cgcaacgccg ccatcgccag caacccggcc tgggcgcagc tgctgggcct ctgccccctg
     6541 ctcgctgtca gcaccagcct ggtcaacgcc ctggcgctgg gcgcggcaag cgcgttcgtg
     6601 cttctcggca gcaacatcac catcgccgcc atccgccgat ggataccgga gctcgcccgt
     6661 cttccgagtt tcgtgctcgt catcgccacg ttcacgacgt gcgcgatgct cctgatgcag
     6721 gcctacgcct tcgagctgta tctgcgaatc gcgctgttcg tccagataat cgtcaccaac
     6781 tgcatgatcc tcggccgggt cgaggcgttc gcgagcaggc agccggtggg gcgcgctttc
     6841 ttggacgcgg cgggcacggc ttgcgggttc gcgctggccc tgctcgccat gggcgccgcc
     6901 cgagagctca tcggccacgg ctccctgatg gcgggaatgg agcagctgat tcccggctcg
     6961 gagtcatggc ggttgaacct gtcggagagc ggacgcggtc tgctcctggc gagcctgccg
     7021 cccggcgctt tcatcatcgc cggcctgctc ctcggtgcgg gcaacgcgat tctgcgccga
     7081 ccgaacccag ccccatccgg ctccgcagga gaaaactcag cgtgaacaac gacaaacgcc
     7141 gcgccatctt cgagcgccta cgggcggcca acccggcgcc gacgacagag ctcgactacc
     7201 gcaacccctt cgagctgctg atcgccgtca tcctctccgc ccaggccacc gacgtcagcg
     7261 tcaacaaagc cacgggcccg ctgttcgagg cggccggaac gccgaccgag atgaaggcgc
     7321 tcgggctgcg aaagctcaag agctacatca agaccatcgg cttgttcaat accaaggcgg
     7381 ccaacatctt gaagacctgc gagatgctgc tcacgcagca cggcggcgag gtcccggagg
     7441 atcgccaagc cctggaagcg ctccccgggg tgggacgcaa aaccgccaac gtggttctca
     7501 acacggcgtt cgggcacccc accatcgccg tggacaccca catcttcagg gtctgcaacc
     7561 gcacccggat cgcgccgggc aagaacgtca atcaggtgga gcagaagctg ctcaaggtcg
     7621 tgccggacga gttcaagcgc gacgcgcacc actggctgat actgcacggc cgctatacct
     7681 gcatcgcgcg caagccgcgc tgcggatcat gcgtgatcga ggatctctgc gagtttcgtc
     7741 aaaagaccga tctcgagggc tgaggtatcc gcggcgcggc cgcgcaggcg ggcactcagg
     7801 acgactttct gaaccagccg atgccgcgag ttttcaccgt cgacgaagcc gcgccctgca
     7861 gcgcctttcc gggcgcggcg cccgctcgcc cgagcagacc gtcctgcttg gcgcgcagct
     7921 cgcgtatctt cttctcgatg atcaggtcct ctttctgccg ctgccggacg gctgccgcga
     7981 tgtcgacacc gcagcgcgtg cagtgagcgg cttccagctg catgtgcccg cagcgcgggc
     8041 actccgtgtg agcagcggtg atgtcgcgca gggtcgcgtc ggatttaccg gacctgtcca
     8101 cccggaagtc cgccacgttg ttgcgggtct tcggcgtgag gtctttgacc cggcatatgg
     8161 cgcccaggct ggctagacgc tcctgcagct ccatggcctg ccgatggctg agttgactct
     8221 tgatgaccac cgtccggccg ctgaaaagct gcgcggcttt gcggtcgtcg atgtcgagtt
     8281 cgcaggccag ctttgcccgc acgatgtccc tattcgcctg cggggaaacc tcaccgctga
     8341 acagcacttg atagttgttc atcgattact ccgaaagctt tcgtcctctc cttgcgccca
     8401 agcgcaggcg gagggcgtta aggcggatga aacccgccgc gtcggcctga tggtacgccc
     8461 ccccgtcgtc ctcgaaagtg acgacgttcg catcgtacag gctctcctcc tgcgacatcc
     8521 gtcccacgac ggataccgca cccttgtaca acttgagcct aacccgaccc gagacgggtt
     8581 tctgtgattc gtctatcagc gcttggagga cttttctctc aggagaccac cagtagccgt
     8641 tgtagatgag cgtcgcgtac cggggcatga gctcgtcctt gagatgggtg acttcccggt
     8701 ccagcgtgag ggactcgatg gcgcggtggg ccttgagcag cagagttccc cccggtgtct
     8761 cgtagcagcc tcgggacttc atcccgacga agcggttctc cacgatgtcg ctgcgaccga
     8821 taccgtgggc gccccccagg gaattgagcc tggcgagcag atcggccggt ccgagcttct
     8881 cgccgtcgac ggccacggga tcgccctgca ggaaatcgat ctcgacgagc tgcgcctcgt
     8941 ccggcgcctc ctcgggcgcc accgtccagc gccacatgtc ggcatccgcc tcctgccagg
     9001 gatcttctag gttgtcgcct tcgtaggaga tgtggagcag gttggcatcc atggagtaag
     9061 gagacttggc gccgcgctga taatccacgg ggatctggtg ctgctcgcag aacgccatca
     9121 acgcctcccg ggaattgaga tcccactcgc gccacggcgc gatcacccga atgtccggat
     9181 tcagcgcgta tgctccgagc tcgaagcgga cctggtcgtt gcccttgccg gttgctccgt
     9241 gagcgacggc ctccgcgccg gtttcagcgg cgatctcgac gagacgcttg gcaatcagag
     9301 gccgcgcaat gctcgtcccg agcaggtact cgccttcgta gagagtattt gcgcggaaca
     9361 tcgggtagac gtagtcgcgc acgaactcgt cgcgcagatc ctcgatgaag atctcctgca
     9421 cgcccatggc ctgggccttc gccctggcag gctccacctc ctcaccctgg ccgatgtccg
     9481 cggtgaacgt caccacctcg gcaccatagg tttcctgcaa ccagcggcag atcacggagg
     9541 tatccaggcc acctgagtat gccagcacaa tcttcatcgt cacgtttccc ccgagtgcgg
     9601 tcgccgagaa aaaagggtcg caatcttaag taacttgagc agggcccgcc agccgccgtc
     9661 ccgcgggaaa cccttccggc cgggtgcctt cccgcttccg gcgcgcaaag tcgcgtcaaa
     9721 gccgtgttaa tgtgcccgca tggagatggc ggatcgtttt cggggttttc tgccggtggt
     9781 ggtcgatctg gagacgggag gcttcgatcc cggctcccac gccattctgg agatcgcagc
     9841 cgtgcagctg cgtttcgatc gcgggcggat cgcgccggac cggaagtggc atcaggccgt
     9901 ggagcccttc ccggggagcc gggtggaggc cgcttcgttg aaggtgaccg gcatcgacct
     9961 gaaggatccc aaccggcagg cggcaccgga gaacgatgcg ttgaggggtc ttttccggga
    10021 agtgcgccgg gccgtgcgag aagccggctg cacgcgtgcc attctggtcg cgcacaacgc
    10081 cgcgttcgat cagcagtttc tcaacaatgc ccttgcgcgc cagcagatca agcgcagccc
    10141 tttccatccg ttcagcttca tcgacaccgc gtctctggcc gcggtgactt acggtcacac
    10201 ggtgctcagc gaagcctgcg cgcgtgcgca catcgagttc gagccggccc gggcacacaa
    10261 cgcgctctac gacgccgagc gaacggccga gcttttctgc gccatcgtga acgcctgggg
    10321 gcgtccgcgt tgaacgatcg tcactccgtc tcggcggctt ctttgatcag aggctcgagc
    10381 tcacccgcct ggaacatctc ggtgatgatg tcgcagcctc cgacgagctc gccgcgcacc
    10441 cagagctggg gaaaggtcgg ccagttcgcg tagcccggca gcgcggctct gatttccggg
    10501 ttgtcgagga cgtccacgta ggcaaatcgc tctccgcagg cgaccaggca ctgaaccgtc
    10561 tgggcagaaa atccgcactg cggtgcgccc gggctgccct tcatgtaaag cagaatcgga
    10621 tgtgtcgcga tctgctcctc tatggtggcg atcacttctt cgctcattgc tcgtcaaccc
    10681 tcgacgcgaa ttgcgcttgg catggaatgc cgacattcta ccctccgatc aggccgcgca
    10741 acagggagcc ggagcgcagt ccatcaaggc gcggatcggt gcacggaggg aacatgccga
    10801 ccgaaacggg aacgagcgag ttcgatgtcg agatcgatcg caccaacacg tcgagctcca
    10861 aatgggacaa gtacgcaggc cgcgacgtgc tccctttctg ggtcgcggac atggatttcg
    10921 ccatgccgtc attcatccgc gcggccatcg cggagcgcct cgcgcatccc gtgctgggat
    10981 acacgcgcac tcctgacgct gcggtgcagg cgttccagaa ctggctcgag cgctgctatg
    11041 gctgggaagt gcccgaggag tggctggtct ggatacccgg ggtggtgaca ggattcaacc
    11101 tggccgcgcg cgcggtggcc gagcccgggg gctccgttct cattcccaca cccgtctact
    11161 acccgtttct gtcgaccccc cgccatgcag gccaggactc catcgaggtc ccgctcgtga
    11221 gagacggcga gcgatgggcc atggacttcg acgccatgag cgccgcttgc cgcaacggga
    11281 cacggctgtt catgctgagc aacccgcaga acccgacggg ccgcgcgtat accgaggagg
    11341 agctgtccga gctcgcggcg ttctgcctgc gccacgacct ctacctatgc tcggacgaga
    11401 ttcacgcaag catcattctg gcgcccgagg ctcgtcacat tcccattgcc gccatcgctc
    11461 cggaaatcgc cgagcgcacc atcagccttt acgccgccac caagacctac aacattcccg
    11521 gcctcggctg cgcggtggcg gtgatcgccg atgcgcgcct gcgacgcgac ttccgtcggg
    11581 ccatgggggg gctcgtcccg ggtataggac cgctggccta cgcggcttca accgctgcct
    11641 ttgcagacgc cagcgattgg acgacgcgcc tgatcgacta cctgcgcggc aatcaccagc
    11701 gcgtgcgcga ggtggtcggc gagcgaatga cgcccgtcga ggccacctat ctcgcctgga
    11761 tcgatctcac cgatctcggc tttgccgatc ccgccgctcg cctggaagca ttcggcattg
    11821 gtctttccga aggggcggac ttcggcgggg ccgggtacgt gcgtttcaac ttcgcctgcc
    11881 ctcgatccct gctcgaccgc ggcctggcac gctttcgaga tgcgctcgcc cggttggacg
    11941 cagcgcagag ggcgggtgcg tcgcccgatt gaactgacca ctcccctgca gtacgattca
    12001 gcgtttttcc gggcagcaac agctgcctgg aatggttttg gggaggcgaa aatcttccct
    12061 acgacgatcc gcaggcagca catgacgcac ctcatgaaca actatgcccg cctacccgtg
    12121 gcgttcgccc ggggtagcgg cgcgtatctc gaggacacgt ccggaaagca gtatctggac
    12181 gctctcagcg gcatcgccgt ctgcggcctg gggcacgccc atccccgggt cacgcgcgcc
    12241 atcgccgagc aggcggaaac gcttctccac acttccaacc tctacgaggt tcccctgcag
    12301 gagcggctgg ccgatcggct ctgcgagatc tcaggcatgg accgggtctt cttcggcaat
    12361 tccggcgccg aggcaaacga ggcggccatc aagatcgccc ggctctacgg ccacaagcgg
    12421 ggagaaaagc gacccaccat cgtggtcgtg gacaacagct ttcacggccg caccatggca
    12481 acgctgacgg cgacgggaaa ccgcaaggtg caggcgggct tcgagcccct gctggccgga
    12541 ttcgtccgcg ctcctttcga cgacctggac tcggttcgcg agatcgcggg caacaacggc
    12601 gacgtggtcg ccgtgatggt ggagcccatt ctcggcgagg ggggcatcat cctgccgaac
    12661 gacgactatc ttcccgggct gcgagagatc tgcgaccagc atgagtggct gctcatcctg
    12721 gacgaggtcc agacgggcaa cggccgcacc ggccgctact ttgcctacca gcacgccgac
    12781 gtgctcccgg acgtggtgac gacggccaag ggtctcggca acggcgtgcc catcggtgcc
    12841 tgcctggccc gtggtcaggc ggcggaactg ctggtcccgg gcacccacgg ctccacgttc
    12901 ggcggcaacc cgctggtctg cgccgcggcc cacgcggtgc tggacgcgct gacggaagac
    12961 gggctgatcg agcgcgccgc cagcctcggc gaaagaatcg cagccggcct gaagcaatcc
    13021 ctggccggca acaaccgggt cgtcgacatc cggggtcgcg gcctgatgat cgcggtgcag
    13081 ctcaacgagc catgcggcag gctggtgaag gcgggcctgg aggccgggat tctgatcaac
    13141 gtgacccgag acgacaccgt caggctgctt ccgccgctga ctttgagcga cgccgaggcc
    13201 gaccagctgg tagagctggt gagcggtctc gtctccgcct gacccgcgga acctgccacc
    13261 atggccaagc gcgacttcct cacccttctc gactgttccg gcgacgagct gagcgcgctc
    13321 gtggcgcgag gccgggaact caagcaaaac ctcgcccgac gtgaccgacc gttactcggg
    13381 cacacggcgg tgctcatcct cgccctgaac tcgacccgca cccgggtggg gttcgagacc
    13441 ggcatgaatc agctcggcgg ccacaccgtc gttctcaacc cagaggacac ccagctcggc
    13501 cgcggcgagc ctatcgagga catggctcgg gtgctttccg agatggtgga cgtgatcatc
    13561 attcgaaccc tcgagcagac ggacatcgaa cgcttcgccc gcgcggccag cgtcccggtg
    13621 atcaacgcca tgagcgcgaa gtatcatccc tgtcagctgc tcgccgatgt tcagaccttc
    13681 gaggagctgc gcggtcccat cgcggggcgc acggtggcgt tcgtgggcga tggccacaac
    13741 atgtgccact cctacatcaa cgcggcccgg caatggggct tcaacctgaa gatagcctgt
    13801 cccgaaggct actcacccga cccctcggtg ctcggctcca caaaccgggc aagcctggtt
    13861 gaatccgccc gggaggccgt ggagggagcc gacctggtgg tcaccgacgt ctggtcttca
    13921 atgggccagg aggaggaagc cgcccggcgc cgcgagcagt tcgcgccgtt ccaggtcaac
    13981 gagacgctgc tggaccatgc tccggaccac accgtcttca tgcactgcct gcccgcccat
    14041 cggggcgaag agatcagcga caagctcttg gacgatcccc gctccgcggt ctggcaggaa
    14101 gcaggcaaca gattgcacag ccagaaggcg ctgctcgagt ttctgattct gggggaattt
    14161 ccaggctcgt ccggcggttg atttcaagcc ggaggacggc tgggcgatta ccgcagcggg
    14221 atcgcgctct gggcatccat gccctgcagg tagtccaccc cgatctcggc cagccgggcg
    14281 cggacctcct cgtcctcgac gaactccgcg atggtcttct tgcccatgta gtgaccgatc
    14341 tcgttgatcg agcgaaccac ggcgtagtcg ccgggattgg ccgcgatctc ccggacgaac
    14401 acgtcgtcga tcttcacgta gtctacgggc aggcggcgca ggtaggcata ggacgcgcgg
    14461 cccgacccga agtcctccag ggcgaaacgg cagccgatga tcttcatgcg gttcatgaac
    14521 tccaccgcgt tctcgatgtt ggcaaccgcc accgactcgg tgatctcgaa gcagatcttg
    14581 ctggtcggga cctggcaggc ggagaaccgc tccagaacga agtccgagaa cgtcggctcg
    14641 ttcagcgagc gatccgacag ccggatcgcg aaaccgccga aatgatccag gctgtccttg
    14701 tgagccgcca tccagtcgag acaccaggcc acagtccagc ggtccagggc gggcatgcgt
    14761 tcgtggcttt ccgccgcatc ctggaatttc tcgggggcca tgatcccacc ttcctcgtcg
    14821 cgcatggtca gctgcacttc gtagtgcgta tcgacgccgt tgcgcgcgcc gctcatcggg
    14881 gcgatctggc ggaaactcac gatcaggccc tcaccggcca tggcttcatc cagcctgaag
    14941 gcccacgcgg cttccccgtg gcgccgcagc tgcgcaatct ccccgatctc gaactccttg
    15001 atctgattgc gacccgacag cctggccgcc cggcatgccc tttcggcata gtgcatgacc
    15061 gcatttgccg actcggactg cgtatccata ctcaccagcc cgatgctggc ggaaagcgcc
    15121 tgctggccgc tgctcacgcg caagggatcg tcgccgagcg ccttgaggat ctgctcggca
    15181 atttccatcg cggccggccc gctcgtgcgt tcgagaagaa tgccgaagcg gtccgcgtcg
    15241 acgcggccga tgtccgccgg ccgaccaatg agcgccctgg tcagaacgtc cgcggcgccc
    15301 tttagcagct cgtcggcgcc cgcatctccc gtatccgacc gcaccgcctc gacgccttcc
    15361 aagtcgatga gcatcagggc gtgatccacg ccatccgccc tcgacctctt cagagccttg
    15421 tcgagccgcc gctcgaactc ccgccggttg acgagctgcg tcagcgcatc ccgaccggac
    15481 tggtcggcaa gcctgccgtg cacggtctcc agcatccgtt ccgccgcgcg ctccacgagc
    15541 ggcatgtcga agtcgctcag cagggtcatc cgagcttcgt gcaggttgtc cgccatctcg
    15601 gtcagcgtga gttcccgctc cttcctcccg ttgcggttgg ccagaacaaa gccgctcgac
    15661 tcatcgccga cgaaggccac gaccagaatc aatgggcgtc cctgggcgtc atccgtggcg
    15721 atccagtccc ccgcctgcag ggtccgggct cggctcagcg acttggccca gctccgctgc
    15781 atttcctgtt gcgcggcttc gagttccgca tccgactccg gaagaagcct ggcaatgccc
    15841 agcgcgtccg ccacgccgtc cgccgcaacg ctgacggtct tgacgggtgc cggcttgccg
    15901 tcggccttcg cccgcaacac cttcgcgatg tcgagaagca gaggcttgcg cagacccgga
    15961 tcgaaggaga tgctgatcag gccgtcggca acctgccgca gcagctgctt gaggtcggag
    16021 gcgtccctgc cgccctgctc gggctcggcg acgaactcgg cccacagccg ttcgatgacg
    16081 gcgacccgct cctcccactg agcgcttccc gcacccattc gcaggcaggt atgtaccatc
    16141 aggttgcgcc acccgggatt cagcagcttg agtatcggct ccgggacctc tttgcccgcc
    16201 atcttcggag ccaaggcatt cacgaccgct tttcgagccc gggtcaggcg ttgctgtccc
    16261 tcgctggcgc gcacgatgcg ctccatgttc ccacggtagt tccggatctg ctcatccacc
    16321 agcggattca gctcggcgac cgcctcttca aagatgccgc cgtccccgga gtagccgtct
    16381 gcgacacgct ccacgagcgc atccacgcga ccgctcagct cacgcgcctt cgcttcccgg
    16441 gaatccgcga tgccgctgag acgggcaagc tgattgagca tgatctcggc cgggtggggc
    16501 cggtcgggat cccctccagg gaaaccaggg ttgtgtaccg ccagcttgta gagagtcact
    16561 tccaggcgcc ggacccagtt caagctgcct tggctcaaga accggtcctc acagatggag
    16621 tcgatgaggc tttcgagcac ccgaaaacgg tccaggtcac tctcgccgag gcttctgtcg
    16681 ccgtcggccg cacccagctc ctgcaccagg cgctgacaaa cggtggcccg gtcgccttga
    16741 cctcccggcc ccagcgcgcc cagcacccgg aggatctcct cgctgccgaa cgggttggct
    16801 gcgccgcccc cggctgcctg ccgcggttcg cccccctcgc cttcaagctg ctgcctggcg
    16861 acgcgctcga gtttcacaat gctgcgggca gtggcgtaga cctcgcgcgg cgcggggcca
    16921 acctgctccg cttccggctc atcgtgcgta gccagcttca cgcccaccgc ggcttcctcc
    16981 tcggcgtccg gctcgtcgcc ggaagcctcg ccgtgccggg cggaccccgg tacgtattcg
    17041 tgaagcgtca ggtcgtcgtc gcgggccggc tcctggagag cgctgatcgc ttcgagggct
    17101 tcttccgtag gaaagatctc gcattcggaa agcagctcgc caaggccgtg atagaagtcg
    17161 ttgagcgccg gaagcagcgc cgcctgaaag ccttcgaaga cgtgggttcg gatccgcggg
    17221 gtcagctcca gatccttcag cgcgccgtcg aacgcccagg taatggcagc aggtcccacg
    17281 ggcaccactt ccttgtgccg ccagggcggc tcgacgagcc cgagacgtgc gcgaagatcg
    17341 cgcaggtcgt cccggtagcg gctttcggct ttggcaacga cctcggcgac gtccagccaa
    17401 ccgtcgaact tgtcacttcc ggcctgctcg tgctcacgcc ggatcgcctc ctccctgcgc
    17461 gagcgcaggg gggcaatgtc ctcgagctgc gcgacgttgg atatctgccg aagcacgccg
    17521 ccaatgaagt cgttgcggat ctggttgcgg tacttctcga tcaattcccg gcccgaatcg
    17581 tagatctcgc ccaggccgtc gccgaggctc gccagcgtct tctcgcgcag atcttcctcg
    17641 atcagctcga agcacttctg acagagcgca tcgagggtcg gctgggtgat ccgacgcatc
    17701 ttgcgccgta t
//
LOCUS       DBNHKV010000310         5829 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1389347, whole genome shotgun sequence.
ACCESSION   DBNHKV010000310 DBNHKV010000000
VERSION     DBNHKV010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1389347"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1653)
                     /locus_tag="AC16PP_09770"
     CDS             complement(<1..1653)
                     /locus_tag="AC16PP_09770"
                     /inference="COORDINATES: protein motif:HMM:NF039587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HIR6481957.1"
                     /translation="MTRCRVLMVALALIVAGRSLAESTAGAAQSRSAELNTGEVVDIP
                     YRFHEQASIRVDGRLDDAAWADATVIRDFIVIDPDTMEKPRWETKLMLIYTERGIYAG
                     YDLEQPRDMIVSVLSPRDTGTNFGDFVGLVLDTSGDGKYGYWMSLAASGVKTDGTLIP
                     EAQFNRDWDGVWYGETALTEKGWSAEFFVPWSQLAMPKEAGTRRMSLFASRRSVAIGE
                     RWGVPALPFTITKWIQYMRPLEMEGVDPRQQWSVIPYTSVTQDQVEKETDFRAGAEVF
                     WRPSSNFQVSASVLPDFGNVESDAVIVNLSALETFFPEKRLFFLEGRDIFLTSPRADA
                     SKNFFPVTVVNTRRIGGSPRPPDVPEGVAIPSRQLDQPVELYGAVKATGQLGSLRYGL
                     LGAMEDDVTFKVDDLRFEQSGSEYGVARLLWEDVSYGSYRALGTISTLTAHPEEDAVV
                     HGIDYHYMTGNGSWKLDGQVLYSDKDSVGDGWGTFADAVYTPRRGLKVNFEFEYYDDK
                     LDINDLGFLQRNDITRGGIGVDYTNPSVGWARQLNLSGLSIYE"
     gene            complement(1664..1849)
                     /locus_tag="AC16PP_09775"
     CDS             complement(1664..1849)
                     /locus_tag="AC16PP_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481958.1"
                     /translation="MADVLPFKKRSLKEKAKGRTLCARGFHKWVVDKQQRFDVKQGGL
                     VTVHRCQRCGATKNAAI"
     gene            complement(1974..2330)
                     /locus_tag="AC16PP_09780"
     CDS             complement(1974..2330)
                     /locus_tag="AC16PP_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6481959.1"
                     /translation="MVSIAVVDDDVAILDAMTLLAESRGWLAKVFASGEAFLDAFEHD
                     RDIDCLVLDPHLQGIGGDDVALALADTGVPIVGLTARPESPVTQSILESGAAAILTKP
                     VRPAELVSAIENLLLN"
     gene            complement(2401..3927)
                     /locus_tag="AC16PP_09785"
     CDS             complement(2401..3927)
                     /locus_tag="AC16PP_09785"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding response regulator"
                     /protein_id="HIR6481960.1"
                     /translation="MDFKESHVRDLLDAAPDATVVADSAGVIVFANAQVGEVLGYAPE
                     ALIGLNVDQLLPERLRAAHGAHRADFMSAPRVRPMGAGLDLYGRRRDGSEFPIEISLS
                     PVSTDQGMLVISAIRDVTERKAFEVELSAAKDTAERATAIKSRFLAAASHDLRQPLQS
                     IAIYLDVLNRVVRQDQDKALEVSAKIDKSLDVMRELLDALLDVSKLDSGSITPEVRDF
                     RLQSLFDELVADCAPEAEGKGLTLSIEPTEAVVRTDPALLQRILANFVGNAVRYTEEG
                     SVAVSAAESGDHLLVEVRDTGIGIPGEAIDTIFEEYFQLDNPVRDRRKGLGLGLSIVK
                     QVASLLDCPLEVASTVGEGSCFSILVPLGLLDEEETVPGQPQTGQLAPGEPVILFVDD
                     DPAIVDATTMMLELAGISVRSALDGNRAIAMLEEGLSPDMVVSDFRLPGANGIEVIRR
                     ARELRGSYLPTVLMTGDTSGKVIDEAAISNCTVLRKPVDTNHLISLIERGLDAGRGPG
                     "
     gene            4049..5179
                     /locus_tag="AC16PP_09790"
     CDS             4049..5179
                     /locus_tag="AC16PP_09790"
                     /inference="COORDINATES: protein motif:HMM:NF024874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6481961.1"
                     /translation="MTSRQPVRTMVVDPLRRYPSPMLKLKCSVRKAVLIGALAGASPA
                     MAGAPEHHTESGRFTNPYVEQAQGGVFAFLKRRLLGQDTWSSYDAHRDGAVPTAAPRL
                     VAEGEQTNNARVTWVGHATVLIQHAGVNVLTDPILSRIASPVAFAGPGRKSAPGVDAQ
                     GLPPIDVVVISHDHYDHLDEPTIRFLGDRPRYFVPLGLKQWLVDAGIDQSRITELDWW
                     EKQTVEPGGSALTVTATPTQHVSGRTLTDRNKRLWAGWAMEWDDFTVWFGGDTGYNDV
                     QFKAIGDRFDGFDLGIVPIGSYAPRAYMHSYHADPEQAVRIHQDIRARRSMAVHWGTF
                     HLTAEPLLEPPELLRAAVLEAGLPSEEVTAYAVGESRAYFPL"
     gene            5250..5326
                     /locus_tag="AC16PP_09795"
     tRNA            5250..5326
                     /locus_tag="AC16PP_09795"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5284..5286,aa:Val,seq:cac)
     gene            complement(5383..>5829)
                     /locus_tag="AC16PP_09800"
     CDS             complement(5383..>5829)
                     /locus_tag="AC16PP_09800"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6481962.1"
                     /translation="GEGHIVNTASTAGLQASASIAPYNVAKFGVVALTETLRAELDSE
                     GSAISASVLCPGAINTQIVNSKRNRHSASAHDHQTSDAERAFESRAGRLLAEEGMDPA
                     EVADMVVNAIRGNEFWIVTHPQWIDVLKSRVDAMARDRSLARGFGG"
BASE COUNT         1061 a         1923 c         1801 g         1044 t
ORIGIN      
        1 ctcgtaaatc gacagcccgc tcaggttgag ctgccgggcc cagccgacgc tggggttggt
       61 gtagtcgacg ccgatgccgc cccgggtgat gtcgttgcgc tgcagaaagc ccagatcgtt
      121 gatatccagc ttgtcgtcgt agtactcgaa ttcgaagttg accttgaggc cacgacgcgg
      181 ggtatacacc gcatccgcga acgtgcccca tccgtcaccg acgctgtcct tgtccgagta
      241 aagcacctgg ccgtcgagct tccacgagcc gtttcccgtc atgtagtgat agtcgatccc
      301 atgcacgacg gcgtcctcct cgggatgggc cgtgagagtg gagatggtgc ccagagcccg
      361 gtaacttccg taggaaacgt cttcccagag caggcgggca accccgtatt cgcttccgga
      421 ctgctcgaac ctcaggtcgt ctaccttgaa cgtcacgtcg tcttccatgg cgccgagcag
      481 accgtagcgc aatgagccca actggccggt ggccttgacc gctccgtaaa gttcgacggg
      541 ctggtcgagc tgcctggacg ggatcgcaac cccctccggc acgtcgggcg gccgcggcga
      601 accgccgatg cgccgcgtgt tcaccaccgt gaccggaaaa aagttcttag acgcgtcggc
      661 tctcggcgag gtgagaaaaa tatcgcggcc ctccaggaag aacagccgct tctcggggaa
      721 aaaagtctcc agagccgaaa gattcacgat gaccgcgtcc gactcaacgt tgccgaaatc
      781 gggcaaaacg gatgcggaaa cctggaaatt gctggacggc cgccagaaca cctcggcgcc
      841 ggcccggaaa tcggtctcct tctccacctg atcctgggtc accgaggtgt aaggaatcac
      901 gctccactgc tggcgcgggt ccacgccttc catctccagc ggccgcatgt actggatcca
      961 cttggtgatg gtgaaaggca gggcgggcac gccccagcgc tcgccgattg ccacggagcg
     1021 acgcgacgca aagagcgaca tgcgccgggt gcccgcttcc ttcggcatcg ccagctgcga
     1081 ccagggaacg aagaactccg ccgaccaccc cttttcggtc agggcggttt ctccgtacca
     1141 cactccgtcc cagtcacggt tgaactgagc ctcgggaatg agggtcccgt cggttttgac
     1201 cccgctggcg gcaagggaca tccagtatcc gtacttgccg tcgccggatg tgtcgagaac
     1261 caggccgacg aagtcaccga agttcgtccc cgtgtcccgc ggcgaaagaa ccgagacgat
     1321 catgtcacgc ggctgctcca gatcgtagcc ggcgtagatg ccgcgctcgg tgtagatgag
     1381 catcaacttc gtttcccagc ggggcttttc catggtgtcc gggtcgatga cgatgaagtc
     1441 gcggatgacc gtcgcatccg cccaggccgc gtcgtcgagg cggccgtcca cccggatgga
     1501 cgcctgttcg tggaacctgt aaggaatgtc gacgacctca ccggtattga gctcggcaga
     1561 cctggattgc gcagcgccgg ctgtggattc ggccaggcta cgtcccgcaa cgatcagggc
     1621 taaagccacc atgagaacgc ggcaccgggt catgctgggg cgctcagatg gccgcattct
     1681 tggtcgcgcc gcagcgctgg cagcgatgga cggtcaccag cccgccctgc ttgacgtcga
     1741 agcgctgctg cttgtcgacc acccatttgt ggaatcctct cgcacacaag gtccggcctt
     1801 tggctttctc cttgaggctg cgcttcttga acggcaggac gtcagccatc gcggctcccg
     1861 gattctgcaa aagttgaccg gcccgacaca ccggcatggg gttcgccggg ggcgaattgt
     1921 tgcagctttg caggttcaac gctcgccgat cgttgcccgg cgcagcaagg ccctcaattc
     1981 agcaacaggt tctcgatggc acttaccagc tcggcaggcc tgaccggctt ggtgaggatg
     2041 gctgccgccc cactctcgag aatcgattga gtcacggggc tctccggtcg agcggtcagg
     2101 ccgacgatgg gtacgcccgt atccgccagc gccagggcga cgtcgtcgcc gccgataccc
     2161 tgcaggtggg ggtcgaggac caggcagtcg atgtcccggt cgtgctcgaa cgcgtcgaga
     2221 aaagcctctc cgctggcaaa gactttggcc agccagcccc gactctcagc gagcagggtc
     2281 atggcatcca gaatggccac atcatcgtct accaccgcaa tggaaaccat cgcccaccgt
     2341 ccgcatttgc cttttagcga ggttaaggcg ggcagctgtg gcctcaattc gggaatctac
     2401 ctagccgggc ccacgtccgg cgtcgagtcc ccgctcgatg agactgatga ggtgattggt
     2461 gtcgacgggt ttgcgcagga cggtgcagtt ggatatcgcg gcctcgtcga tgactttgcc
     2521 ggaggtatca ccggtcatca gcaccgtcgg cagatagctg ccccgcagct cgcgcgcgcg
     2581 ccggatgact tcgatgccgt tggcgcccgg caggcggaag tcggacacca ccatgtcggg
     2641 agacagcccc tcctccagca tggcgatggc cctgttgccg tcgagcgccg agcgcaccga
     2701 gatcccggcg agttccagca tcatcgtcgt cgcgtccacg atggcggggt catcgtctac
     2761 gaagaggatc accggttcgc caggagccaa ctggccggtc tgcggttggc ccgggaccgt
     2821 ctcctcctcg tccagcagcc ccaggggcac cagaatgctg aaacatgagc cctcgcccac
     2881 ggtggaagcc acctccagcg ggcagtcgag caagctggct acctgtttca caatggacag
     2941 accgagaccc agccctttgc gccggtcgcg gaccggattg tcgagctgga aatactcctc
     3001 gaagatggtg tcgatggctt cccccggaat gccgataccc gtgtcgcgca cctcgacgag
     3061 caggtggtct ccactctcgg ccgcggagac ggcgacgctg ccttcctccg tataacgaac
     3121 cgcattgccc acgaagttgg ccaggatccg ctgcagcagc gctggatccg tgcgaacgac
     3181 ggcctcggtg ggctcaatgg atagcgtgag gcctttgccc tccgcctcgg gagcacagtc
     3241 tgcgaccagc tcgtcgaaca gactctgaag ccggaaatcc cgcacctctg gcgtaatcga
     3301 gcccgagtcc agcttggaga cgtccagaag cgcatcgagc agctcacgca tcacgtccag
     3361 agacttgtct atcttggcgc tgacttccaa cgccttgtcc tggtcctgac gcaccacccg
     3421 gttgagcacg tcgagataga tggcgatgga ttgcaggggt tgccgcagat catggctggc
     3481 agctgccagg aacctggact tgatcgccgt ggcgcgctcc gcggtgtcct tggcggcact
     3541 cagctcgacc tcgaaagcct ttctttccgt gacgtcgcgt atggcgctga tgaccagcat
     3601 accctggtcc gtgctgacag ggctcaagct gatctcgatg ggaaactccg agccgtcccg
     3661 acgacgcccg tagaggtcga gcccggcacc catgggccgg acccgcggcg cagacatgaa
     3721 gtcggcgcga tgtgcgccat gggctgcacg caaccgctcg ggcagcagct ggtcgacgtt
     3781 cagaccgatg agggcttccg gcgcgtaacc gagcacctcg ccgacctgcg cattcgcaaa
     3841 gacgattacg ccagccgaat ccgccaccac ggtcgcgtcc ggcgctgcat cgagcagatc
     3901 acggacgtga ctttccttga aatccatcgt ctccccctca aattacgcta tcagaaacgc
     3961 ggcgagggga gaagcgccac atggggcctg aattcaagcc tccggggtgc ccggagaagg
     4021 aaccaaatcc tggctggacg gtccaaatat gacttcccgt caaccggtcc gaaccatggt
     4081 cgtcgatccg ctgaggcgct accctagccc catgctgaag ctcaaatgct ccgtgcgtaa
     4141 ggccgtgctg atcggcgcgc tggccggtgc gagccccgcc atggcgggcg cgccggaaca
     4201 ccacaccgaa agcggccgtt tcaccaaccc ctacgtggaa caagcccaag ggggcgtttt
     4261 tgcgttcctt aaacggcgac ttttaggaca ggacacgtgg tccagctacg acgcccatcg
     4321 cgatggcgca gtgcccacgg ctgcaccccg cctggtcgcc gagggcgagc agaccaacaa
     4381 cgccagggtg acctgggtcg gtcatgccac cgtgctgatt cagcacgccg gcgtcaacgt
     4441 gctcacggac cccatacttt cgcgaatcgc gtcgcccgtc gcgttcgcag ggccgggtcg
     4501 taagtcggcc ccgggcgtag acgcccaggg ccttcccccc atcgacgtgg tggtcatctc
     4561 ccacgatcac tacgaccacc tcgacgagcc tacgatccgc tttctgggcg accggccccg
     4621 atacttcgtt cctctgggac tgaagcagtg gctggtggat gccggtatcg atcaatcgcg
     4681 aatcaccgag ctggactggt gggagaagca gacggtcgag cccggcggct ccgcgttgac
     4741 ggtgaccgcc acccctaccc agcacgtctc gggacggacc ctgaccgatc gaaacaagcg
     4801 gctgtgggcc ggctgggcaa tggagtggga cgactttacc gtctggtttg gcggggatac
     4861 cggatacaac gacgttcagt tcaaggccat cggcgatcgt ttcgacggct tcgacctcgg
     4921 catcgtaccc atcggcagct acgccccgcg ggcgtacatg cacagctacc acgccgaccc
     4981 tgagcaggca gtgcgcatcc atcaggacat ccgcgcgagg cggtccatgg ccgtgcactg
     5041 gggcaccttc cacctcactg ccgagccgct gctcgagccc cccgagctgc tgcgcgcagc
     5101 cgttctggag gcggggctgc ccagcgaaga ggtcaccgcc tacgcggtgg gcgaatcccg
     5161 cgcctacttc cccctctgaa cagttcccag ctgcacccga gcggcgaagc ggtagcttcg
     5221 gccgccgttt ttggtaggct agcttcgcag ggcgcgtagc tcagttggtt agagtgctgc
     5281 tttcacacgg cagaggtccg cagttcgaat ctgcgcgcgc ccaccatctc gcccgggttg
     5341 ccggccgaac cgtgcgctat gcaggccggc gccaccgctg gctcaacctc caaaacccct
     5401 ggcaagggac cgatctcggg ccatggcgtc cactcgcgac ttgagcacgt cgatccactg
     5461 ggggtgcgtg acgatccaga actcgtttcc ccggatggcg ttgaccacca tgtcggctac
     5521 ctccgcagga tccatgccct cttccgccag cagccgtccg gcccggctct caaaagctcg
     5581 ctccgcatcc gaggtctggt gatcgtgcgc cgaagcgcta tggcggtttc gtttgctgtt
     5641 gacgatctgg gtattgatgg cgccgggaca gagcacggaa gctgagatgg cggagccttc
     5701 ggagtcgagc tccgcgcgca gggtttctgt caacgcaacc acgccgaact tggcaacgtt
     5761 gtagggcgca atgctcgccg aggcctgcag gcctgcggtg gatgcggtat tcacgatgtg
     5821 cccctcgcc
//
LOCUS       DBNHKV010000311        10108 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1248947, whole genome shotgun sequence.
ACCESSION   DBNHKV010000311 DBNHKV010000000
VERSION     DBNHKV010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10108
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1248947"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="AC16PP_09805"
     CDS             <1..1052
                     /locus_tag="AC16PP_09805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase family protein"
                     /protein_id="HIR6481963.1"
                     /translation="LSARSIVLATGAEPVVPPIPGLADARPLTSDNLWELESLPQRLL
                     VMGGGPIGCELAQAFARLGSAVTLIDMAPGLMPREDDDVAGFVERRLRGEGIDVRTGH
                     RAVRVVASADGAGVLHADSDGGEVQIGFDRVLVAVGRRANTAGLGLEALGIATRSDGT
                     LEVDAYLRTAVRNVYGCGDVVGPYQFTHMASHQAWYAAVNALFGRFRKFKVDYSVVPW
                     VTFTDPEVAHVGLSETEAAERGIRVEVTRYGLDDLDRALADGSASGWVKILTRPGSDR
                     ILGVSIVGAHAGELIAEYVLAMTHGIGLKKILGTIHVYPTLAEANKLAAGAWRRAHAP
                     ERLLRWVGRLHDLLR"
     gene            complement(1049..1651)
                     /locus_tag="AC16PP_09810"
     CDS             complement(1049..1651)
                     /locus_tag="AC16PP_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6481964.1"
                     /translation="MTDRTFCYPHFVGNNDKPAANSRAELENRLARVFIEHGYEGASL
                     SRLATASGLGKASLYHHFPGGKAEMASVLLTRAIARLDKTAFSRLTKKQPAPVRLGRF
                     VDAFSSYVDHGERPCLIAVLDHGALRAVHGDTIRRRFLSWGERLAATFEETGMKPRRA
                     RRAASDLLAGLYGNLAMARMLDDASLFQRGTRRLKKDLPS"
     gene            1798..3075
                     /locus_tag="AC16PP_09815"
     CDS             1798..3075
                     /locus_tag="AC16PP_09815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6481965.1"
                     /translation="MKFGIFYEHQLPRPWDDGAEQRLFNEALDQVELADRLGIDYAWE
                     VEHHFLEEYSHSSAPEVFLAACSQRTKNIRLGQGIRQVIPQYNHPARTAEVIATLDLV
                     SQGRVEFGTGESSAELELGGFRIPVAKKRKIFMEATEQICNMLAMDPYPGYKGQYFEM
                     PCRNVVPKPVQRPHPPVWVACSQRETIRMAARLGIGALTFAFVDPMEAQSWVDEYYGI
                     IKSDQCVPIGHAVNANICMVSSFSCHHDRQTAVQRGLEGFEFFGYALGSLYGFGEHKP
                     GRTDLWTEFQTVRAPQVRNEVADFSEALLGARGGIGTPEDLRQHLRKFERCGVDQVTF
                     IQQAGMNRHEHICESLEIFAAQVMGEFKEREAEREERKMRELEPYIAAAMERKVRMKP
                     LADDEIPTFPALGRRITEEQEDAPSIYERPAAG"
     gene            3134..3730
                     /locus_tag="AC16PP_09820"
     CDS             3134..3730
                     /locus_tag="AC16PP_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6481966.1"
                     /translation="MASFAEIRLEPGVARGLKRGDRSAQGIAYRVLAPTVMGLACRIL
                     QDEGSAQEVVQDTFVDLIEHARTLKDAQAVVGWVRQVAVNHCLMRLRSPWNKRRDELD
                     ADPAGDVYVDMAEGAARTEGMQDIEKALSELEPETRLVIWLHDVEGYTHQEIAGLVGR
                     TASYSKSQMARGYQKLAKRFRGTEDGIENTASRSARVP"
     gene            3687..4304
                     /locus_tag="AC16PP_09825"
     CDS             3687..4304
                     /locus_tag="AC16PP_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481967.1"
                     /translation="MESKTPLADLLAYRDGEELPADVARGIEGDPAAQGELEKLRGIR
                     QQLNALPAVLPDPAVWEEISRRAQPPRSWTLRYPLATAATVFLAAALTIVAWNPLGNG
                     GAGAGPEPVVVADGALVDLMSRSQALESHLPVQGTPAGQLAWGSAEEALRYRIADIDA
                     QLMRIYETEPVDVERREALWRQRVDLLESLTQVQRGQAVVRPAIY"
     gene            4314..5405
                     /locus_tag="AC16PP_09830"
     CDS             4314..5405
                     /locus_tag="AC16PP_09830"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR6481968.1"
                     /translation="MIKWLSSRKRQVLAGFTAMALLLSGYTFADELEDRLREAREQLD
                     AAAERLAELHRQMYEAEVSEGRYGKRAMIGILLTSMGDDDGLVIAGVTPDGGAKQAGL
                     AAGDRIVAIGGVRLDTGGADKPGKLLSNVVKDLEPGDPVKVEYVRDGETLKADVVTQP
                     KRAFVMKSLAMELDQLDPEFTAELIKEFEDLGELKELKELGKLEALHELGDLGELIGN
                     VVDVGTSLKLEDMDGDLARYFGVDAGVLVVAPPSQEGELKSGDVLLELDGKDVANARE
                     AMKTLAMSDGTIDAGVLRNGKKREVSIDCSQFSAGDHMAITIDPSIIRIRRGIDGDDG
                     VDVRIVVDEEVVVDEKGSKKAAADDSAGG"
     gene            complement(5444..7324)
                     /locus_tag="AC16PP_09835"
     CDS             complement(5444..7324)
                     /locus_tag="AC16PP_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003161181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR6481969.1"
                     /translation="MLQLIDISLKRGSNQLFEHLTCTIHPGHKVGMVGRNGAGKSTLF
                     DMILGRLQPDEGEVSRPSGWVASHMAQEVNVTDRPAIEHVIDGHRELRAVERRIAQAE
                     SRRDDLTLAELHTRFGDVGGHQARARAGTILHGLGFAADEIEQPFRAFSGGMRIRLNL
                     AQALMAPADLLLLDEPTNHLDLETTVWLESWLQRFAGTLLVIAHDRAFLDGVCDHIVH
                     IEHGRANSYRGNYSSFERQRAEALAQQQAAHARQQAEIKHMQHFIDRFRAKASKAKQA
                     QSRMRALERMQAVAPVHADSPYRFSFANPEQMSHPLLSLEDVSVGYEGRAVIEHVRQT
                     LLPGARIGILGANGAGKSTLLKCLVGELEPLAGTLVRGRHSAVGYFAQHQLERLDGQT
                     SALATLTNERPQAREQWCRDYLGRWGFPADMVERPIVTLSGGEKARLVLALIALAQPA
                     ILVLDEPTNHLDLEMREALALALQDYAGALLLVSHDRSLLRRAVDEFWLVEGGRLASF
                     AGDIDAYAESRLRRSEPPSSERAPETNRKALRQAAAAQREAAKPLRAKLRHLEKEMDQ
                     IGVELREVESRLADGDVYRSLPQEELDDLLSAAGRLRKRLDATEQAWLSATEALEALE
                     QS"
     gene            complement(7426..8178)
                     /locus_tag="AC16PP_09840"
     CDS             complement(7426..8178)
                     /locus_tag="AC16PP_09840"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HIR6481970.1"
                     /translation="MNANVRRILISNGKGGCGKTTIAANLAVSYANRGAKVALVDQDP
                     QASSSYWADQRSRELAPVHLVRAHQRANMYQTQTFHNRIPADISRVVVDGSTNPQDRE
                     LEMLLKQCDVILVPLLPSAIDIRAGSQFIKELITHRSFRTLPKPVGVIANRVQPNSTM
                     HARLEHFLKCLDVPWVATFRDSPLYAEAFERGEGVVDLIDCRAARREAPAWRALTAWI
                     ERQPVSRGCTARELRNRPIRAPRHVDDETLSA"
     gene            complement(8438..9628)
                     /locus_tag="AC16PP_09845"
     CDS             complement(8438..9628)
                     /locus_tag="AC16PP_09845"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6481971.1"
                     /translation="MSRAQLINMARHNIGHAKAGTIEQAPDIVKVPVQNYFDPDRWQL
                     EMDRIFRRMPLVLAMTDELRNPGDYKAMEAVGMPVLLSRGEDGEVRAFVNMCRHRGSQ
                     VMLPGCGNAHRFTCPYHAWTYDQEGALVGVYAPKDFGDVDKGEHGLVPLPVAERAGMI
                     WVIVNPQATLDIDTFLSGYDELLGHFHFEDWHLFEQRTIAGPNWKIAYDGYMDLYHLP
                     ILHKDTFGDQFPNQALYYAWGPHQRVSSPDPTLAGYEQLPESEWNEAHMLAGVWTIFP
                     HVSVAGFDGGGRSVMVSQLFPGKTPQESVTVQNYLMEKAPDAEQAEAAEQMFKLLEYV
                     VQEEDYATGLRQQKAIMTGAMSHVMFGRNEEGGQRFHRWLDTLLATDDEDLNALFAGG
                     ERYC"
     gene            complement(9723..>10108)
                     /locus_tag="AC16PP_09850"
     CDS             complement(9723..>10108)
                     /locus_tag="AC16PP_09850"
                     /inference="COORDINATES: protein motif:HMM:NF014639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HIR6481972.1"
                     /translation="PCPLCLMQRIWFFVAGAFAFASLLHNPRLGIYPLCSMVAAVIGG
                     GFSIRQLYLQGLPPDQVPSCGPDMSYMLEAFPLTDVLIAMTRGTGDCAEVAWSFLGIS
                     IPGWALVGFAALAVMAALQLRAGTR"
BASE COUNT         1676 a         3251 c         3458 g         1723 t
ORIGIN      
        1 gcctgagcgc ccgcagcatc gttctcgcca ccggggctga gcccgttgtc ccgcccattc
       61 ccgggctcgc cgatgcccgg ccgctcacct ccgataatct ctgggagctc gagagcctgc
      121 cccagcggct tctggtgatg ggtggagggc cgatcggctg cgagctggct caggctttcg
      181 cccgactggg ctcggcggtc acgctcatcg acatggcgcc cggcctgatg ccccgggagg
      241 acgacgacgt cgccggcttc gtcgagcgca ggctgcgcgg tgagggcatc gacgtccgca
      301 ccggccaccg cgccgtgcgg gtggtggcct cggcggatgg cgccggggtg ctacatgccg
      361 attcggacgg cggcgaggtg cagatcggct tcgatcgggt gctcgttgcc gtcgggcgcc
      421 gcgccaacac ggcggggctg gggctcgaag cgctggggat cgccacccgg tcagacggaa
      481 cgctggaggt ggacgcctat ctccgcacgg cggtgcgtaa cgtctacggc tgcggcgacg
      541 tggtgggtcc ctaccagttc actcacatgg cctctcatca ggcctggtac gcggcggtca
      601 acgcgctgtt cggccggttc cgcaagttca aggtcgacta ctcggtggtg ccctgggtga
      661 cgttcacgga tccggaagtc gcccacgtcg ggctctcgga aaccgaggcc gccgagcgag
      721 gcatccgggt cgaggtcact cggtacggtc tggacgatct ggatcgggcg ctcgccgacg
      781 gcagcgcctc gggttgggtg aagatcctta ccagacccgg ctcggatcgc attctgggcg
      841 tttccatcgt gggagcccat gcgggcgagc tcatcgccga gtacgtgctg gccatgaccc
      901 atggcatcgg gctgaagaag attctcggca ccattcacgt ctacccgacc ctcgccgagg
      961 cgaacaagct cgctgcgggg gcatggcggc gcgcccacgc gcccgaacgg ctgctacgct
     1021 gggtcggcag gctccacgac ctgttgcgct aggaaggaag gtccttcttc agtcgcctgg
     1081 tgccgcgctg gaacagcgac gcatcgtcca gcatcctggc cattgccagg ttcccgtaca
     1141 gtccggccag cagatcggaa gccgcccgac gcgcccggcg cggtttcatg ccggtttcct
     1201 cgaacgtggc ggcaagccgt tcgccccagc tcagaaaccg ccggcggatg gtgtcaccgt
     1261 gcacggcccg caacgcgccg tggtccagca ctgcgatcag acaggggcgc tcgccgtgat
     1321 ccacgtagct gctgaaagcg tccacgaacc ggccgagacg aaccggggca ggctgcttct
     1381 tggtcagccg ggaaaaggcc gttttgtcca gccgggcgat cgccctggtc aggagcaccg
     1441 acgccatctc cgccttgccg ccgggaaagt gatggtagag gctggctttc cccagcccgc
     1501 tggcggtcgc caggcggctc aagctggcgc cctcgtagcc gtgctcgatg aagactctgg
     1561 cgagccgatt ttcgagctcg gcgcgactat tcgcagctgg cttgtcgttg ttgcccacga
     1621 aatgagggta acagaacgtt cggtcggtca aaaggcgccg tctgatccgc gccgctggcg
     1681 cggtgcccct tgatgcgacg tgccctttga ggggagactc gggcagcggg aaagcgccat
     1741 tcggggccct ggccgcccgg ccgccgggga tctgcacaac acagtgggga catgaacatg
     1801 aaatttggga tcttctacga gcatcagctg ccgcggccct gggacgacgg cgcggagcag
     1861 cggctgttca acgaggcgtt ggaccaggtg gaactcgcgg acaggctggg catcgactat
     1921 gcctgggagg tcgagcacca ctttctcgag gagtattccc actcctcggc gcccgaggtc
     1981 ttcctggcgg cgtgttccca gcgcaccaag aacatccgcc tgggtcaggg cattcggcag
     2041 gtgatcccgc aatacaatca tccggcccgc acggccgagg tgatcgccac cctggatctc
     2101 gtctcccagg gccgcgtgga gttcggcacg ggcgagtcct cggcggagct cgagctcggc
     2161 ggcttccgga ttcccgtggc caagaagcgc aagatcttca tggaggccac cgagcagatc
     2221 tgcaacatgc tcgccatgga cccgtatccc gggtacaagg gccagtattt cgagatgccc
     2281 tgccggaacg tggttcccaa gcccgtgcag cggccccatc cgcccgtctg ggtggcctgc
     2341 tctcagcgcg agaccatccg gatggccgcc cggctgggca tcggggcgct gaccttcgcc
     2401 ttcgtcgacc ccatggaggc tcagtcctgg gtcgacgagt actacggcat catcaagagc
     2461 gatcagtgcg tgcccatcgg tcacgccgtc aacgccaaca tctgcatggt gtccagcttc
     2521 tcctgtcacc acgaccgcca aaccgccgtg cagcgcggcc tggaaggctt cgagttcttc
     2581 ggctacgccc ttggcagcct ctacggcttt ggcgagcaca aaccgggccg cacggatctc
     2641 tggacggagt tccagaccgt gcgcgcgcct caggtcagga acgaggtggc cgacttcagc
     2701 gaggctctgc tgggcgccag aggcggtatc ggtacccctg aggatctgcg ccagcatctg
     2761 cgcaagttcg agcgctgcgg cgtggatcag gtgaccttca tccagcaggc cggaatgaac
     2821 cggcacgagc acatctgcga gtctctcgag atcttcgctg cgcaggtcat gggagagttc
     2881 aaggagcggg aagcggagcg ggaggagcgc aagatgcgtg agctcgagcc ttacatcgct
     2941 gccgccatgg agcgcaaggt gaggatgaag ccgctcgcgg acgacgagat accgactttc
     3001 ccggcgctcg gacggcggat caccgaggaa caggaggacg ccccgtccat ctacgagcgc
     3061 ccggcggccg ggtagccgaa aaaaatctcc gttgctgcga ccctttgcgg gtctgacgca
     3121 tctatagagt ggtatggcca gttttgccga aattcgcctg gagccaggcg ttgcccgggg
     3181 cctcaagcgg ggtgaccgca gtgcccaggg catcgcctac cgtgtgctcg cgcccactgt
     3241 gatgggcctc gcctgccgca ttctccagga cgagggatcg gctcaggaag tcgtgcagga
     3301 caccttcgtg gatctgatcg agcacgcgcg cacgctcaag gacgcgcagg cggtcgtcgg
     3361 ctgggtgcgg caggttgccg taaaccactg cctgatgcgg ctgcgttccc cctggaacaa
     3421 gcggcgggac gagctcgatg cggacccggc aggggacgta tacgtcgaca tggccgaagg
     3481 ggcggcccga acggagggca tgcaggacat agagaaagcg ctgagcgagc tggagccgga
     3541 gacccggctg gtgatctggc tccacgatgt ggagggctat acccaccagg agatcgcagg
     3601 gctggtgggc cgcaccgcca gctactcgaa gtcccagatg gcgcgaggct accagaagct
     3661 cgcgaagaga ttcagaggaa cggaagatgg aatcgaaaac accgctagcc gatctgctcg
     3721 cgtaccgtga cggcgaggag ttgcccgccg acgtggcgcg cggcatcgaa ggcgaccctg
     3781 ccgcccaggg cgagctggag aagctgcgtg gcataaggca gcagctcaac gcgctcccag
     3841 ccgttcttcc cgatccggcg gtgtgggagg agatcagccg cagggcgcaa ccgccgcgca
     3901 gctggacgct gcgctacccc ctggcgaccg cggcaacggt ctttctggcg gcggccctca
     3961 ccatcgtcgc ctggaacccc cttggcaacg gcggagccgg cgccggtcca gagcccgtcg
     4021 tggtggccga tggtgccctg gttgacctga tgagccgatc ccaggctctc gagtcgcacc
     4081 tgcccgtgca gggcacgccc gccggtcagc tggcctgggg ctctgccgag gaggcgctca
     4141 ggtaccggat tgccgatata gatgcgcagc tcatgcggat ctacgagacc gaaccggtgg
     4201 acgtcgagcg tcgtgaagcg ctgtggcggc agagggtcga cctgctcgaa tcgcttactc
     4261 aggtgcagcg cggccaggcc gtggtgcgac ctgcaattta ctaggagtcg atgatgatca
     4321 agtggttgag ctcgcggaag cggcaggtgc tcgccgggtt cacggcgatg gcactgctgc
     4381 tctccggata tacctttgcc gacgagctgg aggaccggct gcgcgaggcc cgcgaacagc
     4441 tcgatgccgc cgccgagcgg ctcgccgagc tgcatcggca gatgtatgaa gccgaggttt
     4501 cagagggtcg ctacgggaaa cgcgcgatga tcggtatcct tctgacgagc atgggcgacg
     4561 acgacggcct ggtgattgct ggcgtgacgc ccgatggcgg cgcgaagcag gctggactgg
     4621 ccgcgggcga tcggatcgtc gccatcggcg gcgtgcggct ggacaccggc ggtgcggaca
     4681 aacccggcaa gctcctcagc aacgtggtca aggacctcga gcccggcgac cccgtgaagg
     4741 tggaatacgt gcgcgacggc gagacgctga aagccgacgt ggtcacgcag cccaaacgtg
     4801 ccttcgtgat gaagtccctg gcgatggagc tggatcagct cgatcccgag ttcacggccg
     4861 agctcatcaa agagttcgag gacctcggag agctcaagga actcaaagaa ctcggcaagc
     4921 tggaggctct tcacgagctc ggagatctgg gcgagctgat cggcaacgtc gtcgacgtgg
     4981 gcacctcctt gaagctcgaa gacatggacg gcgacctggc caggtatttc ggagtcgacg
     5041 caggcgtcct cgtggtcgct ccgccttcac aggaaggcga gctcaagagt ggggacgtgc
     5101 tgctcgagct cgacggcaag gacgtggcca acgcccggga agccatgaag acgctcgcca
     5161 tgagtgacgg gaccatcgac gccggggttc tgcgcaacgg caagaaacgc gaggtgtcca
     5221 tcgactgctc gcagttctcc gcgggcgatc acatggccat cacgatcgat ccgagcatca
     5281 tccggattcg gcgtggcatc gacggcgacg atggagtcga cgttcggatc gtcgtggacg
     5341 aggaggtggt cgtggacgag aaaggctcta aaaaagcagc cgccgacgat tcggcgggcg
     5401 gctaggcgtc cggaccgacc ctcatcccct ccgggcgcgc cgttcagctc tgctcgagcg
     5461 cttcgagcgc ctcggttgcg ctgagccagg cctgctcggt cgcgtccaat ctttttctca
     5521 gcctgccggc agcggagagc aggtcgtcga gctcctcctg aggcaggctc cggtacacgt
     5581 ccccatccgc cagtcgggac tccacctcgc gcagctccac gccgatctga tccatctcct
     5641 tttccaggtg cctgagcttt gcccgcaacg gcttggccgc ctcccgctgg gcggccgccg
     5701 cctgtcgcag ggctttgcgg ttcgtctcgg gagcgcgttc ggatgacgga ggttcgcttc
     5761 gcctcagacg gctctcggcg taggcgtcga tatcgccggc gaaactcgca agcctgcccc
     5821 cctcaaccag ccagaactcg tccacggccc gccgcagcag gctgcgatcg tgggagacca
     5881 gcagcagggc gcccgcatag tcctgcaacg ccagggccag ggcctcgcgc atttccaggt
     5941 ccaggtggtt ggtcggctcg tcgaggacga gaatggccgg ctgggcgaga gcgatcaggg
     6001 ccagcacgag acgggctttc tcgccgccgg aaagcgtaac gatgggccgc tccaccatgt
     6061 cggcggggaa gccccagcgg cccaggtagt cccggcacca ttgctcccgg gcctgtggcc
     6121 gctcgttcgt gagggtcgcc agagcggacg tctgcccgtc cagccgttcg agctgatgct
     6181 gggcgaagta cccgaccgcg gagtggcgtc cgcgcaccag cgtgcccgcc aggggctcga
     6241 gctcgcccac cagacacttg agcagggtgg acttgccggc gccgttggct cccagtatgc
     6301 cgatgcgggc gccgggcaga agtgtctgcc tgacgtgctc gatcaccgcg cgtccttcgt
     6361 agcccacgga cacgtcctcc aggctcagca gggggtggga catctgctcc ggattcgcga
     6421 agctgaagcg gtagggcgag tcggcatgca cgggggctac ggcctgcatg cgctccaggg
     6481 ccctcattcg gctctgtgcc tgcttcgctt tgctggcctt ggcgcgaaac cggtcgatga
     6541 aatgctgcat gtgcttgatc tcggcctgct gcctggcgtg ggccgcctgc tgctgcgcca
     6601 gcgcttccgc tcgttgccgc tcgaacgagg agtagttgcc ccggtaggag ttcgcgcgcc
     6661 cgtgttcgat gtgaacgatg tggtcgcaca cgccgtcgag gaaggcccga tcgtgcgcga
     6721 tcacgagcag ggttccagca aagcgctgca gccaggattc cagccagacc gtggtctcca
     6781 ggtccaggtg gttggtaggc tcgtccagaa gcagcagatc ggccggagcc atcagcgcct
     6841 gggccagatt caggcggatc cgcatgccgc cggagaaagc tcgaaacggc tgctcgatct
     6901 catcggcggc aaagccgagc ccgtgcagga tagtcccggc gcgggcccgt gcctggtgcc
     6961 cgccgacgtc gccgaagcgc gtatgtagct ccgccagcgt caggtcatcg cgccgggact
     7021 cggcctgtgc gatgcggcgc tccacggctc ggagctcccg gtggccgtcg atcacgtgct
     7081 cgatggccgg ccgatcggtg acgttgactt cctgggccat gtgggaggct acccagccag
     7141 aggggcggct cacctctccc tcgtcgggct gcaggcggcc gaggatcatg tcgaacagag
     7201 ttgatttgcc cgcgccgttg cgcccgacca tgccgacctt gtggcctggg tggatcgtgc
     7261 acgtcaggtg ctcgaacagc tggtttgagc cgcgcttcaa ggaaatgtcg atcagctgga
     7321 gcatcggtgg ggcggctctg tgaaaatgac gcgcagaacg tgatcagatg aggtggcgcc
     7381 gggcctgatt ttcggaagac ccgagaagct ggctggcgcc ggattctatg cgctcaaggt
     7441 ctcgtcgtcg acgtggcgcg gcgcccggat cggtcggttg cgcagctccc gggccgtgca
     7501 acctcggcta acgggctgcc gctcgatcca ggccgtgagc gctcgccacg cgggcgcttc
     7561 ccggcgcgcg gcccggcagt cgatcaggtc cacgacgccc tctccccgct cgaaggcttc
     7621 cgcatagagg gggctgtcac ggaaggtggc gacccacgga acgtccaggc acttgaggaa
     7681 gtgctccagc cgcgcgtgca tggtggagtt cggctgaacc cggttggcga tgacgcccac
     7741 gggcttgggc agcgtacgga agctccggtg ggtgatcagc tccttgatga actggctgcc
     7801 ggctcgaatg tcgatggccg acggcagcag cggaaccaga atcacgtcgc actgcttcag
     7861 gagcatctcc agttctctgt cctgtgggtt ggtgctgccg tcgacgacga cccggctgat
     7921 gtcggcgggt atccggttgt gaaaggtctg agtctgatac atgttggcgc gctgatgcgc
     7981 gcggacgagg tgaacgggtg caagctcccg ggagcgctgg tccgcccaat agctgctgga
     8041 cgcctgcgga tcctggtcga ccagtgcgac cttggcgccg cgattggcgt atgacacggc
     8101 aagattggcg gcgatggtgg tcttgccgca cccgcctttg ccgttgctga tcagtatgcg
     8161 ccgaacgttc gcattcatgc gctggggccc tttctttgag agctctctgc gaatcgggat
     8221 ccctgtgtcc ctgggcctcg tttcaggccc cccctgctcc atgcgtcccc caggacgccg
     8281 gcgtcgaccg ttgtctggcg gccgggccgg acgggcagcg ctggaatata acaccgtttg
     8341 cgcggatgtc accaatggtg cagggcccct ggcgccgcat gacgcgcctc tcgccggggc
     8401 ccttcggcgc ccgggaggcc gtccgggaag cccgcggtca gcagtaacgc tcgccgcccg
     8461 caaacagggc gttgaggtcc tcgtcatcgg ttgcgagcag ggtgtccagc cagcggtgga
     8521 agcgctgccc gccctcttcg ttccggccga acatcacgtg gctcatggcg ccggtcatga
     8581 ttgctttctg ctgccgcagg ccggtggcgt agtcctcctc ctgcaccacg tactcgagca
     8641 gcttgaacat ctgctcggcc gcctcagcct gctcggcgtc gggcgccttt tccatcaggt
     8701 agttctgcac ggtcaccgat tcctggggcg tcttgcccgg gaacagctgg gacaccatga
     8761 cgctgcggcc gccgccgtcg aagccggcga cggagacgtg cggaaagatg gtccacacgc
     8821 ccgccagcat gtgtgcttcg ttccactcgg actccggcag ctgctcgtag cctgcaagcg
     8881 tcgggtccgg cgagctgacc cgctgatggg gcccccaggc gtagtacagc gcctggttgg
     8941 gaaactgatc accgaacgtg tccttgtgca ggatcggcag gtggtagagg tccatgtagc
     9001 cgtcgtaggc gatcttccag ttggggccgg cgatcgtgcg ctgctcgaag agatgccagt
     9061 cctcgaagtg aaagtgcccg agcagctcgt cgtatccgga caggaaggtg tcgatgtcca
     9121 gggtcgcctg gggattgacg atgacccaga tcatgcccgc ccgctcggcg acgggaaggg
     9181 gcacgaggcc gtgttccccc ttgtcgacgt cgccgaaatc cttcggcgcg tagacgccga
     9241 ccagagctcc ctcctggtcg taggtccatg catggtaggg acaggtgaaa cgatgagcgt
     9301 tgccgcatcc gggcagcatc acctgggatc cgcgatgtcg gcacatgttg acgaatgccc
     9361 ggacctcgcc gtcctcgccg cggctcaaga gcacgggcat gcccaccgct tccatggctt
     9421 tgtagtcgcc cggattgcgc agctcgtccg tcatcgccag caccagcggc atgcggcgga
     9481 agatgcgatc catctccagc tgccagcgat cggggtcgaa gtagttctgc accggcacct
     9541 tgacgatgtc cggagcctgc tcgatggttc cggctttcgc gtggccgatg ttgtgcctgg
     9601 ccatgttgat cagctgcgcc cgactcattc tgcgttctcc taaagcgagg ctcacagcat
     9661 agctcacctc gggcaatcaa caagcgccgc ctgctcagcg gcaggtggct ccctgggacc
     9721 ggtcagcgtg ttcctgcgcg cagctgcagc gccgccatca ccgccagagc ggcaaacccc
     9781 accagggccc agccgggaat ggaaattccc aggaaactcc aggcgacctc ggcgcaatcg
     9841 cccgttcccc gggtcatggc gatcagcacg tcggtgaggg ggaaggcctc gagcatgtag
     9901 ctcatgtccg ggccgcagga cggcacctgg tccggcggga ggccctggag gtacagctgc
     9961 cggatcgaga acccgccgcc gatcaccgcc gcgaccatgg agcacagcgg gtagatcccc
    10021 agccgcgggt tgtgcagcag gctggcgaac gcgaaggcgc cggcaacgaa gaaccagatt
    10081 cgctgcatca ggcagagtgg gcatggcg
//
LOCUS       DBNHKV010000312         8363 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1746318, whole genome shotgun sequence.
ACCESSION   DBNHKV010000312 DBNHKV010000000
VERSION     DBNHKV010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8363)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8363
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1746318"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="AC16PP_09855"
     CDS             complement(<1..1318)
                     /locus_tag="AC16PP_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012861.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIR6481973.1"
                     /translation="MIRFTSSSSAPSAEIGALRLPNKVSPEFFRNRLADIEALPQLAL
                     LAVLAGLITGCVILLFRAAVELILGGWLLPEGSESFEALSILERLALPIAGAGLIGLA
                     LNRLSAAERRVGVVHVMERLSRHQGYLPWRNAAVQFFGGIAALASGQSGGREGPAIHL
                     GAASSSLIGQIFRLPNNSLRTLVACGSAAAIASSFNTPIAGVIFAMEVVMMEYTIGSF
                     IPVIIAAVTSTLLTHYFIGDAPAFTVAPLQMQSLSEVPFVVLAGIVIGCTAAGFTALV
                     QLFARLSRWPFWSRALLAGALTGLAATMVPAVMGIGYDTVNAAMLGQLSLTALLLVAL
                     FKSISSAAAVGLGLPVGLIGPTLVIGAALGGCIGMIGNLLEPADAISEGFYVMLGMAA
                     MMAAVLQAPLAALMAVMELTLNPNLILPAMLMIVVATLVSSVAFRQR"
     gene            1359..2117
                     /locus_tag="AC16PP_09860"
     CDS             1359..2117
                     /locus_tag="AC16PP_09860"
                     /inference="COORDINATES: protein motif:HMM:NF042896.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HIR6481974.1"
                     /translation="MARDPLKGIIKGEGQVRVVAHSPRRRLLTSLTVALVCAAGAAGG
                     FYLGESKAGLDAGYIVALEKMDRANAVEIAELRSRLIDANLARSVESQAARELRDDIR
                     ILRDEVAGLQEEVTFYKSIMAPDSIERGLQIAEFQLVPGEAVNEFTYHLLLTQVEARR
                     DWIQGTVTLDVVGRRTPDPSRKDVELVLSLTEIGQTDTYPLSFRFRYFQDLTGSMALP
                     DGFLPEAVVVTARKRGARSEQLQRKFAWAMGEAE"
     gene            2179..2628
                     /locus_tag="AC16PP_09865"
     CDS             2179..2628
                     /locus_tag="AC16PP_09865"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR6481975.1"
                     /translation="MARTKVRDGVTLIAANTEVTGNVKFNGQLYVSGRVNGNILAAEG
                     CEATVVVSEEGVVSGEIRVPNVVINGTVEGDISASERIELACKARVKGSVYYALVEMQ
                     LGAQVDGQLVHEHPQTAASEDAHKAGAKRRNPLGIIKQLPKAGATAS"
     gene            2743..3087
                     /gene="erpA"
                     /locus_tag="AC16PP_09870"
     CDS             2743..3087
                     /gene="erpA"
                     /locus_tag="AC16PP_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009408529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HIR6481976.1"
                     /translation="MTGQLDIDISFTERAAAKVRALIADEENADLKLRVFITGGGCSG
                     FSYGFTFDEEVAEDDAVVEREGVSMLVDSLSYQYLLGAEVDYKEGLQGAQFVVQNPNA
                     SATCGCGNSFAI"
     gene            complement(3106..4206)
                     /locus_tag="AC16PP_09875"
     CDS             complement(3106..4206)
                     /locus_tag="AC16PP_09875"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HIR6481977.1"
                     /translation="MNGLYVGVITGTSVDGLDLALLEIDSKIRFAASETVALRDDLRD
                     ELISLGQPGQNEIDRLGAADARLGRAIGEAINEFLANEGVDPKAVSAIGSHGQTVRHR
                     PDAAAPFTLQIGDPNRIAEATGITCVADFRRRDMAAGGQGAPLVPPFHAALFGASGER
                     RIVLNIGGISNVTVLPSDPRESITGFDTGPGNCLMDGWIDHCRSMPYDREGDWAREGS
                     PSPALLAAMLADPYGLRTPPKSTGREYYNLEWLSRHPGTGHLSEADVQATLLEFTARS
                     IADAVDRWGGRAERWLVCGGGRHNRLLMERLAQLTPSPIETTDAHGVDGDALEAGAFA
                     WLAHQTMASLPGNAPGVTGASGERILGGIYRP"
     gene            complement(4226..5563)
                     /locus_tag="AC16PP_09880"
     CDS             complement(4226..5563)
                     /locus_tag="AC16PP_09880"
                     /inference="COORDINATES: protein motif:HMM:NF039309.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR6481978.1"
                     /translation="MKLASFPRPHLILVGVLSLGLCSLVVAKFADDETHDRFTTEAVL
                     LKLPAATTPDQPDAGGPAAGIQAMTPADAFSAANLEQRRAEVGRGDNLALIFQREGIP
                     AVELQRLLASEPFGPRLKRIFPGHEFTFTLDSERSLVKLSYAPGPLSALEFHRAGDTF
                     VGREVTQSPERTINYKHADIDHSLFVASQRAGLTDGLTMNLAQIFQWDIDFVLDIRSG
                     DEFHVIYEELYLGDEFIGPGNIIAAEFVNQGERYQALRYVDENGESGYYSPDGRNMRK
                     AFLRAPVEFSRISSNFNLRRKHPLADRTMPHRGIDYAAPNGTPILASGDGTVTNASRT
                     KANGNFIVLKHGEQFVTKYLHLSKFARGVRRGARVRQGQVIGYVGATGWATGPHLHYE
                     FLVNGVHKNPRTVKLPDAEPIEDTELARFNRKAVAMTSLLENYKAQTRLAYSR"
     gene            5786..7066
                     /gene="tyrS"
                     /locus_tag="AC16PP_09885"
     CDS             5786..7066
                     /gene="tyrS"
                     /locus_tag="AC16PP_09885"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR6481979.1"
                     /translation="MGIALIEELNERELIAQVSDMEALRQHLETGSRTLYCGFDPTAD
                     SLHIGNLVPLLTLRRFQLGGHRPILLLGGATGLIGDPSGRSDERNLNSGEVVAGWVER
                     IRRQVEGFVDFSGGNGAVIVNNLDWTADLPVIAFLRDVGKHFSVNTMIQRESVKSRLD
                     RDEAGISYTEFSYMLLQAMDYLELAERYGCSLQIGGSDQWGNIVSGMDLVRRKLKKEA
                     FALTLPLVTKADGTKFGKTAAGAVWIDPDRTSPYSFYQFWLNTADADVVRFLQVFTFL
                     PLERIAQLAEAAREAPERREAQQALAEEVTSLVHGDSSLESARRISRCLFQGDIRGLT
                     EADLNQLQQDGIQCTVTKEERPGLLAVLADAGLAPSRSAARKLLQGGGVSVNGVVQEH
                     PEAELDWGQALFGRYFLVRRGKKNWHLLARRKES"
     gene            7395..>8363
                     /locus_tag="AC16PP_09890"
     rRNA            7395..>8363
                     /locus_tag="AC16PP_09890"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1626 a         2447 c         2752 g         1538 t
ORIGIN      
        1 atcgctgcct gaacgccacg ctgctcacca gcgtcgccac cacgatcatc agcatggcgg
       61 gcagtattag attcgggttc agggtgagct ccatgacggc catgagcgcg gcgagcggag
      121 cctgaagcac ggcggccatc atggccgcca tgccgagcat gacgtagaac ccctcggaga
      181 tcgcatcggc cggctcgagc aggttgccga tcatgccgat gcagccgccg agagccgcgc
      241 cgatgaccaa cgtgggaccg atgaggccaa cgggcaggcc caggcccacc gccgccgcgc
      301 tgctgatcga cttgaacaga gcgacgagca gcagcgccgt aaggctcagc tgtcccagca
      361 ttgccgcgtt gaccgtgtcg tagccgattc ccatcaccgc cggcaccatg gtagcggcaa
      421 gcccggtcag ggctccggct agcagggctc ggctccagaa gggccagcgg gagagacgcg
      481 cgaaaagctg aactagcgcg gtgaatcctg ccgccgtgca gccgatgacg atcccggcca
      541 gcacgacgaa gggcacttcc gacagggact gcatctgcag tggagccaca gtgaatgcgg
      601 gcgcgtcgcc aatgaaatag tgggtcagca gggtggaagt gacggcggcg atgatgaccg
      661 ggatgaagct gccgatggtg tactccatca tgacgacctc catcgcgaag atgacccccg
      721 cgatgggcgt attaaaggag ctggcgatgg cggccgcgct gccgcaggcg accagggtgc
      781 gcaggctgtt gttgggtagc cggaagatct ggccgatcag gctcgagctt gcggcgccaa
      841 gatgaatcgc gggtccttcc cttcccccag actgaccgga cgccagggcc gcgatgccgc
      901 cgaaaaactg caccgcggcg ttgcgccacg gcaggtagcc ctggtgccgg gagagccgct
      961 ccatgacgtg gacgacgccc acccgccgct ccgccgcgga caggcggttg agcgccagcc
     1021 cgatcaggcc ggcgccggcg atgggcaggg ccaggcgctc caggatgctc agcgcttcga
     1081 aggattcact gccttcggga agcagccagc ccccgaggat gagctcgacg gccgcccgga
     1141 agagcaggat gacgcagccg gtgatcagtc cggccagaac ggccagtagc gccagctgtg
     1201 gaagcgcctc gatgtccgcc agccgattgc ggaagaactc cggggagact ttatttggta
     1261 atctcagggc cccaatctct gccgacggtg cagagctcga actggtgaat cggatcacta
     1321 gtatagtctc tggaatggcc cgaggcagat catcccgtat ggcacgtgac ccattgaaag
     1381 gcatcatcaa aggcgaggga caggtgcgcg tggtggcgca ctcgccgcgc aggcgcctgc
     1441 tcacgtcatt gaccgtcgcc ctggtctgcg cggccggtgc cgcgggtggg ttctaccttg
     1501 gcgaatccaa ggcggggctg gatgcaggct acatcgttgc cctggagaag atggaccgtg
     1561 cgaacgcggt tgagatcgcc gagctccgca gcaggctcat cgacgccaat ctcgcccgaa
     1621 gcgtggagtc acaggcggcg cgcgagctgc gcgacgacat ccggatcctc agggacgagg
     1681 tggccggtct ccaggaagag gtgactttct ataagagcat catggctccg gacagcatcg
     1741 agcggggcct gcagattgcg gaatttcagc tcgttcccgg cgaagcagtc aacgaattta
     1801 cctatcacct gctgctgacc caggtggagg cccgccggga ttggattcag ggcaccgtga
     1861 ccctcgatgt ggtgggccga agaacgccgg atccgtcacg gaaagatgta gaactggtgc
     1921 tctccctcac agaaatcggg caaaccgaca catatcctct gagtttccgg ttccggtact
     1981 tccaggacct caccgggagc atggcgttac ccgatggatt tctgccggaa gcggtggtgg
     2041 taaccgcccg gaaacgcggt gcgcgctccg agcagttgca gcgaaagttt gcctgggcca
     2101 tgggcgaagc cgaatgagcg ccgtttccta aacggacggc gagagcgcgc cagggccccc
     2161 caggaacgaa gggtcatcat ggcgagaacc aaggtccgag acggcgtaac cctgatcgca
     2221 gcgaataccg aagtcaccgg gaacgtgaag ttcaacgggc agctctacgt cagcggtcgg
     2281 gtgaacggca acattctcgc cgccgaaggc tgcgaagcca ccgtggtggt gagcgaggag
     2341 ggtgtggttt ccggcgagat ccgcgttccc aacgtggtca tcaacggcac cgtcgaaggc
     2401 gacatctctg cttccgagcg gatagaactt gcctgcaaag ccagagtcaa aggaagcgtg
     2461 tattatgcgc tggtagagat gcagctcggg gcccaggtcg acggccagct ggttcacgaa
     2521 catccccaga cggccgcgtc tgaggatgct cataaggccg gagcaaagcg caggaatccc
     2581 ctggggatca tcaagcaact gcctaaggcg ggcgcgaccg cgtcctgaag ggcacccttg
     2641 acccggtctg ggggtacttc gataattttc cgccctcgcc gcccctcggg cctcgttcat
     2701 cttgcggccg gggaaccagt gagagcgtca ccgggtaact caatgactgg acagctcgac
     2761 atcgacatca gcttcaccga gcgcgcggcc gcgaaagtgc gggcactcat cgcggatgag
     2821 gagaatgcgg atctcaaact ccgggtcttc attaccggag gtggatgcag cggcttttcc
     2881 tacggcttca cattcgacga ggaagtagcc gaagacgatg ccgtcgtcga gcgcgaaggc
     2941 gttagcatgc tcgtcgacag cctcagctat cagtacctgc tgggcgcgga ggtcgactac
     3001 aaagaaggtc tgcagggcgc gcagttcgtg gtgcagaatc ccaacgcgtc cgctacctgc
     3061 ggatgcggca actccttcgc catatgagaa ggatccgggt agccgtcacg gccggtagat
     3121 accgccgaga atgcgctctc ccgaggcacc cgtgacgccg ggcgcgttgc ccggaaggct
     3181 tgccatggtc tggtgagcca gccaggcgaa agcaccggct tccagagcat cgccgtccac
     3241 gccgtgggca tcggtcgtct cgatcgggga gggcgtcagc tgggccagcc tttccatgag
     3301 cagccggttg tggcgtccac cgccgcagac gagccatctc tccgcccgcc ccccccagcg
     3361 atccaccgcg tcggcgatgc tgcgggccgt aaactcgaga agggtggcct gaacgtcggc
     3421 ttcgctgagg tgtccggtgc cgggatgtcg gctgagccat tcgaggttgt agtactcgcg
     3481 acccgtgctt ttcggcggcg tccgcaagcc gtaggggtcc gcgagcatgg ctgcgagcag
     3541 ggccgggctg ggggagcctt cccttgccca atcgccctcg cggtcgtagg gcatcgatcg
     3601 gcaatggtcg atccagccat ccatcaggca gttgcccggt ccggtatcga aacccgtgat
     3661 gctttcgcga gggtccgacg gcagaacggt gacgttgctg atgccaccga tgttcaggac
     3721 gatgcgccgc tcgccggaag caccgaacag cgctgcgtgg aagggcggca ccagcggcgc
     3781 tccctgccca ccggcggcca tgtcccggcg cctgaaatcg gccacgcagg ttatgccggt
     3841 cgcctcggca attctgttgg ggtcgccgat ctgcagggtg aatggggcag ccgcgtcggg
     3901 tcggtggcga acggtctggc cgtgactgcc gatcgcggac accgctttgg gatcgacgcc
     3961 ctcgttcgcc agaaattcgt tgatcgcctc gccgatggcc ctgcccagcc gggcgtcagc
     4021 tgctccgaga cggtcgatct cgttctggcc gggctgaccg agactgatca actcgtctct
     4081 cagatcatct cgcaaggcga ccgtctcgct ggccgcgaac cgtatcttcg agtcgatctc
     4141 cagcagggcc aggtccaggc cgtcgacgct cgtgccggtg atgacgccta cgtaaagccc
     4201 attcacctgc ccggcccccc ggccgtcatc gggagtatgc gagtcgtgtc tgagccttgt
     4261 agttctccag cagggaagtc atggcaaccg ccttcctgtt gaaccgggca agttcggtat
     4321 cctcgatggg ctcggcatcc ggcagcttta cggtgcgggg attcttgtgg acgccgttga
     4381 cgaggaactc gtagtgcagg tgaggtcccg tggcccagcc cgttgcgccc acgtagccga
     4441 tgacctgccc ctgcctgacc cgggcccccc gccgtacgcc tcgcgcgaac ttcgacaggt
     4501 gcaggtactt cgtcacgaac tgctcgccgt gcttgagcac gatgaagttg ccgtttgcct
     4561 tggtccgcga tgcgttggtg accgtaccgt caccggaggc gagtatcggg gtgccgtttg
     4621 gggcagcgta gtcgatgccg cgatggggca tcgtgcggtc tgccagaggg tgcttgcggc
     4681 gcaggttgaa attggagctg atcctggaga actccaccgg ggcgcgcagg aatgctttgc
     4741 gcatgttgcg gccgtcgggg ctgtagtagc ccgactcgcc gttctcgtcg acgtatcgca
     4801 aagcctgata gcgttccccc tgattgacga actcggcggc gataatgttg cccgggccga
     4861 tgaactcgtc gcccaggtag agttcctcgt agattacgtg aaactcgtct cccgaccgga
     4921 tgtcgagcac gaagtcgatg tcccactgga atatctgtgc caggttcatg gtcaggccgt
     4981 cggtcaagcc cgcccgctgg ctggcgacga acaggctgtg gtcgatgtcg gcgtgtttgt
     5041 agttgatggt ccgttcggga ctctgcgtca cctcgcgccc cacgaacgtg tcgcctgcgc
     5101 gatggaactc gagggcgctc aaggggccgg gcgcgtacga tagctttacc aggctcctct
     5161 ccgaatcgag ggtgaaggtg aactcgtgtc ctgggaagat ccgcttcagg cgcgggccga
     5221 aaggctcgct cgccagcagg cgttgcagct cgacggccgg gatgccctcg cgctggaata
     5281 tcagggccag gttgtcgccg cgaccgacct cggcgcggcg ctgctccagg ttggcagcgc
     5341 tgaaggcgtc agcgggggtc atggcctgga tgccggcggc ggggccgccg gcatcaggtt
     5401 gatcgggtgt ggtcgcagca gggagcttca gcaggacggc ttcagtggtg aagcggtcgt
     5461 gggtttcgtc gtcggcaaat ttcgccacga ccagcgagca caggcccagg ctcaacacgc
     5521 cgaccagaat caggtggggt ctcgggaacg aggcgagctt cactgcgaca ggacgccctt
     5581 ttgaaatctt tatcgaaaac agttggttag accctgttct tcagatctgc caaccagctt
     5641 ggctagtata ttgatttcgt tcccgttgaa caccctgaag gtaggggtca attgggccag
     5701 gttgtaaccg tgacgctact tccgctgccg tgcatttggt aaggtgcgcg tcccgagttt
     5761 ggccactagc gacagggacc ctggaatggg catcgcgctg atcgaagaac tgaatgagcg
     5821 ggaattgatc gcgcaggttt ccgatatgga ggcacttcgc cagcatctgg agaccggctc
     5881 gcgcacgctc tactgcggct tcgatcccac ggcggacagc ctgcacatcg gcaatctggt
     5941 ccctctgctc actttgcgcc gtttccagct cggcgggcac cgaccgattc tcctgcttgg
     6001 tggtgcgacg ggtctcatcg gcgacccttc gggccgcagt gatgagcgga acctcaacag
     6061 cggggaggtc gtggcgggat gggtcgaacg gatccgacgg caggtggagg gtttcgttga
     6121 tttctcaggc ggcaacggtg ccgtgatcgt caacaacctg gactggacgg ccgacctgcc
     6181 ggtgatcgcc tttcttcggg acgtgggcaa gcacttctcg gtcaacacca tgattcagcg
     6241 ggaaagcgtc aaatcccgtc tggaccggga tgaggctgga atttcctaca cggaattcag
     6301 ctacatgctt ctccaggcca tggattacct ggagctggcc gagcgttacg gctgctcgct
     6361 gcagatcggt ggaagcgacc agtggggcaa catcgtctcg ggcatggatc tcgtgcggcg
     6421 caagctcaag aaagaggcct ttgccctgac cctcccactc gtcaccaaag ccgatggtac
     6481 caagttcggc aagacggcgg ccggcgcggt ttggatcgat cccgaccgca cctcccccta
     6541 cagcttctat cagttctggc taaacaccgc cgacgcggac gtggtccgtt tccttcaggt
     6601 cttcactttt ctgcccctgg aacggatcgc gcagctcgcc gaagcggcgc gggaggcacc
     6661 cgagcggcgc gaggcgcagc aggcgctggc agaggaagtg acttcgctcg tgcatggcga
     6721 cagctctttg gagtcggctc ggcgcatctc gcgctgtctg ttccagggcg atatccgggg
     6781 gctgacggag gccgacctga atcagcttca gcaggacggg attcagtgca cggtcactaa
     6841 agaggaaagg cccggtcttc tggccgttct ggccgatgcc gggctggcac cctcccggtc
     6901 ggccgcacgc aagctgctcc agggcggcgg ggtcagcgta aacggcgtcg ttcaggagca
     6961 tcccgaggcg gaactggact ggggtcaggc gctcttcggg cggtattttc tcgtccgacg
     7021 gggcaagaaa aactggcatc tcctggcccg ccggaaagaa tcttaaaatg ccttgctaag
     7081 gccctacccc tgcgtataat acgcctccct tgcccgggcc cctcggccgt cagggccgct
     7141 cattaaaatt tgaaatactg aacaagccat tcgtgtgggt gcttgtaatt cattgttgcc
     7201 agagggtttt tcaggcccga tggcgagtga aaatcgagca gctccggtag gccaaaggga
     7261 cgggtagact ttgtcgagcc ggcctagaag gtttctgggg catagcacgt aaattcattg
     7321 agcaaagatt gatttgtggt ctcggtggcg tccgccccta accgggcagg ctccaaagaa
     7381 accacgctct ttataaactg aagagtttga tcatggctca gattgaacgt tggcggcacg
     7441 cctaatacat gcaagtcgaa cgagaaagcg ccttcgggtg tgagtaaagt ggcggacggg
     7501 tgagtaacgc gtaggaattt gcctagcagt gggggacaac tcggggaaac tcgagctaat
     7561 accgcataat atctacggat taaagtgggg gatcttcgga cctcacgctg ttagaggagc
     7621 ctgcgttgga ttagcttgtt ggtggggtaa cggctcacca aggcgacgat ccatagctgg
     7681 tctgagagga cgatcagcca cactggaact gagacacggt ccagactcct acgggaggca
     7741 gcagtaggga atcttgggca atgggcgaaa gcctgaccca gcgatgccgc gtgtgtgaag
     7801 aaggccttcg ggttgtaaag cactttcagc agcgaagaaa agtcgggagc taataactcc
     7861 caggctctga cgttaactgc agaagaagta ccggctaact ccgtgccagc agccgcggta
     7921 atacggaggg tacgaacgtt aatcggaatt actgggcgta aagcgcgcgt aggcggttcg
     7981 gcaagtggga tgtgaaagcc ctgggcttaa cctgggaact gcattccaaa ctgtcgagct
     8041 agagtatggt agagggaggt agaatttcct gtgtagcggt gaaatgcgta gatataggaa
     8101 ggaataccgg tggcgaaggc ggcctcctgg accaatactg acgctgaggt gcgaaagcgt
     8161 ggggagcaaa caggattaga taccctggta gtccacgccg taaacgatga gaactagccg
     8221 ttggggtcct tgagatctta gtggcgcagc taacgcgata agttctccgc ctggggagta
     8281 cggccgcaag gttaaaactc aaatgaattg acgggggccc gcacaagcgg tggagcatgt
     8341 tgtttaattc gatgcaacgc gaa
//
LOCUS       DBNHKV010000313         1518 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1814956, whole genome shotgun sequence.
ACCESSION   DBNHKV010000313 DBNHKV010000000
VERSION     DBNHKV010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1814956"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="AC16PP_09895"
     CDS             <1..314
                     /locus_tag="AC16PP_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481980.1"
                     /translation="LDPLWSWPERELAGSLLPREQAIGIWEQASGITVDRDAFRWWQV
                     FASYKGLAIWISSTEDFVNGASKEPILAVAGWLMSDRQNRILVDRLNPRSDHAYAEPI
                     V"
     gene            311..724
                     /locus_tag="AC16PP_09900"
     CDS             311..724
                     /locus_tag="AC16PP_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481981.1"
                     /translation="MIPNPSKALVDLAMKLSTGVLPEIGSPYAAANAGMTAVLLIMLS
                     QDSERAIANRMNDIDELKALFADAAAFPDRVGLASFVDAEPDSLLLADVDRLHADGMR
                     LLIDLHAWAESHDAGLDQEIWAFLGRHADRNRFDV"
     gene            825..900
                     /locus_tag="AC16PP_09905"
     tRNA            825..900
                     /locus_tag="AC16PP_09905"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:858..860,aa:Lys,seq:ttt)
     gene            complement(908..>1518)
                     /gene="rfaP"
                     /locus_tag="AC16PP_09910"
     CDS             complement(908..>1518)
                     /gene="rfaP"
                     /locus_tag="AC16PP_09910"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011703.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HIR6481982.1"
                     /translation="YRATRALSAAGVSTLEIAGFGLRGRNPARRRSFLITDELAPVKS
                     LEAYVLRWPEEPHAPGLKRRLIEEVAAISRAMHGAGINHRDYYLCHLLMLHPELFGPQ
                     APDPRIFVVDLHRAEIRNAVPYRWLVKDLGSLYFSAYEIALTRGDRLRFLAAYFQMPW
                     RDVLARHSGLIADIAKRADRLYRKGRRKGILPRQVAGLAPTR"
BASE COUNT          273 a          511 c          472 g          262 t
ORIGIN      
        1 ccctcgatcc cttgtggtca tggcccgagc gcgagcttgc cggctccctg ctgccgcggg
       61 agcaggccat cggtatttgg gaacaagcca gcggcatcac cgtggacaga gacgccttcc
      121 gctggtggca ggtattcgcc tcctacaagg ggctggcgat ctggatctcc tccaccgagg
      181 acttcgtcaa cggcgcgtcc aaggagccca tactggcagt ggccggctgg ctgatgagcg
      241 accgtcagaa ccggattctg gtggaccgct tgaacccccg ctccgaccac gcctacgcgg
      301 agcccatcgt atgattccca acccctcgaa ggctctcgtc gacctggcca tgaagctttc
      361 caccggcgtg cttccggaaa tcggctcacc ttacgcggca gcgaatgcgg gcatgacggc
      421 cgtcctgctg atcatgctct cacaggacag cgaacgggcc atcgcgaacc gcatgaacga
      481 catcgacgag ctcaaagctc ttttcgcgga cgccgcggcg tttccggatc gggtcgggct
      541 cgcgtccttc gtcgatgccg agcccgactc gctcctgctg gcagacgtgg accggctgca
      601 cgcagacggt atgaggctcc tcatcgacct gcacgcctgg gcggaatccc acgacgccgg
      661 gctcgatcag gagatctggg cattcctggg acgccacgcc gatcggaacc gcttcgacgt
      721 ctgaacgggc gccgctccca tggaagcgac cccaccggca agggttgaga tgggccctta
      781 cgctgactac aattcgcgct cttttttgcc gccatccggc aagagggtcg ttagctcagt
      841 tggtagagca ccgggctttt aacccgttgg tcctgggttc gagccccaga cgacccacca
      901 cctccactca tcgcgtcggc gccagcccgg caacctggcg tggcaggatc ccctttcttc
      961 gccccttccg gtacaagcga tcggcccgct ttgcgatgtc ggcgatcaga ccggaatgcc
     1021 gagcgagaac gtcgcgccac ggcatctgaa agtaggctgc cagaaatcgc aggcggtccc
     1081 cccgggtcag ggcgatctcg taagcggaga aatagaggct gcccagatcc ttgaccagcc
     1141 accggtacgg cacggcgttc cggatctcgg cgcgatgcag gtcaaccacg aagatgcggg
     1201 ggtccggggc ctgcggccca aagagctcgg gatgcagcat cagaaggtga cacaggtagt
     1261 agtccctgtg attgataccc gccccgtgca tggcgcggga gatggcggcc acctcctcga
     1321 tcaaccggcg tttcagccca ggcgcgtgcg gctcctccgg ccagcgcagc acgtaggctt
     1381 caaggctctt taccggtgca agctcgtcgg tgatgagaaa cgaccggcgc ctcgcggggt
     1441 tacgcccacg cagcccgaag ccggcgattt ccagggtgct caccccggca gccgagagcg
     1501 ctcgagtggc acgatact
//
LOCUS       DBNHKV010000314         2559 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_469570, whole genome shotgun sequence.
ACCESSION   DBNHKV010000314 DBNHKV010000000
VERSION     DBNHKV010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_469570"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="AC16PP_09915"
     CDS             complement(<1..476)
                     /locus_tag="AC16PP_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481983.1"
                     /translation="MESDKDDQPAAKKTTGKPAKKRSKPAPTGAFGSTVWAPLLGTLG
                     VVCAFALLWLQLIESGNANHRELQTAVHRDFLVAHFNDRIANLRNQVRSIARTRTVTE
                     AATSEDPATIADAGSALSDLSAFALRVDIFRPGQAVLDKEAEVPISYAALDLIRRA"
     gene            complement(548..1825)
                     /gene="coaBC"
                     /locus_tag="AC16PP_09920"
     CDS             complement(548..1825)
                     /gene="coaBC"
                     /locus_tag="AC16PP_09920"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017526818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR6481984.1"
                     /translation="MTQPDNQAPANLGGRKILLGVSGGIAAYKAPILVRLLRQAGADV
                     QVVLTRSATRFVTATTLQAVSGRPIRDDLWDAAAEAAMGHIELARWADAVLIAPATAE
                     MLSRLATGRADDLLTTLVLATRAPIFVAPAMNNAMWDHPATQRNASTLTDRGVNVLGP
                     DSGEQACGEFGPGRMREPADLVADLCASFEPKDHLLAGKNVLVTAGPTREAIDPVRYV
                     TNHSSGKQGYAMAAAARAAGAQVTLVSGPVHLPTPIGVRRIDVTSAAEMHQAVLAHAS
                     TCDVFIGVAAVADYRPDRAHDQKIKKTDTGSGESGLSLRLVENPDIIAGVSSLERRPF
                     VVGFAAETHDALSHARDKRKRKGMDLIVVNDVSDSSIGFNSDQNAATVIWEDGELSIP
                     RQGKDLVARAVIDLIARQIRDPLAGANPERIAK"
     gene            1906..>2559
                     /gene="radC"
                     /locus_tag="AC16PP_09925"
     CDS             1906..>2559
                     /gene="radC"
                     /locus_tag="AC16PP_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIR6481985.1"
                     /translation="MATVKWPDSDQPRNRLLQAGPDRLTDAELLAVCLRTGHGPQDPV
                     TYARSLLRRFGHIGKLLDASPDRLLGMPGMGPAKAAALKASLGVAERYLRAGLGGGRL
                     MGDSRAVTDFLKHQLGSLQREVFACLFLDARHRFIAFEKMFLGSLDRASVHPREVLKR
                     TLELNAAAVIFAHNHPSGIAEPSASDVQITEELKALLERLEVCVLDHVVIGRGCQVSF
                     "
BASE COUNT          392 a          790 c          888 g          489 t
ORIGIN      
        1 tcggctcttc tgatcagatc gagggcggcg taggaaattg gcacctcggc ttctttgtcc
       61 aggacggcct ggcctggccg gaagatgtcc acgcgcaggg caaaggcgga caggtcgctc
      121 agcgcgctgc ctgcatcggc gatggtggcc gggtcttcgc tggtggctgc ctcggtgacg
      181 gtgcgcgttc tcgcgatgga gcgcacctga ttgcgcagat tggctatgcg gtcgttgaag
      241 tgggcgacga ggaagtcgcg gtgcacggcc gtctgcaact ctcgatgatt ggcgttgccg
      301 gattcgatca gctgcagcca aagcagcgcg aaggcgcaga ccacgccgag agtgccgaga
      361 agcggtgccc agaccgtcga gccgaacgcg ccggtgggag cgggctttga tctcttctta
      421 gcgggctttc cggtggtctt cttggcggcg ggctggtcgt ctttgtcgct ttccaagggt
      481 caggatccag gcgtccgggg cggggcaatg gtaatgcgga tatgccaggg caatccagcg
      541 ccgcgcgcta ttttgcgatg cgttcggggt ttgcgcccgc taacgggtcg cgtatctgcc
      601 gggcaatcag atcgatcacc gcgcgggcaa cgaggtcctt gccctgccgc ggaatcgaca
      661 gctctccgtc ttcccagatg accgtggcag cattctggtc gctgttgaaa ccaatgctcg
      721 aatccgacac gtcgttgacg acgatgaggt ccatgccctt gcgcttgcgc ttgtcgcgcg
      781 cgtggctcaa cgcatcgtgg gtctcggcgg cgaacccgac gacgaaagga cgtcgctcca
      841 ggctggatac gccggcgatg atgtccgggt tctcgaccag tctcaaggag aggcccgatt
      901 cgccggatcc tgtgtcggtt ttctttatct tctggtcatg agcgcggtcg ggacgataat
      961 cggctaccgc ggccacgccg atgaacacgt cgcaagtgct ggcgtgtgcc aggacggcct
     1021 ggtgcatctc tgcggcgctc gtgacgtcga tgcgccgcac gccgatgggt gtggggaggt
     1081 gcacgggtcc ggagaccagg gtcacctggg caccggcggc ccgggcggcg gcggccatgg
     1141 cgtacccctg ctttccggag ctgtggttgg ttacgtagcg aaccgggtcg atggcctcgc
     1201 gggtgggccc ggctgtaacg aggacgttct tgccggccag cagatggtcc ttaggctcga
     1261 aagacgcaca caggtcggca accagatccg cgggctcgcg catgcggccc ggaccgaatt
     1321 ccccgcacgc ctgctcgccg ctgtcgggcc ccagcacgtt cacgccccgg tctgtcaggg
     1381 tgctcgcatt gcgctgggtg gccgggtgat cccacatggc gttgttcatc gcgggagcga
     1441 cgaagatcgg cgctctagtg gccagaacca gggtcgtcag caggtcgtcg gcgcggccgg
     1501 tggcaagccg gctgagcatc tcggcagttg ccggggcgat gagcacggcg tcggcccagc
     1561 gggcgagctc gatgtggccc atggcggctt cggccgcggc gtcccagagg tcgtccctga
     1621 tgggcctgcc ggagacggcc tgcagggtag tcgcggtgac gaaccgcgtc gcgctgcggg
     1681 tcagaaccac ctggacgtct gccccggcct gtcgcagaag gcgcacaaga attggggcct
     1741 tgtacgccgc gatgcctccg ctgaccccga gtaggatctt tctgcccccg agatttgccg
     1801 gcgcctggtt gtctggctgc gtcatcgttt agctgttggg cctggctgga aactgattga
     1861 gcctaaagat accaccgcca ccgattcgcc gcaggaggtt ttcccatggc cacggtcaaa
     1921 tggcctgaca gtgaccagcc ccgaaatcgt cttctccagg ccggacccga tcgtctgacg
     1981 gacgccgagc tgctcgccgt gtgcctaaga accgggcacg gcccccagga cccggttacg
     2041 tatgcgcgct cgctgcttcg gcgttttggg cacatcggca agctgctgga cgcctctccc
     2101 gatcgcctgc tcggaatgcc tgggatgggg ccggcgaaag ccgccgcgct gaaagccagc
     2161 ctgggggtcg ccgagcggta cctgcgtgcc ggcctcggcg gcggccgtct gatgggtgac
     2221 tctcgggccg tgaccgactt tctcaagcat cagctcggca gtttgcagcg ggaagtgttc
     2281 gcctgcctgt tcctggacgc caggcatcgg ttcatcgcct tcgagaagat gtttctcgga
     2341 tcgctggatc gggccagcgt gcatccaaga gaggtgctta aacggactct ggagctcaac
     2401 gcggcggcgg tgattttcgc tcacaaccat ccctccggca tcgccgagcc gagcgccagc
     2461 gacgtccaga tcaccgaaga gctcaaagcg ctgctggagc ggctggaggt gtgcgtgctc
     2521 gatcacgtcg tgatcgggcg cggttgccag gtctccttc
//
LOCUS       DBNHKV010000315         1923 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1746396, whole genome shotgun sequence.
ACCESSION   DBNHKV010000315 DBNHKV010000000
VERSION     DBNHKV010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1746396"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="AC16PP_09930"
     CDS             <1..368
                     /locus_tag="AC16PP_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6481986.1"
                     /translation="TLARSGLDRAAVKRAYDAPFAEGGRSKAGARRFPWCLPFGEPEA
                     GNAADQARCFEALKSWAKPAHFIFGERDPIFTTGWARQWSALLPNATLDLIPDASHFV
                     QEEAGEEIVAAFLERTRST"
     gene            432..818
                     /locus_tag="AC16PP_09935"
     CDS             432..818
                     /locus_tag="AC16PP_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6481987.1"
                     /translation="MIDINDRMAILDLCASYNYLFDTCAAKEWAATFTADGVFEGPAG
                     RAEGPEDLAEFCRNTNAQYPGAMHFTDNHLFEQNGDTVRHKCFLSMQVPSPEGTQTLL
                     LTYEDELRRVDGEWRFASRLCQPLSA"
     gene            complement(830..>1923)
                     /locus_tag="AC16PP_09940"
     CDS             complement(830..>1923)
                     /locus_tag="AC16PP_09940"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6481988.1"
                     /translation="PSSYDVNSPGGFPVLVYLHGWGGSFRSLPASAARHAKNNGYILV
                     TPTGYGDGGRNSWNGYRSARLPVCALGDGYLASDCDAGPPELDSANGPSTCVDLDNAG
                     HDYCYDSCRTLYGAGAEPGNCPVWDGSQGDNTWGAEYNGDYTCHWTTCRDSVGQIAAL
                     LDEIEALYCVDRSMIWVTGCSNGGMMVYELAKDARTRTRFAGFMPQVGAPHPGFRQHD
                     PASELVDRPRHLLGFWGETDRTVPGRANFPGTSPQVALDTSFNGWLYVPASDIGYEWA
                     SMHDAAPLPVEEADAYARAYSNSLDCQVWQAQDEGETPGHASEVATCFFQGGHSCPGF
                     GGMEAMMWDFAGRHAKTDADPAACSTSSP"
BASE COUNT          332 a          664 c          632 g          295 t
ORIGIN      
        1 gcacgctggc ccgcagcggg ctggaccgag cggccgtgaa gcgcgcttac gacgcgccat
       61 tcgctgaggg cggccgctcc aaggcaggcg cgcgccggtt cccatggtgc cttccattcg
      121 gcgagccgga ggccggcaac gccgctgatc aggcgcgctg cttcgaagcg ctaaaaagct
      181 gggccaagcc cgcccatttc attttcggcg agagggaccc gatattcacc acgggctggg
      241 ccaggcaatg gtcggcgctg ctgcccaacg ccaccctcga tctgatcccc gacgccagcc
      301 acttcgtgca ggaggaggcc ggcgaggaga tcgtcgccgc atttctggag cgcacgcgct
      361 cgacgtgatc ggcaccgcgc gtctagcatg gcctggcctg acgaacccag acgagccttc
      421 agggagatct catgatcgac atcaacgacc gcatggccat tctcgacctt tgtgcgagct
      481 acaactacct cttcgacacc tgcgccgcga aggagtgggc ggccacgttc accgccgacg
      541 gcgtcttcga aggcccggcc ggacgggcgg agggtccgga ggacctcgcc gagttctgcc
      601 ggaacaccaa cgcgcagtac ccgggcgcca tgcacttcac cgacaaccac ctcttcgagc
      661 agaacggcga cacggtcagg cacaagtgct tcctgagcat gcaggttccc tcccccgagg
      721 gcacccagac cctactgctc acctacgagg acgagctgag acgcgtcgac ggcgagtggc
      781 gcttcgcctc acgcctgtgc cagccgctga gcgcatagcc tgacgaccac taagggctgg
      841 aagtggagca cgccgccgga tccgcgtccg tcttggcgtg gcggccggcg aagtcccaca
      901 tcatcgcctc catgccgcca aagcccggac aggagtgccc cccctggaag aagcaggtcg
      961 cgacctcgga ggcatgacca ggcgtttcac cctcgtcctg ggcctgccag acctggcaat
     1021 cgagactgtt cgagtaggcg cgcgcgtagg cgtcggcttc ttcgacaggc agcggcgccg
     1081 catcgtgcat cgatgcccac tcgtacccga tgtcgctcgc cggcacgtaa agccagccgt
     1141 tgaagctcgt gtcgagagca acctggggcg acgtcccagg aaagttggca cgaccgggca
     1201 ccgtccggtc cgtctctccc cagaacccca gcaggtgacg gggccggtcc acgagctcgc
     1261 ttgcggggtc gtgctgccgg aaacccggat ggggcgcgcc cacctgaggc atgaagccgg
     1321 caaaccgggt gcgcgttcgc gcatccttgg cgagctcgta gaccatcatc ccgccgttgg
     1381 agcagccggt cacccagatc atgctgcggt ccacgcagta aagcgcctcg atctcgtcga
     1441 gcagcgccgc gatctggccc accgagtcgc gacaggtggt ccagtggcag gtgtagtcgc
     1501 cgttgtactc agcgccccag gtattgtcac cctgactgcc gtcccagacg gggcagttcc
     1561 ccggctcggc tcccgctccg tagagggtcc tgcagctgtc gtagcagtag tcgtggccgg
     1621 cgttgtcgag atccacgcag gtggacggcc cattcgccga gtccagctcg ggcggcccgg
     1681 catcgcagtc ggaggcgagg tatccgtccc cgagcgcgca cacgggcagc cgggcgctgc
     1741 gatagccgtt ccaggaattc cggccgccgt cgccgtagcc ggtgggggtc acgaggatgt
     1801 aaccgttgtt tttggcatga cgggcggccg acgccggcag ggatcgaaag ctcccacccc
     1861 agccgtgcag gtagacgagc accgggaacc cgccggggga gttgacgtcg tagctcgacg
     1921 gca
//
LOCUS       DBNHKV010000316         7407 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1956351, whole genome shotgun sequence.
ACCESSION   DBNHKV010000316 DBNHKV010000000
VERSION     DBNHKV010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7407)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7407
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1956351"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2032
                     /locus_tag="AC16PP_09945"
     CDS             <1..2032
                     /locus_tag="AC16PP_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481989.1"
                     /translation="ARQLPLEESASELGAAAGRYLSLFLEFRDGEVAPQPAPVPDDVD
                     LRADDVKGLAYFFDADHVGICELPSGARLIESDALDHAMAVVILVRFGRQPEADNLAR
                     DWIAGAEHDVAELRAAEIAVTVAGYIRQLGYPATAHVRRATTVDLDRLAVLSGVCVRR
                     DGAVCSPYFGDGFAAAAVTTDYALAVDAPLAPDQGKARGRRYHRGQNGAVSGRERRRR
                     DRRASHLSRYPMEQVRRVERPTTLIIDDEVPRVPKRANFFTRAAFGDLGEKSQREVRR
                     FAYKHPSTAGTMQLIRSMVSLQDGEIAGSRWASLDSKANAAAIKSLGYHLGADLVGIC
                     EIPPYAWYSHQADGTLIEPYHRFAVVMLIDQGFDTMEGASGDDWISGAQSMRGYVRGA
                     EIAGVMAEFLRSVGVSARPQTNADSDVLHIPLILWAGLGELSRIGELVLNPFVGPRFK
                     SVVLTTDLPLEVDAPIDFGLQYFCNHCLKCARECPCNAIPTGSKVMFNGYEIWKPDVE
                     RCTRYRITNQKGAACGRCMKTCPLNKVVGADGALAHRAGNWLAIHLPWLRPLLVPIAT
                     RLDDRTGYGARNPVKKWWFDLEIVDGVCVHAEANQRDLDVGRSAASRRDTVGYHPADT
                     MPAPDAQGPVPVNHREAVGRSALLETPADARRRKRKGEPGPSHYVPLVDVTD"
     gene            2133..2588
                     /locus_tag="AC16PP_09950"
     CDS             2133..2588
                     /locus_tag="AC16PP_09950"
                     /inference="COORDINATES: protein motif:HMM:NF047172.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6481990.1"
                     /translation="MNDFDRSLPMLLYRALDLIMPRFRAIFGGFGLTEQQWRVLRVLW
                     QRDGRALLELADATLISAPSMVGVVDRLARSGLVERRRSQSDRRVVRVFLTGRGRDLE
                     EQVTPLVDAAYADLRGSVGEGDWEVLLGALARLNAGLADLDRIDEVVNR"
     gene            2613..4064
                     /gene="hpaB"
                     /locus_tag="AC16PP_09955"
     CDS             2613..4064
                     /gene="hpaB"
                     /locus_tag="AC16PP_09955"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HIR6481991.1"
                     /translation="MGIRTGAQYLEGLRDGREVWMHGRRIEDVTTEPGLCRGAHTLAD
                     FLDRVHEPAHREKLSYAEAGSDERFSLSFLKPGSKEDVERRGAAFYEWAKWSGGMFGR
                     TPDYKNASVMAFAAASEFLAGEGGRPEFAENMRRYYEKARVEDLVLTHTLVNPQFNAA
                     LAAAGRSSPEVALQIVGENDAGVVVNGARLLATLGPHADEIEVFPSTLLKASPENVPF
                     AIAFTIPIDTRGLRLICRDTYDAGKSHFDAPLASRFEEMDAVVIFDNVLVPWERVFMY
                     QAPLLCNRAFAETNAVVHMMHQVACGKLAKAEFIVGLLCAMAKASGKDKDANVKGQIA
                     EAMWIVETVRAFLFSAEQQAEPDRWGLWVPKRLPLDTSRNLFPRLYPKLVETVHMLGS
                     SSLMATPCEADFENAIAGDVEQYFQLTNLESRDRVALFRLAHDVAVSGFGNRQVLYER
                     FFFGPQPVMASIYYDLYDKDSVMDRVTEMLKGC"
     gene            4187..4618
                     /gene="hpaR"
                     /locus_tag="AC16PP_09960"
     CDS             4187..4618
                     /gene="hpaR"
                     /locus_tag="AC16PP_09960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HIR6481992.1"
                     /translation="MLLLRARETSMAYFRPSLQERGLTEQQWRVLRALQESGELSASG
                     LSHACVILAPSMTRILRRLAEEGLVSSKRSRRDGRELTVRLTRAGERLIDEIAPISER
                     QYALIRERLQPEKLEQLHSLLHDFVELDGEHLKGAASGETQ"
     gene            4615..6138
                     /gene="hpaE"
                     /locus_tag="AC16PP_09965"
     CDS             4615..6138
                     /gene="hpaE"
                     /locus_tag="AC16PP_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010324201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HIR6481993.1"
                     /translation="MSQLLHENLAKLDRHLARFRNRTLGHLIDGEEAEGVSGETFTNT
                     TPVDDSPVNEVARGAEEDVTRATRAAVRAFGEWRDMPGARRRGLLHGIADAIEARAEE
                     IALVESYDTGQPLRFMSSAALRGAENFRFYADRAPEARNGLSTPADQHVNYSVRQPIG
                     PVGVITPWNTPFMLSTWKIAPALAAGCTVVHKPAEWSPLSASILAEICRDAGLPAGVL
                     NTVHGLGEEAGVALTEQPDIRAIAFVGESQTGRAIMSQGASTLKRVHFELGGKNPVIV
                     FDDADLERALDACTFMIYSLNGERCTSSSRLLVQATIYDEFCARVAERAGQIRVGHPL
                     DPATEVGPLIHPRHLEKVLGYCALAQTEGATVAVGGGPPGDAAAGNYVAPTLFTHARN
                     DMRIAQEEIFGPVLTAIPFDDEAEAVELANQVEFGLAGYVWTGDVGRSHRLARDLDAG
                     MIWVNSENNRHLPTPFGGMKASGIGRDGGDYSFDFYMETKNVCISLGKHRVPSLGKG"
     gene            6146..6460
                     /locus_tag="AC16PP_09970"
     CDS             6146..6460
                     /locus_tag="AC16PP_09970"
                     /inference="COORDINATES: protein motif:HMM:NF019366.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481994.1"
                     /translation="MSLYYVQKLLYQLNWDPHARRRYEEDFDELLAEYDLDAEELHAI
                     REPDIGLLYVMGVNGQILMHYAALRGYAWDEYIKAMRDGLAEHGDVRAGLYVTTDGRG
                     AV"
     gene            6460..7278
                     /locus_tag="AC16PP_09975"
     CDS             6460..7278
                     /locus_tag="AC16PP_09975"
                     /inference="COORDINATES: protein motif:HMM:NF014899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DODA-type extradiol aromatic ring-opening family
                     dioxygenase"
                     /protein_id="HIR6481995.1"
                     /translation="MGVVFAGVVSHAPGITGRAERADPALRDALYENFYRLGRELKES
                     RPDALIVIAAEHFANFFMDNMPAYCMGMADYYEGPIEDEQWLGISRVRIPGNAALAGS
                     LIESMLAEFDLAYAQEWKFDHGVMVPLHFLTPEYDIPVIPVNVNCQGPPLTPLARAYD
                     FGRALRRACDARPERIAIIGTGGISHWPATPDSGKINEAWDRAFLESWLANDRAALTS
                     FTDEETYLEAGQGGFEIRTFIAVSGATQGCAAELYFYEPIPIFAVGCTVARVHV"
BASE COUNT         1284 a         2306 c         2491 g         1326 t
ORIGIN      
        1 ggcaaggcag ctgccgctgg aagagtcggc gtctgagctt ggcgcggccg ccgggcgcta
       61 cctctcgctc ttcctggagt ttcgcgacgg cgaggtggct cctcagcccg cgcccgtccc
      121 cgacgacgtc gacctccgtg cggacgacgt caaaggtctg gcctacttct tcgatgcgga
      181 tcacgtgggt atctgcgagc tcccatcggg cgcacgactg attgaatctg atgccctgga
      241 tcacgccatg gccgtcgtca tcctcgttcg cttcgggcgc cagccggaag ccgacaacct
      301 cgcccgggac tggattgcgg gcgccgagca cgatgtggcg gagctcagag cggcggagat
      361 cgccgtcacg gtggccggct acatccggca gctgggctac ccggccaccg ctcacgtccg
      421 gcgggccacc accgtggacc tggaccggct ggccgtcctg tccggcgtct gcgtgcgcag
      481 ggacggtgcc gtctgctccc cgtactttgg tgacggcttc gctgcagccg cggtgaccac
      541 ggattacgcc ctggcggtgg atgcccctct ggccccggac cagggcaagg cccggggccg
      601 gcgctaccac cggggtcaaa acggcgcggt ctccggcaga gaacgccgtc gccgggaccg
      661 gcgtgcgtct cacttgagcc gctatcccat ggagcaggtc cgtcgggtcg aacggccgac
      721 cacgctgatc atcgacgatg aggttcccag ggttccgaag cgggcgaact tttttacgcg
      781 ggcggctttt ggcgaccttg gcgagaagtc ccagcgcgag gtccgccggt ttgcctacaa
      841 gcacccttcc acggcgggga ccatgcagct catcaggagc atggtgtcgt tgcaggacgg
      901 ggagatcgcc ggttcccgtt gggcctcgct ggactcgaaa gccaacgctg cggccataaa
      961 gtcgctcggc taccacctcg gggcggatct cgtgggcatc tgcgagattc cgccgtatgc
     1021 ctggtactcc catcaggcag acggaacgct catcgagccg tatcaccgct ttgctgtggt
     1081 gatgctcatc gatcagggct tcgacaccat ggagggcgcc agcggggacg actggatttc
     1141 cggtgcgcag tccatgcgag gctacgtgcg tggtgcggaa atcgctggcg tgatggccga
     1201 gtttctgcgc tccgtgggag tctcggcgcg accgcagacc aacgcggaca gcgacgttct
     1261 gcacataccg ctgattctgt gggcgggcct tggcgagctc agccgaatcg gcgagctggt
     1321 gctgaacccg ttcgtcggtc cccgtttcaa gtccgtggtg ctgaccacgg atctgcccct
     1381 ggaggtggac gcgcccatag acttcgggct gcagtatttc tgcaatcatt gcctgaagtg
     1441 cgcgcgggaa tgtccctgca acgcgattcc tacgggctcc aaggtcatgt tcaacgggta
     1501 cgagatctgg aagccggacg tggagcggtg cacgcgctac cgcattacca accagaaagg
     1561 ggcggcctgc ggaaggtgca tgaagacctg cccgctcaac aaggtggtgg gcgccgatgg
     1621 cgctcttgcg caccgagccg gcaactggct ggcaattcat cttccctggc tgcggccgct
     1681 cctggttccc atcgccacgc ggctggatga ccggaccggc tacggggccc gtaacccggt
     1741 gaagaagtgg tggttcgatc tggagatcgt cgacggcgta tgcgtccatg cagaagcgaa
     1801 ccagcgcgac ctcgacgtcg gtcggtcggc ggcttcccgg cgcgataccg tgggctacca
     1861 tccggcggat acgatgccgg ctcccgatgc tcagggtccc gttcccgtca accaccggga
     1921 agccgttggc cggagcgcct tgctggaaac ccctgccgat gcgcgtcgcc ggaaacgcaa
     1981 aggcgagcct ggcccctctc attacgttcc gctcgtcgac gtcaccgatt gaccccaccg
     2041 cttttcggcc cccttgcagc cgatgattac ccggttgacg cgtctcgaga tttaactatc
     2101 attttaatta ttaatacatg aactaaaagc gcgtgaacga cttcgaccga tccctgccca
     2161 tgctgcttta ccgggccctt gacctgatca tgcccagatt tcgcgccata ttcggcgggt
     2221 tcgggctcac tgagcagcag tggcgcgtgc tgcgggtgct gtggcagagg gatggtcgtg
     2281 cgctgctcga acttgccgac gccaccctga tctcggcgcc cagcatggtc ggggtggtgg
     2341 atcgcctggc gcgcagcggt ctggtggaac gacgccggtc gcagagtgat cggcgggtcg
     2401 tgcgggtgtt tctcaccggg cgcggcaggg acttagagga acaggtcacg cccctggtgg
     2461 acgcggccta cgcggacctg cgcgggagcg tgggggaggg tgactgggag gttctgctcg
     2521 gcgcgctggc ccggctgaat gcagggcttg ctgacctgga ccggatcgac gaggtggtca
     2581 atcgctgaac cggcgaagcg aggagattta cgatgggaat cagaacgggt gcgcaatacc
     2641 tcgaagggct cagagacggc cgtgaggtct ggatgcatgg caggcggata gaggacgtga
     2701 cgaccgagcc ggggctctgc cgcggtgccc acacgctggc ggattttctg gatcgggttc
     2761 acgagcctgc ccatcgcgaa aaactctcct atgccgaggc gggtagcgac gagcgattca
     2821 gcctgtcctt cctcaagccc gggtccaagg aggacgtgga gcgccgaggt gccgcgttct
     2881 acgaatgggc gaagtggtcg ggcggcatgt tcggacgcac gcccgactac aagaacgcct
     2941 cggtgatggc gtttgccgcg gcgtcggaat ttctcgccgg ggagggcggg cgaccggagt
     3001 tcgcggagaa catgcgtcgc tactacgaaa aggcccgtgt ggaggacctg gtgctcaccc
     3061 acacgctggt caatccgcag ttcaacgcgg cgcttgcggc ggcgggtcga tcgagtcccg
     3121 aggtggccct gcagatcgtg ggcgagaacg atgccggcgt ggtcgtcaac ggcgcgcggt
     3181 tgcttgccac gctgggcccc catgcggacg agatcgaggt gtttccgtcc accctcctga
     3241 aggcctcgcc ggagaacgtg ccctttgcca tcgctttcac catccccatc gacaccaggg
     3301 gcctgcggct catctgtcgg gacacctacg acgcgggcaa gagccatttc gatgcgcctc
     3361 tggcatcccg attcgaggaa atggacgcgg tggtcatctt cgacaacgtg ttggtgccct
     3421 gggagcgtgt gttcatgtac caggcgccgc tgctgtgcaa ccgcgcgttt gcggagacca
     3481 acgccgtcgt gcacatgatg catcaggtgg cctgcgggaa gctggccaag gcggagttca
     3541 tcgtgggtct gctctgcgcc atggccaagg cctcgggcaa agacaaggat gccaacgtca
     3601 aaggccagat cgcggaagcg atgtggatag tcgaaaccgt ccgggccttt ctgttcagcg
     3661 ccgagcagca ggcagaaccc gatcgctggg gcctgtgggt acccaagcgc ctgcctctgg
     3721 acacgtcccg taatcttttc ccgaggcttt atccgaagct cgtggaaacc gtgcacatgc
     3781 tcggctctag ttcgctcatg gccacgccct gcgaggccga tttcgaaaac gccattgccg
     3841 gggacgtgga gcaatacttt cagctcacca atctggagag cagggaccgc gttgccctgt
     3901 tccggctggc gcacgacgtc gcggtctcag gattcggcaa ccgtcaggtg ctctacgagc
     3961 gctttttctt cggtccccag ccggtcatgg cctcgatcta ctacgatctt tacgataagg
     4021 acagcgtgat ggatcgcgtc acggaaatgc tcaagggctg ttgagacgac gggttccagt
     4081 ggcattgcgg atgcaacaca gggaattaac atgttaaata atcgctcatc ttggaaggcc
     4141 ggagccttgc gtggcgcaac cccgcaaatt cagtgagtct ctgcccatgc ttctgctccg
     4201 ggcccgcgag acctctatgg cctatttccg gccttccctg caggagcggg ggctcaccga
     4261 gcagcaatgg cgtgtgcttc gggcgttgca ggaatctggc gagctgtcgg cgtcggggct
     4321 ttcacacgcg tgcgtgattc tggcgcccag catgacgcgg atactgcgtc ggcttgccga
     4381 ggaggggctc gtttcgtcga agcggtcgcg ccgcgacggc agggagctca cggtgaggtt
     4441 gacccgcgcg ggcgagcgtc tgatcgacga gatcgcgccc atcagcgagc gtcagtacgc
     4501 gctcatccgc gaacggctgc aaccggaaaa gctggagcag ctccacagcc tgctgcacga
     4561 cttcgtggaa ctggatggcg agcacctgaa aggggccgca tcgggagaga cccaatgagc
     4621 caactgcttc acgaaaacct ggcaaagctc gaccggcacc tggcgcgttt ccgcaaccga
     4681 acgctggggc acctcatcga cggggaggaa gccgaaggcg tctcgggcga gacgtttacg
     4741 aacactaccc cggtggacga ctctccggtg aacgaggtgg cccggggtgc cgaggaagac
     4801 gtaaccagag cgacgcgggc agccgtaagg gcgtttggcg agtggcgtga catgccgggt
     4861 gcccgccggc gcggcctgct ccacgggatc gccgatgcca tcgaggcgcg ggctgaggag
     4921 attgccctgg tggagtcgta cgacaccggc cagccgctgc gtttcatgag cagcgcggcg
     4981 ctgcgcggag ccgaaaactt caggttttat gccgaccgag cgccggaagc ccgcaacggc
     5041 ctctccactc ccgccgatca gcatgtgaac tacagtgtcc gccaaccgat cgggccggtc
     5101 ggcgtcatca ccccttggaa cacgccgttc atgctctcca cctggaagat cgctccggct
     5161 ctggccgccg gctgcacggt ggtgcacaag ccggcagagt ggagccccct ctcggccagc
     5221 atactcgcgg agatctgtcg agatgccggg ctgcctgccg gcgtgctcaa cacggttcat
     5281 gggctaggcg aggaggctgg cgtggcgctc acggagcagc cggacatccg agccatcgct
     5341 ttcgtgggcg agtcccaaac gggccgcgcg atcatgagcc agggcgcgag cacgctcaag
     5401 cgcgtgcact tcgagctcgg cgggaagaat ccggtcatcg tgttcgacga cgccgatctc
     5461 gaacgcgcgc tggatgcctg cacgttcatg atctacagcc tcaacggcga gcggtgcacc
     5521 tcgtcgagcc gactgctcgt gcaggcgacg atctacgatg agttctgcgc ccgggtggct
     5581 gagcgagccg ggcagatccg tgtgggccac ccgctggatc ccgccaccga ggtgggaccg
     5641 ctgattcatc cgcgccacct cgaaaaggtg ctcgggtact gcgcgctggc gcagaccgaa
     5701 ggggctaccg tggcggtcgg gggcggccct ccgggggacg cggccgccgg gaactacgtg
     5761 gcgcccacgc tctttaccca cgcgcgcaac gacatgcgta tcgcccagga ggagatattc
     5821 ggtcccgtgc tgacggcgat ccctttcgac gacgaagccg aggccgttga gcttgccaac
     5881 caggtggagt tcggcctggc cggttacgtc tggacggggg acgtgggcag atcgcaccgg
     5941 cttgcgcgcg acctggatgc gggcatgatc tgggtcaact cggaaaacaa ccgtcatctg
     6001 ccgaccccgt tcggcggcat gaaggcaagc ggcatcggcc gggacggcgg tgactacagc
     6061 tttgatttct acatggaaac caagaacgtg tgcatatccc tcggcaagca ccgtgtgccc
     6121 agcctgggca aggggtgagg ctaccgtgag tctctactac gtgcagaagc ttctctacca
     6181 gctgaattgg gaccctcacg cccgccgacg ctacgaggag gatttcgacg agctgctggc
     6241 cgagtacgac ctcgacgccg aggagctcca cgccattcgg gagcccgaca tcgggcttct
     6301 ctacgtgatg ggcgtgaacg gccagatcct catgcactac gccgcgctgc gcggctacgc
     6361 gtgggacgag tacatcaagg ccatgcgtga cggccttgcc gagcacggag acgtccgggc
     6421 cgggctttac gtgaccacgg acggcagggg ggccgtctga tgggggtcgt gtttgccggc
     6481 gtggtcagcc acgctccggg gatcacgggg cgcgcagagc gcgccgatcc cgcgctccgg
     6541 gacgccctgt acgaaaattt ctaccgcctg gggcgcgagc tgaaggagtc gcgcccggat
     6601 gcgctgatcg tcatcgccgc cgagcacttc gccaacttct tcatggacaa catgcccgcc
     6661 tactgcatgg gaatggccga ttactacgaa gggcccatcg aagacgagca gtggctgggc
     6721 atcagtcggg tccggattcc gggcaacgcc gcgctggctg gcagcctgat cgaatccatg
     6781 ctggcggagt tcgatctggc ctacgctcag gagtggaagt tcgatcacgg cgtcatggtc
     6841 ccgctgcact ttctcacccc ggagtacgac attccggtga ttcccgtgaa cgtcaactgt
     6901 caggggccgc cgctgacgcc gctggcgcgg gcatacgatt tcggacgtgc tttgaggcgg
     6961 gcttgcgatg cgcgcccgga acgcatcgcc atcatcggca ccggcggcat ctcgcactgg
     7021 ccggcgacgc ctgactcggg aaagatcaac gaagcttggg atcgcgcctt cctggaaagc
     7081 tggctggcca atgaccgggc ggcgctcacc tctttcacgg acgaggagac ctatctcgag
     7141 gccgggcagg gcgggttcga gatccgcacc ttcatcgccg tttcgggagc cacacaagga
     7201 tgtgcggcgg aactctactt ctacgagccg atcccgatat tcgcggtggg ttgcaccgta
     7261 gcaagggttc acgtgtgacg acctgctgcc gcttcgcgct ccccgtgtgg ccataggcgg
     7321 gatgacggga aatagattga gcacgccttc ccaatgggtg taggctggct ggcgaacttg
     7381 tcacgacgga taaccaggag gggccat
//
LOCUS       DBNHKV010000317         2649 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_611550, whole genome shotgun sequence.
ACCESSION   DBNHKV010000317 DBNHKV010000000
VERSION     DBNHKV010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_611550"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /gene="cysD"
                     /locus_tag="AC16PP_09980"
     CDS             complement(<1..750)
                     /gene="cysD"
                     /locus_tag="AC16PP_09980"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HIR6481996.1"
                     /translation="MAYRAAESVARHNDSPPRRHPGQELSHLDRLEAESIHIMREVAA
                     QAENPVMLYSVGKDSSVMLHLARKAFYPSVPPFPLMHVDTRWKFKAMYDFRDQMARET
                     GMELIVYINPEGVEKDINPFDHGSALHTDVMKTQGLKQALDLHGFDVAFGGARRDEEK
                     SRAKERVFSFRTSSHRWDPKNQRPELWNLYNARKAKGESIRVFPLSNWTELDIWQYIM
                     AENIEIVPLYMAAKRPTVTRDGLLLMVDDDRF"
     gene            complement(1409..2242)
                     /locus_tag="AC16PP_09985"
     CDS             complement(1409..2242)
                     /locus_tag="AC16PP_09985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6481997.1"
                     /translation="MRIGVSLPVRELENDLGAIRAFAQLAEHLGLTHLRVPDQVLRQG
                     NKHLHEPLTLLAWIAGATERIELVPSVIVLPSRQTALVAKQAAEIDVLSNGRLRLGIG
                     VGGSPDEYAAMGMDYTTRGARCEEQMELLRLLWTREIVSFDGRWDKVNEAGLNPLPVQ
                     RPIPLWIGARGVPAQRILHRIGRLADGWFVLCTPEELPALDGAIAGAAREAGRDPGEI
                     GTEAGVAVVGPREAEWQDRVVGWRQAGLTHLCLRTLGGGLSPAEHMSKLEEVVDRLRE
                     L"
     gene            complement(2325..>2649)
                     /locus_tag="AC16PP_09990"
     CDS             complement(2325..>2649)
                     /locus_tag="AC16PP_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6481998.1"
                     /translation="RTPAYGLLVVISGLLGFAAGGFLPLLGAVVGSRFGPAAFGRVMG
                     LLGPFTTVAAIGPWIAGNLRDASGTYDDAWMLFVGIMVPAALAMAFMTPRPQPLALAS
                     AERAS"
BASE COUNT          468 a          917 c          821 g          443 t
ORIGIN      
        1 gaagcggtcg tcgtcgacca tcagcagcaa tccgtcgcga gtcaccgtcg ggcgcttggc
       61 ggccatataa agcggcacga tctcgatatt ctcggccatg atatattgcc agatgtcgag
      121 ctcggtccag ttggatagcg ggaagacgcg aatgctctcg cccttggctt tgcgggcatt
      181 gtagaggttc cacagctcag ggcgctggtt cttcggatcc cagcggtggc tcgacgtgcg
      241 gaacgagaac acgcgctcct tcgcccggct cttctcctcg tcccggcggg cgccgccaaa
      301 ggcgacgtcg aagccgtgga gatccagcgc ctgcttgaga ccctgggttt tcatcacgtc
      361 ggtgtgcaga gcgctgccgt gatcgaaggg gttgatgtct ttctccaccc cttcggggtt
      421 gatgtagacg atcagctcca tgcccgtctc ccgggccatc tggtcgcgga agtcgtacat
      481 ggccttaaac ttccagcgcg tgtccacgtg catgagcgga aacggcggta cgctgggata
      541 gaaggcctta cgggcgaggt gcagcatcac cgagctgtcc ttgcccaccg agtacagcat
      601 caccgggttc tccgcctggg cggcgacttc ccgcatgatg tggatgcttt ccgcctccag
      661 ccggtccagg tgtgaaagct cctgccccgg gtgccgacgg ggcggcgagt cattatggcg
      721 agcgacgctt tcggcggcac gataggccag cgtccccatc cgcgccttgc cgccggaagg
      781 cagctcgata tcgtgggagt ctgcccgttc caccagcgcc cgacaggcgc gcgctacctc
      841 cccatggggc cagatcagca gctgcttggg cgcttcccga ccgccggcaa gcacgtccag
      901 cgcgttcagg agcccatcga catccagccg cccgccatcc aggtagagcc agcgtctgcc
      961 catgcgcccg tcgttggcga agagcatgag ccccgccccg ggaacctcgt gagcctggag
     1021 aatcagggag agatgacgct tacgcttggc ttcggcttcc agagcatcca ccaccagccg
     1081 gaccggctcg cggccgagct cgtcgcgcac ctcgtccagc ttgagcgtgg cgggcagcag
     1141 gtcggcgctt tccagaagac gctccgtcga cctgagagac tgagcgctgc ccgattcgct
     1201 gagcagctcg tggacggcgg ccgtatccca ggcaccctgg gcctgggacc ggttcttgta
     1261 aaggctggcg ggtagctcca ctgggcgcgc acctcgaatg tcgtgatgcg caataatatt
     1321 cgaaatcagc gcgcctgcaa cgaattagac acggattaat gtaaccaata cgtacaaatc
     1381 tgcatattgc gacacccaac agcgggcctc aaagctcccg caaccgatcg accacttctt
     1441 ccagcttgct catgtgctct gccggcgaca gcccgccgcc cagggtccgc aggcagaggt
     1501 gcgtcagccc ggcctgccgc caccccacaa cccggtcctg ccactcggcc tcccgcggtc
     1561 ctaccacagc cacacccgcc tccgttccga tctccccagg atcacgtccc gcttcccgtg
     1621 ccgccccggc gatggcgccg tcgagcgcgg gaagctcctc gggcgtgcac agcacgaacc
     1681 agccatcggc aagccggccg atgcggtgaa gaatccgctg cgctggaacc ccgcgggcgc
     1741 cgatccacag gggaatcggg cgctgcacgg gcaaagggtt caagcccgcc tcgttgacct
     1801 tgtcccagcg gccgtcgaag ctcacgatct cccgggtcca cagcagtcgc agcagctcca
     1861 tctgctcctc gcagcgggcg ccacgcgtcg tgtagtccat ccccatggcc gcgtactcgt
     1921 ccgggctgcc gccgacgccg atgcccagcc gcaaccggcc gttagagagc acgtcgatct
     1981 ccgcggcctg cttggcaacc aaagccgtct gccgcgacgg caggacgatg accgacggca
     2041 cgagctcgat gcgttcggtc gcgccggcta tccaggcgag aagcgtcagg ggctcgtgca
     2101 gatgcttgtt accctgacgc agcacctggt cgggtacccg gagatgggtc agcccgaggt
     2161 gctcggccag ctgcgcgaac gccctgatag cacccagatc gttttcaagc tcccgcacgg
     2221 gtagcgatac gccgattcgc atgtggatcc tcccttagag ttctccctcg cgacgccgag
     2281 agggtcagtt ttcgagatga gtcagcctgt tgtgtccatc actttcacga cgcccgttca
     2341 gcgcttgcaa gagcgagcgg ctgagggcgc ggggtcatga acgccatggc cagagccgcc
     2401 ggaaccatga tgccgacgaa gagcatccag gcgtcatcgt aggttcccga ggcgtcgcgc
     2461 agattcccgg cgatccaggg accgatggca gcaacggtgg tgaacggccc aagcaggccc
     2521 atgacccgac cgaacgcggc cggcccgaag cggctgccca ccaccgcgcc gagcagcggc
     2581 aggaaaccgc ccgccgcgaa tcccagcagg ccactgatca ccaccagcag cccgtacgcc
     2641 ggggtccgg
//
LOCUS       DBNHKV010000318         4842 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_470358, whole genome shotgun sequence.
ACCESSION   DBNHKV010000318 DBNHKV010000000
VERSION     DBNHKV010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_470358"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="AC16PP_09995"
     CDS             complement(<1..157)
                     /locus_tag="AC16PP_09995"
                     /inference="COORDINATES: protein motif:HMM:NF014621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR6481999.1"
                     /translation="MRVVVQRVSSASVTVDDEAVASISTGLLVFLGVFPDDGDAQLHW
                     MADKVLGL"
     gene            complement(186..1988)
                     /gene="typA"
                     /locus_tag="AC16PP_10000"
     CDS             complement(186..1988)
                     /gene="typA"
                     /locus_tag="AC16PP_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR6482000.1"
                     /translation="MNTDHLRNVAIIAHVDHGKTTLVDRLLQQTGSLDRDQPERVMDS
                     NELERERGITILSKNTAIEYEGYRINIVDTPGHADFGGEVERILSMVDSVLLLVDAVE
                     GPMPQTRFVTEKAFAAGLSPIVVVNKVDRPGADPYRVIDQVFDLFDSLGASDEQLDFP
                     VVYTSALLGQAGPEPDALVDDMTPLLQSIIAHVPPPSVDADGPLQMQISSLDYSSYVG
                     VMGVGRIRRGRLARNTPVVVVDRNGRTRKARVLAVLGYHGLERIEREEAGAGDIVCVT
                     GVEEIGISDTICAPEHVEALPPLTVDEPTLTMMFCVNTSPFAGRDGSFLTSRQLRERL
                     LREASHNVALAVAETDDPDRFEVSGRGELHLSVLIETMRREGYEVAVSRPRVIMKEED
                     GRLVEPYETITIDVDDEHQGKVMESLGERRGELQEMHPDGHGRVRLRYRIPTRGLMGY
                     RPQFLSMTSGTGVMTYASAGYGPRFDGEIGRRTNGVLVSNAKGKAVAYALFNLQNRGR
                     LMIGPNDEVYEGMVVGVHSRDNDLTVNPMKEKKLTNIRASGSDENILLTTPIRLGLEQ
                     ALEFIDDDELVEITPTAIRVRKQWLKEHERKRAG"
     gene            2116..3171
                     /locus_tag="AC16PP_10005"
     CDS             2116..3171
                     /locus_tag="AC16PP_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482001.1"
                     /translation="MAEQRRAVALISGGLDSMLAAKVVMEQGVHVEGVNFFTGFCVEG
                     HTHAIREKDRNKPKRNNALWVAEQLGIRLHIIDVIDEYKDVLLNPKHGYGAHLNPCLD
                     CKVFMVRKALAWSFMEENGFDFIVTGEVIGQRPKSQRKETMPIIARESGADDRLLRPL
                     CAKHLPPTLPEREGWVDREALYDFHGRNRKPQFALARRFGFEDWSQPAGGCCFLTNAQ
                     YSAKLTDLWQAKGSRDYELDDIMLLKVGRHLRPRPHFKLIIGRELGENRFLQGYRHQF
                     TSLTAISHKGPLALIDGDAGADDIELAARILARYGQGRDAARVEVIAAEPGGAETRLS
                     VAPLAEADIGTDWHVGA"
     gene            3158..3442
                     /locus_tag="AC16PP_10010"
     CDS             3158..3442
                     /locus_tag="AC16PP_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR6482002.1"
                     /translation="MSELETLDARGLLCPLPVIRTQEKVKGLPPGTVVEIVATDPGVM
                     QDIPAWCRVHGHELISMTEGEEEAAAEGKADADRLIRGGREIRVRIKVRQ"
     gene            3642..>4842
                     /gene="glnA"
                     /locus_tag="AC16PP_10015"
     CDS             3642..>4842
                     /gene="glnA"
                     /locus_tag="AC16PP_10015"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR6482003.1"
                     /translation="MAENTLKLIKDNDVKWVDLRFTDPKGKEQHTTIPAHRLDADALN
                     DGFMFDGSSISGWKSINESDMILMPDDSTPVIDPFRDDTTLNLRCDIIEPSTMQPYDR
                     DPRSLAKRAEAYLTSTGIGDTAYFGPENEFFVFDDVRWKVEMSGASYSIGAEEAAWES
                     HSEFEGGNLGHRPSVKGGYFPVPPVDSAADLRSAMCSAMMAMGLDVEVHHHEVATACQ
                     NEIGARFNTLVRKADEVQIYKYCVHNVADAYGKTATFMPKPLVGDNGNGMHVHQSIAK
                     GDTNVFHGDEYAGLSKTALYYIGGIIKHARAINAFANASTNSYKRLVPGFEAPVLLAY
                     SARNRSASIRVPYIQGGNPRAYRVEVRFPDSTGNPYLTFAAMLMAGLDGIQNEIHPGD
                     SFDKDLYD"
BASE COUNT          952 a         1569 c         1482 g          839 t
ORIGIN      
        1 gcagtccgag aaccttgtcg gccatccagt ggagctgcgc gtcaccatcg tcggggaaca
       61 cccccaggaa gaccagcaac cccgttgaga tagacgcgac ggcctcgtcg tcgacggtca
      121 ccgacgcgct ggaaacccgc tgaacgacga cgcgcatcta aacacgcccc cgatcgggaa
      181 cggtttcagc cagcgcgctt gcgctcatgt tctttcagcc actgcttgcg gacgcgtatc
      241 gcggttggcg tgatctcgac cagctcgtca tcgtctatga actcgagcgc ctgctccagc
      301 ccgagcctga tcggcgtggt gagcagaata ttctcgtcgc tgcccgatgc gcgaatattg
      361 gtcagcttct tctccttcat ggggttgacc gtcaggtcgt tgtcgcggct gtgcacgccg
      421 accaccatgc cttcgtaaac ctcatcgttc ggaccgatca tcagccggcc gcggttctgc
      481 aggttgaaca gcgcgtaggc caccgccttg cccttggcgt tggagacgag caccccgttc
      541 gtgcggcggc cgatctctcc gtcgaagcgc ggaccgtacc cggcggaggc gtacgtcatg
      601 acgccggtgc ccgacgtcat ggacagaaac tgagggcgat agcccatcag cccgcgcgtg
      661 ggaatccggt agcgcagccg cacccgccca tggccgtccg ggtgcatctc ctgcagctcg
      721 ccgcggcgct caccaaggct ctccatcacc ttgccctggt gctcatcgtc cacgtcgatg
      781 gtgatcgtct cgtagggttc gaccaaccga ccatcctctt ccttcatgat gacccggggt
      841 ctcgagaccg ccacctcgta gccttccctg cgcatggtct cgatcagcac cgagaggtgg
      901 agctcgccgc ggcccgagac ctcgaaccga tccgggtcgt cggtttcggc caccgcaagc
      961 gcaacgttgt gagaggcttc cctgagcaat cgctcgcgca gctggcggct cgtgaggaac
     1021 gacccgtcgc gcccggcgaa gggggaggtg ttcacgcaga acatcatggt cagggtcggc
     1081 tcgtccacag tcagcggagg aagcgcttcg acgtgctcgg gcgcgcagat cgtgtccgaa
     1141 atgccgatct cctcgacgcc cgtcacgcac acgatgtcgc ccgcgcctgc ctcctccctc
     1201 tcgatccgct ccaagccgtg gtagccgaga acggccagca cccgtgcttt acgcgtccgg
     1261 ccattgcgat ccacaacgac caccggggtg ttgcgcgcga gccgtccgcg acgtatgcgt
     1321 cccacgccca tcacgccgac gtagctggag tagtcgaggg agctgatctg catctgcagc
     1381 ggcccgtccg cgtccacgct gggaggcgga acgtgggcga tgatgctctg cagcagcggg
     1441 gtcatgtcgt ctaccagcgc gtcgggctcg ggccccgcct gccccagcag cgccgaggtg
     1501 tagaccacgg gaaagtcgag ctgctcgtcg ctcgccccga ggctgtcgaa caggtcgaac
     1561 acctgatcga ttacccggta aggatcggcg ccgggacgat ccaccttgtt gaccacgacg
     1621 atggggctca accccgcggc gaacgccttc tccgtcacga agcgggtctg gggcatgggc
     1681 ccttcgacgg cgtccaccag caggaggacc gagtccacca tggagaggat gcgttcgact
     1741 tcgccgccga aatccgcatg acccggggta tcgacgatat tgatccggta gccttcgtac
     1801 tcgatggccg tgttcttcga cagaatggtg atgccgcgct cccgttcgag ctcgttgctg
     1861 tccatcaccc gctcgggctg gtcgcggtcg agcgagccgg tctgctgcag cagccggtct
     1921 acaagggtgg tcttgccatg gtcgacatgg gcgatgatgg cgacgttgcg caggtggtca
     1981 gtgttcattg atccattaca atgcgcggct tgaagcacca aagccgacag acggcatgaa
     2041 cggcgcctgc ggcgggggag catcaatcgg gcagcagcgg cagcggttat gccgcggcca
     2101 aaaaggtggc gaattatggc ggaacagcga agggctgtcg cgttaatttc gggcggtctc
     2161 gactccatgc tggcggcaaa ggtcgtcatg gagcagggcg tccacgtgga gggcgtcaac
     2221 ttcttcacgg gcttctgcgt ggagggccac actcacgcca tccgggaaaa ggaccgcaac
     2281 aagcccaagc gcaacaacgc gctgtgggtt gccgagcagc tcggtatcag gctgcacatc
     2341 atcgacgtca tcgacgagta caaggacgtc ctgctcaatc cgaagcacgg ctacggggct
     2401 catctgaacc cctgcctgga ctgcaaagta ttcatggtgc gcaaggcgct ggcctggagc
     2461 ttcatggagg aaaacggctt tgacttcatc gtcaccgggg aggtcatcgg tcagcgtccg
     2521 aaaagccagc gcaaggaaac catgccgatc attgcgcggg agtccggagc agacgaccgg
     2581 ctgctcagac cgctatgcgc caagcacctt cccccgacgc tgcctgaacg cgagggctgg
     2641 gtcgatcgcg aagccctcta cgacttccat ggacgcaacc gcaagccgca gttcgctctg
     2701 gcgcgccgct tcggcttcga ggactggtcc cagccggctg gagggtgctg tttcctcacg
     2761 aacgcgcagt actcggccaa gctgaccgat ctctggcagg caaagggatc gcgagactac
     2821 gagctggacg acatcatgct gctgaaggtc ggccgccacc tgcggccgcg gccccatttc
     2881 aagttgatca tcggccgcga gctcggcgag aaccggtttc tgcagggcta ccgccaccag
     2941 ttcacgagcc tgaccgccat cagccacaag gggccgctgg ccctcatcga cggagacgcc
     3001 ggcgccgatg acatcgagct ggcagcacgt atcctggccc gatacggtca aggcagagat
     3061 gcagccaggg ttgaggtgat cgccgcggag ccaggcggcg cggaaacccg cctgagcgtt
     3121 gcgccgctcg ccgaggccga tattgggacc gattggcatg tcggagcttg aaacattgga
     3181 tgctcggggg ttactgtgcc ccctgccggt gataagaacc caggaaaagg tcaaagggct
     3241 cccaccgggc accgtcgtcg agatcgttgc caccgaccca ggcgtgatgc aggacattcc
     3301 ggcctggtgc cgggtccacg ggcacgagct catctcgatg acggaaggtg aagaagaagc
     3361 ggccgccgaa gggaaggccg acgcagacag attgatccgg ggaggacgtg aaataagagt
     3421 caggatcaaa gtgcgccagt aatgctgata gtgcaccaaa atggtgcgta agcaccactt
     3481 tctcgcatcc attcgcacta tttctgagcg cctgtgagcc ggcactaacc tctgatatca
     3541 agaaaatggc cgtggcacgc ttactgcatt ccgcggtcgc acggcgcgga taccgcaaaa
     3601 gacattacta cgcacaccct agaaaggagc acgtacacac aatggccgaa aatactctga
     3661 aactcatcaa ggacaacgac gtcaagtggg tggatctgcg ctttaccgac ccgaagggaa
     3721 aggaacagca caccaccata ccggcgcacc ggctcgacgc cgacgcgctc aatgacggtt
     3781 tcatgttcga cggctcgtcc atctccggct ggaagtccat caacgagtcg gacatgatcc
     3841 tcatgcccga cgactccact cccgtcatcg acccgttccg ggacgacacg accctgaacc
     3901 tgcgctgcga catcatcgag ccctccacca tgcagcccta cgaccgggat ccgcgctctc
     3961 tggccaagcg ggcggaggct tacctgactt ccaccggaat cggcgatacc gcctacttcg
     4021 ggcccgagaa cgagtttttc gtgttcgacg acgtgcgctg gaaggtggaa atgagcggcg
     4081 cgtcctacag catcggcgct gaggaggcag cctgggaatc ccattccgag ttcgaaggtg
     4141 gcaatctggg ccaccgcccg tccgtgaagg gcggctactt ccccgtgccc ccggtggatt
     4201 ccgccgccga tctgcgctcg gccatgtgct cggcgatgat ggccatgggc ctggacgtcg
     4261 aggtgcacca ccacgaagtg gcaaccgcct gtcagaacga gatcggcgca cgcttcaaca
     4321 ctctggtgcg taaagccgac gaggtgcaga tatacaaata ttgcgtgcac aacgtggcgg
     4381 acgcttacgg caagacggcg accttcatgc ccaagccgct cgttggcgac aacggcaacg
     4441 gcatgcacgt tcaccagtcc atcgccaagg gggacaccaa cgtattccac ggcgacgagt
     4501 acgcggggct ctcaaagacg gctctgtact acatcggcgg gatcatcaag catgcaaggg
     4561 ccatcaacgc gtttgcgaat gccagcacca actcctacaa gcggctggtg ccaggcttcg
     4621 aggcccccgt gctcctggct tactccgccc gcaaccgatc cgcatcgatc cgggttccct
     4681 acattcaggg cggaaacccc cgggcctatc gggtcgaagt ccgcttcccg gacagcaccg
     4741 gaaacccgta cctgaccttc gccgccatgc tcatggccgg gctcgacggc atccagaacg
     4801 agatccatcc tggcgactcg ttcgacaagg atctctatga cc
//
LOCUS       DBNHKV010000319         2480 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_895665, whole genome shotgun sequence.
ACCESSION   DBNHKV010000319 DBNHKV010000000
VERSION     DBNHKV010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_895665"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..481
                     /locus_tag="AC16PP_10020"
     CDS             44..481
                     /locus_tag="AC16PP_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482004.1"
                     /translation="MAVYELNNGQLLRVARSIDGKLEQRYFSLIGLNKTQQRGVRREA
                     KAQDQAWQEAQEKAKAKRVREASTDRRHSTGVRGINLVRRPSPAFRVQVQTNGRSHVR
                     EFSVKRLGAEKAWRQATRFLAECRGYKTVPKAWTASVPRIPKG"
     gene            complement(478..1494)
                     /locus_tag="AC16PP_10025"
     CDS             complement(478..1494)
                     /locus_tag="AC16PP_10025"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF006046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIR6482005.1"
                     /translation="MDFVGSHILSVDQFTREDIEHVFRVADDMQPYADRERVTRVLQG
                     AILSNMFFEPSTRTRISFGSAFNMLGGEVRETTEVAASSLTKGESLYDTARVLSGYSD
                     VIVMRHPEAGSVAEFAKASRVPVINAGDGPNEHPSQALLDLYTIRKEMESRGRAIDDL
                     RIALVGDLRYGRAVHSLCKLLALFEHVHYKLVSPPELSLPADIVASLRAAGHDVEEHH
                     ELETGIDHVDILYVTRTQEERFPDQEEADRYRGLFRVNQAIYTKNCEPNTVIMHPLPR
                     DSRAQAQELDDDLIENPNLAIFRQTDNGVLIRMALFALVLDVAGQVHNHAQPVTWYTA
                     KRFR"
     gene            1683..>2480
                     /locus_tag="AC16PP_10030"
     CDS             1683..>2480
                     /locus_tag="AC16PP_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR6482006.1"
                     /translation="MSDLAHDEPDADIAQVQDAPLPLPFAFARRFGVLITGETGSREQ
                     ALVACKVQPSLTTLAEIKRVVRRELKLKMVGQDEFERLLTATYARDGSAARQMVEDMG
                     DEMDLASLADSVPETEDLLEQEDDAPIIRLINALLAEAIRENASDVHIETFEKDLIVR
                     FRVDGVMREVVKPKRQLAPLLVSRIKVMARLDIAEKRVPQDGRISLRIGGREVDVRVS
                     TMPASNGERVVLRLLDKQAGRLRLENLGMSNEGLGLLRDIVHKPHGIF"
BASE COUNT          453 a          766 c          822 g          439 t
ORIGIN      
        1 cggaacctgt ctttgcgagg ttcctcagcg aggagttagt attttggcgg tttacgaact
       61 caacaacggc cagcttctgc gggttgcccg cagcatcgac ggcaagctcg agcagcgtta
      121 cttcagcctc atcgggctga acaagaccca gcagcggggc gtgcgtcgcg aggcgaaagc
      181 acaggaccag gcctggcagg aggctcagga gaaggccaag gccaagcgcg tgcgggaggc
      241 ctctaccgat cgcaggcaca gcacgggcgt gcgcggcatc aacctggtgc gcaggccctc
      301 acccgccttc cgggtgcagg ttcagaccaa cggtcgctct cacgtccggg agttctcggt
      361 caagcggctc ggcgccgaga aggcctggcg ccaggcgacc cgctttctag cggagtgccg
      421 cggctacaag accgtgccga aggcctggac cgccagcgtg cctcgtatcc ccaaaggcta
      481 gcggaaccgc ttggccgtat accacgtcac cggctgagcg tggttgtgca cctggccggc
      541 cacatccagc accagcgcaa acaaggccat cctgatcaga accccgttgt ccgtctggcg
      601 gaaaatggcc agattggggt tttctatcag atcgtcgtcc agctcctgag cctgggcgcg
      661 ggagtcccgt ggcagcggat gcatgatcac cgtgttgggt tcgcagttct tcgtgtagat
      721 ggcctggttg acccggaaca gcccgcggta gcggtcggct tcttcctggt cgggaaaccg
      781 ttcttcctgg gtgcgcgtca cgtagaggat gtctacgtga tcgatgccgg tctccagctc
      841 gtggtgctcc tccacgtcgt ggccggccgc tctgagcgac gccacgatgt ccgccggcag
      901 ggaaagttcg ggcggggaca ccagcttgta gtggacgtgc tcgaaaagcg cgagcagctt
      961 gcagagggag tgcacggccc gtccgtagcg caggtcaccc accagcgcga tccggagatc
     1021 gtcgatggcg cggccgcggc tctccatttc cttgcggatg gtgtagagat ccagcagcgc
     1081 ctggctggga tgctcgttgg gaccgtctcc ggcgttgatg acgggcaccc ggctggcctt
     1141 ggcgaactcc gccaccgatc cggcctcggg atggcgcatc acgatgacgt cgctgtagcc
     1201 ggagaggacg cgggcggtgt cgtaaaggga ttcccccttg gtcagtgacg acgcggctac
     1261 ctcagtggtc tcgcgtacct cgccgccaag catgttgaac gccgagccga acgagatgcg
     1321 ggtccgggtg ctcggctcga aaaacatgtt ggagagaatg gcgccctgca ggacccgggt
     1381 gacccgttcc cgatccgcat aaggctgcat gtcgtccgcg acccggaaaa cgtgctcgat
     1441 gtcctcacga gtgaactggt ccaccgacag tatatggctg ccaacgaagt ccaacggcgt
     1501 cccccggctg aatttgcgca agtctacacc taccgcggcg tcacgggaaa acgagccgcc
     1561 gttcacgctt tgtgatgacc gtcacggaag caagggcagc gccacacggg ttggaatctt
     1621 tgggtttagc ggtgcgtgcc tgggttgcta tcattgggct gccccagtac gagtaccgcc
     1681 agatgagcga cttggcacat gacgagccgg acgccgacat agctcaggtg caggatgcgc
     1741 cgttgcccct gcctttcgcc tttgcccgcc gattcggcgt tctgatcacg ggagaaaccg
     1801 gctcccggga gcaggccctg gtagcctgca aggtccagcc gagcctcacc accttggccg
     1861 agatcaagcg cgtggtgcgg cgcgagctga agctgaagat ggtgggtcag gacgagttcg
     1921 aaagactgct cacggcgacc tacgcccggg acggctccgc cgcccgccag atggtcgagg
     1981 acatgggcga cgagatggat ctcgcaagcc ttgccgactc ggtcccggag accgaagacc
     2041 ttctcgagca ggaggacgat gcgcccatca tccggctcat caacgcgctg ctggccgagg
     2101 ccattcgcga aaacgcttcc gacgttcaca tcgagacgtt cgagaaggac ctcatcgtcc
     2161 ggttccgggt cgacggggtg atgcgggaag tggtcaaacc caagcgccag ctggcgcctc
     2221 tgctggtctc ccggatcaag gtcatggccc gcctggacat cgccgagaag cgggttcctc
     2281 aggacggccg gatctccctg cgcataggcg gccgggaagt cgacgtgcgg gtttccacca
     2341 tgccggccag caacggtgaa cgggtggtgc ttcggcttct ggacaagcag gcaggacggt
     2401 tgaggctgga aaatctgggc atgtcgaacg aagggctcgg gttgctgcgc gacatcgttc
     2461 acaagcctca cggcatcttc
//
LOCUS       DBNHKV010000320        13117 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1604733, whole genome shotgun sequence.
ACCESSION   DBNHKV010000320 DBNHKV010000000
VERSION     DBNHKV010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13117)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13117)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13117
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1604733"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..289)
                     /locus_tag="AC16PP_10035"
     tRNA            complement(214..289)
                     /locus_tag="AC16PP_10035"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(254..256),aa:Phe,seq:gaa)
     gene            complement(406..1296)
                     /locus_tag="AC16PP_10040"
     CDS             complement(406..1296)
                     /locus_tag="AC16PP_10040"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR6482007.1"
                     /translation="MPMGRPQAERVVDEALVRRLLEDQHPDLADLPLTHLDSGWDNVI
                     YRLGDKLTVRLPRREVAAALLHHEQTWLPRIADGLPLPVPSPVAMGRPTAYYPWPWSV
                     LPWFEGESADLSPPRAEQADRFADFLIALHRPAPADAPANPVRGVPLMAREANTRERM
                     GRLNSKSDALSPALESIWESALTAPQAGEACWLHGDLHAQNVLVNGTGSLTAIIDWGD
                     ITRGDPATDLAGIWAVFPTRRARQSILQAYRPDTALLDRARGWAFVFGVILTDSGNVN
                     SPRHAAAGRRILERLEADSG"
     gene            complement(1307..1579)
                     /locus_tag="AC16PP_10045"
     CDS             complement(1307..1579)
                     /locus_tag="AC16PP_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HIR6482008.1"
                     /translation="MSRTVTCRKYGEKLEGLDAPPMPGPKGQEIFETVSRRAWQEWQD
                     LQTMLINERALDMRDPEARKYLNAQRERFLDNEETDHAEGHVPRSS"
     gene            complement(1576..2643)
                     /gene="mutY"
                     /locus_tag="AC16PP_10050"
     CDS             complement(1576..2643)
                     /gene="mutY"
                     /locus_tag="AC16PP_10050"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIR6482009.1"
                     /translation="MRPDWFTKRVLKWFDLHGRHDLPWQEDNDPYRVWVSEIMLQQTQ
                     VTTVIPFYERFIARFPTVAALAGAELDEVLHHWTGLGYYARGRNLHKAARVIAHDLDG
                     RFPVGVEALSELPGIGRSTAGAISAIAQGVRAPILDGNVKRVLARFHAVAGYPGQTAT
                     QKSLWELAEAHTPNTRVADYTQAIMDLGATVCTRQPRCEVCPVAERCDALAQGATDDF
                     PGRKPKRSKPTRTARMFLIVAPDGACLLEQRPAKGLWGGLWIPPQRDPDCDVPAVLRE
                     FDIPESAVAESRVEPGFRHTFTHFHLDIEPVHVRLRARPLPGVRDGTDCLWYRREDDT
                     EVGLSAPAVALLDSLKEFELA"
     gene            complement(2646..4160)
                     /locus_tag="AC16PP_10055"
     CDS             complement(2646..4160)
                     /locus_tag="AC16PP_10055"
                     /inference="COORDINATES: protein motif:HMM:NF017021.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR6482010.1"
                     /translation="MVKRILMILLWIVLLGGGFVFYWIQDANRIKPELEQLIEEQAGI
                     PVTIGGDLSWRLFPPLTLQAEQIAASYEGQDWTAERLSLDIDVMTVLRTRDVNRWRVQ
                     SLTLTNTVGRDARTEIDIKSLTLREFELGKPSPLEISMVYTGEGAEPLPVKMTGLVTV
                     QSGPDRYEITNADLSTPYADGVCNLEASPASSSAPLPPATLEDLIPVATWRAFDWSGR
                     CDLSRLALDDLTFDSATVDLANTAANSATRITIPEFFGGTAILELQIDASGTPVRWIL
                     TPDLTGVDSQRLMAWLDQRLEWMAPLAYNGTLKFEGNTADALIASLSGETRFDGGRGA
                     ISIIKIKQPLLAVATMLQEPGRVSGWPDLWQYDRLEGVWRADRQHHTLDFALDNLSVI
                     ADGNYDPAADRLDMMAELTFRTLTEGKMFELNPVLMDLPIPVRCRGSLENPECRVDQK
                     AAQRIVASVLTSKEGNKAREKLEEKIDEEVPEEYRDAARGLLDLLGRSLQDKNQ"
     gene            4272..4670
                     /locus_tag="AC16PP_10060"
     CDS             4272..4670
                     /locus_tag="AC16PP_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482011.1"
                     /translation="MTVESFDPSAVDHTISDSRLARLLDAAGCLDEESFGLGTEEVRD
                     LAAVARHPESDWSERAGTLGDEEVLALIRLFTLAETRLSGWESGAASPVVPLAAALKR
                     RDAYPSDLNRWIRSNTANRFLPYGSLMDRL"
     gene            4729..5340
                     /gene="hisB"
                     /locus_tag="AC16PP_10065"
     CDS             4729..5340
                     /gene="hisB"
                     /locus_tag="AC16PP_10065"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIR6482012.1"
                     /translation="MATKTKPSGRSVTVSRDTQETRIAVSVDLDGSGASRLTTGLAFF
                     EHMLTQIARHGLIDLDIRADGDLEVDAHHTVEDVGIVLGQAIGQAVGDKAGISRYGHA
                     YVPLDEALSRAVVDFSGRPGLTYEVDFVRPRIGDFDVDLLREFFQGFVNHAQVTVHLD
                     NLRGENAHHQAETLFKAFGRALRMAVAPDPRMAGQVPSTKGSL"
     gene            5341..6084
                     /gene="hisA"
                     /locus_tag="AC16PP_10070"
     CDS             5341..6084
                     /gene="hisA"
                     /locus_tag="AC16PP_10070"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR6482013.1"
                     /translation="MLLIPAIDMKDGQCVRLRQGRMDDATVFSDDPLAMAAHWAEQGC
                     RRLHLVDLDGAFAGEPRNGALVAAIARELGEIPVQIGGGIRSLDIIERYLDAGVSQVI
                     LGTRAVQDPDFLASAAERHPGRIILGLDARNGMLATDGWDKTTQTSAVGFAMGASRLD
                     LAAIVYTDIERDGMLEGLNLAATVALAEAVETPVVASGGISCLDDLAALKAAFAGSSG
                     RLMGAITGRAIYAGTLDFRAGQALLDGSG"
     gene            complement(6094..7272)
                     /locus_tag="AC16PP_10075"
     CDS             complement(6094..7272)
                     /locus_tag="AC16PP_10075"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR6482014.1"
                     /translation="MRPITLVGIAISMVLGIGVGIGAYRAWLEPDVNGRHGQAFDEVL
                     RQVHEGYVDEVAKEKLLENALKGMLHGLDEHSDYLDHLSYEDLQAETSGQFGGIGIEL
                     NLVDGYFTVIRPITDTPASAAGLQPGDRLTEIDRESLKGKKMVDIISSLRGTPGTPVI
                     LGIRRDGGPKMEVSLIRAVIQDPSVDGRLLEPGYGYVRISQFQVSTGSRFTSAIRELA
                     HQNGESLRGLVLDLRDNPGGVLQSSVDVADALLEEGLIVYTEGRLPSSYLKYRATSGD
                     ILDGAPVVILINGGSASAAEVVAGALQDHGRAVVLGAKSYGKGSVQSVMPLSEDEAIK
                     LTTAYYYTPQGRSIEDTGIVPDVAVDEASGDDDDSGLLLGQALDLLKAEQGGGLHARL
                     "
     gene            complement(7301..8485)
                     /locus_tag="AC16PP_10080"
     CDS             complement(7301..8485)
                     /locus_tag="AC16PP_10080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR6482015.1"
                     /translation="MNSLPTTDWLPRSRAGRRAAGTRRLAIVVLSLLAPLSFSSEKED
                     AQRELEDVVGKLNALDVWLDEAEERRVRWLGEVKIKDREVASVSEAVDEATAALAVVI
                     RSLGKLEAEQRELQEKRERQATKIGEHLAAAYRLDGRDFVQLLLNQESPDSFDRMIRY
                     HRYFSSARLSALQEYQETLEALAANRLALEDRAAEQEARKAELAERQARLIEERDQRR
                     LLIAQLDAEAEDKAVQRQRLDTDRDRLERLLDELNRRAMELDGSAFVARKGSLPWPLK
                     GPLRSSFGRPRADGRMVWQGMLVAADEGSPVKAVFRGRLIFADWLRGFGLLAIINHGD
                     GYMTLYGHADSLTKTVGDWVEAGEVIARAGRSGGQQASGLYFEVREEGVPRDPLGWLA
                     KR"
     gene            complement(8482..10035)
                     /gene="gpmI"
                     /locus_tag="AC16PP_10085"
     CDS             complement(8482..10035)
                     /gene="gpmI"
                     /locus_tag="AC16PP_10085"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR6482016.1"
                     /translation="MGNPTLLVVLDGFGYSPSTQDNAIAQARTPNWDRFWNEKPHALI
                     SGSGADVGLPDDQMGNSEVGHMNLGAGRKVYQDLTRINRAIEQGEFARNRTLNRTLQR
                     LRKAGGRLHVMGLLSPGGVHSHEDQIFEFIRMAAAAEIQHIYLHAFLDGRDTPPRSAE
                     PSLARADALLAELGAGHVASVTGRYYAMDRDRRWDRIERAYNLLVRGEAPFHAANGRA
                     ALAAAYERDESDEFVQPTAVHAEGELPVRMLDGDAVVFMNFRADRARQITRAILEPGF
                     DGFQRRSTVALSDFVMLTRYADDIDTSCAFEPAVLSNTLGEYLANLGKTQLRIAETEK
                     YAHVTFFFSGGREDPFAGEERLLIPSPKVATYDLAPEMSARKVTDHLVEAIASKQHDL
                     IICNFANGDMVGHTGVFDAAVKAVETLDECLGRIETAIVEAGGQMLITADHGNVEQMR
                     DHGTGQAHTAHTCEPVPLVYIGPQSVKLNSGGTLSDVAPTLLELMHLEKPAEMTGHSV
                     AQLEERRSA"
     gene            10180..10596
                     /locus_tag="AC16PP_10090"
     CDS             10180..10596
                     /locus_tag="AC16PP_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR6482017.1"
                     /translation="MDQLFEFIVNHPVLVGAFVVLLVFFVRNETQRGGRSVSAQELVD
                     LVNREDAVVLDVRDKKDFDQGHIPHALNIPYASLDGRIAELNAHKEKPIVIACKMGQH
                     AGAAGTALRKQGFANVTRLKGGIAEWRNQNLPVVKA"
     gene            10560..11042
                     /gene="secB"
                     /locus_tag="AC16PP_10095"
     CDS             10560..11042
                     /gene="secB"
                     /locus_tag="AC16PP_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HIR6482018.1"
                     /translation="MAESEPAGGEGVARGQIRLERLYLKDASFESPRSPAVFGESWKP
                     EMQLDINTRTNPQEGDRFEVVLTVTLRAKLDDGRTAYIIEVQQAGLFVIQGAEDAVVH
                     RVLGTMCPNTLFPYVRETLDSLAVRGGFPAIQLAPVNFDALYEEALKQRAPPANPVQH
                     "
     gene            complement(11044..11511)
                     /gene="trmL"
                     /locus_tag="AC16PP_10100"
     CDS             complement(11044..11511)
                     /gene="trmL"
                     /locus_tag="AC16PP_10100"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HIR6482019.1"
                     /translation="MPLHVVLYQPEIPPNTGNVIRVCANTGARLHLVEPLGFEMDDSR
                     LRRAGLDYHEFACVDVHASLDDALDACGGGRCYAVSTRGSRRFSEITFTDGDVLLFGP
                     ETRGLPADVLETFPAAHVLRIPMRAGSRSLNLSNAVAVVVYEAWRQLGYAEGT"
     gene            complement(11515..12915)
                     /gene="ntrC"
                     /locus_tag="AC16PP_10105"
     CDS             complement(11515..12915)
                     /gene="ntrC"
                     /locus_tag="AC16PP_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HIR6482020.1"
                     /translation="MRNRVWVVDDDRSIRWVLERAMQQADIPIAAFASADHAMHHLQD
                     ERPVAVLTDVRMPGMDGMEFIGRIHEAYPELPVIIMTAHSDLDSAVNAYQSGAFEYLP
                     KPFDVDEAVAIVKRAMTFAQEEAPPPAPEQETAQEIIGQAPAMQEVFRAIGRLAHSNV
                     TVLIRGASGSGKELVARALHRHSPRAEGEFVALNMAAIPRELVESELFGHEKGAFTGA
                     NSRREGRFEQADGGTLFLDEIGDMPAETQTRLLRVLADGEFYRVGGHSPVKVDVRIIA
                     ATHQDLEVRVRDGSFREDLFHRLNVISVHVPTLAERRTDIPALASHFLEQAAEELGGE
                     PKIIRPDTMEYMMNLSWPGNVRQLENTCRWLTVMASGREIYLADLPPELGSDVVEQDT
                     NDSWEQALEAWADDLLREPGGLPLLDVAMPRFESVVIRAALRRTGGRKREAAELLGWG
                     RNTLTRKIAELGMTEL"
     gene            complement(12912..>13117)
                     /locus_tag="AC16PP_10110"
     CDS             complement(12912..>13117)
                     /locus_tag="AC16PP_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6482021.1"
                     /translation="MVDQIWFPMISGRADGTGLGLAITQSIVGHHNGIIECVSRPGDT
                     RFSVFLPVESNGEHIEQLENSQP"
BASE COUNT         2115 a         4232 c         4378 g         2392 t
ORIGIN      
        1 agggttaggc agttagggac aaaggtaaat agcgccgcct gcttcgggcg ctcatcagca
       61 acattcagcc ccccgctggt agaatcgacg ttgcgaagca gccgaccgcg gagcgtggcg
      121 gcatcagcga gagtggtgaa tcgaagtgaa gtgcgcgttt ggagctcaac aatggtgtcc
      181 gctggccact gcgtctcgac ctgcggtctc gtctggtggc caggggcaga atcgaactgc
      241 cgacacgcgg attttcagtc cgctgctcta ccaactgagc tacctggccg gaaagcttgc
      301 tggggcgggc cctttcactg cgtcctggga caggctttct tgtttggtcc ccagcaaagg
      361 ggcggtatta aaccgatctg acccttaaac tgcaaggcgt cgcggctagc cggaatccgc
      421 ctccagccgc tcgaggattc gcctgccggc ggcagcgtgc cgggggctgt tgacgttccc
      481 cgaatcggtc aggatgacgc cgaatacgaa ggcccacccc ctagctcggt cgagcagtgc
      541 ggtatccggc cgatatgcct ggagaatgct ctgccgcgca cggcgcgtgg ggaaaacggc
      601 ccagatgccg gccagatcgg tggccggatc gccccgggtg atgtctcccc agtcgatgat
      661 tgccgtaagg ctgccggtgc cgttcaccag cacgttctga gcgtgcaggt cgccgtgaag
      721 ccagcatgcc tcgccggcct gcggcgctgt gagcgccgac tcccagatcg attcgagggc
      781 gggggagagc gcgtcgcttt tcgagttcag gcgccccata cgctcgcggg tattggcctc
      841 gcgcgccatg agcggtaccc cccggaccgg attagccgga gcgtctgccg gagcggggcg
      901 gtgcagggcg atgagaaagt cggcaaagcg gtcggcctgc tcggcgcgcg gcggcgacaa
      961 atcggcgctc tccccctcga accagggcag aacgctccag ggccaggggt agtaggctgt
     1021 cggtctgccc atggcgaccg ggctcggcac gggaagggga aggccgtctg cgatgcgggg
     1081 caaccaggtc tgctcgtggt ggagcagcgc cgcagccacc tcccgccggg gcaggcgcac
     1141 cgtgagctta tcgcccagcc ggtagatcac gttgtcccag ccggagtcca gatgcgtgag
     1201 cggcagatcg gcgaggtccg ggtgctggtc ctccagcagc cgtcgcacca aagcctcgtc
     1261 cacgacccgc tctgcctggg gccgccccat gggcatggtc tgctgctcag gaagatcggg
     1321 gcacatggcc ctcggcgtgg tcggtctcct cgttgtccag aaaccgttcc cgctgggcgt
     1381 tgagatactt tcttgcctcg gggtcgcgca tgtccagcgc tcgctcgttg atcagcatcg
     1441 tctgcaggtc ctgccactcc tgccaggctc tgcgcgagac ggtttcgaag atctcctggc
     1501 ccttgggtcc gggcataggc ggcgcgtcga gaccctcgag cttttcgccg tacttgcggc
     1561 aggtgaccgt gcggctcatg ccagctcaaa ctccttaagg gaatccaata gtgcaaccgc
     1621 gggagcggac agtccaacct cggtgtcgtc ttctctccgg taccacaggc agtccgtgcc
     1681 gtcgcgaacg ccgggaagcg gtctcgcgcg aaggcggacg tgcacgggct cgatgtcgag
     1741 atgaaagtgg gtgaacgtgt ggcgaaaccc aggctcgacc cggctttcgg caacagcgga
     1801 ttccgggatg tcgaactccc ggagcaccgc tggaacgtcg cagtcggggt cccgctgcgg
     1861 cgggatccac aatccgcccc acaggccctt ggccggtcgc tgctccaaca ggcacgcccc
     1921 gtctggggcg acgataagaa acattctcgc cgtgcgcgtc ggcttggacc gtttcggctt
     1981 gcgcccggga aagtcatcgg ttgcgccctg ggcgagggca tcgcagcgct cggccacggg
     2041 gcagacctcg caccggggct ggcgagtgca cacggtagcg ccgaggtcca tgatcgcctg
     2101 ggtgtaatcg gcgacacgcg tgttcggggt gtgggcctcg gcgagctccc agagcgactt
     2161 ctgcgtggcc gtctggccgg ggtagcctgc cacggcgtgg aagcgggcta gcacccgttt
     2221 gacgttgccg tcgagaatcg gcgcgcggac tccctgggcg attgcggaga tggcgccggc
     2281 ggtagaacga ccgattcccg gcagctcgct cagcgcctcg accccgacgg gaaaccggcc
     2341 gtcgagatcg tgcgcgatga cccgggcggc cttgtggagg ttgcggcccc ttgcgtagta
     2401 gccgagcccc gtccagtggt gcaggacctc gtccagctcc gccccagcga gtgctgcgac
     2461 cgtcgggaaa cgggcaatga aacgctcgta gaacgggatc acggtggtga cctgggtctg
     2521 ctgaagcatg atctcggaga cccatacccg gtacggatcg ttgtcctcct gccacggcag
     2581 gtcgtgccgg ccgtgcaggt cgaaccactt cagaacgcgt ttcgtgaacc agtcgggcct
     2641 caccgttact ggttcttgtc ctgcaatgat ctgcccagga gatccagcag gccgcgggct
     2701 gcgtcccggt actcctcagg gacctcttcg tcgatcttct cctcgagctt ctctctcgcc
     2761 ttgttgccct ctttgctcgt cagcacggag gccacgatgc gctgggcggc tttctgatct
     2821 acccgacatt cggggttttc caggctgccg cgacagcgaa cgggaatggg caggtccatc
     2881 aggaccgggt tcagctcgaa catctttcct tcggtcagcg tcctgaaggt cagctccgcc
     2941 atcatgtcga gccgatcggc ggcaggatcg tagttgccgt ctgcgatgac cgacagattg
     3001 tccagagcga agtccagggt gtggtgctgc cggtccgccc gccacacgcc ctcgagacga
     3061 tcgtattgcc acagatcggg ccatccggag acccggcccg gctcctgcag catggtggcg
     3121 acagccagaa gcggctgctt gatcttgatg atggagatgg cgccccgccc accgtcgaag
     3181 cgcgtctcgc cggaaagcga ggcgatgagc gcgtcagccg tgttgccttc gaacttcagg
     3241 gtgccgttgt aggccagcgg cgccatccac tccaggcgct gatccagcca ggccatgaga
     3301 cgttggctgt ccaccccggt aaggtcgggg gtcaggatcc agcgaacggg cgtgccgctg
     3361 gcgtcgatct gcagctcgag gatggccgtg ccgccgaaga actcgggaat ggtgatccgc
     3421 gtggcgctgt ttgcagcggt gttggcgaga tcgaccgtgg cggagtcgaa ggtgaggtcg
     3481 tcgagcgcga ggcgcgacag gtcgcatcgg ccggaccagt cgaaggcgcg ccatgtggcg
     3541 acgggaatga gatcctcgag ggtcgccggt gggagcgggg cgctgctgct ggccgggctt
     3601 gcctcgaggt tgcagacgcc gtcggcgtag ggagtggata gatccgcgtt agtgatctcg
     3661 tatcggtcgg gtccggactg gacggtaacg agcccggtca tcttcacggg cagcggctcg
     3721 gctccttccc cggtatagac catcgagatc tccagcggcg acggcttccc cagctcgaat
     3781 tcccgcaggg tgagggattt gatgtctatc tccgtccggg cgtcgcggcc gacggtgttc
     3841 gtcaaagtca gcgactgcac ccgccagcgg ttcacatccc gggtacgcag cacggtcatg
     3901 acgtcgatgt ccaggctgag cctttcagcc gtccagtcct gaccctcgta gctcgcggcg
     3961 atctgctctg cctgcagtgt caggggcggg aacagacgcc agctcagatc cccgccgatg
     4021 gtgacgggaa tgccggcctg ctcttcgatg agctgctcca gctccggctt gatccggttg
     4081 gcgtcctgga tccagtaaaa gacgaagccg ccaccgagca gcacgatcca caggaggatc
     4141 atgagaatgc gtttgaccat gtggtgttgc cgtgagtctg ggaagtgagg acgattataa
     4201 ggatttcggg ctggccggca gctgaacggc gcgtttgcta tagtctcgcc cccgcagacc
     4261 atctggccgc catgaccgta gaatccttcg acccctcagc cgtggaccac acgatttccg
     4321 attcgcgtct cgcgcgcctg ctcgatgcgg ccggttgcct cgacgaggag agcttcggtc
     4381 tcggtaccga ggaggtgaga gatctcgcgg ccgttgcccg ccatccggag tcggactggt
     4441 ccgagcgggc ggggacgctg ggagatgagg aagtcctggc cctgatcagg ctgttcacgc
     4501 tggcggaaac ccgcctgtcg ggctgggagt ccggagcggc atcgcccgtg gtcccgctgg
     4561 cggcggcgct caagcgccgc gatgcctacc cgtcagatct caaccggtgg atcaggagca
     4621 acacggcaaa ccgctttctg ccctacggga gcctgatgga ccggctgtag tcgtcgggtt
     4681 cgagtccgta tacttgcgcg gcctcactga agcggaaggc agttgatcat ggccacgaaa
     4741 accaagccca gcggtcgctc ggttaccgtc tcccgggata cccaggagac gcggatcgcc
     4801 gttagcgtgg atctggacgg ctcgggcgcg tccaggctga ccacggggct cgcctttttc
     4861 gagcacatgc tcacgcagat tgcccgtcat gggctcatcg atctcgacat ccgcgctgac
     4921 ggcgacctcg aggtggatgc ccaccacacg gtggaggacg ttggcatcgt cctgggccag
     4981 gccattggtc aggccgtcgg cgacaaggcc ggcatctcgc gctacgggca tgcttacgtt
     5041 cccctggatg aagcgctctc ccgtgccgtg gtggactttt ccggccgccc gggcctgacc
     5101 tacgaggtgg atttcgtgcg gccccgaatc ggcgatttcg acgtcgacct ccttcgcgag
     5161 tttttccagg gattcgtcaa ccacgctcag gtgacagtgc atctggacaa tctgcgcggg
     5221 gagaacgccc accatcaggc cgaaacgctt ttcaaggcgt tcggccgggc gctgcgcatg
     5281 gccgtcgccc cggatccgcg catggccggg caggtgccgt ccaccaaagg cagcctctag
     5341 atgctcctga tcccggccat cgacatgaaa gatggccagt gcgtgaggct tcgccagggt
     5401 cggatggacg acgccacggt gttctcggac gatccgctgg ccatggccgc gcactgggcc
     5461 gagcaggggt gtcgccggct gcatctcgtg gatctcgacg gcgcctttgc cggcgagccg
     5521 cgcaacggcg ctctggtggc cgccatcgcc cgtgagctcg gtgagatccc ggtgcagatc
     5581 ggcggcggca ttcgcagcct ggacatcatc gaacgatatc tggacgccgg ggtctcccag
     5641 gtcattctgg ggacccgggc cgttcaggac cccgatttcc tggcgtccgc agccgaacgg
     5701 caccctggcc ggatcatcct tggcctcgac gctcgaaacg gcatgctcgc caccgacggc
     5761 tgggacaaga cgacccagac cagtgccgtc ggctttgcga tgggcgcctc gcgactggat
     5821 cttgcggcca tcgtgtatac ggacatagag cgggacggca tgctcgaggg gctcaacctg
     5881 gcggcgaccg tggcccttgc cgaggccgtc gagacccccg tcgtcgcctc gggcggcatc
     5941 agctgcctcg acgatctggc agccctgaaa gccgcctttg ccgggagctc gggaaggctg
     6001 atgggagcca tcacgggtcg cgccatctat gcgggcaccc tggattttcg ggctggacaa
     6061 gcccttctcg acggctcggg ttgaaggcgc gcgctacagg cgcgcgtgca gccctccgcc
     6121 ctgttcggct ttgagcaggt cgagggcctg gccgagcagc agcccggagt cgtcatcgtc
     6181 gccagaagcc tcatcgacgg caacatcggg cacgatgccg gtgtcctcga tggatcgccc
     6241 ctgaggggtg tagtagtagg cggtggtcag cttgatcgcc tcatcttccg acagcggcat
     6301 taccgactga accgagccct taccgtaact tttcgctccc aggacgacgg ccctgccgtg
     6361 gtcctggaga gcgcccgcga ccacctcggc tgcggatgcc gagccgccgt tgatcagaat
     6421 caccaccggg gcgccatcga ggatgtcacc gctggtcgcc cggtatttca gatagctgga
     6481 aggcaggcga ccctcggtgt acacgatgag gccctcctcg agcagggcat ccgcgacgtc
     6541 cacggaggat tgcaggaccc cacccgggtt gtcccgcagg tccagcacca ggccgcgcag
     6601 gctttccccg ttctggtgcg ccagctcacg aatggccgag gtgaaccggc tcccggtgct
     6661 cacctggaac tgcgagatcc ggacgtaacc gtaacctggc tcgagcagcc ggccgtccac
     6721 gctgggatcc tgtattacgg cccggatcag gctgacctcc atcttcgggc cgccgtcgcg
     6781 gcgaatgccc aggatgaccg gcgtgccggg tgtcccgcga aggctggaga tgatgtccac
     6841 catcttcttg cccttgagcg actctctgtc gatctcggtc agcctgtcgc cgggctggag
     6901 ccccgcggcg ctggcgggcg tgtcggtgat cggccggatt acggtgaaat agccgtcgac
     6961 caggttgagc tcgatgccga tgccgccgaa ctgcccgctg gtttccgcct gcagatcctc
     7021 gtagctgagg tggtccagat agtcggaatg ctcgtcgaga ccgtggagca ttcccttgag
     7081 ggcgttttcg aggagctttt ccttggccac ctcgtccacg tagccttcgt gcacctggcg
     7141 cagcacctcg tcgaaggcct ggccgtgccg gccattcaca tcgggctcca gccaggcccg
     7201 gtaagcgccg atgccgacgc cgatcccgag gaccatgctg atcgcgatgc cgaccagagt
     7261 tatgggacgc aagttcatac cggggagttt ggcaccagca tcagcgtttg gccaaccacc
     7321 cgagggggtc gcggggcacg ccttcctcgc gcacctcgaa gtacaggccg ctggcctgct
     7381 ggccgccgct gcggccggcg cgggcgatga cctcccccgc ctcgacccag tcgcccacgg
     7441 ttttcgtgag cgagtcggca tggccgtata gcgtcatgta accgtcgccg tggttgatga
     7501 tggcgagcag tccgaagccg cgcagccagt cggcgaaaat caaccggccg cgaaaaacgg
     7561 cctttaccgg cgagccttcg tccgcggcca ccagcatccc ttgccagacc attctcccgt
     7621 cggcccgtgg ccgtccgaac gatgagcgca ggggaccctt gagcggccag ggaagtgatc
     7681 ccttgcgggc aacgaaggcg gacccgtcca gctccatggc gcgtcggttg agctcgtcca
     7741 gcagacgttc gagccggtcg cggtcggtgt ccagccgctg ccgctggacg gctttgtcct
     7801 ctgcctcggc atccagctgc gcgatcagaa gcctgcgctg atcgcgctcc tcgatgagcc
     7861 tggcctggcg ctccgcaagc tccgccttgc gggcttcctg ctcggccgca cgatcttcca
     7921 gggccagcct gttggcggcc agcgcctcga gagtttcctg gtactcctgc agggcagaca
     7981 ggcgggcgct gctgaagtag cggtggtaac ggatcatccg atcgaacgag tccggggatt
     8041 cctgattgag caagagctgc acgaagtcgc gaccgtccaa acggtacgcg gcggcgaggt
     8101 gctcgccgat cttggttgcc tgccgctccc gcttctcctg cagctcgcgc tgctccgcct
     8161 cgagcttgcc cagggaccga atcaccacgg ccaacgcggc ggtcgcctcg tcgacggcct
     8221 cgctcaccga ggccacctcc cggtccttga tcttgacctc tccgagccag cgcacgcggc
     8281 gctcctcggc ttcatcaagc catacgtcca gggcgttgag cttgcccacg acgtcctcca
     8341 gctctctctg ggcgtcttcc ttctcgctgg agaagctcaa cggcgccagg agagaaagca
     8401 ccacgatcgc aaggcgacgc gtgccggccg cccggcgccc ggcgcggctc cgcggtagcc
     8461 agtcggtcgt cgggagactg ttcaagcgga gcggcgttcc tctagctgag cgacggagtg
     8521 cccggtcatt tctgccggtt tttccaggtg catgagctcg aggagggtgg gtgctacgtc
     8581 ggagagggtt cctccggagt tcagcttgac cgattgcggt ccgatgtaga cgaggggcac
     8641 gggctcacag gtgtgggcgg tgtgagcctg gccggtgcca tggtcgcgca tctgctccac
     8701 gttgccgtgg tcggctgtga tgagcatctg cccgcctgcc tctacgatgg cggtttcgat
     8761 ccggcccagg cactcgtcca gtgtctccac ggccttcacg gccgcgtcga acacgccggt
     8821 gtgaccgacc atgtcgccat tggcaaagtt gcagatgatc aggtcgtgct gcttcgacgc
     8881 tatcgcttcc accaggtggt cggtgacctt gcgcgcgctc atctcgggcg ccaggtcgta
     8941 cgtggccact ttcggtgaag ggatgagcag gcgttcctcg ccggcgaagg gatcctctct
     9001 cccgccggag aagaagaaag tgacgtgagc gtacttctcg gtctcggcga tgcgcagctg
     9061 ggtcttgccc aggttcgcca ggtactcgcc gagcgtgttg gacagcacgg ccggctcgaa
     9121 cgcgcacgag gtgtcgatgt cgtcggcgta ccgggtgagc atgacgaagt cggacagcgc
     9181 taccgtggag cggcgctgga aaccgtcgaa gcccggctcc aggattgccc gggtgatctg
     9241 gcgggctcgg tcggcgcgga agttcatgaa gaccacggcg tctccgtcga gcatccggac
     9301 cggcagctcg ccctccgcgt gcacggccgt gggctgcacg aactcgtcgc tttcgtcccg
     9361 ctcgtacgcg gccgccagcg ctgcgcgccc gtttgccgca tgaaagggag cctctccccg
     9421 gaccagcagg ttgtacgcgc gctcgatgcg gtcccagcgc cggtctcggt ccatggcgta
     9481 gtagcgcccg gtaacagagg ctacgtgacc tgcgccgagc tcggcaagca gagcgtcagc
     9541 ccgtgcgagg gacggctccg cgctgcgcgg cggtgtgtcg cgcccgtcca gaaaggcgtg
     9601 cagatagatg tgctgaattt ccgccgccgc ggccatccgg atgaactcga agatctgatc
     9661 ttcgtggctg tgcaccccgc ccggggacaa caggcccatg acgtgcaggc gcccgccggc
     9721 ttttctgaga cgctgcaaag tcctgttgag agttctgttg cgggcgaact cgccttgctc
     9781 gatggcgcgg ttgatccggg tcaggtcctg atagaccttg cgaccggccc cgaggttcat
     9841 gtgccccact tccgagttcc ccatctggtc gtccggaagg ccaacgtccg cccccgagcc
     9901 cgagatcagc gcatgaggtt tctcgttcca gaagcgatcc cagttgggcg tgcgcgcctg
     9961 ggcaatggcg ttgtcctgcg tggacgggct gtagccgaaa ccgtccagaa ccaccagcag
    10021 cgtcggattt cccatgggtt ttcggccaaa cctcctcgag ttgcggggtt ggggtagcgg
    10081 ccgggagttt aacagaatcc atggctacga gaggttgaac cggtgtgtat aatcgcgcgg
    10141 tttccagcca cgtcggagcc cggagcagag ggcgcagaga tggatcagct atttgagttc
    10201 atcgttaatc atcccgttct cgtgggggct ttcgtggtgc tgctggtgtt cttcgtgcgc
    10261 aacgagaccc agcgcggcgg ccggtccgtc tcggctcagg agctggtcga cctggtcaac
    10321 cgggaggacg ccgtggtgct cgacgtgcgg gacaagaagg atttcgacca gggccacatt
    10381 ccccacgcgc tcaacatccc ctacgcgtcc ctggacggcc gaatcgccga gctgaacgct
    10441 cacaaggaaa agccgatcgt gatcgcctgc aagatgggtc agcatgccgg tgctgcgggc
    10501 acggcgctgc gcaagcaggg gttcgcgaac gtcacacggc tcaaaggagg catcgccgaa
    10561 tggcggaatc agaacctgcc ggtggtgaag gcgtagcccg gggccagatt cgtctggagc
    10621 gcctgtacct caaggacgct tcgttcgaat cgccgcgctc gccggcggtg ttcggcgaaa
    10681 gctggaagcc cgagatgcag ctggacatca acacccgaac caatccccag gaaggggatc
    10741 gtttcgaggt ggtattgacc gtgaccctgc gcgccaagct cgatgatggg cgcaccgcct
    10801 acatcatcga agtccagcag gccgggttgt tcgtgattca gggcgctgaa gacgccgtgg
    10861 tgcaccgggt gctgggaacg atgtgtccga acacgctgtt tccctacgtg cgggaaactc
    10921 tggactcgct ggcggttcgc ggcgggtttc ccgccattca gctcgcgccc gtgaacttcg
    10981 atgcgcttta cgaggaagcg ctcaagcagc gggcacctcc ggcaaacccc gtccagcact
    11041 gatctaagtt ccctcggcgt agccgagctg tctccacgcc tcgtagacca caacggcgac
    11101 ggcgttggag aggttcaggc tgcgcgagcc cgcccgcatg ggaattcgca gcacgtgcgc
    11161 cgctggaaac gtctcgagca cgtccgccgg cagcccccgg gtctccggac cgaagagcag
    11221 cacgtcgccg tcggtgaacg tgatctccga gaagcgtctg gaaccgcgcg tgctcaccgc
    11281 gtagcaccgt ccgcccccgc aggcgtcgag cgcgtcgtcc aggctggcgt gaacgtcgac
    11341 gcaagcaaac tcgtgatagt cgagccccgc ccgccggagc cttgagtcgt ccatctcgaa
    11401 accgagcggc tcgaccaggt gcagacgggc gccggtgttc gcgcagacgc ggatgacgtt
    11461 gccggtgttt ggcggaatct ccggctggta cagcacgacg tgaaggggca tggctcagag
    11521 ttcggtcatc ccgagctcgg caatcttccg ggtgagcgtg ttgcgtcccc agccgagcag
    11581 ctcggccgcc tcgcgcttgc gcccgccggt gcgcctgagc gcggctcgga tgaccacgct
    11641 ttcgaaacgc ggcatcgcca catccagcag cggcagcccg ccgggctcgc gcagcaggtc
    11701 gtcggcccag gcttccagcg cctgttccca gctgtcgttt gtgtcctgct cgaccacatc
    11761 ggagccgagc tccggcggca ggtcggctag atagatttca cgtccggagg ccatcacggt
    11821 gagccagcgg caggtgttct cgagctggcg cacgttgcct ggccagctca gattcatcat
    11881 gtactccatc gtgtcgggac ggattatctt gggctcgccg cccagttcct ccgccgcctg
    11941 ctccaggaaa tggctggcca gcgcgggaat gtccgttcgc cgttccgcca gagtcggcac
    12001 gtggacgctg atgacgttca gccggtgaaa gagatcctcc cggaagctgc cgtccctcac
    12061 ccgaacttcg aggtcctggt gggttgctgc gatgatgcga acgtccacct tgaccgggga
    12121 gtgtccgccc actcgataga actccccgtc ggccagcacg cgcagcagcc gggtctgggt
    12181 ttctgcgggc atgtcgccga tctcgtccag gaacagggtg ccgccgtcgg cctgctcgaa
    12241 acgcccttcg cgtcgcgagt tggcgcccgt gaaagcgccc ttctcgtggc cgaacagctc
    12301 tgattccacc agctccctcg gtatggccgc catgttcaaa gcgacgaatt cgccctccgc
    12361 gcgggggctg tggcggtgca gcgcacgggc gaccagctcc tttcccgacc ccgatgcgcc
    12421 gcgtatcagg accgtgacgt tggagtgtgc cagccggccg atggcgcgga agacctcctg
    12481 catggcgggc gcctggccga tgatttcctg ggcggtttcc tgttcgggcg ccggcggcgg
    12541 cgcttcttcc tgggcaaagg tcatggcccg tttgacgatg gcgacggctt cgtccacgtc
    12601 gaacggtttg ggcagatact cgaacgcgcc gctctggtag gcgttgacgg cgctgtcgag
    12661 gtcggaatgg gccgtcatga tgatgaccgg cagctccggg taggcctcgt ggatgcgacc
    12721 aatgaactcc atgccgtcca tgccgggcat tcgcacgtcg gtaaggacgg cgacgggccg
    12781 ctcgtcctgc aggtgatgca tggcgtgatc ggccgaggcg aaagcggcaa tgggaatgtc
    12841 agcctgctgc atggcccgct ccagcaccca gcgtatggac cggtcgtcgt cgacgaccca
    12901 gactcgattt ctcacggctg cgaattctcc agttgttcga tgtgctcgcc gttgctctcg
    12961 acgggcagga ataccgaaaa gcgggtgtcc ccggggcggc tgacgcactc gatgatcccg
    13021 ttgtgatggc caacgatgga ttgggtaatg gccagcccca gaccggtgcc gtcggctcgc
    13081 ccgctgatca tcgggaacca gatctggtcc accatgc
//
LOCUS       DBNHKV010000321         3297 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_541689, whole genome shotgun sequence.
ACCESSION   DBNHKV010000321 DBNHKV010000000
VERSION     DBNHKV010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_541689"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1266
                     /locus_tag="AC16PP_10115"
     CDS             196..1266
                     /locus_tag="AC16PP_10115"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR6482022.1"
                     /translation="MVQTDVRKLEPMELPLPEIDEDSALLKLEACGICGSDHEQYEGL
                     LRTPMPVVPGHEPLGTIAKIGDRAATRWGVDQGDRVAVETMISCRHCRPCLSGSYHLC
                     DHRLIYSYVPVSEAPGLWGGYSQYMYLHPNSIVHRVSPELPAEIGVMFNPLGAGFRWA
                     VELPATRPGDTVVILGPGQRGLASVIACREVGAGTIIVTGLSADEKKLQLAREFGADY
                     TIDVDSEDTVKRVKAITDGRGADVVVDVSAYATKPVSDALSLVRPGGTVVLAGVKGFK
                     AVEDFVSDLVVLKEINIKGAIGVTSSGYRSAIRLLESGRVPVERMHTHDFALADAELA
                     IRTLAREVPGDESVHSCLIPPH"
     gene            1284..2267
                     /locus_tag="AC16PP_10120"
     CDS             1284..2267
                     /locus_tag="AC16PP_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6482023.1"
                     /translation="MKYGLILGSPSIQGQIDTARRAEAAGFESVWTIEFFNMHGLVRL
                     AAVATATERVKLGTAIAYAFMRTPMLAASAAMDIDEISGGRMILGLGSGTERMNRDWY
                     SMPFDDPPAPRIKDAIGLVRAAFAAQKGGGLVYEGPYYQVRIPAYARPGAVRESIPVC
                     LAAVNRGMIRAAAAVADGLIGHPVYTRRYITDVVLPELENGSCELIPYIITAVADTTE
                     QARNEARGQIAFYYTTRLYHSILEPHGWRDTGEAIAAAFRKGDFSAMADAVSDEMVDA
                     IAIAGTPDEVRDQITQWEGLADHVILYSPSVGMRGGRVQENLDAIVDTFAA"
     gene            2276..2716
                     /locus_tag="AC16PP_10125"
     CDS             2276..2716
                     /locus_tag="AC16PP_10125"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6482024.1"
                     /translation="MAVASNISFSEKVAQGFMRAPSGAGGGLPEFLGVKITDAGPGTM
                     TGQFEVRPELLTPIGNMHGGVLSAFCDHMLGCVCYPAMKPGQWAATTEFKINLTAPVT
                     QGVVEARAEIVNMSRTQAVVRLDLTNEGRVVAMAQGTVTVRDPR"
     gene            2798..3286
                     /locus_tag="AC16PP_10130"
     CDS             2798..3286
                     /locus_tag="AC16PP_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482025.1"
                     /translation="MMIRHGWLLLLLLMGCSSTVVLDRDVPSGFDLDGEWVVDAAASE
                     VAPGTRRLRAQGIGISLVAHDFPVLRCERMTIEQSADSMGIEYDGASYRDVSWGVRQR
                     GLWEVNAGWEEGVFRILSKARDAKAEEMMTLADSGRRLIVQVVVESEGDTIDITRQFV
                     RR"
BASE COUNT          554 a         1008 c         1157 g          578 t
ORIGIN      
        1 cgtgctcggc cgcgctgatc gcggcgcaaa gggccgcgtt gccgccgccg acgacgatga
       61 cggtgggggt tcttgccatt gctctgtcag gccgttgccc gtagtctcgt ggagactatc
      121 acgggaggag gcgcgaccga gcgcttcgag cgagggggag ccgcaccgtt gacgttgccg
      181 agaatgtcgt tcgcgatggt tcagaccgac gtgcgcaagc tcgagcccat ggagctcccg
      241 ctgcctgaaa tcgacgaaga ttcggcgctg ctgaagctgg aagcctgcgg tatctgcggc
      301 agcgatcacg agcagtacga gggtcttctg cgcacgccca tgcccgtggt tcccggtcac
      361 gaacccctgg gcaccatcgc caagatcggt gaccgggcgg cgactcgctg gggcgtcgat
      421 cagggcgatc gggtcgcggt ggagacgatg atctcctgcc gtcactgccg gccgtgcctg
      481 agcggctcgt atcacctctg cgaccaccgg ctcatctact cgtacgtgcc tgtgagcgaa
      541 gcgccggggc tgtggggcgg ctactcacag tacatgtatc tgcatccgaa cagcatcgtg
      601 catcgggtga gcccggagct gccggccgag atcggcgtga tgttcaatcc gcttggcgcg
      661 ggcttccgct gggcggtgga gctgccggcg acccggccgg gagacacggt ggtcattctc
      721 ggccccggcc agcgggggct tgccagcgtg attgcctgcc gggaggtagg ggccgggacc
      781 atcatcgtga cgggcctttc cgcagacgag aagaagcttc agctcgcccg ggagttcggg
      841 gcggattaca ccatcgacgt ggacagcgag gacacggtaa agcgggtcaa ggcgatcacg
      901 gacgggcgcg gcgccgatgt ggtcgtcgac gtgagcgcat atgcgacgaa acccgtgtcc
      961 gatgcgctgt cgctggtccg tcccggcggc acggtggtgc tggccggcgt gaagggcttc
     1021 aaggcggtgg aggatttcgt ctcggatctc gtggtgctca aggaaatcaa catcaagggg
     1081 gccatcggcg taacttcgtc cggttatcgc agcgccatcc gcctgctgga gtccgggcgc
     1141 gtgccggtgg agcgcatgca cacccatgac ttcgcgctgg cggacgcgga gcttgcgata
     1201 cgaaccctgg ctcgagaggt tcctggcgac gagtccgttc actcctgcct gatccccccg
     1261 cactgacgcg aagaggacac gtcatgaaat acggcctgat tctgggatcg ccctccatcc
     1321 agggacagat cgacacggct cgccgggcgg aagccgcggg cttcgaatcc gtttggacca
     1381 tcgagttctt caacatgcac gggctggtgc gccttgccgc cgtggccacg gcgacggagc
     1441 gcgtcaagct gggcaccgcc atcgcgtatg ccttcatgcg cacaccgatg ctcgccgcat
     1501 ctgccgccat ggacatcgac gagatttccg gcgggcgcat gattctggga ttgggcagcg
     1561 gcaccgagag gatgaaccgc gactggtact ccatgccgtt cgacgacccg ccggcgccca
     1621 ggatcaagga cgccatcggc ctcgtgcggg cggcgtttgc cgctcagaag ggcggggggc
     1681 ttgtctacga aggcccttac tatcaggtca ggattcccgc gtatgcgcgc cccggcgccg
     1741 tgcgggaatc gatcccggtg tgcctcgcgg ccgtgaaccg gggcatgatc cgcgctgcgg
     1801 ccgccgtggc ggacgggctg atcggccacc cggtctacac ccgcaggtac atcaccgacg
     1861 tggtcttacc ggaactggag aacggctcgt gcgagctgat cccttacatc atcacggcgg
     1921 tggcggacac gacggagcag gcgcgcaacg aagcgcgcgg gcagatcgcc ttttactaca
     1981 ccacgcggct ttaccactcc attttagagc cccatggctg gcgggacacg ggagaagcca
     2041 ttgcggccgc cttccgcaaa ggagattttt ccgccatggc ggacgcggtg agcgacgaga
     2101 tggtcgacgc catcgccatt gcggggacgc cggacgaggt gcgcgaccag atcacgcagt
     2161 gggagggtct tgccgaccac gtcattctct actcgcccag cgtggggatg cgcggcggtc
     2221 gggttcagga gaatctggat gccatcgtgg acacgtttgc agcttgaggt aaaacatggc
     2281 ggttgcgtcg aacatcagtt tcagtgagaa ggtggctcag gggttcatga gggcgccgtc
     2341 cggggccggg ggcggtttgc ccgagttcct cggcgtgaag atcaccgacg cgggtccggg
     2401 caccatgacc gggcagttcg aggtcaggcc ggaattgctc acgcccatcg gcaacatgca
     2461 cggcggcgtg ctatccgctt tctgcgatca catgctggga tgcgtctgct atccggccat
     2521 gaagccgggc cagtgggcgg ccaccaccga gttcaagatc aacctcacgg caccggtcac
     2581 ccagggcgtc gtggaggctc gtgcggagat cgtcaacatg tcgcggactc aggccgtggt
     2641 gcggctcgac ctgaccaacg aagggcgggt cgtggcgatg gctcagggca cggtgacggt
     2701 acgcgatccg cgctgaccag cgcctggcgc tgccatgctg ctaacatggc ggacggccgt
     2761 cgagctgttc gcgatgatcg aaagaggacc cgggcccatg atgattcgac acggttggct
     2821 gttgctcctg ctgctgatgg gttgctcgtc cacggtagtt ctcgaccggg acgtgccgtc
     2881 gggattcgac ctcgacggcg agtgggtggt ggatgcggcg gccagcgaag tcgcgcccgg
     2941 aacccggcgg ctgcgggcgc agggcatcgg catttccctg gtcgcccacg actttcccgt
     3001 gctgcgctgt gagcgcatga ccatcgagca gagcgcggat tccatgggca tcgagtacga
     3061 cggcgcgtcc taccgcgacg tctcctgggg tgtccgtcag cgcgggttgt gggaagtgaa
     3121 cgccggctgg gaggaagggg tattccgcat tctttccaag gcgcgggacg ccaaagcgga
     3181 ggagatgatg accctcgccg attccggccg ccggctcatc gtgcaagtgg tggtggagtc
     3241 ggaaggggac accatcgaca tcaccaggca attcgttcgc cgctagaaag ctccatc
//
LOCUS       DBNHKV010000322         3902 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1957200, whole genome shotgun sequence.
ACCESSION   DBNHKV010000322 DBNHKV010000000
VERSION     DBNHKV010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1957200"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="AC16PP_10135"
     CDS             complement(<1..442)
                     /locus_tag="AC16PP_10135"
                     /inference="COORDINATES: protein motif:HMM:NF017973.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482026.1"
                     /translation="MSEEEHPLEPFKRATTATIRAIAENDELEVTFGQGPPTVRGNRI
                     RVPLPAVGSAPEEVDAIRGMGDEFALRIRYHDDDVHRSRAPRSGPAQEMFQWIEDARI
                     ASIGSLRMEGVAQNLDASLEVQCQQAAFDTITAEADAPLSVAVGL"
     gene            complement(459..1460)
                     /gene="cobS"
                     /locus_tag="AC16PP_10140"
     CDS             complement(459..1460)
                     /gene="cobS"
                     /locus_tag="AC16PP_10140"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HIR6482027.1"
                     /translation="MTVNQSGLSADMAPDTTVSAREQFGVEHDFQVPAFSKRSDYVPE
                     VDVDYRFDPDTTLAILAGFAHNRRVMIAGYHGTGKSTHIEQVAARLNWPCIRVNLDSH
                     ISRIDLIGKDAIVLEEGKQVTEFREGILPWAIQHPVALVFDEYDAGRPDVMFVIQRVL
                     EVEGKLTLLDQNKVITPHPFFRLFSTTNTIGLGDTTGLYHGTQQINQAQMDRWSIVTN
                     LNYLEHDAEVDIVLGKAKHYAETEDGARTISNMVAVADLTRKGFVNGDVSIVMSPRTV
                     ITWAENAEIFGDIGYAFRVTFLNKCDELEQPIVAEYYQRCMGEDLGYATAGIALKSA"
     gene            1769..2332
                     /locus_tag="AC16PP_10145"
     CDS             1769..2332
                     /locus_tag="AC16PP_10145"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6482028.1"
                     /translation="MSELDELRTRVDRLESREAIRELVSAYAVACDHHDMPALDDLFT
                     EDAVFASPNGAMIATGRNAIHDMFVRILGIRGPGYHWTHDVTVNIDPSDPDRATGVVH
                     SHAETTPSGVMSIAAMRYHDSYRREAGIWRFASREISFLYYVPVSEYGASLTRPDRVV
                     MGTERHAADYPEALEAWQAFEASHGGA"
     gene            2319..3512
                     /locus_tag="AC16PP_10150"
     CDS             2319..3512
                     /locus_tag="AC16PP_10150"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6482029.1"
                     /translation="MAELEVLTHDEEMMSLARRALEHFVNKTTDQAASTLEMPVEAYV
                     GQSRYQAERDRIFMHLPLALALSLELPEPGCYRAMTVMNVPVLLIRGEDDTVRAFVNV
                     CTHRGARLCPEGAGERRVISCPYHAWTFDFEGRLSGRYAAETFGDLDPEAYALTELAC
                     AERAGVIWVILTPGEQFDADAWLGGFAEQLGSLHLTDWYLYEQRDLKGPGWKVAMDGY
                     LEIYHHNPLHGATVGQHTVGNLLVLDTYGPHQRLTLGRRTLGTLAETPESEWAPLEHI
                     RLIHSGFPNLSVSGILGDHCLVSQIFPGEDPFGTVTRQSILVAEKPATDAAEAAARNF
                     SDMVLQAVRDEDYAIGQTIQAGLRAPARAAFIFGRNEPAVQNYHRWVANFMTQTAEAR
                     DWGGV"
     gene            complement(3551..>3902)
                     /locus_tag="AC16PP_10155"
     CDS             complement(3551..>3902)
                     /locus_tag="AC16PP_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482030.1"
                     /translation="AVFAGDGARVSRILANLRQEGVSLYAVLAALTGQLRRLPGGRMA
                     PQRQRLVDGFMRRMRSPRNLDRALAQCALVDAQGKGQLLGDAWLSLENVCLRLAGQRG
                     LPSLEEELPSLRRA"
BASE COUNT          663 a         1293 c         1278 g          668 t
ORIGIN      
        1 tgagcccgac ggcgacgctc aagggggcgt cagcctcagc ggtgatggtg tcgaaggccg
       61 cctgctggca ctgcacttcc aggcttgcat ccaggttctg ggcgacaccc tccatacgca
      121 gggagccgat ggaggcgatg cgggcatcct cgatccactg gaacatttcc tgggccgggc
      181 ccgatcgcgg cgcgcggctg cgatgcacgt catcgtcgtg atagcggatg cgcagggcga
      241 actcgtcacc catgccgcgg atggcgtcca cctcctccgg tgcggacccc accgccggaa
      301 ggggcacccg gatccggttg ccgcgcacgg tgggcggccc ctggccgaag gtgacctcca
      361 gctcgtcgtt ctccgcgatg gcccggatgg tggctgtggt ggcccgcttg aacggctcta
      421 gcgggtgttc ttcctcagac atcggcgctt gtcaggactc aagccgactt gagcgcaatt
      481 cccgccgtgg cgtaacccag atcctcgccc atgcagcgct ggtagtactc ggccacgatg
      541 ggctgctcga gctcgtcgca cttgttcagg aacgtcacgc ggaaggcgta accgatgtct
      601 ccgaagatct ccgcattctc cgcccaggtg atcaccgtcc gcggggacat cacgatggag
      661 acgtcgccgt tgacgaagcc tttgcgggtg agatcggcca ccgccaccat gtttgagatc
      721 gtgcgcgcgc cgtcctccgt ctccgcgtag tgcttggcct tgcccagcac gatatccacc
      781 tccgcatcgt gctcgaggta gttgaggttg gtcacgatgc tccagcggtc catctgggcc
      841 tggttgatct gctgggtgcc gtggtacagc ccggtggtgt cgccaagacc gatggtgttg
      901 gtggtggaga agagacggaa gaacggatgg ggcgtgatga ccttgttctg gtcgagaagg
      961 gtgagcttgc cttcgacctc cagcacccgc tgaatgacga acatcacgtc ggggcggccc
     1021 gcgtcgtact cgtcgaatac cagcgccacc ggatgctgga tcgcccaggg caggatgcct
     1081 tcgcggaact cggtgacctg cttgccctcc tccagcacga tggcgtcctt gccgatcagg
     1141 tcgatccggc tgatgtggct gtcgaggttc acgcggatgc acggccagtt cagccgcgcg
     1201 gccacctgct cgatgtgggt ggacttaccc gtgccgtggt agccggcaat catcacccgg
     1261 cggttgtggg cgaaaccagc gaggatcgca agcgtggtgt ccggatcgaa acggtaatcc
     1321 acgtccacct ccggcacgta gtccgatcgc ttgctgaacg ccggcacctg gaagtcgtgc
     1381 tcgacgccga actgttcccg ggcactaacc gtcgtgtccg gagccatgtc cgcggacagg
     1441 ccggattggt ttaccgtcac tgcaattcct caactgcggg cgagccgttt attgtaagga
     1501 accaccggta aaagagcagc agatgcgggc gattaatctc gctgcccgcc aggccggcgt
     1561 tttcgcacca cctgggcaaa gagttggtca aatccgcgag gacgcccggg cggcagaccg
     1621 gctcaagccc gctcgaacgc ccgatttccg ccgtctgcag gccctctcca gccgattggg
     1681 cgcgggccgg ctgcgggcct tgttgatcgc gtggtgagcg ccctctatat tcagttgaat
     1741 caagcgatga agagctgggg aggcgatctt gagcgagctg gacgaattgc gcacacgagt
     1801 ggaccgtctg gagtccagag aagccatccg cgagctggtg tcggcctacg ccgttgcctg
     1861 cgaccaccac gacatgccgg cgctggacga tctgttcacc gaggatgccg tgttcgcctc
     1921 accgaacggc gccatgatcg ccacgggccg gaacgccatc cacgacatgt tcgttcggat
     1981 tctcggtatc cggggcccgg gttaccactg gacccacgac gtgaccgtca acatcgatcc
     2041 gagcgacccc gaccgcgcca ccggcgtggt gcacagccac gcggagacca cgcccagcgg
     2101 cgtgatgagc attgcggcca tgcgctatca cgacagctat cgcagagaag ccggcatctg
     2161 gcgtttcgcc agccgcgaga tcagctttct gtactacgtg cccgtttccg agtacggggc
     2221 cagcctgacg cgccccgacc gcgtggtcat gggcaccgag cgacacgccg ccgactaccc
     2281 cgaggcgctg gaggcatggc aggcgttcga ggcgagtcat ggcggagctt gaagtcttga
     2341 cccacgatga agagatgatg tcgctggcgc gccgcgcgct cgagcacttc gtgaacaaga
     2401 cgaccgacca ggcggcatcc accctcgaga tgccggtgga ggcctatgtg gggcagtcgc
     2461 gctaccaggc agagcgggac cgcatcttca tgcatctccc gctggcgctg gcattgagcc
     2521 tcgagctgcc cgagcccggc tgctaccggg ccatgacggt catgaacgta cccgtcctgc
     2581 tcatccgcgg ggaagacgac accgttcgtg cttttgtcaa cgtctgcact caccgggggg
     2641 cacggctgtg cccggaaggt gccggagagc gcagggtcat cagctgtccc taccacgcct
     2701 ggactttcga cttcgagggc cgactgtcgg gccggtacgc ggcggagacc ttcggcgacc
     2761 tggaccccga ggcttacgcg ctcacggagc ttgcctgcgc cgagcgggcc ggcgtcatct
     2821 gggtgatact caccccgggc gagcagttcg acgcggacgc ctggctcggc gggtttgccg
     2881 agcagctggg ttcgctgcat ctcacggatt ggtatctgta cgagcagcgg gatctcaaag
     2941 gcccgggctg gaaagtcgcc atggacggct acctggagat ctatcatcac aacccgctgc
     3001 acggtgccac cgtggggcaa cacaccgtgg gaaacctgct ggtgttggat acctacggcc
     3061 cccaccagcg gctcaccctg gggcgccgca cgctggggac gctggcggaa acgccggagt
     3121 ccgagtgggc tccgctcgag cacattcgcc tgatccactc cgggtttccc aacctgtctg
     3181 tctcaggaat cctcggcgac cactgcctgg tcagccagat cttcccggga gaggacccgt
     3241 tcggcaccgt cacccggcag agcatcctgg tggcggagaa accggcaacg gacgccgccg
     3301 aggccgctgc ccgcaatttc tccgacatgg tgctccaggc ggtccgcgac gaggactacg
     3361 ccatcggcca gaccattcag gccgggcttc gtgcccctgc gcgcgccgcc ttcatcttcg
     3421 gccgcaacga gcccgcggtg cagaactacc accgctgggt ggccaacttc atgacccaga
     3481 ccgccgaggc gcgggactgg ggcggggtgt gagccgggcc tgctgatcgc ccgcttggcg
     3541 ccgggcacga tcaggctctg cgcagcgaag gcagctcctc ctcgagagac ggcaggccgc
     3601 gctgtcccgc cagccgcaga cagacgttct ccagagaaag ccaggcatcc ccgagcagct
     3661 ggcccttacc ctgagcgtcc acgagcgcgc actgggccag ggccctgtcg aggttgcgcg
     3721 gggacctcat gcgacgcatg aagccgtcga ccaggcgctg gcgctggggc gccatccgcc
     3781 cgccgggcag acgccggagc tgaccagtca gtgcagccag aacggcgtag agggaaacgc
     3841 cctcctgccg gagattggcc agtatgcggc tcacccttgc cccatcgccc gcgaaaaccg
     3901 ca
//
LOCUS       DBNHKV010000323         4797 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_541712, whole genome shotgun sequence.
ACCESSION   DBNHKV010000323 DBNHKV010000000
VERSION     DBNHKV010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_541712"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="AC16PP_10160"
     CDS             <1..550
                     /locus_tag="AC16PP_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482031.1"
                     /translation="PYDYILGFISSSLNDMATAVQGYYASPEGRALEAKANETSDCIS
                     AIVMSEQLRNGVIGNTGDDQLDAVVELFSCTLNKGSDLDDVRSDASFLNKQIDAIGSA
                     ALSQYEAYLWSPYRGGTGSWDFLWVGNYPNLSTWAQGDTDYYASKEGMAAEERFQKTS
                     TCTNSIWGGYWIVPPAEGPTAQ"
     gene            743..2113
                     /locus_tag="AC16PP_10165"
     CDS             743..2113
                     /locus_tag="AC16PP_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6482032.1"
                     /translation="MTQPRLSKATLFCYGVADLPVMLAIMPMSIWLSRFYTGDMGLSL
                     SAVANIMLLARLFDVFTDPLVGHLSDHTRTRWGRRKPWMLASLPLLMIGLYKVFLPPD
                     GIGIWYLFSWMMVMWLGWTMLMIPYYAWAAELSDDYDERTRITGWRAVMGSVGGIGAQ
                     LIPFIALVLFGFGGTANVMTMLGIAALILMPLCVGVALLRVPEFPEMRAPSVPILAGI
                     RIMWRNGPFRRLLLAFVLSSTGLAIVMPLYIFFVEFIVEEPPANVPYMIIISSVAGFL
                     GIPFWVWLSKHVGKHKAWIGGFLVVAAVSPMYLFLGPGDFWLMVPGIIIIGIGTGSFS
                     ALPNSMKADVIDLDTARSGKNRAAFFFSAWSLVTKLASSLGGWLALQSLALFGFDAAN
                     GAQNTPEALTGLRLTFAVLPAIIFVIAAAVIWRYPITKERQARIRAAIDRRTLRRNAA
                     QAGFAK"
     gene            2121..3326
                     /locus_tag="AC16PP_10170"
     CDS             2121..3326
                     /locus_tag="AC16PP_10170"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HIR6482033.1"
                     /translation="MIARTGVLLVSMLWAASAKAEPVGFAACVSNLESVAVASGISEA
                     TAKQVLGEVRHLERVIELDRTQPEFVRTFSEYYRVRVTPSRVADGRRLLEENAALLGR
                     LQRQYGIPGHYLIALWGLETNFGSYFGNMSTMDSLATLACDERRSAFFTAELMDALRI
                     VDAGDMEPAAMQGSWAGAMGHVQFMPSVFRRYAVDDDGDGRRDLWTSRRDALASAANY
                     LKQAGWERGFRWGREVRLPEAFDYAGAARDPTRPLADWEAAGVRNAFGGPLASTDLPA
                     TLRLPSGHRGPAFLTYPNFEVIMQWNRSESYALSVGNLADRIAGRSPLTRPPPTDELK
                     LTPDSVRQLQADLNALGLDAGEADGVIGPATRAAIGRFQIGHGLVPDGYPDHRLVEAL
                     RASREAGER"
     gene            complement(3356..4267)
                     /locus_tag="AC16PP_10175"
     CDS             complement(3356..4267)
                     /locus_tag="AC16PP_10175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6482034.1"
                     /translation="MKIGLMMGATGTTTLDDVVALAKSAEAAGLDSLWMANIFSFDAI
                     STLAIIGRETSTIGLGTAVTPTYPRHPTAIAQQALTTAAASGSRFTLGIGLSHKIVIE
                     NMLGFSYDRPARHMREYLSVLMPLVRGETCNFQGEQYRVAGVRIDIPGEITMPVVVAA
                     LGPAMLKITGELADGTNTWMVGPKTMAEHIIPRISAAADEAGRPPPRIVGGYPVILTN
                     KPDEAREKIARELTIYGQLPSYRAMLDREGVAGPADIAIAGDENLVRGELKRLEDMGV
                     TDFNAAIMPVEEGAQERTVSFLASLKG"
     gene            4372..>4797
                     /locus_tag="AC16PP_10180"
     CDS             4372..>4797
                     /locus_tag="AC16PP_10180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6482035.1"
                     /translation="MDVASFTDLSEKFTEITERIVWCTVSTVDAKGRPRSRILHPVWE
                     GATGWIATGRHSHKARHLANNPYVSLSYWDPQHEQTMIECKAEWQDDQPTRDRIWELL
                     KTTPEPVGYDPALFWSSAADENFGVLKLTPWRLEVWSLAA"
BASE COUNT          786 a         1606 c         1566 g          839 t
ORIGIN      
        1 gccatacgac tacattctcg gattcatcag ctccagcctg aacgacatgg ctaccgccgt
       61 gcaggggtac tacgcgtcgc ccgagggtcg ggcattggaa gccaaggcca atgaaacgtc
      121 agactgcatc tccgccatcg tcatgagcga gcagcttcgc aacggggtca tcgggaatac
      181 aggcgacgac cagctggacg ccgtggtcga gctgttctcc tgcactctga acaaaggatc
      241 cgatctcgat gacgtgcgca gcgacgccag ctttttgaac aagcagatcg acgccatcgg
      301 ctccgccgcg ctgagtcagt acgaggcgta cctctggagt ccgtatcgag gcgggaccgg
      361 cagctgggat ttcctctggg tcggcaacta tcccaacctc tcgacctggg cccagggaga
      421 caccgattac tatgcctcta aggagggcat ggcggccgag gaacggttcc agaagaccag
      481 cacctgcacc aactccatct ggggcggcta ctggatcgta ccgcccgccg agggacccac
      541 cgcccaatag ggtactccac catgccggcc ctggaagcac accagggtcg gcatgacgcc
      601 gtcgccggct tgggaaccgt cacgacggga ttgacgacca gcggccgatc gccgagcacc
      661 cagagcgcgc cgagtcatgg tggattcctc aggcgcggcc gacttacact gaagcctcca
      721 agcccgccaa tcgggaacgc agttgaccca gccgcggctc agcaaggcca ccctgttctg
      781 ctacggcgtc gccgatcttc cggtgatgct ggcgatcatg cccatgtcca tctggctgtc
      841 gcgcttctac accggcgaca tgggcctgag cctgtcggcg gtggccaaca tcatgctgct
      901 ggccaggctc ttcgacgtgt tcaccgaccc cctggtgggg cacttgagcg atcacacccg
      961 cacccgatgg ggcaggcgca agccctggat gctggcgtcg ctgccattgc tcatgatcgg
     1021 cctgtacaag gtgtttctgc cgcccgacgg catcggcatc tggtacctgt tctcgtggat
     1081 gatggtcatg tggctcggct ggaccatgct gatgattccc tactacgcct gggccgccga
     1141 gctctccgac gactacgacg agcgcacgcg catcaccggc tggcgcgcgg tcatgggcag
     1201 cgtgggaggc atcggcgccc agctgatccc cttcatcgcg ctggttctgt tcggcttcgg
     1261 cggcaccgcc aacgtcatga ccatgctagg tatcgcggcg ctcattctca tgcccctgtg
     1321 cgtgggcgtg gcgctgcttc gggtgcccga gtttccggag atgcgggcgc cgtcggtgcc
     1381 catcttggcg ggcatccgga tcatgtggcg caacggtccg ttccgtcgcc tgttactcgc
     1441 tttcgtcctg tcctccaccg ggctcgccat cgtcatgccg ctgtacatct tcttcgtcga
     1501 gttcatcgtc gaggagccgc ctgccaacgt cccctacatg atcatcatca gctcggtagc
     1561 cggttttctg ggcataccgt tctgggtctg gctctccaag cacgtaggga agcacaaagc
     1621 gtggattggc ggctttctgg tggtagccgc ggtgagcccc atgtatctct tcctcggccc
     1681 cggcgacttc tggctgatgg tgcccggcat catcatcatc ggtatcggca ccgggtcctt
     1741 cagcgccctg cccaactcca tgaaggccga cgtgatcgac ctggacacgg cacgcagcgg
     1801 caagaaccgg gccgccttct tcttctccgc gtggtccctg gtgaccaagc tggcctcgag
     1861 ccttggcggc tggctggctc tgcagtctct ggccctgttc ggcttcgacg cggccaacgg
     1921 tgcgcagaac acgccggagg cgctgaccgg gctgcggctc accttcgccg tgctgccggc
     1981 catcatcttc gtgatagctg ccgccgtgat ctggcgctac ccgatcacca aggagcgcca
     2041 ggcccgcatc cgggcagcca tcgaccggcg cacgctgcgc cgaaacgcag cccaagcagg
     2101 cttcgcgaag taacgccgcg gtgatcgcgc gaacgggcgt gctgctcgta tccatgctct
     2161 gggccgcgtc ggctaaagcc gagcccgtgg ggttcgcggc ctgcgtcagc aacctggaat
     2221 ccgttgccgt ggcgtccggg atttccgagg cgaccgcgaa gcaggtactc ggcgaggtac
     2281 gccacctgga gcgggtgatc gagctcgacc gcacgcagcc ggagttcgtt cgcacgttca
     2341 gcgagtacta ccgggtgcgg gtgaccccat cgagggtggc cgacgggcgc cgcctgctcg
     2401 aggaaaatgc cgcccttctc ggccggctgc agcgccagta cgggattccc ggccactacc
     2461 tgatcgcgct gtggggtctg gagaccaact tcggcagcta cttcggcaac atgagcacca
     2521 tggactcgct cgccacgctg gcctgcgacg agcgccgcag cgccttcttc acggcggagc
     2581 tgatggacgc cctgcgcatc gtcgacgccg gggacatgga gcccgcagcc atgcagggct
     2641 cctgggcggg cgccatgggc cacgttcagt tcatgccttc ggtcttccgc cgctacgccg
     2701 tggacgacga cggcgacggc agaagggatc tctggaccag ccggcgcgac gccctggcat
     2761 ctgcggccaa ttacctcaaa caggctggct gggagcgggg tttccgctgg gggcgcgagg
     2821 tacgcctgcc cgaggcgttc gactacgcgg gcgccgcccg tgaccccacg cggcccctcg
     2881 cggattggga ggccgccggg gtgcgcaacg cgttcggagg cccgctggcg tccacggatc
     2941 tgccggccac cctgcgcctg ccctccgggc accgggggcc tgcgtttctg acgtatccca
     3001 acttcgaggt gatcatgcag tggaatcggt cggagagcta cgcgctgagc gtcggcaacc
     3061 tggccgaccg gatcgccgga cggtcaccgc tcaccaggcc gccacccacg gacgagctga
     3121 agctgacgcc ggactcggtg cgccagctgc aggcggacct gaacgccctg gggttagatg
     3181 ccggcgaagc cgacggcgtg atcggcccgg cgacccgggc cgccatcggg cggttccaga
     3241 tcggtcacgg tctggtcccg gacgggtatc ccgaccatcg gctcgtggag gccctgcgcg
     3301 ccagccggga agccggcgag cgctgaacct caaaagggat cgctgaagcc gggactcagc
     3361 ccttgaggct ggccaggaac gacaccgtgc gctcctgcgc cccttcctcc accggcatga
     3421 tcgcggcgtt gaaatccgtc acccccatgt cctcgagccg cttgagctcg ccgcgcacca
     3481 ggttttcgtc tccggcaatg gcgatgtccg cggggcccgc caccccctcc cggtcgagca
     3541 tggcgcggta gctgggaagc tggccgtaga tggtgagttc ccgggcgatc ttctcccgtg
     3601 cttcgtcggg cttgttggtc aggatcaccg ggtaaccgcc cacgatgcgc ggtggcggac
     3661 gcccggcctc gtccgcagcg gcgctgatcc gggggatgat gtgctcggcc atggtcttcg
     3721 ggcccaccat ccaggtgttg gtgccgtcgg ccagctcacc ggtaatcttg agcatcgccg
     3781 gcccgagagc ggcgaccacc acgggcatcg tgatctcgcc ggggatgtcg atccgcacgc
     3841 cggcaacccg gtactgctcg ccctgaaaat tgcaggtctc cccccgaacg agcggcatca
     3901 gcacgctgag atattcacgc atgtgtcgcg cgggtcggtc gtaggagaag ccgagcatgt
     3961 tctcgatcac gatcttgtga gaaagcccga tacccagggt aaaccggctg ccgctggcgg
     4021 ccgccgtggt cagggcctgc tgggcgatgg ccgtggggtg cctcgggtag gtgggcgtaa
     4081 ccgcggtgcc aagacctatg gtgctggtct cgcggccgat aatggccagg gtggatatgg
     4141 catcgaagga gaagatgttc gccatccaga ggctgtcgag cccggccgct tcagcgctct
     4201 tcgccaaagc gacgacgtcg tcgagcgtgg tggtgccggt ggcgcccatc atgagtccta
     4261 ttttcatttc tgccccctag gcgatggttg tacaggtcgg gaaatgccgc acaataaggc
     4321 aaaccagggc cggcaacaaa gccgcctgga ggcgaacggg gaggccggcg agtggacgtg
     4381 gcgtcattta cggatctcag cgagaagttc acagagatca cggagcgcat cgtgtggtgc
     4441 acggtcagca cggtggacgc caagggccgg ccccgctcgc gcatcctcca tccggtctgg
     4501 gagggcgcca cgggctggat tgccacgggc cgccactcgc acaaggcccg gcacctcgcg
     4561 aacaacccct acgtctcgct gagctactgg gatcctcagc acgagcagac gatgatcgaa
     4621 tgcaaagccg agtggcagga cgaccagccc acccgggacc ggatctggga actgctgaag
     4681 accacgccgg agcccgtggg ctacgacccc gcgctgttct ggtcaagcgc tgcggacgag
     4741 aatttcggtg tcctcaagct cacaccctgg cggctcgagg tctggtccct ggccgcc
//
LOCUS       DBNHKV010000324         2854 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1250499, whole genome shotgun sequence.
ACCESSION   DBNHKV010000324 DBNHKV010000000
VERSION     DBNHKV010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1250499"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="AC16PP_10185"
     CDS             <1..461
                     /locus_tag="AC16PP_10185"
                     /inference="COORDINATES: protein motif:HMM:NF052145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA-type ATPase lid domain-containing protein"
                     /protein_id="HIR6482036.1"
                     /translation="VELHVPPLRERQDDILPLARVLLADAAVRMARKISGLTPRTADQ
                     LLRYDWPGNVRELENTMERAVALARGSRVDLDDLPEEIRQAFPTPVAGGAAVQPLRDV
                     EKDYILAVLKLNGGNQTRTAEQLRIGAATLYRKLKKWGLIGAEGAAQARG"
     gene            complement(468..815)
                     /locus_tag="AC16PP_10190"
     CDS             complement(468..815)
                     /locus_tag="AC16PP_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482037.1"
                     /translation="MQDTHLMTEMIEKCRRVILDQDEHALEHQPRPLRKTHLIRMCDR
                     MIDHVDDWPAVKLNRWIGFIQCALIANRSLDLEEAKQMFNRAKKTSGESAQDLLDHLD
                     PYSPFEFELGGEG"
     gene            complement(1009..1974)
                     /locus_tag="AC16PP_10195"
     CDS             complement(1009..1974)
                     /locus_tag="AC16PP_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR6482038.1"
                     /translation="MPFDSMLNILTLGVLVIGAITVLRATLFTVEQRTTAIVQRFGRF
                     VREVGPGIRARIPFVDRVVGRVNLRVQQLDVEIETKTEDNVFVRMVVAVQYYVLPEKV
                     YDAFYKLEDANRQITSFVFDVVRAQVPKIKLDDVFEKKDDIANIVKSELAQVMEGFGY
                     GILKALVTDIDPDPKVKEAMNEINAAQRMRVAATERGEADRILKVKAAEGEAQSKALQ
                     GRGIADQRQAIVAGLRDSVDEFRKSVPGTTAKDVMNLVLMTQYFDMLKEIGASSRSNA
                     ILIPHSPGSLASLTEQMRNAMIEADQTVRGAEMPRGVELKEPVLV"
     gene            2257..>2854
                     /locus_tag="AC16PP_10200"
     CDS             2257..>2854
                     /locus_tag="AC16PP_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017528268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR6482039.1"
                     /translation="MSGYLSHTAGKETYVLEDRCLGEGHAACHLLGRTREEWGDEHAA
                     ELAFFDSGRLTECLDVSLSRVTKTLKAAEEKLRAHRRALVRVVRDVEQPLGIVAKSPN
                     MQNVVDLARRVAKVDATVLITGESGVGKERIARLVHDESTRAAGPFIAVNCGAITETL
                     LESELFGHTRGAFTGAASDRPGLFEAANRGTLLLDEIGD"
BASE COUNT          504 a          918 c          861 g          571 t
ORIGIN      
        1 tggtcgagtt gcacgtccca ccgctgcgtg aacgacagga cgacatcttg ccgctcgccc
       61 gggtgctgct cgccgacgct gcggtccgga tggctcgcaa gatctcgggc ctcacaccac
      121 ggaccgccga tcagctcctg cgctatgact ggccgggcaa cgtgcgcgaa ctcgagaaca
      181 ccatggagcg cgccgtcgcc ctggcgcgcg gaagccgcgt ggatctcgac gatctgcccg
      241 aggagatccg ccaggcgttt cccacaccgg tggccggcgg cgcagcggtg cagccgctcc
      301 gtgatgtcga gaaggactac atcctggcgg tgctgaagct caacggcgga aaccagacgc
      361 gcaccgccga gcagctccgg atcggcgcgg cgacgcttta ccggaagctc aagaagtggg
      421 gcctgatcgg cgccgagggc gcggcacaag cacggggctg agcggcctca accctcgcct
      481 ccgagctcga actcgaacgg gctgtacggg tccagatggt ccagaaggtc ctgggcactt
      541 tcgccagagg tcttcttcgc ccggttgaac atctgtttcg cttcctcgag atcaaggctg
      601 cgattggcaa tcaatgcgca ttgaatgaac ccgatccagc ggttcagctt gacagccggc
      661 cagtcatcga catgatcgat catccggtca cacatgcgga tcaggtgcgt ctttctgagc
      721 ggcctcggct ggtgctcgag ggcatgctca tcctgatcca ggatgaccct cctgcacttt
      781 tcgatcatct ccgtcatcag atgcgtgtct tgcatggcgt cactcctcga cccttggagt
      841 ttgaggaccc tgctccctca gggtccatcc ggcttctact cttcgttgga gtacaagagc
      901 tgaatcagta catgccgtcg cccatgccgc ccagtggttt tgggtgctgg tgtggcgttg
      961 gtggacattt cgggcctccc cttggctcct ttcgctacat cccagcgctc agacgaggac
     1021 gggctccttc agctctaccc ctcggggcat ctcggccccc cggaccgtct gatcggcctc
     1081 gatcatggcg tttcgcatct gctctgtcag actggcgagg ctgcccggag aatgcgggat
     1141 gagaatcgcg ttgctgcgag acgaggctcc gatctctttg agcatgtcaa agtactgggt
     1201 catgaggacg aggttcatca cgtccttcgc ggtcgtaccc ggcaccgact tgcgaaactc
     1261 gtccaccgag tcccgtagac ccgcgacgat ggcctgcctt tgatcggcga tgccacggcc
     1321 ttgcagggcc ttgctctgag cctcgccttc ggccgccttg accttcagga tccgatcggc
     1381 ttcgcctctt tcggtcgccg ccactcgcat ccgctgcgcc gcgttgatct cgttcatcgc
     1441 ctccttcacc ttggggtcgg gatcgatgtc ggtcaccagc gccttgagaa tcccatatcc
     1501 aaagccttcc atgacttggg ccagctcgct cttcacgatg ttggcgatat cgtccttctt
     1561 ctcgaacacg tcgtcgagct tgatcttggg aacctgggcc cggaccacgt cgaagacgaa
     1621 cgaggtgatc tgacggttcg cgtcttcgag cttgtagaac gcgtcgtaga ccttctccgg
     1681 aagcacgtag tactgcacgg cgacgaccat gcgaacgaac acgttgtcct ccgtcttggt
     1741 ctcgatctcg acgtccagct gctgaacacg caggttgacc cggccaacca cccgatcgac
     1801 gaagggaatc ctggctcgga tgccggggcc cacctcccgc acgaatcttc cgaagcgctg
     1861 gacgatcgct gtcgtgcgct gctcgaccgt gaataaggtg gcgcggagca cggtaatcgc
     1921 tccgatcacc agcaccccaa gagtgagaat gttgagcatg gaatcaaagg gcatcgtcat
     1981 ttcctttccg tcggttcgac gttgcgccac ccggaactcg accgagacgt cgatcgcttg
     2041 gcggccttgc ttgattccaa ctgcaagtct ccggccacag tcctatctca tttgtttgta
     2101 gctgtttgtg cccgcataga cggtttagcc cgctgtgcga tcaatcgata tgaagtaggc
     2161 tgtacctcaa tctgaaggac acgtcgatgc gggtcgaaca gcatctcctg cttcggccgc
     2221 tccgactcgg gcgtgtgctg gaccatttgc ggcctcatga gcggctacct cagccacacc
     2281 gccggcaagg aaacctacgt cctcgaagat cgctgtctgg gcgaaggtca cgcggcgtgc
     2341 cacctgctgg ggcgcacccg cgaagagtgg ggtgacgaac acgcagcgga gcttgccttc
     2401 ttcgactcgg gacgcctcac cgagtgcctc gatgtctcgc tcagcagggt caccaagacc
     2461 ttgaaggctg ccgaggagaa gctccgagcc caccgtcgag ccttggtgcg cgtggtacgc
     2521 gacgtggaac agccgctggg catcgtggcc aagagcccca acatgcagaa cgtggtggac
     2581 ctggcacgac gcgtggcgaa ggtcgacgcc acggtgctca ttaccggcga gagcggagtg
     2641 ggcaaggaac gaatcgcccg actcgttcac gacgagtcga cgcgagcggc gggacccttc
     2701 atcgccgtca actgcggcgc catcaccgag acgctcctcg agagcgagct tttcgggcac
     2761 acgcgcggtg cgttcaccgg cgcggcttcg gaccgacccg gcctgttcga agcagccaac
     2821 cgcgggacgt tgctgttgga cgagattggt gacg
//
LOCUS       DBNHKV010000325         1670 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_542409, whole genome shotgun sequence.
ACCESSION   DBNHKV010000325 DBNHKV010000000
VERSION     DBNHKV010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_542409"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..>1670)
                     /locus_tag="AC16PP_10205"
     CDS             complement(244..>1670)
                     /locus_tag="AC16PP_10205"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6482040.1"
                     /translation="LMEGVEDIPGTALYEGIEWGNWESFPEYIEYLSTKRYTMDIGTQ
                     IPHSALRNYVMGERALTHEDATAEDLEAMCRLVQDGLAAGALGFSTSRTIGHRSVDGE
                     PIPGTFAEKDELLAIARAMREAGRGVFQAVPAGVVGDIAGPERWTTEQEVELFAEVAR
                     ASGRKCTFTLIQNGNRPDQWRNCLNIVEQANAEGIELTPQIATRPIGIVTSLDSYHMF
                     MRRETYLKYADLPLSSRLAELRKPEVRAAILADRDVPPDQPGVMANVFALMQMAAPAM
                     FPIDIPIDYEPEPSANMGARAAAAGMDIQAFMYDFLTAADGDQFAILLGANFADGTFS
                     VIEEMIPHPNTTIGLSDVGAHVNLIFDAVGPTYQLTHWVRDRSRGRRFPIELMVHKQT
                     YSNARLFGLNDRGSIEVGKRADINVIDLERLGLGKLHVHRDLPAGGSRILQPAHGYVG
                     TFVAGVKTRENDRDTGARPGRVIR"
BASE COUNT          262 a          524 c          560 g          324 t
ORIGIN      
        1 ctttgcaggg tctgcgcgat gcggctgatc ggggtcgcgc cctcgggatg gcaggcaggg
       61 aaattgtgga ggcgacatac gactgccgac gcaacgcaaa gcggatagcg gccttgttgc
      121 gtgctgccgt tgctcaatga atccggtatc gagccgcttt gcgggtctca acccgggttt
      181 tttcggtcgc acgcctgctc tattggcagg accagcagct ccggaagacc accgctagcg
      241 tagctatcgg atcacccggc caggccgcgc gccggtgtcg cgatcgtttt cccgggtttt
      301 cactccagcc acgaacgtgc cgacgtagcc gtgggcgggc tgcaggatgc ggctgccgcc
      361 ggccggcaga tctcgatgca cgtgcagctt gccgaggccc aaccgctcca ggtcgatgac
      421 gttgatgtcg gcccgcttgc cgacctcgat gctgccccgg tcgttcaggc cgaacagcct
      481 ggcattgctg taggtctgct tgtgcaccat cagctcgatg ggaaaccgtc tgccccggct
      541 gcggtcgcgc acccagtgtg taagctggta ggtgggtccc acggcgtcga agatgaggtt
      601 gacgtgcgca cccacatcgg aaaggccgat ggtcgtgttg gggtggggaa tcatctcctc
      661 gatcacgctg aaggtaccgt cggcgaagtt cgcgccgagc agaatggcga actggtcgcc
      721 gtctgcggcg gtgagaaaat cgtacatgaa ggcttggatg tccatgcccg cagcggccgc
      781 acgcgcgccc atgtttgccg acggctccgg ctcgtagtcg atggggatgt cgatgggaaa
      841 catggctggt gcggccatct gcatcagggc gaagacgttg gccatcacgc ccggctggtc
      901 cggcgggacg tccctgtctg ccaggatcgc ggccctcacc tcaggctttc tcagctccgc
      961 gagccgcgaa gacaggggca ggtccgcata cttcagatag gtctcccggc gcatgaacat
     1021 gtgataggag tccaggctcg tgacgatgcc gatggggcgt gtcgcgattt gaggcgtgag
     1081 ctcgatgccc tcggcgttgg cctgctccac gatgttcagg cagttgcgcc actgatcggg
     1141 ccggttgccg ttctgaatca gggtgaacgt gcacttgcgg ccgctggctc gggccacctc
     1201 ggcgaaaagc tcgacttcct gctccgtcgt ccatcgctcc ggccctgcga tgtcgccgac
     1261 gaccccggcc ggcacggcct ggaagacgcc gcgtccggcc tccctcatgg cccgggcgat
     1321 ggccagcagc tcgtctttct cggcgaacgt cccggggatg ggctcgccgt caacggaccg
     1381 gtggccgatg gttctcgagg tagagaatcc cagggctccg gcggcgagac cgtcctggac
     1441 cagccggcac atggcttcga ggtcctcggc ggtggcatcc tcgtgggtga gtgcccgctc
     1501 gcccatcacg tagttacgca gagcgctgtg cggaatctgg gtgccgatat ccatggtgta
     1561 ccgcttggtc gaaaggtact cgatgtactc ggggaacgat tcccagttgc cccactcgat
     1621 gccctcgtac agtgcggtgc cggggatgtc ctcgaccccc tccatcagct
//
LOCUS       DBNHKV010000326         2163 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_471893, whole genome shotgun sequence.
ACCESSION   DBNHKV010000326 DBNHKV010000000
VERSION     DBNHKV010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_471893"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="AC16PP_10210"
     CDS             complement(<1..295)
                     /locus_tag="AC16PP_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482041.1"
                     /translation="MRADGAGCKRRDVGTAGRGGEDRGMNAIGAGREGTATADREGEA
                     VVLCCPGVGPAIARAGARGDPADLCALNAPPLAALLAPRAERARAIACCASMGE"
     gene            479..>2163
                     /locus_tag="AC16PP_10215"
     CDS             479..>2163
                     /locus_tag="AC16PP_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003282385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR6482042.1"
                     /translation="MKKAALPENEGERLERLRDYDILDTLPEQDYDDITYLASQICDT
                     PIAVISLVDDDRQWFKSAQGLDVSETPRDVAFCAHTILEPDKLMVVEDASQDDRFADN
                     PLVAGDLGIRFYAGAPLVTASGHALGTLCVIDQKPRGLTDQQRKTLAVLARQVVALLE
                     LRRSVAELKRSSDALHESHQFLALRGQQLNRSRDELAEAVRVLEGQADVIEQDLHRAE
                     VIQRSLLPHEVPRLEDFSVRSLYRPGHTVGGDLYDVVSIADRYLAIVVADACGHGVSA
                     AMLSVLFKQHLRLQDPASGIPYPPAWALERINRSVVANRPAPGVFLTAVYALVDTQTR
                     KLKLGSAGHPPVICLRASGEVESFAHTGPALGLDESSTYEEAEIELGVGDKLLFYTDG
                     LLDICESSPTVEALGQAMVSVADKPNLLEQVLMEVTHGRARPDCDDVTMVLLDAAPGE
                     SVFNESVEGLELQEMPTTASPTISWAASEDGVFLILEGWVTWLCGETLFNAAMPLIKA
                     HRRLVIDLELCEHMDSTMFGTLHELIQEADRLGSAVVLQHVGPRQMEDFEELS"
BASE COUNT          338 a          719 c          715 g          391 t
ORIGIN      
        1 gctcacccat tgaggcgcag caggcgatcg cgcgtgcccg ttcggctctc ggcgccagaa
       61 gcgccgccag gggcggggcg ttgagagcgc agaggtcggc cggatctccc cgcgctccgg
      121 cccgggctat ggccgggccg acaccggggc agcagagcac gacggcctcc ccttcgcgat
      181 ccgcggtagc tgtgccctcg cggccggctc cgatggcgtt catgcctcga tcttcgccgc
      241 cgcggccggc ggttcccacg tcccggcgct tgcagcctgc cccgtcggcg cgcacctccc
      301 ggccttctca gcccgctccg tcggcccgca cgtcccggcc ggccttcgat cccgggacca
      361 tttccaggcg gggcggcggg gttcgcgccg gcaggccgac gcccatgcgg ctcgactgag
      421 cgcgccagat agcgtttgcg gcttcgcctg gcagacttaa aggcgtaatc tgtacgtcat
      481 gaagaaggct gcgcttcctg agaacgaggg cgaacgtctt gagcggctgc gtgactacga
      541 tattctcgac acgcttcccg agcaggacta cgacgacatc acctatctcg cttcgcagat
      601 ctgcgatacc cccatagccg tcatcagcct ggtggatgac gatcgtcagt ggttcaagtc
      661 ggcccagggc ttggatgtct ccgagacacc gagagacgtt gcgttctgcg cacacacgat
      721 tctcgagccc gacaaactca tggtggtgga ggacgccagc caggatgacc gctttgcgga
      781 caaccctctg gtcgccggcg atcttggcat acgcttctat gccggcgccc ccctcgtcac
      841 cgcgtcgggt cacgccctgg gcacgttgtg cgtcatcgac cagaaacccc ggggactgac
      901 cgatcagcag cgcaaaacgc tcgcggtgct cgctcgccag gtggtcgcgc tcctcgaatt
      961 gcggcgttcc gtcgccgagc ttaagcgtag ctcggatgcc cttcacgagt ctcaccagtt
     1021 tctcgccctt cgggggcagc agctcaacag gtcgcgggac gagcttgctg aagccgtgcg
     1081 ggtgctggag ggtcaggcgg acgtgatcga gcaggatctg catcgggcag aagtgatcca
     1141 gcgttcgctg ctcccccacg aggtcccccg gctcgaggac ttcagcgtgc gatcgcttta
     1201 tcgcccgggg cacacggtgg gcggggatct gtacgacgtg gtgtcaatcg cggaccgtta
     1261 tctcgccatc gtggtcgccg acgcctgcgg tcacggtgtc agcgccgcga tgctctcggt
     1321 ccttttcaag cagcacctgc ggctgcagga cccggcgtcg ggcattcctt acccgccggc
     1381 gtgggcgctg gagcgcatca accgttccgt ggtcgccaac cggcctgcgc ccggcgtgtt
     1441 tctgaccgcc gtctacgcgc ttgtggatac tcagacccga aagctcaagc tgggctccgc
     1501 cggtcatccg ccggtaattt gtctgcgcgc gagcggcgag gtcgagtcgt ttgcccacac
     1561 gggcccggcg ttggggctcg acgagagttc gacttacgag gaggccgaaa tcgagctggg
     1621 cgtgggcgac aagcttctgt tctacaccga cgggctgctg gacatctgcg aatcttcccc
     1681 cacggtggaa gcgctcggcc aggccatggt ctcggtagcc gacaagccga acctcctcga
     1741 gcaggtgctc atggaggtga ctcacggccg cgcccgcccg gattgcgacg acgtgaccat
     1801 ggtgctgctg gatgccgctc ccggcgaaag cgtgttcaac gagtctgtgg agggactcga
     1861 gctgcaggag atgccgacca cggcctcgcc gacgatcagc tgggcagcct cggaggacgg
     1921 ggtctttctc atcctggagg gctgggtgac ctggctgtgc ggggagacgt tgttcaatgc
     1981 ggccatgccg ttgatcaaag cgcaccggcg ccttgtcatc gacctcgagc tttgcgagca
     2041 catggacagc accatgttcg gcaccctgca cgagctgatc caggaagccg accggctcgg
     2101 atccgcggtg gtcctgcagc atgtgggccc gcggcagatg gaagatttcg aggagctcag
     2161 cat
//
LOCUS       DBNHKV010000327        10151 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_969068, whole genome shotgun sequence.
ACCESSION   DBNHKV010000327 DBNHKV010000000
VERSION     DBNHKV010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10151)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10151
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_969068"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="AC16PP_10220"
     CDS             complement(<1..219)
                     /locus_tag="AC16PP_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482043.1"
                     /translation="MAEAIPTREIQSWPEQALRGHRIPGSRYTSKEFAEKEWEGMWTK
                     VWLLLGREEELPNPGDWQCEEVGPESILM"
     gene            327..968
                     /locus_tag="AC16PP_10225"
     CDS             327..968
                     /locus_tag="AC16PP_10225"
                     /inference="COORDINATES: protein motif:HMM:NF018717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR6482044.1"
                     /translation="MSRPAYMIIAIDVRDPSRMAEYAGGTQPLLEAAGARVLAATNGI
                     TVEDGVWARERIALLEFPSMESARSFWSADAYAPLKALRESISTSDVVLVEGTQDERV
                     GEEDGAHYLLGASTMLNADWVEEYIQKVPPISARFGVKMLAGGPEFETLEGAWPGESV
                     VVLRFPSEHAFREFWYGDDYRPMKELREANAPGHHISFAETFELPDAGGPSQG"
     gene            1042..3678
                     /locus_tag="AC16PP_10230"
     CDS             1042..3678
                     /locus_tag="AC16PP_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR6482045.1"
                     /translation="MSPIAPLVVTPTARLARTLSRRVALERAAGGAGAWVPPRIQSFP
                     AWLANQREDYFLTATDHRVPIGAHQALWLWQSLIDRQVFIGDPEVAALAQRAWRLIHE
                     HALEAPERWPELMLSEDARAFRGWAAAYRTECDRRGVVDEWAFAGELPGLIEAGHLAI
                     PQGLELAGFDLPMTPLQVRIFDALEAMGCAVSGRPEGSASRTSQTAAMELIRFHLPDD
                     ELRGAAGWARSVLEAEPGRTVAVVVPDLAGRLERVESIFREVFDPPASVLGPRGAEPF
                     HVSLGRRLSEWSLVADALAFLELGSQRLTQPQAGRLLRSPFLAGWPDETLDRSRALAR
                     LVSRAPFEVTVNELRWALDGVGAAALDEALQSWQTLREAAAQSAWPSDWAGRFQQELS
                     ALGFASGRPLDSREYQVLNRWHDLLESFGALDVVAAGPWSRTQALRQLAERARSSVFR
                     ERNPGVPVEVLGVEEALGSSFDAVWITTLDSATWPGSPVREPLIPAAVQASVPRASGD
                     GCLARAQLELDALCASAPDVRGSYARGVEDQALEITGLLRHLPVIDAPPPTPAPGARF
                     AEPFEDHEAPKRPAGPVSGGTGVLRDQSDCPFRAFARWRLGAVDLTPPRPGLDAAQRG
                     TVIHRALERFWEDLDGQAALQGLDRDAREHRIAAAAAAAVDDLVSRHRAILGPGARRL
                     EERRVARALTRWLGLEVQRAGFEVVDREGEVAMTFAGLELEGKIDRLDRLPDGRLMLI
                     DYKTGRTSRNAWLPEARIVDPQLPAYAVSMPSAPAAITFARIRADDSRFDGIADGDPG
                     TAGVIPLADAGPRYGELGSWQALLSGWRGLLDALAQDFRSGRAAVDPRDADACKTCHL
                     HALCRIRDRDPYRDGEGGSEDD"
     gene            3671..7000
                     /locus_tag="AC16PP_10235"
     CDS             3671..7000
                     /locus_tag="AC16PP_10235"
                     /EC_number="3.1.11.5"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR6482046.1"
                     /translation="MTDRRARAEAVEPDRSVIVQAPAGSGKTTLLVERFLGLLGTVEA
                     PEEVLAITFTRKAAAEMRERVLRFLDPDFQSDEPHEQGPLNRAREVRDRVAAWRLLEN
                     PQRLLIRTIDSFNYYLARTMPVASALGPVPAPADDTASLYREAARGVLSLVGTSDGVA
                     PALELLLDWRDHQSQGVEDLLAGLLAQREQWLRAAGIAGDAERGVLEAALEEVVRGRL
                     ERADAALNAALSAAGFSSAELAGLLRVAARTLRDENRESTTLVFLEADGLPRPDPERI
                     DHWNALVAALLTANLSKPAFRRSVNVSNGFQRQSEEKAEFEALLGAFGENAGLLEALH
                     RARGLPAPRYDDESWQVLDALVQVLKRAAVELETLFARRGRTDYAGLAAAALRGLGDE
                     DGVTDLGLYLDRRIRHVLVDEFQDTNFSQLHLLEKLTAGWEPGDGRSLFLVGDPMQSI
                     YRFREAEVGLFIRVRDQGLGPLSLESKRLASNFRSREEIVRWVNDHLGPIFPEVEDAA
                     AGAVAYAPSDGERGAGGAVEVLARSERSAEAEAVADLVSRALDDHRDDAEYRAAIIVR
                     ARSHLAEILPALVRRGVPYRAVKLDPLGNRPVVQDLLALTRVILQPADVAALLAVLRS
                     PVCGLTLADLHALAGDGRSPLEADALARLAGEPRRRAARVFDVLGDARLAWRRFSVRA
                     LVDGAWRRLGGPECCPDEAARRDAAVYLDALDAAERGGLLEDWNDFRESLAGRHAEGD
                     PQSEGVKLEVLTMHGAKGLEWDLVVLPALDRAPGRPASTMLHWLPFLEADGREEVLLA
                     PLRAAEVAQDPPLVKLIRAEQQVRERYENQRVLYVACTRARERLVLSATIDPDKDPFT
                     PPSGSLLADLWATAGAEFEAALDTSGGAAAGPTVPDQRLNRIAADWEPPLGDRVAWKP
                     ELETRERAAPVEYNWAGLQARRIGTVVHRLLERVGQVGAEDFTDAHREHLLTRIPVLL
                     QSMGTRPDDLDRSAAVVADALARTLDSETGRWLLSGSHEEGACELPIAGMIDGRLVNA
                     VVDRTFVDADGVRWIVDYKSGYHAGSDLEDFFAQERERYQEQLDGYRRLFEQLEDRPV
                     KTALYLPRHGRLEVVAPAGYP"
     gene            complement(7026..7673)
                     /locus_tag="AC16PP_10240"
     CDS             complement(7026..7673)
                     /locus_tag="AC16PP_10240"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR6482047.1"
                     /translation="MGKISLTIDNGPHPTVTPEVLDALADRQVTATFFLIGQEAARPG
                     GLDLIEHISKAGHPIGNHTWSHTVAFGLNPGASAIADEVARTQKLIAPFASQPPMFRP
                     AGGGGRLDSSLLSAELIDYLCRERYTCALWNVVPRDWEDPQGWVNRALREVGTLDWSV
                     VVVHDVIDGNADQIAAFIDRARDDGHEFVTDIAPECLPIVGGTITADLAALTGSR"
     gene            7818..8963
                     /locus_tag="AC16PP_10245"
     CDS             7818..8963
                     /locus_tag="AC16PP_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HIR6482048.1"
                     /translation="MSADRILTTHTGSLPRPDDLIQMMWAKGDGIPVDEGALAERVAA
                     AVQETVDKQVAAGISIVNDGEMSKPSYATYVKDRLSGFGGESPERYYFADLDAYPRSA
                     EIVAANPGRRKRSAPACNAPIGVQDAEAAALDMVNLCRSAEGADVDGIFSSAASPGVV
                     SLFFGNEYYASDEEYVFAIAEAMRHEYEAIAAAGATVQLDCPDLAMGRHTSYADMDLA
                     AFRKRVAMNIEALNHGVRNIPPEQLRMHLCWGNYPGPHHCDVAITEIIDLVWQARPRT
                     VLFEAANPRHAHEWRIFETVGIPDDKVICPGMIEPQSNYIEHPELVAQRIERYANLVG
                     RDRVMAGVDCGFSVHVGMQGIDPDVAWAKLASLAQGAEIASARLWGG"
     gene            9055..>10151
                     /locus_tag="AC16PP_10250"
     CDS             9055..>10151
                     /locus_tag="AC16PP_10250"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6482049.1"
                     /translation="MPAIFFALAALGLLWMPGTVAVAEVSDQSGIIEEMVVTTARKRE
                     EAVEDIPASINVFSEDEIRSAGIETPHDFIALTPNVTLVQTQNAGNSFINIRGVSQAR
                     NSEMSAAVLVDDVLMTNPAQFNQQLYDIEQIEVLRGPQGALYGRNAIGGAITIRTKAP
                     TDEFEGIIDVGGDSGPGYKVRGAVSGPISDTLKFRASASYVDTDGYINNKYLDEEADP
                     YEDLSARLRLLYEPTDTFSADLRFYISQMDTQALYFNLAAEADDTSLPVRVNNPGVNE
                     RDTWGFSLKLDFETEFGTLTSITAFDDLEELLTGDNFDFLPREESWLNGPFTFGFGPY
                     MKFLTGDDVVDLSQTQYLEVEAWSQEIRFTSP"
BASE COUNT         1544 a         3314 c         3537 g         1756 t
ORIGIN      
        1 catcaggatg gactcggggc ccacctcctc gcactgccag tcgccggggt tcggcagctc
       61 ctcttcccgg cccagcagca gccagacctt ggtccacatg ccttcccact ctttctcggc
      121 aaactccttc gaggtgtagc gggatcctgg tatccggtgc ccgcgcagcg cctgctcggg
      181 ccagctttga atttcgcggg ttggaattgc ttcggccatt gtgctttctc tcgctctgac
      241 gttttgttcg ctccgggctg ccgagtataa cgcgttcact ttcccttatg atgtgcaact
      301 taaagagcgc gtcagggagt cagcccatga gccgaccggc atacatgatt atagccattg
      361 atgtcaggga tccgtcacga atggccgagt acgcgggcgg cacacagccg cttctggaag
      421 ccgccggcgc cagggtgctc gccgcgacca acgggatcac tgtcgaagac ggtgtctggg
      481 ctcgggagcg aatcgccctc ctcgagttcc cctcgatgga gagcgccagg tccttctgga
      541 gcgctgatgc gtacgcgccg ctgaaggcgc tgcgagaatc catcagcacg tcggacgtgg
      601 tcctggtcga gggtacgcag gacgagcggg ttggagagga ggacggtgcc cactacctgc
      661 tcggggcgag caccatgctg aacgcagact gggtggaaga gtacattcag aaggtgcctc
      721 ccatctcggc ccgctttggc gtcaagatgc tggccggagg cccggaattc gagaccctcg
      781 aaggtgcctg gcccggcgag tccgtggttg tgttgcggtt cccttccgag cacgccttcc
      841 gggagttctg gtacggtgat gactaccgcc cgatgaagga gcttcgcgaa gccaacgccc
      901 cggggcatca catctctttt gcggaaacct tcgagctgcc cgacgccggc gggccatccc
      961 aaggttgagg cggtctcccc gcccttggcc gggtactgtc cgatcgagcc gttcggctcg
     1021 ccttgatcgg gtgaggcacg ggtgagcccg attgcgcccc tcgttgtgac gcccaccgcc
     1081 aggctcgccc gaacgctttc ccgtcgcgtg gctctggagc gggcggccgg cggggccggt
     1141 gcctgggtgc cgccgcgcat tcagagcttc ccggcctggc tggccaatca gcgcgaggat
     1201 tacttcctca ccgccaccga tcatcgcgtt cccatcggcg cccatcaggc gctgtggctg
     1261 tggcagtcgc tcatcgatcg ccaagtgttc atcggcgacc ccgaggtggc ggcgctggcg
     1321 cagcgggcat ggcggctcat ccacgagcac gccctggaag cgccggagcg ctggcccgag
     1381 ctcatgctgt cggaggacgc acgggcgttc cgcggctggg ctgccgcgta ccgtaccgag
     1441 tgtgaccggc gcggtgtggt ggacgaatgg gcgttcgccg gggagctgcc gggactcatc
     1501 gaggcgggcc acttggcgat cccgcaggga ctggagctgg ccggcttcga tctgccgatg
     1561 acgccgctgc aggtccgcat cttcgatgcg ctcgaggcta tgggctgcgc ggtctccgga
     1621 cgccccgaag ggtctgcgtc ccgaacttcc cagacggcgg caatggagct catccgcttt
     1681 catcttcctg acgacgagct gcgcggcgcg gcgggctggg cgcgatctgt gctggaagcg
     1741 gagccggggc gaacggtcgc cgtggtggtc ccggatctgg cgggacggct cgagcgggtg
     1801 gagtccattt tccgggaggt gttcgacccg ccggcatcag tccttggtcc gcgaggtgcc
     1861 gagccgtttc acgtttccct gggcagacgc ctctcggaat ggtccctggt tgctgacgcc
     1921 ctggcctttc tggagctggg cagccagcgg ctcacccagc ctcaggcggg ccgattgctg
     1981 cgctctccct ttctcgccgg ctggcccgac gagacgctcg atcggtcccg ggcgctggcg
     2041 cgcctggttt cccgggcacc cttcgaggtg accgtcaacg agctgcgctg ggcgctcgac
     2101 ggggtcggtg cggcagccct cgatgaggcg ctccagagct ggcaaacgct gcgcgaggcg
     2161 gctgctcagt cggcatggcc gtcggattgg gcgggccgat ttcagcagga gctgtcggct
     2221 ctcgggttcg cctcggggcg gcccctggac agccgcgagt atcaggtgct caatcgctgg
     2281 cacgatctgc tggagagttt cggcgctctg gacgtggtgg ccgccgggcc ctggtcccgt
     2341 acgcaagccc tgcgacagct tgcagagcgt gcccgatcca gcgtctttcg tgagcgcaac
     2401 cccggcgtgc ccgtggaggt tctcggcgtc gaggaggcgc tcggctccag cttcgatgcg
     2461 gtttggatca ccaccttgga cagcgcgacg tggccgggtt cgccggttcg cgaaccgctg
     2521 ataccggctg ccgttcaggc atccgtgccg cgggcgagcg gagacggatg cctggccagg
     2581 gcccagctgg agctggacgc gctttgcgcg tcggcccccg atgtccgcgg cagctatgcc
     2641 cggggcgtag aggatcaggc gctggagatc accggcctgc tccgccattt gccggtcatt
     2701 gacgcgcccc cgccgacgcc ggcgcccggg gcacgttttg cagagccgtt cgaggaccat
     2761 gaggctccga agcgcccggc cggtcccgtc agcggcggaa ccggcgtgct gcgtgaccag
     2821 tccgactgcc cgttccgggc attcgcgcgc tggcgactcg gtgcggtgga tctcaccccg
     2881 ccgcgcccgg ggctcgacgc ggcccagcga ggcaccgtga ttcatcgtgc cctcgagcgc
     2941 ttctgggaag acctggatgg ccaggccgca ctgcaggggc tggatcgcga cgcccgcgag
     3001 catcgcatcg cggccgcggc cgcggccgcc gtggacgatc tcgtgagccg gcaccgcgcc
     3061 attctcggcc cgggcgctcg ccgtctcgag gagcgccgcg tcgctcgggc acttacccgg
     3121 tggctggggc tggaggttca gcgagccggt ttcgaggtgg tggatcggga aggcgaggtg
     3181 gcgatgacgt tcgcgggcct ggagctggag ggcaagatcg atcgccttga tcggctgccc
     3241 gacgggcggc tgatgctcat cgactacaaa acgggccgca cgtcccgcaa tgcctggttg
     3301 ccggaagcgc gcatcgtgga cccccagctg ccggcctatg cggtgtccat gccctcggcg
     3361 ccggccgcca tcacttttgc gcgtatccgc gcggacgatt cgcgctttga tgggattgcc
     3421 gacggtgatc cgggtaccgc cggggtgatc ccgctggcgg atgccggtcc ccgctacggg
     3481 gagctgggct cctggcaggc gctcctgtcc ggctggcgcg gcctgctcga tgcgctggcc
     3541 caggacttca ggtcgggtcg cgctgccgtg gatccgagag acgccgacgc ctgcaagacc
     3601 tgccacctcc acgcgctgtg ccgcatccgg gaccgggatc cttaccgcga cggggaaggg
     3661 ggctcggaag atgactgatc gaagagcacg cgccgaggcc gtggagccgg atcgctccgt
     3721 catcgtccag gctccggccg gatcgggcaa gaccacgctg ctggtcgagc gttttctagg
     3781 cctgctggga acggtagagg cgccggaaga ggtgctggcc attaccttta cccgcaaggc
     3841 ggcagcggag atgcgtgaac gtgtgcttcg cttcctggac cccgacttcc aatccgatga
     3901 gcctcacgag cagggtccgt tgaaccgcgc ccgcgaggtt cgtgaccggg tggcggcctg
     3961 gcggcttctg gagaatccgc aacgtctgct catccgcacc atcgacagct tcaactacta
     4021 cctggcccga accatgccgg tggccagcgc cctgggtcct gtcccggcgc ccgccgacga
     4081 cacggcgtcg ctttaccgcg aggccgcacg cggcgtgctg agcctggtcg gaacctccga
     4141 cggggtcgct cccgccctgg agcttctgct ggactggcgc gatcaccaga gtcagggcgt
     4201 ggaggacctt cttgccggcc tgctagccca gcgggagcag tggttgcggg cggcgggcat
     4261 tgccggcgat gcggagcgcg gcgtgctgga agccgccctg gaggaggttg tgcggggcag
     4321 gctcgaacgg gccgatgcgg ccctgaacgc ggcgctctcc gcggccggtt tctcctctgc
     4381 tgagcttgcc ggcctgctgc gggtggcggc ccgcacgctg cgcgacgaga accgtgaatc
     4441 caccaccctt gtcttcctgg aagccgacgg gctgccccgg cccgatcctg agcgcatcga
     4501 tcactggaac gcgctggtgg ccgccttgct caccgcgaac ttgagcaagc cggcctttcg
     4561 gcgcagcgtg aacgtgagca acgggtttca gcgtcagagc gaggagaagg cggaattcga
     4621 agcgctcctc ggcgcgttcg gggagaacgc cgggttgctc gaggccctcc accgggcccg
     4681 cggactgccg gcgccgcgct acgacgacga gagctggcag gtgctcgacg cgctcgttca
     4741 ggttctcaaa cgtgccgccg tagagctgga gacgctgttt gcccggcgcg ggcgaactga
     4801 ttacgcgggg ctcgctgctg ccgctctgcg ggggcttggc gacgaggacg gcgtgaccga
     4861 cctcggcctc tatctggacc ggcgcatcag gcacgttctc gttgacgagt ttcaggacac
     4921 caacttcagc cagttgcacc tgctggagaa gctcacggca ggctgggagc cgggcgacgg
     4981 ccgcagcctc ttcctggtgg gcgacccgat gcagtcgatc tatcgctttc gggaggcgga
     5041 ggtgggtctg ttcatccgtg tccgggacca gggccttggg ccactttcgc tggagtcgaa
     5101 gcggttggcg agcaacttcc gctcccggga ggagatcgtc cgctgggtca acgaccacct
     5161 gggtccgatc tttccggagg tggaggatgc cgctgctgga gccgtggcct atgcgccttc
     5221 cgacggggag cggggcgctg gcggtgccgt ggaggttctt gcccgatccg aacggtcggc
     5281 cgaagccgag gccgtggccg atctggtcag tcgcgccctg gacgaccatc gagacgatgc
     5341 ggaataccgg gcggccatca tcgttcgggc ccgcagccat ctggcagaaa tcctgccggc
     5401 gctggttcgc cgcggggtgc cctaccgcgc ggtgaagctg gaccccctcg gcaaccggcc
     5461 cgtcgtgcag gatctgctgg ccctgaccag ggtgatcctc cagcctgcgg acgtcgccgc
     5521 tctgctcgcg gtgctgcgtt cgcccgtatg cgggctgacc cttgccgatc tgcacgcgct
     5581 cgccggtgac ggccgctcgc cgctggaggc cgatgccctt gcacgcctgg cgggagagcc
     5641 ccgacgacgg gctgcgcggg tcttcgacgt gctgggcgac gcgcgcctgg cctggcggcg
     5701 cttctccgtt cgtgcgcttg tcgacggggc ctggcgccgg ctcggtgggc ccgaatgttg
     5761 ccccgacgag gcggcgcggc gtgatgccgc cgtgtatctg gatgcgttgg atgccgccga
     5821 gcggggcggt cttctggagg actggaacga cttcagagaa agcctcgccg ggcggcacgc
     5881 cgagggcgac ccgcaaagcg agggggtcaa gctggaggtt ctcaccatgc acggggccaa
     5941 aggcctggag tgggatctgg tggtcctgcc ggcactggat cgggcccccg gccgtccagc
     6001 atcgaccatg cttcactggc tgccgttcct cgaggccgat ggtcgggagg aagttctgct
     6061 ggcccctctg cgggcagcgg aggttgctca agacccgcct ttggtgaagc tgatccgtgc
     6121 ggagcagcag gtgcgggagc gctacgagaa ccagcgtgtg ctctacgttg cgtgcacgcg
     6181 ggcccgggag cggctggtgc tctccgccac catcgatccg gacaaggacc ctttcacgcc
     6241 gccctcaggc agtctgctcg ccgacctctg ggccacagcc ggcgcggagt tcgaggccgc
     6301 cctcgacacc agcggtggcg ctgccgccgg gcccacggtt ccggatcaga ggctgaatcg
     6361 gatcgcggca gactgggagc cgccgcttgg cgatcgggtg gcctggaagc cggagctgga
     6421 gacccgggaa cgagccgctc cggtggaata caactgggcc ggtctgcagg cgcgccggat
     6481 cgggacggtg gtgcaccggt tgctggagcg ggtgggtcag gtgggtgccg aggacttcac
     6541 tgatgcccac cgcgagcatc tgctgacccg catacccgtc ctcctgcagt ccatgggaac
     6601 gcgacccgac gatctggatc ggtctgcggc ggtggttgcc gacgcgctgg cacgaaccct
     6661 tgacagcgag accgggcgct ggctcttgag cggcagccac gaggaaggcg cgtgtgagct
     6721 tcccatcgcg ggcatgatcg acgggcgact ggtcaacgcc gtcgtcgacc gcaccttcgt
     6781 ggacgcggat ggcgtgcgct ggatcgtgga ctacaaatcc ggctaccacg ccgggtcgga
     6841 tctggaggac tttttcgccc aggagcgcga gcgctatcag gaacagctcg acggctaccg
     6901 gcggcttttc gagcagctcg aggaccgccc cgtgaaaacg gcgctgtacc tgccgcgtca
     6961 cgggcggttg gaggtggtgg ctccagccgg ttacccgtaa acgccgcggc ccgcaggaga
     7021 tggttctaac gtgagccggt caaggccgcc aggtcggcgg tgatggtccc tcccacgatg
     7081 ggcaggcact cgggcgcgat gtccgtgacg aactcgtggc cgtcatcccg ggcccggtcg
     7141 atgaaggcgg caatctgatc ggcgttcccg tcgatgacgt cgtgaacgac caccacgctc
     7201 cagtccagcg tgccgacctc tcgaagcgcc cggtttaccc agccctgcgg gtcttcccag
     7261 tctcggggca ccacgttcca caacgcgcag gtgtagcgct cgcggcagag gtagtcgatg
     7321 agctccgctg agaggaggct cgagtctagc cgcccgccgc caccggcggg acggaacatg
     7381 ggtggctggc tcgcaaaggg cgcgatcagc ttctgcgttc gggcgacctc gtcggcgatg
     7441 gccgacgcgc ccgggttcag gccgaacgca acggtgtggc tccaggtgtg attgccgatc
     7501 ggatggcccg ccttgctgat gtgctcgatg agatccagcc cgccgggtcg tgccgcctcc
     7561 tggccaatca gaaagaacgt ggcggtgacc tgcctgtcgg cgagcgcgtc gagcacttcg
     7621 ggagtgactg tcggatgcgg tccgttgtcg attgtcaggg agatcttgcc caaggatttg
     7681 gtcctggccg gttgggtcgc tcgagagtcg ggggatctta gcagaaggcg cttgccgagt
     7741 ttcggcggcc accggctgcg gctgggagcc gctttgcaga tgagctaatc ttcgggcgga
     7801 aggccacggg gaagcacatg agcgcagacc gaattctcac tacccacacg ggcagcctgc
     7861 cgcgcccgga tgatctcatt cagatgatgt gggccaaagg ggacggcatc ccggtagacg
     7921 agggggcgct tgccgagcgg gtcgccgcgg ccgtccagga gaccgtggac aaacaggtgg
     7981 cggcgggtat cagcatcgtc aacgacggcg agatgtcgaa acccagctac gccacctacg
     8041 tgaaggatcg gctcagcggg tttggcggtg agtctccgga gcgctactac ttcgccgacc
     8101 tcgatgcgta tccccggagc gcggagatcg ttgccgccaa tcccggccgg cgcaagcgct
     8161 cggccccggc ctgcaacgcg ccgatcggcg tgcaggacgc cgaagcagcc gctctggaca
     8221 tggtcaacct gtgccggtcg gctgaaggcg ccgacgtgga cgggatcttc tccagcgccg
     8281 cctcgccggg tgtggtctcc ctgttcttcg gtaacgagta ctacgcctcc gatgaggagt
     8341 acgtgttcgc catcgccgag gccatgcgcc acgagtacga ggccattgcc gccgccggcg
     8401 ccacggtcca gctcgactgc cccgatctcg ccatgggaag acacacctcc tatgcggaca
     8461 tggatctcgc cgctttccgc aagcgggtgg ccatgaacat cgaagcgctc aaccacggcg
     8521 tgcgcaacat ccctcccgag cagctgcgga tgcacctgtg ctggggcaac tacccggggc
     8581 cgcatcattg cgacgtggcc atcaccgaga tcatcgatct ggtctggcag gcgagaccgc
     8641 gcaccgtgct tttcgaggcc gccaatccga ggcacgcgca cgagtggcgc atcttcgaga
     8701 ccgtcgggat accggacgac aaggtcatct gccccggcat gatcgagccc cagtccaatt
     8761 acatcgagca tccggagctg gttgcccagc gcatcgagcg gtatgcgaat ctcgtcggcc
     8821 gggatcgggt gatggcgggc gtggattgcg gtttcagcgt tcacgtgggg atgcagggca
     8881 tcgatccgga cgtggcctgg gcaaagctcg cctccctggc ccagggtgcg gagattgcgt
     8941 ccgctcggct ctggggaggt tgaacggccg cgctgggtgt ttgaattggc cgcaacgagg
     9001 ccgtagcatc cgatgactcg cccgttgagg cgaaggaatc aagaggggat aaagatgcct
     9061 gcaatatttt tcgcgctggc cgcgctcggg ctgttgtgga tgcctgggac cgttgccgtg
     9121 gccgaggtgt ccgaccagtc cggaatcatc gaggagatgg tggtgaccac ggcccggaaa
     9181 cgggaagagg ccgtcgagga catcccggca tcgatcaacg tcttctccga ggacgagatc
     9241 cgttccgccg gcatcgaaac cccacacgac ttcatcgcgc tcacacccaa cgtcacgctg
     9301 gtgcagacac agaacgcggg caactcgttc atcaatatcc gcggcgtctc ccaggcacgt
     9361 aacagcgaga tgtcggcggc cgtgctggtg gacgacgtgc tcatgaccaa cccggcccag
     9421 ttcaaccagc agctctacga catcgagcag atcgaggtgc tgcgcggccc ccagggggcg
     9481 ctctacggcc gcaacgccat cggcggcgcc atcaccatcc gcaccaaagc gccgacggac
     9541 gagttcgagg gcatcatcga cgtgggcggc gactcgggcc cgggctacaa ggtgcgcggc
     9601 gcggtgagcg gccccatctc ggataccctg aagttccgcg cctcggcttc gtatgtggac
     9661 acggacggct acatcaacaa caaatacctc gacgaggagg cggaccccta cgaggatctc
     9721 tcggcccgtc ttcgccttct ctatgagccc acggacacct tcagcgccga cctgcgcttc
     9781 tacatctccc agatggacac ccaggcgctt tacttcaacc tcgccgcgga ggccgacgac
     9841 accagcctgc cggtgcgggt gaacaatccc ggggtcaacg agcgggacac ctggggcttc
     9901 agcctgaagc tcgatttcga gacggagttc ggcaccctga cctccatcac cgcgttcgac
     9961 gatctcgagg agctgctcac cggcgacaac ttcgactttc tcccgcggga ggagtcctgg
    10021 ctgaacgggc cgttcacctt tggctttggc ccctacatga aatttctgac cggggatgac
    10081 gtggtcgacc tcagccagac tcagtacctt gaagtggagg cctggagcca ggaaatccgc
    10141 ttcacctcac c
//
LOCUS       DBNHKV010000328         6407 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_188045, whole genome shotgun sequence.
ACCESSION   DBNHKV010000328 DBNHKV010000000
VERSION     DBNHKV010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6407)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_188045"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..954
                     /locus_tag="AC16PP_10255"
     CDS             136..954
                     /locus_tag="AC16PP_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6482050.1"
                     /translation="MPITIANGIDLYFERRGDGAPLLFISGSHGDLRARPNQFDTPLA
                     RAFDMIAYDQRGLGQTGKPPGEYTMAQYADDAAALLDALRLDAVPVVGVSFGGMVAQE
                     FALRHPDRVDRLILACTSSGGAGGSSYPLHELQALPPRERAEAHLRISDLRHDDEWVA
                     ANPERWERLVELTMAARRPDRDEQGAAKQLRARKGHDTYDRLPRLDVPVLLAGGRRDG
                     IAPVANMEALHRQIAGSRFELFDGGHMFLIQDKTAYPFIVGWLQNDWSELRDEP"
     gene            951..2225
                     /locus_tag="AC16PP_10260"
     CDS             951..2225
                     /locus_tag="AC16PP_10260"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6482051.1"
                     /translation="MSDARSVADYHFLKLNDEVLQNPYPFFRRLREEGGVVREADYDT
                     FLVGSLDAIREVNRQPEVFSSVLVANAPYVPLPCPLDEIAQWRDANPLSDKILSNDPP
                     DHTRHRKLVNRFFTTSRVRDLEPRIRQLTNEIIDTFVDLGEVEFVFGFAHMLPRLVVG
                     ELIGLPEQDQPRFRRHFEDRLALMARAAEKPLEGFQQRTTQASLTEDTFLRDYFVDAI
                     NERRERPRDDIMSELARATFEDGAPVPLESLVSMISLLYGAGGDANTPELMTNAMLVL
                     LDRPDLERALRDDPSLSKAFIEEVLRYDTPVPGVFRIALQNTSIAGTPVPKGSKVMVV
                     YGAANHDEAYFDDPETFVMGRDCPHPHLGFGLGVHFCPGAPLARLEGQVAVQELLRRL
                     TNIRRADNEPIPYVPTVIQRIPIQVRLRFDKA"
     gene            2405..2812
                     /locus_tag="AC16PP_10265"
     CDS             2405..2812
                     /locus_tag="AC16PP_10265"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR6482052.1"
                     /translation="MKIKAVESYADPSARASFTNRRGSARQQSSERLFAQVVLSEQVD
                     GNSLGGATLRCTTVDCSPQGIAFVAERPIPIGAMVDIWLDTRTRPGKMFLSGQVCWCS
                     AGPTPNADSGHQVGVQLLSSPATDLQEWQSILS"
     gene            3454..4497
                     /locus_tag="AC16PP_10270"
     CDS             3454..4497
                     /locus_tag="AC16PP_10270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6482053.1"
                     /translation="MKILSTRRLLQQGNGSFAACCALVVLSTLQASCATHSPVGIEQR
                     AEVDLYESLVDVRFVTNRKLVASASDGVFFAGEAGELSGGQCRVGIGDRAPRGDVVRI
                     DTRTLESTFDSDGSGRFVIYVHGYGESLSKSCRRAALLQHRLGLEKRLLLFSWPSSNY
                     LTYAQDAVALEQSLDPIRDALIRASSAIGAENIVLMAHSMGARGVVDALRAWGPDHGR
                     FGQIIFVAPDIGREAFLEEVGALREKASEITIYSSDNDMALWLSTTVNGSARLGLTKE
                     MDLQHANLIDVTKAGLKSMSGHTYHIFNPAVAEDLRIVLGTGPADGERAFRRVPGKVD
                     RFWVLEPVVTKSL"
     gene            4809..5432
                     /locus_tag="AC16PP_10275"
     CDS             4809..5432
                     /locus_tag="AC16PP_10275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6482054.1"
                     /translation="MTDLDWSRLYLQRREVARKFGGIWSLPLAKRYHIVLAELPAPRS
                     VLDLGAGDRSLQRRVSALWPEAEYKSCDIDPSADHDFAGIDEVRGTYDLICVLEMIEH
                     VPLDEAGRILEQCHGLLGDGGMIALTTPNVYYPPAFLRDATHRTPFCYDELGGLLQLC
                     GFEVTHVYRLHQDALFKKLAKRYLFYPLYRLLGLDFAHGVMVVARKR"
     gene            5435..6391
                     /locus_tag="AC16PP_10280"
     CDS             5435..6391
                     /locus_tag="AC16PP_10280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6482055.1"
                     /translation="MRVLFVSKNRAGPTARYRVLPLIRKLRSEGVETEHMDEFTVIAK
                     LGLLRRANEFDLVFVQRRLLAGWLLAAIRKPLVFDFDDAIFCRSNGEESRRRLKRFRR
                     MVARADLVLAGSRYLAERCEGKDAVVVPTAIDAERYVAGPKTGGDLVLAWVGSRSTGR
                     YLEGHRRELEQIGDAFPGIRLKVVADFEFNLSNITVDNIPWSTEAEVSTLAEADIGIA
                     PLPDNPWTQGKCAFKVLQYMAASLPVIASPVGANRDVVVDGKTGILANSAQQWCRAVE
                     RLGDRDLREQMGAAGRRRVETDYAESVMVKRVLGTLRGRGLL"
BASE COUNT         1167 a         1858 c         2076 g         1306 t
ORIGIN      
        1 gaggtcgacg gttcgatccc gtctggctcc accaattaaa tcaatgactt gaggttggag
       61 accccccctt tcctgaccgg tggccttgat gcggcgcttg gcatataaac gaacgtgtca
      121 gcgccgaggg ccagcatgcc aatcaccata gccaacggga tcgacctcta cttcgagcgt
      181 cggggcgatg gcgcgccgct gctgttcatc agcgggagcc acggcgacct gcgcgcccgg
      241 ccgaatcagt tcgatacccc gttggcccgg gccttcgaca tgatcgccta cgaccagaga
      301 gggctcggcc agaccggcaa gccgccggga gagtacacca tggcgcaata tgccgacgac
      361 gcggccgcat tgctggacgc gctgcggctc gatgcggttc ccgtggtcgg cgtttcgttc
      421 ggcggcatgg tcgcgcagga gttcgcgctt cgtcatccgg accgtgtcga ccgcctgatt
      481 ctcgcgtgca cgtcgtcggg aggcgctggg ggatcctctt acccgctcca tgagcttcag
      541 gcgctgcctc cccgggagag agccgaagcc catctccgga tctccgatct gcgtcacgac
      601 gacgagtggg ttgcagcgaa cccggaacgc tgggagaggc tggtggagct gacgatggct
      661 gcgcgacgcc cggacagaga cgagcagggc gcggccaagc agctccgggc ccgcaagggg
      721 catgacacgt acgatcgtct gccccgactc gacgttcccg ttcttctggc cggtggcagg
      781 agggacggca tcgcgcccgt tgccaacatg gaagccctgc accggcagat tgccggaagc
      841 cggttcgagc ttttcgatgg cggccacatg ttcctgattc aggacaagac cgcctatccg
      901 tttattgtgg gatggctcca aaacgactgg agtgagttaa gggacgagcc gtgagcgatg
      961 caagatctgt agccgactac cactttctca agctcaatga tgaggttctg cagaatccct
     1021 atcctttctt tcgccggctg cgcgaggagg ggggtgtagt ccgcgaggcg gattacgaca
     1081 cgtttctcgt tggttctctt gacgccatcc gtgaagtcaa ccgccagccg gaagtgttct
     1141 cgagcgtcct ggtagcgaac gcgccctacg tgccgctgcc ttgcccgctc gatgagatcg
     1201 cccagtggcg ggatgccaac ccgctcagcg acaagattct ctccaacgat ccgcctgacc
     1261 acacccgtca ccgaaagctg gtgaaccgat tcttcacgac cagccgcgtc cgcgacctcg
     1321 agccgcgcat ccgccaactg acgaacgaga tcatcgacac cttcgtcgat ctcggcgaag
     1381 tggagttcgt gttcgggttc gcgcacatgc tgccacggct ggtggtcggc gagctgattg
     1441 gcttgcccga gcaggatcag ccgcggtttc gccgtcactt cgaggaccgg ctcgcgctga
     1501 tggccagggc cgcggaaaaa cccctggagg gctttcagca gcgcaccacc caggcaagcc
     1561 tgaccgaaga cacttttctg cgcgactatt ttgtcgatgc catcaatgag cgacgggagc
     1621 ggccgcgtga cgacatcatg agcgagcttg cccgggccac gttcgaggat ggagcgccgg
     1681 ttcctctgga atcgctggtc agcatgatct cgctgcttta cggcgcgggg ggtgatgcca
     1741 acacgcccga gctcatgacc aacgccatgc tggtgctgct ggatcggccc gacctagagc
     1801 gtgcgctgcg tgacgatccc tcgctgagca aggctttcat cgaggaggtg ttgcgttacg
     1861 acaccccggt ccccggggtg ttccgaattg cgctgcagaa cacgtccatt gcgggcactc
     1921 ccgtgccaaa gggctccaag gtgatggtgg tctacggcgc cgccaatcat gacgaagcct
     1981 atttcgatga tcccgagacg ttcgtgatgg gccgcgactg tccgcacccg cacctaggct
     2041 ttggtctcgg agtacacttc tgtccgggtg cgccgctcgc tcggctggaa ggtcaggtgg
     2101 cagttcagga attgctgcgt cggctgacca acatacgacg agcagacaat gagccgatcc
     2161 cctatgtgcc caccgtgatt cagcggatac ccattcaggt gagacttcgt ttcgacaagg
     2221 cttgaccgcg ccattacgga taattaggta gttttcctga tggtttcaaa acgcgttctg
     2281 gggtgtatga ttcaaaacaa gtcagcggaa cgtttctgct ggcgggggat tgcgctcatt
     2341 caggaagggt gagttgtcag gctaggatgg ccgcgaaata acattggcag aataaaaaga
     2401 cgccatgaaa ataaaagccg tggagtccta tgccgatccg tcggctagag cgagcttcac
     2461 caaccggaga gggtccgcca ggcagcagag ctctgaacgg ttgttcgcgc aggtcgtgct
     2521 ctcggagcaa gttgacggaa acagcctcgg aggtgcgact cttcgatgca ccacagttga
     2581 ctgttcgccc caggggattg cttttgttgc ggagcggcca attccgatcg gcgcgatggt
     2641 cgacatctgg ttggatacac gcacgcgccc tggaaagatg ttcctctcgg gacaggtttg
     2701 ctggtgttcg gctgggccga ccccgaacgc cgacagcggc caccaggtgg gtgtccagtt
     2761 actctcgagc ccggcgaccg acctgcagga gtggcagtcg atactgtcct gacgccgccg
     2821 ctctgtgcca tggacgcgcc actgcaaaag atcgccaact tggttagctg gtgagtcgag
     2881 ccggaaactc atgaatgcgt agtgtggttc tggtgctggc cctcgtgtcg ctcgtggtcc
     2941 tggttctatc ggtcgctcac acgcgcgaag tgggagcggc ttccatcgtg tttcgggacg
     3001 gtcttgcctt tgcgggagac gagtcaaacc ccttttccgg tcgcgtcatc gaacgttacg
     3061 acgatggtcg ggtcaaaacg cggcaaagct tcacaagggg acgcgcacaa ggttcgctgc
     3121 gatcttggca ccgaaacgga caaccctgga ttgtcggcag ttatcgtcgc ggactcgagg
     3181 acggcctggt ccgtagctgg agtccgagcg gcgagttgtg gtttgagaaa gagtaccggg
     3241 acggggtgct cgtgggatgc cgcggctatt gggaggggga actggaaccg gattccttct
     3301 ggtgccgctg gtagcgcgac tctcgttcgc tgattgctgg ccctttcggt tgtcggcaga
     3361 acaaggttga gcatgctcat tgtggggcgg gccgtccccg cgtaatctcg acacgaagcg
     3421 gatgcggtcc cgcaaccggt caaagagcga ttgatgaaga tcctgtcgac caggcgattg
     3481 ttgcagcaag gtaacggaag ctttgcggcg tgctgcgcgc tggtggtgct gtcgacgctg
     3541 caggcctcgt gcgcgactca ctctccggtt ggcattgagc agcgggcgga ggtcgacctc
     3601 tatgaaagct tggtggacgt gcgcttcgtc accaatcgca agctcgtcgc ctcagcctcc
     3661 gatggcgttt tctttgccgg tgaggcgggg gagctctccg gcgggcagtg tcgcgtcgga
     3721 atcggtgatc gtgctcccag aggagacgtg gtgcggatcg atacgcggac gctggaatcg
     3781 accttcgatt cagatggttc ggggcgcttt gtcatttacg tgcacgggta cggagagtct
     3841 ttgtcaaaga gttgtcgacg ggccgccctt cttcagcatc ggctcgggct agagaaacgc
     3901 ctgctgctgt tctcgtggcc gtccagcaac tacctgactt atgcccagga tgccgtagct
     3961 ctggagcaga gcctcgatcc catcagggat gcgctgattc gggcaagcag tgccattggc
     4021 gccgaaaata tcgttctcat ggctcacagc atgggcgctc ggggtgtggt cgatgcgctt
     4081 cgggcgtggg ggccagacca tggtcgtttc ggccagatca tattcgttgc gcccgatatc
     4141 ggtcgggaag cctttctcga agaggttggt gccttgcgcg agaaggcttc ggagatcacc
     4201 atctattcat ccgataacga catggccctt tggctctcga ccacggtcaa cggctctgcc
     4261 cggcttggct taaccaagga gatggacctc cagcatgcca acctgatcga tgtcaccaag
     4321 gccgggctga agagcatgtc gggacacacg tatcacatct tcaatccggc ggtggcggag
     4381 gatctcagga tagtgctggg taccggccca gccgatggcg aacgagcatt ccgccgggta
     4441 ccgggcaagg tggatcgatt ctgggtgctg gagccggtag ttacgaagtc tctctgaacg
     4501 gatcgatgag ctcgcctcga cgtagtatcg agacaggcgt gaggatacca tcggccccgg
     4561 acagggtccc tggggagacg aatgaagaga cgctcattcc ttgcgggcgc cgctggcacg
     4621 cttggtcttg gccgggctag cgcccattct cccgttcgta atccccttcc cgatctggtt
     4681 acgctcgagt cctctttctc aggctttccg ggcaacacgc ccatcagccg catctgctcc
     4741 agtccggaga tgtattgcgt cgcgcaacca ccgctggcag gttctctaga gcgcccgggg
     4801 gcatctctat gaccgatctc gattggagca ggctgtacct gcagcgccgc gaagttgccc
     4861 gcaagtttgg cgggatctgg tcgttgcccc tggcaaaacg ctatcacatc gtgctggcgg
     4921 agttgccggc tccccgttcc gtgctcgatc ttggagcggg cgatcggtcc ctgcagagga
     4981 gggtttccgc cctgtggcca gaggccgagt acaagtcctg cgacatcgac ccctcggcag
     5041 atcacgactt tgcagggatc gacgaagtgc gggggactta tgatctcatc tgcgtgctag
     5101 agatgatcga gcacgtgccc cttgacgaag cgggacgtat cctcgagcag tgccacgggc
     5161 tgcttggaga tggtgggatg atcgccttga ccacgccgaa cgtgtactac ccacccgcgt
     5221 tccttcggga tgctacccac cgcacgccgt tctgctatga cgagttgggc gggctgctgc
     5281 agttgtgtgg tttcgaggtg actcacgtgt accgcctgca ccaggacgcc ttgttcaaaa
     5341 agctcgccaa acgttatctg ttctatccgc tttaccggct tctcggcctg gacttcgccc
     5401 acggcgtcat ggtcgtggcg cggaagcgct gaggatgcgg gtgttgttcg tgtccaagaa
     5461 tcgggcgggg cccacggccc gctatcgggt gctaccgctc atccgcaagc ttcgatccga
     5521 aggcgtggaa acagagcaca tggacgaatt cacggtcatt gccaagctcg ggctgctgag
     5581 gcgggcgaac gagttcgatc tggtcttcgt gcagaggcgc cttctggcag gttggctgct
     5641 tgcggccatt cgcaaaccgc tggtgttcga ttttgacgac gcgatcttct gccggtccaa
     5701 tggggaggaa tcccgccgcc ggctgaagag atttcggagg atggtcgcca gggcagatct
     5761 cgtgctggca ggtagccgat atcttgccga gcggtgcgaa ggtaaagatg cggtcgtggt
     5821 gcccacggcc atcgacgcgg aaaggtacgt agccgggccg aagactggcg gcgacctggt
     5881 gctggcctgg gtaggcagcc ggtcgacggg caggtatctg gaaggtcatc gcagggagct
     5941 ggagcagatt ggcgatgctt ttccgggcat tcgtctcaag gtggtcgcgg acttcgagtt
     6001 caacctgtcc aacatcaccg tcgacaacat tccctggtcc accgaggcgg aggtcagtac
     6061 gctggccgaa gccgacatcg gtatcgcccc gcttccggac aacccatgga cccagggcaa
     6121 gtgcgcgttc aaggtattgc agtacatggc cgcctcgctg ccggtgatcg catcacccgt
     6181 cggagcgaac agggatgtgg tcgtcgacgg caagacgggc atccttgcca attctgcgca
     6241 gcagtggtgc agggcggtcg aaaggctcgg tgaccgcgac ctgcgtgagc agatgggcgc
     6301 tgccggtcgg cgccgggtgg agaccgacta tgccgagagc gtcatggtca aacgggtgct
     6361 aggcacgctg cgtggcaggg gactgctctg aaggcatgtg cagaaat
//
LOCUS       DBNHKV010000329         5426 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1322152, whole genome shotgun sequence.
ACCESSION   DBNHKV010000329 DBNHKV010000000
VERSION     DBNHKV010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1322152"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="AC16PP_10285"
     CDS             complement(<1..282)
                     /locus_tag="AC16PP_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482056.1"
                     /translation="MRNVMLIGGIYHPFEEAAPVLARLLESLGIESVITSDVSEAVAE
                     LESADLFTIYALRWRMLNDEKYVPFRKQWAFELPEEDAQAIENFVEAGGG"
     gene            complement(282..1022)
                     /locus_tag="AC16PP_10290"
     CDS             complement(282..1022)
                     /locus_tag="AC16PP_10290"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6482057.1"
                     /translation="MSVRRALVTGAAHGIGAAIATRLGSAGYRVGVLDMDGTAAAEVA
                     AALPDALPLTADVTDADSIDRALDAFGEPPNLLVNNAGIVHFGPVVEESVADFVKVIS
                     VNLIGCYVASRQCAQRMSGGDHIISITSINDRTPAPGSGAYPAAKAGVRQLMRQLAVE
                     LAERGIRANSVCPGFIDAGMSAPFYADPKVRETRARAVPLGRLGTAEDVANAVAFLDS
                     EAGAYVNGHELVVDGGVTHTLLKHLPRD"
     gene            complement(1019..2221)
                     /locus_tag="AC16PP_10295"
     CDS             complement(1019..2221)
                     /locus_tag="AC16PP_10295"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6482058.1"
                     /translation="MDLRITDYRHAHQALCSPHLKQSLYDAGGVIMEDTLLTLHGQRH
                     TRRRHLALRVFRRDYARHYEREVFPKALRDALEPLRGQPSIDVVAFGHAVSMNLTADF
                     AGIDRSEDPEATAALQRLTKKFGEGATLVHSTRAHDQVRAEVREALAEYRGRFLEPSM
                     ARRERLISAWRGSEIPEESLPRDMLTVLLTDRGGLDDDGLAREIAFYLQAGAHSTSNA
                     FAHALWEVLTWLGSDLARRAALAGDALATQRAVHEALRLHPASPVAWRRALEPIDFSW
                     GLHVEPEDHLVVDIAAANRDPEVFGPEADAFQPGRAVLDRRANPFGLTFGIGVHNCLG
                     RDIDAGLPQDSDTEAGSHQYGTVTLLVHHFLSELDAMFDPADLPVPDEDTSRPNWGRF
                     PVRVRWQT"
     gene            complement(2223..3419)
                     /locus_tag="AC16PP_10300"
     CDS             complement(2223..3419)
                     /locus_tag="AC16PP_10300"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6482059.1"
                     /translation="MSLVTVDSYQAANAALKQKDLRQALYDEGAVIMREVLVNLHGDE
                     HRTRRNVETQVFRRDFFRYYEHEVFPRTLEETIAPFLAAGKMDIVDFGYRVMVNLTAD
                     FTGIDRPKKSPEETEQLIKMLRLFGRTATLAHSTEDREPIRRAALDALADFQRDYVDP
                     SVARRQALIASVNRGDLSEEDLPRDILTVLLKNQDTVQLADDVRVREMAFFALAGAHT
                     SIHTLGHVMHEIFTWCASHPEDWSRFETDPLFVQRAVHESIRLHPSSPIAARRALCPV
                     HVAGKDVQADTRVTVDLQKANRDPAAFGADAERFNPDRPRGAKVPPYGLSFGLGMHAC
                     LGLNLAAGALPKPDTDPDDHHYGTVAMIVRTLLAHRARPEPGSPPRKDTTTARDIWAT
                     YPVTLG"
     gene            complement(3416..4720)
                     /locus_tag="AC16PP_10305"
     CDS             complement(3416..4720)
                     /locus_tag="AC16PP_10305"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase family protein"
                     /protein_id="HIR6482060.1"
                     /translation="MKTLLKNANIIDGLGNAPFAGDVLIDEELILAVGAADGEGATVI
                     DVAGKTVMPGLIDAHCHVTLDETHSNDEMFFHRREGLGALVAAYNVQKLLRAGVTGFL
                     DADCIFDIGVDLRDAIEAGIVPGPRMSTGGNALLTSVGGTAGNLIPAEGRVGYAKVVR
                     SRDEIVTEVRRQIKLGVDWIKVHVTGLVPRQKHRGEIQAWSFDELKTVCDTAHELGIP
                     VVGHCRNAKSIADSVRAGMDMILHATFMDDAALEAVIERKVPLVPTFTFQANLADFGD
                     LVGADPNLREIFRREIAESADMIRRAYEAGVPVLCGTETGFSLTPYGEWHYREMEVFV
                     RDMGFTPLEAIRAATSECGRAIGLQDRVGAVEAGRLADLLVVDGDPSKDVTVLGDKTK
                     IERVFLNGAQLDLPPLPARKPIPGWRVSAYSSSQLFWADAHK"
     gene            complement(4717..>5426)
                     /locus_tag="AC16PP_10310"
     CDS             complement(4717..>5426)
                     /locus_tag="AC16PP_10310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6482061.1"
                     /translation="SGPVGLSAIAISCDKVVAKAKLIAAQVLDVPTQSVSFADGEFSS
                     PQSNQRLSFAEVALNAYTAHHFDTTQIEPGLKEGCYFDPPNFNFPAGCHVCEVEIDRE
                     TGVVEIVDFVAVDDFGTLVNPMIVEGQVHGGVAQGIGQALLENTVYDDGGQLLSGSFM
                     DYCMPRADDLPDIRVGFTNTPSPSNPLGIKGCGEAGAIGAPPAVINAITDALGVRELD
                     MPATSEKIWRVIKEQAL"
BASE COUNT          859 a         1768 c         1868 g          931 t
ORIGIN      
        1 cccgccgccg gcttccacga agttctctat ggcctgggca tcttcctcgg gcagctcgaa
       61 tgcccactgc ttgcggaagg gcacgtattt ctcgtcgttg agcatgcgcc agcgcagggc
      121 gtagatggtg aagaggtcgg cggattcgag ttccgccacc gcctccgaga cgtcgctggt
      181 aatgacgctc tcgatgccga gagattcgag gagtcgggcg agcacgggtg ccgcctcctc
      241 gaacggatga tagatgccgc caatgagcat cacgttgcgc atcagtccct cggcaggtgc
      301 ttgaggaggg tgtgggtgac gccgccgtcc acgaccagct cgtggccgtt gacgtacgcg
      361 ccggcctccg agtcgagaaa ggcgaccgcg ttggccacgt cctctgcggt gcccagtcgg
      421 cccaacggaa ccgcgcgggc gcgggtctcc cttaccttcg ggtccgcata gaagggtgcc
      481 gacatgccgg catcgatgaa gccgggacag accgaattgg cgcgaatgcc ccgctcggcg
      541 agctccacgg ccagctggcg catcagctgg cggacccctg ccttggccgc gggataagct
      601 ccagagccgg gggccggcgt ccggtcgttg atggaggtga tgctgatgat gtgatccccg
      661 ccgctcatcc gttgggcgca ctgccggctg gccacgtagc agccgatcag gttgacggat
      721 atcaccttga cgaagtcggc cacgctttcc tcgaccaccg gcccgaagtg cacgatgccg
      781 gcgttgttca ccagaaggtt gggcggctcg ccgaaggcgt ccagcgcccg gtcgatgctg
      841 tcggcgtcgg tcacgtcggc ggtcagcggc agcgcatcgg gcagcgcggc cgccacctcc
      901 gcggccgccg tgccgtccat gtcgagcacg ccgacccggt atccggcgct tcccagccgg
      961 gtggcaatgg cggcgccgat gccgtgggca gcgccggtca ccagcgctcg gcgcacgctc
     1021 atgtttgcca ccgcacgcgg acgggaaagc ggccccagtt gggacgcgag gtgtcttcgt
     1081 cgggcaccgg caggtcggca ggatcgaaca tggcgtctag ctcggataga aagtgatgaa
     1141 ccaggagcgt caccgtgccg tactgatggc tgccggcttc ggtatcggaa tcttgcggca
     1201 ggccggcgtc tatgtctcgg ccgaggcagt tgtgcacgcc gatcccgaag gtcagaccga
     1261 aggggttggc gcgccggtcc agcaccgcgc gcccgggctg gaacgcgtcc gcctcgggcc
     1321 cgaaaacctc cgggtcccga ttggccgcgg cgatgtccac caccaggtga tcctccggct
     1381 ccacgtgcag gccccagctg aaatcgatgg gctcgagcgc ccggcgccag gccacggggc
     1441 tggcgggatg aagccgtagc gcctcgtgca cggcccgctg ggtggcgagc gcatcgccgg
     1501 ccagggccgc cctgcgggcg aggtcgctgc cgagccaggt cagcacctcc cagagcgcgt
     1561 gggcgaaggc gttggacgtg ctgtgggcgc cggcctgaag gtagaaggcg atctcccgcg
     1621 cgagaccgtc gtcgtcgagg ccgccccggt ccgtcagcag caccgtgagc atgtcgcgag
     1681 gcagggattc ctcgggtatc tcgctcccac gccacgccga gattagccgc tctcgccgcg
     1741 ccatggacgg ctccaggaag cggccgcggt actcggccag cgcttcgcgt acctctgccc
     1801 tgacctgatc gtgggcgcgc gtggaatgca ccagggtcgc tccctcgccg aatttcttcg
     1861 tcaaccgctg caacgcggcc gtggcttccg ggtcctcgga ccggtcgatg ccggcgaaat
     1921 cggctgtcag gttcatggat acggcgtggc cgaacgccac gacgtcgatg ctgggctggc
     1981 cccgcagcgg ctccagggcg tcgcgcagcg cttttggaaa aacctcgcgc tcgtagtgac
     2041 gggcgtagtc tctgcggaag accctcaggg cgaggtgccg tcggcgggtg tggcgctggc
     2101 cgtgcagggt cagcagcgtg tcttccatga tcaccccgcc ggcgtcgtaa agggactgtt
     2161 tcagatgcgg gctgcacaac gcctggtgag cgtggcggta gtcggtgatg cgcaggtcca
     2221 tctcagccca gggtgacagg gtaggtcgcc cagatgtcgc gcgcggtggt ggtgtccttg
     2281 cgcggcggcg agccgggctc aggccgtgcc cgatgggcca gcagggtgcg cacgatcatc
     2341 gcgacggtgc cgtagtggtg atcgtcggga tcggtgtccg gcttcggcag cgcgccggcg
     2401 gccagattga gcccaaggca ggcgtgcatg cccaacccga aggaaagtcc gtaggggggc
     2461 acctttgccc cgcggggtcg atcagggttg aaccgctccg catccgcccc gaatgctgcc
     2521 gggtcgcggt ttgccttctg caggtcgacg gtcacccggg tgtccgcctg aacgtccttg
     2581 cctgccacat ggaccgggca gagcgcgcgg cgggcggcaa tggggctcga gggatgcagg
     2641 cggatggatt cgtggacggc gcgctgcacg aacagcgggt cggtctcgaa gcgcgaccag
     2701 tcttccgggt ggctggcgca ccacgtgaag atctcgtgca tgacgtggcc tagggtgtga
     2761 atcgaggtat gggcgcctgc cagcgcgaag aaagccatct cccttacccg cacgtcgtcg
     2821 gccagctgca cggtgtcctg gttcttcagg agcaccgtga ggatgtcccg gggcaggtcc
     2881 tcctcggaca gatcgcctcg gttcacgctg gcgatgagcg cctggcgacg agccaccgaa
     2941 gggtcaacgt agtcgcgctg aaaatccgcg agcgcgtcca gcgcggcacg gcgaatcggt
     3001 tcgcgatcct cggtagagtg ggcgagggtg gccgtgcggc cgaacaacct cagcatcttt
     3061 atgagctgct cggtttcctc cggcgacttc ttcggccggt cgatgccggt gaagtccgcg
     3121 gtgaggttga ccatgacgcg gtagccgaag tccacgatgt ccatctttcc ggcggcgagg
     3181 aagggggcga tggtctcctc gagggtgcgc ggaaaaacct cgtgctcgta gtagcggaag
     3241 aagtcgcgac ggaagacctg ggtttccacg ttgcgccggg tcctgtgctc gtcgccgtgc
     3301 aggttgacga gcacctcgcg catgatcacg gcgccctcgt cgtagagcgc ctgacgcaga
     3361 tctttctgtt tcagcgccgc gttggcggct tggtacgagt cgacggtgac caggctcatt
     3421 tatgtgcgtc cgcccagaaa agctggcttg aggagtaggc ggagacccgc cagccgggaa
     3481 tgggcttgcg tgcgggcagg gggggcaggt ccagctgcgc accgttcaag aacacccgtt
     3541 cgattttcgt cttgtcgccg aggaccgtca cgtccttgga cgggtcgccg tcgactacca
     3601 ggaggtcggc cagccgcccg gcctccaccg cgccgacgcg atcctgcagg ccgatggcgc
     3661 gaccgcactc cgacgtggcc gcgcgtatcg cctcgagcgg ggtgaaaccc atgtcgcgga
     3721 cgaacacctc catttcccgg tagtgccact cgccgtaagg cgtcagcgag aaaccggttt
     3781 ccgtgccgca cagcacgggc acgccggcct cgtaggcgcg gcggatcatg tcggcgctct
     3841 cggcgatctc ccgccggaag atctctcgca ggttggggtc cgcccccacc agatcgccga
     3901 aatcagcgag gttggcctgg aacgtgaaag tcggcaccag cgggaccttc cgctcgatga
     3961 ccgcctcgag cgcggcgtcg tccatgaacg tggcgtgcag gatcatgtcc atgcccgccc
     4021 ggacggagtc cgcgatgctc ttcgcgttgc ggcagtggcc gaccacgggt atgccgagct
     4081 cgtgggcggt gtcgcagacg gtcttcagct cgtcgaagga ccaggcctga atctcaccgc
     4141 ggtgcttctg ccgcggaacc agccccgtca cgtgaacctt gatccagtcc acgccgagct
     4201 ttatctggcg gcgcacctcg gtgacgatct cgtcgcggct gcgcactacc ttggcgtagc
     4261 cgacgcggcc ctccgccgga atcagattac cggcggtgcc gcccacggac gtcagcagag
     4321 cgttcccgcc ggtcgacatc cgcggcccgg gcacgatgcc cgcctcgatg gcatccctca
     4381 ggtccacgcc gatatcgaag atgcagtccg cgtcgaggaa accggtgacc ccggcacgga
     4441 gcagcttctg cacgttgtac gcggccacca gggcgccgag gccttcccgg cggtggaaga
     4501 acatctcgtc gttcgaatgg gtctcgtcca gcgtcacgtg gcagtgcgcg tcgatgaggc
     4561 ctggcatgac ggtcttgccc gcgacgtcga taacggtcgc cccttccccg tcggctgcgc
     4621 ccaccgccaa gatgagctct tcgtcgatga gcacgtcgcc ggcaaacggc gcgttgccga
     4681 gcccgtcgat gatgttggcg ttcttcagca gtgtcttcac agcgcctgct ccttgatcac
     4741 ccgccagatc ttctcggacg tggcaggcat gtcaagctcc cttaccccga gagcgtcggt
     4801 gattgcgttg atgacggccg gtggcgctcc gatggcgccg gcctcgccgc agcccttgat
     4861 gccgagcgga ttcgaaggcg agggtgtatt cgtgaacccg acccggatat ccggcaggtc
     4921 gtccgcgcgc ggcatgcaat agtccatgaa ggacccggac agcaactggc cgccgtcgtc
     4981 gtagacggtg ttctccagca gcgcctggcc gattccctga gcgaccccgc cgtgcacctg
     5041 gccttccacg atcatcgggt ttaccagcgt gccgaagtcg tccacggcga cgaagtccac
     5101 gatctccacc acgccggtct cacgatcgat ctccacctcg cagacgtgac aaccggcggg
     5161 aaagttgaag ttgggcgggt cgaagtagca accctctttc agccccggct cgatctgtgt
     5221 ggtgtcgaag tgatgggccg tatacgcgtt gagggcgact tccgcgaagg aaaggcgctg
     5281 attcgactgg gggctcgaga actctccgtc agcgaagctc acgctctgcg ttggaacatc
     5341 caggacctgg gcggcgatga gcttggcttt ggcaaccacc ttatcgcagg agatggcgat
     5401 ggcggacagg cccaccggac ccgaac
//
LOCUS       DBNHKV010000330         2226 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1958760, whole genome shotgun sequence.
ACCESSION   DBNHKV010000330 DBNHKV010000000
VERSION     DBNHKV010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1958760"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1590)
                     /locus_tag="AC16PP_10315"
     CDS             complement(<1..1590)
                     /locus_tag="AC16PP_10315"
                     /inference="COORDINATES: protein motif:HMM:NF019353.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR6482062.1"
                     /translation="MATSPDKPYDGGFAELHCISNYSFLRGASHPEELVKRATELGYR
                     ALALTDECTYAGLVKAHVAARELGIKLIVGAEFRLEEGIHLILLAPDRSAYGQLSALI
                     TKLRRRSPKGAYEVCLDDFRWGVDECLALWVAGDGDIETLMAQGRRLSERWRELWIAL
                     ELFREDKDLDKMANALTLSMRLDLPIVASNDVHAHLPARQPLQDVLTAIRLKTTVAEL
                     GCAAHRNGERHLRSLSELVSLYPPEMLEASCQIADRCRFSMDELRYEYPEELVPPHLT
                     PGEYLRQLTLAGAAERWPDGVPADTLALIERELTLIREMRYEYYFLTVQDIVQFARSQ
                     QILCQGRGSAANSAVCYCLHITEVDPSRMHLLFERFVSKERNEPPDIDVDFEHERREE
                     VIQYIYKRYGRDRAALAATLITYRPKSAVRDVGKALGMDLDLVDLLAKSLAWWDRREA
                     LNERFAAAGLDPDSPVVGHFLRFVAEILGFPRHLSQHVGGFVISQGPLAQLVPVENAA
                     MADRTIIQWDKDDLEALGLLKV"
     gene            complement(1574..>2226)
                     /locus_tag="AC16PP_10320"
     CDS             complement(1574..>2226)
                     /locus_tag="AC16PP_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482063.1"
                     /translation="IWTLDDHGSTDTAELKIRFARSQQLREAFLDITRLKLESAELPG
                     DVLGIGLRAERVAPWQAGSRTLFQSQPGQAPADAAGLVDQLCARLGNRACHGIRPVDQ
                     HAPEQAWRRSRPAMNGRGGRRSRSRSGKHGNGRATDGEEALLPAPDERPIWLLSRPRP
                     INRSELKLLKGPERIRTAWWQRGISRDYYVARDRRGAECWAFVDAGNNWYLHGYFA"
BASE COUNT          351 a          713 c          789 g          373 t
ORIGIN      
        1 caccttgagc agccccaggg cctccagatc gtccttgtcc cactgaatga tggttcgatc
       61 cgccatggcc gcgttctcca ccggcaccag ctgagcaagc ggcccctggg agataacgaa
      121 tcctcccacg tgctgggaga ggtgcctggg gaaaccgagg atctccgcga cgaagcggag
      181 aaagtgcccg accacggggc tgtcgggatc gagcccggcg gcggcgaaac gctcgttcaa
      241 cgcctcccgg cggtcccacc aggccaggga tttggccagc aggtccacca gatccaggtc
      301 catgcccagc gccttgccca cgtcccgcac ggcgcttttc gggcggtagg tgatcagggt
      361 cgcggccaga gcggcgcgat cgcgcccgta acgcttgtag atgtactgga tgacctcctc
      421 gcggcgctcg tgctcgaaat ccacgtcgat gtccggcggc tcgttgcgct ccttggagac
      481 gaagcgctcg aacagcaggt gcatgcggga gggatccacc tcggtgatgt gcaggcagta
      541 gcagacggcg gagttggccg ccgagccgcg gccctggcag aggatctgct ggctgcgggc
      601 aaactgcacg atgtcctgca ccgtgaggaa gtagtactcg tagcgcatct cgcggatgag
      661 tgtgagctcc cgctcgatga gcgcgagggt gtctgcgggg acgccgtccg gccagcgttc
      721 ggccgcaccg gcaagggtga gctgccgcaa gtactcaccc ggagtcaggt gggggggcac
      781 cagctcttcg ggatactcgt agcgaagctc gtccatggag aagcggcagc ggtcggcgat
      841 ctggcaggac gcctcgagca tttccggtgg gtagagagag acgagctcgc tcagggagcg
      901 caggtggcgc tcgccgttgc gatgggccgc gcagccgagc tcggcgaccg tggtcttcag
      961 gcgaatggcg gtgaggacgt cctgcagggg ctgccgggcg gggaggtgcg cgtggacgtc
     1021 gttgctggcc acgatgggca ggtccaggcg catggacagg gtcagggcgt tggccatctt
     1081 gtcgaggtcc ttatcctccc ggaagagttc gagagcaatc cagagctcgc gccagcgttc
     1141 gctgagccgt ctgccctggg ccatgagggt ttcgatgtcg ccgtcgccgg ctacccacag
     1201 cgcaaggcat tcatccacgc cccagcggaa gtcgtccagg cagacctcgt aggcgccctt
     1261 gggggagcgt ctgcgcagct tggtgatcag cgcggagagc tgcccgtagg cgctgcggtc
     1321 cggcgccagc aggatgaggt gaatgccttc ctcgaggcgg aactccgcgc ccacgatgag
     1381 cttgatgccg agctcgcgcg ccgctacgtg agccttgacg agtcccgcat aggtgcactc
     1441 gtcggtcagc gcaagcgcgc ggtagccgag ctcggtggcc cgtttgacga gctcctccgg
     1501 atgggaggct ccgcgcagaa agctgtagtt gctgatgcag tgcagctccg cgaagccacc
     1561 gtcgtacggc ttgtcaggcg aagtagccat gcaggtacca gttgttcccc gcgtcgacga
     1621 aggcccagca ttcagcgccc cggcggtccc gggcgacgta gtaatcccgg ctgatgccgc
     1681 gctgccacca ggcagtgcgg atacgctcgg ggcccttgag cagtttcagc tcgctcctgt
     1741 tgatcgggcg cggccggctg agcagccaga tcggccgctc gtcgggcgcg ggcaggagcg
     1801 cttcttcccc gtccgtcgcc ctgccgttgc catgcttgcc gctgcgactg cgactgcgcc
     1861 tgcccccgcg cccgttcatg gcaggccgtg aacgacgcca ggcctgctcc ggcgcgtgct
     1921 ggtccaccgg gcggatgccg tggcaggccc ggttgcccag ccgggcgcaa agctgatcga
     1981 cgagcccggc agcgtctgcg ggggcctgcc cgggctggga ttgaaaaagt gtccggctgc
     2041 cggcctgcca gggcgccacg cgttcggcac gcaggccgat gcccagcacg tcgccgggca
     2101 attccgcgct ttcgagcttc agccgggtga tgtcgagaaa agcctctcgc agctgctggg
     2161 acctggcgaa gcggattttc agctcggcgg tgtctgtcga tccatgatcg tcgagcgtcc
     2221 agatga
//
LOCUS       DBNHKV010000331        24037 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1749173, whole genome shotgun sequence.
ACCESSION   DBNHKV010000331 DBNHKV010000000
VERSION     DBNHKV010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 24037)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24037)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24037
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1749173"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="AC16PP_10325"
     CDS             <1..516
                     /locus_tag="AC16PP_10325"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6482064.1"
                     /translation="PDKGADIKCNPAIKGAADQEALLRAVAEDRIDVIATDHAPHTRE
                     EKDREYLRAPAGLPLVQHALSSLVEQVHRGVFDIETVVRKTAHAPALLFGVKERGFIR
                     EGYHADLTVIDGDALVDGGEIHSKCGWSPFSGIDLHGRVRATWVNGVLRYRDGRILDG
                     PSGIALEFERP"
     gene            complement(519..1457)
                     /locus_tag="AC16PP_10330"
     CDS             complement(519..1457)
                     /locus_tag="AC16PP_10330"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR6482065.1"
                     /translation="MQFKNRVTEMLGVEIPVVQAPMGWIARSQLASAVSNAGAMGIIE
                     TSSGELDAVREEIRRMRELTDKPFGVNIAQAFVRDPNIVQFVIDQGVKFVTTSAGNPE
                     RFTDELKSAGLTVFHVVPSLSAALKAVACGVDGLVVEGGEGGGFKNPREVSTMVLLPL
                     VRSKVDVPIIAAGGFVDGASMAAAFALGAEGVQLGTRMVSALESPVHQNWKDAIVNAG
                     ETDTVFLNRKHSPALRALRTEKTTRLENEPDTNAMSEFGSALDLYFGGDMEASIALSG
                     QVCGRIDAVRPVAEIIAELKDEFFGTIARLDEQYLS"
     gene            1664..2950
                     /locus_tag="AC16PP_10335"
     CDS             1664..2950
                     /locus_tag="AC16PP_10335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6482066.1"
                     /translation="MQARPPKPFKARDLTADRATAMRFTAVLGLQMGQIFPGSFIALF
                     LPVIYREQGLSLEMYWVFTLPAIPTWIRPLWAPFVDRTGSRIIGMRKTWFIPCTVFGA
                     LAYLSLTQFAPTLENLAVIITILVIKTTFMTTQDIAIDGYMVENIRDEERPVGAAVLD
                     IGRNVARFIAWAGIAWVYTEHGWSAAVTVASALLILFSAPGIIRREPPRPSQFDHVRP
                     SLKNLLRRRDSYYVYPLCFGIALMGALVQSLYPTFLSDLGFSLGEVAAVIAPAILIGS
                     VLGASVTSWYLGRFGYKATVLTAAFAVVLAMLPIMWMGSLEEPTFVVVFAVTLNAIAL
                     PSVLDVSFQAARLKWASKSQAATDYTSQIVTMAAGGGLAVAVGGFMAEYLGWFYYFLV
                     AGTLVSGACFVLYYLFDTIEDIVEERDRTEAEGGNL"
     gene            3010..3807
                     /locus_tag="AC16PP_10340"
     CDS             3010..3807
                     /locus_tag="AC16PP_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6482067.1"
                     /translation="MALVDYEIHGHIAVITLNRPAARNAINPEVAVRLADAWESVRDD
                     DQVRVAILTGTGSAFCAGADLGQLIPMVSGTRKPENEWDERVASEPGITNRALLRNFD
                     TEKPIIAAINGHAIAGGMEIVQGTDIRVSVAEAKFGVQEVKWAIFPAGGSTVRLPVQL
                     PYARAMELLLTGDLISAEEALNIGFLNYVTDDIMDKAMEIAEKIAANGPLAVKAIRRS
                     VKECLGKPEREALKIESEISGPVFRTEDAKEGPRAFMEKRAPEYHGR"
     gene            complement(4227..4469)
                     /locus_tag="AC16PP_10345"
     CDS             complement(4227..4469)
                     /locus_tag="AC16PP_10345"
                     /inference="COORDINATES: protein motif:HMM:NF042903.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HIR6482068.1"
                     /translation="MPQEKRKRRATAAPRTDARPPAMANRPPSRQGKKPVTAYVDKQV
                     HKQLRSLGLELEKSNQEMILEALNDYFERHRLSTKG"
     gene            complement(4494..5255)
                     /locus_tag="AC16PP_10350"
     CDS             complement(4494..5255)
                     /locus_tag="AC16PP_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HIR6482069.1"
                     /translation="MHVVCLLSLKGGSGKSTVVQSLSVCAQQHGHRTLIVELDPQGTL
                     KNWSKRRESEQPRVVQTLPQSVGDVLEEAKAEGVHWVFLDTPGHHVASASAAAELADL
                     ILIPCKIQSVKDFDAVLPSLAEARRADKPAYVLMNQVPPNSPRLIRRRQLQIQKHYDI
                     AVLSRFLCRRADFEYCDERGLSAAELNPSGPAAEEIDRLYALLQSIFIMRSLKVDKQA
                     LVPAPGTSVGPVSRSSEVEPRPLERGQARTEELTQ"
     gene            complement(5417..6007)
                     /locus_tag="AC16PP_10355"
     CDS             complement(5417..6007)
                     /locus_tag="AC16PP_10355"
                     /inference="COORDINATES: protein motif:HMM:NF039725.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6316 family protein"
                     /protein_id="HIR6482070.1"
                     /translation="MPGKLPQWFRADEVYAYSGQWYFGSREGLHIGPYADREVAKAKG
                     TQVAKRLRAMDEAHQLKFVRKVLDREWDSVSKLIPTTGIGDAAAGSEPPPKEAVRHGE
                     GYRNWYRSGRYFQADGAWFFSTREGIDVGPFETEVQAKKHERRLVALLQRTQSAEEAF
                     QIIHEYKYRPSARSVGRVWANVTPASRTRPTKPRWA"
     gene            complement(6183..7361)
                     /locus_tag="AC16PP_10360"
     CDS             complement(6183..7361)
                     /locus_tag="AC16PP_10360"
                     /inference="COORDINATES: protein motif:HMM:NF014954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6482071.1"
                     /translation="MSGRGEGPLPIPETPAALTPEWLTEALRRGGILDDQKVVDIEAE
                     VLGEGVGFLGDLLRLSLSYDRPGNLPDSLIAKLPKLDNRAMGEMLGAYERENCFYMEM
                     AGSLPLSTPRMYYGDFDRDRASEKQEDILRLFDRMPRFLLSFLPALGLRIAAAKKRRY
                     ILLLEDLSDAEPGDQVAGVSVRRCERALRAIARAHAACWGAELVDAFWLLPHDIDARM
                     RHSMFKGSRSTFSDFFGPELEARLEPYLRRLPDQGLDLIRRLNQAPTTLLHCDLRLDN
                     LFFREDEVIVIDWQLVRRGPAVYDVAYFLSSALDEAAGRAVVEGLLEAYLEELQAGGV
                     DGYTMDELLRDYRIALRVVLMTLSTVDQVELGDGRGIDLMRGWISRLHGRLAEMGEPG
                     "
     gene            complement(7358..8083)
                     /locus_tag="AC16PP_10365"
     CDS             complement(7358..8083)
                     /locus_tag="AC16PP_10365"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6482072.1"
                     /translation="MTTRIRGVFFDLGGTLFSYRNMGRSTGPALLEAAARMGITGDPR
                     SIGKAYGLASREVNGAYAERDFYLHRDLFRDTFVRFVEQMEGQFDEAAYAWFRARQHE
                     LVLSGLELKPDCTETLSHLKSEGLYLSIVSNIDDDMLDPLVEREGLHDYLHHWTSSEA
                     AESCKPHRRFFEISLEKSGLAPGEVLFVGDSPEHDVQGAYEVGMNTVLIREEGLEPPL
                     QVGRQTVEPDYTINSLAEIRGLI"
     gene            complement(8144..9790)
                     /locus_tag="AC16PP_10370"
     CDS             complement(8144..9790)
                     /locus_tag="AC16PP_10370"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HIR6482073.1"
                     /translation="MQHTPLLMSNILDRGAKVAPDVEIVTATENGTRRQTYAEIRDRA
                     HQLAHALRDAGIGVGDRVATFMWNGSRHLEAYHAIGGMGAVLHTLNIRLSAKDLEYII
                     NHAEDRVIIADADVLPLLEPLKGRIPSVERIVVATEDGFEGWSTEVAEAVDYEEFIAG
                     KPTRFDWPRIDENSALGLCYTSGTTGNPKGVEYEHRSQYLHTLTQCMTDSMGLSATDC
                     VLGIVPMFHAMGWGMPWSCLMLGCKQVMPHRFMDTSRLLDLLVSEGVTMSAGVPTIWQ
                     GIKALVEADPERYDLSRLARMACGGSAPPPSLIRWYWDTLGVEMIQGWGMTETSPLAT
                     LSRRVMKRSHLELSEDDQFRNVAKAGQLMPGLELDIFDEDFNRLPHDGETVGEILIRG
                     PWICSEYYHDPQPEKFHDGWLITGDVGKIDSEEYLIISDRSKDLVKSGGEWISSVDLE
                     NHIVSLDGVEQACVVAQPHPKWDERPVALVVLGDGADVSLETVLAHCSQAFAKWQLPD
                     DVLYVDSIPLTSTGKMDKKIVRADLEAQGYVLPDLREKAG"
     gene            complement(9863..11440)
                     /locus_tag="AC16PP_10375"
     CDS             complement(9863..11440)
                     /locus_tag="AC16PP_10375"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0032787 - monocarboxylic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6482074.1"
                     /translation="MTSSMMRLTQGIHRALSLYPRRRAVVSAEGEITWEQFADRVARL
                     AGALRELGVGPGDRVAMLALNSPRYVEYYFAVLWAGGVLAPVNCRWAAPEKAHCLNDS
                     GAKVLLADAAHLPEALELLETCGSVSHLVSCGEVEGSGTAALGYESLVSASLPVEDAE
                     RGGADLAALFYTGGTTGRAKGVMLSHDNFIANSMTALVNLGISEHSVHLHVAPLFHLA
                     GGSRLFTLTVAGGTHAVIPRFDAAEFLGAIERFGVTVTVIVPTMLNTLLQHPNFEAFD
                     LSSLDLLCYGASPMPESLLREAMERLPGARFLQSYGMTELSPVATMLEPQFHTFEGPL
                     AGKIRSAGRAVFNADVAIMSPDDLPLASGEVGEICVRGPMVMQGYWGQPELTAQALRG
                     GWMHTGDAGYLDDDGFLFLVDRVKDMIITGGENVYSAAVENVLYLHPGVHECAVIGVP
                     SDAWGEAVHAIIVPRGGARLDEAAVIEHCRAYLAGYECPKTVEFVDAELPKSGAGKIL
                     KAELRKTYWQGQARNIH"
     gene            complement(11496..12671)
                     /locus_tag="AC16PP_10380"
     CDS             complement(11496..12671)
                     /locus_tag="AC16PP_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6482075.1"
                     /translation="MSGPLTGVRVLDLTSMVSGPVATMLLGDQGADVIKVEPLTGDLV
                     RYMGPNRDGLTSGFISSNRNKRSIAVDLKQEAGMDVVKRLAATSDVFVQNFRPGAIER
                     MGLGEDVVREIRPDIVYVSISGFGETGPYAHKRVYDPVIQALSGLAAIQRDRETGRPK
                     MVRTIIPDKTTALTAAQAITAALFARERSGEGQHVKLAMLDTMVAYLWPEGMAGFTFI
                     GKEVKAARAQFAQDLIFATTDGFITAGAVSDAEWQGMCRALEREEWIDDERFNTPNGR
                     IVNVQERLRLTAEVLETRSSAEWLARLDAEGVPCAPVLDRHEVFEHEQVRINEMVEEL
                     DHPVAGRIRQPRPAARFDRTPAGIHRHAPMLGEHTDELLQEMGLDADALRAAGVIGG"
     gene            complement(12681..14294)
                     /locus_tag="AC16PP_10385"
     CDS             complement(12681..14294)
                     /locus_tag="AC16PP_10385"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6482076.1"
                     /translation="MDFHFASAWEAIADTVPDRTALICEGVTRTWADYDGRATRVAGL
                     LQAHGLGADTKVGLYLHNCNEYLEAQYGVFKLSGCPINVNYRYKAEELVYLLDNADAE
                     AVFYQGCYAPRIEEIRDQLPRVKLYVQVDDGTEDLLAGSVDYESSLDAAEPMARQKRD
                     PASVYMLYTGGTTGMPKGVMYPGGEFCYLLTAMGAGGRGLVPPQNLEALREYVAGIED
                     PPVTVVACPLMHGTGMWLGSLAPLLTGGTVITTPQLGLDPDLIWTLVGSHRATDLVVV
                     GDAFARPLLAALDEAERRGEPYDLSSLRQIVSSGVMWSREVKEGLLRHRDMMLVDAMG
                     STEGSMGSSVSSRASGPTTAKFQLAEGVKVFTDEGREVEPGSGEIGKLATSGFVPLGY
                     YKDPVKSAETFREVDGVRYSFPGDYATVEADGSITLLGRGSVCINTAGEKVFPEEVEE
                     AVKRHPAVVDCLVVGIPDERFGERVVAVASFHDDQDVSEPELIEFARDHLAGYKLPKQ
                     VLRVPQVQRAPNGKADYKWAKSTALAELT"
     gene            14568..14783
                     /locus_tag="AC16PP_10390"
     CDS             14568..14783
                     /locus_tag="AC16PP_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482077.1"
                     /translation="MIIKDNAPAGPIARLSRITYPRASAANLMLLSVVIAAIGAIAYI
                     LHPDAAQPAPNTFHGAALLVSNWLLCG"
     gene            14811..15023
                     /locus_tag="AC16PP_10395"
     CDS             14811..15023
                     /locus_tag="AC16PP_10395"
                     /inference="COORDINATES: protein motif:HMM:NF015957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR6482078.1"
                     /translation="MTNSNLNNVRRRLRLDPRNGWIAGACAGFANRLQIDPAIVRVGV
                     VIAAMFFPKTVIACYLLAWLVLDEAR"
     gene            complement(15048..15929)
                     /locus_tag="AC16PP_10400"
     CDS             complement(15048..15929)
                     /locus_tag="AC16PP_10400"
                     /inference="COORDINATES: protein motif:HMM:NF039714.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HIR6482079.1"
                     /translation="MVVRLFALLAVLVLGSGCSVQFAYNNLDRLVRWGVNDYVNMNDA
                     QRNLFNEEFARLHYWHRTEHMPLYADFFEAVPTTLSDGVDAQELLALENTMMGWAKEI
                     VETGAPLAVAIMQTMTDEQVAGLPARLEESNREIEKTELGKSVEASQARWREEVEDIF
                     TRFAGRLTPAQQEYLVAQSVRYIPERVLWAEYRRRWQRDLMKLLQARSEPAFADRFAE
                     LAANREAYFGAELTAIFDNNENLGRETAAWLLNHMAERQSVRMYTRFVELAIDFRELA
                     DDAPNWLPPEEPCQITC"
     gene            complement(15934..16635)
                     /locus_tag="AC16PP_10405"
     CDS             complement(15934..16635)
                     /locus_tag="AC16PP_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase-related protein"
                     /protein_id="HIR6482080.1"
                     /translation="MRIGILQTGAVLEDIQPRFGDYPAMFVDLLSGDAHRRPSFSTYR
                     VREGELPEPAACDAYVITGSRHSVYEDHPWIVELAEFVGNTMAGGQKIVGICFGHQLI
                     AHYFGGRTEPASVGWGVGVHGVEIVSRERWMDPHQGSVSMLSSHKDQVVALPRGARRV
                     ASSAFCPNAAFAIGDVAMTWQGHPEFEKDYAETLMRRREALLGPETLAAGLASLDNEI
                     DRALLARWILNFIYD"
     gene            complement(16653..18029)
                     /locus_tag="AC16PP_10410"
     CDS             complement(16653..18029)
                     /locus_tag="AC16PP_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR6482081.1"
                     /translation="MNDGMELLDQKLARYRERGVSRVKLGLTDVDGVIRGKYVSLSKF
                     ASLMEKGGGFCDCVFGWDVHDQLYDAGTYTGWHTGFPDAQYRLVADSERWLSEEGCPF
                     FLGEFVAGDGGPHPLCPRTLLKTVLGRLGDRGLTLKSGFEYEFFVFAETPHSVRDKGY
                     RNLRPLTPGNFGYSLLRASGEADKFSGLMDFSQALDCDLEGLHCETGPGVWEAALQTA
                     LGEEAADRANIFKTFAKVYFNRLDCMASFMAKWSMDYPGQSGHFHLSLLDADGRNVFF
                     DDGQPSGMSDLQRQAVAGLERYLPEYLALIAPTINSYTRLVKGAWAPTASTWGIDNRT
                     VAIRVINGSESHQHIECRVPGADSNPYLVAAAVCAAALGGIEQALLPGDPVVGNSYDV
                     EDSLPEELRFPRDLAAAADRLEASDHARAMLGEAFVDHYVMSRRWECEEYRKNLNSWQ
                     LERYFEII"
     gene            complement(18436..18687)
                     /locus_tag="AC16PP_10415"
     CDS             complement(18436..18687)
                     /locus_tag="AC16PP_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482082.1"
                     /translation="MRVFLIALMACLVTLSFVGHGHAADAAAAAPPLVSTGAEAAHQV
                     AAEEGRVDWHLDTAIFTDISPIDTPEVAIKLPEDQQEEG"
     gene            complement(18707..20623)
                     /locus_tag="AC16PP_10420"
     CDS             complement(18707..20623)
                     /locus_tag="AC16PP_10420"
                     /inference="COORDINATES: protein motif:HMM:NF015803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HIR6482083.1"
                     /translation="MKFSPETVRLRRVAKAYAAGEFSRSEYRATRREVIANFRADASS
                     DDDTQRRDAVTVGSGAAVDPDQPRGRSATWLVMVALTMMVLGLVALEARAGTVIGAVS
                     ERSSDPMSSPRLAVRSLRVRAFQPYPGVLESDLNALIDDALDEIRRRNAEGDHGFTRT
                     ELDEVGRFLSALGAHRDGGALSATDAEDLMALIEQQKARRGISVLELEEIAGRVQAYY
                     RDAGYFLAVAYLPAQQVEHGIVEVGVLPGVLGDVVVTGVDQKELASRFEEVVGEPVTE
                     ESITRRVYLLNQVPGLKAQAAFEPGSEVGETRLNVNVVEQRKWSARVIADNHGDDATG
                     KERLSVVGGWFNPRGVGDVLEVGLLQTVNPTNQTYGFIDYRTPIGGVRQAHVRASTNS
                     FTASDGVELDGSGALFDVGTELALRRSRASSLTARLGVSHHRFSWATAQDQAATFLSA
                     GFDGHRLWDDRRVAAGFGAVADAGRFGDGTFAGQDATFWRLGLNAYAWRALNVPGLPG
                     EQKLAARLLGQLSGSQLPSSRRLSLGGFGRVRGFERDAFLADRGALLSLELRTPVPLG
                     EMALFADTSYGEGLNDLTPAWGHLTSLGVGWDADLLPRLTSRVSLALPVAAKGSGDMD
                     DKGFRIFWQLQYEH"
     gene            complement(20629..23142)
                     /locus_tag="AC16PP_10425"
     CDS             complement(20629..23142)
                     /locus_tag="AC16PP_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HIR6482084.1"
                     /translation="MSRPAHQDATVIRADPSLSVSFAGEGVGETNAPRILKQRFVLEE
                     KLGSGGMGTVFRAKDLRKVEARDRQPYLAIKVLNDNFRAHPEAFIALQREAAKSQAVS
                     HANIVSIYDYDKDGDVPFITMELLEGQELADLLRAYPNGLPDPMAWEVIRGICAGLGH
                     AHSTGVVHADFKPGNVFVSPRNAAKVLDFGIARAVRLNQIYGEETVIDPVRLGALTPA
                     YASREMLQGDNPEPRDDIYALGVVIYLILTGHHPYGRTPADEAAREGLAPDRPRRLTR
                     RQWRVLEKCLRFNRHERPATIDEVEHYLLKPAPWRSRTALAAAAAFALSLGINYLVGD
                     AELTEVREEVRQSTLVDAQTARVQAMLSEPDFEPGWEDRIASELDRLNVLDESGASGA
                     RMSAQIIALLTQRIVATRDLGNAFELYRSATRFGEPERAGRVLHDRLLARVFALLESP
                     QPEQRWFDSVRRELARLASTFPESKEIEPIRLEVGDAVEAQLLRSIDRGDFPAAKAAL
                     AELEPLRFDVAAVERLARAVEDAEFGYAAAEQLRAVEENAERFEAAIADVLDTSCLRL
                     DLGPVAALFDAWVAEDAGLGSPGRQMIGAKVAGCVAQLGEHDLARAAALADSARTRFG
                     ELPQLAEVRLDPCSARYLVGAGAQAGRSGYCADRLVGDLVGPRLVVVPNGEARFAITR
                     HEISRGQLNAYCAYSGACGVADADQLPATGVDVVVAQGYARWLSEITGFTYRLPTLTE
                     WRLAAQGAPDASRNCRVEVDGVERGLALVPASSGQAGEFGLINALGNAQEWVLDGGKL
                     RVAGGAFDDAIGDCVPTTVRDHQGRADAVTGFRLVREIS"
     gene            complement(23139..23861)
                     /locus_tag="AC16PP_10430"
     CDS             complement(23139..23861)
                     /locus_tag="AC16PP_10430"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR6482085.1"
                     /translation="MELSWQSAEACHCGKRRASNDDAVVSRPSARLWAVADGMGGHKA
                     GDVASGAIADALGRLTLNGNLADDVDAVDDALGEVNEQLREHARETCPGATVGSTVVA
                     MLALAGVGVALWAGDSRLYRLRGRRLEQITRDHNPVSDLLDSGAVSESEAVAADTNIV
                     TRAVGGHSELFLDVAVFDIDPQDTYLLCSDGLYRELNTADLAHGLQGESPAEIANGLL
                     SRCLETAARDNVSLIVARPDVT"
BASE COUNT         4302 a         8117 c         7477 g         4141 t
ORIGIN      
        1 cccgacaagg gtgccgacat caagtgcaac cctgccatca agggcgccgc tgaccaggaa
       61 gccctcctgc gcgcggtggc ggaagaccgc atcgacgtga tcgccaccga tcacgccccc
      121 cacacccggg aggagaagga tcgcgagtac ctgcgcgccc cggccgggct gccgctggtg
      181 cagcacgccc tttcatcgct ggtggagcag gtccaccgcg gcgtgttcga catcgagacc
      241 gtggtccgca aaacggccca cgcaccggcg ctgctcttcg gcgtcaagga gagaggcttc
      301 atccgcgagg gataccacgc cgacctgacc gtgatcgacg gcgatgccct ggtggatggc
      361 ggggagatcc actccaagtg cggctggagc ccgttcagcg gcatcgatct gcacggcagg
      421 gtccgggcaa cctgggtgaa cggcgtgctc cgttaccgcg acggccggat cctcgacggc
      481 ccgagcggca tcgcgttgga gttcgagcgg ccctgaactc agctcagata ctgctcgtcc
      541 aggcgcgcga tggtcccgaa gaactcgtct ttcagctcgg cgatgatctc cgccaccggg
      601 cgaacggcgt cgatgcggcc gcaaacctga cccgacagag cgatgctcgc ttccatgtcg
      661 ccaccgaaat agagatccag cgcggagccg aactcgctca tggcgttggt gtccggttcg
      721 ttctccagcc gcgtcgtctt ctcggtgcgc agcgctctca gcgcgggcga gtgcttgcgg
      781 ttgagaaaca ccgtgtccgt ttcgccggca ttcacgatgg cgtctttcca gttctgatgc
      841 acgggtgact ccagcgcgga caccatccgc gtgccgagct gaacgccctc cgcgccgagc
      901 gcgaacgctg ccgccatgga cgcgccgtcc acgaacccgc ccgcggcgat gatgggaaca
      961 tccaccttcg agcgaaccag gggcagcaga accatggtgg agacctcgcg tgggttcttg
     1021 aagccgccgc cctcgccgcc ttccaccacc agtccatcca cgccgcaggc cacggccttc
     1081 aacgcagcgc tgaggctggg aaccacgtga aagacggtga gcccggcgct cttgagctca
     1141 tccgtgaacc gctccggatt gccggcagac gtcgtcacga acttgacgcc ctggtcgatg
     1201 acgaactgaa cgatgttggg gtcgcgtacg aacgcctggg cgatgttgac accgaacggc
     1261 ttgtcggtga gctcgcgcat cctgcggatt tcctcccgaa cggcgtcgag ctccccggag
     1321 gacgtttcaa tgatgcccat ggcgcccgcg ttcgacacgg cggaggcgag ctgcgagcgg
     1381 gcaatccatc ccatcggagc ctgcacgacg ggtatctcga cccccagcat ctcggttact
     1441 cggttcttga actgcatgac gccccccggg gaaatcgggg agcctacctc agctgccggt
     1501 ggacgtgtag tggcgcaggc cgaatcgtca gaggaagacg gccagcccga aatagaggaa
     1561 cccagcaggg tgcgggtggc cggaaagggc cgcggattct actggagcgt cgatcgcaga
     1621 aacaacccgt gtgccatccg ggcgccgccg ctaaagtggc cggatgcagg cccgcccacc
     1681 caagccgttc aaagcgcgcg acctgaccgc cgatcgggcg acggccatgc gcttcaccgc
     1741 ggtgctcggc cttcagatgg gtcagatctt tccgggctcg ttcatcgccc tgtttctgcc
     1801 ggtgatctac cgcgagcagg ggctgtcgct ggagatgtac tgggtcttca cgctccccgc
     1861 catcccgacc tggatacggc cgctctgggc gccgttcgtc gaccggacgg gcagccgcat
     1921 catcggcatg cgaaaaacct ggttcattcc ctgcacggtc ttcggcgctc ttgcgtatct
     1981 cagcctgacc caattcgcgc caaccctcga aaatctcgcc gtgatcatca cgattctggt
     2041 gatcaagacc acgttcatga ccacccagga catcgccata gacggctaca tggtcgagaa
     2101 catccgggac gaggagcggc cggtgggcgc cgccgtgctg gacatcggcc gcaacgtggc
     2161 caggttcatc gcgtgggccg gcatcgcctg ggtctacacg gagcatggct ggtcggccgc
     2221 ggtcaccgtg gcctcggctc tgctgatcct gttctccgca ccaggaatca ttcgcaggga
     2281 accgccgagg ccgtcccagt tcgaccatgt ccggccgagc ctcaagaacc tgcttcgccg
     2341 tcgcgacagc tactacgtct accctctctg ctttggaatc gctctgatgg gcgcgctggt
     2401 gcagtcgctg tacccaacgt ttctttccga tctgggcttc agcctcggcg aggttgccgc
     2461 cgtgatcgcc ccggccatcc tgatcggttc ggttctgggg gcgtcggtca ccagctggta
     2521 tctgggacga ttcgggtaca aggcaacggt tctcacggcg gcgttcgcgg tggttctggc
     2581 gatgctgccc atcatgtgga tgggatcgct ggaggaaccg acattcgtcg tggtcttcgc
     2641 ggtgaccctc aacgccattg ctctgccttc ggtcctcgat gtgagctttc aggctgcgcg
     2701 cctgaagtgg gcgtccaaat ctcaagcggc caccgactac acctcgcaga tcgtcaccat
     2761 ggcggcgggc ggggggctgg cggtcgccgt gggcggtttc atggcggagt acctgggatg
     2821 gttctactac ttcctggtcg cgggaaccct ggtgagcggc gcctgcttcg tgctctacta
     2881 tctcttcgac acgatcgaag acatcgtcga ggagcgcgac cggacggaag ccgagggcgg
     2941 taacctctga cccttcacga gcagagccaa gcccgcgtca caacaaatgc agaggaaggg
     3001 gaggatccaa tggcactcgt agactacgaa attcacggac acatcgcggt tatcactttg
     3061 aaccggccgg cagcccgcaa cgcgatcaat ccggaggtgg ccgtacgcct ggcagatgcc
     3121 tgggaatcgg tacgtgacga cgaccaggtc cgcgtcgcca tcctgacggg cacgggcagc
     3181 gcgttctgcg ccggggccga tctgggccag ctcatcccaa tggtctccgg aacgcgcaag
     3241 cccgagaacg agtgggacga gcgtgtggct tccgagccgg gcatcaccaa ccgggcgctg
     3301 ctgcgcaact tcgataccga gaagccgatc attgcggcaa tcaacggcca cgccatcgca
     3361 ggtggcatgg agatcgtcca gggcacggac atccgcgtct ccgtggcgga ggcgaagttc
     3421 ggcgttcagg aggtcaagtg ggccatattc cccgccggcg gatccacggt gcggctgccg
     3481 gttcagctgc cctacgcgcg cgctatggag ctgctgctga ccggtgatct gatctctgcc
     3541 gaggaagccc tgaacatcgg gttcctcaac tacgttaccg acgacatcat ggacaaggcg
     3601 atggaaatcg ccgagaagat cgcggcgaac ggaccactgg cagtgaaggc gatccgacgt
     3661 tccgtgaagg agtgcttggg caagcccgag cgcgaggctc taaagatcga atcggagata
     3721 tccggacccg tgttccgtac cgaggatgcc aaggaagggc ctcgagcttt catggagaag
     3781 cgggcgcccg aataccacgg acgctgacaa cgacgggcta agcgaagcgc tccccgacgg
     3841 gtctcggcgc gcggcgaagc tcactgctgc cgcccgccag cctgcgctct cggcttttgc
     3901 gcatctgagc agctgccgga ggcgcccaga ctgctcgcat ctcgtcgtcc tgccagacgg
     3961 cgaatctgga acctatgcgc cttcctgccc gaatcgcatc ggtcagcctc tcgaagacag
     4021 ggccgtccga tgagcaggtc aaggctggaa accggagcat aaaagccaag cgacacctcc
     4081 ttactgagga gacacagcat actctctcga ggccttcacg atttcgaatc ccgtgtgcag
     4141 atcgtattcg catcgtaatg ccaacgtaac ggaacgttcc cgctggcggc gaccccgtca
     4201 gcggaactcg tggacgctgt gatgcctcag cctttagtgc tcagccggtg ccgttcgaag
     4261 tagtcgttca aggcttccag gatcatctcc tgattggact tctcaagctc cagacccagc
     4321 gacctcaact gcttgtgtac ctgcttgtcg acgtaggcgg tcacgggctt cttgccctgc
     4381 ctgctcggcg gccggttggc catggccggt gggcgggcgt ccgtgcgcgg agcagccgtg
     4441 gcccgccgct tgcgcttctc ctgcggcatg tgtccttccc tgtgtgttgt tgtttactgg
     4501 gtgagttctt cggttcttgc ctgcccgcgc tccagcggcc ggggctctac ctcgctcgag
     4561 cggctgaccg gcccaaccga tgtgcctggt gcgggaacca gcgcctgctt gtccaccttc
     4621 aagctgcgca tgatgaagat cgactgcagc agcgcgtaca accggtcaat ctcttccgca
     4681 gccgggccgg aggggttgag ctccgcagcc gacagtccgc gctcgtcgca gtattcgaag
     4741 tcggctctcc ggcacaggaa tcgcgacaac acggcaatgt cgtagtgttt ctgtatctgc
     4801 agctggcgac gacggatcag ccgcggcgag ttgggcggca cctgattcat caggacgtag
     4861 gccggcttgt cagcccggcg cgcttcggcg aggctcggca gcacggcatc gaagtccttg
     4921 accgactgta tcttgcaggg aatcagaatc agatctgcaa gctccgctgc ggcggatgca
     4981 gacgcgacat ggtgaccggg cgtgtccagg aacacccagt gcacaccttc cgctttagcc
     5041 tcctccagaa cgtcgcccac cgactgcggc aacgtctgaa cgacgcgggg ctgttcggac
     5101 tcgcggcgtt tggaccagtt cttgagagtt ccctggggat ccagctcgac gatcagggtg
     5161 cggtgcccgt gctgctgcgc gcaaaccgac agcgactgca ccaccgtgga cttccctgat
     5221 cctcctttga ggctcagcag gcagacaaca tgcacaatca actccgtttg tcgacatgta
     5281 tacatgtcac cgtaacacac gttccagccg cttgttaacc cacgtttttg taaggtaaaa
     5341 gtacttatcg cgctggcgac acggcaacca tgcgtaaaag aaacggagtt aaagagcggg
     5401 aagaccgggc cgggatctat gcccagcgcg gcttcgtcgg cctggttcga ctcgcggggg
     5461 tgacgttcgc ccagaccctg cccaccgacc gcgccgaggg acgatacttg tattcgtgaa
     5521 tgatctgaaa cgcctcttcc gcggattggg ttctctgcag aagcgcaacc agccggcgtt
     5581 catgcttctt tgcctgaact tccgtttcga agggaccgac gtcgataccc tcgcgggtag
     5641 agaagaacca cgcgccgtcg gcctggaaat agcggccgga ccgataccag tttcgatagc
     5701 cttcgccgtg gcggacggct tccttgggag gaggttcgga gcccgcggct gcatcgccga
     5761 tgcccgtggt tggaatcagc ttggagacgc tgtcccattc cctatccagg accttgcgga
     5821 cgaacttgag ctggtgcgcc tcatccatcg cgcgcaggcg cttggctacc tgggtgcctt
     5881 tcgccttggc gacttcccga tccgcatagg gcccgatgtg aagcccctca cgcgagccga
     5941 aataccactg accgctgtag gcataaacct catcggctct gaaccactgc ggcagttttc
     6001 cgggcacgct gggctccaac gtggactcga tcacccgatt ctaacgtcgc gacctcgttc
     6061 gccgggcgag ctgcgtcgcg ttcagtgtgt gacgcagcgc aaacttccgg ccggctcgga
     6121 gaggggcaac aggccccgtc gccgtgcgca ggccctcacg acgacggccg cgcaaacctg
     6181 attcaccccg gctctcccat ttctgcgagc cggccgtgca ggcgggagat ccagccacgc
     6241 atcaggtcga tgccacgacc gtcgccaagt tccacctggt ccaccgtcga aagcgtcatc
     6301 agcacgacgc gcagggcgat gcgatagtcg cgcagcagct cgtccatcgt gtacccgtcc
     6361 acgccgcccg cctggagctc ttccaggtaa gcctcgagca agccctccac gaccgcgcgg
     6421 ccggccgcct cgtccagggc gctggagaga aagtaggcca cgtcgtacac cgccgggccc
     6481 cgccggacga gctgccagtc gatgacgatc acctcgtctt cacggaagaa cagattgtcc
     6541 agtcgcaggt cacagtgaag cagcgtggtc ggtgcctggt tcaagcggcg gatcaggtcc
     6601 aggccctggt cgggaaggcg tcgcagatag ggttccaggc gcgcctccag ctcggggccg
     6661 aagaaatccg agaatgtcga tcgcgagccc ttgaacatgc tgtgccgcat gcgcgcatcg
     6721 atgtcgtggg ggagcagcca gaacgcgtcg acgagctccg ctccccagca cgcggcgtgg
     6781 gcgcgggcaa tggcgcgcag agcccgttcg cagcgtcgca cgctgacgcc cgccacctga
     6841 tcgcccggct cggcatcgct gagatcttcg agcagcagaa tgtagcgccg cttcttggcc
     6901 gcggcgatcc gcagccccag cgcgggaagg aacgagagga gaaaccgcgg catgcggtcg
     6961 aagaggcgga gaatgtcttc ctgcttctcg gatgccctgt cgcggtcgaa atcgccgtaa
     7021 tacatgcgag gggtgctcag cggcaggctc cccgccattt ccatgtaaaa gcagttttcg
     7081 cgctcgtacg caccgagcat ctcccccatg gcgcggttat ccagcttcgg cagcttggcg
     7141 atcagcgagt caggaaggtt cccggggcga tcgtagctga gggacaacct cagcaggtca
     7201 cccaggaagc caacgccctc gccaagcacc tcggcctcaa tatcaacgac cttctgatcg
     7261 tccagaatcc cgccccggcg aagggcctcc gtcagccact cgggcgtcag cgcggctggg
     7321 gtttccggaa tcggcagcgg gccctctccg cgcccgctca tatcagcccc ctgatctccg
     7381 caaggctgtt gatggtgtaa tccggctcca ccgtctgcct gcccacctga agcggaggct
     7441 cgagaccctc ctcacgaatg agaaccgtgt tcatgcccac ctcgtaggcg ccttggacgt
     7501 cgtgctcggg ggaatcgccg acgaacagaa cctcgccagg cgcgaggccg ctcttctcca
     7561 ggctgatctc gaagaatcgc cggtggggct tgcacgactc cgcagcctct gagctcgtcc
     7621 agtgatgcag atagtcgtga agcccttccc gctcgacgag cgggtcgagc atgtcgtcgt
     7681 cgatgttgga gacgatggag aggtacagcc cttccgactt cagatgcgaa agggtttcgg
     7741 tgcagtccgg cttgagctcg agcccgctca gcaccagctc gtgctgccgg gcgcggaacc
     7801 aggcgtaggc ggcctcgtcg aactgcccct ccatctgctc cacaaagcgc acgaaggtgt
     7861 cgcggaagag atcccggtgc aggtagaagt cccgctcggc gtaagcgccg ttgacctccc
     7921 gggatgcaag cccgtacgcc ttgccgatgc tgcgcggatc gccagtaatg cccatgcgcg
     7981 cagccgcctc gagcagcgcg gggccggtgg agcggcccat gttgcgatag ctgaacagcg
     8041 tgccgcccag gtcgaagaac acgcccctga tgcgcgtcgt catggcgaaa cgatgctcac
     8101 gcgatgcgcg gccatcggcc aaaacgccgg aatttccaac cgatcacccg gctttctccc
     8161 gcagatcggg gagcacgtaa ccctgagcct ccaggtccgc gcggacgatt ttcttgtcca
     8221 tcttgccggt ggacgtcagc ggaatgctgt caacgtaaag cacgtcatcg gggagctgcc
     8281 actttgcaaa cgcctggctg cagtgagcca gcacggtctc caggctgacg tcggctccgt
     8341 cgccgagcac cacgagcgct accggacgct catcccattt cgggtgcggc tgcgccacca
     8401 cgcaggcctg ctcgaccccg tcgagagaga cgatgtggtt ctccaggtcc acggaggaga
     8461 tccactcccc gccgctcttg acgagatcct tggagcgatc gctgatgatc aggtactcct
     8521 cgctgtcgat cttgccgacg tcgcccgtga tcagccagcc gtcatgaaac ttctccggct
     8581 ggggatcgtg gtagtactcc gagcagatcc agggtccacg gatgaggatc tcgccgacgg
     8641 tttcgccatc gtggggcagg cggttgaaat cctcgtcgaa gatgtcgagc tccagaccgg
     8701 gcatcagctg tccggctttg gcgacgttgc ggaactgatc atcctcgctg agctcgaggt
     8761 gggaacgctt catcacccgc cgcgacagcg tggccagcgg cgacgtctcg gtcatgcccc
     8821 atccctgaat catctctacc ccgagggtgt cccagtacca gcggatcagc gacggcggcg
     8881 gcgcggagcc gccgcaggcc atgcgagcca ggcgggaaag gtcgtagcgc tccgggtcgg
     8941 cctcgaccag cgctttgatg ccctgccaga tggtcggcac gccggctgac atggtcacgc
     9001 cctcggacac cagcagatcc agcagcctcg acgtatccat gaaccggtgc ggcatgacct
     9061 gcttgcagcc cagcatgagg cacgaccagg gcatgcccca gcccatggcg tgaaacatgg
     9121 gcacgatgcc gagcacgcaa tccgtggcgg agaggcccat ggagtcggtc atgcactgag
     9181 tgagcgtgtg cagatactga gaacggtgct cgtactcgac gcccttcggg ttgcccgtgg
     9241 tgccgctggt gtagcacagg ccgagggcgg agttctcgtc gatccggggc cagtcgaaac
     9301 gggtcggctt gccggcaatg aattcctcgt agtcgacggc ctcggccacc tcggtgctcc
     9361 agccttcgaa accatcctcg gtggcgacca cgatccgctc cacgctggga atccgtccct
     9421 tgagcggctc cagcagcggc aacacgtccg catccgcaat gatcacgcga tcctcggcgt
     9481 ggttgatgat gtactcgagg tccttggccg aaaggcggat gttcagcgta tgcagcaccg
     9541 cgcccatgcc gccgatggcg tgatacgcct ccagatgacg ggacccgttc cacatgaaag
     9601 tcgccacgcg atctcccacg ccgatgccgg catcacgcag ggcgtgggcg agctggtggg
     9661 cacgatcgcg gatctcggca taggtctgcc gccgcgtgcc gttttcggta gcggtgacga
     9721 tctccacgtc gggcgccacc ttcgcgccac gatccagaat attggacatc agcaatggcg
     9781 tatgctgcat cgccacggtt cgccacctcc cttagacttt cgttccggtg aattcagcgt
     9841 gagggcagcg agtctatcat cctcagtgaa tgttgcgcgc ctgaccctgc cagtaggtct
     9901 tgcgaagctc ggccttcagg atctttcccg ccccgctctt cggcagctcg gcgtcgacga
     9961 actccaccgt cttcgggcac tcgtagccgg caaggtaggc acggcagtgc tcgatgacgg
    10021 ccgcctcgtc gagccgggcg ccaccgcgag gcacgatgat ggcgtgaacg gcctcgcccc
    10081 aggcatcgct cgggaccccg atcaccgcgc actcgtgcac tccggggtgc aggtacagca
    10141 cgttctccac ggccgcagag tagacgttct cgccgcccgt gatgatcatg tccttcaccc
    10201 gatcgacgag aaacagaaaa ccatcgtcgt cgaggtaacc ggcgtctccc gtgtgcatcc
    10261 agccaccgcg cagcgcctga gcggtgagct ccggctggcc ccagtatccc tgcatgacca
    10321 tgggtccgcg aacacagatc tctcccacct ctcccgacgc aagcggaagg tcgtcggggc
    10381 tcatgatggc cacgtccgcg ttgaaaacgg cacggccggc cgaacggatc ttccccgcca
    10441 gcggaccctc gaaggtatgg aactgcggct cgagcatcgt agccacagga gacagctcgg
    10501 tcatgccgta ggactgcagg aagcgggctc ccggcagccg ctccatggct tcacgcagaa
    10561 ggctctcggg catgggcgag gcgccgtagc agagaagatc cagggacgaa agatcgaagg
    10621 cttcgaagtt cggatgctgc agcagcgtgt tgagcatggt cgggacgatg acggtgacgg
    10681 tcaccccgaa gcgctcgatc gcccccagga actcggcggc gtcgaagcgg gggatcaccg
    10741 catgggtccc tccggccacg gtcagggtga acagccggga gccgccggcc agatggaaaa
    10801 gcggcgcaac atgaaggtgg acgctgtgct ccgagatgcc cagattcacc agagccgtca
    10861 tcgagttggc gatgaagttg tcgtggctga gcatcacgcc tttggcacgc ccggtggtcc
    10921 cgcccgtgta gaacagcgct gccagatccg ctccgccccg ctcggcgtcc tcgaccggga
    10981 gcgacgccga aaccagcgac tcgtagccca gcgccgcggt tcccgagccc tccacctctc
    11041 cgcaggagac caaatgagag acgctcccgc aggtttcgag cagctcgagc gcttccggga
    11101 ggtgggccgc gtccgcaagc agaaccttcg cgccgctgtc gttgaggcag tgcgccttct
    11161 ctggcgccgc ccagcggcag ttgaccggag caagaacgcc cccggcccat agcacggcga
    11221 agtagtactc cacatagcgc ggcgagttga gcgcaagcat ggcgactcga tcgcccggac
    11281 ccacacccag ctctctcagc gcgcctgcca ggcgcgccac gcgatcggca aactgctccc
    11341 aggtgatctc gccctccgcc gaaaccaccg cccgccggcg agggtagagg cttagggccc
    11401 ggtgaatgcc ctgggtgagg cgcatcattg aggaagtcat ggcggcgcgg gattctacag
    11461 gcagctcagg cgacgagcga gccagccccg gcggatcagc cgccgatcac cccggcggcc
    11521 ctgagcgcgt ccgcgtccag acccatttcc tgcagcagct cgtcggtatg ctcgccgagc
    11581 atcggcgcgt gccggtggat gccggccggc gtcctgtcga accgcgccgc cggtctcggc
    11641 tgacgaatgc ggccggccac cgggtggtcc agctcttcga ccatctcgtt gatcctcacc
    11701 tgctcgtgct cgaagacctc gtggcgatcc agcacggggg cgcagggaac gccttcggca
    11761 tcgagtcggg ccagccactc cgcgctgctg cgcgtctcca gcacctcggc agtcaggcgc
    11821 agccgctcct gcacgttgac gatccggccg ttgggcgtgt tgaaccgctc gtcatcaatc
    11881 cactcctcac gctctagcgc gcggcacata ccctgccact ccgcatcgga caccgcgcct
    11941 gccgtgatga agccgtcggt ggtggcaaag atgaggtcct gagcgaactg ggcccgcgca
    12001 gctttcactt ccttgccgat gaacgtgaag cccgccatgc cctccggcca gagataggcc
    12061 accatggtgt cgagcatggc tagcttcaca tgctgacctt cgccgctgcg ctcccgggcg
    12121 aagagggccg cggtgatggc ctgggccgcg gtaagcgccg tcgtcttgtc cggaatgatg
    12181 gtgcgcacca ttttcggcct tccggtttcc cggtcgcgtt ggatcgcggc aagaccggaa
    12241 agcgcctgaa tcaccgggtc gtacacccgc ttgtgcgcgt aagggcccgt ctctccgaat
    12301 ccgctgatag acacgtagac gatgtccggg cggatctccc gcaccacgtc ctctcccaga
    12361 cccatgcgct cgatggcccc aggccggaag ttctgaacga agacgtcgga agtggcagcc
    12421 aggcgcttga ccacgtccat gccggcctcc tgcttgaggt ccaccgcgat gctccgcttg
    12481 ttgcggttcg agctgatgaa cccggacgtg aggccatccc ggttgggccc catgtagcgg
    12541 acgagatcgc ccgtcaacgg ctcgaccttg atgacgtcgg caccctgatc accgagcagc
    12601 atggtggcca ccgggccgga aaccatgctg gtcaggtcga ggacccggac tccagttagc
    12661 ggtccgctca tatcaacctc tcaagtcagt tccgcgagcg ccgtgctttt cgcccacttg
    12721 tagtccgcct tgccgttggg cgcgcgctgc acttgcggca cccgcagaac ctgcttggga
    12781 agcttgtagc ctgcaaggtg gtcgcgggca aactcgatca attcgggctc ggatacgtcc
    12841 tggtcgtcgt gaaaggacgc gaccgccacc acgcgctcgc caaaacgctc atcgggaatg
    12901 ccgacgacca ggcaatcgac gacggccgga tggcgcttga cggcctcttc gacctcctcg
    12961 gggaacacct tctcgccggc cgtgttgatg cagacgctgc cgcgcccgag cagcgtgatg
    13021 ctgccgtccg cttccacggt ggcgtagtcg ccgggaaagc tgtaccgaac gccgtccacc
    13081 tcgcggaacg tttccgcaga tttgaccgga tccttgtagt agccgagggg cacgaagccg
    13141 ctggtggcga gcttaccgat ctcgcccgaa cccggctcaa cctcgcggcc ctcgtcagtg
    13201 aagactttca ccccttcggc gagctgaaac ttggccgtgg tcggccccga cgcccgggag
    13261 ctgacggagc tgcccatgct tccttccgtc gagcccatgg cgtccacgag catcatgtcc
    13321 cgatgacgca gcagcccttc cttgacctct ctgctccaca tcaccccgct ggagacgatc
    13381 tgccgcaggg aactgaggtc gtaaggctcg ccgcgacgct cggcctcgtc gagcgcggcc
    13441 agcagcggac gcgcaaaggc gtccccgaca acaacgagat ccgtcgcccg gtggctgccc
    13501 accagggtcc atatcagatc gggatccagc cccagctgcg gcgtcgtgat caccgtgccg
    13561 ccggtgagca gcggcgcgag gctgcccagc cacatgcccg tgccgtgcat cagcgggcag
    13621 gcgaccaccg taacgggcgg atcctctata cccgccacgt attcccgcag ggcctcgagg
    13681 ttctgcgggg gcaccaggcc gcgccccccg gcgcccatgg cggtgagcag gtagcagaac
    13741 tcgccgccgg ggtacatgac gcccttgggc atgcccgtgg tgccccccgt gtagagcatg
    13801 tacacgctcg ccggatcccg cttctgcctc gccatgggct cggcggcgtc gagcgagctt
    13861 tcgtagtcaa ccgaccccgc cagcagatcc tcggtcccgt catccacctg cacatagagc
    13921 ttcacgcggg gaagctgatc gcggatctct tcgatgcgcg gggcgtaaca cccctggtaa
    13981 aagaccgcct ctgcgtccgc gttgtccagc aggtaaacca gctcctccgc cttgtagcgg
    14041 tagttcacgt tgatcggaca gccgctgagc ttgaagacgc cgtactgagc ttcgagatac
    14101 tcgttgcagt tgtggagata caggcccacc ttcgtatccg cgcccaggcc gtgggcctga
    14161 agcagaccgg ccacccgcgt agcacggccg tcgtaatccg cccaggtgcg ggtcacgccc
    14221 tcgcagatga gggccgtccg gtccggaacc gtgtcggcga tggcctccca ggccgatgcg
    14281 aaatgaaaat ccatggcaga cgccctccct gtagaagaaa tccggattgg caatgggtcg
    14341 tccgtcctga catcgccagg ccgaccgaat tcgccaactg ttcgcagaaa tcgccaacac
    14401 cacaggcccg cccgtcgccc cttccacccg gcgaggcggc attaagcctg gagatcgggg
    14461 ttgttcggag ttggcatggc atttgaatat tcagtaacga ggccaacgga aacgacccgg
    14521 cttcagctgc aaatgaacgc gagcagacaa tgaaacggca aaaccagatg ataatcaaag
    14581 acaatgcacc cgcaggcccg atcgcgcgcc tgtctaggat cacctatccc cgggcttcgg
    14641 cggcaaacct gatgctgctg agcgtggtga tcgccgccat cggcgcgatt gcctacatcc
    14701 tgcaccccga tgccgcccag ccggcgccga acaccttcca cggcgcggcc ctgctggtca
    14761 gcaactggct gctgtgcggc tgacgacaga aagactccgg agagatccca gtgacaaatt
    14821 cgaacttgaa caacgtccgc agacgactgc gtctcgaccc gcgcaacggc tggattgccg
    14881 gcgcctgcgc cggcttcgcc aatcggttac agatcgaccc tgccatcgtg cgggtcggtg
    14941 tggtgatcgc cgccatgttc ttccccaaga cggtgatcgc ctgttacctg ctggcatggc
    15001 tggtgctgga cgaagctcgc tgagcccacc ggcggtgagc ccttccatca gcaggtgatc
    15061 tggcagggct cctccggcgg cagccagttt ggcgcgtcgt cggccagctc gcgaaagtct
    15121 atagccagct ccacgaaacg cgtgtacatg cgcaccgact gccgctcggc catgtggttg
    15181 agaagccagg cggcggtctc gcgccccagg ttctcgttgt tgtcgaagat ggcggtcagc
    15241 tcggccccga aataggcctc gcgattagcg gccagctccg cgaagcgatc cgcaaaggcc
    15301 ggttcggagc gggcctggag cagcttcatg aggtcacgct gccagcgccg ccgatactcc
    15361 gcccagagaa cccgctcggg aatgtagcga accgactgag ccaccagata ctcctgctgc
    15421 gccggagtca ggcgaccggc aaagcgggtg aagatgtcct ccacctcttc gcgccagcgg
    15481 gcctgggacg cttcgacgct cttgccgagc tcggtcttct cgatctcccg gttgctctcc
    15541 tccagccgtg ccgggaggcc ggccacctgc tcatcggtca tggtctgcat gatggcgacc
    15601 gcgaggggcg cgccggtctc gacgatctct ttcgcccagc ccatcatggt gttttccagc
    15661 gcgagcagtt cctgagcatc gacgccgtcc gaaagcgtcg tcggcacggc ctcgaagaag
    15721 tccgcataga gcggcatatg ctccgtgcga tgccagtaat gaagccgggc gaattcctcg
    15781 ttgaacaggt tccgctgagc gtcgttcatg ttcacgtaat cgttcacgcc ccaacgcacc
    15841 agccggtcga ggttgttgta ggcaaactga acgctgcagc cggaaccaag caccagcacc
    15901 gcgagcagcg caaacagacg gacgaccatg cgctcaatca tagatgaagt tcagaatcca
    15961 gcgcgccagc agcgcgcggt cgatctcgtt gtcgagcgag gccaaaccgg ccgcaagggt
    16021 ctcgggcccc agaagcgctt cgcggcggcg catcaacgtt tccgcatagt ccttctcgaa
    16081 ctccgggtgg ccctgccacg tcatggccac atcgccgatg gcaaacgccg cgttcgggca
    16141 gaaggcgctg gaggccacgc ggcgcgcgcc gcgcggcagg gccaccacct gatccttgtg
    16201 actcgacagc atgctcaccg atccctggtg cgggtccatc caacgttccc tgctcacgat
    16261 ctccacccca tggacgccta caccccaacc caccgaggcc ggctcggtgc ggccgccgaa
    16321 gtaatgggca atgagctggt ggccaaagca gatccccacg atcttttgtc cgccggccat
    16381 ggtgttaccg acgaactccg ccagctcgac gatccaggga tgatcctcgt aaacgctgtg
    16441 tcggctgccc gtgatgacgt aggcgtcgca ggccgccggc tcaggaagct cgccttcccg
    16501 cacgcggtag gtcgagaaac tcgggcgccg atgcgcatct ccgctgagca gatccacgaa
    16561 catggccggg tagtcgccaa accggggctg aatatcctcc agaacggcgc cggtctgcag
    16621 aatgccgatg cgcatcaggc aatgtttccc cgtcagatga tctcgaagta gcgttccagc
    16681 tgccagctgt tgaggttctt gcggtactct tcgcactccc agcgccggga catgacgtag
    16741 tggtcgacga aggcctcgcc gagcatcgcc ctggcatggt cggaagcctc cagacgatcg
    16801 gccgcggccg ccaggtcgcg gggaaaccta agctcctcgg gcagagagtc ctccacgtcg
    16861 taggaatttc cgaccacggg atctccgggc aggagggcct gctcgatacc ccccagcgcc
    16921 gcggcgcaca cggcggcagc gacgagatag gggttagagt cggcgcccgg gacacggcac
    16981 tcgatgtgct ggtgcgattc agatccattg atgacgcgaa tggccacggt gcggttatcg
    17041 atgccccagg tcgaagcggt gggcgcccag gctcctttca cgagtcgggt gtagctgttt
    17101 atggtgggag cgatcaacgc gaggtactcc ggcagatacc gctctaagcc ggccaccgcc
    17161 tgacgctgca gatctgacat gcccgacggc tggccgtcgt cgaagaaaac gtttcgcccg
    17221 tcggcatcga gcaagctcaa atgaaagtga ccgctctggc ccggatagtc catggaccac
    17281 ttcgccatga agctcgccat gcagtccagc cggttgaagt agactttcgc aaaggtcttg
    17341 aagatattgg cacgatcggc cgcttcctcg ccgagcgcgg tctgcaatgc cgcttcccac
    17401 accccgggac ccgtttcgca gtgaaggcct tccagatcgc agtccagcgc ctgggaaaag
    17461 tccatgagac cggagaactt gtcggcttcg ccgctggccc tcaacaggga gtaaccgaag
    17521 ttgcccgggg tcagcgggcg caggttccga tatcccttgt cccgcaccga atgcggcgtc
    17581 tcggcaaaga cgaaaaactc gtactcgaag ccagacttga gggtgagccc acggtcaccg
    17641 agcctgccca gcaccgtctt gagaagcgtt cgcgggcaca ggggatgggg accaccgtca
    17701 ccggccacga actcgcccag gaaaaacggg cacccctcct ccgaaagcca gcgctcgctg
    17761 tcggcaacca gccgatactg ggcatcagga aaacccgtat gccaccccgt ataggtaccg
    17821 gcgtcgtaga gctggtcgtg cacgtcccat ccgaagacgc agtcacagaa gcccccaccc
    17881 ttctccatca gcgaggcgaa cttggacaaa ctgacgtact tcccgcggat cacgccatcg
    17941 acgtcggtca gccccagctt gacacggctg acaccccgtt cacgataccg cgcgagcttc
    18001 tgatccagca gttccatacc gtcgttcaag taagatctcc tcgtttggcc tcaacatgtg
    18061 gagcagactt tacctcaggc gaagatttcc acgaattctg tggataactc tgaggataag
    18121 tcgggcacaa gttggcagag ccggcgttcc acggcaacca aagaatattg gtcacttttt
    18181 gttcaaattc aaaaaagatc atcaggatca gatggttaga tccccaaact aaccgaccat
    18241 atgaaaaatg ctcgccatct ccccatccca agttgccatt cttcgccagt gtgcataaca
    18301 cggccgtcgc gcttgttagt gttcgctaac aagccgtgct ccgcggcact taatccggca
    18361 gcggaaacgc ccgccccaga tgccgctcag tacaaccgtt agaccgcagt ggccgccgcc
    18421 ggcacgatcc ccgggtcagc cctcttcctg ctggtcctcg ggcagcttga tcgccacttc
    18481 tggggtgtca atcggcgaga tgtcggtgaa gatcgcggtg tccaggtgcc agtccacgcg
    18541 accctcctcg gcggccacct ggtgtgctgc ttcggcaccg gtgctcacca gcggtggcgc
    18601 ggcagcagcg gcatccgccg cgtggccgtg accgacgaag ctcaaggtca ccagacacgc
    18661 catcagggca atgaggaata cacgcatatc caagtcttct ttagggtcaa tgttcatatt
    18721 gcagttgcca gaaaattcga aatcctttgt cgtccatatc accggagccc tttgcggcca
    18781 ccggcaacgc caggctgacg cggctcgtca gccgaggcag cagatccgca tcccaaccca
    18841 cgcccaggct ggtcaggtga ccccaggcgg gcgtgagatc gttcaacccc tcgccgtacg
    18901 acgtatcggc aaagagcgcc atttcgccca gagggaccgg cgtgcgcagc tccagggaaa
    18961 gcagggcacc gcgatcggcg aggaacgcgt ccctctcgaa gccccgcacg cggccaaagc
    19021 caccgagcga caagcgcctg gagctcggca gctgggatcc gctgagctgt ccgagcagcc
    19081 tggccgccag cttctgctcc ccgggaagac cgggaacgtt gagcgcccgc cacgcatacg
    19141 cgttcaatcc cagccgccag aaggtcgcgt cctgaccggc gaaggtcccg tccccgaacc
    19201 gaccggcgtc cgcgacggcg ccgaagccgg cagcaacgcg tcggtcgtcc cacaaccgat
    19261 ggccgtcaaa gccggcgctg aggaaggtgg ccgcctgatc ctgcgcggtg gcccagctga
    19321 agcggtggtg gctgactccc agccgtgccg tgaggctcga cgcacggctg cggcgcaggg
    19381 ccagctccgt gccgacgtcg aagagcgccc cgctgccgtc cagctcgact ccgtcgcttg
    19441 cggtaaagct gttcgtggag gcgcgcacgt gggcctgacg gacaccgccg atgggcgtcc
    19501 ggtagtcgat gaacccgtaa gtctggttcg tcgggttgac ggtctgcagc agacccactt
    19561 ccagaacgtc gccgacgccc cgcgggttga accagccgcc caccacgctc agcctctcct
    19621 tgccggtggc gtcgtcgccg tgattgtcgg caatcacgcg agccgaccat ttgcgttgct
    19681 ccaccacgtt gacgttcaaa cgggtctcac cgacttcgct gccgggctcg aacgcggcct
    19741 gggccttaag ccccggaacc tgattgagca ggtagacgcg ccgcgtgatg gattcttccg
    19801 tgaccggctc ccccacgact tcctcgaatc gtgaggcgag ctccttctgg tcaaccccgg
    19861 tcaccaccac gtcgccaaga acgccaggca gcacgccgac ctccacgatc ccgtgctcca
    19921 cctgctgcgc aggcaggtag gcaacggcga gaaagtaccc ggcgtcccgg tagtaggcct
    19981 gcacccggcc ggcaatctcc tcgagctcca gcaccgagat gccacggcgg gccttctgct
    20041 gctcgatgag ggccatcaga tcttcggcgt ccgtggccga aagagccccg ccatcgcggt
    20101 gggcgccgag cgcgctgaga aatcgcccca cctcgtccag ctcggttcgg gtgaaaccgt
    20161 ggtcaccctc cgcgttgcgc cgacgaatct cgtcgagcgc gtcgtcgatc agcgcgttca
    20221 gatcgctttc cagcacgccg ggatacggct gaaaggcacg aacccgcagg gaacggactg
    20281 ccagcctcgg gctgctcatg ggatcagagc tgcgctcgct cacggccccg atgaccgtgc
    20341 cggcccgggc ctcgagggca accaagccga gaaccatcat cgtcaacgcg accatcacca
    20401 gccaggtcgc cgaacgcccg cgcggttgat ccggatccac agccgccccg gagccgacgg
    20461 tcacggcgtc acggcgctgg gtgtcgtcgt ccgaactcgc atctgcccgg aagttggcga
    20521 tcacctcgcg ccgggtcgcc cgatactccg aacggctgaa ctcgccggcg gcgtatgcct
    20581 ttgcgacccg ccgcaggcgc acggtttccg ggctgaactt catcagcgtc acgagatttc
    20641 cctcaccagc cggaatccgg tgaccgcatc tgcgcggccc tggtgatccc gcacggtcgt
    20701 cggcacgcag tcgccgatgg catcgtcgaa agcaccgcct gcaacccgca gcttgccacc
    20761 atccagcacc cattcctgag cgttgccgag cgcattgatc agcccgaact cgccggcctg
    20821 cccgctgctc gccgggacca gggccagacc acgctcgacg ccgtccacct ccacgcggca
    20881 gttgcggctg gcgtccggcg ctccctgggc cgccaggcgc cactccgtca gggtcggcag
    20941 ccgataggtg aagccggtga tctccgacag ccagcgtgca tatccctggg caaccacgac
    21001 atcgacgccg gtagccggaa gctggtccgc atccgcgacc ccgcacgcgc cggaataggc
    21061 acagtaggcg ttcagctgac ctcggctgat ctcgtgcctg gtaatggcaa atcgggcctc
    21121 gccgttcgga acgaccacca ggcgcggccc gacaagatcg ccgacgaggc gatcggcgca
    21181 atagccgctg cgtcccgcct gcgctccagc tccaaccagg tagcgggcgg agcagggatc
    21241 gagccggacc tcggcgagct ggggcagctc gccgaatcgt gtgcgggccg agtcggcaag
    21301 ggctgccgct ctcgccaggt cgtgctcgcc gagctgggcg acgcaaccgg cgacttttgc
    21361 gccgatcatc tgccggcccg gggatcccag ccctgcgtct tcggccaccc aggcgtcgaa
    21421 aagcgcagcc accggcccga ggtccaggcg caggcagctg gtgtcgagca cgtcggcaat
    21481 ggcggcttcg aagcgctcgg cgttctcctc tacggcccga agctgctcgg cagccgcgta
    21541 accgaactcg gcatcctcca cagcgcgagc cagccgctcc acggcggcaa cgtcgaagcg
    21601 cagcggctcg agctcggcga gggccgcctt ggcggccggg aaatcgcctc gatcgatgct
    21661 gcgaagcagc tgcgcctcca ccgcgtcgcc gacttcgagc ctgatcggct cgatctcctt
    21721 gctctcagga aaggttgagg ccagccgcgc aagctccctt cgcacggagt cgaaccagcg
    21781 ctgctcaggc tgcggcgact cgagcagcgc gaagactctg gcgagcaggc ggtcgtgaag
    21841 gaccctgcct gcccgctcgg gctcgccgaa tcgcgttgcg ctgcggtaga gctcgaaggc
    21901 attgcccagg tcccgagtcg cgacgatccg ttgcgtgagc agcgcgatga tctgagcact
    21961 catccgggcg ccacttgcgc cggattcgtc caggacgttc agccgatcca gctcgctggc
    22021 tatccggtct tcccagcccg gctcgaaatc cggctcgctg agcatcgcct ggacccgagc
    22081 cgtctgcgca tccaccagcg tcgactgccg aacctcctcc ctgacctcgg tgagctccgc
    22141 atcgcccacc aggtagttga tacccagcga gagggcgaat gccgctgcgg cggcgagcgc
    22201 cgtgcgcgaa cgccacggcg ccggtttgag cagatagtgc tcgacctcgt cgatggtcgc
    22261 cggtcgctca tggcgattga agcgcaggca cttttccagc acacgccact gccgccgggt
    22321 gagccgtctg ggcctgtccg gagcgagacc ctcgcgggcg gcttcgtcgg ccggcgtgcg
    22381 cccgtaggga tggtgcccgg tcaggatgag gtatatcacc accccgagcg cgtagatgtc
    22441 gtcccggggc tccggattgt ccccctgcag catctcgcgg cttgcgtacg ccggcgtcag
    22501 cgcacccaga cgaaccggat cgatgaccgt ctcttcaccg tagatctgat tgagccgaac
    22561 ggcccgggcg atgccgaagt cgagaacctt tgcggcattg cgcggcgaga cgaacacgtt
    22621 tccgggtttg aagtccgcgt gtaccacgcc cgtgctgtga gcgtggccga gcccggcgca
    22681 gatgccgcgg atgacctccc aggccatggg atcgggaagc ccgttcgggt acgcgcgcag
    22741 aaggtcggcg agttcctgcc cctccagcag ctccatggtg atgaagggga cgtcgccgtc
    22801 cttgtcgtaa tcgtagatgg acacgatgtt ggcgtgagac accgcctggg acttggccgc
    22861 ttcccgctga agggcgatga acgcctccgg atgggcgcgg aagttgtcgt tgagcacctt
    22921 tatggccagg tagggctgcc ggtcgcgcgc ttccactttg cgcaggtcct ttgcccggaa
    22981 caccgtgccc atgcctccgc taccgagctt ctcctcgagc acgaatcgct gcttgagaat
    23041 ccggggggcg ttggtctcac cgactccctc gcccgcgaag ctgacggaaa ggctcggatc
    23101 cgcccgtatg acggtggcgt cctgatgggc gggtctgctc acgtgacgtc gggcctcgcg
    23161 acgatcagcg acacgttgtc ccgagcggcc gtctcgaggc agcggctgag caggccgttt
    23221 gcgatctcag ccggcgactc gccctgaagc ccatgagcca ggtccgccgt gttcagctcg
    23281 cggtacaggc catcactgca gagcaggtag gtatcctggg ggtcgatgtc aaagaccgcc
    23341 acgtccagga acagctcgga gtgtccgccc accgcccggg taacgatgtt cgtatctgcg
    23401 gcaacggctt ccgactcgga gacggcaccg ctgtccagca ggtcggagac cgggttgtga
    23461 tcccgggtga tctgctccag gcggcgaccc cgcagccggt agaggcggct gtcgcccgcc
    23521 catagcgcca cgcccacgcc tgccagcgcg agcatcgcaa ccacggtgct gcccaccgtg
    23581 gcgccgggac aggtttcccg cgcatgctcg cgaagctgct cgttgacctc gccgagggca
    23641 tcatcgaccg catccacgtc gtcggccagg ttgccgttga gcgtcagcct gccaagcgca
    23701 tcggctatgg cgccgctggc gacgtcgccc gccttgtgac cgcccatccc atccgccacg
    23761 gcccagagcc gcgccgacgg ccggctcacc accgcgtcgt cgttgctcgc ccgccgcttg
    23821 ccgcagtgac acgcttcggc gctttgccag ctgagttcca tgacccctaa acgttctcga
    23881 agtgaatggc cgtcgcggga ctgagaggct cgccgaggct gatccagccg gccccccaca
    23941 gacgcacctc ccggcgcctg acaaaggtga cccgctcgtc ctcggtctgc acgaacgtgc
    24001 cgttcgtgga gtgatcgatg aggacgtact cctgctt
//
LOCUS       DBNHKV010000332         4565 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1180344, whole genome shotgun sequence.
ACCESSION   DBNHKV010000332 DBNHKV010000000
VERSION     DBNHKV010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1180344"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..314
                     /locus_tag="AC16PP_10435"
     CDS             156..314
                     /locus_tag="AC16PP_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482086.1"
                     /translation="MTKGNQDSVEKTVRDIRRATRRKHNPEEKIRIVLEGLRSEQSIE
                     PGGFTHAS"
     gene            366..1646
                     /locus_tag="AC16PP_10440"
     CDS             366..1646
                     /locus_tag="AC16PP_10440"
                     /inference="COORDINATES: protein motif:HMM:NF046778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6482087.1"
                     /translation="MKLEEAKVAVIGGSLAGCSIAIVLRRAGCRVDVYERSPTDLQDR
                     GAGIVLPNQLQSELIDRGFLPPDYPGCPIRQRDWVVHDGSFEGRVVWRQPVSGVANHW
                     GRLWRALRAAIPDACYHNGLGLIDYANSAEGISFQTSDGERRRADLLVAADGYRSLVR
                     EQMTWGSESDYAGYVLWRGNFEDSRVADRAVIARLDERQSWLSACYDGGHSVLYMIPG
                     AAETPGQDERRINWGIYSRPPDGLVLHEPSSIPEGSVTEAMYAQFDALLGDSLPAQIA
                     ALIRLSPREQVSIQPIYDELAVHFSEARVMLVGDAAAVVRPHTASGATKALQDALVFE
                     RLSREHDDLDALLAAYGAERLENCNALVEVGRRIGRAQVEETPEWSAMSPADFARWNE
                     ATLAGQSLYLYEDVSNTGGEAESHAGEPDVPPGP"
     gene            complement(2007..2522)
                     /locus_tag="AC16PP_10445"
     CDS             complement(2007..2522)
                     /locus_tag="AC16PP_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482088.1"
                     /translation="MERLNQWLGLVANFGVVIGIVFLAYEMRLNTDSMKSSTAAQSMA
                     AWNEIVLAAATNPDLIAEQIAANREGIRLDAGGLQISYFAGSMLKSTEFNYLEHARGN
                     LEQEQWDAYVYAQRWFMTYNTFLLHAWRMQRYTFASSFREFMDDLIRDICSKQECPEG
                     GRPEDWTELST"
     gene            complement(2603..3094)
                     /locus_tag="AC16PP_10450"
     CDS             complement(2603..3094)
                     /locus_tag="AC16PP_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482089.1"
                     /translation="MDIQGIAALLGNFGEFFGAIAVVATLAYLAVQIRQNTNALRSAS
                     YEHWNQQAAEFAHFQDQHAKELAETEDLASFDELTGEQFRYVACLLTIAANQAQCAFL
                     QHRAGTVDDDVFEARIGAFLFFIEKIPHRKETWGLITRESEVPAFVEYIEERASWLRP
                     ATA"
     gene            3195..3668
                     /locus_tag="AC16PP_10455"
     CDS             3195..3668
                     /locus_tag="AC16PP_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482090.1"
                     /translation="MDHQALAAMLGSYGEFFGAIAVVATLGYLALQVRQNTRSSYVTR
                     SAAVTNRLGEIQEAIVCNSDLSALMAKCRDPELTDLTPTEEERVQGIAELYVKTFSDV
                     QTAHSNSEMTDAYFATFRAEFRRIVETYPALKPRMRLTLKRFDMKLFPIYAPALD"
     gene            3804..>4565
                     /locus_tag="AC16PP_10460"
     CDS             3804..>4565
                     /locus_tag="AC16PP_10460"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6482091.1"
                     /translation="MSAMYKNTCGYRWLTLVILIWLGSAQAAVLEEIVVVAQKRDQSL
                     QDVGIAVSAFTGDQLDQLGWDTAEQVTAMSPGVTTIQPNGPSAFFTNIRGVAQNDFSG
                     DHQESPVAVYVDEVYISAASGAGFQLFDFERVEVLRGPQGTLFGRNATGGLVHYITRK
                     PSQELDGYIKGTYGDYDQLKFEGAVGGGITDKISGRLSFTTNNHDGIYKNRIGSNLND
                     GDDWAIRGQVLIDFNDRTSWLLNLRRGEQDVNVAPF"
BASE COUNT          945 a         1282 c         1387 g          951 t
ORIGIN      
        1 gactgctcaa ggaagtgggc gtagccatca accgcgcggc agatgtccta tgtggcatct
       61 ctgaggactg ttccccgtca gcccatgttg gacaactggc cctgattgag taagagacac
      121 ttttggctca tcgtttaccc gataaggaaa cgatgatgac gaaaggaaac caagacagcg
      181 tcgaaaaaac ggtgcgcgat attcgccgag ccacacgacg caaacacaat cccgaagaga
      241 agatccgtat cgtgctggaa ggcctgcgca gcgagcaaag cattgagccg ggcggcttca
      301 cacacgctag ttgaattccc gtatcattgc gcgccagtac cgccggcggg caaagggcag
      361 cggttgtgaa gctggaagag gccaaagttg cggtgatcgg cggcagcctg gcgggatgtt
      421 cgattgccat tgtgctccga cgcgccggtt gccgggtgga cgtttatgag cgttcgccaa
      481 cggatcttca ggaccggggg gcagggatcg tgctcccgaa tcaactccag tcggaactca
      541 tcgaccgggg cttcctgccg ccggactacc cgggctgtcc gattcgacag cgtgattggg
      601 tggtgcacga cggctcgttc gaagggcggg tagtctggcg gcaacccgta tctggcgtcg
      661 ccaaccactg gggccggctg tggcgcgctt tgcgcgctgc cattcccgat gcctgctacc
      721 acaacggtct gggtttgatc gattacgcaa acagtgccga gggcatctcc tttcaaacgt
      781 cggacggcga gcgcaggcgt gcggaccttc tcgtggcggc agacggttac cgctcgctgg
      841 tccgcgagca gatgacctgg ggatctgaat cggactatgc gggttacgtg ttgtggcgcg
      901 gaaatttcga agactcccgc gttgccgatc gggccgtcat tgcgaggctg gatgagcggc
      961 agagttggtt gtctgcctgt tacgacggtg ggcacagcgt gctctacatg attccggggg
     1021 cagccgagac gccggggcag gacgagcggc ggatcaactg gggaatctat tcccgaccgc
     1081 ccgatggtct ggtgcttcac gagccctcgt cgatccccga gggttccgtg acggaggcga
     1141 tgtatgccca gttcgacgcg ctgctcggtg acagtttgcc ggcgcagatt gctgcgttga
     1201 ttcggctcag tccgagagag caggtttcca ttcaaccgat ctacgacgag ctggccgtgc
     1261 acttttctga ggctcgggta atgctggtcg gggatgccgc ggccgtggtc agaccgcata
     1321 ccgccagtgg tgcgaccaag gcattgcagg atgccctggt gttcgaaagg ctgtctcgag
     1381 agcacgacga tctcgatgcg ctgctcgcgg cgtacggagc cgagcggctg gaaaattgca
     1441 acgcgctggt ggaagtgggg cggcgcatcg gcagggccca ggtggaggag actcccgaat
     1501 ggtcagccat gtcgcctgcc gacttcgccc gttggaacga ggccaccctc gccggccagt
     1561 cgctgtatct gtatgaagat gtaagtaaca cagggggcga ggctgaaagc cacgctgggg
     1621 aacccgatgt tcctcccggg ccctgagttc ttcaggcagt aatcgagaat ccgacatgag
     1681 aagaactctg ctgtctttgg cgccgacctg gcggtgatcc tcgtattcca tcccggtgcc
     1741 cgtgcgcctc agcggtcaga ggcggtcgct gatggcgtct cgaccaatcg attcatctct
     1801 cgacatggcg cgcctgtcgc tgggatctat ctcgggatct ggatccctct ggacgcttct
     1861 gccgctcgaa tggagaaggt ttgtccagcc catgtttttt ggtccactgg ttaagttgag
     1921 gatctcctgt tctcagttga tgttgtcgag cgggcgtcgt cccaatctat tctgaattgg
     1981 gacggcgaag aagcctttcg cttgttctag gtagacagct ccgtccaatc ctccggcctg
     2041 ccaccctctg gacactcttg ctttgagcag atgtcgcgga tcaggtcgtc catgaattca
     2101 cggaagctcg atgcaaacgt atatcgctgc atcctccagg cgtggagcag gaaagtgttg
     2161 tacgtcataa accagcgctg cgcgtagacg taggcgtccc attgctcctg ctcgaggttt
     2221 cctcgggcgt gctccagata gttgaactcc gtagacttca gcatgcttcc cgcgaagtag
     2281 ctgatctgta accctccagc atccaacctg atcccttccc gattggcagc tatctgctcg
     2341 gcaatcagat ccgggttggt ggccgcagct aagacgattt cattccacgc ggccatgctc
     2401 tgcgccgccg tactggattt cattgagtcc gtattcagcc gcatctcata cgccagaaac
     2461 acgatgccga tgaccacgcc gaagttggcg accaagccaa gccactgatt gagccgttcc
     2521 atggggtttc tcctctccct gtggatgagg agagactacg actttggcgg aagcgggtta
     2581 agaggaaggc gtcagaggcg gctcacgccg tcgctggtcg aagccagctc gcccgttcct
     2641 caatgtactc cacaaaggca ggaacctcgg actctcgagt gatcagcccc caagtttctt
     2701 tgcgatgcgg gatcttctct atgaagaaca ggaacgcccc tatccgagcc tcgaagacgt
     2761 cgtcgtcgac ggttcctgcc cgatgctgaa ggaacgcaca ctgcgcctga ttggcagcaa
     2821 ttgtgagcag acacgcgacg tacctgaact gctcgccggt caattcatca aagctcgcca
     2881 aatcttcggt ttcggcaagc tcttttgcgt gctgatcctg aaagtgcgcg aactcagctg
     2941 cctgctggtt ccaatgctca taactggctg aacgaagcgc gttggtgttt tgcctgatct
     3001 ggacggccag ataagcaagc gtagcgacca ctgctattgc gccaaagaat tccccaaagt
     3061 tgcccagcaa cgcagcgata ccctgaatat ccatgactca cctcctcctg tagatgaagg
     3121 gatggtagcg cagaagactt gcggcgcaga cggactgagg tagcctcatt ccattcattg
     3181 gagaggagac cgtcatggat catcaggctt tggctgcaat gctggggagc tacggagagt
     3241 ttttcggggc aatagccgtc gtagcgacgc tggggtatct ggcccttcaa gtgcggcaaa
     3301 acacgcggtc gagctacgtg actcgatcag cagcggtgac caaccgactc ggagaaattc
     3361 aggaagcgat tgtctgcaac agcgatctgt cggccctcat ggcaaagtgt cgtgaccccg
     3421 aactaactga tctgacaccc acagaagaag agcgtgtgca gggtatagct gagttgtatg
     3481 tcaagacatt cagcgacgtg cagacagcac acagcaactc agagatgaca gatgcgtatt
     3541 tcgcgacctt ccgtgcggag ttccgacgta tcgtcgagac ctatccggca ttgaagccgc
     3601 gcatgcgctt aacgctgaag cgtttcgaca tgaagctctt tcccatatac gcgccagccc
     3661 tggactaaac tcccgttgtc cacaacggga ccctagcaat gcgcaacccc cgacccatca
     3721 aaaaatgccg gccctggccg gttagattcg gtatcatctg gagttattca gcaacgggga
     3781 aaacgtaccg gggagggagc gcaatgagcg cgatgtacaa aaacacgtgt ggctaccgct
     3841 ggctgacgct ggtgattctg atctggctcg ggagtgcgca agccgcggtc ctcgaagaaa
     3901 ttgtggtggt cgcgcaaaag cgtgatcaga gtctgcagga tgtgggcatt gcggtatcgg
     3961 catttaccgg cgatcagctc gatcaactgg gctgggatac ggcagagcag gtcacggcga
     4021 tgtcgccggg cgtgacgacc attcagccga acggtccgtc agcgtttttc accaacatcc
     4081 ggggcgttgc ccagaacgat ttttcaggtg atcaccagga gtcgccggtg gccgtctacg
     4141 tcgacgaggt ctacatcagc gcagcttccg gtgccggctt ccagctattt gatttcgagc
     4201 gtgtggaggt gttacgtggt ccgcagggaa ccctgttcgg gcgcaacgca accggcgggc
     4261 tggtgcacta catcactcgc aagccctcgc aggaactgga tggctacatc aaggggacct
     4321 acggggacta cgatcagctc aagttcgaag gtgccgtcgg cggtggcatc acggacaaaa
     4381 tttccgggcg cctgtccttt acgaccaaca accacgacgg catatacaaa aaccggatcg
     4441 gttccaatct gaacgacggc gacgactggg ccatccgcgg gcaggtgctg atcgacttca
     4501 acgatcggac gagctggttg ctgaatctgc gtcgcggaga gcaggacgtg aacgtagcac
     4561 cgttt
//
LOCUS       DBNHKV010000333         6325 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_46070, whole genome shotgun sequence.
ACCESSION   DBNHKV010000333 DBNHKV010000000
VERSION     DBNHKV010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6325)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_46070"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="AC16PP_10465"
     CDS             complement(<1..541)
                     /locus_tag="AC16PP_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6482092.1"
                     /translation="MSRIAQWAIERPLYPWLIMAACFFGGLYGIETVGRLEDPNFPIK
                     TAYIITSYPGASAVEVEQEVTDVIEEALLELPYIDHIISKSVPGRSEVQVDLLEHYGE
                     GDVPQIWDELRRRVVEAAERMPPGAGLPLVEDDFGDVYGLLYAIAAPDYSPADIRDMS
                     RQISTTLKRVPGVAKVQTSG"
     gene            complement(545..1606)
                     /locus_tag="AC16PP_10470"
     CDS             complement(545..1606)
                     /locus_tag="AC16PP_10470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6482093.1"
                     /translation="MRRFLILLVLVCAGCEPAAEIVVEEQAVRPARIFRVSSEGVTVE
                     NQFVARVEAAQTVDVSFEVSGPLAELPVREGQTIDKGTLVAALDPTDFQLAVREAEVQ
                     LKLARQDLQRKRKLLADRGISQSVVDDAQALHDLRSVRLAQAREALEDARIVAPFDAY
                     VARRYTDNYANVQAGDKIVRLNDLNEIHVVASVPESLAATGSADQLIGAHAVFPFRPD
                     QRYPLTFRENLGEADAVAQTYEVTFAMPPPPNLQILPGMTATLELEMMAAEGEGRTMN
                     IPTSALIADSDKRFIVWIYDPETQAVSKRTVEVGPAVGRGVAVVSGLANGELIVATGA
                     SQLQNGMKIRVLGDPLTEL"
     gene            complement(1603..2151)
                     /locus_tag="AC16PP_10475"
     CDS             complement(1603..2151)
                     /locus_tag="AC16PP_10475"
                     /inference="COORDINATES: protein motif:HMM:NF037679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6482094.1"
                     /translation="MSAAGLAKTKILSAAAAVVGRSGAAHLTIDAVAAEAKLSKGGVL
                     YHFPSKRAILEGMLDRLISRYSERISRFEAELGDEPDAAIRAVILADRGQDAEERSMA
                     LALLAAAAEDPELLAPARAVIGQWFEAVEQSSGNVQLGILLLLATEGLRFLDMLDLLP
                     AGGTRREQLGQTLLNVAEAGRL"
     gene            2278..3453
                     /locus_tag="AC16PP_10480"
     CDS             2278..3453
                     /locus_tag="AC16PP_10480"
                     /inference="COORDINATES: protein motif:HMM:NF015963.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HIR6482095.1"
                     /translation="MPKWNNWSGRLAAEPDQIAFVRSEDDAAALVAAAKRKGHAVRTR
                     GACHSHAPLLVTDGTLVDLSGLSGVISANMETRQAWVWAGTPIYALGRALHDSGLALG
                     NQGDIDRQAIAGATATGTHGTGAALASLSASVAGARMVLADGSILDCGPDAERELWEA
                     ARLNLGAIGIITRLQLTLREPYRLREHGWTEPFDSLLPQIPHLAAGNRHFEFFWFPHN
                     DGAIVKTINETDDDPEYPLAEEGSRCAWSYEVLPNSRVQPHTEMEYSVPADRGPECLR
                     AIRELILSRFPDVRWPVEYRLLAEDDVWLSTAYGRPTATVSVHQDVALDETPYFRACE
                     EIFRSFDGRPHWGKVHYLEPDVLASIHPRWQDWWQARDRADPDGLFLNDYLAAWRPR"
     gene            complement(3467..4246)
                     /locus_tag="AC16PP_10485"
     CDS             complement(3467..4246)
                     /locus_tag="AC16PP_10485"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6482096.1"
                     /translation="MPEEFPLPNFNTDLSGEVALVTGTTSGLGRRFALVLAKCGAKVA
                     LTGRRVERFADLEAEIKAFGGECFSTRLDMTDAQDAIDVVGRVEEALGTVTILVNNAG
                     IPDAQRAHKMSLDLINAVIDTNLRGPYILSCEVARRLIDAKRPGRMVNISSVGGFRYD
                     GNGAALYSITKSAIARMSECLAVEWSRFNINVNCIAPGAFRSEMMDGMMERMGDFSGS
                     FPRKRLCDPAQLDSTLLYLCAPSSECVTGTIIKVDDGQGSR"
     gene            4378..4848
                     /locus_tag="AC16PP_10490"
     CDS             4378..4848
                     /locus_tag="AC16PP_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR6482097.1"
                     /translation="MATTLTVNGKAFTSDADPGTPLIFVLRNDLGLMGAKLGCGAEQC
                     GSCTVLADGEPIHSCTASLESISGRCIETLESLSRDGQPDAIQQAFMDENAAQCGYCT
                     AGIILAAKALLSKRPDPTRAEIVEALNGHLCRCGAHPRIIRAIERAAAARGQTS"
     gene            4845..>6325
                     /locus_tag="AC16PP_10495"
     CDS             4845..>6325
                     /locus_tag="AC16PP_10495"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR6482098.1"
                     /translation="MTLPPSLARTPRLDRWLRLDPDGSVAICTGKVEIGQGIKTALAM
                     IAADELDVALYRVRVRTADTALTPNELITAGSLSVEDSGGAIRVACATARAKLLDAAA
                     AVLGVPRDSLDVEDGTVTSSLTNQRTDYWSLPYDDLFSVEIQEPLPLKNPDSYRVVGR
                     PAQRLDLPAKVLGHPAFVHDMSLPDMLHGRLVKPPLPGARLERFPDDFQAPGLVAVVR
                     DGSFVGVVAEREEQAVRAAERLSAQCTWHRAPLSPLPSQMAAHLRENVAASFPVIHGG
                     PVAEPVPPAPEIRGTAKRLSATYTRPFQMHGSIGPSAALAQLESGRLTVYSHSQGVEL
                     LKIALADVLGMAEDSVRVVHAEGAGCYGHNGADDVALDAALLARAVTPRPVLTKWTRA
                     DEHGFEPYAPATVIEMTASLDDRGQILHWDHQAFGLTHMGRPRPSPGHSNLQSAWWLS
                     SPIPPVPREPLMFPEAGIHRNLQPIYDFPDQRLVKHFVADGPLR"
BASE COUNT         1122 a         2142 c         2023 g         1038 t
ORIGIN      
        1 cgccgctggt ctgcaccttg gccacacccg ggacgcgctt gagcgtggtg ctgatctggc
       61 gggacatgtc acggatgtcg gccgggctgt agtcgggcgc tgcgatggcg tagagcaggc
      121 catagacgtc accgaaatcg tcttctacga gcggcagccc cgcacccggc ggcatgcgct
      181 cggccgcctc aacgactcgg cgcctgagct cgtcccagat ctgcggcacg tctccctcgc
      241 cgtaatgctc gagcaggtct acctgcactt ccgaccggcc ggggacggac ttcgagatta
      301 tgtggtcgat gtagggaagc tccagcaggg cttcctcgat gacgtcggtg acctcctgct
      361 ccacctccac tgccgaggcc ccggggtagg acgtgatgat gtaggcggtc ttgatcggga
      421 aattcggatc ttcaagcctc ccaacggtct ctatgccgta gaggccaccg aagaagcagg
      481 cggccatgat caaccagggg tagagcggtc gttcgatggc ccattgcgcg atgcggctca
      541 tgattcagag ctccgtcagc ggatcgccca gcacgcggat cttcatgccg ttctggagct
      601 ggcttgcgcc ggtggcaacg atcagctcgc cgttggccag cccggaaacc accgcaactc
      661 ccctgcccac ggccggaccc acctccaccg tccgtttcga cacggcctgg gtttcgggat
      721 cgtagatcca gacgatgaag cgcttgtcgg aatccgcgat cagcgcagag gtgggaatgt
      781 tcatcgtccg accttcgccc tctgctgcca tcatctccag ctcgagcgtg gccgtcatgc
      841 ccggcaggat ctgaagattc ggtgggggcg gcatggcaaa cgtcacctcg taggtctgcg
      901 caaccgcatc ggcttcgccg aggttttccc tgaaggtcag cggatagcgc tgatccggac
      961 ggaaggggaa aacggcgtga gcgccaatca gctgatcggc cgaaccggtc gcagccaggc
     1021 tttccggaac gctggcaacc acatgaatct cgttcaggtc attgagccgg acaattttgt
     1081 cccctgcctg gacgttggcg taattgtccg tataccggcg cgcgacgtac gcatcgaagg
     1141 gcgcgacgat gcgggcgtcc tccaacgcct cccgtgcctg ggcaagcctt acgctgcgca
     1201 gatcgtggag tgcctgagca tcgtcgacca cggactgaga gataccgcgg tccgccagca
     1261 gcttccgctt gcgctgcaga tcctggcgcg cgagcttgag ctggacttcg gcttcccgca
     1321 ccgcaagctg aaagtccgtg gggtccaggg cagcgaccag ggtgcctttg tcgatggtct
     1381 gtccttcccg aacggggagc tcggcgagcg gccccgacac ctcgaaggac acgtccaccg
     1441 tctgcgcggc ttccacccga gccacgaact gattctccac ggtgacgcct tcgctggaaa
     1501 cccgaaaaat ccgggccggc cgaacggcct gttcctcgac gacgatctcg gcagccggtt
     1561 cgcaaccagc gcagaccagc accagcagga tcaggaagcg cctcacagcc gtccggcctc
     1621 ggcgacgttg agcaacgtct gcccgagttg ctctcggcgg gtgccgcccg cgggcaggag
     1681 gtcgagcatg tccaggaatc gcagaccttc cgtggcaagc agcaggagaa tgcccagctg
     1741 gacgttgccg gacgattgct ccacggcctc gaaccattgc ccgatgacgg cccgcgccgg
     1801 cgccaggagc tccgggtcct cggcggcggc agccagcagc gccagcgcca tggagcgttc
     1861 ctcggcgtcc tgcccgcggt cggccaggat gaccgcccgg atggccgcat ccggctcgtc
     1921 gcccagctcg gcctcgaaac ggctgatccg ctccgaatag cgggatatga gacggtccag
     1981 catgccctcc agaatcgcgc gcttgctggg aaaatgatag agcacgccgc ctttggagag
     2041 cttcgcctcg gcggcgacgg catcgatggt cagatgggca gcaccgctgc ggcccaccac
     2101 cgccgcggcg gcgctgagta tcttcgtttt cgccagacct gcagcactca tggccgcaaa
     2161 cgtaccgcaa aaaaactata ccgtccagac ggtatagttt cgttgcgtag aatgcggcgc
     2221 taccgtatgc gcagatccag ccccacgacg ggcagaggac acgcaaagga cacgcatatg
     2281 ccgaagtgga acaactggtc cggccgtctc gcagccgagc ccgatcagat cgcgttcgtt
     2341 cgcagcgaag acgatgccgc cgccttggtt gccgcagcaa agcggaaggg tcacgcggtg
     2401 cgcacgcggg gcgcctgcca cagccacgcc ccattgctgg tcacggacgg gaccctggtg
     2461 gacctgtcgg gtttgagcgg cgtgatctcg gccaacatgg agacgcgcca ggcgtgggtg
     2521 tgggcgggca ccccgatcta cgccctcggg cgagcgctgc acgactcggg acttgccctg
     2581 ggcaatcagg gcgacatcga ccgtcaggcc atcgccggag ccaccgcaac gggcactcac
     2641 ggcacggggg ccgctctcgc cagcctgtct gccagcgtgg cgggagcacg catggttctc
     2701 gccgacggga gcatcctaga ttgcggaccc gatgccgagc gggagctgtg ggaggcggcc
     2761 cgcctgaatc tgggcgccat tggcatcatc acccggttac agctgacgct gcgcgagcct
     2821 taccggctgc gggagcacgg gtggacagag ccgttcgact cgctgctgcc gcagatcccg
     2881 caccttgcag caggaaaccg ccacttcgag tttttctggt ttccccataa cgatggggcg
     2941 atcgtcaaga ccatcaacga gacggacgac gaccccgagt acccgctggc agaggaaggg
     3001 tcccgctgcg cctggagcta cgaggtgctg ccgaactcac gcgtgcagcc gcacaccgaa
     3061 atggagtaca gcgtccccgc agaccggggc cccgagtgcc tgcgcgccat acgggagctc
     3121 attctcagca ggttcccgga cgtgcgctgg cccgtggagt accgcctcct cgccgaggac
     3181 gacgtgtggc tgtccacggc ttacggccgc ccgactgcca ccgtgtccgt tcaccaggac
     3241 gtcgcgttgg acgagacacc ctacttccgc gcctgcgagg aaatattccg cagcttcgac
     3301 ggtcgtcccc attggggcaa ggttcactac ctggaacccg acgtgctggc gagcatccac
     3361 ccgcgctggc aggactggtg gcaggcccgc gaccgagccg atccggacgg cctgttcctg
     3421 aacgactacc tcgccgcttg gcggccccga tagcgaagcg atccggtcag cgcgagccct
     3481 ggccgtcgtc caccttgatg atggtgccgg tgacgcactc cgatgacggc gcgcacagat
     3541 aaagcagggt gctgtccagc tgagccggat cgcacagccg cttgcgcggg aagctgccgg
     3601 agaagtcgcc catccgctcc atcatcccgt ccatcatctc cgacctgaaa gcgcccggcg
     3661 cgatgcagtt gacgttgatg ttgaatcgag accactccac ggccaggcac tcgctcatgc
     3721 gcgcgatggc cgatttggta atggagtaaa gggcagcccc gttaccgtcg tagcgaaacc
     3781 cgcccacgga gctgatgttg accatgcggc cgggacgctt ggcgtcgatg agtcgacgcg
     3841 ccacctcgca ggaaagtatg tacggacccc gcaggttcgt atcgatgacg gcgttgatca
     3901 gatccaggct catcttgtgc gcacgctggg catccggtat gccggcgttg ttcacgagta
     3961 tcgtcacggt gccgagcgct tcctccaccc gtccgacgac gtcgatggcg tcctgcgcat
     4021 cggtcatgtc caaccgcgtg gaaaaacact ccccgccgaa ggcctttatt tctgcttcca
     4081 ggtcggcaaa gcgctctacg cgcctgccgg tgagcgccac tttggcgccg catttggcga
     4141 gcaccagggc gaagcgacgg ccgagcccgg acgtcgtgcc cgtcaccaga gccacttccc
     4201 cgctgagatc cgtgttgaag ttcggcaaag gaaactcttc tggcatgttg gtctatcccc
     4261 agcgttgatt aaacgaggca agttagcact tgtgctcacc gttcactacc ggcgatcctc
     4321 gcgagcaacg atggcgacag ctggcataat tctgaaaacc cctgcaggtt gccgaacgtg
     4381 gccacgactc ttaccgtaaa cggcaaggcc ttcaccagcg acgcggatcc gggcacgcct
     4441 ctcatcttcg tgctgcgcaa cgatctcggc ctgatgggcg cgaagctcgg ctgcggcgcg
     4501 gagcagtgcg gcagctgcac ggtgctggcc gacggggagc cgatccatag ctgcacggcg
     4561 tcgctggaaa gtatcagcgg gcgttgtatc gagacgctcg agtccctgtc ccgggacggc
     4621 cagccggacg caatccagca ggcattcatg gacgagaacg ctgcccagtg cggctactgc
     4681 accgccggca tcatcctggc tgccaaggcg ctgctgagca aacggccgga cccgacccgg
     4741 gccgagatag tcgaggcgct gaacggccac ctgtgccgct gcggcgcgca cccgcgaatc
     4801 atccgcgcca tcgagcgcgc cgctgccgca cgtggacaga cgtcatgaca ctgcccccat
     4861 ccctggcgcg aacgccacgg ctggaccgat ggctgcgtct cgacccggac ggaagcgtgg
     4921 cgatctgcac agggaaggtg gagataggac agggaataaa aacggcgctg gccatgattg
     4981 cggcggatga actggacgtc gcgctgtacc gcgttcgcgt tcggacggca gacaccgctc
     5041 tcacgccgaa cgagctcatc acagcgggaa gcctctccgt cgaggacagc ggcggcgcca
     5101 tccgtgtcgc gtgcgcaacg gctcgggcga aacttctcga cgccgcggcc gcagttctcg
     5161 gcgtgccccg ggactctctg gacgtggagg acggaaccgt aacgtcctct ttgacaaacc
     5221 agcgaacgga ctactggtcc ctgccctacg acgatctgtt ctccgtggag attcaggaac
     5281 ccctgccgct caagaacccc gacagttacc gggtagtcgg ccgcccggca cagcggctgg
     5341 atctgccggc aaaggtgctc ggccatcccg ccttcgtcca cgacatgagc ctgcccgaca
     5401 tgctgcacgg ccggctggtg aagccgccct tgccgggcgc caggctggaa cgttttcccg
     5461 atgacttcca ggcacccggg ctcgtcgccg ttgtcaggga cggcagcttc gtcggggtgg
     5521 tggccgaacg ggaggagcag gccgtgcgcg ccgccgagcg tctctcggcg cagtgcacct
     5581 ggcacagggc gcccctttcg ccgctgccct cccagatggc cgcccatctc agggagaacg
     5641 tcgcggccag cttccccgtc atccacggcg ggccggtagc cgagccggtc ccgccggcac
     5701 ccgagatccg ggggaccgcc aagcgcttga gcgcaacgta cacgcgcccg ttccagatgc
     5761 acggatccat cggtccgtcc gcagccctgg cccagctcga gtccggacgc ctcaccgtgt
     5821 actcccacag ccaaggcgtc gagctgctga agatcgccct ggcggacgtg ctcggcatgg
     5881 ccgaggacag cgttcgggtc gtgcacgccg aaggagcggg ctgctacggg cacaacggtg
     5941 ccgacgacgt cgctctggac gccgccctgc tggcgcgagc cgtgacgcca cggccggtgc
     6001 tcaccaaatg gacgcgagca gacgagcatg gctttgaacc atacgcgccc gccaccgtca
     6061 tcgagatgac cgcgagcctc gacgatcgcg ggcagattct gcactgggac caccaggcgt
     6121 tcggcctcac ccacatgggc cgcccgcggc cgagcccggg acacagcaat ctgcagtcgg
     6181 cgtggtggct gtcatcgcca atcccgccgg tgccgaggga gccgctcatg tttcccgagg
     6241 cgggcattca ccggaatctg cagcccatct acgacttccc ggatcagcgc ctcgtcaagc
     6301 acttcgtggc cgacgggccc ctgcg
//
LOCUS       DBNHKV010000334        21409 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_543735, whole genome shotgun sequence.
ACCESSION   DBNHKV010000334 DBNHKV010000000
VERSION     DBNHKV010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 21409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21409
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_543735"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="AC16PP_10500"
     CDS             <1..1389
                     /locus_tag="AC16PP_10500"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HIR6482099.1"
                     /translation="LHDLYGKAFEARYEAYEEMTRSGGIKLFKRIRAQELWRKMLSML
                     FETGHPWITFKDACNVRSPQSHVGVVHSSNLCTEITLNTNGEEIAVCNLGSVNLVQHV
                     RDGELDTRKLKQTVRTAVRMLDNVIDINYYSVPQARNSNMRHRPIGLGIMGFQDALYK
                     LRIPYASEDAITFADRSMEAVSYYAIDASCKLAKERGAYASFSGSLWSRGILPIDSLK
                     QLHQERGAQYLDANFDATMNWDKLRGRVQINGMRNSNVMAIAPTATIANITGVSQSIE
                     PTYQNLYVKSNLSGEFTVVNPYMVHDLKDRGLWDKVMVNDLKYYDGSVLSIDRVPDEL
                     KQLYATAFEVEPRWLVDAASRRQKWIDQAQSLNLYIAGASGRKLDVTYRMAWLRGLKT
                     TYYLRSLGATSTEKSTLDRGRLNAVSATQADPTTAARAPTPLDMDSVTAGGPAQVPLA
                     CSIDDPDCEACQ"
     gene            1403..2761
                     /locus_tag="AC16PP_10505"
     CDS             1403..2761
                     /locus_tag="AC16PP_10505"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020800276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HIR6482100.1"
                     /translation="MLSWNDFEEEHANAETAMQQPPAEAPLESKEDAAASFPPPGEAV
                     AGAGAVAEAAAGAAAPAPRPVEELPPITVSEPAAPLSEAPSNDEVLQRAEAAIGEMGL
                     EGLAEDGIEENYQRVTVDQKRMINCRADLNQLVPFKYDWAWQKYLDGCANHWMPQEIN
                     MTADIALWKSESGLTQDERLVVKRSLGFFSTADSLVANNLVLALYRLITNPECRQYLL
                     RQAFEEAIHTHAYQYCIESLGMDEGEIFNMYHEVPAVARKAGWALKYTRDISDPTFQT
                     GTPQADKALLKNLIAYYCVLEGIFFYCGFTQILSMGRLNKMTGTSEQFQYILRDESMH
                     VNFGIDVINQIKLENPGLWDEELQTLARQMILEGTQLEIEYAKDTMPRGILGMNANMM
                     AEYLQFIANRRLAQIGLAEQFAGAKNPFPWMSEIMDLKKEKNFFETRVTEYQTGGALS
                     WD"
     gene            complement(2786..3133)
                     /locus_tag="AC16PP_10510"
     CDS             complement(2786..3133)
                     /locus_tag="AC16PP_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482101.1"
                     /translation="MAGMRGLVFGMLMFLSASSAAREMQVEGDVVRVIPLTASSVVVE
                     RSGDCAPVKPIGQADLVALLAWDLRADCHTQRRKVEVQDGYRVYYRWDERVYSVVMTE
                     RPGPTIPLRVNVR"
     gene            complement(3136..3831)
                     /locus_tag="AC16PP_10515"
     CDS             complement(3136..3831)
                     /locus_tag="AC16PP_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR6482102.1"
                     /translation="MSRAAPLLAALLLCGTGHAVTQYPYLSCFEAASRLHDVPVDLLL
                     AVAATESNWDPDARSAANAHGIMQIQWPGTARHLGVGRLSELYNPCLNIERGAKYLSE
                     LLARSGGAEDRALAAYNYGPSRIASSSTLPAGALRYVSTVQGHRERIVRKTGARQASA
                     TRAAEHAVRFDTAHRARRYARSLDERVEGADFVTRAEPEGGFAVVMRIAAGGLSLTDL
                     NVLERLGWPVRGD"
     gene            complement(3828..4442)
                     /locus_tag="AC16PP_10520"
     CDS             complement(3828..4442)
                     /locus_tag="AC16PP_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482103.1"
                     /translation="MKFLLGLILGGAMVVVAAQRLAVDGPDSGVSSLGAWLTGMRAAA
                     GQFEPGWAAREDQPTALPAQTDPAAGDAEVDAESLGAGPAEVAEGPLAADMQEPMVRL
                     AGPEPLPPAEGPEPLPPAVRLLPAADPPAGSASETIQQVWAPFRSQTSAEGFARRLSS
                     ETEHPFGIDRRGPGRYQVFFAYADEEERRRLLEQVRPLIGTGAP"
     gene            complement(4452..5813)
                     /locus_tag="AC16PP_10525"
     CDS             complement(4452..5813)
                     /locus_tag="AC16PP_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482104.1"
                     /translation="MSAARCLATWLLLATLPGLASAAPGGELLGGSLGRWLDETAGPE
                     LAETLARHPKFKGETVRFVPMLDGRAEPAGNRLVQAVEQRLTRHLIREGGVRIAWERD
                     RQPCAAPEEVPYLLGIEIQQRGPDIYHLNIAMVDVDEGVWVSGVSLAWKGRLLPTERT
                     ALGTRANGGPPGSMQSPLPLSDSSVIGELMLAGLGCAIPAGLDGSLYVAMPVESDLSG
                     VAQALTRQLSVRPLAALTIDRAQASWEMALTARPLGNGTDELVATLRDLRREGSAQQI
                     ASVVISRPRSERPGPVTPLGMPAALLSGLSYETARSRGVCQSSTGPGGCIEVSFDLLR
                     PAYLLVVSSRDERLESQVCGRSLRIAEPGPRRYRIRVPSGAATASRPDAGVYVLAVEK
                     RSVAHRIARQINAGPGACPGGVPESTGQWLQALGRVLAEYGGDVDWRAAHFRGGPGGI
                     TRI"
     gene            complement(5800..6510)
                     /locus_tag="AC16PP_10530"
     CDS             complement(5800..6510)
                     /locus_tag="AC16PP_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482105.1"
                     /translation="MTFEPVNHAADGDGLQTDVMRFMAIIAFCLIAVLALVKNAEPAV
                     DPRPAPSVQQPVGMAQASELAAPEVEPRAAVRPARPQPVPPVATGTPQERPAEVEDVE
                     DVEDVEKVPGEPAAPHGLALRFASDRDFLRLIETGRVRVFAFQPDDPGGTALGLGRNL
                     EFFASPPPGRVYELQADTIPSRVTASLARAAGNPGGFSWGVRLPVELEASIRSWIDQD
                     VIGELIIDRYAQVRHVGS"
     gene            complement(6507..7115)
                     /locus_tag="AC16PP_10535"
     CDS             complement(6507..7115)
                     /locus_tag="AC16PP_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482106.1"
                     /translation="MKGVAEFVCGAGAGAGLALVALGLSGGAVAADAAPVSDWLQLNL
                     GHSAWLFALVLTVYAANLHRLSRLLAGGPDLKVVVKLDQLSDVWINLFVGIGVIWTAV
                     GMRSALQAALGDPGGALSGSAGSVLHKLVDGGILLALTTTIVGGVGGYVMRLVKTMVV
                     GARLQTFYSGVSRADLRELVGLGERIEARLARLDPQEPAVVA"
     gene            complement(7116..7652)
                     /locus_tag="AC16PP_10540"
     CDS             complement(7116..7652)
                     /locus_tag="AC16PP_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482107.1"
                     /translation="MFRRIFVPAFFISLVAGCTSTETVDACAVRFQDDLERSMSLAED
                     RLENGCEYRFDGYFQSLLAIAAENPDSRNPMLFSDHLMRVNDMGVISKRQAELLYNRY
                     FNVKFVSLRGDYNTCSQTCPMRDQVMSDMRAELHDKELGLMRASADRASFYRADTLLK
                     EADLVLEATCRACGADGR"
     gene            complement(7677..8510)
                     /locus_tag="AC16PP_10545"
     CDS             complement(7677..8510)
                     /locus_tag="AC16PP_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482108.1"
                     /translation="MDTNPRKGGSPLGRVLLPFLLLAAATASAVPDSGPVVRLFPTTV
                     LEDIRETGQVAKEMENSLQEVINRLDLQHQLYVESLCEGSDGDQGCERMAKQLGATYL
                     EMLTIMGDRLPEMELAVNNTRASLEKRLREELGHRTTPTSLQNALLGTTPASSAAAEQ
                     PALRGRSGVRLSDRFAQYYALVATQREGRGRSLAVVAADIYLDMEEASRLIAATQEEI
                     GRAALMEQLNQSFGLITPEMTEVVDGVKGILFGEASTDSPIAAPPYAIGESEFVSPLA
                     M"
     gene            complement(8573..9205)
                     /locus_tag="AC16PP_10550"
     CDS             complement(8573..9205)
                     /locus_tag="AC16PP_10550"
                     /inference="COORDINATES: protein motif:HMM:NF017269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HIR6482109.1"
                     /translation="MRAPALGLGLSLCVAAAPGMAGHSDGFPQAAPAGSHTHYTYADV
                     VGVEPLLEYRTVTEPRRDCRVAGYAAASSGTRSHRYTGPGHPDAHDHYQDHHDHGSPV
                     AAVIGGVVGGLLGSQFGSGSGKKAMTVAGALAGASVASSVSRSRSRHAGYTPRRCTTV
                     MTSREVEEVAGYRVRYVYQGEEFTKVVASDPGDRVRVQVQVTPAVGEPDW"
     gene            9399..10652
                     /locus_tag="AC16PP_10555"
     CDS             9399..10652
                     /locus_tag="AC16PP_10555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017905501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6482110.1"
                     /translation="MTKSDHHQILIIGGGAAGITVAASLKRRQGRHDLDIGIVEPSEH
                     HYYQPALTLVGAGTYPLEKTRRAESRLIPPGVAWHRDSATGFDPESNSVTLASGARLS
                     YDYLVVCPGLELNWERIDGLRETLGSNGVCSNYSPDHVEYTWQCLQSLQEGATALFTQ
                     APLPFKCPGAPQKIAYLAADHLQRRGLLGSCHLHFLTHGPALFAVPLFAAELSKVAER
                     WGIDVHFQTNLIRVDGPARQATFEKVGGEGEGEEVTMTFDMLHATPYQSPPAAVKESP
                     LANEAGWVDVHQHSMQHTTYANVFGLGDACSTPNSKTAAAVRKQAPVVVRNLLHLLDG
                     GSVEEAYDGYGSCPLTTAYGKVLLAEFTYGGKVTPTFPLDPRKERRSMWWVKKTALPI
                     MYWDYMLRGHEAFPGHDTEYEAPPV"
     gene            complement(10669..11556)
                     /locus_tag="AC16PP_10560"
     CDS             complement(10669..11556)
                     /locus_tag="AC16PP_10560"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6482111.1"
                     /translation="MARWDRDSGASIPDWFFEAVETEYATRSVEVDECDVVYQLWGDE
                     DKPGILLIHGMNAHSHWWDFIAPQLLDGYRVAAMNLTGMGDSDYRYEYDGATYAQEIR
                     AVCDHAGFDERAALVAHSFGGYMAVKAAVLFPDRFGALVLVDSGIRHPEEPVPDRPAM
                     GGRSKVYPDRETALGRFRVQPPQSCDNAYILEYIARHSLMPVDGGWAWKFDEDLPSTL
                     KDVERTPEDYRHLDVPTGLIYGAESELFSARTEDYMRELIAQDFPAVALPEAQHHLFL
                     DQPLAFVAELRKMLATLLA"
     gene            complement(11560..12690)
                     /locus_tag="AC16PP_10565"
     CDS             complement(11560..12690)
                     /locus_tag="AC16PP_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6482112.1"
                     /translation="MDFSFSDEQTLLQDSIEKFIQNDYAFDARQRVVREEQGFSSDNW
                     RTFAELGWLGVPFPEADGGFGGTAIESMIMSEQFGKGLVVEPFLATVVLAGGALKIGG
                     SPSQKEKYLAGVMDGSLQGALAYAEPQGRFNLADLTTTASPEGDGYVINGYKAVVMNG
                     PSADFLVVSCRTGGGQRDADGVSLFVLPADTEGVARRDYPTVDAFRASEVTLENVRVG
                     SDALIGEEGKGLAILEQVVDEAILAVGAEAVGCMEVLYKTTVEYCKTREQFGQPIGKF
                     QVLQHRMVDMFMEHEQAKSLMYMAAMRLDEGYGPEAQKAVSAFKVQVGKSGRFVGQNA
                     VQLHGGMGMTEELNVGHYFKRLTMIDTLFGNVDFHLKRFGSL"
     gene            complement(12700..13893)
                     /locus_tag="AC16PP_10570"
     CDS             complement(12700..13893)
                     /locus_tag="AC16PP_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6482113.1"
                     /translation="MRFTEDDLAFQAEVRSFIDRHWPADVRLARATVPAHGLDRPAEE
                     QAWFDALVARGWSVPNWPLEHGGTDWTPTQRFIWDRETVGAETPQMSVFGVTMLGPIL
                     CRWGSVDQQEKFLPPIREARVQWCQGYSEPGAGSDLASLKTTARLDGDHYTVDGEKTW
                     TSGAHVADWMFCLARTSSTNKPQEGISFLLIDMRSPGVEVHPIEILGGQHSVNSVRLD
                     GVRVPVAHRVGEENQGWTYAKALLSHERTGVARVATSGVQLNRVREIAEGQEPSTDGL
                     WQDAGFQRRYRELEIELIGLEMLELRILADLEAGQVPGPESSILKIKGTEIGQRINEL
                     MVEALGYYALPYPDELLIDNEGPVGHDHALAALKGWLFSRAWSIYGGSNEVQKNIIAR
                     RALGL"
     gene            14092..14715
                     /locus_tag="AC16PP_10575"
     CDS             14092..14715
                     /locus_tag="AC16PP_10575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6482114.1"
                     /translation="MQDDALPPQLFARADESPDSAFYREPRFVTHIDDATIDCLTAFY
                     RTFIPENAAVLDLMSSWVSHLPPECSYARVAGLGMNEAELAANPRLDDHVVHDLNAAP
                     ELPYPDSAFDRVILAVSIQYLTRPVEVLREALRTLREDGEICIAMSHRLFPTKAILAF
                     HRLGPADRVRLVSTYLELAGFLDVRFVDRSPDGADPLWLVVGRRAGR"
     gene            complement(14717..15379)
                     /locus_tag="AC16PP_10580"
     CDS             complement(14717..15379)
                     /locus_tag="AC16PP_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482115.1"
                     /translation="MAETEAYTFTAALTVCLDITQPESYLAVPGVRELAADLDMDVRW
                     LPYSVPPWPARSEPGPEADRGTWHRWHRARYRERDLRRYARLLSIDLGDLHRQIKGEL
                     AGLALLWVQDQPAAVGGALLDGLLEGHWSGALNIEEPASVTAMLVQAQARVAGWDEYR
                     SGRGPVELAALRSALSAAGVFDVPSLVVAHAVPEPEVFLGRAHLPMVRWLLTDRQGAP
                     PI"
     gene            complement(15372..16088)
                     /locus_tag="AC16PP_10585"
     CDS             complement(15372..16088)
                     /locus_tag="AC16PP_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR6482116.1"
                     /translation="MPSTSSFDPLGSSSGLIVYVDFKSPYAYLAKDPTFQLADDLGID
                     LDWRPLTLDINSYLGSARLNASGAVAESRRTPEQWHRVRYAYRDARRYANLVGLTVRG
                     TTKIWDSSLASIGLQWAKGQGAGPLRAYIDLTYERFWKRELDIEDASVVAGVLAEAGA
                     DPDGFVDFARGEGRAEHDVMQQRIFAAGIFGVPSYVLDGELYFGREHLPRIRWLLTGR
                     PGPCPDVAYRHFDAQETIRG"
     gene            16213..17070
                     /locus_tag="AC16PP_10590"
     CDS             16213..17070
                     /locus_tag="AC16PP_10590"
                     /inference="COORDINATES: protein motif:HMM:NF006109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR6482117.1"
                     /translation="MAYNTILYDVEDGVLTLTLNRPERLNAFNHEMLSEMIDAFDRAD
                     ADDEVSSIIVTGAGRAFCAGADLGAGGDTFNADGREDRASGLHPDGGGRLTLRIYELK
                     KPIIAAVNGPAVGVGVTMTLAMDIRLAADVAKFGFVFARRGIVPEACSSYFLPRIVGI
                     SQALEWCYSGRVFPAEEALAGGLVRSLHSKEELMPAARALACEIADNTSAISVTLVRQ
                     MMWRMLGADHPMEAHKIDSRGVYFTGKSPDAREGVEAFLEKRPARFPGKVSSDLPEFF
                     PWWEERTFS"
     gene            17081..17320
                     /locus_tag="AC16PP_10595"
     CDS             17081..17320
                     /locus_tag="AC16PP_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HIR6482118.1"
                     /translation="MSSPTIAQKAPYAVDVQAGRKYFWCACGRSAKQPFCDGSHEGTA
                     LTPLPYTAEKDATLYFCGCKHTKNVPLCDGSHNDL"
     gene            17336..20125
                     /locus_tag="AC16PP_10600"
     CDS             17336..20125
                     /locus_tag="AC16PP_10600"
                     /inference="COORDINATES: protein motif:HMM:NF015926.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HIR6482119.1"
                     /translation="MLSRIFGNKDPLSQPDPAARRRAVEGLSEGKAGELQERLLELAR
                     SDADPGVRQACVSRLQGTSDLEQLLDDGLMANRAAERIAELLGPDASSELTRHPLVLR
                     ARLFRLAPEDAARLIDSIEDEDTLVDLTAGSREPLRGRILAGIRSARALAALEQQSRG
                     RDKGLNRFAREGMERIKQLRREAQATLDRLNELADALERASRQAPGIAARQRYESLLR
                     EFDQAYERFAGHARALEGCNEPPRDASPIRARVAALVAPAEPVAISRQEETPASGGEA
                     FADLVPQLEALEGLMEAGHDFQTVSETRSRLTEAWLRAADHALPGDAEHRVFERVSHA
                     YQNLADAAGRLTAAGWQTHRDPLPSDLPDDPGEAAGFWQAAEERRRLLKRAEGLVRAV
                     AWPDWASPTAELRDLLDDVERLREEVARSDALGSSRHEDLKMLISTAASAIDEGAVRA
                     ATEALGRARAILQALPARGREAQARALQQESARLSELRDWQTFATTPKREALCEAVQN
                     LADQPLDPPDQAERIKALRGEWNGLGPVSGREDRALADRFNRLAEQAFEPCRAYFAEQ
                     ASVRASNLVERGKICDQLENYLESTDWKAADIKAAERILRAARDEWRRFQPVDRNPGK
                     PVEERFEVLQERLHKLIKAEWDANLQLKRQIVEEAEALAGSEADPHDRAQEAKALQRR
                     WREIGITPRGPDRKLWQQFRGACDAIFDALGAAREASHQAVSEALDAADNLLTSFESD
                     LGNTSATEAEAEILTGFRRRFSELPPLPEREGRQISRRFEELTRSYRSLLHQKSRYQE
                     RAELDRLRALDEAVTHTEIAPGLTAEPTQDAIADPVVSERLQKSGEPIPMDALRALTV
                     RAEILAGAESPAQDREIRLKLQVEEMNVGMGSQRRAQDPLEMAREWCARGPKDEACSA
                     LRERFFDALARALED"
     gene            complement(20122..>21409)
                     /locus_tag="AC16PP_10605"
     CDS             complement(20122..>21409)
                     /locus_tag="AC16PP_10605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6482120.1"
                     /translation="VLGTSLALAGVVLDRSFQASVRAGAEEQLRLIIYSLMGAVVEGN
                     DDIRLNPDLPEPRLNQPGSGLYAVVDRGDGGDRWRSPSVTTTGVRFPQEAVPMSAGEF
                     RFESARQGLVDRFFLSYAVIWEDADEALLTFRVGTDQAPFQAAVQGFRRNLYIGLGAV
                     TVLFVVAQFFAVRWGLIPLRTMAEEVREMEEGHRERLSRGFPSELQGLAENLDRFVAH
                     EQRSRSRYRKAMEDLAHSLKTPLAVIRNALAEPDSSTPSLLSDQLDRMESSVTHQLSR
                     AAVTGPVVVGRSVNLATVVSRLVRALKTAYADRGIEVAEALPENLVVRGDERDLMEML
                     GNVLENAFKYTTARIRIGAERNSVVSVWVEDDGPGIAPELRQEVLNRGSRADEMRPGQ
                     GIGLSVVAELVALYQGELRIETSALGGARICLDLPA"
BASE COUNT         3872 a         7241 c         6932 g         3364 t
ORIGIN      
        1 ctccacgacc tgtacggcaa ggctttcgag gcccgctacg aggcgtacga ggagatgacg
       61 cgctccgggg ggatcaagct cttcaagcgg atccgggcac aggagctgtg gcgcaagatg
      121 ctctcgatgc tgttcgagac gggccatccg tggatcactt tcaaggacgc ctgcaacgtg
      181 cgttcaccgc agtcccacgt tggcgtggtg cactcttcca acctctgcac ggaaatcacc
      241 ctgaacacca acggcgaaga gatcgcggtg tgcaacctcg gctcggtgaa cctggtgcag
      301 cacgtccgcg acggggagct cgacacgcgc aagctcaagc agaccgttcg caccgcggtg
      361 cgcatgctcg acaacgtgat cgacatcaac tactactcgg tgccccaggc gcggaactcc
      421 aacatgcggc atcgccccat cgggctcggc atcatgggtt tccaggatgc tctgtacaaa
      481 ctgcgtatac cttacgccag cgaagatgcc atcaccttcg ccgaccgttc gatggaagcc
      541 gtcagctatt acgccattga cgcttcctgc aagctggcca aggagcgcgg tgcctacgcg
      601 agcttctccg gctcgctgtg gagccgcggc atcctgccca tcgattcgct gaagcaactt
      661 catcaggaac gtggcgccca gtacctggat gccaatttcg acgccaccat gaactgggac
      721 aagctgcgcg gccgcgtgca gatcaacggc atgcgcaact cgaacgtcat ggccattgcc
      781 cccacggcaa ccatcgccaa catcacgggc gtctcccagt ccatcgagcc cacgtatcag
      841 aacctgtacg tgaaatcgaa cctgtcgggc gagttcaccg tggtcaaccc gtacatggtc
      901 cacgacctga aagaccgcgg attgtgggac aaggtgatgg tcaacgatct caagtactac
      961 gacggcagcg tgctgtccat cgaccgggtg cccgacgagc taaagcagct ctacgccacg
     1021 gccttcgagg tggaaccgag gtggctggtg gacgccgcga gccgccggca gaagtggatc
     1081 gaccaggctc agtcactcaa cctctacatc gccggagcgt ccggccggaa gctggacgtg
     1141 acctaccgaa tggcgtggct cagagggctc aagaccacct attacctccg ctccctgggc
     1201 gccaccagca cggagaaatc gaccctcgac cgcggcaggc tcaacgccgt gtcggccacc
     1261 caggccgacc cgaccaccgc cgctcgagcg ccgacaccgc tcgacatgga cagcgtgact
     1321 gccggagggc ctgcccaggt cccgctggcc tgctccatag acgacccgga ctgtgaagcc
     1381 tgtcagtaaa ggagactcga aaatgctgag ctggaacgac ttcgaagaag aacacgcgaa
     1441 cgcggagaca gcgatgcagc aaccgcccgc cgaggctccg ctcgagtcga aggaagacgc
     1501 ggccgcatcc ttcccgcccc caggcgaggc tgtcgcgggc gcgggcgctg tcgcggaggc
     1561 agctgcgggc gcagctgcgc ctgccccgag gcccgtcgag gagttgccgc ccatcacggt
     1621 cagcgagccg gccgcgccct tgtccgaagc gccgagtaat gatgaggtgc tgcagcgcgc
     1681 ggaagccgcc attggcgaga tgggcctgga ggggctcgcc gaggatggca ttgaggagaa
     1741 ctatcagcgg gtcaccgtgg atcagaaacg gatgatcaac tgccgcgcgg atctcaacca
     1801 gcttgttccg ttcaagtacg actgggcctg gcagaagtat ctggacggct gcgcgaacca
     1861 ctggatgccg caggaaatca acatgaccgc ggacatcgct ctgtggaagt cggagtccgg
     1921 gctcacccag gacgagcggc tcgtggtcaa gcgcagcctc ggattcttct cgaccgccga
     1981 ctccctggtc gccaacaacc tcgtccttgc gctctaccgg ttgatcacca atccggagtg
     2041 tcgacagtat ctcctgcgac aggcattcga agaggccatc cacacccacg cgtaccagta
     2101 ctgcatcgaa tcgctgggga tggacgaagg cgagatcttc aacatgtacc acgaggttcc
     2161 tgctgtggcc cgcaaggccg gctgggcgtt gaagtacacc cgggacatct cggacccgac
     2221 gttccagacg ggcactcccc aggcggacaa ggcgctgctg aagaatctga ttgcctatta
     2281 ctgcgtgctg gaggggatct tcttctactg cgggttcact cagattctgt ccatgggacg
     2341 cctcaacaag atgacgggca cctccgagca gtttcagtac atactgcggg acgaatccat
     2401 gcacgtgaac ttcggcatcg atgtgatcaa tcagatcaag ctcgagaacc cggggttgtg
     2461 ggacgaggaa ttgcagactc tcgcccggca gatgatcctc gagggtaccc agctggaaat
     2521 cgagtacgcc aaggacacca tgccaagagg aatactcggc atgaacgcga acatgatggc
     2581 cgagtacctc cagttcatcg caaaccggcg tctggcgcag atcgggcttg ccgagcagtt
     2641 tgcgggagcg aagaacccgt tcccgtggat gtccgagatc atggatctca agaaggagaa
     2701 aaacttcttc gagacccggg tcaccgagta ccagactggc ggggcgctca gctgggattg
     2761 agggcagctg agggcgccgc cgccctcagc gaacgttgac gcgaagcggg atcgtgggtc
     2821 ccggtctttc ggtcatcacg acgctgtaaa cccgctcgtc ccatcggtag tacacgcggt
     2881 aaccgtcctg gacctccacc ttgcgtcgct gcgtgtggca atcggcccgc agatcccagg
     2941 ccagcagagc gaccagatcg gcttgcccga tcggcttgac gggcgcgcag tcgccgctgc
     3001 gttcgaccac caccgacgag gcggtgagcg gaatcactcg aacgacgtcg ccctctacct
     3061 gcatttcccg ggcagccgac gacgcgctca ggaacatcag catcccaaag accaatccac
     3121 gcatccccgc catcgtcaat ctccccgtac cggccaaccg agccgttcca gcacgttcag
     3181 atcggtaagg ctcagcccac cggccgcgat gcgcatcacc acggcaaacc cgccttccgg
     3241 ctcggcgcgc gtcacgaaat cggcaccttc aacgcgctcg tccagcgaac gggcatagcg
     3301 gcgggcacga tgcgccgtat cgaagcgcac cgcgtgctca gcggctcgcg tcgcagacgc
     3361 ctggcgcgct ccggtcttcc ggacgatgcg ctcgcgatgg ccctgcaccg tcgagacgta
     3421 cctcagggcg ccggccggca gcgtgctgct ggaggctatc ctgctcgggc cgtagttgta
     3481 ggccgcaagc gcccggtcct cggcgccgcc gcttcgcgcg agcagctcac tcagatattt
     3541 cgcgccgcgc tcgatgttca gacaggggtt gtacagctcg gagagtcggc cgacaccgag
     3601 atgccgggcg gtgcccggcc actggatctg catgatcccg tgggcgttgg cagcggatct
     3661 ggcatccgga tcccagttgc tctccgtagc tgccacggcc agcagcaggt ccacaggcac
     3721 gtcgtgcagc cgggaagccg cctcgaaaca ggaaagatac ggatattgcg tgaccgcatg
     3781 accggtaccg cagagcagca gagcggccag caggggcgcg gcgcggctca tggcgcaccc
     3841 gtcccgatga gcggccgcac ctgctcgaga agccggcgcc gctcctcctc gtcagcgtac
     3901 gcgaaaaaca cctgataacg accgggtccc cgacgatcta tcccgaaggg gtgctccgtc
     3961 tcgctgctca agcgcctggc aaaaccttcc gcggacgtct ggctgcgaaa cggcgcccag
     4021 acctgctgga tggtttcgga agcggaccct gcaggcgggt ccgcagcggg cagcaagcgc
     4081 acggcaggcg ggagcggttc cgggccttcg gccggcggga gcggttccgg gccagcaagc
     4141 cgcaccatgg gctcctgcat gtcggcggcc agcggcccct cggcaacctc ggcgggaccc
     4201 gcgcccagac tctcagcgtc cacttcggcg tcgcccgcgg cgggatcggt ctgcgcgggc
     4261 agggcggtcg gctggtcctc gcgggcagcc cagcccggct cgaactggcc ggccgcggcg
     4321 cgcataccgg tcagccaggc acccaggctc gagactcccg agtcgggccc gtcgactgcc
     4381 aagcgttgcg cagccaccac gaccatcgcg ccgcccaaga tcagtccaag caagaatttc
     4441 atgacgcctc ctcagatcct cgttatgccg ccggggccgc cgcgaaagtg ggcggcgcgc
     4501 caatccacgt cgccgccata ctcggcgaga acgcggccca acgcctggag ccactggccg
     4561 gtgctttccg ggacgccgcc aggacaggct ccgggtccgg cgttgatctg ccgcgcgatt
     4621 cggtgggcga cgctgcgctt ctccaccgca agaacgtaaa cgcccgcatc cgggcggctg
     4681 gccgtcgccg ccccggaggg aacccggatt cggtatcgcc ggggcccggg ctcggcgata
     4741 cggaggctgc gtccgcagac ctgagattcc agacgctcat cgcgactgga gacgaccagc
     4801 aggtaggccg gacgcagcag atcgaaagac acctcgatgc agccgccggg tccggtcgac
     4861 gactggcaga cgccccggct gcgcgcggtt tcgtagctca atccggaaag cagcgcagcc
     4921 ggcatgccca gcggcgtcac cgggccaggg cgctcgctgc gcggcctgga tatcaccacg
     4981 gaagcgatct gctgagcgga gccctcccgg cgcaggtcac gaagggtcgc gacgagctcg
     5041 tctgttccgt tgcccagcgg tctcgccgtc agcgccatct cccagctcgc ctgggcgcga
     5101 tcaatggtca acgcggccag aggccggacg ctgagctgcc gggtgagtgc ctgagcgacg
     5161 ccggacagat cggactccac gggcatggcc acgtagagcg agccgtcgag cccggcagga
     5221 atcgcgcagc ccaggcccgc cagcatgagc tcgccgatga cgctgctgtc gctcaaaggc
     5281 agcgggctct gcatcgaccc gggcggaccg ccgttcgctc gggtaccgag cgccgtccgc
     5341 tccgttggga gcaggcggcc cttccaggcg aggctgacgc cgctgaccca gacgccttcg
     5401 tccacgtcga ccatggcgat gttgaggtga tagatgtccg ggccccgctg ctgaatctct
     5461 atgcccagca gataggggac ctcctcaggc gcagcacagg gctgccggtc gcgctcccag
     5521 gcgattcgca cccctccttc gcggatgagg tgacgggtca accgctgctc gaccgcctga
     5581 acgagccggt tgccggccgg ttccgcgcga ccgtccagca tgggcacgaa gcgtacggtc
     5641 tctcccttga acttggggtg gcgggcgagg gtctcggcca gctccggacc cgccgtttcg
     5701 tcgagccatc tgcccaggga tccgccgagc agctcgccgc caggcgccgc ggacgccaga
     5761 cccggcagag tcgccagcag tagccacgtc gccaggcacc tagctgccga catgtcgcac
     5821 ctgcgcatat cggtcgatga tcagctcccc aatcacgtcc tggtcgatcc agctgcggat
     5881 cgacgcttcc agctcgaccg gcaggcgcac gccccaggaa aagccgcccg ggttcccggc
     5941 tgcccgggcg agggaagcgg tcacgcgcga cggtatggtg tctgcctgga gctcgtagac
     6001 gcggcctggg ggcggggagg cgaagaactc caggttccgg ccgaggccga gcgcggtgcc
     6061 gcccggatcg tccggctgaa aggcgaatac acggacgcgt cccgtctcga tgaggcgcag
     6121 gaaatcccga tccgaggcaa agcgcaacgc gagcccatgg ggagcggcag gttcgcccgg
     6181 taccttctca acgtcctcaa cgtcctcaac gtcctcaact tctgccggcc tctcctgggg
     6241 cgttcccgtc gccaccggcg gcaccggttg cgggcgcgcg gggcgcacgg cagcgcgagg
     6301 ttcgacctcc ggggccgcaa gttccgacgc ctgcgccatc ccgacgggtt gctgcaccga
     6361 cggcgcgggc cgcggatcaa ccgccggctc ggcgtttttg acaagggcga gcacggctat
     6421 gaggcaaaag gcaatgatcg ccatgaaccg catgacgtca gtctgcaggc catcgccgtc
     6481 ggcggcgtga ttgaccggct cgaacgtcat gccaccaccg ccggttcctg ggggtcgaga
     6541 cgggccagac gggcctcgat ccgctcgcca aggcccacca gctcgcgaag gtctgcacgg
     6601 ctaacgccgg agtagaaggt ctgcaggcgg gcgccgacca ccatcgtctt gaccagacgc
     6661 atgacgtagc ctccgacccc gccgacgatg gtcgtcgtca acgccagcag aatacctccg
     6721 tcgacgagct tgtgaaggac cgagcctgcg gagcccgaca gcgcgccgcc gggatcacca
     6781 agggccgcct gcagcgcgct gcgcatgccg acggccgtcc atatgacccc gataccgacg
     6841 aacaggttga tccagacgtc ggagagctga tcgagcttga cgaccacctt caggtccggg
     6901 ccgccggcga gcagtcggct cagccggtgc aggttggcgg catagaccgt cagcaccaga
     6961 gcgaagagcc aggcggagtg ccccaggttg agctgcagcc agtcgctcac gggtgcagcg
     7021 tccgcggcaa cagccccgcc cgagaggccc agggccacca gcgcgagccc cgcgcccgcg
     7081 cccgctccgc acacgaactc cgcgacgcct ttcattcagc gcccgtccgc gccgcaagcc
     7141 cgacaggtcg cttccaggac gagatcggct tccttgagca gggtgtccgc ccggtagaag
     7201 ctcgcccggt ccgcgctggc ccgcatcagg ccaagctcct tgtcgtgcag ctccgcgcgc
     7261 atgtcggaca tgacctgatc gcgcatgggg caggtctgcg agcacgtgtt gtagtcgccg
     7321 cgcagggaga cgaacttgac gttgaagtaa cggttgtaga gcaactccgc ctggcgcttg
     7381 ctgatgacgc ccatgtcgtt cacgcgcatc aggtgatcgc tgaagagcat gggattcctc
     7441 gagtccggat tttccgcggc gatcgccagc agggactgaa agtagccatc gaagcggtac
     7501 tcgcatccgt tctccagccg atcctcggcg aggctcatgc tgcgctcgag gtcgtcctga
     7561 aatcgcaccg cacaggcatc cacggtctcc gtgctggtgc agccagcgac gagcgagatg
     7621 aaaaaggccg gaacaaatat cctacgaaac atgtctttct cctgatcgaa tcggcgctac
     7681 atggccagcg ggctgacgaa ctcggactcg ccgatggcgt aggggggcgc cgcaatgggg
     7741 ctgtcggtgg aagcctcgcc gaacaggatg cccttcacgc cgtcgaccac ttcggtcatc
     7801 tccggcgtga tcagcccgaa cgactggttg agctgctcca tcagcgccgc gcgtccgatt
     7861 tcctcctggg tcgcggcgat gaggcgggaa gcttcctcca tgtccagata gatgtcggcg
     7921 gcgaccaccg ccagcgagcg gccacggccc tcacgctggg tcgcgaccag ggcgtagtac
     7981 tgtgcaaacc tgtcggaaag ccgcaccccg gaacggccgc gaagcgccgg ctgctcggcg
     8041 gcggcgctgc ttgcaggcgt ggtccccagc agggcgttct gcagcgatgt gggggtggtt
     8101 cgatgcccga gctcttcccg caggcgcttt tccaggctcg cgcgggtatt gtttacggcc
     8161 agttccatct ccggaagccg gtcacccatg atggtgagca tctccagata ggtcgcgcca
     8221 agctgtttgg ccatgcgctc gcagccctgg tcgccgtcgg agccctcgca gaggctttcc
     8281 acgtatagct gatgctgcag atcgagccgg ttgatgacct cctgcaggct gttctccatt
     8341 tctttggcaa cctggcctgt ctcacgtatg tcctcgagca cggttgtcgg gaagagccga
     8401 acgaccgggc ccgaatccgg gacggcggaa gccgtggcgg cggcaagcag caggaacggc
     8461 agcagcacgc ggccgagcgg gctgccgccc tttcttgggt tggtatccat gggcatatcc
     8521 tctttgcttg cgcgccgcaa aacggcgcag gtgtctggac ggaagagagc gctcaccaat
     8581 ctggctcccc gacggcggga gtgacctgaa cctgaacgcg cacgcgatcg cccgggtctg
     8641 aagccaccac cttggtgaac tcttctccct gatagacgta gcgcacccgg tagccggcga
     8701 cctcttccac ctcgcgggac gtcatgaccg tcgtgcagcg ccgcggcgtg tagccggcat
     8761 ggcgcgaccg ggaccggctg acgctgctgg caaccgaggc ccccgccagc gctccggcaa
     8821 cggtcatcgc cttcttgccg ctgccgctgc cgaactggct gcccagcagc ccgcccacga
     8881 cgccgccgat cacggcggca accggactgc cgtgatcgtg gtgatcctgg tagtggtcgt
     8941 gggcgtccgg atggccaggt ccggtgtatc ggtgcgaacg ggtgccggaa ctagccgcgg
     9001 cgtaccccgc aacccgacaa tcccttcggg gctcggtgac cgtgcgatat tccaacaggg
     9061 gctccacgcc gacgacgtcg gcgtaggtgt agtgagtgtg ggatccggcg ggggcggctt
     9121 gcggaaaacc gtcgctgtgg cccgccatgc cgggtgccgc ggcaacgcag agactcaagc
     9181 cgaggccaag ggctggtgcg cgcatgtttc ctccggaact cccaaatctg tcgagactcg
     9241 aatgttatgg gagttcagct aaacagaagc tgaacggggc ggcccgaccg gggcgggttt
     9301 gccctaccgg gcgcacccgc ttctccagcc ccccggagcg gcgtatgctc atccagactc
     9361 cagcggcacc gctcgcagga cccggccgga gagccaacat gaccaagtca gaccatcatc
     9421 agatcctgat catcggcggc ggggcggcag gaattacggt tgccgccagc ctcaagcgca
     9481 gacagggacg gcacgacctc gacatcggaa tcgtcgagcc gtccgagcat cactactacc
     9541 agccggcgct gacgctcgtc ggcgcgggca cttaccctct cgagaagacc cgccgggcgg
     9601 agtcgcggct gatcccgcca ggcgtggcgt ggcatcggga ctcggcaacc ggtttcgatc
     9661 cggagtccaa ctcggtgacc ctggcctccg gggcgaggtt gtcctacgac tacctggtgg
     9721 tctgcccggg cctggagctc aactgggagc gaattgacgg tctccgggaa accctgggga
     9781 gcaacggcgt ctgcagcaac tactcgcccg accacgtcga gtacacgtgg caatgcctgc
     9841 agtcacttca ggaaggcgcc accgcgctgt tcactcaagc gccactgccg ttcaagtgcc
     9901 cgggagcgcc tcagaagatc gcctacctgg ccgccgatca tctgcagcgc cgcgggctgc
     9961 tcggaagctg ccatctgcac tttctcaccc acggccccgc tctgtttgcg gtgccgctgt
    10021 tcgcagcgga gctgagcaag gtggccgagc gctggggcat cgacgtccat tttcagacca
    10081 acctgatcag ggtggacggg cccgcacggc aggccacctt cgaaaaggtc ggcggcgaag
    10141 gagagggaga agaggtcacc atgaccttcg acatgctaca cgcaaccccg tatcagagcc
    10201 cgcccgcggc cgtgaaggag agccccctgg ccaacgaggc aggctgggtc gacgtgcacc
    10261 agcacagcat gcagcacacc acctacgcca acgttttcgg gctgggggac gcctgctcga
    10321 ccccaaactc caagacggcg gctgccgtgc gcaagcaggc gccggtggtg gtgcgcaatc
    10381 tgctccacct gctcgacggc ggcagcgtcg aggaagctta cgacggctac ggctcgtgcc
    10441 ccctgaccac cgcgtacggc aaggtcctgc tggccgagtt cacctacggg ggaaaggtta
    10501 cgccgacctt cccgctggac ccgcgcaagg agcgaagaag catgtggtgg gtgaagaaga
    10561 cggcgctgcc gatcatgtac tgggactaca tgctgcgagg tcacgaggcg ttcccgggcc
    10621 acgacacgga gtacgaagcg ccgccggtgt gagcgggcta gggcgagttc aggcgagaag
    10681 cgtcgccagc atcttcctca gctcggcgac gaacgccagc ggctggtcca ggaacagatg
    10741 gtgctgtgct tccggcagcg ccacggccgg gaagtcctgg gcaatgagct cgcgcatgta
    10801 gtcttccgtg cgagccgaga acagctcgct ttccgctccg tagatgaggc ccgtgggcac
    10861 gtccagatgc cggtagtctt ccggcgtgcg ctcgacatcc ttcagagtcg acggcaggtc
    10921 ctcgtcgaac ttccaggccc atccgccatc cacgggcatg agcgaatgac gtgcgatgta
    10981 ctcgagaata taggcgttgt cgcagctctg cgggggctgc acgcgaaagc ggccgagcgc
    11041 ggtctcccgg tcgggataca cctttgaccg ccctcccatc gccggcctgt ccggaaccgg
    11101 ctcctccgga tgacggattc cggaatccac caggaccagt gccccgaaac gatcgggaaa
    11161 gagcacggcc gctttgacgg ccatgtaacc gccgaagctg tgcgccacca gggctgcacg
    11221 ctcgtcgaac cccgcatggt cacagacagc cctgatctcc tgggcgtagg tggcgccgtc
    11281 gtactcgtag cggtagtcgg agtcgcccat gcccgtgagg ttcatggcgg cgacccgata
    11341 gccgtcgagc agctgaggcg cgatgaagtc ccaccagtgg gagtgggcgt tcatgccgtg
    11401 gatcagcagg atcccgggtt tatcctcgtc gccccatagc tggtacacca cgtcgcactc
    11461 atcgacctcg actgaccggg tcgcgtactc ggtctccacc gcctcgaaga accagtcggg
    11521 tatggaggcc ccggagtcac gatcccagcg agccacgatc taaagcgacc cgaaccgctt
    11581 gaggtggaag tccacgttgc cgaacagcgt gtcgatcatg gtgaggcgct tgaaatagtg
    11641 gcctacgttg agctcctcgg tcattcccat cccgccgtgc agctggacgg cgttctggcc
    11701 gacgaacctg cccgacttgc ccacctgaac cttgaacgcc gatacggctt tctgcgcttc
    11761 cgggccatag ccctcgtcca ggcgcatggc cgccatgtac atcagagact tggcctgctc
    11821 gtgctccatg aacatgtcca ccatgcgatg ctgcagcacc tgaaacttgc cgatgggctg
    11881 accgaactgc tcgcgggttt tgcagtactc caccgtcgtc ttgtagagca cctccatgca
    11941 gcccacggct tccgcgccga cggccaggat ggcctcgtcg acgacctgct cgagaatggc
    12001 gagccccttt ccttcctcgc cgatcaacgc atcgcttccc acccggacgt tctccagcgt
    12061 cacctcggac gcccggaagg catctaccgt ggggtagtcg cgcctggcca cgccttcggt
    12121 atccgcaggc agcacgaaga ggctgacgcc atccgcatcc cgctggccgc cgcccgtgcg
    12181 gcaggatacg accagaaagt cggcggacgg gccgttcata accacggctt tgtaaccgtt
    12241 gatcacgtag ccgtcaccct ccggggacgc cgtggtggtg agatcggcaa ggttgaatcg
    12301 tccctggggc tccgcgtacg ccagggcacc ctgcagcgat ccgtccatga cgccggcgag
    12361 gtatttctcc ttctgcgacg ggctgccgcc gatcttcagc gcgcccccgg cgagaaccac
    12421 cgtcgccagg aagggctcca cgaccagccc tttgccgaac tgctccgaca tgatcatcga
    12481 ctcgatggcc gttccgccga agccgccgtc ggcttccggg aacgggaccc cgagccagcc
    12541 gagctcggca aacgtccgcc agttgtcgct ggagaaaccc tgctcctccc gaaccacccg
    12601 ctggcgcgcg tcgaacgcgt agtcgttctg gatgaacttc tcgatgctgt cctgcagcag
    12661 cgtctgctcg tcggaaaaag aaaaatccat aacttcccct caaagaccca gcgcccgccg
    12721 ggcgatgatg tttttctgaa cttcgttcga cccgccgtaa atgctccagg cgcggctgaa
    12781 cagccaaccc ttgagcgcgg cgagcgcgtg atcgtgacct accgggcctt cgttgtcgat
    12841 cagcaactca tcgggatagg gcagcgcgta gtagccgagg gcctccacca tcaactcgtt
    12901 gatgcgctgg ccgatctccg tgcccttgat cttgagaatg gacgactccg gccccgggac
    12961 ctggcccgcc tcgaggtctg caagaatgcg caactccagc atctcgaggc caatcagctc
    13021 gatttcgagt tcccgatagc gacgctggaa gcctgcgtct tgccagagac cgtctgtgct
    13081 cggttcctgc ccttcggcaa tctcccgtac gcgattgagc tgcactccag acgtcgcgac
    13141 ccgggccacc cctgtccgct catggctcag aagagctttg gcgtaggtcc agccctggtt
    13201 ttcctcgccc acacgatgcg cgaccgggac gcggacaccg tccagccgga cgctgttcac
    13261 gctgtgctgg ccgccgagga tctcgatggg atgaacctcg acacccggcg agcgcatgtc
    13321 gatgagcagg aagctgattc cctcctgggg cttgttcgtc gacgaggtcc gagcaaggca
    13381 gaacatccag tccgccacgt gcgcgccgct cgtccaggtt ttctctccat ccaccgtgta
    13441 gtgatcgcca tcgagacgcg cggtggtctt gaggcttgcc aggtccgaac ccgctccggg
    13501 ctccgagtag ccctgacacc attggacgcg ggcttcccga atgggcggga gaaacttttc
    13561 ctgctgatcg acgcttcccc aacggcacag gatgggtccc agcatcgtta cgccgaacac
    13621 gctcatctgg ggcgtttccg cccccacggt ttcgcgatcc cagatgaacc gctgggtggg
    13681 cgtccagtcg gtaccgccgt gctccaaggg ccagttcgga acgctccaac cgcgcgccac
    13741 gagcgcgtcg aaccaggcct gctcctcggc ggggcgatcg aggccgtggg cgggcacggt
    13801 ggcccgggcc aggcgaacgt cggcaggcca gtgccgatcg atgaacgaac gcacctcggc
    13861 ctgaaaggca aggtcgtctt cggtgaatct catgtccatg gtggtcccgc gaaagtcccc
    13921 gagatgcgaa accgcggaat cttatcagct cctatctgcg cggtcttggg gcccggatca
    13981 tcgggtaacg gaggccgggc tcgcctccga ggacagggca ggccgcgggc aacatgccat
    14041 tggccgcatt cgctagaatg acggcccaag cagcaacggt tcggatccac cttgcaggac
    14101 gacgcccttc ccccgcagct tttcgcccgg gccgacgagt caccggattc ggccttctat
    14161 cgcgaaccgc gcttcgtgac gcacatcgac gatgccacca tcgactgcct cacggccttc
    14221 taccgcacgt tcatccccga gaacgccgcg gtcctggacc tgatgtcgtc ctgggtatcc
    14281 cacctgccgc cggagtgcag ctacgcgcgc gttgccggtc tcggcatgaa cgaggcggag
    14341 ctggcagcca acccgaggct cgacgaccat gtggtgcacg atctcaacgc cgcaccggag
    14401 ctgccgtacc cggacagcgc gttcgaccgc gtcatcctgg cggtttcgat ccagtacctg
    14461 acacgccctg tggaggtact gcgcgaagcc ctgcgcaccc tcagagagga tggcgagatc
    14521 tgcatcgcca tgtcccaccg cctgtttcca accaaggcga tcctcgcctt ccacaggctc
    14581 ggccccgccg accgcgtgcg gctggtatcc acctatctgg agctcgccgg ttttctggac
    14641 gtgcgcttcg tcgaccgatc ccccgacggc gcagatcccc tgtggctggt cgtcggacgt
    14701 cgcgccgggc gctgaatcag atgggcggcg cgccctgccg atccgtgagc aaccagcgga
    14761 ccatcggcag atgcgcgcga ccaaggaaga cttcgggctc gggcaccgcg tgggccacga
    14821 cgagcgaagg gacatcgaaa acccccgctg cgctgagcgc ggagcgcagg gctgccagct
    14881 cgacggggcc gcgcccgctg cgatactcgt cccagccggc aacgcgggcc tgagcctgga
    14941 cgagcatcgc ggtcaccgat gccggctcct caatgttcag cgccccgctc cagtgaccct
    15001 ccagcaggcc gtcgagcaaa gcgccgccca cggcggccgg ctgatcctgc acccagagca
    15061 gcgcgagccc ggcaagctcg cccttaatct gccggtgcag gtcccccaga tcgatgctga
    15121 gaagacgggc gtagcggcgc aggtcccgct cccgatatcg cgcacgatgc cagcggtgcc
    15181 aggttccccg atccgcttcg gggcccggct cggaccgtgc aggccaaggc ggcaccgaat
    15241 agggcaacca tctcacgtcc atgtccagat cggcggcgag ctcgcgcacg cccggaacgg
    15301 ccagatagga ctccggctgg gtgatgtcca ggcagacggt gagcgcggcg gtgaacgtgt
    15361 aagcctcggt ctcagccacg gatcgtttct tgcgcatcga agtgacgata ggccacgtcc
    15421 ggacaggggc ccggtcggcc tgtaagcaac cagcgtattc tcggcaggtg ttccctgccg
    15481 aagtagagct cgccgtcgag cacatagctc ggcacgccga agatgccggc ggcgaagata
    15541 cgctgctgca tgacatcgtg ctcggcacgc ccctcgccgc gggcgaagtc gacgaacccg
    15601 tcggggtccg ctcccgcctc tgcgagcacg ccggcaacaa cggatgcgtc ctcgatgtcg
    15661 agctcgcgct tccagaaccg ctcgtaggtc agatcgatat aggcccgcag cgggccggct
    15721 ccctgccctt tggcccactg cagaccaatt gatgcaaggg acgaatccca gatcttcgtg
    15781 gtgcccctga cggtaaggcc caccaggttc gcgtaacgcc gggcatcgcg ataggcgtac
    15841 ctgacccggt gccattgctc gggcgtacgg cgactctcgg caaccgcacc cgaggcgttc
    15901 aaccgcgcag agcctaagta gctgttgatg tcgagcgtga gcggccgcca gtccaagtcg
    15961 atgccgagat cgtcggccag ctggaatgtg gggtccttgg ccaggtaggc ataagggctc
    16021 ttgaagtcga cgtagacgat gagccccgat gagctgccga gcgggtcgaa agaggatgtg
    16081 gacggcattc gggcgattgt agtaggggtt gcgcggcgcg ccaaaccagg tcgcatgcca
    16141 gtccgcatgg aaaatccgca ggaatctagg caacatgcgc cactgtctac tcatctgggg
    16201 acgcccccgc acatggccta caacacgatc ctttacgacg tcgaagacgg cgttctgacg
    16261 ctgaccctaa atcggccgga acgtctgaac gcgttcaacc acgagatgct cagcgagatg
    16321 atcgacgcct tcgaccgggc agatgcagac gacgaggtga gcagcatcat cgtcacaggg
    16381 gccggtcggg cattctgtgc cggcgcggac ctcggcgccg gcggcgacac cttcaatgcg
    16441 gacggtcgcg aggaccgagc cagcggcctt cacccggacg gcggcggccg cctgaccctg
    16501 cggatctacg agctcaagaa gccgatcatc gccgcggtga acgggcccgc cgtcggcgtg
    16561 ggcgtgacca tgacgctggc catggacatc cgcctggccg cagacgtggc caagttcggc
    16621 ttcgtctttg cacgccgggg catcgtcccc gaagcctgct cgagctactt cctgccacgc
    16681 atcgtgggca tcagccaggc actggaatgg tgctacagcg gccgcgtgtt ccccgccgag
    16741 gaggctctgg ctggcgggct ggtgcgcagt ctgcactcga aggaggagct gatgccggca
    16801 gcaagagccc tcgcctgcga gatcgccgac aatacttccg cgatctcggt gacgctggtg
    16861 cgccaaatga tgtggcgaat gctgggcgcg gatcatccca tggaagccca caagatcgac
    16921 tcccgtgggg tgtacttcac cggcaagtct ccggacgctc gggagggcgt cgaagcgttc
    16981 ctggaaaaac ggcccgcccg gttcccgggc aaggtcagca gcgatctgcc ggaattcttc
    17041 ccctggtggg aggagagaac gttttcatga ggacaacgac atgagctcac cgaccattgc
    17101 ccagaaagca ccctacgcgg tagacgtgca ggccggccgc aagtatttct ggtgcgcctg
    17161 cggacgcagc gccaagcaac ccttctgtga cggttcccac gagggcacgg cgctgacccc
    17221 gctgccgtac acggcggaaa aggacgctac gctctacttc tgcggttgca agcacaccaa
    17281 gaacgttccc ctctgcgacg gctcccacaa cgacttgtga tcgtgaagga tcgccatgct
    17341 gtcgcgaatc ttcggaaaca aggaccctct gagccagccg gatccggcgg cccggcggcg
    17401 cgcggtcgag gggctgtcgg aaggtaaagc gggcgagctg caggagcggt tgctcgagct
    17461 tgcgcgcagc gacgcagatc ctggcgttcg ccaggcctgc gtgagccggc tgcagggaac
    17521 atcggacctc gagcagctgc tcgacgacgg gctgatggcg aaccgtgcag ctgagcggat
    17581 cgccgagctg ctcggccccg acgcctccag cgagctgacc agacacccgc tcgtccttcg
    17641 cgcccgcctg ttccgcctgg cgcccgagga cgcagcccgc ctgatcgatt ccatagagga
    17701 cgaggacacg ctggtggatc tgacggccgg ctcgcgggag ccgctgcgcg gaaggattct
    17761 ggcgggaatc cgatcggcca gggcgctggc ggctctggag cagcagagcc gcggtcggga
    17821 caaggggctc aatcgcttcg cccgggaagg catggagcgc atcaagcagc tcaggcgcga
    17881 ggcgcaggcc acgctggacc gcctgaacga gcttgccgac gccctcgagc gcgcatcgcg
    17941 gcaggcgccc ggcatcgccg cacgacaacg ctacgagtca ctgcttcggg agttcgatca
    18001 ggcttacgag cgttttgccg ggcacgcccg ggcgctggag ggttgcaacg aaccgcctcg
    18061 ggacgcgtca cccatccgag cccgcgtggc cgctctcgtc gcccccgcgg aaccggtggc
    18121 gatcagccgg caggaggaaa cacccgcgag cggtggggag gcgtttgccg acctcgtgcc
    18181 gcagctggaa gcgctggagg ggctgatgga agcggggcac gacttccaga cggtatccga
    18241 gacccggtcc cgtctgaccg aagcctggct gcgcgccgcc gaccacgcgc tgccgggcga
    18301 tgcagagcat cgggtgttcg agcgcgtctc ccacgcctat cagaacctgg ccgatgccgc
    18361 cgggcggctg acggcggccg gctggcagac gcatcgtgat cccctgccca gtgacctgcc
    18421 cgatgatccg ggggaagcag ccggcttctg gcaggcggcg gaggagcggc gcaggttgct
    18481 caagcgcgcg gaggggctcg ttcgagccgt tgcctggccg gattgggcga gccccacggc
    18541 tgagcttcga gacctgctcg acgacgtgga acgactgcgg gaggaggtgg cgcggtcaga
    18601 cgccctcggc agcagtcggc acgaggacct caagatgctg atcagcaccg cggcgagcgc
    18661 catcgatgag ggcgccgttc gagcggccac ggaggccctc ggccgcgccc gggcaattct
    18721 gcaggccctt cccgcgcgtg ggagggaagc ccaggccaga gcccttcagc aggagtcggc
    18781 caggctgtcc gagctgcgtg actggcagac tttcgcgacc acgccgaagc gggaggccct
    18841 ttgcgaagcc gttcagaacc tcgccgatca gcccctggat ccgccggatc aggccgagcg
    18901 aatcaaagcg ctgcgcggcg agtggaacgg tctcgggccc gtgtccggac gcgaagatcg
    18961 cgccctcgcc gatcgtttca accgcctcgc cgagcaggca ttcgagccgt gccgagccta
    19021 cttcgccgag caggccagcg tacgcgcaag caacctggtc gagcgcggga agatctgcga
    19081 tcagctggaa aactacctgg aaagcaccga ctggaaagcg gcggacatca aggcggccga
    19141 gagaattctg cgcgccgccc gggatgagtg gcgcagattc caacccgtcg accggaaccc
    19201 gggcaaaccc gtcgaggagc ggttcgaggt gctccaggaa cggctccaca agctcatcaa
    19261 agccgagtgg gatgccaacc tgcagctgaa acggcagatc gtcgaggagg cggaagcact
    19321 tgccggcagc gaagccgacc cccacgaccg ggcccaggag gcaaaagcgc tgcaacggcg
    19381 ctggcgggag ataggcatca cgcccagagg ccccgaccga aagctgtggc agcagtttcg
    19441 cggcgcgtgt gatgcgatct tcgatgcgct gggagccgcc cgggaggcat cccaccaggc
    19501 ggtcagcgag gccctcgacg ctgctgataa cctgctgacc tcctttgaga gcgaccttgg
    19561 gaatacgtca gcaaccgagg ccgaggcaga gatcctcacc ggcttccgac gccgcttcag
    19621 cgagctgccg ccactgccgg agcgggaggg gcggcagatt tcccggcgct tcgaggagtt
    19681 gacccgcagc taccgatccc ttctgcatca gaagtcccgc taccaggagc gggccgagct
    19741 cgatcgcctc agagccctgg acgaggcggt gacccacacg gagatcgcgc cgggcctgac
    19801 ggcggagccg acgcaagatg ctatcgcgga tcccgtcgtc tccgagcgtc tgcagaaaag
    19861 cggcgaaccg atccccatgg acgcccttcg cgcgctcacc gtgcgggcgg agatcctcgc
    19921 cggcgcggaa tccccggccc aggaccggga gattcgcttg aagcttcagg tcgaggagat
    19981 gaacgtcggg atgggcagcc agcggcgtgc tcaagaccct ctggagatgg cccgcgagtg
    20041 gtgcgcccgc ggtcccaaag acgaggcgtg ctcggcgctg cgcgagcggt tcttcgacgc
    20101 gctcgcacgg gcgctggagg actaggcggg caggtccagg cagatgcggg cgccccccag
    20161 ggcgctggtc tcgatacgca actctccctg atagagcgcc acgagctccg cgacgacgga
    20221 caggccgatc ccctgtccgg ggcgcatctc gtcagcgcgg ctgccacgat tgagcacctc
    20281 ctgacgcagc tccggggcga taccgggccc gtcgtcttcc acccacaccg acaccaccga
    20341 gttgcgctct gcgccgatgc gtatccgcgc cgtggtgtat ttgaaggcgt tctccagcac
    20401 gttgccgagc atctccatca gatcacgctc atcgccccga accacgaggt tctcgggaag
    20461 cgcttcggca acctcgattc cccggtcggc ataggcggtc ttcagcgcgc gcaccagccg
    20521 cgacacgacg gtggcgagat tcacagagcg gccgaccacc accggcccgg tgacggcggc
    20581 ccgggagagc tgatgggtca ccgagctctc catccggtca agctgatcgc tcagcaggga
    20641 aggcgtcgac gaatccggct ccgccagggc attgcggatg acggcgagcg gcgtcttcag
    20701 gctgtgcgcg agatcctcca tggccttgcg atagcgagag cggctgcgct gctcgtgcgc
    20761 gacgaagcgg tcgaggttct cggcaagccc ctgcagctcg ctggggaagc cgcgggaaag
    20821 cctctcccga tgcccctcct ccatctctcg cacttcctcg gccatggttc gcaacgggat
    20881 cagcccccag cggacggcaa agaactgcgc caccacgaac agcacggtga ccgcacccag
    20941 gccgatgtag aggttgcgcc ggaacccctg gacggccgcc tgaaacggtg cctgatcggt
    21001 gccgacgcgg aaggtcagca gcgcttcgtc cgcgtcctcc cagatgaccg cgtagctcaa
    21061 gaagaaacgg tcaacgagcc cctgacgggc cgactcgaaa cggaactcgc cggcgctcat
    21121 cgggacggct tcctggggga agcggacgcc cgtggtcgtc accgaaggcg accgccagcg
    21181 gtcgccgccg tcaccccggt ccaccacggc gtaaagacca ctgcctggct ggttcaggcg
    21241 cggctccggc agatccgggt tgagacggat gtcgtcgtta ccctccacca ccgcgcccat
    21301 cagggagtag atgatcaggc gcagctgctc ttcggcacct gctctgacgc tggcctgaaa
    21361 ggagcggtcg agcacgacgc ccgcgagcgc gagcgaagtg ccgagcacc
//
LOCUS       DBNHKV010000335        11420 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1536598, whole genome shotgun sequence.
ACCESSION   DBNHKV010000335 DBNHKV010000000
VERSION     DBNHKV010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11420
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1536598"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="AC16PP_10610"
     CDS             <1..693
                     /locus_tag="AC16PP_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482121.1"
                     /translation="DVAKASMSAEGLAIEATEGDRAKGNTLSFVDCGAALPSYDVSIR
                     DESGRELDERECGRIWVRGPSLMRGYFRDPVTTAEVMKPGGWLDTGDIGYRIGRRLFL
                     TSRAKDVIIINGRNIWPQDLEQLAERLPEVRLGAISAFSVTRPNQEELAVMVVESRNA
                     GRELSDELSGMVREHFGINCYIELAPPRTLPRTSSGKLSRARARADFLDRTAWDADGY
                     PMTAPEVQAQAG"
     gene            711..1643
                     /locus_tag="AC16PP_10615"
     CDS             711..1643
                     /locus_tag="AC16PP_10615"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6482122.1"
                     /translation="MPDNPTRPLIALTGATGFIGRHLQRALLDAGCAVRALVRTRSLA
                     SSGLIEGVEVAEADLGCESSMKLALNDVDQLVYAAGSVRGRDPEDFALANVTGVKLAA
                     TALASRKPGIPMLLISSLAASAPELSHYAASKRAGEDALRGSGLTRWAIMRPPAVYGP
                     GDRELKGTFTAIRRGLVPMVAPADQHLPFIEVGDLADAVNAWSQHPQACSGGTFALHD
                     GQQGGYDWQAIRQQIGPTRTVTLKVPYPLLDALSHVNLWLSRIMSYSPMLTPGKVREL
                     TYMGWTCSNDAFTDATGWVPRTRLGQGVAAMFAR"
     gene            1666..1917
                     /locus_tag="AC16PP_10620"
     CDS             1666..1917
                     /locus_tag="AC16PP_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR6482123.1"
                     /translation="MSASQTDIIVTLAELLCAQLPEPVAVAPDTDLISDLALESVQIM
                     EFIVEVEDHYDIAISLEALAEIRNLDQLARLVITALDAQ"
     gene            1914..3101
                     /gene="spt"
                     /locus_tag="AC16PP_10625"
     CDS             1914..3101
                     /gene="spt"
                     /locus_tag="AC16PP_10625"
                     /EC_number="2.3.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020797731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004758 - serine C-palmitoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046513 - ceramide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine palmitoyltransferase"
                     /protein_id="HIR6482124.1"
                     /translation="MSLLEKFDALETTRQELLGHGTNPTDVIIERVISATESVIGGKP
                     TIMAGTNNYLGLTFDPECIAAGKRALDESGTGTTGSRMANGNYASHGELEREIADFYG
                     YPYAMVFSTGYAANIGTMAALVGPGDAVMLDGDAHASLFDGSKLSGATVYRFKHNDAE
                     SLDKRLRRLGDVASRTLVIVEGLYSILGDRAPLKELAEVTRAHDACLFVDEAHSLGVL
                     GETGRGRVEEDQVEADFVVGTFSKSLGSTGGFCVSPHRALTLFRYASRPYMFTASPCP
                     SVIATTRAALAQMRKRPELRASLWRNAETLYSGLAQLGFTLGPEPSPVVAVRMTSREQ
                     ALANWQQLLDNGIYINLILPPAAPDGTALLRCSVSAAHTPEQIQRIIDAFRQLPPDPG
                     QPA"
     gene            complement(3064..4377)
                     /locus_tag="AC16PP_10630"
     CDS             complement(3064..4377)
                     /locus_tag="AC16PP_10630"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR6482125.1"
                     /translation="MNESVSDSPVRRALKNFSVLMGGRAVAGVLSVVSLTLVARFLGP
                     VEFGLLVVVHTTAVVVRGLLNFKPSDTVVRYGVAPFDDDDHRELNHLLRFTLALDVAT
                     ASLAALCMMLVMLLAGPALGLPEELAMPAVLYGLVLLASGSGTARGVLRVAGRFHAMG
                     VQQNVGPLIRLAGVAAVYLAEGGLLGYLMVWAVAFAAEHLFMNVRGWLELKRRGVRPG
                     RPTLAGAKRRFPGLWGFVGTLYWQSNLDLAQRHGLILLAGVLLGPAGAGMFRVAREFA
                     DVLAKPVIVVRQAVFPDFARLWRERDPRFKTLYWRLGLYGGLIAAVVVGLVGLYAGEL
                     LEALVGEEYVHGAALLVWLMVAGALELIGAALRPAAYAMGAATGALRVQIVTAVCHVS
                     AFAVLVPWLGLAGAGIAAACSGAIALAGMVALVLTRADQDPGAAA"
     gene            complement(4427..5344)
                     /locus_tag="AC16PP_10635"
     CDS             complement(4427..5344)
                     /locus_tag="AC16PP_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482126.1"
                     /translation="MRDARSVIVERTAPAGLENDQGVERMLSELRARCGEHLQAVLLY
                     GSYLRGVRDTLLDFYVVVDGYRGALGSPVSALGARVLPPNVYYLAVGEGAERVRAKYA
                     VVTMRQLRRQAASGLHPYFWARFAQPARIVYARWPADEAALVQIIEQCVRTFVKRVAP
                     TLRGDFTSREFWARGFALTYRSELRSESAGRSDSIIDHDPDYYDNMLAAVADGRHLVC
                     AGGRYRAVRPRVSAAVEWACVIALGKFLSVGRLVKGALTFDDALDYILWKVERHSGVR
                     VEASARQHRYPLIFAWPLVWRLYRRGAFR"
     gene            complement(5357..6541)
                     /locus_tag="AC16PP_10640"
     CDS             complement(5357..6541)
                     /locus_tag="AC16PP_10640"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR6482127.1"
                     /translation="MSIYAHLLGDSDIELWGLTSRERLRRQVAELPDAVLVDQLGTVP
                     EDATVICIRADYLFESRTIKALAERGRAVLVCPNDGGAAALVSTGREAAQLVPLLEGA
                     TTDHDLPTLAPADLAAYEDRLRKAEEPLLLPIAEEDRGELEDRLYGRSYKGITDLVTK
                     WLWPRPARHAVRVCARLGITPNMVTLAGLLLVVYAGFAFYQGDYWLGLAAGWFMTFLD
                     TVDGKLARVTVSSSRIGHVLDHGMDIVHPPFWYVLWGMSLDDSGFSVPLLEYYWWIVA
                     GYVGGRLIEAAFHGLGSCSLFDWRPFDAYFRLVTARRNPCLILMLVAMLLGSPGGAFL
                     AVVFWTVGTTVVLMIRLLQATLARMSDGPLSSWLTEPSAATAYPRAFRTFSRTRGAYV
                     QR"
     gene            6663..7574
                     /locus_tag="AC16PP_10645"
     CDS             6663..7574
                     /locus_tag="AC16PP_10645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6482128.1"
                     /translation="MRLIHLSDPHLSSLHGVKLRDLLGKRTLGYQSWYRKRRHQYTMD
                     TLRRVTRAVRNEAADAIVVTGDLVHLGMAQELRQAREWLESVGGPDRVLVIPGNHDCY
                     HRQSWALVKETIGPYLALEKDTADARDGYPLVRRSGEASVIAAGTAEPTAWWSASGSL
                     GAAQTTRLEDALIKARETFTFLAIHHPPLPGMAPRRKSLRDAHALAELLERHAPDVVL
                     HGHLHANQENSDTPTRIFCTAPPSSERPRAPASYRVFDVVEDAAGYRVVMRLKSLIGE
                     EMVVADEQSWTSGRSLDPGERDDAAKQ"
     gene            complement(7533..8600)
                     /gene="lptG"
                     /locus_tag="AC16PP_10650"
     CDS             complement(7533..8600)
                     /gene="lptG"
                     /locus_tag="AC16PP_10650"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HIR6482129.1"
                     /translation="MKRVDRYLVGVLLLGAVPVLLLLLGLFGFVTLAEELEDVGKGQY
                     EEADAIRVALLTLPRIAIDLLPVAVLLGGVMALGNLAQEQELTALRAVGLSQWRMAYP
                     LALAAIVLSYVVLLAQQFVVPRFEEQAKELQAKSLVNTMRGGETTEFWTRDGTHFIRV
                     GDVRFGMIPADVEIYDLGDRGVVQRVLQAEYADVIGPGQWLLHGVEERRILGKEVRRT
                     GRDTYEWESFLSPDQMASLIAPMETLTLGDLYRYVEYLEASGLNAHRYRFRLWQVLSS
                     PIALMAMCLLGLPFVLGSVRARPAGARVLFGATVGIGFYLVEQTSAQLALLYELPVIP
                     LAMLPDSALLLGSIVALTRVE"
     gene            complement(8597..9667)
                     /gene="lptF"
                     /locus_tag="AC16PP_10655"
     CDS             complement(8597..9667)
                     /gene="lptF"
                     /locus_tag="AC16PP_10655"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HIR6482130.1"
                     /translation="MIINRYLVRELLLPFFAVGGVLTVIFTTYSMTRFLDKAADGLLR
                     TSEVLQLTALKSFIAQEVLLPVSLFVALVLALGRLYSDWEMTALRSFGFAERQVLFPI
                     MSVALGFALVVAVFSLVGRPWAYEHLYRLEVEAEATSELERIKPGRFYLYDDNARTVF
                     IEELKAEDDEIQGLFVRALDGDDLEIISAPSGTVSEYVSDKNHQVDLRDAQVYRKTAQ
                     GADFLGIFGELRIFVPVAAVQPVGYKPKSQNSFVLRESDALVDQAEYQWRLSAPVSTI
                     LLALLAVLLGRSRPRQGRFARLTVAVVIYAVYFNVLGIGRTWVEQGTAATLWWVPATF
                     AGGIVLAYVPWARWFRRRGRSA"
     gene            9757..10266
                     /locus_tag="AC16PP_10660"
     CDS             9757..10266
                     /locus_tag="AC16PP_10660"
                     /inference="COORDINATES: protein motif:HMM:NF015901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HIR6482131.1"
                     /translation="MLKRFFAAELASAIALALVQLSCSAWAGELPALDDQDTVVVTAD
                     QAWESEDGDSLSFAGNFNLNAPDFHVASDTAVIHGPLEDPNRIVAEGSPVRFWVADPD
                     SGNRTHGTGRRLEYDRQRDLVRLEGDAVLEDDRTVMRSSELEYNTATKRLVAQGSGGV
                     EIVTNPNRD"
     gene            complement(10276..10704)
                     /locus_tag="AC16PP_10665"
     CDS             complement(10276..10704)
                     /locus_tag="AC16PP_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR6482132.1"
                     /translation="MNATAEKFGYPDTLVREYGHWLVLVRPQQVSLGSLVLVCKEPVT
                     RFSEVSRAAFTELHDVIAAAEGALSSLYSYDRINYLMLMMVDPDVHYHVIPRYEAARL
                     LGTVEIADHGWPGPPALGEFHELDAAEREALLGQLREAWS"
     gene            complement(10706..>11420)
                     /locus_tag="AC16PP_10670"
     CDS             complement(10706..>11420)
                     /locus_tag="AC16PP_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HIR6482133.1"
                     /translation="LLPLTAGTPKCGVEIAGQSLLEWQLGEVAQCGIDEVVVVTGFHA
                     DIVDDLVSRCSMVPVRTLHNPFFDASDNLGTCWVARSEMTDPFVIINGDTLFEAAILQ
                     KLLASPANTPVTLVVDRKDHYDEDDMKVVVSDGRLRRVGKRLELDDVNGESIGMMAFR
                     GLGPAKFVSKVESLMRSDMGLKLWYLSAVDQLAQSGEVGVCDIHGLSWCEVDDRDDLD
                     NAAGVVTAWRKPASRAAAD"
BASE COUNT         2193 a         3914 c         3580 g         1733 t
ORIGIN      
        1 gacgtggcca aggcctcaat gtctgcggaa ggcctggcca tcgaggccac cgagggcgac
       61 cgtgcgaagg gcaacaccct ctcgttcgtc gactgcggcg cggcgcttcc aagctacgac
      121 gtctccatcc gcgacgagtc gggccgagag ctcgacgagc gcgagtgcgg acgcatctgg
      181 gttcgggggc caagcctgat gaggggctac ttccgcgatc ccgtgaccac cgccgaggtc
      241 atgaagcccg gcggatggct cgataccggc gacatcggtt accgcatcgg caggcgtctg
      301 ttccttacct cgcgggccaa ggacgtgatc atcatcaatg gccgcaacat ctggccgcag
      361 gatctcgagc agctcgccga gcgcctgccc gaggttcggc tcggggcgat ttccgcgttc
      421 tcggttaccc gcccgaatca ggaagagctg gccgtgatgg tggtggagtc ccgcaacgcc
      481 ggtcgggagc tcagcgacga gctttcgggc atggtgcggg agcacttcgg catcaactgt
      541 tacatcgagc tggcaccgcc gaggacgcta ccgcgcacct cgtcgggcaa gctgtcccgc
      601 gcacgcgcca gggccgactt cctggatcgc acggcctggg atgccgacgg ttatcccatg
      661 acggcgccgg aagtccaggc acaagcgggc tgactcaccc agggtgatct ttgcctgata
      721 accctacccg cccgctcatc gcgctgacgg gcgccaccgg cttcatcggc cggcatctgc
      781 agcgcgcgct gcttgatgcg ggctgcgccg tgcgagcgct ggttcgaacc cgctcgcttg
      841 cgagctcggg gctcatcgaa ggcgtcgaag tcgcagaagc cgacctcggc tgcgaatctt
      901 ccatgaagct ggccctgaac gacgtcgacc agctggttta cgcggccgga tcggttcgcg
      961 ggcgggatcc cgaggacttc gcgctcgcca acgtcaccgg cgtcaagctg gccgccacgg
     1021 cgcttgcctc tcgcaagccg ggcatcccca tgctgctcat ttcctcgctg gcggcgagcg
     1081 cccccgagct gtcgcactac gcggccagca agcgcgcggg agaagacgct ctgcgaggaa
     1141 gcgggctcac caggtgggcc atcatgcggc ccccagccgt ctacggaccc ggcgacagag
     1201 agctcaaggg cacgttcacc gcaatccggc gcggcctggt gcccatggtc gctccggcag
     1261 accagcatct gcctttcatc gaggtcggcg atctggccga tgccgtgaac gcctggagcc
     1321 aacatccgca agcctgctca ggcgggacct ttgccctgca cgacggccag cagggcggct
     1381 acgactggca ggcgatccgc cagcagatcg gtccgacccg cacggttacg ctgaaagtgc
     1441 cgtacccgtt gctcgacgcg ctgagccacg taaacttgtg gctttcgcgc atcatgagct
     1501 actcgcccat gctcacgccg ggcaaagtgc gcgaactgac atacatgggc tggacgtgca
     1561 gcaacgacgc tttcaccgac gccacgggat gggttccgcg aacccgcctg ggccagggcg
     1621 tcgctgccat gttcgcccgt tgaacgaccc tgagaggtat tgcacttgtc cgctagccag
     1681 accgacatca tcgtcacgct cgcagaactg ctctgcgccc agcttccgga gccggtagcc
     1741 gtcgcccccg atacggatct catctccgat ctggctctcg aatcggtcca gatcatggag
     1801 ttcatcgtcg aggtggagga tcactacgac atcgccatca gcctcgaagc gctggccgag
     1861 atccgcaacc tcgatcagct ggcgcgtctg gtcatcaccg ccctggacgc ccaatgagcc
     1921 tcctggagaa gttcgatgcc ctggagacta cccgccagga gctcctcggc cacgggacca
     1981 acccgaccga cgtgatcatc gagagggtca tctcggcaac ggaatccgtc atcggcggca
     2041 agcccacgat catggcgggg acgaacaact acctcggcct gactttcgac ccggaatgca
     2101 tcgccgccgg caagcgggcg ctggatgaat cgggcaccgg caccacggga tcccgcatgg
     2161 ccaacggcaa ttacgcttcc catggggagc tggagcggga gatcgccgac ttctacggct
     2221 acccctacgc catggtgttc tcgaccggct acgcggcaaa catcgggacc atggcagctc
     2281 tggtgggtcc gggagacgcc gtgatgctcg atggcgacgc ccacgccagc ctgttcgacg
     2341 gcagcaaact ttccggcgca accgtttacc ggttcaagca caacgatgcc gagagcctgg
     2401 acaaacgcct gcgccggcta ggtgacgtcg ccagccgcac gctggtcatc gtcgaggggc
     2461 tctacagcat tctgggagac cgcgctccgc tcaaagagct ggcggaggtg acccgcgccc
     2521 acgatgcctg cctgttcgtc gacgaggctc actcgcttgg cgtgctcgga gaaaccggac
     2581 gcggacgggt ggaggaagac caggtggaag ccgacttcgt ggtcggcacc ttcagcaaga
     2641 gcctgggttc cacgggcggc ttctgcgtga gcccccaccg agcgctgacc ctgtttcgct
     2701 atgccagccg cccctacatg ttcacggcgt ccccatgccc gtcagtcatc gccaccacgc
     2761 gggcggccct tgctcagatg cgcaaacggc ccgagctgcg cgcgtcgttg tggaggaacg
     2821 ctgaaacgct ctacagcggc ctggcccagc tgggattcac cctcggcccc gagccgagcc
     2881 ccgtggtcgc cgttcgcatg acctcccggg aacaggcact ggcgaactgg cagcagctgc
     2941 tggacaacgg tatctacata aacctcatcc tgcccccggc cgcgccggac ggcaccgcgc
     3001 tgcttcgctg cagcgtcagt gcagcccaca cgccggaaca gattcaacgc atcatcgacg
     3061 cgttcaggca gctgcccccg gatcctggtc agcccgcgta agcaccagcg cgaccatccc
     3121 tgccagcgca atcgctcccg agcacgcagc ggcgatgccg gcacccgcca gccccagcca
     3181 gggcacgagg acggcaaaag cagaaacgtg gcatacggcg gtgacgatct ggacccgcag
     3241 cgcgccggtc gccgcgccca tggcgtaagc cgccggcctg agagccgcac cgatcagctc
     3301 caacgcacca gcgaccatca gccacacgag cagcgccgcg ccgtgcacgt attcctcgcc
     3361 tacgagcgct tcgagaagct cgccggcata aaggcccacc aggccgacga ccacggcggc
     3421 gatcagcccg ccgtaaaggc cgaggcgcca gtagagcgtc ttgaaccgcg ggtcgcgctc
     3481 ccgccacaga cgggcgaagt ccgggaacac ggcctgacgc accacgatca cgggcttggc
     3541 cagcacatcg gcaaactcgc gggcgacgcg aaacatcccg gcacccgcgg ggccaagcag
     3601 aacgcctgcc agcaggatca ggccgtgccg ctgcgcaaga tcgaggttcg actgccagta
     3661 gagcgtgccg acgaagcccc acaaccctgg aaatcgtcgt ttcgcgccgg caagggtggg
     3721 acgtccgggc cggactcctc gccttttgag ctccagccag ccacgcacgt tcatgaagag
     3781 atgctcggcg gcaaacgcga cggcccaaac catcaggtaa ccaagcaagc ctccctcagc
     3841 caggtacacg gcggctacgc cggccagccg gatgagcggc cccacgttct gctggacgcc
     3901 catggcatga aatcgccctg ctacccggag cacgcctcgc gcggtccccg agcccgatgc
     3961 cagcagaacc aggccgtaga gaacggccgg cattgccagc tcctccggga gccccagggc
     4021 ggggcccgcg agcagcatga cgagcatcat gcacagcgcg gcaaggctgg cagtggcgac
     4081 atccagggca agagtgaagc gcagcaggtg gttcagctcc cgatggtcgt cgtcgtcaaa
     4141 cggcgcgacg ccgtatcgga ccaccgtgtc agacggtttg aagttgagca ggccgcgcac
     4201 caccacggcc gtggtgtgca cgaccacgag gagcccgaac tctaccggtc cgagaaagcg
     4261 ggcaaccagg gtcagcgaaa ccacggacag cacaccggcc acggcacggc cacccatcag
     4321 cacggagaag ttcttcagcg ccctgcgaac gggcgagtcg gagaccgact cgttcacgcg
     4381 cgagctccgc cccgcgcccg cacggcggac ccggcagtgt gaccgttcac cggaacgcgc
     4441 ctctgcgata gagccgccag accagcggcc aggcaaagat cagcgggtag cgatgctgcc
     4501 gggcgcttgc ttccacccgc accccgctgt gccgctccac cttccagagt atgtaatcca
     4561 acgcgtcgtc gaaggtgagc gcgcctttga caagccggcc aaccgataga aactttccca
     4621 gcgcgatcac gcacgcccat tccacggcgg ctgaaacccg cggcctgacg gcgcggtagc
     4681 ggcctcccgc gcagaccagg tgccggccgt cagccacggc ggccagcatg ttgtcgtagt
     4741 agtcgggatc gtgatcgatg atcgagtcac ttcgcccagc cgactcggac cgaagctcgg
     4801 aacggtaggt gagcgcaaag ccccgggccc agaattcccg gctcgtgaaa tcccctctga
     4861 gcgtgggagc gacgcgtttg acaaacgtac gcacacattg ctcgataatc tgaacgagtg
     4921 ccgcctcatc ggcgggccag cgtgcataga cgatgcgggc cggctgggca aagcgcgccc
     4981 agaagtaggg atgcagcccc gaggcggcct gcctgcgcag ctgtctcatg gtcacgacgg
     5041 cgtactttgc ccgaacacgc tcggcgccct cacccaccgc aaggtagtag acgttgggcg
     5101 gcaggacccg cgcgcccagg gccgaaaccg gcgagcccag ggcgccgcgg tagccatcga
     5161 cgaccacgta gaaatccagc agggtatcgc gcacgccgcg cagataggat ccgtagagca
     5221 gcacggcctg caggtgctcg ccacaccggg cacgcagttc cgagagcatt cgttcgacac
     5281 cttgatcgtt ctcgagaccg gcaggtgccg tgcgctcgac gatgactgac ctggcgtcac
     5341 gcatcaggcg aggtgactag cgctgcacat aggctccgcg ggtccgcgaa aatgtccgga
     5401 acgcgcgggg gtatgcggtc gccgccgacg gctcggtgag ccaagaggac agcggcccgt
     5461 cgctcatgcg ggcaagcgtc gcctgcagaa gcctgatcat cagcacgacc gtggtgccca
     5521 ccgtccagaa caccaccgcc aggaacgcgc cgccaggact gcccagcagc atcgccacga
     5581 gcatcaggat gaggcaggga ttgcgcctgg ccgtgaccag tcggaagtag gcgtcgaacg
     5641 gcctccagtc gaacaggctg caggagccga ggccgtgaaa ggcggcttcg atgagccgtc
     5701 cgccgacgta gccggcaacg atccaccagt agtactccag cagggggacg ctgaaaccgc
     5761 tatcgtccag cgacatgccc cacagcacgt accagaacgg cggatgcacg atgtccatgc
     5821 cgtgatcgag gacgtgcccg atgcggctcg agctcacggt gacccgggca agcttgccgt
     5881 cgaccgtatc gagaaaagtc atgaaccatc cggccgccag acccagccag tagtcgccct
     5941 ggtagaaggc aaaccccgca taaacgacga gcaggagacc cgccagcgtg accatgttgg
     6001 gcgtgattcc caaccgcgcg cagacccgga ccgcgtgacg cgcgggtctg ggccacagcc
     6061 acttcgtcac caggtcggtg atccctttgt aggaacggcc atacagccgg tcctcgagct
     6121 cgccacggtc ttcttccgcg atcggcagca gaagcggctc ttcggccttt cgcaggcgat
     6181 cctcatacgc ggccaggtca gccggcgcga gcgtcggtag atcgtgatcc gtcgtcgcgc
     6241 cttcgagcag cggaaccagc tgcgcggctt cccggcccgt gctcaccagg gcggccgccc
     6301 cgccgtcgtt ggggcaaacc agaaccgcac gcccgcgctc ggccagggct ttgatcgttc
     6361 tcgactcgaa gaggtaatcc gcgcgaatgc agatcaccgt agcgtcctcc ggcacggtgc
     6421 cgagctgatc caccaggacc gcgtcgggaa gctcggcgac ctgtcggcgc aggcgctcac
     6481 gtgaggtcag tccccaaagc tcaatatcgg agtcgccgag caggtgtgcg taaatgctca
     6541 tgttatagtt ggaaattcac tgcgcacttg gggatcctgg gacttgcggc gggcgtttac
     6601 aggccaagcc gccgacggtc gcggattata ggcatgcccc agttgctcgg caaagggcgg
     6661 ccatgcgtct aatccatctc agcgatccgc atctttcatc cctgcacggc gtgaagcttc
     6721 gcgacctcct cggcaagcgg acgctcgggt accagtcctg gtaccggaag cggcgccatc
     6781 agtacaccat ggacacgctt cgcagggtga cgcgcgcggt ccgcaacgag gccgcggacg
     6841 ccatcgtggt cactggcgac ctcgtgcatc tcggcatggc gcaggagctt cgccaggcca
     6901 gggagtggct cgagtcggtg ggcggcccgg atcgggtgct ggtcattccc ggcaaccatg
     6961 attgctacca tcgccagtcg tgggccctgg tcaaagagac gatcggcccc tacctggcac
     7021 tcgagaagga cacggcggac gcgcgcgacg gctaccccct ggtgcgacgc tccggcgagg
     7081 cgagcgtcat cgccgccggc actgccgaac ctactgcctg gtggtccgcc tcaggctcgc
     7141 tgggcgccgc ccagacaacg cgcctggagg acgcgctgat aaaggcgcgg gagacgttca
     7201 ccttcctcgc cattcaccac ccgccgctgc caggcatggc gccgcgccga aagtcgcttc
     7261 gcgacgcgca cgccctggca gagctcctcg aacggcacgc gcccgacgtg gtcctgcacg
     7321 gccatctgca tgccaatcag gaaaactccg acacgcccac acgcatattc tgcacggcgc
     7381 cgccgtccag cgagcgaccg cgggcgcccg cttcctaccg cgtgttcgac gtcgtcgaag
     7441 acgcggccgg gtatcgcgtc gtcatgcgcc tgaagtcgct gatcggcgag gagatggtgg
     7501 tcgcagacga gcagtcgtgg acctccggcc gatcactcga cccgggtgag cgcgacgatg
     7561 ctgccaagca gtagcgcgga gtccggcagc atcgccaggg gtatgaccgg aagctcgtag
     7621 agcagtgcga gctgagcgga cgtctgctcg accagataaa aaccgatgcc gaccgtggcg
     7681 ccgaacaaca ccctggcgcc cgctggccga gcccgcaccg aaccgagaac gaacggcaag
     7741 ccgagcagac acatggccat cagcgcaatg ggcgagctga gcacctgcca cagcctgaag
     7801 cggtagcgat gagcgttgag acccgacgcc tccagatatt cgacgtaccg gtagaggtcg
     7861 cccagggtca gggtttccat cggcgcgatg agggaggcca tctggtccgg agacaggaag
     7921 ctctcccact cgtaggtgtc acggccggtg cgcctgacct ctttgccgag aattcggcgc
     7981 tcttcgacac cgtgaagcag ccactggccc ggcccgatca cgtccgcgta ctcggcctgc
     8041 agcacgcgct gcacgacgcc gcgatcgccg agatcgtaga tctcgacatc agcgggaatc
     8101 atgccgaaac ggacgtcccc caccctgatg aagtgagtgc cgtcgcgggt ccagaactcg
     8161 gtggtctcgc cgccacgcat cgtgttgacc agcgacttcg cctgaagctc cttggcctgt
     8221 tcctcgaacc gaggcaccac gaactgctgc gccagcagca cgacgtaaga caacacgatg
     8281 gccgccagcg caagcggata ggccatccgc cattgggata gaccaaccgc tcgcagcgcc
     8341 gtcagctcct gttcctgagc caggttgccg agcgccatca ccccaccgag cagcaccgcc
     8401 accgggagaa gatcgatggc gattcgcgga agcgtcagca gggcgacgcg gatcgcgtcc
     8461 gcttcctcgt actgcccctt gccgacgtcc tccagctctt cagcaagggt gacgaaaccg
     8521 aacagcccca gcagcagcaa cagaacgggt accgcgccca gcagcaagac gcctacaagg
     8581 tagcggtcca cacgcttcac gcgcttcgcc cccgtcgtct gaaccaccgg gcccatggca
     8641 cgtaggccag cacgatgccg cctgcaaagg tcgccgggac ccaccacaac gtcgccgccg
     8701 tgccctgctc gacccaggtc cttccgatgc ccagaacgtt gaagtacacg gcgtagatga
     8761 ccacggcaac cgtgagccgg gcaaagcgcc cctgacgggg ccgggaccgt ccgagcagga
     8821 cggccagcaa cgccagcaga atggtcgaaa cgggcgccga caggcgccat tggtactcgg
     8881 cctgatcgac tagcgcgtcg gattctctga ggacgaacga gttctgggat ttcggcttgt
     8941 agcccacggg ctgcaccgcc gccaccggaa cgaatatgcg cagctcgccg aagatgccca
     9001 gaaagtcggc gccctgcgca gttttcctgt agacctgagc atcgcgaagg tcaacctggt
     9061 ggttcttgtc gctgacatac tcgctcacgg taccgctggg cgcggagatg atttccagat
     9121 catcgccgtc cagcgcgcgc acgaacagcc cctgaatctc gtcgtcttcg gccttcagct
     9181 cctcgatgaa gacggtccgg gcattgtcgt cgtacaggta gaatcgcccg ggtttgatgc
     9241 gctcgagctc cgaggtcgct tccgcttcga cctccagcct gtagaggtgc tcgtacgccc
     9301 agggcctgcc caccagggag aaaacggcga cgacgagcgc aaagcccagg gcgacgctca
     9361 tgatcgggaa aagcacctga cgctcggcaa acccgaacga gcgcagggcc gtcatctccc
     9421 agtcgctgta gagtcgcccg agggcaagca ccagggcgac gaagaggctg accggcagca
     9481 gcacttcctg agcgatgaaa gacttgagcg ccgtaagctg cagcacctcc gacgtccgca
     9541 gcaaaccgtc ggcagccttg tcgaggaacc gggtcatgct gtaggtggtg aatatcaccg
     9601 tcaacacgcc gccaaccgcg aaaaacggca gcagaagctc gcgaacgagg taccggttga
     9661 tgatcagtgc tctaaccctc tcccgaactg caggccagcc cgtcgccgga tcctgatatc
     9721 atcgacccgg tttcccgacg accccggccg agctccatgc tgaaaagatt tttcgccgcg
     9781 gaactggctt cggccattgc gctggcgttg gtgcagctct cttgctcggc atgggccggc
     9841 gagctgcccg cgctggacga tcaagatacc gtagttgtca ccgccgacca agcttgggag
     9901 tccgaggacg gcgactcgtt gagctttgcc ggcaacttca acctcaacgc ccccgacttc
     9961 cacgtcgcat ccgacacggc ggtcattcac gggcctttgg aggatcccaa ccgcatcgtc
    10021 gcggagggca gccccgtgcg cttctgggtt gccgatcccg attccgggaa caggactcac
    10081 ggaaccggca ggcggctgga atacgatcgg caaagggatc tggtccggct cgagggggat
    10141 gccgtactgg aagacgaccg gaccgtgatg cgaagttccg agctcgaata caacaccgcc
    10201 acgaaacgac tcgtcgcgca aggatcgggc ggcgtggaga tcgttacgaa cccgaaccgc
    10261 gactgagcac ccggctcagc tccaggcctc acgcaactgc ccgagcaggg cttcacgttc
    10321 ggccgcgtcg agctcatgaa actcccccag ggccggcggt ccgggccagc cgtggtcggc
    10381 tatctccacc gtccccagaa gacgggcggc ctcgtagcgg ggaatgacgt gatagtgcac
    10441 gtcggggtcg accatcatca gcatcaggta gttgatcctg tcgtagctgt acaggctcga
    10501 cagggcccct tcggccgcgg ctatcacgtc gtgaagctcg gtgaacgccg ccctgctgac
    10561 ctcggagaat cgggtgacag gctctttgca gaccaggacc agggagccga gagagacctg
    10621 ctgcggccgg acgagaacca gccagtgacc gtactcgcgc accagcgtgt ccggatagcc
    10681 gaatttctct gccgtggcgt tcaagtcaat ccgccgccgc ccgcgaggcg ggcttgcgcc
    10741 aggcagtgac cacgcccgcc gcgttgtcga gatcgtcccg gtcgtcgact tcgcaccatg
    10801 acagaccgtg aatgtcgcaa accccaacct cgccgctctg agcgagctga tcaacggcag
    10861 agaggtacca gagcttcaaa cccatgtccg aacgcatcag cgactccacc ttgctcacga
    10921 acttggcagg acccagaccc ctgaatgcca tcatgccgat ggactcaccg ttcacgtcgt
    10981 ccagctcgag tcgcttgccc acacgccgaa gacgcccgtc gctgaccacg actttcatgt
    11041 cgtcctcgtc gtagtgatcc ttacggtcca ccacgagcgt aacgggcgta ttcgcgggag
    11101 atgccagcag cttctgcagg atggccgcct cgaacagcgt gtcgccgttg atgatgacga
    11161 aggggtcggt catttcgctg cgcgcgaccc agcaggtgcc aaggttgtcg ctcgcatcga
    11221 agaaggggtt gtgcagggtt ctcaccggga ccatgctgca gcggctgacc aggtcatcga
    11281 cgatgtcggc atggaaaccg gtaacgacca cgacctcgtc gatgccgcat tgagccacct
    11341 cgccgagctg ccattccagc aggctctggc cggcgatctc gacgccgcat ttgggcgtcc
    11401 cggccgtcag cggcagcagc
//
LOCUS       DBNHKV010000336         8640 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_614340, whole genome shotgun sequence.
ACCESSION   DBNHKV010000336 DBNHKV010000000
VERSION     DBNHKV010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8640
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_614340"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1214)
                     /locus_tag="AC16PP_10675"
     CDS             complement(24..1214)
                     /locus_tag="AC16PP_10675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6482134.1"
                     /translation="MTNDVYLAAGTRSALGNFGGSLKDVPMTELASHVARACIERSGA
                     NPDSVDHMVFTTTCPTDRDSLFSARVVGVKSGIPEMASALDVSRACASGLQAILSAGQ
                     QIESGQSRLALAGGSEMFSRVPYAVTTARWGIVRGNQQLEDMLEWCYRCPFSLEYMGE
                     TAENLTEEFQYDREAMDEYAVSSQARALAAIESGFLARQIVPVDVPDGRGTRTFEVDE
                     SPREGIDMERLARLKPAFREGGKVTAGNSSGVTDGAAAILVGSRGALESESVEPEARV
                     VDWAVVGVPPRIMGHGPVPAIQLLLERTGLSISDIDYFEVNEAFAVVNLHVERHLGIP
                     RDAHNLYGGGISVGHPPGVTGLRMAINAIQHLRETGGRYAILSMCMGGGQGMAMLIEN
                     AARH"
     gene            1343..2212
                     /locus_tag="AC16PP_10680"
     CDS             1343..2212
                     /locus_tag="AC16PP_10680"
                     /EC_number="1.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR6482135.1"
                     /translation="MTVIGVVKEIWRFPVKSMQGSTIETCTISRTGIPGDRGWAMRDE
                     ERQEIQWGKRHPELMLCSASYRGEPEPGQTTPVDVTFPDGETLGSDDPGIHEKLSELV
                     GRRASLWPVQPPEDEAFYRRYKPDEEQFAREIAEVFAREPGEPMPDLSQFPPEILDYV
                     SVPGTFFDNEELNLVTTASIDHLRARNPAANWDIRRFRPNFYVQTPDDMTGLAENEWV
                     GKTIRVGTAVMRITAETPRCGMTVRPQAEIEFDKSILRTIVRESHQNLGVGAHCIEDG
                     RISVGDALVIVDA"
     gene            2824..4878
                     /locus_tag="AC16PP_10685"
     CDS             2824..4878
                     /locus_tag="AC16PP_10685"
                     /inference="COORDINATES: protein motif:HMM:NF014869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HIR6482136.1"
                     /translation="MAREPLGRTLAMLPLLVAMTATAADRQDLRTNAHAISTLHKQTD
                     QGGSPNYHAAFGLGQQEGLTAVSATTDANNVTHTRYRQTLRGVPVWGEQIIISREASG
                     AVRGARGGLIEGLSAHQVPAIPTVAGAAALSATKNRIAAGMSQNPVFERESAELVIYL
                     HDGTPRLSWAVSFFADVPGGGEPTRPTVLLDATTGEVIFEYEGLTHAEGTGPGGNAKV
                     GGYHYGSSEPAMDVAASGTTYTMNNANLKTVNLNHGTSGSTAYSYQGPENVHKEINGA
                     YSPLNDAHFFGGVVYDMYNAWVGTPPLTFQLTMRVHYGNNYENAFWNGSAMTFGDGAS
                     TFYPLVSLDVSAHEVSHGFTEQNSNLVYSGQSGGINEAFSDMSGEAAEFYMRGSNDWL
                     VGADIFKSPSGALRYMYDPTLDGRSIGSANDYVNGMDVHYSSGVFNKAFYLLATTGGW
                     GVQKAFQTFARANQLYWSSGETFRTAAMGVQDAASDLGFSTADVEAAFSAVDVNLGDV
                     CPAEQVAELGNGASSGEFGASAGEWRCFRLSVTAGGTDASFVLAKTAKGRGGDADLYV
                     QHAALPDTVRYDCRSVSSTSSESCAIATPLEGDWYVGVYAYSSYPGVTLTGSFTDPSG
                     SGGGGGDGGGSGSTDADSDGYTSDVDCDDNNPDVHPDHNDTKGRWGRDGVDNDCNGII
                     DG"
     gene            complement(4926..6170)
                     /locus_tag="AC16PP_10690"
     CDS             complement(4926..6170)
                     /locus_tag="AC16PP_10690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HIR6482137.1"
                     /translation="MTSAARIAGLPSYFIVFVASMVAIGPFAIDTYLPAMPAMATNLG
                     VEIVSVNATLSTYLFGFAFGQMFGGPISDQIGRRRIGLLGLVVFSAASVLIALAESIE
                     VILWLRALQAVGGGFATVICMAMVRDAYEPMEAAKRFPVVMLVMLGAPLAAPAIGAAL
                     LSFGWPSIFIFLALYGLAVLVAFLPVPETASMRTGKLELGRILPQYAEVITRRVEGRL
                     VPARYILTQGLLMSGTFVFITNSSFIYLEYFGIAEEWFVVYFGVNILMMMVFTLITTR
                     LIHRVAPDRLWRAARGLQFLAVVMLALGSWLFDLNIWTLTGLLSVVIGANGMINPSAS
                     GLYLSYFDRLSGSAVSLMNVAVFLFGSVLGVVTGVFFDGTLKPIVFTMAVAITVGNLN
                     ALGIPPPKNLAPDASGESASAG"
     gene            complement(6189..7820)
                     /locus_tag="AC16PP_10695"
     CDS             complement(6189..7820)
                     /locus_tag="AC16PP_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6482138.1"
                     /translation="MGETVAEETVAADADAVVIGAGFAGLYMLHKLRQCGFSVRGFEA
                     GDDVGGTWYWNRYPGARCDVESLDYQFAFSDELQRGWTWSERYPTQAEILRYLGYVAD
                     TLDLRRDIRFETRVSAARFDEAHNLWRVTTDRGEELSARYVIAAVGCLSVAQIPDIPG
                     LQDFEGETYHTGNWPHQPVDFTGRTVGIIGTGSSGVQAIPVIAAQAKALVVFQRSPNF
                     SVPARNAPLPADRAERYKDEFLAAREAACRTDFGNTTWRSLPSALEADPDERAAEYER
                     RWQEGGAGFMVTYSDLMSDRAANDTAAEFLRGKIRAIVRDPETAELLSPRDHPIGAKR
                     ICVDTDYYETFNRENVKLVDVRSHPIERITASGISTTAGDYDLDCLIFATGFDAMTGP
                     LTSMDIRGRDGVSLRAAWQDGPRCYLGLGVAGFPNLFTITGPGSPSVLSNMVLSIEQH
                     VEWIADCLVKLRKDGAEAIEALPDAQDGWIEHVNEAADATLFPQGNSWYIGANVPGKP
                     RVFMPYTGGVRAYREKCEEVVGNSYEGFDIRGGAS"
     gene            complement(7833..>8640)
                     /locus_tag="AC16PP_10700"
     CDS             complement(7833..>8640)
                     /locus_tag="AC16PP_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6482139.1"
                     /translation="QLPLRVIASQIGCPMEDLPLLRKWTEAFIANLSQQLDQPGRVQA
                     AKDMVECQHYFVERIEERRRAPTDDILSKLVNARFDDDKPLDNPECLSMLQQILVAGN
                     ETTSSSLTEGMWLLIRNPDQYEMIRRDPSPEMISKLVEEVLRISSPSANMFRRTTRDV
                     EVSGVTIPANSICFARFAAANQDDEQFPDPLKFDITRPNLKEHVAFGKGVHHCLGAAL
                     SRREMNGGFKVIFERMENFRLTPDTPEPVFMPNALLHGLARLDLSFDKIR"
BASE COUNT         1616 a         2857 c         2802 g         1365 t
ORIGIN      
        1 tcccaggtcc atcttcgtcg ctcctaatgc cgggccgcgt tctcgatgag catggccatg
       61 ccctggccac ctcccatgca catcgagaga atggcgtagc gcccgccggt ttccctgaga
      121 tgctgaatgg cgttgatggc catgcgcaag ccggtgaccc ccgggggatg gccaacggag
      181 atgccaccgc cgtagaggtt gtgagcatcc ctgggaattc ccaggtggcg ctccacgtgc
      241 aggttcacca cggcgaacgc ctcgttcacc tcgaaatagt cgatgtccga aatgctcagc
      301 ccggtccgtt ccagcagaag ctgaatggca ggcaccggtc catgtcccat gatccgcggc
      361 ggtacgccca ccaccgccca gtcgacgacg cgggcttccg gttccaccga ctcggactcg
      421 agcgcccctc ggctgcccac gaggatggcg gcggcaccgt ccgtgacgcc ggacgagttg
      481 ccggccgtga ccttcccgcc ctcccggaac gcgggcttca gacgtgcaag acgctccatg
      541 tcgatgccct cgcgggggga ttcgtccacc tcgaaggtgc gcgtgccgcg tccgtcggga
      601 acgtccacgg gcacgatctg cctggcgaga aagcccgact cgatggcagc cagagcccgc
      661 gcctggctgg acaccgcgta ctcatccatg gcttcccggt cgtactgaaa ttcctcggtg
      721 aggttctcgg cggtttcgcc catgtactca aggctgaacg ggcaccgata acaccactcg
      781 agcatgtcct ccaactgctg gttgccccgg acgatgcccc agcgggcggt ggtcaccgcg
      841 tacggcaccc ggctgaacat ctccgacccg cctgccaggg ccagccggct ctgccccgac
      901 tcgatctgct gacccgcgga gaggatggcc tgcagaccgg aggcacaggc gcgggagacg
      961 tccagggctg acgccatctc aggtatgccg ctcttcacgc ctaccacgcg ggcagagaag
     1021 agcgagtccc gatccgtggg gcaggtggtg gtgaagacca tgtggtcaac ggaatccggg
     1081 ttcgcgccgg aacgttcgat gcaggcgcgc gccacgtggg aggcaagctc cgtcatgggc
     1141 acgtctttga gcgaaccgcc gaaattgccc agcgccgagc gggtgccggc ggcgagatac
     1201 acgtcgtttg tcatcactcc ctcccgtcag ggcattctag gtcagcgcca gagcgcccgc
     1261 cagaacgttc ccgagatcca gattggacgc gactacccca cagtgcgacg attgcggtgc
     1321 atccaaagag gggagatctc gagtgactgt catcggcgtg gtgaaggaga tctggcgctt
     1381 cccggtcaaa tcgatgcagg ggagcacgat cgagacgtgc accatatcca ggacgggcat
     1441 tcccggcgac aggggctggg cgatgcgcga cgaggagcgc caggaaatcc agtggggcaa
     1501 gcgtcatccc gagctcatgc tgtgcagcgc ctcctatcga ggtgaacccg agcccgggca
     1561 gaccactccg gtcgacgtca cttttcccga cggcgagacc ctgggcagcg acgacccggg
     1621 cattcacgag aagctcagcg agctggttgg caggcgggcg tccctgtggc cggttcaacc
     1681 gccggaggat gaagcctttt accggcgcta caagcccgac gaggagcagt tcgcccggga
     1741 gatcgctgag gtatttgccc gcgagccggg cgagccgatg cccgacttga gccagtttcc
     1801 ccccgagatt ctggactacg tctccgtacc gggcacgttc ttcgacaacg aggagctgaa
     1861 cctcgtcacc accgcctcca tagaccatct ccgggcaagg aaccctgctg ccaactggga
     1921 cattcgccgc tttcgaccaa atttctatgt tcagacgccg gacgacatga cgggcttggc
     1981 cgagaacgag tgggtcggca agaccatcag ggtcggaacc gccgtcatga ggatcactgc
     2041 ggagacaccg cgttgcggca tgacggtgag gcctcaggcc gagattgagt tcgacaagtc
     2101 catactgcgc acgatcgtca gagagtcaca ccagaatctc ggcgtcggcg cgcactgcat
     2161 cgaggatggc cggatcagcg tcggcgacgc gctggtgatc gtggacgctt gaatcatggg
     2221 cgttctgctc gatcgcccac aacgccctcc tgctcttgga gatcttaaaa aaaacgggcc
     2281 cagccgataa gaccgctgat cggccggatg cctaaatccc taccggtggc aggcgtccgc
     2341 gccgcaagcg gcccggactg ctgctggtcg cgccgttgcc ggagaccgca cagggttggg
     2401 atcacatcgc gcctgcgtag gacctttccc gctttcaggt ctcccacatt ttcaaaggta
     2461 cggtccacga atccatcccc ctgctgagcc ggctgcgcag caccctaatc gcggacctga
     2521 tcggcctgcc ggatgaacac aacctggtgg tcgcgctggc ggaagtggcg actgccggct
     2581 cgctgatgct gcggcgctgc cgccctgctc ggatccggtc gtgaacgact tcacgccaat
     2641 caagggaagt gcgtgacgcg ggtcacagtt tgggcaggag ggccgcggcg ggcccgccga
     2701 aaagcaagga aaacattaca tctgcgcaac caaccgcccg caaaacccac cccatcgccc
     2761 aagtaccatg ctagagattc atttctcatg aaaagcaacc ccagcagtaa caaggagaac
     2821 cccatggcac gcgaacccct tggccgcacc ctggccatgc tgccgctcct ggtggccatg
     2881 accgctaccg cagccgaccg gcaggatctc agaacgaatg cccacgccat cagcaccctt
     2941 cacaagcaga ccgaccaagg gggcagcccc aactaccacg ccgctttcgg cctcgggcag
     3001 caggaaggcc tcacagccgt cagcgccacc accgacgcaa acaacgtcac ccacacccgt
     3061 taccgtcaga ccctgcgagg ggtgccggtg tggggcgagc agatcatcat cagccgggag
     3121 gcttccgggg cagtgcgcgg cgcacggggc ggcctgatcg aggggctgtc agcgcaccag
     3181 gtgcccgcaa tcccgacggt cgccggggcg gcggcgttga gcgccaccaa gaaccgcatc
     3241 gcggccggca tgtcacagaa ccccgtcttc gagcgggaga gcgccgagct ggtgatctat
     3301 cttcacgacg gcacaccccg cctgagctgg gcggtgagct tttttgccga cgtgcccggc
     3361 ggcggcgagc cgacccggcc gacggtgctc ttagacgcca cgaccggcga agtcatcttc
     3421 gagtatgagg gcctgaccca cgctgaaggc acggggcccg gcggtaatgc aaaggtgggc
     3481 ggctaccact acggttccag cgagcctgcc atggacgttg ccgccagcgg caccacctac
     3541 accatgaaca acgccaacct gaaaacggtg aacctcaacc acggaacctc gggctctacg
     3601 gcctacagct atcaaggccc ggagaacgtg cacaaggaaa tcaacggcgc ttacagcccg
     3661 ctgaacgacg cccacttctt tggcggcgtg gtctacgaca tgtacaacgc ctgggtgggc
     3721 acgccgccgc tgacctttca gctgaccatg cgcgtgcact acggcaacaa ctacgagaac
     3781 gccttctgga acggctcagc gatgaccttc ggcgacggtg ccagcacctt ctaccccctg
     3841 gtgagcctgg acgtctcggc acacgaggtc agccacggct ttaccgagca gaactcgaac
     3901 ctggtctact ccggacagtc gggcggcatc aacgaagcgt tctccgacat gtccggcgag
     3961 gcggccgagt tctacatgcg cgggtcgaat gactggctgg tcggcgcaga catcttcaag
     4021 agcccaagcg gcgccctgcg ctacatgtac gaccccaccc tggatggccg ctccatcggc
     4081 agcgccaacg actacgtcaa cggcatggac gttcactact ccagcggcgt gttcaacaag
     4141 gccttctacc tgctcgcaac cacaggcgga tggggcgtcc agaaggcgtt ccagacgttc
     4201 gcccgtgcaa accagcttta ctggtcctcc ggagaaacgt tccgcaccgc cgccatgggc
     4261 gtgcaggatg cggcctcgga tctgggcttc tccactgccg acgtcgaggc tgccttcagc
     4321 gccgtggacg taaatctggg tgacgtgtgc ccggccgagc aggtcgcaga gctgggcaac
     4381 ggcgcgagca gcggggagtt cggcgccagc gccggcgagt ggcgctgctt caggctgagc
     4441 gtcaccgcag gcgggaccga tgcctccttc gtgctggcaa agacggccaa gggtaggggc
     4501 ggcgacgccg atctctacgt ccagcacgca gcgctgccgg acaccgttcg ctacgactgc
     4561 cgctcggtga gcagcaccag cagcgagagc tgcgccatcg ccacgcctct cgaaggcgac
     4621 tggtacgtgg gcgtctacgc ctacagctcc taccccggcg tgacgctcac cggcagcttt
     4681 accgacccca gcgggtcggg cggcggtgga ggagacgggg gcggatcggg gtcgaccgac
     4741 gctgactccg acggctacac gtcggacgtg gactgcgacg acaacaaccc ggacgtgcac
     4801 cccgaccaca acgacaccaa gggccgctgg gggcgggacg gtgtcgacaa cgactgcaac
     4861 ggcatcatcg acggctgacg caggtcacac gactacgggc gcccgcgggc gcccgatttt
     4921 ttccatcagc ctgccgacgc cgactctccg cttgcgtccg gcgccagatt cttgggcggc
     4981 gggatgccca gcgcgttcag attgcccacg gtgatggcca ccgccatggt gaagacgatg
     5041 ggcttcaacg tgccgtcgaa gaacactccg gtgaccacgc cgagcacgct gccgaagagg
     5101 aagacggcca cgttcatcag cgacaccgcg ctgccgctca gccgatcgaa gtaggacagg
     5161 tagaggccgg acgcggacgg gttgatcatc ccgttggcgc cgatgacgac ggacagcaag
     5221 ccggtcagcg tccagatgtt cagatcgaac aaccacgagc ccagggcaag catcaccacg
     5281 gccagaaact gcagcccgcg cgccgcgcgc cagagacggt ctggcgcaac ccgatggatc
     5341 agccgggtag tgatcaatgt gaacaccatc atcatcagga tgttcacgcc gaaatagacg
     5401 acgaaccact cctcggcaat gccgaagtat tccaggtaga tgaacgagga gttggtgatg
     5461 aacacgaacg tcccggacat gagaaggccc tgggtgagga tgtagcgcgc cggcacaagc
     5521 ctgccctcga cccgccgggt gatcacctcg gcgtactggg gcaaaatccg gccgagctcg
     5581 agcttgcccg ttcgcatcga agcggtttcc ggcaccggca ggaaggcgac gagcacggcc
     5641 aggccgtaca gggcgaggaa gatgaagatg ctcggccagc cgaagctcag cagcgcagcg
     5701 ccgatcgccg gcgccgccag gggggcgccg agcatgacaa gcatcaccac gggaaagcgc
     5761 ttggccgctt ccatgggctc gtaggcgtcg cgcaccatgg ccatgcagat caccgtggcg
     5821 aacccgccgc caacggcctg caacgcccgc agccagagga tgacttctat ggattcggcc
     5881 aaggcgatga gcacggacgc ggcagagaag acgactaggc ccagcagccc gatgcggcgc
     5941 cggccgatct gatcggagat cgggccgccg aacatctgcc cgaaagcaaa gccgaagagg
     6001 tacgtgctga gcgtggcgtt gaccgagacg atctccacgc caaggttcgt ggccatcgcc
     6061 ggcatggcgg gcaggtaggt atcgatggca aagggcccga tcgccaccat ggacgcgacg
     6121 aacacgatga agtagctggg gagacccgcg atccgggcag cgctcgtcac gtcagttgcc
     6181 ggccgggctc agctcgcgcc gccgcgaatg tcgaagcctt cgtaactgtt gccgaccacc
     6241 tcttcgcact tctcgcgata cgcgcgcacg ccgccggtat aaggcatgaa gaccctgggt
     6301 ttgcccggga cgttggcgcc gatgtaccag gagttgccct gagggaacag cgtggcgtcg
     6361 gcggcctcgt tgacgtgctc gatccagccg tcctgggcgt cggggagagc ctcgatggcc
     6421 tccgcgccgt ccttacgcag cttcaccagg cagtcggcaa tccactccac gtgctgctcg
     6481 atggatagca ccatgttgct cagcacggaa gggctgcccg gcccggtgat ggtgaagagg
     6541 ttggggaatc ccgcaacgcc caggcccaga tagcagcgcg gcccgtcctg ccaggccgcc
     6601 ctcaggctga cgccatcgcg accgcggatg tccatggacg tgagcgggcc cgtcatggca
     6661 tcgaaaccgg tagcgaagat caggcagtcg agatcgtagt cgccggccgt cgtgctgatc
     6721 ccgctcgccg tgatgcgctc gatgggatga gaacgtacgt ccacgagctt cacgttctcg
     6781 cggttgaagg tttcgtagta gtcggtgtcc acgcagatgc gcttggcccc gatggggtgg
     6841 tcgcggggcg agagcagctc ggccgtctca gggtcgcgga cgatggcgcg gatctttcct
     6901 ctgagaaatt cggccgcggt atcgttggcc gcccggtcgg acatgaggtc gctgtaggtg
     6961 accatgaagc cggcaccgcc ttcctgccag cggcgctcgt actccgccgc gcgctcgtcc
     7021 ggatcggctt ccagggccga cggcaggctt ctccacgtgg tattgccgaa gtcggtccgg
     7081 caggccgctt cgcgcgcggc cagaaactcg tccttgtagc gctcggcccg atcggccggc
     7141 aggggagcgt tgcgggccgg cacgctgaag ttgggcgagc gctgaaatac cacgagcgcc
     7201 tttgcctggg cggcgatcac gggtatggcc tgcacgcccg aggagcccgt gccgatgatg
     7261 ccgacggtgc gcccggtgaa atccaccggc tggtggggcc agttacccgt gtgataggtc
     7321 tcgccctcga agtcctgcag cccggggatg tcggggatct gggccacgga gaggcagccc
     7381 acggcggcga tcacgtagcg ggcgctgagc tcctcgcccc ggtccgtggt cacccgccaa
     7441 aggttgtgcg cctcgtcgaa gcgcgcggcg ctcacccggg tctcgaaccg gatatcccga
     7501 cgcagatcga gggtgtccgc cacgtagccg aggtagcgca ggatctcggc ctgggtgggg
     7561 tagcgctcgg accacgtcca gccgcgctgc agctcgtcgg agaaggcgaa ctggtagtcc
     7621 aggctctcca cgtcgcagcg cgcgcccggg tagcggttcc agtaccaggt gccgcccacg
     7681 tcgtcgcctg cctcgaagcc gcgtaccgag aagccgcact gccgcagctt gtgcagcatg
     7741 tagaggccgg cgaagcccgc accgatgacg acggcatcgg cgtcagcagc gacggtctcc
     7801 tctgcaacgg tctcccccac gcccgcttgc gatcaacgga tcttgtcgaa ggaaagatcc
     7861 agccgcgcca gcccgtgcag caaggcgttc ggcatgaaca ccggctccgg cgtgtccggc
     7921 gtcaggcgga agttctccat gcgctcgaag atgaccttga acccaccgtt catctcacgg
     7981 cgcgacagcg ccgcgccgag acagtggtgc acgcccttgc cgaacgctac gtgctccttc
     8041 aggttgggcc gggtgatgtc gaacttcagc ggatccggaa actgctcgtc gtcctggttg
     8101 gccgccgcaa agcgggcgaa gcagatggag ttggcgggga tggtcacgcc gctcacttcc
     8161 acgtcccgcg tggtgcggcg gaacatgttg gccgacgggc tggagatgcg cagcacctcc
     8221 tccaccagct tgctgatcat ctccggcgac gggtcccggc ggatcatctc gtactggtcg
     8281 ggattgcgga tcagcagcca catgccttcg gtgagggacg acgaggtggt ctcgttgccg
     8341 gccaccagaa tctgctgcag catcgacagg cattccgggt tatccagcgg cttgtcgtca
     8401 tcgaagcggg cgttgacgag cttggagagg atgtcgtccg tcggcgcgcg gcggcgctcc
     8461 tcgatgcgct ccacgaagta gtgctggcac tccaccatgt ccttcgccgc ctgcacccgg
     8521 cccggctggt cgagctgctg ggagagattc gcgatgaagg cttccgtcca cttcctgagc
     8581 agcggcaggt cctccatagg gcagccgatc tgggaggcga tgacccgcag cggcagctgc
//
LOCUS       DBNHKV010000337         3200 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_756661, whole genome shotgun sequence.
ACCESSION   DBNHKV010000337 DBNHKV010000000
VERSION     DBNHKV010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_756661"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..910)
                     /locus_tag="AC16PP_10705"
     CDS             complement(53..910)
                     /locus_tag="AC16PP_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6482140.1"
                     /translation="MPEYDRSAEDVGNVQMLEHVNVTVPDQGLAALFYVTGLGFTRDP
                     YIDFGTHNMWVNLGEQQFHLPQSDAQILRGEIAVVVPNLEDLAKRLKFVSKALKDTRF
                     SWEEADQRVVLSCPWGNRIVAYPAGSFPAMDLGMPYVDMSVPPGTAPGIARFYESVMG
                     CPVSVEPGRVKVAMGYNQKLIYTETEAPLAPYDGHHIAIYVSDFSGPHRFLHERGLIS
                     QESDQHQYRFQKIVDPADDRGEAEPLFELEHEVRSLGHPMYRRPLVNRNPAVNFFTYR
                     RGNEAFTPG"
     gene            1021..2121
                     /locus_tag="AC16PP_10710"
     CDS             1021..2121
                     /locus_tag="AC16PP_10710"
                     /inference="COORDINATES: protein motif:HMM:NF014305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482141.1"
                     /translation="MGQDTDVLLEATMALVPPLLTALDALAHAGRHLHPQSLAHLVPQ
                     IDAFARPLRQGARKFEEIPWPPHLERFRDHISASAGEALAAMDAMSACLEQANPVLAA
                     YQAMGAATRATERLYPVSFMLPPVSRFFLTEAGRDDGALARRLERADPGRDDVGVMHA
                     HNGPGERGGFSLYVPEYYDAGEAWPVVVALHGGSGHGRSFLWNWLKDARSRGVLLVAP
                     TSRDRTWSLAGSDADSASLDAIVERVCEHWRVDERRILLTGMSDGGTFSYLSGLRSDT
                     PFTHLSPISASFHPFILEGVDRDRLTGLPIYLVHGTLDWMFPVDVARTARDALTAAGA
                     ALTYREVDDLSHTYPREENARILDWLMGPSAP"
     gene            complement(2126..2761)
                     /locus_tag="AC16PP_10715"
     CDS             complement(2126..2761)
                     /locus_tag="AC16PP_10715"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6482142.1"
                     /translation="MLTLWGGATSRTLRAHWMLHELGLDYEPRLIGSRTGATQTDAFK
                     QLNPKEKIPVLVDDEFTLTESVAIVTYLGDTYGSGSGLVPEPFTRNRARYNEWASFIQ
                     MELDAHTLYVLRKHRDLAGIYGEAPAAVATAIGGFAKQVAVADEALRGSAHLIGSKFS
                     AADLLLVSCLDWAAAYGLELTPGLDEYRLRIRKRPAYRAAARLNFSISAGA"
     gene            complement(2768..3070)
                     /locus_tag="AC16PP_10720"
     CDS             complement(2768..3070)
                     /locus_tag="AC16PP_10720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6482143.1"
                     /translation="MIIVNAIIESDENSIAAMREGITTMVTATLEEPGCDDYCFSVEL
                     DDPTRIRITERWHSIEALAAHFQTPHMAAFQQVMAANPPKNVTAHCYDAEEIDLPR"
BASE COUNT          504 a         1015 c         1076 g          605 t
ORIGIN      
        1 agcggaaggt agtagggctc ttctgcgagc cgggcgagtt tggctgcttc gctcatccgg
       61 gagtgaaggc ctcgttgccc ctgcgatagg tgaagaagtt caccgcggga ttccggttca
      121 ccagcggtcg ccggtacatg ggatggccaa ggctgcggac ctcgtgctcc agctcgaaca
      181 gcggctcggc ctcgccgcga tcgtcggcgg gatcgacgat tttctgaaag cggtactggt
      241 gctgatccga ttcctggctg atcaggccgc gttcgtgcag aaagcggtgg ggacctgaaa
      301 aatcgctgac gtagatggcg atgtggtgac cgtcgtaggg ggcgagaggc gcttcggtct
      361 ccgtgtagat cagcttctgg ttgtagccca tggccacctt gacgcggccg ggctccacgg
      421 ataccgggca gcccatcacc gattcgtaga accgtgcgat gccaggggcg gttccgggcg
      481 ggacggacat gtccacgtag ggcattccca ggtccatggc gggaaaagat ccggcggggt
      541 aggcgacgat gcgattgccc cagggacagc tgagcaccac cctttggtct gcctcttccc
      601 acgagaagcg ggtgtccttc aacgccttgg agacgaactt cagtcttttc gcgagatctt
      661 cgaggtttgg aacgaccact gcgatctcgc cgcgaaggat ctgggcgtcc gactggggga
      721 gatgaaactg ctgctcaccg agattgaccc acatgttgtg cgtgccgaag tcgatgtacg
      781 ggtcgcgggt gaagccgaga ccggtcacgt agaacagcgc ggccagcccc tgatcgggaa
      841 cggtcacgtt gacgtgctcc agcatctgca cgttgccgac gtcctcggcg ctgcggtcgt
      901 actcgggcaa ttgacgatct tcgagtggcg caacccatga ctttaatcaa tctgccgacg
      961 ccgatccaac gcggcgggcg ccggcgctca gccggtccat cggcagggta tagtgcgctg
     1021 atgggccaag acaccgacgt cctgctcgaa gcaaccatgg cgctggttcc accgctgctc
     1081 accgcgctgg atgccctcgc ccatgcgggc aggcacctgc atcctcagtc cctggcgcat
     1141 ctggttcctc agatcgacgc gttcgcccgg ccgctccggc agggcgcgcg caagttcgaa
     1201 gagatcccgt ggccgcccca cctggagcgg tttcgcgatc acatcagcgc ttctgcgggt
     1261 gaagcgctcg cggccatgga cgcgatgagc gcgtgcctag agcaggcgaa cccggtgctc
     1321 gccgcctacc aggccatggg tgccgcgacc cgcgctaccg aacgcctgta cccggtctcc
     1381 ttcatgctcc ccccggtcag ccgctttttc ctgaccgagg ccggccgcga cgacggcgcg
     1441 ctggcgagac gcctggagcg ggcggatcct ggccgggacg acgtgggggt gatgcatgcg
     1501 cacaacggac ctggtgagcg gggcggtttt tccctctacg ttccggagta ctacgacgcc
     1561 ggcgaggcgt ggcctgtggt ggtcgccctg cacggcggca gcggtcacgg gcgcagcttt
     1621 ctctggaact ggttgaagga tgcccgctca cggggcgtgc tcctggtcgc gcccacctcc
     1681 cgcgaccgaa cgtggtcgct ggcaggctcc gatgcggaca gcgcgagcct ggacgccatc
     1741 gtcgaacggg tctgcgagca ttggcgcgtg gacgagcgcc gcatactgct gacgggcatg
     1801 tccgacggcg gaaccttcag ctatctgagc ggcctgcgct cggatacccc gttcacccat
     1861 ctctcgccca tctcggccag ctttcatccc ttcattctgg aaggcgtgga tcgggatcgc
     1921 ctgaccggcc tgcccatata cctcgtgcac gggacgctgg actggatgtt ccccgtcgac
     1981 gtggcgcgca cggcgcgcga tgccttgacc gcagctggag ccgcgctcac ctaccgcgag
     2041 gtcgacgatc tatcccacac ctatccccgg gaggagaacg cccgcatcct ggactggctg
     2101 atggggcctt cggctccctg agggctcagg ctccggcaga tatagagaag ttgagccggg
     2161 ccgcagcccg gtaagcgggc cgcttgcgga tccgcaaccg gtactcgtcg aggccgggcg
     2221 tgagctcgag cccgtaggcg gcggcccagt ccaggcagct gaccagcagc aggtctgctg
     2281 cgctgaactt gctgccgatc agatgagccg accccctgag cgcttcgtct gccacggcca
     2341 cctgcttggc aaacccgcca atggcggtag ccaccgcggc gggggcttcg ccgtagatgc
     2401 ctgccaggtc ccggtgtttg cgcaggacgt agagcgtgtg cgcgtcgagc tccatctgga
     2461 tgaagctggc ccattcgttg tagcgcgccc ggttccgggt gaaaggctca ggtacgagac
     2521 cgctgccgga gccgtaggtg tcgcccaggt acgtgacgat ggccacgctc tcggtgagcg
     2581 tgaattcgtc gtccaccagc acgggtatct tctccttggg gttgagctgc ttgaaggcgt
     2641 ctgtctgggt ggcgcccgtg cgcgagccga tcaggcgggg ctcgtagtcg agccccagct
     2701 cgtgcagcat ccagtgcgcc cgcagcgtgc ggctggtcgc gcccccccaa agcgtcagca
     2761 cgacggctca gcgcggcagg tcgatttctt cggcgtcgta gcagtgggcg gtgacgttct
     2821 tcggcgggtt ggcggccatc acctgctgaa acgccgccat gtggggcgtc tgaaaatggg
     2881 cggccagcgc ttcgatgctg tgccagcgct cggtgattcg gatcctggtc ggatcgtcga
     2941 gctccacgct gaagcaatag tcgtcgcatc cgggctcctc gagcgtggcc gtcaccatgg
     3001 tggtgatgcc ttcgcgcatg gcggcgatgc tgttttcgtc cgactcgatg atggcgttga
     3061 cgatgatcat tgtccctccc agagtttcgt ctgttccgtt ctgcgctctc gagtgaaggt
     3121 ctccatggcg tagtcgacgc gatcctcgac gttgtgcgcg aaagactcga gattctctat
     3181 gtgtcgcagg cgcggcacca
//
LOCUS       DBNHKV010000338        17597 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_685864, whole genome shotgun sequence.
ACCESSION   DBNHKV010000338 DBNHKV010000000
VERSION     DBNHKV010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 17597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17597
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_685864"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="AC16PP_10725"
     CDS             complement(<1..867)
                     /locus_tag="AC16PP_10725"
                     /inference="COORDINATES: protein motif:HMM:NF004843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6482144.1"
                     /translation="MNDFEGKTAVVTGAASGIGLALAERFAAAQMQVVLADIEEAALD
                     QAVKRLEERQARVIGVVTNTMVESSVAELAERALGEFGNVHVLCNNAGVASLGAGGRG
                     IWEVPAADWDWVMGVNFYGVLFGIQAFVPHMLEHGEPGHIVNTASLAGLFPGGGTYGV
                     SKHAVLALTETLYNDLKARGAALSASVLCPGFVSTRIAKAERNRPAGLEIDQGDVSSE
                     MAAAAEALIAQGKDPAEIAEAVFEAIAQDNLYVLPHPAWDDFVRARVDHVLDRRGPAT
                     MDPAELMRRRAGG"
     gene            complement(907..2718)
                     /locus_tag="AC16PP_10730"
     CDS             complement(907..2718)
                     /locus_tag="AC16PP_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012946.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6482145.1"
                     /translation="MSRSEVEGAEFGFDPEALRAKYAEERDKRLRADGNAQYLEVTGK
                     FAHFLDDPYVERTPRDALTDEVEVAIIGGGFGGLLAGARLREAGVEDLRIIEKGGDFG
                     GTWYWNRYPGAQCDIESYIYLPLLEEVGYIPREKYSFAPEIMAHSQAIGRKYDLYRNA
                     CFQTEVTELRWDEEDQRWEIRTNRGDRMKARFVCMSNGPLNRPKLPGIEGIESFQGHT
                     FHTSRWDYAYTGGDPTGNLENLAGKRIGIIGTGATAVQCVPHLAETSEHLHVFQRTPS
                     SIDARGNRPTDPGWAAKLAPGWQRDRMHNFNVLVTGGYQEEDLVSDGWTDIIRNLGII
                     SARRAQTESLTLEDMAKNVELADFQKMEQIRARVDELVDDPETAEALKPYYRQFCKRP
                     CFHDEYLQAFNRPNVTLVDTDGRGVERITDKGIVANGVEYELDCIVFATGFEVGTSYS
                     RRAGYEIVGRNGLTLTDKWADGMKTLHGFQTNGFPNCFFMGVTQGGFTANFPHLLNEQ
                     SIHIAYMIDQARNKNAHVLEASPEAEAGWVETIRQMAVFNQNFLESCTPGYYNNEGNP
                     EVARSLLGSQYGGGPEAFFQILRDWREEGGMEGLDFS"
     gene            3026..3694
                     /locus_tag="AC16PP_10735"
     CDS             3026..3694
                     /locus_tag="AC16PP_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6482146.1"
                     /translation="MFKLTDANLVFRALVIFGLVLFGFFLAEQQGLVSLALGSDKSRI
                     SYLILGLYGAVSVHWLWLARALGAERRRFVALENSICAGEAVDVRGGLLGGFLENLSL
                     KRDDDASALLEAFGDELANRHAFGHFASDVLLKLGLLGTVVGFILMLLPVGEIEEFDP
                     SLMQQLLSAMSGGMAVALYTTLAGLVTSTLLKLQYHLLDASAAQLVTRLAVLVDVRLK
                     RDAA"
     gene            3684..4301
                     /locus_tag="AC16PP_10740"
     CDS             3684..4301
                     /locus_tag="AC16PP_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482147.1"
                     /translation="MRHDVFRQRESQDAFTDLLFNALLGFAFMFVAAFALISDPSNTG
                     KIDSKAEVLITVRWADRHPDDVDTLVEGPRGQLVWYHNRDSGLMHLDRDDRGLFADRI
                     VLDGREVSNPINQETVTVRALQPGEYVVNVLHYQSNYSEPLPVTVKVEKLNPTVTLVY
                     YGTRQLTGVGDEQTAVRFTIGADGQVASTSELPKPLLTRAVGGKT"
     gene            4298..4864
                     /locus_tag="AC16PP_10745"
     CDS             4298..4864
                     /locus_tag="AC16PP_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482148.1"
                     /translation="MTGPILGLTLAYVAVAALLLCLNLATRFSPWVKGIAVVLVTALY
                     ASAWLGYQGLLGWASPAALPETFRVLWITVEEPDKESGAPGTIFFWVRELDEAGFPVA
                     PPRAHRIAWDEASARAAQEALEKLEEGERLNGRLSRGVVDEGEGRPEETTGYAAEQSV
                     TGAVGRRPDFEFVQVPPPALPPKAVPGG"
     gene            complement(4868..7090)
                     /locus_tag="AC16PP_10750"
     CDS             complement(4868..7090)
                     /locus_tag="AC16PP_10750"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003239772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HIR6482149.1"
                     /translation="MRAFIWIALVAIAAVVYFFATSTAPDGATEDGPTAAGGGTLESG
                     PSVGLIDDARINAADSEPGNWLAYGRTYEEQRFSPLTQINRESVGELGLAWYKDMGTN
                     RALEATPIVVDGIMFFTTTWSRVHAVDARTGETVWFFDPEVPGETGRKACCDVVNRGV
                     AVYQGKVFVGSLDGRLIALDAATGEKVWEVDTIVDRTRAYTITGAPRAARGKIFIGNG
                     GAEYGVRGYVTAYDAQTGEQVWRFYTVPGDPSKPFEHPEMEMAAKTWKGGEWWEIGGG
                     GTVWNSIVYDPDFNHVYLGVGNGSPWTRVIRSPGGGDNLFLSSIVALNADTGEMQWYY
                     QTTPGDNWDYTAVQDIALADMEVDGEMRKVLLQAPKNGFFYVIDRTNGELLRANPYVA
                     VNWATHVDMATGRPVENPAMDYADTAKWILPGPLGGHNWQAMSVDVEAGLVYIPALEN
                     PLIYGMPDEWQQTGVYKRDPGRWNTGIELGAIAQLFVDNIAEQPTPRGLLKAFDPLSG
                     EERWSVEIPHYWNGGVLGTAGGLVFQGNAMGMFKAYDKSTGEALWEFNTYTSMLAPPV
                     TFELDGEQYVSILTGFGGGDLFGGEPLPPLSMHASLKYNNHGRLLVFKLGAKEQLPVP
                     SARDRTIPEQLVADVDVADIARGERLYNDYCAVCHGLVARSSGAIADLRLMSAESHDA
                     FDGVVLEGLMSARGMTAFSDVLTVEDSERIHHYVRARAHEDREVALGNSEAARLTWLN
                     "
     gene            7236..7412
                     /locus_tag="AC16PP_10755"
     CDS             7236..7412
                     /locus_tag="AC16PP_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482150.1"
                     /translation="MKSWIDFRENGSSPLTKIKREYFGELRLVWKEELGNYHVRQATA
                     LVGGGGRSREVSFD"
     gene            7405..8202
                     /locus_tag="AC16PP_10760"
     CDS             7405..8202
                     /locus_tag="AC16PP_10760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6482151.1"
                     /translation="MTEEASRFVGSIPEHYDAGLGPHIFEGYAEDLAGRVAALDPAAV
                     LELAAGTGIVTRRLRDVLPAGCDIVASDLNQPMLDFAEAKFAPDESVGFEVADATDLP
                     WQDERFDVVTCQFGVMFFPDKHRSYAEARRVLRAGGHYVFNVWDAWPFNAFAEITHDV
                     VAGFFPDDPPGFYRVPFGYHDVEVIRRSLLDAGFRAASAETVQLTSAIPSAENFARGL
                     VFGNPLFQEIEQRGGDADAIHAAVADAVARRLGSQMQIRAIVVHAQK"
     gene            complement(8232..8729)
                     /locus_tag="AC16PP_10765"
     CDS             complement(8232..8729)
                     /locus_tag="AC16PP_10765"
                     /inference="COORDINATES: protein motif:HMM:NF016213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HIR6482152.1"
                     /translation="MDSAMLIDVLLWILVVGLVIVGVAGLLLPALPGAPLVFAGLLIG
                     AWIDDFAYVSWGTLITLGIMAALTYAVDFAAGALGASRFGASPRAVWGAVIGGIVGIF
                     MGLVGIIIGPFVGAVIGELSAQRSLEEAGRAGLGATLGLVIGTAIKLTLAMAMVGIFV
                     LMRFL"
     gene            complement(8801..9715)
                     /locus_tag="AC16PP_10770"
     CDS             complement(8801..9715)
                     /locus_tag="AC16PP_10770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6482153.1"
                     /translation="MQIGIHAPHLGRELDPVTVRSFVQECDTLGVHSLWVSDHVCWPA
                     DIASKYPYTEDGSFPATPEMGWLDPIGTLLFIAGCTDRVRLGTTVLILPYRPPVATAK
                     QLATLDVVSGGRLILGVGIGWMAEEAAVLGMPWDHRGRRSDEQLEIFRRLFTETEPSF
                     EGEFYSFPKVGFEPKPVQQPVPVWVGGSTPAAFRRVARFGQAFHAAFQPLAEVGREWR
                     AVQAACEEIGRDPSDLSLSLRLYLDPDSRMEPEKSVAGSAEQMRERLFELAAAGVGHV
                     VLDPVARGGVQGRLDAVRGFMTDVAPAS"
     gene            9840..10466
                     /locus_tag="AC16PP_10775"
     CDS             9840..10466
                     /locus_tag="AC16PP_10775"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6482154.1"
                     /translation="MLKLYLMPGSCALASHITLREAGADFELKVVDFMSGEQRTPEYR
                     KVNPKGRVPALVTDRGVLTETPAILLYVAQTHPEARLAPLDDPFELARLQAFTSYLCA
                     TVHVAHAHSRRGERWADDESAIEAMKRKAPGNMADCFQLIEDQFFEGPWLMGEQFTVA
                     DPYLFTITRWLKFHQIDSSGYPRVHDHLGRMGERPAVRAALEAEAAAA"
     gene            10704..11147
                     /locus_tag="AC16PP_10780"
     CDS             10704..11147
                     /locus_tag="AC16PP_10780"
                     /inference="COORDINATES: protein motif:HMM:NF025691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HIR6482155.1"
                     /translation="MEEVMKQRLTSRNLWRRVLYMLLFVLAYGIAEAVVAVVVVVQFF
                     FILISGRANENLLVFGNNLSTYVRQILQFQTFNSEYLAYPFGDWPDEGLGSELWLEEG
                     TAEEAEAAPPVAGSEPSDEAEPDSGVAERGPIEGSADPEEPDPRG"
     gene            complement(11164..11496)
                     /locus_tag="AC16PP_10785"
     CDS             complement(11164..11496)
                     /locus_tag="AC16PP_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482156.1"
                     /translation="MNQRVVAGILALALCLHALPASARLYSYVNEEGDYVVSQKKPKN
                     VDEYAVLTDEGEFIRLVKRKRDDVPITHWRPWYMPNEPDPINPVEPGLEQRTPEVLIE
                     EVDGPEPN"
     gene            complement(11551..14913)
                     /locus_tag="AC16PP_10790"
     CDS             complement(11551..14913)
                     /locus_tag="AC16PP_10790"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6482157.1"
                     /translation="MNGPIAWFARNHIAANLLLAVMVLAGLLSLPIMPQKDFPDLDYK
                     LITVSVRYLGAAPEEVEDGVCIRIEEAIEGLQGIERIRSNAVEGRCTVNVELFENADD
                     NKVLGDVKNRVDAIETFPQEAEKPIITLVAAERTVVEIAVTGPSDERELKSIAQQVRD
                     DLAALPGITRVDVSGVRPYEISIEISEATLRRYGLSFDEVANAVRAASLDLPGGAIKT
                     ESGEVLLRTEGQAYRGSEFEDLVILTRGDGTRVFLRDLATVVDGFEDTDERVWFDNKP
                     AALVQVFRVGDQDILAVTRAVKSYLDDADERLPQGVDLTIWNDNSEFMRDRLDTLMNS
                     ARQGFMLVLLMLALFLRPRLAFWVSVGVPAAFLGAIFVANLAGLSIDGISTFGFILVL
                     GILVDDAVVIGERVHSLQSRAGESPPSETLISAAIEGTRQVSMPVIFGVLTTITAFLP
                     LTFGPGPTGQIQSVISVIVLCCLTFSLIESQLVLPAHLGHRRVQTAAGEVGLMMVPLV
                     AIVLMEFAWDLRSFFAIGIGTTAVLIAWSIQGGFERFAARVIALQKQFSDRLEHVIQF
                     RFRRLVSAATDARYVTAAGAVAILGITVGLVAGGHMPFTYFPSLPADRISAKLTMPLG
                     TPARVTEEIVTNIAVTAEVLQRELASEQPDQPPVRFILAAVGAHPVSSAGLGAVIAPP
                     AARTGGHMGEVTLQLTPSDERDLSTLEIANRWRDATGPIPDAVEFTFATSTFSVGSEI
                     DIQLAGPDLEELANVAAEIRSELAAYPGVIDITDSFRSGKRELKLDILPAGEALGLNL
                     GALARQVRQAFYGEEIQRIQRGRDDVRVMLRYTEAERRALGSLDEMRIRTRNGSEVPF
                     ATVASADLGRGFSAIKRTDGRRVVNVTADVDRNVITANEVLADFGPGPLRTILRDHPR
                     VSFSLEGTRREQEDSIASLLPMFLMALAVIYALLAVPLRSYTQPLIIMSVIPFAMAGA
                     VWGHMIMKSLGLLSNIVIMSVTGGIAACGVVVNSSLVLVHGVNARRREGMDIHSAVVE
                     AAVTRCRPILLTSLTTFAGLSPLLLNQSVQAQILIPMATSVAWGVVFATVVTLLVVPV
                     GYLILDDLGRKLSRGRTSVSPSSADAGASAGR"
     gene            complement(14910..16073)
                     /locus_tag="AC16PP_10795"
     CDS             complement(14910..16073)
                     /locus_tag="AC16PP_10795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6482158.1"
                     /translation="MNRRLQAALVLLGGFAVAVLVFLSGPKMQPQAPSAIPPLVRVIE
                     AEPREVQMTSHTHGTVVPRTESDLVPEVDGRVTAVSPSLVSGGFFNQGDVLLEIEPVD
                     YEVALEHARAGLARAESDLENARKSLTRIQDLVRRGATSDSDRDDAANRERIAQATLR
                     EARARLSRAERDLDRTRLVAPYQGRVRNERVDVGQFVKRGNPVATIYAVDFAEVRLPI
                     QDEELAFLDLPLGYTGDESSIPIPVTLRARFAGVEHEWQGEVVRTEGELDPATRMVHV
                     VARVPEPYVSRDGRPPLSVGLFVDADIQGKTLSDVVILPRSALRGGDRVLVVDQDERL
                     RFRPVDVYRVAEEEVYIVGGLARGERVCISPLQSTSDGMLVRVADVEQRAAPS"
     gene            complement(16070..16759)
                     /locus_tag="AC16PP_10800"
     CDS             complement(16070..16759)
                     /locus_tag="AC16PP_10800"
                     /inference="COORDINATES: protein motif:HMM:NF037008.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6482159.1"
                     /translation="MAQASRPLRADSAATRESILEASVKLFGERGYRNTSLRAITDAA
                     DVNQAAANYHFGSKAQLLDAAFERCIAPINDERMRRLRQLQASSSSPSVEALVRAFVD
                     LEFALTDNEHLDQFLAHLFAEPKSVSVPLLEKTFAPVVQAYFAALHAALPDVPVEVLR
                     WRFHLMIGSMIQLARFRHPLNFFQADSPPAEGSLQAGIDQLVEFVVAGLTQPGTSGDG
                     ASGDGASGDGE"
     gene            16908..17282
                     /locus_tag="AC16PP_10805"
     CDS             16908..17282
                     /locus_tag="AC16PP_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6482160.1"
                     /translation="MLGYATIGVNDMDRAVAFYDALLAELGAKQLMGMDRIKMYGTSP
                     DAGMLAICIPYDEEPQSPGNGNMVAISAGSREAVDRLYAKAMELGAADEGEPGERNPV
                     FYGAYVRDLDGNKLCFYEMKLG"
     gene            17379..>17597
                     /locus_tag="AC16PP_10810"
     CDS             17379..>17597
                     /locus_tag="AC16PP_10810"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6482161.1"
                     /translation="MFPRAVVASVDIQAPAREVWAVLVDFARYPEWNPFTPEVEAELA
                     VGAPVRLRVSMPGRRELERVEQINRVEPE"
BASE COUNT         2956 a         5705 c         5804 g         3132 t
ORIGIN      
        1 gccgcccgct cgtctgcgca tgagctcggc cgggtccatg gttgcggggc cgcgtctgtc
       61 cagcacgtga tcgacccggg cgcgaacgaa gtcgtcccat gccggatggg gcaggacgta
      121 gagattgtcc tgggcaatgg cctcgaagac ggcttccgcg atctcggcgg gatcctttcc
      181 ctgggcgatc agggcttcgg cagcggccgc catctcgctg cttacgtcgc cttggtcgat
      241 ctcgagcccc gcgggacggt ttcgctcggc cttggcgatg cgggtgctga cgaatccggg
      301 gcagaggacg gaggcgctga gcgccgcgcc ccgtgccttg agatcgttgt acagggtttc
      361 cgtcagggcg agcaccgcat gcttgctcac cccgtaggtg cccccgccgg gaaagaggcc
      421 ggccagcgag gcggtgttga cgatgtgccc cggctcacca tgctcgagca tgtgcggcac
      481 gaaggcctgg attccgaaga gcaccccgta gaagttcacg cccatgaccc agtcccagtc
      541 ggcggccggc acctcccaga tgccccggcc cccggcgccg aggctcgcca caccggcgtt
      601 gttgcagagc acgtgaacat tgccgaactc cccgagcgcg cgttcggcca gctcggccac
      661 ggacgactcc accatggtgt tggtgaccac accaatcaca cgcgcctgcc gctcttcgag
      721 tcgtttgacc gcctggtcca gcgcggcctc ctcgatgtcc gccagcacca cctgcatctg
      781 cgcggccgcg aaccgttccg ccagggcgag gccgatgccg ctcgcggcgc cggtcaccac
      841 tgccgtcttg ccttcaaagt cgttcatgtc cgctccctct atggtcgaca cccggcgcga
      901 gaatcatcag gaaaagtcga gaccctccat gcccccctcc tcgcgccagt ccctgagaat
      961 ctgaaaaaac gcctccggcc cgccgccgta ctggctgccg agcagcgatc gggccacctc
     1021 cgggttgccc tcgttgttgt agtagccggg cgtgcaggat tccagaaagt tctggttgaa
     1081 aaccgccatc tgccgaatgg tctctaccca gcctgcctcc gcttcggggg aggcttccag
     1141 aacgtgggca ttcttgtttc tcgcctgatc gatcatgtaa gcgatgtgga tgctctgctc
     1201 gttcagaaga tggggaaagt tggcggtgaa gccgccctgg gtcacaccca tgaaaaagca
     1261 gttcggaaaa ccgttggtct gaaaaccgtg taaggtcttc atgccgtcgg cccacttgtc
     1321 agtcagcgtc aggccgttcc ggccgacgat ctcgtagccc gccctgcggc tgtacgaggt
     1381 gcctacctcg aagccggtcg cgaagacgat gcagtcgagc tcgtactcca cgccgtttgc
     1441 cacgatccct ttgtccgtga tgcgctccac gccccttccg tccgtgtcca ccagggtgac
     1501 gtttggacgg ttgaatgcct gcaggtactc gtcgtgaaag cacggtctct tgcagaactg
     1561 tcggtagtag ggcttgagag cctcagccgt ttccggatcg tcgacaagct cgtccacgcg
     1621 cgcccggatc tgctccatct tctggaagtc ggccagctcc acgttctttg ccatgtcctc
     1681 gagcgtcagg ctctcggtct gagcgcggcg ggcggagatg atgcccaggt tgcggatgat
     1741 gtcggtccag ccatcgctga ccagatcttc ctcctgatag ccgccggtca cgagcacgtt
     1801 gaagttgtgc atgcggtccc gctgccagcc cggcgcgagc tttgccgccc agcccgggtc
     1861 ggtaggccgg ttgccgcgcg catcgatgga tgatggcgtt cgctggaaca cgtgaaggtg
     1921 ttcagaggtc tcggccagat gcggcacgca ctgaacggcg gtggctccgg tgccgatgat
     1981 gccgatgcgt ttgcccgcca ggttctcgag gttgccggtc gggtcgccgc cggtgtaggc
     2041 gtagtcccag cggctggtgt gaaacgtgtg gccctggaac gattcgatgc cttcgatgcc
     2101 gggcagcttc ggccggttga gcggaccgtt ggacatgcag acgaaacgag ccttcatccg
     2161 atcaccgcgg ttagtgcgga tctcccaccg ctggtcctcc tcgtcccagc gaagctccgt
     2221 gacttcggtc tggaagcagg catttcgata aaggtcgtac ttgcggccga tggcctggct
     2281 gtgggccata atctccggcg cgaagctgta cttctccctg gggatgtagc cgacttcctc
     2341 gagcagcggg agatagatgt acgactcgat gtcgcactgg gcgcccgggt aacggttcca
     2401 gtaccacgtg cccccgaaat ccccgccctt ctcgatgatc ctcagatcct cgacgcctgc
     2461 ttcccgaagc cgggcgccgg caagcaggcc gccaaacccg ccgccgatga tggcgacttc
     2521 gacctcgtcg gtcagggcgt cccttggtgt gcgctccacg tagggatcgt ccaggaaatg
     2581 ggcgaacttg ccggtaactt ccaagtactg ggcgttgccg tcggcgcgca ggcgcttgtc
     2641 ccgctcctct gcgtattttg ctcgcagggc ttccgggtcg aacccgaact ccgccccttc
     2701 gacttctgac ctgctcatgg gtcgacctcc aactgcacgc ggaaaagcgg cctatttagg
     2761 aatgccgcta tgtagattgc aaccttcgct ggcgggagcg tatgcggccg tcggggttgg
     2821 acggggatgc ggcttcttga cacctgccgg cgtccttggt cgcattgtgc agtccatcgg
     2881 tcggcgggga ggggcaccgt gattggggga agctgtcagt ttggtgccgt gagctttgag
     2941 cttgcggctg atccagcgag catttgcgag ctgcccgtcc ggcatatcga cggggctgaa
     3001 acccggcagt tcgtggatta gggccatgtt caagctcaca gacgccaacc tcgtctttcg
     3061 cgccctcgtc atcttcggtc tcgtgctgtt cgggtttttt ctggccgagc agcagggcct
     3121 ggtgagcctg gctctcggct ccgataagag ccgcatctca tacctcattc tcggtcttta
     3181 cggcgctgtg agcgttcact ggctgtggct ggcccgcgcg ctgggtgcgg aacggcggcg
     3241 cttcgtcgcg ctggagaaca gcatctgcgc aggtgaggcc gtcgacgtca gaggcggtct
     3301 gctcggcggg tttctcgaga acctctccct caagcgcgat gacgacgcct cggctctgct
     3361 cgaagcgttt ggcgacgagc tggcgaaccg gcacgcgttc ggtcatttcg ccagcgacgt
     3421 tctgttgaag ctcggcctgc tcggcaccgt ggtcggcttc atcctcatgc tcctgcccgt
     3481 cggggagata gaagagttcg acccgagcct catgcagcag ctgctgagtg ccatgagcgg
     3541 cggcatggcg gtagcgctct acacgacgct ggccgggttg gtcaccagca ccctgctcaa
     3601 gctgcaatat cacctgctcg atgcgtcggc cgctcagctc gtcacgcggc ttgcggtgct
     3661 ggtggacgtg aggctgaagc gcgatgcggc atgacgtctt ccggcagcgc gaatctcagg
     3721 acgctttcac cgacctgctt ttcaacgcgc tgctgggctt cgccttcatg ttcgtcgccg
     3781 cgttcgccct gatcagcgat ccgagcaaca cggggaagat cgactcgaaa gccgaggtgc
     3841 tcatcaccgt gaggtgggcc gaccggcacc cggacgatgt cgataccctg gtcgaggggc
     3901 caagagggca gctggtctgg taccacaatc gcgacagcgg gctcatgcac ctggaccgcg
     3961 acgatcgggg cctgttcgcg gatcggatcg ttctcgacgg ccgggaggtt tccaacccga
     4021 tcaatcagga gacggtgacc gttcgcgcgc tgcagccggg tgagtacgtg gtgaacgtgc
     4081 tccactatca gtcgaactac agcgagccgc tgcccgtcac cgtgaaggtg gagaagctca
     4141 accccacggt tacgctcgtc tactacggca cccggcagct caccggggtg ggggacgagc
     4201 agacggccgt tcggttcacc atcggcgccg acggacaggt cgcgagcacc agcgagctgc
     4261 cgaagccgct cctgacccgg gcagtgggcg gcaagacgtg accggcccga ttctcgggct
     4321 caccctggcc tacgtggcag tggccgccct gctcctgtgc ctgaacctgg cgacccgctt
     4381 ctcgccgtgg gtcaagggaa ttgcggtggt gttggtcact gcgctttacg cgtcggcctg
     4441 gctcggctac cagggtctgc tcggctgggc atcgcctgct gcgctgccgg agacgttccg
     4501 cgtgttgtgg atcacggtgg aggagccgga caaggaaagc ggcgcccccg gcaccatctt
     4561 tttctgggtg cgtgagctgg acgaggcggg ttttccggtc gcccccccca gagcgcaccg
     4621 gattgcctgg gacgaagcgt ctgccagggc ggcccaggag gctcttgaaa agctggagga
     4681 gggggagcgg ctcaatgggc gtttgagccg aggcgtggtg gacgagggcg aaggccgtcc
     4741 cgaagagacc accggctacg cggccgagca gtccgtgacg ggcgccgttg ggcgccgccc
     4801 ggacttcgag ttcgtgcagg tcccgccgcc ggcgctgccg cccaaggcgg tcccgggcgg
     4861 gtagcggtca gttcagccag gtgagcctgg cagcctccga gtttccgagc gccacttccc
     4921 ggtcctcgtg tgcccgggct ctgacgtagt ggtgaatgcg ttcggaatcc tccaccgtga
     4981 gcacgtcgct gaacgccgtc atgccccttg cggacatcag cccctcgaga acgaccccgt
     5041 cgaaggcgtc gtgggactcc gcggacatca ggcgcaaatc ggcgatggcc ccgctggagc
     5101 gtgcgaccag cccgtggcag acggcgcagt agtcgttgta cagacgctcg ccccgggcga
     5161 tgtccgccac gtcgacgtcg gcgaccagct gctccggaat ggtccggtca cgggccgagg
     5221 gaaccggcag ctgctctttg gcgccaagct tgaacaccag caaacggccg tggttgttgt
     5281 acttcagcga agcgtgcatc gacagcggcg gcaggggctc gccgccgaac aggtcgccgc
     5341 cgccgaaccc ggtgaggatc gatacgtatt gctcgccgtc cagctcgaaa gtgacgggtg
     5401 gtgcgagcat ggaggtgtaa gtgttgaact cccacagcgc ctcgccggtg gatttgtcgt
     5461 aggccttgaa catccccatg gcgttgccct ggaagaccag cccgccggcg gtgccgagca
     5521 cgccgccgtt ccagtagtgt gggatctcca ccgaccagcg ctcctcgcct gagagcgggt
     5581 cgaaggcttt gagcagaccg cgaggcgtgg gctgctcggc gatgttgtcc acgaacagct
     5641 gcgcgatggc gccgagctcg atccccgtgt tccagcggcc cgggtcgcgc ttgtagacgc
     5701 ccgtctgctg ccactcatcc ggcatgccgt agatcagcgg gttctccagc gccgggatgt
     5761 agaccaaacc ggcctccacg tccacggaca tggcctgcca gttgtgcccg ccgagcggtc
     5821 ccgggagtat ccacttcgct gtgtcggcgt aatccatggc cggattttcc actggccggc
     5881 cggttgccat gtccacgtgg gtggcccagt tcaccgcgac gtagggattg gcgcgcagaa
     5941 gctcgccgtt ggtccggtcg atgacgtaga agaagccgtt cttgggcgcc tggagaagca
     6001 ccttgcgcat ctctccgtcc acttccatgt cggcaagcgc gatgtcctga acggccgtgt
     6061 agtcccagtt gtcgcccggg gtggtctggt agtaccactg catctcgccg gtgtccgcgt
     6121 tcagggcgac gatggaggag aggaacaggt tgtcgccgcc gcccggtgat cggatcaccc
     6181 gcgtccaggg ggaaccgttg cccacgccga ggtagacgtg gttgaagtcc gggtcgtaga
     6241 cgatggagtt ccacaccgtg ccgccaccgc cgatctccca ccactcgccg cccttccagg
     6301 tcttcgcggc catctccatc tccggatgct cgaagggctt ggagggatcg ccgggcaccg
     6361 tgtagaagcg ccagacctgc tcgccggtct gggcatcgta ggcggtgacg tagccgcgca
     6421 cgccgtactc ggcgccgccg ttgcctatga agatcttgcc ccgtgccgct cgcggcgcgc
     6481 cggtgatggt gtaggcccgg gtacgatcga cgatggtgtc cacttcccat accttctcgc
     6541 cggtggccgc gtccagcgcg atgagccggc cgtccaggct gccgacgaag accttgccct
     6601 gataaacggc gaccccgcgg ttgaccacgt cgcagcaggc tttgcgtccg gtctctcccg
     6661 gcacctcggg atcgaagaac cagaccgtct ccccggtccg tgcgtccacc gcgtggacgc
     6721 ggctccaggt ggtggtgaag aacatgatgc cgtccacgac gatgggcgtg gcctccagcg
     6781 cacggttggt gcccatgtcc ttgtaccagg cgaggcccag ctccccgacg ctttcccggt
     6841 tgatctgagt cagcggcgag aagcgctgct cctcgtaggt gcgcccgtag gccagccagt
     6901 ttccgggctc gctgtcggcg gcgttgatgc gcgcgtcgtc tatcagcccg acggacggcc
     6961 cagattccag agtgccgccg ccggccgcgg tcggcccatc ctccgttgcc ccgtcaggag
     7021 cggtggatgt cgcgaaaaag tagaccacag cagcgatcgc gaccaatgcg atccagatga
     7081 atgcgcgcat gatgtctccc tttacctgcg tcggggatca taccgccgct ctcccgaccg
     7141 cgacaaacct cgtgcgcggc gtatgccgcg gggttcgcgc tgcgcgtctg gcgggcgtgt
     7201 ggttcgaggg atagactgga attgggagga ttgccatgaa gagctggatc gactttcgcg
     7261 agaacggctc ctcgccgctg actaagatca agcgggagta cttcggggag ctgcgtctgg
     7321 tctggaaaga ggagctcggc aactaccatg tccggcaggc gactgcgctg gtgggcggcg
     7381 gcggacggtc ccgggaggtg agctttgact gaagaagctt cccggttcgt cggctccata
     7441 cccgagcact acgacgccgg cctgggtccg cacatcttcg aaggctacgc cgaggacctg
     7501 gccggtcggg ttgccgcgct ggatcccgcc gccgttctcg agctcgcggc agggacgggg
     7561 atcgtcaccc gacggctccg cgacgtcctt ccggcgggtt gcgacatcgt cgcgtccgac
     7621 ctcaaccagc ccatgctgga tttcgctgag gcgaagtttg cgccggacga aagcgtgggg
     7681 ttcgaggtcg ccgatgccac cgacctgccg tggcaggatg agcgcttcga cgtggtgacg
     7741 tgccagttcg gggtcatgtt ttttcccgac aaacaccgtt cctacgccga ggcccgccgg
     7801 gtgctgcgcg cgggcggtca ctacgtcttc aacgtctggg atgcctggcc gttcaacgcc
     7861 ttcgcggaga tcacccacga cgtggtggcc ggcttcttcc ctgacgatcc ccccggtttc
     7921 taccgcgtgc ccttcggcta ccacgacgtc gaggtcattc gccgctcact gctcgacgcc
     7981 ggcttccggg ccgcgtccgc ggagacggtc cagctgacgt ctgccatacc ctcggcggag
     8041 aacttcgcgc gaggcctggt gttcggtaac ccgctgtttc aggaaatcga gcagcgcggc
     8101 ggcgatgcgg atgcgatcca cgcagctgtc gcggacgccg ttgcccggcg tctgggcagc
     8161 caaatgcaga tccgcgccat cgtcgtgcac gcgcagaagt agacgtctgc ctgataaggt
     8221 gcccggcgcc gtcagagaaa gcgcatcagg acgaagatgc ccaccatggc cattgccagg
     8281 gtcagcttga tggcggtgcc gatcacgagc cccagggtgg cgccgagtcc ggccctgccg
     8341 gcctcctcca gggagcgctg cgcggagagc tcgccgatga cggcgccgac gaagggcccg
     8401 atgatgatgc caaccaggcc catgaagatg cccacgatgc cgccgatgac ggctccccag
     8461 accgcccgcg gcgacgcgcc gaagcgtgat gcgcccaggg cgccggcggc gaaatccacg
     8521 gcgtaggtga gagcggccat gatccccaga gtgatgaggg tgccccagct cacgtaggcg
     8581 aaatcgtcga tccaggcgcc gatgagaagt ccggcgaaca ccaggggtgc ccccggcagg
     8641 gccggcagca gcagcccggc cacgccgacg atcaccaggc ccacgaccag aatccagagc
     8701 agcacgtcga tgagcatcgc agagtccatg ggcctattgt ggcgcgtcag tgctcacgga
     8761 gaaaccgttc cgcaggggcc tgcacggccg gcttcgctcc tcaggacgct ggggcgacgt
     8821 cggtcatgaa tcccctaacg gcgtccaggc gcccctgcac gcccccgcgg gcaaccggat
     8881 ccagcaccac gtggcccacg ccggcagcgg ccagctcaaa aagcctctcg cgcatctgct
     8941 ccgcgctccc ggccaccgac ttttccggct ccatgcggct gtccggatcg agatagagcc
     9001 gcagagacag gctcaggtct gagggatcgc gcccaatttc ctcgcaagcc gcctgcaccg
     9061 cccgccattc ccggccgacc tcggccagcg gctgaaaggc ggcgtgaaag gcctgaccga
     9121 atcgcgccac ccgccggaac gcggccggcg tggatccccc tacccagacc ggaaccggct
     9181 gttgcaccgg tttcggttcg aagccgacct tggggaagct gtaaaactct ccttcgaagc
     9241 tgggctccgt ttcggtgaac agccgcctga atatctccag ctgctcgtcg gaccggcgtc
     9301 cgcgatgatc ccagggcatg cccaggacgg cggcctcctc ggccatccag ccgatgccga
     9361 cgccgagaat gagccggccg cccgagacaa cgtccagggt ggccagctgt ttggctgtgg
     9421 ccaccggcgg gcgatagggc aggatcagca ccgtggtgcc cagacgtacc cggtcggtgc
     9481 atccggcaat gaagagcagc gttccgatgg ggtccagcca gcccatctcg ggcgtggcgg
     9541 gaaaggatcc gtcttcggtg tagggatatt tcgaggcgat gtctgccggc cagcagacgt
     9601 ggtcgcttac ccacagggag tggacgccga gggtgtcaca ctcttggacg aatgaacgaa
     9661 ccgtcaccgg atcgagctcg cgcccgaggt gcggcgcgtg aatgccgatc tgcattgtgg
     9721 agcctcccag tggcttcggt ttgagtctag cagaccgcag tatcggggac gcgacgtgag
     9781 cccccagccg agctaaagtg aagatcccat ctgcggttgt gggctcacag ggagatcaga
     9841 tgctgaagct ctacctcatg ccgggcagct gcgccctggc ctcgcacatc acacttcgcg
     9901 aggcgggcgc cgacttcgag ctcaaggtgg tggatttcat gtccggcgag cagcgcacgc
     9961 cggagtacag gaaggtgaat cccaagggcc gcgtccccgc gctcgtcacg gaccggggcg
    10021 tgctgacgga gacgcccgcc atcctgctgt atgtcgccca gacccatccc gaagcccgat
    10081 tggccccgct ggacgaccct ttcgagcttg ccaggctgca ggcgttcacc agctacctct
    10141 gcgccacggt gcatgttgcc cacgcccaca gccgccgcgg cgagcgctgg gcggacgacg
    10201 agagcgccat cgaggcgatg aagcgcaaag cgcctggcaa catggcggac tgcttccagc
    10261 tcatcgagga tcagttcttc gagggcccct ggttaatggg cgagcagttc accgtggcgg
    10321 atccctatct cttcaccatt acccgatggc tgaagttcca tcagatcgac tcgtccggct
    10381 atccccgggt gcacgaccac ctcgggcgca tgggggaacg tcccgccgtt cgcgcggcgc
    10441 ttgaggccga ggccgccgcg gcttgagtcg ccggaacgcc cccgcgcccg cctgtggggt
    10501 gtgcggcttc gcctcaatgt ggtgaatctc gccatcttcg aggtgggcct gtgagggcaa
    10561 ccatctgaaa atcatgggaa aaaagaattg gtaccaatct tgcgacactg cctggcaaca
    10621 gagggcccca gcggcccggg cgaactcgcc ccggtgtccg tcgaaccctc agccgtgatg
    10681 tgttggcaaa tcaaggaggc gccatggaag aagtgatgaa gcaacggctc acgagccgaa
    10741 acctctggag gcgcgtcctt tacatgctgc tcttcgtgct tgcctacggc attgccgagg
    10801 cggtcgttgc ggtggtggtg gtcgtgcagt tcttcttcat cctgatctcg ggtcgggcca
    10861 acgagaacct gctcgttttc gggaacaatc tcagcaccta tgtccgccag atcctgcagt
    10921 ttcagacctt caacagcgag tatctggctt atccgttcgg ggactggccg gacgagggtc
    10981 tgggtagcga gctctggctc gaggagggca cggccgagga ggcggaggcc gcgcccccgg
    11041 tggccgggtc agagccttcg gacgaagcgg agcccgactc cggcgtggcc gaacgcggtc
    11101 cgatcgaggg atcggctgat ccggaggagc ccgacccgag aggctgagcg cgatgggtct
    11161 cggctagttg ggctcgggcc cgtcgacctc ctcgatgagc acctccgggg tacgttgctc
    11221 cagcccgggc tccacgggat tgatgggatc cggctcgttt ggcatgtacc acgggcgcca
    11281 gtgggtgatg gggacgtcgt cgcgcttgcg cttgaccaga cgaatgaact cgccctcatc
    11341 ggtaaggacg gcgtactcgt ccacgttctt cggctttttc tgcgaaacca cgtagtcgcc
    11401 ctcctcgttg acgtacgagt agaggcgggc cgatgcgggt agcgcgtgca ggcagagtgc
    11461 gagcgcgagg atgccggcaa cgacccgctg gttcatctgg tcgatccgct ggctgttttg
    11521 ctggagcggc gtcattgtct gcatatcacc tcaccgcccg gcggaggctc cggcgtcggc
    11581 gctgcttggc gaaacggacg tccggccccg gctgagcttc ctgccgagat catcgagtat
    11641 aaggtaaccg acgggcacca ccagcagggt gaccaccgtg gcgaagacga cgccccaggc
    11701 tacggaggtg gccatgggga tgaggatctg cgcctgcacg ctctggttga gcagcagggg
    11761 tgacaggccg gcaaacgtag tgagtgacgt cagcaggatt ggccggcagc gcgttaccgc
    11821 tgcctcgacc accgcgctgt ggatgtccat gccctctcga cgccgcgcgt ttacaccgtg
    11881 caccagcacg aggctcgagt tgacgacgac cccgcaggct gcgatgccac ccgtcaccga
    11941 catgatcacg atgttggaca gcagcccaag cgacttcatg atcatgtgcc cccacacggc
    12001 cccggccatg gcaaagggga tcaccgacat gatgatcagc ggctgcgtgt acgagcgcag
    12061 cggcacggcc agcagagcgt aaattaccgc cagcgccatc aggaacatgg ggagcaggct
    12121 ggcgatactg tcctcctgct cccggcgtgt gccttccagg ctgaagctga ccctggggtg
    12181 gtcgcgcaga atggtcctca gcggtcctgg accgaaatcg gccagcacct cgttcgccgt
    12241 gatcacgttt cgatccacgt cggcggtcac gttcaccacg cggcgaccgt cggttcgctt
    12301 gatggcggag aagccgcggc cgagatcggc gctcgcaacc gtggcgaagg gaacctcgga
    12361 gccgttgcgg gtgcggatgc gcatctcgtc aagggacccg agagcgcggc gctccgcctc
    12421 ggtgtagcgc agcatcaccc ggacgtcgtc gcggccgcgc tgaatccgct gaatttcttc
    12481 gccgtagaac gcctgtcgga cctggcgagc cagggcgccc aggttcagcc cgagcgcttc
    12541 tcccgccggg aggatatcga gcttcagctc ccgttttccg gagcggaaag agtcggtgat
    12601 atcgatgacg cccgggtagg cggccagctc gctgcgaatc tcggccgcca cgttcgccaa
    12661 ctcttccaga tcgggcccgg cgagttggat gtcgatctcg gagcctacgc tgaaagtgga
    12721 ggtcgcgaag gtaaactcca cggcatccgg gatggggccg gttgcgtccc gccatcggtt
    12781 cgcgatctcc agggtgctga ggtcgcgttc gtcgctgggc gtaagctgca gcgttacctc
    12841 ccccatgtgc cctcccgtcc tggcggccgg aggcgcgatg acggcgccca ggccggcgga
    12901 gctcacgggg tgcgcgccca cggccgccag gatgaagcgc acgggcggct gatcgggctg
    12961 ttcgctggcg agctctcgct gcagcacctc cgccgtgacg gcgatgttgg tgacgatttc
    13021 ctcggtgacc cgggccggcg ttcccagagg catcgtgagc ttcgcgctga tccggtccgc
    13081 cggcagcgag ggaaaataag tgaacggcat gtgtccgccg gcgaccaggc caaccgttat
    13141 cccgagaatg gccaccgcgc cggccgcggt gacgtagcgg gcgtccgtgg ccgcgctgac
    13201 caggcgccgg aagcggaact ggatgacgtg ctcgaggcgg tcggaaaatt gcttctgcag
    13261 ggcgatcacc cgagcggcaa agcgctcgaa gcctccttgg atgctccagg cgatcagcac
    13321 cgccgtcgtg ccaatgccga tggcaaaaaa gctgcgcagg tcccaggcga actccatcag
    13381 cacgatggcc accagcggaa ccatcatcag gcccacctcg cccgcggcgg tctgcacgcg
    13441 gcggtgaccc agatgcgcgg gcagtaccag ctgggattcg atcagcgaga acgtcaggca
    13501 acaaagcacg atcacgctga tcaccgactg gatctgccct gtgggccccg gcccgaaggt
    13561 gagcggtaag aacgccgtga tcgtggtcag caccccgaag atgacgggca tggatacctg
    13621 tcgtgtgccc tcgatggccg cgctgatgag cgtttcactc gggggtgact cacccgcccg
    13681 ggactgcaga gagtgcacgc gctcgccgat caccaccgcg tcatccacca gaatgcccag
    13741 cacgagtatg aagccgaagg tggaaatgcc gtcgatggac aaaccggcca gattggcgac
    13801 gaagattgcg cccagaaagg ccgcgggtac gcccacgctg acccagaaag ccaggcgtgg
    13861 acgtaggaac aaggcgagca tcaggagcac cagcatgaat ccctgccgcg cgctgttcat
    13921 cagggtatcc agacgatctc gcatgaactc ggaattgtcg ttccagatcg tcagatccac
    13981 gccctgtggc agacgttcgt cggcatcatc caggtaggat ttcacggctc tggtcactgc
    14041 cagaatgtcc tggtcgccca ctcggaaaac ctgcacgagc gcggcgggct tattgtcgaa
    14101 ccagacccgc tcgtcggtgt cctcgaagcc gtcgaccact gtggccaggt cccgcaggaa
    14161 cacacgggta ccatccccgc gcgtgagtat caccaggtcc tcgaactctg agccccggta
    14221 tgcctgaccc tcggtgcgca gcaggacttc gccgctttcg gtcttgatgg cgccgcctgg
    14281 aaggtccaga gaggcggcgc gcacggcgtt ggcgacctcg tcgaagctca aaccgtagcg
    14341 gcgcagggtc gcctcggaaa tttcgatgga gatctcgtag ggccgcacgc cagagacgtc
    14401 cacccgggtg attcccggaa gagctgccag atcgtcgcgc acctgttgag caatggactt
    14461 gagctcccgc tcgtcgctcg ggccggtcac ggcaatctcg accacggttc tctctgccgc
    14521 gaccagcgtg atgatgggtt tctcagcctc ctgggggaac gtctcgatgg cgtcgacccg
    14581 attcttgacg tcgccgagaa ccttgttgtc atcggcgttc tcgaacagct ccacgttcac
    14641 cgtgcagcgg ccctccacgg cattggatcg gatgcgctcg atcccctgta ggccctcgat
    14701 ggcttcctcg atgcggatgc acacgccgtc ctcgacctct tcgggagcgg cacccaggta
    14761 gcgcaccgac accgtgatca gtttgtagtc caggtccggg aagtcctttt gaggcatgat
    14821 cggcagggat agcaggccgg ccagaaccat gaccgcaagc agaaggttgg ctgcgatgtg
    14881 gttgcgggca aaccaggcga tggggccgtt catgagggcg cggctcgctg ctcgacatcg
    14941 gcaacgcgaa ccagcatgcc atcgctggtg ctctgcagcg gcgagatgca gacccgctcg
    15001 ccccgggcca ggccgccgac gatgtagacc tcctcctctg ccacccggta gacgtcaacg
    15061 ggcctgaagc gcagcctctc gtcctgatcc acgacgagca cccggtcgcc tcccctcagt
    15121 gccgatcggg gcagaatgac cacgtcactg agcgttttgc cttgaatgtc cgcatcgacg
    15181 aagagcccga ccgacagagg cggccgcccg tcccggctga cgtagggctc gggaactctt
    15241 gcgactacgt gcaccatgcg ggtcgccggg tccagctcac cttccgtacg cacgacctcg
    15301 ccctgccact catgctccac gccggcgaaa cgggcacgca gggtcaccgg tatggggatc
    15361 gaagactcgt cgcccgtata cccgagcggc agatcgagaa acgcgagctc ctcgtcttga
    15421 atgggtagcc ggacctccgc gaagtccacg gcgtagatcg tcgccaccgg gttgccccgc
    15481 ttgacgaact ggccgacgtc caccctttcg ttgcgaaccc gcccctggta gggcgcgacc
    15541 aggcgggtcc tgtccaggtc gcgttccgct ctggagagcc gcgcccgcgc ttcgcgcagc
    15601 gtggcctgag caatgcgttc ccggtttgcc gcgtcgtccc gatccgagtc gctggtcgct
    15661 ccgcggcgga cgagatcctg aatgcgggtc agtgacttcc tggcgttctc cagatcactc
    15721 tctgccctgg ccaaacctgc ccgggcatgc tcgagggcca cctcgtagtc gacgggttcg
    15781 atctccagca gcacgtcgcc ctggttgaag aaaccgccgg agacgaggga cggcgagact
    15841 gccgtgacac ggccgtccac ctccggcacc agatcgcttt ccgtgcgcgg gaccaccgtg
    15901 ccgtgggtgt ggctcgtcat ctgcacctca cggggctcgg cctcgatgac ccgtacgagc
    15961 ggaggaatgg cgcttggcgc ctggggctgc attttcggcc cgctcagaaa caccagcacg
    16021 gccacggcaa agccccctag gagcaccagc gcggcctgaa gccttcgatt cattcaccgt
    16081 ctccgctggc accgtctccg ctggcaccgt ctccgctggt tcccggctgg gtgagccccg
    16141 caacgacgaa ctccacgagc tgatcgatgc ctgcttgcag ggagccttct gcggggggcg
    16201 agtctgcctg aaagaagttc aatgggtgtc tgaaccgcgc cagctggatc atcgatccga
    16261 tcatgaggtg aaagcgccag cgcaatacct ccacggggac gtcgggcagc gcggcgtgca
    16321 gggcggcgaa gtaggcctgc acaaccggcg cgaaggtctt ctcgagcagc ggcacggaga
    16381 cggatttcgg ttccgcgaac agatgggcga ggaactggtc gaggtgctcg ttgtccgtga
    16441 gcgcgaattc cagatcgacg aaggcgcgaa cgagcgcttc caccgaaggc gagctggacg
    16501 acgcctgaag ctggcgcagg cgccgcatgc gctcgtcgtt gattggcgcg atgcagcgct
    16561 cgaacgctgc atcgagcagc tgagcctttg acccgaagtg ataattggcg gcggcctggt
    16621 tcacgtcggc ggcgtcggta atggccctca gcgaagtgtt gcggtagccg cgctcgccga
    16681 aaagcttcac cgaggcttcg agaatacttt cccgggtggc tgccgagtct gcgcgcaacg
    16741 gtctggatgc ctgagccacg cggcattcct agttcaaacg gtcgtttaaa ttagcacgcg
    16801 gtgctcggga ctgtcacgcg ctgattgcga actcggctgg cacgcggggc gcggcggcgc
    16861 gacaatgccg ctcgctatct ctcaacgagc caaaaggagg gcctctcatg ctgggctacg
    16921 caaccattgg ggtgaatgac atggatcggg cggtcgcctt ctacgatgcg ctgctcgcgg
    16981 agctgggtgc aaagcagctc atgggaatgg accgcatcaa gatgtacgga accagtccgg
    17041 atgccggcat gctggccatc tgcatccctt acgatgaaga acctcaaagc cccggcaacg
    17101 gcaacatggt ggccatatcc gccggatccc gggaggccgt ggatcggctc tacgccaagg
    17161 cgatggagct cggtgctgcc gacgagggag aacccggcga gcgcaatccc gtcttctatg
    17221 gcgcctacgt cagggatctg gacggcaaca aactctgctt ctacgagatg aagctggggt
    17281 agccgccagg cggcgatccg gtcatgcgat ccgctcaggc ggcgcccgcg acggacggtg
    17341 aaagcgagcg acttcatcgg cggtaccggc aggggcgcgt gtttcctcgc gccgtggttg
    17401 ccagcgtgga catccaggcg ccggcccggg aggtctgggc agtgctggtg gattttgccc
    17461 gctatccgga atggaacccg ttcacgcctg aggtagaggc ggagctggct gtgggcgcgc
    17521 cggttcgcct gcgggtgagc atgccgggtc ggcgcgagct agagcgggtg gagcagatca
    17581 atcgggtgga accggag
//
LOCUS       DBNHKV010000339         3819 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_400833, whole genome shotgun sequence.
ACCESSION   DBNHKV010000339 DBNHKV010000000
VERSION     DBNHKV010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_400833"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="AC16PP_10815"
     CDS             <1..891
                     /locus_tag="AC16PP_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR6482162.1"
                     /translation="GSAVSHLMPGDHVVLSSDSCGVCRRCHAGLPSYCDEFVERNLTG
                     FRTDGTSPLSCRGEPLRGRFCGQSSFATAAISPGRSAVKVPQDLPLELLGPLGCGLTT
                     GVGTVMNALNPGPGSSIAVFGVGTVGLSAVMGAVLSGCEAIIAVDPNLARLEVARELG
                     ATECIDAGREDVAADIVELTRGGADFSVECSGHPAAIDQAVACLGRPGWCAQVGAPTA
                     GTRFPLDMDHVGFGRGIRGVVLGEANVHTFVPYLASLYRDGRLPYDRLVRYYDFAEID
                     RAVHDSAVTGEVIKPILRMS"
     gene            915..1964
                     /locus_tag="AC16PP_10820"
     CDS             915..1964
                     /locus_tag="AC16PP_10820"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF040257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent amine dehydrogenase family
                     protein"
                     /protein_id="HIR6482163.1"
                     /translation="MALKVFQWASGTVGRHAALAVLERDGLELVGLHTLSAAKAGRDV
                     GELLGVAATGLRATSELEAVLESDADVVVHAPLASLVYGEDPGRDVEDICRLLASGKN
                     VITVVGYMYPKVYGPELVARLEDACRAGKSTFHSTGLNPGWMGDLVPLTMSALSRTID
                     RVHVLEISNFQGYPSPEIMFESMGFGSSPEEFERSGERRRNWLNGLFRESVQMVADGL
                     GLELDDVVEEMTLEFAPRDLETAAGTVRQGTVAGQHWEWAGVLGGDKRVIHETVWRMH
                     ADVAPDWPQGRHAVTVHGVPRIHLEFEADYVSDGLLATAMHAVNAIPYVCQSEPGIRT
                     FLDLPWIMYRGAGAP"
     gene            complement(1983..3131)
                     /locus_tag="AC16PP_10825"
     CDS             complement(1983..3131)
                     /locus_tag="AC16PP_10825"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HIR6482164.1"
                     /translation="MGTSGPARALRFGSATLTVTTMYTLLITFFTLAIVFSFLCSLWE
                     AVLLSITPAYAEIQKQHGSAIGRHLSTFKSNIDRPLAAILTLNTIAHTVGAIGVGGQA
                     AAIWAETSPWVTKAIIPVAMTLAILILSEIIPKTIGATFWKELAPFTVRSLLVVIAAL
                     GPLVWLSQRVTRILKREAGDSVLSRSDFLAMTKIGERAGVFEKSESEIIKNLLRFEQV
                     CAKDIMTPRTVVIAEPESRTVAEFYAAHDQLRFSRIPIFSDAKDHITGYVLKDELLSS
                     LVQQEGDRTLGDFRRDIMVAKEDQPLPDLFSRLVEKREHIALVVDEFGGMAGIVTMED
                     VIETLLGLEIVDESDQVYDMQAHARAQWARRAKAHGLTETDAVGPTSS"
     gene            3266..>3819
                     /locus_tag="AC16PP_10830"
     CDS             3266..>3819
                     /locus_tag="AC16PP_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6482165.1"
                     /translation="MGAIEQPFEMIRYERPGEHVARIMLAREETRNAQNVQMLYEIDA
                     ALDAAMQDDDVRAVIIGADGPHFSSGHDLKGGGRIGDFGPPKMGVGGFGKPGQEGHMS
                     HEQEVFIGFCWRWRNMPKPTIAAVQGKAIAGGLMLVWPFDLIVASEDAQFSDPVVTFG
                     VNGHEYFVHAWEVGHRKAKEMLFTG"
BASE COUNT          610 a         1194 c         1317 g          698 t
ORIGIN      
        1 ggcagcgcgg tctcgcacct catgccgggc gatcacgtgg tcctcagcag tgactcatgt
       61 ggcgtctgtc ggcgctgcca cgccgggctt ccctcctact gcgacgagtt cgtggagcgc
      121 aatctcaccg gctttcgcac ggatggaacg agtccgctgt cctgccgggg cgagcctctg
      181 cgcggacgct tctgcggcca atcttcgttt gcgaccgccg ctatctcgcc tggacgcagc
      241 gcggtgaagg tgccccagga tctgccgctt gagcttctcg gcccgctcgg ctgcggtctg
      301 accaccgggg tcggcaccgt gatgaatgct ctgaacccgg gtccgggttc gagcatcgcc
      361 gtattcggcg tcggcacggt gggcctttcc gccgtcatgg gcgcggtgct ctccggctgc
      421 gaggcgatca tcgccgtgga tcccaacctg gcgcggctgg aggtggcccg cgagctcggc
      481 gccacggagt gcatcgacgc ggggcgcgag gatgtcgccg cagatatcgt agagctgacc
      541 cggggcggcg ccgatttctc cgtggagtgc tcgggtcacc ctgcggccat tgatcaggcg
      601 gtggcgtgtc tcgggcgtcc gggctggtgc gctcaggtgg gcgcgccaac cgcgggaacc
      661 cgtttccctc tggacatgga tcatgtgggg ttcggcaggg gcattcgcgg cgtggtcctc
      721 ggcgaggcca acgtgcacac tttcgtgccc tacctcgctt cgctctaccg cgacgggcgg
      781 ctgccctatg atcggctggt gcggtactac gatttcgccg agatcgaccg tgccgttcac
      841 gacagcgcgg tcaccggtga ggtgatcaag cccatactga gaatgtccta gcgcaggtat
      901 ttggagggga tcccatggcc ctgaaggttt ttcaatgggc cagcggcacc gtcgggcgtc
      961 acgctgccct ggccgtgctc gaacgtgacg gcctggagct cgttggcctg cacaccctgt
     1021 ccgctgccaa ggcgggccgg gatgtgggtg agctgctggg cgttgccgcc accggcctgc
     1081 gggcaaccag cgaactcgaa gccgtgctgg agtccgacgc cgacgtggtc gttcacgcgc
     1141 cgctggcctc gctcgtttac ggcgaggatc cgggtcgtga cgtcgaggac atctgcaggc
     1201 tgctcgcctc gggcaagaac gtcattaccg tggtgggcta catgtatccg aaggtctacg
     1261 ggccggagct ggtcgcgagg ctggaggatg cctgccgcgc gggtaagtcc accttccact
     1321 ccacgggctt gaatcccggc tggatgggag acctggtgcc gttgaccatg agcgcgctgt
     1381 cgcggacgat cgatcgcgtc cacgtcctgg agatctccaa ctttcagggt tacccgtcgc
     1441 cggagatcat gttcgagtcc atggggttcg gatcgtcacc cgaagagttc gagcggtccg
     1501 gggagcggcg ccgcaactgg ctcaacggcc tcttccggga aagcgtgcag atggtggccg
     1561 acggtcttgg gctcgagctc gacgacgtgg tggaagagat gaccctcgag tttgctcccc
     1621 gcgacctcga aaccgcagcc gggactgtcc gccagggaac cgtggccggc cagcattggg
     1681 aatgggccgg cgtgctcggg ggcgataagc gggtgattca cgaaactgtc tggcgcatgc
     1741 acgccgacgt cgcgcccgac tggcctcagg gccgccatgc cgtcaccgtg cacggcgtgc
     1801 cgcgcattca tctggagttc gaagccgact acgtcagcga cggtctgctc gccaccgcca
     1861 tgcatgcggt gaacgccatt ccctacgttt gtcagtccga gccgggaatc aggacgtttc
     1921 tggatctgcc ctggatcatg taccgtggtg ctggcgcccc atgagcctgc cgccaggcgt
     1981 cgtcaggaag acgtcggccc gacagcgtcc gtttccgtga gtccatgggc ctttgcgcgt
     2041 cgggcccact gcgcccttgc gtgggcctgc atatcgtaga cctgatcgga ctcgtcgacg
     2101 atctccaggc cgagcagcgt ctcgatcacg tcttccatgg tgacgatgcc ggccatgccg
     2161 ccgaactcgt ccaccaccag ggcaatgtgc tcgcgcttct ccacgagcct cgagaacagg
     2221 tccggcaggg gctggtcctc cttcgccacc atgatgtcgc ggcggaagtc gcccagggtc
     2281 ctgtcacctt cctgctgaac caggctggac agcagctcgt ccttgagcac gtagccggtg
     2341 atgtggtctt tcgcatcgct gaatatcggg atgcgggaaa agcgcagctg atcgtgcgcg
     2401 gcgtagaact cggccacggt gcggctttcc ggctcggcaa tcaccacggt gcgcggcgtc
     2461 atgatgtctt tggcgcaaac ctgctcgaag cgaaggagat tcttgatgat ctccgactcc
     2521 gacttctcga agacgccggc ccgctcgccg atcttggtca tggcgagaaa gtcggagcgc
     2581 gagaggacgc tatctccggc ctcccgcttg aggattcggg tgacccgctg gctcagccag
     2641 acgagcggtc ccagcgcggc gatcaccacg agcagcgagc gcaccgtgaa aggcgccagc
     2701 tccttccaga aggtggcgcc gatggtcttc gggatgatct ccgacaggat gagaatggcg
     2761 agcgtcatcg ccacgggaat gatggccttg gtgacccagg ggctggtttc cgcccagatg
     2821 gcggcggcct gaccacccac gccgatggcg ccgacggtat gggcgatggt gttgagcgtg
     2881 aggatggcgg ccagcggccg gtcgatgttg ctcttgaaag tgctcaggtg gcgcccgatt
     2941 gccgagccgt gctgtttctg aatctcagcg tacgcgggcg taatgctcag cagcacggcc
     3001 tcccacaggg agcagagaaa agagaaaacg atggccagcg tgaagaaggt gatcagcagc
     3061 gtgtacatgg tcgtgactgt gagcgttgcg gacccaaagc gcaacgccct ggccggaccg
     3121 gacgttccca tggtgccggg cggaagttgc tggctgatcg gccagggatt agagtcggca
     3181 gtcgccgtgc ccgggggcgc ggcttccggc aaacagggtc cgagccgcgg acgcgcgcgg
     3241 gcccgccttg aattgggggg atgtcatggg tgccattgag cagcctttcg agatgatccg
     3301 ctacgagcgg cccggcgagc acgtggcgcg catcatgctg gcgcgggaag agacccgcaa
     3361 cgcgcagaac gtccagatgc tctacgagat cgacgcggcg ctggatgcgg ccatgcagga
     3421 cgacgacgtg cgggcggtga tcatcggcgc cgacggcccg catttctcgt cgggccacga
     3481 cctgaagggc gggggccgca tcggcgactt cggacccccg aaaatgggcg tcggcggttt
     3541 cggcaagccc gggcaggaag gccacatgtc tcacgagcag gaggtcttca tcggcttctg
     3601 ctggcgctgg cgcaacatgc ccaagccgac catcgccgcc gtgcagggga aggccatcgc
     3661 cggagggctc atgctggtat ggcctttcga tctcatcgtc gccagcgagg atgcgcagtt
     3721 ctccgacccc gtggtcacct ttggcgtcaa cggccatgag tatttcgtgc acgcctggga
     3781 agtcgggcac cgcaaggcca aggagatgct gtttaccgg
//
LOCUS       DBNHKV010000340         8732 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_260320, whole genome shotgun sequence.
ACCESSION   DBNHKV010000340 DBNHKV010000000
VERSION     DBNHKV010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8732)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_260320"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..3269)
                     /gene="carB"
                     /locus_tag="AC16PP_10835"
     CDS             complement(36..3269)
                     /gene="carB"
                     /locus_tag="AC16PP_10835"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007243709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR6482166.1"
                     /translation="MPKRTDIDSILILGAGPIVIGQACEFDYSGAQACKALREEGFRV
                     ILVNSNPATIMTDPAMADATYVEPVEWATVARIIEQERPGALLPTMGGQTALNCALDL
                     VREGVLERFGVELIGASQDAIDKAEDRERFDKAMKRIGLETPRSAIAHSMEEAVQVQA
                     QLGFPCIIRPSFTLGGSGGGIAYNAEEFAEICARGLDLSPTNELLIDESLLGWKEFEM
                     EVVRDCVDNCIIVCSIENLDPMGVHTGDSITVAPAQTLTDKEYQLLRNASIAVLREIG
                     VDTGGSNVQFAIHPETGRLVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     LDNDITGVTPASFEPSIDYVVTKVPRFTFEKFDQADSRLTTQMKSVGEVMAIGRSFQE
                     SLQKALRGLETGMTGLDPLLEIGGEEAVARLRRELATPGAERIWYVADAFRFGLSLEE
                     VFELSAIDPWFLAEIEDLIAAESQLAGKRLDQIGADALYRLKRQGFSDARLGTLLGAP
                     EQQVREARHSMGIRPVYRRVDTCAAEFPSATAYMYSTYEEECEADPSERPKIMVLGGG
                     PNRIGQGIEFDYCCVHAALAMREDGFETIMVNCNPETVSTDYDTSDRLYFEPVTLEDV
                     LAIVDVEKPKGVIVQFGGQTPLKLVEALEQAGVPIIGTSAEAIDRAEDRQRFQALIRK
                     LGLKQPSNATAVDVETGLKGAREIGFPIVVRPSYVLGGRAMEIVFNEDELARYLLQAV
                     TVSNDSPVLLDRFLDQAVEVDVDAVSDGKQVVIGAIMQHIEQAGVHSGDSACALPPYQ
                     LSADLQDEIREQVRAMALELGVIGLMNVQLAVQEGEIFILEVNPRASRTVPFVSKCIG
                     VSLAKIAARCMAGKTLAEQGFTREIIPEQVNVKESVFPFTKFPGVDPILGPEMKSTGE
                     VMGVGDSFGEAFAKASLGAGVALPSGGRAFISVKDSDKPGLPALGRQLVDLGFQLVAT
                     RGTAKCFAEAGIPSEVINKVGQGRPHVADMLKNEQIDLIINTTEGHQSIRDSAVIRRL
                     ALQNKVCYTTTLTGGEAFCIAIRYSRDHQGETVRRLQDLHADMVGAS"
     gene            complement(3262..4389)
                     /gene="carA"
                     /locus_tag="AC16PP_10840"
     CDS             complement(3262..4389)
                     /gene="carA"
                     /locus_tag="AC16PP_10840"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR6482167.1"
                     /translation="MVRALLALEDGSVFKGLSIGVEGTSVGEVVFNTAMTGYQEILTD
                     PSYARQIITFTYPQIGNTGINPEDVESDRIWASGMVIRQTPKRASNWRMRQRLEDYLV
                     AENVVAIADVDTRRLTRLIREKGAMAGCIAAGEAVSEAEAVALARDFPGLAGMDLAQV
                     VTRAEAGSWTQTDWSLESGYGEQTDARFHVVAYDFGVKRNILRLLAARGCRLTVVPAT
                     TPASSVLALEPDGVFLSNGPGDPEPCGYAIEAIGELLDRQIPIFGICLGHQLLGLASG
                     ATTVKMPHGHHGANHPVKDLRDGRVVITSQNHGFAVDADSLPDCLTATHVSLFDGTLQ
                     GIRRSDRPAFGFQGHPEASPGPQDCQYLFDEFIELMESRHA"
     gene            complement(4465..5280)
                     /gene="dapB"
                     /locus_tag="AC16PP_10845"
     CDS             complement(4465..5280)
                     /gene="dapB"
                     /locus_tag="AC16PP_10845"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR6482168.1"
                     /translation="MTRVAVTGAAGRMGKTLIQAIAEAEDLSLGAALERHDSTLLEAD
                     AGELAGVGRAGVAVGASLEDAAEAFDVLIDFSVPAATLAALEVCRERGRGMVIGTTGF
                     DADGLGRIQAAAETIPIMMAPNMSVGVNLCFKLMEIAASVLGDDVDIEVIEAHHRDKI
                     DAPSGTAVRMGEIIAVSLGRDLDEVAVYGRQGITGPRARSTIGFETIRAGDIVGEHTV
                     LFAGNGERIEITHRAQSRMNFAQGALRGVRFLAGRTSGLFDMQDVLGFKGALG"
     gene            complement(5335..6462)
                     /gene="dnaJ"
                     /locus_tag="AC16PP_10850"
     CDS             complement(5335..6462)
                     /gene="dnaJ"
                     /locus_tag="AC16PP_10850"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR6482169.1"
                     /translation="MAKRDYYEVLGVGRDASEDDIKKAYRRLAMKFHPDRNPDDASAE
                     EKFKEASEAYEVLADAEKREAYNRFGHAGVEASVGGTGGFDGGSFSDIFSDVFGDIFG
                     GGARGRSSVQRGSDLRYGLDLTLEQAVAGDNVEIRVPVLSACEVCDGSGARPGTSAST
                     CPDCGGAGQIRVAQGFFSLQQTCPRCRGAGRVIQDPCGNCGGSGREEKRKTLSVRIPA
                     GVDTGDRIRLAGEGEAGFNGGPPGDLYVQVEVRDHPIFTREGKNLYCEVPISFVDAAL
                     GGELEVPTLTGRVKLKIPPETQTGKLFRLRGKGVTPVRGGSVGDLLCRVQVETPVRLS
                     DEQKRLLQDFKATLAKGGSQHSPKETSWFQGVKDFFEGLKP"
     gene            complement(6543..8468)
                     /gene="dnaK"
                     /locus_tag="AC16PP_10855"
     CDS             complement(6543..8468)
                     /gene="dnaK"
                     /locus_tag="AC16PP_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR6482170.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGNDAKVIENAEGDRTTPSIVAYTD
                     DGEILVGQSAKRQAVTNPTNTLFAVKRLIGRKFKDDVVQKDIKMVPYKISEAPNGDAW
                     IDVKGEQLAPPQVSAETLKKMKKTAEEYLGEPVTEAVITVPAYFDDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAYGMDKKRGDTKIVVYDLGGGTFDVSIIEIAEIDGEHQFEV
                     LSTNGDTFLGGEDFDLRVIDYLADEFQKDNGVDLHRDPLALQRLKEAAEKAKIELSSS
                     QQTEINLPYITADQSGPKHLVLKLTRAKLESLVEELVTRTIDPCRTALKDANLNASDI
                     DDVILVGGQTRMPLVQQKVKDFFGKEPRKDVNPDEAVAIGAAIQAAVLSGDVTDVLLL
                     DVTPLSLGIETLGGVMSTLIEKNTTIPTKKQQVFSTADDNQTAVTIHVLQGERKQASS
                     NKSLGRFDLTDIPPAPRGLPQIEVSFDIDANGILNVSAKDKATGKEQSIIIKASSGLS
                     DDEIDQMVRDAEAHSEEDAKFEEMVTLRNQADGLIHAARKALTDAGDKASDTEKEAIG
                     SAADALEESLKNGDKADIEAKMQALSEASANLAQKMYEEQAAEAQSGAAEAGDDGAAD
                     GDTVDAEFEEVNEKDDK"
     gene            complement(8603..>8732)
                     /locus_tag="AC16PP_10860"
     CDS             complement(8603..>8732)
                     /locus_tag="AC16PP_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR6482171.1"
                     /translation="MVPNPDTEPNSVIEVMQKGYTLNGRLVRAAKVVVSAALSQDG"
BASE COUNT         1465 a         2906 c         2695 g         1666 t
ORIGIN      
        1 acaatcccgt ttgtgcagcg tgtctgagcg tcgggctagg atgccccgac catgtcggcg
       61 tgcaggtcct gcaacctgcg aaccgtctcc ccctgatgat ctctcgaata gcggatggcg
      121 atgcagaaag cctcgccgcc cgtgagggta gtggtgtagc acactttgtt ctgcagtgcg
      181 agccgacgaa tgaccgccga gtcgcgaatg gactggtgcc cctccgtggt attgatgatc
      241 aggtcgatct gctcgttctt gagcatgtcg gccacgtgag ggcggccctg gccgacctta
      301 ttgatgacct ccgagggaat gccggcttcg gcgaagcact tcgccgttcc gcgggtagcc
      361 accagctgga acccgagatc caccagctgg cggccgagcg ccggcaagcc gggcttgtcg
      421 gaatccttga cgctgatgaa agcccgcccg cccgagggca gggcgacgcc ggcacctaag
      481 gaggccttgg cgaacgcctc accgaagctg tcgccaacgc ccatgacctc gccggtggat
      541 ttcatctccg ggccgagaat cgggtccacc cccgggaact tcgtaaacgg gaagacggat
      601 tccttgacgt tcacctgttc cggaatgatc tcccgggtga agccctgttc cgccagcgtt
      661 tttcccgcca tgcagcgcgc cgcgatcttg gcaagagaaa ccccgatgca cttggagacg
      721 aaggggaccg tgcgcgaagc ccgcgggttg acctcgagaa tgaagatctc gccttcctgg
      781 acggccagct gcacgttcat cagcccgatg acgcccagct ccagggccat ggcccgcacc
      841 tgctcgcgga tctcatcctg caggtcggcg gaaagctggt acggcggcag cgcgcaggcg
      901 gagtcgccgc tatgcacgcc cgcctgctcg atgtgctgca tgatcgcgcc gatgacgacc
      961 tgcttcccgt cggagacggc atccacgtcc acctccacgg cctgatccag aaacctgtcc
     1021 agcagaacgg gggaatcgtt ggacacggtg acggcctgga gcaggtaacg ggccaactcg
     1081 tcctcgttga agacgatttc catggcccgg cctcccagca cgtaggacgg ccgcacgacg
     1141 atggggaacc cgatttcccg ggcgcccttg agccccgtct cgacgtccac cgccgtggcg
     1201 ttggacggct gcttgaggcc gagcttgcgg atcagcgcct ggaagcgctg gcgatcctcg
     1261 gcacggtcga tggcttccgc gctcgtgccg atgatgggaa cgcccgcctg ctcaagggcc
     1321 tccaccagct tcagcggcgt ctggccgccg aactgaacga tcacgccctt gggcttctcg
     1381 acatcaacga tggccagcac gtcctccagg gtcacgggct cgaagtacag ccggtccgac
     1441 gtgtcgtagt ccgtggagac ggtctcggga ttgcaattga ccatgatggt ctcgaagccg
     1501 tcctcgcgca tggcgagtgc tgcgtgaacg cagcagtagt cgaactcgat cccctggccg
     1561 attcggttcg gtcctccgcc gagcaccatg attttcggtc gctcggacgg gtccgcctcg
     1621 cactcttcct cgtaggtcga atacatgtag gcggtggccg acgggaactc ggccgcgcag
     1681 gtatcgacgc gccgatagac gggtctgatg cccatgctat gccgcgcctc acgaacctgc
     1741 tgctccggag cccccagcag cgttcccagc cgcgcgtccg aaaacccctg gcgtttgagc
     1801 cgatacagcg cgtccgcgcc gatctgatcg agccgcttgc ccgcaagctg gctttccgcg
     1861 gcgatgaggt cctcgatctc ggcgagaaac cagggatcga tggccgacag ctcgaacacc
     1921 tcctccagcg agagcccgaa acggaatgcg tcggcgacgt accagatccg ttctgcaccc
     1981 ggggtggcca gctcgcgccg caaccgggcg acggcttcct caccgccgat ttcgaggagc
     2041 gggtcgagcc cggtcatccc ggtctccaaa ccccgcagcg ccttctgcag ggactcctga
     2101 aagctgcggc cgatggccat gacctcaccg acggacttca tctgcgtcgt gagccgtgag
     2161 tcagcctggt cgaacttctc gaaggtgaaa cggggcacct tggtgacgac gtaatcgatg
     2221 cttggctcga aagaggcggg ggtcacgccc gtgatgtcat tgtcgagctc gtccagcgtg
     2281 tagcccactg ccagcttcgc ggcgatcttg gcgatgggga agccggtggc cttggaagcc
     2341 agcgccgacg accgcgaaac cctcgggttc atctcgatca ccaccaaccg accggtttcc
     2401 gggtggatgg cgaactgcac gttggagccg ccggtatcca ccccgatttc ccgcagaacg
     2461 gcgatggagg cgttgcgcag cagctgatat tccttgtccg tgagcgtctg cgccggagcc
     2521 accgtaatgc tgtccccggt gtgcacgccc atggggtcca gattttcgat tgagcagacg
     2581 atgatgcagt tgtccacgca atcgcggacc acctccatct cgaactcctt ccagcccagc
     2641 aggctctcgt cgatgagcag ctcgttggtg ggcgacaggt cgagcccccg ggcgcagatc
     2701 tccgcgaact cctccgcgtt gtaggcgatg ccgccgccgc tgccgcccaa agtgaacgac
     2761 gggcggatga tgcacggaaa accgagctgg gcctggacct gaacggcttc ctccatgctg
     2821 tgcgcgatgg cagatcgggg tgtctccagg ccaatgcgct tcatggcctt gtcgaacctt
     2881 tcccgatcct cggccttgtc gatggcgtcc tggctggcac cgatcagctc gacgccgaaa
     2941 cgctcgagca cgccttccct gaccaggtcc agcgcgcaat tgagcgccgt ctgcccgccc
     3001 atggtcggca gcagcgcacc cggcctctcc tgctcgatga tgcgcgccac ggtggcccac
     3061 tccaccggct cgacgtacgt cgcgtcggcc atggcgggat ccgtcatgat ggtggcggga
     3121 ttggagttga cgagaatcac ccggaagcct tcctcgcgca gcgctttgca ggcctgagcg
     3181 ccggagtagt cgaactcgca ggcctggccg atcacgatgg gccccgcgcc gagaatgagg
     3241 atggaatcga tatcggtacg cttaggcatg gcgcgattcc atgagctcga tgaactcgtc
     3301 gaacagatac tggcaatcct ggggcccggg gcttgcctcc ggatgcccct ggaaaccgaa
     3361 ggccggccgg tcggagcgac gaatgccctg cagcgtcccg tcgaatagag agacatgggt
     3421 ggccgtgagg cagtcgggca ggctgtcggc atccaccgcg aaaccgtgat tctggctggt
     3481 gatgacgacg cggccatcgc ggaggtcctt gaccgggtga ttggccccgt ggtgaccgtg
     3541 gggcatcttg acggtagtgg ccccgctggc caggcccagc agctgatgac ccaggcagat
     3601 accgaatatg ggtatctggc gatcgagcag ctcgccgatg gcctcgatgg cgtaaccgca
     3661 cggctccgga tcgccgggac cgttggatag aaagaccccg tccggctcca gggcaagaac
     3721 gctggaggcc ggggtggtgg ccggcacgac ggtcaggcgg cagccgcgcg ccgccagcag
     3781 gcggagaatg ttccgcttga cgccgaagtc gtaggcgacg acgtgaaaac gcgcgtccgt
     3841 ctgctcaccg taaccgcttt cgagcgacca gtcggtctga gtccaggaac cggcctcggc
     3901 cctggtcacg acctgcgcca ggtccatgcc agcaagcccg ggaaagtctc gggccagcgc
     3961 cacggcctcg gcttcgctga ccgcctcgcc ggccgctatg catcccgcca tggcgccctt
     4021 ctcccggatg agccgggtca gcctgcgggt gtccacgtcc gcgatggcga ccacgttctc
     4081 cgcgaccagg tagtcttcca gacgctgccg catgcgccag ttgctggccc gcttcggcgt
     4141 ctgccgaatg accatgccgc tggcccagat ccgatcggac tccacgtcct ccggattgat
     4201 cccggtgtta ccgatctgcg ggtaggtgaa ggtgatgatc tggcgggcgt acgacggatc
     4261 ggtgaggatt tcctggtagc ccgtcatggc ggtattgaaa acgacttccc cgaccgatgt
     4321 accttctaca ccaatcgaca gacctttaaa cacactcccg tcctcgagag cgagcagtgc
     4381 ccgaaccaaa tcgttttcca ataacaggtc gcaaaaaggc gagatcgccc agaaccgatc
     4441 ccgccttgaa ttcggggcgg tagtctaccc aagtgcaccc ttgaagccca gcacatcctg
     4501 catgtcgaag aggcctgagg ttcggcctgc aagaaaccgg acgccccgca gcgcgccctg
     4561 ggcaaagttc atgcggctct gggcgcggtg ggtgatctcg attcgctctc cgttgcctgc
     4621 aaaaagaacc gtgtgctcgc ccacgatgtc gccggcccgg atggtctcga aaccgatggt
     4681 gctgcgcgcc ctggggcccg tgatcccctg gcgtccgtac acggctacct cgtcgagatc
     4741 gcgccccagc gacacggcga tgatctcccc catcctgacg gccgtgccgg agggcgcgtc
     4801 gatcttgtcg cggtggtgcg cctcgatcac ctcgatgtcc acatcgtcac ccagcaccga
     4861 ggcggcgatc tccatgagct tgaagcacag attcaccccc acgctcatgt tgggcgccat
     4921 catgatcggg atcgtctccg cggcggcctg aatccggcca agcccgtcgg catcgaatcc
     4981 cgtggtgccg atcaccatgc cgcggccgcg ctcacgacag acctccagcg ccgccagggt
     5041 ggctgccgga acgctgaagt cgatgagcac gtcgaaggct tcggcggcat cttcaagcga
     5101 agcccccacg gcgacgcccg cccggcccac accagccagt tctcctgcat cggcttctaa
     5161 aagggtggag tcatgtcgtt ccagcgcagc gccaaggctc aggtcttccg cctcggcgat
     5221 ggcctggatc agtgtcttgc ccatccggcc cgccgcaccc gtcacggcaa cacgcgtcat
     5281 cttccggctc cctagttgct ccggccggta tagtaggcga tatctaggga cggatcaggg
     5341 cttcaagccc tcgaaaaaat cctttacgcc ctgaaaccac gaagtctctt tgggcgaatg
     5401 ctgggagccc ccctttgcca gggtggcttt gaagtcctgc agaagccgct tctgctcatc
     5461 ggagaggcgg acgggcgtct ctacctgcac acgacagagc aggtcgccca ccgatccgcc
     5521 ccggaccggg gtcacgccct tgccgcgcag ccggaacagc ttgccggtct gggtttcggg
     5581 cggaattttg agcttgaccc ggccggtgag cgtcggcacc tccagttctc cgccgagggc
     5641 cgcgtcgacg aagctgatcg gcacctcgca gtacaggttt ttgccctcgc gggtgaagat
     5701 cgggtggtcg cggacctcga cctgcacgta caggtcgcca ggcgggccgc cgttgaagcc
     5761 cgcctcaccc tcgcccgcca gccggatcct gtcaccggta tcgaccccgg cggggatccg
     5821 taccgagagc gtcttgcgct tctcctcccg gccactgcca ccgcagttgc cacagggatc
     5881 ctgaatcaca cgcccggcac cgcggcatcg agggcacgtc tgctggaggg agaagaatcc
     5941 ctgggccacc cgaatctggc ccgcaccgcc acagtccggg caggtgctgg cgctggttcc
     6001 cggccgcgcg ccggagccgt cgcagacctc gcacgccgag agcacgggca ccctgatctc
     6061 gacgttgtcg ccggcgaccg cctgttccaa cgtgagatcg aggccgtagc ggaggtctga
     6121 accacgctgc accgagctgc ggccgcgggc gccgccaccg aagatatcgc cgaagacgtc
     6181 gctgaagatg tcgctgaagc tgccgccgtc gaacccgccc gtaccgccga cactggcttc
     6241 gacgcccgca tgaccgaagc ggttgtaggc ctcgcgcttc tcagcatccg cgagcacctc
     6301 gtaggcctcg ctggcctcct tgaacttctc ttccgcggag gcgtcgtcag ggttccggtc
     6361 cgggtgaaac ttcatggcga gtcggcggta ggccttcttg atgtcgtctt ccgacgcatc
     6421 acggccgacc cccagaacct cgtagtaatc ccgtttagcc atctcgctca cacaaaaagg
     6481 cgcacgtctt tcgcgtgcgc ccccaaccgc cgaaagcaga tcataatgcc tgcgaccaac
     6541 gtttacttgt cgtccttctc attcacctct tcgaactcgg cgtctaccgt gtcgccatcc
     6601 gccgccccgt cgtcgcccgc ctcggcagca cccgactgag cctccgcggc ctgctcctcg
     6661 tacatcttct gggcgaggtt ggcggacgcc tccgagagcg cctgcatctt ggcttcgatg
     6721 tcggccttgt cgccgttctt cagagactcc tccagggcgt ccgccgcgga tccgatggcc
     6781 tccttctccg tatccgaggc cttgtccccg gcatcggtta gggccttgcg ggcagcgtgg
     6841 atgagcccgt ccgcctgatt gcgcagggtg accatctcct cgaacttggc gtcctcctcg
     6901 ctgtgcgcct ccgcatcgcg gaccatctgg tcgatctcgt catcggaaag gccgctggag
     6961 gccttgatga tgatggactg ctccttgccc gtcgccttgt ccttggccga cacgttcagt
     7021 atgccgttgg cgtcgatgtc gaagctcacc tcgatctgag gcaggccgcg tggcgcgggc
     7081 gggatgtcgg tgaggtcgaa acggccgagc gacttgttgg agctcgcctg cttgcgctct
     7141 ccctgcagga cgtgaatggt cacggccgtc tggttgtcgt cggcggtgga gaacacctgc
     7201 tgcttcttcg tgggaatcgt ggtgtttttt tcgatgaggg tgctcatcac cccgccgagg
     7261 gtctcgatac ccagcgacag aggggtcacg tcgagcagca gaacgtctgt gacgtcgccg
     7321 gagagaacgg cggcctggat ggccgcgccg atggcgaccg cctcgtccgg attgacgtcc
     7381 ttgcgcggct ccttaccgaa gaagtccttg accttctgct ggaccagcgg catgcgggtc
     7441 tggccgccca ccaggatgac gtcgtcgata tccgaggcgt tcaggttcgc gtccttcagc
     7501 gccgtgcggc agggatcgat ggttcgagtc accagctcct ctaccaggga ttcaagcttg
     7561 gcacgcgtca gctttaggac cagatgcttg ggaccggact gatcagccgt gatgtaaggg
     7621 aggttgatct cggtctgctg gctcgaggag agctcgatct tggccttttc cgccgcctct
     7681 ttcaggcgct gcagcgcgag cggatctcta tgcagatcca cgccgttgtc cttctggaac
     7741 tcgtcagcca ggtaatcgat cacacgcagg tcgaagtcct cgccacccag aaaggtatcg
     7801 ccgtttgtgg agagcacttc aaactgatgc tcgccgtcga tctcggcaat ctcgatgatg
     7861 gacacgtcga aagttccgcc gccgaggtcg tagaccacga tcttggtgtc gccccgcttt
     7921 ttgtccatgc cataggcaag cgccgccgcg gtgggctcgt tgatgattcg cttcacgtcc
     7981 agaccggcta tgcggccagc gtcctttgtg gcctggcgct gtgagtcgtc gaagtaggcg
     8041 ggcacggtga tcaccgcttc cgtgaccggc tccccgaggt actcctcggc cgtcttcttc
     8101 atcttcttga gcgtctcggc ggacacctgg ggcggtgcga gctgctcgcc cttgacgtcg
     8161 atccaggcgt caccgttcgg cgcttctgag atcttgtagg gcaccatctt gatgtccttc
     8221 tggaccacgt cgtccttgaa cttgcgcccg atgagccgct tgacggcaaa cagcgtgttg
     8281 gtggggttcg ttaccgcctg gcgctttgcg ctctggccaa cgaggatctc cccatcatcg
     8341 gtataggcga cgatggacgg cgtggtacgg tcgccctcgg cattttcgat gaccttggca
     8401 tcgttgcctt ccagcaccgc cacgcatgag ttggtggtac ccaagtcgat accgatgatc
     8461 ttacccattc ttcatttctc cgatgtgtcg gtcccgttga tccggctcga agccgttcac
     8521 gtgaccgtga attcaattct tcccgctata tttgtccgat tggacggact tcaacccctg
     8581 ggtcgagtac gggcgcaatc cgtcaaccgt cctgactcag ggccgcgctc acaaccactt
     8641 tggcggcccg aaccagcctg ccgttgaggg tgtagccctt ctgcatcacc tcgattaccg
     8701 agttcggctc cgtatccgga ttcgggacca tg
//
LOCUS       DBNHKV010000341         8068 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1960183, whole genome shotgun sequence.
ACCESSION   DBNHKV010000341 DBNHKV010000000
VERSION     DBNHKV010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1960183"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="AC16PP_10865"
     CDS             <1..315
                     /locus_tag="AC16PP_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6482172.1"
                     /translation="QHRLVAFDDRARMFSYELTAGKPIGMQDYVVVATVTPIDDDHCT
                     IRWAGRMTADASLNEAETGRALEVALGNMTSGIIARLKGEEPAFARQPNEDWQLTSDR
                     GP"
     gene            416..2041
                     /locus_tag="AC16PP_10870"
     CDS             416..2041
                     /locus_tag="AC16PP_10870"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6482173.1"
                     /translation="MTSQNQPDYDVIGIGAGFAGLALIHYMREAGLSVRVFDKASDIG
                     GTWTWNRYPGAASDSECYYYCLTFSKELLQEWQWSVRYPGWEENLSYMHFVADKCDMW
                     PHIQLNTHVASADYQEDTGHWLITTADGDQYTCKYFVSAMGMISEPVIPNVKGMDTFK
                     GPCFHSARWPEEGLDYAGKRVGIIGAGATTVQMLPVMAETAASVTVFQRTPNFIMPAV
                     QKTMTPDWEKEIKEHYDEIIAKCRNHVFGMAFDSPVGRNLADTPPDEVQRIFEEHWPR
                     GSFRFVFETFDDLLTNPESNRIAGEFVISKMKEKVKDPEVAETLTPKGYPFFAKRPPL
                     DHGYYEAYNRDNVKLVDIKDREPIVEITETGIRTTENEYELDIIVLATGFQAYTGAQE
                     ALAIRGRNGQLLRDKWEDVSASIMGVFVADFPNMFLITGPQAPFANLPTSIEQNVLYI
                     VDCIKKMESEGFDVCEPQKQAEDEWVEHTAEIHAQTLMAQGDKVHSWMMGANLEDRKP
                     RVLIYFGGANVYYDKMRESADAGFPEVEFERIA"
     gene            2055..2873
                     /locus_tag="AC16PP_10875"
     CDS             2055..2873
                     /locus_tag="AC16PP_10875"
                     /inference="COORDINATES: protein motif:HMM:NF016658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HIR6482174.1"
                     /translation="MRSPTALVWALILALAAQNLCAAPKAQLWEFWTASDSKSTLQVD
                     HGAWQEFLDRYLDPGADGINRIRYARVTAEDRALLQGYLGVLGRTDPRELHRTEQLAY
                     WINLYNALTVEVVLRHPEKDSILRMGKGLFSMGPWNEKLIELAGEPVTLNDVEHRILR
                     PIWRDHRIHYVLNCASLGCPNLSGDVYTRSSLEDQLREAETAYINHERAVTFNDGGGL
                     RLSSIYKWYRDDFAPDSKGLLSYLADHHQSLADRLRAHDRDVEFDYDWSLNRAD"
     gene            complement(2957..3970)
                     /locus_tag="AC16PP_10880"
     CDS             complement(2957..3970)
                     /locus_tag="AC16PP_10880"
                     /inference="COORDINATES: protein motif:HMM:NF014615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR6482175.1"
                     /translation="MTASRQTPAKLDPGSEVRAKRSHEMAPGGRYSAMESRLQDGEIV
                     LLDGGTGTELEKRGAVMDPAAWCGPATLDNAEVLKDVHLDYISSGADIITTNTYASSR
                     LMLEPAGCGDRFEEINRAAVTAAHEARDLSGRADVLVAGSLSHMCPIAAGTDRADADR
                     EPPVEVMREAFDELAMLLRDTGCDLILLEMMYCPRRVPLAFEAALATGLPVWAGFSAR
                     RDERGEVVGFGRDACVPLADILSVLEWGEVEAAGLMHTAADVVADAISVLRERYPGML
                     MAYPDSGYFRMPNWQFDDIIEPEALVGFARGWVDNGVQILGGCCGLSPDHIAALHHAF
                     RGR"
     gene            complement(3967..4383)
                     /locus_tag="AC16PP_10885"
     CDS             complement(3967..4383)
                     /locus_tag="AC16PP_10885"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6482176.1"
                     /translation="MSRNLDQARLFFDACETGKGWEGCKEYCTPRATFSCQSGALADI
                     DSLEGYCEWMKNLLIPIPDGRYELKFFAEDEERACVAAFAVFHGTNTGPGGPGEPTGR
                     KAAADYVYAMDFDGDRIRHMTKIWNDGITLQELGWA"
     gene            complement(4491..5201)
                     /locus_tag="AC16PP_10890"
     CDS             complement(4491..5201)
                     /locus_tag="AC16PP_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482177.1"
                     /translation="MSEANASSPSTRVGTITCRCGEVTIALPNPVPKFRCGCCCTDCL
                     QRAYIGANGEPTAAIKNLEEAVDLLYVDSQIMRPGRETLARLSVFRLNKEDALNINLR
                     AECCGSVLCTENEKFHVPHSMATFNNLRPSVYCDFAAVPEPNCYVWTGDWPEERAEAL
                     ARREKSTKGEALPQIVDVMAALTEKPIIEIASALQLPAPTKAAGAISFAELCAGMEVK
                     IEHAYFQDSRAHKVARKA"
     gene            complement(5323..5832)
                     /locus_tag="AC16PP_10895"
     CDS             complement(5323..5832)
                     /locus_tag="AC16PP_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HIR6482178.1"
                     /translation="MQTPMENAMARMLATMAVGLGLWFATAGFTAAEEGAAMHLDVYK
                     SPTCGCCVKWMDHVAESGYTSTGHHPQDLGELKRELGVPARYGSCHTGVSQDGYVFEG
                     HVPARFITQFLADPPAGALGLTVPAMPVGSPGMEYKDQFMPYEVLLLNKDGSVEVFAS
                     VESYEQQFE"
     gene            6002..6397
                     /locus_tag="AC16PP_10900"
     CDS             6002..6397
                     /locus_tag="AC16PP_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482179.1"
                     /translation="MSDVASLWLLFGLPPAAPIVAALIAFAARDTGRWRWPLALSYVA
                     LVFCGAIATTNMAYHVREGYKNLPDVNWAVWAGLVWAGALTLPSAIALALRYKSMATA
                     LLTGLGVGVLSAITLISTSFGLMMMLSFE"
     gene            6584..7330
                     /locus_tag="AC16PP_10905"
     CDS             6584..7330
                     /locus_tag="AC16PP_10905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6482180.1"
                     /translation="MATCILFLAAVLLIGAAPGVQADQSFYTSKPASRDGIGKLYMGR
                     EIAHVMGHLGAGWLERPEREREERTDLLVARLPVEPDFVVADIGAGTGYFSFPIAERL
                     TDGEVLAVDIQPQMLEMIERQKAKRNIDNVSTVLGNEMDPGLPESGVDLIFIVDAYHE
                     FSHPREMGEAMFAALVPGGYLVLIEYRAEDASVPIKRLHKMSQAQTKKEMRAIGLEWL
                     RTEDYLPQQHVLVFQKPAVGSLAEPPVTAP"
     gene            complement(7317..7478)
                     /locus_tag="AC16PP_10910"
     CDS             complement(7317..7478)
                     /locus_tag="AC16PP_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482181.1"
                     /translation="MLNPQLGGVQSSEDDAAVLKADRMGQVIADRSAAAVDFDDDDRV
                     RMKLTLKAQ"
     gene            complement(7533..8042)
                     /locus_tag="AC16PP_10915"
     CDS             complement(7533..8042)
                     /locus_tag="AC16PP_10915"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIR6482182.1"
                     /translation="MRTIADFPIPGIQFRDVTTLFGDAEGFRRAVDELAEPYEGDGID
                     AVVAMDARGFILGGAVAYKLGLPFVPIRKKGKLPGKTIVEAYSLEYGDAVVEIHDDAL
                     HAGARVLLLDDLLATGGTAVAGARLIERLGGHIVAASFVVNLPDLGGQKRLEEMGLEV
                     HFLCEFEGD"
BASE COUNT         1640 a         2557 c         2409 g         1462 t
ORIGIN      
        1 cagcaccggc tggtggcctt cgatgatcgg gcccggatgt tttcctacga gctgacggcc
       61 ggcaaaccaa tcggcatgca ggactacgtg gtcgtagcca cggtgacccc gatcgatgac
      121 gaccactgca ccattcgctg ggccggcagg atgacggccg acgcgagcct gaacgaggcc
      181 gaaaccggcc gcgccctgga ggtcgccctg ggaaacatga catccggcat catcgcgcgg
      241 ctcaagggcg aagagccagc cttcgcccgg cagcccaacg aggattggca attgacgagc
      301 gatcgcgggc cttaattccg gaaggccacc cagccagcca aaggcgtcgc tgccccgagc
      361 ttggcctacc tgtggattga cgtataagct atcgatattg agataggaga ccgcgatgac
      421 atcgcagaac caacccgact acgacgtgat tggaatcggc gccggcttcg ccgggctggc
      481 tctgatccac tacatgcgcg aggccggctt gtcggttcgg gtgtttgaca aggcatctga
      541 tatcggcggc acgtggacct ggaaccgcta tccgggcgct gcgagcgaca gcgagtgcta
      601 ctactactgc cttaccttct cgaaagaact ccttcaggag tggcagtggt cggtacgcta
      661 cccgggctgg gaggaaaatc tcagctacat gcacttcgtt gcagacaagt gcgacatgtg
      721 gccgcacatc cagctgaata cgcacgttgc cagcgcggac taccaggagg acaccggtca
      781 ctggttgatc acgactgccg acggagatca gtacacctgc aagtacttcg tcagcgcgat
      841 gggcatgatt tccgagccgg tcataccgaa cgtcaaaggc atggatactt tcaagggccc
      901 gtgcttccac tccgcccgct ggcccgagga aggtctggac tacgccggta agcgggtcgg
      961 catcatcggg gcgggcgcga ccaccgttca gatgcttccg gtgatggccg agaccgcggc
     1021 cagcgtgacc gtcttccagc gtactccgaa tttcatcatg ccggccgttc agaagaccat
     1081 gacgccggac tgggaaaagg aaatcaaaga gcactacgac gagatcatcg ccaaatgccg
     1141 caaccacgtc ttcggcatgg ctttcgacag cccggtaggt cgaaacctgg ccgatacgcc
     1201 gccggacgag gtccagagaa tattcgagga gcattggcca cgcggcagct tccggttcgt
     1261 tttcgagacc ttcgacgacc tgctcaccaa cccggaatcc aaccgcatcg ccggagagtt
     1321 cgtcatcagc aagatgaagg aaaaggtgaa agatcccgag gttgccgaaa cgcttacgcc
     1381 gaaggggtac cccttctttg ccaagcggcc accgctggat cacggatact acgaggcgta
     1441 caaccgcgac aacgtcaagc tggtcgatat caaggatcgg gaacccatcg tcgagatcac
     1501 cgagaccggg atccgcacca ccgaaaacga gtacgaactc gacatcatcg tgctggcaac
     1561 cgggttccag gcatataccg gggcccagga agcgctggca attcggggcc gcaacggcca
     1621 gctgctcaga gacaagtggg aagacgtgtc tgcctccatc atgggcgtgt tcgtggccga
     1681 tttcccgaac atgttcctga tcaccggacc tcaggcgccc ttcgcaaacc tgcccacctc
     1741 catcgagcag aacgtcctgt acatcgtcga ctgcatcaaa aagatggaga gcgaagggtt
     1801 cgatgtctgt gaaccccaga agcaggcgga ggacgagtgg gtcgagcata cggccgagat
     1861 tcacgcgcag accctcatgg cacagggcga caaggtgcac tcatggatga tgggcgccaa
     1921 cctggaagac agaaaaccgc gcgtgctgat ctacttcggc ggcgccaacg tgtactacga
     1981 caagatgcgt gaatccgccg atgccggatt tccggaagtg gagttcgagc gaatagcctg
     2041 atgcggatcc gggcgtgcgc tcgccaaccg cattagtctg ggcgctgatt ctggcgctgg
     2101 ccgcgcagaa cctttgcgcg gccccgaaag cccagctctg ggagttctgg acggcaagcg
     2161 actcgaagag cacccttcag gtggatcacg gggcctggca ggaatttctc gaccgctatc
     2221 tcgatcctgg ggccgacggt atcaaccgga ttcgctatgc gcgggttacc gcagaggacc
     2281 gcgccctgct gcaaggctac ctcggcgtcc tcggcagaac ggaccccagg gagctccaca
     2341 ggacggagca gctggcctac tggatcaatc tgtacaacgc gctcaccgta gaggtggtgc
     2401 tcaggcatcc ggagaaagac agcatcctgc ggatgggaaa gggactgttc agcatgggtc
     2461 cctggaacga gaagctgatc gagcttgccg gggaaccggt aacgctgaac gacgtagagc
     2521 atcgcattct ccgccccatc tggcgcgacc accggatcca ctacgtcctg aactgcgcaa
     2581 gcctgggctg ccccaacctg agcggcgacg tctacacgcg ctcgagcctg gaggaccagc
     2641 tgcgcgaagc ggagacggct tacatcaacc acgagcgcgc ggtgaccttc aacgatggag
     2701 gaggactgcg tctttcgagc atctacaagt ggtaccggga cgacttcgcc cctgacagca
     2761 agggcctgct gagctacctc gcagaccacc atcagagtct ggccgatcgt cttcgcgccc
     2821 atgaccggga cgtggagttc gactacgact ggagtctgaa tcgggcggac tgaagtcggt
     2881 ggacgagcat tgcaccgcag cgatgcgaaa ggccggctct cctgcccgtt gcgcccctat
     2941 ctacctcggt cagcccctat ctacctcgga atgcgtgatg cagcgcggcg atgtggtcgg
     3001 gcgagaggcc gcaacaaccg cccagaatct gaaccccgtt gtcgacccaa ccccgtgcaa
     3061 accccacaag cgcctcaggc tcgatgatgt cgtcaaactg ccagttgggc atacgaaaat
     3121 agccagagtc cgggtacgcc atgagcattc ccggataccg ctccctcagg acagagatgg
     3181 cgtcggcgac gacatcggca gccgtatgca tcaacccggc ggcttcgact tcgccccact
     3241 cgagaactga cagtatgtcg gcgagcggaa cacaggcgtc gcgcccgaaa ccgacgacct
     3301 cgccccgctc gtcccggcgg gcggagaaac cggcccagac cggcaggccg gtcgcaagag
     3361 cggcctcgaa cgccagtgga acacgccgtg ggcaatacat catctccaga agtatgaggt
     3421 cgcagccggt gtcgcggaga agcatcgcga gttcgtcgaa cgcttcccgc atgacctcta
     3481 ccggtggctc gcgatcggcg tcggcccggt ccgtaccggc tgcaatcggg cacatgtgcg
     3541 acaacgaacc ggccacgagc acgtctgcgc ggccgctaag atcccgtgcc tcatgagcgg
     3601 cggtcaccgc ggcacgatta atctcctcga aacgatcgcc gcacccggcg ggctccagca
     3661 tcaatcgtga ggacgcgtac gtgttcgtgg tgatgatgtc ggcgccagac gagatgtaat
     3721 cgagatgaac atccttgagg acttctgcat tgtccaaagt tgccggtcca caccacgccg
     3781 cgggatccat gacggctcca cgcttctcca gctcggtccc ggtgccgcca tccaggagaa
     3841 cgatttctcc atcttgaagg cgactctcca tggccgagta gcgcccacct ggcgccatct
     3901 catgactgcg cttggcacgc acttccgacc cgggatcgag cttcgcaggc gtttggcggg
     3961 aggccgtcaa gcccacccca gttcttggag agttatgccg tcattccaga tcttcgtcat
     4021 gtgccgaata cgatccccat cgaaatccat cgcgtagacg taatccgcag ctgccttgcg
     4081 gcccgtcggt tcacccggcc cgccagggcc ggtgttggtt ccgtggaaca cggcgaaggc
     4141 cgcaacgcag gcccgttcct catcttccgc gaagaacttg agctcgtaac ggccgtcggg
     4201 aatcgggatc agcaggttct tcatccattc gcagtatcct tccagcgaat cgatgtcggc
     4261 caacgcgccg gactggcacg agaacgtggc tcgaggagtg cagtactctt tgcaaccctc
     4321 ccaacccttg ccggtttcgc aggcatcaaa gaacagtctt gcttggtcga gatttcgact
     4381 catgacaggc tccccttgca gtccgcccaa tcttgccacg aaaaccgacg gcgccctacc
     4441 tctaaagcac gggactccgg tcgccgccgc ccgagggggc ggcgatgggg tcacgccttc
     4501 cgggcaacct tatgagcacg ggagtcctgg aagtaagcat gctcgatctt cacttccata
     4561 ccggcacaca gctcagcaaa ggagatagcg cctgccgcct tggtcggagc gggcagctgc
     4621 agggccgaag cgatctcgat gatcggcttt tccgtgagcg ccgccatgac gtcgacgatc
     4681 tggggtaacg cctcgccctt cgtcgacttc tctcttcgag ccagagcttc tgccctctcc
     4741 tccggccagt ctccggtcca cacgtagcaa ttgggttcgg gcacggcggc aaagtcgcag
     4801 tacaccgaag gacgcagatt gttgaaggtc gccatcgagt gcggaacgtg aaatttctcg
     4861 ttctcggtgc acaggactga cccgcagcat tccgcgcgca agttgatgtt gagcgcatct
     4921 tccttgttca acctgaacac ggaaagcctg gcgagcgttt cccggcccgg ccgcatgatc
     4981 tgcgagtcga cgtacagcag atccacggcc tcttccaggt tcttgattgc agcggtcggt
     5041 tctccgttgg cgccgatgta ggcacgctga aggcagtccg tgcagcagca gccacaacgg
     5101 aatttgggaa cggggttggg caacgcgatc gtgacctcgc cgcagcgaca ggtgatcgtc
     5161 cccaccctgg tcgacgggct gcttgcgttc gcttccgaca tctctcagcg cctgtatgcg
     5221 gaaagcctgg acggaactat aaccgtctcg atcgtggacc tcacgaggac cggacgcgcc
     5281 agatcgaaga cgagcatccc tcaagccgga ggttgctcga actcactcga actgctgctc
     5341 gtaggattcc accgaggcaa acacttccac cgagccgtct ttgttgagca gcagcacctc
     5401 gtacggcatg aactgatcct tgtactccat gcccgggctg ccaaccggca tggccggcac
     5461 ggtgaggccc agagcgcccg caggcgggtc agccaggaat tgcgtgataa accgtgccgg
     5521 cacgtggcct tcgaagacgt agccatcctg gctcacgccc gtgtggcaag acccgtagcg
     5581 ggccggcacg ccgagctcgc gtttcagctc acctaagtct tgcgggtgat gaccggtgct
     5641 ggtgtaacca ctctcggcca cgtggtccat ccacttcacg caacagccgc aggtgggcga
     5701 cttgtagacg tccagatgca tggcggcgcc ctcttccgcc gccgtgaatc cggccgttgc
     5761 gaaccacagc cccaacccaa cggccatcgt agcgagcatc cgagccatgg cgttctccat
     5821 cggtgtctgc atgaggagcc gatcataagg ccggaagccc gtccaagccc aacaggaagc
     5881 atgcagatgg ggttcgagac tcgcctccgc aggatcccaa caggttctca gattgacgaa
     5941 cgccgcgcat cccggcctgc gcgatcccgc tagtatcagc tcttgcaaca cgagtgctca
     6001 gatgtcagat gtcgctagtc tttggctgct ctttggcctg cctcccgcag ctcccatcgt
     6061 cgctgctctg attgcctttg ctgctcgaga cacggggcgc tggcgttggc cgctggcgct
     6121 gagctatgtt gcattggtgt tctgtggcgc catcgcaaca acgaacatgg cgtatcacgt
     6181 cagagagggc tacaagaatc tgccggacgt gaactgggcc gtgtgggcag gtcttgtctg
     6241 ggcaggagca cttacgctgc cgagcgccat cgcgcttgcg ctacgctaca agtcgatggc
     6301 gacggccctg cttaccggtt tgggtgtagg cgttctgtcg gctatcactc tgatctcaac
     6361 cagtttcggt ctaatgatga tgctttcttt cgagtagcct tcgatcaccg ccccaacgcc
     6421 cagcgctgct ttcgtgcaag aggcggacgt caggatcggc atcagacgca cccgcacccg
     6481 ggcagccacc ggctaaattc acgtaacgat cactttcgcc tttttaagct agccagccaa
     6541 acttctgcca ggcccctacc agatgcgaac tacgcaaacc ctcttggcta cctgcatcct
     6601 gtttctcgcg gcagtgctgc tcatcggcgc tgcccccggc gtgcaggccg atcagagctt
     6661 ctacacctca aaaccagcca gccgcgacgg aatcggcaag ctctacatgg gccgcgagat
     6721 tgcccacgtg atggggcacc tgggcgcggg ttggctggaa cgacccgagc gtgagcggga
     6781 ggagcgaacg gatctgctgg tggcaagact cccggtggaa cccgatttcg tggtggccga
     6841 catcggcgcc ggcaccggct acttctcgtt ccccatcgcg gagcggttga cagacggtga
     6901 ggtgcttgct gtggacattc agcctcagat gctcgaaatg atcgagcggc agaaggcgaa
     6961 gcgcaacatc gacaacgtct caaccgtcct cggcaacgag atggacccgg ggctgcccga
     7021 atcgggcgtc gacctcatct tcatcgtcga cgcttatcac gaattttccc acccgcggga
     7081 gatgggcgag gccatgttcg ccgctctggt accgggtggg tatctggtgc tgatcgagta
     7141 ccgggccgaa gacgcctccg tcccgatcaa gcggctgcac aagatgtcgc aggcacagac
     7201 gaagaaagag atgcgggcaa tcggcctcga gtggttgcgc acggaggact acctgccaca
     7261 gcagcacgtg ctggtgtttc agaagcccgc agtcgggtcg ttggccgagc ctccggtcac
     7321 tgcgccttga gcgtgagctt catgcggacc cggtcgtcgt catcgaaatc gaccgccgcg
     7381 gcgctgcgat ccgcaatcac ctggcccatc ctgtcagcct tcaacaccgc cgcatcatcc
     7441 tcggacgact gaacaccacc aagttgggga ttcagcatgc agcgggctct gagagtggac
     7501 ggctcacgta tggggcgcag gcggctttaa cgtcaatctc cctcgaactc gcaaaggaag
     7561 tgcacctcca atcccatttc ctccagacgt ttctggccgc ccagatccgg aaggttcacc
     7621 acgaacgagg cggcgacgat atggccgccc agccgttcga tcagcctggc cccggcgacg
     7681 gcggtgccgc cagtggccag caggtcatcc agcaggagca cgcgcgcgcc ggcatgcagg
     7741 gcgtcgtcat gaatctccac cactgcgtca ccatattcca gggagtaggc ctcgacgatg
     7801 gtctttccgg gcagcttgcc tttcttgcga atgggaacga atggcaggcc gagcttgtaa
     7861 gccaccgcac cgccaagaat gaagccgcgt gcgtccattg ccaccactgc atcgatgcca
     7921 tcgccctcat atggttcggc aagctcgtcc accgcccgcc tgaacccttc ggcgtcgcca
     7981 aaaagcgtcg tgacgtcgcg gaactgaatt ccggggatgg ggaagtcggc gatcgttcgg
     8041 atatagtcgg taagcggctt ctggctca
//
LOCUS       DBNHKV010000342         7753 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_828748, whole genome shotgun sequence.
ACCESSION   DBNHKV010000342 DBNHKV010000000
VERSION     DBNHKV010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_828748"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="AC16PP_10920"
     CDS             <1..419
                     /locus_tag="AC16PP_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HIR6482183.1"
                     /translation="QVTAEEMVVFSPEGTTKASISVFTDVDCGYCQKLHLEVPKLNEM
                     GIEVRYLAYPRAGVGSQSYQKIVSAWCSADRNTAITRLKARQTIPTLTCSNPVASQLD
                     LGREVGVTGTPAIVLEDGRLLPGYMPAEQLAEALGI"
     gene            513..1829
                     /locus_tag="AC16PP_10925"
     CDS             513..1829
                     /locus_tag="AC16PP_10925"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015885695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR6482184.1"
                     /translation="MEPLKVGMCGLGTVAQGVLEVLATNRESITRRAGREIRVTRVAS
                     RRAKPDVDLGRAEFSTDVHALARDPDIDVLLELIGGEDTALELVTSALARGTPVVSGN
                     KAIVALHGNELLPLAAQHGAHLGFEAAVAGSIPIVSSLNRSLAANQHEWLAGIINGTS
                     NFILSAMTQSGRAFADALAEAQALGYAEADPTFDVEGIDAAHKLTILTALAFDVGFDF
                     HSVYTEGISKLTAEDIGYARQLGYRIKHLGIARRREHGIEARVHPVLVPEQHLLASIG
                     GAMNAVLVHSNAAGDTLYSGAGAGALPTASAVVADLIDLARGTARLPSPVGDVDLPIL
                     PIAETDSAYYLRIPSVDRPGAFARVATILSEREISIEAAIQREEARVRTRGAESGDGA
                     WVPIVILTHSVREAVMNEALEAVQALPDVVGEIMRIRVEHFDEDGE"
     gene            1826..3550
                     /gene="recJ"
                     /locus_tag="AC16PP_10930"
     CDS             1826..3550
                     /gene="recJ"
                     /locus_tag="AC16PP_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007250101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR6482185.1"
                     /translation="MTQTGPGPQIVTRPGADADRLPHLPPLLARIYAARGIVEAADVD
                     HALAGLHAPDTLPDIQRAGQRLARAVMEDERILVVGDFDADGATSVALALTLLRALGA
                     AQVEFMVPNRFDFGYGLSPEIVDLAARRQPAVLVTVDNGVSSVAGVARANEHGIDVIV
                     TDHHLPGDVRPDAYAIVNPNLPESAFPSRSVAGVGVIFYVLSVVRSELRERGWFAGRE
                     EPKMAEWLDLVALGTVADVVPLDRNNRIFIEQGLKRIRGGRARPGIRALCEVARRDAG
                     KLTAADLGFALGPRLNAAGRLDDMSLGIRCLTAADPGSARELTTALDELNRARRQLQE
                     EMVSDAELLVAGHDVAVEDRYGVCVYDANWHQGVVGIVAGRLREKLHRPVIAFAEAGD
                     LAPDELKGSARSLPDLHVRDALDAVATRYPGMLVNFGGHAMAAGLTLKRVHYEAFARA
                     FDAEVRRVLPAESLQAVIVTDGRLEDEELSLDVARLLAAAGPWGQGFPEPCFHDEFTV
                     VSQRVVGESHLKLVLKRGERLFDAIAFNQPPLPDVQRVAAVFRLSENDYGALPTLQLM
                     VEHLVGLA"
     gene            3603..4701
                     /gene="prfB"
                     /locus_tag="AC16PP_10935"
     CDS             join(3603..3677,3679..4701)
                     /gene="prfB"
                     /locus_tag="AC16PP_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153889742.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR6482186.1"
                     /translation="MAEITSVRERIKDLSERDVALRGYLDFAEKEHRLAEVELTLAEP
                     DVWNDPEEAQRLNKERSALEQVVKGLRDLHETLNDLRDLAELASTEEDHDALTEVDEE
                     LDDLERQLESLEFRRMFQGEMDEANAYVEIQSGSGGTEAQDWAEMLLRMYLRWGEKHG
                     FDTEITELSPGEVAGIKGATVQFQGDHAFGWLRTETGVHRLVRKSPFDSGNRRHTSFA
                     SVFVAPEIDDDIEIDINPADVRVDTYRASGAGGQHVNRTDSAVRLTHLPTNTVVQCQS
                     ERSQHQNKDKAFKQLRAKLYELAELERNAERQAVEDSKADIGWGSQIRSYVLDQSRVK
                     DLRTHVERSDPGNVLDGDLDEFIEASLKAGL"
     gene            4714..6201
                     /gene="lysS"
                     /locus_tag="AC16PP_10940"
     CDS             4714..6201
                     /gene="lysS"
                     /locus_tag="AC16PP_10940"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR6482187.1"
                     /translation="MTDESDIIRHRREKLARWRENGRAYDNQFRRDALAQELHARCVD
                     LDKDALADAGIQVAVAGRVMLRRVMGKASFITLKDTSGTIQCYLRTEDVGPEAYEEFD
                     DLWDIGDIVGVRGTMMRTNKGELTVAAASIELLNKTLRPLPEKYHGLTDHEIRYRQRY
                     LDLIMNDDTRRVFEIRSRVIGKIREFMQERGFLEVETPMMQVIPGGATARPFVTHHNA
                     LDLELYLRVAPELFLKRLTVGGMERVFEINRNFRNEGLSTRHNPEFTMLEFYWAYADF
                     EDLIALTEELLQLLARDVLGTGTLTYQGTEIDLARPAERYTMVEAIERHTGIASGQLK
                     DPDALAAILKGMNVQPEADWGIGKMQLELFEARVEDRLVQPTFITHYPTEVSPLSRRN
                     DEDPEVADRFELFIAGREIANGFSELNDPEDQAERFRAQALLRSGGDVEAMHFDDDYI
                     TALEYGLPPTAGEGLGIDRLVMLLTDSPTIRDVLLFPLMRPVQDG"
     gene            6194..6910
                     /gene="ung"
                     /locus_tag="AC16PP_10945"
     CDS             6194..6910
                     /gene="ung"
                     /locus_tag="AC16PP_10945"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR6482188.1"
                     /translation="MVEAARRSEERVRLEPSWKAVLGGEFDKPYMNKLREFLLREKRA
                     GKVIYPRGDNIFAALDLTPLEDVKVVIIGQDPYHGPDQAHGLCFSVQPGIPQPPSLVN
                     IFQEINEELGGPAKRLDDTNGCLEPWARQGVLLLNAVLTVERGRAGSHQGMGWEIFTD
                     KVVDVLNRERDGLVFLLWGSYAQKKGAILDVQRHCVLKAPHPSPLSAHRGFFGCGHFA
                     RANEYLQQRGVAGIDWFDVA"
     gene            6966..>7753
                     /locus_tag="AC16PP_10950"
     CDS             6966..>7753
                     /locus_tag="AC16PP_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR6482189.1"
                     /translation="MIESPTEKLLVEQRGHTLLVTINTPPANTWDAESLQGLKNVVEQ
                     ANAERSVYAIVITGQGEKFFSAGADLKLFADGDPAVASEMAGQFGSAFETLSAFRGVS
                     IAAVNGFAMGGGLECALACDIRVAEAQAKLALPEATVGLLPCGGGTQNLAWMVGEGWA
                     KRMILCGERVDAATALKIGLVEEVVDAGQSLGRALELAEMVGRQSPPAVAACKDLIQQ
                     ARSGNISKAYSTERAAFVGLFDTQDQKEGVNAFLEKRSPEWVNG"
BASE COUNT         1392 a         2401 c         2620 g         1340 t
ORIGIN      
        1 cccaggtgac ggcggaggag atggtggttt tctccccgga agggacaacc aaggcgtcca
       61 tcagcgtgtt cacggacgtg gactgtggtt actgtcagaa gctgcacctc gaggtgccca
      121 agctcaacga gatgggcatc gaggtccggt acctggccta tccgcgggcc ggggtgggtt
      181 ctcagtcgta tcagaaaatc gtgtcggcct ggtgctcggc cgatcgcaac acggccatta
      241 ccaggctgaa ggcgcgacag accattccca cgctgacctg ctcaaacccg gtggcttccc
      301 agctcgacct cgggcgcgaa gtgggcgtga cgggcacacc ggccatcgtt ctcgaggacg
      361 ggcggctgct gcccggctac atgccggccg agcagctggc ggaagcgctg ggcatctgat
      421 cccctcgggc gaagtttccc gtcgatacct ttgagcacag gacggcgcgc cagtagactt
      481 ggcgcctcgt cgcagcatct tggtcattgg cgttggaacc cctcaaggta ggaatgtgtg
      541 gtctaggcac cgtggcacag ggcgtgctgg aggtgctggc caccaatagg gaaagcatta
      601 cccggcgggc gggccgggaa atacgggtga cccgcgtggc cagccggcgg gccaagccgg
      661 acgtggacct tggccgggcc gagttttcca ccgacgtgca tgccctcgcc agggatcccg
      721 acatcgatgt tctcctagag ctcattgggg gtgaggatac cgccctggag ctcgtcacca
      781 gtgccctcgc caggggaacg ccggtggttt cgggtaacaa agccatcgtg gcgctgcacg
      841 gaaacgagct cctgccgttg gcagcccagc acggcgcgca cctcgggttc gaagccgcgg
      901 tagcgggcag cataccgatc gtttcaagcc tcaaccggtc tctggccgcc aaccagcatg
      961 aatggctggc cggcatcatc aacggaacct ccaatttcat tctcagcgcc atgacccaga
     1021 gtgggcgggc cttcgccgat gcgctggccg aggcgcaggc gctcggctac gccgaagccg
     1081 accccacctt cgacgtggag ggcatcgacg ctgcccacaa gctgacgatc ctcacggcgc
     1141 tcgccttcga tgtcggcttc gactttcact cggtctatac ggagggcatc tcgaagctga
     1201 cagccgagga tatcggctat gcccggcaac tcggctatcg catcaagcac ctcggcatag
     1261 cgcggcgccg cgagcacggc atcgaagctc gcgtgcaccc cgtgctggtg ccggagcagc
     1321 atctgctcgc cagtatcggc ggtgcgatga acgccgtcct ggtgcacagc aatgccgcgg
     1381 gcgatacgct ctacagcggt gcgggagccg gcgcgctgcc gacggcctca gccgtggtcg
     1441 ctgacctcat tgatctcgct cgtggcactg ccagattgcc cagcccggtc ggagacgtcg
     1501 accttcccat tctgccgatc gccgagaccg acagcgccta ttacctgcgg attccctccg
     1561 tcgaccgtcc gggcgcgttc gcccgggttg cgaccattct cagcgaacgg gagatcagca
     1621 tcgaagcggc cattcagcgg gaagaagcca gagttcggac ccgcggagcg gaatcgggcg
     1681 atggagcctg ggtgcctatc gtgattctga cccacagcgt gcgcgaggcc gtcatgaatg
     1741 aagcgctcga ggccgtgcag gctctgccgg acgtcgtcgg cgagatcatg cgcatccggg
     1801 tagagcactt cgacgaagac ggcgaatgac gcagacgggg cctggcccgc agatcgttac
     1861 ccgtccggga gccgacgcgg atcggctccc gcacctgccg ccgctgctcg cgaggatcta
     1921 cgccgcgcgg ggtatcgtcg aggcagcaga cgtcgatcat gcgctcgccg ggctccacgc
     1981 gcccgacacg ctgcccgaca ttcagcgggc cggtcagcgt cttgcccgtg cggtcatgga
     2041 agatgagcgg attctcgtgg tgggcgactt cgacgccgac ggcgctacgt ccgtggcgct
     2101 cgcccttact ctccttcgcg ccctcggggc ggcgcaggtg gagttcatgg tgccgaaccg
     2161 gttcgacttc gggtacgggc tttctccgga gatcgtcgat ctcgccgcgc gaaggcagcc
     2221 ggccgttctg gttaccgtcg acaatggcgt gtccagcgtc gcgggggtgg cacgcgccaa
     2281 cgagcatggc atcgacgtga tcgtcaccga tcatcatctg cccggcgacg tgcggccgga
     2341 tgcgtacgcc atcgtcaatc ctaatcttcc cgagtcggcc tttccgagcc ggtcggtcgc
     2401 cggggtcgga gtaatcttct acgtgctgag cgtggtgcgc tccgagttgc gggagcgagg
     2461 ttggttcgcc ggccgcgaag agcccaagat ggcagagtgg ctcgacctgg tggcgctcgg
     2521 cacggtggcc gacgtggtgc ccctggatcg caacaaccgc atcttcatcg agcaggggct
     2581 gaagcggatc cgtggaggtc gcgctcggcc gggcatcaga gcgctctgcg aggtggcgcg
     2641 ccgcgacgcg ggaaagctga cggcggccga tctcggcttt gcactgggcc ctcggctgaa
     2701 tgcggccggc cggctggacg acatgagcct gggcatacgc tgcctgacag ctgctgatcc
     2761 cggttcggcg cgtgagctca ccacggcgtt ggatgagctc aatcgcgccc ggcggcagct
     2821 gcaggaagag atggtcagcg acgccgaatt gctggtggcc ggccacgacg tcgccgttga
     2881 ggatcgttac ggcgtctgtg tttacgacgc gaactggcat cagggcgttg tcggcatcgt
     2941 ggccggccgg ctgagagaga agctgcatcg cccggtcatc gccttcgcag aggccggcga
     3001 tctcgcgccg gacgagctca agggttccgc ccgctcgctg cccgatctgc acgtaaggga
     3061 cgctctggac gccgttgcca ctcgttaccc cgggatgctg gtcaatttcg gtggtcacgc
     3121 catggcggcc gggctgactc tgaaacgcgt gcactacgag gcttttgccc gggcgttcga
     3181 cgccgaagtc cggcgggtgc ttccggcgga gtccctgcag gctgtgatcg tgacggacgg
     3241 acggctggag gacgaggagc tgagcctgga cgtcgcccgc ctgctcgccg ctgccgggcc
     3301 ctggggccag gggtttccgg agccctgctt tcacgacgag ttcaccgtgg tcagccagcg
     3361 ggtggtcggg gagtcccatc tcaagctggt gctcaaacgc ggggaacggc tcttcgacgc
     3421 catcgccttc aaccagccgc cgctgcccga cgttcagcgc gtggccgcgg tgttccgtct
     3481 cagcgagaac gactacggcg cgctgcccac gcttcagctc atggtggagc atctggtggg
     3541 gttggcataa acttgcgcgt cttttgcggc cgggcgccgc aggtctgtcg agagcgggga
     3601 taatggccga gatcacgtcg gtgcgagagc gtatcaagga cctcagcgag cgcgacgtcg
     3661 cgcttagggg gtacctttga ctttgccgag aaggaacacc gtctcgccga ggtcgagctg
     3721 accctcgccg agcccgacgt ctggaacgat cctgaagaag cgcagcgcct caacaaggag
     3781 cgctcggcgc tggagcaggt ggtcaaaggc ctgcgcgatc tgcacgagac cctcaatgac
     3841 ctgcgggatc tggccgagct tgcgtctacc gaagaagacc acgatgccct gacggaggtc
     3901 gacgaggagc tcgacgatct cgagcggcag ctcgaatccc tggagttccg acgcatgttc
     3961 cagggcgaga tggacgaggc gaacgcgtac gtcgagattc agtccggatc cggcggcact
     4021 gaggcccagg actgggcgga aatgctcctt cgcatgtatc tgcgctgggg agaaaagcac
     4081 ggcttcgaca ccgagatcac cgagctatcg cccggcgagg ttgccggcat caagggggcc
     4141 acggtgcagt tccagggtga tcacgcgttc ggctggttgc gaacggaaac cggggtgcac
     4201 cggctggttc gaaagtcgcc tttcgactcc ggtaatcggc gtcacacctc gtttgcttcg
     4261 gttttcgtcg cgcccgagat cgacgacgac atcgagatcg atatcaatcc tgccgatgtg
     4321 cgggtggaca cgtatcgggc cagcggggcc ggcggccagc acgtcaaccg gacggactcg
     4381 gccgttcgcc tgacccacct gccaacgaac acggtcgtgc agtgccagag cgagcgttcg
     4441 cagcaccaga acaaggacaa ggcattcaag cagctgcgcg caaaactcta cgagctggcc
     4501 gagctcgaac gcaacgcgga gcggcaggcc gtcgaggact cgaaggccga catcggctgg
     4561 ggcagccaga tccgctccta cgtgctcgac cagtcccggg taaaggacct gcgcacccac
     4621 gtagagcgat cggatccggg taacgtgcta gacggcgacc tggatgaatt catcgaagcg
     4681 agcctcaaag caggcttgta gggcttaatt ctcatgaccg acgaatcgga catcatcaga
     4741 catcggcgcg agaagctcgc gcgctggcgc gaaaacggac gggcctacga caatcagttc
     4801 cgccgcgacg cccttgcgca ggagctgcat gctcgctgcg tcgacctgga caaggacgcg
     4861 ctggccgatg ccggcatcca ggtggccgtc gccgggcgtg tgatgctgcg acgggtgatg
     4921 ggaaaggcga gcttcatcac tctcaaggat acgagcggca cgatccagtg ctacctgaga
     4981 acggaagacg tcggcccgga agcctacgag gaattcgacg acctctggga catcggcgac
     5041 atcgtgggcg tgcggggcac catgatgcgc acgaacaaag gcgagctgac cgtcgctgcc
     5101 gcgagcatcg agctgctcaa caaaactctg cggccccttc cggagaagta ccacgggctc
     5161 accgatcacg agatccgtta tcgccagcgt tatctggatc tcatcatgaa cgacgatacc
     5221 cggcgggtct tcgagatccg ctccagggtg atcggcaaga tccgcgaatt tatgcaggag
     5281 cggggttttc tggaagtcga aacgccgatg atgcaggtga tacccggcgg cgcgacagcg
     5341 cggcctttcg tcacgcatca caatgcgctc gatctcgagc tgtatctgcg tgtcgccccc
     5401 gagctcttct tgaagcgcct gaccgtggga ggcatggagc gcgtcttcga gatcaaccgc
     5461 aacttccgca acgaagggtt gtcgacgcgg cacaacccgg agttcaccat gctggagttc
     5521 tactgggcct acgccgattt cgaagacctc atcgctctga ccgaggagtt gctgcagctt
     5581 ctcgcccggg acgttctcgg gacggggacg ctcacctatc aggggacgga aatcgatctg
     5641 gcgcggccgg cggaacgcta caccatggtc gaagccatcg aacggcacac gggcattgcc
     5701 tcggggcagc ttaaggaccc cgatgccctg gccgccattc tcaagggcat gaacgttcag
     5761 cccgaggcgg actggggtat cggcaagatg cagctcgaac tcttcgaggc cagggtggaa
     5821 gaccgcctgg tccagcccac gttcatcacc cactacccca ccgaggtatc gccgctgtct
     5881 cgccgtaacg acgaggatcc tgaggtggcc gaccgtttcg agctcttcat cgccgggcgc
     5941 gagatcgcca acggattttc cgagctcaat gacccggagg atcaggccga gcgcttccgc
     6001 gctcaggcgc tgcttcgaag cggcggcgac gtggaggcca tgcatttcga cgacgattac
     6061 atcaccgcgc tcgaatacgg tctgccgccc acggccggcg aggggctggg catcgatcgg
     6121 ctggtgatgc tgctcacgga ctccccgacc atccgggacg tgctgctgtt tccgctgatg
     6181 cggccggtgc aagatggttg aggccgcccg ccggagcgag gagcgcgtcc gcctggagcc
     6241 ttcctggaaa gcggtgcttg gcggtgagtt cgacaagccc tacatgaaca agctgcggga
     6301 gttcctgctc cgggagaagc gggcgggcaa ggtgatctat ccccggggcg ataacatctt
     6361 cgccgccctg gacctgacgc ccttggagga cgtcaaagtc gtgatcatcg gtcaggatcc
     6421 ctatcacggc cccgaccagg cacacggcct gtgcttctcg gtgcagcccg gcatcccgca
     6481 accgccatcc ctcgtcaaca tctttcagga gatcaacgag gagctcggcg gtcccgcaaa
     6541 gcgtctggat gacaccaacg gctgtctgga gccgtgggcc aggcagggcg tgctgctgct
     6601 caacgcggtg ctgacggtgg agcgtggtcg cgccggctcg catcagggca tgggctggga
     6661 gatctttacc gacaaggtgg tcgacgttct caaccgggag cgggacgggc tggtctttct
     6721 gctgtgggga agctacgcgc agaagaaagg agccatcctg gacgtgcaac ggcattgcgt
     6781 cctgaaggcg ccccaccctt cgccgctgtc tgcccaccgt ggttttttcg gctgcgggca
     6841 ttttgcccgg gccaacgagt accttcagca acggggcgtg gccgggatag actggttcga
     6901 cgttgcgtga tgcgactatc tggaagaaaa caccgaactg atgatcgact caagagagga
     6961 agctgatgat tgaatcgccg actgaaaagc tgctggtcga gcagcgaggg cacacgctgc
     7021 tcgtcaccat caacacgccg cccgcaaata cctgggatgc ggaaagtctg caggggctca
     7081 agaacgtcgt cgagcaggcc aacgcagaac gcagcgttta cgccatcgtc atcaccggtc
     7141 agggtgagaa gttcttctcg gcgggggccg atctgaagct gtttgcagac ggggatccgg
     7201 cagtggcctc ggagatggcc ggccagttcg gctcggcttt cgagacgttg tccgcgtttc
     7261 gcggggtcag catcgccgcg gtgaacggct tcgccatggg cggcgggctg gagtgcgcgc
     7321 tggcctgcga catccgtgtt gcggaggcgc aggcgaagct ggcgttgccc gaggccacgg
     7381 tgggtctgtt gccgtgcggg ggcggaaccc agaacctggc ctggatggtc ggcgaaggat
     7441 gggcgaaacg catgattctc tgcggcgagc gggtggatgc ggcaaccgcg ttgaagatcg
     7501 gcctcgtcga ggaggtggtg gatgccggcc agtcgcttgg tcgcgcgctc gagctggcgg
     7561 agatggtggg ccggcagagc ccgcccgcgg tggccgcctg caaggatctc attcagcagg
     7621 cgcgcagcgg caacatcagc aaagcctact cgacggagcg ggccgccttc gtgggtctgt
     7681 tcgatacgca ggaccagaaa gaaggggtca acgctttcct cgagaagcgt tcgcctgaat
     7741 gggtcaacgg gta
//
LOCUS       DBNHKV010000343         6564 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_189809, whole genome shotgun sequence.
ACCESSION   DBNHKV010000343 DBNHKV010000000
VERSION     DBNHKV010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_189809"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="AC16PP_10955"
     CDS             complement(<1..512)
                     /locus_tag="AC16PP_10955"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6482190.1"
                     /translation="MLDDLFSKKPLSLAVASAIAVSQPMAVAAATGQIEELVVTATKR
                     AESTQDIPVSVQAITGEDMKEAGVATFEEYIVYLPNVVRSGRGPGKNDLYIRGAATEQ
                     AGVTVSSANGTTPAVALYLDEQPVSFGGRNLDIYAADLQRVEVLPGPQGTLFGVSSQA
                     GTVRLITNKP"
     gene            899..1879
                     /locus_tag="AC16PP_10960"
     CDS             899..1879
                     /locus_tag="AC16PP_10960"
                     /inference="COORDINATES: protein motif:HMM:NF016974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HIR6482191.1"
                     /translation="MELSHPFIRLPFRFDADRLASELADIDDAAWLPHPSGMPGNSAV
                     ALVSRDGGDNDEFQGTMQPTGHLRRSPYMAQVMGSFGEILARSRLMRLSSKCEVQMHV
                     DFNYHWYTRVRIHIPIVTDPRVTFCCADERVHMAPGECWIFDSWRRHRVINASDSDRV
                     HLVVDMSGSSRFWRMVRHVQGSPDHAEEEFVAFDPGMQVSLSTERYNTAPVMAPGEVD
                     ALVKELVVDFSSHPDNDPGLVRRYALLLGDFCMDWRQIWLQHGYEREGIASYAALIDG
                     VQRQLHPNPRALVTSSNQVGVNPIIVQRILRPALAPEHLDDYLHDVRANG"
     gene            1949..2428
                     /locus_tag="AC16PP_10965"
     CDS             1949..2428
                     /locus_tag="AC16PP_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6482192.1"
                     /translation="MSEEEQVLITIRKIIRATDIYSRQLSKTVGLTVPQLLVLQAIRD
                     LGAVAISKISSEISLSQATVTSIIDRLEVRGLVSRRRSEADKRVVHAFLTEPGAELLD
                     SAPTPLQEVFRSRFDALEAWEKSMIIASLQRVAAMMDAEQLDASPMLAVGSPDPAAS"
     gene            complement(2425..3036)
                     /locus_tag="AC16PP_10970"
     CDS             complement(2425..3036)
                     /locus_tag="AC16PP_10970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6482193.1"
                     /translation="MGLYAKHVLPRMIDVLCGQPPMTELRRRYVPQASGDVLEIGIGS
                     GHNLAHYSSRVSSVTGLDPAAELTVMAQERANGLHTDVKLLQVSGEEIPADDARYDSL
                     VCTWTLCSIPNVYAAVREMRRVLKPGGRLYFVEHGASPDLPVARWQRRIEPVWKKIGG
                     GCHLTRRADELLQDAGFKLAELDTGYLPGPRFAAFMIHGIAER"
     gene            complement(3046..4611)
                     /locus_tag="AC16PP_10975"
     CDS             complement(3046..4611)
                     /locus_tag="AC16PP_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6482194.1"
                     /translation="MTHFPQRIAADRGDAPALTDERGSTSWAELNARSNQLVHAFRAM
                     GLVPGDAIAIYAGNCREYYEVMMAANHAGLIYVPVNWHFSPEELAYVIDNAGARLLVT
                     EGQFLANAEAAVARGETPGLEQCVVIRPEADTTAFTDYEAFIGDQSTAEPDDQGAGGP
                     MFYTSGTTGRPKGVKSSTFAPGGPLAVLEMIGSGLSQMLQIPTDGTTYICGPVYHSAQ
                     WAFSFLPLISGSHVVMRHRFDPAESLQVMDAHEVTNVHLVPTQFHRLLRLSDDERAGF
                     SGSSLQVVWHGAAPCPPDVKRRMIQWWGPVVAEYYGSTEGSIVTTASASQWLERPGTV
                     GKPSPLVEVTIRDDDGQPLPANESGQIYVKSLMGSDFEYHNEPAKTAEVHLEPGTYTF
                     GDIGYLDDDGYLYLSDRKIDMIISGGVNIYPAEIEAVLVNHPAVEDAAVFGIPNDEFG
                     EEVKAAIKLRPGQSGNEELSADIIGFCRDHLAHYKAPKSIDYEDEFPRHETGKLYKRL
                     LRDRYWQDADRKI"
     gene            complement(4628..5512)
                     /locus_tag="AC16PP_10980"
     CDS             complement(4628..5512)
                     /locus_tag="AC16PP_10980"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015649522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6482195.1"
                     /translation="MTITVRPLTPVIGAEVSGADLTDLGDQTFADIDRALTEHLVLFF
                     RDQPRLEPDVQIAFARRFGDLHIHPAAPHLEGYPEIFVIHTDRNSKVANGNGWHTDVS
                     CDEQPPMATMLQLHVLPATGGDTLFASMYGAYETLSAAMRQFLAGLTATHASEHVYRG
                     RYEDRGVEDAGRTYPSAVHPVVRTHPRSGRQALYVNPSFTTRINELSGQESRTLLKML
                     YRHQQRAEFQVRFQWTPNAIALWDNRCTQHFALWDYWPAERKGHRVTIGGERPFYEPG
                     AEKPQAAGLRLASELSSQ"
     gene            complement(5522..>6564)
                     /locus_tag="AC16PP_10985"
     CDS             complement(5522..>6564)
                     /locus_tag="AC16PP_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIR6482196.1"
                     /translation="AAAAPAAITRGDGERILSSPAARRLAGELGVDISTLAGTGPGGR
                     IVEADVQAAAAGAGEERGSRSSPMARKVARELGVDIATVTGTGPGGRITKEDVELAAK
                     APPAAAPAAREAVASRGDQTVPVRGMRKTIAARMHESLQSMAQLTMDMDVIMDDAVKL
                     RGQLIEEWADEGVRPTYTDLVIRAAAKALEKHPLMNSVFGETEMTLKGEVNVGMAVAL
                     EEGLIVPVVRHANVLNMKELARESSRLAAACRDGNLTPDDLQGGTFTVSALGMFGVDS
                     FTPIINSPQVGILGVNRLRDDVAWDGAVPVKRQAMRLSLTWDHRALDGAPAAQFLATV
                     RDLLEAPYRLLV"
BASE COUNT         1061 a         2078 c         2199 g         1226 t
ORIGIN      
        1 ttgggcttgt tggtgatcag gcgcacggtg cctgcctggg agctgacccc gaacagcgtc
       61 ccctggggcc ctggcagaac ctccacccgc tgcaggtcgg cggcgtagat gtccagattt
      121 ctgccgccga aggacaccgg ctgctcgtcc agatacaggg ccaccgcggg cgtcgttccg
      181 ttggcggagg acacggtaac cccggcctgc tccgtggcgg cgccgcggat gtacaggtcg
      241 ttcttgccgg gcccgcggcc gctgcgcacc acgttgggca ggtagacgat gtactcctcg
      301 aacgtcgcaa caccggcctc cttcatgtcc tcgccggtga tggcctggac ggataccggg
      361 atgtcctgag tggactcggc gcgtttggtg gcggtgacca cgagctcttc gatctggccg
      421 gtcgccgcgg caaccgccat gggctgagat accgcgattg cggaggctac ggccagtgaa
      481 aggggtttct tcgaaaatag atcatcgagc atgtacatgt ctctccagta cgctgagtgg
      541 gcctgcgtcg cgtcgtcaat acggcttgct atggtggtcg cgctcgttaa aggaacgcgg
      601 accgggcgcc tatacggctg gtatcgacgc caaatcgatg tgagttaaat agattagggc
      661 cctaagtgtc tatgttactc aaatccgacg agaataccca ttatttctca attattgggg
      721 tttaaggccg cgtgactgca gatctatata caaatgaatc gaaataatta tcagagtttg
      781 tcgtctgttt tgtcggaaag ttggtaggct gcgaagcaaa tttcaggtac ggcctcagac
      841 aaggaaggct cgcttgccgg actcggcgcg gttcgacccg gacgggattc gttgatccat
      901 ggaactctca cacccattca tccggctgcc attcagattc gacgcggaca ggctcgcctc
      961 cgagttggcc gacatcgacg atgccgcatg gttgccccac ccttctggaa tgcccggcaa
     1021 ttcagccgtc gcgctcgttt cccgcgacgg gggtgacaac gacgagttcc agggcacgat
     1081 gcagccgacg gggcatctgc gtcggtctcc ctacatggcg caggtcatgg gatctttcgg
     1141 cgagatcctc gcccgatcac gcctgatgcg tctgtcctcg aaatgcgaag tgcagatgca
     1201 cgtcgatttc aactatcact ggtacacgcg ggtccggatt cacatcccca tcgttaccga
     1261 tccgcgggtg accttctgtt gtgctgatga acgcgttcac atggcgcccg gcgagtgctg
     1321 gatattcgat tcgtggcgcc gacatcgggt gatcaacgcc agcgactccg accgcgttca
     1381 cctcgtggtg gatatgtctg gttcctcgcg gttctggcgg atggtccgcc atgtccaggg
     1441 ctcgccggat cacgccgagg aagagttcgt tgctttcgat cctggcatgc aggtctcatt
     1501 gtccacggag cgttacaaca ccgcgccggt catggcccca ggcgaggtcg acgccctggt
     1561 caaggaactc gtggtcgatt tttcgtcgca tcccgacaac gatccggggc tggttcgacg
     1621 ctacgcgctg ctcctcggcg atttctgcat ggactggcgg cagatctggc ttcagcacgg
     1681 ctacgagcgg gaaggaattg ccagctacgc ggcgctgatc gacggcgtgc agcgccagtt
     1741 gcacccgaac ccccgggcgc tggtcacgag cagcaatcag gtgggggtca atcccatcat
     1801 cgttcagcgg attctgcgtc cggcgctggc accggagcat ctcgacgatt acctccacga
     1861 tgtgcgggcg aacggataac caaagctcgc cgtggcaggc gggcgttggt aaggtttcgc
     1921 tgtccatcag tcgtggcccg ggttggagat gagcgaagaa gaacaggttc tgataacgat
     1981 ccggaagatc atccgggcaa ccgacatcta ctcccgacag ctgagcaaga ctgttgggct
     2041 caccgtcccg cagttgctgg tcctgcaggc gataagggac ctcggtgccg tggcgatttc
     2101 gaagatatcc agtgagatca gcctgagtca ggccaccgtc acctctatca tcgaccggct
     2161 tgaagtccga ggtctggtct cgcggcggcg cagcgaggcg gacaagcggg tcgtgcacgc
     2221 tttcctgacg gagcccgggg ccgagctgct cgattccgcg ccgacgccgt tgcaggaagt
     2281 ctttcgaagc cgattcgatg ctctcgaagc ctgggagaaa tccatgatca tcgcgtcgct
     2341 gcagcgggtg gcggccatga tggacgccga gcagctcgac gcatcaccca tgctggccgt
     2401 tggcagccct gaccccgcgg cgagctagcg ctcggcgatc ccgtggatca tgaacgccgc
     2461 gaagcgcggg cccggaaggt agccggtgtc gagctcggcc agcttgaagc cggcgtcctg
     2521 aagcagctca tcggcgcgtc gcgtcaggtg gcagccaccg ccgatcttct tccagaccgg
     2581 ctcgatgcgt cgctgccagc gggcaaccgg caggtcgggc gaggcgccgt gctcgacgaa
     2641 gtacaggcgc ccgcctggct tcagcacgcg acgcatctcg cgcacggccg cgtagacgtt
     2701 ggggatcgag cacagggtcc aggtgcacac cagggagtcg tagcgcgcgt cgtcggccgg
     2761 tatctcctcg cctgagacct gcagaagctt gacgtccgtg tgcagcccgt tggctctctc
     2821 ctgagccatg accgtcagct cggcggccgg atccaggccc gttaccgagg acaccctgga
     2881 cgagtagtgc gccagattgt ggcccgatcc gatgccgatc tcgagcacgt cgccgctggc
     2941 ctggggcacg tagcggcgcc tgagctccgt catgggcggc tggccgcaga gaacgtcgat
     3001 catccgcggg agaacgtgct tggcgtagag tcccatggtc gttcctcaga tcttccggtc
     3061 cgcgtcctgc cagtagcggt cgcgcaggag ccgcttgtag agcttacccg tctcgtggcg
     3121 cggaaactcg tcctcgtagt cgatggattt cggcgctttg tagtgggcga ggtggtcccg
     3181 gcagaagccg atgatgtcgg cgctgagctc ctcgttgccg ctctgtcccg ggcgcagctt
     3241 gatggcggcc ttgacctcct cgccgaactc atcgttgggg atgccgaaca cggccgcgtc
     3301 ttccaccgcg ggatgattga ccagcacggc ctcgatttcc gcggggtaga tgttgacccc
     3361 gcccgagatg atcatgtcga tcttgcggtc gctcaagtag aggtagccgt cgtcgtccag
     3421 gtagccgatg tcaccgaagg tgtaggtgcc tggctccaga tgcacctcgg cggtcttggc
     3481 cggctcgttg tggtactcga agtcggagcc catcaggctt ttgacgtaga tctgaccgga
     3541 ctcgttggcc ggcaggggct ggccgtcgtc gtcgcggatg gtcacctcca ccagtgggga
     3601 cggcttgccc acggtgcccg gccgctccag ccactgcgaa gcgctcgccg tggtcacgat
     3661 ggagccctcg gtggagccgt aatactcggc gaccacgggc ccccaccact gaatcatgcg
     3721 tcgcttgacg tcgggcgggc agggcgccgc gccgtgccag actacctgca ggctcgatcc
     3781 cgagaagccg gcgcgctcgt cgtcgctcag gcgcagcagc cggtggaact gggtcggaac
     3841 gagatgtacg ttggtcacct cgtgggcgtc catcacctgc aggctctcgg ccgggtcgaa
     3901 gcggtggcgc atgaccacgt gactgccgct gatcagcggc agaaaggaaa acgcccactg
     3961 cgcggagtgg tagacgggcc cgcagatgta ggtggtcccg tccgtgggga tctgcagcat
     4021 ctgcgacagg cccgagccga tcatctccag gacggccagt ggcccgccgg gggcgaaggt
     4081 gctcgacttc acgcccttgg gtcgcccggt ggtccccgag gtgtagaaca tgggtccgcc
     4141 ggcgccctgg tcgtcgggct ccgccgtgct ctggtcgccg atgaacgcct cgtagtcggt
     4201 aaacgccgtg gtgtctgcct cgggccggat gaccacgcac tgctcgagcc cgggcgtttc
     4261 gcccctggcc accgcggctt cggcgttggc gagaaactgc ccctcggtca cgagcaggcg
     4321 cgcgccggcg ttgtcgatca cgtaggcaag ctcctccgga gaaaagtgcc agttcacggg
     4381 cacgtagatc aatccggcat ggttggcggc catcatcacc tcgtagtact cgcggcagtt
     4441 gccggcgtag atggcgatgg cgtcgcccgg gacgagcccc atggcgcgga aggcatgaac
     4501 gagctggttg ctccgggcgt tcagctcggc ccaggacgtg ctgccccgtt cgtcggtgag
     4561 cgccggggcg tctccccggt cggcggcgat tcgttgcgga aagtgcgtca tcggtcggtt
     4621 ccgtggttca ttgggaggaa agttcgctgg cgagccggag cccggccgcc tggggctttt
     4681 cagccccggg ttcgtagaag gggcgctcgc cgcctatggt gacccggtgt cccttgcgct
     4741 cggcgggcca gtagtcccag agcgcgaaat gctgcgtgca gcgattgtcc cacagggcga
     4801 tggcgttcgg cgtccactga aagcgcacct ggaactcggc ccgctgctgg tgccggtaca
     4861 gcatcttcag aagcgtgcgg ctctcctgac cggacagctc gttgatccgc gttgtgaagc
     4921 taggattcac gtagagcgcc tggcggccgc tcctcggatg cgtgcgcacg acgggatgaa
     4981 cggcgctggg gtaggttctg ccggcatcct cgacgccccg gtcctcatag cggccgcggt
     5041 agacgtgctc cgaagcgtga gttgccgtga gcccggcgag aaactgccgc atcgccgcag
     5101 acagggtctc gtacgcgccg tacatgctgg cgaacagcgt gtcgccgccg gtggccggca
     5161 gcacgtgaag ctgcagcatc gttgccatgg gcggttgctc gtcgcaggac acgtcagtgt
     5221 gccagccgtt gccgttggca accttggagt tgcgatcggt atggatgacg aaaatctccg
     5281 ggtagccctc cagatgaggg gctgcggggt gaatgtgcag gtcgccgaag cggcgggcga
     5341 acgcgatctg gacatcaggc tcgagacgcg gctgatcccg gaagaagagc accaggtgct
     5401 cggtgagcgc ccgatcgatg tccgcgaagg tctggtcgcc gagatcggtg agatccgctc
     5461 ccgagacctc cgcgccgatc acgggtgtca ggggcctgac ggtgatggtc aattggacac
     5521 ctcagacgag aagccggtag ggcgcctcca gcagatcccg aaccgttgcc agaaactgag
     5581 ccgccggcgc gccgtccagc gcgcggtggt cccaggtcag cgacagccgc atggcctggc
     5641 gtttgacggg caccgcgccg tcccacgcca cgtcgtcgcg cagccggttg accccgagaa
     5701 tgcccacctg gggcgagttg atgatgggcg taaaggagtc gacgccgaac atgcccaggg
     5761 ccgagaccgt gaacgtgccg ccctgaagat cgtcgggcgt gaggttgccg tcccggcatg
     5821 cggccgccag ccggctcgat tcccgggcga gctctttcat gttgagcacg ttggcgtggc
     5881 ggacgacggg cacgatcagc ccttcctcta aagccacggc catgcccacg ttgacctcgc
     5941 ccttgagcgt catctccgtc tcgccgaaca cgctgttcat gagcggatgc ttttccaggg
     6001 ctttggcggc ggcacggatc accaggtcgg tgtacgtcgg gcgcacgcct tcgtcggccc
     6061 actcctcgat gagctggccg cgcagcttga ccgcgtcgtc catgatcacg tccatgtcca
     6121 tggtcagctg ggccatggac tggagactct cgtgcatgcg ggcggcaatg gtcttgcgca
     6181 tgccgcggac gggcacggtc tggtcgccac gcgaggccac cgcctccctg gccgccgggg
     6241 cggccgccgg gggtgccttg gcggccagtt ccacgtcttc cttggtgatg cgccctccgg
     6301 gccccgtccc ggtgaccgtg gcgatgtcca caccgagctc ccgggccacc ttgcgcgcca
     6361 tgggcgacga tctgctgccg cgctcttcgc ccgcgcccgc agctgctgcc tgaacgtctg
     6421 cctcgacgat ccgcccgccc ggaccggtcc cggccagggt cgagatgtcg acgccgagct
     6481 cgccggccag cctcctggcg gctggcgagg acaggatgcg ctccccatcg ccccgagtta
     6541 tcgcggccgg tgctgcggcc gcgg
//
LOCUS       DBNHKV010000344         2927 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_261075, whole genome shotgun sequence.
ACCESSION   DBNHKV010000344 DBNHKV010000000
VERSION     DBNHKV010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_261075"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="AC16PP_10990"
     CDS             complement(<1..498)
                     /locus_tag="AC16PP_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6482197.1"
                     /translation="MALAKRMAVTLPAGPKIEHTVARLQWAEDNGFADAWFGDSGAPD
                     SLTQVAAVAHHTSSIRIGVAVTPVYTRTPAVLAATANVLEQVLPGRFVMGLGSSSQTI
                     MGQWNGIPLDRPLTRVRETAILVRSMLTGEKSNFDGETLTSHGYRQPPLDHPPPIYLA
                     ALRPKM"
     gene            600..1643
                     /locus_tag="AC16PP_10995"
     CDS             600..1643
                     /locus_tag="AC16PP_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6482198.1"
                     /translation="MSDVELTPVREMHRFDEDALDRYLQAHVNGYRGPLTVRQFEGGQ
                     SNPTFQLEAGGRLYVLRKQPPGELLPSAHQVDREYRVMKALEQTEVPVPLMKCLCEDA
                     GVIGTKFYVMEMVNGRVFAETLLPSLTPPERRAIYLDLARVLAKLHSVDPAAVGLADF
                     GRPGNYYERQIGRWTKQYEASKTEQIDAMDRLMAWLPERIPEPGETVIVHGDYRLGNA
                     LIHPTQPRIVAVLDWELSTLGDALADLGYICQDYHGDSYNDAGLSGADLGALGIPTED
                     ELVAEYCRHAGREHIDNWPFYVIYNMFRSASIIQGVYKRGLDGNASSEKALEYKDAAR
                     IRSEQAWALVESL"
     gene            complement(1645..2685)
                     /locus_tag="AC16PP_11000"
     CDS             complement(1645..2685)
                     /locus_tag="AC16PP_11000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6482199.1"
                     /translation="MKLGILSGYSGRKMNIPIDAIRHAESLGYESIWTAEAYGSDAVT
                     PAAWILAQTQKIKVGTAIMQMPARTPAMTAMTAMSLAELSGGRFICGVGASGPQVVEG
                     WHGVPYGLPVTRLKEYVQIMKRIFAREAPLEFEGRMYQLPYQGPGATGLGKPLKSILH
                     CEEDIPIYAATITPKGVQAAAEVADGFFPVWMDPEQYHVFEEPVAKGFASAGGDKDLT
                     RFDIAPFVTVVMGDDVEQCMMPIRGSMALYIGGMGARGKNFYNDYAKRLGFEEAAVRI
                     QDLYLEGRKDEAMAAVPAELIDACHLVGPADRIRERLQRWQAAGRQGHVSSMLLGCQQ
                     PEALALVAETVL"
BASE COUNT          518 a          979 c          939 g          491 t
ORIGIN      
        1 catcttcggg cgcagggcgg ccagatagat gggcggcgga tggtcgagcg gtggctggcg
       61 gtaaccgtgg ctcgtcaacg tctcgccgtc gaagttgctc ttctcgccgg tcagcatgga
      121 acgcaccagg attgccgtct cccggacccg ggtcaggggc cgatccagcg gaatcccgtt
      181 ccattgaccc atgatggtct ggctcgagga gcccaggccc atgacgaaac gtccgggcag
      241 gacctgctct agcacgttgg ccgtcgcggc gagcaccgca ggcgtgcgcg tatacaccgg
      301 cgtgacggcc acgccgatgc gtatgctgct ggtgtgatgg gcaaccgccg ccacctgagt
      361 gaggctgtcc ggcgcgcccg agtcgccgaa ccaggcatcc gcgaaaccgt tgtcctcggc
      421 ccactgcaag cgcgcgacgg tgtgctcgat tttgggtccg gcgggcaagg tgaccgccat
      481 tctcttcgcc aatgccatgc tgcctcccca ggtgcggcct tgcggaaaac ccacagcctc
      541 gcattttgcg gccgacgaga tcaagccgta tccttgcccg tcgcttcggg agcaaagcaa
      601 tgagcgatgt agagctgacg ccggtgcgcg agatgcaccg attcgacgag gacgcgctgg
      661 accgttacct gcaggcccac gtgaacggct accgcgggcc cctgacggtg cgtcagttcg
      721 agggcggaca gtcgaacccg acctttcagc tcgaagcggg tggcaggctc tacgttctgc
      781 gcaagcagcc cccgggtgag ctgctgccgt cggcccacca ggtggaccgc gagtaccggg
      841 tgatgaaagc gctggagcag accgaagtgc ccgttcccct gatgaagtgc ctgtgcgagg
      901 acgcaggcgt gatcggcacg aagttctacg tgatggagat ggtgaacgga cgcgtgttcg
      961 cagagacgct gcttccgtct ctgaccccgc ccgaacggcg cgccatctac ttagacctcg
     1021 cccgagttct ggcaaagctc cacagcgtgg accccgcagc ggtggggctc gccgacttcg
     1081 gccgccccgg caactactac gagcggcaga tcggtcgctg gaccaaacag tacgaggcat
     1141 cgaagaccga gcagatcgac gccatggaca ggctcatggc gtggcttccc gaacgcatcc
     1201 cggaaccggg ggagaccgtc atcgtgcacg gcgactaccg gctgggcaac gcgctgatcc
     1261 accctacgca accccgcatc gtggcggttc tggattggga actgtcgacg ctgggggacg
     1321 cgctcgccga tctcggctac atctgccagg actaccacgg cgattcctac aacgatgcgg
     1381 gtctttccgg cgccgacctg ggcgcgctcg gcatccccac cgaggacgag ctcgtggccg
     1441 agtactgccg gcacgcgggc cgcgagcaca tcgacaactg gccgttttac gtgatctaca
     1501 acatgttccg ctcggcgagc atcattcagg gcgtgtacaa gcgcggcctg gacggcaacg
     1561 ccagctccga gaaagcgctg gagtacaagg acgcggcccg catccgatcg gagcaggcct
     1621 gggccctggt ggaatcgctc taagctagag gacggtttcc gccaccagcg ccagcgcctc
     1681 cggctgctgg caacccagca gcatgctgga aacatgcccc tgacgccccg ccgcctgcca
     1741 gcgctgcaac cgctcgcgaa tccgatccgc cggccccacc aggtgacagg cgtcgatgag
     1801 ctccgcgggc accgcggcca tggcttcgtc cttgcgccct tcgaggtaca ggtcctggat
     1861 cctgaccgca gcctcctcga aacccagccg cttggcgtag tcgttgtaga agttcttgcc
     1921 gcgggcgccc atgccgccga tgtagagcgc catgctgccg cgtatgggca tcatgcactg
     1981 ctcgacgtcg tcgcccatca ccacggtgac gaagggtgcg atgtcgaacc gggtcagatc
     2041 cttgtccccg ccagcgctgg cgaagccctt ggcaaccggc tcttcgaaga cgtggtactg
     2101 ctcgggatcc atccacacgg ggaagaagcc gtccgccacc tccgcggctg cctgcacgcc
     2161 tttgggcgtt atggtcgcgg cgtagatggg gatgtcctcc tcgcagtgaa gaatgctctt
     2221 cagaggtttg cccagacccg tggcacccgg accctgataa ggcagctgat acatccgccc
     2281 ctcgaactcg agcggcgcct cccgggcaaa gatgcgcttc atgatctgca cgtattcctt
     2341 caggcgcgtc accggaagcc cgtagggaac gccgtgccag ccttccacga cctgggggcc
     2401 ggaggcaccg acgccgcaaa tgaaacgacc gcccgacagc tcggcaaggc tcatcgccgt
     2461 catcgccgtc atggcaggcg tgcgtgccgg catctgcatg atggccgtgc ccaccttgat
     2521 cttctgcgtc tgcgccagga tccaggccgc cggcgtcacg gcgtccgagc cgtaggcctc
     2581 ggcggtccag atggattcgt agcctagact ctcggcgtgt ctgatcgcgt cgatgggaat
     2641 gttcatcttg cgtccggaat agccggaaag gattccgagt ttcatatgag gagcccccag
     2701 gtgtgtagtt tctggcggga ccgcgcccgc cgaaggcgca gcaagttaac agaccgccca
     2761 acccacggca acgcgagccc cgagaccgct cagccggctg cgaagcgttc ccgcaggtag
     2821 gtgatgatgt ccgcgctctc gtagagccag cgggtgcctt cctcggattg aatcttcagg
     2881 cagggaacca tggaccgccc gccgccgcga atcagctcgc ggagcgc
//
LOCUS       DBNHKV010000345         5369 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1960757, whole genome shotgun sequence.
ACCESSION   DBNHKV010000345 DBNHKV010000000
VERSION     DBNHKV010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1960757"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1410)
                     /locus_tag="AC16PP_11005"
     CDS             complement(172..1410)
                     /locus_tag="AC16PP_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HIR6482200.1"
                     /translation="MQAILGIGFLLGLAWILSESRRTVRWRPVMVGLVVQLGLAALLL
                     RVPVVAESLLVLNKLVYAVEAATGAGAGFVFGYLGGGDAPFTVSAQGAMYVFAFRVLP
                     QILVFSVLVALLWYWRVLPAVIRGFGYLLRRALDVGGAVGTAAASSVFLGMVEAPLVI
                     RAYLERLSRSELFTVMTCGMSTVAGSIMVLYASVLGDVITGALGHILAASVINVVGAV
                     VVSRIMIPADDATAEEDGASGLSYVSNMDAITRGTADGLRLAVNVGAMLVVLVSLIAL
                     VNQGLGAVTVGEGPLSLERMMGWVFSPVAWLIGIPWGEAQAAGALLGTKLILNELVAY
                     LQLAGLPEGSLSDSSRLVMTYALCGFANFGSLGILLGGLNTLVPERRKEILSIGPKTL
                     ISGTIVTCNTGAIVSLIGLF"
     gene            complement(1437..2129)
                     /locus_tag="AC16PP_11010"
     CDS             complement(1437..2129)
                     /locus_tag="AC16PP_11010"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase family
                     protein"
                     /protein_id="HIR6482201.1"
                     /translation="MLHHKSAMQGEPGQALAAELDIRIPRMDGLSLRVAPVADFEHEL
                     HGPELEAVARAVPKRRQEFATGRHLARAAMRELGLPAAPVPRRADRSPAWPDGCLGSI
                     THPNALAGVAVARAGVVEGIGIDLEESHRVSAELHDKLFTPTELGRYVDAPPGWADLL
                     FSAKESVYKAVNPIVGKYIGFHEVEVDVTFAEQRFSARYVGEHAPNALLENGAGYFLF
                     AEQHVFSVFIIP"
     gene            2132..2776
                     /locus_tag="AC16PP_11015"
     CDS             2132..2776
                     /locus_tag="AC16PP_11015"
                     /inference="COORDINATES: protein motif:HMM:NF019544.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol phosphotransferase CPT family
                     protein"
                     /protein_id="HIR6482202.1"
                     /translation="MPIPSPRMQTGKIIFLNGSSSAGKTTIAVTLQQLLEEPFQHIAL
                     DQFRDGMPGRFRGLNSPEGTPGARGLNVVPVERDGEKMTEIRFGEVGEQILRGMRRAI
                     SAFAAAGNNVIVDDLLFKPDYLHDYAKALADHDAWLIGVRCSLDVVNEREAQRPGRFP
                     GTATSHFHQVHEHGAQYDLEVDTTTTTPRACAELIIERLARPPEALERLRSARP"
     gene            2843..3601
                     /locus_tag="AC16PP_11020"
     CDS             2843..3601
                     /locus_tag="AC16PP_11020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR6482203.1"
                     /translation="MTENEDVDAFRELIEAAGNVVFFTGAGISTESGIPDFRSPGTGL
                     WNKIKPIEFQDFVASEDKRREYWQRHFTGERPMANAKPNKGHDGVQRLIASGKATAVI
                     TQNVDNLHQDSGIPEDQVIELHGNASYATCLACGTRYELADLEQQFHRHGNVEPCGRC
                     GGIVKSATISFGQAMPDAAMLRAQAATEACDLMIVIGSSLVVYPAAGFPEYAKRRGAG
                     LVILNREETPLDPFADLVLHRDIGPTMSYVTGIN"
     gene            3959..4795
                     /locus_tag="AC16PP_11025"
     CDS             3959..4795
                     /locus_tag="AC16PP_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6482204.1"
                     /translation="MPGEEPDIGEIRRGICRLLPEWAPEELLDFDYLPAGYSNHNFRF
                     ARRGNQYVVRVPLESPGEQALAGERSVYEALTDPSVPAVIALGDDGWLVSRWVEGPLL
                     VDAPPSAQTIVGFIRRLHESLRVRAAPYDPAARSRAYLETPGDSSPDARVMAIAHSLN
                     WRLDDTVACHNDLNPWNVIVSASGDWVTLDWQSVGANDPLFDLVTLHQGLSLPDAGLT
                     DLCEALLERPVAPERVERVLVGFWLREYAWAFAALQLGNRRQEILDQLALSRDRLASL
                     AG"
     gene            complement(4797..>5369)
                     /locus_tag="AC16PP_11030"
     CDS             complement(4797..>5369)
                     /locus_tag="AC16PP_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6482205.1"
                     /translation="GNGHHGLAGVATEFEPGLVVCLTGPESTGKSTIAEQLASALAVP
                     LVPEVAREYLAGRPDYEQSDLLAIARAQLAAEQDALAEGTGLVVCDTDLQVIQIWWEE
                     RYAELDPWLAQELVDRSARRYLLAVPDLPWEPDPLRESPHDRERLFRRYRQVLREGAF
                     PYAEVRGLGRSRFDSALVTVQRWLAEPQQD"
BASE COUNT          984 a         1835 c         1718 g          832 t
ORIGIN      
        1 gttcctgaac ctcgccgagg cggaagtgga taggctcaag gaccatctgg ccgatcgtgg
       61 catcgtcatc cgcgggccgc gctgggtgac ccatctcgac gtctccgacg aggacgtcgc
      121 gcgcgtcgtc gacgccattc gggcaagctg aggcgccttg gtcttaccgc ttcagaacag
      181 gccgatgagc gacacgattg ccccggtgtt gcaggtaaca atcgtaccgg aaatcaacgt
      241 cttaggtccg atacttaata tctctttgcg tcgttccgga acgagcgtat tcaggccccc
      301 gagcagaatg cccaggctgc cgaaattggc aaagccgcac agcgcgtagg tcatcaccag
      361 ccggctcgag tcgctcaagc ttccctccgg cagcccggcg agctgcagat aggccaccag
      421 ctcgttcagg atcagcttgg tgccgagcag cgccccggcc gcctgggctt ctccccaggg
      481 aatcccgatc agccaggcta cgggggagaa cacccatccc atcatccgct ccagagaaag
      541 cgggccctcc cccaccgtca ccgcgcccag gccctgattc accagagcga tcaggctcac
      601 gagaaccacg agcatggcgc cgacgttcac ggccagacgc agaccgtccg ccgtgccgcg
      661 cgtgatggcg tccatgttgg agacgtagct gagaccggaa gcgccgtcct cttctgccgt
      721 ggcgtcgtcc gccggaatca tgatgcgcga gacgacgacg gcgccgacca cgttgatcac
      781 cgaagcggcc aggatgtggc cgagcgcgcc cgtaatgacg tcgccaagca cgctggcgta
      841 gaggaccatg atggatccgg ccacggtgga catgccgcag gtcatgaccg tgaagagctc
      901 cgaacgggac aagcgctcga gataggcccg gatcaccagc ggtgcttcca ccatgccgag
      961 aaacacgctg gaggcagccg ccgttcccac ggcgccgccc acatcgagtg cacgacggag
     1021 caggtagccg aacccgcgga tcaccgccgg cagcacgcgc cagtaccaca gcagcgccac
     1081 gagcacgctg aaaacgagga tctgcggcag aacgcgaaac gcaaagacgt acatggcccc
     1141 ttgagcgctt accgtgaacg gcgcatcccc accgcccaga taaccgaaca cgaaccccgc
     1201 cccggccccg gtcgccgcct ccaccgcata gacgagcttg ttgagcacca gcagagattc
     1261 ggccaccacg ggaacccgca gcagcagcgc ggcaaggccg agctgaacga cgagacccac
     1321 catcacgggc cgccagcgca cggtgcgccg gctctcgctc agaatccacg ctagccccag
     1381 cagaaagccg attccgagta tggcctgcat tcagactctg ccttcgaggt aggggttcac
     1441 ggaatgatga acacgctgaa gacgtgctgc tcggcgaaaa ggaagtagcc cgcgccgttt
     1501 tcgagcaagg cgttgggagc gtgctcgccc acgtagcggg cagaaaagcg ctgctctgcg
     1561 aacgtcacat ccacctccac ctcgtgaaac ccgatgtact tgccgacgat cgggttgacc
     1621 gccttgtaga cggattcttt ggcgctgaac agaagatcgg cccaccccgg cggcgcatcc
     1681 acgtaccggc cgagctctgt cggggtgaag agcttgtcgt gcagctcggc agacacccgg
     1741 tgcgattcct cgagatcgat gccgatgcct tccacgacgc cggctcgggc aacggcgacg
     1801 ccggccagcg cattggggtg ggtgatgctg ccaaggcacc cgtcgggcca tgcggggctg
     1861 cggtcggccc ggcgcgggac cggggcggct ggcaggccga gctcgcgcat ggccgcacgg
     1921 gccagatgac ggccggtggc gaattcctgc cgccgctttg gcacggcccg ggcaacagcc
     1981 tctagctccg ggccatggag ctcgtgctcg aaatccgcca cgggggcgac gcgcaggctc
     2041 aggccatcca tccgcgggat gcggatgtcg agctcggccg ccaacgcttg tccaggctct
     2101 ccctgcatgg ccgacttgtg gtgcagcatg ggtgccgatc ccatcgccgc gtatgcagac
     2161 cggcaagatc atctttctga acggctcttc gagcgctggt aagaccacca tcgccgtcac
     2221 gctccagcag ctgctcgagg agccctttca gcacatcgcc ctggaccagt tccgcgacgg
     2281 gatgccgggc cgtttccgcg ggctgaattc ccccgaggga acgcccgggg cccggggcct
     2341 gaacgtcgtt ccggtagagc gcgacgggga gaagatgacc gagatccgct tcggcgaggt
     2401 gggtgagcag atcttgagag gcatgcgccg agcgatctcc gcctttgccg ccgctggtaa
     2461 caacgtgatc gttgacgatc tgctcttcaa gcccgactac ctgcacgact acgcgaaggc
     2521 cctggcagat cacgacgcct ggctgatcgg cgtgcgctgc agcctcgacg tggtcaacga
     2581 acgcgaagcg cagcgccccg ggcgctttcc cggcaccgcc acctcgcact ttcaccaggt
     2641 gcacgagcac ggcgcgcagt acgacctgga ggtcgacacc acgacgacga ccccgcgcgc
     2701 gtgcgcggag ctcatcatcg aacgcctggc ccggccgccg gaagcgctcg agcgactgcg
     2761 ctcggccaga ccctgatcct gggacatcgg caatcgacaa cgcggggctg aacacgcgac
     2821 aaccccacag ggaggcaggc caatgaccga gaacgaggat gtcgacgcgt ttcgcgagct
     2881 gatcgaagcc gctgggaacg tggtgttctt cacgggcgcg ggtatcagca cggagtccgg
     2941 cattcccgac tttcgcagcc ccgggaccgg actgtggaac aagatcaagc ccatcgagtt
     3001 ccaggacttc gtcgccagcg aagacaaacg ccgggagtac tggcaacgcc acttcaccgg
     3061 cgagaggccc atggccaacg ccaaaccgaa caagggccac gacggggttc agcggctgat
     3121 cgcctcgggc aaggcgaccg ccgtgatcac ccagaacgtg gacaatctgc atcaggactc
     3181 gggcattccg gaggatcagg tcatcgagct gcacggcaac gccagctacg ccacctgcct
     3241 ggcctgcgga acccgttacg agcttgccga tctggagcag cagtttcacc ggcacggtaa
     3301 cgtcgagccc tgcggtcgct gcggcggcat cgtcaagtcc gcgaccattt ccttcggcca
     3361 ggccatgccc gatgcggcca tgctccgggc ccaggcggca acggaagcct gcgacctgat
     3421 gatcgtgatc ggctcttccc tggtcgtcta cccggcagcc ggtttcccgg aatacgcgaa
     3481 acgacggggc gcgggtctcg tcatcctgaa ccgggaagaa accccgctgg acccgttcgc
     3541 agatctggtt ctccaccggg atatcggccc cacgatgtcc tacgtcacgg gcatcaacta
     3601 gccggtcagg ctgagcagac ggtcggcgaa gcgctcgatg tccttgagcc gggcgcacgg
     3661 ctgcttcgcc gccggatccg gaggcgagct ggcgaaccaa gcgctcgaag cagtcggcaa
     3721 agcgcgcttt ctcttcgcgg gtcttggcaa gggctagccc cagggcgtcc tgaagcagaa
     3781 ccggatcggc aatacccacc tgacgcacca cggcgaaccc gtccagggtc tccgcgggcg
     3841 aaacctcgac ctcggcgctg cgcagggcgc gaacgaagcg gtttagggtg acctccatgg
     3901 gtgaccgatt ctagcaagcc gggctaccat ccgatggctg agtttaaggg cctcacgttt
     3961 gccgggcgaa gaaccagaca ttggcgagat ccgtcgcggc atctgccgcc tcctccccga
     4021 gtgggcgccg gaagagttgc tcgacttcga ctacctgccc gcgggttaca gcaatcacaa
     4081 cttccgcttc gcccgccggg gtaaccagta cgtggttcgg gttcccctcg aatcccccgg
     4141 cgagcaggcc ctggcgggcg aacgttccgt ctacgaggcg ctgacagacc cttcggtacc
     4201 ggccgtgatc gccctgggcg atgacggctg gctggtatcc cgctgggtcg aaggcccctt
     4261 actcgtcgac gccccgccgt cagcgcagac catcgtcggt ttcatcaggc ggctgcacga
     4321 atcgctccgc gtgagggcag cgccctacga tcctgcggcg cgcagccgtg cctatctcga
     4381 aacgcccgga gactcgtcac cggacgcccg ggtgatggcc atcgcccaca gccttaattg
     4441 gcgcctggac gacaccgtcg cctgccacaa cgatctcaac ccgtggaacg tcatcgtctc
     4501 ggccagcggt gactgggtga ccctcgactg gcagtccgtc ggcgccaacg acccgctgtt
     4561 cgatctggtg accctgcatc agggtttgag cctgccggac gccgggctga cggacctgtg
     4621 cgaagcgctg ctggagcggc ccgtcgcgcc cgagcgggtg gagcgagtgc tcgtgggatt
     4681 ctggctgcgc gagtacgcct gggcctttgc cgcccttcag cttggtaacc ggcgtcagga
     4741 aattctcgat cagctcgccc tgtcccgtga ccggctggct tctctggccg gctgattcaa
     4801 tcctgctggg gttcggccag ccagcgctgc acggtaacca gcgcggaatc gaaccgcgac
     4861 cgccccagac cgcggacttc ggcgtacgga aaagcgcctt ccctcaggac ttgccggtag
     4921 cgtcgaaaga gccgctcccg gtcgtggggc gactcgcgca gggggtcggg ctcccagggc
     4981 agatccggaa cggcgagcag gtagcgacgg gccgaacggt cgacgagctc ctgggccagc
     5041 cacgggtcca gctccgcgta gcgttcctcc caccagatct ggatgacctg caggtcggta
     5101 tcgcacacga cgagaccggt gccttcggcc agggcgtcct gctccgcggc gagctgcgcc
     5161 cgggcgatgg ccagcaggtc ggactgttcg tagtcgggtc ttccggcgag atactcccgc
     5221 gcgacctccg gcaccagcgg gacggccagg gcgctggcca attgctccgc gatggtgctc
     5281 ttgccggtgc tctcggggcc cgtcaggcag acgacgaggc caggttcaaa ctccgtcgcc
     5341 acgcccgcca gcccatgatg gccattgcc
//
LOCUS       DBNHKV010000346         6211 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_686922, whole genome shotgun sequence.
ACCESSION   DBNHKV010000346 DBNHKV010000000
VERSION     DBNHKV010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_686922"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="AC16PP_11035"
     CDS             complement(<1..520)
                     /locus_tag="AC16PP_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482206.1"
                     /translation="MRTLVIQSASELDRPGWLSSCLQSVEGWARERGYDYRFRGDDAL
                     ELTPGWYRAKLIGRSPIVADLARLLLIRDALAKGYERACWIDADVLLYAPERLDLPDD
                     DECAFGREYWVQKNRDGRWQVRRNVHNALCSFVAGCPVLPFLIHATQRIISRADPQHI
                     APQMVGPKLLSAL"
     gene            615..1565
                     /locus_tag="AC16PP_11040"
     CDS             615..1565
                     /locus_tag="AC16PP_11040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR6482207.1"
                     /translation="MDWQAKLNEGGIVLLDGGTGSELQRRGVPMSGAAWSGAAAHTHP
                     DVLRAVHHDYIAAGAEVIITNTFGTSRATLRAAGLGDAFASINRRAVALAMEARELAG
                     RDVAIGGSISNLPPNMNIGDYPDPAEELADLRDQAAILAEGGVDFIALEMMQDVNHAP
                     RAMRAAVATGLPVWLGVSCRRSTQTASLVAFDFADTPFEAPLDALIAMRPAVVNVMHS
                     EVEATVPAIEAVRERWPGPIGAYPEIGYFAAPDRRFGDDHPPDRLVSEARRWVAAGAR
                     LIGGCCGTTPDHIRALHAALPELERLAGETETPGRSPTVR"
     gene            complement(1588..2622)
                     /locus_tag="AC16PP_11045"
     CDS             complement(1588..2622)
                     /locus_tag="AC16PP_11045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR6482208.1"
                     /translation="MQVGLVTGKETVDLVEMPDPRPEPGKAVVDVSYCGICGTDVHAY
                     QSGEPYNPAICGHEWMGHVSAAGAGVQVKEGDRVAIGMATACGQCATCVRGDPSHCEM
                     AFAGMVGIGPMAAPHGGFASAIAIEAARLYHVGPEISDEAAAILEPVTIAVHAVRRTG
                     IRLGDSVVVVGAGPIGLLVLQCARAAGAGVCVLVEPQATRRETGAALGADHTIDPRET
                     PDVAAAINSIIGKSGADVVFECAGLARTVEEVPAYVRRGGTVALVGYPTGLSQISAAQ
                     WLAQELKVSTALGYVRDDFEVSKGLVADGRVDCGPLHTSTVSLAGMAGAFAELASEPE
                     QIKILVDPRA"
     gene            complement(2692..3483)
                     /locus_tag="AC16PP_11050"
     CDS             complement(2692..3483)
                     /locus_tag="AC16PP_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR6482209.1"
                     /translation="MARLISLASGTLPEFGPAEVTRAAAAGGFNGCGIWFDAATWTDE
                     TTRGVAAAFADTGLTPLEIEVIDIRPGAPDPEHQRLLESGAAVGATEAIVVSSDPDEV
                     AVCRRFAHLCELGEQLGVNVCLEFLPIYAVRDLPCALRIIRDVGHPRGKLLIDPLHVA
                     RSGASVADIAAVPGELLSFAQFCDGAKEPPGEPTLDVLREEAVNGRFTPGAGQLPLRD
                     LLDVLPDDLPLSLELRSRPLRERFPDPAERARHVYRETARFLGGA"
     gene            3763..4533
                     /locus_tag="AC16PP_11055"
     CDS             3763..4533
                     /locus_tag="AC16PP_11055"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR6482210.1"
                     /translation="MRLVSWNVNGLRACAKKGFLGFLETSGADFVALQEVRAFPDQLD
                     DGLRAPPGWHVCFSPAERPGYSGVALYARAAPDRVQTSLGKPDFDREGRLLSAHFGRL
                     AIVSVYFPKGSGKDRDNSRVGYKLDFYAAVFDHVEQLRRRGPVFVVGDYNTAHEPIDL
                     ARPKTNTKSSGFLPEERAEITRWIDAGWVDVFRRQHPGQPDHYTWWRQWGNARRDNVG
                     WRIDYVLASPSAARRVRQAFIWPEVTGSDHCPVGVDFE"
     gene            4737..5042
                     /locus_tag="AC16PP_11060"
     CDS             4737..5042
                     /locus_tag="AC16PP_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482211.1"
                     /translation="MCRHHDESASAGRCREERADPPPEKDVANFCDWFQPGKNAFQEG
                     RTTAGENARSRLDALFGDSQGPAEEPDEAPPKPEDGPRSAEEKARAELDRLFSRDAD"
     gene            5404..>6211
                     /locus_tag="AC16PP_11065"
     CDS             5404..>6211
                     /locus_tag="AC16PP_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6482212.1"
                     /translation="MSLVDALEYAAQGDTGLNFYDGSGSLSAALPYAQLAQDARILAT
                     RLLGLGVKRGARVGIVAETDPMFPRFFFACQYAGLIPVALPAGLQMGGGEAYVEQLSR
                     MLESCGAAVAVAPESHLSFLKRATRQLSLRMSGSPEDFDALPAVPVKLLPLSADEPAY
                     LQYTSGSTRFPRGVEMTQTAVLANLTEIAEIGTRITADDRIVSWLPYYHDMGLVGCLL
                     VPVATQISADFLSPRTFAMRPRLWLKLLSENRGTISSSPPFGYELCATRVR"
BASE COUNT         1081 a         2088 c         2036 g         1006 t
ORIGIN      
        1 gcagggcgga aagcagcttg gggcccacca tctgcggggc gatgtgctgg ggatccgcac
       61 gggagatgat cctctgggtc gcgtgaatca ggaagggcag aacggggcag ccggcaacga
      121 aggagcacaa ggcgttgtgc acgttgcgac ggacctgcca ccgcccatcg cgattcttct
      181 ggacccagta ctcccggccg aaggcgcatt cgtcgtcatc gggcaggtcg aggcgttccg
      241 gtgcgtacag gagcacgtcg gcatctatcc agcaggcacg ctcatagccc ttggccaacg
      301 cgtcgcgaat cagcagcagc ctcgccaggt ccgcgacgat gggagagcgc ccgatgagct
      361 tggcgcgata ccaccccggc gtgagctcca gcgcatcgtc gccgcggaag cgatagtcgt
      421 agccacgctc ccgcgcccag ccttccacgc tttggaggca cgacgacagc caacccggcc
      481 ggtccagctc gctggcgctc tggatgacaa gtgtccgcac gcgtatcagt tgcgcacgcg
      541 gccgatgggt gctgccggca cggaaacgct cacgtcagct aaaatccttt cgtctcatta
      601 gaaacgtgaa gcgagtggac tggcaagcga agctcaacga gggcggcatc gtcctgctgg
      661 acggaggcac cggctccgag ctccagcgca ggggcgtgcc catgagcggc gcagcctggt
      721 cgggggcggc cgcgcacacc catcccgacg tgttgcgcgc cgtgcatcac gactacattg
      781 cagccggcgc tgaggtgatc atcaccaaca ccttcggaac atcccgggcc acccttcgag
      841 ccgccggcct gggcgatgcg ttcgcgtcca tcaaccggcg cgccgtggcc ctggccatgg
      901 aagcgagaga gctcgccggt cgcgacgtcg ccatcggcgg gtcgatatcc aacctcccgc
      961 ccaacatgaa catcggggac tatcccgacc cggccgagga actggccgat ctgcgcgatc
     1021 aggctgccat cctggcggag ggcggcgtcg atttcatcgc cctggagatg atgcaggacg
     1081 tgaaccacgc gccaagagcg atgcgcgccg cggtggcaac cgggcttccc gtctggttgg
     1141 gcgtcagctg ccggagatcg acgcaaaccg cgagcctcgt ggctttcgac ttcgctgaca
     1201 cgccgttcga agcgccgctg gacgcgctga tcgccatgcg tcctgcggtg gtcaacgtca
     1261 tgcacagcga ggtcgaggcg accgtgcccg ccatcgaggc ggtccgcgaa agatggcccg
     1321 gccccatcgg cgcctatccg gagatcggct acttcgcggc ccccgaccgg cgcttcggcg
     1381 acgatcaccc gccggaccgt ctggtgtccg aggctcgcag gtgggtggcg gcgggcgctc
     1441 gattgattgg cggctgctgc ggaaccacac cggatcacat ccgggcgttg cacgctgcgc
     1501 tgcctgagct cgaaaggctc gccggggaga ccgagacgcc gggccgttcg cccaccgtcc
     1561 ggtgatggtc tctccaacgc agaacggtca ggcgcggggg tcgaccagaa tcttgatctg
     1621 ctccggctcg ctggcgagct cggcaaaggc tcccgccatg cctgcgaggc tgaccgtcga
     1681 ggtatgcagg ggaccgcagt cgacgcggcc gtccgcgacg aggcccttgg aaacctcgaa
     1741 gtcgtcccgc acgtagccga gcgccgtgga taccttgagc tcctgtgcga gccattgcgc
     1801 cgcggaaatc tgcgacaacc cggtggggta acccaccagc gccacggtgc cgccccggcg
     1861 aacgtaggcg gggacttcct ccacggtgcg cgccagcccc gcacactcga acacgacgtc
     1921 ggcgcccgac ttgccgatga tcgagttgat ggcagccgcc acgtcggggg tctcccgggg
     1981 atcgatggtg tggtcggcgc ccagcgctgc cccggtttcg cgccgggtag cctggggttc
     2041 caccagcacg cagacacccg cgccggcggc ccgggcacac tgcagcacca gcaggccgat
     2101 ggggccggca cccaccacca ccacgctgtc gccgagccgg ataccggtgc ggcggaccgc
     2161 atgaacggca atggtgaccg gctcgagaat ggcagcggcc tcgtcgctta tttccgggcc
     2221 tacgtggtag agacgagccg cctcgatggc aatcgccgag gcgaagcccc cgtggggggc
     2281 cgccattggg ccgattccga ccatgccggc aaaggccatc tcgcagtggg acgggtctcc
     2341 gcgcacgcag gtggcgcact ggccacaggc ggttgccatg ccgatggcca cgcgatcgcc
     2401 ttccttgacc tgcacgccgg cgcctgccgc cgagacatgg cccatccact cgtgcccgca
     2461 gatggccggg ttgtaaggct cgcccgactg ataggcatga acgtccgtcc cgcagatgcc
     2521 gcaataggaa acgtccacca ccgccttgcc cggctccggg cggggatcgg gcatctccac
     2581 gagatcgacc gtttccttac ccgtaactaa cccaacctgc atggccttac ccccgtttcg
     2641 aattgcgcca aaggatacgc cagtcggcgc gcagcggcat ccttggacgg ttcaggcgcc
     2701 gccgaggaac cgggccgttt cgcgatatac gtgtcgggcg cgctccgcgg gatccgggaa
     2761 acgctcgcgc aggggtcggg agcgcagctc caggctgagt ggcaggtcgt cgggcaggac
     2821 gtccagaaga tcgcgcaggg gcagttgacc ggcccccggg gtgaaccggc cgttgacggc
     2881 ctcctcccga agaacgtcca gggtaggctc gcctggaggc tctttggccc catcgcagaa
     2941 ctgagcaaag ctgaggagct cgccgggcac agcggcgatg tccgcaacgc tggcgcccga
     3001 cctggcgacg tgcagaggat caatgaggag cttgccgcgc ggatgtccca cgtcccggat
     3061 gatccgcagg gcgcaaggca gatcccgaac cgcgtagatc ggcagaaatt cgaggcagac
     3121 gttgacgccc agctgctcgc caagctcgca gagatgggcg aatcggcggc agacggccac
     3181 ctcgtcggga tcgctcgaga ccacgatggc ctcggtggcc ccaaccgcag cgccgctctc
     3241 cagcaaacgc tgatgttccg ggtccggtgc tcccggccgg atgtcgatga cctcgatttc
     3301 cagcggcgtc agaccggtgt ccgcaaaggc ggccgccacg ccgcgcgtgg tctcatcggt
     3361 ccacgtggcg gcgtcgaacc agataccgca gccgttgaac ccccctgcgg cagccgcccg
     3421 ggtgacctcg gcaggaccga actcgggcag ggttcccgat gctagagata tgagacgcgc
     3481 cacgatgatc gtcctgattg ctgcctgggc cagtgtgctt catttgcagc gttctggcga
     3541 gcaggcccgc taccccatag ctcccgcggc cgggccgccg tcaggcactg ggggcgactc
     3601 gacgggaagg caggactcct ccgggcgcag gctccccggg cgctcaactc ggatctgcac
     3661 atcttccccg gggcgatttg ccgacgcggc gtggaacgtc gcgggccgga tgttaaagtg
     3721 cgccccgccc gcgcgccctc aaacaggagc gagactggat cgatgcgact tgtcagctgg
     3781 aacgtcaacg gcctgcgcgc ctgtgcgaaa aagggctttc tcggatttct cgagacctcc
     3841 ggggccgact tcgtcgccct gcaggaagtg cgcgcgttcc ccgatcagct ggacgacggc
     3901 ctgcgagccc cgcccggctg gcacgtttgt ttctctcccg cggagcgccc cggctacagc
     3961 ggcgtcgccc tctacgcccg ggccgcccct gatagggttc agaccagcct cgggaagccg
     4021 gacttcgatc gggaggggcg gctgctcagc gcgcatttcg gccgcctggc catcgtgagc
     4081 gtctacttcc ccaaaggcag cggcaaggat cgggacaaca gccgcgtcgg ctacaagctc
     4141 gatttttacg ccgccgtgtt cgatcacgtt gagcagctgc gccggagagg tcccgtattt
     4201 gtggttggcg actacaacac ggcccacgag cctatcgacc tcgcccgtcc aaaaaccaac
     4261 accaagtcga gcgggtttct gcccgaggag cgagccgaga tcacgcgctg gatcgacgcc
     4321 ggctgggtgg acgtcttccg ccgccagcat ccggggcagc ccgaccacta cacgtggtgg
     4381 cggcagtggg gcaacgcccg ccgggataac gtcggctggc gaatcgacta cgtgctggcc
     4441 tcgccatcgg ctgcaaggcg ggtcaggcag gcgttcatct ggcccgaggt gaccggatcc
     4501 gatcactgcc cggttggcgt tgacttcgaa tgagacgacg ccaccgggcc cgcattgacc
     4561 cgcattgcct cgagcgcatc ggatttctat agtccaccca tcgtccaggc cagaggcagt
     4621 ctcccgtggc agacgcacgt tgctggaatt gtggtacgtc gatcgacgac gtgcccagac
     4681 ccataagccg gcaagcaacc tgttctaagt gcttccacga actgcactgt tgtcgcatgt
     4741 gccggcacca cgatgagtcc gccagcgcgg gaaggtgccg ggaggaacgt gcagatcccc
     4801 ctcccgaaaa ggacgttgcc aacttttgtg actggtttca gccaggcaag aacgccttcc
     4861 aggaaggccg caccacggcg ggcgagaatg cccgctcacg cctcgatgcg ctgttcggcg
     4921 attcgcaggg cccggcggaa gagcctgacg aggcgccgcc aaaacccgag gatgggcccc
     4981 gaagcgccga ggagaaggcc cgcgccgagc tcgatcgcct gttttcgcgg gacgctgact
     5041 gattgcgccc cgcgtcggat atcgctccga ggcgagatgg gcccgaccag cgacatcgca
     5101 aatctgtgac attcacacgc catttccgtg aaacgcaccc gttatcgcgg gtctacaatc
     5161 cgtaggtctc tgatttcgtt tgaaaagaca ctatcgctct cagacgaatt tgcaggtaga
     5221 ctgcgccacc agaaatataa gtgccggatg attcgccggc cgatggagag cctcggtttg
     5281 ttgggcagct gtgttcggag gggttcgagc acaacgaaaa gagcaaacac gacaatttga
     5341 gctggttatc acatgagccc taccactcgt tgtagcggga tatcgcaccg cacacgtggc
     5401 tttatgagcc tggttgatgc cctggagtac gccgcgcaag gcgacacggg gctgaacttc
     5461 tatgatggct ccgggagcct cagcgccgcg ttgccgtacg cgcagctggc gcaggacgct
     5521 cggatcctgg cgacccggct gctcggcctg ggcgtcaaac gcggcgcccg ggttggcatc
     5581 gttgccgaaa ccgaccccat gttcccgcga ttcttctttg cctgtcagta cgccgggttg
     5641 atcccggtgg ctctgcctgc cggtctgcag atgggcggcg gcgaggccta cgtggagcag
     5701 ctcagtcgca tgctggaaag ctgcggagct gcggtggcgg tcgcgccgga atctcacctc
     5761 agcttcctga agcgtgcgac gcgacagctg tcgctgcgca tgtccggatc gccagaggac
     5821 ttcgacgccc tgccagcggt gccggtgaag ctgctcccct tgagtgccga cgaacccgcc
     5881 tacctgcagt acacgtcggg aagcacccgc ttcccccgcg gcgtcgagat gacccagacg
     5941 gccgtcctcg caaacctgac cgaaatcgcc gagataggca cccgcatcac cgccgacgac
     6001 cggatcgttt catggctgcc ctactaccat gatatgggcc tggtgggttg cctgctggta
     6061 ccggttgcaa cgcagatttc agccgacttc ctgtcgccgc gcacgtttgc catgcgtcca
     6121 cggctttggc tgaagctgct ctcggaaaat cgcgggacga tctcgtccag cccgccgttc
     6181 ggctacgagc tgtgcgcgac ccgcgtccgc a
//
LOCUS       DBNHKV010000347         5386 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1960955, whole genome shotgun sequence.
ACCESSION   DBNHKV010000347 DBNHKV010000000
VERSION     DBNHKV010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5386)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1960955"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="AC16PP_11070"
     CDS             complement(<1..1143)
                     /locus_tag="AC16PP_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6482213.1"
                     /translation="MSDPASYNLMEDETRECPFPYFNAIRHEQPVYFMEELGAWYVSR
                     YEDVRHVKKHPEIFSNNIFEHGGHRGAERNIAEEYKSEHGWRRVSTLQRTDPPVHSKY
                     RKLINHAFTVSRIRKMTGYVETVVNDLIDQFIDKGEVDLVWEFAIPLPCTVIADQLGV
                     PRDKIWRLKEWSDAMLAPGGGFVDEAAALKCGQLVVEAQKFFAEVMEARRAEPRDDII
                     TDLVQAKVVDEPGGPERPLDMFELQDLLDQLLTGGNETTTNAIGSAMMLLLQRPGLME
                     KMRDDQKLIRNFLEESLRFETPVLHLWRITTQDTDLGGAFIPKGSMVAIGYASANRDE
                     DVFQDSEVFDIERQKAGAHLAFGSGPHHCPGAALARQEMFSAFTILL"
     gene            1243..2106
                     /locus_tag="AC16PP_11075"
     CDS             1243..2106
                     /locus_tag="AC16PP_11075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6482214.1"
                     /translation="MKIGVFATFMSPLGTAEMIQDFGRRAEAAGLDSIWMGEHVVLFD
                     EMEFPYPGSRDGRIPVPEGGGLLDTVATFGLLAGSTSRLRFGTGICLVPQRNPIYTAK
                     EFATLDWLSGGRIDFGIGVGWCKEEVIACGYGWEDRGERCDEFLTLMRKLWSEPVVSY
                     SGKHVNVQGCRMDPKPIQKPHIPIIVGGHTPRAMRRAAEFGAGWYGFGITPDQTSAVL
                     TELDAALGAAGRSRDGFEIIITPASEDADSMAAFADLGVDRLILHLGSQRPEKLAARV
                     DQLASLVQAAA"
     gene            2192..3073
                     /locus_tag="AC16PP_11080"
     CDS             2192..3073
                     /locus_tag="AC16PP_11080"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6482215.1"
                     /translation="MARLAGKNIVVTGSSRGLGRAFAIAMAAEGASVVINGTNREALA
                     EVEKTISSAGGNVLAVPGSVTEETTCAQLVSGCVETFGSIDVMINNAGIVRDRTLLKM
                     TVEEWDDVLAVNLRGAFACTKHAALAMREAGGGHIVQVISASGLSGGFGQGNYAAAKA
                     GMMGLLRTAVLELSRFNIRNNALWPVAETDMTQVVFERAAAMSREQGAEPPTPAEMGF
                     GKPEEVAQGLVWLVSDGAAHFDGQCMSFNGRKTAIWRHPAEEHVSFHDEPWSFDELND
                     YYRDVKPLPIYRPVRAE"
     gene            complement(3083..3697)
                     /locus_tag="AC16PP_11085"
     CDS             complement(3083..3697)
                     /locus_tag="AC16PP_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482216.1"
                     /translation="MKAWALLLGIYGVFVYWYTSFQGPLSEAEIETYLAALREAGGND
                     ERLALWRAFMESDTGDDFVMINVVDLRDRPLAMEGVSPDETAADVLARYTAPFLGAAI
                     RSAAHPILYGNAAAPALDLWGIDGAERWSNGGLVRYRSRRDLMDQVVRTSRLDIHEFK
                     IAAMEKTIAYPLDPWWHLGDPRLLLALVLGCIGFGLQARGARGR"
     gene            complement(3723..5060)
                     /locus_tag="AC16PP_11090"
     CDS             complement(3723..5060)
                     /locus_tag="AC16PP_11090"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6482217.1"
                     /translation="MAAARSLPYPNRAYAWFVLAVLVVAALIAFIDRQVVAIVVDPMK
                     ADLGVGDAQIGWLYGVFALFYAVAALPIAWLADRKSRKHIIAAGIFLWSLMTIACGLS
                     RSYWNVFLARIGVGIGEATLTPATTSMVGDYFPRDQIPLALSIFQTGAIMGSGIAFII
                     GGVVLNIVEQAEPMVLPLVGELRPWQQTFVYVGAPGMILALLLLLLREPVRRSAGGTG
                     TQQEATFAEIVAFYRRNARTLACHHIGFLSFALMGYAFVFWTVSFFVRVHGYDAADAS
                     QVFGWIFFLAGPLGPILIALLARRLGERGRGDANITAGMIGGLISVPTILLIQFVPSP
                     MWAFVLYVPAMIFINSPFGIAAGSLPVITPPNMRAQVAALYMLTVSVGMMLGPPVAGA
                     FNEYVFPEAEGVRYSLITLTSLFGAIAGLSLWLGRRAYAVSLAEAEEQAAREG"
     gene            complement(5093..>5386)
                     /locus_tag="AC16PP_11095"
     CDS             complement(5093..>5386)
                     /locus_tag="AC16PP_11095"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6482218.1"
                     /translation="INALTKVVAYENGPDGITSNAILPGAVETDLMTITGAEAAKSLG
                     MTYEQYIDGYAQEAAIKRINTVEEVAAVALLLASDLGGGITGALMEVHGGTAL"
BASE COUNT          995 a         1774 c         1758 g          859 t
ORIGIN      
        1 cagcaggatg gtgaatgccg agaacatctc ctgcctggcc agggccgcgc cggggcagtg
       61 gtgcggaccg gagccgaacg ccagatgggc gcccgctttc tgacgctcga tgtcgaagac
      121 ctcggaatcc tggaaaacgt cctcgtcccg attcgctgaa gcgtaaccga tggccaccat
      181 ggatcctttg ggaataaacg ccccgccaag atcggtatcc tgggtggtga tccgccacag
      241 atggagcacg ggtgtctcaa agcgcagcga ctcttcgagg aagttgcgga taagcttctg
      301 gtcgtcccgc atcttctcca tcagccccgg ccgctgaagc agaagcatca tggcggaccc
      361 gatggcgttg gtggtggtct cgttgccgcc cgtcaggagc tgatcgagca gatcctggag
      421 ctcgaacatg tccagcggac gctcgggacc accgggctcg tcgacaacct tggcctgcac
      481 gagatcggtg atgatgtcgt cgcgaggctc ggcgcggcgc gcctccatga cctcggcaaa
      541 gaacttctgg gcctcgacca ccagctgccc gcacttcagc gcagccgcct cgtccacgaa
      601 cccaccgcca ggtgcgagca tggcgtcgga ccattccttg aggcgccaga tcttgtcacg
      661 gggaacgccg agctggtcgg cgatcaccgt gcagggcagc gggatggcga attcccagac
      721 cagatccacc tcccccttgt cgatgaactg atcgatgaga tcgttgacca cggtctccac
      781 gtatcccgtc atcttgcgga tccggctgac ggtgaaggcg tggttgatca gcttgcgata
      841 cttcgaatgc accggcggat ccgtccgctg cagcgtggac acgcgccgcc agccatgctc
      901 gcttttgtac tcttcggcga tgttccgctc cgcgccgcgg tggccgccgt gctcgaaaat
      961 gttgttcgaa aagatctcgg ggtgcttctt cacgtggcgg acgtcttcgt agcggctgac
     1021 gtaccacgcg ccgagctctt ccatgaagta gacgggctgc tcgtgcctta tcgcgttgaa
     1081 atagggaaac ggacactctc gcgtctcgtc ctccatcagg ttgtaactcg ccgggtctga
     1141 catgcgctct cccaccaaac acacattcaa ccgctgaatc ctaccagcaa agcgggcggc
     1201 gaacccctag aatctgtaga acagaacagg gaggcaactg ctatgaagat cggagtgttc
     1261 gcaaccttca tgtcgccgct gggcacggcg gagatgatcc aggacttcgg ccgtcgggcc
     1321 gaggcggcgg gtctcgattc catctggatg ggggagcacg tggttctatt cgacgagatg
     1381 gagtttccct acccgggatc ccgggacggt cgcatcccgg ttcccgaggg cggcgggctg
     1441 ctcgacaccg tcgccacctt cgggctgctg gcgggaagca cttcccggct gcgcttcggc
     1501 actggtatct gcctggtccc ccagcgcaac cccatctaca ccgcaaagga gttcgccacc
     1561 ctggactggc tgagcggcgg acggatcgac tttggcatcg gggtgggatg gtgcaaggag
     1621 gaggtaattg cctgcgggta cggctgggaa gaccggggcg agcgatgcga cgagttcctc
     1681 acgctcatgc gcaagctgtg gagcgagccg gtggtcagct actccggcaa gcatgtgaac
     1741 gtgcagggat gtcggatgga ccccaagccg atccagaaac cacacatccc catcatcgtc
     1801 ggcggccaca cccctcgcgc catgcgccgg gccgccgagt tcggcgcggg ctggtatggt
     1861 ttcgggatca ccccggatca gacatctgcc gtgctgaccg agctggacgc ggcgctgggg
     1921 gcagcgggcc ggtcacggga cggcttcgag atcatcatca ccccggcgtc ggaggacgcc
     1981 gacagcatgg ccgcctttgc cgacctgggc gtcgatcgcc tgattctgca cctgggctca
     2041 cagcgcccgg agaagctcgc cgcgcgggta gatcagctgg cgtcactggt ccaagcggcc
     2101 gcctgaaccg gccgtgccgg ccggtggtga gcgctctcgt ggcacaatgg ggccgcgggg
     2161 ggatcctcaa ccagactgga gacatttgct catggcacgc cttgcaggga aaaacatcgt
     2221 cgtcaccggc tcgtcgaggg gcttagggcg cgctttcgcc atcgccatgg ccgcggaagg
     2281 agccagcgtg gtcatcaacg gcaccaatcg ggaggccctg gccgaggtcg agaagaccat
     2341 atcctccgcg ggcggcaacg ttctggcggt ccccggatcc gtcaccgagg agaccacctg
     2401 cgcacagctg gtgtccggct gcgtggaaac cttcgggagc atcgacgtca tgatcaacaa
     2461 cgccggcatc gtgcgcgacc ggacgctcct gaagatgacc gtcgaggagt gggacgacgt
     2521 gctggccgtc aaccttcgcg gcgcgtttgc ctgcaccaag cacgcggcgc tcgccatgcg
     2581 cgaggccggc ggtggccaca tcgtccaggt catctccgcg tcgggcctct ccggcgggtt
     2641 cggtcagggc aactacgccg cggccaaggc gggcatgatg ggcctgctgc gcacggccgt
     2701 gctggagctc tcccgcttca acatccgcaa caacgcgctg tggccggtgg cggagaccga
     2761 catgacccag gtggtgttcg agcgcgcggc cgccatgtcc cgcgagcagg gcgccgagcc
     2821 ccccacaccg gcggagatgg gcttcggcaa gcctgaggaa gtcgcccagg ggctcgtgtg
     2881 gctggtcagc gacggggccg cccatttcga cggccagtgc atgtcgttca acggccgcaa
     2941 gacggccatc tggcgccacc cggcggagga gcacgtgtcg tttcacgacg agccctggag
     3001 cttcgacgag ctcaacgact actaccggga cgtcaaaccg ctacccatct accgaccggt
     3061 gcgcgccgag taggcggatc gctcagcggc cccgggcgcc ccgcgcctgc agcccgaagc
     3121 caatgcaccc gagcaccagg gcgagcagca gacgcggatc gcccaggtgc caccaggggt
     3181 ccagcgggta ggcgatggtc ttctccatgg cggcgatctt gaattcgtgg atgtccaggc
     3241 gactggttcg aaccacctga tccatgagat cccggcggct gcgatagcgc acgagcccgc
     3301 cgttgctcca gcgctccgcg ccgtcgattc cccatagatc caacgcgggg gccgccgcgt
     3361 taccgtagag gatcgggtgc gctgcgctgc ggatggcagc tccaagaaac ggagccgtgt
     3421 accgggccag gacgtcggcg gcggtttcat ccggcgaaac cccctccata gcaagcgggc
     3481 gatcgcgcag atcgacgacg ttgatcatga cgaagtcgtc acccgtgtcc gactccatga
     3541 acgcgcgcca caaggcaagc cgctcgtcgt tcccgcccgc ttcccggagc gcggccaggt
     3601 aggtttcgat ctccgcctcg ctgagcgggc cctggaacga cgtgtaccag tagacgaaca
     3661 ccccatagat tcccagcagg agagcccacg ccttcacttt cgatgcctcg ttttgatcag
     3721 ggtcacccct cccgggccgc ctgctcctcc gcttcggcga ggctgacggc gtaggcgcgc
     3781 cggcccagcc agagcgagag cccggcaatg gccccgaaca ggctggtcag cgtgatcagc
     3841 gagtagcgca cgccctccgc ctcggggaag acgtactcgt tgaatgcccc cgccaccggg
     3901 gggcccagca tcatgcccac ggacaccgtc agcatgtaga gcgccgccac ctgggcacgc
     3961 atgttgggcg gcgtgatcac gggaagagaa ccggcggcga tgccgaacgg ggagttgatg
     4021 aagatcattg ccggcacgta cagcacgaag gcccacatcg gcgagggcac gaactgaatc
     4081 agcagaatcg tcggcaccga aataagcccg ccgatcatgc cggccgtaat gttggcgtcg
     4141 ccccggcccc gctcgcccag cctgcgggcg agcaaggcga tgaggatggg gcccaggggg
     4201 cccgccagaa agaatatcca cccgaacacc tgggacgcat ccgccgcgtc gtaaccgtga
     4261 acgcgaacga agaacgagac cgtccagaac acgaacgcgt agcccatcag cgcgaacgac
     4321 aggaacccga tgtgatgaca cgcgagcgtg cgagcattgc gccggtagaa ggcgacgatc
     4381 tcggcaaagg tcgcttcctg ctgggtcccg gtgcctcccg ccgaacggcg caccggctcc
     4441 ctgagcagca gcaacagcaa agccaggatc atgcccggcg cccccacgta aacgaacgtc
     4501 tgctgccaag ggcgcagctc tccgaccaaa gggagcacca tgggctccgc ctgctcgacg
     4561 atgttcagga ccacgccgcc gatgatgaag gcaatccccg atcccatgat cgccccggtc
     4621 tgaaagatgc tgagcgccag gggaatctga tcccgcggaa agtagtcgcc gaccatcgag
     4681 gtggtggccg gagtgagtgt cgcctcgccg atgcccaccc cgatgcgggc caggaacacg
     4741 ttccagtagc tgcgcgacag cccgcaggca atggtcatca gggaccaaag aaagatgccg
     4801 gccgcgatga tgtgcttgcg gctcttgcgg tcggccagcc aggcgatggg gagcgcggca
     4861 accgcgtaga agagcgcgaa aacgccgtac agccaaccga tctgggcgtc tcccacgccc
     4921 aggtccgcct tcatgggatc caccacgatg gcgaccacct gccggtcgat gaaggcaatg
     4981 agcgcggcga ccaccagaac ggccagcacg aaccacgcgt aggcccggtt cggatacggc
     5041 agagaacgcg cggcggccaa tgcgccgcgt tccccggctc ccgagggcgt gctcagagcg
     5101 cggtgccccc atgcacttcc atgagcgcac cggtgatgcc gccgcccagg tcggaggcca
     5161 gcagaagggc caccgccgcg acttcctcca cggtattgat ccgcttgatg gccgcctcct
     5221 gggcgtagcc gtcgatgtac tgctcgtagg tcatgccgag ggacttggcg gcttcggcac
     5281 cggtgatggt catcaggtcg gtttccacag cgcccggcag gatggcgttg gaggtgatcc
     5341 cgtcgggacc gttctcgtag gccaccacct tggtgagcgc gttgat
//
LOCUS       DBNHKV010000348         5898 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1537938, whole genome shotgun sequence.
ACCESSION   DBNHKV010000348 DBNHKV010000000
VERSION     DBNHKV010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5898)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5898)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5898
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1537938"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="AC16PP_11100"
     CDS             complement(<1..612)
                     /locus_tag="AC16PP_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024924.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6482219.1"
                     /translation="MKEGSLEAPDRQPLDWQNPQFYDKASLYAELERVYDICHGCRRC
                     VSLCDSFPTLFDLVDESETLEVDGVDKADYMKVVDQCFLCDLCAETKCPYLPPHEWAV
                     DFPHLMLRAKAQKFREKDTKWRDRLITSTDPIFDTLSIPGVAQMANAAAGNSALRKLG
                     DKAVGIHPDAPLPNFSSRPLNKRLDTEIGATLTPKPCERTTGKV"
     gene            complement(725..1141)
                     /locus_tag="AC16PP_11105"
     CDS             complement(725..1141)
                     /locus_tag="AC16PP_11105"
                     /inference="COORDINATES: protein motif:HMM:NF014912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HIR6482220.1"
                     /translation="MDLKDSNTLQNLKDAFAGESQANRRYLYFAAKADVEGENDVAAV
                     FRSTAEGETGHAHGHLEYMETVGDPATGLPIGSTGNNLKAAIAGETHEYTDMYPGMAK
                     TARDEGFDEIADWFETLAKAERSHANRFQKALDAMG"
     gene            complement(1338..1937)
                     /locus_tag="AC16PP_11110"
     CDS             complement(1338..1937)
                     /locus_tag="AC16PP_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482221.1"
                     /translation="MAESSRDRVRRHFASVQLTLLSIVVALILENLLSVYWERPQPFE
                     SDALQWLFWLQAGLVLATALSMWSGFALSFNYLHLRQPTTLDIIAPFGLLITMNLAIG
                     AMHPALPAGFLLASGSGSLLAGFILFLDTRNSRRRGKSGPVSALAIQISIALLSIAGA
                     GAIAFEWLGIPGACAVLAVALFTQVTSIVGIARSWRLAT"
     gene            1993..2874
                     /locus_tag="AC16PP_11115"
     CDS             1993..2874
                     /locus_tag="AC16PP_11115"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6482222.1"
                     /translation="MNIELWIIITVAAAFLQNVRSMLQKRLTQRLSVNGATYVRFLYA
                     LPIAWIYLAALASRQDLPLPNSEFLLYCLGGGVGQILATACLVGSFAQRNFAVGTALS
                     KTEVVQAAIFGLLVLGDPVGWRVMMGIAVSLVGVLLLSNRVRLQDIFQADRSVLLGLA
                     SGAAFAVAIVSFRGASLALPSGDYLIRAALTLAVAVSLQSLIMGAYLGRFETGELRRV
                     VRAWRPASLVGLTGMLASAGWLTAVTLESAALVRAVGQVELLFTFAASIWFFGERVGW
                     REAVGVALVMIGIWVLL"
     gene            complement(2871..3440)
                     /gene="idi"
                     /locus_tag="AC16PP_11120"
     CDS             complement(2871..3440)
                     /gene="idi"
                     /locus_tag="AC16PP_11120"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HIR6482223.1"
                     /translation="MSHPATKSEIVSSEEELLILVDGDDREVGFLEKSACHDGAGVLH
                     RAFSLFVLNSAGEVLIQQRASGKRLWPDYWSNSCCSHPRRGETMHEAARRRLHQELGM
                     TAPLEFLYKFEYQADYRGLGAEHELCSVYVGHSDATPVINTTEIGDWRWIARDDLKRE
                     LDTRPEQFTPWFLMEWERILRDHPAALHA"
     gene            3570..4457
                     /locus_tag="AC16PP_11125"
     CDS             3570..4457
                     /locus_tag="AC16PP_11125"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6482224.1"
                     /translation="MSAYGHPREHRFQGSQVELAWYEWGEAPHPKVLLVHATGFHARC
                     WDQVVAHLPPGFHILAVDLRGHGRSTKRGPYVWSSFLADLEEFAAALSLAGAVGVGHS
                     LGGHCITQLAARRPGAFDRLVLVDPVILDPEAYARPRYADLQSAEDHPVARRRDHWAG
                     WEEMFERFSSRKPFSRWRPQVLEDYCRHGLEPRAEGGWRLACPPVVEASIYLGNKGSD
                     VYGMLHDVDVPVAVLRAPGRDPDDHDIMDFSKSPTWPELADRFPRGRDVFLPRLDHFI
                     PMQAPELVARFITDGDADP"
     gene            4454..4708
                     /locus_tag="AC16PP_11130"
     CDS             4454..4708
                     /locus_tag="AC16PP_11130"
                     /inference="COORDINATES: protein motif:HMM:NF016141.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR6482225.1"
                     /translation="MNFLSWLLLGLVVGTLAKLIMPGPDPGGFFVTIGIGMAGAFIGG
                     YGAHLLGIGDVAGFDLASLGLATAGAVLLLGTYRVVRGGF"
     gene            complement(4681..>5898)
                     /locus_tag="AC16PP_11135"
     CDS             complement(4681..>5898)
                     /locus_tag="AC16PP_11135"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6482226.1"
                     /translation="PWYLTSSSLWMAGMSLQGFLFTWLLVGILERPADEAGLARSLAE
                     FPPLAVLLLGGLLGDRFDNRAYLGVMHLLMAVPPLLIAAVYLNGALSYWWVVLFGVLM
                     ASIQALSDPARQAVLSRVSRMDLQRAVTLMMVVTSLVGLSGVYLGGQLDTLGLPTVLA
                     AQAALFAVGLLAVARLPRLPSISARGVRRPSFAEGLRAVWETALIRNIIGLNFISSLF
                     NAGAYIIGIPYIVKEVYAGDAAFLANVMIVFTIGSIGSNVLLLAFMPLKHPGRLYLLL
                     QLTRMFILGLLWLQPSLWLFYVLIVAWGLNMGVTTTLVRTTVQELAPEHHRAQILSVL
                     LLSFVVSSPISSILLGVLIAETEPLTAMLPGIAISLLIFLIGTTRSGLWGYVSPATKT
                     RAAGPQNPPRTTR"
BASE COUNT          969 a         1832 c         1982 g         1115 t
ORIGIN      
        1 gaccttgccc gtggttctct cgcagggttt cggagtcagc gtcgcgccga tttctgtgtc
       61 cagacgcttg ttcagcggtc gcgacgagaa gttcggcagc ggtgcgtccg ggtgaatgcc
      121 gaccgccttg tcgcccagct tgcgcagcgc gctgttgccc gcagcggcgt ttgccatctg
      181 ggccacgccc ggaatgctga gcgtgtcgaa gatggggtcg gtgctggtaa tcagcctgtc
      241 gcgccacttc gtgtccttct cccggaactt ctgcgctttc gcgcggagca tgagatgggg
      301 gaagtccacc gcccactcgt gaggcggcag gtagggacac ttggtctcgg cgcacaggtc
      361 gcacagaaag cactggtcga ccaccttcat gtagtcggcc ttgtcgacgc cgtccacctc
      421 cagggtctcg gactcgtcca ccagatcgaa cagggtgggg aacgagtcgc acaggctcac
      481 gcagcgtcgg cagccgtggc agatgtcgta gacgcgctcg agctcggcgt aaagggaggc
      541 cttgtcgtag aactgcgggt tctgccaatc cagcggttgt cggtctggtg cctcgaggct
      601 gccttccttc atagccatct tcactgcctt tgagttgcgg agactgggtc ggtgtgaata
      661 agtcgttgtg aataaaaagg ggccctatca ggaccccctc cgtttggttg gcggtgaggt
      721 ccgctcagcc catcgcgtcc agcgctttct ggaagcggtt ggcgtgagag cgttcagcct
      781 tggccagggt ctcgaaccag tccgcgatct cgtcgaagcc ctcgtcccgc gcggtcttcg
      841 ccatgccggg atacatgtcg gtgtactcgt gggtctcgcc ggcaatggcc gccttcaggt
      901 tgttgccggt ggagccgatg ggcaggcccg ttgccgggtc gccaacggtc tccatgtact
      961 cgagatgtcc gtgggcgtga ccggtttcac cctctgccgt agagcggaaa acggctgcca
     1021 cgtcgttctc accctccacg tcggccttgg ccgcaaagta gaggtagcgg cggttagcct
     1081 gggattcgcc ggcgaaggcg tccttgagat tctgcagagt gttgctgtcc ttcaggtcca
     1141 tttcttcttg cctcttactc gtttgtcttt ctggtcgaat gaacccgatc gggttcgcgt
     1201 acggtgcagt atacgaactg gaatcccgag atccatttga aagttactat cggcacaata
     1261 gataggaact atcaaaccgg gcttattgct agattctgcg aatgcagatg agaagcgttc
     1321 tcaaaaccgg tgggaggtca ggtggccagg cgccacgatc gggcgatgcc gacgatgctg
     1381 gtgacctggg tgaagagggc gacggcaagc accgcgcacg cgcctggaat tcccagccac
     1441 tcgaaagcga tggcaccggc gccggcgatg ctcagcaagg cgatgctgat ctgtatggcc
     1501 agagcgctga cgggcccgct tttgccgcgt cggcgcgagt tgcgcgtgtc gagaaagaga
     1561 atgaagcccg ccagaagaga cccggagcct gacgccagca ggaaccctgc cggcagcgcg
     1621 gggtgcatgg ccccgatcgc caggttcatc gtgatcagca ggccgaaggg cgcaatgatg
     1681 tcgagcgtgg tgggctgccg gaggtgcaga tagttgaagc tcagggcgaa accggaccac
     1741 atcgacagcg cggtggccag aaccagcccg gcctggagcc agaacagcca ctgcagggca
     1801 tcgctttcga agggctgcgg gcgttcccag taaacgctca gcaggttctc caggatcagg
     1861 gcgactacga tggacagcag ggtgagctgc acgctggcga agtgacgtcg cacgcggtct
     1921 cgactggatt ccgccatttg cagagtctcc gggtggttgc aatgactacg ctaccttaag
     1981 aaggtagcgg tcgtgaacat cgaactctgg atcatcatca cggtcgcggc cgcgtttctg
     2041 cagaacgttc ggtccatgct ccagaagcgt ctgacccaac gcctttcggt gaacggcgcg
     2101 acctatgtcc gcttcctgta cgcgctgccc atcgcctgga tatacctggc cgccctggcg
     2161 tcacgccagg acctgccgct ccccaactcg gaattcctgc tttactgcct tggcggcggc
     2221 gtgggccaga ttctggccac cgcctgtctc gtcggctctt tcgcgcagcg caactttgcc
     2281 gtcggcaccg cgctctccaa gaccgaggtg gtgcaggccg ccatcttcgg gctgctcgtg
     2341 ctgggcgatc ccgtcggctg gcgggtgatg atgggcattg ccgtgagcct ggttggtgtg
     2401 ctgcttcttt ccaaccgcgt gcgcctccag gacatttttc aggctgatcg ctccgtgctc
     2461 ctggggctcg ccagcggggc cgcgtttgcg gttgccatcg tcagctttcg cggagcgtcg
     2521 ctcgcgctgc cctccggaga ctacctgatc agggcggccc ttaccctggc ggtggccgtc
     2581 tctctgcagt cgctcatcat gggcgcgtac ctcggccggt tcgagacggg agagctgcga
     2641 cgcgtcgtcc gggcctggcg ccctgcgtct ctcgtggggc tcacggggat gctcgcgtcg
     2701 gccggatggc tcacggcggt gactttagag agcgcggcgt tggtgcgggc cgtggggcag
     2761 gtagagctgc ttttcacgtt cgcggcctcc atctggttct tcggcgaacg ggtcggctgg
     2821 cgcgaagcgg tgggcgtcgc cctggtcatg atcggtatct gggtgttgct ctaggcgtgc
     2881 agggccgcag ggtggtcgcg caggattcgt tcccactcca tgaggaacca gggcgtgaac
     2941 tgctccggcc gcgtgtcgag ctcccgtttc aggtcgtccc gggcgatcca gcgccagtcg
     3001 ccgatctcgg tggtgttgat gacgggtgtt gcgtcgctgt ggccgacgta gacggagcag
     3061 agctcgtgct cggcgcccag cccgcggtag tccgcctggt attcgaactt gtagaggaac
     3121 tcgagcggcg cggtcatgcc gagctcctga tgcagcctgc ggcgcgcggc ctcgtgcatg
     3181 gtctctccgc gccgtggatg gctgcagcag ctgttggacc agtagtcggg ccacagccgc
     3241 ttgccggagg cccgctgctg gatgagcacc tctccagcgg agttgagcac gaacagggag
     3301 aacgccctgt gcagcacgcc ggccccgtcg tggcaggcgg acttttccag aaaaccgacc
     3361 tcacgatcat cgccgtccac gaggatcagc agctcctcct ctgaggagac gatctcggat
     3421 tttgttgctg ggtgagacat gaagaccggc tcggcgtgag aaagatcagt gtagcggaat
     3481 tggcgacggc ggtctgcgtg tcttgtctgg cctgcccgct ctccgtagca tgatcacgtt
     3541 gcggccatcg gccgcgcgat cagtttttca tgagcgcata cggacatccc agggagcacc
     3601 gttttcaggg ctctcaggta gagcttgcct ggtacgaatg gggcgaggcg ccgcatccca
     3661 aggtgctgct cgtgcatgcc accggcttcc acgcccgctg ctgggatcag gtggtcgctc
     3721 atctcccccc gggttttcac atcctcgccg tggacctgcg cggccatggc cgcagcacca
     3781 aacggggtcc ttacgtgtgg agcagtttcc tggcagacct ggaagagttt gccgccgcgc
     3841 tgagcctggc gggtgccgtc ggcgtcgggc actctctggg cggtcactgc atcacgcagc
     3901 tggcggcgcg gcgtccgggc gcattcgacc ggctggttct ggtcgacccc gtcatcctgg
     3961 atccggaagc ttacgcgcgc ccgcgatatg ccgacctgca gtcggcggag gatcaccccg
     4021 ttgcccggcg tcgggatcac tgggccggct gggaagagat gttcgaaagg ttctcttcgc
     4081 gcaagccgtt ctcgcgatgg aggccgcagg tgctggagga ctactgccgc cacggcctcg
     4141 agccccgtgc ggaaggcggc tggcgtctgg cctgcccgcc cgtggtggaa gcttcgattt
     4201 acctcggaaa caagggcagc gacgtctacg gcatgctgca cgatgtcgac gtgccggtag
     4261 ccgtgctgcg agcgcccggg cgcgaccccg atgaccacga catcatggat ttctccaagt
     4321 cgccgacctg gcccgagctg gcggacaggt ttccgcgggg gcgggacgtc tttctgccgc
     4381 ggctcgatca cttcattccc atgcaggcgc cggaactggt ggcccgcttc atcaccgacg
     4441 gagatgcaga cccgtgaact tcctatcctg gctccttctc gggctcgtcg ttggcaccct
     4501 cgccaagctg atcatgcctg gccccgatcc gggcggcttt ttcgtcacca tcggcatcgg
     4561 gatggcgggc gcgttcatcg ggggttacgg cgcccacctg ctggggatcg gcgacgtggc
     4621 cggattcgat ctggctagcc tcggccttgc caccgccggc gcggtgctgc tcctggggac
     4681 ctaccgggtc gttcgcggcg ggttctgagg ccctgcggct cgagtcttgg tcgcggggga
     4741 aacgtagccc cacagcccgc tgcgcgtggt cccgatcagg aagatgagca gagagatggc
     4801 gatgccggga agcatcgcgg tcagcggctc ggtctcggcg atcaacacgc ccagcaggat
     4861 cgagctgatg ggcgaggaca ccacgaagct caagagcagc acggagagga tctgcgctct
     4921 gtgatgttcc ggcgccagct cctgcaccgt ggttctgacc agggtggtgg tgacgcccat
     4981 gttcaggccc caggccacga tcagcacgta gaacagccac aggctcggtt gcagccataa
     5041 aaggcccagg atgaacatcc gggttagctg cagtagcagg tagagccttc ccggatgctt
     5101 cagcggcatg aaggccagca ggagcacgtt ggagccgatg ctgccgatgg tgaacacgat
     5161 catcacgttc gccaggaacg cggcgtcccc cgcatagacc tccttgacga tgtaggggat
     5221 accgatgatg tacgcgccgg cattgaacag gctggagatg aagttcagac cgatgatgtt
     5281 gcggatcagc gcggtctccc acacggcccg caggccttcc gcgaagctcg gccggcgcac
     5341 cccgcgcgcg ctgatgctgg gcaggcgcgg caggcgtgcc accgcgagga gaccgaccgc
     5401 gaacagcgcc gcctgggcgg ccaggacggt gggcagaccg agcgtgtcga gctggccgcc
     5461 gagatagacg ccgctcaacc cgaccagtga ggtgaccacc atcatcagcg tcaccgcccg
     5521 ctgcaggtcc atgcgggaca cgcggctgag cacggcctgc ctggccggat cgctcagcgc
     5581 ctggatgctg gccatgagca cgccgaacag aaccacccac caatagctga gcgcgccgtt
     5641 gaggtaaacg gcggcgatca gcagcggggg aacggccatg agcaggtgca tgactcccag
     5701 ataggcccga ttgtcgaacc ggtcgcccag caggccgccg agcagcagga cggccagcgg
     5761 tgggaactcg gccagcgatc gggcgaggcc cgcctcatcc gccggccgct ccaggatgcc
     5821 cactaaaagc caggtgaaaa ggaagccctg aaggctcatg cccgccatcc acaggctgga
     5881 gctggtcagg taccaggg
//
LOCUS       DBNHKV010000349         9217 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_47721, whole genome shotgun sequence.
ACCESSION   DBNHKV010000349 DBNHKV010000000
VERSION     DBNHKV010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9217)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9217)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9217
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_47721"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="AC16PP_11140"
     CDS             complement(<1..311)
                     /locus_tag="AC16PP_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784357.1"
                     /note="Sms; stabilizes the strand-invasion intermediate
                     during the DNA repair; involved in recombination of donor
                     DNA and plays an important role in DNA damage repair after
                     exposure to mutagenic agents; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR6482227.1"
                     /translation="MAGKRKAKSAYVCSDCGAEHTQWQGQCVACSAWNTLTRVSIGPI
                     KEPVSRGYAGSQSQVSLLGEVEREEAERLPTGFAELDRVLGGGLVPGSVVLMGGDPGA
                     G"
     gene            complement(311..1744)
                     /gene="dnaB"
                     /locus_tag="AC16PP_11145"
     CDS             complement(311..1744)
                     /gene="dnaB"
                     /locus_tag="AC16PP_11145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR6482228.1"
                     /translation="MPDSATTPAEAHENETQRPAVAAVSHTAFKTPPHSVEAEQSVLG
                     ALMLNDEAWFNVAEIVTARDFYRPQHQIIFEAMAELSADTQPLDAVTVSERLQSKGLL
                     DKAGGNAYLGELAESIPGTSNVVAYAQIVREHSTLRQLISAANRIAETAFAPDGRPSG
                     ELLNMAEQEVFRIAEGRLRDSGPEKVVPLLHRAVERIEKLYETRNPITGVATGFDDLD
                     KKTAGLQPSDLVIIAARPSMGKTALAVNIVEHAVMEGSGAVLMFSLEMPSEQIVMRML
                     SSLGRIDQTRMRTGDMHEDDWPRFTSAVSQLKDKPLYLDDTPALTPNDIRTRARRVAR
                     ESGGLGLVVVDYLQLMRGSGPVENRTNEISEISRSLKAIAKELRCPVVALSQLNRGLE
                     SRTDKRPVMADLRESGAIEQDADVILFIYRDEVYNPESEQKGTAEIIIGKQRNGPIGM
                     CRLSYIGNLTKFENLAPDRYNDFQPFD"
     gene            complement(1830..2276)
                     /gene="rplI"
                     /locus_tag="AC16PP_11150"
     CDS             complement(1830..2276)
                     /gene="rplI"
                     /locus_tag="AC16PP_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR6482229.1"
                     /translation="MNIILLEKIGSLGDLGEEVTVKGGFARNYLIPQGKAVRATTENR
                     GVFEERRAELERVAEEQRAAAQSRAEGLEGKVVTIVAKAGEEGKLYGSVGTQDIVDAL
                     HNEQIEIARSEVRMPEGVIRVTGEYEIDVQLHSDVTVTIQVNVVAE"
     gene            complement(2287..2511)
                     /gene="rpsR"
                     /locus_tag="AC16PP_11155"
     CDS             complement(2287..2511)
                     /gene="rpsR"
                     /locus_tag="AC16PP_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR6482230.1"
                     /translation="MSRRFGRKKFSRLTGDDVNIDYKDLDLLKQYIGETGKIVPSRIT
                     GTSARHQRQLAQEIKRARFLALLPYTDQHK"
     gene            complement(2508..2996)
                     /gene="rpsF"
                     /locus_tag="AC16PP_11160"
     CDS             complement(2508..2996)
                     /gene="rpsF"
                     /locus_tag="AC16PP_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR6482231.1"
                     /translation="MRHYEVVFLVHPDQSDQVPGMVERYQSLIERGTGVVHRLEDWGR
                     RQLAFPISKIHKAHYLLMNIEVNRETLEELESAFRFNDAVLRYLTIRRKAAVTGNSKI
                     YEEELRDQEKERERDQRREEETAQRRREREEAASAASDEAPAEEPAGESAATEAGEEV
                     TE"
     gene            complement(3118..3864)
                     /gene="rlmB"
                     /locus_tag="AC16PP_11165"
     CDS             complement(3118..3864)
                     /gene="rlmB"
                     /locus_tag="AC16PP_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR6482232.1"
                     /translation="MTDGSKVHGIHAVTAVLRDDPARVRVVYCITGRVDARMREVLEE
                     ARAAGVRVERVDRRQLDRLAGSGHQGVAAHCHELALADERAFAEHLDTLPEPWLMLAL
                     DGVTDPRNLGACLRSAEAAGVQAVLLPRRRSAPLNAAALKAAAGAAEGLYLVEVANLA
                     RRLEWLRDRGVWVVGAAGEADTPWTEADLDGNCLIVVGSEGQGLRQLTARSCDQLVGI
                     PMAGTVSSLNVSVAAGVLLFEAVRQRRARG"
     gene            complement(3861..6062)
                     /gene="rnr"
                     /locus_tag="AC16PP_11170"
     CDS             complement(3861..6062)
                     /gene="rnr"
                     /locus_tag="AC16PP_11170"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIR6482233.1"
                     /translation="MATRTKKTNSSSKITARDVRESLGRGRGPLAWRELVAATVGDSA
                     SGRRELRRVLRGLVSAGEVVQIPDGRYQLEEPAGVERGVVALHRGRLCVGEIPLETGR
                     RVRLRPGDVVEMQIGQGADGDIARVLRVIEYSAEPIIGQLNGRGRHPYVDSLSPHYKG
                     RVSLVDPPAFGNHGDTVSVRIVDEDRRGLVGYVEALVSDRRGAAHAAETMLASHGVPR
                     QWPSAVLSAAQRLPKAVVPARHPDRRDLAGLPLVTIDGETARDFDDAVYAARRRGGWR
                     LVVAIADVAHYVKPKGALDAEALERGNSVYLPDRVVPMLPEAISNGLCSLKPREPRLA
                     MVCDMQVSNAGNVTRFEFYEAVIRSWQRLTYKRVQEFLDSGALDVEPEVCRSLADLQG
                     TFSALRVARDQRGALDFDTHEATLELSGDHVAAIHPFIRTDAHRLIEEAMIAANVCAA
                     TFVEKHGVSALYRVHEGPSGERDQRVRQAFAYAGIRLPRGRLDPAMLRQALDGLGERD
                     DRWVFEMLVLRSLTQAVYRPENKGHFGLALTRYMHFTSPIRRYADLVVHRAIKAALHG
                     APPPAADDWLVACGEQVSMTERRADEVSWAVDGWLKCEYVSSRIGEVFPGVVMGVTDF
                     GLFVELTGYYVQGLLHVSDLGSDYFQYQPATLALVGERSNRRFGLGDHLTVRLAGVQP
                     ALGRLDLELVEPAAGSASPRTNRRRPAADRETGRGGARGGARGGSRGRKRR"
     gene            6109..6195
                     /locus_tag="AC16PP_11175"
     tRNA            6109..6195
                     /locus_tag="AC16PP_11175"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6143..6145,aa:Leu,seq:cag)
     gene            complement(6258..7391)
                     /locus_tag="AC16PP_11180"
     CDS             complement(6258..7391)
                     /locus_tag="AC16PP_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011332951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR6482234.1"
                     /translation="MSKPVVISGAGLWKPDHVITNAELVAAYNAYADRFNAENDAAIG
                     AGDVAPMEHSSEEFIEKASGIKQRYAYVKDGILEVGRMRPRIPHREEDELSHQAEIAL
                     HAARGAMAAAGKAASDIDMVIVSCAYTERSYPAIAIEVQHALGIDGFAFDMLVACSAA
                     TFALQRAHDAIAAGSARCVLVVNPELTSPQVNYRDRDGHFIFGDVSTAVVLESAETCT
                     AAHAYEILGSKALTSYSNNIRSNFGYLAYAEDVDPFCAANLFHQEGRRVFKEVCPMAA
                     EHLKSQVEAVGLDVADVKRWWLHQANLNMNLLISKRLLGRDASAKEAPIVLDEYANTA
                     SAGSIIAFCLHHEDLAAGDHGVICSFGAGYSIGSLVLRKRAAA"
     gene            complement(7392..8342)
                     /gene="folE2"
                     /locus_tag="AC16PP_11185"
     CDS             complement(7392..8342)
                     /gene="folE2"
                     /locus_tag="AC16PP_11185"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HIR6482235.1"
                     /translation="MNKGELRSEKELPDVASHELHQPHVTLDWVGMADIHQPLLVRDG
                     NQTRHVQSRVQIYVDLGDELAKGIHMSRLYLILDEHAETRPLSAAGLKLLLRSVLESH
                     RELSSRAYVQFDFDYYLRRQALVSDNSGWNSYPVSIRGTLIDGEITLEVALNVQYSST
                     CPCSAALARQLIQDQFDEDFGTSGSVAVGDVRAWLGTEQGIVATPHSQRSDARIRVKL
                     REGLIDFPITDLIDEVEATLKTPVQTAVKREDEQEFARLNGQNLMFIEDAGRKLKDQL
                     DADDRLADFWVRVEHHESLHAHDAVGVFTKGVGDGYQAVP"
     gene            complement(8478..8669)
                     /locus_tag="AC16PP_11190"
     CDS             complement(8478..8669)
                     /locus_tag="AC16PP_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008375064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIR6482236.1"
                     /translation="MDSKLLELLVCPVTKASLTYNKDKDELWCRASGLAYPIRDGIPV
                     MLEEEARELSAEERESLRE"
     gene            complement(8697..>9217)
                     /locus_tag="AC16PP_11195"
     CDS             complement(8697..>9217)
                     /locus_tag="AC16PP_11195"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HIR6482237.1"
                     /translation="LDYILGITKAYSTRVGSGPFPTELFDEVGERLATRGNEFGSTTG
                     RPRRCGWFDAVALRQAVQINSISGLCLTKLDVLDGLESICICVGYEGPDGTSGTPRFA
                     SEYYSEIRPVYEEVDGWSESTLGIRRLDDLPANARAYLARIEETVGAPIDIISTGPDR
                     TETIVLKDPFTA"
BASE COUNT         1543 a         3123 c         2905 g         1646 t
ORIGIN      
        1 tttcccgcgc cgggatctcc acccatcagc acgacggagc cgggcaccag gccgccgccg
       61 agcacccggt cgagctcggc aaatccggtg ggcaaacgct cggcctcctc gcgctccacc
      121 tcgccgagca ggctaacctg gctctggctg ccggcgtagc cccgggaaac gggttctttg
      181 atgggcccga tggaaacccg ggtgagggtg ttccaggcgc tgcaggccac gcactggccc
      241 tgccactggg tatgctcggc gccgcagtcc gagcagacgt acgcggattt ggctttgcgc
      301 ttgcccgcca tcagtcaaat ggctgaaagt cgttgtagcg atccggcgcc aggttttcga
      361 acttggtcag gttgccgata taggacagac ggcacatccc gatggggccg ttgcgctgct
      421 tgccgatgat gatctcggcg gtgcccttct gctcggattc ggggttgtaa acctcgtcgc
      481 ggtagatgaa aaggatcacg tccgcgtcct gctcgatggc gcccgactct ctaagatcgg
      541 ccatgaccgg ccgcttgtcg gtacggctct ccagcccccg attgagctgg gaaagcgcga
      601 ccaccgggca gcgcagctcc ttggcgatgg ccttcagaga tcgggagatc tccgaaatct
      661 cgttcgtgcg gttctccaca ggtccggaac cgcgcatgag ctgcaggtag tcgaccacca
      721 ccaggccgag gccgccggat tcgcgggcta cccgccgcgc ccgggtgcgg atgtcgttcg
      781 gcgtcaacgc aggcgtatcg tccaggtaca gaggtttgtc tttcagctgg ctgacggcgg
      841 aggtgaaccg cggccagtcg tcttcgtgca tgtccccggt gcgcatgcgg gtctgatcaa
      901 tcctgcccag cgatgagagc atgcgcatga cgatctgctc ggacggcatc tccaggctga
      961 acatgagcac ggcgccgctg ccttccatca ccgcgtgctc gacgatgttg accgcaaggg
     1021 ccgttttgcc catggacggc cgcgcggcga tgatcaccag atcggacggc tgcaggccgg
     1081 ccgtcttctt gtccagatcg tcgaagccgg tggccacgcc ggtaatgggg ttgcgggtct
     1141 cgtagagctt ctcgatgcgc tccaccgccc ggtgcagcag cgggacgacc ttttcgggtc
     1201 cactgtcgcg cagacgccct tcggcgatgc ggaagacctc ctgctcggcc atgttcagca
     1261 gctccccgct gggcctgccg tcgggagcga acgccgtttc cgcaatgcgg ttggccgcgc
     1321 tgatcagctg acgcaacgtg gagtgctcgc gcacgatctg cgcgtaggcc accacgttgc
     1381 tcgtgccggg aatcgactcg gccagctcgc ccaggtaagc gttgccgccc gccttgtcga
     1441 gcaggccctt ggactgcagc cgctccgaga cggtcaccgc gtccagaggc tgcgtgtctg
     1501 ccgacagctc cgccatggct tcgaagatga tctgatgctg cggccggtag aagtcccggg
     1561 cggtgacgat ctcggcaacg ttgaaccacg cttcgtcgtt cagcatcagc gcgcccagca
     1621 cggactgctc ggcctctacg gagtggggcg gcgtcttgaa cgccgtgtgg ctcacggccg
     1681 cgacggctgg tcgctgagtt tcgttttcgt gagcctctgc gggcgtggtt gcgctgtcag
     1741 gcatcgctgg acgactttcc gcgggggagg aaaagactct gtctttccgg tccccattgc
     1801 aagcgaagat tgcctatcgg cgcgagaaat tactcggcga cgacgttcac ctgaatcgtg
     1861 acggtcacgt cggagtgcag ctgcacgtcg atttcgtact cgccggttac ccggatcacg
     1921 ccttcgggca tgcgaacctc gctgcgagca atctcgatct gctcgttgtg cagggcatcc
     1981 acgatgtcct gggttcccac ggatccgtac agcttgccct cctcgccggc cttcgccacg
     2041 atggtgacga ccttgccttc gagtccctcg gcgcggctct gggcagctgc acgctgctct
     2101 tcggccacac gctcgagctc cgccctgcgc tcctcgaaca ctccccgatt ctcggtggtc
     2161 gcccgaacgg cctttccctg agggattaga tagttcctgg caaacccacc tttgacggtc
     2221 acttcctcgc cgagatcgcc gaggctgccg atcttttcca gcagaatgat gttcatgatc
     2281 gaagacctac ttgtgctgat cggtgtatgg cagaagcgct aagaacctgg cgcgcttgat
     2341 ctcctgggcc agctgacgct gatggcgcgc gctggtgccc gtgatccggc tggggacgat
     2401 cttccccgtt tcgccgatat actgcttgag cagatccaga tccttgtagt cgatgttgac
     2461 atcgtcgccg gtcagccggc tgaacttctt gcgtccgaat cgtctgctca ttccgtcacc
     2521 tcctctccgg cttcagttgc ggctgattcg ccggcgggct cctccgcggg cgcttcatcg
     2581 gaagccgcgg aagcggcctc ctccctttca cggcgccgct gagccgtttc ttcctcacga
     2641 cgctggtcgc gctcgcgctc tttctcctgg tcgcgcagct cctcctcgta gatcttggag
     2701 ttgccggtga ccgccgcctt gcggcggatg gtcagatacc gcagaaccgc gtcgttgaaa
     2761 cggaacgcgc tctcgagctc ctccagggtc tcacggttga cctcgatgtt catcagcagg
     2821 taatgggcct tgtgaatctt cgagatggga aaggccagct ggcggcggcc ccagtcctcc
     2881 agacgatgga cgacgccggt gccacgctcg atgaggctct ggtagcgttc gaccattccg
     2941 ggcacctgat cgctctgatc ggggtgcacg agaaaaacga cttcgtaatg tcgcatgttg
     3001 cgaattctcc ttttggcgtt accgcctccg ctcgcagagg tttgcggcgt gaggcaagga
     3061 cccctccgac gaggggacgc gaattgtagg taggcgccag atcggatgca agggcgctca
     3121 gcccctggcg cggcgctgcc gaacggcctc gaaaaggagc acgccggcgg ccacggagac
     3181 gttcaggctc gacacggtgc ccgccatggg gatgcccacc agctgatcac agctgcgcgc
     3241 ggtgagctgt cgcagaccct gcccctcact gccgacgacg atgagacagt tgccgtcgag
     3301 atccgcctcc gtccagggcg tatcggcctc gccggccgcg cccaccaccc agacgccgcg
     3361 gtcccgaagc cactcgagcc ggcgggccag attggcgacc tccaccagat agaggccttc
     3421 cgctgcgcca gccgccgctt tcagagccgc cgcgttcagg ggcgcgctgc gccgcctggg
     3481 aagcagaacc gcctgcacgc ccgccgcctc agccgagcgc aggcaggcgc cgagattgcg
     3541 cggatccgtg accccgtcga gcgccagcat gagccaaggc tcgggaaggg tgtcgagatg
     3601 ctcggcaaac gcacgctcgt cggccagggc cagctcgtgg cagtgcgcgg cgacgccctg
     3661 gtggcccgag cctgccaggc gatcgagctg acgtcggtcg acccgctcga cccgtacccc
     3721 ggctgcgcgg gcttcctcca gcacctcgcg catccgggca tcgacgcgac cggtgatgca
     3781 gtaaaccacc cggacccgcg ccgggtcgtc gcgcagcacg gcggtcacgg catggatccc
     3841 gtgtaccttg gaaccatccg tcatcgacgc ttcctgcctc gggaaccgcc gcgggcgccg
     3901 ccgcgggcgc cgccgcggcc agtctcacgg tcagcggcgg ggcggcgtcg gttcgtccgc
     3961 ggactggcag agccggcagc gggctcgaca agctcgagat cgagccggcc cagggccggc
     4021 tgcacgcccg cgagccggac ggtgaggtgg tcgcccagac cgaaacgccg gttcgagcgc
     4081 tcgcccacca gcgccagcgt cgccggctga tactgaaagt agtcgcttcc cagatccgac
     4141 acgtgcagca atccctgcac gtagtagccg gtcagctcca cgaaaagacc gaaatcggtc
     4201 acacccatga ccacgccggg gaacacctcg ccgatacgcg aggagacgta ctcgcatttc
     4261 agccatccgt ccacggccca gctgacctcg tccgcacgcc gttccgtcat ggagacctgc
     4321 tcgccgcagg ccaccagcca atcgtccgct gccgggggcg gggcgccatg gagcgcggcc
     4381 ttgatcgccc tgtgaaccac caggtccgca tagcgtcgga tgggagaggt gaaatgcatg
     4441 taccgcgtca gcgcgaggcc gaagtgcccc ttgttctcgg ggcggtagac ggcctgggtc
     4501 aacgaccgca gcacgagcat ctcgaacacc cagcggtcgt cccgttcccc caggccatcc
     4561 agggcctgac gcagcatcgc cggatcaagc cgcccccgcg gcaggcgaat cccggcgtag
     4621 gcgaacgcct gccgtacccg ctggtccctt tctcccgacg gcccttcgtg cacccgatac
     4681 agagcggaga ctccgtgctt ttcgacaaac gtcgccgcgc acacgttggc ggcgatcatg
     4741 gcttcctcga tgagccggtg ggcgtccgtg cggatgaagg ggtgaatggc cgcaacgtga
     4801 tctccggaaa gctccagcgt cgcctcgtga gtgtcgaaat ccagcgcccc gcgttgatct
     4861 cgggccaccc gcagggcgct gaacgtcccc tgcaggtcgg caagcgaccg gcagacctcg
     4921 ggctccacgt ccagcgcgcc ggaatccagg aattcctgta cccgcttgta ggtgagccgc
     4981 tgccaggagc ggataaccgc ttcgtagaac tcgaaacgcg tgacgttgcc cgcgttcgaa
     5041 acctgcatgt cgcaaaccat ggcaagccgg ggctctcgag gtttcagcga gcagagcccg
     5101 ttggagatcg cttccggcag catcggcacc acccggtcgg gcaggtaaac cgagttgccc
     5161 cgctccagcg cctccgcgtc cagcgcacct ttcggcttca cgtagtgagc aacatcggcg
     5221 atcgcgacga ccagacgcca cccgccgcga cgccgggcgg cgtataccgc atcgtcgaaa
     5281 tcccgcgcgg tctcaccatc gatggtcacc agcggcagac cggccaggtc gcgccgatcc
     5341 ggatgacggg ccggaaccac cgccttgggc agcctctggg cggcactgag caccgccgac
     5401 ggccactgtc tgggcacacc gtggctggca agcatggttt ccgcggcgtg ggcggcgccg
     5461 cggcgatccg aaacgagcgc ctcgacatat cccaccagtc cacgtcgatc ctcatcgacg
     5521 atgcgcaccg agacggtgtc gccgtggttg ccaaaagcag gcggatcgac cagggagacg
     5581 cgccccttgt agtgcgggct cagggagtcg acatagggat gtcgcccgcg cccgttgagc
     5641 tggccgatga tcggttccgc cgagtactcg ataacgcgca ggacacgggc gatgtcgccg
     5701 tccgccccct gaccgatctg catctcgacg acgtcgcccg gtctgaggcg aacccggcgg
     5761 cccgtctcca agggaatctc gcccacgcac agccggccgc ggtgcagagc cacgacaccc
     5821 ctttccacgc cagcgggctc ctcgagctgg tagcgcccat cgggtatctg caccacctcg
     5881 ccggcgctga cgagcccacg cagcacgcgg cgaagctccc ggcggccgga tgccgagtcg
     5941 ccgacggtgg ccgccaccag ctcccgccag gcgagcggac cccggccgcg gcccagggat
     6001 tcccggacat cgcgggcggt gatcttggag gaactgttcg tctttttcgt tcgcgtcgcc
     6061 atcgtttgac aaggagcggg gcacttcagt aaattgcgcc cccctcgtgc cgaggtggtg
     6121 aaattggtag acacgctagc ttcaggtgct agtgggggca accccgtgga ggttcaagtc
     6181 ctctcctcgg caccacattt tagagcgtgc ccggcgaccc cgaaacagga attctctttg
     6241 agcggcgcgc cctcacatca cgccgccgcc cgctttctca acaccaggct gccgatcgag
     6301 taacccgcac caaacgagca gatcacgcca tggtctccgg cggcgaggtc ctcgtgatgc
     6361 agacagaacg cgatgatcga gcccgccgac gccgtgttgg catactcgtc cagcacgatg
     6421 ggcgcttcct tcgccgaggc atcgcggcca agcagccgct tgctgatcag caggttcatg
     6481 ttcagattgg cctgatgcaa ccaccagcgt ttcacatcgg ccacgtccag tcccaccgcc
     6541 tccacctggg atttcaggtg ctcggccgcc atggggcaga cctccttgaa cacacgacgg
     6601 ccttcctggt gaaagaggtt ggcggcgcag aaggggtcca cgtcctcggc atatgccagg
     6661 tagccgaaat tggaccggat gttgttcgag tagctggtca gcgctttgct gccgagaatt
     6721 tcgtacgcgt gcgcggcggt gcaggtttct gcggactcga gcacaaccgc cgtgctcacg
     6781 tcaccgaaga tgaagtggcc gtccctgtcg cgatagttca cctgaggcga ggtgagttcc
     6841 gggttcacca ccaatacgca tcgcgcggac ccggccgcta tggcgtcatg agcacgctgc
     6901 agcgcaaacg tcgccgctga gcacgcgacg agcatatcga aggcaaagcc gtcaatcccc
     6961 agggcgtgct gaacctcgat ggcgatagca gggtaggagc gctcagtgta ggcgcaggag
     7021 acgatgacca tgtcgatgtc cgacgccgcc ttacctgccg cagccatggc gccccgggcg
     7081 gcgtggagag cgatttcagc ctggtgggaa agctcgtcct cctcgcggtg aggaatgcgc
     7141 ggacgcattc gccccacctc caggatcccg tccttcacgt aggcgtaacg ctgcttgata
     7201 ccagaggcct tctcgatgaa ctcctcgctc gagtgctcca tcggcgcgac gtcgccagcc
     7261 ccgatggcag cgtcgttctc cgcattgaat ctgtccgcgt acgcgttgta ggcggcgacg
     7321 agttctgcat tggtgatgac gtggtcgggc ttccacagcc cggcgccact gatgacgacg
     7381 ggtttcgaca cctagggaac tgcctggtat ccgtccccga cacccttcgt gaacaccccc
     7441 acggcgtcgt gggcatggag gctctcgtgg tgctccacgc gcacccagaa gtccgcgaga
     7501 cggtcgtccg catccagctg atccttgagc ttgcgtccgg catcctcgat gaacatgaga
     7561 ttctggccgt tgagcctggc gaactcctgc tcgtcctccc gcttgacggc ggtctgcacc
     7621 ggcgtcttca gcgtggcttc cacctcgtcg atcagatcgg ttatgggaaa gtcgatgagg
     7681 ccttcgcgca gcttgacgcg aattcgcgcg tcgctgcgct ggctgtgcgg cgtcgccacg
     7741 atgccctgct cggtgcccag ccaggcacga acatcgccca cggccacgct cccggaggtg
     7801 ccgaagtcct cgtcgaactg atcctgaatc agctgacgcg ccagggccgc ggagcacggg
     7861 cacgtcgacg agtactgcac gttgagggcg acctccaggg taatctctcc gtcgatcagc
     7921 gtgcccctga tggacaccgg atacgaattc cagccggagt tgtcgctcac cagagcctgc
     7981 cggcgcaggt agtaatcgaa atcgaactgc acgtatgccc gggagctcag ctcgcgatgg
     8041 gactcgagca cgctgcgcag cagcagcttc aagcccgccg cgctcaaggg ccgggtctcg
     8101 gcatgctcgt cgaggatcag gtagaggcgc gacatgtgaa tgcccttggc cagctcgtca
     8161 cccaggtcca cgtagatctg aacacgggac tggacgtgcc tggtctggtt tccatcacgc
     8221 acgagaagag gctgatggat gtcggccatg ccgacccagt ccagggtcac gtgcggctga
     8281 tgcagctcgt gactggccac atcgggcagt tccttttcgc tgcgaagctc gcccttgttc
     8341 atgggtctac ctgcatgtgt tcttcctcca gctacccccc ccgtgaaacg ggcggccccg
     8401 gattgtaccc aatcggcctg caacggcaca gaaggtgccg ccggccggcg cggagcccgg
     8461 ttccgggggc cggagcatca ctccctcagg ctttcccgct cttcggcgct gagctcgcgg
     8521 gcttcctcct cgagcatcac ggggatgccg tctcggatgg ggtaggcgag gccgctggcg
     8581 cggcaccata gctcgtcctt gtctttattg taggtcagcg acgccttggt gacggggcag
     8641 accagcagtt cgagcagctt cgagtcaatc gacatgacgt tgcgcctcca ccgatctcag
     8701 gcagtgaacg gatccttcag aacaatggtt tcggtccggt ccggacccgt cgagatgatg
     8761 tcgatgggag cacccaccgt ctcctcgatg cgggccagat acgcccgcgc attggcaggc
     8821 aggtcatcca ggcgccggat gcccagggtg gactccgacc agccgtccac ctcttcgtac
     8881 acgggcctga tttccgagta atactcgctg gcaaaccgcg gcgtgccgct cgttccgtcc
     8941 gggccctcgt aacccacgca gatgcagata ctctccagac cgtcgagcac gtccagcttg
     9001 gtcaggcaga gcccggagat gctgttgatc tgcacggcct gccgcagggc cacggcatcg
     9061 aaccagccgc accggcgcgg cctgcccgtg gtggacccga actcgttgcc gcgggtcgca
     9121 agccgctcgc caacctcatc gaagagttcc gtcggaaacg gtcccgagcc cacgcgggtg
     9181 gaatatgcct tcgtgatgcc caggatgtaa tccagat
//
LOCUS       DBNHKV010000350         6214 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_687199, whole genome shotgun sequence.
ACCESSION   DBNHKV010000350 DBNHKV010000000
VERSION     DBNHKV010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_687199"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /locus_tag="AC16PP_11200"
     CDS             complement(<1..1588)
                     /locus_tag="AC16PP_11200"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HIR6482238.1"
                     /translation="MADIFVSYASEDLDRVKPLITALEERRFAVWWDREIHPGTSWDE
                     AIERELESATWIVAIWTANSIRSRWVRTESMAALERDALIPILLDDVTLPVAFRTTQV
                     VDLRQWRPEGSDATFERLVSHLQGKPHPPAEVEAHRQRSPPNRRGPLLAALALTALAA
                     IALVWMVVTEPVRAPSDAGLAVTTFAGQPRIAVLPFDHDSDPEDRPFANGLTDEVIKG
                     LQQYRTIPVISRHASFALRDKGLSVQEIASQLGARYLVTGAVSRRGDRLRVSAGLTDA
                     DGHQLWKESYEVDFDPESVFALQDQIAEQIAGVSYPQILEGEIDRVARRDVTSLDAWG
                     HAVNAVEIINAMNVDRLDEGLLHAERALELDPNLPLAYWARAEIKVYQLVERGLTGQS
                     ADAMEDEILEDFQEALDISPFDGALCGCLGFVRVMRGEIEQAEAVFDTVLPVNPSNAI
                     LRTNYATLLTHQGKLEEARREAELAIRRDPLSRFGSMSWSTLSLVAAMDNDLEQAIQL
                     ARRALQVEGTETYGQVQLPML"
     gene            1820..2341
                     /locus_tag="AC16PP_11205"
     CDS             1820..2341
                     /locus_tag="AC16PP_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482239.1"
                     /translation="MKGKVLFFSLVFYAVVGMWLWGEYQQRPRVLDGLGQVMLGMTPA
                     QVVGVLGRPTEEFAPAEDEQRRLEYSYRSDGTSDYLVVFWPDEEGEPKADIICREGGS
                     VFGLAVGQSSELDIIDKLGEPSHRSFGPDGRAKAISYESFKVAFEIEGGWTREICVSQ
                     TGSISYALESITV"
     gene            2572..5319
                     /locus_tag="AC16PP_11210"
     CDS             2572..5319
                     /locus_tag="AC16PP_11210"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR6482240.1"
                     /translation="MRSVFRFMCFMLFVSAGPASLARGAVDVDQLAIPHETFVLENGL
                     TVIVHEDHSVPIVAVNLWYHVGSRNESRGRTGFAHLFEHFFFNGSEHYPHDFRRAMDD
                     VGANNRNGTTNPDRTNFFEDVPVSALERTLYLEADRMGFLAANITQETLDQERGIVQN
                     EKRQGENQPYGKVWNRIVESIYPYSHPYSWPIIGSMADLEAATLDDVKAWYAKYYGPS
                     NCVLSLAGDITVEQARELVGKYFAGIPPGPPVERYRQWVPTLERPLRDVMHDRVPQAR
                     IYRVYHTPGWLDAESVLIELNAGLLSGSRSAPLDRALVYDKQVATEVAAFAWDKEIAG
                     NLVVVVSVKPGVDPADAERELDAVMAQFLGQKPARSALDRARTRQVAQFIRGMERLGG
                     FGGRSDILAESMTYAGDPEAFLQRYRKVMEATPAELHAASTKWLDANHYTLTVLPFPE
                     LKAAAEDVDRTSLPALGPAPEVAFPEIGSRELDNGLRVLLLERRGAPLVNMSLAVDAG
                     YAADPAAAAGTASLALQLLEEGTRSRDGFALADDLDALGATISSASSLDLSFVQLRAL
                     KANLARSLAVFADVVRNPAFAGDMVALGKRRRLAQIAQEEAQPFSAALRMVPPLLYGD
                     GHVYAKPLTGSGYVETVRNIEPDDLSKWHSAWFRPNNATLIVTGDVTFDDLMPMVDDV
                     LGDWKPGAVPLKRVADVPRGKAGRIYLVDRPDASQSVIVAAQASIPGGQAEEVAIEAA
                     MRAFGGMSTSRFNQNLRIDKHWSYGVFGALLDARGHRPLLVVAPVQTDRTADSMAEIV
                     GEFRSILGDRPIAGEEFASLKRNMELRLPGRFETLASLEDAAHDLVNYGYSPAFYYDY
                     AKTVRALGEQDLAEAVNRVINPEQLIWVVVGDLEKIEAQVRALGFGEVVRLKASGASG
                     D"
     gene            5388..>6214
                     /locus_tag="AC16PP_11215"
     CDS             5388..>6214
                     /locus_tag="AC16PP_11215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6482241.1"
                     /translation="MADDYRERMQAYAQRLMGYKTGEMVSLMVHIGDELGLYRAMAGQ
                     DSVTAESLAEATGLHERWVAEWLRGQGAAEILDHVGDERFRLSDVAEQALLNEDSPAY
                     LQGFFSRPPSHEIVDRTIEAFSTGVGVSWGAHGDSASHFLCRTNRPFHMKLADEVIPL
                     LGGMSERLEAGGAVLDVGCGPGTALTELARAFPNSQFVGVDPAANMIAQAQQRYADMD
                     NVRFEVGYGETLEDVAAYDLVTTFDCMHDMTDPEGTMRAVRRAVKGDGAWLIKDIKA"
BASE COUNT         1060 a         1946 c         2052 g         1156 t
ORIGIN      
        1 gcagcatcgg cagctgcacc tgaccgtatg tttcggtgcc ctccacctga agcgcccgcc
       61 gggccagctg gatcgcctgc tcgaggtcgt tgtccatggc cgctaccagg ctcagggtag
      121 accaggacat ggaaccgaaa cggctgaggg ggtcccgtcg gatggccagc tcggcctcgc
      181 gccgggcctc ttccagcttg ccctgatgag tcagcagggt cgcgtagttc gtgcgcagga
      241 tggcgttgct cgggttgacg ggcagaacgg tatcgaacac cgcctcggcc tgttcgatct
      301 cgccgcgcat gacgcgcacg aaccccaggc agccgcacag cgcgccgtcg aacgggctga
      361 tgtccagggc ttcctggaag tcctcgagga tctcgtcctc catggcgtcc gccgactgcc
      421 cggtgaggcc gcgctcgacg agctgataca cctttatttc cgcccttgcc cagtacgcga
      481 gaggaaggtt cggatccagc tcgagagccc gctcggcatg gagtagtcct tcgtcgagcc
      541 gatcgacgtt catggcgttt atgatctcta ccgcgttcac ggcgtgcccc caggcatcca
      601 ggctggtgac gtcgcgcctc gctacccggt ctatttctcc ctcgagaatc tgcgggtagc
      661 tcacgccggc gatctgctcg gcgatctggt cctggagcgc gaaaaccgac tcggggtcga
      721 agtccacctc gtaggactcc ttccagagct ggtgcccgtc agcatcggtc aggccagcgc
      781 tgactctgag cctgtcgccc cggcggctga cggctccggt cacgaggtag cgcgcgccaa
      841 gctggctcgc gatctcctgg acggacagcc ccttgtcccg caacgcaaag ctggcatgcc
      901 gcgatatcac cgggatggtg cgatattgct gcagaccttt gatgacctcg tcggtcagtc
      961 cgttggcgaa cgggcgatcc tcggggtcgc tgtcgtggtc gaacggcaac acggctatcc
     1021 ttggttgccc ggcaaacgtg gtcacggcga ggcccgcgtc ggacggtgct cgaaccggtt
     1081 cggtcacgac catccagacc agggcaatgg cggcgagggc ggttagggcc agtgctgcga
     1141 ggagcgggcc gcgccggttt ggaggcgatc gttgtcggtg cgcctcgact tcggcgggcg
     1201 ggtgcggttt cccctggagg tgacttacca gcctttcgaa cgtcgcgtct gatccttccg
     1261 gccgccattg ccgcaggtcc actacctgag tcgtacggaa agccacggga agggtaacgt
     1321 cgtcgagaag aatcggtatg agcgcgtcgc gctcgagagc ggccatcgac tccgtgcgca
     1381 cccaccggga ccgtatggag ttggcggtcc agatcgccac gatccacgtt gccgactcga
     1441 gctcgcgttc gatcgcctcg tcccagctgg tgccggggtg gatctcgcgg tcccaccaca
     1501 cggcgaatct gcgttcctcc aacgcggtta tcagcggttt tacgcgatcg aggtcctcac
     1561 ttgcgtagga gacgaagatg tccgccatgc atcccgcttc cgcccaggtc cggccggcat
     1621 gatcaagtga atggaagcct agcagaatgt gcactcgtct tcccggacgg ccttttcgct
     1681 cccgcgtcca ctgggattgt gcgctgggtc acaaacttcc cgcgcctcag cgcaaggcgt
     1741 tttctcgatc taacgtgcag aatcttcagg cgcggtggcc tgtgccacgg attggctgct
     1801 caaggaaacg aatcccgcaa tgaaaggaaa ggtcctgttc ttctcgctgg tgttctacgc
     1861 cgtcgtcggt atgtggctgt ggggcgagta ccagcaaaga cctcgcgtcc tcgacgggtt
     1921 ggggcaggtg atgcttggca tgacgccggc gcaggtggtg ggtgttctgg gccggccgac
     1981 cgaggagttt gcccccgcgg aggacgaaca gcggcgcctc gagtactcct accgcagtga
     2041 tggtacgtcc gattacctcg tggttttctg gccggacgaa gagggcgagc cgaaggctga
     2101 catcatctgt cgggaaggcg gcagcgtctt cgggctggcc gtgggtcagt cttccgagct
     2161 cgacatcatc gacaagctcg gtgagccgtc ccatcgcagc ttcggcccgg acggccgcgc
     2221 aaaggccatc tcttacgaga gcttcaaggt tgccttcgag atcgagggcg gatggacgcg
     2281 ggagatctgc gtcagccaaa cgggctccat cagctacgcg ctggaatcga tcaccgtcta
     2341 gattcagcag cgggcactgg ctggacgcca gggagtcgca cagcggcagc atcgacctgg
     2401 acacgctgct ggcgaatttc acttcccttt tcgatcagcc gtttcccccg ggcggttgct
     2461 tccagtgatc cggatgcgat actcgcgctt cagcccggcg gaagtggcgc aggccggatg
     2521 gccggtgatc gccgtgttct cgcgagccat ggcgacaacg aggtgtgact gatgcgcagt
     2581 gtcttccggt tcatgtgttt tatgttgttt gtctcagcag gaccggcaag ccttgcccgg
     2641 ggcgccgttg acgttgacca gctggccata ccccacgaga cgttcgtgct cgaaaacggt
     2701 ctcaccgtga tcgtgcacga ggaccacagc gtgcccatcg tcgccgtgaa cctctggtac
     2761 cacgtgggat cccgtaacga aagccggggc cggacgggct ttgcgcacct cttcgagcac
     2821 ttcttcttca acggcagcga gcactacccg cacgatttcc gtcgcgccat ggacgacgtc
     2881 ggcgccaaca accgcaacgg cacgaccaac ccggaccgca cgaacttctt cgaggacgtg
     2941 cctgtctccg cgctcgagcg cacgctctat ctggaagccg accgcatggg ctttctcgcc
     3001 gccaacatca ctcaggagac tctggatcag gagcgcggca tcgttcagaa cgaaaagcgt
     3061 cagggcgaaa accagcccta tggaaaggtc tggaaccgca ttgtggagtc gatctacccc
     3121 tattcgcacc cctacagctg gcccatcatc ggcagcatgg ccgacctcga agcggccacg
     3181 ctcgacgatg tcaaagcctg gtacgccaag tactacggtc cgtccaactg cgtgctgtcc
     3241 ctggcgggcg acatcaccgt cgagcaggcc agggaactcg ttggcaagta cttcgcaggg
     3301 attcctccgg gtcctccggt ggagcgttat cgtcagtggg ttcccacgct ggagcggccg
     3361 ctgcgcgacg tgatgcacga ccgggttcct caggcacgca tctatcgcgt ctatcacacg
     3421 ccgggctggc tcgacgccga atccgtgttg atcgagctga acgcggggct gctcagcggc
     3481 agccgcagcg cgccgctgga tagggccctc gtctacgaca aacaggtggc cacggaggtc
     3541 gccgccttcg cctgggacaa ggagatcgcg ggcaacctcg tggtggtggt ctccgtcaag
     3601 cccggcgtgg acccggccga tgcggagcgg gagctagatg ccgtgatggc ccaattcctc
     3661 ggtcagaagc ctgcgcgaag tgctctcgat cgcgccagga cccgacaggt tgcgcagttc
     3721 atccgcggca tggagcgtct cggcggcttt ggcggccggt cggacattct ggccgaaagc
     3781 atgacctacg cgggcgatcc ggaagccttc ctgcagcgct accggaaagt gatggaggca
     3841 acgccggcgg agttgcacgc ggcttcgacg aaatggctcg atgcgaatca ctacacgctc
     3901 accgtgctgc cgttcccgga gctcaaagct gccgctgaag acgtggatcg cacctcgctg
     3961 ccagcgctgg ggccggctcc ggaggtcgcc tttcccgaga tcggttcccg cgagctcgac
     4021 aacggtttgc gggtgctgct cctcgagcgg cgcggcgcgc ctctcgtgaa catgtcgctg
     4081 gccgtggacg cggggtacgc ggccgacccg gccgccgcgg cgggcacggc ttcgcttgcg
     4141 cttcagctgc tggaagaggg cacccgatcc cgtgatggct ttgcgctggc cgacgatctc
     4201 gacgcgcttg gagccaccat cagctcggcc agctcccttg atctatcctt cgtgcagttg
     4261 cgcgcgctca aagcgaatct cgcccgttcg ctcgccgtgt ttgccgacgt ggttcgcaat
     4321 ccggcatttg ccggggacat ggtcgcgctt ggtaagcgtc gccggcttgc gcagatcgct
     4381 caggaggaag cgcagccgtt ctccgcagct ctgcgaatgg tgccgccgct tctgtacgga
     4441 gatggccacg tctacgccaa gccgcttacg ggatccggat atgtcgaaac ggtgcgcaac
     4501 atcgagccgg acgatctctc taaatggcac tccgcctggt tcaggcccaa caacgcgacg
     4561 ctgatcgtca ctggtgacgt gacctttgac gacctcatgc ccatggtgga tgacgtgtta
     4621 ggagactgga agcccggcgc cgttccgctc aagcgcgtcg cggacgtacc ccgtggcaaa
     4681 gcaggtcgca tctatctcgt cgatcggccg gacgcgtcgc aatcggtcat cgtggccgct
     4741 caggcgtcca ttcccggcgg gcaggcggag gaagtggcta tcgaggcggc gatgcgggcc
     4801 ttcggcggca tgtcgacctc ccggttcaat cagaacctca gaatcgacaa gcactggagc
     4861 tacggcgtgt tcggtgcgct gctcgatgcg cgcgggcacc gtccgctgct cgtggtggcg
     4921 ccggtgcaga ccgaccggac cgcggattcc atggccgaga tcgtcggtga gtttcgttcg
     4981 attctgggag atcggcccat tgccggagag gagtttgcga gcctcaaacg aaacatggag
     5041 ctgaggttgc ccgggcgttt cgaaacgctg gcgtcactgg aagacgcggc gcacgatctg
     5101 gtcaactacg ggtactcgcc ggccttctac tacgactacg ccaagaccgt gcgggcgctc
     5161 ggcgagcagg atctggccga ggcggtgaac cgggtgatta accctgagca gctcatctgg
     5221 gtggtagtcg gcgacctgga gaagatagag gcgcaggtgc gcgcactagg gttcggcgag
     5281 gtggtcaggc tcaaggcgtc gggtgcttcc ggcgactgag cgctggcttg ctgcgggatg
     5341 cggcggcgaa acgccgcggg gatacaacgg taacggggaa acttgccatg gcggacgatt
     5401 atcgggaacg gatgcaggcc tacgcccagc gtctgatggg ctacaagacc ggcgagatgg
     5461 tgtctctgat ggtgcacatt ggcgatgagc tcggccttta ccgggccatg gcgggacagg
     5521 acagcgtgac ggctgaatcc ctggccgagg ccaccggcct gcacgagcgg tgggttgccg
     5581 aatggctgcg cggccagggc gccgcggaga ttctcgatca cgttggcgac gagcggttca
     5641 ggctgtcgga cgtggccgag caggcgcttc tgaacgagga cagccccgcg tatctgcagg
     5701 gcttcttctc ccggccgccg tcccacgaga tcgtcgaccg gaccatcgag gctttcagca
     5761 cgggtgtggg ggtcagctgg ggcgcccacg gcgattcggc ctcccatttt ctctgccgga
     5821 ccaatcggcc tttccacatg aagctcgcag acgaggtgat ccccctgctg ggcggaatgt
     5881 cggaacgact cgaagccggt ggtgccgtcc tggacgtggg ttgcggtcct ggcacggcgc
     5941 tgaccgagct ggcaagagcc tttcccaact cgcagttcgt aggtgtcgat ccagcggcca
     6001 acatgatcgc tcaggctcag cagcgctatg ccgacatgga caacgtgcgc ttcgaggtgg
     6061 gctacggcga gacgctggag gacgtggcgg cctacgacct cgtcaccacc ttcgactgca
     6121 tgcacgacat gacggatccg gagggaacca tgcgggcggt gcgcagagct gttaagggag
     6181 atggcgcctg gctgatcaag gacatcaagg caca
//
LOCUS       DBNHKV010000351         4657 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_334666, whole genome shotgun sequence.
ACCESSION   DBNHKV010000351 DBNHKV010000000
VERSION     DBNHKV010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_334666"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..2730)
                     /gene="mutS"
                     /locus_tag="AC16PP_11220"
     CDS             complement(163..2730)
                     /gene="mutS"
                     /locus_tag="AC16PP_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR6482242.1"
                     /translation="MNTGSKTNHTPMMQQYLSIKARHPDDLLFYRMGDFYELFYEDAE
                     RAAELLDITLTARGQSAGKPIPMCGVPYHAADGYLARLVRQGVRVAICEQIGDPATSK
                     GPVAREVQRIVTPGTLTEEALQDARRDSILMAINPGPDEVYGLALLNLAAGRLQVAEV
                     RTRPLLAAEVARSDPSEILAVDELPEAVTGQRHLTRRDPLEFDTDLGRAKLTRHFATR
                     SLEGFDVPEDSPMVGAAAAVLEYAKSSQCRDLAFIDAMHRVAADDVVVLDPHSRRNLE
                     IDRRVDGSEENTLFALFNTTQTPMGARLLRSWLAGPSRNAHTVEARQASVESLLGADN
                     DNLRRLLKEVGDLERIVARVALGSATPRDLSRLRTGLGQVPDVKDFLQTLEAPRLADL
                     MARLPDFNDEVELLGTAIVEAPPATIRDGGVIASGYDAQLDSLRNLSDNAAGWLADLE
                     LRERERTGIATLKVGYNRVHGYYIETSRAAGNEVPAEYVRRQTLKNAERYITPELKQF
                     EDEALTSQARALKLEKQLYQNLLEQLGARAGGLRDAAAILAELDVLSCFAERASALRL
                     VRPEFSADPIIDIRQGRHPLVEQASAKPFVANDLSLGEKVRMLIVTGPNMGGKSTYMR
                     QSALIVLLAFAGSYVPAEAARLGPIDRIFTRIGAADDLAGGRSTFMVEMTETANILRN
                     ATPRSLVLLDEIGRGTSTYDGLALAWATAEHLARETGAFTLFATHYFELTELPEELSQ
                     CRNVHLTAAEHDGKIVFLHSVEPGPASQSYGVQVARLAGVPNEVIARARTRLQSLEQQ
                     QAERIAQQPDLFEGQSAEPEAPSPLEARLSELDVDGLSPREALALLYELKGLASPDS"
     gene            2864..3952
                     /gene="recA"
                     /locus_tag="AC16PP_11225"
     CDS             2864..3952
                     /gene="recA"
                     /locus_tag="AC16PP_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR6482243.1"
                     /translation="MANEQSNRKDSQADSQQDPQRSRALSAALAQIERQFGKGSMMRL
                     GDRELVAIPAIPTGSLGLDIALGIGGLPRGRVVEIYGPESSGKTTLTLQVIAEAQKGG
                     GTCAFIDAEHALDPIYADALGVDTDNLLVSQPDTGEQALEICDMIVRSGAVDVVVIDS
                     VAALTPKAEIEGEMGDAHVGLQARLMSQALRKMTGNIKQSNTLVIFINQIRMKIGVMF
                     GSPETTTGGNALKFYSSVRLDIRRIGAVKEGEEVVGNETRVKVVKNKVAPPFRQVEFQ
                     ILYGEGINHLGEVLELGVQQGIVEKSGAWYAYKSDRIGQGRKNACDYLESNPEIAAEI
                     EAQIREQLLPVRERSEDLVIPEPAQETL"
     gene            3985..4473
                     /locus_tag="AC16PP_11230"
     CDS             3985..4473
                     /locus_tag="AC16PP_11230"
                     /inference="COORDINATES: protein motif:HMM:NF046475.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR6482244.1"
                     /translation="MLIEHKAVQEPDSAGDEQFQVAFARGLKLLATREHSAAELRRKL
                     RVKGFSEALAGDAVAALQDQGLQSDRRFAEVFVRSRLSKGYGPVRIRRDLYQRGIGDD
                     VIDEELTFPAERWVELATAVRAKRFPESEPTEHGVWNVQARFLSRKGFPADIIYRVLG
                     QV"
BASE COUNT          790 a         1440 c         1552 g          875 t
ORIGIN      
        1 taccgggcat acctcaacgc agtctgtgag cttgcacttg atgcagtttt ccccaatgac
       61 gaacgtcatc tgtattgcct tcctgtcggg taagttggtg tttcgggtct gcgaagggac
      121 cgtattctaa ccatattcga tcccctgtcg agcgggcgcc cgtcagctat cgggggacgc
      181 caaacccttg agttcgtaga gcagcgcgag ggcttcccga gggctcaacc cgtctacgtc
      241 cagctcggac agcctcgctt cgaggggact cggcgcctcg ggttccgcgg attgtccctc
      301 gaagagatcg ggttgctgcg cgatacgctc tgcctgctgt tgctccagcg actgcaggcg
      361 agttcgcgcg cgggcgatga cctcgttggg cacgccggca agtcgggcca cctgcacacc
      421 gtagctctgg ctggcgggcc ctggctcgac gctgtgcagg aagacgatct tcccgtcgtg
      481 ctcagcagcg gtcagatgaa cgttgcggca ctgggaaagc tcctcgggca gctccgtcag
      541 ctcgaagtag tgcgttgcga acagggtgaa cgcgcccgtt tcccgggcca gatgctcggc
      601 ggttgcccag gccagtgcca gcccgtcata cgtgctggtc ccgcgaccga tctcgtcgag
      661 caggacgagg ctgcgcggcg tggcgtttct cagaatgttc gcggtttcgg tcatctccac
      721 catgaaggta gagcgaccgc cggcgaggtc gtcggccgcg ccgatgcggg tgaagatgcg
      781 gtcgatgggg cctagccgcg cggcttcggc cgggacgtag ctgccggcga aagcgagcag
      841 cacgatgagg gccgattgcc tcatgtacgt agacttgccg cccatgttgg gccccgtcac
      901 gatgagcatc cgtaccttct cacccagcga gaggtcgttg gctacgaagg gctttgcgct
      961 cgcctgctcg acgagcggat ggcggccctg cctgatgtcg atgatggggt ccgccgagaa
     1021 ctcgggtcgc accagcctca gggcgcttgc gcgctcggcg aagcacgaga gcacgtccag
     1081 ctccgcgagg atggccgcgg catcgcgcaa accgccggct cttgcgccca gctgctccag
     1141 caggttctgg tacagctgct tttcaagctt gagcgctcgt gcctggctgg tgagcgcttc
     1201 gtcctcgaac tgtttgagct ccggggtaat gtagcgctcg gcgtttttca gggtctgtct
     1261 gcggacgtac tcggccggaa cctcgttgcc ggccgcgcgg ctcgtctcga tgtagtagcc
     1321 gtggacccgg ttgtagccga ccttcagggt cgcgatgccc gtccgctccc gctcgcgcag
     1381 ctcgagatcg gccagccagc cggcggcgtt gtcactcaaa ttccgcaggc tatcgagttg
     1441 ggcgtcgtag ccggaggcga tcacgccgcc atctcgaatc gtggccggag gcgcctcgac
     1501 gatcgcggtc ccgagcagct cgacctcgtc attgaagtcg ggcaggcgag ccatcagatc
     1561 ggcgagccgg ggggcttcga gcgtctgcag gaagtctttc acgtccggca cctgtcccag
     1621 gcccgtgcgc agccgtgaca ggtcgcgcgg cgtcgccgaa cccagcgcca cccgggcgac
     1681 gattcgctcc aggtcgccga cctccttgag cagtcggcgc aggttgtcgt tgtccgcacc
     1741 cagcaggctt tcgaccgagg cctggcgcgc ttcgacggtg tgggcgttgc ggctgggccc
     1801 ggcgagccag cttcgcagca ggcgtgctcc catgggcgtt tgcgtcgtgt tgaacagagc
     1861 gaaaagcgtg ttctcctcgc tgccgtccac gcgccgatcg atttccagat tgcgccgtga
     1921 gtgcggatcc agcacgacca catcgtccgc agccacgcga tgcatggcgt cgatgaacgc
     1981 cagatcgcga cactgcgagg actttgcgta ctcgagcacg gcggccgcgg cgcccaccat
     2041 gggcgagtcc tcggggacgt cgaagccctc cagggagcgc gtcgcgaagt gtctggtcag
     2101 cttggcccgg ccgagatcgg tgtcgaactc cagcggatcg cgccgcgtca ggtggcgctg
     2161 tccggtgacc gcttcgggca actcgtcgac ggccaagatc tcgctcggat cgctgcgggc
     2221 cacctcggct gccagcagcg ggcgggtccg cacctctgcg acctgcagcc tgccggccgc
     2281 aaggttcaac agcgccaggc cgtagacctc gtccgggccc ggattgatgg ccatgaggat
     2341 gctgtcgcgt cgggcgtcct ggagagcctc ctcggtgagg gtgccggggg tgactattct
     2401 ctggacctct ctggccaccg gtcccttgct ggtggccggg tcgccgatct gctcgcagat
     2461 ggccacccgc acgccctggc gcaccaaccg ggcgaggtac ccgtcagccg cgtggtaggg
     2521 cacgccacac atgggtatcg gcttgcccgc cgactggcca cgggcagtga gcgtgatgtc
     2581 cagcagctcg gccgcgcgct cggcatcctc gtagaaaagc tcgtagaaat cgcccatgcg
     2641 gtagaacagc aggtcgtccg gatgccgggc cttgatgctc aggtactgct gcatcatcgg
     2701 cgtgtggttg gtcttggatc ccgtgttcat gaacctatta ttctctgtgg ctccgagatg
     2761 tctcaagggc atgtgcgaga acgtatagaa gggcccttcg agcggttgcc atcaatactg
     2821 attatatata cagtaccgtc cgttaacccc gcaggagaga ttcatggcca acgagcagtc
     2881 caaccgcaaa gattcgcagg cggattccca gcaggatccg cagagatccc gggcgctgtc
     2941 cgcggcgctg gcgcagatcg agcgccagtt cggcaaaggc tcgatgatgc ggcttggaga
     3001 tcgggagctg gtcgccatac cggcgatccc aacgggctcg ctggggctag acattgctct
     3061 gggtatcggc ggtctgccaa ggggccgggt ggtcgagatc tacggtccgg agtcgtcagg
     3121 taagacgacg ctcaccctcc aggtcattgc cgaggctcaa aagggcggcg gcacctgcgc
     3181 tttcatcgac gcggagcacg ccctcgaccc catctacgcc gatgcgctcg gtgtcgatac
     3241 ggacaatctg ctggtatccc agccggatac cggcgagcag gctctggaaa tctgcgacat
     3301 gatcgtccgt tccggagccg tggacgtggt ggtcatcgac tccgtggcgg cactgacccc
     3361 caaggcggag atcgaagggg aaatggggga tgcccacgtc ggcctgcagg ctcgtctgat
     3421 gagccaggcg cttcgaaaga tgacgggcaa catcaagcag tcgaacaccc tggtcatctt
     3481 catcaatcag attcgtatga agatcggggt catgttcggt tctccggaaa ccaccaccgg
     3541 cgggaacgcg ctgaaatttt actcgtcggt gcggctggac atccgccgga tcggcgccgt
     3601 gaaggaaggc gaagaggtgg tcggcaacga gactcgggtc aaagtggtca aaaacaaggt
     3661 ggctccgccc ttcaggcagg tcgagttcca gatcctttac ggggagggca tcaatcacct
     3721 cggtgaggtc ctggagttgg gtgtgcagca gggcatcgtc gagaaatcgg gtgcctggta
     3781 tgcctacaag tccgatcgga tcgggcaggg acggaagaac gcctgcgact acctcgagtc
     3841 caatcctgaa atcgcggccg agatcgaagc ccagattcgc gagcagctcc tgcccgttag
     3901 agagcgttcc gaggatctcg tcattcccga gccggcccag gaaacgctgt agacgaatca
     3961 ggccccgggg agcgcggtgg tcgcatgttg attgagcaca aggcggttca ggagccggac
     4021 agcgcgggcg atgagcagtt ccaggtggcg tttgcccgag gcctcaagct gctcgccacc
     4081 cgggagcatt cggcggccga gcttcgacgc aagcttcgcg tcaaaggatt ttctgaggcg
     4141 ttggccggag acgccgtcgc tgcgctgcag gaccaggggc tgcaatccga ccggcgcttc
     4201 gcggaggtgt ttgttcgcag tcgtctcagc aaggggtacg ggcccgtccg aattcgtcgg
     4261 gatctatatc aacgcggcat cggcgatgac gtgattgacg aggagctgac cttcccggcg
     4321 gaacgctggg tcgaactcgc cactgcggtt cgggccaaac ggtttccgga aagcgagccc
     4381 acagagcacg gcgtctggaa tgtgcaggcg cgttttctgt cccgcaaggg gtttccggcc
     4441 gacatcattt accgggtgct ggggcaggtc taggcgccca atctcgcggc cgcggaggcc
     4501 ctgtccgtca ccccgtggca gaccaggcgg ccatgtcaat tggacgcggt ttccgtatac
     4561 tgcgcgcctc ctaattggtt gattccaaag atgaacaccg ctgaaatccg ttcagcattc
     4621 ctgtcgttct tcgccgagcg ggagcaccgc gtcgtgc
//
LOCUS       DBNHKV010000352         2268 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1395302, whole genome shotgun sequence.
ACCESSION   DBNHKV010000352 DBNHKV010000000
VERSION     DBNHKV010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1395302"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2259
                     /gene="cofH"
                     /locus_tag="AC16PP_11235"
     CDS             <1..2259
                     /gene="cofH"
                     /locus_tag="AC16PP_11235"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: protein motif:HMM:NF005609.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HIR6482245.1"
                     /translation="CTFAQVPRKLKAPYMSVDEVLEIARHGAAMGCKEALFTLGEKPE
                     LRYRAAREALAELGFETTLDYLAHVAEIVYRETGLLPHLNPGTMTPEELARLRPLSPS
                     MGIMLESTAERLGEKGMPHHGSPDKVPAVRLETLDHAGAARVPFTTGILIGIGETRRE
                     RIESLLAIREVHERHGHVQEIIVQNFRAKPETKMARAPEPDLNELLWTIAVTRILFGS
                     AMSVQAPPNLSPGVLAQIVAAGINDWGGVSPLTPDFVNPEAPWPHLDDLARETAAAGK
                     DLFERLTVYPGYVRDLATWVAPELQERVLELSDSEGFARTSQWCPGAEVEPPARLLES
                     VTRPPERVSADLERILRKAGDGRRLEASEVVRLFQARGEDFSAVVQAADGLRRETNGD
                     VVSFCVNRNINYTNICYFKCQFCAFSKGKLSENLRGRPYDLSHEEIARRTQEAWSRGA
                     TEVCMQGGIHPEYTGETYVGILQAVKEAVPEMHIHAFSPLEVWQGAATLGLDLDTYLE
                     RLKAAGLGTLPGTAAEILDDEIRQIICPDKINTEQWLDVMRAAHRVGFKTTATIMYGH
                     VESAVNWARHLLRIRDLQAETGGFTEFVPLPFVHMEAPMYLKGKARKGPTFREAILMH
                     AVARLTLHPLIPNIQASWVKMGHAGVQACLQAGCNDLGGTLMNESISRAAGTTHGQET
                     SPQRMAEMIKAVGRIPRQRTTLYEDASEERIAVGLAAGELTEIVNTPARKYERKRSGP
                     LLRPGLIDAVEV"
BASE COUNT          420 a          756 c          752 g          340 t
ORIGIN      
        1 tgcacgttcg cccaggtccc gcggaagctc aaagcgccct acatgagcgt ggacgaggtg
       61 ctcgaaatcg cccgtcacgg cgccgccatg ggttgcaagg aagccctgtt caccctaggc
      121 gagaaacccg agctgcgtta ccgagcagcc cgggaagcgc ttgccgaact gggtttcgag
      181 accacgctcg actatctggc ccacgtggcg gagatcgtct atcgggagac cggcctgctg
      241 ccgcacctca accccggcac catgaccccc gaggagctcg cccggctgcg ccccctctcg
      301 ccatccatgg gcatcatgct ggaatccaca gccgagcggc tgggcgagaa agggatgcct
      361 caccacggct cgccggacaa ggtgcccgcc gtgcgtctcg agaccctcga ccacgccggc
      421 gccgcgcggg ttccgttcac caccggcatt ctcatcggta tcggcgagac ccgccgggag
      481 cgcatcgaat cgcttctggc catccgcgag gtgcacgagc gccacggcca cgtgcaggag
      541 atcatcgtcc agaacttccg ggccaagccc gagaccaaga tggcccgcgc acccgagccc
      601 gacctcaacg agctgctctg gaccatcgcg gtgacccgca tcctgttcgg gtccgccatg
      661 agcgtgcagg cgcccccgaa cttgagcccg ggggtgctcg cacagatcgt ggcggccggc
      721 atcaacgact ggggcggcgt ctcgccgctg acgccggatt tcgtcaaccc ggaagcgccg
      781 tggccgcacc tggacgatct cgcaagagag acggcggcgg ccggcaaaga ccttttcgaa
      841 cgcctgacgg tctatccggg ctacgtccgg gatctcgcca cgtgggttgc acccgagctg
      901 caggagcgcg tgctggagct ctccgacagc gaaggctttg cccgcaccag ccagtggtgt
      961 ccgggtgccg aggtggagcc accggcgagg ctgctcgagt ccgtaacgcg gccacccgag
     1021 cgcgtctcgg cggatctgga acgtattctc cgcaaagcgg gcgatggccg gcggctggag
     1081 gcgtccgagg tggtgcgtct gtttcaagcg cggggcgagg acttcagcgc cgtggttcag
     1141 gcagccgacg ggctgcgccg ggagacgaac ggcgacgtgg tgagcttctg cgtcaaccgc
     1201 aacatcaact acaccaacat ctgctacttc aagtgccagt tctgcgcgtt ctccaagggc
     1261 aagctgtcgg agaatcttcg cgggcgtccc tacgacctgt cccacgagga gattgcccgc
     1321 cgtacccagg aagcatggtc ccggggcgct acggaagtgt gcatgcaggg cggcatacac
     1381 cccgaataca cgggtgagac ttatgtgggc atcctccaag cggtcaagga agccgtccct
     1441 gagatgcaca tccacgcgtt ttccccgctg gaggtctggc agggcgcagc aacgctgggg
     1501 ctggatctgg atacgtacct cgaacgcctc aaggcggccg gcctgggtac cctgcccgga
     1561 acggcggcgg agatcctcga cgatgagatc cggcagatca tctgcccgga caagatcaac
     1621 accgagcagt ggctggacgt catgcgcgcg gcccatcggg tagggttcaa gaccacggcg
     1681 accatcatgt acgggcacgt ggagagtgcc gtcaactggg cccggcacct gctgcggatc
     1741 cgcgatctgc aggcggaaac cggcggtttc acggagttcg tgccgctccc gttcgtccac
     1801 atggaagcgc ccatgtactt gaagggcaaa gcccgaaagg gtccaacctt ccgcgaggcg
     1861 atcctgatgc acgccgtggc gcgcctgacc ctgcacccgc tgatcccaaa cattcaggcg
     1921 agctgggtga agatgggcca cgcgggcgtg caggcgtgcc tgcaggccgg atgcaacgat
     1981 ctgggcggca ccctgatgaa cgagagcatc agccgggcgg cagggaccac ccacggtcag
     2041 gaaacgtcac cgcagcgaat ggcggagatg atcaaggcgg tggggcgaat cccgcggcag
     2101 cgaacgaccc tttacgagga cgccagcgaa gagcgcatcg cggtgggcct tgcggcgggc
     2161 gagctcaccg agatcgtcaa cacgccggcg cggaagtacg agcgcaagcg atcgggcccc
     2221 ctcctccgtc cgggtctcat cgacgcggtc gaggtctaat cctgaccc
//
LOCUS       DBNHKV010000353         8195 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_122884, whole genome shotgun sequence.
ACCESSION   DBNHKV010000353 DBNHKV010000000
VERSION     DBNHKV010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_122884"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..842
                     /gene="tolA"
                     /locus_tag="AC16PP_11240"
     CDS             123..842
                     /gene="tolA"
                     /locus_tag="AC16PP_11240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HIR6482246.1"
                     /translation="MPAVRDYALPLLLALVLHALAFLSLRAGWNPETAEVRAIKPQIV
                     RSTLVMMEPKAKPVPRPKAAAKASPLPARQLEEPKPKAEARPADPPQAPPPDVGAERR
                     RAAQAERQERLEQLAEASFLDALETEAADLAESLAAEEAEAVAQTFRYGIYERVVANW
                     SRPPSARNNMQARLLVELVPTGDVVSVTLIEGSGNEAFDRSAEAAVRKARRFEVPKDS
                     DVFERYFRRFTLLFRPEDLLR"
     gene            860..2173
                     /gene="tolB"
                     /locus_tag="AC16PP_11245"
     CDS             860..2173
                     /gene="tolB"
                     /locus_tag="AC16PP_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003086128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HIR6482247.1"
                     /translation="MSALVCLVLMLTSAATLAAESLDTIIIDAGYDKQTRIAVVPFRQ
                     GPEFSDRQQMGDIIGFDLVRSGQFSPLGMENMLSFPTTREEVFFRDWRVLGIEYLVIG
                     RTGVDAAGNLEVTYELFDVFNERRMTGGTTTALRSQWRDVAHRVADEVFEAITGIPGA
                     FSTRILYVLAQNVGSPDEAFRLEVADSDGERSRTLFSSTQPLLSASWSPDGTRVAYVS
                     FEGGRPSIVLQNVATGARERLTQFSGINGSPVFSPDGKRLALVLSRDGNPEIYIMDLD
                     DRRLRRITTHHAIDTEPSWTPDGKGVIFTSDRGGRPQIYQLDLATNFIERLTFQGDYN
                     ARARLLPDGKHLIFVHRRQGVFHIAWQDLERDDVRVLTQTSLDESPSLAPNGTMLIYA
                     TQDRGRGILAVVSIDGRVKYRLPSSSGDVREPAWSPYLDVLERVD"
     gene            2235..2777
                     /gene="pal"
                     /locus_tag="AC16PP_11250"
     CDS             2235..2777
                     /gene="pal"
                     /locus_tag="AC16PP_11250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HIR6482248.1"
                     /translation="MTTRWFRTLLAIFFFGAVLAGCATGGQPAPAEPVAEPVETVDTT
                     PPPAPPQPTPPPAFDPNGNPFAPGTQRLLSRTFYFEYDRAVINPDDLASLELHASILR
                     ENPDRKIVIEGHCDERGTREYNLALGERRADAMRSFLTSAGVSRRQIETVSYGEERPE
                     DPGHDEGAWARNRRAIMIYR"
     gene            2877..3542
                     /gene="ybgF"
                     /locus_tag="AC16PP_11255"
     CDS             2877..3542
                     /gene="ybgF"
                     /locus_tag="AC16PP_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HIR6482249.1"
                     /translation="MTTETQSSAVPAPAGDASEQRRLAELFYQLQVLRQEVQDLRGLV
                     EEQSYQLNRLAKDQQEQYLDLDRRVAALRAQAPEPTLAETSPPPGGPVPGGVPGSERD
                     AYTAAFNQMKNRQFDEAVASFNALVTDYPNGQFTPNAFYWLGELHLAQADDEMARESF
                     SQVINLYPDHQKAPDALYKLGIVHHRLGDTANALQYLERVTAEYPQSSAAGLARTYSA
                     EIR"
     gene            3539..4198
                     /gene="queE"
                     /locus_tag="AC16PP_11260"
     CDS             3539..4198
                     /gene="queE"
                     /locus_tag="AC16PP_11260"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HIR6482250.1"
                     /translation="MTEQATRQLRISEIFLSLQGETSTVGLPTVFVRLTGCPLRCHYC
                     DTAYAFSGGEVMTLEDILARVRDLGVRRVTVTGGEPLAQPDCLGLLTSLCDEGFAVSL
                     ETSGALDVAGVDERVVKIIDVKTPGSGEVSRNRWENLAHCADGDQFKFVICHREDYEW
                     ARLKCEEHKLFEGACEVLFSPSSAELPARDLADWIVRDRLPVRMQIQLHKYLWGDQPG
                     R"
     gene            4195..4863
                     /gene="queC"
                     /locus_tag="AC16PP_11265"
     CDS             4195..4863
                     /gene="queC"
                     /locus_tag="AC16PP_11265"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HIR6482251.1"
                     /translation="MKRAIVLLSGGLDSATTLAVAKDEGFDCYALSIDYGQRHGAELA
                     AAREVARRAGVIEHRVIPVDVSVFGGSALTDRSIDVPEQPSEGIPITYVPARNTVFLS
                     LALAWAETLGLRDLFIGVNAVDYSGYPDCRPAFIFAFENLANLATKAGVEGDWYHVHA
                     PLIELSKAGIVELGISLGVDFGITVSCYQPDDDAAACGRCDSCRLRAQGFEEAGVEDP
                     TRYR"
     gene            complement(4854..5657)
                     /locus_tag="AC16PP_11270"
     CDS             complement(4854..5657)
                     /locus_tag="AC16PP_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482252.1"
                     /translation="MDDTNLTARQLILALLDSAPAPQIQAASLIAAGALFGMDPGAIR
                     VATARLVRDRVLESTARGQYTVGGGGAGLHRTVLSWADLEETLKPWAGEWLAVYLGSL
                     KRTNKTLVRARERALQLKGFVTVDPGLAVRPANLKASLPELLEDLRLLGLDPSAGLYR
                     IAETADGSRFAGLWDTDGLARRYQRHIDEIARSTRGIVELSPRAAAKETLLVGRAAIR
                     DILRDPLLPAELVDSRLRRELVHRMKKYDRLGKSCWRAFYADVGKADQR"
     gene            5756..6643
                     /locus_tag="AC16PP_11275"
     CDS             5756..6643
                     /locus_tag="AC16PP_11275"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HIR6482253.1"
                     /translation="MPRIEDYLTPEQVEACLRRSDWQAARVLGVNYLMIVAAFALSIA
                     WPNPLTIVLSVLILGGRQLGMEVLVHDCAHSVFFARRRVNEIVGHWLCGGLVNTNLYR
                     YRDYHLGHHRYAGTDKDPDLYLAQMYPAPRDSMRRKLLRDVTGQTGIRDTWRQLRVMK
                     PGRNAPFLVLHGMMIAALAAVGALWAYLLWWAAYVFVYPLLTRVRYMGEHGVCFDYAS
                     ADPRENTSSTVASWWERLLVAPNRVNFHLEHHLLASVPLYHLPKMQRWLKESGFYGDK
                     DCFSHGYLDVVRKATVPAA"
     gene            6742..>8195
                     /locus_tag="AC16PP_11280"
     CDS             6742..>8195
                     /locus_tag="AC16PP_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6482254.1"
                     /translation="MSFKMQQFDMVIVGAGVSGIGAACHFREKCPGKTFAILESRDAI
                     GGTWDLFRYPGIRSDSDMHTFGYNFKPWRNPKAIADGPSIREYVREAAREHDVDPHIR
                     FNHRLRSARWSSDSATWTLEVETPDGVVEFSCNFLFMCGGYYSYAGGYTPDFPGMADF
                     EGPVVHPQQWPDELDYSSKRVLIIGSGATAVTLLPAIAERAAHVTMLQRSPTYMVSRP
                     DRDAIANRLRRIFPEKVAYAITRWKNTTLQQFFYRRTRVNPEWVKDRLLRGVRAQLGP
                     DFDVQTHFTPSYNPWDQRLCLVPNGDFFRALRQGKATVVTDLIESFTSSGVRLKSGQT
                     IEADVIVTATGLNLEVMSGVEFWVDEAKVNFPDTFTYKGMMFSGVPNLVQTFGYINAS
                     WTLRADLTSEYVCRLVNRMDELGVSQVAAQLREQDRDMPGKQWIDDFTPGYMQRSMHL
                     FPKQGEKDPWRNTQNYAQDKKAIRHAPLEDGVLT"
BASE COUNT         1414 a         2633 c         2672 g         1476 t
ORIGIN      
        1 aggccgatca caagctggac tacggagccg tggtccgggt gatgaccgtc ctgcagcggg
       61 tgggcgcgca gagcgtcggt ctgattaccg acccgcccga gcttgccggg gagtgacgag
      121 caatgccggc cgtccgtgac tacgcgctgc cgctgctgct ggcgctggta ctgcacgctc
      181 tggcctttct ctctctccgg gccggctgga accccgagac cgccgaggtg cgcgccatca
      241 agccgcagat cgtgcggtcg acgctggtca tgatggagcc caaggcaaaa cccgtcccga
      301 gaccgaaagc cgccgccaag gcctcacccc tgcctgcccg gcagctcgaa gagccgaagc
      361 ccaaggcgga agcgcgacct gcggaccccc cccaggcgcc tccgccagac gtcggggccg
      421 agcgcagacg agccgcacag gccgagcggc aggagcgtct cgagcagctc gccgaagcct
      481 cgtttctgga cgccctggag accgaggccg ccgatctcgc cgagagcctg gctgcggagg
      541 aggccgaagc cgtcgcgcag accttccgtt acgggatcta cgaacgcgtg gtcgccaact
      601 ggtcgcggcc acccagcgcg cgaaacaaca tgcaagcccg tttgctcgtc gagctcgtgc
      661 cgactggcga cgtcgtttcg gtaacgctca tcgaggggag cggcaacgag gcattcgacc
      721 gctcggccga ggcggccgtg cgaaaagcac ggcgtttcga ggtgccgaaa gattcggacg
      781 tcttcgaacg ctacttccgt cgtttcacac tgcttttccg tccggaggac ctgctgcgtt
      841 gattaacttt gcccgctgcc tgtccgcgct cgtctgcctg gtcttgatgc tgacgtcggc
      901 cgcgaccctt gccgcggaga gcctggacac catcatcatc gacgcggggt acgacaagca
      961 gacgcgcatc gccgtggtgc cgttccggca ggggccggag ttttccgatc gtcagcagat
     1021 gggtgacatc atcgggttcg accttgttcg cagcggccag ttttcgcccc ttggcatgga
     1081 gaacatgctg tcgtttccca ccaccaggga agaagtcttc ttccgcgact ggcgcgtgct
     1141 cggcatcgag tatcttgtca tagggcgcac gggcgtggac gccgccggca atctcgaggt
     1201 cacctacgag ctgttcgacg tgttcaacga gcgtcggatg acgggcggca cgaccacggc
     1261 gctgcgcagc cagtggcggg acgtggccca tcgcgtcgcc gacgaggtct tcgaggcgat
     1321 caccggcatt cccggcgcgt tctccacccg cattctgtac gttctcgcgc agaacgtcgg
     1381 gtccccggac gaggcgtttc ggctggaggt ggccgactcg gacggcgagc ggtcgcggac
     1441 gctgttctcg tccacccaac cactcctttc ggccagctgg tcgcccgatg ggacccgcgt
     1501 ggcctacgta tccttcgagg gaggacggcc gtccatcgtg ctgcagaacg tcgccacggg
     1561 ggcgcgggag cggctgaccc agttttcggg aatcaacggt tcccccgtct tctcgccgga
     1621 tggcaagcgg ctcgctctgg tgctctcgcg ggacggcaac cccgagatct atatcatgga
     1681 tctcgacgat cggcgcctgc gccgcatcac cacccaccac gccatcgaca ccgagcccag
     1741 ctggacgccg gatggcaagg gcgtgatatt cacctcggac cgcggcggca ggccacagat
     1801 ctaccagctc gacctcgcga ccaacttcat agagcggctc acgtttcagg gtgactacaa
     1861 tgcccgggcg cgcctcctgc cggacggcaa gcaccttatt ttcgtacacc gtcgacaggg
     1921 ggtgtttcac atagcctggc aggacctcga gcgcgacgac gtcagggtgc tcacccagac
     1981 gtccctggac gagtctccgt cgcttgcgcc caacggcacg atgctaatct atgcgacgca
     2041 ggatcggggg cggggtatac tcgccgtcgt ttcgatcgac ggccgcgtga agtacagatt
     2101 gccctcttcc tcgggtgatg ttcgggagcc ggcctggtcc ccttatctcg atgttctgga
     2161 gagggtggac tgaacgggct ccgagcctgg cagttctcag cagccgtgga accccagcaa
     2221 cacaggagat acagatgaca acacggtggt ttcggactct ccttgcgatt ttctttttcg
     2281 gggcggttct ggcgggctgc gcaaccggag gccagcccgc tccagcggaa cccgtggccg
     2341 agcctgtcga gacggtggac acgaccccgc cgccggcccc accccagcct acgccgccgc
     2401 cggccttcga tccgaacggc aatcctttcg cgccgggaac ccagcggctc ctgtcgcgga
     2461 cgttctactt cgagtacgac cgtgcggtca tcaacccgga cgacctggct tccctggagc
     2521 tgcacgcttc catcttaagg gagaacccgg accgcaagat cgtcatcgag gggcattgtg
     2581 acgagcgcgg cacccgggag tacaacctgg cgctcggcga acgccgggcg gacgcgatgc
     2641 gaagttttct gacctcggcg ggtgtctctc gacgccagat cgaaaccgtg agctacgggg
     2701 aagagcgccc cgaggacccg ggccacgacg agggcgcctg ggcgcggaat cgccgggcca
     2761 tcatgatcta ccgctagacc gtggtgacgt caccgccaaa acaccgtctg cggctggtgc
     2821 tgaagctggc cctcgccggg tttctaggtt cggtcgtcgg cccggtcgcg gccgagatga
     2881 cgaccgaaac ccagagttcc gccgtgcctg cgccggccgg ggacgccagc gagcagcgcc
     2941 ggctggccga gctcttctat cagctgcagg ttctcaggca ggaggtccag gatctccggg
     3001 gcctggtgga agagcagagc taccagctga accggctcgc caaggatcag caggagcagt
     3061 acctcgatct ggatcgccgg gtggccgcgc tgcgcgcgca agcgcccgaa cccaccctgg
     3121 ccgagacgtc gccgccaccc ggcggcccgg tgccgggcgg cgtgcccggc tcggaacgcg
     3181 atgcttacac ggccgccttc aatcagatga agaaccggca gttcgacgag gcggtggcgt
     3241 cgttcaacgc gctggtcacg gattatccca atggtcagtt cacgcccaac gctttttact
     3301 ggcttggcga gcttcatctg gcccaggccg acgacgagat ggcgagggaa tccttctccc
     3361 aggtgatcaa cctgtacccc gatcaccaga aggcgccgga tgccctctac aagctgggca
     3421 tagttcacca ccggctcggt gacacggcga atgcgctgca gtatctcgag cgcgtgaccg
     3481 ccgagtaccc gcaaagctcg gcggccggcc tcgcccggac ctactcggcg gagattcggt
     3541 gaccgaacag gcaacgcgcc agcttcgcat atcggaaatt tttctgtccc tccaggggga
     3601 gaccagcacc gttggcctgc ccacggtctt cgtccgcctc accgggtgtc cgctgcggtg
     3661 ccattactgc gacacggcgt acgccttcag cgggggcgag gtgatgactc tggaagacat
     3721 cctggcccgt gtgcgtgacc tgggtgtccg ccgggtcacg gtgaccggcg gagaaccgct
     3781 ggctcagccc gattgtctcg gtcttctcac ctccctgtgc gacgagggat ttgcggtctc
     3841 tctggagacc agcggtgcgc tggacgtggc cggcgtggac gagcgcgtcg tgaagatcat
     3901 cgacgtcaaa acgccgggat ccggcgaggt gtcccgtaac cgatgggaaa atcttgcgca
     3961 ttgcgcggac ggggatcagt tcaagttcgt gatctgtcat cgtgaggact acgagtgggc
     4021 gaggctcaag tgcgaggagc acaagctgtt cgaaggcgct tgcgaagtgc tgttctcgcc
     4081 gagcagcgcg gagctgccag cccgtgacct ggctgattgg atcgtccggg accggcttcc
     4141 ggtgaggatg cagatccagc tgcacaaata cctgtgggga gatcaaccgg gaagatgaag
     4201 cgcgccatcg tgctcctgtc gggcgggctg gattccgcca ctacgcttgc cgtggccaag
     4261 gacgagggct tcgactgcta cgcgctctcg atcgactacg gccagcggca tggggcggag
     4321 ctcgctgcgg ccagggaagt cgcgcgacga gcgggggtga tcgagcaccg cgtgatcccg
     4381 gtggacgttt ccgtcttcgg cggatctgcg ctcaccgaca gatcaatcga cgtgcccgaa
     4441 cagcccagcg agggcattcc catcacctac gtgccggccc ggaacaccgt gttcctctcg
     4501 ctagcgctgg cttgggctga aacgctcggg ctgcgcgacc ttttcatcgg cgtcaacgcc
     4561 gtagattact caggctatcc cgattgccgt ccggccttca tcttcgcctt cgagaacctt
     4621 gccaacctgg ccaccaaggc cggggtggag ggcgattggt atcatgttca cgcgccgctc
     4681 atcgagctca gcaaagccgg gatcgtggag ctggggatct cgctcggcgt cgacttcggc
     4741 atcacggtgt cctgttatca gccggacgat gacgcagccg catgtggcag gtgcgacagc
     4801 tgccgtcttc gtgcccaggg attcgaggaa gccggggtgg aggatccgac ccgttaccgc
     4861 tgatcggctt tgccgacgtc cgcgtagaac gcccgccagc aggacttccc gagacggtcg
     4921 tactttttca tccggtgcac cagttcccgc cgcagacggc tgtcgacgag ctccgcgggc
     4981 agcagcgggt cccggaggat gtccctgatg gcggcgcgcc cgacgagcag cgtttccttc
     5041 gcggctgcgc ggggagacag ctcgactatg cctcgggtgc tgcgggcgat ctcgtcgatg
     5101 tggcgctggt agcgcctggc gagaccgtcg gtgtcccaaa gcccagcaaa gcgggagccg
     5161 tctgccgttt ccgcgatgcg atagagaccg gcggacgggt cgagaccgag caggcgcagg
     5221 tcctcgagca actcgggcag ggaggctttg agattggcgg ggcgaaccgc cagccctggg
     5281 tcgacagtga cgaagccttt gagctgcaat gctcgctcac gggcgcgaac cagggtcttg
     5341 ttggtgcgct tcaggctgcc gagatagacg gcaagccatt ccccggccca gggtttgagc
     5401 gtttcttcca gatccgccca cgagagcacg gtccggtgga ggccggctcc gcccccgccc
     5461 acggtgtact gcccccgcgc cgtgctctcg agaacccgat cgcggaccag acgcgccgtc
     5521 gccacccgaa tggcgcccgg atccataccg aagagcgcgc cggccgcgat cagcgatgcg
     5581 gcctggatct gcggcgcagg cgctgaatcg agcagggcga ggatcagctg cctggcggtg
     5641 aggttggtat cgtccatgcc gcttataaat aacaaatttt gactattaag gtcaaaacgt
     5701 gtaataaata ccgccatgcc gtggtgaaac ggcgaggagg tgcccgttgg ctgacgtgcc
     5761 gcgtatcgaa gattatctga cgcccgagca ggtcgaggcg tgcctgcgcc gatctgactg
     5821 gcaggctgcc cgcgtgcttg gcgtgaatta tctgatgatc gtcgccgcgt tcgcgctttc
     5881 catagcctgg ccaaacccgc tgaccatcgt gttgagcgtg ctgattctgg gcgggcggca
     5941 gctgggtatg gaagtgctcg tgcacgattg cgcccacagc gtgttcttcg cgcggcgcag
     6001 ggtcaacgag atcgtggggc actggctgtg cggcgggctg gtcaatacca acctttatcg
     6061 ctaccgggac tatcacctcg gccatcaccg ttacgcgggc acggacaagg atcccgacct
     6121 gtacctggcg cagatgtatc cggccccgcg cgacagcatg cgtcgcaagc tgcttcgcga
     6181 cgtcactggg cagacgggca ttcgtgacac gtggcggcag ctgcgggtga tgaagccggg
     6241 tcgcaacgcg ccgttcctgg ttcttcatgg gatgatgatc gcggctctcg ccgccgtggg
     6301 ggccttgtgg gcgtacctgc tctggtgggc ggcctacgtc ttcgtgtacc cgctgctgac
     6361 gcgcgtacgc tacatgggtg agcacggtgt gtgctttgac tatgcgagcg ccgacccccg
     6421 cgagaacacg agcagcacgg tggcgtcctg gtgggaacgc ctgctggtcg ctccgaatcg
     6481 cgtgaacttc catctcgagc accacctgct tgccagcgtt ccgctctatc acctgccgaa
     6541 gatgcagcgt tggcttaagg aaagcggctt ttacggcgac aaggactgct tctctcacgg
     6601 ctacctggac gtggtcagga aggcgacggt gcccgccgcg taaacggtcg gggtgcaaat
     6661 gagccttgca agcggcacaa tcctgcccct ttgtgatccg cccattgacg tgggcgtcgg
     6721 catcgggccg gcagggttga ggtgagcttc aagatgcagc aattcgacat ggtcatcgtg
     6781 ggagcaggtg tatccggcat cggagccgcc tgccactttc gggagaaatg tccggggaag
     6841 acgtttgcca ttctcgagtc gcgcgatgcc attgggggta cctgggacct gttccgctat
     6901 ccgggaatcc gctccgacag cgacatgcac acgttcgggt acaacttcaa accctggcgc
     6961 aatccgaagg ccatcgccga cggcccgtcc atcagggagt acgtgcggga agcggcgcgg
     7021 gagcacgacg tcgatcctca catccgcttc aaccaccgcc tgcgctccgc ccgctggagc
     7081 tcggactcgg ctacctggac gctcgaggtg gaaacccccg atggcgtggt ggagttctcc
     7141 tgcaactttc tcttcatgtg cggcggctac tacagctacg cgggcggcta cacgcccgac
     7201 ttcccgggca tggccgattt cgagggtccc gtcgttcacc cccagcagtg gccggatgag
     7261 ctcgactatt cgagcaagcg cgtgctgata atcggcagcg gggccacggc ggtgacgctg
     7321 ctgccggcga tcgccgagcg ggcagcgcac gtcaccatgc tgcagagatc gccgacctac
     7381 atggtttccc gtcccgatcg ggatgccatc gccaaccggc tccgccggat cttcccggag
     7441 aaggtggcgt atgcgatcac ccgctggaag aacacgaccc tgcagcagtt cttctatcgc
     7501 cgcaccaggg tgaatccgga gtgggtaaag gatcgtctgc tccgtggagt gcgggcacag
     7561 ctcggtccgg acttcgatgt gcagacccac ttcacgccga gttacaaccc gtgggaccag
     7621 cggctctgcc tggtgcctaa cggcgacttt ttccgcgccc tgcgccaggg caaggccacc
     7681 gtggtgaccg acctgatcga gtccttcacc tcctccgggg tcaggctgaa gtccgggcag
     7741 accatcgagg ccgacgtcat cgtcacggcg acgggcctga atctcgaggt gatgtcgggc
     7801 gtcgagttct gggttgacga ggccaaagtc aacttcccgg acacgttcac ctacaagggc
     7861 atgatgttct ccggcgtgcc gaacctggtt cagacgttcg gctacatcaa cgcctcctgg
     7921 acgttgcgcg ctgaccttac ctcagaatac gtgtgcaggc ttgtcaatcg catggacgag
     7981 ctcggtgttt cccaggtcgc tgcgcagttg cgcgagcagg atcgtgacat gccgggcaag
     8041 cagtggatcg acgacttcac gccgggatac atgcagcgct ccatgcatct gtttccgaag
     8101 cagggcgaga aagatccctg gcgcaacacc cagaattatg cccaggacaa aaaggccatc
     8161 aggcacgctc cactggagga cggggtcctt acttt
//
LOCUS       DBNHKV010000354         5906 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1962214, whole genome shotgun sequence.
ACCESSION   DBNHKV010000354 DBNHKV010000000
VERSION     DBNHKV010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1962214"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="AC16PP_11285"
     CDS             <1..1168
                     /locus_tag="AC16PP_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HIR6482255.1"
                     /translation="EQNWQTVIEGSESHYLTGLLCFKDHLVVCERIDGLDQIRIRSGE
                     EEHHVVFPESAYAVGLGTNAEYDSATLRLHYESMVTPSTVYDYDIARRKLIQRKVREI
                     PTGYDRTRYETARRTVTARDGAQVPLSIVYPRDFPRDGTAPVHLYSYGAYGHAMSPTF
                     SPSRLSLLDRGFAFAVAHVRGGDDLGRQWYEDGKLDKRTNTFNDFVDCARHLIDSGFS
                     SPGLVVISGGSAGGELMGAAINAAPTLWGAVVAHVPFVDVLSTMLDAGLPLTPIEWPE
                     WGNPIEDKAAFELIRSYSPYDQLAPGAYPPILVTAGLNDPRVTYWEAAKYVAKLRHLK
                     TDENWLLLKTNMSAGHSGESGRFDSLRELAEEYAFFLVSLEAAGWHPRGEDDQA"
     gene            complement(1159..1401)
                     /locus_tag="AC16PP_11290"
     CDS             complement(1159..1401)
                     /locus_tag="AC16PP_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482256.1"
                     /translation="MDYVGIDPAVGLECGHCKHISRLSYSGLLDLVLECERAECDGCG
                     RLMMHDWTTVTVVQNIIKKRMRQANEAKVRHLVHHA"
     gene            complement(1562..2779)
                     /locus_tag="AC16PP_11295"
     CDS             complement(1562..2779)
                     /locus_tag="AC16PP_11295"
                     /inference="COORDINATES: protein motif:HMM:NF024732.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HIR6482257.1"
                     /translation="MKELSALLEPGQRIFVAGSSNEPTGLLEVLADQTLPPEIEFLQF
                     PLAGYNRFDFTAFSEDAIFTTFFMTPHLRDADPARVRFLPMQMRAVYDFLAEGVDVVL
                     LQVARNASGQLCVGPNVDFVEAAMMGAKTVAAELNHGFVAPAGCPVVDAGRIDFLMET
                     DRSLFEMAPPEIDGAAARIGEHVAQLVRDGDCIQTGIGSIPAAILARLGDRNDLGMHG
                     GLIDDGGMALIRNGNMTGVNKPIDTGKHIAGMALGSRQLLDWLAQTPAVQFRGANHTH
                     EVNVIRELPQFLSVNSAVEVDLFGQVNAEVAGGRQISGTGGSVDFMRAAKASPGGRSI
                     VAMNATARGGKVSRIVPKVELVTALRTDVDIIVTEHGVAQLRALSAAERVRALIEIAA
                     PEFRDGLRKVASG"
     gene            2849..3697
                     /locus_tag="AC16PP_11300"
     CDS             2849..3697
                     /locus_tag="AC16PP_11300"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF044908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6482258.1"
                     /translation="MIHGNPADLVDLLSVHPTREEGYQAGQVREARSRVYGGQFLGQG
                     ICAASQIGHGALRSFHGYYLRPGATDLGIGYQVERLGDMVVGVSAVQREKQLFLMMAR
                     FGPFCPAPPEEMPDVPPPEDCIPRERGIAGLDRDTDSTWAVTDSPFDNRFVEDIWNED
                     YRRPLHHVWFRLRDPGALPGHIDQIVMQAIAAYYADDTIMDNALFPHGWHRCWSELQT
                     ASLDHAMWFHRPMDLRRWHLFAQDSPVASGGRAMTRGTIFDDRGAVVASAAQEILLRE
                     PPDDKP"
     gene            3694..>5906
                     /locus_tag="AC16PP_11305"
     CDS             3694..>5906
                     /locus_tag="AC16PP_11305"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6482259.1"
                     /translation="MTPLAQFEPGVRAWFTERFPSPTAVQAQSWPRIAAGEHVLITAP
                     TGSGKTLTAFLWALNQYASGSWVPGHTRVVYISPLKALNNDIRRNLLEPLRSLREDHE
                     FPPVSVQTRSGDTPQSERQRMLRRPPDILITTPESLSLMLTTTRGRLALGSVETVILD
                     EIHSIVDNRRGAQLMTSLERLTDLCGEIQRLALSATVRPLEGIAEYVGGYTADGAPRR
                     VSIVDPGSAKEIEFRVRYPDEVRLALENGRKIWDPLSEVFRRQIEHNASTLFFTNSRR
                     LAEKITLKINEDQAGPIAYAHHGSLAREIRTEVEERLKAGSLKAIVATSSLEMGIDIG
                     HLDEVVLVQSPPSIAATLQRIGRAGHGVGETSVGTLYPTHANDFLEAIVLAKAVAGGD
                     IEPLTPQENALDVLAQIIVSMCASESWQLDDVFALLRRSTPYHALPREQFDLLIDMLA
                     GRYAGSRVRELKPRIVLDRINNTIRAHKGAVLALYNSGGTIPDRGYYQLRHADTGAVI
                     GELDEEFVWEATTGQIFSMGTQNWQVTRVTHNDVLVRSAPPGTTAPPFWRSESYNRSF
                     HFSERIARELEQAEALLDDGRNDDLRSTFTSAGGFEPEAAVELLDYLTRQREASEAPL
                     PHRHHLLLELVRSGPGGYRGPDDTRQLVLHTFWGGRLNRPWALALEAAWQDALGRRAE
                     IHADNNAIVIQVKGEPDPARVLAMVTADNLDELLRRSLERSGFFGARFRECAGRAL"
BASE COUNT         1122 a         1950 c         1825 g         1009 t
ORIGIN      
        1 cgagcagaac tggcagacgg tcatcgaagg ctccgagagc cactacctca ccggcctgct
       61 ctgcttcaaa gatcacctgg tggtgtgcga gcggatcgat ggcctggacc agatacgtat
      121 ccgcagcggc gaagaggaac accacgtcgt gtttccggag agcgcatacg cggtcggcct
      181 gggaacgaat gcggaatacg acagcgcaac tctgcggctg cactacgaat ccatggtcac
      241 gcccagcacg gtctacgatt acgacattgc acggcgcaag ctgattcagc gcaaagtccg
      301 ggaaatccca accggctacg accggacacg atacgaaacc gcgcggcgca ccgtaaccgc
      361 tcgcgacggc gcgcaggtac ccttgagcat cgtgtacccc agagactttc cgcgcgacgg
      421 aaccgcgccc gtgcacctct actcctacgg agcgtacggc catgccatga gcccgacatt
      481 ttcgccgagc cgtctgtccc ttctggatcg gggcttcgct ttcgccgtcg cgcacgttcg
      541 cgggggcgac gacctgggac gacagtggta tgaagacggc aagcttgaca agcgaaccaa
      601 cacgttcaac gacttcgtgg attgcgctcg gcacctgatc gactccggat tctcttcacc
      661 ggggctggtc gtcatatccg gcggcagcgc cggaggcgag ctgatgggtg ctgccatcaa
      721 cgcagcgcca acgctgtggg gagccgtggt cgctcacgtg cccttcgtcg acgtgctgag
      781 cacgatgctg gatgccggcc tgccgctcac ccccatcgag tggccggagt ggggtaaccc
      841 gatcgaggac aaagcggcct tcgagctgat cagatcatac tcaccctacg accagctcgc
      901 gcccggcgcg tacccgccca tcctggttac cgcaggactc aacgacccca gagtcaccta
      961 ctgggaggcc gcaaagtacg tcgccaagct gcggcatctc aagacagacg agaactggct
     1021 cctgctgaaa accaacatgt ctgccggcca tagcggagag tccggccggt tcgactcgct
     1081 tcgcgaactg gccgaggaat atgctttctt tctggtgagc ctcgaagccg ccggatggca
     1141 tccgcgcggc gaggacgatc aggcgtgatg aaccaggtga cggacctttg cctcgttggc
     1201 ctggcgcatg cgcttcttga tgatgttctg cacaacggtg accgtcgtcc agtcgtgcat
     1261 catcagacgc ccgcacccgt cgcactcggc gcgctcgcat tctagaacaa ggtccagaag
     1321 accgctgtag gacaaacgtg agatatgctt gcagtggccg cactccaggc ccaccgcggg
     1381 atcgatgcct acgtaatcca tgacgctgta ctccctttcg tcacgcccgc cattctaaag
     1441 gacggatttc gtgattgagc ggtactttag cggctttgtg actccgtgca cactcctgct
     1501 atgacgcagt ggcgcctgac gcgccagacc aagccttgca gtcactgccg aacgacgggt
     1561 atcagccgga ggccactttc cgcaggccat cacggaattc cggtgccgcg atctcaatga
     1621 gcgcgcgtac gcgctcggcc gccgacagcg cacggagctg ggcgacgcca tgctccgtga
     1681 caattatgtc cacgtccgtg cgcaacgcgg taaccaactc gactttcggc acgatgcggg
     1741 aaaccttgcc cccccgagcg gttgcgttca tggcaacgat ggatcgtccc cccggagagg
     1801 ctttcgccgc gcgcatgaag tcgacggagc cgcccgttcc ggaaatctgc ctgccgccag
     1861 cgacctcggc attgacctgg ccgaacaggt ccacctcgac ggcggagttg acagaaagaa
     1921 actgcggcag ctcgcgtatc acgttgacct cgtgagtgtg gttggctccc cggaactgta
     1981 ccgccggggt ttgagccagc cagtcgagca gctggcgaga tccgagcgcc atgccggcta
     2041 tgtgttttcc tgtgtctatg ggcttgttaa cgccggtcat gttcccatta cggatgagcg
     2101 ccatcccgcc gtcgtcgata agcccgccgt gcatgccgag atcattcctg tcgccgagcc
     2161 ttgcaaggat agcggcaggg atgctgccta tgccggtctg aatgcagtcg ccgtcgcgaa
     2221 cgagttgcgc gacgtgctca ccgatgcgcg ccgccgcccc atctatctcc ggaggagcca
     2281 tctcaaacag cgaacggtcc gtctccatga gaaagtcgat ccgaccggcg tcgaccaccg
     2341 gacaacccgc gggggcgacg aagccgtgat tcagctcggc cgccacggtc ttcgcgccca
     2401 tcatcgcggc ctccacgaag tccacgttgg ggcctacgca gagctggccg ctggcattgc
     2461 gcgcaacctg gaggagcacg acgtcgacac cttcggccag gaagtcgtac acggctcgca
     2521 tctgcatcgg aaggaaacgc acgcgcgccg gatcggcatc gcgaagatgc ggcgtcatga
     2581 aaaaggttgt gaagatcgcg tcctcggaga aggccgtgaa gtcgaagcgg ttgtatccag
     2641 ccagcggaaa ctggaggaac tcgatttccg gcggcagagt ctgatccgcc agcacctcga
     2701 gcaacccggt cggctcgttg gaactgcccg ccacgaatat cctctgaccc ggctccagca
     2761 gagccgataa ctctttcatg atttcaccat cctggaggca agggcgagat tctagcctcg
     2821 cccaggggca attcgtaata atcggcagat gattcacggc aaccccgcag acctggtcga
     2881 tttgctttca gtccacccga cgagagagga aggctatcaa gcgggtcagg tgcgcgaagc
     2941 acgctcgcgg gtatacggag gccagtttct cggtcagggc atctgtgccg ccagccagat
     3001 cgggcacggc gccctgaggt cctttcacgg ctactatctg cgtcccgggg cgacggacct
     3061 gggcatcggc taccaggtgg agcgcctcgg cgacatggtt gtcggcgtgt ccgccgtgca
     3121 gcgcgagaag cagctgtttc tcatgatggc gcgcttcggc cctttctgcc ccgctccacc
     3181 cgaggagatg cccgacgtgc cgccccccga ggactgcatt ccacgggagc gcggcattgc
     3241 cggcctggac cgagacaccg attccacatg ggccgtaaca gactcccctt tcgacaaccg
     3301 gttcgtcgag gacatctgga acgaagacta ccggcgaccg cttcaccacg tctggttccg
     3361 cctgcgcgat cccggcgcgc tgcccggcca tatcgatcag atcgtgatgc aggcgattgc
     3421 cgcctactac gcggacgaca ccatcatgga caacgcgctc tttccacacg gctggcaccg
     3481 atgctggagc gaactccaga cggcgagcct cgaccacgcc atgtggttcc accgacccat
     3541 ggatctcaga cgctggcacc tctttgccca ggacagcccg gtggcaagcg gcgggcgcgc
     3601 catgacccgt gggaccatat tcgacgatcg gggcgcggtg gtggcctcag ccgcccagga
     3661 gatccttctg cgcgaaccac cggacgacaa gccgtgacac ctctggcgca gttcgaaccc
     3721 ggagtacgtg cgtggttcac cgagcgcttt ccttccccca ctgccgttca ggcgcagagc
     3781 tggccgcgga tcgcagcagg ggagcatgtg ctgatcacgg caccgacggg cagcggcaaa
     3841 acgctgaccg cttttctgtg ggcgttgaac cagtacgcgt ccggatcgtg ggtgccgggc
     3901 catacccggg ttgtctacat ctcgccgctg aaagcgctga acaacgacat ccggcgcaac
     3961 ctgctggagc ccctgcgatc gcttcgggag gatcacgaat ttcccccggt aagcgtgcag
     4021 acccgatccg gcgacacgcc gcaaagcgaa agacaacgaa tgctgcggcg gccgccggac
     4081 attctgatca cgacgcctga aagcctgtcc ctgatgctga ccaccactcg gggacgactg
     4141 gcgcttggca gcgtggagac ggtgatactc gacgagatac attccatcgt ggacaaccgg
     4201 cgcggcgcgc agctgatgac ctcgctggaa agactgacgg atctgtgcgg cgagattcag
     4261 cggctcgcgc tgtctgctac cgtccgccca ctcgaaggga tcgcggagta cgtgggcggc
     4321 tacacggccg acggcgctcc gcgcagggtc agcatcgtcg atccaggctc cgccaaggaa
     4381 atcgagttcc gcgtgcgcta ccccgacgaa gtcaggctcg ccctggagaa cggcaggaag
     4441 atctgggatc cgttgtcaga ggtttttcgg cgccagatcg agcacaacgc ctcgactctg
     4501 tttttcacca acagccggcg cctggccgaa aagatcacct tgaagataaa cgaggaccag
     4561 gccgggccca tcgcctacgc tcaccacggc tcgctggcca gagagatccg cacggaggtg
     4621 gaagagcggc tgaaggccgg cagcctcaag gccatcgtcg cgaccagctc tctggagatg
     4681 ggtatcgaca tcggccatct cgacgaggtc gtcctggtcc agtctccgcc ttccatcgcg
     4741 gccacgcttc agcgcatcgg tcgggccggt cacggcgtgg gagagaccag cgtcggcacg
     4801 ctctacccta cccacgcaaa cgactttctc gaggccatcg tcctggccaa ggcggtagcc
     4861 gggggcgaca tcgagccgct gactcctcag gagaacgcgc tggacgtgct cgcccagatc
     4921 atcgtgtcca tgtgcgccag cgaaagctgg cagctcgacg atgtcttcgc tctgcttcgc
     4981 cgcagcaccc cgtaccatgc gctgccgcgg gagcagttcg atctgctgat cgacatgctg
     5041 gccgggcgct atgcaggctc acgggtgcgg gagctgaaac cgcgcatcgt tctggaccgg
     5101 ataaacaaca cgattcgagc gcataagggc gcggtcctcg ccctgtacaa ctccggcggc
     5161 accatccccg accgcggcta ttaccagctt cgccacgccg acaccggtgc ggtgatcggc
     5221 gagctcgacg aggagttcgt ctgggaggcc accacgggtc agatcttcag catgggcacc
     5281 cagaactggc aggtcacgcg ggtgacgcac aacgatgtgc tcgttcgctc ggcaccaccg
     5341 ggcacgacgg ctccgccgtt ctggcgctcg gagtcgtaca accggagctt ccacttctcg
     5401 gagcgcatcg cccgcgagct cgaacaagcc gaagccttgc tcgacgacgg gcgcaacgac
     5461 gatcttcgat ccacgttcac cagcgcgggc ggcttcgagc ccgaggcggc cgtcgagctg
     5521 ctcgattacc tgacccggca gcgggaggcg agcgaagccc ccctgcccca tcggcatcat
     5581 ctgctgctgg aactcgtccg cagcggaccg ggcggttatc gcggaccgga cgatacccgc
     5641 cagctggtgc tgcatacgtt ctggggcggc cggctgaacc ggccgtgggc gctggcattg
     5701 gaggccgcct ggcaggacgc gctcggcagg cgcgcggaaa ttcacgccga caacaacgcc
     5761 atcgtcattc aggtaaaggg cgaacccgat cccgcgcggg tgctcgccat ggtgacggcc
     5821 gacaacctcg acgagctgct gcgcaggtcc ctggagcggt ccggattctt cggcgcgcgt
     5881 tttcgagagt gcgccggccg ggccct
//
LOCUS       DBNHKV010000355        10673 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_262636, whole genome shotgun sequence.
ACCESSION   DBNHKV010000355 DBNHKV010000000
VERSION     DBNHKV010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10673)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10673)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10673
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_262636"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="AC16PP_11310"
     CDS             <1..349
                     /locus_tag="AC16PP_11310"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6482260.1"
                     /translation="TLRQDGQPVSSVVAYARDGDTLVVSTPGTTFKARSAGRDGRATL
                     CVITNQEPFNFVSVEGRVDVETDELVRNTRLVFENIEGTGFVEPEDLEGWLAAEQRVI
                     LRIHPQRVFGVIR"
     gene            446..2614
                     /gene="scpA"
                     /locus_tag="AC16PP_11315"
     CDS             446..2614
                     /gene="scpA"
                     /locus_tag="AC16PP_11315"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HIR6482261.1"
                     /translation="MAENPTRKSDPAGQDEATWKELAAKELRGKPLDELTWVSPEGIP
                     IKPLYTEADLEEIEHLGSFPGLEPYVRGPRATMYTNRPWTIRQYAGFSTAEESNAFYR
                     QNLAAGQKGLSVAFDLATHRGFDSDHPRVVGDVGMAGVAIDSVEDMKILFDGIPLDQM
                     SVSMTMNGAVLPVMAGYIVAAEEQGVAPKDLAGTIQNDILKEFMVRNTFIYPPEPSMR
                     IVADIIGYTAEHMPRFNSISISGYHMQEAGANAVQEVAFTLADGIEYVRAALATGQEI
                     DAFAPRLSFFFCIGMNFFMEIAKLRAARVLWHELMSQFKPQNPTSLMLRTHCQTSGVS
                     LQEHDPYNNVVRTTIEAMAAVLGGTQSLHTNAFDEAIALPTPFSARIARNTQIIIAEE
                     SQMTRTVDPLGGSYYVESLTNSIVEAAREIIDEVEAMGGMTKAVVAGMPKQRIEEASA
                     RRQARVERGEDVIVGVNKYKLEKDDSVFDVLAVDNSKVRDAQLKRLARIRASRDEAAT
                     QAALDALTEAARTGEGNLLDLSVAATRLRATVGEVSTALEKVWNRHTPVARMTPGVYA
                     DAHQEDEQFVRVRDEVKVFTELEGRPPSILVVKMGQDGHDRGAKVISNAFGDLGFEVK
                     MGPLFQTPAEAASQATDEAVNVVGVSTLAGGHKTLVPEMIEALKAAGNEDVIVVVGGV
                     IPQKDYQFLYDCGVHAIYGPGTNIPEAASDVLQLIPGKNR"
     gene            2643..3053
                     /gene="mce"
                     /locus_tag="AC16PP_11320"
     CDS             2643..3053
                     /gene="mce"
                     /locus_tag="AC16PP_11320"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HIR6482262.1"
                     /translation="MIGKLNHVAIVVPDLESAAALYRDTLGATVSDPVPLPDHGVTTV
                     FVELPNSKIELLHPLGDGSPVANYLERNPKGGMHHLCYEVQDIEAAIAHLVAEGATVL
                     GNGKPSTGAHGLPVVFLHPKDFCGTLIELEEVAA"
     gene            3106..3855
                     /locus_tag="AC16PP_11325"
     CDS             3106..3855
                     /locus_tag="AC16PP_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6482263.1"
                     /translation="MTTLVRIDSFDFPPGRKITARYQVVSQLGAGWESEVYLIRETAT
                     GIERTAKVFFPHRNRGNKVARRTAEKLHKLRDCPALIQYVAQETITVRRQAVTVLISE
                     YVEGDLLSEFIARQPGGRMHRFEALHLLRALALALEPIHERREYHGDLHAGNVIVRRV
                     GLDFDVRLIDVYHLDQRKSLSVKDDVCDAIRIFYDALGGQRFYARQSPEIKAICRGLK
                     RSLISERFRTAGQLRRYLDTMSWESEPRRGR"
     gene            3864..5105
                     /locus_tag="AC16PP_11330"
     CDS             3864..5105
                     /locus_tag="AC16PP_11330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6482264.1"
                     /translation="MRGTIARLSALLTAVAILLIGHGLQLTLLPVHAEGLGWSASAIG
                     TSGSAYFLGFVVGCVLIPPVVGRVGHIRTFMVMGALATVALLAAGLLVTLPAWLIFRF
                     VTGFALSGLYMVIESWLVDVTPSNNRGTILALYTVICLLGMSVGQAFLGIASPMSLEL
                     FMAGAALICLAIVPVGLARVAAPHPIPSARFSPAVLLKASRVAMVCAFAGGLVTGAFW
                     TLGPVLARAFGLDAAQVGGMMGAGIIGGAAVQIPVGRLSDRTDRRLVIGGLMAAGALV
                     ALCGWFLAGEDSIALSVIMFLFGAATFCIYAVCIVHASDNADIPLVEIASGILIMNSA
                     GSIAGPIVVALLMDRFGPESFFAYVLACLAPVAVWVFYRTAVVERSREHEHASILPRT
                     TQAIAELSPSEPDVGTDTANN"
     gene            complement(5121..6482)
                     /locus_tag="AC16PP_11335"
     CDS             complement(5121..6482)
                     /locus_tag="AC16PP_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR6482265.1"
                     /translation="MLKRSGQTFPALNVYLTRLVEGRLWLQVIIGMVLGILTGVAIGP
                     AAGLLDMETGVVVGNWLAFPGRLFLVTIQMVVVPLVFASIVRGLTGGPDMAELKRVGI
                     WVSGFFLVTTALAATIGIQITLLLEPGNHLDLGTQLMDQLSAADPGYVPTAPMLQNLP
                     DIVIDMLPGNPLNALVEGQMLQIVLFAFIIGLALISLPEATSRPLADLMASVQEVCMA
                     VVRWAMTIAPFAVFGLMAQLTSRTGLQTLIGLTAYVGTVLLGLLALLAVQLAFVRLFS
                     PYGPCRFLSAARELLLLAFSTSSSAAVMPLSMKTAMEKFRVRASVAQLVIPLGATINM
                     NGTALYQAVAAVFLAQVFGVEMTGAGLALIVAMAIGASIGSPATPGVGIVILASILAG
                     IGVPPAGIALIIGVDRILDMCRTAVNVSGDIVACIFADRWVLGERARDGAESPSPPEE
                     AVT"
     gene            6612..7472
                     /locus_tag="AC16PP_11340"
     CDS             6612..7472
                     /locus_tag="AC16PP_11340"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6482266.1"
                     /translation="MQRIIDLLNVQLFTLGEQPVTVMQLLVVPLLIVVGILLIRWGAR
                     VISRRMMAREASPDVVHLVRRVFYIVAIAILVITALDLVNVPLTAFAFVSGAIAIGVG
                     FGAQNIINNFISGWILMWERPIRIGDFLEIGETRGTVEAINTRSTRIRRIDGVHLLVP
                     NSHLLENTVVNWTLVDRLARTTVRVGVAYGSPTAQVRELISQAVIEQDGVLEDPKPNV
                     VFEDFGDNSLIFDAYFWANAGGDRDLRTIRSDIRFRIDELFREHDIVIAFPQRDIHVD
                     GSLVLTRKET"
     gene            complement(7480..8442)
                     /locus_tag="AC16PP_11345"
     CDS             complement(7480..8442)
                     /locus_tag="AC16PP_11345"
                     /inference="COORDINATES: protein motif:HMM:NF013694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HIR6482267.1"
                     /translation="MQTDEDGLVFAMALDGNGGADPVGLSQAGDARPTWLHFDYSSSA
                     TEQSLIHIGLSASITESLTRPDARPRTVAMEGGVLVLLRGVNTNPGANPEDMVSVRIW
                     LEPNRMISVRQRRLLSVQDLKAELEQGRGPRSVPELAVELIERLADRIAIFVDGIEDR
                     AALCEADADEASPTELRATVAELRRQTAAVRRFLAPLRDALESLFRNSKGILDEDRSF
                     RIREQSDRITRYVEDLDLVRERAMVIHEELLNRVAQEQNARVYLLSIVAAIFLPITFI
                     TGLFGMNVAGLPGVDDPRSFWLVAGIMTAVSGGVIVWLRRRRWL"
     gene            8607..9023
                     /locus_tag="AC16PP_11350"
     CDS             8607..9023
                     /locus_tag="AC16PP_11350"
                     /inference="COORDINATES: protein motif:HMM:NF017434.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease family protein"
                     /protein_id="HIR6482268.1"
                     /translation="MGWREWVSLPGLRIDRVKAKIDTGARTSALHAFELDTVNRRDGL
                     WVTFAVHPNQRDEESVVRSQAKVTDRRIVRDSGGHEEERFVITTDVEMGGERWPIELT
                     LTARDNMGFRMLLGRTAMRGRYMVDPARSYTTGRRM"
     gene            9020..9925
                     /gene="rimK"
                     /locus_tag="AC16PP_11355"
     CDS             9020..9925
                     /gene="rimK"
                     /locus_tag="AC16PP_11355"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HIR6482269.1"
                     /translation="MKIGILSRNRKLYSTRRLVEAAKARGHEAVVVDTLRCYMNMATR
                     RPSIHYQGKELTGLDAVIPRIGASISFYGAAVVRQFEMMGVYAVNESVAITRARDKLR
                     SMQLLARRDIGMPVTGFAHSPDDIPDLIDMVGGAPLIVKLLEGTQGIGVVLCDNRKSA
                     ESVIEAFLGINVNIMVQEFVQESAGSDIRCFVVGDKVVAAMSRHAQPGEFRSNLHRGG
                     SAKATKITPTERRTAVSAARAMGLNVAGVDLIRSNHGPLVLEVNSSPGLEGIESATGM
                     DVAGRIVEFIEQNGRPGRTRTRGRG"
     gene            9918..>10673
                     /locus_tag="AC16PP_11360"
     CDS             9918..>10673
                     /locus_tag="AC16PP_11360"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HIR6482270.1"
                     /translation="MGKHLNAAFKIAGHTIWPGERVRFDLPGAQLYTHTPLDMPVEVV
                     NGRRAGPTLLICAAIHGDEINGVEIIRRLSALKALSRLHGTLILVPVVNLFGFIHQSR
                     YLPDRRDLNRCFPGSGGGSLGSRVAHLFFEEVVVRSTHIVDLHTGAIHRDNLPQIRAT
                     LDDPVVREMALGFSIPVIVNAGLIDKSLRSEAGKRGIPVITYEGGEALRFDESCIVAG
                     VRGVTNVMRTLGMLPAKHIRRVRAEPYVSRSTVW"
BASE COUNT         1917 a         3339 c         3525 g         1892 t
ORIGIN      
        1 gacgctgcgc caggatggcc agccggtcag ctcggtggtg gcttacgcgc gcgacggcga
       61 cacgctcgtg gtcagcacgc cgggcacgac gttcaaggcg cgttcggccg gccgcgacgg
      121 gcgggccacg ctctgcgtca ttaccaatca ggagccgttc aatttcgtct cggtggaggg
      181 gcgggtcgac gtggagaccg acgagcttgt tcgcaacacg cggctggtgt tcgagaacat
      241 cgagggcacc ggcttcgtcg agccggaaga tctggagggc tggcttgcgg ccgagcagcg
      301 ggtgattctg cggatacacc cccaacgcgt gttcggggtt attcgctgac gggggtcatc
      361 cgccggtccc cgcggggctg gacgttgcta taatcgccgg ccgtttgcgc gctgggcgcg
      421 actcctgagg ctcgaggtag gaaccatggc agagaacccg acacgcaaga gcgaccctgc
      481 gggacaggac gaggcgacct ggaaagagct ggctgcgaag gaactgcgcg gcaaaccgct
      541 cgacgagctc acctgggtga gcccggaggg catcccgatc aagccgctgt acaccgaagc
      601 ggatctcgag gaaatcgagc atctgggctc attccccggt ctcgagccct acgttcgtgg
      661 gccgcgcgcg accatgtaca ccaatcgtcc gtggactatc cgtcagtacg cgggcttctc
      721 cacggcggag gaatccaacg ccttttaccg ccagaacctg gctgcggggc agaagggcct
      781 gtcggtggcc ttcgatctgg ccacccaccg ggggttcgac tcggaccacc cgcgcgtcgt
      841 gggcgacgtc ggcatggcgg gggtcgccat cgactccgtg gaggacatga agattctctt
      901 cgacggaatt ccgctggatc agatgtcggt gtccatgacc atgaacggcg ccgttctgcc
      961 ggtgatggcc ggctacatcg tcgccgctga ggagcagggg gtggcgccca aggatctcgc
     1021 ggggaccatc cagaacgaca tcctcaagga gttcatggtc cgcaacacgt tcatctaccc
     1081 gcccgagccg agcatgcgga tcgtggcgga tatcatcggc tacaccgccg agcacatgcc
     1141 gcgcttcaat tccatctcca tctccggcta tcacatgcag gaggcggggg ccaacgcggt
     1201 tcaggaagtg gccttcacgc tggccgacgg gatcgagtac gtccgtgccg ccctggcgac
     1261 ggggcaggag atcgacgcgt tcgcgccgcg tctgtcgttc ttcttctgca tcgggatgaa
     1321 cttcttcatg gagatcgcca agctgcgggc ggcgcgcgtt ctctggcacg agcttatgag
     1381 tcagttcaag ccgcagaacc ctacctcgct gatgctgcgt acccactgcc agacctcggg
     1441 ggtgtcgctg caggagcacg atccctacaa caacgtggtc aggacgacga tcgaggccat
     1501 ggcggccgtg ctcgggggta cccagagcct gcataccaac gcgttcgacg aggccatcgc
     1561 gctgcctacg ccgttctccg cgcgcatcgc ccgcaacacc cagatcatca tcgccgaaga
     1621 gtcccagatg acccgcacgg tcgaccccct gggcggcagc tattacgtgg aaagcctgac
     1681 caactccatc gtggaggcgg cccgtgagat catcgacgag gtcgaggcca tgggcggcat
     1741 gaccaaggcg gtcgtggccg gcatgcccaa gcagcgcatc gaggaggcct cggcgcgccg
     1801 ccaggcccgg gtcgagcggg gcgaggacgt aatcgtgggg gtgaacaagt acaagctcga
     1861 aaaggatgac tccgtcttcg acgttctcgc ggtggacaac agcaaggtgc gcgacgcgca
     1921 gctcaagcgg ctggcgcgca ttcgcgcctc ccgggacgag gccgccactc aggccgccct
     1981 ggacgcgctc accgaggccg ccaggacggg cgagggcaac ctgctggatc tcagcgtggc
     2041 agcgacgcgc ctgcgggcaa ccgtcggcga ggtctcaacc gcgctggaga aggtctggaa
     2101 tcgccatacg cccgtagcgc gaatgacccc gggggtgtat gccgacgccc atcaggagga
     2161 cgagcagttc gtgcgggttc gcgacgaggt caaggtgttc accgagctcg aagggcggcc
     2221 gccgagcatc ctggtggtca agatgggcca ggacggccac gaccgcgggg ccaaggtcat
     2281 ctccaatgct ttcggcgacc ttggcttcga agtgaagatg gggccgctgt tccagacccc
     2341 ggcggaggcc gcgtctcagg cgaccgacga ggcggtgaac gtcgtaggcg tctcgaccct
     2401 ggctggagga cacaagacgc tggtgcccga gatgatcgaa gcgctcaaag cggcgggtaa
     2461 cgaggacgtg atcgtcgtgg tgggcggggt gattccgcag aaggactatc agtttctcta
     2521 cgactgcggc gtgcacgcca tctacgggcc gggcaccaac ataccggagg cggccagcga
     2581 cgtgctgcag ctgattccgg gcaagaaccg atgaacaacc tgaacacgga acggaagagc
     2641 aaatgatcgg taagctgaac cacgttgcca tcgtcgtccc ggacctcgaa tccgcggcgg
     2701 cactctacag ggacacgctg ggcgccacgg tgtccgatcc cgtgccgctc ccggatcacg
     2761 gcgtcacgac ggtcttcgtc gagctgccca acagcaagat cgagctgcta catccgctgg
     2821 gtgacggatc tccggtggcg aactacctcg agcggaaccc gaagggcggg atgcatcacc
     2881 tgtgctacga ggtccaggac atcgaagccg ccatcgcgca cctcgtcgcg gagggcgcca
     2941 cggtgcttgg gaacggcaag ccgtcgacgg gggctcatgg cctgcccgtg gtctttctgc
     3001 accccaagga tttctgcggc acgctgatcg agctcgaaga agttgctgcc tgaacgtgcc
     3061 cggatcgtag ccagcccctg agcgcccgag aggcttgatg cagcggtgac cacccttgtc
     3121 cgcatcgaca gcttcgattt tccgccgggc cgcaagatca cggcccgata tcaggtcgtc
     3181 agccagcttg gagccggctg ggaaagtgag gtctatctca tccgcgagac ggccaccggt
     3241 atcgagcgga cggccaaagt gttctttccg caccgaaacc ggggcaataa ggtggcgcgc
     3301 cgcacggcgg aaaagctaca caagctcagg gactgcccgg cgctgatcca gtacgtggcc
     3361 caggaaacga tcaccgtcag gcggcaggcc gtgacggtgc tcatctcgga gtacgtggag
     3421 ggcgatctgc tcagcgagtt catcgcgcgg cagccgggtg ggcggatgca ccgcttcgag
     3481 gcgctgcacc tgctgcgggc cctggcgctg gcgctcgaac cgattcacga gcgtcgcgag
     3541 taccacggcg atctgcacgc gggcaatgtc atcgtgcgtc gggtcggtct cgatttcgat
     3601 gtccggctca tcgacgtcta ccacctcgat cagcgaaagt cgttgagcgt aaaggacgac
     3661 gtctgcgatg ccatccggat cttttatgat gccctgggcg gccagcgttt ctacgcccgg
     3721 caatcgcctg agatcaaggc gatctgtcgc ggtctgaagc gttcgctcat ctcggagcgc
     3781 tttcgcaccg ctggtcagct gcgccgttac ctggacacca tgtcctggga atccgagccc
     3841 cggcgagggc ggtaggcaga accatgcgcg gaacgatcgc ccgtctgagc gctctgctga
     3901 ccgctgtcgc catcctgctg atcggccacg gtctccagct tacgctcctg ccggtgcacg
     3961 cagagggtct gggttggtcg gcttccgcca tcggtaccag cgggtcggcc tattttctgg
     4021 gtttcgtggt gggctgcgtt ctgattccac cggtggtcgg cagggtcggc cacattcgca
     4081 ctttcatggt gatgggggcc ttggccaccg ttgcgctgct ggctgccggg ttgctcgtga
     4141 ccctgcctgc ctggctcatc ttccggttcg ttaccgggtt cgcgctgtcc ggtctctaca
     4201 tggtgatcga aagctggctg gtggacgtga cgccatcgaa caatcgggga acgatactgg
     4261 ctctctacac ggtcatctgc ctgctgggga tgtccgtggg gcaggctttc ctcggcatag
     4321 cctcacccat gagcctggag ctcttcatgg caggcgctgc cctcatctgc ctggcgatcg
     4381 tgcccgtggg tctggcgcgg gtggccgcac cgcaccccat tccctcggct cgcttctccc
     4441 ccgccgtcct gctgaaagca tcccgggtcg ccatggtctg cgcgtttgca ggcggtctgg
     4501 tgacaggcgc tttctggacg ctcggaccgg ttttggcgcg agccttcggc ctggacgccg
     4561 cgcaggtcgg cgggatgatg ggtgcgggca tcattggagg tgccgcggtt cagatacccg
     4621 tcggccggct ctcggatcgt accgaccggc gactggtgat cggcgggctc atggcggccg
     4681 gcgctctggt ggcgctgtgc ggctggttcc ttgctggtga agactccatc gcgctgtcgg
     4741 tcatcatgtt tctctttggt gcggcgacgt tctgcatata cgcggtctgc atcgtgcacg
     4801 ccagtgacaa tgcggacatt ccgctggtcg agatagcaag cggtattctc atcatgaaca
     4861 gcgctggttc catcgcgggc cccatcgtcg tagcgctgct gatggatcgc tttggtccgg
     4921 agagcttctt cgcctacgtg ctagcctgtc tggctccggt cgccgtctgg gtcttctatc
     4981 gtaccgccgt ggtggagcgt tcgcgggagc acgagcatgc cagcattctc cctcgcacga
     5041 cgcaggccat cgcggagctc tcgcccagcg agcccgacgt cggcaccgac actgccaata
     5101 attgagagcg gctcggggat ctaggtgacg gcctcttcgg gcgggctcgg gctctccgcg
     5161 ccgtccctcg ctcgctctcc cagcacccag cgatcggcga atatgcaggc aacgatgtcg
     5221 ccgctgacgt tcaccgccgt gcggcacatg tcgagaatcc tgtccacgcc gatgatcagc
     5281 gcgatccccg cgggaggcac gccgatgccg gccaggatgc ttgccaggat gacgatgccg
     5341 acgcccggcg tggcggggga tccgatggat gccccgatgg ccatggcgac gatcagggcc
     5401 agacccgcgc cggtcatttc caccccgaag acctgggcaa gaaagacggc ggccactgcc
     5461 tgatacagcg cggtgccgtt catgttgatc gtcgcgccga ggggaatcac cagctgcgcc
     5521 acgctcgcgc gcacccggaa tttttccatg gccgtcttca tggagagcgg catcacggcg
     5581 gccgagctcg aggtgctgaa ggcgaggagc agcaattccc tcgccgcgga gagaaaccgg
     5641 caggggccat agggggagaa caagcgcacg aaagccagct gcaccgccag caacgccagc
     5701 agacccagga gcacggtgcc gacgtaggcg gtcagcccga tcagcgtctg cagcccggtt
     5761 cggctcgtca gctgagccat cagaccgaac acggcgaacg gggcgatggt catcgcccag
     5821 cgcacgacgg ccatgcacac ttcctggacg gaagccatga gatcggccag aggtcgggac
     5881 gtcgcctctg gcagcgagat aagcgcgagc ccgatgatga aggcgaacag cacgatctgc
     5941 agcatctggc cctcgaccag cgcgttgagc gggttgccgg gaagcatgtc gatgacgatg
     6001 tccggcaggt tctgcagcat gggcgccgtc ggcacgtacc cggggtcggc ggccgagagc
     6061 tggtccatca gctgagtgcc cagatcaagg tggttgcccg gctcgaggag cagggtgatc
     6121 tgaatgccta tggtggcggc cagggccgtc gtgacgagga agaagccgct cacccagatg
     6181 ccgacccgtt tcaactcggc catgtcgggc ccgccggtca ggccgcgcac gatggaggcg
     6241 aagacgaggg gtaccacgac catctgtatg gtcaccagga acagccggcc cgggaaggcc
     6301 agccagtttc ccaccaccac accggtctcc atgtccagaa gcccggccgc cgggccgatg
     6361 gccacgccgg tcaggattcc cagcaccatg ccgatgatga cctgcagcca cagcctgcct
     6421 tccaccaggc gggtcaggta aacgttcaac gccgggaagg tctgtcccga ccgcttgagc
     6481 atgaagttcg ccaattccgt ccagctcccg cgattccggc ggttaagaaa cattcgggtg
     6541 agcggcccgt ctatacgggt ttaccgatgc tctgcagtac aatatcagct gtttgcggcc
     6601 agggtccgga cgtgcagcga attatcgacc tgctcaacgt gcagctcttt actctcggtg
     6661 agcagccggt gaccgtcatg cagctgctgg tggttccgct gctgatcgtg gtcggcattc
     6721 tgctgatccg ctggggggcc cgcgtcatct cgcggcgcat gatggcgcgg gaggccagcc
     6781 ccgacgtcgt ccacctcgtc cggcgcgtct tctacatcgt agcgatcgcc attctggtca
     6841 tcaccgccct cgatctcgtc aacgttccgc tgacggcgtt cgccttcgtc tcgggcgcca
     6901 tcgccatcgg ggtgggtttc ggggctcaga acatcattaa caacttcatc agcggctgga
     6961 tcctgatgtg ggaaaggccc atcagaatcg gggattttct ggagataggc gaaacccggg
     7021 ggaccgtgga ggcgatcaac acgcgctcca cgcgaatccg gcgtatagac ggcgtgcatc
     7081 tcctcgtgcc taacagccac ctgctggaga acacggtggt gaactggacc ctggttgacc
     7141 ggcttgcccg cacgaccgtg cgggtggggg tcgcctacgg gtcgccgacg gcccaggtcc
     7201 gcgagctcat cagccaggcg gtcatcgagc aggacggggt gcttgaagac ccgaagccca
     7261 acgtcgtttt cgaggatttt ggcgacaact ccctgatttt cgacgcctac ttctgggcca
     7321 acgcgggcgg ggacagggac ctgcgaacca tccgcagcga cattcgtttc cgcatcgacg
     7381 agctgttccg ggaacacgac atcgtcatcg cgtttccgca gagggacatt cacgtggacg
     7441 gctccctcgt gctcacccgc aaagaaacgt agagcggccc tacagccagc ggcgtcggcg
     7501 taaccagaca atgacgccgc ccgatacggc cgtcatgatg ccggcgacca gccagaacga
     7561 gcgggggtcg tcgacgcctg gcagtcctgc cacgttcatg ccgaacagac cggtgatgaa
     7621 ggtgatgggc agaaaaatcg cggccacgat ggaaagcaga tagaccctgg cattctgttc
     7681 ctgagcgacg cggttgagca gttcctcgtg aattaccatg gcgcgttcgc ggacgagatc
     7741 gagatcctcg acataacgcg ttatccgatc ggattgctcc cggatccgga acgaccggtc
     7801 ttcgtccagg atgcccttgg agttgcggaa cagggattcc aacgcgtcgc gcaggggtgc
     7861 cagaaagcgc ctgactgcgg ccgtctgccg gcgcagttct gcgacggtgg cccgcagttc
     7921 cgtggggctg gcttcgtcgg cgtccgcctc gcagagggcg gcccgatcct cgatgccgtc
     7981 gacgaagatc gcgatgcggt ccgccaggcg ctcgatcagc tccactgcca gctccggaac
     8041 cgaccgggga ccgcggccct gctcgagctc cgccttgaga tcctgaacag aaagcagacg
     8101 gcgctgccga acggaaatca tccggttggg ctccagccag atccgcacgg agaccatgtc
     8161 ttcagggttt gcgccgggat tcgtgtttac gccgcgcagc agcacgagca caccgccctc
     8221 catggccacg gtgcggggtc gggcatccgg cctggtcagg ctttcggtga tggaggcgct
     8281 cagcccgatg tggatgaggc tctgctcggt ggcgctggac gaatagtcga agtgaagcca
     8341 ggtgggccgc gcatcaccgg cctgtgacag cccgacggga tcggcgccgc cgttaccatc
     8401 cagggccata gcgaaaacca gtccgtcctc gtccgtctgc atcatgtcct taatgaaggc
     8461 aagtgtcgct aaagggagaa gactagggta aaaggctcgt cagaactact gcccgaaggc
     8521 aatgccttgt ggtgcgatcg tgagaaagcg cggcttccgc gtgacggggg ttcttcatca
     8581 ccaagaacat cctcagcagg gagatcatcg gctggcgcga gtgggtatcg ctgccagggc
     8641 ttcggatcga tcgcgtcaag gccaagatcg acacgggcgc cagaacctca gcgcttcacg
     8701 ccttcgaatt ggacaccgtg aatcgccgtg acggtctctg ggtcactttc gccgtgcacc
     8761 ccaaccagcg cgatgaggaa tctgtggtcc ggtcacaggc caaggtgacc gaccggcgta
     8821 tagtgcgtga ctcgggcggg catgaggaag agcgtttcgt cattaccacc gacgtggaga
     8881 tgggtggcga aagatggccc atcgagctca ccctgaccgc gagagacaac atggggtttc
     8941 gcatgctgct gggccgtacg gccatgcgag gacgttacat ggtcgatccg gcgcgctcgt
     9001 acacaacggg aaggcgaatg tgaaaatcgg aattctctcc agaaacagga agctgtactc
     9061 gacgcggcgg ctcgtagaag cggcgaaggc tcgcggccac gaggccgtcg ttgtagacac
     9121 gctgcggtgc tacatgaaca tggccacgcg acgtccttcc attcattacc agggtaagga
     9181 gttgaccgga ctcgatgcgg tgattcccag gatcggtgcg tccatatcat tttatggggc
     9241 cgccgtcgtc cgccagttcg agatgatggg cgtctacgcg gtcaacgagt ccgtggccat
     9301 cacccgggcc cgcgacaagc tgcgttccat gcagctgctg gcgcgaagag acatcggtat
     9361 gccggtgacg ggattcgccc actcgccgga cgacattccg gacctcatcg acatggttgg
     9421 tggcgcgccg ctgatcgtca agctgctgga aggcacccag ggcatcggcg tggttctctg
     9481 cgacaatcga aagtccgcgg agagcgtcat cgaagctttc ctcggcatca acgtgaacat
     9541 catggtgcag gagttcgtcc aggagtccgc cgggtcggac attcgctgct tcgtggttgg
     9601 cgacaaggtg gtggccgcca tgagccggca cgcgcagcca ggcgagttcc gctctaacct
     9661 gcaccggggc gggtcggcta aagccaccaa gatcacacct accgagcggc gcacggcggt
     9721 gtcggcggcg cgcgccatgg ggctcaacgt cgcgggcgtg gatctgattc gctccaacca
     9781 tgggccgctg gtgctggaag tgaacagctc gcccggcctc gaaggcatag agagcgccac
     9841 ggggatggac gtcgccggcc gaatcgtgga gttcatcgag cagaacggca ggccgggcag
     9901 gaccagaacg cgtgggcgtg ggtaagcacc tcaacgcggc gttcaagatt gccggtcaca
     9961 ccatctggcc gggcgagcgc gtgcgcttcg atcttccggg cgctcagctt tacacccaca
    10021 ccccgctgga catgcccgtg gaggtcgtca acggcaggcg cgcgggcccg acgcttctga
    10081 tctgcgctgc catccacggg gacgagatca acggcgtgga gatcatccgg cggctttcgg
    10141 cgctcaaggc attgagccgg ctgcacggaa cgctgattct ggtcccggtg gtgaatctct
    10201 tcggtttcat tcaccagtcc cgctacctcc ccgaccggcg cgatctcaat cgctgcttcc
    10261 cggggagtgg tggcggctcg ctaggctcca gagtggcgca tctctttttc gaagaggtgg
    10321 tcgtccgcag cacccacatc gtcgaccttc acaccggcgc catccaccgc gacaacctgc
    10381 cgcagatcag ggcgacgctg gatgatccgg tggttcggga aatggcgttg ggcttttcca
    10441 tacccgtgat cgtcaatgcc ggcctcatcg acaaaagcct gcgcagcgag gcgggcaagc
    10501 gtggaattcc cgtcattacc tatgagggag gcgaggcgct tcgtttcgat gagagctgca
    10561 tcgttgcagg tgtgcggggt gtgaccaacg tcatgcgcac cctgggcatg ctgccggcaa
    10621 aacacattcg acgggtgcgg gccgagcctt acgtatcccg ttccacggta tgg
//
LOCUS       DBNHKV010000356         4010 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_476049, whole genome shotgun sequence.
ACCESSION   DBNHKV010000356 DBNHKV010000000
VERSION     DBNHKV010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_476049"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="AC16PP_11365"
     CDS             complement(<1..344)
                     /locus_tag="AC16PP_11365"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014754441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR6482271.1"
                     /translation="MRNIPATSIKLVLCALVLAACTPSAGVIDKGEPATPQAAVAMPA
                     THARAVAAAVLRNGGNAVDAAVAAAFTLAVVQPEAGNIGGGGFMTIFMDGEARFLDYR
                     ETAPAAASRNMFL"
     gene            428..2719
                     /locus_tag="AC16PP_11370"
     CDS             428..2719
                     /locus_tag="AC16PP_11370"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6482272.1"
                     /translation="MSDHVRGRLLVLPLLLVLLALWGCSRTEEPAPAEPPEPSGAVSA
                     AEIERNSARNAYFGDLHVHTRLSFDAFIFGTRSTPDSAYAYALGEPIDHPAGFQMQLD
                     RPLDFLGVTDHAMYMGMLEEMSNPETRAGQHPIAVGIREAETPASRLEAFQAMFPYLR
                     KQLDGPDDLLDPAVLRSAWQETVAAAERHNDPGRFTSFVAYEYTAGGAERQNLHRNVV
                     FRGAAPELPFSSMDSGNPEDLWAWMDAQRGQGVEALAIPHNSNGSDGLMFELTRVDGS
                     PMDAAYAEQRMRNEPLVEITQVKGTSDAHPLLSPNDEWADFELMEVKVATVEPSRAAG
                     SYVREAYLNGLAMQAEQGFNPYRFGLIGSSDTHVGAGSFDEANYWSKVGIVDATSVQR
                     GSVPMDEPDADGNLYVGGDVATFQDWGASGLAGVWAEANTREDIYDAFRRKETFATTG
                     PRMQVRFFAGFGLEAAEQAGPDRISALYRDGVPMGGDLVGGDTDTAPHFFLWALSDPA
                     SARLQRLQVIKGWIDAEGRSQEKVFDVACSDGLEVDEATHRCPDNGARVNLTDCSISQ
                     NVGAAELESLWKDPEFDAGQMAFYYVRALENPTCRWSTWDAVRAGVEPRADLQATIQE
                     RVWSSPIWYVPGGLAGDQPEAEPAAASLVGVRWLWVGSTIGSADVEVAEPHRYNLLLH
                     SDGGAEFRLDCNQGRGGYEMEGERLRLGPIMSTRMACPEDSKDFEFGQQLDGVTGFSM
                     DGGALELRLNSEGDVMRFRAETD"
     gene            complement(2776..3420)
                     /locus_tag="AC16PP_11375"
     CDS             complement(2776..3420)
                     /locus_tag="AC16PP_11375"
                     /inference="COORDINATES: protein motif:HMM:NF024978.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HIR6482273.1"
                     /translation="MSAANLARSAFAAAVVAMISCSSPGIESFHDYNPNVNFARYQTW
                     SFISTNPMIVSTAAGAVNPLLEGRIMRAVRTDLAGKGFRYADNPENADFVVSFTVGSR
                     EQIKVDQYPASYQVGYSRYHRYGYGGYGYGMSYGTETRVRQYTEGQLAVDVFDVATRS
                     PAFHGSATKRISDSDRENPEALIAAVVGEALTGFPPGSAAGVAQPQLIPYEDQN"
     gene            3570..>4010
                     /locus_tag="AC16PP_11380"
     CDS             3570..>4010
                     /locus_tag="AC16PP_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482274.1"
                     /translation="MSLRKFWVVVSGLLLGSVALAGESPKFGDTVSLRLGGMIHKADA
                     TFSDTRIDRPPVELDLKGLGMDKRYDSFWAGLEWQFAKRWGLTLSYSDFSTDGERSVA
                     EGGNFGDLEWSVGAELTSEMDMKIYIVDVTWDFVKTERSHFGLGL"
BASE COUNT          684 a         1220 c         1380 g          726 t
ORIGIN      
        1 aggaacatgt tgcggctcgc cgcagcgggg gcggtctccc ggtagtccag gaacctggct
       61 tccccatcca tgaagatcgt catgaacccg ccgccgccga tgttgcctgc ctcgggttgc
      121 accacggcga gggtgaaggc ggccgcgacg gcagcatcca cggcgttgcc tccgttccgg
      181 agcacggcgg cggcgaccgc ccgggcgtga gtggcgggca tggcaacggc cgcttggggc
      241 gtcgcgggct cgcctttgtc aatgacgccc gcagagggtg tgcaagccgc gagcacgaga
      301 gcgcacagga caagcttgat actggtggct gggatgttgc gcatgtcccc cggcgctcgc
      361 tgccgacagt ggtctaagag cctgctacat tatctgaaac gtggagatct tcggaggggt
      421 gaggatgatg agcgatcacg tcaggggaag gctgctggtt ttgcccctac tgctcgtcct
      481 gctggcgctg tgggggtgct ctcgaaccga agaacccgct cctgccgagc cgcccgagcc
      541 gagcggtgct gtgtcggcgg ccgagatcga gcgaaactcc gcccgcaacg cctacttcgg
      601 cgacctgcac gtgcacacga ggctgtcttt cgacgctttc atcttcggca cgcgctccac
      661 gccggactcg gcttacgcct atgctctggg cgagcccatc gatcatcccg ccggtttcca
      721 gatgcagctg gaccggcccc tggattttct gggagtgacg gatcacgcca tgtacatggg
      781 catgttggag gagatgagca accccgaaac ccgggccggc cagcacccga tagcggttgg
      841 catccgcgag gcggagactc ctgcatcgcg tctagaggcg ttccaggcca tgtttcctta
      901 tctgcgcaag cagctcgatg gtccggacga tctgctcgat cccgcggtgc ttcgctctgc
      961 gtggcaggaa accgtggctg ctgccgagcg ccacaacgat ccgggccggt ttaccagctt
     1021 cgtggcgtac gaatacaccg cagggggagc cgagcggcag aaccttcacc gcaacgtggt
     1081 ttttcggggc gccgcccccg agctgccgtt ctccagcatg gactccggca acccggagga
     1141 cctgtgggcg tggatggacg cccagcgcgg ccagggcgtg gaagcgctgg cgattcctca
     1201 caactccaac ggctccgacg ggctgatgtt cgagctgacc cgtgtggacg gctcgcccat
     1261 ggacgcggct tacgccgagc agcggatgcg caacgagccc ctggtggaga tcacgcaggt
     1321 caagggcacg tctgacgccc atcccctgct ttcgcccaac gacgagtggg ccgacttcga
     1381 gctgatggag gtcaaggtgg cgaccgtgga gcccagcagg gcggccggca gctatgttcg
     1441 agaggcttac ctcaacggcc tggccatgca ggcagagcag ggattcaacc cgtaccggtt
     1501 cggcctcatc ggctcgtcgg acacccacgt tggcgcgggc tccttcgacg aggccaacta
     1561 ttggagcaag gtcggcatcg tggatgcgac ctccgtccag cgcggctccg tgcccatgga
     1621 cgagcccgac gccgacggca acctctacgt gggcggagat gttgcaacgt ttcaggattg
     1681 gggcgcgtcc gggctcgccg gggtatgggc ggaggcgaac acgcgtgaag acatatacga
     1741 cgcgttccgt cgtaaggaga ccttcgccac caccggcccg cgcatgcagg tccggttctt
     1801 cgcgggcttc gggctggagg cagcggagca ggcagggccc gaccggatca gcgcgcttta
     1861 tcgcgacggc gtgcccatgg gcggcgatct agtcggcggc gataccgata ccgcgccgca
     1921 ctttttcctc tgggctctga gtgacccggc gtcggcgcgc ttgcagcgtc ttcaggtcat
     1981 caagggatgg atcgacgcag agggaagaag ccaggaaaag gtgttcgacg tggcgtgctc
     2041 ggacggcctc gaggtcgacg aggcgaccca cagatgcccg gacaacggcg cccgcgtgaa
     2101 tctcacggac tgctccatca gccagaacgt gggcgccgcc gagctggaga gtctctggaa
     2161 ggatcccgag ttcgatgccg gccagatggc cttctactac gtgcgcgcgc tggagaaccc
     2221 cacctgtcgc tggtctacct gggacgcggt ccgggccggg gtggaaccca gagcggactt
     2281 gcaggctacg attcaggagc gcgtctggtc atcgccgatc tggtacgtcc ccggcggctt
     2341 ggcaggcgat caaccggaag ccgagccggc ggcggcaagc ctcgtcgggg tgcgttggct
     2401 ctgggtgggg tcgacgatcg gctcggcgga tgtcgaggtg gcggaacccc atcgctataa
     2461 cctgctgctg cactcagatg gtggcgcgga gttccgtctc gactgcaatc agggtcgtgg
     2521 cggctacgag atggaaggcg agcggctccg gctcggcccg atcatgtcga caaggatggc
     2581 ctgccccgag gactccaagg acttcgagtt cggccagcag ctcgacgggg tcactgggtt
     2641 ctcgatggat ggcggcgccc tggagctgcg cctgaattcc gagggcgacg tgatgcgctt
     2701 tcgcgccgaa accgactgat cgagcgcgcc tgagctccga tcaccggagc ccgcgcgtca
     2761 gtcgagtttg tcagatcagt tctgatcctc atacggtatc aactgaggct gcgccacgcc
     2821 ggcggctgac ccgggcggga agcccgtgag cgcctcgccc acgacggcgg cgatcagcgc
     2881 ttccggattt tcccggtcgc tgtcggatat ccgcttggtg gccgagccgt gaaaggccgg
     2941 gctgcgtgtg gctacgtcga agacgtccac ggcgagttgt ccttccgtgt actgccggac
     3001 gcgggtttcc gtgccgtagc tcatgccgta cccgtagccg ccgtaaccgt agcgatgata
     3061 gcggctgtag ccgacctggt agctggccgg atactggtcc accttgatct gctcccgcga
     3121 tccgacggtg aatgaaacga cgaagtccgc gttctccgga ttgtcggcat agcggaatcc
     3181 tttccctgcc aggtcggtcc gcaccgcgcg catgatccgg ccttccagca acggattgac
     3241 ggcgcccgcg gcggtggaga cgatcatggg attggtggaa atgaacgacc acgtctgata
     3301 ccgcgcaaag ttgacgttcg ggttgtagtc gtggaatgac tcgatccccg ggctgctgca
     3361 ggaaatcatt gcaacaacgg cggctgcgaa agcggatcgg gcaaggttgg cggcagacat
     3421 gcgtgctccc tttgtgtgtg ctttgtgacg cgggggcgat catagcggca aacggaggtc
     3481 ggcacacgtc cgcgatccgc agctgcacgg ctatgtataa tgccggttgc agaagtgctg
     3541 ggacgttgca aagcacgtag gagatgtcga tgagcttgcg gaaattctgg gtggtcgtgt
     3601 ctggccttct gttgggttct gtcgccctgg cgggtgagag ccccaagttc ggagacacgg
     3661 tctcgctgcg gctgggcggc atgatccaca aggccgatgc gaccttctcg gatacccgga
     3721 tagaccgccc gcccgtggag ctcgacctga aggggctggg catggacaaa cgctacgatt
     3781 cgttctgggc cggcctcgaa tggcagtttg ccaagcgatg gggcctgacg ctgagctact
     3841 ccgatttcag caccgacggg gaacgcagcg tggccgaggg tggcaacttc ggcgatctgg
     3901 agtggtcggt gggtgccgag ttgacgtccg agatggacat gaagatctac atcgtcgacg
     3961 tcacctggga tttcgtgaag accgaacgca gccatttcgg tctcggtctg
//
LOCUS       DBNHKV010000357         9681 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1183669, whole genome shotgun sequence.
ACCESSION   DBNHKV010000357 DBNHKV010000000
VERSION     DBNHKV010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9681)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1183669"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1877)
                     /gene="glmS"
                     /locus_tag="AC16PP_11385"
     CDS             complement(45..1877)
                     /gene="glmS"
                     /locus_tag="AC16PP_11385"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020190289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR6482275.1"
                     /translation="MCGIVGAVADRNVAPILLEGLQRLEYRGYDSAGLAVIDGAGEME
                     CRRRVGKVKELAGDLSARRCDGHMGIAHTRWATHGVPSENNAHPHVSNGRIALVHNGI
                     IENFEELREELQSEGYAFASETDSEVIVHLVDSYFDKGLSLLEAVRSAVKRLEGAYAI
                     GVIALADPDRIVAARMGSPLVVGKGLGENFIASDPLALRPVTDRFVFLEEGDLVEITR
                     DEISIWNVENESVIRASVRVELGHDDVDRGNYRHFMQKEIYQQPEVLAATLEGRIGRT
                     RVLEPAFGVQAGQIFDKVQAVTIVACGTSYYAGSIARYWIEELARIPCQVEIASEFRY
                     RQVAVTPGNLFVTISQSGETADTLAALRVAKELGYVSTLTICNVATSTLVRESDIALM
                     TQAGTEVGVASTKAFTTQLADLLLLTILIARRHGMDAAREQELVEALAGLPNAVEQVL
                     GLDGRIRVLAERFMDRSNALFLGRGPMFPVAMEGALKLKEISYIHAEGYPAGELKHGP
                     LALVDENMPVVAVAPNDELLEKVQSNLQEVRARGGALYVFADERTGFKEEPGVTVIPL
                     PPVHDTLAPIVYVVPLQLLAYHVAVLKGTDVDQPRNLAKSVTVE"
     gene            complement(1893..3284)
                     /gene="glmU"
                     /locus_tag="AC16PP_11390"
     CDS             complement(1893..3284)
                     /gene="glmU"
                     /locus_tag="AC16PP_11390"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR6482276.1"
                     /translation="MSSSPRLEVIVLAAGQGKRMNSRLPKVLHPLAGRPLLAHVLETV
                     DELEPAGIHVVIGHGADLVREAIGAAALRWVVQTEQLGTGHAVQQALPDIDDSAVVLV
                     LCGDVPMVTAQTLRRCVTGAGNGAMAVITAELSDPAQLGRILRGTDGAVRGIVEYKDA
                     SAAEKAISEINSGIIAAPAGILRDLLAEVRPDNAQGEYYLTDIVALAVSRGIEVLGLK
                     AERPEEVLGVNDRNQLAVLERRYQLERAGELMAAGATLADPSRLDVRGAVTVGADCFI
                     DVNVVFEGTVVMGREVSIGPGSVIRDSQLGDGVRVEAHTLVDGARVEAGCSLGPFARI
                     RPGTQLGEGVKIGNFVETKKAVLGAGSKASHLTYLGDAILGANCNVGAGTVTCNYDGV
                     DKHRTTIGDGVFVGTNSTLVAPVTLGDDAFVAAGSTITGDVEKGELAVGRGRQRNVQG
                     WVRPGRRDRADED"
     gene            complement(3455..3877)
                     /locus_tag="AC16PP_11395"
     CDS             complement(3455..3877)
                     /locus_tag="AC16PP_11395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014873131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HIR6482277.1"
                     /translation="MAMTFHCDIVSAEREIFSGRVTMLSAIGTIGELGILPGHAPLLT
                     GIRPGPVRLRMDNGEEEVFFASGGYLEVQPGVVTVLADTALRAEDIDEAAAAAAQQDA
                     ERALREQTADIEFSAAAAMLAEATARQRTLAELRKRRR"
     gene            complement(3889..5271)
                     /gene="atpD"
                     /locus_tag="AC16PP_11400"
     CDS             complement(3889..5271)
                     /gene="atpD"
                     /locus_tag="AC16PP_11400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR6482278.1"
                     /translation="MSSGRIVQIIGAVIDVEFSREDVPKVYDALKVSSTGLTLEVQQQ
                     LGDGVVRAIAMGPSDGLSRGLEVTNTQQPISIPVGEETLGRIMNVLGEPIDEKGPVNA
                     QQKMPIHRKPPSYEDQKGGNEILETGVKVIDLICPFAKGGKVGLFGGAGVGKTVTMLE
                     LIRNIATEHSGLSVFAGVGERTREGNDFYHEMEESGVLDKISLVFGQMNEPPGNRLRV
                     ALTGLTLAEQFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPNLAEEMGV
                     LQERITTTKTGSITSVQAVYVPADDLTDPSPATTFAHLDSTVTLSRAITDLGIYPAVD
                     PLDSTSRQLDPLVVGEEHYQVARGVQQVLQRYQELRDIIAILGMDELSEEDKQLVYRA
                     RKMQQFFSQPMFVAEPFTGQPGVYVSREDTVRSFKGILEGEYDHLPEQAFYMVGTIEA
                     AIEKAETLSA"
     gene            complement(5303..6163)
                     /gene="atpG"
                     /locus_tag="AC16PP_11405"
     CDS             complement(5303..6163)
                     /gene="atpG"
                     /locus_tag="AC16PP_11405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIR6482279.1"
                     /translation="MAVGKEIKKKIGSVENTKKITSAMQMVAASKMRRTQERMREGKP
                     YSEKIRAVIGHLANSNPEYRHVYMEPREISRIGYLVVSTDKGLCGGLNINLFRTLIRD
                     MAEWHAKGKEIDLGLIGNKAAAFFRSVGGNIVAAMNNVGEDPALADLIGGIKVMLDAY
                     EEGKIDRLFIASNEFVNTMTQRPVVRQLLPLDPETDDSMRHRWDYIYEPDARQLIEGL
                     VTRFMESQVYQAVVENGACEQAAKMVAMKSATENAEKLIDELTLIYNNARQAAITQEI
                     AEIVGGAAAV"
     gene            complement(6174..7718)
                     /gene="atpA"
                     /locus_tag="AC16PP_11410"
     CDS             complement(6174..7718)
                     /gene="atpA"
                     /locus_tag="AC16PP_11410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR6482280.1"
                     /translation="MQQLNPSEISDIIRQRIEGLDVRAQAQNEGTIVGVSDGIVRIHG
                     LADAQYGEMIEFDGGLYGMALNLERDSVGVVILGDYEGLSEGMTARCTGRILEVPVGR
                     ELLGRVVDSLGNPIDGKGPLNASETAPIEKVAPGVIARQSVDQPVQIGLKCIDSMVPI
                     GRGQRELIIGDRQIGKTAIAIDAIINQKDSGIRCVYVAIGQKMSTIANIVRTLEEHGA
                     MENTIVVAASASDPAPMQFIAPYSGCSMGEYFRDNGEDARIIYDDLTKQAWAYRQISL
                     LLRRPPGREAYPGDVFYLHSRLLERAARVNADYVEQYTGGKVKGRTGSLTALPIIETQ
                     AGDVSAFVPTNVISITDGQIFLETDNFNSGLRPAMSPGISVSRVGGSAQVPFIKKIAG
                     GIKLALAQYRELAAFSQFASDLDDATRRQLEHGRRVTELMKQKQYAPMSVAEMGVSLY
                     AANEGYLVDIAVDQVLEFEQGLLAYMNSEKADFMRRINETGDYDDGIVAEMRQGIEAY
                     KAQANF"
     gene            complement(7783..8322)
                     /locus_tag="AC16PP_11415"
     CDS             complement(7783..8322)
                     /locus_tag="AC16PP_11415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIR6482281.1"
                     /translation="MAELATLARPYANAVFATARDEGNLERWSRMLGFLAAAVREPEV
                     RRLIGSPDVSDEQKAFRLADVCGDELNDKARKLVQVLAGNKRLGLIDEILEQFEHKRA
                     QDEQTLDVEVVTAYPLSDDQAARLKQALGEKYAKEVNLSSVVNEDLIGGAIIRAGDTV
                     IDGSVRGRLIKLAESLQRI"
     gene            complement(8334..8804)
                     /locus_tag="AC16PP_11420"
     CDS             complement(8334..8804)
                     /locus_tag="AC16PP_11420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019694394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR6482282.1"
                     /translation="MNINWTIVGQSLTFFVFVWFCWKFIWPPIVKAMRERQEAIAEGL
                     ASSERAAKDLELAQERATDQLREAKDEAHKIIEQARGRANQMIDEAKNEAREEVERIR
                     EGARAEIDQEINRAKEALRGQVAALAVSGAERVLGSAIDAERHRELLDQLATDL"
     gene            complement(8874..9110)
                     /gene="atpE"
                     /locus_tag="AC16PP_11425"
     CDS             complement(8874..9110)
                     /gene="atpE"
                     /locus_tag="AC16PP_11425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HIR6482283.1"
                     /translation="MELEVLLYVAGGLMMGLGAIGAAIGVGVLGGKFLEGVARQPELL
                     PMLRTQLFIVLALVDAVPMIAVGIGLYTLFAVAG"
     gene            complement(9168..>9681)
                     /gene="atpB"
                     /locus_tag="AC16PP_11430"
     CDS             complement(9168..>9681)
                     /gene="atpB"
                     /locus_tag="AC16PP_11430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR6482284.1"
                     /translation="VFVWVFLMNLMDLIPVDWIPGLAAAMGISHMKIVPTTDVNITMA
                     MAGSVFGLIIYYSIKCKGVGGFLGELAFHPLPKAAAPANLFLEGVTLIAKPLSLGLRL
                     FGNMYAGEMIFILIALLFPAGIVYGLLGGVLQWGWAVFHVLIITLQAFIFAVLTVVYL
                     AQAHDVMEEH"
BASE COUNT         1672 a         3231 c         2957 g         1821 t
ORIGIN      
        1 gggggttcag ggaaccgccc ggcgcggctt agggccggaa cggactactc caccgtgacc
       61 gacttagcca gattccttgg ctggtccacg tccgtgcctt tgagcaccgc gacgtgatag
      121 gcgagcaatt gtaatggcac gacgtagacg atgggagcca gcgtgtcgtg aacggggggc
      181 agcgggatca ccgtgacccc gggctcttct ttgaagccgg tgcgctcatc ggcaaagaca
      241 tagagggcac cgccgcgcgc gcgcacttcc tgcaggttgg actgcacctt ctcgagcagc
      301 tcgtcgttcg gcgccacggc aacgaccggc atgttctcgt cgacaagcgc cagagggccg
      361 tgcttcagct ccccggcggg atagccctcg gcatggatgt aggagatctc cttcagcttc
      421 agcgcgcctt ccattgccac cgggaacatg ggcccgcgtc cgagaaacag cgcgttgctc
      481 cggtccatga agcgttccgc cagaacgcgg atgcgcccgt cgaggccgag cacctgctca
      541 acggcattgg gaagcccggc gagcgcctcc accagctcct gctctctggc cgcgtccatc
      601 ccgtgccggc gggcaatgag aatggtcagc agcaacaggt cggccagctg cgtggtgaac
      661 gccttggttg acgccacgcc cacctctgtc ccggcctggg tcatcagcgc gatgtcggac
      721 tcgcgcacca gcgtgctggt ggcgacgttg cagatggtga gcgtcgagac gtagccgagc
      781 tctttggcca cgcgcagcgc tgccagggta tccgcggtct cacccgactg ggagatggtc
      841 acgaacaggt tgcccggggt aaccgccacc tgccggtagc ggaattcgct ggcgatctcc
      901 acctggcagg ggatacgcgc gagctcctcg atccagtaac gcgcgatgga gcccgcgtag
      961 tagctcgtcc cgcaggccac gatggtcacc gcctggactt tgtcgaagat ctgcccggcc
     1021 tgcaccccga atgcgggctc gagcacccgg gtacggccta tgcgcccttc cagagtcgct
     1081 gcgagcactt ccggctgctg gtagatctcc ttctgcatga agtgccggta attgccccgg
     1141 tccacgtcgt cgtgaccgag ctctacccgg acgctggcgc ggatcaccga ctcgttctcc
     1201 acgttccaga tggagatctc atctcgggta atctcgacga gatccccttc ctccaggaac
     1261 acgaatcggt cggtcaccgg tctcaaggca agggggtcgg aagcgatgaa gttttctccc
     1321 agccccttgc caaccaccag cgggctgccc atgcgcgcgg cgacgatccg atcagggtcg
     1381 gcgagcgcga tgacgccgat ggcgtaagcg ccttccagcc gcttgaccgc ggacctgacg
     1441 gcctcgagca ggctcaatcc cttgtcgaaa tatgaatcca ccaggtgaac gatcacctcc
     1501 gagtcggtct cggacgcaaa cgcataaccc tcgctttgaa gctcttcgcg cagctcctcg
     1561 aagttttcga tgatgccgtt gtgcaccagc gcgatgcggc cgttggagac gtgggggtgg
     1621 gcgttgttct cgctgggaac gccgtgggtt gcccaacgcg tgtgggcaat tcccatgtga
     1681 ccatcgcatc gccgggcgct caggtctccg gcgagctcct tgaccttgcc gacgcgacgc
     1741 cggcactcca tctcacccgc cccgtcgatc acggcaaggc cagcggagtc gtacccgcga
     1801 tactccagcc gctgaagtcc ttccaagagg atgggcgcca cgttgcggtc ggcgacggcc
     1861 cctacgatcc cgcacatgcc aggctcctca tgctaatcct cgtccgcgcg gtcgcgccgg
     1921 ccaggtcgta cccagccctg aacgttacgc tgtcggcccc gccccacggc cagctcgccc
     1981 ttctcgacat cgccggtgat cgtggacccc gccgccacga aagcatcgtc gcccagcgtg
     2041 accggggcga ccagcgtgct gttggtgcct acgaagacgc cgtcgccaat ggtcgttcgg
     2101 tgcttgtcga caccgtcgta gttgcaggtc accgtgccgg cgcccacgtt gcagttggcc
     2161 ccgaggatgg catcaccaag ataggtcagg tgactggcct tgcttcctgc acccagcacg
     2221 gccttcttgg tctcgacgaa gttgccgatc ttcacgcctt cgcccagctg cgtgcccggt
     2281 cggatccggg caaagggccc caggctgcaa ccggcttcga ccctggcacc gtccaccagg
     2341 gtgtgggctt cgacgcgcac accgtcgccc agctgagaat cccgaatgac cgagccggga
     2401 ccgatgctca cttcccggcc catgaccacg gtgccctcga aaacgacgtt gacgtcgatg
     2461 aagcagtcgg cgcccacggt gaccgcgccg cgcacgtcca gtcgcgacgg atccgccagg
     2521 gtggccccgg cggccatcag ctcgccggcc cgctcgagct ggtagcgccg ctcgaggacg
     2581 gccagctggt ttcggtcatt cacgcccagc acctcttcgg gccgctccgc cttcagaccc
     2641 agcacctcga taccgcggct gaccgcaagg gccacgatgt cggtgaggta gtactctccc
     2701 tgagcgttgt ccgggcggac ctcggcaagc aggtcccgca aaatgcccgc aggcgcggcg
     2761 atgattcccg aattgatctc cgagatggcc ttctcggcag cgctggcatc tttgtactcc
     2821 acgatgccgc gcacggcgcc gtcggtacct cgcaggatcc tccccagctg cgccggatcg
     2881 ctcagctccg cggtgatgac ggccattgcg ccgttgccgg cgccggtgac gcagcgcctg
     2941 agcgtctggg ccgtcaccat cggcacgtcg ccgcacagaa ccagaaccac ggctgaatcg
     3001 tcgatgtccg gcagggcctg ctgcacggcg tggccggtgc ccagctgctc ggtctgcacc
     3061 acccaccgca gcgccgccgc gccaatggcc tcacgcacca gatcggcgcc atgaccgatg
     3121 accacgtgaa tgccggcggg ctctagctcg tcaacggtct ccaggacgtg ggcgagcagg
     3181 ggacgcccgg ccagcgggtg cagcactttc ggtaaccgcg agttcatgcg cttaccctgg
     3241 ccggcggcca gcacgatgac ttcaaggcga gggctggaag acataaccat gttgagcaga
     3301 aacagtccct ggtttcaggc cgggccgaac cgcacccgcg tcacgggcgg ccgaacctct
     3361 atgctctgac gattatagaa ccggggaacg aataaagggt gacacagatc acccctgcat
     3421 tggacaagaa ccttgggcag attcgcccgg cgcgtcagcg ccggcgcttg cgaagctctg
     3481 ccagcgtcct ctgccgcgcg gtcgcctcgg caagcatggc agcggcagcg gaaaattcga
     3541 tatccgccgt ctgctcccgc aatgcccgct ccgcatcctg ctgagcggca gcagcggcgg
     3601 cctcgtcgat gtcttccgcg cgcagcgccg tgtcggcaag aacggtgacc acccctggct
     3661 ggacctcgag ataccctccg gaggcgaaga agacctcttc ctcgccattg tccatgcgca
     3721 gccggaccgg ccccggacgg atgcccgtca gcagcggggc gtggccgggc aggatgccca
     3781 gctcaccgat ggtcccgatg gcgctcagca tggtcacccg gccggagaag atctcgcgct
     3841 ccgcgctgac gatatcgcaa tggaaggtca tggccatggc cgctctcctt aggcgctaag
     3901 cgtctcggct ttctcgatcg ccgcttcgat ggtgcccacc atgtaaaacg cctgctccgg
     3961 aaggtggtcg tattctccct cgagaatgcc cttgaagctg cgcaccgtat cctcccggga
     4021 gacgtacacg cccggctggc cggtaaaggg ctcggcgacg aacatgggct gcgagaagaa
     4081 ctgctgcatc ttgcgcgcgc gatagaccag ctgcttatcc tcctcggaaa gctcgtccat
     4141 cccaaggatg gcgatgatgt cgcggagctc ctggtagcgc tgaagcacct gctgaacgcc
     4201 ccgggccacc tgatagtgct cctcaccgac caccagcggg tcgagctgac ggctcgtgga
     4261 atccagcgga tccacggcgg ggtagatgcc gaggtccgtg atggccctgg agagcgtcac
     4321 cgtggaatcc aggtgcgcaa aggtggtggc cggagacggg tcggtgaggt catcggcggg
     4381 cacgtaaacg gcctgcacgg acgtgatcga tccggtcttg gtggtggtga tccgctcctg
     4441 cagcacgccc atctcctcgg ccaggttcgg ctggtagcct accgcggacg gcatccggcc
     4501 gagcagcgcg gacacctcgg ttcccgccag cgtgtagcgg tagatgttgt ccacaaagag
     4561 gaggacgtcg cgaccttcgt cgcggaactg ctcggccagg gtcaggccgg tgagcgctac
     4621 ccgcagacgg ttacccggcg gctcgttcat ctgcccgaaa acgagcgaga tcttgtcgag
     4681 cacgcccgac tcctccatct cgtggtagaa gtcgtttcct tcccgggtcc gctcgcccac
     4741 accggcgaag accgacaggc cggagtgctc ggtggcgatg ttgcggatca gctccagcat
     4801 ggtgaccgtc ttgccgacgc cggcaccgcc gaacaggccc accttgccgc ccttggcgaa
     4861 cgggcagatg aggtcgatga ccttgacgcc cgtctcgaga atctcgttgc cgcctttctg
     4921 gtcctcgtat gagggcggct tgcggtgaat gggcatcttc tgctgcgcgt taactggccc
     4981 cttctcgtcg atgggctcgc cgagcacgtt catgatccgg ccaagggttt cttcacccac
     5041 ggggatggag atgggctgct gggtattggt cacctccaga ccccgcgaga gaccgtccga
     5101 gggccccatg gcaatggcac gaaccacccc gtcaccgagc tgctgctgca cctcgagggt
     5161 gagccccgtt gaggaaacct taagggcgtc gtagaccttt gggacatcct cccgcgagaa
     5221 ttcgacgtca atcaccgcgc cgataatctg tacgattcgt ccgctgctca tgctttgtgc
     5281 ctcttcaatc taatctgtct atctaaacgg ccgcggcccc gccgacgatc tccgcaatct
     5341 cctgagtgat cgccgcctgc cgagcgttgt tgtaaatcag ggtcagctcg tcgatgagct
     5401 tctccgcgtt ttccgtggcg ctcttcatgg cgaccatctt agccgcctgc tcgcaggcac
     5461 cgttctccac caccgcctgg tagacctggg attccatgaa gcgggtaacc aggccctcga
     5521 tgagctggcg cgcgtccggc tcgtagatgt agtcccagcg atgcctcatg ctgtcgtcgg
     5581 tttccgggtc caggggcagg agctgccgga ccaccgggcg ctgggtcatc gtattgacga
     5641 actcgttgct ggcgatgaac agccggtcga tcttgccttc ctcgtaagcg tcgagcatga
     5701 ccttgatccc gccgatgagg tcggccagcg caggatcctc gccgacgttg ttcatggccg
     5761 cgacgatgtt tccaccaacc gagcggaaga acgccgccgc cttgttgccg atcaggccaa
     5821 ggtcgatctc cttgcccttg gcatgccact ccgccatgtc ccggatcagg gtgcggaaca
     5881 ggttgatgtt gaggccgccg cagagtccct tgtccgtgga gaccacgagg tagccgatcc
     5941 ggctgatctc gcgaggctcc atgtagacgt gccggtactc cgggttggag ttcgccagat
     6001 ggccgatgac cgcgcggatc ttttccgagt agggcttgcc ttcccgcatc cgctcctggg
     6061 tgcggcgcat cttgctcgcc gcaaccatct gcatcgcgct cgtaatcttc ttggtgttct
     6121 cgacgctgcc gatctttttc ttgatctctt tgccgaccgc catatccgct gccttagaag
     6181 ttcgcttgtg ctttgtaggc ttcgatgccc tggcgcatct ccgcaacgat gccgtcgtcg
     6241 tagtcgccgg tctcgttgat gcgccgcatg aagtcggctt tctcggagtt catgtaggca
     6301 agcaggccct gctcgaactc gagcacctga tcgaccgcga tgtccaccag gtagccctcg
     6361 ttcgccgcat aaaggctcac gcccatttcc gctacgctca tcggcgcgta ctgcttctgc
     6421 ttcatgagct ccgtgactcg ccggccgtgc tcgagctggc gccgcgtcgc gtcgtcaagg
     6481 tctgaagcaa actgagagaa cgctgcgagt tcccggtact gggcaagggc gagcttaatg
     6541 ccaccggcaa tcttctttat gaagggcacc tgggcggatc cccccacccg ggataccgag
     6601 atgcccgggc tcatggcagg gcgcagcccc gagttgaagt tgtccgtctc taagaagatc
     6661 tgcccatcgg tgatggagat gacgttcgtc ggcacgaacg cggatacgtc ccccgcctga
     6721 gtttcgatga tcggcagcgc cgtcagggac ccggtccggc ccttgacctt accccccgtg
     6781 tactgctcca cgtaatcggc gttcaccctt gccgcacgct cgagcaaacg cgagtgcaga
     6841 tagaagacgt cgccgggata agcctcgcgg cccggcggcc ggcgcagcag gagcgagatc
     6901 tgccggtagg cccaggcctg tttcgtgaga tcgtcgtaaa tgatccgggc gtcttcaccg
     6961 ttatcccgga agtattcgcc catgctgcag ccggagtaag gcgcgatgaa ctgcatgggc
     7021 gccggatcgg atgcgctagc agccacgacg atggtgttct ccatggcgcc gtgctcttcg
     7081 agggtccgga cgatgttggc aatcgtcgac atcttctgcc cgatggccac gtagacacaa
     7141 cgaatgccgc tgtccttctg gttgatgatg gcgtcgatgg cgatggccgt cttgccaatc
     7201 tgcctgtcgc cgatgatgag ctcgcgctga ccccggccga tgggcaccat ggagtcgata
     7261 cacttcagcc cgatctgaac gggctggtcc acggactgcc gcgcaatcac gccgggggca
     7321 accttttcga tgggcgcggt ttccgaggcg ttcagcggcc ctttgccgtc aatggggttg
     7381 ccaagggaat ccaccacccg gccgagcagc tcgcggccca ccgggacttc gagaatccgc
     7441 ccggtgcatc gagccgtcat cccctcggag agaccctcgt agtcgccgag gatcaccacg
     7501 ccgacagagt cgcgctcgag gttgagcgcc attccgtaga ggcccccgtc gaactcgatc
     7561 atctcgccat actgggcgtc ggcaaggccg tggatgcgga cgatgccatc tgacacgccc
     7621 acaatggtgc cttcgttctg cgcctgcgcg cgtacgtcca atccttcaat ccgctgcctg
     7681 atgatgtcgc tgatctcgga aggattcagt tgttgcattt cgaattcctc agtctctttc
     7741 ttggagcctc gacggtgtcg ttcccgccga aggcctctgt attcagatac gctgcagcga
     7801 ctctgccagc ttgatcaaac gcccgcgcac ggagccgtcg atgacggtat ccccggccct
     7861 gatgatggcg ccgccaatca gatcttcgtt cacgacgctg gagaggttca cttccttggc
     7921 gtacttttcc cccagcgcct gcttcagcct ggcggcctga tcgtcgctca aggggtaggc
     7981 ggtcaccacc tcaacatcga gggtctgctc gtcctgggcg cgcttgtgct cgaactgctc
     8041 gagtatctcg tcgatgagcc cgagccgctt gttcccggcc agcacctgga cgagcttgcg
     8101 cgccttgtcg ttcagctcgt cgccgcaaac atcggcaagc cggaacgcct tctgctcgtc
     8161 gctcacgtcc ggtgagccga tcagccgacg aacctcaggc tcccgaacag ccgcggcgag
     8221 aaagccgagc atccgggacc agcgctccag gttgccttcg tcgcgagccg tggcaaacac
     8281 cgcgttggca taagggcggg cgagggttgc tagttctgcc attggtcaga ccgtcacagg
     8341 tcggtggcaa gctggtcgag cagctcgcgg tgccgctcgg catcgatggc ggaacccagc
     8401 acccgctcgg cgccggaaac cgccagcgcg gcaacctgac cccggagcgc ttctttcgcg
     8461 cggttgatct cctgatcgat ctccgcccgg gcgccttccc tgatgcgttc gacctcttcg
     8521 cgcgcctcgt tcttggcctc gtcgatcatc tggttggccc ggccccgggc ctgctcgatg
     8581 atcttgtggg cttcgtcctt ggcctcgcgc agctgatccg tggcgcgctc ctgggcaagc
     8641 tccaggtcct tggccgcacg ctcggacgat gccagcccct cggcaatggc ttcctgacgc
     8701 tcgcgcatgg ctttgacgat gggcggccag atgaatttcc agcagaacca gacgaaaacg
     8761 aaaaacgtca ggctctgccc gacgatggtc cagttgatgt tcacgaactc acctcttcga
     8821 ttcgtttcga cagaccgtca cggcacaccg tggccggcag ttccgtcggg tggctaacct
     8881 gccaccgcaa agagggtgta cagaccgata cccaccgcga tcatgggcac ggcgtcaacc
     8941 agcgccagca cgatgaagag ctgggtccgg agcatcggaa gcagttcggg ctggcgcgcg
     9001 acgccttcca gaaacttgcc gccgagaaca cccacgccga tggcggcccc aatggcgccc
     9061 agacccatca tgagtccacc ggctacatat aagagaactt ctagttccat gatcactccc
     9121 agttacgagt tgttgtttcc gctctcggaa tttcccatca ggagagctta gtgctcttcc
     9181 atcacgtcgt gggcctgtgc caggtaaacc accgtcagga ccgcaaagat gaaagcctgc
     9241 agggtgatga tgaggacgtg gaagaccgcc catccccact gcagcacgcc tcccagcagc
     9301 ccgtagacga tgcccgccgg aaacagcagc gcgatgagga tgaagatcat ctcccccgca
     9361 tacatgttgc cgaacagtcg caggcccaga gaaagcggtt tggcaatcag ggtcaccccc
     9421 tctagaaaca ggttcgccgg cgcggccgcc ttgggcaagg ggtggaaggc cagctcgccg
     9481 aggaaacccc cgacaccttt gcatttgatg ctgtagtaga tgatgaggcc gaacaccgac
     9541 ccggccatgg ccatggtgat gttcacgtcc gtggtcggaa cgatcttcat gtggctgatc
     9601 cccatggctg cggccagccc cgggatccaa tccaccggga tcaggtccat caggttcatg
     9661 agaaacaccc agacgaagac g
//
LOCUS       DBNHKV010000358         4893 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_973307, whole genome shotgun sequence.
ACCESSION   DBNHKV010000358 DBNHKV010000000
VERSION     DBNHKV010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_973307"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="AC16PP_11435"
     CDS             <1..801
                     /locus_tag="AC16PP_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482285.1"
                     /translation="LRNAGIEDSWFDQLRSAVAKEWYFRGANHLAGMSRETNAAAREA
                     FEKLHELEPESAIGSSNVALTYWNDFAAGWGPDRDEALAHAIEWAKLARSYDRNDGIG
                     DAVYAYEQLGRRNYEGAMEAARRSVRVRPNCPISLGVLAQVENCSGNHDAAAALAEIA
                     QSKVEVPFPWVCNVLATAQRDGGQLERSLLTLERALQAAPDDVDLLVTKCSDHALLSD
                     LESAESAASRILSVEPEWSTAQYAAEALYRSPDELDRVVAALRSAGLP"
     gene            918..2033
                     /locus_tag="AC16PP_11440"
     CDS             918..2033
                     /locus_tag="AC16PP_11440"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR6482286.1"
                     /translation="MEGLETPWSMAWLPDGRMLITERRGELLRFDPEDESLTEIEGVP
                     AVRAEGQGGLFDVVLHPRYAETGWVYLAYAYLDAESGRSSTAILRARLEGDALIDQEP
                     LYRAEQDFGLVSDLKSSVKQMLGKPPGRDSRAHYGGRMVFDEAGYLYFSVGDRGSRDL
                     FPQDLTRDAGKIYRIHDDGRIPEDNPFLHREDAKPAIYAYGLRNPQGMARHPGTGKIW
                     IHEHGPRGGDELNVIEPGANYGWPILSHGVNYSGSEFAEGTEREGFRSPAWHWTPSIA
                     PSGMAFVTSPRYPEWRGGLLAGSLKFGYLVLCRLDGDRVTGQEIVVEDLGRLRDVREG
                     PDGFIYVAIDGRGIVRILSSVGAGESAADGAETIPGL"
     gene            complement(2030..2779)
                     /locus_tag="AC16PP_11445"
     CDS             complement(2030..2779)
                     /locus_tag="AC16PP_11445"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6482287.1"
                     /translation="MTEELQQAHEALIRGEGFTVLSSLMTEGEAARVRDSILNHLEQA
                     VDERDGVARLSNLLAVDTSFDAYVTNPMLLGLAHRMLGPDARLAALGARVLMPGCELG
                     GLHVDYPYWAMDPGMPVEPALMLQVIWMLEPFTEDNGGTWVAPGSQAWGEYPSEKRFA
                     DSAVQITGNAGDAVVSHGLLWHRTAKNRTDEPRVALLINYSQLAIQPMTPMGPFTDEY
                     RAGASAELRALLGFDHGRALRNRAMNVAREG"
     gene            2859..3683
                     /locus_tag="AC16PP_11450"
     CDS             2859..3683
                     /locus_tag="AC16PP_11450"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6482288.1"
                     /translation="MDTDLATQVEQLADRVRGLSSASVDKPVLVYFDIIGICWPIRCL
                     LHLKGVDYELVQVPIQAWAFRDANGDQVLKACFRNGHMPLYVDPDVYLTQSSVIMYYL
                     GEKHGMMGDGAAEKLAVMDVLSHAYDALFHWTGLFPVNVRMNTPEDVVTARLNAFLGD
                     GSWGLVSNGYRNNLDPFERYLDANPHRDSGFIVGSRLSIADLHAFNVLCNWYKAFDRE
                     RFTAEYPRLDEYIRRIAAIPEVDDYIRTAQEPTLWFPLPQFALRLTSHEELVGLTA"
     gene            3778..4371
                     /locus_tag="AC16PP_11455"
     CDS             3778..4371
                     /locus_tag="AC16PP_11455"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6482289.1"
                     /translation="MSDEDHEQVSIYPFSDEKREELLSKARECVFYWTTKDGWAVGVV
                     HAFVWHDGRAWITCGAHRHRVSAIRRDPRCSVVVSGSAAPDGPNGAITIKGRAIIHDD
                     EETKRWFYPALARGPYGRAGGALTEDEEAAAEDFVRRLDSPLRVIIEIVPEKWIMLDS
                     DKMAKDTAGELTDEERGPLLEADAKRMPDELKRRGIE"
     gene            4417..>4893
                     /gene="gloA"
                     /locus_tag="AC16PP_11460"
     CDS             4417..>4893
                     /gene="gloA"
                     /locus_tag="AC16PP_11460"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HIR6482290.1"
                     /translation="MSAETADYRMNQTMLRIKDPEVSIPFYRDVLGMTLLDRYDFPDM
                     EFTLYFMGYPDDTDGQVPEGRADKAGWVFGRKALIELTHNWGTEADDAFAGYHNGNED
                     PRGFGHIGISVPDVYAACERFEQLGVDFVKRPDDGSMKGLAFIRDPDGYWIEVLSSN"
BASE COUNT          856 a         1507 c         1656 g          874 t
ORIGIN      
        1 ctgcgcaatg ccgggatcga agactcgtgg tttgaccagc ttcgaagcgc ggtagccaag
       61 gagtggtatt tccgtggtgc gaaccatctg gctggtatgt cccgcgagac caacgcggcg
      121 gcccgcgaag cgttcgagaa gctgcacgag ctcgagccgg agtccgccat cggctcgagc
      181 aacgttgcgc tcacctactg gaacgacttt gctgccggtt ggggcccgga tcgggacgag
      241 gccctggccc acgcgatcga gtgggccaag cttgcgaggt cttacgatcg caatgacggg
      301 atcggggatg ccgtgtacgc ctacgagcag cttggtcggc gaaattacga gggggccatg
      361 gaagcggcgc gccgcagcgt cagagtccgg cccaactgcc cgatctccct gggcgtgctc
      421 gctcaggtcg agaactgcag cggcaatcac gatgcggctg ccgctctggc ggagattgcc
      481 caatccaagg tggaggtgcc tttcccatgg gtatgtaacg tcctggccac ggctcagagg
      541 gacggtggtc agctcgaacg ttctttgctg accctcgagc gcgctttgca ggccgcgccc
      601 gatgacgttg acctgcttgt cacgaagtgc agtgaccatg cgctgctgtc tgatctcgag
      661 tccgctgaat ccgctgcaag caggattctc tcggtggaac ccgagtggtc gacggcccaa
      721 tacgcagcag aagcgctcta cagatcgccc gatgagctcg atcgggtggt tgcggcgctg
      781 cgatctgcgg gtttgccatg aggggccgtt ttggaggttg gttcgaagct cggcctttcc
      841 ggagggccgt ctggggcctg cttctgtggt tgtgcgtcgg cctttcccac gcgggaacct
      901 atgaggtgga gaccgtcgtg gaaggtctgg aaacgccctg gagcatggcc tggctgccgg
      961 acggccgcat gctcatcacg gagcgccggg gtgagctgct gcgcttcgac cccgaagacg
     1021 aatcgcttac cgagatcgag ggtgttcccg cggtgcgggc cgagggtcag ggcgggctct
     1081 tcgacgtggt gctccatccg cgctacgcgg agaccggctg ggtctacctg gcatacgcct
     1141 acctcgatgc ggagtcgggc agaagcagca cggcgatcct tcgggcgcgg ctcgagggag
     1201 atgcgctgat agaccaggaa cccctttacc gcgcggagca ggacttcgga ttggtctcgg
     1261 atctcaagag ttccgtcaag cagatgctcg gcaagccgcc tgggcgcgat tcgagagccc
     1321 actacggcgg ccgcatggtc ttcgatgaag cgggatatct gtacttctcc gtgggagacc
     1381 ggggcagccg ggacctgttt ccccaggacc tgacccggga cgcgggtaag atctaccgga
     1441 tccacgacga tggccggatc cccgaggaca acccgttcct gcatcgggag gatgcgaagc
     1501 cggccatcta cgcctacggg cttcgcaatc cccagggcat ggcccgccat cccgggacgg
     1561 ggaagatctg gattcacgag cacggtccca ggggcggcga cgagctcaac gtgatcgagc
     1621 ccggggccaa ttacgggtgg ccaatcctca gccacggtgt gaactacagc ggcagcgagt
     1681 ttgccgaagg caccgagcgg gaggggtttc gctcaccggc ctggcactgg acgccgtcca
     1741 tcgcgccctc cggcatggct ttcgtgacct cgccgcgata tcccgaatgg cggggtggcc
     1801 tgctggccgg ctcgctgaag ttcggttacc tggtgctgtg ccggctggac ggtgatcgcg
     1861 tgaccgggca ggagatcgtt gtcgaagatc tgggtcgact ccgcgacgtc cgggaagggc
     1921 cggatggttt catctacgtg gccattgacg gtcggggcat cgtccggatc ctttcgtccg
     1981 ttggcgccgg cgagtcggcg gccgacggag cggaaacgat cccggggctc taaccctccc
     2041 gggcgacgtt catggcccga ttcctcaggg ccctgccgtg gtcgaacccg agcagcgccc
     2101 gcagctcggc cgatgcgcct gcccggtact cgtccgtgaa ggggcccatg ggtgtcatgg
     2161 gctggatggc gagctgggag tagttgatga gcagggccac cctgggctcg tccgtccggt
     2221 tcttggccgt tcggtgccac agcagaccgt ggctcaccac ggcgtcaccc gcgttgccgg
     2281 tgatctgcac ggcggaatcc gcaaaacgtt tctccgacgg gtactcgccc caggcctggc
     2341 tgccgggcgc cacccaggtg ccgccgttgt cctcggtgaa gggctcgagc atccagatca
     2401 cctggagcat cagggccggc tccaccggca tgccgggatc catggcccag taaggatagt
     2461 ccacgtgcag gccgccgagc tcgcagcccg gcatcagaac ccgggcccca agggcggcca
     2521 gccgcgcgtc ggggccgagc atccggtggg cgagtcccag cagcatggga ttggtaacgt
     2581 acgcatcgaa ggacgtatcc accgccagca ggttcgaaag cctcgcgacc ccgtcgcgct
     2641 cgtccacggc ctgttccaga tgattgagga tcgagtcccg aacgcgggcc gcttcgccct
     2701 ccgtcatcag gctggagagg accgtgaagc cttcgccccg gatcagcgct tcgtgtgcct
     2761 gttgcagctc ttcggtcatt ttcgagcccc ctgacagggg agagactagc acggcatggg
     2821 gtatgcttcg ctccaaagca agggaagggg aacgggccat ggatactgat ctcgccacgc
     2881 aggtcgagca gcttgcagac cgagttcgcg gtctgtcgtc cgcaagcgtc gacaagcccg
     2941 tgctcgtcta cttcgacatc atcggcatct gctggcccat acgctgcctg ctgcacctga
     3001 agggcgtcga ttacgagctg gtgcaggtgc cgatacaggc ctgggccttt cgcgacgcga
     3061 atggtgatca ggtgctcaag gcctgcttcc gcaacgggca catgcccctt tacgtcgatc
     3121 cggacgtcta cctcacccag agcagcgtga tcatgtacta cctgggcgag aagcacggga
     3181 tgatgggtga cggggcagcg gagaagctgg cggtgatgga tgtgctgtcc cacgcgtacg
     3241 acgcgctctt tcactggacc ggtctctttc cggtaaacgt caggatgaac acgcccgaag
     3301 acgtcgtgac ggcgcgtctt aacgcgtttc tcggcgacgg cagctggggg ctcgtctcca
     3361 atgggtatcg caacaatctg gacccttttg agcgttacct ggatgccaac ccccaccggg
     3421 acagcggctt catcgtcggt tcccggctct ccattgccga cctgcacgcc ttcaacgtgc
     3481 tgtgcaactg gtacaaggcc ttcgaccggg aacggttcac ggccgagtac ccgcgtctgg
     3541 acgaatacat ccggcgcatc gcggcgatac ccgaggtcga cgactacatc aggaccgcgc
     3601 aagagccgac cttgtggttt ccgctgcctc agtttgccct gcggctgacg tcccacgagg
     3661 agctcgttgg tcttaccgcc tgatcgttcc gagccctgcg tcgaggcttt gaccccgcca
     3721 cgggcgccgc actatccttt cggcctggct cgatcgggtc gattaagggg agggacgatg
     3781 tccgacgaag accacgaaca ggtttccatc tacccgtttt ccgacgagaa gcgcgaagag
     3841 ctcttgagca aggcgcgcga gtgcgtgttc tactggacca ccaaggacgg ctgggccgtg
     3901 ggcgtggtgc atgccttcgt ctggcacgac gggcgggcct ggatcacctg cggtgctcat
     3961 cggcaccggg tgtccgccat ccggcgggac ccaagatgct ccgtggtggt cagcggcagt
     4021 gccgcccctg acggtcccaa cggcgccatc acgatcaaag ggcgcgccat catccacgac
     4081 gacgaggaaa ccaagcgctg gttctacccg gcgctggccc ggggtcctta cggccgggcc
     4141 gggggcgcgc tgaccgagga cgaggaagcg gcggcagagg atttcgttcg gcgtctggat
     4201 tctccgctgc gggtgatcat cgagatcgtc cccgagaagt ggatcatgct cgacagcgac
     4261 aagatggcga aggacacggc gggggagctg acggacgagg agcgcgggcc gctgctcgag
     4321 gcggatgcca agcgcatgcc ggatgagttg aagcgccgcg gaatcgaata gacgaagccg
     4381 gcgccgagtg ggtgaacatg agaatgagag gtagacatga gcgcggaaac cgctgactac
     4441 agaatgaacc agacgatgct gcggatcaaa gatccggagg tatcgatccc cttttatcgc
     4501 gacgtgctgg gaatgaccct gctcgatcgc tacgacttcc cggacatgga gttcacgctc
     4561 tatttcatgg gctatcccga cgacaccgac gggcaggtgc ctgagggccg ggcggacaag
     4621 gccggctggg tgttcgggcg caaagcgctg atcgagctga cccacaactg gggcacggaa
     4681 gccgacgacg cctttgccgg atatcacaac ggcaacgagg acccccgcgg attcgggcac
     4741 atcggcatct cggtccccga tgtgtacgcg gcctgcgagc gcttcgagca gctcggggtg
     4801 gacttcgtga agcgtccgga cgacggttcc atgaaggggc tcgccttcat ccgggacccg
     4861 gacggctact ggatcgaggt gctgtcgtcg aac
//
LOCUS       DBNHKV010000359        13526 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_688767, whole genome shotgun sequence.
ACCESSION   DBNHKV010000359 DBNHKV010000000
VERSION     DBNHKV010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13526)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13526)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13526
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_688767"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /gene="grpE"
                     /locus_tag="AC16PP_11465"
     CDS             complement(<1..418)
                     /gene="grpE"
                     /locus_tag="AC16PP_11465"
                     /inference="COORDINATES: protein motif:HMM:NF013212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR6482291.1"
                     /translation="MTDDKPDAADLDGGSEESHTEVVESDPLEQEAPPPSASEAEIDD
                     PLAEAERQVAGLKDQVLRAQAEMENVRRRAARDVENAHKYALERFTSDLLPVLDSLEK
                     AVEASGESAGEAAGAIKEGVELSLKLFLTTLEKAGVI"
     gene            complement(517..1566)
                     /gene="hrcA"
                     /locus_tag="AC16PP_11470"
     CDS             complement(517..1566)
                     /gene="hrcA"
                     /locus_tag="AC16PP_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR6482292.1"
                     /translation="MARKQVEDNVDDRAQLLFKLLVERYLADGSPVASKKLAAFPEVE
                     VSPATVRNIMAELEDRGLVRSPHTSAGKVPTNLGLRFFVDSLLAVQPLGEEQIHRLEL
                     ELNPDLSPQELVESASNLLSHLTRMTCVVTLPRRDQVALRHVEFLRLSGDRVLVILVL
                     NDREVQNRVIHTDREYEDTELAQAANFINREFGGLSLVSIRDALLSSMQADKDGMDRL
                     MQTALDMAGRVFSESDQPLGKELVIAGESNLLDVSGDMATVRTLFDAFSRKGSILHLL
                     DRCLDSDGIQLFIGEESGYHILGDMSLVTAPYEVNGELAGVLGVVGPTRMAYQDVIPV
                     VDVTARVLGAAMNYG"
     gene            1663..3315
                     /gene="recN"
                     /locus_tag="AC16PP_11475"
     CDS             1663..3315
                     /gene="recN"
                     /locus_tag="AC16PP_11475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR6482293.1"
                     /translation="MLRELFVRDFALVESLEIGLCEGLTVITGESGAGKSILLGALGL
                     VLGDRADTDTIRPGAKRADVTAEFDLSGQPAAQAFLAEHDLEDADHPERCLVRRVVNQ
                     EGRSRAFINGAAVTRALLREFAEDLVDIHGQHEHQRLAEPATQLKLLDDYGVDAALLE
                     SCRESYRFWQRAVAEADALEAALASKQDRASLLSYQLEELAALGLEPGEFETLESEHK
                     RLSQAQDLRETVGHGLAVLEEADVLGRVSGALANLDDEHPALQAARDLLSSADSLVTD
                     AVRELRRYDDSLDVDEGQLGLLEERLTSVVDLARKHRVQPDRLVEHMALLQAELDAIS
                     TDRGSLDALREAAAGHEAEFRSTAGDVSLQRHEAAVHFASAVSGCMQNLGIGGGELRL
                     AFKEGESERGLESVEYQVMTNPKYPEAPLARIASGGERARISLAIQVVAAEKSALPCL
                     VLDEADVGIGGTTADVLGRLLRSLARHTQVICVTHAPQVAALGNHHLRVTKSSDQDTR
                     IEPLSEDTRIDEVARMLAGSGVTDKSRDYARTLLDEAHQALH"
     gene            3367..3768
                     /locus_tag="AC16PP_11480"
     CDS             3367..3768
                     /locus_tag="AC16PP_11480"
                     /inference="COORDINATES: protein motif:HMM:NF016258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990063 - Bam protein complex [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HIR6482294.1"
                     /translation="MVRLLASTLVLALLLAAGCSFRAPKFSLPRVHKITVQQGNVITQ
                     DMIDQLKPGMTRSQVAYVMGEPVFRNSFDESRWDYVYTVEVPGIFQDERRVSLYFEND
                     LLAYFTGDYAPSAAAGAAGAATDTSTAAEQG"
     gene            complement(3773..4042)
                     /locus_tag="AC16PP_11485"
     CDS             complement(3773..4042)
                     /locus_tag="AC16PP_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HIR6482295.1"
                     /translation="MKVDVVYALPDRQTQVGVSLADGATVADALGAVADTEPFSALDL
                     AAVPVGVFGESVGRDRQLRDGDRVEIYRPVLVDPKEARRRRAASA"
     gene            complement(4039..5400)
                     /locus_tag="AC16PP_11490"
     CDS             complement(4039..5400)
                     /locus_tag="AC16PP_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR6482296.1"
                     /translation="MAPATESRHGMWSSRWLFVLAAAGSAVGLGNIWKFPYIAGENGG
                     GAFVIVYLVCIALVGVPIMMAEVMMGREGRQSPINTMRTLTEAHGRSPLWVLIGWMGV
                     LAGFLILSYYVVIAGWALHYIWKMADGTFAGATADVANQTFNDFLADPWALMFWQTVF
                     MAATVWIIARGVIKGLESAIRWFMPLLFVLLMVLLGYAVTSDGFSQGWDFMFDFNWSA
                     LDANAVLIAMGQAFFTLSLGMGAIMAYGAYLPSGTSITTTVATIAGLDTLVALAAGLA
                     IFPIVFANGLEPGQGPGLMFVTIPLAFGQMPLGLVFGTVFFVLVSFAAITSAISISEP
                     ALAYLVEQYNAKRSRVAITLGVIGWLLGVGTVLSFNHWADVHVVGELTYFEFVDYASQ
                     NVLLPLGGLFIALFAAYALPRSVIGEQLGITDGWRALTWKFFCGLVAPLGVLAVFLYN
                     FLA"
     gene            5495..5971
                     /gene="smpB"
                     /locus_tag="AC16PP_11495"
     CDS             5495..5971
                     /gene="smpB"
                     /locus_tag="AC16PP_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR6482297.1"
                     /translation="MNSKTGKPAPKTIALNKRAKRDYHFEQTFEAGLVLEGWEVKSLR
                     AGKAQLVDSYVLVRDGEAWLLGANISPLPSASTHVVADPQRTRKLLLHTKEIAQIFTA
                     TRQKGYACIATALYWKGNRVKCELALGKGKKLHDKRATERERDWNRQKQRIMKQAG"
     gene            complement(5968..6453)
                     /gene="ribH"
                     /locus_tag="AC16PP_11500"
     CDS             complement(5968..6453)
                     /gene="ribH"
                     /locus_tag="AC16PP_11500"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR6482298.1"
                     /translation="MAQTAIDISQLPMIENAGRVAILKSKWYPELVDSMARCCEGVLR
                     DRGIDRVEVHTLPGSLELPLAAADLLAADSGGDIDAIVCFGIILKGETLHFEMITNEC
                     MRGLGEVMQRYRRPVIVEILPVLEMAHAESRAADDEFNKGIEAAAAAIEMIAWRRAVG
                     A"
     gene            complement(6830..7027)
                     /locus_tag="AC16PP_11505"
     CDS             complement(6830..7027)
                     /locus_tag="AC16PP_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482299.1"
                     /translation="MSTKPRLAPAIPGSEADFASLMAQAPNSTALFFELYAEFWQNGV
                     VAEEIKEMTRMRNARITDCGY"
     gene            complement(7043..7540)
                     /locus_tag="AC16PP_11510"
     CDS             complement(7043..7540)
                     /locus_tag="AC16PP_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6482300.1"
                     /translation="MTWIDEVEGATAMERVLAQRPELLARFKAFYRSFWEDGLVSRRV
                     LELCRLRIAAIHDCAQEWGIRDAEVALTQPQMEALRSGDLSNFDPPERAALELAERVP
                     YQHHQLTDEEVVHAEGLLGGPGTVSLLTALAFFDVVCRLRLTLELDEESALLASPPLA
                     DGAMV"
     gene            7604..8299
                     /locus_tag="AC16PP_11515"
     CDS             7604..8299
                     /locus_tag="AC16PP_11515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR6482301.1"
                     /translation="MKREAGPGAIRSSLEILAILALTPVGLVLGGLSGSAALSSITGV
                     ALSVGAATLFLRASGESWRSIGFGRAMPVKRFVGFTAAALLGSYVATTFLVTPVMKAL
                     GAPPLEIGLLTRLIEGDLVSYLIFLLPIAWGSAAFGEELIARGFILHRFSNLFGDGVA
                     IVAQAALFALAHAYQGPVGMANIFALALVFGMVYVRCGRNLWPVIVAHGLVDTVGITL
                     IYLGRADLLLGAS"
     gene            complement(8312..10003)
                     /locus_tag="AC16PP_11520"
     CDS             complement(8312..10003)
                     /locus_tag="AC16PP_11520"
                     /inference="COORDINATES: protein motif:HMM:NF006203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HIR6482302.1"
                     /translation="MQSESVASRYLSLLAERGISHLFVNAGTDFAPLVEAYARSGDAQ
                     GPALPAPVLCTHENLAVGMAHGAYLVSGRPQAVMFHVSVGTANAVCAVTNAARDNVPL
                     LVTAGRTPILEDGALGARDTHIHWAQEMFDQAGMLRELVKWDYELRDPRQVHQVVDRA
                     LSMATSHPRGPVYLSLPREVLAEQMPDADAGARRTAVPVAVEPESSAIEELAGRIAAA
                     RCPVIVTSASGCEPDSVAVLADLADRFAIGIAEMMPRYLNAPMDHELHLGTQMQSIFD
                     AADLIVFLECDVPWIAARAAPRPETFIVQAGTDPLYSRYPMRTHRSDLTLAATPTALL
                     TGLADALASRAGQVDPDRRARLVELARRGKAAIAKLKAAEAEREGTITRAGLSAVLGD
                     ALDDETVIFNEYWGVPELLGRRQPGTYFYLPPAGGLGWGLPAALGAKQAAPEKTVVAA
                     VGDGAYLFANPAACHHAAAKHGLPVLTVIANNASWGAVDFATRAVYPDGSAVQSGERR
                     LSDLSPAPAFEAYCEASGGFGERVTEPSALAPAVERALKAVRDEGRQALLNVACV"
     gene            10115..11263
                     /locus_tag="AC16PP_11525"
     CDS             10115..11263
                     /locus_tag="AC16PP_11525"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6482303.1"
                     /translation="MHVQPIISEIARQADAADRSRTVDPDVIAAIKRSDVMGLSASAG
                     LGGLDAPVTEIARELGAVAPACSSTAWCLWNHLSTFHLFAGLLGPEHADFLKDIVERH
                     EWVCFPAGASTAVRAEEHDGEFSLRGTAAFGSGARYAEWSGVSFVPAEGGPPEFSMVD
                     LRSEGVRIDPTWEAMSLRASATDHVHYEGVRVPAERRVPFHFRYREVFRNPDHAMIDA
                     RYREDWVALSDLWLGAMAVGVAQACLDECCDGIRERVAILGTKMAERPTIHVNLGQAA
                     AAINAARDTVFAALSETDERLDSSVIPDEADYLRQVSASMQALQLCDESMRLMLRVLG
                     GNGLREGSNFERRYRDFQAMPLHINAHRDRITEQVGRHLLGLETENPF"
     gene            11288..12874
                     /locus_tag="AC16PP_11530"
     CDS             11288..12874
                     /locus_tag="AC16PP_11530"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR6482304.1"
                     /translation="MTSNFPTTVTRRLASLLALAAIFAVLGGCGGGGGGSSPAVAVAP
                     EPEPEPEQWQPGVFEPASNFKDMCASPRIGAQFPDTQGTTTDENNWLRSWSNDLYLWY
                     EEIEDRDPARYTTPDYFDLLKTFASTPTGAPKDRFHFTYPTQEWEALSQSGQQPGYGA
                     AFVLLAAAPPRELAVAYIEPASPAEAAGLVRGARVLEIDGVSVVNGSDVDTLNAGLYP
                     SAAGEEHVFVVQEPGGGSRTVTLTSAIVTEDPVQFVKVIDSLTGPVGYLHFTRHIATA
                     ERELIDAIEFLGNAGVTDLILDVRYNGGGFLDIANELAFMIAGGGAASGRVFAEIQFN
                     DKHQTVNPVTGAVLEPEYFHTTTQGFSAEAGDPLPTLNLDRLYVLTTADTCSASESIV
                     NGLRGIDLDVVQVGSPTCGKPYGFYATDNCGTTYFSIQFKAVNAKGFGDYPDGFAPED
                     GAPAGAVTLPGCGVDDDFDNPLGDPAEALLATALEHRAGLGCPVGTATAARAEPRLAR
                     KGSGGEPLTPPRPYGMVLAR"
     gene            12876..13268
                     /locus_tag="AC16PP_11535"
     CDS             12876..13268
                     /locus_tag="AC16PP_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482305.1"
                     /translation="MRKICLIPLVLASACQSTTAEQLPAVLIGDRLEAIAEITEVIAD
                     ALRTEVSLSPSAFSESSLLTLEHAVGSDARGSAATGLLLERPEQFRLLWSDDGCSLTR
                     LRTGETLPLDAAGCRPEAEATAGRAQSP"
BASE COUNT         2338 a         4589 c         4412 g         2187 t
ORIGIN      
        1 ggatgacgcc cgccttctcc agggtggtga ggaagagctt gagagacagc tcgacgccct
       61 ccttgatcgc cccggccgcc tcccccgcag actcccccga ggcctcgacg gccttctcca
      121 ggctgtccag cacgggaagc agatcgctcg tgaaccgctc gagggcgtac ttgtgcgcgt
      181 tttccacatc ccgcgccgcc cggcgcctga cgttctccat ctcggcctgg gctcgcagca
      241 cctgatcctt gagtccggca acctgccgct cggcctccgc aagcggatca tcgatctccg
      301 cctcggacgc gctaggcggg ggggcctcct gttcgagcgg gtccgattcg accacttcgg
      361 tgtgcgactc ctcgctgccg ccgtccaaat cggctgcgtc cggtttgtcg tccgtcatgc
      421 aaagcactcc ttcgagccag ctttgtggaa actatcagcc tcgcggctga cgtcgcagat
      481 gtgggggcgg cggcgatcta tttcaagccc gggcgctcac ccgtagttca tggcggcgcc
      541 gagaacccga gcggtgacgt ccaccacggg aatgacatcc tggtaggcca tacgggttgg
      601 gcccaccaca ccgagcaccc cggcgagctc gccgttcacc tcgtacgggg cggtgaccag
      661 gctcatgtcg cccaggatgt ggtaacccga ctcctcaccg atgaagagct gtatcccgtc
      721 tgaatccagg cacctgtcca gcaggtgcag aatgctccct ttgcgggaga aggcgtcaaa
      781 cagcgtgcgc accgtcgcca tgtctccgct gacgtcgagc agattgctct ctccggcaat
      841 caccagctcc ttgcccaacg gctggtcgga ttccgagaac acgcgaccgg ccatgtccag
      901 agccgtctgc atgagcctgt ccatgccgtc cttgtcggcc tgcatgctgg aaagcagggc
      961 gtcgcggata ctcaccagcg acaggccgcc gaactcccga ttgatgaagt tggccgcctg
     1021 ggcgagctcc gtgtcctcgt attcccggtc cgtgtggatt acccggttct gaacctcgcg
     1081 gtcgttgaga acgaggatga ccagaacccg gtcaccagac aggcgcagga actcgacgtg
     1141 cctgagggca acctgatcgc ggcggggcag cgtcaccacg caggtcatgc gggtaaggtg
     1201 ggagagcagg ttcgacgccg actcgaccag ctcctgggga gaaagatccg gattgagttc
     1261 cagctcgagc cggtggatct gctcctcgcc caaaggctgc acggccagca ggctgtccac
     1321 gaaaaagcgc aaccccagat tcgtgggcac cttccccgcc gacgtgtgtg gcgaccggac
     1381 cagaccgcga tcctctagct cggccatgat gttgcgcacc gtagccgggc tcacctccac
     1441 ctcggggaag gcggcgagct tcttcgaggc aaccgggctg ccgtccgcca gatagcgctc
     1501 cacgagcagc ttgaagagaa gctgcgcacg gtcgtctacg ttgtcttcta cttgctttct
     1561 ggccattttc gttggctaat gattacggtg cggcgctctg aaggcccacg gtacaggcgt
     1621 tctgacagtc cactatgaac ctaattcatc ccgcgtcaag ccatgcttag agagctcttc
     1681 gtaagggatt ttgctctggt ggagtcgctg gagatcggtc tttgcgaggg gctgaccgtg
     1741 atcaccggcg agtcgggagc gggcaagtcc atactgctcg gcgccctggg gctggttcta
     1801 ggcgatcggg cagacacgga caccatcagg cccggcgcca agcgggcgga tgtcaccgcc
     1861 gagttcgacc tcagcggcca gcccgccgct caggcgtttc ttgccgagca cgacctggag
     1921 gacgccgacc atcccgaaag atgcctggtc cgtcgggtgg tcaaccagga gggtcgctcc
     1981 agagccttca tcaacggcgc cgcggtaacc cgcgctctgc tgcgcgagtt cgccgaggac
     2041 ctcgtggaca ttcacggtca gcacgagcac cagcgcctgg ccgagccggc cacccagctc
     2101 aagctcttgg acgattacgg cgtggacgcc gcgctcctgg agtcgtgccg ggagagctat
     2161 cgcttctggc agcgggcagt ggcagaagcg gacgccctcg aggccgccct ggcaagcaag
     2221 caggaccgcg cctcactgct ctcttaccag ctcgaggagc tggccgcgct ggggctagag
     2281 ccaggggagt tcgaaaccct ggaaagcgag cacaaaaggc tctcccaggc ccaggacctg
     2341 cgcgagaccg tcggccacgg cctcgcggtg ttggaggagg ccgacgtcct gggccgcgtc
     2401 tcgggcgcgc tggccaacct ggatgacgag catccggctc ttcaggctgc acgggatctg
     2461 ctgtcatccg ccgacagcct cgtgaccgac gcggtacgcg agctgcgccg atacgacgat
     2521 tccctggacg tggatgaggg ccagctcggg ctgctggaag agcggctgac gagcgttgtc
     2581 gacctcgccc gcaagcatcg ggttcagccg gaccggctgg tcgagcacat ggccttactg
     2641 caggccgagc tcgacgcgat ctccaccgac cgcggctccc tcgacgccct gcgcgaggcg
     2701 gccgccggac acgaagccga gttccgctca accgccggcg acgtgtctct ccagcggcac
     2761 gaggcggccg ttcactttgc ctcggccgtc agcggttgca tgcagaatct gggtatcggc
     2821 ggcggcgagc tgcgcctggc cttcaaagag ggcgagtccg agcgtgggct ggagagcgtg
     2881 gagtatcagg tgatgaccaa ccccaaatac ccggaggccc cgctcgcccg gatcgcctcc
     2941 ggcggcgagc gtgcccgcat cagcctcgcc atccaggtgg tggccgctga gaagtcggcc
     3001 ctgccgtgcc tggtcctgga cgaggcggac gtgggcatcg gcggaaccac ggcggacgtg
     3061 ctcggaaggc tcttgagatc tctggcacgg cacactcagg tcatctgcgt cacccacgcc
     3121 ccccaggtcg ccgccctggg caatcatcac ctgcgggtga ccaaatccag cgatcaggac
     3181 acgcgcatcg agcccctttc cgaggacacc cgaatcgacg aggtcgcgcg gatgctggcg
     3241 ggttctggcg tcaccgacaa gtcgcgggac tatgcccgca ccctgctcga cgaagcccat
     3301 caggcccttc attagcccgc gagatctggg taacatccct ggccttcgcg caaacaccct
     3361 gaggccatgg tccgactgct cgcttccaca ctcgtcctcg cgctgctcct tgcagccggc
     3421 tgcagcttcc gagcgcccaa gttcagcctg cctcgcgtgc acaagatcac cgtccagcag
     3481 ggaaacgtaa tcactcagga catgatcgac cagctaaagc cgggcatgac ccgcagccag
     3541 gtcgcctacg tcatggggga gccggtcttc cgcaactcgt tcgacgagag ccgctgggac
     3601 tacgtctaca cggtggaagt gcccggaata ttccaggatg agcggcgggt gagcctgtac
     3661 ttcgaaaacg atctgttggc ctacttcacc ggcgactacg cgccgtccgc cgccgccggc
     3721 gcggccggag ccgcgaccga cacctccacg gcagccgagc agggatgacc attcaggcgg
     3781 aggctgcccg ccgccgcctg gcttccttgg gatcaaccag cacaggccgg tagatctcaa
     3841 cgcggtcccc gtcccgcagc tgcctgtccc ggccaacgct ctccccgaat acccccacgg
     3901 gaaccgcggc cagatccagc gccgagaaag gctcggtatc agcgacggcc ccaagggcgt
     3961 ctgctaccgt ggcgccatcc gccaggctaa cgcccacctg cgtctgccgg tccggcagcg
     4021 cgtagaccac gtcgaccttc atgcgaggaa gttgtagaga aatacggcca gcacccccag
     4081 cggcgccacc aggccgcaga agaacttcca cgtgagcgcg cgccagccgt ccgtgatgcc
     4141 gagctgctcg ccgatgacgg acctgggcag ggcataggcg gcaaacaggg caatgaacag
     4201 cccgccgagc ggcagcagga cgttctgcga tgcgtagtcg acgaactcga agtaggtcag
     4261 ctcgccgacc acatggacgt ctgcccaatg gttgaaggag agcaccgtgc ccacgcccag
     4321 cagccagcct atgacgccca gcgtgatcgc cacgcgggac cgcttggcgt tgtactgctc
     4381 cacgaggtag gcaagggcgg gctcgctgat ggagatggcg gaggtgatgg cggcaaagct
     4441 caccagaacg aagaacacgg taccgaagac caggcccagc ggcatctggc caaaggccag
     4501 cgggatggtg acgaacatga gccctgggcc ctggccgggc tccaggccgt tggcgaacac
     4561 gatgggaaag atggccagcc cggccgccag ggcgaccagg gtgtccaggc ccgcgatggt
     4621 cgccacggtg gtggtgatgg acgtgccgct cggcaggtac gccccgtagg ccatgatggc
     4681 gcccatcccg aggctcaagg tgaagaaggc ctggcccatg gcgatcagca ccgcgttggc
     4741 gtccagggcg gaccagttga agtcgaacat gaagtcccag ccctgggaga acccgtcgga
     4801 ggtcaccgcg taaccgagca gcaccatcag caggacgaac agcagcggca tgaaccaccg
     4861 tatggccgac tcgagacctt tgatcacgcc gcgggcaatg atccagaccg tggccgccat
     4921 gaagaccgtc tgccagaaca tgagcgccca gggatcggcc aggaagtcgt tgaatgtctg
     4981 gttggcaacg tcggcggtgg ctccggcgaa cgttccgtcg gccatcttcc agatgtagtg
     5041 cagggcccat ccggcgatga ccacgtagta gctgagaatg agaaacccgg ccagcacgcc
     5101 catccagccg atgagcaccc agagcggcga gcggccatgg gcttcggtga gcgtgcgcat
     5161 ggtgttgatt gggctctggc ggccttccct gcccatcatc acctcggcca tcatgatggg
     5221 cacgccgacg agggcgatgc agacgaggta gacgatgacg aacgcgccgc cgccgttctc
     5281 cccagctatg taagggaact tccagatgtt cccgaggccc acggccgatc ccgctgctgc
     5341 cagcacgaac aaccatctgc tggaccacat gccgtgccgg gattcggtag ccggggccat
     5401 gagcacttcc aaggcgatcg cgttgggccg gcattgtggc ccaacgtctt tggggcctca
     5461 accgcatata atccgaggcc atgaggtcct cgatttgaac agcaaaacgg gcaagcccgc
     5521 accaaaaacc atcgcgctga acaagcgcgc gaagcgcgac taccacttcg agcagacctt
     5581 cgaagccggc ctggtgctcg aggggtggga ggtgaaaagc ctgcgcgcgg gcaaggcgca
     5641 gctggtggac agctacgttc tcgttcgcga cggcgaggcg tggctcttgg gcgccaacat
     5701 ctcaccgctg cccagcgcat cgacccatgt ggtcgcggat cctcagcgca cccgcaagct
     5761 gctgctccac acgaaagaga tcgcgcagat cttcacggcc acccggcaga agggctacgc
     5821 gtgcatcgcc acagcgctgt actggaaggg caaccgggtc aagtgcgagc tagcgctggg
     5881 caagggcaaa aagctgcatg acaagcgggc caccgagcgc gagcgtgact ggaaccgcca
     5941 gaagcagcgg atcatgaagc aggcgggcta agcgcctacg gcccggcgcc aggcaatcat
     6001 ctcgatggcg gccgcagccg cctcgatccc tttgttgaac tcgtcatcgg cggcccgtga
     6061 ttccgcatgg gccatctcca gtacgggcag gatctccacg atgaccggcc gccggtatcg
     6121 ctgcatgacc tcgccgaggc cgcgcatgca ctcgttggtg atcatctcga aatgcagggt
     6181 ttcgcccttc aggatgatgc cgaaacagac gatcgcgtcg atgtcgccgc ccgagtcggc
     6241 agcaagcagg tcggcggcgg ccaggggcag ctccagggat cccggcagcg tgtgaacctc
     6301 cacccggtcg atgccccggt cgcgaaggac cccctcgcag cagcgcgcca tgctgtccac
     6361 cagctcggga taccacttgg acttgaggat cgccacccgc ccggcgttct ctatcatcgg
     6421 cagctggctg atgtctattg cggtctgcgc catgtcggca agctctgtgt cagcggaggg
     6481 cgaagaatac ccgaatgagc cgacgcgggc ccgttcagga ccccggtgtc ggcacgacgg
     6541 tcaccggcat ctcctcgggc tccagcccga gcatgatgag caccttggac atgccgagaa
     6601 agcagccgac gcccagcgcc atctcgacga tctgcgcatc ggagaagtga gctttgagag
     6661 cggccttggc atccgcatcg agtgggccag aagccccgat gatggcgtcc gtcagcttca
     6721 gggccgccgt ctcggccggc gcgagcgagc gctcgtaccc gtccgcgatc tgctcgacct
     6781 tcgcttcgtc cagccccgcc gcacgggcgg agtcgaatcg tacgtttttt cagtagccgc
     6841 agtcggtgat gcgcgcgttg cgcatgcgcg tcatctcctt gatctcttca gcgacgacgc
     6901 cgttctgcca gaactcggcg tagagctcga aaaaaagggc ggtggagttg ggcgcttgag
     6961 ccatgagcga tgcgaaatcg gcttcactcc cggggattgc gggtgccagt cgaggcttgg
     7021 tggacatcgg atgctcccct gatcagacca tggcgccgtc ggcaaggggc gggctcgcga
     7081 gcaaagcgct ctcctcgtcc agctccagcg tgagcctgag cctgcagacg acgtcgaaga
     7141 aagcgagcgc cgtcagcagg gacaccgtgc ccggcccacc caggagcccc tctgcatgca
     7201 caacctcttc atcagtcagc tgatgatgct gataaggcac gcgctcggcc agttccaatg
     7261 ccgcccgctc aggggggtcg aagttggaga ggtctccgct tctaagcgct tccatctgcg
     7321 gctgcgtcag ggccacctcg gcatcccgga tcccccattc ctgggcgcag tcgtgaatgg
     7381 ccgcgatgcg cagcctgcac agctcgagga cccgccgcga gaccagcccg tcttcccaga
     7441 agctccggta gaaggccttg aaacgggcca gcagctccgg gcgctgtgcc agcacccgct
     7501 ccatggccgt tgccccttca acctcgtcga tccacgtcat gggtgcgcgc ctttccaaat
     7561 gaggcaaaaa tagcacttcc catgtaatcc ttcgaggcgg accatgaagc gtgaagcagg
     7621 gcccggcgcg atccgctcgt ccctcgaaat cctcgccatt ctggcactga cccccgtggg
     7681 tctggtgctg ggagggctgt cggggtccgc ggcgctcagc tccatcaccg gcgtggcgct
     7741 gtccgtcggg gcagccaccc tcttcctgcg cgcgagcggt gagagctggc gcagcatcgg
     7801 cttcggccgc gccatgccgg tcaagcgctt cgtcggtttc accgccgccg cgctacttgg
     7861 cagctacgtg gccaccacct tcctggtgac gccggtcatg aaagccctcg gcgcgccccc
     7921 gctggagatc ggtctgctca cccggctgat cgaaggcgac ctggtgagct atttgatctt
     7981 cctgctgccc atcgcctggg gaagcgccgc gttcggtgag gagctcatcg cgcggggctt
     8041 catccttcac cgcttttcca acctgttcgg cgacggggtc gccatcgtcg cccaggcggc
     8101 cctgttcgcc ctggcgcacg cgtatcaggg gcccgtcggc atggcgaaca tctttgccct
     8161 ggcgctggtg ttcgggatgg tctacgtacg ctgcgggcgc aatctgtggc ccgtgatcgt
     8221 cgctcatggc ctggtcgaca ccgttggtat caccctgatc tacctgggcc gggccgacct
     8281 cctactgggg gcaagctgat ccgggccgcc gtcagacaca ggccacgttc aacagcgcct
     8341 gccgaccttc gtcccggacc gccttcaacg cgcgctccac ggcgggggcg agggccgagg
     8401 gctcggtgac ccgctcgccg aaacccccgg aggcctcgca gtaggcttcg aaggccggtg
     8461 caggggaaag gtctgaaagg cgtctttcgc cactttgcac cgcgctgccg tccgggtaga
     8521 cggcgcgggt agcgaaatcg accgcgcccc agctggcgtt gttggcgatc accgtgagaa
     8581 cggggagacc gtgcttggcc gcggcatggt ggcaggccgc cgggttggcg aagaggtacg
     8641 cgccgtctcc caccgcggcc accaccgtct tctccggcgc cgcctgcttg gcgcccagcg
     8701 cggcgggcaa tccccacccc aggccgccgg cgggcggcag gtaaaagtag gtacccggct
     8761 gccggcgccc gagcagctcc ggaacccccc agtactcgtt gaagatcacg gtttcgtcgt
     8821 cgagcgcgtc gccgagcacg gcggaaagac ctgcccgggt gatggtgcct tcgcgctccg
     8881 cttccgctgc ctttagcttc gcgatggccg ctttgccacg ccgggcgagc tccaccagcc
     8941 gggcgcggcg gtcgggatcg acctgcccgg cgcgcgaggc caaagcatcc gcgagcccag
     9001 taagcagagc cgtaggcgtc gccgccaggg tgaggtcgga gcgatgagtg cgcatggggt
     9061 aacgcgagta gagaggatcg gtcccggcct ggacgatgaa agtttccggc cgcggggccg
     9121 cgcgcgccgc gatccagggc acgtcgcact ccaagaagac gatgagatcc gccgcgtcga
     9181 agatggattg catctgggtg ccgaggtgaa gctcgtggtc catgggggcg tttaagtaac
     9241 ggggcatcat ctcggcgatg ccgatggcaa agcggtcggc cagatcggca agcacggcca
     9301 cgctgtcagg ctcgcagccg gacgccgagg tgacgatcac cggacaacgt gcggccgcga
     9361 tccggccggc cagctcctct atagccgagg attccggctc cacggcaacc ggtaccgccg
     9421 tccttcgagc cccagcatcc gcgtccggca tctgctccgc gagcacctcc cgcggcaacg
     9481 agaggtagac cgggccgcgc gggtgcgagg tcgccatgct caatgccctg tccaccacct
     9541 ggtgaacctg ccggggatcg cgcagctcgt agtcccactt gaccagctcg cgcagcatgc
     9601 cggcctgatc gaacatctcc tgggcccagt gaatgtgggt atctcgcgca cccagcgcac
     9661 cgtcttcgag tatgggcgtc cggccggcgg tcaccagcag cggaacgttg tcgcgcgcgg
     9721 cgttggtgac ggcgcacacc gcgttggcgg tgccgacgct gacgtggaac atcaccgcct
     9781 ggggccgccc gctgacgagg taggccccgt gggccatgcc cacggccagg ttctcgtggg
     9841 tgcagagcac gggtgccggc agcgcagggc cctgggcatc accggagcgg gcgtacgctt
     9901 cgaccagggg cgcgaagtcg gttcccgcgt tgacaaagag gtggctgatc ccacgctcag
     9961 cgaggagcga aagataccgt gatgcgaccg actccgactg catgcccttt ctccgtgtga
    10021 cgatcactcg ccgagcccaa ttcgcggcgg ccgcctaggc tatcacttta ccgcagctac
    10081 gcgccgggcg atcatgcccg gctcgggagg aagcgtgcac gttcaaccca taatttcgga
    10141 aatcgccagg caggctgatg cggccgaccg gtcccgcacc gtggacccgg acgtcatcgc
    10201 ggccatcaag cgttcggacg tcatggggct gagcgcgagc gccggtctcg gcgggctgga
    10261 cgcgcccgtg acggagatcg cccgggaact cggcgcggtg gcgcccgcgt gcagctctac
    10321 cgcgtggtgc ctgtggaatc atctgagcac gtttcatctc ttcgccgggt tgctcggccc
    10381 ggagcatgcg gattttctca aggacatcgt cgagcggcac gagtgggtct gctttcccgc
    10441 cggcgccagc acggccgtcc gggcagagga gcacgacggc gagttcagcc tgcgcggcac
    10501 cgccgcgttc ggctccggcg cccgctatgc ggagtggagc ggcgtgagct tcgtacccgc
    10561 cgagggcggg ccgcccgagt tcagcatggt cgacctgcgc agcgagggcg tgcgcatcga
    10621 ccccacctgg gaggcgatga gcctgcgcgc gtccgccacc gaccacgtgc actacgaggg
    10681 ggtgcgcgtg ccggccgagc gccgggtgcc cttccacttc cgttaccggg aagttttccg
    10741 caatcccgac cacgccatga tcgacgcgcg ctaccgggag gactgggtag cgctgtctga
    10801 cctctggctc ggcgccatgg cggtcggggt ggcccaggcc tgcctcgacg agtgctgcga
    10861 cggcatccgc gagcgggttg ccatactcgg gaccaagatg gccgaacgcc cgaccatcca
    10921 cgtgaatctg ggccaggccg ctgccgccat caacgcggcc cgcgacacgg tgttcgccgc
    10981 cctgtcggaa accgacgaga ggctcgattc cagcgtcatt ccggacgaag cggactatct
    11041 gcgccaggtg agcgcttcca tgcaggccct gcagctgtgc gacgagtcca tgcggctcat
    11101 gctgcgcgtc ctgggcggca acggcctccg ggagggttcg aacttcgagc ggcgctaccg
    11161 cgacttccag gccatgcccc tgcacatcaa cgcacacagg gaccgcatca ccgagcaggt
    11221 cggcaggcac ctgctggggc tcgaaaccga gaaccccttc tgaatcgaag ggtccaacct
    11281 ctgcgtcatg acgagcaatt ttccgacgac ggttacccgg cgactggcct cgctcctggc
    11341 cctcgccgcg atcttcgccg ttctgggcgg gtgcgggggc gggggcggtg gctcctcgcc
    11401 cgccgtcgcg gtagccccag agcccgagcc cgagccggaa cagtggcagc cgggcgtctt
    11461 cgagccggcg tccaacttca aggacatgtg cgcatcgccg cgaatcggcg cgcagtttcc
    11521 cgacacccag gggacgacga cggacgagaa caactggctg cgctcctgga gcaacgatct
    11581 gtatctgtgg tacgaggaga tcgaggaccg ggaccccgcg cggtacacga cccccgacta
    11641 cttcgacctg ctgaagacgt ttgcctccac accgacgggc gcgcccaagg accgcttcca
    11701 cttcacgtac cccacccagg agtgggaagc cctgagccag tccggccagc aaccgggcta
    11761 cggggctgcc ttcgttctcc tggccgcagc gccgccccgg gagctggcgg tggcgtacat
    11821 cgagcccgcc agccccgcgg aagcggcagg cctcgtccgg ggagcccggg tactggagat
    11881 cgacggcgtc tccgtggtca acggatcgga cgtggacacg ctgaacgcgg ggctttaccc
    11941 gtcggccgcc ggcgaggagc acgtcttcgt cgtgcaggaa cccggcggcg gctcgcgcac
    12001 ggtcaccctg acttccgcca tcgtcaccga ggacccggtc cagttcgtca aggtgatcga
    12061 ctctctgacc ggtcccgtgg gctatctgca cttcacccgc cacatcgcca ccgccgagcg
    12121 agagctgatc gatgcaatcg aatttctggg gaacgccggc gtcaccgatc tgatcctgga
    12181 cgtccgctac aacggcggcg gtttcctgga catcgccaac gagctggcct tcatgatcgc
    12241 cggcggcggc gcggccagcg gccgagtgtt tgccgagatc cagttcaacg acaagcacca
    12301 gacggtcaac cccgtcaccg gcgcggtcct cgagcctgag tactttcaca ccaccaccca
    12361 gggcttctcc gccgaagccg gcgaccccct gcccacgttg aacctggacc ggctctacgt
    12421 gctcaccacc gccgacacct gctcggcctc cgagtccatc gtcaacgggc tgcgcggcat
    12481 cgacctcgac gtggtgcagg tcggatcgcc cacctgcggc aagccgtacg gcttctacgc
    12541 cacggacaac tgcgggacga cgtatttttc cattcagttc aaagccgtca acgcgaaggg
    12601 gttcggcgac tacccggacg gcttcgcccc ggaagatggc gcccctgccg gcgccgtgac
    12661 gctgcccggg tgcggcgtcg acgacgactt cgacaacccg ctcggagatc cggccgaggc
    12721 cctgctggcc acggcgctgg agcaccgcgc aggcctgggc tgcccggtgg gcacggccac
    12781 agccgccagg gcggagccgc gtctggcccg caaaggctcg ggtggcgagc cgctcacgcc
    12841 gccaaggcca tacggcatgg tgctggcacg ttgagatgag aaaaatctgc ctgatcccgc
    12901 tcgtgttggc gtccgcttgt cagagcacca ctgcggagca gctcccggcg gttctgatcg
    12961 gcgaccgcct cgaggccatc gcggagatca ccgaggtgat cgccgacgcg ctgcgcacgg
    13021 aagtttcgct gtctccgtcg gctttctcag aatcgagcct tttgacgctg gagcacgccg
    13081 tgggctccga tgcccgggga agtgctgcca cgggattgct ccttgagcgg cccgagcagt
    13141 ttcggctgct gtggtcggac gacggctgct cccttacgcg cctgcgcacc ggagagaccc
    13201 tgccgctgga tgccgccggc tgccgtcccg aggcggaggc gacagcgggc cgtgcacaat
    13261 ccccttagct ttcaatcagg attcggccag cggcggcaag ccgtggcgtc agcgtcctgc
    13321 gccggcgtgc tttctgtact agactgggtt tgcccgccgg gacttgcaaa cctcctggct
    13381 gccagcatcg acaccttatc agggacaagc gggatgaatg agctgccgaa ggaagcgacg
    13441 gatcagacgg atcggggcgt tcgcgacctg atcacggcgg tgtcacgcgt aagcgcttgg
    13501 cacgatagcg ccatcgagta cgagaa
//
LOCUS       DBNHKV010000360         2051 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_547301, whole genome shotgun sequence.
ACCESSION   DBNHKV010000360 DBNHKV010000000
VERSION     DBNHKV010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_547301"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..995
                     /locus_tag="AC16PP_11540"
     CDS             96..995
                     /locus_tag="AC16PP_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HIR6482306.1"
                     /translation="MTRYQIVHKTHYEYARPVSSSYQHLHLTPRSFAKQNVISSDLHI
                     EPAPTSNQTRQDYFGNPVTDVVIRSRHDSLEITSRALLDVNADENILLDLSPPWEAVA
                     GAVGDPRSSEAWDAARFCFPSPHVDPREALAYAEPSLSPGTPFLRSVEHITARIYEEF
                     TYKGGVTDVQTPVEEVLQSRAGVCQDFAHLAIASVRAFGLPARYVSGYLLTRPPQGQP
                     RLTGADASHAWISVWCPEFGWIDFDPTNNLRPRDEHITLGWGRDYSDVSPTRGFIHGG
                     GSQQLEVSVDVTPVPLADHRARR"
     gene            1005..>2051
                     /locus_tag="AC16PP_11545"
     CDS             1005..>2051
                     /locus_tag="AC16PP_11545"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HIR6482307.1"
                     /translation="MSQPNIAQTRTGAVEGRMKDGTLLFSGIPYAAAPEGSLRFRPAA
                     PVTPWTGVRPALKFGPAAPQVATGGLTNSAAVRWSEDCLSLNVCTPALDGALRPVLVW
                     IHGGSFRTGQGAVPWYNGNRFALHGDMVVVSINYRLGALGFSELSRLGSGFETSGVNG
                     LLDQIRALEWVRDNIQQFGGDPDRVTIAGESAGAFSVCSLLGSPLAQGLFHRAIAQSG
                     AAHHTLRKATAERITDLFLESLGIDDPTDLDSLPVERILDAQTEIVQRLEDRSGALAV
                     LGELHDPVGPFYPCVGNAVLPQSPIEAIRSGSGASIPLLIGTNADEATLWGYGDVPEQ
                     RLHEVVQAYNASAPL"
BASE COUNT          375 a          699 c          619 g          358 t
ORIGIN      
        1 acaagccatt gaaaccggta ttttcaggcc aaaaacgggc aaaaatagct atcaaaacag
       61 ggggttattg aggtggccaa aaaaggcagt gtgagatgac gcgttatcag atagttcaca
      121 agacgcacta cgagtacgct cgtccggtga gctcgtccta ccagcatctg cacctcacgc
      181 cgcgcagctt tgccaagcag aacgtgatca gctcggacct gcacatagag ccggccccca
      241 cttcgaacca gacgcggcag gattacttcg gcaacccggt caccgacgtc gtcatccgca
      301 gcagacacga ctcgctcgag atcaccagtc gtgcgctgct tgacgtgaat gcagatgaga
      361 acatccttct ggatctgagc ccgccgtggg aagccgtcgc cggggcggtc ggcgatccca
      421 gatcgagcga ggcctgggat gccgcccgct tctgctttcc ctcgcctcat gtcgatccgc
      481 gggaagctct tgcctacgcc gagcccagcc tgtcgcccgg cacgccattt ctgcgatccg
      541 tcgagcacat cacggctcgg atctacgagg agttcaccta caagggcggc gtcaccgacg
      601 tccagacgcc ggtggaagag gtgctgcagt cgagggccgg ggtgtgccag gactttgctc
      661 acctggccat cgccagcgtg cgggcgttcg ggctgccggc caggtacgtc agcggctacc
      721 tgcttacccg acctccccaa ggacagcctc ggctcaccgg cgcggatgcg tcccacgcct
      781 ggatatcggt gtggtgcccg gagttcggct ggatcgactt cgatccgacc aacaatctcc
      841 gcccaaggga cgagcacatc acgctgggct ggggccgcga ctacagcgac gtcagcccca
      901 cccggggatt cattcacggc ggtggaagcc agcagctgga agtttccgtg gacgtgacac
      961 ccgtcccgct ggccgatcac cgcgcccgcc ggtaacctct tcccatgagc caaccaaaca
     1021 ttgcccagac ccgaaccggt gcagtagaag gccgcatgaa agacggcacg ctcctgtttt
     1081 ccggaatacc ctacgccgct gcaccggagg gttcccttcg tttcaggcct gcggcaccgg
     1141 taacgccctg gaccggagtc cgcccggcgt tgaagttcgg acccgccgct cctcaggtgg
     1201 cgaccggcgg tctcaccaac agcgccgctg ttcgctggtc cgaggattgc ctatccctca
     1261 acgtctgcac cccggcgctg gacggagcgc tgcgcccggt gctcgtgtgg attcacggcg
     1321 gcagcttccg caccggtcag ggagcggtgc cctggtacaa cggcaaccgg ttcgcgcttc
     1381 acggcgacat ggtcgtcgtc agcatcaact accggctggg cgcgctggga ttctccgagc
     1441 tgtcccgcct cggatcgggt ttcgagacct caggggtgaa cggcctcctc gaccagatcc
     1501 gagctctcga atgggtacgg gacaacatcc agcaattcgg cggcgaccct gaccgggtca
     1561 cgatagccgg tgagtctgcg ggcgctttca gcgtctgctc gctgctcggc tcacctctcg
     1621 cccagggact gtttcaccgg gcgattgcgc aaagcggggc cgcccaccac accctgcgca
     1681 aggcaactgc cgagcggatc accgacctct ttctcgaatc gctgggcatc gacgacccga
     1741 cagatctgga ctcgctgccg gtggagcgca tcctcgatgc ccagaccgag atcgtgcagc
     1801 ggctcgaaga tcgctccggc gctctcgccg ttctggggga actccacgat cccgtgggac
     1861 ccttctaccc ttgcgtgggt aacgccgttc tgccacagag ccccatcgag gccatccgca
     1921 gcggctccgg cgccagcatt ccgctgctca ttggaaccaa tgccgacgag gctacgctgt
     1981 ggggttacgg agacgttccc gagcaaaggc ttcacgaggt cgtccaggcc tacaacgcct
     2041 cggcgccgct g
//
LOCUS       DBNHKV010000361         4410 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1256080, whole genome shotgun sequence.
ACCESSION   DBNHKV010000361 DBNHKV010000000
VERSION     DBNHKV010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1256080"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="AC16PP_11550"
     CDS             complement(<1..476)
                     /locus_tag="AC16PP_11550"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR6482308.1"
                     /translation="MSAAPRLDALNTLGVAATADRLVVVESERALQGLLREAADQRGG
                     LVVLGGGSNVVLKRHVPGTVAVIRTRGTHIVPGRGGRVNVSVAAGESWHGLVRFTIGQ
                     GLYGLENLALIPGSVGAAPLQNIGAYGVELADRLISLRAMEVASGAVREFHREECG"
     gene            complement(473..1081)
                     /locus_tag="AC16PP_11555"
     CDS             complement(473..1081)
                     /locus_tag="AC16PP_11555"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF024922.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB
                     family protein"
                     /protein_id="HIR6482309.1"
                     /translation="MTATLPFRSTVLPSTNERASIPQAGYRHFPSYLADTPADVLLGW
                     LRESVDWRSERIRILGKSVAVPRLVAWYGDPGVCYRYSGVEHVAGGWPSQLCSVRDLL
                     RREWSAGFNFVLINRYRDGSDSMGWHRDDEPELAGPIASLSLGATRRLRVRVEGGTGS
                     IALDLSHGALLCHERHLPHALMKTRRPVGERINLSFRTVVSR"
     gene            1053..1823
                     /locus_tag="AC16PP_11560"
     CDS             1053..1823
                     /locus_tag="AC16PP_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6482310.1"
                     /translation="MDLNGKVAVITGGASGLGRATAEMVINNGGKVALFDLNEDLARQ
                     TAAALGDDAAPFAVDVTNDESAAAAVDQVLERFGAIHVNVNCAGIGAAGRTISKEGAM
                     PLKHFNFIIQVNLVGTFNTLRLCAAAMNENEPTTEDGEKGVIINTASVAAFDGQIGQA
                     AYSASKAGVAGMTLPIARDLARSGIRVCTIAPGIFETPMMAGAPDRVRDPLIEMTQFP
                     KRLGNAAEYALLARQIIENTYLNGETIRLDGGIRMSPR"
     gene            complement(1831..2595)
                     /gene="kdsB"
                     /locus_tag="AC16PP_11565"
     CDS             complement(1831..2595)
                     /gene="kdsB"
                     /locus_tag="AC16PP_11565"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003317470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HIR6482311.1"
                     /translation="MAEPFKVVVPARYGSSRLPGKPLADLHGKPMIVRVAERCAASGA
                     EEVVIATDDVRIVEAVTSAGFEATMTRADHASGSDRVMEVARLRAWDDDAIVINVQGD
                     EPQMPPAVVMQVAQILQADPARPVATLCEPIREVAVLTDPNVVKVVRDRWGRALYFSR
                     APIPYERDEAPGKSGQDRGRPIGLRHIGVYGYRLSALQQFVELPVSDLERLEKLEQLR
                     LLENGIPIFVTEACETVPAGVDTPADLERVRTRSPL"
     gene            complement(2588..3673)
                     /gene="lpxK"
                     /locus_tag="AC16PP_11570"
     CDS             complement(2588..3673)
                     /gene="lpxK"
                     /locus_tag="AC16PP_11570"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - lipid-A 4'-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIR6482312.1"
                     /translation="MAEAPTGSTAPGLTGKLTVGQTLRPLAQAWYTGSRWTVLLAPFA
                     WAFERYAVRRRLRFLTGKRRSWRSPVPVIVVGNITAGGTGKTPMVIWLVEQLRALGYS
                     PGVVSRGYGGKRGRKPLLVTPDSDPAVVGDEAPMIASRARCPVVVAADRARAVESLLQ
                     AANSDVVVSDDGLQHYALARDLEIALIDGHRGVGNGRCLPAGPLREPVSRLESVDWVI
                     SNGLGSGIVARETVMTLIPTAFVRVTPGPGEARQRPEDFVAAHPNVNAVAGVGNPARF
                     ALALRELGLAPVLHAQPDHHPYDGSELVFDNDWPVVCTEKDAVKIRRLTDLPADVWYL
                     EVEAHLPDGAAAFLVELLRAHGISRHG"
     gene            complement(3689..4410)
                     /locus_tag="AC16PP_11575"
                     /pseudo
     CDS             complement(3689..4410)
                     /locus_tag="AC16PP_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT          809 a         1501 c         1384 g          716 t
ORIGIN      
        1 aaaccgcact cttcccgatg aaactctcga acggcgcccg aggcgacttc catggcccgc
       61 aagctgatca gccgatcggc cagttccacg ccgtaggcgc caatgttctg caatggggca
      121 gcccctacgg aacctggtat cagggcaagg ttttccagcc cgtacaggcc ctggccaata
      181 gtaaaacgca cgagcccatg ccagctctcc ccggccgcta cggagacgtt cacccgccct
      241 ccccggccag ggacgatgtg cgtaccacgg gtacgaatga cggcgacggt tcccggaacg
      301 tggcgcttga gaacgacgtt gctgccgccg ccgagcacga cgagcccgcc ccgctgatcg
      361 gcggcttcac gcaggagccc ctgcagcgcc cgctcgctct cgaccacgac caaccgatcg
      421 gccgtcgcag cgacgcccag ggtattgagt gcgtccagtc gaggcgccgc gctcaccggg
      481 agacgaccgt gcgaaacgac aggttgatcc gttctcccac gggccggcgt gtcttcatca
      541 gggcatgagg aaggtgccgc tcgtggcaaa gaagcgcccc gtggctcaag tccagcgcaa
      601 tgcttccggt cccgccctcc acccggactc gcagccgacg ggtcgcgccg aggctcagag
      661 acgcgatcgg ccctgccagc tcgggctcgt catcccggtg ccaacccatg gagtcggagc
      721 cgtcgcgata tcgattgatc agcacgaagt tgaagcccgc cgaccattcg cgccgcagga
      781 gatcgcgcac cgagcaaagc tgagagggcc agcccccggc aacgtgttca acgcccgaat
      841 accggtagca gaccccgggg tctccgtacc aggcaacgag ccttggcacc gccactgact
      901 tcccgagtat tctgatccgc tcgcttcgcc agtcgacgct ttcgcgcagc cagccgagca
      961 ggacgtctgc cggggtatca gcgaggtatg acgggaaatg gcgatagcct gcttggggta
     1021 tactcgcgcg ctcgtttgtg gagggaagaa cagtggatct gaacggcaaa gtggctgtca
     1081 tcaccggtgg ggcatccggc ctcgggcgcg cgacggcgga gatggtaatc aacaatggcg
     1141 gcaaagtcgc cttgttcgac ctcaacgagg accttgcccg tcagaccgcc gccgcgctcg
     1201 gcgacgacgc tgcacccttt gccgtcgacg tgacgaacga cgaatccgcc gctgcggccg
     1261 tcgaccaggt tctggaaaga ttcggcgcga ttcacgtgaa cgtgaactgc gcaggcatcg
     1321 gtgctgcggg ccggaccatc tccaaagaag gtgcgatgcc cttgaagcac ttcaatttca
     1381 tcattcaggt gaatctcgtc ggcacgttca acaccctgag gctttgcgcg gccgcgatga
     1441 acgagaacga gccgaccacg gaggacggcg agaaaggcgt catcatcaat accgcctcgg
     1501 tggcagcgtt cgatggccag atcggtcagg cagcgtactc cgcgagcaag gccggcgtcg
     1561 ccggcatgac cttacccatc gccagggacc tggccagatc cggtatccgg gtctgcacca
     1621 tcgcccccgg catcttcgaa acgccgatga tggctggcgc accggaccga gtcagggatc
     1681 cgctcatcga gatgactcag tttccaaaac gcctcggcaa cgcggcggag tacgcattgc
     1741 tcgcccggca gatcatcgag aacacctact tgaacggcga aaccattcgg ctggacggag
     1801 gaatccggat gtcgccccgc taagagcgcc tcacaatggg gatcgggttc ggactctttc
     1861 caggtccgcc ggcgtgtcca cgccggccgg aaccgtttcg caggcctctg tgacgaagat
     1921 cggaatgccg ttttccagca ggcgcagctg ctccagcttc tccaagcgct cgaggtctga
     1981 cacgggcagc tcgacaaact gctgcagggc tgagaggcga tatccgtaga cgccgatgtg
     2041 gcgcaggccg atcggacgcc ctcgatcctg tccggatttc cctggcgctt cgtcacgctc
     2101 gtaaggtatc ggggcgcggg aaaagtacag cgctcgcccc caccggtccc gcaccacctt
     2161 gacgacgttg ggatcggtca agaccgccac ctccctgatc ggctcgcaca gcgtggccac
     2221 cggacgggcc gggtctgcct gaaggatctg cgcgacctgc atgacgacgg cgggtggcat
     2281 ctggggctcg tcaccctgaa cgttgatcac gatggcatcg tcgtcccagg cccgcaagcg
     2341 ggcgacctcc atgacccggt cggaacccga cgcgtgatcg gcgcgggtca tggtcgcttc
     2401 gaaacccgcg gaagtcaccg cttccacgat tcggacatcg tccgtggcaa tcaccacttc
     2461 ctccgcgccg gaggccgcgc agcgttcggc cacccgcacg atcatcggct tgccgtgcag
     2521 atctgccaga ggctttccgg gaagtctgga cgagccgtac cgggcgggca ccacgacctt
     2581 gaacggctca gccatggcgg ctgatcccgt gggctctcaa cagctccact aagaacgcag
     2641 cggcgccgtc gggcagatgc gcctccactt cgaggtacca gacatcggcc ggcaggtcgg
     2701 tcaggcgccg aatcttcacc gcatcctttt cggtgcagac caccggccag tcgttgtcga
     2761 agactagctc gctgccgtcg tagggatgat gatcgggctg ggcatgaaga accggggcaa
     2821 gccccagctc gcgcaacgcc agcgcaaatc tggcgggatt gcccacaccg gcgacggcgt
     2881 tgacgttcgg atgagcggcc acgaaatcct ccggacgctg gcgtgcctct cccggtcccg
     2941 gcgtgactcg aacgaaagcg gtggggatca gcgtcatgac ggtctctcgg gccacgatcc
     3001 cggatcccag gccattgctg atcacccagt cgacgctctc gagccggctg acgggctcgc
     3061 gtagcggccc ggcgggaagg cagcggccat tgccgacgcc tcgatgacca tcgatcagcg
     3121 cgatttcgag atccctcgcc agggcgtagt gctgcagccc gtcgtccgag acgaccacgt
     3181 cgctgtttgc cgcctgcaga agactctcta ccgccctggc acgatcggct gccaccacca
     3241 ccgggcagcg ggcccggctc gcgatcatgg gcgcttcgtc accgacgacg gcggggtccg
     3301 aatccggggt caccagcagg ggcttgcgtc cccgtttacc gccgtaacct ctggagacga
     3361 ctcccggcga gtacccgagc gccctcaact gctcgaccaa ccagataacc atgggcgtct
     3421 tgcccgtacc acccgcggtg atgtttccca ccacgatcac gggaaccggg ctgcgccagg
     3481 agcggcgctt gccggtaaga aagcgcaacc tgcgacgcac cgcgtagcgt tcgaacgccc
     3541 atgcgaaagg cgcgagcagc accgtccagc ggctgcccgt gtaccaggcc tgggccaggg
     3601 ggcgcagggt ctggcccacc gtcagcttgc cggtcaggcc cggagccgtg gacccggtgg
     3661 gtgcctcggc caccgcagcg gaatccggct cgtcgtcgaa ctgggcctga tagagcgcgg
     3721 cgtaatgtcc accccggttc agcagcgcct ggtgagtacc cgcctctacg atcctgcctt
     3781 catcaacgac gagaatctcg tcggcgttct cgatggtgga cagacgatgg gcaatgacga
     3841 gcgtggtgcg tccgcgcatc acctcatcca gcgccgcccg gatatgccgt tcggactccg
     3901 tgtccagagc cgacgttgcc tcgtccagaa tgagcaccgg cgcgtccttg agcagggccc
     3961 tggcgatggc gacccgctgg cgctgacctc ccgaaagcag aacgccgtcg tcgcccacca
     4021 ccgtctgcag tccgtccgga agctcggaga cgaaccgatc cgcatgagcc cgcctcaccg
     4081 cgcgatcgac ctcgtccgga tcagccccgg ccagggctcc gtaggctatg ttgcgctcga
     4141 gggtatcgtt gaagagcgtc acctgctggg tcacaagggc tatctggctg cgcagagctt
     4201 ccaaccggta gcgcggaagc ggcaccccgt ccaggagaat ctcgccccct gtcggctcgt
     4261 aaaaccgggg tatcaggctc gcgatggtgg ttttgccgct gccggagcga cccaccagag
     4321 ccacggtttt tcccggctcg acggtaaagc tgacgtggct gagcaccggt gcgctgccgc
     4381 gttcataggt aaagctgacg tcgcgaaatt
//
LOCUS       DBNHKV010000362         3705 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1821747, whole genome shotgun sequence.
ACCESSION   DBNHKV010000362 DBNHKV010000000
VERSION     DBNHKV010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1821747"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="AC16PP_11580"
     CDS             complement(<1..573)
                     /locus_tag="AC16PP_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR6482313.1"
                     /translation="MRELVRYILVPALVGGAVGLGVMLTSQVNGTPTKPGYAAAVKRA
                     APGVVNIYSSKLVQHPMCEMPRFRDWCERFAGADRGQMQNSLGSGVIVREDGYILTNN
                     HVIEGADEIIVAFSDGQATTATLVASDPETDLAVIRVQASGLTPVPRDPSSVVEVGDV
                     ALAIGNPFGIGQTVSSGIISAKGRAGISRSP"
     gene            684..1784
                     /gene="zapE"
                     /locus_tag="AC16PP_11585"
     CDS             684..1784
                     /gene="zapE"
                     /locus_tag="AC16PP_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HIR6482314.1"
                     /translation="MSPLQKYEEQLTRSGFFDDPAQREVAGLLDDLYRAFMARERLHA
                     SRLRRALRVLGIGAEPEPVRGLYLWGTVGRGKTLLMDLFYECLPGDVRLRMHFHRFMQ
                     RVHHQLTSLAGTPNPLQQVAERFASEASVLCFDEFAVSDIGDAMILAELLAELFRRGV
                     TLVATSNVEPDNLYENGLQRRRFLPAIELIRQHTVIHQVGGDVDYRLRVLERAEIYHV
                     PLDAGAERSLARSFDALAPEAPRDDVDLEIEGRMIRARRVAEDVAWFNFSDLCEGPRS
                     QNDYIELSRIFHAVLISGVPRFNTRSEDAARRFISLVDEFYDHGVKLIVSAEAEPASL
                     YGGERLRFEFERTVSRLLEMQSHDYLARSHRA"
     gene            1867..2295
                     /gene="rplM"
                     /locus_tag="AC16PP_11590"
     CDS             1867..2295
                     /gene="rplM"
                     /locus_tag="AC16PP_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010797309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR6482315.1"
                     /translation="MKTVSARAQDVQRSWLLVDAEDKTLGRLATEVARRLRGKHKPEY
                     TPHVDTGDYVVVINADKVRVTGNKTTDKIYYRHSGYPGGIKGISFEHLQQKHPERIIE
                     KAVKGMLPRNTLGRAMFRKLKVYAGPEHPHSAQQPEPINL"
     gene            2330..2722
                     /gene="rpsI"
                     /locus_tag="AC16PP_11595"
     CDS             2330..2722
                     /gene="rpsI"
                     /locus_tag="AC16PP_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR6482316.1"
                     /translation="MSANQFYGTGRRKTSAARVFISSGTGNIFVNNQSLDSYFGRETS
                     RMIVRQALFVADVADKVDIRVTVRGGGNSGQAGAIRHGIARALVAHDESLRGALRKAG
                     FLTRDAREVERKKVGLRKARKRPQFSKR"
     gene            2853..3503
                     /gene="petA"
                     /locus_tag="AC16PP_11600"
     CDS             2853..3503
                     /gene="petA"
                     /locus_tag="AC16PP_11600"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HIR6482317.1"
                     /translation="MSADKAATDSAAVPKSLDPSKVNKERRYFLIGASAVVGGIGIVG
                     AAVPFVASWQPSAKARALGAPVTVDISKLRPGEILGPTPAWRGNPVFVVYRDEDALRR
                     LEQENDRLADPDSDNAEQQPDYAKNPWRSKRPEIGVYLGLCTHLGCSPKYHGEVKPEP
                     FAADWEGGFFCPCHGSLFDLAGRVVKGVPAPDNLPVPPYQFVSDTVVVIGVDEETA"
     gene            3503..>3705
                     /locus_tag="AC16PP_11605"
     CDS             3503..>3705
                     /locus_tag="AC16PP_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482318.1"
                     /translation="MEQSQPGRIARGWSNFMEWVDERFPLTETFEYHMSRYYAPKNFN
                     VWYLFGVLSMVVLVIQLLTGIWLT"
BASE COUNT          673 a         1156 c         1220 g          656 t
ORIGIN      
        1 ggggctgcgg ctgatgcccg cccggccctt tgcgctgatg atgccgctcg agaccgtctg
       61 gccaatgccg aacgggttgc cgatggccag cgcgacgtcg cccacctcga cgacgctgct
      121 cggatcacgg ggcaccggcg tcaggcccga ggcctgcacg cggatgacgg cgagatcggt
      181 ctcggggtcg ctggcgacca gggtcgccgt ggtggcctga ccgtcagaga acgccacgat
      241 gatctcgtcg gccccctcga tcacatgatt gttggtgaga atgtagccgt cctcccggac
      301 gatgacgccg gatcccagcg agttctgcat ctgcccgcga tcggcccccg cgaagcgctc
      361 gcaccagtct cgaaaccggg gcatctcgca catgggatgc tgtaccagct tggacgagta
      421 gatattgacg acgccgggcg cggcgcgctt taccgcggct gcgtacccgg gcttggtcgg
      481 cgttccgttc acctggctgg tgagcatgac cccgagaccg acggcacccc cgaccagtgc
      541 agggaccagg atgtagcgaa ccagttcgcg cataggcggc ggacgttaac agaaaaactc
      601 ctgccgcgcc ccgcggcggc acgcctcgcc cgtcgggtgg cgccgattgc gccgttcctg
      661 accgagcaag taaagttccg ccgatgtcgc cgttgcagaa atacgaagaa cagctcacgc
      721 gtagcggctt ctttgacgac ccggcgcagc gggaggtcgc cgggctgctg gacgatctct
      781 accgggcgtt catggcccgg gaaaggctgc acgccagccg gctgcggcgc gccctgcgcg
      841 tgctgggcat cggggctgag cccgagcctg tgaggggcct gtatctgtgg ggtacggtcg
      901 gtcgaggtaa gaccctgctc atggacctct tctacgagtg ccttcccggg gacgttcgtc
      961 tgcgcatgca cttccaccgg ttcatgcagc gcgttcacca tcagctgacg agccttgcgg
     1021 gcacgccgaa ccctctgcag caagttgcag agcggtttgc cagcgaggca agcgtcctct
     1081 gcttcgacga gttcgccgtt tcggacatcg gcgacgccat gattctggcg gaactgctgg
     1141 ccgagctgtt caggcgtggc gtgacgctgg tggcgacgtc caacgtggag cccgacaacc
     1201 tttacgagaa cggcctgcag cgccgtcgtt ttctgcccgc catcgagctc atcaggcagc
     1261 acaccgtgat ccatcaggtc gggggagacg tggattatcg cttgagggtt ctggagcggg
     1321 cggagatcta tcacgttccg ctggacgccg gggcggagcg gagccttgcc cggagcttcg
     1381 atgccctggc gccggaagcg ccccgagacg acgtggatct cgagatcgaa ggtcggatga
     1441 tcagggcgcg gcgggttgcc gaggacgtgg cctggttcaa cttttccgat ctctgtgaag
     1501 gtccccgcag ccagaacgac tacatcgagc tctcgcggat cttccacgcc gtgctgatca
     1561 gcggggtgcc tcgcttcaac acccgatcgg aagacgccgc gaggcgtttc atcagcctgg
     1621 tggacgagtt ctatgatcac ggcgtcaagc tgatcgtctc cgctgaggcc gagcccgcct
     1681 cgctctatgg gggagagcgc ctgcggttcg agttcgagcg cacggtgagc cggctgctgg
     1741 agatgcagtc ccacgactat ctggcccgct ctcacagggc ctgagaaggc ccgtcgggct
     1801 ggttgtaaag ggtcaggccc tccgctaaca ttcgcgctcc caaaatcccg gcatcttgaa
     1861 gggctcatga agacggtaag cgcgcgggcg caggacgtgc agcgttcgtg gctattggtg
     1921 gacgcagaag ataagacgct tggtcgcctg gcaaccgagg tggctcgccg tctccgggga
     1981 aagcacaagc cggaatacac tcctcacgtg gacacggggg attacgtcgt ggtcatcaac
     2041 gccgacaagg tgcgggtgac ggggaacaaa accaccgaca agatctacta ccgccacagc
     2101 ggctatcccg gcggcatcaa ggggatcagc ttcgagcatc tgcagcagaa gcacccggag
     2161 cgcatcatcg agaaagccgt caaggggatg ctgcctcgca acacgctcgg ccgggccatg
     2221 ttccgcaagc tcaaggtcta cgccggcccg gaacatccgc acagcgccca gcaacccgaa
     2281 ccgatcaacc tatagccagt tagccgatca ggcccaggac gcacacagca tgtcagcaaa
     2341 ccaattttac ggaacggggc gacgcaagac ttccgcagcc cgcgttttca tctcttcggg
     2401 caccggcaac atcttcgtca acaatcagag cctcgacagc tatttcgggc gcgagacgtc
     2461 ccgcatgatc gttcgccagg cgctctttgt cgccgacgtc gcagacaagg tggacattcg
     2521 cgtgaccgtt cgcggtggag gcaactcggg ccaggctggc gcgatcaggc acggcatcgc
     2581 ccgggcgctc gttgctcacg acgaaagcct tcgaggtgcg ttgcgcaagg ccgggttcct
     2641 gacccgcgac gcgcgcgagg tggagcgcaa gaaggtcggt ctgcgcaagg cgcgtaaacg
     2701 cccgcagttc tccaagcgat agaggcgtcg gcgcgccgtt cgggccccgt ccacaaggac
     2761 ctccgcgcgg gctgcgcgag cctcctgcgg gcaaggatgt gcacccgcgt gtgctagaat
     2821 gcggggctgt ttgaatatgc tgggagcgac ccttgagcgc tgataaagct gcaacggatt
     2881 ctgccgccgt tcctaagagt ctcgaccctt ccaaggtcaa caaggaacgc cgctactttc
     2941 tgatcggagc ctccgccgtg gtaggtggta taggcatcgt gggcgcagcg gtgcccttcg
     3001 tggcgtcgtg gcaacccagc gcgaaagcgc gtgcgctcgg tgctccggtg accgtggata
     3061 tcagcaagct tcggcccggt gaaatactgg gacccacccc ggcatggcgg ggtaacccgg
     3121 tgttcgtggt ctaccgcgac gaggacgcct tgcgcaggct cgagcaggag aacgaccggc
     3181 tcgccgaccc ggattccgac aacgccgagc agcagccaga ttacgcaaag aacccgtggc
     3241 ggtcgaagcg tccggagatc ggcgtctacc tggggctgtg cacgcacttg ggttgctcgc
     3301 cgaaatacca tggtgaggta aagccggaac cctttgccgc tgactgggaa ggcggcttct
     3361 tctgtccgtg tcacggatcc cttttcgatc tggcgggacg ggtcgtcaaa ggggtcccgg
     3421 cgccggacaa cctgccagtg cccccgtatc agttcgtgag cgacacggtg gtggtgatcg
     3481 gcgtggatga ggagacagcg tgatggagca atcgcaaccg ggaaggattg cgcgcggctg
     3541 gtccaacttc atggaatggg tcgacgagag gtttccgctc accgagacct tcgagtacca
     3601 catgtcccgg tactacgcgc cgaagaactt caatgtctgg tatctgttcg gcgttctctc
     3661 gatggtcgtt ctggtgatcc agctgctgac cggcatctgg ctgac
//
LOCUS       DBNHKV010000363         7827 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_617960, whole genome shotgun sequence.
ACCESSION   DBNHKV010000363 DBNHKV010000000
VERSION     DBNHKV010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_617960"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="AC16PP_11610"
     CDS             <1..380
                     /locus_tag="AC16PP_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6482319.1"
                     /translation="FCIDDEAYQATRRVLRSEAWQQDVLARPLEERRGLAAQMREQSR
                     LSNANKAENIMDVSADEVDRTVKEYGVRRLIHGHTHRPGVHPGDWGTRYVLGSWEHCG
                     WLLREQDTVTALECFPLSGRYGT"
     gene            complement(334..972)
                     /locus_tag="AC16PP_11615"
     CDS             complement(334..972)
                     /locus_tag="AC16PP_11615"
                     /EC_number="1.14.99.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 - tRNA
                     2-(methylsulfanyl)-N(6)-isopentenyladenosine(37)
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaE family protein"
                     /protein_id="HIR6482320.1"
                     /translation="MSATRSRAKPEDAAEFLKCSTPVLWFEQAAGNLETLLVDHANCE
                     KKAAGTALSLIYRYVDRPELLQRLSRLAREELRHFEQVHALMCSMGIAYHALAPGRYA
                     ASLMQQVRTHEPARLVDTLLIGAIVEARSCERFAGLVDVLPGGVGELYAALMVSEARH
                     FRGYLDLAQRYGDGSLEERLDHLLGFESELASGPDTQFRFHSGPIAGNIPAQ"
     gene            complement(997..1443)
                     /locus_tag="AC16PP_11620"
     CDS             complement(997..1443)
                     /locus_tag="AC16PP_11620"
                     /inference="COORDINATES: protein motif:HMM:NF018630.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HIR6482321.1"
                     /translation="MTATRRSTPCVGICSTTYGDLVCRGCKRFAHEIVSWNAFDVDQR
                     EAVWSRLEELRTGAIARCLRVLDEERLIAEAEAVRVPQAAQRELLDLAYEALRRWRDR
                     DAPPERLGVEALAGADTAQDLIRQIDREFYSRSLAHYERSFKIAAQ"
     gene            complement(1454..3475)
                     /locus_tag="AC16PP_11625"
     CDS             complement(1454..3475)
                     /locus_tag="AC16PP_11625"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF023418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase TgpA family protein"
                     /protein_id="HIR6482322.1"
                     /translation="MASARASSGVGFAYQIPRNSLALLMFAQVVVVLPFALQLSPWII
                     AVGLFCGYWRAGVYQGRWSYPHRWVKALLVLASVVGVAVSGAGAFSLEAAASLLILAF
                     ALKLIEMKSRRDAYLVIFLAYFVIATQFLFDQSILVAVYELGAICVVTAAMIGLNQLH
                     TRVRPLASLRDASKLVLQALPFTLVLFLLFPRIAPLWTVPLPTAGKTGITDVVKPGAI
                     AALTQSDELAFRVVFEGEIPRYRDLYWRGLVYSEFEDGAWSVGPELPAVPSSLRPEPG
                     RGEVAYEVLLEPTMSDWLFALDTAFPRSAGVASTQDYRLRARDPILSVFRYGVASYPD
                     FVMDERLIGRLITRETRIDLQDSPRAVALARSLHDGDDERFVDAVLELIRQEPFRYTL
                     NPPRLPDAGSIDAFWFESRAGFCTHYAGAFVYLMRSVGIPARMVGGYQGGEINPISNH
                     LMVRQYDAHAWAEVWLRGKGWQRVDPTAAVAPERIERGLSAALSANDRARLSALTSAR
                     LGAWSTLADMLYWVDSLEHRWNLWVIGYDSRFQAGFLERLLGKVTPGRTGLVMLAGGV
                     ASIGLVAVFLFWRRRPRKRHPVERMFLRFAGKVSGYGLQRNPQESPGAFVRRAARNAG
                     WRDEQADQIVAQLNALLYNPAVSWGRAEVASLAAQLRRLQFRLAFASAR"
     gene            complement(3456..4424)
                     /locus_tag="AC16PP_11630"
     CDS             complement(3456..4424)
                     /locus_tag="AC16PP_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6482323.1"
                     /translation="MADTAAHAFVGQRFGRWLDRRIPPGSRITLSQRNVFIFPTPTGF
                     VFGGLLILLVLGAINYQASLVYGVAFLLGSVFLMTILYTFRNLSGLSLELVSTRAGFV
                     GEAVEFSVRVVRPKGAGREGIQLGWPNGIKQWAELYEQEADTLRLYVKGERRGYLNPG
                     RLLVETYYPLGLLRAWTWVDLDARALVYPKPIFQDFPLSEQGRRDEGELIDPLGSEDF
                     TDIKGYQPGDPIKHIIWRSYARSNDVMVKRYASYLEPRLWLDLETVAGPLEERLSRLT
                     GMALTATRLEREFGLRLGGLEIKPATGETHLEQVLRELALHGIRES"
     gene            complement(4430..5404)
                     /locus_tag="AC16PP_11635"
     CDS             complement(4430..5404)
                     /locus_tag="AC16PP_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6482324.1"
                     /translation="MRKSTPIRRSSSYTTTPEAKDLIQALLSALNTVLLGKETQVKLA
                     LGCLFARGHLLIEDLPGMGKTLLSHALAKVLGLSYNRIQFTSDVLPGDIIGTSIFERD
                     TGGFRFIEGPLFAQLVLADEINRATPKSQSALLEAMEERQVTIEGKTRSLPEPFFVIA
                     TQNPTTQTGTFPLPESQLDRFLMRIELGYPEPDAERELLRGGDRRAVLEGLEPVSDLE
                     GLRALQRAVDEVKVSDLLIEYVQRLVAHSRRGSEFAFGLSPRGALALLHGARAWALIN
                     DRSHVVPEDVQAVLPSVVEHRLREAADFSGHGGGALAQKLLSSVDVVG"
     gene            complement(5352..6878)
                     /gene="purF"
                     /locus_tag="AC16PP_11640"
     CDS             complement(5352..6878)
                     /gene="purF"
                     /locus_tag="AC16PP_11640"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR6482325.1"
                     /translation="MCGIVGMVGTQAINQQLYDALTVLQHRGQDAAGMVTSDGNRVHL
                     RKGNGLVRDVFHQQHMQRLVGNVGIGHCRYPTAGSASSAEAQPMYVNSPYGIALAHNG
                     NLTNSESLQADLFREDLRHVNTDSDSEVLLNVFAHELQVQRALQPEPEHIFAAIEGLH
                     RRCRGAYAAIAMIIGVGVVAFRDPCGIRPLVFGRRENGAGFEYMVASESVALDILGFE
                     RIRDVAPGEAVFITNEGDLHTRVCAEQTSLSPCIFEYVYLARPDSIMDEVSVYKCRLR
                     MGEYLAQRILERYAGNDHDIDVVVPIPDTGRTAALPLAYQLNVKYREGFIKNRYIGRT
                     FIMPGQSQRRKSVRQKLNAVDLELRGKNVLLVEDSIVRGTTMQHVIQMARDAGAKKVY
                     MASAAPPIRFPNVYGIDMPTPKEFVAYDRTVEEVREFIGADWLVYQELEDLVASAREG
                     NPTISQFECSVFDGHYVTGDVDAGYLERISLHRSDQMKQRRDAEINADQTVIELHNHA
                     "
     gene            complement(6885..7382)
                     /locus_tag="AC16PP_11645"
     CDS             complement(6885..7382)
                     /locus_tag="AC16PP_11645"
                     /inference="COORDINATES: protein motif:HMM:NF014704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR6482326.1"
                     /translation="MATPDIVILIIILTSAAIGFVRGLLKELLSLASWIAAFILALYF
                     APTVEQWFEGGISEESVRLAIAFILIFAVTLIIGGIIQWLVRKLVETTGLTGTDRFLG
                     LLFGSARGVLVCIIGLIAIRPFAADADWWGESDFSPALLEFEQDVLELMGRAHDVFED
                     VSEGR"
     gene            complement(7382..>7827)
                     /locus_tag="AC16PP_11650"
     CDS             complement(7382..>7827)
                     /locus_tag="AC16PP_11650"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR6482327.1"
                     /translation="AEGVPSIQIEPMAPPKPAPAVTPLGAVAPASDFIEKVDEFRAQV
                     DDEGFLTASGTRFGEPILSEPGAGTSAWAVQLASLADQDNARDLRSQLREQGYEAFIS
                     TVKSGSEVLNRVAVGPILNEIDAEALRAELSQLYDVDARLMAFAN"
BASE COUNT         1497 a         2565 c         2492 g         1273 t
ORIGIN      
        1 ccttctgcat cgatgacgag gcctatcagg ccacgcgccg cgttctgcgc tccgaggcgt
       61 ggcagcagga cgtgctggca cgcccgctgg aggagcggcg cgggcttgct gcccagatgc
      121 gcgagcagag ccgcctgagc aacgccaaca aggcagagaa catcatggac gtctcggctg
      181 acgaggtgga tcggacggtc aaggagtacg gggtcagacg gctaatacat ggtcataccc
      241 atcgccccgg cgttcacccg ggggactggg gaacccgtta cgtgctcggc tcctgggagc
      301 attgcgggtg gctgctgcgc gagcaggaca ccgtcactgc gctggaatgt ttcccgctat
      361 cgggccgcta tggaacctga actgagtgtc cggacctgac gcgagctcgg actcgaagcc
      421 caacagatga tcgagcctct cctccagcga accgtcgccg tacctctgcg ctagatccag
      481 gtatcccctg aaatggcgcg cttccgagac catcagagcc gcgtacagct cgccgacccc
      541 gccaggaagc acgtccacga gcccggcaaa ccgctcacag gatcgggcct ccacgatggc
      601 gccaatcagg agggtgtcaa cgagccgggc cggctcgtgg gtcctgacct gctgcatcag
      661 gctggccgcg tagcggccgg gagccagcgc gtgataggcg atgcccatgg agcacatcag
      721 cgcgtgcacc tgctcgaagt gacgaagctc ctcccgggcg agacgggaca gccgctgcag
      781 cagctcgggc cgatccacgt agcgatagat gagactgagc gcggtgcccg ccgccttctt
      841 ctcgcaattg gcgtggtcga cgagcagagt ctcgaggttc ccggccgcct gctcgaacca
      901 cagcaccggt gtcgagcact tgagaaactc ggcggcatcc tcgggtttcg cgcggcttcg
      961 ggtggcgctc acagcgtggc gctcacagcg tggcgctcat tgagcggcga tcttgaagct
     1021 gcgctcgtag tgggcgagcg accggctgta gaactcacgg tcgatctggc gaatcagatc
     1081 ctgcgccgtg tctgccccgg caagggcctc cacgcccagc cgctccgggg gcgcgtcgcg
     1141 atcgcgccac ctcctgagcg cttcataggc caggtccagc agctctctct gcgcggcctg
     1201 ggggacccgc acggcttcgg cttcggcgat cagccgctcc tcgtcgagga cgcgcaggca
     1261 tcgggcgatg gcgccggttc gcagctcctc gagacggctc cagacggcct cacgctggtc
     1321 cacatcgaag gcgttccagc tgacgatctc gtgggcgaac cgcttgcacc cgcggcagac
     1381 caggtcgccg taggtcgtcg agcatatgcc gacacagggg gtgctgcggc gcgtggccgt
     1441 catgacgatg tcgctacctc gcagatgcaa aggccaatct gaactgaagc cgccggagct
     1501 gcgcggcaag cgacgcgacc tctgcgcggc cccaggagac ggccggattg tacagaagcg
     1561 cattcaattg ggcgacgatc tgatccgcct gctcgtcccg ccatccggca ttgcgggctg
     1621 cccggcggac gaaagcaccc ggcgactctt ggggatttcg ctgcaggccg tagccggaaa
     1681 cttttccggc aaagcgcagg aacatgcgct cgaccgggtg ccgtttgcgg gggcgcctgc
     1741 gccagaagag gaagacggcg acgaggccga tgcttgcgac accgccggca agcatcacca
     1801 gacccgtgcg gcctggcgtg accttgccga gcaggcgctc gagaaatccg gcctggaagc
     1861 gggaatcgta gccaatcacc cagaggttcc agcgatgctc cagcgaatct acccagtaca
     1921 gcatgtccgc cagggtgctc caggcgccga gccttgcgct cgtcagcgcc gacagcctcg
     1981 cgcgatcgtt ggccgatagc gcagccgaga gcccccgctc gatgcgctcc ggggccacgg
     2041 ccgccgtggg atcgacgcgc tgccagcctt tgccgcgcaa ccaaacttcc gcccaggcat
     2101 gagcgtcgta ctgcctcacc atgaggtgat tgctgatcgg gttgatctca ccgccctgat
     2161 agccacccac catgcgcgcc gggatcccaa cggaccgcat gagatagacg aacgcccccg
     2221 cgtagtgcgt gcagaagccg gcgcgagact cgaaccagaa cgcatcgata ctgcccgcgt
     2281 caggcagcct gggagggttc agcgtgtagc gaaagggctc ctggcgaatc agttcgagca
     2341 cggcgtcgac aaagcgctca tcgtcgccgt cgtgcagcga tctggccaac gccaccgctc
     2401 tggggctgtc ctggagatcg atcctcgttt cacgcgtgat caggcggccg atcagccgct
     2461 cgtccatcac gaagtccgga taggacgcca cgccgtatcg gaagacggac aggatcgggt
     2521 cgcgcgcccg cagccgataa tcctgggtcg acgcaacgcc ggccgaacgc ggaaatgcgg
     2581 tgtccagggc gaaaagccag tcgctcatgg tcggctcgag cagaacctcg taggcgactt
     2641 cgcccctccc gggctcgggc ctgagcgagc tcggcactgc gggcagctcg ggcccgacgg
     2701 accaggcgcc gtcctcgaat tccgagtaga cgagcccgcg ccagtacagg tcgcgataac
     2761 gcgggatctc gccttcgaac acgaccctga acgccagctc gtccgactgg gtcagagcgg
     2821 cgatcgcgcc gggcttgacc acgtccgtga tgcccgtctt ccccgcggta ggtagaggca
     2881 ccgtccacag cggcgcgatg cgcgggaaaa ggagaaacag caccagggtg aaaggcagcg
     2941 cctggagcac cagcttggag gcgtcgcgca aagaagccag cgggcgcacg cgcgtgtgca
     3001 gctggttcag gccgatcatg gcggccgtga ccacgcagat ggcccccagc tcgtagacgg
     3061 ccaccaggat ggactgatcg aagagaaact gcgtggcgat cacgaagtaa gcgagaaaga
     3121 tcaccagata ggcgtcgcgg cggctcttca tctcgatgag cttgagagcg aacgccagga
     3181 tgagcagcga agccgccgcc tccaggctga acgcccccgc accgctcacg gccacgccaa
     3241 ccacggacgc cagtaccagc aaggctttca cccatcgatg cggatagctc caccggccct
     3301 ggtagacgcc cgcgcgccag tagccgcaga acaggccaac ggcaatgatc cagggggaga
     3361 gctgcagcgc aaagggcaat accaccacga cctgcgcgaa catcagcagc gccaggctgt
     3421 tgcggggaat ctggtaggcg aagcctacgc cggaactagc tctcgcggat gccatggagg
     3481 gccagctccc tgagcacctg ctcgagatgc gtctcacctg tggcgggctt gatttccaac
     3541 ccccccagcc tcagaccgaa ctcccgctcc aggcgggtgg ccgtcagtgc catgcccgtg
     3601 agacggctca aacgctcttc cagcggcccg gcgacggtct ccagatccag ccagagcctg
     3661 ggctcgagat agctggcata ccgcttcacc atgacgtcgt tcgatcgcgc gtacgagcgc
     3721 cagatgatgt gctttatcgg atctccgggc tggtagccct ttatgtccgt gaagtcctcg
     3781 ctgcccaggg gatcgatgag ctcgccttcg tcccggcgcc cctgctccga aagagggaag
     3841 tcctggaaga tgggcttggg ataaacgagg gcccgggcgt cgaggtctac ccacgtccac
     3901 gcccgaagca atccgagggg gtagtaggtc tcgacgagca gccgcccggg gttgagatat
     3961 cctcgtcgct cacccttcac gtacaaccgc aaggtatcgg cctcctgctc atagagctcg
     4021 gcccactgct tgatgccgtt cggccatccc agctgaatcc cctcgcgccc ggcgcctttt
     4081 ggacgcacca cgcgaacgct gaactccacc gcctcgccga cgaacccggc gcgcgtgctg
     4141 accagctcca ggctgagccc cgacagattg cggaacgtgt agagaatggt catcaggaag
     4201 acgctgccga gcaggaaggc gacgccgtag accaggctcg cctgatagtt gatggcgccg
     4261 aggacaagca ggatgagcag accgccgaaa acgaaccccg tcggggtcgg aaagatgaaa
     4321 acgtttcgct gactgagcgt gatacgcgaa ccaggcggga tgcgtctgtc gagccagcgc
     4381 ccgaaccgtt gcccaacgaa ggcgtgagcg gctgtgtcgg ccacggatgc tagcctacga
     4441 cgtcaacgct cgacagcagc ttctgcgcga gcgcgccgcc cccgtgaccc gagaagtcgg
     4501 cagcttctcg cagccggtgc tccaccacgc tgggcagcac ggcctgcacg tcttccggaa
     4561 ccacatggct gcgatcgttg atgagcgccc aggcccgggc gccgtgcagc agggccaggg
     4621 cgccgcgagg cgaaagcccg aaggcgaact ccgatccacg acggctgtgc gccaccagcc
     4681 gctgcacgta ctcgatcagc agatccgaga ccttcacctc gtccaccgct cgctgcaggg
     4741 cgcgaagccc ttccaggtcc gaaaccggtt cgagcccctc gagcacggcg cgccggtctc
     4801 cccctcgcag aagctccctc tcagcatcgg gttccgggta cccgagctcg atacgcatca
     4861 ggaaccggtc cagctgagac tcgggcagcg ggaaggtgcc cgtctgagtg gtggggttct
     4921 gagtcgcgat cacgaagaac ggttcgggca gcgaccgggt cttaccctcg atggtgacct
     4981 gccgctcctc catggcctct aggagcgcgc tctggctctt gggcgtcgcg cggttgatct
     5041 cgtctgcaag gaccagctgg gcaaagagag gcccctcgat gaaacgaaac ccgccggtgt
     5101 cccgttcgaa gatggacgtt ccgatgatgt cgcccggaag cacgtcactg gtgaactgaa
     5161 tgcggttgta gctcaacccg agcaccttcg cgagcgcgtg ggagagcagc gtcttgccca
     5221 tcccgggcag gtcctcgatg agcagatgac cccgggcaaa gaggcagccg agcgccagct
     5281 tgacctgggt ctctttgccc agaaggacgg tgttgagagc ggacaggagg gcctggatca
     5341 ggtccttggc ctcaggcgtg gttgtgtagc tcgatgaccg tctgatcggc gttgatttcc
     5401 gcatcccggc gctgtttcat ctggtcactt cgatgcaacg atattctctc cagatagccg
     5461 gcgtcgacgt caccggtcac ataatggcca tcgaagaccg agcattcgaa ctgggagatg
     5521 gtcggattgc cctcccgggc gctcgccacc agatcttcca gttcctggta aacgagccag
     5581 tcggcgccga tgaactcgcg gacctcctcg acggtccggt cgtaggcgac gaactcctta
     5641 ggcgtcggca tgtcgatgcc gtaaacgttt ggaaaccgga tgggcggtgc cgcagatgcc
     5701 atgtagacct ttttcgcccc ggcatcccgc gccatctgga tgacgtgctg catggtggtt
     5761 ccgcgaacga tggagtcctc caccagcagc acgtttttgc cacgcagctc gagatccacg
     5821 gcgttcagct tctggcgtac ggatttccgg cgctgactct gacccggcat gatgaacgtc
     5881 cgcccgatgt agcggttctt gatgaaaccc tcccgatact tgacgttaag ctggtaggcg
     5941 agagggagag cggccgtccg ccccgtatcc ggaatcggca cgaccacgtc gatgtcgtga
     6001 tcgttcccgg cataccgctc cagtatccgc tgggcaaggt actcgcccat cctcaggcga
     6061 cacttgtaca cggacacctc atccatgatg gagtccggtc gcgcgagata gacgtactcg
     6121 aagatgcacg gagacagcga cgtctgctcc gcgcagaccc gggtgtgaag atcaccctcg
     6181 ttggtaatga acaccgcctc gccgggcgcg acgtcacgga tccgctcaaa gccgagaatg
     6241 tccagggcca cgctttccga ggcgaccatg tactcgaacc cggccccgtt ctcacgccgg
     6301 ccaaacacca gcggccggat gccgcaaggg tcgcgaaacg caaccacgcc gacgccgatg
     6361 atcatggcga tggcggcgta agcgccccga cagcgccggt ggagaccttc gatggctgcg
     6421 aagatgtgct cgggctccgg ctgaagggct cgctggactt gcagctcgtg agcgaacacg
     6481 ttcaacagaa cctctgaatc gctgtccgtg ttgacgtggc gaaggtcctc gcggaacaga
     6541 tcggcctgca ggctttccga gttggtcagg ttcccgttgt gagcaagggc aatgccataa
     6601 ggcgagttga cgtacatggg ctgcgcttcc gccgagctcg ccgagccagc cgtggggtag
     6661 cggcagtgtc caatgccgac gttgcccacc agacgctgca tgtgctgctg gtgaaagacg
     6721 tcgcgaacga ggccgttacc cttgcgcaga tgcacgcggt tgccatcgct ggtgaccatg
     6781 cctgccgcgt cctgtccacg gtgctgaagc accgtgagcg catcgtagag ctgctggttg
     6841 atggcctggg tgccgaccat tccgactatt ccgcacatgt ctgctcagcg cccctcacta
     6901 acatcttcga atacgtcatg agctctgccc atcagttcga gcacgtcctg ctcgaactcg
     6961 agcagcgcgg gcgagaaatc ggactcaccc caccagtcgg catcggcagc gaacggtcgg
     7021 atcgcgatga gcccgatgat acagaccagc acgccgcgcg cgctgccgaa cagcaaaccg
     7081 aggaagcggt cggtgccggt gagtcccgtc gtctccacca gcttacgcac cagccactga
     7141 atgatcccac cgatgatgag ggtgacggca aagatcagta tgaacgcgat ggccagtcgc
     7201 accgactcct ctgagatgcc gccttcgaac cattgctcga cagtgggggc gaagtacagg
     7261 gcgaggataa aggctgcaat ccaggatgca agcgaaagca gctctttgag gagaccacgg
     7321 acgaacccga tcgcggcgga ggtgagaatg atgatcagaa tgacgatgtc gggagtagcc
     7381 atcagttggc aaacgccatc agccgagcgt ccacatcgta gagctgggac agctccgccc
     7441 gcaacgcctc ggcgtcgatc tcgttgagaa tggggcccac ggccacgcgg ttgagcacct
     7501 cggaaccact cttgacggta gaaatgaaag cttcgtagcc ctgctcgcga agctgcgagc
     7561 gaaggtctct agcgttgtcc tgatcggcaa ggctcgccag ctgcaccgcc caggccgacg
     7621 tgccggcacc cggctccgac aggatcggct ctccgaagcg ggtcccgctg gcggtgagga
     7681 acccttcatc atcgacttga gcacggaact cgtccacctt ctcgatgaag tcgctggccg
     7741 gcgccaccgc ccccagcggc gttactgccg gcgccggctt gggcggcgcc atcggctcga
     7801 tctgaatgct cggcacgccc tcggcat
//
LOCUS       DBNHKV010000364        11657 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1681228, whole genome shotgun sequence.
ACCESSION   DBNHKV010000364 DBNHKV010000000
VERSION     DBNHKV010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11657
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1681228"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..391)
                     /locus_tag="AC16PP_11655"
     CDS             complement(53..391)
                     /locus_tag="AC16PP_11655"
                     /inference="COORDINATES: protein motif:HMM:NF022777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3135 domain-containing protein"
                     /protein_id="HIR6482328.1"
                     /translation="MKSRLPGFDTLVDMARNDPDRLESLRRSLTDAVIAEASCDDTRR
                     RLQGLQFRVDMERRRAATPLAATIRISEMMARSLADLHRSLVAPETLEEDLTRKPPRR
                     SDNVIEFPPG"
     gene            559..1098
                     /locus_tag="AC16PP_11660"
     CDS             559..1098
                     /locus_tag="AC16PP_11660"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003178404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR6482329.1"
                     /translation="MLYQLGERRVCAEGAHFIAPGAMLTGSVTLKANVNVWFNAVIRG
                     DVEPILVGANSNIQDCAVLHTDAGCPLTIGDYVTVGHKAVLHGCDVGDNTLIGINAVV
                     LSGARIGRNCIIGANALVTEGKVIPDNSMVLGSPGKVVRELVDEQIAGLRVSAEGYVE
                     RARWYLAELSEQESVDITS"
     gene            1095..2282
                     /gene="purT"
                     /locus_tag="AC16PP_11665"
     CDS             1095..2282
                     /gene="purT"
                     /locus_tag="AC16PP_11665"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010220253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HIR6482330.1"
                     /translation="MTSIGTPLAGNATRVMMLGAGELGKEVVIELQRLGVEVIAVDRY
                     ANAPGMQVAHRSHVISMLDGDALREVIALEKPDMVVPEIEAIATEVLVELESEGLTVI
                     PTARAARLTMDREGIRRLASETLGVRTSPYRFAATREEYDEAIQEIGVPCVVKPVMSS
                     SGKGQSTVRTAADAAAAWDYAQAGARGGADKVIVEGFVDFDYEITLLTVRHVGGTSFC
                     APIGHRQAGGDYQESWQPQPMAEAALAECKRIGELVTGELGGRGLFGMEFFIAGDQVY
                     FSEVSPRPHDTGMVTLLSQDLSEFALHARAILGLPIPHIQQHGPSASSVVLVEGESRA
                     VRFEGFEAALAEPGTDLRLFGKPEVAGERRMGVALARGRDVDDARKKANAAAAAIRWS
                     FDD"
     gene            2577..4223
                     /gene="leuA"
                     /locus_tag="AC16PP_11670"
     CDS             2577..4223
                     /gene="leuA"
                     /locus_tag="AC16PP_11670"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017700408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR6482331.1"
                     /translation="MPFQKYRAFQAIDLPDRQWPTRTLTTAPRWCSVDLRDGNQALID
                     PMNVAEKLRMFDLLVGVGFAEIEVGFPAASQPDFDFIRRIIDEGRIPDGVAIQVLCQA
                     REELIARTVEALRGAPKVIFHLYNSTSELQRRVVFEMDRSGIIDLAVRGTRLVKAGLE
                     TLDSDITFEYSPESFTGTELDFAVDICTAVAEEWGATPDDPMIINLPSTVEMATPNIY
                     ADQIEWFARNFSRRDSTVISLHTHNDRGTGIAATELALMAGAERVEGTLFGNGERTGN
                     CDIVTMAMNLFSQGVDPELDFRDMPRIRATVEEINKLPVHERHPYAGDLVFTAFSGSH
                     QDAIRKGMAKVDRDHWEVPYLPIDPADVGSSYKETVRVNSQSGKGGVGFLLEEHFGIA
                     LPREMLVEFSAVVQQLTESQGREIKTDEILEALKKEYVVDSGPYRLVDFDLLTGRTSD
                     QRCIARVEVNEQQVTIDGEGSGPIEAFVNGMVETLNEPLNIIDYHEHAMTTGKDADAF
                     CLVAIDDPKQGRCYGVGVSRNTITASFMAIVSALNRRWKA"
     gene            complement(4284..5555)
                     /locus_tag="AC16PP_11675"
     CDS             complement(4284..5555)
                     /locus_tag="AC16PP_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HIR6482332.1"
                     /translation="MSLTELPASVLLIFVFVLTALSAYFSSSETAMIALNRYRLKHLV
                     KQKHRGARKANRLLRRPDRLLGVILIGNNLVNFTAASIATVIGLRFFGDTGVVLAPIV
                     ITVIFLIFAEVAPKTIAAKVPERIAFPSAYLLEPLLRLLYPGVVFINGISNAIVKPFL
                     PKEGEIAADSLSVEELRTVVGEGVGLPGQGQEMLLSILDLEKVSVDDIMVPRSEIVGI
                     DVDADIGDILVQIGNSQHTRLPVFKENINNILGILHLRKVARYLQRKEREEELSKSDL
                     MSLTDEPYFVPEATPLPTQLINFQNEKQRIALVVDEYGEVQGIVTLEDILEEIVGEFT
                     TDFAASIQEIHPQDDGSFFIDGMALLRDINRSLGWDLPTHGPKTLNGLVLEHLETLPD
                     SNLCLRLGEYLIETLHIKENAIKTLKITRVE"
     gene            complement(5555..6325)
                     /locus_tag="AC16PP_11680"
     CDS             complement(5555..6325)
                     /locus_tag="AC16PP_11680"
                     /inference="COORDINATES: protein motif:HMM:NF013725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly family protein"
                     /protein_id="HIR6482333.1"
                     /translation="MLLYLLGTAMQLRRLSRSAPVLLQPLIAVGIPALVLHALTTISV
                     IYTPAGIHLGFFSTGGLVTLVMTAFVMLASLRLPVHNLCVLIFPASALGIIGSLLGES
                     AFVPRQEMPWELLLHVLISITAYSILFMAACQSVLLAIQEHYLRARHGLAFIRLLPPL
                     ETMETLLFSMLWTGLVVLTVAILSGFAFLEDMFAQRVVHHTVLSSLSWLLYATLLAGH
                     KLLGWRGTTAVRWTLIAFLLLVLGYFGSKFVIEILLDA"
     gene            6494..7858
                     /gene="ffh"
                     /locus_tag="AC16PP_11685"
     CDS             6494..7858
                     /gene="ffh"
                     /locus_tag="AC16PP_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR6482334.1"
                     /translation="MFDNLSERLTQTLRGISGKAKLTEENVQDALRDVRMALIEADVA
                     LPVVKDFLSQVQTRALGQTVSSALNPGEEFVRIVHEELVHVMGDDNDALNLATTPPAV
                     VLMAGLQGAGKTTTVAKLALLLKERERKKVAVVSADVYRPAAIEQLATLAGEVGVICV
                     PSSAQEKPLEIARRAVDEARNQFADVLLVDTAGRLAIDADMMAEIKLLHEELQPIETL
                     FVVDAMTGQDAANTAKAFNDALALTGVVLAKADGDARGGAALSVRAITGKPIKFIGMG
                     ERVDALEPFYPDRMASRILGMGDVLSLIEEAERKVDKRKAQKLAKKIQRGRKFDLEDF
                     REQLDQMSNMGGVSSMLEKLPGMGQLPPAAQAQLDDGMFRKMAVIIDSMTPHERRFPD
                     VITGSRKQRVARGSGTQVPDVNRLLKQHKQMQRVMKKMSRKGGMQRMMRGMQGMRPPP
                     GVRR"
     gene            7957..8229
                     /gene="rpsP"
                     /locus_tag="AC16PP_11690"
     CDS             7957..8229
                     /gene="rpsP"
                     /locus_tag="AC16PP_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR6482335.1"
                     /translation="MLTIRLARHGSKKNPFYHITVADRTAKRDGRFVERVGFYNPVAK
                     GQAEELRVDLERIDYWLSVGALASDPVTRLIRRARRQEAAVEATAD"
     gene            8286..8789
                     /gene="rimM"
                     /locus_tag="AC16PP_11695"
     CDS             8286..8789
                     /gene="rimM"
                     /locus_tag="AC16PP_11695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR6482336.1"
                     /translation="MHSQEYVIVGRFGGPYGVHGWIRVTSFTDPPANLLLYGPWHVER
                     DGQWHAYHPVQVRPHQQGLIACYADIPDRSVAAALTGRFIAVPRDCLPVLDAEEYYWR
                     DLEGLEVWNRGARLGVVDHLIDTGANSVLAVRGDSTQTLIPFVSRFVTGVDLAAGRID
                     VDWEEAD"
     gene            8792..9565
                     /gene="trmD"
                     /locus_tag="AC16PP_11700"
     CDS             8792..9565
                     /gene="trmD"
                     /locus_tag="AC16PP_11700"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR6482337.1"
                     /translation="MWCGIVSLFPEMFRRLSELGVLGRAIDRGIVTLELFNPREFARD
                     RHRTVDDRPYGGGPGMVMQVEPVVAAVSAAREAARAALGESVPVIYLSPQGRRLTQQR
                     VSELSQQRGVILLAGRYEGIDERAVSLAVDEEVSIGDYVLSGGELPALVVLDAVTRLL
                     PGTLGNSASIEAESHLDGLLDYPHYTRPENTHGLGVPEVLLSGDHEAVSRWRRKMALL
                     RTWQRRPDLLVGRQLDDEDSALLRECFEELAARTESGEE"
     gene            9572..9922
                     /gene="rplS"
                     /locus_tag="AC16PP_11705"
     CDS             9572..9922
                     /gene="rplS"
                     /locus_tag="AC16PP_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010220244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR6482338.1"
                     /translation="MRRNKVIEELESEQLKSDLPEFGPGDTVVVQVKVREGNRERLQA
                     FEGVVIGKRNRGLNSAFTVRKMSHGVGVERTFQSHSPMIASVSVKRRGDVRQAKLYYL
                     RERSGRSARIKEKV"
     gene            10266..11171
                     /gene="xerD"
                     /locus_tag="AC16PP_11710"
     CDS             10266..11171
                     /gene="xerD"
                     /locus_tag="AC16PP_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIR6482339.1"
                     /translation="MGEVLPKDEAARHTVDEYLDAVWLERGLSENTLAAYRRDLYQLA
                     DMLKRDPLTANDADLLECLALRHRQGYRASSAARWLSSVKGFYRHAAGRGRIGADPSA
                     HLEHPKMGRRLPGSLSEAEVRQLLAAPRLDDPIGLRDKAMLELLYASGLRISELVGLT
                     MSAVNARQGIVRIVGKGGKERLVPMGGEAMRWLERYLHEGRPLIKGSDATAAMFPSRR
                     GSQMTRQNFWHAVKRYAMKAGIHRSISPHTLRHAFATHLINHGADLRAVQMMLGHADL
                     STTQIYTHVAQARLKDLHRTHHPRG"
     gene            11295..>11657
                     /locus_tag="AC16PP_11715"
     CDS             11295..>11657
                     /locus_tag="AC16PP_11715"
                     /inference="COORDINATES: protein motif:HMM:NF021896.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide isomerase DsbC N-terminal
                     domain-containing protein"
                     /protein_id="HIR6482340.1"
                     /translation="MKLSFRALWVGAVLACLAVIGHADEAAEADEVKDVTDAKGGQAL
                     VERLTALRPGIPIEQVRETPLAGIYALELKGGSFFYGTADGKYLFAGDMYELTDTSLV
                     NLAEAGRTAKRQALMAQVT"
BASE COUNT         2207 a         3463 c         3912 g         2075 t
ORIGIN      
        1 tgcatcgagt ataacgcgac tgcgcggccc gtcgcaggcg gcttcgggca actcagcccg
       61 gaggaaactc gatgacgttg tcgctccgtc ggggcggttt tctggtcaga tcttcctcga
      121 gcgtttccgg cgccaccagc gatctgtgca ggtcggcgag cgagcgagcc atcatctccg
      181 agatgcggat ggtcgcggcg agaggtgttg ccgcacggcg ccgttccatg tccacacgga
      241 actgcagacc ctgtagtcgg cgtctggtgt cgtcgcagct ggcttcagca atgacggcgt
      301 cagtcagtga gcgtcgcaac gattccagtc ggtctggatc gttgcgagcc atatccacca
      361 gcgtatcgaa gcccggcagc cgggatttca taagcgaccc tactcgctag ttatcgttgt
      421 ggctcaaggt cgtgctggag gttccccgca gaaaactatg cctgtgggtg gacgttatcg
      481 ccacgtaatt tgtgtaaggg ctttgtgaac cggcggttgg tgcattactc tgccgctttg
      541 gggttgggtg acgcagcagt gctttatcag ctcggtgaac gccgtgtatg cgccgagggt
      601 gcgcatttca ttgcaccagg ggccatgctc acaggatcgg tgaccttgaa agccaacgtg
      661 aacgtctggt tcaacgcggt cattcgcggt gacgtggaac cgatcttggt cggtgccaac
      721 agcaacatcc aggactgcgc ggttttgcac accgatgcgg gctgcccgct gacgatcggg
      781 gactacgtca ccgtggggca caaggccgtt ctgcacggtt gcgacgttgg cgacaacacc
      841 ctcatcggca tcaacgccgt cgtgctgagc ggggcccgga tcggacgcaa ctgcatcatc
      901 ggggcgaatg cgctggtaac cgagggcaag gtcattcctg acaacagcat ggtgctgggg
      961 tcgcccggca aggtggtcag ggagctggtt gatgagcaga ttgcggggct gcgagtgagc
     1021 gctgaaggat atgtcgagcg cgcccggtgg tatcttgccg agttatccga acaggaaagt
     1081 gtggacatca cgtcatgacg agcataggga cgccgctggc gggcaacgca acgcgagtaa
     1141 tgatgctcgg tgccggcgag ctcggtaagg aagtggtcat cgagctgcag aggctcggcg
     1201 tggaggtcat tgccgtcgac cgatacgcca acgcgccggg aatgcaggtc gcccatcgca
     1261 gccacgtgat cagcatgctc gacggcgacg cgcttcgtga ggtgatcgcg ctggagaagc
     1321 ccgacatggt ggtccccgag atcgaagcca tcgccaccga ggtcctggtg gagttggaat
     1381 ccgagggcct gacggtcatc ccgacggcca gggcagcccg gctcacgatg gaccgcgagg
     1441 gcatccggcg cctggcttcc gagaccctgg gcgtgcgcac ctcgccctac cgatttgcgg
     1501 cgacgcgcga agagtacgac gaggccatac aggagatcgg cgttccctgt gtggtcaagc
     1561 ccgtgatgag ctcctcgggc aagggccagt ccacggttcg cacggcggcc gacgctgccg
     1621 ctgcctggga ctacgcgcag gccggggcca ggggtggtgc cgacaaggtg atcgtggagg
     1681 gtttcgtgga tttcgattac gagatcacgc tcctcaccgt ccgccatgtc ggaggcacgt
     1741 cgttctgtgc gcccatcggg catcgtcagg ccggcggcga ctatcaggag tcctggcagc
     1801 ctcagcccat ggccgaagcg gcgttggcgg aatgcaagcg cataggcgag ctggtcaccg
     1861 gtgagctcgg aggtcgtggt ctcttcggta tggagttttt catcgcaggc gaccaggtct
     1921 atttcagcga ggtgagcccg cgcccccacg acaccggaat ggtgacgctc ctaagccagg
     1981 atctctcgga gtttgcgctg cacgcccggg ccattctcgg attgccgatt cctcacatcc
     2041 agcagcacgg gccctcggca tccagcgtcg tgctggtgga gggagaatcg cgcgccgtca
     2101 ggttcgaggg gttcgaagcc gcgctcgcgg agccgggcac ggatttgcgt ctgttcggaa
     2161 agcccgaggt agcgggcgag cggcgcatgg gcgtcgcgct ggcccggggg cgggacgtgg
     2221 acgatgcccg caagaaggcc aacgcggcag ccgcagccat tcggtggtcg tttgacgact
     2281 gatcagcagg gttggcaaat cctccccggc tggctatagt cgcggccacg attcgggagc
     2341 tgaattgaac tctcatcacc gtccaaatct gtctcttctg gcgctgctgc gactgccggc
     2401 gtagccggca aatacttacg caaaagggca ttccgcccac cccatacgaa accttaacga
     2461 tcctccggga ccagcagcag cgagaaagac ctcgcaggcg aagaacaatg agtgagcaaa
     2521 cccaatcccc cttccgggcc cagcgccagc caaccggcgg tcgcgcggcg aacaccatgc
     2581 ctttccaaaa gtaccgtgcc tttcaggcca tcgacctgcc ggaccggcaa tggccgaccc
     2641 ggaccctgac cacggctccg cggtggtgca gcgtggatct gcgcgacggc aatcaggcgc
     2701 tgatcgaccc gatgaacgtg gccgagaaac tgcgaatgtt cgatctgctg gtcggtgtcg
     2761 ggtttgctga gatcgaagtg ggctttccgg ccgcctcgca gcccgatttc gatttcattc
     2821 gccgcatcat cgacgagggc aggatcccgg acggcgtcgc cattcaggtg ctctgccagg
     2881 ctcgcgagga gctgattgcc agaaccgtgg aggcgcttcg tggcgcgccg aaggtgatct
     2941 ttcacctata caactcgact tcggaattgc agcgccgcgt ggtgttcgag atggaccgct
     3001 ccgggatcat cgacctggcg gtgcggggaa cgcgcctggt caaggcgggc ctcgagaccc
     3061 tggattccga tatcacgttc gagtactcgc cggaaagctt taccggtacg gagctcgact
     3121 ttgccgtgga catctgcacc gccgtggccg aggagtgggg cgccaccccg gacgatccga
     3181 tgatcatcaa cctgccgtcg acggtggaga tggccacacc caacatctac gccgatcaga
     3241 tcgagtggtt cgcacggaat ttctcgaggc gggacagcac ggtgatcagc cttcataccc
     3301 acaacgatcg cggcaccggg atcgccgcta cggagcttgc gctgatggcg ggcgccgagc
     3361 gggttgaagg gacgctgttc ggtaacggcg agcgcacggg aaactgcgac atcgtcacca
     3421 tggcgatgaa tctcttcagc cagggcgtgg accccgagct cgacttccgt gacatgccgc
     3481 gtatccgggc gacggtggag gagatcaaca agctgccggt tcacgagcgc catccctatg
     3541 cgggagatct ggtgttcacc gccttctccg gctcccacca ggatgcgatc cgcaaaggca
     3601 tggccaaggt ggacagggat cactgggaag tgccctacct gcccatcgat cccgccgacg
     3661 tcggttcctc ctacaaggaa acggtccggg tgaacagcca gtccggcaag ggcggggtgg
     3721 gttttctgct cgaggagcac ttcggcatag ccttaccgcg ggaaatgctc gtcgagttca
     3781 gcgccgtggt gcagcagctg accgagagtc agggccggga gatcaagacc gacgagatcc
     3841 tcgaggccct gaagaaagag tatgtggtcg attccggtcc ctatcggctg gtcgacttcg
     3901 acctgctcac cggccggacg tctgatcagc gctgtattgc gcgggtagag gtcaacgagc
     3961 agcaggtgac catcgacggt gaagggtccg gccccatcga ggcgttcgtc aacggcatgg
     4021 tcgagacgct caacgagcct ttgaacatca ttgactatca cgagcacgcc atgacgacgg
     4081 ggaaggacgc cgatgcgttc tgtctggtcg ccatcgatga tcccaagcag gggcgctgct
     4141 acggcgtcgg cgtgagccgg aatacgatta ccgcgtcgtt catggcgatc gtctcggcgc
     4201 tgaaccggcg ctggaaggcc tgaagacggg ctggcttggc tgcgagggcc ctggcgggcc
     4261 tcgccccggt ggtggccgac gactcactcc acccgggtaa tcttcagcgt cttgatcgcg
     4321 ttttccttga tgtgcagggt ctcgatcagg tactcgccca gccgcagaca gaggttgctg
     4381 tcgggcaacg tttccagatg ttccagaacc aggccgttga gggtcttggg gccatgagtg
     4441 ggcagatccc agcccaggct ccggttgatg tcccgcagaa gcgccatccc gtcgatgaag
     4501 aacgagccgt cgtcctgagg atggatttcc tggatgctcg cagcaaagtc cgtcgtgaac
     4561 tcgccgacga tctcctcgag gatgtcctcg agagtgacga tgccctgcac ttcgccgtac
     4621 tcgtccacca ccaaggcaat ccgctgcttc tcgttctgga aattgatgag ctgggtgggc
     4681 agaggcgtcg cctcggggac gaagtacggc tcgtctgtca ggctcatcag atcgcttttg
     4741 ctcagctctt cctcgcgctc cttgcgctgc aggtagcggg cgaccttgcg cagatgcaga
     4801 atgcccagga tgttgttgat gttttccttg aagaccggca gccgcgtgtg ctggctgttg
     4861 ccgatctgga cgagaatgtc gccgatgtcg gcatcgacgt cgatcccgac gatctcgctg
     4921 cgcggcacca tgatgtcgtc gacggaaacc ttttccaggt ccaggatgct cagcagcatt
     4981 tcctgtccct gaccgggcag gccgacgcct tctcccacga cggtgcggag ctcctccacg
     5041 ctcaagctat ccgcggcgat ttcgccctcc ttcggcagga agggtttcac gatggcgttg
     5101 gaaatgccgt tgatgaaaac cacgccggga tacagcagcc tgagcagcgg ctcgagcagg
     5161 tacgctgagg gaaaggcgat ccgctcggga accttggcgg cgatggtctt gggtgctacc
     5221 tcggcgaaaa tgaggaagat cacggtgatg acgatggggg cgagcacgac gccggtatca
     5281 ccgaaaaagc gcaggccgat cacggtcgcg atgctcgcgg ccgtgaagtt caccaggttg
     5341 ttgccgatca gaatgacgcc gagcaaccgg tcgggacgcc gcagcagccg gtttgccttg
     5401 cgggcgccgc ggtgcttctg cttcacgaga tgcttcaggc gataccggtt cagagcgatc
     5461 atcgcggtct cggaactgga aaaataggcc gacagggcgg tcagcacgaa tacgaagatc
     5521 agcaggacgg aagcgggcaa ctcggtgagg gacatcaggc gtccagcagt atctcgatga
     5581 cgaacttgct accgaagtag cctagcacga gcagcaggaa ggcgatcaac gtccagcgaa
     5641 cggccgtcgt gccgcgccat cccagcagct tgtgccctgc cagcaaggtc gcgtagagca
     5701 gccaggacag ggacgagagc accgtgtgat ggacgacgcg ctgcgcgaac atgtcctcga
     5761 gaaaggcgaa accgctcagt atagccaccg tgagaacgac gaggccggtc cagagcatcg
     5821 aaaacagaag cgtctccatg gtctccagcg gcggaagaag acgtatgaac gccaggccgt
     5881 ggcgcgctcg caggtagtgt tcctgaatcg ccagcagaac ggactgacag gcggccatga
     5941 acaggatgct gtaggccgtg atggagatga gaacgtgcag cagcagttcc cagggcatct
     6001 cctgccgcgg aacgaaagcg gattctccga gcagcgagcc gatgatgccg agagcgctgg
     6061 ccggaaatat caggacgcac aggttgtgaa cgggcagcct gagggaggcc agcatcacga
     6121 atgccgtcat caccagcgtg accaggccgc cggtgctgaa aaaaccgagg tgaattcccg
     6181 ctggagtgta gatcacgctg atcgtggtta gggcgtgcag aaccagagcg gggatcccca
     6241 ctgcgatgag cggctgcaac agcacgggag ccgatcgcga cagtcgtcgc agctgcatcg
     6301 cggtgcccag gaggtacagc aacacggcgc acgttccggc ggcgatgctc gtagttgtgg
     6361 caaccatttc ggtcggcgtc gtcgatggct ggagcgtcgg ggagtttggc ataacgcacc
     6421 aatagcgtga agggctataa tcggcccgcc gccgatggtc ccggcgtgcg tccaacggct
     6481 cgacgaggtt ttcatgttcg acaatctttc cgaacgactt acccagacgc tccgcggcat
     6541 ctcgggcaag gcgaagctca cggaggaaaa cgtccaggac gccttgcgcg acgtgaggat
     6601 ggccctcatc gaggccgacg tcgcgttgcc ggtggtgaaa gactttctct cccaggtcca
     6661 gaccagggcc ctgggccaga cggtttcgtc tgctctcaat cccggcgagg agttcgttcg
     6721 gatcgttcac gaagagctag tccacgtgat gggcgatgac aacgacgccc tcaacctggc
     6781 caccacgccc ccggccgtcg tgctcatggc cggcctgcag ggcgctggca aaacgaccac
     6841 ggttgccaaa ctcgcgctgc tcctgaaaga aagggagcgc aagaaggtgg cggtggtcag
     6901 tgccgacgtt taccgtccgg ctgccatcga gcagctcgcg accctggccg gggaggtcgg
     6961 cgtgatctgt gttccatcca gcgcgcagga gaagccgctg gagatcgccc gtcgtgccgt
     7021 tgatgaagcc cgcaaccagt ttgccgacgt tctgctcgtg gacaccgcgg gacgccttgc
     7081 gatcgatgcc gacatgatgg cggagatcaa gctgctgcac gaagagctcc agcccatcga
     7141 gacgcttttc gtcgttgacg ccatgaccgg tcaggacgcg gccaacacgg ccaaggcttt
     7201 caacgatgcg ttggcgctaa ccggcgtcgt gctggcaaag gccgacgggg atgcccgggg
     7261 cggtgccgcc ctgtcggtcc gcgccatcac gggcaagccc atcaagttca tcggcatggg
     7321 cgaacgggtc gatgccctgg agcctttcta tccggaccgc atggcatcgc gaatcctcgg
     7381 catgggcgat gtcctcagcc tcatcgagga agccgagcgc aaagtcgaca agcgcaaggc
     7441 acagaaactc gccaagaaga ttcagcgtgg tcgcaagttc gacctggagg acttccgcga
     7501 gcagctcgat cagatgtcca atatgggagg cgtttcgagc atgctggaga agctgccggg
     7561 catggggcag ctgccgcccg cggcgcaggc tcagctggac gacgggatgt ttcgcaagat
     7621 ggcggtgatc atcgactcca tgacccccca cgaacgccgc ttcccggacg tgatcaccgg
     7681 atcccgcaag cagcgggtcg cgcggggcag cggcactcag gtgccggatg tgaaccgcct
     7741 gctcaaacag cacaagcaga tgcagcgggt gatgaagaag atgtcgcgca aaggcggcat
     7801 gcagcgaatg atgcggggca tgcaggggat gcggccgccg cccggcgtgc gtcggtgatg
     7861 gagcctcacc ggccttgcat gagccgggcg ttccattaaa ataccgcgcc tttcaacgat
     7921 tgggcccgcc tggcgggccc gcagcagggt catcacatgc taacgattcg cctggcacgc
     7981 cacggatcca agaagaaccc cttctatcac atcacggtcg cggatcgcac ggcgaagcga
     8041 gacggcagat tcgtcgagcg ggtcggcttc tacaatccgg tggccaaggg tcaggccgag
     8101 gaactgcgcg ttgatctcga gcggatcgac tactggctgt ccgttggcgc gctcgcgtct
     8161 gatccggtca cccggctgat tcgtcgggca cggcggcagg aagccgccgt ggaagccacc
     8221 gccgactgac gggccgcgtc gggcgcccca cggcgggcgt tgccggggat cccggagtct
     8281 tccgcatgca ttcccaggaa tacgtcatcg tcggccgctt cggcggtcct tacggtgtgc
     8341 acggctggat ccgggtcacc tcgttcacgg atccgcccgc caaccttctc ctctacgggc
     8401 cctggcacgt ggagcgtgac gggcaatggc acgcctacca cccggtccaa gtgcggcccc
     8461 accagcaggg cttgatcgcc tgctacgcag acatcccgga ccgcagcgtg gcggccgcgc
     8521 tcaccgggcg gttcatcgcc gtaccccggg actgtctgcc ggtgctggac gcggaagagt
     8581 attactggcg agacctggaa ggtctcgagg tatggaaccg cggggcgcgg ctcggcgtgg
     8641 tggatcatct catcgacact ggcgcgaact cggtgctggc ggttcgaggc gactcgacgc
     8701 agacgctgat tcctttcgtc agccggtttg taaccggggt cgatcttgcc gcgggcagga
     8761 tcgacgtcga ctgggaagag gccgactaga cgtgtggtgc ggcatcgtca gtctttttcc
     8821 ggagatgttc cgacggctct ccgagctggg cgtgctcggc agagcgatcg atcgaggcat
     8881 cgtgaccctc gagctcttca acccgcgcga attcgcccgg gaccgacacc gcacggtgga
     8941 tgaccggcca tacggcggcg ggcccgggat ggtcatgcag gtagagccgg tcgttgcggc
     9001 cgtcagcgcg gcgcgggagg cggcccgagc cgccctgggt gagagcgtgc cggtgatcta
     9061 cctgagcccc cagggtcggc ggctgacgca gcagagagtc agtgaattgt ctcagcagcg
     9121 cggggtgata ctgctggcgg gtcgttacga gggcatcgac gagagggccg tctcgctggc
     9181 ggtggacgag gaagtttcca tcggcgacta cgttctcagc ggcggcgagt tgcccgcgct
     9241 ggtcgtgctg gacgccgtca cgcggctgct gccggggact ctcggcaatt cggcctcaat
     9301 agaggctgaa tcacaccttg atggattgct cgattaccct cactatacgc gccccgagaa
     9361 cacccacggc ctgggtgtgc ccgaggtcct gctcagcgga gaccacgagg cggtaagccg
     9421 ctggcggcgg aagatggcct tgctgcggac ctggcagcgg cgccccgatc tgctggtcgg
     9481 gcggcagctg gatgatgagg acagcgcgct cttgcgcgag tgttttgaag aattggccgc
     9541 tcggaccgag tcgggcgagg agtagaagat cgtgcgcaga aacaaagtca tcgaggagct
     9601 cgagtcggag cagctgaagt ccgacctgcc ggagttcggg ccgggagaca cggtcgtcgt
     9661 gcaggtcaag gttcgtgagg gtaaccgaga acggctgcag gccttcgaag gcgtggtgat
     9721 cggcaagcgc aatcgcgggc tgaactcggc attcacggtt cgcaagatgt cccatggggt
     9781 cggtgtcgag cgcacgtttc agagtcacag cccgatgatc gcttcggtca gtgtcaaacg
     9841 tcgcggcgac gtccgtcagg ccaagctcta ctacctgcgt gagcgctcgg gccggtctgc
     9901 gcgcatcaag gaaaaggtct aacccgtcgt ttcaccgccc ggcggcttaa aaaaaatgaa
     9961 gccagcttgc cgggttgggg gagttggggg gaggccccgg caagctggcc gtcttcggaa
    10021 accggtggag agggggccgg cttccttcag cgtgggtcac gatcactcca ggggacagct
    10081 gggtgggtgg atcgcgcccg tataagccaa tgcagctacc gtgccagcta tctggattcc
    10141 gttcaaaata gattaaaaat ccttttgttt catgtagttg aataactatc cgctggtttt
    10201 tatgtcggtt gactgaggac caactacgtc acaaagtgtc cgtgatgggt gcccaaggag
    10261 acgaaatggg cgaggttctg ccgaaggatg aggcggcccg ccatacggtg gacgaatatc
    10321 tcgacgcggt ctggctcgag cggggcttga gcgagaacac gctggccgcc tatcgtcggg
    10381 atctctacca gcttgccgac atgctgaagc gggatccgct gacagccaac gacgcggatc
    10441 tgctcgagtg cctggcgctg cggcatcgtc agggttaccg cgccagctcc gccgcccgct
    10501 ggctctcctc cgtgaaaggc ttctaccggc acgcggccgg gcgcgggcgg atcggcgcgg
    10561 atcccagcgc tcacctggag catccgaaga tgggacgccg gctgccgggc tcgttgtcgg
    10621 aggcggaagt tcgtcagctg ctggcagcac cgcgcctgga cgatcccatc gggctcaggg
    10681 acaaggccat gctggaactt ctctacgcct cggggttgag gatatccgag ctggtgggtc
    10741 tcacgatgag cgccgtgaac gcgcggcagg ggatcgtgag aatcgtcggg aaggggggca
    10801 aggagcgcct ggtgcccatg ggtggggagg caatgcgctg gctcgagcgt tacctccacg
    10861 agggccgtcc gctcatcaag ggcagcgatg ccacggcagc gatgtttccg agccgccggg
    10921 gctcgcaaat gacccgacag aacttctggc acgcggtgaa gcgctatgcc atgaaggcgg
    10981 gcatccaccg gagcatctcg ccgcatacgc tgcgccatgc ctttgccacc caccttatca
    11041 atcacggggc ggacctcagg gccgtgcaga tgatgctggg acatgcggac ctgtccacga
    11101 cccagatcta cacgcacgtc gcgcaggctc ggctgaagga cctgcatcgg acccatcacc
    11161 cgcgaggatg agagcgaagc gcgggtttga gtattggagc aggctatttg taaaggaatg
    11221 tggttaagct ggatatttgg gcgcgctcgc tggtctaatt ctccggccgt gcaggaggca
    11281 ccgtgaagaa atcaatgaag ttgtcatttc gggccctctg ggtcggggca gtgctagcgt
    11341 gcctggccgt catcggacac gccgacgagg cagccgaggc cgatgaggtc aaggacgtca
    11401 cggacgccaa aggaggccag gcgctcgtcg agcggctgac ggcgctgcgt cccggtatac
    11461 ccatagagca ggtacgcgaa accccgctcg ccggcattta cgccctcgag ctcaagggcg
    11521 gttcgttttt ctacggtacc gcggatggta agtatctgtt cgccggagac atgtacgagc
    11581 tcaccgacac cagcctggtg aacctcgccg aagcggggcg aacggcaaag cggcaggccc
    11641 tgatggccca ggtgacg
//
LOCUS       DBNHKV010000365         3535 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_618116, whole genome shotgun sequence.
ACCESSION   DBNHKV010000365 DBNHKV010000000
VERSION     DBNHKV010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_618116"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="AC16PP_11720"
     CDS             complement(<1..1401)
                     /locus_tag="AC16PP_11720"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6482341.1"
                     /translation="MRSIRPLIIALAAALPLAAHGDPFEHEWCVYPTDHFEILSDLRP
                     RQIKRLAAELEVFRQTAAQFIPGTGAANLPIRIVVFRDRDDFRETMNSPKFGGFMQPS
                     LRGATLVLGPQDANRLLHTTARHEYSHYLLRNRIEVSFPVWYDEGLASFLSTMSTRRR
                     SVVVGEAPARHLDSRLRRSAVGLAELIEERDVYTWEPNKLNDFYTGAWTLVHYLTLGH
                     LAGFEDRRERLSRYLNEVVTPFPAAMDLSYDQLEKELLTYTNRRKLPTIELKRPESSA
                     ARADVGCLRDDERDYELAVAMIKKRPETALDIFAGLQRKHPADVRHLVGLATASAELN
                     RPEDAKGYAAAALKLAPDDPAAQIEYGTRLIRNCIMMRALGCDERWQQAVKLFRSGVR
                     ADPERFDAVMGLGLSYLHSGRPGDALNYLKIAYQKAPWAPHLNFYLGETYRLVGDPRA
                     RQHLLNARNWSDQDVWR"
     gene            1458..2015
                     /locus_tag="AC16PP_11725"
     CDS             1458..2015
                     /locus_tag="AC16PP_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482342.1"
                     /translation="MAYGWIVLAGISLAVVDGGLARAEAPPEPDHLPAEAASGLPPLR
                     CEAEQTGGFHDYPGEDEGYEPALFNPSRIFLEENVVFMLNLPVGNGAPDLYLVMRVGE
                     ELETELACRQVRGADGAMGYSCVNTPPSEMLLINRETLRFTRTAVGGWTFAGASESLS
                     GDSIFIEYGACVPDDDQVRRPKFSP"
     gene            complement(2005..2241)
                     /locus_tag="AC16PP_11730"
     CDS             complement(2005..2241)
                     /locus_tag="AC16PP_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482343.1"
                     /translation="MRTLILAACCLIAGIATADEAVVKVYSLSVQERLESLELIDVAA
                     EKAPAGSDAPMDAELAEILREVEALEQASALVRD"
     gene            complement(2273..2785)
                     /locus_tag="AC16PP_11735"
     CDS             complement(2273..2785)
                     /locus_tag="AC16PP_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482344.1"
                     /translation="MSDHESMSMGIARLAAAGCLTVLTWVASPDAAAPSYDFADAAYL
                     APASSLPDRADTVERNDLEQRTIAECLADESVCQSRLIGIRHLLTKAQSLPIEKQIRL
                     VNRYVNKRRYRDDRSVGDYHAVLAVRSAPGSTWLLESDNTIQRGGAWGYRYVFAINED
                     SIWDHEGADG"
     gene            2884..>3535
                     /locus_tag="AC16PP_11740"
     CDS             2884..>3535
                     /locus_tag="AC16PP_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIR6482345.1"
                     /translation="MGAALLGVCVLMGCASADRSPMDSLDYDPRLLTAETVFADPVIP
                     SELPAVDILAIDGEMRAFVADAVGDVRNSSVRFKRLMRALVEAGYFNGSYYQPGLTLT
                     STELFAARTGNCLSYTTMFIALAREAGLDAGFQIVEVPPSWDADSGFLIRYTHINALL
                     SGVRGDVTDSGEVSVDFNAVHPDPHYPRRPVSDDDATALFYSNRSVDRMLNGDAVNG"
BASE COUNT          557 a         1110 c         1203 g          665 t
ORIGIN      
        1 gcgccacacg tcctggtctg accagttgcg ggcgttgagc aggtgctgcc gcgcccgggg
       61 atcgccgacc agccggtagg tctcgcccag gtagaagttc aggtgcggcg cccatggcgc
      121 cttctgatag gcgatcttca ggtagttgag cgcgtctccc ggccgcccgg agtggaggta
      181 ggagaggccg agtcccatga ccgcatcgaa tcgctcggga tcggcacgca cgccgcttcg
      241 gaacagcttc accgcttgct gccacctctc gtcgcagccc agcgccctca tcatgatgca
      301 gttccggatc aggcgggtgc cgtactcgat ctgagcggcg gggtcgtcgg gcgcgagctt
      361 caacgcggcg gccgcgtatc ctttggcgtc ctccggccga ttcagctcgg ccgatgcggt
      421 ggccagcccg accagatggc ggacgtctgc cgggtgcttg cgctgcaggc cggcaaagat
      481 gtcgagggcg gtctccggac gcttcttgat catcgccacg gccagctcgt agtcccgctc
      541 gtcatccctc agacagccga cgtcggccct ggctgcggag ctctccgggc gcttgagctc
      601 gatggtcggc agcttgcgtc ggttggtata ggtcagcagc tccttttcga gctggtcgta
      661 ggagaggtcc atcgccgccg ggaagggggt gacgacctcg ttgagatacc ggctcagcct
      721 ttcccgtcga tcctcgaagc cggccaggtg acccagcgtg agatagtgca cgagcgtcca
      781 cgcgccggtg tagaaatcat tgagcttgtt cggctcccac gtgtagacgt cgcgttcctc
      841 aatgagctca gcaagcccga ccgcgctgcg gcgcagccgg gaatcgagat gccgcgcggg
      901 cgcctcgccg accacgaccg accggcgcct ggtggacatc gtcgacagaa acgacgccag
      961 cccctcgtcg taccagacgg gaaagctgac ctcgatccgg ttgcgcagca ggtagtggga
     1021 gtattcgtgc cgggcggtgg tgtggagcag gcggttcgcg tcctgggggc cgagaacaag
     1081 ggtggccccg cggagagacg gctgcatgaa gccgccgaat ttgggcgagt tcatggtctc
     1141 gcggaaatcg tcccggtcgc gaaacaccac gatgcggata ggcaggtttg cggctccggt
     1201 gccgggaatg aactgggccg ccgtctggcg gaagacttct agctcggcgg caaggcgttt
     1261 gatctggcgc ggccgcaggt cgctgaggat ctcgaagtgg tccgtcgggt agacgcacca
     1321 ctcgtgctcg aagggatcgc cgtgggcggc cagcggcagg gcggcggcca gggctatgat
     1381 gagtgggcgg atactgcgca tgctcgacgg tcaccggaga tgggttttag actattcgag
     1441 tggcgcggag ttctgaagtg gcatacggat ggatcgttct tgcgggtatc tcgcttgccg
     1501 tcgtcgatgg ggggctggcc cgtgcggaag cgccgccgga gcccgaccac ttacccgccg
     1561 aggcggcctc cggactgccg ccccttcgtt gtgaggccga gcagacgggc gggtttcacg
     1621 actatcccgg tgaagacgaa gggtacgaac cggccctgtt caacccgagc cgcatctttc
     1681 tcgaggagaa cgtggtgttc atgctgaacc tgccggtggg caacggcgcg cccgacctgt
     1741 atctggtcat gcgggttggt gaggagctgg aaaccgagct cgcctgtcgc caggttcgcg
     1801 gcgcggacgg ggcaatgggc tacagctgcg tgaacacgcc gccatcggag atgctgctca
     1861 tcaaccgcga gacgctgcgc ttcacgcgca cggcggtagg cggctggacg tttgctggcg
     1921 ccagcgagtc cctttcggga gactcgatct tcatcgagta tggcgcctgc gtgcctgacg
     1981 acgatcaggt gcggcggccc aaattcagtc cctgaccagc gcggaagcct gttccagggc
     2041 ttccacctct ctgagaattt cggccagctc cgcatccatc ggcgcgtcgc tccccgccgg
     2101 cgctttctcg gcggccacgt cgatcagctc caggctttca aggcgttcct gaacggaaag
     2161 cgagtagacc ttgaccacgg cctcgtcggc cgttgcgatg cctgcgatca ggcagcacgc
     2221 tgccagtatc aacgttctca tgctctgctc ccatgccccg acctctccgg ggtcagccgt
     2281 ctgctccctc gtggtcccag atggaatctt cgttgatggc gaagacgtag cggtaccccc
     2341 aggcaccgcc cctctggatc gtgttgtccg actcgagcag ccaggtcgag ccgggcgcgc
     2401 tgcgcactgc cagcaccgcg tggtagtccc cgacgctgcg gtcatcccgg tagcggcgct
     2461 tgttgacgta ccggttgacc agacggatct gtttttctat cggcagggac tgggctttcg
     2521 tcagcaggtg gcgaatccct atgagtcggg attggcatac agactcgtca gccaggcact
     2581 cggcgatcgt gcgttgctcg agatcgttgc gctcgacggt gtcggcccgg tcgggcagcg
     2641 agcttgcggg agcgaggtag gcggcgtcgg cgaaatcgta ggagggtgcg gcggcatcgg
     2701 gggaggccac ccatgtcaga accgtcaggc agccggccgc ggccagccgc gcgatgccca
     2761 tgctcatgga ttcatggtcg ctcatggaag cacggtgtgc aatggtccca cggcggcgct
     2821 actatttttg cgggggtgcg ttccatgccg gtcgcgcaga gttcattttc agaggcctgg
     2881 aggatgggcg ccgcattgct cggcgtctgc gtgctcatgg gttgcgcgag tgccgaccgc
     2941 tccccgatgg atagcctcga ttacgacccg cgtctgctca cggcagagac ggttttcgcc
     3001 gacccggtga tcccatccga gctgcccgcc gtcgacatac tcgccatcga cggcgagatg
     3061 cgcgccttcg tcgccgatgc ggtgggcgac gttcgaaact cttccgtgcg tttcaagcgg
     3121 ctgatgcgcg ccctggtcga ggcgggctat ttcaacggca gctattacca gcccggcctg
     3181 acgctgacct cgacggagct ctttgcagcc aggacgggca actgcctctc ctacaccacg
     3241 atgttcatcg ccctggcccg ggaggccggc ctggatgccg ggtttcagat cgtggaggtc
     3301 ccacctagct gggatgcgga ttccgggttt ctgatccgct acacgcacat aaacgcgctg
     3361 ctgagcggcg tgcgcggtga cgtgaccgac agcggcgagg tgtccgtcga cttcaatgcc
     3421 gtgcatcccg atccgcacta cccgcggcgc ccggtctcgg atgacgacgc gaccgcgctc
     3481 ttctactcca accgatccgt ggaccggatg ttgaatggcg atgcggtgaa cggat
//
LOCUS       DBNHKV010000366         3373 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1114743, whole genome shotgun sequence.
ACCESSION   DBNHKV010000366 DBNHKV010000000
VERSION     DBNHKV010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1114743"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="AC16PP_11745"
     CDS             complement(<1..892)
                     /locus_tag="AC16PP_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450 family protein"
                     /protein_id="HIR6482346.1"
                     /translation="MTEPAAVRAEIPDPYSLPLDTLNVAQPALFQNDAHWGYFERLRN
                     EDPVHYCPQSDFGPFWSVTKFKDIFYVDTHHEIFSSEGGITLGPPVNQPPEEGFRPSM
                     FIAMDPPKHDVQRATVTPVVAPRNLAKLESVIRERAGRILDSLPRGETFDWVDTVSVE
                     LTTQMLATLFDFPFEERRKLTRWSDVATAIPGAGIVDSNAQRREELLECLDYFTRLWN
                     ERVNSEPGNDLISMLAHGEETRHMQPMEFLGNLILLIVGGNDTTRNSISGGVLALNQN
                     PDQYRKLRENPGLIRTMVPEI"
     gene            1044..1859
                     /locus_tag="AC16PP_11750"
     CDS             1044..1859
                     /locus_tag="AC16PP_11750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03084 family metal-binding protein"
                     /protein_id="HIR6482347.1"
                     /translation="MSTAEQIELARQVDDLRAEGAELAELMSKLTEEDWQRRTTFKDW
                     TIYDVIAHLHFSDHMGMTTIESGAAFQALMADMQSSGLPFVEYTRRWLGSVSGADLLE
                     RWKTLLDELCDRLQALDPQERLTWAGPGMKPRMFTTARQMETWAHGWEIYDCLRLERM
                     HTDRIKNIAAIGVRTFGWTFVNRGLELPGDPPFVRLTAPSGAIWEWSDPNADSSVTGS
                     AVEFCQVVTQVRNVADTKLEVTGKPAREWMAIAQCFAGAPEDPPAPGSRRPAG"
     gene            1885..3066
                     /locus_tag="AC16PP_11755"
     CDS             1885..3066
                     /locus_tag="AC16PP_11755"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6482348.1"
                     /translation="MSGPLSGIRIIELATMIAVPGATHLLATQGAEVIKVEDPVRGDE
                     LRHYGSEKGGMSAWFINANSGKRSIALDLQHEDGKSVLWRLLETADVVMQGFRPGVIG
                     RLGFDSETVRDRLPAIVYCSSSGFGPHGPYAQLPVYDPVIQALSGWAGAQRYDEGPRL
                     VRGMVADKVAALTSAQAVTAALLQRSMTGRGLHVELSMLEANIAFNWSDVMMHASLLD
                     ADATHRPNLLSSYRLFPCRDGWVTVTAGTDAQWRAFSQALERPELAEDARFVTASARA
                     GHVADWYEAMGEAVAAFRLQEVLDRLRTADVPVAPVLDPTEVAEDPQVLAAGLLREVD
                     HPVAGRLRQPRPSAEIFGSSQDLIPAPTHGQQTAQVLAEIGYDEGAIGRLAADGVVKV
                     D"
     gene            complement(3063..>3373)
                     /locus_tag="AC16PP_11760"
     CDS             complement(3063..>3373)
                     /locus_tag="AC16PP_11760"
                     /inference="COORDINATES: protein motif:HMM:NF024591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR6482349.1"
                     /translation="GGENIYSTQVEAAIHKHPAVLEAAVIGVPDDEWGEAVKAVVVLK
                     PGETATEQEIIDTARENLASYQKPKSVDFVDSLPKAPTGKILKRELRDPYWKDQDRNV
                     "
BASE COUNT          598 a         1063 c         1108 g          604 t
ORIGIN      
        1 tgatctccgg gaccatggtg cggatcaggc cgggattttc ccggagcttg cgatactggt
       61 cggggttctg gttgagtgcc agaacaccgc cactgatcga gttccgcgtg gtgtcgttac
      121 cgccgacgat gagaagtatc aggttgccca ggaactccat gggctgcatg tggcgggttt
      181 cttcaccgtg agcgagcatg gagatcagat catttcctgg ctcggagttg acccgctcgt
      241 tccacaaacg cgtgaagtaa tccagacact ccagcagctc ctcgcgccgc tgcgcgttgc
      301 tgtcgacgat gcctgccccc ggtatcgcgg tcgcaacgtc cgaccaacgc gtgagcttgc
      361 ggcgttcctc gaacgggaag tcgaagagcg tcgcgagcat ctgcgtggtc agctcgacgg
      421 aaaccgtgtc tacccagtcg aacgtctcgc cgcgcggcag agaatccaga atccgcccgg
      481 cgcgctcacg aatgacgctc tcgagcttgg ccaggttgcg cggggcaacc acgggggtca
      541 cggtcgcccg ctgcacatcg tgcttgggag gatccatggc gatgaacatg ctcggacgga
      601 atccttcctc cggaggctgg ttgacgggcg ggcccaacgt gatcccgccc tcggatgaaa
      661 agatctcgtg atgggtgtcc acatagaaga tgtctttgaa cttcgtcacc gaccagaagg
      721 gaccgaaatc gctctggggg cagtagtgca cagggtcctc gttgcgcaac cgctcgaagt
      781 aaccccagtg ggcgtcgttt tgaaacagcg ccggctgggc cacgttgagc gtgtccagag
      841 gcagggaata cggatctgga atctcggctc ggacggcagc gggttcggtc atgcgtcggc
      901 cctcgaatcg gaatctcgcc ggatgcttca tggtagggcc agcgctttgc gacgtctacg
      961 cgacgtcctg agccgggccg ggcggatgag ccgcgccaac acgcctggta tatgatggcc
     1021 cctcagcttc gggatccaag atcatgagca ccgcggaaca aatcgagctt gccaggcagg
     1081 tagacgacct gcgcgccgaa ggcgccgagc tggccgagct gatgtccaag ctgacggagg
     1141 aagactggca gcgcagaacg accttcaaag actggacgat ctacgacgtc atcgctcatc
     1201 tgcacttctc tgaccacatg gggatgacca ccatcgaaag cggcgccgcg ttccaggcgc
     1261 tgatggcaga catgcagtcc tcggggctcc cgttcgtcga gtacaccaga cgatggctgg
     1321 gcagcgtatc cggcgcggat cttctcgagc gctggaagac tctgctggac gagttgtgtg
     1381 atcgtctcca ggccctggat cctcaggaac gcctgacgtg ggcgggcccc ggcatgaaac
     1441 cccggatgtt caccaccgcg cgccagatgg agacgtgggc ccacgggtgg gaaatctacg
     1501 attgtctccg gctggaaagg atgcacaccg accggattaa gaacattgca gccatcggcg
     1561 tgcgcacttt cggctggact tttgtcaatc ggggcctgga attgcccggg gatccgcctt
     1621 tcgttcgcct gaccgcccct tccggagcta tctgggagtg gagtgatccg aacgcggaca
     1681 gcagcgtcac cggcagcgcg gtggagttct gccaggtcgt cacccaggta cgcaacgtcg
     1741 ccgatacgaa actcgaggta accggcaagc cggccaggga gtggatggcc attgcccaat
     1801 gctttgccgg tgctcccgag gatccgcctg cgcccggcag ccgccgcccc gcaggttgaa
     1861 acggcgtcag catcggaggt ttgcatgtca ggacccttga gcggcatacg gatcatcgag
     1921 ctggcgacga tgatcgcggt ccccggggcc acgcacctgc ttgcaaccca gggggccgag
     1981 gtcatcaagg tggaagaccc ggtgcgcggt gacgagctgc gccactacgg cagcgagaag
     2041 ggcggcatga gtgcctggtt catcaacgcg aactccggca agcgctcgat cgccctggac
     2101 cttcagcatg aggacggcaa gagcgtgctc tggagactcc tggagaccgc cgacgtggtg
     2161 atgcagggtt ttcggcctgg cgtgatcgga agactcggct tcgacagcga aacggtcaga
     2221 gaccgcctac ctgccatcgt ctactgctcg tcatcgggct tcggccccca cggcccctac
     2281 gcccagctcc cggtctatga ccccgtcatt caggcgctgt ccggctgggc cggagcacag
     2341 cgctacgacg aggggccgcg cctggtgcgc ggcatggtgg ccgacaaggt ggccgctctg
     2401 acgtccgccc aggccgtgac cgcggctctg ctgcagaggt cgatgacggg tcgcgggcta
     2461 cacgtcgagc tcagcatgct ggaggccaac atcgccttca actggtccga cgtcatgatg
     2521 cacgcttccc tcctcgatgc ggatgcgacc cacaggccca acctgctttc ctcctatcgg
     2581 ctgttcccct gtcgcgacgg atgggtcacg gtgaccgctg gcactgacgc ccagtggcgg
     2641 gccttcagcc aggcgctgga aagaccggag ctggcggagg acgcccggtt cgtcaccgcg
     2701 tcggcgcgtg ccgggcacgt ggcggactgg tacgaggcca tgggcgaggc cgtggccgcc
     2761 ttcaggctcc aggaagtgct cgaccgcctg agaacggccg acgtgcccgt ggcacccgtg
     2821 ctcgatccga ccgaagtcgc cgaggatcca caggtgctgg cggccgggct gctgcgcgag
     2881 gtcgatcatc cggttgcagg aaggctgcgc cagccccgac cgagcgccga aattttcggg
     2941 agctctcagg acctgatccc cgcgccgacc cacgggcagc aaacggcaca ggtgttggcc
     3001 gagatcggct acgacgaggg cgccatcggc aggctggcgg ccgatggcgt cgtcaaggtc
     3061 gactagacgt tccggtcctg gtccttccag tagggatccc gcagctcacg cttgagaatc
     3121 ttgcccgtgg gagccttcgg caggctgtcg acgaagtcga cggatttcgg tttctggtaa
     3181 gaggccaggt tctcccgggc cgtgtcgatg atctcctgct cggtagcggt ctctccgggt
     3241 ttgagcacca ccaccgcctt cacggcttcc ccccactcgt cgtcgggaac accgatcacc
     3301 gccgcctcca gcacggccgg atgtttgtgg atggccgctt cgacctgggt cgagtaaatg
     3361 ttctcccctc cgg
//
LOCUS       DBNHKV010000367         3117 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_50114, whole genome shotgun sequence.
ACCESSION   DBNHKV010000367 DBNHKV010000000
VERSION     DBNHKV010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_50114"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="AC16PP_11765"
     CDS             <1..609
                     /locus_tag="AC16PP_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482350.1"
                     /translation="YLAVPSYTFVRFGMWQEALAVPEPEEDLVIAAFMRHYGRGVAHA
                     ALDDTGSAKAELAAMEAILASGEFRAQMDRQGDIGDILASIASSLVRARIARADGDVD
                     AEIRHLRDAVASQDSLPYSEPPYWHYPVRQSLGSALLRLGDADGAAAAFTEDLGKFPK
                     NGWSLHGLAEARRALGKPTDELEAAHREAWQYADIEPTAGAE"
     gene            634..1893
                     /locus_tag="AC16PP_11770"
     CDS             634..1893
                     /locus_tag="AC16PP_11770"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6482351.1"
                     /translation="MSEALAPIDLTTADPYSVPLDQIDMSQAALFQTNTFGPYFERLR
                     REDPVHYTADSFFGPYWSITRFDDIVYVDKHHELFSSEDGITLADRQQDFATVNFIAM
                     DPPKHDDQRKAVQGVVAPRNLAKMEALIRGRVADILDSLPLGETFNWVDRVSIELTTQ
                     MLATLFDFPFEERRKLTYWSDMATSSELAGGPTPEPVRREALMECLEAFTRIWHERAS
                     QPPDESVDLISMLAHSDDTKDMLNRPMEYLGNLILLIVGGNDTTRNSITGGVLALNQN
                     PGEYDKLRQHPELIPNMVAEIIRWQTPLAYMRRTATQDTELGGKLIRKGDKIAMWYVS
                     GNRDETAIDQPEEFIIDRPNARHHLSFGFGIHRCMGNRLAEMQLRIVWEEILKRFHTV
                     EVVGEPVRVQSSFVKGYSQLPVRLHPV"
     gene            complement(1956..2639)
                     /locus_tag="AC16PP_11775"
     CDS             complement(1956..2639)
                     /locus_tag="AC16PP_11775"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR6482352.1"
                     /translation="MSTLAVAFTAGGAHAQPDSGASHQHAAPVVLAPGYADLDFAPPP
                     VGGYALPPLGEVADARVVDAQGVIGRLHEMLGDKVVVLSFIYTRCNDVNGCPLATHVL
                     GKLQGAITSAPDLQGKVRLVSFSFDPSHDTPAVLRQYGGHFRKPGFDWRFVTVANAAD
                     LTSVLDAYDQWVVRDIDADGEFVGTLSHILRVYLIDRDRQIRNIYSISFLHADTVAND
                     IRTVLLDGG"
     gene            complement(2664..>3117)
                     /locus_tag="AC16PP_11780"
     CDS             complement(2664..>3117)
                     /locus_tag="AC16PP_11780"
                     /inference="COORDINATES: protein motif:HMM:NF017505.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HIR6482353.1"
                     /translation="EGGEWQAKAVADIGDAAKIPLPVDISISAEDNLLWVNTWNDGLV
                     HLYDISDPHAPKQLFEQKIGEQVNMVSQSWDGERLYFTSSLLANWDKTGPSGPDLQYF
                     KLYGWDGEKMTPGFTIDFVAAGLGAPHQMRFGSYALYGKRPPQQVGLH"
BASE COUNT          572 a         1049 c          983 g          513 t
ORIGIN      
        1 tacctcgcgg tgccgtccta caccttcgtg cgcttcggca tgtggcagga agctctggcg
       61 gttccggaac ccgaggaaga tctggtgatc gccgcgttca tgcgtcacta cggccgcggg
      121 gtcgctcacg ccgccctgga cgatacgggg tccgcaaagg cggagctcgc ggccatggag
      181 gcaatcctgg catccggcga gttccgggcc cagatggatc gccagggaga catcggggac
      241 atcctcgcca gcatcgcctc gtccctcgtg cgcgcccgga tcgcaagagc agacggggac
      301 gtggacgcgg agattcggca tctgcgcgac gccgtcgcaa gccaggactc gctcccgtac
      361 agcgagccgc cctactggca ctaccccgtc cgccaatcgc tgggctccgc gctgctccgg
      421 ctgggcgatg ctgacggggc agcggcggcc ttcaccgagg atctcggcaa gtttccgaag
      481 aacggctggt ccctgcacgg gctggccgaa gcccgccgcg ctctgggaaa gccaaccgac
      541 gaactggagg cggcccaccg ggaagcgtgg caatatgccg atattgagcc aacggcaggc
      601 gccgagtagc gagttcaaaa ccggaggttc gtgatgtccg aagccctagc accgatcgat
      661 ctgaccacag cggacccgta ctcggtgccc ctggatcaga tcgacatgag ccaggccgcc
      721 ctcttccaga ccaacacctt cggcccgtac ttcgagcgcc tgcgccggga agatccggtg
      781 cactacaccg cggacagttt tttcgggcct tactggtcca tcacccgctt cgacgacatc
      841 gtctacgtgg acaagcacca cgagctcttc tcgtccgagg acggcatcac cctggccgac
      901 cgccagcagg actttgccac cgtcaacttc atcgccatgg atccgcccaa gcacgacgat
      961 cagcgaaagg ccgtccaggg cgtcgtggcg ccgcgcaacc tggccaagat ggaagcgctc
     1021 atccgcggac gcgtcgccga cattctcgat tcgctgccgc tgggtgagac cttcaactgg
     1081 gtcgaccggg tttccatcga gctcaccacc cagatgctgg ccacgctgtt cgacttcccc
     1141 ttcgaggagc ggcgcaagct cacctactgg tcggacatgg ccacgtccag cgagctggcc
     1201 ggcggcccaa cgcccgagcc agtgcggcgt gaggcgctga tggaatgtct ggaagccttc
     1261 acccgaatct ggcacgagcg cgcaagccag ccgccggacg agagcgtgga tctgatctcc
     1321 atgctcgccc acagcgacga cacgaaggac atgctcaatc ggccgatgga atacctcggc
     1381 aacctgatcc tgctgatcgt gggcggcaac gacaccacgc gcaactcgat aaccggcggc
     1441 gtgctcgcgc tgaaccagaa tcccggcgag tacgacaagc tgcgccagca tcccgagctc
     1501 attcccaaca tggtggcaga gatcatccgc tggcagacgc cgctggccta catgcgccgg
     1561 acggccaccc aggacacgga gctcggcggc aagctgatcc gcaagggcga caagatcgcc
     1621 atgtggtacg tctccggaaa ccgcgacgag acggccatcg accagcccga ggagttcatc
     1681 atcgatcggc ccaacgcccg ccatcacctt tccttcggct tcggcattca ccgctgcatg
     1741 ggcaaccgcc tggcggaaat gcagctgcgc atcgtctggg aggagatcct caaacgcttc
     1801 cacacggttg aggtggtggg cgaaccggtg cgcgttcagt ccagcttcgt caagggatac
     1861 tcgcagctgc ccgtgcgact gcatccggtc tagtttgcgc gcggcccctc ggaacggcgg
     1921 gtgtcagggg gctcagcctg gggtgcccgt tgcggttatc cgccgtcgag cagcaccgtg
     1981 cggatgtcgt tggcgacggt atccgcatga aggaaagaga tgctgtagat gttgcgtatc
     2041 tgcctgtccc ggtcgatgag gtagacgcgc agaatgtgcg acagcgtgcc aacgaactcc
     2101 ccgtcggcgt ctatgtcgcg caccacccac tgatcgtacg catccagcac cgaggtgaga
     2161 tccgctgcgt tcgcaaccgt gacgaagcgc cagtcgaacc ccggcttgcg gaagtggccg
     2221 ccgtactgtc gcaggaccgc cggcgtgtcg tgggacggat cgaagctgaa gcttacgagc
     2281 cgcaccttac cctggaggtc aggggcgctg gtgatggccc cctgcagctt gccgagcacg
     2341 tgggtcgcca ggggacagcc gttgacgtcg ttgcaacggg tgtagatgaa gctcagcacg
     2401 accaccttgt cgccgagcat ttcgtgcagc cgtccgataa ctccctgggc atcgaccacc
     2461 ctggcatcgg ccacttcccc caggggcggc agcgcgtagc cgcccacggg gggcggggcg
     2521 aagtccagat cggcgtatcc cggcgcaagc accaccggcg cggcatgctg gtgcgaagcg
     2581 ccggagtcag gctgcgcgtg agctccaccg gcggtgaaag ccaccgccag cgtgctcaac
     2641 aggcaggcac gccatcccac cggtcaatgc agtcccacct gctgcggcgg gcgtttgccg
     2701 tagagcgcgt atgagccgaa gcgcatctgg tgcggcgcgc ccagcccggc cgccacgaaa
     2761 tcaatcgtga accccggcgt catcttctcg ccatcccagc cgtagagctt gaagtactgc
     2821 aggtccggac cgctgggtcc cgtcttgtcc cagttggcca gcagcgagga ggtgaagtag
     2881 agccgctcgc cgtcccagct ctgggacacc atgttcacct gctctccgat cttctgctcg
     2941 aacagctgct tcggcgcgtg gggatcggag atgtcgtaga ggtgcaccag accgtcattc
     3001 caggtattga cccagaggag gttgtcttcc gcagagatcg aaatgtccac cggcagcggg
     3061 atcttggcag catcgccgat atccgctacg gccttggcct gccactcgcc gccctcg
//
LOCUS       DBNHKV010000368         2195 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1540546, whole genome shotgun sequence.
ACCESSION   DBNHKV010000368 DBNHKV010000000
VERSION     DBNHKV010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1540546"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="AC16PP_11785"
     CDS             complement(<1..543)
                     /locus_tag="AC16PP_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482354.1"
                     /translation="MTGLFLRALLAWLAVCPALVEGAEAVSPETATAGEPGRPLPMES
                     DGKRNPLTPDVRDDDTELTMQRGDFVIAPIPISDPVLGEGLVLAAAYFYPQTEEQAAS
                     QPASVTAVAGMYTSSESYAYGIGQQNYWGEDRWRFSGALAHADLQLKLRPGADNGDGG
                     RTDWLLDGNVLQAELSRMIAG"
     gene            775..2112
                     /locus_tag="AC16PP_11790"
     CDS             775..2112
                     /locus_tag="AC16PP_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482355.1"
                     /translation="MNIRNVSLALILAAVCVPSQATDTEEQQEFDSPLILKASEVLSA
                     ETRNGEHHEVREEIVNDGFMNTYVIDSPFGTFEAYGSSMLRTRINEIGALAELDGLSK
                     TKVFMEAAAQSAVRAVTAPVSAAATIVTNPIGTVTGLPGGLARMFNRQKRKVEGAVAT
                     TASAAKSATGADDSGEEASIAEQAGEGAVDLGKSLMGTTRAERRWAKKLGTDPYTSNE
                     TLRKGIADVAVVDSSGYFLAELAVPIGGIVDLVVDVNEAIWERDAYELRQMNLVKLQD
                     MGVAEEEATAFLDNVAISPTIQTLIINGLGLLETTAGRDAFLRQATLAEDEAESRFYL
                     QSLAMIAWLNQEGENVAAITVHGPVSIAVDTGGNRVAMLPVDHLTWNPTTVEAVGLWP
                     AQDPDVPSDGRVYISGDISALARSHLEQAGWSARSKIARDFFTYLSSQESDEP"
BASE COUNT          436 a          727 c          681 g          351 t
ORIGIN      
        1 tcccgcgatc atgcgtgaaa gctctgcctg caggacgttg ccatcgagca gccaatcggt
       61 ccgccccccg tcgccgttat ccgcgccggg gcgaagcttc agctgcaagt cggcatgagc
      121 cagggcgccg ctgaagcgcc atctgtcctc gccccagtag ttctgctggc cgataccgta
      181 agcgtagctc tcgctgctgg tatacattcc cgccaccgcg gtgacggaag caggctggga
      241 agctgcctgc tcctccgtct ggggatagaa ataggcggcc gcgagaacca gaccctctcc
      301 aagcaccggg tccgaaatcg gaatgggggc aatcacgaag tcgccccgct gcatcgtcag
      361 ctcggtatcg tcgtcgcgaa catcgggcgt tagagggttc ctctttccat ccgattccat
      421 cggcagtggc cggccgggct cgcccgccgt cgccgtttcc ggggagaccg cttcggcccc
      481 ctccaccagc gcaggacaca cagcgagcca agcgagcagc gcgcgcagga ataagcccgt
      541 catccaatcg tcacctcggg cagcaacgtc cgccaccctg ccggccccgt cgtcgcctga
      601 cactgaccat aagctttccc ggaagagccc tcgcgatggc gcgagccaca tgatttccct
      661 ggcggatgca gccctcgatc cacccgacta agatgtgaag accccgcagc gatgttcccg
      721 gcgagggacc tgccggcatc agagccggtt ctcgacaacg cccaaggatt ttctatgaat
      781 atacgtaacg tctcgctggc gctcattcta gccgcggtgt gcgtgccatc ccaagccacc
      841 gacaccgagg agcagcagga attcgattcc cccttgattc tgaaggccag cgaagtgctc
      901 agcgccgaga cccgcaacgg agagcaccac gaggtacgcg aggaaatcgt caacgatggc
      961 ttcatgaaca cctacgtcat cgactcgcct ttcgggacat ttgaagccta cggctcgtcc
     1021 atgcttcgaa cgcgcatcaa cgagataggc gcgctggccg agctggacgg cctgtcgaaa
     1081 accaaagttt tcatggaagc agctgcccag tcggcggtaa gggccgtcac cgcccccgtc
     1141 agcgccgccg cgaccatcgt cacgaacccc atcggcaccg tcaccgggct ccccgggggc
     1201 ctggcacgca tgttcaacag gcagaaacgc aaagtcgagg gcgcggtggc cacgacggcg
     1261 tcggcagcaa agtcggcgac gggcgccgac gactccgggg aggaggccag catcgcggag
     1321 caggcaggcg agggggccgt cgatctcggc aaaagcctga tgggaaccac ccgcgcggag
     1381 cggcgctggg ccaagaagct gggtaccgat ccatatacct ccaacgaaac tctcagaaag
     1441 ggaatcgccg acgtggccgt cgtcgacagc agcggctatt tcctggccga gctggcggtg
     1501 cccatcggag gcatcgtcga tctggtggtg gacgtcaacg aggccatctg ggaacgcgac
     1561 gcctacgagt tgcgccagat gaacctggtc aagctgcagg acatgggtgt ggcggaggag
     1621 gaggcgaccg cgtttttaga taacgtcgcc atcagcccca ccattcagac cctgatcatc
     1681 aacggtctgg ggctgctcga aaccactgcc gggcgggacg ccttcctgcg tcaggcgacc
     1741 ctggccgagg acgaggccga atcccgattc tacctccaga gcctggccat gatcgcgtgg
     1801 ttgaaccagg agggggagaa cgttgctgcc atcaccgtgc acggacccgt ctcgatcgcc
     1861 gtcgacaccg gcggcaaccg tgtcgccatg cttcccgttg accacctgac gtggaacccg
     1921 accaccgtcg aggccgtcgg cctgtggccg gcacaggacc ccgacgtccc ttcggacggc
     1981 cgggtctaca tttccggaga catctctgcc ctggcgagaa gccatctgga gcaagcaggc
     2041 tggtcggcac gatccaagat tgcccgggat ttcttcacgt atttgagcag ccaggagtca
     2101 gacgagccgt agcccgccgg tttcgaacgg gagagcgtct gcggaaagga cctggcgctt
     2161 caggagaagc agcgggcgac cgccgaatac ggccg
//
LOCUS       DBNHKV010000369        10028 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1469716, whole genome shotgun sequence.
ACCESSION   DBNHKV010000369 DBNHKV010000000
VERSION     DBNHKV010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10028
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1469716"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="AC16PP_11795"
     CDS             <1..355
                     /locus_tag="AC16PP_11795"
                     /inference="COORDINATES: protein motif:HMM:NF024375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6482356.1"
                     /translation="GPHYHLGAADFMVLSGHIGYRAGPPEGYGPGVWFYEPAGARHEA
                     TQRIGEEDLIYTANVYGPLQFDEGPDTPIAAVLSWMQYKAMADAGGVDLVHSTFDNDA
                     SLLAWAPIGSEGARL"
     gene            396..947
                     /locus_tag="AC16PP_11800"
     CDS             396..947
                     /locus_tag="AC16PP_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482357.1"
                     /translation="MDVNKGIRVCEEIAASETDPRKRHNLEMVIAHMRAEARCDVQGV
                     LDTLVDEPCYVWHSAPDDPLLNPRGSREAVADFYDRMIVRTGAHRLEWDITRVMVDEH
                     AVLTEGVMRVAFPGWALAEMDIAVDDPDAYYVAEGITTVIWPVDPETGLLVGEEVYEY
                     DKMAGIADRKIAPSDILPLSSAA"
     gene            975..1427
                     /locus_tag="AC16PP_11805"
     CDS             975..1427
                     /locus_tag="AC16PP_11805"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6482358.1"
                     /translation="MTDPVTELRDAIDRQTIADLQNRYAYAIDAGRYDDLDGVFTPDA
                     VADYGPAGRVEGVEAIKATCRHALEPLTAAQHINTNHWAVIEGDHARAGCYLHVHQHR
                     ESTPGGDHLEMGGRYEDALIRTPDGWRITSRKLTILWSQGNPEVRWER"
     gene            complement(1424..2347)
                     /locus_tag="AC16PP_11810"
     CDS             complement(1424..2347)
                     /locus_tag="AC16PP_11810"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR6482359.1"
                     /translation="MRQALLETNLGLPNRRSGKVRDLYDVTLDGGEEALLIIATDRIS
                     AFDVVMANGLPGKGIVLTQISRFWFDHFAGEVDHHLISTDVADVPGLSDAQREALAGR
                     IMLCRKTRVVPIECIARGYITGSGWKDYQRTGAVCGIELPEGLRNSDRLEEPLFTPST
                     KADTGHDENISFEEGVGIVGRELMDWLGDTTLRLYAKARDYALERGIILADTKFEFGQ
                     LPGREEPILIDEIFTPDSSRFWPADEWEPGREQPSFDKQIVRNYLETVVAAGEWDKTP
                     PGPALPDEVVEKSIGRYLEAYQRLTGSTLDL"
     gene            complement(2384..3151)
                     /locus_tag="AC16PP_11815"
     CDS             complement(2384..3151)
                     /locus_tag="AC16PP_11815"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6482360.1"
                     /translation="MGRLKGKVAVITGGAGGIGAAAGRLFAEEGADVLLVDVDEKALE
                     ETVRGIGSNRVSYFVGDVTASADNAAMVACAEERYGGVDVLIANAGIEGDVAVPIVDY
                     DEARFDQVLGVNVKGVFLGLKSAIPAMARRGGGSIVITSSVAGVGGSPGMSAYVTSKH
                     AVIGLMRSAARECAGQNIRVNTVNPSPVETRMMRSIEEGIAPGGGEQVKSLMQSRIAL
                     QRYAEPEEIAKVMLFLASDDSAWVTGSVYMADGGMTA"
     gene            complement(3178..4401)
                     /locus_tag="AC16PP_11820"
     CDS             complement(3178..4401)
                     /locus_tag="AC16PP_11820"
                     /inference="COORDINATES: protein motif:HMM:NF004609.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HIR6482361.1"
                     /translation="MKGFTTKIVHSDRLEVIEHGSLHKPVHSTVAYGYDDARDLAAVF
                     QGTKAGYTYGRQVNPTVEALQTKISTMEQGLQTVCFSTGMAAIGTMLFALLRNGDHFV
                     SSSFLFGNTNSLFNSFSTHGVDVTFVDATDVDAVAAGINERTRLVFVETIANPRTQVS
                     ALEEIGKLCAERGLIYVVDNTMTSPYLFLPASVGASLVVNSLTKYIGGHGNALGGAVT
                     ELGNFDWADFSNIYDTYKSGAAEKWAITQIKKKGLRDFGAALAPEAAHHIAVGSETLG
                     LRLERQCANAVAMTAMLDAHPKVKAVYYPGLKQHPQHERASRLFRHPGALFSFELVPE
                     VDIWNFMNALDVIIKSSNLGDNRTLAIPVAHTIYYEMGPERRASMGIDDSLIRISVGI
                     EESEDLLGDLERALG"
     gene            complement(4420..6618)
                     /locus_tag="AC16PP_11825"
     CDS             complement(4420..6618)
                     /locus_tag="AC16PP_11825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014871164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR6482362.1"
                     /translation="MLNRKSAGSGLVGASILLVDDKPELLNSLYQLMVLHGYEPDKAL
                     GGQQALDLLAEKSYDVVLLDLIMPGVSGHDVLDYAARNELTCKIIVVSGDSSFSGVKH
                     ALHCGAFDFVKKPYEAGELIATMETALRQCQLEAQNELMEEKVKESEELHRYIVNSSP
                     DLVYMLDRNGCFTFLNDRVESLLGFSRDELIGKHYTELVDDDHLELARNVFNERRTGD
                     RAVSNAEMRLKSRHARRGARLFHAHAIWTELTAMGVYSDPKERTRENFVGTYGIARDI
                     SERKEAQEVISFQAYHDLLTHLPNRALLKDRLSLAITQARRSKRRLAVMFLDLDRFKI
                     VNDTLGHSMGDRLLKAVANRLQSCLRRGDTLSRFGGDEFTLLLPEVRTRDDVVVIATK
                     ILDRLGGPFVIDGLELFVGASIGVAMYPEAGETEEALIQNADIAMYHVKGRGKNGYQF
                     FSEEMNHKYSTRLSMERELRNGLTQEELVVYYQPQVALKTGEIVGVEALVRWRHPERG
                     LVEPGDFLPMAEETGLITQIDTFVQRRACTDVAGWERAGLGSIRLSVNLSAPQIEQED
                     FVDNFVADMAAAGLDPSRVKLEITENILMQDMEVVIPKLKQLRQLGVRIAIDDFGTGY
                     SSLSYLQQFPIHTLKIDRSFVGDIRADHGDASIVNAIVAMARGLNLDLIAEGVENRTQ
                     LRYLRSQGCSEVQGFIFSDPVPADQVKLMLQQNPFRAMVLDEQPEAATAV"
     gene            complement(6749..7336)
                     /locus_tag="AC16PP_11830"
     CDS             complement(6749..7336)
                     /locus_tag="AC16PP_11830"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6482363.1"
                     /translation="MSVDSELELRWERLGSEYGNDLILFKTRFDRMRNPRNDAVLRRL
                     VLESVDWVNVVALTADGLSVMVRQFRFGVDYTTLETPGGMVDPGEDSLAAARRELLEE
                     TGYGGGDWTYLGAVEPNPAFHDHLCHHWLASGVSLLQEPAPTPGEAIEVTLMSERAVA
                     DAVQQGVIKHALALSALSRVFELWPRPFRHERDPS"
     gene            complement(7333..8457)
                     /locus_tag="AC16PP_11835"
     CDS             complement(7333..8457)
                     /locus_tag="AC16PP_11835"
                     /inference="COORDINATES: protein motif:HMM:NF018805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HIR6482364.1"
                     /translation="MRIVTLPALLLTLLIVAPGCQPDEPAGGAMREGTLRLGAVLGET
                     DAQGFARAEAVVPFAFPGDHGPHPRFRSEWWYLTANVQNEFGAEFGVQFTVFRQALAP
                     GPGPANPWLSNQVFLAHAAVTGVLEQGHWHEERVARGHPGVAGATAEPFRVWVDGWEL
                     AGRNGGFDEQRLSVKADAFAVDLQLDALKRPVLQGDRGLSAKGPEQASYYYSVPRLSA
                     RGTLRIGEVEHQVSGRAWIDREWSTSALSSGQVGWDWFALHLDDSWEIMVYQLRRADG
                     SRDRYDHAVLIDPYGAKHPLGADDFELEPEIFWQDDDGVAWPLQWRLKALDRELRIRA
                     AVPDQRMETSVSYWEGLVGVFDMAGRRTGRGYMELTGYEG"
     gene            complement(8464..>10028)
                     /locus_tag="AC16PP_11840"
     CDS             complement(8464..>10028)
                     /locus_tag="AC16PP_11840"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6482365.1"
                     /translation="QIAIIWHRRRRLLNDRLEAMGVSRIELTRGFVRAFFLLGLVASF
                     AGLLGGVVLARGLGAMTTLAADAAGQWPPITGWLLLKAGVSGMGVGSLSAWMACRREW
                     GSGSGAPTWILAGVLLAVAAAGVYFPVTGLFGGFAAVLALGMLVVAVVQPMLEALRAS
                     ALRLRGRLLNRHALREVAWYPRDLSVAVGALSLAVATSMAVGLMVESFRTDFAQMLDQ
                     RLAHDLYVDGGGRDLADVHGWLLEQPSVDHVQPYGRARHRVGTVPIELGYSPLDEAEA
                     ARYGVRGGLGPGEALVSERLAGLLGADVGDRLALGGGLRIAGVFSGFGEAGYRVLVDE
                     SVAADLGIELVHDRLSVSLAADDQVAGADLDARLLAAFPGVEVEPRGRMRQVAMEIFD
                     RTFAITSSLTLLALLVAAIGVHNSLVALRLNQQQGVHLLVMLGVTAGELARVAVRRAL
                     GIGSVAVALALPLGVLMAWLLCAVINPRAFGWTVDLHLALSDVAVPLALGIGAAVLAG
                     LLPSPRDSAGEI"
BASE COUNT         1807 a         3433 c         3133 g         1655 t
ORIGIN      
        1 cgggccccac taccacctgg gcgccgccga cttcatggtg ctcagcggtc atatcggtta
       61 tcgggcaggg ccgcccgagg gctacgggcc gggggtctgg ttctacgagc ccgccggagc
      121 gcgtcacgaa gccacccagc gcatcggcga ggaagatctc atctacaccg caaacgtcta
      181 cggcccgctg cagttcgacg agggccccga cacgcccatc gcggccgtgc tgtcctggat
      241 gcagtacaag gcaatggccg atgccggcgg ggtagatctc gtgcacagca ccttcgacaa
      301 cgacgcctcg ctgctcgcct gggcgcccat cggcagcgag ggcgcccggc tctgagccgg
      361 gcttcggcgg aagcaagcgg gggatcgacg tgcggatgga cgtgaacaaa ggcatccggg
      421 tctgcgagga aatcgcagcc tcggaaaccg accccaggaa gcgccacaat ctggagatgg
      481 tgattgccca catgcgggcg gaagctcgct gtgacgtcca gggcgttctc gacacgctcg
      541 tcgacgagcc atgctacgtc tggcacagcg cgcccgacga tccgttgctc aacccacgcg
      601 gcagcaggga ggcggtcgcc gacttctacg atcggatgat cgtcaggacc ggcgcccatc
      661 ggctcgaatg ggatatcacc cgggtcatgg tggacgagca cgccgtgctc accgaaggcg
      721 tcatgcgggt cgcgtttccc ggctgggcgc tggcggagat ggacatcgcc gtcgacgacc
      781 cggacgccta ctacgtggcg gagggcatca ccacggtcat ctggcccgtc gacccggaaa
      841 ccgggctgct cgtcggagaa gaggtctacg agtacgacaa gatggccggg atcgccgatc
      901 ggaagatcgc gccgagcgac atcctgccgc tgtcctcggc ggcgtaacgt ccgggcaatg
      961 acacgcatgg caccatgacc gatcccgtca cggaactgcg cgatgccatc gatcggcaga
     1021 ccatcgccga tctgcagaac cgctacgcct atgccatcga cgccggtcgc tacgacgacc
     1081 tggacggggt gttcactccc gatgccgtgg ccgactacgg ccccgcggga cgggtcgagg
     1141 gcgtggaagc gatcaaggcg acctgccgcc acgctttaga gccgctgacg gccgcgcagc
     1201 acatcaacac caaccactgg gccgtcatcg aaggtgacca cgcgcgggcc gggtgctacc
     1261 tgcacgtcca tcagcaccgg gagagcacgc cgggcggcga tcatctggag atgggcggcc
     1321 gctacgagga cgcgctgatc aggacgccgg acggctggcg catcacctcg cgcaagctga
     1381 ccatcctctg gagccagggc aaccctgagg tgcgctggga gcgctagaga tcgagcgtcg
     1441 agcccgtgag acgctggtag gcttcgagat agcgcccgat gctcttctcc acgacctcat
     1501 cgggcaacgc gggtccaggg ggcgttttgt cccactcccc cgccgccacc accgtctcca
     1561 ggtaattgcg gacgatctgc ttgtcgaagc tgggctgctc gcgtccgggt tcccactcgt
     1621 cggcaggcca gaacctcgag ctgtcgggcg tgaatatctc gtcgatcaga atcggttcct
     1681 cccgacccgg cagctgaccg aactcgaatt tcgtgtcggc gagaatgatg ccgcgctcaa
     1741 gcgcgtagtc gcgcgccttg gcgtacaacc tcagggtcgt gtcgccgagc cagtccatga
     1801 gctcgcggcc gacgatgccg acgccttcct cgaagctgat gttctcgtcg tgaccggtgt
     1861 ccgccttggt ggatggcgtg aacagcggct cttccagccg atcgctgttt ctcaacccct
     1921 cgggcagctc gattccgcac accgcacccg tgcgctgata atccttccag ccgctgccgg
     1981 tgatgtagcc gcgcgcgatg cattcgatgg ggaccacgcg ggtcttgcgg cacagcatga
     2041 tgcgtcctgc gagggcctct ctctgggcgt cgctcagccc gggcacgtct gccacgtcgg
     2101 tggaaatcag atggtggtcg acctctccgg cgaagtggtc gaaccagaac cgtgagatct
     2161 gggtgagaac gatgcctttg ccgggcaggc cgttggccat cacgacgtcg aacgcgctga
     2221 tccgatcagt ggcgatgatc agcagagcct cctcgccgcc gtcgagggtg acgtcgtaga
     2281 gatcgcgaac cttgcctgag cgtcggttcg gcaaaccgag attcgtttcc agtagcgcct
     2341 gtctcatttt tcccccatgt ccaatcgagt caaactttca atgtcaggcc gtcatcccgc
     2401 cgtccgccat gtagaccgag ccggtgaccc aagcgctgtc gtcgctggca agaaagagca
     2461 tgaccttggc gatctcctcc ggctcggcgt atcgctgcag cgcgatgcgc gactgcatca
     2521 ggctcttcac ctgctcgccg ccccccggcg cgatgccctc ctcgatgctt cgcatcatac
     2581 gcgtctccac gggagatgga ttcacagtgt tgacgcggat gttctgcccg gcgcattccc
     2641 gggcggccga tcgcatcagc ccgatcaccg cgtgcttgct ggtgacgtaa gcggacatgc
     2701 ccggagatcc tccgacgccg gcaaccgagg aggtgatgac gatgctgccg ccgccacgcc
     2761 gcgccatggc ggggatggcc gatttcagcc ccaggaagac gcccttgacg ttgacgccca
     2821 ggacctgatc gaacctcgcc tcgtcgtagt ccacgatggg cacggcaacg tcgccttcga
     2881 tccccgcatt ggcaatgagc acgtccaccc cgccgtagcg ctcttccgcg cacgcgacca
     2941 tggcggcgtt gtccgcagaa gcggtgacgt caccgacaaa gtagctgacg cggttgctgc
     3001 cgataccgcg cacggtttcc tccagcgcct tctcgtccac atcgaccagc aggacgtcgg
     3061 cgccttcctc ggcgaaaagc cgacccgcag cagcaccgat gccaccggcg ccccccgtga
     3121 tgacggccac tttacccttc agtcttccca tgccttgcgc ttcccccgca caccgattca
     3181 gcccagcgcg cgctcgagat cccccaggag atcctcgctc tcctcgatgc ccacggatat
     3241 gcggatcagc gaatcgtcta tgcccatgct cgcccgccgc tccggaccca tctcgtagta
     3301 aatcgtgtga gccacgggaa tcgccagggt ccggttgtca cccaggttgc tggacttgat
     3361 gatcacatcc aacgcgttca tgaagttcca gatgtccacc tcgggaacca gctcgaagct
     3421 gaacagcgcg cccggatggc ggaacaggcg gctcgcccgc tcgtgctggg gatgctgctt
     3481 aaggccggga tagtagacgg ctttgacctt cgggtgcgca tcgagcatcg ccgtcatggc
     3541 gaccgcgttg gcacactggc gctcaagccg caacccaagg gtctccgagc ccacggcgat
     3601 gtgatgagcg gcttccgggg caagcgcggc gccgaagtcc cgcagtcctt tcttcttgat
     3661 ctgggtgatg gcccacttct cggcggcccc ggatttgtac gtatcgtaga tgttggaaaa
     3721 atccgcccag tcgaaattcc cgagctccgt caccgctccg cccagcgcgt tgccgtgacc
     3781 gccgatgtac ttggtcaggc tgttcaccac caggctggcg ccgacgctgg ccggcagaaa
     3841 caggtagggc gacgtcatgg tgttgtccac cacgtaaatg aggccgcgct cggcgcacag
     3901 cttaccgatc tcctccaacg cagatacctg cgtgcgcggg ttggcaatgg tttccacgaa
     3961 cacgagccgg gtacgctcgt tgatgccggc ggccacggcg tccacatcgg tggcatcgac
     4021 aaacgtgacg tccaccccgt gggtcgaaaa ggagttgaac aggctgttgg tgttaccgaa
     4081 caggaaggag ctcgacacga agtgatcgcc gttgcgaagc agggcgaaga gcatggtccc
     4141 gatggcagcc atgcccgtgg agaaacagac ggtctgcagc ccctgttcca tggtggagat
     4201 cttggtctgg agggcttcca cggtggggtt cacctgccgg ccataggtgt agccggcttt
     4261 ggtgccctgg aacaccgccg caaggtcgcg ggcgtcgtcg tacccgtatg ccaccgtgga
     4321 gtgcaccggc ttgtgcagcg agccgtgctc gatgacctcg agccggtcgc tgtgcacgat
     4381 cttcgtggtg aaacccttca tcggtagcag cgcgccgtgc tagacggctg tggcggcttc
     4441 tggctgctcg tccaggacca tggcccggaa cggattctgc tggagcatca gcttgacctg
     4501 atctgccggc accgggtcac tgaatatgaa gccctgcacc tcgctgcagc cctgggaacg
     4561 cagatagcga agctgagtgc ggttctccac gccctcggca atgaggtcca ggttgagacc
     4621 ccgcgccatg gcgacgatgg cgttgacgat ggaggcatcg ccatggtctg cgcggatgtc
     4681 gcccacgaag ctgcggtcga tcttcagcgt gtggatgggg aactgctgca ggtagctcag
     4741 cgacgagtat ccggtgccga aatcgtcgat ggcgatccgc accccgagct gccgcagctg
     4801 cttgagcttg ggaatgacca cctccatgtc ctgcatcagg atgttctcgg tgatctccag
     4861 cttgaccctg gagggatcca ggccggcagc agccatgtcc gcaacgaaat tatcgacgaa
     4921 gtcctcctgc tcgatctgcg gggcagacag gttcacggac agccggatac ttccaagccc
     4981 cgcgcgctcc cagccggcaa catcggtgca cgcccggcgc tgaacgaagg tgtcgatctg
     5041 ggtgatcaga ccggtttctt cggccatggg caggaagtcg ccgggctcta ccagcccgcg
     5101 ctccggatgc cgccaccgga cgagcgcctc gacaccgacg atctccccgg tcttcagcgc
     5161 cacctgaggc tggtagtaca cgaccagctc ttcctgggta agcccgttgc gaagctcgcg
     5221 ctccatggac agccgggtcg agtacttgtg gttcatctcc tcggagaaga actggtagcc
     5281 gttcttgccg cgacccttga cgtggtacat ggcaatgtcg gcgttctgaa tcaacgcttc
     5341 ctcggtctcc ccggcctccg ggtacatggc tacgccgatg ctggcaccca cgaacagctc
     5401 gaggccatca atgacgaagg gaccgcccag ccggtcgagg atcttggtgg cgatgaccac
     5461 cacgtcgtcg cgcgtgcgca cctccggcag cagcagcgtg aactcgtcgc cgccgaagcg
     5521 cgacagcgtg tcgccgcgcc gcagacagct ctgcaggcga ttggctaccg ctttgagaag
     5581 ccgatcgccc atgctgtggc ccagggtgtc attgacgatc ttgaagcggt ccagatccag
     5641 gaacatcacg gccagtcttc gcttgctccg ccgtgcctgg gtgattgcca gcgacaggcg
     5701 gtccttgagc agagcgcggt tcggcagatg ggtgagcaga tcgtggtagg cctgaaagct
     5761 gatgacctcc tgggcctctt tgcgttcgct gatatcccgc gcgatgccgt aggttccaac
     5821 gaagttttcc cgggtacgct ccttgggatc ggaataaacg cccatggccg tgagctcagt
     5881 ccagatggcg tgggcatgaa acagacgcgc gccccgtcgg gcgtggcggc tcttcaggcg
     5941 catctccgcg ttggacaccg ccctgtcgcc cgtgcgccgc tcgttgaaca cgttccgcgc
     6001 aagctccagg tggtcgtcgt ccaccagctc cgtgtagtgc ttgccgatga gttcgtcacg
     6061 ggagaatccc agcagacttt cgacacggtc gttgagaaag gtgaagcagc cgttgcggtc
     6121 gagcatgtag accagatccg gggagctgtt gacgatgtag cgatgcagct cctctgattc
     6181 cttgaccttc tcttccatga gctcgttttg agcctcgagc tggcactgtc gcagcgccgt
     6241 ctccatggtc gcgatgagct cgcccgcctc atagggcttc ttgacgaagt cgaaggcccc
     6301 gcaatgcagc gcgtgcttga cgccgctgaa gctggaatcg ccgctgacga cgatgatttt
     6361 gcaggtgagc tcgttgcggg ccgcgtagtc gaggacgtcg tgcccgctca cgcccggcat
     6421 gatcaggtcc agcagaacga cgtcgtagct tttctccgcc agaagatcca gcgcctgctg
     6481 gccgccgagg gctttgtccg gctcgtagcc gtgcagcacc atcagctggt aaaggctgtt
     6541 gagcagctcg ggtttgtcat cgacgaggag aatcgacgca cccaccagcc cggacccggc
     6601 gctttttcga ttgagcaatc gggagaatga ctctcgggga ctggaagcgt ccgccgtgct
     6661 gctttcactc ataccagaac tgtcccacgt cgtgctcgac atgcttacca attgtgacga
     6721 atttttgctg ctctggatag gcgcccgctc atgacggatc acgctcgtgc cgaaacgggc
     6781 gcggccagag ctcgaacacg cgcgacagcg cggaaagcgc cagcgcgtgc ttgatcaccc
     6841 cttgctgcac cgcgtcggcg accgcccgct cgctcatcag cgtcacctcg atggcttcgc
     6901 ccggggtcgg cgccggctcc tgcagcaacg aaacgccgga ggccagccag tgatggcaga
     6961 ggtggtcgtg aaaagcggga ttcggctcaa cggcgccgag gtaggtccaa tccccaccgc
     7021 cgtaaccggt ctcctccagg agctcccgcc gggcggcggc gagcgagtcc tcgcccgggt
     7081 ccaccatgcc tcccggagtc tccaaagtcg tgtaatccac gccgaagcgg aactggcgaa
     7141 ccatgacgga gagcccatcg gccgtaagcg ctacgacgtt cacccagtcc acggactcca
     7201 gaaccagccg ccggagcacc gcatcgttgc gcggattgcg catcctgtcg aaacgggtct
     7261 tgaacagaat caggtcattg ccgtactccg agcccagacg ctcccagcgc agttccagct
     7321 ccgaatccac actcatcctt cgtaccccgt caattccatg tagccgcggc cagtgcgtct
     7381 gcccgccatg tcgaagacgc ccaccagccc ttcccagtag gaaacgctgg tctccatgcg
     7441 ctgatcggga acggcggcgc ggatgcgcag ctcgcgatcg agcgccttca ggcgccactg
     7501 cagcggccag gcaacgccgt cgtcgtcctg ccagaagatc tccggctcga gctcgaagtc
     7561 gtcggcgcca agaggatgct tcgcgccgta ggggtcgatg agcacggcgt gatcgtagcg
     7621 atcccgggac ccatccgcac gcctgagctg gtagaccatg atctcccagc tgtcgtcgag
     7681 gtgaagggcg aaccagtccc aacccacctg cccggacgag agcgcgctgg tgctccactc
     7741 gcggtcgatc caggcccggc cgctgacctg atgctctacc tcaccgatcc tgagcgtgcc
     7801 ccgcgcactt agccgcggca ccgagtagta ataggaagcc tgctccggcc ccttggcgct
     7861 caggccgcga tcaccctgca gaacgggcct tttcagcgca tccagctgca ggtcgacggc
     7921 gaacgcgtca gccttcacgc tcaacctctg ctcgtcgaag ccgccgttcc ggccggcaag
     7981 ctcccagccg tccacccaga cgcgaaatgg ctctgcggtg gcaccggcga cgcccgggtg
     8041 gccacgcgcc acgcgctcct catgccagtg gccctgctcc aggacgccgg taaccgctgc
     8101 gtgcgccaga aagacctggt tggacagcca cgggttggct ggcccgggcc cgggcgccag
     8161 cgcctggcga aacacggtga actgcacccc gaactccgcg ccgaactcgt tctggacgtt
     8221 ggcggtcagg taccaccact cactcctgaa cctcgggtga gggccgtggt ccccagggaa
     8281 cgcgaacggc acgacagcct ccgcccgggc aaaaccctgc gcgtccgtct cgccaagcac
     8341 ggcgccgagc cgcagagtcc cttccctcat ggcgccgccc gccggctcgt ccggttggca
     8401 accgggggcc acgatcagca gcgtcagcag caacgcgggc agggttacga tgcgcattcc
     8461 ctatcagatc tccccggccg aatcccgcgg cgacggcaac agtcccgcca gcacggcagc
     8521 accgatcccg agcgcgagtg gcacggccac gtcgctcaat gccagatgca gatccaccgt
     8581 ccagccgaag gcccgcgggt tgatcaccgc gcacaacagc catgccatga gcacccccag
     8641 gggcagggcc aacgctaccg cgacgctgcc gatgcccagc gcccggcgca cggccacccg
     8701 ggcgagctcg cccgcggtga cgcccagcat gacgagcagg tgcacgccct gctgctgatt
     8761 gaggcgcagg gcgacgaggg agttgtgcac gccgatggcg gccaccagca gggcgagcag
     8821 ggtcagggag gacgtaatgg caaacgtgcg atcgaagatc tccatcgcca cctgacgcat
     8881 ccgcccgcgg ggctccacct ccacgccggg gaaggccgca aggagacgcg cgtcgagatc
     8941 ggcaccagcg acctgatcat ccgccgccag cgagacgctg agccggtcgt gcacgagctc
     9001 gatgcccagg tcggccgcaa ccgactcatc cacgagcaca cggtaaccgg cctcgccaaa
     9061 accgctgaaa acgcccgcga tccggaggcc cccgcccaga gccagacgat cgcccacatc
     9121 tgctccgagc aagccggcca gccgctcgct gaccagcgct tcccctgggc ccagccctcc
     9181 gcgaacgccg tatcgggccg cctccgcctc gtcgagcggc gagtagccaa gctcgatggg
     9241 cacggtgccg acgcgatgtc tggccctccc gtagggctgc acgtgatcga ccgacggctg
     9301 ctctaagagc caaccgtgca cgtcggccaa gtcgcgcccg cccccatcca catacagatc
     9361 gtgggcgagg cgctgatcga gcatctgcgc gaagtccgtg cggaagctct ccaccatcag
     9421 ccccacggcc atgctagtgg ccacggccag cgacagcgca ccaacggcca cggacagatc
     9481 ccgcgggtac cacgccacct cgcgcagagc gtgccggttg aggagacggc cacgcaagcg
     9541 cagcgcagag gcccgcaacg cctccagcat gggctggacg acggcaacca ccagcattcc
     9601 cagcgcgagc acggctgcga agccgccgaa cagacccgtc accgggaagt agacgccggc
     9661 cgcggcgacc gcgagcagca cgccagccag gatccaggtg ggcgccccgg agcccgatcc
     9721 ccactcgcgg cggcaggcca tccacgcgct cagcgagcca acgcccatgc ccgacacgcc
     9781 ggctttgagc aggagccaac ccgtaatcgg cggccactgc ccggccgcgt ctgcagccag
     9841 ggtcgtcatg gcaccaagcc cgcgggccag cacaacgccg ccgagcaacc cggcgaagct
     9901 cgccaccaag cccagcagga aaaacgcgcg cacgaaccca cgggtcagct cgatcctgga
     9961 cacgcccatg gcttccagcc ggtcgttgag caggcgacgg cgacggtgcc agatgatggc
    10021 tatctggt
//
LOCUS       DBNHKV010000370         3538 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1396926, whole genome shotgun sequence.
ACCESSION   DBNHKV010000370 DBNHKV010000000
VERSION     DBNHKV010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1396926"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..657
                     /gene="nqrE"
                     /locus_tag="AC16PP_11845"
     CDS             49..657
                     /gene="nqrE"
                     /locus_tag="AC16PP_11845"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HIR6482366.1"
                     /translation="MEQYLGLFVRAVFVENMALTFFLGMCTFIAISKKIATAIGLGVA
                     VIVVLAITTPVNNLIFNYLLRDGALAWAGFPDVDLSFLGFLSYIGVIAAMVQILEMFL
                     DRFVPVLYNALGVFLPLITVNCAILGASLFMVERDYTFAESVVFGLGAGVGWALAITA
                     LAGVREKLKYSDVPEGLRGLGITFISVGLISLCFMSFGGIDI"
     gene            668..1897
                     /gene="nqrF"
                     /locus_tag="AC16PP_11850"
     CDS             668..1897
                     /gene="nqrF"
                     /locus_tag="AC16PP_11850"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HIR6482367.1"
                     /translation="MLDTTIILGVVMFTVVVLFLVMVILFARSRLVSTGEITIEINED
                     PENTIVVPAGGKLLSTLASKGIFLASACGGGGTCAQCRCQVLDGGGSILSTEEGHFTR
                     GEIRDHFRLSCQVAVKQDMKIHIPEDAIGVQRWECEVTSNPNVATFIKELNLKLPEGA
                     NVDFRAGGYVQLEIPPYEMDYKDIDVEDEYQEDWDRFDVWKYHSKVTEPTIRAYSMAN
                     YPEEKGILKFNIRIASPPPGMDVPPGNMSSYVFGLKPGDKLTVFGPYGDFFVKDTPAE
                     KIWIGGGAGMAPLRSQIFDELKRRKQSYTMSYWYGARSLREMFYTEEFDDLASEYDNF
                     EWHVALSDPLPEDEWTGHTGFIHQVVYDNYLKDHPNPEDCEYYLCGPPPMVAAVLQML
                     EDLGVEPENILLDDFGG"
     gene            complement(1947..2573)
                     /locus_tag="AC16PP_11855"
     CDS             complement(1947..2573)
                     /locus_tag="AC16PP_11855"
                     /inference="COORDINATES: protein motif:HMM:NF022426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HIR6482368.1"
                     /translation="MPRTLATILLAGTAALGGCASLSESQCLAGDWQTVGYRDGSSGQ
                     NRAALLEHQNACVKHGVTPDREAYLAGWDQGILQYCQPRNGFALGERGAAFPTLCPGA
                     VVDPFEAAYHDGRRLYLARAEIRRLEREINAREHRLEHLDEAIASAESRLVEDGLSSA
                     QRRELLEETKHMSREQGEIEVELKELRIDVALEHQRLAQLRHALASAS"
     gene            complement(2712..3113)
                     /locus_tag="AC16PP_11860"
     CDS             complement(2712..3113)
                     /locus_tag="AC16PP_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482369.1"
                     /translation="MESRGIPTICMSSAYSITAAVNPPRAVYLDFPLGHTAGKAGDKA
                     LQRRVMIDTLSALDGILAPGTIRELPYEWSSDDAWKDQAMRPKEGSRPSDDRVERHDT
                     PQYQTAEDRDAAEAQLATGQCPGCVWLSETT"
     gene            complement(3153..>3538)
                     /locus_tag="AC16PP_11865"
     CDS             complement(3153..>3538)
                     /locus_tag="AC16PP_11865"
                     /inference="COORDINATES: protein motif:HMM:NF019003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HIR6482370.1"
                     /translation="QPLAKPLADCRVGLIASGGIYRHGQVAFHFRDDLSFREIPRDTE
                     VSELRATHFAYDLTDARKDPNVVFPMETLRDLARTGRIRELSANAYTFMGGIYSARKV
                     RDILAPAIASRLQKDEVDVAILVPV"
BASE COUNT          623 a         1066 c         1143 g          706 t
ORIGIN      
        1 cgagtacgaa atcgcgccga acgttcagta tggggaggcc atctgatgct ggaacagtat
       61 ctggggcttt tcgttcgcgc ggtcttcgtc gagaacatgg ccctgacgtt ttttctaggc
      121 atgtgcacgt tcatcgccat ctccaaaaag atcgcgacgg caatcggcct cggcgttgcc
      181 gtcatcgtgg tgcttgccat cacgacgccg gtcaacaacc tgatcttcaa ctatcttctg
      241 cgtgacggag cgctggcctg ggccggtttc cccgacgtgg acttgagctt tctcggcttt
      301 ctctcgtaca tcggcgtgat cgcggccatg gtgcagatac tggaaatgtt cctggaccgt
      361 ttcgtgcccg tgctctacaa cgcgctcggc gtgtttctgc cgctgatcac ggtgaactgt
      421 gccattctcg gagcgtccct gttcatggtg gagcgcgact acacctttgc cgaaagcgtc
      481 gttttcggcc ttggggccgg cgtcgggtgg gcgcttgcca tcacggcgct cgccggcgtt
      541 cgggagaagc tcaagtacag cgacgtgccg gaggggctgc gtggtctggg tatcacattc
      601 atctccgtgg ggctgatcag tctgtgcttc atgtccttcg gtggcatcga catttaagga
      661 cggttagatg ttagacacga cgatcatcct tggtgtggtc atgttcacgg tggttgtcct
      721 tttcctggtc atggtgatcc tgttcgcccg ctcgcgcctg gtcagcaccg gcgagatcac
      781 catagagatc aacgaggatc ctgagaacac catcgtggtg ccggcgggcg gcaagctgct
      841 cagcaccctg gcctccaagg ggattttcct cgccagcgcc tgcggtggcg gcgggacctg
      901 cgcccagtgt cgttgccagg ttcttgatgg tggcgggagc atcctctcca ccgaggaggg
      961 tcacttcacc cgcggcgaga ttcgtgatca cttccggctt tcgtgtcagg tggcggtgaa
     1021 gcaggacatg aaaattcaca ttccggagga cgccatcggc gtccagcgat gggagtgtga
     1081 agtgacctcg aaccccaacg tggcgacctt catcaaagag ctcaacctga agctcccgga
     1141 gggcgccaac gttgatttcc gagccggcgg ttacgtccag ctggaaattc cgccttacga
     1201 gatggactac aaggacatcg acgtggagga cgagtaccag gaggactggg atcggttcga
     1261 cgtctggaag tatcacagca aggtgaccga acccaccatc cgggcatact ccatggccaa
     1321 ttacccggaa gagaagggga tcctaaagtt caacattcgt attgcctcgc cgccgcccgg
     1381 gatggacgtc ccgcccggca atatgtcttc ctacgtcttc gggctcaagc ccggggacaa
     1441 gctcacggta ttcgggccct acggtgactt cttcgtcaaa gacacgcctg ccgagaagat
     1501 ctggatcggc gggggcgccg gcatggcgcc gctgcgctcg cagattttcg acgagctcaa
     1561 gcggcgcaag cagagctaca cgatgagcta ctggtacgga gcccgatcac tgcgggagat
     1621 gttctacacc gaggagttcg acgatcttgc cagcgagtac gacaacttcg agtggcacgt
     1681 cgccctctcg gatccgttgc ccgaggacga gtggacgggt catacgggat tcatccatca
     1741 ggtggtgtac gacaattacc tcaaggacca tcccaatccg gaagattgcg agtactacct
     1801 ctgcgggcct ccgcccatgg tggcggccgt cctgcagatg ctcgaggacc tcggcgtcga
     1861 gccggagaat attctgctcg acgatttcgg gggttagtcg ggttttgtag cgcgacgcga
     1921 aagcacccgg gcgccgacgt gctggatcag ctcgctgatg cgagggcgtg acgaagctgc
     1981 gcgaggcgct ggtgctcgag agcgacgtcg atgcgcagtt ccttgagctc gacttcgatc
     2041 tcgccctgct cgcgggacat gtgcttggtt tcctccagca gttcccgccg ctgcgcgctg
     2101 ctcaacccat cttccaccag acggctctca gctgacgcga tggcctcatc gaggtgctcg
     2161 aggcgatgct cgcgggcatt gatctcgcgc tcaagccgcc ggatttccgc ccgcgccaga
     2221 tagagccggc gaccgtcgtg gtaggcggct tcaaacgggt cgaccacggc gcccggacaa
     2281 agggtcggaa aggcggcgcc gcgctcacct aaagcaaacc cgttgcgcgg ctggcagtac
     2341 tgaagaattc cctggtccca accggccagg taggcttcgc gatcgggggt caccccatgc
     2401 ttgacgcatg cgttctggtg ctcgagaagc gccgcgcggt tctggccgct tgacccgtcc
     2461 cggtagccga cggtctgcca gtcgccggcg aggcactggc tttcgctcag gctggcgcac
     2521 ccacccaggg cggccgtccc ggcgaggaga atggttgcta gggtccgggg cataggacct
     2581 ccgttgtcac atgcttcctg agggtcagcg gggaaagatg gcccaaacgc gcgagccgga
     2641 ccgccaatca gttcacactt tggcagcccg cccggaggtg cagggcggcc ggaaccgggc
     2701 cgcggtcgga atcaggtcgt ttcggacaac cagacgcagc cggggcattg gccggtggcg
     2761 agctgcgcct cggcggcgtc ccggtcctcg gcggtctgat attgaggcgt atcgtgccgc
     2821 tcgacgcggt cgtccgaagg ccgcgacccc tccttcgggc gcattgcctg gtctttccag
     2881 gcgtcgtcgg acgaccactc gtacggcagc tcgcgaatgg tgccgggggc gagaatgccg
     2941 tccagggccg acagggtgtc gatcatcacg cggcgctgca gggccttgtc gccggccttt
     3001 cctgccgtgt gacccagcgg aaagtccagg tacacggctc tcggcggatt gacggctgcg
     3061 gtgatggagt aggcgctgga catgcagatg gtgggaatgc cccggctttc cacctctctt
     3121 gctatcagcc cgacggactg atggcagacg ggtcagaccg gcaccagaat ggccacgtcc
     3181 acctcatcct tctgcagtct ggaggctatg gccggcgcca gaatatcgcg gaccttacgg
     3241 gccgagtaaa tcccgcccat gaacgtgtag gcattggcgg agagctccct tatgcgtccg
     3301 gttcgggcga gatcgcgaag cgtctccatc ggaaacacga cgttggggtc tttgcgcgcg
     3361 tcggtgagat cgtaggcgaa gtgggtggct cgcagctcgg atacctcggt atcacgaggg
     3421 atttccctga agctcaagtc gtcccggaag tgaaaggcca cctgaccatg ccggtagatg
     3481 ccgcctgagg cgatcaggcc gacccggcaa tctgccaggg gctttgccag cggctgga
//
LOCUS       DBNHKV010000371         4046 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_264249, whole genome shotgun sequence.
ACCESSION   DBNHKV010000371 DBNHKV010000000
VERSION     DBNHKV010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_264249"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1440
                     /gene="rpsA"
                     /locus_tag="AC16PP_11870"
     CDS             <1..1440
                     /gene="rpsA"
                     /locus_tag="AC16PP_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR6482371.1"
                     /translation="TRLSREKAKRAESWMMLEDAFNKGEVITGAINGKVKGGFTVDIS
                     DIRAFLPGSLVDIRPLRETTHLEGKPLEFKVIKLDQKRNNVVVSRRAVLEQENSAERE
                     ALLESLQEGQEVKGIVKNLTDYGAFVDLGGVDGLLHITDMAWRRIRHPSEMVNVGDEV
                     NVKILKFDREKNRVSLGMKQLGDDPWVELTRRYPEGSRVRATVTNLTDYGCFAEIEEG
                     VEGLVHVSEMDWTNKNIHPSKVVNVGDEVEVMVLDIDEERRRISLGIKQCRANPWEEF
                     SAHHAKSDRIKGSIKSITDFGIFIGLDGGIDGLVHLSDISWNEPGETAVRRYSKGEEI
                     ETVILSVDPERERISLGIKQLDEDPFTDYTAVNDRGSVVTGVIVEVEPKEAVIRLAED
                     VTGVLKASEISIDRIEDARNVLTAGEEVEVKIISVDRKNRNLALSIKAKAVDDEREAV
                     REHRRQESERVGPSTLGDLIKAQMDQEES"
     gene            1561..1887
                     /gene="ihfB"
                     /locus_tag="AC16PP_11875"
     CDS             1561..1887
                     /gene="ihfB"
                     /locus_tag="AC16PP_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HIR6482372.1"
                     /translation="MTKSELIERIAARQTQLSAKDVELAVKTLIEQMSETLAAGHRIE
                     IRGFGSFSLHYRAPRIGRNPKTGESVGLAGKYVPHFKPGKELRDRVNQGLERERAEQE
                     SAGLPS"
     gene            1897..2190
                     /locus_tag="AC16PP_11880"
     CDS             1897..2190
                     /locus_tag="AC16PP_11880"
                     /inference="COORDINATES: protein motif:HMM:NF018058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HIR6482373.1"
                     /translation="MAWLSRFFWALLALLVFCLAGLAVNQEPISLKFVTWQTPQLSVF
                     WWLLAAFSTGVLLGLVGISVVRARLGLKNRSLVKRLEASERELRRLRSVSYHE"
     gene            2197..3369
                     /gene="lapB"
                     /locus_tag="AC16PP_11885"
     CDS             2197..3369
                     /gene="lapB"
                     /locus_tag="AC16PP_11885"
                     /inference="COORDINATES: protein motif:HMM:NF008757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HIR6482374.1"
                     /translation="MPENLVVYLLLVLALGIGFLLGRRERKRKQSSPGMVAQEYFRGL
                     NHLLNERHDLAIDTFVEAMAVDSDTVDTHLALGSLVRRRGEVDRAIRIHQNLLARPVL
                     SPAHRAQTELELARDYLSAGLLDRAENLLLELVKKPGDDRDVARTMLLEIYQREREWE
                     RAVEVARELSRADRSYRTPLAHFQCELAEKALADGDLRRARADLERAGDFDPGCARVA
                     LVGAKVASAGGRHRDVKRLLRKARSLEPELSGETVAMFRAASEELGDRADFVRYLREC
                     LERAAPLEVVQALTREIEADEGDEVAREFLIEQLLRNPNLAGFVSLLEQLDRGGAALE
                     ADQLALVRRFSSSLLQKQPSHRCKDCGFSGHTLIWQCPSCKTWGSIKPIVRYDGDV"
     gene            3495..3776
                     /locus_tag="AC16PP_11890"
     CDS             3495..3776
                     /locus_tag="AC16PP_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIR6482375.1"
                     /translation="MTMTIKRAMGAALLLAGVLAGPVLAAPESVNINSANAETLAEVL
                     NGVGLTKAQAIVEYREQHGAFRDAYELANIKGIGERTVEKNEARIRLQD"
BASE COUNT          775 a         1205 c         1376 g          690 t
ORIGIN      
        1 acccgcctgt ccagggagaa agccaagcgc gcggaatcct ggatgatgct cgaggacgcc
       61 ttcaacaagg gcgaggtcat caccggtgcc atcaacggca aggtcaaggg tgggttcacg
      121 gtggacatca gcgacatccg ggcgttcctt cccgggtccc tggtggacat caggccgctg
      181 cgcgagacca cccatctcga gggcaagccc ctcgagttca aggtgatcaa gctcgaccag
      241 aagcggaaca acgtcgtggt gtcacggcga gccgtgctag agcaggagaa cagtgccgag
      301 cgcgaggcgc tcctggaatc cctgcaggaa ggtcaggaag tcaaaggcat cgtgaagaac
      361 ctcacggact acggcgcgtt cgtggacctc ggcggcgtag acgggctgct gcacatcacc
      421 gacatggcct ggcgccggat tcgacatccc agcgaaatgg tcaacgtagg cgacgaggtc
      481 aacgtcaaga ttctgaagtt cgacagggag aagaaccgcg tcagtctcgg catgaagcag
      541 ctgggggacg atccgtgggt ggagctgacc cggcgctatc cggaaggttc gcgggtccgg
      601 gccacggtca ccaaccttac cgattacggc tgttttgccg agatcgagga aggtgtcgag
      661 ggtctcgtgc acgtttccga gatggattgg accaacaaga acatccaccc ctccaaggtc
      721 gtcaacgtgg gcgatgaggt cgaggtgatg gtcctggaca tcgacgagga acgccgtcgc
      781 atctcgctgg gcatcaagca gtgccgtgcg aacccgtggg aggagttcag cgcccaccac
      841 gccaagagtg atcggatcaa gggcagcatc aagtccatca ccgacttcgg gatcttcatc
      901 ggtctggatg gcggcatcga tggtctggtg cacctttccg acatctcctg gaacgagccc
      961 ggcgaaaccg ccgtgcgtcg ttacagcaag ggtgaggaga tcgagacggt cattctgtcc
     1021 gtcgatcccg agcgtgagcg aatctccctg ggcatcaagc agctcgacga ggatccgttc
     1081 accgactaca ccgcggtcaa cgatcgcggc tcggtggtca cgggcgtgat cgtggaagtc
     1141 gaacccaagg aagccgtcat tcgcttagcc gaagacgtga ccggtgttct caaggcgtcg
     1201 gagatcagca tcgaccgcat agaggatgct cgcaacgtgc tgacggcggg tgaagaggtg
     1261 gaggtcaaga tcatcagcgt ggaccgcaag aaccgcaatc ttgccctgtc aatcaaggcc
     1321 aaagcggtgg acgacgagcg cgaggcggtg agggagcacc ggcgccagga aagcgagcgc
     1381 gtgggaccga gcacgcttgg agatctcatc aaggcgcaga tggatcagga agagagctag
     1441 ccttcaaaaa gagcgggcgg cccgatcaaa ggaccgcccg tgacaacgcg atgaacgggg
     1501 gagggggtcc ggaagacgcc ggtcaatcgg gcgtaggtcg ggcctgacaa gcaggcagac
     1561 atgaccaaat cggaactgat cgagcgaatc gcggccaggc agacgcagct gtctgcgaag
     1621 gacgtcgagc tcgccgtaaa gacgctcatc gagcagatgt ctgaaacgct tgctgcggga
     1681 caccggatcg agatcagagg cttcggcagc ttttcgctgc actaccgggc gccgcgaatc
     1741 ggccgtaatc caaagaccgg cgagtccgtg gggctggcgg gcaagtacgt tccccacttc
     1801 aagccgggca aggagctgcg agatcgggtg aaccagggtc tggagcggga acgagcggag
     1861 caggagtccg ccgggcttcc gagctagcct aagcccatgg cttggttgtc acgattcttc
     1921 tgggcgctgc ttgcgctgct ggtgttctgc ctggcagggc tggcggtcaa tcaggaaccg
     1981 atttcgctca agttcgtcac ctggcagacc ccgcaactaa gcgtattctg gtggctgcta
     2041 gccgcgttca gcaccggtgt gctgttgggt ctggttggca tctcggtcgt tcgggcccgg
     2101 ctcgggctga agaaccggtc gctggttaaa cggctcgagg caagcgaacg tgagctgcgc
     2161 agattgcgca gcgtgtccta ccacgagtaa acgcgcatgc ccgagaacct tgtcgtctac
     2221 ctgcttctcg tgctggcact cggcatcggc tttcttctcg ggcgacgcga gcgcaaacgc
     2281 aaacagtcca gtcccggcat ggtggctcag gagtacttcc ggggcctgaa ccatctcctc
     2341 aacgagcgtc atgacctggc catcgacacg ttcgtggaag ccatggcagt ggacagcgac
     2401 accgtcgaca cccacctggc gctcggaagt ctcgtccggc gacgtggaga ggtggaccga
     2461 gccatccgca ttcaccagaa cctgctcgcc cggccggtcc tttctcctgc gcaccgcgct
     2521 cagaccgagc tcgagctggc gcgcgactat ctgtccgccg ggctgctgga ccgggcggaa
     2581 aatctgctgc tggaactggt caagaaaccc ggcgacgatc gcgacgtggc gcgcacgatg
     2641 ctgctcgaga tctatcagcg ggagcgggag tgggagcgcg ccgtggaggt ggcccgggag
     2701 ctctcccgtg ccgatcgatc ctatcgcacg ccgctggctc actttcagtg cgaacttgcg
     2761 gagaaggctc tggccgatgg ggatctgcgg cgcgcccgcg ccgatctcga gcgagccggg
     2821 gactttgatc cgggttgcgc tcgggtcgcc ctggtcggcg cgaaagtcgc ctccgccggt
     2881 ggccgccacc gcgacgtcaa gcggctgctg cggaaggctc gcagcctcga gcccgagctc
     2941 agcggggaaa ccgtggcgat gttccgggcc gccagtgaag agctcgggga tcgtgccgat
     3001 ttcgtgcgct atctgaggga gtgtctggag agggcggccc cgctggaggt cgttcaggcg
     3061 ctcacccggg agatcgaggc tgacgaaggc gacgaggtgg cgcgggagtt tctgatcgag
     3121 cagctgctgc gcaaccccaa tctggcgggc ttcgtctccc tgctcgagca gctcgaccgt
     3181 ggcggagcgg ctctcgaggc cgaccagctg gcgctggttc ggaggttctc gagctcgctg
     3241 ctccagaagc agccgagcca tcgctgcaag gactgtggct tcagcgggca cacgctcatc
     3301 tggcagtgtc cgagctgcaa gacctggggc agcatcaagc ccatcgtgcg ttacgacggc
     3361 gacgtctgaa ctccattggc ccgcgtcctc gccgggtccg aacttacgga aacggcggct
     3421 ctttgcggct tacgcgggcg cctgggcgcc tcgtagcatt gtcgtcctcc tcaccactga
     3481 taacggagat acccatgacg atgacgatca aacgtgcgat gggcgctgcg ctgctgctgg
     3541 ccggtgtgct cgccgggccg gtgctcgcgg ccccggagag tgtgaacatc aacagcgcga
     3601 atgcggagac gcttgccgag gtgctgaacg gcgttgggct caccaaggcc caggccatcg
     3661 tcgagtaccg tgagcagcac ggcgcgttcc gcgacgcata tgagcttgcg aacatcaagg
     3721 gcatcggcga acggaccgtc gagaagaacg aggcgcggat ccgcctgcag gattgaccct
     3781 gcggcttgag gcaccttcga tgcggcgttc gccgggcgtc gtcctgagtt cgccaggcca
     3841 ggctccgggt ggcaggcttg ccccggagcc tggccgctcg tcggtgtttt tgtgacgccg
     3901 gtcaagcgca ttgtccggag cacctctcat tgccggggcg cgggcggtaa gatggagcac
     3961 gaaagccgcc gatggcgtcg cgacgaagta tggattcttt gcagcgtttg cccctaatgc
     4021 ccacctctgc cggcgcgggt accggc
//
LOCUS       DBNHKV010000372         4588 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1754724, whole genome shotgun sequence.
ACCESSION   DBNHKV010000372 DBNHKV010000000
VERSION     DBNHKV010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1754724"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="AC16PP_11895"
     CDS             complement(<1..330)
                     /locus_tag="AC16PP_11895"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6482376.1"
                     /translation="MSFSFTDEQEQFREATRRFLQDKSPTTEVRRLMETSEGFDEAVW
                     KQLSGELALPGIHIPEQYGGAGFGPVELGIIMEELGRSLLCAPYFGSSVLAATAILEA
                     ATEDWKCE"
     gene            complement(349..1533)
                     /locus_tag="AC16PP_11900"
     CDS             complement(349..1533)
                     /locus_tag="AC16PP_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6482377.1"
                     /translation="MSELSEFRNQTRAWLETSCPAGARGPGQIPWGSSKIPLESDVRL
                     WLERMAERGWTVPTWPKEYGGAELSRDQYMVLIDELKRINARTPLTGRGVNYIGPTIL
                     EFGTHEQKERWLPGIARGEGGWAMGYSEPGAGSDLASLSTRAELQGDQYVLNGRKTWT
                     SDAMDCDYIFVLARTDPDRPKHQGISLLLVDMNQPGVQVRPIRLLSGASPFNETTFEN
                     AVARADDVIGEVNAGWTVGKRLLQFERSTHAGINISGSQGGRSAESNLAERLKQFVPS
                     RDGKLQDPELRERLVRYEQNGHAQRLTQKRVIEELKARAPGYASSAVKLTGALHTQEG
                     DELLLAAMGTQGIVWDGPGLERSDSEALRTWLRQKSLTIAGGTKEVQLNIIAKRVLGL
                     PD"
     gene            1618..2391
                     /locus_tag="AC16PP_11905"
     CDS             1618..2391
                     /locus_tag="AC16PP_11905"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR6482378.1"
                     /translation="MKIATWNVNGLNARQEFVGLWLAHRQPDLVGLQEIKLVDEAFPA
                     DFFAGLGYQVLTHGQKAWNGVAVLSRLPVEIVQKGLPGQESAGSRLITVRVDDRLEFT
                     TVYCPNGKTLDHEDYAKKLAWFTSLSEYWRDRPSAGAVLCGDFNVVPEAVDSWRGEAG
                     DGRIFHTLEERAAFRSLLDAGLEDLFRMAKPDEQAFSWWDYRGGSFHRGHGLRIDLIL
                     GSRDVAERVVDVRTDRDYRKKKDGLTASDHAPVIAEIDL"
     gene            2551..3102
                     /locus_tag="AC16PP_11910"
     CDS             2551..3102
                     /locus_tag="AC16PP_11910"
                     /inference="COORDINATES: protein motif:HMM:NF039725.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6316 family protein"
                     /protein_id="HIR6482379.1"
                     /translation="MLPEWFRSDEIFSDEGRWYFGARGGLHVGPYGDRELASARSLSV
                     SRRLRALRTDADRLRYVRKVLHEEWESLGVSASAAEAETTIDLSPPSEPVRSGERQRK
                     WCRSSRFFQIHGVWFVSTREGINIGPYGTQFEARKHERHLIALLVRARDPAEARRVIY
                     RFKHRPNLTGTPWSQTDEVARTG"
     gene            complement(3126..4304)
                     /locus_tag="AC16PP_11915"
     CDS             complement(3126..4304)
                     /locus_tag="AC16PP_11915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015270460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6482380.1"
                     /translation="MNQPDTALSPKVSELVARTTEFVTDKVIPYEKDPRNGSHGPSDA
                     LRVELQTAAREAGLLVPQLAEHLGGHDLNHREMAAVFRAAGYSPLGPIALNIMAPDEG
                     NMHLLHKVATPEQQERFLQPLAAGTIRSAFLMTEPDGGAGSDPGMLATMAEREGDDWV
                     INGRKSFITGGEGAGFGIIMAKTGSSATMFLADMNTPGISIERVLDTIDSSMPGGHAV
                     VRLDGVRVPADQILGELDEGFRYAQVRLAPARLTHCMRWWGSAQRAHDIASDYACRRQ
                     AFGKPIIDHEGVGFMLADNLMDLKQAELMIDWCAGVLDSGAPGTAESSMAKVAVSEAL
                     FRVADRCVQVLGGTGVTQDTIVEQVFREIRAFRIYDGPSEVHRWSLAKRIKRERLAST
                     "
     gene            complement(4301..>4588)
                     /locus_tag="AC16PP_11920"
     CDS             complement(4301..>4588)
                     /locus_tag="AC16PP_11920"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6482381.1"
                     /translation="HGGDPWTDLCVKLLLKWPNLYYSTSAWAPKHYPRNVLEFANKRG
                     RGKVLYAGYFPGLSYERIFTEMDDLPLTDDTWPAFLRDNAINVYKLQDILE"
BASE COUNT          756 a         1486 c         1499 g          847 t
ORIGIN      
        1 ctcgcacttc caatcttccg tagcggcctc gagtatggcg gttgcggcca gcacgctgga
       61 gccgaagtac ggcgcgcaca ggagggaccg accgagctcc tccatgatga tgccgagctc
      121 cacgggaccg aatccggcgc cgccgtactg ctccggaatg tgaatacccg gcagcgccag
      181 ctccccggac agctgcttcc ataccgcctc gtcgaaaccc tcagaggtct ccatcaggcg
      241 cctgacctcg gtggtcgggg acttgtcctg cagaaatcgc cgggtggctt cccggaactg
      301 ctcctgctcg tcggtaaagc tgaaggacat ctcgcaaact cctcgaggtc aatccggcag
      361 gccgagaacg cgtttcgcga tgatgttcag ctgcacttct ttggtcccgc cggcaatggt
      421 cagggatttc tggcgcagcc aggtgcgcag tgcctcgcta tcgcttcgtt cgagtcctgg
      481 cccatcccag acgatcccct gggtccccat ggcggcgagc aggagctcgt ccccttcctg
      541 ggtgtgcagt gcgcccgtca gcttcacagc ggaagaggca taccccggcg cgcgcgcttt
      601 tagctcctcg atgacgcgct tctgggtcag gcgctgggcg tggccgttct gctcgtagcg
      661 aaccagccgc tcgcgcagct cgggatcctg cagcttgccg tcccgactgg gaacgaactg
      721 cttgaggcgt tctgccagat tgctttcggc gctgcggccg ccctgggagc cggagatgtt
      781 gatacctgcg tgggtggaac gttcgaactg cagcagacgt ttccccactg tccagccggc
      841 gttcacctcg ccaatgacgt cgtcggccct ggccacggcg ttctcgaacg tggtctcgtt
      901 gaagggtgag gcgcccgaaa gcaggcgaat ggggcggacc tgcacgccgg gctggttcat
      961 gtccacgaga aggagactga ttccctggtg tttcgggcgg tcgggatccg tgcgggcgag
     1021 aacgaagatg tagtcgcagt ccatggcgtc ggatgtccac gtcttgcggc cgttgagaac
     1081 atactgatcg ccctgaagct ccgcccgggt gctcaggctg gccaggtcgg atccggctcc
     1141 gggctcggag taccccatgg cccatccgcc ttctcccctg gcgattcccg gcagccagcg
     1201 ttctttctgc tcgtgcgtac cgaattcgag tatggtcgga ccgatgtagt tcacgccccg
     1261 tccggtcagc ggcgtgcggg cgttgatgcg tttgagctca tcgatcagca ccatgtactg
     1321 atccctgctc agctcggcgc caccgtactc cttcggccag gtcggaaccg tccagccgcg
     1381 ttccgccatg cgctcaagcc acaggcgcac gtcgctttcc agcgggatct tgctgctgcc
     1441 ccacggtatc tggcccggcc cgcgggcgcc ggcggggcag ctggtttcca gccatgcccg
     1501 cgtctggttg cggaactcgc tcagctcgct catctggttt ctctcaagcg ctcgatccag
     1561 cgatgcacgc cggttgaaac ggcgatcacg gcgaccgatt aacgctacaa ttcatccatg
     1621 aaaattgcca cctggaatgt caacgggctg aacgcccgac aggagttcgt cggtttgtgg
     1681 ctggcccacc ggcagcccga cctggtcggg ctgcaggaga tcaagctggt cgacgaagcc
     1741 ttccccgccg atttcttcgc cgggctgggt tatcaggtcc tcacccatgg ccagaaggca
     1801 tggaacggcg tcgcggtgct cagccgcctg cccgtggaaa tcgtccagaa gggactgcca
     1861 ggccaggagt cggcaggatc ccgcctgatc accgtgcggg tcgacgatcg cctggagttc
     1921 acgaccgtct actgccccaa cggcaagacg ctcgaccacg aggactacgc gaagaagctt
     1981 gcctggttca cctcgctcag cgagtactgg cgtgatcgcc cctcggcagg ggccgtcctc
     2041 tgcggagact tcaacgtggt gccggaagcc gtcgacagct ggcggggcga agccggcgac
     2101 ggcaggattt ttcacacgct cgaagagcgt gcggccttcc ggtcgctgct cgatgcgggg
     2161 cttgaggacc tgtttcgtat ggccaagccg gacgagcagg cgttttcgtg gtgggactac
     2221 cgcggcgggt cgtttcaccg cggccacggg ctgagaatcg acctgatact gggctcgcgc
     2281 gacgtggccg agcgcgtggt cgacgtccgt accgatcggg actatcgcaa gaagaaggac
     2341 ggactgaccg cctcggatca cgcccccgtc atcgccgaga tcgaccttta aaagattggt
     2401 cgccccgctt tcgggactgg cccagaggcc gcaggctgga caccgcttgg ttaaatggtt
     2461 cacggtttga gggcccgaaa ggggatatcg tctcccagca ggcagacctg ggatcaggct
     2521 taccggcaga tttattggcg gatgcacgga atgcttccag agtggttcag atcagacgag
     2581 atcttctccg acgaggggcg gtggtacttc ggtgcccggg gcgggttgca cgtcggtcct
     2641 tatggggacc gcgagctcgc cagcgcccgc agcctctccg tctcgcgccg cttacgggcg
     2701 ctcagaaccg acgccgatcg gctcaggtac gtccgcaagg tgctccacga ggagtgggag
     2761 agcctggggg tatccgccag cgctgcggag gcggaaacga ccatcgatct ctctcctccc
     2821 agcgagcccg tccgtagcgg tgagcgccag cggaagtggt gccgttcgag ccgtttcttc
     2881 cagatccacg gcgtgtggtt cgtgtccacc cgggagggca tcaacatcgg gccctacgga
     2941 acgcagttcg aggccaggaa gcacgagcgc cacctcatcg cgcttctcgt cagggcgaga
     3001 gatcccgcgg aggccagacg ggtgatctat cgcttcaagc accgaccaaa cctgacgggt
     3061 actccctggt ctcaaaccga cgaggtagcc cgcaccggct gaacgcggct tcacggcggc
     3121 agctctcaag tcgatgcgag ccgctcccgc ttgatccgct tggccagaga ccagcggtga
     3181 acctcggagg ggccatcgta gatccgaaag gctctgatct ccctgaaaac ctgctccacg
     3241 atggtgtcct gggtcacccc ggtgccgccc agcacctgca cgcagcggtc ggcgacacgg
     3301 aacagcgcct cggagacggc gaccttcgcc atgctcgact cggcggtgcc cggcgcgccc
     3361 gagtcgagca ccccggcgca ccagtcgatc atcagctcgg cctgtttcag gtccatcagg
     3421 ttgtcggcca gcatgaagcc gacaccctcg tggtcgatga ttggcttgcc gaaggcctgg
     3481 cgccggcagg cgtagtccga ggcgatgtcg tgggcccgct gggcgctccc ccaccagcgc
     3541 atgcagtggg tcagacgcgc gggcgccagg cgcacctggg catagcggaa gccctcgtcc
     3601 agctcgccca ggatctggtc cgccggaacc cgcaccccgt cgaggcgcac gacggcgtgg
     3661 cccccgggca tggagctgtc gatggtgtcc aggacgcgct cgatggagat gcccggcgtg
     3721 ttcatgtcgg cgagaaacat ggttgccgac gacccggtct tggccatgat gatgccgaac
     3781 ccggcgcctt cgccgccggt gatgaacgac ttgcggccgt tgatcaccca gtcgtcgccc
     3841 tccctctccg ccatggtggc cagcatgccc gggtctgacc ccgcgccgcc gtcgggctcg
     3901 gtcatcagga acgcggaccg gatggtcccc gccgccagag gttgcaggaa ccgctcctgc
     3961 tgctcggggg ttgccacctt gtgcagcagg tgcatgttgc cctcgtcggg ggccatgatg
     4021 ttcagcgcga tgggccccag cggcgagtac ccggcggccc ggaacacggc agccatctcc
     4081 cggtggttga ggtcatggcc gccgaggtgc tcggccaact ggggcacgag gaggccggcc
     4141 tcacgagccg ccgtctgcag ttcgactcgc agagcatcgc tgggcccgtg tgatccgttg
     4201 cgaggatcct tctcgtaggg aatgactttg tcggtgacga actcggtggt tcgcgcgacc
     4261 agttcagaaa ctttggggct caacgcggtg tccggctggt tcattcgagg atgtcctgaa
     4321 gtttgtagac gttgatggcg ttgtcgcgga gaaatgccgg ccaggtatcg tctgtcagcg
     4381 gcaggtcgtc catctccgtg aagatgcgct cgtagctcag gccggggaaa tagccggcgt
     4441 agagcacctt gccgcggccg cgcttgttgg cgaactcgag aacgttcctg gggtaatgct
     4501 taggcgccca ggcagacgtg gaatagtaga gattcggcca cttcaacagc agcttcacgc
     4561 aaaggtccgt ccatgggtcc ccgccgtg
//
LOCUS       DBNHKV010000373        13082 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1682564, whole genome shotgun sequence.
ACCESSION   DBNHKV010000373 DBNHKV010000000
VERSION     DBNHKV010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13082)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13082)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13082
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1682564"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="AC16PP_11925"
     CDS             <1..1292
                     /locus_tag="AC16PP_11925"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6482382.1"
                     /translation="TTLDELIDSLKDSSALQAKRLQEREARFVAARNERERLLEDARD
                     RRREAEREADRLRADFEGSEQELADLETRLDENAGDLRELFTVVRQITGDVAPMVESS
                     LVSAEQGPRNALLRRLAEPGAIPAAEELRELWMILLDEMHHSGRVSRFEAPVISADGA
                     ERTRRVTRIGTFTALADGSYLRYLPETGRLLALGRQPAGVDVGDVLAFEEAREPLQRV
                     AIDPSRGSILSLIVQTPDVRERIEQGGVIGYIILALGALGLLLGVERLVTIGVARQRT
                     RRVLADSAAAGDHVIVELRELARDEAYLADAEALSAKMDEVVTIASEKLRRGLPTLAI
                     FAAVSPLLGLLGTVTGMIETFQVITLFGAGDPRLMSGGISQALITTQLGLAVAIPLLL
                     IQSFVQGRANALIATLDETAAELFAQGRTHRGSDHAG"
     gene            1282..1821
                     /locus_tag="AC16PP_11930"
     CDS             1282..1821
                     /locus_tag="AC16PP_11930"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6482383.1"
                     /translation="MLGDLFGNSDLIMAGGWIIVLIMALTFVMWMMILDRYVYLWRDR
                     GRAVSRKFALWQQYSSPDPLNNKRLRAALTGQFLEDSRRWIGTIQVITAVLPLLGLLG
                     TVTGMIKTFDVLTAFGTGNIRGMAEGISQALITTMAGLIAALTGIYFANDLEERIATE
                     ADHLAGRFNEGFADRGNGR"
     gene            1818..2228
                     /locus_tag="AC16PP_11935"
     CDS             1818..2228
                     /locus_tag="AC16PP_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR6482384.1"
                     /translation="MKLHTREEGGSAAINLTPLIDMVFILLIFFAVNATFVKITGVSV
                     DQPTAKTTSLQPASTILIAVTDAGEIWIDKKKVDKRRVRGVVERMFIEAPDAAVVVLS
                     DEESRTGLVVEVIDQVRLAGAGRVALAAAEPEEQ"
     gene            2228..2872
                     /locus_tag="AC16PP_11940"
     CDS             2228..2872
                     /locus_tag="AC16PP_11940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6482385.1"
                     /translation="MALRQGFIFLGALAVTLSLYVMMDRMVGRDRTRLLSPVDAQSIE
                     FVRTEMEDRSRTKDRRRKPPPKPQEMKRVRADLQTNLNRAADLPTETLAFNIKSLLGE
                     GGGVALGERLVQGSGAALELVMASDLTPLSMLPPQYPPSALMRGVEGWVDMVFIVTEE
                     GGVVDPTILESRPGTTFDAAAKAAAMRWRFRPVVRNGQPTPIRARIRIDFELPE"
     gene            2869..4128
                     /locus_tag="AC16PP_11945"
     CDS             2869..4128
                     /locus_tag="AC16PP_11945"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6482386.1"
                     /translation="MKGAALTMLVMLALLPASRLAAQDVPSPAFAQQLARVMDRAEED
                     PEWALERLAEMMRANRNRPTELRYAVRQRAAMLMQTERLDLAAEEMLAAVTGKDPGYA
                     PELRFLLGQVWLLKDDARTALGHLELWARHADPLDPAGLFLVGYAHLQLDEFEPAAKA
                     LETAIATSIKPRPQWFEILAYVYTRLDRPADAIGLLETLIASQPQQSRWWRQLGSIYL
                     LIDDVPRGTAGLTVLAELEPLGLSDSRSLARLFASLDMPADAAYVLDAAMTRARTAAE
                     LPGAPSEPDGSEKPEVPQELPGYEDVMLLAELWIMAREFDAAVGTLKTASAMRDDGEA
                     QTLLGQLYLQREDYPQADRHLDAALAAYGEEPPPRLIYLKAVVALNRGDDEAASRALK
                     RISGDEAFAQRVARLQRYIDSRRQRGA"
     gene            4228..4992
                     /gene="modA"
                     /locus_tag="AC16PP_11950"
     CDS             4228..4992
                     /gene="modA"
                     /locus_tag="AC16PP_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6482387.1"
                     /translation="MSSARKRSRLLQLLATCWLLAGAGNAWAERALAAVASNFVVPAE
                     LLAAEFERATGHTVSIAAGATGKLYAQVVQGAPFDLLLAADAWRPERLELEGRAVPGS
                     RFTYAVGLLTLWSPRPDAFGEDPVGYLRAGRLRRLALANPDLAPYGTAARQVLEHLGL
                     QAQLADKMVFAENIGQAHAMVDTGNADAGFLARAQVIGRPGSHWNVPVGMHLPIEQQA
                     VLLRRAADNAAATGFLGFLRTEAARGAMATAGYGLP"
     gene            4993..5664
                     /gene="modB"
                     /locus_tag="AC16PP_11955"
     CDS             4993..5664
                     /gene="modB"
                     /locus_tag="AC16PP_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR6482388.1"
                     /translation="MPDLAPLWLTLHLATATTLILLVIGTPIAWWLARTRSPLRAPVE
                     ALTALPLVLPPTVLGFYLLVMLSPTSPFGSFWVQVTGEALTFSFAGLVVASVIYSLPF
                     MVQPLTNAFSAIGPGPMEAAASLGAGPFDAFRCVAAPLAKRGFVTAAALSFAHTVGEF
                     GVVLMVGGNIPGETKVISIAIYEHVETLRYAEAHLLSAGLVAFSFVVLLTVYGLNRRS
                     PLNVG"
     gene            5654..6760
                     /gene="modC"
                     /locus_tag="AC16PP_11960"
     CDS             5654..6760
                     /gene="modC"
                     /locus_tag="AC16PP_11960"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HIR6482389.1"
                     /translation="MSVDELDIEITASLPGFDLRLKRTLPLQGVTAVFGPSGSGKSTL
                     LRVLAGFIRPASGRVSCGGDVWFDKAANVHLPPHRRPVGMMFQDARLFPHLNVAGNLD
                     FAVRRQRRPGNGIEQPEVVAALDLGPLLTRQVDSLSGGERQRVALGRTLLASPRLLLL
                     DEPLAALDRERKADILPYLDQIQRGFGIPTLYVSHDVDEVAQLCDRVLVLAGGSERAF
                     GATADIVERLDLEPLMGRFEAGVLLEGTVTGHDPRLHVTHVDVGGDVLTLPLLSGVAE
                     GHAVRVRIRARDVALATTQPEGLSIRNVLPGRISRLLIEEASGTAEAFVELRGAHLRS
                     RLTLAAVEDLGLREGMPVYALIKSISFESSAPGG"
     gene            complement(6780..7721)
                     /locus_tag="AC16PP_11965"
     CDS             complement(6780..7721)
                     /locus_tag="AC16PP_11965"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6482390.1"
                     /translation="MSDPEAAVMSIGAVTKATGISTHTLRKWESRYGVIEPLRTPTGR
                     RVFTQSQVDRLILLRDLLAQGHQIGRLSTLADDELEALASATPARSSVPEFGLVTVVG
                     PLISAVLSREALGPAPFRLVTRPATEWLAGGEEGSDQEAQALVVELSTLSEESCGRLL
                     ELRRGVYQRVIVVYGFAARTTLQMLLDGGIVCLKAPVDGQEVLRALAQSEAPGVVAAL
                     ADPTIPAPRFSPDSIARLAAMAPAIRCECPNHIAQLLTDISAFEEYSKLCEQGDPADR
                     VLHARLRLIAANARALFEEAMENVARAEGLELEELDG"
     gene            complement(7758..9242)
                     /locus_tag="AC16PP_11970"
     CDS             complement(7758..9242)
                     /locus_tag="AC16PP_11970"
                     /inference="COORDINATES: protein motif:HMM:NF016158.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HIR6482391.1"
                     /translation="MSKVFLSFGNQLFHPRYLREHADAEFVMVEDEQFCRRYAFHKQK
                     LTFVLSAMRSYAQTLRGQGWRVRYFDLDSAATWRSAVREAVAATSADTLCHFELESPD
                     LTVAIARFAEQLNLKLRVIRTPMFINTLDDFRAHLDKHKTPKLLPFYKEQRLQHRILV
                     NGDGLPVGGRWNFDQFNREKLPRDLNPQEPALLSRAPLVRSVSDLVADRFAGNPGDLD
                     GFWLPVTHAGAQTWLDEFLESRFWNFGAYEDALTRRSPFVYHSGLAPLLNVGLLTPEQ
                     VLEAALDYAGQHNVPINSLEGFVRQLIGWREFVRGVFHHYYEPMQSRNIWNADRRLTD
                     AWYRGTTGIAPLDHVIGKALRLGWAHHIERLMVAANLMNLSGIEPQEVYRWFMEMFVD
                     AYDWVMVPNVFGMGLTSEGGIFTTKPYICGSNYVMKMGDFKRGEWCDVMDGLLWRFVA
                     HHEETLKANHRLAPMVANLPRVVRKRPEIFALADEFIETTTRAA"
     gene            complement(9267..10001)
                     /locus_tag="AC16PP_11975"
     CDS             complement(9267..10001)
                     /locus_tag="AC16PP_11975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6482392.1"
                     /translation="MGKDLSRKVAVVAGAAGGLGEALSRALVAHPDYASVFALSRTRP
                     SSDDGLDWFETDTTSPADLESAASRVAQLTDRVHLLLCCSGVLHGGAGSPHRPEKSLA
                     ELELENLQLAVRVNAFAPLAAIRAFRDLLRHDQRPVAAVLSAMVGSIGDNSLGGWYSY
                     RMSKAALNMGVRNVAIEFGRLRNGPSVVAVHPGTTLTPLSEPFVRGRDPRTPDESARH
                     ILSVVHDLSPEDSGRFFNWDGRELPW"
     gene            complement(10169..10984)
                     /locus_tag="AC16PP_11980"
     CDS             complement(10169..10984)
                     /locus_tag="AC16PP_11980"
                     /inference="COORDINATES: protein motif:HMM:NF021623.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR6482393.1"
                     /translation="MHPITVRKMEFDVPGPEDFEPKWAAGDIVLSYVSTGVSLYVAYL
                     EPFLVKSLRRVLDQISDPELKENVDRFCRQEAQHYMQHERFNEAILQFGYPGLAERFA
                     QLKADFERFQATKDDKWCVGFVEGFEAYTTQTALKAFAARVPDHPKTDRRFGELFKWH
                     LLEEIEHRNVAYDIYEHLYGDQLFRAGMCWIAQTHIWRFILDCMKIMSPADVARFGET
                     YRVSGLTRMAAVAAVAPMFLKTYMPWYSPHKYVIPPEVAELSATLTAAAKSAA"
     gene            complement(11049..11828)
                     /locus_tag="AC16PP_11985"
     CDS             complement(11049..11828)
                     /locus_tag="AC16PP_11985"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6482394.1"
                     /translation="MRLSADEAERYARDGFLIRECAFTEQELSSLRLAAERAADAAWR
                     LSRSGRTYVLDGNRFVDAGHVTVQFEHAAGSETIRVVEPVAELDADLNALVDDARICE
                     PMRDLVGSEEIALWTDKLNLKRPREGSGFGWHQDSPYWIHECRHVDHLPNVMVTLDDA
                     SEANGCFRVVRGSHKRGCLPGRADGTQLGGFYTDPDAFDARDQVPMVVAAGSLVFFSP
                     HSVHGSLPNRSDRPRRALVMTYQPAGHPMLKSGLVRNVMGG"
     gene            complement(11852..13012)
                     /locus_tag="AC16PP_11990"
     CDS             complement(11852..13012)
                     /locus_tag="AC16PP_11990"
                     /inference="COORDINATES: protein motif:HMM:NF024863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR6482395.1"
                     /translation="MGSFTIAEIVPDRVCVRGALDFDHRPAGLSPRRLPAWTRPQLPR
                     EMDVMVRMPSGVRLAFTTDAAAIGLTFLATNMVMPPETRRPIPFDLEIDGALWSVASD
                     RGNTILLDRARPGAFELVKGDPDTVLFEDLPAGEKQCELWLPHNAYVELRELTLDDGA
                     FLAEPALDPRPRWVHYGSSISHCMEADHPGLIWPAVAARRAGASLTNLGFGGQCHLDQ
                     FVARTIREVDADVISIKTGINVINRDSMRERVFTPALHGFLDTVREGKPDTPIILISP
                     IYCPSAESRPGPTFPGRDGRFFTVSGHDEIREGCLTLRRVREIIEEVVEQRVAGGDRA
                     LAYVDGLDLFGAADAEDLPDDLHPNAAGYVRMGERFAPALADVLSSPETGGR"
BASE COUNT         2191 a         4391 c         4329 g         2171 t
ORIGIN      
        1 ccaccaccct ggacgagctc atcgactccc tgaaggactc gtccgccctg caggccaagc
       61 gactgcagga gcgggaggca cggttcgtgg cagcccgcaa cgagcgcgag cgcctgctgg
      121 aggatgcccg cgaccgccgc cgcgaagccg agcgggaagc agaccggctg cgggctgatt
      181 tcgagggctc agagcaggag ctggccgatc tggaaacccg tctcgacgag aacgccggcg
      241 atctccggga gctgttcacg gtggtgcgcc agatcaccgg ggacgtggct ccaatggtcg
      301 aaagctcact ggtctcggca gagcagggtc cgcgcaacgc tctgctgagg aggctggcgg
      361 agcccggagc catccccgcc gccgaagagc tgcgcgagct ctggatgatc ctgctggacg
      421 agatgcatca cagcggccgg gtgtcccggt tcgaagcgcc cgtgatctcc gccgacggcg
      481 cggagcgcac gcgccgcgtc acgcgcatcg gcactttcac cgccctggcc gacggcagct
      541 atctgcgcta cctgccggag accgggcgcc tcctggccct cggccgccaa ccggcggggg
      601 tcgacgtggg cgacgtgctg gcattcgagg aagcccggga accgctgcag agagttgcca
      661 tcgacccttc ccggggctcc attctctcgc tgatcgtgca gacgcccgac gtgcgcgagc
      721 gcatcgagca gggcggcgtc atcggctaca tcatactcgc gctcggcgcg ctgggcctgc
      781 tgctgggcgt ggagcgcctg gtgaccatcg gggtcgccag gcagcggacg cgaagagtgc
      841 tcgcggattc cgctgcggcc ggcgaccacg tgatcgtgga gctcagagag ctggcaaggg
      901 acgaggcgta tctggccgac gccgaggcgc tcagcgcaaa gatggacgag gtggtcacca
      961 ttgccagcga aaagctgcgc cgggggttgc ccaccctcgc gatctttgca gccgtgagcc
     1021 ctcttctggg gctgctgggc acggtgaccg gcatgatcga gacctttcag gtcatcaccc
     1081 tgttcggcgc cggcgatccg cgcctgatgt cgggcggcat atcccaggcc ctgatcacca
     1141 ctcagctggg tctcgccgtc gcgatcccgc tgctgctgat ccagagcttc gtccaggggc
     1201 gggcaaacgc gctgatcgcc accctggatg aaaccgcggc cgagctcttt gcccagggcc
     1261 gcacgcatcg gggcagcgac catgctgggt gatcttttcg gcaacagcga cctgatcatg
     1321 gccggcggct ggatcatcgt gctgatcatg gcgctcacgt tcgtcatgtg gatgatgatc
     1381 ctggatcgct acgtgtacct gtggcgcgac cgcggacgag ccgtctcgcg caaattcgcg
     1441 ctgtggcagc aatactcgag cccggatccc ctgaacaaca agcgcctgcg agccgcgctg
     1501 accgggcagt ttctcgagga ttcccgtcgc tggatcggca ccattcaggt catcaccgcc
     1561 gtgctgccgc tgctgggact cctggggacg gtcaccggca tgatcaagac cttcgacgtg
     1621 ctcaccgcgt tcggcacggg caacattcgc ggcatggccg agggcatatc ccaggcgctg
     1681 atcaccacca tggcggggct gatcgcggcg ctcaccggca tctacttcgc caacgatctg
     1741 gaagagcgca tcgccaccga agcggatcat ctcgccggcc ggttcaacga gggctttgca
     1801 gaccggggga acgggaggtg aagctgcaca cgcgggaaga aggcggcagc gcggccatca
     1861 acctgacgcc gctgatcgac atggtgttca tcctgctcat ctttttcgcg gtgaacgcca
     1921 cgttcgtgaa gatcaccggc gtgtccgtgg accagcccac ggccaagacc acgagcctgc
     1981 agccggcgag cacgatcctc atcgccgtca ccgatgccgg cgagatctgg atcgacaaga
     2041 agaaagtgga caagcggcgc gttcgcggcg tggtggagcg catgttcatc gaggctccgg
     2101 acgcggccgt ggtggtgctg tcggacgagg agtcacgcac gggcctggtc gtggaggtca
     2161 tcgaccaggt gcgcctggcc ggggcaggca gggtggcgct ggcggctgca gagcccgagg
     2221 agcagtgatg gcgctgcgcc agggtttcat cttcctcggc gcgctggccg tcaccctgtc
     2281 gctctacgtc atgatggacc gcatggtggg gcgtgaccgc acccgtctgc tcagccccgt
     2341 ggacgcccag tccatcgagt tcgtccgcac cgagatggag gaccgctcac gtaccaagga
     2401 ccggcggcgc aaaccgccgc caaagcccca ggaaatgaag cgcgttcgcg ccgacctgca
     2461 gaccaacctc aaccgtgcgg ccgacctgcc tacggaaacg ctggcattca acatcaagag
     2521 cctgctgggc gaaggcggcg gggtggcgct gggtgagcgc ctggtccagg gcagcggcgc
     2581 cgctttggag ctggtcatgg ccagcgacct gacgcccttg agcatgctgc cgccccagta
     2641 cccgccttcg gcgctgatgc gcggcgtcga ggggtgggtg gatatggtct tcatcgtcac
     2701 cgaagagggc ggcgtggtgg acccgaccat cctcgagtcc agaccgggaa ccacgttcga
     2761 cgccgccgcc aaggccgcgg ccatgaggtg gcgtttccgc cccgtcgtcc gcaacggcca
     2821 gcccacgcct atccgagccc gcatccgcat cgacttcgag ctgccggagt gaagggcgcg
     2881 gcgttgacga tgctggtgat gctggccctg ctgccggcat cgcgcctcgc cgcccaggat
     2941 gtgccgtccc cggcgtttgc ccagcagctc gcccgcgtca tggaccgggc ggaggaggac
     3001 cccgagtggg ccctggagcg tctggccgag atgatgcgcg ccaaccgcaa ccggcccacg
     3061 gagctgcggt acgccgtccg gcagcgtgcg gccatgctca tgcagaccga acgtctggac
     3121 ctggccgccg aggagatgct ggcggcggtg accggcaaag acccgggcta cgcccccgag
     3181 ctgcgtttcc tgctgggcca ggtctggctc ctgaaagacg acgccagaac ggcgctgggc
     3241 catctcgagc tctgggcgcg ccatgccgat cccctggacc cggcgggtct gttcctggtc
     3301 ggctacgccc atctgcagct ggacgagttc gagcccgcgg cgaaagccct ggagacggcg
     3361 atcgccactt cgatcaagcc gaggccccag tggttcgaga tcctcgccta cgtctacacc
     3421 cggctcgatc gtcccgccga cgccatcggc cttctggaaa cgctcatcgc ctcgcagccc
     3481 cagcagagcc gctggtggcg ccagctgggt tcgatctacc tgctgatcga cgacgtccca
     3541 aggggcacgg ccggcctcac cgtgcttgcc gagctggagc ccctggggct cagcgacagt
     3601 cgcagtctgg cgaggctgtt cgccagcctc gacatgcccg ccgatgcagc ctacgtcctg
     3661 gatgcggcca tgacacgcgc ccggacggcg gccgagctgc cgggagcgcc gtccgaaccc
     3721 gacggctcgg agaagcccga ggttccgcag gagcttcccg gctacgagga cgtgatgctg
     3781 ctggctgagc tgtggatcat ggcccgggag ttcgacgccg ccgtggggac actcaaaacc
     3841 gcttccgcca tgcgggacga cggcgaggca cagacgctgc tcgggcagct atacctgcag
     3901 cgggaggact acccgcaagc cgatagacac ctcgacgcgg cgctcgccgc ctacggcgaa
     3961 gagcctccac cgcgactgat ctatctgaag gcggttgttg ccctgaaccg gggcgacgat
     4021 gaggcggcca gccgggccct gaagcgaata tccggtgacg aggcattcgc ccagcgcgtc
     4081 gccaggctgc agcgctacat cgacagccgg cgtcagcgcg gcgcctgaag cattctcctt
     4141 caccgcaaca tgcaatggcg cccggcggcc tccttattcc gggccgaatg tgctatgaac
     4201 ccacccatcg ggaaccaggg agagtccatg agctcagctc ggaagcgaag ccgcctgctg
     4261 cagctgctgg ccacgtgctg gctgcttgcc ggggcgggca acgcctgggc cgagcgggcg
     4321 ctggcggcag tagccagcaa cttcgtcgtg cccgccgagc tgctggccgc cgagttcgaa
     4381 cgcgccaccg ggcacacggt ttccatagcg gccggcgcta ccggaaagct atatgctcag
     4441 gtcgtgcagg gagcgccctt cgacctgctt ctcgccgccg acgcctggcg acccgagcgc
     4501 ctggagctcg aaggtcgcgc ggtgcccgga tcgcgattca cctatgccgt gggtctgctc
     4561 accctctgga gcccacgccc ggatgccttc ggcgaggatc cggtaggcta tctgcgcgcc
     4621 ggccgtctgc gccggctcgc ccttgccaac ccggatctgg cgccctacgg aaccgccgcg
     4681 cgccaggtgc tggagcacct cggcctgcag gctcagctgg ccgataaaat ggtgttcgcg
     4741 gaaaacatcg gccaggccca cgccatggtc gataccggca acgccgatgc cggttttctt
     4801 gcccgcgccc aggtgatcgg ccgcccgggc agccactgga acgtgcccgt gggcatgcac
     4861 ctgccaatcg agcagcaggc cgtgcttctc cggcgggcgg cggacaatgc ggcggcaacg
     4921 gggttcctcg ggtttctgcg cacggaagca gcccgcggcg cgatggccac ggccgggtac
     4981 ggattgccgt agatgcccga cctcgcgcca ctgtggctga ctctgcatct ggcgacggcg
     5041 accacgctca tcctgctggt catcggcacg cccatcgcct ggtggctggc gcgcacacgc
     5101 tcgccgctgc gagcccccgt ggaagctctc accgcgctcc cactggtgct gccacctacc
     5161 gtgctcggct tttaccttct cgtcatgctc agcccgacct cgccgttcgg aagcttctgg
     5221 gtacaggtaa ccggtgaggc gctgaccttc tcgttcgcag gactcgtggt ggcatcggtc
     5281 atctactcgc tgcccttcat ggtccagccg ctgacgaacg ccttttccgc catcggcccg
     5341 gggccaatgg aggccgccgc cagcctgggc gccggcccgt tcgacgcgtt ccgttgcgtc
     5401 gccgcgcctc tggccaagcg ggggttcgtc acggccgccg ccttgtcttt cgcccacacc
     5461 gtcggggagt tcggcgtggt cctgatggtg ggcggcaaca tcccgggcga gaccaaggtg
     5521 atctccatcg ccatctacga gcacgtggag accctgcggt acgcagaggc ccatctgctc
     5581 tccgccgggt tggtggcctt cagcttcgtc gttctcctga ccgtttacgg tctaaaccgg
     5641 aggtccccgc tcaatgtcgg ttgacgagct ggacatcgag atcacggcat cgctgcccgg
     5701 tttcgacctg cgcctgaagc ggacgctgcc gctacagggg gtcaccgccg tgttcggacc
     5761 gagcggcagc ggcaagagca ccctgctcag agtgctggcc ggcttcatca ggcccgcgtc
     5821 cggcagggtc tcctgcggcg gggatgtctg gttcgacaag gccgccaacg tgcacctccc
     5881 gccacaccgg cgcccggtgg gcatgatgtt tcaggacgcg aggctattcc cgcacctgaa
     5941 cgtggccggg aacctggact tcgccgtgcg acgtcagcgg cggccaggca acggcatcga
     6001 gcagcccgag gtggtggccg cactggatct gggtcccctc ctcacgcgcc aggtcgattc
     6061 gctctccggg ggcgagcgcc agcgggtggc gctggggcgc accctgctcg cctcgccccg
     6121 gctgctgctg ctcgacgagc ctctggcagc gctggaccgc gaacgcaaag ccgacatcct
     6181 cccgtacctc gatcagatcc agcggggctt cggcatcccg acgctctacg tgagccacga
     6241 cgtcgacgag gtggctcagc tgtgcgaccg cgtgctcgtt ctggccggcg gcagcgagcg
     6301 ggcgttcggc gcaacggccg acatcgtcga gcggctcgac ctggaaccgc tgatgggccg
     6361 cttcgaggcc ggcgttctgc tcgagggcac ggtgaccggg cacgaccctc gcctgcacgt
     6421 cacccacgtc gacgtgggcg gcgacgtcct cacccttccg ctgctcagcg gcgtggcgga
     6481 aggccacgcg gtccgcgtgc gcatacgggc ccgggacgtc gccctggcca ccacccagcc
     6541 agagggtctg agcatccgca acgtgctgcc gggtcgcata tcccggctgc tcatcgagga
     6601 agcgtcgggc accgccgagg ctttcgtcga gctcagggga gcccatctcc gatcgcggct
     6661 cacgctggcg gccgtggagg atctggggct tcgggaaggc atgcccgtct atgcgctcat
     6721 caagagcatc agcttcgaga gcagcgcgcc cggcggctga aactcagctc tgcagctctt
     6781 cagccgtcca gctcttcgag ctccaggcct tcggctctcg cgacgttctc catggcttcc
     6841 tcgaagagcg cacgggcgtt cgcagcgatg agtctcagcc gggcatgcag cacccggtct
     6901 gccgggtcgc cctgctcgca gagcttgctg tactcctcga aggcgctgat gtccgtgagc
     6961 agctgggcga tgtgattggg gcactcacag cggatggcgg gcgccatggc agcgagccgg
     7021 gcaatgctgt ccggcgagaa tcgaggtgcg ggaatcgtcg ggtcggcgag ggccgccacc
     7081 actccgggcg cttcgctctg ggcaagggcc cgcagcacct cctggccgtc aacgggcgcc
     7141 ttgaggcaga cgatgccccc atcgagcaac atctgcaggg tggtgcgggc tgcaaaaccg
     7201 tagacgacga tcacgcgctg gtacacgcct cgccggagct cgagaagcct cccgcacgat
     7261 tcttcgctca acgtcgacag ctccacgacc agagcctgag cctcctgatc cgagccttcc
     7321 tcaccgcctg ccaaccattc ggtcgcaggc cgggtgacca ggcggaacgg tgccggtccc
     7381 agcgcctcgc gggaaagcac cgccgagatc aacggaccca cgacggtaac caggccgaac
     7441 tcggggacgc tgctgcgagc cggcgtggcg ctggccagcg cctccagctc atcatccgcc
     7501 agggtgctca atcgcccgat ctggtggccc tgggccagca ggtcccgcag gaggatgagc
     7561 cggtcgacct gtgactgcgt gaagacccgc cggccggtgg gagtgcgcag aggctcgatg
     7621 acgccgtagc ggctttccca cttccgcagg gtgtgggtgg agatgcccgt cgccttggtg
     7681 acggccccta tggacattac tgccgcttcc ggatctgaca tgtttgcctc ccgatgctca
     7741 gttgatctgg gccagcctca cgcagcgcgg gtggtggtct cgatgaactc gtctgccagc
     7801 gcaaagatct ccggccgctt gcgcacgacc cgcggcaggt tggcgaccat gggcgcaagg
     7861 cgatggttgg ccttcagggt ctcctcgtga tgggcgacga accgccagag cagaccgtcc
     7921 atcacgtcgc accactcgcc gcgcttgaag tcgcccatct tcatgacgta gttcgagccg
     7981 cagatgtatg gcttcgtggt gaagatgcct ccctcgctgg tcagacccat gccgaacacg
     8041 ttggggacca tcacccagtc gtaggcgtcg acgaacattt ccatgaacca gcggtacacc
     8101 tcctggggct cgatgccgga gaggttcatc aggttggccg ccaccatcag ccgctcgatg
     8161 tggtgtgccc agccgagccg aagcgctttt ccgatcacgt gatcgagcgg ggcgattccc
     8221 gtggtgcctc gataccacgc atcggtgagc cgccggtcgg cgttccagat gttgcggctt
     8281 tgcatcggct cgtagtagtg atggaagacg ccgcgcacga actcccgcca gccgatcagc
     8341 tgccgcacga aaccttccaa gctgttgatg ggcacgttgt gctggcccgc gtaatccagg
     8401 gcggcttcga gcacctgctc gggggtcagc agccctacgt tcagaagggg cgccagcccg
     8461 ctgtggtaga cgaacgggga tcgcctggtc agggcgtcct cgtacgcacc gaagttccag
     8521 aagcgggact cgagaaactc atccagccag gtctgggcgc ccgcgtgagt gacgggcaac
     8581 cagaacccgt ccaggtcgcc ggggttgccg gcgaagcgat cggccacgag gtcggacacc
     8641 gagcgaacca gcggcgcgcg cgaaagcaac gccggttcct gggggttgag atcgcgcggc
     8701 agcttttcac ggttgaactg atcgaagttc cagcgcccgc cgaccggcag cccgtcgccg
     8761 ttcaccagga ttctgtgctg gagcctctgc tctttgtaga acggcagcag cttcggcgtt
     8821 ttgtgcttgt cgagatgggc gcggaagtca tccagggtgt tgatgaacat gggcgtccga
     8881 atgacccgca gcttcaagtt cagctgctcg gcaaaacgtg cgatggcgac ggtcagatcc
     8941 gggctttcga gctcgaagtg gcatagcgtg tccgcgctgg tcgccgcgac ggcctctcgg
     9001 accgccgacc gccaggtcgc tgcgctgtcc agatcgaaat atcggacacg ccagccctgc
     9061 ccgcgcagcg tctgcgcgta gctgcgcatg gcggaaagaa cgaaggtgag cttctgcttg
     9121 tggaaagcgt atcgccgaca gaactgctcg tcctccacca tgacgaactc cgcatctgca
     9181 tgctcacgca gatatcgggg gtgaaagagc tgattgccga agctgagaaa gaccttactc
     9241 accgacttgc ctccgttgct gatggatcac cagggcagct cacgcccgtc ccagttgaag
     9301 aacctgcccg agtcctcggg cgatagatcg tgcacgacgg acagaatgtg ccgggcgctt
     9361 tcgtcgggcg tacgcggatc gcgcccgcga acgaacggct cggacagcgg ggtcagggtg
     9421 gttccggggt gcacggccac cacgctggga ccgttgcgca gccgaccgaa ctcgatcgcc
     9481 acgttgcgaa cccccatgtt gagggctgct ttgctcatgc ggtagctgta ccagccgccg
     9541 agagaattat caccaatgct ccccaccatg gcggagagca cggcagcgac ggggcgctga
     9601 tcgtggcgta gcaggtctcg aaaagcccgg atggcggcca gcggggcgaa cgcgttgacg
     9661 cggacggcga gctggagatt ttcaagctcc agctcagcga gcgacttttc gggtcgatga
     9721 gggctcccgg ccccgccatg cagaacgccg ctgcagcaaa ggagcaggtg cacccgatca
     9781 gtgagctgcg ccacccgaga cgcggcgctc tccagatccg ccgggctggt cgtgtccgtt
     9841 tcaaaccaat cgagaccatc gtccgacgat ggcctggtgc gggacagcgc gaagacgctt
     9901 gcgtagtcgg gatgggcaac gagcgcccgg ctgagcgcct cgcccagccc ccctgcggcg
     9961 ccggccacga cggcgacttt tctcgacaaa tcctttccca ttggctcttt ctacaggaaa
    10021 aaacctatac aaacaacaga aaaactggtg tttggttccc tatatctcga atttgtctat
    10081 gtattgtcga atttatcgaa gcggcccttc gcccggcgga aaccgcaggc gcccggccgg
    10141 ccggcttctc tcccccctcc ggtctggttc aggcagccga cttcgcggcc gccgtcagag
    10201 tcgcggacag ctcggcaacc tccggcggaa tcacgtactt gtgcgggctg taccagggca
    10261 tgtaggtctt taagaacatg ggcgcgaccg cagcaaccgc cgccatccgc gtaaggcctg
    10321 aaacccggta ggtctcaccg aagcgggcca cgtcagccgg cgacatgatc ttcatgcagt
    10381 ccaggatgaa ccgccagatg tgggtctgcg cgatccagca catcccggca cggaaaagct
    10441 gatcgccgta gaggtgctcg tagatgtcgt aggcgacgtt tctgtgctct atctcctcga
    10501 gcaggtgcca cttgaagagc tcgccaaagc gacggtctgt ttttggatga tccggaacgc
    10561 gcgccgcgaa cgccttgagc gcggtctggg tggtgtacgc ttcgaagcct tccacgaagc
    10621 ccacgcacca cttgtcgtcc ttggtggcct ggaaccgctc gaaatcggcc ttcagctgcg
    10681 caaagcgctc cgccagcccc gggtagccga actgcaaaat ggcctcgttg aaccgttcgt
    10741 gctgcatgta gtgctgggct tcctggcggc agaagcggtc cacgttctcc ttgagctcag
    10801 ggtcgctgat ctggtccaga acccggcgca gggacttgac cagaaacggt tcgagatagg
    10861 cgacgtacag gctcacgccc gtgctgacgt aggaaaggac gatgtcccct gccgcccact
    10921 tgggctcgaa gtcctcggga ccgggcacgt cgaactccat ctttcgtacg gtgatgggat
    10981 gcatgaggct gtatcacctg ctccggagag aacgggaatt atacacgcgg gaacgaacgg
    11041 tcatgggctc atcctcccat gacgttgcgc accagaccgg atttcagcat cggatggccg
    11101 gcgggctgat aggtcatcac gagggctcga cgtgggcgat cggagcggtt gggcagcgag
    11161 ccgtgcaccg agtgggggct gaaaaagacc agcgagccag cggccaccac catgggcacc
    11221 tggtccctgg cgtcaaaggc gtccggatcc gtgtagaacc cgccgagctg ggtgccgtcc
    11281 gcgcgacccg gcagacatcc tcgtttgtga cttccccgca ccacccggaa acaaccgttg
    11341 gcctcgctgg cgtcgtcgag ggtcaccatc acgttgggca gatggtcgac gtgtcggcac
    11401 tcgtgtatcc agtagggcga atcctgatgc cagccgaagc cggagccttc ccgcgggcgc
    11461 ttgaggttga gcttgtccgt ccacagggcg atctcctccg agccaaccag gtcccgcatc
    11521 ggctcgcaga tacgcgcgtc gtcgaccagg gcgttcaggt ccgcgtcgag ctccgcaacg
    11581 ggctccacga cgcgaatcgt ctcgctgccg gcggcgtgct cgaactgaac ggtgacgtgt
    11641 ccggcatcga cgaaacggtt gccgtccagg acgtaggtcc ttccgctgcg gctcaggcgc
    11701 caggcggcgt ccgccgcccg ctccgccgca agccgcagtg acgagagctc ctgctccgtg
    11761 aatgcgcact cgcggatcag gaaaccgtca cgcgcgtagc gctcagcctc atcggcactg
    11821 agccgcaagc ccctctgact ggacgccggc gtcaacggcc gccggtttcc gggctgctca
    11881 gaacgtcggc gagcgcaggc gcaaaacgct cgcccatgcg aacgtatcca gcagcgttcg
    11941 ggtgcagatc gtcgggcaga tcctcagcat cggccgcacc gaagaggtcg aggccgtcaa
    12001 cgtaggcgag cgcccggtca ccgccggcaa cacgctgctc gaccacctcc tcgatgatct
    12061 cccgcacccg gcgcagcgtc aggcaaccct cacgaatctc gtcgtgaccg ctgaccgtga
    12121 agaaccggcc atcccggccg gggaacgtgg gtccgggccg ggattcggcg ctggggcagt
    12181 agatggggga gatcaggatg atgggcgtgt ccggttttcc ttccctgacg gtgtccagaa
    12241 acccgtgcag cgccggcgtg aacacccgct cgcgcatgga gtcccgattg atcacgttga
    12301 ttccggtctt gatgctgatc acgtcagcgt ccacttcacg aatggtccgg gcgacgaact
    12361 gatccagatg gcactgcccg ccgaaaccca ggttcgtcag gctggcgccc gcccgccgcg
    12421 cggccacggc gggccagatc agtccaggat gatctgcctc catgcaatga ctgatggagc
    12481 tgccgtagtg aacccagcgg ggacgcggat cgagggcggg ctctgcgagg aacgccccgt
    12541 cgtcgagcgt cagctcacgc agctccacat aggcgttgtg aggcagccac agctcgcatt
    12601 gcttttcacc cgcgggcaga tcttcgaaga ggaccgtgtc ggggtcgccc ttcacgagct
    12661 cgaacgcgcc gggccgggcg cggtcgagca gaatcgtgtt gcccctgtcc gatgcgacgc
    12721 tccacaacgc gccgtcgatc tcgaggtcga agggaatcgg gcggcgcgtc tccggcggca
    12781 tcaccatgtt ggtggccagg aaagtcaggc caatggcggc ggcgtcggtg gtgaacgcaa
    12841 gacgcacgcc cgacggcatg cgcaccatca cgtccatctc ccgcggcagt tgcggccgcg
    12901 tccaggccgg cagtcgtcgc gggctgagac cggccggcct gtggtcgaag tccagcgcgc
    12961 cccggacgca tacccggtcc ggcacgatct cggcgatggt gaacgacccc aagcgagcgc
    13021 taccagttct cgaagtgaac gacgccctcc atgggcttgc gcggcgcccg cttgaactcc
    13081 gt
//
LOCUS       DBNHKV010000374         2607 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1823357, whole genome shotgun sequence.
ACCESSION   DBNHKV010000374 DBNHKV010000000
VERSION     DBNHKV010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1823357"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="AC16PP_11995"
     CDS             <1..147
                     /locus_tag="AC16PP_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012314925.1"
                     /note="YghU; B2989; one of eight putative glutathione
                     transferase proteins from E. coli; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HIR6482396.1"
                     /translation="TSYEHVIRWAKEIEERPAVQRGRRVNKVWGSEETQMPERHSASD
                     FGSV"
     gene            176..1522
                     /gene="gdhA"
                     /locus_tag="AC16PP_12000"
     CDS             176..1522
                     /gene="gdhA"
                     /locus_tag="AC16PP_12000"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014755289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HIR6482397.1"
                     /translation="MTITAPDLDQFMRGLRRRNPGESEFHQAVQEVAIDILPYIADKP
                     AYTAKRIFERMTEPDRIVSFRVVWEDDAGNVRVNRGYRVQCNNAIGPYKGGIRFDPSV
                     NQSILKFLAFEQTFKNSLTGLPLGGAKGGADFDVKGKSDSEVMRFCQAFMTELYRHIG
                     PFTDVPAGDIGVGSREVGYLFGQYKRLANRFEGVLTGKALEFGGSHIRTEATGYGVVY
                     MMENMLAHRGESLEGRTCAVSGSGNVAIYTVEKLNELGARVVTMSDSSGFIHDPDGID
                     ADKLAWILDLKTVRRGRVSEYADEFGVEFQEGKRPWAVPCELAFPCATQNEIGKEDAE
                     TLVANGCCAVVEGANMPTVPEALEVFTSARTLLAPSKAANAGGVGVSGLEMSQNSVRL
                     GWTREEVDTRLRQIIKEIHGHCVEHGDEGDYVNYLKGANIAGFRKVAAAMLAYGVM"
     gene            complement(1531..>2607)
                     /locus_tag="AC16PP_12005"
     CDS             complement(1531..>2607)
                     /locus_tag="AC16PP_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6482398.1"
                     /translation="INDYEHAEIEPDGAVATEFANEAWELRAELVYELEAWRGALGLQ
                     HSDRDFSAIGEEAYVPPVQTTDSGLFWVAERDFGGFDLEAGARIGRLEHEPLGAPSER
                     FTTYGLSLGAVVPLGAGWRLGLLGDLSSRAPVAEELYSDGPHLATRAFEIGDPALDSE
                     KAFNLSATLEFEGERWSLAGTVYYTGFSDFIYQQATGGVEDGLPVYVYRQEDARFVGV
                     DASVEWRAAAWEGGDLRLRGLFDLVDAKLDVSGNDNLPRIPPMRYGVGAQMSFGPVTA
                     SLDYLRVEDQDDAAPGELPTQGYDDLQAYLGVELPFAADHRLTMFVSGRNLTDDEQRL
                     HTSFIKDFAPAPGRTIEAGLRYLF"
BASE COUNT          509 a          830 c          847 g          421 t
ORIGIN      
        1 acgtcctacg agcacgtgat tcgctgggcc aaggagatcg aggagcgccc cgccgttcag
       61 cgcggccgcc gggtcaacaa ggtctggggg tcggaggaga cccagatgcc agagcgtcac
      121 agcgccagcg atttcgggtc ggtttgatgg gcttgtcgag gtcagaagaa gcgccatgac
      181 aatcaccgct cccgatctgg accagttcat gaggggcctg cgccgccgca acccgggcga
      241 gtccgagttt caccaggcag tgcaggaagt ggccatagac atcctgccgt atattgctga
      301 caagcccgcc tacacagcaa agcggatctt cgagcgcatg acggaaccgg atcgcatcgt
      361 ctccttcaga gtcgtctggg aggacgacgc cgggaacgtg agggtgaacc gcggctatcg
      421 ggttcagtgc aacaacgcca ttggacctta caagggcggc atccgcttcg acccgtcggt
      481 caaccagagc atcctcaagt ttctcgcctt cgagcaaacc ttcaagaaca gcctcaccgg
      541 tctgcccctg gggggtgcca agggcggcgc ggacttcgac gtaaagggaa agtccgactc
      601 ggaagtcatg cggttctgtc aggcgttcat gacggagctg taccgacaca tcgggccttt
      661 taccgacgtg cctgccggag acatcggggt cgggtcccgg gaggtcggct acctgttcgg
      721 tcagtacaag cggctggcca accgcttcga gggcgtgctg accggcaagg cgctcgagtt
      781 cggtgggagc cacatccgca ccgaagccac cggctacggc gtggtctaca tgatggagaa
      841 catgctcgcc catcgagggg agagcctcga gggcagaacg tgtgccgtat ccggctccgg
      901 caacgtggcc atctacaccg tggagaagct aaacgagctc ggcgccaggg tggtcaccat
      961 gtccgactca tcaggattca ttcacgaccc ggacgggatc gatgcggaca agctcgcctg
     1021 gattctggat ctcaaaaccg tccgccgcgg tcgcgtgagc gagtacgcgg acgagttcgg
     1081 cgtcgagttc caagaaggca aacggccctg ggccgtgccc tgcgagctcg catttccctg
     1141 cgcgacccag aacgagatcg gcaaagagga tgccgagacc ctcgttgcca acggctgctg
     1201 cgcggtggtc gagggcgcca acatgcctac cgtgcccgaa gcgctggagg tcttcaccag
     1261 cgcccgaact ctgctcgcgc cctccaaggc ggcgaacgcg ggcggagtcg gcgtgtccgg
     1321 gctggagatg agtcagaaca gcgtgcgtct gggctggacg cgcgaggaag tcgacacgcg
     1381 cctgcgccag atcatcaaag aaattcacgg tcactgcgtg gagcatggcg acgaaggcga
     1441 ctacgtgaac tacctcaagg gcgcgaacat tgccggcttc cggaaggtgg cggcggccat
     1501 gctggcttat ggggtgatgt gagaagcccg tcagaacagg taacgcagcc cggcttcgat
     1561 ggtacggccg ggcgccgggg cgaagtcttt gatgaacgag gtgtgcagac gctgctcgtc
     1621 atcggtcagg tttctgccgg agacgaacat cgtcaggcga tgatcggccg cgaaaggcaa
     1681 ctccacacct aggtatgcct gaaggtcgtc gtacccctgg gttggcagct cccccggcgc
     1741 cgcgtcgtcc tgatcctcga ccctgagata atccagcgag gccgtgacag gcccgaacga
     1801 catctgggcg ccgacgccgt aacgcatcgg gggtatgcgg ggcaggttgt cgttgccgct
     1861 gacgtcgagc ttggcatcga cgaggtcgaa cagcccccga aggcggagat cgcctccttc
     1921 ccaggcagcg gcccgccact caaccgaggc gtccacgccc acgaagcgcg catcctcctg
     1981 ccggtagacg tagaccggca ggccgtcctc cacgcctccc gtcgcctgct gatagatgaa
     2041 gtccgaaaaa ccggtgtagt aaacggttcc ggccaacgac cagcgctcgc cttcgaattc
     2101 cagggtcgcc gacaggttga aagccttctc ggagtccagt gccgggtccc cgatctcgaa
     2161 agcgcgggtt gcaaggtgag gcccatcgga atagagctcc tcggcaacgg gcgcccggga
     2221 agagaggtca cccagaagac cgaggcgcca tcccgccccg aggggcacca cggcaccaag
     2281 ggagagcccg taagtagtga accgctcgct cggcgcgccc agcggctcat gctccagccg
     2341 gccgatgcgc gcgcccgctt ccaggtcgaa acctccaaag tcccgctcag caacccagaa
     2401 caggccgctg tcggtcgtct gcaccggagg cacgtaggcc tcttccccga tggcggagaa
     2461 atcacggtct gagtgctgca gcccgagcgc cccgcgccag gcctcgagct cgtataccag
     2521 ctcggcccga agctcccagg cctcgttggc gaactccgtt gccaccgcgc cgtccggttc
     2581 gatctctgcg tgctcgtagt cgttgat
//
LOCUS       DBNHKV010000375         3349 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1470725, whole genome shotgun sequence.
ACCESSION   DBNHKV010000375 DBNHKV010000000
VERSION     DBNHKV010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1470725"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="AC16PP_12010"
     CDS             <1..415
                     /locus_tag="AC16PP_12010"
                     /inference="COORDINATES: protein motif:HMM:NF037007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482399.1"
                     /translation="LRVRVSGQGGSDPAAQLRALITGFVRFSASFPEANRLMIREGMD
                     DDWRLQWLAEHIVGPWYERLGAAFEEARSAGAVRNVPFVHFYYILTGAGSLLFSMAPE
                     AERLAGIDPYDDDVVRRHAEVIADLLIPGSGEHAH"
     gene            412..1962
                     /locus_tag="AC16PP_12015"
     CDS             412..1962
                     /locus_tag="AC16PP_12015"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: protein motif:HMM:NF004829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HIR6482400.1"
                     /translation="MSSYDVAIVGMGPVGAAAAILFADAGLKVIAFERDAHVYPLPRA
                     VALDGEVVRGFQRVGRAGELAALLQPVRPGERAGFANSRREWLFGQPYATFGVNGWQP
                     ISMFDQPELEAHLRRLAMDHSGVTGCLEVQVETFEETGGQVAIRARQADGALSETSAR
                     FLVACDGAASGARRALEIGWRDLGYDHDWLVVDVVLKDGHTLGLDTLQVCDPDRLSTY
                     IATKDPFRRWEFKLNPGETREEMLEPERIRELIDPWTPRGTYEIRRSAVYQFHAATAE
                     TWRKGGVLLAGDAAHQTPPFLGQGLNAGMRDVINLAWKLPLVLSGAADEGLLDSYQAE
                     RGAHAADLVEWAVAIGKLMEHLAAVEAAQRVGRKPPEVPDGNRASGYGQGREAPPLRD
                     GVVMVDQVSEDGSTGYLFRQPAVRDADGEEYLLDDRLGAGFALVARSEADLAMSEASR
                     AIVDALGMSTTTLEGLEATRGRFDRLFEGSAAAVVRPDRYVFGHTSQSLSLDALLGEL
                     AARLGLRL"
     gene            complement(1997..3205)
                     /locus_tag="AC16PP_12020"
     CDS             complement(1997..3205)
                     /locus_tag="AC16PP_12020"
                     /inference="COORDINATES: protein motif:HMM:NF052847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6482401.1"
                     /translation="MNANAKRILVWSVVAIAVAVGLTIAFMPRPVTVDLITVIPAPME
                     VTADEEGETRVHDVFVLSAPVAGRLRRIDAHVGDPVLADETVLARIEPADPTFLDPRT
                     EAQARAARQAAVSALTLAKARVEEARAEFEYADAEFHRARELIAKGTISVRDAEAAER
                     AYKARRAALSTAQAAVQMRTYELQQAEALLISPAQSRTRTDECDCVDVTAPVSGQVLQ
                     IVNPSERIVTAGEALVEIGDPGDLEIVVDFLSADAVQIEPGQRVIIERWGGESALVGH
                     VRRIEPFGFRKTSALGIEEQRVNVIVDFTSPRNEWSRLGHGYQVDVRVVLWEQDAVLA
                     IPLTALFRDDQRWAVFVDDDGRAALREVVVGHRNGMVAEIVSGLARGDRVVLHPSNRV
                     IDGVRITGRG"
BASE COUNT          501 a         1122 c         1170 g          556 t
ORIGIN      
        1 gttgcgggtt cgcgtctccg ggcaaggtgg ctcggacccg gcagcacagc ttcgcgcgct
       61 cattaccggc ttcgtgcgct tctcggcgag cttccccgag gccaaccggc tcatgatccg
      121 cgagggcatg gacgacgact ggcggctaca gtggctcgca gagcacatcg tcgggccctg
      181 gtacgagcgt ttgggcgccg cgttcgagga agcccgatcc gcaggggccg tgcgcaacgt
      241 ccccttcgtt cacttctatt acatactcac gggggcggga tcccttctct tctccatggc
      301 tcccgaggcc gagcgccttg caggcatcga tccgtacgac gacgatgtgg tgcgcagaca
      361 cgccgaagtc attgcggatc tgctcattcc tggtagcggg gagcacgcac attgagcagc
      421 tacgacgtcg ccattgtggg catggggccg gtgggcgctg ccgccgccat cctcttcgcg
      481 gacgcgggcc tgaaagtcat cgccttcgaa cgcgacgcgc acgtctaccc gctgccgcgg
      541 gccgtcgccc tggacggcga ggtggttcgc ggctttcagc gggtggggcg ggccggcgag
      601 ctcgccgctc tgctgcagcc ggttcggccc ggcgagcggg ccgggttcgc gaactcccga
      661 cgcgagtggc tctttggcca gccttacgcc acgttcgggg tcaacggctg gcagcccatc
      721 tccatgttcg atcagccgga gctggaggcg catctccgcc ggttggcgat ggaccactcc
      781 ggggtgactg ggtgccttga ggtccaggtc gagaccttcg aagaaaccgg tgggcaagtc
      841 gccattcgcg cgcggcaggc ggacggcgcg ctttccgaga cgagcgcccg gttcctggtc
      901 gcctgcgacg gcgcggcgag cggcgcgcgc cgggccctgg agatcggctg gcgcgacctg
      961 ggctacgatc acgactggct ggtggtggac gtcgtgctga aagacgggca caccctgggt
     1021 ttggatacgc tgcaggtctg cgaccccgac aggctgtcca cctacatcgc gaccaaggac
     1081 cctttccggc gctgggagtt caagctcaat cccggcgaga cccgggagga gatgctagag
     1141 cccgagagga tccgcgagct gatcgatccg tggacaccac ggggcaccta cgaaatacgg
     1201 cgcagcgccg tgtatcagtt tcacgccgcc accgctgaga cctggcgaaa gggtggcgtg
     1261 ctgctcgcgg gggacgccgc ccaccagacc ccgccctttc tgggtcaggg gctgaacgcc
     1321 ggaatgcgcg acgtgatcaa tctggcctgg aagctcccgt tggtcttgag cggtgcggca
     1381 gacgaaggtc tcctggattc ctatcaggcg gaacgggggg ctcatgccgc cgacctggtg
     1441 gaatgggcgg tggccatcgg caagctcatg gagcatctgg ccgccgtgga agcggcacaa
     1501 agggtggggc ggaagccgcc tgaagtgccg gacggcaacc gcgcgtccgg ctacggccag
     1561 ggtcgggaag caccgcccct gcgcgacggc gtggtgatgg tggatcaggt gagcgaggat
     1621 ggctccacgg gctatctttt ccgccagcct gcggtgcggg atgccgatgg ggaggagtat
     1681 ctcctggatg atcggctggg tgccggcttc gcgttggttg ccaggtccga ggccgatctg
     1741 gccatgagcg aggcatcccg ggccatcgtg gacgccctcg gcatgtcgac cacgaccctg
     1801 gaggggctcg aagccacccg gggacgcttc gacaggctct tcgaaggctc ggccgctgcc
     1861 gtggtgcgac ccgaccgcta cgtgttcgga cacacctcgc aatccttgag cctggatgct
     1921 ctcctcgggg agctggcagc aaggctgggc ctgcggctct agcctctact gcggcgccgt
     1981 aggccccccg gcctcgtcag cctctgccgg tgatccggac gccgtcgatc acgcggttgc
     2041 tcggatgcag aaccacccgg tctccccggg caagaccgct gacgatctcg gccaccatcc
     2101 cgttgcggtg gccgacgacg acctcgcgca gggcggcgcg gccgtcgtcg tcgacgaaca
     2161 cggcccaccg ctgatcgtcg cgaaacagcg ccgtcagcgg tatcgcgagc accgcgtctt
     2221 gctcccacag caccacgcgc acgtcgacct gatagccgtg acccaggcgc gaccactcgt
     2281 tgcgcgggct cgtgaaatcc acgatcacgt tcacccgctg ctcctcgatc cccagggccg
     2341 acgtcttgcg gaacccgaag ggctcgatgc gccgcacgtg gccgaccagg gcggactcgc
     2401 cgccccagcg ctcgatgatg acccgctgcc cgggctcgat ctgaaccgcg tcggcggaca
     2461 gaaaatccac cacgatctcc aggtcgcccg ggtcgccgat ctccacgagc gcttcgcctg
     2521 cggtcacgat ccgctcgctc gggttgacga tctgcagcac ctggccgctc accggcgcgg
     2581 tcacgtccac gcagtcgcac tcatcggttc tggttctcga ttgcgcgggc gagatcagca
     2641 gcgcctctgc ctgctgaagc tcgtaggtgc gcatctgcac cgcggcctgg gccgtgctca
     2701 gggcggcccg cctcgccttg taggcgcgct cggccgcctc ggcgtcgcgg acggagatgg
     2761 tgcccttggc gatcagttcc cgggcccggt ggaactcggc gtcggcgtac tcgaactctg
     2821 cccgcgcttc ctcgactcgc gcctttgcca gggtgagcgc agagactgcc gcctgccgcg
     2881 cggcgcgggc ctgggcctcc gtgcgcgggt cgagaaaggt cggatcggcc ggctcgatgc
     2941 gggccaggac ggtttcgtcg gcaaggaccg ggtcgccgac atgggcatcg atgcggcgca
     3001 gccggccggc gacgggagcg gacagcacga agacgtcgtg aacgcgcgtc tcaccttcct
     3061 cgtcggcggt gacctccatg ggcgccggaa tgaccgtgat caggtccacg gtcaccggtc
     3121 tgggcatgaa tgcgatcgtc aggccgacgg ccacggcgat ggcgacgacg gaccagacca
     3181 gtatccgctt ggcgttggcg ttcatttcta ctcccgggtc ttgagcactt ctatcatgtt
     3241 caggcggtcg acgcgtcggc gaacgagggc cgcggacagc gtcgtggccg ccagcgcgat
     3301 gagcacggct atgccgtacg tggacggatc gatggtcagg ggcacgcgg
//
LOCUS       DBNHKV010000376         3745 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1895029, whole genome shotgun sequence.
ACCESSION   DBNHKV010000376 DBNHKV010000000
VERSION     DBNHKV010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1895029"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3061)
                     /gene="gltB"
                     /locus_tag="AC16PP_12025"
     CDS             complement(<1..3061)
                     /gene="gltB"
                     /locus_tag="AC16PP_12025"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HIR6482402.1"
                     /translation="MSIRTPWGFPPKRGLYDPANEKDSCGVGFICDIKGRASRDIIDQ
                     AQHMNCCMTHRGGVGYERNTGDGAGILTGLPDKFLRRVAASELGVTLPPQGQYGVGNV
                     FLPNDEAERRHCIAIFEREIQAAGQKLLGWRELPVHPDGAGIGHAARAAMPTFAQLFV
                     GAKDASGDAFERKLYLIRKHSTHLLRGDMALGERKLFYVCSLSSKVIIYKGMLTPEQV
                     FPFYQDLDDPDYESHLAMVHSRFSTNTFPSWDRAQPNRFMSHNGEINTLLGNVNSMNA
                     RQGLLSSPLFGADIEKLYPIVEEDCSDSGTFDNVLEFLLMSGRKLQEAVLMMIPEAWQ
                     QHPTMDQAKRAFYEYHSCLMEPWDGPASIAFTDGTYIGAVLDRNGLRPSRYYITNDDR
                     CIMASEVGVVPVDPACVLEKGRLQPGRIFLIDFEQGRMIPDAEVKQEWVKARPWQQWL
                     DNQRIDLAEIPPGDDPHGFHPANLTPRMQAFGYTVETMEIMLQPLVKELRDPLGSMGN
                     DAALAVMSDKPRMIYDYFKQRFAQVTNPAIDSIREEVIMALECYIGPERNLLETTEEH
                     AHRLRVPHPILSNEELAALKHMDHRGWRSRTIDITFKQGTGRDGLVAALDRICAEASG
                     AIADGYSLVVLSDRGIGPDRVPVSSLLAVGAVHHHLVREQSRTRIGIVLETGEAREVH
                     HHCLLVGFGADAINPYLAFEALWQARRNGYLDDAAHITSDDDVVAAYRKGTAKGMLKV
                     MAKMGISTLHSYKGAQIFEAVGLADDVIDVAFVGTASRVQGIGLRVLAEEMERRHVIG
                     YSKRSQNVTVLPNPGDFHWRRTGDTHMWDPSAIASLQVAVRGNSEDAYWQFARHVNEE
                     TTRHANLRGLLTFNKDVNGGPIPIEEVEPATEIVKRFATGAMSFGSISAEAHESLAIA
                     MNRLGGKSNTGEGGEDPSRFIPLENGDSKRSAIKQVASGRFGVTINYLTNADELQIKI
                     IQGAKPGEGGELPGHKVDDIIANIRHSTPGVGLISPPPHHDIYSIEDM"
     gene            complement(3345..>3745)
                     /locus_tag="AC16PP_12030"
     CDS             complement(3345..>3745)
                     /locus_tag="AC16PP_12030"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF049302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6482403.1"
                     /translation="EDFALVPAEHWLDIYAELTGMPDTARKLHGAIVAGIPTPCGFAV
                     LRRGERPVACGLAVVERDLLGLFDIVTDPDARRSGHGTALVGSLLGWGHARGARLAYL
                     QMIESNVPASALYRKLGFEIAYNYWYRISP"
BASE COUNT          608 a         1143 c         1281 g          713 t
ORIGIN      
        1 ccatgtcctc gatggagtag atgtcgtgat gaggcggcgg gctgatgagc ccgacgccgg
       61 gcgtcgagtg gcggatgttg gcgatgatgt cgtccacctt gtggccgggc agctcgccgc
      121 cctcgcctgg cttggcgccc tggatgatct tgatctgcag ctcgtcggcg ttggtgagat
      181 agttgatcgt cacgccgaac cgaccgctgg ccacctgctt gatggcgctc cgcttggaat
      241 ccccgttctc cagcggaatg aagcggctgg ggtcctcgcc gccctcaccg gtgttcgatt
      301 tgccccccag gcggttcatg gcgatggcca gcgactcgtg ggcctcggcg ctgatcgagc
      361 cgaagctcat ggcgccggtg gcgaaccgct tgacgatctc cgtggccggc tcgacctcct
      421 cgatggggat agggccaccg ttgacgtcct tgttgaacgt gagcaggccg cgcaggttgg
      481 cgtgacgggt ggtttcctcg ttgacgtgcc tggcgaactg ccagtacgcg tcctcgctgt
      541 tgcctcgcac ggcaacctgc aggctggcga tggcgctggg atcccacatg tgcgtatcgc
      601 ccgtgcgccg ccagtggaag tcgcccgggt tgggcagcac ggtgacgttc tgggaccgct
      661 tggaatagcc gataacgtgg cggcgctcca tctcttcggc cagcacccgc aggccaatgc
      721 cctggacacg gcttgcggtg ccgacaaagg ccacgtcgat gacgtcgtcg gcgaggccga
      781 cggcctcgaa tatctgcgcg cccttgtatg agtgcagggt cgagatcccc atcttcgcca
      841 tcaccttgag catgcctttc gccgtgccct tgcggtaggc ggcgaccaca tcgtcgtctg
      901 aggtgatgtg ggcggcgtcg tcgagatagc cgttgcggcg ggcttgccag agcgcctcga
      961 aggccagata cgggttgatg gcgtcggcgc cgaaacccac cagcaggcag tggtggtgca
     1021 cctcccgggc ttctccggtt tccagcacga tgccgatacg ggtcctggac tgctcacgga
     1081 ccaggtgatg gtgcacggcg ccgacggcga gcagcgagct caccgggacc cgatccggcc
     1141 cgataccgcg gtccgagaga acgacgaggc tgtagccgtc ggcgatggcg ccactggctt
     1201 ccgcgcagat ccggtcgagc gcggccacga gcccgtctct gccggtgcct tgcttgaagg
     1261 tgatgtcgat ggtgcgcgaa cgccagccgc ggtggtccat gtgcttgagc gcggccagct
     1321 cctcgttgga aagtatcggg tgcggcaccc ggagccggtg ggcgtgctcc tcggtggttt
     1381 cgagcagatt gcgttccggc ccgatgtagc actccagcgc catgatcacc tcctcgcgga
     1441 tggagtcgat ggccgggttg gtcacctggg cgaagcgctg cttgaagtag tcgtagatca
     1501 ttcgcggctt gtcggacatg accgcaagcg cggcgtcgtt gcccatggac cccaggggat
     1561 ctcgcagctc cttgaccagc ggctggagca tgatctccat ggtctcgacg gtgtagccga
     1621 acgcctgcat gcgcggcgtc aggttcgcgg gatggaagcc gtgcggatcg tcgccgggtg
     1681 gtatctcggc cagatcgatt cgctggttgt ccagccactg ctgccagggg cgggccttca
     1741 cccactcctg tttgacctcg gcgtccggaa tcatgcgacc ctgctcgaag tcgatgagga
     1801 agatccgacc gggctgcagg cgccccttct cgagcacgca ggcggggtcg acgggcacga
     1861 cgccgacctc ggaggccatg atgcacctgt cgtcgttggt gatgtagtag cggctcgggc
     1921 gcaggccgtt gcggtcgagc acggcgccga tgtatgtccc gtcggtgaag gcgatggatg
     1981 cggggccgtc ccacggctcc atcaggcagg agtgatactc gtaaaaggcc cgtttcgcct
     2041 gatccatcgt cgggtgctgc tgccaggcct ccggaatcat catgagcacc gcttcctgca
     2101 gcttgcggcc gctcatcagc aggaactcga ggacgttgtc gaaggtgccc gagtccgagc
     2161 agtcctcctc gacgatcggg tagagcttct cgatgtccgc gccgaacagc gggctgctga
     2221 gcagaccctg gcgggcgttc atggagttga cgttgccgag cagggtattg atctcgccgt
     2281 tgtggctcat gaaccggttg ggctgtgccc ggtcccacga cgggaaggta ttggtgctga
     2341 aacgcgagtg gaccatggcc agatgcgatt cgtaatcggg atcgtccagg tcctgataga
     2401 agggaaagac ctgttcgggc gtcagcatgc ccttgtagat gatcaccttg gaggagaggc
     2461 tgcagacgta gaagagtttt cgctcaccga gcgccatgtc cccacgcaga agatgggtgc
     2521 tgtgcttgcg gatcaggtag agcttgcgct cgaacgcatc gccggatgcg tccttggcgc
     2581 ccacgaacag ctgggcgaat gtgggcatgg cggcccgggc cgcgtggccg attcccgcgc
     2641 cgtcggggtg cacgggcagt tcccgccagc cgagcagctt ctgacccgcc gcctgaattt
     2701 cccgctcgaa gatggcgatg cagtgtcggc gctcggcttc gtcgttggga aggaaaacgt
     2761 tccccacgcc gtactggccc tggggaggaa gggtgacgcc cagctcgctg gcggcgaccc
     2821 gccgcagaaa cttgtcgggc aggcccgtca ggatcccggc cccgtcgccc gtgttgcgct
     2881 cgtagccgac gccgccgcga tgggtcatgc agcagttcat gtgctgggcc tgatcgatga
     2941 tgtcccggct cgcgcgaccc ttgatgtcgc agatgaagcc gacgccgcag ctgtctttct
     3001 cgttagccgg gtcgtacaga cctcgcttcg ggggaaaccc ccatggcgtt ctaatgctca
     3061 tgcaactgtt ctctttcgtg tttggcccgg ccgcttcgac ctcaatggtg ggagccagac
     3121 gccgacgccg cccaaagtgg gagcggatgt cgggcgcgac cgcagccgtc ggcttccgcc
     3181 acggaagcgc gcaggtcctg tcggagatct gcttgccgtt tataacttac tgattcttat
     3241 atatttgctg gcaagctagc tgcggtgtgt ccgcgcgaaa gacaaaacat ctcaaaaatg
     3301 cgggtatgaa tcaagttcgg ccgcctagga acaaaaggtt atggctaggg cgaaatccgg
     3361 taccaatagt tatatgcgat ctcgaagcca agctttcggt agagcgcgct ggccggcacg
     3421 ttggattcga tcatctgaag atatgccagc cgggcaccgc gggcgtggcc ccagccgagg
     3481 agggaaccca ccagggcggt cccgtgaccg gaacggcggg cgtcagggtc cgtgacgatg
     3541 tcgaacaggc cgagcaggtc acgctcgacg accgccaggc cacaggcgac cgggcgctcg
     3601 ccgcgtcgca ggacggcgaa cccgcagggg gttggaatgc cggcgacgat cgcgccgtgc
     3661 agctttcgcg cggtgtcggg catgcccgtg agttcggcgt agatgtcgag ccaatgctcc
     3721 gcgggaacga gcgcgaaatc ctcgt
//
LOCUS       DBNHKV010000377         9746 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_975630, whole genome shotgun sequence.
ACCESSION   DBNHKV010000377 DBNHKV010000000
VERSION     DBNHKV010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9746
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_975630"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="AC16PP_12035"
     CDS             complement(<1..442)
                     /locus_tag="AC16PP_12035"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6482404.1"
                     /translation="MRVEVGGHCAFAGTGSVPFDARRPCVVFLHGAGFDHSVWVMPSR
                     YFARHGFAVVAPDFPAHGRSKGAPLASVPALASWLAELLDALGVAEAVVVGHSLGSLV
                     GQAFARLFPDRCLGLALLGTSAPMAVTEQLLGASRDDDHAAIDMA"
     gene            complement(439..1764)
                     /locus_tag="AC16PP_12040"
     CDS             complement(439..1764)
                     /locus_tag="AC16PP_12040"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HIR6482405.1"
                     /translation="MAGTKHQAPRPTGFDTLALHAGARPDPATGARATPIYQSAAFVF
                     PDSDFAAGLFNIERAGHVYSRLSNPTNAVLEERIAALDGGVGAIATASGQAAMHLAVA
                     TLLGAGDHIVASRSLYGGTHNLLAYTLPRFGISTTFVDPRDPQAFADAVREETRLVFG
                     EILGNPGLEVMNVPAVADAAHAAGLPLMIDATFTTPYLFRPLDHGADLVVHSATKFLS
                     GHGVVIGGLIVDGGTFDWEASARFSTLSQPYDGFHGLVFAEEFGPEAFIMRARKEGLR
                     DFGACMAPMTAFQILQGLETLPLRMDRHVANARAIADFLAGHPMVTAVNHPDLAGHPD
                     HDLAKTLLPRGAGAVMGFDIKGGREAGRAFIESLQLFSHLANVGDAKSLVIHPASTTH
                     HRMDAKALEAAGIGEGLIRVSVGLEDPADLIDDLRGALKRAERAAGVST"
     gene            complement(1846..2610)
                     /locus_tag="AC16PP_12045"
     CDS             complement(1846..2610)
                     /locus_tag="AC16PP_12045"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HIR6482406.1"
                     /translation="MDRVVDLTGAVNFRDFGGYAAADGATVRRGLLFRCGQLASLSQE
                     ALAQFAELGIDVICDLRRPDEREVDPTPVPHHVSRRVEIPMDPGSATQLRDSLRAGYV
                     DVSQRVDFMRTITRELARDHVEGYSSMFAALLEHAPGGFLVHCTAGKDRTGVGVALIL
                     LALGVPRETVIHDYMLTNDVMDFEAFILPRIRQNLGHDNIDVEGARVLSGVREEYIMA
                     ALEEMESACGSLEAYLERAIGLTRGDVDALRAHYLS"
     gene            complement(2686..3081)
                     /locus_tag="AC16PP_12050"
     CDS             complement(2686..3081)
                     /locus_tag="AC16PP_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HIR6482407.1"
                     /translation="MNNTHSLAVGYILWIFGFLGAHRFYFGRPVSGTIYFFTLGLFLI
                     GWLIDLILMPNLEASADRRYQQGEKSYTVAWVLLTFLGFFGVHRFYQGKWMTGIIYLF
                     TVGLFGIGVIYDYWTLNEQVSALNHERAG"
     gene            3109..3426
                     /locus_tag="AC16PP_12055"
     CDS             3109..3426
                     /locus_tag="AC16PP_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482408.1"
                     /translation="MLHVAPNRRRPSTVTCRELRNAPDDLGPDRLRQIVPDALEHQES
                     GPLERLVGIHAALHGDEGVVAAVNEESRDVELLRRRPPVTGRGDGDAGDLARKTFGVE
                     AAV"
     gene            3548..4504
                     /locus_tag="AC16PP_12060"
     CDS             3548..4504
                     /locus_tag="AC16PP_12060"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6482409.1"
                     /translation="MQQLPDPVRIPTNDIELEVFEQGAGAPVVLCHGWPEHAYSWRHQ
                     MSPLAEAGYHVIVPNQRGYGHSSRPQKVTDYDITHLAADLTGLLDHFGYDRAVFCGHD
                     WGAIAVWNLAMLHPDRVAGVINLSVPLLRRGPTDWVSHWESLLGEDFYIVHFNRRPGV
                     ADAVFEENPRRFLGNLYRTHQWQEPAAEPRSGMPLINLARANRPRGRPLMSDAELDVF
                     VRAFEHSGFSGGINWYRNFARNWKLTADVPEHVPHPAMMIYGRYDIVPVFDKLRDFVP
                     LVETHVLDCGHWIQQELPGETNRLMLDWLERNYPSNRRRSSQ"
     gene            4501..4989
                     /locus_tag="AC16PP_12065"
     CDS             4501..4989
                     /locus_tag="AC16PP_12065"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6482410.1"
                     /translation="MNAIKGLNHAAYRCRNSEETRQFYEDFLGLPLVDALPIGTTKTG
                     RAAQVLHTFFAMDDGSCVAFFEEPDAAFDFKDQRDFDLHLALTVDMETLHEMFERGKA
                     QGIETRGISDHGFIHSIYFRDPNGYVVELAAPVELRSGASADGRRLRARQVLDEWEAR
                     RP"
     gene            complement(5048..5446)
                     /locus_tag="AC16PP_12070"
     CDS             complement(5048..5446)
                     /locus_tag="AC16PP_12070"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6482411.1"
                     /translation="MSLESRANRCFVCGPGNPIGLHVRFRLEEDVCLAEFTPGEEHVG
                     YDKVAHGGIIFSLLDDVMANWIWLRGEQCFTARADVRYRAQLPVGTPVRLEGRCLKRK
                     GRLAIMEGKVIRTDTEEVVAEASGSFMIPS"
     gene            5490..6098
                     /locus_tag="AC16PP_12075"
     CDS             5490..6098
                     /locus_tag="AC16PP_12075"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6482412.1"
                     /translation="MHDLNLIRSRLSEHRAEEFAPEPDMREAAVAVILREVSSRPAEI
                     LFIKRAEKPGDPWSGHMAFPGGHRDPEDESLRAAAERETLEEIGLDLDSARYLGPLNQ
                     QRAVARGRPLNMLIAPHVFEIAGDPSFTINYEVAQVVWTPLAPLVSGALHDTETRPLA
                     GTPTVFNGYRLPQGHFVWGLTYRMVKSFFAAIDPGWEPPPEV"
     gene            complement(6115..7497)
                     /locus_tag="AC16PP_12080"
     CDS             complement(6115..7497)
                     /locus_tag="AC16PP_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR6482413.1"
                     /translation="MGVGIDFGTTNSAAATYDGERLQLVALEGADPIMPSATYIDREL
                     QTLTGQAAINQYIADNTGRTVELVPEVIAETSAFVEHGDSENPAPVETVTQKIYGQPL
                     IDSGLHGRLFRGTKRLLGDPRVRRLMVFDHPFRLVALITPILLRMRKALSRTVSGADY
                     AHLGHPVNFEGRDEFRNQTALARLGEAYKYAGITEQRFYPEPIAAAVSYLFAHPEAAG
                     EHLLTLDFGGGTLDFCILRRAAGDFEVVTTHGIALGGDHIDQRLFRELLFPLLGKGET
                     WRRRGMDREIETVFPFEDYQELLVNWAVTYTLNQNRYTTPVLECIGQGGPGARKFRRL
                     RELIQQNLSYVVFQTIKDFKADLSAREEAVLDISEIDVEVRLTRAGFESLIGDLLEQA
                     EQALDETVALAGLVHGDVDIVLCTGGSSLIPAVRRILEDRFPGRVVEHDPFTSVAAGL
                     AIANFHGMDV"
     gene            complement(7573..7956)
                     /locus_tag="AC16PP_12085"
     CDS             complement(7573..7956)
                     /locus_tag="AC16PP_12085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HIR6482414.1"
                     /translation="MGTSLDDARYFNLATFRKNGSAVETPVWFAPSAGEAPGVYYVFS
                     AGDAGKVKRLRRDQTARVAACDVKGKLQGEWLQARAELIEGADAQAALVALRLRYGWQ
                     MRLADLLSRLTGRFRRRAYIRVTLA"
     gene            complement(8001..8654)
                     /gene="msrA"
                     /locus_tag="AC16PP_12090"
     CDS             complement(8001..8654)
                     /gene="msrA"
                     /locus_tag="AC16PP_12090"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR6482415.1"
                     /translation="MAWEQKLRIPSESEALPGRAEEMPVPAAHHVNGAPLKEPFPEGL
                     AQAVFALGCFWGAERCFWNTPGVYSTAVGYAGGSTPNPTYEEVCSGLTGHTEVVLVVY
                     DPQKVSYAELLKVFWESHDPTQGMRQGNDVGTQYRSAIYTFDDEQAAAARASADDYQK
                     ALSAAGFSEITTEIRSAPAFYYAEPYHQQYLAKVPNGYCGLGGTGVGCNVAPLVKAV"
     gene            8683..>9746
                     /locus_tag="AC16PP_12095"
     CDS             8683..>9746
                     /locus_tag="AC16PP_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HIR6482416.1"
                     /translation="MKIIHFAAPSATASSPSRRATRLFPFGIQGAALIVVLAASMVGC
                     QSMPLDYAGLNDKIANGDQVAVGELRRAFLDLEDLPERMERLRDLELQAAQLFEDEPL
                     KLGSIGTAILDTYYGSLTGHFALSRFYEFVGSEEAAVPHRQWQARIREDMEASADGSR
                     ESPYPAVTDVEAQMYAVSLGLSPVGSIYQTGEAFPYTLLIQARPADGPLRNLYFDLGG
                     WYESMRRQFPDENEFSPFALIGFQARRGDTAAQVAVGAFLATQGRGDEAVDWLRAASR
                     TGNLLANSLLARIFWEEANKDGEDDARQAALDEVLENYLHAVALGSADAMYALGVLYM
                     NEHYGAENRDSGVPLLRQAA"
BASE COUNT         1774 a         3274 c         3103 g         1595 t
ORIGIN      
        1 tcgccatgtc gatggctgcg tgatcgtcgt cccgggacgc tcccagcagc tgctcggtaa
       61 ccgccatggg cgcggacgtg cccagcagcg cgagccccag gcagcggtcg ggaaagagcc
      121 gggcaaaggc ctgcccaacc agggagccca ggctgtgacc gacgacgacg gcctccgcca
      181 cgcccagcgc gtcgagcagc tcggcaagcc aggatgcaag ggctggtaca gaagccaacg
      241 gcgccccctt cgatcgcccg tgggcgggaa agtccggcgc caccaccgcg aaaccgtgtc
      301 gcgcgaagta acggctgggc atgacccaga cgctgtgatc gaagcccgcc ccgtgaagaa
      361 acaccacgca gggccgccgt gcgtcgaacg gcacgctgcc cgtgcccgcg aaggcgcagt
      421 gaccgccgac ttccaccctc atgtggacac tccggccgcc cgctcggccc gcttcaacgc
      481 cccccgcaga tcgtcgatca ggtcggccgg atcttcgagc ccgaccgata cccgaatgag
      541 cccttcgccg atcccggccg cctcgagggc ctttgcgtcc atgcgatggt gggtcgtgct
      601 ggccggatgg atgaccagcg acttcgcgtc cccaacgttc gccaggtgcg agaagagctg
      661 caggctttcg atgaacgcgc gcccggcttc ccgcccgccc ttgatgtcga atcccatcac
      721 cgcacccgct ccgcgcggca gcagcgtctt cgccaggtcg tgatcggggt gcccggcaag
      781 atcgggatgg ttcacggcgg tcaccatggg atgcccggct agaaaatccg caattgctcg
      841 agcattggcc acgtgccggt ccatgcgcag cggcagcgtt tcgaggccct ggagaatctg
      901 aaaggccgtc atcggcgcca tgcacgcacc gaaatctctc agaccctcct tccgggcgcg
      961 catgatgaac gcttccgggc cgaactcttc ggcaaatacc agcccgtgga aaccgtcgta
     1021 gggttgggac agggttgaaa acctcgccga ggcctcccag tcgaaggttc cgccgtcgac
     1081 gatcaggccg ccgataacca ctccgtggcc gcttaagaac ttggtcgcgg aatgcacaac
     1141 caggtccgcg ccgtgatcga gagggcggaa caggtacggg gtggtgaacg tggcgtcgat
     1201 catcagcggg agccccgctg cgtgcgccgc gtcggcaacg gcggggacgt tcatcacctc
     1261 gagccccggg ttgccgagga tctcaccgaa caccaggcgg gtctcttccc gcacggcgtc
     1321 agcaaacgcc tgggggtcgc gaggatccac gaaggtggtg ctgataccga atcgcggcag
     1381 ggtatacgcc agaagattgt gggtgccgcc atagagagat cgcgaagcaa cgatgtgatc
     1441 tcctgcgccc agcagcgtgg ccaccgcaag atgcatggcg gcctggccgc tggccgtggc
     1501 gatggcgcct acgccaccgt ccagcgcagc gatgcgctcc tccagcacgg cattggtggg
     1561 attcgaaagc ctcgagtaaa cgtgcccggc gcgctcgatg ttgaaaagcc cggcggcgaa
     1621 gtccgagtcg gggaagacga aagcggcgct ctgatagatg ggcgtcgccc gggcgcccgt
     1681 tgcaggatcc ggtcgcgcgc cggcatgcag cgccagagta tcgaatccgg taggtcgggg
     1741 agcctggtgt tttgtgcctg ccatgatggg cgccttccgc ataggagggc gacgaggcta
     1801 accgagcccc acgggcagtg gcaatccgtt ctccgggccc ggaccctagc tcaggtagtg
     1861 cgcgcgcagc gcgtcgacat caccgcgggt gagcccgatg gcgcgctcca ggtaggcttc
     1921 caggcttccg caggcgctct ccatctcctc cagagcggcc atgatgtact cttcgcgaac
     1981 ccccgacagc acccgggccc cttcgacgtc gatgttgtcg tgacccagat tctggcggat
     2041 gcgcggcagt atgaacgcct cgaaatccat cacatcgttg gtgagcatgt agtcgtggat
     2101 gacggtttcc cggggcacgc cgagcgccag caggatcagc gccaccccga cccccgtgcg
     2161 gtcctttccg gcggtgcagt gcacgaggaa cccgcccggg gcatgctcga gcagcgcggc
     2221 aaacatcgac gagtaaccct cgacgtgatc gcgggcgagc tcccgcgtga tggtgcgcat
     2281 gaagtcgacc cgttggctca cgtccacgta accagcccgc agcgagtccc gcagctgggt
     2341 cgcgctgccc gggtccatgg gaatctcgac ccggcgggag acgtgatgcg gcacgggcgt
     2401 cggatccacc tcccgctcgt cgggccgcct gagatcgcag atcacgtcga tgcccagctc
     2461 cgcgaactgc gccagcgctt cctggctcag gctggcaagc tgaccgcaac ggaacagcaa
     2521 cccacgacga accgtcgcgc cgtcggcggc ggcgtagcca ccgaaatcgc gaaagttcac
     2581 cgcgcccgtg aggtcgacga ccctatccat gcaaggttcc cgtggcagcg tgaagaggcg
     2641 gcgagcttac cagaagcgca agccgagatg gcagcctgcc gccgctcagc ccgcccgttc
     2701 gtgattcagc gcgctcacct gctcgttgag cgtccagtag tcgtagatca cgccgatccc
     2761 gaacaggccc acggtaaaga gatagatgat cccggtcatc cacttgccct gataaaagcg
     2821 gtggacgccg aaaaaaccca gaaacgtcag cagcacccag gccaccgtgt agctcttctc
     2881 gccctgctga tagcgccggt cggcggacgc ctccaggttg ggcatgagga tcaggtcgat
     2941 gagccagccg atgaggaaca ggcccagggt gaagaagtag atggtcccgc ttacgggccg
     3001 gccgaaatag aagcggtgcg cgcccaggaa cccgaatatc cagaggatgt atccgaccgc
     3061 aaggctgtgc gtgttgttca tgcgggcctc cagcagatca ggcgaaagat gcttcacgtt
     3121 gccccgaacc ggcgccgccc gtcaacggtg acgtgccgtg agctccgcaa tgccccggat
     3181 gacctcgggc ccgaccggct ccggcagatc gtgcccgatg ccctcgagca ccaggagtct
     3241 ggaccgctcg agcgcctagt gggtatccac gccgccctcc acggggacga gggggtcgtc
     3301 gctgccgtga acgaggaaag ccgcgacgtc gagctgctcc ggcgccggcc accggttacc
     3361 ggccgcggcg atggcgatgc cggcgatctg gcgcgcaaaa ccttcggggt agaagctgcg
     3421 gtttaggtaa gaggggatcg gcgattttag aaccagctgc gagattcgca agctgcctca
     3481 caacatcgga ttgcacgccg ggacgctctc ggagtagggt tttctccacc cgacaaggaa
     3541 cgtcccgttg cagcagctgc ccgacccagt ccggatcccc accaacgaca tcgagctcga
     3601 ggttttcgag caaggcgcgg gcgcgccagt ggtgctgtgc cacgggtggc cggagcacgc
     3661 ctactcctgg cgacaccaga tgagccccct ggctgaggcc ggctaccacg tgatcgtgcc
     3721 caaccagcgc ggttacgggc actcgagccg accgcagaag gtgaccgact acgacatcac
     3781 ccatctcgcc gcagacctca ccggcctgct cgatcatttc gggtacgacc gggcggtatt
     3841 ctgcggccat gactggggcg ccatcgcggt atggaacctg gccatgctgc acccggacag
     3901 ggtggccggg gtgatcaact tgagcgtacc tctgctgcgc cgggggccta cggactgggt
     3961 ctcgcactgg gaaagcctcc tcggcgaaga tttctacatc gttcacttca accgccggcc
     4021 cggcgtggca gacgcggtct tcgaggaaaa cccgcgacgc ttcctcggca acctgtaccg
     4081 tacccatcaa tggcaggagc cagcagccga gccccgatcc ggcatgccgc tgatcaacct
     4141 cgcacgggca aaccggccgc gcggcaggcc gctgatgagc gacgccgagc tggacgtttt
     4201 cgtacgtgcc ttcgaacact ccgggttctc cggcggcatc aactggtacc ggaacttcgc
     4261 gcgcaactgg aagctgaccg ctgacgtgcc cgagcacgtt ccgcacccgg cgatgatgat
     4321 ctatggccgc tacgacatcg tgcccgtgtt cgacaagctc agggacttcg ttccgctggt
     4381 ggaaacccac gtgctcgact gcggccactg gatccagcag gaactgcccg gcgagaccaa
     4441 tcgcctgatg ctggactggc tcgagcgaaa ctatccttcg aaccgacgac ggagcagcca
     4501 atgaacgcca tcaagggctt gaaccacgcc gcctatcgat gccgcaactc tgaggagacc
     4561 cggcagttct acgaggattt cctgggattg cccctggtcg acgccctgcc catcggcact
     4621 accaagaccg gccgcgccgc ccaggtgctg cacacgttct tcgccatgga cgacggctcc
     4681 tgcgtcgcct ttttcgagga gccggacgcg gcgttcgact tcaaggatca gcgcgacttc
     4741 gatctgcatc tggcactgac cgtcgacatg gagaccctcc acgagatgtt cgagcgggga
     4801 aaggcgcagg gcatcgagac gcgcggcatc tcagatcacg ggttcattca ctcgatctac
     4861 ttccgcgatc cgaacggcta cgtggtcgag ctggcggcac ccgtggagct gcgaagcggc
     4921 gctagcgcgg atgggcggcg gctgcgcgcc cggcaggttc tcgacgagtg ggaggcacga
     4981 cggccctagc tttcccgatg ccgcttgccg ttcacgcggc tcgagaccgc tgcaggccgg
     5041 cgccgcgtca ggacgggatc atgaagctcc cgctggcctc cgcgaccacc tcctcggtgt
     5101 ccgtgcgaat cactttgcct tccatgatcg cgaggcggcc cttgcgcttc aggcagcggc
     5161 cttccagacg caccggcgtg cccaccggca gctgcgcccg gtagcggacg tccgcccggg
     5221 cagtgaagca ctgctcgccg cgcagccaga tccagttcgc catgacgtcg tccagcaggc
     5281 tgaagatgat tcctccgtgt gctaccttgt cgtagccgac gtgctcttcg cccggcgtga
     5341 attccgccag gcagacgtcc tcctcgaggc gaaagcgcac gtggagaccg atggggtttc
     5401 cgggcccgca gacgaagcag cggttagccc gactttctaa cgacatgctg aatttagagt
     5461 tgcccgaccc agccggtatg atagcccaag tgcacgacct gaatttaatt cgatcccgcc
     5521 tgtccgagca ccgcgcggaa gagtttgcgc cggagcccga catgcgggag gccgccgtgg
     5581 ccgtcatcct gcgtgaggtc tccagccggc cggccgagat cctgttcatc aagcgggcgg
     5641 agaagcccgg agacccctgg tcggggcaca tggcttttcc aggcgggcac cgggacccgg
     5701 aggacgagag tctcagagcg gccgcagaac gggagactct ggaggagatc gggctggacc
     5761 tcgattcggc tcgctacctg ggtccgctga accagcagcg cgccgtggcc cgcggccgcc
     5821 ccctcaacat gctcatcgcg ccccacgttt tcgagatcgc cggggaccct tcgttcacca
     5881 tcaactacga ggtggcacag gtggtctgga ccccactggc accgctcgta tcaggggcgc
     5941 ttcacgatac cgagacccgt ccgctggcag gcacgccgac ggtgttcaac ggctaccgcc
     6001 tgccccaggg acactttgtc tggggtctca cctaccggat ggtgaagtcg ttcttcgccg
     6061 ccatcgaccc gggctgggaa cctccgccgg aggtctagcg agcgccgctg acgcctaaac
     6121 gtccatgccg tggaagttgg cgatggccag gcctgcggcc acgctggtga acggatcgtg
     6181 ctccacgacc cgcccgggaa atcgatcctc cagaatgcgc cgcaccgcgg gaatcaggct
     6241 ggagccgcca gtgcacagca cgatgtccac gtccccatgg acgagcccgg cgagcgccac
     6301 cgtttcgtcc agcgcttgct ccgcctgttc caggaggtcg ccgatcagcg attcgaaacc
     6361 cgcacgggtc aaacgcacct ccacgtcgat ctcggatatg tccagcaccg cttcttcccg
     6421 agccgaaaga tctgctttga agtccttgat ggtctgaaac accacgtagc tcaagttctg
     6481 ctggatcagc tcccgaagcc ggcggaactt gcgcgcgccc ggaccgccct gccctatgca
     6541 ctccaagacc ggcgtggtgt agcggttctg gttgagcgtg taggtcaccg cccagttgac
     6601 caggagctcc tggtaatcct cgaaggggaa gacggtttcg atctcccggt ccataccccg
     6661 ccggcgccac gtctcgcctt tgccgagcag cgggaagagc agctcgcgaa acaggcgctg
     6721 atcaatgtgg tcgccgccca gcgcgatacc atgggtggtg accacctcga aatcgccggc
     6781 agcccgccgc agtatgcaga aatccagcgt gccgccgccg aaatccaggg tgagcagatg
     6841 ctcaccggca gcctcgggat gggcaaagag ataactcacg gcagcggcga tgggctccgg
     6901 ataaaagcgc tgctccgtga tgcccgcata cttgtaagcc tcccccagtc gcgccagagc
     6961 agtctggttg cggaactcgt cccggccttc gaagttcacc ggatggccca ggtgagcgta
     7021 gtcggccccg ctcaccgtcc gggagagagc cttacgcatg cgaagcagta tcggcgtgat
     7081 gagggcaacc aggcggaaag gatgatcgaa aaccatcagg cgcctcaccc ggggatcgcc
     7141 caggagccgc ttggtccccc ggaacagccg cccgtgcagg ccgctgtcga tcagcggctg
     7201 cccgtagatt ttctgggtga cggtttccac cggcgcgggg ttctccgaat ccccatgctc
     7261 cacgaaggcg ctggtctctg cgatcacctc tggaaccagc tccacggttc ggccggtatt
     7321 gtcggcgatg tactgattga tggccgcctg accggtaagc gtctgcagct cacggtcgat
     7381 gtaggtggcg gagggcatga tcggatcggc gccctccagc gcaacgagct gcagccgctc
     7441 gccgtcgtag gtggccgccg ccgagttggt cgtgccgaaa tcaatgccga cccccacgcg
     7501 catcagttgc cgccctctcg ttgccaattc atctccaacg cctcgtcccg gaaaccttgc
     7561 cggccggcgc aatcacgcaa gagtcacccg gatgtaagca cgccggcgga accgccccgt
     7621 caatcgcgag aggagatcgg caagtcgcat ctgccacccg taccggaggc gcagcgccac
     7681 gagcgccgcc tgggcgtccg cgccctcgat cagctccgcg cgtgcctgca gccactcccc
     7741 ctgcagcttg cccttgacgt cacaggcagc aacccgcgcg gtctggtcac ggcgcaaacg
     7801 tttgaccttt ccggcatcac ccgccgagaa gacgtagtaa acgcccggcg cttcgcccgc
     7861 cgagggagca aaccacaccg gcgtctcgac cgccgagccg ttcttcctga aggtagccag
     7921 attgaagtag cgtgcgtcgt cgagcgaggt gcccaccgac aatctccgcc gcgaagcaag
     7981 cggcggagtt tatcgcgaat tcaaaccgcc ttcacgaggg gcgccacgtt acagccgact
     8041 ccggtccctc caagaccgca atagccgttg ggaaccttcg caaggtactg ctgatggtac
     8101 ggctcggcat agtagaacgc cggggcagac cttatctccg tcgtgatctc ggaaaatccg
     8161 gcagcagaaa gcgccttctg atagtcgtcg gcggacgccc gcgcggcggc cgcctgctcg
     8221 tcgtcgaacg tgtagatcgc cgagcggtac tgggtgccca cgtcattgcc ctggcgcatg
     8281 ccctgggtgg ggtcgtgcga ctcccagaag accttgagca gctcggcgta ggaaaccttc
     8341 tgaggatcgt atacgacgag cacgacttcg gtgtgccccg tcagcccgga gcagacttcc
     8401 tcgtaggttg ggttcggcgt gctgccgccg gcatagccga cggccgtgga gtacaccccg
     8461 ggcgtgttcc agaaacaccg ctccgcgccc cagaaacaac caagagcgaa cacggcctga
     8521 gcgaggccct cgggaaacgg ttccttgagg ggggcgccgt tgacgtggtg tgccgccggc
     8581 acgggcatct cctcggctcg tcccggcaga gcctcggatt cactggggat acggagtttt
     8641 tgctcccaag ccatgtccaa ttgcctgtaa cattgaagga agatgaaaat catacatttc
     8701 gccgccccct ccgcaactgc cagttcgccc agccgccgag ccactcgcct cttccccttc
     8761 gggattcagg gggcggctct gatcgtcgtg ctggcagcat cgatggtcgg ctgccagagc
     8821 atgcccctcg actacgccgg cctcaatgac aagattgcaa acggggatca ggtcgccgtg
     8881 ggcgagctgc gcagagcgtt tctcgatctg gaggatctgc cggaacggat ggagcgtctt
     8941 cgcgatctgg agctgcaggc ggcgcagctg ttcgaggacg aacctctgaa gctgggatcc
     9001 atcggcaccg ccattctgga tacctactac ggcagcctga cgggtcactt cgcgctttcg
     9061 cggttctacg agttcgtggg ctccgaagag gccgcggtac cccaccggca gtggcaggcg
     9121 cgcattcgcg aggacatgga ggcgtccgcg gatggcagcc gggaatcgcc ttacccggcg
     9181 gttaccgacg tggaagccca gatgtatgcc gtcagcctgg gcttgagccc ggtgggctcc
     9241 atctaccaga cgggcgaggc gttcccctac acgctgctca tccaggcgcg gccggccgat
     9301 gggcctctgc gcaacctgta tttcgatctg ggcggatggt acgagtccat gcgccggcag
     9361 ttccccgacg aaaacgaatt ttcgccgttc gcgctgatcg gctttcaggc ccggcgcggc
     9421 gataccgcag cccaggtcgc cgtcggcgcg tttctcgcca cccagggcag aggcgacgaa
     9481 gccgtggact ggctgcgagc ggcctcgcgt accggaaacc ttctcgccaa ctcccttctc
     9541 gcccgaatct tctgggagga ggcgaacaag gacggcgaag acgatgcccg gcaggccgcg
     9601 ctggacgagg tgctggaaaa ctacctgcac gccgtcgcgc tcggctcggc cgacgccatg
     9661 tacgcgctgg gcgtcctcta catgaacgag cactacggag ccgagaaccg tgactccggg
     9721 gtgccgctgc tgcgccaggc agcgga
//
LOCUS       DBNHKV010000378         2286 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1471134, whole genome shotgun sequence.
ACCESSION   DBNHKV010000378 DBNHKV010000000
VERSION     DBNHKV010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1471134"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="AC16PP_12100"
     CDS             <1..228
                     /locus_tag="AC16PP_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482417.1"
                     /translation="FGLIDTIGALSPAVLDDVVQGGGEALRAMLAATPEPLAGVNLCA
                     SVNGHRDLAALCGRLTDCSGLHLSGATHPAS"
     gene            265..1584
                     /locus_tag="AC16PP_12105"
     CDS             265..1584
                     /locus_tag="AC16PP_12105"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6482418.1"
                     /translation="MKEQSRYDHLREKAVLAPTTPTRRRQRIPFDPQLDPYEIPLQDL
                     NVANPELFRAEAFWPYFRRLREEAPVHYCSESQYGPYWSITRYDDIMQIEKNHAVFSS
                     EFSLGGVTLMGTPSSGNDVPMFIQMDPPKHDAQRKAVAPKFTQRNLGDLEATVRERAG
                     RILDGLPRNETFNWVEHVSIELTAQMLATLFGVPQEERHQLIRWSNIISNADNPEVVK
                     EKSSYFEALAQCGDYFDALWRDRKSSAPTGDLVSMLAHGPDTADMNSQELLGNAVLLT
                     VGGNDTTRNSISGGLLALNQFPEQYDKLLENADLVRTMVPEIIRWQTPLTHMRRTALA
                     DTEFRGQLIGKGDKVVMWYISGNRDPDAVERPDEFIIDRRRPREHLSFGFGIHRCLGN
                     RLAELQLRVLWEEILKRCPRIEVVGEPVRASSVLFRAIQSLPVRIAA"
     gene            1657..>2286
                     /locus_tag="AC16PP_12110"
     CDS             1657..>2286
                     /locus_tag="AC16PP_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482419.1"
                     /translation="MAGQARSAAIVGIHEYPSRDVEGSVSPLQIKAESAARALADAGL
                     SWQDVDAIYDAGEAGMMSGLTIAEYLGLKPNVIDTTNVGGSSYEYHAAHAARDIAAGK
                     ARVALLTYGSTAHSNARAIGVGGRGGGGMHPADNMDAFAGMTLVANYAMVAQRHMYEY
                     GTTSEQLAEISVATRCHAMRNPEAVKAMEDLEFLDIRETTVEDVVGSRMI"
BASE COUNT          426 a          752 c          741 g          367 t
ORIGIN      
        1 ttcggcctga tcgataccat cggtgctctg tctcccgccg tcctggacga cgtggtgcag
       61 ggcggaggag aagcgctgcg agccatgctc gcggccaccc ccgagcccct tgcaggcgtc
      121 aatctctgcg cgtcggtgaa cggccaccgg gacctcgccg cgctctgcgg ccgcctgacc
      181 gattgcagcg ggctccatct ctcgggcgca acccatccgg cctcctgata tgatggcggg
      241 gtcggcgatt ggtcagcgaa ggtcatgaaa gagcaatctc ggtacgatca tctgcgggaa
      301 aaggcggtgc tggcgccaac gacgcccacg cgacgccggc agcggattcc cttcgatccg
      361 cagctcgacc cttacgagat cccgctccag gatctcaacg ttgccaaccc cgagctgttc
      421 agggcggagg cgttctggcc ctacttccga cggctgcggg aagaggcgcc cgtgcactac
      481 tgttccgaaa gccagtacgg accgtactgg tccataaccc gctacgacga catcatgcag
      541 atcgagaaga accacgcggt gttctcctcc gagttctcgc tgggcggggt aacgctcatg
      601 ggcacgccgt cctccggcaa cgacgtgccg atgttcatcc agatggatcc gcccaaacac
      661 gacgcgcaac gcaaagcggt agccccgaag ttcactcagc gcaaccttgg cgacctcgag
      721 gccaccgtgc gggagcgcgc gggcaggatt ctcgacggcc tgccccgcaa cgagacgttc
      781 aactgggtgg agcacgtttc catcgagctg accgcgcaga tgctcgcaac gctgttcggc
      841 gtgccccagg aagagcgcca tcagctgatc cgctggtcga acatcatcag caacgcggac
      901 aaccccgagg tggtcaagga gaagtcgtcg tatttcgaag ccctcgcgca gtgtggcgat
      961 tacttcgacg ccctctggcg cgaccgcaag tcgtcagcgc ccacgggcga tctggtgtcc
     1021 atgctggccc acgggccgga cactgccgac atgaactccc aggagctgct gggcaacgca
     1081 gtgctgctga ccgtgggcgg caacgacacc acccgcaact ccatcagcgg cggcctgctc
     1141 gccctgaacc agttcccgga gcagtacgac aagctcctgg agaacgccga tctcgtccgc
     1201 acgatggtgc cggagatcat ccgctggcag accccgctca cgcacatgcg ccgcacggcg
     1261 ctggcggaca cggagtttcg tggccagctg atcggcaagg gtgacaaggt ggtcatgtgg
     1321 tacatatcgg ggaaccgcga tccggacgcc gtggagcggc cggacgagtt catcatcgac
     1381 cgcaggcggc cccgtgagca cctttccttc gggttcggca tccaccgctg tctggggaac
     1441 cgtctggccg agcttcagct gagggtgctc tgggaggaaa tcctcaaacg atgccctcga
     1501 atcgaggttg tcggggagcc cgtgcgtgcc agctcggtgc tgttccgcgc cattcagagc
     1561 ctgcccgtgc gcatcgccgc ctagcccgca aggaccggcc ttggaccgtc ggcccgcacg
     1621 gcttagactg cgggcatcaa cctgggggag atagccgtgg ctggtcaagc cagaagcgcc
     1681 gccatcgtcg gcattcacga gtacccttcg cgcgacgtcg agggatccgt cagcccgttg
     1741 cagatcaagg cggaaagcgc tgcccgggcg ctcgccgatg ccgggctgag ctggcaggac
     1801 gttgacgcca tctacgacgc gggtgaggcc gggatgatga gcggtctcac catcgccgag
     1861 tacctcgggc ttaaacccaa cgtcatcgac accaccaatg tgggcggcag ctcctacgag
     1921 taccacgccg cccacgcggc cagggacatc gcggcgggca aggcgagggt ggcgctgctg
     1981 acctacggat cgacagcgca cagcaacgca cgggccatcg gcgttggcgg gcgcggtggg
     2041 ggcggcatgc atccggcgga caacatggat gcctttgccg gcatgaccct ggtggccaac
     2101 tacgcgatgg tggcgcagcg gcacatgtat gagtacggca ccacgagcga gcagctggca
     2161 gaaatatccg tggcgacccg ctgtcacgcc atgcgtaatc ccgaggccgt caaagccatg
     2221 gaggatctcg agttcctcga cattcgcgaa acgacggtgg aggacgtggt cggctcgagg
     2281 atgatc
//
LOCUS       DBNHKV010000379         4404 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_762210, whole genome shotgun sequence.
ACCESSION   DBNHKV010000379 DBNHKV010000000
VERSION     DBNHKV010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_762210"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="AC16PP_12115"
     CDS             complement(<1..262)
                     /locus_tag="AC16PP_12115"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6482420.1"
                     /translation="MRVLVTGGNRYIGRELVFELARRGHELTVINSHESPLPEGTRRI
                     HADRRLPGEFEAALSAHRDDFDAVFDNTAFTLADLEPMVELFR"
     gene            386..934
                     /locus_tag="AC16PP_12120"
     CDS             386..934
                     /locus_tag="AC16PP_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6482421.1"
                     /translation="MTQTNGGLPGLRGVDHIGITAPDFDQAADFLENVLGLEAFYQMG
                     PFGSEETDWMLDMLNVHPRAVIRRVKQFRCGHGPNIELFEYESPDQRREMPKNSDWGG
                     HHITLYVDDIDAAMDHLRAHGVRLMAGGGPPVDDGPEAGLRSCYFLTPWDLQMELISY
                     PDGKAYERGTDRRLWDPRDPAR"
     gene            complement(931..1320)
                     /locus_tag="AC16PP_12125"
     CDS             complement(931..1320)
                     /locus_tag="AC16PP_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482422.1"
                     /translation="MTIFIRVLAALPALLFLVMGIQWLFAPAEAAAGLEMPLLQGVAA
                     SAQIGDLGSFFFVNGVLMALGQLPGRSHLLYAPAFLIGGAGVFRIAAALLGHAPFAVD
                     FILAEAIMTTILLVAARRLGQPAAAAA"
     gene            complement(1345..2406)
                     /locus_tag="AC16PP_12130"
     CDS             complement(1345..2406)
                     /locus_tag="AC16PP_12130"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6482423.1"
                     /translation="MTTHDESTGPEGYRVAEVEAWIAENVQALKPPFEWTRLQGGHSN
                     LTYLLVDGDGNRAVIRRPPQGELLPKAHDMSREWALISALGPTPVPVPAALGFCESPD
                     VTGAWFYVMGFVNGHPLYNSDDTRQWVPREHRRKMAFSFIDVLADLHAVDPDEVGLGE
                     LGKKENYVGRQLKTWYRSWTSSIEAADYDDPRAHELQRYLLENIPEQGPARVVHGDYG
                     LHNCLVGPDCTIAAVVDWEISTLGDPLADLAYALNWWPDPTDTEPVLPEAATAPEGFP
                     TRTELAARYAERTGRDLSKLDYYVGFNRWKSAAIVHGVYARYCEGKKTADEGVDLDEM
                     RGRIDRSLMLSEQAIARLR"
     gene            complement(2454..2906)
                     /locus_tag="AC16PP_12135"
     CDS             complement(2454..2906)
                     /locus_tag="AC16PP_12135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR6482424.1"
                     /translation="MSERQLPEGLPDWIVEHIRLYREDPDKGHMWDSTALGGPGVLPT
                     LLLTTRGRRSGEPRILPLIYAEVQGGYAIIASKGGFPTHPAWYLNLVDEPECGVQVGQ
                     DEFRARARTADGEEREAIWAQMEGIYPPYRDYQAATERRIPVVVLERI"
     gene            complement(2988..4205)
                     /gene="aroQ"
                     /locus_tag="AC16PP_12140"
     CDS             complement(2988..4205)
                     /gene="aroQ"
                     /locus_tag="AC16PP_12140"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma subclass chorismate mutase AroQ"
                     /protein_id="HIR6482425.1"
                     /translation="MTLPGLSRRWWCVALCLTVPVCAWAGDPGVAELAHQRLALMQDV
                     AAHKWVNGLPVEDGAREAKVLESAEAAALRFGVDVSATRALFEAQIEAAKEIQRYWFA
                     RWALGSPPRSAPDLVGDLRPRLLALGDLILAGLAAAPGRAGSVPPLPGLSESSRRRLS
                     DALSAVEPYPSRLEQVRRSGVLRIGTTGDYPPFSHRVDDDGPLGGADVDLGRLLAHSL
                     GAEARFVPTSWPTLMDDLAAGRYDVAMSGVSRTAARERVAAFTRAYHVGGKTPIARCS
                     ERERFSSLAQIDRRGVRVIVNPGGTNELFVDNNLTLADKVLHTDNRSIFQALIEGRAD
                     VMITDRIEVRLQVRLHEGVLCATTPRNFNHQEKAYLLPRDAAWHAYVDAWLVSMLAQG
                     VVERTIARHLGED"
     gene            complement(4202..>4404)
                     /locus_tag="AC16PP_12145"
     CDS             complement(4202..>4404)
                     /locus_tag="AC16PP_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482426.1"
                     /translation="AAALAHVVTDPVPVPSQRTPLELRSTSIEGQQRRLLQAHEALLK
                     LSDANRDQFGNLVDNLREELEG"
BASE COUNT          770 a         1501 c         1435 g          698 t
ORIGIN      
        1 cgcggaagag ctcgaccatg ggctcgagat cggcaagcgt gaacgcggtg ttgtcgaaca
       61 cggcgtcaaa atcatcccta tgggcagaca gagcggcctc gaactccccg ggcaaccggc
      121 ggtccgcatg aatgcggcgg gttccttccg gcagcgggga ctcgtggctg ttgatgacgg
      181 tcagctcgtg accacggcgg gccagctcga agacgagttc gcggcctatg taacggttgc
      241 cgcccgtgac cagaactctc atctaggacc ctcccctgcg ttcgggctga agatagcaga
      301 ggccggccac agcacgccat gcacaccccc gggcgttgct gctaaccttg gcggtgtcgg
      361 caacgggagc aaaggggagc atgtcatgac ccagacaaac ggaggcctgc cgggactgcg
      421 cggcgttgat cacatcggca tcacggcgcc ggacttcgat caggcggcgg acttcctgga
      481 gaacgttctc ggcctggagg ccttctacca gatgggcccc ttcggctccg aggagaccga
      541 ctggatgctc gacatgctca acgtccaccc gcgggccgtg atccgccggg taaagcagtt
      601 ccgctgtggc cacggaccca acatcgagct tttcgagtac gagtctccgg accagcggcg
      661 cgagatgccg aagaacagcg actggggcgg gcatcacatc acgctgtacg tggacgacat
      721 cgacgcggcc atggaccacc tccgagccca cggcgtgcgc ctgatggccg gtggcgggcc
      781 gccggtggac gacggccccg aggcgggcct gcgttcctgc tacttcctga ccccctggga
      841 cctgcagatg gagctgatct cctaccccga cggcaaagcc tacgagcggg gcaccgaccg
      901 tcgcctgtgg gatcctcggg acccggcccg ctaggccgcc gcagcggccg gttggcccag
      961 tcgtcgggcg gccaccagca gaatcgtcgt catgatcgct tcggcgagaa tgaaatccac
     1021 cgcgaagggc gcatgaccca gcagggcggc ggcaatgcga aacacgcccg ccccgccgat
     1081 gagaaacgcg ggggcgtaga gcagatggct ccggccgggg agctgaccta gagccatcag
     1141 aaccccgttg acgaagaaaa agctccccag atcgccgatc tgcgcacttg ccgccacgcc
     1201 ctgcagcagc ggcatttcaa gacccgcggc agcttccgcc ggcgcaaaca gccactgaat
     1261 gcccatgacc aggaacagca gcgcgggcaa cgcggcgagc acccggatga atatcgtcat
     1321 gatcgtctcc ctagcattgg ccgttcagcg cagccgcgcg atggcctgct ctgaaagcat
     1381 gagcgaccgg tcgattcgac ctcgcatctc gtccagatcc accccctcgt ccgccgtctt
     1441 cttcccctcg caatagcgcg cataaacgcc gtgcacgatg gcggccgatt tccagcgatt
     1501 gaagccaacg tagtagtcga gcttggagag gtcgcgtccg gtgcgctcgg cgtagcgtgc
     1561 cgcaagctcg gtgcgggtcg ggaatccttc gggggccgtg gccgcttcgg gcaacacggg
     1621 ttccgtgtcg gtcgggtctg gccaccagtt cagcgcgtag gccagatccg ccagcggatc
     1681 gcccagggta gatatctccc aatccaccac cgcggcgatg gtgcaatccg gaccaaccag
     1741 gcaattgtgc aggccgtaat cgccgtgaac cacgcgggcc ggcccctgct cgggaatgtt
     1801 ctccaggagg tagcgctgca gctcgtgggc gcgcgggtcg tcgtagtcgg ccgcctcgat
     1861 ggacgaggtc caggagcgat accaggtctt gagctgacgg cccacgtagt tctccttctt
     1921 ccccagttct cccaggccca cctcgtcggg gtccacggcg tgcagatcgg cgagcacgtc
     1981 gatgaacgag aacgccatct tccgacggtg ctcccggggt acccattgcc gggtgtcatc
     2041 ggagttgtac agcgggtgcc cgttcacgaa gcccatgacg tagaaccagg cgccggtaac
     2101 gtccgggctc tcgcagaatc ccagcgcggc gggcacgggc accggtgttg gcccaagggc
     2161 cgagatcagg gcccactcgc ggctcatgtc atgggctttc ggcagcagct cgccctgggg
     2221 cggcctgcgg atgacggccc ggttgccgtc accgtccacg agcaggtagg tgaggttgga
     2281 gtggccgccc tgaaggcgcg tccactcgaa aggcggcttc agggcctgca cgttctcggc
     2341 gatccacgcc tccacctcgg caacccggta gccctccgga cccgtgctct catcatgcgt
     2401 cgtcatcgta tctccccccg cgcgacggcg gcgcccccgc gccttccggc ccctcagatc
     2461 ctttccagca ccaccaccgg tatccgccgc tcggtggcgg cctggtaatc ccggtagggc
     2521 ggatagatcc cttccatctg cgcccagatg gcttcccgtt cctcgccatc ggcggtgcgg
     2581 gcccgggcgc ggaactcgtc ttggcctacc tgaacgccgc attccggttc gtccacgaga
     2641 ttcagatacc acgccgggtg cgtcggaaac ccgcctttgg aggcgatgat agcgtagccg
     2701 ccctgcacct ctgcgtagat gagggggagg atgcgcggct cccccgacct gcgcccacgg
     2761 gtagtcagca gcagcgtggg cagcacgccc ggaccgccca gcgcggtgga atcccacatg
     2821 tggcccttgt ccggatcttc ccggtacagc ctgatgtgct cgacgatcca atcgggcaag
     2881 ccttccggca gctgccgttc actcatgtcc gcttcctccc ctttcagtcg gctcgatgct
     2941 ctcatggtgc accagagaag ccgtccccgc cagccgtcgc cctcgcgtca atcctcgccc
     3001 agatgccggg cgatggtgcg ctccaccacg ccctgagcga gcatgctgac gagccaggcg
     3061 tcaacgtagg cgtgccatgc cgcatcccgg ggcaggaggt aggctttctc ctggtggttg
     3121 aagttacggg gcgtggtggc gcaaagaacc ccttcgtgca ggcggacctg caggcgcacc
     3181 tcgatacggt cggtgatcat gacatccgca cggccttcta tcagagcctg aaagatagag
     3241 cggttgtctg tgtgcagaac cttgtcagcc agcgtcaggt tgttgtccac aaagagctcg
     3301 ttggtgcccc cggggttgac gatgacccga accccgcggc ggtcgatctg ggcgaggctc
     3361 gagaaccgtt cccgctccga gcacctggca ataggcgtct tcccgccgac gtggtacgcg
     3421 cgggtaaacg cggcaacccg ctcgcgcgcc gccgtccgag acaccccgct catggcgacg
     3481 tcgtagcgtc cagcagccag atcgtccatg agggtaggcc aggaggtagg gacgaatctg
     3541 gcttccgctc cgagcgaatg ggccagcaag cgaccgagat cgacgtccgc cccgcctaag
     3601 ggcccgtcat cgtcaacacg gtgcgaaaac ggggggtagt ctccagtggt gccgatgcgc
     3661 agaacgcccg agcggcgaac ctgctccaga cggctcggat agggctccac ggccgacagc
     3721 gcatcgctca gacgccgacg cgacgattcg ctgagccccg ggagcggcgg aaccgaccct
     3781 gcgcgacccg gcgccgcggc gagcccggcc aggatgaggt ccccgagggc aagcagccgg
     3841 ggccggagat ccccgacgag gtcgggggcc gagcgcggcg gtgagccaag ggcccagcga
     3901 gcaaaccagt agcgttggat ctccttggcc gcttcgatct gagcttcgaa gagcgcccgg
     3961 gtggcagaca cgtcgacgcc gaatcgaagc gcagcagctt ccgcgctttc gagcaccttc
     4021 gcctcgcgcg cgccatcctc gaccgggagg ccgttaaccc acttgtgagc cgccacgtcc
     4081 tgcatgagcg caaggcgctg atgggcaagc tcggcgaccc cggggtcgcc agcccacgca
     4141 catacaggaa cagtcagaca cagggcgacg caccaccatc gccgacttaa cccgggaagc
     4201 gtcagccttc gagctcctcg cgcaggttgt ccaccaggtt gccgaactgg tcgcggttcg
     4261 catcgctgag cttgagcagc gcctcgtgag cctggagcag acgtcgctgc tgaccctcga
     4321 tgctggtgct gcgcagctcc agcggcgtgc gctgggatgg caccgggacc gggtcggtga
     4381 ccacgtgggc aagcgcagcg gcca
//
LOCUS       DBNHKV010000380         4450 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1542067, whole genome shotgun sequence.
ACCESSION   DBNHKV010000380 DBNHKV010000000
VERSION     DBNHKV010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1542067"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..1838
                     /locus_tag="AC16PP_12150"
     CDS             318..1838
                     /locus_tag="AC16PP_12150"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0032787 - monocarboxylic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6482427.1"
                     /translation="MSVKAYDWVAYHARQTPEKLALVDLHSNRQFSYRDMQERTARLA
                     GAMRDGFGVRPGDRVAALLNNSSDCMEMNFACMKLGAIYVPLNWRLTVSELEYIVGDA
                     EPSLLIHEDVFSDEAEALKERCSVRALLETTASGTGNAYEAAIDAAAPLTGSAEITHD
                     DTWVIMYTSGTTGHPKGCMITYGMTFWNVVNLLNPHRLSEDMVNLITLPLFHTGGLNV
                     YGNPAVHMGGTNIVMRSFDPGETLRILQDAEYGITHMIGVPANYLFMSQHPAFAEADL
                     SRLRAMAVGGAPTSLELLKIYADKGNALQQAFGMTETSPLVTALTPANALDKPGSAGL
                     AVLHTEIRLVDEEAREITAPNVVGELWVKGPNVTPGYWRRPDANESSFTDGWLHTGDA
                     AYQDEDGFIYIVDRWKDMYISGGENVYPAEVENVIYQLPAVAEVAVIGVPHERWGETG
                     QAVIVLKEGESLSEEAVLKHCKDKLARFKQPSSVRFIDEIPHNATGKQLKRELREQ"
     gene            1914..2273
                     /locus_tag="AC16PP_12155"
     CDS             1914..2273
                     /locus_tag="AC16PP_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482428.1"
                     /translation="MAWHSAGCRVHRGAGATLYLQNLLNFDWEQTRSPAGAIIRLSTD
                     GSLRQSVPDAHVVGKFNPPVMTTADLALKFDPEYRKVAERFLADPDACQRQLKYTATP
                     VDLIFGSTAELRAIAAV"
     gene            complement(2364..3563)
                     /locus_tag="AC16PP_12160"
     CDS             complement(2364..3563)
                     /locus_tag="AC16PP_12160"
                     /inference="COORDINATES: protein motif:HMM:NF014954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6482429.1"
                     /translation="MNVQDDVPYTVEDLTAEWLSEGLKESGVLEGAAVTDFIMDVLGD
                     QVGFNGEVVIIAPTYSPPSDSAPASLVLKIPTASKNRIMGQTMGLYEKEIRFYRELQP
                     GLNIRTPRHYFSALDVADDPDVILERLRGMNRLPIWMIRGIVALANRFVSGNPRRYAL
                     LIEDLSGYRMGDQAAGCSDEDARHIVAAMARLHAQFWASETLDEKSWITPYAVTSRIM
                     QMRFLQGVDQYLCDEGGSLTPGRRDLIGWLRKNGIALTETFGDEPATLLHGDVRLDNI
                     CFDDATGEVVLFDWQTILAGPGASDLAYFISATLPGDADESQVDELIDVYHGELSQHG
                     VDVSRDRLRWQYEVGMLTMLHRVLPTTYPDQMELDAGRGLPLLQAWISRIYTRLEQID
                     HERILKI"
     gene            complement(3703..>4450)
                     /locus_tag="AC16PP_12165"
     CDS             complement(3703..>4450)
                     /locus_tag="AC16PP_12165"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6482430.1"
                     /translation="TVAGAAQPIPLEQLGLPFDPTYPHIAAGTNFRAHAEEVGLDEGP
                     FLFPKLTRATAWDARVPARTRLDYEVELCAVALGVYSADGPAPLGFVLCNDFTDRWAL
                     LKEIDLDTPMGQSGFPDAKGGAGMLPIGPFLLVPSRDADFHRRLEIGLAVNDRLRQRD
                     SAGLMIWSPHEIALTALENCEAPFVSTAGSHRLTPCDAIDEATLLLTGTPAGVAFHFL
                     NIWRPGSYLRSGDRVLAYGSHLGVLRNTIE"
BASE COUNT          866 a         1390 c         1439 g          755 t
ORIGIN      
        1 ggccaacagc gaaacgagcg tcgtctgggg atacttcacc cttactgcct tcctgaccaa
       61 cccgcaggcc tactatcctg gcaacaggat ggcggcggcg ccgctgcgct acgaagacgc
      121 gctgcagatc agcatgtacc tgaaccatgg ggaaaccttc tagctgacat cgacgcgtcc
      181 gactggccag gtccccgggc cgtttgggtg aggttgccgc gggcagcgaa cccgttcgcg
      241 gctcctgcgg cctgcggggc gcaggctgca agccatcgtc tatactcccc atcatcgaga
      301 gggaggggag cccgcagatg tccgtgaaag cctatgactg ggtcgcctat cacgcccggc
      361 agacgccgga gaagctggcg ctggtcgacc tgcattcgaa tcggcaattc tcttatcgcg
      421 acatgcagga acgaacggcg agactcgcgg gggcgatgcg cgacggtttc ggcgttcggc
      481 ccggtgaccg cgtggcggcg ctgctgaaca actcttcgga ctgcatggag atgaacttcg
      541 cctgcatgaa gctcggagcc atctacgtgc ctctcaactg gcggctgacg gtgtccgagc
      601 tggagtacat cgttggcgac gccgagccga gcctgttgat ccacgaggat gttttcagcg
      661 acgaggccga agcgctcaag gagcgatgca gcgttcgcgc cttgctcgaa accaccgcaa
      721 gcggaaccgg caacgcttac gaggcggcga tcgacgcggc cgcaccgctg accgggagcg
      781 cggagatcac ccacgacgac acctgggtga tcatgtacac ctccggaacc accggccacc
      841 ccaagggctg catgatcact tacggcatga cgttctggaa cgtggtgaac ctgttgaacc
      901 cccatcggct gtcagaggac atggtgaacc tgataacgct gccgctgttt cacacggggg
      961 ggttgaacgt ctacggaaat ccggccgtgc acatgggagg caccaacatc gtgatgcgct
     1021 cgttcgaccc gggcgagacc ctgcgcatcc tccaggatgc cgaatacggc attacccaca
     1081 tgatcggcgt gcctgccaac tatctgttca tgagccagca tccggcattt gccgaagccg
     1141 acctctcccg acttcgtgcc atggcggtag gcggggcgcc cacgtcactg gagctcctga
     1201 agatctacgc cgacaagggc aacgcgttgc agcaggcgtt cggcatgacc gaaaccagcc
     1261 ccctggtgac cgcgctgacg cccgccaacg cgctggacaa gccaggctcg gcagggcttg
     1321 cggtgctgca taccgagatc cgtctggtcg acgaggaggc ccgggagatc accgcgccga
     1381 acgtggtcgg ggagctgtgg gtgaaaggcc cgaacgtgac gcccggttac tggcgccgac
     1441 cggacgcgaa cgaaagctcg ttcacagacg gctggctgca cacgggggat gcagcctatc
     1501 aggacgagga tgggttcatc tacatcgtgg accgctggaa ggacatgtac atctcagggg
     1561 gtgaaaacgt ctatccggcg gaggtggaga acgtcatcta ccagctgccg gcggtggccg
     1621 aggtggccgt gatcggggtg ccccacgagc gctggggcga gactggccag gccgtgatcg
     1681 tgctgaaaga gggagaaagc ctgtcggaag aagccgtgct gaagcactgc aaggacaagc
     1741 tggcacggtt caagcagccg agctcggtgc gcttcatcga cgagatcccg cacaacgcca
     1801 ccggcaagca gctcaaacgc gagctcagag agcaatgagc cctcggccag ccgcgttcaa
     1861 ctcagttctg gtggccggcc gagttgggta actaaggtcg gctgttcatc cgcatggcat
     1921 ggcacagcgc cgggtgccgc gtccatcggg gagcaggggc gacgctgtac ctgcagaacc
     1981 tgctgaactt cgactgggag cagacccgca gccctgcggg cgccatcatc cggctctcca
     2041 cggacggatc gctacgccag tccgtgccgg acgcccacgt cgtgggcaag ttcaacccgc
     2101 ccgtaatgac caccgccgat ctcgcgctga agttcgatcc ggagtacagg aaagtcgcgg
     2161 agcggttcct ggctgatccg gatgcgtgcc agcggcaact caagtacacc gccacgccag
     2221 tggatctgat cttcggctcc accgccgagc tgagggcaat tgccgcggtc taggcctacg
     2281 acgacgggaa ggaacttttc ctgagcgact tcgtcgccgc ctgggtcaat ggcatgcggc
     2341 tggaccgctt cgacctgtag gcttcagatc ttaagaatac gctcgtggtc aatctgctcg
     2401 agccgggtat agatccgcga gatccaggcc tgcagcaacg gcaagccgcg cccggcatcg
     2461 agttccatct ggtccggata ggtggtgggg agcacccgat gcagcatcgt cagcatgccc
     2521 acctcgtact gccagcgcag ccgatcccgt gagacgtcta ccccgtgttg cgaaagctca
     2581 ccgtggtaga cgtcgatgag ctcgtcgacc tgagattcat cggcgtcccc ggggagcgtc
     2641 gcgctgatga agtaggcaag gtctgaagcg cctggaccgg ccaggatcgt ctgccagtca
     2701 aacagcacga cctcgccggt ggcatcatcg aaacagatgt tgtccaggcg aacgtctccg
     2761 tgcaggaggg tggctggttc atcaccgaac gtctccgtga gcgcgatgcc gttctttcga
     2821 agccagccga tgagatcccg gcggcccggc gtcagactcc cgccctcatc gcagaggtac
     2881 tgatcaacac cctgcagaaa acgcatctgc atgatcctgc tggtgacggc gtatggcgtg
     2941 atccagctct tttcatcgag cgtttcgctg gcccagaact gggcgtggag gcgagccatg
     3001 gctgcaacga tgtggcgagc atcctcgtca gagcaaccag cagcctgatc tcccatacga
     3061 tagccactca ggtcttcgat gagcagcgca tatcgacgcg ggttgccgct cacgaaccgg
     3121 ttggcgagcg cgacgatgcc gcggatcatc cagatcggca gccggttcat gccccgcaac
     3181 cgctcgagta tcacgtcggg atcgtcagcc acatccagcg cgctgaagta gtgacggggt
     3241 gtccggatgt tgaggcccgg ctgcagttcc cggtagaagc ggatctcctt ttcgtacaga
     3301 cccatggtct gtcccatgat gcggttcttt gacgccgtgg ggatcttgag gaccaggctg
     3361 gcgggagcgc tgtccgaggg cggcgagtaa gtcggcgcga tgatgacgac ctcgccgttg
     3421 aagcccacct ggtcaccgag cacgtccatg atgaaatcgg tgacggcggc gccctcgagc
     3481 acgccggatt ccttgagccc ctcagacagc cactcggcgg tcaggtcctc gacggtgtag
     3541 ggcacgtcgt cttgtacgtt catcgttcgg atccggagcg gagatcgctt ttcggagtac
     3601 ggactgcagg ggctcgcgca tccggctccg aaatgtcaca tccatcattc tagcaaccag
     3661 aagtcacgca gggtcaacac gacgccggcg cgttgagagc gctcactcga tggtgttgcg
     3721 aagcacgccc aggtgcgacc cgtaagctag cacccgatcg ccggatcgca gataggagcc
     3781 cggccgccat atgttcagaa aatgaaacgc gacaccggcc ggcgtcccgg taagaagcaa
     3841 cgtcgcctcg tcaatggcat cgcacggcgt gagccggtgg ctgccggcgg tgctgacgaa
     3901 gggcgcttcg cagttctcga gcgcggtcaa ggcgatctcg tggggcgacc agatcatcag
     3961 gcccgcggag tcgcgctgcc gcagacgatc gttgaccgcg aggccgattt ccaggcgccg
     4021 gtgaaagtcc gcatcgcgac tgggcaccag caggaatggc ccgatgggca gcatgcccgc
     4081 cccgcccttg gcgtcgggga atccggattg gcccatcggc gtgtccaggt cgatttcctt
     4141 caggagcgcc cagcgatcgg taaagtcgtt gcacagcacg aaacccagcg gggcaggccc
     4201 gtcggcggag tagacgccga gggcaacggc gcagagctcg acctcgtaat ccaggcgcgt
     4261 acgtgccggt acccgtgcgt cccacgctgt agcccgggtc agcttgggaa acaggaaggg
     4321 cccctcgtcc aggcccactt cctcggcatg ggcccggaaa ttggttcccg cagcgatgtg
     4381 cggataggtg ggatcgaacg gcaagccgag ctgctccaac ggaatcggct gcgccgcacc
     4441 ggccaccgtt
//
LOCUS       DBNHKV010000381        23328 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_620500, whole genome shotgun sequence.
ACCESSION   DBNHKV010000381 DBNHKV010000000
VERSION     DBNHKV010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 23328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 23328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23328
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_620500"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="AC16PP_12170"
     CDS             <1..1026
                     /locus_tag="AC16PP_12170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR6482431.1"
                     /translation="ESDPVKALFGFDAVVGNYASPYAPGSAYVLLHHVFGEANGQAGQ
                     WGHAIGGMGAITQAMLKEALARGVTVRTDADVRLVRVRNGRANGIVLEDGEEIGARAV
                     ASNLNPKLLFTRLIEAEALDDDFRARIARYRCGSGSFRINVALSQLPRFECAPPGQPE
                     LGTRSGIIMAPSLKYMERAYFDAREHGWSRKPIVEMLIPSTVDDSLAPPGQHVASLFC
                     QQFAPTLDGQSWAEARDAAAQTVFDTVEGYAPGFRQSILGAMVLSPEDLETEFGLTGG
                     DIFHGALSLDQLFSARPVLGHGDYRAPVRGLYMCGSGTHPGGGVSGAPGHNAAREIIR
                     DFRARRL"
     gene            1132..2790
                     /gene="oxc"
                     /locus_tag="AC16PP_12175"
     CDS             1132..2790
                     /gene="oxc"
                     /locus_tag="AC16PP_12175"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF006721.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HIR6482432.1"
                     /translation="MMATLDGTTLIARSLKQQGVEYMFGVVGFPVFGIAAAAQAEGIT
                     YIGMRNEQSASYAAQAAGYLTGRPQACLVVSGPGVIHAFAGLANAQQNCWPMILIGGA
                     SPTYQNGMGAFQEERQVELAKPYCKYAHGIDSVARIPYYVEQAIRTSLYGRPGPVYLD
                     LPDDIIRGTVEEDEVVSAAPVGPAPRSQARPEDVEAALCVLESAERPLVVVGKGMAWA
                     RAENEVREFIERTQLPFLASPMGKGVLPDDDPLSVGAARSTALKEADVVFLMGARLNW
                     IMHFGLPPRFAPDVRIIQLDIAAEEIGTNVPAEVALLGDGQAVAAQLNAALDNRQWFY
                     PADAPWRTLLREKASDNQAAVAPMIDAEAMPMNYYRAYRDIEAWLPEDAIIIGEGANT
                     MDIGRTQMANRSARNRLDAGTYGTMGIGLGFTIAAAVVHPDRPVVAVQGDSAFGFSGM
                     ELETACRYKLPIKVIVLNNGGIGAGIDELPEDGPIPPFSLTPGARYEGIMETLGGRGL
                     YVEDPKDLRGALDEAMAFDGPALVNVRIDPRAGRKQQQFGWLTT"
     gene            complement(2792..4051)
                     /locus_tag="AC16PP_12180"
     CDS             complement(2792..4051)
                     /locus_tag="AC16PP_12180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450 family protein"
                     /protein_id="HIR6482433.1"
                     /translation="MTAIDRTVFECLRFTTAVADRLVTGSRMSGPAFLENPYGEYARL
                     RARGEVIRNFSIGGWNVLGFEAVRDAMRDARFGVDVRKSPFVDRIVRLVSRGRPFRML
                     DEPTMLNIDPPDHTRLRRLARQGFLHRYVCALEPKIEGLVEECLATVQGGMFDLMDTL
                     ARPLPANVIADILGVPREDRARFQAWSELIAKNGRNIEYDALQQTDAVFNELIDYLAQ
                     ITDAKRADPGDDLISHLIAAEEDGDRLSADEIHATSALLLIAGHETTTRLIGNAMWLL
                     LSHPEQLEILRANPALMPNAVEEALRFEAPVQHMTRIALEDMDFHGVHIRKHQLCEVY
                     IGSANRDPQANPNPDVFDICRENIQHVGFGYGIHLCLGAQLARLETQMALKMLLERFP
                     RLALTGAPPAWGDSTFVRGLDELLLAA"
     gene            complement(4185..5693)
                     /locus_tag="AC16PP_12185"
     CDS             complement(4185..5693)
                     /locus_tag="AC16PP_12185"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HIR6482434.1"
                     /translation="METPLFLELLNLLASIFVFLLGLGLIAVIVIYIVDRTQTKHAVR
                     RNFPVIGRFRYVFEHLGEFFRQYFFALDREEMPFNRAERTWVYRAAKGEDTTVAFGST
                     RDLRPPGTVLFVNCAYPTLDEDAAPLGRVTIGPDCEHPYDCGSLFNISGMSFGAVSRP
                     AVTALSNGARMAGVWMNTGEGGLSSYHLDGGADIVFQIGTAKYGVRNERGELCDEKLA
                     EMAAHRQVKMFEIKLSQGAKPGKGGILPGNKVTDEIARIRLIPVGSDSISPNRHPEIE
                     NEGDLLDMIDRVRRATGKPVGFKTVIGAYGWLEDLFAEINARGPASAPDFITIDSADG
                     GTGAAPMSLIDYMGLPIRESLPLVVDLIEAYGLRERIRVVASGKMVTPGEAAWAVCAG
                     ADFVVSARGFMFALGCIQALQCNKNTCPTGVTTHDPDLQRGLDPVDKAERVRRYAQHM
                     HRQLGMIAHSCGVAEPRQLKRFHCRIVTQTGISVPLDELYPPLVRDPARLSA"
     gene            complement(5784..7406)
                     /locus_tag="AC16PP_12190"
     CDS             complement(5784..7406)
                     /locus_tag="AC16PP_12190"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HIR6482435.1"
                     /translation="MGPVRSAALSVGLADLTFDNRFTAALPGDPESGNSRRRQIQGAC
                     YSRVAPTPVAAPRLVAYSPETAELLGLGSADCLSAAFAEVFSGNRVLAGMDPHASCYG
                     GHQFGNWAGQLGDGRAINLGEVIGTRGEHWMLQLKGAGPTPYSRTADGLAVLRSSVRE
                     FLCSEAMHHLGVPTTRALSLVLTGEQVMRDMLYDGNPAPEPGAVVCRVAQSFIRFGHF
                     EILAARREPDLLRQLADFTIRTHFPHLAPGSAPLARDDYLAWFAEVRDATVEMVVEWM
                     RVGFVHGVMNTDNMSILGQTIDYGPYGWLENYDPNWTPNTTDAAGRRYRFSYQPGIAQ
                     WNLTRLAEAIYPLVEAAEPLEELLAGFEKRYVESWQSMMARKLGLRSPQPADVVLLNE
                     LDEVLQLAETDMTIFFRRLADLSPADPASDPVTVVREAYYLPDQLAGETLERIGNWLE
                     DYVARLRQEGVGNEERRQAMNAVNPKYVLRNYLAQLAIDDAESGDFALVGELLDLFRR
                     PYDEQPARERFAARRPDWARTRVGCSMLSCSS"
     gene            7645..9765
                     /locus_tag="AC16PP_12195"
     CDS             7645..9765
                     /locus_tag="AC16PP_12195"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HIR6482436.1"
                     /translation="MLRKTLHTIVWLGLLSGCSEGGPAETAGPVNTDTARIAAAQAEP
                     DQWLSHGGTYAEQRFSGLDEINDGNLDQLTLAWYFDFDTHRGQEGTPLMVDGVLYVTT
                     AWSKVFALDAATGRELWRFDPKVPGSAGYNACCDVVNRGAAFYQGRVYVGTIDGRLIA
                     LDASDGRVVWSTVTVPPDRPYTITGAPRAAKGKVFIGNGGAEYGVRGFVSAYDAETGE
                     LAWRFYTVPGDPELGPDGAASDEVLSSLAAPTWHGRAYLDYGGGGGTVWDAIVYDPEL
                     DQLYVGTGNGSPWVRALRSEGRGDNLFLSSVLALDPDTGAYLWHYQETPGETWDYTST
                     QQITLATMEIGGEPRRVLLHAPKNGFFYVIDRTTGKVVSAEKFVPVNWAQRVDLQTGR
                     PVEAPGARFVDAPFTAVVGGAGAHNWQPMSYSPGTGLVYIPAQEVPFVYTQEEGFAFK
                     PGEWNLGVVLAGARLPADEATRREIVAANQGRLIAWDPVNQREAWRVEHGAPWNGGTL
                     ATAGNLVFQGLTDRTFRAYRATDGKQLWSFDTQNVIITAPMSFAVNGEQHVAVMAGNG
                     GGLPLTLPTFSGPLQTPNGRLLVFKLGGTAELPPFEPEPLKPVIADVALTPDELDTGF
                     DLFGRYCAACHGLETLSAGVLPDLRSSPALASSDAWRSIVHDGALETRGMIGFQARMS
                     ADQVEIIRGYVANEARKLESDADG"
     gene            9784..11148
                     /locus_tag="AC16PP_12200"
     CDS             9784..11148
                     /locus_tag="AC16PP_12200"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014869595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR6482437.1"
                     /translation="MTPTERDLWLLPLGGCGEIGMNLNLYGHDGRWLMVDCGITFAEP
                     GESGPHIQMADPAFIASRRQRLCALIVTHAHEDHVGAVAHLWRQLQCPVYATPFTAEV
                     LRRKLAEARLLDDVPIHVVRSGDRLTLEPFDVEWVALTHSIPEPQALVIRTPAGSVFH
                     TGDWKLDPSPVIGADYDEARCRQLADEPIGTMVCDSTNAMVEGRSISEGELYAGLLDL
                     VSGARGRVIVGCFGSNVARLHTLIRVAADTGRFACLLGRSLHNYYAAARASGVWESDL
                     AFADAADIGYLPPDEVLVVATGSQGEPGAALDRLAADTHPLLNLEPRDTVLFSSRVIP
                     GNEPAVEKLIRRLERFGVTVIQDSELNMPIHASGHPARDELRDLYRWVRPDVALPVHG
                     EEAHMQANAELAREVGVPRQMTGRNGDLFMLAPVRGVRRDAARVGRLGLDNGRLVHLG
                     PPRR"
     gene            11156..11833
                     /locus_tag="AC16PP_12205"
     CDS             11156..11833
                     /locus_tag="AC16PP_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6482438.1"
                     /translation="MKIHADLSRRAVVKSPELPWVPSPLPGVDRRMLERDGGEIARAT
                     SIVRYAPESFFAQHEHSGGEEFFVLDGVFSDESGDYPAGWYVRNPIGSRHRPHTQSGT
                     TILVKLRQFQIGDDEYVRTDTNSGSWSPGEAEGVTIMALHAFGTERVSLARLATGTRM
                     PERAYPEGAEMFVLEGEIRDEHGVYPEGTWIRSPAGFSHRLRSPSGARLLVKTGHLPA
                     APASDVR"
     gene            12033..12422
                     /locus_tag="AC16PP_12210"
     CDS             12033..12422
                     /locus_tag="AC16PP_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6482439.1"
                     /translation="MTRFRTALALAVTLSAAAGCSERPADGPAAVRADDSPASLQSPP
                     AGQAAYAMACASCHDTGVAGAPRIGDDQSWSNRSPLWTAVLAEHAEDGYLGMPAMGGA
                     EELSERAVQDAVEYMLSATFPEAPPAD"
     gene            complement(12445..13953)
                     /locus_tag="AC16PP_12215"
     CDS             complement(12445..13953)
                     /locus_tag="AC16PP_12215"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF005565.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6482440.1"
                     /translation="MLSTPTEANIAAICESLGIEADAAEVAVYRDLVEGSIAGCALVD
                     EMPDDMADWTGGERTWERPAAADNPFNAWYVKTSVAGAPDGPLVGRTLAIKDNVLVGG
                     VPLMNGTSILEGYVPPVDATIVTRILEAGGTIAGKSVCEAYCFSGGSHTASSGFVTNP
                     HDASRAAGGSSSGSGALVAGGVVDMAIGCDQGGSIRMPASFCGVVGMKPTHGLVPYTG
                     ILGMGPAIDHVGPMTATVRDNALLLEVIAGPDGLDSRQYGAREQAYSAGLEDGARGLR
                     VGVLSEGFGREDSEADVDAKVRAAAERFGRLGADVSEVSVPLHAVASAICFSVIQSTV
                     ETIFGHDGALISRLDPAVASLVAAQHAWRDRADELPANVKVMLMSAEYLRREHGWEYV
                     AKGVNQIRRLRAAYDAALAEVDLLLLPTTPMKATELPAPDADPATVLGLAFAPLSNTM
                     AFNNTHHPAISVPCGMSDGLPVGMMLVGGHFEESLLYRAAFAFEQHEDWREL"
     gene            complement(13998..14762)
                     /locus_tag="AC16PP_12220"
     CDS             complement(13998..14762)
                     /locus_tag="AC16PP_12220"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6482441.1"
                     /translation="MDYLTVDEAKRATGLRLVLTQGVPGPWGESAKALFDLKGVAYHP
                     VAQKGGAANEELLAWTGHRNAPIAILDDEPPRASWLEILFLAERLGESPRLLPEDIDQ
                     RMLVIGLSSEICSPGGFAWQSRMFLLQGMAKAAGDQPENFPMLKDYSYSQEAADRAVA
                     SVKPILDRLARQLDMQAAAGSDYLVGGELTAVDVYWAFFSQLLVPLPEAICPMPGFLR
                     KSYGMLGTQLEEPAPASLLAHRDRILERHIALPLTF"
     gene            14890..16020
                     /locus_tag="AC16PP_12225"
     CDS             14890..16020
                     /locus_tag="AC16PP_12225"
                     /inference="COORDINATES: protein motif:HMM:NF015368.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003847 -
                     1-alkyl-2-acetylglycerophosphocholine esterase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6482442.1"
                     /translation="MHRDLMTSTTFRHLTHETVAAGVTGQEIEAAIDELFSRERGSCL
                     NAPLATHGGPFPVLIASPGDAGSRHNLESVCEHLASHGYAVIVPEHTGSSPFAQLGRD
                     PWLDETGPSAKQAEHVRLVREITDSRGAYGARAGFGQTYSPLRPHREQGTAERQLNDA
                     LHERVRDLDTALNWLAAANARGPWAGRLRADRAGLLGRSFGAATALRAACADERLACT
                     FAVAAPAWRNAPFLELRKPTFLLSGAEDDLLIGMASSQAGAKPTPENPHPALFEAFIR
                     SRAPAMWGLLANADHDAFTCAARYWWPGLKPGQARRALAPDETFELIEPQRAQAVQQL
                     TARAFFDCFLRMDATALARLDPATLAAAGLTFDTRNLEQVAR"
     gene            16017..16202
                     /locus_tag="AC16PP_12230"
     CDS             16017..16202
                     /locus_tag="AC16PP_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482443.1"
                     /translation="MTLAEITALISGGGHVEVELHAVDPMIYVAYLVSGNRRQPVTGR
                     ERVSLQAEALRSFQPRR"
     gene            16238..16714
                     /locus_tag="AC16PP_12235"
     CDS             16238..16714
                     /locus_tag="AC16PP_12235"
                     /inference="COORDINATES: protein motif:HMM:NF019473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HIR6482444.1"
                     /translation="MIDLSMAGNEDEALALALLKAMEDGDLAAKIEELCTDDFVWANS
                     GLPTLSGKTAVRAHMEAGGFSRQIPILAAMTSFSADVLHIASRAGVVFTERVDHHWDA
                     DGRDLMTPHIAGVMEIRDGRIAALRDFYDVACYAQTPTAPNPDHAMAGTSDKGELT"
     gene            16711..17256
                     /locus_tag="AC16PP_12240"
     CDS             16711..17256
                     /locus_tag="AC16PP_12240"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6482445.1"
                     /translation="MKRTVDAEHLIKPAKFAHFVLRVSDIEASIDWYATVLGMEMVHR
                     ADMLAFMTYDEEHHRLALVQTPVEAATPGAPGLDHVAYTLGSLGDLMATYKRLKAADI
                     LPVWPINHGLTTSLYYADPDGNRVEFQVENLDSVEDLQGFMRSDAFAANPIGVGFDPE
                     KLLQRYENGDPLEELLQQGSA"
     gene            complement(17320..18669)
                     /locus_tag="AC16PP_12245"
     CDS             complement(17320..18669)
                     /locus_tag="AC16PP_12245"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR6482446.1"
                     /translation="MAGSSREQWSSRFGFLMASIGFAVGLGNIWRFPYITGENGGGAF
                     VIVYLLCAFGIGVPILMAEVMIGRRGRLSPPGSMREVAVAEGRSRQWQWAGGLNLATA
                     FIIEVIYCVVAGWVLYYLYQAVTGGFVGVDAGSAQAGFAQLMDDTGTMLFWTVVGLVV
                     TGLIIYAGVEKGIERAVRILMPTLFGLLVLLAVYNVFAGGFGQALDYLLSADFSKLNA
                     ATVLIAVGQAFFSIGVALAGMMTFGAYLPRDVSIGQSVLVIVFADTLVALTAGLVVFP
                     AVFHFGLDPAGGPGLIFQTLPVAFGQMPGGQLVSILFFLLLSVAAVTSMVGLLEPLTS
                     WLEEHRGLSRHGSAVLVLGSVAALSVLSILSYNVLTGFRVFGRDLNGVFDYLSNQIML
                     PLGGFLIALFVGWFVSKATSRDELDIPDSRYFQTWHALIRYVVPPAVLAILVFGVMS"
     gene            18840..19430
                     /locus_tag="AC16PP_12250"
     CDS             18840..19430
                     /locus_tag="AC16PP_12250"
                     /inference="COORDINATES: protein motif:HMM:NF016269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HIR6482447.1"
                     /translation="MFRTHRTATLVTAILMMLISGSAAADKYDETVELFRNAGDSNEF
                     FAKSYGYAVFPTIGKGGFGIGGARGKGRVYVGGNHVGNTTMTQLTIGFQIGGQGFSQI
                     IFFQDERSFREFSTGNFEFGANASAVAITAGASASASTSGATAGASGGKKDATTAGTA
                     YYKGMKVFTIAKGGLMYEASIGGQKFSYRPAGGDQS"
     gene            complement(19484..20929)
                     /locus_tag="AC16PP_12255"
     CDS             complement(19484..20929)
                     /locus_tag="AC16PP_12255"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6482448.1"
                     /translation="MVDDSGTFPTAGVLGRAFRSGELSPVAVTRELLERIDRLNPALG
                     AVHRLCPERAMAEAAAAERAFSAGEARSPLQGVPYLVKDLFDVAGLPTTAGTRLLEDN
                     VAEADSTAVARLAAAGMVLLGKTVTVQFAYGGAGVNHDHGTPRNPWRQDHYLPGGSSS
                     GSAVAVAAGLAPLALGSDTGGSVRIPASLCGITGLKTTVGRISRAGVYPLSWSLDSVG
                     PLARSVEDAARVYQLLAGADPGDETTLGRPAGDVLAGLEDGLDGLKIGIPENVFWEEV
                     DAEVAEAVLASADVLRSLGARVERMAFPAADGARLLNPRGLVIAAEAYAVNRRFMDDH
                     YDELDPVVATRMIQGKGVAAADYIENLLQWQALRVEAMKQMAGVDALICPSTMIPATP
                     LADCDCDLDRYVERNLAYLRNTSIGNVLNLCGLSVPCGFTAAGLPIGAMICGSPFAEA
                     TVLRIGHAYQRATDWHQRVPPLDWAAGVQSS"
     gene            21059..21559
                     /locus_tag="AC16PP_12260"
     CDS             21059..21559
                     /locus_tag="AC16PP_12260"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6482449.1"
                     /translation="MAYTPEQLSDLECIRDAAKRYCRGVDRLDEALMKSAYWPEATDD
                     HGVFVGNAMEFAEMCMVSHLRWRSTSHCIFNHSIELDPDGIHARGEAYNVTFLFQDGA
                     DVLDTWYGRYLDVYEKRGDEWRILERVCVHEGTTTGPVSKMEIDAEKFRHGSFDRPSS
                     GRRIGP"
     gene            21577..22428
                     /locus_tag="AC16PP_12265"
     CDS             21577..22428
                     /locus_tag="AC16PP_12265"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR6482450.1"
                     /translation="MNVELELLASGYRLLEGPRTDADGALWFSDVYGGTVNRRDPDGI
                     IEVVVRDRRMVGGLLMHANGGFVMTGPSVVLWRDGQERVLLERDGVRFFNDLHADASG
                     RIYVGSIRADIANLDDEPDPPGEAYRIDLDGSVTELYGGVGISNGIGFSPDGRTLYHV
                     DSTSRGIWAHDLDDAGALSNRRHIAKPAFEKGIPDGLCVDTEGNLWVAHVQGGRVVVL
                     DPGGNHVDEIRVPASWVTSCAFGGSDYRDLYIVSADNTDDKALGGCVWRCRPGAAGHP
                     TPLARVG"
     gene            complement(22433..>23328)
                     /locus_tag="AC16PP_12270"
     CDS             complement(22433..>23328)
                     /locus_tag="AC16PP_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482451.1"
                     /translation="GEVLDASGYHNVELPNGRLMEAMEGDRVVGAFGARAATLEAVGD
                     WQSIQGDEFDALTPAGLFGRLTSVSPFLPRLMGMRYLGHVHANGVRQTMDGAVTPAPD
                     AALTVPIVLITGTSMSSGKTMSGRLIVRLLSRLGFRVVGAKLTGAARYRDVLSYEDAG
                     AAAVFDFVDEGLPSTVGDPADFAARLERLLSRIAGAAPDVVVAEAGASPLEPYNGKTA
                     IDVLGDRVRFNLLCASDPYAVVGVASAFERTPDLVAGGAANTTAGIELVSKLSGLTAM
                     NLLGKDCHEPLTRMLRDKLGL"
BASE COUNT         4134 a         7939 c         7598 g         3657 t
ORIGIN      
        1 gagtccgatc cggtcaaggc gctgttcggc ttcgacgccg tcgtcggcaa ctacgcgagc
       61 ccctacgccc cgggatcggc ctacgttctg ctgcaccacg ttttcggcga agccaacggc
      121 caggcgggtc agtggggaca cgcgatcggg ggcatgggcg ccatcaccca ggcgatgctc
      181 aaagaggcgc tcgctcgggg agtaacggtg agaacggacg ccgacgtgcg gctcgttcgc
      241 gtccgcaacg gtcgtgcaaa cggtatcgtg ctcgaagacg gggaagagat cggcgcccgg
      301 gcggtagcgt cgaacctcaa cccaaagctg ctgttcaccc ggctcatcga agccgaggcg
      361 ctggacgacg acttcagggc gcgtattgcc cgctatcgat gcggatcggg ttccttccga
      421 atcaacgtgg ccttgagcca gcttccgcgc ttcgagtgcg ctccgccggg ccagccggaa
      481 ctcggcaccc ggtcgggcat catcatggcg ccgagcctca agtacatgga acgcgcctat
      541 tttgacgccc gcgagcatgg ctggtcccgg aagcccatcg tcgagatgct cattcccagc
      601 acggtggacg actcgcttgc tccgccgggg cagcacgttg ccagcctctt ctgccaacag
      661 ttcgccccca cgctggacgg gcaaagctgg gccgaggccc gcgatgcggc tgcgcaaacc
      721 gtcttcgaca cggtggaggg ctacgcgccc gggttcaggc agagcattct cggcgccatg
      781 gtgctctccc cggaggacct cgagaccgag ttcgggctca cgggcgggga tatctttcac
      841 ggcgccctgt ccctggacca gctcttctcg gcccggccgg tgctcggcca cggcgactat
      901 cgcgcccccg tgcggggcct gtacatgtgc ggctccggca cccatcccgg gggcggggtg
      961 agcggggcac cgggccacaa cgccgcccgg gagatcatcc gcgacttccg ggcgcgccgg
     1021 ctctgaacgg acacacctcg aagcgagagg ggccggcggc cttgctatac tcggcccctc
     1081 ggggcacgcc agagccaccg cagagaagca gataacaaag cgagggaggg aatcatggca
     1141 accttagacg gcactacgct catcgcaagg agcctcaagc agcagggcgt cgagtacatg
     1201 ttcggcgtcg tgggctttcc ggtgttcggc atcgccgccg ccgctcaggc ggagggcatc
     1261 acctacatag gcatgcgtaa cgagcagtcc gcgagctacg ccgctcaggc ggccggctac
     1321 ctcaccggcc gtccccaggc atgtctggtg gtttcgggtc ccggcgtgat ccacgccttc
     1381 gccgggctgg cgaacgccca gcagaactgc tggcccatga tcctcatcgg cggcgcctcc
     1441 cccacctacc agaacggcat gggcgcgttc caggaagagc gccaggtgga gctggccaag
     1501 ccctactgca aatatgccca cggcatcgac agcgttgcgc ggattcccta ctacgtggag
     1561 caggccattc gaacgtcgct ctacggccgg cccgggcccg tctacctcga cctgcccgac
     1621 gacatcattc gcggcacggt ggaggaggac gaagtcgtca gcgccgcgcc cgtgggcccg
     1681 gcgccccgct cccaggccag acccgaggac gtggaggcag ccctctgtgt gctcgaatcg
     1741 gccgagcggc cactcgtcgt ggtcggcaaa gggatggcat gggcgcgcgc cgagaacgag
     1801 gtgcgcgagt tcatcgagcg gacccagctg ccgtttctcg cctcccccat gggcaagggc
     1861 gtgctcccgg acgatgatcc cctttcggtg ggagccgcgc gcagcacggc gctcaaggag
     1921 gccgacgtgg tcttcctgat gggcgcgcgc ctgaactgga tcatgcactt cggcctgcca
     1981 ccgcggttcg ctcccgacgt gcggatcatt cagctggaca tcgccgctga ggagatcggc
     2041 accaacgtcc ccgcggaggt ggcgctgctg ggcgacggcc aggccgtggc cgcccagctc
     2101 aacgccgcct tggacaaccg ccagtggttc tatcccgcgg atgccccgtg gcggacattg
     2161 ctgcgggaga aggcttcgga caaccaggca gccgtggcgc ccatgatcga cgccgaggcc
     2221 atgccgatga actactaccg ggcctaccgc gacatcgagg cgtggcttcc ggaagacgcg
     2281 atcatcatcg gggaaggggc caacaccatg gacatcggcc gcacccagat ggcgaaccgg
     2341 tcggcccgca accgtctgga cgcgggcacg tacggaacca tgggcatcgg cctgggcttc
     2401 accatcgcgg ccgccgtcgt tcatccggac cggcccgtgg tggccgtgca gggcgacagc
     2461 gctttcggtt tctccggcat ggagctcgag accgcctgcc gctacaagct gcccatcaag
     2521 gtcatcgtcc tcaacaacgg cggcattggc gccggcatcg acgagctgcc cgaagacggg
     2581 cccatcccgc cgttctcgct gaccccgggc gcgcgctatg agggcatcat ggagacgctc
     2641 ggcgggcgcg gcctttacgt ggaggatccc aaagacctcc ggggcgcgct ggacgaggcc
     2701 atggccttcg atggccccgc cctggtcaac gttcgcatcg atccgcgggc cggccgcaag
     2761 cagcagcagt tcggctggct gacgacctga atcaggcggc cagcagaagc tcgtcgaggc
     2821 cgcggacgaa ggtgctgtca ccccaggcgg ggggcgcgcc cgtcagggcg agccggggaa
     2881 aacgctccag cagcatcttc agcgccatct gcgtctcgag ccgcgcaagc tgggccccca
     2941 ggcaaaggtg gatgccgtag ccgaagccca cgtgctgaat gttctcacgg cagatgtcga
     3001 agacgtccgg gttcggattc gcctggggat cgcgattcgc tgagccgatg tacacctcgc
     3061 acagctggtg cttgcggata tgcacgccgt gaaagtccat gtcctccaga gcgatccgcg
     3121 tcatgtgctg cacgggcgct tcgaaccgaa gcgcttcctc gacggcgttt ggcatgaggg
     3181 ccggattcgc gcgcaggatc tcgagctgct ccggatggct gagcagcagc cacatggcgt
     3241 tgccgatcag gcgggtggtg gtttcgtgac cggcaatcag cagcagggcg ctggtggcat
     3301 gtatctcatc cgcggacagc cggtcgccat cctcttcggc ggcaatcaga tggctgatca
     3361 gatcgtcgcc cggatcggcc cgcttggcgt cggtgatctg cgcaagatag tcgatcagct
     3421 cgttgaaaac ggcgtcggtc tgttgcaggg catcgtactc tatgttgcgg ccgttcttcg
     3481 cgatcagctc cgaccaggcc tgaaaccttg cgcggtcttc ccgaggcacg cccaggatgt
     3541 cggcaatcac gttagccggc aggggacgcg ccagggtatc catgagatcg aacataccgc
     3601 cctggaccgt cgccaggcac tcctcgacca ggccctcgat cttcggctcc agcgcgcaga
     3661 cgtaccggtg gagaaagccc tgacgggcca gccgacgcag ccgggtgtgg tccggcgggt
     3721 ctatgttgag catcgtcggt tcatcgagca ttcgaaacgg cctgcctcgg ctcacgagcc
     3781 gcacgatcct gtccacgaaa gggctcttgc gaacatcgac gccgaaacgc gcatcccgca
     3841 tggcgtctcg gaccgcttcg aagcccagga cgttccatcc gccgatgctg aagtttcgta
     3901 tgacctcacc ccgggcgcgg agccttgcat attcgccata ggggttctcc aggaaagcgg
     3961 ggccgctcat ccggctgccg gtcaccaggc gatcggccac ggccgtggtg aagcgtaggc
     4021 actcgaacac cgtcctgtcg atggccgtca tggcgggact cccgttagaa gcgctggcct
     4081 gcgccgacac ctcattgttg cacccaccga tgccccgaca aggcttcgaa cgcgaacctg
     4141 tgcgccgcct cacagattgg aggcccagcg cggaccagct ccgactatgc ggacagccgg
     4201 gcgggatccc tgacgagcgg cggatacagc tcgtctaaag gcacggaaat tcccgtctgc
     4261 gtgacgatgc ggcaatgaaa gcgtttgagc tggcggggct cggccacacc gcaggagtgg
     4321 gcgatcatgc cgagctggcg gtgcatgtgc tgcgcgtaac gacgaacgcg ttccgccttg
     4381 tctacagggt ccagaccccg ctgcaggtcg gggtcgtgcg tggtcacgcc ggtggggcag
     4441 gtattcttgt tgcactgcag cgcctgtatg cagcccaggg caaacatgaa gccccgggcg
     4501 gagactacga agtcggcacc cgcgcacacg gcccaagccg cctctcccgg tgtgaccatc
     4561 tttccgctgg cgacgaccct gatgcgctcg cgcagaccgt acgcctcgat gaggtcgacc
     4621 acgagtggaa gactttctct tatgggaaga cccatgtagt cgatcaggct catgggagca
     4681 gccccggtcc cgccgtcggc gctgtcgatg gtgatgaagt ccggtgccga ggccggcccc
     4741 cgagcattga tttctgcaaa gaggtcctcg agccagccgt acgccccgat cacggtcttg
     4801 aagcccaccg gcttccccgt cgcccgcctg acgcgatcga tcatgtccag caggtctccc
     4861 tcgttctcga tctcagggtg acgattgggg ctgatggagt ccgaaccgac cgggatcaga
     4921 cgaatccgcg cgatctcgtc ggtaactttg ttacccggca ggatgccgcc tttccccggt
     4981 ttggcgccct gggagagctt gatctcgaac atcttcacct gccggtgcgc ggccatctcc
     5041 gcgagcttct cgtcgcaaag ctcgccgcgc tcgttgcgca cgccgtactt ggcggtcccg
     5101 atctgaaaga cgatgtccgc ccctccgtcg aggtggtatg aggacagccc gccttcaccg
     5161 gtgttcatcc acacgcccgc catgcgcgcg ccgttggaaa gggcggtgac ggccggtcgc
     5221 gagacggcgc cgaagctcat gccggagatg ttgaaaagag aaccgcagtc gtaggggtgc
     5281 tcgcaatcag gcccgatggt gacgcggcct aagggagccg cgtcctcgtc gagggtgggg
     5341 taggcgcagt tgacgaacag caccgtgccc ggcgggcgca gatcgcgcgt ggagccgaag
     5401 gccaccgtgg tgtcctcgcc ctttgcggcg cgatacaccc acgttctctc cgctcggttg
     5461 aacggcatct cctcgcgatc cagcgcaaaa aagtactgtc ggaagaactc tccgaggtgc
     5521 tcgaacacgt aacgaaagcg cccgatgacg ggaaaattgc ggcgaaccgc atgtttcgtc
     5581 tgggtccgat cgacgatgta gatcacgatc accgcgatca gcccgaggcc aagcagaaag
     5641 acgaagatgc tggcgagcag gttcaaaagc tcgaggaata agggcgtttc catgggcagc
     5701 ctcccgatag ccacggaccg agaatagagc accgaaagcg gacaatcgac ccgtcggacg
     5761 ccgcccggac tgggggcggg atatcaggag ctgcaggaca acatggagca gcccacccgc
     5821 gttcttgccc agtccggccg ccgggccgca aaccgctcac gtgccggctg ctcgtcgtag
     5881 gggcgcctga agagatcgag cagctcgcca accagagcga agtcgccgga ctcggcatcg
     5941 tcgatggcga gctgggccag gtagttacgc aggacgtact tcggattcac ggcgttcatt
     6001 gcctggcgcc gctcctcgtt ccccacacct tcctgccgca gccgggctac gtagtcctcg
     6061 agccagttgc cgatgcgctc gagggtctcg ccggcgagct gatcgggcag atagtaagcc
     6121 tcgcggacca ccgtcaccgg atcgcttgcc ggatcggccg gactcagatc ggccaggcgg
     6181 cggaaaaaga tggtcatatc ggtctcggcc agctgcagca cctcgtcgag ctcgttcaaa
     6241 agcacgacgt cggccggctg gggcgatctc agaccgagct tccgagccat catgctctgc
     6301 catgactcca cgtagcgctt ctcgaatccg gccagcagtt cctccagcgg ctctgcggct
     6361 tcaacgagag gatagatggc ctccgccagt cgggtgaggt tccattgagc tattcccggc
     6421 tggtacgaaa accggtaccg ccggcctgca gcatcggtcg tgttgggcgt ccagttcggg
     6481 tcgtagttct cgagccagcc gtaggggccg taatcgatgg tctggcccag aatggacatg
     6541 ttgtccgtgt tcatcacgcc gtgcacgaac ccgacacgca tccattccac gaccatctcg
     6601 acggtggcgt cacgcacttc cgcgaaccag gccagatagt cgtcccttgc aaggggcgca
     6661 gagcctggtg ccagatgggg gaagtgtgtt cgtatcgtga agtcggcgag ctgcctgagc
     6721 agatccggct cccgccgtgc cgcgagaatc tcgaagtggc cgaaccggat gaacgactgc
     6781 gccacccggc agaccacggc gccgggctcc ggcgccgggt taccgtcata aagcatgtcc
     6841 cgcatgacct gctcgcccgt gagcacgagg ctcagagccc gggtggtggg gacgcccagg
     6901 tggtgcatgg cttcggagca gagaaactcg cgcacggagg agcgaagcac cgccagaccg
     6961 tcggccgtcc tcgagtaggg cgtggggccg gcgcccttta gctgcagcat ccagtgctcg
     7021 ccgcgagtgc cgatcacctc gccgaggttg attgcccggc cgtcgccgag ctgacccgcc
     7081 cagttgccga actgatggcc cccgtagcag gaggcgtgag gatccatgcc ggcaagcacc
     7141 cggttgccgg agaacacttc cgcaaatgcc gcgctcaggc agtcggcgct gccgaggccg
     7201 agcagctcgg ccgtctccgg cgagtaggcg accagtctcg gcgcggcaac gggcgtcggc
     7261 gccaccctgg agtagcaggc gccctggatc tgccggcggc gggagttgcc ggattccgga
     7321 tcaccgggca gagcggccgt gaaccggttg tcgaacgtca gatcagccag accaacggac
     7381 agcgcagccg acctcacggg acccacgcgc gccgctcgga acgcagcacc tgtcctttcg
     7441 ctaccaatgc agacatcgac caaccccagg caaacccgcc aacgactggg gcaatcctac
     7501 cctaaagcca cagataggcg ccgccccgga ccccaccatg accttgcatg ccggcccagg
     7561 gctacatatg gttgcggcct ggtcggtgac caccaggatt ttctcgctcc tggcgcccgc
     7621 cgaactatca tctcaggtga caagatgctt cgaaagacac tgcacacgat cgtctggctc
     7681 ggcctgctgt ccggctgctc agagggcgga cccgccgaaa cggcgggacc cgtcaacacc
     7741 gacaccgccc gcatcgcggc ggcccaggcc gaacccgacc agtggctgtc ccacggcggc
     7801 acctacgccg agcagcgatt ctcaggattg gacgagatca acgatggcaa cctcgaccag
     7861 ctgacgctcg cctggtactt cgatttcgat acccatcggg gccaggaggg cacgcccctg
     7921 atggtggacg gcgtgctgta cgtgacgacg gcgtggagca aggtgttcgc tctggacgcc
     7981 gctacgggtc gggagctctg gcgcttcgac ccgaaggtgc ccggaagcgc cggttacaac
     8041 gcctgctgtg acgtcgtcaa ccggggcgca gccttctacc aaggcagggt atacgtcggg
     8101 acgatcgacg gtcgtctcat tgccctggac gccagcgacg ggcgagtggt ctggtcgacg
     8161 gtgacggtgc cgcccgacag gccgtacacc atcaccggcg ctccgcgagc cgccaaaggg
     8221 aaagtcttca tcggcaatgg tggcgcggag tacggcgtgc gcggctttgt cagcgcctac
     8281 gatgcggaga cgggcgagct cgcctggcgg ttctacacgg tgcccggcga cccggagctc
     8341 ggcccggacg gcgccgcgtc agacgaggtg ttgagctccc tcgccgcacc cacctggcac
     8401 gggcgcgctt acctcgatta cggcggcggg ggaggaacgg tctgggatgc catcgtctac
     8461 gatcccgagc tcgaccagct ctacgtgggc acgggcaacg gctctccgtg ggtgcgtgcc
     8521 cttcgctccg aaggtcgcgg cgacaacctc ttcctctcct cggtgctggc gctggacccc
     8581 gacacgggcg cctacctctg gcactaccag gagacgcccg gcgaaacctg ggactacacg
     8641 tcgacgcagc agatcaccct ggcgaccatg gagatcggcg gcgagccccg gcgcgtgttg
     8701 ctccacgcgc cgaagaacgg ctttttctac gtgatcgatc gcacaaccgg caaggtcgtc
     8761 tccgcggaaa aattcgttcc cgtgaactgg gcgcaacggg tggaccttca gaccggccgg
     8821 ccggtggaag cgccgggcgc ccggttcgtc gacgcgccct ttaccgcagt ggtcggcggg
     8881 gcgggcgccc acaactggca acccatgtct tacagccccg gcaccgggct cgtctacatc
     8941 ccggcccagg aggtgccctt cgtttacacc caggaagaag gcttcgcgtt caagcccggc
     9001 gagtggaacc tgggcgtcgt gctcgcgggc gctcgcctgc ccgcggacga ggcaacgcgg
     9061 cgggagatcg tggcggccaa ccagggccgg ctcatcgcct gggacccggt caaccagcgg
     9121 gaggcctggc gagtcgagca cggagccccg tggaatggtg gcacgctggc cacggccggc
     9181 aacctggtct ttcaggggct gacggatcgc acgttccgcg cataccgggc tacggacggg
     9241 aagcagctct ggtcgttcga cacccagaac gtgatcatca ccgcgcccat gagctttgcc
     9301 gtcaacggag agcagcacgt ggccgtgatg gccggaaacg ggggagggct gccgctgacg
     9361 ctccccacgt tcagcggccc gctccagacc ccgaacgggc ggctgctggt attcaagctg
     9421 ggaggcacgg ccgagctgcc gcccttcgag cccgagccgc tcaagcccgt catcgccgac
     9481 gtggcgctga cgccggacga gctcgacacc ggcttcgacc tcttcgggcg ttactgcgcc
     9541 gcctgtcacg gtctcgagac cctgagcgcg ggcgtcctgc ccgaccttcg ctcgtcgccc
     9601 gctctggcat catccgacgc ctggcgctcg atcgtccacg acggcgcgct ggagacgaga
     9661 ggcatgatcg gcttccaggc gcggatgagc gccgatcagg tcgagatcat ccggggctac
     9721 gtggccaacg aggcccgcaa gctggaaagc gatgccgacg ggtagacgcc ggctcgacaa
     9781 gccgtgacgc ccaccgagcg ggatctctgg ctccttccgc tgggcggatg cggcgagatc
     9841 ggcatgaacc tcaatctgta cggccacgat gggcgctggc tcatggtgga ttgcggcatc
     9901 accttcgccg agcccgggga gtccggaccc cacattcaga tggcggaccc ggctttcatc
     9961 gcctcgcgcc ggcaacgact ctgcgcgttg atcgtcaccc acgcccacga ggatcacgtc
    10021 ggcgcggttg cccacctctg gcgccagctt caatgtccgg tctacgcaac gccgttcact
    10081 gccgaggtgc tgcgtcgcaa gctcgccgag gcccggcttc tggacgacgt gcccattcac
    10141 gtggtgagaa gcggcgaccg tctgaccctg gagcccttcg acgtggagtg ggtggctctg
    10201 acacattcga tcccggagcc ccaggcgctg gtcatcagaa ccccggcggg tagcgtgttc
    10261 cacaccggcg actggaagct ggatccctcg cccgtgatcg gggcggacta cgacgaggcg
    10321 cgctgccggc agctggccga cgagccgatc ggcaccatgg tctgcgactc caccaacgcc
    10381 atggtggaag ggcgttccat cagcgaggga gagctctatg ccgggctgct ggatctggtc
    10441 agcggtgcca gaggccgggt gatcgtcggc tgcttcggca gcaacgtcgc ccggctgcac
    10501 acgctgattc gcgtagccgc cgataccggc cgcttcgcct gcctgctcgg cagaagcctg
    10561 cacaactact acgccgccgc ccgggcgtcc ggcgtgtggg aatccgatct ggccttcgcc
    10621 gacgccgccg acatcggcta cctgccgccg gacgaggtcc tcgtcgtcgc caccggcagc
    10681 cagggcgagc ccggcgccgc gctggaccgg ctggccgctg acacgcaccc gctgttgaat
    10741 ctggaacccc gggacaccgt cctcttcagc tcccgggtca ttcccggcaa cgagccggcc
    10801 gtggaaaagc tcatccgccg gctcgaacgt tttggcgtca cggtgattca ggacagcgag
    10861 ctcaacatgc cgatccacgc atcggggcat cctgcccggg acgagctgcg cgacctctac
    10921 cgctgggtgc ggccagacgt tgccctcccc gttcacgggg aggaggcgca catgcaggca
    10981 aacgcggagc tggcgaggga agtcggcgtg ccgcgccaga tgaccgggcg taacggcgac
    11041 ctcttcatgc tcgcgcccgt tcggggcgtg cggcgggacg ccgcccgggt gggccggctc
    11101 ggcctggaca acgggcgcct ggtccacctg ggcccgcccc gccggtgatg gcgccatgaa
    11161 gatccacgcg gatctgagcc gacgggcggt ggtaaaaagc ccggaactgc cttgggtgcc
    11221 ttctccgctg ccaggggtgg atcgacgcat gctcgagcgg gacggtggtg agatcgcccg
    11281 ggcgacgtcc atcgtccgct acgcgccgga atctttcttc gcccagcacg agcactcagg
    11341 cggggaagag tttttcgttc tcgacggggt cttttcggac gagtcgggcg actacccggc
    11401 cgggtggtac gtccgcaacc ccattgggtc cagacaccgt ccccacacgc agagcggcac
    11461 gactatcttg gtgaagctgc ggcagtttca gattggcgac gacgagtacg ttcgcacaga
    11521 caccaactcc ggcagctggt cgccaggaga ggccgagggc gtcacgatca tggcgctgca
    11581 cgctttcggc accgagcgcg tgagccttgc gcggctggct acaggtaccc ggatgccaga
    11641 gcgcgcctac cccgagggcg cggagatgtt cgtcctcgaa ggcgagatcc gcgacgagca
    11701 cggcgtctat ccggagggca cgtggatccg cagcccggca ggcttctctc accggctgag
    11761 gtcgccctcg ggcgcccggc tactcgtgaa gaccgggcat ctgccggccg cgccggcgtc
    11821 agacgtcagg taagccgcct tgcccacctg ctccaccaag acgtcccgag cgccttagag
    11881 atcggcgatg cctttgccgt ccttgaagtt gcaggcgtag aactttctga ccagtctggt
    11941 ttctgccgcg ccaggaggct cggtcaaacg cccgcttgcg cgccggccgc atgatactta
    12001 gcctttactc aacgaccagt cgagaaaccg ccgtgaccag atttcgcacc gcccttgctc
    12061 ttgccgtcac gctgtccgcg gcggccggct gctccgaacg tccggcagac gggcccgccg
    12121 ccgtccgagc cgacgactct ccggcgtcgc tccaatcgcc gccggccggt caggccgcgt
    12181 atgcgatggc atgtgcgagc tgccacgaca caggggtcgc gggcgcgccc cgcatcgggg
    12241 acgatcagag ctggtcaaac cgctcaccgc tctggacggc cgtgctggcc gagcacgccg
    12301 aggacggcta tctgggaatg cccgccatgg gcggtgccga ggaactcagc gagcgggcag
    12361 tgcaggacgc cgtcgagtac atgctctcgg ccacctttcc agaggcaccc ccggcagatt
    12421 gacccctgcg cctgaccggg gcgctcaaag ctcgcgccag tcctcgtgct gctcgaaggc
    12481 gaaagcggcg cggtagagca gggactcctc gaagtggccg cccaccagca tcatgcccac
    12541 gggcagcccg tcactcatgc cgcagggcac ggaaatcgcc gggtgatggg tattgttgaa
    12601 cgccatggtg ttgctcagcg gggcgaatgc cagcccgagc acggtggccg ggtcggcgtc
    12661 cggtgccggc agctcggtgg ctttcatggg cgtggtcggc aacaacagca gatccacttc
    12721 ggccaacgcc gcatcgtagg ccgcccgcaa ccggcggatc tgattgacgc ctttggccac
    12781 gtattcccag ccgtgctcgc gacgcagata ttcggccgac atcaacatca ccttgacgtt
    12841 tgcaggcagc tcgtcggccc ggtcgcgcca ggcgtgctga gcggccacca ggctggcaac
    12901 ggcaggatcc aggcggctta tcagagcgcc gtcatgaccg aatatggttt ccacggtgga
    12961 ctgaatcacc gaaaagcaga tggccgaagc caccgcgtgg agcggcacgg aaacctcact
    13021 gacgtccgcc cccaggcggc cgaagcgctc ggcggccgcg cgcaccttgg catccacgtc
    13081 cgcttctgaa tcctcgcggc cgaacccctc gctcagcacg cccacgcgca ggccccgcgc
    13141 accgtcttcc agcccagccg agtatgcctg ctcccgcgct ccgtattgcc gactgtccag
    13201 gccgtccggg ccagcgatga cctcgagcag aagggcgttg tcacgcacgg tagcggtcat
    13261 gggcccgaca tgatcgatgg ccgggcccat gccgaggatg cccgtgtacg gcaccagccc
    13321 gtgggtgggc ttcatgccca ccacaccgca gaacgaagcg ggcatgcgga tcgacccccc
    13381 ctggtcgcag ccgatggcca tgtccacgac gccgcctgca acgagagcgc cgctgcccga
    13441 cgaggaaccg ccagcggctc ggctcgcatc gtgggggttc gtgacgaatc ccgagctggc
    13501 agtgtggctg ccgccggaaa agcagtaggc ctcgcagacc gacttgccgg caatggtccc
    13561 acccgcctcg agaatccggg tcacgatggt ggcatcgacc ggcgggacgt agccttcgag
    13621 gatgctggtg ccgttcatca gcggcacgcc gcccacgagc acattgtcct tgatggccag
    13681 ggttcgcccg accagcgggc cgtcgggcgc gccggcgacg ctggtcttca cgtaccaggc
    13741 gttgaaaggg ttgtcggcag cggccgggcg ctcccacgtg cgttcacccc cggtccaatc
    13801 cgccatgtcg tccggcatct cgtccaccag ggcacacccc gcgatggaac cctccaccag
    13861 gtcacggtaa acggcgacct ccgcggcatc ggcctcgatg ccaagagatt cacagatggc
    13921 tgcgatgttt gcttcggttg gcgtgctgag catggtcttc tccctcccag agcggcgaag
    13981 ccgcgtgaaa ccaatcgtca aaaagtcagc ggcagcgcga tgtgccgctc gagaatccgg
    14041 tcgcgatggg ccagcaggct ggccggcgcc ggctcctcga gctgggttcc cagcatcccg
    14101 taagatttcc ggagaaagcc cggcatggga cagatggctt ccggcagcgg cacgagcagc
    14161 tgtgagaaga acgcccagta aacgtccacc gccgtcagct cgcctcctac caggtagtcg
    14221 ctgccggcag cggcctgcat atccagctgc cgcgcaagcc ggtcgagtat gggcttcacg
    14281 ctcgcgactg cccggtcggc cgcttcctgg ctgtagctgt aatccttgag catgggaaag
    14341 ttttccggct gatcgccggc cgccttggcc atcccctgga gcagaaacat gcgggactgc
    14401 caggcaaagc cgccgggaga gcagatctcg ctggaaagcc cgatgacgag catgcgctgg
    14461 tcgatgtcct cgggcagaag ccggggcgac tcgccgagcc gctcggcgag aaagaggatc
    14521 tcgagccagc tcgcccgcgg cggctcgtcg tcgagaatgg cgatgggcgc gtttcgatgg
    14581 cccgtccagg ccagcaactc ctcgtttgcc gccccgccct tctgcgccac gggatggtag
    14641 gcaacgccct tcaggtcgaa cagcgccttg gccgactcac cccaggggcc aggcacgccc
    14701 tgggtgagca cgagccgcaa acccgttgcc cgttttgcct cgtcaaccgt cagataatcc
    14761 atctcccctc taccccagcg atgtcgatcc caagttatac cccgaaaacg tcaggcatct
    14821 cgaccgcggc gcgacccggt ctgcgcgacc agggccacct acgccgacta cgtgttcggg
    14881 gatgccagcg tgcaccgcga cctcatgacc agcacgacgt tccggcacct gactcacgaa
    14941 accgtcgcgg ccggcgtgac cggccaggag atcgaagccg caatcgacga actcttttcg
    15001 cgcgagcgcg gcagctgcct gaatgcccct ttggcaacac acggcggacc ctttcccgtg
    15061 ttgattgcat ctcccggaga cgccggcagc cggcacaacc tggagagcgt ctgcgagcat
    15121 ctggccagcc acgggtacgc ggtgatcgtg cccgagcaca ccggcagcag cccgtttgct
    15181 cagctcggac gggatccctg gctggacgag accggcccct ctgcaaaaca ggcagagcac
    15241 gtccgcctgg tgagggagat cacggacagc cgcggagcct acggagcaag ggccggcttc
    15301 gggcagacct actcaccgct gcgaccgcac agggagcaag gcacggcaga gcgacagctg
    15361 aacgatgcgc tccacgagcg ggtgcgcgac ctggacaccg ccttgaactg gctggctgcc
    15421 gccaacgctc ggggaccctg ggcaggacgc ctgcgcgccg accgcgccgg tctcctcggc
    15481 cgctcattcg gtgctgccac ggctctacgc gccgcctgcg ccgatgagcg cctggcctgc
    15541 acgttcgccg tggcggcgcc cgcctggcgc aacgccccgt ttctcgaatt gcgcaagccg
    15601 acgttcctgt tgagcggcgc cgaagacgat ctgctgatcg gcatggccag cagccaggcc
    15661 ggggcgaagc cgaccccgga gaatcctcac cctgcgctct ttgaagcctt catccggagc
    15721 cgtgcgccag ccatgtgggg tctgctcgcg aacgccgacc acgacgcgtt cacctgcgca
    15781 gcccggtact ggtggccggg gctgaagccg ggccaggcgc gacgcgccct cgctccggat
    15841 gagaccttcg agctgatcga gccgcagcgg gcccaggccg ttcagcagct caccgcgcgg
    15901 gccttcttcg actgcttcct gcggatggac gcaacggcgc tcgccaggct ggatccggcg
    15961 acgctggcgg cggctgggct gaccttcgac acccgcaacc tcgagcaggt tgcccgatga
    16021 cgctcgccga gatcaccgcc ctcatcagcg gcggcggcca cgtcgaggtc gagctccacg
    16081 ccgtcgaccc gatgatttac gtggcttacc ttgtttcagg caaccggcgg cagcccgtca
    16141 cggggcgcga gcgggtgtcg ctgcaagccg aggccttgcg ctcgttccag ccccggcgtt
    16201 agggttcggg ctgagcgctt ctgggagaca gcaccggatg atcgacctct ccatggcggg
    16261 caacgaagac gaggcgctcg cgctggcgct gctcaaggcg atggaggatg gtgaccttgc
    16321 cgccaagatc gaggagctgt gcaccgacga cttcgtctgg gccaacagcg ggctgccgac
    16381 gctgagcggc aagaccgccg tgcgcgctca catggaagcc ggtggctttt cccggcagat
    16441 acccatcctg gctgctatga cgtctttcag cgccgacgtg ctgcacattg ccagccgggc
    16501 cggggtggtg ttcaccgagc gcgtcgatca ccattgggac gccgacggcc gggacctgat
    16561 gacaccgcac atcgcgggcg tcatggaaat ccgcgacggc aggatcgccg ccctgcgtga
    16621 cttctacgac gttgcctgct acgcgcagac gcccacggcc ccgaatcccg accatgccat
    16681 ggcaggcacc agcgacaaag gagagctgac atgaagagaa ccgtcgacgc agagcatctg
    16741 atcaagccgg ccaagttcgc gcacttcgtg ctgcgcgtat cggacatcga agcatccatc
    16801 gactggtacg ccaccgtgct cggcatggag atggtgcacc gggccgacat gctggcgttc
    16861 atgacctacg acgaggagca tcaccgcctg gccctcgtgc aaacgcccgt cgaggctgcc
    16921 acccccggtg ctccgggact cgaccacgta gcctacacgc tgggctccct gggcgatctc
    16981 atggctacct acaagcgctt gaaggcagcg gacatcctgc cggtgtggcc gatcaaccac
    17041 gggctgacga cctccctgta ctacgcggac cccgacggca accgagtcga gttccaggtc
    17101 gagaacctcg actccgtgga ggacctgcag ggcttcatgc gttcagatgc ctttgccgcg
    17161 aaccccatcg gcgtgggctt cgaccccgag aagttgctgc agcgttacga aaacggcgat
    17221 ccgctcgagg aactcctcca gcagggctcg gcctgacgca ggaacgcgcc gggccaacag
    17281 ggtcttcgat cgacccacgc cgcaggccgg cgcgcgtcgt cagctcatca cgccaaaaac
    17341 gagaattgcc agcaccgccg gaggcaccac gtagcgaatc aacgcgtgcc acgtctggaa
    17401 gtaccgggag tcgggaatgt ccagctcgtc ccgcgatgtc gccttgctga caaaccagcc
    17461 aacgaacagc gcgatgagaa agccgccgag cggaagcatg atctggttcg acaggtagtc
    17521 gaacacgccg ttgaggtccc gaccgaaaac gcggaatccg gtgaggacgt tgtaggagag
    17581 aatgctcagc acgctcaggg ccgccaccga acccagcacg agcaccgcgc tgccgtgccg
    17641 gctgaggccc cggtgctcct cgagccagga cgtgagcggc tccaagagcc ctaccatgga
    17701 ggtaacggcc gcaacggaga gcagcagaaa gaagagaatg ctgaccagct gcccacccgg
    17761 catctggccg aaagccaccg gcagcgtctg aaagatgagg ccggggccgc cggccgggtc
    17821 gagaccgaag tgaaacaccg cgggaaagac caccagcccc gccgtcagcg ccacgagggt
    17881 gtcggcgaac acgatcacca ggacgctttg accgatggaa acgtcgcgcg gcaggtaggc
    17941 gccgaacgtc atcattcccg ccagcgctac gccgatggag aagaaggcct ggcccacagc
    18001 tatcaggacc gtggccgcgt tcagcttgct gaagtccgcc gagagcaggt aatcgagggc
    18061 ctggccgaaa ccgccggcaa acacgttgta gaccgcgagc agaacgagga ggccgaacag
    18121 cgtcggcatg agaatccgca ccgcgcgctc gatgcccttc tccacgccgg catagatgat
    18181 gaggccggta acgaccaggc cgactaccgt ccagaacagc atggtgccgg tgtcgtccat
    18241 gagctgtgca aagccggcct gtgccgagcc cgcatcgaca ccgacgaacc cgccggtgac
    18301 ggcctgataa agatagtaga gaacccagcc cgcaacgacg cagtaaatca cctcgatgat
    18361 gaaggcggtg gcgagattca gcccgcccgc ccactgccac tgacgggagc gaccctccgc
    18421 cacggcgacc tcgcgcatgg atcccggcgg gctgagccgc ccccggcgcc cgatcatcac
    18481 ctcggccatc agtatcggca cgccgatgcc gaaagcgcag agcagataga cgatcacgaa
    18541 cgcgccgccg ccgttctcgc ccgtgatgta cggaaagcgc cagatgttac cgagacccac
    18601 ggcaaagccg atggaggcca tcagaaaccc gaagcgcgac gaccattgct cccggctcga
    18661 tccggccatg gcacagctct cgtcaagtag acgggctaat ctaacctcga agcccccctt
    18721 gatgcacggc cgaggtcggt tcaggcgcgg ttcagttgca gggattatcc tttcgaacaa
    18781 gctgggaagt gctatccttc cggccgctcg agaactgctt tcttcaagga gtcgtctgaa
    18841 tgtttcgaac tcatcgaacc gccaccctgg tcaccgccat cctgatgatg ctcatctcag
    18901 gcagcgctgc agcggacaag tacgatgaaa ccgtggagct gttccgcaac gctggcgaca
    18961 gcaacgagtt cttcgcgaag agctacggct acgccgtgtt ccccaccatc ggcaaaggtg
    19021 gcttcggcat cggcggcgcc cggggcaaag gccgggtcta cgtcggcggc aatcacgtgg
    19081 gcaacaccac catgacacag ctcaccatcg gctttcagat cggtgggcag ggtttcagcc
    19141 agatcatctt cttccaggac gagcgttcct tccgcgagtt ctccaccggc aacttcgagt
    19201 tcggagccaa cgccagcgcc gtggccatca ccgcgggcgc ctcggcaagc gcgtccacct
    19261 cgggagcgac ggcgggggca agcggtggca agaaggatgc gaccaccgcc ggcacggctt
    19321 actacaaggg catgaaggtg ttcaccatcg ccaagggtgg tctgatgtac gaagccagca
    19381 tcggcggcca gaagttctcc taccgaccgg ccggcggcga ccagtcctga tctgactacc
    19441 gctaccagct ggcatcgcgc gacggcccgt cgccgcgccc tcgtcaactc gactgcacgc
    19501 ccgccgccca gtccagcggt ggcacccgct ggtgccagtc cgtagcccgc tgataggcgt
    19561 gcccgatccg cagcaccgtg gcctcggcga atggagagcc gcagatcatc gcgccgatgg
    19621 gaagccccgc ggcggtaaat ccgcagggga cgctgagccc acagagattc agaacgttgc
    19681 cgatgctggt attgcgcagg tacgccagat tgcgttccac gtagcggtcg agatcgcagt
    19741 cgcagtcggc cagcggggtc gccggaatca tcgtcgaggg gcagatgagc gcatccaccc
    19801 ctgccatctg tttcatcgct tccacgcgca gggcctgcca ctgcaggaga ttttcgatgt
    19861 agtcggcggc agcgacgccc ttgccctgga tcatgcgcgt ggcgactacc ggatccagct
    19921 cgtcatagtg gtcgtccatg aaacggcggt tcacggcata ggcctccgcc gcgatgacga
    19981 gacccctggg attgagcagc ctggcgccgt ccgcggcggg gaaggccatt cgctcgacgc
    20041 gcgcgcccag agatctgagc acatcggcgc tggccagcac ggcttcggcc acctcggcat
    20101 ccacctcttc ccagaacacg ttttccggta ttccgatctt cagcccgtct aaaccgtctt
    20161 ccaacccggc cagcacgtcg cccgccgggc ggccgagggt ggtttcgtcg cccgggtcgg
    20221 ccccggcgag cagctgatac acacgtgctg cgtcctcgac gctgcgggcg agcggcccca
    20281 cggaatccag gctccagctg agcgggtaca cgcccgcccg gcttatcctg cccaccgtcg
    20341 tcttgagccc cgtgatcccg cagagggagg ccgggatccg gacggatccc cccgtatcgc
    20401 tgccgagcgc caacggcgcg agacccgccg ccaccgccac cgccgagccg ctgctggatc
    20461 cccccggaag gtagtggtcc tgacgccaag ggttacgcgg cgtgccgtga tcgtggttca
    20521 caccggcccc gccgtaggcg aactgcacgg tcaccgtctt gcccaggagc accatgcccg
    20581 cagcggcaag cctggccacg gcagtggaat ctgcctccgc cacgttgtcc tccagcagac
    20641 gggtccctgc ggtggtgggc agaccggcaa cgtcgaaaag atctttgacc aggtagggaa
    20701 caccctgcag gggactccgc gcctcgccgg ccgaaaaagc ccgctccgcc gcggcggctt
    20761 ccgccatggc ccgttcgggg cacagccgat gcacggcgcc cagagccggg ttcagtcgat
    20821 cgatccgctc gagcagctca cgggtcaccg caaccggcga gagctcgccg ctgcgaaatg
    20881 cccgccccag cacaccggcc gtcgggaacg ttccagagtc atctaccatg ctcgacctcc
    20941 ggcccccggg agaaggcggc attctagcag ccacctcggc ggggccccca gatgtggctg
    21001 gctcccggcg tcgcgacgtt gaataattga gccgcttagc tgatcagggg agagacacat
    21061 ggcttacacg cctgagcaac tgtcggatct cgagtgcatc cgggacgccg cgaagcgcta
    21121 ctgccgcggc gtcgaccgat tggacgaggc gttgatgaag tcggcctact ggccggaggc
    21181 cacggacgat cacggcgtgt tcgtcggcaa cgcgatggag ttcgcagaga tgtgcatggt
    21241 gagccacctg cgctggcgct ccaccagcca ctgcattttc aaccacagca tcgagctcga
    21301 ccccgacggc attcacgcgc gaggtgaggc atacaacgtc acctttctgt tccaggatgg
    21361 cgccgacgtg ctcgacacgt ggtacggccg atacctggac gtctacgaga agcgcggcga
    21421 cgagtggcgc atcttagagc gcgtctgcgt ccacgagggc accaccaccg ggccggtgag
    21481 caagatggag atcgacgccg aaaagtttcg gcacggatcc ttcgaccgac catcatccgg
    21541 gcgccgcatc ggcccctgag cacgggaaac aagcctatga acgtcgagct ggaactcctg
    21601 gcaagcggct atcgtcttct agagggtccg cgcaccgacg cagacggggc gctgtggttt
    21661 tcggacgtct acggcgggac ggtgaaccgg cgcgatccgg atggcatcat cgaggtcgtc
    21721 gtgcgcgacc ggcgaatggt cggcggcctg ctcatgcacg cgaatggcgg tttcgtgatg
    21781 accggaccca gcgtcgtcct ctggcgcgac ggtcaggagc gcgtgctgct cgagcgcgac
    21841 ggcgtgcgct tcttcaacga tctgcacgcc gacgcctcgg ggcgcatcta cgttggctcg
    21901 atacgtgcag acatcgccaa cctggacgac gagccggacc cgccggggga ggcctaccgc
    21961 atcgacctgg acggcagcgt cacggagctc tatggcggcg tgggcatctc caacggcatc
    22021 ggtttctctc ctgacggacg gactctgtac cacgtcgact cgaccagccg gggaatctgg
    22081 gcgcacgatc tggacgacgc gggcgcgttg tccaacagac gacatatcgc caagcccgcc
    22141 ttcgaaaagg gcatccccga cggcttgtgc gtggacaccg agggaaatct ctgggtcgcc
    22201 cacgttcagg gcggccgggt ggtcgttctg gaccccgggg gcaaccatgt cgacgagatc
    22261 cgcgtgcccg ccagctgggt caccagctgc gccttcggcg gctcggacta tcgggacctg
    22321 tacatcgtga gcgccgacaa cacggacgac aaagccctgg gcggctgcgt ctggcgctgc
    22381 cggcccggcg ccgcggggca tccgaccccg ctggcgcgcg tcggctgaac ggctaaagac
    22441 cgagcttgtc gcgcagcatc cgggtcaggg gctcgtggca gtccttgccg agcaggttca
    22501 tggccgtgag gcccgacagc ttcgacacca gctcaatgcc ggcggtggtg ttcgccgcgc
    22561 cgccggcaac cagatcgggc gtgcgctcga aggccgaagc aacaccgacc accgcgtagg
    22621 ggtccgatgc gcacagcagg ttgaaacgga cccggtcgcc gagcacgtcg atggccgttt
    22681 tcccgttgta gggctccagc ggcgaggcgc ccgcttcggc caccaccacg tccggcgccg
    22741 cgccagcaat ccggctgagc aaccgttcca gacgcgcggc aaaatccgcc ggatcaccca
    22801 ccgtggacgg caacccttcg tccacgaagt cgaagacggc cgctgcgccc gcatcctcgt
    22861 agctgagaac atcgcggtaa cgggccgccc cggtgagctt tgcgccaacg accctgaacc
    22921 ccaaccggct caacagcctc acgatgaggc ggccggacat ggtcttcccc gaggacatgg
    22981 aggtcccggt gatgagaacg atgggcacgg tcagggccgc gtcaggagcc ggcgtcacgg
    23041 ccccgtccat ggtctggcga acgccgttgg cgtgcacgtg ccccaggtag cgcatgccca
    23101 ttagcctcgg cagaaaagga gacacggagg tgaggcggcc gaagagcccc gcgggggtca
    23161 gcgcgtcgaa ctcatcgccc tgaatgctct gccagtcacc cacggcttcg agggtggccg
    23221 ctcgagcccc gaaagcgccg acgacccgat cgccttccat ggcttccatc aggcggccat
    23281 tgggcagctc gacgttgtga tatccggagg catcgagtac ttccccga
//
LOCUS       DBNHKV010000382         8419 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_692085, whole genome shotgun sequence.
ACCESSION   DBNHKV010000382 DBNHKV010000000
VERSION     DBNHKV010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8419)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8419
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_692085"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="AC16PP_12275"
     CDS             complement(<1..350)
                     /locus_tag="AC16PP_12275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003097238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR6482452.1"
                     /translation="MSSGTVTSGEYIQHHLTNLTFGRHPDGTWGFAQSAQEAAAMGFN
                     AINVDSMGWSIGLGLVFVAVFYTAARKATSGVPGGLQNFVEIIIEFIDEITHSIFTHK
                     NDMIAPMALTVFVWV"
     gene            complement(433..771)
                     /locus_tag="AC16PP_12280"
     CDS             complement(433..771)
                     /locus_tag="AC16PP_12280"
                     /inference="COORDINATES: protein motif:HMM:NF015834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HIR6482453.1"
                     /translation="MPYRTVALQALAGLGVVLAALAWDAREAVAALAATIVSVVPNAY
                     FAWRVSRERAAARILAAEVVKVVATVGMMGLAFATMELRPLGFFATFGVLQLAHVVGG
                     TSLAGKATSR"
     gene            complement(917..1801)
                     /locus_tag="AC16PP_12285"
     CDS             complement(917..1801)
                     /locus_tag="AC16PP_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009402535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR6482454.1"
                     /translation="MRVTKKRLGRGLDALLSGEVVADLGAGQKGPGIPEGVQVAEIGL
                     DQVVPNRYQPRRDFNEEALAELVASIRSQGVMQPVVVRRRAQGGYELVAGERRWRAAG
                     LAGLDAIPALIRDVSDEQASAMALIENIQREDLNPLEEAHALQRLRDEFGLTQQQVAD
                     AVGKSRVAVTNLLRLLNLGPAVRDLLVAGAIEMGHARALLSLDSVVQERAANDVVARG
                     LSVRQTEALVRRLQQGKGPGRPSEAVRDADTAHLERELSEKIGAPVSISCNAKGRGRL
                     VIRYGSLEELDGILGHIR"
     gene            complement(1806..2582)
                     /locus_tag="AC16PP_12290"
     CDS             complement(1806..2582)
                     /locus_tag="AC16PP_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR6482455.1"
                     /translation="MTRIIAVANQKGGVGKTTTSVNLSASLALSGSRVLLIDLDPQGN
                     ATMGSGVEKGGLERSIYHWLMEEVPLDDVLQRCASGYALLPSNTDLTAAEVELLSQPQ
                     REHRMRRRLAEDAARFDFVIIDCPPSLNILTLNALIAADGVLIPMQCEYYALEGLTAL
                     LETVEGVRQQANPSLSIEGLLRTMYDPRNALTRDVSRQLIQYFGDRVFRTVIPRNVRL
                     AEAPSHGLPAILYDRQSRGAVAYMALAGEIKRKHEEDDDP"
     gene            complement(2579..3223)
                     /gene="rsmG"
                     /locus_tag="AC16PP_12295"
     CDS             complement(2579..3223)
                     /gene="rsmG"
                     /locus_tag="AC16PP_12295"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR6482456.1"
                     /translation="MVPVAEAIAEELRGGAAELGMTLSACQVEKLVAYADLLRRWNDK
                     FNLISRRDVPRLVSRHLLDSLSISPWLGGETTLDLGSGAGLPGIPLAIAHPEREFLLA
                     DRSARKARFVEQAAMRLGLSNVSVFAGDAAALEQSFACVVARAVAGPAELWRIAGHLV
                     RPGGRLVCMSRTAGSGEGATLAADVPDAGSVLAERVAVPGNAGPHEVIVIEAAA"
     gene            complement(3207..5144)
                     /gene="mnmG"
                     /locus_tag="AC16PP_12300"
     CDS             complement(3207..5144)
                     /gene="mnmG"
                     /locus_tag="AC16PP_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR6482457.1"
                     /translation="MQFPESFDVIVIGAGHAGAEAAAAAARVGARTLLLTQSIETIGQ
                     MSCNPAIGGIGKSHLVREIDALDGIMAKAADRAGIQFRILNSSKGPAVRATRAQADRA
                     LYRQAIRELLEAQENLYLFQQSVVDLIVEDGSVRGVVTQIGLEFRAPCVVLTTGTFLG
                     GRIHIGLDNHPGGRAGDAPSNALAERLRALPFRVDRLKTGTPPRIDARSVDFSVMAEQ
                     PGDEPIPCLSFLGSPDDHPRQVSCHVTYTNDRTHSLIRAALDRSPLYTGVIEGVGPRY
                     CPSIEDKVVRFSERTEHQIFVEPEGLDTHELYPNGISTSLPFDVQVALVRSIAGFERA
                     HITRPGYAIEYDFFDPRDLHYSLETKALEGLFFAGQINGTNGYEEAAAQGLLAGTNAA
                     LKVAGRAPWCPRRDEAYLGVLVDDLVNMGTKEPYRMFTSRAEFRLRLREDNADHRLTP
                     VGRDLGLVGEKRWDAYTTKRERVERVHGRMATRNVIAGSEVADRLASLTGERMTRDQT
                     LITYLKRPGVGIAALVGLLGDVLDLQDGDLDGQVLAQVEVEAKYEGYIARQEEEIARI
                     RRHEGLKLDEGLDFAGIEGLSAELKQKLTEARPSTLARAARIPGMTAAGLSVLLVHAR
                     RARATGAVAAPEDEPARGSGG"
     gene            complement(5218..6588)
                     /gene="mnmE"
                     /locus_tag="AC16PP_12305"
     CDS             complement(5218..6588)
                     /gene="mnmE"
                     /locus_tag="AC16PP_12305"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR6482458.1"
                     /translation="MPTPPKADSLGLDVDTIAAVATPVGSGGVGIVRISGPRARAIGE
                     TVSARSLHPREAFYCRFRNAQGEVVDDGIVLYFAGPASFTGEDVVELQGHGGPVVVQM
                     VLGIVLEAGARQARPGEFSERAYLNGKMDLAQAEAIADLISSSSTAAARGAVRSLQGV
                     FSERVRKIDEAVLGVRMYVEAAIDFPEEEVDFLSEGDIAARITAILADVDDLIRDSRQ
                     GVLLRDGVTIALLGAPNVGKSSLLNALAGEDRAIVTDIPGTTRDLVRADLELAGIPVT
                     VVDTAGLRTTDDPVEREGVRRAEDQARSADLILVVTDVTDPNECSIELPEASQEIPVI
                     QVHNKIDRTSAPPGPLTGEPLPVVRVSALTGDGIDELRAQILAAVGYTEHAGTFTARQ
                     RHLLALQAAAAGLARAAGLAASADGGELVAEELRGVHTELGTLLGGITADDLLGEIFS
                     SFCIGK"
     gene            complement(6566..8296)
                     /gene="yidC"
                     /locus_tag="AC16PP_12310"
     CDS             complement(6566..8296)
                     /gene="yidC"
                     /locus_tag="AC16PP_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIR6482459.1"
                     /translation="MLPTEVLRIVLLIALGVVGYLLILQWNEDYMQRPAAPDYAAEPS
                     PSAAEPALTAAPDLPVGAELPAGTGPSDIPDESLIGQDGAETQPALSVPMPDIGERLV
                     VVRTPLLHVWIDRLGGDVVRVALPKFPVDLDRPDVPFVLLDNGLDRTYVAQSGLIGPD
                     GIDAGGDRPLYATQAQSYTLEAGELVVELSTLRDGLEVAKRYMFRSDDYLMEVSYQVR
                     NLTGRTISARMFGQLKRDRKEPYVDTSIALGPQPYLGAAFTTAENRYEKLDFDDLDEG
                     RFRTTEQGGWVAFLQHYFLSAWIANPEETNSYYGQRRPDGTYMFGFSSPVSQVASGAL
                     GEWAASLYVGPKDQKRLEEIAENLNLTVDYGFLWWLAVPLFYLLDWLHGFVGNWGVAI
                     ILLTLLVKLVLYPLSATSYKSMANMRRVAPQMKRLQERHADDRQKLSQEMMALYKKEK
                     VNPLGGCLPMLLPMPIFIALYWVLFESVELRQAPFALWINDLAAMDKYFVLPLLMGAS
                     MYLQQLLSPAVGDPMQVRMMRLMPIMFTVLFLFFPAGLVLYWLVNSVLSIAQQWYVIR
                     QTERAYAAKS"
     gene            complement(8303..8416)
                     /gene="yidD"
                     /locus_tag="AC16PP_12315"
     CDS             complement(8303..8416)
                     /gene="yidD"
                     /locus_tag="AC16PP_12315"
                     /inference="COORDINATES: protein motif:HMM:NF013932.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR6482460.1"
                     /translation="MEEFGAVRGSVLAARRLGRCHPFHPGGVDLVPTQEEG"
BASE COUNT         1548 a         2819 c         2632 g         1420 t
ORIGIN      
        1 acccagacga agacggtgag cgccatgggc gcgatcatgt cgttcttgtg ggtgaagatg
       61 ctgtgggtta tctcgtcgat gaactcgatg atgatctcga cgaagttctg cagaccgccc
      121 gggacgccgg acgtagcctt gcgcgccgcg gtgtagaaaa cggccacgaa aaccaggccg
      181 agcccgatgg accatcccat ggaatccacg ttgatggcat tgaaccccat ggccgccgcc
      241 tcctgggcgc tttgggcaaa cccccaggtg ccgtcaggat ggcgaccgaa cgtcaggttc
      301 gtcaggtgat gctggatgta ctccccggag gttacggttc cgctcgacat gctgcgttgc
      361 tctcgtcccc aatgtctgcc gaagaccacg tccccggcaa tttgaccagc gcgtcaacaa
      421 acgcgccggc gttcagcgtg aagtcgcttt tcccgccagg ctcgttccgc caaccacgtg
      481 cgccagctgc agcacgccga aggtggcgaa gaaacccaga ggccgcaact ccatcgtcgc
      541 aaaggccaat cccatcatgc ccacggtggc gaccaccttc acgacctctg ccgcgaggat
      601 cctggctgcc gcccgctcgc ggctgacgcg ccaggcgaag taagcgtttg gcacgacgct
      661 gacgatcgta gcggccagcg ccgccaccgc ttcacgggcg tcccacgcca gcgctgccag
      721 caccaccccg agcccggcga gcgcctgcag cgccacggtt cgatacggca cagacatgtc
      781 cacgtcagga cagcctgaca aacaccaacc cccgaaaggc ggcgcgtatt atagagacgg
      841 gccagagcgc gttcaattag cgtgatcctg acaaagcggc catttcgaac gacgcgcatc
      901 tgagcggcgc cggcgcctac ctgatgtgac cgagtatacc gtcgagctcc tcgagcgatc
      961 cgtaccgaat gacgaggcga cctcggccct tggcattgca ggagatcgaa accggcgcgc
     1021 cgatcttctc agagagttcg cgttccaggt gcgcggtgtc tgcatcgcgg acggcctcgg
     1081 acgggcgccc gggccccttg ccctgctgca ggcgcctcac cagcgcctcg gtctggcgaa
     1141 ccgaaagccc ccgggcgacc acgtcgttgg ccgcgcgctc ctgaacaacg ctgtccagcg
     1201 acaacagggc gcgcgcatgg cccatctcga tcgctcctgc caccagcagg tcccgcaccg
     1261 caggccccag attcagcaga cgcagcaggt tggtcaccgc aacgcgcgac ttgcctaccg
     1321 catcggcgac ctgctgctgg gtgaggccga actcgtctcg cagccgctgc agggcgtggg
     1381 cctcttccag cgggttgaga tcctcgcgct ggatgttctc gatgagcgcc atggccgaag
     1441 cctgctcatc gctaacatca cgaatcaggg ccggaatggc gtccaggccc gcaaggccgg
     1501 cagcacgcca ccgccgctca ccggcgacga gctcgtatcc gccctgggct cgtcgtctga
     1561 ccacaacggg ctgcatcact ccctgggatc tgatggaggc gaccagctcg gcaagggctt
     1621 cctcgttgaa atcccggcgg ggctggtagc ggttcggtac cacctggtcg agcccgattt
     1681 cggcgacctg aacgccttcg gggattccag ggcccttctg acccgcgccc agatcggcca
     1741 caacctcgcc cgacaacagc gcgtccagac ctctgcccag tctcttcttg gtcacgcgca
     1801 attgctcacg ggtcgtcgtc ctcttcgtgc ttgcgcttga tttcccccgc aagcgccatg
     1861 taggccaccg cgccccgcga ctgccgatcg tagagaatgg ctggaagccc gtgactgggc
     1921 gcctctgcca gccggacgtt ccgcgggatc actgttcgga acacccggtc gccgaaatac
     1981 tggatcagct gccgggaaac gtcccgcgtg agggcgttgc ggggatcata catggttcgc
     2041 aacaggccct ctatggacag cgaggggttg gcctgctgcc ggacgccttc gaccgtttcg
     2101 agcagagccg tcaggccctc gagggcgtag tactcgcact gcatgggaat gagcacgccg
     2161 tcagcggcga tcagagcgtt gagggtcagt atgttcagcg atggcgggca gtcgatgatg
     2221 acgaagtcga agcgcgccgc atcctcggcc aaccgccgcc gcatcctgtg ctcgcgctgc
     2281 ggctggctca gcagttccac ctcggcggca gtgagatccg tgttggacgg cagcaaggcg
     2341 tagccgctgg cgcatcgctg caggacgtcg tccagcggga cctcctccat caaccagtgg
     2401 taaatgctgc gctcaagccc acccttttcc acaccagagc ccatggtggc attgccctgg
     2461 ggatccaggt cgatgagaag aacccgggag ccggaaagcg caagggatgc gctcaggttc
     2521 acgctggtgg tggtcttgcc gaccccccct ttctgattcg caaccgcgat gatcctcgtc
     2581 acgcagcggc ctcgatgacg atcacctcgt ggggccctgc gttgccagga actgccaccc
     2641 gctcggcgag aacgctaccg gcgtcgggca cgtcggcggc aagggttgcg ccttccccgg
     2701 agcctgccgt ccggctcatg cagaccaacc ttccccccgg acgaaccagg tgcccggcaa
     2761 tacgccacag ctcggcaggt ccggccaccg cccgggcaac cacacaagcg aacgattgct
     2821 ccagggcggc ggcgtccccg gcaaacacgc tcacgttcga aaggcctaga cgcatcgccg
     2881 cctgctcgac aaaccgcgcc ttgcgcgccg accggtcagc cagcaggaat tcgcgttccg
     2941 gatgggcgat ggccagaggg attccaggca ggccggcccc cgagccgagg tccagagtag
     3001 tctcgccgcc cagccatggc gagatgctca gactgtcgag gaggtggcgg gataccaacc
     3061 gaggaacatc ccgccgcgag atcaggttga acttgtcatt ccatcggcgc aggagatcgg
     3121 cgtaggcaac gagcttttcc acctggcagg ccgatagggt cattcccagc tcggcggcgc
     3181 ccccccgcag ctcctcggcg atcgcctcag ccaccggaac cacgcgccgg ctcgtcttcc
     3241 ggggcggcga cagcacctgt agcccgggct cttcgggcgt gcaccagcag aacggacagg
     3301 ccggccgccg tcattccggg gatacgcgca gcgcgagcaa gcgtcgaggg tcgagcctcg
     3361 gtcagcttct gcttgagctc tgcggaaagc ccttcgatac cggcgaagtc caggccttcg
     3421 tcgagcttga ggccctcgtg ccgacggatt ctggcaatct cttcttcctg gcgcgcaatg
     3481 tagccctcgt acttagcttc cacctccacc tgcgccagca cctggccgtc gaggtcccca
     3541 tcctgcaggt ccagaacatc cccaaggaga ccgaccaacg cggcgatgcc cacgcccgga
     3601 cgcttgagat aggtgatgag cgtttgatct cgagtcatcc gctcaccggt gaggctggcg
     3661 agccggtcag ccacctccga ccccgcgatt acgttgcggg tcgccatccg cccgtggacg
     3721 cgctcgaccc gctcacgctt cgtggtgtac gcatcccagc gcttttcgcc cacgagcccc
     3781 agatcccggc ctaccggggt gaggcgatgg tccgcgttgt cttcgcgaag cctcagccgg
     3841 aactccgccc ggctggtgaa catccgatag ggctccttgg tgcccatgtt caccagatca
     3901 tccaccagaa cgcccagata ggcctcgtcg cggcggggac accagggcgc ccggccggcc
     3961 accttgagcg ccgcgttcgt tccggcgagc aggccctgag ccgccgcctc ctcgtatccg
     4021 ttggtgccgt tgatctgacc ggcgaaaaac agcccttcca gcgccttcgt ctccagcgag
     4081 taatgcaggt cccgggggtc gaaaaagtcg tactcgatgg catagcccgg ccgcgtgatg
     4141 tgggcccgct cgaatccggc gatgctgcgc accagggcga cctgcacatc gaacggcagg
     4201 ctcgtggaga tcccgttggg gtagagctcg tgagtgtcca gcccttccgg ctcgacgaat
     4261 atctgatgtt cagttcgctc cgagaaccgc acgaccttgt cctcgatact cggacagtat
     4321 cgcggcccaa ccccttcgat gaccccggtg tagagaggtg agcgatccag cgccgcgcga
     4381 ataagggagt gggtccgatc gttggtatag gtgacgtggc aactgacctg acgggggtga
     4441 tcgtcggggg agccgagaaa agacaggcag gggatcggct catccccagg ctgctcggcc
     4501 atcaccgaga aatccacgga gcgggcgtcg attcgcgggg gggtacccgt cttcagccgg
     4561 tccacgcgga acggcagcgc gcgcagacgc tccgccagcg cgttggacgg tgcatctccc
     4621 gctcggccgc cggggtgatt gtccaggccg atgtgaatac ggccgccgag aaacgtcccc
     4681 gtcgtcagca caacacaggg cgcccggaat tccaggccga tctgggtgac cacaccacgg
     4741 accgagccgt cttcgacaat cagatccacc accgactgct ggaagaggta aaggttctcc
     4801 tgggcctcta acagctcccg gatggcttgt cggtaaaggg ccctgtccgc ctgggcgcgg
     4861 gtggccctga cggctggccc cttgctggag ttgaggattc ggaactgtat gccggcgcga
     4921 tcggcggctt tggccatgat cccgtccagc gcgtcaatct cccggacgag gtgggacttg
     4981 cctattccac caatggccgg attgcacgac atctgcccga tggtttcgat gctctgggtg
     5041 agcagcagag tccgggcccc cacccgggct gccgcagcgg cagcttcggc gccggcatgg
     5101 ccggcaccaa tgacgatcac gtcgaacgac tcgggaaact gcaaagctaa tatccgccag
     5161 gttgcaagcc cgcgaagtat aagtactcgg attccgattg gaagggcgcg gccggggcta
     5221 cttgccgatg cagaagctcg agaaaatttc gccgagcagg tcgtcggccg taatgccgcc
     5281 cagcagagtg ccgagttcgg tgtgcacgcc gcgaagctct tccgccacga gctcgccccc
     5341 gtcggccgaa gcagccagac cagcggctcg cgccaagccc gcagcagcag cttgcagcgc
     5401 cagcaggtgc ctttgccggg cagtgaaggt tcccgcgtgc tcggtgtagc cgacggcagc
     5461 gagaatctgg gcgcgaagct cgtcgatacc gtctccggtg agcgcagaaa ccctgaccac
     5521 gggcaggggt tcgcccgtca atggcccagg tggcgcgctt gtccggtcaa tcttgttgtg
     5581 cacctgaatg acggggatct cctgcgacgc ttcgggcagc tcaatcgaac attcgttcgg
     5641 gtcggtaacg tcggtgacca ccaggatcag atctgccgag cgcgcctgat cctctgcccg
     5701 ccgaacgcct tcgcgttcca ccgggtcatc ggtggttcgc aaacccgccg tgtccaccac
     5761 cgtgaccggt atgcctgcga gctcgaggtc ggcgcgcacg agatcgcgcg tggtcccggg
     5821 aatgtcggtc acgatggccc ggtcctctcc ggccagcgcg ttcagcagac tcgacttacc
     5881 cacgttgggc gcgccgagaa gcgctatggt gactccgtcc cgaagcagaa cgccctgccg
     5941 ggaatcccgg atcagatcgt cgacgtcagc caggatggcc gtgatccggg cagcaatgtc
     6001 gccttcgctg agaaaatcca cttcttcctc cggaaagtcg atggccgctt ccacgtacat
     6061 ccgcacaccg agtaccgctt catctatctt ccggaccctc tcggagaaaa cgccctggag
     6121 gctgcgcacg gccccgcgag cggctgccgt ggacgagctg gaaatcagat cggcaatggc
     6181 ctcggcctga gcaagatcca tcttgccgtt cagataggcc cgttcggaga actccccggg
     6241 gcgtgcctgc cgggcccctg cctccagcac gataccaagg accatctgca cgactaccgg
     6301 cccgccgtgc ccctgcagct ccaccacgtc ctctcctgta aaggaggccg gtccggcaaa
     6361 gtagagaacg atgccgtcat cgacgacctc accctgggcg tttcggaacc tgcaatagaa
     6421 ggcctcgcgt gggtggaggc tacgggccga gaccgtctct ccgatggcgc gggctcgggg
     6481 cccggagatt cgcacgatgc ccacgccccc ggaaccgaca ggcgtcgcca cggccgcgat
     6541 cgtgtcgacg tccaggccga gagagtcagc ttttggcggc gtaggcacgc tccgtctgcc
     6601 ttatgacgta ccactgctgg gcgatggaca gcacgctgtt gacgagccag tagagcacca
     6661 gcccggccgg gaagaagaga aacagcacgg tgaacatgat gggcatgagc cgcatcatgc
     6721 ggacctgcat gggatcaccg accgcggggc tcagcagctg ctgcagatac atgctcgcgc
     6781 ccatcagaag aggcaggacg aaatacttgt ccatggccgc gaggtcgttg atccagaggg
     6841 caaagggcgc ctgtctgagc tccacactct cgaaaagcac ccagtacaga gcgatgaata
     6901 tgggcatggg caggagcatg ggaaggcatc cacccaacgg gttgaccttc tctttcttat
     6961 atagggccat catctcctgg gacagcttct gacggtcgtc cgcatgcctc tcctgcaacc
     7021 gtttcatctg aggggcaacg cgccgcatgt tcgccatgga cttgtagctc gttgccgaga
     7081 gcgggtagag caccagcttg accagcaggg tgagcagaat gatggcgact ccccagttcc
     7141 cgacgaaacc gtgcagccag tcgaggaggt agaagagagg gactgccaac caccagagaa
     7201 acccgtagtc cacggtgaga ttaagatttt cagcgatctc ttccaggcgc ttctgatcct
     7261 tggggcccac atacaggctg gccgcccact cgcccaaggc cccgctggcc acctggctaa
     7321 ccgggctgga aaatccgaac atgtaggtgc catcgggtcg gcgttgaccg tagtaactgt
     7381 tggtttcctc cgggttggct atccaggcgc tcaagaagta gtgctgcagg aaggccaccc
     7441 aaccgccctg ctcggtggtc cggaagcggc cttcgtcgag atcgtcgaaa tcgagcttct
     7501 cgtagcggtt ctctgcggtg gtgaacgcag cccccagata gggctggggt ccgagagcaa
     7561 tcgaggtatc cacgtacggc tctttgcgat cccgtttgag ctggccgaac atccgagccg
     7621 agatggtgcg cccggtcagg tttctaacct ggtaggacac ttccatgagg tagtcgtcgc
     7681 ttctgaacat gtagcgcttg gcgacctcga gaccgtcccg gagcgtcgag agctccacta
     7741 cgagctcccc cgcctccagg gtgtagctct gagcctgggt ggcatacagc ggtcgatcgc
     7801 cgcccgcatc gatgccgtcc gggccgatga gcccgctctg cgccacatag gtgcgatcga
     7861 gcccgttgtc gagcagcacg aacgggacgt cgggccgatc caggtccacg ggaaacttgg
     7921 gcaacgcgac gcgcaccacg tccccgccca gccggtcgat ccagacgtgc agcaacggcg
     7981 tcctgacgac gacgagccgt tccccgatgt ccggcatcgg cacgctgagg gccggctggg
     8041 tttcagcacc gtcttgaccg atcaacgatt cgtccgggat gtcggatggt cccgtacccg
     8101 cagggagttc cgcgccaaca ggcaaatccg gcgcggccgt cagcgcaggc tccgcagcgg
     8161 atggcgaggg ctcggccgcg tagtcgggag cagcaggacg ctgcatgtaa tcctcgttcc
     8221 actgcaggat gagcaggtaa cccaccaccc ccagcgcgat gaggagaacg atcctgagga
     8281 cttccgttgg cagcatggct gatcaaccct cctcttgggt cggaaccaga tcgacgccgc
     8341 cgggatgaaa cgggtggcag cgccccagcc ttcgcgcggc aaggacggag ccccggacgg
     8401 cgccgaactc ttcaatggc
//
LOCUS       DBNHKV010000383         3489 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1329523, whole genome shotgun sequence.
ACCESSION   DBNHKV010000383 DBNHKV010000000
VERSION     DBNHKV010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1329523"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="AC16PP_12320"
     CDS             <1..380
                     /locus_tag="AC16PP_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR6482461.1"
                     /translation="APIEVDMIVQNTGADGLTDFTFTVKRSDFEHALELLEPVRDELG
                     ARELAGDNKIAKVSVVGVGMRSHAGVAMRMFDALAEENINIQMISTSEIKISIVVAER
                     YLELAVRCIHDEFGLGEPDASAE"
     gene            506..697
                     /gene="csrA"
                     /locus_tag="AC16PP_12325"
     CDS             506..697
                     /gene="csrA"
                     /locus_tag="AC16PP_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HIR6482462.1"
                     /translation="MLILTRREGESLMIGDEISVTILSVKGNQVRLGVKAPKDVVVHR
                     EEVYQRPESRVSQGGRNNQ"
     gene            744..834
                     /locus_tag="AC16PP_12330"
     tRNA            744..834
                     /locus_tag="AC16PP_12330"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:778..780,aa:Ser,seq:gct)
     gene            924..2279
                     /locus_tag="AC16PP_12335"
     CDS             924..2279
                     /locus_tag="AC16PP_12335"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6482463.1"
                     /translation="MSSRLNVRWPMDLDGPKESLEAKQPPIDNGLDVVHPSRYTSREF
                     MEREWQHLWPRVWLLAGVVSDIPQPGDYFTYDHGHESFVVVRQQDMGIKAFYNVCPHR
                     GNRIALAEFNSTDSFTCAFHGWNFATDGKLTEITEENTFDPKLIAHRPGLNEIRCDVA
                     GGLIFINMDPQAPALRDSIGLPPGYLESYEMEKMHVVRHVRSEWRANWKTGIDAFYET
                     YHLPYVHPQTQTVMEDYSQIDLYPNGAHRMIVPFGTKAHAIADDESVDDGLRFMMIDA
                     GMSPEEFPQKASEVRGAIQAAKRKRAQRLGLDHYDNFTDGQLIDSWTTGLFPNVQLGM
                     HPEGVFIMRFMPHPTDPERFYYDNMILFRHVDDPDYTVPAWMGLPKDTDVSGAVRPEI
                     THVPVDERPDLGEVLDQDIDLVPVVQKGMRSRGFRGPLWSEMEGRLRHYHKELDRYLE
                     R"
     gene            complement(2364..>3489)
                     /locus_tag="AC16PP_12340"
     CDS             complement(2364..>3489)
                     /locus_tag="AC16PP_12340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6482464.1"
                     /translation="HRRGRRMRFGFFAMPEHFPWENWTLAYDRDIDRMVRAEQLGFDE
                     FWIGEHHSGGYEPVPVPELMIAKASALTARIKLGTGVVNLPYHDPFLVAERLAFLDHL
                     THGRLLYGVGGGGLPSDRALFGLPAEEARPRMLEAIDVIERVLAAREPISHEGTFWRF
                     ENRRLQVPPFQAQPKLAIAGLTGTHNFTTCGQRGYAALSVYFTPVLIEANPGVPDLVD
                     HGRALAQAAEEAGRDPDEARDDWRISREVYVAESRSQALDDIRDGVRASYDYLMRLGL
                     GPLMKRDAAMPDADVTFEWMVDNIPWIIGSPEECVDQIGSLYEAVGGFGTLLINCRDW
                     VDDAQFHRSMELFARQVVPAFREREHQDLRRSLARDALGE"
BASE COUNT          705 a         1059 c         1098 g          627 t
ORIGIN      
        1 gtgcccccat cgaggtcgac atgatcgttc agaacacggg cgctgatggc ctcaccgact
       61 tcacgttcac cgtcaagcgg tccgacttcg agcacgctct ggagcttctg gagccggtgc
      121 gcgacgagct cggcgcgcgg gaacttgccg gtgacaacaa gatcgccaag gtgtccgtgg
      181 tgggtgtcgg catgcgctcc catgccggcg tcgccatgcg gatgttcgat gctctggccg
      241 aggagaacat aaacatccag atgatatcga cttccgagat aaagatttcc attgtcgtag
      301 cggagcggta tcttgagctt gccgttcgct gcattcatga tgagttcgga ctgggcgagc
      361 ctgacgcgag cgccgaatag ggcgtcgaat cgcgaaacga taaggcgaaa cggttagcac
      421 ggctcgatgg agcgggcccg atcagcctca attgataagc cgcgagcgag ggaaaggaca
      481 gcacgccggg cagcggagca caggcatgct gatactgacc cgacgggaag gtgaaagcct
      541 gatgatcggc gatgagattt cggtgacgat tctcagcgtc aaaggtaatc aggtccgtct
      601 cggcgtcaaa gcgccgaaag acgtggtggt tcaccgcgaa gaagtctacc agcggccgga
      661 atcacgcgtg tcccaaggcg ggcgcaacaa ccagtagcat cgctggctac ggtggccgtc
      721 ggccggtaaa cttcggccct cccggagagg tggccgagtg gctgaaggcg ctcccctgct
      781 aagggagtat gggttaatag ctcatcgagg gttcgaatcc ctccctctcc gccagaacta
      841 caaccgcggt cgctcccggt gtcttggaga caacggctcc gtgctaggat agtggtccga
      901 ttccacggtt gaggaggaaa cccatgagct cgcgcctgaa cgtccggtgg ccgatggatc
      961 tcgatgggcc aaaagagtcg ctcgaagcca aacagccgcc gatcgacaac gggctggatg
     1021 tggtccatcc atcccgctac acatcccggg agttcatgga gcgcgagtgg cagcacctgt
     1081 ggccgcgggt gtggctgctt gccggcgtgg tgtccgacat accccagccc ggtgactact
     1141 tcacctacga ccacggtcac gagtcgttcg ttgtggtgcg ccagcaggac atgggtatca
     1201 aggccttcta caacgtctgc ccacaccggg gcaatcgaat tgcgcttgcg gagttcaaca
     1261 gcaccgacag cttcacgtgc gcttttcatg gctggaattt tgcgactgac ggcaagctca
     1321 ctgaaatcac agaggagaac acgttcgatc ccaagctcat tgctcatcgg cccgggctca
     1381 acgagatccg ttgtgacgta gccggcggct tgatcttcat caacatggat ccgcaggccc
     1441 cggcgctgag agactcgatc ggcttgcccc cgggctatct cgaaagctat gagatggaaa
     1501 aaatgcacgt ggtccgccat gtgcgcagcg aatggcgggc aaactggaag accggaatcg
     1561 acgcgttcta cgagacttac cacctgccct acgtgcaccc gcagacgcaa acggtcatgg
     1621 aagactacag ccagatcgac ctgtacccga acggcgctca ccgaatgatc gtccccttcg
     1681 gcaccaaggc gcacgcgatc gccgacgatg agagtgtcga cgacgggctg cgattcatga
     1741 tgatcgatgc cggtatgtcg cctgaagagt ttccgcagaa ggcgagcgaa gtgcgcggcg
     1801 ccattcaggc agcgaagcga aagcgcgccc agcgcctggg gcttgatcac tatgacaatt
     1861 ttaccgatgg acagctcatc gacagttgga cgacgggcct gttcccgaat gtccagcttg
     1921 gcatgcatcc cgaaggtgtc ttcataatgc ggttcatgcc gcatccgacc gacccggagc
     1981 gcttctatta cgacaatatg atcttgtttc gccacgtgga cgatcccgac tacacggtgc
     2041 cagcgtggat ggggctacca aaggacaccg acgtgtcggg tgcggtgcga ccggagatca
     2101 cacacgtgcc ggtggacgag cggcccgatc ttggggaagt gctcgaccag gatatcgatc
     2161 tagtgccggt agtccaaaag ggcatgcgct cccgggggtt ccggggaccg ctttggtcgg
     2221 agatggaagg gcgcctgcgg cactaccaca aggagctgga ccgctacctg gagcgctagg
     2281 ctgcgcccgc cgttgccagc ttgcggtcgg ggggctggtt aagcttccga ccgcagccac
     2341 cgccagccaa ctttctgatg cgcttactcg ccgagggcgt cccgcgcgag ggagcgacga
     2401 agatcctgat gctcgcgctc tcgaaaagct ggaacgacct gccgagcgaa cagctccatg
     2461 gagcgatgga actgggcatc gtccacccag tcccggcagt tgatcagcag agtgccgaac
     2521 cctccgaccg cctcgtagag cgaaccgatc tgatctacgc actcctcggg cgagccgatg
     2581 atccagggaa tgttgtccac catccactcg aaggttacgt ccgcatcggg catggccgcg
     2641 tcccgcttca tcagcgggcc cagccccagc ctcatcagat agtcgtaaga ggcgcgcact
     2701 ccgtcgcgga tgtcgtccag agcctggctg cggctctcag cgacgtagac ctcccggctg
     2761 atgcgccagt catccctggc ttcgtccgga tcacgcccgg cctcctcagc cgcttgcgcc
     2821 agtgccctgc cgtggtccac cagatccggg acgcccggat tggcttcgat gaggaccgga
     2881 gtgaagtaaa cgctgagcgc tgcgtagccg cgttggccgc aggtagtgaa gttgtgggta
     2941 cccgtcaacc cggcgatggc cagcttgggt tgggcctgaa agggaggtac ctgcagtcgc
     3001 cggttctcaa atcgccagaa cgtcccctcg tgggagatgg gttcccgcgc ggccaggacg
     3061 cgctcgatga cgtcgatggc ttccagcata cgcgggcgcg cctcctcggc cggcaggccg
     3121 aacagagccc ggtccgacgg cagaccgccg ccgccgacgc cgtaaagcaa ccgcccgtgg
     3181 gtcaggtgat ccagaaaggc caggcgctcg gcgaccagaa aaggatcgtg gtagggcagg
     3241 ttgacgacgc cggtccccaa tttgattcgg gcggtcaggg cagaagcctt ggcgatcatc
     3301 agttcaggca ccggcacggg ctcgtacccg ccgctgtggt gctcgccgat ccagaactcg
     3361 tcgaaaccga gttgctctgc cctcaccatt cggtcgatgt cgcgatcgta ggcgagggtc
     3421 cagttctccc aggggaaatg ttcgggcatt gcgaaaaagc caaacctcat tcgcctacct
     3481 cgtcggtgc
//
LOCUS       DBNHKV010000384         3224 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_976892, whole genome shotgun sequence.
ACCESSION   DBNHKV010000384 DBNHKV010000000
VERSION     DBNHKV010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_976892"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="AC16PP_12345"
     CDS             <1..510
                     /locus_tag="AC16PP_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482465.1"
                     /translation="GLGLAFVNATSQHLWASVERLRDELAIGRDYFQVFQGAVVLNEQ
                     DEVLLVQQHGKWGFPGEYLVVGEDAETALKAMLTRRFALADLTIDGVLKVGSGATIDL
                     PEAAVFRVYHRVTTSTAEISASAEEAYRKVRWVENRRSVAQMAFVSKELRELANAALL
                     WAAGRRRAG"
     gene            complement(536..2902)
                     /locus_tag="AC16PP_12350"
     CDS             complement(536..2902)
                     /locus_tag="AC16PP_12350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HIR6482466.1"
                     /translation="MLTENRRVWQVLAEKLFWHNGNYAPDGNLAHMRAWLVGLVLGHL
                     VLAAPVAPLPLPVPTAWLMLAGILLGVVRRGLPACGFLVGFAWAAFAMQQLLESRLPG
                     CLDGAEELMVVDVVSEPRRREDGAYTFSADVRSTRAGLCADVLGRRLRLTWYEAPGIG
                     QGERWRVLARLRTPWAYQNPGGFDFERWLLAEGLNGSGYVVSGTRIGGPRASWRAPWL
                     EGVRGYLETEHPAAAGVLYALVSGDGSLIASAGWRTLRDTGTVHLMVVSGLHVGLVAG
                     MGYQIARLAVRALPLVLLWVPARRAGALGGVCAASGYVALTGGGVPGLRALCMSALVL
                     GALAWGRRLSPWLVWLVALALVLVGQPAVVHRQGLWLSFGAVAVLIWFFAGRSVREPR
                     LAGVAAALVRAQYALLLGLSPLLVMLQGQIPSSGPVANLVAVPVISFLVIPAALIGAL
                     LMELVPFLGEAALGMAAVVVAQLMGVLEASASLPPLVAAPRLLPVFVALATGLVMLAC
                     GGSGRHLLLCLAWAVWALPPDHGLPRGEFRVLAMDVGQGSAVLVDTKRHRLLFDAGPR
                     YPSGFDLGAQVVLPTLVATGSPRLDALVISHADIDHSGGKGAVVGAVPVGEVYESRPD
                     SESSRCSAGQSWRWDGVDFRFLHPPPLARARGLGSNDASCVLLIDNGRERALLPGDIG
                     AAVEGQLLRALDPVDVLLAAHHGSASSSSRAFVRVLEPDVVFISAGRRNRYGHPHPDV
                     VGRFAEVGARVHSTAEHGALVWDSRTPGSVRRWRRDTPRYWVQGSQAD"
     gene            2918..>3224
                     /locus_tag="AC16PP_12355"
     CDS             2918..>3224
                     /locus_tag="AC16PP_12355"
                     /inference="COORDINATES: protein motif:HMM:NF021363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HIR6482467.1"
                     /translation="MRKWLKEPEIWHLHRRSVAGAAFIGMFCAFLPVPFQMLIAAVLA
                     VASRCNLPMSVVLVWITNPLTIPPMFYFAYRLGAWLLNMEIEVATIELSWEWLKTHIG
                     "
BASE COUNT          623 a         1121 c         1045 g          435 t
ORIGIN      
        1 ggtctgggcc ttgccttcgt caatgccacc agtcagcacc tgtgggctag cgtagagcgt
       61 ctgcgtgacg agctggcgat cggccgggac tacttccagg tgttccaggg cgcggtcgta
      121 ctcaacgagc aggatgaggt gttgctcgta cagcagcacg gaaagtgggg gtttcccggc
      181 gagtacctgg tcgtcggcga ggacgcggaa acggcgctga aggcgatgtt gacccgccgt
      241 ttcgctctgg ctgacctgac gatcgacggg gtcctcaagg ttggcagcgg ggctacgatc
      301 gacctgcccg aggcagccgt cttcagggtc tatcaccggg tgaccacctc gacggcggag
      361 atctcagcca gcgcagagga agcgtatcgc aaggtccgct gggtggaaaa ccgacgcagc
      421 gttgcccaga tggcattcgt cagcaaagag ctgcgcgagc tcgccaacgc cgcgttgctc
      481 tgggcagccg ggcgccggcg cgccggctga acaggccaag ggggcgaccg gcgcgtcagt
      541 ccgcttgcga tccctgcacc cagtagcgcg gcgtatcccg ccgccaccgc cgcaccgagc
      601 caggcgtccg gctgtcccat accaacgccc cgtgctccgc ggttgagtgg acgcgcgcgc
      661 cgacctccgc aaagcgtccg accacatcgg gatgaggatg gccgtagcgg ttacgtcggc
      721 cggcgctgat gaagaccacg tccggctcca gcacgcgcac gaaagccctg gacgacgagg
      781 aagcgcttcc gtggtgggcg gccagaagga cgtccaccgg atccagtgct cgcagcagtt
      841 gcccttctac ggcggcaccg atgtctccag gcagcagcgc gcgttcccgg ccgttgtcga
      901 tgagcagcac gcaggatgcg tcattgctcc ccaggccgcg agcgcgtgcc agcggcggcg
      961 ggtgcaggaa tcggaaatca acgccgtccc aacgccagga ctggccagcc gagcagcgtg
     1021 aggattctga gtcgggccgg gattcgtata cctcacctac gggcacggcg cccaccacgg
     1081 cgcccttgcc gccgctgtga tcgatatccg catgactgat caccagggcg tccagccgcg
     1141 gggagccggt ggcaacgagg gtgggtagga cgacctgggc ccccaggtcg aagccgctgg
     1201 gatagcgcgg gcccgcatcg aacagcagcc gatgcctctt cgtgtccacc agcactgcgc
     1261 tgccctgccc cacgtccatc gcgaggactc tgaactcacc cctgggcaag ccatgatcag
     1321 ggggtagcgc ccagaccgcc caggcgaggc acaacaaaag gtgcctgccc gaaccgccac
     1381 acgccagcat gacgagcccg gtagccaggg ccacgaacac cggcaacagt cgtggagcgg
     1441 ccacgagcgg gggcagcgac gccgacgctt cgagaacgcc catcagctgc gcgacgacca
     1501 cggcagccat cccgagcgcg gcctcgccga ggaacgggac cagctccatc agcagcgcgc
     1561 cgatcagcgc cgccggaatg accagaaagc tgatgacggg caccgcgacg aggttggcca
     1621 ccggtcccga tgagggaatc tgtccctgga gcatgaccag cagcgggctg agccccagca
     1681 gcaacgcgta ttgagcgcga acgagagctg cagccacccc agcgagcctc ggttcccgga
     1741 cgctgcggcc tgcgaagaac cagatcagca ccgccaccgc gccgaaggag agccagagtc
     1801 cctgccgatg gactaccgcc ggttggccga ccagcaccag cgccagggca accagccata
     1861 ccagccaggg cgacagccgc ctgccccacg ccagggcccc cagaaccagc gcggacatgc
     1921 acagtgcccg caatccggga accccaccgc cggtcaatgc aacgtatccg gatgcggcgc
     1981 aaacaccgcc aagggctccc gcccggcgag ccggaaccca caggaggacc agcgggagtg
     2041 cacgaacggc taaacgcgca atctgatagc ccatgccggc cacgagtcca acgtggagcc
     2101 cggatacgac catcagatga accgtgcccg tatctcgcag ggtgcgccag ccggccgagg
     2161 cgatgagcga gccgtctccg cttaccaggg cgtagagcac accggcggcc gccggatgtt
     2221 ccgtttcgag gtaacctcgc acgccttcga gccacggggc acgccaggat gcgcgcgggc
     2281 cgccgatccg cgtgcccgat acgacgtagc cgctcccgtt cagcccttcg gcaagcagcc
     2341 agcgttcgaa gtcgaaccct ccgggattct gatacgccca tggcgtgcgc agccgcgcca
     2401 gaacccgcca gcgctcgcct tgcccgatcc cgggagcttc gtaccaggtc agccgaagcc
     2461 gacggccgag gacgtcggcg cagaggccgg ccctggtgct gcggacgtcg gccgaaaaag
     2521 tataggcccc gtcctcccgc cggcgcggct ccgagacgac atcgacgacc atgagctcct
     2581 cggctccgtc caggcagccg ggcaggcggg actccagcag ctgctgcatc gcaaacgccg
     2641 cccaggcgaa ccccaccaga aacccgcagg cgggaagccc gcgccggacg acgcccagca
     2701 agattccggc cagcatgagc cacgccgtcg ggacaggcag tggaagcggg gcgacgggcg
     2761 cggcgagaac gaggtgaccc agaacgagcc caacaagcca agcccgcatg tgtgcaaggt
     2821 ttccatcggg tgcataattt ccgttatgcc aaaaaagttt ctccgcaagc acctgccaaa
     2881 cccgacggtt ctcagtgagc atcgggcgct gcgccccgtg cgaaaatggc tcaaggaacc
     2941 ggaaatctgg cacctgcacc gccgttcggt tgccggcgcg gctttcatcg gcatgttctg
     3001 cgctttcctg ccagtgccgt ttcagatgct catcgcggcc gtgcttgccg tggcgtcacg
     3061 ttgcaacctg cccatgagcg tcgtcctggt ctggataacg aacccgctga ccatcccacc
     3121 catgttctac ttcgcctatc ggctgggcgc atggctgctg aacatggaaa tcgaggtggc
     3181 cacgatcgag ctcagctggg aatggctcaa aacccacatc ggac
//
LOCUS       DBNHKV010000385         4855 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_834600, whole genome shotgun sequence.
ACCESSION   DBNHKV010000385 DBNHKV010000000
VERSION     DBNHKV010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_834600"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="AC16PP_12360"
     CDS             complement(<1..1169)
                     /locus_tag="AC16PP_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6482468.1"
                     /translation="MSEDFGEIRPKSVPLTHSAPERPSASTRPSLRPLAAGASVLLVM
                     ATAFVVFVVVPDLVQPPTLPGTPATDTAGATSDSPRPAARDEKLPPFQALMKEQARAR
                     AQEELARFVELQLDLEQTMQVGQWGQAEYDAAKRLATAGDEQFLGESFEAAIESYAKA
                     ATALAALIEDGKARFASAMRDGRTALERRDEKNAAGAFARALTISPDDQEAQQGARRA
                     ALLPEVNTAMREAKNHELAAEYRDALAVYERVRAMDPKTAGLEAGIRAARAGIQAETV
                     KGHLSSGFAALEAGRLDAARAAFGQALKLDPGNPVALGGLEQVDDASAGRRLATLRGR
                     AEAAEAAEDWETALQSYVAVLESDGNIQFARDGRQRALAQRRAATGLSRIAASPE"
     gene            complement(1169..2365)
                     /locus_tag="AC16PP_12365"
     CDS             complement(1169..2365)
                     /locus_tag="AC16PP_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482469.1"
                     /translation="MKLLAVALLAGLCAGCVQEVVRTVDMTPPEQLDRVQTEDELLDI
                     GVAILDPNVPESYDEQIKQLVQPEIRRAEANFIPYVAKNLLQSTGNWGAVRVVPRPTH
                     AVDVVVEGKILHSTGENLAVEMSVHDATGRHWFSRRYEALASKYAYEEAIPQSIDPFQ
                     AIYKQLADDMLDYRKGLSYDEVMVIRRTAEMKFAQDFAPDAFADHITTTSEGEFELKR
                     LPAENDPMLSRVQRVREREYLFIDTLDEYYESFHRDMFESYQSWRRATYDEAIAYKAL
                     RAQSRARTIGGATAIIAGVAAMSESSDPYVDAGGIVSVIGGAMTIKGAIEKRAEAQIH
                     AEVLQEVGVAAEAEIVPYTIELENQTVRLQGSVDEQYEELRRILRRIYFEDLGLPAPE
                     EQPEET"
     gene            complement(2411..3109)
                     /locus_tag="AC16PP_12370"
     CDS             complement(2411..3109)
                     /locus_tag="AC16PP_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482470.1"
                     /translation="MPGSPEGPDSGEPGEAGPGEKEPGVAGTEEAGSGQEQPGASGAG
                     DPGEDSPGWVDGDEAGEGGWETSNQVPAQTAEMPPMPSGSGGGPAEQAEPGQSEGDGE
                     LDKALESFDGEIMSEREVIMARSNENAGKTGSSIPMPGSGSGSGSQTGGSEGVPGAEQ
                     GGSDVPGGPAGMPRASSAPPPPMASAGDIPDDIPDAKDDDIIARQLREAAMKETDPEL
                     KEKLWDEYRRYKGA"
     gene            3225..3431
                     /locus_tag="AC16PP_12375"
     CDS             3225..3431
                     /locus_tag="AC16PP_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482471.1"
                     /translation="MLLPLVGGAPPGWLGEDWGLCLPGFDGELGWLGELLGWLGELLG
                     EEGGVDGLGMLGGFGGWGGVWLLD"
     gene            complement(3579..>4855)
                     /locus_tag="AC16PP_12380"
     CDS             complement(3579..>4855)
                     /locus_tag="AC16PP_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6482472.1"
                     /translation="DAHLMLLTRLTTGLLVALTMVGAHSAERIDEMLDVDDPALADVE
                     SRIRAREFDQPITWLETHIDTLESISHRYDPELVRPLVLLGDAELGKGNYGTALEHYG
                     RAVHVSRVNDGLHSASHVEIVYREAQAYKAMGNYEQANNREEYAYQVLKRAHGGMDED
                     LLPGVYKLARWYRDTQNLFSARAMYQHALEILIANGKHDDLESIPAYEGIAWSYRLER
                     FPPYFMSGGETRASEFSSAYEESISINNFPAGERALQQIIRIDRNTDSINPVVVSEAI
                     LELADWYLLFDKTKRAYPLYEYVYDALEELEPGLAGTYFGEPKLLYFPVPQDPRPPDL
                     QERGDARTGYVELAYEISDSGYPRSLKTVASEPPGLMDFRVRKSLRLSRYRPMIVEGQ
                     ALATQDFTYRYEFDYFEELEPAQEVVEEEQDE"
BASE COUNT          717 a         1576 c         1605 g          957 t
ORIGIN      
        1 ttctccgggc tggccgctat ccgcgagagg ccggttgccg cgcgacgctg cgcgagcgcg
       61 cgttgacgcc cgtcgcgggc aaactgaatg ttgccgtctg actcgagcac ggcgacgtag
      121 ctctgcaacg ccgtctccca gtcctcggcc gcctcggccg cttcggccct gccgcgcagc
      181 gtcgccagac gccggccggc agaggcgtcg tcgacctgtt cgaggcctcc gagcgccacc
      241 gggttgccgg gatccagctt cagggcctgg ccaaaggcag cgcgcgcggc atcgagcctg
      301 ccggcctcca gcgccgcgaa accggaggag agatggccct tgaccgtctc cgcctggatg
      361 ccggcgcggg cagcccggat gcccgcttcg aggcccgcgg tcttcgggtc cattgcgcgc
      421 actcgctcgt agacggcgag ggcgtcccga tactccgcgg ccagctcgtg attcttggct
      481 tcccgcatcg ccgtgttcac ctcgggtagc agcgcggctc gccgcgcgcc ttgctgagct
      541 tcctgatcgt cgggagagat ggtcaacgcc cgggcgaacg cccctgcagc atttttctcg
      601 tcgcgtcgtt ccagggccgt gcgaccgtcc cgcatcgcgc ttgcgaaacg cgccttgccg
      661 tcctcgatga gggcggccag cgcagttgcc gccttcgcgt agctttcgat ggcggcctcg
      721 aacgactccc cgagaaactg ctcgtcgccg gcggtggcga ggcgcttggc ggcatcgtat
      781 tcggcctgtc cccattgccc gacctgcatg gtctgctcca ggtcaagctg gagctccacg
      841 aaccgtgcca gctcctcctg agccctggcc cgcgcctgct ccttcatcag cgcctgaaat
      901 ggaggcagct tctcgtccct ggccgccggg cgtggcgagt ccgaggtcgc gccggcggta
      961 tcggtggccg gcgtgccggg gagcgtcggg ggttggacca gatcggggac gaccacgaag
     1021 acgacgaagg ctgtcgccat gacgagcagc acggaggcgc ccgctgccag gggcctgagc
     1081 gaggggcgcg tggaggccga cggccgttcc ggagcgctgt gcgtgagcgg gaccgattta
     1141 ggccggatct cgccgaaatc ttcgctcatc aggtctcttc tggttgttct tcgggagcag
     1201 gtagacccag atcctcaaag taaatgcggc ggagaatcct acgcagctct tcgtactgct
     1261 cgtccaccga cccctggagc ctcacggtct ggttctccag ctcgatggtg taggggacga
     1321 tctcagcttc cgcggcgaca ccgacttcct gcagcacctc cgcgtgaatc tgcgcctcgg
     1381 cgcgtttctc gatggcacct ttgatggtca tggcgccgcc aatgacgctc acgatgccgc
     1441 cggcgtccac gtatgggtcg ctgctttcgc tcatggcggc gaccccggca atgatggccg
     1501 tggcgccgcc gatggtccga gcgcgcgact gcgccctcag cgccttgtag gcgatggctt
     1561 cgtcgtaggt cgctcggcgc cagctctggt aggactcgaa catgtcgcgg tggaaactct
     1621 cgtagtactc gtccagcgtg tctatgaaga ggtactcgcg ctcccgaacg cgttggacgc
     1681 ggctcagcat ggggtcgttc tcagcgggaa gacgtttgag ctcgaactct ccctcggagg
     1741 tggtggtgat gtggtcggcg aacgcgtccg gcgcgaaatc ctgcgcgaac ttcatctccg
     1801 cggtacggcg gatcaccatg acctcgtcgt agctcaggcc cttgcgatag tcgagcatgt
     1861 cgtcggccag ctgcttgtag atggcttgaa acgggtctat ggactgcgga atcgcctctt
     1921 cgtaggcgta tttgctggcc agcgcttcgt agcgccggga aaaccagtgg cggccggtcg
     1981 catcgtgcac cgacatctcc acggccaggt tctcgcctgt ggaatggaga atcttgccct
     2041 caacgacgac gtcgacggcg tgggtcgggc gcggcacgac gcgtacggcc ccccagtttc
     2101 ccgtgctctg cagcaggttc tttgcgacgt agggaatgaa gttggcctct gcgcggcgga
     2161 tctccggctg aacgagctgc ttgatctgct cgtcgtagct ctccggcacg ttcgggtcga
     2221 gaatggccac gccgatgtcg agcagctcgt cctcggtctg aacgcgatcg agctgctcgg
     2281 gaggggtcat gtccacggtt cggacgacct cctgaacgca gccggcgcag agaccggcga
     2341 gcaacgccac tgcgagtaac ttcaatcttt ctttcgagtc tggctgcaca cgcatttgcc
     2401 tactgctggt tcaagcgccc ttgtagcgcc gatactcgtc ccatagtttt tccttcagct
     2461 ccgggtccgt ctccttcatc gcggcctctc taagctggcg tgcaatgatg tcgtcgtcct
     2521 tcgcgtcggg gatgtcgtcg gggatgtcgc ctgccgaggc catgggaggc ggcggtgcag
     2581 agctcgccct gggcataccc gcggggccgc ccgggacgtc ggagcctccc tgctccgcgc
     2641 cgggaacgcc ttccgacccg ccggtctggc ttccgctgcc gctcccggaa cccggcatgg
     2701 gaatcgacga gccggttttt ccggcgtttt cgttggagcg tgccatgatg acctcccgct
     2761 ccgacatgat ctcgccgtcg aaactctcca gcgccttgtc gagctctccg tcaccctcgg
     2821 attggcccgg ttcggcctgc tctgcggggc cgcctccgga acctgacggc atgggcggca
     2881 tctcggccgt ctgcgctggc acctggttgc tggtctccca acccccttca cccgcctcgt
     2941 cgccatccac ccaaccgggc gaatcctctc cgggatcgcc ggcaccggat gcgccaggct
     3001 gctcctgtcc gctacctgct tcctcggtgc ccgcgacgcc cggctccttt tcgccgggtc
     3061 cggcttcccc aggctcaccg gaatccgggc cttctgggga tcccggcatt cccggcattt
     3121 cgggcagctg ctcggtgccg gtatcttcgc cggtgcccgc agtgctgtcg ggaggcggag
     3181 gaatgtctgc ctcgccggct ctgggctgac ctcccggggg cggggtgctg ctgccgctgg
     3241 tcggcggagc gccgcctggc tggctgggag aggactgggg gctctgtttg ccgggtttcg
     3301 atggggaact cggctggctc ggcgagctgc tgggctggct tggcgagctg cttggcgaag
     3361 agggtggtgt agacgggctc ggcatgctgg gtggctttgg gggctggggc ggcgtctggc
     3421 tgctggactg acagcccgac aaggcaagca cgacggccag cgcgagggtg cgggccgaac
     3481 gccgttgcga tctggacatg ctcacctttc cgacatcccg ctgcgcagac gcggggagcc
     3541 taccgcgagg aagctgaacg gtggctgtac gagccggttt actcatcctg ctcctcctca
     3601 acgacctcct gggcaggctc gagctcctcg aaatagtcga actcgtagcg atacgtgaag
     3661 tcctgggtcg ccagagcctg tccctccacg atcatcggcc ggtatctgga caagcgcagg
     3721 ctcttacgga cgcggaaatc catcaggccc gggggctcgg atgccaccgt cttgagcgag
     3781 cgggggtagc cgctgtccga gatttcatag gctaactcga cgtagccggt ccgggcgtcg
     3841 ccgcgctcct gcagatcggg cggccgggga tcctgcggta ccgggaaata gagcagcttg
     3901 ggctcgccga aataggtccc cgccaaaccg ggctcgagct cctccagagc atcgtaaacg
     3961 tactcgtaga gtggataagc gcgtttggtc ttgtcgaaga gcagatacca gtcggcgagt
     4021 tcgagaatgg cctcactgac cacgacgggg ttaatgctgt cggtgttccg gtcaatccgg
     4081 attatctgct gcagcgcccg ttcgccggca gggaaattgt tgatggagat cgactcctcg
     4141 taggcagagg agaactcgct ggccctggtt tcaccgccgg acatgaagta gggcggaaaa
     4201 cgttcgagtc gatatgacca cgcgatgccc tcataggcgg gtatagactc gaggtcgtcg
     4261 tgttttccat tcgcgatcag gatctccagg gcgtgctgat acatggcgcg agcggagaag
     4321 aggttctgcg tgtcccgata ccaccgtgcc agcttgtaca cgccgggcag cagatcctcg
     4381 tccattccac cgtgggcccg cttgagaacc tgataggcgt attcctcgcg gttgttggcc
     4441 tgctcgtagt tgcccatggc cttgtaagcc tgggcttccc gatacacgat ctccacgtga
     4501 gaggcgctgt gcaggccgtc gttgacgcgg ctgacgtgga cggcccgccc gtagtgctcc
     4561 agcgccgtgc cgtagttgcc tttacccagc tctgcgtcac ccagcagaac cagcggccgg
     4621 accagctcgg ggtcgtagcg gtgactgata ctttcaagcg tgtcgatgtg ggtttccagc
     4681 cacgtgatgg gctggtcgaa ctcgcgtgcc ctgatccgcg attcgacgtc cgcaagggca
     4741 gggtcgtcta catcgagcat ctcgtcgatg cgctcggcag agtgcgcgcc gaccatggtc
     4801 agcgctacca gcaatcctgt tgtcaaccta gtcaagagca tgaggtgggc gtcag
//
LOCUS       DBNHKV010000386         2644 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_195675, whole genome shotgun sequence.
ACCESSION   DBNHKV010000386 DBNHKV010000000
VERSION     DBNHKV010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_195675"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1866
                     /locus_tag="AC16PP_12385"
     CDS             <1..1866
                     /locus_tag="AC16PP_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6482473.1"
                     /translation="NLASGGGRARFVQIRGIGERGQFAEPLNSSVGLLLDGVDMSGVG
                     TVATLFDVEQVEILRGPQGTLYGANALAGLINVVSADPSDELYTRLSLDAGNYGAFGV
                     GGVISGPLGDNVGYRLAVQSYGDDGFMDNRFLDRDDTDDHDELTIRGKLHWQVGPETD
                     LRLTLGHVDVDNGYDAFSLDNDRNTLSDAPGQDRQQSTFGSAQLNTALGEAVTLQATV
                     GYADSDIDYGYDEDWTYAGFDPIGYTSEDRYRRDRQTLNFDVRLLSGQAARMPGSVDW
                     VLGVYGLHQEVDLERDYTFFFGEPFASRFEVDRLAVYGELGVQMSQRIRASLGLRFEH
                     HESDYSDSEAVAADPKDDMVGGRLVLEADVADDAMVYFSASRGYKAGGFNTSGTLERE
                     LRDFDPEKLWNYEVGVKGTSADGRLQARVAAFLMKRDDVQVSTFIAVPREDGSTEFID
                     LIDNAAEGTNAGLEAEVLWMPTEQLEVFASFGWLDTEFDDFINAAGEDLDGEEQAHAP
                     GYQFHVWGEYRFADGWFLRLAVEGRDAFYFTDGRRFAPNAEDLKSDSYELVHASAGYE
                     ADRWRVSAWGRNLTDEDYAVRGFYFGNDPRDFYTARGFTQLGEPRRYGITLQLEL"
     gene            complement(1893..>2644)
                     /locus_tag="AC16PP_12390"
     CDS             complement(1893..>2644)
                     /locus_tag="AC16PP_12390"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HIR6482474.1"
                     /translation="STPASLWVNSGANDRGGPMSSRKKSDKKVTRVRMLIVGFTILVG
                     IVVLGYGTLYSTGLGEGEFLAGEHYRVIENPPRRRAGAPIMVREYFSYGCIHCRNFEP
                     LLDEWRATQPERTAFERSPVAFSPAWQLLAQTYFTLKRLDVLEQNHMRLFRAIHDNAR
                     QFATPESMADFVDGNGVTRAEFLRMFNSPEVRGDLREADAIQRNIVISSVPTLVVDDR
                     YVVNMDLGRKRALDVVDYLISQQLAETAAAP"
BASE COUNT          459 a          836 c          878 g          471 t
ORIGIN      
        1 aatctggcca gcggcggcgg ccgggcccga ttcgtgcaga tccgcggcat cggtgagagg
       61 ggccagttcg ccgagccgct caactcgtcg gtagggcttc tgctcgacgg cgtagacatg
      121 agcggggtcg gcaccgtcgc cacgctcttc gatgtggaac aggtggaaat tctgcgcggg
      181 ccccagggga cgctctacgg ggcgaatgcc ctggctggcc tcatcaacgt ggtcagcgcc
      241 gaccccagcg acgagctcta cacgcggctc tccctggatg ccggcaacta cggggcgttc
      301 ggggtgggtg gcgtgatctc cggtcccctg ggggacaacg tcggctatcg cctggccgtg
      361 cagagctacg gtgacgatgg cttcatggac aaccgctttc tggatcggga cgacacggat
      421 gaccacgacg agctgaccat ccgcggcaag ctccattggc aggttggccc ggaaaccgat
      481 ctgcgactca cgctgggcca cgtggacgtg gacaacggct acgatgcgtt ctcgctcgac
      541 aacgaccgca acacgctttc cgatgccccg ggccaggacc gccagcagag cacgttcggc
      601 agcgctcagc tgaacaccgc gctgggcgag gccgtcaccc tacaggctac cgtgggctat
      661 gcggacagcg acatcgacta cggttacgac gaggactgga cctacgcggg tttcgacccc
      721 atcggctaca cctccgaaga ccgctaccgg cgcgatcgcc agacgctcaa cttcgatgtc
      781 cggctgcttt ccgggcaggc agcccgaatg cccgggtccg tcgactgggt gctgggcgtg
      841 tacggcctgc atcaggaagt ggacctcgag cgcgactaca ccttcttctt cggcgagccg
      901 ttcgccagcc gtttcgaggt ggaccggctg gccgtctacg gcgagctcgg cgtgcagatg
      961 tcgcagcgca tccgggcctc tttggggctt cggttcgagc accacgagtc cgactactcg
     1021 gattccgagg cggtggcggc cgatccgaag gacgacatgg tcggcggtcg gttggtgctg
     1081 gaagcggacg tcgcggacga cgccatggtc tatttcagcg ccagccgcgg ctacaaggcc
     1141 ggcggcttca ataccagcgg caccctggag cgcgagctgc gggatttcga tcccgaaaag
     1201 ctctggaact acgaggtggg cgtgaagggg acgtcggccg acggccggct ccaagcgcgc
     1261 gtcgcggcgt ttctgatgaa aagggacgac gttcaggtgt ccactttcat cgccgtgccc
     1321 cgcgaagacg gcagcaccga gttcatcgac ctcatcgaca acgcggccga aggcaccaac
     1381 gccgggctgg aagccgaggt cctctggatg cccaccgagc agctagaggt gtttgccagc
     1441 ttcggctggc tcgataccga gttcgacgat ttcatcaacg ccgcgggaga ggatctcgat
     1501 ggcgaggagc aggctcacgc gcccggctac cagtttcacg tctggggcga gtatcgattc
     1561 gcggacggct ggttcctgcg cctggcggtg gagggcaggg acgcttttta cttcaccgac
     1621 ggccgccggt tcgcgcccaa cgccgaagat ctgaagtcgg acagctacga gctggtgcat
     1681 gcctctgcgg gttacgaagc agaccggtgg cgggtgtccg cctggggccg caatctgacg
     1741 gacgaggact acgccgtacg aggcttctat ttcggcaacg atccgcggga tttctacacg
     1801 gcgcgcggct tcacccagct cggcgagcct cgccgttacg gaatcaccct ccagctggag
     1861 ctctgaccgt cgtcaatccc gtgcaggccg cgtcagggcg cggcggcagt ctctgcaagc
     1921 tgctgggaga tcagatagtc gaccacgtcg agcgctcttt tgcgccccag gtccatgttc
     1981 accacgtacc tgtcgtccac gaccagggtg gggacgctgg agatgacgat gttgcgctgg
     2041 atggcgtctg cttcgcgcag gtcgccgcgc acttccggtg agttgaacat ccggagaaat
     2101 tcggctcggg taacgccatt gccgtctacg aaatcggcca tggattccgg cgttgcgaac
     2161 tgccgagcgt tatcgtgaat ggctcggaac agacgcatgt ggttctgctc cagcacgtcc
     2221 aggcgcttca acgtgaaata ggtctgcgcc agcagctgcc aggccggcga gaaagccact
     2281 ggtgagcgct cgaaggcggt ccgttccggc tgcgtcgctc gccactcgtc gagcagcggc
     2341 tcgaaattcc tgcagtggat gcagccgtag gagaaatact cgcgcaccat gatgggcgcg
     2401 cccgcccgcc gtctgggcgg gttctcgatg acccggtaat gctcgccggc cagaaactcg
     2461 ccttcgccca ggccggtgct gtagagcgtg ccgtacccca gcaccacgat gccgaccagg
     2521 atggtgaagc cgacgatcag catgcgcacc cgggtcactt tcttgtccga ctttttgcga
     2581 ctggacatgg ggcctcccct gtcgtttgcg ccggagttta cccacagcga cgctggtgtg
     2641 gaaa
//
LOCUS       DBNHKV010000387         7002 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1966377, whole genome shotgun sequence.
ACCESSION   DBNHKV010000387 DBNHKV010000000
VERSION     DBNHKV010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7002
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1966377"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="AC16PP_12395"
     CDS             <1..184
                     /locus_tag="AC16PP_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6482475.1"
                     /translation="VHGNERGLRNLLEGYRSRLHRVDYRLYTEGRHEMLNEVNRHEVV
                     DDLIAWLNERMGPAQS"
     gene            complement(175..1173)
                     /locus_tag="AC16PP_12400"
     CDS             complement(175..1173)
                     /locus_tag="AC16PP_12400"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR6482476.1"
                     /translation="MPRYVMLMRYHSAGMRAIESNPQTLYEIHDAIARWEAKVLESYR
                     LLGEWDQCTIFEAPDNFKAYRATLAQELGTTADTEILPAIDFDLFSRLVSTEAGSVGP
                     HRWQITWWARIGRRLFRWHAYDRFANRYCKPLTVTGLHKFDTIKGPCIIVANHTSHMD
                     ALVLNASLPNRIRWNVYSGAAADRWFVKGRKELVMQPWYQSLAMGTFPIQRGGGSKAL
                     DYPKWLLENGCNLIIFPEGTRSTSRSMAKFRHGVSVLALEKNVPVVPVFLTGLKAMRP
                     KGSREIFPGPAGANVLDPIYFEEGTTVPDATRRIYDALNEVHLKVGEFGDEAARQD"
     gene            complement(1177..1482)
                     /locus_tag="AC16PP_12405"
     CDS             complement(1177..1482)
                     /locus_tag="AC16PP_12405"
                     /inference="COORDINATES: protein motif:HMM:NF020317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR6482477.1"
                     /translation="MATYIMLSTLTDEGRKTLKQRPERLKEVNKEVESMGARVVSQYA
                     VLGGYDFVNIIEAPTNEVMSRISMELGARGTIKVTTMPGMTIESFVDMLEGKTTSGN"
     gene            1696..2091
                     /locus_tag="AC16PP_12410"
                     /pseudo
     CDS             1696..2091
                     /locus_tag="AC16PP_12410"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
     gene            2143..2565
                     /locus_tag="AC16PP_12415"
                     /pseudo
     CDS             2143..2565
                     /locus_tag="AC16PP_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
     gene            complement(2570..4315)
                     /locus_tag="AC16PP_12420"
     CDS             complement(2570..4315)
                     /locus_tag="AC16PP_12420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHA/PHB synthase family protein"
                     /protein_id="HIR6482478.1"
                     /translation="MSADTNTDTEPNRQPSASDTPGAGSDAFSDEIADRTEGIAAASA
                     FGVDTRAIMDAWLSIANEATTHPRAVLRAATKLGRELGSIVMGSSELAPAPEDKRFSD
                     AAWEANPIFRRIGQSYVAWTRSLDGWLDESGLEGIERERARFVLDVFKDVLAPVNSLA
                     GNPEALKKLRASDGGSLTRGLKNFLDDLRHNHGYPAVADRHAFKVGKDVACTRGAVVY
                     RNELFELIQYRPTTPEVSPVPVLYVFSQVNRFYLGDLTPDRSLFKRLLDAGIPVFAIS
                     WRNPTVEERDWNLDTYADGVITAITVMQQITKQPKIHLMGLCAGGLVAAAAAGVLAAR
                     REDWISTLSLFVNVLDYRPGDSEFGLFVSERSVDAQKAVVRGRGVFAERDVFEMFALL
                     RMEENVMSFFRSNYLMGEEPLKHPLLFWSMDYTRVPAEMQCDFLNLAQYNKLAKKEMR
                     ILGKRVDLTRIDYPVYVMAGTTDHITPWKACFRSTQLFGGDVAFVLTNQNHTQTISSR
                     EDNRHLKHWVVDNPSDNPDRAMRLATEHEGSWTRHWIGWLDDHSGERVKAPRRMGSGK
                     NPILGEAPGLYVLES"
     gene            complement(4564..4911)
                     /locus_tag="AC16PP_12425"
     CDS             complement(4564..4911)
                     /locus_tag="AC16PP_12425"
                     /inference="COORDINATES: protein motif:HMM:NF020918.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HIR6482479.1"
                     /translation="MSMIEKQAKLGRELLELNVDTVRKFAELSSENFRRYMELNQSYA
                     QKLPEVREVGAFVELQRDYGQSLWDGVRDDFKARGDIVRTAVEHTGSLIRGAFGSEPE
                     VAEGQAEAAAEAA"
     gene            complement(5041..6552)
                     /locus_tag="AC16PP_12430"
     CDS             complement(5041..6552)
                     /locus_tag="AC16PP_12430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT/MGAT family O-acyltransferase"
                     /protein_id="HIR6482480.1"
                     /translation="MYKLGALDAGFLYNETERCPHHIASVQVLEPLPGTVPGEFVARL
                     KALLLERVHLVPYFTNKLQRAPFDLDHPVWVRDGDFDIDHHVRTIEVPAPGGREEFEA
                     TIAELHATPLDMSRPLWDLWVLTGLEGGRYAYYNRAHHACLDGMAGQKMIETIMDVTP
                     EPRKVTAPPLGFFAAQSETPTNLLVGALENFARHQVKQSSIWMNQLETTTRLIQRAMD
                     PSKGLGAAAEVAPRTRFNRTIERSRTYASGELSLADVKAVAKATGTKVNDVVLAVIGV
                     ALRRYLRRKGELPSKPLIAGCPVSLRQAGDESTNNQVTMMLVSCASDEADPIRRLLKV
                     AASSAQAKGLTADTAGSYDPDPALPGLPNVLAGMAQLIDRYNLADLDGVPLPCNLVVS
                     NVPGPVTQLYSNGAKMLTHYPVSLPVHGQGLNITVQSYLDEMFFAVTACAKALPDAGR
                     LRDDMLEAFAELREAMGPSDAMQHDAANERAPLPSRPEVVDGGSGAEIHDRAA"
BASE COUNT         1313 a         2209 c         2148 g         1332 t
ORIGIN      
        1 ggttcacgga aacgagcgcg ggctgcgcaa cctgctcgag gggtaccgct cccgcctcca
       61 ccgggtcgac taccgcctct acaccgaggg gcgccacgag atgctcaacg aggtcaaccg
      121 gcacgaggtg gtcgacgacc tgatcgcctg gctgaacgaa cgcatgggcc ccgctcagtc
      181 ctgacgcgcc gcctcgtcgc cgaactctcc aaccttcaga tggacctcgt tgagggcgtc
      241 gtaaatgcgc cgggtggcat ccggaaccgt ggtcccttcc tcgaagtaga tgggatcgag
      301 cacgttggcg cctgcaggac cgggaaaaat ctcgcgggag cccttgggcc gcatggcttt
      361 gaggccggtg agaaaaaccg ggacgacggg cacgtttttc tccagcgcca gaacggacac
      421 cccgtggcga aacttggcca tgctccggct ggtggagcgc gtgccctcag gaaagatgat
      481 gaggttacag ccgttttcga gcagccactt gggatagtcc agcgccttcg aaccgccgcc
      541 tcgctggatc ggaaacgtgc ccatggccag agactggtac cagggctgca tcaccagctc
      601 cttccggcct ttcacgaacc agcgatcggc cgccgctccg gagtagacgt tccagcgaat
      661 acgattcggc agtgaggcgt tgagtaccag cgcgtccatg tggctcgtat ggttcgccac
      721 gatgatgcac ggacctttga tggtgtcgaa cttgtgaagg ccggtgaccg tcaggggttt
      781 gcagtagcga ttggcaaaac ggtcgtaggc atgccagcgg aagaggcgtc ggccgatcct
      841 ggcccaccag gtgatctgcc atcgatgggg cccgacgctg ccggcttccg tgctgaccag
      901 ccgggaaaag aggtcgaagt cgatggcggg cagaatttcc gtatctgccg tggtgccgag
      961 ttcctgagca agggtggccc gatacgcctt gaagttgtcg ggcgcctcga aaatcgtgca
     1021 ctgatcccac tcacccagaa gccgatagct ttccagcacc ttggcttccc agcgcgcgat
     1081 ggcgtcgtga atctcgtaca gggtctgggg gttcgactcg attgctcgca tgccggccga
     1141 gtgataacgc atgagcatca cgtatcgagg catgattcag ttgccgctgg tggtctttcc
     1201 ctcgagcatg tcgacaaacg actcgatcgt catgccgggc atcgtggtca ccttgatggt
     1261 gcctcgtgct ccaagctcca tggagatgcg cgacatgacc tcgttggtgg gggcctcgat
     1321 gatgttgacg aaatcgtatc ccccgagcac ggcatactgc gaaaccaccc gggcccccat
     1381 ggattccact tccttgttca cctctttcag gcgctcgggg cgctgcttca aggtcttgcg
     1441 cccttcgtcg gtcagcgtgc tgagcatgat gtaagtcgcc acgatgattc cccgctattg
     1501 ctgcgaatag catacgcctt taccggtcac gcgccagcgt tgtgcctttg ccgacgggcg
     1561 tttagcatgg tttgaggttt cagcagtccc ccggccattg gccgaggccg ccagggctga
     1621 agcctgattc gccacgggaa cgggttctcg tgaaacatgc tgggggcaga tggaaacatg
     1681 agctttacca aagtaatggc cgccgtcgac tgggacgaag cgagcgccgc ggtagtgatc
     1741 gagaccgccc ggagagcagc cggcggccgg ccggtggaga tcgttcacgt gctggatcag
     1801 gggtattacc tcaacaccat cgatcccgcc tccgcctcca tcgaagatct ccgcgccaag
     1861 gcctcctccg aggccgaggc ttatctgcag ggtctctgcg accggcatgg ggggacgtat
     1921 gccctgctcg aggggtatgc ggcaacgatg ctgcacgaac acgccgagag cggcgggcat
     1981 gacctgctgg tggtcggaag tcacggccgc cacggactca agctgcttct gggatccacc
     2041 gccaacgccg tgctgcatgg caccaaccag cacgttctgg ccgtccgaat tcccggacgc
     2101 ggcgaggaag ccgaggagtt ttcccacgcc taccgtcgat tgatcgcctg cgtcgatctg
     2161 accgacgagt caagccaggt tctcgacatt gcgcagacac tggcgtcaga acacggtgcg
     2221 gagatgcacg tggcccacgt gatcaagccg ctgcagcagg cctacgccgg ggtcaaccca
     2281 tcgacgatgt ccgacgtggg aattcagttc gagcacgaag ccgacgccca ggcccgggac
     2341 gagctcgcga gaattgccga tgtccacggg ctggcggccg accggctgca cgtgcggcac
     2401 ggcgccccgg ctgcggagat ccacgctctc gtcaaagaac ttggggcgga tctggtggtg
     2461 atcggaaccc acggcaagca cggcgtgcaa ctgctgcttg ggtccaccgc caacgcgacc
     2521 cttcacggcg caacctgcga caccctggcc gtgaggatcc gctgaggctt caggactcca
     2581 gcacgtacag accgggggcc tcccccaaaa tggggttctt gcccgagccc atgcgccgtg
     2641 gcgctttgac cctttccccg ctgtgatcgt cgagccagcc gatccagtgt ctcgtccatg
     2701 acccctcgtg ctcggtggca agccgcatgg cccgatccgg attatccgac gggttgtcga
     2761 cgacccagtg cttgaggtgt cggttgtcct cccggctgga gatcgtctgg gtgtggttct
     2821 ggttggtcag gacgaacgcc acgtcgccac caaacagctg ggtgctccgg aaacaggctt
     2881 tccagggcgt gatgtggtcc gtggttccgg ccatgacgta gaccggatag tcgatcctgg
     2941 tgaggtcgac ccgcttgccg aggatgcgca tctccttctt ggcgagcttg ttgtactggg
     3001 ccaggttcag gaaatcgcac tgcatctccg ccggcacgcg ggtgtagtcc atggaccaga
     3061 acagcagcgg atgctttaag ggctcctccc ccatgagata gttgctgcgg aagaagctca
     3121 tcacgttctc ttccatgcgc agcagggcaa acatctcgaa cacgtcccgc tccgcgaaga
     3181 cgccgcgccc gcggacgacg gctttctgag cgtccaccga acgctcgctg acgaacagcc
     3241 cgaactcgct gtcgcccggc cggtaatcga gcacgttgac gaacagggaa agggtgctga
     3301 tccagtcctc gcggcgcgcc gccaataccc cggccgccgc tgcggccacc aatcctccgg
     3361 cacacagacc catgaggtgg atcttgggct gcttcgtgat ctgctgcatg acggtgatgg
     3421 ccgtgatcac gccgtcagca taggtatcga ggttccagtc ccgctcctct acggtggggt
     3481 tgcgccagct gatggcgaat accggaatgc cggcatcgag cagccgcttg aagaggctgc
     3541 gatccggagt cagatcaccc aggtagaagc ggttcacctg gctgaacacg tacagcacag
     3601 gcaccggcga gacctccggg gtggtgggcc gatactggat cagctcgaag agctcgttcc
     3661 gatacaccac cgcgcccctg gtgcaggcca catccttgcc gaccttgaag gcgtgccggt
     3721 cggctaccgc ggggtagccg tggttgtgcc ggagatcgtc cagaaagttt ttcagcccgc
     3781 gagtgaggct acccccatca ctggcgcgca gctttttcag cgcctcgggg tttccggcca
     3841 ggctgttcac cggagccagc acgtccttga agacgtccag aacgaatcgc gcacgctcgc
     3901 gttcgatgcc ctcgagtccc gactcatcga gccaaccgtc cagggatcgg gtccatgcaa
     3961 cgtaggactg tccgatcctt cggaaaatgg ggttggcttc ccaagccgca tcactgaaac
     4021 gcttgtcttc cggcgccggc gccagctcgc tggatcccat cacgatgctg cccagctcgc
     4081 gacctagctt ggttgcggca cgtagaacgg cgcggggatg agtggtcgct tcgttcgcga
     4141 tgctcagcca ggcatccatg atggccctgg tatccacgcc aaacgcgctt gccgccgcaa
     4201 tcccttccgt ccgatcggcg atctcgtcgg agaacgcatc gctcccggca cccggggtat
     4261 ccgatgctga tggctgcctg ttcggctcgg tgtctgtgtt ggtgtcagcg ctcacgctag
     4321 tcacctcgtc tgggtcgcat ccatggtcca catcttagac cacctgatga tggttgaaag
     4381 aaaagcatcc ggccatcccg gagattcgga cgtcaacccg gcgggttgaa tcgacggcga
     4441 caggacggca tctcagcccc caccaccgaa aaaaaaagcc ggctccgtag caacacagag
     4501 ccggcctttt catctgagcg ccgggggaac agcagatccc gcggcatgct cagtggagat
     4561 gcactacgct gcttcggctg cggcttctgc ctgaccttcg gctacctcag gctcggagcc
     4621 gaacgcgccg cgaatcaggg agcccgtgtg ctccacggcg gtgcgcacga tgtcgccacg
     4681 cgccttgaag tcgtcgcgaa cgccgtccca gaggctctgg ccatagtcgc gctgcagttc
     4741 cacgaaagcg ccgacctcac gaacttccgg aagcttctgc gcgtagctct gattgagctc
     4801 catgtacctg cggaagttct ccgaggacaa ctcagcgaac ttgcgaacgg tgtccacgtt
     4861 gagttccagg agctcgcggc caagcttggc ctgtttctcg atcatcgaca tctcatttct
     4921 cctcggctac tagccatcga atcggctaat ctgttgcgat gcacaacgaa ttgtgcacca
     4981 caacatattg cattgcaaca tttatttaat ttcaggagtc cacgaccctc ctgtacccgg
     5041 tcacgcagcc cgatcatgga tttcggcccc ggagccgcca tcgacaacct ccggacggga
     5101 cggaagaggc gcgcgctcgt ttgccgcgtc gtgctgcatt gcgtcgctcg ggcccatggc
     5161 ctcgcgcaac tccgcaaacg cctcgagcat gtcgtcgcgc aaacggcccg cgtcgggcag
     5221 cgctttcgcg cacgccgtca ccgcgaagaa catctcatcc agatagctct gcaccgtgat
     5281 gttgagcccc tggccgtgca cgggcaggga gaccggatag tgggtcagca tcttggcgcc
     5341 gttcgagtag agctgggtga cgggcccggg aacattagac accaccaggt tgcacggcaa
     5401 cggcacgccg tcgaggtcgg caaggttgta ccgatcgatg agttgggcca tcccggccag
     5461 cacgtttggg aggcccggca atgccgggtc cggatcgtag ctgcccgcag tgtcagcggt
     5521 gagccctttc gcctgggccg aggacgccgc gaccttgagg agtcggcgga tcggatcggc
     5581 ctcatcggaa gcacagctga cgagcatcat ggtgacctga ttgttggtcg actcatcgcc
     5641 cgcctgccgc aacgacacgg ggcaaccggc gatcagaggc ttggacggga gttccccctt
     5701 tcggcgcagg tagcgccgca gagcgacccc aatgacggcc aggaccacgt cgttgacctt
     5761 ggtgcccgtg gcttttgcca cggccttgac gtccgccagg gacagctctc ccgaagcata
     5821 ggtcctggag cgctctatgg ttcggttgaa gcgcgttcgc ggtgccacct ccgcagccgc
     5881 ccccaggccc ttgctcggat ccatggctcg ctggatcagc cgcgtcgtcg tctccagctg
     5941 attcatccag atggaagact gctttacctg atgccgcgcg aagttttcca gcgcgccgac
     6001 cagcagattg gtgggcgtct cggactgagc ggcgaagaag cccagagggg gcgccgtgac
     6061 cttacggggc tccggcgtca cgtccatgat ggtttcaatc atcttctgcc cggccatccc
     6121 gtcgaggcag gcgtggtggg cgcggttgta gtaagcgtag cggcctccct cgagaccggt
     6181 cagcacccac aggtcccaca gaggtcggct catgtccagc ggcgtcgcgt gaagctcggc
     6241 aatcgtcgct tcgaactcct cccggcctcc cggagcgggg acctcgatag tgcggacgtg
     6301 atgatcgatg tcgaaatccc catcacgcac ccataccgga tgatcgagat cgaacggcgc
     6361 cctttggagc ttgttggtga agtacggcac aagatggacg cgctccagca acagcgcctt
     6421 gagccgagcc acgaactcgc cgggaaccgt tcccggcagc ggctcgagca cctgaaccga
     6481 agcgatgtga tgcgggcatc tttcggtttc gttgtagaga aaacccgcgt ctaaagcacc
     6541 cagcttgtac acttgaaacc tccatgattg gtgtcacgtt gacgacggcc gccccgattt
     6601 ccggtaggcc aacgagggca cggaatccgc ggcgtccact gacttctcag caaaccccgt
     6661 gccatcaggc aaaaatcgtt taacccgttg aatttaaata acattattgt acgatccgct
     6721 gtgcccgtcc tggtccacgt tggtgcgcag cgctgatcgc gcaccaaaac cgcgccggtt
     6781 ccgcgaaccg atggcgccga catggcccca agacatggcc ccaaaatagt cgcaatatgt
     6841 tgcgttgcaa tatcaggaaa aagcgcgcag tttagcgtca gattgctaca cgtcaattac
     6901 cgggagcgaa attatccgtc agagtgccta ttttggggct tcggtcagag gcgaccgtgt
     6961 ctattgcaac gcagcataaa cgtgttgacc gaggtaggtt ta
//
LOCUS       DBNHKV010000388        10108 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_904859, whole genome shotgun sequence.
ACCESSION   DBNHKV010000388 DBNHKV010000000
VERSION     DBNHKV010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10108
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_904859"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="AC16PP_12435"
     CDS             <1..917
                     /locus_tag="AC16PP_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR6482481.1"
                     /translation="PAAGEVLLAVHACGVCRTDLHVVDGDIVDGRYPVVPGHQIVGVV
                     EQAPDGDLQPGQRVGVPWLGHTCGTCVFCLAGQENLCDSAEFTGYHRQGGFAEYAVAD
                     ARFCFPIPESYPDVQAAPLLCAGLIGSRALGMVGDARKLGFYGFGNAAHILTQIAVWE
                     GREVSAFTRPGDAAGQRFARGLGAVWAGDASTPPPEPLDAAIIFAPDGALVPAALKHV
                     RKGGVVVCAGIHMSDIPSFPYADLWGERIIRSVANLTREDGVRLLEVAPRVPVRTEVQ
                     VFPLERTADALDALREGRVEGGAVVQVR"
     gene            complement(914..1960)
                     /locus_tag="AC16PP_12440"
     CDS             complement(914..1960)
                     /locus_tag="AC16PP_12440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6482482.1"
                     /translation="MRISMLLSYAGGFKEAVKEVAELEKAGLDLVWVPEAYSFDAPSA
                     MGYLAANTERVTIASGILPIYSRTPSLLAMTAAGIDYLSDGRCMLGLGASGPQVIEGF
                     HGVPYVAPVSRIREIIEICRKVWRRERLQHDGRYYQIPLPQNKGTGLGKPLKLINHPV
                     REEIPIAIAALGAKSVEMTAELADAWLPAFYTADAADAVWGDALRAGAAKRDPGRAPL
                     EVFAGGAVAIGEGLEDLRNLARPQAALYIGGMGARQKNFYNDIFSRSGYAQEAKLIQD
                     LYLSGDKKGAEAAIPESYLANNSLIGPEGFVRDRLQALSESGVTALNVGFMGRTSQER
                     IRSCEQLRKLVDTL"
     gene            2085..3302
                     /locus_tag="AC16PP_12445"
     CDS             2085..3302
                     /locus_tag="AC16PP_12445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR6482483.1"
                     /translation="MDREFDVIVWGATGFTGRLVAEYLNERYGLGGSVAWAIAGRNQG
                     KLDAVRQEIGAPGLSTILADGNDEASLAAMAERARVICTTVGPYALYGSRLVAACVSR
                     GTHYCDLTGEVHWMRRMIDNHHLSAQQAGVRIVHTCGFDSIPSDIGVLCVQREMKSRH
                     GEVATEVKSRVRGMRGGFSGGTVASMLNMMEESEMDPGVREVMNDPYGLNPAGERHGL
                     DGPEHSVPEYDPDFGAWTTPFVMAAINTKVVRRSNALSGYAYGRDFRYDEAMLTGFGP
                     WGFPLAAAMSVGSTAFNAAAGVSALRGLLARVLPEPGEGPSAAARERGFFDIEMLGKS
                     RRSGARVKVRIRGDRDPGYGSTSRMLGESAVCLARDDLETPTGVLTPAVAMGEALIGR
                     LGNNAGVTFEVMP"
     gene            3346..4425
                     /locus_tag="AC16PP_12450"
     CDS             3346..4425
                     /locus_tag="AC16PP_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482484.1"
                     /translation="MSETGPAPQERGKSNILLRIATWLITAGCFYLVYGRTAEAAARD
                     GLTVLEYLARFFGEANWLAWLAVMVPYSVFFFVVDAHVTWRVIRWFNAPEIRLGNVLP
                     IRASAYILSLVNEQVGKGAMSLYLYRRYKVPALAAISSMLLLGLMEIYQLLVFSSVGV
                     FLGFEVVQAASTELPLDKILPGVFAVAAIYLPLHIAYFRGHLLKGSTVREKSLVRAFR
                     EARPIHYLLILLFKAPLLIGAIVVYTIALNLFNVDVSFAQMLAFLPVIFLAAALPLPF
                     HAGALLFWTILFPDFPEVGAFSLVMHTFFVLFNALIGVVFLPIANRELFGSEQPGSGT
                     VAHGNDAGDDEHPGQPLDQGGAGPR"
     gene            complement(4315..4842)
                     /locus_tag="AC16PP_12455"
     CDS             complement(4315..4842)
                     /locus_tag="AC16PP_12455"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR6482485.1"
                     /translation="MWFTWANLLTTVRLALAVPCAMAVVSGDWPLAAALFALAVITDL
                     LDGPLARRLNHASPVGGLFDHATDALFVTFALGALATAGYLTWALPCLVAAAFIQYAL
                     DSRALAGKHLRTSYLGRMNGIAYFFAAGIPIVKEGLALPWPSAALIQGLAWVLVVTSI
                     VSMGDRARAWLLRSE"
     gene            4986..5885
                     /locus_tag="AC16PP_12460"
     CDS             4986..5885
                     /locus_tag="AC16PP_12460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6482486.1"
                     /translation="MIVVTGANGNLGRRLLQSLSRRAPLRAVVRSERAAEQIRALTLE
                     PEPEICVVDYQDAAAMTGALAGAEYVVHLVGIIKETAASTYVDAHERTSEVLCQAADS
                     AGALRIVYLSIVGSEPGSSNPCLASKGAAEAILLAAATPALVVRVPMVLGEGDYAAGA
                     LARRARKGWNVLLRGASLEQPIYAGDVIEAVKAGLGSAPAPAALDDAVLDLAGPVSLS
                     RSDLTRRSASILGRSTRVISLPLFLGLLLAGLLERLLANPPVTAAMLRVLDHDDRLDP
                     EPACHVLGIELTPLDEVLARCLR"
     gene            6018..7709
                     /locus_tag="AC16PP_12465"
     CDS             6018..7709
                     /locus_tag="AC16PP_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482487.1"
                     /translation="MQELSNSGLTRSADNAPEETGSGPARAPGKGRELDDLKQELAKW
                     QERVPKLAAALRERTEHASELQAQIEKLEANHRASSDQAAAASKERQQRVDELHSQLQ
                     AQQAKHQELQSELQARGAQIADLRLEVADWKEKHQHLVEAGEQASGPSEEDQAALEAL
                     KRENASLAEALAEKTRLAEERAREVASLREDAESLSSRNATLFETTEFASKQLDSLGD
                     NLGELRDELNARSEALREKTARIEALEGQLSERETELEVFREKMESAGVYEEMALELE
                     ESLAAERQEVERLQQCVKTANRITSEREYERRALSEELETLMERNQELEAAAEAGGDQ
                     DPELEQQRDLMLSLEEELAQAKDEQVALEKSHAKTLAEKERQLSELREELDAAADRAG
                     KLAADESIGEAQPEHPAQDKKVEVLQQQVTTLEELIRERTEELNELKWRAQLADGERE
                     AITEDDAKMMLVLNQQLGEARESNDRLIAVIGRLEERLAASDLGPAGDDFTRMRGIGD
                     KLAAQLNELGIRTFGQIADIDLAALENDHHPLAAFKHRIEKDDWVAQARELAGRG"
     gene            7847..8503
                     /locus_tag="AC16PP_12470"
     CDS             7847..8503
                     /locus_tag="AC16PP_12470"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6482488.1"
                     /translation="MSHSSVPLVLRRTTSFLFMVVLVPMGLGVGSAVADVAAGKAAYM
                     VCASCHGQNGEGNAALNAPRIAGQEPWYISRQLAAYRAGHRGTAPGDIYGMQMRPMAM
                     TSADPATEANLIEYIGTLSAAPATATIEGDAAAGQNGYAVCVACHGANGEGNQALGGP
                     KLAGQNDWYLVRQIKNYQSGLRGYDPADTYGMQMKPMAGVLADDKAINDVVAYINTLR
                     "
     gene            8506..9552
                     /gene="alr"
                     /locus_tag="AC16PP_12475"
     CDS             8506..9552
                     /gene="alr"
                     /locus_tag="AC16PP_12475"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR6482489.1"
                     /translation="MRGRLHVDLAALAANYRSFSSAAEAAGGTAGGVVKADAYGLGAA
                     RVAATLQSEGCAEFFVATVEEGAALRAILPEAIIYVFEGATGATAEVLASNDLVPVLN
                     HRGQLEAWQAYRERPAAVHVDTGMNRLGFPADVDAVQFAGMNLSLLLTHLACADVPDD
                     PMNHEQVERFRRAATSFPGVRTSIGNSAGMLADASCCGDLGRPGVGLYGGNPFSDRRN
                     PMRRVAALQGQVLQVRTCSAGSPLGYGATYVAPADLRVAVVGLGYADGLPRLISGRGC
                     AAVGGRRCALLGRVSMDLTLVDVTGLEVQAGDWIEMFGDTVSVDEVAGWAETIAYEIL
                     TGIGDRVERVYSGD"
     gene            complement(9537..>10108)
                     /locus_tag="AC16PP_12480"
     CDS             complement(9537..>10108)
                     /locus_tag="AC16PP_12480"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6482490.1"
                     /translation="EAGGAPGSIQSAAELLVADEAALGCHREVTAADPNPEPCDVFLR
                     LTADSLDETLRQAAAHNNRGLILTRLGGLEAALADFEAALALAPGLGAAEVNRGTALL
                     HMGLYPDALKAFENVIVNDEEHRATARFNRSFVYRALGELDLAAADLASAAQTDLPVS
                     RSREPIPLQAGPAAEPLDRPMELEGQSPE"
BASE COUNT         1650 a         3229 c         3479 g         1750 t
ORIGIN      
        1 gacccgcggc gggcgaggtt ctactggccg tgcacgcctg cggcgtctgc cgcaccgatc
       61 tccacgttgt ggacggggac atcgtcgacg gccgctatcc cgtcgtgccc gggcaccaga
      121 tcgtcggggt ggtcgagcag gcgccggacg gcgatctgca accgggacag cgggtgggcg
      181 tcccgtggct cggccatacc tgcggcacct gcgttttctg ccttgcgggc caggagaacc
      241 tgtgcgattc ggccgaattc accggatacc atcgccaggg tggattcgcg gagtacgcgg
      301 tcgccgacgc ccgcttctgt ttccccatcc cggaaagcta tccggacgtt caggcggcgc
      361 cgctgctgtg cgcggggctg atcggctccc gggccctggg aatggttggg gatgctcgaa
      421 agctgggttt ctacgggttc gggaatgccg cccacatcct cacccagatt gcggtatggg
      481 aggggcgcga agtgtccgcg tttacccgcc cgggggatgc ggccggccag cgattcgcga
      541 ggggtctggg tgctgtgtgg gcgggtgatg cgtcgacgcc ccctccggag cccctggacg
      601 ccgccatcat cttcgcgccg gacggggcgc tggtgccggc ggccttgaag catgtgcgca
      661 agggtggggt ggtggtctgc gcgggcattc acatgtccga catcccttcg ttcccctacg
      721 ccgatctctg gggcgagcgc atcatccgct cggtggccaa cctgacccgc gaagacggcg
      781 tgcggttgct ggaggtggcg ccccgcgtcc cggtccgcac cgaggtgcag gttttcccgc
      841 tcgagcggac ggcggatgcg ctcgatgctc tgcgggaagg ccgcgtcgag ggaggcgccg
      901 tcgtccaggt gcgctagagc gtgtccacca gcttgcgcag ctgctcgcag ctgcgaatcc
      961 gctcttgtga cgttctgccc atgaagccca cgttcagggc ggtcactcct gattcgctca
     1021 gcgcttgcag gcggtctcga acgaaaccct cgggcccgat gagcgagttg ttggccaggt
     1081 agctctcggg gatagcggcc tcggcccctt tcttgtcccc ggacaggtac aggtcctgga
     1141 tcagcttggc ctcttgtgcg tacccggagc ggctgaagat gtcgttgtag aaattcttct
     1201 gtcgcgcgcc catgccgccg atgtaaagcg ctgcctgggg gcgcgccaga ttccgcaggt
     1261 cttcgaggcc ctcgccgatg gccaccgcgc cgccggcgaa cacttccagc ggcgccctgc
     1321 ccggatcccg cttcgcggct ccggcgcgaa gcgcatcgcc ccacaccgcg tccgcggcat
     1381 cggcggtgta gaaggcggga agccaggcat cggcgagctc cgccgtcatc tccacgctct
     1441 tggcgcctag tgcggcgatg gcgatgggaa tctcttcgcg gaccgggtgg ttgatgagct
     1501 tcagcggctt gcccaggccg gtgcctttgt tctgcggcag cggaatctgg tagtagcgcc
     1561 cgtcgtgctg cagacgctcg cggcgccaaa ccttccggca gatctcgatg atttcccgga
     1621 ttcgggagac gggtgccacg tagggcacgc cgtgaaagcc ttcgatgacc tgaggcccag
     1681 acgcgccgag cccgagcatg cagcggccgt ccgagaggta gtctatgcct gctgccgtca
     1741 tggccagcag agacggtgtc cgggagtaga tgggcaggat gccggaggcg atggtgacgc
     1801 gctcggtgtt tgccgccagg tagcccatgg cgctgggcgc gtcgaaggag tacgcctcgg
     1861 gaacccagac cagatcgaga ccggctttct ccagctcggc gacttctttg accgcttcct
     1921 tgaaaccgcc ggcatagctc agcagcatgg atatacgcat gttcaacccc ttgagaaggt
     1981 gatggttcgt tggtcgccgg cccggccgcg gagtatgctt ggcggctgcc gcccctttgc
     2041 caccggcgtg gcggtcgatg ttaacagaat gaggatcact gccgttggat cgggaatttg
     2101 acgtcatcgt ctggggggcc accggtttca ccgggcgcct cgtagccgag tacctcaacg
     2161 agcgctacgg attgggcgga agcgtcgcct gggcgatcgc cgggcgaaat cagggcaagc
     2221 tcgatgcggt gcgtcaggag atcggcgcgc cgggactgtc cacgatcctg gccgacggca
     2281 acgacgaggc atcgctcgcc gccatggccg agcgcgcccg cgtcatctgc acgacggtgg
     2341 ggccctatgc tctgtatggc agccggctcg ttgctgcgtg cgtcagccgg ggaacccact
     2401 actgcgatct gaccggcgag gtccactgga tgcgccgaat gatcgacaac catcacctca
     2461 gtgcccagca ggccggggtt cggatcgtcc acacgtgcgg cttcgacagc ataccgtcgg
     2521 acattggcgt gctctgcgtg caacgggaaa tgaaatcccg gcacggtgag gtggccaccg
     2581 aggtcaagtc ccgcgtgcgc ggaatgcgcg gcggcttcag cggcgggacc gtggccagca
     2641 tgctcaacat gatggaagaa agcgagatgg acccgggcgt tcgggaggta atgaacgatc
     2701 cgtatggcct caaccccgcg ggcgagcgtc acggcctcga tgggcccgag cactcggtgc
     2761 ccgaatacga cccggatttc ggcgcatgga ccacaccgtt cgtcatggca gccatcaaca
     2821 ccaaggtcgt tcgccgcagc aacgcgttgt ccggctacgc ctacgggcgc gactttcggt
     2881 acgacgaggc gatgctgacg ggcttcggcc cctggggctt cccgctggcc gcggcgatga
     2941 gcgtcggctc gacggctttc aacgccgccg ccggggtttc tgcgctgcgt ggattgctgg
     3001 cgcgggttct gcccgagccc ggggaggggc cctcggccgc tgcccgcgag cggggattct
     3061 tcgacatcga gatgctcggc aagagccgcc gcagcggtgc gcgggtcaag gtcaggatcc
     3121 gcggggatcg tgatccgggc tacggctcga cctcgcggat gctgggcgag agcgccgtct
     3181 gcctggccag agacgatctg gagacaccca cgggcgtgct taccccggcc gtggccatgg
     3241 gcgaagcgct gatcgggcgt cttggcaaca acgccggcgt gacgttcgag gtgatgccgt
     3301 gaggcgctca cttcgtcgct catctaacga aaggagcgcg tgggcgtgag cgagaccggg
     3361 ccggctcccc aggaacgcgg caaatccaac atcctccttc ggattgcgac ctggctgatt
     3421 actgccggct gcttctatct cgtttacggc cggacggccg aagcggcggc acgtgacggc
     3481 ctgaccgtgt tggaatacct ggcccgcttc ttcggcgagg ccaactggct tgcctggctc
     3541 gcggtcatgg tcccctactc cgttttcttc ttcgttgtcg acgctcacgt gacctggcga
     3601 gtcatccgct ggttcaatgc ccccgaaatc cggctgggca acgtgctgcc gatccgtgcc
     3661 agcgcctaca tcctgtccct ggtcaacgag caggtgggca agggcgcgat gtccctttac
     3721 ctctatcgtc gttacaaagt ccctgctctg gcggccatct ccagcatgct gctgctcggg
     3781 ctgatggaaa tctaccagtt gctggtgttc tcctcggtcg gggtctttct cggattcgag
     3841 gtggtccagg cggcctccac ggagctgccg ctcgacaaga tcctgcccgg cgtgtttgcc
     3901 gtggcggcca tctatctgcc gctgcacatc gcctacttcc ggggacatct actgaaggga
     3961 tcgacggttc gggagaagag cctggtccgg gcattccgtg aagcccggcc cattcactac
     4021 ctgctcattt tgctgttcaa ggcgccgctt ctgatcggtg cgatcgtcgt ctacaccatc
     4081 gccttgaacc tcttcaacgt cgacgtcagc ttcgctcaga tgctcgcctt ccttcccgtg
     4141 atctttctgg ccgcggcgct gccgctgccg ttccatgccg gcgcccttct tttctggacc
     4201 attcttttcc cggactttcc cgaggtcggt gctttcagcc tcgtgatgca caccttcttc
     4261 gtgctgttca acgcgttgat cggggtggtg ttcctgccga tcgcgaatcg ggagctattc
     4321 ggatcggagc agccaggctc gggcacggtc gcccatggaa acgatgctgg tgacgacgag
     4381 cacccaggcc agcccctgga tcagggcggc gctgggccac ggtagggcca gcccctcttt
     4441 aacgatgggt atgccggcgg cgaagaagta ggcgatgccg ttcattcgcc cgagatagct
     4501 cgtccgcagg tgtttgcccg ccagcgcccg ggagtcgagg gcatactgta tgaaagccgc
     4561 agcaaccagg caggggagcg cccaggtcag gtagccggca gtggccaggg cgcccagagc
     4621 gaaggtgacg aagagcgcgt ccgtcgcgtg gtcgaacaag cccccgaccg ggctggcgtg
     4681 gttcaagcgt cgcgcgagcg ggccgtcgag caggtcggtg atcacggcaa gcgcgaacag
     4741 ggctgcggcc agcggccaat caccggagac aacggccatg gcgcacggca ccgcaagcgc
     4801 caggcggacg gtggtgagga gattcgccca ggtgaaccac atggcgcgat tatgctacgc
     4861 cagccaccca tcggtgtcgc cttgctgagc gcgttcgcgc gctccagaat gtggcccgtg
     4921 atgcgcgtcg aacaccagag cctgccggta tcgcccgccg atccatggat cggcaggggc
     4981 tgcccatgat cgtcgtcacc ggagcaaacg gcaatcttgg caggcggctt ctgcagagcc
     5041 tctcccgccg ggccccgctg cgggccgtgg tgcgctcgga gcgggccgcc gagcagatca
     5101 gggctctgac gctggaaccc gaacctgaga tatgcgtggt cgattaccag gatgctgccg
     5161 ccatgaccgg ggcgctcgcg ggggcggaat atgtcgtcca tctcgtaggt atcatcaagg
     5221 agacggcggc cagcacctac gtcgatgctc atgagcgcac ctccgaggtg ctctgccagg
     5281 cggccgactc ggcgggcgcc ttgcggatcg tgtacctgag catcgtcggc tcggaaccgg
     5341 gcagcagcaa tccgtgtctg gcgtcgaagg gggctgcgga agccatcctc ttggccgccg
     5401 ccacgcccgc gctggtggtg agggtgccca tggtgctggg cgagggtgac tacgccgccg
     5461 gcgcgctggc gcggcgcgcg cgaaagggtt ggaacgttct gctcaggggc gcgagcctgg
     5521 agcagcccat ctacgccgga gacgtcatcg aggcggtgaa ggccgggctc ggttctgccc
     5581 ccgctccggc ggccctggac gatgccgtgc tggacctggc cgggcccgtt tccctctcgc
     5641 gcagcgatct tacgcgccgg tccgcgagca tcctgggtcg atccacccgg gtgatatccc
     5701 tgcccctgtt cttggggctg ctgcttgccg gtctgctgga gcgcctgctc gcgaacccgc
     5761 cggtcaccgc tgccatgctg cgggttctcg accatgacga ccgcttggat ccggagcccg
     5821 cctgccacgt cctcggcatc gagctcacgc cgctcgacga ggtcctcgcc cggtgtctgc
     5881 gatgacgaaa ccgtgacgcc cgtcacggcc atctgaattc ccacccagtt cacattcagg
     5941 ctgggcgaat tcaagtagtt ttgcgcggtg acccgagaaa gtcggccaga cggtcctcgg
     6001 agtggatgga tacccgcatg caggaattgt cgaacagcgg cctgactcgg tcagccgata
     6061 atgctccgga ggagaccggt tcggggccgg ctcgtgctcc agggaaggga cgcgagctgg
     6121 acgatctcaa gcaggaactc gccaagtggc aggagcgggt gcctaagctc gccgccgcgc
     6181 tgcgcgagcg cacggagcac gcaagcgaac tgcaggccca gatcgagaag ctcgaggcca
     6241 accaccgtgc ctcgtcggat caggctgccg ctgccagcaa agagcgtcag cagcgggtgg
     6301 acgagctgca cagccagctt caggctcaac aggcaaagca ccaggagctg cagagcgagc
     6361 tgcaggcgcg tggtgcgcag atcgccgacc tccggctgga ggtggccgac tggaaagaaa
     6421 aacaccagca cctcgtcgaa gcgggcgagc aggcgtccgg gccctcggaa gaggaccagg
     6481 cggcgctcga ggcgctcaag cgtgagaacg cgagcctcgc cgaagcgctg gccgagaaga
     6541 cgcggctcgc cgaagagcgc gcgcgggagg tcgcctcgct tcgcgaggac gccgaatcgc
     6601 tctcgagccg caacgcgacg ctcttcgaga ccaccgagtt tgccagcaag cagctcgaca
     6661 gcctcggcga caacctgggc gagctgcgcg acgagctcaa cgctcgcagc gaggcgcttc
     6721 gggaaaaaac cgctcgaatc gaggccctgg aaggacagct cagcgagcgt gaaaccgagc
     6781 tcgaggtctt ccgggagaaa atggagtcag cgggcgttta cgaggaaatg gcgctggagc
     6841 tcgaagagtc gctggccgcc gagcgtcagg aagtcgagcg cctgcagcag tgcgtcaaga
     6901 cggccaaccg catcaccagc gagcgcgagt acgagcgtcg ggcgctgtcc gaggagctcg
     6961 agacgctcat ggaacgcaat caggaactgg aggctgcagc cgaagcgggc ggcgatcagg
     7021 atcctgagct ggagcagcag cgcgatctga tgctcagcct ggaggaagag cttgcccagg
     7081 cgaaagacga acaggtcgcc ctggaaaaat ctcatgcgaa gaccctggcc gagaaagagc
     7141 ggcagctctc cgagctcagg gaagagctgg atgcggccgc cgatcgtgcc ggcaaactcg
     7201 ccgctgacga gtcgattggg gaagcacaac ccgaacaccc ggcccaggac aagaaagtcg
     7261 aggtgctgca gcagcaggta accacgctgg aggagctcat cagagagcga accgaggagc
     7321 tcaacgagct caagtggcgg gctcagctcg ctgacggcga gcgcgaggcg atcaccgagg
     7381 acgacgccaa gatgatgctg gtgctgaacc agcagctcgg cgaagctcgt gagagcaacg
     7441 accgcctcat agccgtcatc ggtcgtttag aggagcggct cgccgcaagc gatctgggtc
     7501 ccgccggcga cgacttcacg cgaatgcgcg gtatcggcga caagctggcc gcgcagctca
     7561 acgagctggg catacgcacc ttcgggcaga ttgccgacat tgatcttgcc gctctcgaga
     7621 acgaccacca tcccctggcg gcgttcaagc atcgaatcga aaaggacgac tgggtcgctc
     7681 aggcccgcga gcttgccggg agaggctgat ggcctcttga gcggctcgtc gccgtgggtc
     7741 cctcgcgggc aaaggacccg gcttgctcac cttgagtttt ccgcgggtaa ctcgctatag
     7801 tctgctgcgc tcgccgccct ttcgattgca gctaccagga ggtttcatgt cgcactcatc
     7861 cgtgccgttg gttttgcgcc gtacgacgag ttttctgttc atggtcgttc tggtcccgat
     7921 ggggctgggt gtgggctcgg cggtcgccga cgtcgccgcc ggcaaggcag cgtacatggt
     7981 ttgcgcgagc tgtcacgggc agaacggcga gggcaacgcg gccctgaacg cgcctcgcat
     8041 cgccggtcag gagccctggt acatcagccg tcagctggcc gcttaccggg ccgggcatcg
     8101 cgggacggcg cccggagata tctacggcat gcagatgcgg cccatggcca tgacctcggc
     8161 ggacccggca accgaggcca atctcatcga gtacatcgga acgctttccg cggcgcctgc
     8221 cacggcgacc atcgaaggcg acgccgctgc cggccagaat ggctacgcgg tgtgcgtggc
     8281 ctgtcacggt gcgaacggcg agggcaatca ggccctggga gggcctaagc tggccggcca
     8341 gaacgactgg tacctcgtgc gccagatcaa gaattatcag agcggcctgc gcgggtacga
     8401 tcccgccgat acctacggca tgcagatgaa gcccatggct ggcgttctgg ctgacgacaa
     8461 agccatcaac gacgtggttg cgtacatcaa cacgctgcgc tgatcgtgcg cggccggctg
     8521 cacgtcgacc tggctgcgct cgccgcaaat taccggtcgt tctccagcgc ggctgaggcc
     8581 gcaggcggaa ccgcgggtgg ggtcgtcaag gccgacgcct acggtctggg cgccgcgagg
     8641 gtggcggcaa ctctgcagtc tgagggttgc gcggagttct tcgtagccac cgtggaggaa
     8701 ggcgccgcgc tgcgggccat cctgccggaa gccatcattt acgttttcga aggcgccacg
     8761 ggggcaaccg cggaagtgct ggcatccaac gacctcgttc ccgtgctcaa ccatcggggt
     8821 cagctcgaag cctggcaggc ttaccgggag cgtcctgccg ccgtccatgt ggataccggt
     8881 atgaaccggc tggggttccc cgcggacgtc gatgcggtgc agtttgcggg gatgaactta
     8941 agccttctgc tcacccatct ggcctgcgcg gacgtgcccg atgaccccat gaatcacgag
     9001 caggtcgagc gcttccggcg cgccgccacg tcgtttccag gtgtacgcac gagcatcggc
     9061 aactccgcgg gcatgttggc cgacgcgtcg tgctgcggtg accttggccg tcccggtgtc
     9121 gggctctacg gcggcaatcc cttctccgac cgacgcaacc ccatgcgccg ggtggccgcc
     9181 ctccaagggc aggtcctgca ggtgaggacg tgctcggctg gcagcccgct gggctatggg
     9241 gcgacctacg ttgcgcccgc cgatctgcgg gtggccgtgg tggggctggg ttacgcggac
     9301 ggcctgccga ggctgatatc gggccgaggc tgcgctgccg tgggtggtcg gcgctgcgcg
     9361 ttgctcggcc gggtttccat ggacctgacc ctggtggacg taaccgggct cgaggttcag
     9421 gccggcgact ggatcgagat gtttggcgac acggtgtccg tcgacgaggt tgccggctgg
     9481 gcggagacca tcgcttacga gattctcacc ggcatcggcg atcgcgtgga gcgcgtctac
     9541 tccggggatt gaccctcgag ctccattggc cggtccaggg gctctgcggc gggtcccgct
     9601 tgcaggggta tcggctcgcg gcttcggctc acgggcaggt ccgtctgcgc ggcagatgcc
     9661 aggtcggcgg cggcgaggtc cagctcgccc agggcccgat acacgaagga gcggttgaac
     9721 cgtgccgttg cgcgatgctc ctcatcgttg acgatgacgt tctcgaacgc tttgagcgcg
     9781 tcggggtaga gccccatgtg aaggagcgcg gttccgcggt tgacctctgc cgcgcccaac
     9841 ccaggggcca gagcgagagc cgcttcgaag tcggctagcg ccgcctccaa ccctcccagc
     9901 ctggtcagga tcagacctcg gttgttgtgc gccgctgcct gccggagagt ctcgtccaag
     9961 ctgtcggcgg tcagtctcag aaaaacgtcg cagggctcgg gattggggtc tgctgccgtg
    10021 acttcgcgat ggcagccgag tgccgcttcg tcggccacga gcagctcggc cgccgactgt
    10081 atcgagccgg gcgcgccgcc agcttccc
//
LOCUS       DBNHKV010000389         4509 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1329990, whole genome shotgun sequence.
ACCESSION   DBNHKV010000389 DBNHKV010000000
VERSION     DBNHKV010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1329990"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..231
                     /locus_tag="AC16PP_12485"
     CDS             52..231
                     /locus_tag="AC16PP_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482491.1"
                     /translation="MVRHSQPSFNSEGDSWTVEAYVANLTDEENEQAIIITQFDNTRF
                     FQRPRTYGLRFRYRM"
     gene            complement(269..2128)
                     /locus_tag="AC16PP_12490"
     CDS             complement(269..2128)
                     /locus_tag="AC16PP_12490"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR6482492.1"
                     /translation="MSNNLIAAAVRPLAGAVMLWSGLGWAQEYSVPVDGDYPKNVYWG
                     DTHLHTRNSPDAYSMGNMNLTPADAYRFAQGQEVVAHNGMRVQLRRPLDFLVVSDHSE
                     YIGGYYRFNVGDPIVTETEAGRQWQGYLDEGGDAVANIYATFVNSMQDPENYPPLPER
                     SRLLIWQDVATTADEYNRPGQFTAFTGYEWTAMIDGNNLHRVVVYKDSADKVSQLPPF
                     SGQDSLDPRELWKALARYEAQTGGEVMAIAHNGNLSNGMMFPDKSVDGKPIDRAYAEL
                     RARWEPIYEVTQVKGDGEAHPTLSPDDEFADYENWDWDNIGRSQAKEDWMLPHEYARG
                     ALKLGLKHEKKLGVNPFKFGLIGSTDGHNTISTTEEDNFFGKFPESEPGPERLENAMA
                     MDRLWKNWNIVASGYAAVWARENTREALFEAMQRREVYATTGSRISVRFFGGWDYGAE
                     DIHRPTYLDVAYAGGVPMGGDLTAAPKDRAPKFMVHAVKDPDGANLDRVQVVKGWLER
                     DGSLQEKVYDVAWSGDRSPDPKTGKLFPVGSTVDVEAATYDNSIGTAQLSTVWEDPDF
                     DAKERAFYYVRVLEIPRPRWTAYDAAFFDLDLPEEIPMVIQDRAYTSPIWYTP"
     gene            complement(2121..3101)
                     /locus_tag="AC16PP_12495"
     CDS             complement(2121..3101)
                     /locus_tag="AC16PP_12495"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR6482493.1"
                     /translation="MEDPRLAGIGNSSINLKELRHVLLLHETLSFKRAAELAGVTQSA
                     LSQSIATLEERLGVKLFDRNRRKVSATGFGDLIAGRAVAILNDLSDMDQQIGAMRDVR
                     EGTMRFGMGIVPAVLILEQATSAFQAEHPDVATRVIVDVPEVLAERMEANEIEFFVAH
                     YPPQIRSRDHQHETLLSYEHRLICAPEHPLAGLNRVTFADLIRHPVVSFGPMYLADLG
                     LRTLRDSDDYRRFERNYPAVQTQQPWLLADLVAAGDFVMFAPGEPLADRIGQGLLVTR
                     EVADLQTRIEVKLVWKAGVSRSPAAKRMAEILHELAERQARDDLRRNFVE"
     gene            3312..>4509
                     /locus_tag="AC16PP_12500"
     CDS             3312..>4509
                     /locus_tag="AC16PP_12500"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6482494.1"
                     /translation="MSIKPLHGGPSRLLTLVCLLLPATPGFVVAEERTAAIEEIFVTA
                     RKREEPLQQTPVAVTALTGESLERVFATDITDLHVRAPNVIVSDISGFSSSAAVFIRG
                     IGNADIDSTVDPPVAIFIDGIYIPRPANNSLDLFDVEQIEILRGPQGTLFGRNTTGGA
                     IHYRTKRPTGEFGARGSVTVGEYGRRDIRMAAEAPIVEDKLAAKIAIFSQEYDGYFKN
                     TFTGTSGNRATKDQGGTDSLTIRPTILFTPNDTFDLTIIGEYIRERSEPTAQVPVGEP
                     TQLVPLRYGEPQYERGEDVRTVSYNVPGYINNDIWGITVEANWDVWGGTLTSVSNYRE
                     TDSLMNGEVDNSAAPAFEILRDEPHDQISTELRFAKDLTDNLNLITGLYYFEQDYFLR
                     RDTFIDV"
BASE COUNT          872 a         1475 c         1297 g          865 t
ORIGIN      
        1 atcgtcatgc tcgcttgggt ctaccattga tactcgtcgt gccaagatct cgtggttcgt
       61 cattctcaac cctccttcaa cagcgaaggc gacagctgga cggttgaagc ctacgttgcc
      121 aacctgaccg acgaggaaaa cgagcaggcc atcatcatca cgcaattcga caacacgcgg
      181 ttcttccagc gcccccgtac ctacgggctg cgattccgct atcgcatgtg atgtttcggg
      241 gccgcggcgc ccgccgcggc cctccccttc agggcgtgta ccagatcgga gaggtgtaag
      301 cccgatcctg aatgaccatg gggatctctt cgggcaggtc caggtcgaag aaggcggcgt
      361 cgtaagccgt ccaccggggc cggggaattt ccaggacccg aacgtagtag aaggcccgct
      421 ccttcgcatc gaagtccggg tcctcccaca ccgtgctcag ctgtgccgtg ccgatggagt
      481 tgtcgtaggt ggcggcctcg acgtccaccg tggagcccac cgggaacagc ttgcccgtct
      541 tcggatccgg cgaccggtca cccgaccagg caacatcgta gaccttctcc tgcaacgatc
      601 cgtccctttc gagccacccc ttcacgacct gaacccggtc gaggttcgcg ccgtccgggt
      661 ccttcacggc gtgcaccatg aacttcggag cacgatcctt gggcgccgcc gtcaggtcgc
      721 cacccatggg cacgccgccg gcgtaggcaa cgtccagata agtcgggcgg tgaatgtcct
      781 cggccccgta gtcccagccg ccgaagaagc gcaccgagat ccgcgagccg gtggtcgcgt
      841 agacctcccg ccgctgcatg gcctcgaaca gcgcttcccg ggtattttcg cgcgcccaca
      901 cggccgcgta gccggaagcc acgatgttcc agttcttcca gagccgatcc atggccatgg
      961 cgttctccag ccgctcaggg ccgggctcgg actcaggaaa cttgccgaag aagttgtctt
     1021 cctcggtcgt ggagatcgtg ttgtggccgt cggtgctgcc gatcaggccg aacttgaacg
     1081 gattcacgcc cagcttcttc tcgtgcttca gccccagctt gagggcgcct cgggcgtact
     1141 cgtgcggcag catccagtcc tccttggcct ggctgcggcc gatgttgtcc cagtcccagt
     1201 tctcgtagtc ggcgaactcg tcatccggag agagggttgg atgcgcctct ccatcgccct
     1261 tcacctgggt cacctcgtag atgggctccc agcgggcgcg cagctcggcg taggcgcggt
     1321 cgatgggttt gccgtccact gacttgtcag gaaacatcat gccgttggac aggttgccgt
     1381 tgtgggcgat ggccatcacc tcgcctccgg tctgggcctc gtaacgggca agcgccttcc
     1441 aaagctcccg gggatcgagg ctgtcctgcc ccgagaacgg gggcagctga gagaccttgt
     1501 ccgctgaatc cttgtaaacc accacccggt gcaggttgtt gccgtcgatc atcgccgtcc
     1561 actcgtagcc ggtgaaggcg gtgaactgcc ccggtcggtt gtactcgtcg gcggtcgtgg
     1621 ccacgtcctg ccagatcagc aggcgcgagc gctcgggcag gggcggatag ttctccggat
     1681 cctgcatgct gttgacgaag gtcgcgtaga tgtttgccac cgcatcgcca ccttcatcca
     1741 ggtagccctg ccattggcgg ccggcttcgg tctcggtgac gatgggatcg cccacgttga
     1801 agcggtaata accaccgatg tactcggagt ggtccgagac caccaggaaa tccagcgggc
     1861 gccgcagctg cacccgcatg ccgttgtgcg caacgacctc ctggccctgg gcaaaccgat
     1921 aggcgtctgc cggcgtgagg ttcatgttgc ccatggagta ggcgtccggc gagttgcgcg
     1981 tgtgcaggtg ggtgtctccc cagtacacgt tcttggggta atcgccgtcc accggaacgg
     2041 aatactcctg ggcccaaccc aaaccactcc agagcatcac ggctcccgcc agcggtcgaa
     2101 ccgccgctgc aataagatta ttactcaacg aagttcctcc tgagatcatc cctcgcctgc
     2161 ctctccgcaa gctcgtgtaa tatttccgcc attcgcttgg ccgccggcga gcgactcacg
     2221 cccgccttcc aaaccagctt cacctcgatg cgcgtctgga ggtcggcgac ctcgcgtgtg
     2281 accagaagac cctggccgat ccgatcggcc aacggctcac cgggggcaaa catcacgaag
     2341 tcccccgccg cgaccaggtc tgcaagcagc cacggttgct gagtctgcac cgcaggatag
     2401 ttgcgctcga accgcctgta gtcatcacta tcgcgcagcg tccgaaggcc cagatcggcg
     2461 agatacatcg gaccgaaact caccacgggg tggcggatca ggtcggcaaa cgtcacccga
     2521 ttcaggccgg ccaacgggtg ttcgggggca cagatcagac gatgttcata gctcaacaac
     2581 gtctcgtgct ggtggtcgcg gctgcgaatc tgagggggat agtgggcgac gaaaaattct
     2641 atctcgttcg cctccattcg ctctgccagc acctcgggca cgtcgacgat gacccgtgtc
     2701 gccacgtccg ggtgctcggc ctggaaagcc gacgtcgcct gttccagtat caatacggcc
     2761 ggcacgatgc ccatcccgaa acgcatggtg ccttcccgga catcccgcat cgcgccaatc
     2821 tgctgatcca tgtccgacag atcattcagg atcgccaccg cacgcccagc gatcaggtcc
     2881 ccaaatccgg tcgctgagac ctttctgcga ttccggtcga acagcttgac gcccaggcgc
     2941 tcctccagcg tcgcgatgga ctggctcagc gccgattggg tcacgccggc cagctctgct
     3001 gcacgtttaa agctcagagt ttcgtgaagc agcaggacgt gccgcagctc ctttaggttg
     3061 atggacgagt ttccaatccc ggctaggcga ggatcttcca tatgactctg ctgaaggtgt
     3121 gtcaaactca atattagctc aacttatgaa atatattaaa atttattgtt ttaattataa
     3181 ctatctgttt ttattaatga attttcttga agtctactcg gaacgagtta acatgcccag
     3241 ccgaaatgcg ccaaagatct cctgctgaac tcgttccgca gtccaacatg aaaaaccgag
     3301 gggattacca catgtcgatc aaaccacttc acggtggacc ttcgcgcctg ttgacgctgg
     3361 tgtgcctgtt gctgccggcc acacccggct ttgtcgtcgc cgaagagcgc accgccgcca
     3421 tcgaagaaat cttcgtcacc gcgcgaaagc gcgaggagcc gctgcagcag acaccggtgg
     3481 cggtaaccgc gctcaccggg gaaagtctgg agcgagtgtt cgccacggac atcaccgacc
     3541 tccacgtcag agcacccaac gtgatcgtgt ccgacatcag cgggttctcc agttcggcgg
     3601 ccgtcttcat ccgcggcatc ggcaacgccg acatcgactc gaccgtggat ccgccggttg
     3661 ccatcttcat cgacggcatc tacatcccac ggcccgccaa caactcactg gatctgttcg
     3721 acgtggagca gatcgaaatc ctgcgcggcc cccagggcac gctgttcggg cgcaacacga
     3781 caggcggggc cattcactac cgcaccaaac gacccacggg tgagttcggc gcacgaggca
     3841 gcgtcaccgt tggcgagtac gggcgccgcg acattcgcat ggccgccgag gcgcccatcg
     3901 tggaagacaa gctggcagca aagattgcca tcttctccca ggagtacgac ggctacttca
     3961 agaacacgtt caccggcact tcaggaaatc gcgccacgaa ggatcagggg ggcacagatt
     4021 cccttacgat tcgacccacc atcctcttca ccccgaacga taccttcgac ctcacgatca
     4081 tcggggagta catcagggag cgaagcgagc cgacagccca ggttccggtg ggggagccca
     4141 cccagctggt cccgctccgc tacggcgagc cccagtacga gcgaggcgag gacgtgcgca
     4201 ctgtcagcta caacgtgccc ggctatatca acaacgacat ctggggcata accgtcgagg
     4261 cgaactggga cgtgtgggga ggcacgctca cgtcggtttc gaactaccgg gaaacagatt
     4321 cgctcatgaa cggcgaagtg gacaacagcg ccgcgcccgc cttcgagatc ttgcgtgacg
     4381 agccccacga ccagatctcc accgagcttc gcttcgcgaa agatctaacc gataacctca
     4441 acctcatcac cggcctttat tacttcgagc aggattactt cctgcgccgg gacacgttca
     4501 tcgacgtgg
//
LOCUS       DBNHKV010000390         8784 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1117998, whole genome shotgun sequence.
ACCESSION   DBNHKV010000390 DBNHKV010000000
VERSION     DBNHKV010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8784)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8784
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1117998"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="AC16PP_12505"
     CDS             complement(<1..465)
                     /locus_tag="AC16PP_12505"
                     /inference="COORDINATES: protein motif:HMM:NF015662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR6482495.1"
                     /translation="MKGWRHYTLVGGFALGCVAVIVRLGYLNWTEREFLQEQGDARSV
                     RSEVLPAYRGVVYDRHGEPLAVSTPVFAVWLDPSRAGLTDESLGALAELLELDPARLS
                     ASVQSNGHREFLYLKRRVPWDRAQAVRELGISGVHLQSEYRRYYPASETTSHV"
     gene            complement(462..728)
                     /gene="ftsL"
                     /locus_tag="AC16PP_12510"
     CDS             complement(462..728)
                     /gene="ftsL"
                     /locus_tag="AC16PP_12510"
                     /inference="COORDINATES: protein motif:HMM:NF016860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HIR6482496.1"
                     /translation="MSRLYGWIVATLLVAVTVSGVQVAFSSQKVRQLHADLQAAQRVQ
                     DEELAEHSRLLLERSAQTSYVNVERVAEAELGMIFPDRVEPIEP"
     gene            complement(725..1657)
                     /gene="rsmH"
                     /locus_tag="AC16PP_12515"
     CDS             complement(725..1657)
                     /gene="rsmH"
                     /locus_tag="AC16PP_12515"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR6482497.1"
                     /translation="MSNTHEPVLCQETVATLTGMGNSTVRQGVYVDATFGRGGHSRAL
                     LAVLGDESRVVALDRDPAAVAAGREMANSEPRLTICHARFSDLRRALASLDIESVQGV
                     LMDLGVSSPQLDDPQRGFSFLRSGPLDMRMDPGAGMSAAQWLNSASEQEIAGVLRSLG
                     EERYARRIAAAIAAARPISDTGELVALVRAAQPRATPGKHEATRVFQAVRMHVNDELG
                     ELESGLRQAFEMLSAGGRLAVIGFHSLEDRMVKRFFKSLTEPPPLPRRLPVRAAAQVV
                     PARSVAGPVRAGAAERARNPRSRSAVLRVVEKIS"
     gene            complement(1654..2112)
                     /gene="mraZ"
                     /locus_tag="AC16PP_12520"
     CDS             complement(1654..2112)
                     /gene="mraZ"
                     /locus_tag="AC16PP_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR6482498.1"
                     /translation="MFRGINSATLDGKGRMALPTRHRESVVAVSEGRLVVTIDMREKC
                     LLMYPLPEWEVVQRKLESLSNIGEQARLLQRLLIGHATDLELDGQGRVLLPQMLRGYA
                     ALEKKLVLVGQGNKIEIWSDSLWQSRMNTWLSDGAGELLANGDEFTGLSV"
     gene            complement(2429..2807)
                     /gene="rnpB"
                     /locus_tag="AC16PP_12525"
     ncRNA           complement(2429..2807)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC16PP_12525"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2848..3666)
                     /gene="rsmI"
                     /locus_tag="AC16PP_12530"
     CDS             complement(2848..3666)
                     /gene="rsmI"
                     /locus_tag="AC16PP_12530"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR6482499.1"
                     /translation="MSGVLYVVATPIGNLDDITRRGADVLASVQIVAAEDTRRSQVLL
                     AAIGATQAELVSFHAHNEQNVARRLMAALQAGGDVALVSDAGTPLVSDPGFDLIRRCW
                     EEGVRVVPVPGASSVLAALSVCPLPAGRFFFEGFLPARAGQRRERLRHLAGRTEAVVF
                     FEAPHRVAACLADLEQLVPQRRLMVGREMTKRHESLHCGTPGELREALSSTLRGEFVF
                     VMEAAAGAGLDADAAEVMEILCAELAPAQAARLGSRLLKMDRSVLYRIALEQRS"
     gene            3761..5458
                     /locus_tag="AC16PP_12535"
     CDS             3761..5458
                     /locus_tag="AC16PP_12535"
                     /inference="COORDINATES: protein motif:HMM:NF016251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HIR6482500.1"
                     /translation="MPEVSPSSPEALLQQADLANGREAARLYLSATESFIQANRLDEA
                     ARSFQRIDQALLSGARTADYRYAAAEIALHDGEPDRAAALLGAGEPGGGSARRRGLWA
                     ATLPGRICHAAGDDQCAIRELTGLEVPGDQRQELHDVIWRYLGFAAGFSAPAQAGRTS
                     GREQGWWALKATVLQGISVEDQRQRVDDWRGAWPDHPAASVLPTALRAFDRDAPKVRR
                     VGLLLPLSGPLAVAGRAVRDGYIAAYLHESAQSEQEIIVYDTAAAPLPSLYEKALLDE
                     VDVLIGPLTKEEASALSGLAPSVPTLVLNYLEDGQRAPSNVYQLGLAIEDEADTMVSR
                     LLEDGAERLLVVHNDQDWSRRAARRLTSSWPYPVTREFFPDVKTITESVGRAMQIDAS
                     LERRDELASLLGTDLEFLPRARGDLDAVVALVDTVEANALVPALRFHFAQDLPVYASS
                     QTVRGATAQELGELAGFRVSELPWILFSDPIYLAMADVFLLQGNRFAALYALGVDAYR
                     VSGRLTLLASGGVHQLLGSTGLLTVDGSGRFRRELAWGLIRRGRLEPLPVMAGQSAL"
     gene            5455..5820
                     /locus_tag="AC16PP_12540"
     CDS             5455..5820
                     /locus_tag="AC16PP_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR6482501.1"
                     /translation="MTPQARAGAGAESAALDHLRARGLELVARNFRCRLGELDIVMVD
                     GNELVFVEVRLRNHARFGDGAASITAAKRQKLARAARAFLQRHPRYQSCPCRFDVVAI
                     KGEGQRMEMRWLKNAFAVG"
     gene            complement(5873..6265)
                     /locus_tag="AC16PP_12545"
     CDS             complement(5873..6265)
                     /locus_tag="AC16PP_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HIR6482502.1"
                     /translation="MKAKAKRPYLLRALYDWLVDSSLTPYVLVSAEEEAAMVPADYVN
                     DGKIVLNISPSAVRGLVIDDAGLSFDGRFGGAPYPVSAPISSVLAIYAKETGEGMIFD
                     PEEFPSRTDDSPEAPKDRPSGPELKVIK"
     gene            complement(6284..6916)
                     /locus_tag="AC16PP_12550"
     CDS             complement(6284..6916)
                     /locus_tag="AC16PP_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6482503.1"
                     /translation="MSVVTKRSSMTFFSDPRDHYCHRVRMVLAEKGVTVDIVDVDPSR
                     KPEDLAEINPYNTLPTLLDRDLVLYEANVIMEYLDERFPHPPLLPVYPVQRALSRLWI
                     TRVEKEWSGRLDILMAGKGRETTITKARKELRESIISIAPIFSEKPFFMNDEFTLVDC
                     CVAPILWRLKYVGINLPERSTRPLQRYMQVMFERSSFRESLSEAELEMRD"
     gene            complement(6971..7693)
                     /locus_tag="AC16PP_12555"
     CDS             complement(6971..7693)
                     /locus_tag="AC16PP_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HIR6482504.1"
                     /translation="MKSRSSFLWALTAATLTFSAGAFPAGSGWPLDSIDPDLEDLPSL
                     QNGFKLYVNYCIGCHSVKYQRYERTADDLGIPHDIAMDNLVLTGQKIGELMTTSMDPE
                     LAKNWFGAPPPDLTMVARVRGTDWLYTYLRTFYQDEARPFGVNNLVFENVGMPNVLAG
                     LQGEQVLDDSGSPELVAGTGQLSPEEFDQVIYDIVNFLYYTGDPSRLERHRTGIYVLL
                     FLVVLYVFTYLLGREYHKEIVD"
     gene            complement(7697..>8784)
                     /locus_tag="AC16PP_12560"
     CDS             complement(7697..>8784)
                     /locus_tag="AC16PP_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017132656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR6482505.1"
                     /translation="SGDGAFASVEYIMRDVEYGWLLRYLHTTGASAFFLVVYLHMFRA
                     MMYGSYRKPRELLWLIGMAIFACLIVEGFLGYLLPWGNMSYWAAQVIISLVGAIPVVG
                     PDLMEWVRGDFLVSDITLNRFFALHVVALPLVLVMLVFVHIVALHHVGSNNPDGIEIK
                     QNKDENGIPLDGVPFHPHYTIGHDLPAIVLFLFFFCIVVFYVPEMFGYFIEKPNFEEA
                     NSLKTPEHIAPVWYYTPFYAMLRAATFPLFGIPAKFWGFVVMAGAIVLPAVLPWLDRS
                     PVKSIRYKGWLSKGMLALFVISFFILGYLGTIAPSKPATALAQICTVVYFAYFVLMPW
                     YTRVEKTKPVPDRVTTKATRAAKAEGA"
BASE COUNT         1633 a         2897 c         2765 g         1489 t
ORIGIN      
        1 cacgtgagac gtcgtctcgc tggccgggta gtagcggcga tactcggact gcaggtgaac
       61 gcccgagatg cccagctccc tgacggcctg cgcccggtcc caggggaccc ggcgcttgag
      121 gtagagaaac tcccgatggc cgttgctctg cacggacgcg gacaggcgag cggggtccag
      181 ttcgagcaac tcggcaagcg cgcccagcga ctcgtccgtc aggccagcac ggcttggatc
      241 caaccagacc gcgaaaacgg gggtgctgac tgccagcggc tcgccatggc ggtcgtagac
      301 gacaccgcga taggccggca gcacctcgct gcgaacggat ctggcatcgc cctgctcctg
      361 caggaactcc cgttcggtcc agttgaggta accgagccga acgatcacgg ccacgcagcc
      421 cagagcgaag cctcccacca gcgtgtagtg cctccagccc ttcatggctc gatgggctcc
      481 acccggtcgg ggaagatcat gccaagctcg gcttccgcca cccgttcgac gttcacgtat
      541 gaggtctgcg cgctgcgctc caggagcagc cggctgtgct cggcgagttc ctcgtcctga
      601 acacgctgcg cggcctgaag atcggcgtga agctgccgca ctttctggct gctgaacgca
      661 acctgcacgc cggacaccgt gaccgcgacc agcagggttg ccacgatcca gccgtaaagc
      721 cgactcatga gattttctcc accacccgaa ggacggcgct gcgcgagcgc gggttgcgcg
      781 cccgttcggc agcgccagcg cggacgggcc ctgccaccga gcgcgccggg acgacctgag
      841 ccgccgccct taccggtaac cggcgcggca acggcggggg ctccgttaag ctcttgaaga
      901 agcgcttgac catccggtct tccagagagt gaaagccgat caccgccagg cgccccccgg
      961 cagatagcat ctcgaatgcc tgtcgcaacc cgctctcgag ctcgccaagc tcatcgttga
     1021 cgtgcatccg caccgcctgg aacacgcggg tggcctcatg cttgccgggt gtggctcgcg
     1081 gctgcgccgc gcgcaccagc gccaccagct caccggtatc ggagattggc cgggccgccg
     1141 cgatggcggc ggcgatgcgc ctggcatagc gctcctctcc gagcgatcgc agcacgccgg
     1201 cgatctcctg ctccgaagcc gaattgagcc actgcgcggc agacattccc gcgcccgggt
     1261 ccatgcgcat gtccaggggc ccggaccgca gaaagctgaa acctcgctga ggatcatcca
     1321 gctggggaga ggaaacgccg agatccatga gcacgccctg caccgactcg atgtcgagcg
     1381 aggcaagcgc ccggcgcaga tcagaaaagc gcgcgtgaca aatggtcaaa cggggctcgg
     1441 agttggccat ctcccttccc gccgcgacgg ccgccgggtc ccgatccagg gcaaccaccc
     1501 ggctctcgtc accaagaacc gccaggagcg cgcgcgaatg gccaccgcgc ccgaacgtgg
     1561 cgtcgacgta cacgccctgc ctgactgtcg agttccccat ccccgtcaat gtcgccaccg
     1621 tctcctggca aagcaccggc tcgtgcgtat tgctcaaacc gacaatccgg tgaactcgtc
     1681 gccgttggcc agcagttccc cagccccatc cgagagccag gtgttcatcc ggctctgcca
     1741 cagcgaatcg ctccaaattt ctatcttgtt gccctgaccg accagcacca gcttcttttc
     1801 caatgccgca tagcctctga gcatctgcgg cagcagcacc cgaccctggc cgtccagctc
     1861 gaggtccgtg gcatggccga tcagcagccg ctgcagcaac cgggcctgct caccgatgtt
     1921 cgaaaggctt tcgagcttgc gctgcaccac ctcccattcc ggaagcgggt acatcagcag
     1981 gcacttttcc cgcatgtcga tggtcacgac caggcgcccc tcgctcacgg cgaccaccga
     2041 ctcgcgatga cgggtcggca gagccatgcg ccccttgcca tccagagtgg cgctgttgat
     2101 gcctcgaaac atgactgccc tgggggccgc gcgaatcggt caaaatgaac caggtcacga
     2161 ccgattcaga cggaatctcc cacaatctcc cactaaatcc caaattggca caccaccagc
     2221 cacgatatcg acccacaggc ttgcctgtca agcgaggaaa acgtcgataa cctacggaaa
     2281 tttatggatt ttttcggcgc agagaggctg tcagaaggca gcgggcagct ttatcttctt
     2341 agttaacctc aggtagaagc aggtatctag aattcacctt aacttctaga cccaatcggc
     2401 attaaaaatc tcgttttagg tgcgaaaaga aacgagtcgg ccgataagcc gggttctgtt
     2461 gccgcgtccg agaacgcagc cgacagtcat tcatctaggg cgttcgtcac cgaacgcctc
     2521 gtgcgaccta cccgaacccg gtgcggacca caccatgggg ttcctatttg gccttgctcc
     2581 aggcggggtt tacctagccg cgcagtgtta ccacgcgcgc ggtgcgctct taccgcaccc
     2641 tttcaccctt accgttcgca gcgccggagc gccgcgcctt aggcggtttg ctttctgctg
     2701 cactttccgt gggctcgcgc cccccaggcg ttacctggca cctcgtcctg tggagcccgg
     2761 actttcctct cgcagctgat gctgccagcg actgtctggc cgactcgagg gcaagactaa
     2821 tccccggacc ggcggattgc caggccctca ggagcgctgc tccagggcga tccggtagag
     2881 cacggagcgg tccatcttga gcaggcgcga gccgagccgt gcggcttgag ccggagccag
     2941 ctcggcacaa aggatctcca tgacctcggc ggcgtccgcg tcgaggccag ctcccgcggc
     3001 ggcctccatc acgaagacga actccccccg aagggtgctc gacagcgcct cgcgcagctc
     3061 tcccggggtg ccgcagtgca gactctcgtg acgtttggtc atttcccgcc cgaccatgag
     3121 tcgccgctgc gggaccagct gctccagatc cgcaaggcag gcggcgaccc ggtggggcgc
     3181 ctcgaagaag acgacggctt ccgtgcgtcc ggcaagatgc cgcagccgct cgcggcgctg
     3241 ccccgccctt gccggcagaa agccctcgaa gaaaaagcgg ccggccggaa gaggacagac
     3301 ggacagagca gccaggaccg agctcgcgcc ggggacggga accacccgga cgccttcctc
     3361 ccagcagcgc cggatgagat cgaagcccgg gtcggatacc aggggcgtgc cggcgtcgga
     3421 gaccagtgcc acatcgccac ccgcctgcag cgcggccatg agacgccgcg ccacgttctg
     3481 ctcgttgtgc gcgtggaacg agaccagctc ggcctgggtt gcgccgattg ctgctaacag
     3541 cacctgagag cgtctggtat cctcggcggc gactatctgc accgacgcca ggacatcggc
     3601 gcctcgacga gtgatgtcgt cgaggtttcc aattggcgtg gcaacgacgt agagaacacc
     3661 agacatggca gcaaaccgga taagggcctg ggcttgggcg ttcgctattc tcgcagtgtc
     3721 cgcctgtcaa agcacgccgc tcgagacaat cgaagcgaac gtgcccgaag tctccccctc
     3781 atctcccgaa gctctcctgc aacaggcgga tctggccaac gggcgggaag cagcccggct
     3841 gtacctgtcg gccaccgaga gcttcattca ggccaaccgg ctcgacgagg ctgcccgatc
     3901 tttccagagg atcgaccagg cgttgctctc cggcgcccgg acggccgact atcgatatgc
     3961 cgccgccgag atcgcgctgc acgacggcga gccggatcgg gcggccgcgc tgcttggcgc
     4021 cggggagccg ggcggcggct cggcgcgacg gcgcggtctg tgggcggcca ccctgcccgg
     4081 ccgcatctgc cacgccgccg gcgatgacca atgcgcgatc cgggagctga ccggactcga
     4141 ggtacccggc gaccagcgcc aggagctgca cgacgtcatc tggcgctatc tgggttttgc
     4201 agccggtttc tccgcgcccg ctcaggccgg ccgcacctcc gggcgcgagc agggttggtg
     4261 ggctctcaag gccacggtgc tgcagggcat ctccgtggaa gaccagcgtc agcgtgtgga
     4321 cgattggcgc ggcgcctggc ccgatcaccc ggcagcttcc gtcttgccca cggctctccg
     4381 cgccttcgac cgggacgccc cgaaggtgcg acgggtcggg ttgctgctgc cgctcagcgg
     4441 ccccctcgcc gttgccgggc gcgccgttcg ggacggctac atcgccgcct atctgcacga
     4501 gtctgcccag agcgagcagg agatcatcgt ctacgacacg gccgccgcac cgctgccgag
     4561 cttgtacgag aaggccctcc tggacgaggt ggacgtgctc atagggcccc tgacgaagga
     4621 agaggcgtcc gccctcagcg ggctcgcccc gtccgttccc accctggtgc tcaactatct
     4681 cgaagacggc cagagggcgc cctcgaacgt ctaccagctc gggcttgcca tcgaggacga
     4741 ggccgacacc atggtgagca ggctcctgga ggatggcgca gagcgcttgc tggtcgtcca
     4801 caacgatcag gattggtcgc gtcgagcggc gcggcgactt acttccagct ggccctatcc
     4861 ggtcacgcgc gagttctttc cggacgtcaa aaccatcacc gaatccgtcg gacgggccat
     4921 gcagatcgac gcgagcctcg agcgccgcga cgagctggcc agtctcctgg ggaccgacct
     4981 ggagtttctg ccacgcgcca ggggggacct cgacgccgtg gtcgcgctcg tcgataccgt
     5041 cgaggccaat gccctcgtgc ctgcgttgcg ctttcatttt gcccaggatc tgcccgtgta
     5101 cgcgagctcg cagacggtgc gtggagcaac ggcacaggaa ctcggagagc ttgccgggtt
     5161 tcgcgtcagc gagctgccct ggatattgtt ctccgacccc atctacctgg ccatggccga
     5221 cgtgttcctc ctgcagggca atcgcttcgc cgccttgtac gcgcttggcg tggacgctta
     5281 tcgcgtgagc gggcgcctga cgctgctggc ctccggcggg gtccaccagc tgctgggcag
     5341 cacgggcctg ctcacggtgg atggctccgg acggttccgt cgggagctgg cgtggggact
     5401 gatccgcagg ggccgcctcg aaccgctccc ggtgatggcc gggcaatcgg ccctctgacg
     5461 ccccaggcac gggcaggtgc cggggcagaa tccgccgcgc tggaccatct ccgcgcgcgg
     5521 ggcctcgagc tggtagcccg caatttccgc tgccgtctgg gcgagctgga catcgtcatg
     5581 gtggacggca acgagctggt tttcgtcgaa gtccgtctgc gcaaccacgc tcgcttcggc
     5641 gacggcgcgg cgagcatcac cgccgcaaag cggcagaagc tggcaagggc cgcccgggca
     5701 tttctccaac gccacccccg ataccagagc tgcccttgcc ggttcgacgt ggtggccatc
     5761 aaaggagagg gtcaacgcat ggagatgcga tggctaaaaa acgcgttcgc ggtcgggtaa
     5821 tgattcgatc ctgccgccag ctggcgcggt gaagcaatga gaaatgcagc cgctacttga
     5881 tgaccttcag ctcggggccg gacgggcggt ccttcggcgc ttccggggaa tcatcggtac
     5941 gggaaggaaa ctcctccggg tcgaagatca tgccctcgcc ggtctccttg gcgtagatgg
     6001 ccagaacact cgagatgggc gcggacaccg gatagggcgc tccaccgaac cgcccgtcga
     6061 agctgagccc ggcgtcatcg atgacaagtc ctcgcacggc cgacggcgat atgttgagaa
     6121 cgatcttgcc gtcgttgacg tagtcggccg gcaccatcgc cgcttcctcc tccgcggaga
     6181 cgagcacgta cggggtgagc gaagagtcga cgagccagtc gtaaagggct cgaagcaggt
     6241 aaggacgttt cgccttggct ttcacgccag accgcccccg gggtcagtcc cgcatctcga
     6301 gctcggcctc ggagaggctt tctcggaacg agctgcgctc gaacatgacc tgcatgtagc
     6361 gctgcagggg acgcgtcgag cgttccggca ggttgatgcc gacgtacttc aatcgccaca
     6421 ggatgggcgc cacgcagcag tcgacaagcg tgaactcgtc gttcatgaaa aaaggcttct
     6481 cgctgaaaat cggcgcgatg ctgatgatgc tttcgcgcag ttccttgcgc gccttggtga
     6541 tggtcgtctc gcgacctttg ccggccatca gaatatccag ccggccgctc cactccttct
     6601 ccacacgcgt gatccaaagc cgcgacagag cgcgctgaac gggatagacg ggcagcagcg
     6661 gcgggtgcgg aaaccgctcg tccagatatt ccatgatcac gttggcctcg tagagcacga
     6721 ggtcgcggtc gagcagcgtc ggcaaggtgt tgtacggatt gatctcggcc aggtcctccg
     6781 gcttgcgcga cggatccacg tccacgatat ccacggtcac gcctttctcg gcgagcacca
     6841 tccgcacccg gtggcagtag tggtcccggg ggtcggagaa gaacgtcatt gaggaccgct
     6901 ttgtgactac gctcatgatt tacctttcgt ctgaaattcg cgttacaaga acctgccggc
     6961 ccccaacgat ctagtcgacg atttccttgt gatattcgcg gccgaggaga tacgtgaaca
     7021 cgtaaagcac taccagaaac agcagcacgt aaattcccgt acggtgacgt tccagacggc
     7081 tcggatcccc cgtgtagtag aggaagttca cgatgtcgta gatcacctga tcgaactcct
     7141 cgggcgagag ttgcccggtg cctgcaacca gctcgggtga accggaatca tccagcacct
     7201 gctctccctg gaggccggcc agaacgttgg gcatacccac gttctcgaac accaggttgt
     7261 tgactccgaa gggccgcgcc tcgtcctggt agaacgttcg caggtacgtg tacagccagt
     7321 ccgtgccgcg cacccgcgcg accatggtca gatccggggg aggcgcgccg aaccagttct
     7381 tggcgagctc cggatccatg ctggtggtca tcagctctcc gatcttctgg ccggtcagga
     7441 ccaggttgtc catcgcgatg tcgtgaggaa tccccagatc atccgccgtc cgctcgtagc
     7501 gctgatactt caccgagtgg cagccaatac agtagttgac gtaaagcttg aagccgttct
     7561 gcagggaagg caggtcctcc agatcaggat cgatgctgtc cagcggccag cccgagccgg
     7621 caggaaacgc gcctgcagag aatgtcagcg tcgcggcggt cagggcccag agaaacgagc
     7681 tgcgtgattt catgccttac gccccctcgg cctttgccgc tcgcgtcgcc ttggtagtca
     7741 ccctgtccgg caccggtttg gtcttctcca cccgcgtgta ccagggcatg agcacgaagt
     7801 acgcgaagta gacgacggtg cagatctggg cgagagccgt tgctggcttc gacggcgcga
     7861 tggttcccag gtatccgagt atgaagaagc tgatcacgaa cagcgccagc atccccttgg
     7921 acagccaccc cttgtagcgg atggatttca ccgggctgcg gtcgagccac ggaaggactg
     7981 cgggcagcac gatggcgccg gccatgacta cgaatcccca gaatttggcg ggaatcccga
     8041 agagggggaa cgtggccgcg cgcagcatcg cgtagaacgg cgtgtagtac cacaccggcg
     8101 cgatgtgctc gggtgtcttc agcgagttgg cctcctcgaa gttcggcttt tcgatgaagt
     8161 agccgaacat ctctggcacg tagaaaacca cgatgcagaa gaagaagaga aagagcacga
     8221 tggccggaag atcgtgtccg atggtgtagt gcggatggaa gggaaccccg tccaggggaa
     8281 tcccgttctc gtctttgttc tgcttgattt cgataccgtc ggggttgttg gagccgacgt
     8341 gatgcagagc gacgatgtgc acgaagacga gcatcaccag cacgagcggc agggccacca
     8401 cgtgaagggc gaaaaagcga ttcagcgtga tgtccgagac gagaaagtcg ccgcggaccc
     8461 actccatcag atcaggcccg accacgggaa tcgcgccgac tagcgagatg atcacctggg
     8521 cggcccagta ggacatgttg ccccagggca gcaggtagcc gagaaaccct tccacgatca
     8581 ggcaggcaaa tatcgccatg ccgatgagcc agagcagttc ccgcggcttg cgataggagc
     8641 cgtacatcat ggcccggaac atgtgcaggt aaacgaccag gaagaaggcc gaggccccgg
     8701 tggtgtgcag atagcgcagg agccagccgt actcgacgtc gcgcatgatg tactcgacgg
     8761 aagcgaaagc gccgtcgccg ctgg
//
LOCUS       DBNHKV010000391         6814 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1967042, whole genome shotgun sequence.
ACCESSION   DBNHKV010000391 DBNHKV010000000
VERSION     DBNHKV010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1967042"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /gene="leuB"
                     /locus_tag="AC16PP_12565"
     CDS             <1..1051
                     /gene="leuB"
                     /locus_tag="AC16PP_12565"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003116443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR6482506.1"
                     /translation="EIVPVAQAVMGAAALKFGVSVAFEEGLVGGIAIDETGSPLPDET
                     LRQAREARAVLLGAVGGPRWDDLPTLQRPERGLLGLRAGLELFANLRPAIQFGPLAEA
                     SSLRAELVAGLDILIIRELTGGIYFGEPRGIEVVDNERRGFNTYVYSEHEIERIARVA
                     FELARKRDRRVCSVDKANVLEVTQLWREVVSEVAGDYPDVELSHMYVDNAGMQLVRAP
                     KQFDVVVTGNMFGDILSDVAAMLTGSLGMLPSASLSSDGRGLYEPVHGTAPDIAGQDK
                     ANPLATILSVAMLFRYSLDAPEAARAIESAVDAVLAAGWRTADILQAEDSSKLRLIGT
                     REMGDRVLEQLETGA"
     gene            1074..2126
                     /locus_tag="AC16PP_12570"
     CDS             1074..2126
                     /locus_tag="AC16PP_12570"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF005957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR6482507.1"
                     /translation="MSSGVNVAIAGATGAVGEALLEVLEQRKFPVARLSLLASERSEG
                     KRLQFRGKSVPVERLDNFDFSDTAIAFFSAGGDLSAQFAPKAAAAGCIVVDNTSQFRY
                     DADIPLVVPEVNAHRLDQPNPRNIIANPNCSTIQMVVALKPLHDVARIRRINVATYQA
                     VSGAGASAIRELAGQTASLLNGKPIESKVFPVQIAFNALPHIDKFQDNGYTKEEMKMV
                     WETQKILEDEAIRVNPTCVRVPVFFGHSEAVHIETEHKITAEEARRLLAAAPGVTLID
                     DPSANGYPTAVSHGAGQDAVFVGRVREDVSFDRGLNLWVVSDNVRKGAALNSVQIAEE
                     LMARQPQLFAISDGLG"
     gene            2431..4662
                     /locus_tag="AC16PP_12575"
     CDS             2431..4662
                     /locus_tag="AC16PP_12575"
                     /inference="COORDINATES: protein motif:HMM:NF051932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="motility hub landmark protein FimV"
                     /protein_id="HIR6482508.1"
                     /translation="MIRSLLSRARAHVISIGAALSLLAAHAWGLGLGEIKLNSALNEK
                     LKAEIELLDASDMQPDEIRVSLASTEDFERVGVERFFFLTDLRFEVAFSSSSGVKVDV
                     TSTKPITEPYLNFIVEVLWPNGRLLKEYTLLLDPPTFSSAAAPVVAPPARTASSGGSG
                     DDVTRTAPSGGTRVSLAPPSPSTPAPRNIGGELMTDQSDTLWEIAQRTRPEGVSIAQD
                     MLAIQKLNPQAFIRNNINLLKAGYSLRLPTAEEARALSQQQAVALVAEQNADWRAYNR
                     GEAPAVETQVAQSVPSEFQGQVDATAERAEPVVESASEGELRIVAGVGDSVSGTAAAE
                     TVAQLDAAREEQDRLSREVDELAYQMDRERELSTNQLAVKDRQLEVKDQQIAELQAQM
                     AQVRQELNEARQNQNQSVPAPEQAKSWWESSYVLGGAAGALVLALVAGLIGARRKRPV
                     EDEVAVAAVTPRAARTSTTAAAAKAATADEGASVQAASGVSGDSGAHDAVPAPGDGDV
                     LAEAEIYIAYGRLPRAISLLQDAIEEEPERDDVRLKLLELYVESGERGAFSAQLTEFM
                     GRCQDEEMRDAARELEARLTGGALDVSKEAPVKAEAAEDEAESNVMDFDLGLDDEPAS
                     TTAVEADGGESEFDLELDEDEGASRGAGDQLGGDLGIDFKPEATALDADEEGSEVATI
                     DEDEFDFDVESDADSAETKLDLAKAYIDMGDEDGARDILKEVLEEGTSDQQTKAQTLL
                     EQL"
     gene            4662..5459
                     /gene="truA"
                     /locus_tag="AC16PP_12580"
     CDS             4662..5459
                     /gene="truA"
                     /locus_tag="AC16PP_12580"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR6482509.1"
                     /translation="MHLALGVEYDGSAFHGFQLQKAAATVQGALEQALSRIADHPLRI
                     AAAGRTDAGVHATGQVVGFASNAERPIQAWVKGVNALTPPSVKVRWAREVDPRFHPRY
                     SATARRYMYLWYEDDVTSPTLDRWAVRVRHLDDDAMHAASRSLVGEHDFSAFRASGCQ
                     SESAHRCVHRITVHRHGPLVVLDIAANAFLLHMVRNIAAALRDVGEGRRPAGWLAETL
                     AGRDRKHVGPTAPAHGLYLVGVRYPDQAFPPGEPPPLLRSLGGLARV"
     gene            5512..6147
                     /locus_tag="AC16PP_12585"
     CDS             5512..6147
                     /locus_tag="AC16PP_12585"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR6482510.1"
                     /translation="MPTKVKICGIARPEDARCVADAGADALGLIFVRQSPRFVSTGLA
                     REIAGEVAGSLSLIGLFVDADAAEVEAVLRDVDLDVLQFHGDEPGSYCEAFGVNYMKA
                     TRVSGPVDVAGLEAEYPGACCLLLDAYVPGVAGGTGVRLDLACWPEAGAKPLVLAGGL
                     TPENVADAISRVRPFGVDVSSGVEGGIKGRKDPERVRKFVSEVRRVGREGV"
     gene            6125..>6814
                     /locus_tag="AC16PP_12590"
     CDS             6125..>6814
                     /locus_tag="AC16PP_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR6482511.1"
                     /translation="MSEGKGFNKYDQPVGGHFGQHGGRFVAETLMHALDELEALYERA
                     RKDPEFQAELAHDLEHFVGRPTPLYHAARLSRDLGGAQIYLKREDLNHTGAHKINNTV
                     GQALLAKRMGKPRVIAETGAGQHGVATATVAARLGLECCVYMGAEDVKRQSLNVYRMK
                     LLGAEVVPVNSGSRTLKDALNEAMRDWVTNVDTTHYLIGTVAGPHPYPMLVRDFQAVI
                     GQEARAQSLSML"
BASE COUNT         1203 a         2136 c         2290 g         1185 t
ORIGIN      
        1 agagatcgtc ccggttgctc aggcagtgat gggcgcggcc gctttgaagt tcggcgtgag
       61 cgtcgctttc gaggaggggc tggtcggggg catcgctatc gacgagaccg gttctccgct
      121 gcctgacgag acgctgcgcc aggcccgcga ggccagggcc gtgctgctgg gggctgtcgg
      181 tgggcccagg tgggacgatc tgcctacctt gcagcggccg gaaagggggc tgctcggcct
      241 gcgcgccggg ctcgagcttt tcgccaacct gcgcccggcc atacagttcg gccctctcgc
      301 cgaggcgtcg tcgctgcggg ccgagctcgt tgccggcctg gacattctca tcatccggga
      361 gctcacaggc ggcatctatt tcggcgagcc gcggggcatc gaggtggtgg acaacgagcg
      421 tcgtggtttc aacacctacg tctactccga gcacgagatc gaacgaatcg cccgggtggc
      481 cttcgagctg gcgcgcaaac gggatcgcag agtctgctcg gtcgacaagg cgaacgtgct
      541 cgaggtcaca cagctctggc gggaggtcgt atccgaggtg gccggcgact atccggacgt
      601 ggagctcagc cacatgtacg tggacaacgc cggcatgcag ctcgtccgcg cgccgaagca
      661 gttcgacgtg gtggtgacgg gcaacatgtt cggcgacatc ctgtcagacg tggccgccat
      721 gctgaccggc tcgctgggca tgctgccttc cgcgtcgctg agcagcgacg gcagagggct
      781 ctacgagccc gtccatggca cggcgcccga catcgcgggg caggacaagg cgaatcccct
      841 ggccaccatt ctctcggtgg cgatgctctt ccgctacagc ctggacgcgc cggaagccgc
      901 ccgggccatt gagagcgccg tggatgcggt gctcgcggcc gggtggcgga cggccgacat
      961 tctgcaggcc gaggactcgt ccaagctccg gctcatcggt acccgggaga tgggtgacag
     1021 ggtgctcgag cagctcgaaa ccggggcgtg acaggcaatt ggaggcggat gccgtgagca
     1081 gtggagtcaa tgtggccatc gccggggcaa ccggcgccgt gggcgaggca ctgctcgaag
     1141 tgctggagca gcggaagttt cccgtcgccc gcctttcgct gcttgccagc gagcggtccg
     1201 aaggaaagcg cctgcagttt cgcggaaagt ccgtgcccgt ggagcgtctg gacaacttcg
     1261 atttttccga cacggccatc gcttttttct ccgccggcgg ggacctctcg gcgcagttcg
     1321 cgccgaaggc ggcggctgcc ggctgcatcg tcgtcgacaa tacctcccag ttccggtacg
     1381 acgcagacat cccgctggtc gttccggagg taaatgccca tcgtctggac cagccaaatc
     1441 cgcgcaatat cattgccaac ccgaactgct cgacgatcca gatggtggtg gcgctcaagc
     1501 ccctgcatga cgtggcccga atccgtcgca taaacgtcgc gacgtatcag gcggtgtccg
     1561 gtgctggggc gagcgccata cgggagcttg cgggtcagac ggccagtctg ctgaacggca
     1621 agcccatcga gagcaaggtc ttcccggtgc agattgcatt caacgcgctg ccacatatcg
     1681 acaagtttca ggacaacggc tacaccaaag aagagatgaa gatggtgtgg gaaacccaga
     1741 agatcctgga ggacgaagcg attcgggtga atccaacctg tgtcagggtg cccgtcttct
     1801 tcggccactc ggaagcggtg cacatcgaaa ccgagcacaa gatcacggcg gaggaagccc
     1861 gccgtctgct ggccgcagca cccggcgtca cgctgatcga tgatccctcg gcgaacggct
     1921 acccgacggc ggtgagccac ggcgcgggcc aggatgccgt gttcgtgggg agggtgcgcg
     1981 aggacgtctc cttcgatcgc ggtctgaatc tgtgggtggt ttcagacaac gtccgcaagg
     2041 gcgctgcgct caacagcgtg cagatcgcgg aggagctgat ggcgaggcag ccgcaactct
     2101 ttgccatatc cgatggtttg gggtaaaagg ctccagcaac ttaaccgtac cgacacatcc
     2161 ttcatgcccg ccagccgcgt cggagtcgtt tcctcagatc ccgcagcaag ctgcctgcag
     2221 aacggctttc ggggcgtccc gattcgggac tcagcgccca aaaccatcgc gaaactgtta
     2281 cctactgcaa tttttggccg tcccaagtga ggtatcgtct cgtttggcac atggatttga
     2341 tgaagtgcac aggatagcag tgagacattc actcggcgga acgggcgtcc gcatcaattt
     2401 cgctattact tttgttaaag gggctagcct atgatcagga gtctgctcag tcgcgcgcgc
     2461 gcgcacgtga tttctatcgg tgccgcgttg tctttgcttg cggcacacgc ctggggcctc
     2521 ggcctcggag agatcaaact gaactcggcg ctgaacgaga agctcaaggc ggagatcgag
     2581 ttgctcgacg cgtcagacat gcagcctgac gagatccggg tgtctctcgc ctctaccgag
     2641 gatttcgaac gggttggggt cgagcggttc ttcttcctga cggacctgcg cttcgaggtc
     2701 gcgttctcgt cctcgtccgg cgtcaaggtc gacgtcacga gcaccaagcc gataactgag
     2761 ccctacctca acttcatcgt ggaggttctc tggcctaacg gcaggctgct caaggagtac
     2821 acgctgctcc tcgatccgcc cacgttcagc agcgcggcgg ctccggtggt ggcgccgccc
     2881 gcacgcacgg cgtcctccgg cgggtccgga gacgacgtga cccgcacggc cccatccggc
     2941 ggtacccgcg tgtccctcgc gccgccgtcg ccctcgacgc ctgctccgcg gaacattggc
     3001 ggtgagctga tgaccgatca gagcgatacg ctctgggaaa tcgctcaacg cacccggccg
     3061 gaaggcgtgt ccatcgctca ggacatgctc gccatccaga agctgaaccc gcaggcgttc
     3121 attcgcaaca acatcaacct gctcaaggcc ggatactcgc tgaggctgcc cactgccgaa
     3181 gaggcgcgcg cgctgtctca gcagcaggcc gtcgccttgg tggccgagca gaacgctgac
     3241 tggcgcgcct acaatcgtgg cgaagcgccc gccgtggaga ctcaggtggc ccagtccgtg
     3301 cccagcgagt tccagggtca ggtcgacgcg accgcagagc gggccgagcc ggtcgtggaa
     3361 tccgcatcgg aaggagagct gcggatcgtc gccggggtcg gcgacagcgt atccgggacc
     3421 gccgctgcgg agacggtcgc gcaactcgat gcggcacgtg aagagcagga tcgcctttcc
     3481 agggaagtcg acgagctggc ctaccagatg gatcgggaac gtgagctttc cacgaaccag
     3541 ctggcggtaa aggatcgaca gctcgaggtc aaagaccagc agattgccga acttcaggct
     3601 cagatggccc aggttcgcca ggaactcaac gaggcgcggc agaaccaaaa ccaaagcgtg
     3661 cccgcgccgg aacaggccaa gagttggtgg gagtcgtcct acgttctcgg cggtgccgcg
     3721 ggcgctttgg ttttggctct cgttgccgga ttgatcggcg cgcgtcgcaa gcgccccgtc
     3781 gaggacgagg ttgctgtcgc cgccgtcacc cccagggcag cgcgtaccag caccacggct
     3841 gctgcagcca aggccgccac agccgacgag ggagcctcag tccaggcggc gtcgggcgtg
     3901 tccggggaca gcggagcgca tgacgcggtg cctgcaccag gtgacggcga tgtccttgcc
     3961 gaggccgaga tctacattgc ctacggtcgt ttaccgcggg ccatcagcct cctgcaggat
     4021 gctatcgaag aggagcctga gcgcgatgac gtgcgcctca agctgctcga gctctatgtc
     4081 gaatccggcg agcggggcgc tttttccgcc cagctgacag agttcatggg gcgctgccag
     4141 gacgaggaaa tgcgcgatgc cgctcgcgag ctcgaggctc gactgacggg tggcgcgctc
     4201 gatgtctcta aggaggctcc cgttaaggcg gaggctgctg aggacgaagc cgagtccaac
     4261 gtcatggact tcgatctcgg cctagacgat gagccggcct cgacgacggc ggtcgaagcg
     4321 gacggcggcg agagcgagtt cgatctggag ctcgatgagg acgagggcgc atcccgcggc
     4381 gccggcgatc agctcggcgg tgacctcgga atcgacttca agcccgaggc gaccgcgctg
     4441 gatgccgacg aagaaggaag cgaagtcgcc accatcgacg aggatgagtt cgatttcgac
     4501 gtcgaaagcg acgccgacag cgcggagacg aagctggatc tcgcgaaggc ctacatcgac
     4561 atgggtgacg aggacggcgc gcgggacatt ctcaaggaag ttctcgagga aggtaccagc
     4621 gaccagcaga caaaagcgca gacgctgctc gaacagctct gatgcacctg gcgctgggcg
     4681 tcgagtatga cggcagtgcc tttcacggct tccagctgca gaaggcggca gctacggttc
     4741 agggtgccct ggagcaggcg ctttcgcgta ttgccgatca tcctctgaga attgcagccg
     4801 ccgggcgcac cgatgccggg gttcacgcca cgggtcaggt cgtcggtttc gcctccaatg
     4861 ccgagcggcc gattcaggcc tgggtcaaag gggtgaacgc cctgaccccg ccatccgtca
     4921 aggtgcgctg ggcgcgggaa gtcgatcctc gattccatcc gcgctactcg gccacggcgc
     4981 gacgctacat gtatctgtgg tacgaggacg acgtgacctc cccgacgctg gatcggtggg
     5041 ccgtgcgggt gcggcacctc gatgacgacg ccatgcacgc ggcgtcgcgc agcctcgttg
     5101 gcgagcacga tttctcggcg ttcagagcaa gcgggtgcca gtccgagtcg gctcaccgat
     5161 gtgtgcatcg gatcaccgtc catcgtcacg gtcccctggt ggtgctggac atcgccgcaa
     5221 acgcgtttct tctgcacatg gtgcgcaaca tcgcggcggc tcttcgcgac gtcggcgaag
     5281 ggcgccggcc ggccgggtgg ttggccgaga cccttgccgg cagagaccga aagcatgtcg
     5341 ggccgacggc acctgcccat gggctctacc tcgttggcgt tcgctacccg gatcaagcct
     5401 ttcctcccgg cgaaccccca cccttgctgc gctcgctggg tgggctcgca cgcgtctaga
     5461 aggcccgtta cgtctccgct agactacgcc ccttttcccc cggatccggt cgtgccgacc
     5521 aaggtaaaaa tttgtgggat tgcgcggccc gaggacgcac gttgcgttgc ggacgccggc
     5581 gccgacgccc ttgggttgat cttcgtgcgg cagagccctc gcttcgtttc caccgggctc
     5641 gctcgtgaga tcgcagggga ggtggccggg tcgctcagcc tcatcgggct tttcgttgac
     5701 gcggatgctg ctgaggtgga agccgtcctt cgggacgttg acctggacgt gcttcagttt
     5761 cacggagacg agcccggatc ctattgcgag gcgttcgggg tgaattacat gaaggcaacg
     5821 cgtgtgagcg gccccgtgga cgtcgccggc ctcgaggccg agtatccggg cgcctgctgc
     5881 ctgcttctgg acgcctatgt cccgggggtt gccggcggca cgggcgtgcg cctggatcta
     5941 gcctgctggc cggaagctgg tgccaagccg ctggttctgg ccggtggcct gacaccggag
     6001 aacgtggccg atgccatcag ccgtgtgcgg ccgttcggtg tggacgtcag cagcggcgtc
     6061 gagggcggga tcaagggccg caaggacccg gagcgggtca gaaaatttgt cagcgaggtg
     6121 cgccgtgtcg gaagggaagg ggtttaacaa gtacgaccag cccgtgggcg gtcacttcgg
     6181 ccagcacggc ggccgcttcg tcgccgagac gctcatgcac gccctggacg agctggaggc
     6241 cctttacgag cgcgcacgca aggatcccga gtttcaggcc gagctcgcgc acgacctcga
     6301 gcacttcgtt ggacggccaa cccctctcta tcatgccgcg cgtctgtccc gggatctggg
     6361 tggcgcgcag atctacctca agcgcgagga cctcaaccat actggcgccc acaagatcaa
     6421 caacaccgtc ggccaggcgc ttctcgccaa gcgcatgggc aagcctcgcg tcatcgccga
     6481 gaccggcgct ggccagcacg gcgtcgcgac ggcgaccgtc gctgcccgtc tcggcctgga
     6541 atgctgcgtc tacatggggg ccgaagatgt caaaaggcag agcctgaacg tctatcggat
     6601 gaagctgctc ggggcggagg tggtccccgt caactccggc tcgcgcaccc tgaaggatgc
     6661 gctcaatgag gccatgcggg attgggtgac caacgtcgat acgacccact acctcatcgg
     6721 cacggtggcc ggaccccatc cctatcccat gctggtccgt gacttccagg cggtgatcgg
     6781 gcaggaagcg cgggcccagt ctctcagcat gctc
//
LOCUS       DBNHKV010000392         5152 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1473074, whole genome shotgun sequence.
ACCESSION   DBNHKV010000392 DBNHKV010000000
VERSION     DBNHKV010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1473074"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /locus_tag="AC16PP_12595"
     CDS             <1..1584
                     /locus_tag="AC16PP_12595"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR6482512.1"
                     /translation="REHPHPVRGGIPRQESQMQDIIEELQARRAAADLGGGEHRIEAQ
                     HGKGKLTARERISLLLDEGSFEEWDKFVEHRCTDFSMEEQKIPGDGVVTGYGTVNGRL
                     VFVFSQDFTVFGGALSEAHAEKICKVMDQAMKVGAPLIGLNDSGGARIQEGVASLGGY
                     AEIFQRNVLASGVVPQISLIMGPCAGGAVYSPAMTDFIFMVKDSSYMFVTGPEVVKTV
                     THEVVTPEELGGAKVHSGKSGVADLALENDVEALLATRRFVDFLPPNNREKPPRLTTD
                     DPANREEPSLDSLVPADPAKPYDIKEVIVKVADEADFFEIQPDFAKNIVVGFIRLQGS
                     TVGVVANQPMVLAGCLDIDSSKKGARFVRFCDAFNIPILTLVDVPGFMPGTAQEYGGI
                     IKQGAKLLYAYAECTVPKVTLITRKAYGGAYDVMASKHLRGDVNFAWPSAEIAVMGAK
                     GAVEIIFRKDLGDPEKIQARTDEYQQKFANPFVAASRGYVDDVFLPRETRQRICRSFA
                     MLRDKKLENPWRKHGNIPL"
     gene            1604..3589
                     /locus_tag="AC16PP_12600"
     CDS             1604..3589
                     /locus_tag="AC16PP_12600"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR6482513.1"
                     /translation="MFNKILIANRGEIACRVIRSARSMGIPTVAVYSDADARALHVTM
                     ADEAVRIGEAPSTESYLRMERIIEACRATGADAVHPGYGFLSENAQFREALDEAGIVF
                     IGPGTRAINAMGDKITSKKLAQEAGVNTIPGYTGVIDGPDHAVEIAREIGYPVMLKAS
                     AGGGGKGMRVVRDDDECRDGFERARNEARSSFGDDRVFAEKFIEDPRHIEIQVMADSH
                     GNVIYLGERECSIQRRHQKVIEEAPSPFLDEATRQAMGEQAVALAKAVDYESAGTVEF
                     IVDAERNFYFLEMNTRLQVEHPVTELVTGQDLVELMIKVAAGEPLPLSQADVTLKGWA
                     IETRVYAEDPFRNFLPSIGRLVHYRQPIETSSVRVDTGIVEGSEVSMFYDPMIAKLIT
                     YGEDRDQAIDRMADALDAYYIRGVSHNISFLNALISHPRFREGRLTTNFIAEEYPDGF
                     HATDLPPTDAMLPIAIVAAVHRAHVERSANISGQTRRFERQIGDDWIVIANGTHYPVN
                     VTAVEHGYEVTYEDSIYRIKTDWRIGDPLVVAEVNGRLVTVQVDRTTAGYRVSQGGTE
                     LDVRVATPTAASLMTHMLEKQAPDLSKFLLSPMPGLLVRLRVAEGEEAKAGEELAVVE
                     AMKMENSLRAEQDVKIAKVLAAEGDSLEVDQPILEFE"
     gene            3703..3894
                     /locus_tag="AC16PP_12605"
     CDS             3703..3894
                     /locus_tag="AC16PP_12605"
                     /inference="COORDINATES: protein motif:HMM:NF018058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HIR6482514.1"
                     /translation="MEKFRLGLSAAIAILLIILILQNMVTVEVRLLWWTVAMPRAILL
                     LLTAMAGFAVGILVRIRRR"
     gene            complement(3908..>5152)
                     /locus_tag="AC16PP_12610"
     CDS             complement(3908..>5152)
                     /locus_tag="AC16PP_12610"
                     /inference="COORDINATES: protein motif:HMM:NF016260.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HIR6482515.1"
                     /translation="LQADDVGPLRAFYQHVRELTGRLEGSVEFGGTLEEPRGAGSISL
                     RDGSLSLERPDLELRELQALLTLTEAGSVEVTGSASQADGRIELRGSGQDLFAAARAF
                     DVELTGSDLAVEHPDWTVRVSPDLRFTYTAGRGHLAGLIELPQAEIKVETLPAGAPRV
                     SDDVVVVGRDEQADTRRTRVTVDVTLVLGDQVALNAAGIAAQLEGRLNARVDAEGRQS
                     LHGALQIEGGAVLTQGQTLTIESGNVVYNGPVDNPYIDLRATRTIDTESPSVTVGVHI
                     RGSADNLTSTIFSDPAMSETRALSYLILGRDIGDPSDTDTSQLAAAAINLGLRSSSSI
                     VADLQRIAGLDELSAVAETQKSFAIVAGKRIGPSLYVRYTYNTLSTIGALIVRYDLTR
                     RWRVEALSSEYSAMDLFYRIGR"
BASE COUNT         1010 a         1647 c         1652 g          843 t
ORIGIN      
        1 cgggagcatc cgcacccggt ccgggggggc atcccacggc aggagagcca gatgcaggac
       61 atcatcgagg agctgcaggc gcgacgcgcg gcagccgatc tcggcggcgg cgagcaccgc
      121 atcgaagcac agcacggcaa gggcaagctc acagcccgcg aacgcatttc cctcctgctc
      181 gacgagggca gcttcgagga atgggacaag ttcgtggagc accgctgcac cgacttctcc
      241 atggaggagc agaagatccc cggagacggc gtcgtcaccg gctacggcac ggtgaacggg
      301 cggctggtgt tcgtcttcag ccaggacttc acagtgttcg gcggcgccct gtccgaagcc
      361 catgccgaga agatctgcaa ggtgatggac caggccatga aggtcggcgc gcctctgatt
      421 ggcctgaacg attccggcgg cgcaaggatc caggaagggg ttgcgtctct tggcggctac
      481 gccgagatct ttcagcgcaa cgtgctggcc tcgggcgtgg tgccgcagat ctcgctgatc
      541 atgggcccct gcgccggcgg cgccgtctac tctccggcca tgaccgactt catcttcatg
      601 gtcaaggaca gctcctacat gttcgtcacc ggtcccgagg tggtgaagac ggtcacccac
      661 gaggtggtca cccctgagga gctcgggggc gccaaggtgc acagcggcaa atcgggcgtt
      721 gccgacctgg ccctggagaa cgacgtcgaa gcgctgctcg ccacccgccg cttcgtcgac
      781 ttcctgccgc ccaacaaccg cgagaaacca ccacggctga cgacggacga tcccgcaaac
      841 cgtgaggagc cgtccctcga ctcgctggtg cccgcggatc cggcaaagcc gtacgacatc
      901 aaggaagtga tcgtgaaggt cgccgacgag gcggacttct tcgagattca gcccgatttc
      961 gccaagaaca tcgtcgtggg attcatccgt cttcagggat ccaccgtagg cgtcgtagcc
     1021 aatcagccca tggtgctcgc cggctgcctg gacatcgact cctcgaaaaa gggcgcgcga
     1081 ttcgtgcgct tctgcgacgc cttcaacatt ccgattctca ctctcgtcga cgtgccggga
     1141 ttcatgcccg gaacggccca ggaatacggg ggaatcatca agcagggagc caagctgctg
     1201 tacgcgtacg ccgagtgcac cgtgccaaag gtcacgctca tcacccgcaa ggcttacggc
     1261 ggcgcttacg acgtgatggc ctccaagcac ctgcgcggcg acgtgaactt cgcctggccg
     1321 agcgccgaga tcgcagtgat gggtgcgaaa ggcgccgttg agatcatttt ccgcaaggac
     1381 ctaggagacc ccgagaaaat ccaggcccga accgacgagt accagcagaa gttcgccaac
     1441 ccattcgtgg cggccagccg cggctacgtc gacgacgtct ttctgccccg ggagacccgc
     1501 cagcgcatct gccggtcgtt cgccatgctg cgcgacaaga agctggaaaa cccctggcgc
     1561 aagcacggca acattcccct gtagagcgga ggcggcgagc agcatgttca acaagatact
     1621 catcgcgaac cgcggggaaa tcgcatgccg ggtgatccgt tccgctcgca gcatggggat
     1681 acccaccgtc gccgtgtact cggacgcgga cgctcgggcg ctgcacgtca ccatggcaga
     1741 cgaggccgtg cgcatcggcg aggcaccttc cacggaaagc tatctgcgca tggagcggat
     1801 catcgaggcg tgcagggcaa ccggagccga cgccgttcat ccgggctacg gcttcctgtc
     1861 cgagaacgcc cagttccgcg aagcgctgga tgaggccggc atcgtcttca tcggtcccgg
     1921 cacacgggcc atcaacgcca tgggcgacaa gatcacgtcc aagaagctcg cccaggaagc
     1981 cggcgtgaac accattcccg ggtacacggg cgtgattgac ggcccggacc acgccgtgga
     2041 gatcgcgcgg gagatcggct acccggtcat gctgaaggcc agcgccggcg gcgggggcaa
     2101 gggcatgcgg gtcgttcgag acgacgacga gtgcagggac gggttcgaga gggcgcgcaa
     2161 cgaggcgcgc tcgagcttcg gcgacgaccg ggtgttcgcg gagaagttca tcgaggaccc
     2221 gcgccacatc gaaattcagg tcatggctga cagccacggc aacgtcatct acctgggaga
     2281 gcgcgagtgc tccatccagc gccgccatca gaaagtcatc gaggaggcgc cctctccgtt
     2341 cctcgacgag gcaacccgtc aggccatggg cgagcaggca gtcgctctcg cgaaagccgt
     2401 cgattacgag tccgccggca ccgtggagtt catcgttgac gccgagcgca acttctactt
     2461 tctcgagatg aacacccgcc tgcaggtgga gcatccggtc accgaactcg ttaccggcca
     2521 ggatctggtg gaactcatga tcaaagtggc cgccggcgag ccgctgccgc tgagccaggc
     2581 tgacgtgacg ctcaaaggct gggccatcga gacgcgggta tacgcggagg acccgttccg
     2641 caatttcctg ccttcgatcg gccgcctggt gcactatcgc cagcccatcg aaaccagcag
     2701 cgtgcgcgtg gataccggga tcgtggaagg cagtgaagtt tcgatgttct acgatcccat
     2761 gatcgccaag ctgatcactt acggcgagga tcgtgaccag gccatcgaca gaatggccga
     2821 cgcgttggac gcctattaca tccggggggt ctcccacaac atcagcttcc tgaacgcgct
     2881 catcagccac ccgcgcttcc gcgaggggag gctcactacc aacttcatcg ccgaggagta
     2941 ccccgacggc tttcacgcaa ccgacctgcc cccgacggac gccatgctgc cgattgccat
     3001 cgttgccgcc gtgcaccgcg ctcacgtgga acgttccgcc aatatcagcg gccagacacg
     3061 gcgcttcgaa cgacagatag gcgacgactg gatcgtgatt gccaacggta cgcactatcc
     3121 ggtgaacgtc acggcggtgg agcacggtta cgaggtgacg tacgaagact cgatctatcg
     3181 catcaagacg gattggcgca tcggagatcc cctggtggta gccgaggtca acggcaggct
     3241 ggtcacggtg caggtggacc ggacgacggc tggctatcgg gtttcccagg ggggcacgga
     3301 gctggacgtc cgcgtggcca cgccaaccgc ggcaagcctg atgacgcaca tgctcgaaaa
     3361 acaggcgcct gatctgagca agtttctgct ctctcccatg ccgggtctcc tggtccgact
     3421 cagggtcgcc gagggcgaag aggcaaaggc cggggaagag ctcgcggtgg tcgaagccat
     3481 gaagatggaa aacagcctgc gcgcagagca ggacgtcaag atcgccaagg tgctcgcggc
     3541 ggagggggac agcctggaag tcgaccaacc gattctcgag ttcgagtagg ccggctcacg
     3601 cccatccggg attaccgaaa accgtccaca gcagatcgca gcactgccca atgttgaagc
     3661 tcgcccttag aatgcgccct gactccacct tccggtaaga ccatggaaaa attcaggttg
     3721 ggcttgagtg ccgccatagc aattctgctg atcatcctga ttctgcagaa catggtgacc
     3781 gttgaggtcc gtctgctctg gtggacggtg gccatgccaa gggccattct gctgttgctg
     3841 accgccatgg ccgggttcgc ggtcggcatt ctcgttcgga tacgcagaag atgagaagct
     3901 cacggctcta gcggccgatc ctgtagaaaa gatccatggc ggagtactcg ctggaaagcg
     3961 cctccacgcg ccagcgcctg gtgagatcgt aacggacgat gagcgcgccg atggtcgaaa
     4021 gggtgttgta cgtatagcgg acgtacaggc tgggcccgat ccgttttccg gcaacgatgg
     4081 caaagctctt ctgggtctcg gcaaccgccg acagctcatc aaggcccgcg atgcgctgca
     4141 ggtcggcgac gatggacgac gaggaccgga gcccgaggtt gatcgctgcg gccgcaagct
     4201 ggctggtatc cgtatccgac gggtcgccga tgtcgcggcc caggatcaga taggaaagcg
     4261 cccgcgtctc ggacatcgcg gggtccgaga atatcgttga agtcagattg tcggcgctgc
     4321 cgcggatgtg cacgccaacc gtgacagacg gactctcggt gtcgatggtc cgggtagcgc
     4381 gcaggtcgat gtagggattg tccaccgggc cgttatagac gacgttgccg gactcgatgg
     4441 tcagcgtctg accctgggta agcacggccc cgccctcgat ctgcagggca ccgtgcaacg
     4501 attgccgccc ctcggcatcc acccgagcgt tgagacggcc ttccagctga gcggcgatcc
     4561 ccgctgcgtt gagcgctacc tggtctccca ggacgagcgt cacgtccacc gtcacgcggg
     4621 tgcggcgcgt gtcagcctgc tcgtcccgac caacgaccac cacgtcgtcg gaaacacgcg
     4681 gcgcgccggc gggaagggtc tctaccttga tctcggcctg gggcagctcg atgagccccg
     4741 caagatgtcc gcggccggcc gtgtaggtga accgcaggtc gggcgacacc cgcaccgtcc
     4801 agtcgggatg ctccacggcg aggtctgacc ccgtcagctc gacgtcgaac gcccgggcgg
     4861 cagcgaagag atcctgcccc gagcctcgca gctcgattcg gccatcggcc tgggaggcgc
     4921 tgcccgtcac ctcgacggat ccggcctcgg tcagcgtcaa caacgcttgc agttccctga
     4981 gctcgaggtc cggacgctcc aggctgagac ttccatcgcg caaagagatc gagcctgcac
     5041 cccgaggttc ttccagcgtt ccgccgaact ctaccgagcc ttcaagtcgc ccggtgagct
     5101 cgcggacatg ctggtagaag gcccgcaaag gcccgacgtc gtcggcctgc ag
//
LOCUS       DBNHKV010000393         1855 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_551694, whole genome shotgun sequence.
ACCESSION   DBNHKV010000393 DBNHKV010000000
VERSION     DBNHKV010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_551694"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..>1855
                     /gene="fusA"
                     /locus_tag="AC16PP_12615"
     CDS             286..>1855
                     /gene="fusA"
                     /locus_tag="AC16PP_12615"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR6482516.1"
                     /translation="MAYTTADIRNVCLLGPAEAGKTQLAEALLYAGGAIAQCGQVAKG
                     NTVTDHSERERAIGHSLYPAVCHTDHAGIHVNVVDTPGYRDLYGRALAVLPAVETAVI
                     VINAEKGIEEMTRRMMEAAQAQHLCRMIVVNKIDSAEADLAGLVGEIQEAFGGECLPI
                     NLPASHGDGVVDCYFHPSGGETVFGSVEQAHTRLIDQVVEVDDELMELYLEQGDDLAP
                     EQLHEPFERALREGHLIPVCFVSARTGVGVADLLNVFERLMPNPEEGNPPEFLKGEGA
                     EAQPVSVVPDPEQHVVAHVVQVDVDPFKGRLAVFRIHQGSIRPGMQLYVGDARKPIKV
                     NQLLKLNGNGHSKVEVGLPGDICAIPRVDEAHYDAVLHDSHDEDHFHLRSEPLPPPMF
                     GLAIQPGNDADAQKTSDALHIMEAEDPSLQIEHVAALNEIVLRGLGELHLRSVLEEIE
                     ARHDLRVETALPSIAYRETITKPAQGHCRHKKQTGGAGQFGEVFLRIEPLPPGGGFEF
                     VSEVVGGAIPSQFIP"
BASE COUNT          327 a          605 c          615 g          308 t
ORIGIN      
        1 cgccgagatt ccccgcaacc cgtcgggcaa gatcctcaag gtcgagcttc gcaagccgta
       61 ctgggaagac cgggaacgtt tcgtttctta agcccgcgcc caggtttcct cgggcccggc
      121 cccagcgtga ggcgggcgcg caccgtttcg gtgcgtgcgg gcggcgtttc cacctgggtg
      181 gcgtgctggg gcgctccgtc aaccccctgt tttctggcgg gtgtgacccg accgtttccg
      241 tgaggtaagc ttcgaaggtg agcttgaggt aggggggaag accacatggc ttacactact
      301 gcggacatcc gcaacgtctg cctgcttgga ccggccgagg ccggaaagac tcagctggcc
      361 gaagcgctgc tgtatgcagg gggcgccatc gcccaatgcg ggcaggtcgc caagggcaac
      421 accgttaccg accacagcga acgcgaacgc gccatcggcc actctctgta tcccgcggtg
      481 tgtcatacgg atcacgccgg cattcacgtg aacgtcgtgg acacgcccgg ctaccgggac
      541 ctttacggcc gagccctggc ggtgctcccg gcagtggaga ccgccgtcat cgtcatcaac
      601 gcggagaagg gcatcgagga gatgacccgg cgcatgatgg aggcggcaca agcccagcat
      661 ctgtgccgca tgatcgtcgt caacaagatc gacagcgccg aggccgatct tgccgggctc
      721 gtcggcgaga ttcaggaggc tttcggtggc gagtgcctgc ccatcaacct gcccgcctcc
      781 catggcgacg gtgtggtgga ctgctatttc catccctccg gcggggaaac cgtgttcgga
      841 agcgtggaac aagcgcacac ccggctgatc gatcaggtgg tggaggtgga cgacgagctc
      901 atggagctct acctggagca gggcgacgac ctcgcgccgg agcagctgca tgagcccttc
      961 gagcgggcgc tgcgggaagg tcacctgatt cccgtgtgct tcgtctccgc ccgcacgggc
     1021 gtgggcgtcg ccgatctgct gaacgtcttc gagcggctca tgccgaatcc ggaagagggt
     1081 aacccgcccg agttcctgaa gggcgagggt gccgaggccc agcccgtcag cgtggttccg
     1141 gatcccgagc agcatgtggt ggcccacgtc gtccaggtgg acgtcgaccc cttcaagggc
     1201 cgcctggcgg tgttccggat ccatcagggc agcatccgcc cgggcatgca gctctacgtc
     1261 ggcgacgccc gcaagcccat caaggtcaac cagctcttga agctcaacgg caacggccac
     1321 tccaaggtgg aggtgggtct gccgggggat atctgcgcca tcccgcgagt cgacgaagca
     1381 cactacgacg ccgttctgca cgactcccac gacgaggacc actttcacct gcgctccgag
     1441 ccgctgccgc cgcccatgtt cggtcttgcc atccagcccg ggaacgacgc cgacgcccag
     1501 aaaacgtcgg acgccctgca catcatggaa gcggaggatc cttcgctgca gatcgagcac
     1561 gtcgccgcgt tgaacgagat cgtgctgcgc gggctgggcg agctgcatct gcgctcggtg
     1621 ctcgaagaga tcgaagcgcg tcacgacctc agggtcgaaa cggcgctgcc ctccatcgcc
     1681 taccgggaga ccatcacgaa accggcgcaa ggccattgcc ggcacaaaaa gcagacgggt
     1741 ggcgcgggcc agttcggcga ggtgtttctg cggatcgagc cgctgccgcc gggcggaggt
     1801 ttcgagttcg tcagcgaggt cgtcggcgga gccatcccgt cacagttcat tcccg
//
LOCUS       DBNHKV010000394         7060 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_977929, whole genome shotgun sequence.
ACCESSION   DBNHKV010000394 DBNHKV010000000
VERSION     DBNHKV010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7060)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7060
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_977929"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="AC16PP_12620"
     CDS             <1..449
                     /locus_tag="AC16PP_12620"
                     /inference="COORDINATES: protein motif:HMM:NF019528.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase family protein"
                     /protein_id="HIR6482517.1"
                     /translation="AQPETNLSLCHMDLRLANVFLDEESDDPVVLYDWQSMYPGRTAQ
                     DLGYLITSGYTPAFRREHERNLVSRYHQRLVDAGIPGYSFDEAWFDYRHGIIIGLRLL
                     PMALGDLDVSTEGGQAIFRKIIRNFPEAALDHGGVELVDEILARRA"
     gene            complement(484..1122)
                     /locus_tag="AC16PP_12625"
     CDS             complement(484..1122)
                     /locus_tag="AC16PP_12625"
                     /inference="COORDINATES: protein motif:HMM:NF013695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation efflux transmembrane domain-containing
                     protein"
                     /protein_id="HIR6482518.1"
                     /translation="MSDDCCANGIDASGLETRHRRALASVLAINVAAFLVMIVGAVLS
                     GSSSLLSGTLDNLGDALTYALSLAVIGSTASAKARVAFFKGLLIFAAALGVAAQIGWR
                     LSNMETPVFETMGVAATLNLAANLICLRILFPFRNDDLNMTSVWECSRNDIFDGLAVL
                     TATVAVWVSDSAWPDLLIAIALLIVFLRSAARILSRSWRELHAAPTPGPGTG"
     gene            complement(1119..2108)
                     /locus_tag="AC16PP_12630"
     CDS             complement(1119..2108)
                     /locus_tag="AC16PP_12630"
                     /inference="COORDINATES: protein motif:HMM:NF021778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YVTN family beta-propeller repeat protein"
                     /protein_id="HIR6482519.1"
                     /translation="MTRSFHGLILVLIAAVSSAIEAAAEPTGTLLVVNKGGDSVTMLD
                     VSDGRELAELPTGRGPHELVVSPDAKLAVVTDYAGGNSLTVIDIAGRRVVRTVDLEDY
                     PRPHGIAFMPDGDTVVVTSEATDQIVFVRISDGAVVGSIETGGAGSHMLAITANGSRV
                     FTSNIQSDTVSELDVASGKQVRAYPTPKQPEAIAVTADGSEIWVGSNAEGSLSVIRTD
                     DATIETIAEGFGWPYRILISRDGRLVLVPDLKRNVLRMFDRTNRVELASVELADAAPQ
                     GLVFHPDERWLFVSLNGQNQVAVIDLSRSAIVGRIDTGPRPDGIGYAATKGQP"
     gene            complement(2149..2970)
                     /locus_tag="AC16PP_12635"
     CDS             complement(2149..2970)
                     /locus_tag="AC16PP_12635"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6482520.1"
                     /translation="MELIVFDLDGTLLNKSAEISDYTRDTLSMLADRGILYTVATGRT
                     LHASVEVLEGHGFRLPQAYKNGVMIWNPTLGDYTHSNFLTLDEIQHVLEAVMRQDITP
                     FMFTLEPGNVHAIYHPPLKTEIEERLAADFRSRSGVGVYPAAQMPPEAEITNISALGI
                     PQAIDAVDLMIADEPHLVAYAGTAIEGSELKWIDIHHVEASKGNAIEILRKELGATKV
                     LCFGDNNNDLSMFEVADESYAPSNAQPEILEAATAVIGHHDEDGIARFLRERFSL"
     gene            3112..4011
                     /locus_tag="AC16PP_12640"
     CDS             3112..4011
                     /locus_tag="AC16PP_12640"
                     /inference="COORDINATES: protein motif:HMM:NF006128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR6482521.1"
                     /translation="MSQKSYENIKYEVDNGRARISLNRPEKRNALSIELVEELRDALW
                     EADDDTAVHCVILKGEGPSFCAGYDLTAGRSKVDDGVKRRSGASFDDDAWRIERFQRS
                     LRTLAEMHKPSIAQIHGNCLAGGTDLALYCDILICADNANIGYSALRNMGAAPNQMWL
                     YHCGPQWTKRLLLTGDTLSGEDAARIGLVLKSVPEAYLESEVEGLADRLSWIDPEMLS
                     TNKRIINVGMELMGAQVLQRLAAENDARAHTTQAARNVFKQIATEGLRSALRDRDAPF
                     GDSRARVIGPETRDEQGRLVPWE"
     gene            complement(4042..4647)
                     /locus_tag="AC16PP_12645"
     CDS             complement(4042..4647)
                     /locus_tag="AC16PP_12645"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HIR6482522.1"
                     /translation="MTYLEWRPHPWHGLEPGDLDSGVVNAFIEITPFDTVKYEVDKES
                     GYLRVDRPSRTSSLPPTLYGFVPRTYCGDRVKVLMEGADSGDFDPLDICVISDRLVNR
                     AEVIIPARVVGGLPMLDGGEADDKIIAVHANDEIWRDTNDIGDLPEQYVARLRHYFAS
                     YKTLPGESSTVHVGEPYGRERALRVLHASISDYQEQFPANP"
     gene            4816..5820
                     /locus_tag="AC16PP_12650"
     CDS             4816..5820
                     /locus_tag="AC16PP_12650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6482523.1"
                     /translation="MSTSNTRVLLAKRPVGQATPDCFSVETEQLPALEPGQVRVAVEY
                     ISVDAGTRTMLRGEGFHHQVGLGQTILAGGVGHIVDSAADGWELGQAVRGGLGAQTIA
                     TVTPELIERVDESVAPLSAYLGVLGGSTGVTAWIGMRRVAKPQPGDVFVVSAAAGAVG
                     SIAGQIAKRDGARVVGIAGGPRKVSYLLERLGFDAAIDYKNEDVNARLRELAPDGVNV
                     FFDNVGGPILDAVLDNIAMRARVVICGAISQYDDLDNVTGPSLYLRLAERQSTMEGFA
                     FFHFPESIGPATEELARWLADGALVFDEEILEGIERYPEALGFMFEGGNIGKLLVKAR
                     "
     gene            5880..6497
                     /locus_tag="AC16PP_12655"
     CDS             5880..6497
                     /locus_tag="AC16PP_12655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6482524.1"
                     /translation="MSEDFSLREAIYNCRAMRRIRPDPVPEELLVELIDAANQAPSGS
                     NSQRARWIVVRDPAVKRQLADQNRRHAEPYIAADVSNPATPKLKRMLDAVVWQMEHMH
                     EIPALIVACFDYPEKVEGMGVYRSAGSVWPGIQNLLLTARALGLGAAPTTLALRDQDA
                     VRKILKLPETFAALCLIPVGYPMGKFGPVTRKPVSEVMRFDAWND"
     gene            6539..6916
                     /locus_tag="AC16PP_12660"
     CDS             6539..6916
                     /locus_tag="AC16PP_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HIR6482525.1"
                     /translation="MTRWIAVLLMLLWCRPALGGDIGGTWKNDADPVWIEVVHDDGVA
                     TGTVVRNDRNPAAAGRLLLKDLKPGDEANVWRGQVFAVRLGEFRDAQVRLTGDDRMEI
                     LVKVGFMSRTVTWTRADQAVGAE"
BASE COUNT         1253 a         2141 c         2370 g         1296 t
ORIGIN      
        1 ctgcgcaacc ggaaaccaat ctgtctctct gccacatgga tttacggctg gctaacgtgt
       61 tcctcgatga ggaatccgac gatccggtcg ttctctacga ttggcagtca atgtatcccg
      121 gcagaacggc ccaggatctg gggtatctga tcacctcggg gtacacgccg gcgtttcgtc
      181 gcgagcatga acgtaacctg gttagtcgtt accaccagcg gctggtggac gcggggatcc
      241 cgggttacag cttcgatgaa gcctggttcg actaccggca cggcatcatc atcggcctgc
      301 ggctgttgcc gatggcgtta ggcgatctgg acgtctccac cgagggcggc caggccatct
      361 tcaggaagat cattcggaac ttccccgagg cagccctgga ccacggcggc gtcgagctgg
      421 tggatgagat tctggcccgg cgagcataga tctgcctgac gctgccatcc ggtcgccgtt
      481 ttcctacccg gtccccggcc ccggcgtggg tgcggcgtgc agctcccgcc aggaacggct
      541 gagaatgcgg gcagcggatc gcaggaaaac gatcagcaga gcgatcgcga tcaagaggtc
      601 cggccatgcg gagtcggaaa cccacacggc cacggtggcg gtcagcactg caaggccgtc
      661 gaagatgtcg ttgcgcgagc actcccagac ggacgtcatg ttcaggtcgt cgttccggaa
      721 gggaaacagg atgcgcaggc agatcaggtt cgcggccagg ttgagggtgg ccgccacccc
      781 catggtttcg aacaccggcg tctccatgtt ggaaagccgc cagccgatct gcgcggccac
      841 cccgagggcg gctgcaaaga tcagcaagcc cttgaaaaag gcgacccggg cctttgcgga
      901 tgccgtggaa ccgatgactg ccaggctaag ggcgtaggtc agcgcgtcgc cgaggttgtc
      961 cagggtgccg gagagcagag aggatgagcc gctgagcacg gcgcccacga tcatcacgag
     1021 gaatgcggcg acgtttatgg cgagaacgga cgccagcgcg cgccggtgcc gcgtttcgag
     1081 accgctcgcg tcgattccgt tggcgcagca atcgtcgctc atggctgccc cttcgtcgcg
     1141 gcgtagccaa tgccgtcagg ccgtgggccc gtgtcaatgc gcccgacgat ggcggatctg
     1201 ctcagatcga tgacggccac ctgattctga ccattgagcg agacgaaaag ccagcgttca
     1261 tccggatgga agacgagtcc ctggggcgcc gcgtccgcca actccacgct ggctagctct
     1321 acccgattgg tgcgatcgaa catgcgcagc acgttcctct tgaggtccgg gaccagcacc
     1381 agcctgccgt ccctggagat caggatacgg tacggccaac cgaagccttc ggcaatcgtc
     1441 tcgatggtgg cgtcatcggt ccggatcacg ctcagcgagc cttcagcgtt gctgccgacc
     1501 cagatttccg agccgtcagc ggtcactgcg atggcttcgg gttgtttcgg ggttgggtag
     1561 gcgcgcacct gtttcccgga ggccacgtcg agctcggaga ccgtgtcgct ctggatgttg
     1621 ctcgtgaaga cgcgcgatcc gttcgcggtg atggcgagca tgtgggatcc ggcgccgccg
     1681 gtttcgatgg atcccaccac ggccccgtca gaaatgcgca cgaacacgat ctgatcggtg
     1741 gcttcggacg tcacgaccac cgtgtcaccg tccggcatga aggcgatacc gtggggccgc
     1801 gggtagtcct cgagatccac cgtgcggacg acgcgtcgcc cagcgatgtc gatgacggtg
     1861 agcgagttgc cgccggcgta gtccgtcacc acagccagct tcgcgtccgg cgaaaccacg
     1921 agctcgtgcg gtccccggcc tgtgggaagc tccgcgagct cgcggccatc ggacacatcc
     1981 agcatcgtta cgctgtcgcc tcctttgttg acgacgagga gcgttcccgt cggctcggcc
     2041 gccgcctcga tggctgaaga caccgcggcg atgagcacga ggatcaggcc gtggaaactt
     2101 ctggtcatcc tgtctccctg ctcatcgggg tgcggcgtcc gtcctctgct acagcgagaa
     2161 ccgctcgcgg agaaatcgcg cgatgccgtc ctcgtcgtga tggccgatca cggcggtcgc
     2221 cgcctccaga atctcgggct gggcgttgct gggcgcgtag ctttcgtctg cgacctcgaa
     2281 catgctgagg tcgttgttgt tgtcgccgaa gcacaacacc ttggtcgcgc cgagctcctt
     2341 gcgcaggatc tcgatggcgt tgcccttgct cgcctccacg tggtgaatgt cgatccactt
     2401 cagctccgag ccttcgattg ccgtacccgc atacgccacc agatgcggct cgtcggcaat
     2461 catcagatcg acggcgtcga tggcctgggg tatcccgagc gcgctgatgt tggtgatctc
     2521 ggcttcgggg ggcatctgag cggcgggata cacgcccacg ccggaccgtg aacggaaatc
     2581 ggcggccagg cgttcctcga tctcggtctt caggggcgga tggtagatcg catggacgtt
     2641 gccgggctcc agcgtgaaca tgaagggcgt gatgtcctgg cgcatcaccg cctccagaac
     2701 gtgctggatc tcgtcgaggg tcagaaagtt gctgtgggtg tagtccccaa gcgtcgggtt
     2761 ccagatcatc acaccgttct tgtacgcctg gggcaggcgg aagccgtggc cctcgaggac
     2821 ctcgacggac gcgtgcagcg ttcggccggt ggcgaccgtg tacaggatgc cgcggtccgc
     2881 cagcatcgaa agcgtgtcgc gggtgtaatc ggagatctcc gcggatttat tcagcagggt
     2941 gccgtcgagg tcgaaaacga tcagttccat ccggcgcagt gttgcagact gagcggctgg
     3001 gtcaaacctt tcgggattcg ccgtgctccg ggaggttcca acggttgctg ctacccgtgg
     3061 ggcgcaggca atatccggaa ctgccgctac cgcgcatcca caggagatcc catgagccag
     3121 aagtcatacg agaacatcaa gtacgaggtc gacaacggac gagcccggat ctctctgaat
     3181 cggccggaga agcgcaacgc gctgtccatc gagctggtcg aggagctcag agacgcgttg
     3241 tgggaagccg acgacgatac ggccgttcac tgcgtcattc tgaaagggga gggcccctcg
     3301 ttctgcgcgg gctacgacct gacagccggt cggtcgaagg tggatgacgg ggtcaagcgc
     3361 cgttctggcg ccagcttcga cgacgatgcg tggaggatag aacggtttca gcgcagtctg
     3421 cgaacgcttg ccgagatgca caaaccgtct attgcccaga tacacggaaa ctgtctggca
     3481 ggcggcacgg acctcgcgct gtactgcgac attctgatct gcgccgacaa cgcgaacatt
     3541 ggttactcgg ccctgcgcaa catgggcgcg gcgccgaacc agatgtggct gtatcactgt
     3601 ggcccgcaat ggaccaagcg tcttctgctg acaggggaca cgctgagcgg cgaggatgcg
     3661 gccagaatag ggctcgtgct gaagtccgtg cccgaagcgt acctggagag cgaagtggaa
     3721 gggcttgcgg accggttgag ctggatcgat ccggaaatgc tctccaccaa caagcggatc
     3781 atcaatgtgg gcatggagct catgggtgcc caggtgctgc agcggctggc ggcggagaac
     3841 gatgcccggg ctcacaccac gcaggcggcg cgaaacgtgt tcaagcagat agccaccgaa
     3901 gggctgcgct ccgcgcttcg ggaccgggat gcgccgttcg gcgacagccg tgccagggtc
     3961 atcgggccgg aaaccaggga cgagcaggga aggctggtgc cctgggagtg atcagagggc
     4021 ccgcagccgc acccggtggg ctcaggggtt tgcgggaaac tgttcctggt agtcggaaat
     4081 cgacgcatgc agaacccgca gcgcccgctc gcgcccgtac ggctcgccca cgtggacggt
     4141 cgacgattcc ccgggaaggg tcttgtacga ggcgaagtag tgccgcaggc gggcaacgta
     4201 ttgctcgggc aggtcgccaa tgtcgttggt gtcgcgccag atctcgtcgt tcgcatgaac
     4261 cgcgatgatc ttgtcgtcgg cctccccgcc atcgagcatc ggtagcccgc caaccacccg
     4321 tgccgggatg atgacctcgg cgcggttgac cagccggtca ctgatcacgc agatgtccag
     4381 agggtcgaaa tctcccgagt ccgccccttc catcagcact ttcacacggt ctccgcaata
     4441 cgtgcgcggt acgaaaccgt acagcgtcgg tggcagcgag gaggtgcggc tcggccggtc
     4501 cacccgcaga taacccgact ccttgtcgac ctcgtatttc accgtgtcga aaggcgtgat
     4561 ctcgatgaag gcgttgacga caccggagtc gaggtcgccg ggctcgagcc cgtgccacgg
     4621 gtgggggcgc cactctaggt aggtcactgg tcgttctgcg ttggttggtg aaggtggact
     4681 atatctgtgc ccgccggtaa ttggcagatt tggcgtcacc tttcgatggc tgctttttcg
     4741 cggcttaaga cggcttctag ggacggcggg atgcgggacg gggcgaaggt gggcgggctg
     4801 tgctaggctc gcgcgatgtc tacttcgaac acccgtgtcc tgctggcaaa gcggcccgtc
     4861 ggacaggcaa cgcctgactg tttctccgtg gagaccgagc agctgccggc cctcgagcct
     4921 ggccaggtgc gcgtcgccgt tgagtacatc tcggtggatg ccggaacgcg caccatgctg
     4981 cgcggagagg ggtttcatca tcaggtggga ctcgggcaga cgatactcgc gggcggggtt
     5041 ggccacatcg tcgactcggc cgcagatggc tgggaattgg gtcaggcagt tcgcggcggg
     5101 cttggcgcgc agacgatcgc gacggtcacg ccggaattga tcgagcgcgt tgacgaatcc
     5161 gtagcacccc tgagcgcgta tctgggcgtg ctggggggat ccacgggcgt gaccgcctgg
     5221 attggcatgc ggcgcgtcgc caaacctcag ccgggggacg ttttcgtggt gtccgcggcg
     5281 gcaggagcgg tgggatccat tgccggtcag attgccaaac gcgacggcgc gcgcgtcgtc
     5341 ggaatcgccg gcggtccccg aaaggtctcc taccttctcg aacgcctagg gttcgacgct
     5401 gcgatcgact acaagaacga ggacgtcaat gcccgcctgc gcgagctggc gcccgacggg
     5461 gtgaacgtct tcttcgacaa cgtgggcggc cccatcctgg atgccgtgct ggacaacatc
     5521 gcgatgcggg cgagggtcgt catctgtgga gccatctcgc aatacgacga tctggacaac
     5581 gtcaccggcc cctcgctcta ccttcggctg gccgagcgcc agtccaccat ggaagggttt
     5641 gcgttctttc acttccctga gagcattgga ccggcgaccg aggagcttgc gcggtggctg
     5701 gccgacggcg cgctcgtttt cgacgaggag atcctggaag gtatagagcg ctaccccgag
     5761 gcgcttgggt tcatgttcga aggcggcaac ataggcaagc tgctcgtgaa ggcgcgttga
     5821 ccaacggggt cgccgcactt tgcaccctct tttgattcgt aaactcacag gaagccaagg
     5881 tgtctgaaga cttctcactt cgcgaggcga tctacaactg ccgggccatg cgccggatca
     5941 ggcccgaccc cgttccggag gaactcctcg tcgagctgat cgatgcggcg aaccaggcgc
     6001 cgtcgggatc gaactcccag agggcgcgat ggatcgtcgt cagagacccc gcggtcaagc
     6061 gtcagctggc ggaccagaac cgcaggcacg cagagcctta cattgctgcc gacgtctcta
     6121 acccggccac gccgaagctg aagaggatgc tcgatgccgt cgtatggcag atggagcaca
     6181 tgcacgagat accggcgctg atcgtcgcct gtttcgatta ccccgagaag gtggagggca
     6241 tgggcgtgta ccgcagcgcc gggtccgtat ggcctggcat ccagaacctg ctgctgacag
     6301 ctcgcgctct gggccttggc gcggcgccca ccaccctcgc gctccgcgat caggatgccg
     6361 ttcggaaaat tctgaagctg cctgagacct ttgccgcgct ctgcctgatc ccggtcggct
     6421 atccgatggg gaagttcggg cccgtcaccc gaaagcccgt ttctgaggtc atgcggttcg
     6481 acgcctggaa cgactagcgg ccggtatttt gcccgccgga aggaggagaa accgacccgt
     6541 gacgagatgg attgctgttc tgctgatgct gctgtggtgc cggccggcac tcggtggtga
     6601 catcggcgga acctggaaaa acgacgccga tcccgtgtgg atagaggtcg ttcatgacga
     6661 cggcgtggcc accggcaccg tggtccgcaa cgaccgcaat cccgcggcgg ccggccggct
     6721 gctgctcaaa gatctgaagc cgggcgacga agcgaacgtc tggcggggtc aggtcttcgc
     6781 ggtgcggctg ggcgagttca gggacgccca ggtgcgcttg accggcgatg accgcatgga
     6841 aatcctggtc aaggtcggtt tcatgagccg tacagtgacc tggacgcggg ccgaccaggc
     6901 cgtgggcgcg gagtaagtag cctttggggg cgggtcgccg ggcccattac tccttgctgg
     6961 cgaagataga gaaccgcggg cctcgcgggt tcggcacttt ctggccttcc ggtaatcggc
     7021 agattctttc cattctggtc acgctgccgt ccaggtattc
//
LOCUS       DBNHKV010000395        11904 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1188776, whole genome shotgun sequence.
ACCESSION   DBNHKV010000395 DBNHKV010000000
VERSION     DBNHKV010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11904)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11904)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11904
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1188776"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="AC16PP_12665"
     CDS             complement(<1..1110)
                     /locus_tag="AC16PP_12665"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6482526.1"
                     /translation="MVRPEVLEHRDTISKTPILERISAMKESLAAAGDEAQELRHLPA
                     WASKEMADQGLYRFALPLELGGENLCAREQIEVVEAVSAIDGSVGWCTQISSEINALI
                     IRRMEPEFAERIFDDWDALVCSGHGPANGPNPGRLARREGDGWRLNYQGSFASGCHNA
                     NWNYLMGAMTVDEATGKPAQASFMVPLGEFEIIDTWHTAGMRGSGSHDVRMADCYVPP
                     ENLLPFRSLAPSETWQNPTYRNPTHAIYNKAAVALGVCRGAIDSFIELAMEKTPWGLS
                     STLKDQAQVQYRIGEAQATLLAARSFVMETQDKLEEHLGPLPEDGGRLEPEWEYFWPA
                     LLACAHAAQSCRKVVDMINNTAGTTGSRMDSPLERQ"
     gene            complement(1204..1971)
                     /locus_tag="AC16PP_12670"
     CDS             complement(1204..1971)
                     /locus_tag="AC16PP_12670"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6482527.1"
                     /translation="MSSMRGKVALVTGASGGIGLATAIGFAERGAAVMLAARRSDELE
                     RAVREITASGGEAAAIQTDVASSEQVQEMVDRTVGTFGRLDYAVNNAGIEGVPAEFVD
                     FDEDEWDRVMNINLRGNFLCLKYQAKAMLACGHGGAIVNVGSVNSFLGFAGGAAYATS
                     KHGQVALTSSASAELAPKGIRVNIVCPGVIDTPMHRRLRGTFGDEAYDEILSRVHSRR
                     AGRPEEIASTIVFLCSDEASYLTGATITPDGGYLTTM"
     gene            complement(2001..3605)
                     /locus_tag="AC16PP_12675"
     CDS             complement(2001..3605)
                     /locus_tag="AC16PP_12675"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6482528.1"
                     /translation="MSAEFQFGDWTIETSLNRITRADCEQHLEPKTMAVLVFLLNRHG
                     EVVSVDEMLDRLWTGRIVGPNALHRHIALLRRALGDDPSTPSYIETITKRGYRTIASM
                     IRLPPHGAAPSDDSQHDRQEADSWGTRIAVSPFLDRTTGKSHHHLVDGMAEEVVAGLA
                     SFSDIGVIPPSALPRGDASGWDPLDTCAAVGADFFLEGSVSDSASRVRVSLRLTDVAN
                     REVIWSRSFDYVLSASSYFDIQDDIKARVSAGIADSFGVVSSKAYERARTKPTSAIAA
                     DDAVMLAKGHEGNPKLHREAREGLEKAVEQHPDFANAWAWLGAMYRDEFVFWANERPN
                     PLERAESAAQRALKLDASNQEALLVLADVRFYQGDLAAFRQASERAMSLNPSSPDLLS
                     VVASHLIHAGYYEEGKPLAQRAVDLLAQPTLHMLGTLSVCHYGLGDYAAAERVIRQVW
                     AGVDRNRISGMQAYYLTLLVAATAKLGREDEAGQYLQWILKSIPNYAQIMGPSWHRQG
                     ACEDLRERLAEGLAAAGLRIGELTNR"
     gene            3815..4585
                     /locus_tag="AC16PP_12680"
     CDS             3815..4585
                     /locus_tag="AC16PP_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482529.1"
                     /translation="MTMRNKILYTLGFALFVLVWLGVMDARADSHAAVAPSVLESYAC
                     SYKPGKDREDLDKLRDFYLKQAEKAGIAAPPAWLWTLNKGAAPVNFVWLNVHESLTAF
                     GGTDDAIAASPEMAEAFERADTVMTCQAGLGTFRQTYGGEMQESDSSVVSFGCGFREG
                     SGPATMDALHAQIADLNEMAGDLAVNSIFEVTPLTQGPGGSDIVMVVITDGTSGWARH
                     MDYINSIPAGLAAQSHFNAALDCNMNLWRLEQIVGGGD"
     gene            complement(4634..4942)
                     /locus_tag="AC16PP_12685"
     CDS             complement(4634..4942)
                     /locus_tag="AC16PP_12685"
                     /inference="COORDINATES: protein motif:HMM:NF012267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HIR6482530.1"
                     /translation="MTHVVTEACIGVKGEGCMEVCPEFCILSEPADHMSYIDPARCTD
                     CGVCVAACVVGAIFADKDVPRASREFININESWFRHKTGVRRRVRAMASEIGHWLPPE
                     "
     gene            5092..5964
                     /locus_tag="AC16PP_12690"
     CDS             5092..5964
                     /locus_tag="AC16PP_12690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6482531.1"
                     /translation="MKVGLAFASSIGIDGATALDICRRAEAAGFESVWGGEHVIIPDA
                     IASKYPYTPDGKVPVEADTPIPDPLMWLAFAAAAAPTLRLGTCILIVPQRNPLILAKE
                     LATLDQLSGGRVELGLGVGWLKEEFEALGVPWERRGARNDEYIAAMRALWAGPHAEYH
                     GDFVDFAPVTCSPRPVNGSIPVIVGGDTDAAIARAVRLADGYFPGEGDAERLGALLGR
                     LRAAAEQAGRDPASIEVNAMFGVQMADPAAGIEQMAGLGVDRIMIPAFFFAGPDGLDR
                     LSEFGERVMPLTRG"
     gene            complement(5981..6997)
                     /locus_tag="AC16PP_12695"
     CDS             complement(5981..6997)
                     /locus_tag="AC16PP_12695"
                     /inference="COORDINATES: protein motif:HMM:NF016241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HIR6482532.1"
                     /translation="MRHFPLLIAAMLTGILSSHAHATDFVMMKNGDRITGEIKQIWNG
                     EVFIEPEYGDTYPIDLDYVAYVHTDEDFEVEFVEGRRSRTVTGRLGLDDLGRPSVIVG
                     DAAETYPLAKIDNMQEIEDFFEWELATDISVNLATGNSDTRSSRFNVVGKMKLGEHRH
                     RLEYTRDQASAEGDTTKDQTYVAYEDVWTFRDDWFVRGAVNWTRDPIRDLASRSQIFL
                     GPGFHFWDDSKRTLNLSVGPNYLSEDIGGENKQSVAAQLVFRYEQHFLDDDLVVFQET
                     DYQTIVSGRKNKILNTSTGVRWDVLEDVYIKMQVDFDYETNPASAREKEDVTYLVGVG
                     LELD"
     gene            complement(7117..8181)
                     /locus_tag="AC16PP_12700"
     CDS             complement(7117..8181)
                     /locus_tag="AC16PP_12700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6482533.1"
                     /translation="MKAETLLPLGKLDPGLAAPEAALDLHAVAEDARTVDALGYWGLL
                     MEETKDDPFQVLAMAAAATSRVRLGTSVAIAFARSPFVTAMSAWTLQKLSNGRFELGL
                     GSQVRGHIRRRFGLQWHPPGPWMRDYVRAVRAIWASWQNGTKLDFSSQHYEIDLTVPL
                     FTPTPIDHPDIPIFVAAVNAYMCGVAAEVADGVRLHPVCSPRYIRDVVAPAVAEKRPA
                     GKPFEVCLKPLIAAADDGKTLSERAEVARQRLAFYVSTPAYRRPFENAGLGDLAAELS
                     RLSKEQRWHAMAERISDDVLEEWAVVATYDELAATIRSRFDGLVDRVEVGIPVASPED
                     RESLSRIVAELQSGGGSRAS"
     gene            complement(8344..8577)
                     /locus_tag="AC16PP_12705"
     CDS             complement(8344..8577)
                     /locus_tag="AC16PP_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482534.1"
                     /translation="MGIQDLGAIGEFISSLVIVVTLLVLIYEVRGAKNATLQANAQER
                     QPKLDAVDTDLSRQSHQSTSEVYKSGWRGCSDY"
     gene            complement(8731..9189)
                     /locus_tag="AC16PP_12710"
     CDS             complement(8731..9189)
                     /locus_tag="AC16PP_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482535.1"
                     /translation="MDHQAFAEMLGNYGEFLGSVAVLATLLYLAAQIRQNSALAKAQL
                     ETDVMQAFAGAADFPNQHPEVIAKLRAGVDLDDVETVLRDGFVLNYLFACAIGHQASK
                     YADPDRTQRFVMSAALTLGNVDMLLEEHETRLRTSGYEEFVNRVKLELAN"
     gene            complement(9460..10437)
                     /locus_tag="AC16PP_12715"
     CDS             complement(9460..10437)
                     /locus_tag="AC16PP_12715"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HIR6482536.1"
                     /translation="MKICIFGAGAIGGYLGARLAATDVDVSLVARGPHLEAMRRDGLT
                     LREAAEETVVAVNATDDPATLGRQDYVVVTLKAHSVPAIVERLPPLLGPSTAVVTASN
                     GVPWWYFHGLEGPHANRRLRSVDPDDVQWNGIGPERAIGCVVYPACEIEAPGVVRHTE
                     GNRFVLGEPSGEKSERVSALSEAFIDAGLRAPIRRIRDEIWMKLWGNLCFNPISALTT
                     ATLDVIATDSPGTREVAAAMMREAQTIGERLGVRFGVDMDKRIAGAAAVGAHRTSMLQ
                     DLELGRPMEIDALLTAVQEMGEITGVATPTIDVVLALVRQRARIAGCYG"
     gene            complement(10434..>11904)
                     /locus_tag="AC16PP_12720"
     CDS             complement(10434..>11904)
                     /locus_tag="AC16PP_12720"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HIR6482537.1"
                     /translation="AIAAPDSEPCTFADLMDQVERTVGQLNAFGVTTADRAAIVLPNG
                     PEMAAAFVSIACGTTTAPLNPGYREDEFEFYLSDLDPKVLVVETGSVSPARAVAERLD
                     IRTLEIAPHSSGRAGLFDFADAPVPVTPGYGTEDDVALMLHTSGTTSRPKLVPLSHRN
                     LSTSATNIQATLALTPTDRCMNIMPLFHIHGLAAAILASLGAGGSTYCTPGFNALRFF
                     AWLEDAQPTWYTAVPTMHQAILGRTAGNRAAIDAHPLRFIRSCSAALPPQIMQAVEEA
                     FGCPVIEAYAMTEAAHQMTSNPLPPAPRYAGSVGVAAGPEVAIMDDAGNLLGPGETGE
                     VVIKGPNVTSGYIANPKANAEAFHHGWFRTGDQGVMDDGGYLRITGRLKEIINRGGEK
                     IAPREIDEIILEHPAVDQIVTFAMPHDKLGEDVAAAVVLAEGASADAAEIRAFAAERL
                     ADFKVPRKVVFVTEIPKGPTGKLQRIGLAEQLAEALSRS"
BASE COUNT         2032 a         3771 c         3832 g         2269 t
ORIGIN      
        1 ctgccgctcg aggggactgt ccatccggct gcccgtggtg cccgcggtgt tgttgatcat
       61 gtccaccact ttgcggcagg actgcgcggc gtgggcgcac gcgagcaggg cgggccagaa
      121 gtactcccac tccggctcca ggcgcccgcc gtcttcagga agcggcccca ggtgttcctc
      181 cagcttgtcc tgggtttcca tgacgaaaga ccgtgcggcc agcaacgtcg cctgggcctc
      241 gccgatccgg tactggacct gagcctggtc cttgagcgtg gagctcaagc cccagggcgt
      301 tttctccatg gccagctcga tgaacgagtc gatggcgccg cggcagacgc cgagggcgac
      361 ggccgccttg ttgtagatgg cgtgggtcgg gttccggtag gtggggttct gccaggtttc
      421 gctcggtgcg agagaccgga acggcagcag attctcgggc ggcacgtagc agtcggccat
      481 gcgcacgtcg tggctaccgg agccgcgcat gccggcggtg tgccacgtat cgatgatctc
      541 gaactcgccc aggggcacca tgaaggaggc ttgggccggc ttgcctgtgg cctcgtccac
      601 ggtcatcgct cccatcaggt agttccagtt cgcgttgtgg cagccgcttg caaatgagcc
      661 ctggtagttc aaccgccacc catcgccctc ccttcgggcc agccggccgg gattgggccc
      721 gttcgcgggg ccgtgaccgg agcacaccag agcatcccag tcgtcgaaga ttcgttcggc
      781 aaactccggc tccatgcggc gaatgatcag cgcgttgatc tctgacgata tctgcgtgca
      841 ccaaccgacc gacccgtcga tggccgagac ggcctcgacc acctcgatct gctcgcgagc
      901 gcagagattc tctccgccga gttccaaagg aagcgcgaat cgatagagcc cctgatccgc
      961 catctccttg gacgcccagg ccggcagatg acgaagttcc tgggcttcat cgcccgctgc
     1021 cgccagagac tccttcatcg ccgagatgcg ctccagaatg ggcgtcttgg agatcgtgtc
     1081 cctatgctcc agaacttcag gtcttaccat ttcgattttg cctcacaagt cagattgtcc
     1141 cgaagggtga aagaataaac attttgccgc gcacatgccg taattgctcg ggctgccggt
     1201 tgcctacatc gtcgtgagat agccaccgtc gggggtgatg gtggccccgg tcaggtagct
     1261 ggcctcgtcc gagcacagga acacgatggt gctggcgatc tcttcggggc ggccggcccg
     1321 ccggctgtgc acgcggctga ggatctcgtc gtaggcctcg tcgccgaagg tcccgcgcag
     1381 gcggcggtgc atgggggtgt cgatgacgcc ggggcagacg atgttcaccc tgatgccctt
     1441 tggcgcgagt tctgccgatg cagaagaggt gagcgctacc tggccgtgct tggaggttgc
     1501 gtaggcggcg ccgcccgcga agcccaggaa cgagttcacc gagccgacgt tgacgatggc
     1561 cccgccgtgg ccacaggcca gcatcgcttt cgcctggtac ttcagacaga ggaagttgcc
     1621 gcgcagattg atgttcatca cgcgatccca ctcgtcctcg tcgaaatcca cgaactctgc
     1681 cgggaccccc tcgatgccgg cgttgttgac cgcgtagtcc aggcgcccga aggttcccac
     1741 ggtgcgatcc accatctctt gcacctgctc cgacgaggcg acatccgtct ggatcgccgc
     1801 cgcctctccg ccggaggccg tgatctcccg caccgcccgt tccagctcat cggaccgtct
     1861 ggccgccagc atcaccgctg caccgcgctc cgcgaagccg atcgccgtag ccaacccgat
     1921 gccgccgctt gcccccgtca cgagcgccac cttgcctctc atcgaactca tctcccattg
     1981 tccttatgca accgtctagc ctaacggttg gtgagctcac cgatccggag gccggctgcg
     2041 gcgagccctt ccgccaaccg ctcgcgcaga tcctcgcacg ccccttgccg atgccagctg
     2101 gggcccatga tctgagcata gttggggatg ctctttaaaa tccattgcag gtactggccg
     2161 gcttcgtcct cgcgcccgag ctttgcggtg gcggcgacca gcagggtcag gtagtaggcc
     2221 tgcatgccgc tgatccggtt ccggtccacc cctgcccaca cctggcgaat caccctttcg
     2281 gcggcggcgt aatcacccaa gccgtagtgg cacacagaca gcgtgccgag catgtgcagc
     2341 gttggctgtg ccagcagatc cacggcgcgc tgggcgagcg gtttgccttc ctcatagtag
     2401 ccggcgtgaa tcaggtggct cgccaccacg gaaagaagat cggggctgct cggattcagg
     2461 ctcatggcgc gctcggatgc ctgtcggaac gcagccaggt ctccctggta gaaccgcacg
     2521 tccgcgagaa ccagaagggc ttcctggttg gaggcgtcga gtttcagcgc gcgctgtgct
     2581 gcgctctccg cacgctcgag tgggtttggc cgctcgtttg cccagaacac gaactcgtcc
     2641 cggtacatgg cgccgagcca tgcccaggca ttggcgaaat caggatgctg ctcgaccgct
     2701 ttttcaaggc cctctcgtgc ctcacgatgc agtttcgggt tgccctcgtg gcccttggcg
     2761 agcatgacgg cgtcgtccgc tgcaatggcc gaagtcggct tcgtccttgc ccgctcatag
     2821 gccttcgaag agaccacgcc gaaagagtct gcgatgccgg cagagacccg ggcttttatg
     2881 tcgtcctgga tgtcgaaata gctgctcgcc gagagcacgt agtcgaacga tcgggaccag
     2941 atcacttctc ggtttgccac atcggtgagt cgcaggctga cacgaactct cgaggccgaa
     3001 tcgcttacgc tgccttccag gaaaaagtcc gcgccgacgg ccgcgcaggt gtccagggga
     3061 tcccagccgg atgcatcccc cctcggcagg gcgctgggcg ggatgacgcc gatatcgctg
     3121 aagctggcca accccgcgac gacttcctct gccatcccat cgaccagatg gtggtgactc
     3181 tttcctgtcg ttctgtcgag gaacggcgaa acggcgatgc gggtgcccca ggagtcggct
     3241 tcctgcctgt cgtgctgcga gtcatctgat ggcgctgccc cgtgaggggg aagcctgatc
     3301 atggacgcaa tggtgcgata cccgcgcttg gtaatggtct cgatgtacga cggtgtgctg
     3361 ggatcatctc ccagggcgcg gcgcagcagc gcgatatggc ggtggagcgc attgggcccg
     3421 acgatccggc cagtccagag tctgtctagc atctcgtcga cggacaccac ctcgccgtgg
     3481 cggttcagca gaaagacgag cactgccatg gtctttggtt ccaggtgctg ctcgcaatct
     3541 gcgcgtgtga tccggttgag gctggtctca atggtccagt cgccaaattg aaattccgcc
     3601 gacacccgtc tcccccgtct caggtagctt gtcgatccgc tatggcagcg ctgaatgctg
     3661 agcttagcga agggcgcgaa atcagtgaac catcagaaga acatcaggct gttggccgac
     3721 ggctaacgta aattgcctgg agggtgcccc ctgcaggtgt tgtcacggga gactgtagcc
     3781 ggggctggat ggtcgaggtg cacaggaggt tcagttgact atgaggaaca aaattctcta
     3841 cacgttggga tttgctttgt tcgtgttggt ctggctggga gtgatggatg ctcgggctga
     3901 ctcccatgcg gcggtggcgc cgagtgttct ggaaagctat gcctgcagct acaagccagg
     3961 gaaagacagg gaagatctgg acaagctgag ggacttctat ctcaagcagg cggaaaaggc
     4021 cggcattgcc gcgccgcctg catggttgtg gaccctcaac aagggcgctg caccggtaaa
     4081 cttcgtttgg ttgaacgtgc atgaaagcct taccgctttc ggggggaccg atgatgccat
     4141 cgccgcatcg ccggagatgg cggaagcttt cgagcgggca gataccgtca tgacctgtca
     4201 agctggactg ggaacgttca ggcagacgta tggtggagag atgcaggaaa gtgacagcag
     4261 cgttgtttca ttcgggtgcg gattcagaga gggatccggt ccggcgacca tggatgccct
     4321 tcacgcccag atcgctgatc tgaatgagat ggccggcgat ctggcggtca actcgatctt
     4381 tgaagtcacg ccgctgacgc agggaccggg cgggtccgac atcgtgatgg tggtaatcac
     4441 ggacggcacg tccggatggg cccgtcacat ggactacatc aactccatac ctgccgggct
     4501 cgcagcccag agccacttca atgccgctct ggattgcaac atgaacctct ggcgtctcga
     4561 gcagatcgtc ggcggcggag attaatctcg acgcggggcc gaccccatgc gggtcggctc
     4621 ctcgcggagc gcctcattcc ggcggcagcc agtggccaat ttcggaagcc attgctctga
     4681 cgcgcctgcg cacgcccgtc ttgtggcgaa accaggattc gttgatgttg atgaactccc
     4741 gcgatgctct gggaacgtcc ttgtcggcga atatggcgcc gaccacgcag gcggcgacgc
     4801 agaccccgca gtccgtgcag cgggcgggat cgatgtacga catgtgatcg gccggctcgc
     4861 tgaggatgca gaactccggg cacacctcca tgcacccctc gcccttgacg ccgatgcagg
     4921 cttcagtcac gacgtgggtc atcaattctg ttccgttcgt ctgcggtcag ccgatcttac
     4981 cccaagcccg gggctaccct gcgggtttcc gatcgggacc cggaagagat tgggtcctgg
     5041 cgaacgaggc ggtgtaggat gtgcctttca aaccagcagg ggaatcaaac ggtgaaagtc
     5101 ggactagcct tcgcgagcag catcggcatc gatggcgcta ctgctctgga tatctgccgc
     5161 cgagccgagg cggcgggatt cgagtcggtt tggggcggtg agcacgtcat catccccgat
     5221 gccatcgctt ccaaatatcc ctacacgccg gacggcaagg ttccggtgga ggcggatacc
     5281 ccgatccccg accccctcat gtggctggct tttgcagccg cggcggcgcc cacgttgcgt
     5341 ctcggcacct gcattctgat cgtgccccag cgcaaccccc tgattctcgc gaaggagctg
     5401 gcgacgctgg accagctctc cggcggccgg gtcgagcttg gtctcggcgt cgggtggctg
     5461 aaggaggagt tcgaagccct gggcgtgccc tgggagcggc gcggagcgcg caacgacgag
     5521 tacatcgcgg ccatgcgcgc gctttgggcc ggaccacatg cggagtatca cggggatttc
     5581 gtggatttcg cgcccgtgac ctgcagcccg cgtcccgtga acggctcgat tccggtgatc
     5641 gtcggcggcg acaccgatgc ggccatcgct cgcgcggtgc gcctggcgga cggctatttc
     5701 cctggcgagg gggacgcgga gcggctcggt gccttgctgg gacggctgcg tgccgcggca
     5761 gagcaggcgg gtcgggaccc cgccagcatc gaggtcaacg ccatgttcgg cgtgcagatg
     5821 gcagacccgg cggccggcat cgagcagatg gcggggctgg gcgtggacag aatcatgatt
     5881 ccggccttct tctttgccgg accggatggt cttgaccgtc tcagcgagtt cggcgagcgg
     5941 gtgatgcccc tgacccgggg ttgagcgcgc ctcagcggac tcaatcgagc tccagcccga
     6001 cgccgaccag ataggtgacg tcttctttct cgcgggcgct cgccgggttg gtctcgtagt
     6061 cgaagtccac ctgcatcttg atgtagacgt cttcgagcac gtcccatcgc acgcccgtgc
     6121 tggtgttgag aatcttgttc ttccggccgc tcacgatggt ctggtagtcg gtttcctgaa
     6181 aaacgacgag atcgtcatcc aggaagtgct gctcgtagcg gaagaccagc tgagctgcca
     6241 cggattgttt gttctccccg ccgatgtcct ccgagaggta gttggggccg acgctgaggt
     6301 tcagcgtgcg cttggagtcg tcccagaagt ggaagccggg gccgaggaag atctggcttc
     6361 ggctcgccag gtcccgaatc gggtcgcgcg tccagttgac ggcgccgcgg acgaaccagt
     6421 cgtcgcggaa cgtccagacg tcctcatagg caacgtatgt ctggtccttg gtagtgtcgc
     6481 cttcggcgct cgcctggtcc cgggtgtatt cgagccggtg tcgatgctcc cccagcttca
     6541 tttttcccac cacgttgaag cgggaggagc gggtgtccga gtttccggtt gcgaggttga
     6601 cgctgatgtc ggtcgccaat tcccattcga agaagtcctc gatttcctgc atgttgtcta
     6661 tcttggccag cgggtaggtc tccgccgcat cgccgacgat gacggacgga cggcccaaat
     6721 cgtccagccc cagccgtccc gtcaccgtgc ggctgcgccg gccctcgacg aactcgacct
     6781 cgaagtcttc gtccgtgtgc acgtaagcga cgtagtcgag gtcgatggga tacgtatcgc
     6841 cgtactcggg ctcgatgaag acctcgccgt tccagatctg ttttatctcg cccgtgattc
     6901 ggtcgccgtt cttcatcatg acgaagtcgg tcgcgtgggc atggctgctc agaatgccgg
     6961 taagcattgc tgcgatgagc aatgggaaat gtcgcatgtc gggtcctcga gctcgacgaa
     7021 ttcagcggcg cgatgagtgg cgcgctcttc aggggcgggt gttctgccac aattcgctgg
     7081 aaaggtccaa ttctcgtgcg gtggacgagc cccggctcag ctcgcgcggc tcccgccacc
     7141 gctttgcaac tcggcgacga tcctcgacag cgactccctg tcctcggggc ttgcgaccgg
     7201 gatgccgacc tccaccctgt caacgaggcc gtcgaagcgg gaccgaatcg ttgcggcgag
     7261 ctcgtcgtag gtggcgacca ccgcccactc ctccagcacg tcgtcgctta tgcgctcggc
     7321 catcgcatgc catcgctgtt ccttagacag gcggctgagc tcggcagcga ggtcgcccag
     7381 gccggcgttc tcgaacggac ggcggtatgc gggtgtgctc acgtaaaagg ccaggcgctg
     7441 ccgggcgacc tctgcccgct cgctgagcgt cttcccgtcg tcggcggccg cgatcagcgg
     7501 cttcaggcag acttcgaacg gcttgcccgc gggccgtttc tcggccacgg cgggtgccac
     7561 cacgtcccgg atgtagcggg gcgagcagac gggatgcagg cgcaccccgt cggccacctc
     7621 cgcggcgacg ccacacatgt aggcgttgac tgcggcaacg aatatgggaa tgtcgggatg
     7681 atcgatgggg gtcggcgtaa aaaggggtac ggtcaggtcg atttcgtaat gttggctcga
     7741 aaagtcgagc ttcgtgccgt tctgccagct cgcccagatg gctcgcaccg ccctcacgta
     7801 gtcgcgcatc cacggtcccg gaggatgcca ctgcaggccg aagcgtctgc ggatgtggcc
     7861 gcggacctga gaacccaggc cgagctcgaa gcggccgttg gacagtttct gcaatgtcca
     7921 cgccgacatg gcggtgacga atggactgcg ggcgaaggcg atggccacgg aggtgcccag
     7981 gcgcacgcgg ctggttgccg cggccgccat ggcgagcacc tggaacggat cgtccttggt
     8041 ttcctccatg agaagccccc agtagccgag cgcgtccacg gtgcgggcat cttctgcaac
     8101 ggcgtggagg tcgagcgctg cctcgggcgc cgcgagccct ggatccagct tgcccagcgg
     8161 cagaagggtc tcggctttca tgtcggtgtc ctcgtcccgg tcaacgcggt gaaggcgggg
     8221 agagtctaac gaaccggtgg ggggtcgtcg aaacgccctg attggctgat ccttcccgcc
     8281 tggcgccggc ggtaatacgc gcccgcccgt gatctccccc catctggcgg gtttgctctt
     8341 acctcaatag tctgaacaac ctcgccaccc gcttttgtag acttcagaag ttgactggtg
     8401 ggactgccgg gagagatcgg tatctacggc gtctagcttg ggctgccgct cctgtgcgtt
     8461 agcttgaaga gtcgcgttct tcgcgccgcg tacctcgtat atcagcacca agagcgtgac
     8521 gacgatgacg agcgagctga tgaactcccc gatggccccg agatcctgaa tacccattgg
     8581 acgtctccgg ttgtgggtga cggggtttag tccaacgcca gcagtggtcg gatcaactta
     8641 gcttcaatgc gaccggcgct gtttcaattt cgggcaactc atcaagatag gcgcgcaccc
     8701 gctgaccggt tgcggcatct gctgatgtcg tcagttggcc agttcgagct tcactcggtt
     8761 gacgaactcc tcgtaccctg atgtgcgaag gcgagtctca tgctcctcaa gcagcatatc
     8821 gacgttcccc agtgtaagcg ccgcactcat tacgaaccgt tgggtacggt ccggatcagc
     8881 gtacttcgaa gcctgatgac cgatggcgca ggcaaatagg taattcaaca cgaagccgtc
     8941 ccgcaaaacg gtctctacgt cgtccagatc aacgccggcc cgcagcttgg caatgacctc
     9001 cggatgttgg ttcgggaagt ccgcggcccc agcaaacgcc tgcatgacat cggtctcgag
     9061 ttgtgctttc gccagtgcag agttctgtct gatctgcgcc gcgagataga ggagcgtcgc
     9121 taagactgcg accgagccca gaaactcccc gtagttgccc agcatctccg caaaggcctg
     9181 atggtccatg tcggtctcct ctccaatgat tggaacgagg ctacctcagc cgatctgcgg
     9241 ggcaagtctt ctgcgccaac atccttccat gcgcaggcgc cggtgagacg tcgactggaa
     9301 cgtgatcggt gcaatcgggg agcctatcgg ggcagccctt ttccttatct caatgcttta
     9361 tcttgccctg caggtgaaga cggcaaagca gatgatggct gtaaaaaccg ctcggcgttt
     9421 ccgacttccc ggaagaacgg gcggggtccg ggacagcgct cagccgtagc agccggctat
     9481 ccgcgcccgt tgtctgacca gagccagaac gacgtcgatg gtcggcgttg ctacccccgt
     9541 gatctcgccc atctcttgga ccgccgtcag cagcgcgtcg atctccatcg gccggccaag
     9601 ttcaagatcc tgcagcatgg aggtgcggtg tgcgcccacg gcggcggcac ccgcgattcg
     9661 cttgtccatg tccacgccga acctcactcc gagtcgctcg ccgatggtct gcgcctcacg
     9721 catcatggcg gccgcaacct cccgggtccc gggcgagtcg gtcgcgatga cgtccagcgt
     9781 agccgtggtc agggcgctga tggggttgaa gcagagattg ccccaaagct tcatccagat
     9841 ctcgtcgcgg atccgacgga tcggagcgcg aaggccggcg tcaatgaatg cctcgctgag
     9901 cgcggacacc cgctcggatt tctcgccgct gggctcgccg agtacgaagc gattgccttc
     9961 ggtgtggcgg accaccccgg gtgcctcgat ctcgcaggcc ggatagacga cgcagccgat
    10021 ggcccgttcc gggccgatgc cgttccactg cacgtcatcg ggatcgacgc tcctgagacg
    10081 gcggttggca tgcggacctt caaggccgtg aaaataccac cagggtacgc cgttgctggc
    10141 cgttaccaca gccgtggacg ggccgagcag cggcggcaac cgttcgacga tcgccggcac
    10201 cgagtgcgcc ttgagcgtga cgacgacgta gtcctgacgg ccgagggtcg ccggatcgtc
    10261 cgtggcgttg accgccacga cggtctcctc cgcagcctcc cgcagcgtca acccatctcg
    10321 ccgcatggcc tccagatgcg gcccacgcgc gacaagcgac acgtcgacgt cggtcgcggc
    10381 gagccgtgcg ccgagatatc cgccaatggc gcccgctccg aagatacaga tcttcacgac
    10441 cgcgaaagcg cttcggcaag ctgttcggcc agcccgatcc gctgcagctt gccggtcgga
    10501 cctttcggga tctcagtgac gaaaaccacc ttgcgcggca ccttgaagtc ggcgagccgc
    10561 tcggcggcga acgcacgaat ttccgccgcg tcagcactcg caccttccgc aagcaccacc
    10621 gcggccgcga cgtcttcgcc gagcttgtcg tgcggcatgg cgaaggtaac gatctggtct
    10681 actgcgggat gttctaggat gatctcgtcg atctcacggg gcgcgatctt ctcaccgccg
    10741 cgattgatga tctccttcag gcgtcccgtg atccggagat atccgccgtc gtccatcacg
    10801 ccctgatcgc cggtccggaa ccagccgtgg tggaaggcct ccgcgttggc cttcgggttg
    10861 gcaatgtagc cggacgtgac gttcgggccc tttatgacaa cctcgccggt ctcgcccggc
    10921 ccgagcagat tcccggcatc atccatgatc gcgacctccg ggcccgccgc cacgccgacg
    10981 ctgcccgcat agcgcggggc cggcggcaag gggttgctgg tcatctggtg cgccgcctcc
    11041 gtcatggcgt aggcctcgat gaccgggcag ccaaacgcct cctcgaccgc ctgcatgatc
    11101 tggggcggca acgccgcgga gcatgagcgg atgaacctta gcggatgagc atcgatcgct
    11161 gctcgattac cggccgttcg acccaggatc gcctggtgca tggtcggcac cgcggtgtac
    11221 caggtcggct gcgcatcctc gagccaggcg aaaaaccgca gggcgttgaa gccgggtgtg
    11281 cagtaggtcg agccgccggc acccagggag gccagaatgg cggcggcgag cccgtggatg
    11341 tgaaagagcg gcatgatgtt catgcaccgg tccgtcggtg tcagcgcgag cgtcgcctga
    11401 atgttagtcg ccgaggtcga caggttgcga tgggacagcg gcacgagctt ggggcgcgac
    11461 gtcgtgcccg aggtgtgcaa catcagcgca acgtcgtcct cggttccgta gcccggcgtg
    11521 acgggaaccg gcgcgtccgc aaagtcgaaa agcccggcac gaccgctcga gtgcggcgca
    11581 atctcgagcg tgcggatatc gagtcgctcc gcgacggcgc gagccgggga caccgagccg
    11641 gtctcgacca cgagcacctt agggtcgaga tcggagaggt agaactcgaa ctcgtcctcc
    11701 cgatagccgg gattgagcgg cgccgtcgtc gtgccacacg cgatgctgac gaatgccgcc
    11761 gccatctccg gcccattcgg caggactatg gcggcgcgat ccgccgtggt cacgccaaac
    11821 gcattgagct gccccacggt ccgctcgacc tgatccatca ggtcggcaaa ggtgcagggc
    11881 tccgagtccg gcgcggcgat ggca
//
LOCUS       DBNHKV010000396         5332 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1473496, whole genome shotgun sequence.
ACCESSION   DBNHKV010000396 DBNHKV010000000
VERSION     DBNHKV010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1473496"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /locus_tag="AC16PP_12725"
     CDS             <1..1609
                     /locus_tag="AC16PP_12725"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6482538.1"
                     /translation="ATEMPDYARGDWLVYSFSSQVPSLTPYMSGDAYASSIQQWIIDG
                     LVTRDPVTLEWLPHVAESWNISEDGLTISFVVRDGVVFSDGEPLTAEDVAFSWRFVMD
                     EKIAAPRARAYLSRIDDVTVDGRTVTFRFTEPYFGSFSLAGNLGILPRHFYSKYLESV
                     AAAEEYNTSTGLLLGSGPYRLPSPTAWKPGDLIELVRNERYWGWVPAPFDRRIWKTIN
                     SDAAQLTEFKNGGIDVYAALPLEYRDLTQNEEIMKRTRSYEYYNPRGGYSYIAWNNLR
                     DGKPTAFADRRVREAMTYLTDRERVIDEVLLGYAQPANGPFNPLGKQADPSLSVRGYD
                     LDKAKALLAEAGYLDRDGDGVLESEAGEPLAFKLTYPSGSDNYKRIMLLLKDLYVQAG
                     VLMEPDPVDWPVLINTLNTKNFDAVSLAWTSGFEIDIYQMLHSSQTEPGGDNFISYIN
                     PGLDELIETARQEMDEEARMAIWHQVHREIWAEQPYTYLFRTKSLIFADRRIRNVQVV
                     RAGLNEGGLWNMPMEWYVPTDAQKYSD"
     gene            1643..2716
                     /locus_tag="AC16PP_12730"
     CDS             1643..2716
                     /locus_tag="AC16PP_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6482539.1"
                     /translation="MVNYVIRRLLLIFPTLLGITILVFAVMAAAPGGVGASLLSRSGD
                     LRPEERAAMERYINERYGLDKPMPMQYLRWLNNVSPIGFRKDENNEDAGFGFKVPDLG
                     MSFAKNRPVTQLVAQALPITLLLNALSVPLVFILSIVMGVYTARFRGQALDVGIGTAN
                     LALWSIPTIWMGVLLIGLLASEEYARIFPTSGLESLRADRWTFLPVVTEQGWQRGYLL
                     DVVWHLVLPIVCLTYTGFAVLTKLTRSAVLENLQADYARTARAKGASERTVLWYHVFR
                     NSLLPLLTVAASILPGMLVGSVIVESIFSIDGMGRLVVEAVQLRDREVVLSITLITSL
                     LTLLSYLIVDICYAAADPRVSFE"
     gene            2713..3900
                     /locus_tag="AC16PP_12735"
     CDS             2713..3900
                     /locus_tag="AC16PP_12735"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6482540.1"
                     /translation="MTETAITRAVEKVGEESFSRRVTRDTLRNTGAKIGIAWVATVAV
                     LAVFAPFIANSHPYLMRVEGMWSSPLLAHLSWADVTMLVTFLAVVAAWRLRRRFGPGQ
                     LLVGVGVISLVTLLAAQQLVQVPRAVIYDQYRVGLAEGGIETAYFAPVPFSPTDRSRD
                     NPAPLHEPDSVHWFGTDEDGADVLSRMIHACRIALGIGLVATGIALALGIVYGGIMGF
                     FSGWLDIAMMRFLEIFESIPSLFLLLTIVAFFGRNIYLIMVVIGLTSWPGYARFLRAE
                     FLKLRNQDYVQAAEACGLPLASILFRHMLPNGVAPLLVAASFGIASAILAEATLSFLG
                     LGLIDEPSWGAMLNQAVGPTGTFALWLATYPGLAIFFTVFAYNLIGEALRDAIDPHLE
                     QRP"
     gene            3897..>5332
                     /locus_tag="AC16PP_12740"
     CDS             3897..>5332
                     /locus_tag="AC16PP_12740"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6482541.1"
                     /translation="MSALLKVDHLVTRFESGGEATDAVRDVSFEIKPGETVCLVGESG
                     SGKSVTGLSILGLLPDSAVHPNGTITLSTSGRPEVSLLEAKEETLQKVRGARISMIFQ
                     EPMTCLNPVLTIGEQLVEPLELHLGLMGEAARNRAVDLLELVEMPEPERRLDEYPHRL
                     SGGQRQRVMIAMALACEPDLLIADEPTTALDVTIQAQILKLMADLQRKRNTALLFITH
                     DLSVVSEIADRVLVMQAGRIVESGTRDQVLFEPQHPYTKELLAALPANLERLAPPRER
                     TSEPLIRVQNLCVYFPVRKGVLQRHVDDFKAVDHVSLDINKGRVLAVVGESGSGKTTL
                     GRAILGLIKPTAGSVLFAGVDLAGLSRRALKPYRRELQIVFQDPQSSLNPRLSIASIL
                     TEPMAAHGIGGNQRERLERAADLMQKVDLDPMMLGRFPHEFSGGQRQRIGIARALALE
                     PKFIVCDEVTSALDVSVQARILKLLDQL"
BASE COUNT          894 a         1868 c         1653 g          917 t
ORIGIN      
        1 ggccaccgag atgcccgact atgcccgggg cgactggctc gtgtactcgt tttcgagcca
       61 ggttccgagc ctgacaccct atatgtccgg tgacgcctac gcgtcgagca ttcagcagtg
      121 gataatcgac ggcctggtga cccgggaccc ggtcaccctg gagtggctgc cccacgtggc
      181 cgaatcgtgg aacatcagcg aggacgggct gacgatcagc ttcgtcgtcc gcgacggcgt
      241 ggtcttctcc gacggcgagc ccctcaccgc ggaggacgtg gctttcagct ggcgcttcgt
      301 catggacgag aagatcgccg cgccgcgggc tagggcctac ttatcccgca tagacgacgt
      361 caccgtcgac ggccgcacgg tgaccttccg cttcacggag ccctacttcg gttccttctc
      421 tctggccggg aacctgggca ttctgcccag gcacttctac tcaaagtatc tggagagcgt
      481 ggccgcagcc gaggagtaca acacctccac cgggctgctg ctcggctcgg ggccgtaccg
      541 gctgccctcg ccaaccgcct ggaagccggg cgatctcatc gagctggtgc gcaacgagcg
      601 ctactggggc tgggtacccg cgcccttcga ccggcgtatc tggaagacca tcaactccga
      661 cgcggcccag ctcaccgagt tcaagaacgg cggcatcgac gtttacgccg cgctgcccct
      721 ggaatatcga gatctcacac agaacgaaga gatcatgaag cgaaccaggt cgtacgagta
      781 ctacaacccg cgcggcggtt actcctacat cgcctggaac aacctgcgcg acggcaaacc
      841 caccgcgttc gccgatcgca gggtccgaga agccatgacc tacctgacgg accgggagcg
      901 cgtcatcgac gaggtcctgc tcggctacgc ccagcccgcc aacggcccgt tcaaccccct
      961 gggcaagcag gccgacccga gcctgtccgt tcgcggctac gatctggaca aggcgaaggc
     1021 cctgctcgcc gaagccggct acctggaccg cgacggcgat ggggtcctcg agtccgaggc
     1081 cggcgagccg ctggccttca agctcaccta cccctccggc agcgacaact acaagcgcat
     1141 catgctgctg ctcaaagacc tttacgttca ggccggcgtg ctgatggagc ccgacccggt
     1201 ggactggcct gtactgatca acacgctgaa caccaagaac tttgacgcgg tgagcctcgc
     1261 gtggacatcc ggattcgaga tcgacatcta ccagatgctg cattcttcgc agacagagcc
     1321 cggcggggac aacttcatca gctacatcaa cccggggctg gacgagctca tcgaaaccgc
     1381 ccgccaggag atggacgaag aggcccgcat ggccatctgg catcaggtgc accgcgagat
     1441 ctgggccgag cagccttaca cctatctgtt ccggaccaag tcgctgatct tcgcggatcg
     1501 gcgcatccgc aacgtgcagg tggttcgcgc cgggctcaac gagggcggac tgtggaacat
     1561 gcccatggaa tggtacgtgc ccaccgacgc gcagaagtac agcgactgac ggcaggctga
     1621 gaaggaagca gagcgcaaca cgatggtcaa ctacgtcatc cggcgcctgc tgctgatatt
     1681 cccgaccctg ctgggcatca ccatcctcgt cttcgccgtg atggcggctg cccccggtgg
     1741 cgtgggcgcc agcctgctct cccgctccgg agatctgcga cccgaggagc gcgccgccat
     1801 ggagcgctac atcaacgagc gttacggcct ggacaagccg atgcccatgc agtacctgcg
     1861 ctggctcaac aacgtctcgc ccatcggctt ccgcaaggac gagaacaacg aggacgccgg
     1921 gttcggcttc aaggtgcccg acctcggcat gagcttcgcc aagaaccgtc cggtgactca
     1981 gctggtagcc caggcgctgc ccatcacgct cctgctgaac gcgctttccg tgcccctggt
     2041 gttcatcctg tccatcgtga tgggcgtcta cacggcacgt ttccgggggc aagccctcga
     2101 cgtgggcatc ggcaccgcca acctggcgct gtggtccatt cccaccatct ggatgggcgt
     2161 gctgctcatc ggcctgctgg ccagcgagga gtacgcgcgc atctttccca catccggcct
     2221 cgagagtctg cgcgccgacc gctggacgtt cctgccggtg gtcaccgagc agggctggca
     2281 gcgcggctac ctgctggacg ttgtctggca cctggtgctg ccgatcgtct gcctgaccta
     2341 caccggcttc gccgtgctca ccaagctgac ccgctcggcg gtgctggaga acctgcaggc
     2401 agactacgcg cgcaccgccc gggccaaggg cgcctcggag agaacggtgc tctggtatca
     2461 cgtcttccgc aacagcctgc tgccgctgct gaccgtagcc gcctccatcc tgccgggcat
     2521 gctggtgggg tccgtcatcg tggagagcat cttctccata gacggcatgg gccgtctcgt
     2581 ggtcgaggcc gtgcagctgc gggaccggga agtagtgctg tcgatcaccc tcatcaccag
     2641 cctgctcacg ctgctgtcct acctcatcgt cgacatctgc tacgccgcgg cggatccgag
     2701 ggtcagcttc gaatgaccga gaccgccatc accagagctg ttgagaaggt gggagaggaa
     2761 tcgttctccc gccgcgtgac ccgggacacc ctgcgcaaca cgggcgcgaa gatagggatc
     2821 gcctgggtcg ccacggtggc cgttctggcg gtattcgcgc cgttcatcgc caactcgcac
     2881 ccctatctga tgcgcgtgga aggcatgtgg tccagccccc tgctcgccca cctgagctgg
     2941 gcagacgtca ccatgctcgt caccttcttg gccgtcgtcg ccgcctggcg cctgcgccgc
     3001 cgattcgggc ccggccagct gctcgtcggc gtcggggtga tcagcctggt gacgctgctg
     3061 gctgcccagc agctcgtcca ggtgccccgt gcggtgatct acgaccagta ccgggtcggc
     3121 ctggccgaag gcgggatcga aaccgcctac ttcgcacccg tgccgttctc gccaaccgac
     3181 cggtcccggg acaaccccgc ccccctgcac gaaccggaca gcgtgcactg gttcggcacc
     3241 gacgaggacg gcgccgacgt gctcagccgg atgattcacg cctgccgcat cgcccttggc
     3301 atcggcctgg tggccaccgg catcgcgctc gccctcggca tcgtttacgg cggcatcatg
     3361 ggcttcttca gcggctggct ggacatcgcc atgatgcgct ttctcgaaat attcgagtcc
     3421 atcccgtcac tgttcctgct gcttaccatc gtggcgttct tcggccgcaa catctatctg
     3481 atcatggtgg tcatcggcct gacgtcgtgg cccggctacg cccgcttcct gcgtgccgag
     3541 ttcctgaagc tgcgcaacca ggactacgtg caggcggcgg aagcctgcgg gttgccgctg
     3601 gcttcgatcc tgttccgcca catgctgccc aacggcgtgg cgccgctgct ggttgccgcc
     3661 agcttcggca tcgcctcggc catcctggcc gaagccacct tgagctttct cggtctcggg
     3721 ctcatcgacg agccgagctg gggcgccatg ctcaaccagg ccgtcgggcc caccggcacc
     3781 tttgcgctgt ggctcgccac ctatcccggg ctcgccatct tcttcaccgt gttcgcctac
     3841 aacctgatcg gcgaagccct gcgcgacgcc atcgaccccc atctcgagca gcggccgtga
     3901 gcgcgctgct caaggtcgac catctggtca cgcgcttcga gtcggggggc gaggccaccg
     3961 acgcggttcg cgacgtctcg ttcgagatca agccggggga gacggtctgc ctggtggggg
     4021 aatccggctc cggcaagagc gtcaccggcc tctccatcct gggcctgctg ccggattccg
     4081 ccgtgcaccc gaacggcacc atcacgctca gcaccagcgg caggcccgag gtctcgctgc
     4141 tggaggccaa ggaggagacg ttgcagaagg tgcgcggcgc gcgcatctcc atgatctttc
     4201 aggagcccat gacgtgcctg aacccggtgc tcaccatcgg cgagcagctg gtggaacccc
     4261 tggagctgca tctcggcctc atgggcgagg cggcccgcaa ccgggccgtg gatctgctgg
     4321 agctggtgga aatgcccgag cccgagcggc gcctggacga gtacccgcac cggctctccg
     4381 gcggtcagcg ccagcgcgtg atgattgcca tggcgctggc ctgcgagccg gatctgctca
     4441 tcgccgacga gcccaccacg gctctggacg tgaccatcca ggcccagatc ctgaagctca
     4501 tggccgacct gcagcgcaaa cgcaacaccg cgctgctctt catcacccac gatctcagcg
     4561 tggtctcgga gatcgccgac cgcgtgctgg tcatgcaggc gggccgcatc gtggagtcag
     4621 gcactcggga tcaggtgctt ttcgagccgc aacatcccta caccaaggag ctgctggcgg
     4681 ccctgcccgc aaacctcgag cggctcgcac cgccccggga acgcacgtcc gagccgctca
     4741 tccgggtgca gaacctctgc gtgtacttcc cggtccgtaa aggcgtgctg cagcgccacg
     4801 tcgacgactt caaggccgtg gaccacgtca gcctggacat caacaaaggc cgggtgctcg
     4861 ccgtggtggg cgagtcgggc tcgggcaaga ccaccctggg cagagccatc ctcgggctca
     4921 tcaagcccac agcggggtcc gtactcttcg ccggcgtcga tctggccggt ctctcgcgcc
     4981 gggcgctcaa gccctaccgc cgggagctgc agatcgtctt ccaggaccca cagtcgtcgc
     5041 tgaacccgcg cctgtccatc gcctccatcc tcaccgagcc catggccgcc cacggcatag
     5101 ggggcaacca gagggagcgg ctggaacgcg cagcagatct catgcagaag gtggacctgg
     5161 atcccatgat gctcggccgc ttcccccacg agttctccgg cggccagcgc cagcggatcg
     5221 gcattgcgcg ggcgctggcg ctggaaccga agttcatcgt ctgcgacgag gtcaccagcg
     5281 cgctggacgt cagcgtccag gcgcgcatcc tgaagctgct ggaccagctc aa
//
LOCUS       DBNHKV010000397         3957 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_836011, whole genome shotgun sequence.
ACCESSION   DBNHKV010000397 DBNHKV010000000
VERSION     DBNHKV010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_836011"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="AC16PP_12745"
     CDS             <1..590
                     /locus_tag="AC16PP_12745"
                     /inference="COORDINATES: protein motif:HMM:NF015684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR6482542.1"
                     /translation="ESVSDDQRELQGVFMAERRPDRQDVTVWAESGRQVVDPDTGSRF
                     LVLNNGTRYEGRIGDRNYRVVEFGALGQRLALQPAAQRSTEVESLPTRTLMGAGRPDE
                     IAELHWRYSLPLLVLIATGIAVGLGRVKPRQGRFAKIVPGILVFVGYYVALVAVRNAL
                     EDGVWPPALGLWPVHLAFLGVSVALLTAASRPAKI"
     gene            594..1670
                     /gene="lptG"
                     /locus_tag="AC16PP_12750"
     CDS             594..1670
                     /gene="lptG"
                     /locus_tag="AC16PP_12750"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HIR6482543.1"
                     /translation="MLAVMDRHIGRTVLATTMMVLIAFLGLMTIFALIDELGGSRESY
                     GFGEALTYVALTTPRRIYEMLPYMVFLGALIGLGSLASNSEIVVLRAAGVSVWRVFLS
                     VAWPAFIVLLLGFLLGEFVAPRGEELAEAHKTRSRQDSEVIVLRGGYWYREANLYMNV
                     DGLDGQGGLIGLRQFWLDAEENLVRSVSASRAEYLPGADPHWMLFDVVETAFQDESTS
                     VQRSESARWDGNVDPRLLGVRVLVEPRKLSVTDLSYQIDYMTREGLNSGSYQLAYWGK
                     LLQPLSVLGLALLALCFVLGPLREVGMGVRLSVGVIAGLSFKYLQDLFAPMSYVYDLP
                     PPLAVALPIIVCWAIGLVGLRRVA"
     gene            complement(1675..2145)
                     /locus_tag="AC16PP_12755"
     CDS             complement(1675..2145)
                     /locus_tag="AC16PP_12755"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR6482544.1"
                     /translation="MPTGGHISEATPTDANGQPGATRRHAGLLRRVGAMCYDALLVVA
                     LWLVTLFPIVALSNSAVTGATVRSLLFIELFVFFAYFWVSRGQTLGMLAWRLRLVPAA
                     PGEPVTLRQALLRFIGALAAFATLGLGYLWMYIDPERRTWPDMLSGTRVIHEPR"
     gene            complement(2120..3577)
                     /locus_tag="AC16PP_12760"
     CDS             complement(2120..3577)
                     /locus_tag="AC16PP_12760"
                     /inference="COORDINATES: protein motif:HMM:NF036848.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HIR6482545.1"
                     /translation="MSNTPILVGVAQVEQRIEDVREGEEPLAMMIDATRLAARDAGCP
                     GLLSEANSVRVIRGMWPYANPARAVGEAIGNPGAETALTPFGGNSVQSTLNHSAIDIR
                     DGVHDVIIITGAECGRSQAKAGRAGVDLNWSEVPGTPDRYIGEDMEMRCPAERGLGMG
                     RPIQMYPMMENHLRYRRGDTIEEQLIRISELWASFSAVAAENPHAWMREAKTAEQLRT
                     ISVENRPVSFPYPKLMNSNNNVDQGAALILCSTEKAKALGIPEEKWVYPWAGTDAHDT
                     YFVSNRDSMSRSPAIRLAGRRCLELTGLTPLDLAFVDVYSCFPVAVQVAAEEIGLDTT
                     RPLTITGGLTFAGGPLNNYVMHSIATMVEKLRGARGEKGLITANGGYLTKHAFGVYGT
                     EPPATPFQHEDLQPAVDATATRKVVTEWTGEATVESYTVMYKGDELAVGHLSCLTPDG
                     VRTWANCEDPDTLLAMTREEFCGRPVRLADRRAHF"
     gene            3699..3785
                     /locus_tag="AC16PP_12765"
     tRNA            3699..3785
                     /locus_tag="AC16PP_12765"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3733..3735,aa:Leu,seq:caa)
BASE COUNT          631 a         1228 c         1334 g          764 t
ORIGIN      
        1 cggaatccgt cagcgacgac cagcgtgagc tgcagggcgt gttcatggca gagcgccgcc
       61 cagaccgcca ggacgtcacc gtatgggccg agtccggtcg gcaggtcgtg gatccggata
      121 ccggcagccg gtttctcgtg ctcaacaacg gcacccgtta cgaggggcgc atcggagaca
      181 ggaactatcg ggtcgtggag ttcggcgcgc tcgggcagcg gctcgccctg cagccggcgg
      241 cccaacgcag cacggaggtg gaatctcttc cgacccggac gctgatgggg gcaggccggc
      301 cggatgagat tgcagaactg cattggcgct actccctgcc gctgctggtg ctcatcgcca
      361 cgggaatcgc cgtggggctg gggcgcgtga aaccgcggca ggggcgcttt gcgaagatcg
      421 tcccgggcat tctggtcttc gtggggtatt acgtcgcgtt ggtcgcggtc cgcaatgccc
      481 tggaggatgg agtctggcca ccagcgctgg ggctctggcc cgttcacctg gcgttcctgg
      541 gcgtctccgt tgctctgctc accgccgcgt cgcggccagc gaagatctga tccatgctgg
      601 cggtaatgga cagacacatc ggccggaccg ttctcgcgac gaccatgatg gtgctgattg
      661 ccttcctggg actgatgacg atcttcgcgc tcatcgatga gcttggcggc agccgcgaat
      721 cctacggttt tggcgaagcg cttacgtacg tggcgctgac cacgcctcgg cgcatctacg
      781 agatgctccc gtacatggtg tttctcggtg ccctcatcgg gcttggcagc ctggcaagca
      841 attcggagat cgtcgtgctg cgcgcggccg gcgtctctgt atggcgggta ttcctgagcg
      901 tggcgtggcc ggcgttcatc gtgctgctgc tcgggtttct gctcggtgag ttcgttgccc
      961 cccgggggga ggaactcgcc gaggcgcaca agacgcgcag cagacaggac tccgaagtga
     1021 tcgttctgcg cggtggctac tggtatcggg aagcgaacct ttacatgaac gtggacggcc
     1081 tggacgggca gggcgggctc atcggtctgc gccagttctg gctggacgca gaggagaatc
     1141 tggtgcgctc ggtgtccgcg tcccgggcag agtacctgcc cggcgccgat ccgcactgga
     1201 tgctcttcga tgtggtggaa accgcgtttc aggatgaatc cacgtccgtg caacgcagcg
     1261 aatcggctcg ctgggacggc aacgtcgatc cgcgtctgct gggggtcagg gtgctcgtcg
     1321 agccccgcaa gctgtcggtc accgacctga gctatcagat cgactacatg accagggagg
     1381 ggctcaactc gggttcctat cagctggcct actggggcaa gctgctgcag ccgctctccg
     1441 tgctcggcct tgctctgctc gcgctctgct tcgtcctggg ccctttgcgc gaggtaggca
     1501 tgggcgtgcg gctgagcgtc ggtgtcatcg cgggtttgtc cttcaagtat ctccaggacc
     1561 tcttcgcgcc catgagctac gtctacgacc tgccgcctcc gctagcggtg gctctgccca
     1621 ttatcgtctg ctgggccatc ggcctggtgg ggctgcgccg cgtggcctga gcgatcagcg
     1681 gggttcgtgg attacccgtg tgccggacag catgtctggc cacgttcggc gctcggggtc
     1741 gatgtacatc cacaggtatc cgagacccag ggtggcgaac gcggcgaggg cgccgatgaa
     1801 gcgcagcagt gcctgtcgca gggtgacggg ttccccgggt gcggccggta ccaagcgcag
     1861 tcgccaggcg agcatgccga gcgtctggcc ccggctcacc cagaagtagg caaaaaaaac
     1921 gaagagttcg atgaacagga gggatcgcac cgtggcgccg gtgacggcgg agttgctcaa
     1981 cgcaacgatt gggaacagcg tgacgagcca gagcgcgacg acgagcagcg cgtcgtagca
     2041 catggcgccg acgcgccgca gcagcccggc atggcggcgc gtggcgcccg gttgcccgtt
     2101 ggcgtccgta ggcgtagcct cagaaatgtg cccgccggtc ggcaagtcga acgggcctgc
     2161 cgcagaactc ctcccgggtc atggccagga gcgtgtccgg gtcctcacag ttagcccagg
     2221 tcctcacacc gtccggggtc aggcagctca ggtgcccgac ggcgagctcg tcacccttgt
     2281 acatgaccgt gtagctctcc accgtggcct cgcccgtcca ttccgtgacg actttccggg
     2341 tggccgtggc atcgacggcc ggctgcaggt cctcgtgctg gaacggcgtt gcaggtggct
     2401 cggtaccgta cacgccgaaa gcatgcttgg tgagatagcc accgttggcg gtgatcaggc
     2461 ctttctcacc ccgggcgccg cgcagtttct ccaccatcgt ggcgatggag tgcatgacgt
     2521 agttgttcag gggccctccg gcgaaggtca gacccccggt gatggtgagc ggccgtgtcg
     2581 tgtccaggcc gatctcttcg gcggcgacct gcacggccac cgggaagcag ctgtagacgt
     2641 caacgaaggc cagatccaat ggcgtcaggc cggtcagctc gaggcagcgt cggccggcga
     2701 gccgaatggc gggcgagcgg ctcatgctgt cccggttcga cacgaagtag gtatcgtggg
     2761 cgtcggtgcc cgcccagggg tacacccact tctcctcggg gatgccgagc gcttttgctt
     2821 tctcggtcga gcagagaatg agggccgcgc cctgatccac gttgttgttg gagttcatga
     2881 gcttggggta gggaaaggag acgggccggt tctccacgga gatcgttcga agctgctccg
     2941 cggttttagc ctcgcgcatc cacgcgtggg gattctccgc ggcgacggcg ctgaacgacg
     3001 cccacagctc ggagatacgg atcaactgtt cctcgatggt atcgccccgg cgatagcgca
     3061 gatggttttc catcatcggg tacatctgta tggggcgacc catgccgaga ccgcgctcgg
     3121 cggggcagcg catttccatg tcctcaccaa tgtaccggtc gggcgtgccg ggaacttcgc
     3181 tccagttcag gtcgacgccc gctcgccccg ccttagcctg actgcggccg cactcggctc
     3241 cggtgatgat gatgacgtcg tggacgccat cgcgaatgtc gatcgcgcta tggttcagcg
     3301 tcgactgcac gctgttgccg ccgaacgggg tgagcgcggt ctccgcgccc ggattgccga
     3361 tggcttcacc cacggccctt gccggattgg cgtaaggcca catgcctctg atcacccgca
     3421 cggagttcgc ctcgctgagc aggccggggc agccagcgtc ccgcgccgcc agtcgcgtgg
     3481 cgtcgatcat catcgccagc ggctcctcgc cttcgcgcac gtcctcgatc cgctgctcga
     3541 cctgggccac gccaaccaga atgggggtat tgctcatgtt ctcgaagaaa tcctgactga
     3601 atcttttaag gggcggggat gataccgaaa ggaagctgcg ctgtcgcaaa ggtgccattg
     3661 ccgcgctttc tcggcctgtg caacaatccg cgatcgctgc ctgggtggcg aaactggtag
     3721 acgcgccaga ctcaaaatct ggtttgggca accaagtgtg ggttcgattc ccaccccagg
     3781 caccagtaaa aatgcgggtt tcaggccggt cccgaaatgt tgcattagaa cgtcagtgct
     3841 ggtgcaacac cggtgcaaca tttcgggcgg tttttgaggt tttcgatgag ctgaaagcta
     3901 tcgttcactc agtgcggtcg cgtctccctg ccctctgaag cggccccctc ctcgacg
//
LOCUS       DBNHKV010000398         3824 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_978399, whole genome shotgun sequence.
ACCESSION   DBNHKV010000398 DBNHKV010000000
VERSION     DBNHKV010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_978399"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..981)
                     /locus_tag="AC16PP_12770"
     CDS             complement(160..981)
                     /locus_tag="AC16PP_12770"
                     /inference="COORDINATES: protein motif:HMM:NF006123.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6482546.1"
                     /translation="MSGDEQKSIFITGGAGGMGMATGRLFAQHGWFVGLFDLDESALE
                     LARAAFDDSQSLVRRLDVTSETDFADAMAAFSERTGGRMDVMFNNAGIAPGAWFDEMP
                     LDTIRQVIDINVMGVIIGIRAALPLLRATPGSLCMSTSSSVATYGHAMRAAYTASKFA
                     VKGLTEALSLEFERFGIRTADVLPGCIDTPMLRNALSARTGEPFDESLFETLPKEGAY
                     RLVPVSAIADAVWNAYHSDVIHWYVPEEVGEIDRLKGADFDAARAETRAFLFGEG"
     gene            complement(1018..1788)
                     /locus_tag="AC16PP_12775"
     CDS             complement(1018..1788)
                     /locus_tag="AC16PP_12775"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6482547.1"
                     /translation="MQTILFESSGGVGTLTLNRPDRLNGMTNQMLIETHDCLLEVAQR
                     DDVRVLVLTGAGRGFCPGADLKHYAAGEADVASQPRHFQVPVLLHEMPQITIAAINGP
                     CAGAGLGWACGCDLRIAARSANFNTAFLKVASAGDMGGPWSLPRLIGAAKARELYFLA
                     EKFGAEEALAMGLVSRVFEDEQFAEKAAAMAGALASSSPHALRTMKQNFLDAERLNFA
                     DYVELETGRHLEVMAHPDAREAFRAFVEKRPPRFESSE"
     gene            complement(1804..2619)
                     /locus_tag="AC16PP_12780"
     CDS             complement(1804..2619)
                     /locus_tag="AC16PP_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6482548.1"
                     /translation="MSGYTDIDVQIEDPACLIRLNRPDKLNAFTYHTLREIRQAIDAA
                     AADSRVVGIIITGNGRGFSAGLDAQVLAEVTSRGGSAADDSAGEDDLPGIFSYLLEVP
                     KPVIAAVNGVAAGGGLILALMSDLRIASRDASFITVFLKRGLVAEHGSSWLLPRLVGT
                     GRALDLLWMSDRIDAQTAADIGLVERLAEPDELIPSALDYVTRLAEGSAPKAIAATKQ
                     LVYRHLGMGYADALKEADRVQRRFVAAADAAEGARALLEKRSPRFERQGTTDD"
     gene            complement(2616..3386)
                     /locus_tag="AC16PP_12785"
     CDS             complement(2616..3386)
                     /locus_tag="AC16PP_12785"
                     /inference="COORDINATES: protein motif:HMM:NF024859.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6482549.1"
                     /translation="MWLRLRQIALVAAKLEPIEAQLESVLGIRVCYRDPGVAHFGLEN
                     ALLPIGNQLLEVVAPIEEGTAGGRYLERRGGDGGYMVITQCDDHGPRRRRVEALGIRI
                     VNQYDSGEFRNMQMHPKDTGGSFFEIDEQLGDGAHDPDGPWEPAGPDWQQGRQLDRVS
                     GIAAAEIQADDPARVAARWSEIAEIPLTTTADGHPALVLDNATVRFVPCTDGRPEGLG
                     GIDVLATNAAAIMATARDQGLATGERQIYLCGMRINLV"
     gene            complement(3410..>3824)
                     /locus_tag="AC16PP_12790"
     CDS             complement(3410..>3824)
                     /locus_tag="AC16PP_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482550.1"
                     /translation="FFQFALILYNKEGPFEPGTVNLWDRYQEWKQSGKGRDLTPNSGL
                     VGSPETIRTRLQQFQDVHIDQVILLNQAGKNRHEDICASLELFAREVMPEFHAAEAGH
                     QEWKAAVLDGSLELEDIDTDQYNIYSLQTPNSKAS"
BASE COUNT          585 a         1268 c         1295 g          676 t
ORIGIN      
        1 ccggctggac aacatccgcc tggcggatcc gggcgaccga ttcctgcacg tccccagcag
       61 cttcctgcgg ggcctcgagc acctcaacct gaaattcgac gtccgcgacc gggagcgtta
      121 tctgccaggg gcgtccgctt actagcgacc cccgcgcgct cagccctcgc cgaacaggaa
      181 agcccgggtc tccgcccggg cggcgtcgaa gtcggcgccc ttgaggcgat caatctcgcc
      241 cacctcctcg ggcacgtacc agtggatcac gtcggagtgg taggcgttcc agacggcgtc
      301 cgcgatggcg gaaaccggca cgagccggta ggcgccctcc ttggggaggg tctcgaacag
      361 ggactcgtcg aagggctcgc ccgttcgcgc cgacagggca ttgcgcagca tgggcgtgtc
      421 gatgcagcct ggcagcacgt cggcggtgcg gatgccgaag cgttcgaact ccaggctgag
      481 cgcttcggtc aggcccttca ccgcgaactt ggaggccgtg taggcggcgc gcatggcgtg
      541 tccgtaagtt gccaccgagg aggacgtgct catgcacagc gagcccggcg tggctcgcag
      601 caggggcagg gcggcgcgga tgccgatgat cacccccatg acgttgatgt cgatgacctg
      661 acgaatggta tccagcggca tctcgtcgaa ccacgccccc ggcgctatcc ctgcgttgtt
      721 gaacatcacg tccatgcgcc cccccgtgcg ctcggagaac gcggccatcg catcggcgaa
      781 gtcggtctcg ctggtgacgt cgagccgccg gacgaggctc tgcgagtcgt cgaaagccgc
      841 ccgcgcaagc tccagggcgc tttcgtcgag gtcgaagagg ccgacgaacc agccatgctg
      901 ggcgaagagg cgtccggttg ccatgcccat gccgccggcc ccgccggtaa tgaaaatcga
      961 tttctgctcg tccccgctca cgttgcgtct cctcgtaatg gtttgtgctt ggtcggatta
     1021 ctcgctggac tcgaaacgcg gcgggcgctt ctcgacgaaa gcccgaaacg cctcccgggc
     1081 gtccgggtgc gccatgacct ccagatggcg gccggtttcg agctccacgt agtcggcgaa
     1141 attcaggcgc tcggcgtcga gaaagttctg cttcatggtt cgcagcgcgt ggggggacga
     1201 gctcgccagc gccccggcca tggcagctgc tttctcggcg aattgctcgt cctcgaagac
     1261 ccggctcacc aggcccatcg ccagagcctc ctcggccccg aacttctctg ccaggaaata
     1321 cagctcccgc gctttcgcgg cgccgatgag acggggcagg gaccagggcc cgcccatgtc
     1381 gccggcggac gccaccttga ggaacgccgt gttgaagttg gcggagcggg cggcgatgcg
     1441 caggtcgcag ccgcaggccc aaccgagacc ggcgccggcg caggggccat tgatcgcggc
     1501 gatggtgatc tggggcatct cgtgcagcag cacgggaacc tggaagtggc gtggctggga
     1561 agccacgtcc gcttcgccgg cagcgtagtg cttcaggtcc gccccggggc agaaaccccg
     1621 gcccgctccg gtgagcacca gcacgcgcac gtcgtcccgt tgcgccactt cgaggaggca
     1681 gtcgtgggtc tcgatcagca tctggttggt catgccgttc agccgatccg ggcggttcag
     1741 ggtcagcgtg cccacgccgc ccgatgattc gaagaggatg gtctgcatgg gcttcggctc
     1801 cgttcagtcg tctgtcgtgc cctggcgttc gaagcgggga gagcgcttct cgagcagggc
     1861 gcgcgcgccc tcggcggcgt ctgcggccgc cacgaagcgg cgctgcaccc ggtcggcttc
     1921 cttcagcgcg tcggcatagc ccatgcccag gtgccggtag acgagttgct tggttgcggc
     1981 aatggccttg ggcgccgagc cctccgccag gcgcgtgacg taatcgaggg cgcttggaat
     2041 cagctcgtcg ggttccgcga gcctctccac caggccgatg tcggccgccg tctgcgcatc
     2101 gatccggtcg ctcatccaga ggaggtccag cgcgcggccg gtgccgacga ggcgcggcaa
     2161 cagccagctc gacccgtgct cggccacgag cccgcgtttc agaaagacgg tgatgaagct
     2221 tgcgtccctg gaagcgatcc gcaggtcgct catcagcgcc agaatgagcc cgccgccggc
     2281 cgccacgccg ttcacggcgg cgatgacggg tttgggcact tccagcaggt agctgaaaat
     2341 tcccggcagg tcgtcttcac ccgcggaatc gtcggccgcg ctcccgcctc tcgaggtgac
     2401 ctctgccagc acctgggcgt cgaggccggc ggagaacccg cggccgttgc cggtaatgat
     2461 gatgccgacc acccgggaat cggctgccgc ggcatcaatg gcctggcgga tctcccggag
     2521 cgtgtggtag gtgaaagcgt tcagcttgtc cgggcggttc agccggatca ggcaggcggg
     2581 gtcctcgatc tggacatcga tgtccgtgta gccgctcata ctaggttgat gcgcatgccg
     2641 cacaggtaga tctgccgctc gccggtggcc aggccctgat cgcgtgcggt ggccatgatg
     2701 gctgcggcgt tagtggcgag cacgtcgatg ccgccaaggc cctccgggcg accgtcggtg
     2761 cagggcacga agcgtaccgt cgcgttgtcc agcaccagcg ccgggtggcc gtccgccgtg
     2821 gtggtgagcg ggatctccgc tatctcggac cagcgcgcgg ccaccctggc ggggtcgtct
     2881 gcctggatct cggctgcggc gattccgctc acgcggtcga gctgccggcc ctgctgccaa
     2941 tcggggcccg ctggctccca ggggccgtcc gggtcgtgag ccccgtcacc gagctgctcg
     3001 tcgatctcga agaaggatcc tccggtgtcc ttggggtgca tctgcatgtt gcggaactcg
     3061 ccgctgtcgt actgattgac gatgcggatg ccgagcgcct ctacccggcg ccgacgaggc
     3121 ccgtgatcgt cgcactgggt gatgaccatg tacccgccgt caccgccgcg ccgctccagg
     3181 tagcggccgc ctgccgtgcc ttcttcaatg ggcgccacga cctccagcag ctgattgcca
     3241 atgggtagca gcgcgttttc caggccgaag tgggcgacgc cgggatcgcg atagcagacc
     3301 cgtatgccaa gaaccgactc cagctgcgcc tcgatgggct ctagctttgc agccaccagg
     3361 gcgatctggc gcagtcgtaa ccacatgttt cctccgttaa cggaaatgct caggacgctt
     3421 tgctgttcgg cgtctgaagc gagtagatgt tgtactggtc cgtgtcgatg tcctccagct
     3481 ccagggagcc gtctaggacg gcggccttcc actcctggtg cccggcttcg gcggcgtgga
     3541 actcgggcat cacctccctg gcgaagagct ccaggctggc gcagatgtcc tcgtggcggt
     3601 tcttgccggc ctgattgagc aggatgacct ggtcgatgtg cacgtcctga aactgctgca
     3661 gacgtgtccg gatggtttcc ggtgagccca cgagccccga gttcggtgtc aggtcacggc
     3721 ctttgccgga ttgcttccac tcctggtatc ggtcccacag attcacggtg cccggctcga
     3781 agggtccctc cttgttgtag aggatgagcg cgaactggaa gaac
//
LOCUS       DBNHKV010000399         3011 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1331453, whole genome shotgun sequence.
ACCESSION   DBNHKV010000399 DBNHKV010000000
VERSION     DBNHKV010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1331453"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /gene="dgcA"
                     /locus_tag="AC16PP_12795"
     CDS             <1..1029
                     /gene="dgcA"
                     /locus_tag="AC16PP_12795"
                     /inference="COORDINATES: protein motif:HMM:NF042940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-D-Glu racemase DgcA"
                     /protein_id="HIR6482551.1"
                     /translation="KTFRTESRRLPMDVFAHIESWPIAGTFTIARGSKTVAEVVVVTV
                     REGDLTGMGECLPYPRYGQSPEGTLEEIRVATAGHAILDRQRLTELMPAGPARNALDC
                     ALWDLEARKSGQPVWRLAGLAEPRPVVTALTIVLGEPGQMREAAERHSRRSLLKIKLG
                     AGAGRDIERLRAVRSGAPDARLIVDANEGWTLGELEQVAPVAADLGVEMIEQPLPVSE
                     DEALDAFRCPVPLAADESLRAGEDLDRLAGRYQVANIKLDKTGGLTEALILQKKAETL
                     GMDVMVGCMVATSLSMAPALLLAQTARFVDLDGPLLLVRDRQPGLDYHDSLVAFPQQA
                     VWGAGEHG"
     gene            1055..1990
                     /locus_tag="AC16PP_12800"
     CDS             1055..1990
                     /locus_tag="AC16PP_12800"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR6482552.1"
                     /translation="MNSSASDRVIWRNGEFVPWADATVHVLAQSLQRGTLAFDFMSVH
                     ETADGPAIFRLEAHIERLLQSCRLEELPILYPAEALMEACAETVRRNPGCKSVKISAL
                     IASIEVELIPQDPAVAVFIAAYDSARDIMAKHQTPYPTRETLALKVEREISNRRHDIL
                     PPQAKVAANYTSPTIAKRKARAEGFDDVLLLTERGTVAEAPTSNIFVVRDGGLATPPE
                     NDVLLGITRASIIEIAGFLNLPCEEQELELTQLYAANEAFLTNTSTGVWPIERVDDVV
                     FGDGAPGPVTKRLRAALRKISSGEEPTFRHWLHYV"
     gene            complement(2026..2529)
                     /locus_tag="AC16PP_12805"
     CDS             complement(2026..2529)
                     /locus_tag="AC16PP_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482553.1"
                     /translation="MATRRRTLAMGLALLAGGYLGPLARHARAQSLEQAMEESDLIYL
                     TPLKSDGSESRCRAEVWFARHEADLFVVTSSDAWRARAVERGLTRTRVWVGDVGVWSG
                     SGGSYRTLPQIEADASIVAEADVHDAVLDVMGDKYRMSWLVWGPRFRNGLADGSRVML
                     RYRPAAA"
     gene            complement(2564..>3011)
                     /locus_tag="AC16PP_12810"
     CDS             complement(2564..>3011)
                     /locus_tag="AC16PP_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011335679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HIR6482554.1"
                     /translation="GEPLPVDFTNRIIYYVGPVDPVRDEIVGPAGPTTATRMDKFTGM
                     MLEKTGLIGMIGKAERGPAGVDAIREHGAVYMIAVGGAAYLVAKAITRSTPLAFEDMG
                     MEAIYEFEVKDMPVTVAVDSTGASVHREGPRLWSQKIADRIGAAQL"
BASE COUNT          540 a         1006 c          966 g          499 t
ORIGIN      
        1 aaaactttcc ggactgaaag ccgccggctc cccatggacg ttttcgcaca catcgagagt
       61 tggcccatcg ccggcacttt taccatcgcc cgaggcagca agacggtggc cgaggtggtc
      121 gtggtgaccg tgcgtgaggg cgacctgacc ggcatgggcg aatgtctgcc ctatccccgc
      181 tacggacagt ctccggaggg aaccctggaa gagatccggg tcgccacagc cggccacgcc
      241 atcctggatc gtcaacggtt gacggagctg atgcctgccg gcccagcgag gaacgcgctg
      301 gactgcgcgc tgtgggatct ggaggcgcgg aaatccggac agccggtctg gaggctcgca
      361 gggcttgcag agccgcggcc cgtggtcacg gcgctgacca tcgtgctcgg cgagcccggg
      421 cagatgcgtg aagcggctga acggcactct cgccgttcgc tgttgaaaat caagctcggc
      481 gcgggtgccg ggcgggacat cgagcgcctg cgcgcggtgc gcagcggcgc cccggacgcc
      541 cgcctgatcg tcgatgccaa cgaaggctgg acgctgggag agctcgagca ggtcgctccg
      601 gtggcggcag atctgggcgt ggagatgatc gagcagcctt tgccagtatc cgaggatgag
      661 gctctggacg cttttcgctg ccccgtcccg ctggcagccg acgaaagtct gcgcgccggt
      721 gaggatctcg accggctcgc tggccgctac caggtcgcga acatcaagct cgacaagacc
      781 ggcgggctca ccgaagctct gatcctgcag aagaaggcgg aaacgctcgg catggacgtg
      841 atggtgggct gcatggtggc gacgtcgctc tccatggcac cggccctgct tttggcgcag
      901 actgcgcgct tcgtggacct ggacggaccg ctgctgctcg tgcgcgaccg gcagccgggt
      961 ctcgactacc acgacagcct ggtggctttc ccccagcagg ccgtttgggg tgccggcgaa
     1021 cacgggtaga gaagcttaag gaaacgacga ccacatgaat tcctcagcat ccgatcgggt
     1081 gatctggcgc aacggcgagt tcgtgccctg ggcagacgct accgtgcacg tgctcgccca
     1141 gagcctgcag cgcggcaccc tggccttcga cttcatgagc gttcatgaaa ccgcggacgg
     1201 gcccgcgatc ttccgcctgg aagcccacat cgagcgtctg ctgcaaagtt gccgcctgga
     1261 ggaattgccc atcctgtatc cggccgaggc gctgatggaa gcctgcgcgg agacggtgcg
     1321 tcgaaacccc ggctgcaaat ccgtgaagat cagcgcgttg atcgcctcca tcgaggtcga
     1381 gctcatacct caggatcccg ctgtggccgt attcatagcg gcgtacgaca gcgcgagaga
     1441 catcatggcc aagcaccaga ccccgtatcc gacacgggag accctggccc tgaaagtaga
     1501 gcgcgagatc agcaatcggc gtcacgacat cctgccgccc caagccaagg tcgcagcaaa
     1561 ctacacctca ccgaccatag ccaagcgcaa agccagggcc gaaggttttg acgacgtcct
     1621 gctcctgacg gagcgcggga ccgtggcgga agcgccaacg tccaacattt tcgtggtgcg
     1681 ggatggcggg ctggcgacgc ccccggaaaa cgatgtgctg ctcggcatca cccgcgcatc
     1741 gatcatcgag atcgctggat tcctgaacct gccctgcgag gagcaggagc tggaactgac
     1801 gcagctctac gcggccaacg aggcgttcct caccaatact tccacgggcg tgtggcccat
     1861 cgagcgcgtt gacgacgtgg tgttcggcga cggggcgccc ggcccggtga ccaagagact
     1921 acgtgccgcc ctcagaaaga tctcgtccgg cgaggagccc accttccgcc actggctgca
     1981 ctacgtctga cctcacggcc agaagcggtc gatgttcgtg gtcggtcagg cggcagcagg
     2041 acggtaccgg agcatgaccc gcgacccgtc cgcgagaccg ttcctgaatc ggggacccca
     2101 gaccagccag gacatccggt acttgtcacc catgacgtcg agcaccgcgt cgtgcacgtc
     2161 ggcctccgcc acgatcgagg catccgcttc gatctgcggc aacgttcggt agctgccgcc
     2221 gctgccggac cagacgccca cgtcgccaac ccaaacccgg gtgcgcgtga gccctcgctc
     2281 gacggctctc gcccgccagg cgtcgctcga cgtgacgacg aagagatcgg cttcatgacg
     2341 cgcgaaccag acctccgccc ggcagcggct ctccgagcca tcgctcttga gaggagtcag
     2401 gtagatcagg tccgattcct ccatcgcctg ttccagcgac tgcgcccggg cgtgacgggc
     2461 gagagggccg aggtatcccc cggcgagcag cgccaggccc atcgccagcg ttctccttct
     2521 tgttgccatc acacagcctc cgccggatag tgaagtcggg tcgtcacaac tgggcagcgc
     2581 ctatgcgatc ggcgattttc tgcgaccaca gcctgggtcc ttcgcgatgc accgatgcgc
     2641 cggtcgaatc caccgccacc gtgaccggca tgtctttgac ctcgaactcg tagatggcct
     2701 ccatccccat gtcctcgaag gccagcgggg tgctccttgt gatcgctttg gcgacgaggt
     2761 aagcggcgcc acccacggcg atcatgtaaa ccgcgccgtg ctcgcggatg gcatcgaccc
     2821 cggccggacc cctctcggcc ttgccgatca tgccgatcag ccccgtcttc tccagcatca
     2881 tgccggtgaa cttgtccatg cgcgttgccg ttgtcggccc cgcggggccc acgatctcgt
     2941 cgcgtactgg gtccacgggg cccacgtagt agatgatgcg attggtgaaa tccacgggta
     3001 gaggctcgcc a
//
LOCUS       DBNHKV010000400        18239 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_340812, whole genome shotgun sequence.
ACCESSION   DBNHKV010000400 DBNHKV010000000
VERSION     DBNHKV010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 18239)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18239)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18239
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_340812"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="AC16PP_12815"
     CDS             complement(<1..444)
                     /locus_tag="AC16PP_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HIR6482555.1"
                     /translation="MPDFDKEGLRNVLLVAVSVCLVCSVIVSSAAVILKPRQLINQEL
                     DEKQNILRAAGMLPQGSNQDMQGRGIDELFDQFTVHAVDLETGQFAELDEIDAYDPIK
                     AAKQASRSRELSADEDIATIGRRENVSLVYLRRDSDGVLDKVVIPV"
     gene            complement(437..1621)
                     /locus_tag="AC16PP_12820"
     CDS             complement(437..1621)
                     /locus_tag="AC16PP_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HIR6482556.1"
                     /translation="MSLRSFLDDVAPHFEKGGRFAWAFPAYEAVDTALYTPGRVTGGP
                     SHVRDGLDLKRIMITVWLCAFIPVFAGSYVVGLQANEVMQSLGIESVDNWRGLFLDNL
                     TSYDPTSIWDCVLHGLAYFLPLYVTVFVAGIVFEIWFASVRGHEVNEGFFVTSILFTL
                     TLPATTPLWMAAVGIVFGVVIGKEVFGGTGKNFLNPALTGRAFLYFAYPAQMSGDAVW
                     VAVDGYTRATALGNAALGESYAVDWLAAFLGNMAGSLGETSTLAILLAGVILVASRIA
                     SWRIMAGVTAGMVAMSVVLNLIESNNPMFAMPFYWHFVLGGFAFGMVFMATDPVSAAM
                     TNAGRWIYGGLIGVMCVLIRVVNPAFPEGMMLAILFGNLFAPLIDHFVVQRNVNRRLA
                     RA"
     gene            complement(1622..2965)
                     /locus_tag="AC16PP_12825"
     CDS             complement(1622..2965)
                     /locus_tag="AC16PP_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HIR6482557.1"
                     /translation="MIKIKRGLNLPISGSPEQTIETARPARSVALVGGDYPGMRPTML
                     VREGDEVRCGQPLFEDKKNPGVCFTAPASGRVASINRGAKRALQSVVIDVGSAPALEF
                     TAYGPDGMAGLTRSQVVDNLVASGLWTALRTRPFSKIPSPDSEPHSLFVTAIDTNPLA
                     ADPGVVIPEYATEFAAGLDVLAHLTSGAVHVCQESGAFLPAGNDPKLSVQEFAGIHPA
                     GLPGTHIHFLDPVGPSKTVWFIGYQDVAAVGRLFLTGEIMIDRVVALGGPGVEKPRLL
                     KTVLGVNLEELTAGELAEGDQRIISGSVLSGRACAGPVGYLGRYHLQVSVVPEDKERR
                     LFGYLTPGLKKHSVFPIYLSKWLGGADVEFSTTTNGSPRGMVPIGTFESVMPLDVLPT
                     QLLRALLVGDLETAISLGCLELDEDDIALCTYVCPGKYEYGPVLREVLTQIEKEG"
     gene            complement(3165..4634)
                     /locus_tag="AC16PP_12830"
     CDS             complement(3165..4634)
                     /locus_tag="AC16PP_12830"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR6482558.1"
                     /translation="MALPSLDDYFPDWKEREALAEAMIPIIGRLYRNNVVTYCYGRAL
                     YNQSVIQIMKTHRYVRQVAQNELSEFESYPIIEALSQLDLGPSHIDVGKLATGFMEEA
                     AGNGLSPMEYVRRECQEVIGRHVPPLSKPQDVVLYGFGRIGRLLARLLIEKTGSGHQL
                     RLRAVVVRKGAPDDLVKRASLLRRDSVHGGFNGTIRVDEENSCIIANGNVVHVIYANS
                     PDQLDYTEFGIHDAIVIDNTGVWRDEEGLGLHLKSKGASKVVLTAPGKGSIKNVVSGI
                     NSSIIEPTDTILAAASCTTNAIVPPLKAMNDQFGIVHGHMETVHAYTNDQNLIDNFHK
                     KNRRGRSAALNMVITETGASTAVTKLLPELDGKLTGNAIRVPTPNVSLAILNLTLSRD
                     TSVAEVNEHLRNVSLHSSMQRQVDFTEAPDVVSSDFVGSRHACVIDGGATLVKDNRAV
                     LYVWYDNEFGYACQVVRVVQKWAGISYPLIPKDVIKQGF"
     gene            complement(4738..5793)
                     /locus_tag="AC16PP_12835"
     CDS             complement(4738..5793)
                     /locus_tag="AC16PP_12835"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR6482559.1"
                     /translation="MTFVVKPRVHHPKLPVNEIGLTRRDYEGPVSTLCAGCGHDSVSA
                     AVVQACAELSLPPHKFAKVSGIGCSSKTPSYFLSKSHGFNSVHGRMPSVATGANLANR
                     ELICIGISGDGDSASIGLGQFAHIVRRRINMVYIVDNNGTYGLTKGQFSATNDKGSTN
                     KKGVPNLYEPIDLVSMALQLGASFVARSFSGDKRQLVPLIKAALTHNGFAFLDVISPC
                     VAFNNHEGSTKSYDYVRGHSENINVLDFIPEGVEITADYEPGTTEDVRMPGGSILRLR
                     KLDGAYDPQDRVEALHYVQQQQAEGEVVTGLLYVDPDASDCHEIMDTVQRPLNELNED
                     DLCPGDDALEAVNESFR"
     gene            complement(5793..7622)
                     /locus_tag="AC16PP_12840"
     CDS             complement(5793..7622)
                     /locus_tag="AC16PP_12840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR6482560.1"
                     /translation="MTKRTIVNDFVIRFANVNGSGSASANGMFAKALFRMGIPVATRN
                     IFPSNIQGLPTWFEVRVSERGYLGRREGVDIMVAMNAETFEEDVASLNPGGYLVYDNT
                     KALPASLRRSDVHEVGIPISSMLLSEFSDPKQRSLFKNITYLGALTALLNIEFDVITG
                     MVSTQYKGKDALIGPNIHALELGRDYARDYLECPLPFQVKRADLNGDKIMVDGNTAAA
                     LGALYGGATVCAWYPITPSTSVAEEFERFANRMRIDGQSGQRKFAIIQAEDELSAIGI
                     VIGAAWNGARSFTATSGPGISLMTEFLGLAYFAEIPAVVFDIQRSGPSTGMPTRTQQG
                     DLISSAYASHGDTKHVLLFPSTPRECFDHAALALDLADRLQTPVIVMSDLELGMNDSI
                     SEPFEWDDERRYDRGKVLDREQLDSLERFGRYLDVDGDGIGYRTLPGTHPDKGAFFTR
                     GTSRDEFAAYTESPDVYVANMERLSAKWRTAARLVPSAEIESNGERAGIIYYGTSVAP
                     MREALDMLEEEGIRLDTMRITAFPFGAEVYDFIAAHDTVFVVDQNRDAQMKVLLVTEG
                     ELDPAGLVSVLYYGGLSISADTIQKQVSDYFTEQRLPRLKEVK"
     gene            complement(7619..9403)
                     /locus_tag="AC16PP_12845"
     CDS             complement(7619..9403)
                     /locus_tag="AC16PP_12845"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6482561.1"
                     /translation="MKPTNILDPNYFHKVVDCQWACPAHTPVPEYIRLIAQQRYTEAY
                     MLNWDSNVFPGILGRTCDRPCEPACRRVRTEEKPVAICRLKRVAADHKGDISAYLPKV
                     PERKNGKRIALLGAGPASLAVARDLLPFGYEVHLFEKEPMGGGMMRSQIPAFRLPVGV
                     LEEEVDVILDMGVECHFGHEVSSMKEMLDEDFDAYFIGTGAPRGRDLELPGRAEVAEH
                     ISIGIDWLSSVAFGHTHSISGKVIVMGGGNTAMDCCRTAKRLGAESVSVVVRSAFEVM
                     KASEWEKEDAMSEGIPIINNRPPKEFVHENGKLKGVVFECVKPVKGDDGRLRYEPSGE
                     PDVFMEADHILMAIGQENHCPWIERDIGIQFDKWDCPMLDEETLQSTHPKVFFGGDSA
                     FGPENIIWASAHAHKAAISIHLMCQGKDLKADRPPEGVNLISQKMGVHEWSYDSEHDP
                     ARRYLVPHADKRLALADIKMEVELGFDEELGAKEAQRCLNCDVQTVFVDQLCIECDAC
                     VDICPMDCITFTENAEEPDLRRRLTAPAGNVEQDLYVSDELNTGRVMVKDEDVCVHCG
                     LCAERCPTNAWDMQKSIIHIPQLGSYAE"
     gene            9755..13216
                     /gene="mfd"
                     /locus_tag="AC16PP_12850"
     CDS             9755..13216
                     /gene="mfd"
                     /locus_tag="AC16PP_12850"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR6482562.1"
                     /translation="MSPFQPPTPPAVGSRIAWNELHDGAAGLAVGRAAAAHEGLTLMV
                     AESTRQAQRWLDELRFFAPDVHLLHFPDGETLAYDAFSPHQDIISERLSTLRDLPSQR
                     TEREPTLLVVPVQTLLQRVAPRTYLDGHSLSLRTGQHFDLDAQRGRLNAAGYNAAETV
                     SAHGEYAVRGSLMDIYPMGADLPIRIDLFDDEVESLRTFDPDTQRTVEKIDAIRLLPA
                     KEFPLDGKAIARFRSNWHNTFNVDVRRCSVYQDISEGISPNGIEYYLAFFFEELATLF
                     DYLPANTVVIREAGVREAAERFLHDVGVRYESLRHDVERPILPPSDVYLRREELNAAL
                     KAWPQIVINDASARHAVDFATQACGDLEANPRLRDPARGLCEFLDGITQPVLFVADSA
                     GRREVFLEFLNAAGIRPRETADLPAFRDSRLDCAIAVAPVEQGLWTDELVIVTESQVF
                     GHQLEERRARSARVLDPEQIIRNLTELHVGDPVVHIEHGIGRYLGLTTLEIDGAPSEF
                     LAVAYAEDAKLYVPVTSLHLISRYAGAEHAPLHRLGSDAWDKAKRKAAERAHDVAAEL
                     LNIYARRASHSSYRFNDPSADYRRFAAQFPFELTPDQGRAIDEVIADLCSERATDRLI
                     CGDVGFGKTEVAMRAAFLAVQSNKQVAVLVPTTLLAQQHFETFRDRFADWPVQIEALS
                     RLRSESEVEAIAERMASGRIDVIIGTHKLLNPALSFNDLGLVIIDEEHRFGVRQKERL
                     KALRAEVDVLTLTATPIPRTLNMAMSGMRDLSIIATPPAKRLSIKTFVQEKRQHVIKE
                     AVTRELMRGGQVFYVHNEVRTIERAAAELAELVPEARIGVGHGQMPKRQLERVMNDFY
                     HRQLNVLVCTTIIETGIDIPNANTIIMERADKLGLAQMHQLRGRVGRSNRQAYAYLLT
                     PHPKAITKDAVKRLEAIEAAGELGVGFTLATHDMEIRGAGELLGEEQSGQIESVGYSL
                     YMDLLDRAVKAIQDGKTPNLNAPLEPIAQEVNLHTPTLIPEHYLPDVHARLILYKRIA
                     NAADPTELDDLQAEMIDRFGLLPEPLKSLFAVTALKLKTQPLGVVRVDLGESGGRIEF
                     SPDTEVDPLAIVNLVQSHPGTYRLDGATMLRINRTMPDFQARITFAGELLDRLAAHTP
                     NRLVANA"
     gene            13222..14157
                     /locus_tag="AC16PP_12855"
     CDS             13222..14157
                     /locus_tag="AC16PP_12855"
                     /inference="COORDINATES: protein motif:HMM:NF022425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsiV family protein"
                     /protein_id="HIR6482563.1"
                     /translation="MSPRRAAATAGLAAALLGQASDAMEAEEPVDLLLKDWYVTEIIV
                     VQRPPVMDFNSAERLLRDRPPALPLAMRTFATDPSQLGKDLDLDLSTRLSLVHPTLAL
                     LPPAQDEADVDEGSGAMVAPAIEPVLEPDPLLDVLAMAAALEEQLEARSFQLIPAEEL
                     TMSASADRLARRSGFQVLYHGAWLQPVPPRDAPEYLLLQTGARVADRHQLEGVVAVTL
                     GRYLHFQASFSYTEPGMGLEPMTLVLNPDGSGTPARWPVETRPVMIMNQSRRMRSEEV
                     HYLDHPKLGIIVRIDPVPVPEELVAAFDALEEPTE"
     gene            complement(14116..14856)
                     /locus_tag="AC16PP_12860"
     CDS             complement(14116..14856)
                     /locus_tag="AC16PP_12860"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HIR6482564.1"
                     /translation="MRRLAVLVGSGALKLPFDVLVEDFRDTPYGEASSKPSRAAGRNF
                     IVHVLARHGVPHAVAPHLINYRANLHLLSELGVEEIVALNTVGGIAKHARNGRIVLPD
                     QIIDYTWGRAHSFAAEGDLKHVDFTEPYDAALRAELLAAARGQGLDVHDGGVYGCTQG
                     PRFESPAEIDRMDRDGCTVVGMTGMPEAGLARELGIRYASLCLVVNPAAGRGDAIDLD
                     HIAVIAREGMEDVARLLGGFFQGIERGD"
     gene            complement(14856..15428)
                     /locus_tag="AC16PP_12865"
     CDS             complement(14856..15428)
                     /locus_tag="AC16PP_12865"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF006605.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HIR6482565.1"
                     /translation="MTARVPTEILTAREAAELVVGAQEVQAAIDRTAVRLAVALEAAN
                     PLVLCLLNGGLPFTAELIKRFSFPLELSYLHATRYGQRTSGSQLEWLARPPVSVAGRH
                     VLLVDDIFDAGETLMQVVEWLRREGAVEVTTAVLVDKAVPRSGAARVDYAALCCPDRY
                     LFGCGMDFRGYWRNLPAIYALPRSLEDEAV"
     gene            complement(15425..16492)
                     /locus_tag="AC16PP_12870"
     CDS             complement(15425..16492)
                     /locus_tag="AC16PP_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6482566.1"
                     /translation="MLESLRDFLSQNTWVVEVFAIVFATALVRYAVRRAFERLAGRLE
                     QTHNIYDDALFDAARGPVGVGIWVLGVSWAAEVAGAVGGAEIFDYVDEVREVAIIWLL
                     VWFALRFIRFMEVHLADSSYREEPVDETTAAAVGKLLRAAAIITGVLMAMQEFGFSIA
                     GVLAFGGVGGIAVGFAARDLLANFFGALMIFLDRPFSVGDWVRSPDREIEGTVEDIGW
                     RLTRIRTFDQRPLYVPNSIFTNLAVENPSRMRNRRIYETIGVRYEDVGVLRQIVADVE
                     SMLREHPDIDHGRTLMVNFVEFGPSSLDFFVYTFTKTTVWAEFHAIKQDVLFRIADII
                     EEHGAEIAFPTQTLHVVQPES"
     gene            complement(16509..17717)
                     /locus_tag="AC16PP_12875"
     CDS             complement(16509..17717)
                     /locus_tag="AC16PP_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482567.1"
                     /translation="MAAQPASTDGIEPSSADHSLDLDLGLQLRAFSKSGLDGQSRTQG
                     SVSAQLEYYRAWNEDRSSLTFTPFVRVDGEDDERTHFDVRELFWSRVGPDWDLHLGAK
                     QVFWGVTEFKHLVDVINQTDLVENIDGEDKLGQPMAHLSVVRDWGILDFYLLTGFRER
                     TFPGSDGRLRPPLPVDDDARYESGAGRWRVDGAVRWQRQLGRLTLGIHHFSGTSRAPV
                     LIPETGPAGQAVLRPFYPVIDQTGLDAQAIVGDWAWKLEAVSSSGYEGRYSAFNAGFE
                     RSLVGVLGGRGDLGLVVEYMFDDRDERAFDTLFEHDLALGTRWQMNDVADTQALIGVI
                     WDVKTDEYVVKLEGSRRLGDTWTLLVEGRIFGGSERPMPGVPPSTDPNNRAWPLERDD
                     FLQLELTRYF"
     gene            complement(17780..>18239)
                     /locus_tag="AC16PP_12880"
     CDS             complement(17780..>18239)
                     /locus_tag="AC16PP_12880"
                     /inference="COORDINATES: protein motif:HMM:NF028441.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HIR6482568.1"
                     /translation="PAMKRVKKIASQNKSGPFLSSEFAYEDLTVQEIEKFSYRFLGQG
                     QANGIDCFVVERTPRDDFSGYSRQQVWIDQDELRVLRIEYFDRRDSLLKTLTIDQYAR
                     YEDQFWKAGRMYMTNHQTDRSTELFWRNYRFRTGLMDERDFSTNSLLRVR"
BASE COUNT         3431 a         5945 c         5650 g         3213 t
ORIGIN      
        1 aaccgggatc accaccttgt ccaacacgcc gtccgagtct cgacgcaggt agacgagaga
       61 gacgttctcg cgccggccga tggttgcgat gtcctcgtct gcggacagct cacgcgagcg
      121 gctcgcctgc tttgcggcct tgatggggtc gtatgcgtcg atctcgtcga gctcagcgaa
      181 ctgaccggtc tcgagatcca cggcatgcac ggtgaactga tcgaaaagct cgtcgatgcc
      241 gcggccctgc atatcctgat tgctgccctg cggcagcatg ccggcggccc gcaggatgtt
      301 ctgtttctcg tcgagttcct ggttgatcag ctgccggggt ttgaggatca cggccgccga
      361 ggacacgatc accgagcaca ccaggcagac cgataccgcc accagcagca cgtttcgcag
      421 tccttccttg tcgaaatcag gcacgagcca gtctccggtt gacgttgcgc tgcactacga
      481 aatggtcgat gagcggggca aataggttgc caaagaggat ggcgagcatc atgccttccg
      541 ggaaagccgg gttgaccacg cggatgagca cgcacatcac cccgatgagc ccaccgtaaa
      601 tccaccgccc tgcattggtc atcgcggcgg agaccgggtc cgtggccatg aacaccatgc
      661 cgaaggcgaa gcccccgagc acgaagtgcc agtagaacgg catggcgaac atggggttgt
      721 tcgattcgat caggttcaag acgacggaca tggccaccat tcccgccgtc acgccggcca
      781 tgatgcgcca ggaggcaatt cgggacgcca cgagaatgac cccggcaagc agaatcgcaa
      841 gggtcgatgt ctcgcccagg gagcccgcca tattgccgag gaaggcggcg agccagtcca
      901 ctgcgtaaga ttctcccagg gccgcgttgc ccagcgcggt cgcccgcgtg tacccgtcta
      961 cggcaaccca caccgcatca ccggacatct gcgccgggta ggcgaagtaa aggaacgccc
     1021 gccccgtgag agcgggattc aggaagttct tgccggtgcc accgaatacc tccttgccga
     1081 tgaccactcc gaaaactatc ccgacggcgg ccatccatag cggcgtcgtg gcgggcaacg
     1141 tcagggtgaa cagtatgctc gtgacgaaga agccttcgtt gacctcgtgc ccgcgaacgg
     1201 aggcgaacca gatctcgaac acgattcccg ccacgaagac ggtcacgtac agaggcagga
     1261 agtacgccag gccatgcagg acgcagtccc agatgctggt cggatcgtag ctcgtcagat
     1321 tgtcgaggaa gaggccgcgc cagttgtcca cggactcgat ccccagggac tgcatcacct
     1381 cgttggcctg cagccccacg acgtagctac cggcaaaaac cggaataaac gcgcacagcc
     1441 acaccgtgat catgatccgc ttgagatcca gcccgtcacg gacatgggag gggccgcccg
     1501 tcactctccc aggggtatac agagccgtgt cgacggcctc gtaggccgga aatgcccagg
     1561 cgaaccggcc gcctttctcg aagtgtgggg caacatcgtc gaggaaagat cgcaagctca
     1621 tctagccctc cttctcgatc tgcgtgagca cttcgcgaag aacgggaccg tactcgtatt
     1681 tgccgggaca cacgtacgtg cacagcgcga tgtcgtcctc gtcgagttcc agacagccca
     1741 gactgatggc cgtttccaga tcgcctacga gcagagcgcg aagcagttgc gtcggcagca
     1801 cgtccagcgg cattacggat tcgaaggtac cgatgggaac catgccccgc gggctgccgt
     1861 tcgtggtggt ggagaattcc acgtctgcgc cgccgagcca cttcgacagg tagatgggaa
     1921 aaacggagtg ctttttcaaa cccggcgtga ggtaaccgaa aagccggcgc tccttgtctt
     1981 ccggtactac cgacacctga aggtggtaac gaccgaggta ccccaccgga ccggcgcagg
     2041 cccgcccgga aagcacggaa ccggagatga tgcgctggtc gccctcggcg agctcgccgg
     2101 ccgtgagttc ctcgagattt acgccgagca cggttttgag cagccgcggc ttctcgacgc
     2161 ccggcccacc cagggccacc acgcgatcga tcatgatctc accggtcagg aacagcctgc
     2221 ccaccgcagc gacgtcctgg tagccgatga accagacggt tttcgacggt ccgaccggat
     2281 ccagaaaatg aatgtgagtg ccgggaaggc cagcgggatg gatgcccgcg aattcctgga
     2341 cgctgagctt agggtcgttg ccggctggca gaaacgcgcc cgactcctga cagacatgca
     2401 cggcgccaga ggtgaggtgc gccaggacat cgaggcctgc agcgaactcc gtggcgtact
     2461 cggggatcac caccccggga tccgccgcca gcgggttggt gtcgatggcg gtcacgaaaa
     2521 gggagtgggg ctcggaatcc gggctgggga tcttggagaa cggcctggtc cggagggccg
     2581 tccacaaccc gctcgcgacg agattgtcca ctacctgaga gcgggtgagc ccagccattc
     2641 cgtcggggcc gtaagcagtg aactcgaggg ccggggcgga ccctacatcg atcacgacgg
     2701 actgcagcgc acgtttcgcg ccgcgattga tgctggccac ccgacccgag gccggcgccg
     2761 tgaaacacac gcccggattc ttcttgtcct cgaaaagcgg ctgcccgcag cggacctcgt
     2821 cgccttcgcg caccagcatg gtggggcgca tgcccgggta gtcgccaccg accaaggcca
     2881 cgctccgggc gggccgagcg gtctctatgg tctgctcggg gcttcctgaa atgggaaggt
     2941 tcaatcccct tttaatcttg atcatgttcc gaaccaacaa tggccagggt tcttgcgctt
     3001 tcgcggactg cgctcggccc actcaccgga gggcagcggg aaaatacggc tgttggaggc
     3061 accgaaagga acgcctcggc aggccgcgcg aattatagtg gggtcgtgtc cgcatgccaa
     3121 cgcctgggcg gacctgatac gcattcttag gggaaatttt gccttcagaa tccctgtttg
     3181 atcacgtcct tgggtatgag ggggtaggaa atacccgccc acttctggac cacacgcacc
     3241 acctggcagg cgtatccgaa ctcgttgtcg taccagacgt acagcacggc ccgattgtcc
     3301 ttgaccaagg tggcgccgcc gtcgatcacg caggcgtgcc gactgccaac gaagtccgac
     3361 gagacgacgt cgggcgcttc ggtaaagtcc acctggcgct gcattgaaga atgcagcgag
     3421 acgttgcgca ggtgctcgtt gacctctgcc accgaggtgt cgcgggacag cgtcaggttc
     3481 aggatggcca gagaaacgtt cggagtcgga acccgaatgg cgttaccggt cagcttgccg
     3541 tcgagctcgg gcagaagctt ggtcaccgcc gtggacgctc ccgtctcggt gatcaccatg
     3601 ttcagagcgg cgctgcgacc acgccggttc ttcttgtgaa agttatcgat caggttctgg
     3661 tcgttcgtgt aagcgtgcac ggtctccatg tgcccgtgaa cgatcccgaa ttgatcgttc
     3721 atggctttca gaggcgggac gatcgcgttg gtcgtgcagg aggcggcagc cagtatggtg
     3781 tccgtgggct cgatgatgga cgagttgatc ccggagacga cgtttttgat gctgcccttg
     3841 cccggcgccg tgaggacgac cttcgacgcg cccttggatt tcaggtgcag gcccaggcct
     3901 tcctcgtcgc gccaaacgcc ggtgttgtcg atcacgatgg cgtcgtgaat tccgaactcc
     3961 gtgtaatcca gctggtcggg cgagttggcg tatatcacgt ggacgacgtt gccgttggcg
     4021 atgatgcagc tgttttcctc gtccacccgg atcgtgccgt tgaacccgcc gtgcacggag
     4081 tccctgcgca acaggctggc tcgcttgacc agatcgtcgg gagcaccctt gcgaaccacc
     4141 accgcccgca gcctcagctg atgaccgctt cccgtcttct cgatcaggag ccgggcgagc
     4201 agccggccga tccggccgaa gccgtaaagc accacgtcct ggggcttgga gagcggcggc
     4261 acgtgacggc cgatgacttc ctggcactcc ctgcgcacgt attccatggg cgacaggccg
     4321 ttgcccgcag cctcttccat gaaccctgtc gccagcttgc cgacatcgat gtgggatggt
     4381 cccagatcga gctgcgacag cgcttcgatg atcggatagc tttcgaactc ggagagctcg
     4441 ttctgggcga cctgacgcac atagcgatgc gtcttcatga tctgaatgac gctctggttg
     4501 tagagcgccc ggccgtaaca gtaggtgacc acgttgttgc gatacagacg cccgatgatg
     4561 gggatcatcg cctcagcaag cgcttcgcgc tccttccagt ccgggaagta gtcgtcgaga
     4621 ctcggcaatg ccacaggttc tccctccctg ttaacgattt tcagccgggg ttagggcccg
     4681 gcttggagcc gcgcgtatta tcaaaatttg ccccctgggg cgcaaggaaa ccagggctta
     4741 ccggaaggat tcgttcacgg cctcgagcgc atcgtcgccc gggcagagat cgtcttcgtt
     4801 gagctcgttc agggggcgct gcaccgtatc catgatctcg tgacagtcgg acgcgtcggg
     4861 gtccacgtaa agaaggcccg tgaccacttc cccttcggcc tgctgctgct ggacgtagtg
     4921 cagggcttcg acacgatcct ggggatcgta agcgccatcc agcttgcgca gccggaggat
     4981 cgagcctccg ggcatgcgga cgtcctcggt cgtgccgggc tcgtagtcgg ccgtgatttc
     5041 cacaccctcg ggtatgaaat ccaggacgtt gatgttctcg ctgtgcccgc gcacgtaatc
     5101 gtagctcttc gtggatccct cgtgattgtt gaacgccacg cacggcgaga tcacgtcaag
     5161 aaaggcgaac ccgttgtgag tgagcgccgc cttgatcagc ggcaccagct ggcgcttgtc
     5221 gcccgaaaag cttcgggcaa cgaagctcgc gcccagttga agggccatgc tgacgagatc
     5281 gatgggctcg tagagattcg ggacgccctt cttgttcgtg gaccccttgt cgttcgtcgc
     5341 cgagaactgt cccttggtca gaccataagt gccgttgtta tcgacgatgt agaccatgtt
     5401 gatccgacgc ctgacgatgt gggcaaactg cccgagcccg atggaggccg aatccccatc
     5461 gcccgagatg ccgatgcaga tgagctcccg gttggccagg ttggcgcctg ttgccactga
     5521 cggcatgcgc ccgtgcacgg aattgaaacc gtgggatttg ctgaggaagt agctcggggt
     5581 cttggaggag cagccaatcc cggaaacctt ggcgaacttg tggggcggaa gggacagctc
     5641 cgcgcaggcc tgcacgacgg cagcgctcac ggagtcgtgg ccgcacccgg cgcagagcgt
     5701 cgaaaccggg ccttcgtagt cgcgtcgggt caacccgatc tcgttgacgg gcagtttcgg
     5761 gtgatgaacc ctcggcttga caacgaaagt cattacttga cctccttgag gcgcggcagc
     5821 cgctgctccg tgaagtaatc ggaaacctgc ttctggatcg tgtcggccga tatggaaaga
     5881 ccgccgtagt aaaggaccga gacgaggccg gcgggatcga gctcaccctc ggtcaccagc
     5941 agaaccttca tctgcgcatc gcgattctga tccacgacga aaaccgtatc gtgggccgcg
     6001 atgaaatcgt acacctccgc gccgaagggg aacgcggtga tgcgcatggt atccaggcgg
     6061 attccctcct cctccagcat gtccagggcc tcgcgcatgg gggccacgga ggttccgtag
     6121 tagataatgc ccgccctttc gccgttcgac tcgatctccg ccgaaggcac cagtctggcc
     6181 gccgttcgcc actttgcgct cagccgctcc atgttggcga cgtagacatc cggggattcc
     6241 gtgtacgcgg cgaactcgtc acgcgacgtg cccctggtga agaatgctcc tttgtccgga
     6301 tgggttccgg gaagcgtccg gtacccgatg ccgtccccgt ccacatccag gtaacggccg
     6361 aaccgctcca gcgaatccag ctgttcccga tccagcacct tgccccggtc gtagcgacgt
     6421 tcgtcatccc actcgaaagg ctcggagatg ctgtcgttca tcccgagctc cagatcactc
     6481 atgacgataa cgggcgtctg gagccggtcg gccaggtcga gcgccagcgc ggcatggtcg
     6541 aagcactccc tcggcgtcga gggaaacagc agcacgtgct tggtgtcccc gtgagaagcg
     6601 taggccgagg aaatcagatc cccctgctgc gtgcgggtgg gcatgcccgt ggacgggccg
     6661 gaacgctgga tatcgaacac gacggcggga atctcggcaa agtaggcaag cccgagaaac
     6721 tccgtcatca gcgagatgcc cgggccgctg gtcgcggtga aggaacgggc gccgttccac
     6781 gccgcaccga tgacgatgcc gatggccgac agctcgtcct ctgcctgaat gatcgcgaac
     6841 ttgcgctggc cggattggcc gtcgatacgc atccggttgg caaagcgctc gaactcctcc
     6901 gccaccgagg tcgatggcgt gatcgggtac caggcgcaca ccgtcgcgcc gccgtagagg
     6961 gctccgagcg cggcggccgt gttgccgtcc accatgatct tgtcgccgtt caagtcagcg
     7021 cgtttgacct ggaacggcaa cggacactcc agatagtccc gggcatagtc ccgaccgagc
     7081 tcgagcgcgt ggatgttcgg gccgatgagc gcgtctttgc ccttgtactg ggtggacacc
     7141 atgccggtga tcacgtcgaa ctcgatgttc aggagcgccg tcagcgcccc caggtaggtg
     7201 atgttcttga acaagctgcg ctgcttcggg tcgctgaact cgctgagcag catgctggat
     7261 atgggaatcc ccacctcgtg cacgtcgctg cgccgaagcg atgccggcag cgccttggta
     7321 ttgtcgtaca ccaggtaacc gccggggttc aggctcgcaa cgtcttcttc gaaggtctcg
     7381 gcattcatgg cgaccatgat gtccacacct tcgcggcgac ccaggtagcc acgctcgctc
     7441 accctgacct cgaaccaggt cggcaggccc tgaatgttcg acggaaatat gttccgcgtg
     7501 gcaaccggga tgcccatccg gaacagggcc tttgcaaaca tgccgttggc gctggccgag
     7561 cccgacccgt tgacgttggc gaaacgaatg acgaagtcgt tgactatggt gcgtttggtc
     7621 attctgcgta cgagcccagc tgcggaatgt ggatgatgga cttctgcatg tcccaggcgt
     7681 tcgtggggca gcgttccgca cagagcccgc agtgcacgca gacgtcctcg tctttcacca
     7741 tgacacgacc ggtgttgagc tcatcagata cgtagaggtc ctgctcgacg ttgccggccg
     7801 gcgcggtcag ccggcggcgc agatcgggct cctcggcgtt ttccgtgaag gtgatgcagt
     7861 ccatcgggca gatgtccacg cacgcgtcgc actcgatgca cagctggtcg acaaacacgg
     7921 tctggacgtc gcagttgagg cagcgttgcg cttcctttgc gccgagctcc tcgtcgaacc
     7981 cgagctcgac ctccatcttg atgtcggcca gcgccagacg cttgtccgca tggggcacca
     8041 ggtagcgccg ggccggatcg tgctcgctgt cgtagctcca ctcgtgaacg cccattttct
     8101 ggctgatgag gtttacgcct tccggcggac ggtcggcctt gagatccttg ccctggcaca
     8161 tgagatggat ggagatcgcc gccttgtgag catgggcaga ggcccagatg atgttctccg
     8221 gcccgaacgc cgagtcgccg ccaaagaaga ccttggggtg agtggactgc agcgtttcct
     8281 cgtccagcat ggggcaatcc catttgtcga actggatacc gatgtcccgc tcgatccagg
     8341 gacagtggtt ctcctggccg atggccatga ggatatggtc cgcctccatg aagacgtcgg
     8401 gctccccgct gggctcgtag cgcaggcgac cgtcgtcacc cttaaccggc ttgacgcact
     8461 cgaacaccac gcccttgagc ttgccgtttt cgtgaacgaa ctccttgggc ggccgattgt
     8521 tgatgatcgg tatgccctcg ctcatggcgt cttctttttc ccactccgag gccttcatca
     8581 cctcgaacgc ggaacgcacc actaccgaga ccgactcggc ccccaggcgc ttggccgtgc
     8641 ggcagcaatc catggcggtg ttgccgccgc ccatgacgat gaccttgccg gaaatcgagt
     8701 gggtgtgccc gaacgcgacg ctggagagcc aatcgatgcc gatggagata tgctcggcta
     8761 cttccgctct gcccggcagc tcgagatctc gaccccgagg cgcgccggta ccaatgaagt
     8821 aggcgtcgaa atcctcatcg agcatctctt tcatgctcga gacctcgtga ccgaagtggc
     8881 actcgacgcc catgtcgaga atcacatcga cctcttcttc gagcacgccc accgggaggc
     8941 gaaacgcggg tatctggctg cgcatcatgc ccccgcccat gggctctttc tcgaacagat
     9001 gcacctcgta gccgaaaggc aacagatctc tggccaccgc cagggaagca gggcccgcgc
     9061 cgagcagggc aatacgcttt ccgtttttgc gctccgggac cttcggcaga taggcgctga
     9121 tgtcgccctt gtggtccgcg gcgacgcgct tgaggcggca tatggcgaca ggtttttcct
     9181 cggtgcgcac ccgccggcac gccggttcgc agggacggtc gcaggtgcgg ccaagaattc
     9241 ctgggaacac gttggagtcc cagttgagca tgtaggcttc ggtgtatcgc tgctgcgcga
     9301 tcagccggat gtactccggc accggcgtgt gtgccgggca agcccactga cagtcaacta
     9361 ccttgtggaa atagttgggg tcgaggatgt ttgtaggttt catgcgtacg acgactcgcc
     9421 ggcaggcatg cacggctacg cctcccagct cctttggtta cgctaatttg cggcgacagg
     9481 gcaaaccgtt catccggccg actgctgggc catccatata gccaggccga gcctggacag
     9541 tcgcgaactc taaccttatt tttcgtggct tgccaaccaa attggccgcc tggtgtgaca
     9601 cgttgcccag cacgtctgag gccgcgtagc gaccagcgcc ggcgtcagcg acgacctcga
     9661 ggcgtcggaa gggggaaacc aaccggaacc gccaattccc gagtagaata cgcgccgcta
     9721 tggtcaaccg cccagaactc tgatggccaa cgagctgtcg ccattccaac cgccaacgcc
     9781 gccagccgtg ggttcccgca tcgcctggaa cgaacttcac gacggtgctg cggggcttgc
     9841 cgtaggccgc gctgccgccg cccacgaggg gctgacgctg atggtcgccg agagcacgcg
     9901 gcaggcccag cgctggctgg acgagcttcg attcttcgcc ccggacgtcc acctgctgca
     9961 ttttcccgac ggcgagaccc tggcttacga tgccttctcg ccgcaccagg acatcatctc
    10021 cgagcgtctg tccacgctgc gcgacctgcc gtcccagcgc accgaacggg agcccaccct
    10081 gctcgtcgtg cctgtccaga cccttctcca gcgggtagcc ccgcggacct acctcgacgg
    10141 ccacagcctt tcacttcgca cgggccagca cttcgacctg gatgcgcaac gcggccggct
    10201 caacgcggcg gggtacaacg cggcagagac ggtaagcgct cacggggagt acgccgtgcg
    10261 cggttcgctg atggacatct accccatggg agcggatctg cccatccgta tcgacctgtt
    10321 cgacgacgag gtggaaagcc tgcggacgtt cgaccccgac acccagcgca ccgtcgagaa
    10381 gatagacgcg attcggctgc tgcccgcaaa ggaatttccc ttggatggca aagccatcgc
    10441 acgattccgg agcaactggc acaacacgtt caacgtcgac gtgcggcgat gcagcgtcta
    10501 ccaggacatc agcgagggca tctccccgaa cggcatcgag tactacctgg ccttcttctt
    10561 cgaagagttg gctacgctct tcgactacct gccagccaac accgtggtca tccgggaggc
    10621 cggcgttcgg gaagccgccg agcggtttct tcacgacgtt ggcgttcgct acgagagcct
    10681 gcgtcacgac gtggagcgac ccattcttcc cccgtccgac gtctacctgc gcagagagga
    10741 gctcaacgcc gcgttgaagg cctggccgca gatcgtcatc aacgacgcca gcgcccgtca
    10801 cgcggtcgac ttcgcaactc aggcctgcgg agatctggaa gccaatcccc ggctgcggga
    10861 ccccgcgcgc ggcctgtgcg agtttctcga cggcatcacc cagccggtgc ttttcgtggc
    10921 cgactcggcc gggcgccgcg aggtgttcct ggaattcctg aacgcggccg gcatacgtcc
    10981 gcgagagacc gccgacctgc ccgcgttccg agattcgagg ctcgactgcg caatcgccgt
    11041 cgcccccgtg gagcagggtc tgtggaccga cgagctggtg atcgttaccg agagccaggt
    11101 tttcgggcat cagctcgagg agagacgcgc ccggagcgct cgcgtgctgg acccggaaca
    11161 gatcatcagg aacctaaccg agctgcacgt tggcgacccc gtggtccaca tcgagcatgg
    11221 catcggtcgc tatctcggcc tgaccaccct ggagatcgac ggcgcgccga gcgagtttct
    11281 cgccgtggcc tacgccgaag acgcgaagct ttacgtgccg gtcacatcgc tgcacctgat
    11341 cagccgctac gctggcgcag agcacgcgcc gctgcatcgc ctgggatcgg atgcctggga
    11401 caaggccaag cgcaaggcag cagagcgtgc ccacgatgtc gccgccgagc tcctgaacat
    11461 ctacgcccgc cgggccagcc actccagcta ccgcttcaac gacccgagcg ccgattaccg
    11521 ccgcttcgcg gcacagtttc ccttcgagct gacgcccgat cagggccgcg ccatcgacga
    11581 ggtcatcgcc gacctatgct ccgagcgggc gaccgaccgc ctcatctgcg gcgacgtcgg
    11641 cttcggcaag accgaggtgg ccatgcgcgc cgcctttctg gcggtgcaga gcaacaagca
    11701 ggtggccgtg ctagtgccca cgacgcttct ggcccagcag catttcgaga ccttccgcga
    11761 ccgcttcgcc gactggcctg tgcagatcga agcgctgtca cggcttcgct cggaaagcga
    11821 ggtcgaagcc atcgccgagc gaatggcgtc gggccggatc gacgtgatca tcggcaccca
    11881 caagctgctc aatcccgcgc ttagtttcaa cgatctgggt ctggtgatca tcgacgagga
    11941 gcaccgcttc ggcgtccgtc agaaggagcg gctcaaggct ttgcgagcag aggtcgacgt
    12001 attgaccctg acggccaccc ccattccccg cacgctgaac atggccatga gcggcatgcg
    12061 cgatctctcc atcatcgcca cgccgccggc caaacggctt tcaatcaaaa cgttcgtgca
    12121 ggaaaagcgc cagcacgtca tcaaggaagc cgtgacgcgc gagctgatgc gcggcggcca
    12181 ggtcttctac gtgcacaacg aagtgcgaac catcgaaagg gcggccgcag agctggcgga
    12241 gctggtcccc gaggccagaa ttggcgtcgg tcatggccag atgcccaagc gccagctcga
    12301 gcgcgtcatg aacgacttct accatcgcca gctgaacgtg ctcgtctgca cgaccatcat
    12361 cgagacgggc atcgacatcc cgaatgccaa caccatcatc atggagcgtg ccgacaagct
    12421 gggtctggcg cagatgcatc agcttcgcgg ccgggtcggc cgatcgaatc ggcaggcgta
    12481 cgcctacctg ctgacgccgc atcccaaggc gatcaccaag gacgccgtca aacgcctgga
    12541 agccatcgag gcggccggcg agctcggcgt cggtttcact ctggccaccc acgacatgga
    12601 aatccgaggg gcaggcgaac tgcttggaga ggagcagtcg ggacagatcg agtctgtggg
    12661 atattccctc tacatggacc ttctggaccg ggccgtaaaa gccattcagg atggcaagac
    12721 gccgaacctc aatgccccgc tggaacccat cgctcaggag gtcaacctgc acacgccgac
    12781 gctcatcccg gagcattacc tgcccgacgt gcacgcccgg ctgattctct acaagcgcat
    12841 cgccaacgcc gccgacccga cggagctaga cgatctgcag gcggagatga tcgatcgctt
    12901 cgggctcctg cccgagccgc tgaaatccct gtttgccgtc acggccttga agctgaaaac
    12961 ccagccgctg ggcgtcgttc gcgtggatct cggcgaaagc ggcgggcgga tcgagttcag
    13021 ccccgacacc gaggtcgatc cgctggccat cgtgaacctc gtacagagcc atcccgggac
    13081 gtaccggctg gacggcgcga cgatgctgag gatcaaccgg acaatgccgg actttcaggc
    13141 gcgaatcacg ttcgcaggcg agcttctaga tcgcctggcg gcacacacac cgaaccggct
    13201 ggtggccaac gcatgagcgc catgagcccc cgaagagcgg ccgccacggc gggccttgct
    13261 gccgcgctcc tcggacaggc atccgacgcc atggaggccg aagaacctgt cgatcttctg
    13321 ctcaaagact ggtacgtcac ggagatcatc gtcgtgcagc gccccccggt catggatttc
    13381 aactccgccg aacggctcct gcgggaccgt ccgccggcgt tgcccctggc tatgcgcacg
    13441 ttcgccaccg atccctccca gctcggaaaa gacctggatc tggatctgtc gacccgcctg
    13501 agcctcgtgc atccgaccct ggctttgcta ccccctgcgc aggacgaagc agacgtcgac
    13561 gagggatccg gggcgatggt tgctccagcc atcgaaccgg tgctcgaacc cgaccccctc
    13621 ctcgatgtgc tggccatggc cgccgcgctc gaggagcaac tcgaggcccg cagctttcag
    13681 ctcatcccgg ccgaggagct gaccatgtcc gccagcgccg accggctcgc ccggcgaagc
    13741 ggcttccagg tcctctatca cggcgcctgg ctgcaaccgg ttccgccgcg cgatgcgccc
    13801 gaatacctgc tgctgcagac cggcgccaga gtggccgacc gccaccagct cgaaggggtc
    13861 gtggccgtca ccctcgggcg gtacctgcac tttcaggcca gcttctctta caccgagccc
    13921 ggcatggggc tcgagccgat gactttggtg ctcaacccgg acggttcggg cacgccggcg
    13981 cgatggcccg ttgaaactcg cccggtgatg atcatgaacc agagtcgacg catgcgcagc
    14041 gaggaggttc attacctgga tcatccgaag ctgggcatca tcgtgcgcat cgatccggtg
    14101 cccgttccgg aagaactagt cgccgcgttc gatgccctgg aagaacccac cgagtaggcg
    14161 cgccacgtcc tccatgccct cacgcgcaat gaccgcgatg tggtccaggt cgatggcatc
    14221 tccccgaccg gcagccgggt tcaccaccag gcacaagctc gcgtaacgga ttcccagctc
    14281 ccgggcgagc ccggcttcgg gcattcccgt catgccgacc accgtgcaac cgtcccggtc
    14341 catccgatcg atctcagccg gcgactcgaa acgcgggccc tgagtgcacc catagacgcc
    14401 gccatcgtgc acgtccagcc cctgcccccg ggcagcagcc agcagctcgg ctctcagcgc
    14461 agcatcatat ggctccgtga aatccacgtg cttgaggtcg ccctctgcag cgaagctgtg
    14521 ggcgcggccc cacgtgtagt cgatgatctg atcgggcagc acgatgcgcc cgttgcgcgc
    14581 gtgcttggcg atcccgccga cggtattcag cgcgacgatc tcctcgacgc ccagctcgga
    14641 gagcaggtgc agattggccc gataattgat caggtgcggg gcgaccgcgt gcggaacccc
    14701 gtgtcgcgcc agcacatgga cgatgaagtt tcgaccggca gcgcgcgaag gcttggagga
    14761 ggcctcgccg tagggggtgt cgcggaagtc ctcgaccaac acatcgaatg gaagcttcag
    14821 cgcaccggag cccacgagta cagccagacg tctcatcaga ccgcttcatc ctcgagggat
    14881 ctcggcaacg cgtaaattgc cggtaggttt cgccagtagc cgcgaaaatc catgccgcaa
    14941 ccgaagagat agcggtccgg gcagcacagc gccgcgtaat cgacgcgggc cgctccgctg
    15001 cgcgggaccg ccttgtccac tagaacggcc gtggtgacct caacggcccc ttctcggcgc
    15061 agccactcca cgacctgcat gagggtctcg cccgcatcga aaatatcgtc caccagcagc
    15121 acgtggcgtc cggcgacgct cactgggggg cgggctagcc attccagctg ggacccgctg
    15181 gtacgctggc cgtagcgggt cgcgtgcagg taggagagct ccagcggaaa gctgaagcgc
    15241 ttgatgagct ctgcggtgaa cgggagcccg ccgttgagca ggcagagcac cagcgggttg
    15301 gcagcctcca gagcaacggc gaggcgcacg gcggtccgat caatcgcggc ctgcacctcc
    15361 tgcgctccga cgacgagctc ggccgcctcc cgcgcggtca gaatctcagt ggggacccga
    15421 gcagtcacga ctcgggctgc accacgtgca gcgtctgggt ggggaaggca atctccgcgc
    15481 cgtgctcttc gatgatgtcg gcgatgcgaa agagcacgtc ctgcttgatg gcgtgaaact
    15541 cggcccagac cgtggtcttg gtgaacgtgt agacgaagaa gtcgagggag gaaggcccga
    15601 actcgacgaa gttgaccatc agcgttctgc cgtggtcgat gtccggatgc tctcggagca
    15661 tggattccac gtcggccacg atctgccgca gcacgccgac gtcctcgtac cgcacgccga
    15721 tggtttcgta gatgcgccga ttgcgcatcc gcgacgggtt ttccaccgcc agattcgtga
    15781 atatcgagtt cggcacgtag agcggccgct gatcgaatgt ccggatccgg gtcagccgcc
    15841 agcctatgtc ctccaccgtg ccctcgatct cacggtcagg ggaacgtacc cagtcaccaa
    15901 cggagaacgg cctgtccagg aaaatcatga gcgcgccgaa aaagttggcc agcaggtccc
    15961 gcgcggcgaa acccaccgcg atgccgccga caccgccaaa ggcgaggacg ccagcaatgc
    16021 tgaacccgaa ctcctgcatg gccatcagaa cgcccgtgat gatggcggcg gcccgcagga
    16081 gctttcccac ggcggcagcg gtcgtttcgt ctaccggctc ctcccgataa gacgagtcgg
    16141 ccagatgcac ctccatgaat cgaatgaacc ggagggcgaa ccacacgagc agccagatga
    16201 tggcgacctc gcgcacctcg tccacgtagt cgaaaatctc ggccccgccc accgccccgg
    16261 caacctcggc ggcccaggaa acaccgagca cccagatccc gacgccaacc ggtccccgcg
    16321 cggcatcgaa cagggcgtcg tcgtagatat tgtgggtctg ctcaagacgc ccggcgagcc
    16381 gctcgaatgc ccggcgcacc gcgtagcgca ccagcgcggt ggcgaaaacg atggcgaaca
    16441 cctccacgac ccaggtgttc tgactgagaa aatcccgcaa cgattccagc atgaggccct
    16501 gccgcctgtc agaagtaccg ggtcagctcg agctgaagaa agtcgtcccg ctcgaggggc
    16561 caggcacggt tattcggatc cgtggagggc ggcacgcccg gcatcggcct ttcggagccc
    16621 ccgaagatgc gcccctccac cagcagggtc caagtgtcgc ccaggcggcg gctaccttcc
    16681 agcttgacca cgtattcgtc tgtcttgacg tcccatatca cgccgatcag cgcctgggtg
    16741 tcagccacgt cgttcatctg ccagcgggtg ccgagcgcca ggtcgtgctc gaagagcgta
    16801 tcgaaagctc gctcgtcgcg atcgtcgaac atgtactcga ccaccagacc caggtcgccg
    16861 cgtcccccga gaacgccgac cagagagcgc tcgaagccgg cgttgaacgc cgagtagcga
    16921 ccttcgtaac cgctgctgga aaccgcctcg agcttccagg cccaatctcc gacgatggcc
    16981 tgagcatcca gaccggtctg atcgatcacc ggatagaacg ggcgcagcac ggcctgcccg
    17041 gccgggccgg tctcgggaat cagcacgggg gcgcggctcg tccccgagaa atgatgaatc
    17101 ccgagggtca accgccccag ctgacgctgc cagcgaacgg cgccgtcgac ccgccagcgc
    17161 ccggcgccgg actcgtagcg ggcatcgtca tccaccggca agggtggccg cagacgcccg
    17221 tcggagccgg gaaaagtccg ctcgcgaaag ccggtcagca ggtaaaagtc caggatgccc
    17281 cagtcgcgca cgaccgagag gtgggccatg ggctgtccca gcttgtcttc gccgtcgatg
    17341 ttctctacca ggtcggtctg gttgatcacg tcgaccaggt gtttgaattc cgtgacgccc
    17401 cagaaaacct gcttagctcc cagatgaagg tcccagtccg gacccacgcg gctccagaac
    17461 agttcccgca cgtcgaaatg cgtgcgctcg tcgtcctcgc catcgaccct gacgaacggc
    17521 gtgaacgtca ggctggatcg atcctcgttc caggcacggt agtactccag ctgagcgctg
    17581 accgatccct gggtccggga ctggccatcg aggccgctct tcgagaaagc ccgcagctga
    17641 agtcccaggt cgaggtccag cgagtgatca gcggaggacg gctcgatgcc gtcggtagag
    17701 gctggctgtg cggccaaact gatcccgccg gcagcgagca gcgcagctgc caggcaccag
    17761 ggacggggcc cttggcaagt catcgcaccc gcagcaggct gttggtagaa aaatcgcgct
    17821 cgtccatgag ccccgttcgg aaccggtagt tgcgccagaa gagctcggtg ctccggtccg
    17881 tctgatggtt ggtcatgtac atccggccgg ccttccagaa ctgatcctcg taacgggcat
    17941 actgatcgat ggtcagggtc ttgagcaggc tgtcccgccg gtcgaagtac tcgatccgca
    18001 gcacgcgcag ctcgtcctga tcgatccaga cctgctgccg cgagtagccc gagaagtcgt
    18061 cgcgcggggt acgctccacc acgaagcagt cgatcccgtt agcctgcccc tggccaagga
    18121 agcggtagct gaacttttcg atctcctgaa cggtaaggtc ctcgtaggcg aactcgctgc
    18181 tcagaaacgg tcccgacttg ttctggctgg cgatcttctt gactcgtttc atggcaggc
//
LOCUS       DBNHKV010000401        34765 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1189715, whole genome shotgun sequence.
ACCESSION   DBNHKV010000401 DBNHKV010000000
VERSION     DBNHKV010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 34765)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 34765)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34765
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1189715"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="AC16PP_12885"
     CDS             <1..847
                     /locus_tag="AC16PP_12885"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR6482569.1"
                     /translation="VLLENVRFLVGEKTDDPRLAKALGALCDIYVMDAFATAHRAQAS
                     THGVAKFAPVACAGPLLAGELEALERALRAPARPMTAIVGGAKVSTKLEVLDTLATIA
                     DRIIVGGGIANTFIAAAGHPVGRSLYEEDLLEAARSIAAKVEVPLPVDVMVGERLDAG
                     AVAVLRGLDEVRPDEMILDIGPETARRYAAELGSAGTILWNGPLGVFEFDQFGEGTRV
                     VAQAIASSGAFSIAGGGDTLAAIDKYDVRDGVSYISTGGGAFLEFVEGKTLPAVAVLA
                     SRAEG"
     gene            861..1820
                     /locus_tag="AC16PP_12890"
     CDS             861..1820
                     /locus_tag="AC16PP_12890"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6482570.1"
                     /translation="MEVGFDPDSLRQALKPLVPGSPTVLTPQEDAYLRHYGIHFVEEY
                     PDLAHHFGAVETDSHSVAAHLWSPRDARGTAVIVHGYYDHTGLYGHLIRHLIDRGLAV
                     LSFDLPGHGLSTGAPATIETFDHYVAAFDACIAALGAHLPKPWHLLGQSTGGAVAMEW
                     LLANGVARATSPFSGIVLLAPLVRPYLWPVGRLFYEVVRRFITERERTFSVNAENPEF
                     IEFLKHHDPLQARVLPVQWVTAMVAWKKRFVSRAPSDLGLLVVQGTADRTVDHRYNLK
                     IIEQLFDAKVVYVPEARHHLVNESVVLRQQIFQAVDAQLDAAG"
     gene            1828..3204
                     /locus_tag="AC16PP_12895"
     CDS             1828..3204
                     /locus_tag="AC16PP_12895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013974677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6482571.1"
                     /translation="MTSAHGQLTSALSPSQVRTDPDSLHEWGRDWTRTAEPAPAAVVF
                     PETTADVVAIVRLANSEGLKLVPSGGRTGLSGGAVAADGEVVVSFDRMNRVLEFSAAD
                     RLLSCQAGVVTRTVQDFARERGLSYPVDFASSGSSQIGGNIATNAGGIKVIRYGLTRD
                     WIAGLRVVTGAGELLDCNRGLVKNATGYDLRHLFVGSEGTLGFIVEADVRLAPAPAPQ
                     TVMLLGVTEFPRLIDVLSVLQSTVVLSAFEFFSELALQKVLSHRKLRRPLAETAPFYA
                     LVEFDTADLEPATAAYEAAVGGGWVCDGVVSQSEGQAAALWSLREGISESIAQFTPYK
                     NDLSVRVSVLPEFLAQVDGLVAQSYPGFEVCWYGHIGDGNLHLNILKPGGLSLAEFYA
                     RCHAISPSLFELVARHGGSVSAEHGVGLLKRDFIGYSRSAAEIEIMRALKGVLDPNQI
                     MNPGKLLS"
     gene            complement(3239..3904)
                     /gene="rpiA"
                     /locus_tag="AC16PP_12900"
     CDS             complement(3239..3904)
                     /gene="rpiA"
                     /locus_tag="AC16PP_12900"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HIR6482572.1"
                     /translation="MDQDQLKRSAAEAALAYIEPKLGSASIVGVGTGSTTNHFIDALG
                     AIRHKFDAAVASSEASAARLKAVGIPVIDLNAAAAVAVYVDGADEVNAARQLIKGGGG
                     ALTREKIVAASADEFVCIVDESKVVQTLGAFPLPVEVIPMARGLAARAIVGLGGAPEL
                     REGFVTDNGNIILDVYELTITDPAAMESAVNNIVGVVCNGVFAAQAADLVIVAGASGV
                     TSF"
     gene            3977..5488
                     /gene="ilvA"
                     /locus_tag="AC16PP_12905"
     CDS             3977..5488
                     /gene="ilvA"
                     /locus_tag="AC16PP_12905"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HIR6482573.1"
                     /translation="MEKYVKKILSSRVYEAAVETPLQPARNLSIRLGNHVRLKREDMQ
                     PVFSFKIRGAYNRIVQLTEEERARGVIAASAGNHAQGVAYSAQQLGIKALIVMGRNTP
                     SIKVNAVRALGAKVVLHGDAYDEAAAHALKLAERNGYVFVHPYDDPDVIAGQGTVGVE
                     ILNQHVAVPDAIFVPVGGGGLIAGIAAYVKYLAPDTRIIGVEAEGSACLAAAMAAGRR
                     VRLPFESLDLFADGVSVAQIGKETFKVARRCVDEVITANTDEICAAIKDLFDDTRSIA
                     EPAGALAVAGLKKFVEREGVRNLNLVAVVSGANVNFDRLRHISERAEVGEHREVVLGV
                     TIPEKPGSFLKFCKMLGNRPVSEFNYRFATDREAQVYVGLQVSAADDRAELMKSLSTG
                     YVVHDMTDNEAAKLHVRHMVGGRTFGSKPELLYRFEFPERPGALLRFLTGLGSRWNIT
                     MFHYRNHGAAWGRVLVGFEAQPRDRRSLGEYLKRIGYRYWEETDNPAYQLFLR"
     gene            complement(5495..6028)
                     /gene="def"
                     /locus_tag="AC16PP_12910"
     CDS             complement(5495..6028)
                     /gene="def"
                     /locus_tag="AC16PP_12910"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR6482574.1"
                     /translation="MAVRKIIRMGHPTLRRVARPLAGEEIRSEAVGRLIGDMIDTLHD
                     YGGIGLAAPQINESVRLAIIEIPGGPTRYGEIPALPLTVFINPSVEVVDPATAGYWEG
                     CLSVPGLRGFVERPQHVRVNALDQEGKPLDLELKGFLATVFQHEFDHLDGRLYVDHLK
                     DPALLAFDEEFERYLAE"
     gene            complement(6048..6632)
                     /locus_tag="AC16PP_12915"
     CDS             complement(6048..6632)
                     /locus_tag="AC16PP_12915"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR6482575.1"
                     /translation="MTSTLERQELRRFFRERRRALGSHQQREHALRVARHLLNNPVSW
                     RSRRIGAYWANDGEVSLAPLLGELEYRNRQLALPVVSTAGRMAFYRYHRAAPMLVNRY
                     GIPEPGPGSAFVDGRSLDLILLPLVAFDEYGVRLGMGAGYYDRFLGALPEPLRPRIVG
                     IAHEVQRSADPLPFQDWDIPLDGIVTERGWQAFT"
     gene            complement(6654..6864)
                     /gene="ssrS"
                     /locus_tag="AC16PP_12920"
     ncRNA           complement(6654..6864)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC16PP_12920"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(6937..7251)
                     /locus_tag="AC16PP_12925"
     CDS             complement(6937..7251)
                     /locus_tag="AC16PP_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR6482576.1"
                     /translation="MSEEATTVSVKILDKEYQVSCPAEEVDALSRSARYLDEQMRSIR
                     ESGKIFGMDRIAVMAALNIANEFINSSKSLESTHGVVSQKVTELTERVGRALSEQKQL
                     NL"
     gene            complement(7265..7471)
                     /locus_tag="AC16PP_12930"
     CDS             complement(7265..7471)
                     /locus_tag="AC16PP_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HIR6482577.1"
                     /translation="MADTEWTALESKVDELIELCSVLTKENRALRAQQQNWTTERAKL
                     IEKNELAKARVESMITRLKALEQD"
     gene            7593..8135
                     /locus_tag="AC16PP_12935"
     CDS             7593..8135
                     /locus_tag="AC16PP_12935"
                     /inference="COORDINATES: protein motif:HMM:NF015642.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HIR6482578.1"
                     /translation="MADLTALALGAVESLSVAEIHGAVCGLAVASEGGIELGSLVELV
                     GIEALRDEHSVGEFVNAAVDALHAEDLSFAPLLPDDHVDLALRLEALGEWCGAFLAGF
                     ASGSRFQELGEEQIPEEAREIIGDFTYIANVDGSEAGDGEDPESAEKDYAEVHESVKV
                     GALLLRTLLGGDAPEETARP"
     gene            8132..9460
                     /gene="pepP"
                     /locus_tag="AC16PP_12940"
     CDS             8132..9460
                     /gene="pepP"
                     /locus_tag="AC16PP_12940"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011536293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HIR6482579.1"
                     /translation="MIAAAEYRRRRQRLMSLMDEGSIAVLPAAGQRFRNRNTEYPFRQ
                     DSDFYYLTGFAEPDAVLVLAPGRAHGQVILFCREREARAERYDGERVGPERAAERIGV
                     DDAFPVNDLEEILPGMLEGRERIYITLGEYPEFDNRLMHWVAGIRAREAGGAIPPGEF
                     VALKHLLHEQRLYKSAAELRAMREAARITCSAHRRAMAACRPGMTETQLEAELLHEFM
                     VNGARSPAYPSIVGAGANACVLHYVDNHDVLKKGDLVLIDAGCEYQHYASDVTRTFPV
                     AGKFSAAQRALYEVVLEANRQGIEACRPGATFIEPHSTALKVMVEGLIELNLIQGDVE
                     EILQSERYREFCPHNSSHWLGSDVHDVGDYRVEGAWRPLEPGMVLTVEPGIYIPADDS
                     TRHLAPRWRGVGIRVEDDVAITRQGHEVLSASAPKSVDDIERLMRGGADG"
     gene            9453..10634
                     /locus_tag="AC16PP_12945"
     CDS             9453..10634
                     /locus_tag="AC16PP_12945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6482580.1"
                     /translation="MADVVVVGGGLIGALAALGVADGGRRVTVLERREPERQRGRLGV
                     DIRNVAVSPASRELLEAVGVWSSLEPAAYQRMEVWEERGTRGMHFDAAEVGRAELGWI
                     LESSQVVRSLWDLLQRHRNVRLEVGADLASLRLAASGAVLETDRGDIRARLVVAADGA
                     RSRVRELLAVPAEEAPTGHHALATVVRTEQEHEGTCLQRFLLEGPLALLPTADAHLSS
                     VVWSQPAEEAERRAKEPDGDFCLELERCLEARFGKVEAVDARVVFPLAQTLVSTFNPH
                     HRVLLIGDAARVVHPLAGMGANIGFEDVRDLLAELAGIGGDQDPGAPGIWRRFARQRR
                     LRSQMVLALMSGFRRAYSADGPLASWVRNTGVGWLDRAQPLKAQIMREALGVGPLARR
                     W"
     gene            10723..11790
                     /gene="gcvT"
                     /locus_tag="AC16PP_12950"
     CDS             10723..11790
                     /gene="gcvT"
                     /locus_tag="AC16PP_12950"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017530883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR6482581.1"
                     /translation="MTRRTPLYELHVGLGAKMVDFAGWEMPLHYGSQLDEHHAVRRHV
                     GLFDVSHMTIVDVSGARSLDFLQRLVANDVARLEAPGQALYGVLLNPAGGVIDDLIVY
                     RRDADFRLVVNAATREKVLNWLAGSNEEAVNILERDLAMLAIQGPAAVEMYQSLPDSL
                     EVRGLKAFTAAVRGEEMIARTGYTGEDGLEIMAPGDRARQLWLELIGSGAQPAGLGAR
                     DTLRLEAGLNLYGQDMDDETSPLVSNLAWTIAWEPSGRNFIGRQALEDERARGIAERL
                     TGIVLEDKGVIRRGCRVVTAAGDGHVTSGIFSPTLGYSIGLARVPKGAKGPCEVEMRG
                     RLKQARIVRPPFVRNGLQVFE"
     gene            11787..12182
                     /gene="gcvH"
                     /locus_tag="AC16PP_12955"
     CDS             11787..12182
                     /gene="gcvH"
                     /locus_tag="AC16PP_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR6482582.1"
                     /translation="MSDVPQELKYARTHEWARLEEDGTITVGISDHAQDALGDVVYVE
                     HPEPGQPVAAGDEAGVVESVKAASDIYAPVSGSVIAVNEALDDAPETVNQDPYGDGWF
                     FKIQPDDVAELDELLDSEAYAELCENEEH"
     gene            12214..13572
                     /gene="gcvPA"
                     /locus_tag="AC16PP_12960"
     CDS             12214..13572
                     /gene="gcvPA"
                     /locus_tag="AC16PP_12960"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HIR6482583.1"
                     /translation="MPFIPHTAEEVQVMLSQIGVARTEDLFDEIPQNLRAGALSSVPA
                     GASELEMLRKLGARAALDESVVCFAGAGSYDHHIPAAVWDLTSRGEFMTAYTPYQAEA
                     SQGTLQLIYEYQSMMTALTGLDVSNASVYDGASGLAEAVLMAVRSNKKARSRTVLLPD
                     SLHPHYAAAARNICRHQSVEFVGMALDHDGRVDLEALETDGPDEVCAVVVQQPNFFGM
                     LEDVDALTDWAAERGGLVIAVVNPLALAVLKPPGEWGGSGADIACGEGQPLGVPMSSG
                     GPAFGFICCRSALVRQLPGRVIGRTEDLTGKPGFTLTLQAREQHIRRGKATSNICTNQ
                     GLLVTAATIHMSLLGPLGLARIAEHCHANTRALVEALTAIDGVEARHQGPFFHERVLQ
                     LPRPADEVVAGLLERGILGGVALGAYHESMNHDLLVCATEKRTDDEIGRYAAALREVI
                     AR"
     gene            13569..15032
                     /gene="gcvPB"
                     /locus_tag="AC16PP_12965"
     CDS             13569..15032
                     /gene="gcvPB"
                     /locus_tag="AC16PP_12965"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HIR6482584.1"
                     /translation="MSDVRTIFELSAQNRGAAAQHLSERPGAMDDIPASLRRERAPGL
                     PEVSELQVVRHYTNLSRKNFSIDTNFYPLGSCTMKYNPRGAHRAASLPGFLNRHPLAP
                     DSASQGFMACLHELQEILKDVTGMQGVSLTPMAGAQGEFAGVAMIRAYHEARGDHARN
                     EILVPSAAHGTNPATAAMCGYRVAEVPVNASGDVDLEALKLAVGPQTAGLMMTNPSTC
                     GVFERGIESIAAVVHDAGGLLYYDGANLNAILGKVKPGSMGFDVLHMNLHKTFATPHG
                     GGGPGAGPVGVGERLLPYLPVPFVAQDAKGAYRWVTDAELPDSIGRLSAYAGNAGILL
                     RALAYALMLGREGMHRVGEYSTLNANYLARRLEDAGFELAYPDRRATHEFILTFSESA
                     RSRDVTAMDFAKRLLDYGIHSPTTYFPLLIPECFLIEPTETETKAELDAFVDALVAIQ
                     REAEADPEKVKGAPYDLPVRRLDDVKAARELDLAWRG"
     gene            complement(15029..17104)
                     /locus_tag="AC16PP_12970"
     CDS             complement(15029..17104)
                     /locus_tag="AC16PP_12970"
                     /inference="COORDINATES: protein motif:HMM:NF015168.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR6482585.1"
                     /translation="MSNRLADETSPYLRQHADNPVDWHPWDEAALALASEQQKPILLS
                     IGYSACHWCHVMAHESFEDPATARVMNEHFVNIKVDREERPDLDKVYQLAHQLLTQQG
                     GGWPLTMFLDPDTRLPFFGGTYFPKTPRYQLPGFVDLLLRIAETFASQRDELRDHGTR
                     VADVLGRLAGGDSQAGELGDAELLAAAREQLGAQADEDEGGFGGAPKFPMPETVTYLL
                     RHWAYAQRSGEADRQGLEFVMTTLTRMARGGIYDHLGGGFCRYATDRRWMIPHFEKML
                     YDNGQLLALYSDALAIGPDELFSQAVSDTADWLLREMRHPQGGFYAALDADSEGEEGK
                     FYLWRRDQVKRLLDEDEYLVVETLYGLDKPANFEGKWNLHRYDAWRSVVERLSMDRAA
                     ANDLLASARRKMLAARELRVRPGLDDKVLTAWNGLAIKGLARAGSRLGQSRWLDAAAQ
                     AADFARARLWRDGMLYATWQGAPRYPAYLDDYANLLDGLIALLGARWREEDAAFACKL
                     ADGALERFYDADRGGFFFTAHQHETLIHRPKPTMDDALPPGNGVMVQALTALGHLFGE
                     TRYLEAAAATLAWARAAMEQYPAGHCCLLSGLQDEIMPPELVVVRGAAATFPDWRRAA
                     VSGYTPWRAVYCIPFEGVSTLPAYLPGPVSDDPHSQTVAYVCSGLSCSLPIGKPEDLG
                     EALRSRPRV"
     gene            17246..17743
                     /locus_tag="AC16PP_12975"
     CDS             17246..17743
                     /locus_tag="AC16PP_12975"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HIR6482586.1"
                     /translation="MVDADGFRPNVGIVLSNSIGQVLWARRVGGQDAWQFPQGGIREN
                     ESVQAALFRELFEEVGLVADDVEVLASTRGWLRYRLPRRMRRYNSSPGFQGQKQKWFL
                     LRMLGDDARVQMDGSAKPEFDSWRWVSYWYPLDQVVSFKRDVYRAALKELAPHLPVED
                     VASPC"
     gene            17737..20022
                     /gene="ptsP"
                     /locus_tag="AC16PP_12980"
     CDS             17737..20022
                     /gene="ptsP"
                     /locus_tag="AC16PP_12980"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009396724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR6482587.1"
                     /translation="MLTTLRRIVHGVEESSSFKEALSLLVGEVREALGTEVCSAYLLN
                     PEGDSYVLVANEGLNTDAVDKLSLGLNQGLVTLVAERAEPINLSDATHHPRFYLVPEI
                     GEEAFNAFLGVPVIANRRVLGVLVVQERDARRFDESEEAFLVTLAAQLATVIAHHEAT
                     GEIQQLLEDSSVEPIAREDALYKGVPGAPGISLGTAVVMHPTASLEAVPERIAEDVRT
                     ELMVLDRAVNAVRDDIRRISEQFAQSLPPEELALFDAYLHMLDDNALAGDVRDRINRG
                     QWAQGALKQVIREHVRRFEAMEDPYLRERGADVRDLGMRVLAYLQDIRSRKTYFPDDT
                     ILVGHEITPSMLANIPPEKLKGIVSVRGSSNSHVAILARALGIPAVMGAMDLPIFSME
                     NISVVVDGFYGEVFTNPSLALAGQYLQLIAEEREFAEELEDLRELPSVTKDGWPVGLW
                     VNIGLTGDITRSLDRGAEGIGLFRTEVPFMEKERFPSEEEQRAIYREHMEAFDPRPVT
                     MRTLDVGGDKSLPYFPIHEDNPFLGWRGIRVTLDHPEIFLVQVRAMIKANAGLECLLR
                     IMLPMVSSLSEVEEAKALVERAYVEVIEEGIVVKQPLVGVMIEVPAAVYQARQLARMV
                     DFLAVGSNDLTQYMLAVDRNNPRVAQLYKEMHPAVLQALREVARAAHAEEKVVGICGE
                     LAGTPAGAVLLMAMGYDALSMNATNLPKVKWTLRNVKRSDARRMLVNVLHMDTAEEIQ
                     QFMQDWLIDSGLGRVVPSYHH"
     gene            complement(20031..20777)
                     /locus_tag="AC16PP_12985"
     CDS             complement(20031..20777)
                     /locus_tag="AC16PP_12985"
                     /inference="COORDINATES: protein motif:HMM:NF017551.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007030 - Golgi organization [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HIR6482588.1"
                     /translation="MCLVLIAYQPESPFPLVVAANRDELYARPARSAHYWADRPHLLA
                     GRDDSAGGTWLGVTRGGRFATVTNFAEEVAEDAPRSRGELPTAFLDSDVSAHDYAHHL
                     DGPSYRGFNLLVWDGDALVYTSNRGTTQDLAPGIYGLANAELGATWPKVTSGVGALRR
                     TLSSGPTVDDLLGLLQDDTQPPDSELPSRGRTIELERQVAPRFIRGDDYGTRASTAVI
                     IGADGIRFAEQEFGPQGVPGRRSDFEVARA"
     gene            20860..21693
                     /gene="lgt"
                     /locus_tag="AC16PP_12990"
     CDS             20860..21693
                     /gene="lgt"
                     /locus_tag="AC16PP_12990"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019485279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR6482589.1"
                     /translation="MHYPDIDPVIFAIGPLAIRWYGVAYLCAFAMCWWLGVVRAPAKW
                     WTREEVADIVFYGAVGAVLGGRVGYVLFYGFEELIRDPLFLIRIWDGGMSFHGGLLGT
                     LIALWWFGNRTHRSFLQVSDFVAPLVPVGLGFGRLGNFANTELPGRATDSVFGVIYPC
                     SAESIRSINPLCTGQWEAFARHPSPLYQAFAEGVVLFALVWLVSGRSSRTGVVSGTFL
                     IGYGVLRFVTEFFRQPDAHLQFEALEWLTMGQRLSIPMVIIGILLLFWPRLRGGGETR
                     G"
     gene            21800..22594
                     /locus_tag="AC16PP_12995"
     CDS             21800..22594
                     /locus_tag="AC16PP_12995"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR6482590.1"
                     /translation="MKQYLDLMRQVRDTGVFKDDRTGTGTYSIFGHQMRFDLSESFPL
                     VTTKKVVLRLIIHELLWFLQGSTNIKYLTDHNVHIWDEWADEEGNLGPVYGSQWRSWP
                     TPDGGTIDQIAAVVQSLRTNPNSRRHIVSAWNVAEVDSMALPPCHTMFQFYVAQGRLS
                     CQLYQRSADIFLGVPFNIASYALLTMMIAQVCDLQPGEFIHTFGDAHLYANHLEQADE
                     QLSRVPLPLPTVTLNPEVRDIFGFRYEDFTLTNYRCHGTIKAPIAV"
     gene            22601..23104
                     /locus_tag="AC16PP_13000"
     CDS             22601..23104
                     /locus_tag="AC16PP_13000"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR6482591.1"
                     /translation="MHVALIWAMARNRVIGRDNRLPWRLPRDMQFFVATTMGKPVIMG
                     RRTFESMKAPLAGRTNIVVTSDTGYRREGVLVAHSLEAALDLAQAQCRADDVEEAMVI
                     GGAEVYRVALPIATRLYVTLIDADVEGDTFFPEIDLDAWREVSREAFPADAGNGLPFS
                     IVVFERP"
     gene            23117..25096
                     /locus_tag="AC16PP_13005"
     CDS             23117..25096
                     /locus_tag="AC16PP_13005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR6482592.1"
                     /translation="MTPRRIKGLCSPLGWILLVLIAGCARAPAPVPEPEAGRPGNVVV
                     SGVPAIPAGAAHGLMRYRNVRSAYLMGWADDGLVIATRFGETTQLHRLKTPLGAREQI
                     TFFSEPVLAAFLPTGAGALGAPGFVLSRDAGGSEHYQLFLFDWSTRHARMVSNGRARY
                     TNVLWSNRGDQYAYTTTERDGRRWDIHLQDLAGGIKVALETSDGGWRAVDWSPGDDRL
                     LLIKYVSATESYLYELELASGSLRPLLDESLAVAIGGARYAGNGESVYFTADLGAEFM
                     RLHLLELATNSVEVLTADVPWDVTEIAVTSDGAHLAMVTNEGGVSRLGVLRLPDHSPL
                     ALPEVPAGTIYGLRLSPDGRQLGFTVNAPTAPGDVYSIDLVGRTLTRWTRSEVGGLDP
                     SAFAAPSLVHYPTFDREGGARRKIPAFVYMPDGPGPHPVVIHLHGGPHSQYRPKFSAS
                     AQYYVKELGLAVVAPNFRGSSGYGKNYLKLDDGYLREDAVRDVGALLDWIAAQPRLDA
                     DRVAVSGGSYGGYMALASLIRYGDRLVAGVSRVGISNFVTFLENTRAYRRDLRRVEYG
                     DERDPQMRAFLEGISPLGQADRIDSPLLVIHGFNDPRVPASESEQIADAVSAAGTPVW
                     YVLAMDEGHGFRKKANSDYAAAVTALFLQRYLLDD"
     gene            25119..26129
                     /locus_tag="AC16PP_13010"
     CDS             25119..26129
                     /locus_tag="AC16PP_13010"
                     /inference="COORDINATES: protein motif:HMM:NF013663.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR6482593.1"
                     /translation="MAICVNPMSGRDVRRLAARASNMTHEAKRDMVARVAAGAQAMGA
                     SDIYVTREPFRIASLALEHMGLAAAVHVLQTPLTNGPADTEAAVAAFLAAGCRVIVSL
                     GGDGTNRAIARALGDAGAAGAGVDLIPLSTGTNNVFPVLAEPTAAGMVAGLIASGRLP
                     DDDLRQTAKVIHLSGTDARGRAVRDLALVDAVLLRDDHVGNLLPFAAQRLARIVLTRA
                     EPDAVGMSPIGGMLDVVEAGDDEGLLVEMGPGREFSAPLSPGAFQAVSVRSASRLPLD
                     VTVPLAGPGVIALDGDRDYKIAPREALTARIRRDGPRVVDIAAAMRRAVARGIMAPAR
                     NG"
     gene            26134..27327
                     /gene="fabV"
                     /locus_tag="AC16PP_13015"
     CDS             26134..27327
                     /gene="fabV"
                     /locus_tag="AC16PP_13015"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019820659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabV"
                     /protein_id="HIR6482594.1"
                     /translation="MIITPRIRGFICTTAHPAGCAAHVKEQIDYVSGQAALVGGPVVR
                     NALVIGASGGYGLASRIVAAFGCGAATLGVSLEKMPSETRTASAGWYNNVAFEEAAAA
                     AGLYAKTLDGDAFSDEMKGRVIDTLKADMGPIDLLIYSLASPVRQHPRTGELHRSVIK
                     PLGQALHMKSVHVDRGEVVEVDLEPADPEETAATVAVMGGEDWEFWIDALKDAGLLAP
                     GFRTLAFTYIGTELTWPIYWEGTLGKAKEDLDRAAGAISERLEDLEGNARVAVLKAVV
                     TQAMSAIPVVPLYASLLFKVMKERGVHEDPIQHIHRMFATQLCTGGALRLDDAGRIRM
                     DDLEMTDAVQEDVRDRWERVTTENLSELGDLDGFREEFLKIFGFGIEGVDYDADLDPT
                     LGRAP"
     gene            27424..28671
                     /locus_tag="AC16PP_13020"
     CDS             27424..28671
                     /locus_tag="AC16PP_13020"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR6482595.1"
                     /translation="MALRNLHLRAIPFRYAGYSGLALTILALQGTLRPAWALGVGAAG
                     CLIWTIAAHGAGRLAQTRGTSTVPFALGLHLAEALLAGALIGWLSLPWLPLLVAITVL
                     LASNAAQAGWWLAWRAGIAVAVGIGAGRLLAVAPVMSSTPAADLTASVLLLGYTVGLG
                     LTSFAKAQRLHAAQQDLARRALVTERLNSRLGRYLPPPVRARVRRRPDERCGLERRWL
                     TIAYVDVVGFTELAGRLAPEELAVVVNDYFAASGRLFASSGGTLAGLQGDGLLAYFGN
                     ATAATRGRAAVDCVESCLQVSRLLRELSECWQRRGYVTTLTTRCGVASGYCTVGDWGE
                     ERLDFTVIGAPVNLASRLQAHAGHDGILICEPTAALVGQVFPLGAERELVLRGIGKVK
                     ARRLESALVDPPPASAKVPRPEV"
     gene            28677..29717
                     /gene="hemE"
                     /locus_tag="AC16PP_13025"
     CDS             28677..29717
                     /gene="hemE"
                     /locus_tag="AC16PP_13025"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HIR6482596.1"
                     /translation="MTKTFLQACRGEATPHTPIWLNRQAGRYMPEYHQVKGDMPSLDF
                     FKNPERAARATLDAQRILGVDAAILFADLLPILEPMGLRLDYQPGVGPVFDNPIRSTA
                     DVDGLVSLPAEEATPYIAETVTNILEELPGEVALIGFAGAPFTLASYAIEGRGSRNYV
                     FVKKLMYEHEAVWHRLLDKLVEQVASYLHLQIRAGVDAVQLFDTWVGCLSVADFRRYV
                     LPHTRRLIQAIKEGSGGRVPVIYFGTGNSHLLADVVGAGPDVLALDWRVPLGETWKAL
                     GCTSIQGNLDPIVLCADRETVAEQAQVVLDQAAGRNGHIFNLGHGIIPETPVDNVKFL
                     VDFVHERSARSA"
     gene            29692..30120
                     /locus_tag="AC16PP_13030"
     CDS             29692..30120
                     /locus_tag="AC16PP_13030"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR6482597.1"
                     /translation="MSDRRVAPERIAAGLLLAAFWLPLAVCTYLALHPSPPDAVFRLS
                     DVLLHGFAFTYLTLALALAHGHRHRLLPGLWMLGYGVLIELLQSFETSRSAEVGDLLV
                     DVAGIGLGLLAYGWAGAFLRALAVRIASLVVGAGASNADS"
     gene            complement(30166..30615)
                     /locus_tag="AC16PP_13035"
     CDS             complement(30166..30615)
                     /locus_tag="AC16PP_13035"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR6482598.1"
                     /translation="MLDKGDTAPDFELPDQAGEPIKLATLLDSGPVVLYFYPADFTAV
                     CTREACAFRDRHEDLSNLDVRIVGISPQNQASHRRFAETYGLPFPLLCDTSKKVIRAY
                     GVDGPLGLGVRRVTYLIGRDRIIANRAVSDIFLGSHLALIEEVLGQE"
     gene            complement(30628..31206)
                     /locus_tag="AC16PP_13040"
     CDS             complement(30628..31206)
                     /locus_tag="AC16PP_13040"
                     /inference="COORDINATES: protein motif:HMM:NF047247.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HIR6482599.1"
                     /translation="MRLPYLLLLALITAMSFPGVGAEPPPAATEFPVPVVEAAGDFNG
                     VLARTAQVYIGGQPDASALTRMRALGVDTIVNLRTAAEMTNRQQVPFDEKAKAESLGM
                     RYVHIPAGGPKSPYSPSAVDQLAAAIAGTEGKVLLHCASGRRASHLWVAYLVRHEGLT
                     LEEAMSHGRAVNFGELPLEGFLGEPISFELAR"
     gene            complement(31206..31508)
                     /locus_tag="AC16PP_13045"
     CDS             complement(31206..31508)
                     /locus_tag="AC16PP_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482600.1"
                     /translation="MEIELKNGRVIHARPDATGSCAILTGPAPSEPRRWRDLTTDLPF
                     AFGRDAVISLAEPIEGEQTIVGLRVVFADGAELVYDVSGGRPRLTTSTDEISGARP"
     gene            complement(31607..31918)
                     /locus_tag="AC16PP_13050"
     CDS             complement(31607..31918)
                     /locus_tag="AC16PP_13050"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6482601.1"
                     /translation="MMDLQSWREDIDGELNPDNMRAKLAVGGYAVAMYTYPPGTRFGN
                     HTHAGDKKDGVLSGQFLMEMLGERVTLKAGDILHVPAGVVHCASVLGEEPVVSLDATK
                     L"
     gene            complement(31945..32316)
                     /locus_tag="AC16PP_13055"
     CDS             complement(31945..32316)
                     /locus_tag="AC16PP_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HIR6482602.1"
                     /translation="MSALYALLADLLVVLHLLFILFVVLGGFLTIHWPRLAWAHLPAV
                     AWAVVVEWAGWLCPLTPWEARLRSLAGEAGYQGDFVAHYLLPLLYPQAMTRTTQVLLG
                     ALALAVNAAIYGGWWYLRRRR"
     gene            complement(32325..32696)
                     /locus_tag="AC16PP_13060"
     CDS             complement(32325..32696)
                     /locus_tag="AC16PP_13060"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6482603.1"
                     /translation="MIDGGCFCGEVRYRIEDGDYPAAHCHCTMCRRTSGAPFVSWLVV
                     PKEKFSFTAGTPKQLQSSDRGRRFFCSSCGTPMTCIVDDHPDNVDVTLGSLDEPERFV
                     PRFEIHTDTRLGWVQTHLKQA"
     gene            complement(32706..32966)
                     /locus_tag="AC16PP_13065"
     CDS             complement(32706..32966)
                     /locus_tag="AC16PP_13065"
                     /inference="COORDINATES: protein motif:HMM:NF025715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HIR6482604.1"
                     /translation="MSDEDIRRALQALHTELENADPIDPELVSLLKEVDSDIHRVLEA
                     STPALEGLGDRLEWLAAKLAVSHPRVEGFLRELVDALGKLGI"
     gene            33204..33665
                     /locus_tag="AC16PP_13070"
     CDS             33204..33665
                     /locus_tag="AC16PP_13070"
                     /inference="COORDINATES: protein motif:HMM:NF021525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HIR6482605.1"
                     /translation="MLRFGGVCWLLAIALLLGACDPQDRRPGLWLTGEVADVAPGDWA
                     FSDEYAEIALEVATPYLIPHSVTIWCAQVDGTLYVAAAAPEEKNWPGWVSDDPDVRLK
                     IGDTVYAATLAELEDEAEIAAVRSAYSGKYEVANLGSGASLRYWRVGPRGA"
     gene            complement(33688..34134)
                     /locus_tag="AC16PP_13075"
     CDS             complement(33688..34134)
                     /locus_tag="AC16PP_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482606.1"
                     /translation="MSFLPAGFHVPELLVTPTFSARPLTVDDVVRDYDAVMSSRERLV
                     GTFGSESSWPAADLSLKQNLIDLGWHQKEFQKRRSFAYTLVDPDDTLTIGCFYVYPEA
                     PDGFDASVYLWVRTSHSDMDEAVFSAVKQWLEEAWPFDRLACPGRN"
     gene            complement(34139..34462)
                     /locus_tag="AC16PP_13080"
     CDS             complement(34139..34462)
                     /locus_tag="AC16PP_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482607.1"
                     /translation="MRAVAIIIAAALLAVSPVVTGDRSAAGIRVSESQRYCQAARQCT
                     KVQTRCGGCDCGTAINESFAAEHRDRLAALCGSHEQVECERSCPETRPICVIGVCALE
                     PLRGS"
     gene            complement(34470..34673)
                     /locus_tag="AC16PP_13085"
     CDS             complement(34470..34673)
                     /locus_tag="AC16PP_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482608.1"
                     /translation="MNEIHLLLGLVLSAIGIGYLVYGRRQQAPVFFIAGLGLVSIPFF
                     VGSTSTLMVAGAVVGAAPFLIRL"
BASE COUNT         5712 a        11095 c        11795 g         6163 t
ORIGIN      
        1 ggtgttgctg gagaacgtgc ggtttctcgt tggtgagaag accgatgacc caaggctggc
       61 gaaagcgctc ggggccctct gcgacatcta cgtcatggat gcgtttgcca ccgcccatcg
      121 cgcacaggct tccacccacg gcgttgcgaa gttcgccccg gtggcctgcg ccggaccgtt
      181 gctggccggt gagctcgagg cgttggagcg cgccttgagg gcgcccgccc ggcccatgac
      241 ggccatcgtc gggggtgcga aggtttcgac caagctcgaa gtcctggaca ccttggcgac
      301 cattgccgac cgcatcatcg ttggcggcgg catagccaat accttcatcg ctgccgccgg
      361 tcaccccgtg gggcgctcgc tctacgagga ggacctgctg gaagcggcgc gcagcattgc
      421 cgccaaggtg gaggtgccgc tgccggtgga cgtgatggtg ggcgagcgcc tggacgcggg
      481 cgcggtggcg gtgctgcggg ggctcgacga ggtccgcccc gacgagatga tcctggacat
      541 cggtccggag acggcccgac gttacgcggc agagcttggg tctgcgggca ccattctctg
      601 gaacggcccg ctgggcgtct tcgagttcga ccagttcggc gagggcactc gcgtggttgc
      661 ccaggccatc gcgtccagcg gtgcgttctc catagccggc ggcggggata ccctggcagc
      721 catcgacaag tacgacgtgc gcgatggggt gtcctacatc tccaccgggg gaggcgcgtt
      781 cctcgagttc gtggagggca agaccctgcc cgccgtggcc gtcctcgcga gccgggctga
      841 aggttaacgg tcagcggtcg atggaagtcg gtttcgaccc ggacagcctc aggcaggcgc
      901 ttaagcccct cgtccccggc tctcccacgg tgctgacgcc gcaggaggat gcttacctgc
      961 ggcactacgg cattcacttc gtcgaggagt atcccgacct ggcccatcat ttcggggcgg
     1021 tggaaacgga ttcgcacagc gtcgccgccc atctctggtc gccgcgcgat gcccggggca
     1081 ccgccgtgat cgtgcacggc tactacgacc acacgggcct ttacgggcac ctgatccgcc
     1141 acctcatcga ccgcgggctt gccgtgctgt cgttcgacct gcccgggcac gggctatcca
     1201 ccggcgcgcc ggccaccatc gaaacgtttg atcactacgt cgccgcgttc gacgcctgca
     1261 tcgcggcgct cggcgcgcac ctccccaagc catggcacct gctgggccag agcacgggag
     1321 gcgcggtggc catggagtgg ctcctggcca acggcgtcgc tcgggcgacc agcccgtttt
     1381 cgggcatcgt gctcctagca ccgctggtgc ggccgtacct gtggcccgtc ggccggctct
     1441 tctacgaggt cgtgcggcgc ttcatcaccg agcgggagcg aacgttctcc gtcaacgccg
     1501 agaatccgga gttcatcgag ttcctcaagc accacgaccc gctgcaggcg cgcgtgctgc
     1561 ccgttcagtg ggtcaccgcc atggtggctt ggaagaagcg cttcgtcagc cgcgcgccaa
     1621 gcgatctggg gctgctggtg gtgcagggca ccgccgaccg cacggtcgac catcgctaca
     1681 acctgaagat catcgagcag ctcttcgatg ccaaggtggt ctatgtcccc gaggcgcgac
     1741 accacctggt caacgagtcg gtggtcctcc gccaacagat ttttcaggcg gtggatgcac
     1801 agctcgatgc cgccggatga gcgggggatg acatctgcgc acgggcagct gacctccgcg
     1861 ctatcgcctt cgcaggtccg cactgacccc gacagcctgc acgagtgggg gcgggactgg
     1921 acccggactg ccgagcctgc gcctgccgcc gtcgtttttc ccgagactac cgctgacgtg
     1981 gtcgccatcg tccgcctggc caactccgag ggcctgaagc tggtgccgtc gggtgggcgc
     2041 acgggtcttt ccgggggtgc ggtggccgcg gacggagagg tggtggtttc cttcgaccgg
     2101 atgaaccggg tgctcgagtt cagcgccgca gaccgcctgc tcagctgtca ggccggcgtc
     2161 gtcacgcgga ccgtccagga ctttgcccgg gagcgtgggc tgtcttaccc ggtggatttc
     2221 gcgtcctccg ggtcgagcca gatcggcggc aacattgcga ccaacgcggg gggcatcaag
     2281 gtgatccgct acgggctcac ccgggactgg atagccgggc tcagggtcgt gacgggcgcc
     2341 ggcgagctgt tggactgcaa tcgcggattg gtcaagaacg cgaccggata cgatctcaga
     2401 cacctgttcg tgggctccga gggcacgctg ggtttcatcg tggaggcgga tgtccggctg
     2461 gctcccgctc cggctccgca aacggtcatg ctgctcggcg tgaccgagtt cccgaggctg
     2521 atcgacgtgc tgtccgtgct gcagagcacc gtcgtgctct ccgcgttcga gttcttctcc
     2581 gaacttgcgc tgcagaaggt tctctcgcat cggaagctca ggcgacccct ggccgagacg
     2641 gcgcccttct atgcgctggt ggagttcgac acggcggact tagagcccgc gacggcggcc
     2701 tatgaggccg cggtgggcgg cggctgggtg tgtgacggcg tcgtcagcca gtccgagggc
     2761 caggcggcgg cgctgtggtc gctcagggag ggcatctcgg agagcatcgc gcagttcacg
     2821 ccttacaaga acgacctgag cgttcgcgtc tcggtgttgc cggagttcct cgctcaggtc
     2881 gacggcctgg ttgcccagag ctatcccggc ttcgaggtgt gctggtacgg gcacatcggc
     2941 gacggcaacc tccatctcaa catcctcaag cccggcgggc tctcgcttgc ggagttctac
     3001 gctcgctgcc acgccatcag ccccagcctc ttcgagctcg tggcccgcca cggcggcagc
     3061 gtatctgccg agcacggcgt tggcctgctg aaacgcgact tcatcggcta ctcccgcagc
     3121 gcggccgaga tagagatcat gcgcgcgctc aagggcgtgc tcgatccgaa tcagatcatg
     3181 aacccgggca agctcctctc ctgaggctca tgcgccgcag ggcgcggatc ccggctcctc
     3241 agaacgacgt cacgccggat gcgccggcga cgatgaccag gtcggcggcc tgggcggcaa
     3301 acacgccgtt gcataccacg ccgacgatgt tgttcacggc cgactccatg gcggccgggt
     3361 cggtgatggt cagctcgtac acgtcgagga tgatgttgcc gttgtcggtg acgaaaccct
     3421 cgcgcagctc gggagcgccg ccgaggccga cgatggcgcg cgcggccaga cctctcgcca
     3481 tggggatgac ctccacgggc agagggaacg cgcccagtgt ctgaacgacc tttgattcgt
     3541 cgacgatgca aacgaactcg tcggcggacg ccgccacgat cttttccctg gtgagcgccc
     3601 cgccgccgcc cttgatgagc tgccttgccg cgttcacctc gtcggcgccg tccacgtaaa
     3661 ccgccacggc cgccgcagcg ttcagatcga tgaccggaat gccgacagcc ttgagccggg
     3721 ccgcgctcgc ttcgctgctg gcaacggcgg catcgaattt gtgcctgatg gcgccgagcg
     3781 cgtcgatgaa gtggttggtg gtggagccgg tacccacgcc gacgatgctc gcgctgccga
     3841 gcttgggctc gatgtaggcc agcgctgcct ctgcggcact gcgcttgagc tgatcttggt
     3901 ccatacttac gtccccgaag ccgtgctcga tgcgcggcgc ccacaaaccg ctgcgaccct
     3961 aactcatacg attgccgtgg aaaagtacgt taaaaagatt ctctcctcgc gtgtgtacga
     4021 agcggccgta gagaccccgc tgcagcccgc gcgcaatctg tcgatccgcc tgggtaacca
     4081 cgtgcggctc aagcgggaag acatgcagcc cgtcttctcg ttcaagattc gcggtgccta
     4141 caacaggatc gttcagctca ccgaggagga gcgggcccgg ggggtcatcg cggcgtctgc
     4201 cggcaatcac gcccagggcg tcgcctactc tgcccagcag ctcggaatca aggcgctcat
     4261 agtgatgggg cgcaacacgc cgagcatcaa ggtgaacgcg gtgcgcgccc tgggcgccaa
     4321 ggtcgtgctg cacggggacg cctacgacga ggcggccgcc cacgcgttga agctggccga
     4381 gcgcaacggc tacgtgttcg tccatcccta cgacgacccc gacgtcatcg cgggtcaggg
     4441 caccgtgggc gtggagattc tcaaccagca cgtcgccgtg ccggacgcca tcttcgttcc
     4501 ggtgggcggc ggcgggctga tcgccggtat cgccgcctac gtcaagtacc tcgcgcccga
     4561 tacgcgcatc atcggtgttg aggcggaagg ctcggcgtgc ctggcggcgg ccatggcggc
     4621 cggtcggcgg gtgaggctgc ccttcgagag ccttgatctt ttcgccgacg gcgtgtctgt
     4681 ggctcagatc ggcaaggaaa ccttcaaggt ggccaggcgg tgcgtcgatg aagtcatcac
     4741 cgccaacaca gacgagatct gcgccgcgat aaaagacctc ttcgacgata cccgctcaat
     4801 cgcggagccg gcgggcgccc tggcggtggc cgggctgaag aagttcgtcg agcgggaggg
     4861 cgttcggaac ctgaacctgg ttgccgtggt cagcggtgcc aacgtgaact tcgaccggct
     4921 gcggcacatc tccgagcggg cggaggtggg ggagcaccgc gaagtcgtcc tgggcgtcac
     4981 gatcccggag aagccgggca gcttccttaa gttctgcaag atgctcggca accgccctgt
     5041 cagcgagttc aactaccgat tcgccacgga ccgggaagca caggtctacg tcggcctgca
     5101 ggtatcggcg gccgacgata gggccgagct catgaaaagc ctctccacag gctacgtcgt
     5161 gcacgacatg acggacaacg aggctgcaaa gctgcacgtc cgacacatgg tgggcgggcg
     5221 caccttcggc agcaagccgg agctcctcta tcgattcgag tttcccgagc ggccgggtgc
     5281 gctgcttcgc tttctgaccg gcctggggtc tcgctggaac atcacgatgt ttcattatcg
     5341 taatcatggg gccgcatggg ggcgcgttct ggtgggcttc gaggctcagc cacgcgaccg
     5401 gcgctccctg ggcgagtacc tcaagcgcat cggctaccgc tactgggaag agacggacaa
     5461 cccggcctac cagctgtttc tgcgctaggg gccgttactc ggccagatag cgctcgaact
     5521 cctcgtcgaa tgccagcagg gctggatcct tgagatggtc cacgtacaac cgaccgtcca
     5581 gatggtcgaa ctcgtgctga aacaccgtgg cgaggaaccc tttcagctcc agatcgagcg
     5641 gtttgccctc ctggtcgagc gcgttcacgc gaacatgctg gggccgttcc acgaatcctc
     5701 gcagccccgg aaccgacagg cacccctccc agtagcccgc ggtggcagga tccacgacct
     5761 ccacggacgg gttgatgaac accgtcagcg gcagagcggg gatctcgccg tagcgggtgg
     5821 ggccgccggg aatctcgatg atggccagcc gcacggactc gttgatctgc ggggccgcga
     5881 gacctatgcc gccgtagtcg tgaagggtgt cgatcatgtc gccgatcaga cggcccaccg
     5941 cctcgctgcg gatctcttcg ccggccagcg gcctggcgac ccttcggagg gtggggtgtc
     6001 ccatgcggat gattttgcga acggccatgg gcggagacga tacggggtca ggtgaacgct
     6061 tgccagccgc gctcggtcac gatgccgtcc agagggatgt cccagtcctg gaacggcagc
     6121 gggtcggcgc ttcgctgcac ttcgtgggcg atgccgacga tccggggccg aagcggttcg
     6181 ggcagcgcgc ccaggaaccg gtcgtagtag cccgcaccca tgcccaggcg aacaccgtac
     6241 tcgtcgaagg cgaccagcgg gagaaggatc aggtccaggc ttctgccgtc cacgaaagcc
     6301 gagccggggc ccggctcggg aatcccgtag cggttcacca gcatgggcgc ggccctatgg
     6361 taacggtaaa aggccattct ccctgcggtg ctgactaccg gcagagcgag ctggcggttt
     6421 cggtactcca gctcgccgag caggggcgcg aggctcactt ctccatcgtt ggcccagtac
     6481 gcgccgattc gcctgctgcg ccatgacacg gggttgttga gcaggtgtcg ggctacccgg
     6541 agcgcgtgct ctcgctgctg atgagagccg agggcccgcc tgcgctcacg gaagaaacgg
     6601 cgaagttcct gacgttcgag ggtgctggtc atggcaggga accgggttgg aaagagtctc
     6661 ccgaaaatgc cgctgtttgg tttgccctga accgttgtgg ttcaaggcgg ggacccgggc
     6721 gacgtctaag gcttcccgat ctgcaccacc gtttggataa ccgcgggcgg tatcggacag
     6781 gctcaccggc gtcagcgccg aggtctccta tagtttatct aacggctcaa ggacgacacc
     6841 aactggcgaa cattccagga gacagcacgg agtatagccc aattgacgtc gttgccacgg
     6901 gttgacaacc ggtcacgatc gagaactgtg aaaccgttaa aggttcagct gtttctgttc
     6961 cgagagcgcg cggccgaccc gctcggtgag ctcggtcacc ttctggctga cgacgccgtg
     7021 agtggattcc aaagacttgg agctgttgat gaactcgttg gcgatgttca gcgcggccat
     7081 cacggcgatc cggtccatcc cgaaaatctt gccggactcg cgtatggaac gcatctgctc
     7141 gtcaaggtag cgggcagacc tcgagagcgc gtccacctct tcggcgggac agctgacctg
     7201 atactccttg tcgaggatct tcacgcttac ggtggtggct tcttcgctca tgtcccctgc
     7261 ttcgctaatc ctgctcgagc gccttcagtc gggtgatcat cgattcgacc cgggctttgg
     7321 ccagctcgtt tttctcaatg agcttcgccc gctccgtggt ccagttctgc tgttgtgctc
     7381 gcagcgcccg attctccttg gtcagcacgc tgcatagctc tatgagctca tccactttgc
     7441 tctcgagcgc cgtccattct gtgtcggcca tgccaggctt cacgcgtatt ctgcttatcg
     7501 caggatagtg tcgcctgaat gggctggcat gtgaagcatg taacttctgc tagcattagg
     7561 cctatcccgg ggcacgaagc tcgataggcg acatggcgga cctgacggcg ctcgcacttg
     7621 gtgcggtgga atctctttcg gttgccgaga tccacggcgc cgtgtgcggg ctggcagtcg
     7681 cctccgaggg gggaatcgag ctgggctctc tcgtcgagct ggtgggcatc gaggcccttc
     7741 gggacgaaca ctccgttgga gaatttgtta acgccgcggt ggacgcgctc cacgccgagg
     7801 atctcagctt tgctccgctc ctcccggacg accatgtgga cctggcgctg cgcctggaag
     7861 cgctgggcga atggtgcggc gcgttcctgg ccggctttgc ttcaggatcc cgatttcagg
     7921 aactcggaga agagcagatt ccggaggagg cccgggaaat catcggcgac ttcacctata
     7981 tcgccaacgt tgacggctcg gaggccggcg acggggagga tccggaatcc gcggagaagg
     8041 actatgccga ggtgcacgag tccgtgaagg tcggcgcgtt gcttctgcgc accctcctgg
     8101 gtggcgacgc gcctgaagag acggcgcggc cgtgattgcc gctgcggagt accggcgccg
     8161 ccgtcagcgc ctcatgagcc tgatggacga agggtccatc gccgttctgc cggcggccgg
     8221 ccagcgtttc cgtaaccgca acacggagta tccgttccgc caggacagtg acttctacta
     8281 tctgacgggc tttgcggagc ccgacgccgt tctggtgctg gcgcccgggc gtgcgcacgg
     8341 tcaggtcatc ctcttctgca gagagcgcga ggcgcgcgcc gagcgctacg acggcgagcg
     8401 ggtagggccc gagcgcgccg cggagcgcat tggcgtggac gacgctttcc ccgtcaacga
     8461 cctggaggag attctgcccg gcatgctcga gggacgggag cggatctaca tcaccctggg
     8521 cgaatacccg gagttcgaca accgcctgat gcactgggtc gccggcatcc gcgcgcggga
     8581 agcgggcggg gccattccgc cgggtgagtt cgtcgcgctc aagcatctgc tgcacgagca
     8641 gcgtctctac aaatcggccg ccgagctcag ggcgatgcgg gaggcggccc gtatcacctg
     8701 ctccgcccat cgccgcgcca tggcggcctg tcggcccgga atgaccgaga cgcagctgga
     8761 agccgagctg ctgcacgagt tcatggtcaa cggtgctcgc agcccggcct atccgagcat
     8821 cgtgggggcc ggcgcgaacg cctgcgttct gcactacgtg gacaaccacg acgtgctgaa
     8881 gaagggcgat ctcgttctca tcgacgccgg ctgcgagtat cagcactacg cctcggacgt
     8941 aacccgcacg tttcccgttg ccggtaagtt ttctgctgcc cagcgggctc tctacgaggt
     9001 cgtcctcgaa gccaatcgtc agggcatcga ggcctgtcgc cccggtgcga cgttcatcga
     9061 gcctcactct acggcgctca aggtcatggt ggaggggctc atcgagctga atctcataca
     9121 aggcgatgtg gaggagattc tccagagcga acgctatcgt gaattctgtc cccacaactc
     9181 gtctcactgg ctgggcagcg acgtccacga cgtgggcgac taccgggtcg aaggtgcctg
     9241 gcggccgctg gagccgggca tggtgctcac cgtggagccc ggcatctaca tcccggccga
     9301 cgactctacc cgacacttgg cgccgcggtg gcgcggcgtg ggcattcgtg tggaagacga
     9361 cgtggccatc accaggcagg gccatgaagt gctttcggcg tcggccccca agtcggtgga
     9421 cgacatcgag cggctgatgc gtgggggcgc cgatggctga cgtggtcgtg gtgggcggtg
     9481 ggctgatcgg agcgctggcc gccctcggcg tcgccgatgg gggacgacgg gtcacggtgc
     9541 tcgaacggcg cgagccggaa aggcagcggg gcaggctggg cgtcgacatc cgcaacgtgg
     9601 ccgtatcgcc agcgtcccgc gagcttctgg aagccgtcgg tgtgtggtca tcgctcgagc
     9661 cggctgctta ccaacgcatg gaggtatggg aagagcgagg cactcgcggc atgcacttcg
     9721 acgcggcaga ggtaggccgg gccgagctcg gctggatcct ggaatcgagc caggtcgtgc
     9781 gctcgctctg ggatctgctg cagcggcacc ggaacgtccg gctggaggtc ggcgcggatc
     9841 tggcgagctt gcgcctggcg gcttcgggcg ccgtcttgga aaccgaccgc ggagatatcc
     9901 gggcgcgcct ggtggtggcc gctgacggtg cacgttccag ggtgcgggag ctgctcgcgg
     9961 tgcccgccga agaagctccc accggtcatc atgccctcgc cacggtggtg cgcaccgagc
    10021 aagagcatga gggcacctgc ctgcagcgat tcctgctcga gggcccgctt gcgctgctgc
    10081 cgacggcgga cgcccacctt tcatccgtgg tctggtcgca gcccgcggag gaggccgagc
    10141 ggcgtgcgaa agagcccgac ggcgacttct gcctcgagct ggagcggtgt ctggaagcac
    10201 gatttggaaa ggtggaagcg gtggatgccc gggtcgtgtt tcccctggcg cagacgctgg
    10261 tgtcgacctt caatccccac cacagggtgc tgctgattgg cgacgccgcc agggtcgtgc
    10321 accccctcgc gggcatgggc gcgaacatcg gcttcgagga cgtgcgcgat ctgctggccg
    10381 aactcgccgg gataggcggc gatcaggacc cgggagcgcc gggaatctgg cggcgctttg
    10441 cgcggcagcg acggctacga tcccagatgg tgctggcgct gatgtccggc ttccggcgcg
    10501 cctattctgc ggacggcccg ttggccagct gggttcggaa cacgggcgtt ggatggctcg
    10561 accgtgcaca gcctttgaaa gcacagatca tgagagaggc tttgggggtc gggccgctcg
    10621 ccagaaggtg gtgacgccca ccgtttccgg ggccacccgg ttcgcgtcat cccctgcgtc
    10681 atcgcataca atgtggtgcg tttttccaat ggtggtctga gcatgacgcg gcgaaccccg
    10741 ctatatgagc tgcatgtcgg gctgggcgcg aagatggtcg acttcgccgg ctgggaaatg
    10801 ccgctccatt acggatccca gctggatgag catcacgccg tgcgccggca cgttggcctg
    10861 ttcgacgtct cccacatgac catcgtggat gtcagcggtg cccgcagtct cgacttcctc
    10921 cagcggctgg tggccaacga cgttgccagg ctggaagcgc ccggtcaggc gctgtacggg
    10981 gtgctgctca atccggcagg cggggtgatc gacgacctca tcgtctatcg ccgcgatgcc
    11041 gacttccggc tagtggtcaa tgccgccact cgagaaaagg tgctcaactg gctcgcgggc
    11101 agcaacgagg aagcggtcaa cattctggag cgggacctgg ccatgctggc catccagggc
    11161 cccgcggccg tggagatgta tcagtccctg cctgacagcc tcgaggtccg gggcttgaag
    11221 gcgtttaccg cggccgtgcg cggggaggag atgattgccc gaacggggta cacgggggag
    11281 gacggtctgg agatcatggc cccgggcgat cgcgcccgtc agctttggct tgagctcatc
    11341 gggtccgggg ctcagccagc ggggcttggg gctcgcgaca ctctgcgcct ggaggctggc
    11401 ctgaacctct acggccagga catggacgat gaaacctcgc ccctggtgtc caatctggcc
    11461 tggaccatcg cctgggagcc atccggacgg aacttcatcg gccgccaggc cctcgaggac
    11521 gaacgcgcac gcgggatcgc ggagaggctc accggtatcg tgctcgagga caagggggtg
    11581 attcggcggg ggtgccgcgt agtgaccgcg gccggtgatg ggcacgtcac cagtggtata
    11641 ttctccccga cgttgggata cagtattggg ctcgcgcggg tgccgaaagg cgcgaagggg
    11701 ccgtgcgagg tagaaatgcg gggaaggctg aaacaggcgc gaatcgtccg gcctcccttt
    11761 gtgcgtaatg gtttgcaggt attcgaatga gcgacgttcc acaggagctt aaatatgcgc
    11821 gcacccacga gtgggcgcgg ctggaggagg acggcaccat caccgtcggg atttccgacc
    11881 acgcccagga tgcgcttggc gatgtggtct acgtggagca tccggagccg ggtcagccgg
    11941 ttgcggccgg cgacgaggcg ggcgtcgtcg agtcggtgaa ggcggcctcc gacatctacg
    12001 cgccggtaag cgggagcgtc attgccgtga acgaggcgct ggacgacgcc ccggagaccg
    12061 tgaaccagga tccctacggt gacggctggt tcttcaagat acagccggac gacgttgccg
    12121 agctggacga gctgctcgat tcggaagcct acgccgaact atgcgagaac gaagagcact
    12181 gagtccttga tacccatcta aacggagccc ccgatgccct tcataccgca caccgcggaa
    12241 gaagtgcagg tcatgctgtc ccagatcggc gtggcccgca cggaagacct gttcgacgag
    12301 attcctcaga atttgagagc aggcgcgctg agctcggtcc ccgcgggggc cagcgagctg
    12361 gagatgctgc gcaagctcgg cgcgcgcgcg gcgctcgacg agtcggtagt ctgcttcgcc
    12421 ggggcgggca gttacgacca tcacattccc gcggcggtct gggatctgac gagccgcggc
    12481 gagttcatga cggcgtacac gccctatcag gcggaagcga gccagggcac gctgcagctg
    12541 atctacgagt accagtcgat gatgaccgcg ctcacgggtc tggacgtctc caacgcgtcg
    12601 gtttacgacg gcgcgtcggg cctggcggag gccgtgctca tggcggtgcg aagcaacaag
    12661 aaggcccggt cgcgaaccgt tctgctgccg gactccctgc atccgcacta cgcggccgcg
    12721 gcccgcaaca tctgccggca tcagagcgtg gagttcgtcg gcatggccct ggaccacgac
    12781 ggccgcgtgg atctcgaggc cctggagacc gatggtccgg acgaggtctg cgcggtggtg
    12841 gtgcaacagc cgaacttctt cgggatgctc gaggacgtcg acgccttaac ggattgggcg
    12901 gccgagcggg ggggcctcgt gatcgcggtg gtcaacccgc tggctctggc tgtgctgaag
    12961 cctcccggtg agtggggggg cagcggggcc gacatcgcct gcggcgaagg ccagccgctc
    13021 ggcgtgccca tgtcttcggg ggggccggcg ttcggcttca tctgttgccg ctcggccctg
    13081 gtgcgtcagc ttccgggaag ggtgatcggt cggacggagg acctgacggg gaagccgggc
    13141 ttcactttga ccctgcaggc cagggagcag cacatccgcc gaggcaaggc cacgtctaac
    13201 atctgcacga accagggctt gctggtcacg gcggccacca ttcacatgag cctcttgggg
    13261 ccgctcgggc tcgccaggat tgccgagcat tgccacgcca atacccgcgc tctggtggag
    13321 gccctgaccg ccatcgacgg ggtggaggcg cggcatcagg ggccgttctt tcacgagcgg
    13381 gtgttgcagc tgcccaggcc ggccgacgag gtcgtagccg gcctgctcga gcgcgggata
    13441 ctcggcggcg tggcgctcgg cgcgtatcac gagagcatga atcacgacct gctcgtctgc
    13501 gccacagaaa agcggacgga cgacgagatc ggccgctatg cggccgccct gagggaggtg
    13561 atcgcccgat gagcgatgtg cgaacgatat tcgagctcag cgcgcagaac cggggtgccg
    13621 ccgctcagca cttgagcgag cggccgggag ccatggacga cattcccgcg agcctgcgtc
    13681 gtgagcgggc tcccggcctg cccgaagtat cggagcttca ggtggtgcgg cactacacca
    13741 acctgtcgcg caagaatttc tccatcgata ccaatttcta tccgctcggc tcatgcacca
    13801 tgaagtacaa cccgcggggc gcgcatcgcg ctgccagcct gccgggtttc ctgaaccggc
    13861 acccgcttgc cccggactcg gccagtcagg ggttcatggc ctgcctgcac gagcttcaag
    13921 agatcctcaa ggacgtcacg ggcatgcagg gagtgtccct cacccccatg gccggggcac
    13981 aaggcgaatt cgccggcgtg gccatgatcc gcgcctatca cgaggcccgg ggcgatcatg
    14041 caagaaacga aatcctggtg cccagcgccg cccacggcac caacccggcg acggcggcga
    14101 tgtgcggcta ccgggtagcg gaagtgcccg tcaacgcctc gggcgacgtc gacctcgaag
    14161 ccctcaagct tgcggtagga ccccagacgg cggggctgat gatgaccaac ccgtccacct
    14221 gcggggtctt tgagcgtggc atcgagagca tcgcggcggt ggtgcacgat gccggcggcc
    14281 tgctctacta cgacggagcc aacctgaacg ccattctcgg caaggtgaag ccggggtcca
    14341 tgggcttcga cgtgcttcac atgaacctgc acaagacgtt cgcgaccccc cacggcggcg
    14401 gcgggccggg cgcgggcccg gtgggcgtcg gcgaacggct gctgccctac ctgccggtgc
    14461 cgttcgtcgc ccaggatgca aaaggcgcat accggtgggt aaccgacgcc gagctgcccg
    14521 acagcatcgg ccgtctgtcg gcctacgccg gcaatgctgg aattctgctg cgcgccctcg
    14581 cttacgcgct gatgctcggg cgggaaggca tgcaccgggt gggcgagtac tccacgctca
    14641 atgccaacta cctggcgcga cgtcttgaag atgcaggctt cgagctggcc tatccggacc
    14701 ggcgggcgac ccatgagttc atcttgacct tttcggagtc ggcacgcagc cgggacgtga
    14761 cggccatgga tttcgccaag cgactcctcg attacggcat tcactctccg accacctatt
    14821 tcccgctgct gatacccgag tgctttctga tcgaacccac cgagacggag accaaggcgg
    14881 agctggacgc gttcgtggat gccctggtgg cgattcagcg cgaagccgaa gccgatcccg
    14941 aaaaagtcaa aggcgcgcct tacgatctgc ccgtcaggcg gttggatgac gtcaaggccg
    15001 ctcgcgaact ggacctggcc tggcgcggct agacacgggg ccgggatctc aacgcttcgc
    15061 cgagatcctc cggcttgccg atgggcagcg agcaggacag cccgctgcac acgtatgcca
    15121 ccgtctggct gtgcggatcg tcgctcacgg gtccgggaag gtaggcgggc agcgtgctga
    15181 cgccctcaaa gggtatgcag tagacggccc gccatggcgt gtatcccgac accgcggccc
    15241 ggcgccagtc gggaaacgtc gcggcggcac cgcggacgac gacgagctcc gggggcatga
    15301 tctcgtcttg caggcccgag agcagacagc agtggccggc cggatactgc tccatggccg
    15361 cgcgcgccca cgccagagtg gccgccgcag cctcgaggta gcgcgtctct ccgaaaaggt
    15421 ggcccagcgc cgtgagcgcc tgaaccatca cgccgttgcc cggcggcagc gcgtcgtcca
    15481 tggtgggctt tggccgatga atcagggtct cgtgctggtg ggcggtaaag aaaaagccgc
    15541 ccctgtcggc atcgtagaac cgctccaggg caccgtcagc cagcttgcag gcgaaggcgg
    15601 cgtcctcttc gcgccaccgc gccccaagaa gggcaatcag gccgtcgagc aggttggcgt
    15661 agtcgtccag gtaggccgga tagcggggcg ccccctgcca ggtcgcgtag agcatgccgt
    15721 cccgccagag ccgggcgcgc gcgaaatcgg ccgcctgagc ggccgcatcg agccatcgcg
    15781 actgccccag gcgtgacccg gccctggcca ggcccttgat ggccaggccg ttccaggcgg
    15841 tgagcacctt gtcgtcgagg ccggggcgaa cccgcagctc ccgggctgcc agcatcttgc
    15901 ggcgcgcgct ggccagcagg tcgttcgcag cggcgcgatc catggacagt cgctcgacca
    15961 ccgagcgcca ggcatcgtat cggtgcagat tccacttgcc ctcgaagttg gccggtttgt
    16021 ccaggccgta gagcgtttcg acgaccagat actcgtcctc gtcgagcagc cgtttcacct
    16081 ggtcgcgccg ccacaggtag aacttcccct cctcgccctc ggagtccgcg tccagcgcgg
    16141 cgtagaagcc gccctggggg tgacgcatct cgcgcaggag ccagtcggcg gtatcgctca
    16201 ccgcctggga gaagagctcg tcgggaccaa tggcaagcgc gtcgctgtag agggccagga
    16261 gttggccatt gtcgtaaagc atcttctcga agtgaggaat catccagcga cggtccgtgg
    16321 cgtagcggca gaagccgccg cccaggtggt cgtatatccc gcctcgcgcc atgcgcgtca
    16381 gggtggtcat gacgaactct agcccctgcc ggtccgcctc gcccgacctc tgggcgtagg
    16441 cccagtggcg cagcaggtag gtcacggtct ccggcatggg aaatttgggt gccccgccga
    16501 acccgccctc atcctcgtcg gcctgcgcgc cgagctgctc tctggcagcc gccagcagct
    16561 cggcgtcacc gagctcgccg gcctgtgaat ctccgcctgc cagccggccg agcacgtcgg
    16621 cgacccgcgt gccgtgatct ctcagctcgt cgcgctggct ggcaaacgtc tcggcgatcc
    16681 tcagcagaag atcgacgaat ccgggcagct gatagcgggg cgttttcggg aaataggtgc
    16741 cgccgaagaa tggcagcctc gtgtcggggt ccagaaacat ggtcagcggc cagccgccac
    16801 cctgttgggt gaggagctga tgggcgagct ggtagacctt gtccagatcg ggcctctcct
    16861 cccggtccac cttgatgttg acgaagtgct cgttcatgac ccgggcagtg gccgggtcct
    16921 cgaaggattc gtgggccatc acgtggcacc agtgacaggc cgagtagccg atggagagca
    16981 ggatgggttt ctgctgctcg ctggccaacg cgagcgcggc ttcatcccag gggtgccaat
    17041 ctaccgggtt atcggcgtgt tgccgcaggt aggggcttgt ctcgtcggcc aggcggttgc
    17101 tcaaggttcg aggctctttt cggctgtgca gggtcagcat cctgacacgg aacacgaccg
    17161 ggtcaactcg gtcggcccgt cggggccgca gttggtaatt cggcccatat tgaggttcaa
    17221 tcgagggaaa ttcagaggaa tgccgatggt tgatgcggat ggtttccgcc caaacgtcgg
    17281 aatcgttctg tcgaacagca ttggccaggt gctttgggcc cgccgggttg gcggccagga
    17341 cgcctggcag tttccgcaag gcggcatccg tgagaacgag tcagtacaag ccgcgctttt
    17401 tcgcgagctg ttcgaggaag tgggcctcgt ggcggacgac gtcgaggtgc tggcaagcac
    17461 ccgcggctgg ctccgctacc ggctgccgcg caggatgcgt cgttacaact ccagccccgg
    17521 tttccagggg cagaagcaga agtggtttct gcttcgaatg ctgggcgacg acgcgcgcgt
    17581 gcagatggac gggagcgcca agcccgagtt cgattcctgg cgttgggtga gctactggta
    17641 cccgttggat caggtcgtca gcttcaagcg tgacgtctac cgcgccgcgc tcaaggagct
    17701 tgcgccgcat ctgcccgttg aggatgtggc cagcccatgc tgaccacgct gcggcggatc
    17761 gtccatggag tcgaggaatc cagctcgttc aaggaggcgc tgagcctgct ggtgggcgaa
    17821 gttcgagagg cgctcggcac cgaggtctgc tccgcttacc tgctcaaccc cgaaggcgac
    17881 agctacgtgc tggtggccaa cgaggggctc aataccgacg cggtcgacaa gctttccctc
    17941 ggcctgaacc agggattggt gacgctggtc gcggaacggg cggaacccat caacctcagc
    18001 gacgccaccc accatccccg cttttacctg gtgccggaga tcggcgagga ggcgttcaac
    18061 gcgttcctcg gcgtgcccgt cattgccaac cgccgggtgc tcggggtgct ggtggtccag
    18121 gagcgcgatg cgcgccggtt cgacgagagc gaggaggcct ttctggtcac gctcgccgcc
    18181 cagctggcga cggtgatcgc tcaccacgag gccaccggcg agattcagca gctcctggag
    18241 gacagctcgg tcgagcccat cgcccgggag gacgctcttt acaagggcgt ccctggtgct
    18301 ccgggaatca gtctaggcac cgcggtcgtc atgcacccga cggcgagcct ggaagcggtg
    18361 cccgaacgca ttgccgagga tgttcgcacc gagctcatgg tgctcgaccg agccgtcaac
    18421 gcggtgcgcg acgacatccg gaggataagc gagcagttcg ctcagagcct gcctcccgag
    18481 gagcttgcgc tcttcgatgc ctacctccac atgctcgacg acaacgcgct cgccggtgac
    18541 gtgcgcgatc gcatcaatcg tggacaatgg gcacaaggcg ccctcaaaca ggtgattcgc
    18601 gagcacgtcc ggcgcttcga agccatggag gacccttatc tgcgcgagcg cggagccgat
    18661 gtcagggatc tgggtatgcg ggtgctcgcc tatcttcagg acatccgctc gcggaagacc
    18721 tatttccccg acgacaccat cctggtcggc cacgaaatca cccccagcat gctggccaac
    18781 atcccaccgg aaaagctcaa gggcatcgta tccgtgcgag gctcgagcaa ctcccacgtg
    18841 gcgattctgg cgcgggccct gggcatcccg gcggtcatgg gggccatgga tctgccgatc
    18901 ttcagcatgg agaacatttc cgtcgtcgta gacggtttct acggcgaggt tttcaccaac
    18961 ccttcgctgg ccctggccgg ccagtatctc cagctaatcg cggaagagcg cgagttcgcc
    19021 gaggagctcg aggacctgcg ggagctgccc agcgtcacca aggacggctg gccggtgggg
    19081 ctttgggtca acatcgggct caccggtgac atcacccgct ctctggaccg cggcgcggag
    19141 gggatcggcc tgttccgcac cgaggtgccc ttcatggaga aggagcgctt ccccagcgag
    19201 gaggagcagc gtgccattta ccgggaacac atggaggctt tcgatccgcg ccccgtgacc
    19261 atgcgcacgc tggatgtggg tggggacaaa tcgctgccct acttccccat ccacgaggac
    19321 aaccctttcc tcggctggcg cggcatccgg gtcacgctgg accacccgga aatcttcctg
    19381 gtgcaggttc gcgccatgat caaggcgaac gccggcctgg aatgcctgct gcggatcatg
    19441 ctgcccatgg tctcctcgct ctccgaggtc gaggaagcca aggcgctggt ggagagggcc
    19501 tacgtggagg tcatcgaaga ggggatcgtg gtgaagcaac ccctggtggg ggtgatgatc
    19561 gaggtgcccg ccgccgtata ccaggcgcgc cagctggccc gtatggtgga cttcctggca
    19621 gtaggatcga acgatctgac ccagtacatg cttgccgtcg accgcaacaa cccacgggtc
    19681 gcccagcttt acaaggagat gcatccggcg gttctgcaag cgcttcggga ggtggcccgg
    19741 gcggctcacg ccgaagagaa ggtcgtgggc atctgcggag agctggcggg caccccggcc
    19801 ggagcggtgc tgctgatggc gatgggctac gacgcgctgt ccatgaacgc caccaacctg
    19861 ccgaaagtga aatggaccct gcgcaacgtc aaacgctcag atgcccgtcg catgctggtc
    19921 aacgtcctgc acatggatac cgctgaggag attcagcagt tcatgcagga ctggctgatc
    19981 gactcgggcc tgggccgggt cgttcccagc taccatcact gacacgcacc tcacgcacgc
    20041 gcgacctcga agtccgagcg cctgccgggc acgccctggg gaccaaactc ctgctcggcg
    20101 aagcggatgc cgtccgcgcc gatgatgacg gccgtgctcg ctctggttcc gtagtcatca
    20161 ccccggatga atctcggggc tacctgtcgt tcgagctcga tcgtgcgccc ccggctcggc
    20221 agctccgaat ccggtggctg ggtatcgtcc tgcagcaggc caagcaagtc gtcgaccgtc
    20281 gggcccgagc tgagcgttcg ccggagcgca ccgaccccgc tcgtcacctt cggccaggtc
    20341 gccccgagct ccgcgttcgc caggccgtag atgccaggcg ccagatcctg ggtggtgccc
    20401 cggttggagg tgtagaccag ggcgtccccg tcccagacga gcaggttgaa gccgcggtag
    20461 ctgggcccgt cgaggtggtg tgcgtagtcg tgtgccgaga cgtcgctgtc aagaaaggcg
    20521 gtcggcagct cgccgcggga gcggggcgcg tcctcggcta cttcctcggc gaagttggtg
    20581 accgtggcga agcggccgcc tcgtgtgacg ccgagccagg tgccgccggc gctatcgtcc
    20641 cggccggcca gaagatgggg cctgtcggcc caataatgtg cgctgcgcgc gggtcgcgcg
    20701 tatagttcgt cacggttggc cgctacgacc aggggaaagg gcgactcggg ctggtaggcg
    20761 attaagacga gacacatgct ctttcgcgcg gctgctggtc ggaatggaac ggcgtggcaa
    20821 gaaatttcag tgtaacagac ccggcctgcg gaggttgtcc tgcactatcc tgacatcgac
    20881 cccgtcatct tcgccatcgg gcccctggcc atccgctggt acggggtggc ttacctctgc
    20941 gcctttgcca tgtgctggtg gctcggggtc gtgcgggcgc cggcgaagtg gtggactcgg
    21001 gaggaggtgg ccgacatcgt cttttacggc gcggtgggcg ccgtgctggg gggccgcgtc
    21061 gggtacgtgc tgttctacgg gttcgaggag ctgatccggg acccgctctt tttgatccgc
    21121 atctgggacg gcgggatgtc gttccacggc gggctgctgg gcaccctgat cgcgctgtgg
    21181 tggttcggga accggacgca ccggagcttc ctgcaggtca gcgacttcgt ggcgccgctg
    21241 gtgcccgtcg gtctgggatt cggacgtctg ggcaacttcg ccaacaccga gctgcccggt
    21301 cgcgccacgg actccgtctt cggcgtgatc tatccctgct cggcggagtc catccgcagc
    21361 atcaacccgc tgtgcacagg acagtgggag gcctttgcac gccacccttc gccgctttac
    21421 caggcctttg ccgagggcgt cgtgctgttc gctctggtgt ggttggtctc cgggcggtcc
    21481 agcaggacgg gggtggtgtc gggcaccttt ctgatcggct acggggtttt gaggttcgtc
    21541 accgagttct tccgccagcc ggatgcccac ctccagttcg aagcgctgga gtggctgacc
    21601 atgggccagc ggctctcgat accgatggtc atcattggta ttctgcttct gttctggccc
    21661 cgcttgcgtg gagggggcga aacgcggggc tgacaagcga gcgccaacag ccggcactgg
    21721 ccccgatcgc cacagcgggt ccggtaatca cggggccgcg agcgcggccg aactcgggac
    21781 gagcagccaa cggaagccag tgaaacagta tctcgatttg atgcgccaag tgcgcgatac
    21841 gggcgtcttc aaggacgacc gcaccgggac gggaacgtac agcatcttcg gtcaccagat
    21901 gcgcttcgat ctgagcgaga gctttccgct ggtgacgacg aagaaggtgg tgctcaggct
    21961 gatcatccac gagctgctct ggtttcttca gggcagcacc aacatcaagt acctcaccga
    22021 tcacaacgtg cacatctggg acgagtgggc ggacgaagag ggcaacctgg gacccgtcta
    22081 cggatctcaa tggcgttcct ggccgacgcc ggacggaggc accatcgatc agattgccgc
    22141 cgtcgtgcag tctcttcgca ccaaccccaa ttctcggcgc cacatcgtga gtgcctggaa
    22201 cgtcgcggag gtggacagca tggcgctgcc gccctgccac accatgtttc agttctacgt
    22261 ggcccagggc cggctttcct gccagcttta ccagcgcagc gccgacatct tcctgggtgt
    22321 tcccttcaac atcgcctcct atgcgctgct caccatgatg atcgcccagg tctgtgatct
    22381 gcagcccggc gagttcattc ataccttcgg tgacgcgcat ctctacgcca atcatctcga
    22441 gcaggcggac gagcagctct cgcgtgttcc gctgccgctg cccacggtca cgctgaatcc
    22501 ggaagtgcgg gacattttcg gtttccgcta cgaggacttc accctcacga actaccgctg
    22561 tcacggcacc atcaaagcgc cgattgcggt ctgacgcgcg gtgcacgtcg cgctgatctg
    22621 ggccatggcg cgcaaccgcg ttatcggccg tgacaaccgg cttccatggc gcctgccccg
    22681 ggacatgcag tttttcgtgg ccacgacaat gggcaaaccg gtgatcatgg gtcggcggac
    22741 gttcgagtcc atgaaggcgc cgctggccgg gcgcaccaac atcgtcgtca ccagcgatac
    22801 cggttatcgg cgcgagggag tgctggttgc ccacagcctg gaggccgccc tcgacctggc
    22861 gcaggcccag tgccgggcgg atgacgtcga agaggccatg gtcatcggcg gcgcggaggt
    22921 ctatcgagtg gcgctgccga ttgctacccg gctctacgta acgttgatcg atgcggacgt
    22981 ggaaggcgac accttctttc cggagatcga cctggacgca tggcgcgagg tgagccggga
    23041 agcgtttccc gcggacgccg gcaacgggct gccgttttcc atcgtcgtct tcgagcggcc
    23101 ctaggtggcg ggccgggtga cgccgcgtcg tatcaagggc ctttgctcgc cgttgggctg
    23161 gattctgctc gtcctgatag cggggtgcgc gcgcgcccca gcgccggttc cggaacctga
    23221 ggctgggcgg cccggcaacg tggtggtgtc gggcgttccg gccatacccg cgggcgcggc
    23281 acatgggctc atgcgttacc ggaacgtccg atctgcctac ctcatgggct gggccgatga
    23341 cggactggtc atcgccaccc gcttcggcga aaccactcag ctgcatcggc tgaagacgcc
    23401 gctgggcgcg cgggagcaga ttacgttctt ctcggaaccg gtcctggcgg cctttcttcc
    23461 cacgggcgct ggcgctttgg gcgctccggg ctttgtcttg agtcgcgacg cggggggctc
    23521 agagcactat cagctgtttc tcttcgactg gtctacgcgt catgcgcgca tggttagcaa
    23581 cggccgggcc cgctacacca acgtgctctg gtccaaccga ggcgaccagt atgcttacac
    23641 caccacggag cgggacggcc gccgctggga catccacctc caggatcttg ccgggggaat
    23701 aaaggtcgcg ctggaaacct ctgatggagg ctggcgagcg gtagactggt cccccgggga
    23761 cgatcgcctg ctgctcatca agtacgtttc ggcgaccgag tcttatctgt acgagctcga
    23821 actcgcatcg ggcagcttga gaccgctgct ggacgaaagc cttgccgtgg ccattggcgg
    23881 cgccaggtac gcggggaacg gcgaaagcgt ctacttcacg gccgacctgg gcgccgagtt
    23941 catgcgtctg catcttctcg agctcgcgac caacagtgtc gaggtgctga ctgctgacgt
    24001 accctgggac gtcacggaga tcgccgttac ctcggatggg gcccacctgg ccatggtgac
    24061 caacgagggc ggggtctcgc ggctgggggt tttgcggttg cccgaccact cgcccctagc
    24121 gctgccggaa gtgcccgcgg gaaccatcta cgggctgcga ttgtcgcccg acggccggca
    24181 gctgggcttc acggtaaatg cgcccaccgc ccccggcgac gtctacagca ttgacctcgt
    24241 tgggcgaacg cttacccgat ggacccgaag cgaggtgggc ggtttggatc cgtcggcctt
    24301 tgccgcgccg agcctggtgc attaccccac cttcgatcgg gaaggaggcg cgcggcggaa
    24361 gattcccgcc ttcgtctaca tgcccgacgg gccggggccc catccggtcg tcatccacct
    24421 gcatggtggt ccgcacagcc agtaccggcc gaagttttcc gcatcggccc agtactacgt
    24481 aaaggagctt ggcctggcgg tggttgcgcc caatttccgg gggtcgtcgg gttacgggaa
    24541 gaactacctc aagctggacg acggttatct tcgcgaggac gcggtccgcg acgtaggtgc
    24601 tctgctggac tggatcgcgg cccagccgcg tctggatgcg gaccgcgtcg ccgtcagcgg
    24661 cggctcctac gggggctaca tggcactggc ctccctgatt cgatacggtg accggctggt
    24721 cgccggcgtc tcccgggtgg gcatcagcaa cttcgtcacg ttcctggaga acacgcgcgc
    24781 ctaccgtcgc gaccttcggc gggtggagta cggagacgag cgggatccgc agatgcgcgc
    24841 gtttctcgaa gggatctctc cgctcggcca ggctgaccgg atcgacagcc ccctgctcgt
    24901 gatccacgga ttcaacgacc cgcgagtgcc cgcatcggag agcgagcaga tcgccgacgc
    24961 cgtgagcgcc gcgggtacgc cggtctggta tgtcctcgcc atggacgaag ggcacggatt
    25021 ccgcaagaag gccaacagcg attacgcggc cgcggttacg gcccttttcc tgcagcgcta
    25081 cctgctcgat gactgaaggg ccgggctcac gagctggggt ggccatctgc gtcaatccca
    25141 tgtccggtcg agacgtcagg cgtctggccg cgcgcgcaag caacatgact cacgaggcca
    25201 aacgcgacat ggtggcgcgg gtggcagccg gcgcccaggc gatgggcgcc agtgacatct
    25261 acgtgacccg ggagccgttt cgaatcgcgt cgttggcttt ggagcacatg gggctcgccg
    25321 cggccgtaca cgtgcttcag acgccgctga ccaatggacc tgccgacacc gaagcggcgg
    25381 tggcggcttt tcttgccgcc ggctgccggg taatcgtctc gctgggtgga gacgggacga
    25441 accgggccat cgcccgcgcc cttggggatg cgggcgcagc gggtgcgggc gttgacctca
    25501 taccgctttc cacgggaacc aacaacgtct ttcccgttct ggcggagccc actgccgcgg
    25561 gtatggtggc ggggctgata gcctctgggc ggctgcccga cgacgatctc cggcagacgg
    25621 ccaaggtaat tcatttgtcg ggcaccgatg cccggggacg agccgttcgg gaccttgcgc
    25681 tggtggacgc ggtgctgctg cgtgatgacc acgtgggcaa cctcctgccg ttcgccgcgc
    25741 agcggctagc tcgaatcgtg ctgacccggg ccgagccgga tgccgtgggc atgtcgccca
    25801 tcggggggat gctcgatgtg gtcgaggcgg gggacgacga aggcctcctg gtggagatgg
    25861 gtccgggcag ggagttctca gcgccgctct cgcccggcgc tttccaggcg gtttcggttc
    25921 gctcggcctc gcgtctgccc cttgatgtga cggtccccct ggcagggccc ggtgtgatcg
    25981 cgctggacgg tgaccgggac tacaagattg ccccccggga ggcgctcacg gcccgtatcc
    26041 gtcgggatgg ccccagggtc gtcgacatag ccgcggcaat gcgtcgtgcc gtcgctcggg
    26101 gtatcatggc gcccgcccgc aacggttaga cccatgatca tcacgccccg cattcgtgga
    26161 ttcatctgca ccacagcgca cccggccggc tgcgccgcgc acgtcaaaga gcagatcgat
    26221 tacgtctccg ggcaggcagc cctggtcggt gggcccgtcg tgcgcaatgc cctggtcatc
    26281 ggcgcctctg gcggatatgg cctggcctcg cgaatcgtcg ccgcgttcgg ctgcggcgcc
    26341 gctacccttg gcgtttctct cgagaaaatg ccgagcgaga cgcgcaccgc cagcgccggc
    26401 tggtacaaca acgtcgcgtt cgaggaggca gcggctgcgg ccgggctcta tgcaaagacc
    26461 ctggacggcg atgcgttttc ggacgagatg aaaggccgcg tcatcgacac cctcaaggcc
    26521 gacatgggcc ccatcgacct gctcatctac agcctcgcat cccccgttcg gcagcatccg
    26581 cgtaccggcg agttgcaccg gtcggtgatc aagccgctgg gccaggcgct gcacatgaag
    26641 tccgttcacg tggaccgtgg cgaggtggtc gaggtggacc tggagcccgc ggacccggag
    26701 gagacggcgg cgacggttgc ggtgatgggc ggcgaggact gggaattctg gatcgatgcg
    26761 ctgaaggacg ccgggcttct ggctccgggg ttccgcacgc tggcattcac ctacatcggt
    26821 acggagctga cctggccgat ctactgggaa ggcaccctgg gcaaggccaa agaagatctg
    26881 gaccgagcgg cgggcgccat ctccgaacga ctggaggatc tcgaggggaa cgccagggtg
    26941 gcggtgctaa aggccgtggt gacccaggcc atgagcgcga ttccggtcgt tccgctctat
    27001 gcgtccctgc tcttcaaggt gatgaaggag cgcggtgtgc acgaagaccc gatacagcac
    27061 attcaccgta tgttcgcgac ccagctctgc acgggcggcg ccctgcgcct cgacgatgcg
    27121 gggcgcatac gcatggatga cctggagatg accgatgcgg tgcaggagga cgtgcgcgac
    27181 cgctgggaac gggtcaccac agagaatctg tccgagcttg gcgatctgga cggctttcgc
    27241 gaggagtttc tgaagatttt cgggttcggc atcgagggcg tggattacga tgccgacctc
    27301 gaccccaccc tgggtcgcgc cccctgatac gcagcgggtc ctcccgccac ctgccaggaa
    27361 actgacagcg cagctggtcg atgtcgttgg cgcggagaat gcggtcgcgc caaagtcgcg
    27421 accatggccc tgcgaaacct tcacctgcgc gcgattcctt tccgctacgc ggggtattcg
    27481 ggtctcgcgc tgaccatttt ggcccttcag ggcacgctga ggccggcctg ggccctcggg
    27541 gtcggcgctg ctggctgcct gatctggacc atcgccgcac atggggcggg ccggctcgcc
    27601 cagaccaggg gcacgtccac tgttccattc gctctgggcc tgcatctcgc agaagcgctg
    27661 ctggcgggcg cgctgatcgg ctggctgtcc ctgccctggc ttcccctgct ggtggcgatc
    27721 accgtgctgc ttgccagcaa cgccgctcag gccggctggt ggcttgcctg gcgagccggc
    27781 atcgccgtgg cggtgggcat aggcgcgggc cgtctcctgg ccgttgcccc ggtcatgagc
    27841 agtacgccag cggccgatct gacagcctcc gtgctgctgc ttggctacac cgtcgggttg
    27901 ggtctcacca gctttgccaa agctcagcgc ctgcacgccg ctcagcagga tctggcaagg
    27961 cgggctctgg tcaccgaacg gctcaacagc aggcttggcc gttatctgcc gccgccggtg
    28021 cgcgcccggg tgcgccgccg gccggacgag cgctgcggcc tcgaacgtcg ctggctgacc
    28081 attgcctacg tggacgtcgt cgggttcacc gagctcgctg gccggcttgc gcccgaggag
    28141 ctggccgtgg tggtcaacga ctacttcgcg gccagcggac ggctgttcgc ctcgtccggg
    28201 ggcaccctgg ctggtctgca gggggacggt ctgctcgcgt acttcggcaa cgcaaccgca
    28261 gccacccggg gtcgcgccgc cgtggattgc gtggagtcct gcttgcaggt gtcgcgcctc
    28321 ctgcgggagc tgagcgagtg ctggcagcgt cgcggctacg tcacgaccct gacgacccgc
    28381 tgtggggtgg caagcggcta ctgcacggtg ggagactggg gcgaggagcg gcttgatttc
    28441 accgtcatcg gcgccccggt gaacctggca agcaggctgc aggcccacgc cggccacgac
    28501 gggattctga tctgcgagcc caccgctgcg ctcgtaggcc aggtcttccc cctgggcgcg
    28561 gagcgcgagc tggtgctgag gggcatcggg aaggtaaagg cgaggaggct cgaaagcgcg
    28621 ctggttgacc ccccgccggc gtctgctaag gtgccgcgtc cggaagtgtg agcaccatga
    28681 ccaagacctt tcttcaggcc tgcaggggcg aagcgacgcc tcatacccca atctggttga
    28741 accgccaggc gggccggtac atgcccgagt accatcaggt caagggtgac atgccgagcc
    28801 tggacttttt caagaatcct gaacgcgccg cccgggcgac gctcgatgct cagcgcatcc
    28861 tcggcgtgga cgccgccatt ctgtttgccg atctgctgcc catcctggag cccatgggat
    28921 tgcgtctgga ctatcagccg ggcgtcggcc ccgtcttcga taacccgatt cgatccaccg
    28981 ccgacgtcga cgggctcgtg agcctgccgg cggaagaggc cacgccctac atcgcggaga
    29041 ccgtgacgaa cattctcgag gagctgccgg gcgaagtcgc cctcatcggc ttcgcgggcg
    29101 cgcctttcac actcgcctct tacgccatcg agggccgtgg ctcgcgcaac tacgtgttcg
    29161 tcaagaagct catgtatgag cacgaggcgg tctggcatcg cctgctcgac aagctggtcg
    29221 agcaggtggc gagttatctg cacctgcaga tccgtgctgg cgtggacgcg gtgcagctgt
    29281 tcgatacctg ggtggggtgt ctttccgtgg cggattttcg ccgctacgtg ctaccccaca
    29341 cccggcggct gatccaggcg atcaaggaag gatcgggtgg ccgggtcccc gtgatctact
    29401 tcggaaccgg caattcgcac ctgcttgccg acgtggttgg agccgggccc gatgtgctcg
    29461 ccctcgactg gcgtgtcccc ctgggggaaa cctggaaagc gctcggctgc acttcgatcc
    29521 agggtaacct cgatcccatc gtgctctgcg cggaccgcga gacggttgcc gagcaggctc
    29581 aggtcgttct cgaccaggcg gcggggcgca acgggcacat cttcaacctc gggcacggga
    29641 tcattccgga aacgcccgtg gacaacgtga agtttctggt ggatttcgtg catgagcgat
    29701 cggcgcgtag cgcctgagcg aattgcggcg gggctgctgc tggccgcctt ctggctgccg
    29761 ctggcggtct gcacctacct ggcgctgcac ccgagcccgc ccgacgccgt gttccgattg
    29821 agcgacgtgc tcctgcacgg ctttgcgttt acctatctga cccttgccct cgcgctggct
    29881 catgggcatc ggcaccgtct tttgcccggg ctctggatgc tgggctacgg cgtgctgatc
    29941 gagttgctgc agagcttcga gacgtcccgt tccgccgagg tcggtgattt gctcgtggac
    30001 gtcgcgggaa tcggcctggg gcttctcgca tacggatggg ccggtgcttt cctgcgcgcg
    30061 ctggcggtgc gtatcgcgag cttggtggtc ggcgcgggcg cgtcgaatgc agactcctga
    30121 cgccagcgct cgttcttgga cgtccgcggg agccggtctg gccagctact cttgcccgag
    30181 cacctcttcg atgagcgcca ggtggctgcc gaggaaaatg tccgaaacgg cccggttggc
    30241 gatgatgcgg tcccggccga tgagataggt cactcggcgt actccgagcc cgagggggcc
    30301 gtcgacgccg tacgcgcgga tgaccttctt gctggtgtcg cacagcagag ggaatggcag
    30361 gccgtaggtt tccgcgaagc gccgatgact cgcctgattc tgagggctga tccccacgat
    30421 cctgacgtcc agattgctca ggtcctcgtg gcggtcacga aacgcgcagg cttcgcgggt
    30481 gcagaccgca gtgaagtcgg ccggatagaa gtagagcacc acgggaccgc tatcgagcag
    30541 cgtggcgagc ttgatgggct cgccggcctg gtcggggagc tcgaaatccg gagccgtatc
    30601 acctttgtcg agcatgggga cccctcgtca gcgtgccagc tcgaaagaga tcggctctcc
    30661 caggaaccct tccaaaggca gctccccgaa gttcacggcc ctgccgtggc tcatggcctc
    30721 ttccagcgtg aggccctcgt gcctcaccag ataggctacc cagaggtggc tcgcccgtcg
    30781 cccagaagca cagtgcagga ggaccttgcc ctcggtgccg gcgatcgcgg cggccagctg
    30841 gtccactgct gagggcgagt aggggctttt aggcccgccc gccgggatgt ggacgtaacg
    30901 catacccagc gattctgcct ttgctttctc gtcgaatggt acctgctgcc ggttggtcat
    30961 ctcggccgcg gtccgcaggt tgacgatggt gtcgacgccg agcgccctca tgcgggtgag
    31021 tgccgaggcg tccggctggc caccgatgta gacctgcgcc gtgcgggcca gcacgccgtt
    31081 gaagtcgccg gcggcctcga cgacggggac agggaactcg gttgcagccg gcgggggttc
    31141 ggcaccgacg ccggggaagc tcatcgcggt gatcagggcg aggagcagga ggtatggcag
    31201 tcgcattacg gacgcgctcc tgaaatttcg tcagttgatg tcgtcagtct tgggcggccg
    31261 ccgctcacgt cgtacacgag ctcggcgccg tcagcgaata ctaccctcag accgacgatc
    31321 gtctgctccc cttcgatggg ctcggccagc gaaatcacgg cgtcgcggcc aaaggcgaag
    31381 ggaaggtccg tagtcaggtc gcgccagcgg cgcggctcgg aaggtgccgg ccccgtcaga
    31441 atcgcgcaac ttcccgttgc atcagggcgg gcgtggatca cgcgcccgtt cttgagctcg
    31501 atctccaaag aacccaacga tgagggtgcg gaggggacgg ggacgtcgcg aatggaggcc
    31561 accttgcccc cgatcagagc cgtgcaggcg ctgcctgacg cctggctcaa agttttgtgg
    31621 cgtccaggct gaccaccggc tcttccccca gaacgcttgc acaatgcacc acgccggccg
    31681 gcacgtgaag gatgtcgccg gctttgagcg tcacacgctc gccgagcatc tccatgagaa
    31741 actgtccgga aagcaccccg tctttcttgt cgccggcgtg tgtgtgattg ccgaaacggg
    31801 ttcccggagg ataggtgtac atggccacgg cgtaaccgcc gacagcgagc ttggcccgca
    31861 tgttgtcggg gttcagctca ccgtcgatgt cctcccgcca gctctgcaga tccatcattg
    31921 tcgctcctgt caggggatta tccgtcaacg ccgccggcgg agataccacc agcccccgta
    31981 gatcgcggcg ttcacggcca gcgcaagggc gcccagcaga acctgggtcg tgcgggtcat
    32041 cgcctgagga taaagaagcg gcaggaggta gtgggcgacg aagtcacctt ggtagccggc
    32101 ttcgccggcc agggaccgca gccgggcttc ccacggagtc agggggcaca gccagcccgc
    32161 ccactcaacc acaacggccc atgcaactgc gggcaggtgt gcccacgcga gccgaggcca
    32221 gtggatggtc aggaagccac cgagaacgac gaacaggatg aacagcagat gcagcacgac
    32281 caacaggtcg gccaacagcg cgtacagcgc agacaagata gcgctcaggc ctgcttcagg
    32341 tgggtctgaa cccagccgag ccgggtgtcg gtatgtatct cgaacctggg tacgaacctc
    32401 tccggctcgt ccaggctgcc gagcgtgacg tccacgttgt ccgggtggtc gtcgacgatg
    32461 caggtcatgg gcgtgccgca gctcgagcag aagaaccgcc gcccgcgatc ggaagattgc
    32521 agctgtttcg gtgtgcccgc cgtgaatgag aacttttcct tgggcaccac gagccagctg
    32581 acgaacggcg cgccgctggt gcgacgacac atggtgcagt ggcagtgagc cgcggggtaa
    32641 tcaccgtcct cgatgcggta gcgaacctcg ccgcagaaac atccgccgtc gatcatcttc
    32701 gcctcctaga ttcccagctt gccaagagcg tccacgagct cgcggaggaa cccctccacc
    32761 cggggatgag agaccgccag cttcgcggcc agccactcca gcctgtcgcc cagcccctcc
    32821 agcgcgggcg tggacgcctc cagcacccga tggatgtccg agtccacttc cttgagcagc
    32881 gacacgagct cgggatcgat ggggtctgcg ttctcgagct cggtgtgaag cgcctgcagc
    32941 gctctgcgga tatcctcgtc gctcatttgc tgtctcctcc cgttgggttc gttcggcggt
    33001 tgccgggttg gccgctgacc ccgttcctgc gggctggccg ctgcggcaac gtcggcccgt
    33061 tgccatctca agagccaatc tgccaataga tcgccctgaa atgcaaacgc gctccgccgg
    33121 cccgtcgcgc ttttcgagtc gtggtgacaa gccgcgcttt cgctcctagt atcgtcgcgt
    33181 tgtctgctgt ttggggagcg aaaatgttgc gcttcggagg agtctgttgg ctgcttgcca
    33241 tcgcgttgct gctgggcgcg tgcgaccctc aggaccgccg gccggggctg tggctcaccg
    33301 gcgaggtggc agacgttgcg cccggtgact gggcattttc ggatgagtat gccgagatag
    33361 cgctggaagt ggcgacaccg tatctgatcc cacattcggt gacgatctgg tgtgcccagg
    33421 tggacggtac cctgtacgtc gccgcagcgg cccccgagga gaagaactgg ccgggctggg
    33481 tatccgatga ccctgacgtc aggctgaaga tcggcgacac cgtctacgcc gcgacccttg
    33541 ccgagctcga ggatgaggcg gagatcgcag ccgttcgcag cgcctattcc ggaaagtacg
    33601 aagtggcgaa cctgggctcg ggcgcgtcgc tgcgttattg gcgggttggc ccgcgaggcg
    33661 cgtgaccggg gcaactgctc gagcgggtca gttgcgcccc ggacaggcca gccgatcaaa
    33721 gggccaggcc tcctccagcc actgcttcac agcagagaaa accgcctcgt ccatgtctga
    33781 gtggctggtg cgcacccaca ggtagacgga ggcatcgaat ccgtccggcg cctcgggata
    33841 aacgtagaag cagccgatgg tcagggtgtc gtccgggtcg acgagcgtgt aggcgaacga
    33901 gcggcgcttc tggaactcct tctggtgcca gccgaggtcg atgagattct gcttgaggga
    33961 aagatcggcg gcgggccaac tcgattccga gccgaacgtg ccgaccagcc gttcacggct
    34021 gctcatcacc gcgtcgtagt cgcgaaccac gtcgtccacg gtcagcggtc tggcgctgaa
    34081 ggtgggcgtg accagaagtt ccggaacgtg aaaacccgcc ggcagaaagc tcacgagact
    34141 atgatcccct cagaggctcc agggcgcaca ccccgataac acagatcggc cgggtttcag
    34201 ggcacgaccg ctcgcattct acctgctcat gactgccgca cagcgcggcc agccgatcgc
    34261 ggtgctcggc ggcaaacgac tcgttgatgg cggtgccgca atcgcagccg ccgcagcggg
    34321 tctggacctt ggtgcattgg cgtgccgcct ggcagtagcg ttgggattcg ctgacgcgaa
    34381 tgccggcagc cgatcggtca ccggtcacca cgggtgagac cgcgagcagc gcagcggcga
    34441 tgatgattgc aacggccctc atggcgggct cacagcctga tcaggaaagg cgccgcaccg
    34501 acgacggccc cggcgaccat aagcgttgac gtggagccga cgaaaaatgg gatcgaaacg
    34561 agccccagac cggcaatgaa aaaaacgggc gcctgctggc ggcgtccgta taccagatag
    34621 cctatgccga tggcgctgag caccaggccc aagagaaggt gaatttcgtt catccgctcc
    34681 tcagcgattg ctggcgggcg gactataaca gcgtgctgaa cacctttgca gacgccccgg
    34741 gatcgatgcc tgaagctaac ggtag
//
LOCUS       DBNHKV010000402         4372 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1119708, whole genome shotgun sequence.
ACCESSION   DBNHKV010000402 DBNHKV010000000
VERSION     DBNHKV010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1119708"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="AC16PP_13090"
     CDS             <1..285
                     /locus_tag="AC16PP_13090"
                     /inference="COORDINATES: protein motif:HMM:NF046101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA3496 family putative envelope integrity
                     protein"
                     /protein_id="HIR6482609.1"
                     /translation="LEEREEFSDQTEFVDEDVSEDDAEIERGDGEFGDDEALGDDLDD
                     YDDDVGAVDDELTLKEQNARSLAIRRAIEQRLERKRLDEDLDYLDFDFDE"
     gene            321..2498
                     /gene="selD"
                     /locus_tag="AC16PP_13095"
     CDS             321..2498
                     /gene="selD"
                     /locus_tag="AC16PP_13095"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR6482610.1"
                     /translation="MQLSAPPVRDLVLVGGGHSHVQVLKSFGMRPVPGTRLTLVSREV
                     YTPYSGMLPGYVAGFYDWHDIHIDLGALASFAGARLIVDEVTGADLDGGRLRLAAHPD
                     LHYDLLSINVGAVPRGVGQGVPVKPIGRFLPQLERVREEAGPGTSIAVVGGGAGGVEL
                     ALAMSRTMPHGVEVKLVTDELLPGYPAGVVRRLEKAVLRSGVVLIRDFRALDCRHGLL
                     ESSDGRSTAADHAFWVTGVMAASWPAAAGLATDVSGFIEVDRCLRSTSHESVYAAGDV
                     ACLIDQPRPKSGVYAVREGPALTRNLRRAIVGRPGIPFRAQKRFLSLIGTADGSAVAA
                     RGPWAASGRWAWRWKDWIDRRFVARFNRLPVMTEPGWSLPESLRDEAPDAMRCGGCGA
                     KLGASPLMRVLERLPPQVSGNVRVGIGDDAAILGATGGETILTVDGFRSLVSDPYLFG
                     RITAHHALNDIIAMGGKGTAALAMASVPLMSEALMEEDLFQLLRGAVDVLNAHEVPLV
                     GGHSSEGAELSLGLTVAGVPDGPTLTKGGAEPGDALVLTKPLGTGSILAGQMRGAVRS
                     DSVSAAIASMDASNASALVVFRRYGIHAATDVSGFGLLGHLGEMLRASALGVRLDLNA
                     VPFIEGAVEAVSAGAVSSLQDNNELALADFALEGCEPGDATVRLLVDPQTSGGLLGCV
                     PEAEAEACVRDLREVGYPRAAVIGRVLSNGWTVTAEREARPLS"
     gene            2565..3716
                     /locus_tag="AC16PP_13100"
     CDS             2565..3716
                     /locus_tag="AC16PP_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016984857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR6482611.1"
                     /translation="MSTEQDTDVIRTIVSQDLLDVLVRVGLIGILLVMSFRIFSPFLS
                     ILLWALVLAVSLYPVHLKLVGKMGGRQGASATVIVIVGMLLIGTPTIALGSLFAGYIH
                     ETYQAFATNSVTVNPPRPEVAEWPIIGERMYAAWSAAASDMPAFLEELQPQLGVLTDR
                     ALSTAASTMGDVLLFLGALIIAGIMMAFGQSGSDAMGRILSRLSGPENGPRLHNLTTA
                     TIRSVATGVIGVAFIQALLLGVGFAFAGIPAAGLLALVVMLVGIVQLPALLVTLPVIA
                     YLWWGGDASTTSNVIWTIYLLVAGMADNVLKPLLLGRGVDAPMPVILIGALGGMVSAG
                     IVGLFVGAVILAVGYQLFMDWVNGGAGAETSPEGSAAELEGGTVPEPRA"
     gene            3721..>4372
                     /locus_tag="AC16PP_13105"
     CDS             3721..>4372
                     /locus_tag="AC16PP_13105"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR6482612.1"
                     /translation="MFSPSKSPTASVLVLLGVLAAQGACTVLGPDYEEPEVGWLGDWQ
                     TDLYGRLEEPEAGTQSDLSFWWMLFGDPTLNALVETAKRDNIGLRVAGLRILESRAVL
                     GIAGSARYPQVQQVTGAVTYVTSREHGGASNNGSNDLASYDAGFGLGWELDFWGRFRR
                     GIESADAAFFASLTNHQDAQVLLSAQVADLYYALLTTRMRIGIARANAEIQRRSLEI"
BASE COUNT          681 a         1340 c         1527 g          824 t
ORIGIN      
        1 ctcgaggaac gagaagagtt tagcgatcag acggaattcg tcgacgagga cgtctcggaa
       61 gacgacgctg aaatcgagcg gggtgacggc gagtttggtg acgacgaagc acttggcgac
      121 gatctcgacg actacgacga cgatgtcgga gcggttgacg acgagctcac cctgaaggaa
      181 cagaacgcca gatcgcttgc catccgtaga gccatcgagc agcgcctgga gcggaagcgt
      241 ctggacgaag acctcgatta cctcgacttc gatttcgatg aatgaggcca taaaagggtc
      301 ttagcccgta tactttcccg atgcagctca gtgctccgcc cgtcagggat ctggtgctgg
      361 tcggtggtgg ccacagccac gtccaggtac tcaagtcttt cggtatgcgc cccgtcccgg
      421 ggacgcggct caccttggtc agtcgtgagg tctacacgcc ttactccgga atgctccccg
      481 gctatgtggc cgggttctac gactggcacg acatccacat cgatctaggc gcccttgcgt
      541 cgtttgccgg agcgcgactg atcgtcgatg aagtgacagg agccgatctc gacgggggcc
      601 ggctgcgcct cgccgctcat cccgatctgc attacgacct gctctccata aatgtggggg
      661 cagtcccccg gggggtgggt caaggggtac cggtaaagcc catcggccgg tttctgcccc
      721 agctcgagcg ggttcgagaa gaagccgggc ccggtacgtc tatcgccgtc gttggcggag
      781 gggcgggcgg cgtggagctt gcgctggcaa tgagccgcac gatgccgcat ggggtcgagg
      841 tgaagctggt gaccgacgag ctgctgccgg gctacccggc cggcgtggtc cgacggctgg
      901 agaaggcggt gctccggtcg ggggtcgtgc tcatccgaga ctttcgtgcg ctggattgca
      961 ggcacgggct gctcgaaagc tccgatgggc ggtcgacggc ggcggaccat gcgttctggg
     1021 ttaccggggt gatggcagcg tcgtggccgg ctgcggctgg gctggccacg gatgtcagcg
     1081 ggttcatcga ggttgatcgc tgcctgcggt ccacgagtca tgagagcgtt tacgcggccg
     1141 gggacgtggc ctgtctaatc gatcagccga ggcccaagtc gggcgtgtac gccgttcggg
     1201 agggcccggc gctgacccgc aatctaaggc gggcaatcgt cggacggccc gggattccgt
     1261 tccgggcaca gaagcgcttc ttgagcctca tcggaactgc cgacgggtcc gctgtcgccg
     1321 ctcgcggccc ttgggcggcc agcggccgct gggcctggcg atggaaggat tggatcgacc
     1381 gccggttcgt ggcgcgcttc aaccggctgc cggtcatgac ggaaccgggc tggtcgctgc
     1441 cagagagtct ccgtgacgaa gcgccggacg ccatgcgatg cggaggctgc ggggcgaagc
     1501 tcggcgcgag cccgctgatg cgcgtgctcg agcgcctgcc gcctcaggtg tccgggaacg
     1561 tgcgggtcgg catcggcgat gacgcggcca tcctcggcgc gaccggtggc gagacgatac
     1621 tcaccgtcga cggcttccgc agcctcgtct ccgaccctta cctgtttggc cgcataacgg
     1681 cccaccacgc cttgaacgac atcatcgcca tgggcggtaa gggtacggct gccctagcga
     1741 tggcgagcgt gcctctgatg tccgaagcgc tcatggagga agatctcttt cagctgcttc
     1801 gcggcgcggt ggacgtgctc aatgcgcacg aggtgcccct ggtgggcggg cactcgagtg
     1861 agggagccga gctgagcctg ggtcttaccg ttgcgggcgt tcccgacgga ccgaccttga
     1921 ccaagggcgg ggcggaaccg ggggacgcgc tggtgctcac caagccgctc ggcacgggtt
     1981 cgatcctggc cgggcagatg cgcggcgccg ttcgctcgga cagcgtctcc gccgctattg
     2041 ccagcatgga cgcatcgaac gcatcggctc tggtcgtgtt ccgtcgctac ggcatccatg
     2101 ccgccaccga cgtgtcgggc ttcgggttgc tcggtcacct aggggagatg ctcagggcgt
     2161 ccgccctggg tgtgcggctc gacctcaacg cggtgccttt catcgagggt gcggtcgagg
     2221 ccgtcagcgc cggtgccgtc agctcgctgc aagacaacaa cgagctcgcc ctcgcggatt
     2281 tcgcgctgga aggctgcgag ccgggcgatg cgactgtgcg tctgctcgtc gaccctcaga
     2341 cttccggcgg gctgctcggg tgcgtgccgg aggcggaggc cgaagcctgc gttcgggatc
     2401 tgcgcgaggt cggttacccg cgtgcggccg tcataggccg ggtgctgtcg aacggctgga
     2461 ccgttaccgc cgaacgagag gcgcgcccgc tcagctgagc cgcgagacga ccatgcgcgt
     2521 cctcgcgtat gattcgccgt ttggcatcag ttgggggagt ggaaatgagc accgagcagg
     2581 acaccgacgt catccgcacc atcgtttccc aggacttgct ggacgtgctg gtccgggtgg
     2641 ggctgatcgg catccttctg gtgatgagct tccggatatt ttctccgttt ctcagcattc
     2701 tcctgtgggc gctcgtgctc gctgtgtcgc tgtacccggt gcatctgaaa cttgtcggca
     2761 agatgggtgg tcggcagggc gcgtcggcaa ccgtgatcgt cattgtcggg atgctgctga
     2821 tcggcacgcc gaccatagcc ctgggcagcc tttttgccgg ctatatccac gaaacctatc
     2881 aggcgtttgc gaccaactcg gtcaccgtca acccaccgag gccggaggtc gccgagtggc
     2941 ccatcatcgg ggagcggatg tatgcggcct ggtcggcggc ggcaagcgac atgcctgcct
     3001 tcctggagga attgcagccc cagctcggcg ttctgacgga tcgtgcgctg agcacggcgg
     3061 ccagcaccat gggcgatgtg ctgctctttc tcggtgccct catcatagcg ggcatcatga
     3121 tggcgttcgg ccagtcgggc agcgacgcca tgggtcggat cctcagtcgg ctgtccggcc
     3181 cggaaaacgg gccccgcctg cacaacctga cgacggcgac gattcgctcg gtggccacgg
     3241 gcgtgatcgg cgtggcgttc attcaggccc tgctcctcgg cgtgggattc gcctttgcgg
     3301 gcatcccagc cgcaggtttg ctggcgctgg tggtgatgct ggtcggcatc gtccagctgc
     3361 ccgcgctgct ggtcacgctt ccggtgatcg cctatctgtg gtggggcggc gatgcgtcca
     3421 cgaccagcaa cgtcatatgg acgatctacc tgctcgtagc tggtatggct gacaatgtcc
     3481 tcaaacccct gctgctgggt cgcggcgtcg acgcgcccat gccggtgata ctgatcggcg
     3541 cgctgggcgg catggtgtcc gccggcatcg tggggctgtt cgtcggcgcc gtgatcctgg
     3601 cggtgggcta ccagctcttc atggattggg tcaatggcgg cgccggagcc gaaacgtctc
     3661 cggaaggctc tgcagcagag ctagagggcg ggaccgtccc cgaaccccga gcctgataac
     3721 gtgttctcgc ccagcaagtc gccgacggcg tcggtgctgg tgctgctggg ggtactggcc
     3781 gcccaaggag cctgcaccgt cctcgggccc gattacgagg aacccgaggt gggatggctg
     3841 ggtgattggc agacggatct ctatggccgg ctcgaagagc ctgaagccgg cactcaatcg
     3901 gatctttctt tctggtggat gctgtttggc gaccccacgc taaacgcgct ggtggagact
     3961 gcgaaacgcg acaacatcgg attgcgtgtc gcgggtctgc gtattctgga gagtcgggcc
     4021 gtgctcggga tcgcgggcag cgcccgctac cctcaggtcc agcaggtaac cggtgcggta
     4081 acttacgtga ccagccgtga gcacggcggc gcatcgaaca acggatccaa cgatctcgcc
     4141 agctacgatg cgggcttcgg tctgggctgg gagcttgatt tctggggacg cttcaggcgc
     4201 gggatcgagt cggccgacgc cgccttcttc gcctccctga ccaaccacca ggacgctcag
     4261 gttctgctca gcgctcaggt tgccgacctg tactacgcgc tgctgaccac acggatgcga
     4321 atcgggatcg cgcgcgccaa tgcggagatt cagcgtcgca gcctggagat ca
//
LOCUS       DBNHKV010000403         4661 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1332004, whole genome shotgun sequence.
ACCESSION   DBNHKV010000403 DBNHKV010000000
VERSION     DBNHKV010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1332004"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="AC16PP_13110"
     CDS             <1..926
                     /locus_tag="AC16PP_13110"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR6482613.1"
                     /translation="ITQELFDSGQDSELDVQQARTQYLTTVSTIPELEIVEVKVLNAL
                     SALLGRPPGEVPELADMPDRLPGLDPSSIEAIPARLLTRRPDVRAAAWGVAAQSAQIG
                     IAQADYYPAIAVVGTVGWAGNSLSATPDVGTLAAGPALTWNVFDHGRIRNNVRVQDAR
                     LQQAIAGFQNTVLQAAREIDDAAITLVKTRERQATLADAVLSARRALDLARVRYREGY
                     SDFQRVLDAQRALFGAAERDLVNKGNHISAVIQFYKALGGGWTEMPIGELLPETVREK
                     MRTRSNWGDLLDAPLPVNQEGPVPAEGGTTP"
     gene            923..2062
                     /locus_tag="AC16PP_13115"
     CDS             923..2062
                     /locus_tag="AC16PP_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008041547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR6482614.1"
                     /translation="MSEQDHESEKAAEAQPESATAEKAVKRGGQMIGLVIVVTLVWYL
                     LADRYTPYTTQARVQGYVIGVAPKVSGLVTQVWAQNNREVQQGERLFEIDRSEYQIAV
                     GKARSDLENARRQVEAGRAAVEASRANLAAALANRVRAEKDYDRLQRLHSRDPGTIST
                     RRLEMSRASLDQAVAAVSAAESNVQAAIEQMGGEDEASNAILQSAQSAVARAELDLSN
                     TIVTASSRGVITDLRADVGQFAGAGSAVMTLIAVHDLWISAEFTENNLGHMRSGTEVE
                     ILFDVVPGRVFSGRIRSLGLGVSAGQSHPPGTLPTIQNNRDWLRQSQRFAVIVDFDPN
                     QHGELRSQLRIGGQASVMGLTEGHGVLNLLGQLYMRIMSWLSYAY"
     gene            2080..3117
                     /locus_tag="AC16PP_13120"
     CDS             2080..3117
                     /locus_tag="AC16PP_13120"
                     /inference="COORDINATES: protein motif:HMM:NF022616.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2955 domain-containing protein"
                     /protein_id="HIR6482615.1"
                     /translation="MPVQARRVFRLSGVMAVSLALSYAMQMDLPFIAPIFALLLTAAP
                     GPPKGLKALVGLSLVLAVTLGVGLLLVPLLLNYPVTAILIAGAGIYFSNYVSVNLGKG
                     AVGALLTAGVTLISAAGTLSFPLAVTVIEALVLGVGIAVISQWIVYPWFPENESFVPP
                     APPAATPSGSNWIALRATLVVLPAYLLALTNPAMYLPIIMKSVSLGQQSSVVDARDAG
                     RVLLGSTFLGGCFAIVFWFALGVATNLWTFTLLMLLVGVYFSGKLYQVIPTKFPAAFW
                     QNVVVTMLILLGSAVQDSDSGKDVYQAFAVRMSLFVAVTCYAWFAIEVLELLRSRRLG
                     RPEDALGVGGG"
     gene            3120..3323
                     /locus_tag="AC16PP_13125"
     CDS             3120..3323
                     /locus_tag="AC16PP_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482616.1"
                     /translation="MNLLYGLLVMLVLVAGNLAQVGIWGLLFMLLGPLQAIDGILMIG
                     VSTAVLMAAVGNVIRKTAGVDED"
     gene            3320..3958
                     /locus_tag="AC16PP_13130"
     CDS             3320..3958
                     /locus_tag="AC16PP_13130"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR6482617.1"
                     /translation="MTQASPVKIWLVTDPLCSWCWGMSQAVEAAVVRLEGEAEFDFLL
                     GGINVHGTQPIGEYGRRHLMRIWRDVQETTGQTFGFRLPDVLVYNSTAACMAVEAVRR
                     LTGKAPFGYLHRLQQLFFVEGRDINDPDLLAGIARDFGCDRNAFAALLDDPAVADHVR
                     DEFAGARLYGTNALPSVLWEGSGGRELLAGGYADADMLVELIRAKRNSQPPA"
     gene            complement(3930..>4661)
                     /locus_tag="AC16PP_13135"
     CDS             complement(3930..>4661)
                     /locus_tag="AC16PP_13135"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6482618.1"
                     /translation="EDVANGGAAVLDHLDVQAVNTVLGASMGGMTALAFALLHPDRAR
                     RLLSISSAARSLPFSIAVRSLQREIIRSDPGWKNGNYAFDEGPVTGMRLARKLGMISY
                     RSADEWAERFGRERSVDGQDADSPFGIDFEVESYLEAHAKKFVGTFDANCYLYLSRAM
                     DLFDATDHGGSLDSALKRVRLEQALVIGVETDFLFPVEQQEELALGLQRYVSDLSFRR
                     LPSIHGHDSFLVDMDRFRPAVASFF"
BASE COUNT          808 a         1442 c         1537 g          874 t
ORIGIN      
        1 agatcaccca ggagctcttc gatagtggtc aggattccga gctcgacgtg cagcaggcga
       61 gaacccagta cctgaccact gtgtccacga tcccggaact ggagatcgtc gaggtcaaag
      121 tcctgaacgc cttgtcggcg cttctcgggc gcccgccggg cgaggtgccc gaactcgccg
      181 acatgccgga taggttgcct ggcttggatc cctcgtcgat agaggcaatc ccggcgcggc
      241 tcttaacgcg ccggccggac gttcgcgcgg cggcctgggg ggtggcggca cagtctgcgc
      301 agatcggaat cgcccaggcc gactactatc ccgccattgc ggtggtggga accgtgggtt
      361 gggctggaaa cagcctcagc gccacgccgg atgtggggac cctcgctgcc ggccccgcgc
      421 tgacctggaa cgtctttgac catggtcgca tccgcaacaa cgttcgtgtt caggatgccc
      481 ggctgcagca ggcaatcgcc ggcttccaga acaccgtgct tcaggcggcc cgcgagatag
      541 acgatgcggc catcacgctc gtgaagacca gggagcggca ggcgacgctg gcggacgccg
      601 tgctgtcagc gcggcgggct ctcgatctgg cccgggtgcg ctaccgcgag ggctacagcg
      661 acttccagcg cgtgctggat gcccagcggg cgctgttcgg ggccgctgag cgggatctgg
      721 tcaacaaggg aaaccacatc agcgccgtca ttcagttcta caaggccctt gggggtggtt
      781 ggacggagat gcccatcggt gagctgctgc ccgagaccgt tcgggagaag atgcgcactc
      841 ggagtaattg gggtgacctg ctcgatgcgc cgctgcccgt gaatcaggag ggccctgtgc
      901 cggccgaagg agggacaacc ccatgagcga gcaagatcac gagtcggaga aggccgcgga
      961 agctcaaccg gaatcggcaa cggccgaaaa agccgtcaag cgtggcggtc aaatgatcgg
     1021 tctggtgatc gtcgtcaccc tcgtctggta cctcctggca gaccgctaca cgccatatac
     1081 cacccaggca agggtgcagg gctacgtcat cggggtggca ccgaaggtgt ccggcctcgt
     1141 gacccaggtg tgggcgcaga acaatcgcga ggtccagcaa ggggaacgac tgttcgagat
     1201 cgaccgctct gagtaccaga ttgccgtggg caaggcgcgc tcggacctgg aaaacgcccg
     1261 cagacaggta gaggccggac gcgccgcggt agaggcttct cgcgccaacc tcgccgctgc
     1321 cctcgccaat cgggtcaggg cggagaagga ctacgatcgt cttcagcgac ttcactcccg
     1381 ggatcccggg acgatctcca cccggcggct ggaaatgtcc agagcctcgc tcgaccaggc
     1441 ggtagccgcg gtctcggcgg ccgagtcgaa tgtccaagct gccatcgagc agatgggggg
     1501 cgaggacgaa gcctccaacg ccattctgca atccgcacag agcgcggtcg cgcgcgcgga
     1561 gctggatctt tccaatacca tcgtcacggc ttcttcccgc ggcgtgatca cggatcttcg
     1621 cgcggacgtc ggccagttcg ccggtgcggg cagcgcggtc atgacgctca tcgcggtgca
     1681 cgacctttgg atcagcgccg agttcacgga gaacaacctg gggcatatgc gaagcggcac
     1741 cgaggtggag atcctcttcg acgtggtgcc gggcagagtc ttttccggca ggatacgcag
     1801 cctcggcctc ggagtgagcg ccggccagtc tcatccgccg gggacgctgc ctaccattca
     1861 gaacaaccgg gactggctga gacagtccca gcgctttgcc gtcatcgtgg acttcgatcc
     1921 gaaccagcac ggcgaacttc gctcgcagct gcgcatcggg ggccaggcgt cggtgatggg
     1981 cctcactgaa gggcacgggg tgctgaacct gctgggtcag ctctacatgc gcatcatgag
     2041 ctggctgtcc tatgcttact gatcgggaag gcctctgaaa tgccggtcca ggctcgacga
     2101 gtcttccggc tgagcggcgt gatggccgtc tctctggcgc tcagctacgc catgcagatg
     2161 gatctgccgt tcatcgcgcc gatttttgcg ctgctgctca cggcggctcc gggcccgccc
     2221 aagggcctga aagccctggt cgggctttcc ctggtgctgg cagttacgct gggcgtcggg
     2281 ctgctgctcg ttcccttgct gctcaactat cccgtcaccg ccatcctgat cgcgggagcg
     2341 ggcatctact tcagcaacta tgtgagcgtc aatctgggca agggtgccgt cggggcgctt
     2401 ttgacggcgg gcgtcaccct gatatcggcg gcgggtacgc tgagttttcc gctggcggtg
     2461 accgtgatcg aagcgctggt gttgggtgtt ggcattgcag tcatcagcca gtggatcgtc
     2521 tacccctggt ttccggaaaa cgagtcgttc gtacctccgg caccgccggc tgcgactccg
     2581 agcgggagca actggattgc cctgcgagca accctcgtcg tgctgcccgc ctacctgctc
     2641 gcgctcacca atcccgccat gtacctgccg atcatcatga aatcggtctc tcttggtcag
     2701 cagagctcgg tcgtcgatgc ccgcgacgcg ggccgggtgc tgctcggatc gacgtttctc
     2761 ggtggctgct ttgccatcgt gttctggttt gccctcggcg tggcgacgaa cctgtggacg
     2821 ttcacactgc tgatgctgct ggtgggcgtg tacttctccg gcaagctcta tcaggtcatc
     2881 ccgacgaagt ttccagccgc gttctggcag aacgtggtgg tgacgatgct gatcctgctt
     2941 ggctccgcgg ttcaggacag cgattccgga aaggacgtct accaagcctt tgccgtgcgt
     3001 atgagcctgt tcgtggcggt tacctgctac gcgtggtttg caattgaagt gctcgagctg
     3061 cttcgatctc gacgactcgg tcggcccgag gatgcgttgg gcgtaggagg cggctgaaaa
     3121 tgaacctgct ctacggtctt ctcgtgatgc tcgtgctggt tgcgggcaac ctcgcccagg
     3181 tgggaatctg ggggctgctc ttcatgctcc tcggacccct tcaagccatc gacggaattc
     3241 tgatgatcgg ggtctctacg gccgtgctga tggccgccgt tggtaacgtg atcaggaaga
     3301 ccgcgggggt cgacgaggat tgactcaggc atcaccggtc aagatatggc tcgtcaccga
     3361 ccccctgtgc tcgtggtgct ggggcatgtc ccaagcggtc gaagcagcgg tcgtgcgcct
     3421 ggagggcgaa gccgagttcg acttcctgct cggcgggatc aacgtgcacg gcacccagcc
     3481 gatcggcgag tacggtcgtc gtcatctgat gcggatctgg cgcgacgttc aggagaccac
     3541 cggtcagacc tttggttttc gcctgccgga cgtcctcgtt tacaacagca cggctgcctg
     3601 catggccgtc gaggcggtga ggcgcttgac cggcaaggca ccgttcggat atctgcaccg
     3661 cctccagcag ttgttcttcg tggagggccg cgacatcaac gatccagacc tgctcgcggg
     3721 gatcgcgcgc gacttcggtt gcgaccgaaa cgcattcgct gcgctgctgg acgacccggc
     3781 agtggccgac cacgttcgtg acgaatttgc cggcgcgcgc ctgtatggca ccaacgcgct
     3841 gcccagtgtg ctgtgggaag gaagcggtgg ccgggaacta ctggcgggcg gttacgcgga
     3901 tgctgacatg ctggtggagc tcattcgtgc taaaagaaac tcgcaaccgc cggcctgaag
     3961 cggtccatgt cgaccagaaa cgaatcgtgt ccgtggatgc tcggcaggcg tcgaaaggac
     4021 aggtcgctta cgtaacgttg cagccccagc gcgagttcct cctgctgctc gacgggaaag
     4081 aggaagtcgg tctccacccc gatcaccagg gcctgttcca gcctgacccg tttcagtgcg
     4141 ctgtcgagac tgccaccatg atccgtggcg tcgaaaagat ccatcgcccg ggaaagatag
     4201 agatagcagt tggcatcgaa cgtgccgacg aacttcttcg cgtgagcctc cagatacgat
     4261 tcgacctcga agtctatgcc gaaggggctg tcggcatcct gcccgtcgac gctgcgctcg
     4321 cggccaaagc gctctgccca ctcgtcggcc gatcggtagg agatcatgcc aagcttgcgg
     4381 gccagccgca tgcccgtcac cggcccttcg tcgaaagcgt agttgccgtt cttccagcca
     4441 ggatcgctgc gaatgatctc gcgctgaagg gaacggacgg cgatggaaaa cggcaggctg
     4501 cgcgctgctg acgagatgga gaggagccgg cgcgcccgat caggatggag cagggcgaac
     4561 gccagcgccg tcatcccgcc catggaggcc cccaagaccg tgttcaccgc ctgaacgtcc
     4621 aggtggtcga gaacggcggc cccgccgttg gcgacgtcct c
//
LOCUS       DBNHKV010000404         2616 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1401698, whole genome shotgun sequence.
ACCESSION   DBNHKV010000404 DBNHKV010000000
VERSION     DBNHKV010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1401698"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..989
                     /locus_tag="AC16PP_13140"
     CDS             186..989
                     /locus_tag="AC16PP_13140"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR6482619.1"
                     /translation="MNHLFFFGSCVAVGALSGFLGGLLGIGGGIVIVPALIILFDTME
                     LFPQSIATPVAVGTSLAVIIFTSLAAARTQIRAGMVDWDVVRRWAPFLIIGSFLAGPI
                     AVALPVATFRTLIGAFLLFVSCVMLTNWKPDPHRKFPGAVAIAGIGMLGGLISGIAGV
                     GGGNVMVPTLVYFNKPVHRATATSSTLGVPIATAGTLGFVYTGWNAELGPALLGFVYL
                     PAFVAIVLATVVTAPMGVRVAYRVAPLPLRRGFGLLLVVIAARTLYSAF"
     gene            complement(1016..2419)
                     /gene="pyk"
                     /locus_tag="AC16PP_13145"
     CDS             complement(1016..2419)
                     /gene="pyk"
                     /locus_tag="AC16PP_13145"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR6482620.1"
                     /translation="MRRRRTKIICTIGPATANYPTLERLQAAGMNIVRLNMSHADHAS
                     AKKIINWIRTLNRKVQYPVAILLDTQGPEIRTGEINEPMDLSTGDVVTISVLPEDVER
                     SSIHVNYRELVDILQVGNRMTVDNGLLNFEVLEKKGQQLVCRVIDGGVLRSKSHVNLP
                     GVRVNLPSITPKDRRDVEFGIENDVDFIALSFVRSAEDVTELRGILGAKAKNIKIIAK
                     IEDQEGVTHVHDIVRAADGVMVARGDLGIETNIADLPNVQRRIVHSAAKWGKRSIVAT
                     HLLESMIENPIPTRAEVTDVANAIYEGVDAIMLSGETSVGKHPVRCVEQLDDIARSSE
                     RWPGLGYEKELVANTDKQHIANNAVALAEDIHAKGIVVITRRGITADLVTSARPRNVP
                     IYAFTNHSQTRRRMSLNRAVFGHRMQFSSDPETTLQRALKILRDREGFEPGDKVVVIS
                     DVLAEKNSDAIQLRHLD"
     gene            complement(2419..>2616)
                     /locus_tag="AC16PP_13150"
     CDS             complement(2419..>2616)
                     /locus_tag="AC16PP_13150"
                     /inference="COORDINATES: protein motif:HMM:NF024703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HIR6482621.1"
                     /translation="QMAGRLLRDPEDRGILCLVDDRFKDSAYGRFFPAHWRPQAVRAA
                     QVAGRLEKFWQGAAGSPRLRA"
BASE COUNT          394 a          842 c          826 g          554 t
ORIGIN      
        1 cgaccacctc tccttcgtaa aaatcaccgt tggcgagctc gatggtttcg gtacgggcaa
       61 gcgcctggct gcccagaacc agcgccggga aaagcatggt gaatgcgagc cgccgtgcgg
      121 cggtggaatg gtgagctacc tgcaagttca gtagactccc gagatcgtcg agtacgttac
      181 tttaattgaa tcatcttttc ttctttggtt cctgcgtcgc agttggcgcc ttgagcggct
      241 ttctcggcgg tctcctgggc atcggcggcg gcatcgtcat cgtccccgcc ctcatcatcc
      301 tgttcgatac catggagctc tttcctcaga gcattgccac gcctgtggcc gtgggaacct
      361 ccctcgccgt gatcatcttc acgtccctcg cagcggcgcg aacccagata cgggctggca
      421 tggtggactg ggacgtggtc cggcgatggg cgccctttct gatcatcggc agcttccttg
      481 cgggacccat cgcggtcgcc cttccggtgg ccaccttccg caccctgatc ggggcatttc
      541 tcctcttcgt atcctgcgtg atgctcacga actggaagcc ggatccgcac cggaaatttc
      601 ccggggccgt ggcgatcgcg ggcatcggca tgctgggcgg cctcatctcc gggattgccg
      661 gtgtcggcgg ggggaacgtg atggtgccca cgctcgtcta cttcaacaaa cccgtgcaca
      721 gggccaccgc gacgtcgagc accctcggcg tgcccatcgc caccgccggc accctgggtt
      781 tcgtctacac cggctggaat gccgagcttg gccccgccct gctcggcttc gtgtatctgc
      841 cggcgttcgt ggccatcgtg ctcgccaccg tcgtgacggc gccaatgggc gtgcgcgtcg
      901 cctatcgggt cgcgccactg ccattgcgca gaggattcgg cctgcttctg gtggtcatcg
      961 cggcgcggac gctctactcg gcgttctgac acctgccagc ggcggccgtc gggattcagt
     1021 ccaggtgccg cagctgaatg gcgtcggagt ttttctcggc cagcacgtcc gagatgacga
     1081 cgaccttgtc gccaggctcg aacccctcac gatcccgcag gattttcagc gccctctgca
     1141 gcgtggtctc gggatcgctg gagaactgca tgcgatggcc gaagacggcc cgattcagcg
     1201 acatgcggcg ccgcgtctgg ctgtggttgg tgaacgcgta gatgggcacg tttcgcggcc
     1261 gcgcgctcgt gacgagatcg gcggtgatgc cgcgccgggt aatgaccacg atgcctttgg
     1321 catgaatgtc ctcggccagc gctaccgcgt tgttggcgat gtgctgcttg tcggtgttgg
     1381 ccacgagttc cttttcgtat ccgagcccgg gccaacgctc tgatgaacgg gcgatgtcgt
     1441 cgagctgctc cacgcagcgc accgggtgct tgcccacgct ggtctcgccg gagagcatga
     1501 tggcatcgac gccctcgtag atggcgttgg cgacgtcggt cacctcggcc cgggtgggaa
     1561 tggggttttc gatcatcgac tccagcaggt gcgtcgccac gatggagcgt ttgccccact
     1621 ttgccgccga gtgcacgatg cgccgctgca cgttcggcag atcggcgatg ttcgtctcga
     1681 tgccgagatc gcccctggcc accatcaccc catcggccgc gcgcacgatg tcgtgcacat
     1741 gggtcacccc ttcctgatct tcgatcttcg cgatgatctt tatgttcttc gccttcgcgc
     1801 ccaggatgcc gcgcagctcg gtgacgtcct ccgccgagcg cacgaaggac agcgcgatga
     1861 agtccacgtc gttctcgatg ccgaactcca cgtcccggcg atccttgggc gtgatcgatg
     1921 gcaggttcac acgcaccccc ggcaggttga cgtgagattt gctccgcaga acgcccccgt
     1981 ctatcacccg gcagacgagc tgctgacctt tcttctccag cacctcgaag ttgagcagac
     2041 cgttgtcgac ggtcatccgg ttgcccacct gcaaaatgtc cacgagttcc cgatagttga
     2101 cgtgaatgga gctgcgctcg acgtcctcgg gcagcacgct gatggtcacg acgtcgccgg
     2161 tgctcagatc catgggttcg ttgatctcgc cggtgcggat ctcggggccc tgggtatcga
     2221 gcaggatggc gaccggatac tgcaccttgc ggttgagggt gcggatccag ttgatgatct
     2281 tctttgccga ggcgtggtcc gcatgggaca tgttcagacg gacgatgttc attcccgccg
     2341 cctgcagcct ttccagcgtg gggtagttgg ccgtggctgg accgatggtg cagatgatct
     2401 tggtacgtcg tcgtctcatc acgcgcgcag tctaggggag ccggccgccc cctgccagaa
     2461 cttctcgagg cggccggcga cctgggcggc cctcaccgct tgggggcgcc agtgagcggg
     2521 aaagaatcgg ccgtaggccg aatctttgaa gcggtcgtct accaggcaga gaatgccccg
     2581 gtcttccgga tcacgcagca ggcgtccggc catctg
//
LOCUS       DBNHKV010000405         9669 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_695075, whole genome shotgun sequence.
ACCESSION   DBNHKV010000405 DBNHKV010000000
VERSION     DBNHKV010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9669
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_695075"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="AC16PP_13155"
     CDS             <1..427
                     /locus_tag="AC16PP_13155"
                     /inference="COORDINATES: protein motif:HMM:NF044732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6482622.1"
                     /translation="TEPEANPLKDWMESHDRFLRVPDDTLVLPAHNLPFYGVRERLRQ
                     LIDHHEDRMLAIEEHCVEPAVAKDLLPVLFARELDPRQMMMALGEAIAHLHLLVHRNR
                     IERTLHEDGCYRFLSIDPDLKRRAHPGDHDAPSERPVLV"
     gene            453..731
                     /locus_tag="AC16PP_13160"
     CDS             453..731
                     /locus_tag="AC16PP_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482623.1"
                     /translation="MQKGKLKRRIADYTYRMMHWGQNRLPPGIRSLVGVLLMVGGVFG
                     FLPVLGFWMLPLGVAFVALDVPGTRRHIDMWMLRLYRQAYLDEPTSNQ"
     gene            complement(701..1567)
                     /locus_tag="AC16PP_13165"
     CDS             complement(701..1567)
                     /locus_tag="AC16PP_13165"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6482624.1"
                     /translation="MNHWMVGDVKITRVVEMEVAGGTRFILPDATRDACLPIEWLHPH
                     FMDEAGNLIMSIHALVVDTGSRRIIVDTCIGNDKQRSIPAWTNLQTSFLEDLGAAGYP
                     RETVDTVLCTHLHVDHVGWNTMLVDGEWVPTFPNARYLVAEKEFRYWDGEAEDEINQG
                     IMADSVRPIVAAGLMDFVDWEHQLCPEVALEPTPGHTPGHVSVHIRSNGKEALITGDC
                     IHHPCQMTRTDWCSSADYDRAAGEATRERLLESYVDSDVLIIGTHFATPTAGHVRRLD
                     GGGYWLDVGSSR"
     gene            1689..3509
                     /locus_tag="AC16PP_13170"
     CDS             1689..3509
                     /locus_tag="AC16PP_13170"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HIR6482625.1"
                     /translation="MGPIAKAVDVVRALSNVPTLLALKRGMKPRPVDEHDCFAANVER
                     NAAAFPDRTAIIFEGNSVTWAEFNAQCNRFASAFKAQGLRKGDAVSVMMENRIELLAT
                     IVALNKLGVVAGLINTNLRGRPLAHCITVTESRTCVFGEEVGEAVAEVKDELPLEEGS
                     GYLFVPDAGNGAAPNWAADLSRLAADADDADPPETGDVQLGDRALYIFTSGTTGLPKA
                     AVLSNRRFVATAALVHKAGLRCSEEDRIYICLPLYHGTGLLIGVGASFSSGASMFIRR
                     KFSASNFLREVREYQTSCLIYIGELCRYLMNTPAQPDDHDNPLVTMMGNGLRPDVWPA
                     FKSRFGIKRITEFYGASEGNVSFVNLLNKDCTIGMTGSKVALVRYDVDEDEIVRDENG
                     HCMEVAPGEPGLLLGHISEDAVFEGYTNAEATESKVVRNAFEDGDAWFNSGDLIRQVD
                     VGYSLGYPHYQFVDRVGDTFRWKSENVSTNEVGELINGFPDVKFCNVYGVEIPGADGR
                     AGMVAITLADGVTELDADALSRHVSDTLPPYARPVFVRVQPDIDVTGTFKMVKGDLRK
                     QGYDLDQITDPLFVMKPGESAYVPLDREFAGKIASGAAGY"
     gene            complement(3517..4581)
                     /locus_tag="AC16PP_13175"
     CDS             complement(3517..4581)
                     /locus_tag="AC16PP_13175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6482626.1"
                     /translation="MELWTLTHSRADNVLAVARRAEAAGWAGVMVVDSQNLAADSYVG
                     LAVAATGTQRIGLGTGVTNTVTRHPAVTASAAMAVQDVSGGRMVLGIGRGDSALAHLG
                     RSPARLPGFERYLDVLQRYLKGEAVPFEDIPMDDHIAAPVADLDLADHPSASRIRWFK
                     DDVAKVPVEVAATGPKVIEMAARLADRVMFTLGAVEERLSWAMETAMAARTGAGIPDD
                     PAFGAYVNLVCHRDRAVARDLVRGGLTTFARFSVMHGQIAGPADDHDRATLTSLHDGY
                     DMKAHTRADSSQAALLDDDFVDRFAIAGPPDYCVERLNRLASMGIEKVAISGPTAGTD
                     PVEARRALALLDEAVVPQLA"
     gene            4908..5528
                     /locus_tag="AC16PP_13180"
     CDS             4908..5528
                     /locus_tag="AC16PP_13180"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR6482627.1"
                     /translation="MKRALLLDLGNVVLRVDFRRTFDYWAEAARVPAERLYRRWQLDE
                     AYELHEVGALAFEEYIAALSRRLMIDLPMEEWRAGWNAVFAGAFQDVQARLPTVRQSM
                     PVYLFTNTNATHQAAWSHLYADTLSHFDALYVSSEIGRRKPNVSAFRHVAEDMGYAPE
                     QIVFVDDTKENVLGARAAGMDARMVGSEADVVRILDELTGTEPGDS"
     gene            complement(5523..6395)
                     /locus_tag="AC16PP_13185"
     CDS             complement(5523..6395)
                     /locus_tag="AC16PP_13185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6482628.1"
                     /translation="MTDFGVLIFPTAYSISPSDLARAAEARGFESIFFPEHTHIPACR
                     TTPWPGGRELPREYWNTYDPYVGLAMAAAVTKTLKVGTGITLVTERDPIVLAKQVASL
                     DRLSGGRVLLGIGAGWNVEEMRHHGVAFDERWAVLRERVLAMRAIWTEQAPEFHGEHV
                     DFEPLWSEPKPLQAGGPKILMGASSRWTWARIAEYADGWFPIHQDPARASAQGAVDYP
                     QGIQATREAWQAAGRQGEPDFSIFGMGPDRGRTEEMIRLGFNRVIFGLPSADADTVLP
                     LLDRYAEIGHAVNS"
     gene            complement(6411..7991)
                     /locus_tag="AC16PP_13190"
     CDS             complement(6411..7991)
                     /locus_tag="AC16PP_13190"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR6482629.1"
                     /translation="MLEVPVLIVGGGPVGLAAALECAYFGVPAMLVERHSGTTWHPKA
                     RNLNSRTMEIMRRLGLEPELQAVGLPEAWTRQIVYADTLSGAELGRMGTGGFESRSSA
                     ASPCAAVLSSQDVFEPIWRGRAEQQPLLDVRYGHQAEILDVDAAGAVVRLTGARGGDV
                     RARYLLAADGAGGAIRQGLGIGLEGPENLGHFINVYYRANLDRVVAHRPAVLYFTANE
                     AGRGVFQPLDGRKRWLSQIAYDGAADTRAGFDGKRCAQWIRNAAGEPDLKVEILGISH
                     WTMNATVAERYRQGAAFLVGDAAHQLPPTGGFGLNTGIQDVHNLVWKLAYDLRDWSDA
                     GLVDTYEAERKPVARFNADRAMDNSRAVARIQRAVADGEAAAGEVARSRRYGNFRGLE
                     LGYSYRSAAVIDDGSPAPEPEDAVEDYVPCARPGHRMPHMWLEGGALSTLDLVQRRFL
                     VLSDSARWLEAASQHNADCRVLPGNGGFLQLFGLEPGGAVLVRPDGHVAARFAEPPGF
                     PGGALASALRDLLNTTTT"
     gene            complement(8060..8713)
                     /locus_tag="AC16PP_13195"
     CDS             complement(8060..8713)
                     /locus_tag="AC16PP_13195"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR6482630.1"
                     /translation="MPTVATARQNENRKEAKRAKVRWYTPFLFVLHSLAIVGPVLLAA
                     GFAHRVEDAAYRTFAFAVPAPENSYVTWNSEVSEFGTKVSQAFGVRPSTATEFAGWIL
                     EASTRQELEPELLASLVLTESSFRKNVRSHVGAIGPAQVRPEYWSNFCGIANLHDPAE
                     NIYCGAQVLSHLRDRCGDEGCALHAYNVGLYAPRHKAGHRYVAKIDHYREQLLNLPL"
     gene            complement(8971..>9669)
                     /locus_tag="AC16PP_13200"
     CDS             complement(8971..>9669)
                     /locus_tag="AC16PP_13200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6482631.1"
                     /translation="WSHPAARMREFILAMRAIWSNWHEGEPLAFTGKFYTHTLMTPFF
                     TPTDTEYGPPRVFLAAVGPMMTEVAGEVADGVIVHAFTTEKYLRETTLPALERGFAKA
                     GKTRADFEISYPVFVVTGENEEELAQAKVATARQIAFYGSTPAYKPVLESIGVGEMQG
                     ELNAMSKQGRWEEMGHLISDDVLEAFAVVGEPQQIAGQIISRYGDIVDRTSAAYAHIS
                     KEHRQEIIAELRLA"
BASE COUNT         1706 a         3262 c         3067 g         1634 t
ORIGIN      
        1 caccgagcca gaggcgaacc cgctgaagga ctggatggaa tcccacgacc gattcctgcg
       61 ggtccccgac gacaccctcg tcctgccggc tcacaacctg ccgttttacg gcgttcggga
      121 gcggctgcgc cagctgatcg accaccacga ggatcgcatg ctggccatcg aggaacactg
      181 cgtcgagccc gcggtggcga aggacctgct tcccgttcta ttcgcacgcg agctcgaccc
      241 ccgtcagatg atgatggccc tgggcgaagc catcgcccat ctgcacctgc tggtgcatcg
      301 caaccgcatc gagcggacgc tgcacgagga cggctgctac cgcttcctgt ccatcgaccc
      361 ggacctgaaa cggcgagcgc atcccgggga ccacgatgcc ccgagcgagc ggcccgtgct
      421 ggtctgagcc ggaagagcgg agccacacgg cattgcagaa gggcaaactc aaacggcgaa
      481 ttgccgacta cacctaccgc atgatgcatt ggggtcagaa ccgcctgccc ccggggatcc
      541 gctctctggt gggcgtgctc ttaatggtcg gcggcgtttt cggttttcta cccgtgctcg
      601 gcttctggat gctgccgctg ggcgtcgcct tcgtcgccct cgacgttccc ggcacgcgac
      661 gccacatcga catgtggatg ctgcgcctgt accgccaggc ctacctggac gagcctacgt
      721 ccaaccagta acccccgcca tccaggcgcc ggacgtgacc ggcggtcggc gtcgcaaagt
      781 gggtcccgat gatcaggacg tcggagtcca cgtaggattc caggagccgc tcccgggtcg
      841 cttcaccagc agcacggtcg tagtcggccg aggagcacca gtcggtccgc gtcatctggc
      901 agggatggtg gatgcagtca ccggtgatga gcgcctcctt gccgttggat cgaatgtgca
      961 cgctgacgtg cccgggcgta tgaccgggcg tcggctccag agcgacctcg ggacacagct
     1021 gatgctccca gtccacgaag tccatcaggc cggcggccac gatgggccgc acggaatctg
     1081 ccatgatccc ctggttgatc tcgtcctcgg cttcgccgtc ccagtagcgg aactccttct
     1141 cggccaccag gtagcgggcg ttcgggaacg tgggcaccca ctcaccgtcc acgagcatgg
     1201 tgttccagcc cacgtgatcc acgtgcagat gcgtgcacag cacggtgtcc acggtttcgc
     1261 gcggatagcc cgcagcgccg agatcttcga gaaagctggt ctgaaggttg gtccaggcag
     1321 gtattgaccg ctgcttgtcg ttgccgatgc aggtgtcgac gatgatgcgg cgcgagcccg
     1381 tatccacgac gagcgcgtgg atgctcatga tcaggttccc cgcttcgtcc atgaagtgcg
     1441 ggtgaagcca ctcgatgggc aggcaggcat ctctggtcgc atccggcaga atgaagcggg
     1501 tgccaccggc cacctccatt tccaccacac gcgtgatctt cacatcaccc accatccaat
     1561 ggttcatcgc cgctccttgt ctgcccgggc cgaactgagc ctggttgagc cccgagcatg
     1621 ccacaggtcg cttgatggtg gcatacccgc aggataaggt gccccacgta gccgaacgag
     1681 gagaaagcat gggcccgatc gccaaggccg tggacgtcgt ccgggcgctg agtaacgtgc
     1741 ctacgctgct tgctctcaag cgcggcatga agccgaggcc cgtggacgag cacgactgct
     1801 tcgccgcgaa cgtggagcgc aacgccgccg cgttcccgga cagaaccgcc atcatcttcg
     1861 aaggcaactc ggtaacctgg gccgagttca acgctcagtg caaccgcttt gcgtcggcat
     1921 tcaaggctca gggcctccgc aaaggcgacg cggtcagcgt gatgatggag aaccgcatcg
     1981 aactgctggc gaccatcgtg gcgctcaaca agctgggcgt ggtggccggc ctgatcaaca
     2041 ccaacctacg cggccgaccc ctggctcact gcattacggt gacggagtcg cggacgtgcg
     2101 tgtttggaga agaggtgggc gaagccgtcg ctgaagtgaa ggacgagttg cccctggaag
     2161 agggctccgg ctacctgttc gttccggatg caggtaacgg cgcggcacca aactgggcgg
     2221 ccgatctcag ccgccttgcg gccgacgccg acgacgccga ccccccggag acgggcgacg
     2281 tgcagcttgg cgaccgggct ctgtacatct ttacctccgg caccaccgga ttgccaaaag
     2341 cggccgtact ctccaatcgc cggttcgtcg ccaccgccgc gctcgtccac aaagccgggc
     2401 tgcgctgcag cgaggaggac aggatctata tctgtcttcc cctctaccac ggcacggggc
     2461 tgctgatcgg cgtaggcgct tcgttctcgt ccggcgcaag catgttcatt cgtcgaaagt
     2521 tctcggccag caactttctg cgcgaggtcc gcgagtatca gaccagctgc ctgatctaca
     2581 tcggcgagct gtgtcgctat ctaatgaaca cgcccgccca gcccgacgat cacgacaacc
     2641 cgctggtcac catgatgggc aacggcctgc ggcccgacgt ctggcccgca ttcaaatccc
     2701 gcttcgggat caagcgcatc accgagttct acggcgccag cgagggcaac gtctcgttcg
     2761 tgaacctgct gaacaaggac tgcaccatcg gcatgacggg atccaaggtt gcgctcgtcc
     2821 gctacgacgt ggacgaggac gagatcgtcc gggacgagaa cggccactgc atggaggtgg
     2881 cgccgggcga gcccgggctg ctgctcggcc acatcagtga ggacgccgtt ttcgagggct
     2941 ataccaacgc ggaagcgacg gaaagcaagg tcgttcgcaa cgccttcgag gacggcgacg
     3001 cctggttcaa ctcgggcgat ctcatccgcc aggtagacgt aggctacagc ctgggctatc
     3061 cccactacca gttcgtggac cgcgtggggg acaccttccg ctggaagtcc gagaacgtct
     3121 ccaccaacga ggtgggcgag ctgatcaacg gtttccccga cgtgaagttc tgcaacgttt
     3181 acggcgtcga gattccgggg gcggacggcc gtgccggcat ggtcgccatc accctggcgg
     3241 acggcgtgac agaactcgac gcggacgccc tgtcccgtca cgtgagcgac acgctgcccc
     3301 cctacgccag gcccgtcttc gtcagggtgc agccggacat cgacgtcacc ggcacattca
     3361 aaatggtcaa gggcgatctg cgcaagcagg gttacgacct ggaccagatc actgatccgc
     3421 tgttcgtcat gaagccgggt gaaagtgcct acgtgcccct ggaccgggaa ttcgccggga
     3481 agatcgcttc aggcgcagcc ggctactgag gcgcgttcac gcgagctgag gcacaaccgc
     3541 ttcatcgagc agcgccagtg cccggcgcgc ctcaaccgga tcggtgccgg cggtggggcc
     3601 gctgatcgcg accttctcga tgcccatcga cgcgagccgg ttcaggcgct cgacgcagta
     3661 atcgggaggg cccgcgatgg cgaaacgatc gacgaagtcg tcgtccagca gagctgcctg
     3721 gctcgagtcc gcccgcgtgt gcgctttcat gtcatagccg tcgtgcaggc tggtcaacgt
     3781 tgcccggtca tgatcatcgg cgggaccggc aatctggccg tgcatgacgg aaaatcgtgc
     3841 gaaggtcgtg agcccgcccc ggaccaggtc ccgggcaacc gcgcggtcac gatggcaaac
     3901 cagattcacg tacgcgccga atgccggatc atcgggaatg ccggcgcccg ttctggccgc
     3961 catggccgtt tccattgccc agctcagtcg ctcctccacc gcgccaaggg tgaacatcac
     4021 acggtcggcg agccgggcag ccatctcgat caccttgggc cccgtggccg caacttccac
     4081 cggcaccttg gcgacgtcat ccttgaacca ccggatgcgg ctggcgctcg ggtgatcggc
     4141 aagatcgaga tcggcaaccg gagcggcgat gtggtcgtcc atgggaatgt cttcgaacgg
     4201 cacggcttcg cccttcaggt agcgctgcag cacgtccaga tagcgttcga acccgggcag
     4261 tctagccggc gatctgccca gatgcgccag cgcggagtcc ccgcgcccga tgcccagaac
     4321 catgcgcccg ccggagacgt cctgaacggc catggcggca gaggcggtca cggcgggatg
     4381 tcgcgtgacc gtgttcgtga ctccggttcc caggccgatc cgctgtgtcc cggtggctgc
     4441 cacggccagg cccacgtagg agtcggccgc caggttctgc gagtccacca ccatcacgcc
     4501 ggcccaaccg gccgcctctg cgcggcgggc gacggccagc acgttgtccg cacgggagtg
     4561 agtcagggtc cacagttcca ttggcggggc atccatcgtc gatggttctc caccatgatg
     4621 gttcaacccc tggaaaaagg gaaaccccgg gctggcgccc ggggccgtcc gcctacagcc
     4681 agggggaagg gagggagcat gggcccgctg ggggcatgcc gtagcgaact gggcaccggc
     4741 cgaataccat gcgggtcctg acgactgcgc acctccgtgc cggacctgcc aggcaacgac
     4801 cgagaccttg aggcagacct tagagccgat tcgctgaacg ccaactgaat gcagaaagac
     4861 ccgaaaatac ggcggcgccc accaccggcg accatccggg ccgggcgatg aagcgcgccc
     4921 tgctgctgga tctgggcaac gtcgtcctgc gcgtggactt tcggcgtacc ttcgactact
     4981 gggccgaagc ggcccgcgtg cccgctgagc gcctttaccg ccgctggcag ctggatgagg
     5041 catacgagct gcacgaggtg ggagccctcg ccttcgaaga gtacatcgcc gcgttgagcc
     5101 gccggctcat gatcgacctg cccatggaag agtggcgcgc cggctggaac gccgtcttcg
     5161 ccggcgcttt tcaggacgtc caggcgcgtc tgcccaccgt gcggcagagc atgcccgtgt
     5221 acctgttcac caacaccaac gccacgcacc aggccgcctg gagccatctt tatgcagaca
     5281 ccctgagcca cttcgacgcg ctctacgtct cgtcggagat cggccgccgc aagccgaacg
     5341 tctctgcgtt ccgccacgtg gccgaggaca tgggctacgc gcccgagcag atcgtcttcg
     5401 tagacgacac caaggagaac gtgctcggcg cccgggccgc gggcatggac gcgcgcatgg
     5461 tcggcagcga agccgacgtc gtgcgcattc tcgacgagct caccggcacg gagcccgggg
     5521 attcatgagt tgacggcgtg cccgatctcg gcgtagcggt cgagcagcgg caacaccgta
     5581 tccgcgtccg ccgagggcaa cccgaatatc acccggttga aacccagccg gatcatctct
     5641 tcggtgcgcc cccggtcagg ccccattccg aagatgctga agtcgggctc cccctgccgg
     5701 ccggctgcct gccacgcctc gcgggtggcc tgtatcccct gcgggtagtc cacggctccc
     5761 tgggccgacg cccgggcggg atcctggtga atggggaacc agccgtccgc gtactctgcg
     5821 atccgcgccc acgtccaccg ggaggaagcg cccatcagga tcttcgggcc acccgcctgc
     5881 agcggcttcg gctcggacca cagaggctcg aagtcgacgt gctccccatg aaactccggc
     5941 gcctgctccg tccatatggc ccgcatggca agcacccgct ccctgagcac ggcccaacgc
     6001 tcgtcgaaag cgaccccgtg atgacgcatc tcctcaacgt tccaaccggc accgatgccg
     6061 agcagcaccc ggcccccgga cagacgatcc agagaagcca cctgcttggc gagcacgatg
     6121 ggatcccgtt cggtaaccag cgtgatcccc gtacccacct tcaacgtctt cgtgaccgcc
     6181 gcggccatgg cgagccccac ataggggtcg taggtattcc agtactcgcg cggcagctcc
     6241 ctgcctcccg gccagggcgt ggtccggcag gccggtatgt gggtatgctc cggaaagaag
     6301 atcgactcga aaccgcgggc ctcggccgcg cgcgccaggt ccgaagggga gatggagtag
     6361 gcggtgggga agatgagcac gccgaagtcc gtcatctggc aatctcctta tcaggttgtg
     6421 gtggtgttga gcagatctct gagcgccgac gccagcgccc cgccgggaaa gccgggaggc
     6481 tcggcgaatc gggccgccac gtggccgtcc gggcgcacca gcaccgcgcc acccggctcg
     6541 agcccgaaca gctggagaaa accgccgttg ccggggagca cccgacagtc ggcgttgtgc
     6601 tgagaggccg cttccagcca gcgcgccgag tccgaaagca ccagaaagcg ccgttgcacc
     6661 aggtccaggg tggacagggc gcctccctcc agccacatgt ggggcatgcg gtgaccgggg
     6721 cgggcacagg gcacgtagtc ctccacggca tcctccggct cgggcgccgg gctgccgtcg
     6781 tcgatcaccg ctgccgatcg gtagctgtac cccagctcca gaccccggaa attgccatag
     6841 cgccgcgacc gcgccacctc gcccgccgcg gcctcaccgt cggcgaccgc ccgctggatg
     6901 cgtgccacgg cgcgcgagtt gtccatggcc cgatccgcgt tgaaccgggc caccggcttt
     6961 cgctccgctt cgtaggtgtc gacgagaccg gcgtccgacc agtccctcaa gtcgtaggcc
     7021 agtttccata cgaggttgtg cacgtcctga atgcccgtgt tcaggccgaa tccgccggtg
     7081 ggcggcagct ggtgcgcggc gtctccgacg agaaacgccg cgccctgccg gtatcgttcg
     7141 gccaccgtcg cgttcatggt ccagtgagat atgccaagga tctcgacctt gaggtcgggt
     7201 tcccccgcgg cgttcctaat ccattgtgcg catcgcttgc catcgaaccc ggcgcgagtg
     7261 tccgccgcgc cgtcgtaggc aatctgcgac agccagcgct tgcgcccgtc caggggctga
     7321 aacacgccgc gccccgcttc gttggccgtg aagtaaagca ccgccgggcg atgagccacc
     7381 actcggtcga gattggctcg atagtagacg ttgatgaagt ggccgagatt ctcgggccct
     7441 tccagcccga tgcccagccc ttgccggatc gcaccaccgg ccccgtccgc cgccaggagg
     7501 tagcgggcgc gaacatcgcc ccctctggct ccggtgaggc gcacgacggc tcctgcggcg
     7561 tccacgtcga ggatttccgc ctgatgcccg tagcgcacgt ccaggagcgg ttgctgctcc
     7621 gcccgccctc gccatatggg ctcgaacacg tcctgtgagc tcaaaaccgc cgcgcacggg
     7681 ctggctgccg agcttcggct ctcgaacccc ccggtgccca tgcgtccgag ctcggcaccg
     7741 ctgagcgtat cggcatagac gatctggcgc gtccaggcct cgggaaggcc aacggcctgc
     7801 aactcgggct ccaagccaag ccgacgcatg atctccatgg tccgggagtt gaggttacgc
     7861 gccttgggat gccaggtggt gccggaatgc cgctcgacga gcatcgccgg cacgccgaaa
     7921 taggcgcact cgagcgctgc ggccagcccc acggggccac ctccgacaat gagaacggga
     7981 acttcgagca acgcgcatcc cctggggcgc cagccaagag ggtacgccct tccgggcggg
     8041 ctaggctaga ggcgtcagat caaagaggca ggttgaggag ctgctcgcga tagtggtcga
     8101 tcttggcgac gtagcggtgt ccggccttgt gccgcggggc gtagaggcca acgttgtagg
     8161 catgcagcgc gcacccttcg tcaccgcagc ggtcccgcag atgggaaagc acctgggcac
     8221 cacagtagat gttctccgcg gggtcgtgga ggttggcaat gccgcagaag ttgctccagt
     8281 actccggccg cacctgggcc gggccgatgg cccccacgtg ggagcggacg ttctttcgaa
     8341 acgagctctc ggtcagcacc aggctggcca gcaattcagg ctcgagttcc tgccgtgtcg
     8401 atgcctccag gatccaccct gcaaactccg tggccgtgct cggccgcacg ccaaacgcct
     8461 gggacacctt ggtgccgaac tcgctcacct cggaattcca ggtcacgtag gagttttccg
     8521 gcgctggcac cgcaaacgcg aaagtccggt aggcggcgtc ctcgacgcgg tgcgcaaagc
     8581 ctgcggccag cagcaccggg ccaacgatcg ccaggctgtg cagcacgaag aggaagggcg
     8641 tgtaccagcg cacttttgcg cgcttcgcct ccttccggtt ctcgttctgc cttgccgttg
     8701 ccaccgttgg cacctcgtct cgtgttcata tcgactgtgt acggctgcgc attctaaaga
     8761 cctgaaatgc cacaatgaac ccaaatttcg tgcgaagtcg ttggcaggtt gccaccgaaa
     8821 cgttgggaga atgcaaaaat gcgggccgtg cacggacttt tgtgaggaat tccttaccgt
     8881 ccacgccccg tcacgcttga atcgcgaatc agaacaccgc tcgattactt tttataccta
     8941 aaagtaatca atttttcatt ttttagaaca ttatgccaat ctcagctcgg cgatgatctc
     9001 ctgacggtgt tctttggaaa tgtgcgcata ggcagccgag gtgcgatcga cgatgtcgcc
     9061 gtagcgcgag atgatctgac cggcgatctg ctgcggctcg cccaccacgg cgaacgcctc
     9121 gagcacgtcg tcgctgatga ggtgccccat ctcttcccac cggccctgct tggacatggc
     9181 gttcagctcg ccctgcatct cgccgacgcc gatgctttcc agcaccggct tgtaggcggg
     9241 cgtggaccca tagaaggcga tctgacgcgc cgtggccacc ttcgcttgcg ccagctcttc
     9301 ctcgttctcc ccggtcacga cgaataccgg atagctgatc tcgaagtcgg cgcgcgtctt
     9361 gcccgccttg gcaaaccccc gctccagagc cggcagcgtg gtttcgcgca ggtacttctc
     9421 cgtggtgaac gcgtgaacta tcacgccgtc ggcgacctcg ccggcgacct cggtcatcat
     9481 gggcccgacg gcggcgagaa agacgcgcgg cggtccgtat tcggtgtccg tgggggtgaa
     9541 gaacggcgtc atgagcgtgt gggtgtagaa cttgcccgtg aaggccaggg gctctccctc
     9601 gtgccagttc gaccagatgg ccctcattgc caggatgaat tcgcgcatgc gcgccgccgg
     9661 gtgagacca
//
LOCUS       DBNHKV010000406         2060 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_409753, whole genome shotgun sequence.
ACCESSION   DBNHKV010000406 DBNHKV010000000
VERSION     DBNHKV010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_409753"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..468
                     /locus_tag="AC16PP_13205"
     CDS             10..468
                     /locus_tag="AC16PP_13205"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF017868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HIR6482632.1"
                     /translation="MVARYFAYGSNMNLERVRQRGMRFERAMAGRFHGVRLEFHKTSR
                     VHAGVGHASLAFAPGGVVEGVLYELAGADEIFAMDRFESAPVNYSREIVQIQTEEGAL
                     AGWTYFANPAVIVPGLKPPRSYLEHLLAGRPFLSDPYFRMLESWECADDR"
     gene            450..938
                     /locus_tag="AC16PP_13210"
     CDS             450..938
                     /locus_tag="AC16PP_13210"
                     /inference="COORDINATES: protein motif:HMM:NF016222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HIR6482633.1"
                     /translation="MRGRPVISDRAIAGRINPHIRSRYATLIVGGAREPLYQPAIGRS
                     LAIIRYTRDYAQSVLHEVAHWCLADGERRLLEDYGYWYLPPPRTPEQQAAFFAAEERV
                     QALESLLAAACGLRFHVSADQPGTEVGAFAASVRRRAARWRETGLTGRAAEIHGLLGR
                     PW"
     gene            959..2035
                     /locus_tag="AC16PP_13215"
     CDS             959..2035
                     /locus_tag="AC16PP_13215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6482634.1"
                     /translation="MESVDCVVIGAGVVGLACARRLALDGHEVVVVERRGRIGEETSS
                     RNSEVIHAGIYYPTDSLKAKLCVAGKARLYEYCRERALPHRRCGKIIVASEPAQEAAL
                     QGYAIQAERNGVRLEWLDAAQVNALEPAVRASAGLLSESTGIIDSHAYMVSLQGDLES
                     AGGVVAFNTEVLDLGIERGLRVLTSTVTLECRRLVNAGGLWAPDLARRLDPSAPRAFY
                     ASGHYFTYSGPAPFERLVYPVAEPGGLGVHVTLDLAGQVKFGPDVTWLDGVDYTFDET
                     RLAGFVESIRRYFPAVEPARLQPGYTGIRPKIAGPGEPNADFRIDGPETHGIGGLVNL
                     LGIESPGLTASLAVADLVAERLSA"
BASE COUNT          314 a          654 c          707 g          385 t
ORIGIN      
        1 ggctaggttt tggttgcccg ttactttgcc tacggctcca acatgaacct cgagcgcgtg
       61 cggcagcgtg ggatgagatt cgaacgggcg atggccggcc gcttccacgg ggtgaggctc
      121 gagtttcaca aaacttcgcg cgttcacgcc ggcgtgggtc atgccagcct cgcctttgcg
      181 cccggcggcg tggtggaggg cgtgctctac gagctcgccg gcgccgatga aattttcgcg
      241 atggaccgct tcgagtctgc gccggtgaac tacagcaggg agatcgtgca gattcagacc
      301 gaggagggcg cgctggccgg ctggacctac ttcgcgaacc ccgccgtgat cgttcccggc
      361 ctgaagccgc cgaggagcta cctcgagcac cttcttgccg gtcggccctt cctgtcggac
      421 ccgtactttc gcatgctcga gagctgggaa tgcgcggacg accggtgatc agcgatcgcg
      481 ccattgccgg ccgcatcaac ccccacatcc gctcgcgcta tgcaacgctg atcgtgggcg
      541 gggcaaggga gccgctgtat caaccggcga tcggcaggtc cctcgccatc attcgctaca
      601 cccgcgatta tgcccagagc gtcctgcacg aggtcgctca ctggtgcctg gccgatggcg
      661 agcgccggct tttagaggac tacgggtact ggtatctgcc gccgccccga accccggaac
      721 agcaggcggc gttcttcgcg gctgaagagc gcgttcaggc gctggaatct ctccttgccg
      781 ctgcttgtgg tctgcgcttt cacgtcagcg ccgatcagcc tggcactgag gtgggggctt
      841 tcgccgcatc ggtccgccgc agggccgcgc gctggcgtga gacgggcctc acgggacggg
      901 cggcggaaat ccacgggctt ctcgggcgac cctggtgacg acgagcgggc agggcgcggt
      961 ggagagcgtt gattgcgtcg ttatcggggc gggcgtggtg ggcctggcct gtgcccggcg
     1021 tcttgccctt gacggtcacg aggtggtggt tgtcgagcgt cgcgggcgga tcggcgaaga
     1081 aaccagttcg agaaattccg aggtgattca cgccggcatc tactacccca ccgattcctt
     1141 gaaggcgaag ctctgcgtag ccggtaaagc acggctctac gagtattgcc gggagcgggc
     1201 actgcctcac cgtcgctgcg gcaagatcat cgtcgcctcc gagcctgccc aggaagcggc
     1261 gctgcaggga tacgcgatcc aggcggaaag gaacggtgtg cggctcgagt ggctggacgc
     1321 tgctcaggtg aatgccctcg aaccggcggt gcgggccagc gcggggctgc tttccgagtc
     1381 cacgggcatc atcgactccc atgcctacat ggtgagtctg cagggggacc tggaatcggc
     1441 aggcggcgtt gttgccttca atacggaagt tctcgatctc gggatagagc ggggcctgag
     1501 ggtgctgacg agcaccgtga cgctcgagtg ccgccgcctg gtgaatgcag gtgggctctg
     1561 ggcaccggac ctggcccgtc gtctggatcc ttccgcgccc cgggccttct acgcaagcgg
     1621 ccattatttt acctacagcg gcccagcacc cttcgagcgt ctcgtctacc cggttgcaga
     1681 gccgggcggg ctgggcgtgc acgtcacgct ggatctggcc ggccaggtga agttcgggcc
     1741 cgacgtgacg tggctcgatg gggtggacta caccttcgac gagacgcgcc tggcaggctt
     1801 cgttgaatcc atccgccggt atttcccggc ggttgagccc gcccgcctcc agcccggcta
     1861 caccggaata cgcccgaaga tcgccgggcc cggagagccc aatgccgatt tccgcatcga
     1921 cggtcccgag acccacggca ttggcggcct ggtgaacctt ctcggcatcg agtctccggg
     1981 tctgacggcc tccctggccg tcgccgatct cgtcgcggag aggctgtcgg catgatttgc
     2041 gtggggctgc ttgtcaatcc
//
LOCUS       DBNHKV010000407         3574 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1687053, whole genome shotgun sequence.
ACCESSION   DBNHKV010000407 DBNHKV010000000
VERSION     DBNHKV010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1687053"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="AC16PP_13220"
     CDS             <1..859
                     /locus_tag="AC16PP_13220"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6482635.1"
                     /translation="TVNPRTGYEHRPPPAPASPPKNRLVVGGGPAGMELAAAAAERGH
                     TVTLWERETALGGQMRLAAQVPENASFGEFISFQERRLARLGVTVETGREATAESIAE
                     AGFDVVAIATGATPRGHEASGAHLPFVLEGRDVMSGEAKPGKRVAVIAMEDHLQPLTV
                     ASFLADRGHEVQLIYQTPSIAPLIGKYSIGAPLAKITASGARVRVMERVTSIEQGRIE
                     TANVYSGAGSEITDFDSVVLACGGVADSGLYHSLKGKVDELHVLGDAYAPRRIWFATR
                     QAYALAATL"
     gene            899..1780
                     /locus_tag="AC16PP_13225"
     CDS             899..1780
                     /locus_tag="AC16PP_13225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6482636.1"
                     /translation="MKFGLSMFTTDYSASPTEVAKAAEANGFESFWVSEHSNIPVDTD
                     FPMSDTVPREYSSMLDPFVALAAAATVTERINLGTAICLVTQRDPINCAKLVASIDQL
                     SGGRMLFGIGAGWNEPEMRNHGTDPNTRFRLMRERVEAMKALWTNEVAEYHGRLVNIE
                     PTWQWPKPVQQPHPPVIVAGAGPNVLKRVIAYGEGWLPVVTPVAPEQMKGRVTPMDEF
                     VEMAGELERMADAAGRPTPAITVSGMNLDAATFDAFAELGVERMILRLTTAPLDDVLA
                     QIEQHVETVRSAGGSLH"
     gene            complement(1838..2845)
                     /locus_tag="AC16PP_13230"
     CDS             complement(1838..2845)
                     /locus_tag="AC16PP_13230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018926267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6482637.1"
                     /translation="MMSDTNRQFLLAARPVGMVKESDLQLHEAPVPEPGEGEVLVRVE
                     YISLDPSMRGQMENRADYVAPLALGDVMRARGVGRVVRSNDPEFPVGAVVSGTFGMQE
                     YAIGSDRVDRFRRWDDAVDPKVVLGTLGGTGLTAYFGLLDVGEPKSGDMVVVSGAAGA
                     TGSVAGQIAKIKGCTVIGMAGTDEKCRWLTDELGFDGAINYKTQDVAAELDRLCPDGI
                     DVYFDNVGGEILDICLDRIALNARVVLCGGISRYNVEGELPGPKNYFNLIFRRARMQG
                     FLAPDYAPRFDEAYAELQGWLAEGKIRQRETVIEGFENLPKALVLLFEGANTGKMMVK
                     V"
     gene            complement(2947..>3574)
                     /locus_tag="AC16PP_13235"
     CDS             complement(2947..>3574)
                     /locus_tag="AC16PP_13235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF047238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6482638.1"
                     /translation="WRRQALSEEEVLQSQMLSYPLTQYMFCSPGEGGVALILARADQA
                     HKYTKTPVYLKAAVVRSRRFGSFEVLAPSLALEHNAGPTIDASKAAFEIAGIGPEDVD
                     LAQLQDTESGAEVMHMAENGFCEHGEQEHIIQNGETEIGGRFPVNTDGGCLANGEPVG
                     ASGLRQVYENVLQLRGGAGERQVPGNPKVAYTHVYGAPGISGVNILTT"
BASE COUNT          593 a         1252 c         1167 g          562 t
ORIGIN      
        1 cacggtcaac ccccgcacgg gctacgagca caggccgccg ccggcgccgg cgagcccgcc
       61 taagaaccgg ctggtggtgg gcggggggcc ggccggcatg gagctggcag ccgctgccgc
      121 cgagcgcggt cacaccgtca ccctctggga gcgggagacg gccctcggcg ggcagatgcg
      181 cctggccgct caggtgcccg agaacgcctc gtttggggag ttcatctcgt ttcaggaacg
      241 ccgccttgcc cgcctgggcg tcaccgtgga gaccggccgg gaggccaccg ccgaatccat
      301 cgccgaggca ggcttcgacg tggtggccat cgccacaggc gccacgcccc gcggccatga
      361 agcctcgggc gcccacctgc ccttcgtcct ggagggccgc gacgtgatgt cgggagaggc
      421 caagccgggc aagcgggtgg ccgtcatcgc catggaggac catctgcagc ccctcacggt
      481 tgccagcttc ctggccgatc ggggccacga ggtgcagctc atctatcaga cgccgagcat
      541 cgccccgctc atcggcaagt actccatcgg cgccccgctg gccaagatca ccgcatccgg
      601 cgccagggtg agggtgatgg agcgggtcac cagcatcgag cagggccgga tcgagaccgc
      661 caacgtctac tccggcgccg gctcggagat caccgacttc gactccgtgg tgctcgcctg
      721 cggcggcgtt gcggacagcg ggctgtacca ctcgctcaaa ggcaaggtcg acgagctgca
      781 cgttctcggc gacgcctacg cgccgcgaag gatctggttt gccacgcggc aggcctatgc
      841 cctggcagca accctctgac cgttcggcgg gcacgcttcc gacaaggaga ccaacgccat
      901 gaaattcggc ctgagcatgt tcaccacgga ctacagcgcg agccccaccg aagtcgcaaa
      961 ggccgcggag gcaaacgggt tcgagtcgtt ctgggtgtcc gagcactcca acatccctgt
     1021 ggacacggac tttccgatgt cggataccgt gccccgcgag tactccagca tgctggatcc
     1081 cttcgtcgcc ctggccgccg cggcgacggt caccgagcgc atcaatctcg gcacggccat
     1141 ctgcctggtg actcagcggg atcccatcaa ctgcgcgaag ctcgtggcca gcatcgacca
     1201 gctctccggc gggcgcatgc tgttcggcat cggcgcggga tggaacgagc cggagatgcg
     1261 caaccacggc acggatccaa acacgcgctt ccgcctgatg cgggagcggg tggaggccat
     1321 gaaagccctg tggaccaacg aggtcgccga gtaccacggg cgtctcgtga acatcgagcc
     1381 cacctggcag tggccgaaac ccgtgcagca accccacccg cccgtcatcg ttgccggggc
     1441 agggcccaac gtcttaaagc gggtgatcgc ctacggcgaa ggctggctgc ccgtggtcac
     1501 gcccgtggca cccgagcaga tgaagggccg cgtgacgccc atggacgagt tcgtggagat
     1561 ggccggcgag ctggaacgca tggcggacgc agccgggcgc cccacccccg ccattacggt
     1621 ctcgggcatg aacctggatg ccgccacgtt cgatgcgttc gccgagctgg gagtggagcg
     1681 gatgattctg aggctgacca ccgcccccct ggacgacgtg ctcgcccaga tcgagcagca
     1741 cgtcgagacg gtgcgctccg cgggaggatc actgcactga aacgtcgacg cccgggtgcc
     1801 ggggccggga ggaacgaccg gcgacggctg agcggcgcta gaccttcacc atcatcttgc
     1861 cggtgttggc accttcgaaa agcagcacca gcgctttcgg cagattttca aagccttcga
     1921 tgacagtctc gcgctgcctg atcttgcctt cggcaagcca gccctgcagc tcggcgtagg
     1981 cctcgtcgaa gcgcggggcg taatccgggg ccagaaagcc ctgcatgcgc gcccgccgga
     2041 agatcaggtt gaagtagttc ttcggacctg gcagctctcc ttccacgttg tagcgggaga
     2101 tgccgccgca gagcacgacc cgggcgttga gcgcgatccg gtccaggcag atgtcgagga
     2161 tctcgccgcc cacgttgtcg aagtagacgt cgatgccgtc cgggcacagc cggtcgagct
     2221 ctgcggccac gtcctgagtc ttgtagttga tggcgccgtc gaagccgagc tcgtcggtga
     2281 gccatcggca tttctcgtcc gtgcccgcca tgccgatgac ggtgcagcct ttgatcttcg
     2341 cgatctggcc cgccacgctg ccggtggcgc cggccgcgcc gctgacgacc accatgtctc
     2401 cagacttcgg ctcgcccacg tcaagcaggc cgaagtaggc cgtgagccct gtgccaccga
     2461 gggtccccag caccaccttg gggtccacag cgtcgtccca gcggcggaaa cggtccaccc
     2521 tgtcactgcc gatggcgtac tcctgcatcc cgaacgtgcc gctcaccaca gcgcccaccg
     2581 ggaactccgg atcgttggac cgcaccaccc ggccaacccc ccgggcccgc atgacgtcac
     2641 cgagagcgag cggggcgacg tagtcggcgc ggttctccat ctgcccgcgc atggaggggt
     2701 ccagagagat gtactccacc ctcaccagca cctccccctc gccgggctcc ggaacgggtg
     2761 cctcgtggag ctgcagatcc gactccttga ccatgcccac gggacgggcg gccagcagga
     2821 actgccggtt cgtgtcactc atcagcgccc tcccacggtt tccagggcag acggtgctgc
     2881 cccggatggc ccgtggcgcg ggcgcttaag agcgcgtcgt gcgcgttgag gacagtcatc
     2941 tttcgactac gtcgtgagga tgttcacccc gctgatgccg ggcgcgccgt acacgtgggt
     3001 ataggcgacc ttgggattgc cgggcacctg ccgctcgcct gcaccgccgc gcagctgaag
     3061 aacgttctcg taaacctggc gcagacccga ggcccccacc ggctcgccgt tggccaggca
     3121 gccgccgtcg gtgttcaccg ggaaccgccc gccgatctcc gtttcgccgt tctgaatgat
     3181 gtgctcctgc tcgccatgct cgcagaagcc gttttccgcc atgtgcatga cctcggcgcc
     3241 ggactccgtg tcctgcagct gggccaggtc cacgtcttcc ggaccgatgc cggcgatctc
     3301 gaaggcggct ttggacgcat cgatggtcgg cccggcgttg tgctcgagcg caagggacgg
     3361 cgccagcacc tcgaagctgc cgaaacgccg cgagcgcacc acggccgcct tcaggtagac
     3421 gggcgtcttc gtgtacttgt gagcctgatc cgcacgagcc agtatcaatg ccacgccacc
     3481 ctcgcccggg gagcagaaca tgtactgggt cagcgggtag gagagcatct gcgactgcag
     3541 cacttcctct tcggacagcg cctggcgccg ccac
//
LOCUS       DBNHKV010000408         3962 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1402059, whole genome shotgun sequence.
ACCESSION   DBNHKV010000408 DBNHKV010000000
VERSION     DBNHKV010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1402059"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="AC16PP_13240"
     CDS             <1..471
                     /locus_tag="AC16PP_13240"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6482639.1"
                     /translation="AFLFFTGLTHSGLQFLLRSMGADVVDYDNVQTGGQRTGLYFALL
                     SITAKAGGALAIGITYPLLAVVGFDAQGGNSEDAKFAFRMIYLVVPVMAMILAYVFIR
                     GFKLDEAEQRALQSRIAMRDGSADGEAGAAGSADVDGDGQRAIEGGSDDATRRG"
     gene            449..2131
                     /locus_tag="AC16PP_13245"
     CDS             449..2131
                     /locus_tag="AC16PP_13245"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6482640.1"
                     /translation="MTQLDEADWDLTVDLVVVGSGAAGMACALTAKLEGLDVLMVEKT
                     DRYGGTTALSGGVLWVPNNSLMKQAGVEDSAERAVTYLKHNIGNRVSAARLEAFVENA
                     PKMLDFLRARDCLEVAVFEGFPDYRPEDPGGCQGGRSVEPKVFAGRRLGDALGDLRAR
                     AAMAPAGIVGTMTELRRLAAVRSNPLELLKAWKVFPRNLWNRLSGAHHLANGRALIAW
                     LRHAMERTDVPLWLNAPLARIVTDDDGAVRGVLVRRGEEPVRVEARRGVVITAGGFEH
                     NGEMRSEYFGENTAEYSSGSPGNTGDAIRAGLDAGAAVDLMDDAWWAPTFMPPGQGPQ
                     IVIFERGKPGNIIVNAAGQRFANEADPYNEFVKHMQEADRSGASAIPSFLIFDDRYRS
                     KYPLGGMLPGVTPRRYLENGFVVRADTLVELAEIMGIDPAGLEDTVARFNDMASKGSD
                     DDYHRGDSAFDRFAGDPGVRPNPCLAALDEPPFYGMRIYPGDLGTKGGLLTNEHAQVL
                     RGDGTVVRGLYAAGNSSASAMGNFYPGAGGTIGPAMTFGYIAARHAAAEGSQ"
     gene            2216..3229
                     /locus_tag="AC16PP_13250"
     CDS             2216..3229
                     /locus_tag="AC16PP_13250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6482641.1"
                     /translation="MGGKTINRQLLINGNPRGRAVALSDFVENEAELAPLGEGDVRVK
                     VEYLSFDPSQKGQLENIAGYSRGGAMGEVMYARGIGEVVESRSPGVAEGTKVAGFVGW
                     QEYASLPARSLEIVPDDDLLTARLGPLGGTGLTAYFGLLRVGRPEPGDMVVVSGAAGA
                     VGSMVGQIAEIAGCHTVGIAGGPEKCAWLVDEVGYDAAIDYKNENIKARLKELCPDGI
                     NVFFDNVGGEALNDALARIAPHARVVICGGISRYEQETLPPGPANYFNVVFRQANIEG
                     FLLSGYEAEYDVARERITEWIRSGAIVYKEDVQHGFENIPATLLRLFSGKNFGKQLLR
                     MGD"
     gene            3339..>3962
                     /locus_tag="AC16PP_13255"
     CDS             3339..>3962
                     /locus_tag="AC16PP_13255"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6482642.1"
                     /translation="MSVSLRGKNAIVTGAAVGIGPAYAEALAAEGVNVAVCDIREEIL
                     ELPARLEAHGVRSVGWKADVSVPEDVRKVVDGTVEAFGGIDILISNAGKCWVSVADDD
                     LDKTIADYEGMVGTNLKGEYLMGRAVIAQMLEQGRGGEIVNVATDHMVTCGSPFEICP
                     KLETCPWADSPRPTGGGDVMDVYDASKWALNGFLFAWAKALRPHGIRV"
BASE COUNT          678 a         1202 c         1406 g          676 t
ORIGIN      
        1 gcgtttctgt tcttcacggg tttgacccat tcgggcctgc agtttctgct gcggtccatg
       61 ggggcggacg tggtggacta cgacaacgtg cagacgggcg ggcagcgaac gggcctttac
      121 ttcgcgctgc tctccattac cgccaaggcg ggcggggcgc tcgccatcgg cattacctat
      181 ccgctgcttg cggtggtggg tttcgatgcc cagggtggaa attcggagga cgcgaagttc
      241 gctttccgta tgatctacct cgtggtgccc gtcatggcca tgatcctcgc ctacgtcttc
      301 attcggggat tcaagctcga cgaggcggag cagcgggcgc tgcagtcccg gatcgcgatg
      361 cgtgacggat cggcggacgg ggaagcaggg gcggccggat cggctgacgt cgacggggac
      421 ggccagcggg cgatagaggg aggcagcgat gacgcaactc gacgaggctg actgggacct
      481 caccgtcgat ctcgtggtgg tggggtccgg ggcggcgggg atggcctgtg ccttgacggc
      541 aaagctcgag ggcctggatg ttctgatggt cgagaagacg gaccgttacg gcggcaccac
      601 ggcgctttcc ggtggcgtgc tgtgggtgcc caacaactcg ctgatgaagc aggcgggcgt
      661 cgaggactcg gccgagcgtg ccgtgacgta tctgaagcac aacatcggca accgggtcag
      721 cgcggccagg ctcgaggcgt tcgttgagaa cgcgccgaag atgctcgact ttctccgcgc
      781 gcgcgattgt ctggaagtgg ccgttttcga aggttttccc gattaccggc cggaagatcc
      841 gggcggctgt cagggcggcc ggtcggtaga gccgaaggtc ttcgccggcc gcaggctggg
      901 cgatgcgctg ggcgacctgc gcgcccgcgc cgccatggcg cccgcaggca tcgtcggcac
      961 catgacggag ctgaggcgcc tcgccgccgt gcgctccaac ccgctggagc tgctcaaggc
     1021 ctggaaagtc ttcccgcgga acctgtggaa ccgtctctcc ggagcccatc accttgccaa
     1081 cgggcgcgcc ctcatcgcat ggcttcgcca cgccatggaa cgcacggacg ttccgctgtg
     1141 gctgaacgcg ccgcttgcgc gcatcgtcac cgacgatgac ggtgccgttc gaggcgtgct
     1201 ggtcaggcgt ggcgaagagc ccgtgcgggt ggaagccagg cgcggcgtgg tgatcacggc
     1261 gggagggttc gagcacaacg gcgagatgcg ctcggagtac ttcggcgaga acacggccga
     1321 atactcatcc ggctcgcctg gcaacacggg agacgccatc cgcgcaggtc tcgacgccgg
     1381 cgccgcggta gacctcatgg acgacgcctg gtgggcgccc accttcatgc cgcctggcca
     1441 gggaccgcag atcgtcattt tcgagcgcgg caagccgggt aacatcatcg tgaacgcggc
     1501 gggccagcgc tttgccaacg aggccgatcc ctacaacgag ttcgtcaagc acatgcagga
     1561 ggcggatcgc agcggggctt ccgccatccc gtcgtttctc atcttcgacg accgttaccg
     1621 gagcaagtat cccctgggcg gcatgctccc tggcgtcacg cctcggcgat atctggaaaa
     1681 cgggttcgtc gtgcgggcgg acaccctggt cgagctggcc gaaatcatgg ggatcgaccc
     1741 ggccggtctc gaagatacgg tggccaggtt caacgacatg gcctcgaagg gcagcgacga
     1801 cgactatcac cggggcgata gcgcgttcga ccgcttcgcc ggtgatcccg gggtacgacc
     1861 caacccctgc ctggctgccc tggacgagcc gcccttctac ggcatgcgga tttacccggg
     1921 cgatctcggt accaaaggcg ggttgctcac caacgagcac gctcaggttc tgcgtggcga
     1981 cggcaccgtg gttcgcggcc tgtatgccgc ggggaacagc tcggcctcgg ccatgggtaa
     2041 cttctacccc ggtgccggcg ggaccatcgg gccggcgatg acctttggtt acatcgccgc
     2101 ccggcacgcc gccgccgagg gtagccagta ggcggttaaa gtaccatcgt gacggcgcgg
     2161 gggcgctgcg ggtggggccc gtcgcgcaga cggatttgat gtcaggggaa agcaaatggg
     2221 tgggaagacc atcaacagac agttgctcat caacggaaac ccgcgcggca gagccgtggc
     2281 gctgagcgac ttcgtggaga acgaggcgga gcttgcgccc ctgggagaag gcgacgtccg
     2341 ggtaaaggtg gagtacctga gcttcgaccc gtcccagaag gggcagctgg agaacatcgc
     2401 cggctattcc cggggaggcg ccatgggcga ggtgatgtat gccaggggca ttggcgaggt
     2461 ggtggagtcg cgcagcccgg gcgtggcgga gggcacgaag gttgccgggt tcgtaggctg
     2521 gcaggagtac gcgtcgctgc cggctcgcag cctggagatc gtgcccgacg atgatctgct
     2581 caccgcccgg ctcggtccgc tgggcggcac cggcctcacc gcctacttcg ggctgcttcg
     2641 ggtgggccgg ccggaacccg gcgacatggt ggtggtgtcg ggtgcggccg gggccgtggg
     2701 ttcgatggtc ggtcagatag ccgagattgc cggctgccac accgttggga tcgcgggcgg
     2761 cccggaaaag tgcgcatggc tcgtggacga ggtcggctac gacgccgcca tcgactacaa
     2821 gaacgagaac atcaaagccc gcctgaagga gctgtgcccg gacgggatca acgttttctt
     2881 cgacaacgtc ggcggggagg cgctcaacga tgcgctggcg cggatcgcgc cccacgccag
     2941 agtcgtcatc tgcggcggca tatcccggta cgagcaggaa accctgccgc cgggtcctgc
     3001 caactatttc aacgtcgtgt tcaggcaggc caacatcgaa ggctttctgc tgagcggcta
     3061 cgaagccgag tacgacgtcg ccagggaacg catcaccgaa tggatccgtt ccggggccat
     3121 cgtatacaag gaagacgtcc agcacggctt cgagaacatt ccggcgacgc tgctgcggct
     3181 gttctccggc aagaacttcg gcaagcagct gctcaggatg ggcgactgag gtgccgtctc
     3241 cggccggatc gatccccggt tggggcacgc tgacgcagac tgaagccttc gtgtattctc
     3301 gtggcgggag ttcaatcaca tctcgatgag gggggatgat gtcagtttca ctgcgtggca
     3361 agaacgccat cgttacgggc gccgccgtcg gcattggtcc ggcctatgcg gaagcgcttg
     3421 ccgctgaagg ggtcaacgtc gccgtttgcg acattcgtga agagattctc gagttgcccg
     3481 cccgcctgga ggcccatggc gtccggtccg taggctggaa ggccgacgtg tccgtgcccg
     3541 aggacgtgcg caaggtggtg gacgggacgg tcgaagcctt cggcggcatc gacatcctga
     3601 tcagcaacgc cggcaagtgc tgggtcagcg tggccgacga cgacctggac aagacgatcg
     3661 ccgactacga ggggatggtg gggaccaacc tcaaaggcga atacctcatg ggcagagcgg
     3721 tgatcgccca gatgctcgag cagggccgtg ggggcgagat cgtcaacgtg gccaccgacc
     3781 acatggtcac ctgcggctcg cctttcgaga tctgcccgaa gctcgaaacc tgtccctggg
     3841 cggattctcc ccggcccacg ggcgggggtg atgtgatgga cgtctacgat gcgtccaagt
     3901 gggcgctgaa cggatttcta ttcgcctggg cgaaagcgct tcgcccccac ggtatccgcg
     3961 tc
//
LOCUS       DBNHKV010000409         3941 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1687233, whole genome shotgun sequence.
ACCESSION   DBNHKV010000409 DBNHKV010000000
VERSION     DBNHKV010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1687233"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="AC16PP_13260"
     CDS             <1..861
                     /locus_tag="AC16PP_13260"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR6482643.1"
                     /translation="MVASGMENVVMCCGVEMMTRVPLGSNMKDGVPMGASYMEHYQPT
                     SQFQGAAMIAEEYQLTREDTDAFGLKSQKNAARAWQEGRFDREIAPVEAPVLDESGQP
                     TGETRVVERDEGPRETTLEGLQKLEPVPGQPIHTAGTSSQVSDGAAVVILANRKAVDE
                     LGLTPRARIIATTLVGVDPVTMLKGPIPASRKVLDQAGLGVDDIDVFEVNEAFASVVL
                     AWQKELNPDPERVNPNGGAIALGHPTGSTGARLITTALHELERSGGRYGLIAMCCGGG
                     LGTGTIIERI"
     gene            complement(865..1593)
                     /locus_tag="AC16PP_13265"
     CDS             complement(865..1593)
                     /locus_tag="AC16PP_13265"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6482644.1"
                     /translation="MFEEPNRRRIYLLRHAEAAYLDDDGNITDDVRTVGLTPTGRIQA
                     RKQAAVLASVEFDRAVCSGLPRTVETAKHILAYRDHPELETVSALEEIQGARGLAPQA
                     DVKAWLAMVANPWSGADHPEAQFLGGERFVDFAARVTPAFDAIVADHSWQTLLLVLHG
                     AVNRMLFNHIIGIPWSAKTSIEQDNCCINVIDVDTAEDGATERFLIRGVNLTAYDLNK
                     SSFYLTHMEQAAQRMAGRVTGNAL"
     gene            complement(1586..2416)
                     /locus_tag="AC16PP_13270"
     CDS             complement(1586..2416)
                     /locus_tag="AC16PP_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6482645.1"
                     /translation="MTYTRMNVSAENGVGRIELNQPDEYNRMPLAFWRELPLALRKLD
                     AGGDVRALVLSSTGKHFSAGMDLSVFTGSARRDLDRGRAGEAGRRHIDRMQNVFNGLE
                     AVRMPVLAAVQGGCVGGGVDLVAACDARYCTADAFFCIQEINIGMAADVGTLQRLPRL
                     IPDGLMRELAYTGRRMHADEALSCGLVNAVYDSHEQMLEAVGAIAAEIASKSPLAVTS
                     TKHLLNYSRDHGVHDALAYQQLWMGAVTQGGEMAEYFKAKADGRDPVYADLPPLEDDV
                     "
     gene            2732..>3941
                     /locus_tag="AC16PP_13275"
     CDS             2732..>3941
                     /locus_tag="AC16PP_13275"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6482646.1"
                     /translation="MLTFADPLRRAVQIAAEDTAVIHDGARYSYAEFNRRCGQLVAVL
                     RELGIQRGDRVAILAGNCNQYLETYVGVPAGGMVVVPLNTRHAQPELEYALKDSGARV
                     LLTDRDPGALGGIVDHVISIPDAYESHLAHAEPAGLGEGVSEADLAGLFYTGGTTGLS
                     KGVMLTHRNLIANTFSWLACSPHYSSDRTLIMAPLFHAAGSNGVLGAIWNGGCQVTLG
                     AFDPASALDLIEAHAITETLGVPTMLAAIAEEQHARPRKTDSLRLIAHGGSPIATEVI
                     RRTMSAFPTASMMEVYGATELAPLATVLVGEEKLVDDLRARSCGQALIGNEIRILNPD
                     GQTVDTGEVGEVVVRGPNVMEGYWNKAEQTAAVLKDGCYWTGDLGYMDDRGYLFLVDR
                     SKDMIVSGGEN"
BASE COUNT          681 a         1225 c         1350 g          685 t
ORIGIN      
        1 atggtggcct ccggcatgga gaacgtggtc atgtgctgcg gcgtggaaat gatgactcgg
       61 gtcccgctgg gatcgaacat gaaggacggc gtgcccatgg gggcgagcta catggagcac
      121 taccagccaa ccagccagtt ccagggcgca gccatgattg ccgaggagta ccagctcacc
      181 cgtgaagata cggacgcatt cgggctgaag agccagaaga acgcggcccg tgcctggcag
      241 gaagggcgct tcgaccgcga gatcgcaccc gtcgaagcac ccgtgctcga cgagtcgggc
      301 cagcccacgg gagagacccg ggtggtcgag cgcgatgagg gtccccgcga gacgacgctt
      361 gaaggattgc agaaactgga gccggtgccc ggccagccga ttcacacggc cgggaccagc
      421 tcccaggtct ccgacggcgc ggccgtggtg attctcgcca accgcaaggc ggtggacgag
      481 ctggggctca ccccgcgcgc ccggatcatt gccaccacct tagtcggggt cgatccggtg
      541 accatgctca agggtccgat ccccgcatcg cgtaaggtgc tggatcaggc gggtctgggc
      601 gtggacgaca ttgacgtgtt cgaggtcaac gaagcatttg cttcggtggt gctggcctgg
      661 cagaaggagc tcaatccgga tcccgagcgc gtgaacccca atggcggggc cattgcatta
      721 ggccatccca ccggctcgac cggggcgcgc ctgatcacca ccgcgctgca cgagctcgag
      781 cgcagcggag gtcgctacgg gctgatcgcc atgtgctgcg gcggcgggct gggcacggga
      841 accatcatcg agcggatctg aatctcaaag cgcgttgccg gtcacccggc cggccatgcg
      901 ctgggccgcc tgctccatgt gggtcaggta gaagctcgac ttgttgaggt cgtaggccgt
      961 gaggttcacg ccgcggatga gaaaccgctc ggtggcgccg tcctccgcgg tgtccacgtc
     1021 gatcacgttg atgcagcagt tgtcctgctc gatggacgtc ttcgcgctcc acggaatgcc
     1081 gatgatgtgg ttgaagagca tgcgattgac cgcgccgtgg agcacgagca gcagggtctg
     1141 ccaggagtgg tcggctacga tggcatcgaa agcgggggtg acccgggcgg cgaagtcgac
     1201 gaagcgctcc ccgccgagga actgcgcttc cggatgatcg gcgccggacc agggattggc
     1261 gaccattgcg agccacgcct tcacgtccgc ctggggcgcc agaccgcggg cgccctggat
     1321 ttcctctaga gcagagaccg tctccagctc tggatgatcc cggtacgcga ggatgtgctt
     1381 tgccgtctcc accgtgcggg gcagcccgga acacaccgcc cggtcgaact ccacgctcgc
     1441 aaggacggcc gcctgcttgc gtgcctgtat cctgccggtg ggtgtcaggc cgacggtgcg
     1501 gacgtcgtcg gtgatgttgc cgtcgtcgtc gaggtaggcc gcttccgcgt ggcggagcag
     1561 gtagattcgg cggcggttcg gctcctcaaa catcgtcttc cagcggcggc agatcggcat
     1621 aaaccggatc gcgcccgtcg gccttggcct tgaagtactc ggccatctcg ccgccctggg
     1681 tcacggcgcc catccacagc tgctggtagg ccagcgcgtc atgcacgccg tggtcgcgcg
     1741 agtagttgag caggtgcttg gtgctggtga ccgccagcgg agatttgctg gcgatctccg
     1801 ccgcgatggc acctacggct tcgagcatct gctcgtggga atcgtaaacg gcgttgacga
     1861 gcccgcagga aagcgcttcg tccgcatgca tccgccggcc cgtgtaggcg agctcccgca
     1921 tgaggccatc cgggatgagt cgggggagcc gctgcagggt gccaacgtcc gcggccatgc
     1981 cgatgttgat ctcctgtatg cagaaaaagg cgtccgccgt gcagtagcgg gcatcgcagg
     2041 cggccaccag atccacgccg cccccaacgc agccgccctg aacggcagcc agaacgggca
     2101 tgcgaaccgc ctccagaccg ttgaatacgt tctgcatccg gtcgatgtgg cgccgtcccg
     2161 cctcgccggc gcgtccacgg tccaggtccc gtcgggcaga gcctgtgaag acggagagat
     2221 ccatgccggc actgaaatgc ttgccagtgg aggagagaac gagcgcccga acgtcgcctc
     2281 cggcatcgag tttgcgcagc gcgagaggca gctctctcca gaaggccagc ggcatgcggt
     2341 tgtactcgtc cggctggttg agctcgattc ggccgacccc gttttcggcc gagacgttca
     2401 ttcgagtgta cgtcatgaca gtctcgttgc ggttaaccaa gcatgttacc gattctgccc
     2461 gcgggcggac accgtcggct gttggaagat ggcattgcca gcgccctgga agccgctgat
     2521 aatcggcccc gtgtcgggca acagataagg aggtgatcag tgtatctatc tgcaacctat
     2581 gcagaggagg ggctggtctc ctgatcggct tctcgagaac gggcccctca acggggcccg
     2641 ttttttttgg cctgccaggt cgggcgtatt tccacggccg gacctttctg ggagactccg
     2701 gcgatctgcg ggaaatgttt ccgggggaaa gatgctgaca ttcgcggatc cgttgcgtag
     2761 ggccgtccag atcgccgccg aggacacggc ggtgatacac gatggcgcgc gctacagcta
     2821 cgcagagttc aatcgccgct gtggccagct cgttgctgtt ctgcgcgagc tcggaattca
     2881 gcgcggtgac cgggtggcga tcctggcggg caactgcaac cagtatctcg aaacctacgt
     2941 cggggtcccc gcgggcggca tggttgtggt gccgctgaat acccgtcatg cccagccgga
     3001 gctcgaatac gcgctcaaag attccggcgc ccgcgtgctg ctgaccgacc gcgatccggg
     3061 ggcgctcggc ggcatcgtcg accatgtgat cagcatcccc gatgcttacg agtcgcatct
     3121 cgcccatgcc gagccggccg ggctcggaga gggcgtgagc gaggcggatc tggccggtct
     3181 gttttacacc ggaggcacga cgggactgtc caagggggtg atgctcaccc atcggaacct
     3241 catcgccaac acgttcagct ggctggcctg ctcgccgcat tactcctcgg atcgcaccct
     3301 catcatggcg ccgctgtttc acgcggcggg gtccaacggc gttctcgggg caatctggaa
     3361 cgggggatgc caggttacgc tgggtgcctt cgatccggcg tcggcgctgg atctgataga
     3421 agcccatgcc atcacggaaa ccctcggggt gccgaccatg ctggccgcca tcgccgagga
     3481 gcagcatgct cgtccccgca aaacggactc cctgaggctg atcgcccacg gcggttcgcc
     3541 catcgccacc gaggtgatcc ggcgcaccat gagcgcgttc cctacggcgt ccatgatgga
     3601 ggtctacggc gccacggagc tcgctccgct ggcgacggtg ctggtgggag aggagaagct
     3661 ggtcgatgat ctccgggcac gctcgtgcgg gcaggccctg atcggcaacg agatccgtat
     3721 tctcaacccc gatgggcaga cggtggacac aggggaggtc ggcgaggtcg tcgttcgcgg
     3781 gcccaacgtc atggaaggtt actggaacaa ggccgagcag acggcggcgg tgctcaagga
     3841 cggctgttac tggacgggcg acctcggcta catggacgac cggggctatc tgtttctggt
     3901 ggaccgcagc aaggacatga tcgtctcagg gggcgagaac g
//
LOCUS       DBNHKV010000410        14640 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_270419, whole genome shotgun sequence.
ACCESSION   DBNHKV010000410 DBNHKV010000000
VERSION     DBNHKV010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 14640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14640
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_270419"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="AC16PP_13280"
     CDS             <1..320
                     /locus_tag="AC16PP_13280"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6482647.1"
                     /translation="SISAQEGKELGMVNRVVKREDLMSETLALAEKIARQPSMGLKLS
                     KLAVNQSLDAQGMWTAIQSAFNLHHLGHAHMRIMHGQAVEPAGAKKIKTQARADKPKL
                     TGP"
     gene            complement(335..1366)
                     /locus_tag="AC16PP_13285"
     CDS             complement(335..1366)
                     /locus_tag="AC16PP_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HIR6482648.1"
                     /translation="MKRIVICADGTWNRPERNLAEDQPTNVLRLARAIRPSGSTGTAQ
                     QVFYDWGIGSYYDPFVGGVTGRGINKNIMDGYRYLVQNYAPGDQIFLFGFSRGAYTVR
                     SLCGLINNCGILKRPDARLIQAAFDHYKRPGSAHHPDGPRSREFRAEHAHPSRRIRFV
                     GVWDTVGALGIPFSFLGMLDREDEFYDTKIGPNIEIARHAMAIDERRSDFEPTIWQQR
                     TGTDISQVWFAGAHGNVGGGLYREGKGGRLSDIALAWMINEARGAGLSVEGHLGDRLR
                     PDPLAPVYGSRRHIYRSRPRADRPIDHGSAPVLVHESVRQRWDALPGYRPRNLVRYLE
                     INGWPTLVP"
     gene            1428..2318
                     /locus_tag="AC16PP_13290"
     CDS             1428..2318
                     /locus_tag="AC16PP_13290"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6482649.1"
                     /translation="MQRKDHIDTLAAVTLIVMNVLLGLNQSVVKLVNDGFAPVFQSGL
                     RSACAILPVLGYALVMRKRLSVTDGSLGLGMLNGLLFSGEFCLLFLALDYTSVARVSL
                     FFYVMPVWVTIGAHFLVPGERMTLLKAGGLVLALAGVALGLGIGSGSAGEQAWIGDLL
                     ALGGAIFWASIALLTRTTRLQRCSPEMNLLYQLAVSAVILLAVAPLFGDTVREPTPAI
                     LGMFAFQVIVIVAIGFVVWFWILSIYPVSHMASFSLLAPVFGVLFGWLIFDDQITPAF
                     VIALLLVGSGIVLINRSPAG"
     gene            2323..3333
                     /locus_tag="AC16PP_13295"
     CDS             2323..3333
                     /locus_tag="AC16PP_13295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6482650.1"
                     /translation="MDNGRWTVASRPEDQVEEANFQFVSESVGEPEDGEFLIGTRYLV
                     VSPPARMALTSGGIARRPVPIGATMRGTGLGQVVASRHPGFAEGELVIGDLGWQEYAI
                     SDGATRHPVRKVAARPGMPESTLLHVLGAGGATAYFGMIEYAKPHVGDTLVVSAAAGN
                     VGSLVCQLGRLQGCRVVGIAGSDEKCSWLTNDLGCAAAINYKREDVAAALDGACPDGI
                     DVYFDNVGGEILDAALARIAQGARVVLCGGTSQYNHDLDWYGPKNYFNLVYRQAEMAG
                     FYVYNFSHRFEEAFGRLESLLATGELKYAEDVLQGIGQTPAALIRVLKGENFGVQLVR
                     VA"
     gene            complement(3320..6541)
                     /locus_tag="AC16PP_13300"
     CDS             complement(3320..6541)
                     /locus_tag="AC16PP_13300"
                     /inference="COORDINATES: protein motif:HMM:NF017021.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR6482651.1"
                     /translation="MGWLRATLIAVLAVVVALGAMAVWLTQADLSFLKPQVERLIAEQ
                     TGRAFTIGGEFSLNAGRRTAIVAEDVRLGNAEWASDPEMARIGRLRLELDLLSLLRGP
                     ARIHLIELEDASVLLENPADRDGNWVISADEPDAADSLTLPVLGDIVVDALSVTYLDP
                     ELEGPLSIEVVDARQHFDPETDGLSGSLQATVNGRSVVVSSHVSPLPAVIAGEDFDFE
                     LSLIAKALTLTASGHMDDLGRPRRPALKVNLMSPDVDLITDALGAEEFGDGGLDLNIE
                     LTPGTDTVALTATGDLAGLRVRVDAAADDLSSMRESSLNLSVQGPRFGRVMDVFGLEG
                     LPDDPFQASGQVKRSGPVLTLSDVLLEVEGTRLEIEGQTRGFPGIDAASLRLRLEGRE
                     LGRFQRFLSLPDWAVGPFTVAGSIEHSEGQTDTLDLAAATSLANVSLRGPIGDPPEYG
                     GTELSLTASGRDLRALVKVTESLEPTALEAVPAKPFSSGGRLTVKRRVFELSAVYLEI
                     EESRFDLEGSLSRRADLVGSRVTVTGRGGELESLIADAAGLDFKPAPFEVTATLERTP
                     ETLQIQDLLLTNERGGEVRLNAELGLPFPASRAADVNLVARGPDVSALPVQPQRLELE
                     PLPFEALLRASLRGDRLTVDKGSLVLGEAELDWRGTFDLPPEFSATESTLSVNVPELA
                     ALGRLDGEPLPAAPLSLSARASGTSTTLRLEGIEAELGASALSGDLQLDVGGTRPRLQ
                     AKIHIPTLDVPVSEDAQEARRPPPADGRLIPDVALPVDGLDVMDGALELTVDELRFGD
                     LTFTQGFLSAVLEDGNLLVERLDIRPPSGRVQGRLAVRPEDESGTIDLELRGDRVALN
                     VAANLEPSAEPADQLVFDVDVYLRARGTDARALAASLEGEVSALSDGGRIHNRQLALI
                     FGGFGRELLGNINPFIKHDPYTDLSCVVFRFSATDGILKADPYLAIRSDKLNLVSSGF
                     ANLATERIDFTFNALPRLRLSISATELINPYVRVSGTLLKPTLSLDSRSAAVTGGAAA
                     LTGGLSLLAQATWKRTFGSRDACGKALEELRAEEGAAAAVTPP"
     gene            6664..7284
                     /locus_tag="AC16PP_13305"
     CDS             6664..7284
                     /locus_tag="AC16PP_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_259446992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:6706..6708,aa:Sec)
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HIR6482652.1"
                     /translation="MTDQLKLEIFSDYIUPWCYLSTGRIEKLKSSFDLEVRWVAFPLH
                     PETPEEGITIEALFAGREIDLDAMYVRMKALMDEEGLPYGRRTHTFNSRLAQELGKWA
                     DSRPGGEAIHDALFRAYFVDGLNIGDAGVLVDIAKSAGLDPGGAREVIDQRTFSNAVD
                     EDWKRSRRYGITGVPTFVAGGYGVVGAQPYETLEHLVQRALTESRA"
     gene            complement(7303..7896)
                     /locus_tag="AC16PP_13310"
     CDS             complement(7303..7896)
                     /locus_tag="AC16PP_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6482653.1"
                     /translation="MPDPEQPGELPFRPADAILRPESFLPAADGTEVCAFLNPYDARG
                     AASAHSAGEAISMAAGRIGPGVRSAIHVHPALTQITYVTAGTLTARMRDPGESEPSRL
                     EVPTGAAVLTEAGTLLQLCNETDRTVEVLYVSTPAYVLETAADGAIRYEDALILGADW
                     DAPAVAHWNVETATQARSRATARRAEAAGRIAGRTRR"
     gene            complement(7889..8134)
                     /locus_tag="AC16PP_13315"
     CDS             complement(7889..8134)
                     /locus_tag="AC16PP_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482654.1"
                     /translation="MSLIRYPVVQYAYVVDDIEAAGYPAVTTLTSAARVAYMDTRPIL
                     GCFVELYEDNAPLRETFAHWKAVHDAWNGVSDPIRDA"
     gene            8265..9311
                     /locus_tag="AC16PP_13320"
     CDS             8265..9311
                     /locus_tag="AC16PP_13320"
                     /inference="COORDINATES: protein motif:HMM:NF053113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-dihydroxypyridine 5,6-dioxygenase NicX
                     family protein"
                     /protein_id="HIR6482655.1"
                     /translation="MLQERIEGKWIDCFARVFERCAVAENEEVAILSESQSRPANAQL
                     AELALLRLGARPYHLVIPTPPQKAPVPVRSTGATDVIGHSRSVVAALSSAGFVVDCTV
                     EGMLHAPELPEILKGGARVLMISNEHPEALERLEPDEALETRVKAGIRKLRGASQMKV
                     TSAAGTDLTVSLEGSVAGGGWGYVARPGIMSHWPGGLCLCFPAAGSVSGVLVLDRGDV
                     NLTFKRYLENPVALSIDADYVTEIDGAGVDAELMRSYFAAWGDQEAYAASHLGWGMNN
                     AARWDAMTMYDRNDFNGTELRAFGGNFLYSTGANEVAGRHTLGHFDLPLRNCSVHLDG
                     EAVVVDGALQRELA"
     gene            9308..9883
                     /locus_tag="AC16PP_13325"
     CDS             9308..9883
                     /locus_tag="AC16PP_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6482656.1"
                     /translation="MSVPGFKEAGSGEPVVFLHGIGGNRHAFDAQLAEPGRVRSLVLA
                     QTMARFGKPGSDWQQQFLDARLKPLEDGRAPADFARELITGMFGDPSRAEAIERAVAT
                     MSPLPADVYRQVLTCLVVFDQRGTLPEIDAPTLCLAAELDQTAPPRTMEKMATEIPAG
                     RYDCLQDAGHLAYVERPAAFTRAVNEFLGQL"
     gene            9883..11073
                     /locus_tag="AC16PP_13330"
     CDS             9883..11073
                     /locus_tag="AC16PP_13330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6482657.1"
                     /translation="MTKTEELIGIARELGRDRFLPRRRTYDEEARFPDENYEDLREAG
                     FLGLAIDRRYGGLGADFGTYASISVELGRWCGATALTFNMHTSTMLWSGQLADDLPMS
                     DAERSAHESRRAEIYAGVIEEGHIFAQPFSEPNSAAAAGKAAFDTSAVKVDGGWLVNG
                     RKHFASLSGSAHFYGVLCTDLTDGREPDVRNTLLLAVPASASGFSVAGDWDVLGMRGT
                     VSRSLVLEDVFVPDSGQLLPQGCYYQAALGWPHMFMTLCPTYLGIAEAAFDFTCQYLR
                     GEVEGGPPAGGARDNPVKQVGVAEMRIKLEQARALFEKAIGEAAYRPSKDERLRAYAA
                     QYTVMEFANDICRQAIRVCGGRSLFKSLRLEQLYRDSRCGSLMLPWTPDICIERLGRE
                     SLES"
     gene            11078..12589
                     /locus_tag="AC16PP_13335"
     CDS             11078..12589
                     /locus_tag="AC16PP_13335"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0032787 - monocarboxylic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6482658.1"
                     /translation="MNGLGDWIRCNADWAGGRPALRLGETAYTYAELDAAVDRMAFLL
                     RDGLSIRRGDVVAFLGLNSEQMIVLLFACARNAASLLPLNNRLAGPEHEWILRHSGAR
                     FLFVEDEFAAQANAIDVPGLERVAMGAPPEPHWHEFQRMLGDAREGGFEDVGTLDDRV
                     LLIYTSGTTGQPKGVAHDQRALFYNALNAIHAQEMRASDHVLTVLPLFHAGGLNIQTT
                     PAFYIGAGVSLMRRFDPGEALRAIETLKPTMFLAVPAVITAMVDHPAWSETDLSSLRM
                     VGTGSSTVPDPLLDAWHDRGVPATQIYGLTESAPTAICLPIAETRRKRGAAGKPVIHC
                     EARIVDDGGNILGPGERGEIVLKGPNLFCEYLNDPETTAAAFSDGWFHTGDIGHMDAE
                     GFFYVDDRKKDVVISGGENVYPAELENVLAEMPELAEYAVVGRPDERWGEVPVACVVL
                     RDGADLGEDELIARFAGRLARYKHPREVRFFQSLPRNVMGKVLKFRLREEVAP"
     gene            complement(12594..14120)
                     /locus_tag="AC16PP_13340"
     CDS             complement(12594..14120)
                     /locus_tag="AC16PP_13340"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6482659.1"
                     /translation="MNVLASQLEPWAVADRQPRIAIIGAGMSGIAAVVKLRKAGYTHL
                     TVYEKADRVGGTWRENTYPGLSCDVPSRWYSFSFALSSEWTHRFSYGPEIQAYMEKVA
                     DGFGVTDVVRFDTPVTELTYEAPFWRLTTAAGEQQIYDVVISATGILHHPAMPDIEGL
                     EDFAGASFHSARWDHSVDLTGKRVGVIGTGSTAAQIVGDIAGSVGELHVFQRTPQWMV
                     PMPQKTYSARWKRVLKLFPFLKRLAYQYYFRLMVRTFAMATVGDEKMQARISKITRQN
                     LERNVKDPELRRRLTPDYQAACKRLILCSDFYPAITRDNAHLVTDAIERVTPAGIRTA
                     DGRVHELDVLVLATGFDPSAFILPTKVTGEDGVDLESFWQGAPRALRAVAIPRFPNFW
                     MLEGPTGPVGNMSLITITEHQVDYVIAMLERMKKRRLVAVSAREDAYRNYNQAMQEAV
                     KNTVWMTGGCTSWYIDKTGIPNLYPWEPMRYLKSMRNPDFSEYREETGQGKEAALAAA
                     "
     gene            14245..>14640
                     /locus_tag="AC16PP_13345"
     CDS             14245..>14640
                     /locus_tag="AC16PP_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6482660.1"
                     /translation="MNVKSTRVRRNPEAARAEILAAAMAALQDVRFSELTVDSLMKRT
                     GMTRSSFYHYFSGLDEIALGMLEEFAADIRRTVDPWLTGELDEPDHRAATLTHLTSMF
                     EVFERHAPSVAAVAQAAGGSQEVYQEWQRG"
BASE COUNT         2375 a         4727 c         4937 g         2601 t
ORIGIN      
        1 agtcgatttc cgcccaggag ggtaaggaac tcggcatggt caaccgcgtg gtcaagcggg
       61 aagacctgat gagcgagacc ctggcccttg cagaaaaaat cgctcggcag cccagcatgg
      121 ggctgaagct gtccaagctc gccgtcaacc agtccctgga tgctcagggc atgtggacgg
      181 ccattcaatc ggcgttcaac ctgcatcacc tcggccacgc ccacatgcgc atcatgcacg
      241 gccaggccgt ggagcccgcc ggcgctaaga agatcaagac tcaggcccgg gcggacaagc
      301 ccaagctcac tggcccttga agcgagccgc cggctcaggg aaccagcgtc ggccagccgt
      361 tgatctcaag gtagcggacc aggttgcgcg gccggtagcc tggcagcgca tcccagcgct
      421 ggcgaacgct ttcgtgaacg agcaccggag cgctgccgtg gtcgatggga cgatccgctc
      481 ggggccggga gcggtagatg tggcggcgtg acccgtacac gggcgcgagg ggatcgggcc
      541 gcagccggtc acccaggtgg ccctccactg aaagcccggc gccccgggct tcgttgatca
      601 tccatgccag cgcgatgtcc gaaagccggc cacccttgcc ttcccggtag agcccgcccc
      661 cgacgtttcc gtgggcgcct gcaaaccaga cttgtgagat gtcggtgccc gtgcgctgct
      721 gccagatcgt aggttcgaag tcggagcggc gctcgtcgat ggccatggcg tggcgggcga
      781 tctcgatgtt gggaccgatc ttggtgtcgt agaactcatc ctcacgatcg agcatcccca
      841 ggaaggagaa cggaatgccc agcgcaccga cggtgtccca gacgcctacg aagcggatcc
      901 tgcggctggg gtgcgcatgc tccgctcgaa actcccggga gcgcgggccg tcgggatgat
      961 gggcgctgcc tggtcgtttg taatgatcga atgccgcctg gatgagcctg gcgtcagggc
     1021 gcttgaggat gccgcagttg ttgatcagcc cgcacaggct gcggaccgtg taggcgccgc
     1081 ggctgaagcc gaacaggaag atctggtcgc cgggggcgta gttctgcacc aggtagcggt
     1141 atccgtccat gatgttcttg ttgattcccc gtcccgtcac gccgccgacg aagggatcgt
     1201 agtaagagcc gatcccccag tcgtaaaaca cctgctgggc ggtgcccgtg ctgcccgatg
     1261 gccggatggc cctggccagc cgcaggacgt tggtgggctg atcttcggcc aggttgcgtt
     1321 ccggtcggtt ccatgtgcca tccgcacaga tgacgatcct cttcacttgc tccctccccg
     1381 ggtttcgttg atacccgttc ggttcactgc ggctatcatg ctgccccatg cagcgcaaag
     1441 accacataga cactctggcg gccgtaacgc tcatcgtcat gaacgtcctg ctgggactga
     1501 accagtccgt ggtcaagctt gtcaacgatg gatttgctcc cgtgtttcag tcgggattgc
     1561 gctccgcgtg tgccatcctg cccgttctcg gctacgcgct cgtgatgcgc aagcgtctct
     1621 cggtcaccga tggcagcctg ggcctgggga tgctcaacgg cctgcttttc tccggcgagt
     1681 tctgcctgct cttcctcgct ttggactaca ccagcgtggc cagggtctcc ctgtttttct
     1741 acgtcatgcc ggtatgggtg accatcggcg cccactttct cgttccgggc gagcggatga
     1801 cgctgctcaa ggccggcggc cttgtcctcg cgctggccgg cgtagccctg ggtcttggca
     1861 tcggctccgg ctcggccggc gagcaggcct ggatcggaga cctgctggcg ctgggaggcg
     1921 cgatcttctg ggcgagcata gcgctgctca cccgtaccac ccggctgcag cgatgctcgc
     1981 cggagatgaa cctgctctac cagctcgccg tatccgccgt gattctgctg gcggtggcgc
     2041 ctctgttcgg cgacacggtg cgtgagccca caccggccat cctcgggatg tttgcgtttc
     2101 aggtcatcgt gatcgtcgcc atcggcttcg tggtctggtt ctggatcctg tcgatatatc
     2161 ccgtctccca catggcttcc ttcagcctgc tggcgccggt gttcggggtc ttgttcggat
     2221 ggctgatctt cgatgatcag attacgccag cgttcgtgat cgccttgctg ctggttggat
     2281 ctggtatcgt gctgatcaac cgcagcccgg caggatgagt ggatggacaa cggcagatgg
     2341 acggtcgcgt cgcgcccgga ggaccaggtg gaagaggcca atttccagtt cgtcagcgag
     2401 tcggtgggcg agccggagga cggcgagttt ctcatcggca cgcgctatct ggtggtgagc
     2461 ccgccggccc ggatggcgct cacctctggc ggcatcgcgc gccgtccagt gccgatcggg
     2521 gccaccatgc gcggaacggg gctgggccag gtggtcgcct cccgccatcc ggggtttgcc
     2581 gagggcgaac tcgtcatcgg ggatctcggc tggcaggagt acgccatctc ggacggcgcc
     2641 acgcgccacc ccgtgcggaa ggtggcagcc agaccgggga tgccggaaag caccctgctg
     2701 catgtgctgg gggccggggg tgcaaccgcc tacttcggca tgatcgagta tgccaagccg
     2761 cacgtgggcg acacgctggt ggtctcggcg gcggcgggca acgtcggctc gctggtctgt
     2821 cagctgggcc ggctgcaggg ctgccgggta gtcgggatag caggcagcga cgagaagtgt
     2881 tcctggctca ccaacgatct aggctgcgcg gcggcgatca actacaaacg tgaagacgtc
     2941 gccgcagccc tggacggagc ctgccctgac ggcatagacg tctacttcga caacgtcggg
     3001 ggagagatcc tcgacgctgc gcttgcgcgc attgcccagg gcgccagggt ggtgctgtgt
     3061 ggcggaacgt ctcagtacaa ccacgacctg gactggtacg gaccgaagaa ctacttcaat
     3121 ctggtctatc gccaggctga aatggccgga ttctacgtct acaacttctc ccaccgcttc
     3181 gaggaggcct ttgggcggct ggagtccctg ctcgcgacgg gagagctcaa gtatgccgag
     3241 gacgtgctgc agggaatcgg gcagacgccg gcagcgctga ttcgcgtcct caaaggcgag
     3301 aacttcggcg tgcagctggt cagggtggcg tgacggctgc tgctgcgccc tcttccgcgc
     3361 gcagttcctc cagggccttg ccgcaggcgt cgcgggagcc gaaagtgcgc ttccaggtgg
     3421 cctgagcgag cagggacaaa cctccggtca atgccgccgc gccgcccgtc accgccgcgc
     3481 ttcggctgtc caggctcaag gtcggcttaa gcagggttcc actcacccgc acgtaggggt
     3541 tgatcagctc ggtggcgctg atactcagtc ggagtcttgg cagcgcgttg aaggtgaaat
     3601 cgatgcgctc ggtggccagg ttggcgaatc cgctgctgac caggttcagc ttgtcggagc
     3661 gtatcgcgag gtacggatcg gctttcagaa tgccgtccgt tgcggagaat cgaaacacca
     3721 cacaggacaa atccgtgtaa ggatcatgtt tgatgaaagg attgatgttg ccgagcagct
     3781 cgcgcccgaa cccgccgaag atcagcgcga gctgtcggtt gtggatccgg cccccgtcgc
     3841 tcagcgcgct gacctcgcct tccagcgatg ctgccagagc ccgcgcatcg gtgcctcggg
     3901 cgcgcagata cacgtcgacg tcgaacacga gctggtcggc aggctccgcg gacggttcga
     3961 ggtttgccgc cacgttcagc gccacccgat caccgcggag ctcgaggtcg atggtgccgg
     4021 attcgtcttc cggccggacc gctagacggc cttgcacccg gccgctgggt gggcggatgt
     4081 cgaggcgttc caccagcaag ttgccgtcct ccagcacggc ggacaggaac ccctgggtaa
     4141 acgtcaggtc gccgaatctc agctcgtcca cggtcagttc gagcgcccca tccatgacgt
     4201 ccagaccgtc gaccggcaga gcgacgtcgg ggatcagccg accgtccgcc ggcggcgggc
     4261 gccgcgcctc ctgcgcgtct tcgctcaccg gcacgtccaa tgtcgggatg tggatctttg
     4321 cctgcagtcg cggccgggta ccgccgacgt ccagctgaag atcgccggag agcgctgagg
     4381 cgccgagctc cgcctcgatg ccttcgagcc tcagcgtggt gctcgtgccg ctggcgcgcg
     4441 cgctcaggct caaaggcgcg gccggcaacg gctcgccatc gagcctgccc agcgccgcta
     4501 gctcggggac gttcactgac agggtcgatt cggtggcgga gaactccggg ggcaggtcga
     4561 aggtgccgcg ccagtcgagc tcggcctctc cgaggaccag gctccccttg tctacggtga
     4621 gccggtcgcc gcgcaggctg gcgcgcagaa gcgcctcgaa cggcagcggc tcgagttcca
     4681 ggcgttgggg ttgaaccggt agcgcagaga cgtcgggccc ccgggccacc agattgacgt
     4741 ctgccgccct gctcgccggg aaaggtagcc cgagctccgc gttcaggcgc acctcgccgc
     4801 cgcgctcgtt cgtgagcaga aggtcttgga tctgaagcgt ttcgggcgtg cgctccagcg
     4861 tggcggtgac ttcgaagggg gccggcttga aatcgaggcc cgcggcgtcg gcgatcaggc
     4921 tttcgagctc tccgccgcgc ccggtgacgg tgacacggct gccgacgagg tcggccctgc
     4981 gggagagcga accttccagg tcgaatctgg attcctcgat ttcgaggtaa acggcgctca
     5041 gctcgaagac gcggcgcttt accgtcaagc gaccgccgga actgaacggc ttggcaggaa
     5101 ccgcctccag agcagtcggt tccaggcttt cagtcacctt caccagcgct ctgagatccc
     5161 tgccgctggc ggtcaggctc agctcggtgc cgccgtactc gggtggatcg ccgatggggc
     5221 ctcgcaggct gacgttggcc agcgaggtgg cggcggcaag gtccagggtg tccgtctggc
     5281 cttcggagtg ctcgatggaa cccgcaaccg taaagggacc caccgcccag tccggaaggg
     5341 agaggaaacg ctggaaccgg ccgagttccc gaccctcgag gcgaagcctc agggacgctg
     5401 catcgattcc cggaaatccg cgcgtctgcc cttcgatctc gaggcgggtg ccttccacct
     5461 ccagcagcac gtcgctcagc gtcaatacgg ggccggagcg ttttacctga cccgacgcct
     5521 ggaacggatc gtccggcagc ccctccagac cgaaaacgtc catcacgcgc ccgaagcggg
     5581 gtccctgaac gctcaggttc aggctcgatt cgcgcatcga gctcaagtcg tcggccgcgg
     5641 cgtcgaccct cacccggaga ccggccagat cgccggtcgc cgtcagcgcc actgtatcgg
     5701 tgcccggggt cagctcgatg ttcagatcca gcccgccatc tccgaactct tccgcgccca
     5761 gggcatcggt gatcaggtcg acgtcggggc tcatcaggtt caccttcagg gccggacggc
     5821 ggggtctccc gaggtcgtcc atgtgccccg aggcggtcag ggtcaatgct ttggcgatca
     5881 gggagagctc gaagtcgaag tcctccccgg cgatcacggc aggcagagga ctgacatggg
     5941 agctgacgac caccgagcga ccattgacgg tcgcctggag ggacccgctg aggccgtcgg
     6001 tctcagggtc gaaatgctgg cgggcgtcga ccacctcgat gctgagcggc ccctcgagtt
     6061 ccgggtccag gtaggtaacg gacagggcgt cgacgacgat gtcccccagc acgggcagcg
     6121 tcaacgagtc ggccgcgtcg ggctcatctg cagagattac ccagttgcca tcccggtcgg
     6181 ccgggttctc caggagcacg gaggcgtctt ccaactcgat gaggtggatg cgtgctggtc
     6241 cgcgcagcag ggagagcaga tccaactcca accggagccg cccgatgcgc gccatttctg
     6301 ggtcgctcgc ccattccgca ttgcccagcc gtacatcctc ggcaacgatc gccgtgcgcc
     6361 gccccgcgtt cagggaaaac tcaccgccga tggtgaaagc gcggcccgtc tgctccgcga
     6421 tcaggcgttc cacctggggc ttcaagaaac tcagatctgc ctgagtgagc cacacggcca
     6481 tcgccccgag ggcgacgacc acagcgagca ccgcaatcag tgtggcccgc agccaaccca
     6541 tgaacgcttc ccctcctcga acccaagccc gccggatgac gggacgaacg agggatttaa
     6601 cagagatggg cgtggcgggc aacgacgggt tacgatggcg caaagtcgaa gcgggacggg
     6661 cagatgaccg atcagttgaa gctggagatc ttcagcgact acatatgacc ctggtgttac
     6721 ctcagtaccg ggcgtattga aaaactgaag tccagcttcg atctggaggt tcgctgggtt
     6781 gccttccccc tgcatccgga aactcccgag gagggaatca ccatcgaagc gctcttcgcc
     6841 ggtcgcgaga tcgatctgga tgccatgtac gtgcggatga aagcgctcat ggacgaggag
     6901 gggcttcctt acggccgtcg tacccacacg ttcaacagcc ggctcgctca ggaactcggg
     6961 aagtgggccg actcccgccc cggtggcgaa gccatacacg acgccctgtt tcgcgcctat
     7021 ttcgttgacg ggctgaatat cggtgatgcc ggcgtgctcg tcgacatcgc gaagtccgca
     7081 ggtctcgatc ccggcggtgc ccgggaggtg atcgaccagc ggactttctc caatgccgtg
     7141 gacgaggatt ggaagcgctc gcggcgatac gggatcaccg gcgtgccgac gttcgtcgcc
     7201 ggtggctacg gcgtcgtcgg cgcgcaaccc tacgagaccc tcgagcatct ggttcaacgc
     7261 gcgctcaccg agtcgcgggc ttagctggat cgtcgcggcg cgtcagcgcc tggttcgccc
     7321 ggctatccgc ccggccgcct cggcgcgtcg cgccgtcgcc cggctgcgcg cctgtgttgc
     7381 ggtctcgacg ttccagtggg ccaccgcggg cgcgtcccag tccgcgccca ggatcaacgc
     7441 gtcctcgtag cggatggcgc cgtcagccgc ggtctccaga acgtatgcgg gcgtggacac
     7501 gtagagcact tccacggtcc ggtccgtttc gttgcacagc tgcagcaggg tgcctgcctc
     7561 ggtcagcacg gcggctccag tgggcacctc gagtcgggac ggttcgctct cccccggatc
     7621 ccgcatccgc gcggtgagcg tgcccgcggt gacgtaggtg atctgcgtca gcgccggatg
     7681 gacgtgaatg gcggaacgca cacctggccc tatccgcccg gcggccatgc tgatcgcttc
     7741 gcccgccgag tgcgcgctgg cagcgccgcg cgcgtcgtag ggattgagga aagcgcagac
     7801 ttccgtgccg tcagcggccg gcagaaatga ctcgggtcga aggatcgcgt ccgctggccg
     7861 gaagggcagc tcgccgggct gttcagggtc aggcatcgcg aatcgggtcg ctgacgccgt
     7921 tccaggcgtc gtggacggcc ttccagtggg cgaaggtctc ccgcagcggg gcgttgtcct
     7981 cgtagagctc cacgaagcag cccaggatgg gccgagtgtc catgtaggcc acccgggcgg
     8041 cggacgtcag ggtggtcacg gcgggatacc cggcggcttc gatgtcgtcg acgacgtacg
     8101 cgtactgaac caccgggtag cgtatcaggc tcattgctct ctcctcgcac gggtggtgtc
     8161 tttatacccc aatacccccc acccgttacc cacaggggcg gctgccggga ccgctgcgga
     8221 cgccggcaac ccctgtacaa tgcggcattt tggggaaacg tcgaatgctg caggaacgta
     8281 tcgagggcaa gtggatcgac tgcttcgcac gggtgttcga gcggtgcgcc gttgctgaga
     8341 acgaggaggt cgccatcctc tctgagagcc agagccggcc ggccaacgca cagctcgccg
     8401 agctcgccct gctcaggctc ggggccaggc cctaccatct ggtgatccct acgccgccac
     8461 agaaggcgcc ggtgcccgtt cgttccacgg gcgcaaccga cgtgattggc cactcgcgtt
     8521 ccgtcgtcgc cgcgctgtcg tcggcgggat tcgtcgtcga ctgcaccgtc gagggcatgc
     8581 ttcacgcgcc cgagctgccg gaaatcctca agggcggcgc ccgggtgctg atgatttcca
     8641 acgagcaccc ggaggccctg gaacggctgg agcccgacga ggccctggag acccgggtca
     8701 aggccggtat caggaagctg cgcggcgcga gccagatgaa ggtgacctcg gccgcgggga
     8761 cggacctcac cgtctctctc gaaggctccg tggccggcgg cggctggggc tacgttgcgc
     8821 gccccggcat catgagtcac tggcccggcg ggctttgcct gtgtttcccc gcggccggct
     8881 cggtgagcgg cgttctcgtt ctcgaccgcg gtgacgttaa cctgacgttc aagcgttacc
     8941 tggagaaccc cgtggcgctc agcatcgacg ccgactacgt cacggagatc gacggggcgg
     9001 gggtggacgc cgagctgatg cgcagctact tcgcggcgtg gggagaccag gaagcctacg
     9061 ccgccagcca tctcggttgg ggcatgaaca acgccgcccg ctgggacgcc atgaccatgt
     9121 acgatcgcaa cgacttcaac ggcacggagt tgcgagcgtt cggcggcaat ttcctatact
     9181 ccaccggcgc caacgaagtc gccgggcgcc ataccctcgg ccacttcgac ctgccgctgc
     9241 gcaactgctc cgttcacctg gacggcgagg ccgtggttgt ggacggtgcc ttgcagcgcg
     9301 agcttgcttg agcgttccag gtttcaagga agcgggttcc ggcgagccgg tggtgtttct
     9361 gcatggcatc ggcggcaacc ggcatgcctt cgacgcccag ctggccgagc cagggcgcgt
     9421 gcgttctctc gtcctcgccc agacgatggc gcgcttcggc aagccgggca gcgattggca
     9481 gcagcagttt ctggatgccc ggctcaagcc gctcgaggat ggccgtgccc cggcggactt
     9541 tgccagagaa ctgatcaccg gcatgttcgg cgatccgtcc cgtgcggaag ccatcgagcg
     9601 ggccgttgcc accatgagcc ctctgcccgc ggacgtttac cggcaggtgc tcacttgcct
     9661 ggtcgtcttc gatcagcggg gtacccttcc tgagatcgac gccccgacgc tgtgcctggc
     9721 agcggagctc gatcagacgg cgccgccgcg caccatggaa aagatggcga ccgagatccc
     9781 ggccggccgt tacgattgtc tgcaggacgc gggtcatctt gcgtacgtgg aaaggcccgc
     9841 tgctttcacc cgggccgtga acgaatttct gggtcagctg tgatgacgaa gactgaggaa
     9901 ctgatcggta ttgcccgcga gctgggcagg gaccgctttc tcccccgccg gcggacctat
     9961 gacgaggaag cgcgattccc ggacgagaac tacgaggacc tgcgcgaagc cggtttcctc
    10021 ggtctcgcca tcgaccggcg ctacgggggt ctcggcgccg atttcggcac ctacgccagc
    10081 atttccgttg agctcgggcg ctggtgcggc gccacggcgc tcaccttcaa catgcacact
    10141 tccaccatgc tctggtcggg tcagctggcc gacgacctgc ccatgagcga cgccgagcgc
    10201 tcggcccacg agtctcgccg agcggaaatc tatgccgggg tgatcgagga aggccacatc
    10261 ttcgcgcagc ccttctcgga gcccaacagc gccgccgcgg ccggcaaggc ggccttcgat
    10321 acatccgccg tgaaggtgga cggtggctgg ctggtgaacg gccgcaagca ctttgcgtcc
    10381 ctttccggct cggcgcactt ctacggcgtg ctctgcaccg acctcaccga cggccgcgag
    10441 ccggacgtcc gcaacacgct gctgctggcg gtgcctgcca gcgccagcgg gttctccgtt
    10501 gccggtgact gggacgtgct cggcatgcgc ggcacggtct cccgcagcct cgttctcgag
    10561 gacgtcttcg tgccggattc gggccagctt ctgccccagg gctgctacta ccaggcggcg
    10621 ctgggctggc cgcacatgtt catgaccctc tgccccacct acctcggcat cgctgaagcc
    10681 gccttcgatt tcacctgcca gtacctgcgc ggggaggtgg aaggcggccc gccggcgggc
    10741 ggcgcccgcg acaacccggt caagcaggtg ggcgtcgccg agatgcgcat caagctggag
    10801 caggcccggg cgctcttcga gaaagccatc ggggaggccg cctaccgacc gagcaaagac
    10861 gagcggctgc gcgcttacgc cgctcagtac acggtcatgg agtttgccaa cgacatctgc
    10921 cggcaggcca ttcgcgtgtg cggcgggcgc tcgctgttca aatccctgcg gctggagcag
    10981 ctttaccggg attcccgctg cggctcgctc atgctcccct ggacgcccga catctgcatc
    11041 gaacgcctcg ggcgggaaag tctggagagc tgaggcgttg aacggtctcg gcgactggat
    11101 ccgctgcaac gctgactggg caggcggccg cccggcgctc cgtctgggcg agactgcata
    11161 cacctacgcg gagctcgacg ccgccgtgga tcgcatggcc tttctgctgc gcgacggcct
    11221 gagcatccgg cgcggcgacg tggtggcgtt tctcggcctc aacagcgagc agatgatcgt
    11281 tttgctgttc gcgtgcgccc gcaacgcggc cagcctgctc cccctgaaca atcgcctggc
    11341 cggcccggaa cacgagtgga tactgcgtca cagcggcgcg cgctttctct tcgttgaaga
    11401 cgagtttgct gcccaggcga acgcgatcga tgtgccgggc ctggagcggg tggccatggg
    11461 tgcgccgccg gagccacatt ggcacgagtt tcagcgcatg ctcggtgacg caagggaagg
    11521 cggtttcgaa gacgtgggaa cgctggatga tcgggtgctg ctgatttaca cctccggcac
    11581 caccggccag ccgaagggcg tggcgcacga ccagcgtgcg cttttctaca acgcgctgaa
    11641 cgcgattcac gctcaggaga tgcgggcgtc ggaccacgtg ctcaccgtcc tgccgctctt
    11701 ccatgcgggc ggtctcaaca ttcagaccac gccggctttt tacattgggg ccggcgtgag
    11761 cctgatgcgg cgcttcgatc cgggtgaggc cctcagggcc atcgagacgc tcaagcccac
    11821 catgttcctg gcggtaccgg cggtcatcac cgccatggtc gaccacccgg cgtggtcgga
    11881 gaccgacctc tcctccctgc gtatggtggg caccggctcg tctaccgtac cggacccgct
    11941 gctcgacgct tggcacgacc gcggggtgcc cgccacgcag atatacgggc tcacggagag
    12001 cgcgccgacg gccatctgcc tgcccatcgc cgagacccga cgcaagcggg gggcggcggg
    12061 caaaccggtc attcactgcg aggcgcggat cgtggacgat gggggaaaca tcctcgggcc
    12121 gggggagcgt ggcgagatcg ttctgaaagg tccgaatctt ttctgcgagt atctgaatga
    12181 cccggagacc acggccgccg cgttctccga cggctggttt cataccgggg acatcggcca
    12241 catggacgcc gaagggttct tctacgtgga cgatcggaag aaggacgtgg tcatctccgg
    12301 cggtgagaac gtctaccccg ccgagctcga gaacgtgctg gcggagatgc ccgagctcgc
    12361 agagtacgcg gtggtgggcc ggccggacga gcgctggggc gaggtcccgg ttgcctgcgt
    12421 ggtgctgcgg gacggggccg acctgggtga agatgagctg atcgcgcgct tcgccggacg
    12481 tcttgcccgt tacaagcatc cccgggaagt gcgcttcttc cagtcgctgc cgcgcaacgt
    12541 catgggtaag gtgctcaagt tcaggctgcg cgaggaggtc gccccctgac cctctaggcc
    12601 gctgccagcg ccgcttcctt cccctgcccg gtttcctccc ggtattccga gaagtcggga
    12661 ttgcgcatgg acttcagata gcgcatcggc tcccaggggt acaggttcgg gatcccggtc
    12721 ttgtcgatgt accagctcgt gcagccgccc gtcatccaga ccgtgttctt caccgcctcc
    12781 tgcatggcct ggttgtagtt ccggtaggcg tcctcgcgcg cggagacggc cacgagccgg
    12841 cgcttcttca tgcgctcgag catcgcgatg acgtagtcca cctgatgctc ggtgatggtg
    12901 atcaaggaca tgttgcccac ggggcccgta gggccctcca gcatccagaa gttgggaaag
    12961 cggggaatcg ccacggctct cagtgctcgt ggggcgccct gccagaagga ctccaggtcc
    13021 accccgtctt cacccgtcac cttggtcggc agaatgaacg cgctcggatc gaacccggtg
    13081 gccaggacga gaacgtcgag ctcgtggacg cgaccgtcgg cggttcggat gccggcgggg
    13141 gtgacgcgct cgatggcgtc cgtgaccagg tgggcgttgt cgcgggtgat ggctgggtag
    13201 aagtccgagc agaggatgag gcgcttgcag gccgcctggt agtcgggcgt cagcctgcgg
    13261 cgcagctccg ggtcctttac gttgcgctcg aggttctgcc gggtgatttt gctgatgcgg
    13321 gcctgcatct tctcatcgcc cacggtggcc atggcgaagg tgcgaaccat gaggcggaag
    13381 tagtactgat aggccaggcg cttcaagaac gggaagagct tgagcacccg cttccagcgg
    13441 gcgctgtagg tcttctgcgg catgggcacc atccactggg gcgttctctg gaatacgtgg
    13501 agctcgccaa cggagccggc gatgtcgccg acgatctggg ctgccgtgga cccggtgccg
    13561 atcacgccca cacgcttgcc cgtgagatcc accgagtgat cccagcgggc cgagtgaaag
    13621 ctggctcctg cgaaatcctc cagtccttcg atgtccggca tggcgggatg gtggaggatg
    13681 cccgtggccg agatcacgac gtcgtagatc tgctgctcgc ccgcggcggt agtgaggcgc
    13741 cagaacggtg cttcgtaggt gagctccgtc accggggtgt cgaaccggac cacgtccgtc
    13801 acgccgaagc cgtcggcgac cttctccatg taggcctgga tctccgggcc gtaggagaat
    13861 cggtgagtcc actcggagct cagggcaaag ctgaacgagt accagcgtga gggcacgtcg
    13921 caggacagcc ccgggtaggt gttctcgcgc caggtcccgc cgacccggtc ggccttctcg
    13981 taaacggtga ggtgggtgta accggctttg cggagcttga cgacggcggc gatgccggac
    14041 atgccggccc cgatgatggc gatgcggggc tggcgatcgg ccaccgccca gggttccagc
    14101 tgagatgcca gtacgttcat gcggcccccg cctttattga cacatcgtca ataaaatagg
    14161 ttatatattg acatcgtgtc aatagtggat tgcccggggt agaatgtccg ggtggaagac
    14221 tgaagcattc cgggaatgga ggtcatgaac gtcaagagca cgcgggtcag gcgcaatccg
    14281 gaggcggcgc gggcggagat cctcgcggcg gccatggccg ccctgcagga cgtccgcttc
    14341 tccgagctga ccgtggattc cttgatgaag cggacgggca tgacgcgttc ctctttctac
    14401 cactactttt ccggcctgga cgagattgcg ctgggtatgc tcgaggagtt cgcggcggac
    14461 attcgccgca cggtagatcc ctggctcacg ggggaactgg atgagcccga tcaccgcgcc
    14521 gcgacgctta cccatctcac ctcgatgttc gaggtgttcg aacggcacgc gcccagcgtt
    14581 gccgccgttg cccaggcggc cggcgggagc caggaggtct atcaggagtg gcagcggggg
//
LOCUS       DBNHKV010000411         5681 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_343320, whole genome shotgun sequence.
ACCESSION   DBNHKV010000411 DBNHKV010000000
VERSION     DBNHKV010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_343320"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1132
                     /gene="egtD"
                     /locus_tag="AC16PP_13350"
     CDS             158..1132
                     /gene="egtD"
                     /locus_tag="AC16PP_13350"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052706 - L-histidine
                     N(alpha)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HIR6482661.1"
                     /translation="MKQARRKARNVMFRDEHPDLGDSRREILAGLQRDQKAVNPKWFY
                     DERGSQLFDQITRLPEYYPTRTEVGILQENRREISACCGSGCLFIEPGSGSSEKVRIL
                     LETLKPSAYVPVDISAGFLEEAALKLGDEYPWLDIYAVCADFNRSWSYVDDLPEGKRV
                     VFYPGSTIGNLEPEAAVGFLRRVREITGDSGGVVIGVDTHKSTERLNAAYNDAAGVTA
                     DFNLNVLRRMNDLLDAEFDEGSFRHHAFYNTELKRIEMHLVSERAQAVRCNGATVEFA
                     EGETIHTENSYKYTVEDFAELVDAAGLELQQSWTDADELFSVHYLRVA"
     gene            complement(1148..1660)
                     /locus_tag="AC16PP_13355"
     CDS             complement(1148..1660)
                     /locus_tag="AC16PP_13355"
                     /inference="COORDINATES: protein motif:HMM:NF016162.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HIR6482662.1"
                     /translation="MWKFTGKSRPEFAEEPGPGQESVWDYPRPPALDPSDEQVEVRAD
                     GTPIASSRRTLRVLETAGPPTYYIPEQDIDWSQLTEIAQTTYCEWKGHASYYALASDP
                     ARVPVAWLYADPSDSFRAIDRHASFYPGRVECLVNGERVRPQPGEFYGGWITDRVVGP
                     FKGEPGTGHW"
     gene            1816..2337
                     /locus_tag="AC16PP_13360"
     CDS             1816..2337
                     /locus_tag="AC16PP_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482663.1"
                     /translation="MSIVELGAIGEFLGAFAVVITLVYLGLQVRHNTTSTRAGTLAYA
                     TDAWSDYLQRQSVDDLNLLLGLSIEPDELSSAEFMRAYYLYRVMFRRMESDFYQYRSG
                     ILDADTWDAYVESWRGDVFITPGGRAMWKLQRDYFGPEFRSAFDDVEASTPRTPQHLR
                     RRFAELWEAERPV"
     gene            complement(2399..3103)
                     /locus_tag="AC16PP_13365"
     CDS             complement(2399..3103)
                     /locus_tag="AC16PP_13365"
                     /inference="COORDINATES: protein motif:HMM:NF013223.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriorhodopsin"
                     /protein_id="HIR6482664.1"
                     /translation="MDKLFLQSGDFVGVTFWLVSIAMIAATVFFLYEGVKVKPQWRLS
                     LLVAGLVTLVAGVHYDYMRDYWVVEQATPIVYRYIDWLITVPLLMIEFYIILRAAGAA
                     LGHGSFVRLLVGTLVMLIFGYLGEAQVLNIHVGFWIGMVGWAIIIYEIFGGAAGKAAS
                     ASAQAVQSAFSAMRLIVLVGWAIYPIGYIWGYMLGGVDEGALNMVYNLADFVNRILFG
                     LIIWNLAVKDSGEAGH"
     gene            3249..3833
                     /locus_tag="AC16PP_13370"
     CDS             3249..3833
                     /locus_tag="AC16PP_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482665.1"
                     /translation="MGVFEYVAGFVAIVVGLAVARVMGGIGAFILSDRRAAMDWLLAG
                     WCLALIVTLIGWWMLAWFTLGQLETITYGAVFGWFAATALLYLASYILVPAGGAAFGG
                     AGAALGELRPAFFVCLAVHFAANIVESAVTGSLLPAQGLVVLMVVLSGIGAFLRGARA
                     HALLLLTWMVCMLVLNFVAVPAVGVNSGSVLEAG"
     gene            complement(3873..5639)
                     /gene="xsc"
                     /locus_tag="AC16PP_13375"
     CDS             complement(3873..5639)
                     /gene="xsc"
                     /locus_tag="AC16PP_13375"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF005713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HIR6482666.1"
                     /translation="MKMTTEEAFVKVLQMHGIEHAFGFIGSAFMPISDYFPRAGIKFW
                     DVAHECSGGYMADGYTRTTGKMAMIIAQNGPGITNFVTCVKTAYWNHTPMLLVTPQAA
                     NKTIGQGGFQEIEQMKLFEDMVCYQEEVRSASRMAEVLNRVILQAKRHSAPAQINVPR
                     DLFTQVIDIELPPIIDFERPSGGTRAIDEAAALLSKAEFPVILNGAGVILADAIGDSA
                     ALAERLEAPVCCGYQHNDAFPGSHPLFAGPLGYNGSKAGMELIAHADVVLALGTRLNP
                     FSTLPGYGIDYWPKDAAIIQVDINPSRIGLTKPLSVGIVGDAGDVARALLERVEEVSD
                     EERDARRNLIARTKANWQQELAGMDHEDDDPGTTWNERARADKPDWMSPRMAWRAIRS
                     VLPTEAIISSDIGNNCAIGNAYPTFETGRKYLAPGLFGPCGYGLPSIIGAKIGCPDVP
                     VVGFAGDGAFGISVTELTAIGREEWPAITMIVFRNYQWGAEKRNSTLWFDDNFVGTEL
                     DEQVSYAGIAQACGLLGVQARTMDELSAALGEALDNQMNHGRSTLIEAMINQELGEPF
                     RRDAMKKPVEVAGIDPADMRRA"
BASE COUNT          957 a         1735 c         1894 g         1095 t
ORIGIN      
        1 gcaagttcat gagcaatcag tgggttctgc gaggaggctc ctgcgtgacc aagtccagcc
       61 aggcgaggcc aacttaccgc aattttttct atccccagga tcgctggcag ttcagcggca
      121 tccgcctggc ccgctccgtg gagggcgaac gacggttatg aagcaggcgc gcagaaaggc
      181 tcgaaacgtc atgttccggg acgagcatcc ggatctcggc gacagccgcc gcgagatatt
      241 ggccggtctc cagagagatc agaaagcggt caacccgaag tggttctacg acgagcgggg
      301 ctcccagctg ttcgatcaga ttacccgcct gcccgagtac tacccgaccc ggacggaggt
      361 ggggatcctg caggagaatc gtcgcgagat cagcgcctgc tgcggcagcg gttgtctgtt
      421 catcgagcct gggagcggtt ccagcgagaa ggtcaggatc ctgctcgaga ctctcaaacc
      481 gtcagcctac gtccccgtgg acatctccgc cggtttcctc gaggaagccg ccttgaagct
      541 cggcgacgag tacccctggc tggacatcta cgcggtctgc gcggatttca atcggagctg
      601 gtcctacgtg gacgacctgc cggaaggtaa acgggtggtt ttctaccctg ggtcgaccat
      661 cggcaacctt gaacccgagg cggcggtggg gtttctgcgt cgggttcgcg agatcacggg
      721 cgattcgggc ggcgtcgtca tcggtgtgga cacgcacaag tcaaccgaac gactgaacgc
      781 ggcgtacaac gatgcggccg gcgtcacggc ggacttcaac ctgaacgtgc tgcggcggat
      841 gaacgacctg ctcgatgccg agttcgacga ggggagcttt cgccaccatg cgttctacaa
      901 caccgagctg aagcggatcg agatgcacct ggtgagcgag cgggcccaag ccgtgcgctg
      961 caacggtgcc acagtcgagt ttgctgaggg agagaccatc cacacggaga actcgtacaa
     1021 gtacaccgtg gaggactttg cggagctcgt ggacgcggcc ggcctggagc tgcagcagag
     1081 ctggacggac gccgacgagc tcttcagcgt tcactacctg agagtggcct gacccgaagc
     1141 ccgaggtcta ccagtgcccg gtgccgggtt ctcccttgaa cgggccgacc acgcggtcgg
     1201 tgatccagcc cccgtagaac tccccgggct ggggccttac ccgctcgccg ttgaccaagc
     1261 actcgacccg ccccggatag aacgaggcgt gccggtcgat ggcccggaag gagtcggaag
     1321 ggtcggcgta aagccacgcg acgggcaccc tggcggggtc ggatgcgaga gcgtaatagc
     1381 tggcgtgacc cttccactcg cagtaggtgg tttgggcgat ctccgtcagc tgactccagt
     1441 cgatgtcttg ctctgggatg taataggtgg gcggccccgc cgtctccaga acccgcagcg
     1501 tcctgcggct tgatgcgatc ggcgtcccgt cagccctcac ctcgacctgc tcgtcgctcg
     1561 ggtccagcgc tggtgggcga gggtagtccc agacggactc ctggccgggt cccggttcct
     1621 cggcaaactc cggtctgctc tttcccgtaa atttccacat ggccgaggat actaacaggt
     1681 gttcaacccg gccggcatgg ggcgctagcc cgtcgacggc ggttcgtggt cgatgccgtg
     1741 cttccctgga gatcgggact cggccaaacg cctgagcttc gttacgcttg gcatgccgta
     1801 attgaggagc cattcatgag catcgtagaa cttggcgcga ttggggagtt tctgggcgcg
     1861 tttgccgtgg tcatcactct ggtctacctg ggtctgcagg tccgtcacaa caccacgtcg
     1921 acccgggcgg gcacgctcgc ctacgcgacg gacgcctgga gcgactacct gcagagacag
     1981 tcggtggacg atctgaacct gctgctgggg ctttcgatcg agcccgacga gctgtccagc
     2041 gcggagttca tgcgcgccta ctatctgtat cgcgtgatgt tccggcggat ggaaagcgac
     2101 ttctatcagt atcgcagcgg aatcctcgat gcggacacat gggatgctta cgtggagagc
     2161 tggcgcggcg acgtgttcat cacgccgggc ggacgcgcca tgtggaaatt gcagcgcgat
     2221 tattttggac cggagtttcg aagcgctttc gatgacgtcg aagcgagcac gccccgcacg
     2281 cctcagcacc tgcgccgccg gttcgcggag ttgtgggagg ctgagcggcc ggtttgacct
     2341 tctgctctca gccgaacggg ccccaccccg ggtggggccc tcaggtggct gggctaactc
     2401 agtgtccggc ttcgcccgag tccttgacgg cgaggttcca gatgatcaga ccgaacagga
     2461 tcctgttcac gaagtcggcc aggttgtaca ccatgttcag cgcgccttcg tccacgccgc
     2521 cgagcatgta gccccatatg tagccgatcg ggtagatggc ccatccaacc agcacgatca
     2581 ggcgcatggc gctgaaagcg gactgcaccg cctgcgccga ggcagatgcc gctttgccgg
     2641 cagctccgcc gaatatctcg tagatgatga tggcccaccc gaccatgccg atccagaagc
     2701 cgacgtgaat gttgagcacc tgggcttcgc cgagatagcc gaagatgagc atcaccagcg
     2761 tgccgacgag aagccgcacg aacgacccgt gtcccagggc tgcacccgcc gcgcgcagga
     2821 tgatgtagaa ctcgatcatc agcagcggaa cggtgatcag ccagtcgatg tagcggtaga
     2881 cgatgggcgt cgcctgctcg acgacccagt agtcgcgcat gtagtcgtag tgaacgccgg
     2941 cgaccagagt cacgaggccg gcgaccagaa gtgagagccg ccactgcggt ttgaccttga
     3001 cgccctcgta aaggaagaag acggtggcag cgatcatcgc gatagacacc agccagaagg
     3061 tgacgcctac gaagtcgccg ctctgcagaa agagcttgtc cattgttaat ccctgtaaat
     3121 tgcgttattg cttgagtttt actgcatcgt ggatgtcaac agggctcacg tgaaagctgc
     3181 gtgaacgggt ctgtcatcac tggtgatgct ggatcgggaa aggccagggt gtgcgacggg
     3241 gagttgccat gggcgtgttc gaatacgtag ccgggttcgt tgccatcgtc gtcggcctcg
     3301 ccgtcgcgcg ggtcatgggt gggatcggcg ctttcattct gtcggatagg cgcgcggcga
     3361 tggactggct cctggccggc tggtgcctgg cgctgatcgt cacgctgatc ggatggtgga
     3421 tgctggcctg gttcaccctg gggcagctgg agaccatcac ctacggcgcc gtatttggtt
     3481 ggttcgcggc gacggcgctg ctctatctcg cgagctacat tctggttccc gctggaggag
     3541 cggctttcgg cggagccggt gcggccctgg gcgaattgcg gcccgccttc ttcgtctgcc
     3601 tggccgtgca ctttgctgcc aacatcgtcg agtccgctgt tacaggttct ctcttgccgg
     3661 ctcaggggct ggtcgtgttg atggtcgtgc tctcgggaat cggcgcgttt cttcgcggcg
     3721 cccgggcaca cgccctgctg ctgctaacct ggatggtctg catgcttgtc ctgaacttcg
     3781 ttgcggtccc ggccgtgggg gtaaactcgg gctcggtgct ggaagcaggc tgagccgaac
     3841 gcgcccgctg cccggccagc cccgattcca gcctaagcgc gtcgcatgtc cgcgggatcg
     3901 atgcctgcga cctcaaccgg cttcttcatg gcgtcgcgcc ggaagggctc gccgagctcc
     3961 tggttgatca tggcctcgat gagcgtcgac ctgccgtggt tcatctggtt gtccagcgct
     4021 tcaccgagag cggcggaaag ctcgtccatg gttctggctt ggactccgag cagcccgcag
     4081 gcctgggcga tgccggcgta gctcacctgc tcgtcgagct ccgtgccgac gaagttgtcg
     4141 tcgaaccaga gcgtcgagtt gcgcttctcc gccccccact ggtagttgcg gaagacgatc
     4201 atggtgatgg ccggccactc ctcgcggccg atggcggtga gctcggtgac cgagatgccg
     4261 aaggcgccgt ccccggcgaa gcccaccacc ggcacgtcgg ggcagccgat cttcgcgccg
     4321 atgatcgaag gcaggccgta gccgcagggg ccgaacaggc cgggcgcaag atactttctg
     4381 ccggtctcga aggtggggta ggcgttgccg atggcgcagt tgttgccgat gtccgagctg
     4441 atgatggctt ccgtgggcag cacgctgcgt atcgcccgcc aggccatgcg ggggctcatc
     4501 cagtccggct tgtcggcgcg cgcgcgctcg ttccaggtgg ttcccggatc gtcgtcctcg
     4561 tgatccatgc cggccagctc ctgctgccag ttggccttgg ttctggcgat caggttgcgc
     4621 cgcgcgtcac gctcttcgtc gctcacctcc tccacgcgtt ccagcagggc ccgggcgacg
     4681 tcgcccgcat cacccacgat gcctacgctc aggggcttgg tgaggccgat gcgtgacgga
     4741 ttgatgtcga cctgaatgat ggcggcatcc ttcggccagt agtcgatgcc gtaaccgggc
     4801 agggtcgaga acgggttgag cctcgtcccc agggcgagca ccacgtcggc gtgggcaatg
     4861 agctccatgc cggccttgga tccgttgtac cccagcgggc ccgcgaacag ggggtgcgag
     4921 ccgggaaagg catcgttgtg ctggtatcca cagcacacgg gagcctccag gcgctcggcc
     4981 agggcggcgg aatcgccgat ggcatcggca aggatcacgc cggcgccgtt caggatgacg
     5041 gggaactcgg ctttcgacag cagggcggcg gcctcgtcaa tggcccgcgt accgccgctc
     5101 gggcgctcga agtcgatgat cggcggcagc tcgatgtcga tcacctgggt gaacaggtct
     5161 ctcggcacgt tgatctgggc gggtgccgag tgtcgcttgg cctgcaggat gacgcggttg
     5221 agcacctcgg ccatgcgcga ggcggagcgg acctcctcct gatagcagac catgtcctcg
     5281 aacagcttca tctgctcgat ctcctggaag ccgccctggc cgatggtctt gttggccgcc
     5341 tgcggggtga ccagcagcat cggggtatgg ttccagtagg cggtcttcac gcaggtgacg
     5401 aaattggtga tgcccggccc gttctgggcg atgatcatcg ccatcttgcc ggtggtccgg
     5461 gtgtagccgt cggccatgta gccgccgctg cattcatgcg ccacgtccca gaacttgatc
     5521 ccggcgcgcg ggaaatagtc gctgatcggc atgaaggccg acccgatgaa gccgaaggca
     5581 tgctcgatgc catgcatctg gagtactttt acgaaggctt cctcggtggt catcttcatg
     5641 ggggcggttc cttcttgtct gagtggggca cggggccagc g
//
LOCUS       DBNHKV010000412        12069 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1121713, whole genome shotgun sequence.
ACCESSION   DBNHKV010000412 DBNHKV010000000
VERSION     DBNHKV010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 12069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12069
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1121713"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="AC16PP_13380"
     CDS             <1..395
                     /locus_tag="AC16PP_13380"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6482667.1"
                     /translation="YLGDLFNKRLICGVCMFAHAGGLLLVTYADSIVGVLAFTLLHGL
                     AWGTRGPLMVSLRADYFGPTSFGTIMGFSSLIVMLGMSGGPIIAGIMADVSGDYHSGF
                     TLLASLSFMGSLCFFAATPPSPPARASA"
     gene            458..1147
                     /locus_tag="AC16PP_13385"
     CDS             458..1147
                     /locus_tag="AC16PP_13385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HIR6482668.1"
                     /translation="MPEFQGFPPRTLKFLKALERNNDRDWFRENKALYERDVLDPSLD
                     FIATMGPKLAAISDHFVAIPKRTGGSLMRVYRDTRFSKDKTPYKTNIGIQFRHELGKD
                     VHAPGYYLHIDTAGCFVAAGMWRPDAPSLARVRELIAERPGDWQKARDARPFKAAFEL
                     GGESLKRPPKGFAPDAPHLQDLRRKDFIASANVATGELGQPQLVDVVAELYARATPFM
                     RFLCRAIEVEF"
     gene            1327..2268
                     /locus_tag="AC16PP_13390"
     CDS             1327..2268
                     /locus_tag="AC16PP_13390"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR6482669.1"
                     /translation="MMNNTRLLVALAWLSLAPAGWAEDECTPSRWGSDDEIGNVNLIT
                     TQSVLAASRLIKTGKTYSLGIVVDGNTPAFPPRSLSLQIVQPGQQEGNRPLSRMTYND
                     DVFQGWFGIGSQLDGLGHAGDEGMYYNCNHARDFSDIAGLTKLGIEKVPPIVARGIVL
                     DMTAHFGVEHLAGGQTFSVDDVRAVEKKQGTPVREGDVVLFHTGWTDAMLETDPAGWS
                     ASAPGISKAVAGYLAGRNVVAVGSDTWGLDVLPPEREDEPFISHLILLQQNGIYILET
                     MNTGALVRDEAHEFLFVLGQAKVRGAVQMFINPIAIR"
     gene            2296..2793
                     /locus_tag="AC16PP_13395"
     CDS             2296..2793
                     /locus_tag="AC16PP_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6482670.1"
                     /translation="MTVEVNGIAHIQLTVRDPARCVPFWERLCHFLEMSTLIRAEEIV
                     YCIGSRTGVLVRGAPEDKRDRAFDQDRPGLHHLCFRARSREDVDAIYEFVAGELDAHV
                     VHPPEDGSHFAPGYYSVLFEDPDGIRVEVNFVPGRGHFGSEGRLGPGGRGPASRYGEE
                     GLGDG"
     gene            complement(2790..4331)
                     /locus_tag="AC16PP_13400"
     CDS             complement(2790..4331)
                     /locus_tag="AC16PP_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HIR6482671.1"
                     /translation="MLEDLLSKLPLEPVPVIVCAGIAVGCLLLGWICKAIGANRKLGQ
                     LRKEALEAKRAVPQLESSVHNRDLQIARFKLEAEDLTARNEELSTQLSTTNKELRSAR
                     REARNLNTELKAIKGHQSSLDNEVIAGFEEEDPLASADDQLKARMGRVESLYERVKEA
                     VIEREDRIQKLEEQLAQARNGNAAGGALLDDSDRDWTESTQELTEKVSSQEGLIESLR
                     AQIRKMQTEKEMLADMAKRRSDSNRALKEASAEAEAHVPVLRARLEERDRAIAENEAA
                     ISVLRDEVDMLRAQNAALEFESSGNVAADQPPVEDIFAIRKLLEDEFEAREEAFEKRL
                     REAEQAKEIAEADLALALAEQSKRVEEATSRIESLEAELGERRESTDVFKSVAEDREL
                     KLATLTNRVRVLLAAVNGDTATEHERLVELIRQLDPGHVQGPVPESSGTSDANGPARA
                     VKQAPPLPMRRAPRRPLRVIETRPESPPAPRGSRRAEVGRVLSALRPARSSVVPMRRR
                     GRMRR"
     gene            4627..5505
                     /locus_tag="AC16PP_13405"
     CDS             4627..5505
                     /locus_tag="AC16PP_13405"
                     /inference="COORDINATES: protein motif:HMM:NF014045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR6482672.1"
                     /translation="MARENEDRNLALFCDFENVALGVRDAKYARFDIQLVLERLLVKG
                     SIVMKKAYCDWDRYKEFKPVMHEAAFELIEIPHTRQSGKNSADIRMVVDALDLCYTKE
                     HIDTFVIISGDSDFSPLVSKLRENAKTVIGIGVKNSTSDLLIGNCDEFIFYDDLVRHQ
                     KATRKPRKKPAAKKAPEKKDATDATDAMDGKDAKEAKRAEPARSEEGNTDEGIALVLE
                     TAEALYGERGEREKLWGSMVKQTLKRRRPGFNESFYGFRSFSDLLEEAESRGLLELEM
                     DERSGGYLIRRLTREE"
     gene            5633..7786
                     /locus_tag="AC16PP_13410"
     CDS             5633..7786
                     /locus_tag="AC16PP_13410"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HIR6482673.1"
                     /translation="MNTSASDAAARSLAVIEETRQHGSIGHLPVWENITAAHVEAGVR
                     ALLDESEEAFTALETDVEASWAGLMEPLERLQTRIGQVIGAVTHLISVKYSDELQAAY
                     DAIRPIYVSLLNRMSQSLGVYGGLVALRDGPDWPALSQARQRIVTESIRAMERSGVHL
                     EGPPKERFKEIQERLSELGNDFSVNLVKEEKASRVRVQAPEEVAGVPAAVLGLAAAKA
                     REDGEDGATDASGPWHFVVNGVNFVAVMEHALDRGLRERFYRAFRARGTSRDFDNRCV
                     LTEILRLRQEQSALVGFECYADRSLDAKMAPDTDAVWQLFNELEAAARPAAEREFETL
                     KAYMRDEGGLDEGEQPAPWDLAFWSEKMRESLYAYDGERLREYFQLPRVLDGLFSLVG
                     RLYEVEVRRVAAGAVPVWDPAVEFFEVIGAGEVIAGFYVDPYARPGEKRGGAWMNTVV
                     SRSRVLAAPGKSSSLPVALFVMNARPPAEGKVALLSLDEVRTLFHEFGHATQHMFTTI
                     EDGGASGMNLVEWDAVELASQFNEYWMEHKPFLRDLTAHFDTGEPLDDETLDRIVDSR
                     NFMVGTATLRQLFFGKVDMTLHQRFGVAGSNDGASPFEIERDIADSTLVTPRLDDEST
                     LPAFGHLFAGGYAAGYYSYKWAEVLAADAFAAFKEAGLDDGAAMKEIAGRFRDTVLGL
                     GGSLPAGDVYRRFRGRDASVRALLADQGLVAAEAG"
     gene            complement(7817..8716)
                     /locus_tag="AC16PP_13415"
     CDS             complement(7817..8716)
                     /locus_tag="AC16PP_13415"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6482674.1"
                     /translation="MSAPPTRRRWQVADAFGDGNIEIAGLDWRGSGPLALLHHANGFC
                     AATWTLVARGLAGSHRVIAIDARGHGDSGTRPIPEGYQWDFFASDLTQVARQLLEETG
                     QPSVEFGIGSSMGGIVTAASEARNPGLFQRIAMLDPPLHPLPELRARLGLGVAAPNAD
                     LVAMTRKRKTIWPSRAIARQAWQDKPLFREWVPAAFDAYLEEGLGDRPDGTVALKCDP
                     GVEAAIFETTGSMDTFELAPRVSAPVLLVRALQGRAPGAAFEHLAGLLPRCTYREVDA
                     GHLLPMESPELTLALLAEFTEGG"
     gene            complement(8713..9540)
                     /locus_tag="AC16PP_13420"
     CDS             complement(8713..9540)
                     /locus_tag="AC16PP_13420"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6482675.1"
                     /translation="MPHVRINGARLWYEVRGSGEPLLLHHGYTASRVNWLPVAERLQD
                     RYQVVLMECRGTGDSEHTAEGYSLAQYAADVVGMLDHLDLERVTFAGHSMGGGIGYLL
                     GLDHAHRLDRLVLMAPIPAAGIAGLDPDARQKRLKLRRDQNRDALLAEYRAARFRPDV
                     ETDDWIESRVDHVLGVSDGHYEASAESMVELDVGHRLPELEVPTLMLAGAADELLRAN
                     LHDFLALPNATLEVLSRAGHEVAVHEPDAVARAIDAFMTHGPVTARHIRQQMEGESR"
     gene            complement(9564..9905)
                     /locus_tag="AC16PP_13425"
     CDS             complement(9564..9905)
                     /locus_tag="AC16PP_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482676.1"
                     /translation="MGNKLILVGLVEPQTDDLVEAFNQWYLGNHVEDTYNCPNITSVR
                     CFKAVRGFLGEPPSGYLTIYEFEGEDAEEAERILGQYQSDPDAWPEREPNNGSMKVVG
                     AGWYAEEIAFG"
     gene            complement(9922..10101)
                     /locus_tag="AC16PP_13430"
     CDS             complement(9922..10101)
                     /locus_tag="AC16PP_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482677.1"
                     /translation="MSVQNGERLIRFLRSDPQLREKVKDAGWEKFQDVTAEAGASCTT
                     YEVVAALIREIENDS"
     gene            10217..10738
                     /locus_tag="AC16PP_13435"
     CDS             10217..10738
                     /locus_tag="AC16PP_13435"
                     /inference="COORDINATES: protein motif:HMM:NF025050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6482678.1"
                     /translation="MSIIRHPIWQYAYFVNDIDEACHKWNRMVGAGPFHVVRHHIAEG
                     FLYRGEHVEADVSYAFGQAGPAHIQFIAQHDDTPSIYRDMYAPGEQGFHHIGLLVHDV
                     DGEIKRFNEAGYETACTLWGGDYVAYMDCRQDMGCYVELHGDAPIIRDLFDGWKRQHE
                     EWDGVTDLIREQG"
     gene            10744..11847
                     /locus_tag="AC16PP_13440"
     CDS             10744..11847
                     /locus_tag="AC16PP_13440"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6482679.1"
                     /translation="MEMRVLGRTGVSVSKLCFGAGMFGYFGNPDERECARMVDAAIDA
                     GVNYFDTSDVYSHGESESILGKALVGKRDDVILATKFSLPMSDDPNHRGNSRRWIARE
                     VEASLKRLQTDYIDLYQVHRPDVTADIDETLGALSDLVRSGKVRMLGSSTFPAEQIVE
                     AQWVAEKRHRERFVVEQPPYSIFARRIEADILPTCQRYGMGVVAWSPLSQGWLTGKWR
                     RDAPPNDTNRQKLQPHLYDMARADNQRKLDLVEQLAAVASEAGISLMHMAIAFVCAHP
                     AVTSAIIGPRTPEQLQGLLDGADVTLGDDVLDAIDAIVAPGTTLSRDDDGYVAPEIAN
                     KALRRRFVAEEVHEAARETLRNIEAYREQQGKK"
     gene            11844..>12069
                     /locus_tag="AC16PP_13445"
     CDS             11844..>12069
                     /locus_tag="AC16PP_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014861904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR6482680.1"
                     /translation="MSTTTALAAVAWAPDDPFAFEEVELGPLGADEVLVRIVASGICH
                     TDVAVKEQSVKLPLPMVLGHEGSGIVEAVGS"
BASE COUNT         1976 a         3853 c         4038 g         2202 t
ORIGIN      
        1 gctatctcgg cgatctgttc aacaagcgcc tgatctgtgg ggtctgcatg ttcgctcatg
       61 ccggcgggct cctgctcgtc acctacgcgg actccatagt gggcgtgctg gcgttcacgc
      121 tgctccacgg ccttgcctgg ggcacccgag ggcccctcat ggtctcgctg cgggccgatt
      181 atttcggtcc cacctctttc ggcaccatca tgggcttctc gtctctgatc gtgatgctgg
      241 gaatgtcggg cgggccgatc atcgcgggca tcatggccga cgtttccggc gactatcaca
      301 gcgggttcac cctgctggcc agcctgtcgt tcatgggttc gctgtgcttc tttgccgcca
      361 cgcctcccag cccgcctgca cgcgcatcgg catgacgctc gggccggaag ctgggtatgc
      421 tcccatcctt tctcgatgat tggtgaggag cgggccaatg cctgagtttc agggttttcc
      481 gccccggacg ttgaagtttc tgaaggccct ggaacgcaac aacgaccggg actggttccg
      541 ggagaacaag gcgctctacg agagggacgt tcttgacccg tcgctggatt tcatcgccac
      601 catgggcccc aagctggccg ccatatccga ccatttcgtg gcgatcccca agcggacggg
      661 cgggtcgctc atgcgcgtct atcgggatac ccgcttctct aaagacaaga cgccgtataa
      721 gaccaacatc gggatccagt tccgtcacga gctcggcaaa gacgttcacg cgcccggtta
      781 ctatctgcac atcgacaccg ccggatgctt cgttgccgcg ggcatgtggc gtcctgatgc
      841 gccgtcgctt gcccgcgtgc gggagctcat cgccgagcgg cccggggact ggcagaaggc
      901 gcgtgacgcc cgccccttca aggcggcatt cgagctaggg ggcgagtcgc tgaagcggcc
      961 gccgaaaggc ttcgcgccgg acgcgcccca tctgcaggat ctcaggcgca aggacttcat
     1021 tgccagcgcg aacgttgcaa ccggcgagct tggccagccg cagcttgtcg atgtcgtggc
     1081 ggagctttac gcgagggcga cgcccttcat gcgcttcctg tgccgggcca tagaggtcga
     1141 gttctgacgc ctacgcccga tggcttggcg gcccggcggt cgccgccggg ctctcccgat
     1201 ccgctctgcg gaatcgatcg ccgggatctt taccggatgc taaggtggac gatgctccgg
     1261 ggcggtggcg gtgtcagccg agccggtgcg cagcgtggga tttcaggccg gtcaacgaaa
     1321 ggggggatga tgaacaacac gcgattgctg gtggcgctgg catggctgag cctggctccg
     1381 gcggggtggg cggaagacga atgcacgccc tcgcgctggg ggagcgatga cgagatcggc
     1441 aacgtcaatc tcatcacgac ccagtcggtg ctggcggcgt cccggctcat caagacgggg
     1501 aagacctaca gcctcggcat cgtggtggac ggcaacacgc cggcgttccc gccgcggtcg
     1561 ttgtcgctgc agatcgttca gcccgggcag caggagggta accggcctct gtcgcgcatg
     1621 acctacaacg acgacgtgtt ccagggctgg ttcggtatcg gttcccagct cgacggcctg
     1681 ggccacgccg gtgacgaggg tatgtactac aactgcaacc atgcccggga cttctccgac
     1741 atcgccgggt tgaccaagtt gggaatcgag aaggttccgc ccatcgttgc ccggggcatc
     1801 gtgctggaca tgacggccca tttcggcgtc gagcatctgg ctggcggaca gacgttcagc
     1861 gtggacgacg tccgcgcggt ggagaagaag cagggcacac ccgttcgcga gggtgacgtc
     1921 gtgctgtttc atacggggtg gacggacgcc atgctcgaga cggacccagc cggctggtcg
     1981 gcctccgccc cgggaatttc caaagcggtt gccggctacc tcgccggcag gaacgtggtt
     2041 gccgtcgggt ctgacacgtg ggggctcgac gttctgccgc ccgagcgcga ggacgaaccc
     2101 ttcatcagcc acctgatcct tctgcagcag aacgggatct acattctcga gaccatgaac
     2161 acgggcgctc tcgtccgcga cgaagcccac gagtttctct ttgtgctcgg ccaggcgaag
     2221 gtccggggtg cggtccagat gttcatcaac cccatcgcca ttcgctgacg acggcggtcg
     2281 cgaaggagga gcttgatgac ggtggaggtc aacgggatcg cgcacatcca gctgacggtt
     2341 cgggatccag cgcgctgcgt gccgttctgg gagcggttgt gtcactttct ggagatgagc
     2401 acgctcatcc gggcggaaga gatcgtctac tgcatcggca gccgcacggg cgtcctcgtg
     2461 aggggcgccc ccgaggacaa gcgcgaccgg gcattcgacc aggatcgccc gggccttcat
     2521 catctgtgtt tccgggcccg ctcccgggag gacgttgacg ccatctacga gttcgttgcc
     2581 ggcgagctgg acgcgcacgt cgtgcatcct cccgaggacg gctctcactt tgctcccgga
     2641 tactactccg tgttgttcga ggatcccgac ggtattcggg tggaggtcaa cttcgtgcca
     2701 ggccgcgggc acttcgggtc agaggggcgt ctgggtcccg gcggccgggg accggcgagc
     2761 cgctatgggg aagagggtct gggagacggc tagcggcgca tgcggccacg tcgccgcatc
     2821 ggcacgacgc tcgaccgagc cggacgcaac gcgctcagca cccggccaac ctcggctcgg
     2881 cgggagccgc gcggtgccgg ggggctctcg ggacgggttt cgatcacccg cagcggtcgc
     2941 ctgggcgctc ggcgcatggg cagcgggggc gcctgcttca cagcccgggc aggtccgttg
     3001 gcatcggaag tgccggagct ttccggtacc ggtccctgca cgtggccggg atcgagctgc
     3061 ctgatgagct ccaccagccg ctcgtgctcg gttgcggtat cgccgttcac ggcggcgagc
     3121 agcacgcgca ccctgttggt cagcgtggca agcttgagct ctctgtcttc ggccacgctc
     3181 ttgaacacgt ccgtggattc gcggcgctcg ccgagctcgg cttccaggct ctcgatgcgg
     3241 gaagtcgcct cctcaacccg cttgctctgc tcggcgagcg cgagggcaag atctgcctcg
     3301 gcgatctctt tggcctgctc cgcctcgcgc agccttttct cgaaggcctc ctcgcgggct
     3361 tcgaactcgt cctcgagcag cttgcgaatg gcgaatatgt cctccaccgg aggctggtcc
     3421 gctgcgacgt ttccggaact ctcgaactcc agggccgcgt tttgggcgcg cagcatgtcc
     3481 acctcgtcgc gcagcacgct gatggccgcc tcgttctccg cgatggctct gtcgcgctct
     3541 tcgaggcggg cgcgcagcac gggcacatgg gcttcggcct cggcgctggc ctctttcagg
     3601 gcccggttgc tatcgctgcg gcgctttgcc atgtcagcga gcatttcctt ctcggtctgc
     3661 attttgcgga tctgggctcg cagggattcg atcagccctt cctgggagct gaccttctcg
     3721 gttagttcct gggtcgattc tgtccaatcc cgatcggagt catccagcaa ggcgccgcct
     3781 gccgcgtttc cgttgcgcgc ttgagccagc tgctcctcga gcttctgaat gcgatcttcg
     3841 cgttcgatca ccgcttcctt gacgcgctcg tacagcgact cgacgcggcc cattcgggcc
     3901 ttgagctggt cgtcggcgct ggcgagcgga tcctcttcct cgaagccggc gatgacttca
     3961 ttgtccaggc tgctctgatg gcccttgatc gccttgagct cggtattcag gtttcgggct
     4021 tctcggcgag cgctgcgcag ctccttgttg gttgttgaga gctgagtgga cagctcctcg
     4081 ttgcgagcgg tcagatcctc ggcttcaagc ttgaaccggg caatctgcag atcgcgattg
     4141 tgtacggaag actcgagctg gggcacggca cgcttggcct cgagggcctc cttgcgcagt
     4201 tgacccagct tgcgattggc gccgatggct ttgcagatcc atccgagcag caggcagcca
     4261 acggcgatgc ccgcgcagac gatgaccggc acgggctcaa gaggcagctt ggacaaaaga
     4321 tcttcgagca tgtgatcagc caaacatggt cctgcgtaat ggttcccgca atggttgggc
     4381 cattcagtta cctgtgtctc agcaggcacc ggcgccctcg ccgatccgca cgcttgtgcc
     4441 ccgaaatcgt gaacccgatc acgcccagtg ggggcggccc cgtcgggtcg gggaggcgct
     4501 gtcacttccg cttgcgtcga tctgagccct gcgatcccct ggtcgccgcc ttgcgtgacc
     4561 gtttgcttgt ggacggccgc acgcggcccc gttaacgtag gcgcaacctc agctgcggca
     4621 tacctcatgg cccgcgaaaa cgaagaccga aaccttgcgc tcttctgtga cttcgagaac
     4681 gtcgccctcg gcgtgcgtga tgcgaagtac gccaggttcg acatccagct ggtgctcgag
     4741 cgcctgctgg tcaagggcag catcgtgatg aagaaagcct attgcgactg ggatcgctac
     4801 aaggagttca agcccgtcat gcacgaggcg gcgttcgagc tgatcgagat tccccacacg
     4861 cgccagtcgg gtaaaaactc ggcggacatc agaatggtcg tggacgccct ggatctctgc
     4921 tacaccaagg aacacatcga tacgttcgtg atcatcagcg gcgactccga tttctcgccg
     4981 ctggtcagca agctcaggga gaatgcgaag accgtcatcg ggatcggcgt gaagaactcg
     5041 acgtcggacc tcctgatcgg caactgcgac gagttcatct tttacgacga tctggttcgc
     5101 catcagaagg cgacccgcaa gccacgcaag aagccggccg caaagaaggc gcctgagaaa
     5161 aaggacgcca cggacgccac ggacgccatg gacggcaagg acgccaaaga ggcgaagagg
     5221 gccgagcccg cccgctcgga agagggcaac accgatgagg ggatcgcgct ggtgctggag
     5281 accgctgaag cgctgtacgg cgagcgtggc gagcgggaaa aactctgggg ctccatggtg
     5341 aagcagacgc tcaagcggcg gcgcccgggg ttcaacgaga gtttctacgg cttccgctcg
     5401 ttcagcgatc tcctcgagga agcggaaagc cggggcctgc tggaactgga aatggacgaa
     5461 cgctccggcg gatacctgat ccgccggctg acccgggaag aatgacgcct tagaatgcgc
     5521 ttcccctcgc cgggacccgc cggacggacg aggggagaaa ccgcctcatc gtgtggacaa
     5581 acctcgctgc tcaaagcacg ccggccccga gaggcttgcc aaggagatca tcgtgaacac
     5641 atcggcatct gacgcggctg cccgcagcct ggccgtcatc gaggaaaccc ggcagcacgg
     5701 atccatcggg cacctgcccg tatgggagaa catcacggcg gctcatgtgg aggccggcgt
     5761 tcgcgccctg ctcgatgaga gcgaagaagc tttcaccgca ttggaaaccg acgtcgaggc
     5821 gtcctgggcc gggctcatgg agcccctgga acgcctgcag acgcgcatcg gtcaggtgat
     5881 cggcgcggtg acccacctga tcagcgtcaa gtactccgac gagctccagg cggcctacga
     5941 cgcgattcgc cccatctacg tgtcgctgct caatcggatg agccagagcc tgggcgtata
     6001 cgggggtctg gtcgcgttgc gggacggccc cgactggccg gcactgagcc aggctcggca
     6061 gcgaatcgtc acggagtcca tcagggccat ggaacgttcg ggcgtgcatc tggaaggccc
     6121 ccccaaggag cgcttcaagg agatccagga gcgcctgtcc gagctcggca acgatttctc
     6181 cgtgaacctg gtcaaagagg agaaagcgtc gcgggtccga gtgcaggcgc ctgaagaggt
     6241 tgccggagtg cccgcggccg tcctcgggct ggcagccgcc aaggcccggg aagacgggga
     6301 ggacggcgct acggacgcct ccggtccgtg gcacttcgtc gtcaacggcg tgaacttcgt
     6361 cgccgtcatg gagcacgcgc tggatcgggg gctgcgtgag cggttctacc gggcgtttcg
     6421 cgcccgtggc acatccaggg acttcgacaa ccggtgcgtg ctcacggaaa tcctccggct
     6481 tcgccaggag cagtccgccc tcgtggggtt cgagtgctat gccgaccgca gtctggacgc
     6541 caagatggca ccggacacgg acgccgtctg gcagcttttc aacgagctgg aggcagccgc
     6601 gcggcccgct gcggagcgcg agttcgaaac gctcaaggcg tacatgcgcg atgagggcgg
     6661 tctcgacgaa ggggagcagc ccgcgccctg ggatctggca ttctggtccg agaagatgcg
     6721 cgagtcgctc tacgcttacg acggtgagcg cctgcgcgag tattttcagc ttccccgcgt
     6781 tctcgacggc ctgttttccc tggttggtcg gctatacgag gtcgaggtgc gtcgcgtggc
     6841 ggccggtgcg gtgcccgtct gggatcccgc ggtcgagttc ttcgaggtga tcggggccgg
     6901 cgaggtcatc gctggctttt acgtggatcc ctacgcccgg cctggcgaga agcgcggcgg
     6961 cgcgtggatg aacaccgtgg tgtcgcgcag ccgggtgctc gccgcgcctg gtaagtcgtc
     7021 gtcgctgccc gtggccctgt ttgtcatgaa cgcgcgcccc ccggcagagg gcaaggtggc
     7081 gttgctgtcg ctggacgagg tgcgcacgct ctttcacgag ttcggccacg ccacccagca
     7141 catgttcacg accatcgagg atggcggcgc atcgggcatg aacctcgtcg agtgggacgc
     7201 cgtggagctc gccagccagt tcaacgagta ctggatggag cacaaaccct ttcttcgcga
     7261 tctcaccgcc cacttcgata ccggggagcc gctggacgac gagacgctcg atcggatcgt
     7321 cgacagccgc aacttcatgg tggggacggc caccctgcgg cagctctttt tcggcaaggt
     7381 cgatatgacc ctgcaccaga gattcggcgt cgcgggcagc aacgacggcg cgagcccctt
     7441 cgagatcgag cgggacatcg ccgacagcac cctcgtcacg ccgaggctcg acgacgagag
     7501 tacgctgcct gccttcggtc atctcttcgc cggtggctac gcggccggct actactccta
     7561 taagtgggcc gaggtccttg cggcggatgc ctttgcggcc ttcaaggagg cggggctcga
     7621 tgacggcgcg gcgatgaaag aaattgccgg gcgctttcgg gacaccgtcc tcggtcttgg
     7681 cggaagcctc ccggcggggg acgtctaccg gcgtttccgg gggcgggacg cctcggtacg
     7741 tgcgctgctg gccgatcagg gcctggtcgc ggccgaggct ggctgacgcg cccgagccta
     7801 agcggttgcc gacggctcag ccgccctcgg tgaactcggc aagcagcgcc agtgtcagct
     7861 cgggagattc catgggcagc aggtgtccgg cgtccacctc cctgtaggtg cagcgcggca
     7921 gaaggcctgc gaggtgctcg aaggcggctc cgggggcccg gccctgcagg gcgcggacga
     7981 gcagcacggg cgcgctcaca cgcggggcga gctcgaaggt gtccatagaa cccgtggttt
     8041 cgaagatagc ggcttccacg ccggggtcgc acttcagcgc caccgtccca tccgggcggt
     8101 cgccgagccc ctcttcgagg taggcgtcga atgcggccgg tacccattcg cggaaaagcg
     8161 gcttgtcctg ccacgcctgc cgggcgatcg cgcgggacgg ccagatcgtc tttctctttc
     8221 tcgtcattgc caccaggtcc gcgtttggcg ccgcgacgcc gagcccgagc ctggcgcgca
     8281 gctcgggtag cggatgtagc ggcgggtcca gcatggctat gcgctggaag agccccgggt
     8341 tgcgtgcttc cgaggcggcg gtgacgatgc cgcccatgga gctgccgatg ccgaactcga
     8401 cggatggctg ccctgtttcc tcgagcagtt gcctggccac ctgggtgaga tccgaggcga
     8461 agaagtccca ctggtatcct tcggggatgg ggcgtgttcc cgagtcgccg tggccccggg
     8521 catcgatggc gatgacccga tggctgccgg caaggcctct ggcaacgagc gtccaggttg
     8581 cggcgcagaa cccgttggcg tgatgcagca gcgccagcgg cccgctgcct ctccagtcca
     8641 gaccggcgat ctcgatgttc ccgtcgccga aggcgtcggc tacctgccat ctgcgacggg
     8701 tgggaggggc gctcatctgg attccccctc catttgctgc ctgatgtgcc gggccgtgac
     8761 ggggccgtgg gtcatgaagg cgtcgatggc ccgggccacg gcgtccggct cgtgcacggc
     8821 cacctcgtgg cctgctctgg agagcacctc cagggtggcg ttgggcaggg cgaggaagtc
     8881 gtgaaggttt gcgcgcagca gctcgtcggc tgcgccggcg agcatgaggg tgggtacctc
     8941 cagctctggc agtcggtggc ccacgtccag ctcgaccatg gactccgcgc tcgcctcgta
     9001 gtgtccgtcg ctgacgccga gcacgtggtc cacgcggctc tcgatccagt cgtcggtttc
     9061 cacgtccggg cgaaagcggg cggcccggta ctctgccagc agggcatcgc ggttctggtc
     9121 gcggcgcagc ttcaggcgct tctgccgcgc gtccggatcg aggccggcga tgcccgcggc
     9181 gggaatgggc gccatgagca ccaggcgatc cagccggtga gcgtgatcga ggcccagcag
     9241 gtagccgatg ccgccgccca tgctgtggcc ggcaaaggtc acccgttcca ggtcgaggtg
     9301 gtccagcatg ccgacgacgt cggccgcata ctgggcgagg ctgtatccct cagcggtgtg
     9361 ctcgctatct ccggtacccc ggcactccat gagaacgacc tgatagcggt cctgcagacg
     9421 ctcggccacg ggcagccagt tgacccgtga cgccgtgtag ccatgatgca gcagcagggg
     9481 ctcgccggag cctcggacct cgtaccacag acgggccccg ttgatgcgga catggggcat
     9541 ttttacggtc gccgcgctcg ggctcagccg aaggcgatct cctctgcgta ccagcctgcg
     9601 cccacgacct tcatcgatcc gttgttcggc tcgcgctcgg gccaggcgtc gggatcggac
     9661 tgatactgac cgagaatgcg ttccgcctcc tcggcgtcct cgccttcgaa ctcgtagatg
     9721 gtcaggtacc cggagggcgg ctcgcccagg aacccccgca cggccttgaa gcagcgcacc
     9781 gacgtgatgt tcgggcagtt ataggtgtct tccacgtggt tgcccaggta ccactgattg
     9841 aaggcctcga ccaggtcatc cgtctgcggc tctaccaggc ccacgaggat cagtttgttg
     9901 cccatgaatc gtctttcctg ttcagctgtc gttctcgatc tcgcgaatga gcgcggcgac
     9961 gacctcgtag gtcgtgcagg aggcaccggc ctcggcagtg acgtcctgaa acttttccca
    10021 tccagcgtcc ttgacctttt cccgcagctg cgggtcgctt ctaaggaaac gaatgagccg
    10081 ctcgccgttc tgtacggaca tgtccccccc cttttcgaga tgtcggtttc gtagccgagt
    10141 ctacagcact cggcggcgtc gggttagtct gctatcgttc ggcaaggttt gaggtggatt
    10201 tcaaagggga gacatcatga gcattatccg gcatcccatc tggcagtacg cttacttcgt
    10261 caacgacatc gatgaagcct gtcacaagtg gaatcgcatg gtgggcgcgg gaccgttcca
    10321 cgtcgtgcgg catcatatcg ccgaaggttt cctgtatcgc ggcgagcacg tcgaggcgga
    10381 cgtgagctac gcgttcggcc aggccggccc tgcacacatt cagttcattg ctcagcacga
    10441 cgacacgccc tcgatctacc gggacatgta cgcgcccggc gagcagggct ttcaccacat
    10501 cgggctgctc gtgcacgacg tcgacgggga gatcaagcgg ttcaacgagg cgggctacga
    10561 gaccgcctgc accctctggg gcggcgacta cgtggcctat atggattgcc gtcaggacat
    10621 gggttgctac gtggagctgc acggtgatgc gccgatcatt cgcgatctct tcgatggctg
    10681 gaagcgccag cacgaggagt gggacggcgt cacggatctg attcgcgagc agggctgagc
    10741 gtcatggaga tgcgggttct cggccgtacg ggcgtcagtg tcagcaagct ctgcttcggt
    10801 gcgggcatgt tcggctattt cggcaatccc gacgagcgcg aatgcgcgcg catggtggat
    10861 gccgccatcg acgcaggcgt caactacttc gacacgtccg acgtgtactc ccatggggag
    10921 tcggaatcca ttctcggcaa ggcgctcgtc ggcaagcgcg acgacgtgat tctcgccacc
    10981 aagttcagcc tgccgatgag cgacgacccg aaccatcgcg gcaactcgcg gcgctggatc
    11041 gcgcgcgagg tcgaggcgag cctgaaacgt ctgcagacgg attacatcga tctctatcag
    11101 gttcatcggc cggacgtgac cgccgacatc gacgagactc tgggcgccct ttcggatctg
    11161 gttcgctcgg gcaaggtccg catgctgggc tcgtccacgt tccccgcaga gcagatcgtg
    11221 gaagcccagt gggttgccga gaaacgccac cgcgagcgtt tcgtcgtcga gcagccgccc
    11281 tactcgatct tcgcccggcg catcgaagcg gacatcctgc ccacctgtca gcgttacggc
    11341 atgggggttg ttgcgtggag cccgttgtcg caaggctggc tcacgggcaa gtggcgccgc
    11401 gacgcgccgc ccaacgacac caaccggcag aagctgcagc cgcacctgta cgacatggcc
    11461 cgagccgaca atcagcgcaa gctcgacctg gtggagcaac tggcggcggt tgcgagcgaa
    11521 gcgggcatca gcctgatgca catggccatc gccttcgtct gcgcccatcc cgcggtgacc
    11581 tcggcgatca tcggcccgcg cacgcccgag cagcttcagg ggctgctcga tggcgccgac
    11641 gtgaccctcg gcgacgacgt gctcgatgcc atagatgcca tcgtcgctcc ggggactacc
    11701 ctcagccgcg acgacgacgg ctatgtggcc ccggagatcg ccaacaaggc cctgcgccgc
    11761 cggttcgtgg ccgaggaggt tcacgaggcc gcgcgggaaa ccctcaggaa catcgaggcg
    11821 taccgcgaac agcagggaaa gaaatgagca cgaccaccgc tcttgcagcc gtcgcctggg
    11881 cgccggacga cccgtttgcc ttcgaggagg tggagctagg gccgctgggc gcggacgagg
    11941 tgctggtccg gatcgtagcc tcgggcatct gccacaccga cgttgccgtc aaggagcagt
    12001 cggtcaagct gccgttgccc atggttctcg gccacgaagg atccgggatc gtcgaggccg
    12061 ttggcagcg
//
LOCUS       DBNHKV010000413         9795 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_625123, whole genome shotgun sequence.
ACCESSION   DBNHKV010000413 DBNHKV010000000
VERSION     DBNHKV010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9795)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9795
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_625123"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="AC16PP_13450"
     CDS             <1..826
                     /locus_tag="AC16PP_13450"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6482681.1"
                     /translation="GVVFPTIGILWDKEDDPELAMAYARAYNNWQWDFAKPALDRILP
                     IAHVPLYDVDLAYEELKRCLKLGFKGMFMAPEPVCGRRPSHPDFDPIWQLLIDADLPI
                     CIHLIVRFDRVVNLSNTQWWDVTRERPDTVFSFGLGGTMQLIPAVAALVCDGLFERHP
                     RLKVAIVESGAGYVRYLMDRLDEKYARFKATSCLSRPPSEYIRENFWFVMDPSEASID
                     AQCDLVGEDKFLWGSDYPHVDSHLTAMQELHAALEPMSEHRRTLVLGENARRLFRI"
     gene            complement(845..3853)
                     /locus_tag="AC16PP_13455"
     CDS             complement(845..3853)
                     /locus_tag="AC16PP_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482682.1"
                     /translation="MGRNGEGRAPRGTRGFQAGCLSALLFLGACTGSSEKIFEQSEEL
                     SLRGSVGDGPIVGAQIVVEDAEGQMVFTAESDAAANYQIDVPDGTDLPVTIRVSGGTD
                     LVTGRGADFELLAVAFTSGDLTVNASPYTTLAVKTAQCMGGLSEANLATAWQYIDANM
                     NLGWDGEIIPNPMNEPIADDNIATVLVGNEALGEVIRRTSAALAPTTEALSTDEVIQM
                     LACDIADGQLDGVGPDVSPRVALTLLGTATAVRVEVISGALFVDGQDATPLLDSALQT
                     VLPGSLRSVTELAPNQQLIDQTLSGLAVLGTFDFAEATTLAIALEGATPDTARGQLAP
                     ALTSSVTTNLQDLGSQLALADTTTIEELLLRQADSAGAPVPVVSFDAEPSSVEAGTST
                     RLSWAASDADECLASGGWSGSQATSGTFQTPLLEQSTEYVLSCVGLGGTGTGRVFVEV
                     TQPAPVPATDAGSDPDPASDPDPVSDPISDPDPISDPDPISDPDPVSDPEPEPEPEPA
                     PAPAPPPAPPVVSISADRTTVDAGESALLTWNATDSESCSASGNWSGSRSVSGSLSIG
                     PLSASGTYALTCTGPGGSDTDSVSITVIEPAPRPGVVLTAASTSVEQGSATQLDWTST
                     DATSCSASGAWSGDRAISGSASTGVLSDDSTFTLTCSGPGGSASESVTVVVTEPPAPT
                     VNLTATSTEIDSGDSTTLTWSSSDATSCTASGSWSGDRGTSGDASVSPSATSSYTLTC
                     TGAGGATSASTTITVDPAPVPAPLVSLSVTPGTIDSGDTAVLSWTSSDTTSCTASGGW
                     EGTRDASGSVDVAPTATTTYTLTCSGDGGSDTATVTLSVVSAAPTVTFSASETLVDPG
                     GLVTVSWSSTDATGCTASGGWSGARDTSGSEVVGPVDSATTYTLTCTGPGGNAVEMLT
                     VETLNAVTLEWAAPAENVDGTPLTDLAGYRIYWGQTSRNYTDVVELNDPYTTSHTLNL
                     ASGDYHVAMTALDQDGNESGYSNEVVKTAQ"
     gene            complement(4229..5068)
                     /locus_tag="AC16PP_13460"
     CDS             complement(4229..5068)
                     /locus_tag="AC16PP_13460"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016968880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6482683.1"
                     /translation="MTDWLKAGNNAVITGGASGIGLAAATHYLSAGMNVLIADLDEDA
                     LSTAAASLADSGSGTLHTQVCDVSDFAQVETLQATAAAKLGNVHCLMNNAGAGFPPSA
                     PWENLDVWKKQVEINLWGIIHGCQAFVPAMLEAGERGAIVNTGSKQGITNPPGGYAYN
                     LSKAGVKNYTESLAHALRQVEGCQLSAHLLVPGFTYTGMISRFIPEKPAGAWTPDEVV
                     AFLIRSLDRGDFYVLCPDNDTPRDLDEKRIQWNTDDLIRNRSALSRWDPKYKDAYEAF
                     VSD"
     gene            complement(5065..5715)
                     /locus_tag="AC16PP_13465"
     CDS             complement(5065..5715)
                     /locus_tag="AC16PP_13465"
                     /inference="COORDINATES: protein motif:HMM:NF016240.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HIR6482684.1"
                     /translation="MNEARVLGCLMEKSVITPDQYPLTVNALTNACNQKSSRDPVLSL
                     EPGVVQRTARDLEAKHLIASDENFKSRVEKFSQRFCNTPFADVQLDSAEFAVMCLLLL
                     RGPQTPGELRSRSGRLHDFADNQAVVETLQGLLEREGEPLVARLPRKPNRQDHEYAHL
                     LSGTIESMPVAEAAAHVRAPGRTAELEGRVAALEQEVRELREALQQMRAEREEEKS"
     gene            5879..6712
                     /locus_tag="AC16PP_13470"
     CDS             5879..6712
                     /locus_tag="AC16PP_13470"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6482685.1"
                     /translation="MNNFEGKVAVITGGATGIGFSFAKRFGAAGAKVIIAGRRQQKLD
                     ESVAALQQLGYEAAGFVCDVSDLTQVEALADFAEAKFGGADVIMNNAGILIPPSTVID
                     ADPKLVRQLLDVNYMGVWNGASVFGKRFIERGTPAAIYNVGSENAFFNIVPGMFAYES
                     AKHGLHSMTDALREETPDFIDVSFICPGFVQSEMTAMAGDIPMDVEEYTSIAWPQILN
                     GEYYVVSHPYNVVWIQHRYDEMMKAYETYAPRYDGDWQYDPRAMAAKAAEAAKDDWRK
                     R"
     gene            6782..7036
                     /locus_tag="AC16PP_13475"
     CDS             6782..7036
                     /locus_tag="AC16PP_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482686.1"
                     /translation="MTEQPLIGPDDPPPFTVFNPEGSARVLFVSDHGGRAFPKAMEQL
                     GLADWVLERHVAWDIGSGDVAERLARQFDSPGRWSGRRGI"
     gene            complement(7016..7447)
                     /locus_tag="AC16PP_13480"
     CDS             complement(7016..7447)
                     /locus_tag="AC16PP_13480"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6482687.1"
                     /translation="MGIPIRVLIVLLSALAIAGCQTAAPEGERSGYEYDGRNLYLGYC
                     ASCHGLSGAGDGPVAAGMAFKMEDLRTLEARNGTFPADGLREVIDGRTLRSVHGTNDM
                     PVWGWQFLLAEDSEEHVAARIDALVEYLRSIQHGDSDPAAP"
     gene            complement(7661..9211)
                     /locus_tag="AC16PP_13485"
     CDS             complement(7661..9211)
                     /locus_tag="AC16PP_13485"
                     /inference="COORDINATES: protein motif:HMM:NF012686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR6482688.1"
                     /translation="MEISDQSQLALIALCIYFVVVVGLGLFYSRKSQSTEDFILAGHS
                     LTTPFVMGSVVATWMGGAVILGGAKEAFVGGFQAIVWDPWSPMLTLILCGFFFVSVFR
                     RSRFTTSIDFYNARYNRGIGLAALSISMVAYISWISAQLLSLGVIIKVVTGMDAVMAT
                     LAGSMIVLVVSLSGGLWALSRSDMLAFIILSVVLILVLPLALDAVGGPAAFIENAGTR
                     DGSPPFSLFYTEAPTAAGEPTGFYGYLGMLGVFYMLAAWFSVAIGDLGGSVLTARALA
                     AKDEVSATRGFLFGGVIYLLLGMIPVIVGMCVFILSPDIAEADLDDVLPIFVQQYLPD
                     WITVLFFVAVASAIVSTAGDTILTGGALLGYTALGAVKPESSDRTRLRATRTAMVLFT
                     AIALVFGLAMGDLYRLLVFAGAVAFPVTSAAFVCGVLWKRANVPGAWASIAAGTVSWV
                     IFVVLLLPEVDWEVWDALYIGAVPAFLCSAIAIVVVSLATRRTHPPNPVRDVDGNDIS
                     GGPLFRWR"
     gene            complement(9354..>9795)
                     /locus_tag="AC16PP_13490"
     CDS             complement(9354..>9795)
                     /locus_tag="AC16PP_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009681892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR6482689.1"
                     /translation="AILRELQNDARLPNTALAERVGLSDSACLRRVRRLEQSGVIRGY
                     VALVDETLAGYPDSVFVQITLVSQQRKDLEAFEDAVARVPEVMECYLMSGASDYILRV
                     VVADARDYERIHSQQLTRLPGVDRVHSNFALRTVIENTEIPIRT"
BASE COUNT         1797 a         3089 c         3116 g         1793 t
ORIGIN      
        1 gggcgtggtg ttccccacca taggaattct gtgggacaag gaagacgatc ccgagctggc
       61 aatggcgtac gcgcgcgcct acaacaactg gcagtgggat tttgccaaac cagctttgga
      121 tcgcatcctg ccgatcgctc atgttcccct gtacgacgtg gatcttgcct acgaggaact
      181 caagcgctgc ctgaagctcg gcttcaaggg catgtttatg gcgccagagc ccgtgtgtgg
      241 caggcgccct tcccacccgg atttcgatcc catatggcag ttgctgatcg atgccgacct
      301 gcccatatgc attcacctca tcgtacgttt cgacagggtg gtgaatcttt cgaataccca
      361 gtggtgggac gtaacccggg aacgtccgga tacggtgttc tcgttcgggc tgggcggaac
      421 catgcagctg ataccggcgg tcgccgcgct cgtgtgcgac ggcttgttcg agcgccaccc
      481 ccggttgaag gtggccatcg tcgagtccgg cgccggatac gtccgctact tgatggaccg
      541 attggacgag aaatacgcta ggttcaaggc aacgagttgc ctgtccaggc cacccagcga
      601 atacatccgg gaaaacttct ggttcgtcat ggacccgagc gaagccagca tcgacgctca
      661 gtgcgatctg gtaggggagg acaagttcct ttgggggtct gactatcccc atgtggattc
      721 ccacctcacc gccatgcagg agctccatgc cgccctcgaa cccatgtccg agcatcgccg
      781 caccctggtg ctcggtgaaa acgcgcgcag gctgttccgt atctgaccag agacagcgaa
      841 gtcgctactg ggcggtcttc accacctcgt tcgagtagcc gctttcgttg ccgtcctggt
      901 ccagcgccgt catggcgacg tggtaatcgc cggaggccag attcagggta tggctggtgg
      961 tatacggatc attgagctcg accacgtccg tgtagttgcg actggtctgc ccccagtaga
     1021 tccgataccc ggccagatcg gtcaatgggg taccgtcgac gttctcagcg ggcgctgccc
     1081 attccagagt gaccgcattg agcgtctcaa cagtgagcat ctccaccgca ttgcctcctg
     1141 ggcccgtgca ggtgagggtg tatgtggttg ccgagtcgac tggcccaacc acctcggagc
     1201 cactggtgtc gcgggcaccg ctccagccgc cggaagcggt acagcccgtg gcatccgtcg
     1261 atgaccagct gactgtgacg aggccgcctg gatcgaccag tgtttctgag gcgctgaacg
     1321 tgaccgtcgg cgccgcgctc accaccgaca gggtcacggt ggcggtatcg ctgccgccat
     1381 caccgctgca ggtaagggtg taggtcgtgg tggccgtggg cgccacatcg acgctgccgc
     1441 tggcatcgcg ggtgccttcc cagccaccac tggcggtgca gcttgtggtg tcgctgctgg
     1501 tccagctcag aacggccgtg tcgccgctgt cgatggtgcc gggagtaacg gacagggaaa
     1561 ccagcggcgc aggtactggg gccgggtcga cggtgatggt cgtgctggca ctggttgccc
     1621 cacccgctcc ggtgcaggtc agcgtgtagc tgctcgttgc gctcggcgac acgctggcat
     1681 cgccgctggt gccgcgatcg ccgctccagc taccgctggc ggtacagctg gtggcgtcgc
     1741 tgctcgacca ggtcagagtc gtgctgtcac cgctgtcgat ttccgtactg gtcgcggtca
     1801 ggttcacagt gggagcaggt ggttcggtaa caacgactgt cacgctttcc gaagcgctac
     1861 cgccgggtcc tgagcaggtc agggtaaacg tgctgtcatc gctcagcacg ccggtagaag
     1921 cggagccgga gatagcccga tctccgctcc aggcgccgct ggcgctgcag ctcgttgcat
     1981 ccgtgctggt ccagtcgagc tgggtcgccg atccctgctc aacgctggtg gatgcggctg
     2041 tcagaaccac cccgggtcgg ggagcaggct cgatgaccgt gatcgaaacg ctgtctgtgt
     2101 ccgatccgcc gggaccggta caggtcagag cataggttcc gctggcggac aggggcccaa
     2161 tgctgagact gccgctgacg gagcgggatc ctgaccagtt cccggaggcg ctgcagctct
     2221 ctgagtctgt tgcgttccag gtcagcagcg cgctctcgcc tgcatcgacc gtcgtacgat
     2281 cggcgctgat ggagacgacg ggcggcgcag gaggaggcgc cggagccggt gcgggttccg
     2341 gttccggttc cggttccgga tctgagaccg ggtccgggtc cgagatcgga tcagggtccg
     2401 agatcgggtc cggatctgag atcggatcgg agaccgggtc cgggtctgaa gccgggtccg
     2461 ggtcggagcc cgcgtccgtt gccgggaccg gtgcgggttg agtcacttct acaaacactc
     2521 gcccggtgcc cgtgcccccc aggcccacgc agctcaggac atattccgtg ctctgctcca
     2581 gcagcggggt ctgaaaagtg ccactggtgg cctgcgagcc tgaccatcca cccgaggcca
     2641 ggcactcgtc ggcgtcgctg gccgcccagc tcaggcgggt gctggttccc gcctcgacag
     2701 acgatggctc cgcatcaaac gaaacgactg gaaccggcgc accggcgctg tcggcttggc
     2761 gcagcaacag ctcctctatg gttgtcgtat ccgccagagc caactgactg cccaggtcct
     2821 ggagattggt ggtcacggag ctggtgagag ccggcgccag ctggccgcgg gcggtatccg
     2881 gggttgcacc ttcgagggcg atcgcaagag tcgtggcttc ggcaaagtcg aacgtcccaa
     2941 gcaccgccag gccgctcagg gtctggtcga tgagctgctg attgggcgcc agctcagtga
     3001 cggagcggag actgcccggc agcacggtct gcagcgcgct gtccaggagc ggggtggcgt
     3061 cctgtccatc gacgaacagg gcaccgctga tgacttccac cctgaccgcg gttgccgtgc
     3121 cgagcagcgt gagcgcgacc cggggagaca catctggtcc gacgccgtcc agctggccgt
     3181 cagcaatgtc gcaggcaagc atctggatca cttcgtccgt ggagagcgct tccgtggtcg
     3241 gcgccagcgc ggcgctcgtg cggcggatca cctcaccgag ggcttcgtta cccaccagca
     3301 cggtggcgat gttgtcatcg gcaatcggct cgttcatggg attcgggatg atctccccat
     3361 cccagccgag gttcatgttg gcatcgatgt attgccaggc ggtagcgaga ttggcctcgc
     3421 tgagtccacc catgcactgg gcggtcttca ctgccagggt cgtgtagggt gaggcgttga
     3481 cggtgagatc gccagacgtg aacgccacag ccagcagttc aaaatccgcg ccgcggccgg
     3541 tgaccagatc ggtgccgccg ctcacccgaa tggtcacagg cagatcggtg ccgtcgggaa
     3601 catcgatctg atagttggcg gcggcatcgc tctcggcggt gaacaccatt tggccttcgg
     3661 cgtcctccac cacgatctgc gcgccgacga tggggccgtc cccaacgctg ccgcgaagac
     3721 tcagctcttc actttgctcg aaaatctttt cgctgctgcc ggtgcatgcg cccagaaaaa
     3781 gtagcgcact caggcagcct gcctgaaacc cccgcgttcc cctgggggcc cttccctctc
     3841 cgtttcgtcc cactttgcct ctccgctctt aaagccaaca tctgcgcggc ggatcggtgg
     3901 gtgccatctg cggcgcatag cgtcagaacg gctcgagtat cggaaaaggg gacgtgtctt
     3961 cccgtgaggc ggtcgacact ttcgaccctg atgcgagcgt tttgtggaag gatcgtggtt
     4021 ggtttgagaa aacgtgacgg gtgcacaatg gttgaggttc ttgggtttcg cccgccccgt
     4081 aagttgccac ctcgaaaacg agcagcgcgt tgcccgacat cttgccggta aatccgtagc
     4141 cggagctgac gatgagctgg ccctggtagg cgatcggagc ggcgccgcga ggtgcgctca
     4201 accgccagcc acggaactcc aggcattgtc agtcggagac gaaggcctca taggcgtcct
     4261 tgtacttcgg atcccagcgg gacagggcgg atcggttcct gatcaggtcg tccgtgttcc
     4321 actggatgcg cttctcgtcg aggtcccgcg gcgtgtcgtt gtccgggcag agcacgtaga
     4381 agtcgcctcg atcgagcgat cgaatcagaa atgccaccac ctcgtcgggc gtccaggcgc
     4441 cggccggctt ctcgggaatg aagcgggaga tcatgccggt gtaggtgaag ccgggcacca
     4501 gcaggtgggc ggagagctgg cagccttcca cctgccggag cgcgtgggcc aggctctcgg
     4561 tgtagttctt cacgcccgcc ttggacaggt tgtaggcgta gccgcccggc gggttggtga
     4621 tgccctgctt cgagccggta ttgacgatcg ctccccgctc gccggcttcc agcatggcag
     4681 ggacgaaggc ctggcagccg tgaatgatgc cccagaggtt gatctccacc tgcttcttcc
     4741 agacgtccag gttctcccag ggcgcgctgg gcgggaaccc cgcacctgcg ttgttcatca
     4801 ggcagtgcac gttgccgagc tttgccgcgg ctgtggcctg cagcgtctcc acctgcgcaa
     4861 agtcggagac gtcgcagacc tgagtgtgca gggtgccgct gccactgtca gccagggacg
     4921 ctgccgctgt gctcagagcg tcctcgtcga gatcggcgat cagcacgttc atgccggccg
     4981 aaagatagtg ggtggctgcc gccaggccga tgccactggc gccgccggtg atcaccgcgt
     5041 tgttgcctgc ttttagccag tctgtcatga cttctcttcc tcgcgctctg cgcgcatttg
     5101 ttgcagcgct tcgcgcagct cgcgcacctc ctgctccaac gcggccaccc gaccctcgag
     5161 ctcggccgta cggccggggg cgcgcacgtg ggccgccgct tccgcaaccg gcatcgactc
     5221 gatggtgccg gacagcaggt gcgcatactc gtggtcctgc cgattcggtt tgcgcggcag
     5281 ccgtgccacc agcggctcac cttcacgttc cagcaggcct tggagcgttt ccaccaccgc
     5341 ctggttgtcg gcgaagtcat gcaagcgtcc gctgcgggac cgcaattcgc ccggcgtctg
     5401 gggaccccgc agcaaaagca ggcacatcac ggcgaattcc gcgctgtcca actgcacatc
     5461 ggcaaacggc gtgttgcaga aacgctggct gaatttctcc acccggctct tgaaattctc
     5521 gtcgctggcg atcaagtgct tggcttcgag atcacgagcc gttcgctgaa cgacgcccgg
     5581 ctcgagagag agcacgggat cgcgactgga tttctggttg cacgcattgg tcagcgcgtt
     5641 caccgtcagg gggtattgat ccggcgtgat gaccgatttt tccatcaggc aaccgagcac
     5701 gcgcgcttcg ttcacggtga gttcgatgtt cagcttgcga tctccagact tcgaggtgag
     5761 gcaagtgagt tagggtacca ccgttgagcc gagtccgagg gctgccccgc actaagcggc
     5821 accagtcaag tctgtatact cgagcgtatg tactcgcaac gactcgaggg agaacggctt
     5881 gaacaacttc gaaggcaaag tcgccgtaat caccgggggc gccaccggca tcggtttctc
     5941 gttcgccaaa aggtttggcg cggctggcgc caaggtgatc attgccggca ggcgccagca
     6001 aaagttggat gagtcggtcg ccgccctgca gcagcttggc tatgaagctg caggcttcgt
     6061 gtgcgacgtc tctgacttga cgcaagtgga agcgctggcc gattttgctg aagccaaatt
     6121 cggcggggcc gacgtcatca tgaacaatgc tggaattctg atcccgccat ccacggtgat
     6181 tgatgccgat ccgaagctgg tgcgccagct gttggacgtg aactatatgg gcgtctggaa
     6241 cggagcatcc gtcttcggca agcgcttcat cgaacggggc acgcccgcgg ctatctacaa
     6301 cgtcgggtcc gagaacgcgt tcttcaacat cgttcccggc atgttcgctt atgagagcgc
     6361 caagcatggc ctgcattcca tgacggatgc gctgcgcgag gaaacgcccg acttcattga
     6421 cgtttccttc atctgcccgg ggttcgtgca gtctgaaatg accgcaatgg cgggcgacat
     6481 ccccatggac gtggaagagt acacgtccat tgcctggccg cagattctaa acggcgagta
     6541 ctacgtggtg tcgcacccct acaacgtggt ttggatccag catcgctacg acgagatgat
     6601 gaaggcatac gagacgtacg cgccccgcta cgacggcgac tggcagtacg acccccgagc
     6661 gatggcagcg aaagccgccg aggcggcgaa ggacgactgg agaaaacggt gagccaactc
     6721 gacctgcttc gcgcccttgc catctgctca cgaagaatca ggagtaggaa aaccattcct
     6781 catgaccgag cagcctctca tcggaccgga cgatccgcct cctttcaccg ttttcaaccc
     6841 ggaaggcagc gctcgcgtgc tcttcgtgag cgaccacggt ggccgggctt ttccaaaggc
     6901 gatggagcag ctggggctgg cggactgggt gctcgagcgg cacgtcgcct gggatatcgg
     6961 ctctggtgac gtggcggagc ggttggcgcg ccaattcgac tcgcctggcc gctggtcagg
     7021 gcgccgcggg atctgagtcg ccgtgctgta tggatcgcag atactcgact agagcgtcga
     7081 tgcgcgccgc tacgtgctcc tccgagtcct ctgcaaggag gaactgccac ccccacaccg
     7141 gcatgtcgtt ggtgccgtgc acagaccgca aggtgcgccc gtcaatgact tctctcaacc
     7201 cgtcggcggg aaacgttccg ttgcgcgcct cgagcgttcg aagatcttcc atcttgaacg
     7261 ccatgcctgc agccaccgga ccgtcgcctg cgccgctcaa cccgtggcag ctcgcgcagt
     7321 aacccagata gaggtttctg ccatcgtact cgtagccaga ccgttcaccc tccggcgcgg
     7381 cagtctggca accggcaatg gcaagagcag acagcagaac gatcagaact cggatcggga
     7441 ttcccatggc atctccacgt attggtctag cctctgatga tgcgggtctg actgggattt
     7501 ggtgattcgg ggttccactt agtggcatcg gctggcgtga cccaccggca tcagaggaac
     7561 cggccaacaa cctgacgagg agataaggat acgcagcggc agctttcgta ggcggtcgcg
     7621 gtggggggac tgccgagccg cgcgcgtttg cgcagcctcg tcatcgccat ctgaacagcg
     7681 gcccaccgga aatgtcgttt ccatccacgt cgcgtaccgg attgggcggg tgggtcctgc
     7741 gcgtggcgag ggacacaacc acgatggcaa tcgcgctgca aaggaaagcg gggaccgcgc
     7801 cgatgtagag ggcatcccag acttcccagt cgacctcggg cagaaggagc acgacgaaga
     7861 tcacccagct gacggtgcca gcagcgatcg acgcccaggc gcccgggacg ttggcccgct
     7921 tccacagcac gccgcatacg aaggccgccg aggtgaccgg gaacgccacc gcgcccgcaa
     7981 acaccagcag ccggtagagg tcgcccatgg cgaggccgaa cacgagcgcg atggccgtga
     8041 acagaaccat cgcggtccgc gttgcgcgca gtctggtccg atcggagctc tccggcttga
     8101 cggcaccgag cgccgtgtag cccagcaggg cgccgccagt caggatggta tccccggccg
     8161 tgctgacgat ggcggacgcc acggcaacga aaaacagcac cgtgatccaa tccggcaggt
     8221 attgctgcac gaagatcgga agcacgtcgt ccagatcggc ttctgcgatg tcgggactca
     8281 ggatgaagac gcacatgccc acgatcaccg gaatcatccc aagcagcagg tagatgaccc
     8341 cgccgaaaag aaagccccgc gttgccgaga cttcgtcctt tgccgccaga gcccgggcgg
     8401 tgagcaccga gccgccgaga tcgccgatgg caaccgagaa ccaggcggcc agcatgtaga
     8461 agacgccgag catgcccagg tagccgtaga agcccgtcgg ttcgccagcc gcggttggcg
     8521 cctcggtgta gaacaagctg aagggcggcg atccatccct ggtgcccgcg ttctcgatga
     8581 aagcagcggg accaccgacc gcatccagcg ccaggggcag caccaggatc agcaccacgg
     8641 aaaggatgat gaaggcgagc atgtcggatc gggacagcgc ccacagcccg ccggacagcg
     8701 aaacgacgag cacgatcatg ctgccagcca gagtcgccat gaccgcgtcc attccggtta
     8761 cgaccttgat gatcacaccg agagagagca gctgcgccga tatccaggag atatacgcca
     8821 ccatgctgat actcagcgcc gcaagcccga tcccacggtt gtaccgggca ttgtagaagt
     8881 cgatggaagt ggtgaagcgg gaacgccgga acacgctgac aaagaagaaa ccacacagaa
     8941 tcagggtcag catgggcgac cacggatccc agacgatggc ctgaaagcct ccgacgaagg
     9001 cttccttggc cccaccgagt atcaccgctc cgcccatcca ggtcgcaacc acggagccca
     9061 tgacgaaggg cgtcgtgagg ctgtgcccgg ccaggatgaa atcttcggtc gattggcttt
     9121 ttcgggaata gaagaggccc agccccacga cgacgacgaa gtagatgcac agggcgatca
     9181 gcgccaattg agattggtca gaaatttcca tcccgactca tccgcctccc cgtcgtcgaa
     9241 gcacgccgcg gccgacttat ccccccggcg aatccagcat caatctgcca ccctcaccga
     9301 ttctgctagt gcgatcttac tctggaccac tagtggaaac ccctaacgct tggctaggtg
     9361 cgaatcggta tctccgtgtt ctcgatgacc gtgcgcagcg cgaagttcga gtgcacccgg
     9421 tccacgcccg gcagccgcgt cagctgctgg ctgtggatac gctcgtagtc ccgggcatct
     9481 gcgaccacga cccgcaggat gtagtccgag gcgcccgaca tcagatagca ctccattacc
     9541 tccggaactc tggccaccgc atcctcgaat gcctccagat ccttccgctg ctgactgacc
     9601 agggtgatct gcacgaacac gctgtccggg taaccggcca gggtctcgtc gaccagcgcc
     9661 acgtagcccc ggatcacgcc cgattgctcg agacgcctca cacggcgcag acacgcggag
     9721 tccgacaacc cgacccgttc cgccagggcg gtgttcggca gccgggcatc gttctgaagc
     9781 tcccgcagaa tcgcg
//
LOCUS       DBNHKV010000414         2231 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1051785, whole genome shotgun sequence.
ACCESSION   DBNHKV010000414 DBNHKV010000000
VERSION     DBNHKV010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1051785"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="AC16PP_13495"
     CDS             complement(<1..452)
                     /locus_tag="AC16PP_13495"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR6482690.1"
                     /translation="MLIEIFSDVVCPWCYIGKRRLDMVMQTEVGEGVELAWRPYQLYP
                     NIPAGGLLREDVLRARHPEADDEDLETLKRKVPSRIRAEAEGVGIEFDFAAMATVPNT
                     RLAHRLLMFVEDVVGSLALQHDLAEELFSLHFCHGQDVGDAEVLVEAAR"
     gene            549..992
                     /locus_tag="AC16PP_13500"
     CDS             549..992
                     /locus_tag="AC16PP_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482691.1"
                     /translation="MSEPTPIRPQPSDADLPALYPLTGVVLATVLGSFIAAVVVVYLN
                     YRSLGSTALARKAVIGGLVVYAGLLGLTVVLPRETYMSIIFIGVQAGLAYVVANALQG
                     DAIAYHVSRGGPIHSLLRAAGVGLLTGLAVLFALLTATALILGTS"
     gene            complement(1034..1801)
                     /locus_tag="AC16PP_13505"
     CDS             complement(1034..1801)
                     /locus_tag="AC16PP_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482692.1"
                     /translation="MSVDQDLDELVLLFTADGGKVVSEIRYGEFEALIKKETSLAEHA
                     ASVVKAAYALVGGGLTLRGIVFFRFTVDEDGKIVGSFNLPLRYLLDNAGLGPDLGAGP
                     IQLACRGQCPVPWHSVNLWQPEGEGDGHPAMLAQKAIWRNRTGLKRTAAPPMAQPLRS
                     QPVEPPVIDEQRHLEARLTAAFGEEGKVNLTQLVQQHSAQLSEVGQQHRLDLEEQHRT
                     FQDQLREARDEIHKLKSALRREQERNRRLQSLLRGDP"
BASE COUNT          427 a          713 c          657 g          434 t
ORIGIN      
        1 cgcgccgctt ccaccagcac ctcggcatcg cccacgtctt gcccgtgaca gaaatgaagc
       61 gagaagagct cctcggccag gtcgtgctgc agcgccaatg agccgacgac gtcctcgacg
      121 aacatcagca gcctgtgggc caggcgcgtg ttcggaacgg ttgccatcgc ggcaaagtcg
      181 aattcgattc cgacgccttc cgcctctgca cgaatacggg agggaacttt gcgcttgagc
      241 gtctcgagat cctcgtcatc cgcctccgga tgacgggctc tgagcacgtc ttcgcgcagc
      301 agcccgccag cggggatgtt cggatagagc tgatagggcc gccatgcaag ctcgacgccc
      361 tcaccgacct cggtttgcat gaccatgtcc aatcgtcgct tgccgatata gcaccaggga
      421 cagaccacgt cggagaagat ttcgatcagc attgccggcc tcgcatgcac cggtaggtga
      481 tcgattcatt gttagggtgg cgggcccgtt cctggtagaa cagacggtca gactttaaac
      541 tccgcagcat gtccgaaccc acgcccatcc ggccgcagcc ttctgatgcc gaccttccgg
      601 cgctctaccc gctcaccggc gtggtgcttg ccacagtcct gggttcgttc atcgccgccg
      661 tcgtggtcgt ctacctgaac taccggtcgc tgggttcaac cgctctcgca cgcaaggcgg
      721 tgatcggtgg gctggtggtc tacgcgggtc tactgggact caccgtggtg ctgccgagag
      781 aaacctacat gagcatcatc ttcatcggcg tgcaggcggg gctggcatac gttgtcgcca
      841 acgccctgca gggagacgcc attgcctacc acgtttcacg gggcggaccc atccattcgt
      901 tactgcgcgc cgccggcgtc gggcttctga cagggcttgc ggtactgttc gctctgctga
      961 cggcgaccgc gctgatcctg ggaacgtctt agggccgctg aaaagcagca ggcggcagat
     1021 ggcccgaacc gggtcaggga tccccgcgca gcagcgactg aagacggcga ttgcgttcct
     1081 gctcacgcct gagtgctgat ttgagcttgt ggatctcgtc acgggcctcg cgaagctgat
     1141 cctgaaaggt ccgatgctgc tcttcgagat ccaaccgatg ctgctggcca acttccgaaa
     1201 gctgggcgct gtgctgctga accagctggg tgagattgac cttgccttcc tcaccgaagg
     1261 cggcggtcag ccgagcttcg agatgccgtt gctcatcgat gaccgggggt tccacaggct
     1321 ggcttcgcag cggttgggcc atggggggcg ctgccgtacg tttcagaccg gtccggtttc
     1381 tccagatcgc cttctgggcg agcatcgccg gatggccgtc tccttccccc tccggctgcc
     1441 agagattgac ggagtgccat ggaaccggac actgcccccg acaggccagt tgaatcggcc
     1501 ctgctccgag atcgggaccg agcccggcgt tgtccaggag ataacgcaac ggcaggttga
     1561 aagaccccac gatcttgccg tcctcatcga cggtgaagcg aaagaagacg ataccgcgca
     1621 acgtcagacc cccgcctacc agggcgtagg cagccttcac cacgctggcc gcatgctcgg
     1681 ccaggctcgt ctcttttttg atgagagcct cgaattctcc atagcggatc tcggagacga
     1741 ctttgccgcc gtctgccgtg aacaggagaa cgagttcatc caaatcctga tctactgaca
     1801 tttccaagct tgtcaacgcg cccggttgga acagcccatc cctatcgaaa gtggccatcc
     1861 gcagaccaga agcactcttc catgaagctc gcacgggaca caaacaccgc gactcgcctc
     1921 attacagcag atggccattc cccatactgc caatgggatc acggctttcc gctgaattcg
     1981 gcgggcgacg agcgcagcga atatgcgggt ggcggcgaag aaacagacgg ctacttgact
     2041 agagctcgta ggcatccacc cagaccgatt cgccgtcgtc agccgacgac tcgtcgatcc
     2101 agtgattctc gagctcgggc tcgccgatct cttcaagttc catgtgacaa agacctccag
     2161 gaacccagat cctagaccga gtcgatgaac ctaacgtgaa acgatcgcgg ctggcgggct
     2221 cagctttgag c
//
LOCUS       DBNHKV010000415         4875 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_343819, whole genome shotgun sequence.
ACCESSION   DBNHKV010000415 DBNHKV010000000
VERSION     DBNHKV010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_343819"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="AC16PP_13510"
     CDS             complement(<1..278)
                     /locus_tag="AC16PP_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482693.1"
                     /translation="MRPLTSWRIVQALGKQRYLCLSLLAFLFALPLAGDEPGARLTTQ
                     LVLVFVFITGPLAVARTRATLWITGVFAIVVFVMGIGSVLGGGDWFLGT"
     gene            complement(394..1314)
                     /locus_tag="AC16PP_13515"
     CDS             complement(394..1314)
                     /locus_tag="AC16PP_13515"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR6482694.1"
                     /translation="MAKHGIVADFELIPDYMVSARLLHGYIIEAVSHGPPGAVSVAAG
                     LFNAAFQPNPFSDTAWKSVTLLEAIRRHNANVTRYSPENRFRFSVAKGEGHWEKQDRS
                     PLAETEIFCVANLIGHVRMSLGDGWSPPSVDVSVAEPSTLASLPLFEGVSVRAVPISA
                     TIHIPLSALALPSSSRDAGLSERALPDDFGDLDFVQSMRELIRSYVRVGELSFSNVAL
                     AAGLSRRTLQRRLSDVGISYSGMVDQVRFEVARELLLESPELPVTQIGYELGYADPGS
                     FTRAFRRFAGTSPIAFRRASPSGMPTAEVA"
     gene            1510..1782
                     /locus_tag="AC16PP_13520"
     CDS             1510..1782
                     /locus_tag="AC16PP_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482695.1"
                     /translation="MSNQIRDADAIRPALFDIEGAIAGYFEWKKQLYDRRGFATSTTL
                     LALYQHADRSISGNKHGAGLIFRWQGSWDLVGRERRRRVSERSALS"
     gene            1955..2278
                     /locus_tag="AC16PP_13525"
     CDS             1955..2278
                     /locus_tag="AC16PP_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482696.1"
                     /translation="MGKGSTERRRDAVRDEWVIESSYKLQLTRTVTLLPNVRVLIGPA
                     ADPGADTTVVLGIRAGIAIWRSPQHPARTQQRTAANSITANVMVAARRRWHEVASGRR
                     IANLG"
     gene            2334..3713
                     /locus_tag="AC16PP_13530"
     CDS             2334..3713
                     /locus_tag="AC16PP_13530"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase family protein"
                     /protein_id="HIR6482697.1"
                     /translation="MNILGMLLTLILLMTWSSPSRAEDEPARVLFTNVNVFDGYAKDL
                     QRGMHVLVEGNLIKTISDQPIAAADAQVIDGGGRTLLPGFIENHAHLMLMGPSLPAME
                     ANTTWEDFAIHGTRMAEMYLMQGFTTVRDAGGANAGLRRAIDSGQIVGPRFYPSAAFL
                     GTRGGHADFANYTSPVGESTNFGRLNLAQEVDDVADVQKFGRNNFRMGATQLKYMQSG
                     GVVSAFDPWQLLAGSQEEIKAAVQVANEYGSYVMAHSYRKEAIMNALNAGVKSIEHGF
                     AFDCEIAKLMKKKSAYITTNLTAFDPGLLEIPAVASVPSSLAKAKSATATFAGYIDNM
                     KKCPVKRGFQTDCVGSVAACNIQIAYEKHLNNEFFGPHTSLVAMTSVGGEIAALSGDF
                     MNPYPDAKLGIIEEGAYADILIIDGNPFEDFSVVGTGDKWFDAEPRADSPVTIRIIMK
                     DGKLYKNTL"
     gene            3813..>4875
                     /locus_tag="AC16PP_13535"
     CDS             3813..>4875
                     /locus_tag="AC16PP_13535"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR6482698.1"
                     /translation="MNTALTRSRLTGTLCLVLIAGGCASVPERNPVPPSLADAAEVPG
                     MPGIRYWADDAPPYAKAWLERSQEDLLSRYPASFGNPHNYLAISGGGANGAFGAGLLN
                     GWTDSGTRPEFTVVTGISTGALIAPFAFLGPAYDHVLKRVYTEFSTEDLLESRGLLQA
                     LTGEAATDSAPLRARIAEFVDEEVMAAIADEYQRGRSLLITTTNLDAGRSVTWLIGRI
                     AASGSPNALELIRDVMLASASIPAALPPVLVEVEAAGQAYDELHVDGNTSSVVNLYPL
                     ALDWEAVLARLEVDAAPNLYIIRNGHIDITHESVDRQTIAIAGRSIQTLMNYLARGDL
                     YELYLLTQRDGVRYHLANIP"
BASE COUNT         1021 a         1549 c         1476 g          829 t
ORIGIN      
        1 gtgcccagga accaatcgcc tccgcccagg acggaaccaa tccccatgac aaacacgacg
       61 atggcgaaca cgcccgtgat ccagagggtg gcgcgggtgc gggcgacggc cagcggcccg
      121 gtaatgaaca cgaacaccag cacgagctgc gtagtcaggc gggcgccggg ttcgtccccg
      181 gcgagtggca gcgcgaacag gaacgcgagc agcgacaggc agaggtatct ctgcttgcca
      241 agggcctgaa cgattcgcca ggacgtaagt ggccgcatct cggtcatcgt ccgagccagc
      301 ccgctccgct caaagggaag ctcaagattc cttcaatcac gccagcaatc acgccagcaa
      361 tcacgccagc gtctcgagct tgccccgtgc gcctcaagcc acctccgcgg tcggcatgcc
      421 cgacggagaa gcgcgccgaa aggctatggg cgaggtgccg gcgaaacgac gaaatgcgcg
      481 ggtaaacgat cctggatctg cgtaacccag ctcgtagcca atctgagtga cgggtagctc
      541 cggcgactct aggagcaatt cgcgcgcaac ctcgaatcta acctggtcga ccatgccgga
      601 ataggagatg cccacatcgg agaggcgtcg ctggagcgtt cgccgactca agcctgcagc
      661 caaggcaacg ttgctgaaac tcagctcgcc cacgcgcacg taactcctga tcagttcccg
      721 catggattga acgaagtcca gatcaccaaa gtcatccggc agagcccgct cgctcaaacc
      781 ggcatcccgg gaactgctgg gtaatgccag cgcagaaagg gggatgtgaa tagttgcaga
      841 aatcggcacc gcgcgaacgg acacgccttc gaacagcggc aaggatgcca gggtcgatgg
      901 ctccgccacc gatacgtcca cggacggggg cgaccacccg tcccccaagc tcattcgtac
      961 gtgaccgatg aggttggcga cacagaaaat ctcggtctcc gcaagcggcg atctgtcctg
     1021 cttttcccaa tggccctcgc cctttgcgac cgaaaaccga aagcggttct ccgggctgta
     1081 gcgcgtcacg ttggcgttgt gcctgcgaat ggcctcaagc agggtgaccg atttccaggc
     1141 cgtgtcagag aaggggttgg gctgaaaggc agcgttgaac aaccctgcgg cgacgctcac
     1201 ggcaccgggc ggtccatgac tcaccgcttc gatgatgtac ccgtgcagca ggcgcgccga
     1261 gaccatgtag tcgggaatca gctcgaaatc cgccacgatc ccgtgtttcg ccaacagggg
     1321 ctccggcgcc acgccatgct cccgtaacgc atgcatgtaa gcgatgagat acgtgttcct
     1381 gagtagccag attggttgca cttggtagga ccttgagtac cgctcgcgcc gcccggtacc
     1441 atcgcgaaag tggcaccaaa tggcaagtgg gggccgccag gatcgtctac cgacggcggc
     1501 cttgaatcgg tgagcaacca gattcgcgac gctgatgcaa ttcgcccggc cctgttcgac
     1561 atcgaaggcg cgatcgcggg ctatttcgaa tggaaaaagc agctctacga ccgtcgggga
     1621 ttcgccacga gcaccactct tttggccctg tatcagcatg cagacaggtc catctccggc
     1681 aacaagcatg gggccggcct gatattccgc tggcagggaa gctgggacct ggtcggccgg
     1741 gaacgcagac gacgggtgtc ggagcgatcg gcgctgtcat gaagggcatg gtcaccgatg
     1801 agatctggct gggcgaaggg gtttacgacg ccaacgcgtc cagcggcgag ttcgactgga
     1861 gctccgtcgg tgagggcgag tacctcaagc acctcgaggt cggctgcaca ccggccttcg
     1921 cccgtcgagc caagaacaca gtccagctca cgtggtggga aaaggatcga cggaaagacg
     1981 ccgggacgcc gtgcgtgacg aatgggtcat cgagtccagc tacaaacttc agctcacccg
     2041 taccgtgacg ctgctaccca acgtgcgggt cctgatcggc cccgcagccg accccggcgc
     2101 agacacgacc gtggtgctcg ggataagggc aggcatagcc atctggcgca gcccacagca
     2161 tccagcccga acacagcaac gcacagccgc caactccatc acggcaaacg tcatggtcgc
     2221 tgcgcgtcga cggtggcacg aagtggcaag cgggcgccgg atcgccaacc taggatgacc
     2281 gtttgcattg caaactccat ctccaaaaca tggcaccacc caaggaggac aacgtgaata
     2341 ttctgggcat gcttctaacc ctcattttgc tgatgacctg gtcgtcgcca tcccgggccg
     2401 aggatgagcc ggcacgcgtt ctcttcacca acgtgaacgt ttttgacggt tatgcaaagg
     2461 acctgcagcg cggaatgcac gtgctcgtag agggcaacct aatcaaaacg atctcggacc
     2521 aacctatcgc cgccgcggac gcccaggtga tcgatggcgg cggtcggacc ctgctaccgg
     2581 gtttcatcga aaaccatgcc cacctgatgt tgatgggtcc gagcctgccg gctatggaag
     2641 ccaatacgac gtgggaggac tttgccatcc acggtacgcg catggccgaa atgtacctga
     2701 tgcagggctt caccacggtg cgtgatgccg gtggcgcgaa cgccggcctg cgccgtgcga
     2761 tcgattcagg ccagatcgtc gggccgcgct tctacccctc cgcagcgttt ctcggtaccc
     2821 ggggcggtca tgcggatttc gcgaactaca cctcaccggt cggcgaatcc accaacttcg
     2881 gtcgcctgaa tctggcgcag gaggtcgacg acgttgcgga cgtccagaag ttcggccgca
     2941 acaatttccg gatgggcgcc acgcagttga agtacatgca gtccggtggc gtggtttcgg
     3001 ccttcgaccc ctggcagttg cttgccggat cacaggaaga gatcaaggca gccgtgcagg
     3061 tcgccaatga atacggtagc tacgtgatgg cgcattccta caggaaggaa gcgatcatga
     3121 acgcgctgaa tgcaggcgtg aaatcaatcg agcacggctt cgcgttcgac tgcgagatcg
     3181 ccaaactgat gaagaagaaa agcgcctaca tcaccaccaa cctgacggcg ttcgatcccg
     3241 gtcttctcga aatcccggcg gtcgcgagtg tcccgtcgag cttggccaag gcgaaatcag
     3301 cgacggcaac gttcgctggc tatatcgaca acatgaagaa gtgtcccgtc aaacgcggct
     3361 tccagaccga ttgcgtcgga tcagtcgccg cgtgcaacat ccagatcgcc tacgagaagc
     3421 acctgaacaa cgagttcttc ggaccccata cctccttggt ggcgatgacg tccgtcggtg
     3481 gcgagatcgc cgctttgagc ggcgatttca tgaaccccta ccccgacgcc aaactcggca
     3541 tcatcgagga aggtgcctat gccgacatcc tgattatcga cggcaacccg tttgaggatt
     3601 tctcggtcgt cggtacgggc gacaagtggt ttgatgcgga gccgcgggcc gatagcccag
     3661 ttaccatccg catcatcatg aaggacggga aactctacaa gaacactctc tagccaactt
     3721 acgagggctg tcaggccgac caggaagctc gtccagcggg ccggaccagt cggcgtggca
     3781 cgcccggaga tctcgaggtc gcgagccccc ggatgaacac cgcgctgacc aggtcccgcc
     3841 tgaccggaac cctctgcctt gtgctgatcg ccggcgggtg cgcctcggta ccggaacgca
     3901 acccggttcc tccctcgctg gcagacgctg ccgaggttcc cgggatgccc ggcatccgat
     3961 actgggcaga tgatgcgccg ccctacgcga aggcttggct ggagcggtcc caggaagatc
     4021 tgctctcgcg ttaccccgcg agtttcggca acccccacaa ctacctggcc atctcgggcg
     4081 ggggagccaa cggcgcgttt ggagccgggt tgctcaacgg gtggacggac tcaggcaccc
     4141 gcccggagtt cacggtggtc accggtatca gcaccggggc gctgatcgcg ccgttcgcct
     4201 ttctcggccc ggcgtacgat cacgtcttga agcgggtcta caccgaattc tccaccgagg
     4261 acctcctcga gtcccggggc ctgctgcaag cgctgaccgg tgaggcagcc accgattccg
     4321 ctccgctgcg agcccgaata gctgagttcg ttgacgaaga ggtgatggcc gccattgccg
     4381 acgagtacca gcggggcagg tcgctgctca tcaccaccac caatctcgac gccggacgat
     4441 cggtgacctg gctgattggc cggatcgccg cctcgggctc acccaacgcg ctagagctca
     4501 tccgcgacgt catgctggca tcggcatcga tccctgccgc gctcccaccg gtgctcgtgg
     4561 aggtggaggc cgcgggccag gcctatgatg agctgcacgt cgacggcaac accagctccg
     4621 tggtcaacct ttaccccctg gcactggact gggaggcggt gctggcaagg ctagaagtcg
     4681 acgcagcgcc caatctctac atcattcgca acggccatat cgacatcacc catgaaagcg
     4741 tcgaccgaca gaccatcgcc atcgcgggaa ggtcaattca gaccctcatg aactatcttg
     4801 cccgaggcga tctctacgag ctgtatctcc tgacccagcg ggacggcgtt cgatatcatc
     4861 tggccaacat accgg
//
LOCUS       DBNHKV010000416         5583 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_411516, whole genome shotgun sequence.
ACCESSION   DBNHKV010000416 DBNHKV010000000
VERSION     DBNHKV010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_411516"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..656
                     /locus_tag="AC16PP_13540"
     CDS             216..656
                     /locus_tag="AC16PP_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482699.1"
                     /translation="MARLALTGAIIILLSGCAEPFIVFAGGALSGDEKVPPADWSFLE
                     AIDTVQIETDPNDPYSVNVWSVGIGADVYVATGEDGTNWTERIDGDPSVRLRTNGDIY
                     PLRATAVTEMAERRRVAQAYLNKYDVDDDEGWVDTGKIYRLDRR"
     gene            700..2004
                     /locus_tag="AC16PP_13545"
     CDS             700..2004
                     /locus_tag="AC16PP_13545"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR6482700.1"
                     /translation="MKFSARTATAVIMANMIGTGVFTSLGFQLADIRSEFVLLMLWAV
                     GGLTALCGALSYAELGAALPRSGGEYNFLGRIYHPAVGFVSGWISATIGFAAPTALAA
                     ITFGIYLASVFPALSPVWLACGLVVVLSVAHATSRRSSGGVQRAFTAVKIILILGFCL
                     LAALLVDEPQGISLLPHEGDGALLTSGAFAVALIYVNYAYTGWNAATYLTGELDRPEQ
                     TLSRVLLQGTVVVMLLYVLLNATFLYVAPAEAMEGRLEIGYIAAEHAFGSNGAAIMAV
                     ILALLLISTASAMIMAGPRVLQVIGQDYPAFRFLARVSGDGVPVTAIVVQSVLALAFI
                     LTASFEAILVFAGFTLGVSTFFTVLGLFVLRIRQPDLPRPYRTTWYPLPPLVYLAITG
                     WTLGYIIAHRPKEGLWGLAIIAGGALLYLLIRRGRPTEEVSS"
     gene            2001..3137
                     /gene="epmB"
                     /locus_tag="AC16PP_13550"
     CDS             2001..3137
                     /gene="epmB"
                     /locus_tag="AC16PP_13550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HIR6482701.1"
                     /translation="MSRRPTRDRDRDRLRYETADDCAILVPDVNPMTQSAPEQSAARL
                     RTRTVADDPATGSWQEALRNAIRTPRELIEALELDPAWAPVMDGHDDDFPMLVPRSFA
                     ARMRKGDPHDPLLRQVLPDGAERITAPGFEADPLQEQRFARAGVVRKYSGRALLIASG
                     ACPVHCRYCFRRNFPYADQLASRQRWTGAVDALSADTQVTEVILSGGDPLTLTNEAIG
                     ELIRAMENVASVQTLRFHTRFPIIIPERVDAGLVDTLAATRLRTVFVVHCNHANEIDA
                     SVRRALADLKAAGAQLLNQSVLLKGVNDNEDDLAELSQRLFDAGVLPYYLHLLDPVAG
                     TAHFDVAPARGRALVAGLRRRLPGYLVPRLAREVPGESSKTILA"
     gene            3186..3602
                     /gene="arfB"
                     /locus_tag="AC16PP_13555"
     CDS             3186..3602
                     /gene="arfB"
                     /locus_tag="AC16PP_13555"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HIR6482702.1"
                     /translation="MSVSRIRQIPPEACSERFVRSPGAGGQNVNKVATTVQLRVDLDR
                     ANLPPAVRERLEKLAGRQVNQRGELVITASRFRTQARNRADAMSRLSELLEQAQRVPR
                     RRVATRTPAGERVRRKDTKRKRGTTKRLRARPPMDD"
     gene            complement(3621..5159)
                     /locus_tag="AC16PP_13560"
     CDS             complement(3621..5159)
                     /locus_tag="AC16PP_13560"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6482703.1"
                     /translation="MEVRTLMARAAAHYADREAVIHGDRRLTFREAWQRSLRLGNGLR
                     GLGLTPGDRIGVLEDNSVEAADLFGGAAVANLVRVPLYPRNGRDAHRHMLGHTNCRAL
                     IVSENHAEEVAGLADELPDLEHVIIRDAGYDGWLAAQTDEDPRLPISSDDYFIIRHTG
                     GTTGLSKGVAYTHKAWLAAGRDWFYAFPPVEPGDKCLHLGPISHGSGYQYLPIWLAGG
                     CNVMMDHFDTGETLDLIEREGIAYAFMVPTMVNAVVHDNSARGRDFSSLKCLLIAAAP
                     IQDATALAAREVFGDVLWQGYGQTEVVPVSMMGPRQWFAEVEGSSPLRACGLPLPFAE
                     VQIWDEDNQPVAAGEVGEIVARADGQMTGFWNNPEASAERIVDGWVKTGDLGRLDANG
                     YLYVVDRADDMIISGGYNIWPAELENVLASHPAVIEVAAFGVPSQKWGESPYAVCVVG
                     NAEAVGEAELIDLVAEELGSYKKPVGVTITTDPLPKSPVGKIKRKDLREPFWKGHERR
                     VAGS"
     gene            5359..>5583
                     /locus_tag="AC16PP_13565"
     CDS             5359..>5583
                     /locus_tag="AC16PP_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6482704.1"
                     /translation="MTYPWNYGDLLDAVEHITPGSRAALIHGDRIVSWADFSRRTNNL
                     ARALLNGGAAPGDKIAFYMRNRPEYSEGIAA"
BASE COUNT          926 a         1923 c         1788 g          946 t
ORIGIN      
        1 agaacctcgc ccgccgcggg tcgaggcttc ggcaagtcca ccaggcggaa atctccaccg
       61 ggctccgtaa gctgcatcgc gcgcacgtcc acgccctccc atacgcaagc gaccggaccc
      121 tcagatcata cggtccgtgg gccgatcacg gcgagaaatc tatttacagg cggcaaagaa
      181 ttcgccctaa ttgccgtctt ctcgaaggtc aggccatggc gcgactcgca ctcacaggcg
      241 caatcatcat cctcctgagc ggctgtgcag agccgttcat cgtcttcgcc gggggggcgc
      301 tcagcggcga cgagaaggtg ccgccggccg actggtcctt tctggaagcc atcgataccg
      361 tgcagataga gacggatccg aacgacccct actccgtgaa cgtgtggtcc gtcgggatcg
      421 gcgcagacgt gtacgtggcc acgggcgagg acggcaccaa ctggacggag cgaatcgacg
      481 gtgacccgag cgtaaggctc aggaccaacg gcgacatcta cccgctgcgc gcgactgccg
      541 tcaccgagat ggccgagcgc aggcgcgtcg ctcaggccta cctgaacaag tacgacgtcg
      601 acgatgacga aggctgggtc gataccggca agatctatcg tctggaccgg cgctagtcgc
      661 gaccggctca ccgtgacggg tgcctgaggc cccgggctca tgaaattcag cgcgcgcaca
      721 gccacggccg tgatcatggc caacatgatc ggtaccggcg tgttcacgag cctgggcttt
      781 cagctggccg acatcagatc cgagttcgtc ctgctgatgc tctgggcggt gggggggttg
      841 acggcgctct gcggggcgct gtcctacgcg gagctcggcg cggctctgcc gcgctcgggt
      901 ggcgagtaca acttcctggg acgaatctat catccggccg taggcttcgt gtccggctgg
      961 atctccgcca ccatcgggtt tgccgcaccc acggcgctcg ccgccattac cttcggcatc
     1021 tatctggcct cggtctttcc cgcgctgtcg ccggtctggc tggcgtgcgg tctggtcgtg
     1081 gtgctttccg ttgctcacgc cacctcgcga cgctcgagcg gcggcgtcca gcgggcgttc
     1141 actgccgtga agatcatcct gattctcggt ttctgcctgc tcgccgccct gctggtcgac
     1201 gagcctcagg ggatatcgct gctgccccac gagggtgacg gagcgctgct caccagcggc
     1261 gcgttcgccg tggctttgat ctacgtcaac tacgcctaca ccggctggaa cgcggccacc
     1321 tacctcacgg gcgagctgga ccgccccgag cagacactct cccgggtgct gctccaggga
     1381 accgtggtcg taatgctgct gtacgtgctt ttgaatgcaa cctttctgta cgtcgcgccg
     1441 gcggaggcca tggagggcag gctggagatc ggctacatcg ccgccgagca cgccttcggc
     1501 tcgaacggcg cggccatcat ggccgtcatt ctggctctgc ttctgatatc caccgccagc
     1561 gccatgatca tggcgggtcc aagggttttg caggtgatcg gccaggacta ccccgcgttt
     1621 cgttttctgg ctcgggtgag cggcgacggg gtaccggtga cggccatcgt cgttcagtcc
     1681 gtgctggcgc tggccttcat tctcacagcg tctttcgaag ccattctcgt gtttgccggc
     1741 ttcaccctcg gggtcagcac gttcttcacg gtgctgggcc tgttcgtgct gcgcatccgc
     1801 cagcccgacc tgccccgccc ctatcgcacg acgtggtacc cgctaccgcc gctcgtctac
     1861 ctggccatca cgggatggac cctcggctac atcatcgccc accgaccgaa agaggggttg
     1921 tggggcctgg ccatcatcgc aggaggcgcg ctcctctacc tcctgatccg gcgggggcgg
     1981 ccgacggagg aggtctcttc ttgagtcgac ggccgacccg ggaccgggac cgggaccggc
     2041 tgcgctatga gaccgcagac gattgcgcga tactcgtccc ggacgtgaac cccatgactc
     2101 aaagtgcacc cgagcaatcc gcagcccgtc tgcggacacg cacggtcgcc gacgatccgg
     2161 cgaccggcag ctggcaggag gcgttgcgca acgccatcag aacgccccgg gagctgatcg
     2221 aggccctgga gctcgacccg gcctgggcgc cggtgatgga cggtcacgac gatgactttc
     2281 ccatgctggt tccgagatcc tttgcggcac gcatgcgcaa gggagatccc cacgaccccc
     2341 tgctgcgcca ggttctcccc gatggggcag agcgcatcac ggcgccgggc ttcgaagccg
     2401 acccgctgca ggagcagcgc tttgcccgag ccggcgtcgt gcgaaagtat tccggccgcg
     2461 cgctgctgat cgcctccggc gcgtgtcccg tccactgccg ctactgcttc cgcaggaatt
     2521 tcccctacgc ggaccagctt gcgtcacgcc aacgctggac gggcgccgtc gacgcgctga
     2581 gcgcggacac gcaggtgacg gaagtcatac tttcaggcgg cgatccactc acgctgacca
     2641 acgaggcgat cggcgagttg attcgcgcca tggagaacgt cgcaagcgtc caaaccctgc
     2701 ggttccacac ccgcttcccc atcatcattc ccgaacgggt cgacgcgggc ctggtcgata
     2761 cgctggccgc aacccgcttg cggacggttt tcgtcgtgca ctgcaatcat gccaacgaga
     2821 tcgacgcctc ggtgcgacgg gcgttggcgg acctcaaggc cgcgggcgcc cagcttctga
     2881 accagtccgt gctcctcaag ggagtcaacg acaacgagga tgacctggcc gagctgagtc
     2941 aacggctgtt cgatgccggc gtgctgccct actacctgca ccttctggat ccggtggccg
     3001 gcaccgccca cttcgacgtg gcgccagcgc gcgggcgtgc cctggtggcc ggtctgcgtc
     3061 gcaggctgcc cggctatctg gtgcccaggc tggcacgcga ggtgcccggc gaatcgagca
     3121 agaccatcct cgcctagcgt gccggcccgc gtctggaaag aaggcgccgc tgctatgatt
     3181 ggccgatgag cgtttcacgt atcaggcaga tcccgcccga ggcttgctcc gagcgtttcg
     3241 tccggagccc gggcgcgggt gggcagaacg tcaacaaggt cgccaccacg gtgcagctcc
     3301 gtgtcgatct cgatcgcgcc aacctcccgc cggccgtccg cgaacgactg gagaagctgg
     3361 ccgggcgcca ggtgaatcag cgcggggagc tcgtcatcac ggccagccgc ttccggaccc
     3421 aggcgcgcaa ccgggccgac gcgatgagcc ggttgagcga gctgctggaa caggcgcagc
     3481 gcgtgccccg acgccgggtt gccaccagaa cgccagccgg ggagcgggta cgacgcaagg
     3541 acaccaagcg caagcggggg accaccaagc gcctccgggc caggccgccc atggatgact
     3601 gagggccagg cctgaaccgg tcagctgccg gcgacgcgcc gctcgtgtcc cttccagaag
     3661 ggctcgcgaa gatccttgcg cttgatcttc ccgaccgggc tctttggcag gggatccgtg
     3721 gtgatggtca cgccgacggg tttcttgtag ctgcctagct cctcggcaac cagatcgatg
     3781 agctcggcct cgccgaccgc ctcggcattc ccgaccacgc aaacggcata cggcgactcg
     3841 ccccatttct ggctgggcac gccgaatgcc gccacctcga tgacggccgg atggctggcg
     3901 agaacgttct cgagctccgc cggccagatg ttgtagcccc ccgagatgat catgtcgtcc
     3961 gcccggtcca ccacgtacag atagccattg gcatccagcc tgccgagatc gcccgtcttg
     4021 acccagccat ccacgatacg ttcggcactc gcctcgggat tgttccagaa acccgtcatc
     4081 tgcccgtccg cgcgggccac gatctcgccc acctcgcccg cagccaccgg ctggttgtcc
     4141 tcgtcccaga tctgcacctc tgcaaacggc agcggcaggc cgcatgcccg cagagggctg
     4201 gagccctcca cctcggcgaa ccactggcgc gggcccatca tggacaccgg caccacctcc
     4261 gtctgaccgt agccctgcca gagcacatca ccgaagacct cacgggccgc cagtgcggtc
     4321 gcgtcctgga tcggcgcggc cgcgatgagc aggcatttca ggctgctgaa atcgcgcccc
     4381 ctggccgagt tgtcatggac gacggcgttc accatggtcg gcaccatgaa cgcgtacgcg
     4441 attccttcgc gctctattag atccagggtt tccccggtgt cgaagtggtc catcatcacg
     4501 ttgcagccgc cggcgagcca gatgggcagg tactgatagc ccgagccgtg ggagatgggg
     4561 cccagatgca ggcacttgtc gcccggctcc accgggggaa acgcgtaaaa ccagtcgcgc
     4621 cccgcggcga gccaggcctt gtgggtgtag gccactccct tggagaggcc cgtcgtgccg
     4681 ccggtatggc gaatgatgaa gtagtcgtcc gaggagatgg gcagacgcgg gtcctcgtcg
     4741 gtctgggccg cgagccagcc gtcgtagccc gcgtcgcgga tgatgacgtg ctcgaggtcg
     4801 ggcagctcgt ctgcgagccc ggccacctcc tccgcgtgat tctcgctgac gatcagcgcg
     4861 cggcagttgg tgtggccgag catgtgccga tgggcgtcgc ggccgttgcg cgggtaaagg
     4921 ggcacgcgca ctaggttcgc caccgcagcg ccgccgaaga ggtcggccgc ctccaccgag
     4981 ttgtcctcta aaacgccgat gcgatccccc ggggtgagcc ccaggccacg aaggccgttg
     5041 ccgagccgca gggaacgctg ccaggcttcg cggaaggtca gccgacggtc gccgtgaatg
     5101 accgcctcgc gatcggcgta gtgcgcggcc gcccgagcca tcaacgtacg gacctccatg
     5161 atctcccctc cttccgggcc cgatcgcctc agcagggcga ccgaccagcg cgccgctcag
     5221 accggcggca ggacaagcgc aaaagcctac cacccttgat cggcatggca atgtcgggta
     5281 tttcatcgtc cgcagggaca cttccgagca ttgggatcaa cagatgagct tcacgtcatt
     5341 gttagcgagg cacgggcgat gacctatccg tggaactacg gtgacctgct cgacgcggtc
     5401 gagcacatca cgccagggtc acgagccgcc ctgatccacg gcgatcgcat cgtctcctgg
     5461 gccgatttct cccgaagaac caacaatctg gcccgggcgc tgctgaacgg cggagcggcg
     5521 cccggcgaca agatcgcctt ctacatgcga aaccggcccg agtacagcga gggcatcgcc
     5581 gcc
//
LOCUS       DBNHKV010000417         2981 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_909611, whole genome shotgun sequence.
ACCESSION   DBNHKV010000417 DBNHKV010000000
VERSION     DBNHKV010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_909611"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /locus_tag="AC16PP_13570"
     CDS             <1..1422
                     /locus_tag="AC16PP_13570"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6482705.1"
                     /translation="ESYSFTADLELDLENVFIKTFLNYHDQTVGWVIDADNNEKTNIF
                     ASENNQFQNFSFETRAATRFDGPVNAVLGFYYQSTDRKFRQEVIFAGAEDSTADPTNR
                     FIAYDKISETDGETVSGYGELIWDVSEQWQVTAGARYIWENKDSFFTQPYVNFAFSGL
                     FVAGTLEDDSSFDDFIPEVTVRYQPNDDVTVYAAYKQGFKSGGFDNGAIYSTLNPDPL
                     ADITFEPETVEGFEAGIKAALFDGTVNVELDAYYYEYEELQLNFFNSSVFAYRTLNAG
                     AAETTGSELQVKWAPPGVDGLTLLASVAYNDASYKDFIAPCSGGQSFSGGCNIGTPPV
                     PFGPPTLMDQNLKGEPRNLAPEWAGIVGFDFTRGIGSGLQIGLTGNMQWKSDYSLSEF
                     IPSARQDGFVWIDAALRFGAESGRWQLAFIGKNLTDEYVLVSARDTAQTGGNTGTDFA
                     YPADRIGTALRPRTFEMELSVRF"
     gene            1438..2769
                     /locus_tag="AC16PP_13575"
     CDS             1438..2769
                     /locus_tag="AC16PP_13575"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6482706.1"
                     /translation="MVTNARDWRSEPAPAWAQPDVPAHHIEGSRYTDRGFAAAEWDGM
                     WSRVWLLLGRAREMPNPGDYQVEDVGPESFIMVRQDDGSIRAFYNVCQHRGSRLIFNA
                     AGSVERISCPYHSWTYGRDGALLVVQDPEDFSSGNPCERLSLVEVRCETFAGWVWVNM
                     DPDCVSLREYLGPVWEDWQTWQPEDWRRSLAISAWVPCNWKVIQDNFCESYHLLTVHP
                     QLTANIEEGVPWTRFDMANEGHNRMIMQAGMPSHRQAQGPKLVEPLLSQMAMWELDPD
                     DFAGREHDTRAAIQQQMRDLGAPRGYAHYDRLRDEQLTDPHHYNIFPNCSVTFGADSV
                     LLQRMRPHPTDPQQCRFDHWTYVAPAAIEHGVFRNSDGRVEFTGDAEIMEVEYGKESM
                     GPIADQDIGITTGQQLGLRSRGYRGANLADQESRIARFHGVIDEYIAGERP"
BASE COUNT          552 a          966 c          954 g          509 t
ORIGIN      
        1 gagtcctaca gcttcacggc tgatctggag ctcgatctgg agaatgtgtt catcaagacg
       61 ttcctcaact atcacgacca gaccgtgggc tgggtcatcg acgcggacaa caatgagaag
      121 accaacatct tcgccagcga gaacaaccag ttccagaact tctccttcga gacccgcgcg
      181 gccacccggt ttgacggccc cgtcaacgcc gtgctgggct tctactacca gagcacggac
      241 cggaagttcc gccaggaggt gatcttcgcc ggcgcggagg actccacggc ggatccgacc
      301 aatcgcttca tcgcctacga caagatcagc gagaccgacg gcgagacggt ctccggctac
      361 ggggagctga tctgggacgt ttccgagcag tggcaggtca cggcaggcgc gcgctacatc
      421 tgggagaaca aggactcgtt tttcacccag ccctacgtga acttcgcttt ctcagggctc
      481 ttcgtggcgg gcactttgga agacgacagc tcctttgacg acttcatccc tgaggtgacc
      541 gtccgttatc agcccaacga cgacgtcacc gtttacgcgg cctacaagca gggcttcaag
      601 tccggcggct tcgacaacgg cgccatctac agcactctga acccggaccc gctggccgac
      661 attaccttcg agccggagac ggtggagggc ttcgaagccg ggatcaaggc ggccctgttc
      721 gacggtactg ttaacgtgga gctcgacgcc tactactacg agtacgaaga gctgcagctg
      781 aacttcttca actccagcgt gttcgcttac cgtacgctga acgccggcgc ggcggagacc
      841 acgggcagcg agctgcaggt gaaatgggcg ccgccgggcg tcgatggcct gacgctcctg
      901 gcctcggtgg cctacaacga tgcgagctac aaggacttca tcgccccgtg cagcggcggg
      961 cagtccttca gcggcggctg caacatcggc accccgcccg tgccctttgg tcctccgacg
     1021 ctgatggatc agaaccttaa aggcgagccg cgcaacctgg cgcccgagtg ggcgggcatc
     1081 gtgggcttcg acttcacccg gggcatcggc agcgggctgc agatcggcct caccggaaac
     1141 atgcagtgga agtcggacta ctccctgagc gagttcatcc cgtcggcgcg tcaggacggc
     1201 ttcgtctgga tcgacgcggc gctgcgcttc ggcgctgaga gcggccgctg gcagctggcc
     1261 ttcatcggca agaacctgac cgacgagtac gttctggtca gcgctcgcga tacggcacag
     1321 accggtggca acacgggcac ggacttcgcc taccccgcgg accggatcgg cacggcgctg
     1381 cggcccagaa cgttcgagat ggaactgagc gtgcgcttct agcgcggagc ggagctattg
     1441 gttaccaacg cccgcgactg gcgatcggag cctgcgcccg cctgggcgca gcccgacgtg
     1501 cccgcgcatc acatcgaagg cagcaggtac acggaccgcg gctttgccgc tgcggagtgg
     1561 gacgggatgt ggagcagggt ctggctgctg cttggccggg cccgggagat gccgaacccg
     1621 ggcgactacc aggtggagga cgtcgggccc gagtccttca tcatggtgcg ccaggacgac
     1681 gggtccatcc gcgcgttcta caacgtctgc cagcaccggg gctcgcggct gatcttcaat
     1741 gccgccggtt ccgtggagcg catctcctgc ccttaccact cgtggaccta cggccgtgac
     1801 ggcgcgctgc tggtcgtgca ggaccctgag gatttcagct ccgggaatcc ctgcgagcgg
     1861 ctgagcctgg tggaggtgcg ctgcgagacc ttcgccggct gggtgtgggt caacatggat
     1921 ccggactgcg tctcgctgcg cgaatacctc ggtcccgtgt gggaagactg gcagacgtgg
     1981 caacccgagg actggcgccg gtccctggcc atctccgctt gggtaccctg caactggaag
     2041 gtcatacagg acaacttctg cgagtcctac cacctgctga ccgtgcaccc gcagctcacg
     2101 gcgaacatcg aggaaggcgt gccgtggacg cgcttcgaca tggccaacga aggccacaac
     2161 cgcatgatca tgcaggcggg catgccgtcc catcgccagg cccaggggcc aaagctcgtg
     2221 gaaccgctgc tctcccagat ggccatgtgg gagctcgacc ccgacgactt cgccggccgg
     2281 gagcatgata cccgggcggc gatccagcag cagatgcgcg atctcggcgc tccgcggggc
     2341 tatgctcact acgaccgcct gcgcgacgag cagctcaccg atccgcacca ctacaacatc
     2401 ttccccaact gctcggtgac cttcggtgcc gactctgtgc tgctgcagcg gatgcggcct
     2461 catcccaccg atccgcagca gtgccgtttc gatcactgga cctacgtggc accagccgcc
     2521 atcgagcacg gggtgttccg caactcagac ggccgggtcg aattcacggg cgacgccgag
     2581 ataatggaag ttgagtacgg gaaggaatcc atgggtccca tcgccgatca ggacatcggc
     2641 attaccaccg ggcaacagct cgggctgcgc tcgcgcggtt accggggcgc caatctggca
     2701 gaccaggagt cgcgcatcgc acggttccat ggggtgatcg acgagtacat cgccggcgag
     2761 cggccttgag cggcgatcgg gaaacggctg ccaatccggc ttcggtgagc ggaagagagc
     2821 gacatgggtg actacgttta cccgggcatt ctgagcagcc tggcgctgct cgtttactac
     2881 ttcaccctgt tcaaggccgg catggcccgg ggcaaataca acataccggc gccgtctcat
     2941 gagggccctg aggagtacgt ccgcagggtt cgcgtgcatc a
//
LOCUS       DBNHKV010000418        11289 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1122323, whole genome shotgun sequence.
ACCESSION   DBNHKV010000418 DBNHKV010000000
VERSION     DBNHKV010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11289)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11289)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11289
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1122323"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="AC16PP_13580"
     CDS             complement(<1..806)
                     /locus_tag="AC16PP_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HIR6482707.1"
                     /translation="MLDIKVLRDDPESVRDRLAVKGYLLDVSRVQALESERKRLQELT
                     ETLQNERNTRSKAIGKAKAAGEDIAPLLAEIGDLGDRLDAAKADFDALQETYQGFLRE
                     IPNLPDASVPAGNDENDNQEIGQWGERRSFEFEPADHVALTEAGGLDFDTAAKITGSR
                     FVVMHGPIARLHRALVQFMMDVHVIEHGYREVYVPYIVNAESLMGTGQLPKFAEDQFR
                     LEGSNEFYLVPTAEVPVTNIYRDTILAADQLPVRHVCHTPCFRSEAGSYG"
     gene            complement(833..1207)
                     /gene="crcB"
                     /locus_tag="AC16PP_13585"
     CDS             complement(833..1207)
                     /gene="crcB"
                     /locus_tag="AC16PP_13585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HIR6482708.1"
                     /translation="MTANALVWVALGGALGAAGRFLIGTWLFVPGQFPWATLTINIAG
                     SLGIGLLWGLGHQQPWFEQWGRYLIVVGLLGGFTTFSAFSLETVGLVEGQRWGGAVVY
                     VVASVVGCIVAAWAGQRLVGYY"
     gene            complement(1204..2493)
                     /locus_tag="AC16PP_13590"
     CDS             complement(1204..2493)
                     /locus_tag="AC16PP_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR6482709.1"
                     /translation="MSLGDENAPLAERLRPNCLEEFVGQAHLLGAGKPLRTLAESGRL
                     HSMLLWGPPGTGKTTLAKLLAARAGARWISLSAVLSGVKDVRAAVAEAEALRGLGETT
                     VLFVDEVHRFNKAQQDAFLPHVEKGTVTFIGATTENPSFEVNAALLSRARVYVLKPLG
                     EEDLNRVIDRAAEQALNLPVTPDARALLTALADGDARRMLNLLEVAAQLAEKEVDEAV
                     VTEAAGSRYRRFDKGGDAFYDQISALHKSVRGSAPDAALYWLCRMLDGGCDPLYVARR
                     VVRMATEDVGNADPRALQIALAAWDTYERLGSPEGELALAQAVLYLASVPKSNAAYVA
                     YGRAMGFVREGASEAVPMHLRNAPTRLMKDLGHGRDYRYAHDEPEHYAAGETYWPDGL
                     DPQAFYEPSDQGLEAKIRERLSRLRRLDQNTSGRAGR"
     gene            complement(2477..3106)
                     /gene="lolA"
                     /locus_tag="AC16PP_13595"
     CDS             complement(2477..3106)
                     /gene="lolA"
                     /locus_tag="AC16PP_13595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein periplasmic chaperone
                     LolA"
                     /protein_id="HIR6482710.1"
                     /translation="MKSWFRIATDPVVWLVAALSCASAAADPRQALSERLAAMDVFVA
                     EFSQEIRGSQGQVLERSTGRFFLDRPQFKWEVDHPYPQTVVTDGDRLKVYDADLEQLT
                     IRPLAEALKDTPVSLLTREGVALGNEFQVVEVTDELGASYILTPNAPDTLYAQIRLHF
                     SDNALAALAITDHLGQFTEILFSTSAEPPVIQSSEFQLVVPPGTDVIGG"
     gene            complement(3078..5330)
                     /locus_tag="AC16PP_13600"
     CDS             complement(3078..5330)
                     /locus_tag="AC16PP_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR6482711.1"
                     /translation="MTTSTIPVREIGLIGFTAAALYTMLAMVSYSPADPAFSFTGTGG
                     SVDNLVGRSGAWFADFTLYLFGRVAYILPVALVVIGVRLFRERSETFSWWLLALRCAG
                     WLAVLIFGCILARIHFAPAAALPGGAGGILGEALVALGLPVFGWIGLTLIALTAVMIG
                     AQAAAGFSWLSVAELTGRWVHAFTANAVLWLDRQLDRWRRFREARSGAKEMMRAREEA
                     LGDERAKRGSRAKPTIKPAAAQKASTVAVQEPLFESTPSGDLPGLDLFDERQPGDDRG
                     YSDESLEAISRLLELKLKDFGVEAEVVSVLPGPVVTRFEIQPAAGVKVQKISSLVKDL
                     ARSLAVISVRIVEVIPGKSVVGIEIPNEHREVVRLKEVFSSAVFQDSKSPLTLALGKD
                     IAGESVVADISRMPHLLVAGTTGSGKSVGVNAMLLSILCKATPEDVRMILVDPKMLEL
                     SVYEGIPHLLTPVVTDMKEAAHALNWCVAEMERRYKLMAALGVRNLAGYNRQIADAAK
                     KGKTISDPLWDADSEGEPAALGKLPLIVVFIDEFADMMMIVGKKVEQLIARIAQKARA
                     AGIHLVLATQRPSVDVITGLIKANIPSRISFQVSSKVDSRTILDQGGAEQLLGHGDML
                     YLPPGSGLPTRVHGAFVSDEEVHRVVSDWKQRGEPDYDNGVLSGGDDEPFLSIEPDPG
                     SAEQEDALYDEAVAFVLESRRASISSVQRKLRIGYNRAARLIEAMEAAGVVSAMNSNG
                     SREVLVPNRD"
     gene            5496..5795
                     /locus_tag="AC16PP_13605"
     CDS             5496..5795
                     /locus_tag="AC16PP_13605"
                     /inference="COORDINATES: protein motif:HMM:NF038106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4642 family protein"
                     /protein_id="HIR6482712.1"
                     /translation="MRPDKTKVVDEVWDDERIEGFLHKAPLGDERSADYSALLYAYRS
                     MRPDDFATFIRLFVDAGRDVNAKSNGGETLLATIRDHRHGAPFRAILEKAGGSAS"
     gene            5814..6518
                     /gene="aat"
                     /locus_tag="AC16PP_13610"
     CDS             5814..6518
                     /gene="aat"
                     /locus_tag="AC16PP_13610"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011533432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HIR6482713.1"
                     /translation="MSGLSVLSSHRVGFPPPESALAEPNGLLAVGGSLTPEWLLAAYA
                     RGIFPWFDDDASPLLWWSPDPRAVLLPEELRVTRSLAKRIRNAGFDVTMDQAFADVVA
                     ACAAPRGAEAGTWITADMARAYAELHEMGFAHSVETRLDGELVGGLYGVSLGRMFFGE
                     SMFARQRDASKVALYHLTLRARDWNFSLIDCQIMNEHLRSLGAREMPRAEFLRLVHEN
                     RAEPTRRGSWADETAR"
     gene            6515..7225
                     /locus_tag="AC16PP_13615"
     CDS             6515..7225
                     /locus_tag="AC16PP_13615"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003108766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HIR6482714.1"
                     /translation="MKDETADKQFFITPAHPCSYLPKRNAQTLFLDPRETITPQTYQQ
                     LTEQGFRRSGGHLYRPHCKTCQACVPTRLPVAEFVARRSQRRTLARNTDLAVRVEPAA
                     YSPAHYSLYASYIAGRHGDGDMYPPSPDQFRSFLLSQWSDTLFVNSFLDGKLVAVAVT
                     DKLDDGLSAIYTFFDPALEQRSLGVQSILQQIELARELGLSYLYLGYWIRDAEKMRYK
                     TDYRPTELFVNGRWVRIT"
     gene            7307..7522
                     /gene="infA"
                     /locus_tag="AC16PP_13620"
     CDS             7307..7522
                     /gene="infA"
                     /locus_tag="AC16PP_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR6482715.1"
                     /translation="MAKEEQIEMEGTVIDTLPNTMFRVELDNGHVVTAHISGKMRKNY
                     IRILTGDKVKVELTPYDLSKGRITFRK"
     gene            complement(7526..9817)
                     /gene="clpA"
                     /locus_tag="AC16PP_13625"
     CDS             complement(7526..9817)
                     /gene="clpA"
                     /locus_tag="AC16PP_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HIR6482716.1"
                     /translation="MLSKDLEVTLNEAFRAAKAKRHEFMTVEHLLLALLDNKVALDVL
                     EKVGVDVDRLRKDLSEYIESTTPLIPAGDSDRETQPTLGFQRVLQRAVFHVQSSGRKE
                     VTGGNVLVAIFSEQESQAVFFLKQQNVARIDVVNYIAHGISKVFGEGESGEPAAEQEE
                     EGAGEEAQQSALEAFATNLNDQARNGRIDPLVGRDAELERVIQVLCRRRKNNPLLVGE
                     SGVGKTAIAEGLAKRIVDGQVPEVVSDSTIYALDMGALLAGTKYRGDFEKRFKLVLAE
                     LKKQEGSILFIDEVHTIIGAGAASGGVMDASNLLKPLLTSGDIRCMGSTTYQEYRGIF
                     DKDRALSRRFQKIDITEPDVNDTYNILKGLKSRFEDHYHIRYTDKALRAAAELADRYI
                     TDRFQPDKAIDVIDEAGAAQLLLAPSKRKKSIGATDVEQVVAKIARIPSTQVTSSDKE
                     ALRNLDAKLKMVVFGQDEAIDSLASAIKLSRAGLKSGEKPIGSFLFAGPTGVGKTEVC
                     RQLANIMGVELLRYDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAVTKHPHSIVLL
                     DEIEKAHPEVFNLLLQVMDHGTLTDNNGRKADFRNVVLVMTTNAGAQDMSRPSIGFTQ
                     QDHTSDAMEAIKKLFTPEFRNRLDGIIQFHSLPKEVIKTVVDKFLIELQAQLDDKKVT
                     LEVDDAAREWLVVEGYDEKMGARPMQRLIQERIKRQLAEDVLFGALSKNGGTVYVTVK
                     DGDLHLRCEAPEGSADKTPAATT"
     gene            complement(9851..10189)
                     /gene="clpS"
                     /locus_tag="AC16PP_13630"
     CDS             complement(9851..10189)
                     /gene="clpS"
                     /locus_tag="AC16PP_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017641761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HIR6482717.1"
                     /translation="MQAQSGEGDADDSSDSGLATVTAKPELKRPPMYKVVLLNDDYTP
                     MEFVVHVLEIFFAMDREKATQIMLTVHTQGAAVVGIYPRDIAETKSEQVNQYAQENQH
                     PLISTIEMTD"
     gene            10480..10800
                     /gene="cspD"
                     /locus_tag="AC16PP_13635"
     CDS             10480..10800
                     /gene="cspD"
                     /locus_tag="AC16PP_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein CspD"
                     /protein_id="HIR6482718.1"
                     /translation="MAMGKVKWFNNAKGYGFILPDGGGDDLFAHYSSIQMDGYKTLKA
                     GQDVEFDILEGPKGLHAINIRAEAIESEISGEDAPVDESAAADPGPAEASPPARETSR
                     EATS"
     gene            complement(10790..>11289)
                     /locus_tag="AC16PP_13640"
     CDS             complement(10790..>11289)
                     /locus_tag="AC16PP_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR6482719.1"
                     /translation="EFGATPLDGGPWHELTNPRTGKRVIIKDVIADAMLQQILTRPDE
                     YDVIATMNLNGDYLSDALAAQVGGIGIAPGANIGDTIALFEATHGTAPKYAGQDKVNP
                     GSLVLSAEMMLRHMGWREAADLIIQGVEGAIGERTVTYDFERLMSGATLLKCSEFGQA
                     IVAKM"
BASE COUNT         2069 a         3713 c         3452 g         2055 t
ORIGIN      
        1 ttgccgtagc tgcccgcctc gctgcgaaag cagggcgtat ggcagacgtg acgaacgggc
       61 agctgatcgg ctgccaggat cgtgtcccgg tagatgttgg tgacgggcac ctccgccgtg
      121 ggcaccagat aaaactcgtt gctcccctcc aggcgaaact gatcctcggc gaacttcggc
      181 agctggcccg tgcccatcaa cgactccgcg ttgacgatgt acggcacgta gacttcacgg
      241 tagccgtgct cgatgacgtg gacgtccatc atgaactgaa ccagcgcccg atgcagacgc
      301 gcgatcggcc cgtgcatgac gacgaagcgg ctgccagtga ttttcgctgc cgtatcgaaa
      361 tcaagcccgc cggcctctgt cagggccacg tgatcggcgg gctcgaactc gaaggaccgc
      421 cgttcgcccc actgcccgat ctcctggttg tcgttctcat cattgcccgc cggcacggac
      481 gcgtccggca ggttgggaat ctcccgcaga aacccctgat aagtctcctg gagcgcatcg
      541 aagtcggctt tggccgcatc caaccgatcg ccgagatcgc cgatctcggc cagcagcggg
      601 gcgatgtcct cacccgccgc cttggccttg ccgatggcct tggatcgggt attgcgctcg
      661 ttctgcaacg tctcggtcag ctcctgcaga cgctttcgtt cgctttcgag ggcttgcacg
      721 cgagagacgt ccagaaggta acccttgacg gcaagccgat cgcgaacgct ttccggatcg
      781 tcccgcaata ccttgatgtc tagcatggcg tcttcgcctc ttgccgatat gctcagtaat
      841 agccgaccag gcgctggccg gcccaggccg cgacgatgca gccgacaacg ctcgcgacaa
      901 cgtaaacaac ggcgccgccc cagcgctggc cctccacgag gcccaccgtt tccagcgaga
      961 acgccgagaa cgtggtaaac ccaccgagca ggccgacgac gatgaggtag cgaccccact
     1021 gctcgaacca gggctgctga tgacctaaac cccagagcaa cccgatgccg agcgagcccg
     1081 cgatgttaat ggttagggtg gcccatggga attgaccggg cacgaacagc caggtgccga
     1141 tcaggaaccg ccccgcggcg cccagcgcgc cgccgagagc aacccagacc agagcgttcg
     1201 cggtcacctt cccgcccggc cggacgtgtt ttgatcgagc cggcgcaggc ggctcagccg
     1261 ctcgcggatc ttcgcttcga ggccctgatc ggaaggctcg tagaatgcct ggggatccaa
     1321 cccgtcgggc cagtaggtct ctccggctgc gtagtgctcg ggctcgtcgt gggcataacg
     1381 gtagtcacgg ccgtgaccca gatctttcat cagtcgggtg ggggcattgc gaaggtgcat
     1441 gggcaccgct tcagacgccc cctcgcgcac gaagcccata gcccgaccgt aagcgacgta
     1501 agcggcgttg ctcttgggca cgctggcgag atacagcacc gcctgggcga gcgccagctc
     1561 cccctcaggc gagcccaggc gttcataggt atcccacgct gcaagggcga tctgcagggc
     1621 ccgagggtcc gcattgccga cgtcctccgt cgccatgcgc accaccctgc gggcgacgta
     1681 gagcggatcg caaccaccgt ccagcatgcg gcacagccag tagagcgcgg cgtccggagc
     1741 actgccccgc acggatttgt gcagcgcgga gatctggtcg tagaaggcat cgcccccctt
     1801 gtcgaaccgc cggtagcggc tgccggcggc ctccgtcacc acagcctcgt ccacctcctt
     1861 ctcggccagc tgagcggcca cctcgagcag gttcaacatc cgccgcgcgt cgccgtcggc
     1921 cagcgcggtc agaagcgcgc gagcatcagg ggtgaccggc aggttcagcg cctgctccgc
     1981 cgcccgatcg atgacccggt tgagatcttc ctcacccaaa ggcttgagca cgtaaaccct
     2041 tgcccgggac aagagcgcag cattcacctc gaaagatggg ttctccgtgg tcgccccgat
     2101 aaaggtcacg gtgccctttt ccacatgggg cagaaaggca tcctgctggg ccttgttgaa
     2161 ccggtgcacc tcgtcgacga acagcaccgt ggtctcgccc aatccccgca aggcttccgc
     2221 ctcagccacg gcggcccgaa cgtctttcac cccgctcagc acggcagaga ggctgatcca
     2281 tcgagcaccg gcccgggcgg ccagcagctt cgccagggtg gtcttgccgg tacccggcgg
     2341 cccccagagg agcatggaat gaaggcggcc ggactctgcc agggtccgca ggggctttcc
     2401 ggcgcccaga agatgcgcct gacccacgaa ctcctccagg cagttcggcc tgaggcgctc
     2461 ggctagcggt gcgttctcat cccccaatga catcggttcc cggcggcacg acgagctgaa
     2521 actcgctcga ttgtatcacc gggggttcgg ccgacgtgct gaagaggatc tccgtgaact
     2581 gaccgagatg atccgtgatg gccagcgcgg ccaaagcgtt gtcggagaaa tgcaggcgaa
     2641 tctgcgcata cagggtgtcg ggcgcgttgg gcgtgaggat gtaggaagca ccaagctcgt
     2701 cggtgacctc caccacctga aactcgttgc ctagggctac gccctcgcgg gtaagcagcg
     2761 acaccggcgt gtccttcagc gcctccgcca gaggacggat cgtcagctgc tcgagatccg
     2821 catcgtaaac cttcagccga tctccgtcgg tcaccaccgt ctgcgggtaa gggtggtcga
     2881 cttcccactt gaactgcggc cggtcgagaa agaaccgtcc ggtggagcgc tccagcacct
     2941 ggccctgaga gccgcgaatc tcctggctga actccgccac gaacacgtcc attgcggcca
     3001 gccgttcgga tagcgcctgc cgggggtcgg ccgccgccga ggcgcaggat agcgcggcga
     3061 cgagccagac gacggggtca gtcgcgattc ggaaccagga cttcacgact gccgttgctg
     3121 ttcatggcgc ttaccacgcc ggctgcttcc atggcttcta tgagccgcgc ggcacggttg
     3181 tagccgatcc ggagcttgcg ctgcacggat gagatggacg cgcgccgcga ctcgaggacg
     3241 aaggccaccg cctcgtcgta cagggcgtcc tcctgctccg ctgagccggg atccggctcg
     3301 atggagagaa aaggctcgtc gtcgccgccg gacagcacgc cgttgtcgta atcgggttcg
     3361 ccccgctgtt tccagtcgga gactacccgg tgcacctcct cgtcggagac gaacgcaccg
     3421 tgcacacgag tcggcagtcc agagcccggt ggcaggtaga gcatgtcccc atgacccaga
     3481 agctgctcgg ccccgccctg atcgaggatc gtgcgcgagt ccaccttgga cgacacctga
     3541 aagctgatgc gcgaaggaat gttggccttg atgagacccg tgatcacgtc caccgacgga
     3601 cgctgcgtcg ccaggaccag atggatcccg gccgctcgcg ccttctgggc gatgcgggcg
     3661 atcagctgct ccaccttctt gccgacgatc atcatcatgt cggcgaactc gtcgatgaac
     3721 acgacgatga gcggcagctt gccaagagcc gccggctcgc cttccgaatc ggcgtcccac
     3781 agcggatccg agatcgtctt gcccttcttg gcggcgtctg caatctgacg gttgtagccg
     3841 gccaggttgc gcacgccgag cgccgccatg agcttgtagc gtcgttccat ctcggccacg
     3901 caccagttga gcgcgtgcgc cgcctccttc atgtccgtca ccaccggcgt gaggaggtga
     3961 ggaatcccct cgtacaccga cagctccagc atcttgggat ccaccaggat catccggacg
     4021 tcttccgggg tggccttgca gaggatgctc agcagcatgg cgttgacccc gacggactta
     4081 cccgaacccg tggtgccggc caccagcagg tgaggcatgc gggagatgtc ggccaccacg
     4141 gactcgccgg cgatgtcctt gcccagggcc agggtgagcg gcgatttcga gtcctgaaag
     4201 acggccgagc tgaaaacctc cttcaaccgc accacctcgc ggtgctcgtt ggggatctcg
     4261 atgcccacca cggatttgcc gggaatcacc tccacgatcc gcacgctgat caccgccagg
     4321 gaacgggcca ggtccttcac cagggagctg attttctgga ccttgacccc cgcagccggc
     4381 tgtatctcga accgtgtcac cacggggccg ggcagcacgg agaccacctc ggcctctacg
     4441 ccgaagtcct tgagcttgag ctcgagcagt cgcgagatcg cctcgaggga ttcatccgag
     4501 tacccgcgat cgtccccggg ctgacgctcg tcgaaaaggt ccaatcccgg cagatcgccg
     4561 ctgggcgtgg attcgaaaag cggttcctgc acggcaaccg tcgacgcttt ctgcgcggcg
     4621 gcgggtttga tggtcggctt cgcgcggctc ccgcgcttcg cgcgctcgtc gcccagggct
     4681 tcctcccggg cacgcatcat ctccttagcg cccgagcggg cttcgcgaaa acgacgccag
     4741 cggtccagct gtcgatcgag ccagagcacg gcgtttgcgg taaaggcgtg cacccacctt
     4801 cccgtcagct ccgcgacgct cagccaggag aaaccggcgg ccgcctgggc gccgatcatc
     4861 accgcggtca gcgcgatgag cgtaagcccg atccagccga acacgggcag cccgagggcg
     4921 accagggctt cccccaatat gccacccgca ccgccaggca aggccgccgc cggagcaaag
     4981 tgaatgcgcg cgaggatgca gccgaagatc agaaccgcca gccagccggc gcagcgcagg
     5041 gcgagcagcc accacgaaaa cgtttcgctg cgttccctga agagccgaac gccaatgacc
     5101 accagcgcaa cgggcaggat gtaggcaact cttccgaaca gatacaaggt gaagtcggcg
     5161 aaccaggcgc cactgcgacc caccagattg tcgacgctcc cgccggtgcc cgtgaacgag
     5221 aacgcgggat cagcgggtga gtaggaaacc atggcgagca tggtgtacag ggcggcggcc
     5281 gtgaatccaa tcaggccgat ttcccggaca ggaatcgtcg atgtggtcac gaatttctcc
     5341 ttaaattcgg cgcgaagtgt agcggcaaaa ccatctgcga acaaatctct atttaattca
     5401 attcgttacc cgaactacct caatcagaac ttgccgactt accggcagtc caaccaatcg
     5461 ttatcatagc caccgaacct gaacacgggc caaacatgcg accagacaaa acgaaagtcg
     5521 tcgacgaggt gtgggatgac gagcgcatcg aagggttcct gcacaaagcg ccgctgggcg
     5581 atgagcgcag cgccgactac tcagcgctgc tgtacgccta ccgcagcatg cggcccgacg
     5641 atttcgccac gttcattcgc ttgttcgttg acgccggacg ggacgtcaac gcgaaaagca
     5701 acggcggaga aaccctgctt gccaccatcc gcgaccaccg ccatggcgcg cctttccgcg
     5761 ccattctgga aaaggccggc ggtagcgcga gctgaagcga agcgcctaga acgatgtccg
     5821 ggctaagcgt gctatcaagc caccgtgtgg ggtttccccc accagagtcc gccctcgccg
     5881 aacccaacgg cctgctggcc gtgggcggca gcctgacgcc ggaatggctg ctggcagctt
     5941 acgcgcgcgg catctttccc tggttcgacg acgatgcatc accccttctc tggtggagcc
     6001 ccgacccccg ggccgtgctc ttgccggagg aactgcgggt aacgcgcagc cttgccaaac
     6061 gaattcgcaa tgcaggtttc gatgtgacca tggaccaggc gtttgcggat gtggtcgcag
     6121 catgcgccgc gccccgcggc gcggaggccg gcacctggat caccgcggac atggcacgcg
     6181 cctacgccga acttcacgag atgggctttg cccactcggt ggagacccgg ctggacggcg
     6241 agctggtggg ggggctctac ggcgtttctc tggggcgcat gtttttcgga gagtccatgt
     6301 ttgccaggca gcgggacgct tcgaaggtcg ccctgtacca tctgaccctg cgcgcccggg
     6361 actggaattt ctctctcatc gactgtcaga tcatgaacga gcacctgcgc tccctaggcg
     6421 cccgggaaat gccccgagcc gagttcctcc ggctagtcca tgaaaaccgg gccgagccta
     6481 cgcgccgcgg atcgtgggcg gacgagacgg cccgatgaaa gacgagaccg ccgacaagca
     6541 gttcttcatc acgcccgccc acccctgcag ctacctgcct aagcgcaacg cccagacgct
     6601 ctttctcgat ccccgggaga cgatcacgcc gcaaacctac cagcagctca ccgagcaggg
     6661 ttttcggcgc agtggcggcc atctttaccg gcctcactgc aagacctgcc aggcctgcgt
     6721 gcccacgcgc ctccccgtcg ccgagttcgt tgcgcgccgg agccagaggc gaaccctggc
     6781 acgcaatacg gacctggccg ttcgggtgga acccgccgcg tacagccccg cccattactc
     6841 gctttacgcc agctacatcg caggccgcca tggggatggc gacatgtacc caccctcccc
     6901 cgaccagttc cgttcttttc tcttgagtca gtggtcggac accctgttcg tcaacagctt
     6961 cttggatggc aagctcgttg ccgtggcggt cacggacaag ctggatgatg ggctttctgc
     7021 catctacact ttcttcgatc cggcgctaga gcagcgcagc ctgggtgtgc agagcatcct
     7081 ccagcagatc gagctcgccc gggaactcgg gctgtcctat ctgtatctgg gctactggat
     7141 ccgcgacgcg gaaaaaatgc gctacaagac cgactaccgc cccaccgagc tgttcgtgaa
     7201 cggacggtgg gtgcgtatca cctgagcttt gcatgtgccc ggaccatgag gcagaatgcc
     7261 gcacgcaaaa ttcgggccgc ccgtaaacgg acggcgtcag gagtatatgg ctaaagaaga
     7321 acagatcgag atggagggaa cggtgatcga caccctcccc aacaccatgt tccgagttga
     7381 gctggacaac gggcacgtgg tcaccgcgca catctccggc aagatgcgca aaaattacat
     7441 tcgcatcctc accggcgaca aggtcaaggt agagcttacc ccctacgacc tcagcaaagg
     7501 ccgcatcacc ttccgcaaat aaacttcaag tggtcgccgc cggcgtcttg tcggcggacc
     7561 cttcgggcgc ttcgcagcgc aggtgcaggt cgccgtcttt gaccgtgacg taaacggtcc
     7621 cgccattctt cgacagcgca ccgaacagca cgtcttcggc caactggcgc ttgatgcgct
     7681 cctgaatcaa ccgctgcatg ggccgggcgc ccatcttctc gtcgtagccc tcgacgacga
     7741 gccactccct cgcagcatcg tccacctcca gggtgacttt cttgtcgtcc agctgggcct
     7801 ggagctcgat gaggaacttg tccaccaccg tcttgatgac ctctttgggc agggagtgga
     7861 actgaatgat gccgtcgagc cgattgcgga attccggcgt gaacagcttc ttgatggcct
     7921 ccatcgcgtc cgaggtgtgg tcctgctgag tgaagccgat ggagggccgg ctcatgtcct
     7981 gggcccccgc gttggtggtc atgaccagca ccacgttgcg gaaatcggcc ttccggccgt
     8041 tgttgtcggt gagcgttccg tggtccatga cctgcagcag caggttgaag acttccggat
     8101 gggccttctc gatctcgtcc agcagcacga tgctgtgggg atgcttggta accgcctcgg
     8161 taagcaggcc gccctggtcg aagcccacgt atcccggcgg cgccccgatg agtctggaga
     8221 ccgtgtgccg ctccatgtac tccgacatgt cgtagcgcag cagctccact cccatgatgt
     8281 ttgccagctg gcggcagacc tccgtcttgc ccacgcccgt agggccggca aacaggaacg
     8341 agccgatggg tttctcaccg gacttcaggc ccgcgcggga gagcttgatg gccgaagcga
     8401 gcgaatcgat ggcctcgtcc tgaccgaaca ccaccatctt cagcttggca tccaggttgc
     8461 gcagcgcctc cttgtccgag ctggtcacct gggtcgacgg gatccgcgcg atcttcgcca
     8521 cgacctgctc cacgtcggtc gctccgatgc tcttcttgcg cttgctggga gcgagcagca
     8581 gctgggctgc gccggcctcg tcgatgacgt cgatggcttt gtccggctgg aagcggtcgg
     8641 tgatgtaccg gtctgcaagc tcggcagccg cacgcagcgc cttgtccgta tagcggatgt
     8701 ggtagtggtc ctcgaaccgt gatttcagcc ccttgagaat gttgtaggtg tcgttgacgt
     8761 ccggctcggt gatgtcgatt ttctggaaac gccgggacag cgcccgatcc ttgtcgaaaa
     8821 tcccgcgata ctcctgatag gtagtggacc ccatgcagcg gatgtcgccg gaagtcagaa
     8881 gcggtttgag caggttcgac gcgtccatca cgccgccgct ggcagcaccg gcgccgatga
     8941 tcgtgtgcac ctcgtcgatg aagaggatgc tgccttcctg ctttttcagt tcggccagaa
     9001 cgagcttgaa acgcttttcg aaatcgccgc gatacttggt gcctgccagc agcgcgccca
     9061 tgtcgagcgc gtagatggtg ctgtcggaga ccacctcggg cacctggccg tccacgatgc
     9121 gcttggccaa accctcggcg atggccgtct tccccacgcc cgactcgccg acgagcagcg
     9181 ggttgttctt gcgccgccgg caaagcacct gaatgacccg ctccagctca gcatcccggc
     9241 ccacgagcgg atcgatgcgc ccgttgcgtg cctgatcgtt gaggttcgtt gcgaaagctt
     9301 ccagggcgct ctgctgggcc tcttcgccgg cgccttcctc ttcctgctcg gccgcgggct
     9361 ccccggattc tccttcgccg aagaccttgg agatgccatg ggcgatgtag ttaacgacgt
     9421 cgatgcgcgc gacgttctgc tgcttcaaga agaacaccgc ctggctttcc tgctcgctga
     9481 atatcgcaac gagcacgttc ccgccggtga cctccttgcg ccccgacgac tggacgtgga
     9541 acacggcgcg ctgcagaacc cgctggaagc cgagcgtcgg ctgggtctcc cggtcggagt
     9601 ctccggccgg aatgagcggc gtggtgctct cgatgtactc cgaaaggtcc ttgcgcaacc
     9661 gatcgacgtc cacgcccacc ttctcgagca cgtccaaggc gaccttgttg tccagcagcg
     9721 ccagcagcag gtgctcgacg gtcatgaact cgtgacgctt ggctttggca gcgcggaacg
     9781 cttcgttcag ggtaacttct aaatctttac tcagcatgag gtgtgtctgg cttcgtcctg
     9841 tgttcggtga tcaatcggtc atctctatcg tcgagatcag cggatgctga ttctcctgag
     9901 catattgatt cacttgctcg gatttagttt cagcaatgtc gcggggatag atccccacca
     9961 ccgccgcgcc ctgggtgtgc accgtgagca tgatctgcgt cgctttctcg cgatccatgg
    10021 caaaaaagat ctccagcacg tgcacgacga actccatggg cgtgtaatcg tcgttcagca
    10081 gcaccacctt gtacatcggc gggcgcttga gttccggctt ggcggtgacc gtcgcgagcc
    10141 ccgaatcgga gctgtcgtcc gcgtcaccct cgccggattg cgcttgcaac gggtcgctct
    10201 tcagaaccct gggcggtatc tgggaacgat ttgttggctc cattgctcct cgttcatgtg
    10261 ggcggcagca cgatgataca agcatccccg ggccttgact aagcccgaaa atccatggaa
    10321 agtcgggctc ataggggagc tgcaacttgt gaccgttcga ttatacgcgg cgggacgggt
    10381 acaaagatgc gcaacactgc gcgcttcgcg cagtttgggc ggcagggccg aggtacgcac
    10441 aaggatgggc gttgtgttcg tggctgctag ggggtctcca tggcgatggg aaaagtaaag
    10501 tggtttaaca atgccaaggg gtacggattt atcctgcccg acggcggtgg cgacgatctg
    10561 tttgcgcact actcttccat tcagatggac ggctacaaga ctctcaaggc cggccaggac
    10621 gtggagttcg acattctgga gggccctaaa ggcctgcacg ccatcaacat acgcgcggag
    10681 gcgattgaat ccgagatttc aggcgaggac gcccccgtcg acgagagcgc ggcagctgat
    10741 cccggcccgg cagaggcctc tccaccggcc cgagaaacct ctcgcgaggc tacatcttag
    10801 caacgatggc ctgaccgaat tccgagcact tcaacaacgt cgcgccgctc atcaggcgct
    10861 cgaaatcgta ggtcaccgtc cgctcgccaa tggcgccttc cacaccctga atgatcaggt
    10921 cggcggcctc gcgccacccc atgtggcgga gcatcatctc tgccgagagc accagcgagc
    10981 cgggattcac cttgtcctgc cccgcgtact tcggcgcggt gccgtgggtc gcttcgaaca
    11041 gcgcgatggt gtcgccgatg ttggcaccag gcgcgatacc gataccgccg acctgagcgg
    11101 ccagggcatc ggacaggtag tcgccgttca gattcatggt ggcgatcacg tcgtactcgt
    11161 ctggccgggt gaggatctgc tgcagcatgg catcggcgat cacgtctttg atgatgaccc
    11221 gcttgcccgt gcgggggttg gtcagctcat gccaggggcc gccgtccagc ggggtcgcgc
    11281 cgaactcct
//
LOCUS       DBNHKV010000419         3712 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1829947, whole genome shotgun sequence.
ACCESSION   DBNHKV010000419 DBNHKV010000000
VERSION     DBNHKV010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1829947"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1516)
                     /gene="oadA"
                     /locus_tag="AC16PP_13645"
     CDS             complement(<1..1516)
                     /gene="oadA"
                     /locus_tag="AC16PP_13645"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HIR6482720.1"
                     /translation="MSTKRPLGITDVVLRDAHQSLLATRMRVEDMLPIAGKLDQVGFW
                     SIESWGGATFDACIRYLGEDPWERMRKLKAAMPNTPQQMLFRGQNILGYRHYADDVVG
                     KFVERAAANGVDVFRIFDALNDIRNLRTAIRATLDVGRHAQGTISYTVSPVHTMEVWV
                     DLGKQIEDTGAHSIAIKDMAGLLKPYVAFELVSRLKESVSVPIHMQCHATTGLSTATY
                     LKAIEAGIDNVDTAISSMSMTYGHSPTETLVSILEGGDRDTGLDISLLEEIAAYFRQV
                     RKKYAKFEGTLKGVDSRILVAQVPGGMLTNLESQLREQNASDRLDEVLDEIPRVREDL
                     GFIPLVTPTSQIVGTQAVINVLMNERYKNVTKETAGVLKGEYGATPAPVNADLQARVL
                     NGESPITGRPADHLQPEVEALTGELQELAAERGFALAGDVVDDVLTYALFPQVGLKFL
                     ENRGNPDAFEPAPWVVEVPALEAGRVVPAPPAPPRASTYNVRVNGRQFTVEVSEA"
     gene            complement(1527..1721)
                     /locus_tag="AC16PP_13650"
     CDS             complement(1527..1721)
                     /locus_tag="AC16PP_13650"
                     /inference="COORDINATES: protein motif:HMM:NF016187.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HIR6482721.1"
                     /translation="MVQGLHLMLAGMATVFVFLSVLVGATVVMSRVLMRLQGEPGPTA
                     DEVAAIVAAIERHRASGPDR"
     gene            1924..2000
                     /locus_tag="AC16PP_13655"
     tRNA            1924..2000
                     /locus_tag="AC16PP_13655"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1958..1960,aa:Arg,seq:acg)
     gene            2051..2185
                     /locus_tag="AC16PP_13660"
     CDS             2051..2185
                     /locus_tag="AC16PP_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482722.1"
                     /translation="MNKDNRRFHGWRLIGVIMIVIIALAAVSFVVDWMVIGPLEGRVF
                     "
     gene            complement(2196..2762)
                     /locus_tag="AC16PP_13665"
     CDS             complement(2196..2762)
                     /locus_tag="AC16PP_13665"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6482723.1"
                     /translation="MRLSKLNRDDLTADQRAVLDAIQSGPRKSKHGAIGLVGPFGVWV
                     RAPRVGLATQALGAAARFDSRLPENVKEVAICTVGAFYRAKFEFAAHRELAVAAGVDP
                     EEIEKLRVGELPSFNGAEDLSYRVATSLLNLHRLDSNTYTGALGQFGEEGLIELVTII
                     GYYCMVALTLNAFEVPLTSGMDDPFPDA"
     gene            3273..>3712
                     /locus_tag="AC16PP_13670"
     CDS             3273..>3712
                     /locus_tag="AC16PP_13670"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6482724.1"
                     /translation="MIDAQGMTVMPGFIDTHRHLLLYSNAGSARQLRRYINRTLSREL
                     SQTLAQGITTALSPGDHVPEIIEIRERIASGEMPGPRLLVAGPVIQARGDHVATTVCR
                     GRSFCRKRATAEARTPEEAREHVRRAASLGVDFIKTVHDRQVAPR"
BASE COUNT          677 a         1230 c         1106 g          699 t
ORIGIN      
        1 ccgcttcgga aacctccacg gtgaattgcc tgccgttcac cctcacgttg taggtgcttg
       61 cccgaggggg tgcgggcggc gccggcacca ccctgccggc ttccaatgca ggcacttcca
      121 ccacccaggg cgcaggctcg aacgcatcgg ggttgcctcg attctcgaga aacttgagcc
      181 ccacctgggg aaacaaagcg taagtcagga catcgtccac gacgtcgcct gccagcgcga
      241 aaccccgttc cgccgcaagt tcctgcagtt cgcccgtcaa cgcttccacc tctggctgca
      301 gatgatctgc cggacgacca gtgatcggcg actccccgtt gagcacacgg gcctgaagat
      361 ccgcgttgac cggggccggc gttgcgccgt attccccctt gagcacgccg gccgtctcct
      421 tggtgacgtt cttgtagcgc tcgttcatca ggacgttgat gaccgcctgg gtgccaacga
      481 tctgggaggt gggcgtaacc agagggatga aaccgagatc ctcacggaca cgggggatct
      541 catcgagcac ctcatcgagc cggtccgagg cgttctgctc gcgcagctgg ctctccaggt
      601 tcgtcagcat cccgccaggc acctgggcta ccagaatgcg cgagtccacg cccttgagcg
      661 tgccctcgaa ttttgcgtac ttctttcgga cttggcggaa gtaggcggca atctcctcca
      721 gcagcgagat gtcgagcccg gtgtcgcgat cgccgccctc gagtatcgac accagcgtct
      781 ccgtaggcga gtgaccatag gtcatgctca tggacgagat ggccgtgtcc acgttgtcga
      841 tgccggcctc gatggccttg aggtacgtgg ccgtcgacag ccccgtcgtg gcatggcact
      901 gcatgtggat gggcacgctg accgactcct tcaggcggct caccagctcg aaggccacat
      961 agggcttgag aagcccggcc atgtccttga tggcgatgga gtgcgcgccc gtatcttcga
     1021 tctgcttgcc caggtcgacc cagacttcca tggtgtgcac ggggctgacg gtgtacgaga
     1081 tcgtcccctg ggcgtgcctg cccacatcga gcgtcgcgcg gatggcggtt cgcagattcc
     1141 tgatgtcgtt gagcgcatcg aatatccgaa aaacgtcgac cccgttggcc gccgcgcgct
     1201 cgacgaattt gcccacgacg tcgtccgcat aatgccggta acccagaatg ttctggccgc
     1261 ggaagagcat ctgctgcggt gtgttcggca tggccgcttt aagcttgcgc atccgctccc
     1321 acggatcttc acccagatac cggatgcagg catcgaaggt cgcgccgccc caggattcga
     1381 tggaccagaa cccgacctga tcgagcttgc cggcaatcgg cagcatgtcc tcgacgcgca
     1441 tgcgggtagc cagcaggctc tgatgggcgt cacgcaggac cacgtcggtg atccccaacg
     1501 gtctcttggt gctcatgggt acctcatcat cggtccgggc cggaggcgcg atgcctttca
     1561 atggccgcaa cgatggctgc cacttcgtcc gcggtggggc cgggctcgcc ctgcagccgc
     1621 atgagcaccc tggacatgac gaccgtcgcg ccgacgagaa ccgagagaaa gacaaacacc
     1681 gtcgccatgc cagccagcat caggtgcaaa ccctgaacca tcagatcaga catctccgga
     1741 ccgattgcgc cactaaaact cgggttgcgc attctgcatc aggtgcccgg gcaaaggtag
     1801 ccgcagcgcc gcgctcctat gaactgtcgg ccctttcttg acggggtttt tcgccttcca
     1861 gataatagcg cgccctcgcg gactggaacg gcaagcgata cgccatttcc ccgcctggac
     1921 cgcgcgcccg tagctcagct ggatagagcg catggctacg aaccatgagg ccgggagttc
     1981 gaatctctcc gggcgcgcca tttccatcac cgaaaccggt tccgaccgga agacgatgag
     2041 ctctgccccg atgaacaagg acaacaggcg atttcacggc tggcggctca tcggcgtcat
     2101 catgatcgtc ataattgccc tcgcggcggt cagcttcgtc gtggactgga tggtcatcgg
     2161 tccgctggaa ggccgcgtct tctgagcttg cgcggctaag cgtccggaaa cgggtcgtcc
     2221 attccggagg tcaggggtac ttcgaaagcg ttcagggtca gggcaaccat gcagtagtag
     2281 ccgatgatgg tgacgagctc gatgagcccc tcctcaccaa attgtccgag cgccccggtg
     2341 taggtgttcg agtccagccg atgcagattc agcaggctcg tcgccacccg gtaggacaga
     2401 tcctcggccc cgttgaacga gggcagctcc cccacccgaa gcttctcgat ctcctcgggg
     2461 tcgacacctg ctgcgacggc gagctcccgg tgcgcagcaa actcgaactt tgctcgatag
     2521 aaagcgccga cggtgcagat ggccacttcc ttcacgttct ccggaagacg gctgtcgaat
     2581 cgtgccgctg cacccagggc ctgagttgcg agccccacgc gcggcgcccg gacccagacg
     2641 ccaaacggac caacgagccc gatggctccg tgtttgctct tgcgcggccc gctctgaatg
     2701 gcatcgagca cggcccgttg atccgcggtc aggtcgtccc gattcagttt cgacaatctc
     2761 atgaggcacc tcccctttct ccgggatcat cttcgttcaa acgccgtgaa atatcgaatg
     2821 gccccaggcg cgggagccgc gactttcaac acgtaacaat tggttacctt atctccgttg
     2881 actgccatgg tgggggtgcc tatggtcctg cccgcagcgt cgcttcacgg cttcgcacca
     2941 agcccgaaac cacagctaac ggaccgccaa gtgatgatgc acagcaacga gactgcccgc
     3001 gacgactccg tctttgagaa tgtcgttgga gtcatggcgg ccgtgtctgt gctgtgcttc
     3061 atggggttgt tgctgttcat ctgacggccg cactactcct catatcactc ctaccgctgg
     3121 ccggatgggc cagtcccgat tccgccctga tcatcaccaa tgcccgcatc ctcgacggtg
     3181 cgggcagcgt catagacaac ggcaccgtgc acgttctggg tgagcgaatt gcggccgttg
     3241 gggccgagcc atcaagcagc gacgcatcga tcatgatcga cgcgcaaggc atgaccgtca
     3301 tgcccggctt catcgacacc catcggcatc tgctgcttta cagcaacgca ggcagtgcgc
     3361 gacagctgcg tcgctacatc aaccgaaccc tttcccgcga actctcgcag acgctggctc
     3421 agggaataac caccgccctc tcgcccggcg accacgttcc cgagatcatt gagatcaggg
     3481 agcgcatcgc gtccggtgag atgcccgggc cccgcctgct cgtggctggc cccgtgattc
     3541 aggctcgcgg cgaccacgtg gccaccacgg tatgccgagg ccgctcgttc tgccgcaagc
     3601 gcgccaccgc cgaggcgaga acgcccgaag aggcccgcga gcacgttcgc cgcgctgcct
     3661 ctctgggcgt agacttcatc aagacggtgc acgaccgtca ggtggcgccc cg
//
LOCUS       DBNHKV010000420         6948 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1477345, whole genome shotgun sequence.
ACCESSION   DBNHKV010000420 DBNHKV010000000
VERSION     DBNHKV010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6948)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1477345"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="AC16PP_13675"
     CDS             complement(<1..787)
                     /locus_tag="AC16PP_13675"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR6482725.1"
                     /translation="MTGKVGFISLGCPKALVDSEQLLTALTAEGYTTADDYSEADVVV
                     VNTCGFIDAAIEESFDAIEEALDACGKVVVTGCLGARKDSLQERYPELLSVSGPQDPV
                     PVVDAVRAHVPLSAAESTHYHLGPAGVKLTPAHYAYLKVAEGCNHTCSFCIIPSMRGK
                     LRSRALPDLLEEADRLAEVGVEELLVISQDTSAWGTDLRRSELRGLKPGEALELLCRE
                     LAARFPWVRLHYLYPYPHVDQLLDLMAEGCLLPYLDVPLQHADP"
     gene            1157..2239
                     /locus_tag="AC16PP_13680"
     CDS             1157..2239
                     /locus_tag="AC16PP_13680"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003382491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR6482726.1"
                     /translation="MMSAELENLNVAETEVLVTPAALKALLPVPDAVGESVRASRRAV
                     QNILDHSDHRLLVVVGPCSIHDTDAAREYAQRLKQLADEVSSELFVVMRTYFEKPRTT
                     VGWKGLINDPYLDDSFRVGEGLEIARRLLLDITSLGLPVSTEALDPITPQYLQDLITW
                     SAIGARTTESQTHREMASGLSCPIGFKNGTDGSLDVAINALESAVSPHRFLGISSSGQ
                     VSVIRTTGNRHAHIVLRGGSRGPNYDAESVAACEAALEKIGQVPNIMVDCSHANSNKD
                     HRRQPDVARSVAEQIENGSRSLMGLMIESNLHEGNQPVNGGSNLRYGVSVTDACIDWP
                     TTESLLRELAERLGPALRARAKVAQR"
     gene            complement(2230..2811)
                     /gene="nfuA"
                     /locus_tag="AC16PP_13685"
     CDS             complement(2230..2811)
                     /gene="nfuA"
                     /locus_tag="AC16PP_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HIR6482727.1"
                     /translation="MIEISESAQGYLRDLLANQEEEGVGIRVFVAQPGTPQAETCIAY
                     CRPGEQQEGDVAVAYDGFTAWFEERSAPYLVEAMVDYAEDRMGGQLTIKAPNARLPRV
                     GPDAPLEDRINYVLYNEINPGLAAHGGVVTLVEVLEDMTAILQFGGGCQGCAAVDITL
                     KHGVEKSLLEQIPELTGIKDATDHTMTEHAYYR"
     gene            3001..6663
                     /gene="metH"
                     /locus_tag="AC16PP_13690"
     CDS             3001..6663
                     /gene="metH"
                     /locus_tag="AC16PP_13690"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIR6482728.1"
                     /translation="MPAEPSLIQQLKERILLLDGAYGTMLQALNLDEPDYRGSIFEGH
                     PAPLKGNYDALNLARPELVADVHRAYMGAGADIIKTNSFNSTRIAQGDYALADRAEEL
                     NAAAARIARRCADEFASPEWPRFVAGVLGPTNRTASLSPDVNDPGFRNVTFEELEENY
                     YEAAQALLDNGADLLLLETVFDTLNAKAAIFAIRQLGEKRGATVPLIVSGTITDASGR
                     TLSGQTCEAFWASVNHAEPLAVGLNCALGADQLRAYVARLGRVADTYLSVHPNAGLPN
                     AFGEYDESPESMAAKLGEFARSGLVNLVGGCCGTTPAHIAAIRESVRECQPRAIPERR
                     NDLMLSGLELLTIDDASLFVNVGERTNVTGSARFARLIREEAYDEALVVARQQVENGA
                     QVIDINMDEGMLDSQAAMVRFLQLLAAEPDIARVPIMIDSSKWEIIEAGLRCVQGKAI
                     VNSISLKEGEDAFEAVARRCRDYGAAVVVMAFDEDGQADTLERKREICKRSYRILTER
                     IGFPPQDVIFDPNVFAIGTGIDEHRGYAVSFIEACRWITDNLPHARVSGGISNVSFSF
                     RGNNPIREAIHTVFLYHAIRAGLTMGIVNAGQLGIYQEIPDELREKVEDLVLNRRSDA
                     TERLLAVADRYGGRASESEGPDLSWREGSVAQRLGHALVKGIGEFIVEDTEEARLAAD
                     HPIEVIEGPLMDGMNVVGDLFGQGKMFLPQVVKSARVMKQAVAHLVPYIEAAQGPGRK
                     AKGRIVMATVKGDVHDIGKNIVGVVLQCNNYEVIDLGVMVPMEKILDTAQEVGADVIG
                     LSGLITPSLDEMVQVAGEMQRRGLNLPLMIGGATTSKAHTAVRIEPAYQDGPTVYVSD
                     ASRSVSVAARLVSPQQRIGFSEETRKEYEEIRRRTSARSANRKYLSLEAARRNAFVCN
                     WEAYEPPRPASIGVQTIENLPLETLVPYIDWSPFFLTWELAGKYPAILDDPVVGESAR
                     DLFRDASQMLEHLCREKRLKANAAFGFWPCNRTGPEELTLFEDEERKTELARLQHLRQ
                     QTIRGAGRPNYSLADFVGPPPVADYIGAFAVTAGIGIEDVLADYGDDDYGAIMIKALA
                     DRLAEAFAEYLHEQVRQVHWGYAPGESLAAEELIGEAYRGIRPAPGYPACPEHSEKST
                     LFRLLDAQANAGMTLTESFAMLPAASVAGWYFSHPDARYFGIGKIDDEQVADYASRKG
                     ITALEAEKLLRPNLDR"
     gene            complement(6660..6794)
                     /locus_tag="AC16PP_13695"
     CDS             complement(6660..6794)
                     /locus_tag="AC16PP_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482729.1"
                     /translation="MDDTPTDDARSEADRKADAWAVMIIFWALVAGAVHYVSGWTFDF
                     "
BASE COUNT         1316 a         2259 c         2214 g         1159 t
ORIGIN      
        1 ccggatccgc gtgctgcagc ggcacgtcca gataaggaag cagacacccc tccgccatga
       61 ggtcgaggag ctgatccacg tgggggtaag ggtagagata gtgcagacga acccagggga
      121 aacgggctgc gagctcgcga cacagcagct ccagggcctc gccgggcttg agcccacgca
      181 gctcgctccg gcgcaggtcg gttccccacg cgctcgtgtc ttgagagatg accagcagtt
      241 cctcgacacc aacctcagcg agacgatccg cctcctccag caggtccggc agcgctcgag
      301 agcgcagctt gccccgcatg ctcggaatga tgcagaagct gcaggtgtgg ttgcagccct
      361 cggcgacctt gagataggcg tagtgcgcgg gagtcagctt cacgccggcg gggccaaggt
      421 ggtagtgcgt ggactcagca gcgctcagcg ggacatgggc ccgcacggcg tccacgacgg
      481 gaaccgggtc ctgaggaccg ctgaccgaca ggagctccgg gtaacgttcc tgcaggctgt
      541 ctttccgcgc gccgaggcag ccggtcacca ccaccttgcc gcaggcgtcc agggcctctt
      601 cgatggcgtc aaacgactct tcgatggccg cgtcgatgaa accgcaggta ttgacgacca
      661 ccacgtcggc ttcggagtaa tcatcggccg tggtataccc ctccgccgtc aacgccgtga
      721 gcagctgctc cgagtcgacg agggcctttg gacatccgag gctgatgaac cccacctttc
      781 ctgtcatctt ctagttgccc ggcaccggtt gtgaagcgtc gcgcattgta gccgtggccg
      841 gtcgtcgggt cactgaacca ctcgagatgg acgatgcagg cgcatgcaga cgcaccgagc
      901 gcgagttgat tttcccggtc gagtgtgtaa ccgtccgcgc gccatggaag acaacgcacg
      961 acaattctgg ctccaggtct ggtcccaagg ctccaacgcc gttggcggca cgggcagcca
     1021 gggttggtat tggtttttta acggatagca ccgccttcta gaaaagggag gcagtgcagg
     1081 ctgccttcgc agggaacccc gcaaggcagc gccagcgggg ttttttttgc cctgatgaag
     1141 gcgtttcgag gagacgatga tgtcggcaga actagagaac ctgaacgtcg cggagacgga
     1201 ggtgctggtc acccccgccg cgctcaaggc gctgttgccg gtgcccgacg cggtcggcga
     1261 gagcgtgcgg gcatcccgtc gggcggtgca gaacattctc gaccactcgg atcaccggct
     1321 gctggtcgtg gtcggcccct gctccatcca cgacaccgac gccgcccggg agtacgccca
     1381 gcgtctgaag cagctggccg acgaagtatc ctccgaactc ttcgtcgtga tgcgcaccta
     1441 cttcgagaag ccgcgcacca ccgtcggctg gaagggattg atcaacgacc cgtacctgga
     1501 cgactccttc agagtcggcg agggcctgga gattgcgcgc cggctgctgc tggacatcac
     1561 cagcctgggc ctgccggtga gcaccgaagc gctcgacccc atcaccccgc agtatctcca
     1621 ggacctgatc acctggtcgg ccatcggtgc ccgcaccacc gagtcgcaga ctcaccggga
     1681 gatggcgtcg gggttgagct gcccgatcgg tttcaagaac ggcacagacg gctccctgga
     1741 cgtcgcgatc aacgctctgg aatccgccgt gagcccgcac cgttttctgg ggatcagctc
     1801 atcgggacag gtctcggtga tccgcactac cggcaaccgt catgcccaca tcgttctgcg
     1861 cggcgggagc cgggggccca actacgacgc cgagagcgtc gcggcctgtg aggcggccct
     1921 ggagaagatc ggccaggttc ccaacatcat ggtcgactgc agccatgcca actccaacaa
     1981 ggaccaccgc cgccagccgg acgtggcgcg aagcgtagcc gagcagatcg agaacgggag
     2041 ccgctccctg atggggctga tgatcgagag caaccttcac gagggcaacc agcccgtcaa
     2101 cggcgggagc aacctccgct atggcgtatc ggtgaccgat gcctgcatcg attggccgac
     2161 aacggagtcg ttgctgcggg agcttgccga gcggctgggc cccgcattgc gggcgagagc
     2221 caaagtggct caacggtagt aggcgtgctc cgtcatcgtg tggtcggtgg cgtccttgat
     2281 gcccgtcagc tcgggaatct gctcgaggag agatttctcg acgccgtgct tgagcgtgat
     2341 gtccaccgcc gcgcagccct gacagcctcc gccgaactgc aggatcgcgg tcatgtcctc
     2401 gagaacctcg acgagggtca ccacgccgcc gtgggccgcc agaccgggat tgatctcgtt
     2461 gtacaggaca tagttgatgc gatcctcgag cggcgcgtcg ggaccgaccc ggggaagccg
     2521 ggcattgggc gccttgatgg tcagctgccc acccatccgg tcctcggcgt agtcgaccat
     2581 ggcttcgacc aggtacggcg cgctgcgttc ctcgaaccag gcggtaaatc cgtcgtaggc
     2641 gaccgccacg tccccttcct gctgctctcc gggccggcaa taggcaatgc aggtctccgc
     2701 ctgaggggtg ccgggctggg ccacgaacac gcggatgccc acgccctcct cttcctggtt
     2761 ggccagcaga tcgcgcaggt acccttgcgc cgactctgaa atctcgatca tggctaggtt
     2821 ccggtgggtg cttgagctat acctgagtaa aacactcaga atagaagcgg catggtagct
     2881 gaaccccggg ggccgggcaa tcgcgcatga gcttgactca tgatcatctc cgataacttc
     2941 gattgcgctc aggttggcgg cggctagact gatacccatc aacccatgcc cgaagactcg
     3001 atgcctgccg agccctcact gattcagcaa ctcaaggagc gcatcctgct gctcgatggt
     3061 gcctacggga ccatgctcca ggccttgaat ctcgatgagc cagactatcg gggctcgatc
     3121 ttcgagggcc atccggctcc gctgaagggc aactacgatg ccctcaatct ggcccgtccc
     3181 gagctggtcg cagacgtcca tcgcgcctat atgggcgcgg gggcggacat catcaaaacc
     3241 aacagcttca actccacgag aatcgctcag ggcgactacg cgctggccga tcgcgcggag
     3301 gagcttaacg ccgccgccgc acggatcgcc cgacgctgtg ccgatgagtt tgcctcgccc
     3361 gaatggccgc gcttcgtcgc gggcgtgctc gggcccacca accgcacggc gagcctctct
     3421 ccggacgtga acgatcccgg gttccgcaac gtcaccttcg aggagctaga ggagaactat
     3481 tacgaagccg cgcaagcgct tctcgacaac ggtgcggatc tgctactgct cgaaaccgtg
     3541 ttcgacacct tgaacgcgaa agcggcgatt ttcgcgatcc gccagctggg cgagaaacgg
     3601 ggcgcgacgg tgccgctcat cgtctccgga acgattaccg acgccagcgg gcgaacgctt
     3661 tccggccaga cgtgcgaagc gttctgggcc tccgtcaatc acgccgagcc cctggccgtg
     3721 gggctgaact gcgcgctggg agccgatcag ctgcgcgcct acgtggcgcg cctggggcgc
     3781 gttgccgata cctacctcag cgtccacccc aacgcgggcc tgcccaacgc gttcggcgag
     3841 tacgatgagt cgcccgagtc catggccgcc aagctcggcg agtttgcccg cagcgggctg
     3901 gtaaatctgg tgggcgggtg ctgcggcacg acgccggcgc acattgccgc cattcgagag
     3961 tcggtgcggg aatgccagcc cagagcgata cccgagcgcc gcaacgatct gatgctcagc
     4021 ggcctggagc tgctcaccat cgacgacgcc tcactcttcg tcaacgtggg cgagcggacc
     4081 aacgtgacgg gctccgcgcg ttttgcccgg ctgatacgcg aagaggccta cgacgaagcg
     4141 ctcgtcgtag cccgccagca ggtggagaac ggggcccagg tcatcgatat caacatggac
     4201 gaggggatgc tggacagcca ggccgccatg gtgcgctttc tccagctcct cgccgccgag
     4261 cccgacatcg ctcgcgtgcc gatcatgatc gactccagca aatgggaaat catcgaggcc
     4321 ggtcttcgct gcgtgcaggg caaggcaatc gtcaactcga tcagcctcaa agagggagaa
     4381 gacgccttcg aggccgtggc tcgacgatgt cgcgactacg gcgcggccgt ggtggtgatg
     4441 gccttcgacg aggacggcca ggccgacacc ctggagcgca agcgcgagat ctgcaagcgt
     4501 tcctaccgca tcctgacgga gcggatcggg ttccctcccc aggacgtgat cttcgatccc
     4561 aacgtctttg ccatcggaac cggcatcgac gagcaccgcg gctacgcggt cagcttcatc
     4621 gaggcgtgcc gctggatcac cgacaacctg ccccacgcga gagtctcggg cggcatcagc
     4681 aacgtctcct tctccttccg cggcaacaac cccattcgcg aggcgatcca cacggtcttc
     4741 ctgtatcacg ccattcgcgc cgggctgacc atgggcatcg tcaatgccgg tcagctggga
     4801 atttaccagg agattcccga tgagctgcgc gagaaggtgg aagacctggt gctcaatcgc
     4861 cgatccgatg ccaccgagcg tctgctggcg gttgccgacc ggtacggcgg cagggcctcg
     4921 gagagcgaag ggcccgacct ttcctggcgg gaaggcagcg tggcccagcg actcggccat
     4981 gccctggtga aaggcatcgg cgagttcatc gtcgaggaca ccgaggaagc ccggctcgcc
     5041 gccgatcatc ccatcgaggt gatcgagggc cccctgatgg acggcatgaa cgttgtgggc
     5101 gacctgttcg gccaggggaa gatgtttcta ccccaggtgg tcaagagcgc gcgcgtgatg
     5161 aaacaggcgg tggcccacct ggtgccctac atcgaagccg cacagggccc gggtcggaaa
     5221 gccaaaggcc gcatcgtcat ggcgaccgtg aaaggagacg tccacgacat cggcaagaac
     5281 atcgtcggcg tcgtgcttca gtgcaacaac tacgaggtga tcgacctggg cgtcatggtg
     5341 cccatggaga aaattctcga cactgcccag gaggtgggcg cagacgtcat cgggctttcc
     5401 gggctgatca ccccctcctt agacgagatg gtgcaggtgg ccggcgagat gcaaagacgc
     5461 ggcctgaacc tgccgctgat gatcggtggc gcaacgacct ccaaagccca cacggcggtc
     5521 aggatcgagc cggcttacca ggatggcccc accgtctacg tttccgacgc ctcgcgcagc
     5581 gtgagcgttg ccgcacgcct ggtctctcca cagcagcgca tcggcttcag cgaggagacc
     5641 agaaaggaat acgaggagat ccgccggcgc acgtctgccc gctcggccaa ccggaagtat
     5701 ctcagcctgg aggccgcccg acgcaacgct ttcgtctgca actgggaggc ctacgaaccg
     5761 cccagaccgg cctcgatcgg cgtccagacc atcgagaacc tgcccctgga gaccctggtt
     5821 ccctacatcg actggtcgcc gttcttccta acctgggaac tggccggcaa ataccccgcg
     5881 atcctggacg atcccgtggt gggcgagagc gcccgggatc tgtttcgcga cgcctcgcag
     5941 atgctcgaac acctctgccg cgagaagcgc ctgaaggcga acgccgcgtt tggattctgg
     6001 ccatgcaacc gcacggggcc cgaggagctg accttgttcg aggatgagga gcgcaaaacg
     6061 gagctcgcca ggctgcagca cctccgacag cagacgatca ggggcgcggg gcgcccgaac
     6121 tacagcctgg ccgacttcgt gggaccgccc ccggtggccg actacatcgg cgctttcgcc
     6181 gtgaccgccg ggatcggcat cgaggacgtt cttgccgact acggcgacga cgactacggc
     6241 gccatcatga tcaaggccct tgccgacagg ttggcagagg cgtttgccga gtacctgcac
     6301 gagcaggtcc gtcaagtgca ctggggttac gcgcccggtg aatccctggc agccgaggag
     6361 ttgatcggtg aagcctaccg gggcatccgg cccgcgcccg ggtatcccgc gtgccctgag
     6421 cacagcgaaa aaagtacgct gttccgcctg ctcgacgccc aggctaacgc cggcatgacg
     6481 ctgaccgaaa gcttcgccat gctgccggcg gcctcagtag cgggctggta tttctcccac
     6541 cccgacgcac gatatttcgg catcggcaag atcgacgacg agcaggttgc cgactacgcc
     6601 agcaggaaag gaattaccgc cctggaagcg gagaagttgc tcaggcccaa cctcgatcgc
     6661 tagaaatcga aggtccagcc ggagacgtag tgcacggccc cggcgacaag ggcccagaaa
     6721 atgatcatca cggcccaggc gtcagccttg cgatccgctt cagatcttgc atcgtctgtg
     6781 ggggtgtcgt ccatcgtgct cctcaccagc gagttgatcg tgcccagcgc acggcgcgcg
     6841 agtctaccga aaaagacggt tctaagcata gtccgaacgg ttacttcgag cggcaagggc
     6901 cgaatgttag attcgcctgc ctcaaagatg acgagacgcg gcctgcgc
//
LOCUS       DBNHKV010000421         1728 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_344380, whole genome shotgun sequence.
ACCESSION   DBNHKV010000421 DBNHKV010000000
VERSION     DBNHKV010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_344380"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="AC16PP_13700"
     CDS             complement(<1..630)
                     /locus_tag="AC16PP_13700"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6482730.1"
                     /translation="MRHALGAPRQGQEQTNMSYADGRLINDADSHLMELPDFLTAHAD
                     PGMRGRLPELNPGGQFDMSERTHQRGHPPEVVAELTALGNDLTRGPKWHDALGASSGH
                     ERRTALDLLGFQRQVVFSSFCARLIFEAEPLDIRFAAARAHNRAMAEFCSADERLIGV
                     AMVPLDDPNAAVREIAHAHELGLGAVWIAADAPGGRSPGHPEHDPIWATL"
     gene            688..1437
                     /locus_tag="AC16PP_13705"
     CDS             688..1437
                     /locus_tag="AC16PP_13705"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6482731.1"
                     /translation="MSDAFRLDGQVAVVTGAGKGIGRGIALCLAQAGADVVVSARTLS
                     DVQGVAAEIEKLGRRALAVSADVTSAEQLETLVVAAQELGGLDIWVSNAGGLPDATPR
                     YLTRTPEDRWDAQIDLNLKAVWQGAVVAAQNMDRGVIINISSRSAQGPSFKNGPYGAS
                     KAAVNSLTQTLSLELAPNIRVNAVAPGPIPTENFNESTNFPDDKPIEQIIGVPLGRLG
                     TPEDIGNAVVFMASPAAAWITGQCLYVTGGL"
     gene            complement(1484..>1728)
                     /locus_tag="AC16PP_13710"
     CDS             complement(1484..>1728)
                     /locus_tag="AC16PP_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482732.1"
                     /translation="GSVATEFIQDHAEAQGMDLSESQTPLYVGRCAVAMLAADDLMSR
                     SGSIQWVEDLGEEFDILDEHGKRPPGYAQRPGTQAS"
BASE COUNT          296 a          537 c          610 g          285 t
ORIGIN      
        1 gagggtggcc cagatgggat cgtgctcggg gtgcccggga gaacgtcctc cgggcgcatc
       61 ggcggcgatc cacacggcgc ccaggccgag ctcgtgagcg tgggcaatct cgcgcacggc
      121 ggcgttgggg tcgtcaagcg gcaccatggc cacgccgatc agccgctcgt ctgcactgca
      181 gaattcagcc atcgctcggt tgtgcgcccg ggccgcggcg aagcgtatgt ccagaggttc
      241 cgcctcgaag atcagccgcg cgcagaacga agagaacacg acctgacgct gaaaaccgag
      301 caggtccagg gcggtacgac gttcgtgccc gctggaagcg ccaagcgcgt catgccattt
      361 cggcccgcgc gtcaggtcgt tgccgagcgc cgtaagctcg gcgacgacct cgggtgggtg
      421 accgcgctgg tgggttcgct cgctcatgtc gaactggcct cccggattga gctcgggaag
      481 acggccacgc atgcccgggt ccgcatgggc agtgaggaag tccggcagct ccatcaggtg
      541 gctgtcggca tcgttgatca gacgcccatc ggcgtagctc atgttggtct gctcctgacc
      601 ctggcgcggc gcgcctagtg cgtgccgcat gaatcatgcc gggaagggtt tagtatagta
      661 cgaagctttc aaagagaggg tgtgacgatg tccgacgcgt ttcgactgga cggccaggtg
      721 gcggtggtta cgggggctgg taagggaatc ggccggggaa tagcgctttg ccttgcacaa
      781 gcaggggcag acgtggtggt ttcggcccgc acgctgagcg acgttcaggg ggtggctgca
      841 gagatcgaaa agctcgggcg tcgggcgctg gccgtatcgg cggacgtcac cagcgcggag
      901 cagctcgaga cgctggtggt ggcggcacag gagctgggcg ggctcgacat ctgggtcagc
      961 aatgcgggcg gtctgccaga cgcgactccg cgatacctga ctcggacgcc ggaggaccgc
     1021 tgggatgcgc agatcgacct caatctcaaa gccgtgtggc agggtgccgt cgtcgcggcg
     1081 cagaacatgg atcgcggtgt catcatcaac atatcctcac gatccgctca gggcccttct
     1141 ttcaagaacg ggccttacgg tgcctccaag gcggccgtga acagccttac ccagacgctt
     1201 tcgctggagc tggcgccgaa catccgcgtg aacgccgtag cgcccggacc gatcccgacg
     1261 gagaacttca acgaatccac gaacttcccg gacgacaaac ccatcgagca gatcatcggg
     1321 gtgcccctgg gacggctcgg cacgcccgag gacatcggca atgccgtcgt gttcatggcc
     1381 tcgccggcgg cggcctggat aacggggcag tgcctctacg tgaccggcgg gctttaggcg
     1441 atcgcccgtc ttccccggag caaggagagg accgggcccc gccttacgag gcctgggtgc
     1501 ccggccgctg agcgtagccg ggcgggcgct tgccgtgctc gtcgaggatg tcgaactcct
     1561 cgccgaggtc ttccacccac tggatcgacc cgctgcgtga catgaggtcg tcggcggcca
     1621 gcatcgcaac ggcgcaccgc ccgacgtaga ggggcgtctg ggattcggaa agatccatcc
     1681 cctgggcttc cgcgtggtcc tggatgaact ccgtggcgac ggagcccg
//
LOCUS       DBNHKV010000422        13411 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1548175, whole genome shotgun sequence.
ACCESSION   DBNHKV010000422 DBNHKV010000000
VERSION     DBNHKV010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13411)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13411)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13411
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1548175"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="AC16PP_13715"
     CDS             <1..912
                     /locus_tag="AC16PP_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011266417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR6482733.1"
                     /translation="GVSEETTTGVHRLYQMMEEGQLLFPAINVNDSVTKSKNDNRYGC
                     RHSLNDAIKRGTDMLMAGRRALVIGYGDVGKGSAQSLRQEGMIVRVVEIDPICAMQAC
                     MDGFEVVSPYLGGENTGRIENIDTRLLHDTDLIVTCTGNADVCDASMLSTVKAGAVVC
                     NIGHFDNEIDTAFMREHWRWEMVKDQVHQVFRSDDRHDYLILLSEGRLVNLGNAMGHP
                     SRVMDGSFANQVLAQMHLYEQRWADQPENQRAPITVEVLPKKLDEEVAELMVAGFGGV
                     VTRLTAEQAKYIGVDESGPFKPESYKY"
     gene            922..1308
                     /locus_tag="AC16PP_13720"
     CDS             922..1308
                     /locus_tag="AC16PP_13720"
                     /inference="COORDINATES: protein motif:HMM:NF013304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HIR6482734.1"
                     /translation="MTTPLWCLLIVMLMPIVLAGVGGYFRNKQLGTLDNDDPREQIKS
                     LTGAGSRAYAAQQNAWEALALFTAAALTAHVLGADPERSAQLAVAFVVFRVLHGVCYI
                     GGWATLRSLMFMGSLVSLIWLFVLGA"
     gene            1336..2040
                     /locus_tag="AC16PP_13725"
     CDS             1336..2040
                     /locus_tag="AC16PP_13725"
                     /inference="COORDINATES: protein motif:HMM:NF015605.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6482735.1"
                     /translation="MQGLPEVSIGALLVAFLPSLALLAVMRAWSLQAGQALIANIRML
                     VQLLAIGYVLVFVFETENPWIVAAVLVMMMGVAAWIALRPLQSRSARKYLQSLTAISI
                     CGLAMLALVSQLVIDLPRWFEPRFVVPLAGMIFANAMNTVSLAAERFESEQAREVEYA
                     EARTTALDAALIPQVNALLAVGLVSLPGMMTGQILSGVDPLIAVRYQIVVMCMIFGSS
                     GLAAVAYLFAQRPRGA"
     gene            complement(2051..2527)
                     /locus_tag="AC16PP_13730"
     CDS             complement(2051..2527)
                     /locus_tag="AC16PP_13730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6482736.1"
                     /translation="MPSRRELIALTEGEIRDYLAEQKTLIIVSNGKDGYAHPVPMWFW
                     TDPDGCLYCTTFSKAQKVLNFRRDPKASLLVESGDEYTELKSVLIYAKAEVIEEFDAV
                     REALVNISTKGRGDVSEAERAQIRDAVGKTAEKRVLLKFTPERYVSWDHAKLGGRY"
     gene            2631..3503
                     /gene="tesB"
                     /locus_tag="AC16PP_13735"
     CDS             2631..3503
                     /gene="tesB"
                     /locus_tag="AC16PP_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HIR6482737.1"
                     /translation="MSQDQTVTDLIDLLDLEEIEKNHYRATSPSEGWQRVYGGQVLGQ
                     ALVAASRTVPEDRHAHSLHGYFLRGGDTTVPILYTVDRIRDGRSFTTRRVVAIQHGKA
                     IFNMAISFQIDEPGLSHQMPMPEVPDPEGLPDESELRKAWADKLPEPLEAAFDRERPI
                     EVRPIDPVDVFAPEKRPPQQMCWMKCRDRLPDDPGLHQCVLAYLSDWSLLDTATLPHG
                     VSFMQDNMQMASLDHAMWFHRPFRADEWLLYVQDSPSASGARGLNRGLIYNRAGELVA
                     SAAQEGLIRLHETD"
     gene            3628..4578
                     /locus_tag="AC16PP_13740"
     CDS             3628..4578
                     /locus_tag="AC16PP_13740"
                     /inference="COORDINATES: protein motif:HMM:NF012234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6482738.1"
                     /translation="MAEAAVNIEELTESVGSLRDGLESHRYICSDQIATAVYLAYHLH
                     KPILIEGPPGVGKTELAKTTAEMFALPLIRLQCYEGLDEAKAIYEWKYGKQLLYTQVL
                     KETLDEVLQGAKGFAESVTRLHEFGDIFFSEEFLEPRPLLKALKEEAGCILLIDEVDK
                     SDHEFESLLLEMLSDFQVSIPEIGTVKAVRQPPMVFLTSNNTREISDALKRRCLHLYI
                     PFPDVDLEQRIIHARVPEIPPELRRQLVQFIHELRDLDLKKVPAISETIDWARTLLLL
                     HTESLDPKLVRDTLNVILKFQEDIDNVDAEVTTLTSKALK"
     gene            4585..6084
                     /locus_tag="AC16PP_13745"
     CDS             4585..6084
                     /locus_tag="AC16PP_13745"
                     /inference="COORDINATES: protein motif:HMM:NF017569.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR6482739.1"
                     /translation="MDRTLTNFIRALRNSEVRISTAETLDAFNTVELVGYRDRAALKR
                     SLALVLPKTADEKETFEQCFDAYFSFTDIQGSQGAAGEAERASDAETTAGGEEGGEGG
                     GAQPGEARPGDAAGSAKKKQKPGASADAEADEDEDLGAGAMSKPQSTLGQLLMANSKV
                     ELSVATAAAGEAVDVRDIEVFTQKGLYTRRIMEEMGLADLNREISELRQRPAVPDRRL
                     GQELTRRRDWLREQVRDYVEKQFLLHADASGKRLREELLRKVKLSNVEQRSFRLIQEI
                     VFRMAKKLAALHSRRKKVFKRGQINIPRTLRHNMAYDGAIFDLRWKSVKIDRPKVFAI
                     CDVSGSVANYARFMLMFLYSLEEVMPKVRSFAFSSDLAEVTELFERNAIEDAIAKTIR
                     DYSGGSTDYGQSLMDFKKLCLDEVDNRTTIIILGDARNNYGDPHTELLKELYDRCKRL
                     IWLNPEPRNAWGVGDSEMRRYAAYCHQVEECNSLMHLERIVGNLLRVVQ"
     gene            6098..7792
                     /locus_tag="AC16PP_13750"
     CDS             6098..7792
                     /locus_tag="AC16PP_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6482740.1"
                     /translation="MTTVAGSEDTFNRSAGRQLLLFAAISIGSIGLLMYALAWASSLT
                     GSGAGSEVAVDPETRTVTLFLREEPPQLNSSLATDMVSGMVLGHVMEGLLRYDEHNRL
                     VPGMAERWEIDANGAIFHLRSDAVWSDGAPVTAHDFVFAWQLALDPANASQYAFILYP
                     VKNGRAIAEAKLPVSQLGARAVDDRTLEVTFERPVAYFDKLVAFPTYYPIRRDFYEAT
                     KGRYGADAHTLLYNGPFRMTRWVHGAHVRLEKNPTYWDEERIRIEVLDFPYITSDANA
                     TLNLFKDNKVAYAGLLAENLNDAMEQRWHIKRFMDGSVFFIEFNFRDGRVTANTNLRK
                     AMQHAVDPAELVYKVTKLPGYIPGESLFPVWLRGVNGYFRQEYPAPKTIKDVVKAREY
                     LQAAKRELGMEQIPPLVLLSGDNPVSNIQSEYFQQVFKQTLGLDIRIDKQIFKQRLAK
                     MTSGDFDMVLAGWGPDYDDPLTFGDLFASWNLNNRGRYSNPELDRQVAIAEESLDPKT
                     RMDAFGEIQRILHDDVVILINYEPGTTYVVNPDLGGLVRRSVGPDPDFTNAYIVEG"
     gene            7803..8735
                     /locus_tag="AC16PP_13755"
     CDS             7803..8735
                     /locus_tag="AC16PP_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6482741.1"
                     /translation="MLNYTLKRLLQGIVTVWFIATATFIAMHSVPGDPLADEKAVTAE
                     IRANLEAKYGLDKPVFDQYLIYMGNIVKGDFGISFTQENREVNDIIREHFPVSATLGI
                     LAVVFAGLGGVLWGALTALYRNRLPDVIIMFLVILGISVPSFVIAAISQLSLVNLNDL
                     LGVSILPVAGWGSVWHMLVPSMVLGLGTMAYLTRLMRSSMLEIVNADYVRTAKSKGLP
                     PTRIFTRHQLRNAILPVITVLGPSIAAITTGGFVVELVFAIPGLGRYFVQAVQQLDYT
                     VIMGTTVFYGSFLVFMVIVVDLLYGLIDPRVRLE"
     gene            8739..10106
                     /locus_tag="AC16PP_13760"
     CDS             8739..10106
                     /locus_tag="AC16PP_13760"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6482742.1"
                     /translation="MAETALTARDFEPVHAERRIQTISRPSLSYWQDAWIRLKANRRA
                     LFSLYIVLGLLAFTIAGPFVWTVDPAAQDVDQISQPPGTDRRAAVVEPYQPWDGSSVL
                     PLTEGLRLAEPATNQAVRLMWDATPGAAGYVIYRNLFPVGPENALGLPLGENFSAEDT
                     KFEDLLDLRPRRYYYSVVSLGAAGNELAQETLMVNVDRVITVAEAEARGMGEGRQLAV
                     GDIVRLPLHPLGTDYLGRDLLARLMEGARVSLFIGICAPFLYVLFGVAYGSAAGFLGG
                     HFDQALMRFADFVIALPFLLFMILFKIAFGIGPGESGIMPMLAAMVLLSWPGTSRLVR
                     GQILQIREEGYISASRLLGAKTGYLVLRHMIPNTMGVILVTLTFAVPSAIFTEAFLSF
                     IGMGVAPPTPSWGSMCNEGIRTMLTTPHELIFPALFISVTVLAFNLLGDGLRDALDAR
                     MRSTE"
     gene            10103..11128
                     /locus_tag="AC16PP_13765"
     CDS             10103..11128
                     /locus_tag="AC16PP_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020289227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6482743.1"
                     /translation="MSTPRLLEVEDLKVEFDTYGGVVQAVRGVSFGVDEGETLAIVGE
                     SGCGKSVTVQSIMGLTPMPPGRITSGSARLRGTDIIGRKEIGGEDIRGGQIGMIFQDP
                     MTSLNPTMNIGSQIAETLEVHRGYSKKQAFQRAVELLDMSRIPEAASRARQYPFEFSG
                     GMLQRAMIAMAIACEPAILIADEPTTALDVTIQAQILDLMLDLQRETGMAIILITHDL
                     GVVARMADEVAVMYAGQIAEQGTVDDVFYRSSHPYTLGLRAAMPNNDPARQHELKPID
                     GSPPDLFHPPKGCAYAARCPYAMRICESEDPHAYHMTERHFARCWQHHEGAAPRVEGL
                     YYREARA"
     gene            11125..12096
                     /locus_tag="AC16PP_13770"
     CDS             11125..12096
                     /locus_tag="AC16PP_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010428636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6482744.1"
                     /translation="MSERPVLLETDHLTKHFLIGRGQEVHAVDGVSLAIHEQEIVGLV
                     GESGSGKSTYGKTVVGLHDKTSGTVTYRGERLPQRYGAADFQKHARHMQMIFQDPYSS
                     LNPRMTVGEIIGEGLRLHANLDVREVRERVAEWLKRVGLEPDHMSRYPHEFSGGQRQR
                     IGVARALILEPEFVVCDEPISALDVSVQAQVINLLDDLKRSMGLTMLFIAHDLSMVRY
                     VSDRMAVMYLGSVVEIGPANDVFFNPKHPYTEILVGSNPEPDPEAERHRASTAIVGEI
                     PSPVNVPEGCRFANRCPKVMDVCRSVTPELIPLKNAEAVREVACHLY"
     gene            complement(12321..>13411)
                     /locus_tag="AC16PP_13775"
     CDS             complement(12321..>13411)
                     /locus_tag="AC16PP_13775"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR6482745.1"
                     /translation="LESWAIFGELYWDITDSLSSTIGLRYSDEKKSSKQRTCYVTFLQ
                     CGPFDPEGENGYFFPKYDDNEVTWKVNLTYDVNDDLMVYGTVSSSYKSGGFNPISDSD
                     QLVIDDPSNATFEPETIDAFEVGFKSTLLDRTMQLNGSFFYYDYKDLQVSKIVDVTSL
                     NKNTDAKILGAEVEVLWAPIENLQFDLTGSWLDSEIKDFEDYDTADPKGDGSTDGLIV
                     CLASVFLDTSPNGCGPGRIPGVLQSQDGNQLAQSPEFSFNIGARYRVPMGGLGLTVAT
                     NFYWQDSFYVRNNNALHDKVESWDVWNARARFAPNDERWYAEAWIKNILDNDYVVQQY
                     LSTQVSGLFTNQFLLDPQTFGLTVGFNW"
BASE COUNT         2406 a         4478 c         4217 g         2310 t
ORIGIN      
        1 ggagtgtcgg aggagacgac cacgggggtc caccggctct accagatgat ggaggagggc
       61 cagctgctct tcccggccat caacgtcaac gattcggtca ccaagtccaa gaacgacaac
      121 cgttacggct gccgccacag cctgaacgac gccatcaagc gcggcaccga catgctgatg
      181 gccggcaggc gcgcgctggt catcggctac ggtgacgtcg gcaagggctc ggcccagagc
      241 ctccgtcagg agggaatgat cgtgcgcgtc gtggagatcg atcccatctg cgccatgcag
      301 gcttgcatgg acgggttcga ggtggtttct ccctacctgg gcggcgagaa caccggccgc
      361 atcgagaaca tcgatacccg cctgctgcac gacaccgatc tcatcgtcac ctgcaccggc
      421 aacgccgacg tctgcgatgc cagcatgctc agcaccgtca aggcaggcgc cgtggtctgc
      481 aacatcgggc acttcgacaa cgagatcgat accgccttca tgcgtgagca ctggcgctgg
      541 gagatggtca aggaccaggt gcatcaggtt ttccgaagcg acgatcgcca cgactacctg
      601 atcctgctgt ccgagggccg cctggtaaat ctgggcaacg ccatgggcca cccgtcgcgc
      661 gtcatggacg gctccttcgc caatcaggtg ctggctcaga tgcacctcta cgagcagcgc
      721 tgggccgacc agccggagaa ccagcgcgcg cccatcaccg tggaggtgct gccgaagaag
      781 ctggacgagg aggtcgccga gctcatggtc gccgggttcg gcggggtggt taccagactg
      841 acggccgagc aggcaaaata catcggtgtg gacgaaagcg gtccattcaa accggaatcg
      901 tacaagtact aggagacaga catgaccacc ccactttggt gcctgctgat cgtgatgctc
      961 atgcccatcg ttctggccgg cgtcggcggt tacttccgaa acaagcagct cggcacgctg
     1021 gacaacgacg acccccgaga gcagatcaag tcgttgacgg gtgccggctc aagggcctac
     1081 gccgcgcagc agaatgcctg ggaggccctg gccctgttca cggctgcggc gctcactgcc
     1141 cacgtcctgg gggcggaccc ggagagatcg gcccagctgg ccgtggcctt cgtggtgttc
     1201 cgggtgctgc atggggtctg ttacataggc ggctgggcga ccctgcgctc cctgatgttc
     1261 atgggctccc tggtcagcct gatctggctc ttcgttctgg gcgcctgacc cgtagcgccc
     1321 gggaacatag cgacagtgca gggcctcccg gaggtctcca tcggcgccct gctggtcgcc
     1381 ttcctgccga gtctggctct cctggcggtg atgcgcgcct ggtccctcca ggccgggcag
     1441 gcgctgattg ccaacatccg catgctcgtg cagctcctgg cgatcggcta cgtgctcgtg
     1501 ttcgtgttcg agaccgagaa cccgtggatc gtggccgccg tgctcgtgat gatgatgggc
     1561 gtcgctgcct ggatcgcgct gcgaccccta cagagccgca gcgcgcgcaa gtatctgcag
     1621 tccctgaccg ccatatccat ctgcggcctg gccatgctgg ccctggtgag ccagctggtc
     1681 atcgacctgc cccgctggtt cgagccacgc ttcgtggtgc cgctggccgg catgattttc
     1741 gccaacgcca tgaacacggt gagcctcgca gccgagcggt tcgagtccga gcaggcccgg
     1801 gaagtcgagt acgccgaggc acgcacgacg gccctggatg ccgcgctcat cccccaggtc
     1861 aacgccctgc tggcggtggg tctcgtgtct ctaccgggga tgatgacagg ccagatcctg
     1921 tccggcgtgg atccgctgat tgccgtgcgc taccagatcg tggtgatgtg catgattttc
     1981 gggtcttcag gattggcggc ggtcgcctac ctcttcgccc agcggccgcg gggggcctga
     2041 agcctgcgcc tcagtaacgc ccgccgagct tggcgtgatc ccagctgacg tatcgctccg
     2101 gcgtgaactt cagcagaacg cgtttctccg ccgtcttccc taccgcatca cgaatctggg
     2161 cgcgctccgc ctcggacacg tcgccacggc ccttggtgct gatgttgacc agcgcttcac
     2221 ggaccgcgtc gaactcctcg atcacctccg ctttggcata gatcagcacg ctcttgagct
     2281 ccgtatactc gtccccgctc tccacgagca gggacgcctt cgggtcgcgc cggaagttga
     2341 gcaccttctg ggccttgctg aacgtggtgc agtagaggca gccatccggg tcggtccaga
     2401 accacatggg cacagggtgg gcgtagccgt ccttgccgtt ggacacgatg atcagcgtct
     2461 tctgctcggc caggtagtcc ctgatctctc cctcggtcag ggcgatcagc tcgcggcgtg
     2521 acggcatccg ggcaactcct cggggcggcg gttaaagcgg cgatcgccat tctccccggc
     2581 gggtcggctg actacaatag cgctccctcc aagcccgcca gaagccgcca atgagccagg
     2641 accagaccgt caccgatctc atcgatctcc tcgatctgga ggagatcgag aagaaccact
     2701 accgcgcgac cagccccagc gagggttggc agcgggtcta tggggggcag gttctggggc
     2761 aggccctggt ggccgcctca cgcaccgtac ccgaggaccg tcacgcccac tccctgcacg
     2821 gctatttcct gcgcggcggc gacaccacgg tgcccatcct gtacaccgtc gaccggatcc
     2881 gggacgggcg cagcttcacc acccgtcgtg tggtagccat tcagcacggt aaggccatct
     2941 tcaacatggc catctccttt cagatcgacg agcccgggct gtctcatcag atgcccatgc
     3001 ccgaagtgcc agatccagag gggctgcccg acgagagcga gctgcgtaag gcatgggccg
     3061 acaagctccc cgagccgctg gaggcggcct tcgaccggga aaggcccatc gaggtgcgtc
     3121 ccatcgaccc ggtggacgtc ttcgcgccgg agaagcggcc gccacagcag atgtgctgga
     3181 tgaagtgccg cgaccgcctg cccgatgacc cgggcctgca ccagtgcgtg ctggcctacc
     3241 tttccgactg gtccctgctg gacaccgcca ccctccccca cggggtgagc ttcatgcagg
     3301 acaacatgca gatggcgagc ctggaccacg ccatgtggtt tcaccgtcct ttccgtgccg
     3361 acgagtggct gctctacgtt caggacagcc ccagcgccag cggcgcccgg ggcctcaacc
     3421 gtggcctgat ctacaaccgc gcaggagagc tggtggcgtc agccgcccag gaaggcctga
     3481 tccggcttca cgagaccgac tgatcggcga tggccaacgg atcgctcgcc agcgcggtgt
     3541 acaaggctga aggggagcag cgataatagc gggttttgtg cagcgcgaaa tacgcacgcc
     3601 caggtaagag ccgggctggg ggaagcagtg gccgaagcgg cagtgaacat cgaagagctc
     3661 accgagtccg tgggcagcct cagggacggc ctcgagtcac atcgctacat ctgcagcgac
     3721 cagatcgcca cggccgtcta cctcgcctac cacctgcaca aaccgatcct catcgaaggt
     3781 cctcccggcg tgggcaagac ggagctcgcc aagacgacgg cagagatgtt tgcgctgccg
     3841 ctgatccgtc tgcagtgcta cgaggggctc gacgaggcca aggccatcta cgagtggaag
     3901 tacggcaagc agctcctgta cacccaggtg ctcaaggaga cgctcgacga ggttctgcag
     3961 ggagcgaagg gctttgcgga atccgtgacg cggctgcacg agttcggcga catcttcttc
     4021 tccgaggagt ttttagagcc gcgcccgctg ctcaaggccc tgaaggagga agccggctgc
     4081 atcctgctca tcgacgaggt ggacaagtcc gatcacgagt tcgaatcgct cctgctggag
     4141 atgctgtccg atttccaggt gtccattccc gagatcggca cggtgaaggc cgtgcggcag
     4201 ccgcccatgg tgtttctcac cagcaacaac acccgggaga tctccgacgc gctcaagcgc
     4261 cgctgcctgc atctgtacat tccgtttcca gacgtcgatc tcgagcagcg catcatccac
     4321 gcccgggtcc cggagatccc gccggagctg agacgccagc tggtgcagtt cattcacgag
     4381 ctgcgcgatc tggacctgaa gaaggtgccg gccatcagcg agaccatcga ctgggcgcgc
     4441 acgctgctgc tcctgcacac cgagtcgctg gaccccaagc tggtccggga caccctcaac
     4501 gtgatcctca agttccagga ggacatcgac aacgtggacg cggaagtgac cacgctgacc
     4561 agcaaggcgc tcaaataagt ccggatggac cggaccctga ccaacttcat ccgcgcgctg
     4621 cgcaattccg aggtgcgcat ctccacggcg gagacgctcg acgccttcaa caccgtggag
     4681 ctcgtgggct atcgggatcg ggccgcgctc aagcgctcgc ttgcgctggt gctacccaag
     4741 acggcggacg agaaggagac gttcgagcag tgcttcgatg cgtatttctc cttcaccgac
     4801 atccaaggca gccagggagc ggccggcgag gctgagcgcg cctccgacgc cgagaccacc
     4861 gcaggcggcg aggaaggcgg tgaaggcggc ggcgctcagc ccggcgaagc gcgcccgggt
     4921 gatgccgctg gcagcgcgaa gaaaaaacag aagccgggcg caagcgccga cgcggaggca
     4981 gacgaggacg aggatctggg cgccggcgcg atgagcaagc cgcagtcgac cctcgggcag
     5041 ctgctcatgg ccaattccaa ggtcgagctg tccgtggcaa cggcggcggc cggagaggcc
     5101 gtcgacgtcc gcgacatcga ggtgttcact cagaaagggc tctacacccg ccggatcatg
     5161 gaggagatgg gccttgcgga tctgaaccgg gagatttccg agctgcgcca gcgcccggcc
     5221 gtgcctgatc gccgcctcgg ccaggagctc acccggcgcc gggactggct gcgcgagcaa
     5281 gtgcgcgact acgtggagaa acagtttctc cttcacgccg acgcgtcagg caagcggctt
     5341 cgcgaagagc tgctgcgcaa ggtaaagctg tccaacgtgg agcagcgcag cttccgcctc
     5401 atccaggaaa tcgtcttccg gatggccaag aagctggccg ctctgcattc gcgccgcaag
     5461 aaagtgttca agagggggca gatcaacatt ccgcgcaccc tgcgccacaa catggcctac
     5521 gacggagcca tcttcgacct gcgctggaaa tctgtgaaga tcgaccggcc caaggttttc
     5581 gcgatctgcg acgtctccgg ctctgtcgcc aactacgcgc gcttcatgct gatgttcctg
     5641 tacagcctgg aggaggtcat gcccaaggtg cgctccttcg ccttctcgtc ggatctggcc
     5701 gaggtgaccg agctcttcga gcgcaacgcc atcgaggacg ccatcgccaa gaccatccgc
     5761 gactacagtg gcggctcgac ggactacggc cagtcgctca tggacttcaa gaagctctgc
     5821 ctggacgagg tggacaaccg cacgaccatc atcatcctcg gcgatgcccg caacaattac
     5881 ggcgatccgc acacggagct cttaaaggag ctctacgatc gctgcaagcg gctgatctgg
     5941 ctgaatcccg agccgcgtaa cgcctggggc gtaggggact cggagatgcg ccggtacgcg
     6001 gcgtactgtc atcaggtaga agagtgcaac agcctcatgc atctcgagcg cattgtgggt
     6061 aatctgctgc gggtcgttca gtaggaagcg gagcgcggtg acaacggtcg caggaagcga
     6121 ggacaccttc aaccggtccg ccgggcggca gcttctcctg ttcgccgcca tcagcatcgg
     6181 cagcatcggg ctgctgatgt acgcgctggc ctgggcttcc agcctgaccg ggtccggcgc
     6241 tggctccgag gttgccgtgg atccggagac acgcacggtc acgctcttcc tgcgcgagga
     6301 acccccccag ctgaacagct ccctggccac cgacatggtc agcggcatgg tgctgggcca
     6361 cgtgatggaa ggcctgctgc gctacgacga gcacaaccgg ctggtgccgg gtatggccga
     6421 gcgctgggag atcgacgcca acggcgccat ctttcacctg cgcagcgacg cggtgtggag
     6481 cgacggtgcg cccgtcaccg cccacgactt cgtgttcgcc tggcagctgg ccctggaccc
     6541 cgccaacgcg tcccagtacg ccttcatcct ctacccggtg aagaacgggc gggccatcgc
     6601 cgaggccaag ctccccgttt cgcagctcgg cgcgcgggcc gtggacgacc gcaccctcga
     6661 ggtcacgttc gagcggccgg tggcctactt cgacaagctc gtggctttcc cgacttacta
     6721 cccgatccgc agggattttt acgaggcaac caagggtcgc tacggcgcgg atgcgcacac
     6781 gctgctctac aacggcccgt tccgcatgac gcgctgggtg cacggcgccc acgtgaggct
     6841 ggagaagaac cccacgtact gggatgagga acggatccgc atcgaggtgc tcgactttcc
     6901 gtacatcact tccgacgcca acgcgacgct gaatctcttc aaggacaaca aggtcgccta
     6961 cgccgggctg ctggcggaga atctcaacga cgccatggag cagcgctggc acatcaagcg
     7021 attcatggac ggctcggtct tcttcatcga gttcaacttt cgcgacgggc gcgtcaccgc
     7081 gaacacgaac ctgcgcaagg ccatgcagca cgccgtggac ccggcggagc tggtctacaa
     7141 ggtgaccaag ctgcccggct acatccccgg cgaatcgctg ttccccgtct ggctgcgcgg
     7201 cgtgaacggt tacttccgcc aggagtaccc ggcgccgaag acgatcaaag acgtggtcaa
     7261 ggccagagag tatctccagg cggcgaagcg ggagctgggg atggagcaga tccccccgct
     7321 ggtgctgcta agcggcgaca atcccgtctc caacattcag tccgagtact tccagcaggt
     7381 gttcaagcag accctcggtc tcgacatccg catagacaag cagatcttca agcagcgcct
     7441 ggccaagatg acttcgggcg atttcgacat ggtgctagcc ggctggggcc cggactacga
     7501 cgacccgctc accttcggcg acctgttcgc ctcctggaac ctcaacaacc ggggccgcta
     7561 cagcaacccc gagctggatc gccaggtagc catcgccgag gagtccctgg atcccaagac
     7621 gaggatggac gcgttcggcg agatacagcg gatcctgcac gacgacgtgg tgatcctgat
     7681 caactacgag cccggcacga cctacgtggt gaaccccgac ctcggcgggc tcgtgcgccg
     7741 ctccgtgggc ccggacccgg acttcaccaa cgcctacatc gtggaaggct gacgcttaag
     7801 acatgctcaa ctacacgctc aagcgtctgc tccagggcat cgtcaccgtc tggttcatag
     7861 ctaccgccac cttcatcgcc atgcacagcg tgcccggcga ccccttggcc gacgagaaag
     7921 ccgtcacggc agagatccgc gccaacctgg aagcaaaata cggcctcgac aagcctgttt
     7981 tcgaccagta cctgatctac atgggcaaca tcgtaaaagg agatttcggc atctccttca
     8041 ctcaggagaa ccgcgaggtc aacgacatca tccgtgagca cttccccgtc tccgccaccc
     8101 tgggcatcct cgcggtggtg ttcgccggcc tcggcggcgt gctgtggggc gccctgacgg
     8161 ccctgtaccg caatcggctg ccggacgtga tcatcatgtt cctggtgatc ctgggcattt
     8221 ccgtgccgag cttcgtcatc gccgccatca gccagctgag cctggtcaac ctcaacgacc
     8281 tgctgggcgt ctcgatactg cccgtggccg gctggggcag cgtgtggcac atgctggtgc
     8341 cgtccatggt cctcgggctc ggcaccatgg cttacctgac gcggctcatg cgctcctcca
     8401 tgctggaaat cgtcaacgcc gactacgtcc gcacggccaa gtccaagggg ctgccgccca
     8461 cgcgcatctt caccaggcac cagctgcgca acgcgatcct gcccgtgatc accgtgctgg
     8521 gcccgtccat cgccgccatc accacggggg gcttcgtggt ggagctcgtc ttcgccattc
     8581 ccggtctcgg ccgctacttc gtgcaggccg ttcagcagct cgactacacg gtgatcatgg
     8641 gcaccacggt attctacggc tcgttcctgg tcttcatggt catcgtggtg gacctgctct
     8701 acgggctcat cgacccgcgc gtgaggctcg agtagcccat ggccgagact gccctcaccg
     8761 cacgagactt cgagcccgtt cacgccgagc ggcggatcca gaccatctcc cggccgtccc
     8821 tgtcctactg gcaggacgcc tggatccgcc tgaaggccaa ccgccgcgca ctgttctcgc
     8881 tctacatcgt gctggggctg ctggccttca ccatcgccgg cccgttcgtg tggaccgtgg
     8941 acccggcggc ccaggacgtg gaccagatca gccagccacc gggcacggac cggcgtgccg
     9001 ccgtggtgga gccctaccag ccctgggacg gctcatcggt gctgccgctc acggaaggtc
     9061 ttcgactcgc cgagccggcc accaaccagg ccgtgcggct gatgtgggac gcaacgcccg
     9121 gtgctgccgg ctacgtcatc taccggaacc tcttccccgt cggcccggag aatgccctgg
     9181 ggctgccgct gggcgaaaac ttctccgccg aagacacgaa gttcgaggat ctgctggatc
     9241 ttcgcccgcg ccgctactac tacagcgtcg tcagcctggg cgccgccggc aacgagctgg
     9301 ctcaggagac gctgatggtc aacgtcgacc gggtcatcac cgtagcggaa gccgaggccc
     9361 ggggcatggg tgagggccgc cagctggccg tcggcgacat cgtgaggctg cccctgcatc
     9421 ctctgggcac ggactatctg ggccgcgacc tcctggcccg gctcatggag ggcgcgaggg
     9481 tgtccctctt catcggcatc tgcgcgccgt tcctctacgt gctgttcggg gtggcctacg
     9541 gcagcgcggc gggctttctc ggcgggcact tcgatcaggc gctcatgcgc ttcgccgact
     9601 tcgtcatcgc cctgcccttc ctgctgttca tgatcctgtt caagatcgcc ttcggcatcg
     9661 gcccgggcga gagcggcatc atgcccatgc tggcggccat ggtcctgctc tcctggccgg
     9721 gcacgtcgcg cctggtgcgc gggcagatcc tgcagatccg ggaggagggc tacatcagcg
     9781 cctcgcgcct gctgggcgcg aagaccggct acctggttct gcgccacatg attcccaaca
     9841 ccatgggcgt catcctggtc acgctgacct tcgcggtgcc cagcgccatc ttcacggagg
     9901 cgttcctgtc cttcatcggc atgggcgtcg cccccccgac gccgtcctgg ggcagcatgt
     9961 gcaacgaggg catccgcacc atgctgacca cgccccacga gctgatcttc cccgcgctct
    10021 tcatcagcgt gacggtgctg gccttcaacc tgctcggaga cggcctgcgc gatgcccttg
    10081 acgcccgcat gaggtccacg gagtgagcac gccgcggctt ctggaggtgg aggatctgaa
    10141 ggtggagttc gacacctacg gcggcgtggt gcaggccgtc cgcggggtga gcttcggggt
    10201 ggacgagggc gagacgctcg ccatcgtcgg cgagtccggc tgcggcaagt cggtgacggt
    10261 gcagagcatc atgggcctga cccccatgcc gcccggccgc atcacctcgg gcagcgcccg
    10321 gctgcgcggc acggacatca tcgggcgcaa ggagatcggc ggcgaggaca tccgcggcgg
    10381 ccagatcggc atgatcttcc aggaccccat gacgtccctg aaccccacca tgaacatcgg
    10441 ctcccagatt gcggagaccc tggaggttca ccggggctac tccaagaaac aggcattcca
    10501 gcgggccgtg gagctcctgg acatgagccg gatccccgag gcggccagcc gggcgcgcca
    10561 gtacccgttc gagttctccg gcggcatgct gcagcgggcg atgatcgcca tggccatcgc
    10621 ctgcgagccg gccatcctca ttgccgatga gcccaccacc gccctggacg tgaccatcca
    10681 ggcgcagatt ctcgatctca tgctcgacct gcagcgcgag accggcatgg ccatcatcct
    10741 catcacccac gatctggggg tggtggcgcg gatggcggac gaggtggcgg tgatgtacgc
    10801 gggccagatc gccgagcaag gcaccgtgga cgacgtcttc taccgctcct cccaccccta
    10861 caccctgggg ctccgggcag ccatgcccaa caacgacccg gcccgccagc acgagctgaa
    10921 gcccatcgac ggcagcccgc ccgacctctt ccacccgcca aagggatgcg cctacgccgc
    10981 gcgctgcccc tacgccatgc gcatctgcga aagcgaggat ccgcacgcct accacatgac
    11041 cgagcgccac ttcgcccgct gctggcagca ccacgaaggc gccgccccgc gcgtcgaggg
    11101 cctgtactac cgggaggcgc gggcgtgagc gaacggccgg tgcttctcga gacggaccat
    11161 ctcaccaagc atttcctgat cgggcgagga caggaggtgc acgccgtgga cggcgtgagc
    11221 ctggccattc acgagcagga gatcgtcggg ctcgtcggcg agagcggatc cggcaagtcc
    11281 acctacggga agaccgtggt gggccttcac gacaagacca gcggcaccgt cacctaccgg
    11341 ggcgaacggc taccccagcg ctacggcgcg gccgatttcc agaagcacgc ccgccacatg
    11401 cagatgatct tccaggaccc ctactcgtcg ctgaacccgc gcatgacggt gggcgagatc
    11461 atcggcgagg gcttgcggct ccacgccaac ctggacgttc gcgaagtgcg cgagcgggtg
    11521 gccgagtggc tgaagcgcgt cgggctcgag cccgaccaca tgtcccgcta cccccacgag
    11581 ttctccggcg gccagcgcca gcgcatcggc gtcgcccggg cactgatcct ggaacccgag
    11641 ttcgtcgtct gcgacgagcc catctctgcg ctcgacgtct cggtgcaggc gcaggtcatc
    11701 aacctgctcg acgacctgaa gcgctccatg gggctcacca tgctcttcat cgcccacgat
    11761 ctgtccatgg tgcgctacgt gagcgaccgc atggcggtga tgtacctggg ctccgtggtg
    11821 gagatcggcc ccgccaacga cgttttcttc aacccgaagc atccgtacac ggagatcctc
    11881 gttggctcca acccggagcc ggacccggag gccgaacgcc atcgggcgtc aacggccatc
    11941 gtcggcgaga tcccttcacc ggtgaacgtc cccgaggggt gccgcttcgc caaccgctgc
    12001 ccgaaagtga tggacgtgtg ccgaagcgtc acgccggagc tgatcccgtt aaagaacgcg
    12061 gaagccgtcc gtgaagtggc ctgtcacctg tactgatagc cgcctatgga cgccatcttc
    12121 atgatgcaag ggccatcaac atccgaggca agcaaaggcc cgcacaagtt gcgggctcga
    12181 atcgcgggcc gatgcagcgg gcgctgggca cgatcggctt tcgtaagcgg cggacataaa
    12241 aaaaccccgc tcaggccctt gagcccggcg gggtttcgga tcagcgggcg cccgtcatcc
    12301 aggcaccctg aagatcgtcc ctaccagttg aacccgacgg tcagaccgaa ggtctgcgga
    12361 tccagcagga actggttggt gaacagccct gacacctgcg tggacaggta ctgctgcacc
    12421 acgtagtcgt tgtcgaggat gttcttgatc caggcttccg cgtaccagcg ctcgtcgttg
    12481 ggcgcgaacc gagcccgcgc attccacacg tcccaggact ccaccttgtc gtgcagggca
    12541 ttgttgttgc gcacgtagaa ggagtcctgc cagtagaaat tggtggccac ggtcaggccc
    12601 agcccgccca tgggcacgcg gtagcgggcg ccgatgttga agctgaactc gggcgactgg
    12661 gcgagctgat tgccatcctg gctctgcaga acacccggaa tccggcccgg accgcagccg
    12721 ttggggctcg tgtccaggaa cacgctggca agacagacga tcaatccatc cgtgctgcca
    12781 tctcctttcg ggtcggccgt gtcgtaatcc tcgaagtcct tgatctcgga atcgagccaa
    12841 gagcccgtca aatcgaactg caggttctcg ataggcgccc agagcacctc gacctcggcc
    12901 ccgaggatct ttgcgtcggt gtttttgttg agcgaggtga cgtcgacgat cttggacacc
    12961 tgaaggtctt tgtaatcata gtaaaagaat gagccgttca gctgcatggt cctgtccagc
    13021 agcgtcgact tgaagccaac ctcgaaggca tcaatggtct cgggctcgaa ggtggcgttg
    13081 gagggatcgt cgataaccag ctggtcgctg tcgctgatgg ggttgaagcc gccgctcttg
    13141 tagctggacg acacggttcc atacaccatc aggtcgtcgt tgacgtcata ggtgagattg
    13201 accttccagg tgacctcgtt gtcgtcgtat ttcgggaaga agtagccgtt ctcgccctcc
    13261 gggtcgaagg gcccgcactg caggaacgtg acgtagcacg tccgctgctt ggagctcttc
    13321 ttctcgtcgg agtaacgcag accgatggtc gagctcaggc tgtcggtgat gtcccagtag
    13381 agctcaccga agatggccca gctctccagc t
//
LOCUS       DBNHKV010000423         8214 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_626388, whole genome shotgun sequence.
ACCESSION   DBNHKV010000423 DBNHKV010000000
VERSION     DBNHKV010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_626388"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1475
                     /gene="glmM"
                     /locus_tag="AC16PP_13780"
     CDS             141..1475
                     /gene="glmM"
                     /locus_tag="AC16PP_13780"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003221540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR6482746.1"
                     /translation="MEKKYFGTDGIRGRVGDAPITPEFCLRLGWAAGRVFARSGKCHV
                     LIGKDTRISGYMLESVLESGLVSAGANVSLLTVMPTPAVAYLTRTLRADAGIVISASH
                     NPYCDNGIKFFDGSGNKLPDAVELEIEAELNREMACVDSDRLGKADRLVDAPGRYIEF
                     CKASVDRALRLKGLKIVLDCAHGATYHVAPRVFEELGAEVSVCGANPNGTNINDNCGA
                     TAPARLAERVAEEGAHLGIAFDGDGDRVIMVDEQGDIVDGDELLYVIAKHRATQGTLH
                     GGIVGTLMTNYGLERALAGLNVPFERAAVGDRYVLEMLRERGWTLGGEASGHIVCLDL
                     TTTGDAIVAALQVLTAIVQSGQPLHQLKQGMSKLPQVMVNVSAPDPSRAADERALQLA
                     VRDKSRLLAGRGRILVRRSGTEPVIRVMVEGEDHAEVSAIADDLAAAVQQEG"
     gene            1565..2326
                     /gene="tpiA"
                     /locus_tag="AC16PP_13785"
     CDS             1565..2326
                     /gene="tpiA"
                     /locus_tag="AC16PP_13785"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008017141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR6482747.1"
                     /translation="MSPRRPLVAGNWKMNGSSEFVRSFAAELMARVVEDAVSVVVFPP
                     AAYLGALVRAFEGSAIEVGGQNAHMESSGAFTGEVAPEMVADVGGRWLLVGHSERRQY
                     FGESDDLIADKYAAALRACLAPVLCVGETLEEREAGEAERVVCRQLAAVTERLDIAEV
                     ARGVVAYEPVWAIGTGRTATPGQAQEMHRVIREALSERGAKLAEGMRILYGGSVNADN
                     AGELMAQPDIDGGLVGGASLSADGFAAIVAAAAGR"
     gene            2412..2741
                     /gene="secG"
                     /locus_tag="AC16PP_13790"
     CDS             2412..2741
                     /gene="secG"
                     /locus_tag="AC16PP_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR6482748.1"
                     /translation="MLLVVDAIALAALVLLQQGRGADVGAAFGSGSSNTMFGSAGSAS
                     FLTKLTVWLAIGFFLISFGLAYTAKERAAIIDEVGVPSVAPAEETVVPEQLTPEPESV
                     DSDIPDV"
     gene            2750..2836
                     /locus_tag="AC16PP_13795"
     tRNA            2750..2836
                     /locus_tag="AC16PP_13795"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2784..2786,aa:Leu,seq:gag)
     gene            2956..3032
                     /locus_tag="AC16PP_13800"
     tRNA            2956..3032
                     /locus_tag="AC16PP_13800"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2990..2992,aa:Met,seq:cat)
     gene            3294..3764
                     /gene="rimP"
                     /locus_tag="AC16PP_13805"
     CDS             3294..3764
                     /gene="rimP"
                     /locus_tag="AC16PP_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR6482749.1"
                     /translation="MNAKEKQIEALLTPTVEGLGCQIWGVEYASQGRHSRLRVYIDRE
                     EGVSVDDCERVSRHVSDVLDVEEVISGGYTLEVSSPGMDRVLFKAEQYAANVGQHVDV
                     RLVYPFEGRKRIVGVLAGLEEDDSCGHVIVQVDDEQYLLPLESVQRTRIIPQYD"
     gene            3780..5297
                     /gene="nusA"
                     /locus_tag="AC16PP_13810"
     CDS             3780..5297
                     /gene="nusA"
                     /locus_tag="AC16PP_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR6482750.1"
                     /translation="MSKEMLLVVDTVSNEKGVPKDVLFGAVEAALATATKKRYDDDAE
                     FRVAIDRESGEYETFRVWTVADPEALEEEEEWNPAAQLTVEEARERDANLQLGDVIEE
                     PVESVGFGRIAAQTAKQVIVQKVREAERAQVVEAYLPRVGELVNGTVKKLGRDSVIVD
                     LGNNAEGLLTREHLIPRETFRVGDRLRALLLEVNPDNRGPQLMLTRTSPKMLIELFKL
                     EVPEIAEDVIEIRGAARDPGSRAKIAVKTNDGRIDPVGACVGMRGSRVQAVSGELAGE
                     RIDIVLWDDNPAQLTVNAMAPAEVAAIVLDEDTHAMDIAVTEDNLAQAIGRGGQNVRL
                     ASDLVGWSLNIMTEEDAAAKQEEEAVRYIEEFMRALEVDEDVATVLVEEGFTSIDEVA
                     YVPIEEMTAIEGFDEEIVEELRNRAKDALLTKAIASEELLGEKEPADDLLNMEGMERR
                     LAFQLASNDIITMEDLAELSVPDFIEIVPEVDEETAAALIMTARAPWFEEAEAEG"
     gene            5323..7857
                     /gene="infB"
                     /locus_tag="AC16PP_13815"
     CDS             5323..7857
                     /gene="infB"
                     /locus_tag="AC16PP_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR6482751.1"
                     /translation="MSEVTVKQLADTVGAPVDRLLKQMQDAGLPHDSDDAAVTEEEKQ
                     TLLAYLKRSHGSDDAAPKKITLKRKTVGTLKSGQGRTGRTVNIEVRRKRTYVKRDEAE
                     TAGAAAEAPAKPASQSELEAQRIREQELARKEAEEETRRKEAERKAEEEKRVEEIKRQ
                     EEADLQARETKPGEAPAEDSAAKAAAAAAEAQAKAQASERGGKRVRGKERERPGEKRD
                     EFGDGKGRRRELSLKTQRRGKRRQPAPMPVEQQGGEFRPTDFIAREVEIPDIITVGEL
                     AQRMTVKAGEVIKALMGMGVMATINQSVDQDTATLIVEELGHKPKPIHEDAMEQALEA
                     SLQIEGDAAKRAPVVTVMGHVDHGKTSLLDYIRKTRVTSGEAGGITQHIGAYSVDTPH
                     GKISFIDTPGHAAFTAMRARGARVTDIVILVVAADDGVMPQTEEAIQHAKAAGVPVVV
                     AINKMDLEGADPDRVTNELAAKEVVPEDWGGDTQFVKVSAVSGEGIDELLEALLLQAE
                     ILELTAVEDAPGRGVVIESRLDRGRGPVSTVLVQNGTLKQGDVIIAGECHGRVRAMLN
                     DKGEQVKAAGPSVPVEVLGINGTPAAGDDFSVATDERTARELAEFRRDKSQEQRVATQ
                     HAAKLENMFANVGAGEKRVLKMVLKADVRGSLEAITQALTEMGNEEVAVNVLGSGVGG
                     ITETDANMALTYGAAVFGFNVRADNSAKAIIEREGLDLRYYSVIYELLDDVKQVLSGM
                     LSPEVREEIVGIAEVRDVFRSPRFGQVAGCMVTEGTVYRNKPIRVLRDNVVIYEGELE
                     SLRRFKDDVNEVRSGTECGIGVRNYNDVRVGDLIEVFDTKEVAREL"
     gene            7896..>8214
                     /gene="rbfA"
                     /locus_tag="AC16PP_13820"
     CDS             7896..>8214
                     /gene="rbfA"
                     /locus_tag="AC16PP_13820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR6482752.1"
                     /translation="MREFGRDLRVADFVRDELAKIIQREMRDPRVGMVSVNEVTVSRD
                     LAFADVYVSSIEARTAKEREELIGVLNKAAGFFRSELAKRHSMRTTPKPRFHYDEMVE
                     RGPE"
BASE COUNT         1600 a         2334 c         2887 g         1393 t
ORIGIN      
        1 gttgagcacg gcgccagcgt ggtgaggacc cacgacgtcg gcctcaccgc cgatgcgctg
       61 aagctggtga cggcctggca cgggcacgga tgaagaccgg gccttggtct ggctttggtt
      121 ttggtaagga tgcgcttggg atggaaaaaa agtacttcgg gactgacggc attcgtggtc
      181 gggttggcga cgctcccata acacccgagt tctgtctgcg tcttggctgg gcggcgggca
      241 gggtgttcgc gcgcagcggc aagtgccacg tgctcatcgg caaggacacg cgcatatcgg
      301 ggtacatgct cgagtccgtt ctggagtccg gtctcgtttc cgccggggcc aacgtcagcc
      361 tgctcaccgt catgcctacg ccagccgttg cgtatctgac gcgcacgttg cgggccgacg
      421 cgggcatcgt catcagcgct tcgcacaatc cctactgcga caacggcatc aaatttttcg
      481 acgggtcggg aaacaagctg ccggatgccg ttgagctaga gatagaagcc gaactgaatc
      541 gcgaaatggc gtgcgtggac tcggatcgcc tcggcaaggc ggaccggctc gtcgatgcgc
      601 cggggcgcta tatcgagttc tgcaaagcca gcgttgaccg ggcgctgcgc ctgaaaggcc
      661 tcaagatcgt gctcgactgt gctcacggcg cgacctatca cgtcgctccc cgcgtgttcg
      721 aggagctggg cgcggaggtc agcgtgtgcg gggcgaaccc taacggcacg aacatcaacg
      781 acaactgcgg cgccacggct cccgcccggc tcgccgagcg ggtggcggag gagggcgccc
      841 atctgggaat cgccttcgac ggagacggcg accgggtgat catggtcgac gagcaggggg
      901 acatcgtcga tggagacgag ttgctgtatg tgattgccaa gcaccgggcg acgcagggga
      961 cccttcatgg gggcatcgtc ggaaccctga tgaccaatta cggcctcgag cgcgcgctgg
     1021 ccgggctcaa cgtgccgttc gaacgggccg ccgtaggcga ccggtacgtg ctggagatgc
     1081 tgcgcgagcg cggctggacc cttggcggtg aagcgtcggg acacatcgtg tgtctggatc
     1141 tcacgaccac gggcgatgcc atcgtggcag ccctccaggt cctgaccgcc atcgtccaga
     1201 gcgggcagcc gctgcaccag ctcaagcagg gcatgtccaa gctgccccag gtcatggtca
     1261 acgtttctgc acccgacccg tcccgtgcgg cggatgagcg ggcgctgcag ctggcggtca
     1321 gggacaagag ccgcctgctc gccgggcgtg gccggattct cgttcggcgt tcggggacgg
     1381 agcccgtcat ccgggtcatg gtcgaaggcg aagatcacgc cgaggtctct gccattgcgg
     1441 acgatcttgc cgccgcggtc cagcaggaag gctgaagcct tgcaagctgc tggcgcgccg
     1501 ggtcgcggcg ttgtctgcgt cagccccaaa gtataagatt gcgcgccctt aggtggaggc
     1561 cggcatgtcc ccacgtaggc cgctggtcgc gggcaactgg aaaatgaacg gctcttccga
     1621 gttcgtccgg agtttcgcgg ctgaactcat ggcccgggtc gtggaagatg cggtgagcgt
     1681 agtcgtgttc cctcccgcgg cctatctcgg cgcgctggtc cgggccttcg aagggtctgc
     1741 catagaggtc ggtgggcaga atgcacacat ggagtcctcg ggggctttca ccggcgaggt
     1801 cgccccggaa atggtggccg acgtcggggg tcgctggctg ttggtcggtc attccgagcg
     1861 gcgccagtat ttcggcgagt cggacgacct gatcgctgac aagtatgccg ccgcgttgcg
     1921 agcgtgcctt gccccggtgc tatgcgtggg cgagacgctc gaggaacgcg aagccgggga
     1981 ggccgagcgg gtcgtctgca ggcagctcgc cgccgtcacg gagcgattgg acatcgccga
     2041 ggtggcccgg ggcgtagtgg cttacgagcc ggtatgggcc atcggcacgg gtcgcacggc
     2101 gacgcctggc caggcccagg agatgcatcg ggtcattcgc gaagcgttga gcgagcgcgg
     2161 tgcaaagctg gcggaaggga tgcggattct ctacggtggc agcgtaaacg ccgataatgc
     2221 cggcgagctg atggcccagc cggatatcga cggcgggctg gtcggaggcg catcgctcag
     2281 cgcggacggg ttcgcggcga tcgttgctgc ggcggctgga aggtgacgga acagtgggcc
     2341 tgctccgggg caggcggtga gcagaaaaat cggtaacgtg ccacatgaca acttttgaaa
     2401 cgattctgtt gatgctgctc gttgtggacg ccatcgcgct ggcagcgctg gtgctgctgc
     2461 agcagggccg tggcgcggac gtaggcgcgg cattcggcag cgggtcctcc aacaccatgt
     2521 ttggcagtgc cgggtcggct tcgttcctga cgaaactgac ggtgtggctg gccataggct
     2581 ttttcctgat ttctttcggg ctcgcctaca ccgccaagga gcgcgcggcc attatcgacg
     2641 aggtcggggt tcccagcgtg gctcctgctg aggaaactgt cgtcccggag cagttgactc
     2701 cggaaccgga aagtgtagat tccgacatcc ccgatgtttg aggggttctg ccgaagtggt
     2761 gaaactggta gacacgctac cttgaggggg tagtgggcgt acgcccgtgg aggttcgagt
     2821 cctctcttcg gcaccattcc cgttccagct cggcccggca aaaatgtcgt ttaaaaccgc
     2881 gatgttgcgt ggtgcggccc gatatttacc ggttgcgggg ggcatgagcc atcaatataa
     2941 tgcgccactt gctgatgcgg ggtggagcag tctggtagct cgtcgggctc ataacccgaa
     3001 ggtcgtgggt tcaaatcctg cccccgctac caaaggataa aagcccctcc tgtgctttgc
     3061 acgaaaaggt caggggcttt ttattacgcc ggggggcgcg tcttcccggc gtaggcgcag
     3121 cgggccttat gaacccgcca tgacatcctt gtaggtggcc cgccgcgtac cggctgggcc
     3181 gcccgtggtc tgatgcgaaa gaagcgatct cgcgcaattg catcgggtat gagaatgggc
     3241 gtaagcccat tttttattgg ctgaaggaaa gcctgacggc gtttgacaag ctgttgaatg
     3301 cgaaggagaa acagatcgaa gcgttgctga cgcccacggt agaagggctg ggctgccaga
     3361 tctggggggt cgagtacgcc tcccagggtc ggcattcgcg tctgcgtgtc tacatcgacc
     3421 gcgaggaagg cgtttcggtg gacgactgcg agcgtgtgag ccgacatgtc agcgacgttc
     3481 tggatgtgga agaggtgata tccggcggct atacgctgga ggtgtcctct ccgggcatgg
     3541 atcgggtcct gttcaaggcg gagcagtacg cggcgaacgt cggccagcac gtggatgtcc
     3601 gtctcgtata tccgttcgag gggcgcaaac ggatcgtagg ggtgttggca ggcctggagg
     3661 aagacgattc ctgcggacac gtaatcgtgc aggtggatga tgaacagtac ctgttgccgc
     3721 tggagagcgt tcagcgcacc aggattattc cccagtatga ctgaggtgcg accaaagtta
     3781 tgagcaaaga gatgttgttg gtggtggata ccgtctccaa cgagaagggc gtcccgaagg
     3841 acgtgctttt cggcgccgtg gaggcagcgc tcgcgactgc caccaagaag cgctacgacg
     3901 acgacgccga gttccgcgtc gccatcgacc gggagtctgg agagtacgag acgttcagag
     3961 tctggaccgt ggcggatccg gaggcgctgg aagaagagga agagtggaac cccgcggctc
     4021 agctgaccgt ggaggaagcc cgggaacggg atgcgaatct tcagctcggc gatgtgatcg
     4081 aggagccggt ggagtcggtg ggctttggcc gtatcgctgc gcagacggcc aagcaggtca
     4141 tcgtgcagaa ggtccgtgag gccgagcgtg ctcaggtcgt cgaagcttac ctgccgcggg
     4201 tcggggagct cgttaacgga accgtgaaga aactcgggcg tgacagcgtc atcgtagatc
     4261 tcggcaacaa cgccgagggc ctcctgaccc gggaacacct gattccccgg gaaacgtttc
     4321 gtgtgggtga ccggttgcgc gcgcttctgc tcgaggtcaa tccggacaat cgcggacctc
     4381 agctcatgct gacgcgcacc tcgccaaaga tgctcatcga gcttttcaag ctggaggttc
     4441 ccgagatcgc cgaggacgtc atcgagatac ggggcgcggc gcgggatccg gggtccaggg
     4501 cgaagatcgc ggtgaagacc aacgacggtc ggatcgatcc ggtaggcgcc tgcgtcggca
     4561 tgcggggctc ccgcgtgcag gccgtatccg gtgagctggc cggggagcgg atagacatcg
     4621 ttctttggga cgacaacccg gcccagctta ccgtcaacgc catggcgccg gccgaggtgg
     4681 ccgccatcgt gctcgatgag gacacccacg ccatggatat cgcggtcacc gaggacaacc
     4741 tcgctcaggc gatcggccgc ggcgggcaga atgtgcgtct ggccagtgat ctggtcggct
     4801 ggtcgctgaa catcatgacg gaagaggatg cggcggcgaa acaggaagag gaggccgtgc
     4861 gctacattga ggagttcatg cgcgccctcg aggtcgacga ggacgtcgcc accgtgctgg
     4921 tggaagaagg tttcacctcc atcgacgagg tcgcctacgt gcccatcgag gagatgacgg
     4981 ccattgaagg gttcgacgag gagatcgtcg aggagctgcg caaccgggcc aaggatgctc
     5041 tgctgacgaa ggcgatcgcc agtgaggagc tgctcggcga aaaggagcct gcggacgatc
     5101 tgctgaacat ggaaggcatg gagcgacgcc tggcgttcca gctggccagc aatgacatca
     5161 tcacgatgga agatcttgct gaactttcgg tgcccgattt catcgagatc gtcccggagg
     5221 tcgacgaaga gaccgcggct gctctcatca tgacggcccg cgcgccctgg ttcgaagaag
     5281 cggaggcgga agggtaagcc gccccgaaga ggaaaaagag tcatgtcgga agtcacagtc
     5341 aagcaactcg cagatacggt gggggcgccc gtagaccgtc tgctcaagca gatgcaggat
     5401 gccgggcttc cacacgacag cgacgacgcg gcggtcacgg aagaggagaa gcagacgctg
     5461 ctcgcctacc tcaagcgcag ccacggatct gacgacgccg cgccaaagaa gatcacgctg
     5521 aagcgaaaga ccgtcggaac cctcaagtcc ggccagggca ggacgggccg taccgtgaac
     5581 atcgaggtgc gccgtaagcg cacctacgtg aagcgggacg aggccgagac cgcgggcgcg
     5641 gcggcagaag cgccggcaaa gccggccagc cagtctgagc tcgaggcgca gcgtattcgg
     5701 gagcaggaac tcgctcgcaa agaggccgaa gaggaaaccc ggcgcaagga agcggagcgc
     5761 aaggccgaag aggaaaagcg ggtagaggag atcaagcgtc aggaggaagc cgaccttcag
     5821 gcgcgcgaaa ccaaacccgg cgaggctccg gcagaagact cagcggcgaa agcggccgct
     5881 gccgctgcag aggcccaggc gaaggcccag gcaagcgagc gcggcgggaa acgcgtacgc
     5941 ggcaaggagc gcgagcggcc gggcgaaaaa cgcgacgagt tcggcgacgg caaaggccgc
     6001 cgccgggagc tctcgctcaa aacgcagcgg cgtggcaaac gtcgtcagcc cgcaccgatg
     6061 cccgtcgagc agcagggcgg ggaattccga ccgacggact tcatcgcgcg ggaggtcgag
     6121 atacccgaca tcatcacggt gggcgagctg gcccagcgca tgacggtgaa ggcaggcgaa
     6181 gtcataaaag cgctcatggg tatgggcgtg atggcgacga tcaaccagag cgtcgatcag
     6241 gataccgcca cgctcatcgt cgaggagctg ggccacaagc cgaaacccat ccacgaggat
     6301 gccatggagc aggctctgga agcctcgctg cagatcgagg gcgatgccgc gaagcgggca
     6361 cccgtggtga cggtcatggg gcacgtcgac catggcaaga cctcgctgct ggactacatc
     6421 cgcaagactc gggtcaccag cggcgaagct ggtgggatca cccagcacat cggcgcctac
     6481 agcgtggaca cgccgcacgg caagatcagc ttcatcgata cgcccggtca tgccgccttt
     6541 accgccatgc gggctcgtgg cgccagggtt acggacatcg tcatcctggt cgtggctgcg
     6601 gacgacggcg tcatgcccca gaccgaggaa gcaatacagc acgccaaagc ggccggcgtg
     6661 cctgtggtcg tggccatcaa caagatggac ctcgaaggcg ccgacccgga ccgggtcacg
     6721 aacgagctgg cggccaagga agtggttccc gaggattggg gcggcgacac tcagttcgtc
     6781 aaggtctcgg cggtctccgg cgaaggcatt gacgagcttc tcgaggcgtt gctcctgcag
     6841 gcggaaatcc tggagctgac ggccgtggag gatgctcccg ggcgcggcgt cgtcatcgaa
     6901 tcgcggctcg atcgcggtcg ggggccggtg tccaccgtcc tggtgcagaa cggcaccctc
     6961 aagcaggggg acgtgatcat tgcgggcgaa tgccacggcc gcgttcgtgc catgctcaat
     7021 gacaagggtg agcaggtgaa agcggcgggg ccgtcggttc ccgtcgaggt gctcggcatc
     7081 aacgggaccc cggcagccgg tgatgatttc tcggttgcaa ccgacgagcg caccgcgcgg
     7141 gagctcgccg agttccggcg ggacaagagc caggaacagc gtgttgcgac gcagcacgct
     7201 gccaagctcg aaaacatgtt tgccaacgtg ggagccggag agaagcgcgt tctcaagatg
     7261 gtcctgaaag ccgacgtgcg cggcagcctc gaagccatta cccaggccct caccgaaatg
     7321 ggtaacgagg aggtggctgt caacgttctg ggatccggtg tcggtggcat caccgaaacc
     7381 gacgcgaaca tggcgctcac ctacggcgcg gcggtcttcg gattcaatgt tcgagcagac
     7441 aactctgcta aggccatcat cgagcgggaa gggctggatc tgcgctacta cagcgtgatc
     7501 tacgagcttc tcgacgacgt caagcaggtg ctttccggca tgctctcgcc ggaggtgcgc
     7561 gaggagatcg tggggatcgc cgaggttcgg gacgtgttcc gttcaccgcg gtttggtcag
     7621 gtcgccggct gcatggtcac cgaaggtacg gtgtatcgca acaaacccat tcgcgtcctg
     7681 cgcgacaacg tcgtgatcta cgaaggggag ctggaatcgc tgcggcgctt caaggatgac
     7741 gtcaacgagg ttcgcagcgg tacggaatgt ggcatcggcg ttcgtaacta caacgatgtt
     7801 cgcgttggtg atctcatcga ggtcttcgat acgaaagaag tggcgcggga gctctaggat
     7861 gcgccgggta attgagcctg tgtgggccgg gatgcatgcg tgagtttgga cgtgatctgc
     7921 gggttgccga tttcgtgcgc gacgagctcg ctaagatcat ccagcgtgag atgcgggacc
     7981 cccgcgtggg catggtcagc gtgaacgaag tcactgtcag cagagacctg gcgttcgcgg
     8041 acgtttatgt gtcctccata gaagcacgga ccgccaagga acgcgaagag ctgatcggcg
     8101 tcctcaacaa ggcagcaggt ttttttcgct cggagctggc caagcgtcac agtatgcgga
     8161 cgacgccgaa gccccgattc cactacgacg agatggtcga aagaggtccg gagc
//
LOCUS       DBNHKV010000424         4771 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_201193, whole genome shotgun sequence.
ACCESSION   DBNHKV010000424 DBNHKV010000000
VERSION     DBNHKV010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_201193"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="AC16PP_13825"
     CDS             complement(<1..525)
                     /locus_tag="AC16PP_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6482753.1"
                     /translation="MIFDSTDTYISTDELSMAVDAAVKLERPLLVKGEPGTGKTMLAE
                     EIAKSLGLRLIQWHIKSSTKAINGLYEYDAVSRLRDSQLGDERVQDIRNYIKTGKLWE
                     AFEADERVVLLIDEIDKADIEFPNDLLQELDRMEFFVYELGETIKAKQRPVVVITSNN
                     EKELPDAFLRRCFFH"
     gene            complement(776..1138)
                     /locus_tag="AC16PP_13830"
     CDS             complement(776..1138)
                     /locus_tag="AC16PP_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482754.1"
                     /translation="MGNAWVWAAIVVGGLGAAAGLHIALSGLLAREIRLAVVFVFLCL
                     LNTPAPVPEYAGSYAPALIILAFEGLFQRGGDPFTALKILLAVAALASGAAVLMVLGW
                     RRWASTSLPNAESADSSL"
     gene            complement(1138..1578)
                     /locus_tag="AC16PP_13835"
     CDS             complement(1138..1578)
                     /locus_tag="AC16PP_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HIR6482755.1"
                     /translation="MDRFWEHKTLGELSAEEWESLCDGCARCCLVKLEEEGSGCVHYT
                     GLVCDLLDLETCRCTRYPDRHRLVKDCIDFSAEVMASLSWLPRSCAYRRVAEGRGLAD
                     WHPLVSGSPDSVHRAGISVLGRVIPAGSVHEDEFEEHLIQWVET"
     gene            complement(1578..1829)
                     /locus_tag="AC16PP_13840"
     CDS             complement(1578..1829)
                     /locus_tag="AC16PP_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017526690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HIR6482756.1"
                     /translation="MSRLVQIYRSSRRNGMYLYVDAGEDLARVPEALMKSFGRPVAAM
                     SLALTRDRQLARADAAAVLKNIEECGFYLQMPPSVDELS"
     gene            complement(1826..2938)
                     /locus_tag="AC16PP_13845"
     CDS             complement(1826..2938)
                     /locus_tag="AC16PP_13845"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:NF013755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HIR6482757.1"
                     /translation="MPCRADAASVSWIDDTGALVDAVASWGDVIGLDTEFQRTDTFFP
                     VPGLYQVISGEDVYFLDPLSIDDFSAFADVLHDPAIVKVMHACSEDLELIRHHLGVEP
                     QGIFDTQLAYAFQNSRYSVSYANLVAALLGHDLGKHATRSNWLRRPLTEEQLHYAWED
                     VRYLPEVYEALTARLDELGRRSWFADAMAEKGRVQGAHPDEYYRGVKKAWRLDALELG
                     VLKRLCAWRERRAMAEDVPRNRVVWDEHLVEFARLGSLSETDVHARLPGPVARRYASE
                     LTAEHREGVSAGLAEPLLARPLSPSESKVSRTLRDLAWHKADTMEFARELLARKRDVE
                     ACIRHWLDTGELSPAYSGWRAGLIGEEFLAVLEHAP"
     gene            complement(2907..3506)
                     /gene="recR"
                     /locus_tag="AC16PP_13850"
     CDS             complement(2907..3506)
                     /gene="recR"
                     /locus_tag="AC16PP_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR6482758.1"
                     /translation="MGISPLIDRLIGAFQVLPGVGPKGAQRMVLHLLQRDREGARQLA
                     ETLLESLDRVSRCSRCRNLSEEAVCSICRDPRRDDDVLCVVESPADILAIEAAGGFRG
                     RYFVLHGRLSPIDGVGPEELGVDTLQRRVAELEPKEVILATNPTVEGEATAHYISKLL
                     KADVPNISRIAHGVPVGGELEYIDGGTIVHALQGRRSIS"
     gene            complement(3506..3823)
                     /locus_tag="AC16PP_13855"
     CDS             complement(3506..3823)
                     /locus_tag="AC16PP_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR6482759.1"
                     /translation="MKGLGDFMKQAQAMQERMQQVQEEIAKLEVVGESGAGLVKVTMN
                     GRHEVRRVHLDDSLLAEAKDVLEDLVAAACNDAVRKVEAAQRDKMAGMASGLGLPLDK
                     FPF"
     gene            complement(3820..>4771)
                     /locus_tag="AC16PP_13860"
     CDS             complement(3820..>4771)
                     /locus_tag="AC16PP_13860"
                     /inference="COORDINATES: protein motif:HMM:NF023592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HIR6482760.1"
                     /translation="EPEALETIAAAALGSMRDALSITDQAIAYGGGSLGHADVVEMLG
                     LVGRDEVHSLLEALASGDAERVLAMTAELAERGADFEEVLSGVIAAFHRMAVEQATGS
                     GANALFGPEVVQLYYQIALMGYRDMHIVPDPRSGFEMTLLRMIAFAPDEGLLTQSTAP
                     STTAERGAKERPVPESAGGRDGVESTLADGTPWHEVVESLEIGGVARMIAEHSVVGSH
                     QGDRLRLTLDTAHDTLLNSAQQAALQRALGGKLGSDLLLEIEAGSCAAETPAQRNARI
                     AAERQHQAEQTLKRDGVVQSLLSEFDGTLGDVKPMEDSAG"
BASE COUNT          878 a         1616 c         1434 g          843 t
ORIGIN      
        1 gtggaagaag cagcgacgca agaaggcatc gggcagctcc ttttcgttgt tcgacgtgat
       61 gacgacaacc ggccgctgct tcgccttgat ggtttcgccg agctcgtaga cgaaaaactc
      121 catccgatcg agttcctgaa gcaggtcgtt cggaaactcg atgtccgcct tgtcgatctc
      181 gtcgatcagc agcaccacgc gctcgtcggc ctcgaaagcc tcccagagct tgcccgtctt
      241 gatgtagttg cgaatgtcct gaacccgctc gtcgcccagc tgggaatcgc gcagccgcga
      301 caccgcgtcg tactcgtaga gcccgttgat cgccttggtg ctggacttga tgtgccactg
      361 aatcagtctg agcccaagcg acttggcgat ttcctcggcc agcatggttt tgccggttcc
      421 cggctcgccc ttgacgagca gcgggcgctc gagcttgacc gcggcgtcca cggccatgct
      481 cagctcatcg gtcgagatat aggtgtcggt actgtcgaag atcattcggc tctactcgtg
      541 aattgatgtt tcgcgcgagt gaaggacccc ccagaaaact gcgtcagcag gcgtcgcgcg
      601 tacgaagtcc gctactttaa tcgacgctcc gaggcctagc aaggcgcggc ccagcctgag
      661 ccccgtgcga ccgggagcat gatcctgtcc gcaagctcat tgccggacga ccaacgcaac
      721 cgggagttag atggcaaaga taaatatata taaaacaata ggttggatat cttttctaga
      781 gactactgtc agcgctttcc gcgttcggga gggacgtgct cgcccagcgc cgccagccga
      841 gcaccatcaa cacggcggcg cccgaggcca acgctgccac agccaggagg atcttgagcg
      901 cggtgaaagg atcgccgccc cgctgaaaga gcccttcgaa ggcgaggatg atcagagccg
      961 gcgcgtagct tccggcatac tctggaaccg gcgccggcgt gttgagcagg cacagaaaca
     1021 cgaaaacgac cgccagcctg atctcgcggg cgagcaaccc gctcagcgcg atatgcagcc
     1081 ccgctgcggc acccaggccg cccaccacga tagccgccca tacccatgcg ttccccatca
     1141 ggtctcaacc cactggatca gatgttcttc gaactcgtcc tcatgcaccg agcccgccgg
     1201 gatcactcga ccgagcaccg agatgcccgc tcgatgcacg gaatccgggg agccggaaac
     1261 gagagggtgc cagtcggcga gcccccgacc ttcggcgaca cggcggtagg cacagctgcg
     1321 cggcagccag gagagcgacg ccatcacctc cgcggagaag tcgatacagt ccttgacgag
     1381 gcggtgtctg tccggataac gggtgcagcg acaggtctcg aggtccagca gatcgcaaac
     1441 cagccccgtg tagtggacgc atcccgaacc ctcctcttcg agcttgacga ggcaacatcg
     1501 ggcgcagcca tcgcagagcg actcccactc ctcggccgac agctcaccaa gcgtcttgtg
     1561 ctcccagaac cggtccatca ggaaagctcg tcgaccgatg gcggcatctg gaggtagaag
     1621 ccgcactctt cgatattctt gagcacggcc gctgcatccg ccctggccag ctgccggtcc
     1681 ctggtcagcg cgagggacat ggccgccacc ggtcgcccga agcttttcat gagcgcttcc
     1741 ggtacacggg cgaggtcctc gccggcgtcc acgtacagat acatgccatt ccggcgcgag
     1801 ctgcgataga tctgcaccag gcggctcaag gcgcgtgctc cagaaccgcc aggaactcct
     1861 caccgatcaa gccggcccgc cagccggagt aggcgggcga cagctcgccg gtgtccagcc
     1921 agtggcgaat gcaggcttcc acgtcacgct tgcgggcgag cagttccctg gcaaactcca
     1981 tcgtatcggc cttgtgccag gcaaggtcgc gcagggtccg gctgaccttg ctctccgacg
     2041 ggctcaaggg tcgagcgagc agcggctcgg cgagcccggc cgagacgcct tcccgatgct
     2101 cggctgtgag ctcggaggcg taccggcggg caacagggcc cggcagacgc gcgtgcacgt
     2161 ccgtctcaga aagcgatccg aggcgcgcga attccaccag atgctcgtcc cagaccaccc
     2221 ggttgcgagg cacgtcttcg gccattgccc ggcgctcgcg ccacgcgcaa agccgcttga
     2281 gcacaccgag ctcaagagcg tccaggcgcc aggccttctt gacgccacgg tagtattcat
     2341 ccggatgggc tccctgaacc cgacctttct cggccatggc gtcagcgaac cacgagcgtc
     2401 tgccgagttc gtcgagccgc gcggtcagcg cttcgtaaac ttcgggaagg tagcgcacat
     2461 cctcccaggc atagtgcagc tgctcctcgg tcaggggccg gcgcaaccag ttggaccggg
     2521 tggcgtgctt gcccagatcg tgtcccagca atgccgctac caggttggcg tagctcacgc
     2581 tgtacctcga gttctgaaac gcataggcca gctgggtatc gaatatcccc tggggttcca
     2641 ccccgaggtg atgacgaatg agctcgaggt cctcggagca ggcgtgcatc accttgacga
     2701 tggccggatc gtgcagcacg tcggcaaacg ccgagaaatc gtcgatggac agcgggtcga
     2761 ggaaatacac gtcctcgccg ctgatgacct gataaaggcc gggcaccgga aaaaaggtgt
     2821 ccgtgcgctg aaactccgtg tccagaccga tcacgtcgcc ccaggacgcc accgcgtcga
     2881 ccagagcgcc agtgtcgtcg atccagctaa ctgatgctgc gtcggccctg caaggcatga
     2941 acgatcgttc ctccgtcaat gtattcgagc tccccgccaa cgggcacccc gtgggcgatt
     3001 cgcgatatgt tgggtacgtc ggccttcagc agcttgctga tgtaatgcgc cgtggcttcg
     3061 ccctccaccg tggggttcgt ggcgagtatg acttccttcg gttccagctc cgcgaccctg
     3121 cgctgcaacg tgtctacgcc gagttcctcg ggccccaccc catcgatggg ggacagccgt
     3181 ccgtgcagga cgaagtagcg tccgcgaaag ccgcccgccg cctcgatggc gagaatgtcc
     3241 gcgggcgact cgaccacgca gagaacgtca tcgtcgcggc gcggatctct acagatcgag
     3301 cagacggcct cttccgacag gttccggcag cgtgaacagc gcgacacgcg gtccagggac
     3361 tcaagaagcg tctccgcaag ttgccgggcg ccctcacgat cgcgctgcaa caggtgcagg
     3421 accatgcgtt gcgctccctt ggggccgact ccgggaagca cctgaaaggc cccgatcagc
     3481 cggtcgatta gcggactgat tcccatcaga aagggaactt gtcgagcggc aggccgagac
     3541 cggatgccat tcccgccatt ttgtcccgtt gggccgcttc caccttccga accgcgtcgt
     3601 tgcaggcggc agcgaccagg tcctcgagca cgtctttcgc ttccgcaagc aggctgtcgt
     3661 cgagatggac gcgccgaact tcgtgccgcc cgttcatcgt gaccttgacg agaccggccc
     3721 cgctctcgcc cacgacttcg agcttcgcga tttcctcctg aacctgctgc atgcgttcct
     3781 gcatggcctg ggcctgtttc atgaagtcac cgagcccttt catcctgccg aatcctccat
     3841 gggtttgacg tctcccaggg tgccgtcgaa ctcgctgagc aacgactgca cgaccccgtc
     3901 tcgcttgagg gtctgctcgg cctgatgctg acgctccgcc gcaatgcgcg cgtttcgctg
     3961 cgccggtgtc tccgccgcgc agcttcccgc ttcgatctcg agcagcagat ctgatcccaa
     4021 cttgcctccg agggcgcgct gaagcgccgc ctgctgggcg gagttgagca acgtgtcatg
     4081 ggccgtatcg agcgtaagcc gcagccggtc cccttggtgg gaacccacca cgctgtgctc
     4141 tgcaatcatc cgtgcgacgc cgccaatttc aagggactcg accacctcgt gccacggcgt
     4201 gccgtcggcc agggtcgact cgactccgtc gcgccccccg gcgctttccg gcaccggccg
     4261 ctcctttgct ccccgttcgg ccgtcgtcga gggcgcagtg ctttgagtca gaaggccctc
     4321 gtcgggagca aacgcaatca tacgcagcag ggtcatctcg aaaccgctac gcggatccgg
     4381 aacgatgtgc atgtcccgat agcccatgag cgcaatctgg tagtagagct gaaccacctc
     4441 cggcccaaag agcgcgttgg ctcctgaacc cgtcgcctgc tccacggcca tacggtgaaa
     4501 ggccgcgatc acgccgctta gaacttcctc aaaatccgcc cctcgctctg ccagctccgc
     4561 cgtcatggca agcacccgct cggcgtcgcc gctggccagt gcttccaaca ggctgtgcac
     4621 ctcgtcgcgt cccaccaggc caagcatttc taccacgtcg gcgtggccga gagacccgcc
     4681 gccgtaagcg atggcctgat cggtgatgga aagcgcgtcc cgcatgctgc ccagcgcggc
     4741 cgcggcgatg gtctcaagtg cctcgggctc g
//
LOCUS       DBNHKV010000425        10763 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_413093, whole genome shotgun sequence.
ACCESSION   DBNHKV010000425 DBNHKV010000000
VERSION     DBNHKV010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10763)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10763)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10763
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_413093"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1012
                     /locus_tag="AC16PP_13865"
     CDS             197..1012
                     /locus_tag="AC16PP_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6482761.1"
                     /translation="MSEGPATEQFGEFTTLERRGRVVVASFDRGDGLNALSSNLMREL
                     TALAGELADDTESSVVVLTGRGAFSAGADLKEPDAGASRTPTLLERRQALSIGPDLCA
                     AWERLEQITIAAVEGFCIGGGVALAVACDHRVAGSSAHFRLPEVPLGMNMSWQSNPRT
                     VALIGPSRAKLFTILGEKLAADTALEWGLVDEVVADGQALASAVSLADRYAAMPPLPL
                     RMTKQAINAATGALNYATSYMDRDQFLLTALSDDQKEGVRAFLEKRTPEFRGD"
     gene            complement(1142..2770)
                     /locus_tag="AC16PP_13870"
     CDS             complement(1142..2770)
                     /locus_tag="AC16PP_13870"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6482762.1"
                     /translation="MKDHDVKPLGRLLTAAAAAHPDRDAMVLVDERATYAELVCRAKR
                     AGRSLMALGVEPGDRVGILMANCLDFVDILFGTTLIGAVPVLYNARFKAREIAHVTAD
                     AGVKVVVTNDIVEQHTNYVELLRRSLPGLAEHDAIRTLALKAAPDLRTAVTLSSSSTA
                     GFLNRNAFYELGSTHPEEVVDERGARVRPEDVAVMIYTSGTTAMPKGCPLTHIVLQHA
                     GVVGGVERLGLIEGDVMWAPLPMFHTAFTQPLTGILHVGGTFLSMTHFDAAAALRMIH
                     DEQATVMFPAFPTITMQLLDHPSYTAQSFACVRVAINVGPPEELCAMQERMPHTTQIT
                     VFGMTETGGSAAMCERDDSLAHRTETSGFALPGNEIQIRDPDSGAELPAGERGEIVVR
                     GRGVFSGYHKAPEKTAESFYDGGWFRTGDLGVMDADGRVTFRGRLKDMLKVGGENVAA
                     VEVEGFLATHPAVNLAQVVAVPDDKYGEVPAAFVELAPGASASEQEIIDYCKDEIASF
                     KIPRFVRFVEEWPMGATKILKYELRERICSELGL"
     gene            3022..4557
                     /locus_tag="AC16PP_13875"
     CDS             3022..4557
                     /locus_tag="AC16PP_13875"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6482763.1"
                     /translation="MVETVTGTNRSAGVSYSEVLDRDSHPVRDILRVDNPLPPGPTIV
                     DPKVYYSREVFEREKERLWKRVWQMACHEDDIPNVGDAIVYDIADLSYMVVRSAEDEF
                     HAFPNACLHRGRQILHQNEERLHNLRCAFHGWSWGLDGELQEVPCHWDFPTVSKETHS
                     LKQVKVGRWGGFVFINPDPDCEPLEDFLGNLDEHMTLLPMERRYKAVHVAKRLPCNWK
                     VAHEAFAEAYHSVATHPTLLSTLGCANSKYDIFGNFSRALSAHHVGSPHVPSWLAEHP
                     YLEATPFTKQRHELTGSIYERVDDGRVKVTTTDNRVGYFDDRGNHIDGEKFHADIHLC
                     NWVGGKLIEGMDELPSSVITGDFAEKRAKAAAVRREELRKILGDKMDEVSDAELLDPI
                     YYSLFPNVSPWGSFNQIFYRFRPYGDNPEECIHECLFMWPVAEGQPRPPAAKMQWLDL
                     DDDYVDAPELGHLAKVFNQDTLNLPYVQKGLHSLDEVIFANYGETKPRHFHMLYNEWM
                     SKE"
     gene            4670..6631
                     /locus_tag="AC16PP_13880"
     CDS             4670..6631
                     /locus_tag="AC16PP_13880"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR6482764.1"
                     /translation="MMKLLSPVEVGPIEVKNRVVSTAHAAFLEFFNPVSDGERYMAYQ
                     ERRAQGGTGLIIFTAMHVHESSQIPYHYVFDADTMAPKFAQISTRLHRHGAKCISQLF
                     HFGAQGKSDPRDDFHPLWSFSGTTTLEGEVTHKMTDAEIEEVIDAFAFAAKVATENGM
                     DGVELHGTHGYLIQQSFSPFANQRDDKWGEELVFVKTLARRVRDAIGPGKIMGFRISA
                     DDFIDPADGGVGHERLCHIASEVVGTGLFDYLNHSEGAGGVHYARAIGSFRHKFGEYL
                     PLTRNLRDAIGGAVPLIGVGKIPTPDLAEQALQAGDCDLVGMTRAQIADPDLVSKVAA
                     GRAHRVRTCTGSNQGCFERASYPITCFQNPEVGEENRFKALDIPVVQLKKVLVVGGGP
                     AGMKAAEIAARRGHSVTLAESGDRLGGRLNLVETAGDATNLLSATAWIEEELGGLDVD
                     IRLQTTVDEAFVRQVNPDAIVLATGAYVTNDLGVATDGSVQVLSTDDAASGTFEGNRF
                     ELEGTRCLMIDFRGNYETAVVLESLGRRGSKVTLVTPAPVFGRNIGVSHADDFRRTVI
                     PQLGIRLLPNTTLAQISQGRGHLVNHVTGEEIEEFFDFIVAGTPPRPRDVLFEVLGRH
                     APVTVVGDAVAPRDAMLAFREGDRAGRTI"
     gene            7048..7569
                     /locus_tag="AC16PP_13885"
     CDS             7048..7569
                     /locus_tag="AC16PP_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482765.1"
                     /translation="MKKTSAIALTTLGLLAASSAMAATDGSYDTTSTGTTDVSLTVVD
                     ATRITGLDDIDLGTYAATDTDGVDASDTFCVHVNGGDAYNITATYGPLASPTTSDTIA
                     VQVSLIDNAAGTGAEDVNTGGVSSNYTGAALIDCGGSDNAYINVEVEDADIRAATTAS
                     DYATTVSLLVSPQ"
     gene            7755..8699
                     /locus_tag="AC16PP_13890"
     CDS             7755..8699
                     /locus_tag="AC16PP_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482766.1"
                     /translation="MTYAANIGNDNYLSVGGVPDVDFGTWSGGSLSTTVRGCIASANE
                     RQPNPPSPPAEVYPYQAGVDNRNPGSDFYLYLNNDASSPESARITVVVEHRDIVAGSA
                     FETLTEGVFDTHYHLGRFQECLGTGDNSELRISIPEANLTNKVAGVYHGQFRLEAVGG
                     TSGTKYDRTDFDLILTVQSANVRISGMDDMALGTYVPGTGNVRQDEHFCVQSLADNAA
                     YRISVSSSNQTPGGDFRLKHATDAVYLPVDVLFSSVGSGSGTEQITNNSISALGSPDS
                     FCGGSDNATITVRALEADVQAVPSGSYGDTLVLLVVPE"
     gene            8705..9280
                     /locus_tag="AC16PP_13895"
     CDS             8705..9280
                     /locus_tag="AC16PP_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482767.1"
                     /translation="MLARLRTASVNALCLGIALLVVVGGGSARGAQPNGIDGAFGETS
                     KAEARVTLRIRSGARIVRGPGTGGRPDAPPGGGRGRPDLNLSSRDLSETGEAVLPLCL
                     NAPSGQAFEVSALPDGGDEASGLRTASGSAVSYEVSLGAEGGASATFISSGRDCTPEN
                     SLRLSISTTRSAPDTELTNLTGHVRLFVVPV"
     gene            9318..10049
                     /locus_tag="AC16PP_13900"
     CDS             9318..10049
                     /locus_tag="AC16PP_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043711 - pilus organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis chaperone"
                     /protein_id="HIR6482768.1"
                     /translation="MKTERFHICSIIVCSMVALATGSACAELVVNRAIVVFDSPSQHR
                     QDVELVNIDAGDNLFVDIESFQVERPGEDDQALTSLRGNENPGLIATPQKAMIGPSNS
                     QLVRFMNLDRDGDREKVFRVNMTPVTRPPEFDAGDVARGETASQIQIVIGYQVLVIVP
                     PVDPRAVVDYGRSGSSATFKNSGNSNYLLTDGRQCDPDAPEVCMDLVSYRVYPGNHRQ
                     VELPFDGPFSYKIRTVAGITSQRFE"
     gene            10129..>10763
                     /locus_tag="AC16PP_13905"
     CDS             10129..>10763
                     /locus_tag="AC16PP_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482769.1"
                     /translation="MLATGAVRATEGGPANVDEQPVVAAVPTRLHPAPIPPARRDTWI
                     PPGFEDLAAPQTTLVDVFYGGDFLISTMVRFSLDEVVILHPEAVADRLPDLIDPRGFT
                     EKLARAFYPNSELLCRSKYQQDCELIETDDVDLIFDRHRLQLSLFIGSPLLRTRTTEQ
                     MKYLPASDAGASIMNDTAAYYSGFVGEDLTYNVASESVFAFAESRISARSNL"
BASE COUNT         1950 a         3452 c         3401 g         1960 t
ORIGIN      
        1 ccgattcctt cggtgcgagc ctggaccatg tccctatcct cgccccgtca gcccaacctg
       61 atcctcgttg tgcccgtcat ccttgcgctc gtcgggatgt caatgaccca aagcgaagac
      121 ggctgaaacc gcggagccgg ggctcggctc gggcggcttt tgttaagatc gggcaggacc
      181 agccagggag gcgaacatga gcgaagggcc cgccaccgag caattcggcg agttcaccac
      241 gctggaacgt cggggccgcg tcgtcgtcgc ttcgttcgac cgcggcgatg gcttgaatgc
      301 cctttcctcc aacctgatgc gcgaactcac ggcccttgcc ggtgagcttg ccgacgacac
      361 ggaaagctcc gttgtcgtcc tgaccggtcg gggcgcgttt tcggcgggtg ccgatctgaa
      421 ggaacccgac gcgggcgctt cccggacgcc gacgttgctc gagcggcgtc aggctctttc
      481 gatcgggccg gacctctgtg ccgcctggga gcggctcgag cagatcacca ttgctgcggt
      541 ggaaggcttc tgcatcgggg gtggcgttgc cctcgctgtt gcgtgcgacc atcgggtggc
      601 cggctcctct gcccacttcc ggctgccgga agttccgctg gggatgaaca tgagctggca
      661 gagcaatccc cgcacggtgg ctctgatcgg tccctcccgc gccaagcttt tcaccatcct
      721 cggagagaag ctcgccgcag acaccgcgct ggaatggggc ctcgtggacg aagtggtcgc
      781 cgacgggcag gcgctggcgt cagccgtttc gctggccgat cggtacgcgg ccatgccccc
      841 gctgcccctg cgaatgacca agcaggccat caacgccgcc accggggccc tcaactacgc
      901 aacgagctac atggatcggg accagtttct gctcaccgcg ctgagcgatg accagaagga
      961 gggcgtgcga gcctttctgg agaaacgaac gccggaattt agaggagact gatccaggcg
     1021 cgggcagggc gggttcaaag ccctgtccag gcaaccgggc gctgcctggc cgcgcctcag
     1081 cgttgtcttt ggggggaagc tccgtacaga ccgccctcga cggggacccg ctcgacgctc
     1141 gtcagagccc gagctccgag cagatgcgct cgcgcagctc gtatttcagg atcttggtcg
     1201 cgcccatggg ccactcctcc acgaagcgga cgaatcgggg aatcttgaag cttgcgatct
     1261 cgtctttgca gtagtcgatg atctcctgct cgctcgctga ggctccgggc gcgagctcga
     1321 cgaaggcggc tggtacctca ccgtacttgt cgtcgggaac ggcgactacc tgtgccagat
     1381 tgacggcggg atgggtggcc aggaacccct ccacctccac cgcggcgacg ttttcgccgc
     1441 cgaccttgag catgtccttg agccggcccc gaaacgtcac ccggccgtcg gcgtccatga
     1501 ctccgaggtc gccggttctg aaccagccgc cgtcgtagaa tgattctgcg gtcttctccg
     1561 gcgctttgtg gtagccggag aacacgccgc gtcccctgac gacgatctct ccccgctcgc
     1621 cggcaggcaa ctcggcgccg ctgtccggat cgcggatctg aatctcgtta cccggcagcg
     1681 cgaacccgga ggtttccgtg cgatgggcca ggctgtcgtc gcgctcgcac atcgccgccg
     1741 acccgcccgt ctcggtcatg ccgaatacgg tgatctgcgt ggtgtgcggc atgcgttcct
     1801 gcatggcgca aagctcctcg ggaggcccta cgttgatcgc gacgcgtacg caggcaaagc
     1861 tttgcgcggt gtacgaaggg tggtcgagca gctgcatggt gatggtcgga aacgccggga
     1921 acatgaccgt cgcctgctca tcgtgaatca tgcgcaaagc cgccgcggcg tcgaagtgcg
     1981 tcatggagag gaaggtgccg cccacgtgca gaatgcccgt cagtggttgg gtaaacgccg
     2041 tgtgaaacat cggcagcggt gcccacatca catccccttc gatgagcccg agccgctcca
     2101 cgccgccgac cacgccagcg tgctgcagca cgatgtgggt gagagggcag cctttcggca
     2161 tggcggtggt tcccgaggtg tagatcatca cggcgacgtc ctccgggcgg acccgggcgc
     2221 cgcgctcgtc gacgacctcc tccggatgcg tcgagcccag ctcgtagaac gcgtttcggt
     2281 tcaggaagcc ggccgtgctc gatgacgaga gggtcaccgc cgtgcgcaga tcgggcgccg
     2341 ctttcagggc cagcgtgcgg atggcgtcat gctcggcgag acccggcagg gagcgccgga
     2401 gcagctccac gtagttggtg tgctgctcga cgatgtcgtt ggtgaccacc accttgacgc
     2461 cggcgtcggc ggtgacgtgc gcaatctccc gggccttgaa gcgggcgttg tagagcacgg
     2521 gcacggcgcc gatgagcgtc gtgccaaaca ggatgtccac gaagtccaga cagttggcca
     2581 tcaggatgcc cacccggtcg ccgggttcca cgcccagcgc catgagcgag cggccggcgc
     2641 gctttgcccg gcagacgagc tctgcgtagg tggcgcgttc gtccacgagc accatggcat
     2701 cgcggtcggg gtgtgcggcc gcggcggcgg tcaatagccg gcccagaggt ttgacgtcgt
     2761 ggtctttcat gggcaaggcg aattctcgtc ggcgtggtga gaaagcacag gcacttcgga
     2821 tatttccgct aagcataacc ggacgatagt gtaagggctt gagcggcgac gcgcatgcag
     2881 ccgcccgtga aatgacgccc ttttgctagc gttaccagct cctcccggag ttggccgtac
     2941 gcagtttccg ctattgcccc ccggtctgat agatgcgctg atagatgagg cgcatttgat
     3001 ctgctcggag ggggcgaatt catggtcgaa acagtaacgg gcacgaaccg atccgcaggc
     3061 gtcagttaca gcgaggtgct cgaccgcgac tctcacccgg tgcgggacat cctgcgtgta
     3121 gacaacccgt tgccgccggg cccgacgatc gtcgacccga aagtctatta ctcccgtgag
     3181 gttttcgaac gggagaaaga gcgcttgtgg aagcgggtct ggcagatggc gtgccacgag
     3241 gatgatattc ccaacgtcgg cgacgccatc gtctacgaca ttgcggacct ttcctacatg
     3301 gtcgtgcgca gcgcagagga cgagttccat gcgttcccga atgcctgcct gcaccgcggc
     3361 cggcagatcc tgcaccagaa cgaagagcgt ctgcacaatc ttcgctgcgc tttccacggc
     3421 tggtcctggg ggctcgacgg cgagctgcag gaggtgccct gtcactggga tttccccacc
     3481 gtctcgaagg agacccactc cctgaagcag gtgaaggtcg gccgctgggg cgggttcgtg
     3541 ttcatcaatc ctgatccgga ttgcgaaccg ctggaggact tcctcggcaa tctggacgag
     3601 cacatgacgc tgctgcccat ggaaagacgg tacaaagccg tgcacgtcgc caagcggctg
     3661 ccgtgcaact ggaaggtcgc ccacgaggcg ttcgccgaag cctatcactc ggtagcgacc
     3721 cacccgacgc tgctcagcac gctggggtgc gccaactcaa agtacgacat cttcgggaac
     3781 ttttcccgcg ccctgtcggc ccatcatgtt ggcagccccc acgtgccatc gtggctggcg
     3841 gagcacccct atctggaggc gacgccgttc acgaagcagc gtcacgagct caccggcagc
     3901 atttacgaac gagtcgatga cgggcgcgtc aaggtcacca ccaccgacaa ccgcgtgggc
     3961 tacttcgatg atcgtggcaa ccacatcgat ggtgagaagt tccacgccga cattcacttg
     4021 tgcaactggg tgggcgggaa gctgatcgag gggatggacg agctgccctc ttcggtcatc
     4081 acgggcgact ttgccgaaaa gcgtgcgaag gcggctgccg tgcggcgcga agagctgcgc
     4141 aagattctcg gcgacaagat ggacgaggtg agcgacgcgg agctgctcga tcccatctac
     4201 tacagcctgt tccccaacgt cagcccctgg gggtctttca accagatctt ctaccgtttc
     4261 cgaccttacg gcgacaaccc ggaggagtgc atccacgagt gtctgttcat gtggccggta
     4321 gccgaaggac aaccgcgtcc gccggcggcg aagatgcagt ggctggatct ggacgatgac
     4381 tatgtcgacg cgccggagct cggacatctc gcgaaagtct tcaaccagga caccctgaac
     4441 ctgccctacg tgcagaaggg gctgcattcg ctggacgagg tgatcttcgc caattacgga
     4501 gaaaccaaac cgcgccactt ccacatgctc tacaacgagt ggatgtcgaa ggagtaagca
     4561 aggcgggcgg aattcgctga gccgagcggg ggccgatgcc tgtcgaacga atcgagacat
     4621 ttgctctgtg gcggtactac actcgaagta tcggtctgcg aggaacgcca tgatgaagct
     4681 gctcagccct gtcgaggttg gcccgatcga ggtgaagaac cgggtggtct ccaccgccca
     4741 cgctgcgttc ctcgagttct tcaatcccgt ctccgatggc gaacgctaca tggcgtatca
     4801 ggagcgtcgt gcccagggcg ggaccgggct gatcatcttc acggccatgc acgtacacga
     4861 atcgagccag atcccctacc actacgtttt cgatgcggac accatggcgc cgaagttcgc
     4921 gcagatctcg acgcggctgc atcggcacgg ggcgaaatgc atctcccagc tgtttcattt
     4981 cggtgctcag ggcaagtcgg accctcgcga tgactttcat cctttgtgga gcttctccgg
     5041 aaccacgacg ctggaaggcg aggtcaccca caagatgacc gacgcggaga tcgaggaggt
     5101 catcgatgct ttcgccttcg ccgccaaggt cgctaccgag aacggcatgg acggcgtgga
     5161 gctgcacggc acccacggct atctgatcca gcagtctttc agcccctttg ccaaccagcg
     5221 cgacgacaag tggggtgagg agctcgtttt cgtgaagacc ctggcgcgcc gggttcggga
     5281 tgccatcggg cctggaaaga tcatgggatt tcgaatctcg gccgacgact tcatcgatcc
     5341 ggccgacggc ggcgtgggcc atgagcggct atgtcacatc gcctccgagg tggtgggcac
     5401 gggcctcttc gactacctga accattccga gggtgccggc ggcgtgcact atgcgcgcgc
     5461 catcggctcg ttccggcaca agttcggcga gtacctgccg ctgacgcgca acctgcgcga
     5521 tgcgattggg ggtgcggtgc ccctcatcgg tgtggggaag attccgaccc cggatctggc
     5581 ggagcaggcg ctccaggccg gcgactgcga tctcgtcggc atgacccgcg cgcaaattgc
     5641 ggatcccgat ctggtcagca aggtggccgc gggccgagcc catcgggtgc gcacctgcac
     5701 cggctccaac cagggctgct tcgagcgcgc ctcgtatcca atcacctgtt tccagaaccc
     5761 cgaggttggt gaggaaaacc gcttcaaagc cctcgacatc cccgtggtcc agctcaagaa
     5821 ggtgctggtg gtgggcggtg gtccggcggg catgaaggcg gctgagatag ccgccaggcg
     5881 cggacactcg gtcaccctgg ccgagtctgg tgaccggctt ggcggacggc tcaacctggt
     5941 ggaaacggcc ggcgacgcca ccaacctgtt gagcgcaacc gcatggatcg aagaggagct
     6001 tggcggcctg gatgtcgata tcaggctgca gaccaccgta gacgaagcat tcgtccgcca
     6061 ggtgaatccg gacgccatcg tcctcgccac cggcgcctac gtgaccaatg acctcggcgt
     6121 cgcaaccgac ggttccgtgc aggtgctgtc caccgatgac gcagcctccg gcaccttcga
     6181 gggcaaccgc ttcgagctgg aagggacgcg ctgtttgatg atcgatttcc gcggcaatta
     6241 cgaaacggcc gtggtgctcg aatcgctcgg acgccggggc agcaaggtta ccctggtgac
     6301 cccggcgccg gtcttcggcc gcaacatcgg cgtctcccac gccgacgact tcagacgcac
     6361 ggtcattccg cagctgggaa tccgcttgct gcccaacacg acgctggcgc agatcagcca
     6421 gggcaggggc catctggtca atcacgtgac gggtgaggag atcgaagagt tcttcgactt
     6481 catcgttgcc ggcacgccgc cccggccccg ggacgtcctc ttcgaggttc tcggtcgcca
     6541 tgccccggtg acagtggtgg gcgatgccgt cgcgccgcgg gatgcgatgc tggcgttccg
     6601 cgagggggac cgggcgggcc gcaccatctg acccccgcgt gcccgatctc tctgccgcca
     6661 tgacgcgctg ggcacaaatc gtgttcctgg cttctcggct cgactgacga aggcgtcaca
     6721 gcgtgggtcg aaatgtgacc cgctgtgaca atcgcgcgcg atccatgtcg gctacggcga
     6781 gcagcggccg ctcgtcggga aaatttcttc gctcgtcgct aacaagctga aattcataag
     6841 aatttcaggc ggcgacgcgg ccagcaaaat tgcattgccg gaaagttggc acgccccctg
     6901 caccaggggc tcgaagcttc gatgcagtat gccgggatcc cagctcaggc agatcggaac
     6961 tcagcaacgg ctgttgaagc gcgcggcaga tccttcacct gaaggtcgtc gtaaacgtgt
     7021 gactcaacta ccaatggaat ctgaacagtg aaaaagacct ccgcaattgc cctgactacc
     7081 ctcggcctcc tcgccgcaag ctccgccatg gcggccaccg acgggtccta cgacacgacc
     7141 agcaccggca ccaccgacgt cagcctgacg gtcgtggatg ccacccgcat caccgggctg
     7201 gacgacatcg acctcggcac ctacgcagcc accgacacgg acggcgtgga cgcttcggat
     7261 acgttctgcg tgcacgtcaa cggcggtgac gcatacaaca tcacggcaac ctacggaccc
     7321 ctggcgagcc cgaccacgtc ggacacgatc gccgtgcagg tgtcgctcat tgacaacgct
     7381 gccggcacgg gcgccgaaga cgtcaacacc ggaggtgtct cttccaacta cacgggagct
     7441 gccctgatcg actgcggcgg cagtgacaac gcgtacatca acgtcgaggt ggaggatgcg
     7501 gatatccgcg ccgcgacgac ggccagcgat tacgccacga ccgtctcgct gctggtttcg
     7561 cctcagtgac ccggggcggt gacctggtgc ccgaacacgg gcaccaggcc cgcggcgccc
     7621 ggtttccgag ctgaccgaca tgttttcact caccgagctg atccgacttg caccccattc
     7681 ctctacacag atggccgcaa cttccgctcc tgctcaccgg gctttgcgtc ctgctgtccg
     7741 ccgggtttcc taagatgacc tatgccgcaa acattggtaa cgacaactac ttaagcgttg
     7801 gcggcgtccc cgacgtggac ttcggcacct ggtccggcgg aagcctgtcc accaccgtgc
     7861 gcggctgcat tgcttctgcc aatgagcgcc agcccaaccc gccgagcccg ccggcggagg
     7921 tttacccgta ccaggccggt gtggacaacc gcaatccagg ctcggacttc tatctttatc
     7981 tgaacaacga cgcctcgagt cctgaaagtg cccgcatcac cgttgtggtc gaacaccgcg
     8041 acatcgttgc ggggagcgcg ttcgagaccc tcactgaggg ggtctttgac acgcactacc
     8101 atctcggccg cttccaggaa tgccttggga ccggggacaa ctcggaactg cgcatctcca
     8161 ttccggaggc gaacctgacg aacaaggtgg ccggggtcta tcacgggcag ttccggctcg
     8221 aagcggtggg gggaacgagc gggacgaagt acgaccggac cgatttcgat ctgatcctca
     8281 ccgtgcagag cgcaaacgtc cgtatcagcg gtatggatga catggcgctg ggtacttacg
     8341 tccccgggac cggtaacgtt cggcaggacg agcatttctg cgtgcagagc ctcgcggaca
     8401 acgctgccta ccgcatcagc gtctcatcca gcaatcagac ccctgggggc gattttcgac
     8461 tgaagcacgc cacggatgcc gtttacctgc ctgtggacgt gctcttctct tctgtagggt
     8521 ctggcagcgg cacggagcag atcaccaaca actccatctc ggccctgggc agccccgaca
     8581 gcttctgcgg cggctccgac aacgccacca tcaccgtgcg cgccttagag gccgacgtgc
     8641 aggccgtccc ctcaggctcc tatggggaca cgctggttct gctggtcgta ccggagtagc
     8701 ggcgatgttg gcacgtttgc gaactgcttc ggtcaatgcg ctctgccttg gcatcgcctt
     8761 gctggtcgtc gtcgggggcg gaagcgcccg gggcgcccag ccgaacggga tcgatggggc
     8821 gtttggcgag accagcaaag cagaagcgcg cgtgacgttg cgaatccgga gtggtgcgcg
     8881 gattgtcagg ggacccggta ccggcgggcg cccggacgcg cctcccggcg gcgggcgagg
     8941 gagacccgac ctgaatctgt cgagccggga tctcagcgag acgggtgagg cggtgctgcc
     9001 gctgtgtctc aacgcgccca gcgggcaggc ctttgaagtg tccgcgttgc cagacggcgg
     9061 tgacgaagcc tccgggctgc gcacggcatc gggctccgcc gtgagttacg aggttagcct
     9121 tggggccgaa ggaggcgcgt cggcgacctt catatccagc ggtcgcgact gcacgccgga
     9181 gaactctctt cgcctgtcga tcagcaccac ccgatctgcg ccggacaccg aactcacaaa
     9241 cctgaccggc cacgtccggc tgttcgtcgt tcccgtgtga ccacgcacat caaccagacg
     9301 ccaaatagga ggctgccgtg aaaactgagc gtttccatat ctgttcgatc atcgtttgct
     9361 ccatggtcgc attggccacc ggctccgcgt gcgcggagct ggtggtcaat cgcgccattg
     9421 ttgtcttcga ctctcccagc cagcatcgcc aggatgtgga actggtcaac atcgacgccg
     9481 gtgataacct tttcgtcgac atcgaatcct ttcaggtcga gcggccggga gaggacgacc
     9541 aggccctgac ctcgttgcgg ggcaatgaaa atcccgggct gatcgcgacg ccgcagaagg
     9601 ccatgatcgg cccctccaac agccagctcg tgcgcttcat gaacctggat cgcgacggcg
     9661 atcgggaaaa agtatttcgg gtgaacatga cgcccgtgac caggccgccg gaattcgatg
     9721 cgggagatgt tgcacgaggc gaaaccgcct cgcagatcca gatcgtgatt ggttatcagg
     9781 tcttggtgat cgttccgccg gtagatcccc gggcagtggt tgactacggc cgctcgggaa
     9841 gctccgcgac cttcaagaat tccggaaact ccaactacct tctcaccgac ggtcgtcagt
     9901 gtgatcctga cgcgccggag gtttgcatgg accttgtttc ctatcgggtt tatccgggca
     9961 accacaggca ggtcgagctc cccttcgacg ggcccttcag ctacaagatt cggacggtgg
    10021 cgggcatcac cagccagcgc ttcgagtagt tgcacggcct tcgctcgcta gatgtttcgc
    10081 aggccgccga cgtggcaagt agcctggacc ctggtcgcgg caaccctgat gctcgcgacc
    10141 ggcgccgtcc gggcgacgga gggcggccct gcaaacgtcg acgagcagcc ggtggtcgcc
    10201 gccgtgccca cccggctgca tccggcgccg attcccccgg ctcgtcggga tacctggatt
    10261 cccccgggtt tcgaagacct tgctgcgccg cagaccactt tggtggacgt cttctacggc
    10321 ggagatttcc tcatttccac catggtccgc ttcagcctcg acgaggtcgt tatcctgcat
    10381 ccggaggcgg ttgccgaccg cctgcctgac ctgatcgacc cacggggatt tacggaaaag
    10441 ctcgctcggg ccttctatcc gaattccgag ctgctgtgca gatccaagta tcagcaggac
    10501 tgcgaactca tcgaaaccga cgacgtcgac ctgattttcg atcgccaccg ccttcagttg
    10561 agcctcttca tcggttcgcc gctgcttcgg acgcgcacaa cggagcagat gaaatacctg
    10621 cccgcgtccg atgcaggggc ctctatcatg aatgacacgg ccgcctacta cagcgggttc
    10681 gttggcgaag acctgaccta caacgtcgcc agtgagagcg tcttcgcttt tgccgagagt
    10741 cggatctcgg ctcgcagcaa cct
//
LOCUS       DBNHKV010000426         3380 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1762822, whole genome shotgun sequence.
ACCESSION   DBNHKV010000426 DBNHKV010000000
VERSION     DBNHKV010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1762822"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="AC16PP_13910"
     CDS             <1..641
                     /locus_tag="AC16PP_13910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6482770.1"
                     /translation="VGRSGCGKSTLLKLLNGLVVPDAGRVSAFGTQLDYADLVSVRRR
                     MGYAVQGTGLCPHMSARENVVLSAEIAGWKEQAVAQRLGELLELTRLSAELLAKYPHE
                     LSGGQQQRVGLCRAMMLRPEVLLLDEPFAAIDPITRYDIHHQLLEILAAEPATVVLVT
                     HDMREAMQLADRIVLLEGGRVFLDESRDSLARSRPGVEPEALLQSLMEEALA"
     gene            638..2113
                     /locus_tag="AC16PP_13915"
     CDS             638..2113
                     /locus_tag="AC16PP_13915"
                     /inference="COORDINATES: protein motif:HMM:NF015995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR6482771.1"
                     /translation="MKGACLLLAAACIVLVAPVRAAERVTVASKAFSESYVLAEIAAQ
                     LLEAEGFEVERKLGLGGTLIAFGALREGAVDVYPEYTGTLTQAILDEPGMTIAELDAA
                     LAERGLVFRVPLGFNNSYAVAVTEEMAGARGLTRVSDLAGQTDLSFGFSHEFLNRADG
                     WPALKTAYGLTQTASGIEHALAYRALASGHLDVTDAYTTDGELSLYALRLLADDRGFF
                     PTYDAGLLTRDDMPAPVAAVLSRLESRIDEETMQGLNRRVASQGEEPARVAADYLRET
                     GLVSAADAVAVAGYERILGNTLVHLKLTGIALLLGCLVAVPLALVVARHQRIASGLLY
                     VTGLVQTVPSLALLALLIPLVGLGQLPAIIALFLYSLLPIVRNTLTGLFNVDPLLKEV
                     AGSMGLTTAQQLRKVELPLAMPTILAGIKTAAIISIGTATLAAFVGAGGLGEPIITGL
                     TLNDHALILEGAVPAALLAVVVELLFELIERATVPAHLRVS"
     gene            2255..>3380
                     /gene="egtB"
                     /locus_tag="AC16PP_13920"
     CDS             2255..>3380
                     /gene="egtB"
                     /locus_tag="AC16PP_13920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HIR6482772.1"
                     /translation="MGKALAIDATAAAFSDTRRQSVDVCRPLEIEDYGLQAAVFASPP
                     KWHLAHTTWFFETFILKPFVSGYAPVEPIYEVLFNSYYNGIGEQHPRHQRGLLSRPTL
                     DEVLAYREHVDAAMAGCLELAGRADPQAIRQRTVLGIEHERQHQELFFTDLKYSLSVN
                     PLYPAYLAAELPVQSGTPVRWLGCEGGLVEIGHDGSGFCFDNELPRHRCFVEPFEIAS
                     RLVTNEEYQAFVDDDGYGRPELWLADGWTTLQSEGWKAPLHWLERDGQALEFTLHGLQ
                     PRRGSRPVCHVSGYEADAFARWANARLPTEQEWETVAAGHPAGAAGMDDGVYHPQAAP
                     ADEGPTQLYGACWQWTRSAYGPYPGYSASPGAIGEYNGKFM"
BASE COUNT          524 a         1114 c         1154 g          588 t
ORIGIN      
        1 tcgtcggccg cagcgggtgc ggcaagtcca ccttgttgaa gctcctgaac gggctggtgg
       61 tgcccgacgc aggccgagtc tccgcgttcg gaacccagct ggattacgct gatctggtat
      121 ccgtgcgccg gcggatgggt tacgccgtgc agggcacggg cctctgtccc cacatgagtg
      181 ccagggaaaa cgtcgttctc tcggcggaga ttgcgggctg gaaagagcag gcggtggctc
      241 aacggcttgg cgagctgctc gagcttacgc gcctttccgc tgagctcctc gccaagtacc
      301 cccacgagct cagtggcggg cagcagcagc gcgtgggcct gtgccgggcc atgatgctca
      361 ggcccgaggt tctgctgctg gatgagccgt ttgcggccat cgatcccatc acccgctacg
      421 acatccacca tcagctgctg gagattctcg ccgcggagcc cgccaccgtc gttctggtca
      481 cccacgacat gcgggaagcc atgcagctgg cggaccgcat cgtcctgctg gaaggaggcc
      541 gggtgtttct cgatgagtcc cgggactccc ttgcccgctc caggccgggc gtggagcccg
      601 aggctttgct gcagtcgctg atggaggagg cgctggcatg aagggagcat gcctgctcct
      661 cgcggctgcc tgcatcgtcc ttgtggcacc tgtgcgagcg gccgagcggg tcaccgtggc
      721 atccaaagcg ttcagcgaaa gctacgtgct cgcggagatc gccgcccagc tgctggaggc
      781 ggaaggcttc gaggtggaac gcaagctggg ccttggcggc acgctgattg ccttcggcgc
      841 gcttcgggag ggcgccgtgg acgtatatcc cgagtacacg ggtaccctga cccaggcgat
      901 actcgacgag ccgggcatga ccattgcgga gctcgacgcc gctctggccg agcgcgggct
      961 cgttttccgg gtccctctcg ggttcaacaa cagctacgcc gtggccgtca ccgaagagat
     1021 ggccggtgcc cggggcttaa cgcgggtgag cgacctggcc gggcagacgg atctcagctt
     1081 cggcttctcc cacgagtttc tcaatcgcgc cgacggctgg cctgcgctca agacggccta
     1141 cggcctgacc cagacggcat ccggaatcga gcacgccctg gcctaccgcg ccctggcgag
     1201 cgggcatctc gacgtgaccg atgcctacac cacggatggg gagctttcgc tctacgcgct
     1261 tcgcctgctg gcagacgacc gcggcttctt tcccacctac gacgccgggc tgctgacccg
     1321 tgacgacatg cccgcgcccg tggccgccgt gctgtcgcgg cttgaaagcc gtatcgacga
     1381 ggagaccatg caggggctga accggcgcgt ggccagccag ggcgaggagc ctgcccgcgt
     1441 agcggccgac tacctccggg aaacggggct ggtgtcggcg gctgatgccg tggccgtggc
     1501 cgggtacgag cggatactgg gcaacaccct cgttcacctg aagctcaccg gcattgcgct
     1561 gcttttgggt tgcctcgtgg ccgtgcccct ggcccttgtc gtcgcgcggc accagcgcat
     1621 cgccagcggc ctgctgtacg tgacgggcct ggtgcagacc gtgccttccc ttgcgctgct
     1681 ggcgttgctc atcccgctcg ttggcctggg ccagctgccg gccatcatcg cgctgtttct
     1741 ctactccctg ctgcccatcg tgcgcaacac gctcaccggg ctgttcaacg tcgacccgct
     1801 gctcaaggag gtggccggga gcatggggct gaccacggcg cagcagctca ggaaggtgga
     1861 gctgcccctg gccatgccca ccatcctcgc gggcatcaag accgctgcga tcatcagcat
     1921 cgggacggcc accctggccg ccttcgtagg cgccggcggg ctcggcgagc cgatcatcac
     1981 cggtctcacg ctgaacgatc acgcgctgat tctggaaggc gcggttcctg ccgctttgct
     2041 ggccgtcgtc gtggagctgc tcttcgagct catcgagcgg gcgacggttc ccgcacatct
     2101 gcgggtttcc tgacgatgtc gtcaaagtcc ggtccgaagc tgttaggctt tagaggagtc
     2161 ggaggcggcg ggcctatggc ctgacccccc ggaatggcgc acgctcagcg gcgcgcggct
     2221 cggatctgcg ggccgggagg ggcagacgat caatatggga aaggctttgg caatcgacgc
     2281 gacggcggcc gcgttcagcg atacccggcg acaatccgtg gacgtctgcc ggccgttgga
     2341 gatcgaggac tacggcctgc aggccgccgt gttcgcaagc cctccgaagt ggcatctcgc
     2401 tcataccacc tggtttttcg agaccttcat cctcaagccg ttcgtctcgg gctacgcgcc
     2461 cgtcgagccc atctacgagg tgctcttcaa ctcttactac aacgggatcg gcgagcagca
     2521 tccgcggcat cagcgggggt tgctatcgcg ccccacgctg gatgaggtgc tcgcctaccg
     2581 ggagcacgtg gacgcagcga tggcaggctg tctggaactg gccggacggg ccgatccgca
     2641 ggccatacgc cagcgcaccg ttctgggcat cgagcacgag cgccagcacc aggagctctt
     2701 cttcacggac ctcaaatact cgctgagcgt caacccgctg tacccggcct acctcgccgc
     2761 cgagctgccg gtgcagagcg ggacgccggt gcggtggctt ggctgcgagg gcggcctcgt
     2821 cgagatcggc catgacggaa gcggcttctg cttcgacaac gagctgcctc gacatcgatg
     2881 tttcgtggag cctttcgaga tcgcctccag gctcgtcacc aacgaggagt accaggcgtt
     2941 cgtggacgac gacggctacg ggcgtccgga gctgtggctg gccgacggct ggacgacgtt
     3001 gcagagtgaa ggatggaaag cgccgctgca ttggctcgaa cgtgacggcc aggcgctgga
     3061 gttcaccctg cacggcctgc agccgaggcg ggggtcgcgg cccgtctgtc atgtgagcgg
     3121 ctacgaggcc gatgctttcg cccgctgggc gaacgcgcgg ctgccgaccg agcaggagtg
     3181 ggaaacggtg gcggccggac atcccgccgg agcggcgggc atggacgacg gcgtctacca
     3241 ccctcaggca gctcctgcag atgaaggccc gacgcagctg tacggcgcgt gctggcagtg
     3301 gacgcggagc gcgtacggtc cgtacccggg ttacagcgct tcgcccggcg ccatcggcga
     3361 gtacaacggc aagttcatga
//
LOCUS       DBNHKV010000427         5925 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1336308, whole genome shotgun sequence.
ACCESSION   DBNHKV010000427 DBNHKV010000000
VERSION     DBNHKV010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5925)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1336308"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="AC16PP_13925"
     CDS             <1..750
                     /locus_tag="AC16PP_13925"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6482773.1"
                     /translation="TADLRSAIAAGVLADLAGSCGDEAAVPLDGLTYLPTIPNPDKIL
                     CVGLNYKAHQEETGRGGEGFPTIFVRFATAQIGHEQPMIRPTESATLDYEGEIALVIG
                     IPGRRISRSDALDHVAGVSIYNDGSVREYQRQTSQFTAGKNFVGTGGFGPWMMTPDEV
                     GDLNSMEITTRLNGEVMQNATADLLVHGFAELIEYCSTFIELLPGDVIVTGTPGGVGV
                     ARNPPVFMDEGDVVEVEVKPVGVLRHRVVVG"
     gene            816..2345
                     /locus_tag="AC16PP_13930"
     CDS             816..2345
                     /locus_tag="AC16PP_13930"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR6482774.1"
                     /translation="MPDRRGRSRILSGACWCLLILCVSGCVYLAQPEDAVVSQAQAPD
                     PQSRIGQLLGGGRGVPARGLLVEASDLALTARIDMIDAADATIDAQYFIWQNDPTGVL
                     VIQKLLDAADRGVRVRALLDDVQLTGFVDRLNALDDHPNIEIRIFNPFSVRFRYPLGF
                     IRIAEFAIDGARLNHRMHNKLMVADNQLAILGGRNIGDDYFGRNPKRNFVDTDILLSG
                     DIVPELSRGFDSYWNSRWAYPVSALMEFSLLPVDLETIRQRIRDRLEEWPDLESLGRE
                     IAFEDTLADLRDAAALDWSTTVVDDPEVGWFERPDDIALDLTELALRAEREVLVVTPY
                     LIPTPNAFKIGKELVDKGVRVRIVTNSLGTNDVVAAQAAYGRYRKRILDTGVELYELR
                     ADAALPERGLSEEVCLHSKYIIIDDEIVFVGSLNLDPRSLYLNTEVGVVLESRPLAAT
                     LRESFDLLAHPDSSWRVTTHENGFRWESSAGTLDRQPAKNGWQRFRYWFLSLIPVSSQ
                     L"
     gene            2481..3599
                     /locus_tag="AC16PP_13935"
     CDS             2481..3599
                     /locus_tag="AC16PP_13935"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6482775.1"
                     /translation="MSYPIISADSHITEHPDTYSAHIDPAWRDRAPKMIDGGERGDLF
                     VIDGMDTPIAMGLVAAAGKPAEELTMAGVRFEELHRSGWDPAARMADQDRDGVAAEII
                     YPTVGMLLCNHRDFDYKKACFDAYNRWIAEYCSTNPARLLGCGQTAVRSPAETVSDLE
                     KIRELGLRGVMMPGDPLEEDYDSQIYNPVWEAAIDLGLPLSFHILTTRQQAPVRGPRI
                     NSFLSIIRGNQDIMGTLIFGGVFDRYPELKVVCVEADAGWVPHYMYRMDHAYKRHRNW
                     LAKGVSLARLPSEYFSEHVYTTFQDDWVAFRLVNDMNWRRLLWANDFPHSDSTWPWSQ
                     QMLAEQTANLTEEQKRGILCDNVAELYRIDVDALAQAA"
     gene            3599..3922
                     /locus_tag="AC16PP_13940"
     CDS             3599..3922
                     /locus_tag="AC16PP_13940"
                     /inference="COORDINATES: protein motif:HMM:NF049702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7508 domain-containing protein"
                     /protein_id="HIR6482776.1"
                     /translation="MSIRLTKPWSPASAAGKLPGQMGVYQLGDEAGNVITIGYAGGRS
                     TFGLRGEVETALQRHPDAVSYRVEVNSAYLSRYRELLMVHLADFGTLPPGNEALPGLG
                     RLSPA"
     gene            3932..5098
                     /locus_tag="AC16PP_13945"
     CDS             3932..5098
                     /locus_tag="AC16PP_13945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6482777.1"
                     /translation="MDLDLSEEQNLLIGTVRRFVREEILPLEQSLDPDADELEPDDHE
                     RLTQLTREMGLYGLDVPPEFGGPDVDLVTRTLLAMEMSQHRAGLYAPCYGVFGGAGLA
                     QLFEATEDQKDRYLFPLLRGEKKGFFGLTEPSGGSDPARAIQTRAVRDGDEWVVNGSK
                     IFISGADRADFGLVFARTDADKGRNGVTCFIVDTDTPGFHVRRIVHTLRTARYATELS
                     FEDMRVAHGNVLGEVNGGFAIANDRLSRQRIPYAAGCIGVAIKAHEMALEYVPQRETF
                     GAPLSTRQAIQWMLVDNEIDIRQASWLTLEAAHKADKGEPFRKEAAMAKLVATEAGSR
                     VVDRCMQMFGGYGVTKDFPFERWYREMRIRRIGEGPNEVQRHIIARDILGASLR"
     gene            5170..>5925
                     /locus_tag="AC16PP_13950"
     CDS             5170..>5925
                     /locus_tag="AC16PP_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482778.1"
                     /translation="MLQGRYSEAELYSEHDYARPHQIAGRRLHGGFDAAGNYVPPRSK
                     GRSEAIEAWTRALRERGGELFDADASLLSGPRMPTVEQQHMLLREGIGRPFWNTLTVT
                     GKIEGRGRVLAEMQFPDLQDVVVEDISDKAVGHLNRGLLAIHGIDEGGQPELGLGGHD
                     VMWFVARDLVFGPGAYPDVEPPESIARPEAGKRWMPEISAEHEALLSFLMNLLIIEFR
                     AEIGFAATQDILRADDLFTDRRAQACEAAEIIER"
BASE COUNT         1017 a         1903 c         1991 g         1014 t
ORIGIN      
        1 accgccgatc tgagatcggc catcgcggcc ggtgtcctgg ccgatctggc cggctcctgc
       61 ggcgacgagg cggccgtccc cctcgacggg ctgacctacc tgccgaccat cccgaacccg
      121 gacaagattc tctgcgttgg gctgaactac aaagcgcatc aggaggagac gggtcggggc
      181 ggcgaaggat tccccacgat cttcgtgcgc ttcgcgaccg cccagatcgg gcacgagcag
      241 ccgatgatcc gtccgaccga atcggcaacg ctggactacg aaggggagat cgccctcgtg
      301 atcggaatac ccggtcgacg catatcccgc tcggatgccc tcgatcacgt ggcgggggtc
      361 agcatctaca atgatggcag cgtgcgcgag taccagcgtc agacgtccca gttcaccgca
      421 ggcaagaact ttgtcgggac gggcgggttc ggcccctgga tgatgacacc ggacgaggtc
      481 ggcgatctga acagcatgga gatcaccacg cgcctgaacg gggaggtcat gcagaacgca
      541 acggccgatc tgctcgtgca cgggttcgcg gagctcatcg agtactgctc caccttcatc
      601 gagcttctgc ccggggacgt gatcgtcacc ggcacgccgg gcggggtggg tgttgcccgc
      661 aacccgccgg ttttcatgga cgagggcgac gtggtcgagg tggaggtcaa acccgttggc
      721 gtgctgcggc atcgggtggt tgtgggctga gccggtcgcc accgcaccgg cgagtggccg
      781 cggtccatcc gtgattctga ctgctggccg gacccgtgcc tgatcgtcgc ggccgctcga
      841 gaattctctc cggcgcctgc tggtgcctgc tcatcctctg cgtatccggg tgcgtctacc
      901 tggcgcagcc ggaggatgcc gtggtcagtc aggcccaggc gcccgatccg caatcccgga
      961 tcggtcagct tctcggcggc gggcgcgggg tgcccgctcg gggactgctg gtagaggcct
     1021 ccgacctggc gctgaccgcc cgcatcgaca tgatcgacgc ggcggacgcg accatcgatg
     1081 cgcagtactt catctggcag aacgacccga cgggcgttct ggtaatccag aagctgctgg
     1141 acgcggcgga ccggggggtt cgggtacgcg cgctgctaga cgacgtgcag ctcaccgggt
     1201 tcgtcgaccg gctgaacgcg ctggacgacc atccgaacat cgagatccgg atcttcaatc
     1261 ccttcagcgt gcgctttcgc tacccgctgg gtttcatccg catcgccgag ttcgccatcg
     1321 acggcgcgcg gctgaatcat cggatgcaca acaagctcat ggtcgccgac aaccagctgg
     1381 ccatcctcgg cggtcgcaac atcggcgacg actattttgg caggaacccg aagcgcaact
     1441 tcgtcgacac ggacatcctg ttgtccggcg acatcgttcc ggagctgtcc cggggattcg
     1501 attcctactg gaacagccgc tgggcttatc ccgtgagcgc gctgatggag ttctccctgc
     1561 tgccggtgga tctggagacg atacgtcagc ggatccgtga ccgcctcgag gagtggcccg
     1621 atctggagtc gctcggccgc gagatcgcgt tcgaggacac ccttgccgat ctgcgcgacg
     1681 ctgcggcgct ggactggtcg accacggtgg tcgacgaccc cgaggtcggc tggttcgaac
     1741 gcccggacga cattgccctg gatcttacgg agctcgcgct gcgtgccgag cgcgaggtgc
     1801 tggtggttac tccttacctg atccccacgc ccaacgcctt caagatcgga aaggagctcg
     1861 tcgacaaggg ggtgagggtg aggatcgtca ccaactccct gggaaccaac gacgtggtcg
     1921 cggcccaggc ggcctacggg cgctaccgca aacgcatcct ggatacgggc gtcgagcttt
     1981 acgagctgcg ggcagacgcg gcgttgccgg agcgtgggct ctccgaggag gtctgcctgc
     2041 actccaagta catcatcatc gatgacgaga tcgtcttcgt gggctcgctg aacttggatc
     2101 ccaggtccct ctacctgaac acggaggtcg gggtggtgct ggagtcgcga cctctggcgg
     2161 cgacgctgcg agagtccttc gatctgctcg cccatcccga cagctcatgg cgggtcacga
     2221 cccacgagaa cggattccgc tgggagtcgt cggccggaac gctcgatcgc cagcctgcaa
     2281 agaacggctg gcagcgtttc cgctactggt tcttgagcct catcccggtc tccagccagc
     2341 tctgaggcct ggggttcggg gctttcgccg cgcgcggcga aggggcgcgg gcggcccgct
     2401 gatccgtccg cggcgccttg acaggcagcg ccgtcggccg ggatagtcgg tggcgtgatc
     2461 gtcatcaaag gggagagacg atgagctatc cgatcatctc ggccgattcc cacatcaccg
     2521 agcatccgga cacctactcg gcgcacatcg atccggcctg gcgggatcgc gctccgaaaa
     2581 tgatcgacgg cggtgagcgg ggcgacctct tcgtcatcga cgggatggac accccgattg
     2641 ccatggggct ggtggctgcg gctggaaagc ctgcggagga actcaccatg gcgggtgtgc
     2701 gcttcgagga gctgcaccgc agcggctggg atccggcagc ccgcatggcg gatcaggacc
     2761 gggacggggt agccgcggag atcatctacc cgaccgttgg catgcttctg tgcaaccatc
     2821 gggatttcga ctacaagaag gcctgcttcg acgcttacaa ccgctggata gccgaatact
     2881 gctcgacgaa cccggcccgc ctcctgggct gcggccagac ggccgtgcgc tcgccggcgg
     2941 agacggtgtc cgatctggag aagattcgcg agcttggcct gcgcggcgtg atgatgcccg
     3001 gagacccgct ggaggaagac tacgactcgc agatctacaa cccggtctgg gaagctgcca
     3061 tcgacctcgg tctgccgctt tcgtttcaca tcctcacgac ccgccagcag gctccggtca
     3121 ggggtccgcg catcaacagc tttctttcga tcatccgggg aaaccaggac atcatgggca
     3181 ccctcatctt cggcggcgtc ttcgaccgct atccggagct caaggtggtc tgcgtggagg
     3241 cggacgccgg ctgggtgccc cactacatgt accggatgga ccacgcctac aagcgtcacc
     3301 gcaactggct ggccaagggc gtcagcctgg ccaggcttcc gtccgagtac ttcagcgagc
     3361 acgtctacac cacctttcag gacgactggg tggcgttcag gctggtcaac gacatgaact
     3421 ggcggcgact gctctgggcc aacgattttc cccacagcga ctcgacctgg ccgtggtccc
     3481 agcagatgct cgcagagcag acggcgaacc tgaccgagga gcagaaacgg gggatcctct
     3541 gcgacaacgt ggcggaactc taccgcatcg atgtggacgc cctcgcccag gccgcctgat
     3601 gagcatacgg ctgaccaagc cctggtcgcc cgccagcgcc gctgggaagc tcccgggcca
     3661 gatgggcgtc tatcagctgg gcgacgaggc gggcaacgtc atcaccatcg gctacgccgg
     3721 cgggcgcagc acgttcggcc tgcgcggcga ggtcgagact gctcttcagc gtcaccctga
     3781 tgccgtgagc taccgcgttg aggtcaactc ggcctacctg tcgcgatatc gtgagctgct
     3841 catggtgcac ctggcggatt tcgggacgct gccgcccggc aacgaggccc tcccggggct
     3901 cggccggctc agcccggctt gaggggtagc catggatctc gatctgagcg aggagcagaa
     3961 cctgctgatc ggcacggtgc ggcgcttcgt tcgggaggag atcctgcccc tggagcagtc
     4021 tctggatccg gatgcggacg agctcgagcc cgatgatcac gaacgcctga cgcagctgac
     4081 ccgcgaaatg gggctctacg gcctggacgt cccgccggag ttcggcggcc cggacgtcga
     4141 tctggtgacc cggacgctgc tcgccatgga gatgagccag catcgcgccg gtctctacgc
     4201 gccgtgctac ggggtctttg gcggtgcggg gctcgctcag ctgttcgaag cgacggagga
     4261 tcagaaggat cggtacctct tccccctgct gcgcggggag aagaagggtt tcttcggcct
     4321 cactgaacct tcgggcggca gcgatcccgc acgagcgatt cagaccaggg ccgttcgcga
     4381 cggcgacgag tgggtcgtca atggcagcaa gatcttcatc agcggtgctg accgggcgga
     4441 tttcggcctc gtgtttgcgc gcacggatgc cgacaaggga cgcaacgggg tcacctgctt
     4501 catcgtggat acggacacgc cgggctttca cgttcggcgc atcgttcaca cgctgcgcac
     4561 ggcccgctac gccaccgagc tctccttcga ggacatgagg gtggcccacg gcaacgtgct
     4621 cggagaggta aacgggggct tcgccatcgc caacgatcgg ctctccagac agcgcatacc
     4681 ctatgccgcc ggttgcatcg gcgtcgcgat caaggctcac gagatggccc tggaatacgt
     4741 tccccagcgc gagacgttcg gcgccccgct gtccacacgt caggccattc agtggatgct
     4801 ggtggacaac gagatcgaca tacggcaggc ctcctggctc accctggagg ccgcgcataa
     4861 ggccgacaag ggcgagccgt tccgtaagga ggcggccatg gccaagctgg tggccaccga
     4921 ggcgggctcc cgggtggtgg accggtgcat gcagatgttc ggcggctacg gggtgaccaa
     4981 ggacttcccc ttcgagcgct ggtatcggga aatgcgcatc cggcgaatcg gcgagggccc
     5041 caacgaggtg cagcgacaca tcattgcccg ggacattctg ggggcgtctc tgcgctgaaa
     5101 cgctttgccg ggacggcttc cggcgcaatc tgttataacc ggcgctcctg ggacgctacg
     5161 aggctgcaaa tgctacaggg acgctacagt gaggccgagc tctacagcga gcacgactac
     5221 gcgcggccac accagatcgc cgggcgccgc ctgcacgggg gctttgacgc cgccggcaac
     5281 tacgtgccgc cccgttccaa ggggcgctcg gaggccatcg aggcgtggac ccgggcgctg
     5341 cgcgaacgcg gcggagagct gttcgatgcc gatgcgagcc tgctttcggg cccgcgaatg
     5401 cccaccgtcg agcagcagca catgctgctc cgcgaaggca tcggccggcc gttctggaac
     5461 accctgaccg tgaccggcaa gatcgagggg cgcggccggg tgctcgccga gatgcagttc
     5521 cccgatcttc aggacgtcgt ggtggaagac atctccgaca aagccgtcgg tcacctcaac
     5581 cgcgggctgc tcgccattca tggcatagac gaaggcggac agccggagct tggcctgggc
     5641 gggcacgacg tgatgtggtt cgtggcccgc gacctggttt tcggccccgg ggcatacccg
     5701 gacgtggagc cgccggaaag catcgcccgg cccgaggccg ggaaacggtg gatgcctgag
     5761 atatccgctg agcatgaggc gctgctcagc tttctgatga acctgctcat catcgagttt
     5821 cgggcggaaa tcgggtttgc cgcaactcag gacatcctgc gggcagacga cctgttcact
     5881 gaccggcgcg cgcaggcttg cgaggcggcg gagatcatcg agcgc
//
LOCUS       DBNHKV010000428         7428 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1478906, whole genome shotgun sequence.
ACCESSION   DBNHKV010000428 DBNHKV010000000
VERSION     DBNHKV010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7428)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1478906"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1699)
                     /locus_tag="AC16PP_13955"
     CDS             complement(62..1699)
                     /locus_tag="AC16PP_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HIR6482779.1"
                     /translation="MAEEPNDFIRQRIRADLASGVVKGGVVTRFPPEPNGHLHIGHAK
                     SICLNFGVAEEFGGYTYLRFDDTNPLKESEEFALAIQEDVRWLGYDWGDHLTHASDYF
                     EELYGFAVQLIEQGKAYVCSLTGDEIRATRGSLTEPGTESPYRDRSVEENLNLFRRMR
                     EGEFADGEHVLRAKIDMASPNINMRDPVIYRIRHASHQRTGDAWVIYPMYDFTHCICD
                     ALEGITHSLCTLEFEDHRPLYDWVLDNININFHPPQIEFSRLGLEYTVMSKRLLHRLV
                     AEGLVEGWDDPRMPTIAGLRRRGVTPGAIREFCRRIGVTKQDGMVEMNLLEFCIRQDL
                     ESSAPRGMAVLDPLKVVITNHPGAEEYLVAPWHPNEAELGERELPFGPEIYIERADFM
                     EEPPRKYKRLSPNEMVRLRYAYVIRCDEVVRDEVGNIAELRCTYFSDSKSGADTSGLK
                     PKGVIHWVSAATGMPTRIRLYDRLFTVPAPRGEELVGELNTQSLAEMSGFVEPAVAAS
                     AARRFQFERQGYFCKDSQLDGVFNRTVTLRDSWKPAP"
     gene            1797..3200
                     /locus_tag="AC16PP_13960"
     CDS             1797..3200
                     /locus_tag="AC16PP_13960"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR6482780.1"
                     /translation="MTLRLIVAGAICIALGAGSVLLEQHASQAVQSARHFLGERWYVL
                     ELGGRQVGYLHTGASHDREGQWQFRSELRFALQPDEAVTVEDELTFAAVAPHPLSEAT
                     HRISQPGSTQLVVIRRDATHYSAIVSSTEGERGEPTARLDWSYALGDYLAFETWLRTA
                     SPPPGAARTVPTLNFSRLAPFAKAYRIAERNPTGYRVSSPDPLDETIIQLDDDHLPVT
                     LEMAGLFKLRRTSRDLAVGGRSPLKAATYYVPADQRLPNHTQIETIVLGVHGAPDARR
                     LWPQAEGRSGSWTLTLNANPVSSDADPRDHTATTLEFPATDERIVELARTAVAGTSGQ
                     AERVAALTRFVHHYLTYAPGGASRPVLALLDEPVGDCTEHADLFTTLARSLNIPARTV
                     FGLAYADREEPAFAFHAWNEVSLDETWRPVDPTWNLLRVDATHIPLPSNRGAALALIT
                     GATKLSFSVLEVGYFDD"
     gene            complement(3197..4093)
                     /locus_tag="AC16PP_13965"
     CDS             complement(3197..4093)
                     /locus_tag="AC16PP_13965"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF044908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6482781.1"
                     /translation="MHAMTQDTDQSRRAARLLEVLDLECIEQNLFLGANEVRGEFRLF
                     GGQVLSQSLRAACLTVEGRSAHSLHGYFMRAGNAGRPVLYEVDRIRDGRSFATRRVVA
                     IQSGEAIFSLDVSFQVDEAGFEHAHPMPNVPPPEELEGDIAVVSRLTGDDPHLSPMAG
                     RPRPFELRSVFKPGSDEWAANRHWNPVWIRFAGEVDPDDDPLARSLLAYASDVGLVST
                     GVLPHVESISRAEVRMASLDHALWFHRAVPIADWLLVHKRTTMARGGRALIQAEIFSR
                     DGKLVASVSQEGLIRRRSAAPG"
     gene            4505..6334
                     /locus_tag="AC16PP_13970"
     CDS             4505..6334
                     /locus_tag="AC16PP_13970"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6482782.1"
                     /translation="MAARQSINLWQLTRGQRLRYAGAIFAMGMTNLFIFGPVLVSGSA
                     IDVIADQDLSHAVPPLLWAASRLSEGAPFAAYLWVAAVVAVTMTAIGGAFLFARGRFA
                     AVASEAIVRNLRERLYDRLHDLEAGFFDTADTGDLVQRASSDVETLRVFLASDVVEIG
                     RAIMLIMCVAPILAWLDVRLAALSLCLMPLLAIGAYLFFARVKDVFQITDESEAAMTA
                     TLQENLTGIRVVRAFARQAFEIEKFGARNAEFRNNNRRLIQLMGIYWSTSDLFAMTQI
                     GIVLIAGGLFVLDGSITVGTLFEFMTMVSMVIWPVRQLGRVLTDTGKAVVSLGRINEV
                     LTEAEERAGTIPGEGRARGEIRIENLTFGYDPQRPVLEDVNLHVEVGETLAIVGPPGS
                     GKSSLIRVLLRMYPYQKGSVRIDGWEVAELDRLWLRHQIGVVLQDPFLYSRSIRANLA
                     LGRPGAPENELIEACHDAAVHDAIIGFPEGFEAMVGERGVTLSGGQRQRMALARALLK
                     DPPILVLDDSLSAVDTGTERRILDAMRRRKGRQTTLIIAHRLSSVMHADRILVLQEGR
                     VVQLGNHATLAGEPGPYRTLCEIQGALDASIEADTNATGRGNG"
     gene            6327..>7428
                     /locus_tag="AC16PP_13975"
     CDS             6327..>7428
                     /locus_tag="AC16PP_13975"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR6482783.1"
                     /translation="MDNYDDENFDDGFDDEALAARVDLKLWRKLFAYARRYPKDLVWL
                     AVCAFTTASMEVTYPLITKWVVDDVDAAYAAGTVISLWPHALAYLACTVTICIAIGGF
                     IWMGGKIRTHVSHDIRRDGFENLQRLSFAFYDYRPVGWLMARMTSDCERLSNILAWGF
                     LDLVWGSTMMLGIAVAMLVMNPKLALVVLAILPLLGWVSARFQRRILSSARRVRATNS
                     RITASFNEAIMGVLTSKAFVREQANLDEFRELTNTMHGASVLNLSQAAVYVPIVITLA
                     SLATGLALAIGGLDLLGGAIAIGTLVAFMAYTRHFFDPIEQLGHWFAEMQMAQASAER
                     ILSLIETDPAVKDSPDVIEALQRQADAPPRRNY"
BASE COUNT         1266 a         2534 c         2344 g         1284 t
ORIGIN      
        1 ggtcggcttc tgacatttac gtgacctcct ggttatgccc tgaacggacc accgcgacgg
       61 cctaaggagc gggcttccag ctgtcccgga gcgtcaccgt gcggttgaaa accccatcga
      121 gctgagagtc cttgcagaaa tagccctgtc gctcgaactg aaaccttctc gcggcgctcg
      181 ccgccaccgc cggttcaacg aaaccggaca tctcggccag cgactgcgta ttgagctcgc
      241 cgacgagctc ctcgccacgc ggcgcgggga cggtgaagag ccgatcgtac agcctgattc
      301 gtgttggcat gcccgtggcc gccgataccc agtgaatgac gcctttgggc ttgaggccgc
      361 tggtgtccgc gccgctcttc gagtcggaga aataggtgca gcgcagctcg gcgatgtttc
      421 ccacctcgtc cctgaccacc tcgtcgcacc ggatcacgta ggcgtagcgc aggcgcacca
      481 tctcgttggg agacagacgc ttgtacttcc tcggcggttc ctccatgaag tcggcgcgct
      541 cgatgtatat ctccgggccg aacggcagct cgcgctcgcc caactctgcc tcgttgggat
      601 gccacggcgc caccagatac tcctcggcgc ccgggtggtt ggtgatcacc accttgagcg
      661 ggtccaaaac ggccatgcct cggggcgcgg agctttccag atcctgtcgg atgcagaact
      721 ccagcaggtt catctccacc ataccgtcct gcttggtcac cccgatacgg cggcagaact
      781 cgcgaattgc tccaggggtc accccacgac gcctgagacc cgcgatggtc ggcatgcgcg
      841 ggtcgtccca gccttccacc agcccttccg ccaccaggcg gtgcaggaga cgcttgctca
      901 tgaccgtgta ctcgagcccc aggcgggaaa actcgatctg cggcgggtga aagttgatgt
      961 tgatgttgtc cagcacccag tcgtagaggg gccggtggtc ctcgaattcc agcgtgcaaa
     1021 gcgagtgcgt gatcccttcc agggcatcgc agatgcagtg cgtgaagtcg tacatcgggt
     1081 agatcaccca ggcatcaccc gttcgctgat gagaagcatg gcggatccgg tagatcacgg
     1141 gatcgcgcat gttgatgttc ggggacgcca tgtcgatctt cgcccgcaga acgtgctcac
     1201 cgtccgcaaa ctcgccttcg cgcatgcgcc ggaagagatt gaggttctcc tccacggatc
     1261 ggtcgcgata gggactctcg gtgccgggct cggtgaggga cccccgggtc gcccggatct
     1321 cgtcaccggt caggctgcag acgtaggcct tgccctgctc gatcagctgc acggcaaagc
     1381 cgtagagctc ctcgaaatag tccgaggcgt gggtgaggtg gtcgccccag tcgtaaccca
     1441 gccagcgcac gtcctcctga atggccaggg cgaactcctc gctctccttg agcgggttgg
     1501 tgtcgtcgaa gcgaagatag gtgtagcccc cgaactcctc ggcaaccccg aagttcaggc
     1561 agatggactt ggcgtggccg atgtgcagat gcccgttcgg ctccggcgga aatcgagtca
     1621 ccacgccgcc cttcacgacg ccgctggcaa ggtccgcgcg aatgcgctgg cgtatgaagt
     1681 cgttgggttc ttcggccatc ggcccatcta cggaatctgc ggggcgcgta ttctaaccaa
     1741 aggccgaaac cggcgtctgc tgctaatatt cttcgggaaa gcacaggggg agcttcgtga
     1801 ctttacggtt gatcgtcgcc ggcgcgatct gcatcgcgct cggcgcggga tcggtcctgc
     1861 tcgagcaaca tgcctcccaa gccgtccaga gcgcccgtca cttcctcggt gaacgctggt
     1921 acgtcctgga gctcggcggt cgccaggtcg gctacctgca caccggcgcc agccacgatc
     1981 gcgagggcca atggcagttc cgcagcgagc tgcgattcgc gctgcagccc gacgaggcgg
     2041 tcaccgtcga agacgagctg accttcgctg ccgtggcacc tcatcctctc tccgaggcca
     2101 ctcaccgcat cagccagcca ggcagcacac agctcgtcgt catcaggaga gacgcgacgc
     2161 actactcggc gatcgtctcg tcgactgaag gggaacgcgg cgaacccacg gccaggctgg
     2221 actggtctta cgcgctcggc gactacctgg ctttcgagac ctggctgagg accgcctcgc
     2281 cgcccccagg cgccgccagg accgtgccca ccttgaattt ctcccggctc gcgccgtttg
     2341 ccaaggcgta ccggatcgcg gagcgaaacc cgacagggta tcgcgtgtcc agccccgacc
     2401 ctctggatga gaccatcatc cagctcgatg acgaccacct gcccgtcacc ctggaaatgg
     2461 ccggactgtt caagctgcgc cgcaccagcc gggacctggc cgtgggcggg cgcagccctc
     2521 tgaaggccgc cacctactac gttcctgcag accagcgact gcccaaccac acccagatcg
     2581 agacgatcgt tctcggcgtg cacggcgccc cagacgcccg ccgtctctgg ccgcaggccg
     2641 agggccgatc ggggagctgg acactgacgc tgaatgccaa cccggtgtca tccgacgcgg
     2701 atccacgcga tcacaccgca acgacgctag agtttccggc cactgacgag cgcatcgtcg
     2761 agctcgcgcg caccgcggtc gcgggtacga gcgggcaagc tgagcgggtg gccgcgctca
     2821 cccggttcgt ccatcactac ctgacctacg cgccgggcgg ggcttcgcgt cccgtgctgg
     2881 cgctgctcga cgaaccggtc ggcgactgca cggagcacgc cgacctcttc accaccctgg
     2941 cccgcagcct gaacattccc gcccggaccg tcttcggtct ggcctacgcc gatcgggagg
     3001 agcccgcgtt cgccttccac gcgtggaacg aggtgtcctt ggacgagacg tggcggccgg
     3061 tggacccgac atggaacctg cttcgcgtcg acgccaccca cataccgctg cccagcaatc
     3121 gcggggcggc cctggccctg atcacgggcg caacgaaact cagcttttcc gtgctcgagg
     3181 tcggctactt cgacgactag ccgggggcgg cgctgcggcg tctgatcaga ccctcctggg
     3241 atacggaagc gaccagcttg ccgtctcgtg aaaatatctc cgcctgaatg agcgcccgcc
     3301 cgccccgggc catggtcgtc cttttgtgaa cgagcagcca gtcggcgatg ggcacggccc
     3361 gatgaaacca cagcgcgtgg tccaggctcg ccatgcgcac ctcggcgcgg ctgatcgact
     3421 ccacatgggg aaggaccccg gtcgagacca ggcccacgtc ggaggcgtag gcgagcagcg
     3481 accgggccag cgggtcatcg tcgggatcca cttcgcccgc gaagcggatc cacacggggt
     3541 tccagtgccg gtttgccgcc cactcgtcgc tgcccggctt gaacaccgag cgaagctcga
     3601 acggccgcgg ccggccggcc atggggctca agtgcggatc gtctcccgtc agccggctca
     3661 ccacggcgat gtccccctcc agctcctcag ggggcggcac gttgggcatg gggtgcgcgt
     3721 gctcgaatcc cgcctcgtcg acctggaagg agacgtccag gctgaagatc gcctcccccg
     3781 attggatggc caccacgcgt cgggtagcaa agctcctgcc gtcgcgaatg cggtccacct
     3841 cgtagagcac gggtctcccc gcattgcccg cgcgcatgaa gtaaccgtga agagagtgag
     3901 ccgagcggcc ctcgacggtc aggcaggcgg cccgcaggga ctgcgacaga acctgtccgc
     3961 cgaagaggcg gaactcgccg cggacctcgt tggcacccag aaagagattc tgctcgatgc
     4021 attccaggtc cagcacttcc agcaggcgcg ccgcgcgtcg cgattgatcc gtgtcctgag
     4081 tcatggcgtg cattgtagat tcacgaggga atgacgaata cccacggccg gtaaccggat
     4141 cctgcgggcg ccggccgcgc gcccggcggc ggctcccgac gcggtctcgt ggggcccagg
     4201 cgttgaacct gcccacggat gccagtaagc ttgccgccct gtttgactca ccgtcccgtc
     4261 aggaggccac catgagctgg atgaacagcc gaccgaccgt tgcccgatac ggagacaacg
     4321 cgcgaaagcg gtgagcccgt ccttcgtctg aaatagaccc ccgccggcat ctgccggcct
     4381 gtgatgagga cccgctcacc agcgagcgga tccccctgca gtcatcttcg accccgatcc
     4441 gctccgcgcc tgcccaaccg ggcccaggac ccatgcaacc gagaatctgc gggagcgatt
     4501 gacgatggca gccagacaat cgatcaacct atggcagctg accagaggtc agcggctccg
     4561 ctatgccggg gccatcttcg ccatggggat gaccaacctg ttcatattcg gccccgtgct
     4621 ggtgagcggc tcggcgatcg acgtcatcgc cgatcaggac ctcagccacg ccgtcccgcc
     4681 tctgctctgg gcggccagca ggctttccga gggcgcgccc ttcgccgcct acctctgggt
     4741 ggccgccgtg gtcgcggtca ccatgacggc catcggcggc gccttcctgt tcgcgcgcgg
     4801 ccggtttgca gcggtggcgt cggaggccat cgtccgcaac cttcgcgagc ggctgtacga
     4861 ccggctgcac gatctggaag ccggcttttt cgacacggca gacacgggcg atctcgttca
     4921 gcgcgcgagc tcggacgtgg aaacgctgcg cgtcttcctg gcaagcgacg tggtcgagat
     4981 cggtcgggcc atcatgctca tcatgtgcgt cgcccccatc ctcgcgtggc tggacgtccg
     5041 gcttgccgcg ctgtctctgt gcctgatgcc cctgctggcc atcggagcct acctcttctt
     5101 cgcccgggtg aaagacgtgt tccagataac ggacgagtcc gaagccgcca tgaccgccac
     5161 gctccaggag aacctcaccg gcatccgcgt ggtacgggcg ttcgcgcggc aggcgttcga
     5221 gatcgagaaa ttcggcgcgc ggaacgcaga gttcaggaac aacaatcgcc ggctgatcca
     5281 gctgatgggc atctactgga gcacgagcga tctgttcgcc atgacccaga tcggcatcgt
     5341 gctcattgcg ggggggctgt tcgtgctcga cggttccatc accgtcggca cccttttcga
     5401 gttcatgacc atggtgtcca tggtgatctg gcccgtccgc cagctcggcc gggtgcttac
     5461 ggatacgggc aaggcggtgg tgtcgctggg caggatcaat gaggtgctca cggaagccga
     5521 ggagcgggcc ggcaccatcc cgggcgaggg gcgggcacgc ggggagattc gcatcgaaaa
     5581 cctgacgttc ggctacgacc cgcagcgtcc ggtgctcgag gatgtgaacc tgcacgtgga
     5641 ggtcggcgag acgctggcca tcgtcggccc cccgggatcc ggcaagtcca gcctgatccg
     5701 cgtgcttctg cgcatgtatc cgtaccagaa gggatccgtg cggatcgatg ggtgggaggt
     5761 cgccgagctc gaccggctct ggctgcgcca ccagatcgga gtggtcctgc aggacccctt
     5821 tctctactcg cgcagcattc gcgccaacct tgcgctgggg cggcccggcg cgcccgagaa
     5881 tgagctcatc gaggcatgcc acgacgcggc cgttcacgac gccatcatcg gcttcccaga
     5941 aggtttcgaa gccatggtcg gcgagcgcgg cgtcacgctt tccggcgggc agcggcagcg
     6001 catggccctg gcacgcgccc tgctgaagga cccgcccata ctggttctcg acgattccct
     6061 ttccgcagtg gatacgggaa ccgagcgcag gatcctggat gccatgcgcc gccgcaaggg
     6121 ccgccagacg accctgatca ttgcccaccg actgtcgtcg gtgatgcacg cggaccgcat
     6181 tctcgttctt caggaaggac gtgtggttca gctcggcaat cacgccacgc tggccgggga
     6241 gcccggaccg taccggacgc tctgcgagat ccagggtgcg ctggatgcat ccatcgaggc
     6301 ggacaccaac gccacaggac gcggcaatgg ataactacga cgacgagaac ttcgacgacg
     6361 gcttcgacga cgaagcgctg gcggccaggg tcgacctgaa gctgtggcgc aagctgttcg
     6421 cctacgcccg acgctacccg aaggacctcg tgtggctggc cgtctgcgct ttcaccaccg
     6481 cctcgatgga ggtcacgtat cccctgatta ccaagtgggt ggtagacgac gtcgacgccg
     6541 cttacgcagc cggcacggtg atctccctgt ggcctcatgc gctcgcctac ctcgcgtgca
     6601 cggtgaccat ctgcatcgcc atcggcgggt tcatctggat ggggggaaaa atccgcaccc
     6661 acgtgagcca cgacatacgt cgcgacggct tcgagaacct gcagcggctg tccttcgcct
     6721 tctacgacta ccgtccggtt ggctggctca tggcgcgcat gacgtcggac tgcgaacggc
     6781 tctccaacat cctggcctgg ggattcctcg acctggtctg gggatcaacc atgatgctgg
     6841 gcatcgccgt ggccatgctt gtcatgaacc cgaagctggc cctcgtggtg ctcgctatcc
     6901 tgccgcttct cggctgggtc tcggcccgtt ttcagcgccg gattctgtcc agcgcccgcc
     6961 gcgtacgcgc gacgaattca cgcatcacgg cttccttcaa cgaagccatc atgggcgtgc
     7021 tgaccagcaa ggcgttcgtg cgcgagcagg cgaatctgga cgagttccgg gagctcacca
     7081 ataccatgca cggcgcatcg gtgctgaatc tcagccaggc ggccgtctac gtgcccatcg
     7141 tcatcaccct ggcgagcctc gccacgggtt tggcgctcgc catcggcggg ctggatctgc
     7201 tcggcggcgc catcgccatc ggcaccctgg tcgccttcat ggcctacacg cggcacttct
     7261 tcgaccccat cgagcagctc ggccactggt tcgccgagat gcagatggcc caggcctcgg
     7321 cggagcggat actgagcctc atcgagacag acccggcggt gaaagactca cccgacgtga
     7381 tcgaggctct gcagcgccaa gccgatgcac ccccccgccg gaactacg
//
LOCUS       DBNHKV010000429         7409 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_59217, whole genome shotgun sequence.
ACCESSION   DBNHKV010000429 DBNHKV010000000
VERSION     DBNHKV010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_59217"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..2298
                     /locus_tag="AC16PP_13980"
     CDS             112..2298
                     /locus_tag="AC16PP_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR6482784.1"
                     /translation="MALNADLSHLPTRRRLTNAVTRYVVALGGAAVIGAIALIFFYLL
                     WVAIPIMAGAEIEPGQRLSLPVGTPLLLEANETGEIGLRIDSSGQAIFFSLNDGSIVD
                     RVTLASSLERVKRVLPHSSMYAVQSPQSGIVIYESRHPVTFDNDVRQIRPELNAVFPD
                     TWIDVDPGADFDVHFADFEVLVVAVTGTNLQIQHFRDAEAGFELYEPRRANLSLERVY
                     ERVIIGPRGAWVYLVADDGSVAVLDLQRPTSPRQIFVGKLVAEGSSLNALEPLLGRYS
                     LLAGNDKGQVTQWFMVPGEFGYTLERARTFKLDRAITRLIAEQRRKGFGAVDAEGRFH
                     LLYTTSSRVLADRRLDSNRFSVASIAPRANMLLTGDESGDVTTYRIHNEHPEISWSAL
                     WSKIWYEGYPEPVYSWQSSSADNDFEAKFSLTPLLFGTLKAAFYAMVFAVPIAIMGAI
                     YTAYFMAPAMRRIVKPGVEIMAALPTVILGFLAGLWLAPLIEANLAGVIGSILALPVG
                     ILLTAVLWGRLPEKVTGRFDGWYGIFILPTIILTVVAGFGLGPWIESVFFGGNTRGWF
                     LNELGLAYDQRNALVVGLAMGLAVIPTIFSITEDAIYGVPTHLINGSLALGATPWQTL
                     VRVVILTASPGIFSAVMIGLGRAVGETMIVLMATGNTPVMDMNLFEGMRTFAANIAVE
                     LPESEVGSTHYRILFLTALVLFMITFVFNTAAEVVRQRLRSRYGNL"
     gene            2301..3995
                     /gene="pstA"
                     /locus_tag="AC16PP_13985"
     CDS             2301..3995
                     /gene="pstA"
                     /locus_tag="AC16PP_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR6482785.1"
                     /translation="MIHSDHDSLRAWFRSGELWIWANAAAVGISIIAIVGILILLAAR
                     GFSHFWPADIQLMTYRDDGDRTAVALGEIADSETLSVQQFVESVGGSGRAVPEGLDEV
                     ERLLVKTGNRRYDPPDFTWIFTHKIDAVSFPDDATVFERMEWGNAYGYLRAVGGPQET
                     VSDPALLWDTFGNLMERAAGLRASIEDIERGVLNEINFGMEKVRLERRSIELGGLDVD
                     QAATAMQELDERDLDLEKRYESAEQELRSLYRELQRDYVRVELATGQVIEMRLAEIVR
                     AWRPNQMSALDKLWHYLESFGLFLSEQPREANTEGGIFPAIFGTVLMVLLMSVLVTPF
                     GVIAAVYLREYARQGAFVRLIRIAVNNLAGVPSIVYGVFGLGFFVYFLGGNLDQLFFP
                     EALPAPTFGTPGLMWASLTLALLTVPVVVVSTEEGLTRIPRAIREGSLALGATKAETL
                     FKVVLPMASPSILTGVILAVARAAGEVAPLMLVGVVKLAPTLPLDGNFPYLHLERKFM
                     HLGFHIYDVGFQSPNVEAARPLVYATALLLVLIIIILNLAAIMLRSRLEDRYRGVE"
     gene            4077..4865
                     /gene="pstB"
                     /locus_tag="AC16PP_13990"
     CDS             4077..4865
                     /gene="pstB"
                     /locus_tag="AC16PP_13990"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR6482786.1"
                     /translation="MTDTKQNASGSNGTSLLVDRLSLFYSGQKALHEISLEIPRKQAT
                     AFIGPSGCGKSTLLRCFNRMNDLIDGVETTGRILLDGTDINSKEVDVAQLRRLVGMVF
                     QRPNPFPKTIYENVAYGLRLQDTLPKTEMDERVEWALRSAALWDEAKDRLKESALSLS
                     GGQQQRLVIARAIAIKPSVLLLDEPASALDPISTLKIEELIHQLKTEYTIVIVTHNMQ
                     QAARVSDYTAFLYLGELIEFGKTDTLFTNPKERQTEDYITGRYG"
     gene            4900..5607
                     /gene="phoU"
                     /locus_tag="AC16PP_13995"
     CDS             4900..5607
                     /gene="phoU"
                     /locus_tag="AC16PP_13995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR6482787.1"
                     /translation="MTPHPHISEQFDHELDQARNRLMEMGGKVEQQLKNACKALTNHD
                     LELAETIKTGDRSINQLEVDLDELCVQIIARRQPAASDLRTLVSVMKASTDLERIGDE
                     AKRIAKMAIAISRLTYPRDQYTDIRKLVALVMAQVSATLDAFARLDVDKAIGLIRYDA
                     EIDDAYDAIVRDRAVQMKEMANNPEGIEQTLHVLWAARALERIGDHAKNISEVVVFLV
                     RGEDIRDRALAAKLTGG"
     gene            complement(5617..6402)
                     /locus_tag="AC16PP_14000"
     CDS             complement(5617..6402)
                     /locus_tag="AC16PP_14000"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6482788.1"
                     /translation="MDLQEGVAIVTGSSSGVGAATARQLAERGAHVVINYASNEDGAR
                     ATQEACEAFGVETAVVRANVADDADCRRLAATAEERWGRIDALVNNAGTTKFCAHHDL
                     EGLDSDDFMNIYGVNLVGPYQMTRAVAPAMKRGGRGSIVNVASIAGVTGIGSSIAYAA
                     SKGALVTMGLSLARVLGPEIRVNTVCPGFIQGDWLEQGMGAEIYARTKKFLEESTPLR
                     LTTTPDTIAEAIVYFLSGADVVTGETLILDGGHHLNQMPFARR"
     gene            6522..7172
                     /locus_tag="AC16PP_14005"
     CDS             6522..7172
                     /locus_tag="AC16PP_14005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR6482789.1"
                     /translation="MDQMTISAILWDFGGVLTTSPFEAFNRFEAEHGIPADFIRRINA
                     TNPDGNAWAQFESSAIGLNEFDEAFARESAAQGHRIPGRKVLELLSGELRPRMVEALH
                     SCKVSYQVACITNNVKSGQGPAMASNRARAAAMEGVMALFDLVVESSIEGIRKPDPRI
                     YELTCERLGVRPEQAVFLDDLGVNLKPARALGMHTIKVVEESQALEELGSLTGLAL"
     gene            complement(7182..>7409)
                     /locus_tag="AC16PP_14010"
     CDS             complement(7182..>7409)
                     /locus_tag="AC16PP_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin family protein"
                     /protein_id="HIR6482790.1"
                     /translation="FPEHLLQHTGGEREIEVPAADFRELLINLERRWPEAGAVLEKCA
                     VAIDGQIYQDAFLEPLEPDSEVFFMQRIEGG"
BASE COUNT         1445 a         2386 c         2186 g         1392 t
ORIGIN      
        1 ggtcaggaaa tcgttgccaa agacggctat attccggtct cggcgagcgt cgtcagccgc
       61 gagctcagaa aacttcaata agcgcccacc gagcgaatcg aagaacccgc aatggccttg
      121 aacgccgatc tgtcgcacct gcctacccgc aggcgtctga ccaatgccgt cacgagatac
      181 gtggtggccc ttggcggcgc ggcggtcatc ggcgccattg cgctgatctt cttttacctg
      241 ttgtgggtgg ccatcccgat catggcaggc gcggagatcg agcctggaca gcggctgtct
      301 ctccccgtcg gcacgccgct actgctcgag gccaacgaga cgggcgagat cggtctgcgc
      361 atcgactcgt cgggacaggc gatcttcttt tcgctcaacg atggcagcat cgtcgaccgg
      421 gtcaccctgg ccagttccct ggaacgggtg aaaagggtcc tgccccactc ctccatgtac
      481 gccgtgcaga gcccgcagag cggtatcgtc atctacgaat ccagacatcc ggtgacgttc
      541 gacaatgacg tgaggcagat ccggcccgag ctcaacgcgg tatttccgga tacctggatc
      601 gacgtcgacc ccggcgcaga tttcgacgtc cacttcgccg acttcgaagt tctcgtggtc
      661 gccgtgaccg gaacgaacct tcagattcag cactttcggg acgcggaggc gggtttcgag
      721 ctctacgagc cccgacgggc caacctctcc ctcgagcggg tctacgaacg cgtcatcatc
      781 gggccgcgcg gcgcgtgggt ttatctggtc gccgatgacg gatcggtcgc cgtgctcgat
      841 ctccaacgcc caacctcgcc gcggcagatc ttcgtgggca agctggtcgc cgaaggctcg
      901 tcgctgaatg cgctcgaacc cctgctcgga cgctattctc tcctggcggg gaacgacaag
      961 ggccaggtga cccagtggtt catggttccc ggcgagttcg gttacaccct ggagcgcgct
     1021 cgaaccttca aactcgaccg cgcgatcacg aggctgatag ccgagcagcg ccgcaaaggc
     1081 ttcggagcgg tggacgccga ggggcgcttc cacctccttt acacaacctc atctcgcgtg
     1141 ctggcggatc gtcggctcga ctcgaaccgc ttctccgtcg cctccatcgc gccccgtgcg
     1201 aatatgctgc tcaccggtga cgaaagcggc gacgtcacga cctaccggat tcacaacgaa
     1261 cacccggaga tctcctggag cgcgctgtgg tcgaagatct ggtacgaggg atatccggaa
     1321 cccgtttaca gctggcaatc atcatcggcc gacaatgact tcgaggcgaa gttctccctg
     1381 acgccgctgc ttttcgggac gctgaaggcg gccttttacg ccatggtgtt cgccgtgccg
     1441 atcgccatca tgggggcgat ctacaccgca tacttcatgg cacctgccat gcgccgtatc
     1501 gtcaaacccg gcgtagagat catggccgcg ttacccaccg tgatcctcgg atttctggcc
     1561 ggcctatggc ttgcgccact catcgaggcc aacctcgccg gcgtcatcgg cagcatcctg
     1621 gcgctgccgg tggggatcct gctgacagcc gtcctctggg gccgtctgcc agagaaggtg
     1681 accggacggt ttgatggctg gtacggaatt ttcatcctgc caacgatcat cctcaccgtg
     1741 gtcgcgggct ttggcctcgg cccgtggatc gagagcgtct ttttcggcgg caacaccaga
     1801 ggctggtttc tgaacgagct gggcctcgcc tacgaccagc gcaatgccct ggtcgtcggc
     1861 ctggcgatgg gactcgccgt gatacccacg attttctcga ttaccgaaga tgccatttac
     1921 ggtgtgccca cccacctgat caacggatca ctcgctctgg gagccacccc atggcagacc
     1981 ctggtccgcg tggtcatcct cactgcgagt ccggggattt tctccgcggt gatgatcggg
     2041 ctcggccgcg ccgtcggcga aaccatgatc gttctcatgg ccaccggaaa cacgccggtt
     2101 atggacatga acctcttcga aggtatgcgg acttttgcag caaacatcgc cgtggagctg
     2161 ccggaatcgg aggtcggcag cactcactat cggatcctct tcctaaccgc gctggtcctg
     2221 ttcatgatca ccttcgtgtt caacacggct gccgaggtcg tccgtcagcg tctgcgatca
     2281 cgctacggga acctgtgacg atgattcact cggaccacga cagccttcgc gcctggttcc
     2341 gttcaggcga gctatggatc tgggcgaatg ccgcagcggt tggtatcagc atcattgcca
     2401 tcgtcggcat actgatactc ctggcggcac ggggcttctc gcacttctgg cccgccgaca
     2461 ttcagctgat gacctatcgg gacgacggcg accgcacggc ggtggcactg ggggagatcg
     2521 cggactccga aaccctgtcc gttcagcagt tcgtagagtc cgtgggcgga tccggacgag
     2581 ccgtccccga aggtctcgac gaggtagagc ggctcctcgt gaagaccggc aaccgtcgat
     2641 acgaccctcc ggacttcacc tggatcttca cccacaagat cgacgccgtc agctttccgg
     2701 atgacgccac cgtcttcgag cgcatggagt ggggaaacgc atacggatat ctgagagccg
     2761 tcggcggccc acaggagacc gtctcggatc cggcgctgct ctgggacacg ttcggcaacc
     2821 tgatggagcg cgccgctggt ctgcgcgcat ccatcgagga catcgagcgg ggcgtgctca
     2881 acgagatcaa tttcggcatg gagaaggttc gcctggaacg ccggagtatc gagctgggcg
     2941 gcctggacgt cgaccaagcg gccaccgcca tgcaggaact cgacgagcgc gatcttgatt
     3001 tagagaagcg ttacgagagc gcagagcagg aactacgttc gctctaccgg gaactccagc
     3061 gcgattacgt ccgggttgag ctggcaaccg gacaggtcat cgagatgagg ctggccgaga
     3121 tcgtccgcgc ctggcggccg aaccagatgt ctgcgctgga caaactttgg cactacctgg
     3181 aaagcttcgg cctctttcta tctgagcagc ctcgcgaagc caacaccgag ggcggcatct
     3241 ttcccgccat tttcggtacc gttctgatgg tgctgctcat gtccgtcctg gtcacgcctt
     3301 tcggcgtcat cgcggccgtc tacctcagag agtacgcccg gcagggcgca ttcgtccggc
     3361 tcatccggat tgccgtcaac aaccttgccg gggtgccttc gatcgtttac ggcgtgttcg
     3421 gcctgggatt cttcgtctac tttctcggcg gcaacctcga ccagctgttc tttcccgaag
     3481 cgctcccggc gcccaccttc ggaacaccgg gcctgatgtg ggcttcgctc acgctcgccc
     3541 tgctgaccgt accggtggtc gtcgtatcca ccgaggaggg gttgaccaga atcccgcgcg
     3601 ccatccgtga aggcagcctg gccctgggcg ctaccaaagc cgagacgctg ttcaaggtcg
     3661 tcctgcccat ggcgagcccg tcgatcctga ccggcgtcat cctggcggtg gcacgagccg
     3721 ccggcgaagt tgctccgttg atgctcgtcg gggtcgtgaa gctggcgccc acgctgccgc
     3781 ttgacgggaa ctttccctac ctgcatctgg agcgaaagtt catgcacctc ggcttccaca
     3841 tctacgacgt tggtttccag agccccaacg tggaagccgc ccgcccgctg gtctacgcga
     3901 ccgccctgct gctggtgctg atcatcatca ttctgaacct cgccgccatc atgctgcgca
     3961 gccgtctgga agaccggtac cggggggtgg aatgaccgtc aaacgaaaag aacgagcctc
     4021 ttctaagcag cgcgagcgcg ctgatcaccg cctcctttac ggatgaaccc ctagagatga
     4081 ccgacacgaa acagaacgcg tcaggatcga acggcacgag cctgctggtg gaccgtcttt
     4141 cccttttcta cagcgggcag aaagcattgc acgaaatctc cctcgagatt ccgcgcaagc
     4201 aggcaacggc tttcatcggc ccctcgggtt gcggcaagtc cacgctgctg cgttgcttca
     4261 accggatgaa cgatctgatc gacggcgtgg aaaccaccgg caggatcctg ctcgacggca
     4321 ccgacatcaa ttccaaggaa gtcgatgttg cccagctaag aaggctggtc ggcatggtct
     4381 tccagcgtcc gaacccgttt ccgaaaacca tctacgagaa cgtcgcctac ggcctgcggc
     4441 ttcaggacac gctgccgaaa accgaaatgg acgagcgcgt cgaatgggcc ctgcgaagcg
     4501 ccgcgttgtg ggacgaggcg aaggatcgtt tgaaagagtc tgcgctcagc ctgtccggcg
     4561 ggcagcagca acgactcgtc atagcacgcg ccattgccat caagccgtcg gtgttgctcc
     4621 tcgacgaacc tgcctccgcc ctggatccca tttcaacgct caaaattgag gaactgatac
     4681 atcaactgaa aaccgagtac actatagtaa tcgtaactca caacatgcag caggcagcca
     4741 gggtatccga ctacaccgcg tttctttacc tgggggaact gatcgaattc ggcaagacgg
     4801 acactctttt cacaaatccc aaagagcgcc agaccgaaga ctacataacg gggcgctacg
     4861 gctagacaga aacgcgaaag gacgcgacag gaatacgaaa tgacccccca tcctcacata
     4921 tccgaacagt tcgatcacga actcgaccag gcacgcaacc gtctcatgga gatgggaggc
     4981 aaggtcgaac agcagctgaa gaacgcctgc aaagccttga ccaatcacga cctggaactc
     5041 gccgagacga tcaagaccgg cgatcgcagc atcaaccagc tggaagtcga tctggacgag
     5101 ctctgcgtgc agataatcgc ccgccgccaa cctgctgcat cagacctgcg caccctcgtc
     5161 agcgtgatga aagccagcac cgatctcgaa cgtattggcg acgaggcgaa acggatcgcc
     5221 aagatggcca tcgcgatatc acgtctgacc tatccgcggg atcagtacac ggacatccga
     5281 aagctcgtcg cgttggtgat ggcccaggtt tcagcaacgc tggatgcgtt tgcccgactg
     5341 gacgtcgaca aggcaatcgg cttgatccgg tacgacgcag agatcgatga cgcctatgac
     5401 gccatcgtcc gggaccgagc ggtacagatg aaggaaatgg ccaataaccc ggagggcata
     5461 gaacagaccc tccacgttct ctgggccgcc agggctctgg aacggatcgg cgatcatgcc
     5521 aagaacatca gcgaggtcgt ggtgttcctc gtcagaggcg aagatatccg cgaccgcgca
     5581 ctggcggcca agctgaccgg cggctgagtt accttctcag cgacgcgcga agggcatctg
     5641 attcaaatgg tgcccgccat ccagtatgag cgtctctccc gtgaccacgt ctgcaccgct
     5701 cagaaaataa acgatggcct cggcaatggt gtccggcgtg gtggtcaggc gcagaggcgt
     5761 ggactcctcg aggaacttct tggtccttgc gtagatctcc gcgcccatgc cctgctccag
     5821 ccaatcgccc tgaatgaacc cgggacagac cgtgttcacg cgaatctcgg ggccgaggac
     5881 tctggcgagc gacaggccca tggtaacgag cgctcccttg gaggccgcgt aggcgatgga
     5941 gcttccgatt ccagtcacgc cggcgatgga ggcgacgttg acgatgctgc ctctgccccc
     6001 ccgtttcatg gccggcgcga ccgcccgcgt catctggtag ggacccacca ggttcacgcc
     6061 gtaaatgttc atgaagtcgt ccgaatcgag gccttcgaga tcatgatgag cacagaactt
     6121 ggtcgtcccc gcattgttga ccaacgcgtc tatcctgccc caccgctctt cggccgtcgc
     6181 agcaaggcgc cgacaatcgg cgtcgtcggc cacgttggcg cggacgacgg cagtttccac
     6241 cccgaacgct tcgcaggctt cctgggttgc tctggcgcca tcctcgttgc tcgcatagtt
     6301 gatgaccacg tgggcgcccc gctcggccag ctgacgagct gtggccgcac ccacacccga
     6361 agacgatccg gtgacgattg ccacaccttc ctgaaggtcc atccgcctgc tcccactctg
     6421 cgcgaccggg agaacctagc agactcctcc tgcgcccacc agccgttgcg gtggaggctc
     6481 cccggtcatt gatttagaat ccgccgcctc ctgagcaacc catggaccaa atgaccatca
     6541 gcgcgattct ctgggacttc ggcggggttc tgaccacaag cccattcgag gccttcaatc
     6601 ggttcgaagc ggagcacggg atccccgccg acttcatccg ccgcatcaat gccacgaacc
     6661 cggatggcaa cgcatgggcc cagttcgaat ccagcgccat cgggctgaac gagttcgacg
     6721 aggcgtttgc ccgggagtcc gccgcgcagg ggcaccgcat tcccggaaga aaggtcttgg
     6781 agttgctctc gggcgagctg cgtcccagga tggtcgaagc cctgcacagc tgcaaggtgt
     6841 cgtatcaggt ggcgtgcatc accaacaacg tcaagtccgg ccagggaccg gccatggcaa
     6901 gcaaccgggc gcgagccgcg gccatggaag gggtcatggc gctgttcgac ctcgtggtgg
     6961 agtccagcat cgaggggatc cgtaagcccg acccgcggat ttatgagctc acgtgcgagc
     7021 ggctcggcgt ccgacccgag caggccgtgt tcctcgacga cctcggcgtc aacctcaaac
     7081 ccgcgcgtgc cctgggcatg cacaccatca aagtggtcga ggaaagtcag gcgcttgaag
     7141 aactcggctc cctcactggg cttgcgctct gagagggacg ctcagccgcc ctcgattcgc
     7201 tgcatgaaga agacctcgct gtctggctcg aggggttcca gaaaagcgtc ctgatagatc
     7261 tggccatcga tggccaccgc acacttctcc agcaccgccc ccgcctcggg ccaacgcctt
     7321 tcgaggttga tcaggagctc gcgaaaatcg gccgcgggca cctcgatctc ccgctctccg
     7381 cccgtgtgct gaagcaggtg ctcgggaaa
//
LOCUS       DBNHKV010000430         5360 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_983586, whole genome shotgun sequence.
ACCESSION   DBNHKV010000430 DBNHKV010000000
VERSION     DBNHKV010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_983586"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..62)
                     /gene="ffs"
                     /locus_tag="AC16PP_14015"
     ncRNA           complement(<1..62)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC16PP_14015"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            298..984
                     /locus_tag="AC16PP_14020"
     CDS             298..984
                     /locus_tag="AC16PP_14020"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR6482791.1"
                     /translation="MHRSIERAYVNHFVAPQLERMGSGFKIMKPWNLRVHGPNIGFGK
                     CVHVITARDRKVMLTVWEHDAGAGRIDIGDYCLICPGVRLDSACGIEVGDNTMIASSA
                     YITDADWHDVYDRSRPIGASAPVVLGENVWIGDSAIVCKGVRVGANSIVGAGAVVTHD
                     VPANTIAAGNPARVVKELDPSRTLVRRADLLDDPDGLDLRMDDLDRYLLGNNTWLGWL
                     RSRIAPRRSD"
     gene            complement(993..1811)
                     /gene="queF"
                     /locus_tag="AC16PP_14025"
     CDS             complement(993..1811)
                     /gene="queF"
                     /locus_tag="AC16PP_14025"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03138.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HIR6482792.1"
                     /translation="MSRTNPLGRETGIPEGYSPGVLYPMSRVDHRDALGIRAALPFTG
                     EDVWSAFEFSWLNARGKPEVAMVRVQVPCDSPCIVESKSMKLYLGSFSQTRVDSTTRV
                     QGMLAADLASAFGTEVEVGLVSLADMSAIGEGPPGLCLDELDVDIHSYDYAVETLAVL
                     PGGEEVHRETLHSNLFRSICPVTGQPDWATIIVRYSGPAMDRAALLGYLIGFRRHPAF
                     HEAVVEQIFMDLSQRCGAKELSVHGCFLRRGGIDISPFRSNFEGPPPPMRLLRQ"
     gene            2169..3272
                     /locus_tag="AC16PP_14030"
     CDS             2169..3272
                     /locus_tag="AC16PP_14030"
                     /inference="COORDINATES: protein motif:HMM:NF023828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HIR6482793.1"
                     /translation="MTDIATTSCSNCGATKSGEFCAICGQNSRNYLRAAYRIVGDLLS
                     ETFEVDSRLFISLRHLILKPGFLSNEFSAGRRARYVSPARMYLVVSLIFFFTLSVTQR
                     DSIGLDAEGNPVDLSESLFSMEDVDSKEGLRAALEAAGESRRAELIDVLTEHGVDASR
                     LEAELERLEAELAESIETTEQKAGETNESEMTDPAQEAPASRELKINLDLGEEDSELE
                     ARLEQKFKDMASRPADALEDFLENLPVMMFILLPFAAMLLKLAYPRRFYSEHFVFALH
                     LHSFYYLLLTVMLFLPEQADASTGSEGSAVWEWVSNGLVMISVLYAFIALKSTYRQSI
                     GRTLAKGVGLAFGYSILLGIGMLATGVVTFIYY"
     gene            complement(3319..3954)
                     /locus_tag="AC16PP_14035"
     CDS             complement(3319..3954)
                     /locus_tag="AC16PP_14035"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: protein motif:HMM:NF016382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003727 - single-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HIR6482794.1"
                     /translation="MQNRLRDRVRIVPGPKPRRIVGLDCAFAENDVLAVAVVWDVVGG
                     RVLETRGARRRVDFPYVPGLLSFREVPVMLDVLRRLTTSFDAVMCDGQGIAHPRRLGL
                     ASHLGVILQMPTLGCAKSRLLGSHAPVGRRRGDWVPLLDGDERVGTVLRTRDDVRPVF
                     VSPGNLCNHDDAVGWVLACGGGYRLPEPTRLADRHVAAYRRQGSYRGGLTS"
     gene            4137..>5360
                     /locus_tag="AC16PP_14040"
     CDS             4137..>5360
                     /locus_tag="AC16PP_14040"
                     /inference="COORDINATES: protein motif:HMM:NF025761.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HIR6482795.1"
                     /translation="MKQEPLTEPYQRRQLADLPPSGVAYDEVLDGRELRHHWQFLDGT
                     FTATDSRSPEALNRTVQQILDESAVTYLEDGAQRPWRLDGLPYVLDPNEWPTLTRGLE
                     QRARLLNAVVADLYGPQTLLHRGLPLGLAFANPDYLLPACGYSAPEAVFLHLLAFDLG
                     RSPDGQWRVLSSRSEAPSGLGYTLENRLIMARSVPELFESGGIARIAQFFRAHSEYLH
                     ELGETTGRSDGLSVILSGGPGQESYFEHTFLGRYLGYPVVEGADLTVRDGEVFLKTLE
                     GLKPVSLIVRQVESSDCDPLELRAHSLQGVPGLLRAASTGRVAVANAIGSGLVENEAF
                     ASFLPSLCKQMLSEPLELPDLTTWWCGQPAELEHVLANRGELVLRNAFHRKPLLASSV
                     GAYMASDILPAGNASI"
BASE COUNT         1037 a         1747 c         1648 g          928 t
ORIGIN      
        1 ctaccgttgc tcccttccgg gcctggcggg gttcacaacc tgtcgttgcg gggggaccgg
       61 aatgggtcac cattgcgcgg ccgccagcgg ccacggcgaa agtatgctag cggagatggg
      121 taacgaagtg tagcggcggc attccgcagt tgccgcaccg ccgcggatgc ccgacgctcc
      181 cccgccgctc gccagcctcc gcctggcccg ccggacgacc atgggctaat ctgccccgca
      241 ttcccagagg gagacacagg ggtgcgaagg gatcaccgac cgtacgcagc caagcggatg
      301 catcgatcca tcgaacgcgc ctacgtcaat cacttcgtag cgccgcagct cgagcggatg
      361 gggtccggtt tcaaaatcat gaaaccatgg aacctgagag tccacggacc caacatcggg
      421 ttcgggaagt gcgtccacgt catcaccgcc cgggaccgca aagtcatgct cacggtgtgg
      481 gagcacgatg ccggcgcggg gcgtatcgac atcggcgact attgcctcat ctgccccggc
      541 gtgcgcctgg attccgcctg cgggatcgag gtgggcgaca acacgatgat cgcctcgagt
      601 gcctacatca ccgacgccga ctggcacgac gtctacgacc gctcacggcc gatcggtgca
      661 agcgcgccgg ttgtgctggg cgagaacgtg tggatcggcg acagcgccat cgtctgcaag
      721 ggcgtcagag tcggcgccaa cagcattgtc ggcgccggcg cggtcgtgac ccacgacgtg
      781 ccggcaaaca ccatagccgc cggaaacccg gcgcgcgtgg tcaaggagct ggatccctcg
      841 cgcaccctgg ttcgccgggc ggacctgctt gacgatccgg acgggctcga cctgcgcatg
      901 gacgatctcg accgctacct gctcggcaac aacacgtggc tcggttggct gagatcccgg
      961 atcgcgcctc gtcggagcga ttgatcccgc ttctactgac gcagcagccg catcggtggc
     1021 gggggcccct cgaaattgga acggaagggg ctgatatcga taccgccacg gcgcagaaag
     1081 cacccatgga cggaaagttc cttcgcaccg caacgctgcg aaagatccat gaagatctgc
     1141 tcgaccacgg cttcgtgaaa cgccgggtgt cggcgaaagc caatcaggta gccaagcagc
     1201 gccgcccgat ccatggccgg cccggagtaa cgcacgatga tggtcgccca atccggctga
     1261 ccggtcaccg ggcagatgct gcggaacagg ttcgagtgca gcgtctcccg atgaacctct
     1321 tcgccccccg gaagcacggc cagagtctcg accgcgtaat cgtagctatg gatgtcgacg
     1381 tcgagttcat ccagacacaa gccgggcggt ccctcgccga tcgcgctcat gtcagccagg
     1441 gaaaccagac cgacttccac ctcggttcca aaggcggaag ccagatcggc ggccagcatc
     1501 ccctgcaccc gcgtggtact gtcgacccgg gtctgagaaa aagagccgag gtatagcttc
     1561 atggatttgc tctcgacgat gcagggcgag tcgcagggga cctgaacccg gaccatcgca
     1621 acctccggtt ttccccgggc attcagccag gagaactcga aggcggacca gacgtcttcc
     1681 cctgtgaacg gcagcgcggc gcggatcccc agagcgtccc ggtgatccac gcgggacatc
     1741 gggtagagaa cccctggcga atagccttca ggaatgcccg tttcgcggcc tagcgggttg
     1801 gttcgtgaca cttccgactc gttcgactct tcacagacgt acacgtttgc gcaagccggc
     1861 gggtccgtca acacgcttcc atcaacggcc cgccgcatag tgccgcggaa aagaacatgg
     1921 cttgaccttc cacccacgac gcccgaggaa tatctcaata aaaagcgttt accttcatat
     1981 acttatatat attttataca tttaattata ggtatatcgg atactcgcgc tgctaccggg
     2041 cggcaggcgt atcttctggt gagcaaccac gaggtgccgc cgcggcaggc gcctgtctct
     2101 ggtgcatttt ctggatgatc cgcgcgctgc tgcggcatac ttccaaggtc tgcggatcgg
     2161 atgacttcgt gaccgacata gcaaccacat cctgctcgaa ctgcggagcc accaaatcgg
     2221 gagagttctg cgcgatctgc ggtcagaaca gccggaacta cctgcgcgcc gcctaccgaa
     2281 tcgtcggcga tctgcttagc gagacgttcg aggtcgattc caggcttttt atcagcctca
     2341 gacatctgat tctaaaaccc gggtttctca gcaacgagtt ctccgccggg cgccgggcgc
     2401 gatatgtctc tcccgcgcgc atgtacctgg tggtcagcct gattttcttc ttcacgctct
     2461 cggtgaccca gcgcgactcg atcggtttgg atgctgaagg caatcccgtg gacctgtcgg
     2521 aatctctctt ctcgatggag gacgtcgaca gcaaagaagg tcttcgtgct gctttagagg
     2581 ccgcggggga atcccgacgg gcagagctga ttgacgtgct gaccgagcac ggcgtggacg
     2641 cctcgaggct ggaggcagag ctcgaaagat tggaggcgga actcgccgag tcgatcgaga
     2701 cgacagagca gaaggctggt gaaacgaacg agtcggaaat gaccgacccg gctcaagagg
     2761 cccctgcaag ccgagagctg aagataaacc tcgatctggg agaagaagat agcgagctgg
     2821 aggcgcgtct ggagcagaaa ttcaaagaca tggcatcgcg ccccgcggac gctctggagg
     2881 acttcctgga gaatcttccc gtcatgatgt tcatcctact gccgttcgct gccatgctgc
     2941 tgaagctcgc ctacccgaga cgcttctact cggagcattt cgtgttcgcg ctgcacctgc
     3001 actctttcta ctacctgctg ctgaccgtta tgctcttcct gcccgagcaa gccgacgcga
     3061 gcacggggag cgagggcagc gccgtctggg aatgggtgtc caacggcctc gtgatgatca
     3121 gcgtcctcta tgccttcata gcgctgaaga gcacctaccg acagtccatc ggcaggaccc
     3181 tcgccaaggg agtcggcctg gcattcggtt attcgatcct gctcggaatc ggcatgctgg
     3241 caaccggagt cgtcaccttc atctactatt gatcccgccg acggcgacgg catcgacacg
     3301 atccgctgca gccatgacct agctcgtcaa accgccccga tacgaccctt gccttctgta
     3361 ggctgcaacg tggcgatctg ccagacgggt cggctcgggg aggcgatagc ctcccccgca
     3421 ggcgagcacc cagcccacgg catcgtcgtg gttgcacaga tttccaggac tgacaaacac
     3481 gggccgcacg tcgtcgcgcg tgcgaaggac cgttcccaca cgctcatcgc catccagcag
     3541 cggcacccaa tcgccgcggc gccggccaac gggagcgtgg ctacccaaca gccgggattt
     3601 agcgcagcca agcgtcggca tttggagaat gacgcccagg tgcgatgcaa gtcccaggcg
     3661 ccgaggatga gcaatgccct gaccgtcgca catcacggcg tcgaaggaag tcgtcaggcg
     3721 gcgcagcacg tcgagcatca ccggaacttc gcgaaaggaa aggagccccg gaacgtaagg
     3781 aaagtcgacc cggcggcgcg cgccacgcgt ctccaagacg cgccctccga cgacatccca
     3841 gaccaccgct accgcgagaa cgtcgttttc cgcgaaagcg caatccagcc cgacgattct
     3901 ccgcggtttc ggtcccggga cgattcggac ccgatcccgc agacgattct gcacggccac
     3961 cgcggcgcga ggcgcgagac gccatctgtg cggggcccga ggaacggaat tggggcgatc
     4021 gtgccgaggc attacggctt gcaggggcca agccccccac ctaaccttgt ccggcccccc
     4081 actatgcacg gcgggatccg gttcgcaaag ccgacctttc tgagcgacag gccaacttga
     4141 agcaggaacc tctcacagaa ccttaccaga gaaggcaact ggccgacctg ccaccctcgg
     4201 gcgttgccta cgacgaggtg ctggatggca gggagctgcg ccatcactgg caatttctcg
     4261 acggcacgtt cacggcgacg gacagccgct cgccggaagc cctcaaccgg acggttcagc
     4321 agatcctcga cgaaagcgcg gtgacctacc tggaagacgg cgctcagcgc ccctggcgtc
     4381 tcgacggcct gccatacgtc ctggatccca acgagtggcc cacgctaacc cgcggcctgg
     4441 aacagcgggc gcgtctgctt aacgccgtcg tcgccgatct gtacggtccg cagacactgc
     4501 tgcaccgcgg cctgcccctc ggtcttgctt ttgccaaccc cgactacctg ctgccggcgt
     4561 gcggctactc cgcccccgag gccgtctttc tgcatctgct cgcattcgat ctgggacgat
     4621 cacccgatgg gcagtggcgg gtgctgagca gccgcagcga agccccttcc ggtctgggct
     4681 acacgctgga gaatcggctg atcatggcgc gatcggtgcc cgagctgttc gagtcgggcg
     4741 gcatcgcgcg aatcgcgcag ttcttccgcg cccacagcga gtacctgcac gagctgggag
     4801 agacgaccgg acgcagcgac ggtctttccg tcatcctctc cggaggtccc ggccaggaaa
     4861 gctacttcga gcacaccttc ctcggccgct accttggcta tcccgtggtg gagggagccg
     4921 atctgaccgt ccgcgacggc gaggtcttcc tgaaaactct cgaagggctg aagccggtca
     4981 gcctgatcgt ccggcaggtg gagtcgtccg actgcgaccc gctggagctg cgcgcgcatt
     5041 ccctgcaggg ggtgccaggc ctgcttcgtg ctgcgagcac ggggcgggtg gcggtggcaa
     5101 acgccatcgg cagcggcctg gtggaaaacg aagcgttcgc gagtttcctg ccctcgctgt
     5161 gcaagcagat gctgagcgag cccctcgagc tgccggatct caccacctgg tggtgcggcc
     5221 agccggccga gctggaacac gtccttgcca accgggggga gctggtgctg cgtaatgctt
     5281 tccaccgcaa gccgctgctg gccagcagcg tgggcgcata tatggccagc gacattctcc
     5341 cggctggcaa cgcatcgatt
//
LOCUS       DBNHKV010000431         8843 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1479461, whole genome shotgun sequence.
ACCESSION   DBNHKV010000431 DBNHKV010000000
VERSION     DBNHKV010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8843
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1479461"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="AC16PP_14045"
     CDS             <1..477
                     /locus_tag="AC16PP_14045"
                     /inference="COORDINATES: protein motif:HMM:NF013626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HIR6482796.1"
                     /translation="KIQAARIAARSGAHTVITSGRIEGVLNRVLEGADVGTLLLASMN
                     PLDARKRWIAGQLKVKGELELDRGAVEALRRRGVSLLPVGVSAVRGRFVRGDVVRCVD
                     GDGLAIAQGLVNYSSEEAARLLRAGSDEIADRLGYAAEPELVHRDNLVLLSGGSKP"
     gene            complement(478..744)
                     /gene="rpsT"
                     /locus_tag="AC16PP_14050"
     CDS             complement(478..744)
                     /gene="rpsT"
                     /locus_tag="AC16PP_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011336008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR6482797.1"
                     /translation="MANSPQARKRARQAEKRRRQNASQRSMVRTYIKRVDAAIQSREY
                     DAASAALNDAIPVIDRMASKGIMHKNKAARHKSRLNAKVAALKA"
     gene            804..2387
                     /gene="murJ"
                     /locus_tag="AC16PP_14055"
     CDS             804..2387
                     /gene="murJ"
                     /locus_tag="AC16PP_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR6482798.1"
                     /translation="MSTGTEKPEQQAAALEGPQNVAAQGGIVSGMTLLSRITGLVRDM
                     ALSYFFGAQDVADAFFVAFRIPNFFRRLFAEGAFSQAFVPVLARYRGGGVGELRRFVA
                     VMQGNLIAALSVFVLLGVIFSPALVAVFAPGFFGDDERFALTSSMVRITFPYLGLISL
                     TAFAGALLNSHHRYAVPAFTPVLLNLSLIAAVLFAADVFATPVFALAWGVLVAGFAQL
                     AFQGPSLRRLGLLVAPRISRTDPGARQVGKLLIPAVLAASVNQINALIDTMLASTLVT
                     GSISWLYYSDRLLELPIGLVAVALGTVLLTTLSRLDSSGDRAGFAATVDWGMRMGAIL
                     GVPAAAALYVLAVPLVASIFLRGALTPLDARMASLSLQAFAVGLPALVLVKVLAPAYF
                     ARRDTATPFRIGAVAVGVNVALNLALFFWMGHVGLALATSASAWVNALMLLRGLVSGD
                     AYRPGRPFLVSTARALAASAVMVAAFAYWVPEPSAWMAAPDGERVVWLAGVVAGGGSV
                     YLLCLIVFGERPRNLLHRA"
     gene            2408..3334
                     /gene="ribF"
                     /locus_tag="AC16PP_14060"
     CDS             2408..3334
                     /gene="ribF"
                     /locus_tag="AC16PP_14060"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR6482799.1"
                     /translation="MEIIRGLHNVKPRHRGCVLTIGNFDGVHHGHQVLLAHLIAKSEE
                     LGVPSMLMTLEPAPREFFAGETVPARLTRFREKVTLLEPTGLHRILCLPFNERIASIP
                     AEWIVEELLHNQLGIRYIVVGDDFRFGKGAAGDYRMLQAAGERYGFEVSHIGTLTFDH
                     ERVSSTRIRECLSAGDFALAEKLLGHPYFIMGRVVYGRQLGRKLGIPTANIQLQRYRA
                     ALEGVFAVSVRGLEKEYQGVANVGIRPTVDGREPLLEVHIFDFDRDIYGTLLTVEFRH
                     KIRDEIAFDGLDALKAQIQEDQRTARAWFANE"
     gene            3354..3851
                     /gene="lspA"
                     /locus_tag="AC16PP_14065"
     CDS             3354..3851
                     /gene="lspA"
                     /locus_tag="AC16PP_14065"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR6482800.1"
                     /translation="MLKWLGLSAIVVVLDQATKWWIVQEFRLGERMDWLPFLSWVRWH
                     NDGAAFSLLSGAGGRWFFVALAVAFAVFIIWELRRLPVGGRLMGWAYALILGGALGNG
                     ADRLFSGYVVDFVLVHYGSWHFPAFNVADSALTVGAAIWIGSILLDYLADRRDGQDPD
                     EGPTA"
     gene            3857..4303
                     /locus_tag="AC16PP_14070"
     CDS             3857..4303
                     /locus_tag="AC16PP_14070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR6482801.1"
                     /translation="MVSSSDRRIDDNCRVTLHFAVLLESGEEVDTTRRSRPATFVIGD
                     GSLLPGFERALMGMKAGDDAQIPLEPGEAFGERQEGNVRRLPAKAFSGIDLEPGLLVS
                     FAAPEGELPGLVKCVDGSTVQVDFNHPLAGRRIIFDVSILRVEDVI"
     gene            4481..4933
                     /locus_tag="AC16PP_14075"
     CDS             4481..4933
                     /locus_tag="AC16PP_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR6482802.1"
                     /translation="MNGFTLVELMVAVAIMAIIAAVALPFYNQYSQRTYRTEAQADLL
                     ACAQAIERMAAVSFSYENAADTDADGVGDGNAGPVATALCDPLSVERGRYNITVNSTV
                     ATFDLTATPVGMMAGDGILTFDSTGARGWDQNGDGDTLDADEQNWEEE"
     gene            5087..5602
                     /locus_tag="AC16PP_14080"
     CDS             5087..5602
                     /locus_tag="AC16PP_14080"
                     /inference="COORDINATES: protein motif:HMM:NF023444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HIR6482803.1"
                     /translation="MTNKLNNGGFTVVELIVTLAVAAILFGFALPAFNELMSQRAMTA
                     RVNEFVLAVNYARSEAAKLGGVVSIQSMDDGDNGNEWGAGYCVVVGNPGNCDAPVLQV
                     FDAADDATLNGSGAFDSVGTLSFNSRGMLIGGVPGEIQLCSTDAGEDPGRTVFVNAIG
                     RASTAELVCNG"
     gene            5599..6117
                     /gene="pilV"
                     /locus_tag="AC16PP_14085"
     CDS             5599..6117
                     /gene="pilV"
                     /locus_tag="AC16PP_14085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HIR6482804.1"
                     /translation="MKDRVRGFSMIELLVAVLVMGVGVLGVTALQLVSMQNNRSALVR
                     AEAVQLAYDMMDRIRANPSGAVPGQAYNGLAFGDGPPAATDCMANNCSEAQMVAFDQA
                     VWKCSLGGFSDAGVCTNLRDGGVLPTVNDQPGLPNGDGAIAVDGGGIITVTVQWQEPN
                     AQIQSFAIDSQG"
     gene            6121..7245
                     /locus_tag="AC16PP_14090"
     CDS             6121..7245
                     /locus_tag="AC16PP_14090"
                     /inference="COORDINATES: protein motif:HMM:NF027400.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HIR6482805.1"
                     /translation="MKITFKENAVVGTQTRSAGFSLVELLLALALGLVVVAGIVQLFV
                     GNSQTSAIVNGQARLQENARFAFDFISRAARQAGYLGCAHVPDNIVKNLNGPWNLIPE
                     YNLTRIVDGYEAGAGGTWTPSIDTVPGGLVGNVNVAAGAIDTGVIAPGTDVVVFRNVQ
                     QPGQKLVQTLQPLGNPVVTAPGGDPGFGVGDVVLVANCEQAAMFRVTALAVAGNEATV
                     LRATGGSFFENAAFVNSPNGTVPATLSFLGRSYGPEATIGAVESTYFFIAPSVRQDNR
                     GNTPLALWQKVGAAGPVELIQGIEDLQVLYGIDTTLNDGIANPNRYVPFDSVPLDVGQ
                     IVAVRATITVNSVDVVTEDGNRLRRTFSKTIQIRNSNPEV"
     gene            7248..7808
                     /locus_tag="AC16PP_14095"
     CDS             7248..7808
                     /locus_tag="AC16PP_14095"
                     /inference="COORDINATES: protein motif:HMM:NF025061.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX family protein"
                     /protein_id="HIR6482806.1"
                     /translation="MGAIRQPSLSSPRQNTGVALFISLVLLLLLTIIGVSAVQTTTLE
                     VRMARNEHDSLLAFQAAESALRDAEAALDLLTSTVGFTDAGANGLWTVADLGDPDRWT
                     LGGVWDGGQSVQSATNIDGVNAQPRYIVEYVASVIRDENAYQLEDPYGASNSDRIEIF
                     RVTARGVGGTANAVVLLQSTYGRILD"
     gene            7877..>8843
                     /locus_tag="AC16PP_14100"
     CDS             7877..>8843
                     /locus_tag="AC16PP_14100"
                     /inference="COORDINATES: protein motif:HMM:NF037016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR6482807.1"
                     /translation="MSRSTIGWIGSIGLLLLSAASFAASPVPVSPSSTYTTAEDSAPF
                     AGYLNNVFSDADEPGDVLTYGLASTSGDVVINAVVDPVTGVVTFTPIPNANGTQTWVF
                     TATDTTGNVGEFTFTATVTPVEDAPVILVPDLVVTVSEDTASSSTDLLSVFSDADVAT
                     NGDSLTFSIESSPGDPVDAVSIVGSNLVITYVPELNGTETVDVRATDLAGNWAQLTVG
                     IVVNSVNDVPVIVGSVSDVLEAEDSPGSTVGFGGVFDDIDIDTNGDSLTLSVSAVSHG
                     AIASASMSGDTLNITYLPDQNGNGTVTVRATDSAGASVEYEVNVTV"
BASE COUNT         1464 a         2709 c         2918 g         1752 t
ORIGIN      
        1 aagatccagg cggcccgtat tgccgcacgc tccggggccc acaccgtgat taccagcggc
       61 agaatcgagg gcgtgctgaa ccgcgtcctc gaaggggccg atgtgggcac gctgctgctc
      121 gcttcgatga acccgctcga tgcgcgcaaa aggtggatcg ccggtcagct caaggtgaag
      181 ggcgagctgg agctcgacag gggcgcggtc gaggcgctgc gccgccgcgg ggtcagcctt
      241 ctgcccgtag gggtgtctgc cgtgcgggga cggttcgtgc gcggtgacgt ggtccgctgc
      301 gtggacggcg acgggctggc gatcgctcag gggctggtca attacagcag cgaggaggct
      361 gcccgattgc tgcgggccgg gtctgacgag attgccgaca ggttggggta cgcggcggag
      421 ccggagctcg tgcaccggga caatctggtg ctgctctccg gtgggtcgaa gccctgatca
      481 ggctttcagg gcggcgactt tggcgttcag gcgcgacttg tgccgggctg ccttgttctt
      541 gtgcatgatg cccttggaag ccatccggtc gatgacgggt atcgcatcgt tcagggctgc
      601 gctggccgcg tcgtactccc ggctctggat ggccgcgtcg acgcgcttga tgtaggtgcg
      661 caccatggaa cgctggctgg cattttgtcg ccgccgtttc tcggcctggc gtgcgcgctt
      721 gcgtgcttgt ggactgttgg ccaagcgtgg ctcctggggg tccggattgc gaaggcgcgg
      781 taatatgccg ccgaggtatg gtgatgtcaa ccggcactga gaaacccgag cagcaggccg
      841 cggccttgga aggtccccag aacgtggccg cacagggagg catcgtttcg ggcatgaccc
      901 tgctgtcccg aatcaccggg ctggttcgcg acatggcatt gtcctacttt ttcggggccc
      961 aggacgtcgc ggatgcgttc ttcgttgcct tccgcatccc caactttttc cgtcggctct
     1021 tcgccgaagg cgcgttcagc caggcgttcg tgccggttct ggccaggtac agagggggtg
     1081 gcgtaggcga gctgcgacgc ttcgtcgcgg tgatgcaggg gaacctcata gccgccttgt
     1141 cagtctttgt gctgctgggc gtgattttct cgcccgcgct ggtcgccgtg ttcgcgcccg
     1201 ggttcttcgg cgacgacgaa cgcttcgcgc tcaccagcag catggttcgc atcacgttcc
     1261 cctatctcgg cctcatctcg ctcaccgcat tcgccggggc gctcctcaac agccaccacc
     1321 gctacgccgt gccggcgttc acgcccgtcc tgctcaacct gagcctcatt gccgcggtgc
     1381 tctttgctgc cgacgtcttc gcgacccccg tgttcgccct ggcatggggt gttctggtcg
     1441 ccgggttcgc tcagctcgcc tttcagggcc cctcgctcag aaggctcgga ctgctcgtgg
     1501 cgccccggat cagccggacc gaccccgggg cccggcaggt cggcaagctg ctcattccgg
     1561 cagtgctcgc ggcgtcggtc aatcagatca acgcgctcat cgataccatg ctcgcctcga
     1621 cgcttgttac ggggagcatc tcgtggctgt actactccga ccggctgctc gagctcccta
     1681 tcgggctggt ggccgttgct ctgggcacgg tgctcctgac gacgctgtcg cgcctggatt
     1741 cctcaggcga ccgggccggg ttcgccgcga ccgtggactg gggcatgcga atgggggcga
     1801 ttctcggcgt gccggcggca gccgcgcttt acgtgcttgc ggttccgctg gttgcgagca
     1861 tttttctgcg cggcgcgttg acccctctcg atgcccgcat ggcctcgctc agcctgcagg
     1921 cgtttgccgt cggcctgccg gcgctcgtgc tcgtcaaagt gctggctccg gcgtacttcg
     1981 cgcgccggga caccgccacg ccgttccgaa tcggcgcggt cgccgtgggc gtcaacgtgg
     2041 cgctgaatct ggcgctcttc ttctggatgg ggcacgtggg cctggcgctg gcgacgtcgg
     2101 cttccgcgtg ggttaatgcg ctgatgctgc tgcgcgggct cgtctccggt gacgcttacc
     2161 gcccgggccg gccgttcctg gtgagtacag caagggctct ggccgcgtcg gccgtcatgg
     2221 tggccgcttt cgcctactgg gttccggagc cttcggcctg gatggccgcg cccgatggcg
     2281 agcgcgtcgt ctggctggcg ggcgtggtgg cgggcggtgg cagcgtgtac ctgctttgcc
     2341 tcatcgtttt cggcgagcgg ccgcggaacc tgctccatcg ggcgtgaggc gcctataatg
     2401 gtgcgccatg gaaatcatca gaggcctcca caacgtcaaa ccccgccatc gtggctgcgt
     2461 gctcacgatc ggcaacttcg acggtgtgca ccacggccac caggtcctgc tcgcccatct
     2521 catcgcgaaa agcgaggagc tgggcgtgcc gagcatgctc atgacgctcg agcccgcgcc
     2581 cagagagttt ttcgccgggg aaacggttcc cgcccggctg acccggtttc gcgagaaggt
     2641 cacgctgctg gaacccacag ggctccacag gatcctatgc cttcccttca acgagcgcat
     2701 cgccagcatc ccggccgaat ggatcgtgga ggagctgctg cacaaccagc tgggcatccg
     2761 ctacatcgtg gtgggtgacg acttcaggtt cgggaagggc gccgcgggcg actatcggat
     2821 gcttcaggca gccggggagc gttacgggtt cgaggtcagt cacatcggca cgttgacgtt
     2881 cgatcatgaa cgagtcagca gcacgcggat cagggagtgt ctgtcggcgg gagacttcgc
     2941 gctggcggaa aagctgctgg gccaccctta cttcatcatg ggacgggtgg tatacgggcg
     3001 gcagctcggg cgcaagctgg ggattccgac cgcgaacatt cagctgcagc gctatcgggc
     3061 cgccctggag ggcgttttcg cggtgagcgt gcgaggcctg gagaaggagt atcagggcgt
     3121 cgcaaacgtt ggcattcgtc cgaccgtgga cgggcgcgag ccgctgctgg aggtgcacat
     3181 attcgacttt gacagggaca tatacggaac gctgctcacc gtcgagttcc gtcacaagat
     3241 cagggacgag attgcctttg acggccttga tgctctcaag gcgcagattc aagaggacca
     3301 gcgtacggca agggcgtggt ttgccaatga atgaaaagat gggacgtgcc gggatgctga
     3361 agtggctggg gctcagcgcg atcgtggtgg tgctggatca ggcgaccaag tggtggatcg
     3421 tccaggagtt tcgtctgggt gagcgcatgg actggcttcc gttcttgagc tgggtgcgat
     3481 ggcacaacga cggggcggct ttctcgttgc tcagcggcgc cggcggacgc tggttcttcg
     3541 ttgccctcgc cgtcgcgttc gccgtgttca tcatctggga gctcaggcgc cttccggtcg
     3601 gcgggcggtt gatgggctgg gcctacgcgc tgattctcgg cggcgcgctc ggcaacggcg
     3661 ccgatcgcct tttttccggc tacgtcgtcg acttcgtgct ggttcactac ggctcatggc
     3721 attttcccgc gttcaacgtg gcggactccg ccctcacggt gggtgccgcc atctggattg
     3781 ggtcgatact tctggattac ctggcagacc ggcgtgacgg tcaggatccc gatgaagggc
     3841 cgaccgcgtg agcctcattg tcagctcgtc ggatcggcgc atcgacgaca actgtcgggt
     3901 aacgctgcac tttgccgtgt tgctggaatc cggcgaggaa gtggacacca cccgtcggtc
     3961 ccgaccggca accttcgtca tcggcgacgg tagcctgctg cccggattcg agcgggcgct
     4021 catgggcatg aaggcagggg acgatgcgca gatccccctg gaacctggcg aagcgtttgg
     4081 agagcgccag gaaggcaatg tccgtcggct gccggcgaag gcgttttccg gaatcgatct
     4141 ggagcctggg ctgctggttt cgttcgcggc tccggaggga gaattgccgg ggctcgtcaa
     4201 atgtgttgac ggcagcaccg ttcaggtgga cttcaatcat ccgctggcag gccgcaggat
     4261 catcttcgac gtgtccattc tccgggtcga agatgtgatc tgatccgccg tcgtttctgg
     4321 gccggacgcg tatcctacag atcatcgctc caatcctacc gctcagcgcc gttggatagt
     4381 ttgtcgacgt cgcgggcgcc tatgattgat gggcccgaac gatcaggatc cgaaaatgcg
     4441 ttcatccggt cccgtggaaa caccagacga tcgatcacgt atgaacggct tcactctggt
     4501 ggagctgatg gtcgcggtgg cgatcatggc gatcatcgcg gccgttgccc tgcctttcta
     4561 caaccagtac tcgcagcgca cgtaccgcac cgaagctcag gcggatctgc tcgcctgcgc
     4621 ccaggccatc gaacggatgg ccgcggtgtc gttctcctac gagaacgcgg cggatacgga
     4681 cgctgacggc gtcggggacg gcaacgcagg ccctgtcgcg accgccctgt gcgacccgct
     4741 gtcggtcgag cggggccgct acaacatcac cgtcaactcg accgtagcta cgttcgatct
     4801 caccgcaacg cccgttggaa tgatggccgg tgacggcatc ctgacatttg attccacggg
     4861 tgcgcgcggg tgggatcaga acggcgacgg cgacaccctc gacgcagacg agcagaactg
     4921 ggaagaagag tgagatcagg gtcaccctga gcatcgtctc cccgcactga ccgcttatcc
     4981 tgacaaatca acccgctcat cgggcgccgc gttcatttga ccgagcgcct tcgtagactg
     5041 tcagcgccga aactaaaagc tgaattttgg cgcgattgag gacgcaatga caaacaagtt
     5101 gaacaacggt ggtttcactg tcgtggagtt gatagtcacg ctggctgttg ccgcgatcct
     5161 gtttggcttt gccctgccgg ccttcaacga gctcatgagc cagcgcgcaa tgactgcccg
     5221 cgtcaacgag ttcgttctgg ccgtcaacta tgcgcgcagc gaggcggcaa agctcggcgg
     5281 cgtggtcagc attcagagca tggacgacgg cgacaacggc aacgagtggg gcgccgggta
     5341 ctgcgtggtg gtcgggaatc ccggcaactg cgatgcgccc gtcctgcagg tgttcgatgc
     5401 ggcggacgac gccacgctga acggcagcgg cgcgtttgat tcggttggca ccttgagctt
     5461 caactcgcga gggatgctga tcggcggcgt gcctggagaa attcagcttt gcagcacgga
     5521 cgcgggagag gaccccggca gaacggtctt cgtcaacgcc attggaagag cgtctacagc
     5581 ggagttggta tgcaacggat gaaagatcgt gttcgcggat tttccatgat cgagctgctg
     5641 gtcgcggttc tcgtcatggg agtaggagta cttggggtga ccgcattgca gctggtcagc
     5701 atgcagaaca accgtagcgc gctggtacgg gcagaagccg tccagctcgc ctacgacatg
     5761 atggacagaa taagagcgaa cccttcgggg gctgttccgg gacaggcata caacggcctg
     5821 gccttcggag acgggccgcc ggctgcaacg gattgcatgg cgaacaactg cagcgaggct
     5881 cagatggtgg cattcgatca ggcagtctgg aagtgctcgc tgggcggctt ctcagatgcc
     5941 ggagtgtgca ccaacctgcg cgacggcggt gttcttccga cggtcaacga tcagcccggg
     6001 ttgcccaacg gcgacggggc cattgccgtc gacgggggtg gaatcatcac cgtcaccgtg
     6061 caatggcagg agcccaacgc gcagatccag agcttcgcca tcgacagtca gggctgattt
     6121 atgaaaatca ctttcaagga aaatgccgtg gtcgggacgc agacgcgaag tgctggattc
     6181 tcgctggtcg agcttttgct ggctctggcg ctcggtctgg tggtcgttgc cggcatcgtg
     6241 caactgttcg tcggcaactc gcagacctcg gccatcgtca atgggcaggc gaggctgcag
     6301 gaaaacgccc gattcgcttt cgacttcatt tcccgcgccg cccgccaggc cggctatctc
     6361 ggctgcgcgc acgtgccgga caacatagtc aagaacctga atgggccgtg gaacctcatt
     6421 ccggaataca acctgacccg catcgtcgac ggctacgagg ccggcgcggg cggtacctgg
     6481 acgcccagta tagacacggt gcccggtggc ctcgtcggta acgtgaacgt cgccgccgga
     6541 gccatagaca ctggcgtgat cgcgccgggt acggacgtcg tcgtgttccg aaacgttcag
     6601 cagcccgggc agaagctcgt gcagacgctg cagccgctgg gcaaccccgt cgttaccgct
     6661 cccggcggag accctggttt cggtgtgggt gacgtggtgc tggtcgccaa ctgcgagcag
     6721 gccgccatgt tccgggtaac cgcgcttgcg gtggcgggta acgaggcgac ggtgctcaga
     6781 gcgaccggcg gcagcttttt cgagaacgcc gcgttcgtca actcccccaa cggcacggtt
     6841 ccggcgacgc tgtcgttcct cggccgctcc tacggtccag aagcgaccat cggcgcggtg
     6901 gaatccacct acttcttcat cgcgcccagc gtccgtcagg acaatcgggg gaacacgccg
     6961 cttgcgcttt ggcagaaagt cggtgcagca gggccggtag agctcatcca gggcatcgaa
     7021 gatctgcagg tcctctacgg catcgatacc acgctcaacg acgggatcgc caaccccaat
     7081 cgctacgtgc cgtttgattc ggtgccgttg gatgtcggac agatcgttgc ggtgcgcgcc
     7141 accataaccg ttaacagcgt tgacgtggtc accgaggacg gtaaccgact gcggcgcacg
     7201 ttcagcaaaa ccattcagat cagaaactcg aacccggagg tctagccatg ggagcgatta
     7261 gacaaccctc gctttccagc cccaggcaga acacgggtgt cgccctgttc atcagcctgg
     7321 tgctcctgct gctcctgacc atcatcggtg tttcggccgt gcagaccacg accctcgagg
     7381 tgcgcatggc gcgcaacgag cacgattccc tgctcgcgtt tcaggcagcc gaatccgcgc
     7441 ttcgggatgc cgaggccgct ctggatctgc tcacgtccac cgtgggtttc accgacgcgg
     7501 gcgccaacgg tctgtggacg gtggccgacc tcggcgatcc tgaccgctgg acgctaggtg
     7561 gcgtctggga cgggggccag agcgtccagt ctgccacgaa catcgacggc gtgaatgctc
     7621 agccgcggta catcgtcgag tacgtggcct cggtgattcg cgacgaaaac gcctaccagc
     7681 tggaagatcc ttacggcgcg tccaactctg accgcatcga gattttccgc gtcaccgcgc
     7741 gcggcgtcgg cggcaccgcg aacgccgtcg tgctgcttca atccacctat ggacggattc
     7801 tggattgaag cgactctcaa ccattcgaat gttgccgttc gcgtcgtttc gtagtcggcg
     7861 cacaggaggt tccaggatgt ctcgcagtac tatcggttgg atcgggtcga tcggcctgct
     7921 tttgctatca gcagcgtcgt ttgccgcgtc tcccgtgccg gtatcccctt cctctaccta
     7981 cacgacagcg gaagattcgg ccccatttgc tggctatctg aataacgtct tctccgatgc
     8041 ggacgagcct ggcgacgtcc tgacctacgg ccttgcctcc acgagcggcg atgtggtgat
     8101 taacgcggtg gtcgatcctg tcaccggcgt ggttaccttc accccgatcc ccaatgcgaa
     8161 cggcacccag acctgggtgt tcacggccac cgacactacc ggcaacgtcg gtgagtttac
     8221 gtttacagcg acggtgacgc cggtggaaga tgcgcccgtc atactggtgc cggatctggt
     8281 cgtgacggtc agcgaggata cggcgagctc gtcgaccgat ctcctgagcg tgttcagcga
     8341 cgcggacgtc gcgacaaacg gcgactcgct gacgttctcc atcgagagtt ccccgggaga
     8401 cccggtcgac gcggtaagca tcgtcggctc gaatctggtc atcacgtacg ttcccgagct
     8461 caacggcacg gagacggtag acgttcgggc aacggactta gctggcaatt gggctcagct
     8521 gaccgtcgga atcgtcgtca attcggtaaa tgacgttccc gtcatcgtgg ggtcggtttc
     8581 cgacgtgctg gaggccgagg attcccccgg atccacggtc gggttcggcg gcgtgtttga
     8641 cgatatcgac atcgacacca acggggactc cctgaccctg agcgtgtcgg ccgtctccca
     8701 cggcgccatt gccagtgcgt ccatgtcggg cgacaccctg aacatcacct atctcccgga
     8761 tcagaatgga aacgggacgg tgaccgtgcg cgccaccgac tcggcaggag catccgtcga
     8821 gtacgaggtc aacgtcactg tca
//
LOCUS       DBNHKV010000432         6315 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1406037, whole genome shotgun sequence.
ACCESSION   DBNHKV010000432 DBNHKV010000000
VERSION     DBNHKV010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1406037"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="AC16PP_14105"
     CDS             complement(<1..431)
                     /locus_tag="AC16PP_14105"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6482808.1"
                     /translation="MASDQASGASARPDAAHSGPARPLSRRTLFYYGLADMPIQMAGI
                     PVAAFIPNYYGQDLGVSLGAVGTIWLLTRLFDAVSDPLIGWLSDRTDTRWGRRRVWMV
                     AAVPILMLSVYKLFMPDPAAVSAGYLLGWLVALWVGWTMLF"
     gene            575..2506
                     /locus_tag="AC16PP_14110"
     CDS             575..2506
                     /locus_tag="AC16PP_14110"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HIR6482809.1"
                     /translation="MKARAFVAAILVLTGASAGARENVAWPAYGGGPGGGHYSQADQI
                     TPDNVAGLEVAWQHRSGDYREGTIRGVEGFSGDAVAASAFIGTPIVVNDTLYYCTPFN
                     RVFALDPQTGAERWMFDPGVKHELEHLTNCRGVSSWTDPLASPDALCAHRIIAPTLDG
                     RIFAVDGKTGKRCEGFGRNGEIDLTEGLTEHGAQEYGITSAPAILNDILVTGSYVTDS
                     IRIDIPSGVVRAYDLRTGAFRWGWNPVPPGTAERDEAGNYRAGTTNVWSTISVDAERN
                     LVIVPTGNSSPDYYGGHRDGDLDYYSSSVVALDGDTGEVVWHYQTVHHDIWDYDLPAQ
                     PTLLDVTIDGKTRPAVVQVTKMGMTFVLDRETGQPLFPVEERPVPQEGAAPGEYLAPT
                     QPFPLKPEPLHKLGISPDDAWGFTFLDRGWCRDALEAMTTGPIYTPISVNKETPLYPS
                     VIGGQNWGAPAVDPERQIMVANTHHVAMILNLIPRDQCDGTENFPQEGSPYCATLRPF
                     LSRFGAPCTKPPWGTLSAVNLSTGDIEWQVPLGNLKYQAFWPFTMMKGGVQMGGPTIT
                     SAGLIFIAASADRHVRAFDLDTGEELWADEMPTTGNAVPMTYVSGGRQYVVIAAGGHF
                     TSPSPPADYLIAYALPEKD"
     gene            complement(2513..3163)
                     /locus_tag="AC16PP_14115"
     CDS             complement(2513..3163)
                     /locus_tag="AC16PP_14115"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6482810.1"
                     /translation="MKGLTERQLKRRERILAAARKLITDRGYDGLTMRDLAAESGVAP
                     KTLYHQFHSKENLLRVAVEERYRYLYQIIDDEQIEHGIDRLFFIIDTVASTTENNLAY
                     AKALRPLLTSETSSTFAAIRMNTYRKAIDQIEAEGEVVDWIDVDILAAVVYRQVNPIY
                     LAPWYKETPWATVARIAKLDICLILAAVTTGYTQTTAEKTIRDLQKQLKRSGSRYI"
     gene            complement(3186..3917)
                     /locus_tag="AC16PP_14120"
     CDS             complement(3186..3917)
                     /locus_tag="AC16PP_14120"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6482811.1"
                     /translation="MQEFHVNVATPDGEMECFAAHPDGEGPFAAVILYMDAPGIREEL
                     RDFCRRIAGQGYFCLLPDLYYREGRVRFDLRKGEEELKRMFAVGSRLTVEAVMRDTGG
                     MLAYLEDNPLARSPTGCIGYCMSGQFVVAAAGTFPGEIAAAASLYGTRIVTDKPDSPH
                     KLAERMKGELYLGFAEHDHWVEDFVIPKLIEELDQHGVKYELEVHPGTEHGFCFPERP
                     SYVEAAAEKVWDTVFELFDRRLNAR"
     gene            4091..5821
                     /locus_tag="AC16PP_14125"
     CDS             4091..5821
                     /locus_tag="AC16PP_14125"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR6482812.1"
                     /translation="MHLDLVIRNGMLIDGRRTPSYRADVGIRDGKVAVIGRIAPGSGD
                     RELDADGLIVAPGVVDLHTHYDSQIFWDPWCRISGQHGVTSVVVGNCGFGFAPVRPEH
                     RDRAMLTMARNEAVPLECMQQGMPWDWTTYPEFLDSLDRTPKGVNVLSYVGLNPLMVW
                     LTGSVEEAKGRPLTRAEKAEAERLLAEAMDAGACGWSAQFIGADTVQRDHDGTPMITD
                     LMSREDIEFFAAALGRLGRGGIQMIGPPKMADVLAAASGRPVIYNVLAARSDQHGSPA
                     ESYKDVIAWLARANAEGNRIIAHAVTCAIDYQFTMEDWNLFDSSPLWRELTIGTVPER
                     KAKMMDAQRRAALHEEWAAGKAPIAGGGTEQREERIGVKFQDLVLAYVTEEGEHLRSW
                     EGHTVAEIAAGMGVHPIDAFLDVSIACDLKAGWQSEPRTIDPQVMRDIANCPYAVPGL
                     SDGGAHTKFLTTGTYPTEFLATWVRDNGVMDLEEAHWRLSAYSAQTAGLTDRGFIAEG
                     MPADIVVYDLQALELLPSERVADWPGGAWHLDRRANGWRWTIVNGAVTFADGVCTGAT
                     PGRLLRHGHA"
     gene            5818..>6315
                     /locus_tag="AC16PP_14130"
     CDS             5818..>6315
                     /locus_tag="AC16PP_14130"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6482813.1"
                     /translation="MNTVPATAASVALDPLTQTLSDAAEACFVRHGVAKTTMSHVAEE
                     AGVSRTTLYKRFAVMEEVLQAVFIREFDRFEARLAGNLEPIDDPGERLVEIVVAIAEN
                     VPENAGLARLLDGSRTRTENMALAAGRRALNDRVIDMIGDPLDGLARAGVLRGDVERG
                     ELIEWI"
BASE COUNT         1095 a         2005 c         2128 g         1087 t
ORIGIN      
        1 atgaacagca tggtccagcc cacccagagc gccaccagcc agcccagcag atagcctgcg
       61 ctcaccgcag caggatccgg catgaagagc ttgtagaccg acagcatgag tatgggcact
      121 gctgccacca tccaaacgcg tcggcgcccc caccgcgtat ccgtgcggtc ggacagccag
      181 ccgatcagcg gatccgatac cgcatcgaac aggcgcgtga gaagccagat ggtcccgacc
      241 gccccgaggc tgacgccgag gtcctggccg tagtagttgg ggatgaaggc ggccacggga
      301 atgcccgcca tctggatggg catgtcggcg agcccgtagt agaacagggt tctgcgcgac
      361 agcggccgcg ccgggccgga gtgcgcggca tcaggccgtg ccgatgcgcc ggacgcctgg
      421 tcgctggcca aatggatctc ccctcaccct gaaaagcgga tgcaccacgt cccgccgagt
      481 ataacgtgtt ttccttttga tctgcggcgg agcgggtggt tgaatgaggc tcagcgcagg
      541 ctggagccgc gcgatgatcg ggaggggatt caccatgaaa gccagagctt tcgtcgctgc
      601 cattctggtg cttacgggcg catccgcggg tgctcgggag aacgtcgcct ggccggccta
      661 cggtggcgga cccggaggcg gtcactactc ccaggccgac cagatcacgc cggacaacgt
      721 ggcgggcctg gaggtcgcct ggcagcatcg ctcgggcgac tatcgagagg gcaccatccg
      781 cggcgtggag ggcttcagcg gcgacgcggt ggccgcctcg gccttcatcg gcacgcccat
      841 cgtcgtgaac gacacgctgt attactgcac gccgttcaat cgggtttttg ccctggatcc
      901 tcagacgggc gccgagcgtt ggatgttcga cccgggcgtg aagcacgagc tggagcacct
      961 caccaactgc cgcggggtga gctcctggac ggaccccctg gcgtctcccg acgccctttg
     1021 cgcccaccgc atcatcgccc cgaccctgga cggccgtatc ttcgccgtgg acggaaaaac
     1081 gggtaagcgt tgcgaaggct tcggccgcaa cggcgagatc gatctcaccg aggggttgac
     1141 cgagcatggc gcccaggaat acggcattac cagcgcgcca gccatactca acgacattct
     1201 cgtcacgggc tcctacgtca cggacagcat ccgcatcgac ataccctcgg gcgtcgtgcg
     1261 tgcctatgac ctgcgcaccg gagcgttccg ctggggttgg aaccccgtac cgccgggcac
     1321 cgccgagcgc gacgaggcgg ggaactatcg cgcgggcacc accaacgtct ggtccaccat
     1381 cagcgtggac gccgaacgca acctggtgat cgtgccgacg ggcaactcgt cgcccgacta
     1441 ctacggcggc caccgggacg gcgacctgga ctactactcg agctccgtgg tggcgctgga
     1501 cggcgacacc ggcgaggtcg tctggcacta ccagacggtg catcacgaca tctgggatta
     1561 cgacctgccc gcgcagccga ccctgctgga tgtcaccatc gacggaaaga cgcgtccggc
     1621 cgtggtgcag gtgaccaaga tgggtatgac gttcgtgctc gatcgggaga ccgggcagcc
     1681 gctctttccc gtggaggagc ggccggtgcc tcaggagggt gcggcgcccg gcgagtatct
     1741 tgcgcctacc cagcccttcc ccctcaagcc cgagccgctg cacaagctgg gcatctcccc
     1801 cgacgatgcg tggggcttca cgttcctgga caggggctgg tgtcgggatg cgctcgaggc
     1861 catgaccacc gggccgatct acacgccgat ctccgtgaac aaggaaaccc cgctgtaccc
     1921 gtcggtgatc ggcggtcaga actggggcgc gccggcggtg gatccggagc gtcagatcat
     1981 ggtggccaat acccaccatg tggccatgat tctcaatctg atcccgcggg atcaatgcga
     2041 cggcacggag aactttcctc aggagggctc gccctactgc gcgacgctgc ggccttttct
     2101 gtcacgcttt ggcgcgccgt gcaccaagcc gccgtggggg accttgtcag ccgtgaacct
     2161 gtccacgggc gacatcgagt ggcaggtgcc gctgggcaac ctgaagtacc aggcgttctg
     2221 gcctttcacc atgatgaagg gcggggtgca gatgggcggc ccgacgataa cctcggccgg
     2281 gctgatcttc atcgccgcgt cggccgaccg gcacgtccgt gctttcgatc tcgataccgg
     2341 cgaggagctt tgggccgatg agatgccgac gacgggcaac gccgttccca tgacgtacgt
     2401 ctccgggggc cgacagtacg tggtcatcgc ggcgggcggg cacttcacct cgccgtcgcc
     2461 gcccgccgac tatctcatcg cctacgcgct cccggagaag gactgagggg gctcagatgt
     2521 agcgggaacc ggatcgtttg agctgcttct gcagatcgcg aatggtcttt tcggccgtcg
     2581 tctgcgtgta gccggtggtc accgcggcca ggatcaggca gatgtccagc ttggcgattc
     2641 tggcgacggt ggcccagggt gtctccttgt accatggggc gaggtaaatg gggttcacct
     2701 gccggtagac caccgccgcg aggatgtcga cgtcaatcca gtcgacgact tccccctcgg
     2761 cttcgatctg gtcgatggct ttgcggtacg tgttcatgcg gatggccgca aacgtgctgg
     2821 aggtctcgga cgtaagcagt ggcctgagcg ccttcgcgta ggcgaggttg ttctcggtgg
     2881 tgctcgccac cgtgtcgatg atgaagaaca gccggtcgat gccgtgctcg atctgctcgt
     2941 cgtcgatgat ctgatacaga taccggtagc gctcctccac cgccacccgc agcaggttct
     3001 ctttgctgtg gaactggtgg tagagcgttt tcggcgcgac cccgctctca gcggccagat
     3061 cgcgcatggt cagtccgtcg tagccccgat cggtgatgag cttgcgggca gcggccagga
     3121 tccgctcacg ccgtttgagc tggcgctcgg taagaccctt catgcagcgt tccgcacccg
     3181 tcgcctcatc tggcattcag ccggcggtcg aacagctcga agacggtatc ccagactttc
     3241 tccgccgcgg cctccacgta ggatgggcgc tccgggaagc agaagccgtg ctccgtgccg
     3301 ggatgcactt cgagctcgta cttcacccca tgctggtcga gctcctcgat gagcttggga
     3361 atgacgaagt cctcgaccca gtggtcgtgc tcggcgaagc cgaggtacag ctcacccttc
     3421 atgcgctcgg cgagcttgtg gggtgagtcg ggcttgtcgg tgacgatgcg ggtaccgtaa
     3481 agcgacgcgg cagcggcgat ctctccggga aaggtgcccg ccgccgccac gacgaactgg
     3541 ccgctcatgc agtagccgat gcagcccgtc ggcgatcgcg ccagagggtt gtcttccagg
     3601 taggcgagca tcccgccggt atcacgcatg accgcctcca cggtgagccg cgagccgacg
     3661 gcaaacatgc gcttgagctc ttcctcgccc ttgcgcaggt cgaagcggac gcggccctcc
     3721 cgatagtaga gatcgggaag cagacagaag tagccttggc ccgcgatgcg ccggcagaag
     3781 tcgcgcagct cttcgcgaat acccggcgcg tccatgtaaa ggatgacggc ggcaaacggg
     3841 ccttcgccat cggggtgcgc ggcgaagcac tccatctcgc catccggagt ggccacattg
     3901 acgtgaaatt cctgcatcgt gttctcgttt ttttcgcggg atgagggcaa cgagcataaa
     3961 acgtgggtcg gaacctcaca atggcggtaa cgcgtggtaa ttccagacca agaaacccgg
     4021 cccgccgcat tgacggcaag tgaacagatt tgataatttg ttcagattcg gccgctgcag
     4081 ggatcaacca atgcatctcg atctcgtcat ccgtaacggt atgctcatcg atgggcgccg
     4141 cactcccagc tatcgcgccg acgtcggcat ccgcgatggg aaggtcgccg tcatcgggcg
     4201 gatagcgccc gggagcggag atcgcgagct cgatgccgac gggctcatcg tggcgcccgg
     4261 cgtcgtcgat cttcataccc attacgacag ccagatcttc tgggatccct ggtgccggat
     4321 ttccgggcag cacggagtca ccagcgtggt ggtgggtaac tgcgggttcg gcttcgcgcc
     4381 ggttcggccc gagcatcgcg acagggccat gctcaccatg gcgcgtaacg aagccgtgcc
     4441 gctggagtgc atgcagcagg gcatgccttg ggactggacc acctatccgg aatttctcga
     4501 cagcctcgat cgcaccccga aaggggtgaa cgtgctctcc tacgtggggc tcaacccgct
     4561 gatggtgtgg ctcacgggct cggtggaaga ggcgaaaggc cggccgctga cccgggcaga
     4621 aaaggccgag gcggagcgcc tgcttgccga agccatggac gccggcgcct gtggctggtc
     4681 ggctcagttc atcggcgccg acaccgtcca gcgcgatcac gacggcacgc ccatgatcac
     4741 cgacctcatg agccgcgagg acatcgagtt cttcgccgcg gcgctgggtc gtctgggccg
     4801 cggcggcatt cagatgatcg gtccgccgaa gatggcggac gtgcttgcgg cggcgtccgg
     4861 gcgtccggtg atctacaacg tgctcgccgc tcgcagcgat cagcacggct cgcccgcgga
     4921 gtcttacaag gacgtaattg cgtggctcgc ccgggccaat gccgagggaa accgcatcat
     4981 cgctcatgcg gtgacctgcg ccatcgacta ccagttcacc atggaggact ggaacctgtt
     5041 cgactcgtcc ccgctgtggc gggagctcac catcggcacc gtgcctgaaa gaaaggcgaa
     5101 gatgatggac gcccaacgtc gtgctgcgct ccatgaggaa tgggcggcgg gaaaggcgcc
     5161 catcgcgggc ggtggaaccg agcagcgaga ggagcggatc ggcgtcaagt ttcaggacct
     5221 ggtactcgcc tacgtcaccg aagaaggcga gcacctgcgg agctgggagg gccataccgt
     5281 tgccgagata gcggcgggga tgggcgttca tcccatcgac gcgttcctgg acgtatccat
     5341 cgcctgcgac ctcaaggctg gctggcagag cgagccgagg accatcgatc cccaggtcat
     5401 gcgcgacatc gccaactgtc cgtatgccgt gcccgggctg tccgacggcg gggcgcacac
     5461 caagttcctg accaccggaa cctacccgac ggagtttctc gccacgtggg tgcgcgacaa
     5521 cggggtgatg gacctggagg aagcgcactg gcgcctctcc gcctactccg cgcagacggc
     5581 ggggctgacg gaccgggggt tcatcgcgga gggcatgccc gcggacatcg tggtctacga
     5641 tctgcaggcg ctggagctgt tgccgtccga gcgcgttgcg gattggcccg gcggcgcgtg
     5701 gcacctcgac cggcgcgcga acggctggcg ctggacgatt gtcaacggcg cggtcacctt
     5761 tgcagacggc gtgtgcaccg gcgcgacgcc cgggcgcctg ctcaggcacg gtcacgcatg
     5821 aacaccgtgc cggccacggc ggcttcggtc gcgctggacc cgctcacgca gaccctgagc
     5881 gatgcagcgg aggcctgctt cgtgcgccac ggagtggcga agacgaccat gtcgcacgtg
     5941 gccgaggagg cgggcgtttc gcgcaccacg ctctacaagc ggtttgccgt catggaggag
     6001 gtgctgcagg cggtcttcat tcgcgagttc gatcggttcg aggcgcggct tgcaggcaac
     6061 ctcgaaccga ttgatgatcc cggtgagagg ctggtggaga tcgtcgtggc gattgctgag
     6121 aacgtgcccg aaaacgcggg gttggcccgc ttgctcgacg gctcgaggac gagaacggag
     6181 aacatggcgc ttgccgccgg gcggcgtgcg ctcaacgatc gggtgatcga tatgatcggc
     6241 gatcccctcg atgggcttgc gcgcgccggg gtgctgcgtg gggacgtgga acggggcgag
     6301 ctgatcgagt ggatc
//
LOCUS       DBNHKV010000433         6314 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_699830, whole genome shotgun sequence.
ACCESSION   DBNHKV010000433 DBNHKV010000000
VERSION     DBNHKV010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6314)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6314)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6314
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_699830"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="AC16PP_14135"
     CDS             <1..543
                     /locus_tag="AC16PP_14135"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR6482814.1"
                     /translation="GLQAEVTILDRSAQRLHWLRRHFNGRARVLMADGNAIGKAVETA
                     DLVVGAVLVPGAAAPRLVTREMVEAMANGAAIVDISIDQGGCFETSRPTSHDQPTYVE
                     AGVVHYCVTNMPGGVARTSTLALSKVTLPFVMELASRGWEDAFARDRHFANGLNVHRG
                     SIRHPEVAKALEHAVPPMAA"
     gene            complement(579..1517)
                     /gene="speB"
                     /locus_tag="AC16PP_14140"
     CDS             complement(579..1517)
                     /gene="speB"
                     /locus_tag="AC16PP_14140"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008369387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HIR6482815.1"
                     /translation="MTRSEHTRSDLLGHRSGMTFAGELSFLRRKYTRDIEGADLVVTG
                     LPFDMATTFRPGARLGPQAIRAASVQLAGIAGRAFPYGEDPFATLAVADCGDCFIDSG
                     YPQHGAAAIEAHARDIIARGCKLLSFGGDHFVTYPLLRAHAEKHGPMSLIHFDAHVDT
                     WEDDGSRIDHGSMFLRALREGLIVPERSVQIGIRSFSQQDHGFVVCDAPWVHEHGPQA
                     VLERVLSVVGQHPAYLTFDIDCLDPAFAPGTGTPVAGGLSSAQALHIIRGLTPVNLVG
                     MDVVEVAPAYDHAELTAIAAATIAHDLICQEALRRG"
     gene            complement(1519..2751)
                     /locus_tag="AC16PP_14145"
     CDS             complement(1519..2751)
                     /locus_tag="AC16PP_14145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6482816.1"
                     /translation="MRTYPGLDGSCEADVCIVGGGYTGLSAALELGRRNYDVVVLEAH
                     RVGSGASGRNGGVLGMGQRKDQLELESWLGADAARKLWRIACDANDLVRDRIAEFGID
                     CDLKSGELTAAHRERYEKTLWSYAEHLERDYGYSDRRIVSREEVAKMLGADTLYGGYV
                     DLRAGHVHPLNLAIGLARAASDHGATLHEQTPALGYRQEGDRVLVRTASGDVRARFLI
                     IACNGYLGRFEKGAERYQMPINNFILATEPLGAEMGRAINRDDLAVVDTRFVVNYFHN
                     SPDHRLIFGGGENYSSRFPKDIKAFVRRRMLQIYPQLESARIDFGWGGTLAITMRRMP
                     QFGRKGANVYWAQGYSGHGIAMANMGGKLVADAIMGQAEEFDTFAGLKHLPFPGGRYL
                     RWPALVSGMLYYAFLDRL"
     gene            complement(2823..4136)
                     /locus_tag="AC16PP_14150"
     CDS             complement(2823..4136)
                     /locus_tag="AC16PP_14150"
                     /inference="COORDINATES: protein motif:HMM:NF012348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR6482817.1"
                     /translation="MGKPSEMPDELELLLSDMNGVPRGKTLRGISYSEAHPPHLAEAI
                     FFQTITGGYAAAMETYDPNDRDLLLRPDWSTYVPTPWKPDTGQVICESLDQAGERLPY
                     DPRNVLRRVLDAYESMGLRPVVAPEVEFYLLEAVSSHDLVLTPATGRSGAAEFGGESF
                     SPDALEKFMPIVAEIQQACRQTGLELSAMVHEMGPAQIELNVAHGDALSRADQLFQLK
                     RLIKGCARKHGALASFMAKPLADLPGNGLHLHCSMEDETGRNIFALNRRRAPAVLQHF
                     IAGLQAHLPQAFALVAPNVNSYKRFVRDLSAPINLEWGYDNRTTGFRVPFSDARAGRV
                     ENRVAGADANPYLIIAATLACGLLGMRAAIKPTKPIADDAYGLKPALPEDLGEALRIL
                     EQSEGLIDLLGEAFVEVFVSVKRGELDHFGKTITQWEVGYLGSTL"
     gene            complement(4171..4923)
                     /locus_tag="AC16PP_14155"
     CDS             complement(4171..4923)
                     /locus_tag="AC16PP_14155"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011536407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR6482818.1"
                     /translation="MSRPVVLIPCDVKRYGEYPFHCVGEKYINAVAHGAGAMPLLMPA
                     WGRGAELRELTDLFDLDAILDRVHGVFLPGSTSNVHPHRYGGADLDMLHDEQRDAVVF
                     PLIERALSRGLPLLAVCRGLQELNVALGGTLHPRIHEVPGYLDHRENDELPRDAQYGL
                     VHAVDAVHGGMLADLVGAKRFLVNSLHAQGIAELGAGLRVEARAEDGLIEAVSMVGGW
                     VLGVQWHPEWHFTDDTASAKLFAGFGEALRAA"
     gene            5001..5819
                     /locus_tag="AC16PP_14160"
     CDS             5001..5819
                     /locus_tag="AC16PP_14160"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR6482819.1"
                     /translation="MSNLALISRDVAEILPAAGTDEFAPSVVAMLRDQVNIDEACMIV
                     YPRDESPRIAHRETQKPGDEPNLDTFIKGPFLLDPYYVAAAREGRFGFFPLSHLAPTG
                     FKTSEYYRTYYRFSGIFDECGYLTPIGDGGFANLSLARADGNQPFESSTLRCLADLAP
                     LVTALTQHHWRLLPANPSTEQISLRTQLESALESFGESVLTARERQIINSILHGHTSK
                     AIAQSLAISVETVKLHRKNAYRKLAIRNQSELFYTFIRALRDCSDEYQGGDPLR"
     gene            complement(5831..>6314)
                     /locus_tag="AC16PP_14165"
     CDS             complement(5831..>6314)
                     /locus_tag="AC16PP_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482820.1"
                     /translation="GVEGTANFLLGDYVDVYLGLSYLDTEATGLQSICGLEDAEGCEG
                     SSLFWAPEWTGSVVVNGNFPLEAGGALTGRVEGFWESERGGGFEGLKTTEIDSFFNLA
                     VRVGYESEHNWSANLYVENLTNEDTFDGLNNNGGVLPSHFFGPMRPRTFGVSFSYVWD
                     "
BASE COUNT         1184 a         2137 c         1918 g         1075 t
ORIGIN      
        1 ggcctgcagg cggaagtcac catcctggac cgttcggcgc aaaggctcca ctggctcagg
       61 cgccacttca acggccgggc tcgcgtgctg atggcagacg ggaacgcaat cggcaaggcg
      121 gtcgagacgg cggacctcgt cgtgggcgcc gttctcgtgc ccggggccgc agcgcccagg
      181 ctggttaccc gcgagatggt ggaagccatg gctaacggag cggccatcgt ggacatatcc
      241 atcgaccagg ggggatgttt cgagaccagc cgccccacga gccacgacca gccgacctac
      301 gtcgaagcgg gggtcgtcca ttactgcgtg accaacatgc cggggggcgt agcgcgaacg
      361 tccaccctcg ccctgagcaa ggtgaccctt ccgttcgtca tggaactggc aagccgtggc
      421 tgggaagacg cttttgcccg tgatcgccat ttcgcaaacg gcttgaacgt gcaccgtggc
      481 agcatccggc atccggaagt cgccaaggca ctggagcatg ccgtccctcc gatggctgcg
      541 taggcagccc ccgtcgacct tgagtccgcg ccgcggcctc agccgcggcg caaggcttcc
      601 tgacagatca ggtcgtgggc gatggtcgcc gcggcaatgg ccgtcagctc cgcgtgatcg
      661 tatgcaggcg cgacttccac cacgtccatc ccgaccagat tcaccggcgt cagcccgcga
      721 atgatgtgaa gggcctgagc cgacgagagg ccgcccgcca cgggcgtgcc ggtgccgggg
      781 gcaaaggcgg gatcgagaca gtcgatgtcg aatgtcagat acgccgggtg ttggccgacc
      841 acgctcagga cgcgctccag caccgcctgc ggaccgtgtt catgaaccca gggcgcgtcg
      901 cagaccacga agccatgatc ctgctgagaa aagctgcgga tgccgatctg cacggacctt
      961 tcgggcacga tcagaccctc ccgcagggcc cgcagaaaca tggagccgtg atcgatgcgg
     1021 ctgccgtcgt cctcccacgt atccacgtga gcatcgaaat gaatcagcga catgggccca
     1081 tgcttttcag cgtgcgcccg gagcagcggg taggtcacga aatgatcgcc cccgaacgac
     1141 agaagtttgc agcctcgagc aatgatgtcc cgggcatggg cttcgatggc cgcagcccca
     1201 tgctgggggt aaccggaatc gatgaagcag tcaccacagt cggcaacagc tagcgtggcg
     1261 aacggatcct ccccgtaagg aaacgcccgc ccggcaattc ccgccagctg cacgctggcc
     1321 gcgcggatgg cctgagggcc cagtcgcgcg cccggcctga aagtcgtcgc catgtcgaag
     1381 ggcaaaccgg tcaccaccag atctgcgccc tcgatgtccc tggtgtactt gcggcgaagg
     1441 aaggaaagct cgcccgcaaa ggtcatcccg gaacggtgcc ccagcaggtc ggatctcgtg
     1501 tgctcggagc gcgtcatctc atagacgatc cagaaaagcg tagtaaagca tacccgaaac
     1561 cagcgccggc cagcgcagat accgccctcc cgggaagggc aggtgcttga gcccggcaaa
     1621 cgtgtcgaat tcctcggcct ggcccataat cgcgtccgca accagcttac cgcccatgtt
     1681 ggccatcgcg atcccgtggc ccgagtatcc ctgagcccag tagacgttcg ctcccttgcg
     1741 cccgaactgc ggcatgcgcc gcatggtgat cgcaagcgtg ccgccccatc cgaagtcgat
     1801 ccgagcgctt tccagctgag gatagatctg gagcatccgc cgccgcacga acgccttgat
     1861 gtccttcgga aaccgcgacg agtagttctc gcccccgccg aagatcagcc gatggtccgg
     1921 cgagttgtgg aaatagttca ccacgaagcg ggtatcgacc acggcgagat cgtcccgatt
     1981 gatcgcgcgc cccatctcgg cccccagggg ttccgtggcc aggatgaagt tgttgatggg
     2041 catctgatag cgctcggcgc ccttctcgaa acgtcccaga taaccgttgc aggcgatgat
     2101 caggaatcgg gcgcgtacgt cgcctgatgc ggtgcgcacc aagaccctgt ccccctcctg
     2161 tcggtagccc agggccggcg tctgctcgtg aagcgttgcc ccgtgatcgc tggccgcacg
     2221 ggcgaggccg atggccaggt tcagcgggtg cacgtggccc gcgcgcaaat ccacgtagcc
     2281 gccgtacagg gtgtccgcgc cgagcatctt tgccacttcc tcgcggctga cgattctccg
     2341 gtccgagtat ccgtagtcac gctccagatg ctcggcgtac gaccacaacg ttttctcgta
     2401 acgctcgcgg tgcgctgccg tgagctcgcc gcttttcagg tcacagtcga tcccgaattc
     2461 ggcgatgcgg tcacgcacca ggtcattggc atcacaggcg atccgccaca gcttccgggc
     2521 cgcatcagcg cccagccagg actcgagctc cagctggtct tttcgctggc ccatgccgag
     2581 cacgccgccg ttgcgtccgg acgctcccga gccgacccga tgggcttcca gcaccaccac
     2641 gtcgtaattt ctgcggccga gctccagggc ggccgacagc ccggtgtagc caccgccgac
     2701 gatgcagacg tcggcttcgc aggatccatc gagaccgggg taggtccgca ttgtctttgc
     2761 gctgtggtaa taccaggact cgtcaaaccc gaccctggga tctcccccgc cccgagatcg
     2821 gctcacagcg tgctgcccag gtagccgact tcccactgag tgattgtctt gccgaaatgg
     2881 tcaagctcgc cacgcttgac cgacacgaag acctccacga acgcctcgcc caggagatcg
     2941 atgaggcctt cgctttgttc cagaatccgc agcgcttcac ccagatcttc cggaagcgcc
     3001 ggcttcagac cgtaggcatc gtcggcgatg ggcttggtcg gcttgatggc agcacgcata
     3061 cccagcagtc cgcaggcgag cgttgcagca atgatgaggt aagggttcgc gtccgcacct
     3121 gccacgcggt tctcgacgcg ccctgctcgg gcatcactga agggcacgcg gaagccggtg
     3181 gtccggttgt cgtaacccca ttccaggttg atgggcgccg acagatcccg cacgaagcgc
     3241 ttgtaggagt tcacgttggg ggcaaccaac gcgaacgcct ggggcagatg cgcctgcagt
     3301 cctgcaatga agtgctggag cacggcgggc gcccggcgac gattgagcgc aaagatattg
     3361 cgcccggtct cgtcctccat gctgcaatgc aggtgcagcc cgttccccgg caggtctgcc
     3421 agcggcttgg ccatgaagct tgccagggcg ccgtgcttgc gcgcgcagcc cttgatcagc
     3481 cgcttgagct gaaacagctg atccgcgcgg gagagcgcgt cgccgtgggc cacgttgagc
     3541 tcgatctggg cgggtcccat ctcgtggacc atcgccgaga gctcgagacc cgtctggcga
     3601 caggcttgct gaatctcggc cacgatgggc atgaacttct ccagcgcatc ggggctgaag
     3661 gactcgccgc cgaactcggc agcccccgac cttccggtcg ccggcgtcag caccaggtca
     3721 tggctggaca cagcttccag aagatagaac tcgacttcgg gagcgaccac ggggcgaagt
     3781 cccatggact cgtaagcatc cagcaccctc cgcagcacgt tacgcggatc gtagggcagg
     3841 cgttcacccg cctggtcgag gctttcgcag atcacctggc ccgtgtccgg cttccagggg
     3901 gtcgggacgt aggtcgacca atccggacgc aaaagcagat cgcgatcgtt ggggtcgtat
     3961 gtctccatgg cggccgcgta cccgcctgtg atggtctgga aaaagatcgc ttccgccagg
     4021 tggggcgggt gcgcttcgga atagctgatt ccacgcaggg tcttgccgcg gggcacgccg
     4081 ttcatgtccg acagcagcag ttccagttcg tccggcattt ccgatggctt gcccatccgc
     4141 tccctcctca gtcgaccagc cgggcagcaa tcacgctgcc cgcaaagctt cgccaaagcc
     4201 ggcgaacagc ttggcagacg cggtgtcatc cgtgaagtgc cactccggat gccactgcac
     4261 gcccagcacc cagccgccga ccatggacac tgcttcaatc aagccgtcct cggcccgtgc
     4321 ctccacgcgc agcccggccc ccagttcggc gataccctgg gcatgcagcg aattgacgag
     4381 aaaacgtttc gcgccgacca ggtctgcaag catgccgccg tgaaccgcat cgacggcatg
     4441 aaccagcccg tactgggcat cccgcggcag ctcgtcgttc tcccggtgat ccagatagcc
     4501 gggcacctcg tgaatccgcg gatgcagggt gccgccgaga gccacgttca gctcctgcaa
     4561 acccctgcat accgcgagca gcggcagacc gcgtgacagg gcgcgctcga tgagaggaaa
     4621 cacgacggcg tcgcgctgct cgtcatggag catatccagg tccgcgcccc cgtagcggtg
     4681 ggggtgaacg ttgctcgtag atcccggcag gaatacccca tggacccggt cgaggatcgc
     4741 gtccagatcg aacagatccg tcagctcgcg cagctctgcg ccccgacccc acgccggcat
     4801 gagcaggggc atcgcccccg caccgtgggc gaccgcattg atgtacttct cgcccacgca
     4861 gtggaacggg tactcaccat acctcttcac gtcgcaggga atgagcacga ccggcctgct
     4921 catcgcctcc ctccggctgc gtggcgagtc ggcctggctg gcgataagat gtggtgatcg
     4981 gcgacggatc gtgggcaaac atgtcgaatc tggcactcat cagtagagac gtcgctgaga
     5041 tcctgccggc agccgggacc gatgaattcg ccccgagcgt cgtcgcgatg ctcagggatc
     5101 aggtgaacat cgacgaggcc tgcatgatcg tctatccccg cgacgagtcc ccgcgcatcg
     5161 cccaccgcga aactcagaaa ccaggggacg aacccaatct cgacaccttc atcaaggggc
     5221 ccttcctgct cgatccgtat tacgtcgccg cagcccggga gggccgattc ggattctttc
     5281 cgctttccca tctcgccccc accgggttca agacaagcga gtactaccga acctactacc
     5341 ggttcagcgg catcttcgac gagtgcggct atctgacccc catcggggac ggcggctttg
     5401 cgaatctttc cctggctcgc gcagacggca accaaccgtt cgagtcctcc acactcagat
     5461 gcctggcaga tctcgcgcct ctggtcaccg cgctcactca gcatcattgg cgactgctac
     5521 ccgccaaccc gtctaccgag cagatcagcc tgcgaacgca gctggagtcg gcgctggaga
     5581 gctttggcga atcggttctg actgcacgcg agcgccagat catcaactcg atcctgcacg
     5641 gccacacgag caaggccatc gcccagtcgt tggccatctc cgtggagacc gtgaagctcc
     5701 accggaaaaa cgcctaccga aaacttgcca tccgcaacca atccgagctc ttctacacgt
     5761 tcatccgcgc gctgagggac tgcagcgacg agtaccaggg aggcgatccg ctgcgctagc
     5821 agcggctcac ctaatcccag acatagctga agctcacgcc gaaggtccgc ggccgcatgg
     5881 ggccgaagaa gtgggaaggc agaacgccac cgttgttgtt gagcccgtcg aaggtgtcct
     5941 cgttcgtcag gttctcgacg taaaggttgg ccgaccagtt gtgctccgat tcgtagccta
     6001 cgcgtaccgc cagattgaag aaggaatcga tctccgttgt cttcaacccc tcgaaaccgc
     6061 cgccgcgttc ggattcccag aacccttcca cgcggccggt gagggcgccc ccggcttcca
     6121 gcgggaagtt gccgttgacc accactgagc ccgtccactc cggcgcccag aacaacgagc
     6181 tgccctcgca gccttccgca tcctccagac cgcagatgct ctgcaggccg gtggcttccg
     6241 tatccaggta ggaaagcccc agataaacgt caacgtagtc gccgagcagg aaattcgccg
     6301 ttccttcgac gccc
//
LOCUS       DBNHKV010000434        11288 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1479810, whole genome shotgun sequence.
ACCESSION   DBNHKV010000434 DBNHKV010000000
VERSION     DBNHKV010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11288
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1479810"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="AC16PP_14170"
     CDS             complement(<1..1363)
                     /locus_tag="AC16PP_14170"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006720 - isoprenoid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6482821.1"
                     /translation="MSTVMRGANEPPTCHGGLENLDEEHDYWIDDIDGTIPADLTGTF
                     MRNGPGRQKIGGKPFGHWFDGDGMISQFTFTDSKAHFRNRYVRTPKYVEETEAQEILY
                     RGFGTQIPGGWTKNIFKWPANPANTSIIHHGGRLLALNEGGRPWELEPGSLETVGEFD
                     YDGALAGKAFSAHGKVHPQTGDYFNFGAGTTGTVRKSPCLNMYRINPQGRMAKTAQLP
                     LQTFPFCHDFALAGRHAVYFVNSIVFGGMGGFFLGRHSMSDGVRFDDDIPMRIIVVDL
                     DTLEVVREFETDPGAIIHFGNAFESGDEVVVDAMFQDNFEVNATLADVFNPSGRFGGG
                     YYNRYRLNLADGTMSCARVSECEAEFPVFNPAKAGQPHSVTWTACSVENGANSFFNGF
                     QRVTFDGDADVVTLPAGCYGSEPMFAAAEGATREDDGYLLEVVYDGYSHRSELQVYRA
                     DDVN"
     gene            complement(1365..2354)
                     /locus_tag="AC16PP_14175"
     CDS             complement(1365..2354)
                     /locus_tag="AC16PP_14175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6482822.1"
                     /translation="MASRPEVEVEPGNFELVEEPVSGLEPGTLLLKTRYLTVNPPMRM
                     ALVSGGIAGKPIPIGRTMYGSGLAEVVESRNPAFAAGDLVMGDLGWQEYVVSDGARRR
                     GVRKVAVPERLSPTTLMHVLGGSGHTAYFGMIEFCKPRVGDTLVVSTAAGSVGALVCQ
                     LGRLQGCRVIGIAGSDEKCEWVVDELGAEACINYRTEDVAARLAETCPNGIDVYFDNV
                     GGETLDAVLGQIAMGARVVLCGGTSQYNNDLDWYGPKNYFNLVYKQAEMSGYFVSNFA
                     HRFDESLARLTTLVRDGRLKYREDVLEGIESVPSALIRILRGENFGIPLIKVS"
     gene            complement(2441..2893)
                     /locus_tag="AC16PP_14180"
     CDS             complement(2441..2893)
                     /locus_tag="AC16PP_14180"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR6482823.1"
                     /translation="MSNHTACVEWTMGEGDFVSGRYSREHRWTFDGGAVVQASPTPAV
                     VPEPFSNPACVDPEEAFVAAVASCHMLTFLHIAGRAGFSVIGYRDEAVGTMTKNAQGA
                     YWISEITLNPAIEYAGNAPTEAELDDLHDQAHEHCFIANSIRTEVLVG"
     gene            complement(2957..3646)
                     /locus_tag="AC16PP_14185"
     CDS             complement(2957..3646)
                     /locus_tag="AC16PP_14185"
                     /inference="COORDINATES: protein motif:HMM:NF016705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOUL family heme-binding protein"
                     /protein_id="HIR6482824.1"
                     /translation="MLKVLMIAAGVLVAVVVAGIVAWWYWGTTLEQPEYAVISKGDGY
                     EVRRYEGYLVAQTRVSGEFDQAGTRAFPVLAGYIFGNNVERREMAMTTPVVSYDADTS
                     SGSTMAMTAPVFSEPAGAEDEASVDPSQEKLWTYQFVMERKYSEASLPAPVDPRVEIK
                     RIPPRLVAARRFSGRWLEENVKAAETALLSSVRAGGLEPVGQVVLARYNDPFTPWFLR
                     RNEVLVEIAGD"
     gene            3847..4611
                     /locus_tag="AC16PP_14190"
     CDS             3847..4611
                     /locus_tag="AC16PP_14190"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6482825.1"
                     /translation="MSNPTQPLKLYGVPFSQPVRAVMWLMLHKRMPFEMVLTNPGTSG
                     ENGSRHPDYLAKNPGGTIPTIEEPDTGFTLGEAHAILCYLCNKYGWSDMYPAEPQERA
                     RVDWYLHYHHRNVREASTGLVAPKIRKDLNIPETVQAAAQATLTRALQAIETGWLGKS
                     AFLAGDAVTIADFAAYVEIGQLQPGFTNVYDFNPFPNVRRWMDSLKTFDSHDDVHVVL
                     AELGDISAEPPSMEAIKNANISALRTLKARLAEMSG"
     gene            4646..5074
                     /locus_tag="AC16PP_14195"
     CDS             4646..5074
                     /locus_tag="AC16PP_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007947151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR6482826.1"
                     /translation="MSETHKGACFCGAVQIEVTGEPNMMGYCHCTDCAAWAGAPVNAF
                     SLWPPENVQVTAGEEKLGTYAKTEGSQRKFCTQCGGHVFTDHPGMGLIDVYLNVVPGK
                     AHTGAMHVFYGEKTMSVPDGLPKYRDLPKEFGGSGETIAE"
     gene            complement(5142..5828)
                     /locus_tag="AC16PP_14200"
     CDS             complement(5142..5828)
                     /locus_tag="AC16PP_14200"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6482827.1"
                     /translation="MSDDLLDNPAFAALQHAHAGLGRASELAAVYDPLVSPLAGLSEA
                     TPEALLDLAGLVPSGAGVGVLSTQPRLPESAGWRLLRVMPLHQMVCETPPGQPEPFDE
                     LTPSDAPEMVALAELTEPGPFLERTIEMGRYVGVRSGGRLVAMAGERMKLPGCTEVSG
                     VCTHPDARGRGLALRMVLNVLAECFARGDRAFLHVVAGSPSETTAIGIYERLGFEHRR
                     PIYAHVLLRE"
     gene            complement(5974..6897)
                     /locus_tag="AC16PP_14205"
     CDS             complement(5974..6897)
                     /locus_tag="AC16PP_14205"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6482828.1"
                     /translation="MHRALGVDQLGCTLFVLVWAFIGANPWEENHMAFEAIRYEKTDG
                     VATITKNNPPQYGMTLAVLTEMRAALIDANNDADVCVVVITAGGEGFHMGAVVFGEAR
                     SDWKFSPLEFREVIQVGHDLFRLIETLEKPVVGVAKAGAVGGGFENLHACDFVIASEA
                     ARFSQPEATLGMICGWGGTQRVPRMVGWRKAKEILMEGVELTGREAAEIGLITRAVPL
                     DEVDEAVSALCERLKICGPVALGYTKLAMNKVWETDYRSGLDYEVEASGMVNSAGEFT
                     AEVFEDFLAGRQPKFAKRQRLTAGREWRGTD"
     gene            complement(6916..7290)
                     /locus_tag="AC16PP_14210"
     CDS             complement(6916..7290)
                     /locus_tag="AC16PP_14210"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6482829.1"
                     /translation="MPDLAIGYVNVFVSDFDTALAFYRDKLGLEVAMADEEFGYASFD
                     TKGVGFAIAAVGQDAEPAFAPGRHTGIGWGVESLDDAYETLRSTGVEFTAPPERQPWG
                     GYMALFEDPDGNVFYLDELREH"
     gene            complement(7411..8265)
                     /locus_tag="AC16PP_14215"
     CDS             complement(7411..8265)
                     /locus_tag="AC16PP_14215"
                     /inference="COORDINATES: protein motif:HMM:NF025679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HIR6482830.1"
                     /translation="MTVGLTDAPVDGVMAVHVEFRGITLKPQSGPAIDFALSEKVQLL
                     DLQNGSVFPLLDAQTVDAGRYNWIELHTNAEIDGILDSYVVESKSNGMVELEVPSGTV
                     RLVSGFVVTAGKSNAVLLDWDVRKGLTDPEGLVSPSPDAKDAYKLTPALRLIDMTQYG
                     SLTGSVADALIMAESCTSNAEGDGNLVYVYRNHDTTPDDLGSFGEPVTTAPVRSNPEK
                     AGAYTYTVHYLDPGDYTVAFTCQGLDDDPATDAAEGAAVPDIEFSGQVNASVAEGEGT
                     TNEAPPID"
     gene            8678..9187
                     /locus_tag="AC16PP_14220"
     CDS             8678..9187
                     /locus_tag="AC16PP_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482831.1"
                     /translation="MAGNLGLIAGLILVGVQITDSNRIASAELFGSSVGSVISINTEQ
                     LGDSPGEAMSRVLYDPAAAETSDLYIADRIYDALLRQLVRVHVFDAMGLYGDDELVSP
                     AGFVGLHFEFFACPYGLAWLDQQIELFDTRGNEELAILTSLKMLRELAGARPLVLVCS
                     ATDKRTSAR"
     gene            9252..10481
                     /locus_tag="AC16PP_14225"
     CDS             9252..10481
                     /locus_tag="AC16PP_14225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450 family protein"
                     /protein_id="HIR6482832.1"
                     /translation="MIDLNRPVNLASPEFAKDKYEWYERIREERPVFQTKISVLTVYT
                     VSRYEDCTNVLKDPNVLRNRTTATGGSRFPFPMPKSVQPLVQSMIQEDDPNHRRLREL
                     VRRAFRPQAIEQLESQIDAYSHELLDGLARRDSFDLQADYARPIPIRMIGDMMGLSRE
                     AVPQFQKAIRVLSEGFNGLKVLRTLFLDLPGAVRFVRALLDEKRSMPGDDVLTGLIEA
                     EDDGDRLTDDELVAMVFLLIVGGFETTLHLITNGVVTLLEHPEQLQRLKEDPGLIDSA
                     VEEILRHRGPVHGTKPGYAREDITLHGMTIPKGKPIMPLLAAANHDPRVFEKPQTFDI
                     ARSPNRHLGFGHGVHFCLGAHLARAEARVALRNLFERFPDLQLAVDPASLKLQAMPGW
                     HRYDGLPVIAGKMRSAA"
     gene            complement(10488..>11288)
                     /locus_tag="AC16PP_14230"
     CDS             complement(10488..>11288)
                     /locus_tag="AC16PP_14230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR6482833.1"
                     /translation="GIEVVRDVPDVGARMREHLGFSMPHKLKGTKGLNHRLRGLGLVG
                     SALQYVATRGGPLATGPFEVGAFVRAVPGANRPDTQLYLGAFNYARSNDNFPVQLASP
                     DSKPGMTIYGQLLNLTSEGTVRLRSADPDDPPVIVPNWLSTDYDRAAAVAMVRYMRRY
                     VRQPALARYVGDELIPGPTCQTDEEILNAVRRLSTSGLHGVATCRMGRDEGAVVDGRL
                     RVRGVEGLRVADCSVMPSLVSGNTNAPAMALGWRASDLIEEDAAGSRR"
BASE COUNT         2107 a         3780 c         3420 g         1981 t
ORIGIN      
        1 cattgacgtc gtcggcccgg tagacctgca gctcgctgcg gtgtgagtag ccgtcgtaaa
       61 ccacttccag aaggtagccg tcgtcttcgc gggtcgcgcc ctctgcagcc gcgaacatgg
      121 gctccgagcc gtagcagccg gccggcaggg tgacgacgtc tgcatcgcca tcgaaggtca
      181 ctctctgaaa accgttgaag aagctgttgg cgccgttttc caccgagcag gccgtccagg
      241 tcaccgagtg cggctgcccg gctttcgccg ggttgaagac ggggaactcg gcctcgcatt
      301 cactcacccg tgcgcagctc atggttccat cggccaggtt caggcggtag cggttgtagt
      361 acccgccgcc gaaccttccc gacgggttga acacatctgc cagcgtcgcg ttgacctcga
      421 agttatcctg gaacatggcg tccacgacca cctcgtcacc gctttcgaac gcgttgccga
      481 agtgaatgat cgcgcccgga tccgtctcga actcgcgcac cacctccagg gtgtcgagat
      541 caacgacgat gatgcgcatg gggatgtcgt catcgaaccg cacgccgtcc gacatgctgt
      601 gtcttcccag gaagaaaccg cccatgccgc caaagacgat ggagttgacg aagtacacgg
      661 cgtggcgccc ggccagggcg aaatcgtggc agaacggaaa cgtctgcagc ggaagctgcg
      721 ccgtctttgc catgcgaccc tggggattga tgcggtacat attcaggcac ggtgatttcc
      781 tcacggtgcc cgtggtcccg gcgccgaagt tgaagtagtc gcccgtctgc ggatgcacct
      841 tgccgtgggc gctgaacgcc ttgcccgcga gcgcgccgtc gtagtcgaac tcgcccaccg
      901 tctctaaaga gcccggctcg agctcccacg ggcgaccgcc ctcgttcagc gccagcagcc
      961 ggccaccgtg gtggatgatg ctggtgttcg cggggttggc cggccacttg aagatgttct
     1021 tggtccagcc tccggggatc tgcgtgccga atccccgata gaggatctcc tgcgcttcgg
     1081 tctcctcgac gtacttgggc gttctgacgt atcggttgcg gaagtgggct ttggaatcgg
     1141 taaacgtgaa ctggctgatc atgccgtcac cgtcgaacca gtgaccaaag ggcttgccgc
     1201 cgatcttctg acgccccgga ccattgcgca tgaacgtgcc ggtgaggtcc gcaggaatcg
     1261 ttccgtcgat gtcgtctatc cagtagtcgt gctcctcgtc cagattctcc aggccgccgt
     1321 gacaggtagg cggctcgttg gcgcctcgca ttaccgtgct catgtcatga aaccttaatc
     1381 agtgggatgc cgaaattctc gccgcgcaga atccggatga gcgccgacgg aacgctctcg
     1441 atgccctcga gaacgtcctc gcgatacttc agacgcccgt cgcggacgag ggtggtcagc
     1501 cgggcgagag actcatcgaa acggtgcgcg aagttggaga cgaagtagcc ggacatctcc
     1561 gcctgcttgt aaaccaggtt gaaatagttc ttggggccat accagtcgag gtcgttgttg
     1621 tactgcgacg tgccgccaca gaggaccacg cgggcgccca tggcgatctg cccgagcacc
     1681 gcatcgagcg tctcgccccc gacgttgtcg aagtagacgt ctatgccgtt cgggcaggtt
     1741 tcggccagac gcgcggcaac gtcttccgtg cggtagttga tgcaggcctc ggcaccgagc
     1801 tcgtcgacca cccactcgca cttctcgtcg ctgccggcaa tgccgatcac ccgacagccc
     1861 tgcaggcggc cgagctggca gacgagcgcg cccacgctgc ccgccgcggt tgagaccact
     1921 aaggtgtcgc ccacccgggg tttgcagaac tcgatcatcc cgaagtaggc cgtgtgcccg
     1981 cttcccccga gcacgtgcat gagggtagta ggtgacagcc gttcaggcac cgcgaccttc
     2041 cgcaccccac gccggcgcgc gccgtcagag accacgtact cctgccagcc gaggtctccc
     2101 atgaccaggt ctcccgcggc aaaggccggg ttgcgagact ccaccacctc ggccaggccg
     2161 ctgccgtaca tggtccggcc aatggggatg ggcttcccgg ctataccgcc ggaaaccaac
     2221 gccatgcgca tgggcgggtt cacggtgagg tatcgggtct tgagcagcag ggtgccgggc
     2281 tccaacccag aaaccggttc ctccacgagt tcgaagttgc ccggttccac ctcgacttcc
     2341 ggccgagacg ccaccaccca ctttctgttg atcaggccgc tgatgttcta ccccctctac
     2401 tcgtccgtgc ccatcgtacc ggtagccgtc taatcacacg tcagccgacg agcacctcgg
     2461 tgcggatgga gttcgctatg aagcaatgct cgtgcgcctg atcgtgcagg tcgtccagtt
     2521 ccgcttccgt aggcgcgttg ccggcgtatt cgatcgccgg attgagcgtg atctcgctga
     2581 tccagtacgc accctgggcg ttcttggtca tggtgcccac cgcctcgtcg cgatagccga
     2641 tcaccgaaaa tcccgcacgg ccggcgatgt gcaggaaggt aagcatgtgg cagctggcca
     2701 ccgccgcgac gaaggcctct tccggatcta cgcaggcagg attcgaaaac ggctccggca
     2761 ccaccgccgg cgtgggcgat gcctgaacca ccgcaccgcc atcaaacgtc cagcggtgct
     2821 cgcgggaata gcggccgctg acgaagtccc cttcccccat ggtccactcg acgcaggctg
     2881 tgtggtttga catgacgttt ctccaaccag aggcaacaat cctacgccga gacagacccc
     2941 acgaccatgg cggctgtcaa tcgccagcga tctccacgag cacctcgttg cgtcgcagaa
     3001 accacggcgt gaaggggtcg ttatagcggg cgaggaccac ttgccctacg ggctcgaggc
     3061 cgccggcgcg caccgaactc agcagcgccg tctccgcagc cttcacattc tcctcgagcc
     3121 atcggcccga gaagcggcgc gccgccacca accgcggcgg aatccgcttg atctcaacgc
     3181 gcggatccac aggcgcgggc agtgacgcct cggagtactt gcgttccatc acgaactggt
     3241 acgtccacag cttctcttgc gatgggtcaa ccgatgcctc gtcttctgct ccggcaggct
     3301 cgctgaaaac gggcgcggtc atggccattg tgctgcctga agacgtgtcg gcatcgtagc
     3361 tcaccaccgg cgtggtcatg gccatctccc gacgctccac gttgttgccg aagatgtacc
     3421 ccgctagcac ggggaacgcc cgggtacctg cctggtcgaa ctccccggac acccgcgtct
     3481 gcgcgacgag gtagccttcg tagcgacgaa cttcgtagcc atcacctttc gagatcaccg
     3541 cgtattcggg ctgttcaaga gtcgtgcccc agtaccacca ggcaacgatc ccggccacga
     3601 cgacggcaac gagcacgcct gctgcaatca tcaggacctt caacatgatg ggagcttcgt
     3661 tcgggcgacg agcatggcgg tcatggtctt tcctttctcc tcgaacctgt attcaaccat
     3721 gctcgcgaga cggtgaaatg cacgtgaatc ccccgcgccg gaatgcgacg ggactgcggt
     3781 gcagcctcgg gagcttatca ccggacagaa ggcatgatag cttggccgtc cgatcaggga
     3841 ggaatgatga gcaaccccac ccaaccactg aaactctacg gcgtgccgtt ctctcaaccc
     3901 gtccgcgccg tcatgtggct gatgcttcac aaacgtatgc cgttcgaaat ggtactcacc
     3961 aaccccggca catcgggcga gaacggcagt cgtcacccgg actatctggc caagaatccg
     4021 ggcggcacca tcccgaccat cgaggagccc gataccggct tcaccctggg cgaagcgcac
     4081 gccatcctct gctatctgtg caacaaatac ggctggtcgg atatgtatcc agccgagcct
     4141 caggagcgcg cccgcgtgga ctggtacctg cactatcacc atcgcaacgt cagagaggca
     4201 tcgaccgggt tggtggcgcc gaagatccgg aaagatctga acattccgga gaccgtccag
     4261 gcggccgccc aggccaccct gacccgggcg ctgcaggcca tcgagaccgg ctggctcgga
     4321 aagtccgcat ttctcgccgg cgatgcggtc accatcgccg acttcgccgc ctacgtggag
     4381 atcggccaac tgcagcccgg cttcaccaac gtctatgatt tcaacccgtt tccaaacgtt
     4441 cgccgctgga tggactcgct caaaaccttc gacagtcacg acgacgtcca tgtcgtgctc
     4501 gccgagctgg gcgacatcag cgccgagccg ccttccatgg aggcaatcaa gaacgccaac
     4561 atctccgctc tgagaacgct caaggcgcgc ctggcggaga tgagcggatg acccggcaat
     4621 cctgaacacg agcaaaggaa taatcatgag cgaaactcac aagggcgcct gcttctgcgg
     4681 agcggtccag atcgaggtca ccggagagcc caacatgatg ggttactgcc actgcacgga
     4741 ttgcgcggcc tgggccgggg ccccggtgaa cgcgttcagc ctgtggccgc cggaaaacgt
     4801 ccaggtgacg gcgggcgagg agaagctggg cacctatgcc aaaaccgaag gcagccagcg
     4861 caagttctgc acgcaatgcg gcggccacgt gttcaccgac catcccggga tgggactaat
     4921 cgacgtttat ctcaacgtcg tgcccggtaa ggcccacacg ggcgccatgc acgtcttcta
     4981 cggcgagaag acgatgtcgg taccggacgg tttgccgaaa taccgggatc ttccaaaaga
     5041 gttcggcggt tccggcgaaa ccatcgccga gtaggaccgc cgcgagcggt gagcggagga
     5101 cccggggtcc ggtctgcacg ggccgccgat tgagacacgg tttactcgcg cagcagaacg
     5161 tgggcgtaga tcggcctgcg gtgttcgaag ccgagcctct cgtagattcc gatggcggtg
     5221 gtctcgctcg ggctgccggc caccacgtgc agaaacgccc gatcgccacg ggcaaagcat
     5281 tcggccagca cgttcagaac cattcgcaag gccagccccc gaccgcgcgc gtcgggatgg
     5341 gtacacacgc cgctgacctc cgtgcagccg ggcagtttca tgcgctcgcc agccatggcg
     5401 accagccggc cgccgctgcg cacgcccaca tagcgcccca tctcgatggt ccgctccaga
     5461 aacggcccgg gctcggtcag ctcggcaagg gcaaccatct ccggagcgtc ggacggcgtc
     5521 agctcgtcga agggctccgg ctgccccggc ggcgtctcgc agaccatctg gtgcagcggc
     5581 atgacccgca gcagccgcca gcccgcggat tcggggagcc ggggctgggt cgaaagaacg
     5641 ccgacgccgg cgcccgatgg caccagaccg gcaaggtcga gcagggcttc aggcgtcgct
     5701 tcggaaaggc cggcgagcgg cgataccagc gggtcgtaga cggcagccag ctcgcttgcc
     5761 cgaccaagac cagcgtgagc gtgttgcagc gccgcgaaag ccggattgtc gagcagatcg
     5821 tctgacatca gcaccagctc cgattcggtg cggcccctct gccacgacgt gcaaagcggc
     5881 ggctcacttt ctgcattctt gcccatcgag ccgaagatag ttcgagccag ggggcgtgtc
     5941 taacggccct cgcccggcaa taggtgtgcg ctgctagtca gtcccccgcc attcccgtcc
     6001 agcggtaagc cgctggcgtt ttgcgaattt cggctgccgc ccggcgagaa aatcttcgaa
     6061 cacctcggca gtgaactccc cggcgctgtt gaccataccg gacgcctcta cctcgtagtc
     6121 caggcccgag cggtagtccg tttcccagac cttgttcatc gcaagcttgg tgtagccgag
     6181 cgccaccgga ccgcagatct tcaacctttc acagagggcg gagacggcct catccacctc
     6241 gtcgagcgga accgcacggg tgatcaagcc gatctccgcg gcttccctgc cggtcagctc
     6301 cacgccttcc atgaggatct ccttcgcctt gcgccagccc accattctcg ggaccctctg
     6361 ggtaccgccc cagccgcaga tcattccgag cgtcgcctcc ggctggctga agcgcgcggc
     6421 ctcggaggcg atgacgaaat cgcaagcgtg gagattttcg aagcctccgc ctacagcccc
     6481 cgccttggcc acgcccacga cgggtttctc cagcgtctcg atcaggcgga aaagatcatg
     6541 tcccacctgg atgacctcgc gaaactccag gggggagaac ttccagtcgc tgcgcgcctc
     6601 gccgaaaacc acggccccca tgtgaaagcc ctcgccccct gcagtgatga ccacgacaca
     6661 aacgtcagcg tcgttgttgg catcgatgag cgcagcccgc atctccgtca gcacggcaag
     6721 cgtcatgccg tactgcggcg ggttgttctt ggtgatggtg gcaacgccgt ccgtcttttc
     6781 gtagcgaatg gcctcgaacg ccatatggtt ttcctcccag gggtttgcgc ctataaacgc
     6841 ccagaccagc acgaagagcg tgcagccgag ctggtcgacc cccagcgcgc gatgcaacgc
     6901 aaggagagcc ggccctcaat gctcccgaag ctcgtccaga tagaagacat tgccgtcggg
     6961 atcttcgaac aacgccatgt aaccgcccca cggttgtcgc tcggggggag ccgtgaactc
     7021 gacccctgtg ctgcgaagcg tttcgtaggc atcgtccaga ctttcaactc cccagccgat
     7081 cccggtatgt cgccccggag caaaggccgg ttcagcgtcc tggccaaccg cggcaatggc
     7141 gaacccgacg cccttggtat cgaaggacgc gtagccgaac tcctcatcgg ccatcgcgac
     7201 ctcgagaccc agcttgtccc gatagaaggc cagcgccgta tcgaaatcac tgacaaagac
     7261 gttcacgtac cctatggcca gatcaggcat tctgctctcc cgaacgccac gacaagacca
     7321 tacgatacca ccactgcggg gtgcgcgagc gcttacatga ccaatgccgg ggcgcgatcg
     7381 gccacacgct aggccgctgc ggtcaaacga tcaatcgata gggggcgctt cgttggtcgt
     7441 cccttcgccc tcagcaaccg acgcgttgac ctgaccgctg aattcgatgt ccggcacggc
     7501 ggcgccttca gccgcgtccg tggcgggatc gtcatcgagt ccctgacagg taaaggcaac
     7561 cgtgtagtcg ccaggatcca ggtagtgcac cgtgtaggta tatgcacccg ccttctccgg
     7621 gttggaccgg accggcgccg tggtcacggg ctcgccgaag ctgccgagat catcgggcgt
     7681 cgtgtcgtga tttcggtaaa catacaccag gttgccgtcg ccttcggcgt tgctcgtgca
     7741 ggactcagcc atgatcaaag catccgccac gctgccggtc aggctgccgt actgggtcat
     7801 gtcaatcagt cgcagcgccg gggtgagctt gtacgcatcc ttggcgtctg gactcgggct
     7861 caccagcccc tccggatcgg tcagcccttt gcggacgtcc cagtcgagaa gcaccgcgtt
     7921 gctcttgccg gcggtcacca caaaaccgct caccagacgt accgtgccgc tcggcacctc
     7981 cagctcgacc atgccgttgg atttgctctc caccacgtac gaatccagaa tcccgtcgat
     8041 ctccgcgttg gtgtgcagtt cgatccagtt gtagcggccc gcgtcgacgg tctgggcatc
     8101 cagcaacgga aacacgctgc cgttctgcag gtcgaggagc tgaaccttct cggagagcgc
     8161 aaagtcgatg gcaggcccgc tctggggctt gagcgtgatg ccacggaatt caacgtgaac
     8221 cgccatcacg ccgtcgacgg gggcatccgt caggccgacg gtcaacgtgc cggtggatgg
     8281 accctcatcg gatccgctgg atcccccaca tcccaacatt gaccaggtca acgccgcaaa
     8341 gacaatcgaa cggtacgcag acataactcc ttctcctgtt gaccttcgac cacgcggctg
     8401 tttccacgcg tgaacgagac ctaagcaggt atgacgcatc gggtcgccga tcggctgcga
     8461 cgcatgcgtc gatctgccaa atggttcacc cccgaggcgt tagcggcttc acgctcgggt
     8521 ggctgtaacc gagaaagtca tgcggtgctg ataagcggtg cagagccgcg caagccaggg
     8581 tcgaaacgag aaacggccga cttctgctaa cctcgatcca atcacaatct ccgggagaac
     8641 cattgtggcg cgcgcaaagt tgttggagct tttgcaggtg gcaggcaatc tggggctgat
     8701 cgccgggctg attctggtcg gcgttcagat caccgactcg aaccgcatcg cctcagcgga
     8761 gctcttcggt agcagcgtcg gctccgtcat ctcaatcaac accgagcagc tcggcgactc
     8821 acctggtgag gcgatgtcac gcgtgctgta cgaccctgct gccgcggaaa cctcggacct
     8881 ctacatcgcc gaccgaattt acgacgcgct gctgcgccag ctggtccgtg tgcacgtatt
     8941 cgacgccatg gggctttacg gtgacgacga gctcgtcagc cccgcggggt tcgtaggcct
     9001 tcacttcgag ttcttcgcct gtccctatgg cctcgcctgg ctcgaccagc aaatcgagct
     9061 gtttgacacc cgcggcaacg aggagctggc aattctcacg agcctgaaga tgctgcggga
     9121 gctcgcgggc gcacgtcctt tggtattggt atgctctgca acagataaac gaacgtctgc
     9181 ccggtaaagg ggcaacagcg gtccgataga tccggacgag cacccgctgc gaggcgccag
     9241 gagtccagcc catgatcgac ctgaaccgcc cggtgaatct ggcttcgccg gagttcgcga
     9301 aggacaagta cgaatggtac gagcgcatcc gcgaggagcg cccggtgttc cagaccaaga
     9361 tcagcgtgct taccgtctac accgtttccc gctacgagga ctgcacgaac gtcctcaagg
     9421 acccgaacgt tctgcgcaac cgcaccacgg ccaccggagg aagccgcttc cccttcccca
     9481 tgccgaagtc cgtccagccg ctcgtgcaga gcatgatcca ggaagacgac ccgaatcacc
     9541 ggcgcctgcg cgagctggtc agacgggcct tccggcccca agccatcgag cagctggaga
     9601 gccagatcga cgcctactcc cacgaactgc tcgacgggct ggcgcgcagg gacagcttcg
     9661 atcttcaggc cgactacgcg cgaccgatcc ccatccgcat gatcggcgac atgatggggc
     9721 tttcccgcga ggcggtaccg caatttcaga aggcgatccg agtgctctcg gagggcttca
     9781 acgggctgaa ggtgctgcga acgctgtttc tggatctgcc cggcgccgtt cgtttcgtcc
     9841 gcgccctgct cgacgagaaa cgctcgatgc cgggcgacga cgtcctcacc gggctcatcg
     9901 aagccgagga cgatggcgac cggctcaccg acgacgagct ggtggccatg gtatttctgt
     9961 tgatcgtcgg tgggttcgag acgacgctgc atctcatcac caacggggtc gtaacgctcc
    10021 tcgagcaccc cgagcagctg cagcggctga aggaagatcc cggcctgatc gattcggcgg
    10081 tggaggagat cctccgacac cggggtcccg tgcacggcac caagccgggc tacgcccggg
    10141 aggacatcac gcttcatggc atgacgattc cgaaggggaa gcccatcatg ccgctgcttg
    10201 ccgccgccaa tcacgaccca cgggtgttcg agaaaccaca gacattcgac atcgcccgct
    10261 cacccaatcg tcacctcggc ttcggccatg gcgtgcactt ctgcctgggc gcacacctcg
    10321 cccgggccga agcgcgggtg gcgctgcgca acctattcga gcgatttccc gacctgcagc
    10381 tggccgtgga tccggcgtcc ctcaagctgc aggccatgcc gggctggcac cgctacgacg
    10441 ggctgcccgt gattgccggg aaaatgcgct cagccgccta gctgcgatca gcgacgcgag
    10501 cctgccgcgt cctcttcgat caggtcgctt gcccgccaac ccagcgccat ggccggggca
    10561 ttggtgttgc ccgagaccag ggagggcatc acagagcaat cggcgacgcg caaaccttcg
    10621 acgcccctga cccgcaaccg gccgtccacc accgcgcctt cgtcccggcc catccggcag
    10681 gtggccacac cgtgcaggcc gctggtggaa agccgccgca cggcgttgag gatctcctcg
    10741 tccgtctggc aggtcggccc ggggatcagc tcgtcgccga cgtacctggc cagcgctggc
    10801 tggcgcacgt agcggcgcat gtaacggacc atggcgaccg ccgccgcgcg atcgtaatcc
    10861 gtgctgagcc agttgggcac gatcaccggc gggtcgtcag gatcggcgga gcgcagacgc
    10921 accgtgcctt cgcttgtcag attcaggagc tggccgtaga tggtcatgcc cggcttgcta
    10981 tcgggcgacg ccagctgaac tggaaagttg tcgttgctgc gcgcgtagtt gaaggcgccg
    11041 aggtagagct gcgtgtccgg ccggttggcg ccgggcacgg cgcggacgaa ggccccgacc
    11101 tcgaagggtc cggtggcgag cgggccgccg cgggtcgcca cgtactgcag cgcgctgccc
    11161 accagaccca ggccgcgcag ccgatggttc aggcccttgg ttcccttaag cttgtgcggc
    11221 atcgaaaaac ccagatgctc gcgcatccgc gcgcccacgt cgggaacgtc gcgcaccact
    11281 tcgatgcc
//
LOCUS       DBNHKV010000435        10725 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_59966, whole genome shotgun sequence.
ACCESSION   DBNHKV010000435 DBNHKV010000000
VERSION     DBNHKV010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10725)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10725)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10725
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_59966"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="AC16PP_14235"
     CDS             <1..628
                     /locus_tag="AC16PP_14235"
                     /inference="COORDINATES: protein motif:HMM:NF024591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR6482834.1"
                     /translation="ATEGLGFTGCTTEEWLAHKGTVGRVLLGELHILDEDGDPSPKGE
                     PGEVWFKTASAFEYFNDPEKTAQATSADGSMTTVGDVGYVDDDGYLYLTDRATFMIIS
                     GGVNVYPQECENLLITHPKVADAAVFGVPNVDLGEEVKAVVQVMPDVEPGEALEAELI
                     AFCHEHLARQKCPRSVDFEEQLPRLPTGKLYKRHLRDRYWGDKKSRIV"
     gene            complement(656..1864)
                     /locus_tag="AC16PP_14240"
     CDS             complement(656..1864)
                     /locus_tag="AC16PP_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482835.1"
                     /translation="MLNVETLRVTLVAAMVLLAAQPAHADGTYGADFRAHLLPEAGTA
                     HASITITQEAGELKRLDLAAPAATYSAFSGPGSIRREEDRLVWTVPEDGGTLSYDVLI
                     DHRRNGAYDARMTPDWAIARLDDLFPRARTRSESGAQSRSSVVLAGPAGWRFETRYGP
                     SHEQVAVVDEGRRFDRPTGWLVAGRLGIRRDTIAGRTVSVAAPVGQGFRRVDVLAFLR
                     WTLPDLVAVFPGMPDRLLVVGAPEGMWRGGLSGPESLYLHVGRPLISENGTSTLLHEL
                     VHIATTAPKSAGDDWIMEGLAEYYSLETLRRSGGISEERFASALGSLAAWAQRDGGRL
                     ADPSTGAHTARAALTFHELAAELEDAGSSLDAVVGELVAGGSLDRGSLLALLEPKLTE
                     PSGILAELPR"
     gene            2026..2793
                     /locus_tag="AC16PP_14245"
     CDS             2026..2793
                     /locus_tag="AC16PP_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6482836.1"
                     /translation="MIDVYYWPTPNGWKVTIMLEECGLPYRILPVDIGGGDQFTPDFL
                     RVSPNNRMPAIVDHEPLGGGEPLSIFESGAILEYLADKSGKFLSREPAGRYRTLQWVH
                     WQMANLGPMMGNANHFKNYGKNIAEDPKQLEYGTRRFVGEVDRLSGVMDAQLRVNRYL
                     AGSEYTIADIITWPWAMLLGRLIDEAVWETFPNLKRWVDEVGERPAVEKGRKVERDLG
                     RQERTEEQEKARRELLFNQTNEKVRVAREAAAKAAAG"
     gene            2921..3301
                     /locus_tag="AC16PP_14250"
     CDS             2921..3301
                     /locus_tag="AC16PP_14250"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6482837.1"
                     /translation="MAIRELVDSYNDAVMRFDGDAWAANWRDDATWSLPGAGPIKGKE
                     NFFPMWQQAMSAFSFVGFFASAGPIVVDGDTAHATWYQQEFLHQKDGVKRNVTGRYED
                     DYVKVDGRWYFQQRIYEVMNAEES"
     gene            complement(3307..4467)
                     /locus_tag="AC16PP_14255"
     CDS             complement(3307..4467)
                     /locus_tag="AC16PP_14255"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6482838.1"
                     /translation="MAEANQDTSPDYGPHEEAMGAYLREGEARALALGNRGPIRFGAD
                     GRLAADILDAYSRLGFYVFTGVLSAEELDDLRSDVEDMWSRLPVSRGAAVDALGRPAL
                     GADLRAPTVQWARPLSDPLGGTDKANGRHPAKMFEPQPPADAPPFVCQLVLGSLQFSD
                     ACLRVYGHPQLLAVAAAINGDDFAPFNEAVWRKEPGLGPSVAWHQDGTTHWDSPDLDE
                     GTHGFNFMAQIYGCTAANAVWVVPGSHARGKVDIAALVARAGSDRLPDAVPLICDPGD
                     VAICNRQAVHGSFANTGGDLRVTVNFGFHRRQSLLGVEGGGIHNRREIYDERRIRERS
                     RVIGYAIDARRQRFPDEAPYVYRPFAGEEDEYRWSDAARARLKDYNLLDLGI"
     gene            complement(4557..6185)
                     /locus_tag="AC16PP_14260"
     CDS             complement(4557..6185)
                     /locus_tag="AC16PP_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007944983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR6482839.1"
                     /translation="MPEGPERFDAVVVGAGFAGLYMLLRLRELGMRVVVFEAGDGVGG
                     TWYWNRYPGARCDAESMGYSYSFSPELEQEWEWTERYATQPEILRYADHVAERFDLKR
                     DIRFETRVVAANYREAGNVWAVATDRGDEVEARFCIMATGCLSVPQRPDIPGLDDFQG
                     PTYVTGEWPHGGVDFTGLRVGLVGTGSSAIQAVPVIAAQAEHLTVFQRTANFSVPAKN
                     RPLDPDWVREFKERYRDYREDSRHGLVSDFGALVLPDAERKRAGGSFRSVPAAERDAR
                     YEKLWEVGGAYFMAAFEDTLLDPEANESAAEFVREKIREIVEDPETAERLCPTSHPIG
                     TKRICVDTGYYETYNRPNVTLVDISGTPIDTVTPKGLRVRNVDYEFDVLVLATGFDAM
                     TGALLGMDIRGVGGERLADHWRAGPRSYLGLCVTGFPNLVTITGPGSPSVLSNMMVSI
                     EQHVDWVADCLGHMADRGLTRLEALQQAEDGWVDHVNEVANATLFPKGGSWYLGANIP
                     GKPRVFMPYAGGVGPYRDICDGVAARGYEGFSIT"
     gene            complement(6178..6630)
                     /locus_tag="AC16PP_14265"
     CDS             complement(6178..6630)
                     /locus_tag="AC16PP_14265"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6482840.1"
                     /translation="MTLDELLEIEAIKQMRYLYSHYYDGNMLDELISLFTEDAVCEYG
                     EDFGGDWVGKAQIRERYHDFLYGSGRAVHSQMHAVTNPWIRLKSDTQALGRWYQLNLN
                     VAEGAENPLTLFGIYDDVYRKVDGEWKIHRTRIDFLWPRRHFYGIRDA"
     gene            complement(6647..7615)
                     /locus_tag="AC16PP_14270"
     CDS             complement(6647..7615)
                     /locus_tag="AC16PP_14270"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017904614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR6482841.1"
                     /translation="MTVKTTTAAVAEAIDQEMARDDRVFVAGEDVRVGGPFGTDAGLH
                     AKYGDRRVVNTAISELALAGLGVGAAAVGLRPIIEIMIMDFMCVAMDQIVNQMAKMKY
                     MLGGKPTLPIVVTTHAGIDSSSAAQHSGSLEAWFCHVPGLKVVMPSNAYDAKGLLISA
                     IRDDSPVIFIHNKRCLQTSADVPDEAYTVPLGVADVKREGSDVTIVAIGRMVDEALEA
                     ADELQGDGVSAEVIDPRTLSPLDMETILDSVRKTTRAVVAHEAVRFCGIGAEIAATIS
                     DEAFDYLDAPVKRVAAPFTPIPFAPVLENLYLPTRESILAAVREIV"
     gene            complement(7633..8595)
                     /locus_tag="AC16PP_14275"
     CDS             complement(7633..8595)
                     /locus_tag="AC16PP_14275"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR6482842.1"
                     /translation="MDLPEEQMIRMLETMVRIREFDERAALLMEQARIHGSVHLYCGE
                     EAVGVGVCEALNPDDYITSTHRGHGHLIAKGGDPRLMYAELFAKSTGYNRGKGGSMHI
                     AALELGMIGANGIVGAGAPIALGVAFASAYQDNGRVTACFFGDGATNEGAFHEALNMA
                     AILKAPVVFVCENNLYGEWTPASQQTSVRDIADRSASYGIPGVIVDGMDIMAVHEAVV
                     EAVARARRGEGPTLLECKTYRFYDHVGRDFGILKRDPDEVAAWRARDPITLWRAELIA
                     QGVLDEAGADAITERAQAEMEDAIAYAEGSPDPEPEDLMRDIYS"
     gene            complement(8677..9564)
                     /locus_tag="AC16PP_14280"
     CDS             complement(8677..9564)
                     /locus_tag="AC16PP_14280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6482843.1"
                     /translation="MRLGFSIPNNQGVERVSDLVRLAVEAEELGYASVWVSEHLWHAG
                     YVARRLGEKPYHEPLTVLTAIAAATRQVRLGTSVLVLPWHHPVRLAKTIATLDDLSDG
                     RVSLGVGVAVTEDEFENLGVDFATRGRRADDALAAIKALWTQEFPRHEGPFYQFSGMR
                     FEPKPRQSPHPPILVGGGAPPALRRAARFADGWHALGQSPESLAESMKMLEESCRAVG
                     RDVADLDISIRCVIDIVGEPWDRPVTQRRTLKGTEREIAETIAAYAEAGVDEIVLDAN
                     LTDLEATRAVMHRGIRFQD"
     gene            9700..>10725
                     /locus_tag="AC16PP_14285"
     CDS             9700..>10725
                     /locus_tag="AC16PP_14285"
                     /inference="COORDINATES: protein motif:HMM:NF047238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR6482844.1"
                     /translation="MQADIDATAGRHLRGRYAVVGVGETGYVRGSGCTTRALGTRAVR
                     AALADAGLEPGDVDGMLSYSYNDSTFSPFIAGDLGIRLNFYMDVYGGGSSIEALIGIA
                     MGVIEAGMCRTVVIFRAMNGYSQVRIGGTGGRGAVAPLGGDALFNRAFGQMSAGQMFC
                     QSFMRHMYDYGTTPEQVAMVKVVHSEHASNNPKAYYRQRVSVDDVVNSRMIVKPLHLL
                     DCCVETDNGTAVVVTGIDRARDCRHTPAVIQSVVGRCSKRRSDMHYQSGPISTVAGHY
                     AKDILWPNAGVGPEDIDVTGSYDAFTFTTMLQLEDYGFCEKGAGGEYVSSGTIELGGA
                     RPNNTSGG"
BASE COUNT         1875 a         3635 c         3482 g         1733 t
ORIGIN      
        1 cgcaacggaa ggcctgggct tcacgggctg caccaccgag gagtggcttg cgcacaaggg
       61 caccgtgggc cgggtgctgc tgggagagct gcacatcctc gacgaggatg gcgacccttc
      121 cccgaaaggc gagccaggcg aggtctggtt caagaccgcg tcggccttcg agtacttcaa
      181 cgatcccgag aagacggcgc aggccacctc ggcagacggc agcatgacca ccgtgggcga
      241 cgtcggctac gtggatgacg acggctacct gtacctcacc gaccgggcga cgttcatgat
      301 catctccggc ggggtgaacg tctatcccca ggaatgcgag aacctgctga tcacccatcc
      361 gaaggttgcc gacgccgcgg tgttcggcgt gcccaacgtg gatctcggcg aagaggtgaa
      421 agccgtggtc caggtcatgc cggacgtgga accgggggaa gcgctggaag cggagctcat
      481 cgccttctgc cacgagcacc tcgcccgcca gaagtgcccc cgctccgtcg acttcgagga
      541 acagctgccc cgcctgccta cgggcaagct ttataagcgt cacctgcgcg accggtactg
      601 gggcgacaaa aaaagccgca tcgtctgagc tgaaacaggc aggcgggcgc ccgcctcaac
      661 gggggagttc ggcaagaatt cctgatggct cggtgagctt cggctccagc aacgccagca
      721 ggctgccgcg atccaggctg ccgcctgcga ccagctctcc cacaacggcg tcgaggctgc
      781 tgcccgcatc ctcgagctcc gcagcgagct catgaaacgt caatgccgcg cgggccgtgt
      841 gcgcacccgt ggatggatcc gccaggcgcc cgccgtcgcg ttgcgcccag gccgccagtg
      901 accccagggc cgacgcgaag cgctcctcgc tgataccccc gctgcgccga agggtctcca
      961 ggctgtagta ctcggcaagc ccttccatga tccagtcgtc gccagccgat ttcggcgccg
     1021 tcgtggcgat gtgcacgagc tcatgaagca gcgtgctggt gccgttctcg ctgatgagcg
     1081 ggcgcccgac gtggaggtac agcgattccg ggccggaaag gccccctcgc cacatgccct
     1141 ccggggcacc tacgacgagc agccggtcgg gcattccggg aaacacggcg acgagatcgg
     1201 gcagcgtcca gcgcaggaag gcgagcacgt ccacccggcg aaagccctgc cccaccggcg
     1261 ccgccactga aaccgtccgg ccggcgatgg tatccctgcg gatgccgagc ctgccggcca
     1321 ccagccagcc ggtgggccgg tcgaaccggc gcccctcgtc caccacggcc acctgctcgt
     1381 ggctcggccc gtagcgggtc tcgaaccgcc agcccgccgg tccggcaaga acgacgctcg
     1441 accgggattg cgcccccgac tcgctgcgcg tccgggcgcg cggaaagaga tcatccagcc
     1501 gcgcgatggc ccaatccggc gtcatccgcg catcgtaggc cccgttgcgg cgatggtcga
     1561 tcaacacgtc gtagctcagg gtgccgccgt cctcaggaac ggtccagacc agacgatctt
     1621 cctcgcgccg gatgctcccg ggacctgaaa aggccgaata ggtcgccgcc ggggccgcca
     1681 ggtccaggcg cttgagctcg cccgcctcct gagtaatggt gatcgaggca tgcgccgttc
     1741 ccgcttcggg aagcaggtgc gccctgaaat cagcgccgta agtcccgtcg gcgtgagcgg
     1801 gttgagcggc gagcagcacc atcgccgcca ccagggtgac cctcagggtc tcaacgttca
     1861 gcatcgtttg acgactccac acggcacctt cgcgataacg gctggatgac acagcgcaga
     1921 taccagagcg ccgtcgggat tggaagcgcg ccgacgtttg aatcgagaaa ttgaatctcc
     1981 catagtgtac gcgcggacgc gactggcgct gagaggagcg aagcgatgat cgacgtgtat
     2041 tactggccta ccccgaacgg ctggaaggtc accatcatgc tggaggagtg cggcctgccc
     2101 taccggatac tgccggtcga catcggcggc ggcgaccagt tcacgccgga ctttctgcgc
     2161 gtgagcccca acaaccgcat gccggccatc gtcgatcacg agcctctcgg cggcggggag
     2221 ccgctgtcga tcttcgaatc cggcgccata ctcgagtatc tcgccgacaa gtcggggaag
     2281 tttctgtccc gggagccggc cggccgatac cgcaccctgc agtgggttca ctggcagatg
     2341 gccaatctgg gcccgatgat gggcaacgcc aatcacttca agaactacgg caagaacatc
     2401 gccgaggacc caaagcagct ggaatacggc acccgtcgtt tcgtgggcga agtggaccgt
     2461 ctgtccggcg tgatggacgc tcagctccgc gtcaaccggt atctcgccgg cagcgagtac
     2521 accatcgcgg acatcatcac ctggccgtgg gccatgctgc tcggccgcct catcgacgag
     2581 gccgtctggg agacctttcc gaacctcaag cgctgggtgg acgaggtggg cgaacgcccc
     2641 gccgtggaaa agggccgcaa agtggaacgc gatctcggcc gccaggagcg gaccgaggaa
     2701 caggagaaag cccggcggga gctcctgttc aaccagacca acgaaaaagt gcgtgtggca
     2761 cgagaggccg cggcgaaagc ggccgccggc tgacgggccg gacctcgcca cgcgctcgca
     2821 atgcgcagcg cgtcgtgatt aagtgtgatt cttcgagacc gcattcaaga ccaagaaggg
     2881 agggaagcaa atgatcagca gtggaccact ggaagaccgc atggcaattc gcgagctagt
     2941 cgactcgtac aacgatgccg tcatgcgttt cgatggagat gcgtgggcgg ccaactggcg
     3001 cgatgacgcc acctggtctc tgcccggggc cggaccgatc aagggaaaag agaacttctt
     3061 tcccatgtgg cagcaggcca tgagcgcgtt ctccttcgtc ggcttcttcg cctcggccgg
     3121 gcctatcgtg gtggatggcg acaccgctca cgccacctgg taccagcagg agtttctcca
     3181 ccagaaggac ggcgtgaaac gcaacgtcac gggacgttac gaggacgact acgtcaaggt
     3241 agatggccgc tggtacttcc agcagcgcat ctacgaggtc atgaacgccg aagagtccta
     3301 ggcgccttaa atccccaggt cgaggaggtt gtagtccttg agccgcgcgc gggccgcgtc
     3361 gctccagcgg tactcatcct cctcgcctgc gaacggtcga tagacgtagg gcgcctcgtc
     3421 gggaaagcgc tgcctgcgcg catcgatggc gtaaccgatc acacgcgacc gctcgcggat
     3481 acgccgttca tcgtatatct cccggcggtt gtggatgccg ccgccctcca cgccaagcag
     3541 cgattggcgt cggtgaaatc cgaagttgac ggtgaccctc agatcgcccc cggtattggc
     3601 gaaggatccg tgtaccgcct gacggttgca gatggccaca tctccgggat cgcagatgag
     3661 gggcaccgcg tcgggaaggc gatccgagcc tgcgcgggcg acgagcgcgg cgatgtcaac
     3721 ttttccgcga gcgtgtgatc ccggcacgac ccagacggcg ttcgccgccg tgcacccgta
     3781 aatctgcgcc atgaaattga atccgtgggt tccctcgtcg agatcgggac tgtcccagtg
     3841 ggtggtaccg tcctggtgcc aggcgaccga cgggcccaga cccggctcct tccgccagac
     3901 ggcttcgttg aacggcgcga aatcgtcccc gttgatggct gccgccaccg cgagcagctg
     3961 aggatggccg taaaccctga ggcacgcatc ggagaactgc agggacccga gcaccagctg
     4021 gcagacgaaa ggcggcgcat cggccggcgg ctgcggctcg aacatcttgg ccgggtgccg
     4081 accgttggct ttgtccgtgc cgcccagggg atcgctgagg ggccgcgccc actgcaccgt
     4141 gggtgcgcga aggtcggcgc ccagcgcggg acgacccagc gcgtccaccg cagcaccccg
     4201 ggagaccggc agtcgcgacc acatgtcctc gacgtccgaa cgaagatcgt ccagctcctc
     4261 ggccgacaac accccggtga acacgtagaa gcccaggcgc gagtaggcgt cgaggatgtc
     4321 cgccgcgagc cgaccatcgg cgccgaagcg gatgggcccc cggttaccca gggcaagggc
     4381 gcgcgcctcg ccttcgcgaa gataggcgcc catggcctcc tcatggggcc cgtagtcagg
     4441 gctggtgtct tggttcgcct cagccatacc tatctcccga tcgatccgca tcgcggtgcg
     4501 cgcccccgaa gtatcgcctc gggcaagcct tgccgtcgac ccgcgctgcg gcgccgctag
     4561 gtgatcgaaa agccctcgta gccgcgcgcg gccacgccat cgcagatatc acgatacggg
     4621 cccacgccgc ccgcataggg catgaacacg cgcggcttgc cgggaatgtt tgcgccgaga
     4681 taccaggacc ctcccttggg gaacagcgtg gcgttggcga cctcgttgac gtgatccacc
     4741 caaccgtcct cagcctgctg cagagcctcc aggcgggtga gcccgcgatc ggccatgtgg
     4801 cccaggcagt cggccaccca gtccacgtgc tgctcgatgg aaaccatcat gttgctcaga
     4861 acagaggggc tgcccgggcc ggtgatggtc accaggttgg gaaacccggt gacgcacagg
     4921 cccaggtagc tccgcgggcc cgcacgccag tgatcggcca gccgctcccc cccgacaccc
     4981 cggatgtcca tgccgagcag cgcgccggtc atggcgtcga agcccgtggc gagcaccaga
     5041 acgtcgaact cgtagtcgac gttgcgcacc cgcaaccctt tcggcgtgac ggtatcgatg
     5101 ggggtaccgg agatgtccac cagcgtcacg ttggggcggt tgtaggtctc gtaatagccg
     5161 gtgtccacgc agatccgttt ggttccgatg ggatgcgacg tggggcacag gcgctctgcg
     5221 gtttccggat cctcgacgat ctcacggatc ttctcacgca cgaactccgc cgcgctctcg
     5281 ttggcctcgg gatccagcag ggtgtcttcg aaggccgcca tgaaatacgc gccgccaacc
     5341 tcccagagct tttcgtagcg ggcatcgcgc tcggccgccg gcacggaacg aaacgatccg
     5401 ccggcccgct tgcgctcggc gtcgggcagc acgagggcgc cgaagtcgga caccagcccg
     5461 tgtcgcgaat cctcacgata gtcccggtag cgctccttga actcgcgcac ccagtcggga
     5521 tccagcggac ggttcttggc gggaacgctg aaattcgccg tgcgctgaaa taccgtcagg
     5581 tgctcggcct gggcggcgat gacgggcacg gcctggatgg ccgatgagcc cgtgcccaca
     5641 aggccgaccc gcaggccggt gaaatccacc cccccgtgag gccattctcc ggtcacgtac
     5701 gtcgggccct gaaagtcatc caggcccggg atgtcgggac gctggggaac ggacaggcac
     5761 ccggtcgcca tgatgcagaa gcgggcttcc acctcgtctc cgcggtccgt ggccacggcc
     5821 cagacgttgc cggcttcccg atagttagcc gccaccaccc gcgtctcgaa gcgaatgtcc
     5881 cgcttgaggt cgaagcgctc ggcgacatgg tcggcgtagc gaagaatctc gggctgggtg
     5941 gcgtagcgct cggtccactc ccactcctgc tcgagctcgg gcgagaacga gtacgagtag
     6001 cccatgctct ccgcgtcgca gcgggcgccc ggataccggt tccagtacca ggtgccgccg
     6061 acgccgtcac ccgcctcgaa aaccaccacc cgcatgccca gctcacggag ccgcagcagc
     6121 atgtaaagcc cggcaaagcc ggcgccgacg acgacggcgt cgaagcgttc cgggccttca
     6181 ggcatcccgg atcccgtaga agtggcgccg gggccagagg aagtcgatgc gggtcctgtg
     6241 gatcttccac tccccgtcca ccttgcgata cacgtcgtcg tagatgccga agagggtcag
     6301 cgggttctcc gcgccttcgg cgacgttcag gttcagctgg taccagcggc cgagcgcttg
     6361 ggtatcgctc ttcaaacgga tccacggatt ggtcaccgcg tgcatctggg aatgcaccgc
     6421 gcgtccgctg ccatagagga agtcgtggta acgctcgcga atctgcgcct tgcccaccca
     6481 gtcgccgccg aaatcctcgc cgtactcgca tacggcgtcc tcggtgaaga ggctgatcag
     6541 ctcatcgagc atgttgccgt cgtagtagtg cgagtacagg taacgcatct gcttgatggc
     6601 ttcgatctcg agcaactcgt caagggtcac ggccggctcc tcctgttcag actatttccc
     6661 gaacggccgc caggatggat tcccgggtcg gcagatagag gttttccagg accggcgcga
     6721 agggaatcgg cgtgaacggc gcggccaccc gcttaaccgg cgcgtcgagg tagtcgaacg
     6781 cctcgtcgga gatggtcgct gcgatctcgg caccgatgcc gcagaacctg accgcctcgt
     6841 gggccaccac cgcccgcgtc gtcttgcgaa cggagtcgag aatcgtctcc atgtccagcg
     6901 gcgacagggt ccgcgggtcg atcacctcgg cgctgacacc atcgccctgc agctcgtcgg
     6961 ccgcttccag agcctcgtcg accatgcggc cgatggccac gatggtgacg tcgctgccct
     7021 cccgcttgac gtcggccacg ccgaggggaa ccgtgtaggc ctcgtcgggc acgtcggccg
     7081 aggtctgcag gcagcgcttg ttgtgaatga agatcaccgg gctgtcgtcc cggatggcgg
     7141 agatcagcag gcccttggcg tcataggcgt tggaaggcat gaccaccttc aggccgggca
     7201 cgtggcagaa ccacgcctcg aggctgcccg agtgctgggc cgcgctgctg ctgtcgatac
     7261 ccgcgtgggt cgtcaccacg atgggcagcg tgggctttcc gccgagcatg tacttcatct
     7321 tggccatctg gttcacgatc tggtccatgg ccacgcacat gaagtccatg atcatgatct
     7381 cgatgatggg tcgcagaccc accgccgcgg ccccgacccc gaggcccgcg agggccagct
     7441 ccgatatcgc cgtgttcacc acgcgccgat cgccgtactt ggcgtgaaga ccagcgtccg
     7501 tgccgaacgg cccgcccacg cggacgtcct ctccggcgac gaacaccctg tcgtcccgcg
     7561 ccatttcctg gtcgatggcc tctgccaccg cggccgtcgt cgtctttact gtcaccctgc
     7621 ccccttcgat attcagctgt agatgtcgcg catcaggtcc tcgggctcgg gatccgggct
     7681 gccctccgca tacgcgatgg cgtcctccat ctccgcctgg gcgcgctccg tgatggcgtc
     7741 ggcgccggct tcgtcgagca ccccctgagc gatcagctcg gcccgccaga gcgtgatggg
     7801 gtcccgggcg cgccatgcgg ccacctcgtc gggatcccgc ttgagaatgc cgaagtcgcg
     7861 cccgacgtgg tcgtagaagc ggtacgtctt gcattccagg agcgttggcc cctcgcccct
     7921 gcgggctctt gccacggctt ccaccaccgc ctcgtgaacg gccatgatgt ccatgccgtc
     7981 gacgatgacc cccggaatgc cgtatgaggc cgaacgatcg gcgatgtccc gcaccgacgt
     8041 ctgctgggaa gccggcgtcc actcgccgta gaggttgttc tcgcaaacga agaccaccgg
     8101 cgccttgaga atggcagcca tgttgagcgc ttcgtgaaaa gcgccctcgt tggtcgcgcc
     8161 gtcgccgaag aaacaggccg tgacccggcc gttgtcctga taagcgctgg cgaaggcgac
     8221 gcctagcgcg atgggcgccc ccgcgccgac gatgccgttg gcaccgatca tgcccagttc
     8281 cagcgcggct atgtgcatgg agccgccctt accccggttg tagccggtgg acttggcgaa
     8341 caactctgca tacatcagcc ggggatcgcc gcctttggcg atgaggtgcc cgtggccccg
     8401 gtgtgtcgag gtgatgtagt cgtccgggtt cagcgcttcg cagacgccga cgccaacggc
     8461 ctcctccccg caatacagat gcaccgagcc gtggatgcgg gcctgctcca tgagcagcgc
     8521 agcccgctcg tcgaactcgc ggatccggac catggtctcg agcatgcgaa tcatctgctc
     8581 ttcgggaaga tccatggcag cgctcctgag aataagacat cgaaggctaa cgacgcccgc
     8641 caaccaggtc aaacagcgcc gcaaggagcg gccggctcag tcctggaacc ggattccccg
     8701 gtgcatgacg gcgcgggtag cctccaggtc ggtcaggttc gcatcgagaa cgatctcgtc
     8761 cacgcccgcc tcggcgtagg cggcgatggt ctccgcgatc tctctctccg tgcctttcag
     8821 cgtccggcgc tgagtcaccg gtcgatccca gggttcccct acgatgtcga tgacgcagcg
     8881 aatgctgatg tcgagatccg ccacgtcccg acccaccgcg cggcacgatt cctccagcat
     8941 tttcatcgac tccgccaggg actccgggct ctgcccgagc gcgtgccagc catctgcgaa
     9001 ccgcgcggcg cgccggagtg cgggcggcgc gccgccgccc accaggatgg gcgggtgcgg
     9061 cgattgccgt ggtttcggct cgaaacgcat cccggagaac tgatagaagg ggccttcgtg
     9121 gcggggaaac tcctgggtcc agagcgcctt gatggcggcc agcgcgtcgt ccgcgcgccg
     9181 gccccgggta gcgaagtcga cgccgaggtt ctcgaactcg tcctcggtga ctgccacccc
     9241 gacccccagg ctgaccctgc cgtcggagag gtcgtccagc gtggcgatgg tcttcgccag
     9301 ccgaaccgga tgatgccagg gcagcacgag cacggacgtg cccaggcgga cctgccgggt
     9361 ggcggcggca atggcggtga gcaccgtcag gggttcatga tagggcttct cgcccaggcg
     9421 cctggccacg tagcctgcgt gccagagatg ctcgctcacc cacaccgacg cgtaaccgag
     9481 ctcctccgcc tcaaccgcca gccgcaccag gtcggagacg cgctccacgc cctggttgtt
     9541 cggaatgctg aatccaagcc gcatagctcg aaacgggccc ccagccctgc ctccggtcct
     9601 gcccggattg gttcggcgag tatttcacga tcgccggtac tcccgcacct cgattagtgc
     9661 taaaagaaac ggcaggcagc tttgggagag gagcagccca tgcaggctga catcgacgca
     9721 accgccggac ggcacctgcg cggcaggtac gccgtggtgg gcgtgggcga gaccggttac
     9781 gttcgcgggt ccggctgcac gacccgcgcc ctgggcaccc gcgccgtccg ggccgccctc
     9841 gccgatgcag ggcttgagcc aggcgacgtc gacggcatgc tgtcctacag ctacaacgac
     9901 tcgaccttct ctcccttcat tgccggcgac ctggggatcc gtctgaactt ttacatggac
     9961 gtctacggcg gcggctcgag catcgaggcg ctgatcggga tcgccatggg cgtcatcgag
    10021 gcgggcatgt gtcgcacggt ggtcatcttc cgggccatga acgggtacag ccaggtacgt
    10081 atcggtggca ccggcggccg cggcgcggtg gcgccactcg gcggtgacgc gctgttcaac
    10141 cgggcgttcg gccagatgag cgcggggcag atgttctgcc agtcgttcat gcgccacatg
    10201 tacgactacg gcaccacgcc ggagcaggtc gccatggtca aggtcgttca ctcggagcat
    10261 gcctccaaca accccaaggc ctactacagg cagcgggtga gcgtggacga cgtcgtcaac
    10321 agccgcatga tcgtcaagcc cctgcacctg ctggactgct gcgtggagac ggacaacggc
    10381 acggccgtcg tggtcaccgg catcgaccgc gccagggact gccggcacac gccggcggtg
    10441 atccagtcgg tggtggggcg ctgcagcaag aggcggtccg acatgcacta ccagtcgggg
    10501 ccgatttcca ccgtggccgg ccactacgcg aaggacatcc tctggcccaa cgcgggcgtg
    10561 ggcccagagg acatcgacgt caccggttcc tacgatgctt tcaccttcac caccatgctg
    10621 cagctcgagg actacggctt ctgcgagaag ggcgcgggcg gcgagtacgt gtcgagcggc
    10681 accatcgagc tcggcggcgc gcggccgaac aacacctccg gcggg
//
LOCUS       DBNHKV010000436         5203 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_771314, whole genome shotgun sequence.
ACCESSION   DBNHKV010000436 DBNHKV010000000
VERSION     DBNHKV010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_771314"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="AC16PP_14290"
     CDS             <1..646
                     /locus_tag="AC16PP_14290"
                     /inference="COORDINATES: protein motif:HMM:NF012379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR6482845.1"
                     /translation="PEIAFADLNANAELAEDFLKAVCRRVLDACPDDMSFFADRVDEQ
                     AISRLEHVIATPFERIDYTDAVSILEKAGQNFEYPVQWGLDLQSEHERYLTEVHVGGP
                     VVVVNYPKTIKAFYMRLNDDDATVAAMDVLVPGVGEIIGGSQREERLDVLDARMAEQD
                     IGEDLWWYRDLRRYGTAPHAGFGLGLERLVLYMTGMDNIRDAIPFPRVPHNASF"
     gene            671..2431
                     /locus_tag="AC16PP_14295"
     CDS             671..2431
                     /locus_tag="AC16PP_14295"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6482846.1"
                     /translation="MIRSLIRLKSFLAPYRLRLGAGVGAFGIARFFEAMVPIFVAIGI
                     DRMSAGNADMTTPLLGIAGAVIGRYVVVTYARYSVRCVGQHVAFDLRQALYESLQQQG
                     VRFFTRYTIGDMMTRAVADIGLIQRLIAMGTILMVILVYATLVGFGFMLYLSPSLTLL
                     LLPPLPLVFFYASWSARQMGVASRDVQNRLSDLGAHAQENLSGIRTIQAMVQEENEIR
                     RFGRTNQQYANAFYRQSQINSLMAAWMPSLAAICSVTILGYGGYLVLDGAMTVGELVA
                     FFMYVNMVVQPFRVAGFIVNLFQRAAVASDRLFEVITLSPEIADDPSGTTPATIRGDV
                     EIRHLTFAYDEGIRPALDDVSLTVARGESVTIMGRVGSGKTTLLKQLVRLLDTPPGAV
                     LIDGHDVRDYPLAQLRSQVAMVPQDAFLFGEPLGDNLTYDDPGRAVEKIWEAATSADL
                     KETIEEFPEQLDTIVGERGVTLSGGQKQRATLARGLIRGAPVLILDDCFSSVDTETEE
                     HILRELRRLRRRQTTILVSHRVSTARHSDRIVVMDAGRIVESGTHEELLARAGQYAEL
                     ERIQREGAVDTDYDLIGATT"
     gene            2428..4305
                     /locus_tag="AC16PP_14300"
     CDS             2428..4305
                     /locus_tag="AC16PP_14300"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6482847.1"
                     /translation="MTAASQPGAPERRNYSMFDAEISGAVLNLALLRRLLKWLRPYRV
                     TFGISAVLVLLASTLQVLMPIIISLVVIDHLIRGETDSLAPDLGMVELTEWLADILAL
                     HPLMAACLLYAGLQIAWAAAGHAHRMTLISSVINGLRDLRLDLFRHLETRPSSFYDRV
                     AVGRVMTRVTNDIEALYELLRGLGSLIGEFMPFFVALAIMLAIDVELTLLLLLALPVM
                     GSVTFYFRRATRRLFRMVRMSLSALNQNMQENLAGLQVVQLSERQQYNLDRYTKINEE
                     NRGHELRTTRFETLYGAFNDSMAHLALGVIIWYAGGAAVQEEMTLGAVILFTRFIDML
                     FHPIVVLGEQTNILFRAMASGERIFQALDWDEKIHEPADPVTLPPRLQGEVTFRHLNF
                     AYDPDVQILHDVSFEIGAGEKLAIVGPTGSGKSTLIRLLGRFYEFDDGQIFLDGIDLN
                     RIHTRDLRQRIGVVLQDFHIFSGTVFDNIALGDPRITRERAEEAARRVNAHGFISDLP
                     RGYDTELSERGQNLSQGQRQLLAFARVLAADPEILVLDEATASIDTETELLIQDALRK
                     LTAGRTSIIIAHRLQTIQEADRVLVLQGGRVVELGTHEELIASEGLYHTLHSLQFQDE
                     R"
     gene            complement(4307..5029)
                     /locus_tag="AC16PP_14305"
     CDS             complement(4307..5029)
                     /locus_tag="AC16PP_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019689442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6482848.1"
                     /translation="MNDRPVIVTGGAGGIGSAIVARLVADGHPVAVLDLHPASATPSG
                     SLFSYPCDIADLDAVERAVAEFEAAVGAPFGLVNNAGWDQALPFLETGPDLWEKVIDV
                     NLRGPVNVTYAVLRRLAGAGRGRVVSIASDAGRVGSSGEAVYSACKGGIIAFMKTLAR
                     EHARQGITFNSVCPGPTDTPLLDSFDESGRLAQALERAIPMRRLGRPEDFPGIVAFLL
                     SDEASFITGQTVSVSGGLSMQG"
BASE COUNT          914 a         1783 c         1616 g          890 t
ORIGIN      
        1 accggaaatc gccttcgccg acctgaacgc caacgcggag cttgccgagg acttcctgaa
       61 agccgtctgc cgcagggtgc tggacgcctg tcccgacgac atgagtttct tcgccgaccg
      121 ggtcgacgag caggccatca gccgcctgga acacgtcatc gccacaccgt tcgaacggat
      181 cgactacacg gacgccgtca gcatcctcga gaaggcgggt cagaacttcg agtacccggt
      241 gcagtgggga ctcgacctgc aatccgagca cgagcgctat ctcaccgagg tgcacgtggg
      301 cgggccggtg gtggtggtca actaccccaa gaccatcaaa gcgttctaca tgcgcctgaa
      361 cgacgacgac gcgacggtgg ccgccatgga cgtgctcgtc cccggcgtcg gcgagatcat
      421 cggcggcagc cagcgggaag agcgcctgga cgtgctcgac gcccgcatgg cggaacagga
      481 catcggcgag gatctctggt ggtaccggga ccttcgccgg tacggcaccg caccccacgc
      541 ggggttcggg ctcggtctgg aacgcctggt gctttacatg acggggatgg acaacatccg
      601 tgacgccatc ccgttcccca gagtgccgca caacgccagt ttctgacgcg gcagcggctg
      661 cgccccaccg atgattcgct ctctgatccg tctgaaatcg ttcctcgccc cgtatcggct
      721 gcgactgggg gccggcgtcg gcgcgttcgg aatcgcaaga ttcttcgaag ccatggtgcc
      781 tatcttcgtg gccatcggca tcgatcggat gtccgccggc aacgcggaca tgacgacccc
      841 actgctcgga attgccggag cggtgatcgg gcgctacgtc gtggtcacct acgcccgcta
      901 ctccgtgcgc tgcgtcggcc agcacgtggc gttcgatctg cggcaggctc tgtacgaatc
      961 cctgcagcaa cagggcgttc gtttcttcac gcgctacacc atcggcgaca tgatgacccg
     1021 ggcggtagcc gacatcggcc tgatccagag actcatcgcc atgggcacga tcctgatggt
     1081 catcctcgtc tacgcgacgc tggtgggctt cggcttcatg ctctatctgt ccccgtcact
     1141 cacgctgctg ctgctgccac ccctgcccct ggtgttcttc tacgcaagct ggtccgcgcg
     1201 ccagatgggc gtggcctcga gggacgttca gaaccgattg tccgacctgg gcgcccacgc
     1261 ccaggaaaac ctctccggga ttcgcaccat ccaggccatg gtccaggaag agaatgagat
     1321 ccgccggttc ggcaggacca accagcagta cgccaacgcg ttctaccgcc agtctcagat
     1381 caactcgctc atggcggcct ggatgccttc gctggccgcc atctgctccg tcaccatcct
     1441 ggggtatggc ggctacctgg tcctggacgg cgccatgacg gtgggcgagc tcgttgcctt
     1501 cttcatgtac gtgaacatgg tcgtccagcc gtttcgggtg gccggcttca tcgtcaacct
     1561 cttccagcgg gcggccgtgg ccagcgaccg gctgttcgag gtcatcaccc tgtccccaga
     1621 gatcgccgac gatccaagcg ggacgacgcc ggcgacgatc cggggcgacg tcgagatacg
     1681 ccacctgacc ttcgcttacg acgagggcat tcgtccggct ctcgatgacg tctcgctgac
     1741 cgtcgcccgg ggcgagtccg tgaccatcat gggcagggtc ggatcgggca agacgacgct
     1801 gctcaagcag ctcgtgcggc tgctggacac gccgccaggc gcggttctca tcgacggcca
     1861 cgacgtccgc gactacccac tcgcccagct gaggtcccag gttgccatgg tgccccagga
     1921 tgcttttctg ttcggcgagc ccctgggcga caacctgacc tacgacgacc cgggccgggc
     1981 ggtggagaag atctgggagg cggcaacgtc cgcagatctc aaggaaacca tcgaggagtt
     2041 cccggagcag ctcgacacca tcgtcggcga gcgcggcgtg acgctctccg gaggccagaa
     2101 gcagcgagcc accctggccc gcggcctcat tcgcggggcg cccgtgctga tcctcgacga
     2161 ctgcttttcc agcgtcgaca ccgagacgga agagcacatt ctccgggagc tgcgccggct
     2221 gcggcgccgc cagaccacca ttttggtgtc ccaccgcgtt tcgaccgccc gccactccga
     2281 ccgcatcgtg gtcatggacg ccgggcgcat cgtcgagtcc ggtacccacg aagagcttct
     2341 ggccagggca ggacagtacg ccgagctcga acggattcag cgcgagggcg ctgttgatac
     2401 cgactacgac ctgatcgggg cgacgacgtg acggcggcca gccagcctgg cgctcccgag
     2461 cggcgcaact actccatgtt cgacgccgag atctccggcg cggttctgaa ccttgcgctc
     2521 ctgcgacgcc tcctgaaatg gttgcggccc taccgggtga ccttcggaat cagcgccgtg
     2581 ctggtgctgc tggcgtccac gctgcaggtt ctcatgccca tcatcatctc gctggtggtg
     2641 atcgaccacc tgatccgggg cgagacggat tccctggcac cggacctcgg catggtggag
     2701 ctgaccgagt ggctggcgga catcctggca cttcaccctc tcatggcggc ctgtctcctg
     2761 tatgcggggc tgcagattgc ctgggcggct gccggtcacg ctcaccgcat gaccctgatc
     2821 tcgtcggtca tcaacggtct gagggacctg cggctggatc tgtttcgaca cctcgagacc
     2881 agaccgtcct cgttctacga ccgagtcgcc gtaggacggg tcatgacgcg ggtgaccaac
     2941 gacatcgaag cgctctacga gctgcttcgc ggtctcggct cgctcatcgg cgagttcatg
     3001 cccttcttcg tggcgctcgc catcatgctg gccatcgacg tcgagctaac cctgcttctg
     3061 cttctcgcgt tgcccgtcat gggcagcgtg accttctatt tccgccgggc gacgcgccgc
     3121 ctgttccgga tggtgcgcat gtcgctgtcg gctctgaatc agaacatgca ggaaaacctg
     3181 gcgggccttc aggtggtgca gctctccgag cgacagcaat acaacctgga ccgttacacg
     3241 aagattaacg aagaaaaccg cggccacgag ctcagaacga cgcgattcga gaccctctac
     3301 ggcgccttca acgacagcat ggcgcacctg gccctcgggg tgatcatctg gtacgccggc
     3361 ggcgctgcag tgcaggaaga aatgaccctg ggcgccgtga ttctgttcac ccgcttcatc
     3421 gacatgctct tccatcccat cgtggttctc ggcgagcaga cgaacattct cttccgggcc
     3481 atggcgagcg gcgagcggat ctttcaggcg ctggactggg acgagaagat tcacgagccg
     3541 gccgatcccg tcacgctgcc gccccggctg cagggcgagg tcaccttcag gcacctgaac
     3601 ttcgcctacg acccggacgt tcagattctc cacgacgtct ccttcgagat cggggccggg
     3661 gagaagctcg ccatcgtggg cccgacgggg tccggcaaga gcacgctgat ccgcctgctc
     3721 ggaaggttct acgaattcga cgacggccag attttcctcg acggcatcga tctcaaccgc
     3781 atccatacgc gcgacctgcg ccagcgcatc ggcgtggtgc tccaggactt ccacatcttc
     3841 tcagggaccg tcttcgacaa catcgccctc ggcgatcccc ggatcacccg ggagcgcgcc
     3901 gaggaggccg cgcgccgcgt caacgcccac ggcttcatca gcgacctgcc ccgcggatac
     3961 gacaccgagc tctccgagcg cggtcagaac ctgtcccagg ggcagcggca gctgctggcc
     4021 ttcgcccggg tgctggccgc ggatcccgag atcctcgtgc tcgacgaggc aaccgcgagc
     4081 atcgacaccg aaaccgagct cctcatccag gacgcgctgc gaaagctcac cgccgggcgg
     4141 acttcgatca tcatcgccca tcgcctgcag accattcagg aagccgaccg ggtgctggtt
     4201 cttcagggag gaagggtggt cgagctcggc acccatgagg agctgatcgc cagcgagggg
     4261 ctctatcaca ccctgcactc gctgcagttt caggacgaga ggtaggtcac ccctgcatcg
     4321 acaacccgcc agagacgctc acggtctggc cggtgatgaa cgacgcctcg tcactcaaca
     4381 ggaaggcaac gatccccgga aagtcctccg gtcttcccag ccgccgcatg ggtatggccc
     4441 gctcgagcgc ctgggccagc cgacccgact cgtcgaagga atcgagcaga ggcgtgtccg
     4501 tggggccagg gcagacggag ttgaacgtga tgccctgacg cgcgtgctca cgagccagcg
     4561 tcttcatgaa ggcgatgatg ccgcctttgc acgctgagta gacggcctct cccgacgagc
     4621 ccacacggcc ggcatccgag gcgatgctga ccacccggcc gcggcccgcg cccgccagcc
     4681 tgcgcagcac cgcgtaggtg acgttcacgg ggccgcgcag gttcacgtcg atcaccttct
     4741 cccacagatc cggccccgtc tccagaaacg gcagggcctg gtcccagccg gcattgttga
     4801 cgagcccgaa cggcgcgccc accgccgcct cgaactcggc gaccgcccgc tccaccgcgt
     4861 cgagatcggc gatgtcgcac gggtaggaaa acagggagcc cgaaggggta gcagacgcgg
     4921 ggtgcagatc gagcacggcc accggatggc cgtcggcaac aagacgggca acgatggcgc
     4981 tgccgatgcc gccggcgccc ccggtgacga tcacgggtcg gtcgttcatc gcgattcctc
     5041 catgcttccc gacggggccg tggcgcccgc gcgcagcgcg cccacccgct gatcgtgtcc
     5101 ctgccgcgag atcctataga acccgatgga aaccaacgcc agcacgtaga aaatcatgag
     5161 cgcggggccg taaatgaaac caagatccca tatgacggcg tcc
//
LOCUS       DBNHKV010000437         2848 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_60504, whole genome shotgun sequence.
ACCESSION   DBNHKV010000437 DBNHKV010000000
VERSION     DBNHKV010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_60504"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /locus_tag="AC16PP_14310"
     CDS             complement(<1..1354)
                     /locus_tag="AC16PP_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482849.1"
                     /translation="MLEAVNVSAEVVAAILGISITVVAIIVQLAATRYNHHITEMFVR
                     EPINVVVQAFFVLTTVLCIWVAALGEAELNATWNTVTLVTVTVALAALLPYFGYVFAF
                     ISPVGIIRRIRRKAEHAVAAGHRAETLRTIDQLQDVARSAIDHGDRAIAMAAVEALSD
                     LFRHYRSVRNRLPEGWSRVDGIVREDPDFVALEPSALDDLDTSGLWVEVKIMHQYVSI
                     IRLSVMKMHDVAGRVGIALRQTAIEQIDDPLVLEASFRGMNSLLRASLNARDIRTTFH
                     LLSQYKAVAEDLVSMGHDEDALAAAEHIQSYGQGAFTMNQPFLLEVAAFDLGELIRLA
                     HKNQSAALDRMLDGFLELDHEIKYESQEESLLGVRRAQMQLGAYLISQGDLARAQRVA
                     DDLSSEGASRLDRLQAGLLSDERALFWEFNPRGVNFSYLEPELRPHLNTLMDMVRNEP
                     R"
     gene            complement(1509..1853)
                     /locus_tag="AC16PP_14315"
     CDS             complement(1509..1853)
                     /locus_tag="AC16PP_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HIR6482850.1"
                     /translation="MRRAIGFLILTTVTTVPAWAGDKLEAAVGGGAGGAIGAVVGDEL
                     GDRKGAIIGSAVGAAIGTAIATDGNDGGPDHHHVEAHTAPASTVHVEVGSAGGHPAGR
                     HCPPGQAKKGRC"
     gene            2024..2665
                     /locus_tag="AC16PP_14320"
     CDS             2024..2665
                     /locus_tag="AC16PP_14320"
                     /inference="COORDINATES: protein motif:HMM:NF018712.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HIR6482851.1"
                     /translation="MTDHWHFHSDTFGNCNCAQNCGCQFNLPSTHGFCQFVEGGHIRD
                     GSFNGMNISGFNWAFMMIWPGEIAEGNGRRQIVIDERANDQQRAAIEKIVSGEVGIPG
                     GNHFSVFGSTCSESLETLYLPIEFEMDIAARRARLAIPGLIEARGEPMMDGFSGSEFH
                     IALARPKGSFEFTYAEIGQGFAEVHGELPMSLQGSYGQFCEHNYDHDGLVIPA"
     gene            2662..>2848
                     /locus_tag="AC16PP_14325"
     CDS             2662..>2848
                     /locus_tag="AC16PP_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482852.1"
                     /translation="MSRDPASEGRVSIRTLDQLALWSSLSIVVALSWWYLFLLAQDMA
                     DMAGMPMGDMAGMHPWTA"
BASE COUNT          492 a          904 c          952 g          500 t
ORIGIN      
        1 ttcgcggctc gttgcggacc atgtccatga gcgtgttcag gtggggacgc agctcgggct
       61 ccaggtagct gaaatttacg ccccgagggt tgaactccca gaacagggcc cgttcgtcgc
      121 tcagcagtcc ggcctgcagc cgatccagcc gggaggcgcc ttccgagctc aggtcgtcgg
      181 ccacccgttg ggcccgggcc aggtcgccct gactgatcag gtaggcgccc agctgcatct
      241 gtgcccggcg cacgccgagc aggctctcct cctgggattc gtacttgatc tcgtgatcca
      301 gctccagaaa gccgtctagc atgcggtcca gggcggcgga ctggttcttg tgagccaggc
      361 ggatcagctc cccgaggtcg aaagcggcaa cctccagaag gaacggctga ttcatggtga
      421 aagcgccctg cccgtagctc tgaatgtgct cggcggcggc gagggcgtcc tcgtcgtggc
      481 ccatggaaac cagatcctcc gccaccgcct tgtactggct cagcaggtgg aaggttgtcc
      541 ggatgtcgcg cgcgttcagg ctggcgcgca gcaggctgtt catgccgcga aagctcgcct
      601 ccagcaccag cgggtcgtcg atctgctcaa tggccgtctg ccgcagggcg atgccgaccc
      661 gaccggccac gtcatgcatc ttcatcacgg acagccggat gatgctgacg tactgatgca
      721 tgatcttgac ctcgacccaa agccccgagg tgtccaggtc gtccagggcg ctgggctcca
      781 gggcgacgaa gtcagggtcc tcccgcacga tcccgtcgac ccgggaccac ccctcgggca
      841 gccggttgcg cacggagcgg taatgccgga acaggtcgga cagcgcctcc accgcggcca
      901 tggcgatggc acggtcgccg tgatcgatgg cgctgcgggc gacgtcctga agctggtcta
      961 tggttcgaag ggtctccgcg cggtggccgg cggcgacggc atgctccgcc ttgcggcgga
     1021 tgcgtcggat gatgccgacc ggcgagatga aggcgaagac gtagccgaag tagggcagca
     1081 gcgcggccag ggcaacggta acggtaacca gggtgacggt gttccaggtg gcgttcagct
     1141 ccgcctcgcc cagggcggcg acccagatgc acaggacggt ggtgagcacg aagaaggcct
     1201 gaaccaccac gttgatgggc tcgcggacga acatctcggt gatgtggtgg ttgtagcggg
     1261 tcgcggccag ctgcacgatg atggcgacca ccgtgatgga gatgccgaga atcgcggcga
     1321 cgacctcggc agaaacgttt acggcttcca gcacggatcg ggtccggtgc ttgcgatgac
     1381 gcaagcctac cgcaagtcgt cagaaggcgc gatgcgtgcg gttacgcctg atgtcggttc
     1441 tccggaaccg gatcctgccg gaggcagggg acggctggaa aatgccgtcc ccgatcccta
     1501 tctcgatatc agcagcgacc cttcttggcc tggccgggcg ggcagtggcg tcctgcggga
     1561 tgtcccccgg cagagcccac ttccacatgc acggtgctgg caggtgccgt gtgcgcctcc
     1621 acgtggtgat ggtcgggccc gccatcgttg ccatcggtgg cgatggccgt gccgatggct
     1681 gcgcccaccg cgctgccgat gatcgctccc ttacgatcgc ccagctcgtc gccgaccacg
     1741 gcgccgatgg cgcctccggc accgccgcct acggcggcct cgagcttgtc gccggcccat
     1801 gccggcacgg tcgtaacggt ggtgagaatc agaaatccaa tggctcggcg catgacaggc
     1861 ccctgctttt tgaaacaacc ccgggagtgt aggggggcgt cgacagcaga atcgtgacct
     1921 gggtcatgga ttctcccttg cgcgcgcgcc gtgagcctcg cggcgtgcta gtgatcccag
     1981 ggtgtctgat gtatggtcga gaagttggca aggggacatc gtcatgaccg atcactggca
     2041 cttccacagc gacaccttcg gcaactgcaa ctgcgcccag aactgcggat gccagttcaa
     2101 cctccccagc acccatggat tctgtcagtt cgtcgagggt ggtcacatcc gcgacggatc
     2161 cttcaacggg atgaacatct cagggttcaa ctgggcgttc atgatgatct ggccaggtga
     2221 gatcgccgag ggcaacggcc gccgccagat cgtcattgac gagcgcgcaa acgaccagca
     2281 gcgagcggcc atcgagaaga tcgtctccgg cgaggtgggc atccccggcg gcaaccattt
     2341 ctcagtgttc ggcagcacct gctccgaatc gctggaaacc ctctacctgc ccatcgagtt
     2401 cgagatggac attgccgctc gccgcgcgcg gctggcgatc cccggcctca tcgaggccag
     2461 aggcgaaccc atgatggacg ggttcagcgg cagcgagttt cacatcgcgc tggcgaggcc
     2521 caagggcagc ttcgagttca cctatgcgga gatcggccag ggattcgcgg aggtgcatgg
     2581 cgagctgccc atgtcgctgc agggcagcta cggccagttc tgtgagcaca actacgatca
     2641 cgacgggctc gtcattcccg cgtgagccga gatccggcgt cggaagggcg cgtctcgatc
     2701 cggaccctcg atcagcttgc cctctggtcg tcgctgagca tcgtggtagc gctgtcctgg
     2761 tggtacctgt tcctgctggc ccaagacatg gccgacatgg cgggaatgcc catgggcgac
     2821 atggcgggca tgcatccttg gaccgcgc
//
LOCUS       DBNHKV010000438         4557 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_275045, whole genome shotgun sequence.
ACCESSION   DBNHKV010000438 DBNHKV010000000
VERSION     DBNHKV010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_275045"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="AC16PP_14330"
     CDS             <1..1089
                     /locus_tag="AC16PP_14330"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HIR6482853.1"
                     /translation="HRAALVPQSVKKLVGAGFEVVIEAGTGVPAGYPDAAYVDAGATI
                     GGDRAGLLASADVVLRVRKPEADEIAGLKKGSVHVSFLDPFNERGLVDAMAAQGVTAI
                     SMEMIPRSTRAQKMDALSSQANLAGYVTVVLAANHCPKIFPMMMTPAGTIAPSRVFVI
                     GAGVAGLQAIATAKRLGARVEAFDTRPVVAEQVRSLGGKFVEIDLGEVGQTEQGYAKE
                     LTPEQVELQKEGQKKVIGQSDVVITTAQVFGRPAPRIVSKDMVEAMQPGSVVVDMAVE
                     TGGNVEGSVLNEVVDVGGVKIIGLGNLPSQVARHASDMYSSNLFNLLDEFWSQEESKL
                     DLDPEDEIVRACVITRDGSIVNETIKNL"
     gene            1102..1407
                     /locus_tag="AC16PP_14335"
     CDS             1102..1407
                     /locus_tag="AC16PP_14335"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha part 2"
                     /protein_id="HIR6482854.1"
                     /translation="MEAVFLAFVLMLSIFLGFELISKVPATLHTPLMSGANAISGITL
                     VGAITSAGAGADQIATLLGAVAVGFATINVVGGYLVTNRMLSMFKRKDTGGQSGGRS"
     gene            1404..2816
                     /locus_tag="AC16PP_14340"
     CDS             1404..2816
                     /locus_tag="AC16PP_14340"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIR6482855.1"
                     /translation="MSLELINLWYIVAAALFIFGLKQLSSPDTAVRGNLLSSVGMLIA
                     VVVTLFSRDILEFQWIAAAAAVGAIIGAVAAQRVAMTSMPEMVALFNGSGGIASLLVG
                     WAALYLGDGAAAEAAVADTAVVTVGLSTFTIITILLSVLIGGLTFSGSLIAWGKLSEV
                     MTSRAIVFGAQRLVNAALLIGLVACGVMFAMEPSLTSPYLYAVIALSLIFGVMAVIPI
                     GGADMPVVISLLNSYSGLAACAAGFAINNNILIVAGSLVGAAGIILTNIMCKAMNRSL
                     ANVLFSGFGAVKTATKVEGEVKPITPDDAFLILEAASSVTFVPGYGMAVAQAQHVVRE
                     LGELLEENGAEVTYAIHPVAGRMPGHMNVLLAEANVPYDQLVEMDDINPRIDNIDVAV
                     VIGANDVVNPAAREDENSPIYGMPIINVDQARTVFVLKRSMASGFSGVDNPLFFGENT
                     RMLFGDAKASISSVIAEFKG"
     gene            complement(2940..>4557)
                     /locus_tag="AC16PP_14345"
     CDS             complement(2940..>4557)
                     /locus_tag="AC16PP_14345"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR6482856.1"
                     /translation="DCIFGNPGTTENSLLDRLIDYPGLRYYVALHEGVAVGAASFYAQ
                     ASGKTGIVNLHVAPGLGNGIGMIYGALKANSPLIITAGQQDTRMRMREPLLAHDLVAM
                     AEPVCKWSAEPRSADEIGPMLRRAFKIANEPPRGPVFMSLPVDVMEQETSVGATTAGD
                     LRYGTLADAEGIRRLARWLAGSANPAIVVGDDIAAEGAHRALITLAVQTGAAVWQQGL
                     RAQISFPNVHPNSKGRLPFEAGAIRRALAGHDLILLMGGPFFEEIWFDAENALPEEAR
                     VVHVESSHARLALNLAPDLGLVGNLRTTIEHLVEEIAEGAPDDFGAAAKARNDALAES
                     HRAAGDAAADRIERQGDTSPMTPARALLEISRALPKGAIVVDESITASLEVASRFDYA
                     APGDFFAGSGGGIGQGLAGALGVQVAQPNRRVLAISGDGSAMYSIQALWSAVHHGLPI
                     VFVILSNREYRILKHNLDIYRQRFDAGSNKPYPHMDLTRPALGFPAMAEGMGMEGELV
                     TRPEKIGSAIERAFAANAPYLVEVEISGKP"
BASE COUNT          767 a         1435 c         1498 g          857 t
ORIGIN      
        1 catcgtgcgg cgctcgttcc tcagagcgtc aaaaagctcg taggtgccgg gttcgaagta
       61 gtcatagaag ccgggacggg agtccctgcg ggttatcccg acgccgccta cgtcgatgcc
      121 ggtgcgacca tcgggggcga tcgggcgggc ctgctcgcct cggcggacgt cgtcctgcgg
      181 gtgcgcaagc cggaagcgga cgagatcgcc gggctcaaaa agggatcggt gcacgtcagc
      241 tttctggatc ccttcaacga gagagggctg gtcgatgcca tggcggccca gggcgtcacg
      301 gccatttcca tggagatgat cccgcgcagc acgcgcgcgc agaagatgga cgccctgtca
      361 tcccaggcca acctcgccgg atacgtcacg gtggttctcg ccgccaatca ttgccccaag
      421 atctttccca tgatgatgac gccggccggg acgattgccc cgtcgcgggt gttcgtgatc
      481 ggcgcggggg tcgccggact gcaggcgatc gccacggcga agcggctcgg cgctagagtc
      541 gaagcattcg atacccgccc ggtggtggcc gagcaggttc gctctctcgg cggcaagttc
      601 gtcgagatcg atcttggcga ggtcgggcag acagagcagg gctatgcaaa agagctgacg
      661 cctgagcagg tggagctcca gaaagaaggt cagaaaaagg tgatcggcca gtccgacgtg
      721 gtcatcacca ccgcgcaggt gtttggtcgt ccggcacccc gcatcgtgtc aaaggacatg
      781 gtcgaggcca tgcagccggg aagcgtcgtg gtcgacatgg ccgtcgagac cggcggcaac
      841 gtcgagggct ctgttctcaa cgaagtggtc gacgtcggcg gcgtgaagat cattggcctg
      901 ggcaatttgc cgtcgcaggt tgcgcggcat gccagtgaca tgtactcgag caatctcttc
      961 aacctgctgg atgaattctg gagccaggag gaaagcaagc tcgacctcga tccggaagac
     1021 gaaatcgttc gggcctgcgt gatcacgcgg gacggatcga tcgtcaacga aaccatcaag
     1081 aacctctagg agtccggaac cgtggaagcc gttttcctcg ccttcgtatt gatgctctcc
     1141 atcttcctgg gcttcgagct catttccaaa gtgcctgcga cgctgcacac gcctctgatg
     1201 tcgggcgcga acgcgatctc gggcatcacc ctcgtcggcg cgatcacatc cgcgggcgcg
     1261 ggggccgacc agatcgcgac gctgctgggc gctgtcgccg tcggttttgc caccatcaac
     1321 gtggtgggcg gttacctcgt caccaatcgc atgttgtcca tgttcaagag gaaagacacc
     1381 ggtggtcagt cggggggtcg gtcatgagcc ttgaactgat caacctctgg tacatcgtcg
     1441 ccgccgcgct tttcatattc ggcctcaagc agctcagctc gccggacacg gccgtgcgtg
     1501 gcaacctgct gtcatccgtg ggcatgctca ttgccgtggt ggtgaccctg ttctcccggg
     1561 atatcctcga gttccagtgg attgcggcgg ctgctgccgt tggcgcgatc atcggggccg
     1621 ttgccgcgca gagagtggcc atgacgagca tgccggagat ggtcgccctg tttaacggct
     1681 cgggaggcat tgccagcctg ctggttggct gggcggccct ctacctcggc gatggcgccg
     1741 ccgccgaggc cgcagtggcc gacaccgccg tcgtcacggt gggtctttct acctttacca
     1801 tcatcaccat cctgctctcc gtgctgattg gcggcttgac cttctcgggc agcctcatcg
     1861 cctggggcaa gctgtcagag gtgatgacga gccgggccat cgtcttcggg gctcagcgcc
     1921 tggtcaacgc cgctctgctg atcggtctcg ttgcctgtgg cgtgatgttc gccatggagc
     1981 cgtcgctgac ctcaccctat ctctacgcgg taatcgcgct gtcgctcatc ttcggcgtaa
     2041 tggcggtcat tcccatcggt ggcgccgata tgccggtggt gatctcgctg ctcaacagct
     2101 actccggtct tgccgcatgc gcggcgggat ttgcgatcaa caacaacatc ctcatcgtcg
     2161 cgggttcgct ggtgggcgct gcgggcatca tcctgaccaa catcatgtgc aaggccatga
     2221 accgttccct tgccaacgtg cttttctccg gcttcggtgc cgtcaagacc gccaccaagg
     2281 tcgagggcga ggtcaagccg atcacgccgg acgacgcgtt cctcattctg gaagcagcct
     2341 catcggttac gttcgtcccg ggttacggaa tggccgttgc ccaggcgcag cacgtggttc
     2401 gggagcttgg tgagcttctc gaggaaaacg gtgccgaggt gacctacgcc atccatccgg
     2461 tggccgggcg gatgccgggg cacatgaacg tcctgctggc cgaggcgaac gtgccttacg
     2521 atcagctggt cgagatggat gacatcaacc cccgcatcga caacatcgac gttgcggtcg
     2581 tgatcggcgc caacgacgtg gtcaatccgg ctgcccggga ggacgagaac agcccgatct
     2641 acggcatgcc catcatcaac gtcgaccagg cgcgaaccgt gttcgtcctc aagcgatcga
     2701 tggcttccgg gttctcgggc gtggacaacc cgctgttctt cggcgagaac acgcggatgc
     2761 tgttcggcga cgccaaggcg tcgatctcct cggtgatcgc cgagttcaaa gggtagccga
     2821 caggcctttc agggcgtaga cacgcccgac accgccgaga tgaacccatc gaggttgggc
     2881 atcgtgatac ccggcagcgt ggtccggatc gcgttgcact ggagggttct ctgccaacgc
     2941 tagggctttc cggagatctc gacctccacc agatagggcg cgtttgcggc gaacgcgcgc
     3001 tcgatggcgg agccgatctt ctccggccgt gtgaccagct ctccctccat tcccattccc
     3061 tcggccatgg cgggaaaacc cagtgccggt ctggtgagat ccatgtgcgg gtacggcttg
     3121 ttggaccctg catcgaagcg ttggcggtaa atgtcgagat tgtgtttgag aatccgatat
     3181 tcacggttcg acaggatcac gaacacgatg ggcagcccgt gatgcaccgc ggaccacagg
     3241 gcctgaatgc tgtacatggc agagccgtct ccggagatcg ccaggacgcg ccgattgggc
     3301 tgagccacct gaacgcccag ggcgccggcc aggccctggc cgataccgcc gcccgagccc
     3361 gcgaagaagt cgccgggggc ggcgtaatcg aatcgcgatg ccacttccaa cgatgcggtg
     3421 atggattcgt ccacgacgat ggcgcctttg ggcagcgcgc ggctgatttc cagcaaggcc
     3481 ctggctggcg tcatcggcga ggtatcgccc tggcgctcga tccgatccgc ggcggcatcg
     3541 ccggcggccc gatgggattc ggccagcgcg tcgtttcgcg cctttgccgc cgccccgaag
     3601 tcgtcgggcg cgccctcggc gatttcctcc accagatgct cgatcgttgt ccgcaggttg
     3661 ccgaccagac cgagatcggg ggccaggttc agtgccagac gggcatgact ggactccacg
     3721 tgaacgaccc gggcctcctc gggcaacgcg ttttccgcat cgaaccagat ctcctcgaag
     3781 aacgggccgc ccatcagcag aatcagatca tggccggcga gcgcgcggcg aatggcgccg
     3841 gcttcgaacg gcaggcgtcc cttgctgttg gggtgcacgt tcgggaagga aatctgagcc
     3901 cgcaaaccct gctgccatac ggcggccccg gtctgcacgg cgagtgtgat cagcgcccgg
     3961 tgggcaccct cggcggcgat gtcgtcgccg acgacgatgg ctgggtttgc gctcccggcc
     4021 agccagcgcg ccagtcggcg gatgccctcg gcgtccgcga gcgtcccgta gcggagatcc
     4081 cctgcggtag tggcgccaac gctggtttcc tgctccatga cgtccaccgg cagggacatg
     4141 aaaaccgggc cccgcggcgg ctcgttcgcg atcttgaacg cccgccgaag catggggccg
     4201 atctcgtcgg cgctgcgtgg ctcggcgctc cacttgcaga ccggctcggc catggcgacg
     4261 aggtcgtgag cgagcagagg ctcccgcatg cgcatgcggg tgtcctgctg ccccgcggtg
     4321 atgatgagcg gcgagttggc tttcagcgcg ccgtagatca tcccgatgcc gttgccgagc
     4381 ccgggagcga cgtgcaggtt cacgatgccc gtcttgccgc tcgcctgggc gtagaagctt
     4441 gcggcgccca ccgcaacgcc ctcgtgcagg gcgacgtagt agcgcagccc cgggtagtcg
     4501 atgagccggt cgagcagaga attttcggtg gtgccggggt tcccgaagat gcagtcc
//
LOCUS       DBNHKV010000439         6185 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_347358, whole genome shotgun sequence.
ACCESSION   DBNHKV010000439 DBNHKV010000000
VERSION     DBNHKV010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_347358"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1296)
                     /locus_tag="AC16PP_14350"
     CDS             complement(124..1296)
                     /locus_tag="AC16PP_14350"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR6482857.1"
                     /translation="MSRADDIRRHLQAEEPEMLELLTALVTLESPSDVPETQGPVLDE
                     LQRAFDGLGYRTIRVPGRASGGHLYARPGRRRSGGNRRWQLLLGHCDTVWPVGTLEKM
                     PLAADGNHLKGPGIYDMKAGLVEMVFALKALEALGLEPEVEPVIFINSDEEIGSREST
                     RYIRALSQRADRALVMEPSLGPRGRLKTARKGVGRFTVTVRGKAAHAGLDPTAGASAI
                     LELSHVIQKLFALNDPERGASVNVGVIDGGVRANVVAPESSAVVDVRVASQEDAERIE
                     RAILSLEAETPGVSLGVVGRIGRPPLEHTPANRQLWRVATDLACELGIQLEEAMAGGG
                     SDGNTTSLFTATLDGLGAVGDGAHADHEHIQVDQLSQRCALLAMLLLAPPLNSGTG"
     gene            complement(1301..1906)
                     /locus_tag="AC16PP_14355"
     CDS             complement(1301..1906)
                     /locus_tag="AC16PP_14355"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR6482858.1"
                     /translation="MAGDARATIALDVSESIWERFFCVFPLVVVGTVEPDGTYDLAPK
                     HLAMPMSWKNHFGFVCTPRHRTYQNIERTGEFTVTYARPSQAVLSSLAATPRCEDGSK
                     PITAALPTRPAQTVAGVFLDDGYLFLECRRYRVYDDFGTNSLITGEIVAAGVAEDALR
                     SADEDDQDVIRRAPLLSFLYPGRIAEISASQQLPLPAGFSR"
     gene            2070..2273
                     /locus_tag="AC16PP_14360"
     CDS             2070..2273
                     /locus_tag="AC16PP_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482859.1"
                     /translation="MSAGTNQLAIEYKGRQYSGVYSVSGNLMIARIPGISSRSLETAE
                     ADESQARTLLMEILKEADTLGQL"
     gene            2337..3701
                     /locus_tag="AC16PP_14365"
     CDS             2337..3701
                     /locus_tag="AC16PP_14365"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6482860.1"
                     /translation="MSQKRLSRSTLLYFGLPDYAIYLAAIPVSLYLPFVYSRDLGLDL
                     TDIGLILMLARISDVITDPLIGYLSDRTQTRFGKRKPWLAGGALVMMVSAWQLFAPMG
                     PVTNWHLFIWSMLLWLGWTMINIPYFAWGAELSDDYNERTRITGWRQAFGYLGNVSVL
                     AIPTLSGELFGYGSLPREGLTIVGFMALVLLPVLMALALWKVPEPEKHRATEANLLVS
                     SRQMFSNGSFMLLFTGFLMLSMGTTWASSMFMLFATFVVDVEGYTQAILLGFYFVQLL
                     SLPLWLQISYRIGKKETWLLGAAIYAIASPGYLMLGSGDVVGLGIVLGLYGIASGNFT
                     AISLSMKADVIDVAALRTGNHVAGSYMAVWSLGQKLFQAIAVGLALPAVAYFGFDPKG
                     ENGPDELLALRLNITVVPMLLYAASVFVIWRYPLTADRLHQLREAFSRRRARREIKQG
                     STGA"
     gene            3724..4188
                     /locus_tag="AC16PP_14370"
     CDS             3724..4188
                     /locus_tag="AC16PP_14370"
                     /inference="COORDINATES: protein motif:HMM:NF017771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HIR6482861.1"
                     /translation="MSSDRIETLERQIFDLTRELHELRKANVGDEVPNYTFATLTGEA
                     SLLDLFGSRDTLLAIHNMGQGCRYCTLWADGLNSLLPHLESAMAVVVLSKDAPETQRL
                     FANSRGWQFRLASHGGGNYMAGRSVLLDLERVCARRAGRIELDAPVQLLAAA"
     gene            complement(4248..4544)
                     /locus_tag="AC16PP_14375"
     CDS             complement(4248..4544)
                     /locus_tag="AC16PP_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482862.1"
                     /translation="MFGDGSVRIIFTPGHTPGHQTLLISLEKTGPVMLSGDLYHFRAN
                     RRLRRPPNFNVDQEQTLQSMDRLEAVLEETGATLWIEHDKALAETLRKAPAFYD"
     gene            complement(4599..5057)
                     /locus_tag="AC16PP_14380"
     CDS             complement(4599..5057)
                     /locus_tag="AC16PP_14380"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6482863.1"
                     /translation="MKRVLVFLLIGFSTPAFAGPRLYVFDCGLLSLDSVEIFNLAEDE
                     TSVRELFVPCYLIEHENGRMLWDGGLPLSYAQAEGPVAIDGGSVSYERSVVDQLADMD
                     ITPEDVDLVAYSHLHWDHAGTANTFIASEVLMQRAEWAAAMGEGGEFVDT"
     gene            complement(5126..5440)
                     /locus_tag="AC16PP_14385"
     CDS             complement(5126..5440)
                     /locus_tag="AC16PP_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482864.1"
                     /translation="MTIKSAIYDLLVSRRYDAELETVTEDARRRCVEMLAPRPGGRCA
                     MMDTYWPKPPLRLWLMSWSYAADPKRPGFELLRATCDDFSLEHFPPDVEHGFYLASGT
                     KR"
     gene            complement(5453..>6185)
                     /locus_tag="AC16PP_14390"
     CDS             complement(5453..>6185)
                     /locus_tag="AC16PP_14390"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6482865.1"
                     /translation="FRGRAQHYVFTSSMAVYKRSFVQPLKEDFRRHAADDDDPRKAYG
                     VGKVRCEDLLLAEWQRDAFPATWLRVGHTMGPRSPLPTRDPAFFARLEAGRPILVPGE
                     GFAVLQLVHINDVARCMASLLGNERVRGEGYNVSGSEVASVLGVVHLIGRAMGVQPRV
                     VNVPMEVARAHRPPLLHWGESVSGSAMLSIDKALAHLDWEPRFGIEAGYRDAYEWWQR
                     EGRGLFEFDFAPEDALVERLGLATE"
BASE COUNT         1080 a         2050 c         1970 g         1085 t
ORIGIN      
        1 tccccgacca cgtagtcgcc ggtcgcccag gtgtctttcg ggaccggcgc cacgtcgaaa
       61 gcgccctcgg caaggtcgca gatgcgggta acggatgtca gcaggtgtcg cacgcgccga
      121 tcctcaaccg gttccggagt tcaggggagg ggcaagcagg agcatggcca gcagcgcgca
      181 gcgttgggac agctggtcta cctgaatgtg ctcgtgatcc gcgtgggcgc catcgcccac
      241 cgcgcccaga ccgtccaggg tggcggtaaa cagactggtg gtgtttccgt ccgagccccc
      301 gccggccatg gcttcttcca gctgaatgcc gagctcgcat gccagatccg tggccacgcg
      361 ccacaactga cggttcgccg gcgtgtgctc cagcggtggc cggccgatgc ggccgaccac
      421 gccgagagac acgcccggcg tttccgcttc gaggctgagt atcgcccgct cgatgcgctc
      481 cgcatcttcc tggctggcca ccctgacatc caccacggcg ctgctttccg gcgccaccac
      541 gttggcgcgc acgccaccgt cgatcacgcc gacgttgacg gatgcccccc gctcggggtc
      601 gttcagggca aagagcttct gaatcacgtg ggagagctcg aggatggcgc tagcgccagc
      661 cgttggatcc agacccgcat gagccgcttt gccgcgaacc gtaacggtga atcggccaac
      721 gcccttgcgc gccgtcttca gccggccccg gggccccagg gaaggctcca tgaccagcgc
      781 ccggtcggcc cgctgcgaga gcgcgcggat gtagcgcgtg gactcccggc tgccgatctc
      841 ctcatcggag ttgatgaaaa tcaccggctc cacttcgggc tcgaggccca gcgcctcgag
      901 ggctttgagc gcgaagacca tctcaaccag gcccgccttc atgtcgtaga tgcccggacc
      961 tttcaggtgg ttcccatccg cagccagggg cattttctcc agggtcccta ccggccagac
     1021 cgtgtcgcag tggccaagga gcagttgcca tctccggttg ccgcccgatc ggcggcggcc
     1081 cggccgagca tagagatggc cgcccgaggc gcgccccggc acccggatcg tccggtagcc
     1141 gagaccgtcg aaagcccgct gcagctcatc gagcacgggc ccctgagtct cggggacatc
     1201 ggacggtgac tccagggtga ccagagcggt tagcagctcc agcatctccg gctcttccgc
     1261 ctggaggtgg cgacgaatgt catcggcccg gctcatccgg ctacctggag aatcccgcgg
     1321 gcaggggcag ctgctggctg gccgagatct cggcaatccg ccccggatag aggaagctga
     1381 gcaacggcgc ccggcggatc acgtcctgat cgtcctcgtc ggcgctgcgc agagcatcct
     1441 ccgccacgcc agcggcaacg atctcgccgg ttattaggct gttggtgccg aagtcgtcat
     1501 agacgcgata gcgccgacat tcgagaaaga ggtaaccgtc atcgagaaag acgccggcaa
     1561 ccgtctgcgc tggccgcgtc ggcaacgcgg cggtgatggg tttgctgcca tcctcacatc
     1621 gcggggtggc cgcgaggctc gaaagcaccg cctgagaagg ccgcgcgtag gtcacggtga
     1681 attcgcccgt ccgctcgatg ttctgatacg tccggtggcg cggggtgcag acgaagccga
     1741 agtggttctt ccagctcatc ggcatggcca ggtgcttggg cgccaggtca taggttccgt
     1801 ccggctcgac cgtgccaacc accaccagag gaaacacgca gaagaaccgc tcccagatgg
     1861 attcgctgac atcgagcgca atggtcgctc tcgcatcgcc cgccatattg gccctcttct
     1921 caccaggaaa gcgtcccttc cactatcggc agtcgcccca ggccggacca tacggggttt
     1981 ccgagacgcg cccgggccct tcagtgttcg cccaatcgtg ttatctttca gtgcaaatcc
     2041 gacaaacaac gtgacccggt ggggaggcta tgagcgcagg gaccaaccag ctggctatcg
     2101 agtacaaagg caggcagtac tcgggcgtat attccgtgtc cggcaacctg atgattgccc
     2161 ggataccggg aatcagctcc agaagcctgg agacggcgga agccgacgag tcccaggccc
     2221 gcacgctgct gatggaaatc ctcaaggaag cggacaccct ggggcagctc tgagcgccgc
     2281 cgacctggcg ggctgcctga cggtggctga cacgcccgtt taggctcggg ggtctcatgt
     2341 cgcaaaaacg cctttcgcgt tccacccttc tctacttcgg gctgcccgac tacgccatct
     2401 atctggcggc gatacccgtc tcgctctacc tgcccttcgt ctactcgcgg gacctgggcc
     2461 tggacctgac ggacatcggc ctgatcctca tgctggcgcg catcagcgac gtgatcaccg
     2521 acccgctgat cggctatctc agcgatcgca cccagacccg gttcggcaaa cgcaagccgt
     2581 ggctcgccgg cggcgccctc gtcatgatgg tttccgcctg gcaactgttc gctcccatgg
     2641 ggcccgtcac caactggcac ctcttcatct ggtccatgct gctgtggctg ggctggacca
     2701 tgatcaacat tccatacttc gcctggggcg ccgagctctc cgacgactac aacgaacgta
     2761 cgcgcataac gggctggcgc caggcattcg gctacctcgg caacgtgagc gtgctggcga
     2821 ttcccaccct ctccggggag cttttcggct acggttccct gccccgggaa ggtttgacca
     2881 tcgtcggctt catggccctg gtgctgctgc cggttctgat ggccctggcg ctgtggaaag
     2941 tgccggagcc ggagaagcac cgtgccaccg aggccaacct gctcgtgagc agccgacaga
     3001 tgttctcgaa cggttctttc atgctgctgt tcacgggttt tctgatgctg tccatgggca
     3061 ccacctgggc gtcatccatg ttcatgctgt tcgccacgtt cgtggtggac gtggaggggt
     3121 acacccaggc catcctgctg ggcttctact tcgtgcagct gctctccctg ccgctgtggc
     3181 tgcagatttc ctaccggatc ggaaagaagg agacctggct gctcggcgcc gccatctatg
     3241 ccattgccag tcccggatac ctgatgctcg gctccggcga cgtggtcggc ctgggcatcg
     3301 tcctggggct gtacggcatc gcaagcggca acttcaccgc catctccctg tccatgaagg
     3361 cggatgtcat cgacgtcgcg gccctgcgaa cgggcaatca cgtggcgggt tcctacatgg
     3421 cggtctggtc gctgggccaa aagctgttcc aggccatcgc cgtgggcctg gcgttgcccg
     3481 ccgtcgccta cttcggcttc gaccccaagg gcgagaacgg cccggacgag cttctcgcgc
     3541 tgcggctgaa catcacggtg gtacccatgc tgctctacgc cgcctcggtg ttcgtcatct
     3601 ggcgctatcc gctgaccgcc gaccggcttc accagctgcg cgaggctttt tccaggcggc
     3661 gggccaggcg ggagatcaag cagggttcca caggtgcctg attctagaat cgaggaggaa
     3721 ggaatgagca gtgatcgaat agagacgttg gaacgtcaga tcttcgacct gactcgagag
     3781 cttcacgaat tgcgcaaggc gaacgtgggc gacgaggtgc ccaactacac gttcgccacg
     3841 ctgaccggcg aggcgagtct gctggatctt ttcgggtccc gggacacgct gctcgccatc
     3901 cacaacatgg gccagggatg ccgctattgc accctctggg cggacgggct gaacagcctt
     3961 ctgccacacc tggagtcggc catggcggtg gtggtgctgt ccaaggacgc gccggaaacc
     4021 cagcgccttt tcgccaattc acggggctgg cagttccgtc ttgcttccca tggaggcgga
     4081 aactacatgg ccgggcgatc tgtactgctc gatctggaac gtgtttgcgc tcgccgggct
     4141 ggacgaatcg agctggacgc cccagttcag ctactggcgg cggcctgagc agctcgacga
     4201 cggcggcgcg aacatcctgg actgaaccgg cgccacccgg cgcgggctca atcgtagaag
     4261 gcgggcgctt tgcgaagggt ctccgccagg gctttatcgt gctcgatcca aagcgtggca
     4321 ccggtctcct cgaggactgc ctcgagccgg tccatggact ggagcgtctg ctcctggtcg
     4381 acgttgaagt tcggcggccg ccgcaggcgc ctgttggcac gaaagtgata cagatcgcca
     4441 gaaagcatca ccggacccgt cttctccaaa gagatgagca gcgtctgatg gcccggcgtg
     4501 tggcccggcg tgaagatgat cctgacgctg ccgtcgccga acacgtcgtg gtcgccctcg
     4561 agaaacgtga cctcggcatc gaccagcttg tcgaagagct aagtgtcgac gaactcgccg
     4621 ccctcaccca tggccgccgc ccactcggcc cgctgcatca ggacctccga ggcaatgaag
     4681 gtgttggccg tccccgcatg gtcccaatgc aggtgcgagt aggcgacgag gtcgacgtcc
     4741 tccggggtga tgtccatgtc cgccagctga tcgactacgg atcgttcgta gctcaccgat
     4801 cccccatcga tggcgacggg cccctcggcc tgggcgtagg acagcggcaa gccgccgtcc
     4861 cagagcatac gcccgttctc atgctcaatg aggtagcacg gcacgaacag ttcgcgcacg
     4921 gacgtttcgt cctcggcgag gttgaagatc tccacgctgt cgaggctaag taacccgcag
     4981 tcgaagacgt agagccgcgg accagcgaaa gccggcgtcg agaaaccaat cagcagaaag
     5041 acgagcactc tcttcacttt gggacgagtc tcaggaacgg gagcgacaac cataccatca
     5101 gagcagccgg tgccatgggc cggagtcagc gcttggtgcc actggccaag taaaagccgt
     5161 gctcgacgtc gggcggaaaa tgctcgagag agaaatcgtc gcaggtcgcg cggaggagct
     5221 cgaagcccgg ccgtttcgga tccgccgcgt agctccagga catgagccag agtctgagag
     5281 gaggcttcgg ccagtaggtg tccatcatcg cgcaacgccc gccggggcgt ggcgcgagca
     5341 tctccacgca gcggcggcgc gcatcctcgg tcaccgtctc cagctccgcg tcgtagcgcc
     5401 gggagacgag cagatcgtag atggccgact tgatggtcac gctgatcccg tctcactcag
     5461 ttgccaggcc gaggcgctcg accaacgcgt cttccggggc aaagtcgaac tcgaacagac
     5521 cgcggccttc ccgttgccac cactcgtaag catcgcggta tccggcctcg atgccgaacc
     5581 ggggctccca gtcgaggtga gcgagggcct tgtcgatgga gagcatggcc gatccggaca
     5641 ccgactcgcc ccagtgaagg agcggcggac ggtgagcgcg cgccacctcc atgggcacgt
     5701 tgacgacacg cggctgcacg cccatggccc gtccgatcag gtgcacaacg ccgagcacgc
     5761 tggccacctc gctcccggat acgttgtagc cctcacccct gacccgctcg ttgcccagca
     5821 gggacgccat gcagcgcgcc acgtcgttga tgtgcacgag ctgcaggacg gcgaacccct
     5881 cgccgggtac gaggatcggg cgtccggctt ccagacgcgc aaagaacgca gggtccctcg
     5941 tgggcagcgg gctgcgcggc cccatggtat ggccgacgcg cagccacgtc gcggggaagg
     6001 cgtcgcgctg ccactcggcg agcagcaggt cctcgcacct gaccttgccg accccgtaag
     6061 cctttcgggg atcgtcgtcg tctgccgcgt gccgacggaa gtcctccttg agcggctgga
     6121 cgaagctgcg cttgtacacg gccatggagc tggtgaagac gtagtgttga gcccgaccgc
     6181 ggaag
//
LOCUS       DBNHKV010000440         2542 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1764655, whole genome shotgun sequence.
ACCESSION   DBNHKV010000440 DBNHKV010000000
VERSION     DBNHKV010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1764655"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1140
                     /gene="pdxA"
                     /locus_tag="AC16PP_14395"
     CDS             133..1140
                     /gene="pdxA"
                     /locus_tag="AC16PP_14395"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR6482866.1"
                     /translation="MTVGSDDDRPIAMTLGDPAGIGPEVAARLIAGGSLEGKLLLVGS
                     AWALGKGAEAAGVRLPDVEVVDDAGAIRGQVGLIDLGGEPESFRFGEVNAACGALAVR
                     AVEGAARLCLAGKVAAMVTCPINKAAVHAAGYTDDIGHQEILARLTGASWTATMLMTP
                     GLKVVHLSTHKSLIEAARFVTRRNVEEKLALLDETLGRWGLRNPRIAVAALNPHGGEQ
                     GLLGREELDEIEPAVAAARTAGIDAVGPLPADSVFNRAIDGEFDVVLAMYHDQGHIAI
                     KVHNFHESTTATLGIPFVRTSVDHGTAFDIAGSGRADERGLAAALDAARALSQGQLAR
                     L"
     gene            1212..2294
                     /locus_tag="AC16PP_14400"
     CDS             1212..2294
                     /locus_tag="AC16PP_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HIR6482867.1"
                     /translation="MKAAVFREINQPMEIEEVALSKPGPREVLVRTAAAGVCHSDLHF
                     FNGTYPGLLPMVLGHESAGVVEAVGSDVHYVKPGDHVITCLSVFCGHCEFCLTGHLSL
                     CQEPETRRAPDQEPRISHDGAPVQQFANLGSFAEMMLVHEHAVAKIREDMPLDRAALI
                     GCGVTTGVGAVIHTAKVEPGSTVAVIGCGGVGLSCINGAAIAGASRIIAVDMVASKLD
                     LARKFGATDVVNAAEMDAINEVKALTGGGVHYSFEAIGLKATAEQAFGMLRNGGTATV
                     IGMIPPGDMVSLHGPDFLFEKKIQGSMMGSNRFRVDMPRFVDLYLQGKLHLDDLVSSR
                     IRLEDVNDAMAQLETGEIARNVIVFD"
     gene            2303..>2542
                     /locus_tag="AC16PP_14405"
     CDS             2303..>2542
                     /locus_tag="AC16PP_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6482868.1"
                     /translation="MRWLTFLRDGRATFGYVTSNGQGVVDVGARSETADLRSAIAAGA
                     LADLAGSCGDEADVPLDGLTYLPTIPNPDKILCVGL"
BASE COUNT          416 a          803 c          894 g          429 t
ORIGIN      
        1 cacggcgcgc acacggcgta gacctggaaa ctgcttgcaa tgtcatctag cgtgagcacg
       61 gtccgggcgt cgacgttcta atgaccatga gtactgtata tatatacagt attttccccg
      121 gggagggatg ccatgaccgt gggatctgat gatgatcggc caatcgccat gaccctcggc
      181 gacccggcgg gtatcggtcc cgaggttgcc gccaggctga ttgcgggcgg gtcgttggag
      241 ggaaagctcc tcctggtcgg gagcgcctgg gcgcttggca agggcgccga agcggcgggc
      301 gtgaggctcc ccgacgtcga ggtcgtggac gacgcgggcg ccatccgggg tcaggtcgga
      361 ctcatcgatc ttggcggcga acccgaatcc ttccgcttcg gagaggtcaa cgcggcctgc
      421 ggcgcccttg ccgtgcgcgc ggtcgaaggc gccgcccggc tctgtctggc cggcaaggtg
      481 gccgccatgg tgacctgtcc gatcaacaag gcggccgtgc acgcggcggg gtacacggac
      541 gacatcggcc atcaggagat tctggcgcgg ctgacggggg catcgtggac ggccacgatg
      601 ctcatgacgc cggggctcaa ggtcgtgcat ctgtccaccc ataaatctct catcgaggcc
      661 gcccgcttcg ttacccggcg caacgtggag gagaagctcg cccttctcga tgagactctg
      721 gggcgctggg ggctgcgtaa tccgcgcatc gcggtggcag cgctcaaccc tcacggcggc
      781 gagcagggtc tgctgggccg cgaggagctc gacgagatcg agcccgccgt ggcggcggcc
      841 agaaccgccg gtatcgacgc cgtggggccg ctgccggcgg attccgtgtt caaccgggcc
      901 atcgacgggg agttcgacgt ggtcctggcc atgtatcacg atcagggtca catcgcgatc
      961 aaggtgcaca actttcacga gagcaccacg gcgaccctgg gcatcccctt cgtgcgaacc
     1021 tcggtggatc acggaacggc cttcgacatt gccggctcgg gccgggccga cgagcgcggc
     1081 ctggcggcgg cgctggatgc ggcgagggcg ttgagccagg gtcagcttgc gcggctctag
     1141 ggccgtgggg caaactcgcg aggcttaatc gaagcggcgt cgcgtccgcg tcacacagca
     1201 ggggaggcag catgaaggcg gcagtatttc gcgagatcaa ccagcccatg gagatcgagg
     1261 aggtggcgct atccaaaccc ggcccgcggg aggtcctcgt gcgcaccgcc gcggcgggcg
     1321 tctgccacag cgacctgcac tttttcaacg ggacctatcc cggcctcttg ccgatggtgc
     1381 tgggccacga gagcgccggt gtggtggagg cggtgggctc ggacgttcac tacgtgaagc
     1441 ccggcgacca cgtcattacc tgcctttcgg tcttctgcgg gcattgcgag ttctgcctca
     1501 ccggtcatct gtccctgtgc caggagcccg agacccggcg cgctccggat caggaaccgc
     1561 gcatcagcca cgacggggcg ccggtgcagc agttcgccaa cctgggctcg ttcgcggaga
     1621 tgatgctcgt ccacgagcac gccgtggcga agatccgcga ggacatgccg ctggaccgtg
     1681 ccgcgctcat cggctgcggg gtgaccaccg gtgtcggggc cgtcattcac accgccaaag
     1741 tggagccggg ctccacggtg gccgtcatcg gctgcggcgg ggtgggattg tcctgcatca
     1801 acggcgcggc catcgcgggc gcgagccgga tcatcgcggt ggacatggtg gcctccaagc
     1861 tggatctggc ccgcaagttc ggtgcgaccg acgtggtcaa cgccgcggag atggatgcca
     1921 tcaacgaggt gaaagcgctc accgggggcg gcgtgcacta ctcctttgaa gcgatcggcc
     1981 tgaaggcaac ggccgagcag gcattcggga tgctgcgcaa cggcgggacg gccaccgtca
     2041 tcgggatgat tcctcccggt gacatggtct ccctgcatgg gcccgatttt ctgttcgaga
     2101 agaagatcca gggctccatg atggggtcca accggttccg ggtggacatg ccgcggttcg
     2161 tggatctcta cctgcagggc aagctgcatc tggacgatct ggtctccagc cgcatccggc
     2221 tggaggacgt caacgacgcc atggcccagc tcgagaccgg cgagatcgcc cgaaacgtga
     2281 tcgtcttcga ctagggggaa ctatgcgctg gctgactttt ctccgcgatg ggcgcgccac
     2341 gttcggctac gtgacctcga acggccaggg ggtggtcgat gtcggcgccc gcagcgaaac
     2401 cgccgatctg agatcagcca tcgcggccgg tgccctggcc gatctggccg gctcctgcgg
     2461 cgacgaagcg gacgtccccc tcgacgggct gacctacctg ccgacgatcc cgaacccgga
     2521 caagattctc tgcgttgggc tg
//
LOCUS       DBNHKV010000441         2348 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1764942, whole genome shotgun sequence.
ACCESSION   DBNHKV010000441 DBNHKV010000000
VERSION     DBNHKV010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1764942"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="AC16PP_14410"
     CDS             complement(<1..723)
                     /locus_tag="AC16PP_14410"
                     /inference="COORDINATES: protein motif:HMM:NF012981.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR6482869.1"
                     /translation="MARASVFDNQEKQAIVRRILVGMREAGCNEVAYLDDGHGIVRTA
                     LDDVREPFIRKPVATTETANALDTINAARALRAEGCDVVLTLGGDGTNRAFALGWPDP
                     PLIPISTGTNNVFPVLVEATIAGAAAGLIASGQISLADVATQHKAIHVTIVGERDDLA
                     LIDAVLSSERFVGARALLAPETLQTALVTRADPAAVGITSIGGLLKPVADAEDGGLLM
                     QLGGTGGSVVNAPIAPGYYRRVR"
     gene            complement(786..1784)
                     /locus_tag="AC16PP_14415"
     CDS             complement(786..1784)
                     /locus_tag="AC16PP_14415"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020300037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR6482870.1"
                     /translation="MAEAQQKPRELPYVSAITDGVRQVLLEQPNSFVAGEDVGGVGGV
                     YGYYQGLQKEFGPERVYDTPISEEGIVGLGVGAAATGCRPIIDLMFMDFLGECMDEVS
                     NQLAKMRYMFGGHATLPVTMLTMAGAGASLAAQHSQSLEAWICHLPGLKVCMPSSPYD
                     AKGLIISAARDDNPVIVVLNKMSLAITGEVPEEPYAIPLGEANIVRPGGNFTLIATGR
                     MVYEAMTAAEKLKDLGIDTEVIDPRTIQPFDTQMVIESVKKTHRVMIVHEAVRFGGIG
                     GEIAAQIQELAFDYLDAPVARVGAPFSPVPFSPALEAVYVPNADRIVAEAKKLLGL"
     gene            complement(1798..>2348)
                     /locus_tag="AC16PP_14420"
     CDS             complement(1798..>2348)
                     /locus_tag="AC16PP_14420"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR6482871.1"
                     /translation="ISFFGDGASNEGAFHEAANMAALYKLPVIFVCENNGYGEYTPQA
                     KHQAIVDVADRAPGYGMPGVIVDGMDAVAVYEAAGEAIARARAGDGPTLLECKTYRFY
                     DHVGVRGMGMNYRTEEEVEQWKQRDPIVLLEQRLAEQDILSKEAAQAVHDQVTREVDA
                     AVKFANESPMPDPSALLEDVYA"
BASE COUNT          355 a          765 c          798 g          430 t
ORIGIN      
        1 ccggacccgc cggtagtagc cgggggcgat gggcgcgttg accacgctgc cgcccgtgcc
       61 gccgagctgc atcagcaggc cgccgtcctc ggcgtcggcc acgggcttca ggaggccgcc
      121 gatggacgtg atgcccaccg ccgccggatc ggcccgggta accagcgccg tctgcagcgt
      181 ctccggcgca aggagcgcac gggcgccgac gaagcgctcg ctgctcagca cggcgtcgat
      241 gagggcgagg tcgtcgcgct cgcccacgat ggtcacgtgt atggccttgt gctgggttgc
      301 cacgtcggcg agcgagatct ggccggaggc gatgagcccc gcagcggcgc ctgcgatggt
      361 ggcctccacc agaaccggaa agacgttgtt ggttcccgtg gagataggaa tcaggggcgg
      421 atccggccag ccgagcgcga aagctcgatt ggttccgtcg ccgccgagcg tgagcaccac
      481 gtcgcaacct tccgctcgca gtgcccgggc cgcgttgatg gtatccaggg cgttggcggt
      541 ttccgtggtg gccaccggct tgcggatgaa gggctcgcgc acgtcgtcca gggccgtgcg
      601 aacgatgccg tggccgtcgt ccagataggc gacctcgttg cagcccgcct cgcgcatgcc
      661 caccaggatg cggcggacga tggcctgctt ttcctggttg tcgaagacgg acgcacgcgc
      721 caccaggcgc cggacatctt tgcccgacgc cgggttcgcg atgatgccta tggtgcccag
      781 ggtcgctata agcccagcag cttcttcgct tcggctacga tgcgatcggc gttgggcacg
      841 taaacggctt ccagcgccgg gctgaacggc accggcgaga agggcgcgcc aacccgcgca
      901 acaggcgcat cgaggtagtc gaacgctagc tcctggatct gcgcggcgat ctcgccgccg
      961 atgccgccga agcgcacggc ctcgtgcacg atcatcaccc gatgggtttt cttgaccgac
     1021 tcgatgacca tctgggtgtc gaatggctga atggtgcgtg gatcgatgac ctcggtgtcg
     1081 attccgagat ccttcagctt ctccgccgcg gtcatggctt cgtagaccat gcgcccggtg
     1141 gcgatgagcg tgaagttccc gcccggcctg acgatgttcg cctccccgag ggggatggcg
     1201 tacggctcct cgggtacctc gcccgtgatg gccagggaca tcttgttcag caccacgatg
     1261 accggattgt cgtcccgcgc tgcagagatg atcagcccct tggcgtcgta ggggctggag
     1321 ggcatgcaga ccttcaggcc cggcaggtgg cagatccacg cctccaggct ctgggaatgc
     1381 tgggctgcca ggctcgcgcc ggcgccggcc atggtgagca tggtgacggg aagcgtcgcg
     1441 tggcctccga acatgtagcg catcttggcg agctggttgc tcacctcgtc catgcactcg
     1501 ccgaggaagt ccatgaacat caggtcgatg atgggacggc agccggtcgc cgcagcgccc
     1561 acgccgagcc cgacgatgcc ttcctcggag atgggtgtgt cgtaaacccg ctcgggtccg
     1621 aactccttct gcaggccctg gtagtagccg taaacgccgc cgaccccgcc gacgtcctcg
     1681 ccggcgacga aagagttggg ttgctccagc agtacctgcc ggacgccatc ggtgatggcg
     1741 gagacgtaag gcagttccct cggtttctgt tgtgcttcag ccatggtttt tctccgctta
     1801 agcgtacacg tcttcgagca gcgcgctcgg gtccggcatg gggctttcgt tggcgaactt
     1861 gaccgcggca tcgacttcac gggttacctg gtcgtgcacg gcttgggcgg cctctttgga
     1921 gagaatgtcc tgttccgcca ggcgctgctc cagcagcacg atgggatcgc gctgcttcca
     1981 ctgctccact tcttcctcgg tgcggtagtt catccccatg ccgcgcacgc cgacgtggtc
     2041 gtagaagcgg taggtcttgc actccagcag ggtggggcca tcaccggcgc gggcccgggc
     2101 gatggcttcg ccggcggcct cgtaaacggc caccgcgtcc atgccgtcca cgatcacgcc
     2161 tggcatcccg tagccgggcg cccggtcggc cacgtccacg atggcttggt gtttggcctg
     2221 aggcgtgtac tcgccgtaac cgttgttctc gcagacgaag atcacgggca gcttgtagag
     2281 cgcggccatg ttggccgcct cgtgaaaggc gccctcgttg ctcgcgccgt cgccgaagaa
     2341 ggagatgg
//
LOCUS       DBNHKV010000442        14008 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_60860, whole genome shotgun sequence.
ACCESSION   DBNHKV010000442 DBNHKV010000000
VERSION     DBNHKV010000442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 14008)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14008)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14008
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_60860"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..772)
                     /locus_tag="AC16PP_14425"
     CDS             complement(14..772)
                     /locus_tag="AC16PP_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6482872.1"
                     /translation="MQYRPLGNTGIEVPLIGLGTVKLGRDQGVRYPRPFTIPDARAAA
                     ALLKKARELGVNLLDTAPAYGTSEERLGALIGAERDHWIICTKVGEEFSNGVSRFDFS
                     PEHTRRSVERSLERLRTDRLDIVLIHSSGADVEILENLGTLDALRELQASGRVRAIGI
                     SHKTPDGGRLALAAGCDVIMTVLNPADREQAPIVAEAGRIGCGVLVKKALASGFAGPE
                     SLEWVAAQPGVSCIVTGTIDPEHLAANVAAVCPA"
     gene            complement(774..1943)
                     /locus_tag="AC16PP_14430"
     CDS             complement(774..1943)
                     /locus_tag="AC16PP_14430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6482873.1"
                     /translation="MSGRDWQTDAIIVGGGIAGLWLANLLVRQGYQVVVLEKDRLGAG
                     QTLASQGMIHGGLKYALTGRLTRASQAIADMPERWRSCLDGTGDLDLSALAPLAERYY
                     LFAESSSLGRLTGFFASRSLRGRIEKVEPHDYPCVFANPAFHGVVYALRDFVLNTGAL
                     VSQLNDTLRGCVFQHAVAPEDLLVEADRVVLRLPDASLTARHLIICAGAGSQPLLDGL
                     GLRRPRMQLRPLHQVVVRHEHPHPMYAHCLTGIRRAEPRLTITSHPDGDHWLWYLGGQ
                     LATDGVKLDAPALIDRARRELEACVPWLSWDDARFETLRIDRAEPEQTRGGRPDEAFA
                     AAVGSCVVCWPTKLSLVPDLGDKVLGLLDPPSAPHPGSPDLGLPTARVGRSPWET"
     gene            2029..3330
                     /gene="waaA"
                     /locus_tag="AC16PP_14435"
     CDS             2029..3330
                     /gene="waaA"
                     /locus_tag="AC16PP_14435"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HIR6482874.1"
                     /translation="MWRILYQVLLPLAYPLVRLRLLWRARREPEYGRRIPERLGRVPA
                     SVPRGVIWFHTVSAGETIAAAPIIDRLLSEFPESDFLVTTMTPTGSQQVIARLGGRVA
                     HCYAPYDFRGGVERFLDAVAPRLLVIFETELWPNLLEAATRRGIPVDLVNARLSERSA
                     RGYARIGALTRRMLGQLQHIACQYPAHAQRFVALGAQPGRVSVLGSVKFDLRLPDDHR
                     ARCAELARRWRLEGRPVWIAGSTHPGEDAPVLAAHDAVRSACPGACLLLVPRHPSRAA
                     DLEKMVQEAGFSVVRQSVAAGAEAGVTVDVVIGDVMGQLLYLYGLSQAAFIGGSLVPV
                     GGHNPIEPALCGQPLLTGPHTFNFPDVVAAFEDSAALLRVGDAAGLAAEVARCLGDAE
                     LRERMAASARRVVAANTGATERLLALLREHVGRVSGGGPLS"
     gene            complement(3323..4711)
                     /locus_tag="AC16PP_14440"
     CDS             complement(3323..4711)
                     /locus_tag="AC16PP_14440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HIR6482875.1"
                     /translation="MNKPRLIIALLGLAWASAASATDILDAYRDAQSNDPVLGAARAG
                     LDATQELIPQSRSALLPRVAIGGQTSWNERSFPSPVFIEDIGTRVRIPDQEFNEHGWQ
                     AELRQPLFDLEAWFNFRSSQAGVEGAEWGFVSVEQDLIVRTVVAYLNVLRAQDLLDSA
                     KAAEDAVKRQLEQVQQRFDVGLVAITDVLEAQAVYDSAQVVRIQAAGDHDIFFETLRT
                     LTGQPYDSIARISEALPVVDPTPGNEEEWVNTALTGNPTILAANSQLDAAERTLKARR
                     SGHMPTVDATVSTNHFVTGGASFLGGKIDQDVYALSITLPIYQGGLTSSRAREARALA
                     EQARQELLDRQLTVSRDTRNLFRAVATDVVRVRARKKAIESSKSALEATQTGYEVGTR
                     NIVDVLDAQNRLYASEFDYADSRYNYIIDLMQLRQTAGTLNGEDVEQLNVYADSNDPV
                     ERLNSLKARVIN"
     gene            4992..5396
                     /locus_tag="AC16PP_14445"
     CDS             4992..5396
                     /locus_tag="AC16PP_14445"
                     /inference="COORDINATES: protein motif:HMM:NF018552.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HIR6482876.1"
                     /translation="MAECDANYLRLMKLFPSLREEDVSDFAVCIGDMHYEVCIEVQDR
                     GPYTTLVRLSQLPEAPWSTRPALTVRVYHDARSAEVVEYQRGGHFQAVYDYPNRDMRQ
                     PDEKAQVNRFLGEFLAMCLTHGVSVREPEFAS"
     gene            5473..6252
                     /locus_tag="AC16PP_14450"
     CDS             5473..6252
                     /locus_tag="AC16PP_14450"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HIR6482877.1"
                     /translation="MTDLHLQPRESDRLLGVDTADSLRAVLARALAEGRPDALLVTGD
                     VSHEGDEASYRRFAAIVADHYEGPVLCLPGNHDKSAAMREVLGETWALDLQEWSLIGL
                     DSHVDGQVGAGLSETDMAALEGRLAASRGRHVLVATHHPPVDVGCPWLDKDRIQNGAE
                     LLEWLSEHAGVKGMVFGHAHQVIESAYENLALLGTPATCFQFAPRTERFAVDERKPGY
                     RWLRLDAQGGLQTEVGRVDDYPLTLDLSERSRSAGRAPKPN"
     gene            6270..8162
                     /gene="parE"
                     /locus_tag="AC16PP_14455"
     CDS             6270..8162
                     /gene="parE"
                     /locus_tag="AC16PP_14455"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HIR6482878.1"
                     /translation="MADASKYSAESLQVLSGLEPVRRRPGMYTDTSRPNHLVQEVVDN
                     SVDEALAGYAREIEVTLFKDGSVEVVDDGRGMPVDVHPKHKVSGVELILTRLHAGGKF
                     DNENYSFSGGLHGVGVSVVNALSEWLEVEVKREGKVFRQRYKGGKPTSKLKPEGTVGQ
                     RNTGTRVTFKPDAGYFDSVNVAVGRLKHLLRAKAVLCPGLRVSLNVEGKESESWHYEN
                     GLEGYLVNALAGAEVIPEKPFMHAATGNQEGVDWAVQWVLDGGELITESYVNLIPTAQ
                     GGTHVNGLRSGLIDALKEFCEFRDLLPRGVKLTGDDLWDQCSYVLSAKLKDPQFSGQT
                     KEKLSSRQSAVFIGGVAKDAFSLWLNEHPQDGERLAELAISNAQKRVQAAKKVARKKV
                     TAGPALPGKLADCSGQDAERAELFLVEGDSAGGSAKQARDREFQAVMPLRGKILNTWE
                     VDVSQVLDSQEVHDIALAIGVDPGSPDLSGLRYAKVCILADADSDGLHIATLLCALFL
                     RHFRALVDAGHVFVAMPPLYRIDVGKEVFYALDEAEKEGVLDRIAAEKKRGQIQVQRF
                     KGLGEMNPMQLRETTMNPDTRRLVRLELDSPTKTDEVMDMLLAKKRSPDRRAWLEKKG
                     DQASLA"
     gene            8202..10454
                     /gene="parC"
                     /locus_tag="AC16PP_14460"
     CDS             8202..10454
                     /gene="parC"
                     /locus_tag="AC16PP_14460"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HIR6482879.1"
                     /translation="MESMETENPGIETIPLRAYTERAYLDYAMYVINDRALPHIADGL
                     KPVQRRIIFAMSELGLSNQAKFTKSARTVGDVLGKFHPHGDSACYEAMVLMAQPFSFR
                     YPLVDGQGNWGAPDDPKSFAAMRYTEARLSAYAELMLSESKQGTVDFIANFDGTLTEP
                     RLLPARAPFVLLNGSTGIAVGMATDIPPHNMNEVISACIHLLDSPKATVADLMTHIKA
                     PDFPSEAIIITPPAEIREAYETGRGSVKARAVWKKEAGDIVVTALPHQASPAKVLEQI
                     AQQMNAKKLPMLADLRDESDHENPTRLVLVPRSNRVDAERLMSHLFATTDLEKSFRIN
                     LNIIGLDQRPQVKSLQAILKEWLTYRLETVRRRLTFRLDRINERLHILDGLLKAYLNL
                     DEVIRIIREDDHPKASLMKRFTLSEIQANAILDLRLRQLAKLEEDKLKAEKSELESEK
                     ADIEKTLKSKARMKALLKSELVEIAEGYGDARRSPLGAVEDAAAYSEEDLISNEPITV
                     VLSEKGWVRSAKGHETNPEELSYRSGDGYGHAARGRTNDILVFVDSTGRCYTVAAHSL
                     PSARGQGEPLTGRLKPPAGSRFVGVALGPGESKVLLASDAGYGFVGRLSGLVAKNKSG
                     KACLSVPKGGLALPPLLLADQPDLQVVAVTDQGRMLVFPLTEVPELTRGKGNKLLNIP
                     SAAFKSGEERLVAVTVIRPDDELLVLAGQRHLRMKHRDLENYVGERARRGRKLPRGFQ
                     KVDSLAVAGR"
     gene            complement(10418..11539)
                     /locus_tag="AC16PP_14465"
     CDS             complement(10418..11539)
                     /locus_tag="AC16PP_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482880.1"
                     /translation="MKLTSWLGLVKPPPLSLVGACLGSLVSVAVMLSYWALNDPDQLR
                     HDAYGRSMSTGLAQLAIEPLLTHDRITLGVIANQAADIPGVSGVEIVTVNEQALASSG
                     SLSNARVYQSPVTFNDQIMGAVRVALSEPPSHLGDAVAVAAVTLIAAPFLALALLSIR
                     LQPPLPRQPARIEVELHTPRERHFLVAVNLYDQLGMEPTQRVAELNYARIAAGKVAGL
                     YGGDVENLPGTGLLLDFNDGGDADRPWQVLSAAAVLSELLDEEAGDYRVAIHVGDAPV
                     GHRVLLDEQAVGDAALLSALAKPATLAASSVFSACLDRGGELVERTVIEHPLLEELAT
                     VGPDSVLITALSAPQQDLVQHQAAHLAGHRPATASESTF"
     gene            11565..12803
                     /gene="serB"
                     /locus_tag="AC16PP_14470"
     CDS             11565..12803
                     /gene="serB"
                     /locus_tag="AC16PP_14470"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HIR6482881.1"
                     /translation="MSSEIVLVNVSGEDQPGLMAMLTSTLARYQVRVLDVGQAVIHDE
                     LNLGMLLQIISDGNGVEAAEVLSAIEANVLDQGMTARFTPISAGDYADWVKSQGKPRY
                     IVTLLTAGATGEQLAAVTSIVRHHGLNIDAIRRLSGRLPLTSDAGGQRICIEMSLRGV
                     LADAGALKAGLLEAAERLAFDFSVQQDTVYRRNRRLVAFDMDSTLIDAEVIDELAAAH
                     GVGEQVARITERAMRGELKFADSFRERAMLLKGLSEDALREVADGVALNDGARRLIRT
                     LKHFGYKTAIISGGFQYVGERLRDELGIDYVYANSLEIRDGLMSGEVEGEIIDAGRKA
                     ELLKEIARREGISGQQTIAIGDGANDLPMLNAAGLGVAYHAKPMVKETASHAISTFGL
                     DSVLYLMGFSDRDIDEALTG"
     gene            complement(12838..>14008)
                     /locus_tag="AC16PP_14475"
     CDS             complement(12838..>14008)
                     /locus_tag="AC16PP_14475"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR6482882.1"
                     /translation="DYAEVQRSHGLAGALEVCRHVERCLGELIASDALHRLADHQFAF
                     AVTADNEDEVRAEADQLRGTIEASLLEVREKTVRPTITLAGMEIATEEDQGVRERLED
                     CLNEAYDVLRTSMEQNEGNCVELKSQGAGDEPESESARLLRLINEAIDDQRFVLLFQP
                     MISLHGDSDEHYEVLLRMLDDEGKQMLPGAFLRTAIENNLAGRIDRWVILQAIKSLSA
                     HRAKGNLTRLTINLTSNSVNDPEFLEWLGVAFKAVRLPTDAVVLQITEQDANTYVRQT
                     REFFEQLKQMHCVASLSRFGRLGNSFELLEQIPAEYVKVDGSLIEKIASSSEAREALT
                     EMIHGLQGAGKLTVVPMVESANILSTLWQAGANYVAGHYLQEPSRSMDYDFSADD"
BASE COUNT         2395 a         4504 c         4702 g         2407 t
ORIGIN      
        1 cgcggggccg gcttcaagcc gggcagacgg cggccacgtt ggcggccagg tgctcgggat
       61 ctatggttcc ggtgacgatg caggacacgc cgggctgtgc cgccacccac tccagtgatt
      121 cgggccccgc gaagccgctt gccagggctt tctttacgag cacgccgcag ccgatgcgtc
      181 cggcctccgc cacgatgggg gcctgctcgc ggtcggccgg gttcagcacc gtcatgatca
      241 cgtcgcagcc ggcggccagg gcaagccggc cgccgtcggg ggttttgtgg gagatcccga
      301 tggctcgcac gcggcccgaa gcctgcagct cccggagcgc atccagcgtg cccaggttct
      361 ccaggatctc cacatccgct ccgctggagt ggatgaggac gatgtccagg cggtccgtgc
      421 gcaggcgctc gagagacctc tcgacgctgc gccgtgtgtg ctcgggcgag aagtcgaaac
      481 gggacacgcc gttcgagaac tcctcgccga ccttcgtgca gatgatccag tggtcccgct
      541 cggcaccgat cagcgcgcca agccgttcct cgctcgtgcc gtaggcgggg gcggtgtcga
      601 ggaggttcac gcccagttcc ctggctttct tgagcagggc ggcggcggcc cgggcgtcgg
      661 gaatggtgaa cgggcgcgga tacctgacgc cctggtcgcg gcccagcttg accgtgccga
      721 gaccgatgag cggcacctcg atgcccgtgt tgccgagggg acggtactgc atgctaggtt
      781 tcccagggtg agcggccgac ccgggccgtg ggcaggccca ggtccggcga gccggggtgg
      841 ggagccgatg gcggatccag cagcccgagc accttgtcgc cgagatcggg taccaggctc
      901 agcttggtgg gccagcacac cacgcagctt ccgacggccg ctgcgaacgc ctcgtcgggc
      961 ctaccccctc gggtctgctc gggctctgcc cggtcgatgc gcagggtctc gaatcgcgca
     1021 tcgtcccacg aaagccaggg gacacaggct tcgagctcgc gccgggcccg gtcgatcagg
     1081 gcgggggcat ctagcttcac gccgtccgtt gccagctgcc cgccgagata ccacagccag
     1141 tgatcgccgt cggggtggct ggtgatggtc aggcgcggct ccgcgcggcg gatcccggtc
     1201 aggcagtgag cgtacatggg atgcggatgc tcatgtcgga ccaccacctg atgcagcggg
     1261 cgcagctgca tgcgcggtct ccgcagcccc aggccgtcga gcagcggctg ggagccggcg
     1321 ccagcgcaga tgatgaggtg tctcgccgtc aacgacgcgt ccgggagccg gagcaccacc
     1381 cgatccgcct cgacgagcag atcctccggg gcgacggcgt gctgaaagac gcatccccgc
     1441 agcgtgtcgt tcagctgcga gacgagggcg ccggtgttga gcacgaagtc gcgcagcgcg
     1501 tagacgacgc catggaaggc ggggtttgcg aaaacgcagg ggtaatcgtg cggctcgacc
     1561 ttctcgatcc gtccgcgcag ggagcggctg gcaaagaacc ccgtcaacct gcccaggctc
     1621 gacgactcgg caaacagata ataccgctcg gccaagggag caagggcgga aagatccagg
     1681 tcgcccgtcc cgtccaggca gcttcgccag cgctcgggca tatcggcaat ggcctgcgag
     1741 gcccgggtga gccggccggt gagcgcatac ttcagcccgc cgtgaatcat gccctggctc
     1801 gccagcgtct ggccggcgcc cagccggtcc ttttcgagta ctaccacctg atatccctgc
     1861 cgcaccagga ggttggccag ccacaggccg gcgatgccgc ccccgacgat gatcgcgtcc
     1921 gtctgccagt ctcgcccgct catgggagcg cgagcctacg gtttcgtccg cagggccgca
     1981 atcccgggca ttcttgacgg ggaccgcgcg cctgcgcagg cttgcgccat gtggcggatc
     2041 ctttatcagg tgctcctccc cctggcctat ccgctggtcc ggttgcggct gctgtggcgc
     2101 gcgcgccgtg agcccgagta cgggcgacgt atccccgagc ggttggggcg ggtgccggca
     2161 tcagtgccca ggggcgtcat ctggttccac accgtctcgg ccggcgagac catcgctgct
     2221 gcgcccatca tcgatcgtct gctaagcgag tttcccgaga gcgactttct ggtcaccacc
     2281 atgacgccaa ccgggtccca gcaggtcatc gcccgcctag gcggacgggt cgctcactgc
     2341 tacgcgccct atgacttccg cggcggcgtg gagcgcttcc tcgacgccgt cgccccccgg
     2401 ctgctggtga tcttcgaaac cgagctgtgg cccaacctcc tggaggcagc tacccggcgg
     2461 ggtattcccg ttgatctggt caacgccagg ctgtctgaac gatccgcgcg cggctatgcc
     2521 cgaatcgggg ccctgacgcg cagaatgctc gggcagctgc agcacatcgc ctgccagtac
     2581 ccggcccacg cccagcgatt cgtcgccctt ggcgcccagc ccgggcgggt gtccgtgctc
     2641 ggcagcgtca agtttgacct gcgcctcccc gacgaccacc gcgcccgatg cgccgagctc
     2701 gcccgtcgat ggcggctgga aggccgtccg gtctggatcg cggggagcac tcatcccggt
     2761 gaggatgcgc cggtcctggc ggcgcacgat gcggttcgca gcgcttgtcc tggtgcgtgc
     2821 ctgctgctgg tgccgcggca cccgagccgg gcggcggacc tggagaagat ggttcaggag
     2881 gcgggatttt ccgtggtcag gcagagcgtc gctgccggcg cggaagcagg ggtgacggtg
     2941 gacgtggtga tcggcgacgt catggggcag ctgctctacc tgtacggttt gtcccaggcc
     3001 gctttcatcg gcggcagcct ggtgcctgtg gggggacaca accccattga gccggccctg
     3061 tgcggtcagc ccctgctgac ggggccccac acgttcaatt tccctgacgt ggtggccgcg
     3121 ttcgaggact cggcagctct cttgcgggtc ggcgacgcgg ccggccttgc cgctgaggtc
     3181 gcgcgctgcc tgggcgatgc cgagttgcgg gagaggatgg ccgcgtccgc gcgccgggtc
     3241 gtcgccgcga ataccggagc gaccgagcgg ttgctcgcgc ttctgcgcga gcacgtcggc
     3301 cgcgttagcg gaggcggtcc gctcagttga tgactcgggc tttcagcgag ttgagccgct
     3361 ccacggggtc attggagtcc gcgtacacgt tcagctgctc gacgtcctcc ccgttgaggg
     3421 tgccggccgt ttgcctgagc tgcatgagat cgatgatgta gttgtagcgg gagtcggcgt
     3481 agtcgaactc ggatgcgtag agtcggttct gcgcatccag cacgtcgacg atgttgcggg
     3541 tgcctacctc gtagccggtc tgggtggctt cgagggccga cttgctggac tcgatggcct
     3601 tcttgcgcgc ccggacccgg accacgtcgg tggcgaccgc gcggaacagg ttgcgggtat
     3661 cccggctcac ggtcagctgc cggtccagca gctcctgacg cgcctgctcg gccagagccc
     3721 gagcctcccg cgcacgcgac gaggtcaggc cgccctggta gatgggcagg gtgatggaca
     3781 gcgcgtagac gtcctgatcg attttgcccc cgagaaagga agcaccgccg gtgacgaaat
     3841 ggttggtgct caccgtggca tccaccgtgg gcatgtgccc cgagcgccgc gccttgaggg
     3901 ttcgctccgc ggcgtcgagc tgggagttgg ccgcgaggat ggtcgggttg ccggtcaatg
     3961 cggtgttcac ccactcctcc tcgttgcccg gcgtcgggtc gaccacgggc agcgcttccg
     4021 agatgcgcgc gatgctgtcg taaggctgtc cggtgagggt gcgcagggtt tcgaagaaga
     4081 tgtcgtgatc gcccgcggcc tggatccgga ccacctgggc gctgtcgtag acggcctgtg
     4141 cttccagcac gtctgtgatg gccaccaggc ccacgtcgaa gcgctgttga acctgctcca
     4201 gctggcgctt caccgcgtcc tctgccgctt tcgcggagtc cagcagatcc tgggcccgca
     4261 ggacgttgag gtaggcgacc acggtgcgca cgatcagatc ctgctccacc gacacgaagc
     4321 cccattcggc gccctcgacg ccggcctgag agctgcggaa gttgaaccag gcttccaggt
     4381 cgaaaagggg ctggcgcagc tcggcctgcc agccgtgctc gttgaattcc tgatcgggaa
     4441 tccgtacccg ggtgccaatg tcctctataa agacgggtga cgggaagctc cgctcgttcc
     4501 aggatgtctg cccgccgatg gcgactctgg gcaggagcgc cgaacggctc tggggtatca
     4561 gttcctgggt tgcatccagg ccggcacggg cagcgcccag aacggggtcg ttgctctgtg
     4621 cgtcccggta cgcgtccagg atatcggtag cgctcgcggc agaggcccac gccaggccca
     4681 gcagggcgat gatcaatctg ggtttgttca aggcaatgtc cgtctttgct gtaggaagag
     4741 agcgcgcata ttagcaaacg caccctgcga agcaccgccg agctgatcgt cggacgcttg
     4801 ggcggggcgt tgaactggtt cccatccctg cgccatctgc ccctggaatt gtgggccgtt
     4861 gccttaaatt agccgtcgca gcgctgtcaa cggtgacgcc tccgcgcggc gtcggtatat
     4921 tggcaggtga aatggccggc ttttcgtgga cagtgcccga caaacgttac agcattgatt
     4981 tgcccgcaca catggctgaa tgcgatgcca actatctgcg gctgatgaag ctcttcccct
     5041 ccctgcgcga ggaagacgtg agcgactttg ccgtctgcat cggcgacatg cactacgagg
     5101 tttgcatcga ggtacaggat cgaggcccgt acaccacgct cgtgcggctc agccagctac
     5161 ccgaagcgcc ctggagcacg cggccggcgc tcaccgttcg tgtctatcac gatgcccgca
     5221 gcgcggaggt ggtggagtat cagcgcggcg gccactttca ggcggtctac gattatccga
     5281 acagggacat gcgccagccc gacgagaagg cgcaggtgaa tcgattcctg ggtgagttcc
     5341 tcgccatgtg cctgactcac ggggtttccg tgcgcgaacc ggagttcgcc agctgagcgg
     5401 ctccggaagt cgcacccccc cggagcccga cgtcggccgc gcggccatcg aaacctgaaa
     5461 accgttctcc acattaccga cctgcatctg cagccccggg aaagcgaccg gttgctgggc
     5521 gtggacaccg cggacagcct tcgtgccgtc ctggcaagag ccctggcgga gggccgcccg
     5581 gacgctctgc tggtcacggg cgacgtcagc cacgaagggg atgaggccag ctaccggcgc
     5641 ttcgcggcga tcgtcgccga tcactacgag ggcccggtgc tctgcctgcc cggtaaccat
     5701 gacaaaagcg ctgcgatgcg ggaggtgctg ggcgagacgt gggcgctcga tctgcaggag
     5761 tggtcgctca tcgggctgga cagtcatgtg gacggacagg tgggcgcggg gctttcagag
     5821 acggacatgg cggcgctcga gggccggctt gcggcatcgc gagggcgcca cgtgctcgtc
     5881 gccacgcacc atcctccggt ggacgtggga tgtccctggc tcgataaaga ccgcatccaa
     5941 aacggcgccg agctgctaga atggctgtcc gagcacgccg gtgtaaaagg catggtgttc
     6001 ggccacgcac atcaggtcat cgagtctgcc tacgaaaacc tcgctctgct gggcacgccg
     6061 gccacatgct ttcagtttgc tccgcgcacg gagaggttcg ccgtggacga gcgcaagccg
     6121 ggataccgct ggctgcgcct ggatgcacag ggaggcctgc agacggaggt gggtcgggtc
     6181 gacgattacc cgctcacact cgacctgtcc gagcgcagcc gaagcgctgg ccgggccccc
     6241 aaacccaact gaagaggtcc aatccgttca tggctgacgc cagcaagtat tccgctgaat
     6301 cgctgcaggt gctctccggg ctcgagcccg tgcgccgccg cccggggatg tacaccgata
     6361 cctcccggcc gaaccatctg gtccaggaag tggtggacaa cagcgtggac gaggcgctcg
     6421 ccggatatgc gcgggaaatc gaggtcacgc tgttcaagga tggctccgtg gaggtggtcg
     6481 acgatggccg cggcatgccc gtggacgtcc atccgaagca caaggtttcc ggggtggagc
     6541 tgatcctcac ccgccttcac gccggcggca aattcgacaa cgagaactac agcttctccg
     6601 gcggcctgca tggcgtgggc gtttccgtgg tgaacgctct ctccgaatgg ctggaggtgg
     6661 aagtaaagcg cgaaggcaag gtctttcgcc agcgctacaa gggcggcaag cccaccagca
     6721 agctgaaacc cgaggggacc gtgggtcagc gcaacacggg gacccgggtc acgttcaagc
     6781 ccgatgccgg atacttcgac tccgtcaatg tcgccgttgg caggctcaag catctgctgc
     6841 gggccaaggc cgtgctctgt ccgggtctgc gcgtgagcct aaacgtggaa ggcaaggaat
     6901 ccgagtcctg gcactacgag aacggtctgg aaggttacct ggttaacgcg ctcgccggtg
     6961 ccgaggtaat tcccgagaag ccgttcatgc acgccgccac tggcaaccag gaaggtgtgg
     7021 actgggccgt gcagtgggtg ctcgatggcg gcgagctgat taccgagtcc tacgtcaacc
     7081 tgatccccac ggctcaggga gggacgcacg tcaacggtct gcgttcgggc ctcatcgatg
     7141 cgctcaagga attctgcgag ttccgggatc ttcttcctcg gggggtcaaa ctcaccggcg
     7201 acgatctctg ggatcagtgc agctacgtgc tctccgccaa gctcaaagac ccgcagtttt
     7261 ccgggcagac caaggaaaag ctctcttccc gccagtcggc cgttttcatt ggcggcgtgg
     7321 ccaaggacgc cttcagtctc tggctcaacg agcacccgca ggacggcgag cggctggcgg
     7381 agctggccat ctccaacgcg cagaagcggg tccaggcggc aaagaaggtc gcgcgcaaga
     7441 aggtcactgc gggtcccgct ctgcccggga agctggcgga ctgctccggt caggacgccg
     7501 agcgggccga gctctttctt gtcgaaggag attcggccgg cggctccgcc aagcaggcgc
     7561 gggatcgaga gttccaggcg gtcatgccgc tgcgcggcaa gatactcaat acctgggaag
     7621 tggatgtcag tcaggtcctc gactcccagg aggtgcacga catcgccctg gccatcgggg
     7681 tggatccggg ctcgccggac ctgagcggcc tgcgctacgc gaaggtctgc atcctcgcgg
     7741 acgcggactc ggacggcctg cacattgcta cgcttctctg cgcgctgttt ctgcgccact
     7801 tccgcgcgct ggtcgacgcc ggacacgtgt tcgtcgccat gccgccgctt taccggatcg
     7861 acgtgggcaa ggaagtcttc tacgccctgg acgaggcgga gaaagagggc gtgctggacc
     7921 ggatcgcggc ggagaagaaa cgcgggcaga ttcaggtgca gcgattcaag gggcttggcg
     7981 agatgaaccc gatgcagctg cgcgagacga cgatgaatcc cgacacccgt cgattggtcc
     8041 ggctcgaact cgactcaccg acgaagaccg acgaggtcat ggacatgctt ctggccaaga
     8101 agcgttcgcc cgatcgccgc gcctggctgg agaagaaggg cgaccaggcg agcctcgcgt
     8161 agccttcgaa caaccgcaat ttggaggcgc cagatggcgc catggaaagc atggaaacgg
     8221 aaaatcccgg catagaaacc atacccctgc gggcctatac ggagcgggcg tatctcgact
     8281 acgccatgta tgtcatcaac gatcgcgcgt tgccccacat tgccgacggg ctcaagcccg
     8341 tccagcgccg gatcatcttt gccatgagcg agctgggcct ttcgaatcag gccaagttca
     8401 ccaaatcggc ccggacggtc ggcgacgtgc tgggcaaatt ccatccccac ggcgacagcg
     8461 cgtgttacga agccatggtg ctgatggccc agccgttttc cttccgctac ccgctcgttg
     8521 acggccaggg caactggggc gcgcccgacg accccaagtc tttcgcggcc atgcgctaca
     8581 cggaagcgcg cctgtctgcc tacgccgagc tcatgctttc ggaatccaag cagggcacgg
     8641 tggacttcat tgccaacttc gacggcacgc tcaccgagcc ccggcttctg ccggcccggg
     8701 cgcccttcgt gctgctcaac ggcagcacgg gcatcgcggt gggcatggcc accgacatcc
     8761 cgccgcacaa catgaacgag gtcatcagcg cctgcattca cctgctcgac tcgccgaagg
     8821 ccaccgttgc cgacctcatg acgcacatca aggctccgga cttcccgtcc gaagccatca
     8881 tcatcacccc gccggcagag atccgcgagg cctacgagac cgggcgcgga tcggtgaagg
     8941 cgcgcgcggt gtggaaaaag gaggccgggg acatcgtcgt cacggcgctg ccccatcagg
     9001 cgtcgccggc taaggtgcta gagcagatcg cccagcagat gaacgcgaag aagctgccga
     9061 tgctggcgga tctgcgtgac gagtcggacc acgagaaccc gacccggctg gtcctggtgc
     9121 ctcgttccaa tcgggtggat gccgagcgtc tgatgagcca cctgtttgcc accacggatc
     9181 tggagaaaag tttccggatc aacctgaata tcatcggtct ggaccagcgc cctcaggtga
     9241 aatccttgca ggccattctc aaggagtggt tgacctatcg cctggaaacc gtgcgccggc
     9301 ggctcacgtt tcgtctcgac cggatcaacg agcgactgca catcctcgac ggcctgctca
     9361 aggcctatct gaacctcgac gaggtgatcc ggatcatccg ggaagacgat cacccgaagg
     9421 cttccctgat gaagcggttc acgctgtcgg aaatccaggc caacgccatc ctggaccttc
     9481 gcctgcggca acttgccaag ctggaggagg acaagctcaa ggccgagaag tcggaactcg
     9541 agtcggaaaa agcggacatc gagaagacgc tcaaatcgaa agcccgcatg aaggccctgt
     9601 tgaagtccga actggtcgag atcgccgagg gttacggcga cgctcgccga tcgcctctgg
     9661 gtgcggtgga agatgcggcg gcctacagcg aggaagatct gatcagcaac gagccgatca
     9721 cggtggtgct ctcggagaag ggatgggtcc ggtccgccaa aggccacgaa acaaaccccg
     9781 aggagctgag ctaccggagc ggcgacggct acgggcacgc ggcccggggc cgaaccaacg
     9841 acattctggt ctttgtggac agcacgggcc gctgctatac cgtggcggct cacagcctcc
     9901 cctctgcccg gggacagggc gagccgctca ccgggcggct caaacctccc gcgggatcga
     9961 ggttcgtggg cgtcgccctc ggaccgggcg agtcgaaggt gctgctggca agcgacgctg
    10021 gctacggttt cgtcggccgg ctgtcggggc tcgtcgccaa gaacaagtca ggtaaagctt
    10081 gtctcagcgt gcccaagggg ggcctcgccc tgccccccct gctgctcgcc gatcagccgg
    10141 acctgcaggt ggtggccgtc accgaccagg gtcggatgct cgtcttcccg ctgacggagg
    10201 tgcccgaact gacccgcggc aagggaaaca agcttctcaa cattccgtcc gcggctttca
    10261 aatccggcga agagcggctg gttgcggtga cggtcattcg tcccgacgac gagctgctcg
    10321 tgctggcggg gcagcgtcat ctccgcatga agcacaggga tctcgagaac tacgttggtg
    10381 agagggcacg gcgcgggcgc aagcttccgc gcggatttca gaaagtagac tcgctggcag
    10441 ttgcgggacg gtgaccggcg aggtgggcgg cctggtgctg taccagatcc tgctgcgggg
    10501 ctgacagcgc tgtaatcagg accgagtcgg gacccaccgt tgccagctcc tcgagcaggg
    10561 ggtgctcgat gaccgtgcgc tccaccagct cgccgccgcg gtcgaggcag gcggagaaaa
    10621 cgctgctcgc ggccagggtg gccggcttgg cgagcgcgga caggagcgcg gcatcgccga
    10681 ccgcctgctc gtccaacagc acgcgatggc cgaccggcgc gtcgccgacg tgaatggcca
    10741 ctcgatagtc acccgcctcc tcgtcgagaa gctccgagag caccgccgct gcgctcagca
    10801 cctgccaggg acggtccgca tcgcccccgt cgttgaagtc cagcagcaat ccggtaccgg
    10861 gcagattttc cacatcgccg ccgtacaggc cggcgacctt gcccgcggcg atgcgcgcgt
    10921 agttcagctc ggcgacgcgt tgcgtgggct ccatgccgag ctggtcgtac aggttcacgg
    10981 cgacgaggaa gtgcctttcc cgcggcgtgt gaagctccac ctctatacgc gcgggctgcc
    11041 tcggcagggg cggttgaagc cggatgctga gcagggccag cgccaggaag ggcgcagcaa
    11101 tcaaggtgac agcggccacc gccacggcat ccccgaggtg gcttggaggc tcgctcaggg
    11161 cgacccgcac ggcgcccatg atctggtcgt tgaaggtcac cgggctctgg tagacgcgcg
    11221 cgttcgaaag ggagccgctc gaggcgagcg cctgctcgtt gaccgttacg atttcgaccc
    11281 cgctgacgcc gggaatgtcc gccgcctgat tggcgatcac gcccagcgtg atgcgatcgt
    11341 gggtcagcag cggctcgatg gccagctggg cgagccctgt ggacatgctc ctgccatagg
    11401 cgtcgtgacg gagctgatcc ggatcgttga gagcccagta ggacagcatc acggccacgg
    11461 aaaccagcga gcccaggcaa gcgccgacca gcgaaagcgg cggtggtttg acgagcccca
    11521 gccaagacgt tagcttcaaa ctacttccta ttccgcgcgg cgatgtgagc agcgagattg
    11581 tattggtgaa cgtgtccgga gaggaccagc ctggcctgat ggccatgttg acatccactc
    11641 tggcacgtta tcaagtgcgg gtgctcgacg tggggcaggc ggtgatccac gacgagctca
    11701 acctcggcat gttgctgcag atcatcagtg acggtaacgg cgtggaggcc gccgaggtgc
    11761 tgtccgccat cgaggcgaac gttctcgatc agggcatgac ggcgcgcttc actcccatct
    11821 cggcgggcga ctacgccgac tgggtaaaga gccagggcaa gccccgctat atcgtcaccc
    11881 tcctcacggc cggcgccacc ggcgagcagc tcgccgcggt gacctccatc gttcgtcacc
    11941 acggcctcaa catcgacgcc atacgacggc tgtcggggcg cttgcccttg acgtccgacg
    12001 ccggtggtca gcggatctgc atcgagatga gcctgcgcgg cgtccttgcc gatgccggcg
    12061 ccctgaaagc gggccttctc gaagccgccg agcgcctggc gttcgatttc agcgtgcagc
    12121 aggacaccgt gtaccggcgc aaccggcggc tggtggcgtt cgacatggac tctacgctca
    12181 tcgatgccga ggtcatcgac gagctggcgg ccgcgcacgg ggttggcgag caggtagctc
    12241 ggatcaccga gcgagccatg cgcggtgagc tgaagttcgc cgacagcttt cgagagcggg
    12301 ccatgctgct caagggtctt tccgaggacg ccctgcgtga ggtggcagac ggcgttgcgc
    12361 tgaacgacgg tgcccggcgc ctgatccgca cgctcaagca tttcggctac aagacggcca
    12421 tcatctcagg cggctttcag tacgttggtg agcggctgcg tgacgagctg ggcatcgatt
    12481 acgtttatgc caactcgctg gagattcgcg acgggctgat gtcgggcgag gtcgaagggg
    12541 agatcataga cgccggccgc aaggccgagc tcttgaagga gatcgcccgc cgtgagggga
    12601 tatccggcca gcagaccatc gccatcggcg acggcgcgaa tgacctgccc atgctcaatg
    12661 ccgccggtct tggggtggca taccacgcga aacccatggt caaggagacg gctagccacg
    12721 ccatctccac cttcggcctg gacagcgttc tgtacctgat gggcttcagc gaccgcgaca
    12781 tcgacgaagc gcttacgggc tgaagccgcc gccccggcga tatccggtca gcggggatca
    12841 gtcgtcggcg ctgaagtcgt aatccatact tctggagggc tcctgcaggt agtgcccggc
    12901 cacgtagttg gcaccggcct gccatagggt cgacaggatg ttggcgcttt ccaccatggg
    12961 gaccaccgtg agcttgccgg cgccctggag gccgtgaatc atctcggtca gcgcctcacg
    13021 cgcctccgag ctgctggcga tcttctcgat cagcgaaccg tcgaccttga cgtattccgc
    13081 cgggatttgt tcgagcagtt cgaaagagtt gccgaggcgc ccaaatcgcg acagcgaggc
    13141 tacgcagtgc atctgcttca actgctcgaa gaactcgcgg gtctggcgaa cgtaggtgtt
    13201 ggcgtcctgc tccgtgatct gcaggaccac cgcatcggta ggcagccgaa cggccttgaa
    13261 ggcgacgcca agccactcca ggaactccgg gtcgttgacg ctgttgctgg tcaggttgat
    13321 ggtcagccgg gtcaggttgc ccttggcacg gtgcgcagac agcgacttga tcgcctggag
    13381 aatgacccat cggtcgatcc gtccggcgag gttgttctcg atggccgtgc gcaggaaagc
    13441 gcccgggagc atctgtttgc cttcgtcgtc aagcatgcgc agcagcactt cgtagtgctc
    13501 gtcggagtcg ccgtgcaggc tgatcatcgg ctggaacagc agaacgaatc gctggtcgtc
    13561 gatggcctcg ttgatcaggc gcagcagcct tgctgattcg gactccggct cgtcgcctgc
    13621 tccctgggac ttcagctcga cgcagtttcc ttcgttctgt tccatggatg tgcgcaggac
    13681 atcgtaggcc tcgttcagac agtcctccaa ccgctcacgg acgccctgat cttcctccgt
    13741 ggcgatctcc atgccggcga gggtaatcgt gggccgtacg gtcttctccc tgacctcgag
    13801 cagcgatgcc tcgatggtcc cgcgaagctg atcggcctct gctcggacct cgtcctcgtt
    13861 gtcggcggtt accgcaaagg cgaactgatg atccgccagt cgatgcagcg cgtccgatgc
    13921 gatcagctcg ccgagacacc gctcgacatg acggcagacc tcgagcgctc cagccagacc
    13981 gtgagatcgc tgcacctcgg cgtaatcg
//
LOCUS       DBNHKV010000443         9739 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1126178, whole genome shotgun sequence.
ACCESSION   DBNHKV010000443 DBNHKV010000000
VERSION     DBNHKV010000443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9739)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9739)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9739
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1126178"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1381
                     /locus_tag="AC16PP_14480"
     CDS             56..1381
                     /locus_tag="AC16PP_14480"
                     /inference="COORDINATES: protein motif:HMM:NF028240.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HIR6482883.1"
                     /translation="MIDLSNSFPTARRKPLLSVVSMVLAMLVVPPVQSSEVDLLIEKL
                     VAKGILNEGDAKEIRAEVARERQQAAAAPVEIQDLGTLPVAHAPAEAKEHWSDKISFK
                     GDLRVRYQGEELDNAPSIGPFDIDERNRWRIRWRAGVEADVSDRWEVGFGLASGGSDA
                     RSSNQTLRNAFSTGDARLDYAFARFKAGDGVDVVAGKFKNPIWKPKDLLWDSDIRTDG
                     VAVPMEFGVNERVSAFVTPAYLVLTEDVIGGQDDAAMWLLQAGANFDLSDAVSLTVAP
                     TYYNFVNLKGSKGPITVDVPSNARGEDDRFVYDYDSIALGGKLDIDGTELIPRVSVFG
                     EWVKAFDPDEEDTGWLVGLALGDAKVSNFGDWQLKYNYRRLEADAWPEFLSDSDFFFG
                     ATNVKGSEFEFIWGLAKGVNVSVDYYTAAKFIGSDLEQDLLQLDLNVKW"
     gene            1505..2176
                     /locus_tag="AC16PP_14485"
     CDS             1505..2176
                     /locus_tag="AC16PP_14485"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6482884.1"
                     /translation="MGEKAQITSADGFTLGAYQATPAGQPKGAVVVIQEIFGVNQHIR
                     EVVDGYAADGYFAIAPQIFDRVERDIELGYEEADMGAGIEIAFQKLDHAKALEDVQAA
                     IEVASVQGKVGVVGYCFGGLLTWLAACELEGVSAASSYYGGGVAGEAQRQPRCPVIMH
                     FGELDAHIPMSDVEKVKAAQPDVPVFVYPADHGFNCDHRASYDAAAAATAKQRTLEFF
                     GRELA"
     gene            2445..3233
                     /locus_tag="AC16PP_14490"
     CDS             2445..3233
                     /locus_tag="AC16PP_14490"
                     /inference="COORDINATES: protein motif:HMM:NF023358.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 domain-containing protein"
                     /protein_id="HIR6482885.1"
                     /translation="MNTQRIHRSWGPVPLLLALLGLALCGNASAASLNDIFVVTDRLN
                     TQAKRSQAKIDALTEETRKLLSEYKTVLKEIEGLRVYNRQLEKQISSQEAEMAKLASS
                     IDQVTLIERQITPLMLRMIDGLEQFVELDLPFLQDERAGRIEALREMMDRADVAVSEK
                     FSQILRAYQIENEYGRTMEAYGDTINVAGVDLKADVLKVGRVTLVYQTPDGASTGRWD
                     PKAGQWVELGDEYTTPVRSGIRMARKQLSVDMLTMPVAAPEAAE"
     gene            3233..4690
                     /locus_tag="AC16PP_14495"
     CDS             3233..4690
                     /locus_tag="AC16PP_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6482886.1"
                     /translation="MNHVKIKIGAGILALGLNGAALAEEAAPDVVVNQADTLEELLRN
                     VEERRVTESREHTQREQVFVREKNQQARMLADAQAERRREEQRSERLETAFEENEIRI
                     GDLQEQLDRRLGSLRELFGVLQQVAGDTRGLFEGSIISAEFPNRGEWLGQLAAKMGTA
                     SELATIDEMETLWFELQREMIESGKVSKFPGTIIQLDGQRKDADIIRVGAFALISEGE
                     YIQFDVNDGSLTELARQPSGRYTGSAEDLQEAGAGDVVAFAVDPTRGSLLSLLIQAAT
                     VGERVGSPFGGIAKGECYLPFCDGQGGYVGAVIILGGLIGVLLAVERLVTLSMVGAKV
                     ASQKKSSTPNEDNPLGRVLKVYEENKDVDVETLELKLGEAILGETPKLTRNIPLILVI
                     SVVAPLLGLLGTVIGMILTFQAITLFGTGDPKTMAGGISTALMTTVLGLCVAIPTTLL
                     HSIVATRSRSVIHVLEEQSAGIIADHAEQSGRALG"
     gene            4703..5221
                     /locus_tag="AC16PP_14500"
     CDS             4703..5221
                     /locus_tag="AC16PP_14500"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR6482887.1"
                     /translation="MINDAYIAIISFMEQGGNVLWLIAALTFLMWTLIFERIWYFQSE
                     HKSVVRIATETWENRAERRSWSAHQIRDSIISDASEKITGSLPLIQTCVTLCPLLGLL
                     GTVTGMISVFDAMATQGGNARSMAAGVSMATIPTMSGMIASLSGLMGSTFVKRKVDFE
                     IELFQDHLTMDH"
     gene            5240..5647
                     /locus_tag="AC16PP_14505"
     CDS             5240..5647
                     /locus_tag="AC16PP_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR6482888.1"
                     /translation="MRREANRFSRTDEESDINLTPMLDVVFIMLIFFIVTATFIKQAG
                     IEVFRPEAMTAEQKPTVSVLIAIGPTGEIWIDKKRVDASAVRAHIERLHAENPKGGLV
                     IQADREAKSEKLMAVLNAARAAGLTEVAISTEA"
     gene            5671..6267
                     /locus_tag="AC16PP_14510"
     CDS             5671..6267
                     /locus_tag="AC16PP_14510"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR6482889.1"
                     /translation="MRYVFGVALGIVTTFALFLLMQALIKSDKSPFDEGVKGKIVDFV
                     RLQDDVDIQTKQRKPKPPPPPDEPPPDMPKPDFDSNVDSQGVDIGSVNINVDLNVGGT
                     GGFSSDGEYLPIVKVAPVYPRRAQTRGIEGYVLLEFVVTKTGAVRDPVIIESKPPGIF
                     DRAALQAALKFKYKPKVVNGEPIDVAGVRNRITFELQD"
     gene            6298..7635
                     /locus_tag="AC16PP_14515"
     CDS             6298..7635
                     /locus_tag="AC16PP_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6482890.1"
                     /translation="MKFCSHIVRLALSVTLCFAAIPFAGEFGLSGSQAFAEEGEGQQQ
                     KTRRVPSMSEATFKKLAEAQELIDAKDYQGAVTSLNEMLTSRRRSLNGNEVGQVHNML
                     GFVYFSMEDYPKAIEMYKVVVDQEEDIPEGLEVTTLYTLAQLSFVAERYRDALKYMEI
                     WLSKAQNPGPEPHIFMGQVYYQMQDFRRSIVQIEKGIGVAQERGTPVKENWWSLLNYL
                     YFEEENWPKVIEILEILVRDFPKREYWIRLAGIHGQEGNEREQIGSMEAAHVAGFLTR
                     ERDLTNYAGLLMQAEVPIRAAKVLDAGVEAGVIEKTATNLRSLGQAWQLAQETKKAIP
                     VFEEAGKLADDGAIYERLAQLYLDNDEFSKCVRAAGRAIDKGGLRKVQTTYVVRGMCE
                     YNQDRLPQARSSFVSCRNESRRAKDETNRRICQQWITYLDRETTRREQLEKAI"
     gene            7707..8351
                     /locus_tag="AC16PP_14520"
     CDS             7707..8351
                     /locus_tag="AC16PP_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase subunit alpha"
                     /protein_id="HIR6482891.1"
                     /translation="MFTGIVQGLCPVTGVDDKPGLRCIAIKLGKLAGGIQTGASVAIN
                     GTCLTVTSAAKGVVSFDVIRETLENTNLGDLQRGSLVNVERSFRAGDEIGGHILSGHV
                     IGKVRVAAVEEDENERNVRFDVRAEWIKYLPHKGFVALDGASLTIARVDATNDQITVC
                     LIPETIARTTLGSAEPGSWVNLEIDAQTQAVVDTVERVLSSPEWQRRLRGEPSS"
     gene            complement(8401..>9739)
                     /locus_tag="AC16PP_14525"
     CDS             complement(8401..>9739)
                     /locus_tag="AC16PP_14525"
                     /inference="COORDINATES: protein motif:HMM:NF019410.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HIR6482892.1"
                     /translation="TIETSQLLSMLSDVQGDRTVHSIDPASVPESLDLQAVLAARSAQ
                     VLGHVNASPARDDGDAMNFVAMLFDYILNDRNLAIPMKALIARLQIPIIRLAIIDKSF
                     FEKSNHPARQLLNELSSAGIGWSAASELKRDALYNFIESIVLRVLNDFADNPDLFETL
                     VEELREFLKKDLKRRLLMEKRVKEIETGKAKTKAAKETVQNLINEMASGQRVPPEIGK
                     FISETWSKALIFACVTAGTESDLWNHHVRVMADLLKIIQPLQSEDDIAARDAVAPDLI
                     DTIEAALVDLKIADRERNDLITMLTEQLRQLSADDRAHVDEPESAAPVLAFETMRNVT
                     LTAPTEAEEQDPDDPPPPQYVDEVNRLEEGAWLEFVDEHGDNVRVKLAGIIEPGARYV
                     FTNRRGIRVFKKSKMQLANDLQNQTVMVLDESQVFDRALQAVISKLRTEEAEE"
BASE COUNT         1927 a         2805 c         3133 g         1874 t
ORIGIN      
        1 gctgccagac ggcatccgat aacggcagat tgttaaccta cgatgaggtt gtcccatgat
       61 tgatttgtcc aactcgtttc caacggcgcg ccgcaagccc ctcctgagcg tggtctctat
      121 ggtcctcgcc atgctggtgg tcccgcctgt tcagtccagc gaggtggatc tgctcataga
      181 gaagctcgtg gccaagggaa ttctgaacga aggcgacgcg aaggagatca gggcagaggt
      241 tgcccgggaa aggcagcagg ccgcagccgc gccggttgag attcaggacc tcggaacgct
      301 gccggtcgcg catgcgcccg cagaggcaaa agagcactgg tccgacaaga tatcgttcaa
      361 gggcgacctg cgcgtgcgct atcagggcga agagctggat aacgccccca gcatcggccc
      421 gttcgacatc gacgaacgca accgctggcg gattcgctgg cgcgccggtg tcgaggccga
      481 tgtcagcgat cgctgggaag taggcttcgg gctggcctcg ggaggctcgg acgcgaggtc
      541 gagcaaccag acgctgcgca atgcgttctc cacgggcgac gcccggctgg actacgcgtt
      601 tgcccgcttc aaggccggcg acggcgtcga cgtggtggcg ggaaagttca agaatcccat
      661 ctggaagccc aaggatcttc tgtgggattc ggacattcgc accgacgggg ttgccgtgcc
      721 catggagttc ggcgtcaacg aaagggtctc tgcgttcgtt acccccgctt acctggtcct
      781 gaccgaggac gtgatcggag ggcaggacga cgcggccatg tggctgcttc aggcgggcgc
      841 gaatttcgat ctctcggacg cggtgtctct gacggtcgcg ccgacctatt acaacttcgt
      901 caatctcaag ggcagcaagg gtcccatcac cgtggacgtg ccgagcaacg cgcggggcga
      961 ggacgaccgc tttgtttatg actacgactc catcgccctc ggcggcaagc tggacatcga
     1021 cggcaccgag ctgatcccgc gcgtcagcgt cttcggcgag tgggtcaagg cattcgaccc
     1081 tgacgaggaa gacacgggct ggctggtcgg gcttgccttg ggcgatgcca aggtgagcaa
     1141 tttcggcgac tggcagctga agtacaacta ccgacgcctc gaggcggacg cgtggccgga
     1201 gttcctgagt gacagcgact tcttcttcgg ggcgaccaac gtcaagggca gcgagttcga
     1261 gttcatctgg gggctggcaa agggcgtcaa cgtgtcggtg gactactaca cggcggccaa
     1321 gttcatcggt tccgacctgg agcaggatct tctgcagctc gatctgaacg tgaagtggta
     1381 gcgcctctgg tgcttttcca gcggctgagg atgagctaag gttcgacccc gctcgacgcc
     1441 cgtcatgagg cgctgagcaa agcgcccgga ttgaggcggc accgacacca gggaggaacg
     1501 gacagtgggg gaaaaagctc agatcacgtc agcggacgga ttcactctcg gggcgtatca
     1561 agctacgccc gcagggcagc caaaaggcgc cgtcgtggtc attcaggaga ttttcggcgt
     1621 aaatcagcac atccgcgaag tggtagacgg ttatgccgcc gacggttact tcgccatcgc
     1681 gccgcagata ttcgatcgag tggaacgcga catcgagctg ggctacgagg aagctgacat
     1741 gggcgccggc atcgagatcg cgttccagaa gctcgatcat gccaaggcgc tcgaagatgt
     1801 gcaggctgcc atcgaggtgg cctccgtgca gggcaaggtg ggtgtagtcg gatactgctt
     1861 cggcggcctg ctcacctggc tggccgcctg cgaactcgag ggggtcagcg ccgcgtcttc
     1921 ttattacggt ggcggcgtgg cgggagaagc gcagcgtcag ccgcgctgtc cggtgatcat
     1981 gcatttcggc gagctggacg cgcacatccc gatgagcgac gtggagaagg tcaaggccgc
     2041 tcagccggac gtgcccgtct ttgtctatcc ggccgatcac gggttcaact gcgatcatcg
     2101 ggcaagctat gacgcggccg cggcagccac tgccaagcag cgcacgctgg aatttttcgg
     2161 gcgggagctg gcctgaccgg cccgtgccca tgccgttccg ggcgaaagtc ccgtcaagct
     2221 tgacttccgt gaggggtttg atgatccttc gatcattgaa gaggggcggg gcacatcggg
     2281 cgtgtcggag tgcgtgcacc ccgcgttttg cggatgtaca gcaggcgaac gtgcactttt
     2341 ttcgcaacgg ctcaaacgtg cacaaaggtg tggtgcgcgg cgttttcagc gccggcattt
     2401 cctgctgttg cgctcattaa gataaccatt ccgtaggaag tcgtatgaac acacaacgaa
     2461 tccacaggtc ttggggacca gttccattgt tgttggcttt gcttggcctg gcgttgtgtg
     2521 gcaacgcgtc ggcggcatcg ttgaacgaca ttttcgtggt gaccgatcgc ctcaacactc
     2581 aggcgaagcg atcccaggcc aaaatcgacg cgctgaccga ggagactcgc aagctgctca
     2641 gtgagtacaa aaccgtgctc aaggaaatcg aaggcctgag agtctacaac cgacagctcg
     2701 aaaagcagat ttccagccag gaggccgaga tggccaagct ggcaagctcc atcgaccagg
     2761 tgacgctcat cgagcggcag atcacgcctc tgatgctgcg catgatcgat gggctagagc
     2821 agttcgtgga gctcgatttg ccgtttctgc aggacgagcg tgcgggccgg atagaagctc
     2881 tgcgcgagat gatggatcgg gcagacgtgg ccgtatccga aaagttcagt cagatacttc
     2941 gcgcctatca gatcgagaac gagtacgggc gcaccatgga agcttatgga gacacgatca
     3001 acgtagcggg tgtggatctc aaggcggacg ttctcaaggt ggggcgggtg acgctcgtgt
     3061 atcagacgcc cgatggggcc tccaccggcc ggtgggaccc aaaggccgga cagtgggtcg
     3121 agttgggaga cgagtacacc accccagtac gaagtggtat ccggatggcg cgcaagcagc
     3181 tctccgttga catgttgacg atgcccgtcg cggctccgga ggctgcagag tgatgaatca
     3241 cgtaaagatc aagattggcg ctggaattct ggcgctcggg ctcaatggcg ctgctcttgc
     3301 cgaagaggcc gcgccggacg tggtcgtaaa ccaggccgat actctcgaag agctcctgag
     3361 gaacgtcgag gagcgcaggg tcacggaatc ccgtgagcat actcagcgcg agcaggtgtt
     3421 cgttcgcgaa aagaaccagc aggcgcgcat gctcgccgat gctcaggcgg aacgccgacg
     3481 tgaggagcag cgttcggaaa ggctggaaac cgccttcgaa gagaacgaga tacgtatcgg
     3541 cgacctccag gaacagctcg accggcgctt aggctccctg cgcgagcttt ttggcgttct
     3601 ccagcaggta gccggcgata cccgcgggtt gtttgaaggc tcgatcatct ccgccgagtt
     3661 ccccaaccgt ggagaatggc tcggccagct cgcggccaaa atgggcaccg cgagcgagct
     3721 ggcgaccatc gacgagatgg aaaccctctg gttcgagctc cagcgtgaaa tgatcgagtc
     3781 gggcaaggtc tccaagtttc ccggaaccat catccagctg gacgggcagc gtaaggacgc
     3841 cgacatcatc cgcgtgggtg cctttgctct gatctctgaa ggcgagtaca tccaattcga
     3901 cgtcaacgac ggtagtctga ccgagctggc tcggcagccg tccggccgct acacgggttc
     3961 agccgaagat ctgcaggagg ccggtgccgg cgacgtcgtg gccttcgcgg tggacccgac
     4021 tcgtggctcc ctgctgtctc tgctgattca ggccgcgacc gtgggcgagc gggtgggttc
     4081 tccgtttggc ggcatcgcca agggcgagtg ctacctgcca ttctgcgacg gtcagggtgg
     4141 ctacgtaggc gccgtgatca ttctgggcgg gctgatcggc gtgctgctcg ccgtggagcg
     4201 gctggtcacg ctctcaatgg tgggcgcgaa ggtcgcctcg cagaagaaat cgagcacgcc
     4261 gaacgaggac aatcctctcg gccgggtgct gaaggtctat gaagagaaca aggacgtcga
     4321 cgtggaaacc ctcgagctca aactcgggga agccattctc ggcgagacgc cgaaactgac
     4381 gagaaacatc cccctgatcc tggtgatctc ggtggtcgcg ccgctccttg gcctgctcgg
     4441 cacggtgatc ggcatgatcc tgacattcca ggccatcacc ctgttcggca ccggcgatcc
     4501 caagacaatg gctggcggca tctccaccgc gctcatgacg acggtgctcg gtctgtgtgt
     4561 cgccataccg acgacgctgc tgcactccat cgtggcgacc cgcagccgat cggtgatcca
     4621 cgtcctggaa gagcagagcg ccggcatcat tgccgatcac gcagagcagt ccggaagggc
     4681 gttggggtaa cggtaagctc ccatgatcaa cgacgcctac attgcgatca tcagcttcat
     4741 ggagcagggt gggaatgtgc tgtggctcat cgccgccctc acgttcctga tgtggacgct
     4801 gatcttcgag cggatctggt actttcagtc cgagcacaag agcgtagtgc gaatcgccac
     4861 ggaaacctgg gagaaccgtg ccgagcgccg gtcctggagc gcccatcaga tcagggactc
     4921 catcatctcc gacgcttccg agaagatcac gggcagcctc ccgctgatcc agacctgcgt
     4981 gacgctctgc ccgcttctcg gcctgctagg tacggtgacc ggaatgatct ctgtattcga
     5041 tgccatggcc actcagggcg gtaatgcccg ttccatggct gccggcgtgt ccatggccac
     5101 gataccgacg atgtccggca tgatcgcttc gttgtcgggg ctgatgggca gcaccttcgt
     5161 gaaacgaaaa gtcgatttcg agatcgaact gttccaggac catctgacga tggatcactg
     5221 atccacgacc ggaggaagta tgcgtcgaga agcaaaccgg ttttcccgta ctgacgaaga
     5281 gtcagacatc aacctcacgc ccatgctgga cgtggtgttc atcatgctca tcttcttcat
     5341 cgtcacggcc actttcatca agcaggcggg tatcgaggtt tttcggcccg aggcgatgac
     5401 ggccgagcag aaacccacgg tcagcgtgct gatcgccatt ggaccgaccg gggaaatctg
     5461 gatcgacaaa aagcgtgtgg acgccagcgc cgttcgcgcc cacatcgagc ggcttcacgc
     5521 cgagaacccg aagggcgggc tcgtcattca ggcggatcgt gaagccaaat ctgagaagct
     5581 catggccgtg ctgaacgcgg cccgagcggc cggtctcact gaggtcgcga tctccacgga
     5641 ggcctgaggc caggaggcat cactatggtc atgcggtacg ttttcggagt tgcgctgggt
     5701 atcgtcacga ccttcgctct ctttctcctg atgcaggcgc tgatcaagag tgacaagagc
     5761 ccgttcgatg aaggcgtcaa gggaaagatc gtcgacttcg tccgcctgca ggatgacgtc
     5821 gacattcaga ccaagcagcg caagcccaag ccgccgccgc cgccggacga gccgcccccg
     5881 gacatgccca agcccgactt cgactccaat gtggattccc agggcgtcga cattggttct
     5941 gtgaatatca acgtggatct gaatgtcggc ggtacgggcg gattttcgtc agacggtgaa
     6001 tatctgccca tcgtcaaggt ggcccccgta taccccaggc gggcgcagac tcgaggcatc
     6061 gaggggtacg tgttgctgga gttcgtggtg accaaaaccg gggccgtccg ggatcccgtg
     6121 attatcgaat ccaagccgcc tggaatcttc gatcgggctg cgctccaggc cgctttgaaa
     6181 ttcaagtaca agcccaaagt agtcaatggc gagcccatcg acgttgctgg tgtgcgtaac
     6241 cgtattacgt tcgaacttca ggattgacgg ggcttggggc taagccggga ggtagatttg
     6301 aagttttgtt ctcacatcgt tcgcctggcg ctgtccgtga cgttgtgctt cgcggcaata
     6361 ccgtttgcgg gtgaattcgg cctgagcggt tcccaggcct tcgccgagga aggcgagggc
     6421 cagcaacaga agacgcgtcg agtgccttcc atgagcgagg cgactttcaa aaagcttgca
     6481 gaggcgcagg agctgattga cgccaaagat tatcagggcg cggtcacgtc cctgaacgaa
     6541 atgttgacga gtcgccgtcg gtcgcttaac ggcaacgagg tcgggcaggt gcacaacatg
     6601 ctcggtttcg tctacttctc catggaggat taccccaagg cgattgagat gtacaaggtc
     6661 gtcgtcgatc aagaggaaga tattcccgag ggcctcgagg ttacgacgct atacaccctg
     6721 gcgcagctga gcttcgtcgc tgagcgctat cgggacgcgc tcaagtacat ggaaatctgg
     6781 ctctccaaag ctcagaaccc ggggcccgag ccccacattt tcatgggcca ggtctattac
     6841 cagatgcagg actttcgccg ctcgatcgtc cagatagaga aggggatagg cgtcgcccag
     6901 gaaaggggaa cgcccgtcaa ggagaactgg tggtcgctgc tcaactatct gtatttcgag
     6961 gaggagaact ggcccaaagt gatcgagata ctcgagattc tcgttcgcga cttccctaag
     7021 cgcgagtact ggatccgcct tgccggcatc catggccagg agggcaacga gcgggagcag
     7081 atcggatcca tggaggccgc gcacgtggcg ggcttcctca cccgcgagcg ggatctgacc
     7141 aattacgccg ggctgctgat gcaggccgag gtgcctatac gagcggccaa ggtcctcgac
     7201 gccggcgtcg aggccggcgt gatcgagaag acggccacga atcttcggag ccttgggcag
     7261 gcatggcagc tggcccagga aaccaagaag gcgattcccg ttttcgagga ggcgggcaaa
     7321 ctcgcggacg atggggccat ctacgaacgg ttggcccagc tctatctcga caacgacgag
     7381 ttcagcaagt gcgttcgtgc ggccggaagg gccatcgata agggtgggct gcgcaaagtt
     7441 cagaccacct acgtggtgcg aggcatgtgt gagtacaatc aggaccggct tccccaggca
     7501 aggtcatcgt tcgtctcctg tcgtaacgag tcgcggcgcg ccaaagacga gaccaaccgg
     7561 cgaatctgcc agcaatggat cacctatctg gatcgcgaaa ccacgcggcg cgagcagttg
     7621 gaaaaggcca tctgaagccg aacctgctcc cgactgacgt cccacgtccg ggccggcatt
     7681 gccggccctc tgcgtaaagg gctccaatgt tcaccggtat cgtccaaggc ctctgcccgg
     7741 tcactggcgt agacgacaaa ccgggccttc gatgcatcgc catcaagctg ggcaagctgg
     7801 cgggtggtat ccagaccggt gcgtccgtcg ccatcaacgg aacctgtctg accgtgacgt
     7861 cggctgcaaa gggcgtggtg tctttcgacg tcattcgcga gacgcttgag aacacgaacc
     7921 tcggagatct gcaacgcggc tccttggtca acgtcgaacg gtcattccgt gcgggggacg
     7981 agataggggg tcacatcctg tcgggtcacg tcatcggcaa ggttcgcgtc gcggccgtcg
     8041 aagaggatga gaacgagcgg aacgtgcgct tcgacgtgcg tgctgaatgg atcaagtatc
     8101 tgccccacaa aggcttcgtg gcgctggacg gagcgagcct gacgattgcc agggtcgatg
     8161 caaccaatga ccagatcacg gtatgtctga tccccgagac cattgcccgc acgacgctgg
     8221 gaagtgctga gccgggatcg tgggtcaatc tggagatcga cgctcagacc caggccgtgg
     8281 tggacacggt tgagagggtg ctgagctcgc cggagtggca aaggcgtctg cggggcgaac
     8341 cgtcgtcctg accgtttctc tcagcccttc gaggccaatc ggcatcgggg tgcgcccgat
     8401 ctattcttcg gcttcctcgg ttctcagctt ggaaatgacc gcctgcaggg cgcggtcgaa
     8461 cacctgggac tcgtcgagaa ccatcacggt ctggttctgc aggtcgttgg caagctgcat
     8521 cttgctcttc ttgaagaccc gtatcccccg gcggttggtg aacacgtagc gcgctcccgg
     8581 ctcgatgatg ccggctagct tgacccgaac gttgtcgccg tgttcgtcga cgaactcgag
     8641 ccaggctcct tcttccagac gattgacctc gtccacgtac tgcgggggcg gcggatcatc
     8701 gggatcctgc tcctctgctt cggtgggagc ggtcagcgtc acgttgcgca tggtctcgaa
     8761 agcgagaacg ggcgccgcgc tttccggctc gtcgacatgg gcccgatcgt ccgccgacag
     8821 ctggcgcaat tgctcggtga gcatggtgat caggtcgttg cgttcgcgat cggcgatctt
     8881 cagatctacc agagccgcct cgatggtgtc gatgagatca ggggccaccg cgtctctggc
     8941 cgcaatgtca tcttcgctct gcaagggctg gatgatcttg agcaggtcgg ccatcacgcg
     9001 cacgtggtgg ttccaaaggt ctgattcggt acctgccgtc acgcaggcga agatcagcgc
     9061 cttgctccag gtctcgctga tgaacttgcc gatctcaggg ggcacgcgct gcccgctggc
     9121 catctcgttg atcaggttct gaacggtctc tttggcggct ttcgtcttgg ccttgccggt
     9181 ctcgatctcc ttgacgcgct tctccatgag caaacgccgt ttgagatcct tcttgaggaa
     9241 ctcccgaagt tcctcgacca gagtttcaaa cagatccggg ttgtcggcaa agtcgttcag
     9301 gacccgcagc acgattgatt cgatgaagtt gtacaacgcg tcccgcttga gctcggaggc
     9361 cgcgctccag ccgatgcccg cgctggagag ctcgttgagg agctggcggg caggatgatt
     9421 ggacttctcg aagaacgact tgtcgatgat cgccaggcgt atgatcggaa tctgcagccg
     9481 ggcgatgagg gccttcatcg gaatggcgag gttccgatcg ttgaggatgt agtcgaacag
     9541 catggccacg aagttcatcg cgtcgccgtc gtcccgagca gggcttgcgt ttacgtgacc
     9601 gagcacctgc gccgacctgg ccgcgaggac ggcctgcaga tccagcgatt ccggcacgct
     9661 ggcgggatca atggaatgaa ccgtgcggtc tccctggacg tcggagagca tcgagaggag
     9721 ttgggacgtc tcgatggtc
//
LOCUS       DBNHKV010000444         7466 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_559136, whole genome shotgun sequence.
ACCESSION   DBNHKV010000444 DBNHKV010000000
VERSION     DBNHKV010000444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7466)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_559136"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="AC16PP_14530"
     CDS             complement(<1..478)
                     /locus_tag="AC16PP_14530"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6482893.1"
                     /translation="MGRLDDKVAVITGAGRGIGREIALMMAGEGAKIVVNDLGGNMDG
                     TGTGMIADDVVAEIRAAGGEAVSNTASVAEVAGGESLLQSALGAFGAMDILVNNAGIL
                     RDRTIFKMEEADWDAVQAVHLKGHYCCTRPFARYIRENNRTGCRVLNFSSVSGLYGN"
     gene            complement(491..1723)
                     /locus_tag="AC16PP_14535"
     CDS             complement(491..1723)
                     /locus_tag="AC16PP_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6482894.1"
                     /translation="MDLGISERVKPLIDDITQYIHDYVVPNERAYAEQVDAGGRWTET
                     EAMEEMKEKAKERGLWNFFLPDSERGSGLNNLDYAHVAEVLGRYPLSSEAMNCAAPDT
                     GNMEVIERYGSEQQKEEWLRPLLEGKIRSAFAMTEPQVASSDAANISLEARLEGDEWV
                     LNGEKWWTSGIGDPRCKIMIVMCVTEPDAPRHSRQSQILVPVETDGVEVLKMMHVFGY
                     DDAPHGHGHVRFTDVRVPKDNIILGSGRGFEIAQGRLGPGRIHHCMRSIGVAERALEL
                     MCRRALSKEAFGKPIADLGGNYDKIADARINIEMARLLTLKAAYMMDTVGNKIARSEI
                     AQIKVAVPNIALKVIDDAIQIHGGAGVSQVFPLAKMWAGQRTLRLADGPDEVHRRAVA
                     RLELGKYPDKDPIRTDVW"
     gene            1848..2552
                     /locus_tag="AC16PP_14540"
     CDS             1848..2552
                     /locus_tag="AC16PP_14540"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6482895.1"
                     /translation="MTAPGDATVRLLNDLFGGGVKNAVVLMRHSAREFAPGRHDLDNP
                     LTEEGRDLARRLGERLPEDLTVRAYASPPERCMETAELVLAGYASTGGRVTRHRPVEA
                     LGVFYALDQMKMWQVMQSAGGMVPFLEAWFSGDVPQDAVMPPRLAAALVVRLMTDKLS
                     AMDGARRLDVCVSHDMTLHLVRDRLLGQSVRDVDVDFLDGLVAFEREGARWLASHHGE
                     PVKLDAQLLSLGGLWD"
     gene            complement(2629..3498)
                     /locus_tag="AC16PP_14545"
     CDS             complement(2629..3498)
                     /locus_tag="AC16PP_14545"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR6482896.1"
                     /translation="MAYPRPELLEEPAALAARLDDADLRIVDATVFLVPAERGYRAES
                     GRERYEQGHIPGAAFMDLIDAFSDTTTGLGFSLPDPGVIADALGRLGISDDSEVVVYS
                     TGHLMWATRAWWLLRYVGHQRASVLNGGFDAWRDLGLPFETGTARRAAVTYRPRPDAS
                     LFTDRDGMCRAMDEPGVCTVNALSPEVYAGTGTTNYGRPGHIPGSVNVPYEELLAGGR
                     FKPAGALAEALESTGLLDADRVVTYCGGGIAATLDGFACLLLGHDNVAVYDGSMSEWA
                     RDPSLPLNVGEAP"
     gene            complement(3575..4837)
                     /locus_tag="AC16PP_14550"
     CDS             complement(3575..4837)
                     /locus_tag="AC16PP_14550"
                     /inference="COORDINATES: protein motif:HMM:NF028178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR6482897.1"
                     /translation="MSKREPGLAAFAALMLGFTGTNLAGCSPSVTEVDLIMPELPVEQ
                     IIARKIADLVDEDSGLKINLIPLPDDSLSVLDALEQGYGDIAFAPNTNEHRDTITTIM
                     PIYPSVLHVGSIADRADGSLEELLDGARVFAGPPGSIPRQIGERIVSGLQLEKGLSFT
                     SDQDAAPDVIILFAPIDRRRVMSNPSLESLKLFSFGDPGDTRPGSIVDRTVLLNPTLR
                     PYVIPAGTYGSLTPDPVVTFAVDTLLVARESLDETLVFDLFEEFLRLRPALFSKRPEL
                     FQPIDDDIARAHWTFSLHPGALDYLQRDEPSLIERYSGVAEVLVTLLVGMVSGAFAIL
                     KIYQVRRKNRIDAFYTQVIRTRDSVAEGAGSEERAAALAQLRSLRERAYEMLVEEQLA
                     ADTSFLIFVLLTNDAIAQIDSPENGPSV"
     gene            complement(4830..5690)
                     /locus_tag="AC16PP_14555"
     CDS             complement(4830..5690)
                     /locus_tag="AC16PP_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482898.1"
                     /translation="MIDAAVRRFDAFFLTEAQETFEEDRNRLRLRLDTDYIEHHGWEF
                     SPRVKVNFHLPGLGERVRLVVNDAGGDEEDEGVELEDDENDVALRWVARKGKKTGISV
                     DGGVSFRGGEVDPFVRVNTSYKYRISENWRGQATNRLYYLARNGWRDDLRIYFNRPIG
                     ERFLFRSRSRLEYFEDKDSNPQLEQKFSLFQNISDRSAIAYEAIWRQDTAEDSIYDDE
                     EILIGPRDKYQQVKLRIRYRRNFWRPWMFVEFWPVVGWVEERDWDTVLGARMRLEINF
                     GWHAGSKLDE"
     gene            complement(5882..6319)
                     /locus_tag="AC16PP_14560"
     CDS             complement(5882..6319)
                     /locus_tag="AC16PP_14560"
                     /inference="COORDINATES: protein motif:HMM:NF017913.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HIR6482899.1"
                     /translation="MAFHMMQLIANIERWVVLGLLVLMLLVVVASSIELAIIIVQAAL
                     ENHFSEEPAYLLLDINELLGIFGFFLLVLIGLELLASVKMYLEDDIVHVELVMLIALI
                     AVSRKIIVIDYAKVDGVTVLGIAALVLVLSVGYYLMKRSHEPS"
     gene            6545..7204
                     /locus_tag="AC16PP_14565"
     CDS             6545..7204
                     /locus_tag="AC16PP_14565"
                     /inference="COORDINATES: protein motif:HMM:NF017534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6482900.1"
                     /translation="MQIRFWRDRRPWRPVSMHQSAPNPCLERHWGGLRLEHGSTVFVP
                     MCGKSDDLRWLLGRGHQGLGIELADLAIEAYFEEAGEPYEREDSDRLVRYRGPRTDIL
                     CGDFFALTAAELSAARAVLDRGALIALPPKMRARYVDHLLRVIPDRTDILLVTLEYDQ
                     NLVAGPPFSVLPEEVVSHFGPRCSIVPLESSRTDFLPPKFAAQGVDCAAETAYHIVKQ
                     E"
BASE COUNT         1268 a         2343 c         2430 g         1425 t
ORIGIN      
        1 agttgccgta gagaccggac acgctggaaa aattgaggac cctgcagccg gtgcggttgt
       61 tttcccggat gtagcgcgcg aaggggcggg tgcagcagta gtggcctttc aggtgcacgg
      121 cttgaaccgc gtcccagtcc gcctcctcca tcttgaagat ggtccggtcc ctcaatatcc
      181 ccgcgttgtt gaccaggatg tccatggcgc caaacgcgcc cagagccgac tgcagaaggc
      241 tctcgccgcc cgccacctcg gcgacggatg cggtgttcga gacggcctcg ccgccggccg
      301 cgcgaatctc ggccaccacg tcgtcggcga tcattccggt tccggtcccg tccatgttgc
      361 cgccgagatc gttgacgacg attttcgcac cttcaccggc catcatcagc gcaatctcgc
      421 ggccgatccc gcgacccgcc ccggtgatga cggccacctt gtcatccagt cggcccatga
      481 cgatcgcctc ctaccaaacg tccgttcgga tcggatcttt gtccgggtat ttgcccagct
      541 ccagcctggc caccgcgcgc cggtgcactt cgtcggggcc gtcggcaagg cgtagagttc
      601 tctgtcctgc ccacatcttg gccaacggaa atacctggga tacgccggcg ccaccatgga
      661 tctgaatggc gtcgtcgatg actttcagcg cgatgttggg taccgccacc ttgatctggg
      721 cgatctcgct cctggcgatc ttgttgccca cggtatccat catgtaggca gccttcagcg
      781 tcagcagcct ggccatctcg atgttgatgc gcgcatcggc aatcttgtcg tagttgccgc
      841 ctaggtcggc gatgggcttg ccgaacgctt ctttggagag cgcgcggcgg cacatcagtt
      901 ccaaagcccg ctcggcgacg ccgatggaac gcatgcagtg gtggatgcgc cccggaccca
      961 gtcggccctg ggcgatctcg aaaccgcgac cggacccgag aatgatgttg tcctttggta
     1021 ctcggacgtc ggtgaatcgg acgtgcccgt gtccgtgggg cgcgtcgtcg tagccgaaga
     1081 cgtgcatcat cttcaggacc tcgacgccgt cggtttccac cggcaccagg atttgcgact
     1141 gccgcgagtg tcgtggcgcg tccggctcgg taacgcacat cacgatcatg atcttgcagc
     1201 gggggtcgcc gatccccgag gtccaccact tctcgccgtt gagcacccac tcgtcccctt
     1261 cgagcctcgc ttccagggat atgttggcgg catccgagga agcgacctgc ggctcggtca
     1321 tcgcgaacgc cgagcggatc tttccttcca gcagcggccg taaccattcc tctttctgct
     1381 gctcggagcc gtaccgctcg atcacttcca tgttgccggt gtcgggcgcc gcgcagttca
     1441 tggcctccga ggagagcggg taccgcccca gcacctcggc gacgtgcgcg tagtcgaggt
     1501 tgttgagtcc ggaaccgcgt tcgctgtccg gaagaaaaaa gttccacagg ccgcgctcct
     1561 tggccttttc cttcatctcc tccatggcct cggtttcggt ccagcgaccg cccgcatcga
     1621 cctgctcggc gtaggcgcgc tcgttgggta ccacgtaatc gtgaatgtac tgagtaatgt
     1681 cgtcgatgag cggcttgacg cgctcagaga tgcctaggtc cattgcttcc ccctgggtgc
     1741 ccggtgcccg atgttcgagc gggacagtga atatctctat taaaaggcgt gagaattcga
     1801 tactaataca gaaggttacg tggtgctatt tgacaggggg gagtccattg accgcgcccg
     1861 gcgatgccac cgttcggctc ctgaacgacc tgtttggggg cggtgtgaaa aacgccgtcg
     1921 tgctgatgcg ccactcggct cgggagttcg cgcccgggcg tcacgatctg gacaacccgc
     1981 tcaccgagga aggacgtgat ctggcccgac ggctcgggga gcggttgccc gaggacctga
     2041 cggtgcgcgc gtatgccagc ccgccggagc gatgcatgga aacagccgag ctcgtcctgg
     2101 ccggttacgc gtccaccggc gggcgggtga ctcggcatcg cccggtcgag gcgcttggcg
     2161 tcttctatgc cctggaccag atgaaaatgt ggcaggtcat gcaatccgcc ggaggaatgg
     2221 tgcccttcct ggaggcttgg ttttccggtg acgttcccca ggatgcggtg atgccgcccc
     2281 ggcttgcggc agcgctggtg gttcgtctga tgaccgacaa gctttcggcc atggacgggg
     2341 cgcgacggct agacgtctgc gtgtcccacg acatgaccct tcacctcgtc cgcgaccggt
     2401 tgctgggcca gagcgtacgc gacgtcgacg tggactttct ggacgggctg gtcgccttcg
     2461 aaagggaggg cgcgcgctgg ctggcgagcc atcatggcga gcccgtgaag ctcgatgcgc
     2521 agctgctctc tctagggggc ctgtgggact gaggagagct ggacgcacgc ggcgcccggc
     2581 ccatggcgtg ccctaagtgt cctatgtgcc ctatgtgccc tatgtgtcct aaggggcctc
     2641 gcctacgttg aggggcaatg aggggtcccg cgcccactcg gacatggatc cgtcgtagac
     2701 ggccacgttg tcgtgcccga ggagcaggca ggcgaagccg tcaagggtgg cggcgatgcc
     2761 cccgccgcag taggtcacga cccggtcggc atcgagcagc cccgtggatt cgagcgcctc
     2821 ggcaagcgcg ccggccggct tgaagcgtcc gcccgccagc agttcttcgt agggcacgtt
     2881 cacgctgccc ggaatgtggc ctggccggcc gtagttcgtg gttccggtgc ccgcgtatac
     2941 ctccggtgag agcgcgttga ccgtgcagac acccggctcg tccatcgctc ggcacatccc
     3001 gtcccgatcg gtgaacaggc tggcgtctgg tcgggggcgg taggtcaccg ctgctcgcct
     3061 cgcggtgccg gtctcgaacg gaagccccag atcgcgccac gcgtcgaacc cgccgttgag
     3121 gacggacgct cgctgatggc cgacgtagcg cagcagccac catgcacggg tagcccacat
     3181 gaggtggccc gtcgagtaga cgaccacctc gctgtcgtcg gagatgccca ggcggccgag
     3241 cgcgtcggcg atgacccccg gatccggcag gctgaagccg aggcccgtgg tcgtatcgga
     3301 gaacgcgtcg atgaggtcca tgaacgcggc gccgggtatg tgcccctgct cgtagcgttc
     3361 gcgcccgctc tccgctcggt agccgcgctc ggcaggaacg aggaaaacgg tggcatcgac
     3421 gatccgcaga tcggcatcgt ccagccgcgc ggcgagtgcc gcgggttcct ctagcagctc
     3481 aggacgtggg tatgccattt gattctcctg ctggtttgtc ggtttggtgc tggacggcct
     3541 catcccggct cgtcctctcc gcgggcgtgc cgcgctagac ggatggcccg ttctccgggg
     3601 agtcgatctg ggcgatggca tcgttggtca gcagcacgaa gatcaggaag ctggtgtccg
     3661 cggccagctg ctcctcgacg agcatctcgt atgcccgttc ccggagcgat cgcagctggg
     3721 cgagcgccgc tgcccgctct tcgctgcccg cgccctctgc cacggagtcg cgggttcgga
     3781 tcacctgcgt gtagaaggcg tcgatccggt tctttctgcg gacctgatag atcttgagga
     3841 tggcgaacgc tccgctcacc atgccgacca gcagcgtgac cagcacctcg gcgacgccgg
     3901 agtaacgctc gatgagcgaa ggctcgtcgc gctgcaggta gtcgagcgcg ccgggatgca
     3961 ggctgaaggt ccagtgggcg cgggcaatgt cgtcgtctat gggctgaaag agctccgggc
     4021 gcttgctgaa gagcgcggga cgcaggcgga gaaactcctc gaacagatcg aacaccagcg
     4081 tctcgtcgag gctttccctg gcaaccagca gggtatcgac ggcaaaggtc accacagggt
     4141 ccggcgtgag cgatccgtag gttcctgccg ggatgacata gggccgcagc gtcgggttca
     4201 gcagcaccgt tcgatcgacg atggagcctg gcctggtgtc gccggggtcc ccgaatgaga
     4261 agagcttgag gctttcaagg ctcggattgc tcatgacccg cctccggtcg atgggcgcaa
     4321 acaggatgat aacgtcgggg gccgcgtcct gatcggacgt gaacgataag cccttttcga
     4381 gctgcaaccc gctcacgatc cgctcgccga tctggcgggg tatcgaaccc ggcggcccgg
     4441 caaatacccg agccccgtcg agcagttcct ccaggctgcc gtcagcgcga tcggcgatcg
     4501 agccgacgtg gagcacgctc ggatagatgg gcatgatcgt ggtgatggtg tcgcgatgct
     4561 cgttggtatt cggcgcgaac gcgatgtcgc cgtaaccctg ctccagcgcg tcgaggaccg
     4621 agagggagtc gtcaggtaac gggatcagat tgatcttcag gccggagtcc tcgtccacca
     4681 ggtccgcgat cttgcgcgcg atgatctgct cgacgggcag ctcgggcatg atcagatcga
     4741 cctcggtcac cgaaggggag cagccggcga ggttggttcc ggtgaagccg agcatcagcg
     4801 ccgcaaaggc agcgagcccg ggctcgcgtt tactcatcga gcttagagcc ggcatgccag
     4861 ccgaagttga tctcgaggcg catccgggca ccgagcacgg tatcccagtc gcgctcctcc
     4921 acccagccga ccaccggcca gaactcgaca aacatccagg gccgccagaa attccggcgg
     4981 taacgaatgc gcagcttcac ttgctggtat ttgtctcttg gcccaatcag gatctcctcg
     5041 tcgtcgtaga tcgagtcctc agccgtgtcc tggcgccata tcgcctcgta ggcgatggct
     5101 gaccggtcgc tgatgttctg aaagagtgaa aacttctgct ccagctgggg gtttgagtcc
     5161 ttgtcctcga agtattccag gcgggaccgc gagcggaaca ggaagcgctc gccgatgggc
     5221 ctgttgaagt agatgcgcag gtcgtcccgc cagccgttgc gggccaggta gtaaaggcga
     5281 ttggttgcct ggccgcgcca attctcggag atccggtatt tgtagctcgt gttgacgcgg
     5341 acgaaggggt cgacctcgcc gcctcggaag cttacccccc cgtcgacgct gatgcccgtc
     5401 ttcttgccct tgcgggccac ccatcgcagc gcgacgtcgt tctcgtcgtc ctccagctcc
     5461 accccctcgt cctcctcgtc accgccagcg tcgttgacga ccaggcggac cctttcaccg
     5521 agcccgggaa gatgaaagtt gaccttgacc cggggcgaaa actcccagcc gtgatgctcg
     5581 atgtagtctg tgtccagacg cagccgaagg cgatttcgat cctcctcgaa cgtctcctgg
     5641 gcctcggtca ggaaaaacgc gtcgaaccgg cgcacggcgg cgtcgatcat ccctgcggtc
     5701 gatgtgtgaa gatcgtctac ttttgtcggt cctggctcga cctgatccgc gtcgggcggg
     5761 ggggtgtcgt tcgccgtccg accgggttgg gcagatgagt caacgggctc ggccgatgcg
     5821 ctcagcggca gggccatgag cccggcgagg aggattgtcg agactgaagt ccaggggaat
     5881 ctcaagacgg ttcgtggctg cgtttcatga ggtagtagcc cacggaaagc acgagcacca
     5941 gcgcggcgat acccagcacg gtaacgccgt cgacctttgc gtagtcgatc acgatgatct
     6001 tgcgcgatac ggcgatcaga gcaatcagca tcacgagctc cacgtggacg atgtcgtctt
     6061 cgaggtacat cttcacgctg gccagcagct cgagcccgat caggaccaga aggaagaaac
     6121 cgaagatgcc gagcagctcg ttgatgtcga gaaggagata ggcgggctct tccgaaaagt
     6181 ggttctcaag ggctgcctgg acgatgatga ttgccagttc gatcgaactc gccaccacca
     6241 cgagcagcat gagcacgagt agaccgagaa ccacccagcg ttcgatgttg gcaatgagct
     6301 gcatcatatg aaaagccatc ccgcgccgct tagccagggc gacgattaga ttagagaggg
     6361 cccggggcga tgtccaatcg gcgtccgtcg gcgttggcgg gcagcggccg gagaaaccac
     6421 cgccgtggat ccgctgcgcg ggccccaaag tggttgccgc aggcgacggc attggccaga
     6481 atgcggtcaa cagggaggta aacatcgccg ggggcgcagc gcctggcggt gtagaagaat
     6541 aacgatgcag atccgcttct ggcgagaccg ccggccctgg cggccggtca gcatgcacca
     6601 gagcgcccct aacccgtgct tggaaaggca ttggggcggt ctcaggctgg agcatggctc
     6661 caccgtgttc gtgcccatgt gcggtaagag cgacgacctg cgatggctgc ttggccgggg
     6721 ccatcagggt ctcggcatcg agctggcgga cctcgctatc gaagcttact tcgaagaggc
     6781 tggggagcct tacgagcggg aggattcgga ccggctcgtc cgctatcgtg gtcctcgaac
     6841 ggacatcctc tgtggcgact tcttcgcgtt gacggccgcc gagctctccg ctgcgcgcgc
     6901 cgtcctggat cgcggcgcgc tgatcgcgct gccgccgaag atgcgagccc gctacgtcga
     6961 tcacctgctc agggtcattc ccgaccgcac cgacatcctg ctcgtcaccc tcgagtacga
     7021 tcagaatctg gtggcgggcc cgccgttctc ggtgctgccg gaggaagtgg tttcccattt
     7081 cgggccgcgc tgcagcatcg tgccgctgga gagttcccgc accgacttcc tgccgcccaa
     7141 attcgccgct cagggtgtgg actgcgcggc ggaaacggcc taccacatcg tcaagcagga
     7201 atagcgttcc ccatcccggg ccggtcgacg gtcggcgagc agcagttttg ttgccgggtg
     7261 gggatcaatt ttctcttttc aagggctttt aaattttccg gcagcgccca tttatcggga
     7321 atcgttgttt gaactgagtg gttaggagcc aactgaaatg agcattgtcc gatggaaccc
     7381 ctggcgggaa ttcgacgatt tcttcaccaa cgtggccgcg gtgcccagtg agcacatcgc
     7441 tcgttcggag tggctgccgc ccgtcg
//
LOCUS       DBNHKV010000445         4332 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_913690, whole genome shotgun sequence.
ACCESSION   DBNHKV010000445 DBNHKV010000000
VERSION     DBNHKV010000445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_913690"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="AC16PP_14570"
     CDS             <1..561
                     /locus_tag="AC16PP_14570"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR6482901.1"
                     /translation="IRLMPGQPAQQIHRDRWAWGKYLKDVEPQFNTIWALSDFTQANG
                     ATQVVPGSIDWPDDRVAKPDEICQAVMSAGSALLYTGSVFHGGGENRSDGDRIGMNLT
                     YSLGWLRQEENQYLSCPPEIARELPEEMQNLLGYQLGSYALGYYTPPLPPGEGPEAVG
                     PQWAVGRRDSHEALGDAELLEAVINM"
     gene            complement(647..1003)
                     /locus_tag="AC16PP_14575"
     CDS             complement(647..1003)
                     /locus_tag="AC16PP_14575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6482902.1"
                     /translation="MTVIIAGIMELEDPSQVKEMLLAARPHIEGALQEEGCIEYAWTE
                     DHLNPGRVHVYEEWTSSETLEAHLQSHWYRDMGAHLRQYPRKPPTRVIKKYRVDLEEP
                     VYDSEGVARGHFFTES"
     gene            1193..2053
                     /locus_tag="AC16PP_14580"
     CDS             1193..2053
                     /locus_tag="AC16PP_14580"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6482903.1"
                     /translation="MSGGRLEGKVAIVTAAGRGIGRGIALRLAEAGADLVVNSYGEDT
                     TRSTAEAVEATGRKALQVPGDITDPEKMLELTERALETYGQIDVLVNNVGAGPKSAIE
                     PEPGPLGPVAALWDALYQQNLRPVVLMTEAVLPHLIARKSGRIIHISSIAGRTSLSAP
                     MLTHFVHNSYGAMKAGLINYTQTLAELVGPHNINVNAICPGIVWTDAWRANAERAVTA
                     LPEFAGQDPREWFEGIARGDYPEIFDRTPLRREQTVEDIGNAAVFLASDDAMNITGQS
                     LMVDGGMVKV"
     gene            complement(2058..2507)
                     /locus_tag="AC16PP_14585"
     CDS             complement(2058..2507)
                     /locus_tag="AC16PP_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6482904.1"
                     /translation="MSENNAVEETKAVLGRRGHVTTSWDELETLQLSTGIETTMFRIG
                     MPDDPSAPTVFKVYYPPGCKVEAHTHECDYSEIVLEGSQKVGNEWLHPGDIRVGMANR
                     GYGPLVAGDEGATVLVIFADGQWPAVTIGRNDGSSLGVDELLKTYGR"
     gene            2626..3399
                     /locus_tag="AC16PP_14590"
     CDS             2626..3399
                     /locus_tag="AC16PP_14590"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6482905.1"
                     /translation="MSRTVQDLFDMSGHVAVVTGGGTGIGAGIARAFHESGAAVILAA
                     RRTENIERIAKEIRDDGGSAIAVTTDVTDDDAVDALARAAVEEFGRLTDWVNNAGGSP
                     MRMPLSELPREEWDRTIALNLTSIYVGCVTAARHMEHGSIINITSGAGTGPVPGSAHY
                     GASKAGTNSLTWTLAAEFAPRIRVNAVAPGAIPTEVMLKAIGRTEDELDKVLEEWNIP
                     LGRLGTPQDIGAACVYLASDAASWVSGEIIRVGGGARPR"
     gene            3419..>4332
                     /locus_tag="AC16PP_14595"
     CDS             3419..>4332
                     /locus_tag="AC16PP_14595"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6482906.1"
                     /translation="MYPGKHVTEHAERPAIIMASTGEVITYKELEARTNQLAHLLRDQ
                     GLKRLDHYAIFMENNSRYVEACGAGERSGLYYTCINSYLTPEEVAYIVNNSESRVLIT
                     SREKAEVAKAALADCPRVALCLVVDGDDDARVVNYESAIERFPSTPIPDESLGTAMLY
                     SSGTTGRPKGIIRPLPEAPPEQDLPLFQFINGIWRFREGMIYLSPAPLYHSAPQGSVN
                     LTIRRGGTAVIMERFDPEQFLELVERYRITHSQLVPTMFSRMLKLPEEIRARADVSSL
                     EIAIHAAAPCPVQVKEAMIDWWGPIIYE"
BASE COUNT          792 a         1480 c         1384 g          676 t
ORIGIN      
        1 atccgcctca tgcccgggca gccggcccag cagattcacc gtgaccgctg ggcctggggc
       61 aagtacctca aggacgtgga gccccagttc aacaccatct gggcgctgtc cgatttcacc
      121 caagccaacg gcgcaaccca ggtggtgccg ggcagcatcg actggccgga cgatcgggtg
      181 gcgaaacccg atgagatctg tcaggccgtc atgagcgccg gatcggccct gctctacacg
      241 ggatcggtct tccacggcgg cggcgagaac cgctccgacg gggatcgcat cggcatgaac
      301 ctgacctact ccctggggtg gctgcgacag gaggagaatc agtatctctc ctgcccaccc
      361 gagatcgccc gggaactgcc cgaggagatg cagaacctgc tgggctacca gctcggcagc
      421 tacgccctgg ggtactacac cccgccgctg cctcccggtg aagggcccga ggccgttggc
      481 ccgcagtggg ccgtggggcg gcgcgacagc cacgaggccc tgggcgacgc ggagctgctg
      541 gaagcggtca tcaacatgta gcgtcggccg ccagtgacgg cggtgcgctg ggaaaaacgg
      601 gcgcacgccc tcccgatcag gtctggaacc agatcaattc ccgatcctag ctttcggtga
      661 agaagtgccc gcgcgcgacg ccttcggaat cgtagacggg ctcttccagg tcgacgcggt
      721 atttcttgat gacccgggtg ggcggcttgc gcggatactg gcgcagatga gcccccatat
      781 cacgatacca gtggctctgc agatgggcct ccagcgtctc agaggacgtc cactcctcgt
      841 agacgtgcac gcgcccgggg ttcagatggt cctcggtcca cgcgtactcg atgcagccct
      901 cctcctgcag cgcgccttca atgtgcgggc gcgcggccag cagcatctcc ttcacctggc
      961 tcggatcctc gagctccatg atccccgcaa tgatcaccgt catcgatcgt tctcccgcag
     1021 ttgactgatc ccgaaattgc catgcaaagt ctatctcaat cacctcggca tgcgagggaa
     1081 ccgccaccag ggagcaaagg ctgatgggag aggagctgaa cggtcaacgc agacagcggg
     1141 caatcataga gtcgagccgc cagacgccgg gacgggacgg ggggaccgcg cgatgagcgg
     1201 tggcaggctg gaaggcaaag ttgccatcgt caccgccgcc ggcaggggaa tcgggcgagg
     1261 catcgccctg cgcctggccg aggccggcgc cgatctggtg gtgaactcat acggtgagga
     1321 caccacgcga tcaacggccg aggccgtgga ggccaccggc cgcaaggcgt tgcaggtccc
     1381 gggggacatc accgatccgg aaaagatgct cgagctcacc gagcgggccc tggagaccta
     1441 cggtcaaatc gacgtgctgg tcaacaacgt gggcgccggg cccaagtcgg ccatcgagcc
     1501 cgagcccggc cccctgggcc cggtggccgc gctgtgggac gccctctatc agcagaatct
     1561 tcggcccgtg gtgctgatga ccgaggcggt tctacctcac ctcatcgccc ggaagagcgg
     1621 caggatcatt cacatctcgt ccatcgccgg gcgcacctcg ctgtcggcgc cgatgctgac
     1681 ccacttcgtg cacaactcct acggcgccat gaaggccggg ctcatcaact acacccagac
     1741 cctggccgag ctcgtcggtc cacacaacat caacgtcaac gccatctgtc ccggcatcgt
     1801 ctggacggac gcctggcggg ccaacgcgga gcgggccgtc acggcgctgc ccgagttcgc
     1861 cggccaggat ccgcgggaat ggttcgaggg catcgcccgc ggcgactatc cggagatctt
     1921 cgaccgaacg ccgctgcgcc gggagcagac ggtcgaggac atcggcaacg ccgccgtctt
     1981 cctggcatcg gacgacgcga tgaacatcac cggccagtcc ctgatggtgg acggcggcat
     2041 ggtcaaggtc tgagggctca gcggccgtag gttttcagga gctcgtcgac tcccagggag
     2101 ctgccgtcgt tgcggccgat ggtgaccgcc ggccactgcc cgtcggcaaa tatcaccagc
     2161 accgtggcgc cctcgtcccc ggccaccagc gggccgtagc cccggtttgc catgcctacc
     2221 cggatgtccc ccgggtgcag ccactcgttg cccaccttct gagatccctc cagcacgatc
     2281 tcggaatagt cacactcgtg ggtgtgcgcc tcgaccttgc agcccggcgg gtagtagacc
     2341 ttgaataccg tcggggcgga cggatcgtca ggcatcccga tcctgaacat cgtggtttcg
     2401 atccccgtgg aaagctgcag cgtttccagc tcgtcccagg acgtggtgac gtggccccgg
     2461 cgtcccagca cggccttggt ttcctcaacg gcgttgttct cgctcactga tcgctccagc
     2521 tcaagaattc ccgaacgtta gcaagaccgg cacgggcact caacgatcgc ctgaagcggc
     2581 cggtgtgtga taatcccagg caccaagggg gaggagagcg tgggcatgag cagaaccgtt
     2641 caggacctgt tcgacatgag cgggcacgtg gccgtcgtca ccgggggcgg caccggcatt
     2701 ggtgccggca tcgcccgggc cttccacgaa tccggcgctg ccgtgatcct cgccgcgcgc
     2761 cggaccgaga acatcgagcg catcgcgaag gagattcgtg acgacggcgg cagcgccatc
     2821 gccgtcacca ccgacgtcac cgacgacgat gccgtcgacg cgctggcccg cgctgccgtg
     2881 gaggagttcg gccggctcac cgactgggtc aacaacgccg ggggctcgcc catgcggatg
     2941 ccgctcagcg agcttccgcg ggaggagtgg gacaggacca tcgccttgaa tctcacctcg
     3001 atctacgtgg gttgcgtcac ggcagcgcgg cacatggagc atgggtcgat catcaacatc
     3061 acctctgggg ccggaaccgg cccggtgccg ggcagcgccc actacggggc ctccaaggcc
     3121 ggcaccaact cccttacctg gacgctggcc gccgagttcg cgccccgcat ccgggtcaac
     3181 gcggtagcgc cgggggccat ccccaccgag gtcatgctca aggccatcgg caggaccgag
     3241 gacgagctcg acaaggtgct ggaggagtgg aacattccgc tggggcgtct gggtaccccc
     3301 caggacatcg gcgctgcctg cgtctacctg gcctcggacg cggccagctg ggtgagcggc
     3361 gagatcattc gcgtgggcgg cggcgcccgc ccgcgctagc cgtcgaggga ggttcgtcat
     3421 gtacccgggc aagcacgtca cggagcacgc cgaaaggccc gccatcatca tggcctcgac
     3481 cggagaggtc atcacgtaca aggagctcga agcacgtacc aaccagctgg cccatctcct
     3541 gcgcgaccaa ggcctcaaaa ggctcgacca ctacgccatc ttcatggaga acaacagccg
     3601 ctacgtggaa gcctgcggcg ccggcgagcg atcgggcctc tactacacgt gcatcaactc
     3661 gtacctcacg ccggaggaag tggcctacat cgtcaacaac agcgaatccc gagttctcat
     3721 cacttccagg gagaaggcgg aggtggcgaa ggcggctctc gcggactgcc cccgggtagc
     3781 gctgtgcctg gtcgtcgacg gcgacgacga cgccagggtg gtcaactacg aaagcgcaat
     3841 cgaaagattt ccttcgacgc cgattcccga cgagagcctc ggcacggcca tgctctactc
     3901 ctccggcacc accgggcgtc ccaagggcat catccggccc ctgccggaag caccgccgga
     3961 gcaggatctg ccgctcttcc agttcatcaa cggcatctgg cgctttcgcg aaggcatgat
     4021 ctacctctcg cccgcacccc tctaccactc ggccccccag ggctcggtca acctaaccat
     4081 ccggcggggc ggcaccgccg tgatcatgga gcgcttcgac cccgagcagt tcctggaact
     4141 ggtcgaacgt taccgcataa cccacagcca gctcgtgccg acgatgttca gccgcatgct
     4201 gaagctgccc gaggagatcc gcgcccgggc cgatgtctcg tccctggaga tcgccatcca
     4261 cgcggcagcg ccatgcccgg tgcaggtcaa ggaagcgatg atcgactggt ggggccccat
     4321 catctacgag ta
//
LOCUS       DBNHKV010000446        11814 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_415745, whole genome shotgun sequence.
ACCESSION   DBNHKV010000446 DBNHKV010000000
VERSION     DBNHKV010000446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11814)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11814)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11814
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_415745"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="AC16PP_14600"
     CDS             complement(<1..669)
                     /locus_tag="AC16PP_14600"
                     /inference="COORDINATES: protein motif:HMM:NF016356.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HIR6482907.1"
                     /translation="MADALPERPVPPLPIAISECLLGSKVRYDGSSSRSSFPHTLLDG
                     LFTYRGICPEVGIGMGTPREPIRLVGKPDDARVVGVKDPTMDVTERLIEFGRETAASL
                     TDVCGYVLMKNSPSCGLFRVKVYPVTDGEVTGAPDPRGRGAHAAAITAALPDLPAEEN
                     GRLHDPVLRENFVTRVFTYGHWQRCFGSADDLTAAKLVGFHSRYKYLLMAHSVPDYEA
                     AGRLL"
     gene            complement(662..1612)
                     /locus_tag="AC16PP_14605"
     CDS             complement(662..1612)
                     /locus_tag="AC16PP_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6482908.1"
                     /translation="MSTRQMLTDSAERIFADHCTKALLDGAQEGEFPGDLWAVLKENG
                     FAELATADSGFELGDAFAVLKVAGRHAAPLPLAEIMLGNRWLGSADRLVSVGLVDGDV
                     VRDVPWGRRADAVIALDPSGGPPSVLEVTQVDSTGSNLAGEARDVLALTAALPLGSNE
                     PGLELLALARVAQMAGALERILELTLRYANEREQFGRPIARFQAIQHNLAVMAAEVAA
                     AVRIADAAVDALGGARFEFEVAAAKSRVGEAAGVCAESAHQIHGAMGFTHEHQLHHFT
                     RRVWAWREEFGNEIYWRRRLGDHIAGLGADAVWDFLATRG"
     gene            complement(1612..2757)
                     /locus_tag="AC16PP_14610"
     CDS             complement(1612..2757)
                     /locus_tag="AC16PP_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020296375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6482909.1"
                     /translation="MLQFDSMELPEETVALRSEVRSFLDSEHSHLTRRNSDFGSGHDP
                     EFSRKLGAKGWIGMAWPKAYGGGERTFFERYVVTEELLSAGAPVGAHWIADRQSGPLL
                     LRFGTEAQREKYIPPITRGESYFSIGMSEPNSGSDLASVRTTASKVADGWVVNGTKLW
                     STDAHRNNYMIALVRTEPPSENRHAGLSQVIIDLHDDGAQIRPIKNIAGHEDFNEVVF
                     EDVLIPEDRLVGEPGNGWEQVTSELAYERAGPERFLSSFRVFVELVRAVGKDPLPHEA
                     DAIGRIASHLMALRRMSISVAGMLEAGKSPQVEAALVKELGNNFEKLLPEIARLAAPD
                     GLSGDEERLRSFRSTFTDTLLGSPSFTIRGGTREILRGVIARGLGLR"
     gene            2978..3382
                     /locus_tag="AC16PP_14615"
     CDS             2978..3382
                     /locus_tag="AC16PP_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482910.1"
                     /translation="MRKEDLQAAFGPVLDEVAERSQVADAFLDKDLYRIYIATLWANV
                     VLDPSDVGIGEADLETVHDLVNEQIGDVLGSGTDIRECFRFVNSKAGERAMGAAKLTQ
                     RHKELLLYFSSMILDPDGHRRWMENVRNRLER"
     gene            complement(3360..3773)
                     /locus_tag="AC16PP_14620"
     CDS             complement(3360..3773)
                     /locus_tag="AC16PP_14620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR6482911.1"
                     /translation="MNYTLSVSTGRQGLYDITHKVVECLGRSGISEGLVTVFVRHTSA
                     SLLIQENADPSAREDLESWLNRLVPEVDELYTHRAEGPDDMPAHIKSALTATSLSIPV
                     QDGAMLLGTWQGVFLWEHRHHHDRREIIVSVLDGS"
     gene            3918..7199
                     /locus_tag="AC16PP_14625"
     CDS             3918..7199
                     /locus_tag="AC16PP_14625"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HIR6482912.1"
                     /translation="MGIYHHNPLTVGTLWALALLWAAVPLLMARRANRSTLDEAARNA
                     LESSPCGSAVIDATGRIVWVNAAFAAQIDRGSDSMAGTLLSDVAREDLWAELESRREE
                     LDRGVVIDVSSCLVRADGEEVWVAGHVRAMEPESGRLCLQLVDVSSALGAQDSLAKSV
                     AEFRQALESSNDLILMVSKHGKINYANPVATSCLAGDADLNEAVITDLVELNDRERFI
                     TALKNSRKKGEVAELSGLHLLRPDRDSDFPVAARIAGLRTPDGVGGAIVTCRLMDDQI
                     ASERELRSSEARFSRIFHSSPDAILIVRNADSTILDFNAGFIRLLGYTREDAIGQAES
                     DMQLWVHEAERERILNNLQTDQEVTAQETKLRRKNGAVVYVELSLRYIEIEGELCILW
                     IGRDVSERAIAEIARRQSEEKFVEVFNRSPDGIAIVRQEDMLICDINEAFVNSSGHQR
                     EDLIGESLFELDVVSESALADAALRLEEDDHFSNLEMTLHTKSGEEIPALVSATTLEV
                     EEEPCFLIIAKDLRDLRATEEQLRASEQRFRGAFENAPIGILLVDMDGKVFQANRFAA
                     ELLSYRNASMRNLHISRLVPDDDRDKYNDLMQRLARGHQATLRTEQRMLCADGAEIWT
                     NMHVVLLRSQDGEPAYGIVQIADITEMKHSQQRMERMAFYDILTDLANRRLFYDRLSQ
                     AIEYTTRNNRLAALLYLDLDQFKRVNDTLGHEAGDTLLRTVAERLSHNVRQEDTVARP
                     GGDEFTVLLYNIGKPEDAGHVAEKILNELRRPITVAGEQLVMTTSIGISIIPSDGTDP
                     KQLMSNADLAMYRAKEQGRNQFQFYSKEMNTNAAHRLKTENELRAALERQEFELYYQP
                     KVRLSDLQIVGVECLLRWHHPERGLLLPGEFIEVAEETGTIVDLGKWVIETACSAATQ
                     LAHEAGEPICTAVNISPRQFRDVELVSTVQRCLAQTGLDPDNLEIEITETMLMDDVEA
                     ASATVCDLHDLGVRLAIDDFGTGYSSLNYLKKFPIDTIKVDRSFVADIPESQDDMAIT
                     AAVIAMARRLHLAVVAEGVQTREQLEFLQQHECEFAQGFLFSQAVPLEALKQLLVPNV
                     RLLRR"
     gene            complement(7174..8967)
                     /locus_tag="AC16PP_14630"
     CDS             complement(7174..8967)
                     /locus_tag="AC16PP_14630"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIR6482913.1"
                     /translation="MSGVLLRWACLVVLLGFGVRVAIAVPLWSDPYVPGTEILLAVLV
                     GVYQDLIALVLLGGPLALAFLAGVRVGRAVLLLLVAVVLVVAFAELFFWWEFESRLNR
                     LVFHYLLFPREVVTFLEDQFYVSLYAIPALLIIAAAYLVVRGRSADQRAPSRWFLVGS
                     VLACVVAVLVASPLEFSSSRRINQMASNGYLGVLHAAVTDVSAWHGHYWNPAQPAPTR
                     GEAPGALGVVEPVWQPLPVKHVVLIVEESFSGEVWDDLALRGTYMPRFSSLADSGVLF
                     ENVYSTGTRTTRGLEALLNGFPPLPGIAVNQRGGFERLPSLARALSLAGFHNVFVYGG
                     WPGFSNFFPYWRALGYEELTSRDDFPEPRFETSWGVADEHLFEAIREHMSRLTQNHER
                     VFLTSLTVSHHRPFDFPAGRVPFPAEERRSEYALAYADWALGQFIEDASGEPWFDDTL
                     FVIAADHGPRISGDAPIPVAGFRVPLLLYAPAHLNPRRVDHLGSLMSFGVTLLELLGL
                     PNDEGLYGDNLLLTEQGLVPMEHEYQVGLLTRDGLTVLHLGGEVSGWRQEDDRLVLDA
                     PDWEVAARAARLFHGAHQWFYGSASAAADGR"
     gene            complement(8964..11528)
                     /locus_tag="AC16PP_14635"
     CDS             complement(8964..11528)
                     /locus_tag="AC16PP_14635"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR6482914.1"
                     /translation="MAGAWLATPLSRWVGGVSRRPWTVLVCIAVATAALGWVALDRFR
                     MNSNLSDLIRQEATWRVDFDRFKEQFPDLVETAVVVVSGTSYRDVEAMTGRLEAALLG
                     RDDLFRAVYAPENDPFFRRHALLYLDEDDLDDMADRLAEAQPMLTAVAEDPSLRGVLE
                     LVGDGIENEPAAGFDTVVRLLAESGEAVLAGGDPTISWTDELFETDGTIHRLIFLKGQ
                     ADFQESLANARIMKELRALIAQLPAIDGVDVRITGEVALAHEEIEAAYAGVQIAGWVA
                     LALLMIILIVGVRSAKIIIATVLLLFVGVTWTSAFAMLTVGEYNTLSIVFLVMFFGLG
                     VDFALHFSLRYQEEINSGEGDALRSLVSATNSVGGAIAICTITTALGFLSFYPTPYKG
                     LADLGVISAGGMLVAGFLTFTLLPALYAVLGDIRPHIVDLPTGDRLVAYLSRHRGPVL
                     AALGLGAALALFLATRAHFDYSVLALKDPRAESVVTLRVLQADDAVTDYALTIMSARQ
                     APEAALEELPTVETVLSPADYVPDGQDDKLFVLEDLQELLWSALEPLRTADPPTGTEL
                     REAAEALTATVAAAQAAAPSSPDVDRLAAVLTGLLERPPQALLIWQRGVITNLLEELG
                     WLREALYVDALAFEDLPEKLRARLVSEEGQRVTVVTPSEDVTAVEALSDFVSSVREVA
                     PTATGRPVIEWGVGDIVVGSFKQALGFATFAILAVLFVMFRNLRDSVLILVPLALAAL
                     FTLAASVVMDMPLNMANILVVPLIFGLGVDNGIHVVDRFHRAGDVGHLMHSSTPRAVM
                     LSTLTTIGTFAALMLSPHQGTASIGLLLTLAVGLLLLLTIFVLPVLLSLLVEPAET"
     gene            complement(11530..>11814)
                     /locus_tag="AC16PP_14640"
     CDS             complement(11530..>11814)
                     /locus_tag="AC16PP_14640"
                     /inference="COORDINATES: protein motif:HMM:NF016236.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HIR6482915.1"
                     /translation="AGPTTIRDAPYSFIRAWWPRLLLGSAYGWWMGIIDLLNTRAEVL
                     AVTEARDASALDPYAFTREAYYQRRKFLIYDGDPPMDDFFDDFEDEDEAF"
BASE COUNT         2298 a         3928 c         3766 g         1822 t
ORIGIN      
        1 caggagcctg cccgccgcct catagtccgg gacgctgtgc gccatgagca ggtacttgta
       61 ccggctgtga aagccgacga gctttgcggc ggtgagatcg tcagccgagc cgaaacagcg
      121 ttgccagtgc ccgtaggtga aaacccgggt cacgaagttt tcccgcagca ctggatcgtg
      181 cagcctgccg ttctcctccg ccggcaggtc gggcagcgcg gcggtaatcg ccgcagcgtg
      241 agcgccgcgg ccccggggat cgggcgcgcc cgtcacctcg ccgtcggtga cagggtaaac
      301 cttgaccctg aacaggccgc agctgggcga gttcttcatg agaacgtaac cgcagacgtc
      361 cgtcagcgac gccgccgtct cccgaccgaa ttcaatgagc cgctcggtga cgtccatcgt
      421 cgggtctttc acccccacga cccgcgcatc gtccggcttg ccaacgagcc tgataggctc
      481 ccgcggcgtg cccatgccga tgcccacctc cggacagatt ccccgatacg tgaacagacc
      541 gtcgagcagc gtgtgcggga acgacgatcg tgacgagctg ccgtcgtagc gtaccttgct
      601 gcccaggagg cactcgctga tggcaatggg caggggcggg accggccgct ctggcagggc
      661 gtcagccacg cgtggcgagg aagtcccaca cggcatcggc acccagcccg gcgatgtggt
      721 ctcccaggcg acggcgccag tagatctcgt tgccgaactc ctcgcgccag gcccagacgc
      781 ggcgggtgaa atggtgcagc tgatgctcgt gggtgaagcc catggcgccg tgaatctgat
      841 gggcgctttc cgcgcaaacg ccggcagcct cgccgaccct ggacttggcg gcggcgacct
      901 cgaactcgaa acgcgcaccc cccagggcat ccacggcggc gtcggcgatg cgcaccgcgg
      961 cggctacctc ggccgccatg acagccagat tgtgctgaat ggcctgaaag cgcgcgatgg
     1021 ggcgaccgaa ctgctcccgt tcgttggcat accgcagggt aagctcgagt atccgttcca
     1081 gcgcccccgc catctgggcc acgcgggcaa gggccaggag ctcgaggccg ggttcgttgc
     1141 tgccaagcgg caaggctgcg gtcagggcga gcacgtcccg agcttcgccg gccaggttgc
     1201 tgcccgtaga gtcgacctgc gtcacctcca gcacggacgg gggtccgccc gaggggtcca
     1261 gcgcgatcac ggcgtccgcc cggcggcccc agggcacgtc tctcaccacg tccccgtcca
     1321 cgagccccac cgacaccaga cgatcggcac tgcccagcca ccgattgccc agcatgatct
     1381 cggccagcgg cagcggcgcg gcgtgacggc ccgccacctt gagcacggcg aaagcatctc
     1441 cgagctcgaa gccgctgtct gccgtcgcaa gctcggcaaa gccgttctcc ttgagcacgg
     1501 cccagaggtc gccgggaaac tctccttcct gtgccccgtc cagcagggcc ttggtgcagt
     1561 gatcggcgaa gatcctttcc gcgctgtccg tgagcatctg acgcgtgctc atcagcgcag
     1621 ccccagcccg cgggcaatca cgccccgaag aatttcccgc gtcccgccgc ggatggtgaa
     1681 cgagggcgac ccgagcagcg tatccgtaaa cgtcgagcgg aacgaacgca gccgctcttc
     1741 gtcgccgctc agaccgtccg gagccgcgag ccgcgcgatc tccggaagaa gcttctcgaa
     1801 gttgttgccc agctccttga ccaaagcagc ttccacctgg gggctcttgc ccgcttcgag
     1861 catgccggcc acggagatgg acatgcgccg cagcgccatc agatgcgaag cgatgcggcc
     1921 aatggcgtca gcttcgtgag gaagcggatc tttacccacg gccctgacca gctccacgaa
     1981 tacgcggaaa ctcgacagga accgttccgg acccgcccgc tcgtaggcga gctcgctcgt
     2041 gacctgctcc cagccgttgc cgggctcgcc gaccagacgg tcctcaggaa tcagaacgtc
     2101 ttcgaagacc acctcgttga agtcctcgtg cccggcgatg ttcttgatcg gacggatctg
     2161 cgccccgtcg tcgtgcagat cgatgatcac ctgggagaga ccggcatgac ggttctcaga
     2221 gggaggctcg gtgcgaacca gcgcgatcat gtagttgttg cgatgcgcgt ccgtggacca
     2281 cagcttcgtc ccgttgacga cccaaccatc cgcgaccttg ctggccgtgg tccgcacgga
     2341 ggcgaggtca gatcccgagt tcggctcgct catgccgatg gagaagtagc tttcgccccg
     2401 ggtgatcggc gggatgtact tctcacgctg ggcctcggtt ccgaagcgca gcagcagcgg
     2461 tccgctctga cggtccgcga tccagtgcgc gcccaccggg gcgcccgcgc tcaagagttc
     2521 ctcggtgacc acgtagcgct cgaaaaacgt tctctccccg ccgccgtagg ccttgggcca
     2581 ggccatgccg atccaaccct tcgcacccag cttccgggaa aattcagggt cgtgaccgga
     2641 accgaaatcc gagtttcggc gcgtaaggtg cgaatgctcg ctgtcgagaa acgagcgcac
     2701 ctcgctgcgc agcgccaccg tttcctcggg cagctccatg gagtcgaatt gcaacaccgg
     2761 gaatcgtcca atgcagataa ggcgtcgcta aattactgac agcccaggtt cattgcaaac
     2821 cgctcgcctc gttgtcgggc aatttcgcgt tgcctacact agcgacatcg attccgcagc
     2881 tgaaaaatcc gatgacgtcg atccccaaac gagagcagag ctcgaataga cctaccccgg
     2941 gcgaacgcgc gctcaaccgg cggcgaccgc cggcagcatg aggaaagagg atctgcaggc
     3001 cgccttcgga ccggtgctcg atgaggtcgc cgagcgttcc caggtagccg acgctttcct
     3061 ggacaaggat ctctacagga tctacatcgc cacgctatgg gcaaacgtcg tcctcgaccc
     3121 ttccgacgtc ggcatcggcg aggcagacct ggaaaccgtc catgatctgg tgaacgagca
     3181 gatcggcgac gtgctcggca gcggcaccga catccgcgag tgtttccggt tcgtcaacag
     3241 caaggccgga gagcgcgcca tgggcgcggc caagctgacc cagcgccaca aggagctgct
     3301 gctgtacttc tcgtccatga ttctcgatcc ggacggtcac cgccgctgga tggagaacgt
     3361 caggaaccgt ctagaacgct gacgatgatc tcgcgccggt catggtggtg ccggtgctcc
     3421 cagagaaaca cgccctgcca ggtccccaac agcatggcac catcctggac cggaattgac
     3481 aagctagtag cggtgagtgc cgatttgatg tgggcgggca tgtcgtccgg tccctctgcc
     3541 cgatgggtat acaactcgtc cacctccggt acgagccggt tcagccacga ttccagatcc
     3601 tcgcgggcgc tcgggtccgc attttcctga atcagcaggc tcgcggaggt gtggcggacg
     3661 aacacagtga ccaagccctc acttatgccg gacctgccta gacattcgac aactttgtgc
     3721 gtgatgtcat agaggccttg acgaccggtg gatacgctca gcgtgtaatt catggaagaa
     3781 atctcagacc acgcgcggac agccgctaag catagcgatc gccgggatcc gggaacaaca
     3841 aagacatgca gggcaaacgt gtacgacagc ttctggtcgc cctaggacca ctcgtatgca
     3901 tctcggcagg gcttgtggtg ggtatttacc atcacaatcc gctgaccgtc ggcacgctct
     3961 gggcgctcgc cctgctatgg gccgccgtgc ccctgctcat ggcgcgccgc gcaaaccgat
     4021 cgacgctgga cgaggcagct cgcaacgctc tggagtcctc gccctgcggc agcgcggtca
     4081 tcgacgcaac gggccgtatc gtctgggtaa acgcggcgtt cgccgcccag atcgaccgag
     4141 gcagcgactc catggcaggc accctcctga gcgacgttgc ccgggaagac ctctgggccg
     4201 agctggaaag ccgccgcgag gagctcgacc gcggcgtcgt gatcgacgtc tcctcctgcc
     4261 ttgtccgcgc cgacggggag gaggtctggg tcgccggcca cgtccgggcc atggagcccg
     4321 aaagcggccg gctctgcctg caactggtgg acgtctcttc cgccctcggc gcccaggact
     4381 ccctggcgaa atccgtggcc gagttccgtc aggccctgga atcgagcaac gacctcatcc
     4441 tgatggtctc caagcacggc aagatcaact acgcgaaccc ggtggccact tcctgccttg
     4501 ccggggacgc ggacctcaac gaggccgtga tcaccgacct cgtcgagctg aacgaccgcg
     4561 agcgattcat caccgccctg aagaactccc gcaagaaggg cgaggtggca gagctctccg
     4621 gtcttcacct gctgcgcccc gaccgcgaca gcgacttccc ggtggcggcg cggatcgccg
     4681 gcctgcgcac gcccgacggc gttggcggag ccatcgtgac ctgccggctg atggacgacc
     4741 agatcgcatc ggagcgcgag ctgcgcagct ccgaagcccg gttctcccga atctttcact
     4801 cgagcccgga cgccatcctc atcgtccgca acgcggactc caccatcctc gatttcaacg
     4861 cgggcttcat ccgcctgctc ggctacacgc gcgaagacgc catcggccag gcggaatcgg
     4921 acatgcagct gtgggtgcac gaggcggagc gggaacgcat tctcaacaac ctccagaccg
     4981 accaggaagt aaccgcccag gagacgaagc tgcgccgcaa gaacggagcg gtggtgtacg
     5041 tggagctgtc cctgcgctac atcgagatcg agggggagct gtgcatcctg tggatcggtc
     5101 gggacgtgtc cgagcgggcc atcgccgaga tcgcccgacg gcagagcgag gagaaattcg
     5161 tcgaggtctt caaccgcagc ccggacggca tcgccatcgt ccgccaggaa gacatgctga
     5221 tctgcgacat caacgaagcg ttcgtgaatt cctcaggcca ccagcgcgag gatctgatcg
     5281 gggagtccct tttcgagctg gacgtggtga gcgagtcggc cctcgcagat gccgccctgc
     5341 gcctggagga ggacgaccac ttctccaacc tggagatgac ccttcacacc aagtccggcg
     5401 aggagattcc cgccctggtg tcggccacca cgctcgaggt cgaagaggaa ccctgcttcc
     5461 tcatcattgc caaggacctt cgcgatctgc gcgctaccga ggaacagctg agggccagtg
     5521 agcagcgctt ccgcggtgct ttcgagaacg cgcccatcgg cattctgctg gtcgacatgg
     5581 acggcaaggt ctttcaggcg aaccggttcg cggcggagct cctgtcgtat cgcaacgcct
     5641 ccatgaggaa cctgcacatc tcgcgtctgg ttcccgatga tgatcgcgac aagtacaacg
     5701 acctgatgca gcggctcgcc agagggcatc aggccacgct gcgcaccgag cagcgcatgc
     5761 tttgtgcgga cggcgcagag atctggacca acatgcacgt ggtgctcctg cgatctcagg
     5821 acggggagcc ggcttacggc atcgtgcaga ttgccgacat aacggagatg aagcacagcc
     5881 agcagcgcat ggagcgcatg gccttctacg acatcctcac cgacctcgcc aaccggcgtc
     5941 tgttctatga ccggctctcc caggccatcg agtacacaac gcgcaacaac cgccttgccg
     6001 ctctgctgta cctggacctc gaccagttca agcgggtgaa cgacaccctc ggccacgagg
     6061 cgggagacac cctcctgcga acggtggccg agcggctgag ccacaacgtg cgccaggagg
     6121 acacggtcgc caggcccggg ggcgatgagt tcaccgttct gctctacaac atcggcaagc
     6181 cggaagacgc ggggcacgtc gccgagaaga ttctcaacga gctgagacga ccgatcacgg
     6241 tggccgggga gcagctggtg atgaccacca gcatcggcat atcgatcatc ccgagcgacg
     6301 gcacggatcc caagcagctc atgtccaacg ccgacctcgc gatgtaccgg gcaaaggaac
     6361 aggggcgcaa tcagttccag ttctacagca aggagatgaa caccaacgcc gcccaccgtc
     6421 tgaagaccga gaacgagctg cgggccgctc tcgagcggca ggagttcgag ctctactacc
     6481 agcccaaggt gcgcctgtcc gatctgcaga tcgtgggcgt ggagtgcctg ctgcgctggc
     6541 atcaccccga gcgcggcctg ctgctgccgg gagagttcat cgaggtcgcc gaggaaaccg
     6601 gcacgatcgt cgatctcggc aagtgggtca tcgagacggc ctgctccgcc gccacccagc
     6661 tggctcatga ggcaggcgag ccgatctgca cggcggtgaa catctcgccg cgccagttcc
     6721 gggacgtcga gctggtgagc accgtgcagc gctgcctggc gcagacaggc ctggaccccg
     6781 acaatctgga gatcgagatc acggaaacca tgctgatgga cgacgtggag gcggcatcgg
     6841 ccacggtgtg cgacctccac gacctgggcg tgcggctggc catcgacgat ttcggtaccg
     6901 ggtattcttc cctgaactac ctgaagaaat ttcccatcga caccatcaag gtcgatcgca
     6961 gcttcgtggc cgacatcccc gagagccagg atgacatggc catcaccgcg gccgtgatcg
     7021 ccatggcgcg acggctgcac ctggccgtgg tcgccgaagg cgtgcagacc cgcgagcagc
     7081 tcgagttcct gcagcagcac gagtgcgaat tcgcccaggg gtttcttttc agccaggccg
     7141 tcccgctgga agctttgaaa cagctgctgg tgcctaacgt ccgtctgctg cggcgctagc
     7201 gctaccgtag aaccactgat gggcgccgtg gaagaggcgc gccgcccgtg ccgccacttc
     7261 ccaatcaggc gcatcgagca cgagccggtc gtcttcctgc cgccaccccg agacctcacc
     7321 gccaaggtgc aacacggtca ggccatcccg ggtgagcaga cccacctggt attcatgctc
     7381 catgggcacg aggccctgtt cggtgagcag aagattgtct ccgtagaggc cttcgtcgtt
     7441 cggaagcccc agcagctcga gcagcgtgac gccgaaactc atcagcgacc ccagatggtc
     7501 gacccgacgc gggttcaggt gagctggcgc gtacagcagc agcggaacgc gaaaacccgc
     7561 gaccggaatg ggcgcgtcgc cgctgatgcg ggggccgtgg tcggcagcga tgacgaacag
     7621 ggtgtcgtcg aaccagggtt ccccgctcgc atcttcaatg aactggccca gggcccagtc
     7681 cgcataggcc agcgcgtact ccgagcggcg ttcctcggcc ggaaacggga cccgccctgc
     7741 cggaaagtcg aacgggcgat gatggctgac cgtcagcgac gtcaggaaca cccgctcgtg
     7801 gttctgggta agacggctca tgtgctcacg gatggcttcg aacagatgct cgtcggccac
     7861 gccccagctg gtttcgaaac gcggctccgg gaagtcgtcg cggctggtca gctcttcgta
     7921 tcccagggca cgccagtagg ggaaaaagtt cgagaatccg ggccatccgc cgtaaacgaa
     7981 gacgttgtga aacccggcaa ggctcaatgc ccgcgccaag gagggcagcc gttcgaaccc
     8041 gccgcgctga ttgacggcga tgcccggcag gggcggaaag ccgttcagca gtgcctccaa
     8101 cccccgggtc gtgcgggttc ccgtcgagta gacgttctcg aaaagcacgc cggaatcggc
     8161 gagtgaagag aaacgtggca tgtaggtccc acgaagcgcc aaatcgtccc aaacctcgcc
     8221 ggagaaggat tcctccacga tcagcaccac atgcttaacc gggagcggct gccagaccgg
     8281 ctccaccacc cccagagcgc caggtgcttc cccacgtgtc ggggcgggct gcgccgggtt
     8341 ccagtagtga ccgtgccagg cagaaacgtc ggtcacggct gcgtggagca cgcccaggta
     8401 accattgctc gccatctggt tgatgcgccg tgacgagctg aactccagcg gcgaggccac
     8461 cagcaccgcc accacgcagg cgagcaccga gccgacgaga aaccagcggg aaggcgcgcg
     8521 ctggtccgcc gaacgcccgc gcacgacgag gtaggccgct gcgatgatga gcagcgcagg
     8581 aatggcgtac aggctcacgt agaactgatc ctctagaaag gtcaccacct cccggggaaa
     8641 cagcagatag tggaagacga ggcgattgag ccggctttcg aactcccacc agaaaaacaa
     8701 ctcggcaaac gcaaccacga gcaccacggc cacgaggagc aggagaaccg cacgccccac
     8761 gcggacgccc gccagaaagg ctagcgccag agggccgccg agcagaacca gcgcgatcag
     8821 atcctggtaa acgccgacca gaacggcgag aagtatctcg gtgcccggca cgtagggatc
     8881 gctccacagc gggaccgcaa tggccaccct gacgccgaaa ccgagcagga ccaccaggca
     8941 ggcccagcgc agaagcactc cactcatgtc tcggcgggct cgacgagcag agacagaagc
     9001 accggcagaa cgaatatggt cagaagcaga agaaggccga ccgcgagggt cagcagcagc
     9061 ccgatggatg ccgttccctg gtgcggcgag agcatgaggg ccgcgaaagt cccgatggtg
     9121 gtcagcgtcg aaagcatgac ggcccggggc gtgctcgagt gcatcaggtg gccgacatcg
     9181 cccgcgcgat gaaatcggtc gaccacgtgt atgccgttgt ccacgccaag cccgaagatg
     9241 agcggcacca ccagaatgtt ggccatgttc aggggcatgt ccatcacgac gcttgccgcc
     9301 agggtaaaca gcgcggcaag cgccaggggc acgaggatga gcacgctgtc gcgcaggttg
     9361 cggaacatga cgaacagcac ggccaggatg gcgaaggtgg caaagccgag cgcctgcttg
     9421 aacgagccga ccacgatgtc gccgacgccc cactcgatga cgggccgtcc ggtggcggta
     9481 ggcgcgacct cgcgcacgct ggagacgaag tcgctcagcg cctcgacggc ggtgacatcc
     9541 tccgaaggcg tcaccacggt gacccgctgt ccctcctcgc tgaccaggcg ggcgcgcagc
     9601 ttctccggca ggtcctcgaa ggcgagcgca tccacgtaca gggcttcacg aagccaacca
     9661 agctcttcca aaaggttggt aatgacgccc cgctgccaga tcagcagcgc ctggggcggc
     9721 cgttcgagga gcccggtcag cacggcggcc aaccggtcca cgtccggcga ggacggcgcg
     9781 gcggcctggg ctgcggccac cgtcgcggtc agggcctccg cggcctcacg cagttcagtt
     9841 cccgtgggcg gatcggcggt tctaagcggc tcgagcgcgc tccatagcaa ctcctgcaga
     9901 tcctccagga cgaacagctt gtcgtcctgc ccgtcgggaa cgtaatccgc cggtgacagc
     9961 acggtctcca ccgtcggcag ttcttccagc gccgcctcgg gcgcctgccg cgcggacatg
    10021 atggtgagcg cgtagtccgt cacagcgtcg tccgcctgca ggacgcgcag cgtcacgacg
    10081 gactccgccc tggggtcctt cagcgcgaga acgctgtagt cgaagtgcgc gcgggtcgcg
    10141 agaaagagag cgagggctgc gcccaagccg agcgccgcga gcacgggtcc tcgatgtcgc
    10201 gagaggtagg cgaccaggcg gtcacccgtg ggcaggtcga cgatatgggg cctgatgtcc
    10261 ccgagcacgg cgtaaagtgc cggcagcagg gtgaaggtaa gaaacccggc cacgagcatg
    10321 ccgcccgcgg agatcacgcc caggtcggcc aggcccttgt acggcgtggg atagaagctc
    10381 aagaacccca gcgcggtggt gatggtgcag atggcgatgg ctccaccgac gctgttcgtg
    10441 gccgacacca gcgagcgcag ggcatcgccc tctcccgaat tgatttcttc ctggtaacgc
    10501 agcgagaaat gcagcgcgaa gtcgacgccc aggccgaaga acatgacgag gaagacgatg
    10561 gagagcgtgt tgtattcgcc caccgtgagc attgcgaacg ccgaggtcca ggtcacgccg
    10621 acgaagagca ggagcaccgt ggcaatgatg atcttcgccg agcgcacgcc gacgatcaga
    10681 atgatcatca gcagggccag cgccacccac ccggcgatct ggacgccggc gtaggcggcc
    10741 tcgatctcct cgtgagcaag agccacctcg ccggtgatcc gcacgtcgac cccgtcgatg
    10801 gccggcagct gggcaatgag ggctctgagc tccttcatga tacgggcgtt ggccagagac
    10861 tcttgaaaat ccgcctgacc tttgagaaag atcagccggt ggatcgtccc gtcggtctcg
    10921 aagagctcgt ccgtccagga gatcgtcgga tcccctcccg cgaggaccgc ctcgcccgat
    10981 tcggcgagca gacggaccac cgtgtcgaac ccggccgcgg gctcgttctc gatcccgtcg
    11041 ccgacgagct cgagcacgcc ccgcaggctc ggatcctcgg ccacggcggt gagcatcggc
    11101 tgagcctccg ccagccggtc ggccatgtcg tcgagatcgt cctcgtcgag gtacaacagc
    11161 gcgtggcggc ggaagaacgg atcgttctcc ggcgcgtaca cggcgcgaaa aagatcgtcc
    11221 cggccgagca gtgcagcctc cagccgcccg gtcatcgcct cgacgtcccg atacgaggtt
    11281 cccgaaacca ccacgacggc ggtttcgacg aggtcgggga actgctcctt gaagcggtcg
    11341 aaatccaccc gccacgtggc ttcctggcga ataaggtcgc tcaggttcga gttcatccgg
    11401 aatcgatcga gggcaaccca gccaagcgcg gcggtagcca cagcgatgca gaccagcacc
    11461 gtccagggcc ggcgcgaaac cccgcccacc cagcgcgaca gcggcgttgc caaccaggcg
    11521 ccggccattc tagaaggctt cgtcttcgtc ttcgaagtcg tcgaagaagt cgtccatggg
    11581 cggatcgccg tcgtaaatca ggaacttgcg gcgctgatag taggcttcac gggtaaaggc
    11641 gtagggatcg agcgccgagg cgtctctcgc ttcggtcacg gccagcacct ccgcacgggt
    11701 attgagcagg tcgatgatgc ccatccacca gccgtaggcg ctgccgagca gcaggcgcgg
    11761 ccaccaggcc cggatgaagg aataaggcgc gtcccgaatc gtggtcggcc cggc
//
LOCUS       DBNHKV010000447         4839 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_415964, whole genome shotgun sequence.
ACCESSION   DBNHKV010000447 DBNHKV010000000
VERSION     DBNHKV010000447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_415964"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="AC16PP_14645"
     CDS             complement(<1..1526)
                     /locus_tag="AC16PP_14645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6482916.1"
                     /translation="MSTAKEPHGPAGGVSRPDEQAVPSALPRAFGASVKRKEDRRLIT
                     GRGNYTDDLLLPDQAFAAFVRSPHAHAAINSIDAEAAREMPGVVAVLTGEEFAASGLG
                     PLMCGWMIHSRDGEPMKVGEHPALASDKVRYVGDAVALVVADSLQAARDAAECVYVDY
                     DDLPAVADVVSAGSVDAPELHSGIAGNEAFDWEIGDADAVAAAFARAAHVTRLRLINN
                     RLVPNAMEPRSLNAQFDVGTGQHTLHITSQNPHGLRMTLAAVIGFAPEHKIRVISEDV
                     GGGFGSKAFNYAEEVACLWAARETGRPVKWAADRSEAFLTDAHGRDHVTTAELALDAD
                     GRFLALKVHTQANVGAYLSTFGSLIPTYVYAPLLSGQYEIPAIHANVIARYTNTAPVD
                     AYRGAGRPEAGYVVERLVEEAARELGIDSAELRRRNFVRSFPYVTPVEMEYDTGDFQA
                     TLDQALQLIDRDGFPARRAASEEQGKLRGLGIGAYIEAAGIGPSAKLGKLGSGAGLWE
                     S"
     gene            complement(1660..2757)
                     /locus_tag="AC16PP_14650"
     CDS             complement(1660..2757)
                     /locus_tag="AC16PP_14650"
                     /inference="COORDINATES: protein motif:HMM:NF053593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3169 family protein"
                     /protein_id="HIR6482917.1"
                     /translation="MLTKADDYPIHQTPEPIAYSGTDRNFYDRYFFNGYQREGELFFA
                     AALGVYPHLNVMDAAFSILVGGRQYNVQASKVLHMERLNTQVGPIEVEVIEPLNVLRV
                     SCADPANGVAADVCFRARVPAVEEPRFTRRQGSLTFMDYTRLTQNGSYEGWLEVDGQR
                     YELTHDETWGTRDRSWGIRSVGARDPQPNPYAGEPQFYWLWAPLNFPSTNNYYHLNAD
                     REGRPWNTMGRLADPERSEEMAAAASSIRHRSGTRLAESATLEYVRENGEEVTIELQP
                     TQTFYMRGLGYMHPEWGHGLFHGELATNYEVYETAEIADADPGYFHVQAICDAQLTDA
                     RGSERGKGVLEQLVIGAYEPYGFRELFDVAP"
     gene            2794..3129
                     /locus_tag="AC16PP_14655"
     CDS             2794..3129
                     /locus_tag="AC16PP_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482918.1"
                     /translation="MDWERYKQLCDQPNVFSRWMLNQTIELIGESPVADTLAGVLGSF
                     PLEKPDDHRGGPATDMFVLELESGVRRAVVELVLLAREAGAATEATSHRGLGGFVEAW
                     REYLNWTRG"
     gene            3208..3594
                     /locus_tag="AC16PP_14660"
     CDS             3208..3594
                     /locus_tag="AC16PP_14660"
                     /inference="COORDINATES: protein motif:HMM:NF019473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HIR6482919.1"
                     /translation="MSDDKAVVLELIDGFNSIDLDRIVACFTEDAVYDNIPMQAVQGT
                     EAIRASLEGFMGAASEVQWDLLNVVGEGGVVLTERIDKFKVNGTWIELPVMGTFEVAD
                     GKIKAWRDYFDLAQFQTQFAKATGAQ"
     gene            3611..>4839
                     /locus_tag="AC16PP_14665"
     CDS             3611..>4839
                     /locus_tag="AC16PP_14665"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR6482920.1"
                     /translation="MFRRLLIANRGAIARRVVRACNDLGIESVAVYSEADAHAPYLSE
                     ASRAVGLPGVSAGETYLNTAALLEAVRASGADAVHPGYGFLAENADFARAVAAAGATF
                     VGPDPKWLEVMGDKVAARRLLADHGFPVFPGSGGLSSLDEALSAAAELSYPVLLKPAA
                     GGGGIGMRLVRDEAELARAFASAQRLAEQAFGQGGIYLERAIDKPRHVEFQILADGAG
                     GALHLYERECSIQRRHQKIIEEAPAVGLPREELDALAARAEAVMAEIGYDNVGTLETL
                     CTQQGEFGFLEMNARIQVEHAVTEAVTGLDLVGLQIRLAAGAALPERPGLCGHAVEAR
                     IYAEDPRTLLPDTGVLRVFRAPRMHRVRVETGYQEGQAVTPFYDAMLAKVIGWGETRE
                     LAIGRTLVGLKGFEIRGV"
BASE COUNT          788 a         1535 c         1681 g          835 t
ORIGIN      
        1 gactcccaca gccccgcgcc cgatcccagc ttgcccagct tggccgaggg accgatcccg
       61 gctgcctcga tgtaggcgcc gatgcccagg ccgcgcagct tgccctgctc ttcggatgca
      121 gcccgccggg caggaaaacc atcacggtcg atgagctgca gcgcctggtc cagggtcgcc
      181 tggaagtcgc cggtgtcgta ctccatctcc accggggtga cgtaaggaaa cgaccgcacg
      241 aagttgcggc gccgcagctc ggcagaatcg atgccgagtt cccgtgccgc ctcctccacc
      301 agccgctcga ccacgtagcc cgcctccggg cggccggcgc cgcggtaggc gtccacgggt
      361 gccgtattcg tgtagcgggc gatgacgttg gcgtgaattg ccgggatctc gtactgcccc
      421 gacaacaagg gggcgtagac gtaggtcggg atcagcgagc cgaacgtgga caggtaggcg
      481 ccgacattgg cctgagtgtg caccttcaag gccagaaaac ggccgtcggc atccagggcg
      541 agctcggcgg tggtgacgtg atcgcggccg tgggcgtcgg tcagaaacgc ctctgaccga
      601 tcggccgccc acttcacggg cctgccggtc tcgcgggccg cccaaaggca cgccacttct
      661 tccgcgtagt tgaatgcttt ggatccgaag ccgccgccca cgtcctcgga gatgaccctg
      721 atcttgtgct cgggggcgaa gccgatcacg gctgccagcg tcattctcag gccgtggggg
      781 ttctggctgg taatgtgcag cgtgtgctgg ccggttccaa catcgaactg cgcgttgagg
      841 gagcgcggct ccatggcgtt cggcaccagg cggttgttta tcagccgcag ccgggtgacg
      901 tgggcagccc tggcgaaagc ggctgctacc gcgtcggcat cgcctatttc ccagtcgaac
      961 gcttcgttgc ctgcaatccc cgaatgaagc tccggcgcat cgacggaccc tgccgaaacc
     1021 acgtcggcga ccgccggcag gtcgtcgtag tcgacgtaca cgcactcagc ggcgtcccgt
     1081 gcggcttgaa gcgagtcggc aaccaccagg gcgaccgcgt cgccgacgta gcggaccttg
     1141 tccgacgcca gcgccgggtg ctcgcctacc ttcatgggct cgccgtcgcg ggagtggatc
     1201 atccagccgc acatcagcgg gccgagaccg ctggccgcga attcctcgcc cgtcagcacg
     1261 gcgaccacgc cgggcatctc cctggccgct tccgcgtcga tggaattgat agcagcgtga
     1321 gcgtgggggg atcgcacgaa ggcggcgaag gcctgatcgg gcaggagcag gtcgtcggtg
     1381 tagttcccgc gcccggtgat caggcgccgg tcttccttgc gcttcaccga cgcgccgaac
     1441 gcccgcggaa gggcgctcgg aacggcttgc tcatcgggtc gcgatacccc gcccgccggg
     1501 ccgtgcggct ccttcgcggt gctcatcttt ggctctcccc acccgcaaat cccggtatgc
     1561 cgcggcgacg ccgcccacgg accgggccgc cggcaagctt accgcatcgt gccaatcgtg
     1621 ggtaaagggc cagggcgctt ggcagggggc agccgcaggt cacggcgcca cgtcgaagag
     1681 ctcgcggaag ccgtagggtt cgtaggcgcc gatgacgagt tgctccagca cgcctttgcc
     1741 gcgctccgag ccgcgggcat ccgtgagctg cgcatcgcag attgcctgga cgtgaaaata
     1801 gccgggatct gcatcggcta tctcggcggt ctcgtagacc tcgtaattcg tcgccagctc
     1861 gccgtggaac agcccgtggc cccactccgg atgcatatag cccagcccgc gcatgtagaa
     1921 ggtctgggtc ggctgcagct caatggtcac ctcctcgccg ttttcccgca cgtactcgag
     1981 ggtggcgctc tcggccagcc gggtgccgga ccggtgccgg atgctgctcg ctgcggccgc
     2041 catctcctcg ctgcgctccg gatcggccag gcggcccatg gtgttccagg gtcgaccttc
     2101 gcgatccgcg ttgaggtggt aatagttgtt ggtgctcggg aagttcagcg gcgcccacag
     2161 ccagtagaac tggggctcac ccgcgtacgg gttgggctgg gggtcgcgtg cgcccaccga
     2221 gcggatgccc caggaccggt cccgggttcc ccaggtctcg tcgtgggtga gctcgtaacg
     2281 ctggccgtcg acctcgagcc agccctcata gctgccgttc tgggtgagcc gggtgtagtc
     2341 catgaaggtg aggctaccct gacggcgggt gaagcgcggt tcttccacgg cggggacccg
     2401 ggcacgaaaa cagacgtccg ccgccacgcc gttggcggga tccgcgcagc tcaccctcag
     2461 gacgttgagc ggctcgatga cctcgacctc gatgggaccc acctgggtgt tcagccgctc
     2521 catgtggagc actttggacg cctgcacgtt gtattgcctg ccgccgacca gaatgctgaa
     2581 ggcggcatcc atgacgttca ggtgcggata gacgccaagc gccgcagcga agaacagctc
     2641 accctcgcgc tggtacccgt tgaagaaata gcgatcgtag aagttgcgat ccgtcccgga
     2701 ataagcgatg ggctccgggg tctggtggat gggatagtcg tccgcttttg ttaacatcga
     2761 cctcttccag gggggcggaa aatcaggtta ctcgtggact gggagcgcta caagcagctg
     2821 tgcgatcagc ccaacgtctt ttcccgatgg atgctcaacc agaccatcga gctgatcgga
     2881 gagagtccgg tcgccgatac cctggcaggg gtgttgggct cgtttcccct ggagaagccg
     2941 gacgatcacc gcggcggacc cgcgacggac atgttcgttc tggaactcga atcaggtgtc
     3001 cggcgtgccg tggtggagct ggtcctcctg gctcgggaag cgggcgccgc aaccgaagcg
     3061 acctcgcatc gcgggttggg cggtttcgtc gaggcctgga gggagtatct gaactggacg
     3121 cggggctgac cgccagttgt gctgcctcgc gcccgacggt gtgccgctcc ggcgagatcg
     3181 gtaaaagaga taaagaggga gaacgacgtg agtgatgaca aggcagtagt gctcgagctg
     3241 atcgatggtt tcaacagcat tgatctggac cgcatcgtgg cgtgcttcac cgaagatgcg
     3301 gtatacgaca acattcccat gcaggccgtg cagggcaccg aggcaattcg ggcgtcgctg
     3361 gaggggttca tgggcgcggc cagcgaggtt cagtgggacc tgctcaacgt ggtcggtgag
     3421 ggtggcgtgg tgctcaccga gcggatcgac aaattcaagg tcaacggcac ctggatcgag
     3481 ctcccggtga tgggcacctt cgaagtcgcc gacggcaaga tcaaagcctg gcgcgactac
     3541 ttcgatctgg ctcagttcca gacccagttc gccaaggcga cgggcgctca gtagccggac
     3601 gagccgcgcc gtgttccggc gattgctgat tgccaaccgg ggggccatcg cccggcgggt
     3661 ggtgcgtgcc tgcaacgatc tggggatcga gagcgttgcc gtttactccg aagcggacgc
     3721 ccatgcgccg tacctgtccg aggcttctcg ggcggtgggc ctgccagggg tgagcgccgg
     3781 ggaaacgtac ctcaacacgg ccgccctcct ggaagccgtg cgcgcaagcg gcgcggacgc
     3841 cgtgcatccc gggtacggct ttctggcgga gaatgctgac tttgcccgcg cggtggcggc
     3901 tgcgggagcc acgttcgtcg ggccggatcc taagtggctc gaggtgatgg gagacaaggt
     3961 ggcggcccgt cgcctgctcg ccgaccacgg ttttcccgtc ttcccgggca gtggcggtct
     4021 cagcagtctc gacgaggctc tcagcgcagc cgccgagctg tcataccccg tgctgctcaa
     4081 accggccgcc ggaggcggcg gcatcggcat gcggctggtt cgcgacgagg cggagctggc
     4141 tagggctttc gcatctgcac aacggctggc cgagcaggcg ttcgggcagg gaggcatcta
     4201 cctcgagcgg gccatcgaca agccgcgtca cgtggagttt cagattctcg ccgatggtgc
     4261 tggtggggcg ttgcacctct acgaacgcga atgttccatt caacggcgac accagaagat
     4321 catcgaggaa gcgcccgccg tcggcctgcc gcgcgaggag ctggatgcgc tcgccgcgcg
     4381 cgcggaagcg gtgatggcag agatcggcta cgacaacgtc gggaccctgg agacgctgtg
     4441 cacgcagcag ggcgagttcg gcttcctcga gatgaacgca cgcatccagg tggagcatgc
     4501 ggtgaccgag gcggttacgg ggctcgatct ggtggggctg cagatccgcc tggccgccgg
     4561 cgcagcgctt cccgagcgcc ccgggctctg cggccatgcc gtcgaagcga gaatttacgc
     4621 ggaagatccg cgcacgctcc tgccggacac gggcgttctg cgcgttttcc gggcgccgcg
     4681 gatgcaccgg gtacgggtgg agacgggtta ccaggagggt caggccgtta cgccgttcta
     4741 cgatgccatg ctggcaaagg tcatcggctg gggcgagacc cgcgagcttg ccatcggccg
     4801 aacgctggtt gggctcaagg gattcgagat ccggggggt
//
LOCUS       DBNHKV010000448         6469 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1552023, whole genome shotgun sequence.
ACCESSION   DBNHKV010000448 DBNHKV010000000
VERSION     DBNHKV010000448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6469)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1552023"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="AC16PP_14670"
     CDS             complement(<1..257)
                     /locus_tag="AC16PP_14670"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR6482921.1"
                     /translation="MEVLQLLISGVSQGCVYGLIALGFVLIYKATEMVNFAQGDVMML
                     GAFLAFALINLMELPFLWGFMGALAAMAVVGVLLERLLLRP"
     gene            419..1105
                     /locus_tag="AC16PP_14675"
     CDS             419..1105
                     /locus_tag="AC16PP_14675"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6482922.1"
                     /translation="MTGRRLTGKRVLITQADDYMGPATAELFASHGAQIIADARDLRP
                     AGACEALVHEAGRVDVLIANLAGPNFSGTPATAIDDEAWATVFDTMVHPLHRLTRAVL
                     PQMQDRQSGKIIVYGSATALRGLKTVAAYSAARAAQVGYVQAVGVEVAPLNVQVNLIA
                     QNYVENPVYYPPELTAKESFQRSLKRQVPLGRLATAREDAQFALFLASDESDFFVGQA
                     IPFSGGWVQR"
     gene            1150..2541
                     /locus_tag="AC16PP_14680"
     CDS             1150..2541
                     /locus_tag="AC16PP_14680"
                     /inference="COORDINATES: protein motif:HMM:NF023222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6482923.1"
                     /translation="MMTGDEYRASLNDGRETFFEGKQVDDLPNHPILGITVESAASGY
                     DRFYDPAPDAVGAFMKVPGSAVELREQVELHETVDLLTHVTYASIMTLLTAADRIEAQ
                     LPDNARRIREYVRDVQRKDLRITQCITDAKGDRSRPPGKQDDPDAYVRVIERRADGVV
                     IRGAKLHISAASMGHELMTIPTKSMKPGEDDYAVACMVPVNAPGVKIVNTTYAPRHED
                     KRDFPISGSHHTPEGFVIFDDVFVPDERVFLDGPPEFAAVFAHSLGLWERLGGLASMA
                     SSYDRLVGFAQLICEANGLERTGHIKEKISEMMINATLIRASLEAAIDNCSITSDGAA
                     FPNELYTNVGKYHGAANWSAMVRHLHDIAGGSILTAPGMADLENEKTGHLIRKYMGTT
                     QEVDGVYRTRLFHAIRDMTADALGGWNAVTNIQAGGGLYAQRIVSRMHYDIDKAKRMA
                     LESAHMQEYLSDA"
     gene            2603..2803
                     /locus_tag="AC16PP_14685"
     CDS             2603..2803
                     /locus_tag="AC16PP_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482924.1"
                     /translation="MKNFRIRPLHRQRNGRRQRVRVLDQNELADLLGVPITELRKELD
                     DRGWSYHEDSTGNLWATRPERP"
     gene            3213..3632
                     /locus_tag="AC16PP_14690"
     CDS             3213..3632
                     /locus_tag="AC16PP_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6482925.1"
                     /translation="MKYLHTMVRVTDLDDSLDFYCAKLGLHEVGRHESEAGRFTLVFL
                     AAPGDEEAQVELTYNWDPQEYTGGRNFGHLAYQVEDIYALCQHLMDSGVTINRPPRDG
                     NMAFIRSPDGISVELLQKGGALAPADPWKDMPNVGEW"
     gene            3626..5842
                     /locus_tag="AC16PP_14695"
     CDS             3626..5842
                     /locus_tag="AC16PP_14695"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR6482926.1"
                     /translation="MVAWRLGPAAFAALVWGLTGATSSSLAAAADQAAGAQTLAPPRV
                     VLVLSGGGARAAGHIGVLKALEELRVPVHGIVGTSMGAVVGGLYAAGHSPAGMERLVA
                     RVDWQELLIDDPPRESFRFRRKREDASLFVQAASGVDTEGLKLPKGVVHGHRLKAFLA
                     RHTLHVGDVDDFRQLSIPFASIATDISTGEAVELTGGNLAEAMFASLAIPAFVGPTEI
                     DGRLLVDGGVSNNLPVDVALAMGADVIIAVDLSSPLRGRDELGSVLEVTDQLTSILTR
                     RNTTEQIARLRPQDVLIVPELGGVTAIDFDLAATTIPSGYSAAIAARPRLASLALPEE
                     PFEQHVAGLRRQEVTTIDVAEVVLTTDSRVDADLLLARTGLQPGTMEVAQIEAAVDEL
                     YGMELFSQVPYAYQHGELHINPIRRDWGPNYLQFGLALEDNLEGRNHYNLGVAFTATE
                     LNAKAGEWRTELGIGERPRLVTEFFQPFGPRGRWYVNPSVGVSAFNRGVFETDNLVAE
                     YQIRQVAGTLATGYAFGSRAEARIGLSLAEGTRRRLVGDPTFEKTSADSGMAFVEFRY
                     DSLDDLYFPQRGSTLQARWTLSEESLGAEVDYRAAELAASRALSFGRHTLVVGGEFAT
                     VYEGEAPFHEQYNLGGFLNLSGLEVDQKLGQHRGIGRVVYYYRWSSNPVLPVYLGMSA
                     EAGQVWIEDDEIGFGNLEAAGSLFLGLDSPIGPVFLAGGMAEGGNKSIYVFLGQPF"
     gene            6046..>6469
                     /locus_tag="AC16PP_14700"
     CDS             6046..>6469
                     /locus_tag="AC16PP_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482927.1"
                     /translation="MRTTYLTAGAIALAIVVWLLSGQMASGDPVRHQTLAENNAEALA
                     RTQDKAPTRVRARVMNATPQFQHVVLRGKTESKRVVEVRSETSGRVIERPVERGAYVT
                     QGDLLCRLAMDDREAALAEARESLNQARIEYEGSLKLKQ"
BASE COUNT         1196 a         2168 c         2039 g         1066 t
ORIGIN      
        1 atcggacgca gcagcagccg ctcgagcagc acccccacca cggccatggc cgcgagcgcg
       61 cccatgaagc cccaaagaaa gggaagctcc atgaggttga tcagggcgaa ggccaggaac
      121 gcgccgagca tcatgacgtc gccctgggcg aaattgacca tctccgtggc cttgtagatc
      181 agcacgaacc ccagggcgat caatccatag acgcacccct gcgacactcc gctgattagc
      241 aactgcagaa cttccacgtc ttggctggcg cgttttggtt tttgatcccc tcggacgcct
      301 attgtccgaa tcgacggctg ctttgtcatc tcggatccgt gccagcgcca ttcccgccag
      361 gacccgctcc gggcagaatg gacggcacac ccggagatca ggcagcggag cgaggaaaat
      421 gaccggacgg cgactcacgg gcaaacgggt gctcatcacc caggccgacg actacatggg
      481 gccagccacg gccgagctgt tcgccagcca cggcgcgcag atcatcgccg acgcccggga
      541 cctcagaccg gcgggcgcgt gcgaggccct tgtccacgag gccggccggg tcgacgtgct
      601 gatcgccaac ctggccgggc ccaacttcag cggcacgccc gccacggcca tcgacgacga
      661 ggcctgggca accgtcttcg acaccatggt tcaccccctg catcgcctga cccgcgcggt
      721 tctgccgcag atgcaggacc gccaatccgg caagatcatc gtctacggga gcgccacggc
      781 gctgcggggg ctgaagaccg tcgccgccta cagcgccgcc cgcgccgccc aggtgggcta
      841 cgtgcaggcg gtgggcgtgg aggttgcgcc gctcaacgtc caggtcaacc tcatcgccca
      901 gaactacgtg gagaaccccg tctactaccc gccggagctg acggccaagg agtcgttcca
      961 gcgatcactc aagcgccagg tgccgctcgg caggctggcc accgcccggg aggatgcgca
     1021 gttcgcgctg ttcctggcca gcgacgagag cgacttcttc gtcggccagg ccattccctt
     1081 cagcggcggt tgggttcaac gctaggctca tttaccatcc ttcgcgttgg gcttgagggg
     1141 gattgccaga tgatgaccgg cgacgagtat cgcgcctcac tgaacgacgg gcgcgagacg
     1201 ttcttcgagg gtaagcaggt ggacgacctg cccaaccacc cgatcctcgg gatcaccgtg
     1261 gagtcagcgg ccagcggcta cgaccgcttc tacgatcccg ccccggacgc ggttggcgcc
     1321 ttcatgaagg tgcccggctc cgcggtcgag ctgcgcgagc aggtggagct ccacgagacc
     1381 gtggatctgc tgacccacgt cacctacgcc tccatcatga cgctgctgac cgcggcggac
     1441 cggatcgaag cgcagctgcc cgataacgcg cggcgcatcc gcgagtacgt ccgcgacgtt
     1501 cagcgcaaag acctgcgcat cacccagtgc atcaccgacg ccaagggcga ccgcagccgg
     1561 ccaccgggca agcaggacga ccccgacgcc tacgtgcgcg tcatcgagcg acgggccgac
     1621 ggcgtggtca tccggggggc caagctgcac atctcggcag cctccatggg ccacgagctg
     1681 atgaccatcc cgaccaagtc catgaagccc ggcgaggacg actacgccgt ggcatgcatg
     1741 gttcccgtca acgcgccggg ggttaagatc gtgaacacca cctatgcccc acgacacgag
     1801 gacaagcgcg actttccgat ctccggatca caccacacgc ccgagggctt cgtgatcttc
     1861 gacgacgtgt tcgtgcccga cgagcgggtg ttcctggacg gcccccccga gttcgccgcc
     1921 gtgttcgccc actccctggg cctgtgggaa cggctcggcg gcctggcgtc tatggcgtcg
     1981 agctacgacc ggctggtggg atttgcgcag ctcatctgcg aggcaaacgg gctggagcgc
     2041 acggggcaca tcaaggagaa gatctccgag atgatgatca acgccaccct catccgcgcg
     2101 agcctcgagg ccgccatcga caactgcagc atcacctcgg acggcgcggc gttcccgaac
     2161 gagctttaca ccaacgtggg aaagtatcac ggggccgcca actggtccgc catggtccgt
     2221 catctccacg acatcgccgg tggcagcatc ctcaccgcgc cgggcatggc cgacctggag
     2281 aacgagaaga cgggacatct gatccgcaag tacatgggca cgacgcagga ggtggacggc
     2341 gtttaccgca cccggctctt ccacgccatt cgcgacatga ccgccgacgc gctgggcggg
     2401 tggaacgccg tgaccaacat ccaggccggc ggcggcctct acgcgcaacg gatcgtgtcg
     2461 cgcatgcact acgacatcga caaggccaag cggatggcgc tggagtccgc ccacatgcag
     2521 gagtatctca gcgacgcctg atcgttgcgc cgcatactgt atgtatatac agttattggg
     2581 accgagccgc gcagcacagc ggatgaagaa tttcaggatt cggccgctgc accgtcagcg
     2641 caacggccgc cggcaaagag tgcgggtgct cgaccagaac gagctggccg acctgctcgg
     2701 ggtgccgatt accgaattgc gcaaggaact ggacgatcgg ggttggtcct accatgagga
     2761 cagcaccggc aacctgtggg cgacccgccc agaacgccca tgagacgggc gtttccgccc
     2821 ttttgaaccc tccatattca ttccagaatc cttatgtcct ggctgcagag ttaccgccta
     2881 acctctatcg tgttgttttg aacgggccgg agcggatgcg agatctgcgt aacgaacccc
     2941 atacgcaaat gtccagtgcg gcagcgatag ccgcacacag ggaccagggg cagctgaccc
     3001 taggtgagag cgcactactc ctcggcgctc tgctcaccct gatgctgagc ctggctgcat
     3061 tcgtaaccct ctagctgaag agcaacacac tctccataga ccttgtgatg accaaatggc
     3121 tagccccgct tcggcggggc ttttttttgc cctgaagaaa ccgggcggta tgatgcacgc
     3181 gccccggctg atccgggcga ggaaaacatt ccatgaaata tctgcacaca atggtccggg
     3241 tgactgacct ggacgactca ctcgactttt actgcgcgaa gctcggcctt cacgaggtcg
     3301 gtcggcacga aagcgaggcg gggcgtttca cgctcgtctt cctggccgct ccaggcgacg
     3361 aggaagcaca ggtggagctg acctacaact gggacccgca ggagtacacg ggcggccgta
     3421 acttcgggca cctcgcctac caggtcgaag acatctacgc gctgtgccag cacctgatgg
     3481 actcgggcgt caccatcaac cgcccgccac gtgacgggaa catggctttc atccggtcac
     3541 ccgatggcat ctcggtagag ctgctgcaga agggcggggc tctggcgccc gccgatccct
     3601 ggaaggacat gccgaacgtc ggcgaatggt agcgtggcgc ctcgggcccg cggcctttgc
     3661 tgcgctggtc tggggcctca cgggcgcaac ctcatcatcc ctggctgctg ccgccgacca
     3721 ggccgcagga gcgcagacgc tcgccccacc tagggtggtg ctggtgctca gcggcggcgg
     3781 cgcccgggcc gccgggcaca tcggcgtgct caaagcgctc gaggagttgc gggtccccgt
     3841 tcacggcatc gtcggcacca gcatgggcgc cgtggtcggc ggcctctacg cggccggtca
     3901 ctcgccggcg ggaatggaac ggctcgttgc ccgtgtggat tggcaggaac tgctcatcga
     3961 cgatccgccc cgggaatcct tccgtttccg acgcaagcgc gaagatgcga gtcttttcgt
     4021 ccaggccgcg tccggcgtcg atacggaagg tctgaagctt cccaagggcg tggttcacgg
     4081 ccaccgcctg aaggcgtttc tcgcccggca caccctgcac gtcggggacg tcgacgactt
     4141 ccgccagctg agcataccgt tcgccagcat cgccacggac atcagcaccg gggaggccgt
     4201 ggagctcacc gggggcaatc tcgccgaagc gatgttcgcc agcctcgcca tacccgcttt
     4261 cgtcggcccc acggagatcg atgggcgtct cctcgtggac ggcggcgttt ccaacaacct
     4321 gccggtggac gtggccctgg ccatgggtgc cgacgtgatc atcgccgtgg atctgagctc
     4381 tcccctgcgc ggccgcgacg agctcggctc cgtcctggag gtgaccgacc agctgaccag
     4441 cattctcacc cgccgcaaca ccacggagca gattgcccgg ttgcgcccac aggacgtgct
     4501 catcgtgccc gagctcggcg gggtgaccgc catcgatttc gacctggcag caaccaccat
     4561 cccgtccggc tacagcgcgg ccatcgccgc ccggcctcgc ctggcatcac tggcgttgcc
     4621 ggaagagccc ttcgagcagc acgtcgccgg tctgcgccgg caagaggtca ccaccataga
     4681 cgtcgcggag gtggtgctga ccaccgacag ccgggtggac gccgatcttc tgctggcccg
     4741 gacgggactg cagcccggca ccatggaggt cgcgcagatc gaagccgccg tggacgagct
     4801 ctacggcatg gagcttttct cccaggtgcc ctacgcgtat cagcacgggg agctccacat
     4861 caaccccatc aggcgggact ggggtccgaa ctatctgcag ttcggactcg cgctcgagga
     4921 caacctggaa ggacgcaatc attacaatct gggcgtcgcc ttcaccgcca cggagctcaa
     4981 cgcaaaggcc ggcgagtggc gaacggagct cggaatcggc gagcgtccgc gcctggttac
     5041 cgagttcttc cagcccttcg gcccccgggg acgctggtac gtgaacccct cagtgggcgt
     5101 aagcgccttc aatcgcggcg tgttcgagac ggacaacctg gtcgccgagt accagatccg
     5161 gcaggtcgca ggcaccctgg ccacggggta cgcattcggc agccgggccg aggcccgcat
     5221 cgggctcagc ctggccgagg gtacgcggcg gcgcctcgtc ggcgacccta ccttcgagaa
     5281 gacgtccgcg gactcgggaa tggccttcgt cgagtttcgc tacgacagcc tggatgatct
     5341 gtactttccg cagcgtggaa gcaccctgca ggccaggtgg acgctttcgg aggaatctct
     5401 gggggcggag gtcgactacc gtgccgcgga gcttgccgct tctcgagcgc tgagcttcgg
     5461 gcgccacacc ctggtggtcg gcggggaatt cgccacggtc tacgaaggcg aagccccctt
     5521 tcacgagcag tacaatctgg gcggattcct caatctctcc ggcctggagg tcgaccagaa
     5581 gctcggtcag caccggggca ttggaagggt cgtgtactac taccgctggt cttcgaaccc
     5641 cgttctgccc gtctatcttg gcatgtcggc agaagccggc caggtctgga tcgaggacga
     5701 cgagatcggc ttcggcaacc tggaagcggc cggcagcctg tttcttggtc tggacagtcc
     5761 catagggccc gtttttctcg ccgggggcat ggccgaaggg gggaacaagt ccatctacgt
     5821 cttcctcggg caaccgtttt agccgcaagg ccgcctttag agccacatcg cgcggcgttt
     5881 tcacgccgcc tacacaccgc catcgctacc tttaaccctg acagcttcca ggcccgccga
     5941 gcatccttga ttgtgcagcg gcccatggaa ataccaccgg cagtttgtta ctttaggtaa
     6001 cgtattgggt gctttagtaa caccacttgc tcatcggaga ccaaagtgcg aaccacatat
     6061 ctcaccgcag gcgccatcgc gctggccatc gttgtctggc tgctttcggg ccagatggcg
     6121 agcggcgacc ctgtcaggca tcagacgctg gcagagaaca acgcggaagc cctcgcgcgg
     6181 acccaggaca aggcaccgac cagggtgcgc gcccgggtaa tgaacgccac gccgcagttt
     6241 cagcacgtcg tcttgagggg gaaaaccgaa tcaaagcgcg tcgtcgaggt ccgctccgag
     6301 acgtccggcc gcgtgatcga gagaccagtc gagcgcggtg cgtacgttac ccagggggat
     6361 ctcctgtgcc ggcttgccat ggacgatcgc gaggctgcac tggccgaggc ccgcgaatcc
     6421 ctcaatcagg cccggatcga gtatgaaggg agcctgaagc tcaagcagc
//
LOCUS       DBNHKV010000449        21974 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_61475, whole genome shotgun sequence.
ACCESSION   DBNHKV010000449 DBNHKV010000000
VERSION     DBNHKV010000449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 21974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21974
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_61475"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="AC16PP_14705"
     CDS             complement(<1..1031)
                     /locus_tag="AC16PP_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HIR6482928.1"
                     /translation="MHFSVILRVTGTLLMLFSIALLLPVVVAVYYGESTTRTFALAFA
                     ITLISGLALTLVARSRRELRSGDGFLITVLFYLGLGLFGAIPIYLAESVDASFTDAAF
                     ESLSGLTTTGATVLTGLDELPRSILFYRQLLQWLGGMGIIVLAVAILPMLGIGGMQLY
                     RAESPGPVKDAKLTPRITETAKALWYLYVGITASCALAYYAAGMDAFDALCHSFSTVA
                     IGGFSTHDASIGHFDSAAVEGVAIFFMILAGINFGLHFMAWNRRNPFLYTRDAEVRLY
                     LIVLVGVAALVCAILASYPDATESPWRDGLFQAVSIATTTGFTTQDFSVWPSAAPVLL
                     MFAAFAGGCA"
     gene            complement(1031..2404)
                     /gene="trkA"
                     /locus_tag="AC16PP_14710"
     CDS             complement(1031..2404)
                     /gene="trkA"
                     /locus_tag="AC16PP_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016489939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HIR6482929.1"
                     /translation="MKILILGAGQVGGTLAENLAKESIDITLVDNNIEALEELQTKLD
                     IKTVFGSASHPDVLRKAGADDADMLIAVTSSDEVNMVACQVCYSLFRTPQKIARVRSS
                     AYLKGEGFFSGEHMPIDVLINPEEVVTENIRQLLEHPGALQVLGFADDRVQLVAVKAY
                     YGGPLVGQELRFLRQHMPNIDTRVAAIFRRGRAITPEGNTVIEADDEVFFIAAAEHIN
                     AVMSELRRVERPFKRVMIAGGGNIGVKLAQSIEPYFSVKVLEQNARRCEELAEVLDRS
                     VVLNIDATDRELLLEENIEQCDAFCAVTNDDEVNIMSSLLAKRLGVRQVITLIGKPAY
                     VDLMQGGDIDIAISPQQATTSELLTHVRRADVVKVHSLRRGAAEAIEAIAHGDAKNSK
                     VVGRAIGDVDLPTGASIGAVVRGKEVFVGRDDVVVEPDDHVILFLADKRQVAAVERLF
                     QVGLTFF"
     gene            complement(2418..3686)
                     /gene="rsmB"
                     /locus_tag="AC16PP_14715"
     CDS             complement(2418..3686)
                     /gene="rsmB"
                     /locus_tag="AC16PP_14715"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF008149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR6482930.1"
                     /translation="MPSPAEGAPATASRLLLAVLRDKQTSDQALERIPSTPQVRDTFY
                     GTLRHVFCLQRGVDKALRKPLANKHRDVYCLMLVGAYQLAYARVPDHAAINETVAGCR
                     ALRKPWATGLVNAVLRHIQRKGLPNDCSDASEHPAWMAERIRRDFPDRAGAIFCANMG
                     RAPMCVRINRRLVATDGYRQRLADSGIAFSEGFQDEFIILTAAMPTARLPGFDDGLAA
                     IQDAGAGFAADILAPEPGSRVLDACAAPGGKLFHLVERFPDAATLAIDTSTARLERLE
                     AEAARLHHNVNAQQGDATDLRWWDGQPFDRVLLDAPCTGSGTLRRHPDIRLLRAASDL
                     DDYARLQGRLLTNLWQTLRPGGNLLYCTCSLFAEENDEVIGAFLKAADDAEARPLSLA
                     TGVARAHGWQLLPTDSRTDGFYYALLTKRV"
     gene            complement(3670..4635)
                     /gene="fmt"
                     /locus_tag="AC16PP_14720"
     CDS             complement(3670..4635)
                     /gene="fmt"
                     /locus_tag="AC16PP_14720"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015474830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR6482931.1"
                     /translation="MTRAGGSAPRLGFAGTPEFAAAILQALVTAGHSPVVVYTQPDRR
                     SGRGRKALPSPVKALAAKQGIETRQPATLRETGAAADLAGLELDTLVVAAYGLILPQA
                     ILSAPRYGCMNVHASLLPRWRGAAPVERAIMAGDEETGVTIMQMDAGLDTGPIIEQER
                     CPIESATTGGELEATLARLGARCLLDCLGNLGSLDPTPQDEAGATYAHKLTPADASID
                     WNRPAEAIERQIRALNPRLPATCASGDLRIRILQARAIRDPGGEPGRIVSADRNGIRV
                     SCRESTLVIQSVQLDRGRGRPLSAADAVNGYAAYFEEGTKLAQPS"
     gene            complement(4632..5135)
                     /gene="def"
                     /locus_tag="AC16PP_14725"
     CDS             complement(4632..5135)
                     /gene="def"
                     /locus_tag="AC16PP_14725"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010430165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR6482932.1"
                     /translation="MATLDILEYPDPRLRTQAKPVTEVDAQVQQLVEDMFETMYAAPG
                     IGLAASQVNVHQRIIVIDVSENRDEPHVFINPELSLQGAAIESEEGCLSVPGFYEPVI
                     RSEQVIVSALDRDGQSFTLEAQGMLAVCIQHECDHLEGKLFVDYLSNLKRTRIRKKLD
                     KQRRVRA"
     gene            5432..6505
                     /locus_tag="AC16PP_14730"
     CDS             5432..6505
                     /locus_tag="AC16PP_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010796361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6482933.1"
                     /translation="MRKLRIIFQVLIGIGLGISASAWSATDGLAQYLKPGHPEVYTVV
                     KGDTLWDISGKFLDRPWLWPEIWRINPQIDNPHLIYPGDQIALEWVEGQPRLSLRRGD
                     AARTYKMTPADSVSLQPQIRSTPLESAIPTIRLEAIQGFLVQNRVVDPKVLSSAPYVV
                     QGENERLVLGAGDRLYVRGVLPDSESFNVVRKGPLYVDPETKEVLGQEATYIGLGRAV
                     AQEDDIATMSLQSTREEAQIGDRVLPTEERRVDSTFFPSAPDGDVNGQIISVFGGVTQ
                     VGQYDVVVLNRGEREGIEVGNVMAIYKRGALVKDRIANQTIRLPSERAGLLMVFRAFE
                     KLSYGLVLETERPLAVNDEVRNP"
     gene            6614..7702
                     /gene="dprA"
                     /locus_tag="AC16PP_14735"
     CDS             6614..7702
                     /gene="dprA"
                     /locus_tag="AC16PP_14735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR6482934.1"
                     /translation="MGDLAGDVESENAAAACLAMELGRPRARAAIAAHGNATSAVEAR
                     AASRDWNPRLVPQRLLADCARHGISLLGGEDACFPELLRHIPDPPLALFSLGALPGRD
                     GTAVAVVGARRCSRLGREVAYQLARQIATAGVRVVSGLALGIDKAAHEGALDAPGAGR
                     TVAVLGCGLARIYPRRNQALAERVVGEGGTLISEYPPEVPPAKHRFPERNRLISGLSH
                     GVVVVEAGDRSGSLITARLALEQGREVMAVPGPVSGGTSRGCHRLLREGAALVECAEH
                     VFEALNLTRPTPGSAETEQRAGLDLGEPLNSVLSAVEPVTTTMDQLVAVTGMAPEALS
                     VCLVDLELAGFVERVHEGYIRRPFSPGI"
     gene            7727..8227
                     /gene="purE"
                     /locus_tag="AC16PP_14740"
     CDS             7727..8227
                     /gene="purE"
                     /locus_tag="AC16PP_14740"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR6482935.1"
                     /translation="MSFVAILMGSNSDWPVMQSTTEVLGALDVEFEVRVTSAHRTPAA
                     TMEYVQDATQRGCAVFICAAGMAAHLAGAVAAHTTRPVIGVPIDSGPLSGFDALLSTV
                     QMPGGIPVATVAVGKAGAKNAGYLAAQILGLGDAALADRVASERAENARKVQSQNEQL
                     QEELGR"
     gene            8227..8784
                     /locus_tag="AC16PP_14745"
     CDS             8227..8784
                     /locus_tag="AC16PP_14745"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR6482936.1"
                     /translation="MTSVHVARAVRGLKAGGVVLHATEGVWGLACDPFDVEAVARVLV
                     LKGRPVAKGLILVGGNMDHFAEELDLLEPAVRSQVESTWPGAVTWVLPSTRFPEWISG
                     GRETVAVRVSGHPQVRALCERFGGALVSTSANPTGRLPATSQIRARAYFQGRVDYVLP
                     GEVLNAGAPSRIQTLGGEVLRGGGP"
     gene            8781..9644
                     /gene="aroE"
                     /locus_tag="AC16PP_14750"
     CDS             8781..9644
                     /gene="aroE"
                     /locus_tag="AC16PP_14750"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014869363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR6482937.1"
                     /translation="MSAGKPGEPDRYAVIGHPVSHSLSPAIHAMFAEQTGQHLAYAKL
                     PAPRDGFARTASDFFAGGGSGLNVTVPFKQEAAGWVDLLEGAAREAGAVNTISLESGQ
                     TKGFNTDGLGLVRDLTVNHGLSISGARVLVVGAGGATQGVVRPLIALGPQALTVANRT
                     VSKAAELVGSLGSAAGRARVAACAPEAATGPFDLVINATSAGLEGHGEVVPADAAAGA
                     VCYDMVYSTGAGAATAFCEWALGVGAVRAIDGLGMLVEQAAEAFLIWRGVRPDTAPVI
                     DALRRVAPPAY"
     gene            9665..9904
                     /locus_tag="AC16PP_14755"
     CDS             9665..9904
                     /locus_tag="AC16PP_14755"
                     /inference="COORDINATES: protein motif:HMM:NF021072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HIR6482938.1"
                     /translation="MTIKKRFIAGAVCPDCGAQDRLVVEEMPDGKRRRCVSCGFTDVS
                     ASEPEVALPTRFARRGVDSEQARPVRLLGEGDSDS"
     gene            10282..11397
                     /gene="coxB"
                     /locus_tag="AC16PP_14760"
     CDS             10282..11397
                     /gene="coxB"
                     /locus_tag="AC16PP_14760"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR6482939.1"
                     /translation="MGLGLAAAARADELNMTPGVTQISRSIYDLHMVIFWICVAIGVV
                     VFGVMFWSIFQHRKSRGAQAASFHESTRLEIAWTLIPILILIGMAFPATQTLVAMYDT
                     GGEDMAVEVRGYQWKWQYKYLDEDYNSTFSFFSNLATSRDEIENRVVKNDNYLLEVDE
                     PLRIPANRKVRFLITSEDVIHAWWVPDFGIKRDAIPGMLNELWTVVETPGVYRGQCTE
                     LCGMDHGFMPIVVEVMAEEDYDAWYADRVRMEEARHEALSKTFTPAELMEEGERVYAT
                     FCASCHQANGKGLPPVFPALAGSPIATGPLSDHLDIIVNGRAGTSMNAFGNQLDAAEI
                     AAVTHYERNAWGNDVGDVTQPIDVIEFMSGKEAEETP"
     gene            11394..12995
                     /gene="ctaD"
                     /locus_tag="AC16PP_14765"
     CDS             11394..12995
                     /gene="ctaD"
                     /locus_tag="AC16PP_14765"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR6482940.1"
                     /translation="MSEAVLTHHDDHHHGPASGITRWLFTTNHKDIGTLYLWFSFLML
                     LIGGAMALVIRTELFQPGLQFVEPAFFNQMTTNHGLIMVFGAIMPAFVGLANWLLPMM
                     VGAPDMALPRMNNLSFWILPFAFGMLISTLFMEGGGPNFGWTFYAPLSTTYAPDTVTY
                     FIFGVHLMGASSIMGSINIIATILNMRAPGMTLMKMPLFVWTWLITAYLLLAVMPVLA
                     GAVTMMLFDIHFGTSFFNAAGGGDPVLFQHIFWFFGHPEVYIMILPAFGVVSQIIPTF
                     ARKPLFGYDSMVYATASIAFLSFIVWAHHMFTVGMPLAGQLFFMYATMLIAVPTGVKV
                     FNWVSTMWRGSMTFETPMLFAIAFVVLFTIGGFSGLMLAIAPADYQYHDTYFVVAHFH
                     YVLVPGALFSIIAAVYYWLPKWCGRMYDETLGKVHFWLSFIGVNVTFFPQHFSGLAGM
                     PRRIPDYALQFADFNMVSSVGAFIFGFSQLVFLYVVIKAIRSGRPATDRVWEGAHGLE
                     WTLPSPAPYHSFTTPPTVTEAEAHS"
     gene            13035..13571
                     /locus_tag="AC16PP_14770"
     CDS             13035..13571
                     /locus_tag="AC16PP_14770"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HIR6482941.1"
                     /translation="MHKSSKGIVWKLGLMVVGMFGFGFALVPLYDLLCEVTGLGGRTG
                     GPYAYDPAVVEVDRSRLVKVNLLTNTNDGMPWRFWSEVGGMRVHPGEAKDVKFYVKNT
                     TDRRMVGQAVPSVVPITATDYFHKTECFCFESQVLEPGEEMELPLRFIVDAGLPKNVQ
                     TISLSYALFDATGFAAAE"
     gene            13600..14514
                     /locus_tag="AC16PP_14775"
     CDS             13600..14514
                     /locus_tag="AC16PP_14775"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR6482942.1"
                     /translation="MASSSDQATYYIPHSGWYPIWVAVGSALLLFGLGSWLNDLKAET
                     EPSRALFYTGAVILSVVLFAWFAKVVNENLEGLTSPQLHRSYIWGISWFIFSEVMFFF
                     AFFGALLYARVLSVQWLGGEGAKGFTGEYLWPEFEAVWPVISNPDPAVFENPRGDMAS
                     PGLLSWGGYLPFWNTVILLTSSVTVHFAHTAIKNADRTRLLRWLGLTVALGVVFLFLQ
                     VEEYVHAYQELGLTLSSGIYGSTFFLLTGFHGFHVTLGTFMLFVQLVRAFRGHFQPKD
                     CFGFEASSWYWHFVDVVWVCLFLFVYVF"
     gene            complement(14521..14748)
                     /locus_tag="AC16PP_14780"
     CDS             complement(14521..14748)
                     /locus_tag="AC16PP_14780"
                     /inference="COORDINATES: protein motif:HMM:NF022587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2909 domain-containing protein"
                     /protein_id="HIR6482943.1"
                     /translation="MIIFLLAGVVLSLFSSLAFLFRDSERPESKRALYALGIRITLAG
                     ALLLTVLYGFATGELSMGSNAPWHQSQTSGD"
     gene            14872..15564
                     /locus_tag="AC16PP_14785"
     CDS             14872..15564
                     /locus_tag="AC16PP_14785"
                     /inference="COORDINATES: protein motif:HMM:NF014193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial respiratory chain
                     complex IV assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HIR6482944.1"
                     /translation="MTLLVPLMLGLGFWQLDRADQKRAHQDTLFSRLASAPVAGRDTL
                     APEPFQRVRLEGRFEADRYFLVDNQVADGVVGYWVVQSFSTPQGVRWLVNRGWIAGGD
                     RRDQLPRVSAPTGTVVTVGVAWPDLGLVPLLDEDDWPLAWPVRVQRLDVARMAERLED
                     AVPVEIRLETAQPGALRAAPLTVGLQAERHEGYAAQWFALAGVLIVGYAVYGLRGAQR
                     ARRLNGRTDESR"
     gene            15561..16127
                     /locus_tag="AC16PP_14790"
     CDS             15561..16127
                     /locus_tag="AC16PP_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482945.1"
                     /translation="MTQAEDKTGDQRGRAGRSQLVVILLVAGFSMAGAYLVFFLASSG
                     GVWGTTNNGEFVTPAMTMEALDLRDAAGTSATADGHWWLWTVAPGGCGEQCRFAVERL
                     RQVHALLNKDASRVRRALVTSTPGLEPPPGLDVSDLERLVGPADGLASGIYIVDPIGN
                     LVLRYPLSDAGEAVLEDLKRLLKVSQIG"
     gene            16171..17103
                     /locus_tag="AC16PP_14795"
     CDS             16171..17103
                     /locus_tag="AC16PP_14795"
                     /EC_number="1.17.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR6482946.1"
                     /translation="MLVVVLGAYTRLADAGLGCPDWPGCYGFLTVPESAEDIARAETL
                     FPDAPVERAKAWWEMVHRYFAAGLGLLVLVMAGRAYLRREQGGPVKLPLALLALVILQ
                     GAFGAWTVTMKLWPQVVTAHLLGGFTTLSLLWLLCLRLGVGSGWEVSGKLRTPALIAL
                     IVLVMQIALGGWVTSNYAALACPDFPTCHGEWLPEMDIGEGFNFAQSVGPNYLGGMLE
                     SEARVAIQLGHRLGALVATLAVGWLGLLLLRTGSPLGWPVLVLLAVQVVLGILNVLLA
                     LPLSNATLHNGFGAVLLLALVTVNYRAYLGGMRS"
     gene            17100..18011
                     /gene="cyoE"
                     /locus_tag="AC16PP_14800"
     CDS             17100..18011
                     /gene="cyoE"
                     /locus_tag="AC16PP_14800"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016782738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR6482947.1"
                     /translation="MSEVSLQAASWSDYLEMTKPRVVLLMLLCALVGMFLAVPGMVPV
                     DVLVFGLVGIALVAGSAAVVNHIADAQIDARMARTQARPVATGRVGLLQGLLFSAVLG
                     VSGMVVLYFLVNPLTAWLNLASWIGYGLIYTLYLKRATPQNIVIGGLFGAAPPLFGWA
                     AVSNSVEPGALLLVLIIFAWTPPHFWALALDRKEEYASVDVPMLPVTHGERYTRLHIL
                     YYTLILVAVSLLPFVIGMSGWLYLAGATALGGGFIYWAVVLLRNRNPRAPLETFRYSI
                     VYLGLLFVALLADHYVFADHLVGSSLQ"
     gene            18008..18655
                     /locus_tag="AC16PP_14805"
     CDS             18008..18655
                     /locus_tag="AC16PP_14805"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR6482948.1"
                     /translation="MRSVTATVLLCLSLAAAAVGAFVYSVLREPVLSDAELRDLGVFV
                     LPRPRAIGAFQLESTRGGSFTAADLQGGWSFLFFGFTNCPDVCPTTMAVLAQAERELL
                     AGSPEDADRFAGVLVSVDPERDDVRTLGEYTAAFSPRFFGVTGPFDELASFARQLNVG
                     FGKVPAPEGSFSEYLVDHGAQIVIINPQGHYHGFIRAPHTADTIVQTFASLAARF"
     gene            complement(18791..19756)
                     /locus_tag="AC16PP_14810"
     CDS             complement(18791..19756)
                     /locus_tag="AC16PP_14810"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR6482949.1"
                     /translation="MNAITEPGHPGIEETLTRYGVGELISAWPGSAASRCAYRVRTRR
                     AGREHDYLLLRVQAPRFEAELAALEACDRAGLPVAKAIPNLDGQPVDTLNGETVILTR
                     RLPGRHVYNPTVRQVAALGRFMARFHEAGGNLGPLPAWAWDQRWLAEASAALDGRLSY
                     PSARLMEATVQQVVSMLDREDVAGLPAGAIHGDICRDNVLFNEHGLSGVGGFSQVSSG
                     FLVYDLAVAANDWCIDANGMLDPERTLGFLRAYQKLRPLVRAELWFFSGFTLYAALNG
                     WIKRLAFAQGRQVSPGLHFNTPLELEKVVEQHAAHFFYLDERLLT"
     gene            complement(19787..20047)
                     /locus_tag="AC16PP_14815"
     CDS             complement(19787..20047)
                     /locus_tag="AC16PP_14815"
                     /inference="COORDINATES: protein motif:HMM:NF022639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HIR6482950.1"
                     /translation="MAAAATLPPTALASEAQDGLQGPDITIIAQEDKTILEFRQAGQL
                     RMVKIVPRLGKPYYLVPRDSTTGFGDLERADMLLPSWVIVEF"
     gene            20172..>21974
                     /locus_tag="AC16PP_14820"
     CDS             20172..>21974
                     /locus_tag="AC16PP_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004578937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HIR6482951.1"
                     /translation="MASDETAGAVSPPLLLVDGSSYLFRAYHALPELKTSTGLHTGAV
                     RGVIGMLRRLAKDYRESPIAVVFDAKGKTFRDDIYVQYKANRPPMPDDLSEQIEPIHQ
                     IIQAMGLPLIVVPGVEADDVIGTLAAEATRKRRDTVISTSDKDMAQLVTEHVTLVNTM
                     SETTLDPEGVVDKFGVPPELIIDYLALMGDSVDNIPGVPKVGPKTAAKWLNEYGDLDG
                     VVASAEQIKGKVGESLRDHLDQLPLSKALTTIKCDVALEVGIDDLAPRPVDEDRLKGL
                     FERLEFRSWLAEVGGPQEDAPAPAAASAEAEGEYECVSSAEALDEWIERLRAAPLFSF
                     DTETTSLNYMLAELVGVSFSCEPGKAAYVPLAHDYPGAPDQLGVAAALDRLKPLLEDP
                     AVIKVGQNLKYDMSVLQRYDVQLAGVGFDTMLESYVLNSTASRHNMDDLAKNYLSRET
                     IHYEDIAGKGAKQLTFNQVPVEQAVSYAGEDADITLQLHQTLWPRLEKEPRLTEVFRE
                     IEMPLLSVLSRMERKGTLVDGALLEAHGRELAERMRGITEEAWTLAGEEFNLDSPKQL
                     QAILFDKLELPVLKKTPKGQPSTAEPVLVDVARDF"
BASE COUNT         3617 a         6908 c         7364 g         4085 t
ORIGIN      
        1 gcgcacccac cggcgaacgc ggcgaacatc agcaggacgg gcgcggccga gggccagacg
       61 ctgaagtcct gggtggtaaa accggtggtc gtcgcgatgg atacggcctg aaagaggccg
      121 tcccgccacg gggactccgt ggcgtccgga tagcttgcga gaatggcgca gaccagcgcc
      181 gcgactccta ccagaacgat caggtagaga cgaacctccg cgtcacgcgt gtacagaaac
      241 gggttgcgtc ggttccatgc catgaagtgc aggccgaaat tgatcccggc gagaatcatg
      301 aagaagatgg ccactccttc cacggccgcg ctgtcgaagt ggccgatgct ggcgtcgtgg
      361 gtcgagaagc cgccgatggc gacggtggaa aagctgtggc aaagggcgtc gaaggcgtcc
      421 atcccggcgg cgtagtaagc gagcgcgcag gatgccgtga tgccgacgta gagataccac
      481 agggccttcg ccgtctcggt gatgcgcggc gttagcttgg catccttgac cggccccggg
      541 gactcggcgc gatacagctg catgccgccg atgcccagca tgggaaggat cgccaccgcg
      601 agaacgatga tccccatacc gccgagccac tgaagcagct gccggtagaa aaggatggaa
      661 cgggggagct cgtcgagccc ggtcaggacc gtcgccccgg tggtcgtcag ccccgacagc
      721 gactcgaaag cggcgtcggt gaaagacgca tcgacgctct cggccaggta gatggggatt
      781 gccccgaaca ggccgagacc caggtagaag agcacggtga tgagaaaccc gtcgccgctt
      841 cgcaactcgc gccgggaccg tgcgaccagg gtcagcgcca ggcccgaaat cagggtgatg
      901 gcgaaagcca gggcaaacgt gcgggtggtg ctctccccgt agtagacggc caccaccacg
      961 ggcagcagga gcgcaatgct gaacagcatc agcagcgttc cggtcactcg gagaatgacg
     1021 gagaaatgca tcagaaaaag gtcagcccga cctggaacag ccgttccacc gccgccacct
     1081 gacgcttgtc cgccagaaac aggatcacgt ggtcgtcggg ctccaccacc acgtcgtcac
     1141 ggccgacgaa aacttccttg ccccggacca cggcgccgat ggacgcgccg gtcggcaggt
     1201 cgacatcccc gatcgcccgg ccaaccacct tggagttttt cgcgtcgccg tgggcgatgg
     1261 cctcgatggc ttctgcagca ccgcggcgca ggctgtgcac cttgaccacg tcggcccgtc
     1321 gcacgtgggt caggagctcg ctggtggtgg cctgctgagg cgagatggcg atgtcgatgt
     1381 cgccgccctg catcaggtcg acgtaggcgg gcttgccgat gagcgtgatc acctgtcgga
     1441 ccccgagccg tttggccagc aacgaggaca tgatgttgac ctcgtcgtcg ttggtgacgg
     1501 cgcagaaggc gtcgcactgc tcgatgttct cctccagcag aagctcccgg tcggtggcgt
     1561 cgatgttgag gaccacggac cggtcgagaa cctcggccag ctcctcgcag cggcgggcgt
     1621 tctgctccaa caccttcacc gagaagtaag gctcgatgct ctgggcaagc ttgacgccga
     1681 tgttgccccc gccggcgatc atcacgcgct tgaagggccg ctccacccgc ctgagctcgc
     1741 tcatcacggc gttgatgtgc tcggcggcag cgatgaagaa gacctcgtcg tctgcctcga
     1801 tcaccgtgtt gccctcgggc gtgatggcgc gaccgcgacg gaagatggcc gccacgcggg
     1861 tgtctatgtt cggcatgtgc tggcggagaa agcgaagctc ctgaccgacc agggggccgc
     1921 cgtagtacgc cttcacggcc accagctgga ctctgtcgtc ggcgaacccg agaacctgaa
     1981 gcgctccggg gtgctcgagc agctgtcgga tgttttctgt gacgacctcc tctgggttga
     2041 tcagcacgtc gatgggcatg tgctccccgc tgaagaaccc ttcccccttc agatatgctg
     2101 acgagcgaac ccgcgcgatt ttctgcggcg ttcgaaacag ggagtagcag acctggcagg
     2161 cgaccatgtt gacctcgtcg ctggaggtca ccgcaatcag catgtcggcg tcgtcggcgc
     2221 cggccttgcg cagcacgtcc gggtggctcg cggacccgaa gacggtcttg atgtccagct
     2281 tggtctgcag ctcctcgagg gcttcgatgt tgttgtcgac cagcgtgatg tcgatggatt
     2341 ccttggcgag gttctcggcc agcgttccgc ccacctgccc cgctccgaga atgagaatct
     2401 tcatgttggc tgccttgtta cacccgcttg gtcagaagag cgtagtagaa cccgtcggtg
     2461 cggctgtcgg tgggcagaag ctgccacccg tgagccctgg ccacgccggt agccagcgac
     2521 agagggcggg cctcggcatc gtcagccgct ttgaggaacg ccccgatcac ctcatcgttc
     2581 tcttccgcaa acagcgagca cgtgcagtaa agaaggttac cgccggggcg cagggtctgc
     2641 cacaggttag tcagcagccg tccctgcagt cgggcgtagt cgtcgaggtc cgaggctgcc
     2701 cgcaggagcc ttatgtcggg gtgccggcgc agggtcccgc tgcccgtgca gggggcgtcc
     2761 agcaggaccc gatcgaaggg ctggccatcc caccacctca ggtccgtggc atccccctgc
     2821 tgggcgttca cgttgtgatg gaggcgagcg gcctcggctt cgagccgttc caggcgtgcc
     2881 gtggaggtgt cgatggccag ggtggccgcg tccgggaacc gctcgaccag gtggaagagc
     2941 tttccgcccg gggccgcgca ggcgtcgagc accctggacc ccggctccgg agccaggatg
     3001 tctgcggcga atccggcgcc cgcgtcctgg atggccgcca gcccatcgtc gaagcctggc
     3061 agccgggccg tcggcatggc cgccgtcaga atgatgaact cgtcctgaaa gccctccgag
     3121 aaggcgatgc cggaatccgc gagccgctgt cggtagccgt ccgtcgcgac gagtctcctg
     3181 ttgatgcgaa cgcacatggg cgcacggccc atgttggcgc agaagatggc gcccgcccgg
     3241 tccggaaagt ctcgacgaat tcgttcagcc atccaggccg gatgctccga agcatccgaa
     3301 caatcgttcg gcagaccctt tcgctggatg tggcggagaa cggcgttgac cagacccgta
     3361 gcccagggct tgcgaagcgc ccggcacccg gcgaccgtct cgttgatggc ggcgtggtcc
     3421 ggtacgcggg cgtatgccag ttggtaagcg cccactaaca ttaaacagta gacgtcccga
     3481 tgcttgttcg ccagaggctt gcgcagcgcc ttgtccacac cgcgttgcag gcagaaaacg
     3541 tgtcgcaggg tgccgtagaa cgtgtcgcgg acctgcggtg tggacgggat gcgctccaga
     3601 gcctgatcgg acgtctgttt gtccctgagc acggcgagca gcaggcgcga cgccgtggcc
     3661 ggagcgcctt cagctgggct gggcaagctt tgtgccttct tcaaaatagg cggcgtagcc
     3721 gttgacagcg tcggctgcgg acaggggacg cccgcgcccg cgatcgagtt gcacggactg
     3781 gatgaccagg gtgctctccc tgcaggagac gcggataccg ttcctgtcag cggaaacgat
     3841 ccttccgggt tccccgccag ggtcccggat cgcccgcgcc tggagaatgc ggatgcgcag
     3901 gtcgccggag gcgcaggtgg cgggcaatct tggattcaga gctcggatct ggcgctcgat
     3961 cgcttcggcg gggcggttcc agtcgatgct ggcatcggcc ggcgtcagct tgtgggcgta
     4021 cgtcgccccg gcctcatcct ggggcgtggg gtcgaggctg cccagattcc ccaggcagtc
     4081 cagcaggcag cgggcgccaa gccgcgccag cgttgcttcg agctcgccgc cggtggttgc
     4141 gctttcgatg gggcacctct cctgctcgat gatcgggccg gtgtccagac cggcgtccat
     4201 ctgcatgatg gtaacccccg tctcctcgtc ccccgccatg atggctcgct cgaccggcgc
     4261 ggccccgcgc cagcgcggca gcagcgaggc gtgaacgttc atgcacccgt acctgggcgc
     4321 gctcaggatg gcctggggga ggatcagtcc atatgcagct accaccagcg tgtcgagctc
     4381 aagccccgcc agatccgccg cggccccggt ctccctcagc gtggccggct ggcgggtctc
     4441 gatgccctgc ttcgcggcca gggccttgac ggggctcggg agcgccttgc ggccacgacc
     4501 ggagcgccga tcgggctggg tgtacacgac caccggcgag tggccggccg tgacgagcgc
     4561 ttggaggatg gcggccgcga actcgggtgt gcctgcaaac ccaaggcgcg gcgcgctgcc
     4621 gcccgccctg gtcatgcgcg gacgcggcgt tgtttgtcca gctttttgcg aatccgcgtg
     4681 cgcttcaggt tggacaggta atccacgaag agcttgccct ccaggtggtc gcattcatgc
     4741 tggatgcaga cggccagcat gccctgggcc tcgagcgtaa aggactgtcc atccctgtcg
     4801 agcgcgctca ctattacctg ctcggagcgg atgaccggct cgtagaaccc cggaaccgac
     4861 aggcaaccct cctccgactc gatggcggcc ccctgcagcg aaagctcggg attgatgaaa
     4921 acgtgcggct cgtctcggtt ctcggaaacg tcgatcacga tgatccgttg gtggacgttg
     4981 acctggctgg ccgccaggcc aattccggga gcggcgtaca tggtctcgaa catgtcttcg
     5041 accagttgct gtacttgcgc gtccacctca gtcacgggtt tggcttgagt gcgcagcctg
     5101 gggtcgggat attcgagtat gtcgagggtt gccatggatt gttgtagcgc ctttgcggcc
     5161 tatgtgatgt gtctctactt ctgctgcaat tcaggctgat aacattgtga cgagtatatg
     5221 tttttcgctc ggaaacccgg ctcggtcgcg ggctcaagcc ttacccggcg acggttgagc
     5281 ggatgttaat gagccttcat ttggacttaa aggcctacaa ctcgaaatgg acagcatccg
     5341 gcggcaagag ttcgaagcga gccagcctcg gcgatcctct tcagggatgc ttccggcacg
     5401 actagcaggt acagctaaaa ggaaaggaat catgaggaaa ctgcgcatca ttttccaggt
     5461 tctgatcgga atcggtcttg ggatctcggc aagcgcatgg agtgccacgg acggcctggc
     5521 tcagtacctc aagccggggc atccggaggt ttacaccgtg gtcaagggcg acacgctgtg
     5581 ggatatcagc ggcaagttcc tggatcggcc ctggctttgg ccggagatct ggcggataaa
     5641 cccccagatc gataatcccc atctcatcta cccgggcgat cagatcgcgc tcgagtgggt
     5701 ggagggacag ccccggttgt ctctgagaag gggtgacgcg gcgcgaacct acaagatgac
     5761 gccggcggat tcggtgagcc tgcaaccgca gattcgctcc accccgctgg agtcggccat
     5821 ccccaccatt cggctggagg ccattcaggg gttcctggtg cagaatcggg tggtggatcc
     5881 caaggttctg tccagcgcgc catacgtcgt gcagggcgaa aacgagcgcc tggttctggg
     5941 cgccggcgac cggctctacg tgcggggcgt gctgccggac agcgaaagct tcaacgtcgt
     6001 ccgcaaaggg cccctttatg tggacccgga gacgaaagag gtgcttggcc aggaggctac
     6061 ctacatcggg ctgggcaggg ccgtggcgca ggaagacgat attgccacca tgtcgctgca
     6121 gagcacccgc gaggaagccc agatcggtga ccgagtgctg ccaaccgaag agcggcgggt
     6181 tgactccacg ttcttcccga gcgcgccgga cggggacgtc aacggccaga tcatttccgt
     6241 gttcggtggc gtgacccagg tcggccagta cgacgtggtg gtgctcaatc gcggcgagcg
     6301 ggagggcatc gaggtcggca acgtcatggc gatctacaag cgcggcgctc ttgtcaagga
     6361 ccgtatcgcc aatcagacca tccggctccc cagcgaacgg gccggtctgc tgatggtctt
     6421 ccgggcgttc gagaagctct cttacggcct ggtgctcgag accgagcgtc cgctcgccgt
     6481 caacgacgag gtacgtaatc cctgatttgc gctacggtgc ggcgaaatcg caccgctgac
     6541 cggccgccgg gctgaccggc ggcaatcagg gtcttcaata gaatcgcccc tcaagtcgag
     6601 gggcgatttt tttatggggg acctggctgg ggacgtggag tcggagaacg cggcggcggc
     6661 ctgccttgcc atggagcttg gtcggccgcg ggcacgggcg gcaatcgccg ctcatggcaa
     6721 tgcgacctcg gcggtcgaag ctcgggcagc gtcccgggat tggaaccccc gcctggtacc
     6781 ccaacggctg ctcgcggatt gtgcccggca cgggatctcg ctgctcggcg gggaggatgc
     6841 gtgctttccc gagctgttgc ggcacatccc cgacccgccg cttgcactgt tctctctcgg
     6901 cgcgctgccg ggacgcgatg ggactgccgt tgccgtggtg ggcgccaggc gttgcagccg
     6961 gctcggccgg gaggtcgcct accagctcgc ccggcaaatc gcgacggcgg gcgtcagggt
     7021 ggtcagtggg ctggcgctcg gtatcgataa agccgcccac gaaggcgcgc tcgatgcccc
     7081 tggggccggt cggaccgttg cggtcttggg ctgcgggctt gcccggatct atccgcggcg
     7141 caatcaggcc ctcgccgagc gcgtggtggg cgagggcggc acattgatca gcgagtaccc
     7201 gccggaagtg ccccccgcga agcatcggtt tcccgagcgc aacaggctga tcagcgggct
     7261 ctcccacggc gtggtcgtgg tcgaggccgg cgatcgcagc ggctctctga tcaccgcgcg
     7321 actggcactc gagcaggggc gggaggtaat ggccgttccc ggaccggtga gcggcgggac
     7381 gagtcgaggc tgccatcgcc tgctgcggga gggggccgcg ctggtggagt gcgccgagca
     7441 tgtcttcgaa gcgctgaacc tgacccgccc cacccccgga tccgccgaga cggaacaaag
     7501 ggcgggtttg gacctgggcg agcctctgaa cagcgtgctg tcggcggtgg agcctgtgac
     7561 caccaccatg gaccagctgg tcgccgtcac cggcatggct cccgaggccc tgtcggtgtg
     7621 tctggtcgat ctggagctcg cgggatttgt cgagcgggtc cacgagggtt atattcggcg
     7681 acctttttcg ccggggatat aaatttccgg ctcaggagga aaaaacatga gctttgtggc
     7741 cattttgatg ggttccaact ccgactggcc ggtgatgcag agcaccaccg aggtgctcgg
     7801 cgccctggac gtggagttcg aggtgcgggt cacctctgcc caccggaccc cggcggcgac
     7861 catggagtac gtgcaggacg cgacccagcg aggatgcgcg gtcttcatct gcgccgccgg
     7921 catggcggcc catcttgcgg gcgccgttgc ggcgcacacc acccgaccgg tgatcggggt
     7981 gcccatcgac agcggcccgc tttccggatt cgacgcgttg ctctccacgg tgcagatgcc
     8041 gggcgggatc ccggtggcga ccgtggccgt gggcaaagca ggggcgaaga acgccggtta
     8101 cctggccgcg cagatcctgg gactgggtga tgcggccctc gccgaccggg tcgcctccga
     8161 gcgggcggag aacgcacgca aagtccagtc ccagaacgag cagctgcagg aagaactcgg
     8221 ccgctgatga cttccgtgca cgtggcgcgc gccgtccggg ggcttaaggc tggcggcgtc
     8281 gtgctgcacg cgacggaggg tgtctggggc cttgcctgcg atcctttcga cgtggaagcg
     8341 gtggctcgcg tactggtgct caaaggtcgc ccggtggcca agggcctgat ccttgttggc
     8401 gggaacatgg atcacttcgc tgaggagctc gacctgctcg agccggctgt ccggtctcag
     8461 gtcgaaagca cctggcccgg tgcggtgacc tgggtccttc ccagcacccg ctttccggag
     8521 tggatcagcg gcggtcggga aacggtagcc gttcgggtct cgggtcatcc ccaggtgaga
     8581 gcgctgtgcg aacgctttgg cggagccctg gtgtccacct cggcgaaccc caccggcaga
     8641 ttgccggcca ccagccagat cagggcacga gcgtacttcc aggggcgcgt ggattacgtc
     8701 ctccccggcg aggttctcaa cgcgggcgcc cccagccgga tccagaccct gggtggcgag
     8761 gtgctgcgcg gaggaggccc gtgagcgccg gaaagccggg ggaaccggat cgctacgcgg
     8821 tgatcggcca tccggtgtcg cattcgctct cgcccgcgat ccacgccatg ttcgcagagc
     8881 agaccgggca gcatctggcg tacgcaaagc tgccggcccc gagagacggt tttgcccgga
     8941 cggccagcga cttctttgcc ggcggtggga gcgggctcaa cgtgaccgtg cctttcaaac
     9001 aggaagccgc cgggtgggtc gatcttctgg aaggggccgc ccgggaggcg ggggccgtca
     9061 acaccatctc ccttgaatcg gggcagacca aaggcttcaa caccgatggc ctgggtctcg
     9121 tcagggatct caccgtgaat cacgggcttt ccatctccgg tgcccgggtg ctcgtggtcg
     9181 gtgccggcgg cgccacccag ggggtggtgc gtcctctgat cgcccttggc ccccaagcgc
     9241 tgaccgtcgc caaccgcact gtctcgaaag ccgctgaact ggtcggttcg ctgggctctg
     9301 ccgccggccg cgcaagggtg gcagcctgcg cgccagaggc ggcgacgggt ccgttcgatc
     9361 tggttatcaa cgccacctcg gcaggtttgg aaggtcacgg cgaggtcgtg cccgccgacg
     9421 cggctgcggg ggcagtgtgt tacgacatgg tttacagcac gggggcgggc gctgccacgg
     9481 ccttctgcga gtgggcgctc ggcgtggggg cggttcgcgc catcgacggg ctcggcatgc
     9541 tggtagagca ggcggccgaa gcctttctga tctggcgtgg ggtcaggccg gacaccgcgc
     9601 ccgtcatcga cgcgctccgt cgcgttgcgc cgccggcgta ttgaacagga atggagcttt
     9661 cggcttgacg atcaagaagc gattcattgc gggggccgtt tgccccgatt gcggcgcgca
     9721 ggatcgcctg gtcgtcgagg aaatgcctga cggtaagcgc cgccgctgcg tgagctgcgg
     9781 attcaccgac gtcagcgcgt ctgagccgga agtggctttg ccgacgcggt ttgcgcgaag
     9841 gggcgtcgat tcggagcagg cgcgccccgt gcgtctgctg ggcgaaggcg attcagactc
     9901 ctgatcgccg ggcacttttg ttcgactaag gcgttgaaaa cctcacaatt tcgactgcct
     9961 tcgttgtgat ctggcggcgc gctgctataa tgcgcccgct cttcggttgc tctgccgacg
    10021 aagagcttcc aggaagcacc ttcaagcccg ttttcgggca cgaccaagag gtcacggtct
    10081 gaccgccgtc cgccccccaa ttgggaggcg gacgctttga ctcgaccggg tcgctggtgc
    10141 gccgaggcgc aactctttag cggctccctt atcaggcgac cgacggtcgc cgggggtctt
    10201 tgttgcttcc gaagatgcca caacctaacg caagggtgag caggtttgaa acattactgg
    10261 catgggctgc cgacgtgcct gatgggcctt ggactggccg ccgctgcgcg agcggacgag
    10321 ctgaacatga cgccgggagt gacgcagatc agtcgctcca tctacgacct tcacatggtg
    10381 atcttctgga tctgcgtcgc catcggcgtg gtggttttcg gggtcatgtt ctggtcgatt
    10441 tttcagcaca ggaagtcgcg gggcgcgcag gcggccagct tccatgaaag cactcgcctg
    10501 gagattgcct ggacgctgat tccgatcctc atcctcatcg gcatggcctt tccggccacc
    10561 cagaccctcg tcgccatgta tgacacgggc ggagaggaca tggcggtcga ggtgcgtggc
    10621 tatcagtgga agtggcagta caagtatctg gacgaggact acaacagcac gttcagcttc
    10681 ttcagcaacc tggcaacctc cagggacgag atcgaaaacc gtgtggtgaa gaacgacaac
    10741 tacctcctgg aggtcgatga gccgctcagg atcccggcga atcggaaggt gcgctttctc
    10801 attacctcgg aggacgtgat ccacgcgtgg tgggttccgg atttcggcat caagcgagac
    10861 gcgatcccag gcatgctcaa cgagctgtgg accgtcgtgg agacgccggg cgtttaccgg
    10921 gggcaatgca ctgagctttg cggtatggac cacggcttca tgcccatcgt cgtcgaggtc
    10981 atggcggagg aagactacga cgcctggtat gcggaccggg tgcgcatgga ggaagcgcgc
    11041 cacgaagcgc tctccaagac gtttacgccg gcggagctca tggaggaggg ggagcgcgtc
    11101 tacgcgacgt tctgcgcaag ctgtcatcag gcaaacggca aagggctgcc accggtgttc
    11161 ccggcccttg ccggaagccc tatcgccacg gggccgctca gcgaccatct ggacatcatc
    11221 gtcaacggcc gtgccgggac gagcatgaat gcctttggca accagctgga cgcggcggag
    11281 atcgcggccg tgactcatta cgagcggaac gcctggggta acgacgtggg cgacgttacg
    11341 cagcccatcg atgtcatcga gttcatgtct gggaaggaag ccgaggaaac gccatgagtg
    11401 aagccgtact gactcatcac gacgaccatc atcacgggcc cgcttccgga atcacccgct
    11461 ggctgttcac cacgaaccac aaggacatcg gtaccctcta cctctggttc agcttcctga
    11521 tgctgctcat cggcggcgcc atggcgctgg tgatacgtac ggagctgttc cagccggggc
    11581 tgcagttcgt agaacccgcc ttcttcaacc agatgaccac gaaccacggc ctgatcatgg
    11641 tcttcggggc catcatgccg gccttcgtcg gtctggcgaa ctggctgctg ccgatgatgg
    11701 tgggcgcgcc cgacatggcg ctgccgagga tgaacaacct gtcgttctgg atcctgccgt
    11761 tcgccttcgg catgttgatc tcgacgctgt tcatggaggg gggcgggccg aatttcggat
    11821 ggacgttcta cgcccccctg tcgacgacct atgcgccgga cacggtgacc tatttcattt
    11881 tcggcgttca cctgatgggt gcgtcctcca tcatggggag catcaacatc atcgccacca
    11941 tcctgaacat gcgcgcgccg ggcatgacgc tcatgaagat gccgctgttc gtctggacct
    12001 ggctgattac cgcctatctg ctgctggccg tcatgccggt gctggccggg gcggtcacca
    12061 tgatgctgtt cgacatccac ttcgggacga gctttttcaa cgcggccggc ggcggtgacc
    12121 ccgtgttgtt ccagcacatc ttctggttct tcgggcaccc ggaggtctac atcatgatcc
    12181 tgcccgcgtt cggcgtcgtc tcccagatca tccccacgtt cgcgcgcaag cctctgttcg
    12241 ggtacgactc gatggtctac gccaccgcca gcatcgcctt tctgtcgttc atcgtctggg
    12301 ctcaccacat gttcaccgtg ggcatgccgc tggccgggca gctgttcttc atgtacgcaa
    12361 ccatgctcat cgcggtgccc accggggtaa aagtgttcaa ctgggtttcc accatgtggc
    12421 gtggatccat gacgttcgag acgcccatgc tgttcgccat cgcgttcgtc gtgctgttca
    12481 ccatcggcgg gttctcgggc ctgatgctcg ccatcgcccc cgcggactat cagtatcacg
    12541 atacctattt cgtggtggcg cacttccact acgtgctcgt tccgggcgcg ctgttctcca
    12601 tcatcgccgc cgtctactac tggctgccca agtggtgcgg ccggatgtac gacgagaccc
    12661 tgggcaaggt gcacttctgg ctgtcgttca tcggggtcaa cgtgacgttc ttccctcagc
    12721 acttctcggg cctcgccggc atgccgcggc gaatccccga ctacgcgctg cagtttgcgg
    12781 acttcaacat ggtctccagc gtcggggcgt tcatcttcgg tttctcccag ctggtcttcc
    12841 tctacgtggt gatcaaagcc atcaggagcg ggcggccggc caccgaccga gtctgggagg
    12901 gcgcccatgg gctggaatgg acgctgccct cgccggcgcc ctaccacagc ttcacgactc
    12961 cgcccaccgt caccgaggcc gaagcccact cctgatgggg gaggggctaa tcgggtagga
    13021 ggcagcgaag ccgcatgcac aagagcagca aaggcatcgt atggaaactc gggctgatgg
    13081 tcgtgggcat gttcggcttc ggcttcgccc tggtcccgct ttacgatctg ctgtgcgagg
    13141 tcaccggcct gggcggcagg accggtgggc cctacgctta cgacccggcg gtcgtggagg
    13201 tggatcgctc ccgactggta aaggtcaacc tgctgaccaa taccaacgat ggcatgccct
    13261 ggcggttctg gtccgaggtg gggggcatgc gcgtgcatcc tggtgaggcc aaggacgtga
    13321 agttctacgt caagaacacc accgataggc gcatggtggg ccaggcggtc ccctcggtgg
    13381 tcccgatcac ggcaaccgac tattttcaca aaactgaatg cttctgtttc gaaagccagg
    13441 tcttagagcc tggcgaggaa atggagctgc cgctgcgatt catcgtcgat gcggggctgc
    13501 cgaagaacgt gcagaccata tcgctcagct acgccctgtt cgacgccacc ggtttcgcgg
    13561 cggcggagta acccgacacc taaaagggag agaacggcca tggccagctc atcagaccaa
    13621 gccacttatt acatccccca cagcgggtgg tacccgatct gggtggccgt cgggtcggcc
    13681 ctgcttctgt tcgggctggg ctcctggctg aacgatctca aggccgagac ggagccgagc
    13741 cgcgcgctgt tctataccgg ggccgtcatc ctgtcggtgg tgctgttcgc ctggtttgca
    13801 aaggtggtca acgagaatct cgaggggctc acgagccccc agcttcatcg ttcttacatc
    13861 tggggcatca gctggttcat cttctccgag gtgatgttct tcttcgcctt cttcggcgcg
    13921 ctgctctatg cccgggtgct ctcggttcag tggctcggcg gcgagggcgc gaaaggattc
    13981 accggggagt acctctggcc cgaattcgag gccgtctggc cggtgatcag caatcccgac
    14041 ccggcggtgt tcgagaaccc ccggggtgat atggcctccc ccgggctcct gagctggggc
    14101 ggctacctgc cgttctggaa cacggtcatc ctgctcacct cttccgtcac ggtgcatttc
    14161 gcccacacgg cgatcaagaa cgctgaccgg accaggctgc tgaggtggct cgggctgacc
    14221 gtcgcgctgg gcgtggtctt tctgtttctg caggtggaag agtacgtgca cgcgtatcag
    14281 gagctgggcc tgactttgtc cagcgggatc tacggctcga ccttcttcct gctgaccggc
    14341 ttccatgggt tccacgtcac gctcggcacg ttcatgctct tcgtgcagct ggttcgtgcc
    14401 ttcagggggc actttcaacc caaggactgc ttcggcttcg aagcgtcttc ctggtactgg
    14461 catttcgtgg acgttgtctg ggtctgcctg ttcctcttcg tctacgtatt ctgaagccgc
    14521 tcagtcgccg gaggtctggg actgatgcca cggggcgttg ctgcccatcg agagctctcc
    14581 cgtggcaaag ccgtacagga cggtgagtag cagcgcgccc gccagggtta ttcttatgcc
    14641 cagggcataa agcgctcgct ttgattccgg gcgttcgctg tccctgaaca ggaaggccag
    14701 gctgctgaag aggctcagca ccacccctgc caggaggaag atgatcacga tctttatcat
    14761 ggaggatgcc ggcggcgcga gaagctgcca gagtaacgca tccatccggc gggattaacc
    14821 gcgttggggg ctgcgcgcag atgggcgccc ggttggcgca tgtcgttggc cgtgacgctg
    14881 ctcgtcccgc tgatgctcgg gctgggcttt tggcagctcg atcgcgccga tcagaagcgc
    14941 gcacatcagg acacgctgtt ctcgcgcctt gcctcagctc ccgtggcggg ccgtgatacc
    15001 ctcgccccgg agccgttcca gcgggttcgg ctggaagggc ggttcgaggc cgatcgctat
    15061 tttctggtgg ataaccaggt ggctgacggc gtggtgggct actgggtggt ccagagtttt
    15121 tcaactccgc agggcgtgcg ctggctggtc aaccgcggct ggattgccgg cggcgatcgt
    15181 cgcgaccaac tgccccgggt gtcggcgccc acgggcacgg tggtcacggt gggggtggcc
    15241 tggccggacc tgggcctggt cccgcttctg gacgaggatg actggcccct ggcctggccc
    15301 gtccgcgtgc agcggctcga tgtcgcaaga atggctgaac ggctggagga tgccgtgccc
    15361 gttgaaatcc ggctggaaac ggcccagccc ggcgccctgc gtgcggcgcc gctgacggtc
    15421 ggcctccagg cggagcgcca cgaaggttac gcggcgcagt ggtttgccct ggcaggcgtg
    15481 ctgatcgtcg ggtacgcggt ctacgggctg cggggcgcgc agcgcgccag gcgattgaac
    15541 ggacgaacgg atgagagtcg atgacccaag ctgaagacaa aaccggggat cagagaggcc
    15601 gcgccgggcg cagccagctg gtggtgatcc tgctggtggc cggcttttcc atggcggggg
    15661 cgtatctggt cttctttctc gccagcagcg gtggcgtctg gggcaccacg aacaacggcg
    15721 agttcgtgac cccggcgatg accatggagg ccctcgacct gcgcgacgcc gcgggcactt
    15781 ccgcgacggc agacgggcat tggtggctgt ggacggtcgc gcccggcggg tgcggcgagc
    15841 agtgccggtt cgcggtggag cggctgcggc aggtgcacgc gctgctcaac aaggatgcat
    15901 cgagggtcag gcgcgcgctg gtcacctcga cgccggggct cgagccgccg ccagggctgg
    15961 atgtctccga tctcgagagg ctcgtcggcc cggcggacgg gctggcttcc ggtatctaca
    16021 tcgtggatcc tatcggaaac ctcgtgctcc gctacccgct gtccgacgct ggcgaagcgg
    16081 tgctggagga tctgaaacga ctgttgaagg tgtctcaaat tggctgacaa gaccctcctg
    16141 acgctctcga aggccggcgt ggcgctggcg atgctggtcg tcgtgctggg agcctacacg
    16201 cggttggcgg atgcgggcct gggttgcccg gactggcccg gctgctatgg attcctcacg
    16261 gtgcccgagt cggcggaaga catcgcccgg gccgagaccc tctttccgga cgcgccggtg
    16321 gagcgggcca aggcctggtg ggagatggtt caccgttact tcgccgccgg cctcgggctg
    16381 ctggtcctgg tgatggccgg gcgcgcctac ctgcgtaggg agcagggggg gccggtcaag
    16441 ctgccgttgg cgctgctggc gctggtcatc ctgcagggcg ctttcggtgc ctggaccgtg
    16501 accatgaagc tttggcctca ggtggtgacc gcccaccttc ttggcggctt caccaccctg
    16561 agtctcctgt ggctgctctg tttgcggctc ggtgtcggca gtggctggga ggtgtccgga
    16621 aagctcagga cgccggccct gatcgcgctg atcgtgctgg tgatgcagat cgcgctcggc
    16681 ggttgggtga cgtccaacta cgccgccctt gcgtgtccgg acttccccac ctgtcacggc
    16741 gagtggctgc cggaaatgga tattggtgag gggttcaact tcgcccagtc cgtgggcccg
    16801 aactatctgg gcgggatgct ggagagtgaa gcccgcgtgg cgatccagct cggccaccgc
    16861 ctgggtgcat tggtcgccac cctggccgtg ggctggctgg gtttgctgct gctgcgcacc
    16921 ggctcgcccc tcggctggcc cgttctcgtt cttctggccg tgcaggtggt gctgggaatc
    16981 ctcaacgtgt tgctggcatt acccctgagc aacgccacgc tgcacaatgg tttcggcgcg
    17041 gtattactgc tggccttggt tacagtaaac tatcgggcct acttgggggg gatgagaagt
    17101 tgagcgaagt cagcctgcaa gccgccagct ggagcgacta ccttgaaatg accaaaccaa
    17161 gggtcgtcct gctgatgctg ctctgcgcgc tggtcgggat gttcctggcc gtgccgggca
    17221 tggttcccgt ggacgtgctg gtattcggcc tggtcggcat tgccctggtt gctggctctg
    17281 cggcggtggt caaccacatt gccgacgctc agatcgacgc gcgaatggcg cgcacccagg
    17341 cgaggccggt ggccacgggc agggttggtt tgctccaggg actgctgttc tcagccgtcc
    17401 tgggcgtgtc gggaatggtg gttctctact tcctcgtcaa cccgctcacg gcctggctga
    17461 acctcgcttc ctggatcggc tacggcctga tctacaccct ttatctcaag cgcgccacac
    17521 cccagaacat cgtcatcggt ggcctgttcg gggcggcccc gcccctgttc ggctgggcgg
    17581 cggtgagcaa cagcgtggag ccgggcgccc tgctgctggt gctgatcatc ttcgcctgga
    17641 ccccgcccca tttctgggct ctggcccttg accgcaagga ggagtacgcc tcggtggacg
    17701 tgcccatgct cccggtcacc cacggggaac gctacacgag gctgcacatc ctctattaca
    17761 ccctgattct ggtcgccgtc agcctgcttc cgttcgtgat aggcatgagc ggctggctat
    17821 acctggctgg ggccactgcc ctgggtggcg ggttcatcta ctgggccgtc gtcctgcttc
    17881 gcaaccgcaa cccgcgggca ccgctggaaa ccttccgcta ctccatcgtc tacctcggcc
    17941 tgctcttcgt cgccctgctc gccgatcact acgtcttcgc cgaccacctg gtgggatcga
    18001 gcctgcagtg agatccgtta cagcgacggt gctgctgtgc ctgtcgctgg ccgcagccgc
    18061 cgtaggcgcc ttcgtgtaca gcgtcctgcg cgagccggta ctcagcgatg cggagcttcg
    18121 cgatctcggt gtgttcgtgc tgcctcgccc gcgcgccatt ggcgcgtttc agctggagag
    18181 cacccggggc ggttccttca ccgccgccga ccttcagggc ggttggtcgt tcctcttttt
    18241 cggcttcacc aattgtcccg acgtgtgccc aaccacgatg gccgtgctgg cccaggctga
    18301 gcgggagctg cttgcaggct caccggagga tgccgaccgc ttcgccgggg tgctggtttc
    18361 ggttgacccg gaaagggacg acgtgaggac gctgggtgag tacacggccg ctttctcccc
    18421 ccgcttcttc ggtgtgaccg gacctttcga cgagctcgcc tcgtttgccc ggcagctgaa
    18481 cgtgggattc ggcaaagttc ccgctccaga agggtctttc agcgagtatc tggtcgatca
    18541 cggcgcccag atcgtcatca tcaacccgca gggccactat cacggcttca tccgcgcgcc
    18601 ccacacggcg gatacgatcg tgcagacctt tgcgtcactg gccgcgcggt tctgagcgcg
    18661 gcctgcccgc gtcgccggct ggcggccggt ttccgccgct ctgtccgtcc ggggccggtg
    18721 tgatcggccg gctgttacgc ccggccggta agctcacccg gtgagcccag ccggcaagcc
    18781 cgaaacggct tcaggtgagc agccgttcgt ccaggtagaa aaagtgcgcg gcgtgctgct
    18841 cgacgacttt ctcgagctcc aacggcgtgt tgaagtgcag ccctggggac acctgacggc
    18901 cctgagcgaa cgccaggcgc ttgatccagc cgttcaaagc cgcgtacagg gtaaaacccg
    18961 agaagaacca gagctcggct cgaacgaggg ggcgcagttt ctgatacgcc cgaaggaagc
    19021 ccagcgttct ctcggggtcg agcatgccgt tggcgtcaat gcaccagtcg ttggcggcaa
    19081 ccgccagatc gtagacgagg aacccgctgg acacctggct gaacccgccc acgccgctca
    19141 gcccgtgctc gttgaacagc acgttgtcac ggcagatgtc accgtgaatg gccccggcag
    19201 gcagccccgc cacgtcttcc cggtccagca tggagactac ctgctggacg gttgcttcca
    19261 tgagcctcgc gctcggatac gagagccggc cgtcgagagc ggccgacgcc tcggcgagcc
    19321 acctctggtc ccaggcccat gccggcagcg gtccgaggtt gccgcccgct tcgtgaaaac
    19381 gagccatgaa ccggcccaat gccgctacct gccggacggt agggttgtaa acgtggcggc
    19441 cgggcaagcg ccgggtgagg atcaccgttt cgccgttcaa cgtgtccacg ggttgcccat
    19501 ccagattcgg gatcgccttt gccaccggaa ggcctgcccg gtcgcaggct tccaaagcgg
    19561 cgagctcggc ctcgaatcgg ggcgcctgca cccgcaataa caggtagtcg tgctcccttc
    19621 cggcccgccg ggttctgacg cgataagcgc aacggctggc agctgagcct ggccaggccg
    19681 agatgagctc gccgaccccg tagcgcgtca gggtctcttc gattcccggg tggccgggct
    19741 cggtgatggc gttcattggc tattccagct gtccagcatg gccgtatcag aactcgacga
    19801 tgacccagct gggcagcagc atgtcggcgc gctcaagatc gccaaagccc gtcgtcgagt
    19861 ctcttggaac caggtagtag ggttttccca ggcggggaac gatcttgacc attctgagct
    19921 gtcccgcctg gcgaaactcc aggatggttt tgtcctcctg cgcgatgatg gtgatgtccg
    19981 ggccctgcag cccgtcctgc gcttcgcttg ccagcgcggt gggcggcagg gtcgcggcag
    20041 ccgccatcgc cagcactgct gcgcctttgc ggcccgtctg tgatagtgtc cggagcgagc
    20101 gttgcacagg gggatgttga ccgaaacggc tgtgcaaggc aaagcatccg catcataaaa
    20161 aaggtcgaca catggccagc gacgaaaccg ccggggcggt aagcccgccg cttcttctcg
    20221 tggacggatc gtcctacctg ttccgggcgt accacgcgct gccggagttg aaaacgagca
    20281 ccgggttgca taccggcgcc gttcgcggcg tcatcggcat gctgcgccgg ctcgccaagg
    20341 attaccggga aagccccatc gccgtggtct tcgacgccaa gggcaaaacg tttcgggacg
    20401 acatttatgt ccagtacaag gccaaccggc cgcccatgcc cgatgatctg agtgagcaga
    20461 tcgagccgat tcatcagatc atccaggcca tgggcctgcc gctgatcgtg gtcccgggcg
    20521 tcgaggcaga cgacgtgatc ggcacccttg ctgcggaggc caccaggaag cggcgcgaca
    20581 ccgtcatttc gaccagcgac aaggacatgg cccagctggt gaccgagcac gtgaccctgg
    20641 tcaacaccat gagcgagacc accctggacc cggagggcgt ggtcgacaag ttcggcgtgc
    20701 cgccggagtt gatcatcgac tacctggcgc tgatggggga cagcgtggac aacattcccg
    20761 gggtgccgaa ggtgggcccc aagacggcgg caaaatggct caacgaatat ggtgacctgg
    20821 acggcgtcgt tgcgagcgcc gagcagatca agggcaaggt gggcgagagc ctgcgcgatc
    20881 atctcgacca gctgccgctg tcgaaggcgc tcaccaccat caaatgcgac gtggctctgg
    20941 aagtcggcat cgacgatctc gcccctcggc ccgtggacga agatcgcctc aaggggctgt
    21001 tcgagcggct ggaattccga agctggctcg ccgaggtggg aggtccccag gaggatgcgc
    21061 ctgccccggc agcggcctcg gccgaagccg aaggggaata cgaatgcgtc agctccgcgg
    21121 aggcgctgga cgaatggatc gagcggctgc gcgcggcccc gctgttttct ttcgacaccg
    21181 agaccaccag ccttaactac atgctcgccg agctggtggg ggtgtcgttc agctgcgagc
    21241 cgggcaaggc cgcctacgta ccgctggccc atgattatcc cggggcgccc gatcagcttg
    21301 gcgtggcagc cgcccttgac cggctcaaac cgctgctcga ggacccggcg gtcatcaagg
    21361 tggggcagaa cctcaagtac gacatgagcg tgcttcaacg ctacgacgtg cagctcgccg
    21421 gagtgggttt cgacaccatg ctcgagtcct acgtgctcaa cagcaccgct tcccgtcaca
    21481 acatggacga cctggcaaag aactatctct cccgggagac cattcactac gaggacatcg
    21541 ccggcaaggg cgccaagcag ctgaccttca atcaggtacc cgtggaacag gccgtgtcgt
    21601 acgccgggga ggatgcggac atcacgctgc agctgcacca gaccctctgg cccaggctcg
    21661 agaaggagcc gcgtctgacg gaggttttca gggagatcga gatgcccctg ctgtccgtcc
    21721 tctcccggat ggaacgcaag ggcacgctgg tggacggcgc cctcctcgaa gcccacggcc
    21781 gcgagctggc cgagcggatg cggggcatca cggaggaggc ctggacgctc gccggcgagg
    21841 aattcaacct agactcaccc aaacagctgc aggcgattct tttcgacaag ctcgagctgc
    21901 ccgtgctgaa gaagacgccg aaaggtcagc cctccaccgc cgagcccgtt ctggtcgacg
    21961 tggcccggga cttc
//
LOCUS       DBNHKV010000450         7563 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1196538, whole genome shotgun sequence.
ACCESSION   DBNHKV010000450 DBNHKV010000000
VERSION     DBNHKV010000450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7563)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1196538"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1958)
                     /gene="uvrB"
                     /locus_tag="AC16PP_14825"
     CDS             complement(<1..1958)
                     /gene="uvrB"
                     /locus_tag="AC16PP_14825"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR6482952.1"
                     /translation="MVKAVAEFEVVSDYEPAGDQPTAIAGLVEGLVDGLSHQTLLGVT
                     GSGKTYTVANVIEAVQRPTLVLAPNKTLAAQLYGEFRDFFPNNAVEYFVSYYDYYQPE
                     AYVPASDTYIEKDSSINAHIEQMRLSATKALLERRDTIIVASVSAIYGLGDPQSYLRM
                     VLHLDRGDRVDQRYVLQRLAELQYTRNDMAFQRGSYRVRGDVVDIFPAESEKEAVRVE
                     LFDDEIENISVFDPLTGEIVNRVPRYTVFPKTHYVTPRERILAVLEDIKEELQDRLAY
                     LQKHHKLVEAQRLDQRTRFDLEMIRELGYCSGIENYSRYLSGREPGEPPPTLFDYLPK
                     DALLVIDESHVTVPQIGGMYKGDRSRKETLVEYGFRLPSALDNRPLRFDEWERMSPQT
                     IFVSATPGPYEAERSGAVVEQVVRPTGLVDPDVIVRPASTQVDDLFGEIRRTVENRER
                     VLVTTLTKRMAEDLTEYLDEHGVRVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLL
                     REGLDMPEVSLVAILDADKEGFLRAERSLIQTIGRAARNVNGRAILYADHETGSMRKA
                     IGETERRRAKQTAFNEAHGITPRTIEKQIADVMEGARAPAPGTVRRKGAGPAHGSAER
                     IEIPEDPKAFGRLLSELEERMLEHARNL"
     gene            complement(1965..2339)
                     /locus_tag="AC16PP_14830"
     CDS             complement(1965..2339)
                     /locus_tag="AC16PP_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482953.1"
                     /translation="MKVVVKTDEYTIYQRRDERYAVKDATKAWVNGDAKAAILVQHEL
                     ITAALPAAPLEEPEADAGEPAAEAAAAEEVTGAEESAAEESEPAEAAAPAEEAAAAEV
                     AAEDEAGEPASAEEGEEEPKPE"
     gene            2514..4367
                     /locus_tag="AC16PP_14835"
     CDS             2514..4367
                     /locus_tag="AC16PP_14835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR6482954.1"
                     /translation="MVEEKTSYVIRFAGDSGDGIQLQGQQFTTASALAGHDLATMPDF
                     PAEIRAPAGTRYGVSAFQIQFGGDRVTTPGDAPDVLIAFNPAALVVNLSHLRPGALVV
                     VDRQAFTDQRLKRADLTSNPLDDGTLDGFELLDIDISDLTHQAVDALGLGAKDSLRCK
                     NFWALGLVLWMFDQKRDATVSWIEAKFAGNEQVRSANLAALNAGHAFGETAELGPKLR
                     QHAAPEASLASAEYRTLTGAEALALGLVAAGELADTELMFCSYPITPASSLLHQLTGL
                     EDAGVATFQAEDEIAAICSAIGASYAGKLGVTSSSGPGIALKTEAMGLAVAAELPLII
                     VNSQRAGPSTGMPTKTEQSDLYQAVYGRNGDTPLPVVASRSPADCFDTAIEAVRIALR
                     HMTPVILLTDGYIANASELWPLPDFDHYEPIETHKPDPSTDPRHSVFSRDPKTEARLW
                     ATPGDKRFVYRVGGIEKDIHTGNISYEAQNHQEMTNMREAKVASVASFIPDQQVDSGT
                     DAGLAVVAWGSTYGTIYQAVRECVAEGLAVGHIHLRHLNPLPPNLPTLLAQFDRILVP
                     ELNNGQLATLLRDKLVLPVEQHNQVNGQPFAVADVKQAIARYAPKRIKEAG"
     gene            4371..5381
                     /locus_tag="AC16PP_14840"
     CDS             4371..5381
                     /locus_tag="AC16PP_14840"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR6482955.1"
                     /translation="MAELTFKDYATDQEVRWCPGCGDYSILKAVQRALADAGAAKEQT
                     VFVSGIGCSSRLPYYVDTYGFHTIHGRAPAVATGVKLANPDLDVWVITGDGDALSIGS
                     NHLMHALRRNVDLNILLFNNEIYGLTKGQYSPTSRIGTRSPSSPKGSVDSPVQPGLFA
                     VGAGATFIARAVDVDQKVLPGILNAAREHKGAALVEILQNCIVYNKDVFEDVVAKKTA
                     ADTQVVVEHGHPLVFGKERDKGLRLNPQTLALEAVTLGENGVSEADLVVHDETNTALA
                     HMLLSLAPPNPVAMGIIHRRRAPAFDAAYWSSKPTGRSARVAELLRHGNVLDHRRSPQ
                     GR"
     gene            5545..6273
                     /locus_tag="AC16PP_14845"
     CDS             5545..6273
                     /locus_tag="AC16PP_14845"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR6482956.1"
                     /translation="MAKHEEIAESLTRDILSGQYRTGERLPSERDLSVRFEANRGAVR
                     EAMKKLEQLGIANIQPGGARVAPLEEASLDVIGHMLALGDLPDSKLVIHIIEVISALV
                     RLAVESALNTATDEDIERLREHNRPLFEEDLDDLAHMEARLELMGAIMSASDNLVCQL
                     IARSLLAQLTPRTEPLKAFAQFDTEAHADNARRLDEALAKRDADALRHVFTESTALTR
                     ESVLRSFEAYDAANRAPLTGVAAS"
     gene            6270..7424
                     /locus_tag="AC16PP_14850"
     CDS             6270..7424
                     /locus_tag="AC16PP_14850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6482957.1"
                     /translation="MMKKLLVPGLVILASLFGAVTLMATAPELEPNAIEPLATTVRVV
                     EVIPQPVRLTVHSQGTVVPNMESELIPEVSGRVEWISPSLVNGGYFEQGDVLLRLEAT
                     DYRSALTRAEAHLDRAAAEFEHAQYEYQRLKSLEVRQLASKSQMENSLRAFRVAEASL
                     QEARAAMEQAMRDLERTALTAPFDGLVRREAVDVGQFVSRGSPVATVYASDQAEVRLP
                     IADRQLAFLNLPVDHRGELPAEQQPRVTLLAEYAGRSLEWQGTIVRTEAQIDTASRMV
                     HVVARISNVEQPVPLNVGLFVNAEIEGLLADNIVVLPRTALRDGDRVLVVDGGNNLRY
                     RNIEPLRLYKDEVLIKAGLAAGERVCVSPLQTAVDGMPVQPVTENAPTAG"
BASE COUNT         1383 a         2518 c         2365 g         1297 t
ORIGIN      
        1 tccaggttgc gagcgtgctc gagcattcgc tcctccagct ccgagagcag cctgccaaac
       61 gctttcggat cctctggaat ctcgatgcgc tcggccgatc cgtgtgcagg cccggcgcct
      121 ttccggcgca ccgtgccggg cgcgggagct ctggcaccct ccatcacgtc ggcgatctgc
      181 ttttcgatgg tgcgtggcgt gatgccgtga gcctcgttga acgccgtctg cttggcacgc
      241 cggcgttcgg tctctccgat ggccttgcgc atggaaccgg tttcatgatc ggcgtagaga
      301 atcgcccggc cattgacgtt gcgcgcggcg cgaccgatgg tttgaatcag cgaacgttcg
      361 gcgcgcagaa agccttcctt gtcggcgtcg agaatggcca cgagggagac ctcgggcatg
      421 tccaggccct cccgaagcag attgatgccg accagaacat cgaactcgcc gaggcgcaga
      481 tcgcggatga tttccatgcg ttccaccgta tcgatgtccg agtgcagata gcgcacccgg
      541 acgccgtgct cgtccaggta ctcggtgaga tcctctgcca ttcgcttggt cagggtggtg
      601 acgagcacgc gctccctgtt ctcaaccgtg cggcgaattt cgccgaacag gtcgtcaacc
      661 tgcgtcgacg cggggcgcac gatcacgtcc ggatccacca ggccggtcgg acgcaccacc
      721 tgctcgacga cggcgccgga ccgttccgcc tcgtaaggtc ccggggtggc ggagacgaag
      781 atggtctggg ggctcatgcg ctcccactcg tcgaatcgca gcggccggtt gtccagagcg
      841 gaaggaagcc ggaagccgta ctccaccagc gtctcctttc tggaacgatc acctttgtac
      901 atgccaccta tctggggaac ggtcacgtgg ctttcgtcga tgaccagcaa cgcgtccttg
      961 ggcaggtaat cgaacagggt cggcggcggc tcacccggct cgcggccgga caggtagcgc
     1021 gagtagttct cgataccact gcagtagccc agctcgcgga tcatctccag atcgaagcgc
     1081 gtacgctggt ccagccgctg agcctcgacc agcttgtgat gcttctgcag ataggccagc
     1141 ctatcctgca actcttcctt gatgtcttcg agcaccgcaa gaatgcgctc gcgcggcgtc
     1201 acgtagtgag tcttgggaaa gaccgtgtag cgaggcaccc ggttgacgat ctcgcccgtc
     1261 agaggatcga agacggagat gttttcgatc tcgtcgtcga agagctccac acgcacggcc
     1321 tccttctccg actccgcggg aaatatgtcc acgacgtcgc cgcgaacgcg gtaggagccc
     1381 cgttgaaaag ccatgtcgtt gcgcgtgtac tgcagctccg ccaagcgctg cagcacgtag
     1441 cgctggtcca cgcgatcccc acgatccaga tgcagaacca ttcgcaggta ggactgaggg
     1501 tcacccagcc cgtagatggc cgagaccgac gccacgatga tggtgtcgcg acgctccagc
     1561 agcgccttgg tcgccgacag ccgcatctgc tcgatgtgcg cgttgatcga cgagtccttc
     1621 tcgatgtaag tgtcggaagc cgggacgtag gcctccggct ggtaatagtc gtagtaggaa
     1681 acaaagtact ccacggcatt gttcggaaag aaatcccgaa actcgccgta gagctgtgcc
     1741 gccagagtct tgttgggagc caggacgagc gtcggccgct gaacggcctc gatgacgttg
     1801 gcgacggtgt acgtcttgcc tgacccggtt acgccgagga gcgtctggtg gctcaggccg
     1861 tcgacgagac cctcgacgag accggcgatc gccgtcggct gatcgcccgc gggctcatag
     1921 tcggaaacga cctcaaattc cgcgacggcc ttgaccattg agcgctattc cggtttgggt
     1981 tcctcttcgc cctcttcggc cgaagccggc tcgcccgctt cgtcttccgc agcaacctcg
     2041 gccgcggcgg cttcctcggc aggagcagcc gcctcggcgg gttcagactc ttccgcggcc
     2101 gactcttcgg ccccagtgac ctcttcggcg gccgcggctt ccgccgccgg ctcgccggcg
     2161 tcggcttcgg gctcttcgag cggcgcggcc ggcagggcgg ccgtaatcag ctcgtgctgg
     2221 acgaggatgg cggccttcgc gtctccattg acccaggcct tcgtggcatc cttcacggca
     2281 taacgttcgt cgcggcgctg gtaaatggtg tactcgtcgg ttttgactac gactttcact
     2341 tcaggctcca gagtgtggca agcggcgcac tctaagggat ttgaaccgca aaatcatccg
     2401 gcgcctgcag gggcagtcag gacataacct tatgtcccat tggatctcca cttgcgcgcg
     2461 gaattaagta caattccgca cctttttttg cgggcgggct ccgggagcct caaatggtcg
     2521 aggaaaaaac cagctacgtt atccgctttg cgggggactc cggtgacggc atccagcttc
     2581 agggccagca gttcaccacg gcctccgctc ttgccggcca cgacctggcc accatgcccg
     2641 actttccggc ggagatccgc gcgccggccg gtacccggta cggcgtgtcc gcgtttcaga
     2701 tccagttcgg cggcgaccgg gtcaccacgc cgggcgatgc tcccgacgtc ctcattgcct
     2761 tcaacccggc cgcgctggtg gtcaacctgt cccatctgag gccgggcgcg ctggtcgtcg
     2821 tcgaccggca ggccttcacc gaccagcgcc tgaagcgcgc ggacctgacg agcaaccccc
     2881 tcgacgacgg caccctcgat ggtttcgagc tgctcgacat cgacatctcg gatctgaccc
     2941 accaggctgt agacgctctc gggctgggcg ccaaggactc actgcgctgc aagaacttct
     3001 gggcactggg actcgtgctg tggatgttcg atcagaaacg cgacgccacg gtctcctgga
     3061 tcgaggcgaa attcgccggt aacgagcaag tgcgaagcgc caacctcgca gctctgaacg
     3121 ccggccacgc tttcggcgag acggccgagc tcggcccgaa gctgcgtcag cacgccgcgc
     3181 ccgaagcctc gctggcgtcg gccgagtacc ggaccctcac cggcgccgag gcgctggccc
     3241 tgggcctggt ggccgccggg gagctcgcgg atacggagct gatgttctgc tcctacccca
     3301 tcacgccggc ctcctcgctg ctgcaccagc tgaccgggct ggaggatgcc ggggtggcga
     3361 ccttccaggc cgaagacgag atcgcggcga tctgctcggc catcggcgcc tcgtacgccg
     3421 gcaagctggg cgtcacttcg agctccgggc cgggaatcgc gctgaagacc gaggccatgg
     3481 gcctggcggt ggccgccgag ctgccgctga tcatcgtaaa ttcccaacgg gcggggccat
     3541 ccacggggat gcccaccaaa accgagcagt ccgaccttta ccaggccgtg tacggtcgca
     3601 acggggacac gccgctgccg gtggtcgcct cccgctcccc cgcggattgc ttcgataccg
     3661 ccatcgaggc ggtgcgcatc gcgctgcggc acatgacgcc ggtgattctt ctgaccgacg
     3721 gctacatcgc caacgcctcc gagctttggc ccctgccgga cttcgatcac tacgagccga
     3781 tagaaacgca caaaccggat cccagcaccg atccacgcca ttccgttttc agccgcgatc
     3841 cgaagaccga ggcccgtctg tgggcaacgc ccggggacaa acgcttcgtc taccgggtcg
     3901 gcggcatcga gaaggacatc cacaccggca acatctccta cgaagctcag aatcatcagg
     3961 aaatgaccaa catgcgcgag gccaaggtgg ccagcgtcgc cagcttcatc cccgatcagc
     4021 aggtggattc gggaaccgat gccgggctgg ccgtagtggc ctggggatcc acgtacggaa
     4081 cgatctatca ggcagtcaga gagtgcgtcg ccgaggggct ggccgtgggt cacatacatc
     4141 tgcggcactt gaacccgctg ccgccgaacc tgccgaccct gctggcgcag ttcgaccgga
     4201 tcctcgttcc cgagctgaac aacggccagc tcgcgaccct gctgcgagac aagctggtcc
     4261 tcccggtgga gcaacacaat caggtcaacg ggcagccgtt tgccgtggcg gacgtcaagc
     4321 aggcgatcgc gcgatacgcg cccaagcgca tcaaggaggc aggttgaatc atggcggagc
     4381 tgactttcaa agactacgcg actgaccagg aagtgcgctg gtgcccggga tgcggtgact
     4441 actccatcct caaggccgtg cagcgggcgc tggctgatgc tggagccgcc aaagagcaga
     4501 cggtattcgt gtcgggcatc ggctgctcgt cacgccttcc ctactacgtg gacacctacg
     4561 gttttcacac cattcacggc agagcgcccg ccgtcgcgac cggggtgaag ctggccaacc
     4621 ccgacctcga cgtctgggtg atcacgggtg acggcgacgc cctctccatt ggcagcaatc
     4681 acctaatgca cgcgcttcgc cgcaacgtgg acctgaatat cctgctattc aacaacgaga
     4741 tctacggcct gaccaaaggg cagtactcgc caacgtcgcg catcggcacg agaagtcctt
     4801 ccagcccgaa gggatccgtg gacagccccg tgcaaccggg cttgttcgcc gtcggtgcgg
     4861 gagccacctt catcgccaga gccgtggacg tcgatcagaa ggtattgccg ggaatcctca
     4921 acgcggcgcg ggaacacaaa ggcgccgcgc tggtggagat cctccagaac tgcatcgtct
     4981 acaacaaaga cgtcttcgag gacgtggtcg ccaagaaaac ggcggcggac actcaggtcg
     5041 tcgtggagca cggccatccg ctcgtgttcg gcaaggagcg cgacaagggg ttgcgcctca
     5101 atccgcagac cctggcgctg gaagcggtga cgctcggcga gaacggcgtg agcgaggccg
     5161 atctggtggt ccacgacgag accaataccg ccctggcgca catgctgctg tccctggcac
     5221 caccgaaccc ggtggccatg ggcatcatcc accgacgtcg cgcgccggcc ttcgacgccg
     5281 cctactggtc gagcaaaccg accgggcgca gcgcccgggt cgccgagttg ctgcgccacg
     5341 gcaacgtcct ggatcaccgc cgctctcctc agggccgctg accgccgccc atccccaccg
     5401 cggccactgg ccgcggtttc gcgaacgcgc gcacatttcg ttaccaaagg taacgaaatg
     5461 ctggacatgc gaacctgatt gactaaaatg ccccgaccat catggtcaga ccaacatggt
     5521 cgcaccagtc agaacaggaa gaacatggca aagcacgagg aaatcgccga gagcctcacc
     5581 agggacattc tgagcggcca gtaccgcacc ggcgagcggc tgccctcgga acgggatctt
     5641 tccgtgcgct tcgaggccaa ccgcggcgcc gtccgggagg ccatgaagaa actggagcag
     5701 ctcggcatcg ccaacatcca gcccggcggc gcgcgggtgg cgcccctcga agaagccagc
     5761 ctggacgtga tcggccacat gctggccctg ggcgaccttc ctgacagcaa gctggtgatt
     5821 cacatcattg aagtcatttc cgcccttgtc aggcttgcgg tggagtcagc gctgaatacg
     5881 gccaccgacg aagacatcga gcgcctgcgc gaacacaacc gaccgctctt cgaggaggat
     5941 ctggacgacc tcgcccacat ggaggctcgc ctggagctga tgggcgcgat catgagcgcc
     6001 agcgataatc tcgtatgcca gctcatcgcc cgcagcctgc tggcacagct cacgccgcga
     6061 accgagccgc ttaaggcatt cgcccagttc gacacggagg cccatgcgga caacgcccgg
     6121 cgactagacg aagccctggc caaacgtgac gccgacgctc tgcgccacgt gttcaccgag
     6181 agcactgcgc tcacgcgcga atccgttcta cggtccttcg aagcctacga cgcggccaac
     6241 cgcgcaccgc tcacgggggt agccgcgtca tgatgaagaa gctgctcgtt ccgggactcg
     6301 tcatcctggc atccctcttc ggagcggtaa cgctgatggc aacggccccc gagctggagc
     6361 ccaacgccat agagcccctg gccaccacgg ttcgagtggt ggaggtgatt ccccagccgg
     6421 tccgcctgac cgtgcactcc cagggtaccg tggtaccgaa catggagagc gagctgatcc
     6481 cggaagtgtc cggacgcgtc gagtggatct cgccgtcact ggtcaatgga ggttacttcg
     6541 agcagggcga cgtgcttctg cgcctggaag ccaccgacta ccgctcggcg ctgacgaggg
     6601 cggaagccca tctggacagg gccgcggcgg agttcgagca cgcgcagtac gagtaccagc
     6661 ggctgaagag cctggaggta cgccagctgg cgagcaaatc ccagatggag aactccctgc
     6721 gcgcgttcag ggttgccgaa gcatccctcc aggaggcgcg cgccgccatg gagcaggcga
     6781 tgcgcgacct cgaacgaacc gccctcaccg cccccttcga cggcctggtt agacgcgaag
     6841 ccgtggacgt cgggcaattc gtcagccgcg gctctccggt ggcgaccgtt tatgccagcg
     6901 accaggcgga ggtccggctg cccatcgccg accgccagct ggcgttcctc aacctgcccg
     6961 tggatcaccg cggcgagctt cccgccgagc agcagccgcg ggttacgctg cttgccgagt
     7021 acgccggccg cagcctggaa tggcagggaa ccatcgttcg aaccgaagcc cagatcgaca
     7081 cggcgagccg catggttcac gtcgtcgccc ggatttccaa cgtggagcag ccggtgccgc
     7141 tcaacgtggg cttgttcgtc aacgcggaga tcgaggggct gctggccgat aacatcgtcg
     7201 ttttgccgag aacggcgctg cgcgacggcg atcgagtgct ggtggtggat ggcggaaaca
     7261 acctgcgcta tcggaatatc gagcccctgc gcctgtacaa ggacgaggtg ctgatcaagg
     7321 cggggttggc cgccggcgag cgcgtctgcg tatctcccct gcagaccgcc gtcgatggca
     7381 tgccggtcca acccgtcaca gagaacgccc cgacagccgg ctgagcccat gtacaccgcg
     7441 atttcctggt tcacgaggaa ccccgttgcc gcgaacctgc tgatgttcgt cctgctgatc
     7501 ggcgggtgcc tggcgctttt caacgtccat caggaagagt tccccagcat ggacgttcgc
     7561 gtc
//
LOCUS       DBNHKV010000451         7378 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_136796, whole genome shotgun sequence.
ACCESSION   DBNHKV010000451 DBNHKV010000000
VERSION     DBNHKV010000451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 7378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7378
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_136796"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="AC16PP_14855"
     CDS             complement(<1..534)
                     /locus_tag="AC16PP_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014336251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6482958.1"
                     /translation="MPLIILGLSGALNHDPSAALLVDGEIVAAVEEERFLRDKHAKNR
                     MPLESARYCLRAGGISPRDVDVVAFPYAPIALSSAARWHYARRHWYAPDRALDALFNG
                     NRRYRRNRRRVLELGDQLGIDWSRTRFEPVEHHLAHASSAYHLSGFTEKTAIMGVDGK
                     GEYATTFFGYGENGRIHR"
     gene            complement(559..1974)
                     /locus_tag="AC16PP_14860"
     CDS             complement(559..1974)
                     /locus_tag="AC16PP_14860"
                     /inference="COORDINATES: protein motif:HMM:NF018047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HIR6482959.1"
                     /translation="MTATGRPLSLAELQAQGRDLPTPFILEGSLNPAPDDGPIECLEV
                     LRVLPGRRIVMRARLGARQCALKLFVGDRAALYARRERAGLELLAASGVDTPELIADL
                     HLPGGGEGILVDWLAGSRPVDEEDVQGLADVAAVLARLHANGAIQHDAHLNNYLRDAD
                     GMLHAIDGDGVRRPARLRRRQELANLALLLAQLPPPDATRLPVVAEAYWRARWGSDPG
                     PDELAALQRRVHAQRAGRMRRYLRKTLRDCSEFHGERKKGRFVVCARNAWTAQMAEFV
                     DRPEDHLVAGTVLKAGNSATVMRLRIGETAYVVKRYNVKGFFHGLRRALRPTPRYRVA
                     WLSGHRLLRLGIATPRPVALLEARQGLATVAYLVMEDLGAARDLAQAVAEEGYTAGVG
                     RAVAGLFRGLRDAGLVHGDTKASNFLVRDASVYLIDLDAMKPGSQTRSRDVARFLANW
                     DDAPQVCESFRGALQEAGVPL"
     gene            complement(1971..3026)
                     /gene="waaC"
                     /locus_tag="AC16PP_14865"
     CDS             complement(1971..3026)
                     /gene="waaC"
                     /locus_tag="AC16PP_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008016956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HIR6482960.1"
                     /translation="MHVLLIKMSSMGDVVHALPAVTDAASHGVTFDWVVEEAFADIPA
                     LHPAVGQVMPIAWRRWRSSLRSSGGELARFWEQLRRRRYDLVLDSQGLVKSGVVAALA
                     RGRTKAGFSHASARERAAALAYRRGVRVARNQHAIDRQRELFAAVFGYCVPAGLDYGI
                     ARAAHQASKTNHCVLLHGTTWESKHYPEHLWREVARRARAAGLDVALPWGSEAERARA
                     EAIATTNADVLPALNLAELTTRIASARLVVGVDSGLAHLSAALDVPTVVIYGSTDSAL
                     TGCRGRRVSNLQAQFPCAPCLRRACGYHGPEEILHQAPVRPACYSRVTPDAVWDAAAR
                     AMADGGRETAPNPGSDL"
     gene            complement(3026..4030)
                     /gene="waaF"
                     /locus_tag="AC16PP_14870"
     CDS             complement(3026..4030)
                     /gene="waaF"
                     /locus_tag="AC16PP_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017336199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HIR6482961.1"
                     /translation="MPNSGTRILIVAPAWVGDMVMAHSLVQVLIARHGDPGIDMLAPR
                     ATAGLGARMPGVRNVHVLDVEHGELALGARRSSGLTLKDQYDQAIVLPNSFKSALAPA
                     WARIPVRTGWSGEARVGVLNDRRRLNPERYPRMVDRFAALGLDPDAPLPDDIPGPVLE
                     PDAAAAERLLASRRLSVDGGVTALCPGAEFGPAKRWPSVHFAQVASYAAAQGHQVWLV
                     GSPKDAGVCSEVEAQSPAGVVNLAGRTTLLEAVDLLSRADRVVSNDSGLMHVASALGR
                     PVVGVFGSTSTTFTPPLGERATTVSLGIECSPCFQRECPLGHLRCLNDLPPQRVIELL
                     "
     gene            complement(4020..4961)
                     /locus_tag="AC16PP_14875"
     CDS             complement(4020..4961)
                     /locus_tag="AC16PP_14875"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR6482962.1"
                     /translation="MTEPTFSDRDGWIWHNGELVPWRDAKVHVLTHTLHYGVGVFEGV
                     RAYQTPSGPAIFRLHEHTRRLFNSAHILGLKIPFSVDEVNAAQVDVFRANALNEGYLR
                     PMVYLGSEAMGLRAKDLSVNLCVAAWAWPDYMDPDSREKGINVRTSSFTRHHVNITMC
                     KAKANGNYINSILALHEALDSGCDEALLLDNEGYVAEGSGENVFIVKDGEVHTPELTS
                     CLDGITRNTIFHLAAEMGIAIRERRITRDEVYIADEAFFTGTAAEVLPIRELDGRMIG
                     SGKRGPVTERFQSAYFDQVRGKRSAFPEWHTPVSGAQ"
     gene            complement(4972..>7378)
                     /gene="glnE"
                     /locus_tag="AC16PP_14880"
     CDS             complement(4972..>7378)
                     /gene="glnE"
                     /locus_tag="AC16PP_14880"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HIR6482963.1"
                     /translation="TCATLSMLADTMLDLALERLYQWATDRQGVPMGESSRSPQRLVV
                     LALGKLGARELNLSSDIDLVFAYPEPGATTSGTTNQQFFLRLGQQLIQALDAVTADGF
                     AFRVDMRLRPFGASGPLVMHFEGMEDYFAAHGRDWERYAWIKARACAGDVASGEALLQ
                     SLRPFVYRRYLDFGAVDALRDMKRRLYSERNDPNDVKLGPGGIRDVEFVVQLQQMVWG
                     GRQPELQEANLLRVLPRLASQGHLSDADAAALAEGYRFLRDTEHSIQAEGDLQTQSLP
                     ASEDSRQRLASSRGFADYGAFESALNGHRIAVEAVFRNAVGELPEEDTPGQRLWSSPD
                     DAALLGELGFARAEAAGKLLTSLMEARDRPSQSAESRARLDRLMPGLLDRVSLTEDPD
                     LSLARVVPVLRSVLRRSAYLSLLRENPHTLAHFVDLTAKSLWLAEQLARFPAFFDALL
                     DERQLAALPDKAALQAELHQQVEAAPADDFERRLDVLREFKEHHVFNVSLAEVRGTLP
                     LMKASDYLTFLAEAVLGESLDLAWNENADRFPEFAEPRPFIIVGYGKLGGIELGPGSD
                     LDLVFIHDLPVGASQFLHRMVRRLLHILTVPTYSGTLYEIDMRLRPSGSSGTMVSSLE
                     AFAEYQRSQAWMWEHQALVRARAVAGDPALAQRFEAVRRELLCEPRDRGPLREEVLKM
                     RARMAEHHGDAEDLKRGSGGIVDIEFMVQYLVLAWAHEHPALAEFSDNVRILETAERL
                     DLLPANMARNLREAYVGLRSEWHRSVLDLPDSERAARTLMAHRSDVRDAWREVFGIDP
                     ENE"
BASE COUNT         1260 a         2521 c         2425 g         1172 t
ORIGIN      
        1 ccggtgaata cggccgttct cgccgtagcc gaagaacgtg gtggcgtact cacccttgcc
       61 atccaccccc atgatggccg tcttctccgt aaagccgctc aggtggtagg cgctggaggc
      121 gtgggcaaga tgatgctcca cgggctcgaa tcgggtccgg ctccagtcga tgccgagctg
      181 gtcgccgagc tctaaaacgc gccgccggtt tcggcgatac cgacggttgc cgttgaaaag
      241 cgcatcgagc gcccgatcgg gcgcgtacca gtggcgtctg gcgtagtgcc agcgcgcagc
      301 gctggacagc gcgatcggcg cgtagggaaa cgcgaccacg tccacgtccc ttggcgaaat
      361 cccaccggcg cggagacagt agcgggccga ttccaacggc atgcggttct ttgcgtgctt
      421 gtcgcgcaga aaccgttcct cttcaaccgc cgcgacgatc tccccgtcga caagcaacgc
      481 ggccgaaggg tcgtggttga gcgctccgga aaggccgagg atgatcaacg gcaaacctgg
      541 gatcggaaag tgcgcgtact atagcggcac gccggcctcc tgcaaggcgc cgcgaaaaga
      601 ctcgcacacc tgtggcgcat cgtcccagtt cgccaggaaa cgggcgacgt cgcgggaccg
      661 cgtctggctg cccggcttca tggcatccag gtcgatgagg tagacgcttg cgtcgcgcac
      721 caggaagttg ctggctttcg tatccccgtg caccaggccc gcgtcgcgca gcccgcggaa
      781 caacccggca accgcacgtc cgacacccgc ggtgtatcct tcctcggcga cggcctgcgc
      841 cagatcccgc gccgcaccga gatcctccat gacaagatag gccacggtag caagcccctg
      901 gcgcgcttcc agcagagcga ccggccgggg cgtggcaata cccaggcgaa gcaggcgatg
      961 accgctcaac cacgccacgc gatagcgcgg cgtggggcgc agcgcccgcc gcagcccgtg
     1021 aaagaagcct ttgacgttgt agcgcttgac cacgtacgcc gtttcgccga ttcgcaggcg
     1081 catcaccgtg gcgctgttgc ctgccttgag cacggtgccg gccaccaggt ggtcttccgg
     1141 acggtcgacg aactcggcca tttgcgcggt ccaggcattt cgggcacaga ccacgaagcg
     1201 gccctttttc cgctcgccgt gaaactcgct gcaatcccgc agcgtcttcc ggaggtaacg
     1261 gcgcatcctg ccggctcgct gagcgtgcac ccgtcgctgc agcgcagcca gctcgtccgg
     1321 gccggggtcc gatccccagc gcgcacgcca gtaagcttcc gccaccaccg ggaggcgagt
     1381 cgcatccggc ggcggcagct gggccagcag cagggcgagg ttggcgagtt cctgtctgcg
     1441 ccgaagacgt gcaggccggc gaacgccgtc gccgtcgatg gcatgcagca tgccgtctgc
     1501 atcgcggagg tagttgttca gatgagcgtc gtgctggatg gcaccgttgg cgtgcaggcg
     1561 tgcaagcacg gccgcaacat ccgccaggcc ttggacatct tcctcgtcca ccggccggct
     1621 gcccgccagc caatccacca ggatgccctc cccgccgccg gggagatgga ggtcggcaat
     1681 cagctccggc gtgtccaccc cgctggccgc cagcagttcc aggccggctc gctcccgcct
     1741 ggcgtaaagg gcagcccggt ccccaacaaa gagcttgagc gcgcattgac gagcccccag
     1801 ccgcgctcgc atgacgattc gccggcccgg gagcacgcga aggacctcga gacactcgat
     1861 cggcccatcg tcaggggccg gattgaggga tccttccaga atgaacggcg tcggcagatc
     1921 gcgtccctgc gcctgcagct ccgccaaaga aagcggccgg cccgtggccg tcacaggtcg
     1981 gaccccgggt ttggggcggt ctcccgaccc ccatccgcca tggctcgggc tgcggcatcc
     2041 cacaccgcat cgggcgtaac gcggctgtag cacgcgggcc gcaccggcgc ctgatgcaga
     2101 atttcctccg ggccgtgata gccgcaagct ctgcgcaggc agggcgcgca cggaaactgc
     2161 gcctgcagat tgctcaccct gcggccgcgg catcccgtga gtgcgctgtc cgtgctgccg
     2221 tagatcacca ccgtaggcac gtcgagggcc gcgctgaggt gagcgagtcc cgagtccacg
     2281 cccacgacga gccgagccga ggcgattcgc gtggtcagct cggcaaggtt cagggccggc
     2341 aggacgtcgg cgttcgtcgt cgcgatcgct tccgcccgcg cacgctccgc ctcggagccc
     2401 cagggcagag ctacgtccag cccggccgcg cgagcgcgcc gggcgacttc ccgccaaaga
     2461 tgctccgggt agtgcttgct ctcccaggtg gttccgtgca gcagcacgca atggttggtc
     2521 ttgctggcct gatgcgccgc ccgcgcgatt ccgtagtcga gcccggcagg gacgcaatag
     2581 ccgaacaccg cggcgaagag ctcgcgttgg cggtcgatgg catgctggtt gcgcgccacc
     2641 cgaaccccgc gccgataagc cagggcggcg gccctttccc gagccgaggc gtgagagaac
     2701 ccggccttgg ttcttcctct tgccagcgcc gccaccacgc cgcttttgac gagcccctga
     2761 gaatcgagga cgaggtcgta gcgccggcga cggagctgct cccagaaccg ggccagctcc
     2821 cccccggacg agcgcagcga ggagcgccat cgccgccagg caatgggcat cacctggccg
     2881 actgccgggt gcagcgcggg tatgtccgca aacgcctcct caactaccca gtcgaaagtc
     2941 accccatggc tcgcggcgtc cgtcaccgcg ggcagagcat ggaccacgtc ccccatcgac
     3001 gacatcttga tcagcaggac gtgcactaga gcagctcgat gactcgttga ggcggcaggt
     3061 cgttgaggca gcgcagatgc cccagcgggc attcgcgttg aaagcacggg ctgcactcga
     3121 tacccaggct caccgtcgtc gctcgctcgc cgagcggcgg cgtgaaggtc gtggaggttg
     3181 atccgaagac gccaaccaca ggccggccga gcgccgaggc aacgtgcatg agacccgaat
     3241 cgttgctgac gacccggtcg gccctggaga gcaggtcaac ggcttccagc agcgtggtgc
     3301 gccccgcgag gttgaccacg ccggccggcg actgcgcctc cacctccgag cataccccgg
     3361 catctttcgg cgaccccacg agccatacct gatgtccttg agcggcggcg tagctggcga
     3421 cctgggcaaa gtgaacggac ggccagcgct tggccgggcc gaactcggcc cccgggcaca
     3481 gggcggtcac gcccccgtcc accgagagcc ggcgggaggc gagcaggcgc tccgcggcag
     3541 cggcatcggg ctcgaggacg ggaccgggaa tatcgtccgg cagcggcgcg tcgggatcga
     3601 gtcccagagc cgcgaaccgg tcgaccattc tcgggtagcg ctccgggttc aggcggcgcc
     3661 ggtcgttgag caccccgacc cgtgcttcgc cgctccagcc ggtgcgaacg ggaatcctgg
     3721 cccacgcggg tgcgagcgcc gacttgaagc tgttcggcag cacgatggcc tggtcgtact
     3781 gatccttgag ggtcagcccc gagctgcgcc tggcgcccag ggcgagctca ccgtgctcca
     3841 cgtcgagcac gtgaacgttc ctcacgcccg gcatgcgggc tccgagcccg gccgtggcgc
     3901 gaggggccag catatcgatc ccggggtcgc cgtgccgcgc gatgaggacc tgcacgaggc
     3961 tgtgcgccat gaccatgtcg ccaacccaag cgggggcgac gatcaggatg cgggtgccgc
     4021 tattgggcac cgcttacggg cgtgtgccac tccgggaagg cgctgcgttt gccccgcacc
     4081 tggtcgaagt aggccgactg gaaccgctcc gtgacgggcc cgcgtttgcc cgagccgatc
     4141 attcgaccat cgagctcgcg aatcggcagc acctcggcgg ccgtccccgt gaagaacgcc
     4201 tcgtcggcga tgtacacctc gtctctggtg atgcgccgct ccctgatggc gatgcccatc
     4261 tccgcggcca gatggaagat cgtatttcgc gtgatgccgt cgagacagga ggtaagctcc
     4321 ggcgtgtgca cctcgccatc cttgacgatg aagacgttct cgccgctgcc ctcggcgacg
     4381 tatccctcgt tgtcgagcag cagcgcctcg tcgcatccgc tgtccagcgc ctcatgaaga
     4441 gcgagaatgg agttgatgta gttgccgttg gctttcgcct tgcacatggt gatgttcacg
     4501 tggtggcggg tgaaggacga cgtccgcacg ttgatcccct tctcgcggct gtcggggtcc
     4561 atgtaatccg gccacgccca ggcggcgaca cagagattta ccgaaagatc cttggcccgc
     4621 aggcccatgg cctctgaacc caggtacacc atgggccgca ggtagccttc gttcagagcg
     4681 ttggcacgga aaacgtcgac ctgggctgcg ttgacctcgt cgacgctgaa ggggattttg
     4741 aggccgagga tgtgcgccga gttgaaaagc cggcgagtat gctcgtgaag cctgaaaata
     4801 gcggggcccg agggggtctg atacgcccgc acgccttcga acactcccac gccgtagtgc
     4861 agcgtgtgag tcagcacgtg gactttcgcg tcacgccagg gcaccagttc cccgttgtgc
     4921 cagatccagc cgtctcggtc cgaaaatgtc ggttctgtca tgattccgcg cttactcgtt
     4981 ttccgggtcg ataccaaaca cctcgcgcca cgcatccctc acgtcggacc tgtgggccat
     5041 cagggtccgg gcagcccgct cgctgtccgg caggtcaagc accgaccggt gccactcaga
     5101 gcgcagaccc acgtaagcct cccggaggtt ccgggccatg ttcgcgggaa gcagatccag
     5161 ccgctcagcc gtctctagaa tgcgcacgtt gtctgagaat tcagcgagcg cagggtgttc
     5221 gtgcgcccag gccaggacca ggtattgcac cataaattcg atatcgacga taccgccgga
     5281 gcctcgcttg aggtcttccg cgtcaccatg gtgctcggcc atgcgggctc gcatcttcag
     5341 cacttcctcg cgcagcggcc cgcggtcccg gggctcgcac agaagttccc gtcgaaccgc
     5401 ctcgaagcgc tgggcgagag ccggatcgcc agccactgcg cgcgcccgaa ccagcgcctg
     5461 atgctcccac atccaggcct gggatcgctg gtactcggcg aaagcctcca ggctcgaaac
     5521 catggttccc gaactgcccg aagggcgcag gcgcatgtcg atctcgtaaa gcgtcccgga
     5581 ataggtggga acggtgagga tgtgaagcag gcggcggacc atccggtgca ggaactgcga
     5641 ggcgccgacg ggcaggtcgt gaatgaagac caggtccagg tcgcttcccg gcccgagctc
     5701 gataccgccg agcttgccgt aaccgacgat gatgaaaggt cgaggctcgg cgaactcggg
     5761 aaagcggtcg gcgttttcgt tccaggccag gtccagcgac tcgccgagca cggcctcggc
     5821 cagaaacgtg aggtagtcgc tcgccttcat caggggcagc gttccacgaa cctccgccag
     5881 ggagacattg aacacgtgat gctccttgaa ctccctgagc acgtcgagcc gacgttcgaa
     5941 gtcatccgcc ggcgccgcct cgacctgctg gtgcagctcg gcctgaaggg cggccttgtc
     6001 cggaagcgca gcgagctggc gttcgtccag cagcgcgtcg aagaacgctg gaaagcgggc
     6061 gagctgctcg gccaaccaca agcttttggc cgtcaggtcg acgaagtgag caagggtgtg
     6121 ggggttttcg cggagcagcg acagataggc cgaccgccgc agcacggatc gcaacacggg
     6181 caccacgcgg gcaagcgaga gatccggatc ctccgtaagg ctcacccggt cgagaaggcc
     6241 gggcatgagc cggtccagcc gggctctgct ttcggcgctc tgggagggcc gatcgcgcgc
     6301 ttccatcagg ctggtcagca gcttgcccgc cgcctcggct cgcgcgaaac ccaattcgcc
     6361 gagcagcgcg gcgtcatccg ggcttgacca gaggcgctgg ccgggcgtgt cttcctccgg
     6421 cagctccccc accgcgttcc tgaagacggc ttcaaccgcg atccggtgcc cgttcagcgc
     6481 gctttcgaac gcgccgtagt cggcaaagcc cctggagctg gcaagccgct ggcggctgtc
     6541 ttcggacgcc ggcagcgact gggtctgaag gtcgccctcg gcctggatgc tgtgctcggt
     6601 gtcacgcagg aatcgatagc cttcggccag cgcggccgcg tccgcgtcgc tcagatggcc
     6661 ctgggaggcg agccgcggca ggacacgcag caggttggct tcctgcagct ccggctgcct
     6721 cccgccccag accatctgct gcagctgaac gacgaactcc acatccctga tcccgccggg
     6781 cccgagcttg acgtcattgg gatcgttacg ctcggagtag agcctgcgct tcatgtcgcg
     6841 cagcgcgtct actgccccga aatcgagata ccgcctgtac acgaagggcc gcagggactg
     6901 aagcagggcc tctcccgacg ctacgtcccc tgcgcaggct cgcgccttga tccaggcata
     6961 gcgctcccag tccctgccat gagcggcgaa gtagtcctcc atgccctcga agtgcatgac
     7021 gaggggcccg ctggcgccaa atggccggag cctcatgtcg acgcgaaagg caaagccgtc
     7081 cgccgtcacc gcatccagcg cctgaatgag ctgctgaccc aggcggagga agaactgctg
     7141 gttggtggtc ccgctcgtcg tggcgccggg ctccgggtag gcgaatacca gatcgatatc
     7201 cgacgagaga ttcagctcac gggcaccgag tttccccaac gccagcacca ccaggcgctg
     7261 gggcgatcgg ctcgactccc ccatcggcac gccttgcctg tccgtggccc actggtaaag
     7321 acgctccagg gcgagatcca gcatggtgtc tgccagcatg gagagggtgg cgcaggtt
//
LOCUS       DBNHKV010000452         6637 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_630288, whole genome shotgun sequence.
ACCESSION   DBNHKV010000452 DBNHKV010000000
VERSION     DBNHKV010000452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6637)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_630288"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3274
                     /gene="rpoC"
                     /locus_tag="AC16PP_14885"
     CDS             <1..3274
                     /gene="rpoC"
                     /locus_tag="AC16PP_14885"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR6482964.1"
                     /translation="ADMIKGKQGRFRQNLLGKRVDYSGRSVIVVGPTLRLHQCGLPKK
                     MALELFKPFIFGKLEARGLATTIKAAKKMVERETPEVWDILAEVIREHPVLLNRAPTL
                     HRLGIQAFEPVLIEGKAIQLHPLVCTAYNADFDGDQMAVHVPLTLEAQLESRALMMST
                     NNILSPASGEPIIVPSQDVVLGLYYMTREMVNVPGEGMAFSDIEEVHRAYYAGRVDLH
                     ARIKVRIEEYVQRDDGSYEAVTSLAETTVGRALFYEIVPKALPFELVNKPLDKKAIST
                     LINVCYRNVGLKDTVIFADQLMYTGFAHSTSSGASIGVDDFVIPEAKTKIIEEAEAEV
                     AEIESQYASGLVTQGEKYNKVVDIWSRANDLVARSMMDGISKETVVGVVRDEHGKVVR
                     SGGKPKTEEVEQDSFNSVFIYADSGARGSPAQIRQLAGMRGLMTRPDGSIIENAITAN
                     FREGLSVNEYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDVVITEVDCGTDKGL
                     RAAAIIEGGDVVEPLGDRVLGRVVAQDVFDVDAKTLIIPGGTMLDEAWVERLDILGID
                     EILVRSAITCSTRHGVCGACYGRDLARGHQVNVGEAVGVIAAQSIGEPGTQLTMRTFH
                     IGGAASRATAIDNIQVKHGGSVRLHNLKTVERQSGELVAVSRSGEIAVADEHGRERER
                     YKLPYGAVISVADSDSVEAGQVIAQWDPHTHPIVTEVAGTVQFQDMEEGLSINRQTDE
                     LTGLTNITVLDPKDRPSSGKDLRPAVSLVDGKGKEVVLPGSDQPAHYFLPSDAILNLE
                     NKDKIGVGDVIARIPQEGSKTRDITGGLPRVADLFEARKPKEPAILAEQTGTVSFGKE
                     TKGKRRLVVTPADGDPVETLIPKWRNIGVFEGESVERGEVVCDGPPSPHDILRLKGVA
                     ELAKYIINEIQEVYRLQGVTINDKHIEVIVRQMLRKAEIVDPGESELIRGEQLEYNRV
                     TEVNEALEAEGKELAKFERLLLGITKASLATESFISAASFQETTRVLTEAAVTGKQDH
                     LRGLKENVVVGRLIPAGTGLTYHSDRKRKREEELAARLSREQGVTADDIEQALSEALS
                     SE"
     gene            3430..3801
                     /gene="rpsL"
                     /locus_tag="AC16PP_14890"
     CDS             3430..3801
                     /gene="rpsL"
                     /locus_tag="AC16PP_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR6482965.1"
                     /translation="MATISQLVRKPRKRKVDKNIVPALQGSPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGYEVTSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGTL
                     DTSGVSDRKQRRSKYGAKRPK"
     gene            3873..4343
                     /gene="rpsG"
                     /locus_tag="AC16PP_14895"
     CDS             3873..4343
                     /gene="rpsG"
                     /locus_tag="AC16PP_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR6482966.1"
                     /translation="MPRRRIVAKREILPDPKYNNQTVAKFINHVMVSGKKAVAERIVY
                     GALSRIEERDNPDPVEIFEKALEAVAPFVEVKSRRVGGATYQVPVEVRASRREALAMR
                     WLVDSARKRGEKSMAARLAGELMDAADGRGSAVRKREDTHRMAEANRAFSHYRY"
     gene            4387..6486
                     /gene="fusA"
                     /locus_tag="AC16PP_14900"
     CDS             4387..6486
                     /gene="fusA"
                     /locus_tag="AC16PP_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR6482967.1"
                     /translation="MARTTPISRYRNIGIVAHVDAGKTTTTERVLFYTGLSHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTCFWRGMNQQFDEHRINIIDTPGHVDFTIEVERS
                     LRVLDGAVVVFCATSGVEPQSETVWRQANRYEVPRIVFVNKMDRAGADFLRVVDQIKN
                     RLGAAPVPIQLSIGAEEQFKGVVDLIRMKAIIWNEDDQGLTYDLADIPEELMADAEHW
                     REQMVENAAEANEELMEKYLEEGELSAEDIKEGLRIRTLANEIVPALCGSAFKNKGVQ
                     AVLDAVVEYLPAPTEVKAIEGLLQDEETVESRQADDDAPFAALAFKIATDPFVGTLTF
                     FRVYSGVLSSGDQVYNTVKSKRERVGRMVQMHSNNRNEIKEVRAGDIAAAIGLKDVST
                     GDTLCAQDSLITLERMEFPEPVISVAVEPKSVPDQEKMGIALGKLAQEDPSFRVETDE
                     ESGQTIISGMGELHLDIIVDRMRREFGVEANIGKPQVAYRETIRAPIEQEGKFVRQSG
                     GRGQYGHVWLRIEPYEDEEGLATYEFVDEIVGGVVPREYIPAVDKGIREQMQNGVIAG
                     YPLIGVRAILYDGSFHEVDSSEMAFKIAGSMALKEGAQKASPVLLEPIMNVEVVTPED
                     YMGDVVGDLNRRRGIIAGMDENPAGKIIRADVPLSEMFGYSTDLRSATQGRATYTMEF
                     AKYAQVPESIAEGIINR"
     gene            6553..>6637
                     /locus_tag="AC16PP_14905"
     CDS             6553..>6637
                     /locus_tag="AC16PP_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR6482968.1"
                     /translation="MSKAKFERTKPHVNVGTIGHVDHGKTTL"
BASE COUNT         1385 a         1949 c         2162 g         1141 t
ORIGIN      
        1 tgccgacatg atcaagggca agcagggccg gttccgacag aacctgcttg gcaaacgggt
       61 agattactcg ggacgttcgg tgatcgtggt gggtccgacc ctgcggctgc atcagtgcgg
      121 gctgcccaag aagatggccc tggagctctt caagccgttc attttcggca agctggaagc
      181 ccggggtctt gccacgacca tcaaggctgc caagaagatg gtcgagcggg agacgccgga
      241 ggtatgggat atcctggctg aagtcatccg cgagcatccg gtgctgctga accgggcccc
      301 cacgctccac cgtctgggca ttcaggcgtt cgagccggtg ctcatcgaag gcaaggccat
      361 tcagctgcac ccgctcgtct gcacggctta caacgccgac ttcgacgggg accagatggc
      421 cgttcacgtg ccgctgacgc tggaagctca gctcgaaagc cgtgctctga tgatgtccac
      481 caataacatc ctctctccag cgagcggcga gccgatcatc gtgccctctc aggacgtggt
      541 gctcgggctc tattacatga cccgggagat ggtgaacgtc ccgggcgaag gcatggcgtt
      601 ctcggacatc gaggaggtgc atcgtgccta ttacgccggc cgggtggatc tgcatgccag
      661 aatcaaggtg cggatagaag agtacgtcca acgggatgat ggcagctacg aggccgtcac
      721 gagcctcgcg gagaccaccg tcggccgggc gctgttctac gagatcgttc ccaaggcgct
      781 gcccttcgag ctggtcaaca agccgctcga caagaaggcc atatccacgc tgatcaatgt
      841 ctgctaccgc aacgtgggcc tgaaggacac ggtcatcttt gcggaccagc tcatgtacac
      901 cggctttgcg cactcgacgt cctcgggcgc gtccatcggc gtcgacgatt tcgtcatacc
      961 ggaggccaag acgaagatca ttgaagaggc cgaagccgaa gttgcggaga tcgagtcgca
     1021 gtacgcttcg ggtctggtga cccagggcga gaagtacaac aaggtcgtag acatctggtc
     1081 gagagccaac gatctggtgg cgcgctccat gatggacggc atctccaagg aaaccgtcgt
     1141 cggcgtcgtg cgcgacgagc acggcaaggt ggtgaggtcc ggcggtaagc cgaagacgga
     1201 ggaagtggag caggactcgt tcaactccgt cttcatctac gccgactccg gcgcccgtgg
     1261 ttctcccgcg cagatccgtc agcttgccgg catgcgcgga ctcatgaccc gtcctgacgg
     1321 cagcatcatc gagaatgcga tcaccgcgaa cttccgggaa gggctttcgg tgaacgagta
     1381 cttcatctcg acacacgggg cccgcaaggg tcttgcggac accgccttga agaccgctaa
     1441 ctcagggtat ctcacccgcc ggctggtcga cgttgctcag gacgtagtca tcaccgaggt
     1501 cgactgcgga accgacaaag ggctgagggc cgcagccatc atcgagggtg gtgacgtcgt
     1561 cgagcccctg ggcgaccggg tcctcggtcg ggtagtggct caggacgttt tcgatgtgga
     1621 cgcaaagacc ctgatcattc ccggaggcac catgctcgat gaggcatggg tggaacgcct
     1681 ggacatactg gggatcgacg agatcctcgt gcgctccgcc atcacctgct ctacgcggca
     1741 cggggtgtgc ggcgcatgct acggcaggga ccttgcccgc ggtcaccagg tgaacgttgg
     1801 cgaggctgtg ggtgtcatcg ccgctcagtc catcggcgag ccgggcaccc agttgaccat
     1861 gcggacgttc catatcggcg gagccgcatc ccgcgccacc gccatcgaca acatccaggt
     1921 caagcacggg ggttccgttc ggctgcacaa cctgaagacc gtggagaggc aaagcggcga
     1981 gctggttgcg gtttcccggt ccggcgagat tgccgttgcc gacgagcatg gccgtgagcg
     2041 tgaacgttac aagctcccct atggggccgt gatctccgtg gccgacagcg acagcgtcga
     2101 ggccgggcag gtgatcgccc agtgggatcc tcatactcac cccatcgtca ccgaggtggc
     2161 cggcacggtt cagttccagg acatggaaga aggtctgtcc atcaaccgcc agaccgacga
     2221 gctgaccggc ctgaccaaca tcaccgtgct cgatcccaag gatcggccca gctccggcaa
     2281 ggatctgcgg cctgcggtga gcctcgtcga cggcaaaggc aaagaggtgg ttctgccggg
     2341 aagcgaccag cctgcgcatt attttctgcc tagcgacgcc atcctcaacc tggagaacaa
     2401 ggacaagatc ggggtgggcg atgtcatcgc ccgtattcct caggaagggt ccaaaacccg
     2461 ggacatcacg ggtggcttgc cgagggttgc cgatctgttc gaggcccgca agcccaagga
     2521 accggcgatc ctcgccgagc agacgggtac cgtcagcttc ggcaaggaga ccaagggcaa
     2581 gcgacgcctg gtggtcacgc ctgcagacgg cgaccccgtc gagacgctga ttccgaagtg
     2641 gcgcaacatc ggtgtgttcg agggtgagag cgtcgagcgc ggggaggtgg tctgcgatgg
     2701 cccaccaagc ccccacgaca ttctccgtct gaagggcgtc gccgagctgg ccaagtacat
     2761 catcaacgag atccaggagg tctatcggct tcagggtgtg accatcaatg acaagcacat
     2821 tgaggtcatc gtgcggcaga tgctgcgcaa ggcggagatc gttgatccgg gcgaatctga
     2881 gctcatccgc ggcgagcagc tggagtacaa ccgggtgacg gaggtcaacg aggcgctgga
     2941 agccgaaggc aaggagctgg cgaagttcga gcggctgctg ctcggcatca cgaaagcgtc
     3001 tctggcaacg gagtcgttca tctcggctgc ctccttccag gagacgaccc gggtgcttac
     3061 cgaggccgca gtgaccggaa agcaggatca ccttcgcggc ctgaaggaaa acgtcgtcgt
     3121 cggacggctg atcccggccg gaacgggcct gacctatcac agcgaccgca agcgcaagcg
     3181 cgaagaagag ctggcggccc gtctgtcgag ggagcagggg gtgacggcgg acgatatcga
     3241 gcaggcactt tcggaagcct tgagcagcga gtagccctgc aacaggagcg gccccaggtg
     3301 tggtcgcggg ggcggcggca atcgctggat tgccgccgtt gctaaccgca ggacgtgtga
     3361 ttacaatcgc gccccccgga gccagccctg tcggcgggtt gcgggaaaag agtcagtaac
     3421 ggagcaagaa tggcgacgat cagccagttg gtacgaaagc ctcgaaagcg aaaagtggac
     3481 aagaacatcg tccccgcttt gcagggctca ccgcagaagc ggggcgtttg cacgcgcgtg
     3541 tacacgacca cgcccaagaa gccgaactcg gccctgcgaa aggtctgtcg ggttcgcctg
     3601 accaacggct acgaagtcac ctcttacatc ggcggtgaag ggcacaacct gcaggagcac
     3661 tccgtggtcc tgattcgcgg aggtcgtgtt aaggacctgc caggcgttcg ctaccacacg
     3721 gttcgcggaa cgctcgacac ctcgggtgtc tcggaccgca agcagcgccg ttctaaatat
     3781 ggagccaaac ggcccaagta aatcgccgga atattcgttc cagtaaggcc ccggataacc
     3841 tcggggaaac acctgaagcc aaggagcagc taatgcccag acgtcgaatc gtcgccaagc
     3901 gcgaaatcct tcctgatccc aagtacaaca accagacggt cgccaagttc atcaaccacg
     3961 tgatggtgag cggcaagaaa gccgttgcgg agaggatcgt ctacggcgcg ctgtcgcgca
     4021 tcgaagagcg ggacaacccc gacccggtcg agattttcga gaaagctctc gaagcggtgg
     4081 cgccatttgt cgaagtgaaa tcccgccgcg tggggggtgc gacctaccag gtgccggtcg
     4141 aggtccgggc gtcccgacgc gaggccctgg ccatgcgctg gctggtggac agtgcgcgca
     4201 agcgcggaga gaagtcgatg gctgcccgtc tcgccggcga gctaatggat gctgccgacg
     4261 gtcgtggctc cgcggtcagg aagcgggagg atactcaccg catggccgaa gccaaccggg
     4321 cgttctcgca ctatcgttac tagcggttcc cttgtttaag ttcccttcga atttctggag
     4381 agcatgatgg ctcgtacgac ccctatttcc cgataccgga acatcggcat cgttgcgcac
     4441 gtggatgcgg gaaagaccac caccacggag cgggtgctct tctatacggg gctgtcgcac
     4501 aagatcggcg aggttcacga cggcgcggcc accatggact ggatggagca ggagcaggag
     4561 cggggcatca ccatcacctc cgcggccacc acctgctttt ggaggggcat gaaccagcag
     4621 ttcgacgagc accgcatcaa catcatcgat acgcccgggc acgtggactt caccatcgag
     4681 gtggagcgca gcctgcgagt tctcgacggg gccgtggtcg tgttctgcgc cacttcgggc
     4741 gtcgagccgc aatcggagac ggtgtggcgc caggccaacc gctacgaggt gccgcgcatc
     4801 gttttcgtca acaagatgga ccgggccggg gccgactttc tccgggtcgt cgatcagatc
     4861 aagaaccgcc tcggcgccgc gcctgtgccc attcagctgt ccatcggcgc ggaggagcag
     4921 ttcaagggtg tggtcgatct cattcgaatg aaggccatca tctggaacga ggacgaccag
     4981 ggcctcacct atgacctggc ggacattccc gaagaactga tggccgatgc cgaacactgg
     5041 cgcgaacaga tggtggaaaa cgctgccgaa gccaacgagg agctgatgga gaagtacctc
     5101 gaggaagggg agctgtcggc cgaggacatc aaagagggtc ttcgcatacg cacgctcgcc
     5161 aacgagatcg tccccgccct ctgcggctcc gcgttcaaga acaagggcgt ccaggcggtg
     5221 ctggatgcgg tggtggagta ccttcccgca cccaccgagg tgaaagccat cgaaggtctg
     5281 ctccaggacg aagaaacggt ggaatcgcgt caggcggatg acgatgcgcc gtttgcggct
     5341 ctcgctttca agatcgccac ggatccgttc gtgggaacgt tgaccttttt ccgcgtttac
     5401 tcgggcgtgc tgagttctgg cgatcaggtc tacaacaccg tcaagagcaa gcgcgagcgg
     5461 gtaggccgaa tggtgcagat gcattcgaac aaccgcaacg agatcaagga agtgcgcgcg
     5521 ggcgacattg ccgctgccat tggtctgaag gacgtcagca cgggggatac gctctgtgcc
     5581 caggacagcc tgatcaccct ggagcgtatg gagtttccgg agcccgtcat ctccgtggcc
     5641 gtcgagccca aatcggtgcc cgaccaggag aagatgggca tcgcgctcgg caagctggct
     5701 caggaagatc cgtcgtttcg cgtggaaacc gatgaagagt cgggccagac gatcatctcc
     5761 ggcatgggag aacttcatct ggacatcatc gtcgatcgca tgcgtcgcga gttcggggtc
     5821 gaagcaaaca tcggcaagcc ccaggtggct tatcgcgaga ccatccgagc gcccatcgag
     5881 caggaaggca agttcgtccg tcagagtggt ggccgcggtc agtatggtca cgtctggttg
     5941 cggatcgagc cctatgagga cgaggagggc ttggccacct acgagttcgt cgatgagatc
     6001 gtgggcggcg tcgtgccgcg ggaatacatt cccgccgtgg acaagggtat ccgggaacag
     6061 atgcagaatg gcgtgatcgc cggttacccg ctgatcgggg tcagggccat actctacgac
     6121 ggctctttcc acgaggtgga ctcgtcggaa atggcgttca agatcgctgg gtccatggcc
     6181 ctgaaggagg gtgcgcagaa ggcaagtccg gtcctgctcg aacccatcat gaacgttgag
     6241 gtcgtaacgc ccgaggatta catgggcgat gtggtgggcg acctgaaccg gcgccgcggg
     6301 atcattgccg gcatggacga gaacccggcg ggcaagatca tccgcgcgga tgtgccgttg
     6361 tccgagatgt tcgggtactc cacggacctg cgttcggcca cccaggggcg ggcgacctac
     6421 acgatggagt tcgccaagta cgcacaggtt cccgaaagca ttgccgaagg cattatcaat
     6481 cgataaagtt cgccgcttcc cgcggccacc aagaagccgc ttcccgcggc caccaagaat
     6541 agggtaacga cgatgtccaa agcgaaattt gagcgtacta agccacacgt aaacgttggg
     6601 acgattggtc atgtagacca tggtaagacg acgctga
//
LOCUS       DBNHKV010000453         8730 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_630519, whole genome shotgun sequence.
ACCESSION   DBNHKV010000453 DBNHKV010000000
VERSION     DBNHKV010000453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8730)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8730)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8730
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_630519"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="AC16PP_14910"
     CDS             complement(<1..885)
                     /locus_tag="AC16PP_14910"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6482969.1"
                     /translation="MAGVWQELDRIVRPQGQVFYGWWIVLASSGVQWLAAVLWMQSYG
                     AYMVLLQQDFGWSKAMVAGAFALTRIESGILGPLQGWLVDRFGPRAILTIGTVLFGVG
                     FILFGQVESLLTFYLTFALIALGASLGGFATLMVAIVNWFSRHRSKAIAVSQLGFSLG
                     GLSVPLIIWALEAFGWRATAVVSGLLVLVVGLPLVQVVRHRPEAQGELADGHAASGGG
                     PEGRFSLGGDFTARQAMRTRSFWLISFGHALALLTVSAMMVHLVPHLTEGMGYSLSSA
                     GMVVAVLTAFQLLGQLSGG"
     gene            complement(956..1630)
                     /locus_tag="AC16PP_14915"
     CDS             complement(956..1630)
                     /locus_tag="AC16PP_14915"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR6482970.1"
                     /translation="MAGGSSERGDERDALVALIESEVADTADYTGRSKLRPAVLEAMR
                     AVPREEFVPPEEAYAAYANVPLPIGHFQTISQPYVVALMTDLLDPEPGDVMLEVGTGS
                     GYQAAVLSTLVSRVYSLEVVPQLAASAAERLARLGYDNVVTREGDGNLGWPDHAPYDG
                     IVVTAGAPEVPPALIDQLKPGAALVIPVDAGFNQMLRRITRDPDGDVHTEDVLPVAFV
                     PLVKAT"
     gene            1822..2649
                     /locus_tag="AC16PP_14920"
     CDS             1822..2649
                     /locus_tag="AC16PP_14920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6482971.1"
                     /translation="MPKDNADQIEFWNGPTASNWVAAEERMDELLAPITTAILQAAAV
                     SPGEAVLDVGCGCGATSRAMAAVGARVTGVDVSAPMLARARQRADDESLSFIEADAQS
                     HPFDPGFDLLFSRFGVMFFADPVAAFANLRGALNPGGRLCFICWRAPGENPWLSIGMA
                     AAQPFLPPAETPDPRAPGPFAFADPDYLASVLADAGFEDVAITPHNPEMRLGADLTEA
                     MEFVTGVGPLSNVLKELAADLREAALAAVREALAPFAGPSGVLLGGACWIAQGRRGA"
     gene            complement(2675..3865)
                     /locus_tag="AC16PP_14925"
     CDS             complement(2675..3865)
                     /locus_tag="AC16PP_14925"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR6482972.1"
                     /translation="MQTFVTHLECSLTGEHYPADQVHGLSAAGRPLLVRYDLTGLSRA
                     VGKADIAGRQGGFWRYREFLPVRRTENVVSLGEVVTPMIPIAGPAGGSVWVKDEGRLP
                     TGSFKARGLALAVAMARELGLSHLAMPTNGNAGAALAAYAARAGLRATVLCPSDTPEI
                     NVREIAMQGAEVWRVDGLINDCGRIVGEAAGRTGWFDLSTLKEPYRIEGKKTMGIELA
                     EQLGWRLPKAIFYPTGGGTGLIGMWKAFAELEAIGWIGPERPQMFAVQAEGCAPIVRA
                     FERGDEHAELWENAHTAAAGIRVPAAVGDFLILRAVRESGGAALAVSDADLLAAQRRI
                     AAQEGLLLCPEGAATYVAWRQALEAGLVGAEDEVVLFNCATGLKYPMPDESRTFTRLD
                     DLIG"
     gene            complement(3919..4221)
                     /locus_tag="AC16PP_14930"
     CDS             complement(3919..4221)
                     /locus_tag="AC16PP_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family probable chaperone protein"
                     /protein_id="HIR6482973.1"
                     /translation="MLALVFSAVAVAAMGLDAAKAQGLVGEQHDGYLGIVSSATPEVR
                     ALVSDVNAKRRAEYQRIAQTNGIDVKDVELLAGQKAIQRTAPGHYVKLEGQSWRPK"
     gene            complement(4267..4449)
                     /locus_tag="AC16PP_14935"
     CDS             complement(4267..4449)
                     /locus_tag="AC16PP_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HIR6482974.1"
                     /translation="MLLALALALAVLAAAGCTPTVKVATDEPITINLNVNIKHEILVR
                     VDKELNDLFGEDSDLF"
     gene            complement(4594..6750)
                     /locus_tag="AC16PP_14940"
     CDS             complement(4594..6750)
                     /locus_tag="AC16PP_14940"
                     /inference="COORDINATES: protein motif:HMM:NF023167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane phospholipid transport protein
                     YdbH family protein"
                     /protein_id="HIR6482975.1"
                     /translation="MKWLLAIVILLAGLLAGAYASLPWLAETLALREAAAVGIEVREL
                     DVRFPRWGEIAVDRLVASMQGNELRAEQLSVAYNWSGVRERKLAFVGAALLTVYVEDS
                     SGDAEPGALGEQIVVLPGLFLDAWQELPVESVQVDEVRLRTGELVFAGTFGFTPAAAS
                     LAGEVSGVEPLGALRLSMRADAAGSVGLAIQQLERPANSLAVSADARRLAGEAVIAWN
                     LEVPVEDGELAVTLEGRGPIELSENGWRLGSGLQARVDGGAWRGGFAVAEAEGPWPED
                     EAAELVRVSGAWQLEADLPHDVAARGTLSAGLDRSGAKLSIDPGMVVTGAAVSGASWS
                     LEGIRLDTLDGLALEVGIEPMTLTLGSPVRAALAADAARWENFALAPYVDLRIDSLAM
                     DPGSARGAFAATVPALQLSASGQIRADAESSTMVVELSARQRLRAPLLATFLAGWDQP
                     YDVDAGNVAVTGSLTLGDGALSGDVVVTLEGVQSHYGEVVAAGIAGEARVKLREDGWQ
                     ATADTLSVAVADPGFPLRDLRFAAELTPATALLSAVRGRALGGELFIDRLAYDMNDAT
                     AAFLVIATGIELADVLALEGESVTGSGILDGELPVELLEGGTRITNGRFVARAPGGVL
                     RYPDAGKAAEALGQQVGGFALAPLVDFRFEVLDAHVDLQPSGDLLLGVRLEGRSPEFE
                     RPYHYNLNISENIPALLKSLRISEEFQRRLEEVLQR"
     gene            complement(6751..8118)
                     /locus_tag="AC16PP_14945"
     CDS             complement(6751..8118)
                     /locus_tag="AC16PP_14945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR6482976.1"
                     /translation="MSSGVDLSAGPVSRHLVNLTIPMFLGISSMILASMIDTVYVGWI
                     GTRELAAVSFSFPVVLAMATVSMGIGVGAASIIARVVGAGDMERVRRLSTNALYLVAM
                     LVLSLTLLGMLFVEPLFRVLGAGSDILPLSVRYMTVWFLGLPAFALPMVATNIMRAVG
                     SARLPGVIMAAGAGLQVLIAPPLIFGIPGVADGLGITGAAWSFVLSRLAMFGYTVRVM
                     QRMNLLSFAREPWSSRRESWGEVLKIGLPSMVGNLIGPVSMGVVVALLADYGHEVVGG
                     FGVASRIESLATMIIMALAASTGPVIGQNWGARRYERIYRAQSLGFRFSFLWSLLAFA
                     LLAGFGRPIVGFINQDPAVVDATYLYLLLLPATYGLLGVGMVASSTFIALGKPMPTLV
                     LSLLRMIVLYIPMALIMETLFGYAGIFAAAAVCNCLFGGLAYGWVRRMLHKEISRHGR
                     YEGAG"
     gene            complement(8136..>8730)
                     /locus_tag="AC16PP_14950"
     CDS             complement(8136..>8730)
                     /locus_tag="AC16PP_14950"
                     /inference="COORDINATES: protein motif:HMM:NF023284.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3376 domain-containing protein"
                     /protein_id="HIR6482977.1"
                     /translation="DGPLIEKLAVAARNLPLEPDEARDLLKDIFGALGLQSFDREFDA
                     LICTFLDRLTDDRLRDQMLTDYVGFPVFDVLLMSPASLEGGPDPLTPISVERISPEDA
                     PTLRSVFKGLKSRQFMGFLGFFNRAYREHDYLWGRLNAADRLVDLLVQSSRDAISDPD
                     RMRLKLFRAVVDRERQQLYRCDDELDRIDAFLKAAGV"
BASE COUNT         1641 a         3000 c         2803 g         1286 t
ORIGIN      
        1 gccgccggag agctgaccga gaagctgaaa ggcggtgagc acggctacca ccatgcccgc
       61 ggaggacagc gagtagccca tgccctcggt cagatgaggc accaggtgaa ccatcatggc
      121 cgagaccgtg agcagggcca gcgcgtgacc gaaggagatc agccagaacg agcgcgtccg
      181 catggcttgt ctggccgtga agtcgccgcc cagggagaag cgtccctccg gaccgccgcc
      241 ggaggccgcg tgcccgtctg ccagctcccc ctgagcctcc ggtcgatggc gcaccacctg
      301 taccagagga aggccgacga cgagcaccag caggccggac accacggcgg tggcgcgcca
      361 gccgaacgcc tccagcgccc agatgatcag cggcaccgag agcccgccga gggagaagcc
      421 gagctgggat accgcgatgg ccttcgaccg gtggcgactg aaccagttga cgatggccac
      481 catcaacgtg gcgaaacctc ccaggctcgc gccgagcgcg atgagcgcga acgtcagata
      541 gaaggtcagc agggactcca cctggccgaa caggatgaac ccgaccccga agagcaccgt
      601 gccgatggtg aggatcgcgc ggggaccgaa tcggtccacc agccagccct ggagcggtcc
      661 gaggatgccg ctctcgatcc gggtcagggc aaacgccccc gcaaccatgg ctttgctcca
      721 cccgaaatcc tgctgaagga gcaccatgta agccccgtag gactgcatcc aaagcacggc
      781 ggccagccat tggacaccgc tgctggccag cacgatccac cagccataga aaacctgtcc
      841 ctgaggccgc acgatgcggt caagttcctg ccagacgcct gccatgggaa aagatgatac
      901 gccattggtc ggccatccat gacgtcgcag gaagctaagg ttgcgcggac ccctcctagg
      961 tcgctttcac gagcggcacg aatgccaccg gcaggacgtc ctccgtgtgg acgtcgccgt
     1021 cgggatcgcg ggtaatccgc ctgagcatct ggttgaaccc ggcgtccacc ggaatcacga
     1081 gggccgcgcc gggcttgagc tgatcgatca atgccggcgg aacttccgga gcaccggcgg
     1141 tcacgacgat cccgtcgtag ggcgcgtggt ccggccagcc cagattgcca tcgccctccc
     1201 gggtgaccac gttgtcgtat cccagccggg ccagccgctc ggcggcgctg gcggcgagct
     1261 ggggaaccac ctccagggag tagacgcggc tgaccagggt gctcagcacc gccgcctggt
     1321 atccgctccc cgtgccgact tccagcatca cgtcgccggg ctccggatcc agcagatcgg
     1381 tcatgagggc gacgacgtat ggctgcgaga tcgtctggaa gtgtccgatg ggcagcggca
     1441 cgttggcgta ggcggcgtag gcttcctccg gaggcacgaa ctcttcccgc ggcacggcgc
     1501 gcatggcttc gagcacggcg ggtcgcagct tcgagcggcc cgtgtaatcg gcggtatccg
     1561 ccacttcact ctcgatcagc gcaacgagcg cgtcgcgctc gtccccacgt tcggaagatc
     1621 cccctgccat cgcttctgat caccccgccg gcatcgccct tgcctcgagt ttacccgagc
     1681 ccgcgcaccg ggccattgag ggactccgaa aaaaggcggg catccctggg cagggtgacc
     1741 gggcggcggc gtgcttctag aatctctctt cgccgtgcgg tttagccccg cacccaccgg
     1801 aaacacactg acgaaataga catgccaaaa gacaacgccg atcagatcga attctggaac
     1861 gggcccacag ccagcaactg ggtggccgca gaggaacgca tggacgagct gctggccccc
     1921 attaccaccg ccatactcca ggccgcggcc gtgagccccg gcgaggccgt cctcgatgtg
     1981 gggtgcggtt gcggcgccac gtcgagggcc atggcagcgg tcggagctcg ggtgaccggt
     2041 gtggacgtat ccgcgcccat gctcgcccgg gcacgacaac gcgccgacga cgagtccttg
     2101 agcttcatcg aagcggatgc ccagagccac cccttcgatc ccggtttcga tctcctgttc
     2161 tcccggttcg gggtgatgtt cttcgccgat ccggtagcgg ctttcgccaa cctgcggggc
     2221 gcgctgaacc ccggggggcg cctgtgtttc atctgctggc gggcccctgg ggagaatccc
     2281 tggctgagca tcggcatggc cgccgcgcag cccttcctgc cgccggcgga gacgcccgac
     2341 ccccgagcgc cggggccgtt tgccttcgcc gatccggatt atctggcttc ggtgctggcc
     2401 gatgccgggt tcgaagacgt ggccatcaca ccccacaatc ccgagatgcg cctgggtgca
     2461 gacctgacag aggccatgga gttcgtgaca ggggtcggac cgttgagcaa cgtcctcaag
     2521 gagctggctg cggatctgcg ggaggccgcg ctggccgccg tgcgggaggc tttggcgccc
     2581 ttcgccggcc cctcaggcgt cctgctgggg ggcgcgtgct ggatcgccca agggcgtcgg
     2641 ggcgcctgaa cggcgggcgg gtcgcccgcc gggatcagcc gatgaggtcg tcgagcctgg
     2701 tgaaggtccg gctctcgtcg ggcatgggat acttcagccc cgtggcgcag ttgaagagaa
     2761 cgacctcgtc ctcggcgccg accaggcccg cctccagggc ctggcgccag gcgacgtagg
     2821 ttgcagcgcc ctcggggcag agcagcagac cctcctgcgc cgcaatgcgc cgctgggcag
     2881 cgaggaggtc tgcatcgctc accgccaggg cagcgccccc ggactcgcgc acggcgcgca
     2941 gaatgagaaa gtcgcccacg gcggcgggca cccggatacc ggctgccgcc gtgtgcgcgt
     3001 tctcccacag ctcggcatgc tcgtcgccgc gctcgaacgc gcgaacgatg ggggcacagc
     3061 cttcggcctg cacggcaaac atctgcggtc gctccggccc gatccagcca atagcctcga
     3121 gctcggcgaa cgctttccac atgccgatca gaccggttcc accgccggtc gggtagaaga
     3181 tggccttggg caggcgccag ccgagctgct cggcgagctc tatgcccatc gtcttcttgc
     3241 cctcgatccg gtagggctct ttcagggtgg aaagatcgaa ccaaccggta cgtccggccg
     3301 cctcgcccac gatgcgcccg cagtcgttga tgagcccgtc cacgcgccat acctccgcgc
     3361 cctgcatcgc gatctcgcgc acgttgatct ccggggtatc cgagggacac agcacggtgg
     3421 cccggaggcc ggccctcgcc gcataggccg caagtgccgc cccggcgtta ccgttggtgg
     3481 gcatggccag gtgggaaaga cccagctccc tggccatggc cacggccaga gcgaggcctc
     3541 ttgccttgaa cgaaccggtg ggcagtcggc cctcatcctt gacccagacg ctgccgccgg
     3601 cagggccggc aatcgggatc atgggggtga cgacttctcc cagggagacc acgttctccg
     3661 tgcgacgcac gggcagaaac tcccggtaac gccagaagcc gccctggcgg ccggcgatgt
     3721 ccgccttgcc caccgctctc gaaaggcccg tcaggtcgta tctgacgagc agcggtcgcc
     3781 ccgcagcaga gaggccgtgg acctgatcgg ccgggtagtg ctccccggta agcgagcact
     3841 cgagatgggt aacgaaggtc tgcaaagcgg tttaggactg gggttgggcg agcccgctct
     3901 cgcagccagg ctcgccggct atttcggccg ccagctctga ccttcgagct tgacgtagtg
     3961 accgggggcg gtgcgctgaa tggccttctg gccggcaagc agctccacgt ccttgacgtc
     4021 gatgccgttg gtttgagcga tgcgctgata ctccgcgcgc cgcttggcat tgacatcgga
     4081 gacgagggcc ctgacttcag gcgtggcgga cgagacgata cccaggtaac cgtcgtgctg
     4141 ctctccgacc agaccctgtg cttttgccgc atccaggccc atggccgcaa cggccactgc
     4201 cgagaagacc agcgcgagca tgaaaacgat ttgccgaaag attggtttca tattcatcgc
     4261 gtcgccttaa aagagatcac tgtcttcgcc gaacaggtca ttcagctctt tgtccactct
     4321 cacgagaatc tcgtgcttga tgttgacgtt aaggttgatg gtgatgggct catccgttgc
     4381 gaccttcacc gtcggggtgc atcccgcagc ggcgagaacg gcgagagcaa gggcgagcgc
     4441 gagcagcatc cctgaggcgg ccgtacgcgg cctgaccggt gggatccggc ggcaggaaac
     4501 cggcaattcg aacgagttac tcatcatcaa agagtacctg gcgcactgtg aaccggagct
     4561 gaacattacc cagcgccgac accccccgca agctcacctt tgcagaacct cttccaggcg
     4621 tcgctggaac tcttcggaaa tgcgcaggga tttgagcagg gccggaatgt tctccgagat
     4681 gttcaggttg tagtgatagg gccgctcgaa ttccggactg cgcccttcca accgaacccc
     4741 gagcaggagg tcgccgctcg gctggagatc gacatgcgca tcgagcacct cgaatcggaa
     4801 atccaccagc ggcgcgagag cgaacccgcc gacctgctgg ccgagcgctt cggccgcctt
     4861 ccccgcatcc ggatagcgca gaacgccccc gggcgcccga gcgacgaaac gtccgttcgt
     4921 gatcctggtg ccgccctcga ggagctcgac aggcagctcg ccatcgagaa tgcccgatcc
     4981 ggtcaccgac tctccttcca gagccagcac gtcggctagc tcgatcccgg ttgcgatcac
     5041 cagaaaagcg gcggtggcgt cgttcatgtc gtaggccagt cggtcgatga agagctcccc
     5101 gccaagcgct ctgccccgaa ccgcgctgag cagcgccgta gccggcgtca gctccgcagc
     5161 aaaccgcaga tcacgcagag gaaagccggg gtctgcaacc gccacgctca gcgtatcggc
     5221 cgtagcctgc cagccgtctt cgcgcagctt cacccgggcc tcaccggcaa tgcctgccgc
     5281 caccacttcg ccgtagtggg attgaacgcc ttcgagggtc acgaccacgt cgcccgacag
     5341 cgccccgtca ccgagggtca ggctgcccgt cactgcgacg tttcccgcgt cgacgtcgta
     5401 aggctgatcc cagccagcaa ggaacgtggc cagcagcggc gctctcagcc gctggcgagc
     5461 cgatagctcg acgaccatgg tcgacgactc ggcgtccgca cgaatctgcc cagaggcgga
     5521 gagctggagg gcggggaccg ttgccgcaaa cgcgccccgc gcgctgccgg ggtccatggc
     5581 gagggaatcg atgcgaaggt cgacgtaggg agcaagtgcg aagttctccc agcgagcggc
     5641 atccgcagcc agcgcggcgc gaaccgggct ccccagggtg agcgtcattg gttcgatccc
     5701 gacttcgagc gcaaggccgt cgagggtatc cagacggata ccctcgagac tccaagacgc
     5761 acccgaaacg gcggcaccgg tcaccaccat gcccgggtcg atggacagct tcgcgccgga
     5821 tctgtccaac ccggccgaca gggttccacg cgccgcaacg tcatggggca ggtcagcttc
     5881 gagctgccag gcgccggaga cacggacgag ctcggcagcc tcgtcttcag gccaggggcc
     5941 ctccgcttcg gccaccgcga agcctccgcg ccaagccccg ccgtccacgc gcgcctgcag
     6001 cccggatccc aaccgccagc cattctcgct aagctcgatg gggccccggc cttccagggt
     6061 gacggcgagt tcgccatcct caacgggcac ctccagattc caggcaataa ccgcctcgcc
     6121 cgccagacgc cgagcatcgg ccgagacagc cagggagttg gccggtcgct ctagctgctg
     6181 gatggccagg ccaaccgagc ccgcggcatc tgcgcgcatg gaaagccgga gcgcgccaag
     6241 gggctcgacg ccgctcacct cgcctgccag gctggctgcc gctggcgtga atccaaacgt
     6301 accggcaaag acgagctctc ccgtgcgcaa acggacctca tccacctgaa cgctctccac
     6361 cggcagttcc tgccaggcgt ccaggaacag gcccggcagc acaacgatct gctctccaag
     6421 cgccccgggt tccgcatcgc cgctggagtc ctcgacgtag accgtcagca gcgcagcgcc
     6481 cacgaacgcc agctttcgct cccggacgcc cgaccagtta taagcgacgg acagttgctc
     6541 ggcccgcagc tcattgccct gcatgctcgc gacgagccga tccacggcaa tttcacccca
     6601 gcgcggaaat cgcacgtcca gctcccgcac ctcgatgccg acagcggccg cctcgcgcag
     6661 ggccagcgtc tcggccagcc agggcagcga cgcgtaagcg cctgccagca ggccggccag
     6721 aagaatcacg attgccagga gccacttcac ctacccggcc ccttcgtagc gcccatgacg
     6781 ggaaatctcc ttgtgcagca tccggcgcac ccaaccatag gccaggccgc cgaacaagca
     6841 gttgcagacg gccgccgcgg caaagattcc cgcatagccg aagagcgttt ccatgatcaa
     6901 agccattggt atgtagagaa cgatcattcg caacagcgaa agcaccaggg tgggcatggg
     6961 cttgcccaac gcgatgaagg tcgagctcgc caccatgccc acaccgagaa ggccgtaggt
     7021 agctggaagc aacaacaggt agaggtaggt tgcatcgacg acggccgggt cctggttgat
     7081 gaagcccacg atgggccggc cgaaaccggc gaggagggcg aaagccagca gtgaccacag
     7141 gaagctgaag cggaacccca gggattgcgc ccggtagatg cgctcgtagc ggcgagcacc
     7201 ccagttctgg ccaatcaccg gtcccgtgct ggcagccagg gccatgatga tcatggtagc
     7261 cagggactcg atgcgcgagg cgacgccaaa cccgcccacc acctcgtgcc cgtagtcggc
     7321 cagcagggcc accaccacgc ccatggagac cgggccgatg aggttgccga ccatggacgg
     7381 caggccgatc ttaagaactt ccccccagga ctcgcgacgc gaagaccagg gctcacgggc
     7441 gaacgacaac aggttcatgc gctgcatcac cctgaccgtg tagccgaaca tggcgagccg
     7501 agacagcacg aatgaccaag ccgccccggt gattcccagc ccgtccgcca cgccggggat
     7561 accgaagatc agcggcggcg cgatgagcac ctgaaggccg gcacccgcgg ccatgatgac
     7621 gcccggcagg cgcgcgctgc ccaccgcgcg catgatgttg gtggcaacca tgggcagcgc
     7681 aaaagccggc aggccgagaa accacaccgt catgtagcgc accgacagag gcaggatgtc
     7741 ggaccctgcg cccagcaccc tgaagagcgg ctcgacgaaa agcatcccga gcagggtgag
     7801 gctcaatacc agcatggcga ccaggtagag cgcgttcgtc gacaggcgcc gcacccgttc
     7861 catgtcgccc gcccccacca cccgggcgat gatggacgcc gccccgaccc cgattcccat
     7921 ggagaccgtc gccatggcca ggaccacggg aaaggagaag ctcaccgccg ccagctcccg
     7981 ggtgccgatc cagccgacgt agaccgtatc gatcatcgac gcgaggatca tcgacgagat
     8041 cccgaggaac atggggatcg tcaggttgac caggtggcgg gatacggggc ccgcggacag
     8101 gtcaacgccc gagctcacga atccggtctc atccgctaga cgcccgcggc tttcagaaac
     8161 gcatcaatcc tgtcgagctc gtcatcgcac cggtacagct gctgacgctc gcggtcgacg
     8221 acggcgcgaa agagtttgag gcgcatccga tcggggtcgc tgatggcatc acgagacgac
     8281 tgaacgagca gatcgaccag ccggtccgcg gcgttgagcc tgccccagag gtaatcgtgc
     8341 tcgcgatagg cccggttgaa gaaaccgaga aaacccatga actggcgcga cttcagtcct
     8401 ttgaagaccg acctgagcgt cggcgcgtcc tccgggctga tcctctccac gctgatgggc
     8461 gtgagcgggt cggggccgcc ctccagggac gccggcgaca tgagcagcac gtcgaaaacc
     8521 ggaaagccga cgtaatccgt gagcatctgg tcgcgcagcc ggtcgtcggt aagccggtcg
     8581 aggaaggtgc agatcagggc atcgaactcg cggtcgaacg actgcagccc cagcgcgccg
     8641 aatatgtcct tgagtagatc cctggcctcg tccggctcga gcggcaggtt ccgggcggcc
     8701 accgcgagct tttcgatcag cgggccgtcg
//
LOCUS       DBNHKV010000454         3587 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1056814, whole genome shotgun sequence.
ACCESSION   DBNHKV010000454 DBNHKV010000000
VERSION     DBNHKV010000454.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1056814"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="AC16PP_14955"
     CDS             <1..941
                     /locus_tag="AC16PP_14955"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6482978.1"
                     /translation="AAKAVMGQTPQPLDGMRVVVCGSASPLGNDPNRAQACIAVVTPE
                     HFFLFDVGARSPVRMAQARLPMGRIDGVFLTHFHSDHIAALPDVNLASWVQSRPAALT
                     VYGPPGVGAVVDGFNTAYRLDRGYRTAHHGAELLPPERGPMLAKTFVPGDVVWRDDLM
                     TVTSFEVEHPPIEPAVGYRVDYRGRSVVISGDTNAADTLFTVAKDADLLLHDALSRTL
                     LDPMIATATELGVPVLPTIMTDVIDYHADVTSLPARAAEAGIRQLALYHMVPVPPNAL
                     AAKMFLRGIPDDVIVTRDLHTFDLPANSTDIVISEP"
     gene            945..2231
                     /locus_tag="AC16PP_14960"
     CDS             945..2231
                     /locus_tag="AC16PP_14960"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR6482979.1"
                     /translation="MKLKTRLRSAAMRQLSSAAYLEKQRAKARKLRQRKGEPPVVHYF
                     HQVDDPYSHLAVQKLDELDDRYDLAFQPHLASKPGEDYRGSAAHFDHWARKDAASVAA
                     DYGTRFAPAINAPAGDAVDAANRVLSAHLDRDDFARAAVSIGEALWSDQRIAEPADAH
                     DGLRAVAEGNDLRRALGHYQGAMFYFEGEWFWGVDRIRLLERRLIDEGFGSSETLCVP
                     EPTPTETSGLDTSSVLLEYFPSLRSPYTAIGHARVLDLIARSGVCVRVRPVMPMMMRG
                     IPAPRAKQRYIITDAGREGRAHGAPFGPIVDPFGDPVKRAFALLPGAAAAGREMEYVT
                     AYLNAAWFEGVDITTQKGLRAVATNAGMDWAALEQASRQENWEAILEENLKTMLKEDL
                     WGVPSFRVSGGNSDEAFACWGQDRIWRVENEIASRT"
     gene            2292..2930
                     /locus_tag="AC16PP_14965"
     CDS             2292..2930
                     /locus_tag="AC16PP_14965"
                     /inference="COORDINATES: protein motif:HMM:NF037008.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HIR6482980.1"
                     /translation="MRAAEKLFAERGIENVSIRDIVGEAGQKNESALQYHFKSLTGLI
                     SAIHAERSAETQARRGQLLEAMLAQTPDPPLRQLCILMVRPAFDLARGDVGFRRYIKA
                     FGQELALTETSALARAVAHGGGGTSGQQLAGLLKRALPHLDEEAYQRRMEAAVRLCSA
                     SVFHQARQRHAFRGQQADLFFHSLIDALVGLLSAPVSQETRAVAESLGRPEA"
     gene            2975..>3587
                     /locus_tag="AC16PP_14970"
     CDS             2975..>3587
                     /locus_tag="AC16PP_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482981.1"
                     /translation="MTRIVSRAVLTLVVVTLGAFCFAFFSPRPALTIDPATLAGDGSA
                     IDYCDLPLLDGSGKAAADIPKGNTPGCGYDHFPLPILASCTEQLTEGAADIRGLWVGV
                     EGGFVGHVERVEQCGSRTVVTSSGIIHDSGPNSTAGLNTNDTEGAVLFIIGEKEYCPR
                     TSASMIWNEGVLEFHAFGWGPVVVRRYLEGERLVWEYLDGSVTR"
BASE COUNT          644 a         1186 c         1174 g          583 t
ORIGIN      
        1 gcgccgccaa ggccgttatg ggccagaccc cacagcctct ggacggcatg cgcgtggtcg
       61 tgtgcggcag cgcgtcgccg ctcggcaacg atccgaatcg ggctcaggca tgcattgccg
      121 tcgttacccc ggagcacttt ttcctgttcg acgtgggcgc gcgctcgccg gtcaggatgg
      181 ctcaggcgcg attgcccatg ggccggatcg acggggtgtt tctgacgcac ttccactccg
      241 atcacattgc cgcgctgccc gacgtgaacc tggcctcctg ggtgcagagc agaccggccg
      301 cgctgacggt gtacggtccg ccgggagtcg gggcggtggt ggacggattc aataccgctt
      361 accggctaga ccgcggctac cgcacggctc accacggcgc cgagctgttg ccgcccgaac
      421 gcggcccgat gctggcgaaa acgttcgtcc ccggcgacgt cgtctggcgc gacgacctga
      481 tgacggtgac ctcgttcgaa gtggagcatc cgcccatcga gcccgcggtg ggctaccgcg
      541 tcgactatcg gggacgctcg gtggtgatct ccggagatac caatgcggcg gacaccctgt
      601 tcaccgttgc caaagacgca gacctgctgc tgcacgacgc gctgtcgcgt acgctgctgg
      661 acccgatgat tgccaccgcc accgagttgg gcgttccggt ccttccgacc atcatgaccg
      721 acgtcatcga ctatcacgcc gacgtcacca gcctgcctgc gcgcgcggcg gaagccggca
      781 tcaggcagct ggccctgtac cacatggtgc ccgtcccacc caacgcgctg gcggcaaaga
      841 tgttcctcag gggcattccg gacgacgtca tcgtcacgcg ggatctgcat accttcgatt
      901 tgcccgcgaa ctccacggac atcgtcatta gcgagccttg agccatgaaa ctcaaaaccc
      961 gactgcgctc ggctgccatg cgccagctct cctcggccgc ctatctggag aaacagaggg
     1021 caaaggcgcg aaagctgcgg cagaggaagg gcgagccgcc ggtcgtgcac tacttccacc
     1081 aggtggacga tccctacagt cacctggcgg tgcagaagct cgacgagcta gacgaccgct
     1141 acgatctcgc ttttcaaccc cacctggctt ccaagccagg cgaagactac cggggcagcg
     1201 ccgctcactt cgatcactgg gcacgcaagg acgccgcaag cgtggccgcg gattacggca
     1261 cccggttcgc gcccgccatc aacgcgcccg ccggcgatgc cgtggatgcg gcaaaccggg
     1321 tgctgtcagc tcacctggat cgcgacgatt tcgcccgcgc tgccgtcagc attggcgagg
     1381 cgctgtggag cgatcagaga atcgccgagc cggccgacgc tcacgacggc ctccgggccg
     1441 ttgccgaagg aaacgacctg cgccgcgcgc tcgggcatta ccagggagcc atgttctact
     1501 tcgaagggga atggttctgg ggtgtcgacc gaattcgtct gctggagcga aggctgatcg
     1561 acgagggctt cggcagcagc gagacccttt gcgtgcccga gccgacccca acggaaacca
     1621 gcggcctcga caccagttct gtgctgctgg agtacttccc gtctctgcgc agcccctaca
     1681 cggccatcgg tcacgcccgc gtgctcgacc tcatcgcacg cagcggggtt tgcgttcgag
     1741 tgcggccggt gatgccgatg atgatgcggg gcattcccgc gccgcgcgcg aagcagcgct
     1801 acatcatcac ggacgccggc cgcgaaggga gagcacatgg cgcgccgttc ggacccatcg
     1861 tcgatccgtt cggggatccg gtgaagcgtg cctttgctct gctccccggt gcggccgcag
     1921 ccggccggga gatggagtac gtcaccgcat acctcaacgc cgcctggttc gagggcgtgg
     1981 acatcacaac gcagaaaggc ctgagagcgg tggcgacaaa cgcgggaatg gattgggccg
     2041 cgctcgagca ggccagccgg caggagaact gggaagccat actggaggaa aacctcaaaa
     2101 cgatgctcaa agaagacctt tggggcgtgc cgagttttcg agtctcgggc ggaaacagtg
     2161 acgaggcctt cgcgtgctgg ggacaggacc gcatctggcg ggtcgagaac gaaatcgcga
     2221 gccgaacgta gatgccggcg cggagccaag cgggtagaca ctcgagaagc agagccaccc
     2281 aggacgcgct gatgcgcgcc gcggaaaaac tgttcgcgga acgaggcatc gagaacgtgt
     2341 cgattcgcga catcgtgggc gaagcgggtc agaagaacga gtccgcgctg cagtaccatt
     2401 tcaagagcct caccggcctc atctctgcca ttcacgccga gcgatcggcg gaaacccagg
     2461 cccggcgcgg acagctgctc gaagcgatgc tggctcagac acccgacccc ccgctacgcc
     2521 agctctgcat tctgatggtc aggcctgcat tcgacttggc ccgcggtgac gtgggtttcc
     2581 gtcgctacat caaagcgttc gggcaggagc tggctctcac ggagacctcg gctctggctc
     2641 gagcggtcgc ccacggcgga ggtggaacca gcggacagca gctggccggg ctgctgaaac
     2701 gggcgctgcc tcacctggat gaggaggcct atcaacgccg gatggaggca gcggtgcgcc
     2761 tgtgttccgc ttccgtgttc catcaggcca gacagcggca cgcctttcgc ggccaacagg
     2821 cagatctgtt cttccacagc ctcatcgacg cgttggtggg gctactgagc gcgcccgttt
     2881 cccaggagac ccgggcagtc gccgaatccc tcgggcggcc cgaggcgtga ggtttgggtc
     2941 gcgcaatgca gcggcccggc gctgacaggc tgtcgtgacg aggatcgtct cccgcgctgt
     3001 ccttaccctc gtcgtcgtta ccctgggcgc gttctgcttc gcgttcttct ccccccgccc
     3061 agccctgacc atcgacccgg caaccctggc cggggacggc agcgccatcg actactgcga
     3121 tctgccgctc ctcgacggca gcgggaaggc agccgccgac attcccaaag gcaacacgcc
     3181 cggctgcggc tacgatcact ttcccctgcc gatactggca agctgtaccg agcaactgac
     3241 ggagggtgca gccgacattc gaggcctgtg ggtcggggtg gaaggcgggt tcgtcgggca
     3301 cgtggaacgc gtggagcagt gcggctcccg cacggtcgtg accagcagcg gcatcattca
     3361 cgactccgga ccgaacagca cggcaggcct gaacaccaac gacaccgagg gcgccgtgct
     3421 cttcatcatc ggtgaaaagg aatactgccc gcgcacctcg gccagcatga tctggaacga
     3481 gggggtactc gagttccacg ccttcggctg gggacccgtc gtggtgcgtc gctacctgga
     3541 gggcgagcgg ctggtctggg aatacctgga cggcagcgtg acccgaa
//
LOCUS       DBNHKV010000455         2746 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1766503, whole genome shotgun sequence.
ACCESSION   DBNHKV010000455 DBNHKV010000000
VERSION     DBNHKV010000455.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1766503"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1608
                     /locus_tag="AC16PP_14975"
     CDS             <1..1608
                     /locus_tag="AC16PP_14975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR6482982.1"
                     /translation="ADPRTYASCLLSVNGRDEAALMLQGRLQGYQTRFGDLLKPLELF
                     IDLADDEGIEAFLADPEVAPAEFVVRHARKRRHERLGLAEEQLINGLSQEGIHAWGNL
                     YDQLSGTLTCEVLVGNETREMGIAEAAGLLQKPDVATRRNAWRAINDSWSAHAETCAA
                     SLNAIAGWRLELGRRRGREAPVHFLDAPVHMNRISRETLDTLMDVAAGSRPLARRAAR
                     LMARAYGLETIGPWDQRAPAPDLGTAPAAGIPYDQALHTIADAYADVHPSMGEFVRMM
                     HANRWIEGTRGPNKRPGAYCTGFAKSRTPRIYMTYSGGSSDVITLAHELGHALHSWVM
                     RDLPNSQRGYGMALAETASTFGETLVRDALLGSAGSPQAKLDMAWEEMAALVSFVLNI
                     PTRFEFERRFYEARRERPVLPRELNAMMADAWTECFGDVLEEPDPMFWASKLHFYISG
                     ISFYNFPYLFGYLFSLGVYARRGDGADFYPRYEGLLRDTGRMTAEALAAKHLSEDLSR
                     PGFWEATVASLETRVDAFEDLLDELAL"
     gene            complement(1605..>2746)
                     /locus_tag="AC16PP_14980"
     CDS             complement(1605..>2746)
                     /locus_tag="AC16PP_14980"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HIR6482983.1"
                     /translation="TYIGAMPGRILQGVRRAGVTNPILMLDEVDKLGRDFRGDPAAAL
                     MEILDPAQNREFRDNYLNLPYDLSRVLFITTANTLEGIPRPLLDRMEVLDLTGYSDLE
                     KQAIARQYLIPRQRSEAGLSEAQLDISDDALTTMIRRYTREAGVRGLERVIGRLARKR
                     ARQVVDGQSLDTPVAADDLAELLGPERFKSDRGRTGLEPGVAAGLAWTEAGGDVLYVE
                     ATLTQKDEKVTLTGHLGQVMQESARAARSHLWSAADRLGLDRPAIEESGVHIHVPAGA
                     VPKDGPSAGVTMACALYSAYKGRALRDDVAMTGELTLSGLVLPVGGVKEKILAAHRAG
                     LRRVILPKDNEADLAKLPQTVLDEMQISLAAHLDDVLAVAFAEES"
BASE COUNT          416 a          932 c          923 g          475 t
ORIGIN      
        1 gcggacccac gcacttacgc ctcctgcctg ctctcggtga acggccggga cgaagccgcc
       61 ctcatgctgc aggggcgttt gcagggttac cagacgcgct tcggggatct gctcaagccg
      121 ctggagctgt tcatcgatct ggcggacgac gagggcatcg aagcgtttct ggccgacccg
      181 gaggtggcgc cggccgagtt cgtggtgcgc catgcgcgca agcggcgcca tgagcgtctg
      241 gggctggccg aggaacagct catcaacggc ctctcccagg agggcattca cgcctggggc
      301 aatctctacg atcagctctc cggcacgctc acctgcgaag tgctggtggg caacgagacc
      361 cgggagatgg gcatcgccga agcggccggc ctgctgcaga agcccgacgt ggcgacccgg
      421 cggaacgcct ggcgcgcgat caacgactcg tggtcggccc atgccgaaac ctgtgctgcc
      481 agtctcaacg ccatcgccgg ttggcggctg gagctcgggc gccggcgcgg ccgcgaagct
      541 cccgtacact tcctggatgc gccggtgcac atgaaccgga tcagccgcga aaccctggat
      601 accctgatgg acgtggctgc cggctcgcgg cccctcgccc ggcgggcggc gcggctgatg
      661 gcccgtgctt acggcctgga aaccatcggt ccctgggacc agcgcgcgcc ggcacccgac
      721 cttggtacgg cccccgccgc ggggatcccg tatgaccagg cgctgcacac catcgccgat
      781 gcctatgcgg acgtgcatcc ctccatgggc gagttcgtcc gcatgatgca tgccaaccga
      841 tggatcgagg ggacccgggg ccccaacaag cgtccgggcg cctactgcac gggcttcgcc
      901 aagtcccgca cgccgcgtat ctacatgacc tactccggtg gctccagcga cgtcatcacc
      961 ctggctcacg agcttggcca cgccctgcac agctgggtca tgcgggacct gccgaacagc
     1021 cagcgcggct acgggatggc gctggccgag acggcaagca cttttggtga gactctggtt
     1081 cgggacgcgc tcctcggcag tgccggctct ccccaggcaa agctcgacat ggcctgggaa
     1141 gagatggcgg cgctggtgag cttcgtcctc aacatcccca cccgtttcga gttcgaacgg
     1201 cgtttctatg aagcccgccg ggagcggccc gtgctgcccc gggagctcaa cgccatgatg
     1261 gcggatgcgt ggacagagtg tttcggcgac gtgctcgagg agcccgaccc gatgttctgg
     1321 gccagcaagc tgcacttcta catatccggc atcagcttct acaactttcc ctacctgttc
     1381 ggatacctgt tcagcctggg cgtctacgcg cggcgcggcg acggtgccga cttctacccc
     1441 cgttacgaag ggctgcttcg ggacaccggg cggatgacgg ccgaggcgct cgcggccaag
     1501 catctttccg aggacctgtc gcggcccggt ttctgggagg ccacggtcgc atctctcgag
     1561 acccgggtcg atgctttcga ggatctgctc gacgagctgg cgctctagct ttcctcggcg
     1621 aaggccactg ccaggacgtc gtcgaggtgg gcggccagcg agatctgcat ctcgtccaga
     1681 accgtctgcg ggagcttggc aaggtcggct tcgttgtctt tgggcaggat gacccgacgc
     1741 agcccggcgc ggtgcgcggc gaggatcttc tccttcaccc cgccgacggg cagcaccagg
     1801 ccggacagcg tcagctctcc ggtcatggcg acgtcatcgc gaagggccct gcctttgtat
     1861 gccgagtaga gcgcgcacgc catggtgaca ccggcggaag gcccatcttt cggcaccgca
     1921 ccggctggaa cgtgtatgtg cacaccgctt tcctcgatgg cgggcctgtc caggccgagc
     1981 ctgtccgccg ctgaccacag gtggctgcgg gccgccctgg cagattcctg catcacctgc
     2041 ccgagatgcc cggtgagcgt gaccttctcg tccttctggg tgagcgtcgc ttcgacgtag
     2101 agcacgtccc cgcccgcctc ggtccaggcc agccccgccg cgacgccggg ctccagcccg
     2161 gtgcgtccgc ggtcggattt gaatcgctcc ggtcccagca gttcggccag atcgtcggct
     2221 gcgaccggtg tgtccaggct ctggccgtcc accacctgac gcgcgcgctt tctcgccaga
     2281 cggccgatga cgcgctccag cccccgcacg cccgcttcac gggtgtagcg gcggatcatg
     2341 gtggtgagcg cgtcgtctga gatatccagc tgagcctcgg aaaggcccgc ctcgctgcgc
     2401 tgtcggggga tcaggtactg gcgcgcaatg gcctgcttct cgaggtcgct gtagccggtg
     2461 agatccagca cctccatccg gtccagcagc ggccgcggga tcccctccag ggtgtttgcc
     2521 gtggtgatga agaggacgcg tgacaggtcg taaggcagat tcaggtagtt gtccctgaac
     2581 tcgcggttct gggcgggatc gagtatctcc atcagggccg cggcggggtc gccgcgaaag
     2641 tcgcgaccga gcttgtccac ctcgtcgagc atcaggatcg ggttggtgac gccggcgcgc
     2701 cggacgccct ggaggatgcg gccgggcatg gcgccgatgt aggtgc
//
LOCUS       DBNHKV010000456         2337 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_630759, whole genome shotgun sequence.
ACCESSION   DBNHKV010000456 DBNHKV010000000
VERSION     DBNHKV010000456.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_630759"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="AC16PP_14985"
     CDS             <1..291
                     /locus_tag="AC16PP_14985"
                     /inference="COORDINATES: protein motif:HMM:NF013424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HIR6482984.1"
                     /translation="AQAARALARQVREGECSLEEIDEERFAAHLSLGGLPDPDICIRT
                     GGDHRISNFLLWDFAYTELYFTDTYWPDFDAACLHEALNWFDDRQRRFGRRT"
     gene            372..1163
                     /locus_tag="AC16PP_14990"
     CDS             372..1163
                     /locus_tag="AC16PP_14990"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR6482985.1"
                     /translation="MLRTRILTALIIAPLAVAALYLLPAFWFAVAYGLVAALGAYEWG
                     GLIGLRRFWSRLAYAALFVVAAGMTWWLDRGFGLAIVVGSIVWIVAAGLVLTYPATAA
                     VVREPWSNGLLGLFVLWGAWSAVVEVRGEPGSGGANWVMWLLVLVWSADVGAYFAGRR
                     FGRRKLAPAVSPGKTWEGAFGGMLVSLALCGGALLVLGLPAWWLLAVAGLVVVSIFGD
                     LFESVIKRVRGVKDSSNLLPGHGGILDRIDSLLAVLPVFALILAR"
     gene            1284..2294
                     /gene="rseP"
                     /locus_tag="AC16PP_14995"
     CDS             1284..2294
                     /gene="rseP"
                     /locus_tag="AC16PP_14995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR6482986.1"
                     /translation="MDFLQYPLALLVTLGVLVTFHELGHFVIARWSGVTVVRFSVGFG
                     KPFWSWRDRRGTEFALAYIPLGGYVRMLDEREPGVIEYDRRSTDVSYNELSVWWRMAI
                     ALGGPAANFLLAIIVYWVLFVIGTITYAPLLGSVGADSAIGRAGLREHEELIAVDGVE
                     TSSWQAVNMALAGRLGESGEIELTTRRPGSDSVRSGAVPISSWHTGVDEPDLLGSLGI
                     VPALPPIVAQVVPDSPAERAGFRRWDRIVSVDGEAVADWGQWVDLIRNAPDRRLSVTV
                     DRSGQVLVLDVTPDVQEAAGGEVTGFLGVGAYTKEIRYGVLGGDRLVSGRASSSRCPL
                     PR"
BASE COUNT          315 a          724 c          815 g          483 t
ORIGIN      
        1 gcccaggccg cccgcgccct tgcgcggcaa gttcgcgagg gcgaatgcag cctggaggaa
       61 atcgacgagg aacggtttgc cgcccatctc tccctgggcg ggctacccga tcccgacatc
      121 tgtatccgga cgggcggaga tcatcggatc agcaatttcc tgctctggga tttcgcctac
      181 accgaactct acttcaccga cacgtactgg ccggatttcg atgcggcctg cctgcatgaa
      241 gcgctgaact ggttcgatga ccgccagcgt cgcttcggcc gtcgaacatg acgagcccgt
      301 tgcccgggca cgagcggcga gctctacccc ggcagccgtc ctcaccgatg cgaccgacag
      361 tggtctgagc cgtgctgcgg acccggatcc tcactgccct gatcatagcg ccgctcgccg
      421 tcgctgcgct gtatctgctc cccgccttct ggttcgccgt tgcctacggc ctggtggctg
      481 ccctgggcgc ctacgagtgg ggggggctca tcgggctgcg ccggttctgg tcgcgtctgg
      541 cttacgccgc gctgttcgtg gtggccgccg ggatgacctg gtggctcgat agagggttcg
      601 ggctggccat tgtggtcgga tccatcgtct ggatcgtggc ggcgggcctg gtattgacat
      661 accccgcgac cgctgccgtt gtccgggaac cctggagcaa cggcctgttg ggcctgttcg
      721 ttctctgggg cgcatggtcg gcggtcgtag aggtgcgtgg cgagcccgga agcggcggag
      781 ccaactgggt catgtggttg ctcgtgctgg tctggagcgc cgacgtgggg gcctatttcg
      841 cagggcggcg attcggccgg cgcaagctgg cgccggccgt aagccccggc aagacctggg
      901 aaggggcctt tggcggcatg ctggtcagcc tcgcgctttg cggtggcgct ttgctggtgc
      961 tgggtctgcc agcctggtgg ctgctcgcgg tcgcagggct cgtcgtggtc tccatattcg
     1021 gtgacctttt cgagagcgtg atcaagcggg tgcggggcgt caaagattcg agcaacttac
     1081 ttcccggaca cggaggtatc ctggacagaa tcgactccct cctggcggtg ctgccggttt
     1141 tcgcgctgat tctcgctcgc tgatccatta tgatgcgctc gccgatggtc tttacgacgg
     1201 tcgggtaatt ccagggcggg gcgaaccggc gagcaccgct gctgtctgaa gttttgatac
     1261 ggggcatctg tcgcactcag tttatggatt ttctgcaata tccccttgcc ctgctggtga
     1321 ctctcggcgt cctggtgact ttccacgaac tgggtcattt cgtcatcgcg cgctggtcgg
     1381 gcgtcaccgt cgtccgcttc tcggtcggct tcgggaaacc tttctggtcg tggcgcgacc
     1441 ggcgcggcac cgagttcgct cttgcgtaca ttccgctggg cggctacgtg agaatgctcg
     1501 acgagcggga gcctggcgtc atcgagtacg accgccgcag cacggacgtc tcctacaacg
     1561 agctcagcgt ctggtggcgc atggccatcg ctttgggcgg gccggctgcc aatttcctgc
     1621 tggccatcat cgtctactgg gtgctctttg tgatcggtac gatcacgtac gccccgcttt
     1681 tgggcagcgt aggggcggat tccgccatcg gcagggcggg gctgcgcgag cacgaggagc
     1741 tcatcgccgt ggacggcgtc gagacgtcct cctggcaggc ggtgaacatg gcgctcgcgg
     1801 ggcggctggg cgagagcggc gagatcgagc tcactacccg gagacccgga tcggattcgg
     1861 tacgctccgg tgccgtgccg atttccagct ggcacacggg tgttgacgaa ccggatctgc
     1921 tcggctcgct cgggatcgtg cccgcactgc cccccatcgt cgcccaggtg gtgccggaca
     1981 gcccggcaga gcgcgccggg ttccgtcgct gggatcggat cgtctctgtt gatggagagg
     2041 cggtcgccga ctggggccaa tgggtggatc tgatacgcaa tgccccggac cggcgcttga
     2101 gcgtgaccgt agaccgttcc ggacaggtgc tggtgctgga cgtgacgccg gacgtccagg
     2161 aagctgccgg cggcgaggtg accggcttcc tcggggtggg cgcgtacacc aaggagatcc
     2221 ggtacggcgt gcttgggggg gatcgcctcg tttccggccg cgcgtcctct tcgcggtgtc
     2281 cacttccccg gtgatcgtcg cgggaacagg gaccttcctg gacgaactga tccgcag
//
LOCUS       DBNHKV010000457         4955 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_915247, whole genome shotgun sequence.
ACCESSION   DBNHKV010000457 DBNHKV010000000
VERSION     DBNHKV010000457.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_915247"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..806)
                     /locus_tag="AC16PP_15000"
     CDS             complement(168..806)
                     /locus_tag="AC16PP_15000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HIR6482987.1"
                     /translation="MLKLYALFGDDGAESPFQLNQAQALEVLQERFPGLRRYRQFLRT
                     GEQLGNLVLAPFRASAEFWLAEHDDPTPLMAALETAAEDVHEVFAVNPRVLIRSEEHN
                     ISGEPFHDSGFKATFLFDRKPSLSLEEFHDYWLTHHGPIAAQTQDAERYVQSHIRTAQ
                     RAYDGVTELFWPDYPTALASMASEQMTTDQANDAPNFVDGESLVVFLAREAN"
     gene            complement(810..1337)
                     /locus_tag="AC16PP_15005"
     CDS             complement(810..1337)
                     /locus_tag="AC16PP_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase light chain"
                     /protein_id="HIR6482988.1"
                     /translation="MNWLDRITQKTTRKVARATSRRGFLAGASAVLLGASATPLLPVA
                     RASESRVPPQSTGNPEDPGDPTQCDYWRYCAIDGFLCSCCGGTQNACPPGTEMSPLTW
                     IGTCRNPVDGKNYVISYNDCCGKSSCGRCLCNRNEDDRPVYRPSANNDINWCMGTTSS
                     VYNCSTAVVLGLATE"
     gene            complement(1349..2473)
                     /locus_tag="AC16PP_15010"
     CDS             complement(1349..2473)
                     /locus_tag="AC16PP_15010"
                     /inference="COORDINATES: protein motif:HMM:NF018172.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - aliphatic amine dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amine dehydrogenase large subunit"
                     /protein_id="HIR6482989.1"
                     /translation="MFASTMWVTNTVRAELQPEPVPNVLSLATPYPDTYAVVHDFAFF
                     NLIDSKFALVDIESRRFKGMLSAGQFATINLSNSRQEFYVGETLHSRGSRGQRQDVIA
                     IYDFANLDVVAEVDLPPRRANTVINLGNSRLTEDERFLLVFMQNPGTSVAVIDLDSRK
                     MVSEISTPGCALIYPTVERSFFMLCGDGRMLSITLDETGNPVSRKASEPFIDIDADPL
                     SEKSAKAGGIWHFVSYRGQVQPIDARAAQPELMTTWWLATEKERAANWRPAGWHWTAG
                     HKSGLLWVGMTPDGYDGSHKDPAPEIWLFDTGERERLARIELRAPAISISVTQHEKPY
                     LLVASTEATLDVYDAMSGEYLRSIYDLGFTPYMVHPVSYE"
     gene            2762..3079
                     /locus_tag="AC16PP_15015"
     CDS             2762..3079
                     /locus_tag="AC16PP_15015"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR6482990.1"
                     /translation="MGSFRYGFIAIAIRGVAQNDFISNQESPVSLYVDEVYISQMSGA
                     GFLLFDMERVEILRGPQGALFGRNATATSRTGCSARTSTMPTTSPCGDNCCSTSATRA
                     ACC"
     gene            2989..3303
                     /locus_tag="AC16PP_15020"
     CDS             2989..3303
                     /locus_tag="AC16PP_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6482991.1"
                     /translation="MLGKDINNADDIAVRGQLLFDLSDKGSLLLNGRYYDHDIRTGFF
                     ENFTLSSITDYQDVYRDYFEDTDASPIADLNFYLVTDAQQFSQEVRFNGETERTRWVA
                     GL"
     gene            3388..4134
                     /locus_tag="AC16PP_15025"
     CDS             3388..4134
                     /locus_tag="AC16PP_15025"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR6482992.1"
                     /translation="MIDEDGNYLGNDNPYQVDTESFSVFGQIEYDFTPQLTGIAGGAL
                     DHREEVRRLCDEFRGHVPGTTLRNGKPNIIDINPDNDTESIGTFSGSLDDDLWSAKLK
                     LDWRPNDDLLLYASYNRGVKGAGFNAPLDITVGDGVTVIPDLEQETAFKEETLNAYEI
                     GFKATLSNGLARLNGAVYCYDYEDFQAFKIMRQAGPAGGCAIPQQAPLQPDPRRGEHG
                     ERLHGGQCPRRLPIGRRSLGASGPRFQLHR"
     gene            complement(4293..>4955)
                     /locus_tag="AC16PP_15030"
     CDS             complement(4293..>4955)
                     /locus_tag="AC16PP_15030"
                     /inference="COORDINATES: protein motif:HMM:NF013717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HIR6482993.1"
                     /translation="QAMGFASAFDSGEPDMWSRIDAMLRSQGRSMMQLRAEQIIALER
                     SRPEEFYERYVQTEDGRIDCMPETFADGVDRMAAIFGELEEEPANQLKLISKRDRYML
                     NSWYSNVAKLKSGERDRNYLFMHPEDAAIRQIADGDRVRISNDNGEITSQARLTAELM
                     PGVVAMTHGWGHGRTPGMRVAQEKPGTNCNALLPSGPGSFDPLSNQAHMTGVPVEVAP
                     AG"
BASE COUNT          890 a         1512 c         1609 g          944 t
ORIGIN      
        1 gggtgcccta ctcggcgggc gaggtggccg agctgcgttc ggcaccgctc agagaagtgg
       61 agaaggtgag ggccgggctg gtccgaagca tccagcagca cctctgacct tggaggttct
      121 ggcaagcctg acccaagggg aagatggcgg ccgaaaactc gtcggtctca gttcgcctcg
      181 cgggccagga acacgaccag gctctcgcca tccacgaagt taggagcgtc gttggcctgg
      241 tcggtggtca tctgctctga agccatgctg gcgagcgccg tcgggtagtc gggccagaac
      301 agctccgtga cgccgtcgta ggcccgctgg gcggtcctga tgtggctctg cacgtagcgc
      361 tccgcgtcct gggtctgggc ggcgatgggg ccgtgatggg tcagccagta gtcgtggaac
      421 tcctccaggc tgagcgacgg cttccgatcg aacaggaagg tcgccttgaa gccgctgtca
      481 tgaaagggct cgccgctgat gttgtgctct tcgctgcgga tcagcacacg ggggttgacg
      541 gcgaagacct cgtgaacgtc ctcagcggcc gtctcgagcg ccgccatcag cggtgtcggg
      601 tcgtcgtgct ctgccagcca gaactcggcc gacgcgcgga agggggcgag caccagattg
      661 ccgagttgct cccccgtcct cagaaactgg cgatagcgac gcagccctgg aaaacgttcc
      721 tgaagaacct cgagtgcctg cgcttgattc agctgaaagg gtgattcggc cccgtcgtcg
      781 ccgaacaggg cgtagagttt gagcacgatc tactcggtgg ccaggccgag caccacggcg
      841 gtggagcagt tgtacacgct cgaggtcgtg cccatgcacc agttgatgtc gttgttggcg
      901 ctcggtcggt agacgggccg gtcgtcctcg ttacggttgc acaggcagcg tccacagctg
      961 ctcttgccgc agcagtcgtt gtaggagatc acgtagttct tgccgtccac cggattgcgg
     1021 caggtgccga tccaggtgag aggcgacatc tccgtgcccg gcgggcaggc gttctgggtg
     1081 ccgccgcagc agctgcacag gaacccgtcg atggcgcagt agcgccagta gtcgcactgg
     1141 gtgggatctc ccggatcctc ggggttgccc gtgctttggg ggggcacgcg gctctcgctg
     1201 gcccgtgcta cgggcagcaa cggggttgcc gatgcgccca gcaggaccgc tgaggcgccg
     1261 gcgagaaagc cccgccgcga agtggctcgt gcgaccttgc gcgtggtttt ctgggtgatc
     1321 cggtccagcc agttcatcgc gcggctcctt actcgtagct cacagggtgg accatgtaag
     1381 gcgtgaaacc gagatcgtag attgatcgca ggtattcgcc gctcatggcg tcgtaaacgt
     1441 ccagggtggc ctcggtgctt gccaccagaa gatagggttt ctcgtgctgg gtgacgctga
     1501 tggaaatggc gggcgcgcga agctcgatgc gggccaggcg ctcgcgctcg ccggtgtcga
     1561 agagccagat ttcgggggcc gggtccttgt ggctgccgtc gtagccgtcg ggcgtcatgc
     1621 ccacccagag caatccgctt ttgtggcctg ccgtccagtg ccagccggcc ggacgccagt
     1681 tggcggcacg ttccttctcc gtggccagcc accaggtcgt catcagctcg ggctgcgcgg
     1741 cacgggcatc gatgggctga acctgcccgc gatagctgac gaaatgccag attccgccgg
     1801 ccttggcgga cttctcggac agggggtccg cgtcgatgtc gatgaacggc tcgctggcct
     1861 ttctggaaac gggattaccg gtctcatcca gcgtgatcga gagcattctc ccgtctccgc
     1921 agagcatgaa gaagctgcgc tcgacggtcg gatagatcag cgcgcagccg ggcgtgctga
     1981 tctccgagac catcttgcgc gagtccagat cgatgaccgc caccgacgtg ccgggattct
     2041 gcatgaagac cagcagaaac cgctcgtcct cggtgagcct ggaattgccg agattgatga
     2101 cggtgttggc ccgccggggc ggcaggtcca cttcggcgac cacgtcgagg ttggcgaagt
     2161 cgtaaatggc gatcacgtcc tggcgctgac cgcggctgcc gcggctgtgc agcgtctccc
     2221 cgacgtagaa ttcctggcgg ctgttggaaa ggttgatggt ggcgaattgc ccggcgctca
     2281 gcatgccctt gaagcggcgc gactcgatgt ccacgagggc gaacttactg tcgatcaggt
     2341 tgaagaaggc gaagtcgtgt accaccgcgt aggtgtcagg gtacggggtc gccaggctga
     2401 gcacgttggg gacgggttcg ggctgaagct ccgcccgaac ggtgttggtg acccacatag
     2461 ttgaagcaaa catcacgcac gtgacatgaa cagagaagac tcgcgcccaa gttcttcgaa
     2521 tcattggtct ccccccgaat cctggcgtcg gatactcgac atgcaccatc aagacacgag
     2581 atgcgtcgag ccggacgcta ctcggcccgc acgaggctgt caacggcgcc gaacagtctg
     2641 atccccgcct ttcaacggcc tcgccgcagc ccgcatttgt gttgaattcc ggcctgatgg
     2701 ggccgccgag gtggagcagt caggtcgcga gcctgattgc gcggctggcc cagcaattta
     2761 gttgggtagt tttcgttatg gtttcattgc catcgccatt cgcggtgtcg cgcagaacga
     2821 cttcatcagc aatcaggaga gccccgtttc tctgtacgtc gatgaggttt acatcagcca
     2881 gatgtctggc gccggcttcc tgctgttcga catggagcga gtggagatct tgagaggtcc
     2941 tcagggcgcc ctgttcgggc gcaacgcgac ggctacatcg agaaccgggt gctcggcaag
     3001 gacatcaaca atgccgacga catcgccgtg cggggacaat tgctgttcga cctcagcgac
     3061 aagggcagcc tgctgctgaa cggccgctac tacgatcatg acatccgcac gggattcttc
     3121 gaaaacttca cgctgagctc catcaccgac tatcaggacg tctatcgcga ttattttgaa
     3181 gacaccgatg cctcgcctat cgccgacctc aatttctatc tcgtgacgga cgcccagcag
     3241 ttctctcagg aggtccgctt caacggcgag accgagcgaa cccgttgggt ggctgggctc
     3301 tagtacctgg acatcgatat tgaggatgcc aacggcgcgg agacgccgct gacggtctta
     3361 gcggacgacc cgtcaggctt cttcccgttg atcgacgagg atggcaacta cctcggaaac
     3421 gacaatccgt atcaggtcga caccgaatcc ttctcggtgt tcggccagat cgagtacgac
     3481 ttcacccctc agctcacggg catcgccggg ggtgcgctgg atcatcgaga ggaagtccga
     3541 agattatgcg atgaatttcg tggccacgtg ccgggaacca ccctccgcaa tggcaaaccg
     3601 aatatcatcg acatcaaccc ggacaacgac acggagtcca tcggcacgtt ctcgggctcg
     3661 ctcgatgacg acctctggtc ggccaagctg aagcttgact ggcggcccaa cgacgatctg
     3721 ctcctgtacg ccagctacaa ccgtggggtg aaaggcgccg ggttcaacgc gcccctggac
     3781 atcaccgtcg gtgatggtgt gactgtgatc cctgacctcg agcaagagac ggcgttcaag
     3841 gaagagaccc tgaacgccta cgagatcgga ttcaaggcga ccttgagcaa cgggctggcc
     3901 cggctgaatg gcgccgtcta ctgctacgac tacgaagact tccaggcctt caagatcatg
     3961 cggcaagctg gccctgcagg cggatgcgca ataccgcagc aagcaccact tcagcctgac
     4021 ccgcgccgag gcgagcacgg agaacggcta cacggtggcc aatgcccgcg tcgcttaccg
     4081 atcgggcgac gatcgctggg agctagcggc ccacgtttcc aacttcaccg atgaggaata
     4141 cctggtgcag accttcgatc tcgccggcgt gctcggcatg acggagcagt actacggcat
     4201 gcccgggtgg tggtgggtaa cggcttcgta ccagttcgga aactgaccgg gctgtccggc
     4261 aacggcagcc ccgcgcgctg agaggtcgcc gctcacccgg cgggcgctac ctccacgggc
     4321 acgcccgtca tgtgggcctg gttcgacagt gggtcgaagc tgccggggcc tgacggtaac
     4381 agggcattgc agttggtgcc cggtttctcc tgagcgacac gcatgccggg ggtgcggccg
     4441 tgaccccagc cgtgggtcat ggccaccacg ccgggcatga gctcggcagt caatctggcc
     4501 tggctcgtaa tctcgccgtt gtcgttgctg attcggaccc gatccccgtc cgcgatctgg
     4561 cggatggcag cgtcctcggg atgcatgaag aggtagttcc gatcccgctc gcccgacttg
     4621 agcttggcca cgttgctgta ccagctgttg agcatgtacc ggtccctttt gctgatcagc
     4681 ttcagctggt tggccggttc ctcctcgagc tcgccgaaga tggcagccat acggtcgacg
     4741 ccatcggcaa acgtttcggg catgcagtcg atccgaccgt cctccgtctg cacgtagcgc
     4801 tcgtaaaact cttcaggcct cgaccgctcg agagcgatga tctgctcggc gcgcagctgc
     4861 atcatgctgc gtccctggga ccgcagcatg gcgtcgatcc gtgaccacat gtcgggctcg
     4921 ccgctgtcga aggccgaagc gaaccccatg gcctg
//
LOCUS       DBNHKV010000458         9843 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_773710, whole genome shotgun sequence.
ACCESSION   DBNHKV010000458 DBNHKV010000000
VERSION     DBNHKV010000458.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9843
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_773710"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="AC16PP_15035"
     CDS             complement(<1..758)
                     /locus_tag="AC16PP_15035"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6482994.1"
                     /translation="MSAVLDTLGERFAAYLTKSWKQPATTLHIEQIPGGASRETYRVR
                     VSIGGQEQGLILRRDPVSSLIDTERSLEYRTYEAVFPTSIPVPEPLLLEEDAVHLERP
                     FSVMREIGGCESSAQSLAAPPYQAMRETIGRRKWEILGQLASLDLSELGVDAFMAVPD
                     DPARRELEHWSKVIADDALHPQPIAEAARRWLERNLPEPSGRFALVHGDYRSGNFLYD
                     EEGVIRGVLDWEMAHIGDPLEDLAWSLDPLWSWP"
     gene            complement(778..2118)
                     /locus_tag="AC16PP_15040"
     CDS             complement(778..2118)
                     /locus_tag="AC16PP_15040"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6482995.1"
                     /translation="MAEAYEFHDDDVIAPEVYGRHGHPHGAFAWLRANDPLRYVEPTE
                     YLPFWAVTKHADIVEIEKQPEIFASEPRPILVKEAQSPEVRQDVLMQIFERLKDSPKL
                     LEVLATIEQGGLIRSLVQMDPPDHPKYRTLVQPWFKPSNIKRLEDSLTRIIANILDEM
                     MGDGSERELEFVQDVAVWPPLKLITELLGVPEEDEWRILKLTNELFAGDDPEMKRAGD
                     DPLSIIDTIKDLYDYFTRLTDERRANPTEDLSSYIANGRIDGEYLPYKELISYYIIVA
                     TAGHETTRTAISGGLLALLENPEQMSLLRNNIDDEDLIKLAVEEMIRWTTPVSQFSRT
                     AMRDYELRGKQIRQGDALGLFYASANRDEEIFDDPFNFRVDRQPNRHLAFGTGPHICL
                     GILLARLEMRIFFKQFLPRIEKMELVDRGPEYLKASFVHGLKHLPIRFQLRPAR"
     gene            2263..3039
                     /locus_tag="AC16PP_15045"
     CDS             2263..3039
                     /locus_tag="AC16PP_15045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6482996.1"
                     /translation="MKLSKLSRSVEGKVVIVTGAASGMGRATARVFADEGAKVAAMDI
                     NAEPLNEVVDEIAAAGRDIKGWALDLSDPDAIVEVFAEVPDHFGGIDILVNNAGISMF
                     APIDSDDYEHAWERSLTVLLTAHTRTIRAALPYLRKAEAARIINIASTEGLGATKFGS
                     PYTAAKTGVIGLTRSLAVELGEEGITVNCICPGPILTGMTAAIPDEAKAEFARRRVAL
                     KRYADPEEVAHATLSLALPAACFITGVALPVDGGLTIRNA"
     gene            3057..4472
                     /locus_tag="AC16PP_15050"
     CDS             3057..4472
                     /locus_tag="AC16PP_15050"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6482997.1"
                     /translation="MADGSPTPYADPRARVPNTAVVAYGAPGIGAGYMYLMMSLYVMK
                     FSTDVLLIAPAVMGVIFSVSRIWDAVSDPLAGYLSDRTRSRMGRRRTWILGSMIPIGG
                     AFIMVFAPPDGLSDGALVAWMAVAIIGFYSAMTGFLVPHLSLGAELSESYHERSRLFG
                     VRHGCFTFGSILSLVSFYFLISAEQQGAEEVRNTAFWLAVLAGGVMCLLMAFGVWKLR
                     ERADYQGRVNENPIQAFADVWRNRHARIIIAVSFIENVGSAAIGALTLYVAQYVVGAP
                     MWAPAIILCYMIPSTFSVPLWIPLSRRFGKIRLWIFSLVLTGVAFGSMFVLPFMESMP
                     LKLAYIATAAVFAGFAAGCGGTVSPSIQGDVIDYDEYLTGERKEGSYFAAWNFVYKSA
                     TGIMLLLTGFVLQASGFVPNQEQTMTVQLAMITLYSLFPLVCYSIGSLLLKRFDLDED
                     AHGEIRRALDERNAGRAASAP"
     gene            complement(4459..5244)
                     /locus_tag="AC16PP_15055"
     CDS             complement(4459..5244)
                     /locus_tag="AC16PP_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6482998.1"
                     /translation="MTRFGDIAVETTGHVAVLEIQRPPNNFFDVDLINAMGDAFEAMD
                     EDANVRALVLAADGKHFCAGANFGNRDEQSRRQARTATDGNPLYSAAVRLFACRKPVI
                     AAVQGAAVGGGFGLAVMPDFRVVCPETRFTANFVKLGFHPGFGLTYTLPRLIGQQRAN
                     LMFMTGRRIDGETACEWGLADILTDRDSLRARAMELAQEIAENAPLAVTSVRATLREG
                     VAEAVKRQTDHEFIEQHRLQQTEDHKEGVRAVAERRPGNFKGR"
     gene            5385..6227
                     /locus_tag="AC16PP_15060"
     CDS             5385..6227
                     /locus_tag="AC16PP_15060"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6482999.1"
                     /translation="MLKWQIGEVSVTQIVELSTASLGPYLLPQADPGALLEVPWLRPF
                     IDDDGRIVLSVHALVVESEGERLIVDTCIGNDKERTYPRWNRMQTDFLDRLAQAGYPP
                     ETIDTVMCTHMHVDHVGWNTRLTDDGWVPTFTRARYLFAEDEWAYWQREPQEFGPVVE
                     DSVQPIFDAGLAEVVGQTHRVTDEVWLEPTPGHTPGHVSVHIASAGEEAIITGDMIHH
                     PCQIAHPEWSSSADWNQEASAETRDAFLERYADRPVLVIGTHFAAPTAGHIVRDGESF
                     RLDY"
     gene            6330..7598
                     /locus_tag="AC16PP_15065"
     CDS             6330..7598
                     /locus_tag="AC16PP_15065"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6483000.1"
                     /translation="MSSRNIYQALEVPDFRWLWVGSLASSFAMNMQIVARGWLVYTLT
                     SSALDLAWVTLSFMVPMVAFSLWGGVVADRLPKRTIIAIAQALNTLATIVMGVIIFSD
                     LVSFWDFVWFGFFNGTVLALSMPARQAFAPELLPDRLIFTAMALNTTSWNLSRILGPA
                     FAGFLIAWVAAGDTSSTYGVGIVYVVIAALYFLSMATVLLVRKPGIVSSREQRKSPLT
                     DMADAFRYVWANPPVFGLIVLSIVPFLFGMPINTLLPAFNEDILGGGPDDLGLLMSTM
                     GMGAIVGSLLLAGVNDFRHRAAWLIATCVGWGAFTCAFGFSQTLMLSAGLLAVIGFLS
                     SWNMSLNRGLLQLQVDDHMRGRILSVDMMSHGLMPLGVIPISLIAEQFSVATALVVSG
                     AIFIVVVLLLLVVSPSIKRVDDDLQPVSGG"
     gene            7595..8194
                     /locus_tag="AC16PP_15070"
     CDS             7595..8194
                     /locus_tag="AC16PP_15070"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6483001.1"
                     /translation="MSVDVRIGLISDTHIPEARKTLWPQVFTAFDGVDMIMHAGDIHE
                     LYVLDALEEVAPVVAARGNGEDGGGGRPVQPDDPRVKYAWLLELGGLWVGLTHYVPVP
                     ERPPQFTLARWVERYFPERTPDIIVSGDTHREIIAEVQGVLCVNPGSPTYPHNYDTQY
                     GTLGFLDLVDGEARPSVWQITDGGIEPFDWDAVPPWKRR"
     gene            complement(8221..9381)
                     /locus_tag="AC16PP_15075"
     CDS             complement(8221..9381)
                     /locus_tag="AC16PP_15075"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6483002.1"
                     /translation="MDFEYTADEQAFREEVRDFLATNLPPREERGKDFLASWLTNVRE
                     KGWVGFSWPKEFGGSDGGLVEQAILREEMAVAKAPPLGTSFMGLAWVGPGIIQYGTED
                     QKRRFIPDILDSKVIWCTGYSEPNHGSDLAAIQCKAVREGDHYVVNGQKIWTSGAPWS
                     QWIILLVRTDFDVDVKHKGITCLLVPMDSPGVEVKPIENMAGDRHFAEVFFTDVKVPL
                     ENRLGDEGDGWKVTVSALANERSSIGEVTQMFGKLDTLKELVRTTTRQGRPASEDPKV
                     RRTIAMFETKIAAMKYNGLRYLTKQIKGEPLSSETSVNKLHRASLEIEMDDFAVELTG
                     QAGLLLSGSEEAVSGGSWAKSSLSWPNVVIGGGTPNIQRNIIAERILGQPKD"
     gene            complement(9381..>9843)
                     /locus_tag="AC16PP_15080"
     CDS             complement(9381..>9843)
                     /locus_tag="AC16PP_15080"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6483003.1"
                     /translation="LPLTDADLAFLTDCGAVAVTAEMVGAAEQTLAMTSEYAKERIQF
                     GKPIGQYQGVKHRLADIYVDVESFKSLLYYAAWAVDEAPDELPRAISMAKGYASDAFA
                     RIGIDGIGLHGAIGFTAEYDIQLYLKRSKWARPMYGDSDYHLDRVATLGGL"
BASE COUNT         1572 a         3128 c         3205 g         1938 t
ORIGIN      
        1 tcgggccatg accacagggg atcgagggac catgccagat cctccagggg atcgccgatg
       61 tgggccatct cccagtccag cacgccccga atgacgcctt cttcatcgta gaggaagttg
      121 cccgagcgat agtcgccgtg gaccagggca aagcggcccg acggctcggg cagatttcgc
      181 tccagccagc ggcgggcagc ttccgcgatg ggctggggat gcagcgcgtc gtcggcaatg
      241 accttcgacc agtgctcgag ctcccgtcgt gcaggatcgt ccggcaccgc catgaaggcg
      301 tcgacgccca gctcgcttaa gtcgagcgac gcgagctggc cgagaatttc ccactttcgc
      361 cggccgatgg tctcgcgcat ggcctggtag ggcggtgctg cgagggactg cgcgctggac
      421 tcgcagccgc cgatctcgcg catcacggaa aacggccgtt ccaggtggac ggcgtcctcc
      481 tcgagcagca gcggctcagg cacggggatc gacgtcggaa atactgcctc gtaggttcgg
      541 tactcgaggg aacgctcagt gtcgatcagg ctggaaacgg gatcgcgccg aagtatgagt
      601 ccttgctctt gcccaccgat tgaaaccctg acgcggtagg tttccctgga agcgccgccg
      661 ggaatctgct cgatgtgcag cgtcgttgcc ggctgcttcc agctctttgt caggtaagcg
      721 gcaaagcgct cgcccagggt atcgagaacc gcgctcatga cccggttgcc cgtgtgctca
      781 ccgcgccggt ctcagctgga accgaatcgg caggtgcttg agcccgtgca cgaagcttgc
      841 cttgaggtac tccggacccc ggtcgaccag ttccatcttc tcgatgcggg gcaggaactg
      901 cttgaagaat atccgcatct ccagccgcgc cagcaggatg ccgagacaga tgtgaggccc
      961 ggtcccgaag gcgaggtgac ggttgggctg gcggtcgacc ctgaaattga aggggtcgtc
     1021 gaagatttcc tcgtcccggt tggcggaggc gtagaacagg cccagcgcgt cgccctggcg
     1081 aatctgcttg ccgcgcagct cgtagtcccg catggcggtg cgggagaact gactgaccgg
     1141 cgtcgtccag cggatcatct cctccacagc gagcttgatc aggtcctcgt cgtcgatgtt
     1201 gttccgcagc agactcatct gctccgggtt ttcgagcagg gcgagcagac caccggaaat
     1261 ggccgtgcgc gtggtctcgt ggccggccgt cgcgacgatg atgtagtagg agatcagctc
     1321 cttgtacggc aggtactcgc cgtcgatgcg gccgttggcg atgtacgagg aaaggtcctc
     1381 cgtggggttg gcgcgccgtt cgtcagtcaa gcgggtgaag tagtcgtaca ggtccttgat
     1441 ggtgtcgatg atggagagcg gatcgtcgcc tgcccgtttc atctccgggt cgtcgccggc
     1501 aaacagctcg ttggtgagct tgaggatccg ccactcgtcc tcctcgggca cgcccagcag
     1561 ctcggtgatc agcttgagcg gcggccagac ggcaacgtcc tggacgaact ccagctcgcg
     1621 ctcggagccg tcgcccatca tctcgtcgag tatgtttgcg atgatgcggg tcaagctgtc
     1681 ctcgaggcgt ttgatgttgg atggcttgaa ccagggttgg accagcgtcc ggtacttcgg
     1741 gtggtctggg ggatccatct ggaccagcga gcggatcaac ccgccctgct cgatggtggc
     1801 cagcacctcc aggagcttcg gcgagtcctt gagcctctcg aagatctgca tcaggacatc
     1861 ctggcgcacc tccgggctct gtgcctcctt gaccaggatc ggccttggtt cgcttgcgaa
     1921 gatctccggc tgcttctcga tctcgacgat gtcggcgtgc ttggtcacgg cccagaaggg
     1981 gaggtactcg gtgggctcga cgtagcgcag cgggtcgttg gcgcggagcc aggcaaacgc
     2041 gccatgggga tggccgtggc gtccgtaaac ctccggagcg atcacgtcgt cgtcgtggaa
     2101 ttcgtaggct tctgccatct tcgagggaac tcccgccggt gggtgaagtg gtttacgtta
     2161 gcgggattcc ccggccgatt gcaatgtgcg atggtgctcc ctagagtggc acgggaccgc
     2221 atcgaacggg cagaccgctc cggccgtcac gaggtacacg atatgaagct cagcaagctc
     2281 agccgatcgg tggaaggcaa ggtggtcatc gtcaccggtg cggccagcgg tatggggcgc
     2341 gccacggcgc gggtctttgc cgacgagggc gccaaggtcg cggccatgga catcaacgct
     2401 gagccgctca acgaggttgt cgacgagatc gctgccgcgg gccgtgacat caagggctgg
     2461 gcgctcgatc tttccgaccc cgacgccatt gtcgaggtgt tcgcagaggt gcccgatcat
     2521 ttcggcggca tcgacattct ggtgaacaac gcgggcatat caatgttcgc gcccatcgac
     2581 agcgatgatt acgagcacgc ctgggaacga tccttgaccg tgctcctcac cgcgcatact
     2641 cgaacgatcc gcgcggcgct cccttatctg cgcaaggccg aggctgccag gatcatcaac
     2701 atcgcttcca ccgaggggct cggcgcgacg aagttcggca gcccttacac ggcggcgaag
     2761 acgggcgtca tcgggttgac gcgctccctg gcggtggagc ttggtgagga aggcatcacc
     2821 gtgaactgta tctgtccggg accgatcctc acgggcatga cggctgccat tcccgacgaa
     2881 gccaaggccg agttcgcgcg acgccgggtg gccctcaagc gctatgcgga tcccgaagag
     2941 gtcgcccacg ccacgttgag cctggcgttg ccggcagcct gcttcatcac gggcgtagcc
     3001 ctgccggtgg atggcggtct gaccatccgc aacgcctgag ccgcgggcgg ggtcgggtgg
     3061 cagacggctc gccgacgccg tacgcggacc cccgcgctcg cgtgcccaac accgccgtgg
     3121 tcgcctacgg cgcgcccggt atcggggctg ggtacatgta cctgatgatg agcctttacg
     3181 tcatgaagtt ctccacggac gtgctgctca tcgcgccggc cgtcatgggc gtcatcttca
     3241 gcgtatcgcg tatctgggac gcggtatccg atccgctggc cggctacctc agcgatcgca
     3301 cgcgcagccg catgggccga aggcgaacct ggattctggg cagcatgatt cccatcggcg
     3361 gtgccttcat catggtcttc gcgccgccgg acggcctcag cgacggcgcc ctggtggcct
     3421 ggatggccgt tgccatcatc ggtttctact ccgcaatgac cggattcctg gtgccgcacc
     3481 tttcgctggg tgccgagttg tccgagagct accacgagcg gagccgactg ttcggcgttc
     3541 gccacggctg cttcaccttc ggctcgattc tctctctcgt cagcttctac tttctgatca
     3601 gcgctgagca gcagggagcc gaggaggtca gaaacacggc cttctggctg gcggttctgg
     3661 ccggcggcgt tatgtgcctg ctcatggcct tcggtgtctg gaagctgagg gagcgcgccg
     3721 actaccaggg aagggtcaac gagaacccga ttcaggcatt tgccgacgtt tggcgcaacc
     3781 ggcatgcccg catcatcatt gccgtgtctt tcatcgagaa cgtcggctcc gcggccatcg
     3841 gggcgctgac actttacgtc gcccagtacg tggtcggcgc gcccatgtgg gcccctgcca
     3901 tcatcctctg ctacatgatt ccttccacct tctcggtgcc cctttggatt ccgctttcga
     3961 gacggttcgg caagatcagg ctctggatct tctcgctggt gctcaccggt gtcgcgtttg
     4021 gcagcatgtt cgtgctcccg ttcatggaga gcatgcccct caagctggcc tacatcgcca
     4081 ccgccgccgt ctttgccggc ttcgctgccg gctgcggtgg gacggtgtcg ccgtccatcc
     4141 agggtgacgt catcgactat gacgagtatc tgacggggga acgaaaggag ggctcgtact
     4201 ttgcggcctg gaatttcgtg tacaagagcg cgacgggcat catgcttctg ctcacgggat
     4261 tcgtgctgca ggcatcggga ttcgtgccca atcaggaaca gaccatgacg gttcagctgg
     4321 ccatgatcac gctgtacagc ctgtttccgc tggtctgcta cagcattggc tcgctgcttc
     4381 tcaaacgctt cgatctcgac gaggacgccc acggagagat ccgtcgcgcc ctggacgagc
     4441 ggaacgccgg cagggccgct agcgcccctt gaaattgccc ggccgtcgtt cggccacggc
     4501 gcgcacgcct tccttgtggt cctcggtctg ctgcaggcga tgctgctcga tgaactcgtg
     4561 gtcggtctgg cgcttcacgg cttcggcgac gccctcacgc agggttgccc gaaccgaggt
     4621 aaccgcgagc ggcgcgttct ccgcaatttc ctgagcgagc tccatggctc gggcacggag
     4681 ggagtcccgg tccgtcagaa tgtcggccag gccccactcg caggcggttt cgccgtcgat
     4741 gcgccggccc gtcatgaaca tcagattggc gcgctgctgg ccgatgagcc ggggcagggt
     4801 gtaggtgagg ccgaagcccg ggtggaagcc gagctttacg aagttggcgg tgaaccgggt
     4861 ctcgggacag acgacccgaa agtccggcat gacggcaagg ccgaaaccgc ccccgacggc
     4921 ggctccctgc accgcggcga tcaccggctt cctgcaggcg aacagccgaa cggcagccga
     4981 gtagagcggg ttgccgtcgg tggccgtgcg cgcctggcgg cgggactgct cgtcccggtt
     5041 gccgaagttg gcgcccgcgc agaagtgctt gccgtcggcg gccaggacca gcgcccggac
     5101 gttggcatcc tcgtccatgg cctcgaaggc atcccccatg gcgttgatca ggtccacgtc
     5161 gaagaagttg ttgggcggac gctgtatttc cagcaccgcc acgtgtccgg tggtctccac
     5221 cgcgatgtcg ccgaatctgg tcatgatctc tcccccgggg tatgtagctg acgagcgaca
     5281 ttatgacatg gcatgccgcg cccaaggccg gggaacggcg ggatttgacc gccgtcagcc
     5341 gatctgcaag gctcgccggg tcggggtgag gtcaggggga ttccatgctg aaatggcaga
     5401 ttggtgaggt ttcggtcacg cagatcgtgg agctctccac agcctcgctc ggcccgtacc
     5461 tgctgcccca ggccgatccg ggtgcgcttc tcgaggtacc ctggcttcgg ccgttcatcg
     5521 acgacgacgg ccggatagtg ttgagcgtcc atgccctcgt ggtggagtcc gaaggggagc
     5581 ggctgatcgt cgatacctgc ataggcaacg acaaggagcg cacctacccg cgctggaacc
     5641 gcatgcagac cgattttctg gatcggctcg ctcaggccgg gtatcccccg gagaccatag
     5701 acacggtgat gtgcacgcac atgcacgtcg atcacgttgg ctggaacacg cggctgacgg
     5761 acgatgggtg ggtacccact ttcacccggg cgcgctatct cttcgccgag gacgagtggg
     5821 cctactggca gcgcgagccg caggagttcg gccccgtggt ggaggactcg gtgcagccga
     5881 tcttcgatgc cgggctcgcc gaggtggtgg ggcagaccca tcgggtcacg gacgaggtct
     5941 ggctcgagcc gacgcccggg cacacgcccg gtcacgtgag cgtgcacatt gcttccgccg
     6001 gcgaggaagc catcatcacg ggggacatga tccatcatcc ctgccagatc gctcatccgg
     6061 aatggtcgag cagcgcggac tggaatcagg aggccagcgc cgagacgcgc gatgcgtttc
     6121 tcgagcgata tgccgaccgt cccgtgctcg tgatcgggac ccattttgcc gcgccgactg
     6181 ccgggcacat cgtgcgtgac ggggaaagct tccggctcga ctactgattt tattgcccgg
     6241 ccggccctcc cgcggggttc gggctgggac ggtgacgccc cgcggctgtg ctaaagtgac
     6301 gggcttgccc gaccctaccg gatccgccgt tgtcgtcacg aaacatctac caagctctgg
     6361 aagtgcccga cttccgctgg ctttgggtgg gcagcctggc ttcgtctttc gccatgaaca
     6421 tgcagatcgt cgcccggggc tggctggtct acacgttgac gtcgtcggct cttgacctgg
     6481 cctgggtgac cctgtcgttc atggtgccca tggtggcctt ctcgctgtgg ggtggcgtgg
     6541 tggccgaccg gctcccgaag cgcaccatca tcgccatcgc gcaggcgttg aacaccctgg
     6601 cgaccatcgt catgggtgtg atcatcttct ccgatctggt gtctttctgg gatttcgtct
     6661 ggttcggttt cttcaatgga accgttctcg cgctttccat gcccgcccgc caggcgttcg
     6721 cgcccgagct cctgccggat aggctgatct tcacggccat ggcgctgaac accaccagct
     6781 ggaacctctc acgcattctc ggccccgcgt ttgcaggctt tctcatcgcg tgggtcgcgg
     6841 cgggcgacac gtcctccacc tacggggtag gcatcgtcta cgtcgtgatt gccgcgctct
     6901 acttcctttc catggcgacc gttctgctgg tacgcaagcc cggcatcgtg agttcccgcg
     6961 agcagcgcaa gtccccgctc accgatatgg cggacgcctt tcgttacgtc tgggccaacc
     7021 cgccggtatt cggcctgatc gttctctcca tcgtgccgtt tctgttcggc atgcccatca
     7081 acaccctgct gccggcgttc aacgaggaca ttctgggcgg ggggccggac gatctggggc
     7141 tgctgatgtc gaccatgggc atgggcgcca tcgtcggatc cctgctgctg gccggcgtga
     7201 acgacttccg gcaccgcgcg gcctggctca ttgctacctg cgtaggctgg ggggccttca
     7261 cctgcgcgtt cggcttctcc cagacactga tgctctccgc cgggctgctc gcggtgatcg
     7321 gctttctgag ctcctggaac atgtcgctca accgcgggct gctccagctt caggtggacg
     7381 accacatgcg ggggcggata ctcagcgtcg acatgatgtc ccacgggctc atgccgctgg
     7441 gcgtcattcc catcagcctc attgccgaac agttcagcgt ggccacggcg ctggtggtca
     7501 gcggagcgat cttcatcgtg gtggtgctgt tgcttctggt ggtgtcccct tcgatcaagc
     7561 gggtggacga tgacctgcag cccgtctcgg gcgggtgagc gtcgacgtgc gcatcggcct
     7621 gatatcagac acacacattc cggaggcgcg caaaaccctc tggccgcagg tcttcaccgc
     7681 cttcgacggc gtcgacatga tcatgcacgc gggggacatt cacgagctgt acgtcctgga
     7741 tgccctggag gaggtagccc cggtggttgc cgcccggggc aacggggagg atggtggcgg
     7801 cggtcgaccc gtgcagccgg atgatccgcg cgtgaagtat gcctggctgc tggaactcgg
     7861 gggtctctgg gtgggcctga ctcattacgt gccggtgccc gaacgcccgc cacagttcac
     7921 tctggcgagg tgggttgaac ggtactttcc cgagcggacg cccgatatca tcgtctcggg
     7981 ggacacccac cgggagatca tcgcggaggt gcagggcgtg ctctgcgtga atccgggctc
     8041 gcccacctat ccccacaact acgataccca gtacggcacg ctggggtttc tcgacctcgt
     8101 cgacggggag gcaaggccca gcgtctggca gatcaccgac ggcggcatcg agcccttcga
     8161 ctgggacgcc gtgccgccct ggaagcgtcg ctgaccggcg ggcccgttgg cccggctagg
     8221 tcagtccttg ggctgcccga ggatgcgctc ggcgatgatg ttgcgctgga tattgggcgt
     8281 gccgccgccg atcaccacgt tcggccagga aaggctcgac tttgcccagc ttcctcctga
     8341 cacggcttcc tcgctgccgg agagcaggag cccggcctga ccggtgagct ccaccgcgaa
     8401 gtcgtccatc tcgatctcga ggcttgcgcg atgcagcttg ttcaccgatg tctcgctgga
     8461 caacggctcg cccttgatct gcttggtcag gtaacgcaaa ccgttgtact tcatcgcggc
     8521 gatcttggtt tcgaacatcg cgatcgtccg cctgaccttg ggatcctcgc ttgcgggccg
     8581 tccctgcctg gtagtggtcc tgacgagttc tttgagcgtg tcgagcttgc cgaacatctg
     8641 ggtgacctcg ccgatgctgg agcgctcatt ggcaagggcg gacacggtga ccttccagcc
     8701 gtctccctcg tcgccaagac ggttctccag cggaaccttc acgtcggtaa agaagacctc
     8761 ggcgaaatgc cggtcaccgg ccatgttctc gatgggcttg acctcgacgc ctggagaatc
     8821 catcggtacc agcaggcacg tgatgccctt gtgcttcacg tccacgtcga agtccgtccg
     8881 taccaggagg atgatccatt gggaccacgg cgcgccgctg gtccagatct tctgaccgtt
     8941 gacgacgtag tggtcgccct cacgcacggc cttgcactga atggcggcaa ggtcggaccc
     9001 atggttcggc tcggagtagc ccgtgcacca gatgaccttg ctgtccagga tgtcgggaat
     9061 gaagcggcgt ttctgatcct ccgtcccgta ctggatgatg ccggggccca cccaggccag
     9121 acccatgaac gaggtgccga gcggcggggc cttggccacg gccatctctt cccggaggat
     9181 ggcctgttcc accaacccgc cgtcgctccc gccgaactcc ttcggccagg aaaacccgac
     9241 ccatcccttc tctctgacgt tcgtcagcca gctggcgagg aagtccttgc cgcgttcttc
     9301 ccggggcggc aggttggtcg ccagaaaatc gcgaacctct tccctgaacg cctgctcgtc
     9361 ggccgtgtac tcgaaatcca ctacaaacct cccagggttg cgacccgatc caggtgatag
     9421 tcggagtccc cgtacatggg gcgcgcccac ttcgaccgtt tgaggtaaag ctgaatgtcg
     9481 tactcggcgg tgaagccgat ggcgccgtgc aggccgatgc cgtcaatgcc gattctggca
     9541 aaggcgtcgg aggcgtaacc ctttgccatg gagatggccc ggggcaactc gtcgggcgct
     9601 tcgtcgacgg cccaggcggc gtagtacagc agcgacttga acgactcgac gtcgacgtat
     9661 atgtccgcca gtcggtgctt gacgccctga tactgcccga ttggcttgcc gaactggatg
     9721 cgctccttgg cgtactcgct ggtcatcgcg agcgtctgct cggcggcgcc gaccatttcc
     9781 gcagtgaccg cgacggcgcc gcagtcggtc agaaacgcca ggtccgcatc ggtgaggggc
     9841 agc
//
LOCUS       DBNHKV010000459         2346 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1197637, whole genome shotgun sequence.
ACCESSION   DBNHKV010000459 DBNHKV010000000
VERSION     DBNHKV010000459.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1197637"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="AC16PP_15085"
     CDS             <1..307
                     /locus_tag="AC16PP_15085"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6483004.1"
                     /translation="PAYTTAKHGVIGLTRQAALTYVNQGVRVNAVSPGPSVSAVSEHL
                     QQQPAEVVQAMLASLNPTGQLVPAEEIARTVAFLCSDQALMINGHAIPLDGGQLAKL"
     gene            382..1707
                     /locus_tag="AC16PP_15090"
     CDS             382..1707
                     /locus_tag="AC16PP_15090"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6483005.1"
                     /translation="MAEARVTTNSTQPPRTPITAERYISREYMAREWEKIWSKMWLVA
                     GVEQDVAEPGDYFVFNLEPESIIVSRTSDGELAAFYNVCQHRGAKVVLSDLGALEQYT
                     CPYHGWTYSNDGKLVHVPDEDRFSQGVPKAELSLKPLRVEAWAGVIWVCMDPDAPTLD
                     EFLGPIKAMVEPFRTREMRLIEDQSVRLACNWKAVFDNFGELYHVEHIHPQHETIFDC
                     PASTAELFDKGHTRVLIDGFTVNTRLPIPDEVPMTQWAQMEALGMNKDDYRGRVLDVR
                     RDIQIKKREMAPVLGYNYDTLTDEQLSDIVQYNVFPNAILVIQPEELWILRSRPRGSD
                     PDKCYWDKLALRMFPDRQAQKRANISFNLDQEGVPTGNARPEHDEFEQEDIIAGRKTM
                     TITLDQDIHLIRDVQKGMHSRGFGEAWLNDDESRVQHFHAWLDRYMAGG"
     gene            complement(1770..>2346)
                     /locus_tag="AC16PP_15095"
     CDS             complement(1770..>2346)
                     /locus_tag="AC16PP_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6483006.1"
                     /translation="AHNWGFEGNMIYQANDRLQFRLAGQFNHLEFDEWEDAGCHPVDG
                     ALGDDLPTQGPGCHIGPDGVAIQDLSGVRYGGPPFQANVGFTYDMPLMRDWRLTIDWD
                     TIHHNKGKRTLNQPFTEIPSRTVTNIGATLSQDSGNWEAGVICTNCFNDLYVYAISNR
                     PLAKIIPGERGDLYGQVAPPRLVSLYLTYRM"
BASE COUNT          451 a          771 c          748 g          376 t
ORIGIN      
        1 gcccgcctac accaccgcca agcacggcgt catcgggctg acccgccagg cggccctgac
       61 gtacgtgaat cagggcgtcc gggtgaacgc cgtctcgccg gggcccagcg tgagcgcggt
      121 gagcgagcac ctgcagcagc agccggccga ggtggtgcag gccatgctcg cctctttgaa
      181 ccccaccggg cagctggtgc ccgccgagga gatcgcccgg accgtggcgt ttctctgctc
      241 ggatcaggcg ctgatgatca acgggcacgc catccccctg gacggcgggc agctggccaa
      301 gctctgattg ccgccgcccg gcgttgcgga cactctccca aggtgttaca caggccgcag
      361 ggaaacaaag ggagcaacgc catggcagag gcaagagtta ccaccaacag cacgcagccg
      421 ccacgtacgc ccatcaccgc cgagcgctac atctcgcgcg agtacatggc ccgggagtgg
      481 gagaagatct ggtcgaagat gtggctggtg gccggcgtgg agcaggacgt ggccgagccc
      541 ggagattact tcgtcttcaa cctcgagccc gagtccatca tcgtctcccg gaccagcgac
      601 ggtgagctcg ccgcctttta caacgtctgc cagcaccgcg gtgcgaaggt ggtgctctcg
      661 gatctcggcg cgctggagca gtacacgtgc ccctaccacg gctggacgta cagcaacgac
      721 ggaaagctcg tccacgtgcc ggacgaggac cgcttcagtc agggggttcc caaggccgag
      781 ctttcgctga agccgctcag ggtggaagcc tgggccggcg tcatctgggt ctgcatggac
      841 ccggacgcgc ccacgctgga cgagttcctc ggccccatca aggccatggt ggagccgttc
      901 cgcacccggg agatgcgcct gatagaggac cagtcggtgc gcctggcgtg caactggaag
      961 gccgtgttcg acaacttcgg cgagctctac cacgtggagc acattcaccc ccagcacgag
     1021 accatcttcg actgcccggc ctccacggcc gagctgttcg ataagggcca tacgcgggtg
     1081 ctcatcgacg gcttcacggt caacacccgc ctgcccatcc ccgacgaggt gcccatgacc
     1141 cagtgggccc agatggaggc cctgggcatg aacaaggacg actacagggg ccgggtgctg
     1201 gacgtcaggc gcgacatcca gatcaagaaa cgagagatgg ccccggtgct gggctacaac
     1261 tacgacaccc tcaccgacga gcagctctcc gacatcgtgc agtacaacgt ctttcccaac
     1321 gccatcctgg ttatccagcc cgaggagctg tggatcctgc gctcgcgccc gcggggcagc
     1381 gatcccgaca agtgctactg ggacaagctg gccctgcgca tgtttccgga ccgccaggcg
     1441 cagaagcggg ccaacatctc gttcaacctg gaccaggaag gggtgcccac gggcaatgcg
     1501 cgccccgagc acgacgagtt cgagcaggag gacatcatcg ccgggcgcaa gaccatgacc
     1561 atcaccctgg atcaggacat ccacctgatc cgcgacgtcc agaaaggcat gcactcccgc
     1621 ggcttcggcg aagcttggct gaacgacgac gagagccgcg tgcagcactt ccacgcgtgg
     1681 ctcgaccgct acatggcggg gggttgaggc cagaacgagg caaaaaaaaa gcgggccatt
     1741 cggcccgctt ttcagtcgca aacctgtcgc tacatgcgat aggtcaggta caggctcacc
     1801 agacgcggcg gcgccacctg gccgtagagg tcgccgcgct cgccgggaat gatcttcgcc
     1861 agcgggcggt tggaaatggc gtaaacgtac agatcgttga agcagttcgt gcagatcacg
     1921 cccgcttccc aattcccgct atcctgactc aacgtggctc cgatgttggt caccgtacgg
     1981 ctcgggatct ccgtgaaggg ttggttcagc gtacgcttgc ccttgttgtg gtgaatggtg
     2041 tcccagtcga tggtcaggcg ccaatcacgc atcagcggca tgtcgtacgt gaagccgacg
     2101 ttggcctgga acggcgggcc accgtaacgg acgccggaga ggtcctgaat cgccacgccg
     2161 tccggaccga tgtggcatcc cgggccttgg gtgggcaggt catcgccaag cgcaccgtcc
     2221 acgggatgac atcccgcatc ttcccactcg tcgaactcca ggtggttgaa ctgtccggcc
     2281 aggcggaact gcagccggtc gttagcctgg tagatcatgt tgccctcgaa gccccagttg
     2341 tgggcc
//
LOCUS       DBNHKV010000460         5559 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1057575, whole genome shotgun sequence.
ACCESSION   DBNHKV010000460 DBNHKV010000000
VERSION     DBNHKV010000460.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1057575"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1963)
                     /locus_tag="AC16PP_15100"
     CDS             complement(2..1963)
                     /locus_tag="AC16PP_15100"
                     /inference="COORDINATES: protein motif:HMM:NF013199.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR6483007.1"
                     /translation="MRRTLCVALCFAAALLSGCSEQGPESASQAATPAAATEEAEQLQ
                     PGERGAQDYADEIFARESMPGKAVYAQHCAACHDGAVKKAPHKLMLGLMTPEAVLETL
                     STGLMKEQGALLDDEDKRLVSEYLAGVPMGTQAATAVEVCSGAAAEFDFNDPPRTTNW
                     GLQRTNTRSISAADAGLDKASLADLRLKWAFAFPGANRARSQPTLAAGAIYVGSHNGL
                     VYALDRSSGCVRWTYESAAEVRTGIVVDSWNAGDSEAKPRAYFGDVLGNVYGIDAVNG
                     TEIWRMRADEHPNATITGTPSLHDGRLFVPVSSLEVSLAADPAYECCTGRGSVVAVDA
                     ASGEMLWQTYTIEEEPTVQGQNRVETDMYGPSGATVWNSPSIDAKRNQLYVGTGENMS
                     SPATLTSDAIFAMDLETGKVKWVFQATPNDAWNSACDTDRPDSCPVEDGPDFDFGAAT
                     MLLATEKAGDLVIGGQKSGLVHALDPETGKLVWQSRVGRGGIQGGIHFGMASDGKRLY
                     VPISDMRDGRTYPDPERPGLHALDPSTGQELWSFENPDLCTGRDFCHPGISQAITSAG
                     ALVLAGGMDGVMRVHDAATGALLWERDTTETIATLSGARAFGGSFGGGSAPVVRDGLV
                     VLSSGYGIYGHMPGNLLMVLEAEEAPAAD"
     gene            2067..2942
                     /locus_tag="AC16PP_15105"
     CDS             2067..2942
                     /locus_tag="AC16PP_15105"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6483008.1"
                     /translation="MDSVRDKVAFITGAASGIGFGLASVFSREGMRVVMADVEEKALE
                     LAAGALAEGGAEVVPVQLDVTDRDAMARARDRALDAYGAVHLLCNNAGVNAAGPLYQV
                     RYEDWDWVLGVNLGGVVNGVTAFLPELMRHGRDAHVVNTASVGGLVGMPGLGIYNAAK
                     FGVVGLSEALHADLMESGVGVSVLCPGVVRTALTTSDRNRPAHLTSGQATATGESDEP
                     SANEASAMGTEPLDLAEQVLEGVLENRFFLCTHPEFREIVAERNAVLNASFTGTADPA
                     LVEAMRGLVRPIGRG"
     gene            complement(2939..3445)
                     /locus_tag="AC16PP_15110"
     CDS             complement(2939..3445)
                     /locus_tag="AC16PP_15110"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6483009.1"
                     /translation="MEEQLQELLDKKACEEVLLRYGRTQDWLDTPGQASCFWPDAEVD
                     FGFFTGNGEDWVKTVMPHEQEALRRWHMSTSIMIQVDGDRATAECYGIAAGTAEIDGE
                     LMDTVYGGRYMDELKKRGGEWRISKRTYVLDWSHRFPNALEANTGEDFALHILKIDAP
                     GHPLYREL"
     gene            complement(3482..3934)
                     /locus_tag="AC16PP_15115"
     CDS             complement(3482..3934)
                     /locus_tag="AC16PP_15115"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6483010.1"
                     /translation="MTTIAELLELEEIKNLRHMYSHYFDGKQVDKLADLFTDDAICEF
                     GPDYGGDWVGKEQIRANYATYTEAEGDEHEVMHAVTNPWIRFIDETTANGRWYLLDLR
                     TAEGAENPLILFGIYDDVYKKVDGQWLIHRTRIDFLWPKRHFHGYRDL"
     gene            4084..4911
                     /locus_tag="AC16PP_15120"
     CDS             4084..4911
                     /locus_tag="AC16PP_15120"
                     /inference="COORDINATES: protein motif:HMM:NF018038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR6483011.1"
                     /translation="MEPVRVLLVTKGHAFDHDAFLAMFEGMDELDVTMVEQPAAQVIL
                     RPENVGDYGAVLFYDMSGIPGIGLMHDGAGDTGIPPDDYRRSIEALIDGGTGIVMLNH
                     GTVSWPLWPLWRSISGSSFMLTEGELNGQRVPGSGYRGGHGPYPNPTISLTPVDPSHP
                     VAAGLGNGFTVTDEIYLKTATLGPKVTPLFESDYEFVAANFTPPPLAPPEEQAAWSHP
                     EGSNVVVWANAAGNSPVVAADIGDSPEAFGNADFRRLLRNALQWVASPEARQWVANR"
     gene            complement(4970..>5559)
                     /locus_tag="AC16PP_15125"
     CDS             complement(4970..>5559)
                     /locus_tag="AC16PP_15125"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR6483012.1"
                     /translation="KPQPGQTVVVNGAAGAVGSIAGQIARLAGARTIGITSSDEKCRW
                     LTEELKYDHAINYKAGNLYDQLAEAAPERIDVIFDNVGGDILDESLRLIGMRGVVLLC
                     GSTSQYAAEHMTGPDNYIWLGTMRARMQGFVVFDYADRYEEARRRMARWIKDGQLKLP
                     EYVVDGDIGDYPEAFLQLYQGANRGKMLLRLPAAR"
BASE COUNT          892 a         1769 c         1829 g         1069 t
ORIGIN      
        1 gtcagtcggc tgccggcgct tcctctgctt ccagcaccat caacagattg cccggcatgt
       61 gcccgtagat tccgtagccg gagctcagga ccaccagccc atccctgacc acaggggccg
      121 agccgccgcc gaacgatcca ccgaaggcgc gggcgcccga cagggtcgcg atggtttccg
      181 tggtatcgcg ctcccagagc agagcccccg tggcggcgtc gtgaactctc atgacaccgt
      241 ccatgccgcc ggccagcacc agggcgccgg ctgacgtgat ggcctgagat atgccgggat
      301 ggcagaaatc gcgcccggtg cacaggtcgg ggttttcgaa ggaccacaat tcttggcccg
      361 tgctcggatc cagcgcgtgc aggccgggcc tttccggatc cggatacgtt ctgccgtctc
      421 tcatgtcgct gatgggcacg tagagccgct tgccatccga cgccatgccg aaatgaatcc
      481 cgccctgaat gccgccgcga cccacacggc tctgccagac gagtttgccc gtctccgggt
      541 ccaacgcgtg gaccagaccc gacttctggc cgccgatgac gagatcgccg gctttttccg
      601 tggccagcag catcgtcgcg gcgccgaagt cgaagtccgg gccgtcttcc acggggcagc
      661 tgtcgggccg gtcggtgtcg catgccgagt tccaggcgtc gttcggcgtc gcctggaata
      721 cccacttcac ctttccggtt tcgaggtcca tggcgaagat cgcgtcgctg gtgagggtgg
      781 caggcgagga catgttctct ccggtgccga cgtagagctg attgcgcttt gcgtcgatgg
      841 acgggctgtt ccagaccgtg gccccggacg gtccgtacat gtccgtctcg acccggttct
      901 ggccctggac cgtgggctct tcctcgatgg tgtaggtctg ccagagcatc tcgccgctgg
      961 cggcatccac cgccaccacc gagcccctgc cggtgcagca ctcgtacgcc ggatcggccg
     1021 ccaggctgac ctccagggac gaaaccggaa cgaagagccg tccgtcatgc agcgacggtg
     1081 tgccggtgat ggtggcgttg gggtgctcgt cggcccgcat gcgccagatc tccgtgccgt
     1141 tgacggcgtc gatgccgtag acgttgccca gcacgtcgcc gaagtaggct ctgggcttcg
     1201 cctcggaatc gcctgcgttc cagctgtcga ccacgatgcc ggtgcgtacc tcggcggcgg
     1261 actcgtacgt ccagcgaacg cagccgctcg agcgatcgag cgcgtagacc agaccgttgt
     1321 ggctgcccac atagatcgcg ccagcggcca gggtgggctg ggatcgggcc cggttggcgc
     1381 ctggaaacgc gaaggcccac ttgagcctga ggtcggcaag actggctttg tcgaggccgg
     1441 catccgcggc gctgatggaa cgcgtgttgg tgcgctgcag cccccagttg gtcgtcctcg
     1501 ggggatcgtt gaagtcgaat tccgcggccg cgccggagca gacttccacc gcggtcgccg
     1561 cctgggttcc catcggcacg ccggccagat attccgatac caggcgcttg tcctcatcgt
     1621 ccagaagggc gccttgttcc ttcatcaacc cggtgctgag cgtctccaga accgcctcgg
     1681 gcgtcatcag cccgagcatg agcttgtgag gcgccttctt gactgctccg tcgtgacagg
     1741 cagcgcagtg ctgggcgtaa acggcttttc cgggcatgga ttcgcgggcg aagatctcgt
     1801 cggcataatc ctgcgcgccc cgctcgccgg gctggagctg ctctgcctcc tccgtggcgg
     1861 cggccggcgt tgccgcctga gatgctgatt cgggcccctg ttccgaacac cccgacagca
     1921 aggcggccgc gaagcagagc gcgacgcaaa gcgtacgtct cacgtaagtt ctctccccat
     1981 ggattcccaa tcgaatattg gtatcacagt ggcatggaca atgatagttt ccagccagga
     2041 atgagcgtgg aagagggaga caagcgatgg actccgttcg agacaaagtg gctttcatta
     2101 cgggtgcggc aagcggcatc ggcttcgggc tcgccagcgt gttttcgcgg gaggggatgc
     2161 gtgtggtgat ggccgacgtg gaggagaagg ccctcgagct tgctgcgggc gcgctggcgg
     2221 aaggcggggc ggaggtcgtt cccgttcagc tggacgtgac cgaccgcgac gccatggccc
     2281 gggcgcgaga tcgtgcttta gacgcctacg gcgccgtgca cctgctctgc aacaacgccg
     2341 gggtgaacgc ggcgggcccg ctctaccagg tgaggtacga agactgggat tgggtgctcg
     2401 gcgtcaatct cggtggcgtg gtgaatggcg tgactgcctt tcttccggaa ctcatgcggc
     2461 acgggcggga cgcccacgtc gtcaacaccg cttccgtggg cggcctggtg ggaatgccgg
     2521 gcctggggat ctacaacgcg gccaagttcg gcgtcgtcgg tctgtcagaa gcgctccacg
     2581 cggacctcat ggaaagcggc gtcggggtct cggtgctttg cccgggcgtg gtccgcaccg
     2641 cgctgacgac gagcgaccgc aatcgcccgg ctcacctcac cagcggccag gcgaccgcga
     2701 ccggggagag cgacgagccg agcgccaacg aggcttccgc catgggaacc gaacccctgg
     2761 acctggcaga gcaggtgctc gagggggttc tggagaaccg ctttttcctc tgcacccatc
     2821 ccgagttccg tgagatcgtc gcggagcgaa acgccgtact gaatgcgtcg tttaccggaa
     2881 ccgccgaccc cgcgttggtg gaagccatgc gaggtctggt tcgtccgatc ggtcgaggct
     2941 aaagctcccg gtacaggggg tggccgggcg cgtcgatctt cagaatgtgt agcgcgaaat
     3001 cctcgccggt gttggcctcc agcgcgttgg ggaatcgatg gctccagtcc aggacgtacg
     3061 tccgttttga gatgcgccat tcgcccccgc gcttcttcag ctcgtccatg taccggccgc
     3121 cgtagacggt gtccatgagc tcgccgtcga tctccgcggt gccggcagcg atcccgtagc
     3181 attctgcggt ggcgcgatcg ccgtcgacct ggatcatgat gctggtgctc atgtgccatc
     3241 tccgcagggc ttcctgctcg tgcggcatca cggtcttcac ccagtcctcg ccgttgccgg
     3301 tgaagaagcc gaagtccacc tccgcgtcgg gccagaagca gcttgcctgt cccggcgtat
     3361 ccagccagtc ctgggtgcgc ccgtagcgca gcagcacctc ctcgcacgcc ttcttgtcca
     3421 gcagttcctg aagctgttct tccatgctcg cgtccatcgt ttcgtccttc tgtgtttcag
     3481 atcagagatc ccggtaaccg tgaaagtggc gcttcggcca gagaaagtcg atgcgcgttc
     3541 ggtggatgag ccactgcccg tccaccttct tgtaaacatc gtcgtagatg ccgaacagaa
     3601 tgagcgggtt ctcggcaccc tcggcggtgc gcagatcaag caggtaccag cggccgttgg
     3661 ccgtcgtctc gtcgatgaaa cgaatccacg ggttggtgac cgcgtgcatg acctcgtgct
     3721 cgtccccctc tgcctccgtg tacgttgcgt agttggctcg gatctgctct ttgcctaccc
     3781 agtctccacc gtagtcgggt ccaaattcgc agatcgcatc atccgtgaag agatcggcca
     3841 gcttgtccac ctgtttgccg tcgaagtagt ggctgtacat atggcgcagg ttcttgattt
     3901 cctcgagctc gagcagctcc gcgatcgtcg tcatggttcc ctcccagggc ctgtctacct
     3961 tgacgtagat tggtgcgtat cgcaggcggg cgtcaagccg ttgcgtcgat cccgcgccgg
     4021 ggttagtttc tggcagccgc gtccgcagcg gggtttcgac taacgggaag agaggacgaa
     4081 tgaatggagc cggtgagggt acttctggtc acgaaaggcc acgcgtttga tcacgatgct
     4141 tttctggcga tgttcgaggg gatggacgaa ctcgacgtga cgatggtcga gcagccggcg
     4201 gcgcaggtca tcctgcgccc ggagaacgtc ggggactacg gcgccgtgct cttctacgac
     4261 atgtcgggta ttcccggcat cggtctcatg cacgacgggg caggggacac gggcattccg
     4321 ccggacgact accgacgcag catcgaggca ctgatcgatg gcggaacggg catcgtgatg
     4381 ctcaatcacg ggaccgtctc atggccgctg tggcccttgt ggcgatccat atcggggtct
     4441 tctttcatgc tgaccgaagg tgagctgaac ggccagcgcg tgccggggtc cgggtaccgg
     4501 ggcggtcatg gtccgtatcc caacccgacc atcagcctga cgcccgtcga tccttctcat
     4561 cccgtggctg cgggcctggg aaacggcttc accgtgacgg acgagattta cctcaagacg
     4621 gctacgcttg gtccgaaggt gacgccgctg ttcgaaagcg actacgagtt cgtcgcggct
     4681 aacttcacgc cgccgcccct ggccccgcca gaggagcagg ctgcgtggtc gcacccggaa
     4741 gggagtaacg tcgtggtctg ggcaaacgcg gccggaaaca gcccggtggt ggcggccgac
     4801 atcggtgaca gcccggaggc tttcggcaac gccgattttc gtcgtctgct gcgcaacgcc
     4861 ctgcagtggg tggcgtcgcc ggaagcccgg cagtgggtgg cgaaccggta ggagatcaaa
     4921 accggatcgt cgcccggggc atgttcgggc ccggagttgg gggcgaaagt cagcgggcgg
     4981 ccggcagccg cagaagcatt ttgccccggt tcgcgccctg atagagctgc agaaaggctt
     5041 ccggatagtc gccgatatcg ccgtcgacga cgtactccgg cagcttcagc tggccatctt
     5101 tgatccagcg cgccatcctg cggcgggctt cctcgtagcg gtccgcgtag tcgaaaacca
     5161 cgaatccctg cattcgcgcc cgcatggtac ccagccatat gtaattgtcc gggccggtca
     5221 tgtgctcggc ggcgtactga gaggtggagc cacacaggag caccacgccc cgcatgccga
     5281 tcagccgcag gctctcgtcg aggatatcgc cgcccacgtt gtcgaagatc acgtcgatcc
     5341 gttcgggtgc agcctcggcc agctggtcgt agaggttacc ggccttgtag ttgatggcgt
     5401 gatcgtattt cagttcttcg gttagccagc ggcacttctc gtcgctgctc gtgataccga
     5461 tggtccgcgc gccggctagc cgcgcgatct gcccggcgat ggagccaacg gcgccggccg
     5521 ccccgttcac gaccaccgtc tggcctggct ggggtttgc
//
LOCUS       DBNHKV010000461         5657 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1694849, whole genome shotgun sequence.
ACCESSION   DBNHKV010000461 DBNHKV010000000
VERSION     DBNHKV010000461.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1694849"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="AC16PP_15130"
     CDS             <1..513
                     /locus_tag="AC16PP_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HIR6483013.1"
                     /translation="LVLLRELGPVVTGLLFAGRAGSAITAEIGLMKTTEQLSSMEMMG
                     VDPLRRIVAPRLWAGVISMPLLTAIFNVVAIYGGLVVAVEWLGGDPGGFWSGMQDAVE
                     LWHDVGKGMLKSVVFAVVVTWIAVFQGYDTEPTAEGIGLATTRTVVASSVMVLAIDFL
                     LTLSMYGEFN"
     gene            513..971
                     /gene="mlaD"
                     /locus_tag="AC16PP_15135"
     CDS             513..971
                     /gene="mlaD"
                     /locus_tag="AC16PP_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HIR6483014.1"
                     /translation="MHIRTLEISVGAFMLAGILALVFLAVKVSGISLDADRGSYQLVA
                     RFDDVAGLRERAKVSIAGVQIGRVALIEVDTIYGQAVVTLEIDGSPGGLSADTGAQIL
                     TEGILGARYVNLLPGADDETLADGDLIYDTQGALVLENLVGDLITRLGAG"
     gene            971..1735
                     /locus_tag="AC16PP_15140"
     CDS             971..1735
                     /locus_tag="AC16PP_15140"
                     /inference="COORDINATES: protein motif:HMM:NF017319.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaC/ttg2D family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR6483015.1"
                     /translation="MTMRLMNHSGLESGFVLAIAALLLAGLAPARAMEPSDVATRTAT
                     EVVERTSDEMLALIDASRPYVEDDPERFYGEVESLLAPVVDFKRFARSVMAVHYKSAT
                     PEQRDRFADTFKWGLVRTYALALTEFRDGEVVIVPSERPPQSPRRESVKMEIRMNTGD
                     VYPVVYSMAQGKDGAWRMGNIIVNGVNIGLTYRSQFASAVQDQRYGGDLDKVIDAWTL
                     LLANETPGAASADSGGSDEADEAPSSDGAGGAGDDR"
     gene            1732..2022
                     /locus_tag="AC16PP_15145"
     CDS             1732..2022
                     /locus_tag="AC16PP_15145"
                     /inference="COORDINATES: protein motif:HMM:NF024857.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR6483016.1"
                     /translation="MSAFSLPPRVGVDNFAAVRAAGEQFLAERRDEKAVVDLSGLESC
                     NSIAVALLMTWFRFAHARGQEVVYAGVPQDLLSIIEISGLMEVLPLSRDAAG"
     gene            2052..2303
                     /locus_tag="AC16PP_15150"
     CDS             2052..2303
                     /locus_tag="AC16PP_15150"
                     /inference="COORDINATES: protein motif:HMM:NF013851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HIR6483017.1"
                     /translation="MQEEIAASITERLSGEDDALRVDVVVEGNRAMITVVSRMFEGMT
                     RVRKQQTVYGCIDAFIQDGRLHAVSIRSLTPEEAAGSSG"
     gene            2296..3561
                     /gene="murA"
                     /locus_tag="AC16PP_15155"
     CDS             2296..3561
                     /gene="murA"
                     /locus_tag="AC16PP_15155"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR6483018.1"
                     /translation="MDKLRITGGRTLSGSLRVSGAKNAALPILAGTLLADEPVTLSNV
                     PHLQDVTTMIELLGCMGARVLVDEKMRVEVSADSLAQLRAPYELVKTMRASFVVLGPL
                     LARFGEAEVSLPGGCAIGARPVDQHVKGLEALGAEISVNEGYVRGKVGGRLRGADFAF
                     DMVTVGATENLMMAAALAEGVTRLRNAACEPEVADLGDFLNAMGAKVSGHGSPEIRIE
                     GVPRLHGCEYRVMPDRVEAGTYLIAVAATGGRIRLTDVRPDTLTAVLDKLVESGAEVE
                     VGDDWIELDMHGRRPRAVDITTAPYPGFPTDMQAQYLAMNAIAEGTSEVSETIFENRF
                     MHVQELSRLGADIELHGPSSVSVRGVPSLKAAPVMATDLRASFSLVVAALVAEGTTLI
                     DRIYHIDRGYETIEEKLKLLGAEVQRVSG"
     gene            3577..4203
                     /gene="hisG"
                     /locus_tag="AC16PP_15160"
     CDS             3577..4203
                     /gene="hisG"
                     /locus_tag="AC16PP_15160"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR6483019.1"
                     /translation="MGLTIALNRGRILEESLPMLAAAGIVPEEDPAASRKLIFATRQG
                     GHRLVVMRGADVPTYVEYGAADVGIVGKDTLLEHGGSADYYERLDLGIAKCRIMVAAP
                     AGADLGSGKLRVATKFPRVARRYFEGQGRQVSIIKLNGAMEIAPLMNLADCIVDIVDT
                     GNTLRANGLEALETIAHVSSRMIVNRAAMKTRFDAVQSLTCSLRDVSG"
     gene            4200..5528
                     /gene="hisD"
                     /locus_tag="AC16PP_15165"
     CDS             4200..5528
                     /gene="hisD"
                     /locus_tag="AC16PP_15165"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR6483020.1"
                     /translation="MTEPALEIARLATGDADFPDRLARLLYWDTGLEDEVTRAAAEVI
                     EAVRAGGDDALLALTARYDRYECPSAAALEIAASELEAAYDALGSADREALNLAAQRI
                     REYHNHQLAGDWEYTDSDGNLLGQRVMALERVGLYVPGGQAAYPSTVLMTAVPARVAG
                     VDEIIMTVPTPDGVRSPLVLAAAHVAGVDRVFTVGGAQAIAALAYGTPTIPKVDKIVG
                     PGGAYVAAAKRLVFGTVGIDVIAGPSEVLVIADDTAPPEWLALDLFSQAEHDASAQAI
                     LVSPDPDVLDAVHDAMASLIGERSRATTIRASLAARGALIRAADLADAVALANRIAPE
                     HLELAVADPDALLPDVRNAGAIFIGAHSPEVMGDYVAGPSHVLPTFGTARFSSPLGVY
                     DFVKRSSVIRLSPGGSEKLARASATLARGEGLEAHARAAELRITGLDHNR"
BASE COUNT          942 a         1780 c         1957 g          978 t
ORIGIN      
        1 ctggtgctgc tgcgcgagct cgggccggtg gtgaccgggc tgctgttcgc cggtcgggca
       61 ggttccgcca tcacggcgga gatcgggctc atgaaaacca ccgagcagct gtcgagcatg
      121 gagatgatgg gcgtcgatcc gctgcgccgg atcgtggcgc cgcggctttg ggccggggtc
      181 atcagcatgc ctctgctcac cgccatcttc aacgtcgtgg ccatctacgg tggcctcgtg
      241 gtcgccgtgg agtggctggg cggcgatccc ggcggcttct ggtccggtat gcaggacgcg
      301 gtggagctat ggcacgacgt cggcaaaggc atgctgaaga gcgtcgtgtt tgcggtggtc
      361 gtcacttgga tcgccgtttt tcaggggtac gataccgaac cgacggctga gggaatcggc
      421 ctggcgacta ccaggacggt cgtggcctcc tcggtgatgg tgctcgcaat cgacttcttg
      481 ctcacgctga gcatgtacgg agagttcaac tgatgcacat acgaacccta gagatcagcg
      541 ttggcgcgtt catgcttgct ggaatccttg cgctcgtctt tctcgccgtg aaggtcagcg
      601 gcatcagtct ggatgcagat cgtggcagct atcagttggt ggcgcggttc gacgacgtgg
      661 ccggcctgcg cgagcgtgcc aaggtgagca ttgccggtgt ccagatcggg cgcgtggcgc
      721 tcatcgaggt cgacaccatc tacggccagg ccgtggtgac cctcgaaatc gacggcagtc
      781 cgggcggcct ttccgccgac acgggggcgc agatactcac cgaaggaatt ctcggtgcgc
      841 gctacgtgaa tctgctgccg ggggcagatg acgagacgct ggcggacggc gatctgattt
      901 acgataccca gggcgccctc gtgctggaga acctggtcgg cgacctcata acgcggcttg
      961 gagcaggctg atgacgatgc gtttgatgaa tcactcgggt cttgaatccg gcttcgtgct
     1021 cgcgatcgcc gccctgctgc tcgcaggcct cgcgcccgct cgcgccatgg agccctcgga
     1081 cgtcgcgacc cgtaccgcca ccgaggtggt ggagcgcacg tcggacgaga tgctcgcgct
     1141 catcgatgca tccagaccgt acgtcgagga tgacccggaa cggttctacg gcgaggtgga
     1201 gtcgctgctc gcgccggtgg tcgacttcaa gcggtttgcc cgcagcgtca tggccgtgca
     1261 ctacaaatcc gccactcccg agcagcgcga tcgcttcgcc gataccttca aatggggcct
     1321 ggttcgaacc tatgcgctgg cgctgacgga gttcagggac ggcgaggtgg tcatcgtgcc
     1381 ctccgagcgg ccgccccaaa gcccccgccg cgagagcgtg aagatggaaa tccgcatgaa
     1441 taccggagat gtatacccgg tggtttactc aatggcgcag ggcaaggacg gcgcctggcg
     1501 catggggaac atcatcgtca acggcgtgaa catcggtctg acctaccgca gccagtttgc
     1561 cagcgccgtg caggatcaga gatacggggg ggacctggac aaggtcatcg acgcctggac
     1621 tttgctcctg gccaacgaga caccgggtgc cgcgtcggct gactcgggcg ggtcagacga
     1681 ggcggacgag gcgccgtcga gcgacggggc cggcggagct ggtgacgatc gttgagcgcg
     1741 ttttccctgc cgcctcgcgt cggcgtcgac aacttcgctg cggtcagggc ggctggcgag
     1801 cagtttctcg ccgaacggcg cgacgagaaa gccgtcgtcg atctttccgg cctggagagc
     1861 tgtaacagca tagccgtcgc ccttctgatg acctggtttc gctttgccca tgcgcggggg
     1921 caggaggtgg tttatgctgg cgtcccccag gatttgctca gcatcattga aatttccggg
     1981 ctgatggaag ttctgccgct ttcccgggac gcggcaggct gaacctttaa ccgactgcgg
     2041 cagggcggga catgcaggaa gaaatcgcgg cgagcatcac agaaaggctg tccggggagg
     2101 acgacgcctt gcgcgtggac gtcgtcgtcg aggggaaccg cgcgatgatc accgtggtca
     2161 gccggatgtt cgagggcatg acgcgcgttc gcaagcaaca gaccgtttac ggatgcatcg
     2221 acgctttcat ccaggacggt cgcctgcacg ccgtgagcat tcgctccctc acgcccgagg
     2281 aggcggcggg aagcagtgga taagctccgc ataacgggcg ggcgcacgct gtccggctcg
     2341 ctgcgggtct ctggcgcaaa aaacgcggcg ctgccgatcc tggcgggcac gcttctggcc
     2401 gacgagccgg tgacgttgag caacgtccct catctccagg acgtcaccac catgatcgag
     2461 ctgctcggct gcatgggcgc ccgcgtgctc gtcgacgaga agatgcgcgt cgaggtttcg
     2521 gccgacagcc tggcgcaact gcgggcgccc tacgagctgg tgaaaaccat gcgcgcctcg
     2581 ttcgtcgtgc tcgggccgct gttggcgcgc ttcggcgaag cggaggtctc ccttccgggc
     2641 ggttgcgcca tcggggcccg cccagtggac cagcacgtca aaggcctgga agccctcggt
     2701 gcggagatct ccgtgaacga gggttacgtt cggggcaagg tcggcggccg gctccgcggg
     2761 gccgacttcg cgttcgacat ggtgacggtg ggcgcaaccg agaacctcat gatggcggcc
     2821 gcgctggccg agggggtcac gcggctgcgc aacgccgcct gcgaaccgga agtggccgac
     2881 ctcggagatt ttctcaacgc catgggcgcg aaggtttcgg gtcacggctc cccggaaata
     2941 agaatcgaag gcgtgccgcg gttgcacgga tgcgagtacc gggttatgcc cgatcgcgtc
     3001 gaagccggga cttacctgat tgccgttgcg gccaccgggg gcaggataag gctcaccgac
     3061 gttcgacccg acacccttac ggccgtgctc gacaagcttg tggaaagcgg cgccgaggtc
     3121 gaagttggcg acgactggat cgagctcgac atgcacggcc ggcgaccgcg cgcggtggat
     3181 atcaccacgg cgccatatcc cggctttccg acggacatgc aggcccagta cctggcgatg
     3241 aacgccatcg ccgagggaac cagcgaggtc tcggaaacca tcttcgagaa ccgcttcatg
     3301 cacgttcagg agctctcgcg actcggcgcg gacatcgagc tgcacggccc gtccagcgtc
     3361 tcggtgcgcg gcgtgccgtc gctgaaagcc gctcccgtca tggccacgga cttaagagcg
     3421 tcgttcagcc tggtggtcgc cgcgctcgtt gcggaaggca ccacgctcat cgataggatc
     3481 tatcacatcg accgcggcta cgagaccatc gaggagaagc tcaaactgct cggcgctgag
     3541 gtccagcggg tttcgggctg atcgagagga attgaaatgg gtctgaccat agcgctcaac
     3601 cggggcagga tcctcgagga gtccctgcca atgctggctg cggcgggcat cgtcccggag
     3661 gaggacccgg ccgccagccg caagctcatt tttgccaccc ggcagggcgg gcatcgcctg
     3721 gtggtgatgc gtggtgccga tgtccccacc tacgtggaat acggggcggc ggacgtgggc
     3781 atcgtgggca aggacaccct gctggagcac ggcggcagcg cggattacta cgagcgcctg
     3841 gacctgggca tcgcgaagtg ccgcatcatg gtggctgctc ccgcaggcgc ggatctggga
     3901 tccggcaagc tgcgggtggc gacgaagttt ccgcgggtgg cccgtcgcta cttcgaaggt
     3961 caggggcgtc aggtatccat catcaagcta aacggcgcca tggagatcgc cccgctcatg
     4021 aacctggcgg attgcatcgt ggacatcgtg gacaccggaa acaccttgcg cgcgaacggc
     4081 ctggaagctc ttgagaccat tgcgcacgtc tcctcgcgaa tgatcgtaaa ccgggcggcc
     4141 atgaagaccc gcttcgacgc cgtgcagagc ctcacctgca gcctgcggga cgtgtcggga
     4201 tgacggaacc ggcgctcgag attgcccggc tcgcgacggg cgatgcggac ttcccggatc
     4261 gtctcgccag gctcctttac tgggacacgg gcctggaaga cgaggtgacg cgagcggcgg
     4321 ccgaggtcat agaagcggtg cgggccggtg gtgacgacgc cctgctggcg ctcaccgcca
     4381 ggtacgatcg ttacgagtgc ccgagcgccg cggcgctgga gatcgcggct tccgagctcg
     4441 aggcagccta cgacgccctg ggctcagcag accgcgaggc gttgaacctg gccgcccagc
     4501 ggattcgcga atatcacaac caccagctgg ccggagactg ggaatatacc gacagtgacg
     4561 gcaacctcct cggccagcgg gtgatggcgc tggagcgggt gggcctctac gtgcctggcg
     4621 gccaggcggc gtacccgtca acggtgctga tgacggcggt gcccgcccgg gtcgcgggtg
     4681 tggacgagat tatcatgacc gtccccacgc cggatggcgt gcgttcgccg ctggtgctcg
     4741 ccgcagccca tgtagcgggc gtcgaccggg tatttaccgt tggcggcgcc caggccatcg
     4801 ccgcgctggc ctacggcacg ccgacgattc ccaaggtgga caagatcgtc ggtcccggcg
     4861 gggcatacgt cgctgccgcc aagcgcctgg ttttcggcac cgtcggtatc gacgtcattg
     4921 ccggccccag tgaggtgctt gtgattgcgg acgacacggc gccgcccgag tggctcgccc
     4981 tggacctgtt ttcccaggcg gagcacgatg cttccgccca ggccattctg gtcagcccgg
     5041 acccggacgt gctcgatgcc gtccacgacg ccatggcctc gctcatcggc gagcggtcca
     5101 gagcaacgac catccgagcc tcgctcgccg cgcgaggtgc cttgatccgg gcggcggacc
     5161 tcgccgacgc cgtggcgctg gccaaccgaa tcgcgcccga gcatctcgag ctcgccgtgg
     5221 cggatccgga cgcgctcctg cccgacgttc gcaacgccgg cgcgatcttc atcggcgcgc
     5281 actctccgga ggtgatgggc gattacgtgg cgggtccgag ccacgtgctg ccgacattcg
     5341 gtacggccag gttctcgtca ccgctggggg tctacgattt cgtcaaacgc agctccgtga
     5401 tccggctgag tccaggcggg tccgaaaagc ttgcccgcgc ctcggcgacc ctcgctcggg
     5461 gcgaggggct cgaagcccac gccagggccg ctgaattgag gatcaccggg ctggaccaca
     5521 accgttagcc ggacccgtga ggctcctagg gctgagggcg ctccccggcg cgcgccgtga
     5581 gggagatctg gcgattccca cgcagaacct ctatctccag ttcgctgccc ggctcgacgt
     5641 gagcgatctg ccgcgtg
//
LOCUS       DBNHKV010000462         5516 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_987807, whole genome shotgun sequence.
ACCESSION   DBNHKV010000462 DBNHKV010000000
VERSION     DBNHKV010000462.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_987807"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="AC16PP_15170"
     CDS             <1..486
                     /locus_tag="AC16PP_15170"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6483021.1"
                     /translation="LEFRRVLFRSLAGLEYDVFTASSVMSESFGRYPFNNANERALIT
                     LAVEEDFEIHAPRLLVVHVLFNLIKNALYYVQRAGKGSITISCARRCITVHDTGSGIS
                     RVARSQVFDRFYTTTSTGQGAGIGLSFCKMVMESIGGSITFESAEDEHTTFILEFPAA
                     T"
     gene            662..1930
                     /locus_tag="AC16PP_15175"
     CDS             662..1930
                     /locus_tag="AC16PP_15175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR6483022.1"
                     /translation="MMLGVSSSILVAMLEIGFIGQLGTESVAAITFTFPVVMILSSIA
                     LGVGIGTSSVIARSVGAGQLEDGRRLGTHSLLLVVAMISALSFIGWLAIDPVFLALGA
                     PAHMLPLIHSYLDIYYPSVVFFATTMAAGNIMRASGSANVPGLVMTLGAVVNLALDPI
                     FIFGWFGFPRLELAGAAVAGAISRVGTAAILLYFVARGGLLLTTGWKPRFLNSCHRIL
                     EVGLPAMATQLIGPVSSAVITRLLAGHGEIVVAGFGVATRVEAVAVMLLFALSGSIGP
                     FVGQNWGAGRSERVREGLRFTYLFCLAWGLAAAILMAFFGSTVASWVDSEPGVIGTAA
                     FYLAVVPWSYGLWGVLMMSSASFNALGKPIPSTILSFTRMFIMYIPLAVLLEGQFGYT
                     GIFVATAATNGVMGILAFLWFRSAFFADAR"
     gene            1935..2900
                     /locus_tag="AC16PP_15180"
     CDS             1935..2900
                     /locus_tag="AC16PP_15180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6483023.1"
                     /translation="MITIPAFIAATVAVTAALPVLAIAAAICSVVPATKGALPTLGFI
                     AGYLWCETIGIVTAFAIWIRHREADRFLQANFRLQCWWANALKVIAERLFRLTFDVEG
                     EECLSGPAAIMLPRHASIADTIIPMVYYAIPRQIRLRYVLKRELLADPCLDIVGHRLP
                     NYFVDRHGQDSERARRGVAALLADIGDDEGALIYPEGTRFSEAKRSRLLERYAASEDM
                     LAQLRRWTELLPPRLGGTLALLDANPGRDLIFCAHTGFEGSSHFATLINGSWIGAHIR
                     ISFWRVPGQAVPAETDAQREFLFDQWDRMQAEVLRLQAAPGGTAP"
     gene            2897..3091
                     /locus_tag="AC16PP_15185"
     CDS             2897..3091
                     /locus_tag="AC16PP_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017738733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HIR6483024.1"
                     /translation="MTHYEVRSVSLSCPYCGETIEILIDLSLSEQEYIEDCSVCCRPI
                     LLDVSVTDDEPLVTARSENE"
     gene            complement(3096..3869)
                     /locus_tag="AC16PP_15190"
     CDS             complement(3096..3869)
                     /locus_tag="AC16PP_15190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6483025.1"
                     /translation="MSAVVNVRSKKVPTLYEADESKYAGPHRWQIRAWARVARRLWRY
                     RAYTRWARQAFSRIEVSGAEHLDSVEGPCVFIANHQSHLDTILVHAVLPEKIRSKIYF
                     GAAQDRWFVKGKRKLVLKPWYQSLALGNFPILRGGGTRALAYASWLLDRGQHVFLFPE
                     GTRATSTEVGEFKHGAAILALEAGVPVVPVYIAGAQKIRPKGTREVARGVGGIEILEP
                     VRFSAGSDVAAATRLLQDRLGEVHARHRGGDRERLPSAA"
     gene            complement(4104..4697)
                     /locus_tag="AC16PP_15195"
     CDS             complement(4104..4697)
                     /locus_tag="AC16PP_15195"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6483026.1"
                     /translation="MELILVRHGLPVREELETGRADPPLSETGRAQAERVALWLADAP
                     VDAVYSSTMQRARQTAQPYAAAIGLEVVEREGIAEFDRHESSYVPLEELKAEDPEAWR
                     AFVAGGYNEQVDIHQFHAEVVNSLEQLIARHSGQRIAVFCHGGVINVWTAHVIGITPR
                     LFFEPAYTSIHRFLCARSGERNLVSLNETGHLRAPIT"
     gene            4917..>5516
                     /locus_tag="AC16PP_15200"
     CDS             4917..>5516
                     /locus_tag="AC16PP_15200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase family protein"
                     /protein_id="HIR6483027.1"
                     /translation="MVGVYRIHEAGGPEVLSWESEELPDPTGELVRIRHTAIGLNYID
                     TYHRSGLYPLPYPTRLGVEAAGFVEAVGDAVTDLKEGDRVAYAGGMGAYAEANNVPAS
                     RLVRIPDNLADETVAACLLKGLTACYLLTRTYPLAANDTMLLHAAAGGTGLIMCQWAR
                     HIGATVIGTAGSQEKAELAEHNGADHTILYREEDVVARVR"
BASE COUNT          946 a         1776 c         1716 g         1078 t
ORIGIN      
        1 ctggagttca gacgtgtgct cttccgatct ttggccggac tggagtacga cgtgttcacg
       61 gcgtcctcag tcatgtcaga gagtttcggg cgttacccct tcaacaacgc taacgagcgg
      121 gcgctcatta ctctcgcggt cgaggaggat tttgaaatcc acgcacctcg cctgctggtg
      181 gttcacgtcc ttttcaacct gatcaagaat gctctctact acgtgcagcg agcaggcaag
      241 ggctcgatca cgatctcttg tgctcgacgc tgcatcaccg tgcacgatac cggatcaggt
      301 atttcccgcg tcgccaggag tcaggtgttc gatcggttct acactaccac cagcaccgga
      361 cagggtgcgg gaatcgggtt gagcttctgc aagatggtca tggaatccat cgggggcagc
      421 attaccttcg agtcggcgga agacgagcac accaccttca ttctcgagtt tcccgcggcg
      481 acctagcttc agcatctgcg ccgtctcacc ggccgcaacc gctgcctgaa aaacggccct
      541 cccgctagga gccctggcac ggtgcgctta aactgcaccg tcgctcggct gatactcaat
      601 gccccacacg gatctgacac aaggcccagt tgccacatca ctgtttcgtc tgacggcgcc
      661 gatgatgctt ggcgtatcga gcagtatcct cgtcgcgatg ctcgagatcg ggttcatcgg
      721 gcagctgggt acggaatcgg tcgccgccat caccttcacc tttccggtcg tgatgatcct
      781 ttcgagcatc gcgctgggtg tcggaatcgg gacctcctcc gtaatcgccc gcagcgttgg
      841 ggccggccag cttgaggatg gccggcgcct cgggacccac agcctgctgc tggtcgtggc
      901 catgatatcg gctctgtctt tcattggttg gctggctatc gatccggtct ttctggctct
      961 tggcgcccct gcacacatgc tgccgctcat ccacagctac ctcgacatct actatccgag
     1021 cgtcgtgttt ttcgccacca ccatggccgc cggcaacatc atgcgcgcca gcggaagcgc
     1081 caacgtgccc ggcctggtga tgacccttgg cgcggtggtc aaccttgcgc tcgatccgat
     1141 tttcatcttc ggctggttcg ggtttccacg gctggaactt gcaggcgcgg ccgtggccgg
     1201 agccatctcc agagtaggca cggccgcgat tctgctctat ttcgttgcca ggggcggcct
     1261 gttgctgaca accgggtgga agcccagatt tctcaactcg tgccatcgga ttctggaggt
     1321 cggccttccc gccatggcca cccagctcat cggaccggtt tcctcggccg tgatcactcg
     1381 actcctggcc ggtcatgggg agatcgtggt tgcgggcttc ggagttgcga cccgggtcga
     1441 ggccgtggcg gtcatgttgc tcttcgcctt gtccggcagc attggtccat tcgttggcca
     1501 gaactggggc gcaggccggt ccgagcgtgt ccgggaaggc ctgcgcttca cctacctctt
     1561 ctgcctggcc tgggggctgg cagccgcgat tctcatggcg ttcttcggca gcaccgtcgc
     1621 atcatgggta gactctgagc ccggtgtgat cggaaccgcg gcgttttatc ttgcggtggt
     1681 cccctggagc tacggtctct ggggcgttct gatgatgtca tcggcgtcgt tcaacgcgct
     1741 gggcaagccg ataccctcca ccatcctctc gtttacgcgg atgttcatca tgtacattcc
     1801 gctggccgtg ctgctggaag ggcagttcgg ctatacggga atattcgtcg cgaccgcagc
     1861 aaccaacggc gtcatgggca tactggcgtt cctctggttc cgaagcgcgt tttttgcaga
     1921 tgctcgttag gcgcttgatc acgattccgg cattcattgc ggccacggtg gccgttaccg
     1981 cggcgcttcc cgtgctggcg atagcggcgg ccatttgcag cgtcgttcca gctaccaaag
     2041 gcgcgctgcc gacgcttgga ttcatcgccg gctacctgtg gtgcgagacc atcgggatcg
     2101 tcacggcatt cgcgatctgg atccggcatc gggaggcaga ccgattcctc caggcgaact
     2161 tcaggctcca atgctggtgg gccaacgcgc tcaaggtcat cgcggagagg ctcttcaggc
     2221 tgaccttcga tgtcgagggc gaagagtgtc tttcgggtcc ggcggcaatc atgctgccgc
     2281 gacacgccag catcgccgac acgatcatcc ccatggtcta ctacgcaatt ccccggcaaa
     2341 tcaggctcag gtacgtgctg aaacgggagc tgctggctga cccctgcctg gacatagtcg
     2401 gccaccggct acccaactac ttcgtcgatc ggcacggcca ggacagcgag cgggcccgac
     2461 gaggtgtggc cgcgttgctc gccgacatcg gtgatgatga gggcgcgctg atctacccgg
     2521 aaggcacccg gttctccgaa gcaaaacgat cgcggttgct cgagcgctac gcggcttcgg
     2581 aggacatgct cgcccagctt agacgctgga ccgaactgct gcccccgcgt ctgggcggaa
     2641 cccttgcgct gctcgatgcc aatccggggc gggatctgat tttctgcgcg cacacgggct
     2701 tcgaggggtc gagccacttc gccacgctca tcaatggatc ctggatcggc gcccacatcc
     2761 gcatctcctt ctggcgcgtg ccaggccagg ccgtgccggc ggagaccgat gcccagcgcg
     2821 aattcctgtt cgaccaatgg gacaggatgc aggctgaggt gcttcgtctg caggccgctc
     2881 cgggaggaac ggccccgtga cccactacga ggttcgctcc gtctccctga gctgtccgta
     2941 ttgcggcgag accatcgaga ttctgatcga cctcagcctg tcggagcagg aatacatcga
     3001 agactgctcc gtttgctgcc ggcccatcct gctggacgtc tccgtgaccg acgacgagcc
     3061 cctggtgacc gcgcgcagcg agaacgagtg acgcgctagg cggcgctagg cagcctctcc
     3121 cgatcaccac ccctgtgccg ggcatgaacc tcacccagac ggtcctgcag aagccgggtc
     3181 gcggcggcta cgtcgctccc cgcagagaac ctcaccggtt ccagaatctc gattccaccc
     3241 acccctcggg caacctcccg cgtgcctttg ggcctgattt tctgcgctcc cgcgatgtag
     3301 acgggcacga cgggaacgcc tgcttcgagc gcgaggatgg ccgcgccgtg cttgaactcc
     3361 ccgacctccg tcgaggttgc acgggtgccc tcgggaaaga ggaacacgtg ctgcccacgg
     3421 tcgagcagcc agctggcgta cgccagcgct ctggtgccgc cgccacggag gatgggaaag
     3481 tttccgagag ccagcgactg ataccagggc ttgagtacga gcttcctctt acctttgacg
     3541 aaccatctat cctgagctgc gccgaagtag attttcgaac ggatcttctc cggcagcacg
     3601 gcgtgtacca gtatggtgtc gagatggctc tggtgattgg caatgaagac gcaaggcccc
     3661 tcaaccgagt cgaggtgctc cgcacccgag acttcgatgc ggctgaacgc ctgccgtgcc
     3721 caacgggtgt atgcccggta gcgccagagc cgccgagcca cccgtgccca cgcccgaatc
     3781 tgccaccggt gcggcccggc gtacttcgac tcgtccgcct cgtacagggt ggggaccttt
     3841 ttgctcctga cgtttactac cgccgacatc actgacctcc aatgctttgt tgatgacctc
     3901 gcccgaagga gcgttgcact ccaggacgcg tccatgacag gaccgttacc ccatggagat
     3961 tgcaatttgc aggccaaagt gaaggcaccg tatttcggcc ttcgcccggc atcggcgggt
     4021 aaccggcgca ccaacttggg accactgggt tcggccgcgc accacttcga tgcaaagcga
     4081 gacgcggccg gtcggatggg aggtcaggta atcggcgcgc gcaggtgccc ggtctcgttg
     4141 aggctgacca gattgcgctc accggaacga gcgcacagga accggtggat actcgtgtaa
     4201 gccggctcga agaacagacg aggcgtgatc ccgatgacgt gcgccgtcca cacattgatc
     4261 acgccgccgt ggcagaacac ggcaattcgc tgaccggagt ggcgggcgat gagctgttcc
     4321 agggagttca ccacctcggc gtggaactgg tgaatgtcca cctgctcgtt atagccgccg
     4381 gcgacgaacg cccgccacgc ttccggatcc tcagctttga gctcctcgag gggcacgtag
     4441 ctggactcgt ggcggtcgaa ctcggcgatt ccctcccgct cgacgacctc cagcccgata
     4501 gcggccgcgt agggctgtgc cgtctgccgc gccctttgca tggtgctcga gtagacggca
     4561 tcgacgggcg catcggccag ccacagcgcg acgcgctcag cctgcgccct gccggtctct
     4621 gaaagcggcg ggtccgcacg tccggtctcc agttcctcgc gaacgggtaa tccgtgacga
     4681 acgagtatca actccatgat gaggtcctct gtggtgtctt cggcctgcga cggcggaccg
     4741 cccggaagct ggaggtgccg gatcggccgc ggggcgggcc gagcgacccg cgtcgaagta
     4801 tagccgaggc tcaccgacca gcggcacggc agggctcgtc gggtcattgc agggcgattg
     4861 cacctccagg cgccatggcc gagacttgcg gttcatgact cgattgggga gggagcatgg
     4921 tcggcgtcta tcgcatccac gaagcaggtg gtcccgaggt tctcagctgg gagtcggaag
     4981 agcttcccga cccgaccggc gagctggtgc gcatcagaca cactgcgatc ggtctgaatt
     5041 acatcgacac ctaccatcgc agcggtctct acccacttcc ctaccccacc cgactcggcg
     5101 tggaagcggc aggcttcgtc gaggccgtag gggacgccgt gacggatcta aaggaagggg
     5161 atcgagtggc atatgcgggc gggatgggcg cctatgcgga ggcaaacaac gtgcctgcca
     5221 gcagactggt ccgtatcccg gacaacctcg ccgacgagac ggtcgccgcc tgcctgctca
     5281 agggactcac agcctgctac ctgctgacgc gcacctaccc cctggccgcc aacgacacga
     5341 tgctgctcca tgccgcggcc ggcggtaccg gcctgatcat gtgccagtgg gcgcgccata
     5401 tcggagcgac ggtcatcggg acagcgggta gccaggagaa ggccgagctg gccgagcaca
     5461 acggtgccga ccacacgatt ctttatcggg aggaggacgt ggtagccagg gtgagg
//
LOCUS       DBNHKV010000463         1839 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1694959, whole genome shotgun sequence.
ACCESSION   DBNHKV010000463 DBNHKV010000000
VERSION     DBNHKV010000463.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1694959"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1202)
                     /locus_tag="AC16PP_15205"
     CDS             complement(45..1202)
                     /locus_tag="AC16PP_15205"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6483028.1"
                     /translation="MDDELDLMGPEEMADPAVSARRLLEGPRCRLYHGFDPPFYVVSR
                     YDDVSAIVRDPGRFLSGHGQGPNFVAPMGVVSDPPQHTFFRELVQPDFLPRSIALLRP
                     RLEAIADELLDAVAEETVWDVHDRLSFPLPVTIICEIFGIPTDDIEQFKIWSDASVAA
                     LSSQNPQQYDAELARMQAYVLERLRGTRRRPGDGSLISRIARARRDGVPVTDEEAVRL
                     VLQLFVAGNETTTSLITNFVWRMLAGDGLWADFCAGRVDVDRAINESLRFDPPLLGLF
                     RTTAVDVDVGGTVIPAHSKVLTHYGAANRDPAAFRDPDVFDVGRTGRKVLSFGLGIHV
                     CLGRELALLEARVALQGLARRFPHLELVDDGERIGPFLFWGRRHLPVRNRA"
     gene            complement(1195..>1839)
                     /locus_tag="AC16PP_15210"
     CDS             complement(1195..>1839)
                     /locus_tag="AC16PP_15210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HIR6483029.1"
                     /translation="DGFRQDLMELMLYLGDPVEGQRVHAVPGAGSKVPVWILGSSLFG
                     AEVAAALGLPYAFASHFAPAAMDQAIEVYRKNFKPSEHLDAPYVMLGFNVCAADTEEE
                     AVYLRSSSLQAILQLRQGAPGRLPPPVHDFDATLTPEQRYLIDRFSLCSAVGTRETVE
                     RSINDFLARTGADELIIVGQIFDHQARLRSFEIVAELIGDLQPGDSTRVAGQHG"
BASE COUNT          349 a          591 c          625 g          274 t
ORIGIN      
        1 atgacctcat tgtaggcgag gtccaggcgc ttagggaatg gccgtcaggc tcgattacgg
       61 accggcaggt gcctgcgacc ccagaagaga aacgggccaa tccgctcgcc gtcgtcgacc
      121 agctcgaggt gaggaaatcg ccgggccagg ccctgcaggg ccactcgagc ctcgagcaaa
      181 gccagctcgc ggcccaggca gacgtggatg ccgagcccaa aggaaagaac cttccggcca
      241 gtccggccca cgtcgaatac gtccggatcc cggaacgccg ccgggtcccg attggcagcg
      301 ccgtagtggg tgagcacctt ggagtgagcg ggtatcacgg ttcccccgac atcgacgtcc
      361 acggccgtgg tgcggaagag tccgagaagg ggcggatcga atctgaggct ctcgttgatc
      421 gcccggtcca cgtcgacgcg gcccgcgcag aaatcggccc aaagcccgtc gcctgccagc
      481 atgcgccaga cgaaattggt gatgagcgag gtcgtggtct cgtttcccgc gacgaagagc
      541 tgcagcacca ggcgaacggc ttcctcgtcc gtcaccggca cgccgtctcg gcgggcacgg
      601 gcgatgcgcg agatcaggct gccgtcgcca ggacggcgcc gtgtcccgcg cagccgttcc
      661 agcacgtagg cctgcatgcg cgcaagctcc gcgtcgtact gctgcgggtt ctgagaggac
      721 agagcggcga cggaggcgtc ggaccagatc ttgaactgct cgatgtcgtc cgtgggtatg
      781 ccgaatatct cgcagatgat ggtcaccggc agcggaaagg aaagccggtc gtggacgtcc
      841 cagaccgtct cttccgcgac cgcgtcgagc agctcgtcgg cgatggcttc caggcgaggt
      901 cgtagcaggg caatggatct tggcaggaaa tcaggctgga ccagctcgcg gaaaaaggtg
      961 tgctgaggcg ggtcggacac cacgcccatg ggcgcgacga agttgggacc ctgaccgtgg
     1021 ccgctcagaa aacgccctgg atccctcaca atggcgctca cgtcgtcgta gcgggagacg
     1081 acgtagaacg gcgggtcgaa cccgtggtag agccggcagc gcgggccttc gagcagtcgc
     1141 cgggcggaga cggccgggtc ggccatctcc tcgggcccca tcaggtcgag ctcgtcatcc
     1201 atgctgcccg gcaacccgcg tggagtcccc gggctgtaag tctccgatca gctccgcaac
     1261 gatctcgaac gaccgcagcc gggcctggtg atcgaagatc tggccgacga tgatgagctc
     1321 gtcggcaccc gtccgggcga ggaagtcgtt gatggaacgc tctaccgtct cccgtgtgcc
     1381 caccgccgag cacagggaaa accgatcgat gaggtaacgc tgctccggcg tgagggtggc
     1441 gtcgaagtcg tgcaccggcg gcggcagccg ccccggcgca ccctggcgca gctggaggat
     1501 tgcctgcagg gacgagctgc gcaggtaaac ggcttcctcc tccgtgtccg cagcgcagac
     1561 gttgaaaccg agcatgacgt aaggcgcatc gagatgctcc gagggcttga agttcttccg
     1621 gtagacctcg atggcctgat ccatggccgc gggggcgaag tgagaggcaa acgcatacgg
     1681 cagcccgagg gctgcggcga cctccgcacc gaacaggctc gagcctaaga tccataccgg
     1741 caccttggac cccgccccgg gcaccgcatg aacgcgctga ccctcgaccg gatcgcccag
     1801 atagagcatg agctccatga gatcctggcg gaacccgtc
//
LOCUS       DBNHKV010000464         6017 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1483757, whole genome shotgun sequence.
ACCESSION   DBNHKV010000464 DBNHKV010000000
VERSION     DBNHKV010000464.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1483757"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="AC16PP_15215"
     CDS             <1..228
                     /locus_tag="AC16PP_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HIR6483030.1"
                     /translation="DVYLAMTGGQTALFGSEDAVGAGVDRDQAVAAFDGQRPRLKVIL
                     ATDEEIQAHEAVLDFLEQKSGDCVWRQPSGS"
     gene            complement(229..1728)
                     /gene="nhaB"
                     /locus_tag="AC16PP_15220"
     CDS             complement(229..1728)
                     /gene="nhaB"
                     /locus_tag="AC16PP_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019579219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton antiporter NhaB"
                     /protein_id="HIR6483031.1"
                     /translation="MGGLSTASFWENFLGQAPRWYKTTIVGFLIVNPFLFMISEFVTG
                     WILVLEFIFTLAMALKCYPLQPGGLLAIEAVAIGMTSTDTIREEVFNNFPVILLLMFM
                     VAGIYFMKELLLFTFTKIILGVRSKTLVSLLFCAVAAILSAFLDALTVTAVIISVAVG
                     FYSVYHRVASGKSFRDEHEHGADESIGELHREDLESFRAFLRSLLMHGAVGTALGGVC
                     TTVGEPQNLLIAGQVGWEFMEFFLRMAPVTMPVLVVGLTTCVVLEKARAFGYGTDLPG
                     PVRDILERFDQAEAEARSGRDKARLVIQAVAAMLLVVALAMHWAEVGLIGLMVIVIQT
                     AFNGVIEEHNLGRAFEEALPFTSLLVVFFGIVAVIHDQHLFAPVIDYVLTLEVSVQPG
                     MFYIANGVLSAISDNVFVATVYINEVTNAFEEGQIGREHFENLAIAINTGTNLPSVAT
                     PNGQAAFLFLLTSSIAPLVRLSYSKMVFMALPYTIVLGITGWFGVQYLL"
     gene            1990..2841
                     /gene="nudC"
                     /locus_tag="AC16PP_15225"
     CDS             1990..2841
                     /gene="nudC"
                     /locus_tag="AC16PP_15225"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HIR6483032.1"
                     /translation="MWPIYPDEFESLVQAPPGEEDDRDAWYFLCINGQIACVVDRGAP
                     RPITADEFRWLDIEVFSKHYLGRFGNRPCFAMEGRGQLPPDGYLADSLYSWLGRVEPS
                     VFYLAGRAQQVVEWHNTHQYCGRCGAEMQEHAADRAKTCGGCGLICYPRIAPSIIVLV
                     TKGDEMLLARNARWPTGMYSTLAGFVEVGESIEQTVHREVYEEVGIQVDNLRYLGSQS
                     WPFPNSLMLGFHADYAGGEIICNEEEIADAKWFAHDELPSVPPATAISRWLIDDFIEE
                     VGRRSSG"
     gene            complement(2850..3182)
                     /locus_tag="AC16PP_15230"
     CDS             complement(2850..3182)
                     /locus_tag="AC16PP_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HIR6483033.1"
                     /translation="MPHDDLTRRLQSAFHPPAASHMDATFALDLGQLRISFRVRQGRL
                     DFVEGVPADATFYFDTPDEALQLLTGDADVFEAFMEGRFRADGYLMWTFALLSMFRGD
                     GAEPTPTG"
     gene            3314..4354
                     /gene="sohB"
                     /locus_tag="AC16PP_15235"
     CDS             3314..4354
                     /gene="sohB"
                     /locus_tag="AC16PP_15235"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019470684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HIR6483034.1"
                     /translation="MEYLLDYLLFLAQAVTVVVAVLVVLSGVASVSARFQGAERGGHL
                     EVKKLNDRLEELKSGLQQALLDSSTYKKQRKRDAKAAKQRAKVERAEAAKADSEHRKR
                     VFVLDFHGDLQASAVHDLTTELTAVLTLADDRDEIVVKVESPGGLVHGYGLAASQLDR
                     VREHGVPLVVAVDKVAASGGYLMAAVADRVVAAPFAVVGSIGVVAQVPNVHRLLKKHD
                     VDVEVLTAGKFKRTLTVLGENTEEGRKKFIEDLEDTHALFQEFVAARRPQLDMATVAT
                     GETWHGQRAMDLKLVDELCTSDEYLVRSCETADVFSVRWVEHKKPLERIMAKVETGVQ
                     NIVDQLFRRPGP"
     gene            4351..5343
                     /locus_tag="AC16PP_15240"
     CDS             4351..5343
                     /locus_tag="AC16PP_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HIR6483035.1"
                     /translation="MTGFRALLVEKTETGFQRSVVTRRVEDLPPGDLLIDVRYSSLNF
                     KDALSANGNPGVSRNFPHTPGIDAAGVVVESSSEGFAPGDAVVAIGFDLGMNTPGGFG
                     QRIRIPAGWALPLPEGLDLRESMRLGTAGFTAALCVQKLEEAGMTPDGGPVLVAGATG
                     GVGSVAVMLLAKLGYRVTAVTGKPDQHGFLESIGAAEIVSREEARSGSDRPLLPETWG
                     GVVDTVGGETLFNSIKRLRYGASLAACGLVESPQFAATVLPFILRNVNLLGVDSVELP
                     LAEKARVWDKLAGPWKLEGLESLEETLSLDTVSDAIDRILAGAMVGRGVVDLYA"
     gene            complement(5347..>6017)
                     /locus_tag="AC16PP_15245"
     CDS             complement(5347..>6017)
                     /locus_tag="AC16PP_15245"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR6483036.1"
                     /translation="GYGDTLGATLSSLGMALIALPLLILGALGGMRLLVLPLIRKFDA
                     FNEFIFLLALGWCLGLASIAELAGLSFAIGAFVAGVSLATSPIAQYIAENLRPLRDFF
                     LVLFFFSVGAGLDVMLLMDVFFPTLLLALVLVTVKPGTFALLLNWQGEERSTSWEVGY
                     RLGQASEFSLLVSYVALGAGLLGKEAAHVIQGATVLTLLLSSYAVIFRYPSPIAISAR
                     LRRD"
BASE COUNT         1145 a         1811 c         2018 g         1043 t
ORIGIN      
        1 gacgtttatc tagccatgac gggcgggcag acggctctgt tcggctcgga ggacgccgtg
       61 ggggccggcg tcgatcggga tcaggcggtc gcggcctttg acggccagcg tccacggttg
      121 aaggtcatat tggctaccga cgaagagatc caggctcacg aagccgtgct ggacttcctc
      181 gagcagaaat ctggcgattg cgtctggcgc cagccatccg ggtcctgatc agagcaggta
      241 ctgcaccccg aaccagcccg tgatccccag gacgatggtg taggggagcg ccatgaaaac
      301 catcttgctg tacgacaacc gcaccagggg ggcgatggac gacgtcagca ggaacagaaa
      361 ggcggcctgg ccgttggggg tcgctacgct cggcaggttg gtgccggtgt tgatcgcgat
      421 cgccaggttt tcgaagtgct cgcggccgat ctggccttcc tcgaaagcgt tcgtcacctc
      481 gttgatgtag acggtcgcga cgaagacgtt gtcgctgatg gcggatagca cgccgttggc
      541 gatgtagaac atgccgggct gtacggacac ctctagggtg aggacgtagt cgatcaccgg
      601 ggcgaacagg tgctggtcgt ggatgacggc gactatcccg aagaacacga cgagcagaga
      661 cgtgaaaggc agcgcctcct cgaaggcgcg gccgagattg tgctcctcga tgacgccgtt
      721 gaaagcagtc tggatcacga tgaccatcag cccgatcagc ccgacctcgg cccaatgcat
      781 ggccagcgcg acgacgagga gcattgcggc caccgcctga atcactaagc gggccttgtc
      841 gcggccgctg cgtgcttcag cttcggcctg gtcgaagcgc tcgagaatgt cgcgcaccgg
      901 gccgggcagg tcggttccgt agccgaaagc tctggctttt tctaacacga cgcaggtagt
      961 cagcccgacc accagaaccg gcatcgtgac gggtgccatg cgcaggaaga actccatgaa
     1021 ctcccatccc acctggccgg cgatcagcag gttctgtggt tcgcccaccg tggtgcagac
     1081 accgcccaat gcggttccca cggcgccgtg catcagcagg ctgcgcagga aggcgcggaa
     1141 cgactccagg tcctcacgat gcagctcacc gattgattcg tccgcaccgt gctcgtgctc
     1201 atccctgaag gactttccgg aggcgaccct gtggtagacg ctgtaaaagc ccacggcaac
     1261 gctgatgatc accgccgtca ccgtcagcgc gtcgagaaag gcggacagga tcgcggcgac
     1321 ggcgcagaac agtaaggaga cgagggtctt tgagcgaacc ccgagaatga tcttggtgaa
     1381 ggtgaagagc agcagctcct tcatgaagta aatgcccgcc accatgaaca tgaggagcag
     1441 gatgacggga aagttgttga agacttcctc gcgtatggtg tccgtggacg tcatgccgat
     1501 cgccacggct tcgatagcga gcagaccgcc gggttgcagc ggataacatt tcagggccat
     1561 ggccagggtg aagatgaact cgaggaccag aatccacccg gtgacgaact cgctgatcat
     1621 gaaaaggaac ggattgacga tcaggaaacc gacgatggtc gttttgtacc accgtggtgc
     1681 ctggccgaga aaattttccc agaagctggc cgtggatagt ccgcccatct gcttttgccc
     1741 ggcgcgccgg gaaaactgct ccctgtaatt gcctgcgagg tggccgcagc aaaccagcag
     1801 acctttgcct ccccggccgg tcctcccttc cggccaaacc ggtggtcgag cgcacgggtg
     1861 tcagatcacg cttcgagaac attatccggc tcggcagggg agttcaatac gatcgtggcc
     1921 ggaccctgaa caggccggtg ggtgtgttat cgtttcggct catcgtcgat accacgagtc
     1981 ttgcccgcca tgtggcccat ctaccccgat gagttcgagt ctctcgttca agcccctccc
     2041 ggtgaggagg acgatcggga cgcctggtac ttcctttgca tcaacggtca gatcgcctgc
     2101 gtcgtggacc gcggcgcccc ccggccgatc actgccgatg agttccggtg gctggacatc
     2161 gaggtcttct ccaagcacta ccttggccgc ttcggcaatc gcccctgctt cgctatggag
     2221 gggcgcggcc agctgccgcc ggacggttac ctcgcggaca gcctttactc gtggctaggg
     2281 cgcgtagagc cgtcggtgtt ctacctggcg ggtcgggcgc agcaggtggt ggagtggcac
     2341 aacacccatc agtactgcgg gcgctgcggc gcggagatgc aggaacacgc cgccgatcgt
     2401 gccaagacgt gcggcggatg cgggctgatc tgctatccga ggatcgcgcc gagcatcatc
     2461 gttctggtga cgaaaggcga cgagatgctg ctggctagaa acgcgcgttg gccgacgggc
     2521 atgtacagca cgctggcggg gttcgtcgag gtgggcgagt ccatcgagca gacggtgcat
     2581 cgggaggtct acgaagaggt cggaattcag gtagacaatc ttcgctacct tggcagtcag
     2641 agctggccgt ttcccaactc gctcatgctg ggctttcacg cggactacgc aggcggcgag
     2701 atcatctgca acgaggagga gatagccgac gccaagtggt ttgcccacga cgagctgccc
     2761 agcgtgcccc ctgctaccgc catctcccga tggctcatcg atgacttcat cgaggaagtc
     2821 gggcgccgca gctccggctg atgcggcctt cagccggttg gtgtgggctc tgccccgtcc
     2881 ccgcgaaaca tgctcagcag ggcaaaagtc cacatcaggt agccgtccgc gcgaaagcgg
     2941 ccctccatga aggcctcgaa cacgtccgca tcgcccgtga gcagctgcag cgcctcatcc
     3001 ggcgtgtcga agtaaaaggt cgcatctgcc gggacgccct ccacgaaatc gagccggccc
     3061 tgacggacgc gaaaactaat gcggagctgg cccagatcga gggcaaacgt ggcgtccatg
     3121 tgggaagccg ccggcggatg aaacgcgctc tgcagtcgcc gggtcaggtc gtcgtggggc
     3181 atcgttgaat cagacacgca tgtgcggatg agatcaagcc gtgcgcatgt ggctgcaggc
     3241 gcggcggtgg acgcccgttg acgcagccag tacattagcg cgcccttgct ttgcgtaaaa
     3301 attccggggt ggcatggagt atctgctcga ctatctgctc tttctggccc aggccgtcac
     3361 ggtcgtcgtg gccgttctcg tcgtgctttc cggcgtcgct tccgtgagcg ccaggttcca
     3421 aggtgcggag cggggcggtc atctcgaggt caagaagctc aatgaccggc tcgaggagct
     3481 gaagtcgggg ctgcagcagg ctctgctcga cagctcgacc tacaaaaagc agcgcaagcg
     3541 ggatgccaag gccgccaagc agcgagccaa agtcgagagg gccgaggctg ccaaggccga
     3601 tagcgagcat cgcaagcggg tcttcgtcct cgacttccac ggcgatctcc aggcgagcgc
     3661 cgtccatgat ctcacgacgg agctgaccgc tgtgctcacg ctcgccgatg accgtgacga
     3721 gatcgtggtc aaggtggaaa gccccggcgg gctggtgcac ggatacggcc tggcggcttc
     3781 tcagctcgac cgggtgcgtg agcacggggt gccgctcgtc gtcgcggtgg acaaggtcgc
     3841 cgcgagcggt ggttatctga tggctgccgt ggctgacagg gtggtcgcgg ccccgtttgc
     3901 ggtcgtggga tccatcggcg tggtggccca ggtacccaac gtccatcggc ttctgaaaaa
     3961 gcacgacgtg gacgtggagg tgctcacggc cggcaagttc aagcgcacgt tgaccgtgct
     4021 tggtgagaat acggaagagg gccggaagaa gttcatcgag gacctcgagg acacccacgc
     4081 gctgtttcag gaattcgtgg ccgcgcggcg tccgcagctg gacatggcca ccgtggccac
     4141 cggagagacc tggcacgggc agcgggccat ggacctcaag ctggttgacg agctttgcac
     4201 cagcgacgag tacctggtgc gctcgtgcga gacggcggat gtgttcagcg tgcgctgggt
     4261 ggagcacaag aagccgttgg aacgaatcat ggccaaagtg gagaccggcg ttcagaacat
     4321 cgtcgatcag ctgttcagga ggcccggacc atgacgggtt tccgtgccct gctggtggag
     4381 aagaccgaaa ccggtttcca gcgctcggtc gtcacgcgcc gcgtggagga tttgccgccc
     4441 ggcgatctgc tgatcgacgt tcgctactcc tcgctcaact tcaaagatgc gctttccgcc
     4501 aacggcaatc cgggcgtgag ccgtaatttt ccgcacaccc cgggcatcga cgctgcgggc
     4561 gtggtcgtgg aatcgtccag cgagggcttc gccccgggcg acgccgtggt cgccatcggt
     4621 ttcgacctcg gcatgaacac gccgggcggt ttcggccagc gcatccggat tccggcggga
     4681 tgggccctgc cgctaccgga gggattggat cttcgcgaat ccatgcgcct cggaacggcc
     4741 ggtttcacgg ccgccctgtg cgtgcagaag ctcgaagagg cgggcatgac gccggatggc
     4801 ggtccggtgc tggtggccgg cgccaccggt ggcgtgggat ccgtcgccgt catgctgctg
     4861 gcaaagctcg gctatcgggt caccgcggtg acgggcaaac ccgatcagca cggctttctc
     4921 gagtccatcg gcgctgcaga gatcgtgtcc cgggaagaag cgcgctccgg gtcggacagg
     4981 ccgttgctgc cggaaacctg gggtggggtg gtggacaccg ttggcggcga gacgctcttc
     5041 aactcgatca agcgcctgcg ttacggggcg agcctggccg cttgcggtct cgtggagtcg
     5101 ccgcagttcg cggccaccgt gctgcccttc atcctgcgca acgtcaattt gctcggcgtg
     5161 gattcggttg agctgcccct ggcggagaaa gcgagggtct gggacaagct ggcgggcccc
     5221 tggaagctcg aaggtttaga gtccctcgag gagacgctct ctctggatac cgtctcggat
     5281 gccatcgacc ggatactcgc cggtgccatg gtgggacgtg gcgtggtgga cctgtacgcc
     5341 tgaaactcag tccctgcgca gccgggccga tatggcaatc gggctgggat atctgaagat
     5401 caccgcatag ctcgacagca gcagggtgag cacggtcgcg ccctggatga cgtgggcggc
     5461 ctccttcccc agcagcccgg cgccaagcgc gacgtagctc accagcaggg agaactccga
     5521 ggcctgtccc aggcgatagc cgacctccca cgacgtggaa cgctcctcgc cctgccagtt
     5581 gagcagcagg gcgaacgtgc ccggcttgac ggtaacgagg accagcgcca gcagcagcgt
     5641 ggggaagaaa acgtccatca ggagcatgac gtcgagaccg gctccaaccg agaagaagaa
     5701 gagaaccaga aagaagtctc gcagtgggcg caggttctcg gcgatgtact gcgcgatggg
     5761 gctggtggcc agggaaacac cggccacgaa agcgccgatg gcaaacgaca ggccggcaag
     5821 ctccgcgatg ctggcaaggc ccaggcacca gcccagcgcc agcagaaaaa tgaactcgtt
     5881 gaaggcgtcg aattttctga tcagcggcaa taccagcagg cgcatgccgc cgagcgcgcc
     5941 gaggatgagc agcggtagcg cgatgagcgc catcccgagg ctcgacagcg tggcgcccaa
     6001 cgtatcgccg tagcccg
//
LOCUS       DBNHKV010000465         3907 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_278593, whole genome shotgun sequence.
ACCESSION   DBNHKV010000465 DBNHKV010000000
VERSION     DBNHKV010000465.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_278593"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="AC16PP_15250"
     CDS             complement(<1..749)
                     /locus_tag="AC16PP_15250"
                     /inference="COORDINATES: protein motif:HMM:NF024375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6483037.1"
                     /translation="MNTGYNPEFNPTGIEGGIDTNALPWIDLPQAPGVSIKPLRASSE
                     SGMFSTVVRVADGSELSSIVYLGAMDMIVLSGRLTYPSGPMSGTLEPGTWGYVPANAR
                     VERFIAEQDTEFLANFYGPVAFLADDGRTVSSLLTSLDIQAAARDRGINLVPNTLAEC
                     MQERPPAYQGPVEPLAIAGSDSKALVVAAEGVAADAGRAVHPHFIDTRAVPWIVNPDT
                     PDLGLKILRVSEETGITSLIVRHNGVAGPHY"
     gene            complement(819..1514)
                     /locus_tag="AC16PP_15255"
     CDS             complement(819..1514)
                     /locus_tag="AC16PP_15255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6483038.1"
                     /translation="MTDVLITGAARGIGFELARQYAEAGDRVYATSRNPDGAAQLSAL
                     AADFPDNVSVHRMDVGDMGSVQGCAAELNAAPIDVLINNAGVWGGLETQTFQNMDYEN
                     WAFEFNVMAMGPFRVIQTFLPNVLAGRDKKIVTLSSQVAAHVYDHVIGYSYASAKAAV
                     NRLMTALATELQDQGIIVVPMHPGWVKTDMAGDVADIYPSESAEGVRRVIAGMTMKDT
                     GKFLKWTGDIHPW"
     gene            complement(1524..2909)
                     /locus_tag="AC16PP_15260"
     CDS             complement(1524..2909)
                     /locus_tag="AC16PP_15260"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR6483039.1"
                     /translation="MAESQPKPGEARCPGISVQDLMDRDSRTVPAYLREHSYAFLGDE
                     DIPFARYTSRDWFDKEIEHMWPRTWQWACREEHLPAPGDYYVYDVGPYSVIVLRDSSG
                     AIRAYRNACMHRGTKLKPSFSTGSADSIRCPYHGWQWNLEGELQSFPCEWDFPHVDPD
                     AVGLSSVRAELWGGFVFINLDPEAVPLREYLHPLPEHVDSSVFEQRYVAIHIQKELNC
                     NWKIASEAFLEAYHVMETHPQLMRTNGDANTQYDFYGDHVNRLINVAGSPSAYLEEPI
                     TEQEVLDLFLVGDREDIGEALVVPEGGTARQVMAEHFRESVNASGGADLSEVCDSEIV
                     DTIAYFVFPNQQFFSGVSFPIVYRFRPLGMSHDRSLFDFVILKTRPNDGSPAPTAEPV
                     RLTADESYTLVPGMDPYVGHVFDQDTGNVQAAQEGAMATDKAGATLGNYQEIRIRHFH
                     QTLGKYVAGEL"
     gene            complement(2983..3744)
                     /locus_tag="AC16PP_15265"
     CDS             complement(2983..3744)
                     /locus_tag="AC16PP_15265"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF048554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6483040.1"
                     /translation="MRPIAGMSILVTGASSGLGAGAAAYFAERGARVTISARREDRLA
                     AVKEAIGDAAAMVVGDVTSAADRQRMIDEAVRHGGGLDALISNAGNMYRGPITDLDEQ
                     ALLDIFHSNVVAGMLLTGLAVPELEKRQGAVIFVGSIHTMRAFPGASPYAATKGALQT
                     LTRVLAAELGERGIRVNCVIPGAVPTEIYQRAGLLSDEDARARIAGMASMHPLGRVGT
                     EEELAEAMAYLIQAEWTTGAELVVDGGLGLGATNA"
     gene            complement(3741..>3907)
                     /locus_tag="AC16PP_15270"
     CDS             complement(3741..>3907)
                     /locus_tag="AC16PP_15270"
                     /inference="COORDINATES: protein motif:HMM:NF018038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HIR6483041.1"
                     /translation="RVPDGDWHPISWAHEAAAGRVVYSALGHDGRSLGDPHHARFIRQ
                     AVAWLLGEDE"
BASE COUNT          624 a         1285 c         1290 g          708 t
ORIGIN      
        1 tggtagtggg gcccggcgac gccgttgtgc cgcacgatga gcgacgtgat gccggtttcc
       61 tcgctgaccc ggagtatctt caggcccagg tcgggcgtgt ccggattgac gatccaggga
      121 accgctcggg tgtcgatgaa gtgcgggtgc accgcacggc ccgcatctgc agcgacgccc
      181 tcggcggcaa ccaccagtgc ctttgagtcg ctgccggcga tggccagtgg ctccacgggg
      241 ccctgataag cgggcggccg ctcctgcatg cactccgcca gggtgttggg caccaggttg
      301 atgccccggt cgcgcgcggc cgcctggatg tccaggctgg tcagcaggct cgacaccgtg
      361 cgcccgtcgt ccgcgagaaa ggcaaccgga ccgtagaagt tcgccagaaa ctccgtgtct
      421 tgctccgcaa tgaaccgttc cacccgggca ttcgcgggca cgtagcccca ggttccgggc
      481 tccagggtcc ccgacatggg tcccgagggg taggtgagcc ggcccgacag gacgatcatg
      541 tccatggcgc cgaggtagac gatgctcgac agctcgctgc catcggctac cctgaccacg
      601 gtggaaaaca tgcccgactc gctgctggcc cggagcggct tgatcgatac cccgggtgcc
      661 tggggcaggt cgatccaggg aagcgcgttg gtgtcgatgc cgccttcgat tccggtggga
      721 ttgaactccg ggttgtagcc tgtattcatt ggtttagcct tccctgatct gtttgcattg
      781 cgtgtgtgcc ggtcgcccgg cagaaaccgt tcgtgtcgct accacgggtg aatgtcgccg
      841 gtccatttca ggaacttgcc cgtgtccttc atggtcatcc cggcgatgac ccggcgaacg
      901 ccctcggcgc tttccgaagg atagatgtct gcgacgtcgc ccgccatgtc ggttttcacc
      961 caccccgggt gcatgggaac gacgatgatg ccctggtcct ggagttcggt ggccagggcc
     1021 gtcatcagcc ggttcacggc ggccttggcg ctggcgtagg agtagccgat gacgtgatcg
     1081 tagacgtggg cggctacctg gctggagagc gtcacgatct tcttgtcgcg cccggcgagc
     1141 acgttcggca gaaaggtctg gatgacccgg aaggggccca tggccatgac gttgaactcg
     1201 aacgcccagt tctcgtagtc catgttctga aaagtctggg tctccagccc gccccagacg
     1261 ccggcgttgt tgatcagcac gtcgatgggc gctgcgttca gctcggcggc gcagccctgc
     1321 accgacccca tgtcgcccac gtccattcgg tgcacgctga cgttgtccgg gaaatcggcc
     1381 gccagcgcgc tgagttgggc ggcgccgtcg ggattccggc tggtggcgta gacccggtcc
     1441 ccggcctccg cgtattgcct ggccagctcg aaaccgatac cgcgggctgc cccggtgatc
     1501 agaacgtctg tcatcgtgtg ctcctaaagc tcgccagcaa cgtacttgcc gagagtctgg
     1561 tgaaagtgac ggatgcgtat ttcctgataa ttgcccaggg tcgcgccggc cttgtcggtg
     1621 gccatggcac cctcctgggc tgcctggacg ttgccggtgt cctgatcgaa aacgtggccg
     1681 acatagggat ccatgcccgg taccagcgtg tacgactcgt ccgccgtcaa ccgcacgggc
     1741 tcggccgtgg gtgctggaga gccgtcgttg ggccgcgtct tgaggatgac gaagtcgaac
     1801 aggctgcggt cgtggctcat gccgagcggc cggaagcggt aaacgatggg aaacgatacc
     1861 ccggagaaga actgctggtt ggggaagacg aagtaggcga tggtgtccac gatctccgaa
     1921 tcgcacactt cggagagatc cgctcccccg gacgcgttca cgctctctct gaaatgctcg
     1981 gccatgacct ggcgagcggt gccgccctcg ggcaccacca gcgcctcgcc gatatcctca
     2041 cgatccccca ccagaaacag atccagcacc tcctgctcgg ttatcggctc ctcgaggtaa
     2101 gcgctcggcg agccggcgac gttgatcagc cggttgacgt gatcgccgta gaagtcgtac
     2161 tgggtgttcg cgtcgccgtt ggtgcgcatg agctgagggt gggtttccat cacgtgatag
     2221 gcttctaaaa acgcctccga ggcgatcttc cagttgcagt tcagctcttt ctgtatgtgg
     2281 atggccacgt agcgttgctc gaagacgctg ctgtccacgt gctcgggcag gggatgaagg
     2341 tactcccgta gcggaacggc ctcgggatcc aggttgatga acacgaagcc gccccagagt
     2401 tcggcccgga cggaggacag gcccacggca tcgggatcga cgtgggggaa atcccactcg
     2461 cagggaaacg attgcagctc gccctccagg ttccactgcc agccgtgata agggcagcgg
     2521 atggagtcag ccgaacccgt gctgaacgag ggcttgagct tcgtcccgcg atgcatgcag
     2581 gcgttgcggt atgcccggat cgcgccgctg ctgtcccgca acacgatgac cgagtacggg
     2641 ccgacgtcgt aaacgtaata atcaccgggg gccggcaggt gctcctcgcg acacgcccac
     2701 tgccacgtcc tcggccacat gtgctcgatc tctttgtcga accagtcgcg cgacgtgtag
     2761 cgggcaaaag gaatgtcctc gtcgccgaga aacgcgtagc tgtgctcgcg cagataggcg
     2821 ggcaccgttc ggctgtccct gtccatcagg tcctgtaccg aaatgcccgg gcagcgtgcc
     2881 tcacccggct tgggctggct ctcggccatc gcctccccct gatttgtcct tatccccagc
     2941 ggacatttta agtcaccggc gacgatacgc caccactgcc cgctacgcgt tggtggcccc
     3001 tagccccagc ccgccgtcca ccaccagctc ggcccccgtg gtccactccg cctgaatcag
     3061 gtaagccatg gcctcggcga gctcctcctc ggtgccgacc cggccgaggg ggtgcatgga
     3121 ggccatcccc gcgatgcgag cccgcgcgtc ctcgtccgac agcaggcccg cacgctgata
     3181 gatctcggtg ggcacggcgc cggggatcac gcaattcacc cggatgccgc gctcgccgag
     3241 ctcggcggcc agaaccctgg tcagggtctg cagcgcgccc ttcgtcgccg cgtagggcga
     3301 agcgccgggg aatgccctca tggtgtggat gctgccgacg aagatcaccg cgccctggcg
     3361 cttttccagc tccggtacgg cgaggcccgt gagcagcatc ccggcgacga cgttgctgtg
     3421 aaagatgtcc aggagcgcct gctcgtcgag gtcggtgatg ggcccgcggt acatgttgcc
     3481 ggcgttgctg atcagcgcgt cgagcccccc gccgtggcgg accgcttcgt cgatcatgcg
     3541 ctgccggtcc gctgcgctgg tcacgtcgcc gacgaccatg gcggctgcgt cgccgatggc
     3601 ctccttcacc gccgcgagcc ggtcctcccg gcgggcggag atggtgaccc tggccccccg
     3661 ctcggcgaag taagccgccg caccggcgcc gagcccggag ctggcgccgg tgacgaggat
     3721 cgacatgccg gcgatgggcc tcactcgtcc tctcccagca gccaggccac cgcctggcgg
     3781 atgaaccggg catgatgagg gtcgccaagg gatcgtccgt cgtggccgag cgccgagtag
     3841 acgaccctgc ccgcggcggc ctcgtgcgcc cagctgatgg ggtgccagtc gccgtcggga
     3901 acccggc
//
LOCUS       DBNHKV010000466         6566 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1484096, whole genome shotgun sequence.
ACCESSION   DBNHKV010000466 DBNHKV010000000
VERSION     DBNHKV010000466.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6566)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1484096"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..801
                     /gene="tsaB"
                     /locus_tag="AC16PP_15275"
     CDS             118..801
                     /gene="tsaB"
                     /locus_tag="AC16PP_15275"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR6483042.1"
                     /translation="MTTILALETSGSLCSVALICETGSFENTRNVERMHNQVLLGMLD
                     DLFEQAAVAPAGVDVVAFGAGPGSFTGVRIAAAVAQGIGVGIGGKVVPVSSSLARAAV
                     AFREHPDDTAVITLIRSRRDAYYLAGYTRSSGKIAISFADVLVTAWPDGLWDGQRLAV
                     GDLPDWWSSDRPETLNFGGEALVTAPVIAELGLAAAHAGGAFDPALGLPVYLKGDSPW
                     KPVDSGGSG"
     gene            845..1567
                     /locus_tag="AC16PP_15280"
     CDS             845..1567
                     /locus_tag="AC16PP_15280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016340.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6483043.1"
                     /translation="MRLYVSARQRDEIRRYQDLFDIVVTSGPPVDEDGPFLYWNGERL
                     QLFSGHDGKPAAVCDEEIERRATFSSELARACGVTRSRQPSVLDALAGWGVDGMSLAA
                     LGSSLVMVEQNDAMWALLDSFLAHHPGQRPELIHDDAWAVLDGSRSFDVVYLDPMYAR
                     RRKGALPGKSMQLLALVCGPDQRSAADWIDRARPCAAGRVVLKRRLRDPLVGRPDWQI
                     KGHSVRYDVYRGRGDQAPLSTA"
     gene            complement(1543..3927)
                     /gene="plsB"
                     /locus_tag="AC16PP_15285"
     CDS             complement(1543..3927)
                     /gene="plsB"
                     /locus_tag="AC16PP_15285"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF003441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HIR6483044.1"
                     /translation="MRQLRRLGYLVSRWLIRSIIRPRLIGDPPDPPREGRPVYLYSLE
                     NRSLSDLIVLDLLNEANHLPGPLDPVTLGSHTEGRRVFFLNRASYGLFQRNVMSTPSE
                     RLIRVLTHAGEANDAVIVPVTVLWGRAPSRQGSLFRSLLAEDWAVTTRLKRILNLVVS
                     RKEIYVHYGTPISIGELAEDDLDVNRMVRRAARLLRVQFRNQRVSAIGPDFSHQRTLV
                     NQILRSRAVTDAIAAADPEEKPERMERRARKYAMSIVSNMSYPTIRVLERLLTWFWNR
                     IYDGLKLHGLERVMEIAETHTPIYTPSHRSHVDYLLLSYLLHKNGLMIPHIAAGDNMN
                     LPFVGHLLRRGGAFFMRRSFRDDPVYTAVFSEYLYQVYRRGHCVEFFPEGGRTRTGRL
                     LPARMGLLKMTLEHHRRGVPRPLAIVPVYLGYEKLIEGGSYLDELRGSEKQRESIGDI
                     FRSIKLIRQNFGQVNVNFAKPIDLNEWSNAHPDVPENELPARLGQEILERINESAAIN
                     PINLVALVTLATPKLAIDEMLLIDQIGCYLELLRAEAPQHDYTVTDQSPAEVVGYVER
                     LGMLTREEAEFGDILCHDPFAAVLMTWYRNNVTHTLALPSLIACMVINRRRPMGKAAL
                     TRMVDTVFPYLAAELHMVGDHAAIDRWLKHLVRANLLELHPAGGYCAPSVRSQSRYRL
                     HLLSRLIMQTLERLYIVVALIGQPGITVRTRSDLETQSQQVARKMSRIHGLNAPEFFD
                     SRLFNGFIDQLIANDIVTESTDGSLTYRPIISNVIKAAESIIDAEFRYAVLRGA"
     gene            complement(3959..5863)
                     /gene="gspD"
                     /locus_tag="AC16PP_15290"
     CDS             complement(3959..5863)
                     /gene="gspD"
                     /locus_tag="AC16PP_15290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HIR6483045.1"
                     /translation="MNKIFATTLTYAMVLLLLLPTSLPAQGQEQTWKINIKNADIQEF
                     VAQVAAITGKTFVIDPRLKGNVTVISNTSMDEEAVYALFLSVLRVHNFTATPSGDVIR
                     IQQNATGKQTPGVSGELGEVPPEELVTRVVSAQNVESAELVKILRPLIPQYGHIAAVA
                     VPNVVIISDHADNILRLMRMIEQIDVADEEEIVVVPLKEAWVGTIVALLEKVAPDQMG
                     RNAQGPQRIQIIANERNNSLVLKGKPRPVAEVLKIIEKLDQPATTAGATQVIYLKHAD
                     AVKVSEILNSVIEDRSRGEDGATERPNIQADETLNAIVVRADPGTMNEILEILESLDV
                     RRTQVLIEAAVVEVTITNASDIGVEMAIVDESGQTVPLASTSLDGAINSLIGNIIGDD
                     GEIDPVSGLASITSPTLAAARIDRGDVSFGAIVRALATNSNANLLSTPSILTLDNQEA
                     KIVVGNEVPFRTGSFTTSGNDSSNPFTTVQREDVGLQLTVTPHVHEGTSVRLEVAQEI
                     TNVINAPIGNDGFSDVVTSKRSIETTILAEDRQTVVLGGLIQDDITDTVKKVPLLGDI
                     PGLGALFRSNSKSRSKSNLLVFLRPTVIRNKEDSDTATNRKYTDIWEVEISSGGGKSD
                     ITDNFRGRKD"
     gene            complement(5881..>6566)
                     /locus_tag="AC16PP_15295"
     CDS             complement(5881..>6566)
                     /locus_tag="AC16PP_15295"
                     /inference="COORDINATES: protein motif:HMM:NF022800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HIR6483046.1"
                     /translation="PAATQQTAMAVATRLPLELQGVFVADASEDSAAIVAQKGRPGML
                     YVVGQKMPGNAELVEVHADHIVLRRAGVLETLKFPTSIGQFVAAPDDLEDLADQALPF
                     DMPTDVQEDADDDRDERGRKRPTSEALAEYRERIEADPQGVLADLGISAVNEGDASGY
                     RLDDLNDSPFLSQTGLQPGDVILSVNGRPVGDINQDQQELDNMLAQGSARLEVQRGTR
                     RFFVTASLP"
BASE COUNT         1094 a         1905 c         2206 g         1361 t
ORIGIN      
        1 gcggccatgt tttgtaccgt actgatatta aagggttatt ttgagtagaa gccccgtttt
       61 acgggccttt cgagggggca gaatgctaca gcgcgggagc cgagagggtt tgtttggatg
      121 acaacgatac tggctctgga aacttcggga tcgctctgct cggtcgcgtt gatctgcgag
      181 acgggctctt tcgagaatac acgcaacgtc gagcgcatgc acaatcaggt gctgctggga
      241 atgctcgatg acctgttcga gcaggccgcg gtggcgccgg ccggtgtcga cgtggttgcg
      301 ttcggcgcgg gtccgggttc gttcactggt gtgagaatcg ccgcggctgt ggcccagggc
      361 atcggtgtcg ggataggcgg caaggtcgtt cccgtttcct cgtcgcttgc ccgagcagcc
      421 gttgcgtttc gagagcatcc tgatgatacg gcggtaatca ctctgatccg gtcccggcgg
      481 gatgcctatt accttgccgg ctacacccga tcgtctggaa agatcgcaat ttcgtttgcg
      541 gacgtgctgg tgacggcgtg gccagatggc ttgtgggatg gccagcggct ggccgttggg
      601 gatctgcccg actggtggag ttcggacagg cccgaaacgc tgaatttcgg tggcgaggcg
      661 ctggtgacgg cgccggtgat cgccgaactg ggcctcgcgg cagctcatgc gggcggagcg
      721 ttcgatccgg cccttgggtt gcccgtctac ttgaagggcg attcgccctg gaagcccgtc
      781 gatagcggcg ggtccgggtg acggccttca gccgtcagcc agacgtcaac tgaggcgcta
      841 cccgatgcgt ctgtatgtat ccgcccgaca gcgagacgag atccgccggt accaggatct
      901 gttcgatatc gtggtaacga gtggaccacc cgtcgacgag gacggtcctt ttctgtactg
      961 gaacggcgaa cgtctgcaat tgttcagcgg gcatgatggc aagccggcgg ccgtctgcga
     1021 cgaagagatc gaaaggcgcg cgaccttcag cagcgagctg gcacgggcat gcggggtgac
     1081 gcgctcccgg caaccaagcg tgctcgacgc cctggctggc tggggcgtcg atgggatgtc
     1141 ccttgctgct ctcggcagct cgctggtgat ggtcgagcag aacgatgcca tgtgggcgct
     1201 tctggacagc tttctggcgc accacccggg gcaacggccc gaactgattc acgacgacgc
     1261 gtgggccgtt cttgacggca gccgatcgtt cgacgtcgtg tatctggatc cgatgtacgc
     1321 ccgtcgccgc aaaggcgcgc tgccgggaaa gtcgatgcag ctgctggcgc tggtttgcgg
     1381 acccgaccag cgatcggccg ccgactggat cgatcgcgca cgaccttgcg cggcggggcg
     1441 tgtggtgttg aagcgtcgtc ttcgcgatcc gctcgtcggt cggcccgact ggcagatcaa
     1501 gggtcacagc gtgcgatacg acgtctatcg aggtcggggg gatcaggcgc ccctgagcac
     1561 ggcatagcgg aactcggcat cgatgatgct ttccgctgcc ttgatcacgt tgctgatgat
     1621 tgggcggtag gtgaggcttc catccgtcga ctcggtaacg atgtcgttcg cgatcagctg
     1681 gtcgatgaag ccgttgaaca acctcgaatc aaaaaattcc ggcgcgttca gcccgtggat
     1741 gcgggacatt ttgcgtgcca cctgctggct ctgggtttcc aggtcgctgc gcgtacggac
     1801 cgtgatgccc ggctgaccga tgagtgctac cacgatgtag agccgttcaa gggtctgcat
     1861 gatcagtcgc gaaagcaggt gcagtcggta gcgggactgg ctcctgacgg atggggcgca
     1921 atagccgccg gccggatgaa gctccagcag atttgcgcga accaggtgct tcagccaacg
     1981 atcgatggcc gcatggtctc cgaccatgtg gagctcggcg gcaagatatg ggaataccgt
     2041 gtccaccatg cgtgtcagcg ccgctttgcc cattggccgg cgccgattga tgaccatgca
     2101 ggcaatcagc gaaggaagcg ccagggtgtg ggtgacgttg ttccggtacc aggtcatgag
     2161 cacggccgca aacgggtcgt gacagaggat gtccccgaat tcggcctcct cccgggtcag
     2221 catgcccagg cgctcgacgt agccgaccac ctcggccggg ctctggtcgg tgacggtgta
     2281 gtcgtgctgg ggcgcctccg cccggagcag ttcgaggtaa cagccgatct ggtcgatcag
     2341 gagcatctcg tcgatggcga gcttgggcgt agcgagcgtg accagcgcca cgaggttgat
     2401 ggggttgatc gcggcgctct cgttgatcct ctccagaatc tcctggccga gccttgcggg
     2461 aagctcgttt tcgggaacgt ccgggtgagc gttgctccac tcgttgaggt cgatgggttt
     2521 cgcgaagttg acgttcacct gcccgaagtt ctgccggatc agcttgatgc tgcggaatat
     2581 gtccccgatg gactcgcgct gcttttccga gccgcgtaac tcgtccagat aactgccgcc
     2641 ctcgatcagc ttctcgtagc cgaggtagac gggcacgatg gccaggggcc ggggtacgcc
     2701 acgtcgatgg tgttccaggg tcatcttgag cagtcccatg cgagcgggca gcaatcgtcc
     2761 ggttcgcgtt cgtccaccct cgggaaaaaa ttccacgcag tgaccgcgcc ggtaaacctg
     2821 atagaggtac tccgagaaaa cggccgtgta tacgggatcg tcacggaagc tgcgtcgcat
     2881 gaagaaggct cctccgcgcc gcagcagatg acccacgaaa ggaaggttca tgttatcgcc
     2941 ggctgcgatg tgcggaatca tcaggccgtt cttgtgcagg aggtatgaaa gcagaagata
     3001 gtccacatga cttcggtgag atggggtgta gatgggagta tgtgtctcgg cgatctccat
     3061 cacccgctct agcccgtgca gcttcaaccc gtcataaatt cgattccaga accaggtgag
     3121 cagacgctcg agtaccctga tggtgggata cgacatgttc gagacgatgc tcatcgcgta
     3181 cttgcgtgct cggcgttcca tgcgctccgg cttctcttcg gggtctgccg ccgctatggc
     3241 gtcggttacc gcgcggctgc gcagtatctg gttgacgagc gtgcgctgat gggaaaagtc
     3301 gggcccgatg gcggaaacgc gctggttacg gaactgcact cgaagcaggc gcgcggcccg
     3361 gcggaccatc cgattgacat cgaggtcgtc ttcggcgagt tccccgatcg agatgggggt
     3421 gccgtagtga acgtatattt ccttgcggct gacgacgagg ttcaggatgc gcttcaggcg
     3481 cgtggttacc gcccagtcct cggccagcag tgagcggaac aaagagccct gacgcgacgg
     3541 cgcgcggccc cagaggacgg taacgggcac gattaccgcg tcgttcgctt cgcccgcgtg
     3601 ggtgagcacg cggatcagcc gttccgacgg cgtgctcatg acgttgcgtt ggaataggcc
     3661 gtagctcgcc cggttgagaa agaacacgcg gcggccttcg gtgtggcttc ccagagtcac
     3721 cgggtccagg gggcctggca ggtggttggc ctcgttgagg aggtcgagga cgatcagatc
     3781 gctcagcgac cggttttcca gcgagtacag gtagacgggt cggccttccc gcggcggatc
     3841 gggagggtcg ccgatgaggc ggggacgtat gatcgaccgg atgagccagc ggctgaccag
     3901 gtagccgagc cgtctgagtt gtcgcatgga ccgcctttag gtgttggggg cacgcatgct
     3961 aatccttgcg ccccctgaag ttatccgtga tgtcggattt cccgccgccc gagctgattt
     4021 ccacctccca gatgtccgtg tacttcctgt tcgtggcggt atccgagtcc tccttgttgc
     4081 ggatcaccgt gggccggagg aacaccagaa gattgctctt ggaacgtgac ttgctgttcg
     4141 atcggaacaa cgcgcccaga ccggggatgt cgccgagcag cggcactttc ttcaccgtgt
     4201 ccgtgatgtc gtcctgaatc agcccgccga gcaccacggt ctgcctgtct tccgccagta
     4261 tcgtggtctc gatgctccgc ttcgacgtga caacgtccga gaacccgtcg ttgccgatgg
     4321 gcgcgttgat cacgttggtg atttcctggg cgacctcgag ccttaccgaa gtgccctcgt
     4381 gcacgtgggg cgtcacggtg agctgcaagc ccacgtcttc acgctggacg gtggtaaagg
     4441 ggttgctgct gtcgtttccg ctggtggtga acgatccggt ccggaacgga acctcgttac
     4501 cgacgacgat cttggcttcc tggttgtcca gggtcaggat gctgggcgtc gacagcaggt
     4561 tcgcgttgga gttggtggcg agcgcgcgaa cgatggcgcc gaaggagacg tctcccctgt
     4621 cgatgcgcgc agcggccagg gtcgggctcg tgatggaggc gagcccgctg accggatcga
     4681 tctctccgtc gtcgccgatg atgttgccga tgaggctgtt gatcgcgccg tcgaggctcg
     4741 tgctggccag gggtacggtc tggccggact cgtccacgat ggccatctcc acgccgatat
     4801 ccgaggcgtt cgtgatggtt acctccacca cggcggcctc gatgagaacc tgggttcgcc
     4861 gcacatccag gctttccaga atttcgagga tctcgttcat ggtgcctgga tcggctcgaa
     4921 ccacgatcgc attcagcgtc tcgtcggcct gaatgttcgg gcgctccgtg gcgccgtctt
     4981 cgccgcggga acggtcctcg atgaccgagt tgagtatctc ggaaaccttt accgcgtcgg
     5041 cgtgcttgag gtagatgacc tgagtcgcgc cggcggtggt tgccggctgg tcgagcttct
     5101 cgatgatctt cagcacctca gcaacgggcc gcggcttgcc tttgagcacc agcgagttgt
     5161 tgcgctcgtt ggcgatgatc tgaatgcgct gggggccctg agcgttgcgg cccatctgat
     5221 ctggcgccac cttttccagc agcgcgacga tcgttccgac ccaggcttcc ttgagcggta
     5281 ccaccacgat ttcctcttcg tcggctacgt cgatctgctc gatcatcctc atcaggcgca
     5341 ggatgttgtc ggcgtggtcg ctgatgatga cgacgttggg cacggcaacg gcggcgatgt
     5401 ggccgtactg ggggatgagc ggccggagga tcttgacgag ctcggcggac tccacgttct
     5461 gggccgacac aacccgggtc acgagctctt cgggcggcac ttcgccgagc tccccgctca
     5521 cgcccggcgt ctgcttcccg gtagcgttct gctggatacg gatcacgtca cccgatggcg
     5581 ttgccgtgaa gttgtgaacg cgcaggacgg agaggaacag cgcgtagacg gcttcctcgt
     5641 ccatgctggt gttggaaatg acggtaacgt tgcctttcag ccggggatcg atgacgaacg
     5701 tcttgccggt aatggcggca acctgggcga cgaactcctg gatgtcggcg ttcttgatgt
     5761 tgatcttcca ggtctgctcc tgtccctgcg cgggcaagct cgtgggcagc agcagaagaa
     5821 ggaccatcgc gtaggttagg gtggtggcga atatcttatt catgcgtttt tcaacgggtc
     5881 ctatggcaag gaggcggtca cgaagaaccg acgcgttccg cgctgtactt cgaggcgagc
     5941 ggatccctgg gccagcatgt tgtccagttc ctgctggtct tggttgatgt ccccgacggg
     6001 tcgaccgttg acggaaagaa tcacgtcccc gggctgcagc cccgtctggc tgagaaatgg
     6061 tgagtcgttc agatcgtcca ggcggtagcc ggaggcgtcg ccttcgttca cggcggagat
     6121 acccaggtcg gcgaggacgc cctggggatc ggcctcgata cgctcccggt actcggccag
     6181 cgcttcgctg gtgggtctct tgcggccccg ctcatcccga tcgtcgtcgg catcctcctg
     6241 gacgtcggtc ggcatgtcga acgggagcgc ctggtcggca agatcctcca gatcatcggg
     6301 ggcggctacg aactggccga tcgatgtcgg aaacttcagc gtctccagga cgccggcccg
     6361 ccgaagcacg atgtgatcgg cgtgcacctc gacgagctct gcgttaccgg gcattttctg
     6421 gccgacaacg tagagcattc ccgggcgccc cttctgggcg acgatcgcgg cagagtcttc
     6481 cgatgcgtct gccacgaaga cgccctgcaa ctccagcggc agcctcgtgg ccacggccat
     6541 ggccgtctgc tgcgtggcgg caggcg
//
LOCUS       DBNHKV010000467         3451 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_64057, whole genome shotgun sequence.
ACCESSION   DBNHKV010000467 DBNHKV010000000
VERSION     DBNHKV010000467.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_64057"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..564
                     /locus_tag="AC16PP_15300"
     CDS             142..564
                     /locus_tag="AC16PP_15300"
                     /inference="COORDINATES: protein motif:HMM:NF019500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HIR6483047.1"
                     /translation="MDYLLATAVSVVLVALCISIHYEMLNLMARVVHRPERHRWIIMV
                     MILGLLFAHVVEIWLFGFGYLLAQEQLALGHITGTGDGLFDYIYYSAMVYTTVGFGDQ
                     VPGGAIRMISSTEALAGLSLITWSASFTFLQMQRLWRD"
     gene            683..2242
                     /locus_tag="AC16PP_15305"
     CDS             683..2242
                     /locus_tag="AC16PP_15305"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF017085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050355 - inorganic triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HIR6483048.1"
                     /translation="MNELEAKYLVAAGRKPRRVLEHVLQTLSWAGYRARLRSERLVVD
                     TYFDTPDWALQRAGWAYRLREDEQKKTRTLAVKAIKTGHYGVFRREEFEQSLDAPLNR
                     PGDPPAGQVADQLWVIVTEEASLKPLFHLRNQRTKYDITSDAYPGGTVELSLDRVTVD
                     EAPGCGYTELELELLEGPEDILNDIVEMLNQQSDIFLARVGKYQRGLLASGRPPSLPS
                     AEPTPSPDKECRWLDLGLYNLRCELDDIRRYEPHAWESIHPEGVHQMRVATRRARAAL
                     RAFGDVLPPKAAKRLTRDIRWLTSSLGPLRDLDVQIAHIDAERERLDARDASAQALYR
                     KHLEKKRRRAHRRLVEALASDEYRKLIADFERLLEASASYHGPNRLMTIREAAHTQMK
                     PQLDRLLARGRNARKRPASRRLHRLRIALKRLRYQLEYLEGSYGKPMGNLTRAMEDVQ
                     DSLGNHQDAMVAVAQLERFRRKGGLKSGSRKSVKRLIAAEQENAARYRKRFRKDWRKF
                     EEAAAKMVKRL"
     gene            complement(2247..3047)
                     /locus_tag="AC16PP_15310"
     CDS             complement(2247..3047)
                     /locus_tag="AC16PP_15310"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR6483049.1"
                     /translation="MNAFRVFFVAALVLGAAACEPGHTPFPDGEIVDLSHTYDETTVF
                     WPTADGFQLEVESAGYTDKGYYYAANNFSTSEHGGTHLDAPIHFAEGAPATDEIPLER
                     LVGPGVVIDVSAACTDDADYQIDVADVTRWERANGQTVDGMIVLFRTGFAERWPDVAR
                     YLGTAERGAEAVAKLHFPGLHPELAAWLVAERDVKAVGLDTASIDYGQSTLYETHQIL
                     FKAGIPAFENVANVSRLPASGFSVVALPMKIGGGSGGPLRIIAIVPPA"
     gene            complement(3090..>3451)
                     /locus_tag="AC16PP_15315"
     CDS             complement(3090..>3451)
                     /locus_tag="AC16PP_15315"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR6483050.1"
                     /translation="RSELDALPSVSQALRQELGDVSYDDYLYTANQANRVMVTTVMRD
                     SPARLAGVEPGDVIFSLAGQRVFEANDLLEVASTGSAGETVSLQVMRDTEVVELHMPL
                     GPLGVQTLPYLGEPGPG"
BASE COUNT          613 a         1179 c         1104 g          555 t
ORIGIN      
        1 cggcttgtaa ggccgtcgct ctcccaactg agctaaacac cctggggaat caagggggcg
       61 tatcttaccc cctgacctat ggctgtcaat cggatccgcc ggcacgtggg aggccttggg
      121 cagtcgcgct acaatccgcc catggactac ctcctggcca ccgcggtttc cgtcgtcctc
      181 gtcgcgttgt gcatctccat ccactacgag atgctgaacc tcatggccag ggtcgtgcac
      241 cggcccgaac gccaccgctg gatcatcatg gtcatgatcc tggggctgct gttcgcccac
      301 gtggtggaga tctggctgtt cggcttcggc tacctgttgg cccaggaaca gctggcgctc
      361 gggcacatca ccggcaccgg cgacgggctg ttcgactaca tctattactc ggccatggtc
      421 tacaccaccg tgggctttgg cgaccaggtg ccgggagggg ccatccgcat gatctccagc
      481 accgaggccc tggccgggct ttcgctgatc acctggtcgg cgtccttcac cttcctgcag
      541 atgcagaggc tctggcgcga ctgagcaccg ggcgatcgcc accgatcggc gccgccggcg
      601 ataccgtcga tcggatcatc gatgacaggg cgcgccgatt cgggtaataa tctgtcaact
      661 tctttgattt atttccccgc ccgtgaacga gctggaagcc aaatatctcg tcgctgcggg
      721 cagaaagccc aggcgcgttc tggagcatgt gctgcagacg ctgagctggg ccggttaccg
      781 ggccagactg cgctcagagc gcctggtcgt cgacacctac ttcgacacgc cggactgggc
      841 cctgcagcgg gccggatggg cctaccgcct gcgcgaggac gagcagaaga agacgcgcac
      901 cctggccgtg aaggccatca agaccgggca ctacggcgtg ttcaggcgcg aagagttcga
      961 gcagtctctc gatgccccct tgaaccgccc cggagacccg cccgccggcc aggtggccga
     1021 ccagctatgg gtgatcgtca ccgaggaagc ttccctgaag cctctgttcc acctgcgcaa
     1081 ccagcgcacg aaatacgaca tcaccagcga cgcctatccg ggcggcacgg tggagctgtc
     1141 cctggaccgg gtcaccgtgg acgaggcccc gggctgcggg tacaccgagc tggagctgga
     1201 gctacttgaa gggcccgagg acatcctgaa cgacatcgtg gagatgctga accagcagtc
     1261 ggacatcttc ctggcccggg tgggcaagta ccagcggggc ctgctcgcca gcggacgccc
     1321 gccctcactg ccgagcgcag agccgacgcc cagcccggac aaggagtgcc gctggctgga
     1381 cctcggcctg tataacctgc gctgtgagct ggacgacatc cggcgctacg agccccacgc
     1441 ctgggaatcc atccacccgg agggcgtcca ccagatgcgg gtggccactc gccgggcccg
     1501 ggccgccctg cgcgccttcg gcgacgtcct gccgcccaag gccgccaagc gcctcacccg
     1561 ggacatccgc tggctcacct ccagcctggg cccgctgcgc gatctggacg tgcagatcgc
     1621 ccacatcgac gcggagaggg aacggctcga cgccagagac gcatcggccc aggcgctcta
     1681 ccggaagcac ctggaaaaga agcgccgacg cgcccaccgg cgcctggtgg aagcgctggc
     1741 cagcgacgag tatcgcaagc tcatcgcaga cttcgaacgg cttctggagg cttccgccag
     1801 ctaccacggc cccaaccggc tgatgaccat ccgcgaggcc gcccacacgc agatgaagcc
     1861 gcagctggac cggctgctgg cccggggacg caacgcccgc aagcgccctg cctcgcggcg
     1921 gctgcaccgg ctgcggatcg ccctgaagcg gctacgatac cagctggagt acctggaggg
     1981 ttcgtacggc aaacccatgg gcaacctcac cagggccatg gaggacgtcc aggattctct
     2041 gggcaaccat caggacgcca tggtggccgt cgctcagctc gagcgatttc gcaggaaggg
     2101 agggttgaag tccggctcgc gcaaatccgt gaagcggctg atcgccgcag agcaggaaaa
     2161 cgccgcgcgt taccgcaagc gcttccgcaa ggactggcgc aaattcgagg aagccgcggc
     2221 gaagatggtc aagcggctgt agcccttcag gcgggtggga cgatggcgat gatccgcaac
     2281 gggccgccgc tgccgccacc gatcttcatg ggcaacgcca ccaccgagaa gccgctggcc
     2341 ggaaggcgtg agacgttggc cacgttctcg aacgccggga tgcccgcctt gaacaggatc
     2401 tggtgggtct cgtaaagggt cgactgaccg tagtcgatgc tggccgtgtc cagacccacc
     2461 gccttcacgt cgcgctcggc cacgagccac gccgccagct ccggatgcag cccgggaaaa
     2521 tgcagcttgg ccaccgcctc cgcgccgcgc tcggcggtgc cgagatagcg cgcgacatcc
     2581 ggccagcgct cggcgaaccc ggtgcggaac agcacgatca tgccgtccac cgtctggccg
     2641 ttagcacgct cccatcgggt gacgtccgcc acgtcgatct ggtagtcggc gtcgtccgtg
     2701 caggcagcgc tcacgtcgat cactaccccc ggccccacca gcctctccag cgggatctcg
     2761 tcggtggcgg gcgcgccctc cgcgaagtgg atgggcgcgt ccaggtgcgt cccgccgtgc
     2821 tcggaggtgg agaagttgtt ggccgcgtag tagtagccct tatccgtgta accggccgac
     2881 tccacctcca gctgaaagcc gtcggccgtg ggccagaaca cggtggtctc gtcgtaggtg
     2941 tgagagagat cgacgatctc gccgtccgga aacggcgtat gcccaggctc gcaggcggct
     3001 gcgccgagca cgagcgcggc aacgaaaaac acccggaatg cattcacctc attccccacc
     3061 ggtttcgatc tttgccggaa ttgtagcagt cagcccggcc cgggttcgcc gagatacggc
     3121 agcgtctgca cgcccagggg gcccagcggc atgtgcagct caaccacctc cgtgtcccgc
     3181 atgacctgca gcgacacggt ttctcctgcg ctgcccgtgc tggcgacctc cagcaggtcg
     3241 tttgcttcga acacccgctg gccagccagg gagaatatga cgtcgccggg ttcaacaccg
     3301 gcaaggcgtg ctggagaatc ccgcatcaca gtggtgacca tgacccgatt ggcctggttg
     3361 gcggtgtaaa ggtagtcgtc gtagctgacg tctccaagct cttgccgcag cgcctggctc
     3421 acgctgggca gcgcatcgag ttcactacgg t
//
LOCUS       DBNHKV010000468         3843 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1696076, whole genome shotgun sequence.
ACCESSION   DBNHKV010000468 DBNHKV010000000
VERSION     DBNHKV010000468.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1696076"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="AC16PP_15320"
     CDS             <1..561
                     /locus_tag="AC16PP_15320"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6483051.1"
                     /translation="SERRLPFILHVGSGVLGIPDSWMNDGRPESESARGGAEIIGSKD
                     LTVIHHSAVRFLSVMVLDGVLERHPELRGGVIEIGAGWVPDMLRRLDHAAAIWSRSEP
                     HLATMSRSPSRQIRDQVRFTPYPFEDVAQLVRESYPELYLFSSDYPHAEGGRDPIGRF
                     DAATATLTDSERAQFFSRNFTDLYRQ"
     gene            complement(539..1453)
                     /locus_tag="AC16PP_15325"
     CDS             complement(539..1453)
                     /locus_tag="AC16PP_15325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR6483052.1"
                     /translation="MESIRRALEDDLSLFAVPVFIVAVLFEALWSRGTGRRLYGVQDS
                     LVSLSMLVASAVVEILPRLLAVALMIQLHEMSPLRDVVVRQWWAWALLFVLDDFSYYW
                     FHRVNHEVRLLWAGHVNHHSSEYLNFATALRQGVGERIHKMVFWLWLPLLGFDVGMIL
                     TMIGLNLIYQFWVHTQAVDRLPAWVELIFNTPSHHRVHHASNVRYLDCNHAGVLIVWD
                     RLFGTFSEETAEDPPVYGLTRNVRSFNPAVVLFHEYAALWRDVRRAPGWRQKLGYLLR
                     APGWSHDGEDRRSRTLRRCEAATAGTGP"
     gene            complement(1518..2360)
                     /locus_tag="AC16PP_15330"
     CDS             complement(1518..2360)
                     /locus_tag="AC16PP_15330"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6483053.1"
                     /translation="MAETRKVFVVLDPTTMEQASLQWGEKIALDNRAHRSQDTHLHVY
                     CCVNFKSVTAVAADTFEHVEAATLERVREWVNRIVTHTRSLGVPVDVEVEWDADWRKA
                     IVGAVRRQKPDVVIKNLTQHARLVRLVRDTSDWQLLRDCQCPVLLIKTGRLYGLKKIL
                     VAVKHTEDEEDQAANRGIMARAQGLADDFGAELHAVTCYDPDRRPDRQRFADSFGLQR
                     NQVHAAMGKPEKVLASVAEEIGADMLIIARVARPESTGLLGDTARRVIDEIATDVLVL
                     PMNG"
     gene            2509..3672
                     /locus_tag="AC16PP_15335"
     CDS             2509..3672
                     /locus_tag="AC16PP_15335"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase family protein"
                     /protein_id="HIR6483054.1"
                     /translation="MPNADPFAPEIPEPVLEDLRRRLTTIRWPHVIGEDDWVYGVPQA
                     WLKDMADYWAHEWNWNSTATAMAAFDHYLADMDGIPVHFLHVPGKGPAPKPLILTHGW
                     PWTFWDYKDVIGPLSDPAAFGGDAADAFDVVVPSLPGFGFSTPLTSAGIDVAEVARLW
                     VRLMQDVCGYERFAAHGGDWGALVTAHLGHAHAQSLIGAHLALPLIPGVDRASVPREA
                     YAEDEQWMLERAAQSGPLIRSHVSVHTTDPQTLAYAMSDSPIGTAAWIWERRRNWSDC
                     DGDVERAFDRDHLCTTAALYWCTGAFTSSLRLYFEHFNKPWPLVHDRKPTVEAPTGFA
                     IFPKDVVQMPRRVAAEHTDLRRWSVMPRGGHFGAAEAPDLIVDEIRSFFRDLS"
BASE COUNT          708 a         1299 c         1162 g          674 t
ORIGIN      
        1 agcgagcgcc gtctgccttt catactgcac gtgggcagcg gcgtgctcgg catccccgat
       61 tcgtggatga acgatggccg gccggaaagc gagtcggccc gcggcggcgc cgagatcatc
      121 gggtcaaagg acctcaccgt gatccatcac agcgccgtcc gttttctgtc cgtcatggtt
      181 ctcgacggcg tcctggaacg gcacccggaa ttgcgcggtg gcgtgatcga gataggcgca
      241 ggctgggtgc ccgacatgct gagaaggctg gaccacgccg ccgccatctg gtcccgatcc
      301 gagccgcacc tggccaccat gtcccgatcg ccgtcccggc agattcgcga tcaggtgcgg
      361 tttacgccct atcctttcga ggacgtcgcc caactcgtcc gtgagtccta tcccgagctg
      421 tacctgttct catcggatta ccctcatgcc gagggcggac gcgaccccat cggccgcttc
      481 gacgcggcca ccgccacgct gaccgattcc gagcgagccc agttcttctc gcgcaacttc
      541 acggacctgt accggcagta gcggcctcgc aacgccgcag cgtccgggaa cgccggtcct
      601 cgccgtcgtg gctccagccg ggagcccgga gaagatagcc gagcttctgg cgccatccag
      661 gcgcgcgccg gacgtcacgc cacaaggccg cgtactcgtg gaacaggacc acggcaggat
      721 tgaaagagcg aacgttccgc gtcagcccgt acacgggcgg atcctccgcc gtctcctcgc
      781 tgaacgtccc gaacagtcga tcccagacga tcagcacacc cgcatggttg cagtccaggt
      841 agcggacgtt gctggcgtga tgaacccggt gatgcgaagg cgtgttgaag atgagctcca
      901 cccaggcggg aagccgatcc acggcttgcg tgtgcaccca gaactgatag atcaggttga
      961 gcccgatcat ggtcaggatc atgccgacgt cgaatccgag cagcggcagc caaagccaga
     1021 agaccatctt gtgaatgcgc tctcccaccc cctgccggag ggcggtggcg aagttcagat
     1081 actcagagga gtgatgattg acgtggcctg cccagagcag acgcacctcg tgattgaccc
     1141 ggtgaaacca gtaataagaa aagtcatcca gaacgaacag cagcgcccac gcccaccact
     1201 ggcgcaccac gacgtcccgc agcggactca tctcgtgcag ctgaatcatc agcgccacgg
     1261 caagaagacg gggcagaatc tccaccacgg cggacgccac cagcatgctc agggatacca
     1321 gagagtcctg caccccgtaa agacggcgcc cggtgccacg ggaccacagt gcttcaaaaa
     1381 gcaccgccac gatgaacacc ggcaccgcga aaaggctcag atcgtcttca agtgctcgcc
     1441 tgatgctctc cacgatatcc ctgcccgaac tcgatgggcc gattatatcg aacggggttc
     1501 gcccccgcgg gtccggatca cccgttcatg gggagcacga gaacgtccgt tgcgatctcg
     1561 tcgatgaccc gacgcgcggt gtcgccgagc agccccgtag actcgggcct tgcgaccctg
     1621 gcgatgatca gcatgtccgc accgatctcc tcggccacgg atgccagcac tttttccggt
     1681 ttgcccatgg ccgcgtgcac ctggttacgc tgcagaccaa agctgtcggc aaaacgctgc
     1741 ctgtcgggtc gccgatccgg gtcgtagcag gtcaccgcgt gcagctccgc tccgaaatcg
     1801 tcggcaagtc cctgtgcccg ggccatgatt ccccgattgg cggcctggtc ctcctcgtct
     1861 tcggtgtgct tgaccgccac tagaatcttt ttgaggccgt agagccgacc ggtcttgatc
     1921 aggagcaccg gacactggca atcgcgcagc agctgccaat ccgacgtgtc gcggaccagg
     1981 cggacgagac gtgcatgctg ggtgaggttc ttgatcacca cgtcgggctt ctgccgccgg
     2041 acggccccca cgatggcctt tcgccaatcc gcgtcccact ccacttccac atcgacgggc
     2101 acgccaagcg accgggtgtg cgtgacgatc ctgttcaccc attcgcgaac ccgctcaagg
     2161 gtggccgctt cgacgtgctc gaaggtgtcg gctgcaaccg cagtcaccga cttgaagttg
     2221 acgcaacagt aaacgtgaag atgggtgtcc tgggaccggt gagcccggtt gtccagagct
     2281 atcttctcgc cccattgaag cgacgcctgc tccatcgtcg tgggatcgag caccacgaac
     2341 acttttctcg tctctgccat cgactgctga ctccgcgttg atctcgattg gtcaagtttc
     2401 accgcatcgg cgcggcgggc atatcggcac ttccccgtag acgccacggt tgtctagatg
     2461 gagcggatct gcgagatttc cccactggcc catcttccgg acgatcgaat gcccaatgcc
     2521 gatccgtttg cgccggagat tcccgagccc gtgctcgagg acctgcgacg gcgcctcacg
     2581 actatccgat ggccccatgt catcggcgag gacgactggg tttacggagt tccccaggcc
     2641 tggctgaagg acatggccga ctactgggcc cacgaatgga actggaactc gaccgctacc
     2701 gccatggcgg ccttcgacca ctacctggcc gacatggacg gcatacccgt tcactttctt
     2761 cacgtcccgg gcaaaggccc ggcgccaaag ccgctgatcc tgactcacgg atggccctgg
     2821 accttctggg actacaagga tgtcatcggc ccgctgagcg acccggccgc gtttggcggc
     2881 gatgcagctg acgccttcga cgtggtggtg ccctctttgc ccggtttcgg attttccacc
     2941 ccgctcacca gtgccggtat cgacgtcgcc gaggtagccc ggctctgggt tcggctcatg
     3001 caggatgtgt gcgggtacga gcgcttcgcc gcccacggcg gtgactgggg cgccctggtg
     3061 acggctcatc tgggccatgc gcacgcgcag agcctgatcg gcgcgcacct ggcgctgccg
     3121 ctgattcccg gcgtcgaccg agcctccgta cccagggagg cctacgcgga ggacgagcaa
     3181 tggatgctcg aacgcgcagc gcaatccggc cctctcattc gcagccacgt gagcgtgcac
     3241 acgacggatc cgcaaaccct ggcgtacgcg atgtccgact cgcccatcgg aacggcggcg
     3301 tggatctggg agcggcgcag aaactggagc gactgcgacg gcgacgtgga gcgcgccttc
     3361 gaccgcgatc acctgtgcac caccgcagcg ctgtattggt gcacgggcgc tttcaccagc
     3421 tcgctgcgac tgtatttcga gcacttcaac aagccctggc cgctggtgca cgacaggaag
     3481 cccaccgtag aagcgcccac gggcttcgcc atatttccca aggacgtggt gcagatgcca
     3541 cggcgggttg ccgccgagca cacggatctc cgccgctgga gcgtcatgcc gcgaggcgga
     3601 cacttcggag cggcagaagc tcccgatctg atcgtcgatg agattcgcag ctttttccgc
     3661 gacttgagct gacaggagcg ccgagatcat ggccaagcac cacgcacaag acctgagccg
     3721 cccctccgac ccgaacctgg cgcccggcga ggcgcgctgc cccgggccga gctttcagga
     3781 catcgtcagg accgaggaca gcgctcggtc cacggctttg accacgcagt cctatgattt
     3841 tct
//
LOCUS       DBNHKV010000469         2908 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_279107, whole genome shotgun sequence.
ACCESSION   DBNHKV010000469 DBNHKV010000000
VERSION     DBNHKV010000469.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_279107"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..260
                     /locus_tag="AC16PP_15340"
                     /pseudo
     CDS             57..260
                     /locus_tag="AC16PP_15340"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
     gene            243..737
                     /locus_tag="AC16PP_15345"
     CDS             243..737
                     /locus_tag="AC16PP_15345"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6483055.1"
                     /translation="MAPIPSCVLMYKQELPLMFPEDADVEQVKKAFFDPFEYLVLRHK
                     AGLIDTGFGKPLGKVAYHVACHQRVQNIGMKTREFLSLIPDTEVTAIERCSGHDGTYA
                     VKSETYEKAMKIARPVVNRVKQAEADTFGSDCPMAGRLIAHGMEDDGAAEHPITMVRR
                     AYGV"
     gene            741..1322
                     /locus_tag="AC16PP_15350"
     CDS             741..1322
                     /locus_tag="AC16PP_15350"
                     /inference="COORDINATES: protein motif:HMM:NF023432.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HIR6483056.1"
                     /translation="MQKLNRTDLWSLEQYSEERPAFRAEVIAHKKARRVAIGPHATLC
                     FEDALTMKYQIQEMLRVEKIFEAAEIEEELTTYNPLIPDGANWKATFLIEYPDVEERH
                     RALATMGGIEDTIWVQIGDGPRIYAIANEDMPRSEAGKAAAVHFLRFELDGASVQAAK
                     EGAPILAGIDHASLKGAVTLDPARAESLRRDLD"
     gene            complement(1350..2234)
                     /gene="oxyR"
                     /locus_tag="AC16PP_15355"
     CDS             complement(1350..2234)
                     /gene="oxyR"
                     /locus_tag="AC16PP_15355"
                     /inference="COORDINATES: protein motif:HMM:NF008361.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HIR6483057.1"
                     /translation="MNLRDLKYLVAVAERRHFGKAADACHVSQPTLSTQIKKLEEELG
                     VVLLERTNRQVMLTPVGERIVAQAQRVLREVDDLVHTAEQFRDPFGGEFRLGLIPTVA
                     PYLLPRILGPIRKSFPNLKIRLTEGQTAVVGERLRAGDLDAVILALPLEEENVVEAEL
                     YREPFYFAASKNHPKAGKREVALSDLDQEQVLLLEDGHCLRDQALEVCNSQNAVENTN
                     FRATSIETLRQMVAADVGITLMPELAIPQKHGAVRYIPFKGNGPHRRIGLCWRGSSSR
                     AALLHDMAGVLREVMAGG"
     gene            2300..>2908
                     /locus_tag="AC16PP_15360"
     CDS             2300..>2908
                     /locus_tag="AC16PP_15360"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR6483058.1"
                     /translation="MTPAIDVLHRSADLLVLNKPANVSLLADRSGEPCYWDALRQALG
                     DRTPYLVHRLDKGTSGVLLVALTRSAQRDLTRAFQQRAVRKFYVAWAVGSMALTSTHT
                     IDLPLQRGRKSRFRVAGPREQIRQQGTRWVLPATDTAGHPAITRIRTLANGHDRSLML
                     LAPRTGRSHQLRVHLSWIGYPIVGDHLYGRPTDTMPRADRLQL"
BASE COUNT          552 a          977 c          908 g          471 t
ORIGIN      
        1 gcgagagaac cacgggcaag gtcgccatct acatcacctg atacggcgac cacaacgagc
       61 cccaggtggt cgaagacctc atcgcggtgc tcaatcacaa cgggattccc gtaaagattc
      121 tcaaggacgc caaatgctgc ggcatgccca agctggagct cggcgacctg agcaaggtcg
      181 cgagcctgaa ggataagaac ctgccgctgt tcctggaggc catcgaggca ggttacgaca
      241 tcatcgcgcc gatcccctcg tgcgtgctga tgtacaaaca ggagctgccg ctcatgttcc
      301 ccgaggacgc cgatgtcgag caggtcaaaa aggccttctt cgaccccttc gagtacctcg
      361 tgctgcgcca caaggccggg ctgatcgata ccgggttcgg caagcccctg ggcaaggtcg
      421 cctaccacgt ggcctgtcac cagcgggtgc agaacatcgg catgaagaca cgggagttcc
      481 tgagcctcat ccccgacacg gaggtcaccg ccatcgagcg ctgctccgga catgacggca
      541 cctacgccgt caaaagtgaa acctacgaaa aggccatgaa gatcgcccgg cccgtggtca
      601 atcgggtcaa gcaggccgaa gccgacactt tcggctctga ctgccccatg gcgggtcggc
      661 tgatcgcgca cggaatggag gatgatgggg ccgccgagca tccgatcacc atggtccgtc
      721 gcgcctacgg agtttgaacc atgcagaaac tgaaccgcac cgatctgtgg agcctggagc
      781 agtattccga ggagcgcccg gcgttccgcg cggaggtgat cgctcacaaa aaggcccgcc
      841 gcgtagccat cggcccccat gccacgctgt gcttcgaaga cgctctcacc atgaagtacc
      901 agatccagga gatgcttcgc gtggagaaga tcttcgaggc cgcggagatc gaggaggagc
      961 tgacaaccta caacccgctg attcccgacg gcgcaaactg gaaggcgact tttctcatcg
     1021 agtacccgga cgtggaggaa cggcaccgtg cgctggccac catgggcggg atcgaggaca
     1081 ccatctgggt gcagatcggc gacgggccgc gcatctacgc gatcgcgaac gaggacatgc
     1141 cgcgttcgga agcgggcaag gcggcggccg tgcattttct ccgcttcgag ctcgacgggg
     1201 cttccgtgca ggcggcgaag gaaggcgcgc ccatcctggc aggcatcgac cacgcgtcgc
     1261 tcaaaggagc ggtgaccctg gacccggcca gagcagagag cctgaggcgg gacctggact
     1321 gaaccggacg gcccgccgcg cgggcaccgt cagccgccgg ccatgacttc ccgaagaacg
     1381 ccagccatgt catgcagcag ggccgcccgg ctggagctgc cccgccagca gaggccgatt
     1441 cggcggtgcg gcccgttgcc tttgaacgga atgtatctca cggcgccgtg cttctgcggt
     1501 atggccagct ccggcatgag cgttatgcca acgtccgccg ccaccatctg gcgcagcgtc
     1561 tcgatgctgg tcgcgcggaa gttcgtgttc tcaacggcgt tctgagaatt gcagacctcg
     1621 agagcctggt cgcgcaggca gtgcccgtcc tcgagcagca gaacctgctc ctgatccaga
     1681 tcgctgagcg ctacttcccg cttgcccgcc ttcggatggt tcttcgaagc ggcaaaatag
     1741 aacggctccc tgtagagctc ggcttccacc acgttctcct cttccagggg caacgcgagt
     1801 atcacggcgt ccaggtcacc ggcacgcagc cgctcgccga ccaccgccgt ctgcccttcg
     1861 gtcagccgga tcttgaggtt ggggaaggac ttcctgatgg ggccgaggat cctgggcagc
     1921 aggtagggcg ccacggttgg aatgaggccc agccggaact ctccaccgaa gggatcccga
     1981 aattgctccg cggtgtggac caggtcgtcg acctcccgca gcacgcgctg ggcctgggcg
     2041 acgatccgct ctcccaccgg cgtgagcatg acctggcggt tggtccgctc gagcaggacg
     2101 acgccgagtt cctcctcgag cttcttgatc tgggtggaca gagtcggctg gctgacgtgg
     2161 caggcgtctg ccgccttgcc gaaatgccgt cgctcggcca cggcaaccag atacttgagg
     2221 tctctcagat tcatggcgcg tatgttactt tgccgcggag gccgcccact acccagcaga
     2281 gccagcccga agccagccaa tgaccccggc aatcgacgta cttcaccgca gcgccgacct
     2341 gctagtcctc aacaagcctg ccaacgtgtc tctgctcgcc gaccgaagcg gagaaccatg
     2401 ctactgggac gcgctgaggc aggcgctcgg cgaccggacg ccctacctgg tccatcgcct
     2461 ggacaaagga acgagcgggg tgctgctggt ggccctgacc cggagcgccc agcgtgacct
     2521 gacccgcgcc tttcagcagc gggccgtgcg caagttctat gttgcctggg cggtcggttc
     2581 catggcgctg acatcgaccc acaccatcga cctgcccttg caacgcggcc ggaaaagccg
     2641 ttttcgggtc gccggcccgc gggagcagat ccggcagcag ggcacgcgct gggtccttcc
     2701 cgcaacggat acggcaggac atcccgccat cacgcggatc cgcacgctcg ccaacggcca
     2761 cgacagatct ctcatgctgc tcgccccccg aaccggtcgc tcccaccagc tgcgcgtgca
     2821 cctgtcctgg atcggctacc ccatcgtcgg cgatcacctc tacgggcggc cgacagatac
     2881 catgccgcgc gccgatcggc tgcagctg
//
LOCUS       DBNHKV010000470        11951 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_207546, whole genome shotgun sequence.
ACCESSION   DBNHKV010000470 DBNHKV010000000
VERSION     DBNHKV010000470.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 11951)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11951)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11951
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_207546"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1042)
                     /locus_tag="AC16PP_15365"
     CDS             complement(62..1042)
                     /locus_tag="AC16PP_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6483059.1"
                     /translation="MQNTEAAVSVEQEKVTTVTDWLSKRIIGQETLLQRLMTALLCGG
                     HLLVEGAPGLAKTRAIKELASAVQGDFHRIQFTPDLLPSDITGTEVYRPEDGSFKFQH
                     GPVFHNLLLADEINRAPAKVQSALLEAMAEHQVSVGRQTFPLPELFLVMATQNPIEQE
                     GTYPLPEAQLDRFLMHVTVSYPSESAETSILHMVRKETGHRDEAATPPFRLTEADIFD
                     ARRAILGLHMAEPVERYIVALIMASREARGELGQWLEFGASPRGTIALDQCARALAWL
                     ERRDYVTPDDVRAIAHDVLRHRLILTFDADAQGVTTDQVIDQLLAEVPVP"
     gene            1289..2566
                     /locus_tag="AC16PP_15370"
     CDS             1289..2566
                     /locus_tag="AC16PP_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007942367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR6483060.1"
                     /translation="MKKLMLGLAVLCVSAPAFADDLSQGNTAWILTSTALVLFMTIPG
                     LSLFYCGLVRTKNVLSVLMQCFVITCFVSILWLLVGYSLAFDADGGNAIIGGLGKVLF
                     AGVSESSMSGDIPETAFALFQLTFAIITPALIVGAFAERMRFSAMLMFSGAWLLLVYA
                     PICHWVWGGGWLGDLGLLDFAGGTVVHITAGVAAVVAALTLGSRRGFPQQAMPPHNMT
                     LTIAGAGMLWVGWFGFNGGSALAANGDAAMAMLVTHLSASAGAFTWMCAEWVRYGKPS
                     ALGAVTGMVAGLGTITPASGFVGPAGALVIGILAGIFCFAATNYLKRSLKIDDSLDVF
                     PVHGVGGILGTLLTAVFASSALGVFSGQEDIVILDQLGVQAIGVVATMAWTGIATFVI
                     LKVTDALVGNRVSDEEEVEGLDLVLHNERGYDL"
     gene            complement(2615..3574)
                     /locus_tag="AC16PP_15375"
     CDS             complement(2615..3574)
                     /locus_tag="AC16PP_15375"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR6483061.1"
                     /translation="MSHFELHVAVLMGGPSAEAEVSRVSAAEVAKGLDQAGHRATLLE
                     LDTGAAQALADLTPDVVFPALHGPPGEDGTVQGFLEILNLPYVGSGVHGSAVAMDKSL
                     AKAVFRRAGLPVADDLVVSVGADLAAAEREILDTLGERVVIKPLNQGSAIGVIRVPNG
                     GDLQAALQTALAFGHRVLVEPYVHGREITVGVLDVTGDEPEPFPVIEIITASDEWYDY
                     TNRYTVGKSEHVVPADVPDPVADALREHAVRAHAELGLRDLSRADYILTDANEIVLLE
                     VNTLPGMTPTSLFPDGARATGRDFPTLVDLLVRRAHQRGADGA"
     gene            complement(3571..5487)
                     /locus_tag="AC16PP_15380"
     CDS             complement(3571..5487)
                     /locus_tag="AC16PP_15380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR6483062.1"
                     /translation="MTSPAYGNWHSPLQAAEIASGAIALRDLRSHGDTLYWLESRPAE
                     QGRTTLMALRPGERPTELTPEPFNVRTRVHEYGGGAYLPCAEGVFFANWSDQRIYLIA
                     NGAISALTRNDQSLRFADFCWDGARERLVAVTEQHGAEGEPVNRLQAIELRNPGELTT
                     LHAGHDFYSSPTVSPDMGHLVFMCWDHPNMPWDGCQLVRSAFAPDGGLADDVVIAGGA
                     QESVQQPQWLGGDALIFLSDRNGYSNLYRYDPSGLYCTLEDGADYGVPQWVFGIRQFA
                     AVSDRHVVAARQGTETELVMVDTEGGFATPLTDAGGAASLGSVVKWQDSVCCLAEFPD
                     RLAAIVRCPLDGSPPTAIHQPGTAPLGAELISRAERVQYPTRDGNRAFAYYYPPRNPE
                     HPVTGAEGELPPLLVMSHGGPTAAASRALNLRIQYYTTRGWAVVDVDYRGSSGYGRAY
                     REALNGRWGVLDVTDCEDAVRHLVDQGLADPDRIAIRGGSAGGFTTLAALTRSSTFKA
                     GASHYGIGDLNALARDTHKFESRYLHTLIGSEAALDERSPISHADALSAPVIFFQGSD
                     DRVVPPAQSRQMAEALRVRGIPVAYVEFAGEGHGFRRAENIARSLENEHQFFCRVFGL
                     IGDDDVKDLEIENL"
     gene            complement(5492..6013)
                     /locus_tag="AC16PP_15385"
     CDS             complement(5492..6013)
                     /locus_tag="AC16PP_15385"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6483063.1"
                     /translation="MAERIYIRPVSRRDRSEFLHMTQRSQHVHEPWISPPLSDATFHS
                     YLKRTEQDDHEGLLVCLTDNDDIVGVININNIVRGSFLSASLGYYVSATQMGHGYMRE
                     GLELVKTYAFCQLGLHRLEANIQPENGRSIKLVRSCGFQLEGLSRSFLFIAGSWRDHE
                     RWTAFHPRATLLP"
     gene            complement(6006..6230)
                     /locus_tag="AC16PP_15390"
     CDS             complement(6006..6230)
                     /locus_tag="AC16PP_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6483064.1"
                     /translation="MRYLSRFALLCMGFALTTAGLMLWHAVGFGFHGIWFFDNDYRLH
                     PLHLLIVGLAMIPPALWEIFVLESRRRSRG"
     gene            complement(6227..7153)
                     /locus_tag="AC16PP_15395"
     CDS             complement(6227..7153)
                     /locus_tag="AC16PP_15395"
                     /inference="COORDINATES: protein motif:HMM:NF024919.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIR6483065.1"
                     /translation="MILTERFADPDTGKRYPLRCGGPIGWRSAGDKHTAIIQLPPIPA
                     GAILVPSLSTAPSPERFQFVLRAASRLWALHEVPCRAAPQPSADTAVTTHVDCFHVHE
                     DLPRAELTVTVTGPSSPARYLLTVTARQLELERAPAPTRSAICKAPPFLSQMALGTSV
                     GPRVCSPACITMLLARHGHAADLKSVSESCFDAVTNLYGIWPLAIRAAAEQGSLGAVE
                     LFDDWEEPLSVLDAGMPFAASVRFGEGELPGAPLTRTAGHLVVVHGADPQDVHVNDPR
                     APEAASVPRRYPAVDFSRAWLRHRGAAYILLP"
     gene            7175..7426
                     /locus_tag="AC16PP_15400"
     CDS             7175..7426
                     /locus_tag="AC16PP_15400"
                     /inference="COORDINATES: protein motif:HMM:NF017574.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR6483066.1"
                     /translation="MNETGLILFSTDHCALCDEALGLLLSMPELAGISLRVVDVADDD
                     DLIATYGQRLPVLSYAGRELDWPFAATDLLRWIRQVEQP"
     gene            complement(7394..8212)
                     /locus_tag="AC16PP_15405"
     CDS             complement(7394..8212)
                     /locus_tag="AC16PP_15405"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR6483067.1"
                     /translation="MSERLTDIGVNLLNRQFQEDRQAVIDRAHRAGVTRMLITCTDLA
                     ESRLGQAFCQSNPEGFWCTAGVHPHHAKDVGPGWLEELAELAGQPCVRAVGETGLDFN
                     RNFSTPEQQLAVFDAQLKLAANTGKPVFVHDRDSDGKTFELLSRYREKLVGVVVHCFT
                     GTQTDLRHYLEAGFYIGITGWVCDPRRGGGLRDIVTMIPLDRLLIETDAPYLRPHNAP
                     VSPEGPARHKRRNEPALLGCVAAQLAELYGIGEADIARASWQNASRLFDLPDPA"
     gene            complement(8209..10104)
                     /locus_tag="AC16PP_15410"
     CDS             complement(8209..10104)
                     /locus_tag="AC16PP_15410"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR6483068.1"
                     /translation="MFFSLLFVAPVAPSFANDDEYRTPYQQRTAYRAALSHLRVGNTQ
                     AFRRIESDLKGYSLHPYLEYHELRARLSSASASEVLAFRERHADLPVTPLLYQRWLER
                     LGQGREWKAFLDHWEESGDPELRCYHSRALYGTGQKEAAFAGVADLWIVGRSQPEACD
                     PLFKVWIDNDNLTEGMVWQRLDLALQANKRQLARYLQRFFSGSRKPWAQSFYNVHVTP
                     DLIARTDRFETDNELSRAVIAHGLRRLAARDAEKASAAWQSYQRSHDFSPETVRELDQ
                     SIAIALAGEGSFPSQRPEKPSSEFVMEMAKAAVAQQNWSEAYYWIERLPSETLGSTRW
                     QYWSARAVGSTHLDSERARLTLQALAERRDYYGFLAAEHLGIETKLNHSALALNPIHT
                     LQLNRMPAVQRALELYAVGDLLNARREWNRVLPDLDQARQVQAAFLIQQSGWTSQAIR
                     MANAASLSDHLDLRFPLAYPEFFKRASHVTAIPESFLIAIARQESAFDPRARSHADAL
                     GLMQLLPSTAKRVARRTGLTAPSDDDLLNPAVNVEIASHHLAELMLRYGNRRPLAAAA
                     YNAGASRVDRWIRDAGGVPMDVWIETIPFGETRNYVKNVLAFAQVYGRLLGTPVPMLQ
                     VNETSIP"
     gene            complement(10187..10651)
                     /locus_tag="AC16PP_15415"
     CDS             complement(10187..10651)
                     /locus_tag="AC16PP_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6483069.1"
                     /translation="MADYRRIMLAVDLTEESNHVAERAIAIAKAFDAELHLVHVIEPL
                     SLAYGGDIPMDLSSVQEQIHDQAKSHLAEFANRLGVPAERQNLIFGRPESEIHRVAES
                     SEADLIVVGSHGRHGLALLLGSTANGVLHGATCDVLAVRVGSIAEKSPDSDS"
     gene            10725..11297
                     /locus_tag="AC16PP_15420"
     CDS             10725..11297
                     /locus_tag="AC16PP_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6483070.1"
                     /translation="MTWLIIGLILLAAFGPVLWLVPSRKDRHLSALREQARLEGLVVE
                     LRRLPKLQPSAHERVTAGGRVKKPVQECAAYMQMLRRRLVMLPGWRVHRGTGDLEARG
                     GWYFDFELRARGDAFDATMSALDPLFGGLPADVVAVELTDRTLLAYWLERGAATRETV
                     SEMALQMRAAEQALLDLDARFKSLAENDDS"
     gene            11362..>11951
                     /locus_tag="AC16PP_15425"
     CDS             11362..>11951
                     /locus_tag="AC16PP_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR6483071.1"
                     /translation="MPPSLVIVESPAKAKTINRYLGSDYIVRSSVGHIRDLPVGGKSV
                     DPKARAREAAKTRKLSPEKKAEYKQERARQQLVRRMGVDPDKGWAAEYEILPGKEKVV
                     DELTKLAKNAEAIYLATDLDREGEAIAWHLRETIGGDPARYRRVTFNEITRRAIQEAF
                     SEPSELDMNQVHAQQARRFLDRVVGFELSPLLWGKIA"
BASE COUNT         2090 a         3720 c         4003 g         2138 t
ORIGIN      
        1 taggcgccgg tcaggatttc ttcccgaccc gagccgagga tcgtgctagc agtcggcgag
       61 ctcagggaac cggcacctcg gcgagcaact ggtcgatgac ctggtccgtc gtgacgccct
      121 gggcgtccgc gtcgaaggtg aggatcaacc gatgccgcag aacgtcgtga gcgatcgcgc
      181 gcacgtcgtc gggtgtgacg tagtctctgc gttccagcca ggccagtgcc cgggcacact
      241 ggtccagcgc gatggtgccc cgtgggcttg cgccgaattc cagccattgg cccagctctc
      301 cgcgcgcttc ccgcgacgcc atgatcagcg caacgatgta gcgctccacg ggttcggcca
      361 tgtgcagacc gagtatcgcg cggcgcgcgt cgaagatgtc ggcttcggtc agccggaagg
      421 gcggtgttgc cgcctcgtcc ctgtgtccgg tttcctttcg gaccatgtgg agaatggacg
      481 tctccgcgga ttccgagggg tagctgaccg tcacgtgcat caggaagcgg tcgagctggg
      541 cctcgggcag cgggtaggtg ccctcctgct cgatggggtt ctgggtcgcc atgacgagaa
      601 aaagttcagg cagggggaac gtctgacgcc caacgctgac ctgatgctcg gccatcgcct
      661 ccagcaacgc cgattgaacc ttcgccgggg cacggttgat ctcgtcggca agcagcagat
      721 tgtggaaaac gggaccgtgc tgaaacttga acgagccgtc ctccgggcgg tacacctcgg
      781 ttccggtgat gtcgctgggc agcagatcgg gcgtgaactg gatgcgatga aaatcgccct
      841 gaacggcgct tgcaagttcc ttgatggcgc gggtcttggc cagccccggc gcgccttcta
      901 ccagcagatg gccgccgcaa aggagcgcgg tcataagacg ctggagcagg gtctcctgcc
      961 cgatgatgcg cttagatagc cagtcggtca ccgtagtgac cttctcctgt tcgaccgaaa
     1021 ctgctgcttc cgtgttctgc atatcgtcct catgcgcgcc cgggcaagcc cgccggggcg
     1081 tccgggccgg gtattgtagc cttccggcaa cgaaatacat cctcgacgcc ggcgttccgg
     1141 tccgcgtcaa tcgagcagca cgcggatcgc ctctggcacc cctggcgaaa tcgaagtaaa
     1201 ttcgttgatg aaatcaagcg gtgtgcggac atccgggaac cggagacaca gaaagccggg
     1261 cgggtggcga cataagaggg gactatcgat gaaaaaactg atgttggggt tggccgtgct
     1321 gtgtgtgtcg gcgcccgcgt tcgctgacga cctgagccag ggcaacacgg cctggatcct
     1381 cacgtcgacg gcgctggttc tgttcatgac cattcctggt ctttccctct tttactgcgg
     1441 gctcgtccgg accaagaacg tgctcagcgt gctcatgcag tgcttcgtca tcacgtgctt
     1501 cgtgtccatc ctctggttgc tggtgggata tagcctggcg ttcgacgccg acggcggcaa
     1561 cgccatcatc ggcgggctgg gcaaggtcct gttcgccggg gtgtctgaga gttccatgtc
     1621 gggagacatc ccggagacgg ccttcgcgct cttccagctt acctttgcaa tcatcacccc
     1681 cgcgctcatc gtgggcgcct tcgccgagcg gatgcgcttc tctgcgatgc tgatgttcag
     1741 cggtgcttgg ctgctgctcg tatacgctcc gatatgtcac tgggtctggg gcggcggctg
     1801 gctcggcgac ctcgggctcc tggattttgc cggcggcacc gtggtccaca tcaccgccgg
     1861 cgttgccgcg gtggtcgcag cgctcacgct cgggtcccgc cgcggtttcc cgcagcaggc
     1921 catgccgccg cacaacatga cgctcaccat tgcgggggcc gggatgctct gggtggggtg
     1981 gttcggattc aacggcgggt ctgccctggc agccaacggc gacgctgcga tggccatgct
     2041 ggtcacccat ctgtccgcgt ccgccggcgc gttcacctgg atgtgcgccg agtgggtcag
     2101 atacggcaag cccagcgcgc tgggcgccgt gacgggtatg gtcgccgggc tcgggaccat
     2161 cacgccggca tcgggcttcg tcggtcccgc cggcgcgctc gtcatcggca ttctcgccgg
     2221 gatattctgc ttcgctgcaa ccaactacct caagcggtca ttgaagatcg acgactcgct
     2281 ggacgtgttc cccgtccacg gggtcggcgg gattctggga acgctgctca cggccgtttt
     2341 cgccagcagc gccctgggcg tcttcagcgg gcaggaggac atcgtgatcc tcgaccagct
     2401 cggcgttcag gccatcggcg tggtcgccac catggcgtgg acgggcatag cgacgttcgt
     2461 gattctcaag gtgaccgacg cgctggtcgg gaaccgcgtc tccgacgagg aagaggtgga
     2521 agggctcgat ctcgtccttc acaacgaacg tggatatgac ctgtaacacc ctgaaaacta
     2581 tctaagtttt cagggtcatt tcccaagggg gcggtcaggc tccgtctgcg ccccgctggt
     2641 gggccctgcg gaccaggagg tcgaccagcg tggggaaatc ccttccggtg gcccgggcgc
     2701 cgtccgggaa aaggctggtc ggcgtcatcc caggcagcgt gttcacctcc aggaggacga
     2761 tctcgttggc gtccgtgagg atgtaatcgg ctctcgacag gtccctcaag ccaagctccg
     2821 cgtgggcgcg aacggcatgc tctctcagag cgtcggccac cgggtccggc acgtcggctg
     2881 gaaccacgtg ctcgctcttt cccacggtgt agcggttggt gtagtcgtac cactcgtcgc
     2941 tggcggtgat gatctcgatc acgggaaacg gctcgggctc gtccccggtg acgtccagca
     3001 cgccgaccgt gatctcgcgg ccatggacgt agggctcgac gaggacccta tgcccgaacg
     3061 ccagggccgt ttgcagcgcg gcttgcagat cgccgccgtt cggcacgcgg atcacgccga
     3121 tggcggagcc ctgattcaga ggctttatca ctacgcgctc cccgagcgtg tccagaatct
     3181 cccgctcggc ggccgccaga tcggccccga cgctcacgac gaggtcgtcg gccaccggca
     3241 ggcccgcacg gcgaaacacg gccttggcca ggcttttgtc catggccacg gcgctgccgt
     3301 gaacgccgct gccgacgtag ggcagattga gaatttccag gaagccctga acggtgccat
     3361 cttcccccgg cgggccgtga agagcgggaa acaccacgtc cggagtcagg tcggcgaggg
     3421 cctgcgccgc tccggtatcc agttcaagaa gggttgcccg gtgaccggcc tgatccaagc
     3481 cctttgcgac ctccgcggcg gataccctgg aaacttcggc ttcggcagat ggtccgccca
     3541 tgagaacggc aacgtgcagt tcgaaatggc tcacaggttc tctatctcca aatcctttac
     3601 gtcgtcatca ccaatgagac cgaatacgcg gcagaagaac tggtgctcgt tctcgagcga
     3661 cctggcgatg ttttcggccc gtcggaagcc gtggccttcg ccggcaaact cgacgtaggc
     3721 caccggaatg ccccgcacgc gcagcgcctc ggccatttgc ctggactgtg cgggcgggac
     3781 cacccggtcg tcgctgccct gaaagaagat gaccggagcc gacagcgcat ccgcatggga
     3841 gatgggcgag cgttcatcca gagctgcctc gctgccgatg agggtgtgca ggtagcgtga
     3901 ttcgaacttg tgcgtatccc tggccagcgc gttaagatcg ccgatcccgt aatggctggc
     3961 gccggccttg aaagtgctcg agcgagtcag cgccgccagc gtggtgaacc ccccagcgct
     4021 gcctccgcga atggcgatcc ggtccgggtc tgcgagcccc tggtcgacca ggtggcggac
     4081 cgcgtcttcg cagtcggtga cgtccagcac gccccagcga ccgttgagcg cttctcggta
     4141 ggctcgcccg tatccggagc tgccgcggta gtccacgtcg accaccgccc agccgcgggt
     4201 tgtgtagtac tgaattctca ggttcaacgc tcgggacgcc gctgccgtgg ggccaccgtg
     4261 gctcatgacg agaagcggcg gaagttcccc ctcggcgccc gtaaccgggt gttcgggatt
     4321 tcgaggtgga tagtaatagg cgaaggccct gttgccgtcg cgggtcgggt actgaacccg
     4381 ctcggcgcgg gagatcagct cggcgcccaa cggcgccgtg cccggctggt gaatggccgt
     4441 cgggggcgag ccgtcgagcg ggcagcgcac gatcgcggca agccggtcgg gaaactcggc
     4501 gaggcagcag acgctgtctt gccatttcac caccgagccc aggctcgcag caccgccggc
     4561 gtccgtcaac ggcgtggcga acccgccctc ggtgtcgacc atgacgagtt ccgtctcggt
     4621 gccctggcgg gcggcgacga catgccggtc gctcaccgcg gcgaactgac gaatgccgaa
     4681 cacccactgg ggcacgccgt agtccgcacc gtcctccagc gtgcagtaaa gcccgctggg
     4741 gtcgtatcga tagagattcg agtagccgtt gcggtcgctc aagaagatca gcgcatcgcc
     4801 accaagccac tgaggctgct gcaccgactc ctgggcaccg ccggcgatga cgacgtcgtc
     4861 ggcgaggccg ccatcgggag cgaacgccga tcgcaccagc tggcagccgt cccagggcat
     4921 gttggggtga tcccagcaca tgaagacgag atgtcccatg tctggagaaa ccgtcggcga
     4981 tgagtagaag tcgtggccgg cgtgcagcgt ggtgagctcg ccggggtttc gcagctcaat
     5041 tgcctggagt ctgttcacgg gctcgccctc ggccccgtgc tgctcggtca cggcaaccag
     5101 gcgctctctg gctccgtccc agcagaagtc ggcaaagcgc agggattggt cgtttcgagt
     5161 gagcgcgctg atcgctccgt tggcgatgag gtaaatgcgt tgatccgacc agttggcaaa
     5221 gaacacgccc tccgcgcagg gcaggtaggc gccgccaccg tactcgtgca cgcgcgtgcg
     5281 aacattgaac ggctcggggg tgagctcggt gggccgttct cccggccgaa gggccatgag
     5341 cgtggtgcgc ccctgctctg ccggccggct ctcgagccag tacagggtat ccccgtgaga
     5401 gcgcagatcc ctcagagcga tggctccgga agcgatctcg gctgcctgca aaggggagtg
     5461 ccagttgccg taggcgggag acgtcatggg ctcaaggaag cagcgtggcg cgcggatgaa
     5521 aggcggtcca gcgctcgtgg tcgcgccatg acccggcaat gaacagaaac gacctggaca
     5581 ggccctcgag ctggaaaccg cagctgcgga ccagcttgat ggagcggccg ttctcgggct
     5641 ggatgttcgc ttccagacga tgcaggccca gttggcagaa ggcgtaggtt ttgacgagtt
     5701 ccaggccctc gcgcatgtag ccgtgaccca tttgcgttgc gctgacgtag tagcccaggg
     5761 aagcgctcag aaacgaacct cggacgatgt tgttgatgtt gatcaccccc acgatgtcat
     5821 cgttgtccgt caggcagacc aacagcccct cgtgatcgtc ctgctcggtg cgcttgaggt
     5881 agctgtgaaa cgttgcgtcg ctcaggggtg gagatatcca cggctcgtga acgtgctggc
     5941 tgcgctgcgt catatgcaga aactcgctgc ggtcccggcg cgagaccggt cggatgtaga
     6001 tgcgttcagc cacggcttct tcgtcggctc tccagcacga agatctccca cagcgcgggc
     6061 gggatcatgg cgagaccaac gatgagcagg tgcagcggat gcaatcggta gtcgttgtcg
     6121 aaaaaccaga tgccatgaaa gccgaagcca acggcgtgcc agagcatgag tccagccgtc
     6181 gtcagtgcga agcccatgca cagcagcgcg aagcgcgaaa gatatctcat ggcagcaaaa
     6241 tatacgctgc tccccggtgc ctaagccagg ctctggagaa atcgacggca gggtagcggc
     6301 gcggtacgga agccgcttcc ggcgcccgcg gatcgttgac gtgaacgtcc tgcggatcgg
     6361 cgccgtgcac gaccacgaga tgaccggcgg tgcgggtcag aggcgctccg ggcagctcgc
     6421 cctcgccgaa gcggacgctc gctgcgaatg gcattccagc atccagcacg ctcagcggct
     6481 cctcccagtc gtcgaacaac tccaccgcac caagggatcc ttgctccgca gcggcgcgga
     6541 tggccagggg ccagattccg taaaggttgg tcaccgcgtc gaagcaggat tcgctcacgg
     6601 acttcagatc tgcagcgtgt ccgtgccgcg cgaggagcat ggtgatgcag gcgggcgagc
     6661 acacgcgtgg cccgacagac gtccccagtg ccatctgaga gagaaaggga ggtgccttgc
     6721 agatcgccga tcgcgttgga gcaggcgcac gttcgagctc aagctggcgc gccgtgacgg
     6781 tcaagagata gcgagccggg ctcgaagggc cggtgacggt gaccgtcagc tcggcgcgcg
     6841 gcaggtcctc gtgaacgtga aaacagtcca cgtgcgtcgt gacggcggtg tcggccgacg
     6901 gctgcggcgc cgcgcggcag ggaacctcgt gcagcgccca cagccgggaa gcagcgcgaa
     6961 gcacgaactg gaaccgctcc ggggacggcg ccgtcgagag gctgggtacg agaattgcgc
     7021 cggccggtat gggcggcagc tggatgatcg cggtatgttt gtcaccggcc gaccgccatc
     7081 cgatgggccc cccgcagcga agagggtacc gcttgccggt gtcagggtcg gcgaagcgtt
     7141 ctgtcagtat catgagttcc agcgattgac caacatgaac gaaaccggcc tgattctctt
     7201 ctccacagac cactgcgcgc tatgcgacga ggcgctcggg ctgctgttaa gtatgccaga
     7261 actggccggg atctccctgc gcgtcgtcga tgtggccgat gacgacgacc tcatcgccac
     7321 ctatgggcag cggctgccgg tgctgagcta cgccgggcgc gagctggact ggccattcgc
     7381 cgcgaccgac ctgttacgct ggatccggca ggtcgaacag ccgtgatgcg ttctgccagc
     7441 tggcccgggc gatgtccgct tcgccaatcc cgtagagctc ggcgagctgc gcggccacac
     7501 agccgagcag agcgggctcg ttgcgcctct tgtggcgcgc cggcccttca ggagaaacgg
     7561 gcgcgttgtg cggacgcaga taaggcgcgt cggtttcgat cagcaggcgg tcgaggggga
     7621 tcatggtgac gatgtcgcga aggccgcccc cccgccgcgg gtcgcaaacc catccggtga
     7681 tgccgatgta gaagccggcc tccaggtagt gcctgagatc ggtttgggtg ccggtgaagc
     7741 aatggacgac cacccccacc agcttctccc ggtatcggct gagaagctcg aacgttttgc
     7801 cgtcggagtc tcgatcgtgg acgaacacgg gcttccccgt gtttgccgcc agcttgagtt
     7861 gcgcgtcgaa tacggccagc tgctgctccg gcgtggagaa gttgcggttg aagtccaggc
     7921 cggtttctcc taccgcccgc acgcagggtt gccccgcaag ctcggcgagc tcctccagcc
     7981 agcccggtcc cacgtctttg gcgtgatgcg gatggacgcc ggccgtgcac cagaaaccct
     8041 cggggttcga ctggcagaat gcctggccga gccgggattc ggcgaggtcg gtgcaggtga
     8101 tcaacatccg ggtcactccc gcccggtgag ccctgtcgat cacggcctgg cggtcctcct
     8161 ggaactgacg gttgagcaga ttgacgccga tgtcggtcag gcgctcgctc atgggatgct
     8221 ggtctcgttg acctggagca tgggaacggg cgtgcccaga aggcgcccgt agacctgagc
     8281 gaaggccagg acgttcttga cgtagttgcg ggtttcacca aaggggatgg tctcgatcca
     8341 gacgtccatg ggaacaccgc cggcgtctct gatccagcgg tcgacgcgac tcgcgccggc
     8401 attgtaggcg gccgcggcga gtggtcgccg gttgccgtat cggagcatca actcggccag
     8461 atgatggctc gcgatctcca cgttcaccgc agggttgagc aggtcgtcgt ccgacggggc
     8521 ggtcagcccg gttcgccgcg ccaccctctt tgccgtgctg ggcagcagct gcatgagccc
     8581 caacgcgtcg gcgtgagatc gggcccgcgg atcgaaggcg ctttcctggc gagcgattgc
     8641 gatcagaaag gattcgggaa tggcggtgac gtggcttgcg cgcttgaaaa actcgggata
     8701 ggccagcggg aagcgcagat ccagatggtc cgacaggctt gccgcgttgg ccatgcggat
     8761 ggcctgtgag gtccagccgg attgctgaat gagaaaggct gcctgcacct ggcgtgcctg
     8821 atccagatcg ggcagcacgc ggttccactc gcgccgggcg ttgaggagat cgccgacggc
     8881 atacagctcc agggctcttt gcaccgctgg catgcgattc agctgcaggg tatggatggg
     8941 gttcagtgcc agcgccgagt ggttcagctt cgtctcgata ccgaggtgct ccgcggcgag
     9001 aaatccgtag taatcgcgtc tttctgccaa ggcctgcagg gtcagcctgg cgcgctccga
     9061 atcaaggtga gtggacccga cggcccgtgc ggaccagtat tgccagcggg tgcttcccag
     9121 ggtttccgac ggcagacgtt cgatccagta gtaggcctcg gaccagttct gctgggccac
     9181 cgccgccttg gccatttcca tcacgaattc tgacgatggc ttctctggcc gttgcgatgg
     9241 aaaagagcct tcgccggcaa gcgctatcgc aatcgattgg tccagctccc ggacggtctc
     9301 cggggaaaag tcgtgggacc gttggtagct ctgccacgct gcgctggcct tctccgcgtc
     9361 tcgggcggcc aggcgccgca atccgtgggc tatcacggct ctggaaagct cgttgtccgt
     9421 ctcgaagcgg tcagtccgcg cgatgaggtc tggcgtgacg tgcacgttgt agaacgattg
     9481 agcccacggc tttcgtgaac cggagaagaa gcgctgaaga taccgggcga gctgacgttt
     9541 gttggcctgt agcgccagat ccaggcgctg ccagaccatg ccctccgtca agttgtcgtt
     9601 gtcgatccac accttgaaca acggatcgca cgcttccggc tgggacctgc ctacgatcca
     9661 caggtccgca acgcctgcga aagcagcctc cttctggccg gtgccgtaga gagcccggct
     9721 gtgatagcaa cgcagctccg gatcgccgct ttcctcccag tggtcgagaa acgccttcca
     9781 ctctcgcccc tggccgagtc gctccagcca gcgctggtag agaagcgggg tgaccggaag
     9841 gtcggcgtgc cgctcgcgaa atgccagcac ctcgctcgcc gaagcgctgg acaggcgagc
     9901 gcggagctcg tggtactcca gatacgggtg tagcgagtaa cccttcaggt ccgactcgat
     9961 gcggcgaaac gcctgcgtat tgccgacccg caggtgcgaa agagcggccc gatacgcggt
    10021 gcgctgctgg tatggggtgc ggtactcgtc atcgttggcg aacgacggcg ccaccggcgc
    10081 cacgaacaac agcgagaaga acatcaggtg ccagcgccga cagtggccgg gcagagcggt
    10141 gctgggtgac gggagggtca gccggcccct tagactggca atcagatcag ctatcggaat
    10201 ccggcgactt ctccgcgatg ctgcccacgc gaacggccag aacgtcgcag gtggcgccgt
    10261 gcagcacgcc gttggcggtg gagccgagca gcagggcaag gccgtgccgg ccgtggcttc
    10321 ccaccacgat gaggtcggct tcgctggatt cggcgacgcg atggatctca ctctcggggc
    10381 gtccgaagat gaggttctgg cgctctgccg gaacgcccag acggttggcg aactcggcga
    10441 gatgggattt agcctgatcg tggatctgct cctgaacaga cgacaggtcc atgggaatgt
    10501 cgccaccgta ggcaaggctc aatggttcga tgacatgcac gagatgaagt tccgcgtcga
    10561 aggccttcgc aatagcgata gcccgctccg cgacgtgatt ggactcctcg gtgagatcca
    10621 ctgccaacat gattcgacgg tagtcagcca tgttgcctcc cggcacgaat tgcggcgacg
    10681 atacacacaa tccaaaggcc cctcaacctc tcggggagcg tcccatgacg tggttaatca
    10741 tcggtttgat cctgctagcc gccttcggtc cggtgctctg gctggtgccc agcagaaaag
    10801 accgccacct cagcgcgttg cgagagcagg cccgactcga ggggctcgtg gtggaattga
    10861 ggcgattgcc caagctgcag ccgtccgccc atgagcgggt cacggccggg ggacgcgtca
    10921 agaaaccggt gcaggagtgc gcggcgtaca tgcagatgct gcggcgaaga ctcgtgatgc
    10981 tgcccggctg gcgcgtccat cgtggcaccg gggatttgga agcccgcggc ggctggtatt
    11041 tcgacttcga gctccgggca cggggggacg cattcgacgc gacgatgtcc gctctggacc
    11101 ctcttttcgg ggggctcccc gctgacgtcg tcgcggtgga gttgaccgac cgaacgctgc
    11161 tcgcctactg gttggaacga ggcgctgcca cccgggaaac cgtgtccgag atggccttgc
    11221 agatgcgagc agcggagcaa gcgctgctcg atcttgatgc ccgttttaag agcctggctg
    11281 agaacgacga ttcttgacag ccgttttggc caggccgtat aacggcgccc gaacccatcc
    11341 tcgggcgctg ttcaggcaca tatgccccct tctttagtca tcgttgaatc accggccaag
    11401 gccaagacga tcaatcgtta ccttggcagc gactacatcg tgcgctccag cgtgggtcac
    11461 attcgggatc tgccggtggg cggcaagagc gtcgatccca aggcgcgagc cagggaggct
    11521 gccaagacgc gcaagctgtc tccggagaaa aaagcggagt acaaacagga gcgggcccgc
    11581 cagcaactgg tcagacgcat gggcgtagac ccggacaagg gctgggcggc cgaatacgag
    11641 atcctgcccg gaaaggaaaa ggtcgttgac gagctcacca agctcgccaa gaacgccgag
    11701 gccatctacc tcgccaccga cctggatcgc gaaggggagg ccatcgcctg gcatctcaga
    11761 gagaccatcg gcggcgatcc ggctcgttat cggcgggtga cgttcaacga aataacccgg
    11821 cgggccattc aggaggcctt ctccgagccg tcagagctcg acatgaacca ggtgcacgct
    11881 cagcaggcca ggcgattcct ggaccgtgtc gtcggcttcg agctctcgcc gctgctgtgg
    11941 gggaagattg c
//
LOCUS       DBNHKV010000471         2549 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1342512, whole genome shotgun sequence.
ACCESSION   DBNHKV010000471 DBNHKV010000000
VERSION     DBNHKV010000471.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1342512"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /gene="pgk"
                     /locus_tag="AC16PP_15430"
     CDS             complement(<1..359)
                     /gene="pgk"
                     /locus_tag="AC16PP_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR6483072.1"
                     /translation="MSQRRNMTDLDLAERRVLIREDLNVPIRDGRITSDARVRAALPT
                     IEAAMRAGARTLVMSHLGRPVEGEFDPALSLAPVAGRLGELLGVDVPLVGLDDVGVID
                     PGAVSLLENVRFLKGEGA"
     gene            complement(367..2349)
                     /gene="tkt"
                     /locus_tag="AC16PP_15435"
     CDS             complement(367..2349)
                     /gene="tkt"
                     /locus_tag="AC16PP_15435"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR6483073.1"
                     /translation="MVTRTDRANAIRALAMDAVQAANSGHPGAPMGLADIAEVLWRDF
                     LKHNPADPAWANRDRFVLSNGHGSMLLYSVLHLSGYAVTMDDLRSFRQLHSRTPGHPE
                     YSECPGVETTTGPLGQGLANAVGMALAEERLSRRFNRDGFSVVDHRTWVIVGDGCLME
                     GISHEAASLAGTLQLGKLICIYDDNGISIDGDVKAWFTEDVPARFEAYGWRVIRDVDG
                     HDPNSIQRALDDAVSRQDRPTLVCCRTVIGFGAPNKQGTADVHGSALGEEEVANARRE
                     LGWAAPPFEIPEPVYASWDCREKGAEQQRSWEALLAEYRAAHPEDAAELERRVSGDLP
                     EGWEGPIRALAEASQEAMEPLETRKSSQRCLAALAGALPELFGGSADLTGSNGTRWDG
                     ADEDRYLSFGVREFGMTAITNGMALHGGFRPFSGTFLVFMEYARNAVRLAALMKIPNI
                     FVYTHDSVAVGEDGPTHQPIEQLTNLRTTPGLSTWRPCDTVESAVAWEAAVASRQRPT
                     ALIFTRQKTAPQPRSAQAFADIRRGGYVLKAEAGTLAAVVIATGSEVEIAVAAAASLE
                     SDGVGVRVVSMPSTDVFLSQDRAYQDAVLPPALRARVAVEAAHPDYWYRFVGLDGAVV
                     GIDRFGLSAPGGQAMAALGITADNVAEAVRSVLKEA"
BASE COUNT          446 a          895 c          814 g          394 t
ORIGIN      
        1 gcaccctcgc cctttagaaa gcgaacgttc tccagcagcg agacggcgcc ggggtcgatc
       61 acgcccacgt cgtccaggcc gaccaatggg acatccacgc ccagcaactc gcccagccgc
      121 cccgccacgg gggccaagga caacgccggg tcgaactcgc cctccaccgg gcggccgagg
      181 tgagacatca ccagggttct cgcgccggcg cgcatggcgg cctcgatggt cggcagcgcc
      241 gcccggaccc gcgcgtcgct cgtgatacgg ccatcgcgga tcggcacgtt cagatcctcc
      301 cggatcagca cccgccgctc cgcgagatcc agatccgtca tgttccttcg ctgcgacatg
      361 aaaagatcag gcctccttca gaacgctccg gacggcctcg gcgacgttgt ccgcggtgat
      421 gccgagggcg gccatggcct ggccacccgg cgcggagaga ccgaagcggt cgatgcccac
      481 gacggcgccg tccaggccca cgaatcgata ccagtagtca ggatgcgcgg cctccaccgc
      541 cacccgcgct cgcagcgcag ggggcaatac ggcgtcctgg taggcacggt cctgggacaa
      601 gaaaacgtcg gtggagggca tggagaccac ccgcaccccg acgccgtccg actcgaggct
      661 cgctgccgcc gccacggcga tctccacctc ggaccccgtg gcgatgacca cggcggccag
      721 cgtgccggcc tcggctttca ggacataacc tccccggcgg atgtcggcga acgcctgagc
      781 agaacggggc tggggcgccg tcttctggcg ggtgaatatc agcgcggtcg gtcgctgccg
      841 ggacgccacc gccgcttccc aggcaaccgc actctccacg gtgtcgcaag ggcgccacgt
      901 cgacaatccg ggcgtggtcc gaagattggt gagctgctcg atgggctgat gggtcgggcc
      961 gtcctcaccg acggccacgg agtcatgggt gtacacgaag atgttgggga tcttcatcag
     1021 cgccgcaagc cgcacggcgt tgcgggcata ctccatgaac accagaaaag ttccggagaa
     1081 cggtcgaaag cccccgtgga gggccatgcc gttggtgatg gccgtcatgc cgaactcgcg
     1141 aacgccgaag ctcaaatacc ggtcttcgtc tgcaccatcc cagcgggtcc cgttggagcc
     1201 cgtgagatcc gcggagcctc cgaaaagctc cggcagggcg ccggccagcg cggcaaggca
     1261 tcgctgggac gatttccgcg tctccagggg ctccatcgcc tcctgggacg cttcggccag
     1321 ggcgcggatc ggaccctccc aaccttccgg cagatcgccg gacacgcggc gttcgagctc
     1381 cgccgcatcc tccgggtgcg cggcgcgata ctccgccagc agcgcctccc aggaccgctg
     1441 ctgctcggcg cctttctccc gacagtccca ggaggcgtag acgggctccg gaatctcgaa
     1501 gggcggcgca gcccagccga gctcccggcg ggcgtttgcc acctcttctt cgcccagcgc
     1561 cgagccgtgc acgtcggcag ttccctgctt gttgggcgcg ccaaaaccga tcaccgtcct
     1621 gcagcaaacc agggttggtc gatcctgccg gctcaccgcg tcgtccagag cccgctgaat
     1681 cgaattcggg tcgtgcccgt ccacgtcgcg tatgacgcgc caaccatagg cctcaaagcg
     1741 agccgggacg tcctccgtga accaggcttt gacgtcccca tcgatggaga tgccgttgtc
     1801 gtcgtagatg cagatgagct tgccgagctg cagggtgccg gcaagcgaag ccgcctcgtg
     1861 agagatcccc tccatgaggc agccgtcacc gacaatcacc caggtccggt gatctaccac
     1921 cgagaaaccg tcgcggttga agcgccggga gagccgctct tcggccagcg ccattcccac
     1981 tgcgttagcc aggccctggc ccagcggacc ggtggtcgtt tcgacacccg gacattcgct
     2041 gtactccggg tgaccgggcg tgcgcgagtg aagctgcctg aacgagcgca ggtcgtccat
     2101 ggtgaccgca taccccgaca gatgcagcac gctgtacagc agcatggatc cgtgaccgtt
     2161 ggaaaggacg aaccgatcgc ggttggccca ggccggatcc gccgggttgt gcttgagaaa
     2221 gtcgcgccac agcacttctg caatgtcggc cagacccatg ggcgccccgg gatgaccgga
     2281 gttagcggcc tgcaccgcgt ccatggcgag cgcacgaatg gcgttcgccc tgtcagtcct
     2341 cgttaccatt tggttttcgc tagcgatgta agagtaagga tcggtgccag ccccctgccg
     2401 accctgtcta ttgtccgtta ctcgcaccga catgcgcaaa tgccgcgcgc cccatcgggc
     2461 actggccgcc tgggctcgga aaggccgcgc cgtgagtcga accagagaaa agctgtacaa
     2521 tgcgccgctt gccgcaacag ggcctggca
//
LOCUS       DBNHKV010000472         3734 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1130151, whole genome shotgun sequence.
ACCESSION   DBNHKV010000472 DBNHKV010000000
VERSION     DBNHKV010000472.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1130151"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3485)
                     /locus_tag="AC16PP_15440"
     CDS             complement(<1..3485)
                     /locus_tag="AC16PP_15440"
                     /inference="COORDINATES: protein motif:HMM:NF015228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HIR6483074.1"
                     /translation="MDKQLVEGDRLYLIAESLAQDFSLFPTAESQERLPGLLSPRDIL
                     RQKQRLANLDVDAYIEAVVARAEDPGRTAAPVVIRQLGRVVEEVSDGPFYAATVEMDF
                     DGEPRLVGFIAQDRSSRNGVWMPAHHLAAANRIQNYAKRSQPIVTMMDTPGADAEEQA
                     NRENQAHSISRLIAEMSNVDVPNVGIIYGLGYSGGAIPLAASNMILSVRDGVFSTIQP
                     KSLANIARRLNLSWQECAKYVGLSPYELYAQGNIDGVIDYVPDDDVANLRLAIVSGVQ
                     QVEEKVKEFVAENPYILDHYRQSLQRYLNPSEQLKVMQASASMKLTKNPTEYLNVFGV
                     AYRYLRYLKVRQRIKATSTHQYGRLAVRELPQGELTQRAELERRMLFLKWLQDPDRVV
                     YDDALSRAWKNYSDKKQAVHDERGRLAQFLFGEPKKNYEDARALLISSVGVFLYNRWK
                     SESLGNLRALKEMLTRHEDIRHILTVSDLPDARGLIKALRSDYALLPRLREGFTHEGK
                     KLIGEERLEDKSDLYLSSQLTAELNLVITGPLVAPEAGSPRSSDMARNRAYLVERYPG
                     LVMATAKEGAPVPFSDMTVLDVLVSDDLRRDFVTECENLLLFDSVYDQIIANLDSIAE
                     EAQSRQALAKTSLAQLIDTTLGLAATGVTLGGRGSGEPAETSETAKLRRQFIDWYLRI
                     ARMPKGADFFRAVEEWKKAVFPHLSDTLFVVVTHLFDSLLLSFVQSEREGKAYNGRIA
                     PKNIGRRKDFWNRLNIAYRDLLIQNVLRDAKNRRTTGYKAFVDTFFSAFDERDADLLS
                     SDPCEFPGFRLSIEEALNRGIPPCGVVTGIGNFKTGDGDVRVGTVISNVDFQAGSFDM
                     ASAEKFCRLLVECAEQHLPVVCFISSGGMQTKEGAGSLFSMAAVNDRITRFVRDHDLP
                     VIVFGFGDCTGGAQASFVTHPLVQTYYLSGTSMPFAGQIVVPSNLPLNSILSNYLSVV
                     AGAMQGLVQHPFLPELDKELRHIDPEIPVPQESVIDVVARVLSGVFSQQRPVVVAHRP
                     KYTEVDLFRPVRRVLIHARGCTAAKLIRIAQRNGIEVVLVQSDPDMESAAVDMLGEKD
                     SVVCIGGNTPDESYLNAMSVLSVAEHEGTDALHPGIGFLSENSQFAELVRSHGINFIG
                     PPVSSMETMGNKSNA"
     gene            3622..>3734
                     /locus_tag="AC16PP_15445"
     CDS             3622..>3734
                     /locus_tag="AC16PP_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017519304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6483075.1"
                     /translation="MPDLTSLNCAVLTVSDTRTLEDDRSGDLLIELLTADG"
BASE COUNT          693 a         1158 c         1193 g          690 t
ORIGIN      
        1 atggcattgg acttgttgcc catggtctcc atgctggaca ccggaggccc gatgaagttg
       61 atcccgtggg aacgcaccag ctcggcgaac tgggagttct cggaaagaaa accgatgccc
      121 ggatgcagcg cgtcggtgcc ctcatgctcg gccacgctca ggacgctcat ggcgttcagg
      181 tagctctcgt cgggggtgtt gccaccaatg cagaccaccg agtccttttc gcccagcatg
      241 tccaccgcgg cggattccat gtccgggtcc gactgcacca gcacgacctc gatgccgtta
      301 cgctgggcaa tacggatcag cttggcggcc gtgcacccgc gggcatgaat gagcacacgg
      361 cgcacgggcc ggaaaagatc gacctccgtg tacttgggcc ggtgcgccac caccaccgga
      421 cgctgctggc tgaacacgcc cgagagcacg cgcgcgacca cgtcgatgac ggactcctgg
      481 ggaacgggta tctcaggatc gatgtgccgc agctccttat cgagctccgg aagaaacggg
      541 tgctggacca gcccctgcat ggcgccggcg accacggaaa gatagttgga gaggatcgaa
      601 ttgagcggca ggttcgacgg caccacgatc tggccggcga acggcatgct ggtccctgag
      661 aggtagtagg tctgcaccag gggatgcgtg acgaaactcg cctgggctcc ccccgtgcag
      721 tcgccgaacc cgaacacgat gaccggcagg tcgtggtcgc ggacgaatct cgtgatgcgg
      781 tcgttgacgg cggccatgga gaaaagcgag cccgcgccct ccttcgtctg catgccgccc
      841 gaggagatga agcagaccac cggcaagtgc tgctcggcgc actccacgag cagccggcag
      901 aatttctcgg cactggccat gtcgaaggag ccggcctgga aatccacgtt ggaaatcacc
      961 gttcccaccc ggacgtctcc gtcgccggtc ttgaagttac cgatgccggt aaccacgccg
     1021 cagggcggga tgccgcgatt cagggcttcc tcgatggaca accggaatcc cggaaactcg
     1081 cagggatccg agctcagaag atcggcgtct ctctcgtcga aggcggagaa gaacgtgtcg
     1141 acgaaggcct tgtaacccgt cgtgcggcgg ttcttggcat cgcgcagcac gttctggatg
     1201 agaaggtcac ggtacgcaat gttcagacgg ttccagaaat ccttgcgccg cccgatgttc
     1261 ttcggcgcga tccgcccgtt gtaagccttg ccctcgcgtt ccgactgcac gaaggaaagc
     1321 agcaggctgt cgaagaggtg cgtgaccacc acgaacaggg tgtcagagag gtgcggaaag
     1381 accgccttct tccattcctc gaccgccctg aagaaatcgg cacccttggg catccgcgcg
     1441 atgcgcagat accagtcgat gaactgccgg cgaagcttcg ccgtctcgga cgtctccgcc
     1501 ggctcgccac tgcctctgcc ccccagggtg actccggtag cggcaaggcc gagggtggtg
     1561 tcgatgagct gcgcaagcga tgtcttggcc agcgcctgcc gagactgggc ctcctcggct
     1621 atggaatcca ggttggcgat gatctggtca tagacggagt cgaacagcag gagattttcg
     1681 cactccgtca cgaagtcgcg ccggagatcg tccgagacga gcacgtccag cacggtcatg
     1741 tcgctgaagg gcaccggcgc accttccttt gccgttgcca tcaccaggcc cggatagcgc
     1801 tccaccaggt aggcgcggtt gcgcgccatg tccgatgatc tcgggctccc tgcctccggg
     1861 gctaccagcg gcccggtgat gaccaggttc agctcggcgg tgagctgtga gctcaaatac
     1921 agatccgatt tgtcctcgag gcgttcctcg ccgatgagct tcttgccttc gtgggtgaat
     1981 ccttcacgca ggcgcggcag cagcgcgtag tcggaccgca gcgccttgat caggccgcgg
     2041 gcgtcgggca ggtcgctgac cgttaggatg tgtcgaatgt cctcgtgacg tgtgagcatc
     2101 tccttgagag cgcgcaggtt gccaagggac tcggacttcc agcggttgta gaggaagacg
     2161 cccacggagg agatcaacag cgcccgggca tcctcgtagt tcttcttggg ctcaccgaag
     2221 agaaattgag caagccggcc gcgctcgtcg tgcaccgcct gcttcttgtc gctgtagttc
     2281 ttccaggcac gggaaagggc atcgtcgtaa acgacccggt ccggatcctg cagccatttg
     2341 aggaacagca ttcgccgttc gagctccgcc cgctgggtga gctcgccctg ggggagctcc
     2401 ctcaccgcca gacgcccgta ctggtgggtg ctggtggcct tgatacgctg tcgaaccttg
     2461 aggtaacgca gatagcggta cgccacgccg aagacgttga gatactccgt ggggttcttc
     2521 gtcagcttca tcgacgcgct ggcctgcatg accttcagct gctccgaagg gttcaggtag
     2581 cgctgcaggc tctgccggta gtggtcgagg atgtaggggt tctccgcgac gaactccttg
     2641 accttctctt cgacctgctg caccccggac acgatggcca ggcggaggtt ggccacgtcg
     2701 tcgtcgggca catagtcgat gaccccgtcg atgttgccct gggcatagag ctcgtagggc
     2761 gacaggccaa cgtacttggc gcactcctgc caggacaggt tcagccgacg cgcgatgttg
     2821 gccaggcttt tcggctggat ggtgctgaag accccgtcgc ggaccgacag gatcatgttg
     2881 ctggcggcca gcggtatcgc gccgcccgag tagccgagac catagatgat gccgacgttg
     2941 ggaacgtcca cgttgctcat ctcggcgatg agccgggaaa tgctgtgagc ctgattctcc
     3001 cggtttgcct gctcctcagc atcggcgccg ggcgtgtcca tcatcgtgac gatgggctgg
     3061 gagcgcttgg cgtagttctg gatgcggttc gcagcggcca gatgatgcgc cggcatccaa
     3121 acgccgttgc gggaggagcg atcctgggca atgaagccga ccaagcgcgg ctcgccgtcg
     3181 aaatccatct ccaccgtggc agcgtaaaag ggaccgtcgg aaacttcctc gacgacccgt
     3241 ccgagctgcc gaatgaccac gggcgccgcg gtgcggcccg gatcctccgc tcgcgcgacc
     3301 acggcctcga tgtaggcgtc cacgtcgaga ttggcgagcc gctgtttctg ccgcaggatg
     3361 tcacgcgggg acaacagccc cgggagccgt tcctggctct ccgcggtggg aaacagagaa
     3421 aagtcctgcg ccaggctttc cgcaatcagg tagagccgat caccttcgac gagctgttta
     3481 tccacgggcc gatcgttaag ggtgtagaat tcgccggtac tctagccact tgccaaatga
     3541 gaagcaatcc acgctcgcta agtggccgat ttttctagaa aattcgcatc tgcgccggag
     3601 cagcaaagga ggacgcggcc cgtgcccgac ctgacttccc tgaattgtgc cgtgctcacg
     3661 gtgtcggata cccgcacact ggaagacgac cgatcgggcg atctcctgat cgagttgctc
     3721 accgccgacg gtca
//
LOCUS       DBNHKV010000473         2366 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1272137, whole genome shotgun sequence.
ACCESSION   DBNHKV010000473 DBNHKV010000000
VERSION     DBNHKV010000473.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1272137"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /gene="cysC"
                     /locus_tag="AC16PP_15450"
     CDS             <1..771
                     /gene="cysC"
                     /locus_tag="AC16PP_15450"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007980946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HIR6483076.1"
                     /translation="EELPTRAFELNEIGRVTLSLDKAVPFEAYQDCHSMGAFVLVDRY
                     THATVAAGMINFALRRARNIHRQAVTVDKQARAKLNGHPPRVLWFTGLSGSGKSSITN
                     AVEQVLHLQGVRTYILDGDNVRHGLNRDLGFTETDRIENIRRIAEVAKLMVDAGVVVL
                     TAFISPFRAERDMARSLFDKGEFLEIFLDVPLEAAESRDPKGLYRKARRGELPNFTGI
                     DSPYESPETADLTLNTAELSVDDCVSRVLELLKDQGVS"
     gene            768..1016
                     /locus_tag="AC16PP_15455"
     CDS             768..1016
                     /locus_tag="AC16PP_15455"
                     /inference="COORDINATES: protein motif:HMM:NF021362.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HIR6483077.1"
                     /translation="MTASDLPQTESHLRSVAKAFSWRVVATLTTMTIAWMITGEVDTA
                     IMIGGFEFVLKIFIYYLHERAWQLVPRGAVRRIARLSS"
     gene            complement(1006..1980)
                     /locus_tag="AC16PP_15460"
     CDS             complement(1006..1980)
                     /locus_tag="AC16PP_15460"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6483078.1"
                     /translation="MTSNRFPDPAMIFTNGIRMAVYQDGPADGFPVVLCHGFPELAYS
                     WRHQLQALADAGFHAIAPDQRGYGRTDKPDDAGAYDIHHLTGDLAGLLDAMGLERAVF
                     CGHDWGGLVVWQMALLHPSRVAGVIGAGVPFLPRAEEDPVETFRRMWGERMYIVNFQT
                     SHEADELFDADPERVFQVLMRKNPMSLAEYAELPLQERVADLIARVKAGPSSTPSIIS
                     EEELRVFASAFRAGGFTGPINWYRNWSRNWSTTAHVPQKIEVPALYIKAGNDLATAAI
                     PDLESRMAGHVADLEIREVPESGHWLQQEFPDEVSGFTVDWLTRRFTS"
     gene            2062..>2366
                     /locus_tag="AC16PP_15465"
     CDS             2062..>2366
                     /locus_tag="AC16PP_15465"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6483079.1"
                     /translation="MTTRNDDWHEMMVPFPAPRFESVNGIRIACYEMGEGRPVVLCHG
                     FPELAFSWHRVMPRLADAGYRAIAIDQRGYGASDCPPAVEDYAITEIAADVAGLLDAL
                     "
BASE COUNT          411 a          773 c          770 g          412 t
ORIGIN      
        1 gaggagctgc cgacccgggc tttcgagctc aacgagatcg gtcgggtaac gctcagcctg
       61 gacaaggccg ttcccttcga ggcttaccag gactgtcact ccatgggcgc gttcgtgctc
      121 gtcgatcggt acacgcacgc caccgtcgcc gccgggatga tcaacttcgc cctgcgtcgg
      181 gctcgcaaca ttcaccgaca ggccgtgacg gtcgacaagc aggcgagggc gaagctcaac
      241 ggccacccgc cccgcgtcct ctggttcacg ggcttgagcg gttccgggaa atccagcatc
      301 accaatgcgg tggagcaggt cctgcacctg cagggggtgc gcacgtacat cctcgacggg
      361 gacaatgtcc gccacgggct gaaccgggat ctcggcttca ccgaaaccga ccggatcgag
      421 aacatccgcc ggattgccga ggtcgccaag ctcatggtgg acgcgggcgt cgtggtgctc
      481 accgcgttca tctcgccctt ccgggcggag cgggacatgg cccggtcact gttcgataag
      541 ggcgagttcc tggagatctt cctggacgtt ccgctggaag cggcggagtc gcgcgacccc
      601 aaaggcctct accgtaaagc ccgtcgcgga gagttgccga acttcaccgg catagacagc
      661 ccttacgagt cgccggaaac ggccgacctc acgctcaaca cggccgagct cagcgtggac
      721 gattgcgtct cgcgggtcct cgagcttctc aaggatcagg gggtttcatg acagcgtccg
      781 acctaccgca aaccgagtcg cacctgcgca gcgtcgccaa ggctttttcc tggcgggtgg
      841 tggcgacgct caccaccatg accatcgcct ggatgatcac cggcgaggtg gacaccgcca
      901 tcatgatcgg cggcttcgag ttcgtgctga agatcttcat ctactacctg cacgagcgcg
      961 cgtggcagct ggtgccgcga ggggcggtgc gcaggatcgc gcgtctcagc tcgtgaagcg
     1021 ccgggtcaac caatccaccg tgaaaccgct cacctcgtcc ggaaattcct gctgaagcca
     1081 gtgcccgctc tcgggcactt cgcggatctc caggtcggca acgtgcccgg ccatgcgtga
     1141 ctccaggtcc gggatggcgg cggtcgccag atcgttgccg gccttgatgt agagtgcggg
     1201 cacctcgatc ttctgcggca cgtgggcggt cgttgaccag ttgcggctcc agttgcggta
     1261 ccagttgatc ggcccggtga acccgcctgc ccggaaagcg gacgcgaaga cgcggagctc
     1321 ctcctcggag atgatgctgg gcgtgctcga cggtccagct ttcacccgcg cgatcaggtc
     1381 ggccactcgc tcctggagcg gaagctcggc gtattcggcc aggctcatcg ggttcttgcg
     1441 catcagaacc tggaagacgc gctccggatc cgcgtcgaac agctcgtctg cctcgtggct
     1501 cgtctggaaa ttcacgatgt acatgcgttc gccccacatg cgtcggaacg tttccacggg
     1561 gtcctcttcc gccctcggaa gaaacggtac tccggcgcca atgacgccgg cgacgcggga
     1621 cgggtgcaga agcgccatct gccagaccac cagcccaccc cagtcgtgcc cgcagaacac
     1681 ggcacgctcc agccccatgg catcgagcag gccggccaga tcgccggtca ggtggtggat
     1741 gtcgtacgcc cccgcgtcat cgggcttgtc cgtgcggccg tatccccgct ggtcgggcgc
     1801 gatcgcgtgg aagcccgcgt cggccagcgc ttggagctga tgacgccagg agtaagcgag
     1861 ttccggaaag ccgtggcaga gcaccaccgg gaagccgtcc gcaggaccgt cctggtagac
     1921 ggccatgcga atgccgttcg tgaagatcat cgcgggatcg ggaaaccggt tgctcgtcac
     1981 ggatcatctc tgctgtcagc gctgcgaacc caaccatcgc gcgagacagg gccaggcgca
     2041 aggggcgttc gtgagggtaa gatgacgacc cggaacgacg attggcacga aatgatggtt
     2101 ccttttccag cgccccgatt cgaaagcgtc aacggcattc gtatcgcctg ctacgagatg
     2161 ggggaggggc gtcccgtggt gctctgccac ggctttccgg agcttgcgtt ctcatggcac
     2221 cgggtgatgc cgaggctggc ggatgccggc tatcgcgcca tcgccatcga tcagcgcggt
     2281 tacggggcca gtgactgccc gccggcggtg gaagactacg ccatcactga gatcgccgcg
     2341 gacgtggctg gcctgctcga cgccct
//
LOCUS       DBNHKV010000474         6496 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_65036, whole genome shotgun sequence.
ACCESSION   DBNHKV010000474 DBNHKV010000000
VERSION     DBNHKV010000474.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_65036"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1303)
                     /locus_tag="AC16PP_15470"
     CDS             complement(140..1303)
                     /locus_tag="AC16PP_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009686373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HIR6483080.1"
                     /translation="MSAAEFDFGFDETLNMLRDSVRQFAADEIAPRAAQIDASNEFPR
                     DLWPKLGALGLLGVTVEEAYGGAGLGYLAHCIAMEEISRASASVGLSYGAHSNLCVNQ
                     IRRYATEEQKQRYLPPLISGDYLGALAMSEPGAGSDVVSMRLRAEDRGDSFVLNGSKM
                     WITNGPGADVLVVYATVDPSLGPRGITAFLIDKGTPGFSTAQKLDKLGMRGSDTCELV
                     FDDCEVSKTQVLGEVGQGVRILMSGLDYERVVLAAGPLGIMQSCLDTVLPYVHEREQF
                     GQPVGTFQLMQGKLADMYTTMNACRAYVYAVAQACDAGRTTRFDAAGCILFAAEKATW
                     MASEAVQALGGNGDITDYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFDATG"
     gene            complement(1326..3254)
                     /locus_tag="AC16PP_15475"
     CDS             complement(1326..3254)
                     /locus_tag="AC16PP_15475"
                     /EC_number="6.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HIR6483081.1"
                     /translation="MFDTLLIANRGEIACRVMRTATRMGLRTVAVYSDADAHAQHVRL
                     ADAAERIGPAPARESYLRIDAVLDAAERHGAGAVHPGYGFLSENAEFAAACEERGIVF
                     VGPSARAIRAMGSKIEAKRIVAAAGTPVVPGYQEADQSDAALSRAAAEIGYPLLIKAS
                     AGGGGKGMRLVREPDEFPAALAGARREAAAAFADELVLLERYLDAPKHLEVQILADQT
                     GHTLHLYERDCSVQRRHQKVIEEAPGPTVSEELRTLLGEAAVQAAKAIGYVGAGTVEF
                     ISQAGEFYFMEMNTRLQVEHPVTEAITGLDLVEWQLRVAAGEKLPFRQHEIPRNGHAV
                     EARVYAENPARRFLPSTGTLAHVAFPQEVRVDAGVATGDAVSVHYDPMLAKVIAHGPD
                     RDAAITSLERALARTEIAGVAHNVAYLRRLLAHPVFRGGDYTTGIAEEHADELLAEED
                     HLPAICAAIAAGRLGDSVGPWARRDGFRLNGPASFIQRFRRERKEVEVVLSEGQAQVD
                     GEVFDVADVSWNEGVLALRVDGNRVGARVVTAGPSLYVIIGGSTARFDPGDVEGAARS
                     LGSAAGNRVVSPMPGQVIAVSVKPGDRVKAGAVLAVVEAMKMEHSVAAPRAGRVASVN
                     CRVGDRVDEGVELVVLEE"
     gene            complement(3257..4867)
                     /locus_tag="AC16PP_15480"
     CDS             complement(3257..4867)
                     /locus_tag="AC16PP_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017709070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR6483082.1"
                     /translation="MAILQSNIDTGAAAFAANAKHHRELADELRTLAGRIVEGGDARS
                     RERHVGRGKLLPRDRLSRLLDPGSPFLEVGQLGGYELYDDWIPSGGIVTGIGRVSGTQ
                     CMVVVNDATVKGGTYYPITVKKHLRAQEIALENGLPCVYLVDSGGAFLPMQAEVFPDR
                     DHFGRIFYNQANLSARGIPQIAVVMGSCTAGGAYVPAMCDQAIIVRGQGTIFLGGPPL
                     VKAATGEEVDAESLGGGDVHSRTSGVTDYLAADDVHALGIAREIVAASQQRYRATVET
                     RTPRPPAFAEEELYGVLPTDPRTPVDVREIIARLVDGSEFHEFKALYGETLVCGFAHI
                     EGMPVAVIANNGILFSESALKGAHFIQLADRRGVPLLFLQNITGFMVGKKYENQGIAK
                     DGAKMVTAVACARVPKFTVVIGGSFGAGNYAMCGRAYGARMLWTWPNARISVMGGEQA
                     AHVLATVRRDGAAARGEEWPEAEQAAFMDDIRAQYETQGHPYFASARLWDDGVVDPAD
                     TRRVLGLALEVACREPLQPQSGFGVFRM"
     gene            complement(4958..6343)
                     /locus_tag="AC16PP_15485"
     CDS             complement(4958..6343)
                     /locus_tag="AC16PP_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HIR6483083.1"
                     /translation="MADPLHNQFPPGSLEARDLEHLLHPTTNLEQHHKVGPRVHDRAE
                     GVYLWDSQGRQYLEGMAGLWCTALGYGEEELVSAAAQQMRKLSYSQLFGGRSNEPSIL
                     LAERLKAMAPFDAGRVFFGLSGSDANDTQIKLMWYYHNAIGKPEKKKIISRMRGYHGV
                     TVAAGSLTGLPAFHAHFDLPIPGVLHVDAPHYYREAKGGETETEFVDRLVGDLEELIL
                     GEGPETIAAFIAEPVQAAGGVIVPPPGYFAKVQALLARHDILFIDDEVICGFGRTGRA
                     FGAQTFGIEPTTMSVAKGVSSAYLPLSAVIIPEFMYEPLVELSGSVGTFGHGFTYSGH
                     PVCAAVALRTLELMEEREIFAHAAAVSEPFQERLALLADHPLVGDARGVGLLGAVELV
                     ADKDTRTPFPPGQGVGAHCQERCESHGLILRALGDVVAICPPLIISEDQVDELFAKLE
                     RALDDTFAWVH"
BASE COUNT         1151 a         2260 c         2020 g         1065 t
ORIGIN      
        1 ggccaggcca atcagtaaga acttcatacc cttcgatctg acaaccaggc ttcgcaaatc
       61 tctttctcca ggtgagatgg gcggaaaaaa catgccgaga gggcagcgca agctgccggg
      121 cgcgaaatta tagccaaggt catccggtgg cgtcgaacaa ctcccgaccg atgagcatgc
      181 ggcgaatctc gctggttccc gcaccgatct cgtagagttt cgcatccctg agcagccgcc
      241 cggtgggata gtcggtgatg tcgccgttgc cgcccagggc ctgcaccgct tcgctggcca
      301 tccaggtggc cttctcggcg gcgaacagga tgcagccggc cgcgtcgaaa cgcgtcgttc
      361 gcccggcatc gcacgcttgc gcaaccgcgt agacgtatgc ccgacaggcg ttcatggtgg
      421 tgtacatgtc cgcgagcttt ccctgcatga gctgaaaggt gcctaccggc tgtccgaact
      481 gctctcgctc gtgcacgtag ggcagcacgg tatcgaggca gctttgcatg atgcccagcg
      541 gcccggccgc aagaaccacc cgttcgtagt ccagcccgct catcagaatt cgaaccccct
      601 ggcccacctc tccgagcacc tgggtcttgg acacctcgca atcatcgaag acgagctcac
      661 aggtatccga tccccgcata cccagcttgt cgagtttctg agcggtgctg aaacccggcg
      721 tgcccttgtc gatgagaaac gccgtgatcc cgcgaggacc cagactcggg tccaccgtgg
      781 cgtaaaccac cagaacgtcc gcccccggac cgttggtgat ccacatcttg ctgccattga
      841 gcacgaagct gtcgccccgg tcctcggctc gaagcctcat gctgaccacg tcggagcccg
      901 cgcccggctc gctcatggcc agagcaccca ggtaatcccc ggaaatcagc ggcggcaggt
      961 agcgctgctt ctgctcctcg gtggcgtagc gacgaatctg attgacgcaa aggttcgagt
     1021 gggcgccgta ggacagcccg acggaagccg acgcccgact gatctcttcc atggcgatgc
     1081 agtgcgccag gtagccgagc ccggcgccgc cgtaggcttc ctccacggtg acaccgagca
     1141 ggccgagggc gcccagcttg ggccagagat ccctgggaaa ctcgttgctg gcatcgatct
     1201 gcgccgcgcg gggcgctatc tcatcggccg cgaactggcg cacgctgtca cgcagcatgt
     1261 tcagggtctc gtcgaacccg aaatcaaatt ctgcagcact catgacacac ggtcctccca
     1321 ggaattcatt cctcgagcac gactagctcg acgccttcgt ccacccgatc accaacgcga
     1381 cagttcacgg atgccacccg gccggcacgc ggagcggcaa cggagtgctc catcttcatg
     1441 gcttcgacga cggcgagaac ggcgcccgcc ttcacccggt caccgggctt caccgagacc
     1501 gcgatgacct gcccgggcat gggcgatacg acccggttac ccgcagccga accgagggag
     1561 cgagccgccc cttcgacgtc gcccggatcg aaccgggccg tcgacccgcc aatgatgacg
     1621 taaaggctgg gcccggccgt caccacccgg gctccgacac ggttgccatc cacccgcagc
     1681 gccagcacgc cctcgttcca gctgacatcg gccacatcga aaacttcgcc gtcgacttgg
     1741 gcctgaccct cggacagcac gacctctacc tcttttcgtt cgcgtctgaa acgctggata
     1801 aaggaggcag gcccgttcaa ccggaacccg tcgcgtcggg cccagggccc gacgctgtcg
     1861 ccgagccgcc cggccgcgat ggccgcgcag atggcgggca ggtgatcttc ctcggcgagg
     1921 agctcgtccg catgctcctc ggcgataccc gtcgtgtagt cgccgccccg aaagaccgga
     1981 tgggccagga gccgccgcag gtaggcgacg ttgtgcgcca cgccggcaat ctcggtgcgc
     2041 gccagggccc gctcgaggga ggtgatcgcg gcgtcacgat cgggcccgtg ggcgatcacc
     2101 ttcgcaagca tggggtcgta gtgaacgctc acagcgtccc cggtggccac ccccgcatcg
     2161 acacgcacct cttgcggaaa agccacatgg gcaagggttc ccgtcgaagg aagaaaacgc
     2221 cgggccggat tctccgcgta aacgcgcgct tccaccgcgt ggccgttacg cggtatctca
     2281 tgctgtcgga aaggcagctt ttcgcccgcg gcaacccgca gctgccactc gacgaggtcc
     2341 agcccggtga tcgcttccgt gaccggatgc tcgacctgga gccgcgtgtt catctccatg
     2401 aagtagaact cgcccgcctg cgagatgaac tccacggtgc ctgctccgac gtagccgatg
     2461 gctttggccg cctgcacggc tgcctccccg agcagggtac gcagctcctc cgaaaccgtc
     2521 ggcccgggcg cctcctcgat cactttctgg tggcgcctct gcaccgagca gtcgcgctcg
     2581 tagaggtgga gcgtgtggcc ggtttgatcc gcgaggatct gcacctcgag gtgcttcggc
     2641 gcatccaggt agcgctcgag cagcacgagc tcgtctgcaa aggccgccgc cgcttcgcgc
     2701 cgggcaccgg ccagggcagc cgggaactca tcgggttccc gcaccagacg catccccttg
     2761 ccgccgcccc ctgccgaggc cttgatgagc agcgggtaac cgatctcggc cgccgcgcgg
     2821 gacagcgccg catcgctctg atccgcttcc tgatagcccg gcacgacggg cgttcccgcc
     2881 gcggccacga tgcgtttcgc ttcgatcttc gagcccatgg cgcgaatggc gcgggccgaa
     2941 gggcccacga aaacgatgcc gcgctcctcg caggcagcgg caaactccgc gttctcggag
     3001 agaaacccgt agccggggtg aacggcgccc gccccatggc gctccgcggc gtcgagcacc
     3061 gcgtcgatgc gcaggtagct ctcgcgggcc ggtgccgggc cgatccgctc ggccgcgtcc
     3121 gccagacgca cgtgctgcgc gtgggcgtcg gcatcggagt acacggcgac cgtgcgtaag
     3181 cccatgcgcg tggcggtgcg catcaccctg caggcgatct cgccacgatt tgctatcagc
     3241 agagtatcga acatggctac atcctgaaga cgccgaagcc ggactgaggc tgcagcggtt
     3301 cccggcaggc aacctcgagc gcgaggccca gcacgcgccg ggtatcagcc gggtcgacga
     3361 cgccgtcgtc ccagagcctg gcgctcgcga agtagggatg tccctgagtt tcgtactgag
     3421 cacggatgtc gtccatgaac gccgcctgct cagcctccgg ccactcttcc ccgcgagcgg
     3481 ctgccccgtc ccggcgtacg gtggccagca cgtgggccgc ctgctctccc cccatgacgg
     3541 atatccgggc gttcggccac gtccagagca tgcgcgcgcc gtaggcgcgc ccgcacatgg
     3601 cgtagttgcc ggcaccgaaa gagccaccga tcacgacggt gaatttcggt acccgcgcgc
     3661 aggccaccgc cgtgaccatc ttcgccccgt ctttcgcaat gccctgattc tcgtacttct
     3721 tgcccaccat gaagccggtg atgttctgca gaaagagcag aggcaccccg cgccggtcgg
     3781 cgagctgaat gaagtgcgcg cccttgagcg ccgactctga gaacaggatg ccgttgttgg
     3841 cgatcacggc aaccggcatc ccctcgatgt gggcaaaccc gcagaccagc gtttctccat
     3901 acagcgcttt gaactcgtga aattccgatc cgtccaccag ccgggcgatg atctcgcgca
     3961 cgtccaccgg ggtgcgcgga tccgtgggca gcaccccgta gagctcctcc tcggcaaagg
     4021 ccggaggtcg gggcgtgcgg gtctcgaccg tcgcgcgata gcgctgctgg ctggccgcga
     4081 cgatttcgcg cgctatgccc agggcatgga catcgtccgc cgccagatag tcggtcacgc
     4141 ccgacgtgcg gctgtgcacg tcaccgccgc caaggctttc cgcatccacc tcctcgccgg
     4201 tcgcggcctt taccaggggc ggcccgccga gaaagatcgt cccctggccc cggacgatga
     4261 tggcctggtc gcacatggcc ggcacgtaag cgccccccgc cgtgcaggat cccatcacca
     4321 ccgcgatctg ggggatgccg cgagcagaga gattggcctg gttgtagaaa atccgaccga
     4381 agtgatcccg gtcgggaaag acctccgcct gcatgggcag gaacgcgccg ccggaatcca
     4441 ccagatagac gcacggcaag ccattctcca gcgcgatctc ctgcgccctc aagtgcttct
     4501 tgacggtaat cgggtaatac gttccgcctt tgacggtggc gtcattcacc accaccatgc
     4561 actgcgtgcc cgagaccctg ccgatgccgg tgacgatgcc gccgctggga atccagtcgt
     4621 cgtaaagctc gtagccgcca agctggccaa cctccagaaa tggtgagccc ggatccagca
     4681 gacggctgag gcggtcccgc ggcagaagct tgccgcgacc aacgtgacgc tccctggagc
     4741 gggcgtcccc gccctcgacg atgcgcccgg caagcgtcct caactcatcg gcaagctcgc
     4801 ggtggtgctt cgcgttggcg gcgaacgcgg cggcgccggt atcgatattc gattgcagaa
     4861 tggccataac tccccccaga cggcaaagat acacaacgac gtcaacccaa tgaactgcct
     4921 gccaaggcgc cgcagctgtg acgcgaacgc tcgctgttca gtgaacccag gcgaacgtgt
     4981 cgtccagcgc cctttccagt ttcgcgaaga gctcgtcgac ctgatcctcg gatatgatca
     5041 gcggcgggca gatggccacc acgtctccga gcgctcggag gatcaacccg tgggactcgc
     5101 aacgttcctg gcaatgcgcg cccacaccct gcccgggagg aaagggcgtg cgggtgtcct
     5161 tgtccgccac gagctccacc gcgccaagga gcccgacccc gcgcgcgtcc cccaccagtg
     5221 ggtggtcggc cagcaacgcc agacgttcct ggaacggctc cgacacggct gctgcgtgag
     5281 caaagatctc gcgctcctcc atgagctcta gcgtccgaag cgcaaccgcc gcacacaccg
     5341 gatggcccga ataggtaaag ccgtggccga aggttcccac gctcccgcta agctccacca
     5401 gcggttcgta catgaactcg ggaatgatca cggcgcttag cggcaggtag gccgaagaga
     5461 cgcccttcgc cacgctcatg gtggtcggct cgatcccgaa cgtctgcgca ccgaacgcgc
     5521 ggcccgtgcg gccgaagccg cagatgacct cgtcgtctat gaagaggatg tcatgacggg
     5581 ccaacagcgc ctgcaccttg gcgaagtagc cgggcggcgg cacgatcacc ccgccggcgg
     5641 cctgaacggg ttccgcgatg aacgcggcaa tggtttcagg gccctcgcca agaatcagct
     5701 cctccagatc gccgaccagc cggtcgacga actcggtctc ggtctcgccg cccttcgctt
     5761 cgcgatagta gtgtggagca tcgacgtgca gcacccccgg gatcggcaga tcgaagtgcg
     5821 cgtggaaggc gggaagaccg gtcagggacc cggcggccac cgtcacgccg tgatagccgc
     5881 gcatgcggct gatgatcttc ttcttttccg gcttgccgat ggcattgtgg tagtaccaca
     5941 tgagcttgat ctgggtgtcg ttggcatcgc tgcccgagag accgaagaag actctaccgg
     6001 catcgaaggg ggccatggct ttcaaccgct cggccaggag tatgctgggc tcgttgctgc
     6061 ggccgccgaa cagctgcgag tagctgagct tgcgcatctg ctgcgcggcc gcgctgacga
     6121 gctcttcctc cccgtagccg agcgccgtgc accaaaggcc cgccatgcct tcgagatact
     6181 gacggccctg ggaatcccag agatagacgc cttcggcccg gtcgtggaca cgaggaccca
     6241 ccttgtgatg ctgttccaga ttcgtcgtgg ggtggagaag gtgctccaga tccctggcct
     6301 ccagggagcc gggcggaaac tgattgtgca gcggatcggc catgaacgtc tcccgacagg
     6361 cggaaggcga agcttacggg gcggcgggtc cgagcgctac ggcaaagcag cgcggtcgca
     6421 gcgcccgcgc tgcgcctgcc gccattgcgt tacactgcgc ctcatgctcg cccgaaccgc
     6481 gattatcacc ttctcg
//
LOCUS       DBNHKV010000475        10807 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1059952, whole genome shotgun sequence.
ACCESSION   DBNHKV010000475 DBNHKV010000000
VERSION     DBNHKV010000475.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 10807)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10807)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10807
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1059952"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="AC16PP_15490"
     CDS             <1..266
                     /locus_tag="AC16PP_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6483084.1"
                     /translation="ALRYVDDMELTFLNSPQSSVGSHTVLDASLTYTVNQLSISVWGL
                     NLTEDDSWTQAYDVGTSPAFAGLWTYTAIRPPRTYGAVVSWDF"
     gene            complement(371..1990)
                     /locus_tag="AC16PP_15495"
     CDS             complement(371..1990)
                     /locus_tag="AC16PP_15495"
                     /inference="COORDINATES: protein motif:HMM:NF013355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HIR6483085.1"
                     /translation="MTIRFLGKNVSGPFTIPSGIVATAVPIIEYLFRHVPQIGVMTTK
                     SIGLAPRTGNREPIYSQYAPGCFVNAVGLTNPGAERSAELFAALDVPEDRFLLTSIFG
                     GSVEEFVAVAKILAPVSDGLELNLSCPHAKGYGMEMGRDPELVREITAAVKAAVDVPV
                     VPKLTPNADNIGEIAQAAVAGGADALCAINTVGPGYYSAAGEPVLSNELGGMSGKGIL
                     PIGLKCVREIAGRVNVPVIGCGGISSAADVNAYRAAGAHIFGVGSALTGMTSPEITQY
                     FQTLAADLADDGNDAEARIQYRLDMGFRPYTLVTNTRVTDDICILTFDRPLDIKAGEF
                     VFLWIPGLGEKPFSALTDDPFSLVVIDLGQFTHALMDLAPGTRTYVRGPHGQPAQPPP
                     GARIVAVAGGTGLAAVYQLARDHGNADIFMGARTAERLYFIDECEAAATLHVATDDGS
                     CGHHGLVTELLAKHLETLSENELANTVFYNCGPEPMVHAAVEVQRRFCPDEQIFSAID
                     YLTKCGVGICGACTAPDGRRLCVDGPFLSAS"
     gene            complement(2065..2898)
                     /locus_tag="AC16PP_15500"
     CDS             complement(2065..2898)
                     /locus_tag="AC16PP_15500"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6483086.1"
                     /translation="MGKLEGKVAIVTGAGRGIGRAIALKLAAEGAHVVVNDLDEQPAG
                     AVTDEINSGTGPRALAFPGDVTLPEFGDDLVDAALDGFGGLDVLVNNAGYIWNSALLN
                     HSDEQWEAMLAVHATAPFRILRAAGRHFREQAKAAQARGERPPCRKVVNVSSISGVYG
                     AATQASYAAGKAAVIGLTKSLSKEWGPYNVTVNAVAFGYIETRLTQTIGDEVETIDVK
                     GRQHRVGLTPEAIEAYRSTSPLRRAGAPEDAANAVALFCFPESDFVTGEVLIASGGTT
                     G"
     gene            complement(2927..3433)
                     /locus_tag="AC16PP_15505"
     CDS             complement(2927..3433)
                     /locus_tag="AC16PP_15505"
                     /inference="COORDINATES: protein motif:HMM:NF025050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6483087.1"
                     /translation="MQVAYHVTDIHRAAREMAEKVGAGPFFINENITLTWGEHRGEPT
                     DFVHSSAYGQWGELMVEFFQQENDTPNTPYRDMYGPDEEGLHHTAIMVPDMEEAFEYF
                     ERNGMPVVTRCGLGRSGGVDFAFIDARKLLGHMIEIYPRSDGVVGFYRHVREASVGWS
                     GEDPLRSV"
     gene            complement(3461..4216)
                     /locus_tag="AC16PP_15510"
     CDS             complement(3461..4216)
                     /locus_tag="AC16PP_15510"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HIR6483088.1"
                     /translation="MIDDYSDRHHRFEGCFNFRDIGGYPTTEGRTVRWGRYYRAGRQD
                     RMSEADVARARDLGIATQIDLRKPDEVREQGRGPLEEMGVAYHHIPVIPEGGSDALSR
                     LVGDTGISGRRYLGYLEFGPEPWLAMFEIFADAGRHPIVLHCTAGKDRTGVSTAFLLS
                     VLGVDRALIEADYILTNRDVGRQADFIERTVGYPEGMDRAAMVHAAGVPETAIGDFLD
                     GLEARWGGPLGYLRSIGVSEATMDDVRREFLED"
     gene            complement(4518..5837)
                     /locus_tag="AC16PP_15515"
     CDS             complement(4518..5837)
                     /locus_tag="AC16PP_15515"
                     /inference="COORDINATES: protein motif:HMM:NF016873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HIR6483089.1"
                     /translation="MRSLKDLLVVIPHSGIRIPPEIPSDSLTEEFPALLANVDWHTDR
                     LYDFTDLLENQQIVFPYCSLLLEANRDPELLDESIPLRDVNGKAVYRPGCEPSLDLRR
                     HLSYKYLRGFHQKISASISAGAEFLLDGHSTVVARGVAANQIDLMNFQHTQRDGEPKL
                     YAPAIYAETYAEALQKRLPEVHVTVNASEYYQVHGHVCAAHSVNGIGRAGQLAPALIQ
                     ETSQGLYMHPDGTPDVLAVDHLRRVFAEALRTTLDTVRRFTAPAQIIDLHGQRQTFDF
                     DCGVKALQMVLAYYGVEEREDKLLKALAADPELGTPLARMIEFAESRGFEVKAGTGWS
                     LDDVKHYLDRNCPVIVLVQAWADGYLSLAEWRRNFDDGHYVVVVGYDRQNLYFEDPAS
                     FHRTWLKENEFLARWHDQDPVTGEKLLQAGMVLLGREPVGKGLRPMY"
     gene            6313..7500
                     /locus_tag="AC16PP_15520"
     CDS             6313..7500
                     /locus_tag="AC16PP_15520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HIR6483090.1"
                     /translation="MQRRAKVVRLRTAGRGNPRTREFRIRHFPDVSDAEWNDWRWQSR
                     HRFKNLAQIERVLELSDDERAALTVGGMMLPVGITPYYMSLLDPVDPQHALRRTVIPS
                     QNEFLRLPGEADDPLGEDAHSPVPGLVHRYPDRVLMLPIDFCSTYCRYCTRSRVVGHG
                     ELSPQTSRLEAMFDYLRGATQVRDVLVSGGDALALGEDNLDYILSNLRAIEHIEFVRI
                     GTQMPAVLPQRITRDLTRVLRKYHPLWMSLHFLHPEECTPESTQACERLADAGIPLGS
                     QTVLLKGVNDDVGVMTRLVHGLLKMRVRPYYLYQCDPISGSAHFRTTVEKGLEIIEGL
                     RGHTTGYAVPTYVIDAPGGGGKIPVQPNYVLGRDVDELKLRNFEGRVFSYPDPQQPGA
                     GTN"
     gene            7503..8516
                     /locus_tag="AC16PP_15525"
     CDS             7503..8516
                     /locus_tag="AC16PP_15525"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR6483091.1"
                     /translation="MRIGITYDLRSAYLAAGYSEEETAEFDRDDTVDAIEAALRQLGH
                     ETVRIGCARELIEHLAAGQRWDLVFNICEGLSGPGRESQVPAILEVYGIPVTFADTAV
                     MALSLNKAWAKTVVADAGVATPQHAVIARAADVATVALEPPLFVKPIGEGTGKGTSAA
                     SVVMSAAELERQSLALLERYRQPVLVETYLPGREFTVGLLGTGPNARVLGTLEIELLA
                     TAEANVYSYLNKEECESRVLYRLVDGRADPVVARAEAAALGAWRALGARDGGRIDLRA
                     DSAGEPNFIEANPLAGLHPAHSDLPMLATAVGMPYAELIRAIVDSASERLAMDATEMN
                     RCA"
     gene            8507..9493
                     /locus_tag="AC16PP_15530"
     CDS             8507..9493
                     /locus_tag="AC16PP_15530"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR6483092.1"
                     /translation="MRVAIAYNKPGDAAGSAEADVLEQVEAVETALVGAGHQVCALPC
                     TLDLAGFDGALAKQRPDVVFNLVEALGGTDRLAVLVTLLLDARGVPYTGAGSRALELS
                     ADKVGAKERMRRLGLPTPDWYVCEKTQVDFPGAGCYIVKARFEHASLGLDDQSVVQVA
                     DAGQLTRRIRARGKHSGAQQFAERYVAGREFNVALLASETGPAQVLPPAEIDFSAFAP
                     GKPRIVGHDAKWSSRAFEYHATPRRFDFPAADARLLDLLGTLGMQVWECFAMRGCARV
                     DFRVDEDGRPWILEINPNPCLSPDAGFAAALDAAGIPAEEMVGRLVRTAATD"
     gene            9496..10035
                     /locus_tag="AC16PP_15535"
     CDS             9496..10035
                     /locus_tag="AC16PP_15535"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6483093.1"
                     /translation="MTSPCKEHPPGDAWVHLREPAARDRGPVASVLVSTGFFSDAEVA
                     VALEVFDAALQGKDSGYEHLVAESAGRVIGYVCWGGPIEMTLSSFDLYWIAVDAAARG
                     RGVGHRLLAAAESLAAASGCAQLFAETSGRADYAPTHSFYESCGYLRAAELADFYAPG
                     DAKVFYVKRLTGGNQAPPE"
     gene            complement(10070..>10807)
                     /locus_tag="AC16PP_15540"
     CDS             complement(10070..>10807)
                     /locus_tag="AC16PP_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HIR6483094.1"
                     /translation="GDRRKAMLQDLAILTGGKVISSDAGFSLEKVALEDLGEAQRVLL
                     TKGDTTLIGGRGGTDAIAGRISQIRREIEESKSEYDREKLQERLAKLAGGVAVINVGG
                     ATEVAMKERKMRVDDALAATRAAVEEGVVPGGGVALLRASAELAKAAPRNQDQASGVD
                     ALRRAAEAPLRQIVANAGLQPGVVLRKIQSEQGHFGFNAQTETFGDMLEMGILDPCKV
                     VKSALHNAASVAGMMMSTEALVADAPR"
BASE COUNT         1761 a         3564 c         3557 g         1925 t
ORIGIN      
        1 ccgccctgcg ctacgtggac gacatggagc tgacgttcct gaactcgccc cagagctcgg
       61 tgggctccca cacggtgctg gatgcgtcgc tgacctacac ggtgaaccag ctcagcatca
      121 gcgtctgggg gctgaacctg accgaggacg actcctggac ccaggcctac gacgtgggta
      181 ccagccccgc ctttgccggg ctctggacct acacggccat ccgtccgccc cgcacctacg
      241 gcgcggtggt cagctgggac ttctagcttt cggcagtgcc ggcacgaaaa gccctcttcg
      301 gagggctttt tttctgccca agggtaggcg ctgatcggcc tctggggagg aacgcgcagt
      361 gggctttgcg tcagctcgcg ctcaggaacg ggccgtccac gcacaggcgg cggccgtcgg
      421 gtgcggtgca ggcgccgcag atgccgaccc cgcatttggt gaggtagtcg atggcgctga
      481 agatctgctc gtccgggcag aaccggcgct ggacctcgac cgccgcgtga accatgggct
      541 ccgggccgca gttgtagaag acggtgttgg cgagctcgtt ttccgacagc gtttccaggt
      601 gtttggccag cagctcggtc acgaggccat ggtgcccgca gctgccgtcg tcggtggcca
      661 cgtgcagggt ggccgccgct tcgcactcgt cgatgaagta gagccgttcg gccgttcgcg
      721 cacccatgaa gatatcggcg ttgccgtggt ctcttgccag ctggtagacg gcggcgaggc
      781 ccgtaccgcc ggcgaccgcg acgatgcgcg cgcctggggg cggttgcgcc ggctggccgt
      841 ggggaccgcg cacgtaggtc ctggtacccg gagcgagatc catgagcgcg tgggtgaact
      901 gccccaggtc gatgaccacc agcgagaacg gatcgtcggt cagcgccgag aaaggctttt
      961 cgcccagacc gggtatccac aggaagacga actcacccgc tttgatgtcc agcggccggt
     1021 cgaaggtgag aatgcagatg tcgtcggtga cgcgggtgtt cgtcacgagc gtgtacgggc
     1081 ggaagcccat gtcgagccgg tactggatcc tggcttccgc gtcgttcccg tcgtctgcca
     1141 ggtctgccgc cagcgtctgg aagtattggg tgatctctgg cgacgtcatg ccggtcagcg
     1201 ccgagcccac cccgaatatg tgggcccctg ccgcgcggta ggcgttaacg tcggccgcgc
     1261 tgctgatccc accgcagccg atcacgggga cgttgactct tccggcgatc tcccgaacgc
     1321 acttcaggcc gatgggcagg atgcctttgc ccgacatgcc gccgagctcg ttgctcagca
     1381 caggctcgcc tgcggcgctg tagtagccgg gccccacggt gttgatggcg cacagggcgt
     1441 ccgccccgcc ggccacggcg gcttgcgcaa tctcgccgat gttgtccgcg ttcggcgtga
     1501 gcttcggcac cacgggcacg tccacggctg ctttcaccgc cgcggtgatc tcgcgcacca
     1561 gctccggatc ccggcccatc tccatgccgt accccttggc gtgggggcag gacaggttca
     1621 gctccaggcc gtcggagacc ggcgccagga tcttcgccac cgccacgaac tcctcgacgc
     1681 tgccgccgaa gatggaggtg agcagaaagc gatcttccgg gacgtcgagc gcggcgaaga
     1741 gctcggccga acgttcggcg cccgggttgg tcaggcccac ggcgttgacg aagcagccgg
     1801 gcgcgtactg gctgtagatg ggctcgcgat tgccggtgcg gggtgcaagc ccgatgctct
     1861 tggtggtcat cacaccgatc tgcggaacgt gcctgaacag gtactcgatg atcggcacgg
     1921 cggtcgcgac gatgcccgat gggatggtga acgggccgga tacgttcttg cccaggaatc
     1981 ggatcgtcaa ggcgggcggg tctcagcggt gtcgggattc ggagcgggat tctacaggtt
     2041 cggcgcgccc aatcgaacca cgcgtcaacc cgtagtgcct ccgctggcga tcagcacctc
     2101 gccggtgacg aaatcggact ccggaaagca gaacagggcg acggcgttcg ctgcgtcctc
     2161 cggagctccg gcccggcgca gcgggctcgt gctccggtag gcctcgatgg cttccggcgt
     2221 gaggcccacc cggtgctggc ggcccttcac atcgatggtt tccacctcgt cgccgatcgt
     2281 ctgggtgagg cgtgtctcga tgtacccgaa agccacggcg ttcacggtca cgttgtaggg
     2341 accccactcc ttgctcaagg acttggtcag gccgatcacg gccgccttgc cggcagcgta
     2401 gctcgcctgg gtggcggctc cgtacacccc cgatatcgag gagacgttca ccaccttgcg
     2461 acagggcggt ctttcgccgc gggcctgggc cgccttggcc tgctcgcgga agtggcggcc
     2521 cgcggcccgc agaatgcgga acggcgcggt ggcgtgcacg gcaagcatgg cctcccactg
     2581 ctcgtcgctg tggttgagca gcgcggagtt ccagatgtag ccggcgttgt tgaccaagac
     2641 gtccaggccg ccgaagccgt ccagagcggc atcgacgaga tcgtcgccga actcgggcag
     2701 ggtcacgtcc ccgggaaagg ccagcgcacg agggccagtg cccgagttga tctcgtcggt
     2761 gacggcgccg gccggctgct cgtccagatc attgaccacg acgtgggcac cttctgccgc
     2821 cagcttcaac gcgatggcgc ggccgatgcc gcggcccgca ccggtgacga tggcgacttt
     2881 cccctcgagt tttcccatga ttggaacggc ctccatggcg gttcgctcag acgctgcgca
     2941 gcggatcctc gccggaccag cccacggagg cttcccgaac gtggcggtag aaaccgacca
     3001 cgccgtcgga acgcgggtag atctcgatca tgtggccgag cagtttgcgc gcgtcgatga
     3061 aggcgaagtc cacgccgccg ctgcgcccca atccgcagcg ggtgaccacg ggcatgccgt
     3121 tgcgctcgaa gtactcgaag gcctcctcca tgtcgggaac catgatcgcc gtgtggtgga
     3181 ggccctcctc gtcgggcccg tacatgtccc ggtagggggt gttcggcgtg tcgttttcct
     3241 gctggaagaa ctcaaccatg agctcgcccc actgcccgta ggccgacgag tgcacgaaat
     3301 ccgtgggctc gccgcgatgc tcgccccagg tcagcgtgat gttctcgttg atgaaaaacg
     3361 gcccggcccc caccttctca gccatctccc gggccgcccg gtggatgtcg gtgacgtgat
     3421 aggcaacctg cacgatgtcg agtttgatgg tccttcctcc tcagtcctca aggaactccc
     3481 ggcgcacgtc gtccatggtg gcctcgctca cgccgatgct gcgaaggtac cccagcggtc
     3541 cgccccagcg cgcctcgagg ccgtccagga agtcgccgat ggcagtctcg ggcacccctg
     3601 cggcgtgcac catcgctgcc cgatccatgc cctccgggta gccgacggtg cgctcgatga
     3661 aatccgcctg ccggcccacg tcgcggttgg tgagaatgta gtctgcctcg atcagagcgc
     3721 ggtccacgcc cagcacggat aggagaaagg cggtggagac ccccgtgcgg tccttgcctg
     3781 cagtgcagtg cagcacgatg gggtgacggc cggcgtcggc gaatatctcg aacatcgcaa
     3841 gccacggctc gggcccgaac tccagatagc cgaggtagcg acgtccggag atgccggtgt
     3901 cccccaccag gcggctcaat gcatcgctgc cgccctccgg gatgacgggg atgtggtggt
     3961 aggcaacgcc catctcctcc aaaggacccc ggccctgctc tcggacctcg tcgggcttgc
     4021 gcaggtcgat ctgcgtggcg atgcccagat cgcgagccct ggcaacatcg gcttcggaca
     4081 tgcggtcctg gcgacccgcc cgatagtagc ggccccatcg caccgtgcga ccctcggtcg
     4141 tcgggtagcc gccgatgtcg cgaaagttga agcagccttc gaatcggtga tgccggtcgg
     4201 aatagtcatc gatcatatgt tgaagttcat tcggaaagcg cgtccatgtc aaacggaccg
     4261 tgtcgggacg accgtctcga tcagaccgtc tctgaccgac ccggcccgac gttgtcgtca
     4321 gtgagacagc ggcctggtga aaaagttgat ggtcccggcg tctggaacgt cgacgaagaa
     4381 cgtgccaccg gtcgccgaat gccggcaacg ttcaacagga aaaagtagtc gtgggcgtgc
     4441 tcgtgcttgt cgatccgggc gcctgcggtg gagatgcctg gcgcggctgc cttcggtgat
     4501 gagccaaagc ggtctggtca gtacatgggc cgcaggccct tgcccaccgg ctccctgccc
     4561 agcagcacca tgccggcctg gaggagcttt tccccggtaa ccggatcctg gtcgtgccag
     4621 cgggcgagga attcgttttc cttcagccac gtgcggtgaa acgaggcggg gtcctcgaag
     4681 tagagattct gacggtcgta gcccaccacc acgacgtagt gcccgtcgtc gaagttgcgt
     4741 cgccattcgg ccagcgagag gtagccgtcg gcccaggctt ggaccaggac gatgaccggg
     4801 cagttgcggt cgagatagtg cttcacgtca tccagcgacc agcccgttcc ggccttgacc
     4861 tcgaagcccc tggactcagc gaactcgatc atcctggcca gcggcgtgcc gagttcgggg
     4921 tcggcggcga gcgctttgag cagtttgtct tcgcgctcct cgacgccgta gtaggccagc
     4981 accatctgca gcgctttcac cccgcagtcg aaatcgaacg tctggcgttg gccatgcaga
     5041 tcgatgatct gcgccggcgc tgtgaaccgt cggacggtgt ccagcgttgt gcgcagggcc
     5101 tcggcaaaga cccggcgcag gtgatctacc gccagcacgt ccggcgtccc gtcggggtgc
     5161 atgtaaaggc cctgagaagt ttcctgaatc agcgcgggcg ccagctgccc ggctctgccg
     5221 ataccgttga ccgagtgggc ggcacagacg tgaccgtgaa cctgatagta ctccgaggcg
     5281 ttgacggtga cgtggacctc gggcagccgt ttctgcaggg cctcggcgta tgtctcggcg
     5341 tagatcgccg gcgcgtacag cttcggctcg ccgtcgcgct gcgtatgctg aaagttcatg
     5401 agatcgatct gatttgctgc cacgcccctg gcgaccacgg tggagtggcc gtccaggaga
     5461 aactcggcgc ccgcggagat gctcgcgctg atcttctgat gaaagccgcg caggtacttg
     5521 taggacaggt ggcggcgcag gtccaggctc ggctcacagc cgggccggta gacggctttg
     5581 ccgttgacgt cgcgcagggg tatcgactcg tccagcaact cggggtcgcg attggcctcg
     5641 agcaggaggc tgcagtacgg gaagacgatc tgctgattct cgagcaggtc ggtaaagtcg
     5701 tacaggcggt cggtgtgcca gtccacgttg gcaagcagtg ccggaaactc ttcggtgagg
     5761 ctatcactcg gtatctccgg cggaatgcgt atcccgctat gggggatgac caccagaaga
     5821 tctttcaggc tgcgcatgcg cgacccttga aatgtctgaa gaacgtcata gaaaagccgg
     5881 tccgcagaca ttgcgtggga ccggcgggga tccgcagtaa gcgccaaaaa tccggcaaat
     5941 acaatgggct cgcggcggcg tttgcctaca tgaaaaaaag tccggttgat gtgatgcacg
     6001 gctattgcat tcaaccccgt ttgccttaac ttacgcgccg ttcggcagct ccgaggggct
     6061 gtcgtttttc gttggaccgc cacaggagat ccaaggcgca tgaaaaagtc ccccccaccg
     6121 gtcgaagagg agcctccggg aggtgttcac ggccagaatc tagccctcag cctgcatcca
     6181 ccccattcga atacccgatc caacaagcca atcacctccc gtaccgcgcc agccgacgac
     6241 gcgcgtgtca tcaaagtcat ccctgcaaag tccaccgact cgacatccgt tgcatcgaag
     6301 gccgtcgagt ccgtgcagcg tcgggcgaag gtagtccggc tgcggaccgc agggcgcggc
     6361 aacccccgca cacgcgagtt tcggatccgc catttcccgg acgtttccga tgcggagtgg
     6421 aacgattggc gctggcagtc ccggcaccgc ttcaagaatc tcgcccagat cgagcgcgtg
     6481 ctcgaactgt ccgacgatga gcgcgcagcg ctcaccgttg gcggcatgat gctgcccgtg
     6541 gggatcacgc cctactacat gagtctgctc gaccccgttg atccgcagca tgccctgcgg
     6601 cggacggtca taccctcgca gaacgaattt cttcgcctgc cgggggaagc cgacgacccg
     6661 ctcggggagg acgcccacag tccggtgccg gggctcgtgc accgttaccc cgaccgggtg
     6721 ctgatgctgc caatcgattt ctgttccacc tactgccgct actgcactcg ctcgcgggta
     6781 gtaggccatg gggagctctc cccccagaca tcgcgcctgg aagccatgtt cgattacctg
     6841 cgaggcgcga cccaggttcg cgacgtgctc gtctctggcg gcgacgcgct tgccctcggc
     6901 gaggacaacc tcgactacat cctttccaac ctgcgggcca tagagcacat cgaattcgtc
     6961 cgcatcggca cccagatgcc ggccgtgctc ccgcaacgaa tcacccggga tctgaccagg
     7021 gtgcttcgca agtaccaccc gctctggatg agcctgcact tcctgcaccc ggaggaatgc
     7081 acgccggagt ccacccaggc ctgcgagcgg ctggccgatg ccggtatacc gctcggctcc
     7141 cagaccgttc tgctcaaggg cgtgaacgac gacgtgggcg tgatgacgcg cctcgtgcac
     7201 ggcctcctga agatgcgggt gcggccctac tacctgtacc agtgcgaccc gatcagtggt
     7261 tcggcccact tccgcacgac cgttgagaag ggcctggaga tcatcgaagg gctgcgcggc
     7321 cacaccacgg gttacgccgt gccgacctac gtcatcgatg cgcccggcgg tgggggcaag
     7381 attccggttc agccgaacta cgtgctcggc agagacgtcg acgagctcaa actccgcaat
     7441 ttcgagggac gggtcttcag ctatccggat ccccagcaac ctggcgccgg gaccaactga
     7501 acatgcgcat cggtatcacc tacgatctgc gttccgcgta cctcgccgcc ggctactcgg
     7561 aggaggaaac ggcggagttc gaccgcgacg acaccgtcga cgccatcgag gcggcgttgc
     7621 gccagctcgg gcacgagacg gtgcgcatcg gctgcgcccg cgagctcatc gagcaccttg
     7681 ccgcgggaca gcgttgggat ctggtgttca acatctgcga ggggctcagc ggtcccgggc
     7741 gggagagcca ggtgccagcc attctggagg tctacggcat acccgtgacg tttgccgata
     7801 cagccgtcat ggctctgagc ctgaacaagg cctgggccaa gacggtggtg gccgacgccg
     7861 gtgtggcgac cccgcagcat gccgtcatcg cccgggccgc tgacgttgcc acggtcgcgc
     7921 tcgagccgcc cctgttcgtc aagccgatcg gggagggcac cggcaagggc acaagcgccg
     7981 cttcggtcgt catgagcgcc gccgagcttg aacggcagag cctggccctg ctcgagcgct
     8041 accggcagcc cgtgctggtc gagacgtacc tccctggcag agagttcacc gtcggccttc
     8101 tgggcactgg cccgaatgcc agggtgctcg ggactctcga gatcgagctc ctggcaactg
     8161 ccgaggcgaa cgtctactcg tacctcaaca aggaggagtg cgagtcccgt gtgctgtacc
     8221 gactcgtcga tggtcgtgcc gatcccgtcg ttgcccgggc cgaggccgcg gcgctgggcg
     8281 cctggcgggc cctcggggcc cgggacggag ggcgcatcga ccttcgcgcg gactcggcgg
     8341 gggaacccaa tttcatcgag gcaaaccccc tggcagggct gcatcccgct cattccgatc
     8401 tgcccatgct ggctaccgcg gtgggaatgc cttatgcaga gctcattcgt gccatcgtcg
     8461 actcggcttc ggaacgtctt gccatggacg ctacggaaat gaaccgttgc gcgtagccat
     8521 cgcttacaac aagccgggag acgctgcggg cagcgcggaa gccgacgtgc tcgagcaggt
     8581 cgaagcggtt gagactgccc tcgtgggcgc cggtcaccag gtctgcgcgt tgccctgcac
     8641 gctggatctc gccggattcg atggtgccct ggcaaagcag cggcctgacg tcgtcttcaa
     8701 tctggtcgag gccctgggcg gaaccgaccg cctggccgtg ctggtgacgc tgctcctcga
     8761 cgcccgcggt gtcccctaca ccggagcggg atcgcgggcg ctggagctca gcgccgataa
     8821 ggtcggcgcc aaggaacgaa tgcgcaggct cggcctgccg acgccggact ggtacgtctg
     8881 tgagaagacc caggtggatt tccctggggc agggtgctac atcgtcaagg cacgctttga
     8941 gcacgcctcc cttggcctgg acgaccagtc ggtcgttcag gttgctgatg ccgggcagct
     9001 caccaggcgc atccgcgcac gcggaaagca cagtggcgcg cagcagttcg ccgaacgcta
     9061 cgttgccggg cgtgagttca acgtggcgct tctggcttcg gaaaccggcc ccgcccaggt
     9121 gctgcctccg gcggagatcg acttctcggc gtttgcgccg ggcaaacccc gcatcgtcgg
     9181 gcatgacgcc aagtggtcga gccgcgcctt cgagtatcat gcgaccccgc ggcgtttcga
     9241 cttccccgca gccgatgccc ggttgctcga cctgcttgga accctcggga tgcaggtctg
     9301 ggagtgcttc gcgatgcgcg gctgcgcccg cgtcgacttc cgggtcgacg aggacggccg
     9361 accctggatt ctggagatca acccgaatcc gtgtctctcg cccgatgccg gcttcgctgc
     9421 cgcgctcgat gcggccggga tcccggcgga ggagatggtc gggcgactgg tcaggacggc
     9481 cgctacggac tgagcatgac ctcaccctgc aaagagcatc cccccgggga cgcctgggtt
     9541 catctgcggg agccggcggc acgcgaccgt ggacccgtgg cctcggtgct ggtctcgacg
     9601 ggatttttca gtgatgccga agtcgctgtg gcgttggaag tcttcgatgc ggcattgcag
     9661 ggtaaggact ccggctacga gcatctcgtg gccgaatccg ccggaagggt gatcgggtac
     9721 gtctgctggg gcgggcccat agagatgacg ctctcgagct tcgaccttta ctggatcgcc
     9781 gtggatgcgg cggcccgggg ccgcggggtc ggtcatcggc tgctggccgc ggccgagagc
     9841 ctggcggcgg cgtcgggctg tgcccagctt tttgcggaga cctccggacg tgccgattac
     9901 gcgcccacgc acagcttcta cgaatcctgc ggctacctgc gcgcggccga gctcgcggac
     9961 ttctacgcgc caggtgacgc caaagttttc tacgtcaaac ggctgaccgg cggcaatcag
    10021 gccccaccgg aatgacggcg ttccgaccgt gttccgagcg cggccctctc tagcggggcg
    10081 cgtctgcgac cagcgcctcc gtcgacatca tcatgccggc cacggacgct gcgttgtgca
    10141 gcgccgactt tacgaccttg caggggtcca gtatgcccat ttccagcatg tcgccgaacg
    10201 tttcggtctg cgcgttgaac ccgaagtgtc cctgctccga ctgaatcttt ctcagcacca
    10261 cgcctggctg cagaccggcg ttggcgacga tctggcgcag gggcgcctcg gcagcccgcc
    10321 gaagcgcgtc cacgccgctt gcctggtcct ggtttcgggg cgcggccttt gcgagctcgg
    10381 ctgaggcgcg cagcagtgcc acgccgcctc ccggcacgac gccctcttcc acggcagcgc
    10441 gagtagcagc gagcgcgtcg tccacgcgca tcttcctctc cttcatggcg acttccgtgg
    10501 cgccgccgac gttgatcacc gccacgccgc ctgccagttt ggcaagacgc tcctgcagct
    10561 tttcgcggtc gtactcggac ttggactcct cgatctcgcg acggatctgc gagatgcgcc
    10621 cggcgatagc gtcggtcccg ccgcgacccc cgatgagcgt cgtgtcccct ttggtcagca
    10681 atacccgttg cgcctcgccg agatcctcca gcgccacctt ttccaggctg aatcccgcgt
    10741 cgctggagat gacctttccg ccggtgagga tcgccaggtc ctgcagcatg gccttgcgcc
    10801 ggtcacc
//
LOCUS       DBNHKV010000476         5181 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_563744, whole genome shotgun sequence.
ACCESSION   DBNHKV010000476 DBNHKV010000000
VERSION     DBNHKV010000476.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5181)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_563744"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="AC16PP_15545"
     CDS             <1..512
                     /locus_tag="AC16PP_15545"
                     /inference="COORDINATES: protein motif:HMM:NF024802.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6483095.1"
                     /translation="DEMTQGRVYAGLSPEQKAQGVALTRLVEDHLYWIGVASRWLDDA
                     WWPHVVENFFGFVPALIRPFAAGGARKQVRQTYHLQGLGRHTLEEQRGFAKRDLQALE
                     DAVPRTGFLFGDAPGIFDFTVAGMMAGFYDNQPPTWLTELALTYPGLNEYTERVQEAI
                     GVYGRHVDA"
     gene            complement(535..1920)
                     /locus_tag="AC16PP_15550"
     CDS             complement(535..1920)
                     /locus_tag="AC16PP_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450 family protein"
                     /protein_id="HIR6483096.1"
                     /translation="MSSESEFVKASKGDPVRILGKSRPSLSRSGMPLFARNGTAADYR
                     TYEIYQRERVHESTEKIRPIELVSSEFVRDPYPVLGVLRENYPCYRDWLGNAYWLTRY
                     NQVTSVFTDDANFETRPKLWFYRMQGYGRDLRSELPMQIAHERLIDANAVPVAEEIVS
                     AFAARGSADLALEFAARLPMELLAKVLDLPAEDFHWFVERYWRMQRGFDWNAADEQAG
                     KRAMTELADYFRPLLEARRADPGDDMVSAIATLELEDGPTTAEDVVVTLLEADHETMH
                     GALANFWFLLLTHPDQLEQVAQERRLLKFAYLEALRHSTPVLWKKRFALHEVERFGRL
                     LPIGGLFYCAAAAANRDPWIFSDPDEFDITRRDLCQREPRGHYRSDGLAAGVALGLGR
                     PSPYPGIPEDRPRSLYAMTRDTIVTASNILLDTLPDIRLQTQAQPALRCLRLGEMHTC
                     WHLPAEFTPRS"
     gene            complement(1924..3375)
                     /locus_tag="AC16PP_15555"
     CDS             complement(1924..3375)
                     /locus_tag="AC16PP_15555"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6483097.1"
                     /translation="MSTESAFADTLRRTIGAETIAGDADPGAKIDFPLMPLGEAADMG
                     RAEDGTPLIDPRIFYSAEFRRNPWPYYRILRDHYPVYFNKLNNTYYVTRYADMTECYY
                     DDIGFNTIPKGSSNGVLGNVTHEFSGVEHRRRRNLFGQHLVGKSLEHRVPAIERLALE
                     MIDDFANLDESVRQVSGKVATIELGRAFAYEFPVRVVCDVLGFPKEAQAHFFYWYDSI
                     MSGLGASETHKQGMEARQHLEDYVSELVEERRRSPGYLYDNQGNKLGPDIISKLCASK
                     IDGDYLSSVEITSYIALVVAGGGETTRGTIMNMWYSLLQHPEQFAAVVADDALWDAAF
                     HETLRHSNPIGGQPRHATFDVELHGVHIPAGSLIEMVNFSGSHDERVFKDPETFDIFR
                     DDLYSGKLLRSGYSKDGVCSHMAFGVGPHMCPGAWISHQEATIGSRLLAKRLKNARIN
                     RERMPKDIDGVSLAPIGINKLRDLWIDYDIDQP"
     gene            3690..4244
                     /locus_tag="AC16PP_15560"
     CDS             3690..4244
                     /locus_tag="AC16PP_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6483098.1"
                     /translation="MKVDLNATVQLLGNLGEFVAAIAVVASLLFVGFQVRQSDRTQRT
                     TSLQSVLDGHRDRTFAPLYESEDMADLWARGLTSFDDLSETEKRRFFSFMFEQCFQMQ
                     QVMQLHEQGLVPDVDFDAWLYYTGVLFNCPGGRAVWPIIERLVTPTVRDLMNEYWDEN
                     PTLPTYQELNPLFVADRAAGVIGT"
     gene            4362..>5181
                     /locus_tag="AC16PP_15565"
     CDS             4362..>5181
                     /locus_tag="AC16PP_15565"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6483099.1"
                     /translation="MPSIPSNIEEVTNEWMSEILGAEVTGFDVTFLEGGVLSDAFKVH
                     DIAYGAPAGDQPASVVVKLTNAVPERRAVAAQNKAYVKEVRFFKELADQVPLKTPEVY
                     ALLDDGSETFEYFCIVMEDLTTHSEVFDQVIDPPNEAWTRKINLEAAGLHAAFWESDV
                     LELDWLRMPDRQFRHTMHDTARGCPEAVDTFVSLWEKAFGQSPFQDAWASVKPLTEII
                     TGPRCEQILEAINRRYSSRPWTLVHNDLRADNIFRTRGSDAATGELTYIDWQLTS"
BASE COUNT          968 a         1508 c         1669 g         1036 t
ORIGIN      
        1 tggacgagat gacccagggc cgcgtctatg cggggctgtc ccccgagcag aaagcgcaag
       61 gggtggcgct gacccgtctg gtggaggacc acctctactg gatcggggtg gcgagccgct
      121 ggctggacga cgcctggtgg cctcacgtgg ttgagaattt ctttggattc gtgccggcgc
      181 tcatccggcc tttcgctgcc ggaggcgcgc gcaagcaggt tcgccagacc tatcatcttc
      241 agggtctcgg tcggcacacg ctggaagagc agcgcggctt tgcgaagcgg gacctgcagg
      301 ccctcgagga tgccgtgcct cggaccggtt ttctcttcgg cgatgcgccc gggatattcg
      361 acttcacggt ggccggaatg atggccggct tctatgacaa ccagccgccc acctggctca
      421 cggagctggc cctgacctat cctgggctca acgaatatac cgagcgggta caggaggcca
      481 tcggcgtgta tggccgccat gtggatgcct gagcgggtcg ggtgagggcg ggaatcagga
      541 tcgcggggtg aactcagcgg gcaggtgcca gcaggtgtgc atctctccca ggcgcaggca
      601 ccgcagcgca ggctgagcct gggtttgcag acgaatgtcc ggcagggtgt ccagcagaat
      661 gttcgaagcg gtcacgatgg tgtcgcgggt catggcgtag agcgagcggg gtcggtcctc
      721 gggaatgcct gggtacggcg acggtctgcc gagccccaat gccactcctg cagccagacc
      781 gtcgcttcgg taatgcccgc gaggttcccg ctggcagagg tctctgcgcg tgatgtcgaa
      841 ctcatcggga tcggaaaata tccacggatc ccgattggca gcggcggcgg cacagtaaaa
      901 caagccaccg atgggcagca gccttccaaa cctttccacc tcgtgaagcg cgaagcgctt
      961 cttccacagc acgggggtgg agtggcgcag ggcctcgagg taggcgaact tcagcagccg
     1021 acgttcctgt gcaacctgtt cgagctggtc ggggtgggtg aggagcagaa accagaagtt
     1081 ggcaagcgcc ccgtgcatgg tctcgtggtc ggcttccagg agagtcacca cgacgtcttc
     1141 tgctgtagta gggccgtctt cgagctccag ggtagcgatc gccgagacca tgtcatcccc
     1201 cggatcggca cggcgcgcct cgagtaaggg cctgaagtag tctgccagct cggtcatcgc
     1261 ccgcttgccc gcctgctcgt ctgcggcgtt ccagtcgaat ccgcgctgca tgcgccagta
     1321 gcgctccacg aaccagtgaa agtcctccgc cggaagatcc agcacttttg ccagcagttc
     1381 catgggcagg cgggcggcga actccagagc aagatccgcg gagcccctgg ctgcaaaagc
     1441 agacacgatc tcctcggcta ccggcactgc gttggcgtcg atgagccgct cgtgcgcgat
     1501 ctgcatgggc agctcagaac gcaggtcgcg tccgtagccc tgcatacgat agaaccatag
     1561 cttcggacgg gtttcgaagt tggcatcgtc ggtaaagacg gacgtcacct ggttgtagcg
     1621 cgtaagccag taggcgttgc ccagccagtc ccgatagcag ggatagtttt cccgaagcac
     1681 gccgagaacg gggtaggggt cccggacgaa ctctgacgag acgagctcga tgggacgaat
     1741 cttctcggtg gattcgtgca cacgctcgcg ctgatagatc tcgtaggtac ggtaatcggc
     1801 agccgtgccg ttgcgcgcaa acagcggcat gccgctgcgc gacaggctcg gccgcgactt
     1861 gcccaggatc ctcacaggat cccctttgct tgctttgacg aactcggact cgctgctcat
     1921 ggcttagggc tggtcgatgt cgtagtcgat ccacagatcg cgcagtttgt tgattccgat
     1981 cggcgcgagg ctcacgccgt ctatgtcctt cggcatcctc tcgcggttga tgcgagcgtt
     2041 cttcagacgc tttgccagca ggcgcgatcc gatggtcgcc tcctgatggc tgatccaggc
     2101 gccaggacac atgtgtggcc ccacgccgaa ggccatgtgg ctgcagacgc cgtctttgct
     2161 gtagccgctg cgcagcagct tcccggagta cagatcgtcg cggaagatat cgaaagtttc
     2221 cggatccttg aacacccgct cgtcgtggct gcccgagaag ttcaccatct cgatgagcga
     2281 gcccgcaggg atgtgcacgc cgtgcagctc cacgtcgaag gtggcgtggc gcggttgccc
     2341 gccgatcggg ttggaatggc gcagggtttc gtggaaggcg gcgtcccaga gagcgtcgtc
     2401 ggcaactacg gcggcgaact gctcgggatg ctgcagcagc gaataccaca tgttcatgat
     2461 ggtgccgcgg gtggtctcgc ccccgccggc caccaccagg gcgatatacg aggtgatctc
     2521 cacggagctg aggtagtcgc cgtctatctt gctcgcgcaa agcttggaaa tgatgtccgg
     2581 acccagtttg ttgccctggt tgtcgtaaag atatccgggg ctcctgcgtc gctcctccac
     2641 gagctcggac acgtagtcct ccaggtgctg gcgggcctcc atgccctgct tgtgggtctc
     2701 agacgccccg agtccggaca tgatggagtc gtaccagtag aagaagtggg cctgcgcctc
     2761 cttgggaaac cctagcacat cgcacaccac tcggacggga aattcgtagg caaaagcgcg
     2821 acccaactcg atggtggcaa ccttgccgct cacctggcgg acgctttcgt cgagattcgc
     2881 gaaatcgtcg atcatctcga gcgccagccg ctcgattgcg ggcacccgat gttccaggga
     2941 tttccccacc aggtgctgcc cgaacagatt gcgccggcga cggtgctcga cgccgctgaa
     3001 ctcgtgggtg acgttgccca gcacgccgtt ggatgagccc tttggaatgg tgttgaagcc
     3061 gatgtcgtcg tagtagcact cggtcatgtc cgcgtatcgc gtcacgtagt acgtattgtt
     3121 cagcttgttg aagtacaccg ggtagtggtc gcgcaggatc cggtagtagg gccaggggtt
     3181 tcgacggaac tctgccgagt agaagatgcg cggatcgatc aggggggtgc catcctcagc
     3241 gcgccccatg tcggcggcct ctcccagcgg catcagggga aagtcgatct tggcgcccgg
     3301 gtcggcgtcg ccggcaatcg tctcggcgcc gatggtgcgg cgaagagtgt cggcaaatgc
     3361 gctctcagtg gacatgtgct ctgtatcctt tcatcccgac cctgatccta gggctttttt
     3421 ccgggtttct ccaagcacaa actgtttgtg cgtcgtttcg attggcgttc tccagaaagg
     3481 ggtgaccaga ccgattcggc gagcgctcag ggcttaaagg gctttgctga ccgcatcgga
     3541 ggccattttt ccggttcgac cacggcgttg gttgcaggca acaggacaga aaacacggct
     3601 cgatacccga gggcgtcgct cattactcat caacggctga cgacaaactg caagattagg
     3661 ataggctttc gggaatcaac gggagggaca tgaaagtgga tctgaacgct actgtgcagc
     3721 tattggggaa tctcggggag tttgtcgcgg cgatcgccgt ggtggcgtcc ctgctgttcg
     3781 ttggcttcca ggtcagacaa agtgaccgca cgcaacgcac gaccagcctg cagtcggtgc
     3841 tcgatggaca ccgggatcgc acgtttgcgc cgttatacga gagcgaggac atggcagatc
     3901 tgtgggctcg gggtctcacg tcgttcgacg acctgagcga aaccgagaag cgacgctttt
     3961 tcagcttcat gttcgagcaa tgctttcaga tgcagcaggt aatgcagttg catgaacagg
     4021 gtctggtccc cgacgtcgat ttcgatgcgt ggctttacta cacgggcgtg ctgttcaatt
     4081 gtcccggggg tagggcggtg tggccgatca tagagaggtt ggtcactccg acggtccggg
     4141 acctgatgaa cgaatattgg gatgagaacc cgacgttgcc tacgtaccag gagctcaatc
     4201 cgctcttcgt cgcggaccgg gcagccgggg tgataggcac atagagccgc gaagcgtcct
     4261 tccattcctt gtgcgcgcgg gctgtttcca gttggctgcg cagaaagtat cgttgacgga
     4321 tcgtgatctt tcctcaatcg gccggctggt gagctatcgt tatgccttca atcccctcga
     4381 acatcgaaga agttaccaac gaatggatgt cggagatcct cggtgcggag gtcaccgggt
     4441 tcgatgtgac attccttgaa ggtggcgtgc tgtctgatgc gttcaaggtt cacgacatcg
     4501 cctacggtgc gcctgcggga gatcagcctg cgtcggtcgt cgtgaagctg accaatgcgg
     4561 tcccggaacg gcgtgcggtc gccgcgcaga acaaggctta cgtcaaggag gttcgcttct
     4621 tcaaggagtt ggcagatcag gtccccctga agacgcctga agtttacgcg ctgctcgacg
     4681 acggcagcga aaccttcgag tatttctgca ttgtcatgga agacctgacc acgcactcgg
     4741 aggtattcga tcaggtgatc gatccgccga acgaggcctg gacgcgcaag atcaatctgg
     4801 aagccgcagg cctgcacgcg gctttctggg aatcggacgt cctcgagctg gactggttga
     4861 ggatgcccga ccggcagttc agacacacca tgcatgacac cgcgcggggc tgtcccgagg
     4921 ccgtagacac ctttgtcagc ctctgggaga aagcattcgg acagagcccc ttccaggacg
     4981 cctgggcctc ggtaaaaccg ctgaccgaga tcatcaccgg accccgctgc gagcagatcc
     5041 tggaagccat caatcgtcgc tacagcagcc ggccctggac gctggtgcac aatgatctgc
     5101 gcgccgacaa catatttcgt acgcggggtt cggatgcggc cacgggcgag ctcacataca
     5161 tcgactggca gttgacgtca c
//
LOCUS       DBNHKV010000477         8762 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_493780, whole genome shotgun sequence.
ACCESSION   DBNHKV010000477 DBNHKV010000000
VERSION     DBNHKV010000477.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8762)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8762
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_493780"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1735)
                     /gene="mrdA"
                     /locus_tag="AC16PP_15570"
     CDS             complement(<1..1735)
                     /gene="mrdA"
                     /locus_tag="AC16PP_15570"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR6483100.1"
                     /translation="MPPVLALKDSSSERRSFSARAAVLFALATCLVSVLILRLLQLQV
                     WEYDTYRTRSEENRIQVQPLAPPRGLIFDRNGVLLADNRPVHSLALVRERIADLDALI
                     TELGSLVSISEAEIESLRGRLLRRRRPYEPVALKLTLSEEDIAVLAVNRHRLVGVEIS
                     TQLVRYYPFGPLLAHAVGSVRRVTEDDLRELDPVRYSATKFVGRRGVERFYERSLHGE
                     VGYQRVETDAHGRIRQVLEINEPAAGQNITLQLDSRLQIAASAALGDRRGAIVALDPR
                     SGGVLALVSHPQYDPNQFVTGMTSAQFRELTSSRDTPLFNRAINGQYAPGSTFKPIVG
                     LAGVASGVTRWADQIDDQGWYQLPGQERIYRDWSWAVDDSGGQGLTNLTRAIYRSSNV
                     YFYDLATRLDIDRIATFAAQFGYGQRTAVDIPDASKGLLPTRVWKKGAKGEIWYPGDN
                     VNIGIGQGDLLATPLQLATVAAVVANRGEFVRPQMLLSSDRPLLEFDPARPMPRVEGP
                     SAEDWERMVDAMEDVVHRGNRGVGQNGTAWAYIGRNIPYRMAGKSGTAQVVEIKQGEE
                     YDEEELEEYQRK"
     gene            complement(1737..2204)
                     /gene="rlmH"
                     /locus_tag="AC16PP_15575"
     CDS             complement(1737..2204)
                     /gene="rlmH"
                     /locus_tag="AC16PP_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR6483101.1"
                     /translation="MKIRVIAVGARPRPWVADGFREYARRLPREMPLSLVEVAPGGGR
                     GRSAEQVVAMESERLLSHLGPRDWVVALDVGGEGCSTRELALSLESWRMQGRDVAFLI
                     GGAEGLAPTCLARADMALSLSELTFPHEMVRVILAEQIYRAWTILSGHPYHRD"
     gene            complement(2224..2571)
                     /gene="rsfS"
                     /locus_tag="AC16PP_15580"
     CDS             complement(2224..2571)
                     /gene="rsfS"
                     /locus_tag="AC16PP_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR6483102.1"
                     /translation="MTAEALRDLVVDALEERKGRDVVAMDVTKSTDITDFMIIASGTS
                     NRHVKALVDQVVESAKAGGNPPLGVEGRETHEWVLVDLGDVLVHVMQADTRAFYDLER
                     LWSDIPLDSETLN"
     gene            complement(2561..3193)
                     /gene="nadD"
                     /locus_tag="AC16PP_15585"
     CDS             complement(2561..3193)
                     /gene="nadD"
                     /locus_tag="AC16PP_15585"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR6483103.1"
                     /translation="MIALFGGTFDPVHRGHLHGAATVCEALGLEAISMVLSARPGHRA
                     SPGASAEHRWEMLCLACADDGRLVPDDRELHRDRPSYTVETLLEVRGEIGDESLVWVL
                     GSDAYADLTSWHRWGEVLDLTNLVVLERPGGPSRWAPPLDGISRRHRVEGMLDAPWGQ
                     ILFLKAPMQEVSASEIRAAIAGGRPVAHLLPERVATYISRHGLYGAPSDS"
     gene            complement(3190..4452)
                     /locus_tag="AC16PP_15590"
     CDS             complement(3190..4452)
                     /locus_tag="AC16PP_15590"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR6483104.1"
                     /translation="MTDIAAYMGEVARSARAASRVIAAASSDSKSRALKAMVEALDED
                     RETLKRANEVDVRAAGDKGVDQPLIDRLVLDDRAIDRMLEGVLQVDKLADPVGEITDL
                     AYRPTGIQVGRMRVPLGVIGIIYESRPNVTADAASLCLKSGNACILRGGSEAIESNRA
                     IAAGIARGLAAAGLPETVVQLVDTTDRDAVGELLKADRFVDVIVPRGGKGLIERIIRE
                     SRIPVIKHLDGVCHVYIDDAADLEMAAAIAVNSKIEKYAVCNAMETLLVANGVADRVL
                     PVLGDRFRQAGVELRGCERARAAFSDMKPATEEDWREEYLGPVLAVRVVEGIDEAIAH
                     INTYGSQHTDAIVSNDYGRTRRFLAEVDSASVMVNASTQFADGFEFGLGAEIGISTDK
                     LHARGPVGLEGLTSQKFVVFGGGEIRHR"
     gene            complement(4520..5308)
                     /locus_tag="AC16PP_15595"
     CDS             complement(4520..5308)
                     /locus_tag="AC16PP_15595"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR6483105.1"
                     /translation="MDWLQIIILALVQGITEFLPISSSAHLILPALLTEWPDQGLAFD
                     IAVHAGTLLAVLSYFRREIGRFAASGARLLARGQRDDDADLLLKLIAATLPIVLVGYL
                     AHDWVESSLRTVPVIASATIVFALALWWADGRRSGADHPSWTAAMLIGAAQVIAIVPG
                     TSRSGITMTAALLLGLSRTGAARFSFLLSIPTILGAAVLEAASLGAGEHAVRWAELVA
                     GAVLAGAGAYCCIHFFVKLVERTGMLPYVVYRLMLGTLLLGLAL"
     gene            5388..5981
                     /locus_tag="AC16PP_15600"
     CDS             5388..5981
                     /locus_tag="AC16PP_15600"
                     /inference="COORDINATES: protein motif:HMM:NF014269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR6483106.1"
                     /translation="MEIPLFPLRVVLFDGGRLPLQIFETRYLDMVSRCTRESIPFGVV
                     LIRGAGSDARLDPLANQPAIFEVGTEAHIVDFNQLPNGRLGIIVRGGRKFRVVSTREQ
                     ADHLLLGTVDFLPAEPESTVPSEHAPLVDILKELVKHPGVQKLGLEIDFADARSVGAR
                     LAELLPIEPEIKQSLLQLQLPRERLQELTRLVNKFRD"
     gene            complement(5978..6421)
                     /gene="ssb"
                     /locus_tag="AC16PP_15605"
     CDS             complement(5978..6421)
                     /gene="ssb"
                     /locus_tag="AC16PP_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR6483107.1"
                     /translation="MARGINKVILIGNLGRDPETRYSQGGNAVTSFSVATTENWRDRQ
                     SGEQQERTEWHNVVCFARLAEIAGEYLRKGSKVYIEGSLRTSSWEQDGQKRYRTEVMA
                     RDLQMLDSRGGGQGGQGGGFESAAPAASENAPTLADDDFDDDIPF"
     gene            6631..>8762
                     /gene="uvrA"
                     /locus_tag="AC16PP_15610"
     CDS             6631..>8762
                     /gene="uvrA"
                     /locus_tag="AC16PP_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR6483108.1"
                     /translation="MDSIAVRGARTHNLKNVNLDIPRDRLVVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSMMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIYDYLRLLYARVGEPRCPNHDLPLDAQTVSQMVDQVLGLPEDKRVMVLAPVVTERKG
                     EHLHVFQELMANGFIRARIDGIVVDLDHPPNLEKNKKHTVEAVIDRLRIRADAQQRLA
                     ESFETALDLAEGIAKVASMDDADEPELVFSARFACPACGYSISELEPRMFSFNNPAGA
                     CQACDGLGVKQFFDPGLVVQDEELTLAEGAIRGWDRRNVYYFHMLSSLAAHYGFDVET
                     PFRKLPKKHRDAILYGSGRERIDFSYANDRGDVIHRRHRFEGVIPNMERRYHETDSSL
                     VRENLQKYLRVRSCPDCGGTRLREASRNVFIDGVSLPEITSGSVEGSLAYFDELKLSG
                     RRGEIADKILKEIRARLRFLVDVGLNYLTLDRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYILDEPSIGLHQRDNARLLKTLEHLRDLGNTVLVVEHDEEAIRSADHIIDIGPGAGV
                     HGGCVVAAGSLEKILKSRRSITGQYLSGRKKIPVPEKRTAFVEEETIRIIGATGNNLQ
                     SVSVEIPCGLLTCVTGVSGSGKSTLINHTLYPVAATRLNKATTLTPAPVEEVLGLNHV
                     DKVVDIDQSPIGRTPRSNPATYTGLFTP"
BASE COUNT         1611 a         2965 c         2699 g         1487 t
ORIGIN      
        1 gtttgcgctg gtactcctcg agttcctcct cgtcgtactc ttcgccctgc ttgatctcaa
       61 ccacctgggc ggtgccggac ttgccggcca ttcgatacgg aatgttccga ccgatgtaag
      121 cccaggcggt cccgttctga cccaccccgc gattgccccg gtgcaccacg tcttccatgg
      181 catcgaccat gcgctcccaa tcctcggcgc tcggcccttc tacccggggc atcggtctgg
      241 ccggatcgaa ctccaggagc ggcctgtcgc tggaaagcag catctgaggg cgaacaaact
      301 ctccgcggtt ggccaccacg gccgccacgg tggcgagctg gagcggggtg gcgagcaggt
      361 cgccctgacc gattccgatg ttgacgttgt cacccggata ccagatctcg cccttcgcgc
      421 ctttcttcca tacccgcgtt ggcagcaggc ccttgctcgc atcgggaatg tcgaccgcgg
      481 tgcgctgccc gtagccgaac tgggcggcga aggtggcgat ccggtcgatg tccagccggg
      541 tggcaagatc gtagaagtag acgttggaag accggtaaat ggctcgcgtc aggttggtga
      601 ggccctgacc tcccgagtcg tctaccgccc agctccagtc ccggtatatg cgctcctgcc
      661 cgggcagctg gtaccacccc tgatcgtcga tctgatccgc ccacctcgtc acgccgctcg
      721 cgacccccgc gagccccaca atgggtttga aggtcgagcc cggcgcgtac tggccgttga
      781 tcgcacgatt gaacagcggg gtgtcccgag aggacgtcag ctcccgaaac tgggccgacg
      841 tcatgccggt cacgaactgg ttggggtcgt actgcgggtg actcaccagg gccagcacgc
      901 caccgctgcg ggggtccaag gccacgatgg cgccgcgccg atctcccagg gcagccgacg
      961 cggcaatctg caggcggctg tccagctgaa gcgtgatgtt ctgaccggcc gcaggctcat
     1021 tgatctccag cacctggcgg atccgaccgt gggcatcggt ctccaccctc tgatagccca
     1081 cctcgccgtg cagcgagcgt tcgtaaaagc gctccacccc tctgcgcccg acaaatttag
     1141 tggcgctgta gcgaacgggg tctagctcgc gcagatcgtc ctcggtgact cgtcgaaccg
     1201 agcccacagc gtgcgcaaga agcggaccaa aggggtagta gcggacgagt tgggtggaga
     1261 tctcaacgcc gacgagccgg tggcggttta cggcaagcac ggcgatgtct tcctcgctga
     1321 gcgtcagctt gagcgccacc ggctcgtagg gccgtcggcg tcgcagcagg cggccgcgga
     1381 ggctttcgat ctccgcctcg ctgatgctga ccagcgagcc cagctcggtg atgagggcat
     1441 cgaggtcggc tattcgttcg cgcaccagag caaggctgtg aacgggacga ttgtcggcca
     1501 gcagcacgcc gttgcgatca aaaattagcc ctcgtggagg cgccagcggc tgaacctgta
     1561 tgcgattctc ttccgaccgg gtccggtacg tgtcgtactc ccacacctgc agctgcagca
     1621 gacggagtat caggacggat acgaggcagg tagcgagcgc gaacagcacc gcggcccggg
     1681 cagagaagga ccggcgttcg gacgagctgt ctttgagtgc gagaacaggc ggcatgtcag
     1741 tcccgatgat aggggtggcc ggagagaatg gtccaggccc ggtagatctg ctccgccagg
     1801 attaccctca ccatctcgtg ggggaaagtc agctcggaca gcgacagcgc catgtcagca
     1861 cgagccaggc acgtgggcgc gagcccttcg gcgcccccaa tgaggaaagc gacgtcccgc
     1921 ccctgcatcc gccagctctc caggctcagc gccagttccc gggtgctgca accctcacct
     1981 cctacatcca gggcgacgac ccagtctctt ggacccaggt ggctgagtaa tcgttccgat
     2041 tccatcgcca cgacctgctc ggcagaccgg ccacggcccc cgccgggcgc cacctccaca
     2101 agagacagcg gcatttcccg cggcaaccgc ctggcgtact cgcgaaaccc gtcggcaacc
     2161 cagggccgag gtcgtgcgcc cacggcgatg acccggatct tcatggcagc cttcccgggc
     2221 gcctcagttg agcgtttcgg agtccaacgg tatgtcggac cagagccgct cgaggtcgta
     2281 gaatgcccga gtgtcggcct gcatcacgtg cacgagcacg tcaccgagat ccaccagcac
     2341 ccactcgtgg gtttcacggc cctccacgcc gagcggcggg ttaccgccgg ctttcgccga
     2401 ctccaccacc tgatcgacaa gtgccttcac atgccgattg gaggtgccgc tggcgatgat
     2461 catgaaatcc gtgatgtcgg tactcttggt cacgtccatc gccacgacgt cgcggccttt
     2521 ccgttcttcc aacgcatcca cgaccagatc gcgcagggcc tcagctgtca ctcggtgctc
     2581 cgtatagtcc gtgccgacta atatatgttg ccactcgctc cgggagcaaa tgcgccacgg
     2641 ggcgcccgcc ggcgatggct gcgcgaattt ccgaggcgga cacctcctgc atgggagctt
     2701 tcaggaagag aatctgcccc cacggcgcgt ctagcatccc ctcgacccga tgacgacggc
     2761 tgattccatc caggggcggg gcccagcggc tcggcccgcc cggccgctcc agcaccacga
     2821 ggttggtcag atccagaact tcaccccagc gatgccagct ggtcagatct gcgtaggcgt
     2881 cgctgccgag cacccagacc agggactcgt caccgatttc tccgcggacc tccagcagcg
     2941 tctccaccgt gtaggacggc cggtcacgat gaagctcgcg atcatccgga accaggcgcc
     3001 catcgtcggc gcacgccagg cacaacatct cccagcggtg ctcggcggac gctcccggcg
     3061 aagcgcggtg acccggccgg gcggacagca ccatggagat ggcttcgagg ccgagcgcct
     3121 cgcagaccgt ggccgcgccg tgcagatgtc cccggtgaac cggatcgaag gtgccgccaa
     3181 acagagcaat caacggtgcc ggatctctcc gccgccgaag accacgaact tctgcgacgt
     3241 gaggccttcg aggcccaccg ggccacgggc atggagcttg tccgtcgaga tgccgatctc
     3301 ggctcccagg ccgaattcga agccatctgc aaactgggtc gaggcgttga ccatgaccga
     3361 cgccgagtcc acctccgcaa gaaatcgacg ggttctgcca tagtcgttgg agacgatggc
     3421 gtccgtatgc tgggacccgt aggtgttgat gtgagcgatc gcctcgtcga tcccctccac
     3481 caccctcacc gccagcaccg gcccgagata ctcctcccgc cagtcttcct cggttgccgg
     3541 tttcatgtcc gaaaaggcgg cccgagcccg ctcgcagcct cgcaattcca cgcccgcctg
     3601 gcgaaaacgg tcgccgagca cgggcaggac ccgatcggct acgccgttgg ccaccagcag
     3661 cgtctccatg gcgttgcaga ccgcgtactt ctcgatcttg gagttgacgg cgatggcggc
     3721 cgccatctcc aggtcagcgg cgtcgtcgat gtagacgtgg cacacgccgt cgaggtgctt
     3781 gatgaccggg attcgtgatt cccggatgat gcgctcgatc aggcccttgc ctccccgagg
     3841 cacgatgacg tccacgaacc gatccgcctt gagcagctct cccaccgcat cgcgatccgt
     3901 cgtgtcgacg agctggacga ccgtctccgg cagtcctgcc gcggccaggc cacgggcaat
     3961 tcccgcggcg atggctcggt tcgactcgat ggcttccgaa ccgccccgaa ggatgcaggc
     4021 gttgcccgac ttcaaacaca ggctggcagc gtccgcggtc acgttcgggc gcgattcgta
     4081 gatgatcccg atcacaccca ggggcacgcg catccgcccc acctgaatgc ccgtcggacg
     4141 gtaggccaga tcggtgatct cccccaccgg atcggccagc ttgtccacct ggaggacgcc
     4201 ttccagcatt cggtcgatgg cacgatcgtc gagaaccaga cgatcgatga ggggctgatc
     4261 gacgcccttg tctcccgcgg cgcgcacgtc gacctcgtta gcccgcttca gcgtttcccg
     4321 atcttcatcg agagcctcca ccatggcttt gagcgccctt gacttgctgt cgctggaggc
     4381 cgcagcgatc actctggagg cagcgcgagc gctcctggcg acctcgccca tgtaagcggc
     4441 aatgtctgtc atgactttcg ggggcatttg gcccagagat tagggaccgg caagtatacc
     4501 ggaggtcagc gcgcgacgat caaagggcga gaccgagcag caaggtgccc agcatcagcc
     4561 gatagacgac gtagggcagc atccccgtcc gctccaccag cttcacgaaa aagtgtatgc
     4621 agcagtaggc gcctgccccg gcaagcaccg cgccggcgac cagctccgcc catcgaaccg
     4681 catgctcgcc ggcacccaga gacgccgctt ccagcacggc cgcgcccaga atcgtgggta
     4741 tggagagcag aaaggagaac cgggcggcac ccgtacgact caagcccagc agcagcgcgg
     4801 ccgtcatggt aatacccgac cgggaggtgc cgggcacgat ggcgatgacc tgcgcagcgc
     4861 cgatgagcat tgccgccgtc cacgacggat gatcggcgcc gctccggcgc ccgtccgccc
     4921 accacagagc aagcgcaaag acgatggtgg cagaagcgat gacgggcaca gtgcgcaggc
     4981 tggactccac ccagtcgtgc gcgagatagc ccaccagaac gatgggcaac gtcgcggcaa
     5041 tcagcttgag aagcaggtcg gcgtcgtcat cccgctgccc gcgggccagc aaacgggcac
     5101 cactggcagc gaagcggccg atctcgcggc gaaagtaaga cagcacggcc agcagcgtgc
     5161 ccgcatgcac ggctatgtcg aacgccagcc cctgatcggg ccactcggtg agcaatgccg
     5221 ggaggatgag atgagccgag ctggagatcg gcaggaactc cgtgatgccc tgcaccaggg
     5281 caaggatgat gatctgcagc cagtccacgg ctgcgcagtc taacggcaag catccccttt
     5341 cgcgaccgca gcgcgactgc gatgcagcag cgtgctaagg ttctccgatg gagatcccgc
     5401 tgttccccct gcgcgtcgtt cttttcgacg gcggccggtt gcctttgcag atattcgaaa
     5461 cccgctacct ggacatggtg agccgctgca cgcgggagtc gatccccttc ggcgtcgtgc
     5521 tcatacgggg tgcgggcagc gacgctcgcc tggatccgct cgccaaccag cccgcgattt
     5581 tcgaggtagg caccgaagcc cacatcgtcg acttcaatca gctgccgaac ggacggctcg
     5641 gcatcatcgt ccggggtggc cgcaaattcc gcgtggtatc gacccgggag caggccgacc
     5701 acctgctgct cggaacggtg gacttcctgc cggccgagcc cgagagcacc gtgccatcag
     5761 agcacgcccc tctggtcgac atcctcaaag agctggtgaa acatcccggg gtgcagaagc
     5821 tcgggttgga aatcgacttc gccgacgcgc ggtcggtggg cgcccgcctg gccgagctgc
     5881 ttcccataga acccgagatc aagcagagcc tgctgcagct gcagctgccc cgggaacgcc
     5941 tccaggagct gacccggctg gtcaacaaat tccgggacta gaagggaatg tcgtcgtcga
     6001 agtcgtcgtc cgccagcgtt ggcgcgttct cggacgctgc gggcgcagct gattcgaacc
     6061 cgccgccctg gccgccctga ccgcccccgc ggctgtcgag catctgcagg tccctcgcca
     6121 tgacctcagt ccggtagcgt ttctgaccgt cctgctccca gctgctggta cgaaggctgc
     6181 cctcgatgta aaccttcgag cccttgcgca gatactcacc cgcgatctcg gcaaggcgcg
     6241 cgaagcacac cacgttgtgc cactcagtgc gttcctgctg ctcgcccgac tggcggtccc
     6301 gccagttttc cgtggtggcg acgctgaagc tggtgaccgc attgccgccc tgggagtagc
     6361 gcgtctcagg atccctgccc agattgccga tgagaatgac tttgttgata ccccgcgcca
     6421 tagccttttt cccgttgcct cttacccgtt caaatgccca gaaaactcca ggcccgcagt
     6481 ctagcacggc gccgtgaaaa gtccgtaagc gatggtccag cttgcccctg agtagggatc
     6541 ggtttatgtt gccgggtttc cccacttccg gggtcaatca gcgcaagcta gggtcggcga
     6601 gccgcgctgg cagggcataa ggcacgcgca ttggacagca tcgcggttcg cggggcacgc
     6661 acccacaatc ttaaaaacgt caatctggac ataccccgcg atcggctcgt ggtgatcacg
     6721 gggctatcgg gatccggaaa gtcctcgctt gccttcgaca ccctctatgc ggaggggcag
     6781 cgccgctacg tggagtccct ctcggcctac gcccggcagt tcctgtcgat gatggagaag
     6841 ccggacgtcg accatatcga gggcctgtcc ccggccatct ccatcgagca gaagtccacc
     6901 tcgcacaatc cccgctccac cgtgggtacg atcaccgaga tctatgacta tctgcggctg
     6961 ctgtacgcga gggtcggcga gccgcgttgc cccaaccacg acctgcccct tgacgcccag
     7021 acggtcagcc agatggtgga ccaggttctc ggcctgccgg aggacaagcg ggtaatggtc
     7081 ctcgcgcccg tggtcaccga acgtaagggc gagcacctgc atgtctttca ggaactcatg
     7141 gcaaacggct tcatcagagc ccggatcgac ggcatcgtgg tcgatctcga ccacccgcca
     7201 aacctggaga agaacaaaaa gcacaccgtg gaagccgtca tcgaccggct gcgtatccgg
     7261 gcggatgcgc agcagcggct cgctgaatcc ttcgaaaccg ctctagatct cgccgagggg
     7321 atcgcgaagg tggcgtccat ggacgatgcc gacgagccgg aactcgtttt ctcggcaagg
     7381 ttcgcatgtc ccgcctgcgg gtacagcata tccgagctcg agccccgcat gttctccttc
     7441 aacaaccccg ccggcgcctg ccaggcttgc gacggccttg gggtgaagca gtttttcgat
     7501 cccggcctgg tggtccagga cgaggagctg acgctggccg aaggcgccat acgcggctgg
     7561 gaccggcgca acgtttacta cttccacatg ctctcctctc ttgcagccca ctacggcttc
     7621 gacgtggaga cgcctttccg aaagctgccc aaaaagcatc gggacgcaat cctctacggc
     7681 agcggcagag agcggatcga tttcagctac gccaacgacc gcggcgacgt gatccatcgc
     7741 cgccaccggt ttgagggcgt gataccgaac atggagcgcc gttatcacga gaccgactcc
     7801 agcctggtcc gagagaacct gcagaagtac cttcgggtca ggagctgccc cgactgcggc
     7861 ggcacgcggc tgcgcgaggc gtcgcgcaac gtcttcatcg acggcgtcag cctgccggag
     7921 ataacgtccg ggtccgtgga gggctccctg gcctacttcg acgagctcaa gctgtcgggg
     7981 cgacggggag agatcgccga caagatcctc aaggagattc gcgcccgtct gcggtttctg
     8041 gtcgacgttg ggctcaacta tctcaccctg gaccgcagcg ccgagacgct ttcggggggg
     8101 gaagcccagc gcatccgcct ggcaagccag atcggcgccg gcctggtcgg cgtcatgtac
     8161 atcctcgacg agccttccat cggcctgcac cagcgggaca acgcccggct gctcaagact
     8221 ctggagcacc ttcgcgatct gggaaacacg gtgctggtgg tagagcacga cgaggaggcg
     8281 atccgctccg cagaccacat catcgacatc ggaccagggg ccggcgtgca cggcggctgc
     8341 gtggttgccg ccggcagtct cgagaagatc ctgaaatcca ggcgctccat caccggccag
     8401 tatctctccg gtaggaagaa gattcccgtg cctgaaaagc ggacggcgtt cgtcgaggag
     8461 gagaccatcc gcatcatcgg cgccaccggc aacaatcttc agtccgtgtc ggtcgagata
     8521 ccgtgcggcc tgctgacctg cgtaaccggc gtttccggct cgggcaagtc gacgctgatc
     8581 aaccacactc tctaccccgt ggcagctacc agactcaaca aagcaaccac gctgacgccg
     8641 gcacccgtgg aggaggttct gggtctgaat catgtcgaca aggtggtcga catcgaccag
     8701 agccccatcg gccgcacgcc gcgctccaat ccggcaacgt atacgggtct gttcacgccg
     8761 at
//
LOCUS       DBNHKV010000478         5317 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1130836, whole genome shotgun sequence.
ACCESSION   DBNHKV010000478 DBNHKV010000000
VERSION     DBNHKV010000478.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1130836"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="AC16PP_15615"
     CDS             <1..308
                     /locus_tag="AC16PP_15615"
                     /inference="COORDINATES: protein motif:HMM:NF015863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR6483109.1"
                     /translation="IYLDTTEGLLAADGLLMNWTPTNAPAGAIEWGRVTPLSAGEAAA
                     YRQRFRGRMLTAERWLQDLHSGRFFGTPGVIIVDVASLLMLILAGTGIVLWWRSRRG"
     gene            complement(333..1151)
                     /locus_tag="AC16PP_15620"
     CDS             complement(333..1151)
                     /locus_tag="AC16PP_15620"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6483110.1"
                     /translation="MGGYTTREVSDLIGLKPDQVRHYVRRSLLRPARGDRGEYRFNFQ
                     DVVLLRSAKGLLDADVSSRKAYRVLLKLQSELSQVKSLAAVRIFADGNNVVVREDNQV
                     WDVETGQGQLDFTVRELADNVATLAQRNLIVLRESDGLDSDEWYNLGLDLEEVDPDRA
                     PDAYSRAIELNPKNADAHVNLGRLMQLKGNLKFAKRHYELALVAVSNHQLAYYNLGTV
                     YDELDDMDKAAEYYRRAPGVPDAHFNLSRIYQMQGDEVSALRHMRQYRELLDVE"
     gene            complement(1373..2215)
                     /locus_tag="AC16PP_15625"
     CDS             complement(1373..2215)
                     /locus_tag="AC16PP_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010448255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HIR6483111.1"
                     /translation="MSRQASPAGVDEARLEAFLARHRHLLVITGAGCSTPSGIGAYRD
                     EAGEWLRAEPVQMRDFVASEAARRRYWARSMHGWRAFRDAAPNAAHQALADLERRGVV
                     RGLITQNVDGLHQRAGHDDVIELHGSLRRVVCLECGVSREREEVQRWLEARNGFVLDA
                     SASLAPDGDADLVIDDLSAFEVPRCADCGGLLKPDVVFYGDSVPRDRVDACYAGVAQA
                     DAVLVVGSSLMVFSSFRFVRRAVDLGKPVAAVNRGRTRADAWLEFKVDEDCAAVLERI
                     AANL"
     gene            complement(2212..3396)
                     /locus_tag="AC16PP_15630"
     CDS             complement(2212..3396)
                     /locus_tag="AC16PP_15630"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6483112.1"
                     /translation="MLDGAVHPDFADVAAALIRQIPRGRDGGSAVCVYHRGRCVVDVW
                     GGIRDHHGNPWEHDTTAPSFSTTKGVIATLLHILVDHGKAGYDDPIAHHWPEFGARNK
                     ENITIRQALCHEAGLYRVADMIRRPQEMLDWGHMKRVIADAEPAHEPGSSHGYHALTY
                     GWLIGGVIEAVAGRPLQAVLQDELVDPLGLDGIFIGMPHNELHRRAELIHGRVRPPRA
                     TEPWQETLLEWVDAALGQLGVELAEFSAAMDVFSEDFDWNADETAQAIIPAANGQFTA
                     RSLARMYAMIAQGGELDGVRVLSEGRVREITEVRSRTRDRVLFIPMHWRMGYHRAFAF
                     GVSAPHAFGHYGYGGSGAFCDPSRELAVALILNSGAGTPMGGRYMPLVARAAIRAADR
                     CR"
     gene            3547..4992
                     /locus_tag="AC16PP_15635"
     CDS             3547..4992
                     /locus_tag="AC16PP_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008172675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HIR6483113.1"
                     /translation="MSIKWQEYSAEGLWDELITAGGRARPGAGSLCRMLAKLSDEELN
                     ARKAAAELAIRSMGITFTVYTGEGSGSIDREWPFDIIPRLLLKKEWDQISAGLTQRVK
                     ALNCFIDDVYHDQRVFADGVLPREYIEKSREFRGECVGIEPPHRIWSHVCGSDLVRDS
                     DGRVYVLEDNLRVPSGVSYMLENREVMKRVFPELFEDYVIHPVDDYPARLASCLRTLT
                     SVKSPQIAVLTPGIYNSAYFEHAYLANAMAAQLVEGSDLVVGKDDYVYMKTIDGLVRV
                     DVIYRRIDDLFLDPDVFKPDSMLGVPGIVRAWRAGNVALANAPGCGVADDKVIYTYVP
                     DLVRYYLAEEPLLPNVPSYRCVDKEQRDYVLDNLDELVVKPANESGGYGMLIGPKASR
                     AERNRFARLIRANPRNYMAQPMLKLSTAPTLVDEHAEPRHLDLRPFILSGASSYVTMG
                     GLTRVALRKGSTVVNSSQGGGSKDTWVVDLE"
     gene            4989..>5317
                     /locus_tag="AC16PP_15640"
     CDS             4989..>5317
                     /locus_tag="AC16PP_15640"
                     /inference="COORDINATES: protein motif:HMM:NF016086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HIR6483114.1"
                     /translation="MSGHTLSSTAKRTYWLGRYLERTESTARLIAENGNLMIDLPARL
                     PHSWSPLISITGSGATFATLYEEPTERNVTRFLIGDLRNHGSILSSLGWARENVRTLR
                     GIIPRQAV"
BASE COUNT          936 a         1790 c         1712 g          879 t
ORIGIN      
        1 agatctacct cgacaccacc gagggtctgc tggcagcaga cggtctgctg atgaactgga
       61 ccccgacgaa cgcgcccgcc ggcgccatcg agtggggacg cgtgacgccc ctgagcgctg
      121 gggaggcagc ggcctaccgg cagcgattcc ggggcaggat gctgaccgcg gagcgctggc
      181 tgcaggacct gcacagcggg cggttcttcg gcacgccggg cgtgatcatc gttgacgtgg
      241 cgtcgctgct gatgctgatc ctggcaggaa ccgggatcgt gctgtggtgg cgttcgagac
      301 gcggctgaga gccgtgccgg accggccgac ggtcactcga cgtcgagcaa ctccctgtac
      361 tggcgcatgt gcctgagcgc agacacctcg tcaccctgca tctggtagat gcgggagagg
      421 ttgaaatgcg catccggcac gccgggcgca cgccggtagt actcggccgc cttatccatg
      481 tcgtcgagct cgtcgtaaac ggttccgagg ttgtagtagg cgagctgatg attcgagacc
      541 gcgacgaggg caagctcgta gtgtcgcttg gcgaacttga gattgccctt gagctgcatg
      601 agccgcccga ggttcacgtg ggcgtcggcg ttcttgggat tgagctcaat ggcccgggag
      661 tatgcgtctg gagcgcggtc cggatcgacc tcctcgaggt cgaggcccag gttgtaccac
      721 tcgtcgctgt ccagaccgtc gctttcgcgc aggacgatga ggttgcgctg ggccagggtc
      781 gccacgttgt ccgccagctc ccgcacggtg aagtccagct gcccctgccc cgtttccacg
      841 tcccagacct ggttgtcttc ccggaccacc acgttgttgc catcggcgaa gatgcgcacc
      901 gctgccagcg atttgacctg gctgagctcc gactgcagct tcagcagtac ccggtaggcc
      961 ttgcgcgagg aaacgtccgc gtcgagcaga cccttggcgc tgcggagaag cacgacgtcc
     1021 tggaaattga agcggtactc gccgcggtct ccacgagccg gccgcaggag gctgcgccga
     1081 acgtagtgac ggacctgatc gggcttaagc ccgatgagat cagagacttc ccgagtggta
     1141 tacccgccca tcagattggc agatgcggtt ccttcattgg tgcctgaccg aggtgtctag
     1201 ccacggcttc ccgcctcttc gagaccgccg gcggcaggga acgccggcac tatagcgtag
     1261 gggtggtgag ccctcaacac gggccgggca aagtccccgg gcggaggccc gggtggaggc
     1321 gcgtctgcga gccctgctgc gagccgggcc ggaagcccgc cacaaggccc acctagagat
     1381 tggcggcgat tcgttccagc acagcagcac agtcttcgtc gaccttgaac tcgagccatg
     1441 catccgcccg ggttcgcccc cggttcaccg cggccaccgg ctttcccagg tccaccgcgc
     1501 gacgcacgaa acggaagctc gaaaagacca tcagggacga gcccacgacg agaacggcat
     1561 ctgcctgggc aacgcccgcg tagcacgcat ccacccggtc gcggggcacg gagtctccgt
     1621 agaacaccac gtccggcttg agcaagccgc cgcagtctgc acagcgtggc acttcgaagg
     1681 cggagagatc gtcgatgacg aggtccgcat cgccgtcggg cgcaaggctg gcactcgcat
     1741 cgaggacgaa cccgttgcgg gcttccagcc atcgctgcac ctcctcgcgc tcgcggctca
     1801 ccccgcattc gaggcagacg acccgccgca ggctgccgtg cagctcgatg acgtcgtcat
     1861 gtcccgcccg ctggtgcagc ccgtcgacgt tctgggtgat cagtccccgc acgacgccgc
     1921 gcctttccag gtctgccagc gcctggtgag cggcgttggg tgcggcatcg cgaaacgccc
     1981 gccagccgtg catgctccgg gcccagtagc gccgacgcgc tgcctcgctg gcaacgaagt
     2041 cacgcatctg aaccggctcg gcacgcagcc attctcccgc ctcgtcccga taggccccga
     2101 ttcccgacgg cgtgctgcaa cccgcgccgg taatgaccag cagatgccga tgacgggcga
     2161 ggaatgcttc cagccgggct tcgtcgacgc cggccgggct ggcttgccgg ctcaccggca
     2221 ccgatcggcg gcgcgaatcg cggcgcgcgc aaccagcggc atgtagcggc cgcccattgg
     2281 ggtacccgct ccgctgttca ggatgagcgc caccgcaagc tcccggctcg gatcgcagaa
     2341 cgctccgctc ccgccatatc cgtaatgtcc gaacgcgtgg ggcgcgctga ccccgaacgc
     2401 gaatgcccgg tgatagccca tgcgccagtg catggggatg aagagcacgc gatcacgggt
     2461 acggctgcgg acctcggtga tctcgcggac cctcccctcg gacaggaccc tgaccccgtc
     2521 cagctcgccg ccctgggcga tcatggcata catgcgcgca agcgagcggg ccgtgaactg
     2581 accgtttgcc gccgggatga tggcctgcgc cgtctcgtcg gcgttccagt cgaagtcttc
     2641 ggaaaaaacg tccatggccg cgctgaattc cgccagctcc acccccagct gccccaacgc
     2701 cgcgtcgacc cattccagca gggtttcctg ccacggctcg gtggctctag gcggccggac
     2761 gcgcccgtgg atcagctcgg cacgccggtg gagctcgttg tggggcatgc cgatgaatat
     2821 gccgtcgagg cccagggggt cgaccagctc gtcctgaaga accgcctgca ggggacgccc
     2881 ggccaccgcc tcgatgacgc cgccgatgag ccagccgtag gtaagcgcgt gataaccgtg
     2941 ggagctgcca ggctcgtggg cgggctcggc gtcggcaatg acccttttca tgtgccccca
     3001 gtccagcatc tcctgaggcc gccgaatcat gtccgccacg cggtagagcc cggcctcgtg
     3061 gcagagcgcc tggcggatgg tgatgttctc cttgttgcgg gcgccgaact cgggccaatg
     3121 gtgcgcaatg gggtcgtcgt atcccgcctt tccgtgatcc accaggatgt gcagcagcgt
     3181 ggcgatgacc cccttggtcg tggagaacga aggcgcagtc gtatcgtgtt cccagggatt
     3241 accgtgatga tcgcggatgc ccccccagac gtcgacaacg cagcgccctc gatgatagac
     3301 acacaccgcc gagcccccat cgcgccctct gggtatctgc cggatgaggg cggcggcaac
     3361 gtccgcgaag tcgggatgaa cagcgccgtc gagcatggcc gccatcagag tcctgggatt
     3421 agggggcgga cattttatgg aacccgcaag caatgcgcac cagcatcgag aaggctgcca
     3481 tcacaagcgc catgtcccgt agaatgaccc gcgggcaaac ctggcggcgc gcgatcgaat
     3541 tcgagcatga gcatcaagtg gcaagagtac tcggccgaag ggctgtggga cgagctgatc
     3601 acggccggcg gccgcgcccg gccgggtgcc ggcagcctct gccgaatgct tgccaagctg
     3661 agcgacgaag agctcaacgc ccgcaaggcg gcagcggagc tggccatcag atccatgggg
     3721 atcactttca ccgtctatac cggagaggga agcggttcca tcgatcgtga gtggccgttc
     3781 gacatcatcc cccgcctgct cctgaagaag gagtgggacc agatctcggc agggttgact
     3841 cagcgcgtca aggccctgaa ctgcttcatc gacgacgtct accacgacca gcgcgtgttc
     3901 gccgacggcg tgcttcctcg agaatacatc gagaagtccc gggaatttcg aggggaatgc
     3961 gtcggcatcg agcccccgca ccgcatatgg tcacacgtct gcggctccga tctggtccgc
     4021 gactcggacg gccgcgtcta cgtgctcgag gacaacctcc gtgtgccttc cggcgtttcc
     4081 tacatgctgg aaaaccggga agtcatgaag cgcgtctttc cggagctgtt cgaagactac
     4141 gtgatccacc ccgtggacga ctatccagcc aggctcgcga gctgtctgcg cacgcttacc
     4201 tcggtgaaat ccccgcagat cgccgtgctc acgcccggca tctacaactc ggcctacttc
     4261 gagcacgcct acctcgccaa cgccatggcc gcgcagctcg tggagggctc cgacctcgtc
     4321 gtcggcaagg acgactacgt gtacatgaag accatcgacg gcctggtccg cgtcgacgtc
     4381 atctaccgtc gcatcgacga tctctttctg gatccggacg tcttcaaacc ggattccatg
     4441 ctcggcgtcc ccggcatcgt gcgcgcctgg cgcgcgggca acgtggcgct tgccaacgcg
     4501 cccggctgcg gcgtggcaga cgacaaggtg atctacacct acgtccccga cctcgtccgc
     4561 tactacctgg ccgaggagcc tttgctgccc aacgtgcctt cctaccggtg cgtggacaag
     4621 gagcagcgtg actacgtgct cgacaatctg gacgagctgg tggtgaagcc cgccaacgag
     4681 tccggaggct acggcatgct gatcgggccc aaggcgtcac gggcggagcg gaaccggttc
     4741 gccaggctta ttcgcgcgaa cccgcgcaac tacatggccc agcccatgct caagctctcc
     4801 accgcgccca cgctggtcga cgagcacgcc gaaccccggc atctggactt gagacccttc
     4861 attctctccg gcgccagcag ctacgtgacc atgggcggat tgacccgggt cgcgctgcgc
     4921 aagggctcga ccgtggtcaa ctcgtctcag ggcggcggca gtaaagacac ctgggtggtg
     4981 gacctggaat gagcggccat acgctgtcga gcacggcaaa gcgcacctac tggctgggac
     5041 gctacctgga gcgcaccgag agcaccgcaa ggctgattgc ggagaatggc aacctgatga
     5101 tcgatctgcc cgcacggctc ccccacagct ggtcgccgct gatcagcatc accggaagcg
     5161 gagcgacgtt tgccacgctc tacgaagagc ccacggagcg caacgtcacc cgcttcctga
     5221 tcggcgacct gcgcaatcac ggcagcatcc tgtcatcgct gggctgggcc agggaaaacg
     5281 tgaggaccct gcgcgggatc atcccgcggc aggccgt
//
LOCUS       DBNHKV010000479         9488 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_564155, whole genome shotgun sequence.
ACCESSION   DBNHKV010000479 DBNHKV010000000
VERSION     DBNHKV010000479.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 9488)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9488)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9488
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_564155"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="AC16PP_15645"
     CDS             complement(<1..625)
                     /locus_tag="AC16PP_15645"
                     /inference="COORDINATES: protein motif:HMM:NF013954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HIR6483115.1"
                     /translation="MAGNINRFARAPCAALLLMAAACSEAPAPKPDRSYEFESHTDLE
                     ALFAELNYTPEAWADGNRDVPRLYLTSIPSRWRHRTADSLPVTRKKQLFFRLISPAAL
                     RANELVAADRARLLELAKGPGRLAPDDADWLANLAARYRLRDESAAGPEAIEELMLRV
                     DVLPPSLILAQAAEESGWGTSRFADVGNALFGQWAWDDGIIPLERREE"
     gene            complement(638..1192)
                     /locus_tag="AC16PP_15650"
     CDS             complement(638..1192)
                     /locus_tag="AC16PP_15650"
                     /inference="COORDINATES: protein motif:HMM:NF024978.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HIR6483116.1"
                     /translation="MRRCQWGWAVALVLTCAGCAAVAPIGADSDYDSSANFTSYRSFG
                     WISDKPLLLTDATLTSPQFQGRAMKAIRQALEARGYGFEADVENADFAVAFTLGTREQ
                     IRVNAYPDQVRETWTWGGPVPQAADVRNYTEGTLSIDLFDVRLRRPVWHGWATRTISY
                     ADRTNPSPVIAEVVEAILAQFPPQ"
     gene            1305..2204
                     /locus_tag="AC16PP_15655"
     CDS             1305..2204
                     /locus_tag="AC16PP_15655"
                     /inference="COORDINATES: protein motif:HMM:NF024946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HIR6483117.1"
                     /translation="MGERSIFSVCCLILLTTQAARANDVEPRLYSAVPTGANFLSVGY
                     VRSEGEVSLDSSIPVIDAKGEVDSLVLQYSRGLNVAGRSGLLSVVVPYANLALEGIYL
                     GQPASGEVRGFGDPVVRLAVNFTGAPAMTGDEFRGYQQKTIVGASVAVSVPVGRYETE
                     RVLNTSSNRWNVVGQVGFSHRVRRWTLEGAVGLSAFSDNDELKGDNELSQESITLFRG
                     TALYHFTPRVWMSLGVVHSRGGATSLNGVKRRDHQNNWRSGLAFSFPVGRRHRMQLVA
                     TEGVSARIGADFTTFIANYTLSF"
     gene            complement(2259..4475)
                     /locus_tag="AC16PP_15660"
     CDS             complement(2259..4475)
                     /locus_tag="AC16PP_15660"
                     /inference="COORDINATES: protein motif:HMM:NF025875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6483118.1"
                     /translation="MVPIRAALLPLIVSFLSGCDARPPVATDTPDHELEPSTAALTYV
                     GSQQCAGCHAGQHDAWQRSHHRKAMEKPGSHSIVGNFDGASFVHDGTTTEFRAESGSY
                     VVYTDGENGEIARFPVAYTFGVEPLQQYLLPLPRGRLQALSVAWDSRPREAGGQRWFH
                     LYPEDRVDHTDVLHWTQGSQNWNYMCADCHSTNVRKGYDETLDEYDTSFSEVTVGCEA
                     CHGPASAHVDWALTGDGQAGLPSLGGQTAEINACAPCHSRRSQIAEGFRPDLAYLDHY
                     VPALLDEGLYHSDGQILDEVYVYGSFLQSRMHAQGVSCSNCHDPHSAELRATGNDVCT
                     GCHSEAGRADFPTLTKARYDASAHHFHSDGGPGSQCVDCHMPARTYMQVDDRRDHSFR
                     VPRPDLTVELGVPNACTGCHEDRPASWARDVLAERFGKAEREHYGAVFDAARRADPSA
                     ERALAALAEDEARPAIVRATALSLMTAYDRGASSFALSAGLRDESPLVRLGALKGAER
                     WPPDRRWRSAAHLLEDDYLAVRLEAVRVLAPAAGSLPAASRERLNRGVSEYASAQALH
                     GDRAEAHTNMAGVYLNLGEPSLAERALSRAIQINPRWVPALINLADLYRATGRDAAGG
                     GFLDRALSAAPDSGDVLLAKALWLVRQRSQDEALTLLARAAAVEPTSTRIAYVYAAGL
                     HSNDRTQEALEVLDTILATRPGNRQLLEAAAAMARDNGQDGRALGYLEQLKAADGL"
     gene            complement(4573..5286)
                     /locus_tag="AC16PP_15665"
     CDS             complement(4573..5286)
                     /locus_tag="AC16PP_15665"
                     /inference="COORDINATES: protein motif:HMM:NF023197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HIR6483119.1"
                     /translation="MKKNSRIARFALPLAVLLLGACASTTPTINTEAAPDEMSYDGLY
                     PVKGTRVTQAWARSDLDLRSYNKILLQGAGIQYRPVKSTGYRGTAGRSGKNEFPLDEQ
                     QKERVAAAASEAFRSELAKIKSLEIVSEPGPDVLIVRGALLDVVSRVPPEPIGRGNTY
                     LDSVGEATLVIELADSQSGSVLVRAVDRRAAQQTGWMTESTSVTNAAEVRRLFSGWAR
                     LVRTALEELTDDLDIGEAQ"
     gene            5744..8146
                     /locus_tag="AC16PP_15670"
     CDS             5744..8146
                     /locus_tag="AC16PP_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6483120.1"
                     /translation="MVIHAPQIRAWDDFEDIEGLAALELRTDDGAPGRFGTIAFRARA
                     VPDLEARVVHFDDIEVTSVKEQGEEISEEARALFVAAMDLGARDVPLDLVLSHLADGV
                     IPESTTGIRFEPPEILVSTAPAVIVLLHGEPALAPVGDRGALFAANTNWPLFRNGAEG
                     AWYLRDNDNWLTAADLDGPWAWAGELPASLVQLPETDDWRSTRAAAKAWSRPPESEPP
                     SVFVSTEPAELILIDGEPELVEIGTLGLSFVANTESPLFGYSERWYYLVSGRWFTSRA
                     LDGRWSAVLELPKAFAQIPEDHEKADVLAAVPDTPAARMAALDAQIPEKTEVPRDLSM
                     PETVTYVGEPVFEPIPGTNLKRATNTAYSVIQAGADYYLCYSGMWYAASAPEGPWRVT
                     SHVPSQVYQIPPSSPVYNTTYVKVQEESPVSVTFGVTQGYSSVYVSNGVPVYGTGWYY
                     PPYYYYYGGYPIYFAYPYTYGSASFYNPATGTYGSVSRAYGPYGGWGYASAYNPTTGT
                     YGRAEAVWDHDEWYAVGEAYNPHTGRYYTTERYYDADDERWEIDSRFSGERGYVDVSR
                     EFDEDSGRATIETSRGGEGTFSREKSDGGWNTSGEFETAGGRTVTGSGRYEDGEGSST
                     LRGSEGGTGTIERSVDDGVVTRDGTFSKDGKTIETETTRDGTGRRTSFETSEGTSGAV
                     GGRGQQRTALAQTESGDLYAARDGNVYRKSDDGWSRHSDGSWETIPMPSRDADAGARS
                     YGGGDYRQSASSAYRGGADLDADRYRGLDRDYSARQRGMTRAGSYGGQRGSFGRLQGR
                     RR"
     gene            complement(8217..8600)
                     /locus_tag="AC16PP_15675"
     CDS             complement(8217..8600)
                     /locus_tag="AC16PP_15675"
                     /inference="COORDINATES: protein motif:HMM:NF028308.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HIR6483121.1"
                     /translation="MAQRHSRILTLLLLVVVAAPATAFWGWMRGSAANEFKESDWDLF
                     KAEMRQALEDGADGERFDWSNPKTGNSGSIRILETAEVEGETCRKAAFRNVTAKGTQG
                     QSVHYLCRQENGAWQFAAASAFESG"
     gene            8889..>9488
                     /locus_tag="AC16PP_15680"
     CDS             8889..>9488
                     /locus_tag="AC16PP_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR6483122.1"
                     /translation="MRRILLAMGASLLLPAMGLAADKPNILVIWGDDIGQSNISAYTR
                     GMMGYPTPNIDRIAAEGILFTDYYGEQSCTAGRSSFIMGQSVFRTGLSKVGLPGAEEG
                     MQVEDPSIAGLLKDQGYATGQFGKNHLGDRDEHLPTNHGFDEFFGNLYHLNAEEEPEN
                     EDYPKNPEFRKRFGPRGVIQRHSDGRIEDTGPLTKKRMET"
BASE COUNT         1593 a         3066 c         3169 g         1660 t
ORIGIN      
        1 actcctcacg gcgttccaga ggaatgatgc cgtcgtccca tgcccattgg ccgaacaggg
       61 cgttgcccac gtcggcgaag cgcgacgtgc cccagccgct ctcctctgcg gcctgagcca
      121 ggatcagaga tgggggcaga acgtccacgc gcagcatcag ctcctcgatg gcctccggcc
      181 cggcggcact ttcgtccctc agccggtaac gggcggccag attcgccagc cagtcggcat
      241 cgtccggcgc cagccgcccg gggcccttgg ccaactcgag cagccgagct cgatcagcgg
      301 cgaccagctc gtttgcccgc agcgcggcag gactgatcag gcggaagaaa agctgtttct
      361 tccgggtgac ggggaggctg tcggcggttc gatgccgcca tcggctcggg atgctggtga
      421 ggtacagtcg gggcacatcc cgattgccgt ccgcccaggc ttccggcgtg tagttcagct
      481 ctgcgaacag cgcctcgagg tccgtgtggg attcgaactc atagcttctg tccggctttg
      541 gagccggcgc ctcggagcag gcggcggcca tcaacagcag cgctgcgcaa ggtgcccggg
      601 cgaaacgatt gatgttgcct gccattgccg gttccggtca ttgcggcgga aactgcgcca
      661 ggatcgcctc gacgacctcg gcgatcaccg gcgacgggtt cgtccgatcg gcgtaggaga
      721 tcgtcctggt cgcccacccg tgccatacgg ggcggcgcaa acgcacgtcg aacaggtcga
      781 tggacagggt gccctcggta tagttccgca cgtccgcggc ttgaggcacg ggcccgcccc
      841 aggtccaggt ttcccgaacc tggtccgggt aggcgttcac acgaatctgc tcgcgcgttc
      901 ccaacgtaaa agcgaccgcg aaatcggcgt tctcgacatc ggcctcgaag ccatagcccc
      961 tggcttccag agcctgcctg atggccttca ttgcccgtcc ctgaaactgc gggctcgtca
     1021 aggtcgcgtc ggtcagcagc agcggcttgt cggatatcca tccgaagctc cggtaggagg
     1081 tgaaattcgc gctgctgtcg tagtcgctgt cggcgccgat gggcgcgacg gctgcgcacc
     1141 cggcgcacgt caggaccaag gcgacggccc agccccattg gcatcgtctc atttcggtgt
     1201 cctcccgtgg atcccgtcgc ggcgtccgcg cgccgtttcc agaacgccga tggtagcagt
     1261 tttcgctatg atcacgggcc tgcaccgtta ccggcgaggc tggcttggga gagcgctcca
     1321 tcttcagcgt ttgctgcctg atcctgctga cgacccaggc ggcccgcgca aacgacgtcg
     1381 agccgcgcct gtactcggca gtacccacgg gggccaattt cctctcggtg ggctacgtgc
     1441 gatccgaggg tgaagtgtct ctggacagca gcattcccgt gatcgatgcg aaaggagagg
     1501 tggactccct ggtcctccag tactcgcgag ggttgaacgt cgccggcaga tcggggctgc
     1561 tcagcgtggt ggtgccatat gccaacctgg cgctcgaggg gatatacctt gggcagccgg
     1621 cgtccggcga ggtacgcggt tttggagacc ccgtcgtgcg tctggcggtg aacttcaccg
     1681 gcgcgccggc catgaccggg gacgagttcc gcggctacca gcagaagacc atcgtcggtg
     1741 ccagcgttgc cgtctcggtg ccggtggggc gctacgaaac cgagcgtgtc ctcaacacga
     1801 gcagcaatcg ctggaacgtc gtcggccagg tgggcttctc ccatcgggtt cgtcggtgga
     1861 ccctggaagg cgccgtcggc ctatcggcgt tctccgacaa cgacgagctc aagggcgaca
     1921 acgagctgtc tcaggagtcc ataacgctgt tccgcggcac cgcgctgtat cacttcaccc
     1981 cgcgggtctg gatgagcctt ggcgtggtac acagccgcgg aggtgccacc agcctcaacg
     2041 gtgtaaagcg tcgcgatcat cagaacaact ggcgctcggg cctggctttc tcgttccccg
     2101 ttggccgcag acacaggatg cagctggtgg ccacggaagg ggtcagcgcg cgaatcggcg
     2161 ccgacttcac cactttcatc gcgaactaca ccctctcttt ctgagtcctc tggggagccg
     2221 ttggcgcgcg taagatccgc caggcgggca gggttcgctc agagaccgtc ggctgctttt
     2281 agctgctcca ggtatccgag cgctcggcca tcctggccgt tatcccgggc catggccgct
     2341 gctgcttcga gcagctgtcg gttgccaggg cgggtggcga ggatggtgtc gagcacctcc
     2401 agggcctcct gcgttcggtc gttcgagtga agcccggcgg cgtacacgta ggcgattcgg
     2461 gtgctggtcg gctcgacggc tgctgcgcgg gcaagcaggg tgagggcctc gtcctgagac
     2521 cgttgccgca ccagccacag cgccttggcg agcagcacgt ccccagagtc gggtgctgcg
     2581 ctgagcgcac ggtccagaaa cccgcccccc gcggcatcac gccccgtcgc tctgtacagg
     2641 tcggccaggt tgatcagagc cggcacccac cggggattga tctggatggc ccgggatagc
     2701 gcccgttcgg ccaggctcgg ttcgcccagg ttcagataga cgccagccat gttcgtgtgc
     2761 gcctccgccc gatccccatg cagcgcctga gcggaagcgt attccgacac accgcgattc
     2821 aggcgttctc gtgatgccgc cgggagcgag cctgctgccg gggccagcac gcggacggcc
     2881 tccagccgca cggcgaggta gtcgtcctcc agcaggtgcg ccgccgaacg ccagcggcga
     2941 tcgggcggcc agcgctcggc ccctttcagg gcaccgaggc gcaccagggg gctttcgtcc
     3001 ctcaagccgg cgctcagcgc aaacgagctg gcgccacggt cgtaagcggt catcagggaa
     3061 agcgcggtgg cgcgaacgat ggccggccgg gcctcatcct cggccagcgc ggcgagcgct
     3121 cgctctgcgc tcggatccgc gcgacgggcc gcgtcgaaaa cggcgccgta gtgctcgcgt
     3181 tcagccttgc caaagcgctc tgccaacacg tccctcgccc aactggccgg gcgatcctcg
     3241 tggcagccgg tgcaggcgtt cggcacaccc agctcgacgg tcagatcagg ccggggaacc
     3301 cggaagctgt gatccctccg atcgtcgacc tgcatgtagg tgcgggcagg catgtggcag
     3361 tccacgcatt gcgagccagg gcctccatcc gaatgaaaat ggtgggcgga tgcatcgtag
     3421 cgagcctttg tgagcgtggg gaagtcggcc cggcccgctt cggagtggca cccagtgcac
     3481 acgtcgttgc cagtggcgcg cagctcggcc gagtgcgggt cgtggcagtt gctgcagcta
     3541 accccttgtg catgcatcct gctctgcagg aacgatccgt agacgtacac ctcgtcgaga
     3601 atctgcccgt cgctgtggta gagcccctcg tccagcaggg cgggaacgta gtgatcgagg
     3661 taggcgagat ccggccggaa cccctcggcg atctggctgc gtcggctgtg acagggcgcg
     3721 caggcgttga tctcggcggt ctgaccccca aggctgggaa ggccggcttg gccgtcaccg
     3781 gttagcgccc agtcgacgtg cgcggacgcc gggccatggc aggcctcgca gcctacggtt
     3841 acctcgctga agctcgtgtc gtactcatcc agcgtctcgt cgtaaccctt gcgtacgttg
     3901 gtggagtgac agtcggcgca catgtagttc cagttctggc tcccctgggt ccagtgcaga
     3961 acgtccgtgt gatccacccg atcctcggga tagaggtgga accagcgttg cccgccggct
     4021 tcccggggcc gcgagtccca ggccacgctc aacgcctgca ggcgaccgcg cgggagaggc
     4081 agcagatact gttgcagcgg ctcgacgccg aacgtgtagg cgacggggaa gcgggcgatc
     4141 tcgccgttct cgccatcggt gtacaccacg taggaacccg attccgcgcg aaactcggtg
     4201 gtggtcccgt catgaacgaa cgaggcgccg tcgaaattgc cgacgatgct gtgggagcct
     4261 ggcttttcca tggctttgcg atggtgcgag cgctgccagg cgtcatgctg gccggcgtga
     4321 cagcccgcgc attgctggga gccaacgtat gtgagcgccg ccgtcgaggg ctcgagttcg
     4381 tggtccgggg tgtcagtggc gaccggtggg cgggcatcgc agcccgacag gaagctcaca
     4441 atcaggggca ggagagcggc tcggatcggg accatggcag gcgacggggt cagcgagcgt
     4501 taaccgtcac ccatcgcccc gggaggacgc gatgcgcctg aacccggggc gagagggcgg
     4561 aagatggctc agctattggg cttcgccgat gtccagatcg tcggtcagct cctccagggc
     4621 cgtgcgcacc aggcgagccc aaccgctgaa cagacgccgc acttccgcgg cgttggttac
     4681 cgaggtggac tccgtcatcc agccggtctg ctgggcggcg cgccgatcca cggcgcgcac
     4741 cagcacgctg ccgctttggg aatccgccag ctctatgacc agcgtcgcct cgccgacaga
     4801 atccaggtag gtgttgcctc gaccaatggg ttccggcggt acccgggaca ccacgtcgag
     4861 cagcgcgccg cgcacgatga gcacgtcggg cccgggctcc gaaacgatct ccaggctttt
     4921 gatcttcgca agctcgctgc ggaaagcttc gcttgcggcc gcggcgacgc gctctttctg
     4981 ctgctcgtcc aggggaaact cgttcttgcc gctgcgaccg gcggtgcccc ggtatccggt
     5041 cgacttgacc ggtcgatact gaatgcccgc tccctgcaga agaatcttgt tgtagcttct
     5101 caggtcgagg tccgagcggg cccaggcctg ggtaacccgg gttcccttca cgggatacag
     5161 gccgtcgtag gacatctcgt ccggggccgc ctcggtgttg atggttggtg tcgtgctcgc
     5221 gcaggccccc agcagcagga cggccagcgg cagcgcgaac ctggcgatgc ggctgttctt
     5281 tttcatcgcg gattccccct tttttgtcat tggtggacgg ttgctttcca tgtcgtgagg
     5341 ggccgatgtt agcgtcaagc taaccgaaag caaggcggcc tactggcagg tggcctcgca
     5401 gcccgcagcc tgggcgtccc ctgggcagtc accgcgttcg ggttgtcgtc gtgggcacga
     5461 tcacggcttt cgactacact gcccggtctc ctgcgcagac cccccgagga acgttatgga
     5521 ccgctgggca ccaacaggcc ttgcgttgat cagttcgctg ttgcttgtga gcgcgtcgcc
     5581 cgtcccgtcg tggctgatgc tgagttcggc tcaggcgtca gagccggcgg ctggcccgtc
     5641 tgccaccgcg tctgccaccg cgtctgccac cgcgtctgcc ggcaagactg aagcgccgac
     5701 gccagggcag tttccgaggt ccgtggatgt cgagggcggg ttggtggtga ttcacgctcc
     5761 gcagattcgc gcgtgggatg atttcgagga catcgaggga ctcgcggcgt tggagctgcg
     5821 cacggacgac ggcgcccccg gccggtttgg caccatcgcg ttcagggcaa gggccgtccc
     5881 ggacctggaa gcccgcgtgg tccactttga cgacatcgag gtcacctcgg tgaaggagca
     5941 gggcgaagaa atctccgagg aggcacgggc gctgttcgtg gcggccatgg atttgggggc
     6001 ccgggacgtt ccgctcgacc tggtcctgag ccacctggct gatggggtga tcccggagtc
     6061 caccacgggc atccgcttcg agcctccaga gatactcgtt tccaccgccc cagccgtgat
     6121 cgtccttttg catggcgagc cggcgctcgc gcctgtgggg gatcggggag cgctgtttgc
     6181 tgccaatacc aactggcccc tgttcagaaa cggcgccgag ggagcctggt acctgcggga
     6241 caacgacaac tggctcaccg ccgccgatct cgacggtccg tgggcttggg cgggtgagct
     6301 tccggcctct ctggttcagc tgccggaaac ggacgactgg cgctccaccc gcgcagcggc
     6361 taaggcatgg tcgcggccgc cggaaagcga gcccccatcg gttttcgtat ccaccgagcc
     6421 ggccgagctg atcctgattg acggcgagcc ggaactggta gaaatcggca cccttggcct
     6481 gtccttcgtc gcgaataccg aaagcccgct gttcggctac tccgagcgct ggtactacct
     6541 cgtttccgga cgctggttca cgagcagggc gctcgatggc aggtggtcgg ccgtgctcga
     6601 gttgcccaag gcgtttgccc agattcctga agatcacgaa aaggctgacg tgctggccgc
     6661 ggttcccgat acgccagcag ccagaatggc cgctctggat gcccagattc ccgagaagac
     6721 cgaggtgcct cgggatctca gcatgccgga aaccgtgacc tatgtgggcg aaccggtgtt
     6781 cgagcccatc ccgggcacca acctcaagcg tgcgacgaac accgcctact cggtcattca
     6841 ggccggcgcg gactactacc tgtgctacag cggcatgtgg tacgcggctt cggcgcccga
     6901 agggccctgg cgcgtgacca gtcacgtgcc ctcccaggtc taccagatcc cgcccagctc
     6961 gccggtctac aacaccacct acgtgaaggt tcaagaggaa agcccggtct cggtgacctt
     7021 cggcgtgacc cagggctact cgagcgtgta cgtctccaac ggggtgcccg tctacggaac
     7081 cggctggtac tacccgccct actactatta ctacggcggc tatccgatct acttcgctta
     7141 tccgtacacg tatggctccg cctctttcta caacccggcc acgggcacct acggcagcgt
     7201 ttcgagggcc tacggacctt acggcggctg gggttacgcc tcggcctaca acccgaccac
     7261 gggcacttac ggtcgggcgg aggcggtttg ggatcacgac gagtggtatg cggtgggcga
     7321 ggcctacaac ccccacaccg ggcggtacta cacaaccgag cggtactacg atgccgatga
     7381 cgagcgctgg gaaatcgact cccggttctc cggcgagcgt ggttacgtag acgtgtccag
     7441 agagttcgac gaagactccg gtcgcgccac cattgaaacg tcccggggcg gggaaggcac
     7501 cttctctcgc gagaaaagcg acgggggctg gaacaccagc ggcgagttcg agaccgccgg
     7561 cggccgcacc gtcaccggca gcggccgcta cgaagacggc gaaggaagct ccacgctgcg
     7621 tggctccgaa gggggtaccg gcaccataga aaggtcggtg gatgatggcg tggtgacccg
     7681 ggacggcacc ttttccaagg atggcaagac catcgagacg gaaaccaccc gcgacgggac
     7741 ggggaggcgc acgtcgttcg agacctcaga aggcaccagc ggcgcggtcg gcggccgggg
     7801 acagcagcgc acggcgctgg cgcagaccga gtccggtgat ctgtacgcgg cgcgagacgg
     7861 gaacgtctac aggaaatcgg atgacggctg gtcgcgtcac agcgacggca gctgggagac
     7921 catcccgatg cccagccgcg acgccgatgc cggcgcccga tcctatggcg ggggcgatta
     7981 ccgccagtcc gcgtctagcg cctatcgagg cggcgcggat cttgatgccg atcgctatcg
     8041 cggcctggat cgcgattact ccgcccgaca gcgcggcatg acccgcgccg gctcgtatgg
     8101 gggacaacgc ggcagcttcg gtcggctgca gggccggcgc cgttagcacc agccggtacg
     8161 gcctggaaat acggttgcta ggtgccggat cgggagcggt ccaggggcga ttgggatcag
     8221 ccggattcga acgcgctggc cgcggcaaac tgccaggcgc cattttcctg tcggcaaaga
     8281 tagtggacgg attgcccctg ggtacccttg gcggtcacgt tgcggaacgc cgccttgcga
     8341 caggtctcgc cttccacctc ggcggtctcg agaattctga tcgacccgct gttgccggtt
     8401 ttcgggttgc tccagtcgaa gcgctcgccg tcagcgccgt cttccagggc ctggcgcatt
     8461 tccgctttga acagatccca gtcgctctcc ttgaactcgt tggcggccga cccgcgcatc
     8521 cagccccaga aggccgtggc aggcgccgca acgacgacca gcaggagcag ggtaaggatt
     8581 cgcgagtgcc gctgcgccat gatgttttcg accccgtcga aaaaggagtc aagtctagcc
     8641 taaggtcgcg gatctgctcc accgatcccg gcctggcagc cggcgcagcg cttccggcgg
     8701 cgagggggtg aggtgactcg atgcgccagg tttagccggt ttgcctgtcc acgagacccg
     8761 ggtatcatgc gggcggcgcg cgacggaccg agacgaaaat gctatgtgcg gcgcgctgca
     8821 gatgcctcga agctcgctat gggcgggctc aacgaaaatc catttttgaa acgctaggga
     8881 gtttacacat gcgacgcatt ttgctcgcga tgggcgcgtc tttgctcttg cccgccatgg
     8941 ggctggctgc cgacaagcca aacatactcg tcatctgggg cgacgacatc ggtcaaagca
     9001 acatcagtgc ctacacccgc ggcatgatgg gctaccccac gcccaacatc gaccggatag
     9061 cggccgaggg tatcctgttc accgactact acggcgagca gagctgcacc gccgggcgct
     9121 cgtccttcat catgggtcag agcgtcttcc gtaccgggct gtcgaaggtc ggtctgccgg
     9181 gcgcggagga aggcatgcag gtggaggacc cctccatagc cggcctgctg aaggaccagg
     9241 gctacgctac gggccagttc ggcaagaacc atctgggtga ccgagacgag cacctgccca
     9301 ccaatcacgg tttcgacgag ttcttcggca atctgtatca cctgaacgcc gaagaggaac
     9361 ccgagaacga ggactacccg aagaatcccg agtttcgcaa gcgattcggg ccacgtgggg
     9421 tgatccaaag acattccgat ggccggatcg aagatacggg accgctgaca aaaaagcgga
     9481 tggaaacc
//
LOCUS       DBNHKV010000480         2082 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_141084, whole genome shotgun sequence.
ACCESSION   DBNHKV010000480 DBNHKV010000000
VERSION     DBNHKV010000480.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_141084"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="AC16PP_15685"
     CDS             <1..599
                     /locus_tag="AC16PP_15685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6483123.1"
                     /translation="PEGCSGSPIPKIGYFGWKTWELAFDGCRIPKENIITGGGGDGFK
                     NAMHFLNAARAHTAARSIGLARGALEDAMAYSHERVQFGVPIADFQETRFKIARMASE
                     IEAARQLMYHAATLLDAGETAERESAMVKWYASEMAERVTSDALQILGGAGYTKLHAV
                     ERHWRDARLTKIFEGTSEIQLRIISDRLLGKPSHRATA"
     gene            696..>2082
                     /locus_tag="AC16PP_15690"
     CDS             696..>2082
                     /locus_tag="AC16PP_15690"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR6483124.1"
                     /translation="MGDKDPGDLPTTFGELKSSGYQVLPVKEELRRNLISRLRSRETL
                     FPGVMGYDDTVVPQLTNAILGSQDIILLGERGQAKSRMVRSLTSLLDEWTPVLAGTEI
                     PENPFQPITAQGRQILEAQGDSAPIRWLHRDERYAEKLATPDITIADLIGEVDPIKVA
                     EGRYLSDELTLHYGLVPRVNRGIFAINELPDLSERIQVGLLNLLEERDVQIRGHKVRL
                     PLDVFIVATANPEDYTNRGRIITPLKDRYGAQIRTHYPADVDQEVAIMEQEAAVADMG
                     DYRLEIPAFMKEIVAEITHHARRSPDINQRSGVSVRTSIADYEAMLANAFRRALRLGE
                     DEVVPRISDLAFVLPSFQGKVEFEAMEEGQEDKITQRIMQAAIKSVFDRYYDVNDLES
                     IALAFNDGLTVDTGEATPAADYNAIGERIPGLMDVFPDREGVSPGVRAAIIEFILEGL
                     HLHKLLNKFDNR"
BASE COUNT          405 a          696 c          661 g          320 t
ORIGIN      
        1 tgcccgaggg ctgctccgga tcacccatac ccaagatcgg ctacttcggg tggaagacgt
       61 gggagctggc cttcgacggc tgccggatcc ccaaggagaa catcatcacc ggcggtggcg
      121 gtgacggttt caagaacgcc atgcactttc tgaacgccgc cagagcccat accgccgccc
      181 gctccattgg tctcgcccgg ggggcgctgg aggacgccat ggcctactct cacgagcggg
      241 tgcagttcgg cgtgcccatc gccgacttcc aggagacccg cttcaagatc gcccgcatgg
      301 ccagcgagat cgaggcggcc cggcagctga tgtaccacgc tgccacgctg ctagatgccg
      361 gcgagaccgc ggagcgggaa tcggccatgg tgaagtggta cgccagcgag atggcagagc
      421 gggtcacctc cgacgccctg cagatcctgg gcggagccgg ctacacaaag ctgcacgcgg
      481 tggagcgtca ctggcgcgac gcgcgtctca cgaagatctt cgagggcacc agcgagatcc
      541 agctcaggat catttccgat cggctgctgg gcaagcccag ccaccgcgca accgcctgag
      601 cgccccagcg ggcggcgcgg cgccggcgtg attgccaagc gccagacggg ctgcgagact
      661 agatgcgaca gacttgacgc ggggcgctgc aacgaatggg ggacaaagat cccggagacc
      721 ttccaacgac gttcggcgag ctgaagtcct cgggctatca ggtgctgccg gtaaaggaag
      781 agctccgccg taacctgatc agccgcctgc gcagccgcga gaccctgttt ccaggggtca
      841 tggggtacga cgacacggtc gtgccccagc tcaccaacgc cattctcggc agccaggaca
      901 tcatcctgct cggcgagcgc ggccaggcca aatcgcgcat ggtgcgcagc ctcaccagtc
      961 ttctggacga gtggacgccc gtgctcgcgg gaacggaaat tccggagaac ccctttcagc
     1021 ccatcaccgc ccagggtcgg cagatcctgg aagcccaggg ggacagcgcc cccattcgct
     1081 ggctgcaccg ggacgagcga tacgccgaga agctcgccac tccggacatc accatcgcgg
     1141 atctcatcgg cgaggtcgac cccatcaagg tggccgaagg ccgctatctc tccgatgagc
     1201 tcaccctgca ctacgggctc gttccgcgcg tcaaccgggg catattcgcc atcaacgagc
     1261 tgcccgacct gtccgagcgc attcaggtgg gacttctgaa tctcctcgaa gagcgcgacg
     1321 tgcagatcag gggtcacaag gtccggctgc cgctggacgt cttcatcgtg gccaccgcga
     1381 accccgagga ctacaccaac cgcggccgca tcatcacccc gctgaaggac cgctacggcg
     1441 ctcagatccg gacccactac ccggcggacg tcgatcagga agtggccatc atggagcagg
     1501 aagcagccgt ggccgacatg ggcgattacc ggctcgaaat ccccgccttc atgaaggaga
     1561 tcgtcgcgga gatcacccat cacgcccggc gctcgcccga catcaaccag cgatcgggcg
     1621 tttccgtgcg aacgtccatc gccgattacg aagcgatgct ggcgaacgcc ttccggcggg
     1681 cgctgcgcct cggcgaggac gaggtggtgc cgcgcatctc cgatctggcg ttcgtgctcc
     1741 cgtcgtttca gggcaaggtc gagttcgagg ccatggagga aggccaggag gacaagatca
     1801 cccagcggat catgcaggcc gccatcaagt cggttttcga tcgctactac gacgtgaacg
     1861 atctggagtc cattgccctg gctttcaacg acgggctcac ggtggatacc ggcgaggcca
     1921 cacccgcagc ggactacaac gccatcggcg agcgcatacc cggcctgatg gacgtgtttc
     1981 ccgaccggga gggcgtgagc cccggcgtgc gggcggccat catcgagttc attctggaag
     2041 ggctgcacct gcacaagctt ctcaacaagt tcgataaccg gg
//
LOCUS       DBNHKV010000481         1861 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_66141, whole genome shotgun sequence.
ACCESSION   DBNHKV010000481 DBNHKV010000000
VERSION     DBNHKV010000481.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_66141"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1214
                     /gene="rodA"
                     /locus_tag="AC16PP_15695"
     CDS             129..1214
                     /gene="rodA"
                     /locus_tag="AC16PP_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR6483125.1"
                     /translation="MGAGRRLVQIHLDPLLLLLLLAIVGYGLVVLYSALNGNEELFSN
                     QAVRIGLALSVMLVAAQLEPRFYLRWSPVVYGVGLVLLVLVLLIGVKVKGSQRWLDLP
                     GLPRFQPSELMKIAVPMMVAWYFHERRLPPSFRDVCVAIVIIGLPAGCIILQPDLGTG
                     ILVACAGFAVVVLAGVYWRWIAFAAFGIAALAPGLWFLLQDYQRQRILTLFDPESDPL
                     GAGWNIIQSTTAIGSGGVWGKGLMQGTQSRLDFLPESQTDFIIAVIGEELGLVGIASL
                     LVLYLVVVARGLYIATRAVNTYQRLLAGALTLTFFVYVFVNIAMVSGLLPVVGVPLPL
                     VSYGGTSALTLMASFGIIMAIRTERAW"
     gene            1271..>1861
                     /locus_tag="AC16PP_15700"
     CDS             1271..>1861
                     /locus_tag="AC16PP_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010174785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HIR6483126.1"
                     /translation="MRYAFLWATTLLLLFGSAHADFAGRAEVREYIAEMVREHGFGEE
                     ELADVFADADRKESILEAIARPAERVLKWHEYRRIFLGEKRISQGIEFWRQHAETLAR
                     AEAAYGVPPEYVVAIIGVETRYGRNRGGYRVVDALTTLAFDYPPRSAFFRDELTHFLL
                     LAREEGMDPRDPKGSYAGAMGYGQFIPSSYRCYAVDF"
BASE COUNT          267 a          575 c          606 g          413 t
ORIGIN      
        1 aatggcgggg gtggcagctc ggtggcggga cccgttgccc gtgaggtcat cgatgctttc
       61 atgcttcctc agctggccgc gcggtgagca atcaggactt cgtcaggcac ctccccggcg
      121 gccgggccat gggcgcaggt cgccgtctgg tgcagattca cctcgacccg cttctgcttc
      181 tgcttctgct ggccatcgtg ggctacggac tggtggtcct ctacagcgcc ctgaacggca
      241 acgaggagct gttttcgaat caggctgtgc gcatcggcct ggcgctctcg gtgatgctgg
      301 tggcagcgca gctggagccg cgcttctatc tgcgctggtc gcccgtcgtc tacggcgtgg
      361 gcctggtgct tctcgtgctc gtcctcctaa tcggtgtgaa ggtcaaaggc tcccagcggt
      421 ggctcgacct gcctggactg cccagattcc agccttcgga gctcatgaag atcgccgtgc
      481 ccatgatggt ggcctggtac ttccatgagc gacgcctgcc gccgagcttc cgcgacgtct
      541 gtgtcgccat cgtcatcatc ggcctgccgg ccggttgcat catcctgcag ccggatctgg
      601 gaacgggcat cctcgtggcc tgcgcgggct ttgccgtggt ggtgctggcc ggcgtctact
      661 ggcgatggat agccttcgcc gcgttcggca tcgccgccct tgccccggga ctttggttcc
      721 tgcttcagga ctatcagcga cagcggatcc tcaccctgtt tgatcccgaa agcgatcccc
      781 tgggcgccgg ctggaacatc attcagtcga cgacggccat cgggtctggc ggcgtgtggg
      841 gcaaggggct gatgcaaggc acgcagagcc gcctggattt cctgcccgag agccagacgg
      901 atttcatcat cgccgtcatc ggcgaggagc tcgggctggt tggcattgcc tctctgctcg
      961 ttctttatct cgtcgtggtc gcccgggggc tgtacatcgc gacgcgcgcg gtgaatacgt
     1021 atcagcgcct gctggcaggc gcgctgacgc tgacgttctt cgtttacgtg ttcgtcaaca
     1081 tcgctatggt gagcgggctg ctgccggtcg tcggcgtgcc gctgccgctg gtcagctacg
     1141 gaggcacgtc tgcgctcacg ctcatggcga gttttggcat catcatggca atccgcaccg
     1201 agagggcatg gtagtgcgcc tggtttcggg tcccttcttt cacattggac acgttcggat
     1261 aggtagattc atgcgatacg cttttctctg ggcaaccacc ctccttctcc tgttcggctc
     1321 cgcgcacgcg gactttgccg gtcgggctga ggtacgcgag tacattgccg agatggtccg
     1381 ggagcacggt ttcggggagg aggaacttgc cgacgtcttt gccgatgccg atcgcaaaga
     1441 aagcatcctc gaggccattg cccgtcccgc cgagcgggtg ctgaagtggc acgagtatcg
     1501 gcggatattc ctcggagaga agcgcatctc tcagggtatc gagttctggc gccagcacgc
     1561 cgaaaccctg gcacgagcag aagcggccta tggcgtgccg cccgagtacg tcgtggcgat
     1621 catcggggtg gagactcgct acgggcgcaa tcggggtgga taccgggtcg tggacgctct
     1681 gacgacgctc gcctttgact acccgccgcg ctccgctttc tttcgtgacg agctcaccca
     1741 ttttctgctg ctcgcccggg aggagggcat ggatccgcgg gatccgaaag gctcctatgc
     1801 cggcgccatg ggatacggtc agttcattcc ttccagctat cgctgctatg ccgtggactt
     1861 t
//
LOCUS       DBNHKV010000482        13486 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_67263, whole genome shotgun sequence.
ACCESSION   DBNHKV010000482 DBNHKV010000000
VERSION     DBNHKV010000482.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 13486)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13486)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13486
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_67263"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="AC16PP_15705"
     CDS             <1..615
                     /locus_tag="AC16PP_15705"
                     /inference="COORDINATES: protein motif:HMM:NF017634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HIR6483127.1"
                     /translation="LPLGPSEVLVEDTRYSVTPGRDALSDESAVRGYAQARGWQVQSV
                     RGREEGVLPITLGGDIEGLWQDAAGMPLIGLRAALFHATTGYSFADAVRTADAIATSA
                     DLSSEALGGLIRRRSIRHWRGQSYLRFLNRMLFLAARPGQGYRVMERFYGLPQGLIER
                     FYADRLTSLDRLRILTGKPPVAILPALRCLSESSVKRFHSGIEF"
     gene            612..2156
                     /locus_tag="AC16PP_15710"
     CDS             612..2156
                     /locus_tag="AC16PP_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007253234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HIR6483128.1"
                     /translation="MNTSQRPTAAVIGSGFGGLALAIRLQSAGFRTTLFEGRDRPGGR
                     AYVYEDEGFTFDAGPTVITDPDCLRELFALSGRSLEDYLELTPIAPFYRLCWEDGYSF
                     DYSNDPDETAAQIGAKNPADVDGYRRFLAYSEEVLKEGYVKLGSVPFLDFASMIKAAP
                     QLVRLQSYRSVYSMVSRFVKDEQLRQAFSFHTLLVGGNPFNTSSIYTLIHSLEQRWGV
                     HFPKGGTGALVQGLVRLFGDLGGELKLNTPVAEILTSGNRVTGVRTDNGWEGRFDAVA
                     SNADVVFTYERLLGRTARGRAAARALRRKRFSPSLFVVYFGVRGSYPELKHHSILFGP
                     RYRELLKEIYAGPGLPDDFSLYLHAPSVTDPSLAPPGCSTFYVLSPVPNLKAADLDWS
                     ELGPRYRDHILAYLEKHYLPGLGERLVTTRTFTPDDFQTQLNAHLGSAFSLEPVLWQS
                     AWFRTHNRDDVLKGLYFAGAGTHPGAGIPGVVGSAKATSRLMLDDFGRTYPDSDRSDQ
                     FAKEMA"
     gene            2157..3086
                     /locus_tag="AC16PP_15715"
     CDS             2157..3086
                     /locus_tag="AC16PP_15715"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003293992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HIR6483129.1"
                     /translation="MSDVFAASASIISRGSKSFAAAARLFDPTTRGHAIMLYAWCRHC
                     DDEIDGQDLGIGQRSPAPPEQRARLEQLRRDTEAAMRGEAVDGTPFVAFQRVFLECDI
                     PRNYPLELLDGFAMDVEGHECRTLSDTLRYCYHVAGTVGGMMATVMGIRDPEVVARAV
                     DLGIAFQLTNIARDVMQDAADGRCYLPRQWLADAGVPEDTGQFPDHRDVIYGQTIRIL
                     DEAERYYESARHGIGRLRFRNAWAIASALAIYRDIGRVVRRDGPDVWQHRATVGVGRK
                     LVLLLKGAALAGLQVTFGRRRTAPIRRGLWSAS"
     gene            complement(3075..3560)
                     /locus_tag="AC16PP_15720"
     CDS             complement(3075..3560)
                     /locus_tag="AC16PP_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020305761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR6483130.1"
                     /translation="MTVVVNAACFLTALAIMEWVAWVTHKYVMHGFLWSLHESHHQPR
                     KGRFERNDWFAVFFAVPSIVLIYLGNNGYPAALWGGLGVAAYGLIYFIFHDVIVHRRL
                     DTGYAPRSRYMRRIVHAHRLHHAVSEKHGAVSFGFVYAPPIPVLRAEMKALAASGASG
                     S"
     gene            complement(3557..4459)
                     /locus_tag="AC16PP_15725"
     CDS             complement(3557..4459)
                     /locus_tag="AC16PP_15725"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR6483131.1"
                     /translation="MDAIRHSVEARLRELSPSAAEGGERIEHAIQYSLLDGGKRLRPL
                     LTILTASTFDADPDLALDPACAIEMVHTASLVLDDLPCMDDALQRRGKPATHVAHGED
                     IAILSAVSLISEAFGVIARAPGIAPEVRTSIVGRLADAIGIQGLSAGQERDLHEAQST
                     TSRAALEKRHHQKTGALFVACLEAGARIAGVDETTMAAMTKFGERVGIGFQILDDLLD
                     LSASESTGKDAHRDEGKATFATVMTIQEAEREAITCLDNALAELADQIADAGPFAVFV
                     DLVVRAYADQVSRRGSEIAGALRP"
     gene            complement(4567..6102)
                     /locus_tag="AC16PP_15730"
     CDS             complement(4567..6102)
                     /locus_tag="AC16PP_15730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR6483132.1"
                     /translation="MINPATPRAAGVWPFCVAAKVLVLVIAGTQYGFLADELYLLDAA
                     ARPAFGYVDFPPLTPWLLALLGWIGGTDLIWLRGLACAAGIVTTLVAVDVCRILGGRN
                     FAQWTTAIVVLFAPAFMSVQSIFTMNVLDQLWWLLALSVLLRYLHDHQPRYMILLGVI
                     FGLALLTKLAIAAFILCLLGTSALYARWMFYRTESWAGSAIALSMLAPFVMWQAVSGW
                     PLLEFVQAYNVSEPEPMVLDQPLFGLIATMNPPFLLIWLPGLIYSLMAPKPIRVVGSA
                     AVATLVFFLAAGVKFYFATPLFALFTALGAVLWQRWLSSSGARSCLLAILLISGVAAV
                     PIAAPVLAPTMLQQVANFIRDAEQDKVADDDAPIGRYFPHFAEMHGWPELTEWVTRAY
                     EGLPSARRPEVAVVAAYFGQAGALNQLDKADRLPVVHSGHMNYYLWWQADEPNARLED
                     VLLVGFHPKELEPLFAEITILDRFSCNRCMTREQGAYLIRARRPLIDPEEIRDRLKRF
                     HFF"
     gene            complement(6099..7160)
                     /locus_tag="AC16PP_15735"
     CDS             complement(6099..7160)
                     /locus_tag="AC16PP_15735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6483133.1"
                     /translation="MPTFNEADNVGMVVERLDACLGDHRWEVIFVDDDSPDETAGEVR
                     RLAQSDGRVRCVQRLDRRGLSSACIEGMLASSAPYIAVMDGDLQHDESLLPGMLNALK
                     ADDLDIVVGSRYVSGGDAEAFTRDRARKSRVATRLSRMVVPDELRDPMSGFFMIRREA
                     FEASMRQLSGIGFKIMMDLFASSPGPMRFREFPYSFRTRSQGESKLDNQALWDYGMLL
                     LDKLFGRYVPVRFVSFMLVGGFGVLVHMAVLFVLYQSLSLSFLVSQTSASLVAMTANF
                     FMNNVITYRDQRLRGWEWLGGWFSFVLACSVGALANVGIATYLLDRGTPWSLAGFTGI
                     LVGAVWNYAITQIYTWRAR"
     gene            7277..7795
                     /locus_tag="AC16PP_15740"
     CDS             7277..7795
                     /locus_tag="AC16PP_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR6483134.1"
                     /translation="MSNRFGYVLLLVALVIAALDPLELRAETAVLLDQFGVPGSLAEN
                     AGSVQVVIVVSAKRLRRIKPWERALREQFGDLPVLRVADLPPTSSATHEQVAGKLAKR
                     LPPDLSVLIDLEGRWAGTHGLDTSVPNLLLFAGNGQLIAREAGMFSASGFAEFATRVQ
                     DALDGGAEQAVP"
     gene            complement(7792..9018)
                     /locus_tag="AC16PP_15745"
     CDS             complement(7792..9018)
                     /locus_tag="AC16PP_15745"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6483135.1"
                     /translation="MAIFHAMVLGLLRDRGALLMSFFLPAVFFVIMAEIFTTTSGESL
                     QLNVVVKDELDDEVSGRLLSALQATPTLTLTSDPDLDRDAVRALVKSGAYDIGLIVRA
                     DGEPLDSAGGFGAAPLLIVSDPARGVAIPMLAGQIRRAYFSALPDVAMGNVIGELENQ
                     FLTLTEAQHEAVAEGLLEMRSEAEAGREVGWSFEDLLEQESVAGGESVNLVAYSAGAV
                     AFLFLLFASVHGAVSLLEEREAGILDRVLAGPGGISIMVNGKLIYLVVQGIVQVGVIF
                     MAAWLIYDVDVPANLIEWLVVTLAASLAAAGLAMLLATACSTRRQAQTVANTVILVIS
                     ALGGSMVPRFFMPEALRDLGWLTPNTWALEAYSGIFWRQEPLTHLALPIGLLAGIGVL
                     GWQAALFLARRRAYGP"
     gene            complement(9021..9980)
                     /locus_tag="AC16PP_15750"
     CDS             complement(9021..9980)
                     /locus_tag="AC16PP_15750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6483136.1"
                     /translation="MPTATEQTTPPLLQLDGVRVDLDGRSVLNDVSMSVAGGEVVVLL
                     GPNGAGKTTLLSLACGRRAPDAGRVRLAGHDPRTESSVRRQLGFVPQHVALYQHLSVV
                     ENLSVLGRMMGVARGDLAGRVAEALDWSGLADRARDRVGTLSGGMRRRLNIVASLLHE
                     PALLLLDEPTVGVDVAARERVHELLRRQRSQGLGILLTTHDLPQATELADRVVMLVDG
                     SVRLSGVPGKLIQDTFADKRELTVTLAQPATDPQSRTLRDAGLTPGRGGLTWYGAADN
                     EHADARRWDDRLQEMALAIAEIRVRAPTLEGVYLRLTGEELDL"
     gene            complement(10034..10327)
                     /locus_tag="AC16PP_15755"
     CDS             complement(10034..10327)
                     /locus_tag="AC16PP_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6483137.1"
                     /translation="MEIVNHAREQLGPLTRQLLDAIDTESHPMEASFFAGLNVRLQHM
                     NDEGDLAELFFELSSTAFRGFAFSDHQAALIDRLLRAAEEIAFALSAPDDNAH"
     gene            10601..11017
                     /locus_tag="AC16PP_15760"
     CDS             10601..11017
                     /locus_tag="AC16PP_15760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6483138.1"
                     /translation="MPLRIGFQAPGGPLIAPLWFRYREGSFWSCSPLSSALVKSLESH
                     PEVAFDVSTNEIPYKGVRGRALAECHPASGPDVLEDLLLRYLDGTDNPLAEWLLSRVD
                     GEALIRFHIEWLTSWDFSRRMSGLERIADRDATAEL"
     gene            11017..12411
                     /gene="phrB"
                     /locus_tag="AC16PP_15765"
     CDS             11017..12411
                     /gene="phrB"
                     /locus_tag="AC16PP_15765"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HIR6483139.1"
                     /translation="MRSIVWFRNDLRVTDNPALVEACARGEVTAVTCLCPAQLRAHDV
                     GPMRIAFLLRSLGVLSHDLASIGVSLKIIERPRFADLPEALVDFARQEGATHLEFNDE
                     YPLDERRRDAEVADACAAAGIVVGRHVADCTLSPGTVLTNSGSPYTVFSPFRRRWLER
                     VDRAQLTPMDAPRRRPGKGAGDPVPVTLDGVGAGLGEDLWPAGEAAAQKALKRFLSGP
                     VLDYGDTRDSPALDATSRLSPYLAIGAISVNQCLHAAVEANGGRLDGRSEGISKWITE
                     LIWREFYRHIVALFPHVSQGKSFRRELDALEWRDAPEDYEAWKAGETGYPLVDAAMRQ
                     LNETGWMHNRLRMVTAMFLTKHLLLDWRLGERYFMEKLVDGDFASNNGGWQWSASTGT
                     DAAPYFRILNPTTQGQRFDADGAFTRRMLPVLADVPDKWLFEPKRFDADLGYPDPIVE
                     HRAARARALALFKN"
     gene            complement(12430..13428)
                     /gene="pspF"
                     /locus_tag="AC16PP_15770"
     CDS             complement(12430..13428)
                     /gene="pspF"
                     /locus_tag="AC16PP_15770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HIR6483140.1"
                     /translation="MAAEQDRLLGESPAFLQILEEVSQAAPLEKPVLIVGERGTGKEL
                     IAARLHFLSNRWEGQFLKMNCAAISDSLLESELFGHEAGAFTGATRTHHGRFERAHGG
                     TLFLDELATTSALVQEKLLRVIEYGEFERVGGTKTLHCDVRLVAATNEDLPTRAEEGK
                     FRYDLLDRLAFDVLTLPPLREREEDILLLARHFAITMATELGREYFAGFGSAARDALM
                     THPWPGNVRELKNVVERCVYRSHPDEEVEEVVFDPFDSPYRPASQPSPPQGSTPAGNY
                     DLPLDLKQTVRELEIDLINRALENSKHNQRKAADLLKLTYHQLRGYLKKYELLKSD"
BASE COUNT         2399 a         4251 c         4441 g         2395 t
ORIGIN      
        1 cttcccctgg gtccgtcgga ggtgctcgtg gaggacaccc gctacagcgt gactccgggt
       61 cgggacgccc tttcggacga gagcgccgtg cgaggatacg cgcaagctcg tggctggcag
      121 gtgcaaagcg tcaggggccg ggaggaaggc gtccttccga ttacccttgg cggggacatc
      181 gaagggcttt ggcaggatgc tgccggcatg cccctgatcg gcctgcgtgc cgctctgttc
      241 cacgccacca cggggtactc gtttgccgat gccgtccgca cggcagacgc catcgccacg
      301 tccgcggatc tatcctccga agcgcttggc ggcctcattc gacgccggtc gatccgccac
      361 tggcgtgggc agtcttatct gaggtttctc aatcgcatgc tgtttctcgc tgcgcggccg
      421 ggccagggat atcgcgtgat ggagcgattc tacgggttgc cgcaaggtct gatcgagcgg
      481 ttctacgcag accgtctcac gagtctcgat cgtttgcgga tattgactgg caaacccccg
      541 gttgccattc tgcctgcatt gcgctgcctg tcggagagca gcgtgaagcg atttcactcg
      601 gggatcgagt tttgaacact tcccagcgac cgacagccgc cgtcatcgga tccggctttg
      661 gtgggcttgc gctcgccata cgcctgcaat cggccggctt ccgcacgacc ctcttcgagg
      721 gccgcgaccg tccgggtggc cgggcctacg tctacgagga cgaggggttc acgtttgacg
      781 cggggcccac agtcattacc gacccggact gccttcgtga gctgtttgcg ttgagcggcc
      841 ggtcgctcga ggattacctg gagctgactc ccatcgctcc gttctaccgg ctctgctggg
      901 aggacggcta ctccttcgac tacagcaacg atccggatga gaccgctgcg cagatcggcg
      961 ccaagaaccc agcggacgtt gacggctatc ggcgcttcct cgcttactcg gaggaggtcc
     1021 tcaaggaggg atacgtcaag ctgggcagcg tgccgtttct cgacttcgcc agcatgatca
     1081 aggcggcgcc ccagctggtt cggctgcaga gctatcgcag cgtctacagc atggtgtcgc
     1141 ggttcgtgaa ggacgagcag ctgcggcagg cgttcagctt tcacacgctg ctggtcggcg
     1201 gcaatccgtt caacacctcg tccatctaca cgctgattca ctccctcgag cagcgctggg
     1261 gcgtgcactt tccaaagggt ggaaccggcg ctctggtgca ggggctggtc cgccttttcg
     1321 gcgatcttgg aggcgagctc aagctcaaca cgccggtggc ggagatattg accagcggca
     1381 accgcgtcac gggtgtccgg acggacaacg gctgggaggg acggttcgac gccgtggcca
     1441 gcaacgccga cgtggtgttc acctatgaaa ggctgcttgg ccggacggcg cggggcagag
     1501 cggccgcccg cgcgctgcgg cgcaaaagat tcagcccctc gctgttcgtg gtttactttg
     1561 gcgtcagggg cagctacccc gaactgaagc accatagcat tctcttcggt ccgcgttacc
     1621 gggagcttct caaggaaatt tacgccggtc ccgggctgcc ggacgatttc tcgttgtatc
     1681 tgcacgcgcc cagcgttacc gacccctctc tcgccccgcc aggctgctcg accttctacg
     1741 tgttgtcgcc ggtacccaat ctcaaggcgg ccgacctcga ctggtccgag cttggcccgc
     1801 gttaccggga tcacattctt gcgtacctgg aaaagcacta tctcccgggg ctcggtgagc
     1861 gtctggtcac cacccgcacg ttcacgccgg atgactttca gacgcagctc aacgcgcatc
     1921 ttggctcggc gttttcttta gaacccgtgc tatggcaaag cgcctggttc cgcacgcaca
     1981 accgcgacga cgtgctcaaa ggcctttact ttgccggagc gggcacgcac ccgggcgcgg
     2041 ggatacccgg cgtggtcggc tccgccaagg ccacatcgcg cctgatgctc gatgacttcg
     2101 gccgaacgta tccggacagc gaccggtcgg atcagttcgc caaggagatg gcctgagtga
     2161 gtgacgtttt cgcagccagc gcgtcgatca tctccagggg ctcgaaaagc ttcgctgcgg
     2221 ccgcgcgcct gtttgacccg acgacccggg gtcacgcgat catgctatat gcgtggtgcc
     2281 gccactgcga tgacgagatc gacggccagg atctcggcat cgggcagcgc tctccggcac
     2341 ctccggagca gcgcgcgcgg ctggagcagt tgcggcggga taccgaagcg gccatgcgcg
     2401 gtgaggcggt cgacggaacc ccgttcgtcg cctttcagag agtctttctc gaatgcgaca
     2461 ttccccggaa ctacccgctg gaattgctcg atgggttcgc catggacgtc gagggtcatg
     2521 agtgccgcac gctcagcgac acgctgcgtt actgctatca cgtggcaggg accgtgggcg
     2581 ggatgatggc gacggtcatg gggattcgtg atccagaggt ggtggctcgg gccgtcgacc
     2641 tggggatcgc gtttcaactg accaacattg cccgcgacgt catgcaggac gctgccgacg
     2701 gacgctgcta cctgcccagg caatggctcg ccgacgccgg ggtgcccgag gacaccgggc
     2761 agtttcccga ccaccgcgac gtcatctacg gtcagaccat tcggattctc gacgaggccg
     2821 agcgctacta cgagtcggca agacacggca ttggccgcct taggtttcgc aatgcctggg
     2881 cgatcgccag cgcgcttgcc atctatcgcg acattggtcg ggtcgttcgc cgtgacgggc
     2941 ctgacgtctg gcagcaccgg gcgaccgtcg gcgtaggccg aaagctcgtg ctgcttctta
     3001 aaggagcagc gttagcggga ctgcaggtta cgttcgggcg acgccggacg gcccctatca
     3061 gacgtgggct ctggtcagct tcctgacgcc ccggaggccg ccagggcttt catctccgcc
     3121 cgcaatacgg gaatcggcgg cgcgtagacg aagccgaaag ataccgcgcc atgcttttcg
     3181 gacacggcgt ggtgaaggcg atgcgcgtga acgatacgac gcatgtagcg gctgcgcggc
     3241 gcgtaaccgg tgtcgaggcg tcggtggacg atcacgtcgt gaaagatgaa gtagatcagc
     3301 ccgtaggccg cgacgccgag cccgccccag agcgcggccg gatagccatt gttgccgaga
     3361 tagatgagca cgatggacgg tacggcaaag aacacggcaa accagtcgtt gcgctcgaag
     3421 cgcccctttc ggggctgatg atgcgattcg tgcaggctcc agagaaaccc gtgcatcacg
     3481 tacttgtggg tgacccaggc cacccattcc atgatggcga gagcggtcag gaaacaggcg
     3541 gcgttgacga cgacggtcac ggccgcaacg cgccagcgat ctcggacccc ctcctgctca
     3601 cctgatcggc gtaggcgcga accaccagat ccacgaaaac ggcgaacggc cccgcgtctg
     3661 cgatctggtc ggccagttcg gcaagcgcgt tatcgaggca ggtgatggct tcacgttcgg
     3721 cttcctgaat cgtcatcacg gtggcgaagg tggctttgcc ctcgtcgcga tgagcgtcct
     3781 taccggtgga ctcggatgcg gacaggtcca gaagatcgtc cagaatctgg aaaccgatgc
     3841 cgacccgctc gccgaatttc gtcatcgcgg ccatggtggt ctcgtccacg ccggcgatgc
     3901 gggcgccggc ttccaggcac gccacgaaca gagcgcccgt tttctgatga tggcgcttct
     3961 cgagagcggc ccggctggtc gtactctggg cctcatgcag atcgcgctcc tgcccggcag
     4021 agagcccttg gattccgatc gcgtccgcca ggcggccgac gatgctcgtt cgcacttccg
     4081 gcgcgatccc cggcgcccgg gcgattacgc cgaacgcctc gcttatcagg gaaaccgccg
     4141 agaggatggc gatgtcctcg ccgtgggcga cgtgcgtcgc cggcttgcct cgacgctgaa
     4201 gggcgtcgtc catgcagggc aggtcatcca ggacgagcga ggcggtatgt accatctcga
     4261 ttgcgcaggc tggatccagc gccagatcgg ggtctgcgtc gaatgtcgaa gcagtcagga
     4321 tggtgagcag cggcctgagc cgcttgccgc cgtccagcag gctgtattgg atggcgtgct
     4381 cgatgcgctc gccgccttcg gcagcggacg gcgaaagctc gcgaaggcgc gcttccaccg
     4441 agtggcggat cgcgtccaac ttcgtaatgc tatctggcgc cgcctgctcc aggccaagtc
     4501 tggcttcact caatttggca aaaccttgtc tgggacaggt cattttgcct acatctgccc
     4561 ggcgactcaa aagaaatgga agcgcttgag cctgtcgcgg atctcttcgg gatcaatcag
     4621 cggccgtctt gcgcgaatca gataggcgcc ctgttcccgc gtcatgcagc gattgcagga
     4681 gaagcggtcg aggatggtga tttccgcaaa gagcggttcg agctcctttg ggtggaagcc
     4741 gacgaggagc acgtcctcga gccgggcatt cggctcgtcc gcctgccacc agaggtagta
     4801 gttcatgtgg ccggaatgca cgacgggcag acggtcggcc ttgtccagct ggttgagggc
     4861 gccggcctgg ccgaaatacg cggcgaccac ggcgacttcg ggtctccggg cggagggcag
     4921 gccttcgtag gcccgggtca cccattcggt cagctcaggc catccatgca tctccgcaaa
     4981 gtgcggaaag tatcgcccga tgggtgcgtc atcgtctgcg actttgtcct gttcggcgtc
     5041 ccgaatgaag tttgccacct gctgaagcat ggtgggcgcg agcacggggg cggcaatcgg
     5101 aacggccgcg actccggaga tcaggagaat ggcgagcagg cagctgcggg cgcccgagct
     5161 ggaaagccag cgctgccaga gcacggcgcc caaggcggtg aacagcgcga acagcggcgt
     5221 tgcaaagtag aacttcaccc cggcggcgag aaagaaaacc agggtcgcca ccgctgcgct
     5281 cccgaccacc cgtatgggct tcggagccat cagcgagtaa atcagacccg gaagccagat
     5341 cagcaagaac ggggggttca tggtggcgat gaggccgaag agaggctgat cgagaaccat
     5401 gggctccggt tcgctgacat tgtaagcctg gacgaactcc agcaacggcc accccgaaac
     5461 cgcctgccac atcacgaacg gggccaacat gctcagagct atggccgacc ccgcccagga
     5521 ctccgttcgg tagaacatcc atcgtgcgta cagggcgctg gtgcccagca ggcacaagat
     5581 gaacgcggcg atggcaagct tggtgagaag tgcgaggccg aagatgacgc ctagcaggat
     5641 catgtagcgg ggctgatggt catgcaggta gcgaagcagc acagacaatg caagcagcca
     5701 ccagagctgg tcgaggacgt tcatcgtgaa gatcgactgg accgacataa acgccggagc
     5761 gaagagcacg acgatggccg tggtccattg ggcgaagttg cggccgccga ggatccggca
     5821 cacgtccacc gccaccaacg tggtgacgat gccggcggcg caggcaagcc ctcgcagcca
     5881 gatgagatcg gtgccgccga tccagccgag cagggcgagc agccatggcg ttaggggcgg
     5941 gaaatccaca tagccgaaag ccggccgggc agcagcgtcg aggaggtaga gctcgtcggc
     6001 caggaaaccg tactgggtgc cggcaatcac caggacgaga actttggccg caacgcagaa
     6061 aggccagaca cccgcggctc ggggcgtcgc cggattgatc accgagcgcg ccaggtatag
     6121 atctgcgtga tggcgtagtt ccagacagcg cccaccagaa tccccgtgaa gccggcgagt
     6181 gaccagggag tcccacgatc gagcagatag gtcgcgatgc ccacgttggc cagggcgcca
     6241 acgctgcagg cgagcacgaa ggaaaaccag ccccccagcc attcccagcc tctgaggcgc
     6301 tggtcgcgat aggtgatcac gttgttcatg aaaaagttgg cggtcatggc gaccagcgaa
     6361 gcggacgtct ggctgacgag aaagctcaac gacagggact ggtacagaac gaacagcacg
     6421 gccatgtgca ccagcacgcc aaagccgccg accagcatga aggagacgaa gcgcaccgga
     6481 acgtagcgtc cgaacagctt gtccagcagc agcatcccgt agtcccacaa cgcctggttg
     6541 tccagcttgc tttcaccctg gcttcgggtg cgaaagctgt aggggaactc gcgaaagcgc
     6601 atgggcccgg gtgacgacgc aaacaggtcc atcatgatct tgaacccgat gccggagagc
     6661 tggcgcatgc tcgcttcaaa ggcttcgcgc cggatcatga agaagccgct catgggatct
     6721 cgcagctcgt ctggaacgac catccggctc agccgcgttg cgacccggct cttgcgggcc
     6781 ctgtcgcggg tgaaggcctc ggcgtcacct ccgctgacgt acctgctgcc caccacgatg
     6841 tcgagatcgt ctgcttttag ggcgttcagc attcccggca gcaggctctc gtcgtgctgc
     6901 agatcgccgt ccattaccgc gatgtacggc gcgctgctgg cgagcatgcc ctcgatgcac
     6961 gcggaggaga gaccccgtcg gtccagccgc tgcacgcagc ggacgcgtcc atcgctctga
     7021 gccagccgcc ggacctcacc cgccgtttcg tccggtgagt cgtcgtccac gaagatcacc
     7081 tcccagcgat gatctcccag gcaggcgtcg agccgttcga ccaccatgcc gacattgtcg
     7141 gcttcattga aggtcggcac gacgacagaa agctctacgg ccggtgcccg caaatgttcc
     7201 cccgtggatt cgcgttaggt tcgctcatgg cgaaaggcta aactggcgat gacgaagatc
     7261 ctggattctg gccggtttgt ccaaccgttt tgggtacgtg ctgctactcg tcgcgctggt
     7321 gatcgcggcc ctcgacccgc tggaactccg ggccgagacc gcggtgctgc tcgatcagtt
     7381 cggcgttccg ggatcgctgg cagagaacgc cggctccgtt caggtggtca tcgtcgtttc
     7441 ggccaagcga ttgcgccgca tcaagccctg ggagcgggcc ctgcgggagc agtttggcga
     7501 cctgccggtg ctgcgcgtcg ccgatttgcc acccacgtcg tccgccactc acgagcaggt
     7561 ggcgggcaag ctcgcaaaac gtttgccgcc tgacctttcg gttctcatcg atctcgaggg
     7621 ccgctgggcc ggcacccacg ggctggatac cagcgtcccc aatctgctcc tgttcgccgg
     7681 aaacggacag ctcatcgccc gggaagcagg catgttcagc gcatccggct tcgccgagtt
     7741 cgccacgcgc gtgcaggacg cgttggacgg aggcgcggag caggccgtgc cctaggggcc
     7801 gtaggcgcga cgccgggcga gaaagagcgc ggcctgccaa ccgaggactc cgattcccgc
     7861 cagaaggccg atgggcagcg cgaggtgtgt cagcggttcc tgacgccaga aaatgccgct
     7921 gtaggcttcc agcgcccagg tattgggggt gagccagccg aggtcacgca gcgcttcagg
     7981 catgaagaag cggggcacca tgctcccacc cagggcggaa atgaccagga tgacggtgtt
     8041 cgccactgtc tgagcctgac ggcgggtgct gcaggcagtt gcgagcagca tggctaagcc
     8101 cgcggccgcc agggaggccg ccaacgtgac gaccagccac tcgatcaggt tcgcgggcac
     8161 atcgacgtcg tagatcagcc aggccgccat gaagattacg cccacctgta cgatcccctg
     8221 gacgacgagg taaatcagct tgccgttcac catgatgctg atgccgcctg gacccgcgag
     8281 cacgcgatcg aggataccgg cctcgcgctc ctcgagcagc gaaacagccc catggacgga
     8341 tgcgaagagc aggaagagga acgccaccgc gccggcggaa taggcgacga ggttaacgga
     8401 ttccccgccg gcaacgctct cctgttccag caggtcttcg aaggaccagc ccacctctcg
     8461 gccggcctcg gcctccgaac gcatttccag cagaccctct gctaccgcct cgtgctgggc
     8521 ttcagtgagt gtcaggaact ggttttccag ctccccgatg acgttgccca tggccacgtc
     8581 gggcagagcc gagaaatagg cgcgcctgat ctgacccgcc agcatgggga tggccacgcc
     8641 acgggccgga tcgctgacga tcagaagcgg cgccgctccg aacccgccgg cggagtccag
     8701 cggctctcca tcggcacgga cgatcaggcc gatgtcgtac gcgccggact tcacgagggc
     8761 tctgaccgcg tcccggtcga ggtcagggtc gctggtcaga gtcagtgtgg gggttgcttg
     8821 cagggcggag agaagcctgc cggacacttc atcgtccagc tcgtccttta cgacgacgtt
     8881 gagctgcagg ctctccccgg acgtggtcgt gaagatctcg gccatgatca caaaaaaaac
     8941 cgcgggcagg aaaaaactca tcagcagggc ccctcggtca cgcagaaggc ccaacaccat
     9001 ggcatgaaag atcgccatgc tcacaggtcg agctcctcgc ccgtcaggcg cagatagacg
     9061 ccctctagag tcggggcgcg aacgcggatc tccgcaatag cgagcgccat ctcctgcagt
     9121 cggtcgtccc aacggcgggc atcggcgtgt tcgttatctg cagcaccgta ccaggtgagc
     9181 ccaccgcggc ctggggtcag gcccgcgtcg cgcagcgtcc gggactgggg gtccgttgcc
     9241 ggttgcgcca gggtgaccgt cagctcgcgt ttgtcggcga aggtgtcttg gatcagcttt
     9301 cccggcacgc cggacagacg aacggatcca tcgaccagca tgacgacgcg gtcggcgagc
     9361 tcggtcgcct ggggaagatc gtgagtggtc agcagaattc ccaggccctg ggatctctgc
     9421 cggcgcagca gttcgtgcac gcgctcccgg gcggcaacat ccacgcccac ggtgggctcg
     9481 tcgagaagca gcagagccgg ctcgtggagc aggctggcga cgatgttcag ccgccgacgc
     9541 atcccgcccg acagggttcc gaccctgtcc cttgcgcgat cggcaagccc gctccagtcg
     9601 agggcttcgg cgacccggcc cgccaggtcg ccgcgcgcca cccccatcat ccggcctaga
     9661 acgctgaggt tttccactac ggacaggtgc tggtacagcg cgacgtgctg gggcacgaac
     9721 ccgagctgcc ggcgcaccga actttcggtt cgcgggtcgt ggcccgccag ccgaacccgg
     9781 ccggcatccg gagcgcggcg gccacaggcc agggagagga gggtggtctt gcccgcgccg
     9841 tttggcccca gcagcaccac gacctcgccg cctgccacgc tcatcgaaac gtcgttcagc
     9901 acgctgcgtc cgtccaagtc gacgcgtaca ccgtccagct gcagaagggg aggcgtggtc
     9961 tgttcggtgg cggtgggcac gatgtgggca tcatgccact gaacggtagc aggcgaaatt
    10021 gcggcccggc gctctagtga gcgttgtcgt ccggtgcgct gagggcgaag gcgatctctt
    10081 cggcggcgcg cagcagccgg tctatcaggg cggcctggtg gtcggagaag gcgaaaccgc
    10141 gaaacgccgt gctggaaagc tcgaaaaaga gctcagccag atcaccctcg tcgttcatgt
    10201 gctgaagccg gacgttcagg ccagcgaaaa acgaggcttc catggggtgc gactcggtgt
    10261 cgatggcgtc gagcagttgc cgcgtcagcg gcccgagttg ctcacgggca tggttcacga
    10321 tttccatggt gcgatgctgg cggtggcggg gtgaaattga cgtcaaaacc tcggtgaggg
    10381 ccgtggccgc tctgcgcttg ccggatgccg ccggccgcgc cttgccggcc gcgcctcgcc
    10441 ggccgcgcct cgccggccgc gcactttcac ggctttttta tcggaatcta gataacatcg
    10501 ggttcttgct acggagggtg ggttattgaa ttttcagcct gagaaacact atggggcctg
    10561 gcgagggcgc gaggtggagc gatttttcga atctactcgc gtgcctctgc gcattggctt
    10621 ccaagcgccc ggcgggccgc tgatcgcgcc gttgtggttc cgatatcggg agggttcgtt
    10681 ctggtcctgc agccccctgt cgtcggccct ggtcaagtcg ctcgagagcc atccggaggt
    10741 ggccttcgac gtatccacga acgagatccc ctataaagga gtccgcggtc gggcgttggc
    10801 ggagtgtcac ccggctagcg gccccgacgt cctcgaggat cttctgctga gatatctcga
    10861 cggcacggac aacccgctgg cggaatggct gctgtcgaga gtcgacgggg aagcgctgat
    10921 ccggtttcac attgaatggc tgacgagctg ggacttcagc agacgaatga gcgggctgga
    10981 acggatcgcc gaccgggacg ccacggccga actttgatgc gctcgatcgt ctggtttcgc
    11041 aatgatcttc gcgtcacgga caacccggcg ctggtggagg cgtgcgcgcg gggggaggtc
    11101 acggccgtta cctgtctctg tcccgcccag ctgcgggcgc acgatgtggg cccgatgcgc
    11161 attgccttcc tgctgcggtc cctcggcgtg ctctcgcatg acctggcgtc tatcggcgtt
    11221 tcgctcaaaa tcatcgaacg gccccgcttt gccgacctgc cggaggcgct tgtagacttc
    11281 gcgcggcaag agggcgccac ccacctggag ttcaatgacg agtatccgct ggacgagcgc
    11341 agacgggacg cggaggtcgc cgacgcgtgc gcggccgccg gcatcgtcgt tggtcgccac
    11401 gtggccgatt gcacgctgtc tccaggaacg gtgctgacaa acagcggttc cccctacacg
    11461 gtgttcagcc ccttccggcg ccgctggctg gaacgtgtgg accgggccca gctgacgccg
    11521 atggatgcgc cccgccggcg cccgggcaaa ggcgccggcg atcccgttcc ggtaaccctg
    11581 gacggcgttg gcgccgggtt gggtgaagac ctgtggccgg ccggggaagc ggcggctcag
    11641 aaggcgctga agcgctttct ctccgggccg gtgctcgact acggagatac ccgtgattcc
    11701 ccggcgctcg atgccacgag ccgcctgtcc ccctatctcg ccatcggggc gatatcggtc
    11761 aaccagtgtc tgcacgccgc tgtcgaagcc aacggggggc gtctcgacgg taggtccgag
    11821 gggatatcca agtggatcac cgagctcatc tggcgagagt tctatcgcca catcgtcgcg
    11881 ctgtttcccc atgtctccca agggaagtcg tttcggcgcg agctcgacgc tctcgaatgg
    11941 cgggatgccc cggaggatta tgaggcctgg aaagccggcg agaccggcta tccgctcgta
    12001 gacgctgcca tgcgccagct caacgaaacc ggctggatgc acaaccggtt gcggatggtg
    12061 accgcgatgt ttctcaccaa gcacctgctg ctggactggc gactcggtga acggtacttc
    12121 atggaaaagc tggtcgacgg cgacttcgcc tccaacaacg gcgggtggca gtggagtgcg
    12181 tccacgggca cggatgccgc tccgtatttc cggattctca accctacgac ccagggccag
    12241 cggttcgatg ctgatggcgc cttcacccgg cggatgctgc cggtgctggc cgacgtgccg
    12301 gacaagtggc ttttcgagcc aaaacgcttt gacgccgacc tcggctaccc ggatcccatc
    12361 gtcgagcatc gcgcggcacg cgcgcgggct ctcgcgcttt tcaaaaactg acccgcgccg
    12421 cgcgatcagt cagtcggact tgaggagctc gtacttcttg agatagccgc gaagctggtg
    12481 atacgtgagc ttgagaaggt cggccgcctt gcgctggttg tgcttgctgt tctccagcgc
    12541 gcggttgatc aggtcgatct ccagctcccg cacggtctgt tttaggtcca gtggcaggtc
    12601 gtagtttcct gcgggcgttg agccctgcgg cggggagggc tgtgacgcgg gccggtaggg
    12661 tgagtcgaag ggatcgaaaa cgacctcttc gacctcctcg tcggggtggc tgcggtatac
    12721 gcatcgttcc acgacgttct taagctcccg gacgttgccg ggccatggat gggtcatcag
    12781 ggcatcccgc gctgcgctgc caaagcccgc gaagtactcg cggcccagct cggttgccat
    12841 cgtgattgcg aagtgcctgg caaggagcag gatgtcttcc tcgcgttccc gcaggggggg
    12901 aagcgtgagg acgtcgaacg ccagccgatc gaggaggtcg tagcggaact tgccttcctc
    12961 tgcgcgcgtg ggcagatcct cgttggtggc cgcgaccagc cggacgtcgc aatgcagcgt
    13021 cttggtgccg cctacccgtt cgaactctcc gtactcgatg acgcgcagca gcttttcctg
    13081 aacgagggcg gaggtggtgg caagctcgtc gagaaacagg gtgccgccgt gggctcgctc
    13141 gaaccggccg tgatgggtgc gggttgctcc ggtgaacgcg ccggcctcgt gaccgaaaag
    13201 ttcgctttcc agcagcgagt cgctgatggc cgcacagttc atcttgagaa actgtccctc
    13261 ccaacggttg gacagaaagt gcagccgcgc ggcgatgagt tccttgcccg tgccccgttc
    13321 tcccacgatc aggacgggtt tttccagcgg cgctgcctga gaaacctcct cgaggatctg
    13381 cagaaaggcc ggagattccc cgaggaggcg atcttgctct gctgccatgc tgcgaatctt
    13441 gccaatattt agccgattct gctacgtcct tctagtgccc gggaca
//
LOCUS       DBNHKV010000483         8133 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_849909, whole genome shotgun sequence.
ACCESSION   DBNHKV010000483 DBNHKV010000000
VERSION     DBNHKV010000483.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_849909"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="AC16PP_15775"
     CDS             complement(<1..425)
                     /locus_tag="AC16PP_15775"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6483141.1"
                     /translation="MVSRSAIDDDLYRIGTVATLTGISVERLRAWERRYGLAPTYRSG
                     KTRFYSKQQLQTLQRIKRLIDQGHPISSLAPLSDEQLDERLSAATPRLQALQSATVGL
                     IGPNLLVLEQQQGSDNRLEVRARWANMDAFMSDQTGTER"
     gene            851..2398
                     /locus_tag="AC16PP_15780"
     CDS             851..2398
                     /locus_tag="AC16PP_15780"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0032787 - monocarboxylic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR6483142.1"
                     /translation="MENNLGLFLTKRAFLTPQREGYVDSHSGLRLTFRQLNERSNQLA
                     NSLLQAGVQKEERVGLLLMNSAEFMEAYFALAKIGAVVVPLNWRLVADELEFILKDSG
                     TARLIFDEDFVDTVAELHARGDRTDIRHWLQVEGSQEVAHFAESYEAFRDNGAAEEPP
                     CTACDDDMLYIMYTSGTTGLPKGVVHTHTTAIWAILTIAANAYYEETDRYLGALPMFH
                     VGALTPLAVNVYRGATSVVMRAFDPVLAWELIDSERITSGLMVPAMLNFMLQVGEAKD
                     RYDYSALRWIMTGAAPVPVALTEQYVQMGISILQVYGLTESCGPACLMDSENALRKPD
                     STGKAFFHTDVRVVDAHGNDCAPGEAGEVVVRGKHIMREYWNRPDATTETIVDGWLHT
                     GDVAVMDEEGFVSIQDRIKDMIISGGENVYPAEIEGVLISHPKISEAAVIGQPSERWG
                     ESPLAIVVKADPDLDVAEVMEFCRGKLAGYKQPKGVEFVDEIPRNPSGKILKRILREQ
                     FPGPAPA"
     gene            complement(2444..2827)
                     /locus_tag="AC16PP_15785"
     CDS             complement(2444..2827)
                     /locus_tag="AC16PP_15785"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HIR6483143.1"
                     /translation="MTKPGYTGIKRIRKAAGYSSQGIRAAWQHESAFRQECTVALVLL
                     PCAVLLAETWMQLAVLITVCVLVLIAELLNSAVEAVVDRIGHEHNDLAGQAKDMASAA
                     VALAIFLVPLIWGLVALERFVGYWI"
     gene            complement(2842..3723)
                     /locus_tag="AC16PP_15790"
     CDS             complement(2842..3723)
                     /locus_tag="AC16PP_15790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR6483144.1"
                     /translation="MGHDHNHIHTRDNEVIVGRAFVLIAGFMVVEVIGGLLANSLTLL
                     ADAGHMFLDATALGLSWYALRLSKRSNDHKLSYGYHRFQVLAAFINGLTLMALVVWIL
                     VEAYARLRAPEGMLALPALAVAVVGMVVNLIAFRWLHGAEDNATVRSAALHVIGDLLG
                     SAAAIIAALTVYLTGWLYADPLLAVVIVVILGRGAWRVLKESAHILLEGVPHGINLTE
                     IAETLAARVPGVIEVHHVHAWALTSEKPLVTLHASVDEGTELAGVVSRMKVVLNDDFG
                     IDHSTIQVEHGPCPDDD"
     gene            complement(3838..4983)
                     /locus_tag="AC16PP_15795"
     CDS             complement(3838..4983)
                     /locus_tag="AC16PP_15795"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6483145.1"
                     /translation="MNPVETAAGFAAEIRRRAPEIEQHRRMPGDLANRMAGAGLFRLL
                     VPELYGGLQVEPLAFFETLKETARADGAVGWCQMIGVTTGMMAASLPDEWSRTIYGCN
                     PDTITSGVTAPLGIAVAEGDGLRVSGRWPFGSGCEVSDWICGGCQLMEDGEPRKNRFG
                     SPEIALVFFPADDVTIHDTWHTSGLCGTGSHDIEVKDAEVPAGRWVTMGKRARVDAPL
                     YRFPTLGLLALGVSAVALGIAEHAIEAFTTLARDKVPTGSARPLSARSGAQRDLARAM
                     AAVQSAAALTREAIEDAWGEAHREGSLGLETKARLRLAATNNAWSAAEAVDLLYHAAG
                     GSSIYAHSALQRCFRDVHVATQHLMVAQPIYEVVGKVALGIDPKTLL"
     gene            complement(4980..5459)
                     /locus_tag="AC16PP_15800"
     CDS             complement(4980..5459)
                     /locus_tag="AC16PP_15800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6483146.1"
                     /translation="MAELKVCDVKLAAIRDAGLIARMSRDYIEHGLRWRWRAPQIRAK
                     IRHPETAVIVAEVELGGVRFIGGFASMDFGEDKAALNLLAVHPNLRRRGIGGNLVEWL
                     HKSAGVAGCQHIELQVRAGNVSARRFYGRMGYTEEALINGYYDGKEAAYQMKFRLAQ"
     gene            complement(5537..5986)
                     /locus_tag="AC16PP_15805"
     CDS             complement(5537..5986)
                     /locus_tag="AC16PP_15805"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6483147.1"
                     /translation="MDNQLIAEIQRDCEALSLAYARAIDFRDYDHFVSLFTDDAVLDL
                     GRPLRGRGEIHAAISARPDELRSRHVITNVFVDVVDENTARGIAYLTLYRHIADKPSI
                     PGPGSEPAPTTLPAAVGHYQDRFARGPDGWRFEARSLHLAFRDPAAF"
     gene            6091..6873
                     /locus_tag="AC16PP_15810"
     CDS             6091..6873
                     /locus_tag="AC16PP_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011533963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6483148.1"
                     /translation="MERMSLEFVGKVAVLKFNHPEVMNAVGSQMLLDFADALKDIAHP
                     GNGARCLLLTGEGRGFCAGANLQDGERSERRGGAGDGLRASYHPILLTLRDLNMPMVT
                     AVNGAAAGVGMSFAMMGDIVCASSNAFFLQAFARIGLVPDGGSTFLLPRLVGWGRAME
                     LSMLAERLPAEKAHEWGLVNRLFDDNAALLEGAMAIADRLASGPRSLALIRQAYWNTW
                     HNAFEQQLDLEARLQNQAGASRDFAEGVAAFLEKRDAEFTGE"
     gene            complement(6877..7794)
                     /locus_tag="AC16PP_15815"
     CDS             complement(6877..7794)
                     /locus_tag="AC16PP_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR6483149.1"
                     /translation="MLLITHRDCLLHEMQHRHPERPDRLRAVLQLLESSGLSRDLKHA
                     EALPASQEALRAVHDPDYLRTLSESVPESGLLPVDPDTAMGPSSLRAAALAAGAVADG
                     VRAVLEGDQSTVFCAVRPPGHHAEHDAAMGFCFYNSIALGAVTALEDPRVERVAVLDF
                     DVHHGNGTVDIFKDRPEVLVCSSFQHPFYPHRYADLARPNIVNTPLPAGTAGADFRRA
                     VEAQWLPAIDNHQPQLILVSAGFDAHRLDPLAQLELCEDDFRWITRLIVASAKTHANG
                     RIVSALEGGYDLAALAASAHAHIEELLAA"
     gene            complement(7835..>8133)
                     /locus_tag="AC16PP_15820"
     CDS             complement(7835..>8133)
                     /locus_tag="AC16PP_15820"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR6483150.1"
                     /translation="GGLIEPEKLLRCPVDTLKIDRAVVSALSEDTQAADLAAQICELG
                     ARFNLRVVGVGVETEEQLKLLAEWGCTDAQGLLVSRPVPVTGIQHLMNRLADLN"
BASE COUNT         1415 a         2519 c         2783 g         1416 t
ORIGIN      
        1 atccgctcgg tgccggtctg gtcgctcatg aaagcgtcca tgtttgccca gcgggcgcgg
       61 acttccaggc ggttgtccga gccctgctgt tgctccagca cgaggaggtt cggcccgatc
      121 aggccgaccg tggccgattg cagggcctga agccgggggg tggcagcgga aagtctttcg
      181 tcaagttgct cgtcggaaag cggcgcaagg ctgctgattg ggtgcccctg gtcgatcaga
      241 cgtttgatgc gctgaagggt ctgaagctgc tgcttgctgt agaagcgcgt cttgcccgag
      301 cggtaggtgg gggccagccc gtaacgccgc tcccaggcac gcaggcgctc caccgagatg
      361 ccggtgagcg tggcgaccgt gcctatcctg tagaggtcgt cgtcgatggc ggagcgtgat
      421 accaccctgc agcgtaccag tacaatacct gtacagaatt gtacatgcta acgtcatttt
      481 gtccaggtag ccggtctgac gccggtgcag ggagtgcaga gagtgcagga ggaaggtggc
      541 gtggcgacag cgcattgtgg cgctgtcttc acggtgaggg aggaagggga gagagacacc
      601 gcgccacgcc gatttcattt gcagatccga cgagcgggtc cgcaaagaag gcgcgcattc
      661 tacggatctg caggcagtga tcaagcgcgt ccccgggttt tttgttacga atcttttcac
      721 ctgggcggag actgcgacca gggtgttcac acggacgatc tggttcggga gaatgggctc
      781 gagaggcggt cctgtgagtt cctgcagggg gccttgcgct ttgatgggtt actcgcaagg
      841 ggaggcaacg gtggagaaca atctcggtct ttttctgacg aagcgggcgt ttctgacgcc
      901 ccagcgtgaa gggtacgtcg acagccattc gggcctgcgt ctgacgtttc ggcagctgaa
      961 cgaacgatcc aaccagctcg ccaattcgct cctgcaggcg ggcgttcaga aggaggaacg
     1021 cgtgggtctc ctgctcatga acagcgcgga gttcatggag gcctattttg cgctggccaa
     1081 gataggggcg gtggtggttc ccctcaattg gcgtcttgtc gccgacgagc tggagttcat
     1141 cctcaaggac agcggcacgg cgcggctgat cttcgacgag gatttcgtcg acaccgtcgc
     1201 cgagctccac gcccgcggcg accggaccga catccggcac tggctgcagg tggagggttc
     1261 ccaggaggtt gcccacttcg ctgagagcta cgaggctttc cgggacaacg gggcggccga
     1321 ggagccgccc tgcacggcct gtgacgacga catgctctac atcatgtaca cgtccggaac
     1381 caccggtctg ccgaaagggg tcgtccacac tcacaccacg gccatctggg cgatcctcac
     1441 catcgcggcg aacgcgtact acgaggaaac cgaccgctat ctcggggcgc tgccgatgtt
     1501 tcacgtcggc gcgctgacac ctctggccgt caacgtctat cgcggggcca cttcggttgt
     1561 gatgcgcgcg ttcgatcccg tgctcgcctg ggagctcatc gatagcgaga ggatcacctc
     1621 gggattgatg gttcccgcca tgctgaactt catgctccag gtcggagagg cgaaggaccg
     1681 ctacgactac tcggctctgc gctggatcat gaccggcgcc gcgccggtac ccgtcgccct
     1741 gaccgagcag tacgtgcaga tggggatcag catcctgcag gtctatggcc tcaccgaatc
     1801 ctgcggcccg gcgtgcctga tggattcaga gaacgcgctg cgcaagcccg actccacggg
     1861 taaggccttc ttccacacgg atgtgcgggt ggtcgacgcc cacggcaacg actgcgcgcc
     1921 gggcgaggcc ggcgaagtgg tggtgcgggg caagcacatc atgcgtgagt actggaaccg
     1981 gccggacgcc accacggaga cgatcgtgga cggctggctg cacaccggcg atgttgccgt
     2041 catggatgaa gaggggttcg tttccatcca ggatcggatc aaggacatga tcatctcggg
     2101 cggggagaac gtttaccccg ccgagatcga gggcgtgctg atcagccacc cgaaaatctc
     2161 cgaggcggcc gtgatcggtc agccgagcga gcgttggggc gaatctcccc tggccatcgt
     2221 ggtaaaggct gatcccgatc tggacgttgc cgaggtgatg gagttctgtc gcggcaagct
     2281 tgcagggtac aaacagccaa aaggggtgga gttcgtcgac gagattccgc gcaatcccag
     2341 cggcaagatc ctcaagcgca tcctgcggga gcagtttccc ggtcccgcgc ctgcttgacc
     2401 gtctgtcgag gccgcgggtg gcgggtcctc cgcaggggtc ggatcagatc cagtagccga
     2461 cgaagcgttc gagggccacg aggccccata tcagaggcac caggaagatc gccagcgcga
     2521 cggcggcgga agccatgtcc ttggcctggc cggccagatc gttgtgctcg tggcctatcc
     2581 tgtccaccac cgcttcaacc gccgagttca gcagctccgc gatcagcacc agcacgcaga
     2641 ccgtgatcag cacggccagc tgcatccacg tctccgccag caggacggcg cagggcagca
     2701 gaaccagggc cacggtgcat tcctggcgaa aggcggactc gtgttgccag gcggcgcgga
     2761 tgccctgaga cgagtagccc gctgcctttc tgatccgctt gatcccggtg tagcctggtt
     2821 tggtcatcct agccctcgag cctaatcatc gtccgggcaa ggcccgtgtt caacctgaat
     2881 ggtcgagtga tcgatgccga aatcgtcgtt gagcacgacc ttcatgcgag acaccacccc
     2941 ggcgagctcg gtcccctcgt cgacgcttgc atgcagcgtg accaggggct tctcggaggt
     3001 cagtgcccac gcgtgcacgt ggtgaacctc gatcactccg ggtacccgcg cagcgagggt
     3061 ctcggcgatc tccgtgaggt tgatgccatg cggcacacct tccagaagaa tgtgggccga
     3121 ttccttcagc acccgccagg cgccccggcc gaggatgacg acgatgacga cggcgagcag
     3181 cggatcggcg tagagccaac cggtcagata gacggtcagc gcggctatga tggcggccgc
     3241 cgagccgagc aggtcgccga tgacgtggag cgcggcgctg cgcacggtgg cgttgtcctc
     3301 ggccccgtgc agccagcgga aggcgatcag gttgaccacc atgccgacga cggcaacggc
     3361 cagcgcaggc agagccagca ttccttcagg cgcgcgcagc cgggcgtagg cctcaacgag
     3421 tatccacacc acgagcgcca tcagggtgag cccgttgatg aatgcggcca gcacctgaaa
     3481 gcggtggtag ccgtaggaga gcttgtgatc gttggagcgt ttcgacaacc ggagcgcgta
     3541 ccaggacagg cccagcgcgg tggcgtcgag gaacatgtgc cccgcatcgg cgagcagggt
     3601 cagggagttg gccagcagcc caccgatgac ctcgacgacc atgaagccgg cgatgagcac
     3661 gaaagcgcgg ccaacgatga cctcgttgtc cctggtatga atgtggttgt ggtcgtggcc
     3721 cacgtttacg cgttgtcctg cagccgactc acgaacgccc gtgattatac cgactcaccg
     3781 cgctcggtgg tccgctccgt cccgggcgtc gtgggtcacg tcgccacggg actcggctca
     3841 gagcagcgtc ttcggatcga tgcccagggc gaccttgccg accacctcgt agataggctg
     3901 cgctaccatc agatgctgcg tggcgacgtg cacgtcgcga aaacaccgct gcagcgcgct
     3961 gtgcgcgtaa atcgacgatc ctcccgcggc atggtagagg aggtccaccg cctcggccgc
     4021 cgaccaggcg ttgttggtgg cggccagcct gaggcgggcc ttggtttcca ggccgaggga
     4081 tccttcgcgg tgggcttccc cccaggcgtc ttcgatggcc tcgcgggtga gcgccgccgc
     4141 tgactggacg gcggccatcg ctctggccag gtcgcgctgg gccccagacc gggcggaaag
     4201 aggccgggcg ctgccggtgg ggaccttgtc ccgggcgagc gtggtgaaag cctcgatggc
     4261 gtgctctgcg atgcccagtg caaccgcgga cacgccaagg gcgagcaagc ccagcgtagg
     4321 aaatcggtag aggggcgcgt ccacccgcgc gcgcttgccc atggtgaccc atcgccccgc
     4381 gggaacctcg gcgtccttta cctcgatgtc gtggctgccg gtcccgcata agcccgacgt
     4441 gtgccaggta tcgtgaatcg tcacgtcgtc tgcgggaaag aaaaccaggg cgatctcggg
     4501 cgagccgaac cggttcttgc gcggctcgcc atcttccatg agctggcagc cgccgcagat
     4561 ccaatccgag acctcgcacc cgctgccgaa gggccaacgg ccgctgaccc tgagaccgtc
     4621 gccttcggca acggcgatgc ccagcggggc ggtgacgccg gaagtgatgg tatcggggtt
     4681 gcaaccgtag atcgtgcgag accattcgtc gggcagcgag gcggccatca tcccggtggt
     4741 cacgccgatc atctggcacc agccgaccgc cccgtcggcg cgggccgtct ccttgagggt
     4801 ttcgaagaaa gctagaggct cgacctgcag gccgccgtag agctccggga cgagcaggcg
     4861 gaacagtccc gcaccagcca tgcgatttgc gaggtcgccg ggcatccggc gatgctgctc
     4921 gatctccgga gcacggcggc ggatttccgc agcgaagcct gcggcggtct cgaccgggtt
     4981 cattgggcca ggcggaactt catctggtaa gcggcctcct tgccgtcgta gtagccgttg
     5041 atgagggctt cctcggtgta gcccattcga ccatagaaac gacgcgcaga cacgttgccc
     5101 gctctgacct gcagctcgat gtgctggcag ccggccacgc ccgcgctctt gtgcagccac
     5161 tcgacgaggt ttccaccgat gccgcgccgt ctcaaattcg ggtgcacggc aagcaggttc
     5221 aatgcggcct tgtcctcgcc gaaatccatc gaggcaaaac cgccgatgaa gcgaacgccg
     5281 ccgagctcca cctccgcgac gatgacggcc gtttcgggat gccggatctt ggcgcggatc
     5341 tgcggcgcgc gccagcgcca gcggagcccg tgctcgatgt agtcccggga catgcgcgcg
     5401 atcagccccg cgtctctgat tgcggccagc ttgacatcgc agactttcaa ttcggccatt
     5461 gaccgattat tgccaatccc ccatcccgag gggaacgggt ccgggcgccg aggttgcaaa
     5521 cgacttgtag aaccggtcag aaggcagccg ggtcgcggaa cgccagatgc aggctgcgcg
     5581 cctcgaaccg ccatccgtcc ggcccgcggg cgaaccggtc ctggtagtgg ccgacggcgg
     5641 cgggaagcgt ggtcggcgcg ggttcgcttc cgggacccgg tatcgacggt ttgtcggcaa
     5701 tgtggcgata gagggtcaga taagcgatgc cgcgggcagt gttttcatcg acgacgtcta
     5761 cgaacacgtt ggttatgacg tgacgcgagc gcagctcgtc tggccgcgcc gagatggcgg
     5821 cgtgaatctc cccgcgcccg cgcagcgggc ggccgaggtc gagcacggcg tcgtcggtga
     5881 aaagggagac gaaatggtcg tagtcccgga agtcgatggc gcgggcgtat gccagggaga
     5941 gcgcttcgca gtcgcgctgg atttccgcga tcagctgatt gtccacgtcg ggtttctccc
     6001 tcctggtttg agcctggcca tcgggttggt tatcgttccc gatccgtcgc agatcgacaa
     6061 gtcggtcgcg ctttcagatt gggggaacca atggaacgaa tgtcgctgga gttcgtgggc
     6121 aaggtggccg tgctcaaatt caatcatccc gaggtaatga atgcggtcgg cagccagatg
     6181 ctgctggatt tcgccgacgc gttgaaggac atcgctcatc cgggcaatgg cgcccgctgc
     6241 ctgctgctca ccggcgaggg gcgcggcttc tgcgccggcg caaaccttca ggatggcgag
     6301 cgctccgagc gccgcggcgg cgccggtgac ggcctgcgtg ccagctatca tccgatactt
     6361 ctaaccttgc gggatctcaa catgccgatg gtcaccgccg tcaacggggc cgctgccggc
     6421 gtcggcatga gctttgccat gatgggcgat atcgtctgcg catcttcgaa cgccttcttt
     6481 ctgcaggcgt tcgcgcgaat cggtctggtg cccgacggtg gctcgacctt cctgctgccc
     6541 cgcctggtgg gatggggccg cgccatggag ctctccatgc tggccgagcg gctgccggcg
     6601 gagaaagccc acgagtgggg gctggtgaac cgtttgttcg acgacaacgc ggccctgctc
     6661 gaaggtgcca tggccatcgc cgaccggttg gccagcgggc cgagatcgct tgccctgatt
     6721 cgccaggctt actggaacac gtggcacaac gcgtttgagc agcagctcga tctcgaagcc
     6781 agactgcaga atcaggccgg cgcgtcgaga gacttcgccg agggcgtggc cgcgttctta
     6841 gagaagcgcg acgcggagtt caccggcgag taacgactac gcggcgagca gctcctcgat
     6901 gtgcgcatga gccgatgcgg ccagcgcggc gaggtcgtaa ccaccttcta aagcggagac
     6961 gatgcgtccg ttggcatggg tcttcgcgct ggcgacgatg agccgggtga tccagcggaa
     7021 atcgtcctcg cacagctcga gctgagccag ggggtcgagg cggtgggcgt cgaaccccgc
     7081 tgagaccagt atcagctggg gctggtggtt gtcgatggcg ggaagccact gcgcctccac
     7141 ggcccggcga aagtcggcgc ctgcggtgcc tgcgggcagg ggcgtgttga cgatgttggg
     7201 gcgcgccagg tctgcgtagc ggtgggggta gaacgggtgc tggaagctcg agcagaccag
     7261 cacctcgggc cggtccttga atatgtccac cgtgccgtta ccgtggtgga cgtcgaagtc
     7321 caggacggcg acgcgctcta cgcgggggtc ttcgagggcg gtgacggccc ccagggcgat
     7381 gctgttgtag aagcagaaac ccatggccgc gtcgtgctcg gcgtgatgac ctggagggcg
     7441 aacggcacag aacactgtgg attggtctcc ctcgaggacg gcgcgaacgc cgtcggcaac
     7501 cgcgccggcg gccagggcgg ccgcccgcag gctggatggc cccatggccg tgtccggatc
     7561 aacgggcagc agtccggact cgggcaccga ttcggacagc gtgcgcaggt aatccggatc
     7621 gtgcacggct cgcagcgctt cctggctcgc cggcaaggcc tctgcatgct tcaggtcccg
     7681 ggacagaccg ctggactcga gcagttgcag caccgcgcga agacgatcgg gtcgctcggg
     7741 atgccggtgc tgcatctcgt gcagcaggca gtcccggtga gtgatcagca gcatcggccg
     7801 atggtagcag ggtccccgga aggaagcagc ggggtcagtt taagtcggcg aggcgattca
     7861 ttaggtgctg gattccggtt accggtaccg gacgagagac gagcagcccc tgggcgtccg
     7921 tgcagcccca ctcggcgagc aatttgagct gctcctcggt ctcgacgcca accccgacga
     7981 cgcgcaggtt gaatcgcgcc cccaactcgc agatctgcgc ggcgagatcg gccgcctggg
     8041 tatcctcgga caacgcgctc accacggctc ggtcgatctt gagcgtgtcc accgggcagc
     8101 gcagaagttt ctcgggctcg atgagcccgc cgc
//
LOCUS       DBNHKV010000484         3725 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_67721, whole genome shotgun sequence.
ACCESSION   DBNHKV010000484 DBNHKV010000000
VERSION     DBNHKV010000484.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_67721"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="AC16PP_15825"
     CDS             complement(<1..424)
                     /locus_tag="AC16PP_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HIR6483151.1"
                     /translation="MSEVTTSVRITTVISGPVRRSDRIDSLDVMRGVALLGILLMNII
                     AFGLPFATYYNPSVAGIDNPANLWAYIVNWLFFEGTMRALFSMLFGAGMVLMTTRAEE
                     RGAGIEAADVYYRRLLWLILFGVIDAYLLLWVGDILFLY"
     gene            complement(517..900)
                     /locus_tag="AC16PP_15830"
     CDS             complement(517..900)
                     /locus_tag="AC16PP_15830"
                     /inference="COORDINATES: protein motif:HMM:NF024030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HIR6483152.1"
                     /translation="MLELNPESVAQIAEKAREFQSEEILLPPEPPPVPVDDTAFEYAA
                     EWHGDPGYNDLKDAIEDLEPDQQSVLVALMWLGRGDYGIEEWNEALAYAEEVHTDATA
                     DYLIATPLLADYLLGGLEMHGYEPE"
     gene            1033..2172
                     /locus_tag="AC16PP_15835"
     CDS             1033..2172
                     /locus_tag="AC16PP_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR6483153.1"
                     /translation="MTENLRGLRLTSTVKSEGVLELALVESEVRSPGPDEVVVRIEAA
                     PINPSDLGLLFGLADMTTAEFSGSPERPVVQARIPGNFMKHMAGRLDEPMAVGNEGAG
                     VVVAAGASEAAQALMGRTVAVLGGAMYAQYRVLNVSMCLPLGEDTSPREGAACFVNPL
                     TALGMVETMRMEGHSALVHTAAASNLGQMLQKICSADGVQLVNIVRKPEQVEILRGIG
                     AAHVVNSAAENFKDDLTEALATTGATLGFDAIGGGRLASDILACMEAAANRAATEYSR
                     YGSTTHKQVYIYGGLDLGPTQLHRSFGMYWGVGGWLLTPFLQKIGPEATQRLKERVAD
                     EVKTTFASHYAEEVSLAGALEPEAISAYGQKATGQKYLINPNAGL"
     gene            complement(2191..2637)
                     /locus_tag="AC16PP_15840"
     CDS             complement(2191..2637)
                     /locus_tag="AC16PP_15840"
                     /inference="COORDINATES: protein motif:HMM:NF025050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6483154.1"
                     /translation="MTMPDNTSITQMGYVVADLDAAMERWKTTVGAGPFERLNHVEVV
                     DGLYRGTPTNVDISVAIAEVGGIQLELIEQHNDVPSCYRDLFAAGEEGLHHVAVQPED
                     FDAEVARYEALGCPQAFGGLYEGARFVYMDTSGSLGIMVELVERRE"
     gene            2694..>3725
                     /locus_tag="AC16PP_15845"
     CDS             2694..>3725
                     /locus_tag="AC16PP_15845"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6483155.1"
                     /translation="MTAGSGTLHSDMAWPRQGYAWYVVIILFVCSIFSFLDRQVIALM
                     VDDIKLDLGLNDFEIGLLQGPPFGIFYALMSIPIALAADRYSRRNIIIAGVAFWSAAT
                     AACGLAGNFWHLFLARVGVGSGEACLSPCAYSMISDYFRRQLLPLAMSIFTMGNLTGV
                     GLAMIIGGAVIGYAKSLDEIVIWGLGDVAPWQFAFIVIGLPGLLLAALIFTVREPLRR
                     GLSAPTAETDATSLRAFGGFVRQHWRTFATLFGSFTMLVLVAFGNFAWVPTFFIRTFG
                     WSEAYAGTIYGSIVAIFGTGGALFGGWFASYLMRRGYVDAPYRATLICSLPLAPLAVL
                     VFVVIPDGHW"
BASE COUNT          603 a         1203 c         1230 g          689 t
ORIGIN      
        1 cgtacaggaa gagaatgtcg ccgacccaaa gaagcaggta ggcgtcgatg acgccgaaga
       61 ggatcagcca cagcaggcgc cgatagtaca cgtcggccgc ttcgatgccg gcgccgcgct
      121 cctcggcccg ggtggtcatc agcaccatcc ccgcgccgaa cagcatggag aacagcgcgc
      181 gcatcgtgcc ttcgaagaac agccagttga cgatgtaggc ccagaggttg gcggggttgt
      241 cgatcccggc aacgctgggg ttgtagtagg tggcgaacgg caatccgaac gcgatgatgt
      301 tcatgagcag gatgcccaga agcgccacgc cgcgcatgac gtccagcgag tcgatccggt
      361 cgctgcgccg aaccgggccg gaaatcacgg tcgttatcct gacggaggtg gtgacctcgc
      421 tcatgcccct ctcctgaaac ctcccgcagc ctggagtttc ggcggattga acggctttgg
      481 caaccgggtg aacccggccg tcgtgggagc gccgcgctat tcgggctcgt agccgtgcat
      541 ctccaggcct cccagcaggt agtcggccag aagaggggtt gcgatcaggt agtcggcggt
      601 cgcgtcggtg tgcacctcct cggcgtaggc gagcgcttcg ttccactcct cgatgccgta
      661 gtcgccccgg ccgagccaca tcagcgccac cagcaccgac tgctggtcgg gttcgagatc
      721 ctcgatggcg tccttcagat cgttgtagcc cggatcgccg tgccactcgg ccgcatactc
      781 gaaagccgtg tcgtccacgg gaacgggggg cggctccggg ggcagcagga tctcctcgga
      841 ttgaaactcc cgcgctttct cagcgatctg cgccacgctc tcgggattga gttcgagcat
      901 accgcctgcc tcctcgtttc attctgtcac caaccagcgc ttattcttgc cgttctacgg
      961 agcaagcaaa tcaggggttg tacataggcc ataacggtgt acggtctccg ggcgaagaaa
     1021 ggaggacgca tcatgacgga gaatctgcga ggcctgaggc ttacctcgac ggtaaagagt
     1081 gagggcgtgc tcgagctggc cctggttgaa tcagaggtgc gaagccccgg ccctgacgag
     1141 gtggtggttc gcatagaggc agcgcccatc aacccgtccg accttggcct gctcttcggc
     1201 ctggcggaca tgaccacggc ggaattctcg ggctcgccgg agcgtccggt ggtgcaggca
     1261 cggatccccg ggaatttcat gaagcacatg gccggtcggc tggacgagcc catggcggtt
     1321 ggcaatgagg gtgcgggcgt cgtggtggcg gcgggcgcct ccgaggctgc tcaagcgctc
     1381 atgggccgga ccgtggccgt gctggggggc gccatgtatg cccagtatcg ggtgctgaac
     1441 gtgtccatgt gcctgcccct tggcgaggac acttccccgc gtgagggggc cgcctgcttc
     1501 gtgaacccgc tgacggctct ggggatggtg gagaccatgc gcatggaggg tcactcagct
     1561 ctcgttcata cggccgcggc ctccaacctc ggccagatgc tgcagaagat ctgctcagcc
     1621 gacggcgtgc aactggtcaa catcgtccgc aagcccgagc aggtggagat cctgcggggg
     1681 attggtgccg ctcatgtggt gaactccgcg gccgagaact tcaaggacga tctcaccgag
     1741 gcgctggcga ccacgggggc gaccctggga ttcgatgcca tcggcggggg ccggctggcc
     1801 agcgacatac tcgcctgcat ggaagccgcc gcgaaccggg cggccactga gtacagtcgc
     1861 tacggatcga cgactcacaa gcaggtctac atctacggcg gtctcgatct cggccccacg
     1921 cagctgcatc gctccttcgg gatgtactgg ggcgtggggg gctggctcct cacgccgttc
     1981 ctgcagaaga tcggtcccga ggcgacgcag cggctgaagg agcgggtggc ggatgaggtg
     2041 aagaccacgt tcgcgagcca ctatgccgag gaggtctcgc tggccggcgc cctggaaccc
     2101 gaagcgatct ccgcctacgg gcagaaagcc acgggccaga aatatctgat caatcccaac
     2161 gccgggctct gaaccacccc ggctggcgcg tcactcccgc ctctccacga gctcgaccat
     2221 gatgcccagc gagccggacg tgtccatgta gacgaagcgg gcgccttcgt agaggccgcc
     2281 gaaggcctgg gggcagccga gcgcctcgta gcgcgccact tccgcgtcga aatcctcggg
     2341 ctgcacggcc acgtggtgca ggccctcctc gcccgcggca aagagatccc ggtagcagga
     2401 gggcacgtcg ttgtgctgct cgatgagctc gagctggatg ccgcccacct cggcgatggc
     2461 cacggagatg tccacgttcg tgggcgttcc ccggtacagg ccgtcgacga cctcgacgtg
     2521 attcaggcgc tcgaacgggc ctgcgccgac ggtggttttc cagcgctcca tggcagcgtc
     2581 gagatcggcg accacgtagc ccatctgggt aatgctggtg ttatctggca tagtcattgg
     2641 ctgtcccctg cgcagcgaga gttaaggata accttgtccg gacccccatc accatgaccg
     2701 ccggctccgg aacgctgcac tccgacatgg cctggccgcg gcagggctac gcctggtacg
     2761 tggtcatcat cctgttcgtc tgctcgatat tctccttcct cgatcggcag gtcatcgcgc
     2821 tgatggtgga cgacatcaag ctggacctgg gcttaaacga tttcgagatc gggttgctgc
     2881 aggggccgcc gttcggcatt ttctatgcgc tgatgtccat tcccattgcg ctggccgccg
     2941 accgctacag ccggcgcaac atcatcatcg ccggcgtggc gttctggagc gcggccaccg
     3001 ccgcctgcgg ccttgccggc aacttctggc atctgtttct cgcccgggtc ggcgtgggca
     3061 gcggagaagc gtgcctgtca ccgtgcgcgt actccatgat cagcgactat ttccggcgtc
     3121 agcttctgcc cctggccatg agcatcttca ccatgggcaa cctcaccggc gtcgggctcg
     3181 ccatgatcat cggcggggcg gtgatcggct atgccaagag cctggacgag atcgtgatct
     3241 ggggtctcgg cgacgtggca ccctggcagt ttgccttcat cgtcatcggc ctgccggggc
     3301 tgctgctcgc tgcgctcatc ttcaccgtcc gcgagccgct gcgccgcggc ctgtcggcgc
     3361 cgacggcgga gacggacgcc acgtcgctca gggccttcgg agggttcgtt cgccagcact
     3421 ggcgcacgtt cgccaccctg ttcggcagct tcaccatgct ggtgctggtg gccttcggca
     3481 acttcgcctg ggtccccacg ttcttcatcc gcaccttcgg ctggtccgaa gcctacgccg
     3541 ggacgatcta cggctccatc gtggccatct tcgggacggg cggcgcgctg ttcggcggct
     3601 ggtttgccag ctacctgatg cggcgcggct acgttgacgc gccttaccgg gcgaccctca
     3661 tctgctcgct gcctctggcg ccgctggcgg tgctggtatt cgtggtcatt cccgacgggc
     3721 actgg
//
LOCUS       DBNHKV010000485         6535 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_1841560, whole genome shotgun sequence.
ACCESSION   DBNHKV010000485 DBNHKV010000000
VERSION     DBNHKV010000485.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6535)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_1841560"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="AC16PP_15850"
     CDS             complement(<1..302)
                     /locus_tag="AC16PP_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6483156.1"
                     /translation="MYDHLIRNATIVDGLGGKPCTGDIAIKDGVIAAVGEVSGNARET
                     LNAEGAYATPGWVDVHTHYDGQVSWDDTIEPSFSQGVTSIVMGNCGVGFAPCRPGD"
     gene            421..795
                     /locus_tag="AC16PP_15855"
     CDS             421..795
                     /locus_tag="AC16PP_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6483157.1"
                     /translation="MTSSIQTGAVLVVFGLAIGAASASAEVCDVDQVLQRLQSEVLGA
                     LSEEQQTRAREVLGEFCGGMISESPRRVVSTADPTEEAASKAPGELQEAEESEGTLKV
                     LGVEFQKADEDSRGHERLKKKR"
     gene            806..2140
                     /locus_tag="AC16PP_15860"
     CDS             806..2140
                     /locus_tag="AC16PP_15860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR6483158.1"
                     /translation="MSNQRAAEFWARTRDVAGTWGLALALLLAGLIVAYQFVGPPPPK
                     RFVLATGESSGAYYYFGERFAQVLARDGVVVELRATGGSVDNLDLVAREGGADLAFVQ
                     GGIASSAPVDNLVTMGSLYLEPLWLFLGPGVVLDDLGDLKGRRLAVGVEGSGTRAVVM
                     QLLEANGVDAANSKLLGVASAALPDALAGGEIDAAFLIGAPQSSTVGKLLRLPGVRLH
                     DMERVEAYTRRNAFLSSVVLPRGVLDLEADLPAKDTETVAVGAMLVARDDFHPALTDL
                     LLIAAAEIHGGHSILADAGTFPTPRFVDLPLSEEAERHYRHGAPFLMRYLPFWAATLV
                     DRLWIMLVPLIGLSLPLARLVPPAYRWQIRRRLLRIYTKLHALDPLRTPIADDEDVAR
                     RLEELDRLDVDSTIEAVPKGYADDLYQLRRDIDLVRRRLGAARSAGRGQQND"
     gene            2207..2857
                     /locus_tag="AC16PP_15865"
     CDS             2207..2857
                     /locus_tag="AC16PP_15865"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6483159.1"
                     /translation="MVAHSKSVTYTRAPAEKRERLLAAARELFAERGFEDTSTQQIAK
                     TAGVSEGILFHHFGSKRGLLERIAEDFVAAGVQAAMPDTPGQLSEEMVVRGAFDFADA
                     HPALYRLLEQMSRELGGSSGASRSAVIVAAIEKSLRAGMAQGIVREGDAGIMAELQFA
                     LVDAAYKAWRDGGDPDRREDYIREAVGCMKAMLAPSGAEERPPLADATKLSRGDAT"
     gene            2854..4173
                     /locus_tag="AC16PP_15870"
     CDS             2854..4173
                     /locus_tag="AC16PP_15870"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF015043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006720 - isoprenoid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HIR6483160.1"
                     /translation="MSEAVSNEAPFWLSGNFAPTFDEVTETRLEVTGTIPPELKGRYL
                     RNGANPESGVTPHWFLGNGMIHGVELNDGRANWYRNRYVRTPLLSDGDHSDGVDERAK
                     SLANTHIIGHAGRILALEEGHWPFELTPELDTVGPYTFGGRLETGMTAHPKICAETGE
                     LLFFAYAATPPFMTYHRATADGELVQSEVIEVKGATMVHDFNITRNYVIFMDLPVVWN
                     FENAASVGLPLAWSDEYGARLGVMPRNGSNADVVWYDIDPCYVYHPLNAYEDGDDIVI
                     DVCRMAHHMKIGAPEAPPMLHRWTIHQSAGTVSEAQLDERSVDFPRVPDALVGQKHRY
                     GYTAEFGGGLPAAVAFRKYDLATGTSEAHVLADGRAGGEPVFVPARGGTSEDDGYLMS
                     FVYDPADNRSELVIIDASNVTADPVARVHLPLRVPAGFHGSWVADAQ"
     gene            4214..5212
                     /locus_tag="AC16PP_15875"
     CDS             4214..5212
                     /locus_tag="AC16PP_15875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR6483161.1"
                     /translation="MINKKVVLASRPEGQPKESDFKVIEEELAELTEGQVLLATEHLS
                     IDAFIRTMLDSQEGLHGTVAIDTPVMALGVARVVDSRSPDLNQGDAVFGPMGAQTHLV
                     APGGMYRKLEEGDVPPRSYLGALGMTTGLTAYAGMLKVGQVAEGDTVVVSAAAGAVGT
                     IACQLAKIAGAKVIGIAGGPAKVAYLIDEIGCDGAIDYKNDDVAEKLRALAPAGVNVF
                     FDNVGGELLDIVLDQIAQGARVVICGAISQYNHMEEVRGPRLYLRIPERNASMLGFTV
                     DRFAADFPEMEADISRWMAEGKLKMPEHVVEGIEQFPKALTMLFTGGHMGKLLVAP"
     gene            5281..>6535
                     /locus_tag="AC16PP_15880"
     CDS             5281..>6535
                     /locus_tag="AC16PP_15880"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6483162.1"
                     /translation="MRGIFLALVAVCLQVPVLAVAAEIDFTRHLVRVESAAGQHPGPD
                     VGPARPFFGVAGPARVIVTARSGEPVVTVRLNGVDVLDGQALRQTTSLEIPVELAEAN
                     TIAVGLDGRDGAAASVRVKQRADVALNVISWVHFNTNVSDFNEARAFYGVLGFETMSG
                     FPDTNTLAMAHALGIQTPTAYDGSQGEEAGGYLLHGELVGPDGFWGGVIDLIEFTIPR
                     NEEPPYPGLNHLGMARALMHTGDIAAAYDQLVGAGVNFLSPPATRSDGTRFAVFTDPD
                     GTFYELVELEGPGGEASEKPVIGLGRVNVNVSDFERSEAWYRMFGYVATRKLPPTDSA
                     EVAAAMGFDAPYRIDGAVLSHEVDGSQIELVQWLSPFDPESAYPPPVSHLGIHRMALL
                     TGDIEADVAALKAQGVKFISGITPCC"
BASE COUNT         1119 a         2066 c         2224 g         1126 t
ORIGIN      
        1 tcgtcccccg ggcggcacgg cgcgaagccg acgccgcagt tgcccatgac gatgctggtc
       61 acaccctggc tgaacgacgg ctcgatggta tcgtcccagc tgacctgacc gtcgtagtgg
      121 gtatgcacgt ccacccagcc gggagtggcg taggcgccct ccgcgttcag ggtctccctg
      181 gcgtttccgg agacttcgcc caccgcggcg atgacgccat ccttgatggc aatgtcgccc
      241 gtgcatggct ttccgccgag accgtcgacg atggtggcgt tcctgatcag gtggtcgtac
      301 atggtggctc ctcgagcaat accgtgcccg tcgtggtgac tgtgggcgta acgaagaaaa
      361 gggtggcctc tcgtgcacga acggcagcac gtctgagccg cggtggtgca taatgccgcc
      421 atgaccagct cgattcaaac cggggccgtt ctcgtcgtct tcgggctcgc gatcggcgcg
      481 gcttcggcaa gcgcagaggt ttgcgatgtc gatcaggtac tacagcgcct gcaatccgaa
      541 gtgctgggcg cgctgtcgga agaacagcag acgcgagccc gcgaggtgct tggggaattc
      601 tgcggcggca tgatttcgga aagccccaga cgggtggtca gcacggccga cccgaccgaa
      661 gaggctgcct ccaaagcgcc cggggagttg caggaagccg aggagtcgga ggggacgttg
      721 aaggtgctcg gggtggaatt tcagaaggcc gacgaggatt cgcgcggcca tgagcgcctg
      781 aagaagaagc gttgaccgtt cgagcatgtc caatcaacgt gcagctgaat tctgggcccg
      841 gaccagggac gtagcgggaa cctgggggct cgcgctcgcg ctcctgctgg cggggctcat
      901 cgtcgcctat cagttcgtcg ggccgccgcc gccgaaacgg ttcgttctcg caaccgggga
      961 gtcgtccggc gcttactact atttcggtga gcggtttgcc caggttctgg cccgggacgg
     1021 ggtggtggtc gaactgcgcg ccaccggtgg cagcgtggac aatctcgatc tggtggccag
     1081 ggaagggggg gccgacctcg ctttcgtgca ggggggcatc gcgtcgtctg cccccgtgga
     1141 caaccttgtc actatgggca gcctgtatct ggaacccctc tggctgttcc ttggaccggg
     1201 cgtggtgctc gacgacctgg gcgatctgaa gggccggcgg ttggcggtgg gtgtggaagg
     1261 cagcggaaca cgcgccgtcg tgatgcagct gctcgaggcc aacggggtgg acgcggcgaa
     1321 ctcgaagctt ctgggggtgg cgtcagccgc gcttcccgac gcgctggctg ggggcgagat
     1381 cgacgcggcg tttctcatcg gcgcgccgca atcctccacg gtcgggaagc ttctccgcct
     1441 gccgggagtt cgtctgcacg acatggaacg ggtcgaggct tacacgcggc gcaacgcctt
     1501 cctctcttca gtcgtcctgc ccaggggcgt tctggatctg gaagcggacc tgccggcgaa
     1561 ggacaccgag acggttgccg tgggggccat gctcgtggcc cgtgatgatt tccatccggc
     1621 gctcacggac cttctgctca tcgcggccgc cgagatacac ggtggtcaca gcattctcgc
     1681 ggatgcgggc acgtttccca cgccgcgttt cgtcgacctg ccgctcagcg aggaggcgga
     1741 gaggcactac cggcacgggg cgccgtttct catgcgctac ctgccgttct gggcggccac
     1801 gctggttgac cggctctgga tcatgctcgt cccgctgatc gggttgagcc tgccgctggc
     1861 gcggctggtg ccccctgcgt atcgatggca gatccgtcgg cggctgctgc gcatctacac
     1921 gaagctgcac gccctggatc cgcttcgaac gcccatcgcc gacgatgagg acgtggcccg
     1981 tcggctggag gagctcgatc ggctggacgt ggactcgact atcgaggcgg ttccgaaggg
     2041 ctatgccgac gacctctacc agcttcgccg cgacatcgac ctcgtccggc ggcgccttgg
     2101 ggcggctcgc tcggcgggcc gcggccagca gaacgactga aaacctgcat gccgcaaatt
     2161 gggggtttac ttgttgagta acactttgta aagtgttact caataaatgg tcgcgcactc
     2221 taaatcggtt acctacacca gggcccctgc ggaaaagcgg gagcgattgc tggcagccgc
     2281 ccgggagctg tttgccgagc gcgggttcga agacacttcg actcagcaga tcgccaagac
     2341 ggcgggggtc tcggaaggca tcctctttca ccacttcggt tcgaaacgag ggctgctcga
     2401 gcgcatcgca gaggatttcg tggccgccgg cgttcaggcg gccatgcccg atacgccggg
     2461 ccagctcagc gaggagatgg tggtgcgggg ggcgttcgat tttgccgatg cccatccggc
     2521 actgtaccgg ctgctcgagc agatgagccg agaactcggc gggtcgagtg gcgccagccg
     2581 cagcgcggtc atcgtcgcgg ccatcgagaa gagcctgcgg gctggaatgg ctcagggcat
     2641 cgtccgggaa ggggatgccg gaatcatggc cgagctgcag ttcgccctgg tggacgcggc
     2701 ctacaaggca tggcgggacg gcggtgatcc ggaccgcaga gaagattaca tccgcgaagc
     2761 ggtcggctgc atgaaagcca tgctggcccc gagcggagcc gaggaacgac cgccgctggc
     2821 ggacgcaacg aaactcagtc gaggagacgc aacatgagcg aagcagtcag caacgaagca
     2881 cccttttggc tctcgggcaa tttcgccccg acctttgacg aggtgaccga gaccaggctc
     2941 gaggtgaccg ggaccatccc cccggagctc aagggacgtt acctgcgcaa cggcgccaac
     3001 ccggaatccg gcgtcacgcc gcactggttt ctgggcaacg gcatgattca cggcgtcgag
     3061 ctgaatgacg gtcgggcgaa ctggtaccgc aaccgctatg tccgcactcc cctgctcagc
     3121 gacggggacc acagcgatgg ggtggacgag cgcgccaagt cccttgccaa cacccacatc
     3181 attggccacg cgggccggat tctagcgctg gaagaaggac actggccctt cgagctgacc
     3241 ccggagctcg acaccgttgg tccttatact ttcggcggcc ggctggagac cggcatgacg
     3301 gcccatccga agatctgcgc ggagaccggc gagctgctct tctttgctta cgcggcgacg
     3361 ccccccttca tgacctatca ccgcgcgacc gccgatggcg agctcgttca gagcgaggtc
     3421 atcgaggtca agggcgcgac catggtccac gacttcaaca tcacgcggaa ctacgtgatc
     3481 ttcatggacc tgcccgtggt gtggaatttc gagaacgccg cctctgtcgg tcttccgctg
     3541 gcctggagcg acgagtacgg cgcgcgcctc ggcgtgatgc cccgcaacgg ctccaacgcc
     3601 gacgtggtgt ggtacgacat cgatccctgc tacgtctacc acccgctgaa tgcctatgag
     3661 gacggcgacg acatcgtcat tgacgtctgt cgcatggccc accacatgaa gatcggggcg
     3721 ccggaagcgc cgccgatgct gcatcgctgg accattcatc agtcggcggg caccgtcagc
     3781 gaagcgcagc tggacgagcg gtcggtagat ttcccgcgcg tgcccgatgc gctggtcggt
     3841 cagaagcatc gctacggcta cacggcggag ttcggtggtg ggcttcccgc cgcggtggcc
     3901 ttccgtaagt acgacctggc cacgggaaca tccgaggcgc acgtgctggc cgacggccgt
     3961 gcgggcggcg agccggtctt cgttccggca cgagggggta cgtccgaaga cgatggatat
     4021 ctcatgagct tcgtctacga cccggcggac aaccgcagcg agctggtgat catcgatgcc
     4081 agcaacgtca ccgccgaccc cgtcgcgagg gtgcacctgc ccctgcgggt gccggccgga
     4141 ttccacggca gctgggtcgc ggacgcccag tagccgcctt aatggcgaac aacaacgtga
     4201 cgaggagacg acgttgatca ataagaaggt agtgctggca tcacgcccgg aaggccagcc
     4261 gaaggagtcg gatttcaagg tcatcgagga ggagctggcg gagctgaccg aagggcaggt
     4321 gctgctggcg acggagcatc tctcaatcga cgccttcatc agaacgatgc tggactccca
     4381 ggaagggctc catggcacgg tggccatcga tacgcccgtg atggccctgg gcgtggcgcg
     4441 cgtcgtggac agccgctcgc ccgacctgaa ccagggtgat gccgtgttcg gccctatggg
     4501 ggcacagact cacctcgtcg cgccgggcgg catgtaccgg aagctcgagg agggcgacgt
     4561 gccgccgcgc agctatctcg gcgcgctggg gatgaccacg ggccttacgg cctatgccgg
     4621 catgctcaag gtgggacagg tggccgaagg cgacaccgtg gtggtttccg ccgccgcggg
     4681 tgcggtggga accatcgcgt gccagctggc caagatagcc ggcgccaaag tcatcggcat
     4741 cgccggcggg ccggccaagg ttgcctacct gatcgacgag atcggttgcg atggtgccat
     4801 cgactacaag aacgacgacg tggcagagaa gctaagagcc cttgcgcctg ccggcgtgaa
     4861 cgtgttcttc gacaacgtgg gcggcgagct gctcgacatc gtgctcgatc agattgccca
     4921 gggagccagg gtggtgatct gcggcgccat ctctcagtac aaccacatgg aagaggtcag
     4981 ggggccgcgc ctttatctca ggatacccga gcgcaatgcc agcatgctgg gcttcaccgt
     5041 ggaccgcttt gcggcggact ttccggagat ggaggccgac atctcgcgct ggatggccga
     5101 aggaaagctc aagatgcccg agcacgtcgt cgagggcatc gagcagttcc ccaaagcgct
     5161 gaccatgctg ttcacgggcg gccacatggg taagctcctg gtcgctccgt gatccggcac
     5221 gacgccgcac gcgcgcggcg gcttttgccg gaccatcgct ccggggcaaa gccgcggcgc
     5281 gtgcgcggga tattcctcgc gctggttgcc gtttgcctgc aggtgcccgt cttggcggtc
     5341 gccgctgaga tcgacttcac ccgccatctg gtacgggtcg aaagcgcggc cgggcagcac
     5401 cccgggcccg atgtcgggcc ggcgcggccg ttcttcgggg ttgccggccc tgcccgggtt
     5461 atcgtaaccg cacgatccgg tgagccggtg gtgaccgtcc ggctgaacgg cgtcgacgtg
     5521 ctcgacgggc aggcgttacg gcaaacgacc tccctggaga tcccggtaga gcttgccgag
     5581 gcgaacacca ttgccgttgg cctcgacggc cgggacggcg ccgcggcgtc ggtgcgcgtc
     5641 aagcagcgcg cggacgtcgc gctgaacgtc atctcctggg tgcacttcaa caccaacgtg
     5701 agcgatttca acgaagcccg cgcgttttac ggcgtgctcg ggttcgaaac catgagcggc
     5761 tttccggaca ccaacaccct ggcgatggcc cacgccctgg gcatccagac gccgacggcc
     5821 tacgacggct cccagggaga agaggccggc ggctacctgc tccacggcga gctggtgggc
     5881 cccgatggtt tctggggcgg cgtcatcgat ctcatcgagt tcaccattcc gagaaacgaa
     5941 gagccgccgt acccgggcct taaccacctg ggcatggcgc gggctctcat gcacacgggc
     6001 gacattgcgg ccgcctacga ccagctcgtg ggagccggcg taaactttct ctctccgcct
     6061 gccacccgct cggacggtac ccgttttgcc gtcttcaccg atccggacgg caccttctac
     6121 gagctggtag agctggaagg cccaggcggc gaggcatccg agaaacccgt catcggtctc
     6181 ggtcgggtga acgtcaacgt gagcgacttc gaacgctccg aagcgtggta ccggatgttc
     6241 ggatacgtag ccacccgcaa gctgcctccg acggactccg ccgaggtggc agcggccatg
     6301 gggttcgatg cgccctacag gatcgacggg gccgtgctca gccacgaggt ggacggctcg
     6361 cagatcgagc tggtccagtg gctttcgccc ttcgaccccg agtccgcgta cccgccgccg
     6421 gtcagccacc tcggcattca tcgcatggcg ctgctcaccg gcgacatcga ggccgacgtg
     6481 gctgcgctca aggcccaggg tgttaagttc atctccggga tcaccccgtg ctgct
//
LOCUS       DBNHKV010000486         2894 bp    DNA     linear   ENV 01-MAY-2026
DEFINITION  MAG TPA_asm: Candidatus Azotimanducaceae bacterium isolate MAG04830
            SRR25158313_k127_212433, whole genome shotgun sequence.
ACCESSION   DBNHKV010000486 DBNHKV010000000
VERSION     DBNHKV010000486.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414238
            Sequence Read Archive: SRR25158313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Azotimanducaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Azotimanducaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Candidatus Azotimanducaceae.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 03:34:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,180
            CDSs (total)                      :: 3,154
            Genes (coding)                    :: 3,136
            CDSs (with protein)               :: 3,136
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 2 of 18
            Pseudo Genes (incomplete)         :: 17 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Candidatus Azotimanducaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158313_k127_212433"
                     /isolate="MAG04830"
                     /isolation_source="soil"
                     /db_xref="taxon:3107047"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.39 N 121.27 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="AC16PP_15885"
     CDS             <1..602
                     /locus_tag="AC16PP_15885"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR6483163.1"
                     /translation="SKPDPLTEAEEAAFRGHVEAGVEMIRQSTLNDRKVVMMVATHHE
                     RIDGSGYPRGLAGDDIPTFGQIAGIIDTYDAMITERRYAPAMSSYEAVYQLQTYAGIK
                     FQEELVEQLTQAIGVFPTGTLVELNTGEVGVVIAQNPERRLRPKINLILNEEKNLRRL
                     PIITDLNAMDTDPATPPRVWIKRELPHGAYGIEATDYFI"
     gene            complement(659..1522)
                     /locus_tag="AC16PP_15890"
     CDS             complement(659..1522)
                     /locus_tag="AC16PP_15890"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR6483164.1"
                     /translation="MSRSTFDVRPLDASFGAVVTGLRLADLGDDAFFDLYDAWLQHAL
                     LIFPDQHLSNAEQVRFAQRFGALEFDLTPLSNVRGDGSLRPDDGSDDVVKVLRGNMGW
                     HCDSTYMPVQAKGAVFTAHVVPDEGGETGWADMRAAYDALDEEMRERIADLCAYHSLY
                     HSQAKVGHVPSSESAYSNYGFHEGEPPLRPLVKIHPETGRPALMVGRHAYGIPGMDAD
                     ASERLLDELVEFACRPPRIYHHTWSPGDAVVWDNRCLMHRACPWDMSKPRVMYHARIA
                     GDPASESGLAA"
     gene            complement(1568..2197)
                     /locus_tag="AC16PP_15895"
     CDS             complement(1568..2197)
                     /locus_tag="AC16PP_15895"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR6483165.1"
                     /translation="MIRIYHVAGTRSVRVIWLCEELGLPYEKVAVDFSPAYRATPEWR
                     RLNPVGKVPVLVDDDVTMFESGAMVEHLLDRHGAGRLRPPPGTAERAAYLQWSWFAEA
                     TFARPLGEIVNHRREFPGESEIPAVLEEMSARAALCVQALDEALAGRQYLLGDEFTAA
                     DVMMGYSVLLARALGIEGARAGRVGAYFDVLARRSGYPAATADLPDAWR"
     gene            2305..>2894
                     /locus_tag="AC16PP_15900"
     CDS             2305..>2894
                     /locus_tag="AC16PP_15900"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6483166.1"
                     /translation="MGRPTDLGVIDLMLEMPTGTAGMGMKQARALTRDKGTGEFSHHP
                     AQYLFKDAPERMSLATDPDAVVGMMDDFGVAMAQIAVNPHKPEAALDLFEKHPGRFFG
                     EVGVDPNSGMEGVRALETCVGLHPNIKAASAAPCLLNPQVPIDDKKFYPIYAKCCELD
                     IPINMLVGVPGPRVPYRCQHPGLLDEVAWFFPELKVV"
BASE COUNT          555 a          966 c          894 g          479 t
ORIGIN      
        1 tttccaaacc ggacccgctg accgaggcgg aagaggcggc cttccgcggg cacgtggagg
       61 ccggcgtcga gatgatccgg caatccacct tgaacgaccg aaaggtcgtc atgatggtgg
      121 ccactcatca cgagaggatc gacgggtcag gctatccccg ggggctcgcc ggtgacgaca
      181 tcccgacctt cggccagatc gccgggatca tcgacaccta cgacgcgatg atcaccgaac
      241 gccgttacgc tccggccatg tcctcttacg aggccgtcta ccagctgcag acgtatgccg
      301 gcatcaaatt ccaggaggag ctcgtcgagc agctgaccca ggccatcggc gtgtttccca
      361 cgggcacgct cgtggagctc aacacgggcg aagtgggcgt ggtcatcgcg cagaaccccg
      421 agcggcgtct gcgacccaag atcaacctca ttctgaacga ggagaagaac ctgcgccgcc
      481 tgcccatcat caccgacctc aacgccatgg acaccgatcc cgccacgccc cccagggtct
      541 ggatcaagcg ggagttgcct cacggcgcgt acggaatcga agccaccgac tacttcatct
      601 gagggcaggt ctcgatgcgc ttgacgccga tcttgcgagc ggggggcaga gcgaccgctc
      661 acgcggccag tccggactcc gaggcagggt cgccggcaat acgggcgtga tacatcaccc
      721 gcggcttgct catgtcccac ggacacgccc gatgcatcag acagcggttg tcccacacca
      781 ccgcatcacc cggcgaccac gtgtggtgat agatgcgcgg cgggcggcag gcaaactcga
      841 cgagctcgtc aagcagacgc tctgaagcat cggcatccat gcccggaatg ccgtaggcat
      901 ggcggcccac catgagagcc ggccggcctg tctccgggtg aatcttcacg agagggcgca
      961 gaggcggctc gccctcgtga aacccgtagt tgctgtaggc gctttcgctg cttggcacat
     1021 gacccacctt cgcctggcta tggtagagcg agtggtaggc acacaggtcg gcaatgcgct
     1081 ccctcatctc ctcgtccagc gcatcgtaag cggcacgcat gtccgcccag cccgtctcgc
     1141 cgccttcgtc cgggacgacg tgggcggtga acaccgcgcc ttttgcctgg acgggcatgt
     1201 aggtgctgtc gcaatgccag cccatgttgc cgcgaagtac cttgaccacg tcatcgctgc
     1261 cgtcatcggg gcgcaggctg ccgtccccgc gcacgttgct cagcggcgtg agatcgaact
     1321 ccagcgctcc gaaccgctgc gcaaatcgga cctgctcggc gttgctcagg tgctgatcgg
     1381 ggaagatgag cagcgcgtgc tgcagccatg cgtcataaag gtcgaaaaaa gcatcatccc
     1441 caaggtctgc aaggcgcaag ccggtcacca cggcgccgaa cgaggcgtcc agcggccgaa
     1501 catcaaatgt cgatctgctc atgcttttcc tccggttctt catcgctcat tccggggcgc
     1561 ggcagcgtca gcgccaggcg tcgggaaggt ccgccgttgc cgcaggatat ccggaccttc
     1621 gggccagcac gtcgaagtat gcgcccacac gaccggcccg ggccccctca attcccagag
     1681 cacgcgccag cagaacggaa tagcccatca tcacgtccgc cgccgtgaac tcgtcgccaa
     1741 gcagatactg gcggccggcg agggcttcgt ccagcgcctg aacacagagg gcagcacggg
     1801 cgctcatctc ctccaacaca gccggaattt ctgattcgcc gggaaactcc cggcgatggt
     1861 tgacgatctc gccgagcggc cgggcaaagg tggcttccgc aaaccaggac cattgcagat
     1921 aggcggcacg ctcggcagta cccggcgggg gacgcagccg acccgctccg tgacggtcga
     1981 gcagatgctc gaccatggca ccggattcga acatggtgac gtcgtcgtcg acgagcacgg
     2041 gcactttgcc taccggattg agccttcgcc attcgggggt tgcccggtag gccggagaga
     2101 agtcgacggc caccttctcg taaggcagcc cgagctcctc gcacaaccag atgacccgga
     2161 ccgatctggt gccggcaacg tgataaatcc tgatcatgcg ggcttcccag ccatggcttg
     2221 cggccttcaa tcatccgcaa cagcgcgccg cgcggcaacc ggttaaactg agcgcgcttt
     2281 ggagacagcc gacggggagc acggatgggg cgaccgacag atcttggggt gatcgacctg
     2341 atgctcgaga tgcccaccgg cactgccggc atgggcatga agcaggcccg tgcattgaca
     2401 agggacaagg gaacgggcga gttctcccac catccggccc agtacctctt caaggacgcc
     2461 ccggaacgca tgtctttggc caccgatccc gacgcggtgg tgggcatgat ggacgatttc
     2521 ggcgtggcca tggcgcagat cgccgtcaac cctcataaac ccgaggccgc cctggatctt
     2581 ttcgagaagc atcccggccg attcttcggc gaggtaggcg tcgatcccaa ctcgggtatg
     2641 gagggtgttc gcgcgctcga gacctgcgtc gggctgcacc ccaacatcaa ggccgcctcc
     2701 gccgcgccct gcctgctgaa tcctcaggtg cccatcgacg acaagaagtt ctatcccatc
     2761 tacgcgaagt gctgcgagct cgacattccc atcaacatgc tggtcggcgt gccgggacca
     2821 cgcgttccct acaggtgcca gcatccgggc ctgctcgacg aggtggcctg gtttttcccg
     2881 gagctgaagg tggt
//
